LOCUS       DAJTSI010000001        71259 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000001 DAJTSI010000000
VERSION     DAJTSI010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 71259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71259
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.001"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..529)
                     /locus_tag="OO327_000001"
     CDS             complement(<1..529)
                     /locus_tag="OO327_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6016693.1"
                     /translation="MKIEYTPERGRGFVRPGETGKPQNWGFSGIKKAAPKWSRLSEQI
                     TRCAVCVCDPKHKHGDDSRYQAGGQCNQSGSVRCHNCEVLSFDEYSLRYFSRAKEHGA
                     NRSDSCSFFLRRLFRAGDVFSVDSSLVIVCACKAMRRSTSHHGADSFYPLGSMPRCCN
                     SLRIIRFIQAARESSP"
     gene            complement(587..775)
                     /locus_tag="OO327_000002"
     CDS             complement(587..775)
                     /locus_tag="OO327_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR6016694.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(1150..2379)
                     /locus_tag="OO327_000003"
     CDS             complement(1150..2379)
                     /locus_tag="OO327_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6016695.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            2628..3749
                     /gene="roxA"
                     /locus_tag="OO327_000004"
                     /gene_synonym="ycfD"
     CDS             2628..3749
                     /gene="roxA"
                     /locus_tag="OO327_000004"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR6016696.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(3798..5024)
                     /gene="pepT"
                     /locus_tag="OO327_000005"
     CDS             complement(3798..5024)
                     /gene="pepT"
                     /locus_tag="OO327_000005"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR6016697.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            5274..6410
                     /gene="potA"
                     /locus_tag="OO327_000006"
     CDS             5274..6410
                     /gene="potA"
                     /locus_tag="OO327_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR6016698.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            6394..7251
                     /gene="potB"
                     /locus_tag="OO327_000007"
     CDS             6394..7251
                     /gene="potB"
                     /locus_tag="OO327_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR6016699.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            7248..8042
                     /gene="potC"
                     /locus_tag="OO327_000008"
     CDS             7248..8042
                     /gene="potC"
                     /locus_tag="OO327_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR6016700.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            8039..9085
                     /gene="potD"
                     /locus_tag="OO327_000009"
     CDS             8039..9085
                     /gene="potD"
                     /locus_tag="OO327_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR6016701.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(9241..10062)
                     /gene="cobB"
                     /locus_tag="OO327_000010"
     CDS             complement(9241..10062)
                     /gene="cobB"
                     /locus_tag="OO327_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR6016702.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(10078..10989)
                     /gene="nagK"
                     /locus_tag="OO327_000011"
     CDS             complement(10078..10989)
                     /gene="nagK"
                     /locus_tag="OO327_000011"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR6016703.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(11018..12262)
                     /gene="lolE"
                     /locus_tag="OO327_000012"
     CDS             complement(11018..12262)
                     /gene="lolE"
                     /locus_tag="OO327_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR6016704.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(12262..12963)
                     /gene="lolD"
                     /locus_tag="OO327_000013"
     CDS             complement(12262..12963)
                     /gene="lolD"
                     /locus_tag="OO327_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR6016705.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(12956..14155)
                     /gene="lolC"
                     /locus_tag="OO327_000014"
     CDS             complement(12956..14155)
                     /gene="lolC"
                     /locus_tag="OO327_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR6016706.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            14417..15490
                     /locus_tag="OO327_000015"
     CDS             14417..15490
                     /locus_tag="OO327_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR6016707.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            15618..19064
                     /gene="mfd"
                     /locus_tag="OO327_000016"
     CDS             15618..19064
                     /gene="mfd"
                     /locus_tag="OO327_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR6016708.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            19207..20169
                     /gene="ldtC"
                     /locus_tag="OO327_000017"
     CDS             19207..20169
                     /gene="ldtC"
                     /locus_tag="OO327_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCR6016709.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(20252..20464)
                     /locus_tag="OO327_000018"
     CDS             complement(20252..20464)
                     /locus_tag="OO327_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6016710.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            20749..21381
                     /gene="comR"
                     /locus_tag="OO327_000019"
     CDS             20749..21381
                     /gene="comR"
                     /locus_tag="OO327_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR6016711.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(21443..21982)
                     /locus_tag="OO327_000020"
     CDS             complement(21443..21982)
                     /locus_tag="OO327_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6016712.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(22209..23513)
                     /gene="ndh"
                     /locus_tag="OO327_000021"
     CDS             complement(22209..23513)
                     /gene="ndh"
                     /locus_tag="OO327_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR6016713.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(23921..24463)
                     /locus_tag="OO327_000022"
     CDS             complement(23921..24463)
                     /locus_tag="OO327_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6016714.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(24486..25511)
                     /gene="nagZ"
                     /locus_tag="OO327_000023"
     CDS             complement(24486..25511)
                     /gene="nagZ"
                     /locus_tag="OO327_000023"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR6016715.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKVGH"
     gene            complement(25522..26346)
                     /gene="thiK"
                     /locus_tag="OO327_000024"
     CDS             complement(25522..26346)
                     /gene="thiK"
                     /locus_tag="OO327_000024"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR6016716.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(26327..26968)
                     /gene="lpoB"
                     /locus_tag="OO327_000025"
     CDS             complement(26327..26968)
                     /gene="lpoB"
                     /locus_tag="OO327_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR6016717.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(26983..27360)
                     /locus_tag="OO327_000026"
     CDS             complement(26983..27360)
                     /locus_tag="OO327_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR6016718.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTIYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(27363..27722)
                     /gene="hinT"
                     /locus_tag="OO327_000027"
     CDS             complement(27363..27722)
                     /gene="hinT"
                     /locus_tag="OO327_000027"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR6016719.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            28055..30240
                     /gene="fhuE"
                     /locus_tag="OO327_000028"
                     /pseudo
     CDS             28055..30240
                     /gene="fhuE"
                     /locus_tag="OO327_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(30300..31733)
                     /gene="ptsG"
                     /locus_tag="OO327_000029"
     CDS             complement(30300..31733)
                     /gene="ptsG"
                     /locus_tag="OO327_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR6016720.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(32028..32825)
                     /locus_tag="OO327_000030"
     CDS             complement(32028..32825)
                     /locus_tag="OO327_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6016721.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(32836..33840)
                     /gene="holB"
                     /locus_tag="OO327_000031"
     CDS             complement(32836..33840)
                     /gene="holB"
                     /locus_tag="OO327_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR6016722.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSCLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(33837..34478)
                     /locus_tag="OO327_000032"
     CDS             complement(33837..34478)
                     /locus_tag="OO327_000032"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR6016723.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(34468..35490)
                     /gene="yceG"
                     /locus_tag="OO327_000033"
     CDS             complement(34468..35490)
                     /gene="yceG"
                     /locus_tag="OO327_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR6016724.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(35493..36302)
                     /gene="pabC"
                     /locus_tag="OO327_000034"
     CDS             complement(35493..36302)
                     /gene="pabC"
                     /locus_tag="OO327_000034"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR6016725.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(36422..37663)
                     /gene="fabF"
                     /locus_tag="OO327_000035"
     CDS             complement(36422..37663)
                     /gene="fabF"
                     /locus_tag="OO327_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR6016726.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(37750..37986)
                     /gene="acpP"
                     /locus_tag="OO327_000036"
     CDS             complement(37750..37986)
                     /gene="acpP"
                     /locus_tag="OO327_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6016727.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(38197..38931)
                     /gene="fabG"
                     /locus_tag="OO327_000037"
     CDS             complement(38197..38931)
                     /gene="fabG"
                     /locus_tag="OO327_000037"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR6016728.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(38944..39873)
                     /gene="fabD"
                     /locus_tag="OO327_000038"
     CDS             complement(38944..39873)
                     /gene="fabD"
                     /locus_tag="OO327_000038"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR6016729.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(39889..40842)
                     /gene="fabH"
                     /locus_tag="OO327_000039"
     CDS             complement(39889..40842)
                     /gene="fabH"
                     /locus_tag="OO327_000039"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR6016730.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(40910..41980)
                     /gene="plsX"
                     /locus_tag="OO327_000040"
     CDS             complement(40910..41980)
                     /gene="plsX"
                     /locus_tag="OO327_000040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR6016731.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(42061..42234)
                     /gene="rpmF"
                     /locus_tag="OO327_000041"
     CDS             complement(42061..42234)
                     /gene="rpmF"
                     /locus_tag="OO327_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR6016732.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(42286..42807)
                     /gene="yceD"
                     /locus_tag="OO327_000042"
     CDS             complement(42286..42807)
                     /gene="yceD"
                     /locus_tag="OO327_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR6016733.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            43006..43590
                     /gene="yceF"
                     /locus_tag="OO327_000043"
     CDS             43006..43590
                     /gene="yceF"
                     /locus_tag="OO327_000043"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR6016734.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKSQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(43702..44661)
                     /gene="rluC"
                     /locus_tag="OO327_000044"
     CDS             complement(43702..44661)
                     /gene="rluC"
                     /locus_tag="OO327_000044"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR6016735.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            45234..48425
                     /gene="rne"
                     /locus_tag="OO327_000045"
     CDS             45234..48425
                     /gene="rne"
                     /locus_tag="OO327_000045"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR6016736.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQVPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPMVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(48622..49564)
                     /gene="flgL"
                     /locus_tag="OO327_000046"
                     /pseudo
     CDS             complement(48622..49564)
                     /gene="flgL"
                     /locus_tag="OO327_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
     gene            complement(49576..51218)
                     /gene="flgK"
                     /locus_tag="OO327_000047"
                     /pseudo
     CDS             complement(49576..51218)
                     /gene="flgK"
                     /locus_tag="OO327_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(51284..52225)
                     /gene="flgJ"
                     /locus_tag="OO327_000048"
     CDS             complement(51284..52225)
                     /gene="flgJ"
                     /locus_tag="OO327_000048"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCR6016737.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(52225..53322)
                     /gene="flgI"
                     /locus_tag="OO327_000049"
     CDS             complement(52225..53322)
                     /gene="flgI"
                     /locus_tag="OO327_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR6016738.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(53334..54032)
                     /gene="flgH"
                     /locus_tag="OO327_000050"
     CDS             complement(53334..54032)
                     /gene="flgH"
                     /locus_tag="OO327_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR6016739.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(54085..54867)
                     /gene="flgG"
                     /locus_tag="OO327_000051"
     CDS             complement(54085..54867)
                     /gene="flgG"
                     /locus_tag="OO327_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR6016740.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(55005..55756)
                     /gene="flgF"
                     /locus_tag="OO327_000052"
                     /pseudo
     CDS             complement(55005..55756)
                     /gene="flgF"
                     /locus_tag="OO327_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(55776..56915)
                     /locus_tag="OO327_000053"
     CDS             complement(55776..56915)
                     /locus_tag="OO327_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR6016741.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGANNIVATTQNGYKPGDLVSYQINDDGTV
                     VGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWSATQSSGVALLGTAGTGNFGTLTN
                     GALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQDQILNTRVNLR"
     gene            complement(56940..57635)
                     /gene="flgD"
                     /locus_tag="OO327_000054"
     CDS             complement(56940..57635)
                     /gene="flgD"
                     /locus_tag="OO327_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCR6016742.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(57647..58050)
                     /gene="flgC"
                     /locus_tag="OO327_000055"
                     /pseudo
     CDS             complement(57647..58050)
                     /gene="flgC"
                     /locus_tag="OO327_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(58054..58470)
                     /gene="flgB"
                     /locus_tag="OO327_000056"
     CDS             complement(58054..58470)
                     /gene="flgB"
                     /locus_tag="OO327_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR6016743.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            58625..59284
                     /gene="flgA"
                     /locus_tag="OO327_000057"
     CDS             58625..59284
                     /gene="flgA"
                     /locus_tag="OO327_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR6016744.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            59360..59653
                     /gene="flgM"
                     /locus_tag="OO327_000058"
     CDS             59360..59653
                     /gene="flgM"
                     /locus_tag="OO327_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR6016745.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            59658..60074
                     /gene="flgN"
                     /locus_tag="OO327_000059"
     CDS             59658..60074
                     /gene="flgN"
                     /locus_tag="OO327_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR6016746.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(60114..61649)
                     /gene="murJ"
                     /locus_tag="OO327_000060"
     CDS             complement(60114..61649)
                     /gene="murJ"
                     /locus_tag="OO327_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR6016747.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(61849..62682)
                     /locus_tag="OO327_000061"
     CDS             complement(61849..62682)
                     /locus_tag="OO327_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6016748.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     V"
     gene            complement(62684..63331)
                     /locus_tag="OO327_000062"
     CDS             complement(62684..63331)
                     /locus_tag="OO327_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR6016749.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(63342..63926)
                     /gene="rimJ"
                     /locus_tag="OO327_000063"
     CDS             complement(63342..63926)
                     /gene="rimJ"
                     /locus_tag="OO327_000063"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6016750.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            64162..65370
                     /gene="mdtH"
                     /locus_tag="OO327_000064"
     CDS             64162..65370
                     /gene="mdtH"
                     /locus_tag="OO327_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR6016751.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            65434..66081
                     /gene="grxB"
                     /locus_tag="OO327_000065"
     CDS             65434..66081
                     /gene="grxB"
                     /locus_tag="OO327_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR6016752.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            66215..66775
                     /locus_tag="OO327_000066"
     CDS             66215..66775
                     /locus_tag="OO327_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6016753.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            66881..67927
                     /gene="pyrC"
                     /locus_tag="OO327_000067"
     CDS             66881..67927
                     /gene="pyrC"
                     /locus_tag="OO327_000067"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR6016754.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            68001..68246
                     /gene="dinI"
                     /locus_tag="OO327_000068"
     CDS             68001..68246
                     /gene="dinI"
                     /locus_tag="OO327_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR6016755.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            68536..68790
                     /gene="bssS"
                     /locus_tag="OO327_000069"
     CDS             68536..68790
                     /gene="bssS"
                     /locus_tag="OO327_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR6016756.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            68905..70023
                     /gene="solA"
                     /locus_tag="OO327_000070"
     CDS             68905..70023
                     /gene="solA"
                     /locus_tag="OO327_000070"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR6016757.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            70071..70184
                     /locus_tag="OO327_000071"
     CDS             70071..70184
                     /locus_tag="OO327_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCR6016758.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            70445..71011
                     /locus_tag="OO327_000072"
     CDS             70445..71011
                     /locus_tag="OO327_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR6016759.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            71015..>71259
                     /locus_tag="OO327_000073"
     CDS             71015..>71259
                     /locus_tag="OO327_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR6016760.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDT"
BASE COUNT        17325 a        18964 c        17635 g        17335 t
ORIGIN      
        1 acggcgatga ttctcttgct gcctggataa agcgaataat ccgcaaagaa ttacagcaac
       61 gcggcataga acccaaagga taaaaactat cagcgccgtg gtgtgaggta ctacggcgca
      121 ttgctttaca ggcgcacaca atgaccaacg aagaatcaac cgaaaaaaca tcacctgcac
      181 gaaaaagacg acgcagaaag aaagagcatg aatcagaacg attcgcgccg tgttcttttg
      241 cccttgagaa gtaccttaaa gaatactcat caaaggagag aacttcgcag ttatggcaac
      301 gcactgaacc agactgattg cattgcccac cagcctgata gcggctatca tccccgtgct
      361 tatgtttggg atcacacaca caaacggcgc agcgggttat ctgttcagaa aggcggctcc
      421 atttcggggc cgcttttttt atgcctgaaa aaccccaatt ttgtggtttt ccagtttcac
      481 cagggcgaac gaatccccgc ccacgttctg gcgtatattc aatcttcatg gttatagctc
      541 tgtgttcaga tgattgatgt gtggcggctg cgtgccgcca gcgtgattaa tgaactgcct
      601 tgcagctatc cttccaggcc agaacctcgg ataaagacca gccaacggaa cgaccgccaa
      661 gtttacgacg tgatgggaat tgtccggcct tttccaggcg gtagcggcat gagcggctaa
      721 ggcctgttag cttttcgcat tctttttcac gtataaaccg atcagtgctt aacactattg
      781 ccccctttcg tttcttaaag agttatttcg tgttctattg cgttgggatg tgtctgattg
      841 tgtcaggatg attcagagtt ggcaaatgtt gaggccgtat ggtttacaga aagaggaata
      901 atcaggataa aatcctttaa attcatgtta atacaaaggc ataaaatatt gtttcatgcc
      961 ttttttctcg ctatttaaag agtgattcgc tagtgtataa aaaatcagta acacattaaa
     1021 aatcagctac ttataaatct gtacactttt tcacctcttg ttcgtagttg ttccgcattg
     1081 ttgctcattg ttgcacgttg tatctgttcg catatccagt atgcgcatac tgaaaaaaca
     1141 cgaaaaaaat tattttcttc tggctactgg tagcgtggtt acgttttcat gtgttcccgc
     1201 cagtatccct aaccgctccg tccacatatc cagcgcattg cgtttagcat ccagataacg
     1261 ggaatgatta taaactcgct gcattcctgg catctggtgg cctgtaagct gctccacgac
     1321 gtgaggatcc acgcctaaat cgttcagcat cgttgtaaag gtgcgccgga tgtcatgtag
     1381 tgaccagtga gggtgattta gcctcctgtg cgctaatctg ccgtactgcg acacgcttgt
     1441 ttcctgtttc acttccccca gcaataagcc cgtgtgcctg ttctgctcca ccagctgcgt
     1501 gacgaacggc agtattgctt ccggtatggg ccggaatatt gccaccttcg ttttgctgtg
     1561 ttctttcggc acggtccaga gcatttcggt aaaatcccac tcgctgatct ccgataacct
     1621 cagttctacc gtccggcatc cgaacacaat caggaggcgg attaacgcga tgtagtaggg
     1681 ggagaatatt tttttgtcca gtgcctgcaa taattcgccc agttctttgg tgcttaagac
     1741 acgctcgctt atatccggtt ttttcccaac gtccgccaca ctcatatcat caagaacgtt
     1801 gctgattgca tagcgccgcc tccggcagaa cttaagcgcc tgtttgcacg tctgtagcaa
     1861 gaatccggca gtaacaggcg ttcgctttgc cacctggtca aaacaggcca gccagtgccg
     1921 tagctcgcat ttatccagcg gcatagcacc aatgtgctgt attacgtgat tattaaggcg
     1981 ctttttcagg gcggcataat ccacgcggtt ttcctttgcg tacgactcaa gccagtaggt
     2041 gagcgcatcg ccaaccgtta ccggctttaa cgcttcctgt acggtgtaat taagctcatg
     2101 acgtggattt ttcccctctg ccagccatgc gcgacactgg gcggcttttt ccctggctga
     2161 tttcaggctc agatcaggat aatttcccag cttaatgcgt tccggtggtg ccccccttcc
     2221 cgttccggcc ctgtaagtga aataccaggt taaaaggcca ctggttgaat gcctgacgct
     2281 caggtttcca ccgtcattaa gaaaggctgt tttttgggcg ggtgtgccgt tgattttcct
     2341 cagctgtgta tcgctcagtt tgttaagtgc tctgctcata attttgattc cggtcatcac
     2401 aattaactac accattaact acaccgatcg ttgcacaaag ggctacaacg tgaaacaagc
     2461 tggaacaagg aaaatcacaa atctgtttat aatcaaaaac ataatgaaca atctgaaaca
     2521 ttatgaaacg gcaaaaaaca ctaattgata aaatatgtcc atacttcacg cattacgtta
     2581 agcatccgtt ataatcggtt gcagatacca gcctgtggat gcttaacatg gaataccaac
     2641 tcactcttaa ctggcccgat tttcttgaac gtcactggca gaaacgcccg gtggtgttaa
     2701 aacgcggctt taataatttt attgacccga tctctccaga cgagttggcg ggtctggcga
     2761 tggaaagcga agttgacagt cgactggtca gtcaccagga tggcaaatgg caggtcagcc
     2821 acggcccgtt cgaaagctac gatcatctcg gtgaaaccaa ctggtcatta ctggtacagg
     2881 cagtaaacca ctggcatgag ccgaccgccg cgctgatgcg accgttccgt gaactaccgg
     2941 actggcgtat tgatgatctg atgatttctt tttctgtacc tggcggcggc gtcggcccgc
     3001 atctcgatca gtacgacgtg tttatcattc agggtaccgg acgtcgtcgc tggcgagtgg
     3061 gcgaaaagct gcaaatgaaa cagcactgcc cacatccgga tctgttacag gtcgatccgt
     3121 tcgaagccat catcgatgaa gagctggagc ctggtgatat tctttatatt ccgccaggat
     3181 tcccgcatga aggctacgcg ctggaaaatg cgatgaacta ttccgtgggt tttcgcgcgc
     3241 caaatacgcg ggaattaatt agcggatttg ccgattatgt gctgcaacgt gaactgggcg
     3301 gcaactacta cagcgatccg gatgttccac ctcgtgctca tcctgcggat gttctgccgc
     3361 aagagatgga taaactgcgt gagatgatgc tcgaattgat caaccagccg gaacacttta
     3421 agcaatggtt tggcgagttt atatcccagt cacgtcatga actggatatc gcgccgccgg
     3481 aaccgcctta tcagccagat gaaatctacg atgcgctgaa acaaggtgat gtgctggtgc
     3541 gcctgggtgg tctgcgcgta ttgcgcattg gcgacgacgt gtatgccaat ggtgagaaga
     3601 tcgattcccc gcaccgtccg gcactggatg cactcgccag caacattgcg ctgactgcgg
     3661 agaattttgg cgatgcgctg gaagatccgt cattcctcgc gatgctcgcg gcgctggtca
     3721 atagcgggta ttggttcttc gaagggtaag tttggattta agccggatgc agcatcacat
     3781 gccgcatccc ttttcgctta cttccgttgc gccgttaact cggcaatacg gacgatcacc
     3841 tgcaccgctt tttccatacc ttccagaatc acaaactcat gcttaccatg atagttgtaa
     3901 ccgccagtga acaggttcgg gcaaggtaat cccataaacg acaactgcgc gccgtcggta
     3961 ccgccgcgga tcggtttcag ttccggttca atatcgcagt cacgcatcgc ctgctgggcg
     4021 atatcgagaa tatgcggatg ctcaaccact ttctcgcgca tattgtagta actgtcttca
     4081 atcaccaatt caatgtagca atcaggatgt aaccctttgc ccactttttt ggcgatctcc
     4141 atcattttac gtttacgcgc ttcaaactgt ttacggtcga aatcacggat gatgtagtgc
     4201 atatcggccc gttcaacggt gcctttcatg ctcgccaggt gatagaaacc ttcatagcct
     4261 tctgtcattt ccgggctttc atccgccgga acttccgcat gaatacgtgc cgccagcgac
     4321 agcgcattta ccatcactcc tttcgccgtg cccggatgaa cattgttacc gacaattttt
     4381 atattgaccg atgcggcgtt gaagttttca aactccagtt cgcctacgcc accaccgtca
     4441 acagtgtaag cccagcgggc atcgaaggca tcaacatcaa aatgtttcgc ccctttgccc
     4501 acttcttcat ccggggtaaa ggcgacgcga atatcaccat gcggaatgtt tttctgttgc
     4561 aataccgcca gcgcggtcat gatttctgca atacctgctt tgtcatcagc acctaacaag
     4621 gttttaccat cggtggtaat caacgtctga cccagtagct gatgcagcac cgggaacata
     4681 accggtgata aaacttcatc gccgataccc agcgcaatat cgccaccgcg atagttttca
     4741 acaatttgcg gattaacatt tttgccgctg caatccggtg aggtatccac atgagaaata
     4801 aagccaatcg ccgggatatc gccagggacg ttagccggta acgtcgccat caaagtgccc
     4861 ttctcactta aggtcacatt gataagcccc atttcttcga gctgctcttt cagcagatgc
     4921 aataacttcc attggccttc cgtgctggga acctgtctca cccctgcttt tgattgggta
     4981 tccagagaca cgtagttcaa aaatcgctca agtagtttat ccatgtagtc accctcactt
     5041 tttgtgacaa cattattaag aagcaagaaa agacaaatat tgcgtcaggt cacttttacc
     5101 cctgcaagcg ggaatattta tcagcattac cttaatgaat ataaagctaa gccagtaatt
     5161 cacaacaaag attggcgatt caagcggttt gccgtagaat taccgccctc attaggagtc
     5221 accaaggtgg ttaaccacaa accccgcatc ggtaagccat ccgttgcgtt tacatgggac
     5281 agagtaaaaa attgaataaa caaccgagtt cgctttcacc gctggtgcaa ttggcgggaa
     5341 ttcgcaaatg ctttgatggt aaagaggtca ttccccagct ggatctgact atcaacaatg
     5401 gcgagttcct cacgctgctt ggcccttctg gctgcggtaa aacaaccgtt cttcgcctga
     5461 ttgcaggtct ggaaactgtt gattccggac gcatcatgct ggataacgag gacatcaccc
     5521 acgttccggc ggaaaaccgc tatgtgaaca ctgttttcca aagctatgca cttttccccc
     5581 acatgaccgt gttcgaaaat gtggcctttg ggttgcgcat gcaaaaaacc cccgctgctg
     5641 aaattacgcc ccgcgtgatg gaagccctgc ggatggtgca gctggaaacc ttcgctcaac
     5701 gtaaaccgca tcagctctct ggtggtcaac agcaacgcgt cgccattgct cgagcggtgg
     5761 ttaacaagcc tcgtctgttg ttgctggatg agtcgctctc agcgctggat tacaaactgc
     5821 gtaagcaaat gcagaacgag ctgaaagcgt tacagcgtaa gcttggcatt acattcgttt
     5881 tcgtgactca cgaccaggaa gaagcactca ccatgtcgga caggattgtg gtgatgcgcg
     5941 atggtcgcat tgagcaagac ggcacgccgc gtgaaatcta cgaagagccg aaaaacctgt
     6001 ttgttgccgg gttcattggc gaaattaata tgtttaacgc cacggtgatc gagcgactcg
     6061 acgagcagcg cgtacgcgcc aacgttgaag gccgcgaatg taatatctac gttaacttcg
     6121 ccgttgaacc ggggcaaaaa ctgcatgttc tgctgcgccc ggaagactta cgtgttgaag
     6181 agattaacga cgacaaccac gctgaagggc tgattggtta cgttcgtgag cgtaactaca
     6241 aaggcatgac gctggagtcg gttgttgaac tggaaaatgg caagatggtg atggtcagcg
     6301 aattcttcaa tgaagacgat cctgactttg accactctct cgaccaaaaa atggccatta
     6361 attgggtaga aagctgggag gtcgtactgg ctgatgaaga acacaagtaa gttccagaat
     6421 gtagtgattg tcactattgt cggttggctt gtgttgtttg tctttctgcc caacctgatg
     6481 atcattggca ccagcttttt gacccgcgac gacgccagtt tcgtcaaaat ggtctttaca
     6541 ctggataact acacgcgtct gctcgatccg ctctattttg aagtgctatt gcactcgctg
     6601 aatatggcgc tgatcgccac cctcgcctgc ctggtgctgg gctatccatt tgcctggttt
     6661 ctggcgaagc tgccacacaa ggtgcgtccg ctgctgctgt ttctgctgat tgttccgttc
     6721 tggaccaact cattgattcg tatctacggg ctgaaaattt tcctcagcac caaaggctat
     6781 ctcaacgagt ttttgctctg gctgggcgtt atcgacacac caatccgtat catgttcacg
     6841 cccagtgcgg tgattatcgg tctggtttac attctgctgc cgtttatggt gatgccgctg
     6901 tactccagta tcgaaaaact ggataagccg ttgcttgagg cggcgcgcga tctcggtgcc
     6961 agcaagttac agacttttat ccgcatcatt attccactga cgatgccggg gattattgcc
     7021 ggatgtctgc tggtgatgct gccagcgatg ggcctgttct atgtgtccga cctgatgggc
     7081 ggtgcgaaaa acctgctgat cggtaacgtc atcaaggtac agttcctcaa tattcgtgac
     7141 tggccgtttg gtgcagccac cagcattacg ctgactatcg taatgggcct gatgttgctg
     7201 gtttactggc gcgcttctcg tttgctgaat aagaaggtgg aactcgaatg atcggtcgac
     7261 tgcttcgcgg cggttttatg accgctatct acgcgtacct gtatatccca atcattattt
     7321 tgattgtgaa ctcctttaac agctcgcgct ttggcatcaa ctggcagggt tttaccacca
     7381 aatggtatag cctgctgatg aacaacgaca gcctgttaca ggcagcgcag cattcactaa
     7441 caatggcggt gttttcggcg acgtttgcta cgcttatcgg ttcactgacg gcagttgcgc
     7501 tgtaccgtta tcgctttcgc ggtaagccgt tcgttagcgg aatgctgttt gtggtaatga
     7561 tgtcaccaga tatcgtgatg gcaatttcgc tgctggtgct gtttatgctg ctgggtattc
     7621 agcttggctt ctggtcgctg ctgttctcgc atatcacctt ctgcctgcca tttgtggtgg
     7681 tgacggtgta ttcgcgcctg aaaggttttg acgtgcggat gctggaagcg gcgaaagatc
     7741 tcggtgccag cgaatttacc attctgcgaa aaatcattct gccactggca atgccagcgg
     7801 tggcggcggg ctgggtgtta agctttaccc tgtcgatgga cgatgtggta gtttcttcat
     7861 tcgtcaccgg accaagttat gaaattctgc cgttaaaaat ttattcgatg gtcaaagtcg
     7921 gcgtatcgcc ggaagttaac gcgctggcaa ccatattact ggtgctgtcg ctggtgatgg
     7981 ttattgccag ccagcttatt gctcgtgata aaacgaaagg taacacaggg gacgttaaat
     8041 gaaaaaatgg tcacgccacc tgctcgcggc gggtgctctg gcactgggca tgagcgccgc
     8101 tcacgccgat gacaacaaca cgctgtattt ctacaactgg accgagtacg tgccgccagg
     8161 actgcttgaa cagttcacca aagaaaccgg tattaaggtt atctattcga cttacgagtc
     8221 gaacgaaacc atgtacgcca agctgaaaac gtacaaagac ggtgcctatg acctggtggt
     8281 tccttcaacc tattacgtcg ataaaatgcg taaagagggg atgatccaga agatcgacaa
     8341 gtcgaagtta acgaacttca gcaatctcga tccagacatg ctcaacaagc cttttgaccc
     8401 gaataacgac tactccattc cgtatatctg gggtgcgacg gcgattggtg ttaacggtga
     8461 tgcggtggat ccgaaatctg tcaccagctg ggccgatctg tggaagccag agtacaaagg
     8521 cagcctgctg ttgaccgacg atgcccgtga agtgttccag atggcgctgc gtaagctggg
     8581 ctactccggt aacaccaccg atccgaaaga gattgaagct gcatataacg agctgaaaaa
     8641 actgatgcca aacgtcgcag cgtttaactc cgataacccg gctaacccgt acatggaagg
     8701 cgaagttaac ctcggcatga tctggaacgg ttctgctttc gttgcacgcc aggcgggtac
     8761 gcctattgac gtggtgtggc cgaaagaagg cggcatattc tggatggaca gcctggcgat
     8821 cccggcaaat gccaaaaaca aagaaggcgc gctgaaattg atcaacttcc tgctgcgccc
     8881 ggatgtggca aaacaggttg ctgaaactat cggttatcca acgccaaacc ttgcggcgcg
     8941 taagctgtta agtccagaag tggcaaacga taagacgctc tacccggatg ctgaaaccat
     9001 taaaaatggc gaatggcaga atgacgttgg cgcagccagc agcatttatg aagagtatta
     9061 tcagaagctg aaagcaggac gttaattcag caagcgggcg gtggtattac cgcccgtttt
     9121 ttagttagca aggatgtggc taatgagtca ggacagtaaa gttttatccc gctctttcgt
     9181 cggtattgtg gtccgcttgt aggcctgata agcgtagcgc atcaggcaat gcttcctgct
     9241 ttataatccc tccagcaact tttcaacaaa ttctggcacc acctggcttg ccgggccgta
     9301 atatttctcg gcaaattcat taccaacctg actcggttca agattcagtt caacggtgtg
     9361 agcaccatgt agtttggctt cgtgtacaaa cccagccgcc ggatagacat gcccggaagt
     9421 accaatggca ataaagacat ccgccatcga caacgccata taaatttcat ccatgccgag
     9481 tggcatttcg ccaaaccaaa cgacgtgcgg gcgcaagggt gccgggaact ggcaacaatg
     9541 gcatttatct tctggcgtaa cgtctccggt ccagtcgaga acctgaccac tttgcgaaca
     9601 acgaactttc agcaattccc catgcatatg aatgacattg gtattacctg cgcgttcatg
     9661 caggttgtct atattctgcg ttaccagcaa aaagcgatcg ccgagagcgt cttgcagttt
     9721 agccagcgca agatgcgcgg cgttaggctg aatttctggc tgctgcagct gtcgacgacg
     9781 ggcgttataa aacgcttgca ccagttcagg atcgcgatcg aaaccttccg gcgttgccac
     9841 atcttcaacc cgatgttctt cccacaggcc atctgcggcg cgaaaggtac gaatacctga
     9901 ttccgcagaa attcctgccc ctgtcagtac gagtactctt ggtttttcca ttgcttccgg
     9961 caccacttta tctctgaaaa aaatacgctg acgcaaacgc tcgcgcaggc gacgtttatt
    10021 tttacgaaaa cgacttaacc gatgaccccg acgcgacagc atagcaacct ctgttgttta
    10081 atcggttaga tgtaggaagg ccgcaccacg cattcctccc gcatcaccgt ggcgcgcgcg
    10141 ttcaatgcgt ggaacacgag ctacaggtaa gagatgacaa ggcaacctgt ccgccagttg
    10201 cgttgtgatt gccgggaaat tcgataagcc accaccaatg acgaccaggt cagggtcaac
    10261 aatggtcagg atatttccca gagaaaccgc taataaatcc agataacgct caacgtgcgc
    10321 ccttgcctgc tcatcgcctt gatcataaag cgcaataatt tcgggagcct gcaacggttg
    10381 atgataatag tgttgataca gccacgcaaa accgcgacca gacagataat tttcaatgca
    10441 gccatgctga ccacagccgc agcggcgtaa cgggaaatcc aaccccatca tggttaacgc
    10501 atcaaccggc agacgcatat ggccaaactc gccggtaatg tagcttttac cagtaatcgg
    10561 tttgccgttg aaaaccagcc cgccgccaac gccggtgccg agaattaacc ccatcaccag
    10621 tggatattgc gtaaattcat catcccaggc ttctgaaagg gcaaaacagt tggcatcgtt
    10681 atcaaggcgt acatcgcgat caagacgtgc gctcaggtcg gcacgcagcg gtttgccgct
    10741 ggcagcgggg acattagcgg catacagctt accatcttct gtttccggca ttcccggaat
    10801 accgatgccg acagaacctt tacagccaaa acgttggtca gcttcagcta ccagctcaca
    10861 cactgcatct aaaaatgcgt catagctgtc acgcggtgtc ggcacccgct tttcccactg
    10921 caactgccga ccgctatcaa acacgccaag cgcaattttt gttccaccaa tatcaaaccc
    10981 gtaatacatt gccgctcctt gttttaatgt actgccttta ctggccgcta aggactcgcg
    11041 cagggtcaat attactggcg cgccgcgccg gataccaact tgccaaaaga ctcagcaaca
    11101 atgctgtgac cagtacgtag aagacgtcca gccagtgcaa ttccgatggc aggaagtcaa
    11161 taaaataaat atcgctggag aggaactgat gaccgatcag cttttcaatc cactcaataa
    11221 tcggggtaag ttgcagtgaa acaactacgc cgataaccac cccacacagg ctgccgaaca
    11281 gccctgccag caatccatac cagacaaaga tggcgcgaat taaaccatct ttcgccccca
    11341 gcgttcttaa tactgcgata tcgccactct tgtctttcac cgccatcact aaggtggaga
    11401 cgatgttgaa acaggccacg ccaatcacca gtaccatcgc cagatacata atggcgcgga
    11461 tcatctggat atcgcgatac atatagccgt aagtaccaat ccaactttta atataaacat
    11521 agctgttggt cacttcaccc gcatcgcgta ccagcttatt ggcgttgaaa acatccgtca
    11581 ttttaagggc aatacctgac acgctggaac ccatatcaag atattgttgg gcatccgcca
    11641 gcgggatcat ggcaaaactg tgatcgagtt gaccactcaa ctgcaaaata ccggcaatgt
    11701 gcaaacgcac acgttttggc tgcatcagtt tatgctcggg attcgagttg gggatcatga
    11761 tcgacaccca atcgccctgc ttcactttca gcgcatccgc cacgcctttg ccgatgataa
    11821 tttgctgttc gcccgcttta aaattgcgcc acgcatcccc ctgaacaaac gagggtaatg
    11881 cgctcagacg ctgttcctgt tgtgggttaa cgcccttcac ctggattgcg cgcagattcg
    11941 ctccactttc caccagaccg gtgaaattga tatacggcgc agcggcggca atacccggca
    12001 ctttctgcac gttatccagt gcttcctgcc agttagtcca tggctgattt accgcttcga
    12061 tttcgccatg cggcaccacc gccagaatgc ggttattcag ttcgcgttca aagccgttca
    12121 tcgcgcttaa gccgacgatc aataccgcca cgccgagggc aatgccaatg gtagaaatga
    12181 cagagattag cgacaccatg ccgccgcgtc gccgtccgcg actaaaacgc aggccaatca
    12241 ataacgataa aggcatcgcc attactccgc ccccatcagg ctcagttccg ccgtcagacg
    12301 tccatcacgc atttccagtt ggcggctcat acgtttcgcc agttgcaggt cgtgagtgac
    12361 caccaggaaa gcggtgccct gcaagcgatt caactcccca agcaactgga agatgctgtc
    12421 ggcgttacgc gcatcgaggt taccggtagg ttcatccgcc agtaccagac gcggattatt
    12481 gaccagcgca cgggcaatcg ctacacgctg gcgttcgccg ccagaaagtt cagatgggcg
    12541 gtgattcgca cgatgctcca gccccaccgc ttttaacatc tccagtgcac agctgttgat
    12601 ttcagcgggc tttttcttgc caatcagcag tggcatagcc acgttttcca gggcagtaaa
    12661 atccggcagc aggtggtgaa actgataaat aaagcccagc ttctggttgc gcagttctgc
    12721 tttcgccgct gaagacagtt tgctcatcgg ctgaccgtta aagatcacat cgccggaggt
    12781 tggcgtatcc agcccgccta acaggtgcag caaggtactt ttaccggaac cagagctacc
    12841 gacaattgcc atcatttcac cttcgcccac gctgaaactg acattgagca gcacatcggt
    12901 ttgcacactg ccttcctgat agcgtttgca caggttgtcg cattgcaaca ggatcttatt
    12961 cataacgtaa agcctcagcg ggttgtgtgg cggcagcgcg ccatgaaggg taaagcgtag
    13021 acagcagcgc gatagccatc gccaccagcg caataacaat gacctgtaaa ggttcgatag
    13081 ccaccggcag cgccgcgcca tcaagcagga cgccgattat cggcatcaga ttatttaact
    13141 ggctggcaag cagtgcgcca agcgccgctc cgaggattgc accgataatc ccggcgctag
    13201 ccccttgcac cataaagacc atcatgattt gtcgcggagt taagccttgc gtttgcagga
    13261 tcgcgacttc gccctgcttc tccatcacca tcagccccag cgaggtaata atgttaaacg
    13321 ccgcaacagc gacaatcagg ctcagcagta agcccatcat atttttttcc atgcgtacgg
    13381 cctggaacag ctcgccttta cggtcacgcc agtcctgcca tttgctgcct tcaggcagtt
    13441 tttgctgact taaagagtca actctcagcg gctcatccag ccacaaacgc cagccggtaa
    13501 tattggctgc cggataacgc atcagacgcg aggcatcctc aatattcacc agcatttcat
    13561 agccatcgac ttcactgttg gcggcgaaag tgccaatcac attgaacagg cgctgacttg
    13621 gaatacgccc catcggcgtg aactggctgg cagatggcac catcacgcgg atttgatcac
    13681 cgcgattgac gcccaactgt gaggcaagtt gttcgccgag gatgacatta tatttccccg
    13741 gctcgaggtc agtttgtttc acattgacca gatacggcgt tagtggatct ttttgcgccg
    13801 ggtcgatacc gagcatcacc ccgaccgcca cgctgcgcgc gctttgcagt accacatcac
    13861 cggtagtaat aggtgcgacg cgattaacgc cgtccagttt gactgccgtt tccgggagtt
    13921 gctgcgggtt aagagagcca tgctcagaag agagaattgc ctgtggcatc aggccaagga
    13981 tgttgttttg cagctcgcgc tcaaagccgt tcatcactga caatactgtg accagcgcca
    14041 tcaccccgag ggtaatgccg atggtagaaa gccaggagac gaaacgaccg aagcgatcca
    14101 ctgcacgccc acgcatgtaa cgcaggccaa taaatagagc gacaggttgg tacatgaaat
    14161 ccgtctggtt gctgtagcaa agacacgagt atataagcga aagcaagatg ggtaaacgtc
    14221 tgaactgtaa gtgatcgtca gggctaatct gaaaagcccc cgtaaatcaa tcggctaaat
    14281 tgccatattt tatcatcaat cttgccgcct gatcttaatc tgaaaaatca tccaatacag
    14341 acagataccc attacacctc gggtcataac cagcaggatc aaccgcatgt aaattaaaaa
    14401 agagcgtgtt gttctgatga aacaaaaaga gctatggatt aaccagatca aagggttatg
    14461 tatttgtctg gtggtgattt atcactcggt cattaccttt tatccgcata tgaccacttt
    14521 tcagcatccg ttatcggaag tcctgagcaa atgctggatc tatttcaatc tttaccttgc
    14581 cccctttcgt atgccggttt ttttctttat atccggctat ttgattcgcc gctatatcga
    14641 cagcgtaccg tggggaaatt gtctcgataa acggatctgg aacatcttct gggtgctggc
    14701 gctttggggc gtggtgcagt ggctggcgtt aagtgcgctg aaccagtggc tggcacctga
    14761 gcgcgattta agcaatgcct ccaatgccgc ttatgccgat tccaccggtg agttcctgca
    14821 cgggatgatc accgccagca ccagcttgtg gtatctgtat gcgttaattg tctatttcgt
    14881 ggtatgtaaa atttttagcc gtctggcgct gccactattc gccttgtttg tactgctgag
    14941 tgtggcggtt aatttcgttc ccacgccgtg gtggggaatg aacagtgtga tacgcaattt
    15001 gccttattac agccttggcg catggtttgg cgcaacaata atgacctgtg ttaaagaggt
    15061 gccgttgcgc cgccatctgc tgatggcttc tttgcttacc gttctggcgg tcggtgcctg
    15121 gttgtttact atctcgctgc tgttgtcgct ggtatcgatt gtggtgatca tgaagctgtt
    15181 ttatcagtac gagcaacgat tcggtatgcg ctccaccagc ctgctgaatg tgattggctc
    15241 caacaccatt gctatctaca ccacccatcg cattctggtt gaaatattca gcttaactct
    15301 gcttgcgcaa atgaacgcag cacgctggtc gccgcaagtc gaactgacac tcctgctggt
    15361 ttatcccttt gtcagtttgt tcatctgtac tgttgcgggc ttgctggtaa gaaaactttc
    15421 acagcgtgca ttcagcgatc tgttgttctc cccgccttca ctgcccgctg ccgtcagtta
    15481 ctctcgttaa accatcaccc cagccggggc gattcaattt gctaaaccat gtcgattacg
    15541 gataatgaac agcattgctt atcagagata tgcccccata tgttgaggca tatcctaacg
    15601 agaatctgac aaccgttatg cctgaacaat atcgttatac gctgcccgtc aaagcgggtg
    15661 agcagcgtct gctgggcgag ttaaccggcg cagcctgtgc aacgctggta gcggaaattg
    15721 ccgaacgtca cgccggtccg gtggtactca ttgcaccaga tatgcaaaat gctctgcgtt
    15781 tgcatgatga aatcagccag ttcaccgatc agatggtgat gaatctggcg gactgggaaa
    15841 ctcttcccta cgacagtttt tcgcctcatc aggacattat ctcctcgcgc ctttccaccc
    15901 tttaccagct accgacgatg cagcgtggca tactgattgt tccggtgaat acgcttatgc
    15961 agcgcgtttg cccacacagt tttctccacg gtcatgcgct ggtgatgaaa aaaggtcagc
    16021 gcctgtcacg agatgcatta cgaacccaac tggacagcgc cggttatcgc catgttgacc
    16081 aggtgatgga gcacggcgaa tacgccacgc gcggcgcgtt gctggatctc ttcccgatgg
    16141 ggagtgagct gccttatcgt cttgatttct ttgatgatga aatcgacagc ctgcgggtgt
    16201 ttgacgtcga cagccagcgc acgctggagg aagtagaagc gatcaatctg ctgcccgcgc
    16261 acgaatttcc gaccgataaa gcggcaattg aactgttccg cagccagtgg cgcgatacct
    16321 tcgaagtgaa gcgcgatccg gaacatattt accagcaagt gagtaaaggc acattacctg
    16381 ctgggatcga gtactggcag ccattgttct tcagcgaacc actgccaccg ctgttcagtt
    16441 atttccctgc caataccttg ctcgtgaata ctggcgatct ggaaaccagt gccgaacgtt
    16501 tccaggctga cacgctggcg cgttttgaga atcgcggcgt cgatccgatg cgcccgctgt
    16561 tgccaccaca atcgctctgg ctgcgggtgg acgagctctt ctcagagctg aaaaactggc
    16621 cccgagtgca gctaaaaact gaacatttac cgacaaaagc cgcgaatgcc aatttaggtt
    16681 tccagaaact gccagacctg gccgttcagg cgcaacaaaa agcgccgctg gatgcgctgc
    16741 gtaagttcct cgagactttc gacggtccgg tggtgttctc ggtagaaagt gaaggtcgcc
    16801 gtgaagcgct gggtgaactg ctcgcacgaa ttaaaattgc tccgcaacgc attatgcgtc
    16861 ttgatgaagc cagcgaccgt gggcgttatc tgatgattgg cgctgccgaa catggttttg
    16921 tcgataaggc gcgtaatctg gcgctgatct gcgaaagcga tctgctcggt gaacgtgttg
    16981 cccgccgtcg tcaggattct cgccgcacca tcaaccccga tacactgatc cgtaaccttg
    17041 cggagctgca tattggtcag ccggtggtcc atctggagca cggcgtcggt cgctatgccg
    17101 gaatgaccac gctggaagct ggcggcatta ctggcgagta cctgatgctc acctatgcca
    17161 acgacgccaa actgtatgtt ccggtgtcgt cactgcatct gattagccgt tacgcaggtg
    17221 gcgcggaaga aaacgccccg ctgcataaac ttggcggcga tgcgtggtca cgcgcgcggc
    17281 agaaagcggc ggaaaaagtg cgtgatgtgg cggcggaatt gctggatatc tacgcgcaac
    17341 gcgccgccaa agagggcttc gcgtttaaac acgatcgtga gcagtatcag ttattctgcg
    17401 acagcttccc gtttgaaacc acgccggatc aggcgcaggc cattaatgcg gtacttagcg
    17461 acatgtgtca gccgctggca atggatcgtc tggtgtgcgg cgatgttggc tttggtaaaa
    17521 cagaagtggc gatgcgcgcc gctttcctgg cagtagataa ccacaagcag gtagcggtgt
    17581 tggtgcctac cacccttctt gcgcagcagc attacgacaa cttccgcgac cgtttcgcca
    17641 actggccggt acgtatcgaa atgatctccc gtttccgcag cgccaaagag cagacgcaaa
    17701 tccttgcgga agtggcggaa gggaaaatcg atattctgat cggtacgcac aaactgctgc
    17761 aaagtgacgt caagtttaaa gatttaggcc tgctgattgt cgatgaagaa caccgcttcg
    17821 gggtgcgtca taaagagcgc attaaagcga tgcgcgcgaa cgtggatatt ctgacgctta
    17881 ctgcaacgcc gatcccacgt acgctgaata tggcaatgag cggaatgcgt gacctgtcga
    17941 ttatcgccac gccgcccgcc cgtcgtctgg cagttaaaac ctttgtccgt gagtatgaca
    18001 gcctggtggt ccgggaggcg atcctgcgtg aaattttgcg cggaggacag gtttattatc
    18061 tctacaatga tgtggaaaac attcagaaag ccgccgaacg gctggcagaa ctggtgcctg
    18121 aagcacggat tgccatcggt cacgggcaga tgcgcgagcg cgaactggaa cgggtgatga
    18181 atgatttcca tcatcaacgt ttcaacgtgc tggtttgtac caccattatc gaaaccggga
    18241 tcgacatccc gacagccaac accattatca ttgaacgtgc ggatcacttc ggtctggcgc
    18301 agctgcacca gttacgcggt cgcgtcggac gttcacatca tcaggcatat gcatggctgc
    18361 tgacgccgca tccaaaagcg atgactaccg atgcacaaaa acgtcttgaa gcgattgcct
    18421 cgctggaaga tctcggggca ggttttgcgc tggcaacgca cgatctggag attcgcggcg
    18481 cgggtgaact gcttggcgaa gaacaaagcg gctcaatgga aaccatcggt ttctcgctgt
    18541 atatggagtt gctggaaaac gccgtcgatg cactgaaagc cggacgcgag ccgtcgctgg
    18601 aagatctcac cagccagcaa acagaagtcg agctgcggat gccgtcgcta ttgccagatg
    18661 atttcatccc tgacgtgaac acgcgtctgt cgttctacaa acgtattgcc agcgccaaaa
    18721 cggaaaacga actggaagag atcaaagtcg agcttatcga tcgcttcggc ctgctgccag
    18781 atccggcgcg taccctgctg gatattgccc gtctgcgcca gcaagcgcag aaactgggga
    18841 tcaggaagct ggaaggtaat gagaaaggcg gggtgatcga atttgccgag aagaatcacg
    18901 ttaatccggc ctggttgatt ggtttgctgc aaaaacagcc gcagcattac cgccttgatg
    18961 gtccgacgcg cctgaaattt attcaggatt tgagtgagcg gaaaacgcgt atcgaatggg
    19021 tacgccagtt tatgcgtgaa ctggaagaga acgcgatcgc ttaatgtaaa tcgggggcgt
    19081 ttctgcgccc ccatctgcaa catttacaaa ttctttgcac tttcctgcac tatccgacac
    19141 tgtcaccatc cataattcag gcttatctgt ttattacaat aaccttatat ttattatgta
    19201 tttttggtga tgatcaaaac gcgtttttct cgctggctaa cgttttttac gttcgccgct
    19261 gccgtggcgc tggcgctacc ggcaaaagcc aacacctggc cgctgccgcc agcgagcagt
    19321 cgtctggttg gcgaaaacaa atttcatgtg gtggaaaatg acggtggttc tctggaagcc
    19381 atcgccaaaa aatataacgt cggctttctc gctctgttac aagctaaccc cggcgttgat
    19441 ccttacgtac cgcgcgcggg cagcgtgtta acgatcccgt tgcaaaccct acttccagac
    19501 gcgccgcgcg aaggcattgt gatcaacatt gcggagctgc gtctctatta ctacccgccg
    19561 ggtaaaaatt cggtaaccgt gtatccaata ggtattggtc agttaggtgg tgacacgctg
    19621 acaccgacaa tggtgaccac cgtttcagac aaacgtgcaa acccaacctg gacgccaacg
    19681 gcaaacatcc gcgcccgtta taaagcacag ggaattgagt tgcctgcggt agtgccggct
    19741 ggaccggata acccaatggg ccatcatgcg attcgtctgg cggcctatgg cggcgtttat
    19801 ttgcttcacg gtacgaacgc cgatttcggc attggcatgc gggtaagttc tggctgtatt
    19861 cgtctgcggg atgacgatat caaaacactc tttagccagg tcaccccagg caccaaagtg
    19921 aatatcatca acactccgat aaaagtctct gccgaaccaa acggtgcgcg tctggttgaa
    19981 gtacatcagc cgctgtcaga gaagattgat gacgatccgc agctgctgcc aattacgctg
    20041 aatagcgcaa tgcaatcatt taaagatgca gcacaaactg acgctgaagt gatgcaacat
    20101 gtgatggatg tccgttccgg gatgccggtg gatgtccgcc gtcatcaagt gagcccacaa
    20161 actctgtaaa tcctggataa aaaaaatccc cgcgacacgg catgtcgcgg ggaaaaaatc
    20221 agcagtagca ggcattaatg agggttaatg cttatttata aattactgct gttccatgga
    20281 gggtattcgg accggttaca gaagtaatac ggaaagattt tgcgcccatc tcatccgctt
    20341 tttgcgccag ctgctcttcc agcgatccca gatttgtccc cgcgttagca ctgatagtac
    20401 cgactttttg ttggccttct ggcgttgact gaacttcgac agccgcaaag ttggcaaatg
    20461 acatggagct taaaatcgcc gcagcgatga ggtttttacg tttttcataa tagtggcctt
    20521 atgcagatga atgacgtcgc aagaaattac ttaacgatcg ataactaaat gatagatgtg
    20581 atctggatca catacaagat attttttata acaatcatta attaatttaa aaatctttaa
    20641 atttcagtca aatagaaagc aattattttt atgggatctg gactggtgaa cggaggtgat
    20701 tatttttata ataacctttc attcacagtc accaggtaca acggcatcat ggcaactgac
    20761 tcaacacaat gtgtaaaaaa aagccgtggc cgcccaaaag tgttcgacag ggatgccgcg
    20821 cttgataagg ccatgaaatt gttctggcaa cacggttatg aagcgacttc tcttgcggac
    20881 ctcgtcgaag caaccggagc caaagcgccc acgctatacg cggaatttac caacaaagag
    20941 gggttatttc gtgccgttct cgaccgctat atcgatcgtt ttgccgctaa gcatgaagca
    21001 cagctgtttt gtgaagagaa aagcgtggag tctgcgctgg ctgactattt tgctgccatc
    21061 gccaactgct ttaccagcaa agacaccccg gcaggctgct tcatgatcaa caactgcacc
    21121 accctctccc cagattcagg agatatagcc aatacgttga aatcacgcca tgcgatgcaa
    21181 gagcgcactt tgcagcagtt tttatgtcaa cgacaagcgc gcggggaaat cccgacccac
    21241 tgtgacgtga cacatctggc agaattcctt aattgtatta ttcaggggat gtcgatcagc
    21301 gcacgcgaag gtgcatcgct ggaaaaactg atgcagattg ccggaacgac tttgcgttta
    21361 tggcccgaac tggtgaaata agtcgcaaaa aaggcccctc agcctgagcg aatgaggggc
    21421 caaattgcag agtacagcct tgttatactt tgttattcaa aatcagttgc ccgttactat
    21481 ctagcgggat ctgcgtaccc ggatcgcggt ccatgcggat tttgccctgc tgatcgccaa
    21541 tcttataggt cacatcataa ccgagcattt tttctgactt gtcatacacc gttttacaac
    21601 gctgttgcgt agtcgtgtaa gtatcgcttt cctggagaga gccctggatc tggttaccgg
    21661 catatccacc acccagcgcc cccacaacag tggcgacatc tttaccgcga ccaccaccaa
    21721 actgatgccc tatcacgcca ccagcaacag cgccgagcac cgacccggta atgcgatttt
    21781 catcctgcac cggtcgacga tgggtcactg tgacgttgcg acactcctga cgcggtgttt
    21841 taaccgtttc cttgattggg gttgcagaaa caacctgagc gtattgcggg ccccgttcaa
    21901 acacgttcag actggccact gccgctacgc ccaacgcagc tgcgacacca atcccgatac
    21961 ccgccaacat tgatttattc acgggaacac ctccttcttt gcagttatgc caatcatgca
    22021 agcgaaggag aaaagcagct atcagacaga agatgaaaag cgcgggaatg actggaaata
    22081 cttaaggatt cggaggagtc tgaaggaatg cctgatgcga cgcttaacgc gtcttatcag
    22141 gcctaccgct cgtaccattt gtccagggcg gataaggcgt ttataccaca tccgccagtg
    22201 tacgtcgatt aatgcaactt caaacgcgga cggataacgc ggttaatact ccccaccagc
    22261 atcattaatc cggttttaaa gtaaccatgc agcgcaatct gatgcattcg gtatagcgag
    22321 atatatacaa agcgcgcaat tcgtccttca atcatcattg agccgcgcgt caggttaccc
    22381 atcaggctac caacggtgga gaagttcgac agcgatacca gcgaaccatg atctttatac
    22441 tgataatttt tcagcggctt accgttcatc tgcgccagaa tgttgttcat tgcgcaagtc
    22501 gccatctggt gtgcagcctg agcacgcggc ggaacaaagc ccccttccgg acgcgggcat
    22561 gacgcgcagt cgccaatagc gtaaatgtct ggatcgcgag tggtttgcag cgtcggttcc
    22621 accaccagct ggttgatacg gttagtttca agaccaccga tatctttcag gaagtctggc
    22681 gctttgatcc cggctgccca taccatcaga tcagcctcaa tatattcgcc atctttagtg
    22741 tgcaggccgc cttcatcagc actggtgacc atggtttgcg tcagcacgcg aacgccaagt
    22801 ttcgttagct cgttgtgggc cgcagcagag atacgtggcg gtaatgcagg caaaatacgt
    22861 tctcccgctt ctaccagcgt tacgttcagg gcttcgttgg tcaggccttt gtaaccgtag
    22921 ctgtgcagtt gcttgatcgc gttgtgcaat tcagcggaga gttctacacc cgtcgcgccg
    22981 ccgccgacaa tcgcaatgtt cactttgcca tttgcgccca ggttggcaga gtatttcagg
    23041 aacagattca gcatctcctg gtggaagcga cgcgcctggt gcgggttatc gaggaaaatg
    23101 cagttctctt tgacgcctgg cgtattgaaa tcgttagagg tgctacccag cgccatgacc
    23161 agggtgtcat aggcgatttt acgttccgga accaacagtt cacctttctc gtcgcgtagt
    23221 tctgcaatag tgattgtttt cgcttcacga tcaatatcaa tgacggaacc caactggaac
    23281 tggaaaccat gattgcgcgc atgggccaga tagctcaacg catcgacgcc ttcatcaagc
    23341 gagccagtcg ccacttcgtg cagcagtggt ttccacaggt ggctgtggtt acgatcgacc
    23401 agcgtaattt tggctttttt cttacgtccc agcttatgcc ccagctgtgt tgccatttcc
    23461 agcccaccag caccgccgcc gacaatcaca atctttttca atggcgtagt caacgtgccc
    23521 cccttaaatt tattaaccaa ttgttaatta atggttatta acatagctct taattaacaa
    23581 caggttacag caatgaaaat gttcttcgct attgagaata acatgaatgg tgcattggtc
    23641 ataccaaaat tgatgttaat caagttttgt tgcttaaaag ataggcaact ttaccggggg
    23701 agatcaaaaa atagagttta ttgtttatta tttgagcaaa ttcggaaggg taatataggg
    23761 tgaagcgtat atcgagggcg tttggtcgtc ctgataagat gcgcagcatc gcatcaggca
    23821 ttgatgctcg actgtcggat gcggcgtgga cgccttatcc gacttacaga attcggagcg
    23881 catcaggtac cggcaaggtt acctgatgcg acgagggcat ttacccgagg gttttgaacg
    23941 ctttaatgcg ctgtaaatgc ggggagatat tcttgaattt gtgcgtctgc tcttcgtccc
    24001 agacaatctc gtaataatga tgcaactctt cagatgtccg ctggctgtta agtgcttcat
    24061 cattacgcga caaaataacc agacaacgat cgcgattctt ctcgcggaag ttggtcacac
    24121 acttagtggc aatatcggca tactcttccg ggcgatcaat cttgccttcc atgttctcat
    24181 aagggaacaa attagggttg aagatcacct ggcggatatc gcaaagaaaa ccaatccgtt
    24241 ccgcccagta tccgcccagg ccaacgccgc aaattagcgg acgctcgtca acgttcagtt
    24301 gcaacatttt gtccacttct ttaagcagat gctgcatatc atgtttcgga tgccgcgtac
    24361 tgtagcttat caagcgtaca tccgggtcaa taaactgcaa ttgtaagact ttctcgtggt
    24421 tacccggact gttagagtca aaaccgtgta aatagataat catcgcttcc tcacataagc
    24481 cagggttagt gacctacttt ctcttcctgc cagcgttcat gtaactgatt cagacgggtg
    24541 ctgatcgctt tccagcgagc cgagtccatc agttcctgtc gcgaaaatga acctttatga
    24601 tacaaacgtg taacacgttc tgccttgatc ggtgacagat tatctaacac gctgacggcc
    24661 cctttacgat tattgcagac caggatcata tcgcaacccg catccagtga tgcctgcccg
    24721 cgttcggcat aactgcccat aatcgcggca ccttccatcg ataaatcgtc agagaaaatc
    24781 acgccgtcaa accccagttc ctgacgcaaa acggttttca gccagtaggg agaaccgctc
    24841 gccggacgcg gatcaacatc actgtagatc acatgcgcag gcataatggc gtcgagttta
    24901 ttttcgcgga ttaaggaact gaagaccgac atatctttag cgcgaatctc cgcttgtgga
    24961 cgtggatcgc acggtgtttc tttgtgtgag tctgccgtta ctgcaccgtg tcctgggaag
    25021 tgtttcccgg tcgttttcat tccggcttca tgcataccat caataaaccg gctggcaatt
    25081 gccagggctt tttgtggatc ggcatgataa gaacgctcgc caatcgccgc gctgatatgc
    25141 ccgacatcca gcactggcgc aaagctgata tcaatatcca tagcgatcat ttcgctggcc
    25201 atcagccaac ccgcctcttg cgccagtttg ccgccctctt ccattcccaa cagcgcagca
    25261 aaggattgtg ctgccggtaa gcgggtaaaa ccttcgcgaa aacgctgcac gcgtccacct
    25321 tcctgatcta ccgccaccac cagatgattg cgcgatgctg cgcggatctg gcgcaccagt
    25381 tcacgtaact gggcaggatc atgatagtta cgcgtaaaga gaatcagccc tcccaccagc
    25441 ggatgcgcca gtatttcacg ctcttccgcg tccagttcgt aaccttcgac atccaacatt
    25501 actggaccca cactgctctc cttattgttt tattaatagc tgccgccagg tgtcatcggc
    25561 cagcctgata aattgttgat cgccggtttg tcgccagcgg tactcaaacc accctccttt
    25621 gagcatcagc agccagggaa accatcgcct gacctgacgc cataattgcg ccggataaat
    25681 cttcgcgcga gtggcatagt cattgaccaa ttgccggtgc tgttcagtat tttccaccca
    25741 caccgccgcc agttccagcg cgatatcacc atctccagca tactcccagt cgatgagttt
    25801 taaccctgat gcgctatgca ctaaatttcc ggcgtggaca tccatatgca atggacttaa
    25861 gcgtaaaggt cgtggttccc gcgctttgcg cagacgtttt aacattcgca gccaacccac
    25921 agtccgccgc gccggatcgc tttgctgcca gtacagttcc agtaagggca acagcgttat
    25981 tcgccagcca aaacgtggtt gttgatgtag ataatacagc aagcctgcca gttcgttggt
    26041 atccggcaaa tacgttttta ccgcaccggg cagatagtcg actaccatcc agtcacggag
    26101 atataaatgc ggcttcggtg caatgcttgc gggtagttgt gataaagccc gatactggcg
    26161 caagaacgcg gactgcggcg catcaggatc gtgcggctga cgcacaacaa aacgctggcc
    26221 ctgatgttca atgagaaaac taccgccact aagcccacta ttaaacgtcg tgacgggatg
    26281 aaactgcggg aaaaagcgcg acagcaattc gtcgcgcgtg atgggattat tgctgcgaaa
    26341 cggcaccttt acctgaccag ataatttcgc ccgtctgcac cagcatcagc tgcatttgta
    26401 gggtcggagc gttaacgttg ccagaggcgc aggagtacag cacgtaatga gcgccgacat
    26461 tgcgggcaat gcctatggct ttactacggg tgcctaaact gtcctgcggc gacaaaccta
    26521 actgttgctt agccatcgac agttgctggg cggaaaccag ggtaaatttc ccgttattag
    26581 ccagcgcatt tcgcagcgtt tcggtcgctt ctgcggcatt cagcgaaccg ttagtacggt
    26641 tgttaacgct atcaaccagc aggacgctac ccgcagtcac cccgtcagcc ccaagcatct
    26701 tactgaccat cggctgcatt gcgccattcc agtcataatg gcgaatatgc ggcgcaggcg
    26761 gtgccgttcg atcttcgtgc tcaattgggc ctggctgctg cgggatcgtc ggcaccgagg
    26821 gcactgtggg gacaggctgt tgtggctcgg ctggttgttc cggcgctggt ttcacttctt
    26881 ctaccggtgc aggttcacgt tgccccacac acccggcgag aaacatcgcc agcgcggtaa
    26941 tcaaggcgta gcgactcatt tttgtcatca agattcaccc ctttacaaat atagataaag
    27001 tctgactttg tgcgccccca gaaaattggc gctgccgtac agcgttaccg ccgaatgtgc
    27061 gggaatggta acgctgcgtg gcctttccag aggatgcatc tccagccctc tggcgtcata
    27121 ccagtaaaaa cgataatgaa cggtaacggg ttcttgcctt tcgttataaa tcgttgagga
    27181 tgctgaaggt tgaatatcag acgtcgaaag gacgggcttt tctgcactga tgcccgcagc
    27241 caataaagat gactccatca ccagcgattg ctcatcattc accggaattt ccggatgtga
    27301 acgacagccc accagcagta acaacaccag agacaccagc ccaaagcatc cttttctcat
    27361 cgttacagac ctttatgcgc cagcattggt cccagcggac ggccacccaa caagtgcata
    27421 tggatgtggt aaacctcttg tccgccatgg cggttggtat tcatgatcag acgatagcca
    27481 tcttcggcaa taccttcttg ctcagcaatt tttgccgcta cggtgatcat gcgtcccagc
    27541 gcctgctcat gctcagctga cacgtcgttc acagtcggaa tgagaatatt cggaatgatc
    27601 agaatatgcg ttggcgcttg cggcgaaata tcgcgaaacg ccgttaccag atcatcctgg
    27661 tagacgatat cggaggggat ctcacgacga ataattttgc tgaatatagt ttcttctgcc
    27721 acgacatttt cctttttcaa atagccatgc gttgcgccat gctacgccgc gccacactgc
    27781 gagtatagag tatggtcgag ttaaacctgc tctttcaatc ttaactcacg atttcttaag
    27841 caaaaaatcg ttttgctgcc gtatatatcg ccattattcc catttctgcc tgcgaatgcg
    27901 ctgaaaagac attctctgtt tggtttacac gacaaatatg aatgcgtata tttctcattt
    27961 gcatttgcag caaaattatt cgcacactaa atataaccgc tcatcaccct cctccggcat
    28021 gagcctgacg acaacataaa ccaagagatt tcaaatgctt tcaacacaat ttaacaggga
    28081 taatcaatat caagtcatca ccaaaccgtc actacttgcc ggttgcatag cactggcact
    28141 attaccttct gccgcttttg ctgcaccagc cactgaagaa acggtgattg ttgagggttc
    28201 agccacagct ccagatgatg gcgaaaatga ttacagcgta acgtctacct ctgcgggtac
    28261 caaaatgcag atgactcaac atgatattcc tcagtcggtc actattgtta gccagcagcg
    28321 gatggaagat cagcagttac aaacgctggg cgaagtgatg gaaaacacgc tggggatcag
    28381 caaaagtcag gcggattccg atcgtgctct ttattattcc cgcggattcc agatcgataa
    28441 ctatatggtt gatggtatcc ccacctattt tgaatcgcgc tggaatctgg gcgacgcact
    28501 ttctgatatg gcactgtttg aacgcgtaga agtagtgcgt ggcgcgacag gactcatgac
    28561 cgggacgggt aatccatctg cggcaattaa tatggttcga aaacacgcga cctgtcgtga
    28621 atttaaaggc gatgtctcgg cggaatacgg tagctggaac aaagaacggt atgtggcgga
    28681 tttacaaagc ccactcaccg aagacggtaa aatccgcgcg cgaattgtcg gcggctacca
    28741 gaataacgac tcatggctgg accgctacaa tagtgaaaag accttcttct cgggcattgt
    28801 cgatgctgat ttaggcgatc ttacgacgct ttcagccggt tacgaatatc agcgcattga
    28861 tgttaatagc ccaacctggg gcggtttacc gcgctggaat actgatggca gcagcaacag
    28921 ttacgatcgc gcacgcagta ccgcacctga ctgggcgtac aacgataaag agatcaacaa
    28981 ggtctttatg accctgaagc agcggtttgc tgatacctgg caagcgacac tgaatgccac
    29041 ccactctgaa gtcgaatttg acagcaaaat gatgtatgtc gatgcctatg taaacaaagc
    29101 ggatggtatg ctggttgggc catacagtaa ttatggacct ggctttgatc atgtcggcgg
    29161 caccggttgg aacagtggca aacgtaaagt tgatgcgctg gatttgttcg ctgacggtag
    29221 ttatgaattg tttggtcgtc agcacaatct aatgtttggt ggcagttaca gcaaacaaaa
    29281 caatcgttca gttcatgggc caacatcttc ccggatgaaa ttggcagttt ctacaacttt
    29341 aatggcaatt tcccacaagc cgactggtca tcacagagcc tggcgcagga cgataccaca
    29401 catatgaaat cgttatatgc tgccactcgt gtcacccttg ccgatccgct gcatctgatc
    29461 ctcggcgcac gttataccaa ctggcgggtt gatacgctga cttacagcat ggagaaaaac
    29521 cacaccacgc cttacgctgg tctggtgttt gacatcaatg acaactggtc gacctacgcc
    29581 agctatacct ctattttcca gccgcaaaat gatcgtgaca gttcaggcaa atatctggct
    29641 ccaatcaccg gtaacaacta cgagctgggt ctgaaatcgg actggatgaa tagccgtctg
    29701 accaccacgt tagccatctt ccgtattgag caggataatg tcgctcagtc caccggtaca
    29761 cctatccccg gcagcaacgg cgaaaccgcc tataaagcgg tggatgggac agtcagtaaa
    29821 ggggtggaat ttgaactcaa cggcgcaatt accgacaact ggcagctgac atttggcgca
    29881 acgcgctatc ttgcagagga taacgaagga aacgccgtta atcctaatct gccacgcacc
    29941 acggttaaaa tgttcaccag ctatcggttg cctgtcatgc cagagttgac agtcggcggt
    30001 ggtgttaact ggcaaaatcg cgtgtatacc gacaccgtga caccgtatgg caccttccgc
    30061 gccgagcaag gtagctacgc gctggtggat ctcttcaccc gctaccaggt gacgaaaaac
    30121 ttctcgttac aggggaacgt caataacctg ttcgacaaaa cctacgatac caacatggaa
    30181 ggttctatcg tctacggcgc accgcgtaat ttcagcatta ccggcacgta tcaattctga
    30241 ataacacctg taaaaaaggc agccatctgg ctgccttagt ctccccaacg tcttacggat
    30301 tagtggttac ggatgtactc atccatctcg gttttcaggt tatcggattt agtaccgaaa
    30361 atagcctgaa caccagaacc agcaaccact acgcccgctg cgcccagttt cttcaggcca
    30421 gcctgatcca ctttagacac atcagcaacg ctgacgcgca gacgggtaat acatgcgtcg
    30481 aggttagtaa tgttttcttt accaccaaat gcagcaacca gagccggtgc catttcgctg
    30541 gtacctgttg cttttgcatc ttcagtcgcg tcttcacgac ccggcgtttt cagatccagt
    30601 gctttaatca gcacgcggaa gatggtgtag taaacaatcg cataaccgat accgacgatc
    30661 ggtaacagcc acagtttgct gctgttacca gacagaacga tgaagtcgat cagaccgtgc
    30721 gagaacgacg taccgtcacg catccccaga agaatacaga ttgggaatgc caggcctgcc
    30781 agaatcgcgt ggatgatgta caggatcggc gcaacgaaca tgaagaagaa ctcgatcggc
    30841 tcggtgatac cggtcaggaa cgaggtcagc gccgcggaga tcataatacc gcccactttc
    30901 gcgcggtttt ctggtttagc agagtgccag atagcaattg cggcagctgg cagaccgtac
    30961 attttgaaca ggaagccacc agacagttta cccgcagtcg ggtcacccgc catataacgc
    31021 ggaatgtcgc cgtggaaaac ctgacctgct gcgttggtgt attcaccaat ctgcatctgg
    31081 aaaggtacgt tccagatgtg gtgcagacca aacggtacca ggcaacgttc gatgaaaccg
    31141 taaatgccaa acgcaactac cgggttctgg taagcagccc actgagagaa ggtctggatt
    31201 gcagaaccaa tcggcggcca aatgaaggac agcacaacgc cagtaaagat ggcagccagg
    31261 ccagaaatga tcggcacaaa gcgtttaccg gcaaagaagc caagatactc aggcagctta
    31321 atacggtaga aacggttaaa catgtacgct gcaatcgcac cggagataat ccctccgagt
    31381 acgccagtat ccgccaagtg tttagaggcg atttcttcag caggtaaatg cagtaccagt
    31441 ggcgcaacca cggccatggt tttaaccatg atgccatagg caacaactgc ggccagcgcg
    31501 gatacgccat cgttattggt aaagccgagg gcgacaccga tcgcaaaaat cagtggcatg
    31561 tttgcaaaga cggaaccgcc tgcttctgcc ataacatgcg atacaacggc gggcagccag
    31621 ctgaaattcg cggaaccgac gcccagcaga atacctgcga taggtagtac ggataccggc
    31681 agcatcagcg atttaccgac cttttgcagg ttagcaaatg cattcttaaa cataattgag
    31741 agtgctcctg agtatgggtg ctttttttac gttctcacgc gtggcaaggg gggagagcct
    31801 cgccgtgtac agggcatcta agcgcccttt atttattaca cagagtaaaa taattcagtg
    31861 ccaatttgtt tgacggctat cacgtttcaa cttcagatca ttggagaagt tgcacatttt
    31921 gacaaaacat atgtcacaat atttcaaacg cggttattca ccgcccaatt ttcggcggtg
    31981 aactttactc gttttagcca ttaattacga gctttaaaaa aactcattca acggatggat
    32041 tgaaggcggg aagcgtcgat gtgaaacaga cgggcgaagt tatcggtggt tacctgcgcc
    32101 agttcttcaa cggcaacacc tttcaacaca gccatgtatt ctgcaacgtc acgaaccatc
    32161 gcaggttgat tctcttttcc tcgatgcggt accggcgcaa ggtaaggtga gtcagtttcc
    32221 accagtaatc gatccagggg gacataacgc gcagcatcgc gcagttgctc cgcattacgg
    32281 aaggtcacaa tgccggaaaa ggagatgtaa aatccgagat ccagtaattt acccgccgtt
    32341 tctctgtcct ctgtaaaaca gtgtagtacg ccaccgcaat ccgtcacttt ttcttcgcgc
    32401 agaattgcca gcgtatcggc acgggcgtca cgggtatgga cgataaccgg cttgttcagt
    32461 tcacgaccaa tctggatatg atggatgaag gactcttgct gacgtacttt agtttccggt
    32521 gtgtaataat aatccagccc ggtttcaccc agcgctacaa caccctcttc tgccgccaga
    32581 cggcgtaaat cttctacatc gtagggatca ttctggttga gtggatgcac gccacaagaa
    32641 aatacgacgt tgtcacgttc gcctaccaga tcccgcatat gtaaataacc cggtaatgtt
    32701 gtggcgactg ccagacaaaa tttcacatcg cgtgcggcgg ctttcgccag aacgtcatcc
    32761 acgtccttat gcaaagattc ataatccaga ccatcgagat ggcagtgtga gtcgactaaa
    32821 aacatgatgt ctctcttaaa gatgaggaac cggtaacaca acgcccggtt gcaggtaatg
    32881 ctcaatacgc agtaaaagat cggtgatgag aagctcgcgg ttgatgcctg tgacagacat
    32941 taactgttca cgaatgtggc aaacatcccc cagtatagcc tgcaggcacg agggagaaag
    33001 atggtttgcc agttcggcga ccaggcccgg cacatcaaca ttggtcacct gcgcagcgcc
    33061 atgatggcgt tttagcgcat ccatcagcaa cgttgccagc cagtgtaaac gcgccggagc
    33121 ttgttcatga ttaagggccg ccagcagcga ataccagtcg cccgatggca cgctatatgc
    33181 caacgcctga cacaatgttt cacgagcctg ccagttatct ccctgaaaca acgccagtgc
    33241 cgcgccaggc gaaccggcgc ttaagcgcaa tgcggcaagt aatgcatcct gtgacattgt
    33301 cacttcgcgt gaaagccaag tcacggcgta ctgttctgga ggcggcgcaa ggtaatgtaa
    33361 ccgacaacga ctacgtaatg ttgccagtaa acgttcaggc tcgcgggtag ccaggaaaaa
    33421 ccaggtttct gctggtggct cttcaagcgt tttcagcaat gcgttagccg cggcgtcggt
    33481 cagtaaggca gcatcggtta cccagacaac tttcgcacca cctaggcgtg cgtgctcatt
    33541 cagcttttcg gtgacctcac gtaccgcatc aatgcccagc gtatttttcc ctttttcggg
    33601 agccagggtg tagtaatcgg gatgcgtgcc agcctgcatc aactgacatc cacgacagtg
    33661 accgcaactt ttgtggccct gcggttgttg gcagagtaaa taacggctca gggcgtagat
    33721 taaagcatca tcgcccatgc ccggtaacgc ctgaatgagt agcgcatggt gacctcttcc
    33781 ggcctgatag ctggctacca gtttttcgaa atcaggtcgt aaccatggat accatctcat
    33841 gcgtccaact ccttcaccca gtgggtcacg gtagtgcgga ttgcatccat cacggcctcc
    33901 agcggctggg tggcatcaat ggtatgaata cttttatctt gtgcagccag ttccagatag
    33961 cgggcgcggg tgcgattaaa gaaatcgaaa gactcttgct caatacgatc cagctcgccg
    34021 cgcgcacgcg cgcgttttaa gccaacttcc ggggtaacat cgagatagag cgttaagtct
    34081 gggcgaaaat ccccgagaac agcatcacgc agtgttgcca gcatatgttg gtcaatacca
    34141 cgtcctccgc cctgatacgc ctgagtggaa agatcgtggc gatcgccaat cacccaggtg
    34201 ccgttagcca gcgcaggttt gatgaccgtt tctaccagtt gaacgcgcgc ggcataaaac
    34261 atcagaactt cggctttatc ggtaatgact tcatcgccta ccgatttgat atccagcacc
    34321 aggcttctta acttttcggc aagttgcgta ccgccaggtt cccgagtgaa aaccatgtcg
    34381 cggataccca gttgctcgag cgtctcaacc accacattac gcgcggtagt tttgcctgcg
    34441 ccttccagcc cctcaatgac gatatactta ctgcgcattt ttttccttaa gcactttcag
    34501 ataatcctgc acagacttgt tatgactggc aagattggta ttaaacgtgt gaccaccttt
    34561 accatcggcc acaaaataga gatacggcgt ttttgccgga tgcgcagcag ccttcagcga
    34621 atccgccccc ggcgtagcta tcgcacccgg cggcaaaccg gtaatggtat aggtgttata
    34681 cgctgtcggc gtttccaggt ctgcacgaga aagtttgcca ttataacgct ctcccatccc
    34741 gtaaatcacg gttgggtcgg tctgcaggcg cataccaatg cgtaaacggt tgataaatac
    34801 cgaggcaacc tgatcgcgtt cactggcaac ggcggtttct ttttcgataa ttgatgccat
    34861 cgtcaccagc tgatttttat ctttataagg cagaccgtcc gcacgccctt cccaggcgct
    34921 atcgaccgct ttcaccattt tcttgtgaac tcgcttgagt aacgcgacat cggtggtatt
    34981 ggcggtatac atcaaggtgt ctggccagaa ccaaccttca atccactccg ggttttccag
    35041 ttcaagtgcc tgcgctacgg tggcgtactt atcgtcactc agcgtgtgct tgatatacgg
    35101 agcctcacgc aattgcttga ggtagtcgct cagacgcatc ccttctacca gtcgcagcgg
    35161 gaactgtgct tctttaccgc tttccaacaa tttcagcatc tcgcgcacgg tcatctgcgg
    35221 tgtaaagcgg taagtcccgg ctttaaagtg agaaagatcc ggttcgatac gcagcagcca
    35281 ttgaaaaacc cgtgggcgat tgatgatctt atcagcataa agctgttcac cgagcgccag
    35341 acgtccggtc cctggcttca gggtaaatat cgtctcttct ttgataagca atttgctgtc
    35401 ggcaagatgg cgaaccttcc agacgcccac accagcggcg atacccagta ccaccagcaa
    35461 taacaagata atcaataaca cttttttcat gactaattcg ggcgctcaca aagtggggct
    35521 aaatattcat ataacgttgc tgacgaaaag gtgacatcgc cacaggcacg tacgggcatc
    35581 actggcatta acgcattaca aataaccatc tcatctgcct gcaacgcctc ctccagcgag
    35641 gcttgcactt cgacaagctg ataagaggat tgtgccagta aacggataca gaattgtcgc
    35701 ataatgccgt taacacctgc ctgatccagt cgcggcgtat aaactacatt gcccttccgc
    35761 cagaacaaat tagccgcaca gcattccgta acccaccctt cgctgtcaag gaccagcgcc
    35821 tcatcagcgt ttgtctgctc aagatgagag cgaatcaata cttgctcaag ccgattaaga
    35881 tgtttaatac ctgcaagatg aggattgcgc cccagccgca ccgggcttag cgccaacgtc
    35941 atcccttcgt tacgcaaacg gtcgtaatgt gcaggataag ccgtaacgga gagaatccgc
    36001 gttgctggtc cgctgttcaa tgtgctgtac cctcgcccgc cactaccgcg actgatcacg
    36061 actttcagca caccattttg ctgttctgct gccagcgttt tcatctcttg ttcaagctga
    36121 ggccagaagt cacaggaaat catcaaccgc tgacaagcat cctgtagtcg caggatatgc
    36181 gccgataaca aactgacttt accgtcgata actctggcgg tggtaaaaca accatcacca
    36241 aactgcgttg cccgatcgct tactgccagc gattcctgct tataaccgtt aattaagaac
    36301 ataccggctc cttatcagag aattgttagt gtggcagcat gttcactacg gaacaagtcg
    36361 gaataaaagc taagaaaaaa ggcccgcaag cggacctttt atatgggtgg aaaatgacaa
    36421 cttagatctt tttaaagatc aaagaaccat tagtgccacc gaagccgaag gagttacaca
    36481 gcgtgtattc cattccgctg acctgacgtg cttcatgcgg tacgaaatcc agatcgcaac
    36541 cttcatccgg gttatccagg ttgatggttg gcggaacagc ctgatcgcgc agcgccagga
    36601 tggagtagat agattctact gcacccgccg cacctaacag gtgaccggtc atagatttcg
    36661 tggagcttac caacacacgg cttgcagctt caccgaagat ggttttcacc gcctgcgctt
    36721 cagctttatc gccagccggc gtagaagtac cgtgcgcgtt aacgtagcca atctgactcg
    36781 cttcaatgcc tgcatcacgc agagcatttg ccattgccag agctgcgcct gcgccatttt
    36841 ctggcggtga cgtcatatga taagcatcgc tgctcatacc aaagccgacg agttcagcgt
    36901 aaattttcgc accgcgtttt ttcgcgtgtt cgtactcttc aagtaccagc ataccggcac
    36961 catcgcccag tacgaagcca tcacgctctt tatcccacgg gcggctcgcc gcttgcgggt
    37021 tatcattgcg ggtagataat gcacgtgccg cgccaaaacc accaacgccc agcggcgtac
    37081 tggctttctc tgcgccacct gcaaccatca cgtcagcatc gccatacgcg ataatacgcg
    37141 cagcatggcc aatgttgtgc acgccggaag tacaggcggt cgcgatagag atgcttgggc
    37201 cacgcaggcc atacatgata gtcagatgac ctgccaccat gttcacaatc gttgacggaa
    37261 cgaagaatgg gctgatctta cgtggaccac cgttcatcag agatgtgtgg ttttcttcga
    37321 tcagtccgag gccgccaatc ccggagccaa ttgcggcacc aatgcgggtt gcgttctctt
    37381 ccgttatttc aaggccagaa tcctgcatgg cctgaacgcc agcgacaatt ccatattgaa
    37441 tgaaggcatc catcttgcgc tgttctttgc gcgagataat gtcctcacag ttaaaatcct
    37501 ttactaagcc agcaaatttc gttgcatagg cgctagtatc gaaatggtcg attaggctga
    37561 tgccactctg accggcaagc agagctttcc aggtagactc tacggtattg ccgacaggag
    37621 acaacatgcc cagtccggtc acaactacac gacgcttaga cacgtttgtc ctccaaggag
    37681 ggaaaaatga ttctagtggg acaaaaagat aaaactcagg cggtcgaacg accgcctgga
    37741 gatgttcact tacgcctggt ggccgttgat gtaatcaatg gcagcctgaa cggtggtgat
    37801 tttctcagct tcttcgtccg gaatctcagt atcaaactct tcttccagag ccattaccag
    37861 ctcaacggtg tcaagagaat ccgcgcccag gtcttcaacg aaagaagcat tgttggtaac
    37921 ttcttcctgc ttaacgccca gctgttcgcc gataattttc ttaacgcgtt cttcgatagt
    37981 gctcatactc ttaaatttcc tatcaaaact cgctttcgcg atggttttcg tagtgtataa
    38041 aatgttgaaa aatttgcaac taaatcccgg caggtcttac cacgatttta cgttattttg
    38101 aggccttacc ccccaataac gcaaatcatt ttgcactaat tattactcat aaccacgcag
    38161 actgcgcgca acatgagcat tttgtgcaaa tcgcggtcag accatgtaca tcccgccgtt
    38221 cacatgcaaa gtttcacccg tgatgtaagc tgcttcgtcg gatgccagga atgcaaccgc
    38281 gttggcgatt tcctgtgcgc cgccgaggcg acccgcagga acctgcgcca ggatacccgc
    38341 acgctggtca tcgctcagcg cacgtgtcat gtccgtttca ataaagcccg gagcaacaac
    38401 gtttacagta ataccgcgtg acgcaacttc gcgcgccagt gatttactga agccgatcaa
    38461 gcccgctttc gccgcagcgt agttggcctg accgccattt cccatggtac caaccacaga
    38521 accgatagtg ataatacgac catgacgctt tttcatcata gcgcgcatta ccgcttttga
    38581 cagacggaaa acagatgaaa ggttagtttc gataatatcg ttccactctt catctttcat
    38641 tcgcattaac aggttatcac gagtgatacc ggcattattg accaggatat ccacttcacc
    38701 aaattctgcg cgaatttttt ccagaacaga ttcgatagat gccgggtcgg tcacattcaa
    38761 catcagacct ttgccgttgg cacctaaata atcactgatc gcctgagcgc cattttcact
    38821 ggtcgcagtg ccaataactt tcgcgccacg ggctgcgagc gtttcagcaa ttgcgcggcc
    38881 aattccgcgg cttgcaccgg ttaccagtgc gatttttcct tcaaaattca tgattttcct
    38941 cttttaaagc tcgagcgccg ctgccatcgc tgaaggttcg ttcagcgccg aggcggtcag
    39001 ggtgtcgaca atgcgtttcg tcaggccagt aagcaccttg cccgggccga cttcatagag
    39061 atgttctacg ccttgcgctg ccatgtactc aacagacttc gtccactgaa ccgggttata
    39121 caactgacgt accagtgcgt cacggatggc atcaccattg gtttcgcatt tcacatcaac
    39181 gttattcaca acagaaactg ttggtgcgtt aaaggtgatt ttcgctaatt ctactgccag
    39241 tttgtcggct gctggtttca tcagcgcaca gtgagacggt acgctcactg gtaacggcag
    39301 cgcgcgtttt gcgccagcag ctttacaggc agcgccagca cgctcaaccg cttctttatg
    39361 accggcaata accacctgtc ccggagagtt aaagtttacc ggagaaacga cctgaccttc
    39421 tgcagcttct tcacacgctt tcgcaatagg cgcatcatcc agaccgatga ttgccgccat
    39481 agcgcccgtg ccttccggta cggcttcttg catgaactta ccgcgcatct caaccagacg
    39541 caccgcatca gcgaaatcaa tcacgccagc gcaaaccagc gcggagtatt cccccaggct
    39601 gtgaccggcc atcattgccg gtgctttacc gccctgctgc tgccatacgc gatacagcgc
    39661 aacagatgca gtcaacagcg ctggctgggt ttgccaggtt ttattcagtt cttcagctga
    39721 cccctgctgg gtcagcgccc acaggtcgta gcccagcgcc gcagaagctt cagcaaacgt
    39781 ttcttcgaca attggatagc tcgccgccat atcagccagc attccaacgg tttgagaacc
    39841 ctgtccaggg aacacaaatg caaattgcgt catgttttaa tccttatcct agaaacgaac
    39901 cagcgcagag ccccaagtga atccaccgcc aaaggcttca agcagaacca actgccccgg
    39961 cttaatgcgc ccgtcgcgta cagcttcatc cagcgcgcac gggacagagg ccgcagaggt
    40021 attaccgtgg cgatccagcg tcaccacgac attgtccatc gacataccga gttttttcgc
    40081 cgttgcactg ataatacgca ggttagcctg atgcggaacc agccagtcca gttgagaacg
    40141 gtcaagatta ttcgccgcca gcgtctcatc aacgatgtgc gccagttccg ttaccgcaac
    40201 cttgaagact tcgttgcccg ccatcgtcag atgaattgaa ttctctggat tcacgcggtc
    40261 ggcgtttggc agcgtcagca attcaccata actaccgtcg gcatgcagat gggtggaaat
    40321 gattcccggc tcttcagagg cagccagcac cgcagcgccc gcgccatcgc caaaaataat
    40381 aatagtccca cgatctgttg gatcgcaggt gcgcgccagt acatcggaac cgacgaccag
    40441 agcatacttc accgccccag atttcacgta ttgatcggct acgcttaatg cataggtgaa
    40501 acctgcacag gctgctgcaa cgtcaaatgc cgggcaacct ttaatgtcca acatgctttg
    40561 aatctgacaa gctgcgctcg ggaaagcgtg cgtagcagaa gtcgttgcca caacgatcag
    40621 gccaatctgg tctttctcaa tgcccgccat ctcaattgcg cgtgtcgccg cttcaaagcc
    40681 catggttgaa acggtttcgt ttggcgcggc aatgtggcgt tcgcggatac cggtacgagt
    40741 gacaatccac tcgtcagagg tgtccaccat tttttccaaa tcggcgtttg tccgcacttg
    40801 ttcgggcaga tagctgccag taccaataat cttcgtatac atgtacgctc agtcactttt
    40861 cggttatata ccgtcacttg caaactgcga gttcgctggc agcgtcctgc taccgcagag
    40921 ttccgctttt gccaccgtcc agcagctcaa aaccagctgg gtatacagat tccaggcgag
    40981 cggcaattcg ctgaggaact tgtcgctgca ccgcctgcac tgcctgttca atcgcgaccg
    41041 caaaagctcg ctgattggct gcaccatgac tttttatcac cgtgccgcgc aatcctaaca
    41101 gacaggcgcc gttatactgg tcggggttga ggtgactgaa tcgcctcgtc aggctctttt
    41161 gtagccaacg ctttaataac agtagccacc acgaccgttt tttcccttca ccctgagatt
    41221 tcagcagaga aaggaacatc ctgacaacac cttccatcgt ctttaacgtg acatttcctg
    41281 taaagccgtc acaaaccagc acatctgtct tgccagttaa caactcattg gcttcaagat
    41341 agccgatata attgatagaa gggattgttt taagcaccgc tgaggcatcc cgaatactgt
    41401 caagaccctt tacttcttct tcaccaatat tgagcaacgc cacgcgagga ttgggaattt
    41461 ccaccacctc ttcagccaga actgagccca taatggcaaa ttgcaccaac attgtgctgt
    41521 cacaatcgac gttggcccct aaatcgagga ccaccgtttt gcccttttgc tgatgtggta
    41581 ataccgtcac cagcgccgga cgctcaatcc cctccagggg cttgagtaat aattttgcca
    41641 gccccatcag tgccccggta ttaccggcac tgacacaggc ttgcgctcga ccttctttca
    41701 ccagctccag ggccatacgc attgaactcc cacggctggc gcggatagct tgcgaaggcc
    41761 gggcatcact ggcgataact gactgcgcag gaataatctg cagacgcgaa cgttgttcaa
    41821 agtcagcttt agcaagtaat ggcgtgatgg cgtcgggatt gccgactaaa agaagagtga
    41881 gttgcgaatt agaattcagt gcctgcaatg ctgcaggcac tgtcacggaa gggccaaaat
    41941 cccctcccat gacatctaac gccagggtta gacgtgtcaa ggtatcgtcg ccgcccggtt
    42001 tggtctttcc ccagttgcct ggggaaaatc ctcactaagc ttcatcacgc agacgcgtga
    42061 ttacttagcg atgaccttgc ggccgcggta gtaaccgtcg gcagtgatgt ggtgacgcag
    42121 gtgtttttca ccagaagttt tgtctacaga caggctggtg actgcggtca gcgcgtcatg
    42181 ggaacgacgc atgccacgtt tggaacgggt tggtttattc tgttgtacgg ccatggacct
    42241 tactcctcaa ttacggttta tccccgatcc aacggggagc accaattact tacgctttaa
    42301 gctggctaat acggcaaatg ggtttggctt ttgcacttct tcaggcagtt caccaaagac
    42361 catgtccgct tcggacactt cacagtgttc agaatcatgc accggaacta ccggcaaggc
    42421 gaggatgatt tcatcttcaa ccattgcaag cagatcgatt tcaccgaatt cgttaacctc
    42481 aatcggttca tacgcttccg gcagtgcttc agcctgttcg tctgaacgca caggactaaa
    42541 acaatacgtt gtgtagacct gatgagtaaa cggcttcccg caacgctgac actcgagcgt
    42601 taccgtcacc ttcgcatcgc cgtttaacac tgcgagacgt tggttatcga tagcgaacga
    42661 catggagcat tccacatcac tgtccacact gactacggat tcggcgacgc gctcaacctg
    42721 atcaggggta tagataccct ggtaatcaag gcgtttttga gccgtacgaa ccggatcgag
    42781 agtcaggggt aattttacct tttgcatagg gcgcgcatat taactttgta acgtcataga
    42841 gtcaaagaaa aaggcagcct gtggttgcct tttgccaata attcgcacac attgcgggtt
    42901 actgctttat tttcgttcag tgctgataac gaacatcaac ctgcaaaacg aagcgcatag
    42961 tttaaaatgg ctatcatcaa tacgctatat ctgatggaaa aaaatatgcc taaacttatt
    43021 ttagcctcca cctcgccatg gcgtcgcgct cttctggaaa aactgcaaat ctcttttgaa
    43081 tgtgcagcac ctgaggtcga cgaaacccca cgcagcgacg aatcaccgcg acagttggtg
    43141 cttcgactgg cacaagaaaa atcgcaatct ctggcgtcac gttatccgga tcatttaatt
    43201 attggttcag accaggtatg tgttcttgat ggcgaaatca ccggtaaacc gttaacggaa
    43261 gaaaatgccc gtctgcaatt acgcaaagcc agcggcaata tcgtcacctt ctatactgga
    43321 ctggcgttgt ttaattcggc gaatgggcat ctacaaacag aagtggagcc ttttgacgtc
    43381 catttccgtc acctaagcga ggcggagatt gataattacg tgcgtaagga gcatcccctg
    43441 cactgcgcgg gtagctttaa gagtgaagga tttggcatta cgctgtttga gcgtttagag
    43501 ggacgtgacc ctaacacgct ggttggttta ccgctaatcg cgctgtgtca gatgttacgt
    43561 cgggaaggga aaaacccgct gatgggatag ttgtttgcgc cggatgcggt tacgtcgcat
    43621 ccggcaatcg gtgcaggcgg cctgatgcgg cgtaaacgcc ttatcaggcc aaccgcccat
    43681 tatcaggctt atatgcttgt attagcgcgc gttacgcagc ttttgcaaac aacgcttcaa
    43741 accttcatcc atcggcgctt cgatacgcat cacctcaccg gtccccggat gggtaaactt
    43801 caacgctgcg gcgtgcagga acagacgatt taatcccgtg cctgcttcag tgagctgtct
    43861 gtcaaattca cggtcaccgt agcgatcgtc aaaggcaatc ggatgacccg catactgtgt
    43921 atgcacacgg atctgatgag tacgtccggt taccgggcta caacgcacca gggtggcaaa
    43981 tgcatagcgt tcttccactt taaagcgtgt ttccgacggt ttgccttcct gactgacacg
    44041 cacgatacgt tcgccgcttt gcagaatatt tttcaataac ggcgcttgaa cgctcttcac
    44101 atgcgactgc cactgaccgc gcaccagcgc caggtaatct ttttgcattc ctttttcacg
    44161 taattgctca tgcagagaac gcaacgccga gcgttttttc gctaccagca acacacctga
    44221 agtgtcccgg tcgagacgat gaaccagttc aaggaaccgc gcttccgggc gcaacgcccg
    44281 caaaccttcg ataacgccga agcttaaacc actgccgcca tgtaccgccg taccggaagg
    44341 tttattcagc accaggatat gatcatcttc atataagatg acgtccgcca gcgccgccac
    44401 cttttgcaga tgtggcgaaa ccgcctcttc ttcccgctca gcaacgcgaa ccggtgggat
    44461 acgcacctca tcacccgctt cgagtttata ttcaggctta atacgttttt tgttcacccg
    44521 cacttcacct ttacgcaaaa tacggtaaat catacttttt ggtacgcctt tcaattgggt
    44581 acgcaaaaag ttatcgatac gttgccccgc ttcgtcagcg gtgatagcaa caatttttac
    44641 ggatggagtc tctgttttca tggtgggcga ttctaattag ccaacaggat tcgcgccact
    44701 catttttcta tgcttatttt tagttttgca ccttattact tcactacgtg atcactttat
    44761 tgatggttat taaaccaatc accagcaaga agtgaaaaaa ctgtgagtaa gcgggtgata
    44821 aatggtaaaa gtcatcttgc tataacaagg cttgcagtgg aataatgagg ccgtttccgt
    44881 gtccatcctt gttaaaacaa gaaattttac ggaataaccc attttgcccg accgatcatc
    44941 cacgcagcaa tggcgtaaga cgtattgatc tttcaggcag ttagcgggct gcgggttgca
    45001 gtccttaccg gtagatggaa atatttctgg agagtaatac ccagtctgtt tctttgataa
    45061 ttgcgctgtt tttccgcatg aaaaacgggc aaccgacact ctgcgcctct ttgagctgac
    45121 gataaccgtg aggttggcga cgcgactaga cacgaggcca tcggttcaca cccggaaagg
    45181 cgttactttg cccgcagctt agtcgtcaat gtaagaataa tgagtaagtt acgatgaaaa
    45241 gaatgttaat caacgcaact cagcaggaag agttgcgcgt tgcccttgta gatgggcagc
    45301 gtctgtatga cctggatatc gaaagtccag ggcacgagca gaaaaaggca aacatctaca
    45361 aaggtaaaat cacccgcatt gaaccgagtc tggaagctgc ttttgttgat tacggcgctg
    45421 aacgtcacgg tttcctccca ctaaaagaaa ttgcccgcga atatttccct gctaactaca
    45481 gtgctcatgg tcgtcccaac attaaagatg tgttgcgtga aggtcaggaa gtcattgttc
    45541 agatcgataa agaagagcgc ggcaacaaag gcgcggcatt aaccaccttt atcagtctgg
    45601 cgggtagcta tctggttctg atgccaaata acccgcgcgc aggtggcatt tctcgccgta
    45661 tcgaaggcga tgaccgtacc gaattaaaag aagcactggc aagccttgaa ctgccggaag
    45721 gcatggggct tatcgtgcgc accgctggcg taggcaaatc tgctgaggcg ctgcaatggg
    45781 atttaagctt ccgcctgaaa cactgggaag ccatcaaaaa agccgctgaa agccgcccgg
    45841 ctccgttcct gattcatcag gagagcaacg taatcgttcg cgccttccgc gattatttac
    45901 gtcaggatat cggcgaaatc cttatcgata acccgaaagt cctcgaactg gcacgtcagc
    45961 atatcgctgc attaggtcgc ccggatttca gcagcaaaat caaactgtac accggcgaga
    46021 tcccgctgtt cagccactac cagatcgagt cgcagatcga gtccgccttc cagcgtgaag
    46081 tccgtctgcc atccggtggt tccattgtta tcgacagcac cgaagcgtta acagccatcg
    46141 acatcaactc cgcacgcgcg acccgcggcg gcgatatcga agaaactgcg tttaacacca
    46201 acctcgaagc cgccgatgag attgctcgtc agctgcgcct gcgtgacctc ggcggcctga
    46261 ttgttatcga cttcatcgac atgacgccag tacgccacca gcgtgcggta gaaaaccgtc
    46321 tgcgtgaagc ggtgcgtcag gaccgtgcgc gtattcaaat cagccatatt tctcgctttg
    46381 gcctgctgga aatgtcccgc cagcgcctga gcccatcact gggtgaatcc agccatcacg
    46441 tctgcccgcg ctgctccggt accggtaccg tgcgtgacaa cgaatcgctg tcgctctcta
    46501 ttctgcgttt gatcgaagaa gaagcgctga aagagaacac ccaggaagtt cacgccattg
    46561 ttcctgtgcc aatcgcttct tatctgctga atgaaaaacg ttctgcggtg aatgcgattg
    46621 aaacgcgtca ggacggtgtt cgctgcgtga ttgtgccaaa cgatcagatg gaaaccccgc
    46681 actaccacgt gttgcgcgtg cgcaaagggg aagaaacgcc gaccttaagc tacatgctgc
    46741 cgaagctgca tgaagaagcg atggcgctgc cgtctgaaga agagttcgct gaacgtaagc
    46801 gtcctgaaca acctgcactg gcaacctttg ccatgccgga tgtaccgcca gcgccaaccc
    46861 cagctgaacc tgccgcgact gtcgtagccc cagcaccgaa agctgcaacg gcaacaccag
    46921 cagctcctgc acaacctggg ctgttaagcc gcttcttcgg cgcactgaaa gcgctgttca
    46981 gcggtggtga agaagccaaa ccgaccgagc aaccaacacc gaaagcagaa gcgaaaccgg
    47041 aacgtcaaca ggatcgtcgc aaacctcgtc agagcaaccg ccgtgaccgt aatgagcgcc
    47101 gcgacacccg tagtgaacgt actgaaggca gcgataatcg cgaagaaaac cgtcgtaatc
    47161 gtcgccaggc acagcagcaa actgccgaga cgcgtgagag ccgtcagcag gctgaggtaa
    47221 cggaaaaagc acgtaccacc gacgagcagc aagtgccgcg tcgtgaacgt agccgccgcc
    47281 gtaacgatga taaacgtcag gcgcaacaag aagcgaaggc gctgaatgtt gaagagcaat
    47341 ctgttcagga aaccgaacag gaagaacgtg tacgtccggt tcagccgcgt cgtaaacagc
    47401 gtcagctcaa tcagaaagtg cgttacgagc aaagcgtagc cgaagaagcg gtagtcgcac
    47461 cggtggttga agaaactgcc gctgccgaac caattgttca ggaagctcca gctccacgca
    47521 cagaactggt gaaagtcccg ctgccagtcg tagcgcaaac tgcaccagaa cagcaagaag
    47581 agaacaatgc tgataaccgt gacaacggtg gcatgccgcg tcgttctcgc cgctcgcctc
    47641 gtcacctgcg cgtgagtggt cagcgtcgtc gtcgctatcg tgacgagcgt tatccaaccc
    47701 agtcgccaat gccgttgacc gtagcgtgcg cgtctccgga actggcctct ggcaaagtct
    47761 ggatccgcta tccaattgta cgtccgcaag atgtacaagt tgaagagcag cgcgaacagg
    47821 aagaagtaca agtgcagccg atggtgactg aggtccctgt cgccgccgct gtcgaacctg
    47881 ttgttagcgc gccagttgtt gaagaaatgg ctgaagtcgt agaagccccc gtaccggttg
    47941 ccgaaccgca accggaagtg gttgaaacga cgcatcctga agtgattgcc gccgcggtaa
    48001 ctgaacagcc gcaggtgatt accgagtctg atgttgccgt agcccaggaa gtcgcagaac
    48061 acgcagaacc gatggttgaa ccgcaggaag agacggcaga tgcagatatt gaagaagttg
    48121 ccgaaactgc tgaggttgtg gttgctgaac ctgaagttgt tgctcaacct gccgcgccag
    48181 tcgtcgctga agtcgcagca gaagttgaaa cggtggccgc ggttgaacca gagatcaccg
    48241 ttgagcataa ccacgctacc gcgccaatga cgcgcgctcc ggcaccggaa tatgttccgg
    48301 aggcaccgcg tcacagtgac tggcagcgcc ctacttttgc cttcgaaggt aaaggtgccg
    48361 caggtggtca tacggcaacg catcatgcct ctgccgctcc tgcgcgtccg caacctgttg
    48421 agtaataatt agctcaaagt aatcaagccc tggtaactgc cagggctttt ttatttcatg
    48481 tttgaatcaa catattaaaa ttcatcaaat ggcatccttg ctaaccaaca atgcaaaata
    48541 ggcaaaaaaa atgcccgccc cggcgatccc agagcgggca gacaaaacat acccagtttc
    48601 atgatatgtt ttaaagcgag cttatttgtt gagctggaag agcgacaatc cctgcatatc
    48661 ggtaaatgct ttatacgatg cctgcaatgc cgtttgctgc atgatgtaag atgaaatagt
    48721 tgcattccag tccacatcaa ccagatcgct catctgctgc gtttgcccta aagcgcgatc
    48781 gctacctaag gaatccagcg actccagttt gttcagctgc gtgcctaatt ccgcgcgaac
    48841 ggtaaggaca ttattcagcg agtttttcag tccacggttg gttttatcca atgccgcagc
    48901 ggcgatttct ttatccgctt cgctatccgc gaccggcgtt ttcagggctg cgatggcgct
    48961 atccagcatg gcaaaaagat tggtttcaga agcgctaccg tctggttccg ctaccgcgtt
    49021 actggtaata ctgtcgaaaa ttttgtcgcc cgtatgtcct atcaccatcg aacgcgaagc
    49081 atcgacctgt tgcttaatac tttctgttcc gccaacataa tcaccgttag cctcactaaa
    49141 cggcgcagtc tctgttttat atgtagcgcc cattaccgtc agtggtgttc gccagattca
    49201 gcaactggtc acgaagcccc tgaatatccg tagccagcga ggcccggtca tcgtcactca
    49261 aggtgccatt gctggcgtag acaatttttt cctgagcatt ctggatagca gtggtgacct
    49321 ggctaagtac actctcttcc agtgacactt tttgagtggc gaaagtacgt gccagcgtgt
    49381 actggctgtt ttgcgcctgt gcctgggaga gaacaacagc ttgtgatgca gcaatgggat
    49441 cgtctgaagg gttaacgact cgcttacccg tcgacatctg ctcgccgtac ttcatccatt
    49501 ctgcctgaga attggtgatg ccgcgcatgt tttgctggta catcatctgg gtactgaaac
    49561 gcattttatc tccccttagc gaatgttaat cagcgcatca aaaatcgcgt ttgccgtctg
    49621 cagaacctgc gcattcgcca ggtaatactg ctgaaaacgt tgcagatttc cgtactcctc
    49681 atcgagattg acaccggaaa tcgactgctg ctgattggaa agctgcgtca ccacattacc
    49741 ttgcgtggtg ctactggttt tcaacgtcgc ggttttatta ccgatatcac tcactaacga
    49801 ggcataagcg tcgttaaagg atttcgcacc gcccaccgtt ttactgttgc tttgcagatc
    49861 cagcagggcc tgaccgttgc ggttgtcgct atcacccgca tcttcttcgc tcgccatcgc
    49921 gattttcgct tcgtcggtga ttaatacatc catgttgacg atggcgtcac ttactggttt
    49981 cagcgtgaag ctgtcgttaa cggcaggcgt tcctgtaaac gtcaactcca gaccatcaaa
    50041 tgccacttta ccgttggcat ctggtgtcgc cgtaaaagtg gtattgctgg caaggcgggt
    50101 gacctgccac tgattattat cgaacgagat tttgtaatct gtcgccagta ccacggaggc
    50161 atcagttacc gtggcaccga tcgcaacgtc acctttgttt ttcgtgtttt gcagaaccgc
    50221 gggcttaccg atagtaaaga aatcttcacc agcatcgccg ttagcatcaa atccggcttt
    50281 gtgttggctg ttgaaagcct cggcaaatgc cagcgccagt tgtccaagcg tattacgcgt
    50341 ctggtccaga tcctgagaac ggaatgtcag aatgccgccc agcgacccgg tattcagtaa
    50401 tttctccggg atctcaatat tgcctgccgt cccatcaacg taagcgacag tcgtacgaga
    50461 agggtcggcg ctggaaggaa ctgccgccag ttgccgcgcc gtacttccct gaaccagtga
    50521 gtaaccattg gcatcgtgat gttataagta ccgccatcct gaacgctgac ttctacgcca
    50581 acaatctggt ttaattcgct caccagttga tcgcgttgat ccagcagatt gttaggtgac
    50641 gcccctgccc ccacgcctgt caggcgcgag atttgatcgt tcaggctggc aatttgttta
    50701 gcgtaattgt tgatctgatc aacgctggca cctatcgcga tattgacctg tttgtcctgg
    50761 tcgcgcagat attgatcggt ggttttaaac tgattcacca acccttctga tttcccaatc
    50821 agcgcctggc gcgccgccgg gtcttccgcg ttactcacca gcgtttgcag gctggtgaag
    50881 aaatcctgca tctgtgttgc cagcgaagag gtactggtgg agagcatatt gtcgattttc
    50941 gacatctgct catagcgggc ggtcaggccg ctactttgcg tctgcgcagc acgtaactgg
    51001 ttggtaataa acgtatcata ctcacgctgc acaccagaaa cgtagacgcc attgccaacc
    51061 cagccgccag cgcccaacgt gctattggcc tgcgccataa tagtggtttg gcgggtatat
    51121 ccggcaacgt tatagctgga gatattatta cttgccgtat ttaacgccgc ctgggccgcg
    51181 ttcagtccgc tcatggcgtt attgatcaag ctggacatgg aggttcctta taagccttca
    51241 attacagagt attatcggca gcgacccgcc ggacttgagt tattcagaac agattatcaa
    51301 tgttcatgct gtaggttttg ctcaccttgt cgcttatcga tttcatctgc tggatcatgt
    51361 tggtgagttt acgggcatag tgaggatcgg tagcatagcc cgcgtcctgt aggacctgcg
    51421 ccccctgttc cgcactcgcg gcggtcgtca cggcggcgta gcgcgggtta cgtgttaaca
    51481 gcccaacgta atccgacaat gcttccagat acgagctgta gacccgaaac ttcgctttta
    51541 ctttcttcgc ttcgccgttt tcatattcag tcgtggtgat ttcagtgact tgccctttcc
    51601 agttgccaga ggctttgaca ccaaacaggt tatagcttgg ctcgccgttt tcgcggcgga
    51661 tttgccgttg cccccaacca gattcaagcg cagcctgagc gaggatcaaa tgatgtggca
    51721 caccgctttg ctggcttgcc agttgggcgg gcagcgagag ttgcgcgagg aatgctctac
    51781 tgtcacccgg cagcgaatca tcgtagttac gtggtacggc cttttgcacc agctgcgaaa
    51841 gcgtctggtt ttgataacgc accacggttt cgagcgggaa tttcatcggt gctgctggcg
    51901 tggactcctc tggcaatggt tgttctggcg tcatctgttt aaccatcatc tctgcaagcc
    51961 ccagaccttt gcccgccgtc atctgttggg caatttgctg gtcatacata ctggtataca
    52021 gacgagtgtg ctcgctgctg aacaggccat cttttggtaa agcgtcgcgc atgcttttca
    52081 acatcatctg cacgaacatc ccttccacct gacgggccac cggacggata tttgccgccg
    52141 gatcttcgct cgctttcgcc tttagttcgt tgagtgattg cgcatcccag gccgcacttg
    52201 ccagtagttt gctgtcgctg atcatcagat gatttccagt tttgcccgca gacatcccgc
    52261 actttgcatt gattgcaaaa tagacatcag atccatcggc gtagcgccca gcgcattgag
    52321 cgcacgcacc acgttattga ggctggcgct ggaacgtacg ctttgcagcg aaccgccgct
    52381 ctggcgtaaa tcgatctgcg tttgtggagt aacaacagtc tgtccaccac caaacggtgt
    52441 atctggctgg ctgacattgg cctgacgatt aactgttact gagagatttc cctgcgctac
    52501 cgcgcagctg tcgagggtca cttcgcgatt catcaccacc gaaccggtgc gcgagttaat
    52561 cactacttta gcgtcctgcg gggtgacatt aacctgcata ttctggatat cggcaaggaa
    52621 acggacctgg gaactgttgc cacttggtac gcgcacctga atagtccgcg catctaacgc
    52681 ggtggcgctg ccatatccac gcacgcggtt gatggtgtca gcgatttgct gcgccatgct
    52741 gaaatcttcg tcgttaagtt gcaaattaag ggtattcccg acgccaaact ggctgggcaa
    52801 ttcacgttca ataaccgcac cattggtgat ccgtccaccg ttcagttggt taacctgaac
    52861 actgctaccg cctgcggagg ctcctgcgcc gccaaccaga atattgccct gcgccagcgc
    52921 atacacctga ctgtcaacgc ccttaagcgg agtcatcaac aacgtacctc cacgcaagct
    52981 tttggcatta cccatggaag aaaccaccac atcgatggtt tgcccctgac gtccaaacgg
    53041 aggaagtgac gctgtcacca ttaccgcagc gacgtttttt agctgcatat tggtgcccgt
    53101 cggaacggta attcccagct gtgagagcat gttattaagc gtttgtgtgg taaacggcgt
    53161 ctgggttgtc tggtcaccag tgccatccag ccccactacc aagccatagc caatcagtga
    53221 gttttgcctt accccctgaa cactggtgag atcgcgaata cgctcagcct gagccgccgt
    53281 cgtaaccagt agaagaatta atgcagagag aaatttaatc accacagcct cacttacatt
    53341 ggcgacaggt taaggaagaa acgctgcaac cagcccatat tttgcgcttc gttaatgtag
    53401 ccattgccta cgtattcaat acgcgcatcc gccacctgag tagacggtac ggtattgctg
    53461 ccgctgatag tgcgtggatt aaccacgccc gagaagcgaa taaattcggt accctgatta
    53521 atggcaatct gtttttcacc caccacatgc aggttgccgt tgaccagtac ctggtcaacc
    53581 gtcaccgtca acgtgccgct aaaggtattg ctggcattgg ccccgccctt tccgttgaac
    53641 gtgttaccac cggaggcttc gacatcggca cgagcgttac caaacagccc ctgcaaatag
    53701 cgcggcacag tatcaaagcc aaaattagtt ttaccgtcac ggctggcatt cgcagaggag
    53761 cttttgctgg cgctgacgtt ctcctgcaac acgatggtca gcgtatcgcc aatattgcgt
    53821 ggtcgacgat cttcaaacag cggttgatag ccatagttaa tcggctgagc agactggaaa
    53881 atagaaccgt tggcgacggg cgtcggaccg ggaaccggtt gtgcactggt cgccccctgc
    53941 accagcggcg tggagggtat ccaggcgcag ccggttagtg aaagcaccaa caagctggaa
    54001 atggcataag tatgcgcagc gtttttttgc atggctatca tcttcaaaaa tcagtaaacc
    54061 ggtgaacctg ccaccggtta tgccttagag ttgcgtcagt ttttgcagca tctgatcggt
    54121 ggtggacacc gctttactgt tgatttcgta agcgcgttgc acctgaatca tattgaccag
    54181 ttcttccgcc acgttgacgt tagacgtttc aacataccct tgatacagca gtcccgcgcc
    54241 gttcaggccc ggcgtgcttt cgttcggtgc accggaggat tgcgtttcgg tgtagaggtt
    54301 ttcgccaatg ctctccagtc cggtatcatt cataaaggtg gtgagattga gctgcccaac
    54361 ctgaaccgga gctgcctggc cttgttgggt tacgctgacc acgccatcac gaccgatggt
    54421 gatacttaac gcattcgccg gaatggtgat cgctggctgc acctgaaaac caccagccgt
    54481 caccagctgc ccgttctgat ccacctggaa agagccgtca cggatatagg ctgatgagcc
    54541 atctggcaac attacctgga aaaagccctg ccctttaatc gcgacatctt tgctgttatt
    54601 ggtctgcgac agattgccct ggctgtgtaa gcgttcagtg gcgaccgggc gtacccccgt
    54661 gccgatttgt aatccggagg gtaaggtggt ttgttcggaa gactgtgccc ccggctggcg
    54721 aatggtttga taaagcagat cttcaaacac cgcgcgctga cgcttaaaac cgttagtact
    54781 gacgtttgcc aggttgttgg caatgacgtc catattggtt tgctgggcgt caaggcccgt
    54841 tttggcgatc cataatgaac tgatcatata atgtcctgta gtggattgct gacaagcaga
    54901 gcagaagcac gcgacagtgt gaatttcccc tcaccctaac cctctcccca aaggggcgag
    54961 ggaatagtcc gcgcgctttt attgcaatgt catcaatcgt tcaattaact catcgacagc
    55021 agttggttgg cacggcctgc gttatcatcg acgctgctga tcaccttcat ctgcatttca
    55081 aaacgccgcg cgctggcaat catgtcgctc attgccgcaa cggcattgac gttactgcct
    55141 tccagaaccc ccgacatcac acgcaaggtt ggatctgcct gcagtaccgg ccctcgcgtg
    55201 gcctgggttt ctgcgcttaa acgaaaaatg ccgtcgtcac cgcgctgcac ttcgctgccc
    55261 gtggctttca ccagtttcag acgccctact ggcgcaactg tatttgccgg atcgcccgga
    55321 ttgagcgccg agattgtgcc atcggcagca atcgtgattt ccgccccttc cggcacagca
    55381 attggccccg cctcgcctat caccggatgc ccctgaattg tcagttgccc ggtgggatca
    55441 acctgaatgc tgccattacg cgtataccct tcgctgccgt cagcggtctg cacggccagc
    55501 catcctgctg caacgcaacg tccagcgggc gagaggtgta atccatcttg ccgggcgtca
    55561 tgtctgcacc cggtgttgac gccgttacca gcgtgcgcgt gggcagtgaa agcccttcca
    55621 ccggcactgc gcgcaaggcg tttaactgcg cgcgaaaacc gggcgtagag gcattggcca
    55681 gattactggc ggttaccgcc tgttgattca gcgtctggct ggctgctccc atcgcagtat
    55741 aaattgcgtg atccattgag ctatcccgtc agcgattagc gtaagttaac ccgcgtgttg
    55801 aggatctggt cctgggtttt gatggtctgg gcgttagact tatagttacg ctgggcaacg
    55861 atcatattga ccagttcttt actgagatcg acgttggacg cttccagcgc accgttggtc
    55921 agagtgccaa agtttcccgt cccggctgtc cccaacagcg ccacgccaga agattgcgtc
    55981 gcagaccaga cgttgtcgcc ttcggatgcc agaccttcgt tgttggcaaa gttcgccagt
    56041 acaatctgcc ccagcagttg ggtttgttcg ttggaattgt tgccgacaac cgtaccgtca
    56101 tcattgattt gataactcac cagatcgccc ggtttgtagc cattctgggt ggttgccaca
    56161 atgttgttag cgccggttgc gatattattc gccatcgcac catccactaa tgtgccatta
    56221 gcattaaatt ccagtgttgt cgctgtcttc gcaatgctgt ttggatcact gctatcctgg
    56281 gtgtagacct gccagttatt atccccggtc ttcacaaaat agacgctcat gtcatgagca
    56341 ttaccctgac tgtcgaaaac agtcaccgaa ccttttttgt tatagctatc cgcattgctg
    56401 gcgctgaatg gcgtaacagt aggaagcgga tcactggaat tcaggttgat ctgcatcgac
    56461 gccgtggtgg tagttttcgc tgccatcagg gtattcggga tcgaaatatt ggtcggattc
    56521 gccccttgct gaatagtcgg cggcgtaccg gttaccgggt aacccgtcag ctgtaaacct
    56581 tgcgtattca acaggttacg gttttcatcc agcttaaatt gtccgttacg gctgtagaac
    56641 accgaaccgt tgctgtctac cagacggaaa aaaccgttct ggctgatagc aacgtccaga
    56701 cctcgcccgg tgttggtggt cgtgccatcg gtaaagtcct gagtgatacc ggcaactttt
    56761 acccccagtc ccactttcga accggcaaac atatcggcaa aagaggccgt gcctgattta
    56821 aagccgtagg tggcggagtt ggcgatattg ttgccaataa catcgaggtt ggtggcggca
    56881 gcgtttaatc cgctaaccgc ttgagaaaag gccatgactg actcctgaaa tgtgaaggct
    56941 taaattatct gccgtacttc gtcgagggtg gtggtgccgt aagtgccgag atccagcgta
    57001 ttaccgctgt tgccgcggat cacaccctgc accagagcaa actgcagcgg ctgggcaacc
    57061 agttgtgtac caccgttact ggcgctaatc gctacattgt aagaaccgtt cggcgcagtg
    57121 ctgccatcag tcaacgaacc gtcccaggtg aaactgtgaa ctccggcggt cagttcacca
    57181 atatcaatgg tgcgcacaac cgcgccattt ttatcggtga tggtggccgt aactttgtct
    57241 gccgcctgtt gcagctcaac accaaacggc gtggtcgtgg tcacagcccc ttcttcactg
    57301 ccggttcccg caagaacagt tgtgccgggg atcatcacgc cgtgaccgat caggttactg
    57361 gcctgtaacg actggctgtt atcaatctgt ccggaaatag atccgagcgt ggtattgagt
    57421 ttttcaatcc cgctgaccgt gctgatttgt gccaattgcg acgtcagctc gttgttttcc
    57481 attggattgg tcgggtcctg gtttttcagc tgcgccacca gcaaagtcag aaaactgctt
    57541 tgtaaatctg cggcgttgct gcccgtgagc gaactactgc tggtggtact gacgccggta
    57601 tttgtcggat cggtggtggt taccgcaatg gacatagctt tctcctttat tgaccgagcg
    57661 taagggtttt cagcatcatg cttttcaccg tgttgagcac ttcaacattg gcctgatagc
    57721 tgcgtgacgc cgacatggtg ttaaccatct ccccgacgac atcaacgttc ggcattttta
    57781 tgtagccctt tgcatctgcc agcggattac ctggttcata aaccagtttg tccggggcct
    57841 gactttctta acatcggcaa cctttacgcc gcctgtcgca gcacctggtg cagcgttaac
    57901 ctggaatacc acctgttttg cccgatatgg ctgtccatca ggaccggtca cgctatcagc
    57961 atttgccaga ttactggccg ccacgttcag gcgctgggac tgggcagtta acgccgaccc
    58021 ggcgatatca aaaatattca gcagtgccat ccgttaattt ccgctctgta aaacgttcat
    58081 catgcctttg atttgcccgc tcaacgcgct aaggctcatc tggtattgca ggctgttatc
    58141 ggcaaactgg gtgcgttcgc gatccatatc gacggtatta ccgtcaagcg aaggctggtc
    58201 cggaatacgg tattgcagtt ctgcggtagg aggcgtcagc gcctgcgccg ggatgtgctg
    58261 cgttgaggtc atcgtcagtg caaccacact ggttgcatcc cgtccacgtt gcatgacttt
    58321 tttaagttca ctggcaaaat cgatatcgcg cgcctgataa ccaggggtat cggcattggc
    58381 gatgtttgct gccagcactt cctgacgctg ggcgcgcaga ttgagcgcct cttgttgaaa
    58441 acgtaaggcg gcgtcgagct tatcgagcat atctcctccg caggtatcaa aattctgcca
    58501 tcagcttaaa tgcctttacg cgcgcgttat cggcggaata aacgcaaaat gggtcgctat
    58561 ttatgccgtt gatggtcatt gcggacaggt acaattcacg ttgtagaaat ggctgggggc
    58621 gaaaatgctg acaataaaac gtagcgtggc gatcatcgcg atactgttca gtccgttaag
    58681 tacggcgagc aatctcacat cgcaattgca caactttttt agcgcccaac tcgcgggggt
    58741 aagtgatgag gttcgtgttt ctattcgtac agcgcccaat ctactaccgc catgcgagca
    58801 gccattgctt tcgatgagca ataattcccg cctgtggggc aatgtgaatg tgttggcacg
    58861 ctgcggtaac gacaaacgat atttacaggt taatgtgcag gccacaggaa attatgtggt
    58921 tgccgcgatg cctattgtgc ggggaggaaa gctggaagct ggcaatgtca aactgaaacg
    58981 tggacgcctg gataccctgc caccgcgtac ggtgctggat atcaatcaac ttgttgatgc
    59041 cgttagcctg cgcgatctat cacccgatca acctatccag ttaacccagt ttcgccaggc
    59101 atggcgggta aaagcgggac aacgcgtcaa tgtgatcgcc agcggtgatg ggtttagcgc
    59161 caacgcagaa ggtcaggcgc tgaacaatgc agccgtcgca cagaatgcgc gggtgcgcat
    59221 ggtatcggaa caggtagtca gcggcgttgt tgatgcagat gggaatattc ttataaacct
    59281 gtaagctgtt aaagattacc cgtcccttgc cgataaataa gcaacacatg ataaaagcgc
    59341 cctcaatgag gaataaacca tgagtattga tcgcacttcg cctctgaagc ctgtaagcac
    59401 cgttcaaccg cgcgaaacca ccgacgcgcc ggtaacgaac agccgggcgg caaaaacaac
    59461 cgcctccacc agcaccagtg tgacgttaag cgacgcgcaa gcaaaactga tgcaacccgg
    59521 cagcagtgat atcaatcttg aacgtgtcga agcgttaaaa ctggcgattc gtaacggtga
    59581 actaaaaatg gacaccggca aaattgccga tgcgctgatc aacgaagcgc agcaagactt
    59641 gcagagtaac tgatcgtatg acacgtcttg cagagatcct cgaccagatg tccgctgtgc
    59701 ttaacgatct caaaacggta atggatcaag agcagcaaca tctctctatg gggcagatca
    59761 acggcagcca gttgcaatgg attacagaac aaaaaagctc actgctggcg acgctggatt
    59821 acctcgaaca gttacgcaag aaagaaccca atacagcaaa tagcgttgat attagtcaac
    59881 gctggcagga aattactgtg aaaacgcagc aactacgcca aatgaatcaa cataacggct
    59941 ggttactgga aggacagatt gagcgcaatc aacaggcgct ggaaatgttg aaaccgcatc
    60001 aggaaccgac gctatatggg gcgaacggtc agacctcaac accccatcgc ggcagtaaaa
    60061 agatttcgat ctgaatatta tctccggcct gcactgcagg ccggaatgca ttgttacacc
    60121 gtccggcggg caaattcttt aactttgaag cccagtaccg ccagtgcagc gaagtacgcg
    60181 gcaatccccg ccagcacgac cgccattaaa cgcagtaagc gccagggcat ggtacccaat
    60241 gaccactccg gcatgatatg taacatacct aaaagcacgc cagacatcac cagtaccgcc
    60301 accaccagac gcaacagaaa cgccatccag ccgggttgcg gagtaaagat tttctgctta
    60361 cgcaactgcc agtaaagcag cgaagcattc agacacgccg ccagaccaat agaaagtgac
    60421 agcccggcat gtttcaacgg accaataaac gccaggttca tcaattgcgt taaaatcagc
    60481 gtaacgatgg caattttcac tggcgtttta atgtcctggc gggaataaaa gccaggagcc
    60541 aacactttca ctacaatcag gccaatcaaa cccaccgagt aggcaattaa cgcccgctgg
    60601 gtcatcagcg catcaaacgc ggtaaattta ccgtactgga acagcgaaac ggttaacgga
    60661 cctgaaagaa tgcccaacgc aaccgcactc ggcagtgcca acaggaaaca aagacgcaac
    60721 ccccagtcca tcaaacggtt gtattcatca tgattgccac tggcaaaact tttcgacagc
    60781 gacggcagca aaatggtgcc aagcgccacg cccagcacac cggagggaaa ctccattaag
    60841 cggtcggcgt aatacatcca agacaccgaa ccggaagcaa gaaacgaggc aaaaatggtg
    60901 ttgatgatta aggagatctg gctcacagag acgccaagga tcgccggtcc catctgtttc
    60961 accacgcgca ttgctccagc atcgtggaag ttaatgcgcg gcaggaccag catgccgatc
    61021 ttcttcaggt gcggtagctg atacaccagc tgcagaatgc cgccgaccgt aacagcccac
    61081 gccagcgcca gcaccggtgg gttaaagtac ggtgcggcaa acagcgcgaa accaatcatg
    61141 ctgatgttaa gcagtgttgg cgcaaacgcc ggaatcgaga agcggttcca cgtattcaga
    61201 atcgctccca ccagcgacgc cagggagatc agcaagatat agggaaaggt aatctttagt
    61261 agctggctgg tcagggcaaa tttgtcagct gtgtcagcga agcctggcgc ggtcaccatg
    61321 atcacccacg gtgcggcgag catgccagcg accgtcacaa ccgccagcgc aagtgtcagc
    61381 aggccagaaa cataagagac aaagacccgc gtggcgtctt caccctgctt acttttatat
    61441 tccgccagaa tcggtacaaa tgcctgggaa aatgcccctt cggcaaagat acggcgtaac
    61501 aagttaggaa gtttaaaagc gacgaaaaag gcgtcggttg ccatccctgc gccaaagatt
    61561 ctggcgacaa ttgcgtctcg tgcgaagcca agcacacgcg aaaacatggt catcgagctg
    61621 acggcggcca gcgattttaa taaattcatc ggtgttctaa tccggaccca cgggcaaaac
    61681 gcctgcaagg caggcgctga aagaggcgaa tagtctacct agattatggt gaattgctac
    61741 cgccagatgt tacagggttt attcactcat cgcatcgcgc cagatcttgt caacgatacg
    61801 ttgcgccagc actgcctatt cgccggcggt ttgcggaact gtctgatttt acacgcattc
    61861 aatgaagtgc cgcgcacagc cgacaaatcc acgttgctca agagtgctct gccaaccagg
    61921 aatcggttta ttcactacac cctgcccgcg ctcctcacgc cattcgcgca tatccgtaat
    61981 gtcgatgagc gcaccgtcag tcacggcctg cacggtttca cgctgacttc cggcacggcg
    62041 atgcatacaa gtggtgatct gcaaaggacc ggccgaaaaa tggtgctcgg caaacagcat
    62101 ttcgccagcg tcgttagtca gtagcgtacc gccatccaga gaggctttgc cgcccgacaa
    62161 ccacagcgcg gtatccacca catgcagata atcatccagc aacgtgaaat aaagatcgtg
    62221 tggcccgacg ctattgctac gatgtttatc cattcttagc gaggctgcgg tggcgagttg
    62281 cgtttttaac tcaccgtaga gtggtgcgaa acgacggtta aaaccgacca tcaacgtcag
    62341 ttttttccgc gccgccagtt ccaccagcct ttcagcatcg cgcagatttt ctgccagcgg
    62401 tttatcgaca cagacatgta cccccgcatt gagtaacgta ctgaccacgt caaagtggct
    62461 ggcggtgctg gagtgcacaa aaaccgcatc acaactggcg gcaaggctgg ataacgaatc
    62521 ggcataagga atgcgccagc tgtcacaaat tggcagggct ttggcgcgcg taggcgacca
    62581 ggctccttgt aacgtccagt cagacgctgc cgccagcacc ggtaaccacg ctttttgcgc
    62641 aatgccgcct aatcccacta cgccgatacg taattttttc actttaatct cccagatggg
    62701 ccagcaacga atcaagacgt tgtttcagtt ctgccacttc gatttccagg gcttcgacgc
    62761 gggtctgtaa atcaccgtca accatgttcg acatagccgt taccgccggc tgatcttcaa
    62821 cctcaccact gaaaagatgc atgtagcggc tttcgcgttt acccggttcg cgggccagac
    62881 gcaccacaaa aggaccatct tcgcgattcg ccagttgttc cagcgtcgac tccacttccg
    62941 ccatatcgct gaattcatac attcgcgcgg cgcggctgcg cagttcaccc ggcgtctggg
    63001 caccacgcaa taacaacgtg gtgattaacg ccacttctgc ggcgctcagt ttcagatcgc
    63061 caaattctga attacaaaaa cgttgctcat atttggtgac ccgattacca aaaccgctca
    63121 ctgtgcgtag ataatgacgt ttgaccagat tatccagctg ttcctgcact tcggattcac
    63181 tcagattcat gaccggttca cggttcgttt tctgattaca ggccgtgact acaccattga
    63241 ctgagagcgg gtattgctcc ggcgtggtca cctgcttttc cagcaagcaa ccaattacgc
    63301 gagcttccag ggcagtaagt tgatatttca tgagattctc cttagcggcc gggcgtccag
    63361 tctggggtag ttaatgccgt cagtacgtga tcgcgccatt gtccatcaat caacagatag
    63421 tctttcgcat agccttcttt ttcaaaaccc agtcgcgcca gtaaatcacc gctgcgttta
    63481 ttgtgcggca tataattagc cataatgcga tgaatatgtt gggtgcgctg catataacga
    63541 atggctgcgg tcagggcttc aaacatgagt cctttgccct gccatttttg cccaatcgaa
    63601 taaccgagat agcaggcatg aaaagagccg cgaacaacat tggaaaaatt ggcaacgcca
    63661 ataatctctt tttcgtccgg gtcgaataag ccaaagtaga aagctgaacc ttgtttatga
    63721 aattcgttaa tcatccctag cctggcctgc cagcctgatg gataacagtg gctttcgtcg
    63781 cgcactggct cccagggctt gaggaaatgg cgattctctg cgtaataatc cgccagacgc
    63841 caggcatcac gatcatgcac cagacgcacg accagtcggt ctgtggttaa gcgcactttt
    63901 ggcacgttac tgcgatagcc aaacattcta tacctactcc ttcccgtaac gtctttaata
    63961 cgctgtaagt attactatac ctgcgcaacc agaccctgtg aaaagtccaa aatgccattt
    64021 tctgcattat tcacatattt gataaaaact ctctatcaaa gcgacgtttt gataaaaaaa
    64081 tactgtcccc gaagtgatgg gaaaagtctt agcggcaatg cagaatagaa agtgctcatc
    64141 ttttatttcc cgggagggga aatgtcccgc gtatcgcagg cgaggaacct gggtaaatat
    64201 ttcctgctca tcgataatat gctggtcgtg ctggggttct ttgttgtctt cccgctgatc
    64261 tctatccgct tcgttgatca aatgggctgg gccgccgtca tggtcggtat tgctctcggt
    64321 ctacgccagt ttattcagca aggtctgggt attttcggcg gcgcaattgc cgaccgcttt
    64381 ggtgccaaac cgatgattgt taccggtatg ctgatgcgcg ccgccggatt cgccacaatg
    64441 ggtatcgccc acgaaccgtg gctattgtgg ttttcatgcc tgctctcggg actcggcggc
    64501 acgttgtttg atccgccgcg ttcggcgctg gtggtgaaac taatccgtcc acaacaacgt
    64561 ggtcgttttt tctcgctgtt gatgatgcag gacagtgcca gtgcggtcat tggcgcattg
    64621 ttggggagct ggctgttgca atacgacttt cgcctggtct gcgccacagg ggccgttttg
    64681 tttgtgctat gtgcggcgtt caatgcgtgg ttgttaccgg catggaaact ctccaccgta
    64741 cgcacgcccg ttcgcgaagg catgacccgc gtgatgcgtg acaagcgttt tgtcacctat
    64801 gtcctgacgc tggcgggtta ctacatgctg gctgtacaag tgatgctgat gctgccaatt
    64861 atggtcaacg acgtggctgg cgcgccctct gccgttaaat ggatgtatgc cattgaagcg
    64921 tgtctgtcgt taacgttgct ctaccctatc gcccgctgga gtgaaaagca ttttcgtctg
    64981 gaacaccggt tgatggctgg gctgttgata atgtcattaa gcatgatgcc ggtgggcatg
    65041 gtcagcggcc tacaacaact tttcaccctg atttgtctgt tttatatcgg gtcgatcatt
    65101 gccgagcctg cgcgtgaaac cttaagtgct tcgctggcgg acgcaagagc tcgcggcagc
    65161 tatatggggt ttagccgtct gggtctggcg attggcggcg ctattggtta tatcggtggt
    65221 ggctggctgt ttgacctggg caaatcggcg caccagccag agcttccgtg gatgatgctg
    65281 ggaattattg gcatcttcac tttccttgcg ctgggttggc agtttagtca gaaacgcgcc
    65341 gcgcgtcgtt tgctggaacg cgacgcctga tttgatactc ccccctgact cctccatact
    65401 gaaagtgaac tgacagccaa atggaggagt cacgtgaagc tatacattta cgatcactgc
    65461 ccttactgcc tcaaagcccg catgattttc ggcctgaaaa atatccccgt cgaattacat
    65521 gttctgctca acgacgacgc agaaacaccc acccggatgg tcggtcaaaa acaggttccc
    65581 attctgcaaa aagatgacag ccgctatatg ccagaaagca tggatatcgt tcactatgtc
    65641 gataaactcg acggcaaacc gttactgacc ggcaaacgtt cccctgccat tgaagagtgg
    65701 ctgcgcaagg tcaatggcta cgtcaacaaa ctgctgttgc cgcgttttgc caaatcggca
    65761 tttgatgagt tttctactcc cgccgcgcgc aaatatttcg tcgacaagaa agaggccagc
    65821 gcgggtaatt ttgccgacct gctggcccac tctgacggtc tgattaagaa tatcagcgat
    65881 gatttgcgtg cgctggacaa actgatcgtc aaaccgaacg ccgtgaatgg cgaactttcg
    65941 gaagatgata ttcagctatt tccgctactg cgtaatctga cgctggtagc cggaatcaac
    66001 tggccaagcc gcgttgctaa ttaccgcgat aatatggcga aacagacaca aatcaatttg
    66061 ttatcatcaa tggcgattta atcctgaccc gccgcggcag cctgtattcg cgggttgccg
    66121 ccgttttcag ttttttctgc tggcgagttt atcgctggtg acacatgctg cgttgtgtca
    66181 ggatattgac gcagatttca tgtcgaggac taccatgaac aagtttttat ttgccgctgc
    66241 attgatcgtc agtggcctgc tcgttggctg taatcaactc acccaataca ccatcaccga
    66301 acaagaaatt aaccagtcgc ttgcgaaaca taataatttc tcaaaagata tcggtttacc
    66361 cggcgtggct gacgcccata ttgttctgac aaacttgacc agccaaattg gtcgcgaaga
    66421 gccgaataag gttaccctaa ccggagacgc caatctggac atgaactccc tgttcggtag
    66481 tcagaaagcg accatgaaac tgaagctgaa agcgctgccc gtgtttgata aagaaaaagg
    66541 tgcgatcttc ctgaaagaga tggaagtggt cgatgcgacg gtacaaccgg aaaaaatgca
    66601 aacggtgatg caaacgttgc ttccctattt gaaccaggca ttacgcaatt actttaacca
    66661 gcaacctgct tacgtcctgc gcgaagatgg cagtcagggc gaagcaatgg cgaaaaaact
    66721 ggcgaaaggc attgaagtga agccaggcga aattgtcatt ccgtttacgg attaatgacg
    66781 agggcgcatt cgcgcccttt atttttcgtg caaaggaaaa cgtttccgct tatcctttgt
    66841 gtccggcaaa aacatccctt cagccggagc atagagatta atgactgcac catcccaggt
    66901 attaaagatc cgccgcccag acgactggca ccttcacctc cgcgatggcg acatgttaaa
    66961 aactgtcgtg ccgtatacca gcgaaattta tggacgggct atcgtaatgc ccaatctggc
    67021 tccgcccgtg accacagttg aggctgccgt ggcgtatcgc caacgtattc ttgacgccgt
    67081 acctgccggg cacgatttca ccccattgat gacctgttat ttaacagatt cgctggatcc
    67141 taatgagctg gagcgcggat ttaatgaagc cgtgttcacc gctgcaaaac tttacccggc
    67201 aaacgcaacc actaactcca gccacggcgt gacgtcaatt gacgcaatca tgccggtact
    67261 tgagcgcatg gaaaaaatcg gtatgccgct actggtgcat ggtgaagtga cacatgcaga
    67321 tatcgacatt tttgatcgtg aagcgcgctt tatagaaagc gtgatggaac ctctgcgcca
    67381 gcgcctgact gcgctgaaag tcgtttttga gcacatcacc accaaagatg ctgccgacta
    67441 tgtccgtgac ggaaatgaac ggctggctgc caccatcact ccgcagcatc tgatgtttaa
    67501 ccgcaaccat atgctggttg gtggcgtgcg tccgcacctg tattgtttac ccatcctcaa
    67561 acgcaatatt caccaacagg cattgcgtga actggtcgcc agcggtttta atcgagtatt
    67621 cctcggtacg gattctgcgc cacatgcacg tcatcgcaaa gagagcagtt gcggctgcgc
    67681 gggctgcttc aacgccccaa ccgcgctggg cagttacgct accgtctttg aagagatgaa
    67741 tgctctgcag cactttgaag cattctgttc tgtaaacggc ccgcagttct atggcttgcc
    67801 ggtcaacgac acattcatcg aactggtacg tgaagagcaa caggttgctg aaagcatcgc
    67861 actgactgat gacaccctgg tgccattcct cgccggggaa acggtacgct ggtccgttaa
    67921 acaataaaaa tcgtagcttc ctgttgtcat taggttattt tacctgtata aataaccagt
    67981 atattcaaca gggggctatt atgcgaattg aagtcaccat agcgaaaact tctccattgc
    68041 cagctggggc tattgacgcc ctggctggcg aactttcccg ccgtattcag tatgcgtttc
    68101 ctgataatga aggccacgta tcggtacgtt atgccgcagc gaataattta tcggttattg
    68161 gcgcaacaaa agaagataaa cagcgcatta gcgaaattct ccaggaaacg tgggaaagcg
    68221 ccgatgactg gtttgtcagc gaataatatg cagtgatttt tgtttttgcc ggatcgcccc
    68281 ggctttttta ttttacttac gaattttgag tacaaaattt ctacacttcc tcaaaataat
    68341 tgcacaatat gctaaaaaaa tatgaatgac atgctgtttt attgttcata taaatcttaa
    68401 aagcagaaat ttatctgttc ccccctttaa aaattcggtg aataccctta cttattggta
    68461 tactgaagtt gcttcagata aaaacgcatt gaacctcgaa taacgttgtc tagtaacacg
    68521 aattaggggg ccatgatgga aaaaaataat gaagtcattc agactcatcc gctcgtaggg
    68581 tgggacatca gcaccgttga tagctatgat gcgctgatgt tgcgtttgca ctaccagacc
    68641 ccaaataagt ccgagcagga agggactgaa gttggtcaga cgctctggtt aaccactgat
    68701 gttgccagac aatttatttc gatattagaa gcaggaatcg ccaaaattga atccggtgat
    68761 ttcccggtaa acgagtatcg gcgtcattaa catgacatga ccatccaccc aaaaggcacc
    68821 tcaccggtgc ctttaccatt tctgggttgt atatctgcat tcagttaatt actctgctta
    68881 aaccatgaga gcagagagaa aaaaatgaaa tacgatctca tcattattgg cagcggttcc
    68941 gtaggcgctg ccgccgggta ttatgcaacc cgcgccggtt taaacgtgct aatgaccgac
    69001 gcccatatgc caccgcatca acacggcagc caccacggcg atacgcgatt aattcgccat
    69061 gcttatggtg aaggcgaaaa gtatgtcccg ttggtcctcc gcgcgcaaac gctgtgggat
    69121 gaactttccc gccacaacga agatgatccc atttttgtac gctctggtgt cattaacctc
    69181 ggcccggctg actccgcatt tctcgccaac gtcgcccaca gcgccgaaca gtggcaactc
    69241 aacgttgaaa agctcgatgc gcaagggatt atggcccgct ggccagaaat acgcgtcccg
    69301 gacaactaca tcggcttatt tgagactgat tccggttttt tgcgcagcga actggcgatt
    69361 aaaacctgga tccaactggc gaaggaagcg ggctgtgcgc aactgttcaa ctgcccggtc
    69421 accgcaattc gtcatgacga tgatggcgta actattgaaa cggttgacgg tgagtatcag
    69481 gcgaaaaaag cgattgtctg cgcgggaaca tgggtaaaag acctgctccc ggagctgcct
    69541 gtccagcccg tacgtaaagt atttgcctgg tatcaggccg atggccgcta tagcgtgaag
    69601 aataaattcc cggcgtttac cggtgaactg cccaatggcg atcaatatta tggttttccg
    69661 gcagaaaacg acgcgttgaa gattggcaaa cataacggag gccaggttat ccattcagcg
    69721 gatgaacgtg ttccgtttgc ggaagtggcc agtgatgggt cagaagcctt cccgttcttg
    69781 cgcaatgtat tgccgggtat cggttgctgc ctgtacggcg ctgcctgcac ctatgataat
    69841 tcgcctgacg aagattttat tatcgatacc ctacccggcc acgataatac actgctcatt
    69901 accggcctga gtgggcacgg ttttaaattt gcgtcagttt taggggaaat agctgccgat
    69961 tttgcgcaag accaaaaaag cgattttgat ttgacgccat tcaggctttc ccgcttccaa
    70021 taatcatcat tacggcctcc agaatgaggc cgtttttaca ggagtattta atgcgtcggc
    70081 tgttgcacta tctcatcaat aatattcgcg agcatctgat gctttatctt tttctctggg
    70141 gattgttggc aattatggat ctgatctatg tgttttattt ttagttttcc gcactgacat
    70201 aaactgacaa atatttaatc tccctttcat ttagcgtgaa aaattgaatg agattattcc
    70261 tgcttctaca gcactcacca gacatctctg atgctgaacc cactaaactt atgaagattc
    70321 tttcattatt aattcaaaat tattgaaata aaaataaata tcattcaatt tgttattttt
    70381 gtgttgcaat tcattctccg acacgccatt ttctttctct cgcaaattcg tattggatgc
    70441 acttatgtca ttcacaaata cccctgaacg ctatggtgtt atatctgcgg ccttccattg
    70501 gctaagcgca attattgtct acggcatgtt tgctttaggt ctgtggatgg tcacgctcag
    70561 ttattacgat ggctggtatc acaaagcacc cgaactgcat aaaagcatcg gtattttgtt
    70621 aatgatgggg ctggttattc gcgttctgtg gcgtgtcata tctccaccgc cgggtccgct
    70681 gccgagttat tcgcccatga ctcgtcttgc cgcaaaagct ggacatctcg cattgtgcct
    70741 attgctgttc gccatcggca tcagcggcta tctgatctca actgccgatg gtaaaccgat
    70801 cagcgttttt ggctggtttg acgtccccgc gacccttgcc gacgctggcg cacaggcaga
    70861 ctttgccggt gccctgcatt tttggctcgc gtggagcgtc gtagtactgt ctgttatgca
    70921 cggatttatg gccctgaagc atcatttcat cgataaagac gacactctga agcgcatgct
    70981 gggaaagtcg tcatctgact atggagtata aaaaatgaaa aaaagcctgc ttggtttaac
    71041 cttcgcgtcc ctgatgttct ctgccggttc agcggttgcc gccgattaca aaattgacaa
    71101 agaaggtcag cacgcctttg ttaacttccg catacagcat cttggctata gctggttata
    71161 tggcaccttt aaagatttcg acggtacttt tacctttgat gaaaaaaatc cggctgccga
    71221 taaagtgaat gtgacaatta acaccaccag cgtcgatac
//
LOCUS       DAJTSI010000002        67671 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000002 DAJTSI010000000
VERSION     DAJTSI010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67671
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.002"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..228
                     /locus_tag="OO327_000074"
     CDS             <1..228
                     /locus_tag="OO327_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259191.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HCR6016761.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
     gene            623..1262
                     /locus_tag="OO327_000075"
                     /pseudo
     CDS             623..1262
                     /locus_tag="OO327_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            1365..1688
                     /locus_tag="OO327_000076"
     CDS             1365..1688
                     /locus_tag="OO327_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HCR6016762.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1755..3212)
                     /gene="nuoN"
                     /locus_tag="OO327_000077"
     CDS             complement(1755..3212)
                     /gene="nuoN"
                     /locus_tag="OO327_000077"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR6016763.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3219..4748)
                     /gene="nuoM"
                     /locus_tag="OO327_000078"
     CDS             complement(3219..4748)
                     /gene="nuoM"
                     /locus_tag="OO327_000078"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR6016764.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4912..6753)
                     /gene="nuoL"
                     /locus_tag="OO327_000079"
     CDS             complement(4912..6753)
                     /gene="nuoL"
                     /locus_tag="OO327_000079"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR6016765.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6750..7052)
                     /gene="nuoK"
                     /locus_tag="OO327_000080"
     CDS             complement(6750..7052)
                     /gene="nuoK"
                     /locus_tag="OO327_000080"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR6016766.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7049..7603)
                     /gene="nuoJ"
                     /locus_tag="OO327_000081"
     CDS             complement(7049..7603)
                     /gene="nuoJ"
                     /locus_tag="OO327_000081"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR6016767.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7615..8157)
                     /gene="nuoI"
                     /locus_tag="OO327_000082"
     CDS             complement(7615..8157)
                     /gene="nuoI"
                     /locus_tag="OO327_000082"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR6016768.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8172..9149)
                     /gene="nuoH"
                     /locus_tag="OO327_000083"
     CDS             complement(8172..9149)
                     /gene="nuoH"
                     /locus_tag="OO327_000083"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR6016769.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9146..11872)
                     /gene="nuoG"
                     /locus_tag="OO327_000084"
     CDS             complement(9146..11872)
                     /gene="nuoG"
                     /locus_tag="OO327_000084"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR6016770.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11925..13262)
                     /gene="nuoF"
                     /locus_tag="OO327_000085"
     CDS             complement(11925..13262)
                     /gene="nuoF"
                     /locus_tag="OO327_000085"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR6016771.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13259..13759)
                     /gene="nuoE"
                     /locus_tag="OO327_000086"
     CDS             complement(13259..13759)
                     /gene="nuoE"
                     /locus_tag="OO327_000086"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR6016772.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13762..15564)
                     /gene="nuoC"
                     /locus_tag="OO327_000087"
     CDS             complement(13762..15564)
                     /gene="nuoC"
                     /locus_tag="OO327_000087"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR6016773.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15658..16320)
                     /gene="nuoB"
                     /locus_tag="OO327_000088"
     CDS             complement(15658..16320)
                     /gene="nuoB"
                     /locus_tag="OO327_000088"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR6016774.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16336..16779)
                     /gene="nuoA"
                     /locus_tag="OO327_000089"
     CDS             complement(16336..16779)
                     /gene="nuoA"
                     /locus_tag="OO327_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR6016775.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17410..18348)
                     /gene="lrhA"
                     /locus_tag="OO327_000090"
     CDS             complement(17410..18348)
                     /gene="lrhA"
                     /locus_tag="OO327_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR6016776.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19267..20484
                     /gene="alaA"
                     /locus_tag="OO327_000091"
     CDS             19267..20484
                     /gene="alaA"
                     /locus_tag="OO327_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR6016777.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20568..21167
                     /gene="yfbR"
                     /locus_tag="OO327_000092"
     CDS             20568..21167
                     /gene="yfbR"
                     /locus_tag="OO327_000092"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR6016778.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21226..23058)
                     /locus_tag="OO327_000093"
     CDS             complement(21226..23058)
                     /locus_tag="OO327_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR6016779.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23145..23795)
                     /gene="hxpA"
                     /locus_tag="OO327_000094"
     CDS             complement(23145..23795)
                     /gene="hxpA"
                     /locus_tag="OO327_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCR6016780.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23806..24300)
                     /locus_tag="OO327_000095"
     CDS             complement(23806..24300)
                     /locus_tag="OO327_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR6016781.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24383..24838)
                     /locus_tag="OO327_000096"
     CDS             complement(24383..24838)
                     /locus_tag="OO327_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR6016782.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25176..26378
                     /gene="ackA"
                     /locus_tag="OO327_000097"
     CDS             25176..26378
                     /gene="ackA"
                     /locus_tag="OO327_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR6016783.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26453..28597
                     /gene="pta"
                     /locus_tag="OO327_000098"
     CDS             26453..28597
                     /gene="pta"
                     /locus_tag="OO327_000098"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR6016784.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28787..30307
                     /gene="yfcC"
                     /locus_tag="OO327_000099"
     CDS             28787..30307
                     /gene="yfcC"
                     /locus_tag="OO327_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR6016785.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30340..30882)
                     /gene="yfcD"
                     /locus_tag="OO327_000100"
     CDS             complement(30340..30882)
                     /gene="yfcD"
                     /locus_tag="OO327_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR6016786.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30940..31491)
                     /gene="yfcE"
                     /locus_tag="OO327_000101"
     CDS             complement(30940..31491)
                     /gene="yfcE"
                     /locus_tag="OO327_000101"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR6016787.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31547..32191)
                     /gene="yfcF"
                     /locus_tag="OO327_000102"
     CDS             complement(31547..32191)
                     /gene="yfcF"
                     /locus_tag="OO327_000102"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR6016788.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32327..32974
                     /gene="yfcG"
                     /locus_tag="OO327_000103"
     CDS             32327..32974
                     /gene="yfcG"
                     /locus_tag="OO327_000103"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR6016789.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFEAAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33031..33393
                     /gene="folX"
                     /locus_tag="OO327_000104"
     CDS             33031..33393
                     /gene="folX"
                     /locus_tag="OO327_000104"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR6016790.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33414..34307
                     /locus_tag="OO327_000105"
     CDS             33414..34307
                     /locus_tag="OO327_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR6016791.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34355..35245)
                     /gene="rpnB"
                     /locus_tag="OO327_000106"
     CDS             complement(34355..35245)
                     /gene="rpnB"
                     /locus_tag="OO327_000106"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR6016792.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35442..36215)
                     /gene="hisP"
                     /locus_tag="OO327_000107"
     CDS             complement(35442..36215)
                     /gene="hisP"
                     /locus_tag="OO327_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR6016793.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36223..36939)
                     /locus_tag="OO327_000108"
     CDS             complement(36223..36939)
                     /locus_tag="OO327_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6016794.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36936..37622)
                     /gene="hisQ"
                     /locus_tag="OO327_000109"
     CDS             complement(36936..37622)
                     /gene="hisQ"
                     /locus_tag="OO327_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR6016795.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37712..38494)
                     /gene="hisJ"
                     /locus_tag="OO327_000110"
     CDS             complement(37712..38494)
                     /gene="hisJ"
                     /locus_tag="OO327_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR6016796.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38715..39497)
                     /gene="argT"
                     /locus_tag="OO327_000111"
     CDS             complement(38715..39497)
                     /gene="argT"
                     /locus_tag="OO327_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR6016797.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39763..40332)
                     /gene="ubiX"
                     /locus_tag="OO327_000112"
     CDS             complement(39763..40332)
                     /gene="ubiX"
                     /locus_tag="OO327_000112"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR6016798.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40427..41944)
                     /gene="purF"
                     /locus_tag="OO327_000113"
     CDS             complement(40427..41944)
                     /gene="purF"
                     /locus_tag="OO327_000113"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR6016799.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41981..42469)
                     /gene="cvpA"
                     /locus_tag="OO327_000114"
     CDS             complement(41981..42469)
                     /gene="cvpA"
                     /locus_tag="OO327_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR6016800.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42728..43390)
                     /gene="dedD"
                     /locus_tag="OO327_000115"
     CDS             complement(42728..43390)
                     /gene="dedD"
                     /locus_tag="OO327_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR6016801.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43380..44648)
                     /gene="folC"
                     /locus_tag="OO327_000116"
     CDS             complement(43380..44648)
                     /gene="folC"
                     /locus_tag="OO327_000116"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR6016802.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44718..45632)
                     /gene="accD"
                     /locus_tag="OO327_000117"
     CDS             complement(44718..45632)
                     /gene="accD"
                     /locus_tag="OO327_000117"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR6016803.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45788..46447)
                     /locus_tag="OO327_000118"
     CDS             complement(45788..46447)
                     /locus_tag="OO327_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6016804.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46530..47342)
                     /gene="truA"
                     /locus_tag="OO327_000119"
     CDS             complement(46530..47342)
                     /gene="truA"
                     /locus_tag="OO327_000119"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR6016805.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47342..48355)
                     /locus_tag="OO327_000120"
     CDS             complement(47342..48355)
                     /locus_tag="OO327_000120"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6016806.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48421..49557)
                     /gene="pdxB"
                     /locus_tag="OO327_000121"
     CDS             complement(48421..49557)
                     /gene="pdxB"
                     /locus_tag="OO327_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR6016807.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49656..50651
                     /gene="flk"
                     /locus_tag="OO327_000122"
     CDS             49656..50651
                     /gene="flk"
                     /locus_tag="OO327_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR6016808.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLTAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50648..51826)
                     /locus_tag="OO327_000123"
     CDS             complement(50648..51826)
                     /locus_tag="OO327_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6016809.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52081..53322)
                     /gene="fabB"
                     /locus_tag="OO327_000124"
     CDS             complement(52081..53322)
                     /gene="fabB"
                     /locus_tag="OO327_000124"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR6016810.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            53481..55487
                     /gene="mnmC"
                     /locus_tag="OO327_000125"
     CDS             53481..55487
                     /gene="mnmC"
                     /locus_tag="OO327_000125"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR6016811.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDESRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55608..55886)
                     /locus_tag="OO327_000126"
     CDS             complement(55608..55886)
                     /locus_tag="OO327_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR6016812.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55920..56468)
                     /gene="epmC"
                     /locus_tag="OO327_000127"
     CDS             complement(55920..56468)
                     /gene="epmC"
                     /locus_tag="OO327_000127"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR6016813.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56468..57277)
                     /locus_tag="OO327_000128"
     CDS             complement(56468..57277)
                     /locus_tag="OO327_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR6016814.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57277..58101)
                     /gene="mepA"
                     /locus_tag="OO327_000129"
     CDS             complement(57277..58101)
                     /gene="mepA"
                     /locus_tag="OO327_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR6016815.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58105..59190)
                     /gene="aroC"
                     /locus_tag="OO327_000130"
     CDS             complement(58105..59190)
                     /gene="aroC"
                     /locus_tag="OO327_000130"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR6016816.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59225..60157)
                     /gene="prmB"
                     /locus_tag="OO327_000131"
     CDS             complement(59225..60157)
                     /gene="prmB"
                     /locus_tag="OO327_000131"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6016817.1"
                     /translation="MDKIFVDEAVNELQTIQDILRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            60323..60874
                     /gene="smrB"
                     /locus_tag="OO327_000132"
     CDS             60323..60874
                     /gene="smrB"
                     /locus_tag="OO327_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR6016818.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60978..61868)
                     /locus_tag="OO327_000133"
     CDS             complement(60978..61868)
                     /locus_tag="OO327_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR6016819.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61855..62379)
                     /locus_tag="OO327_000134"
     CDS             complement(61855..62379)
                     /locus_tag="OO327_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6016820.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62376..62846)
                     /locus_tag="OO327_000135"
     CDS             complement(62376..62846)
                     /locus_tag="OO327_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6016821.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62843..63391)
                     /locus_tag="OO327_000136"
     CDS             complement(62843..63391)
                     /locus_tag="OO327_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6016822.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63396..64118)
                     /locus_tag="OO327_000137"
                     /pseudo
     CDS             complement(63396..64118)
                     /locus_tag="OO327_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64138..64860)
                     /locus_tag="OO327_000138"
     CDS             complement(64138..64860)
                     /locus_tag="OO327_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6016823.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(64920..66074)
                     /gene="yfcU"
                     /locus_tag="OO327_000139"
                     /pseudo
     CDS             complement(64920..66074)
                     /gene="yfcU"
                     /locus_tag="OO327_000139"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(66023..66778)
                     /locus_tag="OO327_000140"
     CDS             complement(66023..66778)
                     /locus_tag="OO327_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6016824.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDMKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDRD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKNGSGVATMPGGRYHH"
     gene            complement(66860..67423)
                     /locus_tag="OO327_000141"
     CDS             complement(66860..67423)
                     /locus_tag="OO327_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6016825.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16924 a        18283 c        16784 g        15680 t
ORIGIN      
        1 gaaagcaatt ctgacagcgc cccctatacg ttattaaaaa actatgctga cagatttaaa
       61 aaatattcag cagaagagca gtacgaaatt gattttcaac atcgactggt aaacagaaat
      121 tgttatttag ataaatatgg cgatgcacga atcaatgctt cttatacaca attagaaata
      181 aaacactcac aacccaaaaa tagagcatcc ggcaaaagag tgagttaaca tcattgttaa
      241 tcaattcgac cagtcagtaa atatgctgac tggtcgaaca taatttgcta aaaagtcatc
      301 actgactgat gtcagtccca ccatctgcca gttcaatcta cgggtatttc cagcatatcg
      361 cggcacgcga ttgctggtct gttgatgtag tggattgtag ttgctgactt ttcttaccct
      421 cagatttctg ccccacttgt caattcaatc tgccaataaa taacataatt atatttttat
      481 tcctcacaga aattcctttt taaaaatagc gaacccactg taaaaaacac tttttgtata
      541 cctctaccaa gcgtaataaa ataaacgctc ataattcatt ataaataata atgaaatgac
      601 gccttttatt gataatttac atatgaaaaa attttccttt tgttatatta tttctgtttt
      661 cactccctct gattattttt accaaccctg ggtcactgca ttatcgccaa cgccccgaca
      721 cgtaagtcct gagcaattag aaaaaacagt tcgttatctt acacaaactg tgcatccacg
      781 taatgccggc aatattgata atctgaatag gtccgcagag tatataaaag agttctttgt
      841 cagtagcggt gccagagtta ccgcgcaggg cgtccccgat tggctattac gactctgcgc
      901 ctggaagcca gaattaccct tacccggcaa tgtcctggct ttatcccgat cggggagact
      961 ttattgctgt ggtcggcaga atgcaggata tcaacgccgt tcgtcaggta aaagcggcat
     1021 tgttgtcatc ccgggattta tctgtttatt ctatgaatgc cccagggttt attcccggta
     1081 ttgatttttc tgatcacctc aattattggc aacacgatat tcccgccgta atgattactg
     1141 acaccgcttt ttatcgtaat aaacaatacc acttgcccgg tgataccgca gacagattga
     1201 attatcagaa aatggctcag gtggtagatg gtgttataac cttgttatac aacagtaaat
     1261 aataaaaatt tctcgaggcg ggtccagaaa cagcgacatg tacattttat tttccctcgt
     1321 ctgttttaag atgcgaaaca taaaattcaa tggaggaaaa ataaatggga atgattggct
     1381 attttgcaga aattgactca gaaaagatta accaactgct tgagtctact gaaaaacctt
     1441 tgatggataa tatacatgac acgctttcag gattacgccg actggaatca ctggatcgaa
     1501 gtgagctgcg taaacagttt tccattaagc gactcaatga aatggagatt tatccgggag
     1561 tgaccttttc agaagagtta gaggggcaac tctttgcctc gatcatgctt gatatggaaa
     1621 agctgattag cgcgtatcga cgtatgctgc gacaagggaa tcatgcgctg acggttattg
     1681 ttgggtgatg gtgtcagagc gccaaaaaaa accgccgaac atgtcggcgg ctttctgact
     1741 tacaaactaa cagtttacat cagcggcatt gctaaccgca caatactaat cagcggttgt
     1801 ggccatacac ccagcaccag taccaacagt gcagaaatca gcaccacgat accgcccgcg
     1861 ctgtactgcc agtttgacgg tgcatcgcga cccggttgtt ccggtgcgtg caggtacagg
     1921 ctcaccgcca cgcgcaggta gtagtagagg ccgattgccg aaccgacaac cacggcaccc
     1981 accagccacc acaagtgtgc ctggacaccg accgccagca cgtagaactt accgataaag
     2041 cccagcgtca tcgggatacc ggccagagac agcatcatca ccgtcatcac tgccgcgagg
     2101 atcggacgat gccagaacag accgcggtaa gagaacaggg aatcagcatc cgggccacga
     2161 tacgggctgg acatcaggct gaccacgccg aacgcgccga ggctgctgaa cagataaccg
     2221 gccaggtaaa cccctaccgc ttccatcgac atctcgccgg tttgcagcgc aatcagcgcc
     2281 accagcagat agccgaggtg agagatagat gagtaaccga gcagacgttt gatgttggtc
     2341 tggctcaacg ccatcaggtt accgaagatg atggaggcaa aggcgataat cgccagtact
     2401 acgcgaatcg cttcgctgtc acccaccggt gcgtagagga acagacgcat caccacaccg
     2461 aagatagcga ttttgctcgc cgtcgccagg aaagtggaaa ccggcgcagg cgcgccctgg
     2521 tatacgtctg gcgtccacag gtggaacggc accagagaga gtttgaagcc gaggccaaca
     2581 atcatcatgc cgaaacctgc cagcaacagc ggctcgttga gcataccgtc gccgaggttt
     2641 ttacccagcg cgacaaacga caggtcgcca gactgcgcat acaccagcgc cataccgaac
     2701 agcaggaaag aagacgctgc ggcagaaagg atggtgtatt tgatactggc ttccagcgaa
     2761 cgtttctggc ggaaagcgta accgaccagg ccaaacagcg gcaaagagat cagttcaata
     2821 ccgaggaaca gagacgccag atggtttgca ttcgccagca ggatcccgcc cagcgcggca
     2881 attaacacca acaggtagaa ctcatccttg ttgtcgttat agccttcaag ccacgggtag
     2941 gcgaaagtac aggtggcgag gctcgccaac aataccagcc cggtgtaaag catggcgaaa
     3001 ccatcaacgc gcatcagcgg cgtaacgtcc atagcgcccg cctggccaac aaaccagagc
     3061 gaaaccagcg ccgcgttaag cccaataacc gagagcgtag cgttgaggaa atgattgcgt
     3121 cgccacgcaa tggagagcat cacaaccacc accgtcaagc cgacgatcag caacggtagc
     3181 agtgcgatca ggttttgtgg agttattgtc atggcgattt acggccttgt agtagtaacg
     3241 gaattaacaa accactgctg gatattgcca atcgcggagt gcgaggtatc cagaatcggc
     3301 tgcggataga agcccagcag taccagcagc accaccagca acaggatcat aaacagctca
     3361 cgcagcgaca tccctggcag ttcctggctg gcaatctggc ttttcgcttt accgaagtaa
     3421 gcgcgatgca acatcgccag cgaataaaca gatgcaaaga ccagcccaaa ggtagagata
     3481 acggtaatca ccgggacaac ctggaagctg ccgaacagaa tcataaattc gccgacgaag
     3541 ttaccggtgc caggcatccc aagcgttgcc accgcaaaga acagcgacag tgctggcagc
     3601 catttcatct tgctccacag accgcccatc atgcgcatgt cgcgggtatg gatacgttca
     3661 taaagctgac cgcagagaat aaacagaccc gccgccgaca agccgtgcgc aatcatctgg
     3721 attaccgcgc cctggtaggc caactggctg ccggtgtaga ttgcaatcag cacgaagccc
     3781 atgtgggaaa ccgaggtgta ggcgatcaga cgtttgatat cggtctgagc gaaggtcatc
     3841 cacgcaccgt agaagatgcc gataacaccc agccacatag cgattggcgc gaactctgcc
     3901 gacgcgttcg ggaacagcgg cagggagaaa cgcaacaaac cgtaagcggc ggttttcagc
     3961 aagatccccg cgaggtccac ggaaccggcg gtcggagcct gggagtgcgc atccggcagc
     4021 cagccatgca gcggaaccac cggcattttg actgcgaagg cgatgaagaa gcccagcatc
     4081 aacagatatt ccacaccact ggacattggc gtattcagca gctcttcata gttgaaggtc
     4141 cagacgccgg tcgcattgta gtgaacaaaa accagcgcca ggatggcgat caacatcacc
     4201 agaccactcg cctgggtgta aatgaagaac ttggttgccg ccgtgatacg cgttttaccg
     4261 tcagaggctt tatgccccca cagtgcgatc aggaagtaca tcggcaccag catcatttcc
     4321 cagaagaaga agaacaggaa catgtcgatg gcaaggaaca cgccgataac gccgcccagg
     4381 atccacatca ggttgaggtg gaagaagccc tgatattttt cgatctcttt ccacgaacat
     4441 agtaccgcca gcacaccgag cagaccggtc agcacgacca tcagcagcga cagaccgtca
     4501 atggcgagat gaatagagat accaaaacgc gggatccacg gcatgtcgaa ttcagactgc
     4561 cactgcggaa ttccggcgga ttgcgtcagt gaataaccgc cctgcaacca cagttgcagc
     4621 gacagcgcca gcgtcaatcc catggtgatc agcgcgatcc agcgtggcac cttgacgcca
     4681 aagcgttcgg tctgccagca caggaagccg ccaataaagg gaattaatat tagccagggt
     4741 agtaacatgg cgatctttat tccttgtaaa agtcccgtca ggaccggatt ttcaacgaat
     4801 tctcacgaca gaaaattctc ttcggactgg gggtagtgtc ggatgcgccg cgaaggcagc
     4861 ttatccgacc tacgttctta tatgcccagg ggcaatccca caatccttaa ctcaacgcag
     4921 taccatcaac agtgccagca ccacgaccgc accgatgctc atggatgcca cataccagcg
     4981 cagataaccg ttctcgctta acagcagacc tttacctgca aagcgggaaa gaaccgccgg
     5041 gatgttcatc attgagttca gcggatcgcg tttcagcaac caggcaatac ccaggaacgg
     5101 cttgacgaac actttgtcgt acagccagtc aaagccccaa gcgttgtacc accaggtact
     5161 cagcaggcgg cccggcgcac tgttggcgat ggaggtcacc agagaacgtt tacccagcca
     5221 cagccaggct gccagcagaa tgccgaccac cgcgaccacg ccagaggtaa tttccagggt
     5281 caacatgctg ccgtgcgcca gttccgtcgt ttgcggaagc acgccctgca gcggcggtac
     5341 aatcagtgcg ccaacgaagg tggaaaggat cagcagcaca atcagcggca ggctgtgagt
     5401 tacccctttc acggcgtgag cgtgaatttg ttcttttccg tggaagacga tgaaaatcat
     5461 acggaaggtg tagagcgagg tcataaacgc accgaccaga cctgccacca tcagattgat
     5521 atgaccattc gccatcgcac ccgcgaggat ctcatcctta ctgaagaagc ccgcagtgac
     5581 cagcggcagt gccgacagtg ctgcgccgcc caccaggaag cagagataaa ccagcggaat
     5641 agatttacgc agaccgccca tcttgaagat gttctgttcg tgatggcagg ccagaatgac
     5701 ggaaccggat gccaggaaca gcagcgcttt aaagaacgcg tgagtcatca agtggaaaat
     5761 cgccgcatcc catgcctgca cgccaagcgc gaggaacatg tagccaatct ggctcatggt
     5821 agagtaagcg agaacacgtt tgatgtcggt ctgtaccagc gcggcaaaac cggccagcag
     5881 cagcgtaacc gccccgacaa tacccaccag atgcagaact tccggcgtca tcaggaacag
     5941 gccgtgggta cgggcgatca ggtagacacc cgcggttacc atcgttgcgg cgtggatcag
     6001 cgcggagaca ggcgtcgggc ccgccatcgc gtcagcaagc catgtctgca acggcaactg
     6061 cgcagattta ccgaccgcac cgcccagcag catcagcgtc gcccacatca gcatgttatt
     6121 gccgtcagca aagtgcgctg gtgccagttc caccatatcg cggaagttca gggtgcccag
     6181 ttcgttgtaa agaatgaaca gtgcgaaagc gaggaaaacg tcacccacac gggtcacgac
     6241 gaacgctttc attgctgctg cgccattctt cggatcggtg taatagaaac cgatcagcag
     6301 ataggagcac aggcccacgc cttcccagcc gaggtacatc agcagcaggt tatcggcgag
     6361 aaccagaact accatgctgg cgatgaacag gttggtgtag gcgaagaagc gagagtagcc
     6421 ctcttcaccg cgcatgtacc aggaggcgta catgtgaata aggaaaccca caccagtgac
     6481 caccgagagc atggtcagcg acaggccgtc cagcaccagg ttaaaaccga tgttaaagtc
     6541 gcctaccgac atccacgtcc acagcggctg gctgtatgcc tgctcgccgt tagcgaagaa
     6601 atcaacgccg ataaaggcgg ttaccagcgc cgccaggccc acagagccaa cgccgacgat
     6661 cgccgaaacg ttttcagacc agcgcccacg ggagaacgcc agcaggacga agccaatcaa
     6721 tggcaaaata atggttaagg caagcatgtt catccgcgca tctcacttac tgaatcgatg
     6781 ttcaggttct ggcgacgacg gtgaagttgc agcagcagcg caaggccgat actcgcttct
     6841 gccgccgcga ggctgatggc gagaatgtac atcacctgac cgtcggtctg accccagtag
     6901 cttccggcga ccacaaaggc cagcgcggag gcgttaatca tgatttccag accaatcagc
     6961 ataaacagca gattgcgacg gataaccaga ccggttaagc caagaacgaa taagattgcc
     7021 gcgaggatca gtccatgttg taaggggatc atgcgtgctc ctccgttttt cttttcgcgc
     7081 tgtcgtcttt acgattgctc agcacttcac ccgcacgctc ttcacgaccg acgtggaagg
     7141 ccacaaccag acccgcgagc agcagcatag aagccagttc caccgccagt acgtaaggcc
     7201 cgaacagcgt aatacccact gctttagcac tgattggcgt accgtcgata ccctgatcgt
     7261 taacaccgag gatggcgtaa acaatcacca ccagcatgat ggccgacaaa attgccgggc
     7321 caatccacac ctgcggtttc agccactggc gttcctgttc gatttctgaa ccgcccaggt
     7381 tgagcatcat caccacgaac acgaacagca ccataatggc acccgcgtag acgataattt
     7441 ccagcgcacc ggcgaagtaa gcgcctagtg agaagaacac cccggaaatc gccagcagcg
     7501 aaataatcag gtacagcagt gcgtgtaccg gattggtatg ggtgatcact cgcaaggtcg
     7561 caagtatggc tatcaggcca cagatataaa aagcgaactc cattgcccct ctccttacgg
     7621 taacaggctc ttgacgtcga taggcttggc ttcgttctct gcttcgccct tatctttgcc
     7681 gtcgattgcc atacctgcca tccggtagaa gttatattcc gggtatttgc ccggaccgga
     7741 gatcagcaga tcctctttct cgtaaaccag atcctggcgc ttgtattccc ccatttcgaa
     7801 atccggggtt aactgaatcg ccgtggtcgg acaggcttct tcgcacagac cacagaaaat
     7861 gcagcgtgag aagttaatgc ggaaaaattc cggataccag cgaccgtctt tggtttctgc
     7921 tttttgcagc gagatacagc cgaccgggca ggctaccgca cagaggttac aggctacgca
     7981 acgctcttcg ccgtccgggt cgcgggtcag aacgatacga ccacgataac ggggcggcag
     8041 atagaccggc tcttccgggt acattcgcgt ttcgcgtttg gcgaacgcgt gcaggccgat
     8101 catccagata ctacgaacct gggtgccgaa acctactaac aattctttta aggtcatggt
     8161 cttattgccc cttattgcgc ctgccagaga atgacagccg ccgttaccag caagttgatc
     8221 agcgtcagcg gcaggcagat tttccagccg aaggacatta cccggtcata acgcggacgc
     8281 ggtaacgacg cacgaatcaa aatgaacatc atcataaaga acgcggtttt cagcgcgaac
     8341 cagatgaatg gcggtaacaa cgggccttgc cagccaccga agaacagcgt caccatcaat
     8401 gcagagatgg tcacaatccc gatgtactca cccacgaaga acagaccgaa cttcataccg
     8461 gaatattcaa tgtggtaacc atccgccagt tcctgctcgg cttccggctg gtcaaacggg
     8521 tgacggtgac acaccgccac gcccgcgatg gcaaaggtaa taaaaccaaa gaattgcggg
     8581 atgacgttcc acacatgcgc ctggctgttg acgatgtcgg tcatgttgaa tgaaccggcc
     8641 tgcgccacca cgcccatcag ggaaagcccg aggaacactt cgtagctcag ggtctgcgca
     8701 gaagcacgca tcgcacccag cagcgagtat ttgttgttac ttgaccagcc cgcaaacagc
     8761 accgcgtaaa ccgccagacc tgccatcatc aggaagaaca aaatcccgat gttcaggtcg
     8821 gcaaccaccc aacccggact gactggcaca attgcaaagg ccagcagcag cgaggtaaag
     8881 gcaatcatcg gtgccagggt aaagatgacg cgatccgaga atttcgggat ccagtcttct
     8941 ttaaagaaca ttttgatcat gtccgcaacc agctggagcg aaccgcccca gccaacacgg
     9001 ttaggtccat aacggttctg gaacagaccc agcaggcgac gttcgccaaa gctcatgaat
     9061 gccccacagg tgacaaccac cagcaggatc accaccgctt tgaggatggt cagcaggatc
     9121 tcaatcagtt ccggtgatat ccaactcatt gttgtgcctc cttgagatcc tcaagatgcg
     9181 cgccagccag caccggagca atgccggaca tacccatcgg caagcccacc tgccctgccg
     9241 tcagtccttc ggcgatttca accggcagcg tgaccgtgtt gccatcgtaa ctaaaggaga
     9301 cgcgtgtacc tgcgttcaca cccaacttcg cggcatccgc tgggttgagt ttgatgtacg
     9361 gctgcggcat acggctctgg aagaccggag cacgctgtga caattcatcg ctgccaaaca
     9421 ggtggtagta cggcgcgata cgccatttcc cgtcctgcgg ctggaagcgt gccggtacgc
     9481 tggtgaagta atccagaccg ttttcgctgg tttcaaacag acgcacgccc ggatcgccaa
     9541 agcgcagttt gccgcccact tcgtcctgga atttgttcca cgcctgcggg gagttccagc
     9601 ccggcgccca ggcaaacggc acttgcgaac ggtgcgcggt cggctggttg ttaccttcca
     9661 tcgagaaggt gaacatggtg tcaatatcct gcggctgacg cggctcatga acgctgatat
     9721 tggcgcgcat ggcggtacga ccgctgtaac ggtgcggttc acgggccagt ttctgcccac
     9781 gaatacggaa tgtcgcatcc ggcgcagcat ctttgatacc cgccagttct gggattttcg
     9841 ccacaacagc gtcaattaca tggtcgagct gcgtccagtc cacttcacgg ctcagcaagg
     9901 tgctgtgcag cgagtgcagc cagcgccagc tttccagcat gacagttttg ctgtcgtaat
     9961 aagccggatc gtaaacctgg aagaaacgtt gggcgcgacc ttcattatta attaccgtac
    10021 cgtcgctttc agcaaagctg gcagaggaaa gcaccagatg ggcgttttcc atgatcgctg
    10081 tgcgttgatg atcaaccacc atcaccagcg gcgctttagc cagcgcagca ttcacgcggg
    10141 tagcagaagc gtaacgatgc agatcgtttt ccaacaccac caccgcgtcg gcgcgtccgg
    10201 tttccagttc ggttaacgct tcttcaagcg aaccgccacc cataatgccc agccccatgc
    10261 tgttgacgga acgggcaatc atggtgatac caacgtcagc gccgcgacct ttcagggctt
    10321 tcgcgacgtt agccgcagcc tgaatcacct ctatgctacc ggcgttcgtc ccggagataa
    10381 tcaacggttt cttcgcacct gccagtgcct gcacgatgac gtcgattttg ttttgcagct
    10441 caggttcgat accgtcaacc gctggtgcgg agttatccag cgcatgggcg atggcaaaac
    10501 ctaaacgcgc ctgatcttca accggtgcgc ggtaagtcca cgccgcgata tcatccagac
    10561 gagtgtcatc aacgttggta acaaacagcg gatgcttcgc acgttgaccg atgttgagga
    10621 ttgccgcaat ctgccagtca gccactttct gtgctgcagc catttcgcgc gctttacctt
    10681 tcaccgcctg acgcactgcc agtgcgacgc gcgcgccggt ctgggtaacg tcttcgccca
    10741 gcaccagtac cgcatcgtaa gattcgattt cgcgcagagc cggagtataa atgccgcctt
    10801 cacgcagcac tttcagcgcc agttgcagac gttcctgctc accgtgagcg ataccggtgt
    10861 agaagttttc ttcgcccacc agttcacgca gcgcaaagtt gctttccacg ctggcacgcg
    10921 gagaaccaat accgatcact ttcttcgact gacgcagaat atctgccgcg ccctgcattg
    10981 cctgttcggc gttgagggta atgaaatcat cgccacgacg ctgtactggc tgacgcggac
    11041 gatccttcag gttgacgtaa ccgtaaccga aacgaccacg gtcgcagagg aagtagtggt
    11101 ttaccgtacc gttgtaacgg ttttcgatac gacgcagttc gccgtaacgt tcaccggggc
    11161 tgatgttaca gccgatggaa cattgctggc agatgctcgg cgcaaactgc atatcccatt
    11221 tacggttgta acgctcggag tgcgttttgt cggtaaatac gccggtcggg caaatttcga
    11281 ccaggttacc ggaaaattcg ctttccagcg tgccgtcttc cgggcgaccg aagtagacgt
    11341 tgtcgtgcgc gccataaacg cccagatctg taccgtcagc gtaatctttg tagtaacgca
    11401 cacagcggta gcaggcgatg cagcggttca tttcgtgaga gatgaatggc cccaaatcct
    11461 gattacggtg ggtacgtttg gtgaaacggt agcgacggaa gctgtgtccg gtcatcacgg
    11521 tcatatcctg aagatggcag ttaccgcctt cttcacatac cggacagtcg tgcgggtggt
    11581 tggtcattaa ccactcgacc acactttcac ggaactgttt cgcttcttcg tcgtcaatgg
    11641 aaataaaggt gccatcggaa gccggtgtca tacaggacat caccaggcga ccacgcgtgt
    11701 cttccgcgtt ttggtattgc ttcaccgcac actggcggca agcaccgacg cttcccagcg
    11761 ccggatgcca gcaaaagtaa ggaatatcaa ggcccagaga cagacaagct tccagcaggt
    11821 tgtccgctcc gttgacctcg tattctttgc cgtctacatg aattgtagcc attagcatgc
    11881 ttccagtttt ctcggtcagg gaccgagcgt taatcgaaat tcggttacca gcgctctttc
    11941 agcaggttcg gctgaatccc attaatcaaa tgggtattgc tgaacggctg tttgattccc
    12001 gcctcaaatt cttcgcggaa atatttgatg gcgctctgta acggctccac tgcaccaggt
    12061 gcgtgggcac agaaagtttt acccgggcct aagaatcgac acagttgctc aagtgtttcg
    12121 atatcgcccg gctggccttc gccacgctcc agcgcacgca gaattttcac gctccacggc
    12181 agaccgtcgc ggcacggcgt acaccagccg caggactcac gggcgaaaaa ctcttccagg
    12241 ttacgcacca gcgacaccat gttgatctca tggtcaaccg ccatcgccag cgccgtaccc
    12301 agacggctgc ccgctttacc gatactttcg aattccatcg gcagatcaag gtgcgcttcg
    12361 gtcaggaagt cagtccccgc gccgcctggc tgccaggctt taaatttcag accatcacgc
    12421 ataccaccgg cgtaatcttc gaggatctcg cgtgcggtgg tgccgaacgg cagttcccac
    12481 agtcccggat ttttcacccg accggagaag cccatcagct tggtgccagc atctttactt
    12541 ttcgagatgt tctgatacca ctccacgccg ttagcgagga tcgccggaac gttacacagg
    12601 gtttcgacgt tgttgacaca ggtcggtttg ccccatacgc cggaggttgc cgggaagggg
    12661 ggtttcgagc gtgggttagc acgacgccct tccagggagt tgattaacgc tgtttcttcc
    12721 ccgcagatgt agcgccctgc cccggtatgg acgaacagtt cgaagtcgaa acctgttccc
    12781 ataatgtttt tgccaagcaa gcccgcttca gtggcttcgg caatggcgcg gcgcagattt
    12841 actgccgctt cgatatattc gccacgcagg aagatgtagc cacggtaagc tttcagcgca
    12901 aacgcggaga tgagcatacc ttccaccagc aggtgcggca gttgctccat caacaggcgg
    12961 tctttatagg tgcccggctc catttcatcg gcgttacaca gcaggtaacg gatgttcatg
    13021 gattcgtctt tcggcatcag gctccacttc aggccagtcg aaaagcccgc gccgccgcgc
    13081 cctttcagac cagcgtcttt tacctgatta acgatttcgt ccggagacag cccggtcagc
    13141 gccttacgcg ccccttcgta accgtttttg ctgcggtatt cgtccagcca cactggctgt
    13201 ttgtcatcgc gcagacgcca ggtcagcgga tgcgtttcgg gagtacggat aatgtttttc
    13261 atttataccg ctccagcagt tcagggatcg cttccggggt cagatgcgcg tgagtgtcct
    13321 catcgatcat catgtttggc cctttatcac agttccccag gcagcaagtt ggcagcagcg
    13381 taaagcggcc atcaaatgtc gtttgccctg gtttgatgtt cagctttttc tcgagcgccg
    13441 cctgaatacc ctgatacccg ttgatatgac agaccacgct gtcgcaataa cggatcacat
    13501 ggcgaccaac cggctggcgg aagatctgac tgtagaacgt tgccacacct tcgacgtcgc
    13561 ttgccggaat acccagcaca tcggcgatcg cgtggatcgc accatccggc acccagccac
    13621 gctgcttctg aacgattttc agcgcttcaa tggacgccgc acgcgggtct tcgtagtggt
    13681 gcatctcgtg ctcaatcgct tcacgctctg ccgcactcag ctcaaaagcc tcggtttgtg
    13741 gttgttgatt ctcgtgcata attagcggtc cacatctgac ataacaaaat cgatactgcc
    13801 cagataaaca atcaggtcag acaccaggct gccgcggatc gccgccggaa tttgctgcaa
    13861 atgcgcataa ctcggcgtgc ggatacgagt gcgataactc atggtgctac cgtcgctggt
    13921 caggtagtaa ctgttgatcc ccttggtcgc ctcaatcatc tggaaagatt cattggcagg
    13981 catcaccggc ccccacgaca cttgcaggaa gtgggtgatc agggtttcga tatgttgcag
    14041 cgtgcgctct ttcggcggcg gcgtggtcag cgggtgatcc gctttgaacg ggccttccgg
    14101 catgttgttg aggcactgct caagaatgcg cagactctgg cgcagctctt ccactttcag
    14161 cattacgcgg gtgtagcagt cagaaacacc accaccaacc gggatttcaa agtcgaagtt
    14221 ttcatagcca gaataaggac gcgccttacg cacgtcgaag tcgatcccgg tagcacgcag
    14281 gcccgcgcca gtggtgcccc actccagcgc ctctttcgcg ccataggcgg caacgccctg
    14341 ggaacgacct ttcagaatgg tattttgcag cgccgctttc tcgtaagacg ccagacgttt
    14401 cggcatccag tcgaggaact cacgcagcag acgatcccag ccgcgcggca ggtcgtgcgc
    14461 tacgccgcca atacggaacc acgccgggtg catacggaaa cccgtgatcg cttccaccag
    14521 atcgtaaatt ttctgacgat cggtaaaggc gaagaacacg ggcgtcattg cgccgacgtc
    14581 ctgaataaag gtcgagatgt acagcaggtg gctgttgata cggaacagtt cggagagcat
    14641 aacgcgaatg acgttaacgc gatccggcac ggtgatcccg gccagtttct ctaccgccag
    14701 cacgtaaggc atttcgttaa cgcagccgcc gaggtattca atacggtcgg tatacggaat
    14761 gtagctgtgc caggactggc gctcgcccat tttctccgca ccacggtggt ggtagccgat
    14821 gtctggtacg cagtcgacaa tctcttcgcc gtcgagttgt aaaacgatac ggaaagcccc
    14881 gtgcgccgac gggtggttcg gaccgaggtt gaggaacatg aagtcctcat tttcggtgcc
    14941 gcgcttcatc ccccactctt ccggtttgaa ggtcagggct tccatctcca gatcctgttt
    15001 ggctttggtc agctcaaacg gcgagaattc ggtagcgcgt gccgggtaat ctttacgcag
    15061 cgggtgacct ttccaggttt gcggcatcat gatgcggcgc aggttcgggt gaccgtcgaa
    15121 agtaatgcca aacagatccc aggtttcacg ctcataccag ttagcgttcg ggaacagttt
    15181 ggtgaaggtc ggtacgtgca ggtcgttttc tgccagcgcc accttcagca tgatgtcgcg
    15241 gttacgatcg atagaaatca gatggtagaa aacggaaaaa tccgcggcag gtaacccttc
    15301 gcggtgtgtg cgcagacgtt cgtccatgcc gtgtaagtca aacagcatga cgtaaggttt
    15361 cggcagtttc tttaagaaat cgccaacttc cagtaattgt tcacgcttga tccacacaac
    15421 gggaaccccg gtgcgagtcg cctgaacagt aaaggcatcc ggcccaaaac ggttgcgcag
    15481 ttcgccaatt accggatcat caagatgatc gcgggtctgc caggcgggtt cttgcgcggt
    15541 taagtcggtc atattgttca ccattgcaaa tggtccgtgg tgactgtcgg cgcagtaagc
    15601 ttcgcgaaat agatttagga ataagcgaag aaaaatccct ttgccgacag gcgcaaatta
    15661 aatctcgtca ggtgtacgca ggttggttac ggcaatgcgt tcaccgcgct tgcgttcgcg
    15721 ctctgattgc atattggcgc gataaacgcc ctgatcgcca accacccagg agagcggacg
    15781 acgttctttg ccgatagatt cctgcaacag catcagtgcc tgcatgtacg cttcaggacg
    15841 cggcgggcag cccgggatat acacatcaac cgggatgaat ttatcgacgc cctgcacaac
    15901 ggaataaata tcgtacatac caccagagtt ggcacaggca cccattgaga taacccattt
    15961 tggttccagc atctggtcat acagacgctg aataaccggt gccattttgg taaagcaggt
    16021 tcctgcaacc accatcaggt cagcctgacg cggcgaagca cgcaatactt ctgcgccaaa
    16081 acgcgccacg tcatgcaccg cggtaaacga agtcaccatc tcaacgtaac agcaggaaag
    16141 accgaagtta tacggccaaa ttgagttttt acgaccccag ttaaccatgt cattgagctt
    16201 gcccataaac acgtttttgt taacttcttg ctccagaggg tcggttacga tctcctgctt
    16261 ttgcaggggg taacggtcgt tctcaccgtt gggatctatg cgggtgagcg tataatccat
    16321 cttaatgcct cgcggttagc gttgacgatt agcgatactg ttcgtttccg ggttcatacg
    16381 ctcgcggcgt gaacgcgcgg gcgtccagtc cagcgcgcca atacgcacca gataaaccag
    16441 acctgccagt aacacaaaaa taaaaattgc agcttccaca aagcctaccc agccgctttc
    16501 gcggatagag gttgaccatg cgaacagata cagcgcttca acgtcgaaga taacgaagaa
    16561 catggccacc agataaaact tggcggacag gcgtaagcgg gcggagccga ccgagtcgat
    16621 accggattca aacggcacgt ttttcgacct cgcgcgtgcg cgaccgccta aaaaccaacc
    16681 gcctaccagc atcaggcaac acaggccaat ggcaacgata agaaagatag cgaatgccca
    16741 gtgatgagcg atgacttcag tggatgttga catactcatt gcttactcat caaaagtagc
    16801 gccagattca ctgctcttca cggcagatgg acgccacatc gattcatggg gaggaataaa
    16861 aaaaacctta caataactgt agaaattctt ttatacagct aattgatgtg gtcttttact
    16921 cctttctata accttttgtc aactttaaca aaagcttctt cacattagtt tacataatat
    16981 caacaccatt agcatttaat gccctttcac cccagatcct tgacgactcc aggataatta
    17041 gatgttgttg aatcgtgtcc gttgtgaagc aatggaaaaa atacgggtct attttgacag
    17101 gaatttgtgt cgattcctcc ccccaaaaga gagtattttc ttgatctgtg acacgctttt
    17161 gttattccat aacaaaaacg cagcaacaaa tttacgtatt ttttaacatc attgtagcag
    17221 gtgatttttt tcaggcgatt atttgtgcat tcgggacgtg aatctctggt gattgaaaaa
    17281 taaacagttt tgtacgttct gcactatgcg aggaaggatt tttactaaaa aaaagccgct
    17341 ggggtttaaa acacccccag cggctcgttt tttacactat tgtctcagga attatctatc
    17401 gtccgtcgat tactcgatat ccctttcaat caacaatgaa tcatcccctt ccggagcaga
    17461 cattgggctg tattgccacg gattatggta gctctccatc gcctgataga tcacctgggc
    17521 cagctcatta ttactggacg ggtcatagca cagcaaatat tcggtatcag gtagcggcgg
    17581 taagccatct actccactca gcacacgcag gtccgggctc atcatctcaa ccggccttgc
    17641 cgtcacgcca agaccggctt tcactgccgc acgaacggcc ggaagcgtcg aggcgacata
    17701 agccagtcgc catggaatat ctactttatt aagcgtcgcc agcaccatat cgcgaaacgg
    17761 acttggatca tccagcaata caagagggat cggctcgcct ttttgcaata cgtattccgc
    17821 tgcgcagtac cagtgtgttg gcgaggtacg caggttcaat gccttaaacg ctgaaggacg
    17881 atgggtggtt accatcaaat ccacttcctg ggattccagc atttctgcca tataggcatt
    17941 acgtttaacg cggacatcca gcgctaattt cggataaacc gaactcacgc gatttaacag
    18001 gaaaggtaag atcgtatcgg cagattcatc tgaagcaccg atagttaaca cgccctgaag
    18061 attactgaac attaatgatg agcaggcctc atcattaaaa cgcaggattt tcctggcgta
    18121 accaagaagt tgaatgccat gttcagttaa cagtttgttg cgaccgtgac gagcgaacag
    18181 ttctttccca acgagttgtt ccagacgctg catttgctga cttacggcgg actgagtacg
    18241 acacacagcg gcagctgcgg cagcaaaagt gttcagatcg gcaacagcaa caaatgttct
    18301 cagcagatcg aggtcgaggt taattatcgg acgatttgca cttatcatat attatcactt
    18361 actggcggct catactgagc tggttaatgc tgtgcacaca caaacaagca attccatttg
    18421 taatgtgcct ccctggcagt ttcatccgga acccggacga aagtaaaaat gcatatgagt
    18481 tgcactaaaa aagcgactca cattgttccg ttataatgcc tgaagtagat cacagaatat
    18541 atcttcaggg atcgcatatc tattaagtta ctcactcttt tctatttatg acatgcgcgt
    18601 gtttgtataa atgtaaatgt gagtccttgt tccactctcg tgcagcatcg ctggtcatac
    18661 gcgaacacgt acaaacagca atggtgaagc ggcatcaaga gcagggatcc gtcatttatg
    18721 cgagcatttt accccaaaaa ccttttattt ataagggtca ttgcgaatta tctgatgcaa
    18781 agttatgtta tgttaggcaa agtaatcttc tattattaat aagcacatca aaaccttttt
    18841 gaatattaaa taataattaa ttagcatcat cctcattcat taattccgct taacaatagt
    18901 ttcacacctt acccccattg cgacacctcg gaaatcatca cgctgttaaa ctctccgtaa
    18961 tgcggaaata tcatcatcgc gtttcattag tgaattctta aatgaggcat tttcacacaa
    19021 ttatcttaca gataaaaaaa ccagacttgc aattaagaat tagaacaatt cactatataa
    19081 cattgcatgt aaagcatata agcctcatta tttgtcatta ttaagttatt aacagcacaa
    19141 tcgagccttc ccctctggca aaatcttatt ctgcagacct tcaaaacacc gtcctggggg
    19201 agtacattgt tctaagctga cttccacggc agggagtggc gataacagca aaaaaggtca
    19261 agattcatgt cccccattga aaaatccagc aaattagaga atgtctgtta tgacatccgt
    19321 ggtccggtgc tgaaagaagc aaaacgcctg gaagaagaag gtaacaaggt actgaaactg
    19381 aacatcggca acccagcccc attcggtttt gacgcgccag atgaaatcct cgttgacgtg
    19441 atacgcaacc tgcctaccgc tcaagggtat tgcgattcca aagggcttta ctccgcgcgt
    19501 aaagccatca tgcagcacta ccaggctcgt ggcatgcgtg atgttaccgt ggaagatatt
    19561 tacatcggca atggtgtatc ggagcttatc gttcaggcaa tgcaggcatt gctgaacagc
    19621 ggggacgaaa tgttggttcc tgcaccagat tacccactct ggaccgcggc ggtttcgctt
    19681 tccagcggta aagcggtgca ttatctttgc gatgaatcct ctgactggtt cccggacctc
    19741 gatgatattc gcgctaaaat tacgcctcgt acgcgtggga ttgttattat caacccaaat
    19801 aacccaaccg gcgcggtata ttccaaagag cttttaatgg agattgtgga gattgcacgt
    19861 cagcataatc tcattatctt cgccgatgaa atttatgaca aaattctcta cgacgatgct
    19921 gagcatcact caattgcgcc gctggcacct gacctgctga ccattacctt taacggactg
    19981 tcgaaaacgt accgcgttgc aggcttccgt caggggtgga tggtgttgaa cgggccgaaa
    20041 aaacacgcca aaggctacat cgaaggtctg gaaatgctgg cttcaatgcg cctgtgtgct
    20101 aacgttcctg cgcaacacgc cattcagacc gcgctgggtg gttatcagag catcagtgaa
    20161 tttattaccc ctggcggtcg tctttatgag cagcgtaacc gcgcgtggga actgatcaac
    20221 gatattccgg gcgtttcctg cgtgaaacct cgtggtgcgc tgtatatgtt cccgaaaatc
    20281 gacgccaaac gctttaacat tcatgacgat cagaaaatgg tgctggattt cctgttgcag
    20341 gaaaaagttc tgttggtgca agggacggca ttcaactggc cgtggccgga tcacttccgc
    20401 attgtcacgc taccgcgtgt cgatgatatc gagctgtctt tgagcaagtt cgcgcgtttc
    20461 ctttctggtt atcatcagct gtaatcttaa tttcactgcc ggagattgca tccggcagtg
    20521 ttatcccgcc acaatgacct gatgatgtca tcatacgtaa ggtcactatg aaacagagcc
    20581 atttttttgc ccatctctcc cgcctgaaac tcattaaccg ctggccgcta atgcgcaacg
    20641 tgcggacgga aaatgtgtcc gaacacagtt tgcaggtagc gatggtcgcc catgcgctgg
    20701 cagctatcaa aaatcggaaa tttggcggta atgtcaacgc cgaacgtatc gctttactgg
    20761 cgatgtacca cgatgcctca gaagtgctca ccggcgatct ccctactccg gtgaaatact
    20821 tcaatgcgca aatcgctcag gagtacaagg ctattgaaaa aatcgctcag cagaaactgg
    20881 tcgatatggt tccggaagaa ctgcgggata tctttgcgcc gttaattgac gagcatgcat
    20941 atagcgatga agaaaaatcg ctggtgaaac aggcagatgc actgtgtgca tatctgaaat
    21001 gtctggaaga actcgcggcc ggaaataatg aattcttgct ggcaaaaacg cgactggaag
    21061 cgacgcttga agcgcgtcgc agccaggaga tggactactt catggaagta tttgttccca
    21121 gcttccatct ttcgctcgat gagattagcc aggattcacc gctgtaagca gccggagtct
    21181 gcgtcgcatc agtcaataag cgccggatgc gacatcaggc tcttgtcaaa acggaaacag
    21241 catcgggatc atcaccacac aaaccgccat cacgataatg gtgaacggta cccccaactt
    21301 cacaaagtca ctgaagctgt aatttcccgg acctaaaacc agggtgttaa caggtgaaga
    21361 caccggtgtc ataaaggcgg cggatgctgc catcgccacg accatcgcaa atggataagg
    21421 cgacactccc atcgttttgg cagcagccag cgcaatcgga gccatcaaca ccgccgtcgc
    21481 ggtattagag ataaacagcc caataaccgc cgacaagaca aacaaacacc ccagcatcat
    21541 atgtggccca taaccgccgc caatatccat cagccctttc accgccagcg cgacacctcc
    21601 cgttttctgt aatgccacag caaacggcat catcccaacg atcaaaataa tgctcggcca
    21661 gtgaatggat ttataggcgc tttcagcatc tatacagcgg aatttcccca tcagcaggca
    21721 ggcgatgata gcggcaacag gattaggaat ttcatttgtc agcattaacg ccaccattag
    21781 caccagacag aaaatggcat ggggtgcctg gctgtgcgcg ggcgatgctt cactcacctc
    21841 ttccggtaag ttcagcgcta cgaagtcgcg gccctgtttg gccagcatac cgatcagttt
    21901 ccagttaccc acaaccagga tgatatcgcc cagcagcaga ggctcatccg ccagcgaacc
    21961 ttccagcgcc acgccattgc gcttgagccc caccacattc agtccgtagc gggtacgaaa
    22021 accaatttcg cgcaccgatt taccaatcag ttctgactca ggaattaatg aaatctctgc
    22081 catgcccaca tcaagggcct ggtcagaaaa atactcgccg cgcagtacca tcggctccag
    22141 caattgctca ctacaaaatt gccggagatc gacatcagcc gcagacatat caataagcaa
    22201 aacgtcacgc gcgcgaaatt cagaaacccc attaacgttc acgataacgc gacgaaaacg
    22261 ccgccagcgt tcaacaccga tgacgttagc gccataacgc tcacgtaatt tgagatcatc
    22321 cagccgttga cctatcattg gcgatccggg gcgaatagcc agacgtcgcg cacgcccggt
    22381 cagtcgatat tcacggataa gatcgcgaaa ggttcgacgc gtccagcctt cgcgctgcgg
    22441 ggtctgggta tcccctttca gcatgaaacg catcactaac atatacaaaa tacccagcac
    22501 cagcacgacc aggccaatag gtgttacgct aaagaaactg aagccgtgat agccttcacg
    22561 cagcaattca ctgttgacta ccaggttcgg cggcgtcgcc accagcgtca tcatgccgct
    22621 aatcagcccg gcaaaactta acggcatcat cagacgtgac ggcgaagttt gcatacgcat
    22681 ggcaacgctt aacaccacgg gaataaagat agcgacaacg ctggttgaac tcataaacgc
    22741 gccaagcccc gcgacggtca gcatcaacaa aaccaacatt ttgatttcac tgttgcccgc
    22801 cactttgacc agccatgttc ccattacggt ggcaacaccg gtacggacca aaccatcgcc
    22861 aataataaac aaggcggcaa tcaggacaac gttaggatca gaaaagccgg aaaatacttc
    22921 ggggaccgtc agcgttccgc ttaatgcaaa cgcgacaata acaaacaaag cgaccgcatc
    22981 catacgcact ctgcccgtcg caaacaagac gatggcaacg gccagtaatg aaagaaccca
    23041 aatcaattca ccgttcacaa cttatccttg ttaattgagg gggatgactt gattctgcca
    23101 taaaaaagcc ccgacgagac ggggctaaat catgatcagg tgtttcactg aataataaca
    23161 tcgccatttg gctgtttggt cacagtaatt tgctccagac tgtggaggac caaatcgacc
    23221 tcattcaggc gcggggtatc tgccggagcg ttaaccgcaa tgacatggca acccgccgcc
    23281 aggccagaaa gcacgccagc gggagcatct tccaccacca cacactcctg cggcgcaagc
    23341 cccagcagct gcgcacctaa cagatacgca tcaggttctg gttttccgcg cttcactcgc
    23401 tcagcggtta caaacacctc tggtgcggga agccccgcta ttttatggcg cgctcgcgct
    23461 accggcatgg agccagaagt cacaatggcc cacggaatac ctgctttatt caaatgattg
    23521 agtaaggcga ttgccccagg aagcgcggta ataccttcgg tttccgtggc ctcgatttgc
    23581 tccagacgcg taaactcggc ggcaatatca gcctcggatt tgcccgccat aaaatggcgc
    23641 aaagaggtga tcgcctgttt accgtgaatg aaagccagca cctcttccgg cgctaaccca
    23701 tgacgtctgg cccagttgct ccacgcccgt tctaccgcag gcagggaatc caccagcgtt
    23761 ccatcaagat caaacagaaa acctttgcac cgcacgcggg cctcctcagg cgttgataat
    23821 ttgattaatt tcgttggcgc tcaaatggta ctgacgcggg caggcatgcc acacattaag
    23881 catgcgctga tatttttccc acattggcgt ctgggcgtta aaaccatgag tcccagcgtc
    23941 aaaatgggta tagcgccctt ccacattaac cataaagcgg acataaccga ggtaacgtgc
    24001 ttcagtggcg gcgtcaaagc cgaggaaggt gacacgacgt tcatcgatgg attgctggtc
    24061 ctgcaaatta gaccaagaaa catacaacgc atgatacata tccataatgt cgatgatagt
    24121 gcggcaggtt tcttctttca gctcgccaaa ctcgcgatcc aattcacgca tctgtaatcc
    24181 gtaaccacgc tcaataattg tttgcaggcg acggtaacgt tcagcatttg ccggatcgag
    24241 catagtcatc atcttgtact gattagacaa aataagacgt tgcgcgttgg tcatttccat
    24301 tgttgactcc tgtatcactc tactacggtg aaaaaaaaga aggctgagta tgccttcttt
    24361 tatatgcgta atcaggggtc aattacaaat catcaaggaa agttttatcc agttgtttga
    24421 aggcgcgctt aagcgtgtca gctaatgcct ggtaatcagg cttgccttca acgggtgcca
    24481 acacctgtcc agactcctgc aatttaccgc gaacttcata aaaccagtta aggattgcag
    24541 ggggtaatgg cgttacagaa cgcttgccca gccaccacaa tccctgcatg ggtaaactta
    24601 aggcgaacag tgcagtggca actgccggcc caagcagacc gcccagggca atctgccagc
    24661 agagagtaaa tacggcgatc ggcggcataa aacggatcgc ataacgcgtc atcttgataa
    24721 cgcgattttc gacaaagacc ggagcaaggc gtttttccag tggccacgtc tttgagtaat
    24781 gctgtccgcg gcgaaacaag ctaaaaaaat taacagaacg attatccggc gttgacatgc
    24841 ttcacctcaa cttcacatat aaagattcaa aaatttgtgc aaattcacaa ctcagcggga
    24901 caacgttcaa aacattttgt cttccatacc cactatcagg tatcctttag cagcctgaag
    24961 gcctaagtag tacatattca ttgagtcgtc aaattcatat acattatgcc attggctgaa
    25021 aattacgcaa aatggcatag actcaagata tttcttccat catgcaaaaa aaatttgcag
    25081 tgcatgatgt taatcataaa tgtcggtgtc atcatgcgct acgctctatg gctccctgac
    25141 gtttttttag ccacgtatca attataggta cttccatgtc gagtaagtta gtactggttc
    25201 tgaactgcgg tagttcttca ctgaaatttg ccatcatcga tgcagtaaat ggtgaagagt
    25261 acctttctgg tttagccgaa tgtttccacc tgcccgaagc acgtatcaaa tggaaaatgg
    25321 acggcaataa acaggaagcg gctttaggtg caggcgccgc tcacagcgaa gcgctcaact
    25381 ttatcgttaa tactattctg gcacaaaaac cagaactgtc tgcgcagctg actgctatcg
    25441 gtcaccgtat cgtacacggc ggcgaaaagt ataccagctc cgtagtgatc gatgagtctg
    25501 ttattcaggg tatcaaagat gcagcttctt ttgcaccgct gcacaacccg gctcacctga
    25561 tcggtatcga agaagctctg aaatctttcc cacagctgaa agacaaaaac gttgctgtat
    25621 ttgacaccgc gttccaccag actatgccgg aagagtctta cctctacgcc ctgccgtaca
    25681 acctgtacaa agagcacggc atccgtcgtt acggcgcgca cggcaccagc cacttctatg
    25741 taacccagga agcggcaaaa atgctgaaca aaccggtaga agaactgaac atcatcacct
    25801 gccacctggg caacggtggt tccgtttctg ctatccgcaa cggtaaatgc gttgacacct
    25861 ctatgggtct gaccccgctg gaaggtctgg tcatgggtac ccgttctggt gatatcgatc
    25921 cggcgatcat cttccacctg cacgacaccc tgggcatgag cgttgacgca atcaacaaac
    25981 tgctgaccaa agagtctggc ctgctgggtc tgaccgaagt gaccagcgac tgccgctatg
    26041 ttgaagacaa ctacgcgacg aaagaagacg cgaagcgcgc aatggacgtt tactgccacc
    26101 gcctggcgaa atacatcggt gcctacactg cgctgatgga tggtcgtctg gacgctgttg
    26161 cattcactgg tggtatcggt gaaaatgccg cgatggttcg tgaactgtct ctgggcaaac
    26221 tgggcgtgct gggctttgaa gttgatcatg aacgcaacct ggctgcacgt ttcggcaaat
    26281 ctggtttcat caacaaagaa ggtacccgtc ctgcggtggt tatcccaacc aacgaagaac
    26341 tggttatcgc gcaagacgcg agccgcctga ctgcctgatt tcacaccgcc agctcagctg
    26401 gcggtgctgt tttgtaaccc gccaaatcgg cggtaacgaa agaggataaa ccgtgtcccg
    26461 tattattatg ctgatcccta ccggaaccag cgtcggtctg accagcgtca gccttggcgt
    26521 gatccgtgca atggaacgca aaggcgttcg tctgagcgtt ttcaaaccta tcgctcagcc
    26581 gcgtaccggt ggcgatgcgc ccgatcagac tacgactatc gtgcgtgcga actcttccac
    26641 cacgacggcc gctgaaccgc tgaaaatgag ctacgttgaa ggtctgcttt ccagcaatca
    26701 gaaagatgtg ctgatggaag agatcgtcgc aaactaccac gctaacacca aagacgctga
    26761 agtcgttctg gttgaaggtc tggtcccgac acgtaagcac cagtttgccc agtctctgaa
    26821 ctacgaaatc gctaaaacgc tgaatgcgga aatcgtcttc gttatgtctc agggcactga
    26881 caccccggaa cagctgaaag agcgtatcga actgacccgc aacagcttcg gtggtgccaa
    26941 aaacaccaac atcaccggcg ttatcgttaa caaactgaac gcaccggttg atgaacaggg
    27001 tcgtactcgc ccggatctgt ccgagatttt cgacgactct tccaaagcta aagtaaacaa
    27061 tgttgatccg gcgaagctgc aagaatctag cccgctgccg gttctcggcg ctgtgccgtg
    27121 gagctttgac ctgatcgcga ctcgtgcgat cgatatggct cgccacctga atgcgaccat
    27181 catcaacgaa ggcgacatca atactcgccg cgttaaatcc gtcactttct gcgcacgcag
    27241 cattccgcac atgctggagc acttccgtgc cggttctctg ctggtgactt ccgcagaccg
    27301 tcctgacgtg ctggtggccg cttgcctggc agccatgaac ggcgtagaaa tcggtgccct
    27361 gctgctgact ggcggctacg aaatggacgc gcgcatttct aaactgtgcg aacgtgcttt
    27421 cgctaccggc ctgccggtat ttatggtgaa caccaacacc tggcagacct ctctgagcct
    27481 gcagagcttc aacctggaag ttccggttga cgatcacgaa cgtatcgaga aagttcagga
    27541 atacgttgct aactacatca acgctgactg gatcgaatct ctgactgcca cttctgagcg
    27601 cagccgtcgt ctgtctccgc cagcgttccg ttatcagctg actgaactgg cgcgcaaagc
    27661 gggcaaacgt atcgttctgc cggaaggtga cgaaccgcgt accgttaaag cagccgctat
    27721 ctgtgctgaa cgtggtatcg caacttgcgt actgctgggt aatccggcag agatcaaccg
    27781 tgttgcagcg tctcagggtg tagaactagg tgcaggcatt gaaatcgttg atccagaagt
    27841 ggttcgcgaa agctatgttg gtcgtctggt cgagctgcgt aagaacaaag gcatgaccga
    27901 aaccgttgcc cgcgaacagc tggaagacaa cgtggtgctc ggtacgctga tgctggaaca
    27961 ggatgaagtt gatggtctgg tttccggtgc tgttcacact accgcaaaca ccatccgtcc
    28021 gccgctgcag ctgatcaaaa ctgcaccggg cagctccctg gtatcttccg tgttcttcat
    28081 gctgctgccg gaacaggttt acgtttacgg tgactgtgcg atcaacccgg atccgaccgc
    28141 tgaacagctg gcagaaatcg cgattcagtc cgctgattcc gctgcggcct tcggtatcga
    28201 accgcgcgtt gctatgctct cctactccac cggtacttct ggtgcaggta gcgacgtaga
    28261 aaaagttcgc gaagcaactc gtctggcgca ggaaaaacgt cctgacctga tgatcgacgg
    28321 tccgctgcag tacgacgctg cggtaatggc tgacgttgcg aaatccaaag cgccgaactc
    28381 tccggttgca ggtcgcgcta ccgtgttcat cttcccggat ctgaacaccg gtaacaccac
    28441 ctacaaagcg gtacagcgtt ctgccgacct gatctccatc gggccgatgc tgcagggtat
    28501 gcgcaaaccg gttaacgacc tgtcccgtgg cgcactggtt gacgatatcg tctacaccat
    28561 cgcgctgact gcgattcagt ctgcacagca gcagtaatct cgtcatcatc cacagctttg
    28621 cgctgcggat atctgaaccg gaaataatca ctatttccgg ttttttattc tcttaatttg
    28681 cattaatcct ttctgattat cttgcttaac tgcgctgcat caatgaattg cgccatctca
    28741 ctttgcatac ttaccacttt gttttgtgca agggaatatt tgcgctatgt ccgcaatcac
    28801 tgaatccaaa ccaacaagaa gatgggcaat gcccgatacg ttggtgatta tcttttttgt
    28861 tgctatttta accagtcttg ccacctgggt agttccggtg gggatgtttg acagtcagga
    28921 agtgcagtat caggttgatg gtcaaacaaa aacacgcaaa gtcgtagatc cacactcatt
    28981 tcgcattctg actaacgaag caggcgaacc tgagtatcac cgcgtacagc tgttcacgac
    29041 gggcgatgaa cgcccgggcc tgatgaactt cccgtttgaa gggttaacct caggatcgaa
    29101 atacgggaca gccgttggca tcatcatgtt tatgctggtg attggcggcg cgtttggcat
    29161 tgtgatgcgt acaggaacca ttgataacgg tatcctggcg cttattcgcc atacccgcgg
    29221 gaatgaaatt ctctttattc ctgcgctgtt tattctgttt tcacttggcg gcgcggtatt
    29281 tggtatggga gaagaggccg tcgcctttgc cattatcatc gcaccgctaa tggtccggct
    29341 gggctatgac agtattacca ccgtcctggt gacttatatt gccacgcaaa tcggttttgc
    29401 cagttcgtgg atgaacccgt tttgtgtggt tgttgctcag gggattgccg gcgttccggt
    29461 gctttctggc tccgggttgc gcatcgtggt gtgggttatc gccactctga ttggcctgat
    29521 ctttaccatg gtgtacgcct cacgagtgaa aaagaatcct cttctgtcac gcgtgcatga
    29581 gtccgaccgc ttctttcgtg aaaagcaggc ggatgttgaa caacgtccgt ttacctttgg
    29641 tgactggctg gtattgattg tcctgacagc cgtaatggtc tgggtgattt ggggcgtgat
    29701 cgttaatgcc tggtttattc cagaaattgc cagccagttc ttcaccatgg gtctggtgat
    29761 tggcatcatc ggcgttgttt tccgccttaa cggcatgacg gttaatacca tggcttcatc
    29821 ctttaccgaa ggggcgcgaa tgatgatcgc ccctgccctg ctggtgggtt tcgccaaagg
    29881 gattttgctg ctggttggta atggtgaagc aggtaatgcc agcgtgttaa atactatcct
    29941 caacagcatt gccaatgcca ttagcggtct ggacaacgcg gtcgcggcct ggtttatgtt
    30001 gctcttccag gctgtattta atttcttcgt gacgtccggt tctggtcagg cggcattaac
    30061 tatgccgtta ctggcaccgc ttggcgatct ggtcggtgtt aaccgtcagg ttaccgtgct
    30121 ggcgttccag tttggtgatg gcttcagcca catcatttac ccaacctcag cttcgttaat
    30181 ggcaacgctc ggtgtgtgcc gggtggactt ccgtaactgg ctgaaggtgg gcgcgaccct
    30241 gcttggactg ctgtttatta tgtccagcgt cgtagtgatc ggcgctcagt tgatgggcta
    30301 ccactaaaaa tgttaagagc cgcaattgcg gctctttttt cattctgccg tttcagtctc
    30361 tacggcttca tttttggcat tgcgcttcat ccacaacgcc agcgctttca gcgagtctgg
    30421 agtgaactca tcgcagcgtg cggtgatttc ttccggcgtc agccagcaaa ctccactgac
    30481 ttcatcttcc tgtagggcga agggaccgtg agagacgcaa ctgaacaacg cgccccagac
    30541 acggcaattt ttatcttcga aatagaactg cccgtgctcg gcaaagggga caccggcaat
    30601 gcccaactct tcttccgctt cgcgacgcgc ggattccagc agttgctcat cggcctgaac
    30661 tacaccgcct gcggtcgcat ctaacatgcc gggtaaaaag tcttttgtct cggtacgacg
    30721 ctggaccaga attttgccca tgccatcatg cacgacgatg taagttgcac gatgacgcag
    30781 acactgtgcc cgcatttgtt cccggctggc ttgtgcaatg acttcgttct cttcattgac
    30841 aatatccacc cattcagtac ttgccaaacg acgctgttcc attatcagga aaccttcttt
    30901 ttctggcgct cttacggcgc attttgagtt gtgggtaaat tacggattaa tcgcgacctg
    30961 cgcaatgata ctttgatcat tgagtgcgat tacactaagt acgtcattat ccagcatgcc
    31021 ataacccgcc ggattaccgc ctttcggaat actcaccgag ccggggttga agtggaaaat
    31081 ctccccccgc tgttctgcca ctggcagatg ggtatgaccg tacaccagca catcgttctg
    31141 gtttaaagca ggtagatttt ccgggccaaa aagatgacca tgcgtcaaaa acagacgttg
    31201 tttttccagt aatacctgtt gccacggcgc ggttatcggg aaatgcagca tcatttgatc
    31261 cacttcgctg tcgcagttgc cacgcacagc gataacctta tgtgccactt cattaagccg
    31321 ttcagcgact ttggctggcg cgtaaccctc cggtaaagca ttacgcgggc cgtgattcaa
    31381 cacgtcgcca aggatcacca gccactgggc accgctttgg acaaacaact ccacaacacg
    31441 ttccgtcgcc ggtaacgacc catgaatgtc cgatgcaaac atcagtttca tcactcactc
    31501 ctcgtcgaaa aaacgtaagc tatgatactg gattctgtcg ttgctatcag ccagattgct
    31561 tcgccgaaag tgcaataaaa cgctggacag acgctcgctg ccactggaat gtcgcataat
    31621 ccaccaggcg ttctggcacc tcatcgccat gtagtaccag gcggttaatc attagcgcca
    31681 gatcagtatc agcaatgcac cattcaccaa ataaattcgg ctgacccagt gctaacaaat
    31741 gttctgccat cgcgaacagt ttctctgcac tggcttttcc ctcggccgtt agtggcgctt
    31801 ttttcgcccc cgcaaagaca acatccgtcg gacgctcttc gcggatgggc atcagatcgc
    31861 tgcgcagcca ggcctgaatc tgtcgtgcac gcgcacgatt ttctaaatca agcggataaa
    31921 tacgttccca ggtcggtggc gcaaatcgat cttccagata ctccgcaatg gcagaagatt
    31981 cactcaactc aaaatcatcg atttgtaata acggcacacg gcgtgtctga ccgtaacctt
    32041 gccacgtcgg ctgcaaatgt tcaccgctgt cgaggtcgat ggtcttgata tgaaacgaca
    32101 ggcctttttc ctgcaacgcc acccaggcgg ataacacata aggggagaaa aagtgggcat
    32161 ctgaccaaag cgtgatagcg ggtttactca ttatgtcctc ggcaataaag ggggctggct
    32221 atcaaactat agagcctttt tgtcactgtc acgtgacgaa tactcacgta agtcgccgcg
    32281 ctttctatac ttgtggtgtt cagatgatta aacaaccgga gctgcaatga tcgatctcta
    32341 tttcgccccg acacccaatg gtcacaaaat tacgctgttt ctcgaagaag cagagcttga
    32401 ttatcgcttg attaaggtag acttggggaa aggcggtcag tttcgtccgg aatttttgcg
    32461 catttcgccc aacaacaaaa ttccggcaat tgttgatcat tctcctgccg atggcggcga
    32521 accgttaagc ctctttgagg ctgctgccat tttgttgtat ctggctgaga aaacaggact
    32581 ctttttgagt catgaaacgc gtgagcgcgc cgccacatta cagtggttat tctggcaggt
    32641 aggcggactg gggccgatgc ttgggcaaaa tcatcatttt aatcacgcag ccccccaaac
    32701 cattccttac gctattgaac gttatcaggt tgaaactcag cgtctttacc atgtactaaa
    32761 caagcggctg gaaaactcgc cctggctggg aggcgagaac tacagcattg cggatattgc
    32821 ctgctggccg tgggttaatg cctggactcg cctgcgaatt gacctcgcaa tgtatccggc
    32881 agtcaagaac tggcatgagc ggatccgttc gcgccctgcc accgggcagg cactgctaaa
    32941 agcacaactc ggtgatgagc gttcggatag ttaacagaaa caggttctcg tgtattattt
    33001 catcctaagt aaaacaacgg agaacctgca atggcacaac ctgccgctat tattcgtata
    33061 aagaaccttc gtttgcgtac gtttatcgga attaaggaag aagaaattaa caaccgtcag
    33121 gatattgtta tcaatgtgac gatccactac cccgccgata aagcgcgcac tagcgaagat
    33181 atcaacgatg cgctgaatta tcgcaccgta acgaaaaaca ttattcagca tgtagagaat
    33241 aaccgtttct ctttgctgga aaaattaact caggatgtgc tcgatatcgc acgtgaacat
    33301 cactgggtga cgtatgctga agtggagatc gataaactgc acgcgctgcg ctacgccgat
    33361 tcggtatcca tgaccttaag ctggcagcgt taatcgccat atcgggaggc tgcatgaaca
    33421 tagtgatcac cggagggacg ggattaattg gtcgccattt gattccgcgt ttgctggagc
    33481 tgggccatca aattacggta gtgacgcgta acccgcagaa agccagttcc gttctcggcc
    33541 ctcgggtgac actatggcaa gggcttgccg atcaaagcaa cctcaacggc gttgatgcgg
    33601 taatcaacct ggccggagaa ccgattgctg ataaacgctg gactcacaag caaaaagagc
    33661 gtctctgcca aagccgctgg aatatcacgc aaaaactggt cgatttgatt aatgctagcg
    33721 acacgccgcc gtcggtactc atttccggct cggcaacggg ctattatggc gacttaggtg
    33781 aagtggtggt taccgaagag gaaccgccgc ataacgaatt tactcataaa ctctgcgccc
    33841 gctgggaaga gattgcctgc cgggcgcaaa gtgacaaaac gcgagtgtgc ctgctgcgaa
    33901 ccggcgtggt actggcaccg gacggcggta ttctcggtaa aatgctgcca ccgtttcgtc
    33961 ttggcctggg cgggccgatt ggttccggtc ggcagtatct ggcctggatt catattgatg
    34021 atatggtcaa cggcattctc tggctgctgg ataacgagct gcgcgggcca tttaatatgg
    34081 tttcgcccta cccggtacgc aatgaacaat ttgcccatgc gctcggtcat gcgctgcatc
    34141 gcccggctat tttgcgcgtc cccacaaccg ccattcggct gttaatgggc gaatcttcag
    34201 tactggtatt aggtggacaa cgcgcgctgc ctaaacggct ggaagaagcg ggttttgcgt
    34261 ttcgctggta cgatttagaa gaggcgctgg cggatgtcgt tcgctgatgt ggtttacagc
    34321 aaacatccgc cagttaactc cctgtgttac aggattagtg gctttgcgcg ataagatcgt
    34381 ctggtgaaag tcgggtcacc atcataacta actctctgtc taaacctcta tccagcatct
    34441 cctgagcaat acgcagggct tcttcgtgtt tgccctgcat tgcgccttct tcacgtaatc
    34501 tgtcagcaat ggtcatcagt ttctcctttt cttgtggtgc gcgttctgtt atctcaccaa
    34561 taaatgcacg gaaacgctgg gcatcccctg tttgtaatac gtaattaaac agggctttta
    34621 gctgtctgtc attagtgttc cctgtaacta gcagcgaaac aatttggtcg actaatccca
    34681 acagatcgcg ctgacgaata tgtttctgaa ttaactccaa cagcgccatt ttgcggtgtt
    34741 gcataatctc gtcatccggc accacggtaa tatccaccaa cggaaacgct gatgaatata
    34801 ttttgcgggc tatagcaggc tcggcaaatt catcaagcca gcagagtgaa taaggataag
    34861 gacttctgca accatgataa aacagcatcg ggatcaccaa tggaagctct ttatagcccg
    34921 catcaagatg gttttgcatt gccgcaatgg aataacgcat catgcgaaaa gccattaatt
    34981 cttccggctt actttggtgc tctatcacta cataaatata acccactccc tcctgcgttt
    35041 tcacagacca caagaggtcg gaataatatt gccgcaggtc ttcatcaata aaactgtttg
    35101 gttctagttt aagcgtcgtt aaatcacaca gtttgcgcag cggcgcggga agatgaatat
    35161 taataaaatt ccgcgcggtg tctggatggc gtaaaaaaga tttaaatacc gcatcatgcg
    35221 gcgtggaagt tgtcgatatt gtcatggcgt tccgtcaccc ctcaaattta agatgacgcg
    35281 acaataacca cgattaacac ccaatgcagc aggcagtttt tatctttgga cagcgaattc
    35341 agaagtattt ttagcggact gcaactttta catttcagcg gaagctgact cgcaaattcc
    35401 ccttgaaaat actccggcag cgttagccac cggagaggaa attatttcag cgatccctta
    35461 aggaactgtt gcagacgagg gctttgcggg ttgccaaata actgctccgg cgcgccctct
    35521 tcttctattt tcccctgatg gaggaaaatg acgtgagtag aaacatggcg agcaaagccc
    35581 atttcgtgag tcactaccac catggttttc ccctcttctg ccagttgctg cataatacgt
    35641 aacacttcgc ctaccagttc aggatcgagc gccgaggtag gttcatcaaa cagtaaaact
    35701 tctggttcca tcgccagcgc ccgcgcgata gaaacacgct gttgctgacc gccggaaaga
    35761 tgcaccggat atttcccctg cgcacgttcg tctatcccga ctttcgccag atacttcacc
    35821 gcccgctcgc gcgcttcctg cttgctcagg cccaacacct gaatcggcgc ttccatgacg
    35881 ttttccaaca ccgtcatatg gctccagaga ttgaagtgct ggaataccat cgtcaggcgt
    35941 gtgcgcagta agcgcagttg atttttatcg gcgactttga gttgaccgtc tttgtcgcgc
    36001 accagattga tcgtctggcc attgaccacg atcgatcctt cactcggttt ttcgaggaag
    36061 ttaatgcagc gcagaaaggt acttttcccc gatcccgacg atccgatgat gcttattaca
    36121 tctccggcat tcgcttgcag tgagacccct ttcagcactt cgtgttcgcc atagcgtttg
    36181 tgcaaatcga taacgtttaa tttattctcg gacatcgtat tctcagtgcg ttgaagaagg
    36241 tttcacatgc tgcaaccagc gtttttccgc tctgcgaaag aggctgatca ggacataaga
    36301 gatgattaaa tagagcaccg cggcaatgcc gaaggcggta aaaggttgat acgtggcggc
    36361 gttaatatcg cgggctattt tcagcagatc cggcacagtg gccgtaaacg ccagcgcagt
    36421 agagtgcagc atcaggatca cttcgttgct gtatgccggt aacgcaatac gcagcgcaga
    36481 aggcaaaata atgcagcgat acattttaaa ggtcgagaag ccataggctc tggcggcttc
    36541 aatttcccca tgcggaaccg aacggattgc cccagcaaaa atctcggtag tgtaagcgca
    36601 ggtgttaagc gtcagcgcca gcacggtaca gttcaggcca ctgcggaaga aagcgttaag
    36661 gaattcggtt cccttaacaa tctcaagcgt gtacatgccg gaatagaaca ccagcaactg
    36721 aacatacagc ggcgtaccgc gaaaaatata ggtaaataac cagattggaa actggatgta
    36781 tttattactg gagacacgac caatcgccag aaacagcgcc aggactccgc ctatcactac
    36841 cgacaaaata agcagccaca gagtgatcgc cacaccagta aagcgataac cgtcggtcca
    36901 cagcagcggt ttccagtatt catgtaagat ttcgatcaca ggtcagccct cttcacaccc
    36961 acggagtagc ggcgctcaag gaacagcagc acaccattgg aaacggtggt gaaaaccagg
    37021 taaatcacgc cacagacgat ggcgaaatag aacggttccc aggtactttt gcctgccagc
    37081 tgcgtggctt tgaccacatc ttccaggccg agtaacgaaa ccagtgcggt agatttgagg
    37141 atcacctgcc agttgttgcc aatgcctggc agggcgtaac gcatcatcgc cggaaacatg
    37201 atccgccgaa acacttgccc gcgagtaaaa ccgaacgccg tcgccgcctc tatatgccct
    37261 ttcggcactg ccataaacgc gccacggaac gtttcggtaa aataagcacc gtagataaaa
    37321 ccgagagtga taataccagc gaccatcgga tcgatatcaa tctgcccgac gcccatcgcc
    37381 tccgtcacca tgtttagcgc aatctgcaaa ccatagaaaa tcagcagcat caacaccaag
    37441 tccggcacgc ctcgaatcag cgtggtgtac ccttcgaaaa tcagccccga aagccgattt
    37501 tgcgagagct taccgccagc gccaattaaa ccgatgatta cagcgagcac tacagagctg
    37561 atagccagct ccagcgtgac gagcgcaccc tgtaaaataa cacctgaaaa cccatacaac
    37621 atgctgcctg tcctgtcgtg tgtggtgaat taccgcctct tgtctccctc cggttacccg
    37681 aagggagcgg gctttctttc accgatggcg attaaccacc ataaacatca aaatcgaagt
    37741 actttttcgc taatttctcg taagtaccgt cagcgcgcat ttcggcaaag gctttgttca
    37801 gtgcctcgcg cagttcgtta tcttctttac gcaggcccat gccggtccct acgccaaaca
    37861 gtttttcatc tttaacagac ggaccaccga atttgtaatc tttaccgacg ggttgtttga
    37921 ggaaaccttc gctggcagcg acctcatcct ggaacgcggc atcaatacgt ccggcagtca
    37981 ggtcagaata aatgttgtcc tgcccctgat acgagacgat ttcaatgcct tttggtgccc
    38041 agtgttcatt accgaacgtc tcctgggtgg tgccctgcaa tacgcctacc cgtttgcctt
    38101 tcagcgactc gactgtcggc tgaatgtcag aatttttcgc caccaccaga cgagaatcgg
    38161 cagcgtacag tttgtcggtg aaggcaattt cttgctgacg tttttccgta atggaaagcg
    38221 atgacatgat ggcgtcaatc ttcttcgctt ttaaggacgg gattaacgca tccagcgggt
    38281 tttcgacaaa cgtacattgc gtattgatgc gcttgcacaa ctccttcgcc agctcgatat
    38341 cgaaaccaac cagttcgcct tgtgaatttt ttgattcaaa tggcgcatag gtcgggtcgg
    38401 taccgatgcg gatgttttgc ggaattgcag caagtgccgc agttgcgctg gagaaggcca
    38461 gaaccagaga gagcgatagc accagttttt tcataatgtc ctcaactgac agtcttttta
    38521 tagggaatat ttacaggttt gttacactta tcgtgccata aaattgacca tcaaggcaaa
    38581 atatcccgcc ccgttagacg tattattttt cattaaaaat gcttaagtat gcatttcaat
    38641 gcaccacgat agtgcaacaa acctggcgtg caccatgatg atcattccat caggtacagc
    38701 ttcccagcga cgtatcagtc accgtagaca ttaaagtcga aatacttttt cgccatcttg
    38761 tcgtaggtgc cgtcctgacg cagctcgcca agcgccttat tgaaggcagc cgtcagttca
    38821 gcataatctt tacgtagccc tacaccggtg ccatcaccga agtatttttt gtcttttact
    38881 gatgagccag caaaagcgaa atctttacca gcaggttgct tgaggaatcc ttcgctggca
    38941 gcaacttcat cttgtaacgc agcatccaga cgtcctgcag ccagatcgga atagaccaaa
    39001 tcctggttgg cataggccac cacatccacg cctttactac gccaggtctc gttagcgtaa
    39061 gcttcctggg ttgatccctg cagcacacca acatgtttac ctttcagtga atccagcgtt
    39121 ggctgaatcg gtgaaccttt ggcagcaatc aaacgagaat ctgcggcgta cagcttgtcg
    39181 gagaaggcaa tctcctgctg acgtttatcg gtaatggaaa gcgacgaaat aatagcgtcg
    39241 atttttttcg ctttcagtga ggggatcagc gcgtcaaagt cactggcaac ccaggtacat
    39301 ttcacctgca tccgtttgca catctcgtta ccgagatcga tatcaaagcc aacaaaatca
    39361 cctttagcat ctttcgacga gaacagcgca taggtggtat cggttccgat acgtaccgtc
    39421 tccggtagcg ccgcatagct ggaagccgct gcggagagac cgactaataa agacagagcg
    39481 agaatcgact tcttcataca taaccctcaa gtgaaaatgg cttttttatg ttgtgtattg
    39541 tgttgtgtgt ttgcaggctc tttcatgcag gtcttatgcc atcttgacga caacagtaac
    39601 attcaacgtt aaatatgtta ataagacatt gcattattgt cctgaagttg aagatagcag
    39661 gtatagcggt tgaatcgcag cgtttcgatt gaatggcaga aacaaaatgt cgaggatttg
    39721 atcgcggttg caaaattgcc ctgaaacagg gcaacagcgg agttatgcgc cctgccagcg
    39781 ggcaaagaga tcttcaggaa gggttattgc aaactggtca agaacacgat taaccgtctg
    39841 atttatcaca tcatcaaggg attgcgggcg atgataaaac gccggaacgg gaggcataat
    39901 caccgcaccg atttctgccg cctgagtcat taaacgcaga tggcctaagt gcaatggtgt
    39961 ttcacgcacg cagagcacca acgggcgacg ctctttcagc accacatctg ccgcacgggt
    40021 cagtaagcca tcagtatagc tatggacaat gccggacagg gttttgattg aacagggtaa
    40081 aatcaccatc cccagcgtct ggaaagaacc ggaagagatg ctggcggaaa tatcgcgcgc
    40141 atcgagcgtg acatcagcta atgcctgcac ttcgcgcagg gaaaaatccg tttcgaggga
    40201 taaggtctgg cgcgccgcct ggctcatcac cagatgcgtt tcgatatctg tgacatcgcg
    40261 cagaacctgt aataagcgca cgccataaat cgcgccgctg gcaccgctga tgcctacaat
    40321 gagtcgtttc ataaatagtt gccctgtcag acttgcaggc agactttgca gtatttcggc
    40381 gggagttgca agtcagggtg ccagaccggc accctcagcg aaggcatcat ccttcgttat
    40441 gcatttcgag attttccact tcgttctgac gttgcactgc tttggcgtca tcattacgta
    40501 acgtatcgag gaaatcgagg tagccctgat caacatcttt ggtgacgtag acgccgttga
    40561 acaccgagca ttcaaactgc tggatatccg gattttcagc gcgaacggcg tcgatcagat
    40621 cgttcagatc ctggaaaatc aacccgtcag caccgatgat ctggcgaatt tcatcaactt
    40681 cgcgaccgtg agcgatcagt tccgtggcgc tcggcatatc aataccataa acgttcggga
    40741 agcgaatttc cggtgccgca gaagcgaggt acactttctt cgctccggct tcgcgtgcca
    40801 tctcgataat ctgctcagaa gtggtgccac ggacgatgga gtcgtcgacc agcaggacgt
    40861 ttttatcgcg gaactcggcg cggttggcat tcagtttacg gcgcacggac ttacgacgca
    40921 gctgctggcc cggcatgata aaggtgcggc caacatagcg gtttttaacg aagccctggc
    40981 ggtacggttt gcccagaata cgagcaattt ccagcgcgat atcacacgag gtttctggga
    41041 tcgggatcac cacgtcgata tccagatctt cccattcgcg ggcaattttc tcgcccagtt
    41101 tcgtgcccat attcacacgc gcgctgtaaa cggaaatttt gtcgataaac gagtccgggc
    41161 gggcaaagta tacatactca aacaggcacg gattgctgac cggattgtca gcacattgac
    41221 gggtaaacaa ctgcccttct tcagtgatgt aaatcgcttc gcccggcgcg acgtcacgca
    41281 ggaaatcaaa gcccagcgta tcgagcgcta cgctttcgga agcgaccata tattctgtac
    41341 ggttctcgtc aatatcacgt tttcccagta ccagcggacg aatcccgttt ggatcgcgga
    41401 aagcaaccat accgtggccg ataatcatcg ccacacaggc atacgcgccg cggattaagc
    41461 ggtttgtggc agcaatggca gcgaaaatat tgtcggcttc cagcgggtag tggcggaagt
    41521 tgtccagctc gctggcgaag atattaagca gaatttccga gtcggaagtg gtgttgatgt
    41581 ggcggcgttt ttcttcaaac agttttttac gcaactcgtg agcgttggtc agattgccgt
    41641 tgtgggcaag cgtaatgcca tacggggagt taacgtaaaa cggctgcgct tcagaggcgc
    41701 tggagctgcc agccgtaggg taacgcacat gaccaatgcc catattgccc tgcaaacgct
    41761 gcatatggcg agcttcaaat acatcgctca ccagcccgtt cgctttacgc aaacggaagc
    41821 agttattggc atctatggtg atgatgccgg cggcatcctg accgcgatgc tgaagcaccg
    41881 ttaaggcatc ataaatcgac tggttaaccg gcataacacc ggcgataccg acaataccgc
    41941 acatacgtct ttttcctcgt taagccacat ctcagagcac ttacgctctg ggcaagaaac
    42001 ttgacgagct ttgcagataa tcaaaaaacc atctgatgat aaaactgaat tgcgggatca
    42061 gctgtgattt gctccagtct tcacttttcg acaccccggt aaaggagtcg agaaagaaga
    42121 gaatggcggc aacaatcaac acaccgcgca acgcaccgaa acagacgccc agcacccgat
    42181 cggtgcctga caaccccgtt ttctccacca actggcctat cacgaagttc acgatagcac
    42241 caacgatcag ggtagcgata aacagtaccg cgatggcaat cccatttcga accagttcgt
    42301 cttcaaagcc cgtaaaccag actgacaggt aagtgtagta atgactggca acaaagaaag
    42361 cacaacccca tgtcaccagc gataacgctt cacgaacaaa gccgtggatc aggctaacca
    42421 gagaggaaaa agcaatcacc gcgattatgg cgtaatcaat ccagaccata tgtgtcccac
    42481 gattttacgc cctgtcatcc tgttcggggc gcattctaac agaaaaagaa aacgtttgcg
    42541 tagggatttc cttcccgcgc atcaataaaa atggcgctga aaaaatattc aacgccatcg
    42601 actttttatg cctttgcggc atcgtgcaat gcgtgtcgga tgcggcgtaa acgccttatc
    42661 cgacctacgg ttctaacact gcccaagcct gataagacgc gccagcgtcg catcaggcaa
    42721 gactgtatta attcggcgta tagcccatta ccacgccact taagccagaa agttgcttca
    42781 actcacccag cgaacctttc agcttatctt tcgaggcatc cggcccaacc agaatacggg
    42841 taattttacc ctgcactggc gtggatggcg acgtataaac ccgataaccg gcaccgcgca
    42901 gcttaccgac aatctcattc actttatcgg catttttcag cgcacccagt tgcacaacat
    42961 aggctttacc cgttggcgct gctttttctt ccacgaccgg cttcggttct ggcttcggcg
    43021 caggtggtac ttcaaccttt ggtttaggcg gctccaccgg tttcggcttc ggtggggcga
    43081 ccggtgcagg ttccggttca aactcggtgt tattggctgc aatagtggcc ggatcgagcg
    43141 acggtgctgc ggcatcacct gcccgcacct cttccgctgc gccttccggc ggctgcgtcg
    43201 gcaacgcctg ggtggcggct ggcatcatat caggctcatc acgatcgccc gctttcggca
    43261 ccagcgggat agccgcgaac tcatcctgat aatgtttttt ctgcccgtcc agcagccctg
    43321 gaagtacaat cacccccagc gccaccagca cgatcgtgcc cactaaccga ttctgaaact
    43381 tacttgccac cgcttctcct cgcgtcaatc acttccatga catgtgcgac cgtgtggaaa
    43441 gaaccacaca ccagcacggt gtcttccgct ttagcgtccg ccattgcggc atcccatgcc
    43501 tgcgcaacgc tatcaaatga tttgccgtta cccaaatgct caagcagttg ttctgccgtg
    43561 gcaccgcgcg gcccttccag tggcgcacaa taccagtcat caaccacgct tttcaaccag
    43621 gccagagttc cggcaatatc tttatcatgt agcataccga taaccgccag cacgcgcccg
    43681 tttttcggta gcgctttcat acgcccggtg agatattccg ccgcatgtgg attatgcgcg
    43741 acatcaaaaa taacgcgtgg cgactcgctc acaatctgga aacgtcccgg caaaattgcg
    43801 ctggcaatcc cgtcgcgaat ggcattttca ctgacttcca gcccgctggc acgcagtgcc
    43861 gccagcgccg ttgcggcatt cggttgcggg acaagcggca acggcagatt ttccagcgtg
    43921 ccgtgagcat cgctaaacgc ccagtcatga tcggtgacgg aatagttcca ctcaacgccc
    43981 cgacgttgta acagtgcacc tttttcctgc gccacatcag caatggtaga aggcatttcc
    44041 ggctcaccga caattgccgg tttttcgctg cggaagatgc ctgctttctc gagaccaata
    44101 ctttcgcgat ctggacccag ccagtcggta tgatccagcg caatactggt tactaccgcg
    44161 acatcggcat cgacaatatt ggttgcgtcc agacgaccgc ccagccctac ttccagaatc
    44221 accacgtcaa gttgtgcctg cttgaacagc cacaacgccg acagcgtacc gtactcgaaa
    44281 taggtcaggg aaatatcacc gcgtgccgat tcaatctccg caaaagaggc ggtgtgggcc
    44341 gattccggca attcctggcc ctgcacacgt acgcgctcgg tataacgcac cagatgaggc
    44401 gaactgtaga cgcccacttt gtaccctgcc gccatcagaa tcgactccag cgtacggcag
    44461 gtggtgcctt tgccattcgt acccgcaacg gtaaacacaa atggcgctgg tttcaggacg
    44521 ccaagacgcg ccgcgacctg gctcacgcgc tcaaggccga gatcgatagt tttactgtgc
    44581 aggttttcca gataagaaag ccacgaagcc agaggcgacg cggcttgagg agtgcgtttg
    44641 ataatcatgg tatccgctga ttcgttacgg tgagaatagc aaaagggcag agccagtggc
    44701 cctgccctta tcagttatca ggcctcaggt tcctgatccg gtaccggggg taccactacg
    44761 ccttcacgcg gcgcttcagg attcggcgct ggcagattca tcaacttcgc cagaatgctc
    44821 gccagtttca ggcgcatttc cggacgacgg acgatcatgt cgatcgcgcc tttctcgatc
    44881 aggaattcac tgcgctggaa tctaggcggc agtttttcgc gaacggtctg ttcgataaca
    44941 cgcggaccgg caaagccgat taacgcttta ggttcagcga tgttgagatc gcccagcatg
    45001 gcgaaacttg cagaaacacc gcccatcgtc gggtcggtca gcacggagat gtacggcaag
    45061 ccgcgctcct gcatttttgc cagtgccgca gaggttttcg ccatctgcat cagcgacatc
    45121 agtgcttcct gcatacgtgc gccaccagag gcggagaagc agatcagcgg gcagttatct
    45181 tcctgcgcct gctcaacggc acgcacgaaa cgtgcaccca caacagaccc cattgaaccg
    45241 cccataaagg cgaactcgaa tgccgcagcg acaaccggca ttccatacag agtgcctttc
    45301 atcaccacca gcgcatcttt ttcgccggtt tctttctgcg cagatgccag acggtcttta
    45361 tacttcttgg agtcacgaaa cttcagctca tctttcggct caagctcgct acccagctcc
    45421 acaaggcttc cttcatctaa caggctatgc aggcgattac gcgctgtcat acgcatgtga
    45481 tggtcacact tcggacagac ctcaagatta cgttccagct cagcgcggta taaaacctga
    45541 ccgcagctat cacacttagt ccacacccct tcaggaatgc tcgccttgcg ggtgggagta
    45601 atgttgcttt taattcgttc aatccagctc attagggacc tttctgtctg aacctggttc
    45661 gatgccagtt ttatctttgg ggacgcataa tgccattttt gcccccaaca gaccatgaat
    45721 gttgcacatt aaaacataac agcccgaaac tttggataaa aaagtggtcg aaccgcggag
    45781 ttacttttta ttttgcggcg cgtgccgcag cgcgtttgtg acggattatt tcgatgacgc
    45841 ccggcaaaat agaaaccaca ataatcccga cgatcagcag cttaaggtta tcctgaacca
    45901 tcgggattgt accgaagaaa tagcctgcgt aggtaaaaag cagtacccac aacagtgcgc
    45961 cgatcacgtt ataagcagcg aaatgacggt acgacatgtg gcccattccc gcaacaaacg
    46021 gggcgaacgt tctgacgatc ggcacaaaac gggcgagaat aatcgttttg ccgccatgtt
    46081 tctcataaaa ctgatgggtt ttgtcgagat aactgcgacg gaaaattttc gaattgggat
    46141 tactgaataa cttttcaccg aacaaccgcc cgatagtgta attgaccgcg tcacccacaa
    46201 tcgcggcgat cagcatcagc actaccatca tatggacgtt gagatcgttg gtttccagtg
    46261 atgccaacgc tccggcaaca aacagcaatg aatcacccgg taaaaacggc attaccacca
    46321 gaccggtttc acagaacaaa attaagaaca aaatggcata aacccagacg ccgtactccg
    46381 cgactagctc tgccagatgc acgtcaatgt gcaggataaa atcgatgagg aaataaatca
    46441 ggtccatatt tgcctatgcc ttgtactcgt catctttcag gctgtaacta cgttggctac
    46501 agcctgaaat gttccgagca ttattctcgt tagtccgcca gaaatagcgg gcccattggc
    46561 ggttttggaa gatcataccg gtcagggtaa tccaccgcga ccagatacag cccttccgct
    46621 tttgccgttg ctgccgccag cgttctgtcc tttgccgcca gcaactctgc tatccagctc
    46681 tccggctggt tgtgggcacc gacttccatc aggctgccga caatattcct gaccatatga
    46741 tgtacaaagg cattcgcttt gatatcaacc accacataag gaccgtgacg cgtgacatta
    46801 atatgcataa cgttgcgcca cggggttcgg gactggcact gcaccgcacg gaacgaggtg
    46861 aaatcattct cgcccagcaa gcattgcgca gcccgatgca tccgttcagc gtccagcggt
    46921 tcgtaaaaat gggttacccc tttactcagt accgccgggc gcagccgatg attgtagatg
    46981 atgtagcgat aacggcgagc cgtggcgcta aatcgggcat gaaaatcatc aggtacagct
    47041 ttaacccaac gcacggcgat gtcaccaggt aaattcgcat ttacgcccaa ggtccacgcc
    47101 gcgtctttgc gctgcgcggt tgtttcgaaa tgcaccactt gcccggtacc gtgtacccct
    47161 gcgtcagtac gcccggcgca gaagacggtg atgggttcgt tcgccacctg ggagagcgcc
    47221 ttttccagct tctcctgcac actgcggact tcattctgcc gttgccagcc gtaatactta
    47281 ctgccgtcgt actcaatgcc cagcgcaatt ttataaactg gcagttgttg ctggtcggac
    47341 attagtacag atactcctgc accagtttct cggcgatttt tactgccatc agcgcgccgc
    47401 caaagcgaac gttatcggcc accgaccaga actggacttg ctccggcata ccgtagtcat
    47461 tacgcacgca gccaacagaa agatgcggcg tacccgaagc atcccctacc tgagtcggga
    47521 attcgttctc ttcagagagc acaatatctt cgccctgagc aaacgcatca cgcgcttctt
    47581 ctgccgccag cggacgcagt gcttcaaagt tgaccatctg ggcatgaccg tagaataccg
    47641 gtgcctggac gacgcttgcc gaaatcatca gcccttcgtc ctgcaggatt ttgcgtactt
    47701 cgtcaacgat acgacgttct tcacgcacgc taccttcgct atccggcaac aacggcagca
    47761 tattgaacgc cagttgacga ccgaagaaat cttcttcgtc aatcggaatg ccgttgagca
    47821 atttcgcact ctgccccgct aacgcatcga ccgctttttt gccctgggcg gaggctgaaa
    47881 tcaggctggt aacactgata cgcgacaaac cgccctgatc gattaacggt ttcagtgccg
    47941 ccagcagctg gctggtcaga ctgtctggta cggcgatgac attccggttg cggtaatcgg
    48001 tcagtacaaa cgggtttact tccggcacca ccagcggtac gtcgggttcg agagcaaaca
    48061 gtccgctgct gtcgatcacc aggcaacctg agttggtcgc ttcttcaacc caggcagcgg
    48121 tagcttcttt gcctgcgaca aaaaatgcca gctgcgcctg cgtccagtcg aattcagcgg
    48181 catcctgcac ggtgattgtt ttaccaccaa agcgcagttg ttcgcctgcg ctttcgttac
    48241 gtgccagtgc ataaatttcc ccaaccggga actgacgttc agccagcgtt tcaagcaggg
    48301 cttcgcccat agcgccagtt gcgcccagga cggcaatgtt ccagccttca gacatggtgg
    48361 tttactccag aaatagcaaa gctccctgcc aatgttacag cagagagcat gaagaagaga
    48421 ttaacgtgcc ggatgatgaa cggcgttaaa acccagttta cacagcaatg atgccgcact
    48481 ggcgtcatca caaattacat acagagatga ccattcacgg cgctcaagat agtttttgcg
    48541 cagtttatcg aactcacccg gtatcccggc gactttacgc agcggtgcgt catcgcggcg
    48601 cacatcatac accaaatgca ccagcctttt cagcgtcggt tgatcgagcg gaccatgcag
    48661 cgtaatgcgg ccaaactcag gcgcgggcag caacgtatcc agcgcaacgt gttgttcatg
    48721 cccaataaac ttgctatagg cttcaaacac ttgcgtcgtg ccacgcgctt taccctccag
    48781 ggtatagcct gcgatatgcg gcgtgccgat atccactttt gtcagcaatt ccacattaag
    48841 ttccggctcg ccttcccaga catccagcac cacgcttaac ttctggcctt cattcaggca
    48901 ggtcagcagc gcggtattat cgacgactgc gccacggcag gcgttaatca gaatcgctcc
    48961 gggcttcagg ctacggatca gtttttcatc cgccagatgt agcgttttgt acggaccatc
    49021 tttaaagagt ggcgtatgga aagtcagaat atccgcgcgc tggactaact catccagcga
    49081 gcggaaatca ccctcatccc cacggtcggc gcgaggcgga tcgcaaagta aggttttaat
    49141 ccctaacgct tccagtcgcg cctgcaatcg acgtccaacg ttaccaacgc ccacgatccc
    49201 caccgtacgg tcgtgcagtg aaaatccatc gcgttcggca agcatcagca gggaggagaa
    49261 aacatattcc accaccgcaa tcgcattaca gccaggtgca gcggaaaaac caattcccgc
    49321 ctgcttcaac catgcttcat cgacatggtc ggtccccgca gtggcagttc caacaaattt
    49381 aatgggtttt cctgccagca aagattcatt cactttcgtg accgaacgca ccatcagcgc
    49441 atccgcgtct gccagttgag cgacggggat tggacgcccg ggaaccgcga tcacctcacc
    49501 caaacggcta aataagtcgc gggcataagg catattttca tcaacaagga ttttcacgtt
    49561 tgtgttacct gtatgagacg agagttaacc ggacaagtat gccataatct cgcggccagg
    49621 catacttgcg aagatttcag gtataaggat acgtaatgat acaacctatt tccggccctc
    49681 ctcctgggca gccaccaggt cagggagata acctgccgtc tggcgcgggc aatcagcctt
    49741 tatccagtca gcaacgtact tcgctggaaa gcttaatgac gaaagtgacc tcactgacgc
    49801 aacaacaaag agcagaactg tgggcgggta tcaggcacga tattggtctg tcgggagatt
    49861 caccgctgct ttcgcgtcac ttccctgccg ctgagcataa tctggcgcaa cgtctgctgg
    49921 ccgcgcaaaa aagccattct gcccgccagc ttttagcgca attaggggag tatttacgtc
    49981 tggggaatca tcgtcaggcg gtcacggatt atatccgtca taactttggt cagacgccgc
    50041 tgaatcagct ctcaccggag caattaaaaa ccattctcac cctgttgcag gaagggaaga
    50101 tggttattcc gcaaccacag cagcgcgagg cgaccgaccg tcctttatta ccggcggagc
    50161 acaatgcgct aaaacagctg gtgaccaaac ttacggcggc aacgggggaa cccagcaaac
    50221 agatctggca atcgatgctg gaactttccg gggtgaaaga tggcgagtta attccagcga
    50281 aactgtttaa ccatctggtg acctggctac aggcgcgtca gacgctaagc cagcaaaata
    50341 cgccgacgct ggaatcacta cagatggcgc taaaacaacc tttagatgcc agtgaactgg
    50401 cggcgttatc ggcatatatc cagcaaaaat atggcctttc tgcgcaatca tcgctttctt
    50461 ctgcccaggc cgaggatatt cttaatcagc tttatcaacg gcgggttaaa gggattgatc
    50521 cgcgtgatat gcaaccgctg cttaatcctt ttccaccgat gatggacacg ttgcaaaata
    50581 tggcaacgcg tcccgcgctg tggatactgt tagtcgcgat tatcctgatg ctggtctggc
    50641 tggttcgtta accccgacga aatgacagta tcgtgacaat aatacccagc accgcagaga
    50701 tcgccccggc aagaaatacc gaagagtaac caaacgtggt cgccagcatt cccgccagcg
    50761 gcccggagac gccgagggcg atatcctgaa acgcggcgta accgcccagt gcggtgccgc
    50821 gaacttgtga ggggacgcgt ttaaccacct ccacgcccag cgcaggaaag ataagcgaac
    50881 atccggctcc ggttaacgcc gcgcccgcta atgcgaccca tgcacctggg gcttgccaga
    50941 gcagcaacaa gcccaccgtt tctacaagca gagagacaat cgccactttc acgccgccaa
    51001 aacggtccgg catccagcca aacatgacgc gcatcacgac aaatgcgccg ccaaacgcgg
    51061 taagagtaaa gcccgccatc gcccatcctt tgctggcaaa gtagagcgaa acgaaagtcc
    51121 cgataaccgc aaaaccaacg ccttgtagtg ccagccctaa ccctggtttc cagataagcc
    51181 cgacaacgct ccacagcgat ggacgttctc ccgccagggc cggtactttg cgcactgtgc
    51241 cgttacaggc ccacgccagt aagggtaata ccattgtggt gatcgccagt gcggcaaaac
    51301 cgtaatggct atgaatcaac aggccaagcg gcgcaccaac agcgagggca ccgtaaatcg
    51361 ccattccgtt ccatgacatc actttgccag agtgttttgg ccctacgatg cctaaccccc
    51421 aggtcagagc gcctgtcagt aactggcttt caccaaaccc aagaatcaaa cgcccgatga
    51481 ccaacagggc aaatttgaac ggtgcggaga caggcaaaat cgccgccagc agcaacgcgc
    51541 cgccagccag accacaagct aacatcccct gaagcgccga acgttttgcg ccatattgat
    51601 cggccagtcg cccggcgtaa ccacgcgtca gcaccgtagc cagaaactga atcccgacgg
    51661 caatgccgac catggtattg ccatagccca gatcatgatg aacaaacagc gggataaccg
    51721 gcaacggcaa ccctacggtc atgtaggtga gaaaaaccgc aaaagagatg cggaagagcg
    51781 aaaaactggc agaagatcgt gtttcggttt ggcttacagc agtcatgcat tactccagaa
    51841 tgcagcgcaa ggcgaggagt atccccgtct catctctctg gtttcagggt tacggtgcgt
    51901 tggcaggatt taacgcgtac gtcttttcag aaggaaatcg acaaagcggg aagtttgcct
    51961 agaactggtg gcgattgtca atgatgtgaa aaagggaacc atcaggttcc cttttgcgtt
    52021 agtgccggag gccgcattgg cgcgtaacgt cggatgcgac gctggcgcgt cttatccgac
    52081 ctactccggc ctactgcgaa ttaatctttt cagcttgcgc attaccagcg tggcgttggt
    52141 gccgccgaag ccgaagctgt tagacataac agtggtcagt tcgcgatcgg tcgtttcggt
    52201 cacgatgttc agacccgcag cctgctcgtc cagctcttca atgttgatgc tcggggcgat
    52261 aaagccgtgt tccagcatca gcagagagta gatagcttcc tgcacacccg cagcgcccag
    52321 tgagtgaccg gtcatggctt tggttgcaga aatcgccggg ctcttatcgc cgaacacttc
    52381 acggatagct gccagctctt tcacgtcgcc aaccggagtc gaagtaccgt gagagttcag
    52441 gtaatcgatt ggggtatcaa cgccatgcat cgccatctgc atacaacgta ctgcgccttc
    52501 gccagacgga gcaaccatgt ctgcaccatc agaggttgcg ccgtagccaa cgatttcagc
    52561 atagatgtaa gcaccacgcg ccagcgcgtg ttccagctct tcaaccacta ccataccgcc
    52621 gccgccagcg ataacgaaac cgtcacggtg agcgtcgtaa gtacgggagg ctttttccgg
    52681 ggtgtcgttg tatttagtag atagcgcacc catcgcgtcg aattcgcagg ccatttccca
    52741 gcacagctct tcgccgccgc cagcaaacac gatgtcctgt ttgcccagtt ggatctgctc
    52801 tactgcgtta ccgatacagt gtgcggaagt cgcacacgcg gagctgatgg agtagttaac
    52861 gccatgaatt ttaaacggcg tggcgagaca ggcagaaacg ccggatgcca tcgctttagt
    52921 gaccacatac gggccaaccg ctttcaggcc gcgcgggccg cgcattgcgt cagcgccgaa
    52981 cacctggaaa cgcggggaac cgccgccgga acctgcaatc aggccaacgc gcgggttatt
    53041 ctggtaagct tccggagaga ggcccgcatc agcgattgcc tgctccatag aaaggaatgc
    53101 ataaatggat gcgtcgctca taaagcgcac aactttgcgg tcaatgaggc cagtggtatc
    53161 cagttttatg ttgccccaga cgtggctacg catgccggaa tccttcagct cctgagagaa
    53221 agtgatccct gaacgtcctt cacgcagaga tgccaggact tcctgctggt tattaccgat
    53281 gctggaaaca atgcccaggc cagtaatcac tgcacgtttc attcaatacc tctgtaagtc
    53341 gcacatagag taagtttcga atgcacaata gcgtacactt gtacgccgaa caagtccgat
    53401 cagccattta atagagatat ttgcgcagcc ttacacacat cgctaagatc gagccaccgc
    53461 ctgtaagacg agtaacttac gtgaaacact actccataca acctgccaac ctcgaattta
    53521 atgctgaggg tacacctgtt tcccgagatt ttgacgatgt ctatttttcc aacgataacg
    53581 ggctggaaga gacgcgttat gtttttctgg gaggtaacca attagaggta cgctttcctg
    53641 agcatccaca tcctctgttt gtgatagcag agagcggctt cggcaccgga ttaaacttcc
    53701 tgacgctatg gaaggcattt gatcagtttc gcgaagcgca tccgcaagcg caattacaac
    53761 gcttacattt cattagtttt gagaaatttc ccctcacccg tgcggattta gccttagcgc
    53821 atcaacactg gccggaactg gctccgtggg cagaacaact tcaggcgcag tggccaatgc
    53881 ccttgcccgg ttgccatcgt ttattgctcg atgaaagccg cgtgacgctg gatttatggt
    53941 ttggcgatat taacgaactg accagccaac tggacgattc gctaaatcaa aaagtagatg
    54001 cctggtttct ggacggcttt gcgccagcta aaaacccgga tatgtggacg caaaatctgt
    54061 ttaacgccat ggcaaggttg gcgcgtccag gcggcacgct ggcgacattt acgtctgccg
    54121 gttttgtccg ccgcggtttg caggaagccg gattcacgat gcaaaaacgt aagggctttg
    54181 ggcgcaaacg ggaaatgctt tgtggggtga tggaacagac attaccactc ccctgctcca
    54241 cgccgtggtt taatcgtacg ggcagcagca aacgggaagt agcgattatc ggcggtggta
    54301 ttgccagcgc gttgttgtcg ctggcgttat tacggcgcgg ctggcaggta gcgctttatt
    54361 gcgcggatga agcccccgcg ctgggtgctt ccggcaatcg ccagggggcg ctgtatccgt
    54421 tattaagcaa acacgatgag gcgctaaacc gctttttctc taatgcgttt acttttgctc
    54481 gtcggtttta cgaccaatta cccgttaaat ttgatcatga ctggtgcggc gtcacgcagt
    54541 taggctggga tgagaaaagc cagcataaaa tcgcacagat gttgtcaatg gatttacccg
    54601 cagaactggc tgtagccgtt gaggcaaatg cggttgaaca aattacgggc gttgcgacaa
    54661 attgcagcgg cattacttat ccgcaaggtg gttggctgtg cccagcagaa ctgacccgta
    54721 atgtgctgga actggcgcaa cagcagggtt tgcagattta ttatcaatat cagttacaga
    54781 atttatcccg taaggatgac tgttggttgt tgaattttgc aggagatcag caagcaacac
    54841 acagcgtagt ggtactggcg aacgggcatc aaatcagccg tttcagccaa acgtcgactc
    54901 tcccggtgta ttcggttgcc gggcaggtca gccatattcc gacaacgccg gaactggcag
    54961 agctgaagca ggtgctgtgc tatgacggtt atctcacgcc acaaaatccg gcgaatcaac
    55021 atcattgtat tggtgccagt tatcatcgcg gcagcgaaga tacggcgtac agtgaggacg
    55081 atcagcagca gaatcgccag cggttgattg attgtttccc gcaggcacag tgggcaaaag
    55141 aggttgatgt cagtgataaa gaggcgcgct gcggtgtgcg ttgtgccacc cgcgatcatc
    55201 tgccaatggt aggcaatgtt cccgattatg aggcaacact cgtggaatat gcgtcgttgg
    55261 cggagcaaaa agatgaggcg gtaagcgcgc cggtttttga cgatctcttt atgtttgcgg
    55321 ctttaggttc tcgcggtttg tgttctgccc cgctgtgtgc cgagattctg gcggcgcaga
    55381 tgagcgacga accgattccg atggatgcca gtacgctggc ggcgttaaac ccgaatcggt
    55441 tatgggtgcg gaaattgttg aagggtaaag cggttaaggc ggggtaatct gctctcgcag
    55501 tgtttgccgg atgcggcgtg aacgccttat ccaacctacg tgtgacgtgt aggcctgata
    55561 agacgcgata agcgtcgcat caggcatggt gctccagacg ccgcaactta cagctgtgac
    55621 gcctgctgaa acaggttttc ccacatatcg gtcaccagcg cctggtcacg cggcgacaat
    55681 tcaccggcac caatggcttt ttccagactc tggctaacgg tcgtatgcac cgcctgagcg
    55741 gagtggtcat caccactttc cagttctgcg atggctaatg tcaggtggcc acgcaaatac
    55801 ccactggcaa acaactcatc atcactggca tggtcaacca tgccgtcgat taatgccaga
    55861 atgcgtgatt caaactccgc gatcatcttc tttcctcatt gtaaaacgtg gcgctggctt
    55921 cagttgagcg cttccggcca cgggaactgt tccgccgtaa gttccggcgt gtgataataa
    55981 ttttgtagtg ctttaatgaa gcgtgccggg cgttccggaa tgccatttgt cagataatcc
    56041 atcacctgcg catgtacccg ccgttgaaac accacacgat ccggttcgaa atccccttcc
    56101 agattgtcgc aactgacgtt aaacggatat ccagccgcca cgcagaataa ccagtcgaac
    56161 gcctgcggct tcacttcaac atcttcaaac tggctttgcg tctgggcatc gcgtccgtcc
    56221 gggcaatacc agtagccgaa atcaaccagc tcacggcgcg cttttccggc aatacaccag
    56281 tgcgaaatct catgaattgc gctggcataa aagccatgag caaagacgat tcggttatac
    56341 ggtacttccg catcagcagg aagatagatc ggttcgtcgt cgcctttaat cagacgggta
    56401 ttaaattcat cggcaaagca gctattaaaa atttcaatca actgctcgta gtggtgtgta
    56461 ctgttcatta gttcatcccc aaccagtgga ggatctcctg tccgtggcta tcgtaaagta
    56521 atttggcact catcaccgcc gagacgataa caatcatcgg gcggatcagc ttttgtcctt
    56581 tgctcaacac caaccgtgaa cccatgcgcg cgccgaggaa ctgccccacc agcatgacaa
    56641 agcccgtcgc ccaaatcact ttgccgccga gaataaacag cagcagaccg ccaatgtttg
    56701 acgttgcgtt gagtaatttg gcgtgagccg tggctttggc gaggttgaat ccgcacagca
    56761 taacgaaggc cagcgcgtaa aacgatccgg cagccgggcc aaagaatccg tcataaaaac
    56821 cgacgcagcc accggcaatt aacgcgaagg gtaaaccgta catccggcgc tggcgatctt
    56881 cttcacccag ctttggcatc agcaagaaat agaggccgat acaaatcacc agaatgggca
    56941 aaatctgccg taagacatca gcctgaacgt actgtaccaa cagtgcgccg ctcattgagc
    57001 cgacaaaggt catggcaata ttgagtttct gatcgcttaa actaaccact ttgcggcgaa
    57061 taaagtagat agtggcggaa atagagccgc cgcaggcttg cagtttattg gttgccagcg
    57121 cgttagcggg agacattccc gcagccatca atgccggaat ggtgagtaac ccaccgccac
    57181 cggcaatcga gtcgataaat cccgccagca tggcgacaaa aaagagaact cccagcaaca
    57241 gcggagaaac cataaacagg ctattaaacg tttccattag atcacgtgct catccagtag
    57301 cgcctggcag gaaggcggca acggaggcgg tgtcttcttc tcaggctttg ttgttcccgg
    57361 ttttggaggt tcaaaccagc tttgcagttc tgccccgcaa ccatcgcctg atggcggtaa
    57421 aggttgatct tcacactcca gactatcggc agggcaacgt aatcgtacat gcatatgcgc
    57481 gcgatgctgg aaccagggtc gcactttgcg caaccagtcg cgatcggtgc ccgcatcaag
    57541 gcaaagttgt tgtttaatcg ccggattaac aaaaatgcgc gtgacgtctt tgtcctgggc
    57601 ggcgagtttg atcaagctga aaatttctgg cttccacagc gtggagacaa cgtgtttacc
    57661 gtcgcgggaa actaagtcca gtgcttgcgg gcgcaagagc tgcgcggagg tccagcgagt
    57721 tttcggcagt tgcagaaaga tatcgacatc cagtccggtc tggtggctgg catgaccgcc
    57781 gttgaaacgc ccaccagcgg gcatccccat atcgccaatc agcaccgtac ccatgcccag
    57841 attgctcacc tggctactca gacgctggat aaacatcacc agatccgggt gaccgaaata
    57901 gcgacgctga tcggtacgca tgacctgata atgttcggac tgtatcggca acgtgtcagc
    57961 gccgacaata cagccattag aaaaactgcc tatcgattgt gcgctacccg gcacaggttg
    58021 ggttattttt tgccacggcg tcgctgccag gctagcacta ctggcaagca gagccagcag
    58081 cgcaatcgcg gttttgttca ttttttacca gcgtggaata tcagtcttca catcggcatt
    58141 ttgcgcccgc tgccgtaaca ggtgatccat taaaacgatc gccagcatcg cttcagcgat
    58201 cggcactgca cggatcccga cacagggatc gtgacggcct ttggtgatca tctcaacttc
    58261 ttcgccaaag cggttaatag tgcgccccgg cacggtaatg ctggaggtcg gtttcagcgc
    58321 catatgagca atgatttgct gcccgctgct gataccgccg aaaatgccgc ccgcatggtt
    58381 gctctggaaa ccgtctttgg tgatttcgtc gcggttctgg ctgccgcgca gcgccactac
    58441 gtcaaaaccg tcgccaattt ccacgccttt caccgcgttg atgctcatca gcgcatgggc
    58501 gatgtcggca tccaggcggt caaagaccgg ctcgccaagt ccggcgggga caccactggc
    58561 aacaacggtg actttcgcgc cgatggagtc gccctctttt ttcagcgcgc gcatcagttc
    58621 atctaacgcg tcgattttgt cggggtccgg gcagaagaac ggattttgtt caaccagcga
    58681 ccagtctttg atttccagcg gaatgtcgcc catctgggtc aggcagccgc ggatttcaat
    58741 accaaatttc tcggcgagat attttttggc aatcgcccct gccgccacgc gcatggcggt
    58801 ttcgcgggcg gaagaacgtc caccgccgcg ataatcgcgc agaccgtatt tttgttcgta
    58861 ggtgtaatca gcatggcctg gacggaagac gtctttaatt gcgctgtaat cctgagaacg
    58921 ctggtcggta ttttcgatca acaagccaat gctggtgcca gtagtaacgc cttcaaaaac
    58981 accggagagt attttgacct gatccggctc gcggcgctgg gtggtatagc gcgatgtccc
    59041 agggcgacga cgatcaaggt catgttgcag gtccgcttcc gtcagcggaa tgcctggcgg
    59101 aacaccatcg acgatgcagc cgagcgccag cccgtgcgat tcgccgaagg tggttacgcg
    59161 aaagagttgt ccaattgtgt ttccagccat cacggctccg ttattgttgt gtttgcgtgt
    59221 ttacttaatc tttataaatc gcgaaatgtt ctcgtgcggc aataagctgc tctttagtga
    59281 gcataaacac accatcgccg ccgttatcaa actccagcca ggtgaacgga acatcgggat
    59341 attgttccat aagatgtacc atgctgttgc ccacttcaca aatcaacacg ccatcatcag
    59401 caaggtaatc tgccgcgtta ccgagaatgc gacgcgtcag tttcaggccg tcggtgccag
    59461 atgccaggcc cagttccggc tcgtgacggt attcgtttgg caggtcggac atatcttccg
    59521 cgtcgacata cggcgggtta gtgacaatca gatcgtattg cactttcggc aaatcacgga
    59581 acagatcgga acgaatcgga atgacgttgt ggatcagacc gtgttcttcg atgttctgtt
    59641 cggcaaccgc cagcgcgtcg ggagagatat ctaccgcgtc gacttccgct tccgggaagg
    59701 cataagcaca ggcaatggcg atgcagccgc taccggtaca catatctaaa atatgctgcg
    59761 gttgcttgct gatgagtccg gcaaatttat tgttgatcag ttcaccaatc ggcgagcgcg
    59821 gcaccagcac gcgttcatcg acgtaaaatt catgaccgca gaaccaggct ttgttggtca
    59881 ggtaagccac cggaatgcgc tcgttgacgc ggcggatcac gcgttcaaca atacggtgtt
    59941 tttcgctgga ggtcagacgc gcggtgcgca tatcttccgg gatatccagc ggcaggtaga
    60001 gcgatggtaa caccagctgt acggcttcat cccacgggtt atcggtgccg tgaccgtacc
    60061 agatatttgc cgcgctgaag cggcttaccg accagcgcaa tatgtcctga atggtttgca
    60121 gctcatttac tgcttcatca acgaaaattt tatccacgta ttcctccagg gcatgatcgc
    60181 aataatttcg gcggctagtt tgccatgaag atgacgataa atcagcattc acgcgcggtg
    60241 agtgaggaaa aatacgttta aaacgatcga ttgtgctacg agtcgggtaa actgtaggaa
    60301 aattagaaat agagacagat aaatgaaaaa gaaaacaaca cttagcgagg aggaccaggc
    60361 tctgtttcgc cagttgatgg cggggactcg caagattaag caggacacga ttgtccaccg
    60421 accgcagcgt aaaaaaatca gcgaagtgcc ggtgaaacgc ttgatccagg agcaggctga
    60481 tgccagccat tatttctccg atgagtttca gccgttatta aataccgaag gtccggtgaa
    60541 atatgttcgc ccggatgtca gccattttga ggcgaagaaa ctgcgccgcg gcgattattc
    60601 accggaattg tttttggatt tacacggtct gacgcagctg caggccaagc aggaactggg
    60661 cgcgttgatt gccgcctgtc gccgcgaaca tgtgttttgt gcctgcgtga tgcatgggca
    60721 cgggaagcat attttgaagc aacaaacacc gctgtggctg gcacaacatc cgcatgtgat
    60781 ggcatttcac caggcaccga aagagtacgg cggtgatgcg gctttgttgg tgttgattga
    60841 agtggaagag tggttgccgc cggagttgcc ctgaatgctg gcagcaaatg ccggagcagc
    60901 gtaaacgcct tatccggcct acaaatatgt gataattcaa taaattgcaa ttcacttcgt
    60961 aggcctgata agacgcatca gagcaatact acgaacttca gttcattcaa tacatactga
    61021 aaacgacacg tcgaatcaat cggttgccca ctactggtat tcacattcac cacaatatcg
    61081 tgtagtactt tatccgttac cggatgacga gttggcgtaa ccgttaccgt tgatttatca
    61141 caaccgtaag aggtaaaggt taacaattct gataccgcgc ggcagtatgc tcctctgcca
    61201 tcattatcag aaaaattact gccatcacag agtctgccgc cttccatcag gtaggtcgaa
    61261 agcgtggtgg tcactgttga ccccgtggac tgaattcgta acgccttatt atggctgaca
    61321 taattttcac tccaggagat atcgtatgaa gcgaccggta tcgttgggca tattgtgtta
    61381 tgcactgtag gggccgtata gcttccaaat ccgggtaaat ctgtatagga ataaaaggat
    61441 gcgatccaag ggcaaccata atcatcagat gcaacggtaa ttttaaacgt catatatgca
    61501 caacgaaaaa ttttcgtatc tatgccaggg caatttgagt tattaattgt atcaaagtca
    61561 gtcagtacca aagttggagc tgaacccgac ccagaccagg aaataacccg caacgtatcg
    61621 cctttatacg caaaagaatc atatttccca tatgcaacct cctttggcgt tatcaccgta
    61681 acattgaaaa cactgctcgc tacagcaccg gttaccttgt ccatggcata ataaactctc
    61741 acactgtcaa aactcgcact cataagttta tctccagcat aactcttaga tgcgccgaca
    61801 acactcagaa cgagcagcat taggctcgcg aaaattatgg tccatttttt catttcagaa
    61861 atactcaaag gttgccgtca ctgtggcact aaagtctccg gacgttacat ctcgcccgct
    61921 ctttgcctgt aaccaggtat taaaatttag gctgttattt ccctgattca gcgggagcgt
    61981 gacgcctgtt gtattgttga ttggcaccga tgtgcctgcc gcagtttcaa taccaatccc
    62041 agcgccggat gctgatgacg aggagtcaaa ggccagaaag ccgggtagag cactatcttc
    62101 tgttcctgtc aacgttactt tgacattaaa caatgtagaa ctatgacaat ctttcaattg
    62161 aaacaccaat ggcactcgct ctgactgccc aaggttcatt aaatcactgg cggcaatggc
    62221 aggaaaatgt acttctgcca actggctacc attaatcact ggcgtacacg atttactgat
    62281 taaattgccg gtaaagtgca aattatctcc tgctattgtg gttccactgc ccagcaacat
    62341 caggctacta ataaaaaaca acgtacgctt tttcttcatt ggtaatctac ctgtaacgtg
    62401 gcccaggcct caaagtccgc ctctggcaga atgacaccac tcttttttac tggcacagcc
    62461 gttaacaccg gtaaatcggt ttcattcacc acaagtggcg tattgatcgt aaaactctgc
    62521 cctgcctgtt tgagctgaat gcctaaacca gtgatattgc tgcttactgc agcattatca
    62581 aaatcgcttg gtgtgccact ccagctcaag gtcatttcca ttaatgggtc gcgaaccgcc
    62641 gtatcgcagg taatggaata cggcacggtt tgctcataat tgctgccatt aatgtcatca
    62701 ataagcacat cctgaaaagt tatttcaatg gtttgagcgt tgctaatcgt gcagttaggc
    62761 ggctgcacta aataaccgtg aaaagagacg tttgagagtg ccatcgagca aaagccccac
    62821 agcaaaataa gcgagatccg tttcattggt aatccaccac aattgtggca ctggcgttaa
    62881 attcagctgc cgctaacgtt gtaccactct gtttcaccgg cacagcttcc agaacgggaa
    62941 ctcccgagga aaaattaaat ggcaaccatg agccagaggt taagtcgagt attgtgtgat
    63001 cactactttt ctgaacccga ataccaaacc caggaatacc cgtgctgata actgtttcac
    63061 cgttaatcac gaccgttgtc gcctgcatct gtagacgcag gtcatccatt gccagattgt
    63121 tacaaacaag cgagtagtca atgggacgtt tataatcaac cctattgatc ttattaatga
    63181 atacattacc aaactcaact gccgcaccat ttaccgtaca aggaggaggg gcatcgacaa
    63241 gcacccgtaa attcatactt actgacttac tcgctgcaac tgcaaagccg ctaccaacac
    63301 aacatatcag taacccagtt accgcccaga cagaacaaga tcctttcata ctggctctcc
    63361 cggcctcagc ctttgcggct ctgctcttca tcgacctagc aactgttacc gttgcaagtg
    63421 aaaaagagcg gcatccgagc gccatagtcg ttgacatagg tcagtaccgg cacgctaccc
    63481 atcttcacat tcagcggtgc agaggttttc ggcgacatca ctattggcga gaaaccggct
    63541 gccggattgc catttttctg tgtgctggca ttactgaaaa tgacgtaata cgacgtcggg
    63601 ttattgacgg taaattcctg accgttgcgg gttaaagtaa tcttatattg ctaggggttt
    63661 ttcatgctga cgttctccag cgccttcggt cgccagaaca acttaatacg cgtttgtagt
    63721 gctatttgca gcgtgttggc tttgtttgat tttggcggga tctcacgcac gttgaagtag
    63781 aacaggcttt cacgatcggc aggcaatttg ttgatatccg gcattccctg cacttttacc
    63841 tgcccgttca tcattgaatc gatgcgttgc accggtggca gaacagttaa cggtgaactg
    63901 atcttgttac ccttttcatc ttcgatccag ctttgggcca gataaggcag tttcggatcg
    63961 ttattgcgca acgtcacgct gattgatttg tcgctttcat taaatatcaa acgggtacga
    64021 tctggcgtta cagaagcctg ggcagacaaa ctggcagcgc taagcagtaa cgctagcgtc
    64081 gtagcaccca atttggcaga gcaaagtaaa tcagacatgg atcattcctt aatcacgtta
    64141 ttggttagct gaaacagaaa cagggggatt ggtgggtgtc acctgttgcg tctgctcctg
    64201 aatgacaggc tttatgtcat caggaacaac cggcgcgacg ttgcctttat gctggcatgg
    64261 cagtaacaag ccgttaaaca gatcggcagg cagcgggtcc ggcaggttga tatcgcaatg
    64321 cgcaacacca ctccagaaca cactcatgtg ttcgccaggt ttcactccag ccagataaac
    64381 attgccgtca tcatcgacaa ggcccactgt ctgctcgtta tcatttttta cttctgcgcc
    64441 aaacggagga tggctgccgt cttgtaaacg cagcaccgcc atcgcttttt gaccactgat
    64501 gacggcaaat ttgcggtagc caatcgcccc ttcagttagc gtggcttgta ccaccgactg
    64561 ggttgcttcg gcgttttccg gcagtttgtt gaggtcgata tacgcctgat tgcggtaata
    64621 gttattgacg tcagaaacga cggctttacc aaacatattg gtataaacag cagcaccgtt
    64681 accttctacc ggaacatcgg caacgccatc ggcatcaatc aacaagcgtg tcccgcccat
    64741 attctgggta cggtgaagtg cgccaccgtg ggcagtaagc gttgcgccgc cctgtaacga
    64801 caagcccgca gaggtgtatt gcccttcatg gtagttcgca ctgaggtcaa cctgcgccag
    64861 cgaaccatca tggctgtaat agccgtcaac gctggtgtgt ttgtcactgg tgccgacgtt
    64921 caactgatag tgagtcgcgt catcgacacg gctgaaataa ccgacctgac tgctgtccgt
    64981 cccactgcca tagtaccgtt atagctaacg gtactgttgt cgccccacgg catactgagc
    65041 gaaatgtaca tgcctttgtc ggcctggttg tcatactcgt aacggtagcc agtcatcgag
    65101 atgctgacat tacgaatact gcccatattg aaatagtgcg agagcatgat gttgtagttt
    65161 gtctgctcct cgcgatccca gtaggtatgg cgggtatagt tgagataaac cgaaacaccc
    65221 gcatcgcgga agttctggtt ataagtggcg gtgtacatct ctttgtcgtt gcccgtgcgt
    65281 accattccgc tgtcactggc atccagatac tcgctcatgg tcataaagtt ctcttccgag
    65341 aagcgatatc cagcaaaagt aacgcggctg ttgagctggt caaagtcttt ggaataactc
    65401 acacggaagg agttaccgtc cagcgaacct ttgccataag cggtgtcttt atccagtttg
    65461 gtgtgcgagt gagtaacatc aaacgcgacc gcgccgaatg tagacaaatc gcgaccgaca
    65521 ccaagcgccg cagactgata gttttcatct cccagtgcgc cgccatataa cgaccagccg
    65581 ttagcgatcc cccaggaagc ttcagcacca gaaaaaaatt caccctcgac atggtgtccc
    65641 cactcttgcg gacggcccat catgatctta tagcgaacct gacctggacg agtgaggtat
    65701 ggcatcgagg cggtgctgat gtcatattcc tgcacctggc cgttctgttc ttcaatgcga
    65761 atatgcaacg taccggagac agaatcacca agatcctgaa tacgaaacgg tccagccgga
    65821 acctgcgttt cgtaaatcac acgccccatc tggctgacgg tcacttttgc tgtggtgtgt
    65881 gccacgccgg aaatgtctgg cgcgtagccg cgcagattgg gaggcaacat ttgatcgtca
    65941 gtactgacac tgccaccaac atagttaaaa ccatcaaaaa tatcggatct gaggtaatcc
    66001 tcgcccagcg ccagtttggc ttttaatgat ggtaacgccc gcctggcata gtagcgactc
    66061 cactcccatt tttttgagtt tcatcgccgc tgaattcatc gtcatcatta ctgcgagtat
    66121 gttgatagtt agtctgccag tcagcacgca tacgccacgg ccctaggtta accccaaccg
    66181 tcccgttgcc gctgatctcg ttactatcat caccgccatt ttcttcgtgc cgggtttgtg
    66241 cgttgatgct gtagtccgcg acgatcccgg agatgccgtc atcccaacgt gaaggcggat
    66301 ccctgtcggg ataagtatat tcgaggtaag cctgtggcag tgaaatgact aatgcggact
    66361 ggcttaaatc agccttaatt tccatgcctt ccagttgacc ggatttcagg catttagcat
    66421 cgtggctcca ttgcagattt ttcgccacgt cttcttttaa accaaactgc gctaccagtt
    66481 ccggtgtcag acaagcatag cttttgctcg cgtcatcttc accagcatac cagtaaatat
    66541 cgtactcttc cgccaatggc tgtttattta gttgaacctg taaattgtat tttccgggct
    66601 caacatatcc ctggctggaa aaacgcttca tatcaatttt tgtgtcgcct tttaattcca
    66661 gaaaacggga atcaaactga atgtcgtctt cagcccagac actgctataa ctccctgaca
    66721 ttgccagcgc aatgcaccac ggaagaatcc gtaatcgaaa aagagaatgg tcaggcatac
    66781 acgtttctat ccgtttttat tcttgttcgg tcaaaataca tttaaatccc ccatagcagt
    66841 gtcgctatgg gggtatgcct tacaggtaag taatctggaa ggtggtattt gcttcaaaat
    66901 tacccagatc tggcgcatca gctgcgccga ccagccaggc gttaaagttc agtgtctgct
    66961 tcgctttgcc tttgttggta gaagtgtctt tcactatttt ctggtcaata cccatgccgc
    67021 ttttgtaaga ggtacccaga gagtcaccga tcgccaggct aacattgtta aatgccgcac
    67081 cggtatcggt attgaaaatg gtgtaataat tgccgctatt tgcagaagaa acggtaccag
    67141 taaaggtagt ggtcattgtt tcctgagtat caaatacgca atcttgcaga cgaatctgga
    67201 aacttttcgg cgtggtggtg ccgttatttt tcagtttttc ggcaccgata tcacccatat
    67261 caacttccag tttgtgatcg tccggaacga tagaacacac tgagtcttca atggtgccgt
    67321 aaaaacgtgc agtaccgttg ttacccgcag cgattgttgc cgtagaggtg aacacgccca
    67381 tcaccattgc cgcagcaata gctgttttaa caaacttact cattatttgt ccttaaacta
    67441 taaataatta aaaatataaa cattgcaata cattgattca gtcaatagcc aatgtttata
    67501 cccagccatt gaatacggca tgcgacccaa aaagaacagg tcgtagaaaa ttgtattcat
    67561 tggtgtgggg ccgcatagtg atgtttccac acccctgatc caatttaatt ttactaaaga
    67621 gtaaatgtag atttaattaa tatattgaca gaagaaaggt tatcttaagg a
//
LOCUS       DAJTSI010000003        66984 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000003 DAJTSI010000000
VERSION     DAJTSI010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66984
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.003"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(65..1069)
                     /gene="cra"
                     /locus_tag="OO327_000142"
     CDS             complement(65..1069)
                     /gene="cra"
                     /locus_tag="OO327_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR6016826.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1151..1237)
                     /locus_tag="OO327_000143"
     CDS             complement(1151..1237)
                     /locus_tag="OO327_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR6016827.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="OO327_000144"
     CDS             complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="OO327_000144"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6016828.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="OO327_000145"
     CDS             complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="OO327_000145"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR6016829.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPRATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3586..3630
                     /locus_tag="OO327_000146"
     CDS             3586..3630
                     /locus_tag="OO327_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6016830.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="OO327_000147"
     CDS             complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="OO327_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR6016831.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5388..5474
                     /gene="leuL"
                     /locus_tag="OO327_000148"
     CDS             5388..5474
                     /gene="leuL"
                     /locus_tag="OO327_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR6016832.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5567..7138
                     /gene="leuA"
                     /locus_tag="OO327_000149"
     CDS             5567..7138
                     /gene="leuA"
                     /locus_tag="OO327_000149"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR6016833.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            7138..8229
                     /gene="leuB"
                     /locus_tag="OO327_000150"
     CDS             7138..8229
                     /gene="leuB"
                     /locus_tag="OO327_000150"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR6016834.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8232..9632
                     /gene="leuC"
                     /locus_tag="OO327_000151"
     CDS             8232..9632
                     /gene="leuC"
                     /locus_tag="OO327_000151"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR6016835.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAALVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9643..10248
                     /gene="leuD"
                     /locus_tag="OO327_000152"
     CDS             9643..10248
                     /gene="leuD"
                     /locus_tag="OO327_000152"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR6016836.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10297..11475)
                     /gene="setA"
                     /locus_tag="OO327_000153"
     CDS             complement(10297..11475)
                     /gene="setA"
                     /locus_tag="OO327_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCR6016837.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="OO327_000154"
     CDS             complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="OO327_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR6016838.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11797..13452
                     /gene="sgrR"
                     /locus_tag="OO327_000155"
     CDS             11797..13452
                     /gene="sgrR"
                     /locus_tag="OO327_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR6016839.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13486..13585
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13616..14599
                     /gene="thiB"
                     /locus_tag="OO327_000156"
     CDS             13616..14599
                     /gene="thiB"
                     /locus_tag="OO327_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR6016840.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14575..16185
                     /gene="thiP"
                     /locus_tag="OO327_000157"
     CDS             14575..16185
                     /gene="thiP"
                     /locus_tag="OO327_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR6016841.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16169..16867
                     /gene="thiQ"
                     /locus_tag="OO327_000158"
     CDS             16169..16867
                     /gene="thiQ"
                     /locus_tag="OO327_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR6016842.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16981..17745)
                     /locus_tag="OO327_000159"
     CDS             complement(16981..17745)
                     /locus_tag="OO327_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6016843.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17831..18709)
                     /gene="araC"
                     /locus_tag="OO327_000160"
     CDS             complement(17831..18709)
                     /gene="araC"
                     /locus_tag="OO327_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR6016844.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAMNLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            19048..20748
                     /gene="araB"
                     /locus_tag="OO327_000161"
     CDS             19048..20748
                     /gene="araB"
                     /locus_tag="OO327_000161"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCR6016845.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20759..22261
                     /gene="araA"
                     /locus_tag="OO327_000162"
     CDS             20759..22261
                     /gene="araA"
                     /locus_tag="OO327_000162"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR6016846.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22461..23156
                     /gene="araD"
                     /locus_tag="OO327_000163"
     CDS             22461..23156
                     /gene="araD"
                     /locus_tag="OO327_000163"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR6016847.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGTKAYYGQ"
     gene            23231..25582
                     /gene="polB"
                     /locus_tag="OO327_000164"
     CDS             23231..25582
                     /gene="polB"
                     /locus_tag="OO327_000164"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR6016848.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLIPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25747..28653
                     /gene="rapA"
                     /locus_tag="OO327_000165"
     CDS             25747..28653
                     /gene="rapA"
                     /locus_tag="OO327_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR6016849.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28665..29324
                     /gene="rluA"
                     /locus_tag="OO327_000166"
     CDS             28665..29324
                     /gene="rluA"
                     /locus_tag="OO327_000166"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR6016850.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="OO327_000167"
     CDS             complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="OO327_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR6016851.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30511..32865
                     /gene="lptD"
                     /locus_tag="OO327_000168"
     CDS             30511..32865
                     /gene="lptD"
                     /locus_tag="OO327_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR6016852.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32918..34204
                     /gene="surA"
                     /locus_tag="OO327_000169"
     CDS             32918..34204
                     /gene="surA"
                     /locus_tag="OO327_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR6016853.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            34204..35193
                     /gene="pdxA"
                     /locus_tag="OO327_000170"
     CDS             34204..35193
                     /gene="pdxA"
                     /locus_tag="OO327_000170"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR6016854.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLTIKMIVNTQ"
     gene            35190..36011
                     /gene="rsmA"
                     /locus_tag="OO327_000171"
     CDS             35190..36011
                     /gene="rsmA"
                     /locus_tag="OO327_000171"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR6016855.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            36014..36391
                     /gene="apaG"
                     /locus_tag="OO327_000172"
     CDS             36014..36391
                     /gene="apaG"
                     /locus_tag="OO327_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR6016856.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36398..37240
                     /gene="apaH"
                     /locus_tag="OO327_000173"
     CDS             36398..37240
                     /gene="apaH"
                     /locus_tag="OO327_000173"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR6016857.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37318..37797)
                     /gene="folA"
                     /locus_tag="OO327_000174"
     CDS             complement(37318..37797)
                     /gene="folA"
                     /locus_tag="OO327_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR6016858.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="OO327_000175"
     CDS             complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="OO327_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR6016859.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSGLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="OO327_000176"
     CDS             complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="OO327_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR6016860.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40421..41815)
                     /locus_tag="OO327_000177"
     CDS             complement(40421..41815)
                     /locus_tag="OO327_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6016861.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="OO327_000178"
     CDS             complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="OO327_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCR6016862.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42158..43444)
                     /locus_tag="OO327_000179"
     CDS             complement(42158..43444)
                     /locus_tag="OO327_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6016863.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43495..44436)
                     /locus_tag="OO327_000180"
                     /pseudo
     CDS             complement(43495..44436)
                     /locus_tag="OO327_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44451..45221)
                     /locus_tag="OO327_000181"
     CDS             complement(44451..45221)
                     /locus_tag="OO327_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCR6016864.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45577..47091
                     /gene="caiT"
                     /locus_tag="OO327_000182"
     CDS             45577..47091
                     /gene="caiT"
                     /locus_tag="OO327_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCR6016865.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLAGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            47122..48264
                     /gene="caiA"
                     /locus_tag="OO327_000183"
     CDS             47122..48264
                     /gene="caiA"
                     /locus_tag="OO327_000183"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCR6016866.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48393..49610
                     /gene="caiB"
                     /locus_tag="OO327_000184"
     CDS             48393..49610
                     /gene="caiB"
                     /locus_tag="OO327_000184"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCR6016867.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49684..51237
                     /gene="caiC"
                     /locus_tag="OO327_000185"
     CDS             49684..51237
                     /gene="caiC"
                     /locus_tag="OO327_000185"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCR6016868.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51346..52131
                     /gene="caiD"
                     /locus_tag="OO327_000186"
     CDS             51346..52131
                     /gene="caiD"
                     /locus_tag="OO327_000186"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCR6016869.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            52137..52727
                     /gene="caiE"
                     /locus_tag="OO327_000187"
     CDS             52137..52727
                     /gene="caiE"
                     /locus_tag="OO327_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCR6016870.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52813..53208)
                     /gene="caiF"
                     /locus_tag="OO327_000188"
     CDS             complement(52813..53208)
                     /gene="caiF"
                     /locus_tag="OO327_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCR6016871.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(53470..56691)
                     /gene="carB"
                     /locus_tag="OO327_000189"
     CDS             complement(53470..56691)
                     /gene="carB"
                     /locus_tag="OO327_000189"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR6016872.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56709..57857)
                     /gene="carA"
                     /locus_tag="OO327_000190"
     CDS             complement(56709..57857)
                     /gene="carA"
                     /locus_tag="OO327_000190"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR6016873.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(58313..59134)
                     /gene="dapB"
                     /locus_tag="OO327_000191"
     CDS             complement(58313..59134)
                     /gene="dapB"
                     /locus_tag="OO327_000191"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR6016874.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(59301..60215)
                     /gene="rihC"
                     /locus_tag="OO327_000192"
     CDS             complement(59301..60215)
                     /gene="rihC"
                     /locus_tag="OO327_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR6016875.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(60281..61231)
                     /gene="ispH"
                     /locus_tag="OO327_000193"
                     /gene_synonym="lytB"
     CDS             complement(60281..61231)
                     /gene="ispH"
                     /locus_tag="OO327_000193"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR6016876.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(61233..61682)
                     /gene="fkpB"
                     /locus_tag="OO327_000194"
     CDS             complement(61233..61682)
                     /gene="fkpB"
                     /locus_tag="OO327_000194"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6016877.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61769..62263)
                     /gene="lspA"
                     /locus_tag="OO327_000195"
     CDS             complement(61769..62263)
                     /gene="lspA"
                     /locus_tag="OO327_000195"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR6016878.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(62263..65079)
                     /gene="ileS"
                     /locus_tag="OO327_000196"
     CDS             complement(62263..65079)
                     /gene="ileS"
                     /locus_tag="OO327_000196"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR6016879.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(65122..66063)
                     /gene="ribF"
                     /locus_tag="OO327_000197"
     CDS             complement(65122..66063)
                     /gene="ribF"
                     /locus_tag="OO327_000197"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR6016880.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(66071..66289)
                     /locus_tag="OO327_000198"
     CDS             complement(66071..66289)
                     /locus_tag="OO327_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR6016881.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            66392..66655
                     /gene="rpsT"
                     /locus_tag="OO327_000199"
     CDS             66392..66655
                     /gene="rpsT"
                     /locus_tag="OO327_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR6016882.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        16248 a        18240 c        17242 g        15254 t
ORIGIN      
        1 atgtccagtc ccgtactcta cgcgccagag ggaaattcac ctggcgcgta tttttgttcg
       61 cggattagct acggctgagc acgccgcggc gatagagatt acgtttaatg cgcgttaaac
      121 caggttttgg cttacgcggt tcgtccaggc ttgccaggac aatctccagc acacgctctg
      181 cgacatcgcg gtgacgttga gccactgcca gcaccggaca ctgtaagaag tcgagcagtt
      241 cgttatcgcc aaaggtggca attgccaggt cagaaggcag tttgccgtcg cgacgcagcg
      301 tgacatccat cactccttgc aacaacgcaa acgacgttgt gaacagcgcc tgcggcatcg
      361 gatgcgtttc cagccatttt tcgaataact gggcagccgc ctcccgctca tagctgttgg
      421 catacaggaa atgcacttcg cgcggatcat ctttccaagc agtacggaaa ccttgttcac
      481 gcaggaagct gacagaaagc tccggcagcg cgccaagata aagcaccgtc tcggcgggaa
      541 acttacgtaa ctcttccgcc agcatttcgg catcatcctg atcggcacca accacgctgg
      601 tgaaatgttc acgatcgagg gcgcggtcca gcgcgacaat cgggaacggg tcgttagccc
      661 agcgttgata aaaaggatgc tcaggtggca acgacgtcga aacaataatg gcatcaacct
      721 gacgctgtaa aaggtgctca atgcagcgca tttcgttgtc tggctgatct tctgagcagg
      781 caatcagcag ttgataaccc cgttgccgcg cctggcgttc aagatagtta gcgatgcggg
      841 tatagctggt gttctccaga tcggggatca caagaccaat agaacgtgtg cgtccagcac
      901 gaagcccagc tgccacggcg ttcgggtggt aattgtgctc acgcaccaca gccatgactt
      961 tttcaacggt tttgtcgctc acacggtatt gcttcgcttt gccgttaata acatagcttg
     1021 cagtggtccg cgacactccc gccagccgag cgatttcatc cagtttcaca attgcccctt
     1081 gcgtaaaatg taaaaaccat agccattgta ctggtatggg ttaaattcat taacatctaa
     1141 gcgcagaaaa ttaactgcgg caactgcttt tattccaggt tcccactgat ttcgcaaaaa
     1201 aagcccaacg tgacatgttg ggctgcaaat tgcgcactga gatcattctc aacgcattat
     1261 tttatcgccg cgcgaaagtc cgaccacacc agagcgagca acttccacaa ttttcgccac
     1321 atcgcgaatc gatgctaaaa atgcatcaag cttatcgctg gtgcctgcta attgaacggt
     1381 ataaagcgag ggggtgacat cgataatttg cccacggaat atttccgtat tacgtttcac
     1441 ttcgtcacgc ccgtaaccgc tggcctgaat tttcaccagc atgatttccc gctcaacatg
     1501 cgcgccctgc cccaactcac tcacgcgcaa gacatcgacc agcttgtgta attgcttttc
     1561 gatctgctca agtacttttt catcgcccac ggtctggatg gtcatacgcg ataatgtcgg
     1621 atcgtcggtt ggcgcaacgg tcaggctttc aatgttgtag cctcgctggg aaaaaaggcc
     1681 aatcacgcgg gataacgcgc ctgattcatt ttcgagtaag actgataata tccggcgcat
     1741 aatcaggttc tctccgtttt gcttaaccac atttcatcca ttccgccccc gcgaatctgc
     1801 atcgggtaga cgtgctcact gccatcgacg gtaacatcaa caaacaccag gcgattattg
     1861 cgcacctgtt ccagcgcctc gctaagtttg ctttccagct catgcggatg agagatctgg
     1921 atcccgacgt gaccataggc ttccgccaga cggacgaaat cgggtagtga ttgcatataa
     1981 gattgtgaat gacggccgga atagatcatg tcctgccact gtttcaccat ccccagatag
     2041 cggttattga gattcaccac cagtacgggc aactcgtatt gcaacgcggt agacagttcc
     2101 tggatgttca tctgaatact gccgtcgcca gtgacgcaaa ccacggtttc ttctggcaac
     2161 gccattttga cgcccagtgc cgcaggtaaa ccaaaaccca tcgtgccgag accgccggaa
     2221 ttgatccagc gacgcggttt gtcgaatgga taataaagcg cagcaaacat ctggtgctgc
     2281 ccgacatcgg acgtcacgta agcgtcccca ttagtcaacc gccaaagagt ctcgatcacc
     2341 gcctgcggtt taatcttttc actgtgagtg tcatatttca ggcactgaag agcgcgccac
     2401 tgttcaatct gctgccacca gtcgcggatc tcatccagcg gttgatgggc ggattcttgc
     2461 gacaggagtt caagcatttg ttcgaggacc tggcgagcat cccccacaat cgggatatcc
     2521 gcagtcacgg ttttagaaat ggaagtagga tcaatatcga tatgcagaac agtggcattt
     2581 gggcagtact ttgccagatt gttcgtcgtt cggtcatcaa atcgtacccc gacggcgaaa
     2641 atcacatccg cgttatgcat cgtcatattg gcttcgtagg taccgtgcat tcccagcatg
     2701 cccagtgcct gacgatgcgt tgccggaaac gcccccattc ccatcaatga ggaaacaacg
     2761 ggcagattca acgcctccac cgtttctttc aactgctgat ggcagcccgc catgattgcc
     2821 ccaccgccta cgtagacaac cggttttttt gccgctacca gcgtttgcag agcacgctta
     2881 atttgccctt tatgtccggt agtagtggga ttgtaagaac gcatactgac cgactcaggg
     2941 cagacatagg gtaatttgtt cgccggatta agaatatctt tcggtaaatc aacgaccact
     3001 ggtcctgggc gaccacttgc cgccagccag aaagcctttt tcagcacctg cggaatgtct
     3061 tccgtttgct taaccagaaa actgtgttta accaccggtc gcgaaatccc caccatgtcg
     3121 cactcctgaa aggcatcgta acctatcaac gaggtcgcta cctgcccgga aaggacaact
     3181 aatggaatgg aatccatata agcggtggcg atgccggtaa tcgcattggt cgcccttgga
     3241 cccgacgtta ccagcacgac gccgacttcc ccggtcgcac gcgccaggcc atcggccata
     3301 tgcaccgccg cctgctcatg acgaactaat acatgatcaa taccacccac ggtatgcaat
     3361 gcatcgtaaa tatcaaggac tgcgcctccg ggataaccga atacttgttt aacgccctga
     3421 tcgataagcg atcggacgac catctcggct ccagacaaca tctccatggc ctgcctcact
     3481 gtttgacgga aaaaatgtgt aaaacacatt aaccgtccga tcttgcttaa gcaattggca
     3541 gccagagaca taaacgcagg gtaataagaa taaacaggag gaaaggtgaa aagaaacgat
     3601 aaaatcctcc attacgaagg attaaattga taataaatcg gctgaatccc acaacttaca
     3661 gcaatttctc tattttttta attaatccta aaatagagag tccacacatt cagaataaaa
     3721 ttctacattc aataaaaaat gcagaataaa ccagacattc atgtctggcc tattctgcat
     3781 tcagttagcg tttgcaaatt gagactaatt gttcttccat ccactgatgg cctttatcgc
     3841 gcccggcagc ttcatgccaa gagagataac aggttctgct gttttgtttt aacggcagcg
     3901 gtaatacctg taattctaag gattcagcga actcttcagc cagccaacgc ggcgcaatag
     3961 cgaccaaatg cgtttgcgac accacgctaa gtacgctcat cattgccatg ccctgatacg
     4021 cgatactggc ttgcttatct accgtgtcat accaaggttg actaaatgac gcgaaacgat
     4081 cgagcgaaac cgccgcatgt tgttcgttat aaacatcatg tttcagtaac gggcctttaa
     4141 ttgttggatg atttttgctg gctaccagca ccatttcatc tttaaataat ggcacgctgg
     4201 taaattcagg acgatggaag tcttcataac taatcacaaa ctccgtttcc tgataacgca
     4261 gctgatgttc agtgttctga tttaatgaag acttgaacat aacatgtata tttggcgcaa
     4321 tctgctcaat gtgattataa atctgcgagg tcagaatgct gtctaacggg ctgcaaacac
     4381 aaagatgaaa tacacgttca ctgctcgcgg gttcaaaacc tgaaccaggc aattcatttt
     4441 gtactagttg caatgcctgg cgaactgaac caaaaagttg aaatgcgcga gcagtcggtt
     4501 gaataccacg gccataacga acaaaaagct cgtcattaaa catcaccttc aggcgtgcaa
     4561 cagcgttact gaccgcaggt tgcgacatac ccagaacatg agcggcacgg gtaatgtttt
     4621 gctcctgcat cacggcatcg aaaacggtta ataagttgag atcgaccatg cgtagctgtg
     4681 gcttgcttaa ctccgccgtc tctgaatgat ctgtttgtac ctctggcata cttaactcca
     4741 ctgtcacact taactccctt tcccttattg gaatgcagag aaataatcct gaagaatatg
     4801 atttatcatg catataaaat aagaaaaagc aaaatgagta aaattcggaa aacataaaga
     4861 tacgggttct gataaaaaca gaatccattg ccgtggaatg agtcatttac cacagcataa
     4921 taatccataa tgttttgttg cgaaaacaat ctaatcataa ctaccgcgaa tactcaatca
     4981 tctacaaaat ggattaaatg tgcttattta ataattaatt tatatattta atgcattaat
     5041 tcttaacatt aattgttcaa taatattcac caaatcaata tcaaaaaaaa tcgcaaaaca
     5101 tataattcaa tacaaatcat caggataggt tttgcaacgc gtgcattttg tccccttttt
     5161 cctcgttgat tagatgcaaa aatttatgct gaaatatgtc aaccgatgaa aagcgtcggt
     5221 agttaagcag aaattaatat cgcttacttt aaccaccgta gcacaattag ctaattttac
     5281 ggatgcagaa ctcacgctgg cgggacgttt ttattgcgtc agggttgaca tccgtttttg
     5341 tatccagtaa ctctaaaagc atatcgcatt catctggagc tgatttaatg actcacatcg
     5401 ttcgctttat cggtctacta ctactaaacg catcttcttt gcgcggtaga cgagtgagcg
     5461 gcatccagca ttaagccagc acgcagtcaa acaaaaaacc cgcgccattg cgcgggtttt
     5521 tttatgcccg aagcgaggcg ctctaaaaga gacaaggacc caaaccatga gccagcaagt
     5581 cattattttc gataccacat tgcgcgacgg tgaacaggcg ttacaggcaa gcttgagtgt
     5641 gaaagaaaaa ctgcaaattg cgctggccct tgagcgtatg ggtgttgacg tgatggaagt
     5701 cggtttcccc gtctcttcgc cgggcgattt tgaatcggtg caaaccatcg cccgccaggt
     5761 taaaaacagc cgcgtatgtg cgttagctcg ctgcgtggaa aaagatatcg acgtggcggc
     5821 cgaatccctg aaagtcgccg aagccttccg tattcatacc tttattgcta cctccccaat
     5881 gcacatcgcc accaagctgc gcagcaccct agacgaggtg atcgaacgcg ctatctatat
     5941 ggtgaaacgc gcccgtaatt acaccgatga tgttgaattt tcttgcgaag atgccgggcg
     6001 tacacccatt gccgatctgg cgcgagtggt cgaagcggcg attaatgccg gtgccaccac
     6061 catcaacatt ccggacaccg tgggctacac catgccgttt gagttcgccg gaatcatcag
     6121 cggcctgtat gaacgcgtac ctaacatcga caaagccatt atttccgtac atacccacga
     6181 cgatttaggc ctggcggtcg gcaactcact ggcggcggta catgccggtg cacgccaggt
     6241 ggaaggtgca atgaacggga tcggcgagcg tgccggtaac tgttcgctgg aagaagtcat
     6301 catggcgatt aaagttcgta aggatattct caacgtccac accgccatta atcaccaaga
     6361 gatatggcgc accagccagt tagttagcca gatttgtaat atgccgatcc cggcaaacaa
     6421 agccattgtt ggcagcggcg cattcgctca ctcctccggt atccaccagg atggcgtgct
     6481 gaaaaaccgc gaaaactacg aaatcatgac accagaatct attggtctga accaaatcca
     6541 gctgaatctg acctctcgtt cggggcgtgc ggcggtgaaa catcgcatgg atgagatggg
     6601 gtataaagaa agtgaatata atttagacaa tttgtacgat gctttcctga agctggcgga
     6661 caaaaaaggt caggtgtttg attacgatct ggaggcgctg gccttcatcg gtaagcagca
     6721 agaagagccg gagcatttcc gtctggatta cttcagcgtg cagtctggct ctaacgatat
     6781 cgccaccgcc gccgtcaaac tggcctgtgg cgaagaagtc aaagcagaag ccgccaacgg
     6841 taacggtccg gtcgatgccg tctatcaggc aattaaccgc atcactgact ataacgtcga
     6901 actggtgaaa tacagcctga ccgccaaagg tcacggtaaa gatgcgctgg gtcaggtgga
     6961 tattgtcacc aactacaacg gtcgccgctt ccacggcgtc ggcctggcca ccgatattgt
     7021 cgagtcctcc gccaaagcca tggtgcacgt actgaacaat atctggcgtg ccgcagaagt
     7081 cgaaaaagag ttgcaacgca aaactcaaca caacgaaaac aacaaggaaa ccgtgtgatg
     7141 tcgaagaatt accatattgc cgtattgccg ggagacggta ttggtccgga agtgatgacc
     7201 caggcgctga aagtgctgaa tgccgtgcgc aaccgctttg cgatgcgcat caccaccagc
     7261 cattacgatg taggcggcgc agccattgat aaccacgggc aaccactgcc gcctgcgacg
     7321 gttgaaggtt gtgagcaagc cgatgccgtg ctgtttggct cggtaggcgg cccgaagtgg
     7381 gaacatttac caccagacca gcaaccagaa cgcggcgcgc tgctgccgct gcgtaagcac
     7441 ttcaaattat tcagcaacct gcgcccggca aaactgtatc aggggctgga agcattctgt
     7501 ccgctgcgtg cagacattgc cgctaacggc ttcgacatcc agtgcgtgcg cgaactgacc
     7561 ggcggcatct atttcggtca gccaaaaggc cgcgaaggta gcggacaata tgaaaaagcg
     7621 tttgataccg aggtgtatca ccgttttgag atcgagcgta ttgcccgcat cgcgtttgaa
     7681 tctgctcgta aacgtcgcca caaagtcacc tcaatcgaca aagctaacgt gctgcaatcc
     7741 tctattttat ggcgtgaaat cgtcaacgag atcgccacgg aatacccgga tgtcgaactg
     7801 gcgcatatgt acatcgacaa cgccaccatg cagctgatta aagatccatc acagtttgac
     7861 gttctgctgt gctccaacct gtttggcgac attctgtctg acgagtgcgc aatgatcact
     7921 ggctcgatgg gcatgttgcc ttccgccagc ctgaacgagc aaggttttgg actgtatgaa
     7981 ccggcgggcg gctcggcacc agatatcgca ggcaaaaaca tcgccaaccc gattgcacaa
     8041 atcctgtcgc tggcactgct gctgcgttac agcctggatg ccgatgatgc ggcttccgcc
     8101 attgaacgcg ccattaaccg tgcattagaa gaaggcattc gcaccgggga tttagcccgt
     8161 ggcgctgccg ccgttagtac cgatgaaatg ggcgatatca ttgcccgcta tgtggcagaa
     8221 ggggtgtaat catggctaaa acgttatacg aaaaattatt cgacgctcac gtagtgtacg
     8281 aagccgaaaa cgaaaccccg ctgttatata tcgaccgcca cctggtgcat gaagtgacct
     8341 caccgcaggc gttcgatggt ctgcgcgccc acggtcgccc ggtacgtcag ccgggcaaaa
     8401 ccttcgccac tatggatcac aacgtctcta cccagaccaa agacattaat gcctgtggtg
     8461 aaatggcgcg catccagatg caggagctga ttaaaaactg caaagaattt ggcgtcgagc
     8521 tgtatgacct gaatcacccg tatcagggga tcgtccacgt aatggggccg gaacagggcg
     8581 tcaccttgcc ggggatgacc attgtctgcg gcgactcgca taccgccacc cacggcgcgt
     8641 ttggcgcact ggcctttggt atcggcactt ccgaagttga acacgtactg gcaacacaaa
     8701 ccctgaaaca gggccgtgcg aagaccatga aaattgaagt ccagggcaaa gccgcgccgg
     8761 gcattaccgc aaaagatatc gtgctggcaa ttatcggtaa aactggcagc gcaggcggca
     8821 ccgggcatgt ggtggagttt tgcggcgaag caattcgtga tttaagcatg gaaggtcgta
     8881 tgaccctgtg caatatggcg atcgaaatgg gcgcaaaagc cgctctggtt gctcctgacg
     8941 aaaccacctt taactatgtc aaaggccgtc tgcatgcgcc gaaaggcaaa gatttcgacg
     9001 acgccgttgc ctactggaaa accctgcaaa ccgacgaagg cgcaactttc gataccgttg
     9061 tcactctgca agcagaagaa atttcaccgc aggtcacctg gggcaccaat cccggccagg
     9121 tgatttctgt gaacgacaat attcccgatc cggcttcgtt tgccgatccg gttgaacgtg
     9181 cgtcggcaga aaaagcgctg gcctatatgg ggctgaaacc gggtattccg ctgaccgaag
     9241 tggctatcga caaagtgttt atcggttcct gtaccaactc tcgcattgaa gatttacgcg
     9301 cggcagcgga gatcgccaaa gggcgaaaag tcgcgccagg cgtgcaggca ctggtggttc
     9361 ccggctctgg cccggtaaaa gcccaggcgg aagcggaagg tctggataaa atatttattg
     9421 aagccggttt tgaatggcgc ttgcctggct gctcaatgtg tctggcgatg aacaacgacc
     9481 gtctgaatcc gggcgaacgt tgtgcatcca ccagcaaccg taactttgaa ggccgccagg
     9541 ggcgcggcgg gcgcacgcat ctggtcagcc cggcaatggc tgccgctgct gctgtgaccg
     9601 gacatttcgc cgacattcgc aacattaaat aaggagcaca ccatggcaga gaaatttatc
     9661 aaacacacag gcctggtggt tccgctggat gccgccaatg tcgataccga tgcaatcatc
     9721 ccgaaacagt ttttgcagaa agtgacccgt acgggttttg gcgcgcatct gtttaacgac
     9781 tggcgttttc tcgatgaaaa aggccaacag ccaaacccgg acttcgtgct gaacttcccg
     9841 cagtatcagg gcgcttccat tttgctggca cgagaaaact tcggctgtgg ctcctcgcgt
     9901 gagcacgcgc cctgggcatt gaccgactac ggttttaaag tggtgattgc gccgagtttt
     9961 gctgacatct tctacggcaa tagctttaac aaccagctgc tgccggtgaa attaagcgat
    10021 gcagaagtgg acgaactgtt tgcgctggtg aaagccaatc cggggatcca tttcgacgtg
    10081 gatctggaag cgcaagaggt gaaagcggga gagaaaacct atcgctttac catcgatgcc
    10141 ttccgccgcc actgcatgat gaatggtctg gacagcatcg gcctgaccct gcagcacgac
    10201 gacgctattg cgtcttatga agcaaaacaa cctgcgttta tgaattagac cccttgcccg
    10261 gtcaaatgac cgggctttcc gctatcgtcc acgtcatcaa acgtctttaa cctttgcggt
    10321 taaaaataat gcgacaacag aaataatcgc aattacccag tagacagcaa agtgccccca
    10381 actttgtgca attgctccct gaataacacc agccagaatt accccggtag agatagtgtt
    10441 ggtaaataag gtggtagctg cccccgctct tccaggcatt aaatcctgaa accatagcat
    10501 cccaataccc gcaacaatgc cgataaatac agcgttaaaa agttgcagcg tcatcaacgc
    10561 catacggcta tgaaagaaaa tcaatccagt gtaaaacagc actcctgcca ccactgcaat
    10621 gaccatcatt cgccgcttac cataacgttt gacatagtag ccagccagaa tcattgctgg
    10681 tatttccagt ccagctgccg tccccatcag gaaacccgcc agtttgtctg gcaatcctaa
    10741 atcgctactg atccacaatg gcatatcaat aatgtacata gtgttgcagg tccacattaa
    10801 cgtcgaggcg acaaataaca tccgtacgtt actatcctgc cagtcacctt gcattgataa
    10861 agcattttcc gacggcagtt ctacccgcgc cacagacaga agcataaatg caatcaatac
    10921 cagactgagt gtgaatatcc cggcggcaat cgaaaacatc actgtaaagc cgtaattcaa
    10981 cgccagcata aaggccagcg gtggaccgat tacccatgcc agagaaagct gcgcacgcat
    11041 caccgagcta aacatcacca cttctcgcgc cgagttatcc gcgtattccc gcgccagagc
    11101 aaataactgt ggcattgccg tattggccag agatgccaga agcacaccac aggtgataag
    11161 cgtcagataa tgacgattaa atgcaaacag tagcgcattg ccaatagcca tcaaacagca
    11221 aaatataatc agttttcgcc gatcgccctg actgtcagaa cgttttgcca accagaggct
    11281 tacgccgatc ccagcaatag cattcaccgt ataaaagagt ccgatccaga aaggttgcgc
    11341 gccaacctca cgactcagaa ataagctcaa tgtaggagcc tgtagcgccc cggccacccc
    11401 catcataaaa gcgaccagca taaatgccgc gtaaacaccg ttcatacggc gagccatcgt
    11461 cattatccag atcatacgtt ccctttttag cgcggcgaga ataaaaaaaa ccagcaggta
    11521 taatctgctg gcgggtgatt ttacaccaat actcagtcac acatgatgca ggcaagtcaa
    11581 ctttcagaat tgcggtcatc ccactgcatc agtccttcaa gcatggttaa tcgttgtggg
    11641 acgcttaacc aacgcaacca gcacaacttc gctgtcgcgg taaaatagtg ctgataaaac
    11701 tgacgcatgg ggcacctcct tgcttcatcg ttaggaatta ttgacttaat atagggaaaa
    11761 taaaattgct gtcttttgcg caggagttcc ccttttatgc catctgctcg tctgcaacaa
    11821 cagttcatcc gcctgtggca atgctgcgag ggtaaatcgc aggacacaac gctcaacgaa
    11881 ctggcagcgt tattgagctg ctcgcgtcgt catatgcgta ccctgctcaa caccatgcag
    11941 gatcgcggct ggctgacgtg ggaagcggaa gtcgggcgcg gtaaacgctc gcgtctgaca
    12001 ttcctctata ccgggctggc gcttcagcaa cagcgggcgg aagacctgct ggagcaggat
    12061 cgtatcgacc aactagtgca gttggttggc gacaaagcga ctgtgcggca aatgctggtt
    12121 tctcatctgg gccgcagctt ccgccagggg cggcacatcc tgcgcgtgct ctactatcgt
    12181 ccgttgcgta atctgctacc tggcagcgca ttgcgccgtt ccgaaaccca tattgcccgg
    12241 caaatcttca gttcgctaac gcgcataaat gaggaaaatg gggaactgga agcagacatc
    12301 gcccaccact ggcagcaaat atcaccgctt cactggcgtt tctttttgcg tccaggagtc
    12361 cattttcatc atggtcgtga actggaaatg gacgacgtga tcgcctcttt aaaacgaatc
    12421 aatacgctgc cgctctattc gcatattacg gacattgtgt cgccgacgcc ctggacgctg
    12481 gatatccatc tcacgcagcc ggaccgctgg ttgccgttac tgctggggca agttccggcg
    12541 atgatcctgc cgtgcgaatg ggaaaccctc agtaactttg ccagccatcc tattggcacc
    12601 ggtccgtatg cggtgattcg caacagcacc aatcaactga aaattcaggc attcgatgac
    12661 ttcttcggtt accgggcatt aatcgacgaa gttaacgtct gggttctgcc ggaaattgcc
    12721 gacgagccag ccggtggtct gatgctaaaa ggtccacagg gcgagaaaaa agagattgaa
    12781 agccgcctgg aggaaggttg ctactattta ctgttcgaca gccgcaccca tcgcggggcg
    12841 aatcagcaag tcagggactg ggtaagctat gtgctttctc caactaatct ggtctatttc
    12901 gctgaggaac agtaccagca actgtggttc ccggcttatg gactgctccc ccgttggcac
    12961 catgcccgca ccataaagag cgaaaaaccg gctggcctgg aaagcctcac cctgaccttt
    13021 tatcaggatc acagtgagca tcgggtgatt gccgggatca tgcagcagat tctggcaagt
    13081 caccaggtca cgctggaaat caaagagatc agctacgatc agtggcatga aggagagatc
    13141 gagagcgata tctggcttaa tagcgccaac tttacgctgc cgctggattt ttcgctgttc
    13201 gcgcacttgt gcgaggtgcc gctgctccaa cactgtattc cgatcgactg gcaagccgac
    13261 gccgcccgct ggcgcaatgg cgaaatgaac ctggcgaact ggtgccagca actggtcgcc
    13321 agtaaagcaa tggtgccgct tatccaccac tggctgatca ttcagggaca acgcagtatg
    13381 cgcggcctgc gtatgaatac cctcggctgg ttcgatttta aatcagcgtg gtttgcgcca
    13441 ccggatccat gattgctggt agataacaaa atcactacac taacgccgtt ctcaacgggg
    13501 tgccacgcaa acgcgtgcgc tgagaaaata cccgtcgaac ctgatccgga taacgccggc
    13561 gaagggattt gaggctcctt ctcaagtcct ttgccactct tttttgaggt gcaaagtgtt
    13621 aaaaaaatgt cttcccctgc tgttgctgtg cacagcgccc gttttcgcta aacccgttct
    13681 gactgtttat acctacgatt ccttcgccgc cgactggggg cctggtccaa agatcaaaaa
    13741 agcttttgaa gccaactgta actgcgaact gaaactggtg gcgctggaag atggcgtttc
    13801 gcttctcaac cgtctacgga tggaaggcaa aaatagtaaa gccgatgtgg tgctggggct
    13861 ggataacaat ctgttagacg ccgccagtaa aaccggacta tttgccaaaa gcggtgtggc
    13921 agcggatgcc gttaacgttc ccggcggctg gaataatgac actttcgtcc cgtttgatta
    13981 cggctatttc gccttcgtct atgacaagaa caaactgaaa aacccgccgc aaagcctgaa
    14041 agaattggtt gagagcgatc agaactggcg ggtgatttat caggatccgc gtaccagcac
    14101 gccggggctg ggtctgctgt tgtggatgca aaaagtctat ggcgatgacg ccccacaagc
    14161 ctggcaaaaa ctggcgaaga aaaccgtcac ggtcaccaaa ggctggagcg aagcctacgg
    14221 cctgttttta aaaggtgaaa gcgatctggt actgagttac accacctctc cggcttatca
    14281 cattctcgaa gagaaaaaag ataactacgc cgccgcgaac tttagcgaag gtcactatct
    14341 gcaggtggaa gtcgccgccc gcaccgctgc cagcaagcag ccggagctgg cgcaaaaatt
    14401 cctccagttt atggtttctc cggcttttca gaatgcgatc ccaaccggta actggatgta
    14461 tccggtggca aatgtcacgc tgcctgccgg gtttgaacaa ttgaccaaac cggccaccac
    14521 gctggagttc acaccagctg aagtggcggc acaacgtcag gcatggatta gcgaatggca
    14581 acgcgccgtc agccgttaat tcccggctgg ttaattccag gtgtaagcgc cgccacgctg
    14641 gtggtagcgg ttgcgctcgc ggcgtttctc gccctgtggt ggaacgcgcc gcagggtaac
    14701 tgggtggcag tctggcagga cagctacctg tggcatgtgg tgcgcttctc cttctggcag
    14761 gcgtttctct cggcgctact ctctgtcgta cccgcgatat tcctcgcccg cgccctctat
    14821 cgcaggcgct ttccgggtcg gctggcgctg ttgcgtctgt gcgcaatgac cttgatcctc
    14881 ccggtgctag ttgctgtttt cggcattctt agcgtctatg gtcgtcaggg ctggctggca
    14941 tcgctctgcc aatcgctcgg tctggagtgg accttttcgc cctacggcct gcaaggcatt
    15001 ttgctggcgc acgtattttt taatctgccg atggcgagcc gcttattact ccaggcactg
    15061 gaaaacattc ccggcgaaca acgtcagctt gccgcccagc ttgggatgcg cggctggcat
    15121 tttttccgct tcgtcgaatg gccgtggcta cggcgacaaa tcccgccggt tgccgcgctt
    15181 atctttatgc tctgtttcgc cagcttcgcc accgtgctat cgctgggcgg cggtccgcag
    15241 gcaaccacta tcgagctggc tatttatcag gcgctgagtt acgactacga tcctgcccgc
    15301 gcggcgatgc tggcgctgct ccagatggtg tgttgccttg ggctggtgct gttgagtcag
    15361 cgactaagta aggccattgc gcccggcacc acgctgctgc aaggctggcg cgatccggac
    15421 gatcgtctgc atagccgcat ttgcgacacg gtgttaatag tgctggcgct gctgctgttg
    15481 ctgccaccgt tgctggcggt gatcgtcgat ggggtaaatc gccagttgcc ggaagtgctg
    15541 gcacaaccgg tgctgtggca ggcgctgtgg acctcgttgc gtattgcgct ggcggcaggt
    15601 gtattgtgcg tagtgctgac catgatgctg ctatggagca gtcgcgaact gcgggcgcgg
    15661 cagaaaatgc tggcgggtca ggcgctggag atgagcggca tgttgatcct cgccatgccg
    15721 gggattgtgc tggctaccgg cttcttttta ctgctcaaca acactatcgg cctgccacaa
    15781 tctgctgacg gcattgtgat tttcaccaat gcgttaatgg cgatccctta tgcgctgaaa
    15841 gtactggaaa acccgatgcg cgatatcacc gcccgctaca gtatgttgtg tcagtcgctg
    15901 ggcattgaag gctggtcgcg cttaaaagtg gtcgagctgc gcgccctgaa acgtccactg
    15961 gcgcaggcgc tggcttttgc ctgcgtgctg tcgattggtg attttggcgt agtggcgttg
    16021 ttcggtaacg atgatttccg taccctgccg ttttatctct accagcaaat tggctcctat
    16081 cgcagccagg acggcgcggt caccgcgtta attctgctac tactctgttt tctgctgttt
    16141 accgtgattg aaaaactacc ggggcgaaat gttaaaactg actgatatca cctggcttta
    16201 tcaccatttg ccgatgcgtt ttagcttaac ggtagaacgc ggcgagcagg tggcaatcct
    16261 cgggccaagc ggcgcgggta aaagtaccct gctgaatttg attgccggtt ttctgacgcc
    16321 agccagcggt tcgctgacta tcgatggtgt tgatcacacc acgacgccgc cgtcacgccg
    16381 tccggtgtcg atgctgtttc aggagaacaa cctgttcagc catctgacgg tcgcgcagaa
    16441 catcgggctg gggctaaatc cggggttgaa actgaacgcg gcacagcagg agaaaatgca
    16501 cgcgattgcc cgtcagatgg ggattgataa tttaatggcg cggttaccgg gcgagctttc
    16561 cggcggtcag cgacagcgcg tggcgttagc gcgttgtctg gtgcgcgaac agccgatttt
    16621 attgctcgat gaaccgttct ctgcgctcga tccggcgtta cgtcaggaga tgctgacgct
    16681 ggtgagcacg agctgccagc agcaaaaaat gacgctattg atggtgtcgc acagtgtgga
    16741 agatgcggcg cggatcgcca cacgctcggt ggtggtcgcc gacgggcgca tcgcctggca
    16801 gggtaagact gaagaattgt tgagcggtaa ggcgagtgct tcggcaatat tggggattac
    16861 gggttagtat gcactgtcag atgcggcgtg aacaccttat ccgacctacg gacagtgcgt
    16921 tttgtaggcc tgataagacg cggtcagcgt cgcatgaggc aacccgcaca agacaatccc
    16981 ctaaacccca accactttac gcaaaatatc gatatacacc ggcatcaacg ggtggcgaat
    17041 taacaccacc agcgccacca cgccgatggc agaaatcaac ggcgtcagcc acaacaaacg
    17101 gccgcgcgac aaataatgac ttaaacgatc ggttgcttta ccgctgcgcc ataaccgcca
    17161 gcacagccag ccaccaaccc acaaaaatac cgccgtcgcc agcagcaacc atttaaactc
    17221 gccgctctgc attccggctg gaatatcgat cgccgcgccc gccagaatcc caggcaggaa
    17281 gtaaaacggt ggccacagca ggcagccgat aatattcggc gtaatgaatt ttgccaccgg
    17341 cagatccagc attcccgcca ccattggcac cagcggacgc gtcgggccaa caaagcgacc
    17401 gaccagaatg gtgaacatgc tgtgttgatg caacgcatgt tcagttttat cgagtaatgc
    17461 tttgtttttc ttcagaaatg accagcgatg caacggcttt ttaaaacgcc agcccagcca
    17521 gaaggagatc cagtcgccca gcagacaacc gacaatccct gccagccaag cgtgccagaa
    17581 acttaactcg ccgctgccaa tcagcgctcc cagccccgcc atcagcaccg taccgggtag
    17641 aatcaaaccg accagcgcca gcgactccag aaaggccacc aacaccaccg ccatcaatga
    17701 atacacggta gattgggtaa taaagtgttc cagcaatgct tgcataatgt gcctgtcaaa
    17761 tggacgaagc agggattctg caaaccctat gctactccgt caagccgtca attgtctgat
    17821 tcgttaccaa ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
    17881 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
    17941 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
    18001 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct
    18061 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
    18121 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacacgat
    18181 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
    18241 caagcagatt catcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
    18301 tttgcccaaa caggtcgctg aaatacggct ggtgcgcttc atccgggcga aagaaacccg
    18361 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
    18421 aaacccactg gtgataccat tcgcgagcct ccggatgaca accgtagtga tgaatctctc
    18481 ctggcgggaa cagcaaaata tcacccggtc ggcagacaaa ttctcgtccc tgatttttca
    18541 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
    18601 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
    18661 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
    18721 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
    18781 tcttttactg gctcttctcg ctaacccaac cggtaacccc gcttattaaa agcattctgt
    18841 aacaaagcag gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
    18901 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
    18961 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
    19021 acccgttttt ttggatggag tgaaacgatg gcgattgcaa ttggcctcga ttttggcagt
    19081 gattctgtgc gagctttggc ggtggactgc gccagcggtg aagagatcgc caccagcgta
    19141 gagtggtatc cccgttggca aaaagggcaa ttttgtgatg ccccgaataa ccagttccgt
    19201 catcatccgc gtgactacat tgagtcaatg gaagcggcac tgaaaaccgt gcttgcagag
    19261 cttagcgtcg aacagcgcgc agctgtggtc gggattggcg ttgacagtac cggctcgacg
    19321 cccgcaccga ttgatgccga cggtaacgtg ctggcgctgc gcccggagtt tgccgaaaac
    19381 ccgaacgcga tgttcgtatt gtggaaagac cacactgcgg ttgaagaagc ggaagagatt
    19441 acccgtttgt gccacgcgcc gggcaacgtt gactactccc gctatattgg cggtatttat
    19501 tccagcgaat ggttctgggc aaaaatcctg catgtgactc gccaggacag cgccgtggcg
    19561 caatctgccg catcgtggat tgagctgtgc gactgggtgc cagctctgct ttccggtacc
    19621 acccgcccgc aggatattcg tcgcggacgt tgcagcgccg ggcataaatc tctgtggcac
    19681 gaaagctggg gcggcttgcc gccagccagt ttctttgatg agctggaccc gatcctcaat
    19741 cgccatttgc cttccccgct gttcactgac acctggactg ccgatattcc ggtgggcacc
    19801 ttatgcccgg aatgggcgca gcgtctcggc ctgcctgaaa gcgtggtgat ttccggcggc
    19861 gcgtttgact gccatatggg cgcagttggc gcaggcgcac agcctaacgc actggtaaaa
    19921 gttatcggta cttccacctg cgacattctg attgccgaca aacagagcgt tggcgagcgg
    19981 gcagttaaag gtatttgcgg tcaggttgat ggcagcgtgg tgcctggatt tatcggtctg
    20041 gaagcaggcc aatcggcgtt tggtgatatc tacgcctggt ttggtcgcgt actcggctgg
    20101 ccgctggaac agcttgccgc ccagcatccg gaactgaaag cgcaaatcaa cgccagccag
    20161 aaacaactgc ttccggcgct gaccgaagca tgggccaaaa atccgtctct ggatcacctg
    20221 ccggtggtgc tcgactggtt taacggtcgt cgcacgccaa acgctaacca acgcctgaaa
    20281 ggggtgatta ccgatcttaa cctcgctacc gacactccgc tgctgttcgg cggtttgatt
    20341 gctgccaccg cctttggcgc acgcgcaatc atggagtgct ttaccgatca ggggatcgcc
    20401 gtcaataacg tgatggcgct gggcggcatc gcgcggaaaa accaggtcat tatgcaggcc
    20461 tgctgcgacg tgctgaatcg cccgctgcaa attgttgcct ctgaccagtg ctgtgcgctc
    20521 ggtgcggcga tttttgctgc cgtcgccgcg aaagtgcacg cagacatccc atcagcccag
    20581 caaaaaatgg ccagtgcggt agagaaaacc ctgcaaccgc gcagcgaaca ggcacaacgc
    20641 tttgaacagc tttatcgccg ctatcagcaa tgggcgatga gcgccgaaca acactatctt
    20701 ccaacttccg ccccggcaca ggctgcccag gccgttgcga ctctataagg acacgataat
    20761 ggcgattttt gataattatg aagtgtggtt tgtcattggc agccagcatc tgtatggccc
    20821 ggaaaccctg cgtcaggtca cccaacatgc cgagcacgtt gttaatgcgc tgaatacgga
    20881 agcgaaactg ccctacaaac tggtgttgaa accgctgggc accacgccgg atgaaatcac
    20941 cgctatttgc cgcgacgcga attacgacga tcgttgcgct ggtctggtgg tgtggctgca
    21001 caccttctcc ccggccaaaa tgtggatcaa cggcctgacc atgctcaaca aaccgttgct
    21061 gcaattccac acccagttca acgcggcgct gccgtgggac agtatcgata tggactttat
    21121 gaacctgaac cagactgcac atggcggtcg cgagttcggc ttcattggcg cgcgtatgcg
    21181 tcagcaacat gccgtcgtta ccggtcactg gcaggataaa caagcccatg agcgtatcgg
    21241 ctcctggatg cgtcaggcgg tctctaaaca ggatacccgt catctgaaag tctgccgatt
    21301 tggcgataac atgcgtgaag tggcggtcac cgatggcgat aaagttgccg cacagatcaa
    21361 gttcggtttc tccgtcaata cctgggcggt tggctatctg gtgcaggtgg tgaactccat
    21421 cagcgacggc gatgttaacg cgctggtcga tgagtacgaa agctgctaca ccatgacgcc
    21481 tgccacacaa atccacggcg aaaaacgaca gaacgtgctg gaagcggcgc gtattgagct
    21541 ggggatgaag cgtttcctgg aacaaggtgg cttccacgcg ttcaccacca cctttgaaga
    21601 tttgcacggt ctgaaacagc ttccaggtct gcccgtacag cgtctgatgc agcagggtta
    21661 cggctttgcg ggcgaaggcg actggaaaac cgccgccctg cttcgcatca tgaaggtgat
    21721 gtcaaccggt ctgcagggcg gcacctcctt tatggaggac tacacctatc acttcgagaa
    21781 aggtaatgac ctggtgctcg gctcccatat gctggaagtc tgcccgtcga ttgccgtaga
    21841 agagaaaccg atcctcgacg ttcagcatct cggtattggt ggtaaggacg atcctgcccg
    21901 actgatcttc aatacccaaa ccggtccagc gattgtcgcc agcctgattg atctcggcga
    21961 tcgttaccgt ctgctggtta actgtatcga cacggtgaaa acaccgcact ccctgccgaa
    22021 actgccggtg gcgaatgcac tgtggaaagc gcaaccggat ctgccaactg cttccgaagc
    22081 gtggatcctc gctggtggcg cgcaccatac cgtcttcagc catgcgctga acctcaacga
    22141 tatgcgccag ttcgccgaga tgcacgacat tgaaatcacg gtgattgata acgacacccg
    22201 cctgccagcg tttaaagacg cactgcgctg gaacgaagtg tattacggat ttcgtcgcta
    22261 agtagccaca tcaggtatgt aacgcctgat gcgacgctga cgcgtcttat ctggcctaca
    22321 cgctgcgatt ttgtaggccg gataagcaaa gcgcatccgg cattcaacgc ctgatgcgac
    22381 gctggcgcgt cttatcaggc ctacgcgctg cgattttgta gaccggataa gcgaagcgca
    22441 tccggcacga aggagtcaac atgttaaaag atctcaaacg ccaggtatta gaagccaacc
    22501 tggcgctgcc aaaacacaac ctggtcacgc tcacatgggg caacgtcagc gccgttgatc
    22561 gcgagcgcgg cgtctttgtg atcaaacctt ccggcgtcga ttacagcgtc atgaccgctg
    22621 acgatatggt cgtggttagc atcgaaaccg gtgaagtggt tgaaggtacg aaaaagccct
    22681 cctccgacac gccaactcac cggctgctct atcaggcatt cccctccatt ggcggcattg
    22741 tgcatacgca ctcgcgccac gccaccatct gggcgcaggc gggtcagtcg attccagcaa
    22801 ccggcaccac ccacgccgac tatttctacg gcaccattcc ctgcacccgc aaaatgaccg
    22861 acgcagaaat caacggcgaa tatgagtggg aaaccggtaa cgtcatcgta gaaacctttg
    22921 aaaaacaggg tatcgatgca gcgcaaatgc ccggcgttct ggtccattcc cacggcccgt
    22981 ttgcatgggg caaaaatgcc gaagatgcgg tgcataacgc catcgtgctg gaagaggtcg
    23041 cttatatggg gatattctgc cgtcagttag cgccgcagtt accggatatg cagcaaacgc
    23101 tgctggataa acactatctg cgtaagcatg gcacgaaggc atattacggg cagtaatgac
    23161 tgtataaaac cacagccaat caaacgaaac caggctatac tcaagcctgg ttttttgatg
    23221 gattttcagc gtggcgcagg caggttttat cttaacccga cactggcggg acaccccgca
    23281 agggacagaa gtctccttct ggctggcgac ggacaacggg ccgttgcagg ttacgcttgc
    23341 accgcaagag tccgtggcgt ttattcacgc cgatcaggtt ccccgcgctc agcatatttt
    23401 gcagggtgaa caaggctttc gtctgacacc gctggcgtta aaggattttc accgccagcc
    23461 ggtgtatggc ctttactgtc gcgcccatcg ccaattgatg aattacgaaa agcgtctgcg
    23521 tgaaggtggc gttaccgtct acgaggccga tgtgcgcccg ccagaacgct atctgatgga
    23581 gcggtttatc acctcaccgg tgtgggtcga gggtgatatg cacaatggca ctatcgttaa
    23641 tgcccgtctg aaaccgcatc ccgactatcg tccgccgctc aagtgggttt ctatagatat
    23701 tgaaaccacc cgccacggtg agctgtactg catcggcctg gaaggctgcg ggcagcgcat
    23761 cgtttatatg ctggggccgg agaatggcga cgcctcctcg cttgatttcg aactggaata
    23821 cgtcgccagc cgcccgctgc tgctggaaaa actcaacgcc tggtttgcaa accacgatcc
    23881 cgatgtgatc atcggctgga acgtggtgca gttcgatctg cgaatgctgc aaaaacatgc
    23941 cgagcgttac cgtcttccgc tgcgtcttgg gcgcgataat agcgagctgg agtggcgcga
    24001 gcacggcttt aaaaacggcg tcttttttgc ccaggctaaa ggtcggctaa ttatcgacgg
    24061 tatcgaggcg ctgaaatccg cgttctggaa tttctcttca ttctcgctgg aaactgtcgc
    24121 tcaggagcta ttaggcgaag gaaaatctat cgataacccg tgggatcgaa tggacgaaat
    24181 tgaccgccgt ttcgccgaag ataaacctgc gctggcaact tataacctga aagattgcga
    24241 gctggtgacg cagatcttcc acaaaactga aatcatgcca tttttactcg aacgagcaac
    24301 ggtgaacggc ctgccggtgg atcggcacgg cggttcggtg gcagcgtttg gtcatctcta
    24361 ttttccgcga atgcatcgcg ctggttatgt cgcgcctaat ctcggcgaag tgccgccgca
    24421 cgccagccct ggcggctacg tgatggattc acggccaggg ctttatgatt cagtgctggt
    24481 gctggactat aaaagcctgt acccgtcgat catccgcacc tttctgattg atcccgtcgg
    24541 gctggttgaa ggcatggcgc aacctgatcc agagcacagt accgaaggtt ttctcgatgc
    24601 ctggttctca cgggagaaac attgcctgcc ggagattgtg actaacatct ggcacgggcg
    24661 cgataaagcc aaacgtcagg gtaataagcc gctttcgcag gcgctgaaga tcattatgaa
    24721 tgccttttat ggcgtgctcg gcaccaccgc ctgccgcttc ttcgatccgc gcctggcatc
    24781 gtcgatcacc atgcgtggtc atcagatcat gcggcagacc aaagcattga ttgaagcgca
    24841 gggctacgac attatctacg gagataccga ctcgactttt gtctggctaa aaggtgcaca
    24901 ttcggaagaa gaggcggcga aaatcggtcg tgcactggtg cagcacgtta acgcctggtg
    24961 ggcggaaacg ctgcaaaaac aacggctgac cagcgcatta gaactggagt atgaaactca
    25021 tttctgccgt tttcttatac caaccattcg cggagctgat accggcagta aaaagcgcta
    25081 cgccgggctg attcaggagg gcgacaagca gcggatggtg tttaaagggc tggaaaccgt
    25141 gcgcaccgac tggacgccgc tggcccagca gtttcagcag gagctgtacc tgcgtatctt
    25201 ccgcaacgag ccatatcagg agtacgtccg ccaaaccatc gacaaactga tggcgggtga
    25261 actggatgcg cggctggttt accgtaaacg ccttcgccgt ccgctgagcg aatatcagcg
    25321 taatgttccg cctcatgtac gcgccgctcg ccttgccgat gaagaaaatc aaaagcgcgg
    25381 tcgccccttg caatatcaga atcgcggcac cattaagtac gtatggacca ccaacggccc
    25441 ggagccgctg gactaccaac gttcaccact ggattacgaa cactatctga cccgccagct
    25501 acaacccgtg gcggagggaa tactcccttt tattgaggat aattttgcta cacttatgac
    25561 cgggcaactt gggctatttt gagcaaaaaa aagagttcgc cagataccat tttgatgcgt
    25621 gacgaatgct ttgccatcca gtaccatagc gccctttcca ttcctggacc tgaataacac
    25681 cactacctca taagcacggt agcgggtggt tattgcctgc aattaaagat atagagccga
    25741 acacatatgc cttttacact tggtcaacgc tggatcagcg atacagaaag cgaattggga
    25801 cttggaaccg ttgtcgcggt ggatgcgcga actgtcactt tacttttccc atctactggt
    25861 gaaaaccgtc tgtacgcacg cagtgattcc cccgtgaccc gcgtgatgtt caaccctggt
    25921 gataccatta ccagccatga cggctggcag atgcaagtcg aagaagtaaa agaagaaaat
    25981 ggcttgctga cctatatcgg tactcgcctg gatactgaag agtccggcgt agccctgcgt
    26041 gaagttttcc ttgatagcaa actggtgttc agcaaaccgc aggaccgtct gtttgccggg
    26101 cagattgacc gtatggaccg ctttgcgctg cgttatcgcg cgcgtaagta ttccagcgaa
    26161 cagttccgta tgccgtacag cggcctgcgc ggtcagcgta ccagcctgat cccgcatcag
    26221 ctcaacatcg ctcatgatgt tggtcgccgc cacgcgccgc gcgtcctgct ggctgacgaa
    26281 gtgggtttag ggaaaaccat tgaagccggg atgatcctgc atcagcaact gctctctggc
    26341 gctgctgaac gtgtgctgat tatcgtcccg gaaaccttac agcatcagtg gctggtagaa
    26401 atgctgcgcc gtttcaacct gcgctttgct ctgtttgatg atgagcgtta tgccgaagct
    26461 cagcacgatg cttacaaccc gtttgacacc gaacagctgg tgatttgctc gctggatttt
    26521 gcccgtcgta gcaaacagcg cttggaacat ctctgtgaag ccgaatggga cctgctggtg
    26581 gtcgatgaag cgcatcacct ggtgtggagc gaagatgcgc caagccgtga atatcaggcc
    26641 attgaacaac tggcagagca cgtgccgggc gttctgctgc tgaccgcgac cccggaacag
    26701 ctggggatgg aaagccactt cgcccgtctg cgtctgctgg acccgaaccg tttccacgat
    26761 tttgcgcagt tcgttgaaga gcagaaaaat tatcgtccgg ttgcggacgc cgttgccatg
    26821 ctgctggcag gtaacaaact gagcaatgac gaactgaaca tgctcggcga gatgatcggc
    26881 gagcaggata tcgagccgct gttgcaggca gcaaacagcg acagcgaaga tgcccagagc
    26941 gcccgtcagg agctggtttc gatgctgatg gatcgccacg gcaccagccg cgtgctgttc
    27001 cgtaacacgc gtaacggtgt gaaaggattc ccgaaacgcg agctgcacac cattaagctg
    27061 ccgctaccga cgcagtatca gacggctatt aaagtctccg gcattatggg cgcacgtaaa
    27121 agtgcggaag atcgtgctcg cgatatgctc tacccggagc gtatttatca ggaatttgaa
    27181 ggtgataacg ccacctggtg gaacttcgat ccgcgcgttg agtggctgat gggctacctg
    27241 accagccatc gctctcagaa agtgctggtg atctgcgcca aagctgccac tgcgctgcaa
    27301 ctggagcagg tactgcgcga acgtgaaggt attcgcgctg cggtgttcca cgaaggtatg
    27361 tcgattatcg aacgtgaccg cgctgccgcc tggtttgccg aagaagacac cggcgcacag
    27421 gtactgctgt gctcagaaat cggttctgaa ggacgtaact ttcagttcgc cagtcacatg
    27481 gtgatgtttg acctgccatt caacccggat ctactggagc agcgtattgg tcgtctggat
    27541 cgtatcggcc aggcgcacga tattcagatc catgtgcctt atctggagaa aaccgctcag
    27601 tcggtgctgg tgcgctggta tcacgaaggt ctggatgcat ttgagcacac ctgcccgacc
    27661 ggacgcacta tttacgatag cgtatacaac gatctgatta actatctggc ttcaccggat
    27721 caaaccgaag gctttgacga tctgatcaaa agctgccgcg agcaacatga agcgctgaaa
    27781 gcacagctgg aacagggtcg tgaccgcctg ctggaaatcc actccaacgg tggcgaaaaa
    27841 gcccaggcac tggcagaaag cattgaagag caggatgacg ataccaacct gatcgccttc
    27901 gccatgaacc tgttcgatat tatcggtatc aatcaggacg atcgcggcga caacatgatc
    27961 gtgctgacgc cgtccgatca tatgctggtg ccggacttcc ctggcctgtc ggaagatggc
    28021 atcaccatca cctttgatcg tgaagtggcg ctggcgcgtg aagatgcaca gtttattacc
    28081 tgggagcatc cgctgatccg caacggtctg gatctgatcc tttctggcga taccggtagc
    28141 agcacgattt cactgttaaa aaacaaagcg ttgccggtag gtacgctgtt ggtggaactg
    28201 atttatgtgg ttgaagccca ggctccgaag cagttgcagc tcaaccgctt cctgccaccg
    28261 acgccggtac gtatgctgct ggataaaaac ggcaacaacc tggcggcgca ggtagagttt
    28321 gaaaccttta accgccagct taacgcggtt aaccgtcaca ccggcagcaa actggttaac
    28381 gccgtgcagc aggatgttca cgctatcctt caactgggtg aagcgcagat cgagaaatct
    28441 gcccgtgcat tgattgatgc agcgcgtaac gaagccgacg aaaaactgtc tgccgagctg
    28501 tctcgtctgg aagctctgcg tgcagtgaac ccgaacattc gtgacgacga actgaccgcc
    28561 attgagagca accgtcagca ggtaatggaa agcctggatc aggcaggttg gcgtctggat
    28621 gccctgcgtt tgatcgttgt aacgcatcag taacggagcc gaaaatgggg atggaaaact
    28681 acaatccacc gcaggaaccc tggttggtta tcctgtatca ggatgaccat attatggtgg
    28741 tcaacaagcc gagcggtttg ttgtcagtgc cgggtcgtct ggaagagcac aaagacagcg
    28801 tgatgacgcg cattcagcgt gattatccgc aggcagaatc ggtgcatcgt ctggatatgg
    28861 ctaccagcgg cgtgattgta gtggcgctga ccaaagccgc ggagcgggag ttaaaacgcc
    28921 agttccgcga gcgcgagccg aaaaagcagt atgtggcccg cgtctggggg catccatccc
    28981 ccgcagaagg tctggtggat ctgccgctga tttgcgactg gccaaaccgc ccgaaacaga
    29041 aagtctgtta cgaaacgggt aaacctgcgc agacggaata tgaagtggtg gagtatgcgg
    29101 cggataacac ggcaagagtg gtgttaaaac cgattaccgg gcgttcgcat caattgcgtg
    29161 tgcatatgct ggcgctgggg catccgattc tcggcgatcg tttttatgca tcaccagaag
    29221 cgagagcgat ggcaccgcgt ttgttgctac atgcagagat gttgacgatt acccatccgg
    29281 cgtatggcaa tagtatgacg tttaaagcgc cagcggattt ttaatcgtca cgctggcgca
    29341 gttcggtagg cggataaggc gttcacgccg catccggcaa ttgtgcgccg attgcctgat
    29401 gcgacgcggg ccgcgtctta tcaggcctac agtttaaaac ttatttaaac cctttctgct
    29461 gctttatcag ctcatatgcc tgctgaattt cctgcgcttt ctgcttcgcc atctccatca
    29521 tctcaggcgg caaacctttc gccaccagct tatcgggatg gtgttcactc atcagcttac
    29581 ggtaggcacg tttgatggtg gtcgcatcat ccgtcggctt cacgcccagc acattacagg
    29641 catcttccag cgtcgggcca cgctgcgctt gctgccagtt accaccgcca gattgctgct
    29701 gataaccgcc gccaaactgt gcaccgccct gcatcatgcg caaaaactgg tcaaactggg
    29761 cgcgggagat ccctaattct tcagcaatga catacagcac cgcccgttca ttcgggtgca
    29821 gtgaaccatc agcaaacgcc gcctgaatct ggatctccag aaacatacga attaagtcaa
    29881 aacgaccaaa gcagacactg cgaaactggc gcatcttttc gcgcagcggg taattatctg
    29941 attttcccac ccggaacgcg ttttgcgccg cagtacggga agcgccatga aggttcattc
    30001 ggtccatcaa ctggctggcg atatgaatat cagcctccgt gacgcgacct ttggatttgg
    30061 ttaaatgccc catcacttca aaagtggtgg caaaaaacag cgcctgacgc tcacgctggt
    30121 tggcgaacca cgccatttta cggctacggg ctttatcaaa catatggcca attaacaggc
    30181 ctaacactac gccccaaaag ccgccgccca tcagtaaggc cacggccacg ccaatgattt
    30241 ttccccaata ctgcatatat tccccaaatc gacacacgga tatcagggct atctcccaca
    30301 atataaaggt gcttttaccg ttttccggct gcggtcaatt gtgggacatc gcctataatt
    30361 tgcattatca tacctgtcat tcaatgccgt gcctaacacc actgacgcta ttcggacagg
    30421 attaacacta gcgtagagat gacgagtacg ttagtctctg accgtttgtc acgcgcaacg
    30481 ttaccgatga tggaacaata aaatcaacgt atgaaaaaac gtatccccac tctcctggcc
    30541 accatgattg ccaccgccct ttatagtcaa cagggactgg cagccgacct cgcctcacag
    30601 tgcatgttgg gcgtgccaag ctatgaccgt cctctggtac agggcgatac caatgactta
    30661 cccgtgacta tcaatgctga ccacgcgaaa ggggactacc cggatgacgc cgtgtttact
    30721 ggcagcgtgg atatcatgca gggtaacagc cgtctgcagg ccgacgaagt gcagctccat
    30781 caaaaagagg caccaggaca accggagccg gtacgtaccg ttgatgcgct cggtaatgtc
    30841 cattacgacg ataaccaggt gatcctcaaa gggccgaaag gctgggcgaa tctgaacacc
    30901 aaagatacca acgtctggga aggtgattac cagatggtgg gtcgccaggg tcgcggtaaa
    30961 gcggacctga tgaaacaacg tggcgaaaac cgctatacca ttctggataa cggtagcttt
    31021 acctcctgtc tgccgggttc tgacacctgg agcgtggtag gtagcgaaat tattcatgac
    31081 cgcgaagaac aagttgctga gatctggaac gcccgcttta aggtgggtcc ggtaccgatc
    31141 ttttatagcc cctatttgca gttgccggtg ggtgacaaac gtcgctctgg tttcttgatc
    31201 ccgaacgcca agtacaccac caccaactac tttgagttct acctgccata ttactggaac
    31261 atcgcgccaa atatggatgc caccatcacg ccgcattata tgcatcgtcg tggcaacatc
    31321 atgtgggaga acgaattccg ctacctctcc caggcgggcg ctggcttgat ggaactggac
    31381 tatctgcctt cagataaagt ctatgaagat gaacacccga acgatgacag ttcacgtcgt
    31441 tggttattct actggaacca ctccggggtc atggatcagg tgtggcgttt caacgtcgac
    31501 tacaccaagg tcagcgatcc tagctacttc aatgatttcg ataacaagta cggttccagt
    31561 actgacggct acgcaacgca aaaattcagc gttggctatg cggtgcaaaa cttcaatgcc
    31621 accgtttcaa ccaagcagtt ccaggttttc agcgaacaga acaccagtag ctactcggca
    31681 gagccgcagt tagacgttaa ttactaccag aatgatgttg gtccgtttga tacgcgtatt
    31741 tacggccagg cagtgcactt tgttaacacc agagacgaca tgcctgaagc aacccgtgtt
    31801 cacctggaac cgaccatcaa tttgccgctc tctaataact ggggcagcat caataccgaa
    31861 gcgaagtttc tggcaaccca ttatcagcaa accaatcttg actggtataa ctccagaaac
    31921 acgaccaagc tggacgaatc cgttaaccgc gtaatgccgc aattcaaagt tgacggcaaa
    31981 atggtctttg aacgcgatat ggaaatgctg gctccgggtt atacccaaac gctggaaccg
    32041 cgcgcgcagt atttgtacgt gccgtatcgc gatcagagcg acatctataa ctacgactcg
    32101 tctctgctgc aatctgacta ctctggcctg ttccgggacc ggacttacgg cggtcttgac
    32161 cgtattgcct ccgctaacca agtgacgacc ggtgtcacat ctcgcatata tgatgatgct
    32221 gccgttgaac gttttaatat ttccgttggt caaatctact atttcacgga gtctcgcact
    32281 ggcgatgaca acataacatg ggagaatgac gacaaaacgg gctcactggt gtgggcaggc
    32341 gacacctact ggcgtatctc cgagcgttgg ggattgcgtg gcgggattca gtacgataca
    32401 cgtctggata acgtagcgac cagtaactcc agcattgaat accgtcggga tgaagaccgt
    32461 ctggtacagc tgaattacca ttacgccagc ccggaatata ttcaggctac gctgcctaag
    32521 tactattcca ctgctgagca atataagaat ggtatttcgc aggtaggtgc tgtcgccagc
    32581 aggccaattg ccgatcgttg gtccattgtt ggggcctact actacgacac caatgcgaac
    32641 aagcaagccg actctatgtt aggtgtgcaa tacagctcct gctgctatgc aattcgcgtc
    32701 ggttacgagc ggaagctgaa cggttgggat aacgataaac aacatgcggt atatgacaac
    32761 gcaatcggct ttaacatcga acttcgcggc ctgagctcca actacggtct gggtacgcaa
    32821 gagatgctgc gttcgaacat tctgccgtat caaaacactt tgtgatttgt tgatttacca
    32881 cgtaatccgc agtgcggtta attgaaatgg aaaaagtatg aagaactgga aaacgctgct
    32941 tctcggtatc gccatgatcg cgaataccag tttcgctgcc ccccaggtag tcgataaagt
    33001 cgcagccgtc gtcaataacg gcgtcgtgct ggaaagcgac gttgatggat taatgcagtc
    33061 ggtaaaactg aacgctgcgc aggcaaggca gcagcttcct gatgacgcga cgctgcgcca
    33121 ccaaatcatg gaacgtttga tcatggatca aatcatcctg cagatggggc agaaaatggg
    33181 agtgaaaatc tccgatgagc agctggatca ggcgattgct aacatcgcga aacagaacaa
    33241 catgacgctg gatcagatgc gcagccgtct ggcttacgat ggtctgaact acaacactta
    33301 tcgtaaccag atccgcaaag agatgattat ctctgaagtg cgtaacaacg aggtgcgtcg
    33361 tcgcatcacc atcctgccac aggaagtcga atccctggcg cagcaggtgg gtaaccaaaa
    33421 cgacgccagc actgagctga acctgagcca catcctgatc ccgctgccgg aaaacccgac
    33481 ctctgatcag gtgaacgaag cggaaagcca ggcgcgcgcc attgtcgatc aggcgcgtaa
    33541 cggcgctgat ttcggtaagc tggcgattgc tcattctgcc gaccagcagg cgctgaacgg
    33601 cggccagatg gggtggggcc gtattcagga gttgccgggg atcttcgccc aggcattaag
    33661 caccgcgaag aaaggcgaca ttgttggccc gattcgttcc ggcgttggct tccatattct
    33721 gaaagttaac gacctgcgcg gcgaaagcaa aaatatctcg gtgaccgaag ttcatgctcg
    33781 ccatattctg ctgaaaccgt cgccgatcat gactgacgaa caggcccgcg tgaaactgga
    33841 gcaaattgct gctgatatca agagtggtaa aacgactttt gctgccgcag cgaaagagtt
    33901 ctcgcaggat ccaggttctg ctaaccaggg cggtgatctc ggctgggcta caccagatat
    33961 tttcgatcca gccttccgtg acgccctgac ccgcctgaac aaaggtcaaa tgagtgcacc
    34021 ggttcactct tcattcggct ggcatttaat cgaactgctg gatacccgta atgtcgataa
    34081 aaccgacgct gcgcagaaag atcgtgcata ccgcatgctg atgaaccgta agttctcgga
    34141 agaagcagca agctggatgc aggaacaacg tgccagcgcc tacgttaaaa tcctgagcaa
    34201 ctaatggtta aaacccaacg tgttgtgatc actcccggcg agcccgccgg gattggcccg
    34261 gacttagttg tccagcttgc acagcgtgag tggccggtcg aactggttgt ttgtgccgat
    34321 gccactctcc ttaccgaccg ggcagcgatg ctcggtttgc cgctcaccct ccgcccttat
    34381 tcccccaact cccctgcaca accgcaaact acgggcacat taacgctact tcctgtcgcg
    34441 ctacgtgaat ctgtcactgc ggggcagtta gcgattgaaa atgggcatta cgtggtggag
    34501 acgctggcgc gagcgtgtga tggctgtctg aacggtgaat ttgctgcgct gatcacaggc
    34561 cccgtgcata aaggcgtcat taacgacgca agcattccgt ttaccggtca taccgagttt
    34621 ttcgaagagc gttcgcaggc gaaaaaagtg gtgatgatgc tggcgaccga agaacttcgc
    34681 gtggcgctgg caacgacgca tttaccgctg cgcgatatcg cagatgctat cacccctgca
    34741 cttttgcacg aagtgattgc tattttgcat cacgatttgc gtacaaaatt tggtattgcc
    34801 gaaccgcgca ttctggtctg cgggctgaat ccgcacgcgg gcgaaggcgg tcatatgggt
    34861 acggaagaga tagacaccat tattccggtg ctcgacgagc tgcgggcgca ggggatgaaa
    34921 ctcaacgggc cgctgcctgc cgataccctg tttcagccga aatatctcga taacgccgac
    34981 gccgtgctgg cgatgtacca cgatcagggt cttcccgtgc taaaatacca gggcttcggg
    35041 cgcggtgtga acattacgct gggcctgccc tttattcgca catcagtgga ccacggcacc
    35101 gcgcttgaac tggcgggacg tggcgaagcc gatgtcggca gttttattac ggcgcttaat
    35161 ctcaccatca aaatgattgt taacacccaa tgaataatcg agtccaccag ggccacttag
    35221 cccgtaaacg cttcgggcaa aactttctca acgatcagtt cgtgatcgac agcattgttt
    35281 ctgccattaa cccgcaaaag ggccaggcaa tggtcgaaat cggccccggt ctggcggcat
    35341 tgaccgagcc ggtcggcgaa cgtctggacc agctgacggt catcgaactt gaccgcgatc
    35401 tggcggcacg tctgcaaacg catccattct taggcccgaa actgacgatt tatcagcagg
    35461 atgcgatgac ctttaacttt ggcgaactgg ccgagaaaat gggtcagccg ctgcgtgttt
    35521 tcggcaacct gccttataac atctccacgc cgttgatgtt ccatctgttt agctatactg
    35581 atgccattgc cgacatgcac tttatgttgc aaaaagaggt ggtgaatcgt ctggttgcag
    35641 gaccgaacag caaagcgtat ggtcgattaa gcgtcatggc gcaatactat tgcaacgtga
    35701 tcccggtact ggaagttccg ccgtcagcct ttacaccacc acccaaagtg gattccgccg
    35761 tcgtgcgcct ggtccctcat gcaacgatgc ctcacccggt taaagatatt cgcgtgttga
    35821 gccgcatcac caccgaagcc tttaaccagc gtcgtaaaac cattcgtaac agcctcggca
    35881 acctgtttag cgtcgaggtg ttaaccggaa tggggatcga cccggcgatg cgagcggaaa
    35941 atatctctgt cgcgcaatat tgccagatgg cgaactatct ggcggagaac gcgcctttgc
    36001 aggagagtta acgatgatca attcgccccg agtgtgtatt caggttcaaa gcgtctacat
    36061 tgaggcacaa tcttcacctg ataatgaacg ttacgttttt gcttataccg taaccatacg
    36121 caatctgggg cgagcgccag tgcagttgtt ggggcgttac tggctgatca ccaatggcaa
    36181 tggccgtgaa accgaagtcc agggcgaagg agtggttggc gtccagccac ttatcgcgcc
    36241 tggcgaagag taccagtaca ccagcggtgc aatcattgaa accccgctgg gcaccatgca
    36301 gggtcactac gaaatgatcg atgaaaatgg cgtccctttc agcatcgaca ttcccgtatt
    36361 ccgactcgcc gttcccacac tcattcatta aaagaatatg gcgacatacc ttattggcga
    36421 cgttcatggt tgttacgatg aactgatcgc attgctgcat aaagtagaat ttacccccgg
    36481 gaaagatacc ctctggctga cgggcgatct ggtcgcgcgc ggcccgggtt cgctggatgt
    36541 tctgcgctat gtgaaatcct taggcgacag cgtacgtctg gtgctgggca atcacgatct
    36601 gcatctgctg gcggtatttg ccgggatcag ccgcaataaa ccgaaagatc gcctgacacc
    36661 gctgctggaa gcgccggatg ccgacgagct gcttaactgg ctgcgtcgcc agcctctgct
    36721 gcaaatcgac gaagagaaaa agctggtgat ggcccacgca gggatcacgc cgcagtggga
    36781 tctgcagacc gccaaagagt gcgcgcgcga tgtagaagcg gtgctgtcga gtgactccta
    36841 tcccttcttt cttgacgcta tgtacggcga tatgccaaat aactggtcac cggaattgcg
    36901 ggggctggga agactgcgtt ttatcaccaa cgcctttacc cgtatgcgtt tttgcttccc
    36961 gaacggtcaa ctggatatgt acagcaaaga atcgccggaa gaagcccctg ccccactgaa
    37021 accgtggttt gcgattcctg gccctgtcgc tgaagaatac agcatcgcct ttggtcactg
    37081 ggcatcgctg gaaggcaaag gtacgccgga aggtatttac gcgctggata ccggctgctg
    37141 ctggggtggg tcattaacct gcctgcgctg ggaagataaa cagtattttg tccagccgtc
    37201 gaaccggcat aaggatttgg gcgaagcggc ggcgtcttaa acacagcctg atacaggaag
    37261 gccggataag acgcgaccgg cgtcgcatcc ggcactagcc gtaaattcta tacaaactta
    37321 ccgccgctcc agaatctcaa agcaatagct gtgagagttc tgcgcatcgg cgtcgtggaa
    37381 ttcgctgaat accgattccc agtcatccgg ctcgtaatcc gggaaatggg tgtcgccttc
    37441 cacttctgcg tcgatatgcg tcagatacag tttttgcgct tttggcagga actgctcata
    37501 aacgcgaccg ccgccaatca ccatgatttc tggtacgtca ccacacgccg caatggcttc
    37561 atccaccgat ttcacccacg ttacgcgatc gtccgtaccc ggttgactgc tgaggataat
    37621 atttttgcgt cctggcaacg gacgaccgat tgattcccaa gtatggcggc ccataatcac
    37681 gggtttattt aaggtgttgc gtttaaacca ggcgagatcg gcaggcaggt tccacggcat
    37741 ggcgttttcc atgccgataa cgcgatctac cgctaacgcc gcaatcagac tgatcattga
    37801 gatttcccga taaaaaaatt gtcgccacta tacgtaaagc gtaaaccgtc gtcgactggt
    37861 gcgaggatga tgttgaggaa aattttatat tctgctggcg agtccacgct ctccccctgg
    37921 actcgccgca ttacaatgaa acaaaaacaa acagttagct gtaaagtgtg atttacgtca
    37981 ctctttatta agatgagggt ttcgtttccg gttcatccgc catgttgccg gtatgtttac
    38041 cttcttctgt tccctgccag ccatgtcgtt gaattaatga cagatgttcg cggtcctcgg
    38101 tgatgatctc acttaacatc gcgctggtgc gtttatagac cgccgcgcgg gctttggtgt
    38161 cattctcaac cattgccatc tcttccacca tctgaatatt aaagcggcgg aacacatcgg
    38221 cacgttctcg cgcttcatac ggccccagac ctaaactttc cagcgccaga cgcccggttt
    38281 tcagcgcacc ttcaaaggtt tcacgctccg gcttttcaac gcctgcctga cgcaaacgaa
    38341 tgtagtgatc gacatcgcgg gcgcgggcaa taatctgcaa atgcgggaaa tgttctttca
    38401 ccatctccgt cagttgcagg ttggtttgcg gatcgtcaat agcgttaatc agcacttccg
    38461 ctttcgccgc tccggcagat tccagtaaat ccatccgcgt ggcatcgcca taaaacactt
    38521 tcataccaaa tttacgcagc gtttcgatat ggtccggatc gtgatcgagc accaccattt
    38581 tcaccccgct ggagagcagt aaacgtccgg taatctgtcc aaaacgaccg aatccggcga
    38641 taatcacgcg cggctgttct tcatcgatct catcggcttc acgcgcctcc tccgtagaag
    38701 attgctcaag gcgattgagg atcaccagca gaatcggcgt tgctgccatc gacagcgcca
    38761 ccgccagggt cagcgatttc gcccactccg gctccagcac attcgccatc tgcgccgcac
    38821 caaataccac aaaggcaaac tcactgccct gccctaacaa caccgcaaac cagcgacgct
    38881 gtttatttgg cacctgcaac ggtcgggcaa tcagccacag catggcgatt ttgatgatga
    38941 ggaaaccgag cagcaaaatg acaatgcgca atgggttttc aatcagcgtg ccaaagtcta
    39001 tcgacatgcc aacaccgatg aaaaacagcc ccaacagcaa acctttaaac ggttcgatat
    39061 cgctttccaa cgcatgacgg tattccgagc ttgccagcag tacgcccgcc agaaacgcgc
    39121 ccatcgccat cgacaagccg acctcttcca gcagcaaacc aaagccaaac acaaggaata
    39181 acgccacggc actaaacact tcccgcaagc cagagcgggc aacaaaacgc agcgccggac
    39241 gcgtaacata gcgccccagc aataccacca gtaccagcgc acccgccact tttaacgccg
    39301 agagagcaaa tgcgcccatc gtcgtcgagg cactgctcgc cgccagtagc gggatcatcg
    39361 ccaccagcgg gattgccgcg atatcctgga acagcagcac tgcaaaggca ctgcggccca
    39421 tttgcgtcac catcagattg cgttcattca tcgcctgcat ggcaatcgcc gttgatgaga
    39481 gcgccagcgt cataccgatc aattccgcga cctgccagcg cagcccaagt aacatacaga
    39541 acagccccag caggccaccg caaatcacca tctgcaatgc gccaccgccg aacactgccg
    39601 cacgcagctt ccacagcctt tgtggatcga gttcgaggcc gataataaac agcatcagca
    39661 ccaccccaat ctcggcaaag tgcagaatag attcggcatc ggtcaccagt cgcagccccc
    39721 acgggccaat aatgcagccg gcgatcaggt agccaagtcc cgatcccaga ccaagacgta
    39781 ccgcaatggg tacaatcagc gccgccgatc cgaggtagat cagcgcctga accagcgtat
    39841 gactatccat gatgggcctc ctgccattcc agcagacgtt gcttatagtg gcgcgcctgc
    39901 ccttcgaggg tttcgtcgtc acaaataaag gtgcagtgca tggcaaacgg tggcagccag
    39961 ttcagcccgc agtagattgc cgtcgcctgt agcggctgcg acagcacatc aaagcccgga
    40021 tgcgcaccaa tttcaaaatg gctttccccg ccgccggtcg tcaccgccca cagcaaatgt
    40081 ttgccatgca gcgccgtgcc gccgtgaccg taagcccagc cgtgcgacaa aactttatcg
    40141 atccaaagtt taagaagcgg aggaatgctg taccactgca tcgggtgctg ccagacgatc
    40201 agatcggcgc gagagagcgc ctcctgctcg gcggcaatat cgatattgaa gtcaggatag
    40261 agttgataaa gagagcgaat ttcgacgcct tccagcgtcc ttgcctgttc aagcatccgt
    40321 ttattcgcat gggaatgatg cggatacgga tgcgcataaa tgatgaggat catagttatt
    40381 agcccgcgtg tttacgcctt tgttttccca caaagtgtag tcatatattc acgcggctta
    40441 tagtcaataa tattgattca gaaaagagag aaatctgaac aaataatccc attctctgcc
    40501 cgcggatcgt catattgctg gtctgcgcca gtgacattcc tcgagtctcc ggggcaaacg
    40561 ctacggaaat caacaagcca aacagcgaga tacccgcccc cattagcatc gtgttactga
    40621 taccgtaatt attgataaat atcggcagtg cccaggtcga aacaatggtg ccaatacgac
    40681 ttaaggacat aatcacgccc acggcagagg cgcggatatc cgtcgggaag agttcattag
    40741 gatagagcca ctgcaaatta cccggcccgc cagagaaaaa ggcatacacc gcaaaggcca
    40801 tcactaccag ccagatcccc atatccggga ttagccccaa aaccgccaac gccagcgtca
    40861 tcatggcaaa gctgccaatc aacaatggac gccgtccggc agtgtttagc cacagcatcg
    40921 gcggaataca gccgagcata aagaacaggc taatcaccac attccctagt gccgcgtttt
    40981 tgccaacccc caatcccaac gaaccaacga tttgcgggcc aaaggtgtaa atggcgaaca
    41041 ttgggatcac ctggcaggtc cagatggcgg caacaaacag aacaaaagga aaatggcggc
    41101 gattaaacag atcgcgaaaa cgggtttgct gcggctgctc ttcatcgaaa gccaccggtt
    41161 cgccaaacag ttttatcatc atctcttcgc actcttttac tcgccctttg cgtaataacc
    41221 agcggggaga ttctggcagt tcgaatcgac caatcaaaat caacaaacaa gggatcgccg
    41281 cgctacccag catccagcgc caaccacctt ccacatcata aagccagtag ccgaccagat
    41341 cggcgcaggt cgcgccgaca taccacatcg cggcaataaa gctgatggaa aacgcccgct
    41401 gacgggtact ggagaactcg gtgatcatcg aagtggcgat gggataatct gcaccgatga
    41461 caatgccgat gagtacccgc atcaccaaca gttcgacggg ggatgaaaca cacatcgtcg
    41521 ccaccgatat cacgccgatg gcgatgatat caatgaggaa cattttgcgc cgtccgactt
    41581 tatcggagat atagccaaac agcgatgtgc caacgaacag cccggcgagc gttcccgcgc
    41641 ccagcaagcc aatccagtca gcgtccagtt tcagcgccgg agtcagttgc tccagcgcca
    41701 cgccaatcat taccagtaca taaccatcca gaaacggacc accgcttccc cacagcaaaa
    41761 tgcggcggtg aatagaggag aatttgagat cgtcaaagtt tctggacggt tgcatgggtt
    41821 attcctgttt ttttgcaatg agagtcagta cggcgttacg tatcaaacca acatcagccg
    41881 taacggaact ccacaccaaa ggtgccgcgc gggtattccc actgttccag cgccgacccc
    41941 agccccagaa tgcgacaggt gccgcactcc agacatccgg cgtaatcgaa gcgcacactg
    42001 ccgtcatcct gcttcttgta cagacctgcg gggcacgctt tcaccagcag ctccagcacc
    42061 tgtttatcag catcggcctt cacaacaatg tgcggatgct cttcatcgac attgaattta
    42121 ttgacgccca gtttgacgtc cacattgacg ggagaagtca taaaacggtc actcctttca
    42181 tgccatcctt gatcagattg atgaagccca ctttcttgcc gtggcggagg attttcttgc
    42241 gcatcagttc cggcgcactg ccgtcaatgg tgaacaggtc acgcgccacg cccaccgcca
    42301 gttccgggtt gccgctaaac atgcgtgggt tatcaaggaa cgccggtagt ttctggtaca
    42361 tgcgcatatc gcgcagcggg ccactctcca gatgctgacg atattccgcg agtttttgct
    42421 tactgaaatc gtcgcttttc atcgctgaaa gcacggtttt tgctgcagct tccccggcgg
    42481 caatcgccag atccataccg cgaatggtaa aaccgaggtt catacacatt ccggcggcat
    42541 caccggcaat caatacgccg tcaccgacca gctccggcag catgttgatg cctgcttccg
    42601 gcactacgtg agcggaatat tccaccagct tgccgcccgc gatcagcggt gcaacggccg
    42661 gatgctgttt gaaatcttcc agcatttgcg gcaccgattt tttcgcgtca tgcagatgat
    42721 gcagaccaca aaccagcccc agcgacaggg tattttcatt ggtataaagg aagccgccgc
    42781 ccatcaggcc atcggtgggt gatcccgcaa acagacaagc cgcgccctga ttaccctgca
    42841 actgaaaacg gtcttcgatt accgacttcg gtaactcgat cagttccttc acgccaaccg
    42901 ccacatccgt cggtttaacg cgttttgcca tccccaactt ttcggcaagg atggagttca
    42961 ccccatcagc aaggagcacc gttttcgctt caatcacatc accatcggct tctacaccga
    43021 cgactttgcc atcgcgctgt acgaggttat cgacgcggat cccggtaatt aactgcgcgc
    43081 ccgcttcttc ggcctgctcc atcaaccagg catcaaattt actgcgcaaa acggagtaag
    43141 aacgctggga tggcgaggtt tcgtcaccgt tgcagtagtc catagtcatc gctgactttt
    43201 ccgtcataaa cgcgagtttt tcatgggtga tcaggcgttc tacgggggcg gaatcggcga
    43261 aacctggaat aatgtgttcc agactgtggg catagagacg cccgccagta acgttcttgg
    43321 cacctgcgga attgccacgc tcgataacta acacttgcgc cccttcgcgg gcgagcacca
    43381 gtgcggcaac cgaaccggca agccctgcac cgacgatgat ggcgtcaaag atatcttcgg
    43441 acataagagc tccctgtcag cggcagagcc gtaatgcagc cctgccagag tggatcaacg
    43501 cgctaaagct gcggtcagtg ccggaaggat cttcacggca tcgccaacaa tgccgtaatc
    43561 cgcgtactgg aagatcggcg catttttatc tttattgatg gcgaaaatgg tttgcgacgc
    43621 gttagcgcca accatgtgct ggatctaccc ggagatcccc accgccaggt acagttcagg
    43681 tttcagcatc aggttggaga taccgacata gcgttcgtgc tccatccatt tttcgttttc
    43741 tgccaccgga cgagaacagg ccaactccgc acctatcgcc ttgcaaagct gttctgccag
    43801 cgcaatgttc tctttgctgc caatgccgcg accgacgctg accaccagac gggctttgtc
    43861 gagatcgacg ctgttgctct ggcgcgcctg ggttgccgtg cgggtaatcg ccacagccgg
    43921 agcctgccac tccacggtgt gcgtttcgcc agtgcgtgac gcgtctggct gagccgcatc
    43981 gaacgtgccg ctgctgatgg tcagtaccgc atacggcgtg gcaatgcgtt cttcgccaat
    44041 cgccagaccg ccgtaaacca tgtgtttcac tgttgcttta ccgtcctgta cgctgacggt
    44101 gctggcatcg ttagacaccg ccgctttaag gcgataaccc agttttgccg ccagtaattt
    44161 gccgcgacgg gtgtttggca acagcaccag accgtcagcg ccgtgctggc gaatagtgtc
    44221 agccatgaca ccggcgtaat cttcgatcat ccgatcgtcc ggtttgccgt ttaatttcca
    44281 gacatgatta gcgccgagct ggattgcctg tgcgccgtcg gcatcattga ggacaaaggc
    44341 attgatttga ttagctaaag cctgcgcacc gttcatcagt tccggcagac gagagggggt
    44401 atcgctgaat acccagactt gagaaaacgt gttcatagca tcccctgtaa ttaaatgact
    44461 ttgcgaagat tttcagcaaa tgcggcgatc tgttcttcgc cgtcgccttc aatcacgatg
    44521 cgctgacgct cgcgctgttt cggcgcggca acctgttgtt cagaccaggc gtccacggcg
    44581 ttaaaaccaa tatccgccgc cgaccatacc tggactggct ttttcgccgc gccgagaatg
    44641 gctttcatcg aaggaatttg tggggagttg atatcagtgg aaacagcaac aaccgcaggc
    44701 agcggaatgc ttaaggtttc ggtttcatct tccagttcgc gctcaacggt gagggtatct
    44761 gccgtcaggg agataatttt gctgacgccg ttaactgccg gaatattgag gatttcgccc
    44821 accagcagac caacctgctg ggcataaagg tcggaagaac catcgccaca gaggatcaga
    44881 tcaaagcctg ctttctgggc ggctgcagcc agtgcgctcg ccgtttgttg cggcagtgcc
    44941 tgctcgaact ggtcatcaat caccacaatc agttcatccg ggccgcgcga tagcacatct
    45001 ttacgccctt tggcgttggt cagggcttta ccgcccacac ttaaggctgt cacctgcgcc
    45061 tctgctgcct gttgctttag ctggcaagcc gcttcaatag cgttgagatc gtattggctt
    45121 attttggcat cggctttgct gaagtctaat gaaccatcag cattattgac cgcaatatcc
    45181 tgttcatcag gcacgcactt atagcaagta ataatcttca ttgcatctcc agaaatcatg
    45241 aaggaaacgt cgttcatatc ggtggtgtta attacagata tcacgccctt taatttggaa
    45301 ataaaacttt aatcaccaat attgaaagtc agatgctatt tttttaccca caaaagaata
    45361 taacaaactg attaataacc aaaattaacg aaaaacgccc tctgttcact cgaaacaaaa
    45421 atgtgatacc aatcacagaa tacagcttat tgaataccca ttatgagtta gccattaacg
    45481 cgtccacgat gttaataata attatattaa atgttaacaa aaataaaaca aacgggaacg
    45541 caaaataaat attcgttttc acagtggaat taactcatga agaatgaaaa gagaaaaacg
    45601 ggaatagaac cgaaggtttt ctttccgcca ttaataatcg tcggcatact ttgttggctt
    45661 acagtcagag atctggatgc ggcgaatgtc gttattaatg ctgtattcag ttacgtcacc
    45721 aatgtatggg gatgggcatt tgaatggtat atggtggtga tgcttttcgg ttggttctgg
    45781 ctggtgtttg gcccgtatgc caaaaagcgt ttaggtaacg aaccgccaga atttagcacc
    45841 gccagttgga tctttatgat gttcgcctcc tgtacgtctg ctgccgtact gttctgggga
    45901 tcgattgaga tctactacta catctccacc ccgccgtttg gcttagaacc gaactcgaca
    45961 ggagcgaaag agttggggct ggcttacagc ttgttccact ggggacctct gccgtgggcc
    46021 acttacagct tcctttcagt cgccttcgct tacttcttct ttgtccgcaa aatggaagtg
    46081 attcgcccca gctcgacact ggtgccgctg gcaggtgaaa aacacgccaa agggttgttc
    46141 ggtaccatcg tcgacaactt ctatctcgtc gccttgatct tcgcgatggg taccagtctg
    46201 ggccttgcca cgccgctggt gaccgagtgt atgcaatggt tgtttggcat tccgcatacc
    46261 ctgcaactgg acgctatcat cattacctgc tggattatcc tcaacgccat ttgcgtcgct
    46321 tgcggtctgc aaaaaggggt acgtatcgcc agtgacgtgc gtagttacct gagcttcctg
    46381 atgctgggtt gggtgttcat cgtcagcggt gccagcttca tcatgaacta cttcaccgat
    46441 tcggtgggga tgttgctgat gtatctgccg cgcatgttgt tctataccga tcccatcgct
    46501 aaaggcggct tcccgcaggg ctggaccgtg ttctactggg catggtgggt gatttacgcc
    46561 atccagatga gtatcttcct cgcccgcatc tcccgtggtc gtactgtgcg tgaactgtgc
    46621 ttcggcatgg tgatggggct gacagcatcc acctggatcc tgtggaccgt gctcggtagt
    46681 aacactctgc tgttgatgga taaaaacatc atcaacattc caaatctgat cgaacagtac
    46741 ggtgtggcgc gcgccatcat cgaaacctgg gccgctctgc cactcagcac cgccaccatg
    46801 tggggcttct tcatcctctg ctttattgcc accgttacgc tggttaacgc ctgctcttat
    46861 accctggcga tgtctacttg ccgcgaagta cgcgatggtg aagaaccacc gctgctggtg
    46921 cgtatcggtt ggtcaattct ggttggcatt atcggtattg ttctgctggc gctcggcggc
    46981 ctgaaaccga ttcaaaccgc cattatcgcc ggaggatgcc cgctgttctt cgtcaacatt
    47041 atggtgacgc tctcctttat taaagacgcg aaacagaact ggaaagatta attaaccccc
    47101 aaaatatcaa gaggttgaaa gatggatttt aatttaaatg atgagcagga actgtttgtc
    47161 gccggtatcc gcgaactgat ggccagcgaa aactgggagg cctattttgc cgagtgcgac
    47221 cgtgacagcg tctacccgga acgttttgtc aaagcactgg cggatatggg tatcgacagt
    47281 ctgctgatcc ctgaagagca cggtggtctg gacgcggggt ttgttactct cgccgccgtg
    47341 tggatggagc tgggacgtct gggggcacca acctatgtac tgtaccagct gccgggcggg
    47401 ttcaatacct tcctgcgcga aggcacacaa gagcagatcg acaagattat ggctttccgc
    47461 ggcaccggta agcagatgtg gaactcagcg attaccgaac cgggcgcggg ctccgacgtg
    47521 ggtagcctga aaacgactta tacccgtaga aatggtaaga tttatcttaa tggtagtaag
    47581 tgttttatta ccagtagcgc ctacaccccg tacatcgtgg tgatggcgcg cgacggggct
    47641 tctccggaca aacctgtcta caccgaatgg tttgttgata tgagcaaacc gggcatcaaa
    47701 gtgaccaaac ttgagaagct cggtctgcgt atggatagct gctgtgaaat cacctttgac
    47761 gatgtggaac tggacgagaa agacatgttc ggtcgggaag gtaacggctt taaccgcgtc
    47821 aaagaagagt tcgaccatga acgtttcctg gtagccctga ccaactacgg tacggcgatg
    47881 tgcgcctttg aagatgctgc gcgctacgcc aaccagcgcg tgcagtttgg cgaggctatt
    47941 ggtcgtttcc agttgattca ggaaaaattc gcccacatgg cgatcaaatt aaactccatg
    48001 aaaaacatgc tgtatgaagc agcgtggaaa gcagacaacg gcaccatcac ctctggcgat
    48061 gcagcgatgt gcaaatactt ctgcgccaat gcggcatttg aagtggtgga tagcgcaatg
    48121 caggtgctgg gcggtgtcgg gattgcgggc aaccaccgca tcagccgctt ctggcgtgac
    48181 ctgcgtgtag accgcgtttc cggaggatct gacgaaatgc agatcctgac gctgggtcgt
    48241 gcggtgctga agcaataccg ctaagtccgt gcgctgcctg atgcgacgct gacgcgtctt
    48301 atcaggccta cgggagcacg aatgtaagcc ggataaggcg tttacgccgc atccggcagt
    48361 catgcgctac ataaattttt caggagctaa taatggatca tctacccatg ccgaaattcg
    48421 gaccgctggc cggattgcgc gttgtcttct ccggtatcga aatcgccgga ccgtttgccg
    48481 gacaaatgtt cgcagaatgg ggcgcggaag ttatttggat cgagaacgtc gcctgggccg
    48541 acaccattcg cgttcaaccg aactacccgc agctctcccg ccgcaatttg cacgcgctgt
    48601 cgttaaatat tttcaaagat gaaggccgcg aagcgtttct gaaattaatg gaaaccaccg
    48661 atatcttcat cgaagccagt aaaggtccgg cctttgcccg tcgtggcatt accgatgaag
    48721 tactgtggca gcacaacccg aaactggtta tcgctcacct gtccggtttt ggtcagtacg
    48781 gcaccgagga gtacaccaat cttccggcct ataacaccat cgcccaggcc ttcagtggtt
    48841 acctgattca gaacggtgat gttgaccagc caatgcctgc cttcccgtat accgccgatt
    48901 acttttctgg cctgaccgcc accacggcgg cgctggcagc actgcataaa gtgcgtgaaa
    48961 ccggtaaagg cgaaagtatc gacatcgcca tgtatgaagt gatgctgcgt atgggccagt
    49021 acttcatgat ggattacttc aacggcggcg aaatgtgccc gcgcatgagc aaaggtaaag
    49081 atccctacta cgccggttgc ggtctgtata aatgtgccga cggctacatc gtgatggaac
    49141 tggtgggcat tacccaaatt gaagagtgct ttaaagatat tggcctcgca catctactcg
    49201 gtacgccgga aatcccggaa ggcactcagc ttatccaccg tatcgaatgc ccttacggcc
    49261 cactggtaga agaaaaactc gatgcctggc tggcggcaca taccatcgca gaagttaaag
    49321 aacgctttgc cgaactgaat atcgcctgtg ccaaagtact gaccgtaccg gaactggaaa
    49381 gcaatccaca gtatgttgcc cgtgaatcaa tcactcagtg gcaaacgatg gatggtcgca
    49441 cctgcaaagg gccgaacatc atgccgaaat tcaaaaataa ccccggacaa atctggcgcg
    49501 gaatgccctc acatggcatg gacacggctg ccattttgaa aaatatcggc tacagcgaaa
    49561 acgacattca ggagttggtc agcaaaggtc tggccaaagt tgaggactaa acgcgtggcg
    49621 tcagtcggct tgcctgacta gcgcccgccc aataacaaac gaacaaaaat ggatagaggt
    49681 gcaatggata tcattggcgg acaacatcta cgtcaaatgt gggacgatct ggcggacgtt
    49741 tacggtcata aaacggcgct gatttgtgaa tccagcggcg gagtcgttaa ccggtatagt
    49801 tatcttgagt taaatcagga gattaaccgc acggcaaacc tgttttatac gctggggatt
    49861 cgcaaaggca acaaggttgc actacatctc gacaactgcc cggaatttat cttttgctgg
    49921 ttcgggctgg caaaaattgg cgcgattatg gtgccgatta acgcccgcct gttacgcgag
    49981 gaaagcgagt ggatcttgca aaatagccag gcgtgcctgc tggtgaccag tgcgcaattt
    50041 tatcctatgt atcaacagat tcagcaggaa gatgccactc aattgcggca catttgcctg
    50101 acagatgtgg cacttcccgc tgatgatgga gtgagttcat ttactcaact gaaaaatcaa
    50161 caacctgcca ccttgtgcta tgcaccgccg ctatcgactg acgatacggc ggaaattctc
    50221 ttcacctccg gcaccacctc ccgaccgaaa ggtgtggtga ttacccatta caacctgcgc
    50281 ttcgctggat attactccgc ctggcagtgt gcactgcgtg acgatgacgt ctacatgacg
    50341 gtaatgcctg cgtttcatat cgattgccag tgtactgcgg cgatggcggc gttttctgcc
    50401 ggggccacct ttgtgctggt cgagaaatac agcgcccgcg ccttctgggg acaggcgcag
    50461 aagtaccgcg ccaccattac cgaatgtatt ccgatgatga tccgtacgtt gatggtgcag
    50521 ccgccttcag cgaacgatcg gcaacaccgc ctgcgggaag tgatgtttta tctcaacttg
    50581 tcggagcagg aaaaagacgc attttgtgaa cgcttcggcg ttcgcttgct gacgtcttat
    50641 gggatgacgg aaaccattgt gggcattatc ggcgatcgcc ctagcgataa acgacgctgg
    50701 ccgtcgattg gtcgggcggg gttttgctac gaagcggaga tccgcgacga tcacaatcgc
    50761 ccgctcccgg caggtgagat cggtgaaatc tgtattaaag gcgtaccagg gaaaaccatc
    50821 ttcaaagagt attttctcaa cccgaaagcc actgcgaaag tgctggaagc cgacggctgg
    50881 ctacataccg gcgataccgg ataccgcgac gaagaaggct ttttttattt cgtcgatcgc
    50941 cgctgcaata tgatcaaacg cggtggcgag aatgtctcct gcgtggagct ggaaaatatc
    51001 atcgccaccc atccgaaaat tcaggacatc gtggttgtgg gtattaaaga ttcgattcgc
    51061 gatgaagcca tcaaagcatt tgtggtgctg aatgaaggtg aaacattgag cgaagaggaa
    51121 ttttttcgct tctgcgaaca aaatatggcg aaatttaaag tgccctctta tctggagatc
    51181 agaaaagatc tgccacgtaa ttgctcgggg aaaataatta gaaagaatct gaaataaatg
    51241 aaacgtcagg gaactaccct gccagctaat aatcacgccc taaaacaata tgcatttttt
    51301 gcagggatgt tgtcgtccct aaaaaagcaa aaatggagaa aaggaatgag tgaatcatta
    51361 catctgaccc gcaatggatc aattctggaa attacccttg atcgcccaaa agcgaatgct
    51421 attgatgcaa aaaccagctt tgaaatgggc gaagtatttc taaatttccg tgacgatccg
    51481 caattacgtg tcgccattat taccggtgcc ggagagaaat tcttttccgc gggctgggat
    51541 ttaaaagcgg cagcagaagg cgaagcaccg gatgctgact ttggcccggg tggttttgcg
    51601 ggattaaccg aaattttcaa tctcgacaaa ccagttatcg cagctgtgaa cggctacgcc
    51661 tttggcggcg gtttcgaact ggcgctggcg gcagatttta ttgtttgtgc cgataacgcc
    51721 agcttcgccc tgccggaagc caaactgggc atcgttcctg acagcggcgg tgtgctgcgt
    51781 ctgccgaaga tcctgccgcc taccatcgtc aatgaaatgg tgatgaccgg cagacgaatg
    51841 ggcgcagaag aggcgctgcg ttgggggata gtcaaccgcg tggttagcca ggcggaactg
    51901 atggataacg cccgcgaact ggctcagcag ctggttaaca gcgccccgct ggcgattgcg
    51961 gcgctgaaag agatctaccg caccaccagc gaaatgccgg tagaagaagc gtatcgctat
    52021 attcgcagcg gcgtgttgaa acactatcca tcggttctgc attcggaaga tgccattgaa
    52081 gggccgctgg cgtttgccga gaagcgcgat ccggtgtgga aaggacgtta acaaccgtga
    52141 gctattacgc ctttgagggg ttaattccgg tggttcaccc gacggcgttt gtccatccca
    52201 gtgccgtctt gattggcgat gtgattgtgg gagccggtgt ctacatcggc ccactcgcct
    52261 cactgcgtgg tgactacggg cggttgatcg tgcaagcggg agccaatatt caggatggct
    52321 gcattatgca tggctactgc gacactgaca ccatcgttgg ggaaaacggc catatcgggc
    52381 atggagcgat cctgcatggt tgtgtgattg gtcgcgatgc attggtcggg atgaacagcg
    52441 taattatgga tggcgcggtc attggcgaag agagcattgt tgccgcaatg agctttgtca
    52501 aagcaggctt tagtggcgag aaacgccagt tgttgatggg tacgccagcc cgcgctgtac
    52561 gcagtgttag tgacgacgag ttacactgga aacggttgaa taccaaagag tatcaggatc
    52621 ttgtcgggcg ctgtcatgca tcgttacatg aaacgcagcc actgaggcaa atggaggaaa
    52681 atcgcccccg tttgcagggg acgacggatg tgacgcctaa acggtgatct tgccggatgc
    52741 gacctggttg ctggataaga tgctacaaca tcgtatctgg caagcaggac gcccggtatc
    52801 aacgagataa aattaacgac gcatactctt tgacaacatc atctgccact tctgctcgcg
    52861 gttaagttcc gtgagcggaa cggcaggaat acgcggtgtc tttcttacgc cgactaattt
    52921 ttcccgactg ttattgcgca ggcgcgcgta aatttgctca tcgatatcga ccactttaac
    52981 cagtcgctga cactggcatc cccgcccttc cagcttatta gggatcatct taacttcgca
    53041 gcttatttct gtgacttccg acagaatata agtcagggta ttaaccgcct tgctgtgttc
    53101 aatatcgaaa tgaatagaga tctctcttgc tatcacccac cgattttcag ccatcatcca
    53161 ttcggcgatt aacaagtaga gtggtttttc aacatatcct tcacacatac gaactccagt
    53221 ttcacaaatt aaaagtctag ctatgactcg ttttgaagca attaaacaag gccatattaa
    53281 aattgtggat atatcgatag tatgcaactt caccgtcgat aagtgtgagc taaatcctgc
    53341 ttatgtcatc aaaaatatcg attccccaaa agatttactt aatgggcaaa aaaaaacggt
    53401 cctcatcaga gaaccgtatt agttattcga tcaaattagc ggatgaaaaa tatccgccac
    53461 gacacgctat tatttgatct gtgcgtgcat ttcctgcacc gaaattactt tttcagtcgc
    53521 atcggcattc agcgccatcg cggtagcgaa accaccgttc agggtggtgt cataatgcac
    53581 tttatattgc agcgcactgc gacggatcac gcgggagtct tcaatcgcac gacggcctga
    53641 ggtggtgttg atgatgtagg tatattcgcc attcttgata cggtcctgaa tgtgcggacg
    53701 gccttcatgc accttgttta ccagacgcgg attgataccc gcttcgccca gcacaatcgc
    53761 cgtgccgtgg gtcgcatcca gctcgaagcc ctgtttcagc agttttgccg ccaggtccac
    53821 cacgcgttct ttatcgcctt cgcgcacgga aagcagcgca cgaccgtgtt tcttcatggt
    53881 ggagttgctg cccagctgcg ctttggcgaa cgcttcagcg aaggtgcggc ccacgcccat
    53941 gacttccccg gtagagcgca tttctggccc taacagcggg tcaacgcctg ggaatttatt
    54001 gaacggcagc accacttctt tcaccgagta gtacggcgga ataacttctt tggttacgcc
    54061 ctgctcagcc agcgatttgc cagccatcac acgcgccgcc acttttgcca gcggtacgcc
    54121 ggtggctttg gagacgaacg gaacagtacg cgccgcacgc gggtttactt caatcaggta
    54181 aacttcgttg tttttcaccg caaactgcac gttcatcaga ccgcgcacct gcaattcgaa
    54241 ggccagtttc tgcacctgct ggcgcatcac atcctgaatt tcctgactta aggtgtacgc
    54301 tggcagcgaa catgcggagt caccggagtg cacacccgcc tgctcgatgt gctccatgat
    54361 gccgccaatc agcaccattt cgccgtcgca gatggcatcc acgtcaactt ctactgcgtc
    54421 atcaaggaaa tgatccagca acactggcgc atcgttagac acgctaaccg ccgtctggaa
    54481 gtagcgacgc aggtcagctt cgtcatagac gatttccatc gcccgaccgc cgagaacgta
    54541 agacggacgc accaccagcg ggtagccaat ctcttttgcc ttctcaactg ccatttcaat
    54601 agcagtaacg gtggcgttcg ccggttgttt cagtttcaga cggtcaaccg catgctggaa
    54661 gcgttcacgg tcttccgcac ggtcgatagc atccgggctg gtgccgataa ccggtacgcc
    54721 agcggcttcc agtgcacgcg ccagtttcag cggggtctga ccgccgtact gaacgataac
    54781 gcctttcggc ttctcaatac gcacgatttc cagcacatct tccagagtta ccggctcgaa
    54841 gtagaggcga tcggaagtgt cgtagtcggt agagacggtt tccgggttac agttaaccat
    54901 aatggtttcg taaccgtctt cacgcagcgc cagcgaggcg tgtacgcagc agtagtcgaa
    54961 ttcgataccc tgaccgatac ggtttggacc gccgccaagc accatgattt tttcacggtc
    55021 ggtagacgga ttcgcttcgc actcttcttc ataagtggag tacatgtaag cggtgtcggt
    55081 ggcaaactct gccgcacagg tatccacgcg cttgtagacc gggtgcaggt catattggtc
    55141 acgcagctta cggatttccg cttcgcgtac gcccgccagt tttgccaagc gcgcatcggc
    55201 aaagcctttg cgtttcagct ggcgcaggaa ttcagcgttc aggccagtga tgcccacttc
    55261 cgccactttc tcttccagac gcaccagttc ttcaatctgt accaggaacc agcggtcaat
    55321 gttagtcagg ttgaagacgc cgtccacaga caggcccgcg cggaaagcat cggcgatgta
    55381 ccagatacgc tctgcgcctg cgtctttcag ttcgcgacgg attttggtta acgcttccgg
    55441 gtcatccagg ctcactttcg ggtcgaagcc agttgcgccc acttccaggc cgcgcagcgc
    55501 tttttgcagg gattcctgct gcgtgcgacc aatcgccatc acttcgccaa ccgatttcat
    55561 ctgagtggtc agacggtcgt tagcaccggc gaatttttcg aagttgaagc gaggaatttt
    55621 agtaaccaca tagtcgatgg acggctcgaa ggaggccgga gtacgtccgc cagtgatgtc
    55681 gttcatcagt tcgtctaggg tgtaacccac cgccagtttc gccgccactt tagcaatcgg
    55741 gaaaccggtc gctttcgacg ccagcgccga agaacgggac acgcgtgggt tcatttcgat
    55801 aacaatcagg cgaccgtttt tcgggttcac cgcaaactgg acattggaac caccggtttc
    55861 aacgccgatt tcacgcagca ccgccatcga ggcgttacgc atgatttgat attctttgtc
    55921 ggtcagcgtt tgggctggcg cgacagtgat ggagtcaccg gtgtggatgc ccatcgcatc
    55981 aaagttttcg atagagcaga cgatgatgca gttgtcgttt ttatcacgca ccacttccat
    56041 ctcgtactct ttccagccga tcagcgactc atcaatcagc aactctttgg tcggagagag
    56101 atccagaccg cgggcgcaaa tttcttcaaa ctcttcacgg ttataagcga taccgccgcc
    56161 gctaccgccc atggtaaagg atgggcgaat aatgcacggg aagcccacgt cagcggcaac
    56221 agccagcgct tcttccatcg tgtgtgcgat accggaacgc gcggtttcca gaccaatttt
    56281 cttcatcgct acgtcgaaac gacggcggtc ttctgcttta tcaatcgcat cggcagtggc
    56341 accaatcatg gtgacaccga actcttccaa cacgccctga cgttccagct ccagcgcgca
    56401 gttcagcgcc gtctgaccgc ccatggttgg cagcaccgcg tccgggcgct ctttttcaat
    56461 aatcttgcgt accacttccc agtgaatcgg ctcgatgtag gtcgcatcgg ccatttccgg
    56521 gtcggtcatg atggtcgccg ggttggagtt caccagaatg acgcggtaac cctcttcgcg
    56581 cagggcttta catgcttgcg cgccagagta gtcaaactca cacgcctgac cgataacaat
    56641 cgggcccgca cccagaatca ggatactttt tatatctgta cgttttggca tggctctttt
    56701 actcctgatt acttagcggt tttacggtac tgctcaatta acttgataaa gtggtcgaac
    56761 aacggcgcgg cgtcgtgtgg accagggctg gcttcagggt gaccctggaa gctgaacgcc
    56821 ggcttatcga tgcgatgaat gccctgtaac gtaccgtcga acagggattt atgcgtgaca
    56881 cgcaggtttg caggtaatgt tgcttcatcc accgcaaaac cgtggttctg ggcggtgatc
    56941 atcaccacgt ttttctccac atctttaacc ggatggttgc cgccgtggtg accaaatttc
    57001 attttgacag tcttcgcacc gctcgccagc gccagcagct gatgaccgag acagatgccg
    57061 aataccggaa tatcggtttc gaggaacttc tggatggcgg taatggcgta atcgcacggg
    57121 gccgggtcgc caggaccgtt ggagaggaag atgccgtctg gattcatttt cagcacatct
    57181 tccgcagaag tttgcgccgg aacgatggtc aggcgacagc ctctatccac cagcatccgc
    57241 aggatgttgc gcttggcacc aaaatcataa gccacgacgt ggaacggcag ctcgtcttct
    57301 tttttcgctt ctggcaggcc gccggtcaac gtccagctcc cttgtgtcca gctataggct
    57361 tctgcggtgg tcacttcttt tgccagatcc atgccgttca gacctgggaa cgcgcgggct
    57421 ttttctaacg ccagcgccgc atccgggtta tcgcctgcga taatgcagcc attctgtgcg
    57481 cctttctcgc gcagtaaacg cgtcagctta cgggtatcga tatcggcaat cgccacgatg
    57541 ttatggcgct tcaggtaaga agagaggtct tcggtattac ggaagttgct ggcaatcagc
    57601 ggcaggtcgc gaatcaccag accttgtgca tgtacctgag aagattcttc atcggcgtca
    57661 ttagtgccga cattgccaat atggggataa gtaagagtaa cgatttgacg agaataggaa
    57721 ggatcagtga ggatttcttg ataaccggtc attgaagtat tgaaaacgac ttccccaacc
    57781 gccgaacctg ttgcccctat ggcccgaccg tgaaactggg ttccgtcttc cagaaccaat
    57841 agcgctgact taatcaaaac accctccaga gaatactcac tcactttatt tgcatattaa
    57901 ttcacagtga tgacgtaaat caatgcaaat ctgcttaccg acgaatttct ggcaaacggc
    57961 ggcattctgg agatatgggc gctaaaagtc aacttaatgg caggattttt tatattattt
    58021 tgtggctcat gcatgtttta caggcattaa gcgacaaaaa catcatctaa cctgttctgg
    58081 aaaacgcttg cgcagcaaga agattgcatg aaataaaaac gagcaggaaa aagtggacca
    58141 aatggtcaaa atttacatta aaacaacaaa aatagaagac gaaacatgta atttttggca
    58201 gttatagaaa atttaaaagc ataacaccaa aaataaaagg gctaaaaatt aaagcccttt
    58261 ataattaata aattaaatgt gttacttttg catcataata aacattttgt ggttacaaat
    58321 tattgagatc aagcacatct cgcatatcaa aaagaccact ttccttacca ctcaaccaca
    58381 aagccgatct taccgcaccg ttagcaaatg tcatgcggct ggacgcctta tgggtgatct
    58441 ccagacgctc gccaatatcg gcaaacatcg cggtatgttc gccaacgatg tcacctgcac
    58501 gcacggtagc aaaaccaatg gtacctggca cacgttcacc ggtatggcct tcacgactgt
    58561 agaccgcgca atctttcaga tctttatcaa gggcgtgggc gatcgcctct cccattgcca
    58621 gtgcggtgcc tgacggcgca tcaactttat gtctatgatg tgcttcaata atttcgatat
    58681 cggtgtagtc acccatcact ttggctgctt tctccagcag cttaagcatg acgttaacgc
    58741 caacgctaaa attggcggca aagacaatcg caatatcggc ggcggcgtca cgaattgcct
    58801 gtttaccggc ttcgtcaaac cccgtagtgc cgatcaccat ccctttgcca tgctgccgac
    58861 aaaaagcgag atggttcagc gtaccttccg gacgggtaaa atcgataaac acatcaaaat
    58921 catcttttat cgcatcaaga ctgctttgca cggtaacgcc tgttttccct gcaccagcca
    58981 gctcaccggc gtcgctgccc agtaaagaag atccttcgcg ctccagcgcc gcgcccagct
    59041 gcacgccttc taatgccagt gccgcctgaa tcaactggcg gcccatacgc cccccggctc
    59101 ccgcgatggc aacgcggatg tttgcatcat gcatagctat tctcttttgt taatttgcat
    59161 agaccgtttt cagagtaacc agcgcatcgc tacgccgcca gccaaaaacg ctgataatta
    59221 gaaattaatg ataaacaggg agggatatca gtgaaaggca tgagtcgatg aatgactgca
    59281 tgccgataac atgtgacagg ttacggcacc agagccagca cctcagccac ccactgttgg
    59341 aagcctttta cattcagatc caatgccacc tgtacattgg ctggtttgcc caggcaaccg
    59401 tcgatatcaa ccaccgtcgt gcctgaggta aattcgcctt gagtttctac cgcgacaaaa
    59461 cacggcttga gggtgaataa gtccgggcgc accagccagg cgatggcgca gagatcgtgc
    59521 attcgcagac cgctttgcat actgccgcta cggtagtggc taaacagggc gtggagcatt
    59581 tttccggtgc gattcaactc cggtaaagta gcgagatagt caggggttaa tattgcctga
    59641 ttggtgacat ccagaccgca catgacaatt tcgatgccgc tacggaagac acaggcagcc
    59701 gcttctggat cggcagcgat attaaactcg gcatttggtg tacagttgcc gcgaccagca
    59761 gaaccgccca tgatcaccag acgacgaata tacggtttac attccgggca ttgtaaaagt
    59821 aacagcgcaa tattggttaa cgggccgatg gccaccaggg taacgggctc tggtgcacgc
    59881 atcagggcat cccgaatcgc cagaaacgcc ggtatcccga gtggctttcg gttgtgctca
    59941 acaaagtcgt agccagccat tcccgattcg ccgtgcacag atgctgcatc acgcggtgcg
    60001 cgtaccagcg gcacagcggc tccctgggcg agcggaatct ccgcattcca gaaatgcagc
    60061 agttgcaggg cattgcgggt agttttctca accgagacat tacccgcgac ggtggtcatc
    60121 agttgcaggt cgagttcggg ggcaaaaatc gcggcggcaa tggcgacggc atcgtcaatg
    60181 ccggggtcag tatcgaggaa gataggtaaa cgcatgtttt cttcataaaa aatgccggta
    60241 acaagaccgg cattttcgca taacttaggc tgctaatgac ttaatcgact tcacgaatat
    60301 cgacacgcag ctctttcggc acttcgaaaa caatattttc ttcacggcct tccagcggaa
    60361 tggcttcacc accacccaac tgctgcaaac gtgccaccac attctgcacc agaatatccg
    60421 gagccgatgc gcccgcagtc acgccgacgc atttaacctc tttcacccac tcttcctgga
    60481 tatctttcgc atcgtcaatc aaaaacgcgt gtttgcccat acgctgggcc agctccgcca
    60541 gacggttgga gttggaggag tttttcgaac cgaccaccaa cacaacttcc gcctgttctg
    60601 ccagggcgcg taccgcttcc tgacggttag tcgtggcgta gcagatgtca tctttgcgcg
    60661 gaccgacaat tttcgggaag cgtttacgca gcgcgtcgat cacatcagac gtgtcatcca
    60721 ccgacagcgt ggtttgggtc ataaaggaga gcttctcttc gtttttgacc gtcagtttcc
    60781 acacatcgtc tggcgattcg actagataca ttcccccttc ggggttgctg tactgcccca
    60841 tcgtcccttc cacttccggg tgcccggcgt ggccgatgag aatagattct tcgccacggc
    60901 gactggcgcg ggcaacttcc atatgcactt tggtcaccag cggacaggtg gcatcaaaca
    60961 ccgtcaaatc gcggcttttc gcttcgttac gaaccgcctg agaaacaccg tgtgcggaga
    61021 aaatcaggat cgcgccgtcc ggcacttcgc taatctgctc aataaagata gccccacgct
    61081 cgcgcaagct atcgaccacg tagcggttat gcaccacttc gtgacggaca tatatcggtg
    61141 cgccgtaaat ggccagcgcg ttttcaacaa tgctgatagc gcggtctacc ccggcacaaa
    61201 aaccacgcgg gttggccaac aggatctgca tgttacgcct ccagtgccgg atcgatttcc
    61261 agcacttcaa tatcaaaatg aacggtctgc ccggccagcg gatggttgaa atcaacggta
    61321 atggagtcgc cgttaatttc gcggatcaca ccaggcatct cactgccatc cattgcggta
    61381 aaaagcatga ttgcgccaat ttctggctcg cctgcatcca taaattcacg gcgggagaag
    61441 tactgaatca ggtccggtga cggcacgcca aacgccgcat ctggctccag cgagaaggtg
    61501 gttttgtcgc ccactttcag ccccagcagg tgttgctcca gcccttcaga aagagaagca
    61561 tcgcccaggc ggaacagcgc cggtttaccg ttgttgcgag tagactcggc ggtggtgcca
    61621 tcgtcgagtt ttagcgtgaa gtgcaccagg acggcgctat tgctctgtac agattcagac
    61681 atgcaggttt gctcttattt ttgaggattt ttgccggatg cggcctacga tttcgtagca
    61741 atctgtaggc cgcatccggc aggatttatt attgtttttt cgctttggaa ggtaaaaacc
    61801 cttccagcac gatcagtgcc gcaccgacac aaatggcagt atcggcaagg ttgaaggtgg
    61861 cgaagtgcca gtcgccgacg tagaagtcga tcatatcgac aacgaagccg tgccacaggc
    61921 ggtcgaacag gttgcccagc gcgccgccaa taatcagcgc gtaagcgata ttgtttagct
    61981 tctgcgtggc cttcgagcga tacatcatca ctgccaggag cacgctaata ccaatcgcaa
    62041 taccggcaaa gaaccaacgc tgccagccgc cgctatcggc aaggaaacta aacgccgcgc
    62101 catagttacg cgcataatgc agattaagcg acgggaacag cgggaccgta tcccccagag
    62161 caaagttctg gaggatcagg tatttgctgc ccagatcgat aatcagcacg actaccacca
    62221 gccacagcca gcgtagccct gttgaacaga tcgattgact catcaggcaa acttacgttt
    62281 ttcaccgtca ccggcgacgt tgctgacaca gcggccgcag atttctgcgt gttccgccac
    62341 cttgccgaca tcctgggtgt agtgccagca gcgtgggcac ttctcacctt cggctttact
    62401 caacgcgact ttcagccctt tgagtacttc gctctgctga gcatcagcag gtgcgtcgtt
    62461 atagtctgca acggtagcgc cggaggtcaa caggacaaat cgtaattcat cgcccagcgc
    62521 ggtcagtttc gccgccagtt ccggttctgc atacaaggtt actgccgctt ccagcgagcc
    62581 acccactttc ttgtcggcac gcgcttgctc aatgactttg ttcacttcgc cacgcacttt
    62641 caacagctcg tcccagaacg catcgttcat tgcttcactg tctgccagac caaacaggcc
    62701 ttcgtaccac tcaccggtga agacgtattt ttcacgttcg cccggcagat agccccacac
    62761 ttcatcagcg gtgaaggaga ggattggtgc catccagcgc accagcgctt cagcgatgtg
    62821 atacagcgca gtctggcagc tacgacgcgc cacgctatcc gctttggcgg tgtactgacg
    62881 gtctttgatg atgtcgaggt agaaggaacc catctcaacg gagcagaagc gcatcagacg
    62941 ctgtaccact tcgtggaaat cgtatgcttc gtacgccttg aggatgtctt cctgtgccgc
    63001 tttcgcacaa cctacggccc agcgatccag taccaccatc tcttccggtt tcaccatatc
    63061 ttttgctgga tcaaaaccat tcaggtttgc cagcaggaag cgcgcggtgt tacggatacg
    63121 acgatagcta tcggcagcac gtttcaggat ctcgtcagaa acggccattt caccggtgta
    63181 gtcggttgat gccacccaca gacgcagaat atccgcgccc agtttgttca tcacatcctg
    63241 cggcgaaacg gtgttgccga tggatttaga catcttgcga ccctgaccat ccacggtaaa
    63301 gccgtgggtc agtacctgac gatacggcgc tttgcctttc atcgcggtgg agatcattag
    63361 ggaagacatg aaccaaccac ggtgctggtc agaaccttcc agatacatgt ccgctgcgtg
    63421 accggcaaat tccggacgca cgtcaacaac agaagagtgg gtagatccgg agtcaaacca
    63481 tacgtcgaga gtatccggca ctttcacata ctggtcagct tcgtcgccga ggatctcttt
    63541 cgcatcgaga tcccaccacg cctggatgcc gtcaacttca acgcgtttag ccacttcttc
    63601 catcagttcg agggtacgcg gatgcagctc ttccgtgtct ttgtgcacga acagtgacat
    63661 cggtacgccc caggtgcgct gacgggagat acaccagtca ggacggttag caaccatcga
    63721 ctcgatacgc gcctggcccc agtccgggag ccactgcacg cctttgatct ctttcagtga
    63781 ctgcgcacgc agacctttct gatccatgct gacgaaccac tgcggtgtcg cgcggaagat
    63841 gatcggcgtt ttgtgacgcc agcagcacgg atagctgtgc tgcattttct caacgtgcag
    63901 cagcgcgcct ttttcctgca gcagcgcaac gacgatgtcg ttcgctttga agacgttcac
    63961 gccatccagc gtcggataag tgcccggcag ataagtgccg tccgggccaa ccgggttagc
    64021 ggtttccagg ccgtatttct gaccgatcac atagtcgtcc gggccgtggc caggcgcggt
    64081 gtgaacggca ccggtaccgg catccagggt aacgtgatcg ccgaggattg ccggaacgtc
    64141 gaagcccata aacggatggg taaagcgcag cagctcaagc tctgcacctt ttaccgtgcc
    64201 gagaatggtg taatcggtca cgccgatacg ctgcattacg ctttcaacca gatctttcgc
    64261 cagaatcacg gcctgaccgt cgatctgcac cagcgcatag tcgaaatcag gtgcaataga
    64321 gattgcgcgg ttcgcaggca gagtccacgg cgtagtggtc cagatcacca gcgagattgg
    64381 gccgttaacg ttgcttacgc caaatttcgc tttcagcgca tcctgatcga ccgcctggaa
    64441 agcgacgtca atggacggag aagttttgtc gtaatactca acttccgctt ctgccagtgc
    64501 agaacggcag tctacgcacc agtgcaccgg cttcgcgcct ttgtgcaggt gaccattgcc
    64561 gatgattttg cccagcgcgc ggatgatgtt ggcttcagtt ttgaagtcca tagtcagata
    64621 cgggtgcgac cagtcgccca gcacgcccag acggataaag tctttgcgtt gaccgtcaac
    64681 ctgggtcgcc gcgtattcgc ggcacttggc gcggaactcg gcggcggtga atttctcacc
    64741 cggcttaccg tattcttgct ctactttcag ctcgatcggc agaccgtggc agtcccagcc
    64801 aggcacatac ggcgagtcat aaccggaaag ccctttggac ttcacgataa tgtctttcag
    64861 aatcttgtta accgagtgac caatatgaat gctgccattc gcataaggag ggccatcatg
    64921 cagaatgaag gtttttttgc cttttttagc cgcacggatg atgccgtaca gatcatcatc
    64981 agtccaacgc gccagcattc ccggttcgcg cttggcgaga tcgccacgca tcgggaaccc
    65041 tgtttccggc aaattcaggg ttgatttata gtcactcatc agattctcgg ttccgtgttt
    65101 cggtttgatt acataacagg cttaagccgg ttttgttagc ccaaaaaatt cgcgggcggt
    65161 taattcatca cgcgcaatct gcgctttcag ttcgtccagc gacgcaaatc gctgctcatt
    65221 gcgtattttt ttacgcagca ctacttgtat atggcgaccg taaaggtcca ttgcaacatc
    65281 taacaaatgc acttccagct gctggcgaat accggcaacc gttgggcgtg ttccgatgtt
    65341 tgccacgccg ggtaacggct tttcaccgag gcccagcact tctaccgcat aaaccccttt
    65401 caccggggaa acctgacggc gtagcggtac attcgccgtc gggaaaccta tagtgcgccc
    65461 taattcatca ccgtggacta cacgcccgga gatggcaaac gggtgcccca gtaaactttc
    65521 tgccagagcc agattgtcat cagccagcgc ctgacgcacg gcggtgctgc tgatacgcac
    65581 accaccttcg caaaaagttt gcgtactggt gatatcgaag ccgtattcca tgccagcttt
    65641 ctgtaataac aagaaatcgc cttcacgacc agcgccaaag cggaaatcat cacctacggc
    65701 aagaaatttt acgcgcaaat gcttcaccaa aagatcgctg atgaaatttt gcgcggttaa
    65761 cgccgcaaaa cgcctgtcga aacgcacgca cagcacgtaa tcaacgccac actctgcaag
    65821 gtaacgcagt ttttcccgca gccgggtcag tcttgccggg gctttatcgg tagcaaacag
    65881 ttccagtggt tgaggttcaa aaagcatcac catcaccggt aagttgcgct tgcgcccttc
    65941 ttcctgcaag ccctgtaaca gcgcgcgatg accgcgatgc acgccgtcga aattaccaat
    66001 agtcagcaca cacccttctt gcggggcctg gctgagatta tgtatgccgc gtatcagctt
    66061 catgtctggc tcaaaacagt gaaaatcgtc cgagtatacc ttgtacagcg gtcaaggtta
    66121 accggcgatt gagtaccgaa atcagaaaga agtggctttt cattgccatg gagcaaatca
    66181 cgggaagaaa ctgaccgcct gctgcaattt ttatcgcgga aaagctgtat tcacaccccg
    66241 caagctggta gaatcctgcg ccatcactac gtaacgagtg ccggcacatt aacggcgctt
    66301 atttgcacaa atccattgac aaaagaaggc taaaagggca tattcctcgg cctttgaatt
    66361 gtccatatag aacacatttg ggagttggac cttggctaat atcaaatcag ctaagaagcg
    66421 cgccattcag tctgaaaagg ctcgtaagca caacgcaagc cgtcgctcta tgatgcgtac
    66481 tttcatcaag aaagtatacg cagctatcga agctggcgac aaagctgctg cacagaaagc
    66541 atttaacgaa atgcaaccga tcgtggaccg tcaggctgct aaaggtctga tccacaaaaa
    66601 caaagctgca cgtcataagg ctaacctgac tgcacagatc aacaaactgg cttaatcgcc
    66661 aatttgctga agctttgtga aaaagcccgc gcaagcgggt ttttttatgc ctgctgcttt
    66721 tgtgatgcgt gagagaacac actgccggtt gccagatagt caaaagagac atgccgttgt
    66781 actatctgaa ctcttcgtgg cttaagaatc gtagaaccat cggctgatac attctgaaca
    66841 agaatatcta ctcaatctcc agactccccc acaaagaata tggatattgt gatacacatt
    66901 gaggtattaa acttaagacc actctatttt tgaattaaat tcctatatac tttaaccatt
    66961 cataaatatc tcctcattag acct
//
LOCUS       DAJTSI010000004        60413 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000004 DAJTSI010000000
VERSION     DAJTSI010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.004"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..316
                     /locus_tag="OO327_000200"
     CDS             <1..316
                     /locus_tag="OO327_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6016883.1"
                     /translation="GTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSN
                     SVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVT
                     YP"
     gene            429..1223
                     /gene="panB"
                     /locus_tag="OO327_000201"
     CDS             429..1223
                     /gene="panB"
                     /locus_tag="OO327_000201"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR6016884.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1235..2086
                     /gene="panC"
                     /locus_tag="OO327_000202"
     CDS             1235..2086
                     /gene="panC"
                     /locus_tag="OO327_000202"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR6016885.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2160..3062)
                     /gene="rpnC"
                     /locus_tag="OO327_000203"
     CDS             complement(2160..3062)
                     /gene="rpnC"
                     /locus_tag="OO327_000203"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR6016886.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3336..3716
                     /gene="panD"
                     /locus_tag="OO327_000204"
     CDS             3336..3716
                     /gene="panD"
                     /locus_tag="OO327_000204"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR6016887.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3720..4949)
                     /locus_tag="OO327_000205"
     CDS             complement(3720..4949)
                     /locus_tag="OO327_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR6016888.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5013..5453)
                     /locus_tag="OO327_000206"
     CDS             complement(5013..5453)
                     /locus_tag="OO327_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR6016889.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5558..6328)
                     /locus_tag="OO327_000207"
     CDS             complement(5558..6328)
                     /locus_tag="OO327_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6016890.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6325..7251)
                     /locus_tag="OO327_000208"
     CDS             complement(6325..7251)
                     /locus_tag="OO327_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6016891.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7360..8022
                     /gene="can"
                     /locus_tag="OO327_000209"
     CDS             7360..8022
                     /gene="can"
                     /locus_tag="OO327_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR6016892.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8063..8599)
                     /gene="hpt"
                     /locus_tag="OO327_000210"
     CDS             complement(8063..8599)
                     /gene="hpt"
                     /locus_tag="OO327_000210"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR6016893.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8805..11195
                     /gene="gcd"
                     /locus_tag="OO327_000211"
     CDS             8805..11195
                     /gene="gcd"
                     /locus_tag="OO327_000211"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR6016894.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11242..12792)
                     /gene="cueO"
                     /locus_tag="OO327_000212"
     CDS             complement(11242..12792)
                     /gene="cueO"
                     /locus_tag="OO327_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCR6016895.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12958..13305
                     /locus_tag="OO327_000213"
     CDS             12958..13305
                     /locus_tag="OO327_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR6016896.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13411..14277
                     /gene="speE"
                     /locus_tag="OO327_000214"
     CDS             13411..14277
                     /gene="speE"
                     /locus_tag="OO327_000214"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR6016897.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14293..15087
                     /gene="speD"
                     /locus_tag="OO327_000215"
     CDS             14293..15087
                     /gene="speD"
                     /locus_tag="OO327_000215"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR6016898.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15125..15487)
                     /locus_tag="OO327_000216"
     CDS             complement(15125..15487)
                     /locus_tag="OO327_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR6016899.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15662..18259)
                     /gene="acnB"
                     /locus_tag="OO327_000217"
     CDS             complement(15662..18259)
                     /gene="acnB"
                     /locus_tag="OO327_000217"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR6016900.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18614..20467
                     /locus_tag="OO327_000218"
                     /pseudo
     CDS             18614..20467
                     /locus_tag="OO327_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20538..21962)
                     /gene="lpdA"
                     /locus_tag="OO327_000219"
     CDS             complement(20538..21962)
                     /gene="lpdA"
                     /locus_tag="OO327_000219"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR6016901.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22287..24167)
                     /gene="aceF"
                     /locus_tag="OO327_000220"
     CDS             complement(22287..24167)
                     /gene="aceF"
                     /locus_tag="OO327_000220"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR6016902.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24182..26845)
                     /gene="aceE"
                     /locus_tag="OO327_000221"
     CDS             complement(24182..26845)
                     /gene="aceE"
                     /locus_tag="OO327_000221"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR6016903.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLFAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27006..27770)
                     /gene="pdhR"
                     /locus_tag="OO327_000222"
     CDS             complement(27006..27770)
                     /gene="pdhR"
                     /locus_tag="OO327_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR6016904.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28311..29684
                     /gene="aroP"
                     /locus_tag="OO327_000223"
     CDS             28311..29684
                     /gene="aroP"
                     /locus_tag="OO327_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR6016905.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29727..30581)
                     /gene="ampE"
                     /locus_tag="OO327_000224"
     CDS             complement(29727..30581)
                     /gene="ampE"
                     /locus_tag="OO327_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR6016906.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30578..31129)
                     /gene="ampD"
                     /locus_tag="OO327_000225"
     CDS             complement(30578..31129)
                     /gene="ampD"
                     /locus_tag="OO327_000225"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR6016907.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31217..32110
                     /gene="nadC"
                     /locus_tag="OO327_000226"
     CDS             31217..32110
                     /gene="nadC"
                     /locus_tag="OO327_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR6016908.1"
                     /translation="MPPLRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIVSGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32313..32753
                     /gene="ppdD"
                     /locus_tag="OO327_000227"
     CDS             32313..32753
                     /gene="ppdD"
                     /locus_tag="OO327_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR6016909.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32763..33910
                     /gene="tadA"
                     /locus_tag="OO327_000228"
                     /pseudo
     CDS             32763..33910
                     /gene="tadA"
                     /locus_tag="OO327_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            33900..35102
                     /gene="hofC"
                     /locus_tag="OO327_000229"
     CDS             33900..35102
                     /gene="hofC"
                     /locus_tag="OO327_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCR6016910.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35137..36180)
                     /gene="guaC"
                     /locus_tag="OO327_000230"
     CDS             complement(35137..36180)
                     /gene="guaC"
                     /locus_tag="OO327_000230"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR6016911.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36342..36380)
                     /locus_tag="OO327_000231"
     CDS             complement(36342..36380)
                     /locus_tag="OO327_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6016912.1"
                     /translation="MIIKTLKMSARK"
     gene            36405..37025
                     /gene="coaE"
                     /locus_tag="OO327_000232"
     CDS             36405..37025
                     /gene="coaE"
                     /locus_tag="OO327_000232"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR6016913.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37025..37768
                     /gene="zapD"
                     /locus_tag="OO327_000233"
     CDS             37025..37768
                     /gene="zapD"
                     /locus_tag="OO327_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR6016914.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37778..37975
                     /gene="yacG"
                     /locus_tag="OO327_000234"
     CDS             37778..37975
                     /gene="yacG"
                     /locus_tag="OO327_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR6016915.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38075..38464)
                     /gene="mutT"
                     /locus_tag="OO327_000235"
     CDS             complement(38075..38464)
                     /gene="mutT"
                     /locus_tag="OO327_000235"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR6016916.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGGPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38524..41229)
                     /gene="secA"
                     /locus_tag="OO327_000236"
     CDS             complement(38524..41229)
                     /gene="secA"
                     /locus_tag="OO327_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR6016917.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41291..41803)
                     /gene="secM"
                     /locus_tag="OO327_000237"
     CDS             complement(41291..41803)
                     /gene="secM"
                     /locus_tag="OO327_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR6016918.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42034..42951)
                     /gene="lpxC"
                     /locus_tag="OO327_000238"
     CDS             complement(42034..42951)
                     /gene="lpxC"
                     /locus_tag="OO327_000238"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR6016919.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43052..44203)
                     /gene="ftsZ"
                     /locus_tag="OO327_000239"
     CDS             complement(43052..44203)
                     /gene="ftsZ"
                     /locus_tag="OO327_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR6016920.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44264..45526)
                     /gene="ftsA"
                     /locus_tag="OO327_000240"
     CDS             complement(44264..45526)
                     /gene="ftsA"
                     /locus_tag="OO327_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR6016921.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45523..46353)
                     /gene="ftsQ"
                     /locus_tag="OO327_000241"
     CDS             complement(45523..46353)
                     /gene="ftsQ"
                     /locus_tag="OO327_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR6016922.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46355..47275)
                     /gene="ddlB"
                     /locus_tag="OO327_000242"
     CDS             complement(46355..47275)
                     /gene="ddlB"
                     /locus_tag="OO327_000242"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6016923.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47268..48743)
                     /gene="murC"
                     /locus_tag="OO327_000243"
     CDS             complement(47268..48743)
                     /gene="murC"
                     /locus_tag="OO327_000243"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR6016924.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48797..49864)
                     /gene="murG"
                     /locus_tag="OO327_000244"
     CDS             complement(48797..49864)
                     /gene="murG"
                     /locus_tag="OO327_000244"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR6016925.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49861..51105)
                     /gene="ftsW"
                     /locus_tag="OO327_000245"
     CDS             complement(49861..51105)
                     /gene="ftsW"
                     /locus_tag="OO327_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR6016926.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51105..52421)
                     /gene="murD"
                     /locus_tag="OO327_000246"
     CDS             complement(51105..52421)
                     /gene="murD"
                     /locus_tag="OO327_000246"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR6016927.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52424..53506)
                     /gene="mraY"
                     /locus_tag="OO327_000247"
     CDS             complement(52424..53506)
                     /gene="mraY"
                     /locus_tag="OO327_000247"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR6016928.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53500..54858)
                     /gene="murF"
                     /locus_tag="OO327_000248"
     CDS             complement(53500..54858)
                     /gene="murF"
                     /locus_tag="OO327_000248"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR6016929.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54855..56342)
                     /gene="murE"
                     /locus_tag="OO327_000249"
     CDS             complement(54855..56342)
                     /gene="murE"
                     /locus_tag="OO327_000249"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR6016930.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56329..58095)
                     /gene="ftsI"
                     /locus_tag="OO327_000250"
     CDS             complement(56329..58095)
                     /gene="ftsI"
                     /locus_tag="OO327_000250"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR6016931.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58111..58476)
                     /gene="ftsL"
                     /locus_tag="OO327_000251"
     CDS             complement(58111..58476)
                     /gene="ftsL"
                     /locus_tag="OO327_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR6016932.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58473..59414)
                     /gene="rsmH"
                     /locus_tag="OO327_000252"
     CDS             complement(58473..59414)
                     /gene="rsmH"
                     /locus_tag="OO327_000252"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR6016933.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59416..59874)
                     /gene="mraZ"
                     /locus_tag="OO327_000253"
     CDS             complement(59416..59874)
                     /gene="mraZ"
                     /locus_tag="OO327_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR6016934.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14470 a        16732 c        14845 g        14366 t
ORIGIN      
        1 tggtactgaa aacgggcaac tccttggtaa tactctcaca ggtaatgatg cagcaaaagg
       61 agtcggtgta ctcatagaag gtttagcaac tagtaaaaat cctctaatga cattgaaacc
      121 taatgattca aattctgttt ataaagatta cgacccgaga ggcaaagacg atacaacagg
      181 aggagtttac ccggatcaag atacaggtat aacataccct ctccatttcc aggccacgct
      241 acaacaggat ggaactatac caatagaagc tggtgagttt aaagccacca gtactttcca
      301 ggtaacctac ccttaataag tccaccgcac cgccatcatc tggcggtgcg gtaattgata
      361 aatcattgcc cccaccaatg tgactgataa aatgccaggc aacacgacat catttatcag
      421 gatacgttat gaaaccgacc accattgctt cacttcagaa atataagcag gataaaaagc
      481 gtttcgcgac catcaccgct tacgactaca gcttcgcgaa actatttgct gatgaagggc
      541 ttaacgtcat gctggtaggc gactcgctgg gcatgaccgt tcagggccac gattccactc
      601 tgccagtaac cgttgctgat atcgcctacc acactgccgc cgtacgtcgc ggcgcaccaa
      661 actgcctgct attggctgac ctgccgttta tggcgtatgc cacgccggaa caagcctttg
      721 aaaacgccgc aacggttatg cgtgccggtg ccaatatggt caaaattgag ggcggtgagt
      781 ggctggtcga aaccgtaaaa atgctgaccg aacgtgccgt tcctgtatgt ggtcacttag
      841 gtttaacccc acagtcagtg aatattttcg gtggctacaa agtacagggg cgcggcaatg
      901 aagcgagcga tcgactactc agcgatgcat tagccttaga agccgcgggg gcacagctgc
      961 tggtgctgga atgcgtgccg gttgagctgg caaaacgtat taccgaagca ctggcgatcc
     1021 cggttattgg tattggtgca ggcaacgtca ctgacgggca aatcctcgtg atgcacgacg
     1081 cctttggtat taccggcggt cacattccta aattcgctaa aaatttcctc gccgaaacgg
     1141 gcgacatccg cgcggctgtg cggcagtata tggctgaagt ggagtccggc gtttatccgg
     1201 gcgaagaaca cagtttccat taaggagtca cgttgtgtta attatcgaaa ccctgccgct
     1261 gctgcgtcag caaattcgcc gcctgcgtat ggaaggcaag cgcgtggcgc tggtgcctac
     1321 tatgggtaat ctgcacaatg gccatatgaa gctggtcgac gaagccaaag cccgcgccga
     1381 tgtggtcgtc gtcagtattt tcgttaaccc gatgcagttc gaccgcccgg aagatctggc
     1441 acgttatcca cggaccttgc aggaggactg cgagaagcta aacaaacgta aagtggattt
     1501 agttttcgcc ccttcggtaa aagagatcta cccgaacggt actgaaaccc acacttacgt
     1561 tgacgttcct ggcctttcga ccatgctgga aggtgccagc cgtccgggac attttcgcgg
     1621 cgtttcgact attgtcagca agctgttcaa cctggtccag ccggacatcg cctgcttcgg
     1681 tgaaaaagat tttcagcaac tggcgttgat ccgcaaaatg gttgccgata tgggcttcga
     1741 tattgagatt gtcggtgtgc caattatgcg cgccaaagac ggtctggcgc taagttcccg
     1801 taacggttat ctgacggcgg aacaacgcaa aattgcgcct ggtctgtaca aagttttaag
     1861 ttcgattgct gacaaattgc aggctgggga acgggatctc gatgaaatta ttaccattgc
     1921 ggggcaagaa ctgaatgaaa aaggcttccg cgccgatgat attcagattc gcgatgccga
     1981 cacattgctg gaagtttctg aaaccagcaa acgggcagta attctggtag ccgcctggct
     2041 tggcgatgct cgcctgatcg acaacaaaat ggtcgagctg gcgtaatact taactggcgc
     2101 tacggctgat ggcgccagtt attaatttac cccacgtcat ctgtcataac acaggtaact
     2161 taaataaggt taattacctt atcaatctca gcaagaggta aatttgccat ctctgcaacg
     2221 tcttcccgag acattccttt acttagaaga cgcagggcga attcttgcct ttcctcctgt
     2281 cttccctgct gaatcccctt ctcaatccct ttctcttcaa accactgcgc cagcgtcatc
     2341 atagacttcc ctcccgtttc cctgtctctc agcacaccat aaaacaaatc cgcttgttca
     2401 gtatgaccgc gttgcagcat atagttttgc atggcaacta actgacttcc gctagtgtac
     2461 ccttcgtcaa tcagcgtgac cagttgctcc agcaataaca ttaagtcgcg ctggcgaata
     2521 tgtttttgca gtagttcgag aatcgcaatc cgccgatgtt gcatgatttc gtcatccggt
     2581 gtgatggtga tatccaccag cgggaaagga ctgttataga cgcgtcgcgc cagctccggc
     2641 gagtaaaaca tatcaaacca gcacattgag agcggataag gtgtggcctc gccctgataa
     2701 aacagtatcg gcaccaccag cggcagctta tcgtggccag cctccagatg ccggtgcatg
     2761 gcagcaatag aataacgcat catgcgaaag gccattttct tatccggctt gctttggtgt
     2821 tcaatcacaa catgcagata accgggattg ccctgcattt gcatggaata gagcacgtcc
     2881 gtgctgtgac ctttcaggca ctcttcaatg aaactccccg actctaaatg aagcgtgttg
     2941 agatcacaaa gttcgcgtaa ttccactggc aaatgtatct ccagaaagtc gcgagccgtc
     3001 tccgcatgca ttaaaaattg tttaaatacc gcgtcatgcg gtgtggtact cggtgcatcc
     3061 atcttccctg tcttccgtgt ggccttgcgt gaaggcagta tccagtcaag gcgtgctatc
     3121 gtcagccacc tctttattgt ttaccgagca gcgttcataa aaatatcaat tcgttacagg
     3181 cgttacatgg cacgcttcgg cgcggtacgc aaactgctga cgcaaatgcc atcagatgag
     3241 tagacactca tcaaaaatcg ggcaatactg cgtgagaatt tcctcaaagc aggcaccgcc
     3301 tgcttcgtta acgacagggt agaaaggtag aagttatgat tcgcacgatg ctgcagggca
     3361 aactccaccg cgtgaaagtg actcatgcgg acctgcacta tgaaggttct tgcgccattg
     3421 accaggattt tcttgacgca gccggtattc tcgaaaacga agccatcgat atctggaatg
     3481 tcaccaacgg caagcgtttc tccacttatg ccatcgcggc agaacgcggt tcgagaatta
     3541 tttctgttaa cggtgcggcg gcccactgcg ccagcgtcgg cgatattgtc atcatcgcca
     3601 gcttcgttac catgccagat gaagaagctc gcacctggcg acccaatgtc gcctattttg
     3661 aaggcgacaa tgaaatgaaa cgtaccgcga aagcgattcc ggtacaggtt gcttgattgt
     3721 tatccctgcg gctggttact caccagccgc gacatcttct ccagcgaatc cgttcttaag
     3781 atataaagtc gttttagtaa caacggatta tcccccggtt ttactttgcc tttcatggtt
     3841 gtcaccgcca ggtgaaatcc ggcatcgttt gctgccttca cggctttgtc attaaagccg
     3901 ccaaacggat acgaaagata ccagacatgt ggattaaatt gcgccagagc gcggcgtgaa
     3961 cgtgcaaaat caaacagaat attgtgctca ctacggctca gtaatatggg tcggcgataa
     4021 ccatctaccc gatgcaaaaa atgggtatgt gactggaaat caaaaacatc gcgaatttcg
     4081 ttaagctcag aaacgctcat aaattgcagc gattttgggt tccacttctg cgggtgacgt
     4141 ttgatgcgtg aggtaacaat aaacaccgtc gccttcatgc catattgttt caacacagga
     4201 tacgcatagc ggctcaccga cttgaggcca tcatcaaagg taatcaccac cgctcgcgca
     4261 gggagattga tcgtattctt cacgtaacct tccagctgag ccatgctcag tgtcgcgtat
     4321 cccctgtcgc gcagccaggc catttggtta ttgaaagcgc gtaccgaggt ggtcgtcgaa
     4381 gtatggcgaa aacgggtgtt ttcttcgtcg cgcagaatat ggtgataagt tagcaccggc
     4441 aggccattat cgggttgggc atccagcgcg ctgatatagg ccagtcgatc gccaatacgg
     4501 atctgatacc aggtttgatt taacctgtct ttcagtttat gcaaaatcgg gtagcgcaaa
     4561 ttgtccgcca gtaccccaaa tggcgcactg cccacactcg gcgcgttgta gaccggcgta
     4621 tctttccagg taattaagtt ctgattactc agcggcttgt tgaggtcacc caaaccgtct
     4681 tcaacttttt gtcgtccctg aactggctcg agatgccctt tatcgataaa ccctttacca
     4741 aagccaaaat taaatgcgta ataacttgcg gcagtgggct ccacggcaat gatttgtccg
     4801 gcccgaatat tccccacggt caccatcttg tcactaattt gcgcccagac cgcagcattt
     4861 tcgatggttt gcatataacg ggcaggtaac gcggcactga cacttgcggt aaacagcatc
     4921 agcaggagaa taacagcttg tttatacatg aaaaaataac cgagggtgaa acaacgcttt
     4981 tattgtagca aaagcgccat cagaatcgcg ggttaatact catacaaatt atgtttgagg
     5041 acgaaaggcg gatttttttg ttgttggtgc caaagactac tcacctccgg cccacccagt
     5101 tcgacaatac actcacgaaa ctctgaactg gtcatggttt cacggcaagc catcatctgt
     5161 tcaattaacg gtaacgtgac accagaaatc acttcgcaac gggagtgttt gtggcttaat
     5221 aatgaagcca cgcgatacgg tggcgctccg gctatatcgg ttaagaagat gacaccctca
     5281 ccagagtccg cttcatgcag agcatcgcac atcatgcggc tgagcatatt agagcttaat
     5341 ccgcgccaga aattcacggc ccggcactga agaagtgccc catgtttttt ctccagcgca
     5401 tccagtatct cttgcgcccg gtcatcgtga caggtaatta cccaacctaa catcgccttc
     5461 ctccttagcc gaaaatcagc ctaccctgac cattgttgat aaagggtgat agccgtcaaa
     5521 actcctctcc cccgaatcca gaggagagga aatagcctta gctacgcaaa ccacgtccac
     5581 gttggatcag cgaccaacag atcaaataaa acgccacaat aaagaccacc agtacgccaa
     5641 aggtagtgac cagcggcaca tcatttatac cgaggaagcc gtagcggaaa ccactgatca
     5701 tataaacgat tgggttcagg tgcgacagcc cttgccagaa cggcggcaac aaagtcagtg
     5761 agtaaaagac cccgcccaaa tacgtgagtg gcgttaacac aaaggttggc accaggctga
     5821 tgtcatcgaa cgttttggca aacacgccgt tcagcaaacc cgcaagggag aatagcaccg
     5881 ccgtgagcac cagggttaag gcaacgaata cccacgaatg cacctgaaac ggcacaaaaa
     5941 acagtgaaat tgccgtcaca agaatgccaa caaacagacc acgcgccacg ccaccgccga
     6001 catatccggc aataatgacg tgagtcggaa ccggcgctac cagcagctct tcaatattac
     6061 gctggaactt ggcaccaaaa aatgatgacg cgacgttagc gtaggcgttg gtgatcaccg
     6121 acatcatgat cagccccggt acgatgaact gcatatagct gaagccatgc atatcgccaa
     6181 tacgcgaacc tatcaggtta ccgaagataa taaagtaaag ggtcatggtg atgactggcg
     6241 gcaccagcgt ctgcacccag atacgcataa agcgatggat ctctttcgcc cagatgcttt
     6301 ttagcgccac ccagtaaaga tgcatcatgc gcgatctcct tgtttttcat taaccagtga
     6361 aacaaacagc tcttccagac ggttagcttt gttacgcata cttaatacct gaatgccctg
     6421 ctcacttaac tgcgtaaata cgctgttgat cccctgctca cgtagcactt caacttccag
     6481 cgtcgcggta tcgaccagtc gatactgata gccatcgagc ttcggtaacg gacttttcgg
     6541 tgcgagatcg agaataaagg tttccgattt cagcttcgcc agcagcgcct tcatcgaggt
     6601 attttccacc agctcaccat gttgaataat gccgatattg cggcacagca tttctgcttc
     6661 ttccaggtag tgtgtggtaa gaatgatggt ggtgcctttg tcgtttaaat ccttcaaaaa
     6721 gccccacatt gagcggcgaa gttcaatatc cacgcctgcg gtcggttcgt cgagaatcag
     6781 tagtttaggt tcatgcatta acgcacgggc aatcattaaa cggcgcttca tcccgccaga
     6841 taacatacgc gcacgttcgt tgcgttttcc ccataaatcg agctgcttaa gatatttttc
     6901 gctgcggatg tacgcctctt tgcgctccac accgtagtac cctgcctgat tcaccacaat
     6961 ttgctgcacg gtttcaaacg ggttgaagtt aaattcctgc ggcaccagtc ccaactgacg
     7021 tttagcgttc acgacatctt tctcgagatc gtaaccaaat acgctgaccc gcccggaggt
     7081 tttatttacc agagagctga taataccgat agtggtcgat ttcccggccc cgttcggccc
     7141 gagaagcgca taaaaatcac ccgcttcgac ctgcaaatct attccacgaa gcgcctgaac
     7201 gccgcctgga tagttttttt taagctgttg aagttccagt gcaatggtca taaattttta
     7261 cttaccttac gttcttacac tttatctgtg gtttaaatcg tcccggagtt gccctatatt
     7321 agccaaacgt aattatttgg ttacaggtcg ttaacctcca tgaaagacat agatacactc
     7381 atcagcaaca atgcactatg gtcaaaaatg ctggtggaag aggatcccgg gttttttgag
     7441 aaactggcac aagcgcaaaa accgcgcttt ctatggattg gatgttccga cagtcgcgtt
     7501 cctgcagaac gtttaaccgg tcttgagccg ggcgaactct ttgttcaccg taatgttgct
     7561 aacctagtca ttcacaccga cctgaactgc ctttccgtgg ttcagtatgc agtggatgta
     7621 ctcgaagttg aacacattat tatctgtggc cactacggtt gcggcggcgt acaagccgcc
     7681 gttgaaaacc cggaactggg gcttatcaac aactggctgc tgcatatccg cgatatctgg
     7741 ttcaaacata gctcattgct cggcgaaatg ccgcaagagc gccgtctgga taccttgtgt
     7801 gaactgaacg tcatggaaca ggtgtataac ctgggccact ccaccattat gcaatcagcg
     7861 tggaaacgcg ggcagaaagt taccattcac ggctgggcct acggcattca cgacggcttg
     7921 ctgcgtgatc tggatgttac cgccaccaac cgcgaaaccc ttgagcaacg ttaccgtcac
     7981 gggatttcca acctcaagct gaaacacgcc aaccacaaat aaaaatgcca tgccggatgc
     8041 aacacatccg gcaacttcac acttactcgt ccagcagaat cactttgcca atatacggca
     8101 gatgacggta acgctgtgcg taatcaatgc cgtaacccac cacaaactca tccgggatcg
     8161 agaaaccgat aaattcaacc gggacgttca cttcacgacg ggacggttta tccagcagcg
     8221 tacaaatcgc caacgacttc ggttcgcgca ggcttaagat ctcacgcact ttcgacagtg
     8281 tattccccga gtcgatgata tcttcaacaa tcagcacgtc cttgccacgg atatcttcat
     8341 ccagatcttt gaggattttc acatcacggg tggtggacat gccgctaccg tagctggagg
     8401 cggtcataaa gtcgacttca tgagatacct gaacttcacg gcacaggtcc gccataaaca
     8461 taaatgagcc acgcagcaga cccaccagca ccatatcgct gccgctgtct ttgtaacgct
     8521 cagtaatctg acgacccagt tcggcgatac gcgctttaat ctccgcttcg gggatcatta
     8581 cttctacagt atgtttcata tctctaacca tatgatttaa aaacaaatca cttcgcacgg
     8641 gcaggttttg gcaccgcaac aaagcgcaag acgcgcagta taccagtaaa actattcatt
     8701 cgcggaatct gttaataaag ctcgaattgt gatgacggtc acacatgtta aaacctataa
     8761 ttaattgcta ctaaaatatt aatgaattga aatggtgtct ctttatggca attaacaata
     8821 cagcctcgcg acgattactc gtcacgctaa cagccctttt tgcagcgctt tgcgggctgt
     8881 atctactcat tggcggaggc tggctggtcg cgattggcgg ctcctggtac taccctatcg
     8941 ctggccttgt gatgctcggc gtcgcctgga tgctgtggcg cagtaaacgc gccgcgcttt
     9001 ggctatacgc agccctgctg ctcggcacca tgatttgggg cgtctgggaa gttggtttcg
     9061 acttctgggc gctgactccg cgcagcgata ttctggtctt cttcggcatc tggctgatcc
     9121 tgccgtttgt ctggcgtcgc ctggtcattc ctgccagcgg cgcagttgcc gcactggtgg
     9181 tcgcactgct gattagcggt ggtatcctga cctgggccgg atttaacgat ccgcaggaga
     9241 tcaacggcac cttaagcgcc gatgccacac ctgccgaagc tatctccccc gttgccgatc
     9301 aggactggcc tgcgtatggc cgcaatcagg aaggtcaacg cttttcacca ctgaagcaaa
     9361 ttcacgccga taacgtccac aagctgaaag aagcctgggt gttccgtact ggcgatgtga
     9421 agcagccgaa cgatccgggt gaaatcacca atgaagtgac gccgattaaa gtgggcgata
     9481 ccctttacct gtgtaccgct caccagcgcc tgtttgcgct tgatgccgcc agcggcaaag
     9541 agaaatggca ttacgatcct gagctgaaaa ccaacgagtc tttccagcac gtaacctgcc
     9601 gtggcgtctc ttatcatgaa gccaaagcag aaacggcttc gccggaagtg atggcggatt
     9661 gcccgcgtcg tatcattctt ccagtgaatg atggtcgcct gattgcgatt aacgctgaaa
     9721 acggcaaact gtgcgaaacc ttcgccaata aaggcgtgct caatctgcaa agcaatatgc
     9781 cagacaccaa accgggtctg tatgagccga cttctccgcc aattatcacc gataaaacca
     9841 tcgtgatggc cggttcagtc accgataact tctcaacccg cgaaacgtct ggcgtgatcc
     9901 gtggttttga tgtcaacacc ggggagctgc tgtgggcttt tgatccgggc gcgaaagatc
     9961 cgaacgcaat cccgtcagac gaacacacct ttacctttaa ctcaccaaac tcgtgggcac
    10021 cagcggccta tgacgcgaag ctggatctgg tctatctgcc gatgggcgtg accacgccgg
    10081 atatctgggg cggtaaccgc acaccggaac aggaacgtta tgccagctcg attctggcgc
    10141 tgaatgccac taccgggaaa ctggcgtgga gctaccagac cgttcaccac gacctgtggg
    10201 acatggatct tccggcacag ccgacgctgg cggacatcac cgttaatggt cagaaagtgc
    10261 cgattattta cgctccggcg aaaaccggta acatttttgt gctcgatcgt cgtaatggcg
    10321 aactggtggt tccggccccg gaaaaaccgg ttccccaagg tgctgccaaa ggcgattacg
    10381 ttacccctac tcaaccattc tcggaactga gcttccgtcc gacgaaagat ttgagcggtg
    10441 cggatatgtg gggagccacc atgtttgacc aactggtgtg ccgcgtgatg ttccaccaga
    10501 tgcgctatga aggcattttc acgccgccat ctgaacaggg tacgctggtc ttcccgggta
    10561 acctggggat gttcgaatgg ggcgggattt ccgttgatcc aaatcgtgaa gtggcaattg
    10621 ccaacccaat ggcactgccg tttgtttcga aactgctccc gcgtggtcct ggcaacccga
    10681 tggagcagcc gaaagatgcc aaaggcacgg gtacggaatc cggcattcag ccacagtacg
    10741 gtgtaccgta tggtgtcacg ctcaacccgt tcctctcacc atttggtctg ccatgtaaac
    10801 agccagcatg gggttatatc tcggcgctgg atctgaaaac taatgaagtg gtgtggaaga
    10861 aacgtattgg tacgccgcag gacagtatgc cgttcccgat gccggttccg gtgccgttca
    10921 atatgggtat gccgatgctg ggcgggccaa tctccacggc gggtaacgtg ctgtttatcg
    10981 ccgctacggc agataactac ctgcgcgctt acaacatgag caacggtgaa aaactgtggc
    11041 agggtcgttt accggcgggt ggtcaggcaa caccgatgac ctatgaagtg aatggcaagc
    11101 agtacgtggt gatctccgca ggcggtcacg gttcatttgg tacgaagatg ggcgactata
    11161 ttgtggctta tgccctgccg gatgatgtga aataaccata atgcccggag agatccgggc
    11221 atatttccga atacggtctt tttataccgt aaaccctaac atcatccccg tatcttcatg
    11281 ctccagcaga tggcagtgcg ccatataagc atgttctttc ggtgcgttgt gattaaactt
    11341 caccagcact tcactgacat tgccttctac cttaacggta tctttccagc ccgcgcgatg
    11401 agccgctggc ggtttgccat tttctgacaa gatacggaac tgcgtgccgt gaatatggaa
    11461 cggatgcagc atcatgtcgc ccacgccaga gataacccaa cgttcgtact gccctttcgc
    11521 cgccgcaaac atcggcttat tcatatcaaa cgcctgaccg ttgattttat tggcatggtg
    11581 gaaatcgaac ttcccgccgt ggttcatatg attcatattg ccgtgcccca tatggcccat
    11641 catctggctg tgatccattc cggccatcgc ctgatcgcca tatttctcca ttagcatctg
    11701 catccccatc atatcgagca tcgggtccat agaaagttgc agcttgcgta ccgtcagccc
    11761 ttccagcgaa ggtaacgcag gcaggctact taatgtgtca ggcaaagcac cggaggcact
    11821 aatagcaatc ggctgaatcc gcattaccgg atgcggcttg tcaaacggtg caatcgccat
    11881 ccccatctgg ctgaccggca gcgtcaccag gtcaaagggt ttgttgtcgt taacctccac
    11941 cagcacttca aaacgctcgc ccatcagcac cggcagctcg ctcaccttca ccggttcagg
    12001 tagcagacca ccgtcgctgg caatcacata cagcgggcga ttgtcgctgg tggcgaaatt
    12061 gagcgagcgg gcattacagc cattgagcaa acgcaggcgc agccaaccac gcggggcagc
    12121 gtgttgtggg tagattgcac cgttggtcag cagcgtatcg ccaaaccagc ccacggcggc
    12181 ggtcatcaca tccagttgat aatcaatctg cccgtcggcg ctaaatttct tatcctgaac
    12241 gatcaccgga acatcatcga taccccactg ttttggcagc attaatttca ggatctcgtc
    12301 atcttcaatc accaccagac cagccagccc catcgccacc tgtcgcccgg tcttgccatg
    12361 ttgatgtgga tggaaccagc aggtagcggc aggttgatca acgttcaacg tcaccgagcg
    12421 cttgccacct ggcggaataa ttccctgtgg gccgccgtcg acgtcacccg gtacttccag
    12481 cccgtgccag tgcaacgtcg tctcttccgc cagttggttg tagatatcaa ccgttaccgc
    12541 tttgccgcgc tgtaatttca ccgccggccc cagcagattg ccgttatagc cccaggtagt
    12601 tgcagttttc tcgccaaagg tggactgacc tgcgccaata gttaactgaa tgcgattacg
    12661 ggcatcggtc gtgagcaaat cagggatcgg taacgttggg cgttccgccg caaagactgc
    12721 acggctccac agtggcaagg ctgaagccac acccagtgcg acggaatatt tcaagaaatc
    12781 acgacgttgc atagttattt ccttattctt aagcaggcga aacaaaatca aacgttgagc
    12841 atagtccttc cccttacggg aaggtcaagc cgcagacaca atttaatcga caataatggt
    12901 cgtcgcctcg ctcgcagtgt gctaacgttt atcttcttta agcccctggt agaagcaatg
    12961 aagacgtttt tcagaacagt gttattcggc agcctgatcg ccgtttgcgc aaacagttac
    13021 gcgctcagcg agtctgaagc cgaagatatg gccgatttaa cggcagtttt tgtctttctg
    13081 aagaacgatt gtggttacca gaacttatct aacgggcaaa ttcgtcgcgc actggtcttt
    13141 ttcgcccagc aaaaccagtg ggatctcagt aattacgaca ccttcgacat gaaagctctc
    13201 ggtgaagaca gctaccgcga tctcagcggc attggcattc ccgtcgctaa aaaatgcaaa
    13261 gccctggccc gcgattcctt aagcctgctt gcctacgtca aataatccct gatacctttt
    13321 tgtagaaata ttgaccgtgc atccgcggtc aatgttagct attatgttgc gccctttttt
    13381 tacgggtgtt aacaaaggag gtatcaaccc atggccgaaa aaaaacagtg gcatgaaacg
    13441 ctacacgacc agtttgggca gtactttgcg gtagataacg ttctgtatca tgaaaagacc
    13501 gatcaccagg atctgatcat ttttgagaac gctgcatttg gtcgcgtaat ggcgctggat
    13561 ggcgtagtac aaaccaccga gcgcgacgag tttatctatc atgagatgat gacccatgtt
    13621 ccgctactgg cccacggtca cgcgaaacat gtgctgatta tcggcggcgg cgacggtgcc
    13681 atgctgcgtg aagtaacccg acataaaaac gttgagtcaa tcacgatggt ggaaatcgat
    13741 gcgggtgtcg tgtcgttctg ccgtcagtat ctgcccaacc ataacgccgg tagctacgac
    13801 gatccgcgct ttcaactagt gatcgacgat ggcgtcaatt tcgttaatca aaccagccag
    13861 acctttgatg tcattatctc cgactgcacc gatcctatcg gtcccggcga aagcctgttc
    13921 acctcagcat tttatgaagg ctgcaaacgt tgcctgaatc ctggcggtat cttcgtcgca
    13981 caaaacggcg tctgcttttt acagcaggaa gaagccatcg acagccatcg caaactcagc
    14041 cattacttca gcgacgttgg cttttatcag gcggcgatcc cgacctatta cggcggtatc
    14101 atgacttttg catgggcgac agataacgac gccttacgcc atctctcaac cgaaattatt
    14161 caggcgcgtt ttctcgcttc tggcctgaaa tgccgttatt acaatccggc aatccatacg
    14221 gcagcttttg ccttacctca gtatctgcaa gacgcactgg cttcacagcc gtcctaagga
    14281 gaagataaga aattgaaaaa actgaaactg catggcttta ataatctgac caaaagtctg
    14341 agtttttgta tttacgatat ctgctacgcc aaaactgccg aagagcgcga cggttatatt
    14401 gcttatatcg atgaactcta taatgccaac cgtctgaccg aaatcctgtc agaaacctgt
    14461 tccattatcg gggccaatat tcttaacatc gcccgccagg attacgaacc acagggtgcc
    14521 agcgtcacta ttctggtgag tgaagaaccg gttgacccga aactcatcga caaaacagaa
    14581 caccccggcc cactgccaga aacggtggtt gctcatctcg ataaaagtca tatttgcgta
    14641 catacctacc cggaaagtca tcctgaaggc ggtttatgta ccttccgcgc cgatattgaa
    14701 gtctctacct gcggcgtgat ttctccgctg aaggcgctga attacctgat ccaccagctt
    14761 gagtccgata tcgtaaccat tgattatcgc gtgcgcggtt ttactcgcga cattaacggt
    14821 atgaagcact ttatcgacca tgagattaat tcgattcaga actttatgtc tgacgatatg
    14881 aaggcgctgt atgacatggt ggatgtaaac gtctatcagg aaaatatctt ccataccaag
    14941 atgttgctta aagagttcga ccttaagcac tacatgttcc acaccaaacc ggaagattta
    15001 accgacagcg agcgccagga aattaccgct gcgctgtgga aagaaatgcg cgagatttat
    15061 tacgggcgca atatgccagc tgtttaacgg ctctggcgga gctcccaggc tccgccagat
    15121 ttatttactt ctgctgcacg aaattgcggt aagccgccac gacctgcaga aaatcctcaa
    15181 cgccgcatag cgacaggctt tcttcgtcgt agtagttcat cccctcttcc atttcatcgc
    15241 cagcgaattc cagttgattg gcgcgaacca tcacctcttc agcgtccatc cacagggtgt
    15301 attcatgccc tgcccgttgc caggaccgtt cgctaccttt cagtgcgtgc gctgcttgtt
    15361 ccacttcatc aagcaaggcc aggttttctt tcacctcttc attaaaccag tgcccgacca
    15421 cttcatgccc catggacata cgcaccttta ccactccggt aatatcgcgc agaaattcgt
    15481 aatccatagt gtgttcctct gctcccggca atgcgccgaa atcgcatcgc gccattgatg
    15541 taattatcgc agccttaccg gagaaaaaca gcggaacggc gatagtttag aaataaaaaa
    15601 tgcccgggaa tctcattcac cgggcattgt gtcgtttatg cgcagcgcgt gcgcttactt
    15661 attacaccgc agtctggaaa atcaccccat cggctttttc ggtgtactga gaaagctggt
    15721 tgaagttcag ataacgataa gtatcaacgg cagttttatc tacttgcgca acgtaggtct
    15781 ggtactcttc cggcgtcggc agtttgccaa tcagcgccgc aacagccgcc agttctgcag
    15841 aagccaggaa gacattcgcg ccagtaccca aacggttcgg gaagttacgg gtagaggtgg
    15901 aaaccaccgt cgcaccgtct gctacacgcg cctggttacc catacacagg gaacagcccg
    15961 ggatctcgat acgagccccg ctcttaccga agacgctgta gtagccttct tccgtcagtt
    16021 gcgcggcgtc catacgggtt ggcggtgcca cccacaggcg ggtcggcaac tgacctttgt
    16081 gcgcatccag cagtttacca gcggcacgga agtgaccgat gttagtcatg caagaaccga
    16141 taaatacttc gtcgatcttc tcgccctgta ccgcagacag cggacgtgcg tcatccggat
    16201 cgttcggagc acacaggatt ggctctttaa tctccgccag atcgatgtcg atcactgccg
    16261 cgtattccgc atctgcatcg gcttccagca gctcaggatt cgccagccat ttttccatgc
    16321 cctgaatacg gcgttccagg gtacgacgat cgccgtaacc ttccgcgatc atccacttca
    16381 gcaggacgat gttggagctc aggtattcga tgatcggttc tttgttcagc ttgatagtac
    16441 aaccagcggc agaacgctcg gcggacgcat cggttagctc aaaggcctgc tcaactttca
    16501 gatccggcag accttcaatt tccagaatgc gaccagagaa gatgtttttc ttgcctttct
    16561 tctcaacggt cagcagacct tgtttgatcg catacagcgg aatggcgtgt accagatcgc
    16621 gcagggtgat gcccggctgc attttgcctt tgaagcgcac cagaacggat tccggcatat
    16681 ccagcggcat tacgccagtt gcggcagcaa acgccaccag accagaaccc gccgggaaag
    16741 agataccgat cgggaaacgg gtatgggagt caccaccggt accgacggta tccggcagca
    16801 gcatacggtt cagccaggag tgaatgacgc cgtcacccgg acgcagcgac acaccgccac
    16861 ggttcataat gaagtccggc agcgtgtggt gcgtgttcac gtcaactggc ttcggatacg
    16921 ccgcggtgtg gcagaaagac tgcatcacca ggtcagccga gaagcccagg cacgccaggt
    16981 ctttcagttc atcacgggtc atcgggccgg tggtgtcctg agagcctaca gaagtcattt
    17041 tcggttcgca gtacgcgccc ggacgaatgc ctttcacgcc acaggcacgg cctaccattt
    17101 tctgcgccag cgagaagccg cgatcgctct cagcgacatc tttcgcctga cggaacacat
    17161 cactgtgcgg cagaccaagt gcttcacgcg ctttggtggt caggccacgc ccgataatca
    17221 gcggaatacg gccgccagca cgcacttcat caatcagcac gtcggttttc agttcgaagg
    17281 tcgccagcag ttcgcctgtt tcgtggttac gcacttcacc tttgtacggg taaacgtcaa
    17341 tcacgtcgcc catgttcagg ttagagacgt tgacttcgat tggcagtgca cccgcgtctt
    17401 ccatcgtgtt aaagaagatg ggtgcaattt taccgccgag gcacaaacca ccgccgcgtt
    17461 tgttcggcac atgtggaata tcatcgccca taaaccacag aacggagttc gtggcggatt
    17521 tacgcgatga acccgtaccc acaacgtcac cgacgtacgc cagcgggaaa cctttctgtt
    17581 gcagagcttc aatctgtttg atcgggccaa caacacctgg ctggtctggc tcaatacctt
    17641 cacgggcgtt tttcagcatc gccagcgcgt gcagtgggat atccgggcgt gaccacgcat
    17701 ccggtgccgg agaaaggtca tcggtgttgg tttcgccagt gactttaaag acggtaacgg
    17761 tcagtttttc agccagcgcc gggcgattca ggaaccattc ggcatccgcc caggactgca
    17821 ttacctgctt cgcatattcg ttgcctgctt tcgctttctc ttctacgtca tagaagttat
    17881 cgaacatcag cagcgtgtga gaaagtgctt tggcagcaat aggtgccagt ttggcatcat
    17941 ccagcgcgtc gatcagcgga tgaatgttgt aaccaccctg catggtgccc agcagttcga
    18001 tagctttttc cggagtcagc agaggggatt tagcttcgcc tttagcgata gcagccagga
    18061 agcctgcttt tacataggcg gcttcatcga cgcctggggg aacacggttg gttaacagat
    18121 ctaacaggaa ttcttcttcg cccgcgggcg ggtttttcag cagctctaca agcgcggcca
    18181 tttggtttgc atccaggggt ttgggcgcaa tcccctcagc ggcacgctca gctacgtgct
    18241 tacggtattc ttctagcacg acggttctcc tcgctctcat tgtcatagtg cggcaggcga
    18301 ttctcttcac gctcctgtga gacagcagtt tgtagggtaa atgcccagta ccgcaacggg
    18361 cagaatagca ggattttgac gacgtgttaa tctgtttaca aaaaagcaac ataaaaaagt
    18421 ggctgaatcg ttaaggatgg tctagagatt gtttccctcc gtaaaaactt cgttaattcg
    18481 catggtgata atcacctttc tcaacgaatc ccaagattag tcaaaattta aactaccgcc
    18541 tctttatact cggattcaca gcacctgcgg gtggcagttc gccgaccatt gcgatttcct
    18601 tgagatccga attatgaaaa tgactttgcc gtttaaaccc catgtgctgg cgctaatttg
    18661 cagtgccggg ctttgtgctg cctctgccgg gctatatata aaaagccgca cagtggaagc
    18721 gcctgtagaa ccgcaatcga cacaactggc tgtgtctgac gctgccgctg ttacgcttcc
    18781 tgcaacggtt tccgcacctc ccgtaatacc cgcagtcgtc aaatccgcat tcagcactgc
    18841 acaaatagat caatgggtcg cacccgtcgc gctgtatccc gacgccctac tttcgcaggt
    18901 gctgatggca tcaacctatc cgacaaacgt tgctcaagca gtgcaatggt cgcacgataa
    18961 tccacttaaa caaggcgatg ctgctattca ggcggtatct gaccagccgt gggacgccag
    19021 cgttaaatca ctggtggcct ttccacaatt gatggcattg atgggcgaaa acccgcaatg
    19081 ggtgcaaaac ctgggcgatg cttttctggc gcagccgcag gacgtgatgg actcggtaca
    19141 gcgattgcgg caactggcgc aacaaaccgg ctcgctgaag tcatcaaccg aacagaaagt
    19201 tattaccaca acgaagaaag ctgtaccggt aaaacagaca gtcacggcac ccgtcatacc
    19261 atccaatacc gttttaactg ccaaccccgt cattacagag ccggcaacaa ccgtcatttc
    19321 cattgagccc gccaatcctg atgtggtcta tattcccaac tacaacccaa ccgtggttta
    19381 cgggaactgg gccaatactg cgtatccgcc ggtttatctg ccaccaccag ccggagaacc
    19441 gtttgttgac agctttgtgc gcggattcgg ctatagcatg ggcgttgcta ccacgtacgc
    19501 actattcagc agcatcgact gagatgacga cgatcatgac catcatcatc atgacgatga
    19561 tgattatcat caccacgatg gcggtcatcg tgacagtaat ggctggcaac acaacggcga
    19621 caacatcaat atcgacgtca acaatttcaa ccgtatcacc ggtgagcatc ttactgataa
    19681 gaatatggca tggcggcaca atccaaacta ccgtaatggt gtgccctatc atgatcagga
    19741 tatggcaaag cggtttcatc aaaccgatgt caacggcgga atgagtgcca cgcagctacc
    19801 tgctccaaca cgcgacagcc agcgtcaggc ggcagcaagt cagtttcagc aacgaacaca
    19861 cgccgccccc gtcattacac gagataccca acgtcaggca gcggcacagc ggtttaatga
    19921 agctgaacac tatgggagct atgacgactt ccgcgacttc agccgtcgcc aaccactgac
    19981 ccagcagcaa aaggacgccg ctcgtcagcg ttatcagtcg gcctctcctg agcagcgcca
    20041 ggcagtccgc gagaaaatgc agactaaccc gcagatccag cagcgaaggg aggcggcgcg
    20101 ccagcgtatt cagtccgcct cgcctgagca gcgccaggcg gttcgcgaga aaatgcagac
    20161 taacccgcag aaccagcagc gaagagaggc agcgcgtgag cgtattcagt ctgcaactcc
    20221 cgaacagcgc caggtgttta aggaaaaagt acagcagcgc ccactgaacc aacagcaacg
    20281 tgataacgcc cgccagcgta tacaatcggc atcacctgaa caacgtcagg tttttcggga
    20341 gaaagttcag gagagccgcc cacaacgtct aaacgacagt aaccatactg tcaggctgaa
    20401 taacgagcaa cggtcagcag tacgcgaacg tctctctgaa cgtggagcaa gaagactgga
    20461 aaggtaaatt gcagacgtaa aaaaagcggc gtggttagcc gcttttttaa ttgccggatg
    20521 ttccggcaaa cgaaaaatta cttcttcttc gctttcgggt tcggcaggtc ggtaatgcta
    20581 ccttcgaaca cttctgccgc caggcccaca gactcgtgca gagtcgggtg cgcgtggatg
    20641 gtcagtgcga tgtcttcagc atcacaaccc atttcgattg ccaggccgat ttcacccagc
    20701 agctcgccgc cgttggtacc aacaatcgca ccaccgatca cacggtgaga ttctttgtcg
    20761 aaaatcagct tggtcatacc gtctgcgcag tcagaagcga tagcacgacc agaagcagcc
    20821 cacgggaagg tggcggtttc atagctgatg cctttctctt tcgcttcttt ctcagtcaga
    20881 cctacccatg caacttctgg ttcggtatag gcgatggacg ggataacttt cggatcgaag
    20941 tagtgtttct taccggcgat aacttcagcg gcaacgtgac cttcgtgaac acctttgtgt
    21001 gccagcatcg gctgaccgac gatatcgccg atagcaaaga tgtgcggtac gttggtacgc
    21061 agctgtttgt caacgcggat gaaaccacgg tcgtcaactt ccacgccagc tttgcctgcg
    21121 tcgaggtttt taccgttcgg cacacgacca atcgctacca gcacggcgtc gtaacgctgc
    21181 ggttcagctg gtgctttttt gccttccatc gtcacataaa taccgtcttc tttcgcttca
    21241 acggcggtaa ctttggtttc cagcatcagg ttgaatttct tgctgatacg cttggtgaag
    21301 actttaacga tgtctttgtc agctgccggg ataacctggt cgaacatttc aaccacgtca
    21361 atctgtgaac ccagcgcgtg gtaaacggtg cccatttcca gaccgatgat accgccaccc
    21421 attaccagca ggcgttctgg tacttctttc agttccagcg cgtcagtgga gtcccagata
    21481 cgcggatctt catgcggaat aaacggcagt tggatcgggc gagaacccgc tgcaatgatc
    21541 gcattgtcga agttgatcac ggttttgccg ttctcacctt caacttccag ggtgttagcc
    21601 ccggtgaatt tacccagacc gttgaccact ttgactttgc ggcctttcgc cataccagcc
    21661 agaccaccgg tcagctgatt gatcactttc tctttccagg tacgaatctt gtcaatatcg
    21721 gttttcggtt cgccgaagac gataccgtgt tcagccagcg ctttggcttc ttcgataact
    21781 tttgctacgt gcagcagtgc tttagaaggg atacagccga cgttcaggca aacaccgcca
    21841 agggtgttgt aacgttctac gattacggtt tccagaccta aatcagcgca acggaaggca
    21901 gcggagtaac ctgcggggcc tgccccaagt accacgacct gagttttgat ttcagtactc
    21961 atcatgacct ctatatattt atctccggcg gtcatacccg tcgtctttca ggccgcagcg
    22021 gcgttagctt cacgccctca ccccagtcac ttacttatgt aagctcctgg ggattctggc
    22081 gcttgctacc ttgctgcacc cagaaatcca tagggtatgt gaagtaatac cctaaccacc
    22141 accgggtcgt tctatccgtc ggtattttac aaaattgtta acaattttta aacaacaaac
    22201 ggcaaccgat ttgtctattc gctaataacc tcaatacatt catgagatta ccagaaaaaa
    22261 gccggccgtt gggccggctc ttttacttac atcaccagac ggcgaatgtc agacagcgtg
    22321 ttgttaatga tggtaatgaa acgggcacca tcagcaccgt cgatcacgcg gtggtcgaag
    22381 gagagagaaa tcggcagcat cagacgcggc acgaactctt taccattcca caccggctcc
    22441 atcgcggact tggaaacgcc gaggatagcc acttccggcg cgttcacaat cggcgcgaag
    22501 tgggtagtac ccaggccgcc gatgctggag atggtgaagc aaccgccctg catttcgccc
    22561 gcagtcagct taccgtcacg cgctttctta gaaatagtca tcagctcgcg agacagctcg
    22621 atgatgcctt tcttgttgac gtctttgaat accggaacaa ccagaccgtt cggggtatcc
    22681 accgccacac cgatgttgat gtatttcttc agggtcagac gctgaccgtc ttccgacagc
    22741 gaactattga agcgaggcat ctgctcaaga gctgcagcaa cggctttcat gatgaagaca
    22801 accggggtga tcttcacatc cagcttacgt ttcgccgctt cttcgttctg ctgtttacgg
    22861 aacgcttcca gctcggtgat atcggttttg tcgaagtggg taacatgcgg gatcatcacc
    22921 cagttacggc tcaggttagc accagaaatt ttctggatac ggcccagttc cacttcttcg
    22981 atttcgccaa acttgctgaa gtccaccttc ggccacggca gcatgccagg gataccaccg
    23041 ccagtcgccg ccggagctgc ttctgcacgt ttgatagctt ctttcacgta agcctgaacg
    23101 tcttcgcgca ggatacgacc tttacggcca gtgcccttca ctttcgccag gttaacgcca
    23161 aactcgcgtg ccagacggcg gatcagcggg gtcgcgtgaa cgtaagcgtc gttttcagca
    23221 aattcagatt tgccttccgc tttcgcagcc ggagcttcag cttttgctgc cggagccggc
    23281 gctgccgctt cctgtttcgc aggagctgcc gcaggcgctg cgccttcaac ttcgaagatc
    23341 ataatcagcg aaccagtttt cactttatcg ccaacgttga ctttcagttc cttcacgacg
    23401 cctgcaaacg gagccggaac ttccatagaa gctttatcgc cttctacggt aatcagtgac
    23461 tgttcagcgg caactttgtc gcccactttc accatcactt cggtcacttc aacttcgtca
    23521 ccgccgatat ccggaacgtt aacttctttc acgccagctg ctggtgcagg ggtcgctgcc
    23581 ggagtcgctt cctgtttagc cgccggagct gccgcgcctg cttcacccgc gacttcgaag
    23641 accataatca gcgagccggt agacactttg tcacccacgt tcactttgat ctctttcacg
    23701 gtacctgcaa acggagccgg aacttccata gaagccttgt cgccttctac ggtgatcagc
    23761 gactgttcag cttcaacttt atcgccaact ttcaccagga tttcggtcac ttcaacttcg
    23821 tcgctgccga tatccggaac gttaacgtct tttgccgccg cagccgcggg tgctgctgcc
    23881 ggagctgctt ctttcttctc ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg
    23941 gaatcgaaaa tcataatcag tgcgccggtc tgggttttat cgccaacaga gactttgatc
    24001 tctttaacga tacccgcctg cggagatgga acttccatag aggctttgtc gccttctacg
    24061 gtgatcagcg actgttcggc ttcaactttg tcgcccactt tgaccaggat ctcggtgatt
    24121 tcaacttcat cagccccgat gtccggtact ttgatttcga tagccattat tcttttacct
    24181 cttacgccag acgcgggtta actttatctg catcgatgtt gaatttggcg attgcgtcag
    24241 caaccacttt cttatcgatt tcgccacgtt tagccagttc gcccagcgcc gcaaccacga
    24301 cataagaagc atcaacttcg aagtggtgac gcaggttctc acggctgtcg gaacgaccga
    24361 agccatcagt acccagtacg cgatagtcgt cagccggtac gtaagtacgg acctgctcag
    24421 cgaacagttt catatagtcg gtagatgcca ctgccggcgc gtcgttcatc acctgagcga
    24481 tatacggaac gcgcggagtt tccagcggat gcagcatgtt ccagcgttca caatcctgac
    24541 catcacgcgc cagctcggtg aaggaggtca cgctataaac gtcagaacct acgccgtaat
    24601 ctttcgccag gatctctgcc gcttcacgga cgtgacgcag gatggaaccg gagcccagca
    24661 gctgaacttt acctttgcta ccttcaatgg tttcgagttt gtagatacct ttacggatac
    24721 cttcctcagc accttccggc attgccggca tgtggtagtt ttcgttcagc gtagtgatgt
    24781 agtagtaaac gttctcttgt ttttcaccgt acatacgctc cagaccgtca tgcataatga
    24841 cagcaacttc gtaagcgtaa gccgggtcgt aagagataca gttcgggata gtcagcgact
    24901 gaatgtggct gtgaccatct tcgtgctgca gaccttcacc gttcagggtg gtacgaccgg
    24961 aagtaccgcc gatcaggaag ccacgcgctt gctggtctcc agccgcccag cacagatcgc
    25021 cgatacgctg gaagccgaac atcgagtaat agatgtagaa tgggatcatc ggcagattgt
    25081 tcgtgctgta agaggtcgcc gctgccagcc aggaacaccc tgcgcccagc tcgttgatcc
    25141 cttcctgcag aatctgacct ttctcgtctt ctttatagta agcaacctgc tcgcggtcct
    25201 gcggggtgta ctgctgaccg ttcgggctgt aaataccaat ctgacggaac agaccttcca
    25261 taccgaaagt acgcgcttcg tcggcgatga tcggtaccag acgatctttg atcgacttgt
    25321 tcttcagcat cacgttcaga gcacgaacga aagcgatagt ggtagagagc tctttgctct
    25381 gctcttccag cagcgcgccg aagtcttgca ggctcggcag ctcaagcttc tcggtgaagt
    25441 tcggctgacg gcttggcaga taaccgtgca gtttctgacg ctgagcgtgc agataggtat
    25501 gctcttcaga accttccggg aaggtgatgt acggcagttt ttcgatatct gcatcagaca
    25561 ccggcacatt gaaacggtcg cggatgtgac gcacgccgtc catgttcatt ttcttaacct
    25621 ggtgcgcgat gtttttacct tcagccgcgt cgcccatgcc gtaaccttta atggtatgag
    25681 caaggattac tgtcgctttg cctttggttt cctgcgcttt cttgaatgca gcgtagattt
    25741 tcttcggatc gtgaccacca cggttcagtg cccagatctg ctcgtcagtc cagtctgcaa
    25801 ccagtgctgc ggtttcagga tatttaccga agaagtgttc acgaacgtac gcaccatctt
    25861 tcgatttgaa ggtctggtag tcgccgtcaa cggtttcgtt catcagctgg atcagtttac
    25921 cgctggtatc tttacgcagc agttcatccc aacggctacc ccacatcact ttgatcacgt
    25981 tccagccagc accttcgaag atgccttcca gttcgttgat gatcttgccg ttaccggtga
    26041 ccgggccgtc aagacgctgc aggttacagt tgataacgaa aaccaggtta tccagttttt
    26101 cacgggtagc gatggtgatc gcacctttgg attccggttc gtccatttca ccgtcgccga
    26161 ggaacgcgta aacggtctgt ttagaggtat ctttcaggcc acggtgttcc agatatttca
    26221 ggaatttagc ctggtaaata gcaccaatcg gacccagacc catagatacg gtcgggaact
    26281 gccagaattc cggcatcagt ttcgggtgcg gataggaaga gaggccattg ccgtgaactt
    26341 cctgacggaa gttatccagc tgctcctgag tcagacgacc ttccaggaaa gcacgggcgt
    26401 aaacgcccgg ggagatgtgg ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc
    26461 gtgcacggaa gaagtggtta aagcacacat cataaatggt tgcggaagac tggaaggacg
    26521 ccatgtggcc gcccagttcg aggtcttttt tcgacgcacg cagcaccgtc atgatggcgt
    26581 tccagcggat agctgaacga atacggcgtt ccagttccag attacccgga tactccggtt
    26641 gttcttcaac ggggatggtg ttgatgtagt tgctgatacc tgtgcctgcg gctacgttta
    26701 caccgccttt gcgggcttca gcaaacagtt ggtcgatcag atactgagca cgctcaacac
    26761 cttcttcacg gatgaccgat tcgatcgcct ggagccagtc gcgagtttcg atcggatcca
    26821 cgtcatttgg gaaacgttct gacatgggtt attccttatc tatctaataa cgttgagttt
    26881 tctggaacct gtcccattga actctcgcca gaaagctcaa taagacaggt tctacgttta
    26941 gttaccgcgc ttttatatgc gcttgattta caacatcttc tggataattt ttaccagaaa
    27001 aatcactaat tctttcgttg ctccagacga cgcagagaac gctcacggcg gctctcttca
    27061 cggcttctgt cgagcaaaat ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc
    27121 gcttcttccg gcttaccgac cataatcgct tcaaatatgc gggtgcggtg actactcacc
    27181 agcggcagca tctcgcgacg cgaatagagc aattcgaagt tctggcggac attctgggcc
    27241 aacatcggct ccatacacct tagcagatga agcagaacca cattgtgggc cgcttcggtg
    27301 acggcaatct gatactggag tacggcgttt gattccgcgt ccagatcgcc agactgctgc
    27361 gccagctcta tggcgtggtg gagttcacgg atgcgttcct tgtcttcatc ggtactacgc
    27421 agcgcggcgt aataagcggc gataccttcc agggcgtgtc gtgtttcgag caagtcatac
    27481 tgtgattcag gatggtcgga gagcagctcc accagcggat cgctgaagct ttgccatagg
    27541 ctgctctgga caaaagtgcc gccaccctgg cgacgaagca acaagccctt cgcttcgaga
    27601 cgttgaatcg cctcacgcaa ggagggacgg gagacgtcga actgttttgc cagttcgcgt
    27661 tccggtggga gtttttcgcc cgggcggaga gtgccttcga ggatcaaaaa ctccagttgc
    27721 tgctcaatca catcggagag ttttggttgg cggattttgc tgtaggccat gagttcctgt
    27781 cttaagccac ttgccgaagt caattggtct taccaatttc atgtctgtga cgctaaagta
    27841 acaaagtatt caccttatgt ccatacaggt tttgattgaa atcatgaaac tgtgcacatt
    27901 ttaacaactt gacatatata acgtttcaaa gttgtaacta tgcacaaatg tagactttac
    27961 gtaggaaagg gattttttaa cgattattaa actataaaaa tccgaattga accgattcac
    28021 ttaccaattt tttgattttt aagtcaataa aaacgaattt aaattcactc tgcatattga
    28081 gaggggttga ggctgagctt tacaaacggt ttctttttaa gcaactcatc ttcaaccatg
    28141 cataaagcgg gtgcattcgc tgccgcatac cattattctt gatctgacgg aagtcttttt
    28201 gtaacaattc aaacctcctt gatgtaaaca aatcaataca acaaacggaa ttgcaaactt
    28261 acacacgcat cactgcgtag atcaaaaaaa caatcaccgc acgaggtttc atgatggaag
    28321 gtcaacagca cggcgagcag ctaaagcgcg gccttaaaaa ccgccatatt cagcttatcg
    28381 cgctgggtgg cgcgataggg acagggctat tcctaggtag cgcctccgta atacagtccg
    28441 cagggccagg gattatcctg ggttacgcca ttgctggttt tatcgccttt ctgatcatgc
    28501 gtcagctggg tgaaatggtg gtcgaagaac ctgtcgcagg ctcctttagc cactttgctt
    28561 ataaatactg gggcagtttt gccggtttcg cctctggctg gaactactgg gtactgtacg
    28621 ttttagttgc catggcagag ctgactgccg tgggtaaata cattcagttc tggtatccgg
    28681 aaatcccaac ctgggtttct gccgccgtat tctttgtggt gattaacgcc atcaacctga
    28741 ccaacgttaa agtgtttggc gagatggagt tctggtttgc cattatcaaa gttattgcgg
    28801 tagtagcgat gatcatcttc ggcggctggc tgctgttcag tggcaacggc ggcccgcagg
    28861 cgagcgttag caacctgtgg gatcaaggtg gtttcctgcc gcacggcttc accggactgg
    28921 tgatgatgat ggcgattatc atgttctcgt tcggtggtct ggaactggtg gggatcaccg
    28981 cagcagaagc tgataacccg gagcaaagta tcccgaaagc aactaaccag gttatctacc
    29041 gcatcctgat tttctatatt ggttcgttag ccgttctgct ctcactgatg ccgtggaccc
    29101 gcgttaccgc cgataccagt ccgtttgtgc tgatcttcca cgagttaggc gatacctttg
    29161 tggcgaatgc gctgaacatc gtggtactga ctgcggcgct ctccgtgtac aacagctgcg
    29221 tatattgcaa cagccgtatg ctgtttggtc tggcacaaca ggggaacgcg ccaaaagcgc
    29281 tggcgtctgt cgataaacgc ggcgtaccgg ttaacaccat tctggtgtct gcgctggtta
    29341 cagcattgtg cgtattgatt aactatcttg ctccggaatc cgcatttggc ctgttaatgg
    29401 cactggtggt atccgcactg gtgatcaact gggcgatgat cagtctggcg catatgaagt
    29461 tccgtcgcgc caagcaggaa caaggcgtgg taactcgctt ccctgctctg ctttatccgc
    29521 tgggtaactg gatctgcctg ctgtttatgg cggcggtact ggtgattatg ctgatgaccc
    29581 caggaatggc gatttcggta tacctgatcc cggtatggct gatcgtgtta ggtatcggct
    29641 atctgtttaa agagaaaacc gccaaagccg taaaagcgca ttaatctctc tacgccctca
    29701 cccgtacagg gtgagggcaa taatctctac accaacgccc cgtaaatcgt cagtagcgca
    29761 atcaccacca cgaccacgaa cgaggttttc ttcgccattg aaaccgctgc cttcggcgtc
    29821 tccaccttat cgacatgcgg ttcacgcgcc agagagaact gcgccagacg cgttaatacc
    29881 tgatattgcg aagtatggaa atcacccagc gaagcaaacc aggccggtaa cgctttctca
    29941 ccatgaccga tcaaggcata taccacaccc gcaagacgaa ccggcaccca atccagtaca
    30001 tgaagcactg catcaatgcc ggactgtaaa cgatgatgcg gcgtctggta tcgcgccagc
    30061 cagtattgcc atgcacgcaa gaacgcatac cccatcagcg taacgggtcc ccaggttccc
    30121 cccacaatca gccagaacag cggtgcaaga taaaaacgaa agttaatcca cagcaatgca
    30181 ttttgcagct cacgcaaata ctcacgttcg tcgcagcctg ccgggacgcc gtgaatcatg
    30241 gtgagttcgc cagccatcgt ggcacgggca tggctatcat tacgtgaagc agctgtcaga
    30301 taagcatgat aatgaagacg aactttacct gcgccaatac acagcaaacc aatcagcagc
    30361 cacaccagca gcgtgggaac gttgaacaat actccctgca atgcgcgtaa cagtaaaaaa
    30421 gtcacaccca tcgcaataat ggtcatgcct aacgtgcgcc cgagagaaaa atgtttcacc
    30481 cgccgaaaga acgcttcaag acgatgatca agctgccagt gctcgcccaa cttaaacagg
    30541 cgctcgaaaa ttaacaccag taaggttgta aatagcgtca tgttgtctcc ttgctgacca
    30601 gcgcacgaaa ccgtgcccaa tcaaatgcag gaccgggatc ggttttccga tccggcgcaa
    30661 tatcacaatg gcccgtcatg ttattagcga tatcgggata gcgatctatc agtgcccgcg
    30721 taaccgccgc aagctgttga tactgcgcat cggtatacgc cagagtatcg gtgccttcaa
    30781 gctcaatccc aatagaaaaa tcattgcagc gttcacgccc ctgatactga gagactcccg
    30841 catgccaggc acgtttatcg aaaggaacat actggactat ttcaccatca cggcgaatca
    30901 aacagtgagc ggagacgcgc aaatgggcga tctcagcaaa gaaaggatgt gcctgcggat
    30961 caatagttcc agtgaataat gcgtcgatcc acggaccgcc aaactcgcct ggcggcaggc
    31021 taatattgtg caccaccagc agggtgggtg tttcgtcatc caggcggcaa tcgtaatgtg
    31081 gtgagggaac gcggcgcgcg ccaaccagcc acccctgttc taacaacatg caggatctcc
    31141 ttatatgtgg tgctaatacc cggttcagag tagcatgttt ctaccttatg attcgttagc
    31201 tatctggagt tttaacatgc cgcctctccg ctacaaccct gacacccgac gtgacgagct
    31261 gctggaacgc attaatctcg atatccccgg cgcggtggcc caggcgctgc gggaagattt
    31321 aggcggaaca gtcgatgcca acaatgacat tacggcaaaa cttttaccgg aaaattctcg
    31381 ctctcatgcc acggtgatca cccgcgagaa tggcgtattt tgtggcaaac gctgggttga
    31441 agaggtgttt attcaactgg caggcgacga tgtcaccata atctggcatg tggatgacgg
    31501 cgatgtcatc aacgccaatc aacccttgtt cgaacttgaa ggcccatccc gcgtgctgtt
    31561 aacgggcgaa cgcactgcgc ttaattttgt gcaaaccctt tcaggagttg ccagtaaggt
    31621 acgccactat gtcgaattac tggaaggcac caacacgcag ttgttggata cgcgcaaaac
    31681 cttacctggc ctgcgttcag ctctgaaata cgcggtactt tgcggcggcg gagcgaatca
    31741 ccgtctgggg ctttctgatg ccttcctgat caaagaaaac catattattg tctccggctc
    31801 agtgcgccag gcggtcgaaa aagcgtcctg gctgcatccg gatgcgccag tagaagtcga
    31861 agtagagaat ctggaagaac ttgatgaggc cctgaaagcc ggagccgata tcatcatgct
    31921 ggataacttc gaaacagaac agatgcgcga agccgtcaaa cgcaccaacg gcaaggcgct
    31981 actggaagtg tctggcaacg tcactgacaa aacactgcgc gaatttgccg aaacgggcgt
    32041 ggactttatc tccgtcggtg cgctaactaa acacgtacaa gcactcgacc tttcaatgcg
    32101 ttttcgctaa ttgctgacag atttatccgc tccatcgcgg gcggataatc tccccttccc
    32161 caactttctt cgtaacgcct cgcaaatttc ttgtctttcg ctgcaacgaa ataaaaaacc
    32221 tcggaatctg ctttcccgtt tcgttttttg cccctcgcct gtgctgatgg cgtttgccaa
    32281 agtagcacca accaaatcaa ggagcgaaac agatggacaa gcaacgcggt tttacactta
    32341 tcgaactgat ggtggttatt ggcatcattg ccattttaag ctccattggt attcccgctt
    32401 atcaaaacta cctgcgcaaa gccgcactca ccgacatgct acaaaccttt gtgccttacc
    32461 gtaccgccgt agagttgtgc gcgctggaac atggtggatt agatacctgc gacggtggca
    32521 gcaatggcat tccctcgcct accaccaccc gctatgtttc agccatgagt gtggcaaagg
    32581 gcgtggtgtc gctgaccggg caagaaagtc tcaatgggct aagcgtcgtc atgacgccag
    32641 gttgggataa cgcaaacggc gtcaccggct gggcgcgcaa ctgcaatatt caaagtgaca
    32701 gcgcattgca gcaagcctgc gaagatgtct tccgctttga tgacgccaac taaggagcgg
    32761 caatgaatat tccacagctc acggccctgt gcctgcgtta tcagggagtc ttgctggatg
    32821 ccagcgaaga agtggttcat gttgcggtgg tcgatgcccc ctcacatgag ttgctggacg
    32881 cattgcattt cgctaccacc aaacgtattg agatcacctg ctggacgcgc caacaaatgg
    32941 aaggtcacgc cagtcgcaca caacagacat tacccgtagc tgttcaggag aagcatcagc
    33001 ccaaagcaga gttgctgact cgaacgttac aatctgcgct ggaacaacgc gcgtctgata
    33061 ttcatatcga accagcggac aatgcctacc gcatccgctt gcgtatcgac ggcgtattgc
    33121 atcctttacc ggatgtttca ccggatgccg gagtcgcatt aacagccaga ttaaaagtgc
    33181 tgggaaacct ggatattgcg gaacatcgcc tgccgcagga cgggcaattc actgtcgaac
    33241 tggcaggaaa caccgtctca tttcgtattg cgaccttacc atgtcggggt ggtgaaaagg
    33301 tggtattaag gttgttacag caggtgagcc aggcactgga tgttaacacg ctgggaatgc
    33361 agccgttaca actggcggac tttgctcatg ccttgcaaca accacaggga ctggtgctgg
    33421 taactggccc tacaggcagt ggcaaaacgg tcacctggtg ttgtccacgc tacacaccaa
    33481 ttccaccact gaaacgctgg tacgtttaca gcaaatgggg gtcgcccgct ggatgctatc
    33541 atcggcgctt acgctggtaa tagcccagcg tctggtacgt aaactttgcc cacattgtcg
    33601 ccgacagcaa ggggagccca tccatattcc agacactgta tggccgtcgc cgctgcccca
    33661 ctggcaggca cccggttgtg tacattgcta ccacggtttt tatggtcgta ccgccttgtt
    33721 tgaagtcctg cccattacac cagccatacg ccagcttatt tccgctaata ctgaagttga
    33781 atcgctggaa acgcacgccc gacaggcggg tatgcgtacg ctttttgaaa acggctgcct
    33841 ggccgtggag caaggcttaa ccacctttga agagttaatc cgcgtactgg ggatgccgca
    33901 tggcaagtaa gcaactctgg cgctggcatg gcataaccgg cgacggcaat gcgcaagatg
    33961 ggatgctatg ggcagagagc cgtactttac tgcttatggc actacagcaa cagatggtta
    34021 ccccactaag cctgaagcga atcgccatca attctgcgca gtggcgagga gataaaagcg
    34081 cggaagtcat tcatcaactg gcgacgctac ttaaagccgg gttaacgctt tctgaagggc
    34141 tggctctgct ggcggaacag catcccagta agcaatggca agcgttgctg caatcgctgg
    34201 cgcacgatct cgaacagggc attgcttttt ccaatgcctt attaccctgg tcagaggcat
    34261 ttccgccgct ctatcaggcg atgatccgca cgggtgaact gaccggtaag ctggatgaat
    34321 gctgctttga actggcgcgt cagcaaaaat cccagcgtca gttgaccgac aaagtgaaat
    34381 cagcgttgcg ttatcccatc atcattttag cgatggcaat catggtggtt gtggcaatgc
    34441 tgcattttgt tctgccggag tttgccgcta tctataagac cttcaacacc ccactaccgg
    34501 cactaacgca ggggatcatg acgctggcag actttagtgg cgaatggggc tggctgctgg
    34561 tgttgttcgg ctttctgctg gcgatagcca ataagttgct gatgcgccgc ccgacctggc
    34621 ttatcgcgcg gcagaaattg ctgttacgca tcccgattat gggttcactg atgaggggac
    34681 aaaaactcac gcagatcttt acgattctgg cgctgacaca aagtgcaggc attacttttt
    34741 tacagggcgt agagagcgtc agagaaacaa tgcgctgccc gtactgggtg caacttctga
    34801 cacaaatcca gcacgatatc agtaacggtc atcccatctg gctggcgcta aaaaatgccg
    34861 gtgagtttag cccgctctgt ttgcaattag tgagaacagg agaggcatcc ggctcgctgg
    34921 acctcatgtt agacaacctc gcccatcatc atcgggataa cacaatggcg ctggcggata
    34981 acctcgcagc cttactggaa ccggcgttgc tgatcataac gggaggaatt atcggtacgc
    35041 tggtggtggc aatgtatctg ccaattttcc atttaggcga tgcgatgagt gggatgggat
    35101 aacgctggcg tgttgctcca cgccagcgtt gggagattac aggttgttga agatgcggtt
    35161 ttcttgttcc agcacacgaa taaacgtggt gcgcttggtc agctctttca ggcgtgaagc
    35221 cccaacgtat gtacaagctg aacgcaggcc gcccaaaata tcacgcgcgg tattttcaac
    35281 ctggcctcgc agcggcagct taacggtttt accttctgct gcgcggtatt ccgcaacgcc
    35341 gccaacgtga cgtttcatcg cagactcgga gctcatgccg tagaacagca taaatttctc
    35401 gccgttctcc tcaacgatgc gaccgccgct ctcttcgtgg cccgccagca tgccgccaag
    35461 catgacgaaa tcggcaccgc cgccaaaggc tttcgccaca tcgcccggcg tggtacagcc
    35521 accgtcgctg acgatcattc cgcccagacc gtgcgccgca tcggcacatt cgattaccgc
    35581 agaaagctgc ggataaccga cgcctgtttt gacgcgagtt gtacaaacag aacctgggcc
    35641 aatgccaact ttaacgatat cagcaccgga gaggataagc tcctcacaca tttcaccagt
    35701 cactacgttg ccagcacaaa tggttttggt cggccacgct tcacgcgctt tcgcaacgaa
    35761 ctgcacgaag tgttcggaat aaccattcgc cacatcaata caaacgaagt ttaatgccgg
    35821 gttcaggtcg agaatctgtt tagttttttc gaaatccgca tcagacgtgc cggtagaaac
    35881 catcacatgt ttcagcacat cagcggaaga attgttgata aacgcttgcc actcttcgac
    35941 agaaaagtgt ttatgcacag cagtcagaat atcaaaagaa gccagcgcag aggccataga
    36001 aaatgtgcct acggtgtcca tatttgcggc gataatcggc acgccggacc agctctgacc
    36061 tgaatgtttg aaggtgaatt gacgttccag ttcaacatcg gaacggcttt taagagtgga
    36121 gcgtttaggg cggatgagaa cgtctttaaa acctaacttc agatcttctt caatacgcat
    36181 gtgcggattc ctggggttaa tggctataag ggtaagagcg caactccagt gacgctatca
    36241 tacgcactaa tcaatgcggc gcaagactgc gaaattgtct ttttttacgc tacaatccca
    36301 taaatttacc tggtaaatag tgagcgaacg tttcatcaat ttcactttcg cgcgctcatt
    36361 tttaacgttt tgataatcat gatgaattcc cgggaatata aattatgggg tatatagttg
    36421 ccttaacggg aggcattggc agtggtaaga gtaccgttgc caatgcgttt gctgatctcg
    36481 gaattaacgt cattgatgcc gatattattg cgcgtcaggt ggttgaacca ggtgcacctg
    36541 cgctacatgc cattgctgat cactttggcg aaaacatgat tgctgctgat ggaacattgc
    36601 agcgccgggc cttgcgcgag cggatcttcg ccaacccgga agagaaaaac tggcttaacg
    36661 acctgctgca tccgctgatt caacaagaga cgcaacacca gatccagcaa gcaacttccc
    36721 cctatgtact gtgggttgtg ccattgctgg tagaaaactc actgtataaa aaagcgaatc
    36781 gagtgctggt ggtggatgtc agcccagaaa cgcaacttaa gcgcaccatg cagcgcgatg
    36841 atgtaactcg cgaacatgtc gaacaaatcc ttgctgctca ggcaacgcgc gaagcccgcc
    36901 ttgccgtggc agatgacgtc attgataata acggcgcacc ggatgctatc gcatcggatg
    36961 ttgcccgcct gcacgcacac tatttgcagc ttgcgtcgca gtttgtctca caggaaaaac
    37021 cgtaatgcag acccaggtcc tttttgaaca tccactaaat gaaaaaatgc gtacatggct
    37081 gcgcattgag tttttgattc agcaactcac cgttaattta cccatcgttg accacgctgg
    37141 cgcgctgcat ttcttccgta atgtcagtga attactggat gttttcgagc gcggcgaagt
    37201 ccgcactgag ctgttgaaag aacttaaccg gcagcaacgt aaactccaga cctggattgg
    37261 cgtgcctggc gtggaccaga gccgtattga agcattaatt cagcagttaa aagcggcggt
    37321 gagcgtatta atttccgcgc cgcgtatcgg gcaatttctg cgtgaagatc gtttgattgc
    37381 tctggtgcgt cagcgactga gcatcccagg cggctgttgc agctttgatt tacctacatt
    37441 gcacatttgg ctgcatctac cccaggcgca gcgcgacagc caggtagaaa cctggattgc
    37501 cagcctgaac ccgctcaccc aggcacttac catggtgctg gatttaattc gccagtcggc
    37561 ccccttccgt aaacaaacca gcctgaatgg tttttatcag gataacggtg gcgatgccga
    37621 cttgctgcgc ctgaatctgt cgctcgattc acagctttat ccgcaaattt ccggacataa
    37681 gagccgtttt gccattcgtt ttatgccgct ggacactgaa aacggacagg taccggaacg
    37741 tctggatttc gaactggcct gttgctaagg agtaaaaatg tcagaaacta ttacggtgaa
    37801 ttgcccaacc tgcgggaaaa cggtggtgtg gggtgaaatc agcccgtttc ggccattttg
    37861 ctccaaacgt tgtcagctga tcgacctcgg agaatgggct gctgaagaaa aacgaatccc
    37921 cagcagtggc gatctttccg aaagcgatga ctggagcgaa gagccaaagc agtgacattt
    37981 gtctgatgcc tcacgtaggc ctgataagac gcggacagcg tcgcatcagg catcttgtgc
    38041 aaatgtcgga tgcggcgtga acgccttatc cgacctacag acgcttaagc ttcgcaatta
    38101 ccggttcatt ggctggcgga aaatcatcgg cattaagacc gaccagcgac atccactcac
    38161 cgggttgccc ttctttaccc cacggccccc cttcccaact ttcgaccagc cagaaccaca
    38221 gagttatatg cctgtccggg aattcatatt ccagtttttc aaatagcgaa aaatgttgtg
    38281 gggtaatccc gacctcttcc tgaagttcac gcaccaccgc ctgttccggc gtttcgccca
    38341 tttcaatttt accgccggga aactccagtt tattcgccat gtgcgcatct gctgcgcgac
    38401 gtgttataaa gatttcattg ttctcgttgc gaataatacc taccgcaatt tgcagctttt
    38461 tcattgtctt aaacctataa aaaaggcgca gaatcctgcg ccttttactt caactgttag
    38521 cttttattgc aggcgaccat ggcactgctt gtatttttta ccagaaccgc acgggcaagg
    38581 atcgttacgt cctactttgc gttcaccggt ttgcgccgcc agtgcagctg cggctgcaga
    38641 gtcgtcatcc tgatggctaa gctgctgcat ttgtgctaaa cgctcggctt ccatacgacg
    38701 ctgttgttcc agctcctcaa cctcttcagg catacgtacc tgaactttgc tcagcgtact
    38761 gataacttca tatttcaacg actccagcat cgctgcaaac atggagaacg attcacgttt
    38821 gtattcctgc ttcggatctt tctgtgcata gccacgcagg tggataccct gacgcagata
    38881 gtccatcgct gccaggtgct ctttccacag ggagtcaagc gtttgcagca tgacgccttt
    38941 ctcgaagtga cgcatcatct cagcaccaac cacttcttct ttacgctgat acacttcgat
    39001 ggactgcgcc agaatgcgct cacgcagcgt ctcttcatgc agttctggtt ctttatccag
    39061 ccactcggta attggtaaat cgaggtcgaa atcgttcttc agacgttcct gcagccccgg
    39121 aatatcccac atttcttcca gcgactgtgg cggaatgtag gcatcaatgg tggctttgaa
    39181 cacatcttca cgaatgctgt tgatggtttc gctcacatcg ctgacatcca gcagttcgtt
    39241 acgctgggag taaatggcgc gacgctgatc gttagccacg tcatcatatt ccagcagttg
    39301 cttacgaatg tcgaagttac gactttcaac tttacgctgg gcgttggcaa tcgctttagt
    39361 cacccacggg tgttcaatgg cttcgcctgg cttcataccc agtttacgca tcatgccgga
    39421 tactcggtcg gaagcaaaaa tacgcatcag cgcatcttcc atcgacaggt agaaacggga
    39481 agaaccagca tccccctgac gaccagaacg accgcgcaac tggttatcga tacgacggga
    39541 ttcgtgacgc tcggtaccga tgatatgcag gccacctgct tccagtaccg catcgtgacg
    39601 tacctgccag tcggctttaa ttttttcaat ttgctctgcg gtcggatttt ccagcgcggc
    39661 aacttctgcc tgccagctac ctccgagcac aatatctgta ccacgacccg ccatgttggt
    39721 cgcgatagtc accgcagccg gataacctgc ctgagcaaca atcgccgctt cgttggcgtg
    39781 gaatttggcg ttcaggacat tgtgcttaat accggctttg gtcagttcgt ttgacaccag
    39841 ctccgatttt tcgatggaga tagtacccac cagcaccggc tggcctttcg cagtacgttc
    39901 tttgatatct tcaatgatcg cctgaatttt ttccgcttca gtcatgtaga ccaggtccgg
    39961 cagatcttta cgaatcattg gacggttggt cggaacaacg acggtatcca gtttgtagat
    40021 ggagctaaat tcgaaagctt cggtatcagc agtaccggtc atccccgcca gtttttcata
    40081 cagacggaag tagttctgga aggtgatcga agccagcgtt tggttttcgt tctggatctg
    40141 cacaccttct ttcgcttcca cagcctggtg cagaccatcg gaccagcgtc ggccctgcat
    40201 ggtacgaccg gtgtgttcgt caacgatgat aacttcacca tctttaacga tgtagtcgac
    40261 gtcacgggta aacagcgcat gagcgcgcag cgccgccgtt acgtggtgca tcagcatgat
    40321 gttggccgga gagtacagag actccccttc atccatgatg ccctctttca ccagcagttc
    40381 ttcaatcagc accagaccac gttcggtcag gttcacctgg cgagattttt cgtccaccga
    40441 gaagtggcct tcgccctgga aggtttcgga gtcttctttt tcctgacgga tcaggtgcgg
    40501 aataatttta ttcacgcgtt tatacatttc cgagctgtct tctgccgggc cggaaatgat
    40561 cagcggtgta cgcgcttcat cgatcaggat ggagtccact tcgtccacca gcgcatagtg
    40621 cagtttacgc tgtacacgtt cttcagggct gaacgccatg ttgtcgcgca ggtagtcaaa
    40681 gccgtattcg ttgttcgtac cgtaagtgat gtcagctgcg taagcttcgc gctttgccgg
    40741 tgctggcatg cccggcaggt tgataccaac agtcaggcca aggaattcaa acagcggacg
    40801 gttgttttcg gcgtcacgtt gcgccaggta gtcgttgacg gtaactacgt gcacgccttt
    40861 accggttagt gcgttcaggt aagcaggcag cgttgcggtc agggttttac cttcaccggt
    40921 acgcatttcg gcgatgcagc gttcgttaag aaccataccg ccgagtaact gaacgtcgaa
    40981 gtgacgcata ccaaagacgc gcttacttgc ctcacgcacc acggcgaaag cttccgggat
    41041 cagattttcc agcacttcgc ctttttccag acgcgcacga aactctgcgg ttttcccttt
    41101 cagttcttca tcggagagtt tttccatctc cggttccatg gcattgatga tgttgaccac
    41161 tttgcgcatc cggcgcaggg tgcgatcgtt acgactaccg aaaactttag ttaacaattt
    41221 gattagcata ataaaatctc aaacgccccg cgttacggag ttaataaaat gaagtaaagg
    41281 tttattgttg ttaggtgagg cgttgagggc cagcacggat gccttgcgcc tggcttatcc
    41341 agacgggcgt gctgaatttt gcttgtgggg taaaatgcgc ataatcaatg cgataaccct
    41401 tttcagacgg cgtgccttcc tgggtcagca gcgcgctgag cgtatccagt aatgcaagat
    41461 gttgcgcctg aagaggcaaa gattcttcag caacgggcag tgtttgcggt gccattgcga
    41521 aagaaagatg acggattacc gtacgaatag catgttgatg ccagtaatca acggaatagt
    41581 tcgaattcgg gcggcgcgtg ttcgcttcca gcaaggccaa ttgaccaaag ttaactttgg
    41641 ctgaaggctc gtggttgcgg gttgtcgctt ttgcgggcgc gtttggttcg gcggcgttgc
    41701 tgagcgcagg caaacctaaa ctcgccgcga ccatccctaa taagagatgc ggccagaagt
    41761 agcgtttacc aaactgtcgc cagcgcgtca gtattccact cacgttattg ccatcccgat
    41821 cgaattcaaa cgtatttagc gcacaaatct tatcattaaa gcctgaagtc cacagcacga
    41881 atgaagataa gttgtgcgta aaagtgctta agaaaacagc gttcgcaccg ttttttatag
    41941 ctcttataaa attcaaaaaa aaacgactgg ttcacctggc cggagagagt gccagatttg
    42001 ccagtcgaat tttatacgac agtataaatg tcgttatgcc agtacagctg aaggcgcttt
    42061 gaaggccaac ggcagttctg cgtcgtcctg gaaggtcaca tattcccagg cttcctgttt
    42121 cgccaggaca gcctgcagca gtttgttatt cagtgcatga ccggatttat aagcggtaaa
    42181 tgcaccaata atattgtgac cacacatgaa caagtcaccg atcgcatcga gcattttgtg
    42241 acgcacaaat tcgtcttcaa aacgcaggcc gtcttcgttc agtacgcgat aatcgtcaac
    42301 aacgatggca caatcgaagc tgccgcccag gcacaaacca cgggactgca gatattcgat
    42361 atcacgcatg aaaccgaacg tacgcgcacg gctgatctgg cgcataaacg catcagcgga
    42421 gaagttcatc gcatagcgct ggttgctgga atcaatagcc ggatggttaa aatcgatggt
    42481 gaaatccagc gaaaaaccat tgtacggctt aaattcagcc cacttatcgc catcttcgac
    42541 acgaacagtc tctttgatgc gaacaaattt tttggcgcag ttcaactcgt cgataccggc
    42601 gtcaagcagc aggtatacaa acggagcggc gctgccgtcc atgatcggga tttccggcgc
    42661 gttaacttcg ataacaatgt tatcgatgcc caggcccgcg agagcagcat tgaggtgctc
    42721 tacggttgaa atccgtacat catgctcgtt gaccagacac gtacagagca tggtatcacg
    42781 cacagatttg gcatcggccg ggaaatctac cggtggattc aagtcggtgc gacgatagat
    42841 gaccccggtg ttggccggcg cagggcgtaa cgtcagggtg actttcttgc cggtatgtaa
    42901 accgacaccc gtcgcctgaa cgatacgttt aagtgtcctt tgtttgatca tcgtattatc
    42961 tcgccaaatt acctatccaa ccgaagtgta ctatacattc ggcgggccag tttagcacaa
    43021 agagcctcga aacccaaatt ccagtcaatt cttaatcagc ttgcttacgt aggaatgctg
    43081 ggatatccag ataatccggc tctttcgcag tttgcggcgc attgtcattc actactttag
    43141 caaccggctt ctgctcctgg gtcagcggag ccatcccatg ctgctggtag cgatccatca
    43201 ctggctgctg aacctgctta ttggtcacca gagtgatttc aggacgtttg tccatgccga
    43261 tacctgtcgc aacaacggtt acgcgcagct cgtcattcat atccgggtca agagaagtac
    43321 cgataaccac agtcgcgttg tcggaagcaa atgcacggat ggtgttacct accgtttcga
    43381 actcatccag acgcaggtcg aagcccgccg tgatgttaac cagcacgccg cgcgcgccag
    43441 acaggtcgat atcttccagc agcggagaag agatagccat ttcagcagct tcttccgcac
    43501 ggtcttcacc gctcgccacg ccagaaccca tcattgcgta gcccatttca gacattacgg
    43561 tgcgtacgtc tgcaaagtcc acgttcatca aacccggacg agtaatcagt tcggcgatac
    43621 cttgcacagc gcctttcagt acatcgttcg ctgcgccaaa cgcatccagc agggagatac
    43681 cgcggcccag aactttcagc agtttgtcgt tcgggatagt gatcagagag tccacatgct
    43741 tggacagttc agtgatcccc tgctcggcga atgccatacg cttcttgcct tcaaagttga
    43801 aaggcttagt gacgacagca acggtcagga tacccaaatc ttttgccact tcagcgacga
    43861 ctggtgctgc acctgtaccg gtaccaccac ccatacccgc agcaataaag accatgtctg
    43921 caccttccag cgccgcacgc aatgcatcgc gatcctcatc agccgcattg cggccaactt
    43981 ctggattagc gccagcgccc agtcctttgg tgataccgct accgatttga atcgtctgtc
    44041 caaccgctgt tttacgcagc gcttgtgcat cggtatttac cgcgaagaat tcaacacctt
    44101 caatacgctc gcgcaccatg tgttcaacag cattaccgcc gccgccgccg acgccgatga
    44161 ctttaatcac cgcgtcattg gtaagttcca ttggttcaaa catagtttct ctccgatttg
    44221 tgcctgtcgc ctgagtccgt aatcatcgtc ggcctcataa aaattaaaac tcttttcgca
    44281 gccaactatt gagtcgcttg atccacgagc caactgatgc tgtaacacgt ttttctactt
    44341 cagcttcacc gttaagatgt gactctttcc catagtgaag caatcccacc gccgtcgaat
    44401 aatacggctc ctgagcataa tccgttaaac cggtaatgtt cagcggcgcg ccgatacgca
    44461 cttgcgtatg aaacacgcgc tgagcacagg ctgcaagacc ttcgatctgc gctgcgccac
    44521 cggttaatac aatgcctgcc gccaggtgat gtttaacccc ttgttggcga agcttttcct
    44581 gcaactgcaa tatctcttcg ttgaccaggt tgagcaactc ggtatagcgc ggctcgatca
    44641 cctctgccag ggtctgacgt tgcagactcc gcggcggacg accgcctacg ctcggcactt
    44701 ccacgctctc atcttttcca acgatggaac ccagcgcaca accgtggcga actttaatcg
    44761 cttcggcgtc gcttggcggc gtgccaaagg cgtaagcgat atcactggtc acgacattgc
    44821 cagcataagg aattaccttt gtgtggcgca atgccccacc ggtataaacg gcgatatcca
    44881 ttgtaccacc accgatatcg acgacgcaga cacccagttc acgttcatct tccgtcaata
    44941 ccgaataact tgatgccagt ccggcaaata tcagttggtc aactttcagc ccacaacgtt
    45001 caaccgcttt gacgatgttt ttcgccatat cgttgtgaca tgtgatcagg tgcacttttg
    45061 cctgcatccg cacacccgaa agtcctaccg gattcttgat cccttcctga tagtcaatcg
    45121 catactcttg cgggatcaca tgcagcacac gatgctcatc gcgcacacgc accgatttcg
    45181 cggtatggac gacgttttcc acatcttctt gcgtcacttc ttcttcagaa ataggcacca
    45241 taccaatttc attctggcag ctgatgtgct taccagaaag cgccagatat accgaagaga
    45301 tctgacaatc tgccatcaat tctgcctggt caatggcgcg ttgtacgcac ttgaccacgg
    45361 attcgaggtc gttcaccccg cctttatcca taccacgtga cgggcagctg cccacgccaa
    45421 tgatattgac cataccgtcg ggcagaactt cccctactaa agcggcaacc ttcgcggtac
    45481 caatttccag tcctactacc agttttctgt ccgtcgcctt gatcattgtt gttctgcctg
    45541 tgcctgattt tgttgctgag tagattcctc tggcggcaag ggcgcccagc ctactgccgc
    45601 tccagagtca taacgcaaat caacgtagct aatccgtttg ccatcggttt gcgcctgctg
    45661 ctgtaaaacc ggataaagtt ctacaaagcg aaccaaacgt ttcatcgtat cgccccggcc
    45721 aagattgagc ttaatatcgt tattcagcgt caactgccag gaacgccgcg cggtcatcgc
    45781 cgcttccttc agagtaaatc tgtcctttgc cagcatctgc cccatttcgc gatagccctg
    45841 caacacttca ttagcgctgc cttccgggcc atacagcatt ggaagcacct gcttgctggt
    45901 gcgatctggc ggcacgctga aggtgtttcc ttccgcgtct accatgtgtt gatcattcca
    45961 ccgcgcaatc ggcacatatt caaccagatg aatcttcaat tcatcaggcc actgctttct
    46021 gacgctcacc tgcttaatcc acggcaggcg ttgttctatt tgcgtctgga tgatgttgac
    46081 atcctgggtc ataaaggtac ccggctcacc caatgccagg atcgactgcc ggatatcgtc
    46141 attacgcgtg taatggcgtt caccggtcaa caccagcttt gagagcggca ggcgttgcgc
    46201 atcttccatc cagctcaaca cgacccagcc gctcaccaac actgtcgtta aaacggtcag
    46261 caggaaaagg atccccgcca gacgcgttcc attattgcgg cgagaagaaa cctcttcttc
    46321 gctgtttcgc gtgttcagag cagcctgcga catattagtc cgccagttcc agaattcgta
    46381 ctaccaactg cgagaagctc atacctgcct gacgtgccgc catcggcacc aggctgtggc
    46441 tggtcatacc cggtgaggta ttggcttcca gcagataaaa ctgtccatca ctgtccagca
    46501 taacgtcaat acgtccccat cctttgcaac ctaacgtcgt ccatgctttc agcactaatg
    46561 cctgcaaatt ggcctcttgt gacgcttcca gacctgcggg gcagaaatac tgtgtctcat
    46621 cagagagata cttcgcctca taatcataga aggttccgga cggttgaata cgtattgacg
    46681 gtaaaatttc ttcaccgagt atcgcaaccg tgaactccgg cccacttagc catttttcaa
    46741 tcaatacttc ttcatcgtgc tgaaatgcca atcttaatgc atcttgtaga gcattttctg
    46801 ctactacttt tgacattccc acactggaac cttcgcggct cggcttaacg ataaccggca
    46861 aacccagagc agaaatttct gctaactgct tatcgctcag gcctttttca aactctgcgc
    46921 gggttaacgc tacccacggc gcgaccggta aaccggcacc ttgccacaga agtttgctgc
    46981 gtagtttatc tattgaaagc gcagatgcca tcactccgct gccggtataa ggcaagccca
    47041 tcagctcgag catgccctgc agcgtaccat cttcaccgcc gcgaccgtgt agcgcgataa
    47101 acactttctg aaaacccatc gacttcagtt gcgtcacgtc gacttctttc gggtcgacag
    47161 gatacgcatc aataccgcct tcacgcagcc cggctaacac cgctgcgcca gaattcagag
    47221 aaacttcccg ctcagcggaa gtcccgccca acaggaccgc gattttatca gtcatgttgt
    47281 tcttcctccg gagtttgcgg cttcagtttg atttcagcta aagaacgggc aatttttccg
    47341 atattaccag ccccctgaac gagaatcagg tcgttaccgg ttaataccgg tgccagcatc
    47401 tcggctacct gcgcagggtc cggcaccaga atgggatcaa ttttcccacg tccacgaatt
    47461 gtgcgacaca gcgaacggct gtccgctccc ggaattggcg cttcgccagc cggatacact
    47521 tccagcatca acagggtatc aacctgcgtc agcacattgg cgaaatcatc atacaggtcg
    47581 cgcgtacggg taaaacggtg cggctgaaac agcattacca ggtttttatc cggccagcct
    47641 gcgcgcgccg ctttaatggt ggcgtcaact tccgtcgggt ggtggccgta gtcatcgacc
    47701 agcattgccg taccgctttt accattcact ggctccagcg ggaattcacc gaggaaatca
    47761 aaacggcgac cagtcccctg gaagctctcc agcgcacgca aaatagcctc gtcgtcaatt
    47821 ccctcttccg tagcaaccgc aaccgcagct gcggcgttca gcgcgttatg acggcctggc
    47881 gcattcaggg tgacgcacat cggctctttg tcctgacgca gcagcgtaaa gtgcccctgc
    47941 gggccaatct gctgataatc ttctacacgc acgtcggcat cttcgctgaa gccgtaagtt
    48001 gtggtctgac gtcccacacg cggtaacaat tcgcggatca ccggatcatc aacacacatc
    48061 accgcacgac cgtaaaaagg caggttgtgc agaaaattaa taaaggtctg ttttaaattc
    48121 tcaaagtcgc cctggtaggt atccatgtgg tcggcttcga tattggtgac aatcgccacc
    48181 atcggttgca gatgcaggaa cgatgcatca ctctcatctg cttcggcaat caggtaccga
    48241 ccatgcccca aacgcgcatg aacccccgcc gcttttacca gcccgccgtt aacgaaggtt
    48301 gggtcgagcc ccgcttctgc gtagatgctg gaaaccatcg cggtagttgt cgttttgccg
    48361 tgcgttcctg caatggcgat gccatgacga aaacgcatta actcagccag catttcggca
    48421 cgacggatca ccggaatacg cgcttcatga gctgcgacaa tttccgggtt gtcggcagaa
    48481 atggcgctgg aaacaacgac cacgctggca tcacgtacgt tttccgggcg atggttgaaa
    48541 taaatcgtcg cacccagatt cagtaactgc tgcgtgaccg gatttggcgc taaatcggaa
    48601 ccactgatct gataaccttc attggccaga acttcggcaa taccgcccat accggcacca
    48661 ccaatgccga caaaatgtat gtgccgaacg cgacgcattt cgggcacgat ggaacgcagt
    48721 tttgccaatt gttgtgtatt cattctttac gccattaact tcttaaattc atacgatgca
    48781 aaaggcatcg ctacaattac gcccgggcag cccggctcac ttcatttgcc actcgctcgg
    48841 tggcatccgg aatggatgca gcgcgggcgc gttctgccat ggttaataag gtttctcgcg
    48901 accacccggc cagggtgttg gcgacagcat ccacgctaag ctgtggctgc tcgataattt
    48961 tggctgcgcc cgctttttcc agcggtagcg cattccagta ttgctggcgg tctttatgtt
    49021 gaaacggcac aaacaacgcc ggtagtcctg ccgcggcgat ttcactcaca gttaaagcac
    49081 cggagcggca aacgacgaca tccgcccacg catacgccgc cgccatatca tcaataaatt
    49141 cagtcacttt atgctgcggt tgccccgctt cggcatacgc ctgttcaacg gattgttgcg
    49201 aacctttgcc gctctgatgc cagatagtga ctgaatcacc cagtttcgca gcaacctgcg
    49261 gcattgtctg gttaagaatg cgtgcgccct gggaaccacc cactaccaac acacgaaccg
    49321 gaccttcacg tccagccaaa cgttgctgcg gcaacggcag cgccagcaca tcggtacgca
    49381 ccgggttacc caccacttcc gcattaggga aagcgcctgg aaacgcctgc atcactttgg
    49441 tagcaatctt cgccagccat ttattggtta agcccgcaat accgttttgt tcatgaagta
    49501 caaccggaat gcctaacgac cacgcggcca gaccacctgg acctgataca tagccgccca
    49561 taccgagtac cacgtcaggt ttgtacgctt tcataatcgc ccgcgcctga cgccaggcgt
    49621 tgaagatacg cagcggcgca gctatcagtg cttttatacc ttttccacgc aaaccagaga
    49681 tacgaatgaa atcaatttcg atgccatgtt ttggcactaa gtccgcttcc atacggtcgg
    49741 cagtccccag ccagcgaact tgccaaccct gagccattag atagtgcgca accgccagtc
    49801 ccgggaatac atgtccaccg gttccgcctg ccatcaccat taatcgcttt ccttgaccac
    49861 tcatcgtgaa cctcgtacaa acgcctgcgc tttctccaga cgcgtttcat aatcaatacg
    49921 caacagcatc atgatggctg tcgacataat cagtaagctc gaaccaccgt aactgattag
    49981 cggcaatgtc agacctttgg tcggtaacat ccccgccgcc gcgcctacgt taaccagcgc
    50041 ctggaagcta aaccagatgc caatagaaca ggcgagaaaa ccggaaaaac ggtggtcaat
    50101 ttctaatgct ttgcggccaa tcgacatcgc acgaaaagcg acgaagaata ccattaaaag
    50161 tgctagtacc acaccgacat aacctaattc ttcgccgata atggcgaaaa taaagtcagt
    50221 gtgcgcttcc ggcagatact ccagtttttg taccgaatta cctaaacctt gcccccaaag
    50281 ttcgccgcga ccaaacgcca tcagcgattg cgttaactga tagccgctgc caaagggatc
    50341 ttcccacggg ttccagaatg cggtaacacg gcggatacgg tacggttcgg cgaggatcag
    50401 caacacaacc gctgaaatgc ccataccgat aatggcaatg aactgccaca atttcgctcc
    50461 cgccaggaac aacatcgcca gcgtagtcac aaacaacacc accaccgtac caaggtctgg
    50521 ctgtgccagc agtaacactg ccaacaccag aatcacgccc atcggtttca ggaagccgcg
    50581 caggttatta cgtacttcgt cgcctttacg caccagatag ttggcgatat agcaaaacag
    50641 cgacagtttt gtcagctccg caggctggat acgcagcaaa ccgagatcga tccaacgcga
    50701 tgccccttta acagcgctac ccactaccag cacgatcatc agcaggatga tagatccgag
    50761 cagcatcgtg gcactgtagc gttgccagaa ctccatcggc agacgcagcg taatgatcgc
    50821 cagaataaac gccaaaatca gatagacacc atcacgcttc gcgaagaaga acggatcgtt
    50881 ggttaagcgt tgccctatgg gcattgacgc cgaggtcacc atgataaagc caatcgccgc
    50941 gaggccgaag gtcagccaca gtaaggtgcg atcgtacatg atcaggctgt cggtatcttt
    51001 ttcccgcgag cccatcaccc agccctttag cgccgtggag atccagacca ggatactgaa
    51061 tcctggcagg cgcggcattt tcaggcgagg gagagataaa cgcatcaacc taactccttc
    51121 gccagacggg caaactcatt gcctcgttgt tcaaagttct tgaactgatc aaggctggca
    51181 caggccgggg agagcagaac catatcgccc ggctgaacac gcggagccag caagcgcata
    51241 gcctgttcca tagtttcggt ttgttctgcc acttccgggc gtagcgccgc cagctgcgcg
    51301 ccgtcacgac cgaaacaata cagacgtacg ttatcgccat tcaggtaacg caccagtggg
    51361 ctaaagtccg ccgatttacc atcgccgccc agcaacaaat gcagcgtgcc gtctacgtgc
    51421 aggccattca gcgccgcttc cgtactgccg acgttggtcg cttttgaatc gttaatccag
    51481 cgcacaccgt tatgctccag caccacttca aagcgatgcg gcagaccggt gaaggtggtt
    51541 aacgctttca ggctgctggc gcgcggtaac cctgcggcat ctgccagcgc cagcgccacc
    51601 agcgcattgg tgtagttatg ctgcccggaa agtttcatct ctttcacatt cagcactttc
    51661 tcgcctttaa cccgcagcca ggtttcgccc tgctgatgat tcaggtgata gtcacccatg
    51721 ttgacgccaa agctgacgca gcgttcatcc gcaccgcgaa tcggcattgt taaggcatca
    51781 tcagcattaa ccacgcaaac tttcgcgttt tcgtaaatgc gcagttttgc tgcacgatac
    51841 tgttgtaaac caaacgggta gcgatccata tgatcttcag tcacgttcag aatggtcgct
    51901 gctaccgcct gtaagctgga ggtggtttcc agctggaagc tcgacagttc cagcacgtac
    51961 agttcacact catcatccag tagcatcaac gcaggcaggc caatattgcc acccacacca
    52021 acgttaaccc ccgccgcttt cgccatttca cccactagcg tggtgaccgt gcttttgccg
    52081 ttagaaccgg taatcgccac aatcggtgct tgtgcttcgc gacaaaacag ctcgatatcg
    52141 ccaacgattt cgattccggc atcagcggca gcgcttaagg atggatgcgc cagtgcaata
    52201 ccgggactgg cgacaatcag atctgccgcc atcagccatt catcattcag actgcccgtg
    52261 tggcgttcta cggcttcggg taatttatcc aggccaggcg gtgtcatacg cgtatccata
    52321 acgcgcggcg tcacaccgcg agcgaggaaa aagtccacgc aggaaagccc ggtgaggccc
    52381 aggccgataa tgacaacatt tttaccctgg taatcagcca tgattaacgt accttcagcg
    52441 ttgccagacc aatcaggacc agcatcagcg aaataatcca gaaacgcaca atgacgcgcg
    52501 gttccggcca gcctttcagt tcatagtggt gatgaatcgg tgccatgcgg aaaatacgtt
    52561 gtccgcgcag tttaaaggag ccgacctgca ggatgacaga aagcgtttct accacgaaca
    52621 cgccccccat aatcaccagc aggaattcct gacgtagcag tacggcgata atgcctaacg
    52681 caccacctaa cgccagcgaa ccgacatcgc ccataaagac ctgcgccgga taggtgttaa
    52741 accacaggaa gcccagtcct gccccgacta tcgcggtaca gacaataacc agttccccgg
    52801 cgtgtcgcag atacggtatg tgtaaatagc tggcaaagtt catgttgccg gtcgcccacg
    52861 ccaccagcgc aaaaccaccg gcgacaaata cggtcggcat aattgccaga ccgtcaagac
    52921 catcggtcag gtttaccgcg ttgccagtac ccacaatgac gaagtaagcc agcagaatgt
    52981 agaacagccc cagttgcggc atcacatctt taaagaatgg gaccaccagc tgcgttgcgg
    53041 gcgtgtcttt gccggcaagg tacaaagcga aagcaacacc cagcgcaatg accgacatcc
    53101 agaaatattt ccaacgagcg atcaaccctt tggtgtcttt acgcaccact ttgcgataat
    53161 catcaacaaa gccaataaca ccgtaaccta ccagcaccac caacacgcac cagacgtacg
    53221 gattggacgg gtaagcccac agcagcacgg agatcacaat cgctgtcagg atcataatcc
    53281 cgcccatggt cggcgtaccg cgcttgctga agtgtgattc aggaccgtcg ttacgcacta
    53341 cctgaccaaa ggaaagtttt tgcaaatgag caatcatgcg cgggcccatc cacaatgaga
    53401 tgaacagcgc ggtcagcagg ctgacgatgg cgcgaaacgt cagataggaa aagacgttaa
    53461 agccggaata atatttgacc aaatgttcgg ccagccaaac taacatgtcc cattctcctg
    53521 taaagcgcgt actacctctt ccatggcagc actacgtgaa cccttaacta aaatcgtaat
    53581 tacctgttgc tcagcaatca gtgatttaag acgcgtaatt aacgcagttt tatcagcaaa
    53641 atgttcgcca acgccgctgg cggtgctgat agcatggctt tgtttaccca tgcttaacac
    53701 gcggtcaata ccagcagctt ttgccgcctc acccacctgt acatggcagg cttcgctttc
    53761 agcgcccagt tccgccatat cgcccaccac cagcacgcgg tagcccggca tttcagccag
    53821 tacctgaaca gctgcggtca ttgaaccgac attggcgttg taggagtcgt cgagcagcaa
    53881 ctggttttct gccagttgga tggggaacag acggccagga acagctttca gatttgccag
    53941 ccccgctttg atagcatcaa gcgttgcgcc cacggacatg gagagcgcag cggctgccag
    54001 cgcattcgca atattgtgac gccccggcaa cggcagcaga acatcgacgc taccggttgg
    54061 ggtttgtagg gtaaattcca taccgtgcga ggtcacatgg atattggtgg cggtgaaatc
    54121 gctgttggcg gcattgggtg agaaacgcca cactttgcgt gagccaatta cgctctgcca
    54181 gttcagccag tcgttgttgt cggcgttcat aatggcgata ccgttttccg gcaggccgct
    54241 aaagatttca cctttcgctt tcgcgacacc cgcaagcgag ccaaaacctt ccagatgcgc
    54301 cgctgccagg ttgttgacca gcgccgcttc cgggcgagtc agactcacag tccaggcaat
    54361 ctcgccctga tggttcgcgc caagttcaat aactgcgtaa tcgtattccg gcgttaagcg
    54421 caacagcgtc atcggtacac cgatgtcgtt gttgagattg cctgccgtat aaagcgtgtt
    54481 gccgcactgg cttaaaatcg ccgccgtcat ctctttaacg gaggttttgc cggaggaccc
    54541 cgtcagagca accacgagcg ccggaacttg ctggcgaacc catgcagcca gttcaccaaa
    54601 cgccagacgc gtatccttga cgattaactg cggcaggtcg atgtccagcg gacggctaac
    54661 cagtagtgcg cctgcgccgc cagctttcgc ctggtcggca aaatcgtggg catcaaaacg
    54721 ttcacctttc agggcaacaa acaggcagcc cggcgtcagt tttcgggtat cagtggttac
    54781 agcatcaagg gtgatatctg caccttgcag ttcaccgttg agaatatcgg taagttggct
    54841 aagggttacg ctaatcatgc aatccccccc agcagacgcg ccaccgtgac gcgatcggag
    54901 tagtccagac gctgattgcc aacaatctgg taatcctcat ggcctttgcc cgcgaccagt
    54961 accacatcat tctctttagc ctgcataacg gcgcaagtca ccgcttcagc acggccttcc
    55021 atcactttgg catgtccggc atctaacatt cccgccagaa tatcgttgat gatggcacgc
    55081 ggttcttcgg tacgcgggtt atcgtccgtc accaccgcca cgtcagcaaa ctcttcggca
    55141 attgcgccca tcagtggacg cttaccttta tcgcgatcgc caccacagcc aaagacgcac
    55201 cacagcttgc ccgcacagtg caggcgcgcc gcctgtaagg ctttttccag tgcatccggc
    55261 gtatgcgcgt aatccaccac caccgtcggt ttgcctggcg cagtgaacac ttccatacgt
    55321 ccgcaaaccg gttgcagacg cgcggcggtt ttcagcagat cagccagtgg atagcccagc
    55381 gccagcagtg ttgccagcgc gagcaacagg ttgctgacgt taaaagcgcc catcagatgg
    55441 ctttcaattt cgccatcgcc ccagcttgaa ctaaagcgaa tggtcgcgcc gctgtcgtga
    55501 tagttcactt cggtcgcttt caaccagcgt ccgtgacagt tcggattgat atgatcttcc
    55561 attgataccg caaccgcgtc cggcagtttt gccagccagc ggcggcccac ttcatcgtcg
    55621 gcgttaataa tcgcctgacc gcaatgatgt tcagaataaa gcagccattt cgcagcttcg
    55681 tagtgttcca tatcaccatg ataatcaagg tgatcgcggc ttaagttggt aaagaccgac
    55741 gccggaaatt tcaatgccgc cacacggtgc tgtaccagcc cgtgggagga aacttccatt
    55801 gcgcaaaaag tcgcgccctg atccaccagc cccgccagct catgctgaac atcaactgcc
    55861 gaaccggtag tattttctgt cgggatcact ttccccagca gaccgttacc aacggtgccc
    55921 attaccgcgc tggtttcgcc aagcagttgg ctccactgcg ccagaagctg ggtcgtcgtg
    55981 gttttgccgt tggtgcccgt tacgcctacg agacgtaaat tgtcagaggg ttcatggtaa
    56041 aagcggcccg ccagtgcaga taaacgctcg ttgagctggc tgagatagat gaccggtacg
    56101 ccgtgcattt cacggatttc accgtcggtc gcctcatctt tcgcctctgc aataatggca
    56161 gccacacctt gcgctatcgc ctgcgggata tatcgacgcc cgtccgcctg atgacctact
    56221 acagctacaa agagatcgcc cgccgcagcc acacggctgt cgagtgtcat ctctcgcagt
    56281 gctcgcgaag gtgcgtctgg cacccacgga gcaagaaggt cgcgcaaatt acgatctgcc
    56341 acctgtcccc tcgccttgat taatcacaaa ttcattttta tcgcccgttg tcagcgcatc
    56401 cggctcgatg ttcatggtac gcaatacgcc gcccatgatg gcaccaaaga ccggcgcgga
    56461 aacggcgccg ccgtagtatt tacccgcctg cggatcgttg ataacaacaa ccagcgcgaa
    56521 gcgcggctga ctcgcaggcg caacgcctgc ggtataagca atatatttat tgatgtagcg
    56581 accgtccggc ccgacctttt tcgcggtacc ggttttaatg gcgatacgat agcctttaat
    56641 cgccgccttc acgccgccgc cgcctggtag cgccacgctt tccatcatat gcaccacggt
    56701 gcgaacaatg gattccggga agacacgttc accgggaacc ggggggtcaa ctttggtaat
    56761 cgacagtggg cgataaatgc cgtagctgcc gatagttgcg tagactcgcg ctaactgtaa
    56821 cggtgttacc attagcccgt agccgaaaga gaaggtggcc ctctctatgt cagaccaccg
    56881 ttgtttttga ggatataagc cactgcgttc tccgaccaac cccaaattgg tcgcttttcc
    56941 cagtccaaaa cgtgagtaag tatctactaa cgctgaggac ggcatcgtta acgccagctt
    57001 ggaaacaccg acgttactcg acttctgtaa taccccggtc agggttaatt cgctgtagcg
    57061 tgccacgtct ttgatttcgt ggccgttaat tcgataagga atggtattga gtaccgagtt
    57121 ttcccgcacc acgccacgtt gcaacgcggt catcaccacc atcggtttaa ccgttgagcc
    57181 cggttcaaac acgtcggtga tggtacggtt acgcatcgcc tctttcggcg tgccgctcag
    57241 attgttaggg ttgtatgacg ggctgttagc catcgccagc acttcacctg tgttgacatc
    57301 caccagcacg gcgctaccag attcagcctt gttaaaggcc accgcgttgt tcagttcgcg
    57361 ataaaccagc gcctgcaggc gttcatcaat actcagcgcc aggttgtgcg ctgcctggct
    57421 gtcagtagaa gaaatatctt caattacgcg accatagcgg tctttacgca caatgcgctc
    57481 acccggctgc ccggtaagcc atttatcgaa actcttctca acgccctcaa tcccttgact
    57541 atcgacgtta gtaaagccga tgaggtgagc agtcacttcg ccggacggat agtaacggcg
    57601 agactcttca cgcagatgaa tccccggcag tttcagtttt ttgatgtagt ccgccatgtc
    57661 agagtttacc tgacgcgcca gataaataaa gcgccctttc gggttggcgt taatgcgggc
    57721 agaaagctga tccagcggaa tattgagcgc gttagccagc gccttccagc ggtcaccgac
    57781 gctgataccg ccagcgtcat gcacttcttt cgggtcagcc caaatcgctt ttaccggcac
    57841 gctcaccgct aacgggcgac cagaacggtc agtaatcatg ccgcgggagg tggaaacttg
    57901 ctgaacgcga agagaacgca tgtcgccctc tttcaccagc atatccgggg agataacttg
    57961 taaccacgct acgcgtccga gcagaaaagc cagcgccagg agaatacagc cgcataacaa
    58021 cgcaaaacgc caactgataa agttggcatg ttcttcctga cgttttggtt tctgcgtttt
    58081 cgccgctgct ttcatgcgtc gcgtttatcc ttatttttgc actacgatat tttcttgtga
    58141 cggatcaaca tgctgcattt gcagcttttc cgtggcgatc ctttccaccc ggctatggtc
    58201 gccgagcgca ttctcttcaa ggatcaggtt gcgccattca atgtctaaag catctcgctc
    58261 cagcaccagt tgttcgcgct gagcggtcag taaacgggta tggtgcgccg tggttaccac
    58321 ggtcaccgcc gtcaaaataa tgcaaatgaa caggcagagt ggcagcttcc caaatcgcaa
    58381 aagatcgtca ccgataacac caggcaatgc atggcgctcg tggcttccca tcgatccttt
    58441 aactttgctt agagcttctg tcactctgct gatcatgcgt tcgtcctctc tgcaatacgc
    58501 agaactgaac tacgggcacg agggttctca gccacctctt cttcgcccgg cattaacttg
    58561 cctagtgctc gcagctgacg gccacccagt tttttgagct gctcttcagt catcggtaac
    58621 cctgccggaa cttgcggacc gcggctgttt tcacgcataa aacgtttcac aatacggtct
    58681 tccagcgagt ggaagctgat gatcgaaagc cgcccacccg gggccagcac gttgagcgag
    58741 ctttttagcg cctgctctat ctcctccagt tcactgttta cccaaatgcg caccgcctgg
    58801 aaggtacggg tcgcgggatg tttaaactta tctttcaccg gcgttgcagc agccacgact
    58861 tccgccagtt ctttggtgcg ggtcatcggc tgttcgcggt tacgctcgac aatggcgcgg
    58921 gcaatgcgtt tggcaaaatg ctcttcacca taggttttca atacccaggc gatatcggct
    58981 tcttctgcgg tttgtagcca ttcagcggct gactgcccac gggttgggtc catacgcatg
    59041 tccagcggac catcgcgcat aaaggaaaag ccgcgttcag catcatcaag ttgcggtgaa
    59101 gagacgccaa gatcgaggag aatgccgtcg atcttgccaa taagatcgcg ctcggcaacg
    59161 tattcgccca gcgcggagaa aggtccgtgg atgatggaga agcgcggatc atcaatagtc
    59221 ttcgcaacgg cgatagcctg cgggtcgcga tcgatcgcca gcaaacgccc ctcttcgcca
    59281 agctgcgaga ggatcagacg tgagtgacca ccgcgaccaa aagtcccatc aatgtagatg
    59341 ccatcaggac ggatattgag gccattaacg gcttcatcca gcagcaccgt agtatgttta
    59401 tagttttcca tcattttata gagacaagtc ctgcagtcgc tccgataagt ctccggtagc
    59461 caactgctct gcgtcgatat cttccttgac ctgttgatgc caggttgttt catcccacag
    59521 ctcaaacttg ttgaactgtc caaccagcat cacttctttt gtcagcccgg catgttgccg
    59581 cagtactggc gcgattaaca atcgacctgc gccatccatc tgacattcgc tggcatgacc
    59641 taacagtagg cgctgcacac ggcgctcaac cgggttcatg ctcaacagac gcgataattt
    59701 ttgctcgata atttcccatt caggcagggg gtaaagcagc aggcacgggt gatgaatgtc
    59761 aatggtgcaa accatttgac cggcagcgtt ctcaagcagc tgttcccgat aacgggtagg
    59821 cactgataag cgccctttgc tgtcgagatt gactaacgtt gctccccgga acatgccagc
    59881 ctcacccctt attcccactt tgccccacaa tttcccactg aaaggagttt acggagcgga
    59941 ggaaaagctt gtcaagccag tcacaaggca taccgaggta aaaaacccga tgtttacggc
    60001 taatatcagc gttgtttatt ttacgtccaa cgtgcgaagc aaattaacgt tatgaatagc
    60061 tgtaagaaaa aatccctcat cgtcatcact gttttaaaat gactcactat tttccacagc
    60121 aaatataaag tgtcagtttg cgacgcgagc ggcattttag gacatatctt ccccggttaa
    60181 cagtccttgt tacgtctgtg tggcgcttga tgccagcggg tttgcgcaaa agcaaagaaa
    60241 agtgtttgtt aaatcggcaa ttcatcgccg catgtaacaa aataatgcaa aaacgcgctg
    60301 tcgatatcac ttaattattg tgaatttcag ctggtgagtt aattaacggg cgggtttaaa
    60361 accatttatc aataatagtc aggaatagtc ttatttactt taaggatatt gat
//
LOCUS       DAJTSI010000005        60329 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000005 DAJTSI010000000
VERSION     DAJTSI010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.005"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..868)
                     /gene="rpnA"
                     /locus_tag="OO327_000254"
     CDS             complement(<1..868)
                     /gene="rpnA"
                     /locus_tag="OO327_000254"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCR6016935.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAA"
     gene            complement(1071..1307)
                     /gene="feoC"
                     /locus_tag="OO327_000255"
     CDS             complement(1071..1307)
                     /gene="feoC"
                     /locus_tag="OO327_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR6016936.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(1307..3628)
                     /gene="feoB"
                     /locus_tag="OO327_000256"
     CDS             complement(1307..3628)
                     /gene="feoB"
                     /locus_tag="OO327_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR6016937.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3645..3872)
                     /gene="feoA"
                     /locus_tag="OO327_000257"
     CDS             complement(3645..3872)
                     /gene="feoA"
                     /locus_tag="OO327_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR6016938.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(4310..6631)
                     /locus_tag="OO327_000258"
     CDS             complement(4310..6631)
                     /locus_tag="OO327_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR6016939.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDATLLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6728..7204)
                     /gene="greB"
                     /locus_tag="OO327_000259"
     CDS             complement(6728..7204)
                     /gene="greB"
                     /locus_tag="OO327_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR6016940.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            7432..8151
                     /gene="ompR"
                     /locus_tag="OO327_000260"
     CDS             7432..8151
                     /gene="ompR"
                     /locus_tag="OO327_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR6016941.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            8148..9500
                     /gene="envZ"
                     /locus_tag="OO327_000261"
     CDS             8148..9500
                     /gene="envZ"
                     /locus_tag="OO327_000261"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR6016942.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(9576..11198)
                     /gene="pckA"
                     /locus_tag="OO327_000262"
     CDS             complement(9576..11198)
                     /gene="pckA"
                     /locus_tag="OO327_000262"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR6016943.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            11577..13328
                     /locus_tag="OO327_000263"
     CDS             11577..13328
                     /locus_tag="OO327_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR6016944.1"
                     /translation="MDNVELSPATRWGMIATGLLQGMIATGLLQGLVCYLLIAWLAGK
                     NHSWIVYGVPATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNP
                     WRAEKAIWDFGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLS
                     NGLTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDS
                     IQKLFTLIATGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAI
                     VRDPQKASLPWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAEL
                     VLLVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSH
                     MARYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQH
                     LQEQVSEKVFAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDG
                     QAERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKA
                     WRDLTVDGETLEINLSK"
     gene            complement(13472..14341)
                     /gene="hslO"
                     /locus_tag="OO327_000264"
     CDS             complement(13472..14341)
                     /gene="hslO"
                     /locus_tag="OO327_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR6016945.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(14366..14767)
                     /gene="hslR"
                     /locus_tag="OO327_000265"
     CDS             complement(14366..14767)
                     /gene="hslR"
                     /locus_tag="OO327_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR6016946.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14778..15446)
                     /gene="yrfG"
                     /locus_tag="OO327_000266"
     CDS             complement(14778..15446)
                     /gene="yrfG"
                     /locus_tag="OO327_000266"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR6016947.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(15511..17646)
                     /gene="igaA"
                     /locus_tag="OO327_000267"
     CDS             complement(15511..17646)
                     /gene="igaA"
                     /locus_tag="OO327_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR6016948.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            17966..18526
                     /gene="nudE"
                     /locus_tag="OO327_000268"
     CDS             17966..18526
                     /gene="nudE"
                     /locus_tag="OO327_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR6016949.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18691..21243)
                     /gene="mrcA"
                     /locus_tag="OO327_000269"
     CDS             complement(18691..21243)
                     /gene="mrcA"
                     /locus_tag="OO327_000269"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR6016950.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            21363..22142
                     /gene="hofM"
                     /locus_tag="OO327_000270"
     CDS             21363..22142
                     /gene="hofM"
                     /locus_tag="OO327_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR6016951.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            22142..22681
                     /gene="hofN"
                     /locus_tag="OO327_000271"
     CDS             22142..22681
                     /gene="hofN"
                     /locus_tag="OO327_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCR6016952.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            22665..23105
                     /gene="hofO"
                     /locus_tag="OO327_000272"
     CDS             22665..23105
                     /gene="hofO"
                     /locus_tag="OO327_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR6016953.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            23095..23501
                     /gene="hofP"
                     /locus_tag="OO327_000273"
                     /pseudo
     CDS             23095..23501
                     /gene="hofP"
                     /locus_tag="OO327_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            23413..24651
                     /gene="hofQ"
                     /locus_tag="OO327_000274"
     CDS             23413..24651
                     /gene="hofQ"
                     /locus_tag="OO327_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR6016954.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            25052..25573
                     /gene="aroK"
                     /locus_tag="OO327_000275"
     CDS             25052..25573
                     /gene="aroK"
                     /locus_tag="OO327_000275"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR6016955.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            25630..26718
                     /gene="aroB"
                     /locus_tag="OO327_000276"
     CDS             25630..26718
                     /gene="aroB"
                     /locus_tag="OO327_000276"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR6016956.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26810..28102
                     /gene="damX"
                     /locus_tag="OO327_000277"
     CDS             26810..28102
                     /gene="damX"
                     /locus_tag="OO327_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR6016957.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            28209..29045
                     /gene="dam"
                     /locus_tag="OO327_000278"
     CDS             28209..29045
                     /gene="dam"
                     /locus_tag="OO327_000278"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6016958.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            29063..29740
                     /gene="rpe"
                     /locus_tag="OO327_000279"
     CDS             29063..29740
                     /gene="rpe"
                     /locus_tag="OO327_000279"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR6016959.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29733..30491
                     /gene="gph"
                     /locus_tag="OO327_000280"
     CDS             29733..30491
                     /gene="gph"
                     /locus_tag="OO327_000280"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR6016960.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            30484..31488
                     /gene="trpS"
                     /locus_tag="OO327_000281"
     CDS             30484..31488
                     /gene="trpS"
                     /locus_tag="OO327_000281"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR6016961.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            31758..32663
                     /locus_tag="OO327_000282"
     CDS             31758..32663
                     /locus_tag="OO327_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6016962.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            32680..33042
                     /locus_tag="OO327_000283"
     CDS             32680..33042
                     /locus_tag="OO327_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR6016963.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            33126..34289
                     /locus_tag="OO327_000284"
     CDS             33126..34289
                     /locus_tag="OO327_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCR6016964.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            34290..35516
                     /locus_tag="OO327_000285"
     CDS             34290..35516
                     /locus_tag="OO327_000285"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR6016965.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            35513..36391
                     /locus_tag="OO327_000286"
     CDS             35513..36391
                     /locus_tag="OO327_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR6016966.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            36402..36755
                     /locus_tag="OO327_000287"
     CDS             36402..36755
                     /locus_tag="OO327_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR6016967.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            36767..38071
                     /locus_tag="OO327_000288"
     CDS             36767..38071
                     /locus_tag="OO327_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR6016968.1"
                     /translation="MDLYIQIIVVACLTGMTSLLEHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            38083..39168
                     /locus_tag="OO327_000289"
     CDS             38083..39168
                     /locus_tag="OO327_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6016969.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(39328..40059)
                     /gene="phoB"
                     /locus_tag="OO327_000290"
     CDS             complement(39328..40059)
                     /gene="phoB"
                     /locus_tag="OO327_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR6016970.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(40159..40944)
                     /gene="frlD"
                     /locus_tag="OO327_000291"
     CDS             complement(40159..40944)
                     /gene="frlD"
                     /locus_tag="OO327_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR6016971.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(40941..41771)
                     /gene="frlC"
                     /locus_tag="OO327_000292"
     CDS             complement(40941..41771)
                     /gene="frlC"
                     /locus_tag="OO327_000292"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCR6016972.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            complement(41821..42843)
                     /gene="frlB"
                     /locus_tag="OO327_000293"
     CDS             complement(41821..42843)
                     /gene="frlB"
                     /locus_tag="OO327_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR6016973.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(42864..44252)
                     /gene="frlA"
                     /locus_tag="OO327_000294"
                     /pseudo
     CDS             complement(42864..44252)
                     /gene="frlA"
                     /locus_tag="OO327_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            complement(44496..44663)
                     /locus_tag="OO327_000295"
     CDS             complement(44496..44663)
                     /locus_tag="OO327_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR6016974.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            complement(44910..46283)
                     /gene="cobA"
                     /locus_tag="OO327_000296"
     CDS             complement(44910..46283)
                     /gene="cobA"
                     /locus_tag="OO327_000296"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6016975.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            complement(46302..47108)
                     /gene="nirC"
                     /locus_tag="OO327_000297"
     CDS             complement(46302..47108)
                     /gene="nirC"
                     /locus_tag="OO327_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR6016976.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            complement(47234..47560)
                     /gene="nirD"
                     /locus_tag="OO327_000298"
     CDS             complement(47234..47560)
                     /gene="nirD"
                     /locus_tag="OO327_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR6016977.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            complement(47557..50100)
                     /gene="nirB"
                     /locus_tag="OO327_000299"
     CDS             complement(47557..50100)
                     /gene="nirB"
                     /locus_tag="OO327_000299"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR6016978.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            complement(50362..51543)
                     /gene="tsgA"
                     /locus_tag="OO327_000300"
     CDS             complement(50362..51543)
                     /gene="tsgA"
                     /locus_tag="OO327_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR6016979.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGTIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            51814..52395
                     /gene="ppiA"
                     /locus_tag="OO327_000301"
                     /gene_synonym="rotA"
     CDS             51814..52395
                     /gene="ppiA"
                     /locus_tag="OO327_000301"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR6016980.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            52500..52667
                     /locus_tag="OO327_000302"
     CDS             52500..52667
                     /locus_tag="OO327_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR6016981.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            52657..53259
                     /locus_tag="OO327_000303"
                     /pseudo
     CDS             52657..53259
                     /locus_tag="OO327_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
     gene            53291..53854
                     /gene="pabA"
                     /locus_tag="OO327_000304"
     CDS             53291..53854
                     /gene="pabA"
                     /locus_tag="OO327_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR6016982.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            53940..55160
                     /gene="argD/dapC"
                     /locus_tag="OO327_000305"
     CDS             53940..55160
                     /gene="argD/dapC"
                     /locus_tag="OO327_000305"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR6016983.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(55227..57317)
                     /locus_tag="OO327_000306"
     CDS             complement(55227..57317)
                     /locus_tag="OO327_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR6016984.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(57368..58000)
                     /gene="crp"
                     /locus_tag="OO327_000307"
     CDS             complement(57368..58000)
                     /gene="crp"
                     /locus_tag="OO327_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR6016985.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            58302..58706
                     /locus_tag="OO327_000308"
     CDS             58302..58706
                     /locus_tag="OO327_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR6016986.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            complement(58761..59630)
                     /locus_tag="OO327_000309"
     CDS             complement(58761..59630)
                     /locus_tag="OO327_000309"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR6016987.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(59684..59902)
                     /locus_tag="OO327_000310"
     CDS             complement(59684..59902)
                     /locus_tag="OO327_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR6016988.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            complement(59896..>60329)
                     /locus_tag="OO327_000311"
     CDS             complement(59896..>60329)
                     /locus_tag="OO327_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR6016989.1"
                     /translation="VYQRYLLNLLKANAARKLAAYPGTLPINLAQLKSVRRIREFDDL
                     ITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIHAKDDPFMDHQVIPKPESLPSQVE
                     YQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTYLEAKSC"
BASE COUNT        14172 a        15344 c        16326 g        14487 t
ORIGIN      
        1 ccgcagccaa ctcttcttct gacagcccgg taaatcgcat gatgtctgca agagggactc
       61 cggattcaag cattattttg gctatatgca gggctttgga ttgttccccc tcctgccgca
      121 atctttccgc aatagtcatt aaactctcct tgtgtttcgg tgaacgttcg gcaacgccgt
      181 cgataaaatc gttaaaacgt acagcgtcgc cagtttgcag tatgtaatta aacagccctt
      241 tgatttgtct gtcattagcg tatccactac ttaataagca ggccatttgc tctaccagcc
      301 ccatcagatc gcgttgacga atatgttttt gaattaactc cagcagcgcc atacgtcggt
      361 gctgcatgat ttcatcatca ggcatgacgg tgacatcaat cagcggaaat gcggaggcat
      421 aaagctgcct tgccagtttg ggatcggcga aacaatccag ccaacacagc gaatagggat
      481 aggggctttc aataccgtgg taaaacaaca atggcaccac catcggcaac gttttgtatc
      541 cagcatcaag gtgattttgc attgcggcaa tagcgtaacg catcatgcga aatgcgatca
      601 gtttgtttga ggtgctttga tgttcaatca gacaatagat gtatcctggt ccttgttccg
      661 ttttcaccga ccacagcaca tcggaatagc tttcacgcag atcgtcatca ataaagctcc
      721 tcgactccag cttgagggtt ttcatatcac agagcgcgtg aatgggtgcc ggtaaatgaa
      781 acgcaagaaa atcgcgtgcc gtctccggtt ggcgtaaaaa gagtttgaac agcgcatcat
      841 gtggggtgga actctgcttt ttactcatgg cgttccttca cctgttgttt gaatgccgcg
      901 actctacgcg ccactccaca tctgtgcatt agcgagtttt catccctgcg agacgctttc
      961 agaggaactt acgttgcgct gcaagaattg catttttatg gaaagccgca cgcaaaaaag
     1021 cccggatgca aacgcatacc gggcttttca aaacaggcga tggagtatgg ttaacgcagc
     1081 gcccaccact cgcgcagaca ggctttgcct tccgggcagc ttttacaact gccagagagg
     1141 cagccgtcag gttcttcctg aatccgcacg gctttgccca tgctttccag ctgttgcagc
     1201 atggcgttaa tcattggctg cggagtgttc aatgtctggc ttatctgggt cgcttccata
     1261 cggccccgta acgccagcaa atcacgtacc tgaataagtg aagccattaa tggcaatcac
     1321 cggtggtgct ggctgcgcag caactgctta ccgacttgcg ggttgccagc agttcgatat
     1381 ccacccggct acgcgcgcgg cgcagcagac cgatagctac gatgttaaac aggattaccg
     1441 ccagaatgca caccaggctg taagttgggt gctgactgta gctggcgacc tgatagaaca
     1501 atgttgccag tgagtaagcg atattcagcc cccacaggat ggagaagccc atccagccac
     1561 ggcttgattc acgggcgatg gctcccatca ccgagataca tggtacatac agcaggacga
     1621 aaatcaggta gctgtatgcg gcagctgcgc taccgaattt ctgatccatc acgcccatcg
     1681 ccccggtacc catttcaccg ttgcctttgc tagcttcaat ggggttcatc agtacgctaa
     1741 ggctgaaggt gtctttcagg ctctgccagg tttcatctac cgcactgaac agctcttcac
     1801 cgaggttgaa ttccgccgga ttgaactctt cgtcctgaat attttctgcg gtgtagaggg
     1861 tgttgagcgt acccaccacc acttcttttg ccattgcgcc cgtaaacagg ccaaccgttg
     1921 cctgccagtt atcttcatgt acgccaatcg gcttgaagac aggggttatc acccggctga
     1981 cggaagccag cgccgagtcg ttgatgttat cgacgatttt cccgctcaac gagaagctgt
     2041 tgaaagcgct caggaaaatg ctgacgatga taatcacttt accagcacgc agaacgaagc
     2101 ccttcagacg ctgccaggtc tggataatca ggcttttaac gtgtggtaca tggtagaccg
     2161 gcagctccat gacaaacggc gtcgcttcac cgcgcatgat ggtgtacttg agcatcaggc
     2221 cagtcagcac cgccatcacg ataccgagca tatacagcga gaaaaccgcc agggcaccgt
     2281 tctgcccgaa gaaggcagcc gcgaatactg cgaagatagc cagacgcgcg ccgcaggaca
     2341 taaacggtgc catcatgatg gtcatcagac gttcacgcgg tgcatcaagc gtacgtgcgc
     2401 ccattaccga cggtacgtta caaccgaaac cgacgatcag cggcacaaag gatttccccg
     2461 gcaagcccag cgcctgcatc aggcggtcca tcacaaacgc tgcacgcgcc atatagccgg
     2521 agtcctcaag gaaggagagg aacaggtaca tcatgccgat ctgcggcacc agtggcagca
     2581 cggtgttaat accgccaccc aggccctggg cgaggaagat agtcagccag tccgggaagt
     2641 ggagcgtgta gccaatccat tgaataccat gcacaaatag cgccacggag ccgacgttaa
     2701 acagcggctg taacgccccg ccgatgttga tagccagcag gaacatcagg tacatcacaa
     2761 agaggaaaat cggcagaccg aggaaacggt tgagcacgat tttatctacc gcagtggtga
     2821 aacggctggg ttctgccgtc agggtgttgc ttaccacatc acagatggca gcaatgcact
     2881 ggtaacgcgc atcggcaatg tgcagcgccg gatcatccat ttcattacgc agacgggcga
     2941 gggtggcatc cagatgctgc gacgcttcac cggcgtaggc gcggctgtag atatcgcctt
     3001 ccagcatttg caggcccagc cagcgacgtt gtttcagcgg gatgtcggaa ggcatcactt
     3061 ttgccagtga atctgcttcg ttgagtagcg gctgtgcgta atgcaccagt tccacattct
     3121 cgttagcttt atagcgatca atcgccagct tgagcgcttc aataccgcga ccacgggttg
     3181 aaaccagcgg gatcaccggg cagccaagac gtgccgatag agcatcaatt tcaatacgaa
     3241 tattttgctt ctcggcaatg tcgagcatgt tcagcgccac gatgcaggga atgccgagtt
     3301 ccagcagttg cagcgtcagg tacaggttac gctcaaggtt agacgcatcc accacgttaa
     3361 tcagcaggtc ggcgtcgcca ctcaaaatgt agtgacaggc gatttgctca tcgagcgagg
     3421 tctgcgatga gatggtggtc agagaatagg tgccgggcag gtccaccagc gtgacctgat
     3481 gatcggtggt ggagaattgc ccttctttac gttcgacggt aacgccagcc cagttaccta
     3541 cacgctgacg tgcgccagtg agctggttaa ataaggttgt cttgccagaa tttggattac
     3601 caattaagcc aatggttaat tttttcattg ttgttatcac cgtattaaca ggaaaccgct
     3661 tccacttcta ataaggccag atcttttttg cgtaatacca ggctcacacg acgggtttcg
     3721 atatgaatgg ggtcgccgag tggagcgacg cgcaccacat caaaagagga gccaggtaac
     3781 atgcctagag aaagcagttt ttggcgatat gccgggctga tttcacggga aaagccagtg
     3841 attttccacg cagtatctgg agtgtattgc ataggtgcct acttgtttct cattaactgg
     3901 ataactacct catggggcgt ttgaccgcga catgagacca gatgatttgc caacaaagga
     3961 gagattaaat aatcaacaac acaatgataa tgagaatggt ttctatcagc aatacttaaa
     4021 atgtgtgcga atgttggttt gaataataat ctgactcaat gttgatgtgg ctcaaggtta
     4081 tatcgcagcg aaaatataat tcgaatagtg agttacccgg actggctaat gtttaattaa
     4141 ggtttcagtt gttaatgaag tgataaatat taacggagta tattgtgagt ttggctgtgg
     4201 aataaaagtg ccggagatat tctccggcac ttttatgtca ggcaaagcgt tcgttgccag
     4261 atgcggcgtg aacgccttat cgcgcaggtt taggggccag tccgggcgtt taacgttttt
     4321 tacccatcgc tgccgccagc gcatccatca tcgcgctatt accggcaggc tgcgcttcac
     4381 gaccgcgtgg tttggctgcc gggcggttgt tttgcgggcg ttcattaccg ccgccgcgac
     4441 gagagttggt ttcgccaggc tgctcgtcca gacgcatagt cagggcgata cgtttacgct
     4501 gaagatccac ttccagcact ttcaccttca caatgtcgcc cgctttcacc acggtatgcg
     4561 gatcttccac aaacttgttc gacaatgaag agatgtgaac caggccgtcc tgatgcacgc
     4621 caatatcgac aaacgcgcca aagttggtga cgttggtcac tgcgccttcg aggatcatac
     4681 ccggttgcag gtcgttcatt gtctcgacgc catcggcaaa ctgagcggtt ttaaattccg
     4741 gacgcggatc gcgacccggt ttttccagct ctttgatgat gtcagttact gtcggcacac
     4801 cgaatttttc atcagtaaag tcagacgctt tcaggttacg cagttcgctg ctgttaccca
     4861 tcagatcttt cagtgcctgc tgtgttgctg ccagaatgcg ttccaccacc ggataggctt
     4921 ccggatgaac ggtagaggcg tccagcgggt tatcaccgtg gttaatgcgc aggaagcccg
     4981 cgcactgctc gaaggctttc ggccccagac ggctgacttt cagcagttgc tgacggttct
     5041 ggaactggcc gttctcatcg cgccaggcca cgatgttttg cgccatcatg cgcgtcaggc
     5101 ccgccacgcg ggttaatagc ggaacagaag cggtgttgag atcgacgcca acggcgttta
     5161 cgcagtcttc tactactgca tccagtttgc gggccagttg cgtctggctg acgtcatgct
     5221 gatactgacc tacgccgata gatttcggat cgattttcac cagctccgcc agcggatcct
     5281 gcaaacggcg ggcgatagat accgcaccac gcagcgaaac gtcgagatcc gggaactcct
     5341 gcgctgccag ctcggaagct gagtaaaccg acgcgccagc ttcgctgacg atcactttct
     5401 gtgcggtcac tttcgggaac tgcttctgca cgtcgagata gaaacgttca gtttcgcggg
     5461 aagctgtacc gttgccgatc gccaccagtt caacgttatg tttttcacac aaggcagcaa
     5521 cggtcatcgc tgcttttgcg gcttgtccgg tgtgcgggta aatggtatcg gtcgccacca
     5581 gtttgccagt ggcatcgacc accgccactt ttaccccggt acgcagaccc ggatcgaggc
     5641 ccatcgttgc acgcagtccg gcaggggccg ccatcagcag atcgtgcagg ttacgggcaa
     5701 agacgttgat tgcttcatct tccgcgcgtt cgcgcacggt acccatcagt tcggtttcca
     5761 gatgcatcag caccttgatg cgccaggtcc agctcactac gcttttgcgc cagctatccg
     5821 ccggggcatt gttcaggcgc aggccaaggt gatccatgat gatttgctcg caatagctct
     5881 ctttgggcgg ctcatcgaac tgcggatcgg cattcagcga aagctggagt acgccttcgt
     5941 tacgcccacg gaacatcgcc agcgcgcggt gagaaggcac cgtggacaac ggttcgtgat
     6001 gatcgaaata gtcgcggaat ttcgcccctt cctcttcttt accgctcacc accgtagaaa
     6061 ccaaatgcgc gttcttccac agataatcac gcactttcgc cagcagcgtg gcatcttcgg
     6121 caaaccgttc catcaggata tagcgcgcgc catccagcgc ggctttggta tctgccacgc
     6181 ctttatcggc atcaacatat tgtgcagcgg cgacttctgg cgtgtgtgac ggatcgctcc
     6241 acagcaggtc agccaacggc tcaagccctg cttcaatggc gatttgcccg cgggtgcggc
     6301 gtttaggttt gtagggcagg tagaggtctt cgagttcggt tttgcttagg gtggcgttga
     6361 tggccttcgc cagatcatcg gtgagtttgc cttgctcgga aatggatttg aggatcgcct
     6421 gacgtctctc ttccagctcg cgcagatagc tcagacgcgt ttccagatta cgcagctgcg
     6481 tgtcatccag accgccggtg atttccttac gataacgtgc gataaacggc acggtattcc
     6541 cttcgtcaag caggcgaacg gcagcgtcaa cctgttccgg gcgcgcctga atttcacccg
     6601 caataatgcg gcagaacgaa tcattcatca tggtttgagt tcatcttttc ggatcaaaaa
     6661 tcaggggata gttatacgga ctggctggca aaaatgccag ccatcggcag gaggttaaga
     6721 ctcttcctta cggtttcacg tactcgatag cattaacata ccagttcgct tccccggcag
     6781 gggtattcac caccgccaga tcgccgactt cttttttcag caatgcgcgg gccatcgggg
     6841 aatcgataga gatgtaatct ttacggccaa aaatttcatc gtagccgaca atacggaaac
     6901 ggtgagtcac gccatcgtcg ttttcaatct ccacccacgc gccaaaaaag actttgcctt
     6961 cctgctgagg ggagtaatcg acgattttga gattttccag gcatttagtg agatagcgca
     7021 cgcgacggtc gatttcacgc agacgctttt tattatactg atagtcagca ttttcgctgc
     7081 ggtcgcccag acttgcggcc caggtcacct tttttgtgac ctccgggcgt tcttcacgcc
     7141 agagataatt aagctcttgt ttgagttttt catacccttc ccgggtaacc aggggcgttt
     7201 tcatctcgtt gattcccttt gtctgtttga taatgcgcac attgggtata acgtgatcat
     7261 atcaacagaa tcaataatgt ttcgccgaat aaattgtata cttaagctgc tgtttaatat
     7321 gctttgtaac aatttaggct gaaattcata ccagatttag ctggtgacga acgtgagctt
     7381 ttttaagaat acacgcttac aaattgttgc gaacctttgg gagtacaaac aatgcaagag
     7441 aactacaaga ttctggtggt cgatgacgac atgcgcctgc gtgcgctgct ggaacgttat
     7501 ctcaccgaac aaggcttcca ggttcgaagc gtcgctaatg cagaacagat ggatcgcctg
     7561 ctgactcgtg aatctttcca tcttatggta ctggatttaa tgttacctgg tgaagatggc
     7621 ttgtcgattt gccgacgtct tcgtagtcag agcaacccga tgccgatcat tatggtgacg
     7681 gcgaaagggg aagaagtgga ccgtatcgta ggcctggaga ttggcgctga cgactacatt
     7741 ccaaaaccgt ttaacccgcg tgaactgctg gcccgtattc gtgcggtgct gcgtcgtcag
     7801 gcgaacgaac tgccaggcgc accgtcacag gaagaggcgg taattgcttt cggtaagttc
     7861 aaacttaacc tcggtacgcg cgaaatgttc cgcgaagatg agccgatgcc gctcaccagc
     7921 ggtgagtttg cggtactgaa ggcactggtc agccatccgc gtgagccgct ctcccgcgat
     7981 aagctgatga accttgcccg tggtcgtgaa tattccgcaa tggaacgctc catcgacgtg
     8041 cagatttcgc gtctgcgccg catggtagaa gaagatccag cgcatccgcg ttacattcag
     8101 accgtttggg gcctgggcta cgtctttgta ccggacggct ctaaagcatg aggcgattgc
     8161 gcttctcgcc acgaagttca tttgcccgta cgttattgct catcgtcacc ttgctgttcg
     8221 ccagcctggt gacgacttat ctggtggtgc tgaacttcgc gattttgccg agcctccagc
     8281 agtttaataa agtcctcgcg tacgaagtgc gtatgttgat gaccgacaaa ctgcaactgg
     8341 aggacggcac gcagttggtt gtgcctcccg ctttccgtcg ggagatctac cgtgagctgg
     8401 ggatctctct ctactccaac gaggctgccg aagaggcagg tctgcgttgg gcgcaacact
     8461 atgaattctt aagccatcag atggcacagc aactgggcgg cccgacggaa gtgcgcgttg
     8521 aggtcaacaa aagttcgcct gtcgtctggc tgaaaacctg gctgtcgccc aatatctggg
     8581 tacgcgtgcc gctgaccgaa attcatcagg gcgatttctc tccgctgttc cgctatacgc
     8641 tggcgattat gctattggcg ataggcgggg cgtggctgtt tattcgtatc cagaaccgac
     8701 cgttggtcga tctcgaacac gcagccttgc aggttggtaa agggattatt ccgccgccgc
     8761 tgcgtgaata tggcgcttcg gaggtgcgtt ccgttacccg tgcctttaac catatggcgg
     8821 ctggtgttaa gcaactggcg gatgaccgca cgctgctgat ggcgggggta agtcacgact
     8881 tgcgcacgcc gctgacgcgt attcgcctgg cgactgagat gatgagcgag caggatggct
     8941 atctggcaga atcgatcaat aaagatatcg aagagtgcaa cgccatcatt gagcagttta
     9001 tcgactacct gcgcaccggg caggagatgc cgatggaaat ggcggatctt aatgcagtgc
     9061 tcggtgaggt gattgctgcc gaaagtggct atgagcggga aattgaaacc gcgctttacc
     9121 ccggcagcat tgaagtgaaa atgcacccgc tgtcgatcaa acgcgcggtg gcgaatatgg
     9181 tggtcaacgc cgcccgttat ggcaatggct ggatcaaagt cagcagcgga acggagccga
     9241 atcgcgcctg gttccaggtg gaagatgacg gtccgggaat tgcgccggaa caacgtaagc
     9301 acctgttcca gccgtttgtc cgcggcgaca gtgcgcgcac cattagcggc acgggattag
     9361 ggctggcaat tgtgcagcgt atcgtggata accataacgg gatgctggag cttggcacca
     9421 gcgagcgggg cgggctttcc attcgcgcct ggctgccagt gccggtaacg cgggcgcagg
     9481 gcacgacaaa agaagggtaa ataaacggga ggcgaaggtg cctcccgttt tgctttctat
     9541 aagatactgg atagatattc tccagcttca aatcattaca gtttcggacc agccgctacc
     9601 agcgcggcac ccgcaggggt gtcggtgtat ttatcgaagt tgtcgataaa cagtttcgcc
     9661 agggtttcgg ctttttcctg ccactgttcc ggagaagcgt aggtgttacg cggatcgaga
     9721 atcttcgtgt ctacgcccgg cagttcggtt gggatcgcca ggttaaacat cggcagagtg
     9781 aaggtttctg cattatccag cgaaccgttg aggatggcgt cgataatggc gcgggtatct
     9841 ttaatcgaga tacgtttgcc agtgccgttc cagccagtgt taaccagata agcctgcgcg
     9901 cccgccgcct gcatacgttt caccagcact tctgcgtact gagtcgggtg cagcgacagg
     9961 aacgccgcgc cgaagcaagc ggagaaggtt ggcgtcggtt cggtgatgcc acgctcagta
    10021 ccggccagtt tggcggtgaa gccagagagg aagtgatact gggtttgatc ggcagtcagg
    10081 cgagaaaccg gcggcaacac gccgaaagca tcagcagtca ggaagataac cttagtcgcg
    10141 tggcccgctt tggaaaccgg cttaacgatg ttatcgatgt gatagattgg ataagaaacg
    10201 cgggtgttct cggtttttga accatcatca aagtcgatag tgccatcttc acgcacggtg
    10261 acgttttcca gcagcgcatc acgacggata gcgttgtaga tttcaggttc cgcttctttc
    10321 gacagcttga tagtttttgc gtagcagccg ccttcgaaat taaacacgcc gtcatcgtcc
    10381 cagccgtgtt cgtcatcgcc aatcaggcgg cgtttcgggt cggtggaaag ggtggtttta
    10441 ccggtgccgg aaaggccgaa gaacaccgca acatcgcctt tctcgccaac gttggcggag
    10501 cagtgcatag aagcgatacc tttcagcggc agcaggtagt tcatcatcga gaacatccct
    10561 ttcttcattt cgccgccgta ccaggtgccg ccaatcagct gcatgcgctc ggtcaggtta
    10621 aacgccacga agttttcgga gttcagaccc tgttctttcc actgcgggtt agtgcacttc
    10681 gcgccgttca taacgataaa gtctggtttg aaacctgcca gctcttcatc gctcgggcga
    10741 ataaacatgt ttttgacaaa atgcgcctgc caggccactt cggtgatgaa acggacggaa
    10801 agacgagtat ccgggttcgc accacagaaa gcgtcgacaa cgaacagacg tttgccggaa
    10861 agctgcctgg tcaccaggcc tttcagatgc tgccaggttt ccggagagag aggtttgttg
    10921 tcgttcttac ctttgccttt gtctgcccac cagaaagtat cgcgagtggt atcgtcacgg
    10981 acgatatact tatcttttgg tgaacgaccg gtgaagatcc cggtatcgac ggcaacggca
    11041 cccagattag ttaacacccc gcgctcataa cctgtcaggc tcggatcgag ctcttcctga
    11101 tacagcaggt cgtagcttgg gttgtaaacg atatcatgta cgtcactgat accataagcc
    11161 tcgagttctt gcgggggcaa accattgtta acgcgcattt cactgctcct tagccaatat
    11221 gtattgcctg aatagtaaag tctttttggg ggtgttaacc gcgacaaggc tcatagattt
    11281 acgtatctgg agaaattcat atttgacaaa aaactccgtg attcctgtca cgaaacggtt
    11341 gctattatcg cagaaatagc attttaggtg tggaaaatgt tctcaaaagg ttaaatcttg
    11401 gtttatatgg cgggaatatt gtgagtggaa tcgcattcct gaatgaaatt gatgcaaatt
    11461 caaccggtaa agtatcgatt caccacgcta actttttttg attattttct gaaaaaagca
    11521 aaatactact gcccggatgc gataattaac ccaccttttg attctggata accaacatgg
    11581 ataacgttga actttcaccg gcaacacgct gggggatgat tgctaccgga ttacttcagg
    11641 ggatgattgc taccggatta cttcaggggc tggtttgcta cctgcttatc gcctggcttg
    11701 ccggaaaaaa tcacagttgg attgtttatg gcgtgcctgc gaccgtcgcg ttttcatccg
    11761 ttttactctt ctccgtgatc tcttttaaac agaagcgcct ttggggatgg ctggcgctgg
    11821 tgtttattgc cacgacaggg atgagcggtt ggctgaagtg gcaaactgat ggcatgaacc
    11881 cctggagagc tgaaaaggcg atttgggatt ttggctgcta tctgctgttg atggcaatgt
    11941 tgttgctacc gtggatacaa caaagccttc gtatccgcaa tggcagtagc cgctatagct
    12001 acttttacca gtcagtatgg cataacgtac ttatattact ggtgattttt ctctccaatg
    12061 gcctgacgtg gctggtgctt ttactgtggg gtgagttgtt taaacttgtt ggcatcacgt
    12121 ttttcaatac gctctttttt gcaaccgact ggtttatgta tcttacattt ggtctggtta
    12181 ccgcactggc ggtgatcctc gcgcgtacac agtcacgttt aatcgactct attcaaaagt
    12241 tgttcacgct aatcgccacg gggttgctgc cgttagtatc attgctaacc ctgatgttta
    12301 tcatcaccct gccgtttacg ggcctgagcg cgatttctcg ccacatctcc gccgccgggt
    12361 tgctgttaac gctggccttt ttgcaattga tcttaatggc tattgtccgc gatccgcaaa
    12421 aagcgtcact tccctggacg gggccgttgc gttgcctgat taaaaccgct ttgctggttg
    12481 ctccgctgta tgtgttcgtc gccacctggg ggttatggct gcgggtcgct cagtacggct
    12541 ggactgtcga ccgcttgcag ggcgcgctgg cggagctggt gttactggtg tggtcgctgg
    12601 ggtattttgt cagtatcgtc tggcgtaatg ggcaaaatcc ccttgttctt cagggcaaag
    12661 tgaaccttgc ggtttcgtta ttggtgttgg tgatactggt gcttcttaat tcgccggtgc
    12721 tggacagtat gcgtattagc gtgaacagtc atatggcgcg ttatcagagc ggcaaaaaca
    12781 cgccagacca ggtaactatc tacatgctcg aacagagcgg tcgctatgga cgtgcggcgc
    12841 ttgagtcgct gaaaagcgat gccgggttta tgaaagaccc gaaacgcgcg cgggatctgc
    12901 tgatggcgtt agatggagag caacatcttc aggaacaggt atcggaaaaa gtattcgccg
    12961 aaaatgtgtt aattgcccct ggttctgtta aaccggatgc gacattctgg tcggccttaa
    13021 tccaggatcg ctataacgtg atgacctgta ttgaaaaaga cgcctgcgta ctggtcgagc
    13081 aagatctgaa tagtgatggt caggcggagc ggatcctgtt tgcttttaat gatgacagag
    13141 tcattgtcta tggctttgac tcagacagaa aagaatggga cgcgcttgat atgagtttac
    13201 ttccgaacga aataacgaaa gaaaaattac tcacagctgc gaaggatgga aaactgggga
    13261 cgaggccgaa agcgtggcgc gatctgacgg tagatggtga aacgctggaa ataaacttaa
    13321 gtaagtgaca acgcttttat cggatacgac gctgtatctg accaaaataa tttgcgattt
    13381 atttcatagg cgctggtaag cgcagcacat caggccatta agtcgtaaaa tccggcagag
    13441 ccctctctgc cggacatact cattaatgaa cttacggatc tgccggagac gcgttgttgc
    13501 ggatttcagc aatatccatc gcattgaaca gatagtggtt accgcagtaa tcacaatgca
    13561 tgtcaatttc gccatcttcc gccaggatgc tatcaacttc ttcatcaggc agcgttttca
    13621 gcgcatcggc gcaacgttca cgcgagcagg tgcatttgaa ctccacatcc tgcggatcgt
    13681 aaaccgtcac ctcttcttcg tgatacaaac gccacaacac ttcgtttgcc ggtaaggtca
    13741 gcagttcttc ggttttgatg gtttcggtta gcgtcgccag gtggtcaaag tcgtcctgtt
    13801 gggtattttg cgcaggcatt acctgcaaca acataccgcc tgcagccggt ttgccgtcta
    13861 cgtcgccggt gcgaataaac aggcgcgtcg gcagctgttc agaacgcata aagtaatctt
    13921 ctaggcaggc cgccagggta tcaccttcca gaccaactac gccctgatag cgttcgcctt
    13981 cgctcggggt aatggtgatc accacgtaac cattgccgac cagcgttttc aggtcggcat
    14041 tttctggaat ttcgccctgc acgcgcgcca caccgcgcat ttgctggtta ttgttaccgt
    14101 taataaccgc cagattcatc ggaccgtcgc cctgcagctg tacggtgata tcaccatcaa
    14161 acttcagcgt agcggttaac aggctggtcg caaccagcag ttctgccagc acgtttttaa
    14221 cgggctgcgg ataatcgtgg ttctcaagga tctgttgcag ggtttccgaa acggttacca
    14281 gttcgccgcg aacggcaaaa ttttcaaaca gatagcgatg taattggtca tgttgcggca
    14341 taatcatctc tcttgcaggt gacagttatt cactgtcgcc gtgtttaaat cgtaacaggt
    14401 cgcggcgctc ttttttgtcc gggcgtcggt ccgggtgcgg catggttaag gcattaagtt
    14461 tacgtgccat cgccattttt tcgcgtttct ctacactttc cgcagtctct tcatacagca
    14521 aggctgcctc gctggcgggg cgacgctgtt cagtaatcgc ctttacaatc accgtgcgtt
    14581 cgtcatttcc ctggcgcaga gtgagcgtgg cattcagctc gacgattttg ctcggcttgc
    14641 tgcgctgccc gttgtaatgc accttaccgc cttcaatcat ttcacgggcc agcgcgcggg
    14701 ttttataaaa acgggcagcc catagccatt tatccagtcg aacctcaaca gcaggtttct
    14761 ctttcatggc gtctccttca cattagcgag gggatcaggc ggcggtagtc attcagtgac
    14821 ggatggcgtt gatactgttt ctcggcaatc ccggaatcag gattagtcac gccgaggcag
    14881 taacgaatac caaattgcgc ggcagcatcg agaatcgctt cgctgtcatc aataaacagc
    14941 gttctttcag ctttcagacc cgtagcttcg gccaccgcat gccataaccg ctgatcctct
    15001 ttcggataac caaatgtgtg ggtggaaagt aataaatcaa ggtgtgcgtc cagacccgta
    15061 tgctcaagtt ttaccgccag gttgtgcgga tgagcattgg tgagcaaaat tcgctgctta
    15121 ccgctggctt tcagtgcctc aagaaacgga atggtatctt cacgcagtac ggcgcgcggt
    15181 cccatctcgg tggtcatcgc acagatatcc agacccagtt gctcactcca gtaatcaaga
    15241 cagtaccagt ttagcgtatg ctgtacgtcg tgatattgct ggcgcatata ttccatcgct
    15301 tcctgtggcg taaccccgtt tttcgcgccc catgtttcag gcaccagctt ttgccagaaa
    15361 tagttatcga aggcgaggtc gagcaacgtg ccgtccatat ccagcagaac ggtatctacg
    15421 tcctgccagg caatgttgat atgcatgagg gaaatctcca gagtgaagca atttgcgcga
    15481 cagggtagca taacctgccg cgcaaacgtg ttattcgata aggctttctg aaggggtgat
    15541 cagttgcggg ttcaggcagc tttcataata ggcctgaatc tccatcatgc gagtgcgatg
    15601 acgctggtaa cgacgccagg cctgtacgcc attgtaaatg gcgctaccca gcatcgtcag
    15661 tagcagcaat gtggtgctga gatagcgcca caggccggaa cgatccggga tcggatgaag
    15721 gccaatatgc tgcgtaccat tggcgtcagt gaagattttt gtgacgatac cttcggcgtt
    15781 aaacggcgta tgcatcagca tttgtgccag tttctggaaa gcgttccact gttcttgcgg
    15841 cgggtagtcg taaagtgatg ccgaaggcca gggctgatca acaaaatcgc tgccttcgtc
    15901 gctgacaatc aggaatccgc cgggggccgg actgtttagt gattgtgccg ctcgcgccgt
    15961 ttcatgcgtg ataaacggcg cggtggaggt tgccaccagg ttatccagcg attcagcact
    16021 caccgggcgt aataacacat tcacgccatc gagcttcccg gcgttggcgc gttttaccag
    16081 cgcgtcccag tctttactgt tgccgagatt gaccagcgca tttttcagtc gcacacagtc
    16141 atctttggca gaacataaat ccgctgtctt cagtacaatg tcgccaaaat catcaagcaa
    16201 taccatgccg gatttttgaa tcgctgaacg taatgaggca ctgacgcgag attcatcttc
    16261 cggtttaggg tgcagctggc gattaactgc ttcagtcaat gccgtcgctt tgttgaccag
    16321 ttcagattct ggtaatggta atgagcgggc gtcattccag atgatctgcg agcagtcaaa
    16381 cggtaaaaaa ggtgaattgg ttttcgcgct ccaggttccg gaagtgcgaa tattacacat
    16441 tcccgtaccg ctaatacgca atgtatcgcc tacccgcacg ccagcgtcag ccagttgttt
    16501 tacgctggtg gcttcaatgg tctgcgcgcc tttcatccat gagagagtga atttcagcgg
    16561 catatccagc gggatccaga ataacagcat aaacagcact agcagcgagc ccgcagcgat
    16621 aatcgtactg cgcagccagt gctgtaacgg aaagtttttt acttcatcat gcagcgaaag
    16681 atatcgcccc tgacgcacta cgtggcggtc gagatagata tcaatatcgg tttgttgacc
    16741 gagatcctga gcaatgtatg gctgccagtg tgcgggatag accaggtcga taataccgag
    16801 tgaaatattg ttgatctgtt cctgatcgtt ttcgccaaac aatccccaac gacggggtgt
    16861 accgcgcaga caatgaattt ctcgcaggga ggattttgcc gggggcgcga atagccccca
    16921 cagacctgcg cccagcagca gtaatgcgcc gcctgccagc cacggaacaa atacatccgg
    16981 ggtaatcagg cagaaaaaga acatcaggaa ggaggcgacg atcagcaacg cttcacgcag
    17041 cccgcgcgga cgactcagcg catattcttc atgcgtttct ttgcgaatat tgagtagctc
    17101 gatttgctca ctctcttccc cgcgaatcga cgcctgcgtg gacggaacag gttgcaaggc
    17161 atagccgcgc gtttcctgca tgtactcctg cagcgtatga ccgttgagtg aaataaccaa
    17221 cggcagcgaa tcggtatgaa tcagttcaac ggtattctca tcgttgatgt actgttccca
    17281 gaaggggggc aggtggactt ctaccgaatc gaggtagtaa cgccatttat tcggatcgtc
    17341 ggtagagatg ccgtaacgtg tgatagcgtg tgtcagcatc atgacgttgt tgctttcggc
    17401 attcagcgcc agagagatcg gtgccgcgct agcttccgtt gggccaggca cctgtaatac
    17461 ctgcgtcagg ctctcaagat aattttcaac ggcgctacgt tcttcaggtg tgagtttacg
    17521 cgtttgcgca tccgcgaagg cgttggtcca gggcagctga cgccgtctgg atcgcacttt
    17581 tatcagccat cccgcaagta gtgagcaggc cagcaaagca gctaaaaaaa tcacaatggt
    17641 gctcatgctt tccccatctt acttagtctg tcaggcgtgg tcagtcgcac cggacacaat
    17701 acatgattct gcggttggct atcggcaagc gtggagctta acttgagcat cattaagcac
    17761 atcacagtaa ccgctgatca tagcaaaccc tctgaaggcg gaatatcagc aaattcctgg
    17821 agttatttca acctattgac ttttaatttg atttgaggac gattatttat taagttacat
    17881 aaatgtaaat aacatgcaat tcacattgcc gaaagcgtgt ccgatatcgc acaataaccg
    17941 ctgatttcac agcatagatc ccacgatgaa caaatcatta caaaaaccca ccattctgaa
    18001 tgttgaaact gtagcccgtt cccgactgtt taccgtcgag agcgtggatc tggagttcag
    18061 caatggcgtg cggcgtgttt atgaacgaat gcgtccaacc aaccgggaag cagtgatgat
    18121 tgtgccgatt gtggacgatc acctgatcct gatccgcgaa tacgcagtgg gaactgaatc
    18181 ctacgaatta ggtttttcga aaggattaat tgatccgggt gaaagcgtct acgaagccgc
    18241 taaccgcgag ctaaaagaag aggttggatt tggtgcgaac gatctgactt ttttgaagaa
    18301 gctcagcatg gcaccgtctt acttttccag caaaatgaat atcgtggtcg cgcaagatct
    18361 ctacccggaa tcgctggaag gcgatgagcc agagccgcta ccacaggtgc gctggccgct
    18421 ggcgcatatg atggatttgc tggaagaccc tgacttcaac gaagcgcgta atgtcagcgc
    18481 gctgttcctc gtgcgcgaat ggttgaaagg gcaggggcga gtgtaaatgc gtgaagtttg
    18541 cctgatgcac aacgcttatc aggcttatgg ggttcctgca atatattgat ttttcacgat
    18601 tttgttagcc ggataacgcg ttcacccgca tccggcataa acaaatgcac tttgtcagcc
    18661 aactgaaaag gcgccgaagc gcctttttaa tcagaacaat tcctgtgcct caccattatc
    18721 gataatggtc gttcccacct cgtgcaccgc ctgttgtgtc ggctgcgtac cttcgatgaa
    18781 atactcttcg cggctgttgc caccattagc taactgcccg gtgctgcgat cgatattcac
    18841 cgtcacaata cccggaggcg gcgtcagcgg ctgctccggc acaccttcaa gaacggcttt
    18901 catataagcg tcccatgcag gctgggcact cttggcaccg ccttcgtaac ctgagatctg
    18961 atctttaatc gctccggaag ccgttgtatg accgagatta cgacggtgat catcaaagcc
    19021 aatccagacc gaggtcacaa cgcccggacc gtaacccgag aaccacgcat ctttcgaact
    19081 gttagtggta ccggttttcc cgccgatatc gcgacgctgc aaatcacgac ctgcacgcca
    19141 gccggtaccc tgccagcctg gttcaccaaa gatattggtg ttcaaagcac tcttaatcag
    19201 gaatgccagc ggagtgttga tgacgtgcgg tgcgtactcc tgcgcgccag tcttcgccac
    19261 taacgcctga tttgcctgct ccagctgcgg cattggtaca gaaacattct gctgctcgcg
    19321 ggagatagcg acatcttcaa catcgttatt ttccagcacg ttcgatttct gcgtatcacc
    19381 gtaaatcact ggaatatcgc attccgggca ggctactttc ggtttcgctt cgaaaatcac
    19441 gccgccctga tcgttttcaa ttttgctgat aaaccacggg tccactagga agccgccgtt
    19501 cgccatgacc gcgtagccgc gcgccacctg cattggggtg aaggacgctg aacccagcgc
    19561 cagcgattcg gtgtggacaa tgttttgcgc cgggaagccg aagcgttgca gatattctgc
    19621 agcgtagtcg acgcccatcg cccgcattgc acgtaccatc accacgtttt tcgactgacc
    19681 cagcccctga cgtaagcgaa ttggaccagc atactgcggt ggtgagttct tcggctgcca
    19741 gtcagaaccg gcacctgcat cccagcgaga aattggcaca tcgttcaaca tacttgccag
    19801 cgtcagacct ttatccatcg ccgcggtgta gaggaacggt ttgatgttgg aacccacctg
    19861 acgcagtgcc tgggtggcgc ggttaaactt gctctgattg aaatcaaagc caccgaccag
    19921 cgccataacg gcaccatttt gcggattgat cgacaccagc gccgagttca cttccggcac
    19981 ttgtgccagc caccatgcat cgccaacctg acgaacccag atttgctgac ccgtttgcag
    20041 aacatcggtc actttacgcg gcgtcggtcc ctgctgagta tccgaacggt aaggacgcgc
    20101 ccagcgaacg ccttccatac tcaatgcgac ggtcgacccg tccgccagca tcgccgtcgc
    20161 tccctgagga ttggcgctgg tgactgcggc aggcagcagc ggaccatagg tcggcagcgc
    20221 cttcagcgta tcggtaatct tgttgttatc ccacgccgac tcgcccactt tccacagcac
    20281 atttgccggg ccgcgatagc cgtggcgcat gtcgtagtcc agcacgttat tacgtaccgc
    20341 ctgctgcgcg gcctgctgca ctttgcgggt gatggtggtg taaatgcgat aaccgtcttc
    20401 ataggcactt tcgccataac ggttatacat ctcctggcgc accatttcgc tcaggtacgg
    20461 cgcagagaaa gcaatctccg gcgcgtgata attagcgtta atcgcctcag tgcgtgtctg
    20521 atcgaactgt tgctgggtga tatacccttc atccagcatc cgcgacagta cgacgttacg
    20581 ccgcgcgacg gcacgatcca ttgagtagag cggattaaag gtggaaggcg ctttcggcaa
    20641 cccggctatc accgccattt cgttcagcgt cagttggtcg accgttttac cgaaatagac
    20701 ttgtgccgca gcaccgacac cataggcgcg gtaaccaagg taaatcttgt tcagataaag
    20761 ctcgaggatt tcgtctttcg tcagcaactg ttcaatgcga atcgcgagga agacttcctt
    20821 aatcttacgc atcatcgtgc gttctggact gaggaagaaa tttctcgcca gctgctgggt
    20881 aatggtactc gccccttgtg acgcgtggcc ggagaacagc gccacacttg ccgcacggaa
    20941 gatccccacc gggtcaacgc cgtgatgctc gtagaagcgg ctgtcttccg tcgcgataaa
    21001 ggctttcacc atctccggtg ggatttgatc caacgtaacc ggaatacgac gtttctcacc
    21061 gtattgagca atcagctcgc catcggcgct gtaaatctgc atcggaattt gcaggcgaac
    21121 atcttttaat gtcaccacat ccggcagttg tggctcgatg tagcggtata ggccataaat
    21181 cgagcctgct cccagcagaa tgcaacagac tgcaaggatc aaaaaatact ttacgaactt
    21241 cactggaaat ttcccattta gtttcatttg ggcagtttat aaacaaacgc gcggtagtat
    21301 aaaggcaagc cagacgcatt gatatacccg tcagagtgac gggtgataag gagatcatca
    21361 caatgacatt taagatctgg caaattggtt tgcatttaca acagcaagaa gcggcagcgg
    21421 ttgcgatcgt gcggggcgca aaagaatgct ttttgcaacg ctggtggcgg ttgccgctgg
    21481 cgcacgacat tatcaaagat gggcggattg ttgatgcgca gcagctggct aaaacgttgt
    21541 taccgtggag tcgcgaactg ccgcagcgtc atcacattat gttggcgttt cccgccagtc
    21601 gcacattaca gcggtcattt ccgcgcccgt cgatgtccct tggtgagcgg gagcaaacgg
    21661 cctggctgtc agggacgatg gcccgcgagc tggatatgga tccggactcc ctgcgcttcg
    21721 attacagcga agactcactc agccccgctt ataacgtgac tgccgcgcaa agcaaagagc
    21781 tggcaacgct gcttacgctg gcagaaaggt tgcgtgttca tgttagtgcg atcaccccgg
    21841 atgccagtgc attacagcga ttcctgcctt ttttaccttc tcatcagcaa tgtctggcct
    21901 ggcgtgataa cgaacagtgg ctgtgggcga cgcgctatag ctgggggcgc aaactggcgg
    21961 tggggatgac tagcgcgaag gagctggcgg cagcgttatc cgttgatccc gacagcgtcg
    22021 cgatatgtgg cgaaggcgga tttgacccct gggaggccgt ttctgttcgt cagccgccgc
    22081 taccgccgcc tggtggagac tttgccatcg cgctggggct ggcgcttggg aaggcgtact
    22141 gatgaatccg ccaattaatt ttttgccctg gcgacagcaa cgccggaccg cttttctgcg
    22201 tttctggttg ctgatgttcg ttgcgcctct gctgctggcc gtcgggataa cgctaatact
    22261 gcgtctgaca ggcagcgccg aagcgcgcat agacgcagtt ttgcttcagg cggaacaaca
    22321 actcgcccgc agcttacaga taacgaagcc acgtttgctg gagcaacagc agttacgcga
    22381 acagcgttcg caacggcagc gccagcgaca atttacccgc gactggcaat ctgcgctgga
    22441 agcactggcg gcgcttttac ccgagcacgc ctggctgaca acgataagct ggcagcaggg
    22501 aacgctggag atcaaggggc tgacaacaag cattaccgcg ttaaacgcac tagaaacgtc
    22561 actccgccag gatgcgtctt ttcatctcaa tcagcgggga gccacgcagc aggatgcgca
    22621 gggacgctgg caatttgagt atcagttaac aaggaaggtt agcgatgaac atgttctttg
    22681 actggtggtt cgccacatca ccccgcctcc gccagttttg ctgggcattc tggttgctga
    22741 tgttagttac gctcattttt ctatcatcga cacaccatga agagcgcgac gcattaattc
    22801 gactacgggc aagtcatcac cagcagtggg ccgcactgta tcgcctggta gacaccactc
    22861 ccttcagcga ggaaaaaacg ctgccctttt cgccactgga ttttcagtta tccggcgcgc
    22921 aactggtttc ctggcatcca tccgcgcagg gaggtgagtt ggcgttgaaa acgctgtggg
    22981 aagcagtgcc gtcggcattt acacggctgg cagagcgcaa cgtcagcgtg agccgttttt
    23041 cgttaagcgt ggaaggtgat gatcttttgt tcacgctaca actggagatg ccgcatgagg
    23101 gttaaacgct ggttgttggc aggtattgca ttgtgccttt taaccggtat gcgtgaccct
    23161 tttaaatcgc cggaagatct atatgccgga ttagcgaact tagccagtgg cgctatcagg
    23221 ggatggtagg gcgaggcgag cgcatcatcg gtgtaataaa agacgggcaa aagaaatggc
    23281 gacgggtgca gcaaaacgat gtgctggaaa acggctggac aattttacag ctgacgccag
    23341 acgtactaac gctgggtacc gggacaaact gcgaaccgcc acaatggttg tggcaacggc
    23401 aaggagatac aaatgaagca atggatagcc gcactactgt tgatgctgat acccggcgta
    23461 caggcggcaa agccgcaaaa agtgacgctg atggtggatg acgttccggt agctcaggtg
    23521 ttgcaggcgc tggctgaaca ggagaagttg aacctggtcg tgtcgccaga cgtcagcggt
    23581 acggtgtcgt tacatctaac agatgttccc tggaagcagg cactacaaac tgtagtgaaa
    23641 agcgccggac tgataacgcg gcaggaaggc aacattctct cagtgcattc cattgcctgg
    23701 cagaatgaca atatcgcccg ccaggaggcg gagcaggcgc gggcgcaggc aaatctgccg
    23761 ctggaaaatc gcagtataac cctgcaatac gccgacgcgg gagaactggc gaaagcgggg
    23821 gagaagctac tgagtgccaa agggagtatg accgtcgata aacgcaccaa tcgccttttg
    23881 ctacgagata acaaaacggc gttaagcgcg cttgaacagt gggtagcgca aatggatctg
    23941 ccggtcgggc aggttgagct gtcggcgcat attgtcacca ttaatgaaaa aagtttgcgt
    24001 gagttaggcg tgaaatggac gctggccgat gcgcaacacg ctggtggcgt tgggcaagtc
    24061 accacgcttg gtagcgacct ctccgtagcg acggcgacaa cgcatgtcgg ttttaacatt
    24121 gggcgcatca acggacgctt gctggatctt gagctttccg cgctcgaaca aaaacagcag
    24181 ctggatatta tcgccagtcc gcgtctgctg gcctcacatc ttcagcctgc cagcattaaa
    24241 caggagagcg aaattccata tcaggtttcc agcggggaaa gtggcgcgac gtcggtggaa
    24301 tttaaagagg ccgtcctggg gatggaggtc acgcccacgg tgttacaaaa aggtcgcatc
    24361 cggctgaaat tacacatcag ccagaacgtt ccggggcagg tgctacagca ggccgatggc
    24421 gaagtgctgg cgattgataa gcaggagatc gaaacgcagg tcgaggtaaa aagcggagaa
    24481 acgttggcgc tgggcggcat ttttacccgt aaaaataaat cgggtcagga tagcgtaccg
    24541 ttgcttggcg acattccctg gttcgggcaa ttatttcgtc atgacggaaa agaagatgaa
    24601 cgacgcgagt tagtggtgtt tatcacgcca cgactggttt ccagtgagta aacagccgta
    24661 aaagcggtaa tgtttttatg ctgaacgtgt ttcatctatt tgacgcgcgc aggtatttag
    24721 catacaagga gtaccgattt gagagttggt gctcttcgct gcctgcgttc catgatgatg
    24781 atttatcatt caggcggcat tttgctgtct tttttacgct aatcttaccc ggtgatttat
    24841 cgccagagcg gtggtagcaa ggcagcgcgc ttgcagcgac cagatatgca gagggatggg
    24901 tgatttattc agttgccaaa cccgctggag tattgagata attttcagtc tgactctcgc
    24961 aatatcttat gaggtttcag ttcatgtcct gcggcgctct ctgagcgaag cgggtttatc
    25021 attaacgaat agtcttagta gtaccgaaaa aatggcagag aaacgcaata tctttctggt
    25081 tgggcctatg ggtgccggaa aaagcactat tgggcgccag ttagctcaac aactcaatat
    25141 ggaattttac gattccgatc aagagattga gaaacgaacc ggagctgatg tgggctgggt
    25201 tttcgattta gaaggcgaag aaggcttccg cgatcgcgaa gaaaaggtca tcaatgagtt
    25261 gaccgagaaa cagggtattg tgctggctac tggcggcggc tctgtgaaat cccgtgaaac
    25321 gcgtaaccgt ctttccgctc gtggcgttgt cgtttatctt gaaacgacca tcgaaaagca
    25381 acttgcacgc acgcagcgtg ataaaaaacg cccgttgctg cacgttgaaa caccgccgcg
    25441 tgaagttctg gaagcgttgg ccaatgaacg caatccgctg tatgaagaga ttgccgacgt
    25501 gaccattcgt actgatgatc aaagcgctaa agtggttgca aaccagatta ttcacatgct
    25561 ggaaagcaac taattctggc tttatataca ctcgtctgcg ggtacagtaa ttaaggtgga
    25621 tgtcgcgtta tggagaggat tgtcgttact ctcggggaac gtagttaccc aattaccatc
    25681 gcatctggtt tgtttaatga accagcttca ttcttaccgc tgaaatcggg cgagcaggtc
    25741 atgttggtca ccagcgaaac cctggctcct ctgtatctcg ataaggtccg cggcgtactt
    25801 gaacaggcgg gtgttaacgt cgatagcgtt atcctccctg acggcgagca gtataaaagc
    25861 ctagctgtac tcgataccgt ctttacggcg ttgttacaaa agccgcatgg tcgcgatact
    25921 acgctggtgg cgcttggcgg cggcgtagtg ggcgatctga ccggcttcgc ggcggcgagt
    25981 tatcagcgcg gtgttcgttt cattcaagtc ccgacgacgt tactgtcgca ggtcgattcc
    26041 tccgttggcg gcaaaactgc ggtcaaccat cccctcggta aaaacatgat tggcgcgttc
    26101 taccagcctg cttcagtggt ggtggatctc gactgtctga aaacgcttcc cccgcgtgag
    26161 ttagcgtcgg ggctggcaga agtcatcaaa tacggcatta ttcttgacgg tgcgtttttt
    26221 aactggctgg aagagaatct ggatgcgttg ttgcgtctgg acggtccggc aatggcgtac
    26281 tgtattcgcc gttgttgtga actgaaggca gaagttgtcg ccgccgacga gcgcgaaacc
    26341 gggttacgtg ctttactgaa tctgggacac acctttggtc atgccattga agctgaaatg
    26401 gggtatggca attggttaca tggtgaagcg gtcgctgcgg gtatggtgat ggcggcgcgg
    26461 acgtcggaac gtctcgggca gtttagttct gccgaaacgc agcgtattat aaccctgctc
    26521 acgcgggctg ggttaccggt caatgggccg cgcgaaatgt ccgcgcaggc gtatttaccg
    26581 catatgctgc gtgacaagaa agtccttgcg ggagagatgc gcttaattct tccgttggca
    26641 attggtaaga gtgaagttcg cagcggcgtt tcgcacgagc ttgttcttaa cgccattgcc
    26701 gattgtcaat cagcgtaaca acaagaaagg tcaggccgct tatcaagcgg tctattagct
    26761 tcaggttaat tgcaacgtgg taagcattaa ccttttagtg gggtgttaaa tggatgaatt
    26821 caaaccagaa gacgagctga aacccgatcc cagcgatcgt cgtactggtc gttctcgtca
    26881 atcttctgaa cgttctgagc gtactgaacg tggcgaaccg cagatcaatt ttgatgatat
    26941 tgaacttgat gacactgacg atcgccgtcc gactcgtgcg caaaaagagc gcaatgagga
    27001 accggaaatc gaagaagaaa ttgacgaatc cgaagatgaa accgtggatg aagagcgcgt
    27061 agagcgtcgt ccgcgtaagc gcaaaaaagc agccagtaaa cccgcttctc gtcagtatat
    27121 gatgatgggc gtcggcattc tggttctact gctgttgatc atcggtatcg gttctgcgct
    27181 aaaagccccc tcgaccactt ccagcgatca aaccgcgtct ggcgagaaga gtattgatct
    27241 tgctggcaat gcgaccgatc aggcgaatgg cgtgcagcca gcgccgggaa ccacgtctgc
    27301 ggaaaatact cagcaggatg tttctctgcc accgatctct tctacgccga ctcaagggca
    27361 aaccccggtg gcaacggatg gtcaacaacg tgttgaagtg cagggtgacc tgaacaatgc
    27421 gctgacccag ccacaaaatc agcaacagct gaacaatgtg gcggtcaatt ccacgttgcc
    27481 gactgaaccc gcaacggttg cgcctgttcg caatggcaat gcatcgcgtg acacggcgaa
    27541 aacgcaaacc gctgaacgtc cgtccactac gcgcccagtt cgtcagcagg cggtgattga
    27601 accgaaaaaa ccgcaagcaa ccgtgaaaac ggagccgaag ccggtagcac agacgccgaa
    27661 gcgtactgaa cctgaaccag ctgcccctgt ggcgagcacg aaggcaccgg ctgcgacttc
    27721 tacgccagca ccaaaagaga cggcgactac ggctccagta cagacggcat ccccggcgca
    27781 aaccacggca acaccagccg ctggagggaa gaccgcaggt aatgttggtt cgttgaaatc
    27841 ggcaccgtcc agccattaca ctctgcagct gagcagttcc tctaactacg acaacctgaa
    27901 cggttgggcg aagaaagaga atctgaaaaa ctacgttgtc tatgaaacga cgcgtaatgg
    27961 tcagccgtgg tatgtcctgg tttctggcgt gtacgcttcg aaagaagagg cgaaaaaagc
    28021 ggtatctaca ttgccagcag atgttcaggc caaaaacccg tgggcgaaac cgctgcgtca
    28081 ggtacaggcc gatctgaagt aatcaaggtt atctcccgca atggtttatc gttgcgggag
    28141 ctgcctgaag cgctggatgc tgtcggagct ttctccacag ccggagaagg tgtaattagt
    28201 taatcggcat gaagaaaaat cgcgcttttt tgaagtgggc agggggcaag tatcccctgc
    28261 ttgatgatat taaacggcat ttgcccaagg gcgaatgtct ggttgagcct tttgtaggtg
    28321 ccgggtcggt gtttctcaac accgactttt ctcgttatat ccttgccgat atcaatagcg
    28381 acctgatcag tctctataac attgtgaaga tgcgtactga tgagtacgta caggccgcac
    28441 gcgagctgtt tgttcccgaa acaaattgcg ccgaggttta ctatcagttc cgcgaagagt
    28501 tcaacaaaag ccaggatccg ttccgtcggg cggtactgtt tttatatttg aaccgctacg
    28561 gttacaacgg cctgtgtcgt tacaatctgc gcggtgagtt taacgtgccg tttggccgct
    28621 acaaaaaacc ctatttcccg gaagcagagt tgtatcactt cgctgaaaaa gcgcagaatg
    28681 cctttttcta ttgtgagtct tacgccgata gcatggagcg cgcagatgat gcatccgtcg
    28741 tctattgcga tccgccttat gcaccgctgt ctgcgaccgc caactttacg gcgtatcaca
    28801 caaacagttt tacgcttgaa caacaagcgc atctggcgga gatcgccgaa ggtctggttg
    28861 agcgccatat tccagtgctg atctccaatc acgatacgat gttaacgcgt gagtggtatc
    28921 agcgcgcaaa attgcatgtc gtcaaagttc gacgcagtat aagcagcaac ggcggcacac
    28981 gtaaaaaggt ggacgaactg ctggctttgt acaaaccagg agtcgtttca cccgcgaaaa
    29041 aataattctc aaggagaagc ggatgaaaca gtatttgatt gccccctcaa ttctgtcggc
    29101 tgattttgcc cgcctgggtg aagataccgc aaaagccctg gcagctggcg ctgatgtcgt
    29161 gcattttgac gtcatggata accactatgt tcccaatctg acgattgggc caatggtgct
    29221 gaaatccttg cgtaactacg gcattaccgc ccctatcgac gtacatctga tggtgaaacc
    29281 cgtcgatcgc attgtgcctg atttcgctgc cgctggtgcc agcatcatta cctttcatcc
    29341 agaagcctcc gagcatgttg accgcacgct gcaactgatt aaagaaaatg gctgtaaagc
    29401 gggtctggta tttaacccgg cgacacctct gagctatctg gattacgtga tggataagct
    29461 ggatgtgatc ctgctgatgt ccgtcaaccc tggtttcggc ggtcagtctt tcattcctca
    29521 aacactggat aaactgcgcg aagtacgtcg ccgtatcgac gagtctggct ttgacattcg
    29581 tctggaagtg gacggtggcg tgaaggtgaa caacattggc gaaatcgctg cggcgggcgc
    29641 ggatatgttc gtcgccggtt cggcaatctt cgaccagcca gactataaaa aagtcattga
    29701 tgaaatgcgc agtgaactgg caaaggtaag tcatgaataa gtttgaagat attcgcggcg
    29761 tcgcttttga tcttgatggt acgctggtcg acagtgctcc tggtcttgct gctgcggtag
    29821 atatggcgct gtatgcgctg gagttgcccg tcgcaggtga agaacgcgtt attacctgga
    29881 ttggtaacgg cgcagatgtt ctgatggagc gcgcattgac ctgggcgcgt caggaacgtg
    29941 cgactctgcg taaaacaatg gggaaaccgc ccgttgatga cgacattccg gcagaagaac
    30001 aggtacgtat tctgcgtaaa ctgttcgatc gctactatgg cgaggttgcc gaagagggga
    30061 cgtttttgtt cccgcacgtt gccgatacgt tgggcgcgtt gcaggctaaa ggcctgccgc
    30121 taggcttggt caccaacaaa ccgacgccgt tcgtcgcgcc gctgctcgaa gccttagata
    30181 ttgccaaata ctttagcgtg gttatcggcg gcgatgatgt gcaaaacaaa aaaccgcatc
    30241 cggacccgct gttactggtg gctgagcgga tgggaattgc cccacaacag atgctgtttg
    30301 tcggcgactc acggaatgat attcaggcgg caaaagcggc aggttgccca tcagttggct
    30361 taacctacgg atacaactac ggcgaggcta tcgatctcag ccagcctgat gtaatttatc
    30421 agtctataaa tgaccttctg cccgcattag ggcttccgca tagcgaaaat caggaatcga
    30481 aaaatgacta agcccatcgt ttttagtggc gcacagccct caggtgaatt gaccattggt
    30541 aactacatgg gtgcgctgcg tcagtgggta aacatgcagg atgactacca ttgcatttac
    30601 tgtatcgttg accaacacgc gatcaccgtg cgccaggatg cacagaagct gcgtaaagcg
    30661 acgttggata cgctggcctt gtatctggct tgtggtatcg atcctgagaa aagcaccatt
    30721 tttgttcagt cccacgtacc agaacatgcg cagttaggct gggcactgaa ctgctatacc
    30781 tacttcggcg aactgagtcg catgacgcag tttaaagata aatctgcgcg ttatgccgag
    30841 aacatcaacg ctggtctgtt tggctatccg gtgctgatgg ctgcggacat cctgctgtat
    30901 caaactaatc tggtaccggt gggtgaagac cagaaacagc acctggaact gagtcgcgat
    30961 atcgcccagc gtttcaacgc gctgtatggc gagatcttta aggtgccgga gccgtttatt
    31021 ccgaaatctg gcgcgcgcgt aatgtcgctg ctggagccga ccaagaagat gtccaagtct
    31081 gacgataacc gcaataacgt tatcggcctg ctggaagatc cgaaatcggt agtgaagaaa
    31141 atcaaacgcg cggtcactga ctccgacgag ccgccggtag ttcgctacga tgtgcagaac
    31201 aaagcgggcg tttccaacct gctggatatc ctttctgcgg taacgggcca gagcatccca
    31261 gaactggaaa aacagttcga aggcaagatg tatggtcatc tgaaaggtga agtggctgat
    31321 gccgtttccg gtatgctgac tgaattgcag gaacgctatc accgtttccg caacgatgaa
    31381 gccttcctgc aacaggtgat gaaagacggc gcagaaaaag ccagtgtaca cgcttcccgt
    31441 acgctgaaag cggtgtacga agcgattggt tttgtggcga agccgtaata tcaaaacgct
    31501 cggacggtcc cctcgcccct ttggggagag ggttagggtg aggggagcag gcaagcgcca
    31561 gtgggaatta ccctttgctc gcattgtcga ataaatcact tcgtcatcac tctgaaaccg
    31621 gggaaacccg gtttttttat cctcttttcg caacaacccg aaaaaatgtg aagcgcctcg
    31681 ccgtttccac atctcgtcgt cgccacttat cttcatttta atgaagatat aaatattcat
    31741 tttattgaaa atatattatg cgtcgaacgt ttatcaaaaa agaaggcgtc gtcatcacga
    31801 cactggcccg ttatttgttg ggtgaaaaat gcggtaatcg actgaaaacc atagatgagc
    31861 tggcaactga atgccgttca tcggttggcc tgacgcaggc cgcgttgaaa acgctggaat
    31921 caagcggagc gatacggatt gaacgccgtg ggcgcaatgg cagttatctt gtcgagatgg
    31981 ataacaaagc attgctgagt catgtggata tcaacaacgt ggtatgtgcg atgcctttgc
    32041 cctatacccg cttgtacgaa ggtttagcga gcggattgaa agcccagttt gatggcattc
    32101 ctttttacta tgcgcatatg cgcggcgcgg atattcgcgt ggagtgttta cttaacggtg
    32161 tctatgacat ggcagtagtt tcccgcctgg cggcggaaag ttatctcagc caaaacaatt
    32221 tgtgcatcgc cctcgagctt ggcccgcata cgtatgttgg tgaacatcaa ctgatatgcc
    32281 gcaaaggtga gtctggaaat gttaagcgag taggtctcga tagtcgttcg gcggatcaga
    32341 aaatcatgac cgatgtcttt tttggcgata gtgatgtgga acgagtcgat ctctcttatc
    32401 acgagagttt acaacgcatt gttaaaggcg atgtcgatgc ggtgatctgg aacgtggtgg
    32461 cggaaaacga actgaccatg ctgggattag aggcgacgcc gctaacagac gatccgcgat
    32521 ttttacaggc caccgaagcc gtggtcctga cgcgagtcga tgattaccca atgcaacaat
    32581 tgctgcgtgc tgttgtagat aaacacgccc tgcttgccca tcaacaacgg gtggtgagtg
    32641 gggaacagga acccagttat taaacgaaag gcatctgata tggaaaccag actcaacctg
    32701 ctttgcgagg caggcgttat tgataaggac gtctgcaaag gcatgatgca ggtcgtcaac
    32761 gtactggaaa aagagtgcca tctgccggtg cgcagtgagc aaggaacgat ggcgatgaca
    32821 catatggcga gtgcactgat gcgcagtcgc cgtggtgaag aaatagagcc gctggataac
    32881 gagttgctgg cggaactggc gcaatccagc cactggcaag ccgttgtgca attgcatcag
    32941 gagttgttga aggaattcgc tctggaagtt aacccgtgtg aagaaggcta ttttctggcc
    33001 aatctttatg gattatggat ggctgctaac gaagaggttt gaagctcccg acaaatccac
    33061 gtcgcacaac ctaaatattc aaaaaaatga atatttaatt caaggaacag aaacgaggca
    33121 acaagatgtt tgtagaagca ttgaaacgcc agaacccggc gctgatttcc gccgcactaa
    33181 gtttgtggca gcagggcaaa atcgccccgg acagctgggt gatcgacgtg gatcagatac
    33241 tggaaaacgg taagcggctc attgagacgg cgcggcttta cggcattgaa ctgtatctga
    33301 tgaccaagca atttggtcgt aatccgtggc tggcggaaaa attgctggca ttaggctaca
    33361 gcggcattgt ggcggtggat tacaaagagg cgcgagtcat gcgccgcgct ggtttgccag
    33421 tggcgcatca ggggcatctg gtacaaatcc cttgtcatca ggttgctgac gccgttgaac
    33481 agggcaccga catcatcacc gtgtttactc tcgacaaagc gcgggaagtt tctgcggcag
    33541 cggttaaggc cggacgggtc cagcctgtgc tgctcaaagt ttatagcgac gatgattttc
    33601 tttatccggg gcaggagagc ggttttgtac agcattcgct gcacgaggtg attgctgaaa
    33661 ttaagaaact gccggggctg catttagccg gactaaccca tttcccttgc ctgctttggg
    33721 atgaggctgc tgggaaagtt ttgccaacac cgaatcttca cacgctggta caagcacggg
    33781 atcaactggc gaaatctggt attgcgattg agcaactgaa cgtaccttca gcgaccagct
    33841 gcacttcgct gccattgctg gcggaatacg gtgcgactca tgccgaaccc ggtcatgcgc
    33901 tgaccggtac tattccggca aaccagcagg gcgatcagcc tgaacgtatc gcgatgctct
    33961 ggttaagtga aatctcccat catttccgtg gcgacagcta ctgctacggc ggcggttact
    34021 accgtcgtgg tcatgcgcaa catgcgctgg tgtttacgcc agaaaatcaa aagattactg
    34081 aaaccaatct caaaactgtg gatgacagca gtatcgacta caccctgccg ctggcaggcg
    34141 agtatccggt gagcagtgca gtggtgctct gttttcgcac gcagattttt gtcacccgta
    34201 gcgatgtggt gctggtgtcc ggtattcagc gcggcgaacc agaaatagtt ggtcgttatg
    34261 acagcctcgg caaccctctg gaggcataaa tggcgcgatt tgtggtgtta gtgattgata
    34321 gctttggcgt aggggcaatg aaagatgtca cgctggtgcg cccgcaagat gccggggcga
    34381 atacctgtgg tcacatcctg agccagttgc cgcatttgca gctaccaacg ctggagaagc
    34441 tggggctaat caacacattg ggttatgcgc caggcgatat gcagccgtca gattccgcaa
    34501 cctggggcgt ggcagagctg caacatgaag gtggcgatac ctttatgggg catcaggaaa
    34561 ttttaggcac gcgcccgtta ccgccgctgc ggatgccttt tcgcgatgtg attgaccgtg
    34621 ttgagcaggc attagtttcc gctggctggc aggtggagcg ccgtggcgat gatctgcaat
    34681 ttctgtcggt caatcaggcg gttgcgattg gcgataatct cgaggcggat ttaggccaag
    34741 tctataacat taccgccaat ctctctgtga tctcttttga cgacgcaatc aaaattggtc
    34801 gtatcgtgcg tgaccaggta caggtcggtc gggtcattac atttggcggc ctgttaacca
    34861 acagtcagcg tattctcgat gccgcagaaa gcaaagaagg gcgctttatt ggtattaatg
    34921 cgccgcgttc tggcgcttat gacaacggct tccaggtcgt gcatatgggc tacggtgttg
    34981 atgaaaaagt gcaggtgccg caaaaactgt atgaagcggg cgtgccaacc gtgctggtgg
    35041 gtaaggtggc agatatcgtc agcaatcctt atggtgtgag ctggcaaaac ctggtggata
    35101 gccagcggat tatggatatc accctcgacg aatttaacac ccatccgacg gcgtttattt
    35161 gcatcaatat ccaggaaacc gacctcgctg gtcatgcaga agacgtcgca cgttatgccg
    35221 aacgtttgca ggtcgttgac cgtaaccttg cccggcttgt tgaggcgatg cagccagatg
    35281 attgcctggt cgtgatggcg gatcacggta acgatccgac cattggtcac agccaccata
    35341 cccgtgaagt ggtgccagtg ctggtttatc agcaagggct ggtcgccacg cagctcggtg
    35401 tgcgcaccac gctttctgat gtgggagcta ccgtgtgtga atttttccgt gcgccaccgc
    35461 cacaaaatgg tcgctctttt ctttcctccc tccggtttgc aggagacacc ctatgagttt
    35521 tgatccgacg ggttacaccc tggcccatga gcatctgcat attgatctct ccggctttaa
    35581 aaacaacgtg gactgccgcc ttgatcagta tgcgttcatt tgccaggaga tgaacgacct
    35641 gatgacccgg ggtgtacgta atgtgattga gatgaccaac cgttacatgg ggcgcaatgc
    35701 gcaatttatg ctcgatgtaa tgcgcgaaac cgggatcaac gtggtggcct gtactggtta
    35761 ttaccaggat gcgtttttcc cggaacatgt ggtgacccgc agcgtgcagg aactggcgca
    35821 ggagatggtc gatgaaattg aacagggtat cgatggcacg gagctgaaag ccgggatcat
    35881 cgcggagatc ggctccagcg aaggaaagat tacgccgctg gaagagaagg tatttattgc
    35941 cgctgcgctg gcgcataacc agaccggacg cccgatctcc acgcatacgt cgttcagcac
    36001 catggggctg gaacaactgg ctctgttgca agcccacgga gttgatcttt cgcgcgtcac
    36061 cgttggtcac tgcgatctga aagacaacct cgacaacatt ttgaagatga tcgatctcgg
    36121 cgcgtacgtg cagttcgaca ccattggtaa gaacagttac tacccggacg aaaagcgcat
    36181 tgcgatgctc cacgcgctac gtgaccgtgg gttgctgaac cgcgtcatgc tgtcgatgga
    36241 tattacgcgc cgctcccatt taaaagccaa tggtggttat ggctatgact atttattgac
    36301 cacctttatt ccgcaattgc gccagtcagg attcagtcag gccgatgtgg atgtgatgtt
    36361 acgtgaaaat ccctctcaat ttttccaata aggacagact catgaaaaag attggcgttg
    36421 caggcttaca gcgtgagcag attaaaaaaa ccattgaagc gacggctcct ggctgttttg
    36481 aagttttcat tcacaacgac atggaagcgg caatgaaggt gaaatccggg caactagatt
    36541 attacattgg cgcgtgtaat accggtgcgg gcgcggcatt gtcgattgcc atcgcggtga
    36601 ttggctataa caaaagttgc accattgcca aaccaggcat taaagcgaaa gacgagcata
    36661 tcgccaaaat gatcgctgaa gggaaagtgg cgtttggcct ttccgttgag cacgtcgaac
    36721 acgcgattcc gatgttgatt aaccatctga aataaaaggc acgactatgg atctgtatat
    36781 tcagattatc gtggtggcgt gcctgacggg tatgacatcg cttctggagc atcgctcggc
    36841 ggctgttttt catgacggca tccacccgat cctgccgcaa ctgattgaag gctatatgaa
    36901 ccgtcgcgag gcggggagta tcgcttttgg tctgagcatt ggttttgtgg cctcggtggg
    36961 gatctctttt accctgaaaa ccgggctgct caacgcatgg ttactctttc ttcctaccga
    37021 tatcctcggc gtactggcga taaacagcct gatggcgttt ggtcttggcg ctatctgggg
    37081 cgtgttgatc cttacttgcc tgttgccagt taaccagctg ctgaccgcgc tgccggtgga
    37141 tgtattaggt agcctcgggg aattaagctc gccggtggtt tctgcttttg cactgttccc
    37201 gctggtggcg attttctacc agtttggctg gaagcaaagt ctggtcgccg ccgtggttgt
    37261 tctgatgacc cgtgtggtag tcgtgcgcta tttcccacat cttaaccctg aatccatcga
    37321 aatctttatt ggcatggtga tgctgctggg gatcgcgata actcacgacc tgcgtcatcg
    37381 tgatgaaaat gacatcgatg ccagcgggct ttcggtgttt gaagagcgca cgtcgcggat
    37441 tatcaaaaac ttaccttata tcgccatcgt gggagcattg attgccgccg ttgccagtat
    37501 gaagattttt gctggtagcg aagtttcgat cttcacactg gagaaagcgt attccgcagg
    37561 cgtgacgccg gaacaatcgc aaacgctgat taatcaggcg gctctggcag aatttatgcg
    37621 cggactgggg tttgtgccgt tgattgccac caccgcctta gcaacgggtg tgtatgcagt
    37681 tgcgggcttt acctttgttt atgcggtggg ctatctctcg ccgaatccga tggttgcagc
    37741 ggtattaggc gcagtggtta tttcggcgga agtcttgctg cttcgttcga tcggcaaatg
    37801 gctgggacgc tacccgtcgg tgcgtaatgc gtcggataac atccgtaacg ccatgaatat
    37861 gctgatggaa gtggcgctgc tggtcggttc gattttcgca gcaattaaga tggcgggtta
    37921 taccggattc tctatcgcgg ttgccattta cttcctcaac gaatccctgg gccgtccggt
    37981 acagaaaatg gcggcaccgg tcgtggcagt aatgatcacc ggtattctgc tgaatgttct
    38041 ttactggctt ggcctgttcg ttccggctta aggaaggcac ctatgaagac gtttcctttg
    38101 caaagcctga ctcttgctga agcgcagcaa aagcagtttg cgctggtgga cactatttgc
    38161 cgccattttc caggcagcga gtttcttgct ggcggtgatt tagggttaac tccaagtctg
    38221 aatcaaccgc gtattaccca acgggtggag caggtgctgg ctgatgcatt tcacgcacag
    38281 gccgcggcgc tggtgcaggg cgcggggact ggcgcgattc gcgccgggct ggcggctttg
    38341 ctcaaaccgg ggcagcgtct tctggtgcat gacgcgcctg tttacccgac gacacgggtt
    38401 attattgagc agatggggct gacgcttatt actgctgatt tcaatgacct gtcggcactg
    38461 aagcaggtcg tcgacgagca acaacctgat gccgcgctgg tgcagcatac gcgccagcag
    38521 ccgcaggaca gctatgtgct ggcagatgtg ctggcaacgt tgcgcgcggc aggtgttcca
    38581 gcgttaaccg atgataacta tgcggtgatg aaggtagcgc gaatcggctg tgaatgcggc
    38641 gcgaatgtct cgacattctc ctgcttcaag ctgtttgggc cggagggcgt tggtgcagtg
    38701 gttggcgatg ccgatgttat tagtcggatt cgcgccacgc tttactccgg cggtagccag
    38761 atccagggct cacaggcgct ggaagtcttg cgtggtctgg tgttcgcgcc agtgatgcat
    38821 gcggtgcagg cgggagtatc cgaacggttg ctggctttgc ttaacggcgg cgcggtggcg
    38881 gaagtgaaaa gcgccgtcat tgccaatgcg cagtcgaagg tgttgattgt cgagtttcat
    38941 cagctgattg ccgccagggt gctggaagaa gcgcaaaagc gtggcgcgtt gccttacccg
    39001 gtcggtgcag agtcgaaata tgaaatcccg ccgctcttct atcgactttc aggaacgttt
    39061 cgccaggcga atccacaact cgaacattgt gcgattcgca ttaacccgaa tcgcagcggt
    39121 gaagagacgg tgctgcggat tttgcgtgag agtattgcga gtatttaatg ctggatagca
    39181 ccgttcggca atcaagctaa attccgcact gaacggtccc ctcgcccctt tggggagagg
    39241 gttagggtga ggggaacgtg cgggatgtaa aattcagtgc gccagcatcg ccggatgcga
    39301 tgctggcgca tcttatccgg cctacggtta atgtctttta ttatcaatcg ttaaggtgat
    39361 gcgattggcg cggcagaaga gttcggagac gtgcaccggt ttgtcattct gatcaaaggc
    39421 gattttgctg atgcgaaaca acggttcgcc cagttcgcat ttcagccatt ttgcctgcgg
    39481 gcgggtggcg gcaaagatat cgatggtctt tttgtcgctg accactcgcg tgtcaaaacg
    39541 ctcctgaaat aactgatagg tggagcttcc ttcgacgtaa atctcatcaa agtcaggata
    39601 acgcgacagc gggatccatg aactatcaat aaacagcggc tctttatcga gatacatcac
    39661 ccggcagaga tggaacactt cgctgtttcc ggggatgttc agcttttcgc aaaacggcgc
    39721 ggcgctgacc cgttcctgtt cgatcacttt ctctttcgtc gacttgcctt gtgagacgcc
    39781 aaaatcggta aaaccactga cagtaagcag ggcgttttca actttctggc tttgtacaaa
    39841 ggtgcctttt ccctgccagc ggatcagtac gccgtctgcc actaagtcgc tgatggcttt
    39901 gcgaatggta atgcggctga cgttatattg tgtacaaagc tcgttttcgg tagggatctg
    39961 ttgcccggcc tggtaaaccc cctgcgcgat atcatccagc agccgctggc ggacggtagc
    40021 gtagaggagt tgatgagagt agcggtccgt agctgacata atcgaccctg aaataaagaa
    40081 tgaattactc ggattatagg gtcgttttcc ggcaagtaac agcacgttgg cgtgaagatg
    40141 ctcacgccaa cgttatacct accaggcacc gtggtactga atggttttcg ccgcgcacgc
    40201 cgttccctgc gccatcgcct gcggtaatgt catccccgca gaccagccgc aaaggaatcc
    40261 ggcaatgaac gaatctccgg cacccatggt gtcgataacc gtcaccggtt caggagcctg
    40321 acgccagaac tgcgcgccat cccaggcaat gctgccgttt tcacccagcg tgacaatcac
    40381 tgttcctgcg ccacgggcaa caatcgcttt catcttcaga cgcagcgctt cgtcttcttg
    40441 cggtgcggag gcaaaggcaa aatcgagatg cggcaccagt gtctgccaga gcgggctgtc
    40501 ccacttgtcg gagaagtcga aagcggtaag tttgcccgca gcgtgcagct gtgggaatgc
    40561 gtcttccgca tgtccccaga ttgccgcgtg cacaatgtca tactgcgcca gccaggcgta
    40621 atcctcttca ctcagggcaa agtcggccat cacgccttcg gtgtagtcgc caaaaacgcg
    40681 atcattgtcg tgcagttcca cctgagtttg tgcggtaacg ccgtgtttcg tatggacatg
    40741 gctgatatcg acgcccatgc gggcgagatc ctgcttcagc tttgtgccgt agtcatcgtc
    40801 acccacccag gtaatgcatc ccggctgtat gccgtagcga gtgcagtaca ccgccacatt
    40861 gaccgcatta ccgccagaaa acgctttatt cagttgcggg tagatatcga cgcagttatc
    40921 gccgattgtc gccagggttt tcatctctca tcctccggca gcagcgcgcg aaagcgttcc
    40981 agcgcctggc gggcatagag tctgggctcg ttcatataca tcgtcaccag ctccaccgta
    41041 gagtaaccct catagccccg atcaataata tcgcgcatca gttcccgcag cggcattttg
    41101 ccttctccag gaatgtaatg cgtgtcgctg gccccgtcgc tgtcgacaat atgcagatga
    41161 cgtaatttat cgcccagttt gtcgaaataa ctcatcaccg gttccgcctg gacatacggc
    41221 gcgcaaatgt cgaccatgct gaacaggcgc ggcgaaggca ccagcgccag cgcatgaagc
    41281 acatcattag cattacacac gacgttcgat tcatacggcg ttaacggttc gaggatcaga
    41341 tccatgccga tgttttccgc gtactcacac agttcgctta agttctctgc cagccgcccc
    41401 cagataacgt taggcggcgt gagatagccc gcgtgggccg cagaaatcag cgtataaccc
    41461 gcgttcatct cttttgccat atctatcgcc agcttgatca tgtccaggct ttcacgacgc
    41521 atatgttcat cgcccagcat catgttatac ggatagccgt tggtttctgg cgtgtaaccg
    41581 ataatcggca tctgatacgt ctgcgccagc gccttgattt gtttgatgcc gcccgctttt
    41641 aagtccggcg cgaacgcgtg cgggcgaccg ccccaaattt cgatgccgtc gtaacccagc
    41701 tcgcttgcat cacgaaatgc gtgttcaata ggcagccgct ggtggccgca ggtaaacata
    41761 cctgttttca tcgggtaact ccttgcgtaa atccggcccg ttgccgggcc gggagaggga
    41821 ttaatattcc accagaccac cgtaatagcg gcgttcatcc gggttgtgat ctttgtaaat
    41881 agacaggtag tagcagagcc actccattgg cacgaacatc aggaacggtg ccagccacgg
    41941 gtgcagccct tgcgaaattt cggcgtaatc gatgacgatc acgttgtcag tacgctgttt
    42001 aacaaagtta atggcgcgtt cggtggtgtg gcgactttca tcattgccga gcaggaacag
    42061 gaacggaacg cccggttcga caatctccag cgggccatgg cggaactctc cgctctcaat
    42121 cacgcagccg tgcgtccagg taaattccat cagcgttaca atgccttctt tgtaacccag
    42181 cggacgcagc ggacccgcag caacggtata aatcatcggc cactggctgg ccagttcacc
    42241 aagctggcgg cctttttctt cccatgtgcg taccagatga ccgagcgcat tcggcaactg
    42301 cttgagatcg tttttgatct tgccgatttc cgcgttcggc gcgaggcggg tgatcatctc
    42361 cagcaccacg ctgtagcaga gcagcaggtg aatttcccag atacagtcgg cctgataatc
    42421 aatgctaaat tccgccgccg aggtaatcgg gctgtccgcg cgtttggtga acgccgcagt
    42481 gagtgctccg caggcccggc ccagctccag cgctttgatt acctcttccg ttttaccgta
    42541 gtcagaaacg ccaatcaccg cgcaacgatc gtcgaggcga tacggggtgt tatcgcagaa
    42601 ctcccagccg gaaatggcgt agacctgaag atcggaaaag cgatccgcca gatgtttcgc
    42661 cgtttgcgcc gcgttgagtg gcgagccgca ggcaacgaaa taaatacgat caatgtcgcg
    42721 cttcaccatc tcttccacga tggcgtgcac cagcggaacg tcatggctga gaactttttc
    42781 cacttcctga accatatttt cggtgaccag aaagtccacg gtgcttttat caatatccaa
    42841 cattttattt tcttcagata acattacgac aaagcgttga gctggcggct acgcttcgcc
    42901 cagaaggcgt aagcaggcag accagtagca ataacgatgg cggcgcagat aaggccggga
    42961 atcggtgccc agacaaaggt tgaggcgacc agaatgaggc ttgacgcaat ggcgagggtg
    43021 gtcatcagcc cgaaagccgg agtacgccac agcggtttgt aatcgtcgcg tttacgacac
    43081 cagatgatgg agccgaaggt gagggtattt ttgaaacaca tcaccagggt gaaataaccc
    43141 agcaggctgg tgagatcgga aacgaagatg aagaagatcc ccagcgcccc ttgcaggatg
    43201 atggagacat ccggcgtgtt gtatttcgga tgcgcatggc cgaagcattt aaagaacagg
    43261 ttgtctttcg ccatcgcgta ttccagccgc ggctggtaca tcacgcagct ggaaagcgaa
    43321 ccaagaatga cgatcatcgc cgtgatggca acaaagagcc cagcggtgct gccgagtgcg
    43381 gggatctagg tcagggcgtc ggaaatcggc gtttcagaat tggcgagttt gtcgaagggc
    43441 atcaggccgg aaatcaccag cgccagcagg gtgtagagca ccagaaccag cagacaggaa
    43501 ccaatcagcg ctcgtggcat ggtttttccg gggtttttaa tttcgccggt catataacag
    43561 atagaggcca tgccggtata cgaccaactg gtggcagaga tccccgccag cagcgccata
    43621 aagctgcccg ttgcgccaat cgcagtggtg gtaagggcgg caaaattctc cgctttaaac
    43681 cagaagatcc caaggccaat gacgatagtg aacgggataa ttttggcgat ggtaattagc
    43741 gtctgaaacg ctgcgccgcc ttcaacggag cgcaggtgta gcaacataaa ggcgataatt
    43801 aatccggcgg cgataaattt accgagcaac ggatcgatag gcgttaaaaa gccaagattg
    43861 ctgacaatcg ccagcgccat aatcgacaat gacggcgcat cgttggccca gaagctggcc
    43921 cagccggaga ggaaagccag cggtcggctt ccggcatttt tcagataaac ataatctgcg
    43981 ccattttccg gataagcggt ggatagttcc gcatagacgc acatttgcgg gatcacaatt
    44041 aacccgccaa tgacaaacgc gagcaccgta agccacggcg tgcccgctgc ttttgccact
    44101 tcacccacag atacaaaaat accggagccg acggttgtcc cgacggcgat tgcaagaacg
    44161 gcccaaaatc cgagcttgcg ttggagttcc tggcttccca tagcgatacc tttttcttgt
    44221 ccaatacgtt gtaggggaga gtttgcagcc atttaaaagg taacatgaca atgcatgatg
    44281 aatataacaa catattatgt tatgtgatgt ggaggaagat cacaaaattt cgcacaggat
    44341 cgcgctgtgg ctaatggatg tagttataaa attgaattta aagtgaaaat atttttacgg
    44401 gcgggggcaa gaaggacata gaaacaaata cgccctcgga aaatccagag ggcgtcgggc
    44461 aattaaaccg gtgttagccg atttctgtca gagacttact gtgcagtagg accgcaaccg
    44521 ccaatgattt tagaaataga aattgccggg tgcagcaggt agtcgtaaga gcagttctta
    44581 tcacggtttt caatatgacc agtacatgca gttaacgtag ccagtactgc acctaccagt
    44641 gcaactttaa taaatttgtt catttacatt ccctatgtat tatatattaa ttaatattca
    44701 ttgattatat gaatataagc agactgctcg ttaagaacga tacaggaata tttaattggc
    44761 gtcagtgagc gaatgactta attttgtaaa tcgctgtaac ggtgaatata acccattagc
    44821 agataatatt tattaagggg ttatgtttta tttgctgaat atataaaaag agtcattaag
    44881 aaggccagaa ttctctggcc cgggttaaat taatgattgg agaaccagtt cagtttatcg
    44941 cgcaggccaa caacccgacc aataataatt agcgatggac tgttcatttg ctgagcaagt
    45001 tcaccgagtt gcgtgagcgt accgtcaatc acgcgctgcg tgactgccgt tccgttttcg
    45061 acaattgcca ccggcatttc gccaggcata ccgtgttcaa tcagcttttg ctgaatagtc
    45121 gcggcctgat tcaaccccat atagaacacc agcgtctgtt tttctgccgc caggttttcc
    45181 cagtccagct cgccaccggt ttttaagtgt ccggtaatta agcgtacgct ctgggcataa
    45241 tcgcgatgcg tgagtggaat acccgaatag gcagagcaac cagaagctgc ggtaataccc
    45301 ggaaccaccg agaacggaat acccgcgttg cacagtgttt ccagctcttc gccaccacgg
    45361 ccaaaaataa acggatcgcc acctttcagc cgcaccacgc gtttgccttt ttgcgcttcc
    45421 cgcagcagga tctggttaat ctcttcctgc ggtacacagt ggtatcccgc gcgtttgccg
    45481 acgaaaacgc gatcagcatc gcggcgtacc agattcataa tatcgtcaga aaccagacgg
    45541 tcgtagacca ccacatctgc ctgctgaatt tgttgcagtc ctttcagcgt cagcagcccg
    45601 gcatcgcccg gacctgcacc aaccagcacc acttcaccgc gatggtcgag cggttcgttg
    45661 attaactgtt cggtcgtttc agtaatggct ttctgatcgt tgtttgccag cgactgcgcc
    45721 aggcggtcgt taacgaacaa tttctcccag aaacggcgac gctcactcat cgtggcgaac
    45781 tgttgtttca ctcgcccgcg taattgcccg gcatatttcg ccacctggcc cagatgcaac
    45841 ggcagcagtg attcgagttt ttcgcgcaac aggcgcgcca gaaccggaga ggtgccgcca
    45901 gaggagaccg ctaccattag cggtgagcgg tcaataatcg acggcataat aaagctggcg
    45961 gctttcggcg catcaactac gttacagaag atgcgacgag cttcagcggc ttcgctgacg
    46021 cgctggttaa gcgtgtcatc atccgtcgct gcaatcgcca gccagcaggt gtcgagaagg
    46081 ctttcatcaa atggcccttc gacgagggtt aacatgcctg catctgccca tgcggtgaac
    46141 tgtggaataa acgctaatgc attcaccgtt aagcgagcgc ctgcgtctaa cagcaacctt
    46201 gctttgcgtt ccgcgacatc accaccgccg acaatcagac agtcgcgatc gcgtaattgg
    46261 caaaatatag gcaaatgatc cacgtaaaaa ccccttagta attaaccggc agctgtttca
    46321 gtttgattaa atttgtccgc aaccggacga ttcgctttcg gcgtagcata ccaataaccc
    46381 aatcccatga atacggcacc tgataaagta ttacccagcg tcacccacag caggttatga
    46441 ccaatacccg ccagcgtgta ggcttcgctg tggttgccga accaggagag cgcgaacagc
    46501 gtcatgttag cgatagagtg ctcgtagccg gacgcgataa atgccagcag acaccaccag
    46561 atagcgataa atttcgccgc cccttcagtg cgcagcgcca tccagattgc caggcaaacc
    46621 agccagttac acaatgcacc tttgaagaac agtaccattg ccggtgcagt ggttttagcc
    46681 agcgcgacgg agtgaacgat gctggtatct accggcagca ggctaccgcc gccccagcta
    46741 tagagcatgg caacgaagac ggaaccgacc aggttaccca gccaggtttg cggcaggatt
    46801 gcccacattt gcccgtggct gatgctgccc gcttttaccc caaaggtgag gaacatggtg
    46861 tgtccggtga acagttcaga accggcgata atcaccagcg ttaaggcgat accaaaggtc
    46921 gcgcccatca ccagcggacg tacggacggg tcgagcaaat taccgagcgt gaaaatcagg
    46981 atgatcccaa gacccacata tgcgcccgcc atggcggagc tgacccaaaa gccgagcggg
    47041 ttatttgccg acaggcgtgc aatgcgcgca gcattagccg cacacttatt aatagtgtct
    47101 gtaaacattt gattatcctt taaaaaataa aaacaaaaaa taaaacaaaa agtttaaaaa
    47161 atgtcctgca atataccgaa ggtcattaca ggaccatgca aaaaggggag gcattgcgcc
    47221 tcccgttaaa acattaaccg cgcagctgca ccacgccgtc tttcactcgt gcttcgtaat
    47281 gtttgacgga aaactgttcg tcttccatgc acaagccatc acttaagcga aaacgctgtt
    47341 ttttcagcgg gctggcgacc cacagctcgc cctggtgttc cgcaatcagc ccgcgtgata
    47401 gcacgctgga ctcgaagaac ggatcaatgt tgctgatcgc aaacacctgg tcgctgtgat
    47461 acgggcggaa aatcgctacc tgcttgtcac ctaacagtgc gcagacgccg gtttcaggca
    47521 ggatgtcatc gattttgcag atgtctttcc actggctcat gcgttgtcct ccaccagagt
    47581 taccgggata cgttcatacg gcgttgccgg acggtgctgt tcgcgctctg gcaccatctg
    47641 cacgttcggg tcacgcttgt cgctgttgat gaagtgtttg aagcgagtct gcgcggacgg
    47701 tgtattgacc gtttcagtcc actcacacac taccgcttca cgcaggcgcg ccatctcttc
    47761 ttccagatga gcgttcagcc ccagtttgtc gtcaatgatc accgctttca ggtaatcgat
    47821 gccgccttcg aggttttcta accacggcgc ggtacgcgtc agtttgtcgg cggtacggat
    47881 gtagaacatc atgaagcggt cgagatattt gatcagcgtt tcgcgatcga tatccgctgc
    47941 cagcagatcc gcatgacgcg gtttcatgcc gccgttaccg caaacataca ggttccagcc
    48001 tttttcagtg gcgataatac ccacgtcttt accctgagct tctgaacatt cacgggtaca
    48061 gccggagaca ccgaacttca ttttgtgcgg cgtacggatg cctttgtagc ggttttccag
    48121 ttccacgccg aggccgacgc tatcgccaac accgtagcgg caccaggtgc tacccacgca
    48181 ggttttcgcc atacgcagtg ctttcgcata ggcatgaccg gtttcgaagc cagcttcaat
    48241 cagctgacgc cagatttccg gcagatcgtc tttctgtgcg ccaaacatcg ccaggcgctg
    48301 ggagccggtg attttggtgt agagattaaa ttcacgcgcg atacgaccca ctgccatcag
    48361 cccttccggg gtgatttcac cgcccggaga acgcgggatc accgagtagg tgccgtcttt
    48421 ctggatgtta gcgaggaagt tgtcgttaga atcctgcagc ggagtatgtt ccggcttcag
    48481 aatgtattcg ttccagcagg aggccagcag cgaaccgacg gttggtttac aaacttcaca
    48541 accgtagcct ttgccgtgtt tcgccagcag ttcttcgaag gttttaatgc cttcaacgcg
    48601 gatcaaatgg aacagttcct gacgcgaata agcaaagtgt tcgcacaggt tgttgttaac
    48661 ttcgatgccc tgtttcgcca gttccgcgtt cagtacctga gtgaccagcg ggatacagcc
    48721 accgcagcca gtacccgctt tggtttcagc tttcagcgcc gcaactgtgt ggcagccttt
    48781 gttgatggca gcaatcagat cacctttagt gacgtcgaag caggagcaga tttgcgcgct
    48841 gtccggcagt ttatcaacac cgatagacgg cttaccgcta cccgagtgcg ccggcagaat
    48901 cagagaatcc gggttttccg gcagctcgat agcgttcagc accagttgca gcaggttgcc
    48961 gtagtcgctg gtatcgccca ccagtaccgc accgagcagg gttttgttgt cttcgctgac
    49021 aatcaggcgt ttgtagatct ctttactttc gtcgaggtaa acatagctac gtgcgccagg
    49081 cgtgcgaccg tgcgcatcac caataccacc tacgtctacg cccagcagtt tcagcttggc
    49141 gctaaggtca gcaccttcaa aggcgttttc gctacccaga atatggtcaa cggcgacctg
    49201 cgccattttg tagcctggtg ctaccagacc aaatacacgg ttgttccagc ttgcgcattc
    49261 accgatggcg tagatatccg gatcggaagt ctggcaggaa tcattaatga caatcccccc
    49321 acgcggagca acgtccagac cacactgggt tgccagctta tcgcgcggac ggataccggt
    49381 agagaagacg ataaagtcga cttccagttc gctgccgtcg gcaaaacgca tggttttacg
    49441 cgcttcgaca ccttcctgca caatctcaag ggtgtttttg ctggtgtgaa cgcgcacgcc
    49501 catactttcg attttgcgac gcagctgctc gccacccatc tgatcaagct gttccgccat
    49561 cagcataggg gcaaattcga taacgtgggt ttcaatacct aagtttttca gcgcgccagc
    49621 ggcttccaga cctaacaggc cgccaccaac aacggcaccg cgtttgctgc gacgggcgca
    49681 ggattcaatg gcgttgaggt cttcaatggt gcgatagaca aagcagtcct gagtatcaga
    49741 acctttgatt ggtgggatcc acgggtagga accggttgcc atgatcagct tgtcataaaa
    49801 aacggtacgt ccggcgctgg agtgaatcac cttctcctga cggttgatgg tgatagcgcg
    49861 ttcgccgacc agaactttga tgccgtgttt ctcgtagaag ccttcgcgca ccagcgacag
    49921 ctcttcggcg gtgtggtgag agaagtaaga tgagaggtgt acgcggtcat aagcgatgcg
    49981 cggttcttca cagaaaacgg taatatcaaa gttggccgca tcagatttat caagaagatc
    50041 ttcgataaag cgatggccga ccataccgtt accgataatt gcgagtctga ctttgctcat
    50101 ttttgcctcg atttcttttc tattaccgcc taccttaacg attcagcaac cccgcttatt
    50161 gatgtaaatc aaattcacct ttatatactc cttaaggggt atattgctga tttgtataaa
    50221 ttttcttaag tcacggaatt gtctgacatt tcatgtttgt gcaaaaaatg gacaaaatga
    50281 acgggtttat agaaatagat gcacgtagcg ggggaagaga ggaaaaggcc ggacgacgat
    50341 agtgccgtcc ggcattagca attaatgaga ggtcagggtg ttatgctgac ggtgacggct
    50401 gacgaaacct aacaggaagc acatcacaaa gacgacagcg tacagaccgt ttgctgtaag
    50461 cagtgccgcc tgcggaccgc tatgttcaac aatcgtgccg gtaaccacaa aggtcaacat
    50521 agtaccgatg gttccgcagg tcaggacaaa gttaaccagt tttggcgacg gtactttggt
    50581 ctgctgtgaa cccagggtga tgatggtggt atagatcgcg ctggagaaga agcccagagc
    50641 gagaattgac cacgccatat gtactggtgt tccggtgtta aagacgtaca tcagaatcgc
    50701 agccagacca gccagtacgg tcagaatgcg ttgcaaatca aagaagcgaa gaataaagct
    50761 gaacgcccac atgccgacca tgtatgacat ccagaagtta ctcaccagcg tgcccgcgtc
    50821 gttcaggctc atgcccaggc ctttggcata ctcaggcacc caggagataa aacctaactg
    50881 accgaggatg tagcacagcg ccgcaacaga gagaaacagt acgccgatcc cccacttttc
    50941 tttctctacc ggagcatccg tttttggtgc acgtttgccc agtgccggga actcacagcc
    51001 gaaggtcaga ataaaaatag cgacatacac cagcccgatg caggcataaa cccagtacca
    51061 ctcaatgctg cgcgccagta gaaacgcggc gatcattggg aaaatcatcc cagccatact
    51121 gaagaaggag tcagtaaata acaggcggga accgcgctga cgcccttcat acatttgtgt
    51181 tatcaggaat gtaccaatcg acatagtaat gccgctgacc accccgagaa tgaacatcgc
    51241 cgtcgagaac agcgccaggc tgtggctgaa catcaaaccg gcaaccgcca gcaccatcag
    51301 gagaaagcca aaacgtaact gcgttttcaa cgggacgatt tccatcagcc aggcgttgag
    51361 gaagatagag attaaaatgc cggcgttgag gaaggtgaag gtgttactca tactggaaac
    51421 aggcagattg aaataatcgg cgatatttcc catcaccatc ccggtgacaa taaccaacgc
    51481 accagtcagt gcgtaggaga gaaagctaat ccatgtgagc ttgatgcgat tgctgttagt
    51541 catgattggc ctgcgttcaa aaataaaatg gcatagcggg atatgccgcg agcgggcgat
    51601 tttaggtgat tttatgatct gtttaaatgt tttattgcaa tcggttgcta aattgcattt
    51661 taagaggtga ttttgatcac ggaataaaaa gtgatcgtca ggttacatat atttcagata
    51721 cgtaaaatta ggtaaaggga tggccttgtt cttgaaggct atttagaatc tcttcacttg
    51781 ctttttttct gctctgtttg ttaaggaaat ctcatgttca aatcgaccct ggcggcgatg
    51841 gctgctgttt tcgctctttc tgctctttct gctctttctc ccgcagcaat ggcagcgaaa
    51901 ggggacccgc acgtattgtt gacaacctca gctggtaaca tcgaactgga gctggataaa
    51961 caaaaagcgc cagtgtctgt gcaaaacttt gtcgattatg tgaacagcgg tttttataac
    52021 aacactacct ttcaccgcgt cattcctggc tttatgattc agggcggcgg tttcaccgag
    52081 cagatgcagc agaaaaaacc aaacccgcca atcaaaaatg aagccgataa cggcctgcgc
    52141 aacacgcgtg gcaccatcgc gatggcgcgt accgctgaca aagacagcgc caccagccag
    52201 ttctttatca acgttgccga taacgccttc cttgaccatg gtcagcgtga tttcggttac
    52261 gcggtatttg gtaaagtggt gaaaggcatg gacgttgccg ataagatttc ccaggtgccg
    52321 actcatgatg ttggtccgta ccagaatgtg ccgtcaaaac cggtagttat cctttccgct
    52381 aaagtcctgc cgtaatgatt tctcgcgcgg gcaatcttgc ccgcgcttct gctctcccgg
    52441 cgtaacccgg atttgccgct tatacttgtg gcaaatggac acgttcaggg aggcatcaag
    52501 tgaagaaact caccgataag caaaagtccc gtctctggga gcttcagcgt aatcgtaatt
    52561 tccaggccag tcgccgtctt gaaggcgtcg agatgccttt agtcactctt actgccgcag
    52621 aggctttagc gcgccttgaa gagctgagga ggcactatga gcgataaatt cggcgaaggg
    52681 cgcgatctgt atctttatcc tggccttgat atcatgcgta accggctgaa catccgccag
    52741 cagcagcggc tggaacaggc cgcttacgaa atgacggcgc tgcgtgctgc gaccattgag
    52801 cttggtccgc tggtgcgcgg tttaccgcat ttgcgcacta tccatcgcca gctgtactag
    52861 gatattttcg actgggcagg gcaactgcgt gaagttgata tttatcaggg cgatacgccg
    52921 ttctgccact ttgcttatat cgaaaaagag ggcaatgccc tgatgcagga tctggaggaa
    52981 gaaggttatc tggttggcct ggagaaagcg aagttcgtcg agcggctggc gcattactat
    53041 tgtgaaatca acgtgctgca tcccttccgg gtgggaagtg gtctggcaca gcggatcttc
    53101 ttcgagcaac tggcgattca tgccggatat caactgagct ggcagggtat cgaaaaagag
    53161 gcctggaatc aggcaaatca gagtggggca atgggggatc tcaccgcact gcagatgata
    53221 tttagcaaag tggtaagcga agccggggaa tctgagtaaa atagcgcggt tctttcgtac
    53281 cggagccgcc atgatcctgc ttatagataa ctacgattct tttacctgga acctctacca
    53341 gtacttttgt gaactggggg cggatgtgct ggttaagcgc aacgatgcgt tgacgctggc
    53401 ggatatcgac gcccttaaac cacaaaaaat tgtcatctca cctggcccct gtacgccaga
    53461 tgaagccggg atctcccttg acgttattcg ccactatgcc gggcgcttgc cgattcttgg
    53521 cgtctgcctc ggtcatcagg caatggcgca ggcatttggc ggcaaagttg tgcgcgccgc
    53581 aaaggtcatg cacggcaaaa cctcgccgat tacacatagc ggtgagggcg tatttcgggg
    53641 gctggcaaat ccacttaccg tgacacgcta ccattcgctg gtggtggaac ctgactcatt
    53701 accagcgtgc tttgacgtga cggcctggag cgaaacccgc gagattatgg ggattcgcca
    53761 tcgccagtgg gatctggaag gtgtgcagtt ccatccagaa agtattctta gcgaacaagg
    53821 acatcaactg ctggctaatt tcctgcatct ctgatttccg attgccattc agtgattttt
    53881 tatgcatatt ttgtgattat aatttcacat ttatttatgc gtaacagggt gatcatgaga
    53941 tggcaattga acaaacagca attacacgcg cgactttcga tgaagtgatc ctgccgattt
    54001 atgctccggc agagtttatt ccggtaaaag gtcagggcag ccgaatctgg gatcagcaag
    54061 gcaaggagta tgtcgatttc gcgggtggca ttgcagttac ggcgttgggc cattgccatc
    54121 ctgcgctggt gaacgcgtta aaaacccagg gcgaaactct gtggcatatc agtaacgttt
    54181 tcaccaatga accggcgctg cgtcttgggc gcaaactgat tgaggcgacg tttgccgaac
    54241 gcgtggtgtt tatgaactcc ggcacggaag ctaacgaaac cgcctttaaa ctggcacgcc
    54301 attacgcctg tgtgcgtcat agcccgttca aaaccaaaat tattgccttc cataacgctt
    54361 ttcatggtcg ctcgctgttt accgtttcgg tgggtgggca gccaaaatat tccgacggct
    54421 ttgggccgaa accggcagac atcatccacg ttccctttaa cgatctccat gcagtgaaag
    54481 cggtgatgga tgatcacacc tgtgcggtgg tggttgagcc gatccagggc gagggcggtg
    54541 tgacggcagc gacgccagag tttttgcagg gcttgcgcga gctgtgcgat caacatcagg
    54601 cattattggt gtttgatgaa gtgcagtgcg ggatggggcg gactggcgat ttgtttgctt
    54661 acaggtacta cggtgtgacg ccggatattc tgacttctgc gaaagcgtta ggcggcggct
    54721 tcccgattag cgccatgctg accacggcgg aaattgcttc tgcgtttcat cctggttctc
    54781 acggttccac ctacggcggt aatcctctgg cctgtgcagt agcgggcgcg gcgtttgata
    54841 tcatcaatac ccctgaagtg ctggaaggca ttcaggcgaa acgccagcgt tttgttgacc
    54901 atttgcagaa gatcgatcag cagtacgatg tgtttagcga tattcgcggt atggggctgt
    54961 taattggcgc ggagctgaaa ccacactaca aaggtcgggc gcgtgatttc ctgtatgcgg
    55021 gtgcagaggt tggcgtaatg gtgctgaatg ccggaccgga tgtgatgcgt ttagcaccgt
    55081 cgctggtggt ggaagatgcg gatatcgatg aagggatgca acgtttcgcc cacgcggtgg
    55141 cgaaggtggt tggggcgtaa ataagacgta tcaggcaaaa tatgtcggat gcggcgtgaa
    55201 tgccttatcc gatggtttag cctgtattac gccttcgaat cccgcaactt gcgactcagc
    55261 caaatcccgt gatgcggtcg ctgacgccat gccatcgacg aaatggtgtg catggtgttc
    55321 agatggccaa taacccgctg taaatgctgc tccagcgtac ctgccgcgcc ttcgtgcggc
    55381 tgcatctccg gcgcatccat gatattggca tcgccagaac tacccggttc gtcatactcc
    55441 agtcgctgct gacaacgctg aatggcgatt tcacaagact gtaaatactc ttgtgccagt
    55501 tcaggtggca atgcccggtg ttcccgcgcc agcgtggtca tggcattaat atgctcaaca
    55561 ataaactggc tgtgcgttac ccacagtttc atatctgcca gataatggct gttgaatgcc
    55621 ggttcctgca tcgcctgatt caatgagtta tacagagtgt tatgcgcctg atttacccgc
    55681 attctctgcc aggccagtgg cgttggttgc gggtcctcgc tcagaatcaa gcgaatcgct
    55741 tcctgatagg cttctaaagc atcatgggcg tttttacgca ataacccgct ctgccactgc
    55801 ggccacagcc agacggtacc accgaaagca atcaaacaac caataatggt atcgataaga
    55861 cgtggaagga tgtactgctc accgttcagc cacaacagtt gcagggtata cactgcggta
    55921 atagtaaaac cgaccgttgc ccagccgtag tttttgcgca atatcaggta gctggcgagg
    55981 gtggtaatca gcatcaacgt cagggtgtaa ctttcgggaa ttttaaagtg cagcgctacg
    56041 cccgcaatga ttaacccgac cacggttccc acggagcgat tcacaatcct cagacgggtt
    56101 gcgccatagc cattttgtgt caccagcaat accgtcatca ggatccagta cgacttcggc
    56161 agatgcagca cggtgcccat caggctggca acgcttaaca tcacactgag tcgtccggca
    56221 ttgcgtagcg ccggagactt tagtgacaga taacttttca gtgccggaag caacggcatt
    56281 cgccgctgtt tatcggccag taagtcacgg gcatagagcg gtttttgggt gcgcagcacg
    56341 cgggcgatgc ggctgaaatg ccagtagcag aattgcccaa ccggattatc cggatgctgg
    56401 cgggcgattt tttccagtgc gccaatttgc ttttccatcg taaaacgcgt tggcaggcga
    56461 tggtaaagaa tgtcatcagc cagcacgcgc aggcgagcgg cgacggtttg cgcattccag
    56521 cggataactt cttccgcatg gctacgctcg accagttttt gcacctcttc cggctgatgc
    56581 aaactgaccg aaatatgttc ctgcaaatcc agcgcctcct ggaaaatacg cagcatccgc
    56641 ttgtaatcgg tattgttttg cgcggaaagc atatgcattt gctgatagca ctgagtaatt
    56701 aaatcgaccg ctttttgctg gcgcaccagc agcggtggca gcgctttttc aggatcggtg
    56761 tgctgggtaa gcaggctgta tttggcttca caataatctg ccagttcacg gtacagcaga
    56821 cttagtgact cgcgcagcgg ttgttcgcgc cagatccaga accaaaacca gttaaacaat
    56881 ccgtaccaga gagtgcccaa cgcatagatg agcaatggtt cccagaccgg catgtatccc
    56941 gccagactga gggtaaaaat ggcggcgagc agcgatgcag gaagcaattt tgcgtgtaat
    57001 ggccccagct cagcagtgac gccaagtacc agcgttaatc cggtcagcaa aaagggcagg
    57061 ggaacatctt ttaccagtag cagctgtgtc agtaagctac aggtggcaaa cagcgacgca
    57121 ccaatgatta agtgtttgaa aaaacgttta tgaggcgtat caaggcccgc aatattgcaa
    57181 caggcaggaa cgagggagaa gagcagaccg aatcgtaatt cgccaagcat taacccaacg
    57241 gccacgggca aacatagcac cagcgtttgt cgaagtgcat agttgatatc ggggtgataa
    57301 atcagtctgc gccacatcgg gggaaacaaa atggcgcgct accaggtaac gcgccactcc
    57361 gacgggatta acgagtgccg taaacgacga tggttttacc gtgtgcggag atcaggttct
    57421 gatcttccag catcttcaga atgcgtccca cggtttcacg agaacagccg acaatctgac
    57481 cgatttcctg acgggtaatt ttgatttgca taccgtccgg gtgagtcata gcatctggtt
    57541 gttttgccag gttcagcaga gtctgtgcaa tgcggcccgt cacgtcgagg aacgccaggt
    57601 tgcccacttt ctctgaagtg acttgcagac gacgcgccat ctgtgcagac agacgcatca
    57661 gaatgtccgg gtttacctga atcaattggc gaaatttttt gtacgaaatt tcagccactt
    57721 cacaggcggt tttcgcacgt acccatgcgc tacgttcctg gccctcttca aacaggccca
    57781 gttcgccaat aaaatcaccc tgattcagat aggagaggat catttcttta ccctcttcgt
    57841 ctttgatcag cactgccaca gagcctttaa cgatgtagta cagcgtttcc gctttttcac
    57901 cctggtgaat aagcgtgctc ttggatgggt acttatgaat gtggcaatga gacaagaacc
    57961 attcgagagt cgggtctgtt tgcggtttgc caagcaccat gcgcggttat cctctgttat
    58021 aagctttctc cagagccaga taacgccgct gtctctggat tgccgaaata tgcttcccgc
    58081 tacctgggaa ggggctatca actgtactgc acggtaatgt gacgtccttt gcatacatgc
    58141 agtacatcaa tgtattactg tagcatcctg actgttttag catagctttc gctttgtgtc
    58201 tcctggtgtc tcgcttcagc atgacccagg tcgccttccg ttgcgcgatt tggttagtac
    58261 gcgtactctg tcaggaaaat tgacgcagtg gagtagcaaa aatgcaagcg cgagtgaagt
    58321 gggtcgaagg gttaactttt ctgggcgaat ccgcctctgg tcaccagatt ttaatggacg
    58381 gcaactcagg cgataaagca ccaagtccga tggaaatggt gttgatggcg gcgggtggtt
    58441 gcagtgccat cgatgtggtt tcgatcctgc aaaaagggcg tcaggatgtg gtcgattgtg
    58501 aagtaaaatt gacctctgaa cgccgcgaag aggcaccacg cctgtttacg cacattaatc
    58561 tgcattttat cgtcaccggt cgcgacctga aagacgcagc ggttgcgcgt gcggttgatc
    58621 tctctgccga gaaatattgt tcagtggcgt tgatgctgga aaaagcggtg aatattactc
    58681 actcgtatga agtggttgcc gcgtgactta actatcccgt tgtgaagccg gatagtattt
    58741 tatccggcat aatagcaaag ttactcgatt ttctttcctt ccatcaatcg ttgcaccagc
    58801 ggcagcataa ttaattccat tgccagcccc attttgccgc caggtaccac taacgtattg
    58861 atgtgggaaa tgaatgagcc ttgcaacatc gccagcagcc aagggaaatc gatcccttcc
    58921 agattacgaa aatggatcac cacaaagctt tcatccagcg acgggatacc ttttgccgcg
    58981 aacgggtttg aagtgtcgac ggtgggaacg cgctggaagt taagatgggt gcgggaaaac
    59041 tgcggtgtga tgtagttgat atagtcttcc attgaacgca ctaccgagtc catcactgct
    59101 tctcgtgagt gcccgcgctc gctggtgtcg cggatcagtt tttgaatcca ctcaaggtta
    59161 acgataggca ctacgccgac cagtaagtcc acatgctgcg caacgttatg ctgtggcgtg
    59221 actacgccgc cgtgtaaacc ttcataaaac agtacatcag tgggttctgg taaaggttgc
    59281 cagggggtga atgttcccgg tacctgattc cagggtacgg cttcgtcgta ggtatgcaga
    59341 tatttgcgag atttcccttt gccgctctga ccgtattcaa tgaaggtttg ttccagcagg
    59401 ccgaagtcgt tcgcttcggg gccgaagtag ctgatatgcc gcccggcgtc gcgcgctttg
    59461 cggatcgcca tgtccatttc cgggcgggtg taacggtgaa aactgtcgcc ttccacctca
    59521 gctgcatgca gatttaactg cgcgaatatt ttacgaaacg cgaggctggt ggtggtggtc
    59581 cccgcgccgc tggatcctgt taccgcaatg accggatgtt tggcagacat agtaactcca
    59641 tgacttagct atcactatta tctcgtccgc cgacggttgc aggttattcg cgaaactgac
    59701 tgcgcggcat aatattgacc gtttcgtgca gctccgacca taccagcacc gcttctccgc
    59761 actgtagctg gcgtttgacg tcggcgactt tctgttcaag tgtacgctca tgttcaccat
    59821 aatcggtgcc ttcacgtaac acaaagcttt caattaaatt ttccagcgtt tcgggggaga
    59881 ggtcttgcca cggaatcaac atgatttcgc ctccagatac gttgttaacc agtcaggaat
    59941 gcgtgactcc agccacattt gcggatgaag taatgtaccg ccaataaagc caacatgacc
    60001 gccatgttca gtcagttgat actccacctg cgaggggaga ctttccggtt ttgggatcac
    60061 ctgatgatcc ataaacggat cgtctttggc gtgaataatc agcgtcggtt tggcgatccg
    60121 gttcagcatt ggcatggcgc tacactgacg ataatagtcg atagcgtcgg cgtagccgtg
    60181 aattctggcg gtgattagat cgtcaaattc acggatgcga cgtaccgatt ttaactgcgc
    60241 gagattaatc ggcagcgttc cggggtaggc tgccagcttg cgcgcggcat tggcttttaa
    60301 caggttcagc aagtaacgct gataaacgc
//
LOCUS       DAJTSI010000006        58030 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000006 DAJTSI010000000
VERSION     DAJTSI010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58030
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.006"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(24..278)
                     /locus_tag="OO327_000312"
     CDS             complement(24..278)
                     /locus_tag="OO327_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR6016990.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="OO327_000313"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="OO327_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR6016991.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="OO327_000314"
     CDS             1228..2694
                     /locus_tag="OO327_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR6016992.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /gene="yeiR"
                     /locus_tag="OO327_000315"
     CDS             2812..3798
                     /gene="yeiR"
                     /locus_tag="OO327_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR6016993.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="OO327_000316"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="OO327_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR6016994.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="OO327_000317"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="OO327_000317"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR6016995.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="OO327_000318"
     CDS             5709..7265
                     /locus_tag="OO327_000318"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR6016996.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVHETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="OO327_000319"
     CDS             7347..9161
                     /locus_tag="OO327_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6016997.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="OO327_000320"
     CDS             9162..10256
                     /locus_tag="OO327_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR6016998.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="OO327_000321"
     CDS             10256..11281
                     /locus_tag="OO327_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6016999.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="OO327_000322"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="OO327_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR6017000.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="OO327_000323"
     CDS             complement(12876..13220)
                     /locus_tag="OO327_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6017001.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="OO327_000324"
     CDS             complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="OO327_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCR6017002.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="OO327_000325"
     CDS             complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="OO327_000325"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR6017003.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15616..17376
                     /locus_tag="OO327_000326"
     CDS             15616..17376
                     /locus_tag="OO327_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR6017004.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17501..17785
                     /gene="rplY"
                     /locus_tag="OO327_000327"
     CDS             17501..17785
                     /gene="rplY"
                     /locus_tag="OO327_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR6017005.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="OO327_000328"
     CDS             complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="OO327_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR6017006.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19113..19340
                     /locus_tag="OO327_000329"
     CDS             19113..19340
                     /locus_tag="OO327_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR6017007.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19360..21120
                     /gene="pbgA"
                     /locus_tag="OO327_000330"
     CDS             19360..21120
                     /gene="pbgA"
                     /locus_tag="OO327_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR6017008.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21195..21271
                     /locus_tag="OO327_000331"
     tRNA            21195..21271
                     /locus_tag="OO327_000331"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21229..21231,aa:Pro,seq:ggg)
     gene            complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="OO327_000332"
     CDS             complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="OO327_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCR6017009.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKTGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAVAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24285..24932
                     /gene="narP"
                     /locus_tag="OO327_000333"
     CDS             24285..24932
                     /gene="narP"
                     /locus_tag="OO327_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR6017010.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="OO327_000334"
     CDS             complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="OO327_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR6017011.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="OO327_000335"
     CDS             complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="OO327_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR6017012.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="OO327_000336"
     CDS             complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="OO327_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR6017013.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="OO327_000337"
     CDS             complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="OO327_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR6017014.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="OO327_000338"
     CDS             complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="OO327_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR6017015.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="OO327_000339"
     CDS             complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="OO327_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR6017016.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     IMVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="OO327_000340"
     CDS             complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="OO327_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR6017017.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="OO327_000341"
     CDS             complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="OO327_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR6017018.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31442..32044)
                     /gene="napC"
                     /locus_tag="OO327_000342"
     CDS             complement(31442..32044)
                     /gene="napC"
                     /locus_tag="OO327_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR6017019.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32054..32503)
                     /gene="napB"
                     /locus_tag="OO327_000343"
     CDS             complement(32054..32503)
                     /gene="napB"
                     /locus_tag="OO327_000343"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR6017020.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32500..33363)
                     /gene="napH"
                     /locus_tag="OO327_000344"
     CDS             complement(32500..33363)
                     /gene="napH"
                     /locus_tag="OO327_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR6017021.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33350..34045)
                     /gene="napG"
                     /locus_tag="OO327_000345"
     CDS             complement(33350..34045)
                     /gene="napG"
                     /locus_tag="OO327_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR6017022.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34052..36538)
                     /gene="napA"
                     /locus_tag="OO327_000346"
     CDS             complement(34052..36538)
                     /gene="napA"
                     /locus_tag="OO327_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR6017023.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36535..36798)
                     /gene="napD"
                     /locus_tag="OO327_000347"
     CDS             complement(36535..36798)
                     /gene="napD"
                     /locus_tag="OO327_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR6017024.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36788..37282)
                     /gene="napF"
                     /locus_tag="OO327_000348"
     CDS             complement(36788..37282)
                     /gene="napF"
                     /locus_tag="OO327_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR6017025.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37391..37555
                     /locus_tag="OO327_000349"
     CDS             37391..37555
                     /locus_tag="OO327_000349"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6017026.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            37690..38178
                     /gene="eco"
                     /locus_tag="OO327_000350"
     CDS             37690..38178
                     /gene="eco"
                     /locus_tag="OO327_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR6017027.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38300..39331)
                     /locus_tag="OO327_000351"
     CDS             complement(38300..39331)
                     /locus_tag="OO327_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HCR6017028.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(39565..39924)
                     /locus_tag="OO327_000352"
                     /pseudo
     CDS             complement(39565..39924)
                     /locus_tag="OO327_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(40142..41785)
                     /gene="yojI"
                     /locus_tag="OO327_000353"
     CDS             complement(40142..41785)
                     /gene="yojI"
                     /locus_tag="OO327_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR6017029.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41861..42511)
                     /gene="alkB"
                     /locus_tag="OO327_000354"
     CDS             complement(41861..42511)
                     /gene="alkB"
                     /locus_tag="OO327_000354"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCR6017030.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(42511..43575)
                     /gene="ada"
                     /locus_tag="OO327_000355"
     CDS             complement(42511..43575)
                     /gene="ada"
                     /locus_tag="OO327_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR6017031.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43649..44704)
                     /gene="apbE"
                     /locus_tag="OO327_000356"
     CDS             complement(43649..44704)
                     /gene="apbE"
                     /locus_tag="OO327_000356"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR6017032.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44816..45937)
                     /gene="ompC"
                     /locus_tag="OO327_000357"
     CDS             complement(44816..45937)
                     /gene="ompC"
                     /locus_tag="OO327_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6017033.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46675..49347
                     /gene="rcsD"
                     /locus_tag="OO327_000358"
     CDS             46675..49347
                     /gene="rcsD"
                     /locus_tag="OO327_000358"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR6017034.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            49364..50014
                     /gene="rcsB"
                     /locus_tag="OO327_000359"
     CDS             49364..50014
                     /gene="rcsB"
                     /locus_tag="OO327_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR6017035.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50214..53063)
                     /gene="rcsC"
                     /locus_tag="OO327_000360"
     CDS             complement(50214..53063)
                     /gene="rcsC"
                     /locus_tag="OO327_000360"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR6017036.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53338..54114)
                     /locus_tag="OO327_000361"
     CDS             complement(53338..54114)
                     /locus_tag="OO327_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCR6017037.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54119..55768)
                     /locus_tag="OO327_000362"
     CDS             complement(54119..55768)
                     /locus_tag="OO327_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR6017038.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55769..56328)
                     /locus_tag="OO327_000363"
                     /pseudo
     CDS             complement(55769..56328)
                     /locus_tag="OO327_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            complement(56332..56490)
                     /locus_tag="OO327_000364"
     CDS             complement(56332..56490)
                     /locus_tag="OO327_000364"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6017039.1"
                     /translation="MTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGG
                     GEDWRWVG"
     gene            complement(56487..>58030)
                     /locus_tag="OO327_000365"
     CDS             complement(56487..>58030)
                     /locus_tag="OO327_000365"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR6017040.1"
                     /translation="TSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNN
                     VRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQ
                     GKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKP
                     AAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQN
                     GQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRN
                     NLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGP
                     GARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLL
                     HRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPL
                     ADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTA
                     AILMV"
BASE COUNT        14196 a        15654 c        14258 g        13922 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgcg ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcagccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttatg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactggtta gctgccttca gtttttagtg gcctggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccatgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc gacagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcacccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgact atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcac tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcc tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcgcattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgagg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaacg
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cggcgcactg atgaccatcc
    13861 acgcagccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg tgaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccagcccg tggaagaaac gcatcacaat cagctgatcg atggtttgtg
    14461 ccaacgcaca cgccaccgcg gcggcggcaa acaccagcgt cccgccaaga accaccggct
    14521 tacgcccgaa gctgtctgcc atcggcccgt agattaactg ccccaacgca aagcccagaa
    14581 tataagtact gagggtcatc tgcgtactgc ccgccgatac gccaaactgc gctgaaatta
    14641 ccggtagcgc gggcagatac atatcaatcg acagcggcat caacatggcc agcaggccaa
    14701 ggataaaaac aatagcaaac gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt
    14761 cattgaaaag ttagacgacg ctggcaattt cttcttcagt taacggacga tattcaccgg
    14821 gggctaaatc agcatccagc gtaataccgc caatacgttc acgatgcagc tcaaccacgt
    14881 ggttacccac ggcggcgaac atgcgtttca cctgatgata acgcccttcg ctgatggtca
    14941 gacgaacctg cgttggggta atcacttcaa gcaccgccgg cttagtgaga tctttttcgt
    15001 tatgcagctg cacgccttta gcaaattgct ctgccgtatc gtcagctaca ggtgattcca
    15061 gtgtcaccag ataggtcttc tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact
    15121 gaccatcatc agtcatcagc accagaccgg tggtatcgat atccagacgt cctgcggcgt
    15181 gcagtttcca cgctaccggt tcatccagaa aatagagcac cgtcgggtga tccgggtcgt
    15241 ccgtggagca aacatagccc tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct
    15301 gcgccagcgg gttgccatcg taagcgacat catgttcagg gagcagtttg aacgctgcat
    15361 tacgaacgat ttcgccatcg acggtgacac gattgccgcg gatttcacgc ccggcaatag
    15421 cacggctaac gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc
    15481 ctgtaaaaaa cggaggtcgg gtaatgaccc gaaaatctga actgttgtct gcccagtata
    15541 gcggtctaat aacttccctc aagggaaaaa gaaccgtggc atactattag cagaataatc
    15601 tacctacgcg aaaccatgat ttttacactc cgcccatatc agcaagaagc cgtggatgcc
    15661 acgctcaacc attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggcgca
    15721 ggtaaaagcc tggtgatagc ggaactggca cggctggcgc gtggtcgcgt actggtgctg
    15781 gcacacgtta aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa
    15841 gccgatattt ttgccgccgg gctaaagcgc aaagagagcc acggtaaagt ggtatttggc
    15901 agcgttcagt cggtcgcccg taatcttgat gcctttcagg gtgaattttc gctgttgatt
    15961 gtcgatgaat gccaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact
    16021 cacctgacta aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccttttcga
    16081 ttgggcaaag gctggatcta ccagttccat tatcacggca tggtacgcgg cgatgagaaa
    16141 gcccttttcc gtgactgcat ttatgagctg ccgctgcgtt atatgattaa acacggctat
    16201 ctgacgccgc cagaacgact ggatatgcca gtagtgcaat acgatttcag ccgcttgcag
    16261 gcacagagta acgggctgtt cagcgaagct gatctcaacc gtgagctgaa aaaacagcaa
    16321 cgcattacgc cacatatcat cagccagatc atggagttcg ccgaaaagcg caaaggggta
    16381 atgattttcg ccgccaccgt tgaacacgca aaagagattg tgggattact gcctgccgaa
    16441 gatgctgcac tgattactgg cgacaacccc ggcgctgagc gcgatgtgtt aattgaagat
    16501 tttaaagccc agcgttttcg ctatctggtc aacgtcgcag tactgaccac cggatttgac
    16561 gccccgcacg tcgatcttat cgccattctg cgccctaccg aatcagtgag tctttaccaa
    16621 caaattgtcg ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat
    16681 tatgcgggta atcctcacga tctctacgcg ccggaagttg gtacaccgaa aggcaaaagt
    16741 gacaacgttc cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg
    16801 aaaacgaccg ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt
    16861 gaagatgacg acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg
    16921 caatgtaacg cagaaaatga tattgccgct cgccgctgcc gcgaatgtga cacggtactg
    16981 gttgatccgg atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc
    17041 tgtagcggca tgtctttgca gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc
    17101 tattacgatg aagacggcgc ggatgtgagt gagcgtttcc gtctgcaaac gcccgcccag
    17161 cgtaccgcct tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catccctctg
    17221 cgctggatca ccgccgccga tatcctcgcc cagcaagcct tactgcgaca tccggatttt
    17281 gtcgtcgccc gcatgaaagg tcagtactgg caagtgcgtg aaaaagtgtt cgattacgaa
    17341 ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataag
    17401 gcgattgagt atagaatctc gcccgctttt gcatacgcaa agcagatcac ttacctgttg
    17461 ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga
    17521 agtacgtaaa gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc
    17581 ggcaatcatc tacggtggca aagaagcacc gctggctatc gagctggatc acgacaaagt
    17641 catgaacatg caagctaaag ctgaattcta cagcgaagtt ctaaccatcg ttgttgacgg
    17701 taaagaaatc aaagttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca
    17761 gcacatcgac ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg
    17821 gggttttttg tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga
    17881 taaggcgttc acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg
    17941 tccggcgctg caattggtcg cgcaaattcg gcggcgtgcc tttaatggtc aaggtatcgg
    18001 ttgctggatc ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc
    18061 caccgccgct accggcaaac ttcgtcaact gacgcagtgt gctacggtct gccgggaagc
    18121 tctcttccag ttcgtagcct ttttcagccg caaactccgt gaagctcact tcgcttacgc
    18181 ctgccagctc tttcgacagc gattccagtt cgatctcttc gcctgcctgc agctgctcat
    18241 tacagtagct ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt
    18301 cggcagtgaa atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gcgttcaatc
    18361 cttcgctggc cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt
    18421 tcaggaaagt gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac
    18481 gcgcaacaat atccgcatga ttgatatcaa gataatgggt tgggttgatg tcgagatttt
    18541 cgttaacgcg catactgctc aggttgctca gaactgccac cagcaaatac tccaccgcca
    18601 gataacggta gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt
    18661 cgtcacgcag gcgaccggtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac
    18721 gctgtaagcg cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat
    18781 ttttggcgct atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt
    18841 ccagcaatga atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt
    18901 gcagagcaat ctggttgata tccagactca tgataaactc tcctttaaga ccgggcggta
    18961 ttcaaccacc gccagttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc
    19021 gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg
    19081 ttgccctttc atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtaatg
    19141 aacaggttga acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga
    19201 ccgacctttc cctgatggtg ttaggcaaca tggtcactaa ccttatcaac accagcattg
    19261 ccccggccca acgccaggca attgccaact cttttgccag cgccttacag tcctctatca
    19321 acgaagacaa agcgcactaa gggaaacaga taacaggtta tggtaactca tcgtcagcgc
    19381 taccgtgaaa aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt
    19441 ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt
    19501 gctggtcgca tttattccta cgtaagcatt atcggccatt tcagcttcct agtgttcgcc
    19561 acctacttgc tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg
    19621 tttttgtccg tcattctggc gacggcggga atgacgctat tactgatcga tagcgaagtc
    19681 tttactcgtt tccatctcca tcttaatccc atcgtctggc agttggttat caacccagac
    19741 gaaaatgaga tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg
    19801 cttgaactgg tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga
    19861 cgcttcgcgc gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg
    19921 tatatctggg ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg
    19981 ctttcgtacc cgatgacggc gcgacgtttc cttgagaagc atggcctgct tgatgcgcag
    20041 gagtatcaac gccgtcttat tgagcaaggt aatccagacg ccgtttccgt acagtatccg
    20101 ttaagcgaac tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc
    20161 gatggcctga actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag
    20221 caaaatattt cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt
    20281 ggcctgttct atggcatctc gccgagctat atggacggca ttctgtcgac ccgtacgcct
    20341 gcggcattaa ttactgcgct taatcagcaa ggctatcagc tggggttatt ctcatcagat
    20401 ggctttacca gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta
    20461 cgcacccaat ctgacgatca gaccgccacg cagtggatca actggctggg ccgctacgca
    20521 caagaagata accgctggtt ctcgtgggtt tctttcaatg gcactaacat tgacgacagc
    20581 aatcagcagg catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc
    20641 aaacgcgtgc tcaatgcact gcgtgattct ggcaaactgg acaatacggt ggtgattatc
    20701 actgccggtc ggggtattcc actgagcgaa gaggaagaaa cctttgactg gtcccacggt
    20761 catctgcagg tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgct
    20821 ctgactgatc ataccgatct gatgacgacg ctgatgcaac gcctgctgca tgtcagcaca
    20881 cctgccagcg aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg
    20941 gttactgctg cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg
    21001 aacaataacg gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa
    21061 ccacagttaa gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga
    21121 ttaattataa atcagttagc gaaatatctt acttgcaatc ggtgtggaaa acggtagtat
    21181 tagcagccac gagtcggcac gtagcgcagc ctggtagcgc accgtcatgg ggtgtcgggg
    21241 gtcggaggtt caaatcctct cgtgccgacc aaaaatccca agaaaaaacc aacccttacg
    21301 gttggttttt ttatatctgc aattaattcg ataaacagac cgtgacacat cacagcctgt
    21361 ttattttctg ttatcagaac gtccagacca cacccgcctg agtattccac ggcgattcta
    21421 cccctgcgcc attgccatag ctgactgaca aatgaccgct taacgtcggg gtaaacgatg
    21481 accttatccc agcctgataa acgccactgg ttccagccac atcattatta aatttgccat
    21541 cgtcattaac tttcacctgg ttagaatcgg cgtattcctg acgcacggcg gctttcagcc
    21601 agggttccag cgtcgtaccg ttttgcaggt ccatgtgata gcttaccgcc gttcccgctt
    21661 cagcgcgtaa tatccgggta tttcccacat ccgcgcgcat gccgtttgat aacgtgtagt
    21721 cctgaccatc tgtggtaaag ccggtaaagg ccagataggg tctaacactc cacaatccgt
    21781 caacccaacg gaacccgctc tcaacatgag cacccgcgcc gttactattg taatcgccaa
    21841 acgctgttgc cccattactc atcttgccat ggatggtgtt ggcaaaacgg tcaactttca
    21901 ccaccccatc aacataggca ccgttctgat gctcccaacc ggcataagcc cccagggtat
    21961 agctatcgat attacctttt ccgccgcgat caaaaccaat atcagaatga gagtaaccaa
    22021 agaacaagcc gcgaattgta ctgctttctt cacgggagaa acggctatcg ataccgagcg
    22081 tcaggcccgt caatgtttgc tcaaaaccag ctcccgcatc agtggtcacg ttgttgcggg
    22141 tgttaattgc cgaactccac atcaccgtat cgtaactaac gccttttacg ctaccaagac
    22201 gctcacgcac ggtgtccagt tctgcatcaa ataccagcgg ttgtgcgact gccatgttca
    22261 gcacatcagt ggttgaaggg gtaatttgcg cgcgattctc tgcgaggctc cagctatggt
    22321 tgccattatc cagcaaggta tattcatacg taccgatatc aacaacgcct ccggcattgc
    22381 ccaacgtaaa tgcagcatca ccgccgcccg ttgttaccag tgtaaggcta tctcctgctg
    22441 ccgggctggc accggtgtcc gtcacgaata ttttgaaatc acctgttgcc tgaccggtga
    22501 cgttgagctg atcgctctga tgattggcca tatcggtacg catataaaaa ttgccgtttc
    22561 ccgacagggt attggtggtc aacgtaatga aattttccgc acttgccgtc acagatgaac
    22621 gagtcactgg ctcagccatc ataatcacat ttgcgccgga catcgcaagg ttggctaagt
    22681 tgaggcgagt gttttggttc gtcgtagtga tatcggcaat gatggtccat gcgctatttt
    22741 cgagcgaaac gtccgctttg ctggtatcca cggcaccatg cgttctaaaa gaagcgccct
    22801 cagaaaccac taccttaccc accaacatag cgggtgcata atttatttgc ggcttcatga
    22861 cctcaagaat gccatcattc cctctgactg acacgttaac cattgtgcca gcccagacat
    22921 tagcgcggcc attgttgatt accatatttt cagagatggc ttttgaggaa tacgtgatac
    22981 ttccattctg gtctgatcga ccgagatcat ataccgcatt tgcctgcact ttcgtaccag
    23041 tatcctttcc cagcgattgc atagtggcat gctcatcaag gatagtgtca atggcctgcg
    23101 tgtcctccat aacaatgagc ccggaaccat catccagttc ataatttttt gacacaccat
    23161 cctttatact aaattggcct ttactgtttg taccactcac ttccagcgca ttcgttgaga
    23221 caattaattt cccgcctgct tttttatcaa cgccagttgc agtcccgcca tccataacct
    23281 ccagtaagcc gccactatcc acagtagtat tataagcgaa gtcattttct tcaactcgca
    23341 aacttccgcc gttttccaac aacatattat tagcaatacc attcttgata tcgaactgac
    23401 cgagacggtt tgttccgaat acctccatgg cccgcgtggt tgttttaata gcaccgcctg
    23461 ttttctgatc taccgcaagc gcaaatcccc cctctttaac ctccagtagt cctccctttt
    23521 cgatagcggc aaaaacggcc cccccatctt tttcaacgat taagcgcccg cctttaatcg
    23581 tggtatcatt cgctgggcca taaacctgta gcacaccatt attgagggta gtctggtttg
    23641 ccgcgccacc catttgaaca atttgataac catcatttaa tactgagtat tctgcggtac
    23701 cattcatttc aatgtattga taccctccgt taatttcagt attactactt acaccaaatt
    23761 cttttatatg ctgttctccg ccaggattga ttttcgtatt attggcggtc ccataaacca
    23821 gctggatatc tttttccagg ataacaccat caactgtctc accatactca actgttgatg
    23881 ccaatgctat gggtgaatat aacgctgtag ccaccagaac agatattgcg gaacgacaaa
    23941 gagaaacaga accagattga tgcattgagc tttcatccta tgaaattaat tgcagttaaa
    24001 agcattgggt acagaaaata cccatagctc catacccgga gtcagttttt aaaaactgtt
    24061 taaagaaatg cacaagtatt gtgattgatt ttttagttgt ttttcttgat gagaagctga
    24121 tgcaaaattc cgtctttata ataaaaatga tgccaaagcg aacgacaagg ttgtagtttt
    24181 cactacatgt ccatacataa aatggggtaa cattcacgcg cctggtagcg ttaccaacgc
    24241 tacgctcaaa cataatgatt ctaataaaac ctcaggagac tactatgcct gaagcaacgc
    24301 cttttcaggt gatgattgtg gatgatcatc cacttatgcg acgcggtgtt cgtcagttac
    24361 tggagcttga tcctggcttt gaagtggtcg ccgaagcggg cgaaggcgcg agcgcgatcg
    24421 atctggcgaa tagactggat atcgacgtga tcttgctgga tctcaatatg aaaggtatga
    24481 gtggcctgga taccctcaat gccttgcgca gggatggcgt taccgcgcaa attattatcc
    24541 tgaccgtatc cgatgcctcc agcgatgtct ttgcgctgat agacgcaggc gcagacggtt
    24601 atctgttgaa agacagcgac ccggaagtat tgctggaagc gattcgtacc ggagcgaaag
    24661 gcagcaaagt ctttagcgaa cgcgtcaatc agtacttacg tgaacgtgaa atgtttggcg
    24721 cggaagaaga tcccttcagc gtgctgacgg agcgcgagct ggatgttctg cacgagctgg
    24781 cacaggggct gtcaaataaa cagattgcat cggtgttgaa tatttccgag cagacagtaa
    24841 aagtacatat tcgcaatctg ctgcgtaaac tcaatgtccg ctcacgcgtg gcggccacca
    24901 ttctgttcct gcaacaacgc ggggcacaat aaaaatagcc cgatggattt atcatcgggc
    24961 tgagatttat gacaaacgca aaactgcctg atgcgctacg cttatcaggc ctacgtggat
    25021 cgatcaattt attgaattta cacaattttg taggccggat aaggcgttca cgccgcatcc
    25081 ggcataaact aagcgcactt tgtcatcagt ctagcccgat ggcatcacca tcgggcctct
    25141 ttttatttac tctcctgcgg cgacaaatgt tgcatcgcct gcgcgatact acgttcaatc
    25201 accgcacggc gagtatcgtt ggcaggtaag agtttcaaca tcatctccca cgcggcaacg
    25261 gcttcgccaa atcgctgctg ctcaaaggca ttaaacgcat acatgcttag cacacggata
    25321 ttgctatggt ccgttctcac cagctgacgt agcagttcac cgccgaggcg gttgtcgttg
    25381 ggatcagatg aacgagtcaa cgcttcagcg tatcccagtg cggcatcgct gttcttcgga
    25441 tcgaggcgat acgcagtagc gtatgcatcg gtggcgatac tggcgttacc cagcgccatg
    25501 ccaacgcggc ccaacataat ccagccttct atatctcccg gatttttttg cagttgagta
    25561 cgcatcccca gcgcaaggcg cgacatctct tcttcgttga gcggatcggc tttcgggtcc
    25621 agcgccctgt ccagcaacgc cggagcctgt gccgtggcct gctgccagat tttcacctgc
    25681 tgataattgc cagtctggta gtagctgacg ccagccacaa ttaacgccac cacaataccc
    25741 ggcagataaa caacatatcc ggcacgctta ccttccggca cgctttgttc aggaaacgct
    25801 tccggcacca cgcgtacccg acgccgcgag cgggcgtata tgacccaacc gccaatgcca
    25861 atagccacta ctggcagcac ccacagcagc acggtcagcg gcgttaacgg cggatcgtaa
    25921 gtgacgaagt tgccgtaacg cgccaccata taatcgacaa tctctttttt acttttacct
    25981 tcctgcatca gttcatacac tttctgacgc aggtcggtgg caatcatcga gttggaatcg
    26041 gcaatgctgt tgttctggca tttcgggcag cgcagttctt cagtgagctg acggaactgt
    26101 tgttcctgtg cttcatcttt aaactgcaac acgtcgatgg tcgccagcgc tgagccggag
    26161 atcatcagca tcagcacgcc caataaaaac ctcattgtgc ggcctcctta ctgtatttct
    26221 cccacagcgg cttgatctct tcttcccaga cgcgaggatt gagatcgccc gcatggcgat
    26281 agcgaatgat accgttgccg tcaataagga aggtttctgg cgcgccatag acaccgagat
    26341 ccagccctaa catgccgtcg ccatcaaaca ggcttagcgc gtaaggattg cccagctctt
    26401 tcagccagct gattgccttc tggcgatcgt ctttatagtt catgccgacc acgcgaatgc
    26461 cctgcgctga aagctgattc agatattgat gttccgcacg gcaggtcgga caccaagtcg
    26521 cccagacgtt aagcagtact ggtttgccct gagtcagcac atccgcctga taaaactgcc
    26581 ccggattgtc cagtgattcg agacgaaact tcggcacagg cttgccaatg agcgccgatt
    26641 ccagattggt cggatcatcc ccttcggcat tacgcgccag ctgccacagc agcgccgcgg
    26701 caatcgccag gaagataatc aacggaatta acaatacttt gcgcttcata cggcctccgg
    26761 cgcagttttt tgcggactca cgcgcttacg atagcgagga tcaaacagac acagcagtcc
    26821 gcccaacgcc atcatcagcc cgcccgccca aatccagcga acaaatggtt tgtagtaaag
    26881 acgcacggcc cacgcgccgt tttccagctc ttcaccgagc gcggcgtaca ggtcacgcgt
    26941 gatgccgccg tcaattgccg cttcggtcat catcgacccg gcagtgttgt aataacgttt
    27001 ttccgcatac agcaccgttt ccggtttacc ctctcgcgtt acgccaatag tcgccacacc
    27061 gccgcgccag ttcgggccag tcacctcttt gacatcacgg aaggtgaagc gatattcatg
    27121 aatatcgacg ctatcgccgg acttcatgcg cacatcacgc tcaacgctat aattctggct
    27181 aaaggcaatg ccaacaattg tcactgccag ccccaggtga gccgccacca tcccccaata
    27241 actgaaggtg gttttcgtgc cgcgtgaaat acgcagcgca gcttccgcag ttgccagcac
    27301 cgcaatccag caggccattg ccaggccgag caccgtcatc gccacaactt tgctttcgaa
    27361 cagccacggc aacagcagag acagcaccag cgtagagatg aaggcgataa tcaataaatt
    27421 gcggatttta cgtgggcgat cgcgccccca gcgcaccaga ggaccgacac caagcagtag
    27481 cgcaaacggc accatcagcc aggtaaacat ggtgttgaag aacggttcgc caatcgaaat
    27541 actgcccagt ccaagttgct tgtgcaccag cggcagcagc gtccccagca acaccaccag
    27601 catcgcggcg accagcaaaa cgttgttcgc taacagcaaa gattcccgcg accacagcgc
    27661 attgtttacg cgtgagcgaa ctttgtgtcc acgcgcggca aacagcagca gcgaaccgcc
    27721 aatcaccagc accataaagg cgaggataaa cataccgcgc gccggatcag acgcaaacgc
    27781 gtgtaccgat accagcacgc cggaacgcac gaggaaagtc cccaacagac acaacgagaa
    27841 ggcactgatt gccagcagta atgtccacgc tttgaagctg gcgcgttgtt cagtgaccgc
    27901 cagtgagtgc atcagtgcag tccccaccag ccacggcata aacgaggcgt tttctaccgg
    27961 atcccagaac caccagccac cccagccgag ttcgtaatag gcccatgcgg aaccgagcac
    28021 aatgccgagc gttaggaaaa tccacgccgc cagcgtccac gggcgagtaa aacgcgcata
    28081 agtgctgtcc agacgcccgc tcagcaaaga agcaatggca aaagcaaacg ccaccgagaa
    28141 acccacgtac cccatataaa gcagaggcgg atggaagatc agccccggat cctgtaacag
    28201 cgggttaaga tcgcgccctt caatcgggaa gttcggcagc gtgcgtgaga acgggttaga
    28261 ggtaaagaga atgaacagca aaaagccgac gctgaccatc cccattatcg ccagtacacg
    28321 ggccacaata tccagcggaa tacgctgact aaaaatcgcc accgcaaagg tccagccgct
    28381 catcagcagc acccacagca gaagcgaacc ttcatgcgcg ccccaagtag ccgccacgcg
    28441 ataccacacc ggaagctggg tattggagtt gctggcaaca taggtgacgg taaagtcgtt
    28501 gaccacgaaa gcattgacca gcaccagaaa tgcgccagcc acagacataa acagcagcca
    28561 ggcaaacaag cgggaagacg ccatcatgcg cgcatctccg cgcgccacgc cccatagcgg
    28621 atacacggac agcagcagcg caatccctaa cgccaagcac agcagcccgt ttccaatttc
    28681 cggcatcatg atgctgggtc cttataaaca ctcgccgggc gacggtggtt agcttccatc
    28741 gctttctcaa cttctggcgg cgtgtagttt tcgtcgtgtt tcgccagcac ttctttcgcg
    28801 aggatatgat tgcctttttc cagctcgccc tgcaccacaa cgccctgccc ttcacggaac
    28861 agatccggca aaatgccttc gtaagagaca tccactgagc cttcagcatc gtaaatggtg
    28921 aaggtcactt tcagcgaatt gggatcgcgc tgcacgctac ccggcatcac catcccgcca
    28981 acgcgcagac gctgaccgac ttccggcatc tgctgagttt cacgcttgcc gtagagaatt
    29041 tcccccggcg tataaaagag atcgatattc gagcgcagcg catatagcac cagaccgata
    29101 gtcagcgcta gccctgccaa cacggcacag gcaatccaca agcggttttt acggcgaata
    29161 ttcatgcagc ctcctgctgt tgcgcagcac gtaaacgcgc ctcacgcgcc cgctgttgcg
    29221 ccacaccacg cagaattgcg cgatgttgca tcaccgagtg cacgaccaaa accaccagcg
    29281 gaataacggt catcaccacc gccagccaga caaaaaaggc gtaaccgccc attgcgaaaa
    29341 attcattcca ggaagcaaat gcaggggtca tttacggcct cttttcagta tcagttcact
    29401 cacccacgga cggcgttttt ccatcagcaa aatcaaatta cgcatccgca tcagcgtcag
    29461 cgtggcagac aggagcagga agccaaaaat cgaccagcgc agcggcgaac gcatcgccgg
    29521 atcgatactt tgctgcatcc gcgttgatcc ctgatgcagg gtgttccacc actccacgga
    29581 gtaatgaata atcggcagat tcaccacgcc aatcagcacc atgatacctg ccgcacggcc
    29641 cgccagacgg cggtcgtcga aggcgtgcca cagggcaatc acacccacat acaaaaacag
    29701 cagcaccagt tcagaagtca gacgtgcatc ccatacccac caggtgcccc acatcggttt
    29761 tccccatgca gagccggtaa ccagggcaat aaaggtaaac acggcaccaa tgggggccat
    29821 cgccgccacc gccaggttgg ccattttcat ctgccagaca aggccaataa acgccgccac
    29881 cgccattgat gcataaatgc ccatcgacca gatcgccgca ggcacatgca ggtagataat
    29941 gcggtagcta tttccctgct gataatcagc cggagcaaag ccgaatcccc agatccagcc
    30001 gacggtaagc acgaccacac tggcaattgc cagccacggt ataaaccagc cacagatttg
    30061 atacagccgt ggtgggatcg ccagttgatg cagtgttttc cacatagttt cgataccaga
    30121 ctcgaacaaa aatcagtaat ccagcgttat tgaatgctga ttcgtaacgc tgccgccgtc
    30181 gcaaaaggac ttaatgtcgc ggtgcctgcc agcaacgcgc ctaaaattgc cagataccca
    30241 tcaacgggca aatgcataga agccgcgtcc atcgcggcgg tggcaaagat gagtaatggg
    30301 atagtcagcg gtaacaccag tatgctgagc agcacaccac cgcgcttaag tcccactgtc
    30361 agcgccacgc ccggtgcacc gagaaagcca agcgtaggcg ttcccagcag cagcgtcagc
    30421 gccatcactt gccagccata aacatccatt cccagtagca ttgctaccag tggcgaaagg
    30481 atgagtaacg gcagaccggt taccatccag tgcgccatca ccttcgccag cacaacggcg
    30541 ggcaagggta acggcaacaa catcaattgt tcaagactgc cgtcctgcaa atcgtcacgg
    30601 aacagtcgtt ccagcgccag caaggatgaa agcagcgcag caacccagat aatgcccggt
    30661 gcaatacgcg ccagcagttg cggctccgga ccgatactga gcggaaaaag ggtaattaca
    30721 atcaggaaga accacagcgg gttggcgatt tcggcgctat ggcgaaacgc tacgcgcagt
    30781 tcaagacgga aaatacgcca gaacatcatg cggccctcgt ttgcgtcagt gaaatgcggc
    30841 gaattttact ttcagcaacg ttgagcggct ggtgggtagt cagaatcaca atccccccct
    30901 gctccgtatg ctgcgccata cgctgggtca gacgatcgac gccgttaacg tcaatcgcgg
    30961 taaaaggctc gtcgaggatc cataatgtgg cacgggtcaa ccacagacgc gctaaagcga
    31021 cgcggcgttg ttgcccggca gaaagttgat ttaccggaat atcttcgaat ccggcaagcc
    31081 cggcctgcgc caaggcttcc agacattgtg cggcatcgcc atcgcgatga taaaagtgca
    31141 gattttctaa cgccgtcagc cgggttttga tccccggctg atggcctatc cataacaggg
    31201 tttgatggta gctgtcgcgt acctgatgca agggctgccc ttgccagaga acctcgccag
    31261 cgtcagggcg agacaacccc gtcagcaaac ggagaagcgt tgtcttcccc gcgccgttgc
    31321 taccggtgat ttgtacccac tctcctgcgt tcagcgtaaa tgacaagcca ctaaataagg
    31381 ttcgttcatc ccgctcacaa agtaactctc tggcttcaag catacccacg caataaccct
    31441 gttaaaaacc tggctcgact tcacgcatat cgggcagctt gtgcgcgatc cctttatggc
    31501 aatcaataca ggtttgccca tctttcaccg cctggtcatg catcttcgcg gcaaccgatt
    31561 tctgggcggt tgtatccata tactcgaagt tgtgacagtt acggcactcc tgcgagttat
    31621 tgtccttcat gcgccgccac tcattctgtg ccatcgtcag acgatgagct tcaaatttct
    31681 gcggcgtgtc aataacgcca aaaattttac catacagctc tttacttgct ttgagcttgc
    31741 gtatcatctt cggcacaaac tcgtgcggaa cgtgacaatc cggacaggtc gcacggacgc
    31801 cgctacggtt gttgtagtgc acggaatcca tgtattcctg atacaccgtg ttgcgcattt
    31861 cgtggcagct aatgcagaac tcttcggtat tggctttttc catcccggtg ttaaagcccc
    31921 cccagaagac gatgccgcca acaaaaccga tcaacagcag cgtccccagc gccagatggc
    31981 tgggggtacg ccaccatttc cacaggcgct taatcagacc aggcttacgg tcagaatttc
    32041 ccataataac ctcttatttc ccgtaacctt ttgatggggt aaaggtattc cccacgattg
    32101 gcgcggtatc ggcctgcggt acgtgacatt gcagacagaa ataacgacgc ggagccactt
    32161 ccgcgcccac tttgccgtcg ctgtccataa agtgagtagg actgatacgc ggcgcgccag
    32221 tggtgcgata gctttcgaca ccgtggcact gcaagcagcg attggtattg gtcgttacct
    32281 gataaccttc aacgctatgc gggatcattg gcggctgatt cacatagttc agcggcatcc
    32341 gatcctgctc ttttggcatc cgaatggccc cttcctgcgt cccggatact tccggcgact
    32401 ggctaaaatc gacgccatta gccgcccaaa ccgcgccgct taccaccagg gccagcatcg
    32461 ccgtccattg acacagcgct ttcttcaggt catggctttt catgatttcg ctcccgaact
    32521 ccatcgtgta gttattgtaa atacatcctc agaacagaca tccacgcagc gaccgcacgt
    32581 catgcaatcg cggctggtga cctgcaccgg gctttgttca tccagcaccg gggcacgtag
    32641 cacatgcggt tccgggcaaa catgaaaaca atccatacag cggttacatt tctgccgatc
    32701 gctggccgca acggtaatca cacctttgct acccagcacg ccatacagcg cgccaaccgg
    32761 acaaatgtgc ccgcaccagc cgtgttcaac gaccagtaga tcaaataaaa acagcgcgag
    32821 aataagcagc gcgccgctgc cgaagcccat taccaggcta cgccccatca gagaaacggg
    32881 gttgatccat tcccaaatga gcgtgccagt taaggctgat cccaccagaa tgaccaccaa
    32941 cagcacgtag cgaatgtggc gagggatcgt cgcagactga ttgaggtcaa agcgcctgcg
    33001 taaccagttc gctaagtcgg taatcgggtt cagcgggcag acccagctgc aaaataatcg
    33061 ctttcccgcc agggcgtaga gcacggtgat aatcaccgca ccggtcaacg ccaccgtggc
    33121 aggcagatga ccactggcaa gactttgcag cgtcatcagc ggatcggtca gcggaacggt
    33181 gtcgaataac aggctactgc tgtagttgcc gtgcaagatc cacacaccaa accatggacc
    33241 gctcaaaaac atccccagca cgaagaactg acaaaggcga cgtaacacca gccaacggtg
    33301 actgcgccac cagccttttt tctccagcgc ctcgcgcccg gcgtcacgtt tacgatttgc
    33361 cattgttccc ctccagccag ccgaagcggt aatggtgacc taactcccct ttcgccagtg
    33421 acagcggtaa caccttgatt gccggttgtt ccagcacgca tactttttcg cacttaccgc
    33481 aaccggtaca ggcgtcgctg tgaaccgtcg gcagaaagcg ggcgtgctta ccggtacgcg
    33541 tgttgcgctc cagctccaag gtgatggcct catcaatttt cgggcattcg cgataacaaa
    33601 catcgcagcg cagcccctga aagttgagac agttttcctg gtccaccagt accgccagcc
    33661 ccatccgcgc gtcgtcgatc gattcaatct cacgatccag cgcaccgctt gggcacactt
    33721 tggcgcacgg aatgtcctca cacatttcgc aaggaatatc ccgtgcgaca aaatatggcg
    33781 tgcccgccga cagaccagag gccagcgtcg ccagttttaa ggtgtcgtaa gggcaagcct
    33841 gaacacactg accgcaacgc acacaggcac tggcaaaggc gttctcgttt atggctccgg
    33901 gcgggcgcaa ccgcacgcca gatgcgcgtg cggtttgctg ttgtaacccc agcgccacac
    33961 ccacggcagc cagcccgcct gctgtgcgaa cgacatcgcg cagaaagcgg cggcgaccat
    34021 tttgaggttt cgctgaccgg gacataatgg cttacacctt ctccagtttg accgcgcact
    34081 tcttgaagtc cgtctctttc gagagcggat cggtcgcatc cagcgtcagt ttgttaacca
    34141 gctgtgcggc gtcgaagaac ggcatgtaca ccaggccctg tggcggacgg ttacgaccgc
    34201 gcgtttcaac aatcgagatc acttcgccac ggcgagaaac cactttcact ttgtcgccac
    34261 ggcgcagatc gcgcgctttc gcatccagcg ggtgaataaa caagaccgct tccgggaagg
    34321 cgcggtgcag ttccggcaca cggcgagtca tactgccggt gtgccagtgc tccagaacgc
    34381 gtccggtaga gagccacagg tcgtactctt catccggtgc ttccgccgcc ggttcgaacg
    34441 gcagtgcgaa gatcaccgct ttgccatccg gtttaccgta gaacttatag ccttcgcccg
    34501 ctttcacgta cgggtcgtta ccttcgctgt aacgccactg tgtttcttta ccattcacca
    34561 ccggccagcg cagaccgcgc gctttgtggt agtcatcgaa cggtgccaga tcgtgaccgt
    34621 gaccgcgacc aaaccatgcg tactcttcga acagcccttt ttgcagatag aagcccagct
    34681 cgcgggattc atcgttcagc tgatcttccg ccagttcgga taccgggaat ttgctcactt
    34741 cgggggtggc atacagaact tcgtacagcg ttttgccacg cagttccggt ttcttcgcca
    34801 gcagctcttc cggccatact tcttcagttt tgaagcggcg ggagaactgg actaactgcc
    34861 agagatccga tttcgcttcg cctggcgcct gtacctgctg acgccagaac tgagtacggc
    34921 gttcggcgtt accgtaagcg ccctctttct ctacccacat tgcggtcggc aggatcaagt
    34981 cggcggccag cgcactgact gtcggatacg gatcggagac gatgatgaag ttgcgcggat
    35041 cgcgccagcc cggcatacgc tcttcattga tgttcggccc ggcctgcatg ttgttggtac
    35101 acatggtcca gtaaacattc agcttgccgt ctttcagcgc acggtcttgt gccaccgcat
    35161 gaagaccgat tttcgccgga atggtgccgc tcgggatatt ccacttcttc tcacagatat
    35221 cacgatgttt ctcgttagtt accaccatgt ccgcaggcag acggtgagca aaggtgccca
    35281 cttcacgcgc agtaccacac gcagaaggtt gcccggtcag ggagaacgga ccgcaacccg
    35341 gctgggaaat tttgccggtc agcaggtgca ggttgtagac caggttgtta gcccacacac
    35401 cacgggtatg ctggttgaag cccatcgtcc agtaggagat gactttcttg ttcggatcgg
    35461 catacagctg cgccagttgt tctaactggt ctttcggcac gccggtcatt tcagcggttt
    35521 tttccagcgt atactcagca acgaaggctt tgtaatcttc aaagctcatc ggttcggagg
    35581 cgtcagaacc cggattcttc gctgcttttt ccagcggatg ggtcggacgt aaaccgtagc
    35641 cgatgtccgt cgcccctttg cgcaggttaa cgtgcttgct gaagaagtcc tgatttatcg
    35701 cattgttttg aatgatatag ttggcgatgt agttcaggat caccaggtca gattgcggcg
    35761 taaagatgat gccgttatcc gccagctcga agctacgatg ctggtaggta gaaagcaccg
    35821 ccacggtgac gttctggtta gagagacgac tgttagtgat gcgtgaccag aggatcgggt
    35881 gcatctccgc catgttagcg ccccacagca caaacgcgtc agcctgctcg atgtcgtcat
    35941 agcagcccat cggctcatcc ataccaaagg tacgcataaa gccaactact gccgacgcca
    36001 tacagtgacg tgcgttcggg tcgatgttgt tcgaacggaa gcccgctttg aacagcttgg
    36061 acgcggcata accttcccag atagtccact gaccagaacc gaacataccg atcgattccg
    36121 gccctttttc tttcagggcg gttttgaatt tctcttccat cacatcgaag gcctgatccc
    36181 aggtgattgg ggtaaattcg ccttctttgt catatttacc gtttttcata cgcagcagcg
    36241 gctgcgtcaa acggtcttta ccgtacatga tcttgggcag gaaatagccc ttaatgcagt
    36301 tcaggccacg gttaaccggt gcgtccgggt cgccctgaca ggccaccaca cgcccctgct
    36361 gcgttccgac cagaacgccg caaccagtac cgcagaaacg gcacggcgct ttatcccatt
    36421 taatggcttc ctgctgacca acaacggcgc gggcaacgcc cggcacgctg agaccggcag
    36481 ccgccgcagc ggccgcaacg gcgttagctt tcataaagct acgacgactg agtttcatgg
    36541 tgtttcctca ccttgctctt cctgctggtg ataaaccagc gacaccgcca gcacgccctc
    36601 tacgttgcgt actgactcaa tggtttggat cagcgtttcg ctgtcttctg cttccaccac
    36661 cacaatcaac tgaccgctcg gcgcgtcgct gacagcaact tcacagccgg gaaaggcgtt
    36721 cagttgggtg ctgatgtctg aaattcgttc gcttttggcc tgcacgacca ggctgcaaac
    36781 ttgccagtta gtgtgcatgg agatactccg cagttatggc tgataccgga cagctggctg
    36841 cacatgctcc acatccgttg caaagttggc tattaagttg cgcctggtag atcccggaga
    36901 gcgtcgggcg aaagataatt gccattggct cacagctatc ctgacagcgg cggcattcaa
    36961 ctgactgata cgccaggcac gcgtccccga tggtgaactg caaatcccag gccctggtgt
    37021 ggcgcggaga aaatagcgat tcggggcaag cctgcgcgca ggcgtaacag aagctgcact
    37081 cgttattttt gaaattaacg ctcggatagc cacccgtgcc gcgttgcaga atgttgtttt
    37141 cacaagcatt aatacaggcg tcgcagcggg tgcaatgggt cagaaaatga gattcatcac
    37201 cggaccaggg cggacggata ccgttactgg ctttgcgcca gcaaccagtg agtatgcccc
    37261 gacgggatgc atcaatcttc acattgacct tccatcatta acgcgctctg aaaattgaga
    37321 gcgaccaaat agaccgcata attaataagc catttttata gccgctaaga tattaaagga
    37381 tgtgtcaaag atgcataccc cgatcggggt aaaacctgta gcaggatcaa aagagtggcg
    37441 ggaagcgtgg caaaaacggg cttttgctca catttcaaat ggttataaac atatttatat
    37501 agcgattaat tcaccagaga tatttctgct ggtttgctcc ctcatcagaa tttaacacta
    37561 aaagagcagg taaaattgtc tgaatgttct ttaagttatt cataaagcaa attaataaat
    37621 ctgatgaata tgttaacctt cagcgacatc atcggtgaaa acctataaat gaagaaggaa
    37681 agcaaaaaaa tgaagaccat tctacctgaa gtattgtttg ccgctttcgc taccacttcc
    37741 gcctgggcgg cagaaagcgt ccagccactg gaaaaaatcg cgccttatcc acaagctgaa
    37801 acaggcatga agcgtcaggt gattcagtta accccgcaag aagatgaatc taccctgaaa
    37861 gtagaactgt taatcggtca gacgctggaa gtcgattgca atttgcatcg tctcggcggg
    37921 aagctggaaa gcaaaacgct ggaaggctgg ggctatgatt attatgtctt tgataaagtt
    37981 agctcgccgg tttccacgat gatggcctgc ccggatggca agaaagagaa gaaatttgtc
    38041 accgcgtatc tgggcgatgc tggaatgctg cgttacaaca gcaagctgcc gatcgtggtg
    38101 tatacgccag acaatgtaga tgtgaaatac cgcgtctgga aggcggaaga gaaaattgac
    38161 aacgcggaag ttcgctaaac tgttgtgaag tacggcaccc cgttggttgg ataaggcggt
    38221 tacgccgcat ccaacatcta acgcccgagc cggttgcctg atgcgacgct ggcgcgtctt
    38281 atcagtatac gccacatcat tacaacgcaa tatccgccac ttctttttga acgggttgcg
    38341 gtttcaactg cggtttcggc gtactatctg ctgcttgcgg tttgtcgtat ttcagcccca
    38401 gcacttcgct ggtgtactgc aactcgcgtt ctgttgccgc tacatcaccg ttcagcttgc
    38461 gtccataaga cggaacgatc gctttcaacg tagcctgcca ttgcgggctg gaaacgcgat
    38521 cgccaaatac tttttccagt agattcaaca taatcggcgc ggcggttgac gcccctggcg
    38581 atgcccccag cagcgccgca atggtccctt gctggtcact gaccacttcg gtacccagac
    38641 gcagtacgcc acctttctcg gcatcacgct tgataatctg cacacgctgc cccgcttgcc
    38701 acaaacgcca gtcctctttt ttcgcttgcg gatagtactc tttcaacgct tcaaaacgat
    38761 cctcttcact caacatcacc tgactcacca gatatttcac cagatcgaaa ttatccagcc
    38821 cgacgtgcat catcggcatc acgttagagg tggtggtgga actcatcaga tcccacaatg
    38881 aaccgttttt gaggaatttg gttgagaagg tggcaaatgg cccaaacagc actacgcgtt
    38941 taccgtccag aacacgggta tcgatatgcg gaaccgacat cggtggtgcg ccaacggatg
    39001 ctttaccgta aactttcgcc agatggcgat taaccacgtc tggattttcc gaaacaagga
    39061 actgtccgcc caccgggaaa ccggcgtagt ctttcgcttc cggaatcccc gattcctgta
    39121 acagcttcag cgccgcaccg cccgcgccga taaagacaaa tttggcgcgg atgttctgtg
    39181 cagtgccgtt tttcagatcg gcaacggtaa cggtccaggt gttatcgtca ttacgcttta
    39241 gggcgcggac ttcgctgctg agttgcagcg agaagttaga tttcttctgt aaggaagcaa
    39301 ttaactggcg ggtgatctca ccatagttca catcagtgcc aatttctgtt gcggatcgcg
    39361 cccttccatc actaacggtg cccactcttt gatctgcgcg tgatcttcag agtaacgcat
    39421 accgcgaaac agcgagcttt gttgcaacgc ggcgtaacgg gcgcgcagga aattaacgtt
    39481 atcctcgccc cagacaaagc tcatatgcgg aacggtattg ataaatgaac gcggagtacg
    39541 cagcacgccg cgctcgacct ggtgcgccca gaactggcgg gaaatctgaa atgcttcgtt
    39601 aatggcgact gctttttcaa tactgatgct gccatcggcg ttttgcgggg tgtagttcag
    39661 ttccatcagt gcagaatgcc cggttccggc gttgttctag ccgttcgaac tctcctgcgc
    39721 gcccccctcc aggcgctcca ccatggtcat cgaccattca ggctccagct cgcgtcaata
    39781 ggtccccaac gtggcgctca taatgccgcc gccaatcaac agtacatcag tttcctgctc
    39841 ctcggacgct ttcgcttttg ccgccatcga aacggcatta agccccacgg ccatcgagaa
    39901 gagcatggca gtcacttttt tcatcttgtt aatgccttac ttttagtcgc tttattgcag
    39961 gtgagatttg cgcggcatca acggtaacac ttaagtaaca acatttaaat aatgtttata
    40021 aattatattc taattttaga aacattataa tttcgttgat taattatagg gttattagaa
    40081 atgaagggat ttttagcata ttgacctgac ggcagcagtc cgccaggtca gcgtcgtaat
    40141 attatgccgt ccgggcaacg gcatcacgcg aagcggcatc gcgctcttcg cccgtcagct
    40201 cgctaagttg cccattgcgc atttccagca ggcggtcggc gtggataaag taatgatcat
    40261 catgactgat agcgaaaata gttttaccca tctcctgcat cagcggcagc aacacctgat
    40321 aaaactcacg acggaagtgt ggatcctgat ccaccgccca ttcatccagc aggataatat
    40381 cgcgttcttc tgccagcgcc agcaacagcg ccacgcgttt tttctgccct tttgataact
    40441 tcaggttaac aatacgcccg ttgcttaact caagcttatg agccattttc agctgcgcca
    40501 gccacttctc aaccagttgc gggttagcgg gttgcccctc cggccccagc agttgatcaa
    40561 acagccagac atcggtaaac actgccgaaa acagtttgcg ataatcttcc ggttgttcgc
    40621 cgctgacagg tttgccatcc agcaagattt cgccgctttg tggctgatac aagcccgtca
    40681 acaacatcgc cagcgtcgat tttccgctac cgttaccgcc aatcagaaac agcagctcgc
    40741 cacgtttgat ggtgagatta atcggaccaa cggaaaacgc gttatcctga taagcaaacg
    40801 tcacgttacg cagctccagc gtttgccagt tgggaaacgc ctgcgggcgc ggaaactcag
    40861 ctttgaaagg cgcgagcgcg aatttgttca gcttgttaaa cgccacctgc gccgtcagca
    40921 gcgtcggcaa tgcgccaacc gccgaaagca gcggcgtacg caggaataac agcgtcaacg
    40981 aataggtcgc agcaacgttg gtatcagccc aaccgagact gttcgccatc cagaacacca
    41041 ggccgatagc gcccagcatc atgatgtttg accagttcac ggcactaaga tggaaggtgt
    41101 ctgcgcgaat aatatgatgg cgatactctt gcgcatcggg gatgtagagg ttgttaaaca
    41161 catactcggc gcgttcccgg ttcagagtca gctctttgcg cccttcaagt acagtttgaa
    41221 aatccgtgta cagcttgtct tcggtttcac gtagggtcgc catatgtttg tacacccgcg
    41281 ccaccagcac aaaaccgccc cagatggtga tcgccatcca gatagccgtt accagcaaca
    41341 ttttgcccga cagcatccac agatacgccg atgaaccgat agtgagaatg atcccctgcc
    41401 ccagttccgg cagacgcaca aaagcaatgg tgatattgcg cacatcgctg gttaatcccg
    41461 ccagcaacga ggcgctaccg agttgttcaa tgcgctcgac gtgggtatcc agaatccgct
    41521 tgataaattc actacgcagt cggtaaacga agtgatgccc caaagtggtg agcgccagtt
    41581 gcgatccgag agtgactgcc atcaacagca gcaataatcc cagaaactcc ggtaacacca
    41641 gcagactggt atccgccgtt tcgataaggc gctgattgat aaaagcaatt aagccaatgc
    41701 ctaatgccgc actggcgagg cttagcgcca tcacactgat aaatggccag cgatactgcc
    41761 gccagacaag tacaagaagt tccatgcaga aaacccggac aatgaattac agcccgcagt
    41821 ttaaacatct tcgcgcgcac agcaataata attcttattt ttattctttt ttacctgcct
    41881 gacggaatgt caggttgtag cggcagtcgg tggtgagtgg atgaaacccc gctttcaacg
    41941 gttgaatacc gtgataaaac agccgcgatt caccgcccca taccaccaca tcgccatgtt
    42001 ccaacaacaa acgtttgagc ggatcattac gtttcaggcc accaaattga aaaatcgcgg
    42061 gtaagcccag agaaacagaa acaattggcg cgcgcagatc cggttcgtct ttatcctgat
    42121 gcagcgacag tttcgcgcca ggagcgtagc ggttgataag acaagcatct ggctggaaat
    42181 ctggatagcc cgccgccgta gccgcacgtt gacataaatc atgaaagctt tgtggcatag
    42241 cgggccacgg tttatttgtt tgcggatcaa tgggcgaata gagataacct tgccgatggg
    42301 tcgtccagcc cagatgccca cagttggtca tcgccaccga catggtatat ccgcctggag
    42361 cgaccatctg gcgaaacggc gactggctgg caacgtcatt aatatcgcgg attagttgct
    42421 ccgcagcatt aaaagcaaaa cgccgtaaaa ttaccgcacc agccgccagt ggctcttgcc
    42481 acggttcagc atcggcaaac agatccaaca ttacctctcc tcattttcag cttcgcggcg
    42541 cagcagttgc gctttacgcg acacgcccca gcggtaaccg gaaagtgtgc catcaccacg
    42601 gaccacccga tgacagggta ttacgatagc cagcttgttg gcggcacagg cgctggcaac
    42661 ggcccgtacc gctttcggtt tgccgatggc gttagccagt tgctgataac tgacggtttc
    42721 accacaaggt atcgtgcgca gtgcctgcca gacttgttgc tgaaaagcag tgccgcgaat
    42781 atccagcggt aacgtcagcg gcgtatcgcg ttgattgagg ctggcgatca cttcacgcac
    42841 atgttgctga aacgtcagat cggcaggcgc gttgtcggca gcgggaaaca tctgctgcaa
    42901 ctcgctgatt aatgtcgcgt catcatcgcc cagcaatatc gcgcaaatcc cccgctcgct
    42961 ttctgccacc aggcaacgac ccagctcaca atcagccagc gcgtaacgca ccgccagatt
    43021 ttcgccaccg tgacggaatt gtttagccgt catgcccagc gtttcatcgg ctttgcggta
    43081 gtaactgctg ctgtcgggga atccggcgtt aagaatagac gtcgtcacgc tctccccttt
    43141 cgccagcgat tcgcgcaaac ggcgagcgcg ccaggcctgt tgccaggctt taggcgtcat
    43201 tccggtagta gctttaaaca accgatgtag atgaaatgga ctcatcgcca cctggtcggc
    43261 taaggcttcc agcgttacag gcgtttcctg ttccagcagt cgacacgcgt gggtgatttt
    43321 atccaaccga tgttgccggg gattggcttt gtctggctga caacgtttgc aggggcgaaa
    43381 tccggcggcg agtgcctcgc tggcatttgc gtagaaggag acattttccc gcaaggcatg
    43441 tctggcgcgg caagaaggac ggcaaaagat acccgtggtg cgcacggcga aaacgaattc
    43501 gccgtcggca ttcgggtcgc gggctaagac agattgccag cgttgatcgt cagttaagca
    43561 tgtggcgttt ttcataatca gctccctggt taaggatagc ctttagtctg cccggtcacc
    43621 atcacgcaaa aaccaacaat cttgcgcttt aatttttttc gctgacaagg aagcttttaa
    43681 actgtggtga catccaggtt ttaaagctat cgccttcttt ggtgatcata taaaccgcca
    43741 gcccttcccg gcgaacaacc tctttggctt tctccggccc gagtaccatc aagccagtat
    43801 cccaggcatc ggcttccagc gccgtcggag caatcaccgt cacggatacc agattgtgtt
    43861 cgatgggacg cccggtttgc ggatcgataa catgggaaag acgtttgccg tccagttcgt
    43921 aatagttacg gtagctgcca gaggtgctaa tcccatgacc gttgatatcc accacagcct
    43981 gaaccgcgtt ttctttatcg gttggttttt gaatcgctac ccgccacggc tggccttcac
    44041 cgttcatacc acggctgttc agcgcgccgc ccaccgacac cagatagcgg gaaatccctt
    44101 cctgctccat caagcgtgcc aggtgatccg ccgcataacc ttcaccgacg gtggagagat
    44161 cgacatataa atccggcagg tctttttgca gatattgctg atgcgactga ttgatgaccg
    44221 tcaggtgctg taagccggtt ttggctttca tcgcatcgat ctgttcctgg ctcggaattt
    44281 gaaccggctg ttgttccggg ccaaagcccc acagattcac cagcggcccg acggtaatat
    44341 ccatcgcacc atcggtcctt gccccaatgc gcagcgaggt ggtgacgata tcggccatcg
    44401 cttcacttac cggccacggc gacaaacttt gcgagtcgtt aaagcgcatc agcgcggaat
    44461 cttttttata ggtcgaaagc agctgatcgt cggcgtccag ctgggtctga atcttttctt
    44521 taagttcggc actgcgtttg gcatcaatgc ccgggatgct ggcacgccag aaggtaccca
    44581 tggttttgcc ttcaagaacg gtaacttcag tagcgtgggt tttggcgggt tgtggttttt
    44641 gatcgcaacc aacaaagaag agcgcggcaa ccagcagtgc cacgcgggta aagcttattt
    44701 ccatacgtga ttatcctcat gcgaacggtc gcaagagtac accaaaaaac tgtgtttgta
    44761 cgctgaaaac aatgaaaaaa gggcccgcag gccctttgtt cgatatccat cgagattaga
    44821 actggtaaac cagacccaga gctacgatgt tatcagtgtt gatgccagcg tcacgagtga
    44881 actggttgtc gtccagcagg ttgattttgt agtcaacgta ggtggacatg tttttgttga
    44941 agtagtaggt cgcaccaaca tcaacatatt tcaggatatc ttcgtcgtcg tagttacgac
    45001 cattgatgac acccaggttt ttacctttag actgcaggta agccagggac ggacgcagac
    45061 cgaagtcgaa ctggtactga gcaacagctt cgaagttctg tgctttgttc gcccaaccca
    45121 agttgcctac gcgagttgcg ttgtaggtct gggtgtactg agcagctagg tagatgttgt
    45181 tcgcgtcgta tttcagacca ccggtgtagg tttcagcgcg gtcgccatta ccgatgtaac
    45241 gctcatcata acggttcaga ccgaagttct ggtcatcagt acgtttggag ctggaaactg
    45301 cagcaccgat accgaagcct tcgtaatcat aagtaataga accgccaacg ccgtcaccgt
    45361 tctgacgcag agcttcacga ccgttgttgg tcatgccttc accttccggg ctaccgtttt
    45421 tgccctgata ctgaacagcg aagttcagac cgtcaaccag accaaagaag tcggtgctac
    45481 ggtaggttgc gaagccgtta ccacgctgct gcatgaagtt gtcagaaccg taagtgtcac
    45541 caccgaattc tggcagtacg tcggtccagg aagttacgtc gtaaactacg ccgtagttac
    45601 gaccgtagtc gaaagaacct acatcctgga atttcagacc tgcgaatgcc acacgggtcc
    45661 aggagttgtt ttcgttttca gcgctgttgc cctggatctg atattcccac tggccgtaac
    45721 cggtcagctg gtcagtaacc tgagtttcac ctttgaagcc aagacgcatg taggtctggt
    45781 cgccatctac agacttgtcg tcagagaaat agtgcaggcc gtctacttta ccgtacagat
    45841 ctaatttgtt gccgtctttg ttgtaaactt cagcagcgtt tgctgcgcct gctaccagca
    45901 gagctgggac caggagggac agtactttaa ctttcatgtt attaaccctc tgttatatgc
    45961 ctttatttgc ttttttatgc cactgcatac tgattaaccc tcattaatca gtcggcaagt
    46021 ccattctccc caaaaatgca gaataatcca acacgaatat gatactaaaa cttttaagat
    46081 gtttcattta tcgctataga tgtttcaaaa tgtaaataca agggaacttt ttaagattat
    46141 tgcggaatgg cgaaataagc acctaacatc aagcaataat aattcaaggt taaaatcaat
    46201 aacttattct taagtatttg acagcactga atgtcaaaac aaaaccttca ctcgcaacta
    46261 gaataactcc cgctatcatc attaacttta tttattaccg tcattcattt ctgaatgtct
    46321 gtttacccct atttcaaccg gatgcctcgc attcggtttt tttacccttc tttacacact
    46381 tttcattatt ctgtgctacc acagaaaaac tataacgctt gttaactatt tcacaaataa
    46441 ttaacatcag cataatttcc agcaatcttt gtttatttgc aattaatttc gttgggcttt
    46501 ttgtaggtta tttgtacagc aaaatggcgc ttgtacatct atttcccccc atacaggatg
    46561 ataaatatca cgggagaata gagaatcatc aatcaggtaa gagtctggaa tttcacactg
    46621 taccctttat actgccctat cacttcgcga agttttaaca ggtcataaac acgaatgcgt
    46681 cagaaagaga caacggccac gacccgcttt tcactcctac cggggagcat tacccgcttc
    46741 tttttactgt tgatcattgt gttactggtg acgatggggg taatggtaca aagtgccgtt
    46801 aacgcttggc tgaaagataa aagttaccag attgtcgaca ttacccacgc aatccaaaag
    46861 cgcgtcgata cctggcgtta cgtgaccagg cagatctacg acaacattgc cgcgacgacc
    46921 tccccctcct ccggcgaagg tttacaagag acgcgcctga aacaggatgt ctactatctg
    46981 gagaaaccac gccgcaaaac ggaagcgtta atctttggct ctcacgacaa ctcaacgctt
    47041 gagatgactc agcggatgtc cacttatctg gatacattgt ggggtgcaga aaatgtaccg
    47101 tggtcgatgt attacctgaa tggtcaggat aacagtctgg tgctgatctc aaccctaccc
    47161 cttaaagatc tcacctccgg atttaaagaa tcgaccgtca gtgacattgt tgattcacgt
    47221 cgtgcagaaa tgttacaaca ggccaacgcc ctcgatgaac gcgaaagttt ttctaacatg
    47281 cgccgcctag cctggcagaa cggtcattac tttaccttgc gtaccacgtt caaccagccg
    47341 ggacatctgg caacggtcgt ggcttttgat ctgccgatta atgatttgat cccaccgggt
    47401 atgccgctgg acagtttccg ccttgagcca gacgcgacgg caacgggaaa caatgataat
    47461 gagaaagaag ggacggatag cgtcagtatc cactttaaca gtacgaagat tgaaatctcc
    47521 tcggcactca actctaccga tatgcgcctg gtctggcagg ttccttatgg caccttattg
    47581 ctggatacgt tgcaaaacat tctgctgcca ctgctgctga acatcggttt gctggcgctg
    47641 gcgttatttg gctataccac attccgccat ttctccagcc gcagtacaga aagcgtcccc
    47701 agcacggcgg tcaataacga attgcgcatt ttacgggcaa tcaatgaaga gatagtctca
    47761 ctgttgccgc tcggcctgct ggttcacgat caggaatcga accgcactgt cataagtaac
    47821 aaaattgccg atcatttgct gccgcatctg aatctgcaaa acatcaccac catggcggaa
    47881 cagcatcagg ggattattca ggcgacgatc aataacgagc tgtatgagat ccgcatgttc
    47941 cgcagccagg tcgcgccgcg cacacaaatt ttcattattc gcgatcagga tcgcgaagtg
    48001 ctggtaaaca agaaactcaa gcaggcgcag cgtctgtatg agaaaaacca gcaggggcgg
    48061 atgaccttta tgaaaaacat tggcgatgcg ctgaaagaac ccgcacagtc cctggcggag
    48121 agtgcggcta aactcaacgc cccggaaagc aaacaactgg cgaatcaggc agatgtgctg
    48181 gtgcggctgg tcgaagaaat acagttagcg aacatgcttg cggatgatag ctggaaaagt
    48241 gagacggtgc tgttctccgt gcaggattta attgatgaag ttgtgccttc agtgttgcct
    48301 gccatcaagc gtaaaggtct gcaactgctg attaacaatc atctgaaagc acacgatatg
    48361 cgccgcggcg atcgcgatgc cttacgacgt attttgctgc tactgatgca atatgccgtg
    48421 acctcaacgc aattgggaaa aatcaccctt gaggttgatc aggatgagtc ctctgaagac
    48481 cgcctgacat tccgcattct ggacacggga gaaggcgtaa gtattcatga aatggataat
    48541 ttgcacttcc cgtttatcaa ccagacccaa aacgatcgtt atggaaaggc ggacccgctg
    48601 gcattctggc tgagcgatca actggcacgt aaactgggcg gtcatttaaa catcaaaacg
    48661 cgggatgggc ttggtacacg ctactctgtg catatcaaaa tgctcgccgc tgacccggaa
    48721 gttgaagagg aagaagagcg tttactggat gatgtctgcg taatggtgga tgttacttcg
    48781 gcagaaattc ggaatattgt cactcgcctg ttagaaaatt ggggtgcaac ctgtatcaca
    48841 cccgatgaaa gattaattag tcaagattat gatatctttt taacggataa tccgtctaat
    48901 cttactgcct ctggcttgct tttaagcgat gatgagtctg gcgtacgaga aattgggcct
    48961 ggtcaattgt gcgtcaactt caatatgagc aacgctatgc aggaagcggt cttacaatta
    49021 attgaagtgc aactggcaca ggaagaggtg acagaatcgc ctctgggcgg agatgaaaat
    49081 gcgcaactcc atgccagcgg ctattatgcg ctctttgtag acacagtacc ggatgatgtt
    49141 aagaggctgt atactgaagc agcaaccagt gactttgctg cgttagccca aacggctcat
    49201 cgtcttaaag gcgtatttgc catgctaaat ctggtacccg gcaagcagtt atgtgaaacg
    49261 ctggaacatc tgattcgtga gaaggatgtt ccaggaatag aaaaatacat cagcgacatt
    49321 gacagttatg tcaagagctt gctgtagcaa ggtagcctat tacatgaaca atatgaacgt
    49381 aattattgcc gatgaccatc cgatagtctt gttcggtatt cgcaaatcac ttgagcaaat
    49441 tgagtgggtg aatgttgtcg gcgaatttga agactctaca gcactgatca acaacctgcc
    49501 gaaactggat gcgcatgtgt tgattaccga tctctccatg cctggcgata agtacggcga
    49561 tggcattacc ttaatcaagt acatcaagcg ccatttccca agcctgtcga tcattgttct
    49621 gactatgaac aacaacccgg cgattcttag tgcggtattg gatctggata tcgaagggat
    49681 cgtgctgaaa caaggtgcac cgaccgatct gccgaaagct ctcgccgcgc tgcagaaagg
    49741 gaagaaattt accccggaaa gcgtttctcg cctgttggaa aaaatcagtg ctggtggtta
    49801 cggtgacaag cgtctctcgc caaaagagag tgaagttctg cgcctgtttg cggaaggctt
    49861 cctagtgacc gagatcgcta aaaagctgaa ccgcagtatt aaaaccatca gtagccagaa
    49921 gaaatctgcg atgatgaagc tgggcgtcga gaacgatatc gccctgctga attatctctc
    49981 ttctgtgacc ttaagtccgg cagataaaga ctaatcatct gtaggccaga taagacgcgt
    50041 tagtgtctta tctggcattt gcaccgattg ccggatgcgg cgtaaacgcc ttatccggcc
    50101 tacgattccc attatttcaa cagattacat taaagtaggc cagataagac gcgtcagtgt
    50161 cttatctggc atttgcactg aatgccggat gcggcgtaaa cgccttatcc gtcctacgaa
    50221 tcccgcgatt tcctgaccct ctcggcatat accgtcagcg tctgttttat cacatccagc
    50281 gttaccggct tcgagaggca gctgtccata ccggactcca gacaccgctg cttctcttca
    50341 gccaacgcat tagcagttac tccgattacc ggcaacgtca gtcccaactg acgaatgcgt
    50401 tgcgtcaagc ggtaaccatc catatttggc atgttgacgt cgctaagcac gatatcaata
    50461 tgattcttac taagtacatt aagcgcatcg acgccatcat tcgcggtttt acattgatag
    50521 cccaacgatc ccaactgatc tgccagcaga cgtcggttaa tcggatgatc atccacgacc
    50581 agaatcatca tatcgtcgtt atcgctgacc gctttgtccg tcgacggcag agcgttagca
    50641 ggatcgtcgc tctccatctc gatcaaataa atacgcgcca acaatgccgg tagctcatgc
    50701 ggagcagcca cactgtgtac ccactcccct ggcgctttct ccagcggaat accaatatgg
    50761 cgacgacaga aggtcactac cgctctgccc tgccattttt tactcactac ctcgtcagtg
    50821 attaacacat cttcgggagt cggttcctgc ccttcgtatg ttgtaacgac gatgccgctg
    50881 cgctgcaaac tggtttccag gaactgacag agcgacgcat tgcggaccgc cagccagcag
    50941 cgtttaccac tcaacccttc cacgcctttt ttctgcgggt actgagcgcc gtacaacgga
    51001 atacgcacgg taaactggct gcccattccc ggttctgaat ctaccgagat atcgccgtcc
    51061 atcatgctga tcagtttttc acaaatcgcc agacccagac cggtcccctg gaagtttcgc
    51121 tgtacgcccg ttccaacctg gaagaaggga tcaaacaagc gcaccacttc tttcgccgga
    51181 atccccacgc cggtatcgcg aacacggata gagagataat cgccatcagc gcgaacatgc
    51241 aaaactatac agccggtatc ggtgaatttt atggcgttac tcaacaggtt ggagatgacc
    51301 tgctgtaaac gcatcgggtc gccatttaag gccactggca catccggttc aataaagcag
    51361 tacaagccta actgcttgcg taccaccagc ggtaaatagt tggcggtgat gtggttcatc
    51421 acttcacgcg gtgaaaactc acgcggttcg atcttcaact gttccgattc aatcttcgag
    51481 aaatcgagaa tatcgctgat aattttcaac aacaggctgg aagagttgtt cattgccgtc
    51541 accagacgat cgacgccttt cggtaactct ttggtttgca acagatccag gttaccgata
    51601 atgccataca gcggcgttcg cagctcatga ctgacggtgg caaggaacat cgattttgac
    51661 tggctcgcct gttccgctgc ttgtgccatc tcctgcaacg actcttccat cttcacgcgc
    51721 gaagaaacat ccaccagcac acaaatggcc acgttttcat tacgatagcg cgaatggacg
    51781 aagctgattt gcagattggt attgttgctg gtcaggacat caacaaaatt gacctgctgc
    51841 ccacagataa tttgcgtcag tcgttggcgg tcctcatgcg taagcatatt gagataggta
    51901 tgcgccagtt cgttacttaa aatattgacg ccatcagcgg tacgcaaaat gcagataccc
    51961 actggcgcgg aggcgacaat cttgcgattg aactgctcat gttcttccag tcgcagggcg
    52021 tcgctttccg ccggaatgaa aatacgtcgc tcgtacatcc gcgcgagagt aaacaatgca
    52081 gctccggcaa gcacattcag caaaattgcg ttaaggatca acatgcgaat gcgttccagc
    52141 accttatcaa ccggcaccga atacacgatg cttagcgatg agggtggcag atttttcttc
    52201 agcaccagct cccggaaccc ttccgtatag ccaaaccagg agcgttcctg catccagcga
    52261 ggatcgccct taattttact ttctggtccg gtaagcgaaa tcagggtatg accattttca
    52321 tcaagaatgg taacccccat cggcaacgta cccggtaaga aaaagttctc catccggatg
    52381 gtctgctcga cacccaaaag cgcctgcaac cggttcgcca gataaactgg cgtcaacgcg
    52441 taaaaatacc cgacgcaagg gcgcggacct tcgctgatcc agtagaggtt actgccgcta
    52501 tcatcttgtg gtgcatttcg atatttattg atgcgttcat gcaacgcttt caacgcggta
    52561 tcgcgttcca ctggcatatc acgcagaccg aaattggcca tgcagaggtt atcactgccg
    52621 attaaaaata cccggttgag atcgtaagcc gcagaaaaat tatcgcgcca gtagcgcata
    52681 aaccacgcca atgactccag agaacctcgc caggtgttac tcattgcgga acaatcggag
    52741 tcggcaaaca gcggttcaaa cgcaggcaca tccgcctgcg tttctcgtcc acgcggggaa
    52801 agcacaccgt tttctgccga taagcgattt tcggcgatgt acttaagctc tttcatcaca
    52861 tcagaggtgc gttgaataaa gcgctgagcc tgatcggaac tcagattaaa ttcctgacga
    52921 atttccgatt ctcgctgatg taacgcatta acgatgtaaa aaacggatga aaaagcaatc
    52981 aacagccaga gcactaacgc caatgctctg aacatgtagc gcgaggcttt cagggttgta
    53041 cgaaaagaag caaggtattt caaaggggag aagctccgcc tcaggtgacc gatggagtgt
    53101 ggttaaggta gcggtaaaag cgtgttaccg caatgttctg tcaccattgc aataatgcgt
    53161 atcgcttaga aaccattgcc ttaaccagga aagccggagt agatggacag ttctcagtac
    53221 gaaaaataag aaatgaccaa tctgccgacg cttgttaatg gttatatcct ggcgtcggca
    53281 gatcgcccag cagtcttatc tgaaaaaaga gagagccagg ttgtgtgaag ggatggatca
    53341 ccagtcaaaa cttttcacca gcgtcagctc gccagcatta cgcatcggta caataaatgt
    53401 ttcctgtttc tcattgaccg atccttcatc ggtgatcagc gtcagttggg cggtcgtcaa
    53461 ttccgtttcg ctgcgcccac catagtagtt gatatacacc tgatagcgcc cgtgaactgg
    53521 tgctggcatg gcgaaaatct cgggtccgta ccccgtcgtg acatccatat ccagtgcacc
    53581 actgtttttc agcacggtgt taccgtacca ggcgtgttcg ccatcgggcg tgacaacatg
    53641 gagatcgaga tcggtattgt ccgtatccca cgagagaacc agccgtaaac gtgcacgaat
    53701 agttcctgta cccggcgttg agtaaaactg cattttttgc cggctttgcc catccgggct
    53761 gatgacctgc acgctgttgc tgccttcagt gaaaatataa gggcgtgcaa atgaaccatc
    53821 ggattcaatt ctctgcggca tactggcacc gttgaccacc agccggccct gctgaacttt
    53881 acccgccgca gcataattct ttattttgcc gcggatctgc gctgagatat tttgatcatc
    53941 tgccatgttg acggacgacg ccgggtaatt gatcgattgt gaaaaactgg catcttcgcc
    54001 ctctgccgga tgccagccag agagcggtgc gtcaatctcg acttcctgca caccttcact
    54061 gtgagcgaca ggcgaaagtg ccaccagtaa caacggaaga aaaatctttc gcatagcatt
    54121 agtccattaa taattgtcgg gtcagcgttt cgatatagtt ctcatcaagc ccggtgggat
    54181 aaccatcaaa ggccaagtgc agatactcat gggttaaatc cagccgatcc tgaagcgtaa
    54241 agaagttacg aataaacagc cgtttctgct gccgatcggt ataggggaaa cctgagacta
    54301 aacgacagac cgcaaacacg tctggttcgt tgtaccccgt ctcgccttgt aatatacgcc
    54361 gccactgcgg catttttttc gccagccagg ctttcgcttt gggtaataac tggcaggttg
    54421 agcgtggagc tccccagcgg ctaaggctgt tgtcgggata agcaaacgcg agtatctggt
    54481 cgtaacgctc cccctgcccc gcctgggccg tggcttgccg ccaggaaagt gtcccttcgg
    54541 tagcgcgact gccatgataa tgaaccggat cgcctgcata gatgagctcc tgcgtccagg
    54601 cggtcatcgt tcgcgccccc gtggtcgctg gcgaagcaga aacacgctgc gtggcgctgc
    54661 tgtcggggat ggtcaggcaa tcaccttccc gattggcgtt ttgctgtaaa aaagtacgta
    54721 tcgccacagt catggctttc gctgcctcag gtggcgtact tttcgcctcg cgatccagaa
    54781 ctctggcaac gtactcttca cgatcgagat gcgactgcaa tttcagtttg cccttttcgc
    54841 tcagtaaggt cgtctcaccg tggctgacaa acgtaatgtg gttaccatta gtaaacgtca
    54901 cacgatagcg cccattaagt acgctgggtt ttactgccgt cgtactcttt tccgctgtga
    54961 ttttcttcag cggatagcgg gcaaacagct ccacctctac acattgccca ctggcaacct
    55021 gcgtcggcac tggcaaaacc cgattaagca ccgtcgcata acgggttagt accgttttgc
    55081 tggtgccact gccagtaacc cacagcggtg taccatcggt taaccagcca gcaaaaccac
    55141 cctgacggat ttcctgttta tcatcggcaa accagctcca ggttttcacc cgtacccgac
    55201 taccgagcat ggatgcgaca ccgattttcg cctcgtccag caccacatcc agtaagactt
    55261 cctgagcttt gttttgcgca ggtaacgtcg ctaatgaatc aagcagcgat ttaaccgtta
    55321 ccgaggtttc tggcttcagc gttgtaagag acgctaacca cgcaggtgcc tgacgctgtt
    55381 gccagtattg cccccacaca tctgcgccaa tatgcaaccg ctgcggcgca aaatagagcc
    55441 cacaggaacg taccagcgcg gtatcgcgcg ttatgctctc cccagcctga caacaataaa
    55501 cctcttccgg ggagtttccg cgacactggt agggctgctc cggttgatgg gtatcctcca
    55561 gccaggcgta aacatacagt ttccacaaac tccctaacgg cgtggtgagc gagtcaggta
    55621 atgctgagac tttggtcacg cctgatgacg acagctgata cagttgatcg tgctgcgcac
    55681 cgcgcagcac cagttgtaac ggcgtctcct ccgcaagcgt tggcagagta accaacgcca
    55741 atagccagac gattctgcgc cagttcactt atttcacctg catcccggtc cattcactcc
    55801 ctgccgcgac actttgctgc gcaggtgcat aggaacgcac ataacgagca ggaggcagaa
    55861 cgaattgccc tttttgcgag aagcgcagca aatggcggaa agtgaccgtt cccgtcagtt
    55921 ctttcaccgg caccatatag gccagttcgc ccatttcatt acgcgctttt tccagcaatt
    55981 gcccctgctg tttcgcggcg ttgggtttat tgactgaaat gccccatgtt gtgcgctcaa
    56041 cgtcagctcc cggcgggagc ggtacttcca cctgaccata gcgcagaact gcatcctgct
    56101 cgctggtaag cgtaatttca tcgagataca gcgcatcgct gtcaatctca ttgctggtca
    56161 ccggctgcag agtaaagctc atctcttctt caccggggat aagccgatac aactggcgtt
    56221 caacggtcac cggaatgtta ctttgttgag ccgtttttgc cggttcacgc cagcggacct
    56281 gcacatttgc ggcgataatt cgtcaccaaa agagagaatg tccggcacac cctaaccaac
    56341 ccaacgccag tcttcgcccc ctccagttaa cttatgttta gcccatgcgc ccgcaggcgc
    56401 aggcaaaaca actggaggca ttgtcgccat atatttcgcc agccagttca tggccagcgc
    56461 gcgctcaata gtggattgct cagcggtcaa accattaaaa tagcggccgt ctgggtagcg
    56521 tcaccaccgc tattgagcag cacaaccgtg tgcgccagcg gctgattgct gtttgccgct
    56581 ttatcccacg catattgctg aacctgtggc atcaccgtgg acttcaactg cgcttttttc
    56641 agcaacgtca tggttaagac gtttgccacc gcatctgcca gtggagattc cggtgtatcg
    56701 aggatcaggc tgtcattgat atcgcggatg tcttcctcag agaaatcttc atctttagtt
    56761 ccgcgccggg cgatagcttc atccccccct ttcaacaacg ttttgcacgg cagattcatc
    56821 tcctgtgccc acgccagcac cagcgcccga tgcaataacg gcatattatc cgcctgctcg
    56881 gcataactgt cgagcatatg ctgccagtat tccggttgtt gcgttacgcc gagcgcctgg
    56941 ctggcctgcc agtcggcgta ccatgcccat gccgtaagga aggcgtcacc attgccatct
    57001 tcaccccacc aggtaaagcg cgctccgggc cccgccagtt gcatcagccg cagacggtta
    57061 tcctgaatca tctgacgaat gtcgttagcg gcggcacttt gatgatcggc aagcgaacgc
    57121 caggcgagac tgagcgggat cagacggcta ccggtgttga tcaccccacc ccacggttca
    57181 tcgactaacg catcaagatt gttgcggaaa atctcctgcg gcgtttcact actttgtagc
    57241 cggatattac tcgcctgctc gggcaacatc agcgcgttct cgccaccgcc gagcatgaca
    57301 tttttctgct gttcaacggg ccagctgttg tccacaaaag acagttttgt gctaatgctg
    57361 tcctgcactt gcccattttg ttgcagttct gcacttaaca agccagattg ctgaatattc
    57421 tgcgtcagcg aaatataatt cgcccctttg tgcagcgtca gcgtctggcg catctcagcg
    57481 cctgcaaatt tagtcaccag cgccaccggt tcgttatcct gctgactgaa gataaacagt
    57541 cctgccgccg gtttgtcgcc cacgcgatac accgttggca tactccactt catgtagaga
    57601 tttttttccg aacgcagata agcacgcccc tgcccgacca gcccgtcgcc gttcatccca
    57661 cgcgcggtga tacgccagcg ggttaacgaa tcaggcatca ggaacgtgaa atacgctttg
    57721 ccttgtttat cggttgtgag tgacggcatc catgccgcgg tatccacctc ttcacgccgt
    57781 ggacgttcaa gcatttttac tcgccgctcg ctgcggttag tcgcgccagg cgcaaccggc
    57841 tcgctggaga gcgcctggtc atagctgata aacgacagac tggagctggt acgcacattg
    57901 ttacgcccca gcggatagaa aaatttgccg atattcggcg cgatttctgg ttgcagcgcg
    57961 tagatcattt catcgaccac gcctaccgtt agctgcgcag aaacaggttt acctttcagc
    58021 gacgaggtta
//
LOCUS       DAJTSI010000007        53659 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000007 DAJTSI010000000
VERSION     DAJTSI010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53659
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.007"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(2..298)
                     /locus_tag="OO327_000366"
     CDS             complement(2..298)
                     /locus_tag="OO327_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCR6017041.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            513..1241
                     /gene="tonB"
                     /locus_tag="OO327_000367"
     CDS             513..1241
                     /gene="tonB"
                     /locus_tag="OO327_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR6017042.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            complement(1281..1679)
                     /gene="yciA"
                     /locus_tag="OO327_000368"
     CDS             complement(1281..1679)
                     /gene="yciA"
                     /locus_tag="OO327_000368"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR6017043.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEVAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(1784..2323)
                     /locus_tag="OO327_000369"
     CDS             complement(1784..2323)
                     /locus_tag="OO327_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR6017044.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(2353..3096)
                     /locus_tag="OO327_000370"
     CDS             complement(2353..3096)
                     /locus_tag="OO327_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR6017045.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(3124..3213)
                     /locus_tag="OO327_000371"
                     /pseudo
     CDS             complement(3124..3213)
                     /locus_tag="OO327_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            3453..4091
                     /gene="ompW"
                     /locus_tag="OO327_000372"
     CDS             3453..4091
                     /gene="ompW"
                     /locus_tag="OO327_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR6017046.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(4151..4657)
                     /locus_tag="OO327_000373"
     CDS             complement(4151..4657)
                     /locus_tag="OO327_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR6017047.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(4703..5203)
                     /locus_tag="OO327_000374"
     CDS             complement(4703..5203)
                     /locus_tag="OO327_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR6017048.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(5289..5468)
                     /locus_tag="OO327_000375"
     CDS             complement(5289..5468)
                     /locus_tag="OO327_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCR6017049.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(5848..6654)
                     /gene="trpA"
                     /locus_tag="OO327_000376"
     CDS             complement(5848..6654)
                     /gene="trpA"
                     /locus_tag="OO327_000376"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR6017050.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(6654..7847)
                     /gene="trpB"
                     /locus_tag="OO327_000377"
     CDS             complement(6654..7847)
                     /gene="trpB"
                     /locus_tag="OO327_000377"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR6017051.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(7859..9220)
                     /gene="trpCF"
                     /locus_tag="OO327_000378"
     CDS             complement(7859..9220)
                     /gene="trpCF"
                     /locus_tag="OO327_000378"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR6017052.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(9221..10816)
                     /gene="trpD"
                     /locus_tag="OO327_000379"
     CDS             complement(9221..10816)
                     /gene="trpD"
                     /locus_tag="OO327_000379"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR6017053.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(10816..12378)
                     /gene="trpE"
                     /locus_tag="OO327_000380"
     CDS             complement(10816..12378)
                     /gene="trpE"
                     /locus_tag="OO327_000380"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR6017054.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(12400..12493)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(12470..12514)
                     /gene="trpL"
                     /locus_tag="OO327_000381"
     CDS             complement(12470..12514)
                     /gene="trpL"
                     /locus_tag="OO327_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCR6017055.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            12652..13533
                     /gene="yciV"
                     /locus_tag="OO327_000382"
     CDS             12652..13533
                     /gene="yciV"
                     /locus_tag="OO327_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR6017056.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            13530..14150
                     /locus_tag="OO327_000383"
     CDS             13530..14150
                     /locus_tag="OO327_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR6017057.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            14251..15123
                     /gene="rluB"
                     /locus_tag="OO327_000384"
     CDS             14251..15123
                     /gene="rluB"
                     /locus_tag="OO327_000384"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR6017058.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(15163..15753)
                     /gene="cobO"
                     /locus_tag="OO327_000385"
     CDS             complement(15163..15753)
                     /gene="cobO"
                     /locus_tag="OO327_000385"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCR6017059.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(15750..16508)
                     /locus_tag="OO327_000386"
     CDS             complement(15750..16508)
                     /locus_tag="OO327_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR6017060.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            16728..17777
                     /gene="sohB"
                     /locus_tag="OO327_000387"
     CDS             16728..17777
                     /gene="sohB"
                     /locus_tag="OO327_000387"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR6017061.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAVLMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(17813..18064)
                     /locus_tag="OO327_000388"
     CDS             complement(17813..18064)
                     /locus_tag="OO327_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR6017062.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            18444..21041
                     /gene="topA"
                     /locus_tag="OO327_000389"
     CDS             18444..21041
                     /gene="topA"
                     /locus_tag="OO327_000389"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR6017063.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALELQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            21251..22225
                     /gene="cysB"
                     /locus_tag="OO327_000390"
     CDS             21251..22225
                     /gene="cysB"
                     /locus_tag="OO327_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR6017064.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            22520..22684
                     /locus_tag="OO327_000391"
     CDS             22520..22684
                     /locus_tag="OO327_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR6017065.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            22687..22854
                     /locus_tag="OO327_000392"
     CDS             22687..22854
                     /locus_tag="OO327_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6017066.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            22968..23063
                     /locus_tag="OO327_000393"
     CDS             22968..23063
                     /locus_tag="OO327_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6017067.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            23227..25902
                     /gene="acnA"
                     /locus_tag="OO327_000394"
     CDS             23227..25902
                     /gene="acnA"
                     /locus_tag="OO327_000394"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR6017068.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELKVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(25966..26556)
                     /gene="ribA"
                     /locus_tag="OO327_000395"
     CDS             complement(25966..26556)
                     /gene="ribA"
                     /locus_tag="OO327_000395"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR6017069.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            26726..27490
                     /gene="pgpB"
                     /locus_tag="OO327_000396"
     CDS             26726..27490
                     /gene="pgpB"
                     /locus_tag="OO327_000396"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR6017070.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            27639..27947
                     /gene="lapA"
                     /locus_tag="OO327_000397"
     CDS             27639..27947
                     /gene="lapA"
                     /locus_tag="OO327_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR6017071.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            27954..29123
                     /gene="lapB"
                     /locus_tag="OO327_000398"
     CDS             27954..29123
                     /gene="lapB"
                     /locus_tag="OO327_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR6017072.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            29316..30053
                     /gene="pyrF"
                     /locus_tag="OO327_000399"
     CDS             29316..30053
                     /gene="pyrF"
                     /locus_tag="OO327_000399"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR6017073.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            30053..30379
                     /gene="yciH"
                     /locus_tag="OO327_000400"
     CDS             30053..30379
                     /gene="yciH"
                     /locus_tag="OO327_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR6017074.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(30505..30723)
                     /gene="osmB"
                     /locus_tag="OO327_000401"
     CDS             complement(30505..30723)
                     /gene="osmB"
                     /locus_tag="OO327_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR6017075.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(30992..31741)
                     /locus_tag="OO327_000402"
     CDS             complement(30992..31741)
                     /locus_tag="OO327_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6017076.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(31831..32004)
                     /locus_tag="OO327_000403"
     CDS             complement(31831..32004)
                     /locus_tag="OO327_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6017077.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            complement(32152..34126)
                     /gene="pdeR"
                     /locus_tag="OO327_000404"
                     /pseudo
     CDS             complement(32152..34126)
                     /gene="pdeR"
                     /locus_tag="OO327_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            34180..34283
                     /locus_tag="OO327_000405"
                     /pseudo
     CDS             34180..34283
                     /locus_tag="OO327_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(34361..36295)
                     /gene="rnb"
                     /locus_tag="OO327_000406"
     CDS             complement(34361..36295)
                     /gene="rnb"
                     /locus_tag="OO327_000406"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR6017078.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(36363..37490)
                     /locus_tag="OO327_000407"
     CDS             complement(36363..37490)
                     /locus_tag="OO327_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCR6017079.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            complement(37634..38422)
                     /gene="fabI"
                     /locus_tag="OO327_000408"
     CDS             complement(37634..38422)
                     /gene="fabI"
                     /locus_tag="OO327_000408"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR6017080.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            complement(38790..39143)
                     /locus_tag="OO327_000409"
     CDS             complement(38790..39143)
                     /locus_tag="OO327_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR6017081.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            complement(39211..40017)
                     /gene="sapF"
                     /locus_tag="OO327_000410"
     CDS             complement(39211..40017)
                     /gene="sapF"
                     /locus_tag="OO327_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR6017082.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(40019..41011)
                     /gene="sapD"
                     /locus_tag="OO327_000411"
     CDS             complement(40019..41011)
                     /gene="sapD"
                     /locus_tag="OO327_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR6017083.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            complement(41011..41901)
                     /gene="sapC"
                     /locus_tag="OO327_000412"
     CDS             complement(41011..41901)
                     /gene="sapC"
                     /locus_tag="OO327_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR6017084.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            complement(41888..42853)
                     /gene="sapB"
                     /locus_tag="OO327_000413"
     CDS             complement(41888..42853)
                     /gene="sapB"
                     /locus_tag="OO327_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR6017085.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            complement(42850..44493)
                     /gene="sapA"
                     /locus_tag="OO327_000414"
     CDS             complement(42850..44493)
                     /gene="sapA"
                     /locus_tag="OO327_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR6017086.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            complement(44806..45051)
                     /locus_tag="OO327_000415"
     CDS             complement(44806..45051)
                     /locus_tag="OO327_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR6017087.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            complement(45185..46570)
                     /gene="puuP"
                     /locus_tag="OO327_000416"
     CDS             complement(45185..46570)
                     /gene="puuP"
                     /locus_tag="OO327_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR6017088.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            complement(46560..46724)
                     /locus_tag="OO327_000417"
     CDS             complement(46560..46724)
                     /locus_tag="OO327_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6017089.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            complement(46873..48291)
                     /gene="puuA"
                     /locus_tag="OO327_000418"
     CDS             complement(46873..48291)
                     /gene="puuA"
                     /locus_tag="OO327_000418"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR6017090.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            48503..49267
                     /gene="puuD"
                     /locus_tag="OO327_000419"
     CDS             48503..49267
                     /gene="puuD"
                     /locus_tag="OO327_000419"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCR6017091.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            49294..49851
                     /gene="puuR"
                     /locus_tag="OO327_000420"
     CDS             49294..49851
                     /gene="puuR"
                     /locus_tag="OO327_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR6017092.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            50001..51488
                     /gene="puuC"
                     /locus_tag="OO327_000421"
     CDS             50001..51488
                     /gene="puuC"
                     /locus_tag="OO327_000421"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCR6017093.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLQTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVRIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            51490..52770
                     /gene="puuB"
                     /locus_tag="OO327_000422"
     CDS             51490..52770
                     /gene="puuB"
                     /locus_tag="OO327_000422"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR6017094.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            52808..>53659
                     /gene="gabT"
                     /locus_tag="OO327_000423"
     CDS             52808..>53659
                     /gene="gabT"
                     /locus_tag="OO327_000423"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HCR6017095.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANI"
BASE COUNT        13447 a        13469 c        13893 g        12850 t
ORIGIN      
        1 ctcagaacac tttcttaaat ggtttcactg aaacgtgttc atagactcct gccgctacgt
       61 acgggtcagc atcggcccag gcctgagctg cttccagcga ttcaaattca gcaataacgg
      121 ttgagccagt aaatcccgca gcccctggat cgttactgtc taccgctggc attggaccag
      181 ctgtcaacaa acgaccttca tcatgcagta actgtaaacg tgctaaatgt gccggacgaa
      241 cggaaaggcg tttttcgagg ctatcagctt tgtcttgagc gtaaataaca tacaacacgg
      301 gcacaactcc ttgttcggga aagttgtaaa gtacgttatt tgaaagggcg aagatctgca
      361 acggaaagat gatgtctttg ttaaggtcat gcataaagta agggtaatta cgccaaaaat
      421 gacattttca ctgatcctga tcgtcttgcc ttattgaata tgattgctat ttgcatttaa
      481 aatcgaggcc tagtttttct actgaaatga ttatgacttc aataaccctt gatttacctc
      541 gccgcttccc ctggccgacg ttactttcgg tctgcattca tggtgctgtt gtggcggggc
      601 tgctctatac ctcggtacat caggttattg aactacctgc gcctgcgcaa ccaatttctg
      661 tcacgatggt tgcgcctgct gatctcgaac cgccacaagc cgttcagccg ccaccggagc
      721 cggtggtaga gccagaaccg gaacctgagc cgatccccga accgccaaaa gaagcaccgg
      781 tggtcattga aaagccgaag ccgaaaccta agccaaaacc gaagccggtg aaaaaggtac
      841 aggagcagcc aaaacgcgat gtcaaacccg tagagtcgcg tccggcatca ccgtttgaaa
      901 atacggcacc cgcgcgcccc acatcaagta cggcaacggc tgcaaccagc aagccggtta
      961 ccagtgtggc ttcaggacca cgcgcattaa gccgtaatca gccgcagtat ccggcacgag
     1021 cacaggcatt gcgcattgaa gggcaggtta aagttaaatt tgacgtcacg ccggatggtc
     1081 gcgtggataa cgtacaaatc ctctcagcca agcctgcgaa catgtttgag cgtgaggtga
     1141 aaaatgcgat gcgcagatgg cgttatgagc cgggtaagcc aggcagtggg attgtggtga
     1201 atatcctgtt taaaattaac ggcaccaccg aaattcagta agcagaaagt caaaagcctc
     1261 cgaccggagg cttttgacta ttactcaaca ggtaaggcgc gaggttttcc ttcaggatca
     1321 accgctacat acttaaataa tgcttctgtc gctttatagc gttgcccgat tggttcagac
     1381 gctacttttt tcacccacac ttcaatatta atgctgaccg atgtcgtccc tttctggaca
     1441 cagcgtgcat agcagcacac cacatcgccg accgcaaccg gccgtaagaa agtcattcct
     1501 tcaacccgca cagtcactac gcgaccgtgg gcaacttctt ttgccagaat agcgccgcca
     1561 atatccattt gtgacattaa ccaaccacca aagatgtcac cattggcatt ggtatcggcg
     1621 ggcatggcta aagtacgtaa aacaagatcg ccctgaggga cgttatgtgt tgtagacatg
     1681 gtaaaaccga cttaaaagga aaatcacagg cgcgatgcta ctatgatttt cctgaggaga
     1741 acagaggaga tacagcgcca gccccgaagg actggcagtc tggttaggat ttatcttcct
     1801 gcggcatgtg gcggtagata tagataccgc ttaacaatgt gaagattaag gtaagggcgg
     1861 tcaggccaaa gactttaaag ttgacccaaa tattttgcgg cagccagaat gcgatgtaga
     1921 tgtttgccag accgcaaagg ataaagaaaa cagcccaggc cagatttagc ttcgaccata
     1981 ccggttgcgg cagcgtgagt tctttactca gcatccgctg aattagcggc tttttcatca
     2041 cccattggct gactaacagg gctcccgcaa acagagcata aatgactgta accttccatt
     2101 taataaactc atcattgtgg aagaacagcg tcaagccacc gaagacgacc accagaacaa
     2161 aagtgatcag ggccatcttc tcaaccttac gaaagcgaac ccagctatag ataagcacaa
     2221 tcgccgtggc gacgatcagc gctgcagtag ccgcatagat gtcataaatc ttgtaaaacg
     2281 cgaaaaagac aaccagcggt aaaaaatcaa gaaactgctt cattttacga ttccgtaatc
     2341 aaatgcttaa ggttattggc gaatcaacat atacaggcgg aacagataga tgagcaatat
     2401 ggcagaaatc aggttgctca aggtgttcgc cagtacggca ccaatttccg gggttaatgc
     2461 ggcaaaagaa gaggcaaaaa gcagcaacag tgtttttgcc agcaaccagc tcagtactgc
     2521 gggtgccacc agacgcatat tcgcccaagt cagccgcata ctgctacgca tcgaggcaaa
     2581 aatgcccatt ttgtcctgta ccagcatcac cggtgccagg gccagtaaaa tggccatgat
     2641 aattcccgga acgaccacca gcatgatgcc aatctgtact aaaagggtag tcagaaaaat
     2701 cagaataaat aacttgggca atatcggcgc actggcacca atagctcgca gcgcactgac
     2761 tctctgaccc gcagacacca gctggataat taatattacg cctccggcga gaatggcgtt
     2821 accgattaat tctgaaaaag tggacgccgc tgaagcctgc agcaaaattt gttgctgttc
     2881 cggtgacata ttctgaacca ggtcgaacaa cccactactg ccgctaacgg gcacgccgtc
     2941 attgagctgc gcaagctgtg catcactggg tgagaaaaca tgccctaaca ccactgtgat
     3001 aaacgcacat agcaacgata ccaacagaat ggtcataaat tgattacgga agaaatttcc
     3061 ggtgtcacgg tatacggact gcgccgtgat agacatgcac tctccttgag ttttatgcag
     3121 gtgttaatta gcgggcaatt gtaccctggt taagcctcgg gtggcagcat caggtcttgt
     3181 atggaaaagc atatctttgt aaagcggagg taattgctgg ccttcaggcg tgggcaaagc
     3241 gcggcaagtg gcgatcgagt taatatttgc gtagagaaaa tatttaaaaa ttgatttaaa
     3301 tcacattaac caggattctc aatgcaactt ctaaattaat ccagatcaat aaatggtgaa
     3361 ttatcatatg taatgtgatc tatgtaggat catttgttac tccaatgtag gtatattcgt
     3421 cacgttttta taaccataac gacggagcgg atatgaaaaa gttaacagtg gcggctttgg
     3481 cagtaacaac tcttctttct ggcagtgcct ttgcgcatga agcaggcgaa ttttttatgc
     3541 gtgcaggttc tgcaaccgta cgtccaacag aaggtgctgg tggtacgtta ggaagtctgg
     3601 gtggattcag cgtgaccaat aacactcaac tgggcttgac gtttacttat atggcgaccg
     3661 acaacattgg tgtggaatta ctggcagcga cgccgttccg ccataaaatc ggcacccggg
     3721 cgaccggcga tattgcaacc gttcaccatc tgccgccaac actgatggcg cagtggtatt
     3781 ttggtgatgc cagcagcaaa ttccgtcctt acgttggggc cggtattaac tacaccacct
     3841 tctttgataa tggatttaac gatcatggca aagaggcggg gctttccgat ctcagtctga
     3901 aagattcctg gggagctgcc gggcaggtgg gggttgatta tctgattaac cgtgactggt
     3961 tggttaacat gtcagtgtgg tacatggata tcgataccac cgccaattat aagctaggcg
     4021 gtgcacagca acacgatagc gtacgcctcg atccgtgggt gtttatgttc tcagcaggat
     4081 atcgttttta attccgcaca aaaacgaccc cgtaatatac ggggtcaata aggacatggt
     4141 ataaagggta ttatttcttc gcttctacgc catcagtttc agaacgaatt aaaaatttct
     4201 cagttgtttg cggaatatgc tgaatcagcc agtcggccat ttgcttttcc tcatttaaaa
     4261 tcgcttcgat gatcggaatt gagactgtat caccggcatt ttttgctgct gctaatagtg
     4321 aggtgtaaca ggtgatttca aattgctcga agacatatcc gccaatagag cctttaacta
     4381 tttcatcaga agggaatata ccaccgattg actgcccaag cgcagccatt ttactcatgg
     4441 aatctttaat gactgaacgt gaaatgtcat tacgatcaag aatagtttcc agttgaacaa
     4501 tctggttttt ggtttcacta agatgttgtt caatacgagc gcgtagttca ggataattat
     4561 ctatacggct ggccatggat tcaagcatag attcggcttg cttttccatt gcgtgggcgt
     4621 cacgtaacca gtcatgataa tgttcaatac gattcatgaa aatgtcctct ttataaaatg
     4681 actaaaagtt aaaattcata tttcaggctt tattttcggc tttcttattt acgttattaa
     4741 gggccagatc agtcagtttg atgtcggtgg ccttttcttc ttccagggtt tctttcagaa
     4801 gcttcgctgc tttacggtaa cctaattgtt cagccagcgt cgctaatgtc ccgtaactgg
     4861 caatctcata atgctcgact ttctgtgctg cggcaatcag tgcggcatca cgcacttcgt
     4921 ttttctcggt actttcgatg acctcattag cttcttcaat aagaccttcc attgccacac
     4981 atttcatgcg cttaattttc agatttgatt ctgattccac aacttgatca attcgttcaa
     5041 tctgtccatg agtttcctcg aggtgcgcat gaaaagcctg acttaatttt tcatttgatg
     5101 ttgctcttgc gagttttgcc agtgcccggg ttaattgttt ttctgcgctg taggtatctg
     5161 aaagcagttg aataaataca tcttcaatgg tcttcatatt catatttttc tccagtgaaa
     5221 tcactgcgag ccatacggcc cgcagatgca attatcttta tcagctgata ttaataatta
     5281 acggtgaatc aggatttgcc tgatttatta ccaccgcttt gttgaccgcc ttttttaccc
     5341 gcttcagatg cgcgttgcgg atcattttta aaattaccgc cgctatgctg accgccttta
     5401 cggcctgcgt cggatgcctt ctcacggtct tcggcgaaat ttcctgaact accacgatgt
     5461 tcggccatgt tatttctccc gttgcgttgc attgtttcat taatatgagt gttgtgtgtc
     5521 gacactcatt aaaattagtc actaatgaga attagtcaaa ttaagcgcaa cgagaagata
     5581 gagggaaata tattttgagg aacattctgg atatattaac aattacctga ggaatatgtg
     5641 acttagagaa aattattgat tttactggtg ttatgttgcg ggattaattt gtttgattat
     5701 attactgttg ggcggaaaaa tgacgtaagt tgacgttcga ccggggtaag cgaaacggta
     5761 aaaagataaa tattaaatga atttaggatt tttccggctt cattaaagaa agttaaaatg
     5821 ccgccagcgg aactggcggc tgtgggatta actgcgcgtc gccgctttca tcggttgtac
     5881 aaaaactttc agtgccgcca gcattttctc tggctcatta atatgttgct cgatgatttt
     5941 aacaatggcc gaaccagaaa tcgcgcccgc agctcctgca tcaatcgctg cttttacctg
     6001 atccggggcg gaaataccaa acccctgcaa tggaggtgca gcgttgtact ctttcagctt
     6061 cgcaaccaga tgattgaggg gtaacgcggc gcggttttct gcgccggtca cgcccgctcg
     6121 tgacagcaaa tatgtgtaac cacgaccgta agaggctatc tggcgcagca ggtcgtcatc
     6181 ggcatttggc gggcagatga agataggtgc gacattatgg cgcaacgcgg cctggcggaa
     6241 gggcgcggac tcttcaactg gcacatcggc aaccagcacc gaatcgacgc cgactttctc
     6301 gcactgggca taaaactcat caatgccttt gttaaacacc agattggcat acatcaacag
     6361 gccaatggga atggtcgggt gtttctggcg aatcagtgcc agcatttcaa aacattgtgc
     6421 cggagtcaca cctgccgcaa aggcgcgcag agtggcgttt tgaatggtcg ggccatccgc
     6481 cagtgggtcg gagaagggga tacctaactc cagcgcgtca gcaccggctt caattagcgt
     6541 atcgataatt ttcaatgact gctcaatgcc cggatcaccg agcgtgacga aaggaacgaa
     6601 tgcgccttct ttgcgctcct tcaactgggc aaacagagat tcgtagcgtt ccatcagatt
     6661 tcccctcgtg ctttcaaaat atcgtgaacg gtgaagatgt ctttatcgcc gcgaccggaa
     6721 aggttaacca ccagtagctg ctctttatcc gggttttcgc gcatcatttt cagcgcatgg
     6781 gccagggcgt gggaggattc cagcgccggg atgatccctt cgtgcaggca cagcgttttg
     6841 aaggcttcaa gggcttcatc atcggtaata gacacgtaat cagcgcgtcc agtgctgtta
     6901 agatacgcgt gttgtggacc gacagacggg aaatccagtc cggcggagat ggagtaagat
     6961 tcttcaatct gcccgtcttc ggtttgcatc atcggcgctt tcataccgaa ataaatgccc
     7021 acgcgaccat gttttagcgg tgcgccgtgc tcgccagttt cgataccgtg accaccaggc
     7081 tccacaccaa tcaggccgac gttggtttca ttgatgaaat cagcaaacat gccgatggca
     7141 ttcgaaccgc cgccaacaca ggcgataacg gcatccggca ggcgaccttc tctttccaga
     7201 atctgcgctt tggtttcttc gccaatcatc cgctgaaact cacggacaat ggtcggataa
     7261 ggatgcgggc cagccgcggt gcccagcata tagtgcgcgg tttcataact gccggaccag
     7321 tcgcgtagcg cctcattaca ggcatctttc agcgtggcgg aaccgctatg taccgggatc
     7381 acttccgcac ccattaagcg catacgaaaa acgttaggcg actggcgttc aacgtctttg
     7441 gcacccatat aaatacggca tttcaggccg agcagggcgc tggcaagggc cgacgccacg
     7501 ccatgctgac cggccccggt ttcggcgatg atttcggttt tacccatccg cttcgccagc
     7561 aacgcctgcc ccagcacctg gttggtttta tgtgcgccgc cgtgcagcaa atcttcacgc
     7621 ttgagataca gcgtggtgtt cgtcccggct gtaatgttct ggcatttggt cagtgcggtt
     7681 ggacgcccgg catagttttt cagcaggtcg ttgaactggg cttgaaattc aggatctttt
     7741 tgcgcgctga caaaagcttc ttccagctgg cgcagagcag gcatcaggat ttgtggcacg
     7801 tacatgccgc caaactcacc aaaatagggg ttaagtaatg ttgtcattgt tcctttcctt
     7861 aatatgcgcg cagcgtctgg aaaaccgagg ccaaaagacg tgcgtctttg atgcccggtt
     7921 gcgactctac agcagaatta aaatcaagtc cggcgcagcc ggtttgtgcc gcttccacgc
     7981 agttatctgc gcctaagccc cccgccagca gaacgttgcc aagcgattga ccatttaata
     8041 gtgaccagtc aaaacgttgc ccgcttccac cctggccgtt gtctaaaaca tatttatcaa
     8101 cgtgctgaaa ctcgcgggcg ggcagggttt caccgacgct taatgctttc cagatggcaa
     8161 catgtgctgg cagagcttca cgcagcgtat cgatatacag ctgatcttca ttgccatgca
     8221 gttgcactgc cgccagcgat aacaccttag ctttgtccac cacatcggca atatcgtgat
     8281 tgcggaacac gccaacatac tgcaacggtg ccgcagccat cacttcctgc gcctgttcaa
     8341 cgttgacgca acgcggtgat gtcgcaacaa aaatcaaccc accgtaaatc gcgcccgcgt
     8401 cataagctgc tttagcatct tgcccacgcg tcaggccaca tactttattc tcacccagca
     8461 acacccggcg cacggcggcg tgcaaatcgt catgggccat caacgccgaa ccaatcagaa
     8521 aaccgttagc gaagtggctt aactcgcgca cctgagcgta agtattgatg ccggattcgc
     8581 tgattaccgt tacgttgtgc cccagtttcg gcgcaagctc gcgggtacgg ttgagatcaa
     8641 tcgacaaatc acgcagatcg cggttgttga tgccaacgac ctttgcccct aatgcaatgg
     8701 cgcgctccag ttcctcttca ttactgactt cggtcagcac ccccatctcc agactgtgag
     8761 cgacggctgc aagctggcga tattggtcgt catccagtac tgaaagcatt aataagcagg
     8821 catcggcctg gtaatagcgc gccagataga tctggtaagg gtcgataatg aagtctttac
     8881 ataaaatcgg ctgcggggcg atttggctga cgatgaggag gaaatcaaag ctcccctgaa
     8941 aatatttctc atcagtcagc accgaaattg ccgaagcgta atgtttataa atggcggcaa
     9001 tgcgtgccgg atcgaaatca tcacggatca cgccttttga cggcgacgct tttttacact
     9061 ccagaataaa cgccgtgcgt gcaccctgta gcgcatcata aaaatgtcgt gtgctcggct
     9121 gaacctcatt ctgaaaactg gccagcggtt gctgctgttt gcgggtttct acccaaatcg
     9181 ccttgtctgc gacgattttc gctaaaacgg tttgcatcat ttaccctcgt gccgccagcg
     9241 cggtaactct gtcgtaagcg gaaccactgc gcagtacctc aagaacggtt tgcgcattgg
     9301 cttgcagatc ttcatggcca tgcaggcgca ttaacatggc gacgttcgca gcgacggctg
     9361 cttcatgggc ggcgtcgcct ttaccttgta acaagcgtgt taaaatgtca cggttttctt
     9421 caggtgttcc gcctgccagt tgctcctggt ggtagggagt caggccaaaa tcttcagcgg
     9481 tcaattgata gctcttaatt ttgccgtcat gcagctcggc aacgattgtc ggcgcgtgta
     9541 atgaaacttc atccatcccg ccgctgtgca ccaccgccgc gcgttgatac cccagcacgc
     9601 gcaaggtttc ggcaatcggc agcaccagtt ccggactata aacaccaatt aacgccagcg
     9661 gcggatgcgc cgggttaatc aatggcccca gcacattgaa cagggtgcgg gttttcagtt
     9721 gctggcgaac cggcatcgcg tggcggaatc cggtgtgata tttcggcgca aagaggaaac
     9781 atacgcctaa ctcgtccagc gcctggcgcg atttattggc gttcatatca agattaatac
     9841 cgaatgccgc cagcagatcc gacgagccgg atttactgga gacgctgcgg ttaccgtgtt
     9901 tcgccacttt cagcccacag gccgcggcga caaacgcact ggcggtagaa atattgatac
     9961 tgttgctgcc gtcaccgcca gtaccgacga tatcagcaaa cagataatcc gggcgcggga
    10021 acggcgctgc gttttccagt agcgcggttg ctgccccggc gatctcgttc gggtgctcac
    10081 cgcgaatttt catgctcacc agcgccgccg ccagttgttc cggcttcagc tcgccacgca
    10141 ccaccgctga aaacagctgg tggctttctt gttggctaag cgtctgcgcc tgatacagtt
    10201 tttccagaat cggttgcagc gtgttggctg gctctagttt ctgctgcgcc caggccagcg
    10261 tttgttccag caggcgagcg ccctgggtgg tgagaatgga ttccggatgg aactggaatc
    10321 cacaaacgcg atccgcatcg tgacgtactg ccatcaccat gccattaaaa tgggcgttga
    10381 tggttaaacc ggccggaatg ttactgccaa ccagcgagtg ataacgcgcc accggcagcg
    10441 ggtttgttaa tccggcaaac atcgcctgac cgtcatgttc aatgctcgac gctttaccgt
    10501 gaagaatttc gcccgcctga ccgacatagc ccccgtaagc ttcgacaatt gcctgatgtc
    10561 cgaggcaaat gccaataatg ggcagcttgc cacgcaagcg ggtgaggagt tccggcatac
    10621 aaccggcttc gctcggcaca ccggggccag gagaaagcat cagtaccgga ttgctcatcg
    10681 tcgccaggcg ttcaattaag gtctgcgccg gaatatggtt gcggtaaatc accacgttat
    10741 gaccattgct gcgcaactga tctgccaggt tgtacgtaaa agagtcgata ttatcgagca
    10801 gcagaatgtc agccatcaga aagtctcctg tgcatgatgc gcggtggcaa tagcgcgcag
    10861 tacagcgcgg gctttgttac gggtttcgtc ggcttccgac tgcggaacag aatcaaggac
    10921 tacaccagca cccgcttgca cggtggcgat accgttttcc accagcgccg agcggatcac
    10981 aatgcaggtg tcgagatcgc catgcgcggt gaaataacct accgcgccgc cgtagctgcc
    11041 gcggcgacga ccttccgcct cggcaattaa ctgcatagcg cgtactttcg gcgcaccgct
    11101 taacgtcccc atattcatac aggcgcgata agcgtgcagg gcgtcaagat cgtgacgcag
    11161 ttcgccgact acgcgagaga cgaggtgcat cacataggaa taacggtcaa ctttggtgag
    11221 atcggcgatg tagcggctgc caggggtgca aatgcgtgcc agatcattac gggcgagatc
    11281 aaccagcatc agatgttcag acagctcttt atggtcagta cgcatttcca gttcaatacg
    11341 gctgtcgaga tctctgtcca gtgaaccatc ggcgcgacga ccgcgtgggc gtgttccggc
    11401 aatcgggtag atctcaatct ggcggctggt ggcatcatac ttgagcgagc tttccggcga
    11461 cgcgccaaat agggtgaaat cattatcctg cataaaaaac atgtacgggc tgggattact
    11521 ctttttcagc acgtaatagg ccgccagcgg tgacgggcag ggcagagaga aacggcgaga
    11581 tggcaccacc tggaaaattt ctccagcgcg aatcgctttt tgcaacaaac gcactacgcc
    11641 accgaactct tcatcgctct gattacattc acaacgcata tgcggcacgg aaaccactgg
    11701 cagcggcggc gcggcttcgg tcagttgctg acgtagttcg ttcaggcgag cagtgagacg
    11761 ttgtttttct tcttcattcg gagcaaacag gctggcctga atacgggtgc tttttttctg
    11821 atggtcaatc accatcagcg tttcagcgag ataaaaacag aaatcagggc agttattttc
    11881 cgctgacagt tgcggtaaat cttcaaatcc cgccacaagg tcataagaga acaggccgcc
    11941 gaagaacatt gcttctcgtt cttccttcgg tacattcaac agattctgca ataaacggaa
    12001 agcgtcaaaa accgaaaggg agcataagcg ggcgtcttca tccagcagtg gactgacagg
    12061 ggggaagcgc agcacacggc agtttggtga ttgttcactt tccacacccg caggcagggc
    12121 gttatccagt agtgccagca gggcttcgcc attgccggaa agtgcctgaa ttgtgacggt
    12181 gtcacctaat gctgtaatgc gcagcgcact gtctatcagc agcaggcttt ttaaatcatc
    12241 tttgctgtcg atatctgcgg attccagcag cagcgttgcc ggacgattcc cacacaactg
    12301 gtgaaaaagc gcagtcgggt tgtcgcgata agcgccttcg caggttagca gttcgagagt
    12361 cggtttttgt gtttgcattg ttattctcta attttgttca aaaaaaagcc cgctcattag
    12421 gcgggctggg tatctgattg ctttacgcat ggtgaataca ctgcccgttt caggaagtgc
    12481 gccaccaacc tttcagtacg aaaattgctt tcattgtcga tacccttttt acgtgaactt
    12541 gcgtactagt taactggttc gatgattaat tgtcaacagc tcatttcaga atatttgcca
    12601 gacccgttat gatgtcggcg caaaaaacat tatccagaac gggagtgcgc cttgagcgac
    12661 acgaattatg cagtgattta cgacctgcac agccatacca cagcttccga tggctgcctg
    12721 acgccagaag cattggtgca ccgtgcagtc gagatgcgcg tcggcaccct ggcgatcacc
    12781 gaccatgaca ccacagctgc aatcgcgcca gcaagagagg aaatttcacg ttcgggactg
    12841 gcgctgaatc ttattcccgg cgtggaaatt tccacggtct gggaaaatca tgaaattcat
    12901 attgtcgggc tgaatattga tattactcat ccgctaatgt gtgagttcct cgcgcagcag
    12961 acagaacggc gcaatcagcg ggcgcagcaa attgccgaaa gacttgaaaa agcgcaaata
    13021 cctggggcgc tggaaggcgc acaacgactg gcgcagggcg gcgcagtgac gcgcggtcat
    13081 tttgcgcgtt tccttgtgga gtgcggcaaa gccagttcaa tggcagatgt ctttaaaaag
    13141 tatctggcgc gcgggaaaac cggatacgtt ccgccacagt ggtgtacaat agaacaagct
    13201 attgatgtca ttcatcattc tggcggtaag gcggtgctag ctcatcctgg gcggtacaat
    13261 cttagtgcta aatggctgaa aagattggtt gcgcattttg ccgaacacca cggcgacgcg
    13321 atggaagtcg cgcagtgcca gcaatcgccc aatgaacgca cccagctggc cacacttgcg
    13381 cgtcagcatc atttatgggc atcacaagga tctgattttc atcagccatg cccgtggatc
    13441 gaactgggtc gtaaactctg gctgcccgca ggcgttgaag gcgtctggca gctatgggaa
    13501 cagccgcaga acaccacaga gagggaatta tgagccagtt tttttatatt catcctgata
    13561 acccgcagca acgtctgatc aaccaggcgg tggagatcgt gcgtaaaggc ggggtgattg
    13621 tctatccaac tgattccggc tatgcgctcg gctgtaaaat tgaagataaa aacgccatgg
    13681 agcgtatttg tcgtattcgc cagctgccgg acggtcacaa ctttaccctg atgtgtcgcg
    13741 atctttctga actctcgacc tattcattcg tggataacgt tgcgtttcgt ttgatgaaaa
    13801 acaacacgcc gggcaactat accttcatcc tgaaggggac gaaggaagtg ccacgccgtc
    13861 tgttgcagga gaaacgcaaa accatcggta tgcgtgtgcc ttctaacccg attgcgcagg
    13921 cgttgcttga agcactgggc gaaccgatgc tttccacttc gctaatgctg ccaggcagcg
    13981 aatttaccga atccgatccc gaagaaatta aagaccgtct ggaaaaacag gtagatttga
    14041 ttattcacgg cggctatctc gggcagaaac caacaacggt tattgatctt accgatgata
    14101 cgccggtcgt ggtgcgtgaa ggcgtaggtg atgtgaagcc tttcttataa gtgcgtaact
    14161 ctgtatacta cgcggcctta aaatggcgct atacggcgcc attgattctc ctgttcgacg
    14221 cctgtgaagg cgacaccaaa aggaagctct atgagcgaaa aattacagaa agtgctggcc
    14281 cgcgctggcc acggctcccg ccgtgaaatc gaatctatta ttgaagccgg tcgtgtgagt
    14341 gttgatggca aaattgctaa actcggcgat cgcgttgaag ttacccctgg gctgaaaatt
    14401 cgtatcgatg gtcatctgat ttcggtacgt gagtccgctg aacaaatttg tcgcgttctg
    14461 gcctattaca aaccggaagg tgagttgtgt acccgtaacg atccggaagg gcgtccgacg
    14521 gtgtttgacc gtctgccaaa gctgcgtggc gcacgctgga ttgccgtagg tcgtctggac
    14581 gttaacacct gtggtctgct gctgttcacc accgatggtg aactggcaaa ccgtttaatg
    14641 catccaagcc gtgaagttga acgtgaatac gccgtgcgtg tatttggtca ggttgacgac
    14701 gcgaaactgc gtgatttgag tcgtggcgtg caactggaag atggtccggc agcttttaaa
    14761 accatcaagt tcagcggcgg cgaagggatc aaccagtggt acaacgtgac tctgaccgaa
    14821 ggccgtaacc gtgaagttcg tcgtctgtgg gaagcggttg gtgtgcaggt gagccgcctg
    14881 atccgtgttc gttacggtga tatcccactg ccaaaaggtc tgccacgcgg tggctggacg
    14941 gagctggacc tcgcccagac taactatctg cgcgaactgg tggagcttcc gccagaaacc
    15001 agctctaaag tcgctgtaga aaaagaccgt cgtcgcatga aggctaatca gattcgtcgt
    15061 gcggtgaaac gtcacagtca ggtgagcggc agtcgtcgtt ctggcgggcg taataacggt
    15121 taatcaaagt atgccggacg tcatatccgg catttttaca gattaataat caatccctat
    15181 ttgcgcctta acaccggcat caaacgcatg tctgatgggg cgcagctcac ttaccgtatc
    15241 cgccagttca agaatatccc gatgacagcc tcgacccgtg ataatcaccg tctgttgatg
    15301 tggacgttca tttaacgcct gcaccacttc ttccagtggc aaatagtcat acgccaccat
    15361 atacgtcagt tcatcaagca aaaccatatc cagtgaggaa tcagcaagca tccgctttgc
    15421 atgttgccag acttcgcggc aggcggcggt atcagactcg cggttttgtg tatcccaggt
    15481 aaagcccgtt gccatcacct gaaactcaac gccatgtggc tccagcagat tgcgttcgcc
    15541 attaggccag gtgcctttaa taaactgcac gacgcccact ttttttccgt gaccaactgc
    15601 gcgtgttgcc gtaccaaatg ccgcggtggt tttgcctttt ccattgccgg taaagacgat
    15661 gataataccg cgttcatcct gggcctgggc cacacgagca tctacttttt ctttcactcg
    15721 ctgctgacgc tgttggtagc gttcatcact cattgggaaa ttcctggttt acggcccggt
    15781 tgggcgtcaa aggtcatgcc ggttttacgg cggctgtcat cgcccatcag ccagaggtag
    15841 agcggcatga tatcagcggg tgttttaagt ttctgtggat cttcggtcgg gaaggcgctt
    15901 gcgcgcattg cggtgcgcgt accgcctggg ttaatgcagt tgacgcgcag gcgctgctga
    15961 tattcatcgg ccagtacctg catcatccct tcggtggcaa atttcgacgc tgcatatgca
    16021 ccccagttgg ctcgtccctg acgtccaacg cttgatgaag taaagaccag tgaaccggcg
    16081 tccgatttga gtaataaagg aagcagtgcc tgggtgagca taaaggtggc attaacgttg
    16141 atctgcatga cgtcctgcca gacctgcgga ttttgttcgc tcattgggca aacatcgccg
    16201 agcaatccgg cattatgcaa aacaccatcc agacgcggat aattaacgac aatgcgctgt
    16261 gccagttgtt ggcaattttc ggacgtgcag gtcagcaaat cgagaataaa ccactgtggc
    16321 tgacgcccag tttcttcgtt tatgtggctg gctacctgac gtaatttttc ttcattacgg
    16381 cccaacagaa tcactgtcgc accatagcgt gcatacgtca tcgcggcttc acgaccaatc
    16441 ccatcgctgg ctcccgtcac caggataatg cgatcattga gtaaatcttg ttttggctgg
    16501 taatgcatgg ctactcctca acgacgttgt ctgtcgcgct gccagtgtac tttatgactt
    16561 tggggcttta tgcctgaaac agaagggcat ttcaatcagc ctggggcgat aacaccgcaa
    16621 aatggatact ttgtcatact ttcgctgcaa taacatctct gcgagacggc ttaacatgcc
    16681 tgttgtaaac tgtgagccaa agcgttgttt aaccaaggtg gggactcgtg gaattgttgt
    16741 ctgaatatgg tttgtttttg gctaaaatcg ttaccgttgt gctagcgatt gcggcgattg
    16801 ccgccattat tgtcaatgtt gctcaacgta ataaacgcca gcgtggcgag ttacgggtca
    16861 acaatctcag cgaacagtat aaggagatga aagaagaact ggccgcggtg ctgatggact
    16921 cacatcagca aaaacagtgg cacaaagcgc agaagaaaaa gcacaagcaa gaagcgaaag
    16981 cagcaaaagc gaaagccaaa ctgggcgagg tggcaactga cagtaaacct cgcgtctggg
    17041 tgctggattt taaaggcagc atggacgccc atgaagtgaa ctcgctacgt gaagagataa
    17101 cggcggtact cgcagcattc aaaccgcagg atcaggttgt gctccgtctg gaaagccctg
    17161 gtggcatggt gcatggttac gggttggcgg cttcgcagct gcagcgtctg cgtgataaaa
    17221 acattccttt aactgttacg gtagacaaag tcgctgccag cggcggttac atgatggcct
    17281 gtgtggcgga caaaattgtt tccgcaccgt ttgctattgt gggttccatt ggggtggtgg
    17341 cgcaaatgcc caactttaac cgcttcctga aaagcaaaga tattgatatc gaactgcaca
    17401 ccgccgggca gtataagcgc acgctgacct tgctgggtga aaataccgaa gaagggcggg
    17461 agaaattccg cgaagagctg aacgaaacgc atcagttatt taaagatttt gtgaagcgta
    17521 tgcgtccgtc tctggatatt gaacaggtgg caacgggtga acactggtac ggacaacagg
    17581 cggtagagaa aggcctggtt gatgaaatca acaccagtga tgaagttatt cttagcctga
    17641 tggaaggccg tgaagtggtt aatgtacgct atatgcagcg taaacgactc attgaccgat
    17701 tcaccggcag cgcggcagag agcgccgatc gattgttgct acgctggtgg cagcggggtc
    17761 aaaagccatt gatgtaaaag acaaacgcga ggctaagacc tcgcgttttg ctttaatcaa
    17821 ccagatgata tttttctgaa agcacatggg ccaggtgttt gaacatatta aacaccgcgg
    17881 tgcttttggc tgttggcaat ccttgttcat ctaaaaagta gtcgccggta aataccagca
    17941 cgccattacg ctgcgtgacg ccggtggctt caatccctgc gagcgtatcc tcatgctcac
    18001 gaatgatttt gttggcctct ttcaacagcg tttcgcggtc gatgggttgt gtctctttgt
    18061 tcattattta ctccttaaac aaggacatta gtctacgcca ggcatggctt gcagacaaat
    18121 ataccacgct ggtggcaaga gcgccttact ggcaactttg gattttgcat gctaataaag
    18181 ttgcgtatcg gattttatca ggtacagtgt gacgctttcg tcaatctggc aatagatttg
    18241 cttgacattc gaccaaaaat ccgtcgtgct atagcgcctg taggccaaga cctgttaact
    18301 cagtcacctg aattttcgtg aacagagtca cgacaagggg ttgatatccg cagagagcga
    18361 gtccatatcg gtaactcgtt gccagtggaa ggtttatcaa cgtgcgacgc attcctggaa
    18421 gaatcaaatt aggtaaggtg aatatgggta aagctcttgt catcgttgag tccccggcaa
    18481 aagccaaaac gatcaacaag tatctgggtt gtgactacgt ggtgaaatcc agcgtcggtc
    18541 acatccgcga tttgccgacc agtggctcag ctgccaaaaa gagtgccgac tctacctcca
    18601 ccaagacggc taaaaagcct aaaaaggatg aacgtggcgc tctcgtcaac cgtatggggg
    18661 ttgacccgtg gcacaattgg gaggcgcact atgaagtgtt gcctggtaaa gagaaggtcg
    18721 tctctgaact gaaacaactg gctgaaaaag ccgaccacat ctatctcgca accgaccttg
    18781 accgcgaagg ggaagccatt gcatggcacc tgcgggaagt gattggtggt gatgatgcgc
    18841 gctatagccg agtggtgttt aacgaaatta ctaaaaacgc gatccgccag gcatttaaca
    18901 aaccgggtga gctgaatatt gatcgtgtta atgcccagca ggcgcgtcgc tttatggacc
    18961 gcgtggtggg gtatatggtt tcgccgctgc tgtggaaaaa gatcgctcgt ggtctgtctg
    19021 ccggtcgtgt gcagtcggtg gcggtccgcc tggtggtaga gcgtgagcgt gaaattaaag
    19081 cgttcgtacc ggaagagttc tgggaagtcg atgccagcac gactacgcca tctggtgaag
    19141 cgttggagtt gcaggtgact catcagaatg acaaaccgtt ccgtccggtc aacaaagaac
    19201 aaactcaggc tgcggtaagt ctgctggaaa aagcgcgcta cagcgtgctg gaacgtgaag
    19261 acaaaccgac aaccagtaaa cctggcgctc cttttattac ctctacgctg caacaagctg
    19321 ccagcacccg tcttggtttt ggcgtgaaaa aaaccatgat gatggcgcag cgtttgtatg
    19381 aagcaggcta tatcacttac atgcgtaccg actccactaa cctgagtcag gacgcggtaa
    19441 atatggttcg cggttatatc agcgataatt ttggtaagaa atatctgccg gaaagtccga
    19501 atcagtacgc cagcaaagaa aactcacagg aagcgcacga agcgattcgt ccttctgacg
    19561 tcaatgtgat ggcggaatcg ctgaaggata tggaagcaga tgcgcagaaa ctgtaccagt
    19621 taatctggcg tcagttcgtt gcctgccaga tgaccccagc gaaatatgac tccacgacgc
    19681 tgaccgttgg tgcgggcgat ttccgcctga aagcacgcgg tcgtattttg cgctttgatg
    19741 gctggacaaa agtgatgcct gcactgcgta aaggcgatga agatcgtatc ttaccggcag
    19801 tcgataaagg cgatgctctg acgctcgttg aactaacacc agcccagcac tttaccaagc
    19861 cgccagcccg tttcagtgaa gcatcgctgg ttaaagaact ggaaaaacgt ggtatcggtc
    19921 gtccgtctac ctatgcgtcg atcatttcga ccattcagga tcgtggctat gtgcgagtag
    19981 aaaatcgtcg tttctatgcg gaaaaaatgg gcgaaatcgt caccgatcgc cttgaagaaa
    20041 atttccgcga gttaatgaac tacgacttta ccgcgcagat ggaaaacagc ctcgaccagg
    20101 tggcaaatca cgaagcagag tggaaagctg tactggatca cttcttctcg gatttcaccc
    20161 agcagttaga taaagctgaa aaagatccgg aagagggtgg tatgcgcccg aaccagatgg
    20221 ttctgaccag cattgactgc ccgacttgtg gtcgcaaaat ggggattcgc acagcgagta
    20281 ccggggtatt ccttggctgt tctggctatg cgctgccgcc gaaagagcgt tgcaaaacca
    20341 ccattaacct ggtgccggaa aacgaagtgc tgaacgtgct ggaaggcgaa gatgctgaaa
    20401 ccaacgcgct gcgcgcaaaa cgtcgttgcc cgaaatgcgg cacggcgatg gacagctatc
    20461 tcatcgatcc gaaacgtaag ttgcatgtct gtggtaataa cccaacctgc gacggttacg
    20521 agatcgaaga gggcgaattc cgcattaaag gttatgacgg cccgatcgtt gagtgtgaaa
    20581 aatgtggctc tgaaatgcac ctgaaaatgg ggcgtttcgg taaatacatg gcctgcacca
    20641 acgaagagtg taaaaacaca cgtaagattt tacgtaacgg tgaagtggca ccaccgaaag
    20701 aagatccggt gccattacct gagctgccgt gcgaaaaatc agatgcttat ttcgtgctgc
    20761 gtgacggtgc tgccggtgtg ttcctggctg ccaacacttt cccgaaatcg cgtgaaacgc
    20821 gtgcgccact ggtggaagag ctttatcgct tccgcgaccg tctgccggaa aaactgcgtt
    20881 atctggccga tgcgccacag caggatccgg aaggtaataa gaccatggtt cgctttagcc
    20941 gtaaaaccaa acagcaatat gtctcttcgg aaaaagacgg aaaggcgact ggctggtcag
    21001 cattttatgt tgatggcaaa tgggttgaag gaaaaaaata acctttaatt ctgtcaggtt
    21061 tttataaaca aagggtcgcg aaagcggccc ttttttattg catattattt tttcttcaca
    21121 cctatacact aaggctataa atgatatagt ggttatagtt agcacctttt ttattattaa
    21181 atcgtattag tcacccgcca ggtgtgacga aaaaacgatg ttctgatggc gtctaagtgg
    21241 atggtttaac atgaaattac aacaacttcg ctatattgtt gaggtggtca atcataacct
    21301 gaatgtctca tcaacagcgg aaggacttta cacatcacaa cccgggatca gtaaacaagt
    21361 cagaatgctg gaagacgagc taggcattca aattttttcc cgaagcggca agcacctgac
    21421 gcaggtaacg ccagcaggac aagaaataat tcgtatcgct cgcgaagtcc tgtcgaaagt
    21481 cgatgccata aaatcggttg ccggtgagca cacctggccg gataaaggct cgctgtatat
    21541 cgccaccacg catacccagg cacgctacgc gttaccaaac gttatcaaag gctttattga
    21601 gcgttatccc cgcgtttctt tgcatatgca ccagggcccg ccgacacaaa ttgctgatgc
    21661 cgtctctaaa ggcaatgccg atttcgctat cgccacagaa gcgctgcatc tgtatgaaga
    21721 tttagtgatg ttaccgtgct accactggaa tcgggctatt gtagtcactc cggatcaccc
    21781 gctggcaggc aaaaaagcca ttaccattga agaactggcg caatatccgt tggtgacata
    21841 taccttcggc tttaccggac gttcagaact ggatactgcc tttaaccgcg cagggttaac
    21901 gccgcgtatc gttttcacgg caacggatgc tgacgtcatt aaaacttacg tccggttagg
    21961 gctgggggta ggggtcattg ccagcatggc ggtggatccg gtcgccgatc ccgaccttgt
    22021 gcgcgttgat gctcacgata tcttcagcca cagtacaacc aaaattggtt ttcgccgtag
    22081 tacttttttg cgcagttata tgtatgattt cattcagcgt tttgcaccgc atttaacgcg
    22141 tgatgtcgtt gatgcggctg tcgcattgcg ctccaatgaa gaaattgagg tcatgtttaa
    22201 agatataaaa ctgccggaaa aataatttct tgcgttattt tcggcacctt ttatgtagcg
    22261 aaggtgccgg aatatattct cttttgttac ttatttctat accgtgcaca atatttttat
    22321 ccctcatatt tatagggtaa attacctgta aagtagtgtc taattacccg cctcgcaaat
    22381 tccagttttc aaatatctat ttcaggtcaa aagattgaat tattcactgg agacgattcg
    22441 taaattcgct ggatttttga ctaaagtttc tttaggattt gtctcatcgt atgattaatt
    22501 acaccaatca ttcaacgtct tgctgataag tgatggcgat tatatgaggt tagcaatgcc
    22561 ttctggaaat caggaacccc gccgtgatcc tgaattaaaa cgtaaagcct ggctggcggt
    22621 ttttcttggt tctgcacttt tctgggtggt tgtcgcactg ctgatttgga aagtgtgggg
    22681 ataactatgg tcggtcagga gcaactggag tcgtcaccat tatgccagca tagtgacaat
    22741 gaaccagaaa cgaaacggga atgttccgtc gttattccag acgactggca actaacatcg
    22801 cagcagcaag cctttataga actgtttgct gaagatgatc agccgaaaca ataattatca
    22861 tcattcttat tacccatttt taatgaatta aagggctttt aatacaccgc agcaataaca
    22921 gcttgagtta tctcaacaca aaataataac cgttaagggt gtagcctatg atcaacacaa
    22981 atatgaaata ttggtcctgg atgggcgcgt tttctctgtc gatgctcttc tgggccgaac
    23041 tcctctggat cattactcac tgatccttga ccccgctgcg gcggggttgt catttgcttt
    23101 gccacaaggt ttctcctctt ttatcaattt gggttgttat caaatcgtta cgcgatgttt
    23161 gtgttatctt taatattcac cctgaagaga atcagggctt cgcaaccctg tcattaagga
    23221 ggagctatgt cgtcaaccct acgagaagcc agtaaggaca cgttgcaggc caaagataaa
    23281 acttaccact actacagcct gccgcttgct gctaaatcac tgggcgatat cacccgtcta
    23341 cccaagtcac tcaaagtttt gctcgaaaac ctgctgcgct ggcaggatgg taactcagtt
    23401 accgaagagg atatccacgc actggcggga tggctgaaaa atgcccatgc tgaccgtgaa
    23461 attgcctacc gcccggcaag ggtgctgatg caggacttta ccggcgtacc tgccgttgtt
    23521 gatctggcgg caatgcgcga agcggttaaa cgcctcggtg gcgatactgc aaaggttaac
    23581 ccgctctcac cggtcgacct ggtcattgac cactcggtga ccgtcgatcg ttttggtgat
    23641 gatgaggcat ttgaagaaaa cgtacgcctg gaaatggagc gcaaccacga acgttatgtg
    23701 ttcctgaaat ggggaaagca agcgttcagt cggtttagcg tcgtgccgcc aggcacaggc
    23761 atttgccatc aggttaacct cgaatatctc ggcaaagcag tgtggagcga attgcaggac
    23821 ggtgaatgga ttgcttatcc ggatacactc gttggtactg actcgcacac cactatgatc
    23881 aacggccttg gcgtgctggg gtggggcgtg ggtgggatcg aagcagaagc cgccatgtta
    23941 ggccagccgg tttccatgct tatcccggac gtagtgggct ttaaacttac cggaaaatta
    24001 cgtgaaggta ttaccgccac agacctggtt ctcactgtta cccaaatgct gcgcaaacat
    24061 ggcgtggtgg ggaaattcgt cgaattttat ggtgatggtc tggattcact accgttggcg
    24121 gatcgcgcca ccattgccaa tatgtcgcca gaatatggtg ccacctgtgg cttcttccca
    24181 atcgatgcag taaccctcga ttacatgcgt ttaagcgggc gcagcgaaga tcaggtcgag
    24241 ttggtcgaaa aatatgccaa agcgcagggc atgtggcgta acccgggcga tgaaccgatt
    24301 tttaccagta tgttagaact ggatatgaat gacgttgaag cgagcctggc agggccaaaa
    24361 cgcccacagg atcgcgttgc actgcccgat gtaccaaaag catttgccgc cagtaacgaa
    24421 ctgaaagtga atgccacgca taaagatcgc ctgccagtcg attatgttat gaacggacat
    24481 cagtatcagt tacctgatgg cgctgtggtc attgctgcga taacctcgtg caccaacacc
    24541 tctaacccaa gtgtgctgat ggccgcaggc ttgctggcga aaaaagccgt aactcttggc
    24601 ctcaagcggc aaccgtgggt caaagcgtcg ttggcaccgg gttcgaaagt cgtttctgat
    24661 tatctggcaa aagcgaaact gacaccgtat ctcgacgaac tggggtttaa ccttgtggga
    24721 tacggttgta ccacctgtat tggtaactct gggccgctgc ccgatcctat cgaaacggca
    24781 atcaaaaaag gcgatttaac cgtcggtgcg gtgctgtccg gcaaccgtaa ctttgaaggc
    24841 cgtatccatc cgctggttaa aactaactgg ctggcctcgc caccgctggt ggttgcctat
    24901 gcgctggcgg gaaatatgaa tatcaacctg gcttctgagc ctatcggcca tgatcgcaaa
    24961 ggcgagccgg tttatctgaa agatatctgg ccatcggcac aagaaattgc ccgtgcggta
    25021 gaacaagtct ccacagaaat gttccgcaaa gagtacgcag aagtttttga aggcacagca
    25081 gagtggaagg aaattaacgt cacacgatcc gatacctacg gttggcagga ggactcaacc
    25141 tatattcgct tatcgccttt ctttgatgaa atgcaggcaa caccagcacc agtggaagat
    25201 attcacggtg cgcggatcct cgcaatgctg ggggattcag tcaccactga ccatatctct
    25261 ccggcgggca gtattaagcc cgacagccca gcgggtcgat atctacaagg tcggggtgtt
    25321 gagcgaaaag actttaactc ctacggttcg cggcgtggta accatgaagt gatgatgcgc
    25381 ggcaccttcg ccaatattcg catccgtaat gaaatggtgc ctggcgttga aggggggatg
    25441 acgcggcatt tacctgacag cgacgtagtc tctatttatg atgctgcgat gcgctataag
    25501 caggagcaaa cgccgctggc ggtgattgcc gggaaagagt atggatcagg ctcaagccgt
    25561 gactgggcgg caaaaggacc tcgtctgctt ggaattcgtg tggtgattgc cgaatcgttt
    25621 gaacgaattc accgttcgaa tttaattggc atgggcatcc tgccgctgga atttccgcaa
    25681 ggcgtgacgc gtaaaacgtt agggctaacc ggggaagaga agattgatat tggcgatctg
    25741 caaaatctac aacctggcgc gacggttccg gtgacgctta cgcgcgcgga tggtagccag
    25801 gaagtcgtac cctgccgttg tcgtatcgac accgcgacgg agttgaccta ctaccagaac
    25861 gacggcattt tgcattatgt cattcgtaat atgttgaagt aacaagtatt tgcttgccgg
    25921 ttattttgct tccggcaagc aaatgaatta cacgatgcaa gagggttatt tgttcagcaa
    25981 atggcccatt ttctcggctt tggtatcgag ataatgttcg ttattggggt tacgacctac
    26041 aatcaatggt acgcgttcaa caatattaat ccctgcttcg gtcagaattt cgactttttt
    26101 cgggttattg gttaacaagc ggacttcatt gacgccaagg agtttgaaca tatcagcgca
    26161 aagagtgaag tcgcgctcat cagcggcgaa gcctaactgg tggttagcct ctacggtatc
    26221 gtaaccttga tcctgcagtg cgtaagcgcg gattttattc agcagaccaa tgttacgacc
    26281 ttcctgacgg tgatacagca aaataccacg gccttcctcg gcaatttgcg tcaatgccgc
    26341 ttcgagctgg aagccacaat cgcagcgcaa gctgaacagg gcgtcaccgg tcagacattc
    26401 ggaatggacg cgcgcaagta ccggggtatg cccggaaata tcgccataga ctagcgcaac
    26461 atgatcgtgt ccggttgcca gttcttcaaa tcccaccatc aggaaatcgc cccatggggt
    26521 tggcagtttg gcttctgcca cacgtttaag ctgcatgaaa ttctccagat aatgctggtt
    26581 ctgtattggc ttattttgcc ataacgagaa ggggttcacc taatcacgac gcgtcgatcg
    26641 ttcacggaat ggcacaaatc tgtcaatttt tcttggaact ggcattttca gttatgattg
    26701 tgggacttat caaaaaggag aggccatgcg ttcgattgcc cgacgtaccg cagtgggagc
    26761 tgcactattg cttgtcatgc cagtagccgt atggatttct ggctggcgtt ggcaacctgg
    26821 agaacaaagt tggctactaa aagcggcttt ttgggttact gaaactgtca cccagccctg
    26881 gggcgtcatt acacatttga ttttgttcgg ctggtttctc tggtgtctgc gttttcgcat
    26941 taaggctgcc tttgtattat ttgccattct ggcggcggca atccttgttg gacaaggcgt
    27001 taaatcctgg atcaaagaca aagtacagga accacgacct tttgttatct ggttggaaaa
    27061 aacacatcat attccggttg atgagttcta cactttaaag cgagcagaac gcggaaatct
    27121 agtaaaagaa cagttggctg aagagaaaaa tattccacaa tatttgcgtt cacactggca
    27181 gaaagagacg gggtttgcct ttccttccgg tcacacgatg tttgctgcca gttgggcact
    27241 gctggccgtt ggtttgctgt ggccgcgtcg gcgaacgtta accattgcta tcttgttggt
    27301 ctgggcaacg ggagtcatgg gaagccgcct gctgctcggg atgcattggc cacgcgatct
    27361 ggtggtagct acgttgattt cgtgggcgct ggtggcggtg gcaacgtggc ttgcgcaacg
    27421 aatttgtggg ccattaacac cacctgcgga agaaaatcgc gaaattgcac aacgagaaca
    27481 agaaagttaa cgctggttga ttttccgaat ttaggcctta aatcatcaac aatgcgcgtg
    27541 gatgccattt cgcagacggc gcgaaaatgg tactttaaag ggctattgcg gtaagttgac
    27601 cataatttat tcgctctaac cacataacgg gaagtaatgt gaaatattta ctcattttct
    27661 tactggtgtt agcgatcttc gtgatttcgg tcacgttggg tgcgcagaac gatcaacagg
    27721 tgacgtttaa ttatctgtta gcgcaagggg agtaccgtat ttccacattg ctggcggtat
    27781 tgtttgctgc ggggtttgct atcggttggt tgatttgtgg cctgttctgg ctgcgagttc
    27841 gtgtttccct ggcgcgcgct gaacgtaaaa taaagcgact ggaaaaccag ctttcacccg
    27901 cgactgacgt ggctgtagtg ccgcactcgt cagcggcgaa ggaataactt tctatgctgg
    27961 agttgttgtt tctgcttttg cctgtagccg ctgcctatgg ctggtatatg ggccgcagaa
    28021 gtgcgcaaca aaacaagcaa gatgaagcca accgcttgtc gcgtgattac gtagcggggg
    28081 ttaacttcct gcttagtaat caacaggata aagcggtaga cctgtttctc gatatgctta
    28141 aagaggatac aggcaccgtt gaagcccacc ttacgctcgg aaacctgttc cgttcgcgtg
    28201 gcgaagttga tcgcgctatt cgcatccatc agaccctaat ggaaagcgcc tcgctgacct
    28261 atgaacagcg tctgttggcg attcaacaac tggggcgtga ttacatggcc gccgggttat
    28321 acgaccgcgc ggaagacatg ttcaatcagc tgaccgatga aactgacttc cgcattggcg
    28381 cgctgcaaca gttgctacaa atctaccagg ctaccagtga gtggcagaaa gcaattgatg
    28441 ttgccgaacg cctggtgaag ctgggtaaag ataaacagcg cgtcgaaatt gcccatttct
    28501 actgtgagtt agccctgcag catatggcca gcgacgatct cgatcgtgcc atgaccttgc
    28561 taaaaaaagg ggcggcggca gataaaaaca gcgcccgcgt atccatcatg atgggacgcg
    28621 tgtttatggc gaaaggagaa tacgccaaag ccgtcgaaag tttgcaacgc gtcatatccc
    28681 aggacagaga actggtcagc gaaacgctgg aaatgttgca aacctgctat cagcagttgg
    28741 gtaaaactgc cgaatgggca gaattcctgc aacgcgcggt ggaagagaac accggtgccg
    28801 atgctgaatt gatgcttgct gatatcatcg aagcgcgcga cggtagtgag gctgcacagg
    28861 tctatattac acgccagctt cagcgtcatc cgaccatgcg tgtattccat aagctaatgg
    28921 attaccactt aaatgaagcg gaagaagggc gcgccaaaga gagtctgatg gtgctgcgtg
    28981 acatggttgg cgagaaggtg cgtagtaagc ctcgttatag ctgccagaaa tgtggtttta
    29041 ccgcatacac cctctactgg cattgtccgt cttgtcgtgc ctggtcaact attaaaccga
    29101 ttcgcggtct tgatggcctg taatttttaa aaaaatccga ctttagttac aacatactag
    29161 ttattgatgt cccattgtgc tccggcaacg acggagcaga aaaagcctgc taggggaaaa
    29221 atcgcaactg ttaatttttt attttcacgg gtagaatgct cgccgtttac ctgtttcgcg
    29281 ccactcccgg tgcccatcat caagaaggtc tggtcatgac gttaactgct tcatcttctt
    29341 cccgcgccgt tacgaattct cctgtggttg ttgcccttga ttatcataat tgtgatgacg
    29401 cgctgtcctt tgtcgacaag atcgacccac gcgattgtcg tctgaaggtc ggtaaagaga
    29461 tgtttacatt gtttgggcca cagtttgtgc gcgaacttca acagcgtggt tttgatatct
    29521 ttcttgacct gaaattccac gatatcccca acactgcagc gcacgctgtc gctgctgcag
    29581 ctgacttagg cgtgtggatg gtgaatgttc atgcctccgg tggggcgcgt atgatgaccg
    29641 cagcgcgtga ggcactggtt ccgtttggca aagatgcacc gcttttgatt gctgtgacag
    29701 tgttgaccag catggaagcc agcgacctgg ccgatcttgg cgtgacactg tcacctgcag
    29761 attatgcaga acgtctggca gcactgacgc aaaaatgtga ccttgatggt gtggtgtgtt
    29821 ctgctcagga agctgtgcgc tttaaacagg tattcggtca ggagttcaaa ctggttacac
    29881 cgggcattcg tccgcagggg agtgaagctg gtgaccagcg ccgcattatg acgccagaac
    29941 aggcgttggc ggctggtgtt gattatatgg tgattggtcg cccggtaacg caatcggtag
    30001 atccagcgca gacactgaaa gcgatcaacg cctctttaca gcggagtgca tgatgagtga
    30061 ttccaacagc cgtctggtct actcaacgga aaccgggcgt attgatgagc ccaaagcggc
    30121 cccagtacgc cctaaaggcg atggagcggt gcgtattcag cgtcagacca gtggacgtaa
    30181 aggtaagggc gtttgcctga ttaccggtat cgatctcgat gatgccgaac tgacaaaact
    30241 tgcagcggaa ctgaagaaaa aatgcggctg cggcggagca gttaaagatg gagttattga
    30301 aatccagggc gataagcgtg atttattaaa atcactactt gaagcgaaag ggatgaaggt
    30361 aaaactcgca ggcggttaac ataaaaagcc acggatatat ccgtggcttt cgaatatttt
    30421 actgtgtgta ttattcagtt atttcgtact gaacagaatc aagcgtatta taattgcgag
    30481 tcgataatcg acatttattt acgattattt accgacctgg tgaccaataa caccaccgac
    30541 agctgcacca cctaatgtac ccaacgtact gccatcggtc agtactgcac cgcctaatgc
    30601 ccctgcaccc gcgccgattg cggtgttgcg gtcccgttta gaccagttag aacaggcact
    30661 cagagacatt gccaaagtaa ttgccagaac agccgcggtc atttttttgc tcgttacaaa
    30721 cataatactc tctcctgaat ttatgattca cggaagtaag ctctctataa ctataatagc
    30781 taagaataag cctggtgaaa ttattccgtg aaatctgctc gcgcgcaggg ttatatcacg
    30841 caggtgataa ttacttcctg ttatatcgct gataataatt ttatatcttg agagtgttaa
    30901 taacaggtaa atagtcttaa ttatcaacca ggaatcatct tagagcggat gatttgccaa
    30961 actgcaaatc atccgtagag aagggaaatg gttaaatgtc aacgacgtga acggtgagtt
    31021 tactgtgctc aagatgcatc agatcgctgg cgcgtatttt cgaatcggta atcacgcgat
    31081 tgaatcgctc aacgggaccg atggagtatg aatgtacagc accaaatttc gagctgtcag
    31141 tcaggactat cgcttcgcac tctttttcca gcacggcatt gaccacatcg gtgcgcatca
    31201 tatcgcgacc ggtgaatcca gtttcaggtt gccagccatc aataccaata aatgctttgc
    31261 tgaaatgtac ctgctggatg cactggcgtg tcaaagggcc aaccatactt tcgctttttt
    31321 tctggtacac gccaccgagc aaaatgactt cacaaggcgc gtctttcagc aaatgcgcga
    31381 tgtagctgct gaccgtgatg atagtgacat ttttcttctg ctcccccaga gtccgggcga
    31441 gcagggcatt gctgctgcca ttttcgataa agatggtttc gcccggttga accagtgacg
    31501 cggcaaactc ggcgagttca cgcttcagcg tatagttgct catcatacgg gtttcgacgt
    31561 catcactatc aagcgaaact gcaaagccat gtgcacggcg gaggtaactc agtttttcga
    31621 gggtgttgag atcctggcga atggtaactt cagaaactcc agtggctttt gccagatcgg
    31681 ttacgctaac ctgaccctgg tcaatgacca tctgtagaat agtttgttgt cgggaattca
    31741 tcgctgtaat tcttataacg ttataatact aaataaaaaa atactgacgc gccgggcatc
    31801 ctcaaatagg aaaacctgaa ggcacgattg ttaaatctcc cacggtgtct tcgggatgtc
    31861 tggcgtagat tcagcaacct ggcgcaagag ttctgcttta agtatctcga gattatggat
    31921 cgcagagtgg taatcgcctg ccacaaggat atctaacaca atatcaatac gttcggcaac
    31981 gcgctgggca tcaaattcag acatcaagga catccttata gcaaaagggg gaaaatgaat
    32041 aatgcaaaaa ttgccgctaa aagagaagtg tttaacagca acggctaatt atcatccagg
    32101 aatacgatat ataatgacgg gatatagcgc taagtatata tattcatcta cttatgcgcg
    32161 cttcagatag cgtttatacc agcgttcgaa ggcgacggcg ggcatcggtt tggcaaacaa
    32221 aaatccttgc cgctcattga tcccgttctt ggttaaaaaa gcatcttcct ttgcactctc
    32281 tacaccttcg gcgatcacct gaagattcaa tgcctgggcc acagcgacga tcgcccggac
    32341 cagtgactgc gagacaggtt gtttgtgaat atctcgaaca aaaacctggt caagtttgat
    32401 ggcatcgatc ggaaagcgcg ccagttgcga aagtgaagag tagccggtac caaaatcatc
    32461 cagatgcact tgcgcaccta gttggctaaa ttgttgaata acagacagtg ccagttcatc
    32521 attctcaatc agacaactct ctgtcagttc aacatctata gggcagtatt caaaattgag
    32581 ttcctggaga acctgtttca gggcggtgaa aatggtttga tcggcgagct gacgtgcaga
    32641 aatatttacc gccactcgca ggtttatgcc tttatcccgc cactttgcca cctggcgtac
    32701 gacatcgaga atcacccaac ggcctaaagg cacaattagc cctgactctt cggcgtagga
    32761 aatgaagtcc aacggtggaa tcaacccacg ttcaggtgac tgccaacgta ctagtgcttc
    32821 cagactgcgc acttcgacac gctaggtgat tttcgataac caactgatcg ttttccagtg
    32881 ctttacgcaa gttggtatcc agccagagat attcaaatac ccgctgattc atttctggag
    32941 taaaaacgca aaattgtcct cgtccgcctt ccttcgctgt gtacattgct gtgtcggcgt
    33001 gacgaataat agtcgcgctg tccgaaccat gttcgggaga gagcgaaata cctactgaac
    33061 agctggtata aacttcaatt aaaccaatac gaaaggggag gcgtaagcgg gtcaaaattc
    33121 gtgatgccat tgcttccagc gcgctttgtg aggtattgga tgccagtacc agaaactcat
    33181 cgccacctgg acgcgccaac acctggtcat gttcgagaca gcttaaaata gccaatgaca
    33241 cgtcgcgtaa taactggtca ccaaacaaat gcccataggc gtcgttgacc tttttaaaat
    33301 tatccaaatc aagatacaca accccaactt tattgttatc tgcctgatta atagcgtgat
    33361 cgattaactc ctgcattgcg ttacgattcg gcagtccggt gatactgtcg gtatttgcca
    33421 gaatacgcag tcgctcctga gtgcggcgct cttcggtaat gtcggtaccg gaacagatta
    33481 aaaaaatctc gtttttgcca ctgccgctgt ggacaaattt attgcgaaac agaaacagcc
    33541 gctggccttt acgtgttggt atccacagtt cgacttcata tgcattgccg cttcgaaaaa
    33601 atacacggtt attgcgcctg gatgccgcag cttcacgacg gctcataaac agtttaaaca
    33661 cgctttaccc aatgacgtcg tgttctttca accctgtgta atcttcacat aaccgattga
    33721 agcgttggat attcccccgg ctgtcgagaa tcactattgc ggaatgtgct tcagaaacta
    33781 cctgctcagc aaaggaaagc ccgtgtgata aatcacgagc aaccgccggt gtgtcatgcc
    33841 atgcagacgc gttgccagcc cattcccgtt tattgatttt tcgtcctacc agatgcacag
    33901 aaagatcgct ttcatcgacg gtcagactca tcatcaggct ggaggtgatg accgtcattt
    33961 ccctgatgcg agcagcctgt tcggcagata actgcaatgt acgatcaggt tctgtggttt
    34021 tggtggtaga aaagcgcaaa gcatcgctgc tttccgtcaa ccgccagtat ggattgtgcg
    34081 atccgagaaa gttgtacaac gttgtggact ccctaacggt tttcatgatg catatcctcc
    34141 ggttaacagc ggagtccgcg gttaaataaa aggaacaaca tgacttacat gaaattaacg
    34201 gcggctaaac gcattgtttt tttatatatt tatttgtaat ccagttttgg aaaaacgcca
    34261 gttttcaaac gaaagtcagt taaaaaatct gcctggatat aacgaaggta gagcggggaa
    34321 ataaacggcc catccatgag gaatgggccg tgaaaggaga ttacgcgacc gggcgcgcaa
    34381 taatgctgcg ggtttccatg cggacttcgg caatggtgac gtcaataacg tcagttactt
    34441 tgtaaaccgt ttcaccttta atttgtacag tgccgttttc ctggctgcaa accagttcat
    34501 cgcgcacagc gtgtaagaaa ggtgccggaa taaaggcgat agcgccgtta tcaaccaaac
    34561 gcacacgcat gccgccacgg ctgatatcga caatttccgc cgcgaaacgg gtgccggtcc
    34621 cggctttatc tttcaggaag cgtgcgtata accagtcgcc aacatcacgt tctgccatcc
    34681 ggttgagacg gcgacgttcg gccatttgga cagtgatttc atcctgcgga cgcgtcgcag
    34741 tttcgccttt gataaccgct ttcagcagac ggtggttgat catgtcgcca tatttacgga
    34801 tcggcgaagt ccaggtggcg tatgcttcca gaccgaggcc aaagtgagga ccgggttcag
    34861 agctaatttc agcaaatgac tggaagcgac gaatgcggct gtcgaggaaa ccagttggtt
    34921 gcgcatccag ttcacgacgc agtttgcaga aaccgtccag cgtgagcact tcttcggcat
    34981 cgacatgcag accgtgcgtt ttcagcaacg ctgccagcgc gtcggcattc gccggatcaa
    35041 agcccatatg cacgttatag atgccaaaac cgagcttatc gcgcagtacg cgggccgcac
    35101 aaatgttagc ggcaatcatc gcttcttcga cgatacggtt ggcaatgcga cgaggctcgg
    35161 cgacgatatc cagcacttca cctttttcac cgagaataaa gcggtaatcc gggcgatctt
    35221 taaacaccag tgcgtggtta tgacgccact cgccgcggcg ttggcaaatt tgcgctagca
    35281 aacggacttg ttcggcaatt gcttcacttt caggcttcca gtcaccggta ttttccagcc
    35341 agtcagaaac ctggtcatac accagcttcg ctttggattc gatggtggcg gcaaagaatt
    35401 cgatattatc ttcaatggtg ccatcagcgg agagcgtcat gcggcatgcc agtaccgggc
    35461 ggacttcatt ggcgcgcagt gagcagagat cgtcagaaag ctcgcgaggc agcataggga
    35521 tgttgaagcc aggcagatag ttggtgaatg cgcgaatttt cgcggctttg tccagcttgc
    35581 tgccttcagc aatccacgcg gttggatcgg caatcgccac aatcagctga agtttgtcat
    35641 ccggcagcgc cttagcgaaa agggcgtcat ccatatcttc agtgctggca ctgtcgatgg
    35701 tgacaaaatc cagcgaggtc agatcttcac gaaccagacc ttcatcgagc atttcggtag
    35761 cgacgccgtc tggtgcttct ttttccagat tatggcgcgc aagggtaacc caccacggta
    35821 caaagtggtc gtcaccaaaa gtgatgtatt gtgtcagttc agcatagaaa gaacgatcgc
    35881 ctttcagcgg atgacggcgc atttcggcaa ccgcccagtc gccttcttta aactcgtggt
    35941 tcaggccacg ggctgcgcgg caaggaatgg cgtcttttaa aagtggatga tcaggaacga
    36001 tggtcagacg gtcatttttg ccctgaactt tacccacgaa acgagtcagg aacggttcaa
    36061 ccagttcttc tggctctgcg gattcacgtt ctttttcact gtggatcacc gcgataattc
    36121 ggtcgccatg catgactttt ttcatctgcg gcggcggaat gaaataactt ttttgtgcgt
    36181 cgacttccag gaagccaaag cctttttctg tggcttttac caccccttca gcgcgtggcg
    36241 tctgggaatg cagttgctgt ttaagctgcg ctagcagcgg gttgtcctga aacataattg
    36301 tctattttgg tggccattag agcggctgac agttttacgc gaatctgtct gacgcggcaa
    36361 ggttaatatg tctcacccaa cgcgattttt aagcgattta tccagccaca cagcccgctc
    36421 cataccagca gattaataat ctgcgttgat gatttttcgt gttcgagtaa agggctgaac
    36481 tgggcggcgc taaagcgatc gggtgctcgg gtcagccatt gtatcgcctg aacggtagcg
    36541 cgctcaacag aatgacggtc agcccatcgg ttaatttcat tttcgccctt gcgcagaagg
    36601 gtgctgattt ttttatattc atttgatgaa tccatacagg tggaaaaaca agcaggactg
    36661 ccatttatac gcgcgcataa caacgccgca agctgattat ccggctgtgc attgacccgt
    36721 gtgctgttta acaacttttc gagaagggaa agtgaaatag ggtggagaga gagtgtttcg
    36781 gccagttggc aaagttctgc agttgaactt tttgtgtgct cttcaggtag tttttccacc
    36841 tcatagctgc ttcgccagcg tattgattca tcagcaaaca gtgacgcgtc ggcgtagttt
    36901 tgtatcgcca gcccgggtaa ccagcgtacc ggatgcccga gataagcctg aaatgtcgca
    36961 attgtccgcg cctgaaagcc aataaaacca atgatttggt taatgatcac acagtcccag
    37021 acagtaagac cgacgtcatt cagatgctgg cgcgatcggt tgtcgataat agaaggcgag
    37081 ctagccagtt ggcgggcata ttgcgtgatt tgtgccaacc gatgattact ttccctggag
    37141 gaatcaggtc cggggagcgg cgtaagtcgc gccgaatagt agttacataa ccgctggacg
    37201 ccgcaaacct gagcaaccgt cagggccgtc gacaagcgtt cataagcggt gaaggtttgt
    37261 gaacgggtca cggtaagctg gtcagggaac aactccactg caagaacgcg cgcagggacc
    37321 agccatgaaa ggaagggggc cagcgtctct tcatcaagga tcacgtcgag cagaaaggga
    37381 tcgctgacct ttgcggcttc cgggaccaga ggcagaacgt catactccgt agtactggat
    37441 tgcgtttcat gataccagtg gcttttgccg gtgatgtggc gttgttccat gggcgctcct
    37501 tggtcgtaaa ggaaatcgtt atcctgacgc aaggcgggaa ggggagaaag acggatcggc
    37561 gataacaaat atcagaaagg tataacagag ataacgggcg gcagaacgcc gcccatcttt
    37621 accaacagaa cgattatttc agttcgagtt cgttcattgc agcgatgctg aaaccgccgt
    37681 caacgtggac tacttcaccg gagataccgg cagagagatc ggagcacagg aatgccgcag
    37741 agttacccac atcttcaata gtaactgtac ggcgaatcgg ggtaacggct tcgcaatgag
    37801 ccagcatttt gcggaagtct ttgataccgg aggccgccag agtacggatc ggaccagcag
    37861 agatggcgtt aacacgcaca ccttccggac ccatcgcgtt cgccatatag cgcacgttcg
    37921 cttccagaga cgcttttgcc agacccataa cgttgtagtt cgggatagcg cgctcagcgc
    37981 caaggtagga aagggtcagc agggcagaac ccggattcag catggagcgg caagcttttg
    38041 ccattgcaac gaagctgtag gagctgatgt cgtgggcaat tttgaagcct tcacgggtaa
    38101 cggcgttaac atagtcacca tccagctgat cgccaggtgc aaaaccaata gagtgtacga
    38161 aaccgtcaaa tttcggccaa actttcccca gttcagcgaa catggtgtcg atgctggcat
    38221 cttctgcaac atcgcactgc agaacgatgt cagaacccaa ttgagcggca aattcttcta
    38281 cgcggccttt cagtttgtcg ttctggtagg tgaatgccag ttcagctcct tcgcggtgca
    38341 tcgcctgagc gataccgtag gcgatggaca gtttgctggc aacaccggtt accagaatgc
    38401 gcttaccgga aagaaaaccc atagctttaa tccttattgt tgatgcttgt tgtgcctgaa
    38461 aatcaggcga ttcgtcgttt tagtaaacag tacgaacaga taaacggtta ttataatcaa
    38521 cctggctgtg agtagctata gttaccaggt ccgaccggag caggctgcgg cagggggggc
    38581 gcttttcccc ttaccctaac cctctcccca gaggggcgag gggaccatat tgtgcaaata
    38641 ttgttacccc agcaacaaac aggctcatac agcccctaac ccttttatgg cgatggctgg
    38701 gatggttcag accttgccga atattctcca gcactgtctc catgttttca ccacaacagc
    38761 tattcggctc ggtcggcccc ctcgcccttt cagggtgagg gtgaccggcg gttcagttcc
    38821 tgcagaataa tctccagcac cgcctcctca ttacaatcaa tctcgttatt ccagaaacgc
    38881 agcacggtcc agccctgcga ctcaagccag ctagtgcgcc tggtatcata ggcaactgcc
    38941 aaatcatgct gcccaccatc cagctcaacg actacgcgcg ccgagcagca agcaaaatcg
    39001 agaatgtagc tccccaccgg atgttgacgg cgaaatttga aatcaccaaa acgtcggctg
    39061 cgaagatatc gccagagctt tcgttcctgc aaagtgagat tgcgtcttaa atcacgcgca
    39121 tttgatttta ttttatccat cacctcattc tgacaagatt taatctcttg tcagaatgag
    39181 gtgaataatc tggaaggagg attcagaaaa ttagcgatct ttacgccacg catccgctgt
    39241 caatgcttcg ccaaagtgcc cggcaatcag ccgtttggtg agttcatgca gcggcgatgc
    39301 cagcacatcc gcggtgctgc ctcgctcgac aacctcgccc tgatgcatca ccagcacctg
    39361 gtcgctaatg tgcttcatca ttccgatatg ctgggtaaca taaatatacg aaatgccctg
    39421 tttttcctgt aactccagca tcagattaat caactgcgaa cgcattgaca tatccagtga
    39481 agcgagggct tcatcggcaa taatgacttt tgggcgcaat atcagcgcgc gcgccagacc
    39541 cagacgctgt ttttgtccgg gtgccaacat atgcggatag taactgacgt gatccggtag
    39601 cagcccaacc atacgcatcg tttcaataat ctgtttgcga cgctgttccg gttccaggtc
    39661 ggtgttcagg cgcagtggaa aatccagaat ttgtgagata cgttgacggg gattcaacga
    39721 ggtcgaagga tcctgaaaaa tcatgcgaat acgctgacta cggaaggaat aatcgccaaa
    39781 atgcagcgga tgatcgtcaa tcaataactc gccgctggta ggttctatca ttcccgccag
    39841 catttttgcc agtgtggatt tacccgaacc attctcgcca ataatcgcca gtgtctggcc
    39901 ttcacgtagc gtaaagctca agggttttac cgcttctacg gtctgacgac gaaaccaacc
    39961 ggtccggtag cggaacgttt tacttaaatt acgcacttca agcagcgttt cgatcatctc
    40021 actctttctc catgttcagc gggaaatgac aggcatagag atgatttttc gcccccgtca
    40081 aacgtggcgt cacaatgcat tctcgttgtg catacgggca acgtggcccc agacgacaac
    40141 caatcggtaa ctgttccagc aatgggatag cgccgggtag agtattgagg cgacttttat
    40201 gcggcatcgc gctgccgaag tctggtatcg cgcggatcag cgcctgggta taaggatgat
    40261 gtggcatcgt caccaactcc ttactcggcg cggtttccac tgtttgaccg cagtaaagca
    40321 cgttaatttt atccgcccat tggctaagca tttgtaagtc atggctgata agcaaaatag
    40381 tggtattgct gttttggttg agacgcgtca gcaggcgaaa gatttgcgcc tgggttgttg
    40441 gctccattga gtttgtcggt tcgtcagcaa tcagcagacg cggttgattc gccagtgcaa
    40501 tggctatcat cactttctga cattcacctt cggtcaactc atagggaaaa ctgcgcatcg
    40561 catctttgtg atctttaatc cccacgcggt gcagcagttc aatggcacgg cgtttgcgcc
    40621 agccaaaacg ctgccaccaa cggcctttat aggtccaggc tgggatgttt tgcatcaact
    40681 ggcgacccac acgttctgaa gggtcaagac acgactgcgg ttcctggaaa atcatcgaca
    40741 cgttatggcc aaccagtttg cgccgttcgc gtgcggagag acgcagcaaa tcgatatcat
    40801 caaaacgcat acggtcagca gtaacacgcc agttatcttt attcacccca caaatcgctt
    40861 tcgcaatcaa actcttgccg gaaccggatt caccaacaag accgcggatt tcaccttcgg
    40921 ttaaggtcat acttacccgg tcgacggctt taacccactc atcaccggtt ttaaattcaa
    40981 tggtcaggtt acgaatatcg agtaatggca ttattccacc cccgcaataa tcgcacgacg
    41041 aacgccatcg ccaaggaggt taaccaacaa cacgctaatc ataattgccg cacctggcag
    41101 catgacagtc cacggggcga cataaatcag ttccagcgca tcaccgagca tcgctcccca
    41161 ttcaggcgag gggagttgtg cgccgagatc gagaaagccc agcgcggcga tatcgagaat
    41221 tgccatcgac agtgcgcggg tgatctcggt gaccagcccg gcggtgatgt ttggcatcac
    41281 agcaaaccag agaatattca gcgttgatgc gccatccaga cgggcggcga taacgtactc
    41341 tttttccagt tcgtcatgca ccatgctgta aatcgaacgt accatacgcg gcagcaatgc
    41401 cagccagaca gcaaacatag cgtgggacaa actcggtccg gcaaacgcta caacgataat
    41461 tgccaacagc agcgaaggga tcgccagtaa ggtatccaga atatggttaa gcaccgctga
    41521 gcggaggccg tgtgtcgccc cggcgaaggt gcccagtacc aggccacaga tcgtcgcggc
    41581 aagcgtcacc acaaatgcgc cacccacggt tggcgcagct ccgctcagta aacggcttaa
    41641 aacatcacgt ccgaggtcgt cagtccccag gaagaaagaa acttcgccat agcgcgacca
    41701 tgatggcggc aataattgat aaccgagaaa ttgctggtcg atgccgtaag gcgcaaacca
    41761 gccgccaaaa atacacagta cagccagtcc cgcgcagccg tacaggccga ccatcgcaga
    41821 ggcgtcacta taaaatttgc gccaggcggt acgcagcgtg cccggcgggc gcttttcgct
    41881 gtatacgcta tcgtaaggca taccattcct tatgtttcag agggttagcc atggcaccca
    41941 aaatatcaga aatcacgtta acaataatga ccagtgagcc acacaccatc actccggcgg
    42001 aaatggctgc gtaatcctgc tggcggatgg cgttaattaa ccagcgccct aaacccggcc
    42061 agctaaaaac catttcggtg atcatcgcca gcgtcagcat ggtggaaaac tgtaagccca
    42121 ggcgaggaat aaccggcggt aacgcgttat gcagaacgtg gcgacgcaaa atagtaaagc
    42181 gcgacaaacc gcgggtcgcc gccgctttta catagttctg gtcatacact tcaatagtgc
    42241 tgatacgcat caggcggatc acttcagttg ttggcgcaac cgacagggtg atcacgggca
    42301 atatcatatg gcggattgcg ctcatgatca tttcatcccg ccatggtgag tccgaaagcc
    42361 aggcatcaat caacgcaaaa ccggtaatcg gtttcacttc gtagagcaga tcgaaacgcc
    42421 ctgaaacggg caaccaacct agcgtgagtg aacaaaacag tgtcaacaga agcgccagcc
    42481 agaacaccgg gattgaaaag cccagtaagg cgatggcgtt gatcaagtta tcctgccact
    42541 tatggcgcgt aatcccggca atcatcccca cggggatgcc aacaatcagc gcaaaaccga
    42601 aggcgaggat gcacagttct atcgtcgccg ggaagagctc ttttaactgt tcggcaattg
    42661 gttgaccgtt aatgctggaa acaccaaaat cccagtggat caggccatta aaccagaaca
    42721 cccaggcatt ccacagtgac gcgccttgta acggcgcgtg tggagtgaaa tagctcaagc
    42781 tgaagccaac gaaggtcagc aggaacaagg tgacaatcaa taacaaaatg cggcgtaagg
    42841 tgaagataat catggttttt tcacctcatc ctgtttctcg cgatacaccc cagcaaagga
    42901 ggcgttacca aacgggctaa gtaccagacc tttgatatcg taccgatagg cctgcaaacg
    42961 caatgacgac gccagcggca gaataggtaa ttcctgcgcc agaatactct gcgcttcgtc
    43021 ataggcttca atacgcgctg ccagctgctg cgaggagagc gccttacgca acacgctgtc
    43081 gaatttcgga ttgcaccagt gggcgaggtt ggtctgtgaa tgaattgccg cgcagctcag
    43141 taacggacgg aagaaactgt ccgggtcgtt actgtccgtc gcccaaccgg ataacgtcag
    43201 atcatggctc atatccatca accgcgcctc ctgaaagcga ccttctaccg gcacaatcac
    43261 cacttttacg ccaacctgcg ccatatccgc ctgaatcagt tcggcagttt tcagtggact
    43321 ggggttccac gcctgcgaac gtgtgggcac ccacagtttc agcgttaagt tttccagccc
    43381 caacgccttc aactgttcgc gcgatttcgc cggattgtat tcagtaattt tagcctcgtt
    43441 gtcataggcc cacgaggcgc gcggtaaaat agaggccgcc gtttcagccg taccataata
    43501 gatggattgc atcaggcgct ggttattaat cgccagtgcc agtgcatggc ggacagcggg
    43561 attatttagc ggcggtttgg cggtgttaaa tgccagatag gcgacgttca tcccaggacg
    43621 cagcgttaaa cgcaagcgcg ggtcgtcacg caaaatggat agctggctgg cagcaggcca
    43681 ggccagaacg tcgcattccc cggtcaggag tttcgacaga cgtccggtgc cgccggagcc
    43741 taaatccacc actacctgcg gcattaacgg tttaccgcgc cagaagtcat catgacgttg
    43801 taggcgaata aattgcccgg cgcggtattc cgacaactga tacggaccgg tgccgaccgg
    43861 ttgacggtcg agttgctcct ggcgatcttc tttctctaac ttccgggcat attctgccga
    43921 catgaccgaa gcataatggg ttgcgaggtg ccacaaaaaa gaagcatccg gctgggcgag
    43981 tcggaactca acggtatgat tatccagttt gcggacgctt ttcacgttat cggcaaattg
    44041 caggctgtcg aagtagggga agttgctgcc gttgacgtta tgccacgggt tgttgcggtc
    44101 aaaaattcgc tggaaggtaa acaccacatc gtcggcattc attttacgag tgggagtaaa
    44161 ccagtcggtt ttttgaaacg gaacatcgcg acgcaggtgg aagcgatagg tcgcgccgtt
    44221 gtcgagtact tcccagcttt cggcaagttc cggcatcagg cgataggtat agggatcgac
    44281 atccagcagt cgatcataaa actgggcggc aagggtatcg acaattaacc cactgctcgc
    44341 tttggatggg ttaaaggtgt tgacttgccc gctgacgcaa tagacaaaac cgctgtcgcg
    44401 gatatcagca tgcggggggg attcaggcgc ggcgattgcc tgaccactca caagtccagc
    44461 aatcaccaaa agagacgata atacctggcg cataatatta agggatttta tgtaaagagg
    44521 ctatcttact aatatttaat gacatttgcc attaccgttt gtgttcaggg gtcgtaatga
    44581 gggccgtgtg gttggtcgtt gaacaggtgg cgtcgccatc tgttcaacat tcgtacccgt
    44641 gatttctcta taactataac tcacagaaca acttagcgag gagcagagcg gtacgatcgt
    44701 gagaaaccca ttaaagcacg ctgcagtgcg tgtcattgtt agccggatgc ggcgtgaacg
    44761 ctttatccgg acaacgatac tgaccgatcg tctgcaatta aataattact cattacccca
    44821 ttgattcaaa aactctgcga tctcatcaat gcgtacggga ttaatccccg cttcagcagc
    44881 catttcatgt tgggcttctt cgctgatctc ttcattgttc atcaaacggg tgagcagtaa
    44941 ctggaagtag tgggccagcg gtgtgcgttc agcctctgcg acaggctctg cgcattcggt
    45001 cgcgtactca tcggcaatat caaaatattt tagcgggata tcgtggttca ttatttgccc
    45061 ctggggttaa tgcgctaagc ataagggcgg atgatagcac tcgtgatccg ggtcatacat
    45121 ctttttatag ttaatggcgc ggcgcgttac cctcaggcag gataatgcgc cgcgcatccg
    45181 gctattacgt ttcgctcacc ggcgttctgt caccttcgta cagcggcact ttgcgatagc
    45241 ggcggatcag ataccacaaa tatgcgccgc ctaacgaagc ccataccaga ccgagtgtca
    45301 gtgacgttga ctctaggtta acccacagca cacccaccgt cagcgcgcca accagcggca
    45361 tcagcaaata gtggaagtga tccttccagc ttttattcat tcctttacgc cgccagaaat
    45421 gattaaacac cgacagattc acgaaggtaa acgccaccag agcaccgaag ttaatcagtg
    45481 ctgtcgcggt gactaaatca aagaacagcg ccgacagcgc gacaatcccg accataatga
    45541 cgttgagtgc cggagtccgc cattttgggt gcacatagcc aaacacgcgc tccggaaaca
    45601 cattgtcacg ccccatcaca tacagcagac gcgacacgct ggcatgtgaa gccaggccag
    45661 acgctaatgt gttcacaaac gtggtgcaga ggaaaatcga ctgaaagagc ttgccgccga
    45721 cgtacagcgc aatttcaggc agtgcggcgt ccgggtcttt aaagcggctg atatcgggaa
    45781 agaacagctg cataaagaac gacgccgcga taaagataac gccgccgtat accgccgtca
    45841 ggaagatggc tttcgggatc acgcgtgcgg cgtccggcgt ctcttccgaa agcgtggtca
    45901 ccgcatcgaa accgaggaac gagaaacaga caatcgtcgc accggtaata atcgggatca
    45961 ggtgcgcgtt ctcgctgata aacggctgaa gtgaccagac ggtgccaacg ccttctcctt
    46021 tatgcagtcc ctgaaccacc aggaagataa acaccaccat gatggagatt tgcaccagta
    46081 caaacagggt attgaagtta gcgaccaggt tgacgctctt cagattcgcg gcggttaaaa
    46141 tggcgacgaa ggttaccacc cacacccacg gcggcacttc cgggaagagg gcggagagat
    46201 agattttcgc caacaaaacg ttgatcattg gcaaaaagag ataatccagc agtgatgacc
    46261 agccgaccat aaatccgacg tgcgggttaa tcgacttttg cgcgtaggta taggccgaac
    46321 cggcctccgg aaactggcga accagtttgc cgtagctgat agcggtaaac agcacgcccg
    46381 ccagcgccag caaataggac gccggaacgt gaccgtcgct aatgccggag acaataccaa
    46441 aagtatcaaa tacggtcatc ggcgtgagat aggccagacc catcattacc acctgccaca
    46501 atttcagtga ttttcgcaga cgggttttgc cgggttgcgc agcaatattc agtggtgaat
    46561 taatagccat aattgctgcc tcccctgcgt aataaggttt gcggtcgtgt cgacctgaaa
    46621 tcaaacctgt ttagcaaggg aagcaaaggg ggcggagtcc agtcagtagc aggaggggcg
    46681 catacgcagc ctccctcgcc atgcggggat ttaatcatcg gcatcatctc atttcctcgt
    46741 cacagtctta atcgttaacg gaagcccgtt gccgcctgcg ctccataacg cgacatccgg
    46801 gaaatgtctg gattaccagc aaatgtcagg cctggctccg ctcaggccga tgaaacaacc
    46861 ccgcaagggg aattacgcgt ttttcaacat ccactcaatt tctgtttctg tgatgaggcg
    46921 ctcaaactgc aacagctcat catttttaca ggcgtgatac acatggcaaa accgttcgcc
    46981 taaatagcgg cgcaggtgat cattctcgat aaactcaccc agggcatcgc tctggcgaat
    47041 cgggaagggt aagccttcct gttccagccc gttgccttcg acttcttcct gcaacggcag
    47101 ctcgttatca aggccatgca aaataccggc aaaaatcgct gccatcacca gatatgggtt
    47161 ggcatcggca cccgccacgt gatattccac gcggtgatta tgacggtcac cgcacggaat
    47221 acgcagggcg acggtgcggt tgttatggcc ccacgacgcc tgcgtcggca catacattcc
    47281 cggctggaag cggcgatacg agttcacgtt tggtgccagc aacgccatcg acgacggcat
    47341 caggtcaatc atcccggcga gcatcttttt cagcagcggc gaatcttcgc cttctgtgtc
    47401 agaaagcaca ttctcgccac gattattttg catactgata tggatatgca tcccgctgcc
    47461 cgcgtgctct tcatacggct tcgccataaa agtggcgtgc atcttatgct tttctgccat
    47521 cagacgcact aggcgtttta gtgccagcgc atcatcgcag gcttccagca cgttatcagt
    47581 atggtaaagg ttgatttcaa actgacccgg cgaggcctca gcgaccgcgc catctgccgg
    47641 aatcagctgc aactgcgcca gttcatcaat atcattgagc acgtcggcga agtggttgag
    47701 gttatcaacg gagtaaacct ggctttgtgt attgcggtca tcggtgccag gcgcgcaggg
    47761 cggttgcaga tccccttcag cgtcgcgctg gcgatccagt aaatagaact ccagctctac
    47821 cgctacaacc gggaacaatc cgcgctggcg cagctgctgc cacaggcggt taagaacgtt
    47881 ccgcggctca acgtcaaagg gagcgccatc ttcatcgacc atggtcagga gcatctgacc
    47941 gataaactct ggatcggcgg cagaaggagt taaggaaccg agaacaggaa cacaggtacg
    48001 atccggctcg cccatttcct gacccagacc tgcttcttct actacgttgc ccagaatatc
    48061 cattgcaaat accgaggccg ggaaataaca ccctttctcg agcttcttca ggcttgaaac
    48121 agggatacgt tttccacgga agcaaccatt caaatcagta agtaaaacat caacatattg
    48181 cgtattcggg tagcgctcca ggtagcgttt cacttcctgc gtaaaggcgc taccccgcct
    48241 ctcttctgac tgctgaacaa agttctctac ttcaacgata ttggtttcca tgattcttcg
    48301 cctttggttt gttttccgct cgttatcaaa gcgtaaaata taatgaccat cattcgaatc
    48361 tgtatgcaaa ccaaatgttt gtcaaatgtt aaattgagtt tgcaaaaatg aaaatccact
    48421 gctagattga aaaaatattg aacataaagg tcatttaaag cgcagtaatg gcgataattt
    48481 agtccatttt gtgagattga gcatggaaaa tataatgaac aatccggtta tcggtgtcgt
    48541 aatgtgcagg aacaggctta agggtcatgc gacccagact ctgcaagaaa agtacctgaa
    48601 tgccatcatc cacgcaggcg gcttgccaat tgcgctacca catgcgctgg cggaaccgtc
    48661 gttacttgaa caacttttgc cgaaactcga tggcatttat cttcctagta gtcccagcaa
    48721 tgtgcagccg cacctatatg gtgaaaacgg cgatgagcct gacgccgatc ccgggcgtga
    48781 tcttctgagc atggcgataa ttaatgccgc actcgaaagg cgcatcccca ttttcgccat
    48841 ctgccggggt ttacaagaac tggtggtggc aaccggtggt tcgttgcatc gcaagctgtg
    48901 cgaacagcct gaattgctgg aacatcggga agatcccgaa ctgccggtgg aacagcaata
    48961 tgcaccgtcg catgaagttc aggttgaaga ggggggatta ctgtctgcgt tgttacctga
    49021 atgtagcaac ttttgggtaa actctctaca tggacaaggg gcgaaggtcg ttagcccacg
    49081 gttgcgtgtt gaagctcgct cgccggatgg cttagttgag gcggttagcg tcatcaatca
    49141 tccttttgcg ctgggcgtac agtggcaccc ggaatggaac agtagcgagt acgcgctttc
    49201 gcgtatattg ttcgagggct ttatcaccgc ttggcagcac catatcgctg aaaaacagcg
    49261 actctgacca ctacagttta aggaaatgca aatatgagtg atgagggact ggcgccagga
    49321 aaacgcttgt cggaaatccg ccagcagcag gggctttcac aacgtcgtgc cgccgaactc
    49381 tccgggctga ctcacagtgc tatcagtacg atagaacaag ataaagtcag ccctgccatc
    49441 agtacgctgc aaaagctgct gaaggtgtat ggtctgtcac tctcggaatt cttttccgag
    49501 ccggaaaaac ctgatgagcc gcaggtcgtc attaatcagg acgacttaat tgagatgggc
    49561 agtcagggtg tgtcaatgaa gctggttcat aacggtaacc cgaatcgcac gctggcgatg
    49621 atctttgaaa cgtaccagcc gggcacaacc actggggaaa gaattaagca tcagggtgag
    49681 gaaataggca cagtactgga aggtgaaatt gttctgacga ttaatggtca ggattaccac
    49741 ctcgtcgcgg ggcaaagtta tgccattaat accggcatcc cgcacagttt cagtaatacg
    49801 tcggcaggta tttgccgaat tatcagcgcc catacgccca ccacgtttta atctttttgt
    49861 tctgtaagcc gggtaagcgc aatgcgcccg gcaatccata aagaatgtcg ccagaaataa
    49921 acgggcatgc agcccgggga tctctgcgcc ctgacgttca caaactgcat atatctgata
    49981 gacgtgaaac aggagtcata atgaattttc atcatctggc ttactggcag gataaagcgt
    50041 taagtctcgc cattgaaaac cgcttattta ttaacggtga atatactgct gcggcggaaa
    50101 atgaaacctt tgaaaccgtt gatccggtca cccaggcacc gctggcgaaa attgcccgcg
    50161 gcaagagcgt cgatatcgac cgtgcggtga gcgcagcacg cggcgtattt gaacgcggcg
    50221 actggtcact ctcttctccg gctaaacgta aagcggtact gaataaactc gccgatttaa
    50281 tggaagccca cgccgaagag ctggcactgc tggaaactct cgacaccggc aaaccgattc
    50341 gtcacagtct gcgtgatgat attcccggcg cggcgcgcgc cattcgctgg tacgccgaag
    50401 cgatcgacaa agtgtatggc gaagtggcga ccaccagtag ccatgagctg gcgatgatcg
    50461 tgcgtgaacc ggtcggcgtg attgccgcca tcgtgccgtg gaacttcccg ctgttgcaga
    50521 cttgctggaa actcggcccg gcgctggcgg cgggaaacag cgtgattcta aaaccgtctg
    50581 aaaaatcacc gctcagtgcg attcgtctcg cggggctggc gaaagaagca ggcttgccgg
    50641 atggtgtgtt gaacgtggtg acgggttttg gtcatgaagc ggggcaggcg ctttcgcgtc
    50701 ataacgatat cgatgccatc gcctttaccg gctccacgcg tacgggtaaa caactgctga
    50761 aagacgcggg tgatagcaac atgaaacgtg tctggctgga agcgggcggc aaaagcgcca
    50821 acatcgtttt cgccgattgt ccggatctgc aaaaagcggc aagcgccacc gccgcaggca
    50881 tcttctacaa ccagggccag gtgcgcatcg ccggaacgcg tttgttactg gaagagagca
    50941 tcgccgatga attcttagcc ctgttaaaac agcaggcgca aaactggcag ccgggccatc
    51001 cacttgatcc cgcaaccacc atgggcacct taatcgacag cgctcacgct gactcggtac
    51061 acacctttat tcgtgaaggc gaaggcaagg ggcaactgct gctggatggt cgcaacgccg
    51121 ggctggcagc ggccatcggc ccgaccatct tcgtggatgt ggacccgaat gcgtccttaa
    51181 gccgcgaaga gattttcggt ccggtgctgg tggtcacccg tttcacatca gaagatcagg
    51241 cgctgcagct tgccaacgac agccagtacg gccttggcgc ggcggtatgg acgcgcgacc
    51301 tctcccgcgc gcaccgcatg agccgacgcc tgaaagccgg ttccgtcttc gtcaataact
    51361 acaacgacgg cgatatgacc gtgccgtttg gcggctataa gcagagcggc aacggtcgcg
    51421 acaaatccct gcatgccctt gaaaaattca ctgaactgaa aaccatctgg ataagcctgg
    51481 aggcctgaaa tgaccgaaca taccagcagt tactacgccg ccagtgcgaa taaatatgca
    51541 ccattcgaca cactgaatga gtcaatcacc tgcgacgttt gcgtggttgg cggcggctat
    51601 accgggctct cctccgcgct gcatctggcg gaagcgggct ttgacgtagt ggttctcgaa
    51661 gcctcacgca tcggctttgg cgcaagcggg cgcaatggcg ggcagcttgt gaactcctac
    51721 agccgcgaca tcgacgtgat cgaaaaaagc tacggcatgg acaccgcccg tatgctcggc
    51781 agcatgatgt tcgagggtgg cgagatcatc cgcgaacgta tcaaacgtta tcagattgac
    51841 tgcgactacc gccccggcgg tctgtttgtg gcgatgaatg ataaacaact cgccacactc
    51901 gaagagcaga aagagaactg ggtacgctac ggcaataaac agctggagct gctggacgtc
    51961 aacgctattc gccgcgaggt cgccagcgat cgctacactg gtgcgctgct ggatcacagc
    52021 ggtgggcata ttcacccgct aaacctcgcc attggtgaag ccgatgccat ccgcctgaac
    52081 ggcgggcgcg tgtatgagct ttccgccgtg acgcagatcc agcacaccac gccagccgtg
    52141 gttcggactg ccaatggtca ggtgacggcg aagtatgtga ttgtcgccgg gaatgcgtat
    52201 ctgggcgata aagtagagcc ggaactagcg aaacgcagca tgccgtgcgg cacgcaggtg
    52261 atcaccaccg aacggctgtc ggaagattta gcccgttcgc tgatcccgaa aaactactgt
    52321 gtggaagact gtaactatct gcttgattac taccgtctta ccgccgacaa ccgcctgctg
    52381 tacggcgggg gtgtagtgta tggtgcacgc aacccggatg acgttgagcg tcttgtggta
    52441 ccgaaactac taaaaacctt ccctcagttg aagggcgtga aaattgatta ccgctggacg
    52501 ggcaacttcc tgctgacctt gtcgcacatg ccgcagttcg gccgccttga taccaacatc
    52561 tattacatgc agggttacag cggccacggc gtcacctgta cccatcttgc cgggcgtttg
    52621 attgctgaac tgctgcgcgg tgacgccgag cgtttcgatg ccttcgccaa tctgccgcat
    52681 tacccgttcc ccggcgggcg cacgctgcgc gtgccattta ccgcgatggg cgcggcttat
    52741 tacagcctgc gcgatcgtct gggcgtttaa tttccgatta acagtgaaga gtcaaaaggt
    52801 gtgaaacatg agcaacaatg aattccatca gcgtcgtctt tctgctaccc cacgcggggt
    52861 tggcgtgatg tgtaacttct tcgcccagtc ggcagaaaac gctacgctga aggacgtaga
    52921 gggcaacgaa tacatcgatt tcgccgcagg cattgcggtg ctgaataccg ggcatcgcca
    52981 ccctgatctg gtcgcggcgg tggagcagca attgcaacag tttacccaca cggcgtatca
    53041 gattgtgccg tacgaaagct acgtcacgct ggcggagaaa attaacaccc ttgccccagt
    53101 gagcgggcag gctaaaaccg cattcttcac taccggcgcg gaagcggtgg aaaacgcggt
    53161 gaaaattgct cgcgcccata ccggacgacc tggcgtgatt gcgtttagcg gtggcttcca
    53221 cggtcgtaca tatatgacca tggcgctgac cggaaaagtt gcgccgtaca aaatcggctt
    53281 cggcccgttc cctggttcgg tgtatcacgt accttatccg tcagatttac acggcatttc
    53341 aacacaggac tccctcgacg ccatcgaacg cttgtttaaa tcagacatcg aagcgaagca
    53401 ggtcgcggcc attattttcg aaccggtgca gggcgagtgc ggtttcaacg ttgcgccaaa
    53461 agagctggtt gccgccattc gccgcctgtg cgacgagcac ggtattgtga tgattgccga
    53521 tgaagtgcaa agcggctttg cgcgtaccgg taaactgttt gccatggatc attacgccga
    53581 taagccggac ttaatgacga tggcgaaaag cctcgcgggc gggatgccgc tttcgggcgt
    53641 ggtcggtaac gcgaatatt
//
LOCUS       DAJTSI010000008        53211 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000008 DAJTSI010000000
VERSION     DAJTSI010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.008"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(11..322)
                     /locus_tag="OO327_000424"
     CDS             complement(11..322)
                     /locus_tag="OO327_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCR6017096.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="OO327_000425"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO327_000425"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="OO327_000426"
     CDS             701..1054
                     /locus_tag="OO327_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR6017097.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="OO327_000427"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="OO327_000427"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCR6017098.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="OO327_000428"
     CDS             complement(2810..3280)
                     /locus_tag="OO327_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCR6017099.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="OO327_000429"
                     /pseudo
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="OO327_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            complement(4119..4337)
                     /gene="hha"
                     /locus_tag="OO327_000430"
     CDS             complement(4119..4337)
                     /gene="hha"
                     /locus_tag="OO327_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR6017100.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="OO327_000431"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="OO327_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR6017101.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5283..8432)
                     /locus_tag="OO327_000432"
     CDS             complement(5283..8432)
                     /locus_tag="OO327_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR6017102.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="OO327_000433"
     CDS             complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="OO327_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR6017103.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9790..10437
                     /gene="acrR"
                     /locus_tag="OO327_000434"
     CDS             9790..10437
                     /gene="acrR"
                     /locus_tag="OO327_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR6017104.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10565..13927
                     /gene="mscK"
                     /locus_tag="OO327_000435"
     CDS             10565..13927
                     /gene="mscK"
                     /locus_tag="OO327_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR6017105.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="OO327_000436"
     CDS             complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="OO327_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR6017106.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14314..14841)
                     /gene="priC"
                     /locus_tag="OO327_000437"
     CDS             complement(14314..14841)
                     /gene="priC"
                     /locus_tag="OO327_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR6017107.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14911..15288
                     /locus_tag="OO327_000438"
     CDS             14911..15288
                     /locus_tag="OO327_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR6017108.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15441..15992
                     /gene="apt"
                     /locus_tag="OO327_000439"
     CDS             15441..15992
                     /gene="apt"
                     /locus_tag="OO327_000439"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR6017109.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16121..18046
                     /gene="dnaX"
                     /locus_tag="OO327_000440"
     CDS             16121..18046
                     /gene="dnaX"
                     /locus_tag="OO327_000440"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR6017110.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17379..17443
                     /gene="dnaX"
                     /locus_tag="OO327_000440"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18099..18428
                     /locus_tag="OO327_000441"
     CDS             18099..18428
                     /locus_tag="OO327_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR6017111.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18428..19033
                     /gene="recR"
                     /locus_tag="OO327_000442"
     CDS             18428..19033
                     /gene="recR"
                     /locus_tag="OO327_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR6017112.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEARRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19143..21017
                     /gene="htpG"
                     /locus_tag="OO327_000443"
     CDS             19143..21017
                     /gene="htpG"
                     /locus_tag="OO327_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR6017113.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21198..21842
                     /gene="adk"
                     /locus_tag="OO327_000444"
     CDS             21198..21842
                     /gene="adk"
                     /locus_tag="OO327_000444"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR6017114.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21974..22936
                     /gene="hemH"
                     /locus_tag="OO327_000445"
     CDS             21974..22936
                     /gene="hemH"
                     /locus_tag="OO327_000445"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR6017115.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22933..23892)
                     /gene="aes"
                     /locus_tag="OO327_000446"
                     /pseudo
     CDS             complement(22933..23892)
                     /gene="aes"
                     /locus_tag="OO327_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            24044..25348
                     /gene="gsk"
                     /locus_tag="OO327_000447"
     CDS             24044..25348
                     /gene="gsk"
                     /locus_tag="OO327_000447"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR6017116.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25481..27157)
                     /locus_tag="OO327_000448"
     CDS             complement(25481..27157)
                     /locus_tag="OO327_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR6017117.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="OO327_000449"
     CDS             complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="OO327_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR6017118.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIEPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28833..30485
                     /gene="ushA"
                     /locus_tag="OO327_000450"
     CDS             28833..30485
                     /gene="ushA"
                     /locus_tag="OO327_000450"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR6017119.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="OO327_000451"
     CDS             complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="OO327_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR6017120.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31205..31999)
                     /locus_tag="OO327_000452"
     CDS             complement(31205..31999)
                     /locus_tag="OO327_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR6017121.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32137..32478
                     /locus_tag="OO327_000453"
     CDS             32137..32478
                     /locus_tag="OO327_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6017122.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32693..35197)
                     /gene="copA"
                     /locus_tag="OO327_000454"
     CDS             complement(32693..35197)
                     /gene="copA"
                     /locus_tag="OO327_000454"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR6017123.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35459..36391
                     /gene="glsA"
                     /locus_tag="OO327_000455"
     CDS             35459..36391
                     /gene="glsA"
                     /locus_tag="OO327_000455"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR6017124.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36394..37686
                     /locus_tag="OO327_000456"
     CDS             36394..37686
                     /locus_tag="OO327_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6017125.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37811..38218
                     /gene="cueR"
                     /locus_tag="OO327_000457"
     CDS             37811..38218
                     /gene="cueR"
                     /locus_tag="OO327_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR6017126.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38219..38677)
                     /locus_tag="OO327_000458"
     CDS             complement(38219..38677)
                     /locus_tag="OO327_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR6017127.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38674..39591)
                     /locus_tag="OO327_000459"
     CDS             complement(38674..39591)
                     /locus_tag="OO327_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR6017128.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39737..40414
                     /gene="fetA"
                     /locus_tag="OO327_000460"
     CDS             39737..40414
                     /gene="fetA"
                     /locus_tag="OO327_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR6017129.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40401..41180
                     /gene="fetB"
                     /locus_tag="OO327_000461"
     CDS             40401..41180
                     /gene="fetB"
                     /locus_tag="OO327_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR6017130.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="OO327_000462"
     CDS             complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="OO327_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR6017131.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="OO327_000463"
     CDS             complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="OO327_000463"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR6017132.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="OO327_000464"
                     /pseudo
     CDS             complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="OO327_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43550..44236
                     /locus_tag="OO327_000465"
     CDS             43550..44236
                     /locus_tag="OO327_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6017133.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44233..46648
                     /locus_tag="OO327_000466"
                     /pseudo
     CDS             44233..46648
                     /locus_tag="OO327_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(47361..48455)
                     /gene="mnmH"
                     /locus_tag="OO327_000467"
     CDS             complement(47361..48455)
                     /gene="mnmH"
                     /locus_tag="OO327_000467"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR6017134.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLYEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48524..49450)
                     /gene="allS"
                     /locus_tag="OO327_000468"
     CDS             complement(48524..49450)
                     /gene="allS"
                     /locus_tag="OO327_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR6017135.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49680..50162
                     /gene="allA"
                     /locus_tag="OO327_000469"
     CDS             49680..50162
                     /gene="allA"
                     /locus_tag="OO327_000469"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCR6017136.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGIQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50240..51055
                     /gene="allR"
                     /locus_tag="OO327_000470"
     CDS             50240..51055
                     /gene="allR"
                     /locus_tag="OO327_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCR6017137.1"
                     /translation="MTEVRRRGRPGQAAPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51145..52926
                     /gene="gcl"
                     /locus_tag="OO327_000471"
     CDS             51145..52926
                     /gene="gcl"
                     /locus_tag="OO327_000471"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCR6017138.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52939..>53211
                     /locus_tag="OO327_000472"
     CDS             52939..>53211
                     /locus_tag="OO327_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCR6017139.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        12831 a        13691 c        14015 g        12674 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca atgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtgcc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgat tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccagg aagggcagta tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gaactatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctagc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgcggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aattagcgag aatttactgg cgtaatgtgt gctcttccgc cagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagca ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcgt tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc caaccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctagacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt
     5101 attaaatcta ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt
     5161 ttatacgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct
     5221 taatgttcgt aggttatgca taaaaaaggc cgcttacgcg gccttagtga ttacatgttg
     5281 tatcaatgat gatcgacagt atggctgtgc tcgatatctt cattcttgcg gctaaagcgg
     5341 cggcgaacca ccacaaagaa taccggaacg aagaagattg ccagtaccgt tgcggtcacc
     5401 atcccgccca ttacaccggt acctactgcg ttctgcgcgc cggaaccagc accagtactg
     5461 ataaccagcg gcataacgcc gaggataaac gccagcgagg tcatcaggat cggacgtaaa
     5521 cgcatccgca ccgcatcaag cgtcgcttca atcagacctt taccttcttt atccatcaag
     5581 tctttggcga attcgacgat aagtatcgcg ttcttcgccg acaacccaat ggttgtgagc
     5641 agtcctactt ggaagtaaac gtcattggtc aggccacgga aggtggcagc cagcaacgca
     5701 ccgataaccc ccagcggaac gaccagcata acggagaacg gagtcgacca gctctcgtac
     5761 agcgccgcca gacacaggaa tacgacaatc aacgaaatcg cgtacagtga aggtgcctgg
     5821 ttgccggaga gacgttcctg ataggacatc cccgtccagt catagccaac accggtaggc
     5881 agtttgctcg ccagttgttc catcagctcc attgcttcac cggtactttt acccggtgcc
     5941 gcctggccta agatttccat ggatggcagg ccgttgtaac gttccagacg cggcgaaccg
     6001 tactcccaac gagaagagga gaacgccgag aatggcacca tctgaccatc agcagcacga
     6061 acataccagt cgccgatatc atccggcagc atacggtatt tcgcttctga catgacgtaa
     6121 actttcttca cacgaccgcg gtcgataaag tcgttcacat agctaccgcc ccatgcagcg
     6181 cccagagtgg tgttaatgtc gttgatagaa acacccagcg cctgcgcttt ttcctggtcg
     6241 atatcaatct taaactgcgg ggtatcttcc agaccgtttg gacgtacgct ggtcagcata
     6301 tcagggtgct tcgctgcttc tgcaagcagc tggttacgcg cctgagtcag tttttcgtga
     6361 ccaaggccag cctggtcaat cagctcaaag tcaaagccgg ttgcagtacc cagttccacg
     6421 attgcgggca ggttaaaggc gaaaaccatc gcatctttga tttgcgagaa agcgcgtgtt
     6481 gcacgcatgg taatcgcttc aactttgttt tcttcgcccg gacgatcggc ccagtccttc
     6541 aaggaaacga acgcaatacc ggtattctga ccacgtcccg caaagccgaa gccgttaacg
     6601 gcgaacaccg actcaacgtt gttcttttct ttggtcagat agtaatgcgt tacctcattg
     6661 agcactttct gcgtacgttc ctgcgttgca cctgctggca gctgaaccat ggtcataaat
     6721 acgccctggt cctcatctgg caagaaggag cttggcagac gcacgaacag ataggccatg
     6781 ccgaccacga tgatcagata cagcactagg taacgccccg tactgcgcag aataccgcct
     6841 acgctgtcgg tgtagtggtg cgtgctcttc tcgaacatgc ggttcaacca gccgaagaag
     6901 ccttttttac cttccccgtg atcgcctttg gcaatcggtt tcagcatggt ggcacaaaga
     6961 gccggagtca ggatcaacgc taccagtacc gacagcgcca ttgctgaaac aatggtaata
     7021 gagaactgac gatagatagc accagtagaa ccgccaaaga aggccatcgg tacgaatacc
     7081 gccgacagta ccatcgcgat accgaccaga gcgccctgaa tctgccccat cgacttacgg
     7141 gtagcttctt ttggcggcaa accttcttcc gccataacac gctccacgtt ttctaccaca
     7201 acgatagcgt catccaccaa caagccgatg gcgagcacca tcccgaacat tgttagcgtg
     7261 tttatcgaga agccaaaggc ggcaaggacg gcaaaggtcc cgagcaatac caccggtacg
     7321 gcaatggtcg gaatcaacgt cgcgcggaag ttctgcagga acagatacat aaccaggaac
     7381 acgaggatga tcgcttcgac cagcgtttta accacttcgt gaatagagat tttcacgaac
     7441 ggcgtggtgt cgtacgggta aacaattttc agacccgacg ggaagaacgg ttccatcttc
     7501 gccagttcag cacggattgc cgcagcggta tccagcgcgt ttgcaccggt cgccagcttg
     7561 atccccagac cggaagcagg ttggccgtta aactctgcga tgatgtcgta gttctcacca
     7621 cccagctcaa tcttcgccac atcacgcagc agtacgcggg aaccatcctg attcactttc
     7681 agcaggattt tgccgaactc ttcagtagag gtcagacgcg tctgagcaat aatagaggcg
     7741 ttaagctgtt ggcctttcac cggcggcgta ccaccgagct gacccgccgc aacctgggcg
     7801 ttctgcgctt tgatggcggt aatgacatca accggcgtta gctggaattt gttcagctca
     7861 ttcgggttca tccagatacg catcgcgtac tgtgaaccga acaactgaac atcacccacg
     7921 cccgacgtac ggctgatggc atctttcata ttcgccgcta cgtagtcaga gatatcctcc
     7981 tgcgtcatgg tgccatcggt gttgataacg ccgacaacca tcaggaagct gctggatgat
     8041 ttctcaacgc tcaccccttg ctgctgaact tcttgcggca gcaacggcat cgccagctgc
     8101 agtttgttct gcacctgaac ctgcgcgata tccgcatcag taccagactc aaaggtcagg
     8161 gtgatctgca cggtacccgt ggagtcactg ttagaggaca tgtacatcag gttatcgata
     8221 ccgttcatat tctgttcgat aacctgtgtc accgtgtcct gcactgtttt cgcatcagcg
     8281 ccggggtagg aggcggagat cgttactgcc ggcggtgcaa tcgtaggata ttgcgccacc
     8341 ggcagtttga ggatcgccag cccccctgcc aacatgatga taatggcgat cacccacgca
     8401 aaaatcgggc gatcgataaa gaaattaggc atgtcttaac ggctcctgtt taagttaaga
     8461 cttggactgt tcaggctgag caccgcttgc ggcttgctgg ttattatcag cggtaacttc
     8521 ttgtgctttt acctggacac caggacgcac tttctgcagc ccacttatta ctacgcgatc
     8581 gcctgctttc agaccttctg tcaccagcca cttatcgcca atagcctggc ttgcaacgat
     8641 cggacgggtt tccactttgt catccgcgcc aactaccagt acggtggcat caccacgcgg
     8701 cgtacgggtt acgccctgtt gcgggactaa aatagcgttt ggattaagcc cttcttccag
     8761 acgtgcgcgc acgaacatac ccggcagcag agtgtgatcc gggttcggga agatagcgcg
     8821 tagggtgata gacccagtgg tctgatcaac ggtaacgtca gagaattcca gcgtaccgtc
     8881 ctgcgggaac ttaatgccgt cactggtgat cagcgacact ttggctttgc cgttctcttg
     8941 tttcagcgtg ccattcgcca gttcctgttt caggcgaagg aagtcgttgc tggactgggt
     9001 cacatcaacg tagatcggat caagttgctg cacggttgcc agcacagtcg cctgaccgtt
     9061 ctgtaccaat gcgccttccg tcacgttcga cttaccaatg cgaccgctaa tcggagaggt
     9121 gactttggtg taagccagat tgatccgcgc agtttcaacg gcagctttcg ccgcagttac
     9181 cgcagcattc gcctgttgcg catcagccag agcctgatcg tacccttgct tactgatgta
     9241 ctgagtaccg agcagtttct gataacgatt caccgtcaat tgcgcgatat tggctgcagc
     9301 ctgggctttc gccagatcac ctttcgcact gtcgtatgtc gcctgatagg tcgcaggatc
     9361 aatctgatag agagagacac cagcttcgat gtcgctacct tctttgaaat tacgcttcag
     9421 gataatcccg ctaacttgag gacgaacttc tgcgatccgg taggcactgg tgcgacctgg
     9481 aagctcggtt gtgatctgca gaggttcagt tttgactgtt actacgccaa cggcgggcat
     9541 ctgctggcca ccttgttggg cctgtttgtc gtcacatcct gttagggcta agctgcctga
     9601 gagcatcaga acgaccgcca gaggcgtaaa ccctctgttt ttgttcatat gtaaacctcg
     9661 agtgtccgat ttcaaattgg tcaatggtca aaagttaata aacccattgc tgcgtttata
     9721 ttatcgtcgt gctatggtac atacattcac aaatgtatgt aaatctaacg cctgtaaatt
     9781 cacgaacata tggcacgaaa aaccaaacaa gaagcgcaag aaacgcgcca acacatcctc
     9841 gatgtggctc tacgtctttt ctcacagcag ggggtatcat ccacctcgct gggcgagatt
     9901 gcaaaagcag ctggcgttac gcgcggtgca atctactggc attttaaaga caagtcggat
     9961 ttgttcagtg agatctggga actgtcagaa tccaatattg gtgaactaga gcttgagtat
    10021 caggcaaaat tccctggcga tccactctca gtattaagag agatattaat tcatgttctt
    10081 gaatccacgg tgacagaaga acgacgtcga ttattgatgg agattatatt ccacaaatgc
    10141 gaatttgtcg gagaaatggc tgttgtgcaa caggcacaac gtaatctctg tctggaaagt
    10201 tatgaccgta tagaacaaac gttaaaacat tgtattgaag cgaaaatgtt gcctgcggat
    10261 ttaatgacgc gtcgggcagc aattattatg cgcggctata tttccggcct gatggaaaac
    10321 tggctctttg ccccgcaatc ttttgatctt aaaaaagaag cccgcgatta cgtcgccatc
    10381 ttactggaga tgtatctcct gtgccccacg cttcgtaatc ctgccactaa cgaataaccc
    10441 tgaatctgac tccaggattt ttcctggaca ttttcgtcgt tgctattctg gttcactgcg
    10501 tcgtgatatt cttgcggttt gactttttca ggtcgttctt caggttcaga aaccttcatt
    10561 catcatgact atgttccagt attacaaacg atcacggcat tttgtttttt cagcatttat
    10621 tgcttttgtt tttgtcttgt tatgccagaa cacggcgttt gcgcgggcgt catcgaatgg
    10681 tgatctgccg acaaaagcgg acctgcaggc gcaacttgac tcactaaata aacaaaaaga
    10741 tctttctgct caggacaaac tggtgcagca ggatctgaca gatacattag ccaccctcga
    10801 taaaatcgat cgcataaaag aagagacagt tcagctacgg caaaaagtcg ctgaagcgcc
    10861 ggaaaaaatg cgccaggcga ccgcggcgtt aacagcactt agcgatgtcg ataacgacga
    10921 agaaacgcgc aaaattctga gtacgctttc gttgcgccag ctggaaactc gcgttgccca
    10981 ggcgctggac gatttgcaaa acgcacaaaa cgatctggcg tcttataaca gccagctggt
    11041 ttcgttacag acgcagcccg aacgcgtgca aaatgcgatg tataacgctt cgcagcagct
    11101 gcaacaaatt cgcagtcgtc tggatgggac tgatgtcggc gagactgcct tacgtcccag
    11161 ccagaaagtg ttaatgcagg ctcagcaggc gttgctgaat gcggagattg accagcagcg
    11221 taaaagcctg gaagggaaca ccgtcttgca ggataccttg caaaagcaac gtgattacgt
    11281 gacggcgaac agcgcccgtc tggagcacca gttgcagctg ttgcaagaag cggtaaacag
    11341 caagcgcctg actttaactg aaaaaacggc gcaggaagcc gtctccccgg atgaagccgc
    11401 gcgtattcag gctaatccgc tggtgaaaca ggaactggaa attaaccagc agttaagtca
    11461 gcgtctgatt accgcgactg aaaacggtaa tcagttgatg cagcaaaaca ttaaagtcaa
    11521 aaactggctg gagcgggcgc tgcaatcgga acgcaatatt aaagagcaga ttgccgtcct
    11581 gaagggcagc ctgctgttgt ctcgtatcct ttaccagcaa caacaaacgc tgccctcggc
    11641 ggatgaactg gaaaacatga ccaaccgcat cgcggatttg cgtctcgaac agtttgaagt
    11701 taaccagcag cgtgatgcac tcttccagag cgatgcgttc gtcaacaaac tggaagaagg
    11761 tcacaccaac gaagtcaaca gcgaagttca cgatgcgtta ttgcaagtgg ttgatatgcg
    11821 tcgcgaattg ctggatcaac tcaacaaaca gttgggtaac cagctgatga tggccattaa
    11881 cctgcaaatc aaccagcagc agttaatgag cgtatcgaaa aacctgaaat ccatcctgac
    11941 tcagcaaatc ttttgggtga acagtaaccg tccaatggac tgggactgga tcaaagcgtt
    12001 cccgcaaagc ctgaaagatg aatttaagtc gatgaaaatc acggtgaact gggaaaaagc
    12061 ctggcctgcc gtttttatcg ctttcctcgc cggtttgccg ctgctgttga ttgccgggct
    12121 gatccactgg cgtctgggct ggctgaaagc gtatcaacag aaactggctt ccgctgtggg
    12181 ttccctgcgt aacgatagcc agctcaacac accaaaagcg atccttatcg acctgatccg
    12241 cgcgctgccg gtgtgcctga ttattctcgc ggttggcctg attctgttga ccatgcagct
    12301 caacatcagc gaactgctat ggtcgttcag taaaaaactg gcgattttct ggctggtgtt
    12361 tggcctgtgc tggaaggtac tggagaaaaa tggcgttgcc gtgcgtcact tcggcatgcc
    12421 ggagcagcag accagccact ggcgtcggca aattgtccgc atcagtctcg cgttgctgcc
    12481 tatccatttc tggtctgtgg tggcagaact ttccccgctg catctgatgg atgatgtgct
    12541 ggggcaggcg atgattttct tcaacctgct gctgattgcc ttcctggtat ggccaatgtg
    12601 ccgcgaaagc tggcgtgata aagagtcgca caccatgcga ctagtcacca ttaccgtgct
    12661 gtcgattatc ccgattgcat tgatggtgct gactgccacg ggttatttct acaccacgct
    12721 gcgcctctcc gggcgttgga ttgaaaccgt ttatctggtg atcatctgga acttgctcta
    12781 ccagacggta ttgcgcggct taagcgtagc ggcgcggcgt atcgcctggc gtcgtgcgct
    12841 ggcgcgtcgg cagaatctgg tgaaagaggg cgcagaaggt gccgaaccgc cggaagaacc
    12901 caccattgca ctggagcaag ttaaccagca gacgctgcgt attaccatgt tgctgatgtt
    12961 tgcgctgttc ggtgtcatgt tctgggcaat ttggtccgat ttgatcaccg tgttcagcta
    13021 tctcgacagc atcacgctct ggcattacaa cggcactgaa gctggcgctg cggtggtgaa
    13081 aaacgtcacc atgggcagtc tgttgtttgc gattatcgcc tcaatggtgg cctgggcgtt
    13141 gattcgcaac ctgcctggtt tactggaagt gctggtgctc tcgcgactga atatgcgcca
    13201 gggcgcgtcg tatgccatta ctaccatcct taactacatc attattgccg ttggtgcgat
    13261 gacggtgttc ggatcgctgg gcgtctcttg ggataaactc cagtggctgg ccgcagcatt
    13321 atccgtaggt cttggttttg gtttacaaga aattttcggt aacttcgtct ccggtttgat
    13381 cattctgttc gaacgtccgg tgcgtattgg cgatacggta accattggta gcttctcggg
    13441 gacggtaagt aagatccgta ttcgtgcgac aacgattacc gatttcgatc gcaaagaagt
    13501 gatcatcccg aacaaagcgt ttgttaccga gcgtctgatc aactggtcgt tgactgacac
    13561 tactacgcgt ctggtgatcc gtctcggcgt ggcctatggc tccgatctgg aaaaagtgcg
    13621 taaagtgtta ctgaaggcgg cgactgagca cccaagggtg atgcacgaac caatgccgga
    13681 agtcttcttt acggcatttg gtgccagcac gttggatcat gagctgcgtc tgtatgtgcg
    13741 tgaactgcgt gaccgtagcc gtactgtcga tgagctgaac cgtactatcg atcagctgtg
    13801 ccgtgaaaac gacatcaaca ttgcctttaa ccagcttgaa gtgcatctgc acaacgagaa
    13861 gggcgatgag gtgacggaag taaaacgcga ctacaaaggc gacgatccga caccagcggt
    13921 tgggtaataa ccaaaggggg cgacatttag ttgccccgag attactgaca aagtgcgcgt
    13981 tgttcatgcc ggatgcggcg tgaccgcctt atccggccta cgaaaccgca agaattcaat
    14041 atattgcagg agcggtgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgcc
    14101 cgcaatctta ggggacattt agcggcccca tttatttctc acttttccgc ctcatcatcg
    14161 tgcgttaatt tcttttcata atcacgcttt acaatatcca gcgcgcgcag cacggtactg
    14221 gcagggatct gattttcctc cagcagcaca atcaaatcga cagccagttt gacatcgtca
    14281 ggggcatttt caagtgacat actctttcca ttgctagcgg gttaagcgcg ctagcctgtt
    14341 ttcgattttt tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg cttccacttc
    14401 acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg ctctgcgttc
    14461 ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt tacgctgcca
    14521 gcgggaaatt ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg aggcttcacg
    14581 cgcgatggct tccagttgtg ccgccagatg ttccgccagc caggccactt gcggcagctg
    14641 ttgctgctca actgcatgac gaagcgcagc cagattatcg cccgcctcgt cgagacaaga
    14701 ctgtagtgtt gtcgcacgag tctgaaaaag atgcctgtcg aaacgagcac ttagcgtggc
    14761 gaactgtgac accggggcac aacgctgacg cagcgtagcg agctgtcctt ccagtttttc
    14821 cagcagcagg gcggttttca cgataagacc tcaatgaaaa tgataattgt tatgctaaag
    14881 tagccactat gtaagctgac aacactcaat atgcaacgaa tcattttaat catcattggc
    14941 tggctggcgg tagtgctggg tacgctgggc gtggtattac cggtattacc gacgacgccg
    15001 tttatcctgc tggcggcctg gtgctttgcc cgttcttccc cgcgctttca cgcctggttg
    15061 ctgtaccgct catggtttgg cagctatcta cgtttctggc agaaacatca tgcgatgccg
    15121 cgcggcgtca aaccgcgggc gattttgctt attttgctca cgtttgccat ttctctgtgg
    15181 ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg tttgcttttc
    15241 tatatgtggc gaattccggt gattgatgaa aagcaagaaa agcactgaag cacaacaatc
    15301 gcagttgcaa ttattgcgta cagccagtac attctggcgt tttcgagcac aggcgcaggc
    15361 ggtcaaaggt taaacaacag ttacttttga tacgtttaaa acgcgccgtg agtaccaccg
    15421 taacaagcag gcatacactt atgaccgcga ctgcacagca gcttgagtat ctcaaaaata
    15481 gcatcaaaag cattcaggac tacccaaaac ccggcattct tttccgcgat gtcaccagct
    15541 tactggaaga cccgaaagct tacgctctca gcatcgactt gctggttgag cgttacaaaa
    15601 atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt ggcgctccgg
    15661 tagctctggg tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa ctgccgcgtg
    15721 aaaccatcag tgaaaattac gacctggaat acggcaccga tcagctggaa atccacgttg
    15781 atgccatcaa accgggcgac aaagttctgg tggtggacga tctgctggca accggcggca
    15841 ctatcgaagc gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct gacgctgcgt
    15901 tcattatcaa cctgtttgat ctcggcggcg aacagcgtct cgaaaaacag ggcattacca
    15961 gctacagcct tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc tgcccacgct
    16021 acggacagca caagatgtgc attcagcctc gccgttctga cggggctgtg ttagcattac
    16081 cccttcgtga atccaccttc cagcgtttca gagcctgcca atgagttatc aggttttagc
    16141 ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc caggaacatg tgctgaccgc
    16201 actggcgaac ggcttgtcgt tagggcgtat tcatcatgct tatctttttt ccggcacccg
    16261 tggcgtcgga aaaacctcta tcgcccgact gctggcgaag gggctaaact gcgaaaccgg
    16321 cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc aggggcgctt
    16381 tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa gttgaagata cccgcgacct
    16441 gctggataac gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc tgatcgacga
    16501 agtgcatatg ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg aagagccgcc
    16561 ggagcacgtt aagtttctgc tggcgacgac cgatccacag aaattgccgg tgacgatttt
    16621 gtcacgctgt ctgcaatttc atctcaaggc gctggatgtc gagcaaattc gccatcagct
    16681 tgagcacatc ctcaatgaag aacatatcgc tcacgagccg cgggcgctgc aattgttggc
    16741 gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg accgaccagg cgattgccag
    16801 cggtgacggc caggtttcaa cccaggcggt cagtgcgatg ctgggtacgc ttgacgacga
    16861 tcaggcgctg tcgctggttg aagcgatggt cgagggcaac ggcgagcgcg taatggcact
    16921 gattaatgaa gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg aaatgctcgg
    16981 cttgttgcat cgtattgcga tggtacaact ttcgcctgct gcacttggca acgacatggc
    17041 cgccatcgag ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg atattcagct
    17101 ttactatcag acgctgttga ttggtcgcaa agaattaccg tatgcgccgg accgccgcat
    17161 gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat ccgcgtatgc cgctgcctga
    17221 gccagaagtg ccacgccagt cctttgcacc cgtcgcgcca acggcagtaa tgacgccaac
    17281 cctggtgccg ccgcaatcag cgccgcagca ggcaccgact gtaccgctcc cggaaaccac
    17341 cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg cagggagcaa ccaaagcaaa
    17401 aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg aataacgctg cgctggaaag
    17461 actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg ccatcggcgc tggaaaaagc
    17521 gccagccaaa aaagaagcgt atcgctggaa ggcgaccact ccggtgatgc agcaaaaaga
    17581 agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa catgaaaaaa cgccggaact
    17641 ggcggtgaag ctagcggcag aagccattga gcgcgacccg tgggcggcac aggtgagcca
    17701 actttcgcta ccaaaactgg tcgaacaggt ggcgttaaat gcctggaaag aggagagcga
    17761 caacgcagta tgtctgcatt tgcgctcctc tcagcggcat ttgaacaacc gcggtgcaca
    17821 gcaaaaactg gctgaagcgt tgagcatgtt aaaaggttca acggttgaac tgactatcgt
    17881 tgaagatgat aatcccgcgg tgcgtacgcc gctggagtgg cgtcaggcga tatacgaaga
    17941 aaaacttgcg caggcgcgcg agtccattat tgcggataat aatattcaga ccctgcgtcg
    18001 gtttttcgat gcggagctgg atgaagaaag tatccgcccc atttgatcgt aagcacagct
    18061 tacgttcgtc atccttaacg tgattgagag agaaacctat gtttggtaaa ggcggtctgg
    18121 gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat gcagaaaatg caggaagaga
    18181 tcgcgcagct ggaagttacc ggcgaatctg gcgcaggtct ggtaaaagtg accatcaacg
    18241 gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct gctggaagac gacaaagaga
    18301 tgctggaaga cctggtggct gcagcattca acgacgcagc acgtcgtatt gaagaaacgc
    18361 agaaagaaaa aatggcctct gtatcctccg gaatgcagct gccgcctggc tttaagatgc
    18421 cgttctgatg caaaccagcc cgctgttaac acagcttatg gaagcactgc gctgtctgcc
    18481 gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg ctgcttcagc gcgatcgtag
    18541 cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg tcggaaatcg gccactgcgc
    18601 cgattgccgc actttcaccg aacaggaagt ctgtaacatc tgttcgaatc cgcgtcgtca
    18661 ggaaaacggt caaatctgtg tggtggagag tccggcggac atctacgcca ttgagcagac
    18721 ggggcagttt tctggtcgtt attttgtgtt gatggggcat ctgtcaccgc tggacggcat
    18781 cggtccggat gatatcgggc ttgatcgtct ggaacagcgt ctggcagagg aaaaaatcac
    18841 tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa gctaccgcta actacattgc
    18901 cgagctttgc gcgcaatatg acgtggaagc caggcgaatc gctcatggcg taccggttgg
    18961 cggcgaactg gaaatggtcg atggcaccac gctgtcacac tccctcgccg ggcgtcataa
    19021 gattcgtttt taagcaaacg agagcaggag cacctgctct cgcttgaaat tattctccct
    19081 tgcccccatc tctccctcat cctgttttta accttaaaat ggcattattg aggtagacct
    19141 acatgaaagg acaagaaact cgtggttttc agtcagaagt gaaacagctt ctgcacctga
    19201 tgatccattc tctctattcc aataaagaaa tcttcctgcg tgagcttatc tctaacgcct
    19261 ccgatgcggc ggacaagctg cgtttccgtg cgctatctaa cccggacctg tacgaaggtg
    19321 atggcgaact gcgcgttcgt gtctctttcg ataaagacaa gcgcacgctg accatctccg
    19381 ataacggcgt ggggatgacc cgcgacgaag tgattgacca tctggggact atcgctaaat
    19441 ccggtaccaa atcattcctc gaatccctgg gttctgacca agcgaaagac agccagctga
    19501 tcggtcagtt tggtgttggt ttctactctg cgtttatcgt ggccgacaaa gtgaccgtgc
    19561 gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt ctgggaatcg gctggcgaag
    19621 gtgaatatac cgttgccgat atcaccaaag aagatcgtgg tactgaaatc accctgcatc
    19681 tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt gcgttccatc atcagcaaat
    19741 actccgacca tatcgcgctg ccggtagaga tcgaaaaacg cgaagagaaa gatggcgaaa
    19801 ccgttatctc ctgggcaaaa atcaacaaag cgcaggcgct gtggactcgt aacaagtcgg
    19861 aaatcaccga tgaagagtat aaagagttct acaaacacat cgcccacgac tttaacgatc
    19921 cgctgacctg gagccacaac cgcgttgaag gtaagcagga gtacaccagc ctgctgtaca
    19981 tcccgtccca ggctccgtgg gatatgtgga accgtgatca taaacacggc ctgaaactgt
    20041 acgttcaacg cgtgtttatc atggacgacg ctgaacagtt catgccgaac tatctgcgct
    20101 tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa cgtttcccgt gaaatcctcc
    20161 aggacagcac ggtaacgcgt aacctgcgca atgcgctgac caagcgtgtg ctgcaaatgc
    20221 tggaaaaact ggcgaaagac gacgcggaaa aataccagac cttctggcaa cagtttggcc
    20281 tggtactgaa agaaggtccg gcggaagatt tcgctaacca ggaagcgatc gccaaactgc
    20341 tgcgttttgc ttctacttat accgattctt ctgcgcagac cgtgtctctg gaagactacg
    20401 tttcccgcat gaaagaaggg caggagaaaa tctactacat cactgcagac agctatgcgg
    20461 cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa aggcatcgaa gttctgctgc
    20521 tttccgaccg catcgatgag tggatgatga actatctgac tgagttcgac ggtaaaccgt
    20581 tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact ggctgacgaa gttgatgaga
    20641 gcgcgaaaga agcggagaaa gcactgactc cgttcatcga ccgcgtgaaa gctctgctcg
    20701 gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac cgatacgcca gcgatcgttt
    20761 cgaccgacgc ggacgaaatg agcactcaga tggcgaaact gttcgctgcg gcgggccaga
    20821 aagtgccaga agtgaaatac atcttcgaac tgaacccgga tcacgtactg gtgaaacgtg
    20881 cggcagatac tgaagatgaa gccaagttca gcgagtgggt agaactgctg ctggatcagg
    20941 cgctgctggc agaacgcggc acgctggaag atccgaacct gtttattcgt cgtatgaatc
    21001 agctgctggt ttcctgatgt catgccggat gacctttgtg tcatccggca tttttctttt
    21061 catcatctgt actttccgca aattatctcg ccattaaccg tttcagcctc aggtgccttt
    21121 cttgaggcaa tcgcctgttg gtggtatcgt ttatcgcttt ttcaaaaaaa tcgacacatt
    21181 ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc tccgggcgcg gggaaaggga
    21241 ctcaggctca gttcatcatg gagaaatatg gtattccgca aatctccact ggcgatatgc
    21301 tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca agcaaaagac attatggatg
    21361 ctggcaaact ggtcaccgac gaactggtga tcgcgctggt taaagagcgc attgctcagg
    21421 aagactgccg taatggtttc ctgttggacg gcttcccgcg taccattccg caggcagacg
    21481 cgatgaaaga agcgggcatc aatgttgatt acgttctgga attcgacgta ccggacgaac
    21541 tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc gtctggtcgt gtttatcacg
    21601 ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt taccggtgaa gaactgacta
    21661 cccgtaaaga cgatcaggaa gaaaccgtgc gtaaacgtct ggttgaatac catcagatga
    21721 ctgcaccgct gatcggctac tactccaaag aagcggaagc gggtaacacc aaatacgcga
    21781 aagttgacgg taccaagcag gttgctgaag ttcgcgctga tctggaaaaa atcctcggct
    21841 aatcaagccg cagcagatct gctttcgggc aggtctgctt atccacctca ggcaatacct
    21901 ctcacagcaa ttagttcttc ttcctcactt ttccgctaca attatcaaca agttgaatcg
    21961 ataagaggcg gtaatgcgtc agactaaaac cggtatcctg ctggcaaacc tgggtacgcc
    22021 cgatgccccc acacctgaag cggtaaaacg ctatctgaaa caatttttaa gcgacagacg
    22081 cgtggttgat acctcacggt tgttatggtg gccgttgctg cgcggcgtga ttttgccgct
    22141 gcgctcgccg cgtgtggcga agctgtacgc ctctgtctgg atggaagacg gctcgccgct
    22201 gatggtttac agccgccagc aacagcaggc gttggcacaa cgtttaccgg atacgccagt
    22261 ggcgctgggc atgagctacg gctcgccatc actggaaagc tccgtcgatg aactcctggc
    22321 agagcatgta gatcatattg tggtgctgcc gctttatccg caattctcct gttctacggt
    22381 cggtgcggta tgggatgaac tggcacgcat tctggcgcgc aaacgtagca ttccggggat
    22441 atcgtttatt cgtgattacg ccgataacca cgattacatt aatgcactgg cgaacagcgt
    22501 acgcgcttct tttgccaaac atggcgaacc ggatctgctg ctgctctctt atcatggcat
    22561 tccccagcgt tatgcagatg aaggcgatga ttacccgcaa cgttgccgca caacgactcg
    22621 cgaactggct tccgcactgg ggatggtacc ggaaaaagtg atgatgacct ttcagtcgcg
    22681 ctttggccgg gaaccctggc tgatgcctta taccgacgaa acgctgaaaa tgctcggaga
    22741 aaaaggcgta ggtcatatac aggtgatgtg cccgggcttt gctgcggatt gtctggagac
    22801 gctggaagag attgccgagc aaaaccgtga ggtcttcctc ggtgcgggcg ggaaaaaata
    22861 tgaatatatt ccagcgctta atgccacgcc ggaacatatc gaaatgatgg ctaatcttgt
    22921 tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg ccgtcgcgaa gagcctcgtc
    22981 ggcggttttc atcatccgtg aataatgcaa aaaggcatgc agcgtacctg ggtagagttt
    23041 gaactcacag ggctgctgat gcgccgctaa cgtctagtaa agcagacggc tgtcatccag
    23101 cagcggatcg aactccgccc cggcaataaa acagggcgga acttcgcgag tgagatcatt
    23161 attaaacaga cagtaatacg gtgactcgcg gtccgcgtcg ttgcttaaat acgcctcttc
    23221 atacatctgc aaatcctgtt gcgttaagcc atcccagaca ccgcccaaca gacgacgagt
    23281 cacggaatcc cgtaatccgt aaagcccata ccacagcaaa acacccgcaa ttttaccgca
    23341 atcgatctgt ttatcacgca accacaacgc actggcgagc gccagcatgg cacctgcgga
    23401 atcaccggca aagccaatgc gggacatatt gatttgataa tcctccgcct gctggtggaa
    23461 ataacaacaa gcagccacaa tttcctctat cgcttgtgga aaacgcgctt caggtgaaag
    23521 ggtgtaatta ataccaatca ccgtacattg gctgtagctt gccagcaggc gcatgatgcg
    23581 atcgtgggta tcgagattgc cgagaataaa accgcctcca tgcaaataaa atagcgtcgc
    23641 cgggctatct ggctgcggac aaaagagacg tgtttccacc tgcccatatt ttgttggaac
    23701 catgtaagct ctggttgcca tttctggagc gcccgcattc cagaatcggc gctcaagcgt
    23761 gtaatactgt cgttgctcag caatcgttcc cgttgcgggc caggacggta aatccggctg
    23821 aagagtattc acaacggtct tcatttcagc agaaataagg tccagaacag gtagtttgtt
    23881 ttccggcttc atacaaaaac tcctttcaaa ttacgtcatt gtaaggaacc actgccatga
    23941 aaatgcgatc ccgcctgctg atattgaaac tggctgcgtc tcgcgcgctc ccgtcagatt
    24001 gtgttaacat tcgccgctca gttaaccacc cgtaaaaaca accatgaaat ttcccggtaa
    24061 acgtaaatcc aaacattact tccctgtaaa tgcacgcgat ccgctgcttc agcaattcca
    24121 gccagaaaac gaaaccagcg ccgcctgggt cgtaggtatt gaccagacgc tggtcgatat
    24181 tgaagcgaaa gtggatgatg aattcattga gcgttatgga ttaagcgccg ggcattcact
    24241 ggtgattgag gatgacgtag ccgaagtgct ttatcaggag ctaaaacaga aaaacctgat
    24301 tacccatcag tttgcgggtg gcaccattgg taacaccatg cacaactact cggtgctcgc
    24361 ggacgaccgt tcggtgctgc tgggcgtcat gtgcagcaat attgaaattg gcagttatgc
    24421 ctatcgttac ctgtgtaaca cttccagccg taccgatctt aactatctac aaggcgtgga
    24481 tggtccgatt ggtcgttgct ttacgctgat tggcgagtcc ggggaacgta cctttgctat
    24541 cagtccaggc cacatgaacc agctgcgggc tgaaagcatt ccggaagatg tgattgccgg
    24601 agcctcggca ctggttctca cctcatatct ggtgcgttgc aagccgggtg aacccatgcc
    24661 ggaagcaacc atgaaagcca ttgagtacgc gaagaaatat aacgtaccgg tggtgctgac
    24721 gctgggcacc aagtttgtca ttgccgagaa tccgcagtgg tggcagcaat tcctcaaaga
    24781 ccacgtttct atccttgcga tgaacgaaga tgaagccgaa gcgttgaccg gagaaagcga
    24841 tccgttgttg gcatctgaca aggcgctgga ctgggtagag ctggtgctgt gcaccgccgg
    24901 gccaatcggc ttgtatatgg cgggctttac cgaagacgaa gcgaaacgta aaacccagca
    24961 tccgctgctg ccgggcgcta tagcggaatt caaccagtat gagtttagcc gcgccatgcg
    25021 ccacaaggat tgccagaatc cgctgcgtgt ctattcgcac attgcgccgt acatgggcgg
    25081 gccggaaaaa atcatgaaca ctaatggagc gggggatggc gcattggcag cgttgctgca
    25141 tgacattacc gccaacagct accatcgtag caacgtacca aactccagca aacataaatt
    25201 cacctggtta acttattcat cgttagcgca ggtgtgtaaa tatgctaacc gtgtgagcta
    25261 tcaggtactg aaccagcatt cacctcgttt aacgcgcggc ttgccggagc gtgaagacag
    25321 ccaggaagag tcttactggg atcgttaagt tatcgtcggt tcgtaggcca gataaggcgt
    25381 tcacgccgca tctggcattt ggctctcgat gcctgatgcg acgctggcgc gtcttatcat
    25441 gcctacatat ttttcatatt ttacatccgg caaccaccgt ttaccccgtc accacttcac
    25501 ccgccggtgg cgtttccagc agttccagca tagtacgggc aatttcacgt tcacccatca
    25561 ctacctgatt cgcgccacgt tcggtgatat acgtcacttc atcgtcataa tgggcgcggg
    25621 cgataatctc aatatccggg tttttcgcgc gggcagacgc cacaatctca cccgcttcat
    25681 aaccgttggg gatcgtcagg atcagccatt ttgcacattc cagatgtgcc agttgcataa
    25741 tttcttcgtt cgctgcattg cccaatacag cgcggacccc acgctctcgc agctcatcaa
    25801 cacgggttcg tgacgtctca atcaccacca gcggaatatc agaggcgagc aatttctccc
    25861 ccagcaggct gcctacacga ccgtaaccca ccaatagcgc atggttgcaa atatccactg
    25921 ggatctgttt ctcttcttcg attgcctctt ccagcgtctg ctcttccagc gtttcggtct
    25981 tcgccagata tttctccagt agtgcgaaca gtaccgggtt gagcataatc gacaggatcg
    26041 cccctgccag taccaggttt tgtccggcct gcggcagtaa attcaatgcc attcccagtc
    26101 ccgccaggat aaacgcgaac tcaccaatct gcgccaggct ggcggcgatg gttaatgccg
    26161 tacgttggga gtgaccaaac agacggacaa ggaacagggc cgcgaccgat ttaccaaaca
    26221 gaataattgc cagcgtcgcc agcactgcca gcggttgctg aatcagaatt aacggatcaa
    26281 acaacatccc gactgagaca aaaaacagca ccgcaaacgc gtcgcgcaat ggcaacgtat
    26341 cgtgggcggc acggtgactc aattcagact cgttcagtac catcccggca aagaacgcac
    26401 cgagtgcaaa ggagacatca aacagctcta ccgcaccaaa ggcaatccct aacgccagcg
    26461 ccagcactga cagggtaaac agctcgcgag aaccggttgc cgcgctgcgt gccataatcc
    26521 acggcaccag acggcgacct accagcatca taatggcgat aaatgcgatc accttgccga
    26581 tggtgatccc catatcgact gcaagagtgg caaagcccac atcgccctgt tccatcattc
    26641 ctgccactgc gggcagcaac accagcgtca gaaccatcac caggtcttcc acaatcaacc
    26701 aaccgatggc gatttgccca cgctgactgt ctattaattg ccgttcttca agtgcgcgca
    26761 gtaacaccac ggtactggcg gtggaaagac ataagccgaa cacgataccg gtcattaacg
    26821 accagcccag cacggcggag agcgccatac ccagcagcgt cgccacggct atctgggcga
    26881 tcgcgccggg aatggcgatg gactttaccg ccatcaaatc cttgagcgaa aaatgcaaac
    26941 cgacgccgaa catcagcaaa ataacgccca gttcagccag ttccggcgca agcttggtat
    27001 cggcaacaaa gcccggagtg aatggtcctg ccagcacacc cgctaacaga tatcccacca
    27061 gaggagaaat acgtagttta ttggccagca tgccgaggat aaaggcgagc acaaggccgc
    27121 caacaatggt ggtgataagc ggggtggcgt gatgcattcc gtctcctttt cctggtggtt
    27181 attgtccatt tttggctggg aaaaccaaaa ttacaggtaa tagtttatga caatttcatt
    27241 gatgatgttt atgaataatt gttgaatttt gcagaaaaat ggaattagct gcaaaaaaag
    27301 cacggttcgg aaaacagaag gcggtttaac agaggaaagg cttatggcga ctgtaaaacg
    27361 atgcagccaa agtttgcctt tggctgcaat gaaatcagtc tttatgccgg ttatcaggca
    27421 ggaatatggt caacatcccc aatagtggca ggaaagcaca gattttatag actaactcga
    27481 tgctggtgtg atcggcgata agccccagaa ctgccgctcc cagacctccc atgccgaaag
    27541 caaaaccgaa aaagagtcca gaaaccatac cgatacgtcc cggaagtagc tcctgagcgt
    27601 agaccagaat ggcagagaat gccgaagcga ggataaatcc aataatcacc gttaaaaccc
    27661 ccgtccagtg caggcgggcg tagggtaaaa tcagcgtaaa cggcgcaacg ccgaggatag
    27721 agccccaaat cacatatttc cgtccaattt tatcccctac aggcccgccg atcaccgtac
    27781 ctgccgcaac ggcaaacagg aaggcaaaca gatgaagctg ggcattctgg atagataatc
    27841 cgaatttttg catcagataa aaggtgtaat agctgctgat gctcgccata tagaaatatt
    27901 tcgagaaaat gaggattaac agaatgctga ctgccagtac cactttgttg cgcggcagtg
    27961 gattgattat cgtcgctttg ggttttcctt tattcattcg gtgctgtgcc gagtaccaac
    28021 ggctaatttg cgccaacacc acgatcgcca gcagtgccgc aagcacaaac caggcaacgt
    28081 tgcctttgcc ataaggctcg ataatcaccg ccgccagcaa gggtcccagg gaactgccaa
    28141 agttgccgcc gacctgaaag atagattgcg ccaggccatg ccgcccgccg gaagccatac
    28201 gggctacgcg agaagattcc ggatgaaaga ccgatgaacc ggtaccgacc agcgccgctg
    28261 ccagcagaac tgcgccaaaa ctgcccgcca gcgcaagcag caccagacca cttaaggtaa
    28321 agcacatgcc aattggcaac gaccacggca tcggatattt atcggtccag tagccgacca
    28381 ctggttgcag tagcgaagag gcgagctgga aggtgagggt tatcatgcca atctgcataa
    28441 atgtcagaga aaattctgac tgaagcagcg gataaatcgc cagaatcagc gattggatca
    28501 tgtcgttcag cagatgtgag aggctgatag cacctaaaat accaaacgat gttcgggcct
    28561 tggtcgttga cgcagccgcg cccgccacag gctgggtttg ttcactcatt gccataggaa
    28621 agtcactttt tcagggttgc gatgtaaaga atgatcttat ttatgattat tacccgacta
    28681 acatacctgt atgcgtcgtc tgaaggaagt ctcaacgccg aatacagaat ttctaatctg
    28741 gatgcagatt tatcttcacc ggacgcagac ttgtctatga tgttgcgtca tactattttt
    28801 caacacgttg aaatcaggtc agggagagaa gtatgaaatt attgcagcgg ggcgtggcgt
    28861 tagcgctgtt aaccacattt acactggcga gtgaaactgc tctggcgtat gagcaggata
    28921 aaacctacaa aattacagtt ctgcatacca atgatcatca tgggcatttt tggcgcaatg
    28981 aatatggcga atatggtctg gcggcgcaaa aaacgctggt ggatggtatc cgcaaagagg
    29041 ttgcggctga aggcggtagc gtgctgctac tttccggtgg cgacattaac actggcgtgc
    29101 ccgagtctga cttacaggat gccgaacctg attttcgcgg tatgaatctg gtgggctatg
    29161 acgcgatggc gatcggtaat catgaatttg ataatccgct caccgtatta cgccagcagg
    29221 aaaagtgggc caagttcccg ttgctttccg cgaatatcta ccagaaaagt actggcgagc
    29281 gcctgtttaa accgtgggcg ctgtttaagc gtcaggatct gaaaattgcc gttattgggc
    29341 tgacaaccga tgacacagca aaaattggta acctggaata cttcactgat atcgaatttc
    29401 gtaagcccgc cgatgaagcg aagctggtga ttcaggagct gcaacagaca gaaaagccag
    29461 acattattat cgcggcgacc catatggggc attacgataa tggtgagcac ggctctaacg
    29521 caccgggcga tgtggagatg gcacgcgcgc tgcctgccgg atcgctggcg atgatcgtcg
    29581 gtggtcactc gcaagatccg gtctgcatgg cggcagaaaa caaaaaacag gtcgattacg
    29641 tgccgggtac gccatgcaaa ccagatcaac aaaacggcat ctggattgtg caggcgcatg
    29701 agtggggcaa atacgtggga cgggctgatt ttgagtttcg taatggcgaa atgaaaatgg
    29761 ttaactacca gctgattccg gtgaacctga agaagaaagt gacctgggaa gacgggaaaa
    29821 gcgagcgcgt gctttacact cctgaaatcg ctgaaaacca gcaaatgatc tcgctgttat
    29881 caccgttcca gaacaaaggc aaagcgcagc tggaagtgaa aataggcaaa accaatggtc
    29941 gtctggaagg cgatcgtgac aaagtgcgtt ttgtacagac caatatgggg cggttgattc
    30001 tggcagccca aatggatcgc actggtgccg actttgcggt gatgagcgga ggcggaattc
    30061 gtgattctat cgaagcaggc gatatcagct ataaaaacgt gctgaaagtg cagccattcg
    30121 gcaatgtggt ggtgtatgcc gacatgaccg gtaaagaggt gattgattac ctgaccgccg
    30181 tcgcgcagat gaagccagat tcaggtgcct acccgcaatt tgccaacgtt agctttgtgg
    30241 cgaaagacgg caaactgaac gaccttaaaa tcaaaggcga accggtcgat ccggcgaaaa
    30301 cttaccgtat ggcgacatta aacttcaatg ccaccggcgg tgatggatat ccgcgccttg
    30361 ataacaaacc gggctatgtg aataccggct ttattgatgc cgaagtgctg aaagcgtata
    30421 tccagaaaag ctcgccgctg gatgtgagtg tttatgaacc gaaaggtgag gtgagctggc
    30481 agtaatccga aagtgccgga tgtttgcatc cggcacaatg cttaatcgcg gcgggcgata
    30541 tcagcaaatt tggcatcgag gatctttgcc agatcgcctg ccgccagttc gatatccagt
    30601 ccgcgcttgc cgccggaaac ataaatagtg gcaaattctt gtgcgggggc gtcgataatc
    30661 gttggcagac gttttttctg ccccagtggg ctaatccccc caaccaggta tcccgtcgaa
    30721 cgctgcgcga ccatcggatc ggccatctca actttcttgg cacccagcgc ttttgctact
    30781 tttttaagat ccagttgacc tgcgaccggc gtaacggcca cggcaaggtg tttcatatca
    30841 ccgttcactg ccaccagcag cgttttgtag acctgatccg gattcaaacc taattttttg
    30901 acgacttcat cgccaaaatt ggtttcagcc ggatcgtgct cgtaggtatg gatttgaaac
    30961 gaaatcttgt ttttttcgag taatttaact gcgggtgtca tggtaatttt cctgccttaa
    31021 acaaaaaaac gaagcaagca tacgcctgat ggcggtccaa aaaaaagagt catcttgcct
    31081 aagagtattg gcaggatggt gagattgagc gacaatcgag ttacaccgtc gcttaaagtg
    31141 acggcataat aataaaaaaa tgaaattcct ctttgacggg ccaatagcga tattggccat
    31201 ttttttagcg caacatttgc ggcaaattcc cttctccata caggtgtagt gcaccgaccg
    31261 cgaccacata tcgccccggc ggcatggcgc gtaatttatc ccgccaggcg agatttcgct
    31321 gatgcatcag cacatcgtac agcgactgac tgaacgtatt gggcagcgtt atatcattat
    31381 tttgcggcgg tgcattcagc caccagctca tcatttgttg cagcaaccgt gcgttggtat
    31441 gccagtgggt cagcgtatcg tccagcagcg ccagtccttt gtcagggagc tggagcaaca
    31501 tggcaatctg gttttcagcc ccttccagtt caatcacggg tttatgttgt tgcttcgccg
    31561 cctgcaatag ctggtaatcg ataccgtatt ctgcccgcag ccccagtttt tgtgcctgcg
    31621 tcgcctgaag aaccatcgcg atttgccaca gcggttgggt agaaaagagt gagggagaaa
    31681 tgcccatctc ctggctaatg tgctgcaggt tttggagttg ctcctcgcta atgcgctctt
    31741 ccagcgcctc gcaggcaggc aaattagcaa aaggcgtatc gctggtggaa acatccgcct
    31801 cgacgatcag cgcatcggcg tttttgagct ttttgagcaa acgggtgggc aggggagcca
    31861 tatcgtggct acccatatga atactgccaa tcagatgaaa atggcgattg ccggggaggg
    31921 tgatatcgat ggcaggccag gtgtaatgat taccgcgcag cgcggcccaa agcgttttta
    31981 cccggtacaa cagatccata cgacctccct ttgtgaaata tcatgctagc gcgcggtgag
    32041 ggatggcgca aatggagttg gggacttgtt taatgtgttt gtatgattca gtatgttctt
    32101 gcatcgctat tcacagggaa gcaacagtta aaaaccatga aacaggcaac aagaaaaccg
    32161 acgacacccg gagatattct tctctatgaa tatctggaac cgctcgattt gaaaatcaat
    32221 gagttagcag agttgctgca tgttcatcgt aatcgcgtca gtgcactgat caataacaat
    32281 cgtaaactca ccactgagat ggcattccgt ctggcgaaag tttttgatac cacagtcgat
    32341 tttaggctaa acctccaggc ggcagttgat ctttgggaag ttgaaaacaa catgcgcacc
    32401 caggaagaat tgggacggat tgaaacagtg gctgaatatt tggcacgccg tgaagagcgt
    32461 gcaaaaaagg tcgcgtaagg ccaaaacatc gcttcatctc gtaggccgga taaggcgttc
    32521 acgccgcatc cggcatccga gcaccattgc ctgatgcgac gcttacgcgt cttatcaggc
    32581 ctacaaacct gtgcctgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcagtca
    32641 tgcgtcgatg ccaaatgcgc cgccctaaag cagcgcatcc gcaatgacgt acttattcct
    32701 tcggtttaaa ccgcagcaac cggttggcgt tacttactac ggtaattgac gagagcgcca
    32761 ttgccgctcc ggcaactacc gggttaagca gtgttccagt aaacggccac aaaataccgg
    32821 cggcgaccgg aataccgatg ctgttgtaga taaaggcacc gagcaggttc tgcttcatgt
    32881 tgcgcagcgt tgcgcgggaa atcgcgagcg catccgcaac gcccatcagg ctatggcgca
    32941 tcagggtaat cgccgcggtt tcaatggcaa catcactgcc gccacccatc gcaatgccga
    33001 catccgcctg ggccagcgct ggcgcgtcgt taatgccgtc gcccaccatt gccacctgac
    33061 gtccttcact ttgcagacgt ttgatcgctt cggctttacc gtccggcagc actccggcaa
    33121 tcacttcatc aattccagct tctttggcga tcgcattggc ggtggttggg ttatccccgg
    33181 tcaacatcac cagacgatat cccgctttat gcaggcgttg cagcgccgcc acgctatcac
    33241 tacgcaacgg atcgcgtaca gccagcaggg ctactgcttt cccgtcaatc gccagcagca
    33301 caggcgttgc tccttgcgat gcctgagctg aaatatcggc ttcaatcgct ttagtatcga
    33361 cttgctgatc atttaacaac gcctgattac ccagcaataa cgcatgacct tctgcttcac
    33421 cgctcacgcc cagcccgcgc aatgtgcgga aaccgttgac ctgcggtagc tgcatatcgc
    33481 ctgctttatc aaggatcgct cgtgccaatg gatggctgga accttgctcc agcgccgccg
    33541 ccagacgcag cgcctgcgct tcgtcaacat cagcaaatgt tttcactgcg acaacctgcg
    33601 gcttcccttc ggtcagcgtc ccggttttat cgaacacgac ggtatcgagt gtactggcgc
    33661 gttgcagcgc gtcggcgtcc cgcaccagca cgccaaactc agccgcccgc ccgacgccgg
    33721 aaataatcga catcggcgtc gccagtccca gcgcacacgg gcaggcgata atcagcaccg
    33781 tagtggcaat caccagggta tagacaatct gcggtgccgg accaaagaaa taccagattg
    33841 ccgcactgac aagcgcaata accactacta ccggcacaaa tatggctgag attttatccg
    33901 ccagctgacc gatttctggc ttgctgctct gggcctggcg caccatgcga atgatccttg
    33961 atagcgtggt atggctgcca accgcactgg cacgaaacag cacactgccg tcctgtacca
    34021 ctgtcccggc atggacgcta tcgccttcgc ctttttgctg cgggattggt tcgcccgtca
    34081 gcatcgcttc atccagccat gcttcgccct gggtaatctc gccatctacc ggcacgcgat
    34141 cgccggtcgt caggcgcagc aacatacctg gctgcacttc tgccagaggc acgctttttt
    34201 caccttcgtc agtaaccagg cgcgccgtcg gtggggttaa atcgagtaat ttctccagtg
    34261 ccttagaaga acgctgacgt gcgcgtgctt ccagcatatg gccgagattg atcagaccga
    34321 taatcatcgc actggcttca tagtaaagat gtcgcgcttc catcgggaac cactgcggcc
    34381 acaggttgac gctcatcgaa tagagccacg ccacgcccgt tcccagcgcc accagcgtat
    34441 ccatcgtcgc tgcaccgttc agcaggcttt tccatgcact gcggtaaaaa tggccgctgg
    34501 cgaaaaccat caccgccagg gttatcaggc cgataaccaa ccacaggctg cggttgtcgg
    34561 cggtgactat catgttatcg ccgatcatcc cccagaccat caccgggata cccaccgcca
    34621 gtgcgacaat tgcctgccag cggaagcgct tcatcgtagc gacggcggtt tcttgctggc
    34681 gttcgcggcg tttcgcgtca tcttcaatcg cttccgcgcc gtagcccgct ttttccaccg
    34741 cctgcactaa atcttgtggg gaggcactgc ccatcaccag cgcagtacgc tccgccaggt
    34801 ttacccgtgc ctgagtgacg cccggtacgc tttgcagcgc attttgtacg cgggtgacac
    34861 agctggcgca gctcatgccg ctcagcagca actgctggct gtcatcgtca tcggcggtcg
    34921 ctgccggaag cgcctcagaa accgctgtca gtgcttccga cgggattgat gactccgcca
    34981 gcggtttagc ctttgggtgg cttacagatg cgtcataacc cgcttgtttg atggtttcga
    35041 tcagctgttc tgcactggca gtcccggtaa cgtgcgcttc agtgatagac acatccgctt
    35101 gttccacatc cgggcgctgt tcaagacttt ctttcacgcg tttaacgcag tgaccgcagg
    35161 acaggccgtc caggttcagg tcgatagttt gtgacataaa acactccttt aagacagttt
    35221 tgactggctg tgataaaggt taaaccttcc agcaagggga aggtcaagaa attaataaac
    35281 caggcgggta aaagtccgta aagattaaaa aatcggctcg atttgcatca ggattagaca
    35341 tttatttctt tgttttcctg tagttaagtt gcgggtgcta agttaaatca ggatgcctga
    35401 aaatcggcac tggggtgagg aattacctcc cgcatctata aaaaggagtt aacaaaagat
    35461 gttagatgca aacaaattac agcaggcagt ggatcaggct tacacccaat ttcactcact
    35521 taacggcgga caaaatgccg attacattcc ctttctggcg aatgtaccag gtcaactggc
    35581 ggcagtggct atcgtgacct gcgatggcaa cgtctatagt gcgggtgaca gtgattaccg
    35641 ctttgcactg gaatccatct ctaaagtctg tacgttagcc cttgcgttag aagatgtcgg
    35701 cccgcaggcg gtacaggaca aaattggcgc tgacccgacc ggattgccct ttaactcagt
    35761 tatcgcctta gagttgcatg gcggcaaacc gctttcgcca ctggtaaatg ctggcgctat
    35821 tgccaccacc agcctgatta acgctgaaaa cgccgaacaa cgctggcagc gaattttaca
    35881 tatccaacag caactggctg gtgagcaggt agcgctctct gacgaagtca accagtcgga
    35941 acaaacaacc aacttccata atcgggccat tgcctggctg ctgtactccg ccggatatct
    36001 ctattgtgat gcaatggaag cctgtgacgt gtacacccgt cagtgctcta cgctcatcaa
    36061 tactgttgaa ctggcaacgc ttggcgcgac gctggcggcg ggtggtgtga atccgttgac
    36121 gcataaacgc gttcttcagg ccgacaacgt gccgtacatt ctggccgaaa tgatgatgga
    36181 agggctgtat ggtcgctccg gtgactgggc gtatcgcgtt ggtttaccgg gcaaaagcgg
    36241 tgtaggtggc ggtattctgg cggtcgtccc tggcgtgatg ggaattgccg cgttctcacc
    36301 accgctggac gaagaaggca atagtgttcg cggtcaaaaa atggtggcat cggtcgctaa
    36361 gcaactcggc tataacgtgt ttaagggctg atcatgatga acacggaagg taataacggt
    36421 aacaaacccc tcggtctatg gaacgtcgtt tccatcggta ttggggcaat ggtgggggcg
    36481 gggatcttcg cgctgctggg gcaggctgcg ttgctaatgg aagcctcgac ctggggcgcc
    36541 tttgcttttg gcggtattgt ggctatgttt tccggttatg cctatgcgcg tctgggggcg
    36601 agctatccca gcaatggcgg tattatcgac ttttttcgtc gcggattagg caacggcgtc
    36661 ttttcgctag cgctctcgtt gctatacctg ttgacgctgg cggtgagcat cgccatggtt
    36721 gcccgtgctt ttggcgctta tgccgtgcag tttttgcatg aaggcagcca ggaggagcac
    36781 cttattttgc tctacgcgtt ggggatcatt gcggtgatga cgcttttcaa ctccttaagc
    36841 aaccatgcgg tagggcggct ggaagtgatc ctcgtcggca ttaaaatgat gatcctgtta
    36901 ttgctgatta ttgccggtgt ctggtcgctg caaccggcgc atatttccgt ctctgcgccc
    36961 cccagctccg gtgcgttctt ctcctgtatt gggataacct tccttgccta tgcgggcttt
    37021 ggcatgatgg cgaacgcggc ggataaagtg aaagatccgc aggtcattat gccacgggcg
    37081 tttctggtgg cgattggcgt taccacgttg ctttatatct cgctggcgct ggttttgctt
    37141 agcgatgtat cggcattaga gttagaaaaa tatgctgata ccgccgtagc gcaggctgct
    37201 tctccgctgc tcgggcatgt gggttatgtg atcgtcgtca tcggcgcttt actggcgacg
    37261 gcttcaggca ttaacgcgaa cctgttcgcc gtgtttaaca tcatggacaa catgggcagc
    37321 gaacgcgaac tgccgaagct aatgaataaa tccctgtggc ggcagagtac ctggggcaat
    37381 atcatcgtcg tggtgttgat aatgctgatg acggcggcgc taaatttaga ctcactcgcc
    37441 agcgttgcca gcgccacctt tttgatctgc tatctggcgg tgtttgtggt ggcgatccgc
    37501 ctgcgtcatg atattcacgc ctcgttgccg attcttatcg ttggtacgtt ggtgatgttg
    37561 ttggtgatcg ttggctttat ctacagcctg tggtcccagg gcagccgtgc gttgatatgg
    37621 attattggtg caatcttact cagccttatt gtggcaatgg tcatgaagcg taataaaacc
    37681 gtataacacc tctctgtgcg cagtacttcc tgtattattg tggtggcggt cgatattcgc
    37741 actggcaaaa aaacgtgctt gaatatctgt tgaaaccctt taacaaagca caggaggcgt
    37801 tgcgcgaacg atgaacatca gcgatgtagc aaaaattacc ggcctgacca gcaaagccat
    37861 tcgcttctat gaagagaagg ggctggtgac gccgccgatg cgcagcgaaa acggttatcg
    37921 cacctacacg cagcagcatc tcaacgaact gaccttactg cgccaggcac ggcaggtggg
    37981 ctttaacctg gaagagagcg gcgagctggt gaatctgttt aacgacccgc agcggcacag
    38041 cgccgacgtc aaacggcgca cgctggagaa ggtggcggag atcgaacgac acattgagga
    38101 gctgcaatcc atgcgcgacc agctgctggc actggcgaat gcctgccctg gcgatgacag
    38161 cgccgactgc ccgattatcg aaaatctctc cggctgctgt catcatcggg cagggtgatt
    38221 aagacgagac ggctcggata tgtagggtta tcccttctat cgcaatgact tcaacatgcg
    38281 tacctgcacc gagatcctcg ctggcgctga caggccatga actgtcaccg acgcgcatat
    38341 gaccgcgccc gttgaccagc ggagattcca gcacaaaacg tcggccaatc agctgctgcc
    38401 cgcgctggtt taaatgacta tcgctgtgct tttgttcgcg cacccgccgc gacaaccatt
    38461 tccaccacag ccaggcggcg agcagcgtca ggatggcaaa cattacccct tgccactccc
    38521 aacccagcgg caccagccag accaccaggc cggtaatcac cgctgccacg ccgctccaca
    38581 acaaataacc atttccgccc agcatctcgg ctgccagcag caacccgccg agactgagcc
    38641 agaaaatatg tggatgaacg accattaact ccatcatggc tgagtccgtt tgttggcgct
    38701 gtctttcacc agctcggcaa tcccggcaat cgaccccatc aggctgctgg cctctaatgg
    38761 catcatcact actttgctgt tactggagga accgatctgc tgtaacgctt cggtgtattt
    38821 ttgcgctacg aagtagttca ccgcctgaat atcaccggag gcgatagctt cagacaccat
    38881 tttggtggcg cgggcttctg cttcggcgga acgttcacgc gcttcagcct gtaaaaacgc
    38941 cgactgacgt tcgccttccg ctttcaggat ttgcgactgt ttttcacctt cggctttgag
    39001 gatttccgcc tgacggatcc cttccgcttc aagaatgtaa gcgcgtttgg tacgttccgc
    39061 tttcatctgc gcgttcattg aagagataag ctctgccggt gggcgcacgt cgcgaatttc
    39121 aatacgggtg actttaatcc cccacgggtt ggtggcttca tcgacaatac gcagcaggcg
    39181 tgagttgatg ctgtcgcgct gagagagcat ttcgtcaagt tccattgaac ccaacacggt
    39241 acggatgtta gtcatggtca ggttgatgat cgccagctcc agattgctga cttcataggc
    39301 agcgcgtggc gcgtcaatca cctgaataaa acagactgcg tcgatggtaa cgttggcgtt
    39361 atctttcgag ataacttcct gggaagggat atcgagcact tgctccatca tattgatctt
    39421 gcgaccaatg cgatccataa acggcaccac cagactgagc cccggctgta acgttttggt
    39481 atagcgacca aaacgttcta ccgtccattg atagccctgc ggcacgattt tgacgcccgc
    39541 gccgacaatg accagcgcga caaaaatgag aatcgggata aagataagca tcggaaaaac
    39601 ctcctgttgt accgtccata atcagcaaaa ttgctgcttg attaaacaaa ttatacctga
    39661 ttactgaaag agagttcccc cttattcctg cgaaggataa actgttttta gtaaaaatca
    39721 gaaaaaggga acagcgatgc aggaaaatag tcctttgctt cagctacaaa acgtaggata
    39781 tctggcgggt gatacgaaga ttcttaataa catcaatttt tcgctgcgtg ctggcgaatt
    39841 taagttaatt accggtcctt ctggttgtgg caaaagtacg ctgctaaaaa tagttgcttc
    39901 attgatcagc ccaaccagcg gaacgttact gtttgaaggt gaggatgtca gcacactaaa
    39961 accagaaatc taccgccagc aagtctctta ctgcgcccag acgccgacgc tgtttggcga
    40021 tacggtatac gataatctga tctttccctg gcagatccgt gaccagcagc ctgacccggc
    40081 catttttctc gattttctcg aacgcttcgc tttgccggac agcattttga cgaagaatat
    40141 cgccgagcta tctggtggtg aaaaacaacg catctcattg attcgtaacc tgcaatttat
    40201 gccgaaggtt ttattgctgg atgaaataac cagtgcgctg gatgaaagta ataaacataa
    40261 cgtcaatgag atgatccatc gttatgtgcg cgagcaaaat attgccgtgc tgtgggtgac
    40321 acacgataaa gacgaaatta atcatgcgga taaagtgatt acactgcaac cgcatgccgg
    40381 agaaatgcag gaagcacgct atgaactcgc ataatattac taacgaatca ttagcactgg
    40441 cattaatgct ggtggtggtg gcaatcttaa ttagccataa agaaaaactg gcgctggaga
    40501 aagatattct atggagcgtc gggcgagcga taattcagct gattattgtc ggctatgtgc
    40561 tgaagtatat tttcagcgtg gatgatgcca gcctgacatt attgatggtg ttatttatct
    40621 gctttaatgc ggcgtggaac gcgcaaaaac gcagtaaata tattgctaaa gcttttatct
    40681 catcgtttat tgccattacg gtcggggcgg gaattaccct ggcggtgctg attctctcag
    40741 gggcgattga atttatcccg atgcaggtga tccctgtcgc cgggatgatt gccggtaacg
    40801 ccatggtagc ggtggggttg tgttacaaca atttagggca acgggtcatt agcgaacagc
    40861 aacagattca ggagaaactg agtcttggcg cgacgccgaa gcaggcttca gcgatattga
    40921 ttcgcgacag tattcgcgcg gctttaattc cgacggtcga ttcagcaaaa acggttggct
    40981 tagtgagttt accaggaatg atgtccgggc tgatatttgc cgggattgat ccggtgaagg
    41041 cgattaaata tcagattatg gtgaccttta tgctgctctc aaccgccagc ttgtcgacca
    41101 ttattgcctg ctatttaacc tatcgtaagt tttataattc gcgccaccag ttggtggtga
    41161 ctcaattgaa gaagaaatga tgcgatgccg gatgcgcagc atcgcatccg gcgttgtggt
    41221 tcatgtgccg aacaacggaa tatcaataca acaatgcata cagctggcgg cgatacttcg
    41281 acgccagtgc atcacccgta cccagcgcag cgaggatctc ctggaacgtt ttacgcgtct
    41341 gaccgtctgc ggcggtgaga tctttacgca gatgcccgaa cagcaactcc agcgcctctt
    41401 cattgcgccc aacctgatgc agttgcagcg ccagttgcgt cgccagtgcg gcatcttctg
    41461 gattctccgc cacctgctgt tgcaactgtt gaatttccgg cttatcagcc gcctgcttca
    41521 gcagttcgat ttgcgctacc agcccctggt agcgggtgtc ctgatcctgc aacggaatgg
    41581 ttttcagcac cgcttccgca tcttcagaac ggttcagcgc aatcagcgtt tctgccagca
    41641 gcaggccgat ctccccgttc tgattcgaca actgccaggc gtctttcagc aatggcaggg
    41701 catcggtgta attgccttcc tgcatcagtt gcatcgcctg ctgcgctttc agctcttctt
    41761 cgcgcggcag cactttatcc agcagggcgc ggatcgcctc ttccggttgc ggcccctgga
    41821 agccatctac cggttgcccg ttctggaaca gatacacggt cggaatcgcg cgcagaccaa
    41881 actgggcggc aatcatctgc tccgcgtcgc agtccagctt cgccagaata aattgcccgt
    41941 tgtactgcgc cgcgaggctt tccaggatcg gggttaactg caaacagtgc tggctacgtt
    42001 cagaccaaaa atagaacagc accggagtgg tcatcgactg ttcaagaacc tgttgcaggt
    42061 tagattcgtt aatgttgaca atattttcta cggacatgga gtcgctctct gttgtcgata
    42121 ttttctttga catgggggct taagcgcgcg cttcaactca cccctgcaat attttgtcca
    42181 tcacgcgccc aggcagcagg cgctcgagca ccattactgc ccaggtcacc agcgttaccg
    42241 gatagcgcat cttcggcttc tcgctaataa aagcatggcg cactttgtcg accaccgctt
    42301 ccggtcccaa cgtaaagcgg gcggcgatgc cgggattttc gactggttta tcactttgcg
    42361 tctggttgac gttgtcggtg aagcgagtac gaatgggacc gggttcgatc aggctgactt
    42421 taattccgct gtggcgtagc tccatgcgca gcgcgtctga ccacgcctcc agcgcatatt
    42481 tactggccgc gtaagcgcca cgacccggcg tggagattaa tcccatcacc gatgatgtca
    42541 tcacaatacg cccttcaccg tgcggtaaca tcgcgggtaa caggcgcatg gtgagctggt
    42601 gtgcgccgaa aaagttggcg gaaaactgct gttccatctg cgcacggctg atggtggaaa
    42661 gggggccata catgccgaat ccggcattgt taaagatccc atacagacaa ttatcggtca
    42721 gggcgatcac ctcgtctgct gcgcgattaa cactttctgg tgaatccaga tcgatcaaca
    42781 cgccggtaaa tcccatgttg ttcatacgct caacatcatt cggttttcgg caacctgcca
    42841 gcacatgaaa accctggcgt tttaattcga gcgcgctttc caggccaatt ccactggaac
    42901 atccggtaat taagaccgat ttttgcataa ctttacctgt caggatctcc gttgctttat
    42961 gagtcatgat ttactaaagg ctgcaactgc ttcgccatcc agtcggcaat aaacggctgg
    43021 gcgtcgcggt tgggatgaat accgtcatcc tgcatccatt gtggcttgag gtagacctct
    43081 tccataaaaa agggcagcag cggaacatca aactctttgg cgagtttggg gtaaatggcg
    43141 ctaaaggctt cattataacg gcgaccatag tttgcaggca gacgtatttg cattaacaat
    43201 ggttcagcgt tggcggcttt gacatcctgc aaaatctggc gcagcgtttg ctcggtttgc
    43261 tgtggctgaa aaccacgcaa accatcattg cgcccagttc aaccagcacc caacgcggct
    43321 gatgctgttt gagcagagcc ggaaggcgcg ccagcccttg ttgcgaggtg tcgccgctga
    43381 tgctggcatt aactaccgac gttttactct gccacttatc attcaacaag gcaggccagg
    43441 ccgcgctggc agacattcga tacccggcgc tcaggctatc acccagaatc aataacgtgt
    43501 ccgctgcggc ggcacggaag gttaacagga ccaggaacag gaagggcaaa tgccagcgga
    43561 aaacattgtt gaagttcatc atcttaagaa gtccgtcggt cagggggagc atgaactctc
    43621 catcctcacc ggagttgagc tggttgtcaa acgtggcgag accatcgcac tggtgggcga
    43681 gtcgggatcg ggtaagtcaa ccttgctggc gatcctcgcc gggcttgatg acggcagcag
    43741 tggcgaagtg agtctggtgg gacaaccgct acataatatg gacgaagaag cgcgggcaaa
    43801 gttgcgcgcg aagcacgtcg gctttgtttt tcagtcattt atgttaattc ctacccttaa
    43861 cgcgctggaa aacgtcgagc ttccggctct gctgcgcggt gagagtagcg cggaaagtcg
    43921 taacggggcg aaagcgttgc tcgaacagtt agggctgggt aaacgtctgg atcatcttcc
    43981 ggcacagctt tccggcggtg aacagcaacg agtggcgctg gcacgagcct ttaatggtcg
    44041 acctgatgtg ctgtttgccg acgaacccac cggcaacctt gaccgccaga cgggcgataa
    44101 aattgccgac ctgctgtttt ccctcaaccg tgaacatggc accacgttga ttatggtgac
    44161 ccacgacctg caactggcgg cacgctgcga ccgctgctta cggctggtga acgggcagtt
    44221 gcaggaggaa gcatgattgc acgttggttc tggcgcgaat ggcgttcgcc gtcgctatta
    44281 attgtctggc tggcgctaag cctggcggtg gcctgcgtgc tggcgctggg caatatcagc
    44341 gatcgcatgg agaagggctt aagccagcaa agccgtgagt ttatggcggg cgatcgggcg
    44401 ttgcgcagtt cacgcgaagt gccgcaagcg tggctggagg aagcgcaaaa gcgcggcctg
    44461 aaagtcggca agcagctgac tttcgccaca atgacctttg caggcgacac accgcagctg
    44521 gcgaacgtca aagcggtgga tgatatctac ccgatgtatg gcggtctgca aactaatccc
    44581 cctggcctga aaccgcaggc gggcagcgta ttgctggccc cacgcctgat ggcactgctt
    44641 aacctgaaaa cgggcgacac cattgacgtg ggcgatgcca ccttgcggat tgccggagaa
    44701 gtgattcagg aaccggattc cggttttaac cccttccaga tggctccgcg tctgatgatg
    44761 aatctggcgg atgtcgataa aaccggagcc gtgcagccgg ggagtcgggt cacctggcgt
    44821 tataaattcg gcggcaacga gaaccagctc gacggctatg agaaatggtt gttacctcag
    44881 cttaaacccg aacaacgctg gtacggtctg gaacaggacg aaggcgcgct ggggcgatcg
    44941 atggaacgct cgcaacagtt cctgctgctt tcggcgcttc tgaccttgct gctggcagtg
    45001 gcagcggtgg cggtagcgat gaatcattac tgtcgcagtc gctacgatct ggtggcgatc
    45061 ctcaaaacgc tgggggcagg gcgagcgcaa ctgcgtaagc taatcgtcgg tcagtggttg
    45121 atggtgctga cgctttcagc cgttaccggt ggggccatag gcctgttgtt cgaaaacgtg
    45181 ttgatggtgc tgctcaagcc cgttctgcct gctgcactac cgccagccag cctctggccg
    45241 tggctgtggg cgcttggcac catgacggtc atctcgctgc tggtggggct acgaccatac
    45301 cgcttgttgc tggcaacgca gcctttacgc gtattacgta atgatgtggt cgcgaacgtc
    45361 tggccgctga agttttatct gccgattgtc agtgtggtgg ttgtgctgct gctcgccgga
    45421 ttaatgggtg gcagcatgct gctttgggcg gtgctggcgg gcgcggtagt actggctttg
    45481 ctgtgcggtg tgctgggctg gatgctgctg aatgtacttc gccgcatgac gctgaaatcg
    45541 ctgcctctgc gcctggcggt tagccgcctg ttacgtcagc cgtggtcaac gttaagccag
    45601 ctttcggcat tttcgctctc ctttatgctg ctggcactgc tgctggtgct gcgtggcgat
    45661 ctgctcgacc gctggcagca gcagctaccg ccagaaagcc cgaactactt tttaattaac
    45721 atcgccacag aacaggtagc accgctaaaa gcgttcctcg cggaacatca tatagtcccg
    45781 gaatcatttt atccggtagt gcgggcgcgg ctgacggcga ttaatgataa gccgacagaa
    45841 ggtaatgaag atgaggcgct taatcgcgaa ctcaatctta cctggcaaaa tacgcggccc
    45901 gatcataatc cgattgtcgc cggtaactgg ccgccaaaag ccgatgaagt gtcgatggaa
    45961 gaggggctgg cgaaacgctt aaacgttgcc ctcggtgata ccgtgacttt tatgggcgat
    46021 acccaggagt tccgcgctaa agtgaccagc ctgcgcaaag tggactggga aagtctgcgg
    46081 cctaatttct attttatttt ccctgaaggg gcattagacg ggcaaccgca gagctggctt
    46141 accagtttcc gctgggagaa tggcaacggc atgttgacgc aactcaaccg ccagttcccg
    46201 accattagcc tgttagacat tggcgcgatt ttaaaacagg tcggtcaggt gctggagcag
    46261 gtaagtcggg cgctggaggt gatggtggtg ctggtcaccg cctgcggtat gttgctgttg
    46321 ctggcacagg tgcaggtggg aatgcgccag cgtcatcagg agctggtggt atggcgcaca
    46381 ctcggtgcgg ggaaaaaact gctgcgcacg acattgtggt gtgatgttcg ccatgcttgg
    46441 gtttgtttcc ggcctggtgg ccgcaattgg tgcggaaacg gcactggcag tgttgcaggc
    46501 gaaagtgttt gatttcccgt gggagccaga ctggcgattg tggattgttc tgccgtgcag
    46561 cggtgcgctg ctgctgtcgc ttttcggcgg ctggctgggt gcgcgactgg ttaagggtaa
    46621 ggcgctgttc aggcagtttg tggggtgatg acaggtgtta gattgcctga tgcgctacgc
    46681 ttatcaggcc tacgaagagc gtgcaatata ttgattttgc aagattgtgt aggccggata
    46741 aggcgtttac gccgcatctg gcatgaacag tgtgcatttt ggcaataaat atgaaaccaa
    46801 ctgtcatgtc ggttttttta aaggccgaag gaggtaaacc ttcggcctta gctacgtttc
    46861 ctcagttatt caataaactg acggatcagt tccagcagcg cgattagcgc cttaagaacg
    46921 atgagaactt tatcgagaaa agttttcatc cgttgctccc ttaagggaga aagacaatca
    46981 cagcttaccc ttacgttgcc tataactatg attttcttca tgtgctagat gaggactgtc
    47041 tctctccaaa ttagagcacc agaatcagca ccacctgtat cctgaccagg acttaaaagt
    47101 acttttccgc actgcggtaa tcccttgagg cagtatcact actagcgtcg ggattgtatc
    47161 gccaacatag ctgtttgaat atgtaggcat cctgacttat aattcacttg ctagatgagg
    47221 actgtttaat ttctgcccgg aaccaccaag tcttgggcgg aaaaaataaa aaaaccgact
    47281 gtcatgtcgg tttttttata tctgtattct ggtgcaacca ttttgttctc acgacaatcg
    47341 ccgtttcaga ataccgccca tcactgcgcc ttaacccata ccgccacttc cgcccactca
    47401 ccgcgaaaga caactttttc cgcttttttc tcaagctgat agcgatacat tgggtcgtaa
    47461 tattctttaa gtaacggcac cagccaggcc agatgaccgt cggtgctgcc ggtggtgagt
    47521 tgcgttgtca gcgccgtatc cagctgggca gcgagttcgt tatagcgctg taaccccagc
    47581 cgacgcttaa tcgccgaaag tccgtgatgc aggtattcgc aatattcctg ccagccctgt
    47641 tcgtcgccat aagcgtgggt aaaatcatga tgcatacgca agaaatactc ttcattcagg
    47701 cgttcaagac ggatctcaaa cggatcttct accaccgcaa tcgccgcctg agtcattcgc
    47761 tcgcgcagac attccggcag gtgattcgaa ccgatcatcc ggctttcatc ttccagcacc
    47821 cacaggcgca aatcctgacg ggcgtcggtt tttagcattt cggcagccag caggttttca
    47881 aaactcgcct ggctaagttg tggttgtaac gtgcgaccaa acgccgaacc gcgatgacgc
    47941 gccagccctt ccagatcaac accgtttggc tgttgttgca ctaatagcgt tttaccgctg
    48001 ccggtacaac cgccaatcag cactatcggt ttttgtgcca gttcaatggt cgcctgaatc
    48061 gcggtctggc gcagtgcctt ataaccgcct tccaccagcg gataatcaat ccccgcttca
    48121 tacagccagc gttgcacaat atgtgaacgt tgaccgccac gggcgcagca gagaatacct
    48181 tgcggatttt gcaggcacgc tgcccgccag gcgtacatac gctgctgacg aatttcaccc
    48241 gccaccagtt tatgccccag cgccagcgct gcgtctgagc cttgctgttt atagcaggtg
    48301 ccaacggagg cgcgttcatc gttatccatt aacggcagat tgatagcggc gggcattgcg
    48361 ccttgctcaa actcgatagg ggcgcgaaca tcaataatgg gcgtatcagc aatcagcagg
    48421 gcacgatagt cctgttccgt gtgtctctct tgcatagtta aaagtgaacc tcaaatcagc
    48481 ttgcgcgcta ttttacgcgc caacgcgcaa ggaaacttga tttttaactg cgtggggtgc
    48541 cgaaaatttc taaaaatccg ctgatttccg gcctgtgttg ggtaaacaag gtcacaatat
    48601 cttctaccgc tttgcagctg ccaaatttgc gccatgccag actcaatggc gaaggagggc
    48661 gcatcgttgg gattacccgg ctgaccagtt gttgattatc gatcattgac tggcaaagtg
    48721 attttggcaa aaaaccaatg ccaacacccg ccagatgggc ggcgattttc gtttccatat
    48781 cgggaacaat aatctctttt tgccccggca atcgccaggc gacgcgtttg gttaaggtgc
    48841 cggcgctgtc ttcaatattg accgccggaa agcgacgcaa ctgcgcttct gttagcggct
    48901 cttcaacgtt cgccagcgga tgatccgccg ccatgacaaa tcgccattgc accgatccta
    48961 agggatcaag actaaaggta tttgccagcg cctcagttcc cgtgaccccg atagccagcg
    49021 aaaaaccttc gtacaatagc gagtcccaga cgcccatata gatttgtcgg gagatgtgaa
    49081 actgggtaaa ggggtaacgc tcattcagcc acgccagcaa ctgggcgacg gcctgggggt
    49141 tatagagcag gttgttgatg acaatattca cctggcgttc cacgccatca ttcacctgtt
    49201 gcagctcgct gggcatactt tccagccagc tcagccagtc tctggcctgg gaaagtagat
    49261 gctcgccagc cgctgtcaac gttacgctac gagtcgtacg gaaaaacagc gctactccgg
    49321 tattctcttc cagaagttta atgcgataac tgatcgtcgc cgtggtttta cataatcgtt
    49381 ctgccgcttt tgaaaaactt cctgtttcag caaccgcaat gaaagtccgc aaggtttctg
    49441 gatcgaacat cttcaggtat ccccttttaa atccgcaagt tgcgtgattt tcttatcctc
    49501 tgatttatca gtatttttac atgataatcc tgttcaattt gtggactaaa tctagttttg
    49561 gaaaaacatt ccaacttttg tattaatgtt gttctcttaa ggttttagat tgcctgttat
    49621 tgaaaccaag ctgaccggtc ggcggtggtt gaacggaatt atgttacaag gacaaaaaga
    49681 tgaaacttca ggtattaccg ttaagtcagg aagcctttag tgcttatggc gacgtaatcg
    49741 aaacgcagca acgggatttt ttccatatta acaatggcct ggtggagcgt taccacgatt
    49801 tggcgctggt tgagattctt gagcaagacc gtacgcttat cagcattaac cgcgcgcaac
    49861 cggcgaatct gccgctgacc attcacgaac tcgaacgtca tccgctgggc attcaggcct
    49921 ttatcccaat gaaaggtgaa gtgtttgtgg tggtcgtggc gttaggcgac gacaaaccag
    49981 acctgtcaac gctgcgggcg tttatcacca acggtgaaca gggggtgaat taccatcgta
    50041 acgtctggca tcacccactt ttcgcctggc agcgcgtcac cgattttctg accatcgatc
    50101 gcggcggcag tgacaactgt gatgttgaaa gtattcctga acaggaactc tgttttgcgt
    50161 gacgcctgca accgacttgc ataagataaa ctaattgttc attgtttatg ctcacttgta
    50221 ggtcggagtt aacgtaggta tgacggaagt tagacggcgc ggcaggccag gacaggcggc
    50281 gcctgtggca cagaagggcg cacaggcgtt agagcgggga attgcgattc tgcaatatct
    50341 ggaaaaaagt gggggaagtt cgtcggttag cgatatttct ctcaatctgg atttgccgct
    50401 ctccacgacc tttcgcttgc tgaaggtttt acaagcagcg gattttgtct atcaggacag
    50461 tcaattaggc tggtggcata taggattagg tgtctttaac gtcggtgcgg cgtacatcca
    50521 taaccgcgat gtcctctccg tcgccgggcc gtttatgcgc cgcctgatgt tactttccgg
    50581 cgaaacggtc aatgtcgcga tccgtaacgg caatgaagcg gtattaattg gtcagttaga
    50641 gtgtaaatcg atggtcagga tgtgtgcgcc actgggcagt cgtctgccgc tgcatgcttc
    50701 cggtgcgggc aaagcgctgc tttatccgct ggcggaagag gagttgatga gcatcattct
    50761 gcaaaccggt ttgcagcagt ttacgccaac tacgcttgtg gatatgccca ccttgctgaa
    50821 ggacctggaa caagcgcgtg aactgggcta taccgtagat aaagaagagc atgttgtagg
    50881 tctgaattgc atagcttcag caatttacga tgatgtcggt agagttgttg ccgctatctc
    50941 catctccggg ccttcatcaa gactgacaga agatcgtttt gtcagtcagg gtgagctggt
    51001 cagagacacc gcccgcgata tcagcacggc gttgggactg aaagcacatc catagtgtct
    51061 gtcgcatccc gctctgcgga gcgggttttt ttgacaaaat ttgaaagttg gaaaattttt
    51121 ccaactaata gaggtaggaa taaaatggca aaaatgagag ccgttgacgc ggcaatgtat
    51181 gtgctggaga aagaaggtat caccaccgcc ttcggcgttc cgggagctgc aattaatccg
    51241 ttctactcag cgatgcgtaa gcacggcggt attcgtcaca ttctggcgcg tcatgtggaa
    51301 ggtgtttcgc acatggcgga aggttatacc cgcgcaacgg cagggaatat cggcgtatgt
    51361 ctggggactt ccggtcctgc gggcacggac atgatcaccg cgctctattc cgcttctgct
    51421 gattccattc ctattctgtg cattaccggc caggcaccgc gcgcccgtct gcataaagaa
    51481 gattttcagg ccgtagatat tgaagcaatt gctaaaccgg tcagcaaaat ggcggttaca
    51541 gttcgtgaag cggcgctggt gcctcgcgtg ctgcaacagg catttcacct gatgcgttct
    51601 ggtcgtccgg gtccggtact ggtggattta ccgttcgacg ttcaggttgc ggaaatcgag
    51661 tttgatcctg acatgtacga accgctgccg gtctacaaac ctgctgccag ccgtatgcag
    51721 atcgaaaaag ctgtagaaat gttaatccag gccgaacgtc cggtgattgt tgccgggggc
    51781 ggggtaatca atgctgacgc agctgcactg ttacaacagt ttgctgaact gaccagcgtt
    51841 ccggtgatcc caacgctgat gggctggggc tgtatcccgg acgatcatga actgatggcc
    51901 gggatggtcg gtctgcaaac cgcgcatcgt tacggtaacg caacgttgct ggcgtccgac
    51961 atggtgtttg gtatcggtaa ccgttttgct aaccgtcata ccggttcggt agagaaatac
    52021 accgaagggc gcaaaatcgt tcatattgat attgagccga cgcaaattgg tcgcgtgctg
    52081 tgtccggatc tcggcattgt ctctgatgct aaagcggcgc tgacactgct ggttgaagtg
    52141 gcgcaggaga tgcaaaaagc gggtcgtctg ccgtgtcgta aagaatgggt cgccgactgc
    52201 cagcagcgta aacgcacttt gctgcgcaaa acccacttcg acaacgtgcc ggtgaaaccg
    52261 cagcgcgtgt atgaagagat gaacaaagcc tttggtcgcg atgtttgtta tgtcaccact
    52321 attggtctgt cacaaatcgc tgcggcacaa atgctgcatg tctttaaaga ccgccactgg
    52381 atcaactgtg gtcaggctgg tccgttaggc tggacgattc cggcggcgct ggggggttgt
    52441 gccgctgatc cgaaacgcaa tgtggtggcg atttctggcg actttgactt ccagttcctg
    52501 attgaagagt tagccgttgg cgcgcagttc aacattccgt acatccatgt gctggtgaat
    52561 aacgcctatc tcggcctgat tcgtcagtca caacgcgctt ttgacatgga ctactgcgtg
    52621 caactcgctt tcgagaatat caactccagt gaagtgaatg gctacggtgt tgaccacgta
    52681 aaagtagcgg aaggtttagg ttgtaaagct attcgggtct tcaaaccgga agatattgcg
    52741 ccagcctttg aacaggcgaa agccttaatg gcgcaatatc gggtaccggt agtcgtggaa
    52801 gttattctcg agcgtgtgac caatatttcg atgggcagcg aactggataa cgtcatggaa
    52861 tttgaagata tcgccgataa cgcagcggat gcaccgactg aaacctgctt catgcactat
    52921 gaataaggga gataaatgat gttacgtttc tctgctaatt tatcgatgtt atttggtgaa
    52981 tatgattttc tcgcccgttt tgagaaagct gcgcagtgtg gttttcgcgg cgttgaattt
    53041 atgtttcctt atgactacga cattgaagaa ttaaaacagg tactggcgag taataaactc
    53101 gaacatacgc tgcacaattt accggcaggt gactgggcgg cgggcgagcg cggtattgcc
    53161 tgtattcctg gccgtgaaga agagtttcgg gatggcgtag cagcagcgat t
//
LOCUS       DAJTSI010000009        52575 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000009 DAJTSI010000000
VERSION     DAJTSI010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52575
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.009"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..425
                     /locus_tag="OO327_000473"
     CDS             <1..425
                     /locus_tag="OO327_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6017140.1"
                     /translation="EKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAMLVAQDSRCL
                     LLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYLVALRSGEMI
                     AQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            425..1315
                     /gene="fhuD"
                     /locus_tag="OO327_000474"
     CDS             425..1315
                     /gene="fhuD"
                     /locus_tag="OO327_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR6017141.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            1312..3294
                     /gene="fhuB"
                     /locus_tag="OO327_000475"
     CDS             1312..3294
                     /gene="fhuB"
                     /locus_tag="OO327_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR6017142.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(3329..4609)
                     /gene="hemL"
                     /locus_tag="OO327_000476"
     CDS             complement(3329..4609)
                     /gene="hemL"
                     /locus_tag="OO327_000476"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR6017143.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            4834..6255
                     /gene="clcA"
                     /locus_tag="OO327_000477"
     CDS             4834..6255
                     /gene="clcA"
                     /locus_tag="OO327_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR6017144.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            6279..6344
                     /locus_tag="OO327_000478"
     CDS             6279..6344
                     /locus_tag="OO327_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6017145.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            6337..6681
                     /gene="erpA"
                     /locus_tag="OO327_000479"
     CDS             6337..6681
                     /gene="erpA"
                     /locus_tag="OO327_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR6017146.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(6728..7351)
                     /locus_tag="OO327_000480"
     CDS             complement(6728..7351)
                     /locus_tag="OO327_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR6017147.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(7389..8189)
                     /gene="btuF"
                     /locus_tag="OO327_000481"
     CDS             complement(7389..8189)
                     /gene="btuF"
                     /locus_tag="OO327_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCR6017148.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(8182..8880)
                     /gene="mtnN"
                     /locus_tag="OO327_000482"
     CDS             complement(8182..8880)
                     /gene="mtnN"
                     /locus_tag="OO327_000482"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR6017149.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            8964..10481
                     /gene="dgt"
                     /locus_tag="OO327_000483"
     CDS             8964..10481
                     /gene="dgt"
                     /locus_tag="OO327_000483"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR6017150.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            10611..12035
                     /gene="degP"
                     /locus_tag="OO327_000484"
     CDS             10611..12035
                     /gene="degP"
                     /locus_tag="OO327_000484"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR6017151.1"
                     /translation="MKKNTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            12190..13346
                     /gene="cdaR"
                     /locus_tag="OO327_000485"
                     /pseudo
     CDS             12190..13346
                     /gene="cdaR"
                     /locus_tag="OO327_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(13435..13821)
                     /locus_tag="OO327_000486"
     CDS             complement(13435..13821)
                     /locus_tag="OO327_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR6017152.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(14135..14959)
                     /gene="dapD"
                     /locus_tag="OO327_000487"
     CDS             complement(14135..14959)
                     /gene="dapD"
                     /locus_tag="OO327_000487"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR6017153.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(14990..17662)
                     /gene="glnD"
                     /locus_tag="OO327_000488"
     CDS             complement(14990..17662)
                     /gene="glnD"
                     /locus_tag="OO327_000488"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR6017154.1"
                     /translation="MNALPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(17724..18518)
                     /gene="map"
                     /locus_tag="OO327_000489"
     CDS             complement(17724..18518)
                     /gene="map"
                     /locus_tag="OO327_000489"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR6017155.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            18886..19611
                     /gene="rpsB"
                     /locus_tag="OO327_000490"
     CDS             18886..19611
                     /gene="rpsB"
                     /locus_tag="OO327_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR6017156.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            19869..20720
                     /gene="tsf"
                     /locus_tag="OO327_000491"
     CDS             19869..20720
                     /gene="tsf"
                     /locus_tag="OO327_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR6017157.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            20867..21592
                     /gene="pyrH"
                     /locus_tag="OO327_000492"
     CDS             20867..21592
                     /gene="pyrH"
                     /locus_tag="OO327_000492"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR6017158.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            21884..22441
                     /gene="frr"
                     /locus_tag="OO327_000493"
     CDS             21884..22441
                     /gene="frr"
                     /locus_tag="OO327_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR6017159.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            22533..23729
                     /gene="ispC"
                     /locus_tag="OO327_000494"
     CDS             22533..23729
                     /gene="ispC"
                     /locus_tag="OO327_000494"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR6017160.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            23918..24676
                     /gene="ispU"
                     /locus_tag="OO327_000495"
                     /gene_synonym="uppS"
     CDS             23918..24676
                     /gene="ispU"
                     /locus_tag="OO327_000495"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR6017161.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            24689..25546
                     /gene="cdsA"
                     /locus_tag="OO327_000496"
     CDS             24689..25546
                     /gene="cdsA"
                     /locus_tag="OO327_000496"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR6017162.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            25558..26910
                     /gene="rseP"
                     /locus_tag="OO327_000497"
     CDS             25558..26910
                     /gene="rseP"
                     /locus_tag="OO327_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR6017163.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            26940..29372
                     /gene="bamA"
                     /locus_tag="OO327_000498"
     CDS             26940..29372
                     /gene="bamA"
                     /locus_tag="OO327_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR6017164.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            29494..29979
                     /gene="skp"
                     /locus_tag="OO327_000499"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             29494..29979
                     /gene="skp"
                     /locus_tag="OO327_000499"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR6017165.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            29983..31008
                     /gene="lpxD"
                     /locus_tag="OO327_000500"
     CDS             29983..31008
                     /gene="lpxD"
                     /locus_tag="OO327_000500"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR6017166.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            31113..31568
                     /gene="fabZ"
                     /locus_tag="OO327_000501"
     CDS             31113..31568
                     /gene="fabZ"
                     /locus_tag="OO327_000501"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR6017167.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            31572..32360
                     /gene="lpxA"
                     /locus_tag="OO327_000502"
     CDS             31572..32360
                     /gene="lpxA"
                     /locus_tag="OO327_000502"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR6017168.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            32360..33508
                     /gene="lpxB"
                     /locus_tag="OO327_000503"
     CDS             32360..33508
                     /gene="lpxB"
                     /locus_tag="OO327_000503"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR6017169.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            33505..34101
                     /gene="rnhB"
                     /locus_tag="OO327_000504"
     CDS             33505..34101
                     /gene="rnhB"
                     /locus_tag="OO327_000504"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR6017170.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            34138..37620
                     /gene="dnaE"
                     /locus_tag="OO327_000505"
     CDS             34138..37620
                     /gene="dnaE"
                     /locus_tag="OO327_000505"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR6017171.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            37633..38592
                     /gene="accA"
                     /locus_tag="OO327_000506"
     CDS             37633..38592
                     /gene="accA"
                     /locus_tag="OO327_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR6017172.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            38691..40832
                     /gene="ldcC"
                     /locus_tag="OO327_000507"
     CDS             38691..40832
                     /gene="ldcC"
                     /locus_tag="OO327_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR6017173.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GISTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            40889..41278
                     /locus_tag="OO327_000508"
     CDS             40889..41278
                     /locus_tag="OO327_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6017174.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            41343..42641
                     /gene="tilS"
                     /locus_tag="OO327_000509"
     CDS             41343..42641
                     /gene="tilS"
                     /locus_tag="OO327_000509"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR6017175.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWVRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(42690..42950)
                     /gene="rof"
                     /locus_tag="OO327_000510"
     CDS             complement(42690..42950)
                     /gene="rof"
                     /locus_tag="OO327_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR6017176.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(42937..43137)
                     /locus_tag="OO327_000511"
     CDS             complement(42937..43137)
                     /locus_tag="OO327_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR6017177.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            43303..43848
                     /locus_tag="OO327_000512"
     CDS             43303..43848
                     /locus_tag="OO327_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR6017178.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            43845..44267
                     /gene="arfB"
                     /locus_tag="OO327_000513"
     CDS             43845..44267
                     /gene="arfB"
                     /locus_tag="OO327_000513"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR6017179.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            44281..44991
                     /gene="nlpE"
                     /locus_tag="OO327_000514"
                     /gene_synonym="cutF"
     CDS             44281..44991
                     /gene="nlpE"
                     /locus_tag="OO327_000514"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR6017180.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(45146..45970)
                     /locus_tag="OO327_000515"
     CDS             complement(45146..45970)
                     /locus_tag="OO327_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCR6017181.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(46023..47741)
                     /gene="proS"
                     /locus_tag="OO327_000516"
     CDS             complement(46023..47741)
                     /gene="proS"
                     /locus_tag="OO327_000516"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR6017182.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(47852..48559)
                     /gene="tsaA"
                     /locus_tag="OO327_000517"
     CDS             complement(47852..48559)
                     /gene="tsaA"
                     /locus_tag="OO327_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR6017183.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(48556..48960)
                     /gene="rcsF"
                     /locus_tag="OO327_000518"
     CDS             complement(48556..48960)
                     /gene="rcsF"
                     /locus_tag="OO327_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR6017184.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(49078..49893)
                     /gene="metQ"
                     /locus_tag="OO327_000519"
     CDS             complement(49078..49893)
                     /gene="metQ"
                     /locus_tag="OO327_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR6017185.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(49933..50586)
                     /gene="metI"
                     /locus_tag="OO327_000520"
     CDS             complement(49933..50586)
                     /gene="metI"
                     /locus_tag="OO327_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR6017186.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(50579..51610)
                     /gene="metN"
                     /locus_tag="OO327_000521"
     CDS             complement(50579..51610)
                     /gene="metN"
                     /locus_tag="OO327_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR6017187.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            51798..52373
                     /gene="gmhB"
                     /locus_tag="OO327_000522"
     CDS             51798..52373
                     /gene="gmhB"
                     /locus_tag="OO327_000522"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR6017188.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        12480 a        12942 c        14448 g        12705 t
ORIGIN      
        1 gcgaaaaggt cgaggaagct atctcgctgg ttggcttaaa accgctggcg catcggctgg
       61 tcgatagtct ctctggcggc gaacgtcagc gggcgtggat cgccatgctg gtggcgcagg
      121 atagccgttg tctgttgctc gacgaaccga cttcagcgct ggatatcgcc caccaggttg
      181 atgtgctggc gctggtgcac cgtttaagtc aggaacgtgg cctgacggtc attgccgtgt
      241 tgcacgatat caatatggcg gcacgctact gtgattatct ggtcgccctg cgcagtggtg
      301 aaatgattgc tcagggaacg cctgcggaaa ttatgcgcgg cgaaaccctc gaaatgattt
      361 atggcatccc gatgggtatt ttgccgcatc cggcgggtgc tgcacctgtg agttttgttt
      421 attgatgagc ggcttacctc ttatttcgcg ccgtcgactg ttaacggcga tggcgctttc
      481 tccgttgtta tggcagatga ataccgccca cgcggcggct attgacccca atcgtattgt
      541 ggcgctggag tggttgccgg tggaattact gctggcgctc ggcatcgtgc cttacggcgt
      601 ggcggatacc atcaactatc gcctgtgggt cagcgaacca ccattgccgg actcagtgat
      661 tgacgtcggt ttgcgtacag aacctaacct tgaactgctg accgaaatga aaccgtcgtt
      721 tatggtctgg tcggcaggat atggcccttc atcagaaatg ctggcacgta ttgcgccggg
      781 tcgcggattt aacttcagtg acggcaaaca tccgttggcg atggcgcgta aatcgctgac
      841 ggaaatggca gatttactta acctgcaaag cgcagcggaa acgcatttag cgcaatacga
      901 agactttatc cgcagcatga aaccccgctt tgtgaagcgt ggtgcacgcc cgttactgct
      961 gacgacgctt atcgatccgc gccatatgct ggtcttcggt ccaaacagct tgttccagga
     1021 aattctcgat gagtacggca tcccaaatgc ctggcaaggg gaaaccaact tctggggcag
     1081 taccgccgtc agtatcgatc gtctggcagc gtataaagac gttgatgtgc tctgttttga
     1141 tcacgacaac agcaaagaca tggatgcgct aatggcaacc ccgctgtggc aggccatgcc
     1201 gtttgtccgt acagggcgct ttcagcgcgt acccgcagtc tggttttatg gtgcaacgct
     1261 ttcggcaatg cactttgtgc gtgttctgga taacgccatc ggaggtaaag cgtgagtaaa
     1321 cgaattgcgc ttttcccggc gttattgctg gcgctgttag tgattgtcgc tacggcgctc
     1381 acctggatga acttctcaca ggcgctgccg cgcagtcagt gggcgcaggc cgcctggtcg
     1441 ccggatattg acgtcatcga gcagatgatt tttcactaca gcttgttgcc gcgtctggcg
     1501 atttcgctgc tggtgggcgc gggcctgggg ctggtgggcg tgctgtttca gcaagtactg
     1561 cgtaacccgc tggcggaacc gacgacgttg ggcgttgcaa caggcgcgca actggggatt
     1621 accgtcacca cgctctgggc gatccctagc gcgatggcga gccagtttgc tgcgctggca
     1681 ggggcttgcg ttgttggctt aattgtcttt ggcgtcgcgt gggggaaacg gctgtcgccg
     1741 gtaacgctga ttttcgcggg gttggtagtg agcctttatt gcggcgcaat caatcagtta
     1801 ctggttatct tccatcatga ccagttgcaa agcatgtttc tgtggagcac tggaacgctg
     1861 acgcaaaccg actggggcgg tgttgagcgt ttatggccgc agttgctggg cggtgtgatg
     1921 ctgacgttgc tgctacttcg tccgttaacc ctgatggggc ttgatgatgg cgtggcgcgc
     1981 aatctcgggc tggccttgtc gcttgcgcgt ctggcggcgc tgtcgctggc gattgtcatc
     2041 agtgcgctgc tggtgaacgc tgtggggatt atcggcttta tcgggttgtt cgcgccgctg
     2101 ctggcaaaaa tgctgggggc gcggcgtctg ctgccacgac tgatgctggc gtcgttgatt
     2161 ggtgcgctga tcctctggct ttccgatcaa atcatcctct ggctgactcg cgtgtggatg
     2221 gaagtgtcca ccggttcggt cactgcgttg atcggtgcgc cgctgctact gtggctgctg
     2281 ccgcgtttac gcagcattag cgcgccggat atgaaggtta acgatcgtgt cgcgactgaa
     2341 cgccaacatg tgctggcgtt tgccctcgcg ggcggcgtgc tgttgttgat ggctgtggtg
     2401 gtggcgctgt cgtttggtcg tgatgcgcac ggctggacgt gggcgagcgg ggcgttgctc
     2461 gaggatttaa tgccctggcg ctggccgcga attatggcgg cgctgtttgc gggcgtcatg
     2521 ctggcggtag cgggctgtat tattcagcga ctgaccggca acccgatggc aagcccggaa
     2581 gtgctgggga ttagctccgg cgcggcgttt ggtgtggtgt taatgctgtt tctggtaccg
     2641 ggtaatgcct ttggctggtt gttacccgca ggcagtctcg gggcggcggt gacgctgttg
     2701 atcattatga tcgccgccgg acgcggtgga ttttccccac accgcatgtt actggcgggg
     2761 atggcgttaa gtaccgcgtt caccatgctt ttgatgatgt tgcaggcaag tggtgacccg
     2821 cgaatggcgc aagtgctgac ctggatttcc ggttcgacct acaacgcgac cgatgctcag
     2881 gtctggcgca ccggaattgt gatggtgatt ttgctggcga ttactccgct gtgccgccgc
     2941 tggctgacca ttttaccgct gggtggtgat accgcgcggg cggtagggat ggcgctgacg
     3001 ccgacgcgaa ttgcgctgct gctgttagcg gcttgcctga cggcgaccgc gacgatgact
     3061 attggaccgt tgagttttgt tggtttaatg gcaccgcata ttgcgcggat gatgggcttt
     3121 cgacggacga tgccacacat cgtaatttcg gcgctggtgg gtggtttact gctggtgttc
     3181 gctgactggt gtgggcggat ggtgctgttt ccattccaga tcccggcggg gctgctgtcg
     3241 acctttatcg gtgcgccata ttttatctat ttgctgagaa agcagagccg ttaatagaat
     3301 gcctcgcccg gtgggcgagg caaaattatc acaacttcgc gaacacccga cgtgcagcat
     3361 cgatggtgtt attgatatct tccatgctgt gcgccacaga cataaagcct gcttcaaacg
     3421 ctgacggtgc caggtaaaca ccttcatcca gcatcatatg gaagaaacgc ttaaaacgct
     3481 cgacgtcaca ggccatcaca tcctgatagc acgtcacgga ctcggcgtcg gtaaagaaga
     3541 taccgaacat accaccgacg tggttaacga ccagcggaat tcccgcttct tctgccgctt
     3601 ccagcagacc ttctgccaga cgtgttgtca gctcatccag cgtttcgtga atgcccggct
     3661 gcgcgacttc attcagacag gcgaaacccg ctgccatcgc aatcgggtta ccggaaagcg
     3721 tacccgcctg atagaccgga cctgtcgggg ccagcgcatc cattacatca cgacgaccac
     3781 cgaatgcgcc taccggcatt ccaccgccga tgatttttcc gaggcaggtt aaatctggca
     3841 ctacggcgaa ataatcctgt gcgccagcta gcgctacgcg gaaaccggtc atcacttcat
     3901 cgatgatcag caacgcgcca aattcgtcgc acagcgcacg cagacctggc aggaactctg
     3961 gcagcggcgg aacacagttc atattgcctg ccaccggctc gacgataata caggcaatct
     4021 cttgcgggta ttgctcaaac gcggcgcgta cagaggccag atcgttataa gtacaggtta
     4081 aggtatgttt ggcgaaatct gccggaacgc ccggcgagtt tggctggcct aacgtgagtg
     4141 cgccagaacc ggctttcacc agcaggcagt cagcgtgacc gtggtaacaa ccttcaaatt
     4201 taatgatttt gtcgcgaccg gtaaaaccac gggccaggcg gatggcgctc atcgtcgcct
     4261 cggtgccgga gttcaccatg cgcaccatat ccatggtcgg gaccagttcg gtcaccagtt
     4321 gcgccatttt cacttccatt tcggtcggtg caccaaagct taaaccacgc tcggcggctt
     4381 caatcacggc attgcggatc gccggatggt tatggcccag caccatcggc ccccaggaac
     4441 cgacataatc gatataggct ttgccatcaa catcgtacag ataagcgccg tccgcttttt
     4501 cgataaacag tggagtgccg cccacgccag taaaggcgcg aacaggggag ttcacaccgc
     4561 cagggatcag ctcgcgcgct gcgctgtaaa gattttcaga cttactcatg gagggttcct
     4621 gattcgtaga aaaagtgaat ggctgctatt ccatgttatt cataacaagt taaatactcg
     4681 tcaaacatca ggctgcttgt actggtgcaa tcctgatttc ttagagtata aaagttttgt
     4741 gcatttgaaa cattacgctt tgcaaaggat tttcatggaa cgtgcgagta aaatgccgtc
     4801 atcttatttg tatgaccaat aagtgatcat tggatgaaaa ctgatactcc ctctttagaa
     4861 acaccgcagg ccgcgcgcct gcgacgcaga caactgattc gccaacttct tgagcgcgat
     4921 aaaaccccgt tagccatttt gtttatggcg gcagtcgtcg gcacgcttgt cgggctggct
     4981 gcggttgctt ttgacaaagg tgtcgcctgg ttgcagaacc aacgtatggg ggcgctggta
     5041 catactgctg ataattatcc gctactgtta accgtcgctt ttctctgttc ggcggtgctg
     5101 gcgatgttcg gctacttttt ggtgcgcaaa tacgcgcccg aagcaggggg atcggggatc
     5161 ccggaaattg aaggggcgct ggaagatcaa cgtcctgttc gctggtggcg tgtattgccg
     5221 gtgaaattct ttggcgggct ggggacactc ggcggaggca tggggctagg gcgcgaagga
     5281 ccaaccgtgc agatcggcgg caatatcagt cgcatggtgc ttgatatttt ccgcctgaaa
     5341 ggtgatgaag ctcgccatac gctgctggca accggtgctg ctgcggggct ggctgcggcc
     5401 tttaacgcgc cgctggcggg tattttgttt attatcgaag agatgcgtcc gcagtttcgc
     5461 tatacgttaa tttcgattaa agcggtattt attggcgtca ttatgtcgac cattatgtac
     5521 cggattttta atcatgaagt tgcgttgatt gacgtcggta aactttctga tgcgccgctt
     5581 aatacgctgt ggctttatct gatcctcggt attatttttg gcattttcgg ccctattttt
     5641 aataaatggg tgctggggat gcaggatttg ctgcaccgtg tgcacggcgg caatattacc
     5701 aaatgggtac tgatgggggg ggcgattggc ggtctgtgtg gattgctggg gtttgtggca
     5761 ccagcaacgt cgggcggcgg ttttaacctg attcctatcg ctaccgcggg gaatttcagc
     5821 atgggaatgc tggtgtttat cttcgtcgct cgggtcatta ccaccttact ctgcttctct
     5881 tccggcgcgc cgggcggtat ttttgccccg atgctggcgc tgggtactgt gctgggaacc
     5941 gctttcggaa tggttgccgt tgagctgttt ccgcaatatc acctggaggc ggggacgttt
     6001 gctattgccg gaatgggggc attactggcg gcatctattc gcgcgccgtt aacggggatc
     6061 attctggttc tggagatgac cgataactac cagctcattt tgccaatgat tattaccggt
     6121 cttggcgcaa cactattagc gcaatttacc ggcgggaaac ctctatactc ggcgattctt
     6181 gcgcgcacgc tggcaaaaca ggaagctgag caactggcgc gaagcaaggc cgcatcagcc
     6241 agcgagaata cttgaacgaa ataccagggt attagataat ggcgattatt attgggttag
     6301 aatttgcccg attgccgatg tcgtttggag caaaatatga gtgatgacgt agcactgccg
     6361 ctggagttta ccgacgcagc agccaacaaa gttaaaagcc tgatcgctga cgaagataac
     6421 ccgaatctga aattacgcgt gtatatcacc ggtggcggtt gcagcggctt ccagtatggt
     6481 ttcacctttg atgatcaggt gaacgaaggc gatatgacca tcgaaaaaca gggcgttggc
     6541 ctggtggttg atccgatgag cctgcaatat ctggtcggcg gttccgttga ctacaccgaa
     6601 ggactggaag gttctcgttt catcgtgacc aacccgaacg cgaaaagcac ctgcggttgc
     6661 ggttcttcct ttagcatcta atctgttgtt catgtgccgg ataaactatc cggcacatct
     6721 tctctcttca acgcccattc tcatccagcg caaacgtcgg tagcttaaga tgccaacgaa
     6781 tagccgccag ccgaatcaat agcgtcacga ccatgcccat catactggct gtttccagtg
     6841 gtacggaaaa tgtgtaataa gccgtagcgt ggacaatacc gccgataata caggcagttg
     6901 cgtagatttc tgtacgtaaa atcatgggga tttcgcggac cagtacatca cgaatgatcc
     6961 cgccgccaac gccagtaatg acgcccatac aaaccgcgat taacggaccg gcttccgcat
     7021 taaaggcttt attcacgcta atgccgacaa acaccgccag accaacggcg tccagcaccg
     7081 gcaacatcca ttttggtaag cgtcttggct ggcgcaccag cacgatggtc agcatgctgg
     7141 tgaccatggc aacgaccaga tcggtgggat ctttcaccca aaataccggg ccgtgatcca
     7201 gcgccatgtc gcgaattgtt ccgtcgccta ctgcggtaac cacgcccagt accagaacac
     7261 caaaagggtc catacgcaat tttccggcta acaaaacgcc gtagatggca aataccgctg
     7321 tgccgactat atccagccaa tagacgagca ttgttcaatc cccactgagc acctgaaaag
     7381 gtcaggcact aatctacctg tgaaagcgca ttacagagct gttgtgctgc gaggataata
     7441 cgtgggcttg cacgttcaaa ccagtcactc gtgagaggaa taacgggaat tttgagctgt
     7501 tcaccccagt attgtttgat tttaggaatt tggtccggtc cgcctgtaat gacaatagcc
     7561 tgtggcgagc gtgctaacac ctgttcgcgg ctaacttgcg gccagggaac ccggctgtct
     7621 ttaaagatgt tttctccgcc acaaacttcg agtacctggt tctgaatcga ctcttttcca
     7681 ctggtaaatg gcggattaat gccgaattgc agaaaaacac gttttttagg tttatcagca
     7741 tattgcgctt tcaattgcac gtactgatcc agcagggatt gcgcggcttg ttcggccttg
     7801 tctggttgcg gactccaggg ggccagttga cgtaacgcat tggcaatttg ttcaatgctt
     7861 gtcgcatcga cccacatcac ttttattccc agcgaagcca gctggtcaac ctgccgctcg
     7921 gcattacctc cacgccaggc aatcaccaga tcgggtttca gcgcgacaat gcgttccaga
     7981 ttcatcccct gccaggtgga aacctgctca atcttttgcg cttgtggagg atagtcggaa
     8041 tagctgctga ccccaaccgg cgtgatcccg gcggcaaagg caagttcagt gttggcggga
     8101 gaaagcgtga tgacgcgcgg cgcggcgttg agccacagtg gcgcgagaaa agacagggcg
     8161 accagcgccc tgaacagtga tttagccatg tgccagtttc tgcaccagtg actcaaccat
     8221 caggctggac tgtttagcgg caacagccag gaactcatcg aagctaagat gagactgttg
     8281 atcggccacg tcggagatgg cgcgtacgac aacaaacggg acgttgaaat tgtggcagac
     8341 atgggcgatt gccgtcgctt ccatctctac agcaatggcc tgtgggaagt tgtggcggat
     8401 tttcgccaga ccaacagaac cgttgatgaa agcgtcgccg ctaacaatca ggccacgtac
     8461 agcgttaaga ttcagttcgg caatgcaggc ctcagcggca gcgatcagtt tatcgtcagc
     8521 tttaaagcct gccggacagc ctggtaactg accgtattca taaccaaatg ccgtgacatc
     8581 cgcgtcgtga taacgcgctt cgtccgagac aacgatatcg cccactttca gcgttggtgc
     8641 caggccaccg gcagaaccgg tgttaataat cacatctggc ttgcagtgtt ccaacagcag
     8701 agtggcaccc agcgccgcag cgactttacc gatgcccgat ttcagaagcg caacctcggt
     8761 tccattcagt tggccggtat agatttcgca accgccgaga ctgatagttt gacggttttc
     8821 gattttgtca cgcagcagcg taacttcttc ttccattgca ccaatgatgc cgattttcat
     8881 agatttactc gcgataagcc cgatttgaag gcatagttta ccatgcgctt acggggaagc
     8941 gtatttctca cgcgggagag gacatggcac agattgattt ccgaaaaaaa ataaactggc
     9001 atcgtcgtta ccgttcaccg cagggcgtta aaaccgaaca tgagatcctg cggatcttcg
     9061 agagcgatcg cgggcgtatc atcaactctc cggcaattcg tcgtctgcaa caaaagaccc
     9121 aggtttttcc actggagcgc aatgccgccg tgcgcacgcg tcttacccac tcgatggaag
     9181 tccagcaggt gggacgctac atcgccaaag aaattttaag ccgtctgaaa gagcttaaat
     9241 tgctggaagc atacggcctg gatgaactga ccggcccctt tgaaagcatt gttgagatgt
     9301 catgcctgat gcacgatatc ggcaatccgc cgtttggtca ttttggcgaa gcagcgataa
     9361 atgactggtt tcgccaacgt ttgcacccgg aagatgccga aagccagcct ctgactgacg
     9421 atcgctgcag cgtggcggca ctacgtttac gggacgggga agaaccgctt aacgagctgc
     9481 ggcgcaagat tcgtcaggac ttatgtcatt ttgaggggaa tgcacaaggc attcgtctgg
     9541 tgcatacatt gatgcggatg aatctcactt gggcacaggt tggcggtatt ttaaaatata
     9601 cccgtccggc gtggtggcgt ggcgaaacgc ctgagacaca tcactattta atgaaaaagc
     9661 cgggttatta tctttctgaa gaagcctata ttgcccgctt gcgtaaagaa cttaatttgg
     9721 cgctttacag tcgttttcca ttaacgtgga ttatggaagc tgccgacgac atctcctatt
     9781 gtgtggcaga ccttgaagat gcggtagaga aaagaatatt taccgttgag cagctttatc
     9841 atcatttgca cgaagcgtgg ggccagcatg agaaaggttc gctcttttcg ctggtggttg
     9901 aaaatgcctg ggaaaaatca cgctcaaata gtttaagccg cagtacggaa gatcagtttt
     9961 ttatgtattt acgggtaaac accctaaata aactggtacc ctacgcggca caacgattta
    10021 ttgataatct gcctgcgatt ttcgccggaa cgtttaatca tgcattattg gaagatgcca
    10081 gcgaatgcag cgatcttctt aagctatata aaaatgtcgc tgtaaaacat gtgtttagcc
    10141 atccggatgt cgagcagctt gaattgcagg gctatcgggt cattagcgga ttattagaga
    10201 tttatcgtcc tttattaagc ctgtcgttat cagactttac tgaactggta gaaaaagaac
    10261 gggtgaaacg tttccctatt gagtcgcgct tattccacaa actctcgacg cgccatcggc
    10321 tggcctatgt cgaggctgtc agtaaattac cgtcagattc tcctgagttt ccgctatggg
    10381 aatattatta ccgttgccgc ctgctgcagg attatatcag cggtatgacc gacctctatg
    10441 cgtgggatga ataccgacgt ctgatggccg tagaacaata accagtcttt tgtaaagacg
    10501 aacaataaat ttttaccttt tgcagaaact ttagttcgga acttcgggct ataaaacgaa
    10561 tctgaagaac acagcaattt tgcgttatct gttaatcgag actgaaatac atgaaaaaaa
    10621 acacattagc actgagtgca ctggctctga gtttaggttt ggcgttatct ccgctctctg
    10681 caacggcggc tgagacttct tcagcaacga cagcccagca gatgccaagc cttgcaccga
    10741 tgctcgaaaa ggtgatgcct tcagtggtca gcattaacgt agaaggtagc acaaccgtta
    10801 atacgccgcg tatgccgcgt aatttccagc agttcttcgg tgatgattct ccgttctgcc
    10861 aggaaggttc tccgttccag agctctccgt tctgccaggg gggccagggc ggtaatggcg
    10921 gcggccagca acagaaattc atggcgctgg gttccggcgt catcattgat gccgataaag
    10981 gctatgtcgt caccaacaac cacgttgttg ataacgcgac ggtgattaaa gtccagctga
    11041 gcgatggtcg taagttcgac gcgaagatgg ttggcaaaga tccgcgctct gatatcgcgc
    11101 tgatccaaat ccagaacccg aaaaaactga ccgcaattaa gatggcggat tctgatgcac
    11161 tgcgcgtggg tgattacacc gtagcgattg gtaacccgtt tggtctgggc gagacggtaa
    11221 cttccgggat tgtctctgcg ctggggcgta gcggcctgaa tgctgaaaac tacgaaaact
    11281 tcatccagac cgatgcagcg atcaaccgtg gtaactccgg tggtgcgctg gttaacctga
    11341 acggcgaact gatcggtatc aacaccgcga tcctcgcacc ggacggcggc aacatcggta
    11401 tcggttttgc tatcccgagt aacatggtga aaaacctgac ctcgcagatg gtggaatacg
    11461 gccaggtgaa acgcggtgag ctgggtatta tggggactga gctgaattcc gaactggcga
    11521 aagcgatgaa agttgacgcc cagcgcggtg ctttcgtaag ccaggttctg cctaattcct
    11581 ccgctgcaaa agcgggcatt aaagcgggtg atgtgatcac ctcactgaac ggtaagccga
    11641 tcagcagctt tgccgcactg cgtgctcagg tgggtactat gccggtgggc agcaaactga
    11701 ccctgggctt actgcgcgac ggtaagcagg ttaacgtgaa cctggaactg cagcagagca
    11761 gccagaatca ggttgattcc agctccatct tcaacggcat tgaaggcgct gagatgagca
    11821 acaaaggcaa agatcagggc gtggtagtga acaacgtgaa gacgggcact ccggctgcgc
    11881 agatcggcct gaagaaaggt gatgtgatta ttggtgcgaa ccagcaggca gtgaaaaaca
    11941 tcgctgaact gcgtaaagtt ctcgacagca aaccgtctgt gctggcactg aacattcagc
    12001 gcggcgacag caccatctac ctgttaatgc agtaatctcc ctcaacccct tcctgaatac
    12061 gggaaggggt tctccttaca atctgtgaac ttcgccacaa ctccatacat cttcatcatc
    12121 ctttgggcat ttgcacaatg ccgtacgtta cgtacttcct tatgctaagc cgtgcataac
    12181 ggaggactta tggctggctg gcatcttgat accaaaatgg cgcaggatat cgtggcacgt
    12241 accatgcgca tcatcgatac caatatcaac gtaatggatg cccgcgggcg aattatcggc
    12301 agcggcgatc gtgagcgtat tggtgaattg cacgaaggtg cattgctggt actttcacag
    12361 ggacgagtcg tcgatatcga tgacgcggta gcacgtcatc tgcacggtgt gcggcagggg
    12421 attaatctac cgttacggct ggaaggtgaa attgtcggcg taattggcct gacaggtgaa
    12481 ccagagaatc tgcgtaaata tggcgaactg gtctgcatga cggctgaaat gatgctggaa
    12541 cagtcgcggt tgatgcactt gttggcgcag gatagccgtt tgcgggaaga actggtgatg
    12601 aacctgattc aggcagagga gaatactccc gcacttctga atgggcgcaa cggctgggga
    12661 tcgatctcaa tcaaccgcga gtggtggcta ttgttgaggt cgacagcggt cagcttggcg
    12721 tggacagcgc aatggcggag ttacaacaac tgcaaaacgc gctgactacg cccgagcgta
    12781 ataatctggt ggcgattgtc tcgctaaccg aaatggtggt gttgaaaccg gcgttgaact
    12841 cttttgggcg ctgggatgca gaagatcatc gtaagcgagt tgaacaactg attacccgca
    12901 tgaaagagta cggccagctg cgttttcgcg tttcactggg caactatttt accggtcctg
    12961 gcagtattgc ccgatcctat cgtacggcga aaacgacgat ggtggtgggt aaacagcgga
    13021 tgccagaaag tcgctgctat ttttatcagg atctgatgtt acctgtgtta ctcgacagtt
    13081 tgcgtggcga ctggcaggcc aacgaactgg cgcgaccgct ggcgcggctg aaagcgatgg
    13141 acaataacgg cttgctgcga cgaacgctgg cggcgtggtt tcgccacaat gtgcaaccgc
    13201 tggcaacgtc aaaggcgttg tttattcatc gtaataccct ggagtatcgg cttaatcgta
    13261 tatcggaact gaccgggctt gatttgggca attttgatga caggttgctg ctgtatgtgg
    13321 cgttacaact ggatgaagag cggtagatta tgcgttaagg ttgaggcggc gctggctcat
    13381 tcccctcagc ccgaagggga gaggggaatg cccgagttaa ccgtcaattc gggattattt
    13441 acgcgttagt ttttccagat cggcttcaat ctcgctgatc ttattggtta cgactgactc
    13501 caggtgacgt aagtcgtcga ggatcttacg cttaagatca acttcgctgc ggtcacgctg
    13561 acagatttga tcaagctcat caatgatata ccgtagattc gggctgattt cctggacttc
    13621 tttataaccc tgacccacac catcagcgac gaccgtttta cgctgacgcg gatatttaaa
    13681 cttaacgctc ttggcgaaaa actcgccttt gtctttctgg aaatagattt tcagaatatc
    13741 gttgttggct tcctgccgga ggctgtaacg atcaatttct tcaggattgg taatacccag
    13801 acttttcaga ttgtcgtaca tagcgttacc tcaaaatgag tcagtaaatt gtgcttattt
    13861 tagcatttgg cttggcccgc ccggctcgac tgtgatcgca gaaagccttt ttcttccaga
    13921 cttctatcat ggcgtaaaaa tcaaaaaatt acctgcttta ttgtggcgat attccatggg
    13981 atggattagc acatttatct tcgttttgcg aattacatga cagtccgaaa caagatgaaa
    14041 aagtatttaa aaaaggggga ggaagaatga gtaggacgaa ttattctgag ccagaaagca
    14101 tacgggcagc atcatgctgc ccgtatatgt actcttaatc gatggtacgc agcagttcgt
    14161 taatgccgac tttgccgcga gttttcgcgt caactttctt aacgataacc gcacagtaga
    14221 ggctgtattt gccatctttt gacggcagat tacctgaaac aaccacagac cccgccggaa
    14281 cgcgaccgta gtggatttcg ccggtttcac ggtcgtaaat acgggtgctc tgaccaatgt
    14341 atacgcccat agagatgacg gaaccttctt cgacaatcac cccttcaacc acttcagagc
    14401 gtgcgccaat gaagcagtta tcttcaataa tggtcgggtt agcctgcagc ggctccagca
    14461 cgccgccgat gcccacgcca ccggaaaggt ggacgttttt accaatctgc gcacaagaac
    14521 cgacggtcgc ccaggtatca accatggtgc cttcatcaac atatgcgccg atgttaacgt
    14581 aagacggcat cagcacggtg ttacgggcaa taaacgcacc ctgacgtacc gccgctggtg
    14641 gcacaacgcg gaagccttct ttctggaaac gtgcttcgtc gtagtcggcg aatttcatcg
    14701 gcactttgtc gaagtagcgg ctttctgccc cttcgatcac ctgattatca ttaatacgga
    14761 aagagagcag caccgctttt ttcaaccact gatgcgtcac ccactgaccg tcaatttttt
    14821 ccgctacacg cagtgcgccg gaatccagca gggcgatcac ctgatttacc gcttcgcggg
    14881 taacggtgtc tgcattggct ggcgtgatct cggcacggcg ttcaaaagcg gtttcaataa
    14941 tgttctgtaa ctgctgcatt gttaaactct tttcatatca gtaaacacat caccctttat
    15001 cgtttggatt gagggcctct gtcaaccgct gatgcacttc ctgctgcaac tcgttattaa
    15061 gcgcacgccg gtcagcggtg gcaattatga ataaatcttc tactcgctcg ccaatggttg
    15121 taattcgggc accatgaagc gaaattccca gatcggcaaa aattttcccg actcgcgcca
    15181 gcagtccagg ttggtcgagg gcgatcagtt cgaggaacga tttgcggtcg gtatgggtcg
    15241 gcaaaaacgt tacttcggtt tcaacagtaa aatggcgtaa tttggcgggt tggcgacggg
    15301 gctgcggtgg ctgccagcta ctttgcgtca gcacctgctc cagaccaaac cgaataacct
    15361 catggcgatc tgcggacagc gggctgccat cgggttccag cacgataaag gtatccatcg
    15421 ccataccgtc gcgagtggtg aaaatttgtg cgtcgtgaac acttaaattg cggcggtcta
    15481 attcggcaca gacggcggca aacagatagg ggcggtccgg gctccagata aaaatctcgg
    15541 tgcctccacg cgtagcctgc gggctaagca ataccagcgg tttgcttaaa tcatgctgta
    15601 ataaatggcg ggcatgccag gccagttgat ttgggctatg gcggacaaaa tagttagcac
    15661 gacagcgtga ccaaatttgg tgcagcgcct cttcgtcgat gttatccatg cgcagtagtg
    15721 ccagtgcctg gagttggtga tggcgaaccc gttcgcgcat atccggcgtg ttttgcattc
    15781 cgcgtcgtag ctgcttttcg gtggcaaaat agagttcgcg caacagactt tgcttccagc
    15841 tattccacag tgtttcgttg gtggcgcaaa tgtcagccac tgtcaggcat accagatagc
    15901 gcagacgatt ttccgtttga acttcttcgg caaactgctt gatgacttca gggtcctgaa
    15961 tatcgcggcg ttgtgctgtc accgacatca gcaagtgctg gcgaaccagc caggcaacca
    16021 gctgtgtttc gcgtgagttc agcccgtgga gttctgcaaa atgcactaca tcctgagcac
    16081 cgagaatgga gtggtcgccg ccgcgtcctt tggcgatatc gtgaaacagc gcggcgatga
    16141 aaatcagctc agttgacggc aggcgcggcc agacgtccac acacaacgga tggcgctggc
    16201 gcgtttcttc actggcaaaa ctctccagtt tcagcatcac gcggatagta tgttcatcca
    16261 ccgtgtaggc gtggaacaga tcaaactgca tctgcccgac gatatgcgac cattgcggca
    16321 tatacgcgcc gagcacgcta tggcgatgca ttggcaatag tccgcgccgc accgctccgg
    16381 ggtgacgcag aatgctcaaa aacagttttc gtgcttgcgg aatattacac agcggttgtt
    16441 gcagatggcg acgggcatgg cgtaactggc gcagcgtggt ggagtaaatg ccggtgatcg
    16501 cactgttgcg caccatggtg tagaacatac gcaagatggc ttccggctgg cgcataaata
    16561 gtgtttcatc acgcaggtcg attagcgtac cgcgtagctg aaactcatcg tcgagtggac
    16621 gtggtttttc gtcggcggga agggcgagga tggcttcatc gaacagttgc agcagcatct
    16681 ggttgagttc actgacgcgg cgtgtaacgc ggaagtaatc cttcatcatc cgctcgaccg
    16741 gttcgttgcc ttcaccactg taattcagac gctgagcgac gctaagttgg cgatcgaata
    16801 acagacgatt atcgtaacgg ctgacgacca gatgcagggc aaagcgaata cgccacagta
    16861 tatgcagaca ttcgtttaat tccgcccgct ccgctgaggt taagaagcca aacccgacca
    16921 tttcatccag cgatgttgcg ccaaaatgac ggcgggccac ccattgcaga gtgtgaatat
    16981 cgcgcaagcc gccagggcta cttttgatgt ctggttcaag gttgtagctg gtgccatggt
    17041 aacgctgatg gcgctggttc tgttcttcaa ctttcgctgc gtagaacttg tcggaaggcc
    17101 agaatccttc gctaaaaata tgtttttgca gttcgaggaa gagcgcaaca tcgccaatta
    17161 ataagcggga ttcgattaaa ttggtggcga cggttaaatc cgataaccct tccaacatgc
    17221 actcttcaag cgtgcgcaca ctatgaccga cttccagctt tacatcccag agcagcgtta
    17281 acagctcgcc cactttttgc gcctgatcgt ccgggagctt tttacggctt aaaatcagta
    17341 aatcgatgtc agaaagtgga tgcagctcgc cacgaccgta gccaccgacg gcaaccaatg
    17401 ccaggtcggc aatctggctg aatcccgctt caatccataa tcgttgcagg agctggtcta
    17461 tgaactcggt acgtgcctca atcaactgtt ctgcagagat cccattgtca aaggcatcac
    17521 ccagccaacg ctggaaagta tcgatatggg cttttatccc acagacggtt aattcatcac
    17581 ggggccaggc gcatggattt tgcggttgac cgggcagggt ggagagagcg gtgtttgcgt
    17641 actgttctgg aagggcattc attgtgcgcc acccataaga ttaaattatc gcattaaaaa
    17701 agccggcatt cgccggcttc atcttattcg tcgtgcgaga ttatcgccgg gatggtgtca
    17761 tccttgcgta gcgtcaaaat ttcgcagccg ttatcagtca ccacaatagt atgctcatat
    17821 tgtgcagaca agctgcgatc tttggttttt accgtccagc catctttcat ggtgcggatc
    17881 tcttttttac ccgcgttgac cattggctca atggtgaacg tcatccccgg tttcagcacg
    17941 acgttggttt cacgggagtc atagtgcagc acctgcggtt cttcatggaa gccgcgacca
    18001 ataccgtgtc cgcaatattc acgaacgacg gagaagcctt ctgcttcgac aaatttctga
    18061 atcgccgcac cgatttcgcg cagattaatg cctggtttta ccatgcgtag cgccaggtac
    18121 aggctttctt gcgtgatgcg gcacaggcgt tcgcccatga tggtcggctt accgacgata
    18181 aacattttcg aggtatcgcc gtggaaacca tctttgatta cggtgacatc aatgttaacg
    18241 atatcgccat ctttcagcag cttagcatcg tccgggatac cgtggcacac cacttcatta
    18301 atagagatgc aaacggattt cggatagccg tgatagccga ggcaggcaga aaccgcgtgt
    18361 tgttcattaa caatgtaatc attacagatg cgatccagct cgccggtgct gacgcccggt
    18421 ttaacatacg gttcgatcat ctccagcact tcggcagcca gtcggccagc gacgcgcatt
    18481 ttttcgatat cttctggggt cttgattgag atagccatta attctgtcca tcagcgtcgg
    18541 tgataccgac aatatatatg taagtgccgt caatggtatc acacccgggc aaattgagaa
    18601 tcattctgaa tttcgccaaa cgtgccactg aaggttttct ataatagaaa attcgacgtc
    18661 tgatgctgta cacagcgcca acaattattg gtgtccacga cgtatttgtg gtataaagcg
    18721 cgccggactt ccgatccatt tcgtatacac agactggacg gaagcgacaa tctcactttg
    18781 tgtaacaaca cacacgtatc ggcacatatt ccggggtgcc ctttggggtc ggtaatatgg
    18841 gatacgtgga ggcataaccc caacttttat atagaggttt taatcatggc aactgtttcc
    18901 atgcgcgaca tgctcaaggc tggtgttcac ttcggtcacc agacccgtta ctggaacccg
    18961 aaaatgaagc cgttcatctt cggtgcgcgt aacaaagttc acatcatcaa ccttgagaaa
    19021 actgtaccga tgttcaacga agctctggct gaactgaaca agattgtttc tcgcaaaggt
    19081 aaaatccttt tcgttggtac taaacgcgct gcaagcgaag cggtgaaaga cgctgctctg
    19141 agctgcgacc agttcttcgt gaaccatcgc tggctgggcg gtatgctgac taactggaaa
    19201 accgttcgtc agtccatcaa acgtctgaaa gacctggaaa ctcagtctca ggacggtact
    19261 ttcgacaagc tgaccaagaa agaagcgctg atgcgcactc gtgagctgga gaaactggaa
    19321 aacagcctgg gcggtatcaa agacatgggc ggtctgccgg acgctctgtt tgtaatcgat
    19381 gctgaccacg aacacattgc tatcaaagaa gcaaacaact tgggtattcc ggtatttgct
    19441 atcgttgata ccaactctga tccggacggt gttgacttcg ttatcccggg taacgacgac
    19501 gcaatccgtg ctgtgaccct gtacctgggc gctgttgctg caaccgtacg tgaaggccgt
    19561 tctcaggatc tggcttccca ggcggaagaa agcttcgtag aagctgagta ataaggcttg
    19621 ataactcccc caaaatagtt cgagttgcag aaaggcggca agctcgagaa ttcccgggag
    19681 cttacatcag taagtgaccg ggatgagcga gcgaagacaa cgcatctgcg gcgcgaaata
    19741 tgaaggggga gagcccttat agaccaggta gtacacgttt ggttaggggg cctgcatatg
    19801 gccccctttt tcacttttat atctgtgcgg tttaatgccg ggcagatcac atctccgagg
    19861 attttagaat ggctgaaatt accgcatccc tggtaaaaga gctgcgtgag cgtactggcg
    19921 caggcatgat ggattgcaaa aaagcactga ctgaagctaa cggcgacatc gagctggcaa
    19981 tcgaaaacat gcgtaagtcc ggtgctatta aagcagcgaa aaaagcaggc aacgttgctg
    20041 ctgacggcgt gatcaaaacc aaaatcgacg gcaactacgg catcattctg gaagttaact
    20101 gccagactga cttcgttgca aaagacgctg gtttccaggc gttcgcagac aaagttctgg
    20161 acgcagctgt tgctggcaaa atcactgacg ttgaagttct gaaagcacag ttcgaagaag
    20221 aacgtgttgc gctggtagcg aaaattggtg aaaacatcaa cattcgccgc gttgctgcgc
    20281 tggaaggcga cgttctgggt tcttatcagc acggtgcgcg tatcggcgtt ctggttgctg
    20341 ctaaaggcgc tgacgaagag ctggttaaac acatcgctat gcacgttgct gcaagcaagc
    20401 cagaattcat caaaccagaa gacgtatccg ctgaagtggt agaaaaagaa taccaggtac
    20461 agctggatat cgcgatgcag tctggtaagc cgaaagaaat cgcagagaaa atggttgaag
    20521 gccgcatgaa gaaattcacc ggcgaagttt ctctgaccgg tcagccgttc gttatggaac
    20581 caagcaaaac tgttggtcag ctgctgaaag agcataacgc tgaagtgact ggcttcatcc
    20641 gtttcgaagt gggtgaaggc atcgagaaag ttgagactga ctttgcagca gaagttgctg
    20701 cgatgtccaa gcagtcttaa ttatcaaaaa ggagccgcct gagggcggct tctttttgtg
    20761 cccatcttgt aaattcagct aacccttgtg gggctgcgct gaaaagcgac gtacaatgtc
    20821 gctagtatta attcatttca atcgttgaca gtctcaggaa agaaacatgg ctaccaatgc
    20881 aaaacccgtc tataaacgca ttctgcttaa gttgagtggc gaagctctgc agggcactga
    20941 aggcttcggt attgatgcaa gcatactgga tcgtatggct caggaaatca aagaactggt
    21001 tgaactgggt attcaggttg gtgtggtgat tggtgggggt aacctgttcc gtggcgctgg
    21061 tctggcgaaa gcgggtatga accgcgttgt gggcgaccac atggggatgc tggcgaccgt
    21121 aatgaacggc ttggcaatgc gtgatgcact gcaccgcgcc tatgtgaacg ctcgtctgat
    21181 gtccgctatt ccattgaatg gcgtgtgcga cagctacagc tgggcagaag ctatcagcct
    21241 gttgcgcaac aaccgtgtgg tgatcctctc cgccggtaca ggtaacccgt tctttaccac
    21301 cgactcagca gcttgcctgc gtggtatcga aattgaagcc gatgtggtgc tgaaagcaac
    21361 caaagttgac ggcgtgttta ccgctgatcc ggcgaaagat ccaaccgcaa ccatgtacga
    21421 gcaactgact tacagcgaag tgctggaaaa agagctgaaa gtcatggacc tggcggcctt
    21481 cacgctggct cgtgaccata aattaccgat tcgtgttttc aatatgaaca aaccgggtgc
    21541 gctgcgccgt gtggtaatgg gtgaaaaaga agggacttta atcacggaat aattcccgtg
    21601 atggataaat aagggtaaga ttccgcgtaa gtatcgcggg ggcgtaagtc tggttataag
    21661 gcgttattgt tgcaggcagt ttggtcacgg ccagcgcgca gcaaccggag cgtacaaaag
    21721 tacgtgagga tggcgagcac tgctcggggc caaaatggca aataaaatag cctaataatc
    21781 cagacgatta cccgtaatat gtttaatcag ggctatactt agcacacttc cactgtgtgt
    21841 gactgtctgg tctgactgag acaagttttc aaggattcgt aacgtgatta gcgatatcag
    21901 aaaagatgct gaagtacgca tggacaaatg cgtagaagcg ttcaaaaccc aaatcagcaa
    21961 aatacgcacg ggtcgtgctt ctcccagcct gctggatggc attgtcgtgg aatattacgg
    22021 cacgccgacg ccgctgcgtc agctggcaag cgtaacggta gaagattccc gtactctgaa
    22081 aatcaacgtg tttgatcgtt caatgtctcc ggccgttgaa aaagcgatta tggcctccga
    22141 tctcggcctg aacccgaact ctgcgggtag cgacatccgt gttccgctgc cgccgctgac
    22201 ggaagaacgt cgtaaagatc tgaccaaaat cgttcgtggt gaagcagaac aagcgcgtgt
    22261 tgcagtacgt aatgtgcgtc gtgacgcgaa cgacaaagtg aaagcactgt tgaaagataa
    22321 agagatcagc gaagacgacg atcgccgttc tcaggacgat gtacagaaac tgactgatgc
    22381 tgcaatcaag aaaattgaag cggcgctggc agacaaagaa gcagaactga tgcagttctg
    22441 atttcttgaa cgacaaaaac gccgctcagt agatccttgc ggatcggctg gcggcgtttt
    22501 gctttttatt ctgtctcaac tctggatgtt tcatgaagca actcaccatt ctgggctcga
    22561 ccggctcgat tggttgcagc acgctggacg tggtgcgcca taatcccgaa cacttccgcg
    22621 tagttgcgct ggtggcaggc aaaaatgtca ctcgcatggt agaacagtgc ctggaattct
    22681 ctccccgcta tgccgtaatg gacgatgaag cgagtgcgaa acttcttaaa acgatgctac
    22741 agcaacaggg gagccgcacc gaagtcttaa gtgggcaaca agccgcttgc gatatggcag
    22801 cgcttgagga ggttgatcag gtgatggcag ccattgttgg tgctgctggg ctgttaccta
    22861 cgcttgctgc gatccgcgcg ggtaaaacca ttttgctggc caataaagaa tcactggtta
    22921 cctgcggacg tctgtttatg gacgccgtaa agcagagcaa agcgcaattg ttaccggtcg
    22981 atagcgaaca taacgctatt tttcagagtt taccgcaacc tatccagcat aatctgggat
    23041 acgctgacct tgagcaaaat ggcgtggtgt ccattttact taccgggtct ggtggccctt
    23101 tccgtgagac gccattgcgc gatttggcaa caatgacgcc cgatcaagct tgccgtcatc
    23161 cgaactggtc gatgggacgt aaaatttctg tcgattcggc taccatgatg aacaaaggtc
    23221 tggaatacat tgaagcgcgt tggctgttta acgccagcgc cagccagatg gaagtgctga
    23281 ttcacccgca gtcagtgatt cactcaatgg tgcgctatca ggacggcagt gttctggcgc
    23341 agctggggga accggatatg cgtacgccaa ttgcccacac catggcatgg ccgaatcgcg
    23401 tgaactctgg cgtgaagccg ctcgattttt gcaaactaag tgcgttgaca tttgccgcac
    23461 cggattatga gcgttatcca tgcctgaaac tggcgatgga ggcgttcgaa caaggccagg
    23521 cagcgacgac agcattgaat gccgcaaacg aaatcaccgt tgctgctttt cttgcgcaac
    23581 aaatccgctt tacggatatc gctgcgttga atttatccgt actggaaaaa atggatatgc
    23641 gcgaaccaca atgtgtggac gatgtgttat ctgttgatgc gaacgcgagt gaagtcgcca
    23701 gaaaagaggt gatgcgtctc gcaagctgag gataatccgg ctacagagag tcgcgctatt
    23761 tgttagcgta gggcttcagt gatatagtct gcgccatctg atcgtaagta gttggcttta
    23821 taaggtcagg tatgccgtgg ttttacacgg cttttttttg tataggcttc agtattcctg
    23881 agtaccgtaa accctgtcag ggaataaaaa acgcgtgatg ttgtctgcta ctcaaccact
    23941 tagcgaaaaa ttgccagcgc atggctgccg tcatgttgcg atcattatgg acggcaatgg
    24001 tcgctgggca aaaaagcaag ggaagattcg tgcctttggg cataaagccg gggcaaaatc
    24061 cgtccgccgg gctgtctctt ttgcggccaa caacggtatt gaggcgttaa cgctgtatgc
    24121 ctttagtagt gaaaactgga accgaccagc gcaggaagtc agtgcgttaa tggaactgtt
    24181 tgtgtgggcg ctcgatagcg aagtaaaaag tctgcaccga cataacgtgc gtctgcgtat
    24241 tattggcgat accagtcgtt ttaactcgcg tttgcaagaa cgtattcgta aatctgaagc
    24301 gctaacagcc ggaaataccg gtctgacgct gaatattgcg gcgaactacg gtggacgttg
    24361 ggatatagtc cagggagtca ggcaactggc tgaaaaggtg cagcaaggaa acctgcaacc
    24421 agatcagata gatgaagaga tgttaaacca gcatgtctgt atgcatgaac tggcccctgt
    24481 agatttagta attaggactg ggggagagca tcgcattagt aactttttgc tttggcaaat
    24541 tgcctatgcc gaactttact ttacagatgt tctctggccc gatttcgatg aacaagactt
    24601 tgaaggggcg ttaaatgcct ttgctaatcg agagcgtcgt ttcggcggca ccgagcccgg
    24661 tgatgaaaca gcctgatggg ggtcgctttt gctgaagtat cgcctgatat ctgcttttgt
    24721 gttaataccc gtcgtcatcg cggcgttgtt tctgttgccg ccggtggggt tcgccattgt
    24781 aacgctggtg gtctgcatgc tggcagcgtg ggaatgggga cagcttagcg gttttaccac
    24841 tcgttcgcag cgagtatggt tggcggtgtt atgcgggtta ttgttggcgc tgatgctttt
    24901 tctgttgccg gaatatcacc gaaatattca tcaaccgctg gttgaaatct cactttgggc
    24961 ttcgttgggt tggtggattg tcgcgctatt gctggtgctg ttttacccag gttccgcagc
    25021 aatctggcgt aactctaaaa cattgcgcct tatttttggc gtgctaacca ttgttccctt
    25081 cttctggggc atgctggcgt tacgggcctg gcactatgac gagaatcatt acagtggcgc
    25141 aatatggctg ctctatgtca tgatcctggt atggggcgct gactccggcg catatatgtt
    25201 tggcaaattg tttggtaaac ataagctggc accgaaggtt tctccgggta aaacctggca
    25261 aggctttata ggtggactcg ctactgcagc ggtaatctca tggggttatg gcatgtgggc
    25321 gaatctcgac gtcgctcccg tcaccttact catttgctct attgtcgcag cgttagcctc
    25381 agtgctcggc gatctgaccg agagtatgtt taagcgtgaa gcaggaatta aggacagcgg
    25441 tcatttaatt ccaggacacg gtggtatttt agatcgtatt gatagcctga cggctgcggt
    25501 accggtcttt gcttgcttgt tgttactggt attcaggacg ctttaacgga aggtaatatg
    25561 ctgagttttc tctgggattt ggcttcgttc atcgttgcac tgggtgtact tatcaccgtg
    25621 catgaatttg gtcatttctg ggttgcccgg cgttgtggtg ttcgcgttga gcgtttctca
    25681 atagggtttg gtaaggcgct ctggcggcga actgataagc tcggcaccga atatgttatc
    25741 gccctgatcc cgttgggcgg ttatgtcaaa atgctggatg agcgcgcaga accggtcgtt
    25801 ccggaactcc gccaccatgc cttcaataat aaatctgtcg gccaacgagc ggcgattatt
    25861 gccgcaggtc cggttgcaaa cttcattttt gctatctttg cctactggct ggtttttatt
    25921 attggtgtgc ctggcgtacg tccggtggtt ggcgaaatag cagccaattc gatagctgcg
    25981 gaagcacaaa ttgcaccagg tacggaacta aaagccgtag atggtatcga aacgcctgat
    26041 tgggatgccg tgcgtttgca gttggtcgat aaaattggcg atgaaagcac caccattaca
    26101 gtagcgccat ttgacagcga ccaacggcgg gatgtaaagc tcgatttacg tcactgggcg
    26161 tttgagcctg ataaagaaga tccggtatct tcgctgggga ttcgtcctcg tgggccgcaa
    26221 attgaacctg tactggaaaa tgtgcagcca aactcggcgg caagcaaggc aggtttgcaa
    26281 gcaggcgaca ggatcgttaa agtcgatggt cagcccttaa cgcagtgggt gacctttgtg
    26341 atgcttgtcc gggataaccc gggtaaatcc ttagcgttag aaatcgaaag gcaggggagt
    26401 cccttgtctt tgacattaat cccggagagt aaaccgggta atggtaaagc gattggtttt
    26461 gtcggtattg agccgaaagt cattcctttg ccagatgagt ataaagttgt acgccagtat
    26521 gggccgttca acgccatcgt cgaagccacg gacaaaacgt ggcagctgat gaagctgacg
    26581 gtcagtatgc tgggaaaatt gatcaccggt gatgtgaaac tgaacaacct cagtgggccg
    26641 atctctatcg ccaagggggc tgggatgaca gcggaactcg gggtagttta ttacctgccg
    26701 tttcttgcgc ttattagcgt gaacttaggg ataattaacc tgtttccgtt gcccgtactt
    26761 gacggggggc atctgctgtt ccttgcgatc gaaaagatca agggcggacc ggtatccgag
    26821 cgggttcaag acttttgtta tcgcattggc tcgattctgc tggtgctgtt aatggggctt
    26881 gcacttttca atgatttctc tcggttatga gagttagtta ggaagaacgc ataataacga
    26941 tggcgatgaa aaagttgctc atagcgtcgc tgctgtttag cagcgccacc gtatacggtg
    27001 ctgaagggtt cgtagtgaaa gatattcatt tcgaaggcct tcagcgtgtc gccgttggtg
    27061 cggccctcct cagtatgccg gtgcgtacag gcgacacggt taatgatgaa gatatcagta
    27121 ataccattcg cgctctgttt gctaccggca actttgagga tgttcgcgtc cttcgtgatg
    27181 gtgataccct tctggttcag gtaaaagaac gtccgaccat tgccagcatt actttctccg
    27241 gtaacaaatc ggtgaaagat gacatgctga agcaaaacct cgaggcttct ggtgtgcgtg
    27301 tgggcgaatc cctcgatcgc accaccattg ccgatatcga gaaaggtctg gaagacttct
    27361 actacagcgt cggtaaatat agcgccagcg ttaaagctgt cgtgaccccg ctgccgcgca
    27421 accgtgttga cctaaaactg gtgttccagg aaggtgtgtc agctgaaatc cagcaaatta
    27481 acattgttgg taaccatgct ttcaccaccg acgaactgat ctctcatttc caactgcgtg
    27541 acgaagtgcc gtggtggaat gtggtaggcg atcgtaaata ccagaaacag aaactggcgg
    27601 gcgatcttga aaccctgcgc agctactatc tggaccgcgg ttatgcccgt ttcaacatcg
    27661 actctaccca ggtcagtctg acgccagata aaaaaggtat ttacgtcacg gtgaacatca
    27721 ccgaaggcga tcagtacaag ctttctggcg ttgaagtgag cggcaacctt gccgggcact
    27781 ccgctgaaat tgagcaactg actaagatcg agccgggcga gctgtataac ggcactaaag
    27841 tgaccaaaat ggaagatgac attaaaaagc ttctcggtcg ctatggttat gcctatccgc
    27901 gcgtacagtc gatgcctgaa attaacgatg ccgacaaaac cgttaaatta cgcgtgaacg
    27961 ttgatgcggg taaccgtttc tacgtgcgta agatccgttt tgaaggtaac gatacctcga
    28021 aagatgccgt cctgcgtcgc gaaatgcgtc agatggaagg tgcatggttg gggagcgatc
    28081 tggtcgatca gggtaaggag cgtctgaatc gtctgggctt ctttgaaact gtcgataccg
    28141 atacccaacg tgttccgggt agcccggacc aggttgatgt agtctacaag gtgaaagaac
    28201 gtaacactgg tagcttcaac ttcgggattg gttacggtac tgaaagtggc gtgagcttcc
    28261 aggctggcgt gcaacaggat aactggttag gtacaggtta tgctgttggt atcaacggga
    28321 ccaaaaacga ttaccagacc tatgctgaac tgtcggtaac caacccgtac ttcaccgtag
    28381 atggcgtaag cctcggtggt cgtctcttct ataatgactt ccaggcagat gacgccgacc
    28441 tgtccgacta taccaacaag agttatggta cagacgtgac gttgggcttc ccgattaacg
    28501 aatataactc gctgcgtgca ggtctgggtt atgtacataa ctccctgtcc aacatgcagc
    28561 ctcaggttgc gatgtggcgt tatctgtact ctatgggtga acatccgagc acctctgatc
    28621 aggataacag cttcaaaacg gacgacttca cgttcaacta tggttggacc tataacaagc
    28681 ttgaccgtgg ttacttcccg acagatggtt cacgtgtcaa cctgaccggt aaagtgacca
    28741 ttcctggatc ggataacgaa tactacaaag tgacgttaga cacggcgact tatgtgccga
    28801 tcgatgacga tcacaaatgg gttgttctgg gtcgtacccg ctggggttat ggtgatggtt
    28861 taggcggcaa agagatgccg ttctacgaga acttctatgc cggtggttcc agcaccgtgc
    28921 gtggcttcca gtccaatacc attggtccga aagcagttta cttcccgcat caggccagta
    28981 attatgatcc ggactatgat tacgaatgtg cgactcagga cggcgcgaaa gacctgtgta
    29041 aatcggatga tgctgtaggc ggtaacgcca tggcggttgc cagcctcgag ttcatcaccc
    29101 cgacgccgtt tattagcgat aagtatgcta actcggttcg tacttcattc ttctgggata
    29161 tgggtaccgt ttgggataca aactgggatt ccagccaata ttctggatat ccggactata
    29221 gtgatccaag caatatccgt atgtctgcgg gtatcgcatt acaatggatg tccccattgg
    29281 ggccgttggt gttctcctac gcccagccgt tcaaaaagta cgatggagac aaggcagaac
    29341 agttccagtt taacatcggt aaaacctggt aagtgttctc cacaaaggaa tgtagtggta
    29401 gtgtagcgat gactttaggc gatcaatata agatcgccgg gccacgcaaa gaactgcacc
    29461 ctccggtgca aatgggatgg taaggagttt attgtgaaaa agtggttatt agctgcaggt
    29521 cttggtttag cactggcaac ttctgctcag gcggctgaca aaattgcaat cgtcaatatg
    29581 ggcagcctgt tccagcaggt agcgcagaaa accggtgttt ctaacacgct ggaaaatgag
    29641 ttcaaaggcc gtgccagcga actgcagcgt atggaaaccg atctgcaggc taaaatgaaa
    29701 aaactgcagt ccatgaaagc gggcagcgat cgcaccaaac tggaaaaaga cgtgatggct
    29761 cagcgccaga cttttgctca gaaagcgcag gcttttgagc aggatcgcgc acgtcgttcc
    29821 aacgaagaac gcggcaaact ggttactcgt atccagactg ctgtgaaatc cgttgccaac
    29881 agccaggata tcgatctggt tgttgatgca aacgccgttg cttacaacag cagcgatgta
    29941 aaagacatca ctgccgacgt actgaaacag gttaaataag taatgccttc aattcgactg
    30001 gctgatttag cgcagcagtt ggatgcagaa ctacacggtg atggcgatat cgtcatcacc
    30061 ggcgttgcgt ccatgcaatc tgcacaaaca ggtcacatta cgttcatggt taacccaaaa
    30121 taccgtgagc atttaggctt gtgccaggcg tccgcggttg tcatgaccca ggacgatctt
    30181 cctttcgcga aaagtgccgc gctggtagtg aagaatccct acctgactta cgcgcgcatg
    30241 gcgcaaattt tagataccac gccgcagccc gcgcagaaca ttgcacccag tgcggtgatc
    30301 gacgcgacgg cgaagctggg taacaacgta tcgattggcg ctaacgcggt gattgagtcc
    30361 ggcgttgaac tgggcgataa cgtgattatc ggtgccggtt gcttcgtagg taaaaacagc
    30421 aaaatcggtg caggttcgcg tctctgggcg aacgtaacca tttaccatga gatccagatc
    30481 ggtcagaatt gcctgatcca gtccggaaca gtggtaggcg cagacggctt tggttatgcc
    30541 aacgatcgtg gtaactgggt gaagatccca cagattggtc gcgtaattat tggcgatcgc
    30601 gtggagatcg gtgcctgcac aaccatcgat cgcggcgcgc tggatgacac tgttattggc
    30661 aatggcgtga tcattgataa ccagtgccag attgcacata acgtcgtgat tggcgacaat
    30721 acggcggttg ccggtggcgt cattatggcg ggcagcctga aaattggtcg ttactgcatg
    30781 atcggcggag ccagcgtaat caacgggcat atggaaatat gcgacaaagt gacggttacg
    30841 ggcatgggta tggtgatgcg tcccatcact gaaccaggcg tctattcctc aggcattccg
    30901 ctgcaaccca acaaagtctg gcgcaaaacc gctgcactgg tgatgaacat tgatgacatg
    30961 agcaagcgtc tgaaatcgct tgagcgcaag gttaatcaac aagactaacg ttccatcttt
    31021 tgttcgccaa actttacggc ctgtctcatt cttacgattg cggcaggccg tgttattatt
    31081 gtcgtttctt atattttgac aggaagagta tcttgactac taacactcat actctgcaga
    31141 ttgaagagat tttagaactt ctgccgcacc gtttcccgtt cttactggtg gatcgcgtgc
    31201 tggattttga agaaggtcgt tttctgcgcg cagtaaaaaa tgtctctgtc aatgagccat
    31261 tcttccaggg ccatttccct ggaaaaccga ttttcccggg tgtgctgatt ctggaagcaa
    31321 tggcacaggc aacaggtatt ctggcgttta aaagcgtagg aaaactggaa ccgggtgagc
    31381 tgtactactt cgctggtatt gacgaagcgc gcttcaagcg cccggtcgtg cctggcgatc
    31441 aaatgatcat ggaagtcact ttcgaaaaaa cgcgccgcgg cctgacccgt tttaaagggg
    31501 ttgctctggt cgatggtaaa gtagtttgcg aagcaacgat gatgtgtgct cgtagccggg
    31561 aggcctgata cgtgattgat aaatccgcct ttgtgcatcc aaccgccatt gtggaagagg
    31621 gcgcgtcaat tggcgcgaac gcacacattg gtcctttttg tatcgttgga ccccatgtcg
    31681 aaattggtga gggtaccgta ctgaaatctc acgttgtcgt gaatggtcat actaaaattg
    31741 gccgcgataa tgagatttat cagtttgcct ccatcggcga agttaaccag gatttgaaat
    31801 atgctggcga accgacccgt gtggaaatcg gcgatcgtaa ccgcattcgc gaaagcgtca
    31861 ccattcatcg tggcacagtc cagggcggtg gattgacgaa ggtgggcagc gacaacttac
    31921 tgatgatcaa cgcgcacatt gcgcacgatt gtacggtagg taaccgctgt attctcgcca
    31981 acaacgcaac gctggcgggt cacgtatcgg ttgacgactt cgcgatcatc ggcggcatga
    32041 ccgcagtcca tcagttctgc atcattggtg cgcatgtgat ggttggcggc tgctccggtg
    32101 tggcgcagga cgtccctcct tatgttattg cgcagggtaa ccacgcaacg ccgttcggtg
    32161 tcaatatcga agggctgaag cgccgcggat tcagccgtga ggcgattacc gctatccgca
    32221 atgcgtataa gctaatttat cgtagcggta aaacgctcga tgaagtgaaa ccggaaattg
    32281 ctgaactggc ggaaacatat ccggaagtga aagcctttac cgatttcttt gcacgctcaa
    32341 cgcgcggtct gattcgttaa tgactgacca gcgtccatta acgattgccc tggtcgccgg
    32401 agaaacctcc ggcgatatcc tgggggccgg tttaatccgc gctctgaaag aacgtgtgcc
    32461 caacgcccgc tttgttggtg ttgccgggcc acgaatgcag gctgaaggct gcgaagcctg
    32521 gtacgaaatg gaagaactgg cggtgatggg cattgttgaa gtgctcggtc gtctgcgtcg
    32581 cttactgcat attcgtgccg atctgacaaa gcgttttggc gaactgaagc cagatgtttt
    32641 tgttggtatt gatgcgcccg acttcaatat tactcttgaa ggtaacctca aaaagcaggg
    32701 tatcaaaacc attcattacg tcagtccatc cgtctgggcg tggcgacaga aacgcgtttt
    32761 caaaataggc agagccaccg atctggtgct cgcatttctg cctttcgaaa aagcgtttta
    32821 tgacaaatac aatgtaccgt gccgctttat cggtcatacc atggctgatg ccatgccgtt
    32881 agatccagat aaaaatggtg cccgtgatgt gctggggatc ccttacgatg cccactgtct
    32941 ggcattgttg ccgggcagcc gtggtgcaga agtcgaaatg cttagtgccg atttcttgaa
    33001 aactgcccag cttttgcgcc agacatatcc ggatctcgaa atcgtggtgc cgctggtgaa
    33061 tgccaaacgc cgcgagcagt ttgaacgcat caaagctgaa gtcgcgccag acctgtcggt
    33121 tcatttgctg gatggaatgg gccgtgaggc gatggtcgcc agcgatgcgg cactactggc
    33181 gtcggggacg gcagccctgg agtgtatgct ggcgaaatgc ccgatggtgg tgggatatcg
    33241 catgaagcct tttaccttct ggttggcgaa gcggctggtg aaaactgatt atgtctcgct
    33301 gccaaatctg ctggcgggca gagagttagt caaagagtta ttgcaggaag agtgtgagcc
    33361 gcaaaaactg gctgcggcgc tgttaccgct gttggcgaac gggaaaacca gccacgcgat
    33421 gcacgatacc ttccgtgaac tgcatcagca gatccgctgc aatgccgatg agcaggcggc
    33481 acaagccgtt ctggagttag cacaatgatc gaatttgttt atccgcacac gcagctggtt
    33541 gcgggtgtgg atgaagtcgg acgcgggccg ttagttggcg cggtcgtcac cgctgcggtg
    33601 atccttgacc cggcgcgccc gattgccggg ctgaatgatt ccaaaaagct gagcgaaaaa
    33661 cgccgtctgg cgctctatga agagatcaaa gagaaagcgt tgagctggag tctgggccgc
    33721 gcggaacccc acgaaatcga cgagctgaac attcttcatg cgaccatgct ggcgatgcag
    33781 cgtgccgtcg ctgggctgca tattgcgccg gaatatgtgt tgattgatgg taaccgctgc
    33841 ccgaaattac cgatgcctgc gatggctgtg gtgaaagggg atagccgcgt accagaaatc
    33901 agtgccgcgt ctatcctggc gaaagtgacg cgtgacgccg aaatggcggc gctggatatt
    33961 gttttcccgc aatatggttt tgcccaacat aaagggtatc caaccgcttt tcatctggaa
    34021 aaactggctg aatatggcgc gactgaacat catcgtcgca gctttgggcc tgtcaaacgc
    34081 gcactgggac ttgcgtcctg attcttgtgt cgagattaag taaaccggaa tctgaagatg
    34141 tctgaaccac gtttcgtaca cctgcgggtg cacagcgact actcgatgat cgatggcttg
    34201 gccaaaaccg caccgctggt aaaaaaggcg gcggcgttgg gtatgccagc actggcgatc
    34261 accgatttca ccaacctttg cggtctggtg aagttctacg gagcgggaca tggcgcaggg
    34321 attaagccta tcgtcggggc agattttaat gtccagtgcg acctgctggg tgatgagtta
    34381 acccacctga cggtactggc ggcgaacaat accggctatc agaatctggc gttgctgatc
    34441 tcaaaagcgt atcagcgcgg gtacggtgcc gccgggccga tcatcgatcg cgactggctt
    34501 atcgaattaa acgaagggtt gatccttctt tccggcgggc gcatgggcga cgtcggacgc
    34561 agtcttttgc gtggtaacag cgcgctggta gatgagtgtg tcgcgtttta tgaagaacac
    34621 ttcccggatc gctattttct cgagctgatc cgcaccggca ggccggatga agaaagctat
    34681 ctgcacgcgg cggtggaact ggcggaagcg cgcggtttgc ccgtcgtggc gactaacgat
    34741 gtgcgcttta tcgacagcag cgactttgac gcacacgaaa tccgcgtcgc gatccacgac
    34801 ggctttaccc tcgacgatcc taaacgcccg cgtaactatt cgccgcagca atatatgcgt
    34861 agcgaagagg agatgtgtga gctgtttgcc gacatcccag aagcccttgc caacaccgtt
    34921 gagatcgcca aacgctgtaa cgtaactgtg cgtcttggtg aatacttcct gccgcagttc
    34981 ccgaccgggg acatgagcac cgaagattat ctggtcaagc gtgcaaaaga aggcctggaa
    35041 gagcgtctgg catttttatt ccctgacgag gaagaacgtc ttaaacgccg cccggaatat
    35101 gacgaacgtc tggagactga acttcaggtt atcaaccaga tgggcttccc gggctacttc
    35161 ctcatcgtta tggaatttat ccagtggtcg aaagataacg gcgtaccggt agggccaggc
    35221 cgtggctccg gtgcgggttc actggtggcc tacgcgctga aaatcaccga cctcgatccg
    35281 ctggaatttg acctgctgtt cgaacgtttc cttaacccgg aacgtgtctc catgcctgac
    35341 ttcgacgttg acttctgtat ggagaaacgc gatcaggtta tcgaacacgt ggcggacatg
    35401 tacggtcgtg atgcggtatc gcagattatc accttcggta cgatggcggc gaaagcggtg
    35461 atccgcgacg taggccgcgt gctggggcat ccgtacggct ttgtcgaccg tatctcgaaa
    35521 ctgatcccgc ccgatccggg gatgacgctg gcgaaagcgt ttgaagccga gccgcagctg
    35581 ccggaaatct acgaagcgga tgaagaagtt aaggcgctga tcgacatggc gcgcaaactg
    35641 gaaggggtca cccgtaacgc cggtaagcac gccggtgggg tggttatcgc gccgaccaaa
    35701 attaccgatt ttgcgccgct ttattgcgat gaagagggca aacatccggt cacccagttt
    35761 gataaaagcg acgttgaata cgccggactg gtgaagttcg acttccttgg tttgcgtacg
    35821 ctcaccatca tcaactgggc gctggagatg atcaacaagc ggcgggcgaa gaatggcgag
    35881 ccaccgctgg atatcgccgc gatcccgctg gatgacaaga aaagcttcga catgctgcaa
    35941 cgctcggaaa ccacggcggt attccagctt gaatcgcgcg gcatgaagga cctgatcaag
    36001 cgtctgcaac ctgactgctt cgaagatatg atcgcactgg tggcactgtt ccgccctggt
    36061 ccgttgcagt cagggatggt ggataacttt atcgaccgta aacatggtcg tgaagagatc
    36121 tcctatccgg acgtgcagtg gcagcatgaa agcctgaaat cggtactgga gccaacctac
    36181 ggcattatct tgtatcagga acaggtcatg cagattgccc aggtgctttc tggttatacc
    36241 ctcggtggcg cggatatgct gcgccgtgcg atgggtaaga aaaagccgga agagatggct
    36301 aagcagcgtt ctgtatttgc tgaaggtgca gaaaagaacg gaatcaacgc cgaactggcg
    36361 atgaaaatct tcgacctggt ggagaaattc gcgggttacg gatttaacaa atcgcactct
    36421 gcggcctatg ctttggtgtc atatcaaacg ttatggctga aagcgcacta tccggcggag
    36481 tttatggcgg cggtaatgac cgccgatatg gacaacaccg agaaggtggt gggcctggtg
    36541 gatgagtgct ggcggatggg gctgaaaatc ctgccaccag atataaactc cggtctttac
    36601 catttccacg tcaacgacga tggcgaaatc gtgtatggta ttggcgccat caaaggggta
    36661 ggtgaaggtc cgattgaggc catcatcgaa gcccgtaata aaggcggcta cttccgcgaa
    36721 ctgtttgatc tctgcgcccg taccgacacc aaaaagttaa accggcgggt gctggaaaaa
    36781 ctgatcatgt ccggggcgtt tgaccgtctt gggccacacc gcgcggcgct gatgaactcg
    36841 ctgggtgatg cgttaaaagc ggcagatcaa cacgcgaaag cggaagctat cggtcaggcc
    36901 gatatgttcg gcgtgctggc cgaagagccg gaacaaattg aacaatccta cgccagctgc
    36961 caaccgtggc cggagcaggt ggtattagat ggggaacgtg aaacgttagg cctgtacctg
    37021 accggacacc ctatcaacca gtatttaaaa gagattgagc gttatgtcgg aggcgtaagg
    37081 ctgaaagaca tgcacccgac agaacgtggt aaagtcatca cggctgcggg gctcgttgtt
    37141 gccgcgcggg ttatggtcac caagcgcggc aatcgtatcg gtatctgcac gctggatgac
    37201 cgttccgggc ggctggaagt gatgttgttt actgacgccc tggataaata ccagcaattg
    37261 ctggaaaaag accgcatact tatcgtcagc ggacaggtca gctttgatga cttcagcggt
    37321 gggcttaaaa tgaccgctcg cgaagtgatg gatattgacg aagcccggga aaaatatgct
    37381 cgcgggcttg ctatctcgct gacggacagg caaattgatg accagctttt aaaccgactc
    37441 cgtcagtctc tggaacccca ccgctctggg acaattccag tacatctcta ctatcagagg
    37501 gcggatgcac gcgcgcggtt gcgttttggc gcgacgtggc gtgtctctcc gagcgatcgt
    37561 ttattaaacg atctccgtgg cctcattggt tcggagcagg tggaactgga gtttgactaa
    37621 tacaggaata ctatgagtct gaatttcctt gattttgaac agccgattgc agagctggaa
    37681 gcgaaaatcg attctctgac tgcggttagc cgtcaggatg agaaactgga tattaacatc
    37741 gatgaagaag tgcatcgtct gcgtgaaaaa agcgtagaac tgacgcgtaa aatcttcgcc
    37801 gatctcggtg catggcagat tgcgcaactg gcacgccatc cgcagcgtcc ttataccctg
    37861 gattacgttc gcctggcatt tgatgaattt gacgaactgg ctggcgaccg tgcgtatgca
    37921 gacgataaag ctatcgtcgg tggtatcgcc cgtctcgatg gtcgtccggt gatgatcatt
    37981 ggtcatcaaa aaggtcgtga aaccaaagaa aaaattcgcc gtaactttgg tatgccagcg
    38041 ccagaaggtt accgcaaagc actgcgtctg atgcaaatgg ctgaacgctt taagatgcct
    38101 atcatcacct ttatcgacac cccgggggct tatcctggcg tgggcgcaga agagcgtggt
    38161 cagtctgaag ccattgcacg caacctgcgt gaaatgtctc gccttggcgt accggtagtt
    38221 tgtacggtta tcggtgaagg tggttctggc ggtgcgctgg cgattggcgt gggcgataaa
    38281 gtgaatatgc tgcaatacag cacctattcc gttatctcgc cggaaggttg tgcgtccatt
    38341 ctgtggaaga gcgccgacaa agcgccgctg gcggctgaag cgatgggtat catcgctccg
    38401 cgtctgaaag agctgaaact gatcgactcc atcatcccgg aaccgctggg cggcgctcac
    38461 cgtaacccgg aagcgatggc ggcatcgttg aaagcgcaac tgctggcgga tctggccgat
    38521 ctcgatgtgt taagcactga agatttaaaa aatcgtcgtt atcagcgcct gatgagctac
    38581 ggttacgcgt aattcgcaaa agttctgaaa aagggtcact tcggtggccc ttttttatcg
    38641 ccacggtttg agcaggctat gattaaggaa ggattttcca ggaggaacac atgaacatca
    38701 ttgccattat gggaccgcat ggcgtctttt ataaagatga gcccatcaaa gaactggagt
    38761 cggcgctggt ggcgcaaggc tttcagatta tctggccaca aaacagcgtt gatttgctga
    38821 aatttatcga acataaccct cgaatttgcg gcgtgatttt tgactgggat gagtacagtc
    38881 tcgatttatg cagcgatatc aatcaactta atgaatatct cccgctttat gccttcatca
    38941 acacccactc gacgatggat gtcagcgtgc aggatatgcg gatggcgctc tggttttttg
    39001 aatatgcgct ggggcaggcg gaagatatcg ccattcgtat gcgtcagtac accaacgaat
    39061 atcttgataa cattacgccg ccgttcacga aagccttgtt tacctacgtc aaagagcgga
    39121 agtacacctt ttgtacgccg gggcatatgg gcggcaccgc atatcaaaaa agcccggttg
    39181 gctgtctgtt ttatgatttt ttcggcggga atacccttaa ggctgatgtc tctatttcgg
    39241 tcaccgagct tggttcgttg cttgaccaca ccgggccaca cctggaagcg gaagagtaca
    39301 tcgcgcggac ttttggcgcg gaacagagtt atatcgttac caacggaata tcgacgtcga
    39361 acaaaattgt gggtatgtac gccgcgccat ccggcagtac gctgttgatc gaccgcaact
    39421 gccataaatc actggcgcat ctgttgatga tgaacgatgt agtgccagtc tggctgaaac
    39481 cgacgcgtaa tgcgttgggg attctggggg ggatcccgcg ccgtgaattt actcgcgaca
    39541 gcatcgaaga gaaagtcgct gccactacgc aagcacaatg gccggttcat gcggtgatca
    39601 ccaactccac ctatgatggt ttgctctaca acaccgactg gatcaaacag acgctggatg
    39661 tcccgtcgat ccacttcgat tccgcctggg tgccgtatac tcattttcat ccaatctacc
    39721 agggtaaaag tggtatgagc ggcgagcgtg ttgcgggaaa agttttcttc gaaacgcaat
    39781 cgacccacaa aatgctggcg gcgttatcgc aggcttcact gatccacatt aaaggcgagt
    39841 atgacgaaga ggcgtttaac gaagccttta tgatgcatac caccacctcg cccagttatc
    39901 ccattgttgc ttcggttaag acggcggcgg cgatgctgcg tggtaatccg ggcaaacggc
    39961 tgattaaccg ttcggtggag cgggcgctgc attttcgcaa agaggtccag cggctgcggg
    40021 aagagtctga cggttggttt ttcgatatct ggcaaccgcc gcaggtggat gaagccgaat
    40081 gcaggcccgt tgcgcctggc gaacaatggc acggctttaa cgatgcggat gccgatcata
    40141 tgtttctcga tccggttaaa gtcactattt tgacaccggg gatggacgaa cagggcaata
    40201 tgagtgagga ggggatcccg gcggcgctgg tagcaaaatt cctcgacgaa cgtgggatcg
    40261 tagtagagaa aaccggccct tataacctgc tgtttctctt tagtattggc atcgataaaa
    40321 ccaaagcaat gggattattg cgtgggttga cggaatttaa gcgctcttac gatctcaacc
    40381 tgcggatcaa aaatatgctg cccgatctct atgctgaaga tcccgatttc taccgcaata
    40441 tgcgtattca ggatctggcg caagggatcc ataagctgat tcgtaaacac gatctttccg
    40501 gtttgatgtt gcgggcattc gatactttgc cggaaatgat catgacgcca catcaggcat
    40561 ggcagagaca gattaaaggt gaagtagaaa ccattgcgct ggaacaactg gtcggtagag
    40621 tatcggcaaa tatgatcctg ccttatccac cgggcgtacc gctgttgatg ccaggagaaa
    40681 tgctgaccaa agagagccgc acggtactcg attttctact gatgctttgt tccgtagggc
    40741 aacattaccc cggttttgaa acggatattc acggcgcgaa acaggacgaa gacggcgttt
    40801 accgcgtacg agtcctaaaa atggcgggat aacttgccag agcggcttcc gggcgagtaa
    40861 cgtgctgtta acaaataaag gagacgttat gctgggttta aaacaggttc accatattgc
    40921 gattattgcg acggattatg cggtgagcaa agctttctac tgcgatattc ttggtttcac
    40981 gctgcaaagc gaagtctatc gcgaagcgcg cgactcgtgg aaaggggatt tggcgcttaa
    41041 tgggcaatat gtgattgagc ttttctcttt tccgttcccg ccggagcgcc ccagccgacc
    41101 ggaagcttgc ggcctgcgtc atctggcttt tagcgttgat gacatcgatg cggcagtggc
    41161 gcaccttgaa agccataacg tgaagtgtga agccatccgt gtcgatccat acacgcaaaa
    41221 acgcttcacc ttctttaacg atccggacgg gctgccgttg gaactgtatg agcagtaagg
    41281 cttgtcatcg ccgcatttgc ccggtaacgt gccgggcatt gctactgtaa aatcgcacca
    41341 tcatgacact cacgctcaat agacaacttc tcacctcacg ccagattctg gtggccttta
    41401 gcggcgggct tgactccacc gttctgctgc atcagttggt gcagtggcgg acggaaaatc
    41461 cgggcgtcac tctgcgcgct attcatgtgc atcacggttt aagtgccaat gccgatgcct
    41521 gggttacgca ttgcgaaaac gtctgccaac agtggcaggt gccgctggtg gtcgaacgcg
    41581 tacaacttgc gcaagagggg ctggggattg aggcccaggc gcggcaggca cgttatcagg
    41641 catttgcccg taccctattg cccggcgaag tgctggtcac cgcgcaacat ctcgacgatc
    41701 aatgtgaaac ctttctgctg gcgctaaaac gcggtagcgg ccctgccggg ctttcggcta
    41761 tggcggaagt ttcggagttt gctggaacac ggcttatccg cccgttgctt gcccgcacgc
    41821 ggggggaact ggagcagtgg gcattggcgc atggtttacg ctggattgaa gacgaaagta
    41881 atcaggacga ctcatacgat cgtaactttc tgcgcctgcg cgtagtgccg ttattgcagc
    41941 agcgttggcc gcattttgcg gaagcaacgg cccgcagcgc cacactttgt gctgaacaag
    42001 agagtctgtt ggatgaactg ctggcggatg atttagcaca ctgccaaacg tcgcagggga
    42061 cgctgcagat tgcgccaatg ctggcgatga gtgatgcccg tcgcgcggcg attattcgcc
    42121 gctggctggc agggcagaat gcaccgatgc cttcccgcga cgcgctggtg cgcatctggc
    42181 aggaagtggc gctggcgcgg gaagatgctt ccccctgttt acgtttgggc gcctttgaaa
    42241 tccgccgcta tcagtcgcaa ctgtggtgga ttaaatccgt caccgggcaa agcgaaacca
    42301 ttgtgctgtg gcagacgtgg ctacaaccgc tggaattacc ggcggggctg ggaaccgtac
    42361 agcttactgc gggaggcgat attcgccctc cgcgtgcaga cgaagcggtc agcgtgcgtt
    42421 tcaaagcgcc aggattgctg catattgtcg ggcgcaacgg cggacgtaag ctgaagaaaa
    42481 tctggcaaga gttaggcgtg ccgccgtggg tacgtgacac cacgccactg ctgttttatg
    42541 gagaaacgct gattgcggcg gcaggggtat ttgtgacgca agaaggtgtg gctgaaggtg
    42601 agaatggcgt aagttttgtc tggcagaaaa cgcttagtta agtgaaagcc ggataagacg
    42661 catcaaacgt cgcatccggc gaaagtcaat caggattcgc ttaccaccac cgtaccgatt
    42721 tcagggtggc taaagctggt aattttatcc agacgcagct cgcgggtttc gccagcggcc
    42781 tcgacgacca gatactccac atttttgcgg gagactaaat cactggcttt tgcctgcaat
    42841 ttttcgccat ctttcagctc aagtgtcagc attaaatgat gctggcaggc gagctcaaga
    42901 ttatcgtaat catcacaatt aattggttga tacgtatcat tcattgacat aatcgctcac
    42961 cagtaagttt gccgcagcgt atgctgcttt ttccctgaca gcctcagaaa gggcgtcgtc
    43021 ggcagccatt tcattcagca ctttcaaaac gcagcccagc gcgtccggaa cgtatcctaa
    43081 gtctccgctg gcgatttccg cgtaccgctt gcgtattaac tcacaatatt tttccacatg
    43141 ccctcctgtc agcactctga cttaaccgtg gatgcaagtc taagcctacg aagataaact
    43201 ctgtttcgca aggtgactat accacactca tttctgcaat atcagcgccg caactgcacg
    43261 tattccgtta caatggcctc ctgattcgaa aggagttttc ttatggcgct taaagcgaca
    43321 atttataaag cgacggttaa tgtggccgat ctcgaccgca accagtttct cgatgcctct
    43381 ctgacgctgg cgcgccaccc ttcagaaacc caggagcgta tgatgctgcg cttgctggcg
    43441 tggctgaaat atgctgatga acgtctgcaa tttacccgtg gtttgtgtgc cgatgatgag
    43501 ccggaagcgt ggctgcgtaa cgatcacctg ggcattgatt tgtggattga gctggggctg
    43561 ccggatgagc gacgaattaa gaaagcctgc acccaggccg cagaagtggc gctgtttacc
    43621 tataatagtc gggcggcgca aatctggtgg cagcaaaatc agagcaaatg tgtgcagttt
    43681 gccaatcttt ccgtctggta tctggacgat gaacaactgg cgaaagtaag cgcctttgcc
    43741 gatcgtacca tgacgctgca ggcaacgatt caggatggtg ttatctggtt atcggatgat
    43801 aagaataatc tggaagttaa cttaaccgcc tggcaacaac cttcatgatt gtgatttccc
    43861 gacatgttgc tattcccgat ggtgagcttg agatcaccgc cattcgtgcg cagggcgctg
    43921 gcgggcagca tgttaataag acctcaacgg ctattcatct gcgttttgac attcgggcgt
    43981 ccagcctgcc agagtattac aaagagcgtc tgctcgccgc cagccatcat ttgatcagca
    44041 gtgatggcgt gattgtcatt aaggcacagg aataccgcag tcaggaactg aaccgcgaag
    44101 ccgctctggc ccggctggtg gcagtgatta aagatttaac aaaagaacaa aaagcccgac
    44161 gacccacgcg gcccacccgt gcatcgaaag agcgcaggct ggcatcgaaa gcacaaaaat
    44221 caagcgtgaa ggcgatgcgc ggcaaagtac gcagcggtcg ggaataaaaa gaaggaatgg
    44281 atggtgaaaa aagcgatagt gacagcgatg gctgtaatca gcctctttac tctgatggga
    44341 tgtaataatc gggccgaagt cgatacgctt tctccggcgc aggctgccga actgaaaccg
    44401 atgccgcaaa gttggcgcgg cgtgctaccg tgtgccgatt gcgaaggaat cgaaatctct
    44461 ctgttcctcg aaaaagacgg aacatgggtg atgaatgagc gttatctcgg tgctcgtgaa
    44521 gaaccttcct ccttcgcttc ctacggtaca tgggcgcgaa ccgctgacaa gctggtatta
    44581 accgacagca aaggtgaaaa gtcatattat cgggcgaaag gcgatgcgct ggagatgctc
    44641 gatcgtgaag gcaatccaat tgaatcgcag ttcaactata cgctggaacc ggcacaatcc
    44701 agtttaccta tgacgccgat gaccctgcga ggcatgtatt tttatatggc tgatgcggcg
    44761 accttcactg attgcgcgac cggaaaacgt ttcatggtag cgaataacgc agagctggag
    44821 cgtagctacc tggctgcgcg cggtcacagt gaaaaaccgg tgttactgtc agtagaaggt
    44881 cactttacgc ttgaggctaa tccggatact ggtgcgccaa ccaaagtatt agcgcccgat
    44941 acggcgggta aattttaccc gaataaggat tgcagtagtt tggggcagta aacctgctta
    45001 aggcagaaac aaacgcaaaa ttgcctgatg cgctgcgctt atcaggccta cagcatataa
    45061 tgcaacattt tgaattagca cgattttgta ggccggataa ggcgtttacg ccgcatccgg
    45121 cattttgtgc acgaggctgg ctttatcact gagtcagccc aacaaagcga cccgccttga
    45181 tataaatccc aggcttcagg tgcccaacat actgtaactg cgtttctggc ttaaacgcag
    45241 agacatcacc aatacgcata tgcatcagat cggcagggct aatccagccc gattgcgcca
    45301 gtgtaagtgg atgcccggat ttggcaaatg catcagtgac aaattccgaa caaaaccacg
    45361 actttttgtc tccttcgcac acactgctta actgcgcttt cgccaggtcg ctgacgcact
    45421 gttggcgaaa atcctcggag aacggattca gtgagcacat ctggcgagtc accataaagg
    45481 gaataaattc gacaatgccg cgatagttat aaccgctatc tttgatttta ttggcaaacg
    45541 cggtgatatc tgtggcttgt tgcggggtaa gatccgggac tcgtaagacg aaaagcttat
    45601 cactatgctt catcgctttt ttaagggaaa caatctggac gccagcgcct gtcgcttctg
    45661 caacgttatt atcacccaga aagattgcaa cgtgactcac agaggaagtg ctgaagacgc
    45721 ggattccaaa tgaggttacc ccaaggcttg aggagaacag caaatcgccg ggtttgagat
    45781 caggtgccgt tatttctttt attgattgtt cggtgaaaga gctttgatgc tggaatttga
    45841 cagcccatgt ttttgcctcg gcatctactg ctgtggctga tgggtctggc tggctgatat
    45901 caacggtaca agccgaaagt aaaagaaagc tggggagaag caggcggcag tacgcctttg
    45961 gtttatccat tttatacaat ccatgtaaaa aagggccctg aaattcagga ccctttctgg
    46021 catcagcctt taatctgttt caccagatat tcgacgatgt caccagtctt aattaactgt
    46081 ttctcgccgt tgcgacgata tttatattcg atatcgtcgt tgtcgaggtt acggtcgccc
    46141 agcacaatag tgtgcggaat accgatcagt tccatatcag caaacatcac gcccggacgc
    46201 tctttgcggt catccagcag cacttcgata ccttgtgcac gcagttcgct gtacagtttc
    46261 tcagcaagct cttgtacgcg gaaggatttg tgcatgttca tcggcagaat cgccacctgg
    46321 aacggcgcga tagcgtcagg ccatacgatg ccgcgttcgt cgtagttctg ctcaatcgcc
    46381 gcagctacca cacgcgttac cccgataccg tagcaaccca tcgtcaggat ttggttacgg
    46441 ccatcttcac cctgtacgga ggctttcagt gcttcggagt acttggtacc cagctggaag
    46501 atgtgaccaa cttcgatacc acgtttgatc agcagcgtac cctggccatc cgggcttgga
    46561 tcgccagcca ccacgttacg gatatctgca acttccgggg tagcgacatc gcgatcccag
    46621 ttgataccga agtagtgttt accatcgatg ttagcaccag cagcgaaatc actcatcgcc
    46681 gcaacggtac ggtcaatcac caccggaatc ggcatgttta ccggacccag tgaacccgga
    46741 ccggctttaa ccacagcacg aatttcttct tcggtcgcga aagtcagcgg gcttgcaacc
    46801 tgcggcagtt tttctgcttt aacttcgttc agctcgtgat caccgcgcac cagcagcgca
    46861 accagcggga agctactgcc ttcaaccgct ttaaccagca gagtcttaac cgttttctca
    46921 atcggcagat tgaactgttc aaccagttcc gcgatggttt tcgcgttcgg cgtatcaacc
    46981 agcgtcattt cctgggtagc agcagcgcgc ggttctttcg gcgcgatagc ttctgccagt
    47041 tcaatgttcg ctgcatagtc agaggtgtcg gagaagacca catcgtcttc accgctctgc
    47101 gccagcacct ggaattcgtg agaggcgctg ccgccgatag aaccggtgtc ggcctgtacg
    47161 gcgcggaaat ccagccccat gcggctgaag attttgctgt aggccgcata cattgcatcg
    47221 taggtttcct gcagggattc ctgagaagta tggaaagagt aagcatcttt catcaggaat
    47281 tcgcgggaac gcatgacgcc gaaacgcgga cgcacttcgt cgcggaactt ggtctggatc
    47341 tgatagaagt tcagcggcag ctgtttgtaa gagctaagct cgttacgaat caggtcagtg
    47401 ataacttctt catgagttgg gccgagtacg aacggacgct cgccacggtc aacaaaacgc
    47461 agcagttccg gaccgtactg ttcccaacga ccactctctt gccacaaatc ggctggctga
    47521 accaccggca tcgacacctc gatcgcaccg gcgttgttca tctcttcacg cacgatgttt
    47581 tcgacttttt tcagaacgcg cacgccggtc ggcagccagg tatataaccc ggaggccagc
    47641 ttgcggatca tcccggcgcg cagcatcagc tgatggctga tcacctcggc gtcggcaggt
    47701 gtctccttga gagtggagag caggtattgg ctagtacgca tgttgttacg gttccagttg
    47761 gaaggtagaa caggctcaag gcgagcctgg gacaaaaaaa gtgatttagt ttaccagtgc
    47821 aaaagaaatg tcaaaagaga agggcgtgaa tttaacgcgg ttccagcgca aagacttcaa
    47881 aacctgcgtc ggtgacgcgc cagcgaacgt taaaatcatg tagccagacg gcataggttt
    47941 tgcccgtttc ctcaccttta cgataggccg ggcgcgggtc ctgcgccagt acttcgcgga
    48001 taaacagcgt taactgcgga taacgcttct ccagcgtcaa aagctgcttt tcgacctctg
    48061 cggtaaaact caccgccatc tctgcagctg gcgcactttg cgcatagctg gcactggcat
    48121 cgggaagcga ttcggcaaag gggagatacg gtttgatatc cactaccggc gtaccatcga
    48181 ccagatccag actgccgagc ttcagaatca cgctgccttt atggcaaaca acctctttca
    48241 gctctaccag cgacatgcca attgggttag ggcggaaagt agagcgtgtt gcgaaaaccc
    48301 ccattctggc gttaccgccg aggcgcggtg gacgcacagt cggacgccag ccgccttcca
    48361 tcgtttgatg aaagacgaaa aggatccata aatggctgaa tgcttccagg ccgcgaacgg
    48421 cgtcggcctg gttgtaggaa gctatgagat gcagttcgcc gttggcgctt tttaccagac
    48481 ctggctggcg cggaacggcg aatttttctt tatagggcga gcgaataacg cctatttgct
    48541 cgaactggaa actgctcatt tcgccgtaat gttaagcgca gagccgatac atacagcctg
    48601 acgatagcag cccggcgtac cgctggtgac ttcgcagcta tgcagtaata cagcattggc
    48661 tttcatttta gaggcattga tttgcatctg cttacgtgcg gttggaatgc tcggcggaga
    48721 gtcctgatta gaggcctggc aagagtcgcc actgacttca ccgagatcgc ggaacggttt
    48781 gccgactaat tcttctgcat tggtataaat tcggaccggc gtggcgcgcg gcgctttcgg
    48841 ttttgcaggc tccgctttcg gctggggtgc agtgctttga acgggttcga caggggatct
    48901 gcttaacatg gaacagccgc ttagcatgag tgctactaaa cagatcggta aagcacgcat
    48961 agtatttcct caatgtatga tcaaaacgtc aatattgaat caggagcttg taaaaatgac
    49021 aagacgggca agcgcccgtc ctgaatgata ttacaaattg tggaaacagc ctaaaaatta
    49081 ccagccttta acagcgccgc cgttaaacac tttgtttgct gcttcgtaaa cttcatcaga
    49141 ctgataagcc tggacgaatt tcttcacgtt ctcggcgtct ttgttatctt cacgcgttac
    49201 gatcaggttt acgtatgggg agtctttatc ttcgacaaag ataccgtctt tcgccggagt
    49261 caggccaatc tggctggcat aggtggtatt gataactgcc agagcgattt gcgcgtcgtc
    49321 cagagaacgc ggcagttgcg gtgcttccag ttcaacaatt ttcagatttt ttgggttctc
    49381 aacaacatca agaacggtcg gcagcaggcc aacgccatct ttcagtttga tcaagcccac
    49441 tttttgcagc agcagcagtg aacgaccaag attagttgga tcgtttggca cggcaacctg
    49501 cgaaccatcc tgcagttcat ccagtgattt gattttcttg gagtaaccag caatcggata
    49561 aacaaaagtg ttgcctactg cgaccagttt gtagccacga tctttcagtt gctgatcaag
    49621 gtacggttta tgctggaagg cgttggcgtc gatatcgcct ttgctcaatg cttcgtttgg
    49681 cagaacatag tcgttgaagg ttaccagctc aacgtccagg ccatatttgt ctttcgcaac
    49741 tttctgcgcg acttctgcta cctgctgttc ggcaccaaca atcacgccga ctttaatgtg
    49801 gtttggatct ttttcatcct gaccgcagcc taccagtgcc agtgatccaa tcagggctcc
    49861 cactgccgca aaggttttga atttgaacgc cataccttat tccttcttca attatttatg
    49921 ttgtgttgaa cgttacttgc gagtgacagc ccggacgatg cggtcgcctg caaactgaat
    49981 taaataaacc agaatgacca gcaataccag taccgtattc atcaccgtcg cgttgtagcc
    50041 gatgtagcca tactgatagc caatctgacc taaaccaccg gcaccgactg caccacccat
    50101 cgcggaataa ccgaccaggg taatcagggt gatagttgcc gcattcacca gacccggcaa
    50161 cgcttccggt aacagcactt tacagacgat ctgcattggc gtggcaccca ttgcgcggga
    50221 agcttcaatt aacccggttg ggatctccag cagagcgttc tcgaccatac gggcaataaa
    50281 cggtgctgca ccaacggtta acggaacaat tgctgcctgc aaaccaatcg atgtaccgac
    50341 aataacgcgg gtaaacggaa tcatccatac cagcaagata atgaacggga tggaacggaa
    50401 aatgttcaca atcgcagaaa cggtacgata cagcttcgcg ttagcaataa tttgccccgg
    50461 acgcgtgaca taaagcagaa cgccaaccgg cagaccaatc acaaacccaa aaaatccgga
    50521 tacgaaggtc attgccagcg tttcccatac gccacgaacc agcagccaca tcatcggctc
    50581 agacataacc cagtacctct acttttacat ggtgttcctg cagccaggca atggcggctt
    50641 gcgtatcttg ttgtgtgccg tgcatttcag tcagcatgat gccgaattta acgccaccgg
    50701 cgtagtccat ctgcgcgcta ataatgttgt tgttgacgtt gaaacgacgc gcggtttcag
    50761 aaagcagtgg ggcatcgacc gattgaccgg taaactccag acgcagcatc ggcacgcagt
    50821 cagtaaatgg ctccgtttgc agacgttcct ggtaatcttc cgggatatcc agatgcaggg
    50881 tcgactgaat aaacttctgc gccagcggcg ttttcggatg cgagaacact tcacttaccg
    50941 tgtcctgctc gatcagttcg ccattgctga tgaccgccac gcaatcacaa atacgcttca
    51001 caacgtccat ttcgtgggtg atcaacagaa tcgtcaaacc cagacggcgg ttaatgtctt
    51061 tcagcagttc gagaatagaa cgtgtcgttg ccgggtccag cgcgctggtg gcttcatcac
    51121 acagcaatac tttgggattg ctggctaatg cacgggcaat cgccacacgt tgtttctgcc
    51181 caccggaaag attcgacggg tagctatcat gcttatcgcc aagaccaacc aatgacagca
    51241 attccgtcac gcgacgtttg atctcgtctt tcggtgtgtt gtccagctcc agcggcagag
    51301 ccacgttgcc aaaaacagta cgcgaagaga gcaggttaaa atgctggaaa atcataccaa
    51361 tctggcggcg agctttggtc aactcggatt ctgacagcgt ggtcaattcc tggccatcga
    51421 ccagcacgct accctcggtt gggcgctcca gcaagtttac acaacgtata agcgtactct
    51481 tacccgcgcc tgaggcaccg ataacgccat aaatttgtcc agctggcaca tgcaggctga
    51541 cgttgttcaa cgcctggatg gtgcgggtgc cctggtggaa cactttggtg atattcgaaa
    51601 gttttatcat tgattattta ttatcgtcat taagttagtc gtagcatctc gaatgcctga
    51661 aacgggcaac gccgtcaatg aaatggatgt taaggcatcc agacgtctaa atcaatcagg
    51721 tttatgcgaa gagcactttc ttgcaggtcg aaacatgcga tactagcgtc ccatgcctta
    51781 ttaaggagct ataaaaggtg gcgaagagcg tacccgcaat ttttcttgac cgtgatggca
    51841 ccattaatgt cgatcacggc tatgtccatg agatcgacaa ctttgaattt atcgacggtg
    51901 ttattgacgc catgcgcgag ctaaaaaaaa tgggctttgc gctggtggta gtaaccaacc
    51961 agtctggcat tgctcgcggt aaatttaccg aagcacagtt tgaaacgctg accgagtgga
    52021 tggactggtc gctggcggac cgagatgtcg atctggatgg tatctattat tgcccgcatc
    52081 atccgcaggg gagtgttgaa gagtttcgcc aggtctgcga ttgccgcaaa ccacatccgg
    52141 ggatgctttt gtcagcacgc gattatttgc atattgatat ggccgcttct tatatggtgg
    52201 gcgataaatt agaagatatg caggcagctg ttgcggcgaa cgtgggaaca aaagtgctgg
    52261 tgcgtacggg taaacctatt acacctgaag cagaaaacgc ggcagattgg gtgttaaata
    52321 gcctggcaga cctgccgcaa gcgataaaaa agcagcaaaa accggcgcaa tgattaaaag
    52381 atgaacggtt gaaataaaaa tgcatttttc cgcttgtctt cctgagccga ctccctataa
    52441 tgcgcctcca tcgacacggc ggatgtgaat cacttcacac aaacagccgg ttcggttgaa
    52501 gagaaaaaat cctgaaattc agggttgact ctgaaatagg aaagtgtgat atacgccacc
    52561 tcgcgacagt gagct
//
LOCUS       DAJTSI010000010        51024 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000010 DAJTSI010000000
VERSION     DAJTSI010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 51024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51024
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.010"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..950)
                     /gene="nhaA"
                     /locus_tag="OO327_000523"
     CDS             complement(<1..950)
                     /gene="nhaA"
                     /locus_tag="OO327_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR6017189.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLA"
     gene            1536..1688
                     /gene="mokC"
                     /locus_tag="OO327_000524"
     CDS             1536..1688
                     /gene="mokC"
                     /locus_tag="OO327_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR6017190.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(1792..2922)
                     /gene="dnaJ"
                     /locus_tag="OO327_000525"
     CDS             complement(1792..2922)
                     /gene="dnaJ"
                     /locus_tag="OO327_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR6017191.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(3011..4927)
                     /gene="dnaK"
                     /locus_tag="OO327_000526"
     CDS             complement(3011..4927)
                     /gene="dnaK"
                     /locus_tag="OO327_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR6017192.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            5304..5708
                     /locus_tag="OO327_000527"
     CDS             5304..5708
                     /locus_tag="OO327_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR6017193.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            5734..6447
                     /gene="msyB"
                     /locus_tag="OO327_000528"
     CDS             5734..6447
                     /gene="msyB"
                     /locus_tag="OO327_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR6017194.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            6596..7162
                     /gene="satP"
                     /locus_tag="OO327_000529"
     CDS             6596..7162
                     /gene="satP"
                     /locus_tag="OO327_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR6017195.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(7197..7784)
                     /gene="mog"
                     /locus_tag="OO327_000530"
     CDS             complement(7197..7784)
                     /gene="mog"
                     /locus_tag="OO327_000530"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR6017196.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(7899..8852)
                     /gene="tal"
                     /locus_tag="OO327_000531"
     CDS             complement(7899..8852)
                     /gene="tal"
                     /locus_tag="OO327_000531"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6017197.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            9131..10561
                     /locus_tag="OO327_000532"
                     /pseudo
     CDS             9131..10561
                     /locus_tag="OO327_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            10631..11407
                     /gene="yaaA"
                     /locus_tag="OO327_000533"
     CDS             10631..11407
                     /gene="yaaA"
                     /locus_tag="OO327_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR6017198.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(11560..11856)
                     /locus_tag="OO327_000534"
     CDS             complement(11560..11856)
                     /locus_tag="OO327_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6017199.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(12070..13356)
                     /gene="thrC"
                     /locus_tag="OO327_000535"
     CDS             complement(12070..13356)
                     /gene="thrC"
                     /locus_tag="OO327_000535"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR6017200.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(13357..14289)
                     /gene="thrB"
                     /locus_tag="OO327_000536"
     CDS             complement(13357..14289)
                     /gene="thrB"
                     /locus_tag="OO327_000536"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR6017201.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNKLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(14291..16753)
                     /gene="thrA"
                     /locus_tag="OO327_000537"
     CDS             complement(14291..16753)
                     /gene="thrA"
                     /locus_tag="OO327_000537"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR6017202.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(16779..16898)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(16834..16899)
                     /gene="thrL"
                     /locus_tag="OO327_000538"
     CDS             complement(16834..16899)
                     /gene="thrL"
                     /locus_tag="OO327_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR6017203.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(17113..17799)
                     /locus_tag="OO327_000539"
     CDS             complement(17113..17799)
                     /locus_tag="OO327_000539"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6017204.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(18199..18339)
                     /locus_tag="OO327_000540"
     CDS             complement(18199..18339)
                     /locus_tag="OO327_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6017205.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            18435..19151
                     /gene="arcA"
                     /locus_tag="OO327_000541"
     CDS             18435..19151
                     /gene="arcA"
                     /locus_tag="OO327_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR6017206.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(19210..20562)
                     /gene="creD"
                     /locus_tag="OO327_000542"
     CDS             complement(19210..20562)
                     /gene="creD"
                     /locus_tag="OO327_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR6017207.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(20620..22044)
                     /gene="creC"
                     /locus_tag="OO327_000543"
     CDS             complement(20620..22044)
                     /gene="creC"
                     /locus_tag="OO327_000543"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR6017208.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(22044..22733)
                     /gene="creB"
                     /locus_tag="OO327_000544"
     CDS             complement(22044..22733)
                     /gene="creB"
                     /locus_tag="OO327_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR6017209.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALYPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(22746..23219)
                     /gene="creA"
                     /locus_tag="OO327_000545"
     CDS             complement(22746..23219)
                     /gene="creA"
                     /locus_tag="OO327_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR6017210.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            23430..24299
                     /gene="robA"
                     /locus_tag="OO327_000546"
     CDS             23430..24299
                     /gene="robA"
                     /locus_tag="OO327_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR6017211.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(24296..24943)
                     /gene="gpmB"
                     /locus_tag="OO327_000547"
     CDS             complement(24296..24943)
                     /gene="gpmB"
                     /locus_tag="OO327_000547"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR6017212.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            24995..25515
                     /gene="yjjX"
                     /locus_tag="OO327_000548"
                     /pseudo
     CDS             24995..25515
                     /gene="yjjX"
                     /locus_tag="OO327_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(25600..25926)
                     /gene="trpR"
                     /locus_tag="OO327_000549"
     CDS             complement(25600..25926)
                     /gene="trpR"
                     /locus_tag="OO327_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR6017213.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(26016..27953)
                     /gene="sltY"
                     /locus_tag="OO327_000550"
     CDS             complement(26016..27953)
                     /gene="sltY"
                     /locus_tag="OO327_000550"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR6017214.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            28164..29831
                     /gene="ettA"
                     /locus_tag="OO327_000551"
     CDS             28164..29831
                     /gene="ettA"
                     /locus_tag="OO327_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR6017215.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(29938..30105)
                     /locus_tag="OO327_000552"
     CDS             complement(29938..30105)
                     /locus_tag="OO327_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6017216.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(30139..31371)
                     /gene="nadR"
                     /locus_tag="OO327_000553"
     CDS             complement(30139..31371)
                     /gene="nadR"
                     /locus_tag="OO327_000553"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR6017217.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(31392..32774)
                     /gene="radA"
                     /locus_tag="OO327_000554"
     CDS             complement(31392..32774)
                     /gene="radA"
                     /locus_tag="OO327_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR6017218.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(32823..33791)
                     /gene="serB"
                     /locus_tag="OO327_000555"
     CDS             complement(32823..33791)
                     /gene="serB"
                     /locus_tag="OO327_000555"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR6017219.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            33897..34541
                     /locus_tag="OO327_000556"
     CDS             33897..34541
                     /locus_tag="OO327_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR6017220.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            34569..35584
                     /gene="lplA"
                     /locus_tag="OO327_000557"
                     /pseudo
     CDS             34569..35584
                     /gene="lplA"
                     /locus_tag="OO327_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            35616..35879
                     /locus_tag="OO327_000558"
     CDS             35616..35879
                     /locus_tag="OO327_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6017221.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(36040..36759)
                     /gene="deoD"
                     /locus_tag="OO327_000559"
     CDS             complement(36040..36759)
                     /gene="deoD"
                     /locus_tag="OO327_000559"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR6017222.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(36816..38039)
                     /gene="deoB"
                     /locus_tag="OO327_000560"
     CDS             complement(36816..38039)
                     /gene="deoB"
                     /locus_tag="OO327_000560"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR6017223.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(38091..39413)
                     /gene="deoA"
                     /locus_tag="OO327_000561"
     CDS             complement(38091..39413)
                     /gene="deoA"
                     /locus_tag="OO327_000561"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR6017224.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(39540..40319)
                     /gene="deoC"
                     /locus_tag="OO327_000562"
     CDS             complement(39540..40319)
                     /gene="deoC"
                     /locus_tag="OO327_000562"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR6017225.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            40577..42118
                     /locus_tag="OO327_000563"
     CDS             40577..42118
                     /locus_tag="OO327_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR6017226.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            42090..42953
                     /locus_tag="OO327_000564"
     CDS             42090..42953
                     /locus_tag="OO327_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR6017227.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(43167..43949)
                     /locus_tag="OO327_000565"
     CDS             complement(43167..43949)
                     /locus_tag="OO327_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6017228.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(43946..45019)
                     /locus_tag="OO327_000566"
     CDS             complement(43946..45019)
                     /locus_tag="OO327_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR6017229.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(45141..45302)
                     /locus_tag="OO327_000567"
     CDS             complement(45141..45302)
                     /locus_tag="OO327_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR6017230.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(45419..46024)
                     /gene="osmY"
                     /locus_tag="OO327_000568"
     CDS             complement(45419..46024)
                     /gene="osmY"
                     /locus_tag="OO327_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR6017231.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(46417..48006)
                     /gene="prfC"
                     /locus_tag="OO327_000569"
     CDS             complement(46417..48006)
                     /gene="prfC"
                     /locus_tag="OO327_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR6017232.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(48097..48774)
                     /gene="yjjG"
                     /locus_tag="OO327_000570"
     CDS             complement(48097..48774)
                     /gene="yjjG"
                     /locus_tag="OO327_000570"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR6017233.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(48789..49235)
                     /gene="rimI"
                     /locus_tag="OO327_000571"
     CDS             complement(48789..49235)
                     /gene="rimI"
                     /locus_tag="OO327_000571"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6017234.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(49204..49617)
                     /gene="holD"
                     /locus_tag="OO327_000572"
     CDS             complement(49204..49617)
                     /gene="holD"
                     /locus_tag="OO327_000572"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR6017235.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            49720..50751
                     /gene="rsmC"
                     /locus_tag="OO327_000573"
     CDS             49720..50751
                     /gene="rsmC"
                     /locus_tag="OO327_000573"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR6017236.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT        12073 a        13851 c        12835 g        12265 t
ORIGIN      
        1 gccagtttca aacgcagcgc caaccagcag aacagactaa tccccagcgg tttgccaatc
       61 agcaagccag cgatgatccc caatggcaga atggaggtca agccatccag cgtgacgcct
      121 tgcagtgaaa cgccagcatt agcaaatgca aacagcggca aaatcagata cgccacccac
      181 gggtgcaaca catgctccag tcgcttcgct ggagaacgcc catgcttctc tttcaaagga
      241 ataaagaagc cgacaattac ccccgccaga gttgcgtgaa cccccgattt caacaccgca
      301 gtccacaaca ccacgccaac aagaatatag acgcccgtgc ggcgtacacc acacagattc
      361 aataccgcga gtaccgcaat tgctacagcc gcgacgccaa gggaggccat cgataagtca
      421 ttagtgtaga acaatgcgat gataatgatg gccccaagat cgtcgataat agccagagcc
      481 atcaaaaaga tcttcagcac taacggaaca cgacttccca acagcgccag tacaccaagt
      541 gcaaaagcaa tgtcagtagc cgccgggatc gcccaccctt cgcgggtaat cggatcggca
      601 tagttaaaag ccagatagag taatgccggc acaatcatcc caccaatagc ggcgataact
      661 ggaaatgcgg cctggcgtaa gctggctagc gatccttgca tcagttcacg tttaacttcc
      721 agaccgacta acaggaaaaa taccgccatc agcgcatcat ttatccataa cagcatgttt
      781 ttgttgattt cgagtgaacc aacccggagc tgaaccggcg tctccagaaa gtcgtgatac
      841 catccactgg ttgcgccgct gttggccata atcatcgcca ggatagcggc aatgataaga
      901 ataatgcctc ccgaggcatc actgctaaag aatcgatgca gatgtttcac tttttatttc
      961 tctttcaggt gaatagatcg tttttactat tttaccccgc acgaatcatc gttaaaatca
     1021 gattatagtg taggaataat tcgtttttta cgagccattg aagaatttgc gccatcagcg
     1081 gtgagatgcg tttttgacgg ctgtaagcgc tcacgagtga ccctaagccc agataatcat
     1141 agcttaaggt gaggattcct gagggattaa ttaggaaaag taataattaa tttgttgtca
     1201 gtcggcgatg cttgttaggc caatatttca aaaataatcc agcttgtcgt caataattgc
     1261 gatgaagaga taaattaaag ttaaataatt aaatcattaa aaaattacgc cacaaaaatg
     1321 cgaggcgtct tcaggattag aatcgataaa acaaaatggg ttaaagagag cgatataata
     1381 gcggcgggtg cttgaggctg tctgtctcag gcattagctg aacggcagat agagaaaagc
     1441 cccgagtgat attttaccat caacccgagg cctcctatat gctgaacaca tgtagagtgc
     1501 ctcttactga ccgtaaggtc aaggagaaga gagcaatgaa gcagcataag gcgatgattg
     1561 tcgccctgat cgtcatctgt atcaccgccg tagtggcggc gctggtaacg agaaaagacc
     1621 tctgtgaggt tcacatccga actggccaga cggaggttgc tgttttcacg gcttacgaat
     1681 ccgagtaaga gcaacggcgg ggagtgatcc ccgccacttt acaggtgctc gcatatcttc
     1741 aacgcaccct atttttaccc aggcctgccc acgggcaggc ttttggggag gttagcgggt
     1801 caggtcgtca aaaaacttct tcacaccatc aaagaagctc tttgagcgcg ggctgttgtg
     1861 ctcgccggtt gggccaccga agctttcttg cagctcttgc agcagctgtt tctgcttttc
     1921 gttcaggcct accggtgttt cgacgacaac gcggcacagc aaatcaccct gtgcgccacc
     1981 gcggacagac ttgacgcctt taccgcgcat acggaatagc ttaccggtct gggtttcgcc
     2041 aggcactttc agtttgacgc gaccatcaag ggtcggtact tcgatttcgc cacccagcgc
     2101 cgccatagcg aagttgatcg ggacttcgca atacaggttg ttgccttcac gctcgaaaat
     2161 cgggtgctgt ttaacctgaa cctgaacgta cagatcgcct gccggtgcgc catgctcgcc
     2221 cgcttcacct tcgcccgcaa gacggatgcg gtctccagtg tccacccctg ccgggatttt
     2281 aacggacagc gttttgctgc gctcaacacg accatgacca tgacatttgt tgcacggatc
     2341 tttgatcagc gtaccgcggc cctgacagtg tggacaggtc tgctgtacag cgaagaatcc
     2401 ctggcgcatc tgcacctgac cagaaccatg acaggtcgga caggtctgcg gctgtgtacc
     2461 tggttttgca ccgctaccgt ggcaaacgtc acactcttcc agagtcggaa tgcggatctc
     2521 tttggtcacg ccacgtacag cttcttcgag ggtgagctcc atgttatagc gtaaatcagc
     2581 accgcgcgcc gcacgttgac gaccacgtcc gccgccaaaa atatcgccga aaacgtcacc
     2641 aaaaatatcg ctgaagtctg cgccgccgcc aaaaccaccg ccgcccatgc ctccttgctc
     2701 aaacgcagca tgaccatact gatcgtatgc cgcacgtttt tgcgagtcgg tcagaacttc
     2761 ataagcttcc ttgatctctt taaatttcgc ctcggcctct ttgtcaccct ggttacggtc
     2821 cgggtggtat ttcatggcca ggcgtttgta ggcctttctg atttcacgct cttccgctgt
     2881 tttggaaacg cctaaaatct cgtaataatc ttgcttagcc atctttttta aattgcccct
     2941 agatgaatgc acgggcggag aggaaattcc ccttcgcccg tgtcagtata attacccgtt
     3001 tatagggcga ttattttttg tctttgactt cttcaaattc agcgtcgaca acatcgtcat
     3061 ctttcgcgtt gtttgcagaa gcatcagcac cggcagtctg ctgctgggca tgttgctgct
     3121 gggcgatttc catcagtttc tgggaaacct gtgccagttc ctgcattttc gcttcgatag
     3181 cggctttgtc ttcacctttc agagcagttt ccagtgcagt cagcgcagac tcgatagcag
     3241 ttttgtcgtc agccggcagt ttgtcgcctg cttcttcaac ctgcttacgg gtgctgtgca
     3301 gcagatggtc gccctggttg cgagtctgta ccagctcttc aaacttacgg tcagcttcgg
     3361 cgttagcttc tgcgtcgcgt accattttct ggatttcatc ttcgttcaga ccagaagaag
     3421 ccttgatggt gatcttctgc tctttaccgc tgtttttatc tttcgcggaa acgtgcagga
     3481 taccgtcagc atcgatatcg aaggtaactt cgatctgcgg catgccgcgc ggtgccgggt
     3541 tgataccatc taggttgaac tgacccagag atttgttatc agccgcacgt ttacgttcac
     3601 cctgcagcac atggatggtt accgcagact ggttgtcttc agcggtagag aacacctggc
     3661 tgtgcttggt cgggatagtg gtgtttttcg cgatcagcgt cgtcatcaca ccgcccatgg
     3721 tttcgatacc cagagacagc ggggtaacgt ccagcagcag tacgtctttt acgtcaccag
     3781 tcagaacacc accctgaaca gcagcaccga ttgctacagc ttcgtccggg ttaacgtctt
     3841 tacgcggctc tttaccaaag aactcagcaa ctttcttctg aaccattggc atacgagtct
     3901 gaccaccaac gaggataacg tcgtcgatat cagatacgga caggccagca tcctgcagtg
     3961 caactttcag cggctcaatg gaacggttta ccagatcttc aaccaggctt tccagtttcg
     4021 cacgagtcac tttgatgttc atgtgtttcg gaccggtcgc gtctgcagtg atgtatggca
     4081 ggttaacgtc ggtctgctga gcggaagaca gttcgatttt cgctttttct gccgcttctt
     4141 tcaggcgctg cattgccagc ggatcgttgc gcaggtcaat gccctgatct ttcttgaatt
     4201 cttcaaccag atagttgatc agacggctgt cgaagtcttc accacccagg tgggtatcac
     4261 cgttggttgc cagaacttcg aaggtttttt cgccgtcaac ttcgtcgatt tcgataatag
     4321 aaatatcgaa agtaccaccc cccaggtcat aaaccgcgat agtacggttg ccagtgcctt
     4381 tgtccagacc gtaagccagc gcagctgcgg tcggttcgtt gatgatacgt tttacttcca
     4441 gaccagcgat acggcctgcg tctttggttg cctgacgctg agcatcgtta aagtatgccg
     4501 gtacggtgat aacagcttca gttaccggtt cacccaggta atcttcagcg gttttcttca
     4561 tttttttcag cacttcagca gaaatctgcg gcggtgccat tttctggcct ttaacttcga
     4621 cccatgcgtc gccgttatca gcagcaataa ttttgaacgg catgatggaa acatcacgct
     4681 gtacttcttc gtcctggaag cggcggccaa tcaggcgttt aatcgcaaac agggtgtttt
     4741 gcgggttcgt cactgcctga cgtttagccg gctgaccaac cagagtttca ccatcctggg
     4801 tataggcaat gatagaaggc gtggtgcgat cgccttcggc gttctccagc acgcgaggaa
     4861 tggtgccatc cataatcgct acacaagagt tggtagtacc caggtcgata ccaattattt
     4921 tacccatcta aacgtctcca ctatatattc ggtcatcatg tggttgtgag tctgtaatag
     4981 gggcgaaacg tctggtttca actgcccaat ttcatttttt tgcagactca ctcactgcgg
     5041 ttgactacta aatggggtcg taacccacgt catcaagggg gagatgcaaa aattttttgt
     5101 gcttttgcgc agaaatttcg cgatatggca ggacaagacg taaaattgac agcaatttta
     5161 ttcatataaa gaatgaatcg cagccaaggc gttcatcaaa aaattgtaat aaaaagaaaa
     5221 gattacgtgc ctgaatcttc tctttatcag cagtaaacta gtgggtattc atccccctac
     5281 ctcttcccac taagagaatc cttatgaaat ccgtttttac gatttccgcc agcctggcga
     5341 ttagcctgat gctgtgctgc acggcgcagg caaacgacca taaactcctc ggcgtcattg
     5401 caatgccgcg taacgaaacc aacgatctgg cgctgaaact tcctgtttgt cgcattgtga
     5461 aacgcataca actctctgcc gaccatggcg atttacagtt aagtggtgca tcggtttatt
     5521 tcaaagccgc ccgtagcgcc agtcagagcc tgaatattcc ttcagaaata aaagaagggc
     5581 aaaccactga ctggatcaac attaacagcg ataacgacaa taaacgctgc gtctcaaaaa
     5641 tcaccttttc cggtcatacg gtgaactcat cggatatggc cacgctgaaa attatcggcg
     5701 acgactaacg ccagaatata gaagccacaa aaaatgaatg ttaattacct gaatgattca
     5761 gatctggatt ttcttcagca ttgtagtgag gaacagttgg caaatttcgc ccgattgctc
     5821 acccataatg aaaaaggcaa aactcgcctc tccagcatac tgatgcgcaa cgaactgttt
     5881 aaatcgatgg aagggcatcc cgagcaacat cgccgcaact ggcagctgat tgccggagaa
     5941 ttacagcatt ttggtggcga tagtatcgcc aacaaactgc gcggacacgg taaattgtat
     6001 cgggccattt tgctcgatgt ttcaaagcga ttgaagctga aagccgacaa agagatgtct
     6061 acgtttgaaa ttgagcaaca gttactggaa caatttctgc gtaatacctg gaagaaaatg
     6121 gacgaggaac ataagcagga gtttctgcac gcggtcgatg ccagggtgaa tgagctggaa
     6181 gagctgctgc cgctgctgat gaaagacaaa ttattggcaa aaggtgtgtc gcatttgctt
     6241 tccagccaac tgacccgcat tttacgcacc cacgcagcaa tgagcgtact tgggcatggt
     6301 ttgctgcgcg gcgcggggct gggaggccct gtgggcgcag cattaaatgg ggttaaagcg
     6361 gtcagcggca gcacctatcg cgtgacgatt ccagccgtac tgcaaatcgc ctgcctgcgc
     6421 cggatggtta gcgccactca ggtctgatac cggaaaccgg ggaaaatttt cattttcccc
     6481 tcaaaagatc gtagacactg ccccactggc tgattattat gccgcgccct gaaaacacta
     6541 cagttattca gggaaattat ttcaccattc attcgatgat gatttttgag gaattatggg
     6601 caacactaag ttggctaatc cggcaccgct gggcctgatg ggcttcggca tgaccaccat
     6661 tctgcttaac ctgcacaacg tgggttattt cgctctggac ggtattattc ttgccatggg
     6721 cattttctac ggcggcatcg cgcaaatttt tgctggtctg ctggagtaca aaaaaggcaa
     6781 cactttcggt ttaaccgcat tcacctctta cggttctttc tggctgacgc tggttgcgat
     6841 tctgctgatg ccgaaactgg gtctgaccga tgcgccaaat gcacagttcc ttggtgtcta
     6901 cctgggtctg tggggcgtat ttacgctgtt tatgttcttc ggcactctga aaggcgcgcg
     6961 cgtcctgcaa ttcgttttct ttagcctgac cgtgctgttt gccctgctgg cgatcggtaa
     7021 cattgccggt aatgccgcga tcatccactt tgccggctgg attggtctga tctgcggtgc
     7081 cagcgcaatc tatctggcga tgggtgaagt gctgaacgag cagtttggtc gcaccgttct
     7141 gccgattggt gaatcccact aattgtcttg agatcccccc gctcgggggg atttttttat
     7201 tcgctaacgt cgcgtcttgc actcttcggt ctgaatgcag caaccacttc tggtgccgtt
     7261 tcaacgtatg gcccttccag caactgaatg cagtacggta cgctggcaaa aataccatgc
     7321 accacaacgt taccctcagc gtccttcaca ccttccagcg tctctttaat agacttcggc
     7381 tgaccgggta agttaaggat cagcgcctgt ttgcgaatca cgcccacctg acgcgaaagg
     7441 atcgcagttg gtacaaaatg caggctgatc tggcgcatct gttcaccaaa gccaggcatc
     7501 tcgcggtccg ctactgccag cgtcgcatcg ggcgttacgt cacgacgcgc cgggccagtt
     7561 ccgcccgtgg tgagcaccag atggcaactc atttcatcca ccagctcaca caacgtttgc
     7621 tcgatgatcg cctgctcatc ggggattaag cgggtttcca gttcaaacgg cgtggttagc
     7681 gccgatgtca gccattcttc cagcgctgga atgcctttat cctgataaac gccgctggat
     7741 gcgcgatcag agatggaaac taagccaata cgtaaagtat tcatcaagat ttttccgttt
     7801 aaacaatctc gttaaacaga atgatacact gcgaagggag tgacagacag gcttcagaag
     7861 aggtagcgtg accgacttcc cggtcacgct aagaatgatt acagcagatc gccgatcatt
     7921 ttttccagtt tttcctggtc aatagcaaac ttacggatac cttccgccag tttatctact
     7981 gccattggat cctggttgtg ctgccacagg aactcggact cagtgatacg cgccggacgc
     8041 gctttcactt cgccggtgta agacagttta cgttcgatag ccccttcgct ctccgccagc
     8101 tctttcagca gtgccggtgc gatggtcaga cggtcacagc ctgccagttc cagaatttcg
     8161 ccgatgttac ggaagcttgc gcccataacc acggtttcat aaccgtgctc tttgtagtac
     8221 tggtagattt cagatacaga aaccacgccc ggatcttctg ccggagcgta ctctttctta
     8281 tcggtattcg ctttgtacca gtcaagaata cggccaacaa acggcgagat caggaacacg
     8341 cccgcctccg cacaagcacg agcctgagcg aaggagaaca gcagggtcag gttacagttg
     8401 ataccttctt tttccagctg ttctgcagca cggataccct gccaggtaga agccagtttg
     8461 atcagaatac gatcgttgct aatacctgca tcgttgtaga gtttgatcag gcgttttgct
     8521 ttcgcaattg acgcttcagt gtcataggaa agacgcgcat caacttcagt tgagatacgg
     8581 cccggaacca gtttcaggat ttccagacca atatttactg ccagtttgtc ggtcgcgtcc
     8641 acgatctgct gcgcgcgatc gttgctctgc tgtttcgccc aggcgacagc atcatcaatc
     8701 aacttacggt attccggaat ctgcgctgcg ttaagaatga gagaagggtt ggttgtggca
     8761 tcctgcggtt gatacagctt cattgccgcg atgtccccag tgtcggccac tacggtggtg
     8821 tactgacgaa gggaggtcaa tttgtccgtc atgatagtat ttctctttaa acagcttgtt
     8881 agggggatgt aaccggtctg ccctgatgat atcacgacgc aacgtcagcg caaccgccga
     8941 caagacggtt atcgccaggc gagactgttt cggatttctg acactgcgtt gatatcgccc
     9001 accattttta tacaaaacct catgtatgct acgcagaagt tatcaagtac ctcgtagcgt
     9061 atatacttct taaacaattg gtaacgttta cacaggaaag tcatcgcgac cggcaataag
     9121 agggatatgc atgccagatt ttttctcctt tattaacagc gtcctttggg gatcggtaat
     9181 gatttacctg ctcttcggcg caggttgttg gttcactttt cgcaccggat ttgtgcagtt
     9241 tcgctacatc cgccagtttg gcaaaagtct taaaaatagc attcatccac agccaggcgg
     9301 tttaacctca tttcagtcat tgtgtaccag tcttgcggcg cgcgtgggta gcggcaacct
     9361 ggccggcgtt gcgctggcaa ttaccgccgg tggaccaggt gccgtctttt ggatgtgggt
     9421 tgctgcgttt atcggcatgg cgacctcgtt tgccgaatgt tcccttgcac aactttataa
     9481 agaacgtgac gtcaatgggc agtttcgtgg cggaccggca tggtatatgg cgcgcgggct
     9541 ggggatgcgc tggatgggcg ttctgttcgc cgtctttttg ctcatcgcct acggcataat
     9601 tttcagcgga gttcaggcga acgccgttgc ccgagccctg agtttttctt ttgattttcc
     9661 tccgctggtg acgggcatta ttctcgctgt ctttgctctg ctggcaatca ctcgtggtct
     9721 tcatggcgtc gcccggctca tgcaggggtt tgtcccgttg atggcgataa tctgggtact
     9781 gaccagcctg gtgatttgcg taataaatat cgggcaactt ccccacgtca tttggtctat
     9841 ttttgagagt gcttttggct ggcaggaagc ggcaggcggc gcggcgggat ataccttaag
     9901 ccaggcgatt actaacggtt ttcagcgcag tatgttttcc aatgaggtgg gaatgggatc
     9961 gacgccaaac gcggcagcgg cagctgcatc ctggcctccg catccggcag cacaagggat
    10021 tgtccagatg attggcattt ttatcgacac cctggtcatc tgtacggcaa gcgccatgct
    10081 gatattactg gcgggtaacg gcacaaccta catgccgctg gaaggtattc agcttatcca
    10141 gaaggcgatg cgggtgctaa tgggttccta gggtgctgaa tttgttaccc tcgtggttat
    10201 tctgtttgcc ttcagctcca tcgttgccaa ctacatttat gccgaaaaca atctcttctt
    10261 tttacgcctg aacaacccta aagcgatctg gtgtttgcgg atctgcacct tcgcaacggt
    10321 catcggcggc accttgctaa gccttccgct gatgtggcaa ctggcagata tcataatggc
    10381 ctgcatggct attaccaatt tgaccgccat tttactgctc tcgcctgtgg ttcataccat
    10441 tgccagtgat tatctacgcc agcgtaaact cggcgtgcgc ccggtgtttg atccgttgcg
    10501 ttatccggat atcggtcgcc agctttctcc ggacgcgtgg gatgatgttt cgcaggagta
    10561 atcacaacta tcgatcaact cattctcttt ttttgctaaa gtcggcataa atttcctgca
    10621 aggactggat atgctgattc ttatttcacc tgcgaaaacg cttgattacc aaagcccgtt
    10681 gaccaccacg cgctatacgc tgccggagct gttagacaat tcccagcagt tgatccatga
    10741 agcgcggaaa ctgacgcctc cgcagattag cacgctgatg cgcatcagcg acaaactggc
    10801 gggtatcaac gccgctcgct ttcatgactg gcagccagat ttcacgccgg cgaatgcccg
    10861 ccaggcgatt ctggcgttta aaggtgatgt ctacaccggc ttgcaggccg aaaccttcag
    10921 cgaagacgat ttcgattttg cccaacagca tttgcgaatg ctttccggct tgtatggcgt
    10981 actgcgcccg ctcgatttaa tgcagcctta tcgtctggaa atggggatcc gtcttgagaa
    11041 tgcccgaggg aaagatctgt atcaattctg gggagatatc atcaccaaca agctgaacga
    11101 ggcgctcgca gcacaaggcg ataatgtggt gattaatctg gcgtcagatg agtattttaa
    11161 atcggtgaag ccgaagaaat tgaatgccga gattatcaag ccggtgttcc tcgatgagaa
    11221 gaacggcaaa tttaagatca tcagcttcta cgctaagaaa gcacgcggtc tgatgagtcg
    11281 tttcattatt gaaaatcggc tgaccaaacc agagcaactg actggtttta atagcgaagg
    11341 ttacttcttt gatgaagatt cctccagcaa tggcgaactg gtgtttaaac gctacgagca
    11401 gcgttaagtt tgtagtcaat gccggatgcg gcgtgaacgc cttatccggc ctacaaaagc
    11461 atgcagattc aatatattgc agaattaacg taggcctgat aagacgcgcc agcgtcgcat
    11521 caggcgctga atgccggatt gttacccggc atttgtcatt tagcggtgat gtttgcctgg
    11581 accatgaccg ccgtgatgat catgaggagc tttcttatgg tggcgcggcg gtggcggcgg
    11641 tccgtgtggg tgccagcgat tgcctcgcca ttcataatgt tgtttccacc agccgtggtc
    11701 gcgccagtga cctccatccc agtaatagcc acgattatca cgatcgccta tctgtaattt
    11761 tactgacggg actaacgtaa tttccgcagc ctgtgctgcc atgggagcga ccagaaccag
    11821 ggaaagtgcg agtacgatag attgcatctt tttcacaggt tactcctttt atcattgccc
    11881 gttatgggtc gatatccgga gattacgcga tgagaacgac ggttgttatt ctccgcaatc
    11941 ctaatctcta agttaccgca tttattgata atttctcctt tttccctgtc gtttttctcc
    12001 ataatttctt cataaaaaag ccgggctgca taaaagcaaa cccggcctga ttgagataat
    12061 gaatagattt tactgatgat tcatcatcaa tttacgcaac gcagaaaaat cggcgggcag
    12121 attatgtgaa agcaagggta aatcagcacg ttctgccagc tcttttggca gatccaacgt
    12181 ttcaccgaga atcgcttcca cgctctcttt aaatttcgcc ggatgcgcgg tgccgaggaa
    12241 caagccatat tcgcctggat tcaactggtc acgcagcgca cgatacgcta cggcagcgtg
    12301 cggctccgag gtgtagccca gttcttttaa ctcacgcatt gtctgttgcg tggtttcatc
    12361 atccacggct gcataaccca gctctttcag ttgccagatt ttgcggcgga acaactcttc
    12421 cacacgcggc cagttgttcg gctgactcac gtccatcgcg ttggataacg tcgcctgagt
    12481 cgctttgggt gaccactgac cgtcgtgcag gaaacgtggc acggtatcgt tcacgttggt
    12541 cgcagcaata aaacgtttca ccggcagacc gaatgacttc gccagcagac ccgccgtcaa
    12601 atcgccgaag tttccgcttg gcaccgagac aaccagctgg ttgcgcgcct cctgcggcag
    12661 ctgcgcaaca gcttcaaagt agtagcaaat ctgcgccagc aaacggctga tgttaatcga
    12721 gttagccgag tttaacccca gcgccacttt cagttcttca tcatcaaacg cctgcttcac
    12781 cagcgcctga caggcatcga aatcgccgtc gatggcaaca gtttcgatat tgccgcccaa
    12841 cgtacagaac agtttttctt gcagtggact gattttgcct cgtggataga ggataaccac
    12901 tttcacattc ggcaaaccgt agaaagcatg agccactgca gctccggtat caccggaggt
    12961 cgcggtcaga atggtcactg gcttatcatc cgcaatatgg gtcagcattt gtgccataaa
    13021 gcgaccgccg aaatctttaa atgccagcgt tggcccgtgg aacaattcca gacaaccgac
    13081 atcgctttca acattggcga ccggagccgg gaaggcaaac gccgcgcgca cgcgctcttc
    13141 caggatttcc tgtgggattt catcaccaat aaacgccgag aggatcttcg cactgcgggt
    13201 gacaaaatcc agcttcagca tctcatcaat ttcagtcagg ctgaattccg gcaggtcgtg
    13261 cggaaaaaac agcccctgat ttttgcccaa cccctgggtt acggcttgcg caaagctgac
    13321 ctgctcgttg tgatctttca gattgtagag tttcatttag ttttccagta ctcgtgcgcc
    13381 cgccgtatcc agccggcaaa tatgaacaaa accttcctga ttttgcaggt agttcttacc
    13441 caaccagtcg gcaacgcgct gggcggtatc cggcttgtca catagagcga acaaggtcgg
    13501 gccggagccg gagataccgc tcgctaccgc gccgatttcc gcgaccgcct gccgcgcctg
    13561 ccggaagcca ggcagtaacc gttcacggta gggttcagcg ataacatctt tcatcagctt
    13621 cgcggcaagc tcaggctgac gggaatagca ggcgtgaatg aagccagcca gatgtcgccc
    13681 gtgcgcaatg caatcctggc ggcgatactg cgccggtaaa atagcccggg cttctgccgt
    13741 cgagacttta attcccggat acgccagcac ccacagccac tcatcaaacc ctggcacttg
    13801 ctggctgatg atgtcgtttt cttcgatcat caactgcata ccaccaagaa aacacggtgc
    13861 cacgttgtcg taatgaatgc tgccggagat acgtccttcc agctcgccca tcaaagccag
    13921 caaacgagtg tcattaagcg gcttgccgca gtgttcattc atcgccatca gcgccgcgac
    13981 caccgaacag gcgctggagc ctaagcccga accgatcggc atattctttt ccagagtcat
    14041 cgccactgga atttgcttgc caagctcctg gcaaaaacgc tcccagcact gataaacgat
    14101 attttcccgt ggttctgacg gcagcttatc ggcaaagcgt ccgagtttgt tgagactgaa
    14161 tgtctctgcc gcctcaaccg tgactacatc tccgagcaat gcaccatcaa cgggtgccac
    14221 cgccgccccg agcacatcaa acccgacgct catattggca ctggaagccg gggcataaac
    14281 tttaaccatg tcagactcct aacttccatg agagggtacg tagcagatcg gcaaagacac
    14341 cggcagcggt aacgtcattg cccgcaccat atccgcgcag caccaacggc agcggctgat
    14401 aatagtggct ataaaaggcc agggcgtttt cgccattttt cactttgaac agcggatcat
    14461 tcccatccac ttcggcaatc ttcacgcggc agacaccatc ttcatcaata ttgccaacat
    14521 agcgcaaaac ttttccttca tcacgggcct tcgccacgcg cgcggcaaag agatcgtcga
    14581 gctgtgacag attcgccata aaagcggcaa catcaccctc ggcgttaaac tctgcgggca
    14641 gcacaggttc aatttcaata tccgccagct ccagttcacg tcccgtttca cgagcgagaa
    14701 tcaatagttt acgcgccaca tccataccag aaagatcatc tcgcgggtcc ggttcggtat
    14761 aacccatttc ccgcgccagc gtggtcgcct cggagaaact catgccttcg tctaacttgc
    14821 cgaagatata agaaagcgaa cctgaaagaa tgccggagaa cttcaccaat tcatcacctg
    14881 cattgagcag attttgcagg ttctcaataa ccggtaatcc agccccaacg ttggtgtcat
    14941 agaggaattt acgccgcgat ttttccgccg cataacgcaa ctgatgatag taatccatcg
    15001 acgaggtgtt ggcctttttg ttcggcgtga caacgtggaa accttcgcgc aggaagtcgg
    15061 catattgatc cgccactgcc tggctggagg tgcagtcaac aatgaccggg ttcagcagat
    15121 gatattcttt cacgaggcga attaagcgcc cgagattaaa cggctctttg gcttgtgcaa
    15181 gttcttcctg ccagttttcc agattaaggc catgcacact agtgagcaga gccttcgagt
    15241 tggcaacgcc gcagacacgt aagtcgatat gtttattctt cagccagctt tgctgacgct
    15301 tcagttgctc cagcagcgca ccgccaacgc caccgacgcc aatcacaaac acttcgataa
    15361 cctgatcggt attgaacagc atctgatgag taacgcgcac gccagtggtc gcatcatcgt
    15421 tatttaccac gacagagatt gagcgttcag aagatccctg agcaatggcg acaatgttga
    15481 tattggcgcg ggccagcgcg gcaaagaatt tcgccgagat cccacgcaag gtgcgcatac
    15541 catcacctac caccgagata atggccagcc gttccgtcac cgccagcggc tccagtaagc
    15601 cttctttcag ttccaggtag aactcttcct gcattgcccg ttcagctcgc acacagtcgc
    15661 tttgtggaac gcagaaactg atactgtatt cggaagatga ttgcgtaatc agcaccacgg
    15721 aaatacgggc gcgtgacatt gcagcaaaga cgcgcgccgc catgccaacc atccctttca
    15781 tccccgggcc ggaaacgctg aacattgcca tgttattcag attggaaatg cccttgaccg
    15841 gtaattcgtc ttcatcacgg ctggcaccaa tgagcgtacc tggtgcttga ggatttccgg
    15901 tatttttaat caggcaaggg atctggaact gggcgatggg ggtaatggtg cgggggtgaa
    15961 gaactttagc gccgaagtag gaaagctcca tcgcttcctg gtaggacatc gacttcaaca
    16021 acctcgcatc gggcacctga cgcgggtcgc aggtatagac cccgtcaacg tccgtccaaa
    16081 tctcgcaaca atcggcgcgt aaacaggcag ccagcaccgc cgcggagtag tcggaaccgt
    16141 tacgtccaag caccaccagt tcgccttttt cattaccggc ggtgaaacct gccatcaaca
    16201 ccatgtgatc agccggaatg cggcttgccg caatacggcg ggtggactca gcaatatcga
    16261 cggtagattc gaggtaatgc cccactgcca gcagtttttc gaccggatcg ataacggtaa
    16321 cgttgtgacc acgcgcttct aacacgccgg ccataatggc gatcgacatt ttctcgccac
    16381 ggcaaatcag cgcagcgttg atgctatccg ggcactgccc caacaaacta atgccatgca
    16441 ggacatgttt tatttgggca aattcctgat cgacgaaagt tttcaattgc gccagcggga
    16501 accccggctg ggcggcggcg agtcccgtca aaagttcggc aaaaatacgt tcggcatcgc
    16561 tgatattggg taaagcatcc tggccgctaa tggttttttc aatcatcgcc accaggtggt
    16621 tggtgatttt ggcgggggca gagaggacgg tggccacctg cccctgcctg gcattgcttt
    16681 ccagaatatc ggcaacacgc agaaaacgtt ctgcatttgc cactgatgta ccgccgaact
    16741 tcaacactcg catggttgtt acctcgttac ctttggtcga aaaaaaagcc cgcactgtca
    16801 ggtgcgggct tttttctgtg tttcctgtac gcgtcagccc gcaccgttac ctgtggtaat
    16861 ggtgatggtg gtggtaatgg tggtgctaat gcgtttcatg gatgttgtgt actctgtaat
    16921 ttttatctgt ttgtgcgcta tgcctatatt ggttaaagta tttagtgacc taagtcaata
    16981 aaattttaat ttactcacgg caggtaacca gttcagaagc tgctatcaga cactcttttt
    17041 ttaatccaca cagagacata ttgcccgttg cagtcagaat gaaaagctga aaaatactta
    17101 ctaaggcgtt ttttatttgg tgatattttt ttcaatatca tgcagcaaac ggtgcaacat
    17161 tgccgtgtct cgttgctcta aaagccccag gcgttgctgt aaccagtcga ccagttttat
    17221 gtcatctgcc actgccagag tcgtcagcaa tgccatgact cgttcgcgta aagcttgcag
    17281 ttgatgttgg tctgccgttg tgtcactttt cgccggttgt tgtattaatg ttgctaattg
    17341 atagcaatag accatcaccg cctgccccag attgagcgaa ggataatccg ccaccatcgg
    17401 cacaccagta agcacgtcag ccaacgctaa ctcttcgtta gtcagcccgg aatcttcgcg
    17461 accaaacacc agcgcggcat ggctcatcca tgaagatttt tcctctaaca gcggcaccag
    17521 ttctactggc gtggcgtagt aatgatattt cgcccgacta cgcgcagtgg tggcgacagt
    17581 gaaatcgaca tcgtgtaacg attcagccag tgtcgggaaa actttaatat tatcaataat
    17641 atcaccagat ccatgtgcga cccagcgggt ggctggctcc aggtgtgcct gactatcgac
    17701 aatccgcagt tcgctaaaac ccatcgtttt cattgcccgc gccgctgccc caatattttc
    17761 tgctctggcg ggtgcgacca ggataatcgt tatacgcata ttgccactct tcttgaccaa
    17821 ataaccgcga acagggtgat cactgtcaac ttattacgcg gtgcgaattt acaaattctt
    17881 aacgtaagcc gcagaaaaag ccctttactt agcttaaaaa aggctaaact attttctgac
    17941 tgcactaacg gtttaattgt taaaaaatgc tacatatcct tctgtttact taggataatt
    18001 ttataaaaaa taaatctcga caattggatt caccacgttt attagttgta tgatgcaact
    18061 agttggatta ttaaaataat gtgacgaaag ctagcattta gatacgatga tttcatcaaa
    18121 ctgttaacgt gctacaattg aacttgatat atgtcaacga agcgtagttt tattgggtgt
    18181 ccggcccctc ttagcctgtt atgttgctgt taaaatggtt aggatgacag ccgtttttga
    18241 cactgtcggg tcctgaggga aagtacccac gaccaagcta atgatgttgt tgacgttgat
    18301 ggaaagtgca tcaagaacgc aattacgtac tttagtcatg ttacgccgat catgttaatt
    18361 tgcagcatgc atcaggcagg tcagggactt ttgtacttcc tgtttcgatt tagttggcaa
    18421 tttaggtagc aaacatgcag accccgcaca ttcttatcgt tgaagacgag ttggtaacac
    18481 gcaacacgtt gaaaagtatt ttcgaagcgg aaggctatga tgttttcgaa gcgacagatg
    18541 gcgcggaaat gcatcagatc ctctctgaat atgacatcaa cctggtgatc atggatatca
    18601 atctgccggg taagaacggt cttctgttag cgcgtgaact gcgcgagcag gcgaatgttg
    18661 cgttgatgtt cctgactggc cgtgacaacg aagtcgataa aattctcggc ctcgaaatcg
    18721 gtgcagatga ctacatcacc aaaccgttca acccgcgtga actgacgatt cgtgcacgca
    18781 acctgctgtc ccgtaccatg aatctgggta ctgtcagcga agaacgtcgt agcgttgaaa
    18841 gctacaagtt caatggttgg gaactggaca tcaacagccg ttcgttgatc ggccctgatg
    18901 gcgagcagta caagctgccg cgcagcgagt tccgcgccat gcttcacttc tgtgaaaacc
    18961 caggcaaaat tcagtctcgt gctgaactgc tgaagaaaat gaccggccgt gagctgaaac
    19021 cgcacgaccg tactgtagac gtgacgatcc gccgtattcg taaacatttc gaatctacgc
    19081 cggatacgcc ggaaatcatc gccaccattc acggtgaagg ttatcgcttc tgcggtgatc
    19141 tggaagatta atcggcttta ccaccgtcaa aaaaacggcg ctttttagcg ccgtttttat
    19201 ttttcaacct tatttccaga tacgtaattc atcgtccgtt gtaacttctt tactggcttt
    19261 cattttcggc agtgaaaacg cataccagtc tatattacga gtcacaaaca tcatgccggc
    19321 cagcgccacc accagcacac tggttcccaa caacagcgcg ctatcggcag agttgagcag
    19381 tccccacatc actccatcca gcaacaacag cgcgagggta aacaacatgc tattgcgcca
    19441 gcctttcaat accgcctgca aataaatacc gttcattaac gccccaatca gactggcgat
    19501 tatccatgcc acggtaaaac cgatatgttc agaaagcgcc agcaagagca aataaaacat
    19561 caccaatgaa agccccacca gcaaatattg cattgggtgt aaacgttgcg cggtgagcgt
    19621 ttcaaaaaca aagaacgcca taaaagtcag tgcaatcagc agaatggcgt acttagtcgc
    19681 ccggtccgtt aattggtatt gatcggctgg cgtcgtcact gcgacgctaa acgcggggaa
    19741 gttttcccag ccggtatcat tgcctgaagc aaaacgctca ccgagattat tagcaaacca
    19801 gctgctttgc cattgcgcct gaaaacctga ctcgctagct tcccgtttgg ctggtagaaa
    19861 atcacctaaa aaactgggat gcggccagtt gctggttaag gtcatttcgc tattacgccc
    19921 ggcaggcacc acagaaagat cgccggtacc gcttaaattc agtgccatat tcagctgcag
    19981 gttctgcttc cgccagtccc cttcgggtaa agggatatgc acgccctgcc cgccttgctc
    20041 taacccggta ccgggttcaa tggtcagtgc cgttccgtta acttcaggcg ctttcaccac
    20101 accaatacca cgcgcatccc cgacgctaat cacaataaag ggcttgccta agatgatatt
    20161 tggcgcgttg agctcgctaa gacgcgaaac atcgaaatcg gcttttagcg ttaaatcact
    20221 gtgccagacc tgaccggtat aaatccctat cttgcgttct tccacgttct gattgccatc
    20281 aaccatcaat gactcaggta accaaaaatg gataaaactt cgtttccgct ccacggtttt
    20341 atcctcttcc tgcaccgtat aaagctcggt cacaggaata gcgataagcg gcccaacgag
    20401 tttttgcggc ccgctggtac tttggcgaat cgcatcttcc acatcgctac ggtaatcggc
    20461 acgttcgaca atcacttgcc gaatcagcat tatcggaatc aacaacagca atactgcacc
    20521 aaacaaggtg gtcattttcc agaacagggg ggatttcaac atagtcttct cctttgcaat
    20581 ggcagcagga tacgaaggct atgtgggaag aatttgaagc tatgtgaagt gacggtgaag
    20641 tcgtagcgag gccagcacgc caccttcctg cacgttgcgc agcgtgactt cgccgttaaa
    20701 caaacgggcg acctcactga cgaacgccaa ccccagaccg ctgctttttt gcccatttgc
    20761 acgcggcaaa gagtaaaagc gttcaaaaat acgtgacagc gcgtagtcag gaataccact
    20821 accggtatcc agcaccttaa gcgtgacgtg ttcctgatcc acttcggcgc ttagcgttat
    20881 gcaaccgctc tcgggagtaa aatcgatggc gttatccagt aaattcccca gcgcctgatc
    20941 cagtaacgcc ggttcagcag caacattaac ctcggtgggc ataacatgca aagtgatgtt
    21001 tttttctgcc aactgcacgg tgcgcgcttc gctgacgcgg cgaaataatg ccgccacatc
    21061 aacagcagtc agaacgactt cctgacgatt ctccagtctt gcctggcgta gtaacgtttc
    21121 caccagcgcc tgcattcgcg cattttgtgt cagaatgttg tcagtaaaac gagccactac
    21181 ttcaggcggc ggaccttcgc gtaaaatttc cgccgcgcca cgaatcgccg ccagtgggct
    21241 ttttagctca tgggttaacg cataaacata ctgctcaata tagtttttcc cttccagctt
    21301 cacgcgcata ctttccagcg cctgcgcgag tttacgcagc tcgctactac cgagatcggg
    21361 aagaggaacg ggcttattgt cagtgacgga atcagcatag cgagtgagcc gggcaatgga
    21421 gcggttgatc caccaaacca tgcctgcgcc aatcaccagt gcaatcccca acaaaatggc
    21481 gctggcccat aaaattcgcc gctcgctgcg tttaatgacc ggagccatcg ccgcgttcgg
    21541 tttgcctacg ctcaaaacgc caataagccg cgagccgccc ataatcggcg cggcaacata
    21601 catcaccgaa ctttcgggat cggcaggatt ttgcaacgtg ctgcgcgcac catactgacc
    21661 acgcaacgtt agccagacgt cattccagcg cgaataatcc tgtccaacgg ctttatttgc
    21721 cgaatcgaac aatactttgc catgcgcatc ggtcatatag acatgatatt cattgcgcac
    21781 tttgttaatg ccaccaatat tggcgcgaaa cgggcgatgt tgtagctgat taaacgcctg
    21841 cgccagttgc ccatgcgttg ggtccccaga gagcaaatcg ggacgcgcca gctccgcaag
    21901 taacgttgcc gtgtcgatca acgtcccctc cgttgctctt cgcacgcccg gtttaacttc
    21961 tttgacaaaa atagccagta cgaaccaggc cgccaccgcc accagtaaaa aatagcccag
    22021 cagcaaccgc atgccgatac gcattacaag cccctcaggc tatatcccat gccgcgatga
    22081 gtattaatcg gtgaaagatc ggggttgatg gcgcgcagct tggcacgcag cgttttaatg
    22141 tggatatcga cggtgcgatc gtaggtgtcc tgcgcatctt cccacacgct atccattagt
    22201 tgctggcggg accagacgcg gcctggcgac ttgagtaacg tcttcagcaa taaaaactca
    22261 taccgcgtca gcgttaatgg cgtgtcaaac cagctgatct gcgccgcggg ttcattcaat
    22321 tcaaaatgac caatacggat gacgggagac ggcgtcgaga acttcttcac ccgacgcagt
    22381 aaggtgcgca ccctggcgca cacttcgcgg ggtgaaaacg gtttagccac atagtcgtca
    22441 gcgccaattt ccagcccaag caggcgatcg acctcttcac ttcgggccgt caggaacagt
    22501 acaggtaacg ccggatagag cgccagtaac tggcggcaca attcaaagcc actgatatca
    22561 ggcaaaccaa cgtcgagaat catgacgtcg ggaacctgct ggcgagcttt atccagcacc
    22621 ggcaaacctc gctcaaagac ctcgacggca aaaccttcct gctgcaacat gtagaccagc
    22681 gtgtcggcta tcccttgctc atcttccact aaccagaccg tttcccgttg cataaatcgc
    22741 ctctgttatt gccgccacgg catgacagga accgcgctaa tcgcgttttt cggcgaacct
    22801 tctacaactt tgtcggagta agccagataa gcgagcgcgt tgcgtttggc gtcataaaag
    22861 cgcacgacct gtaacgactt aaagaccagg gacgtgcgtt ttttgaatac tacctcgccc
    22921 tgagctttgc cgtttttaat acgatccgac agttcaatcg gcccgacttg ctgacaagaa
    22981 atggccgcat cggaggtatc ttctgccagg cccaatcccc ctttaatacc accggttttc
    23041 gcccggctca cataacaggt gacatttttc acatcgggat cgtcaaaggc ttccacaaca
    23101 attttgtgat ccgggccgat cattttaaat acggtgtcga ccgaaccaat ctcttctgca
    23161 tgagccaatg gccccagcat tatcaggctt aaagacagga tcaaatgctt gtatttcata
    23221 ttgttaccat ttttatctat tacacgagca attagttcgt cacggtaaat aacatattga
    23281 ttaagaacaa aatctcaatt cttttatttc cgtcaagccc taaaacatac tctactaaga
    23341 aaaaaacact gaatgctaaa acagcaaaaa atgctattat ccaattacct gatgtcaggt
    23401 gctcgttgtt gaaaggatga ggatatttta tggatcaggc cggcattatt cgcgaccttt
    23461 taatctggct ggaaggtcat ctggatcagc ccctgtcgct cgacaatgta gcggcgaaag
    23521 caggttattc caagtggcac ttacagagaa tgtttaaaga tgtcactggc catgctattg
    23581 gcgcgtatat tcgtgctcgt cgtttgtcga aatcggcggt cgcactacgc ctgactgcgc
    23641 gtccgattct ggacatcgcg ctgcaatacc gcttcgactc tcaacagaca tttacccgcg
    23701 cattcaagaa gcagtttgcc cagactcctg cactttaccg ccgttctcct gaatggagcg
    23761 cctttggtat tcgcccgccg ctacgtctgg gtgaattcac aatgccagag cacaaatttg
    23821 tcaccctgga agatacgccg ctgattggtg ttacccagag ctactcctgt tcgctggagc
    23881 aaatctctga tttccgccat gaaatgcgtt atcagttctg gcacgatttt ctcggcaacg
    23941 cgccgaccat tccgccggtg ctctacggcc tgaatgaaac gcgtccgagt caggataaag
    24001 acgacgaaca agaggtattc tataccaccg cgttagccca ggatcaggca gatggctatg
    24061 tactgacggg gcatccggtg atgctgcagg gcggcgaata tgtgatgttt acctatgaag
    24121 gtctgggaac cggcgtgcag gagtttatcc tgacggtata cggaacgtgc atgccaatgc
    24181 tcaatctgac gcgccgtaaa ggtcaggata ttgagcgata ctacccggca gaagacgcca
    24241 aagcgggaga tcgcccaatt aatctgcgct gtgaactgct gattcctatc cgccgttaac
    24301 gctgcagctc atctaatgca ggggcgtcca gatgcgagat gtcccctgcg gtttcaacga
    24361 cccagccaga cgccagccac aggctttcct gataatccac gcgcgaaata gaacagttac
    24421 gcagacgtaa gcggcgctct gcccatgctg gtaatccgag aatcgtactc accagacatc
    24481 ccagtgcaat accatggctc accagcagcg gtcggcttcc ctgcggtaag tcccggcaag
    24541 attccagtgc cgcattgacg cgatcgctga gctcctgcat tgactcgcct tcaggaatac
    24601 gcccgtcaac ggtgccattg accagctgcc gacgccagtt ctcttcttct tcggtcagag
    24661 aatcgatatg tcttttttcc agcacaccca tgtttaattc acgcaggcga gaatcaaaga
    24721 tgatgtcaca gccgcaggcc tgggcgatga tttccgccgt acgccgggtg cgtcctaaat
    24781 cgctactgat aatatgcgta atgccaagct ctttggcacg ggttgccacc tgcatcgcct
    24841 gttgctcacc tttggcggtc agcgggctgt cagactggcc ctgaatacgt cgctcggcgt
    24901 tccactgcgt ttcaccgtgg cggactaggt atacctgtaa catgcttttt ttccgtatac
    24961 tgctgtaacg ttaattttct gagtaatgct gattatgcac caagttgtct gtgcgaccac
    25021 caatcccgct aaaattcagg ccattctgca ggcatttcac gagatcttcg gcgaaggatc
    25081 ctgccatatt gcatccgttg ccgtcgagag cggtgtaccg gaacaaccct ttggcagtga
    25141 ggaaacgcgc gctggcgcac gaaatcgggt agcaaatgcc cgccgtttac ttccagaggc
    25201 tgatttttgg gtggcgattg aagctggcat tgatggcgac agcactttca gttgggtggt
    25261 gattgaaaac gccagccagc gcggcgaggc gcgttcggca actttgccgt tacctgcggt
    25321 gattctggaa aaagttcgtg aaggcgaggc gcttggtccc gtgagtcgcg ttataccggt
    25381 attgatgaga ttggtcgtaa ggaaggggcg atcggcgtgt ttaccgccgg gaaactcact
    25441 cgcgccagcg tctatcacca ggcggtgatc ctggcgctga gtccgtttca caatgccgtg
    25501 tatcaggcat tgtaggacgg ataaggcgtt cacgccgcat ccgacattcg gtgcacgatg
    25561 cctgatgcga cgctgccgcg tcttatcagg cctacaaaat caatcgcttt tcagcaacac
    25621 ctcttccagc cactgacgca gctccacggg cgcggctttc aggctgttag atccacgcgt
    25681 aatcgtcgcg atgcccgcgc cgagttcatt ttttaactca cgctgactca tttcgccacg
    25741 caacagctct tcgacaatac gcacgcgagt ccccaacgct tcgcgctcat ctggcgtcag
    25801 catcaggttt aacaacggta aatggagatc gttttggtag gcattcttaa gcaggtcgac
    25861 aaaacgtaac cactcctggt gacgctgttc tgccatcgct gctgaatagg gtgattgttg
    25921 ggccataata tgtcgccatt gttagcgggg gaagcaaaat gactcccccg gttgtactcg
    25981 ctaaagagta cgatagcata tcataaacgt gcggatcagt aacgacgtcc ccattccgtg
    26041 gcgctcatca acgtcggttt atcccccatg aaatagcggt agtaagcatc ataagccagc
    26101 acgttcttca cataaccgcg cgtctcggag aacggaatac tctcgacaaa tgccacagcg
    26161 tcgatacgcc cggcgctgtt gccaagccag gttcgcaccc gtccaggtcc ggcgttataa
    26221 gctgctgagg agaaaatacg gttattgcca aactgctgat aaacatattg caggtaactg
    26281 gtgccaatgt tgatgttcgt ttccggatcc agcaattgcc cagggctgct ataaccagga
    26341 atagagaaca tcttcaccgt atgggtcgct gtaccaggca taatctgcat caggccgctg
    26401 gcccctaccg gtgatttcac tttcggattc caggcgctct cctgacgggc aatcgccatc
    26461 gcatagcttt gcgggatctc tttcccactg gtgtaacgtt tgaaaagatc gttgtaagcc
    26521 agcgggaatc gctcttccag atgatcccaa agcttcccgg cgatcgttgc ctgaacgcta
    26581 agatcccacc attggttatt gaaagcatac cgagccagtt gagcctgctc tgtttttgac
    26641 ttgctcttca ccagattggc ccactcgcta cgcgcggtat tatcgagatt ccagtacatc
    26701 aactcgcgca cgcgcgccat ctccggcccc tgagtcaggg tgctgtcaac attctgcggc
    26761 gctttatcaa tcttcagctc atactcttca ccgatgcgtt gtgctgcaac catcgggtag
    26821 aaaccacgct gttgtatgag ttgatgcaaa atctctttcg cttcagcttc acgtccgcgt
    26881 tccagcaata aatccgcctg ccagtaacgc cattcatctt tctcttttgc ttccatcggc
    26941 agacgcgcca gccaggtatt caggccgcga cgatcgccgg tgccaagcgc cattcgcaca
    27001 cggcgttcaa tcaacgacgt ggattgcgag cgcataatgg cgtcatcgcg ccatttcgcc
    27061 tgctcatcgg tgacatcatt gcccatcaaa cgccaggcga cgatatcgcg cagctcctga
    27121 atctgatctt cattaagctg ctgcgcctgg gcaagcgacg ggatcatcag ccgtgcattt
    27181 tcggcatcct gccgcgccac actggcaaac gccaccgccg ccatttgacg ggtaaaatcg
    27241 gtcgcgccgg ttgtacgcgc gaaggtcagt accgtattag ggttgttcgc cagtgaaatg
    27301 attgccgagg cgatagtctg gtaatcggca ggcatctgcc ctgccagcac tgttaccagg
    27361 cctgtgttac ccgctttcat cgccagacgg atacgctcta aatacgccag cggatcttgt
    27421 ttacctgacg cacgccagac gctaaataac ttgtcacagg cgttaggctg gctcttgccg
    27481 gttagccaca gctctttcgc cccttgccag gcttcttcac tctgcccggt gttccatttc
    27541 gcatagtagt aattacattg cgcttcggta gttccgggct tttccgggct aaaggctaac
    27601 aagccacgcc agtcttcacg ccgcgccagt tcattgacga aacgagattg cagcgtgcga
    27661 gcgggaggaa gcgtggggtt agcgcgaaca aagttagtga ccgtcaccgc cggttgattc
    27721 atcaggtcat cggtgatctg gcggtattcc aggtagggat aaagcggata atccttcagt
    27781 ccaggcatca tttgttcgac cacatccatc tgtcgattat cccaggcctg cttgatttgc
    27841 gcgtaacggc tacgctgctc atccagtgag tcggctcgcg ccacgctgct gaccgtcagc
    27901 agacagacac cggcagccaa cagccgccag gtaacttgtt tggctttttc cacaagcgca
    27961 tcctctaagt gtaaatacat caatgcagcg tcatgccgcg taatagattt atgctaacca
    28021 gtcattgccg tttacgccac gttacggaca cttttttact tttactgcga aggtgatcga
    28081 ccaactggga ttaggcgggg aaacaggcta cacttcgcct ttgaaaactc atcatcacga
    28141 taaacataga ggcgaagtcc aacgtggctc aattcgttta taccatgcat cgtgtcggca
    28201 aagttgttcc gccgaaacgt catattttga aaaacatctc tctgagtttc ttccctgggg
    28261 caaaaattgg tgtcctgggt ctgaacggcg cgggtaagtc caccctgctg cgcattatgg
    28321 cgggcattga taaagacatc gaaggtgaag cgcgtccgca gccagacatc aagattggtt
    28381 atctgccgca ggaaccgcag ctgaacccgg aacacaccgt gcgtgagtcc attgaagaag
    28441 cggtttcaga agtggttaac gccctgaaac gcctggatga agtgtatgcg ctgtacgccg
    28501 atccggatgc cgattttgac aagctggccg ctgaacaagg ccgtctggaa gagatcattc
    28561 aggctcacga cggtcataac ctgaacgtac agctggagcg tgcggcggat gcgctgcgtc
    28621 tgccagattg ggacgcgaaa atcgctaacc tctccggtgg tgagcgtcgt cgcgtggcgt
    28681 tgtgccgcct gctgctggaa aaaccagaca tgctgctgct cgacgaaccg accaaccacc
    28741 tggatgccga atccgtggcg tggctggaac gcttcctgca cgacttcgaa ggcaccgtgg
    28801 tggcgattac tcacgaccgt tacttcctcg ataacgttgc aggctggatc ctcgaactgg
    28861 accgcggtga aggaattccg tgggaaggta actactcctc ctggctggag cagaaagatc
    28921 agcgtctggc gcaggaagct tcacaagaag cggcgcgtcg taagtcgatc gagaaagagc
    28981 tggagtgggt acgtcaggga actaaaggcc gccagtcgaa aggtaaagca cgtctggcac
    29041 gctttgaaga gctgaacagc accgaatacc agaaacgtaa cgaaaccaac gaactgttta
    29101 ttccacctgg accgcgtctg ggcgataaag tgctggaagt cagcaacctg cgtaaatcct
    29161 acggcgatcg cctgctgatt gatgacctga gcttctcgat cccgaaaggg gcgatcgtcg
    29221 ggatcatcgg tccgaacggc gcgggtaaat cgaccctgtt ccgtatgatc tccggtcagg
    29281 aacagccgga cagcggcacc atcactttgg gtgaaacggt gaaactggca tcggttgatc
    29341 agttccgtga ctcaatggat aacagcaaaa ccgtttggga agaagtttcc ggcgggctgg
    29401 atattatgaa gatcggcaac accgagatgc caagccgcgc ctacgttggc cgctttaact
    29461 ttaaaggggt tgatcagggt aaacgtgttg gcgaactttc cggtggtgag cgcggtcgtc
    29521 tgcatctggc gaagctgctg caggttggcg gcaacatgct gctgctcgac gaaccaacca
    29581 acgacctgga tatcgaaacc ctgcgcgcgc tggaaaacgc cctgctggag ttcccgggct
    29641 gtgcgatggt tatctcgcac gaccgttggt tcctcgaccg tatcgccacg cacatcctgg
    29701 actaccagga tgaaggtaaa gttgagttct tcgaaggtaa ctttactgag tacgaagagt
    29761 acaaaaaacg cacgctgggc gcagacgcgc tggagccgaa gcgtatcaag tacaagcgta
    29821 ttgcgaagta atgcgtaaaa tgccggatgc ggcgcgaacg ccttatccgg cctacaaaac
    29881 cagcaatttc aatacgttgt aaaactgtag gcctgataag acgcggtaag cgtcgcatca
    29941 ggcattgagc accgaatgcc ggatgcggcg tgaacacctt ttccggtcta caacagcctt
    30001 agcatcctca atttcgtggc gggtcacccg tgcaggtttt ttcaaacctt gtttaatctc
    30061 gacagcctct tccagagagg ttttcaactc atcaaaaaaa ctcattttat acctcgcctt
    30121 tgagcagttt cgtcacggtt atccttgctc ccccatcatc tcccgcacca gctccacgca
    30181 gcgcaggaaa cggctgtcgt aatcgtcctc ttcaacccgc acgaattcga tattgttctc
    30241 ttccagcatc tccaccagca agttctggaa ctctttgcga tccaccgaac tgccgaggct
    30301 gcgtaaacca tccgccaccc acggcgtgtt gttctccagc aggatcacca gatcgaaacg
    30361 gtattcatca atcagcgcct gcacgaacgg atgctcacgc ccttcgtact ttttgcagaa
    30421 cgcctgggta gtgacaaaat cggtgtcaat aaacgccact ttattggcgt atttcactgc
    30481 aaaatcaatg tattgtgcgt gacccaacgc gattttatcg tagtcggaat actgcaatgc
    30541 gatttcatca ccgccgaggt gtgaaaagac ataatcgcgg ccatattccc acgcactggt
    30601 ggtgttgaag atattggcaa gtttgtttac cagggtggat ttaccgctcg actcgccgcc
    30661 aaggatcgcc acggtacgca caaaaaacgg cttcacttcg gtaggaatat attcccagta
    30721 gcggaacggg ttttcgcgga tctgcgcacc gctgatactc ataaaggtac gtttcggatc
    30781 gaccagcacc gtctcgatcc ccagatgttc catatactgt ggcgcatcgg cttcttccga
    30841 ggtgtagatc aggtccggct ggatcccctt ttcagccata aactttttga tgccgttgct
    30901 ccacacatcc cagccatgcg gatacggctc catgccctct tcgttgaaag catgaatacg
    30961 gatatttttc tgatatttaa aggtttgcaa tagccagcgc agacgatccg gcaccgtggg
    31021 ctgctgcgac atggcgctgt cttcaaacaa cgcgcggtcg cgggtatcgt caaaacccat
    31081 aatgatatgc agctcgtcaa cctggctaca ggcgcgctgg ataaggtaga tatgtccggt
    31141 atgcagcggg tagaacttac cgaatacgac accaatggtt ttcttctgcc ggggaaattc
    31201 aagccccaaa aaacggtgca atgcctccag cttttgcgcg ctggggcttt taattttggc
    31261 attcagtaac tggcttaaat accctttggt cataccgctg gcatcagcta cctgctgtag
    31321 cgtgcagccc tgttgcttga tggcagtttt caggtaatca aatgacgaca tatctccctc
    31381 cgtatctctc attataagtc gtcgaacacg ctaagcgcgt cggagagttt tttaacgcca
    31441 aaaatctgca tcccttccgg cgcttttttc ggtacgttag ccgccggaac aatcgcccgg
    31501 cgaaaaccgt gtttcgccgc ttcagaaatc cgctcctggc cactgggcac cgggcggatc
    31561 tcccctgcca gcccgacttc accaaacacc accagatcct gtggcagcgg tctgtcacgc
    31621 aggctggaaa ccatcgccag cagtaacgct aagtcggcgc tggtttcggt taccttcacg
    31681 ccgccgacca cgttcacaaa cacatcctga tcggccattt gcagaccacc gtgacggtgc
    31741 aagacagcca gcaggattgc cagacggttt tgttccagcc ccactgccac gcggcgcgga
    31801 ttcgccatta tcgagtgatc gaccagcgcc tgaatctcca ccagcaacgg acgcgttcct
    31861 tcccacacca ccatcacgga gctgccggag gtcacttcat ctccgcgact taagaaaatt
    31921 gccgaagggt tgctgacttc acgcagcccc tgttcggtca tcgcgaagac gcccagctca
    31981 ttcaccgcgc cgaagcggtt tttatggctg cgtaaggtgc gaaaacggga gtcggcatcg
    32041 ccatccagaa gcaccgaaca gtcgatacag tgttccagca ctttcgggcc agccagcgaa
    32101 ccatctttgg ttacgtgtcc aaccatgaca atcgccacac cgcgcgtttt ggcgaaacgc
    32161 gtcagataag ccgccgtttc acgcacctgc gccacgctac caggcgatga ttgtacatcc
    32221 gccatatgca tcacctggat cgagtcaatt accatcagct tcggttgctc ttcttcggca
    32281 atcaggcaga tctgctcgat gctggtttcc gacaacatat tgagattgtc agtcggcagg
    32341 ccaaggcgat gagcgcgcat tgccacctgt tgcagcgact cttcgccggt gacatacagc
    32401 gttttcatct gctgggccag tttgcacagc gtttgcagca acagcgtgga tttccccgca
    32461 ccagggttac cgccaatcag aatggcactt cctggcacca cgccgcctcc tagtacgcgg
    32521 tcgaactctt taaatccggt ggaaaaacgc ggcagctctt caaggctgat atcggagagt
    32581 ttctggactt ttgccacccc ggcgctaccg gcatagccgc tgagacgctc gttacgcgcc
    32641 accgttggcg acgcagcaag acgcacctcg gtgatggtgt tccaggcatg acaggcactg
    32701 cactgcccct gccagcgcgg ataatcggcc ccgcattcat tacaaacaaa ggcgcgtttt
    32761 ggagcttttg ccacggtgta cctcgttaat gctgtgccgc ccgcaggatg acgggcaggc
    32821 aattacttct gattcaggct gccagagagg atgcagaata cccccatcag gtcagcgtga
    32881 cggatggtga cttccgcctt ttcattcact tttggcttgg catggtaggc aatccccagc
    32941 cctgctgctt tgatcatcgg caggtcatta gctccatcgc caatcgccac ggtctgcgcc
    33001 agcgggattt catactcctg cgcgaggcga gtcagagttt tcgctttgta ctgcgcgtct
    33061 acgatgtcgc cgatcacatt gccggtaaat ttaccgtcca tgatctccag ttcattggct
    33121 accacggctg tcaggcgcag cttgtcgcgc aggtattcag caaagaaagt aaagccgccg
    33181 gaggcaatcg ccactttcca gcccagcgtt tccagcttga gcaccagctg cgttaagcct
    33241 ggcatcagcg gcagattttc acgcacctgt tgcagaatat tggcgtcagc gcctttcagc
    33301 gtcgccacgc ggctgcgcag gctggcggta aaatcgagtt cgccgcgcat cgcccgttcg
    33361 gttacctccg ccaccatctc gcccgttccg gccagcttgg caatttcatc aatacattca
    33421 atctggatgg cggtggaatc catgtccatc accagcaaac ccggcgtgcg caggtgcggg
    33481 attttcccca gcggggcgac atccagctgc gcttcgtggg ccaggcgtgt agcccgtgcg
    33541 gtgagtgaac ctgccagacg aatcacctga taatcttcca cgcaccaggc ggcaacaatc
    33601 accatcgccg cacccagttt gctctggtat tgggtcagac gctgtttatc cagcccacga
    33661 ccatacagca gccagccgct acgacctgcg tggtaatcca gtggcatcac ttcatcacca
    33721 cttaatgaaa gaggcagacc cggccataaa gagacatctt caggcaggtc gcaccaggta
    33781 atgttaggca ttaaggctcc tgtaaaatcg ttcgaagcag ggaaaataac gcatgaggct
    33841 accttgtatc cattgcttct ggcaacatta agtctcaaat tttcaaaggg tggaagatgg
    33901 ctcgcacaaa actgaaattc cggctacatc gggcagtgat tgtcctgttc tgtcttgcct
    33961 tgttagtggc actgatgcag ggggcgtcat ggtttagtca aaaccaccag cgacagcgta
    34021 atccacagct ggaagaactg gcccgcaccc tggcgcgtca ggtgacgctg aacgttgcac
    34081 cgctgatgcg taccgactca ccggatgaaa aacgcattca ggcgatcctc gatcagttaa
    34141 cagatgaaag ccgtatcctc gacgcgggtg tgtatgacga acaaggcgat cttatcgcac
    34201 gttctggcga aagcgtcgaa gtgcgcgatc ggctggcgct cgacggtaaa aaagcaggcg
    34261 gctattttaa ccagcagatt gtcgagccaa ttgcgggtaa aaacggaccg ctcggctatc
    34321 tgcgcctgac actcgacacc catacgctcg ccaccgaagc ccaacaggtg gataacacca
    34381 ctaacatttt acgcctgatg ttgctgctct cactggcaat cggcgtagtg ctgacccgca
    34441 cgctgctaca gggtaaacgc acccgctggc agcaatcgcc cttcctgtta accgccagca
    34501 aaccggtgcc ggaagaggaa gaaagcgaga aaaaagagtg acccattact acaagaaagg
    34561 aaatcgttat gtccacatta cgcctgctca tctctgactc ttacgacccg tggtttaacc
    34621 tggcggtgga agagtgtatt tttcgccaaa tgcccgccac gcagcgcgtt ctgtttctct
    34681 ggcgcaatgc cgacacggta gtaattggtc gcgcgcagaa cccgtggaaa gagtgtaata
    34741 cccggcggat ggaagaagat aacgtccgcc tggcgcggcg cagtggcggt ggcggcgcgg
    34801 tgttccacga tctcggcaat acctgcttta cctttatggc tggcaagccg gagtacgata
    34861 aaactatctc cacgtcgatt gtgctcaatg cgctgaacgc gctcggcgtc agcgccgaag
    34921 cgtccggacg taacgatctg gtggtgaaaa ccgtcgaagg cgaccgcaaa gtctcaggct
    34981 cggcctatcg cgaaaccaaa gatcgcggct tccaccacgg caccttgcta ctcaatgccg
    35041 acctcagccg cctggcaaac tatctcaatc cggataaaaa gaaactggcg gcgaaaggca
    35101 ttacttcagt gcgttcccgc gtgaccaacc tcaccgagct gctgccgggg ctcaccatga
    35161 gcaggtttgc gaggccataa ccgaggcctt tttcgcccat tatggcgagc acgtggaagc
    35221 ggaaatcatc tccccggaca aaacgccaga cttgccaaac ttcgccgaaa cctttgcccg
    35281 tcagagtagc tgagagtgga acttcggtca ggctccggca ttctcgcatc tgctggatga
    35341 acgctttagc tggggcggcg tggaactgca tttcgacgtt gaaaaaggcc atatcacccg
    35401 cgcccaggta tttaccgaca gcctcaaccc cgcgccgctg gaagccctcg ccgggcgact
    35461 gcaaggctgc ctgtaccgcg cggatatgct gcaacaagag tgcgaagcgc tgttggttga
    35521 cttcccggac caggaaaaag agctacggga gttgtcgacg tggatagcgg gggcggtaag
    35581 gtaactcgct gattaaaata tccaaggaag gaaatatgat cgatctggaa aaccaggaac
    35641 gggagattat caatttaatg ttcagccagc gtatttcctg gctagccgcc gtgcgtatca
    35701 ggcataaatt atcgcttgct gaagtaagta aaatgttagg tatctcgatt aattctctaa
    35761 aacagatcga aaagacggaa cggcttagct caaacatcaa aagcaaaatg gcagaaattt
    35821 atggttgtcc accagagttg ctgatatgcc cttcctggat gacggctgaa cataaatgaa
    35881 tagattcgtg aatagatact tgaatgtttt ttattttcta tccatatgaa aaataatagt
    35941 aaatctataa atcagttgct attgatgaat agatactaaa aagccggagc agtcccccac
    36001 tccggcttaa cacgacaatc gcctttcagc gaaacacaat tactctttat cgcccagcag
    36061 aacggattcc agtgcgattt tgatcatgtc gttgaaggtg gtctgacgct cagcggcagt
    36121 ggtctgctcg tgagtgcgga tgtggtcaga cacggtgcag atggtcaggg ctttcgcgcc
    36181 aaactctgca gccacgccgt agataccagc cgcttccatt tccacgccga ggatgccgta
    36241 tttttccatc acgtcgaaca tttcgccgtc cggagagtag aacaggtcag cggagaacag
    36301 gttacccacg cgagcatcaa cgcccagtgc tttagctgca tctactgcgt tacgcaccat
    36361 gtcaaagtca gcgatagcgg caaagtcatg gtctttaaaa cggatgcggt taactttgga
    36421 atcggtgcag gcccccatac cgataacgac gtcgcgcagt tttacgtgcg gcagaactgc
    36481 gccacaggaa ccaacgcgga taattttctt cacgccgaaa tcggtgatca gttctttggt
    36541 gtagatggag caggacggga tacccatacc gtgacccatt acggaaattt tgcggccttt
    36601 gtaagtacca gtgaagccca gcataccgcg aacgttgttc acttcacggg catcttcaag
    36661 gaaagtttca gcaatatact tcgcacgcag cgggtcgcct ggcatcaaaa ctacgtcagc
    36721 gaaatcgccc atttctgcat taatgtgtgg ggtagccatt gttttatcct ttttgtgaca
    36781 taacaaaggc ggagtcaacg ctccgcccaa atccatcaga acatggcttt gccatattcc
    36841 atatcagaag taccaaaata ttttgccaga gtctggccga tatccgcgaa ggtttcacgg
    36901 tgacccagtg agcccggttt tactttcggg ccatatacca gtaccggaat gtgttcacgc
    36961 gtgtggtcag taccggtcca ggtcggatcg caaccgtggt cagcggtgag gatcaggatg
    37021 tcgtcatcgc gcagcagaga catcagctcc ggcagacggc ggtcgaacag ttccagaccc
    37081 gcagcataac cggcgacgtc gcggcggtgg ccccaggaag agtcgaagtc aacgaagttg
    37141 gtgaagacga tggtgttatc acccgcttct ttcatctctt tgatggtggc gtcaaacagc
    37201 gcgtccaagc cagtcgcttt cacttttttg gtgataccgc agttggcgta gatgtctgca
    37261 attttaccga cagaaaccac ctggccgtgt ttttcatcaa ccagtttctg cagcacggtc
    37321 ggtgctggcg gctcaacagc caagtcgtga cggttaccgg tacgctggaa gttaccagct
    37381 ttgtcaccga taaacggacg agcgataaca cgaccgatat tgtagccgcc gttggtcagc
    37441 tcttcacggg cgatttcgca cagttcgtag agtttatcca gaccgaaagt ttcttcatgg
    37501 caggcaatct ggaacacgga gtcagcggag gtatagaaaa tcggcttgcc ggttttcatg
    37561 tgctcttcgc ccagttgatc cagaatgacc gtaccggaag agtggcagtt accgaggtaa
    37621 cccggcagat tagcgcgttc gaccagttta tccagcagct cttgcgggaa gctgttttcg
    37681 tgatcggaga aatatcccca ctcaaacaga accgggacac cggcgatttc ccagtgacca
    37741 gacggggtat ctttaccgga tgacatttcg tgcgcccatg cgtacgcgcc gataacttca
    37801 gcgttgccgt ccattcccgc cggaatgaaa ccggtagaac cttcgtgtgc tttcgccagc
    37861 cccagacggg tcagatttgg cagattgagc ggccctttac gaccattatc agcttcgcct
    37921 ttggcacaag cttctgcgat atgacccagg gtgtcagccc cgacgtcacc aaagcgttct
    37981 gcatcttctg tagcgccgat gccgaatgag tccagcacca taataaatgc acgtttcata
    38041 tgttctccgt actcagtgct tcaaatttaa atgatcagat cagtataccg ttattcgctg
    38101 atacggcgat agacagttgg tgtgctttcc ggtgcgttat cggcaagttt aattgccgct
    38161 ttcaccgctt tcgccgcttc ctgccagctg ttttcgtctt tcgcgaggat aaccgccagc
    38221 ggacgctgac cgtctacctg gtcgcccaga cgcgccatat cagtaaagcc gacgctgtaa
    38281 tcgatggtgt cagatgcctg acgacgaccg ccgcccattg caactactgc catccccagc
    38341 gcgcgggtat ccatttcact gacaaaacct tcggtatcag catagactgc tttcgtcagc
    38401 atcgctgtcg gcagatactt cgcgtagttc tcaacgaagt cggtcgggcc tttttgtgcc
    38461 gctaccatac gaccaaagac ttctgccgct ttaccgttgt ccagcaccgc ctgcaatttc
    38521 gcgcgcgctt cggcgtcatc tttcgccagt ttgccggaga tcaacatctc cacgcacagc
    38581 gccatcgtga catcaaacag acgcgggtta cgatactcac ccgtcaggaa ctgcaccgct
    38641 tcacgaactt caaccgcgtt acctgcactg gaggccagta cctgattcat gtcggtgagc
    38701 agcgcggtgg tgcgcacgcc agcgccgtta gccacgccaa caatcgcttc ggcaagggct
    38761 tcagagagtt cgtaggtcgg cataaacgcg ccgctaccca ctttcacgtc catcaccagc
    38821 gcgtccagac cttccgccag tttcttcgcc aggatcgagg cagtgatcag cgggatggag
    38881 tccacggttg cggtaatatc acgggtcgcg tagaaacgtt tatcagccgg agccagtgaa
    38941 ctggtctgac cgataatcgc cacgccgacg tctttaataa tttcgcggaa acggttgtca
    39001 tccgggaaaa tgtcgaagcc agggatggat tccagtttgt cgagcgtacc gccagtatga
    39061 ccgaggccgc gaccggagat catcggaata tagccgccgc aggctgcgac catcggcccc
    39121 aacatcagcg aagtgacatc gccgacgccg ccggtcgagt gtttatcaac aatcgggcca
    39181 ttcagatgca ggcttttcca gtcgagaacg gttcctgaat ctcgcatcgc catggtcagc
    39241 gagacacgct caggcattgt catatcgtgg aagaaaatgg tcatcgcgag ggcggcaatc
    39301 tgcccttcgg aaatagtgtt gtcgcgaata ccgttgataa agaaacgaat ttcttcatcg
    39361 ctcagcgcat gaccatcacg ttttttacga ataatttctt gtgcgagaaa caaggtaacc
    39421 tcccgaggaa aagagtatga ggcggctttt gccggatgcg tctacgcctt atccggccta
    39481 cgggtagata cgtctcgtag gcctgataag cgcagcgcat caggcgtaaa gcatcttact
    39541 tagtagctgc tggcgctctt accgtcgccg tggcccagcg ctttcagcag gcttgccagc
    39601 aggctggaag caccaaagcg gtagtgacgc gcatcagccc agtcagcacc gaacagttca
    39661 tctgcgatgg cgagatattt ctgcgcatct tccgcagtac gcacgccgcc cgccggtttg
    39721 aaaccaacgg ttttttctac gcccatatca cggatcactt ccatcatgat gcgagcgctt
    39781 tccggcgtcg cgttcacagc cactttaccg gtagaggttt tgatgaagtc cgcacccgct
    39841 ttgatggaga tttcagacgc tttacggatc agcgcttcgt ctttcagttc gccggtttcg
    39901 atgatcactt tcagcagtac attcgctgcc gcgcaagcct ctttacaggc tttcaccagg
    39961 tcaaaaccaa cctgctcgtc acccgccatc agcgcgcggt acgggaacac cacgtcaact
    40021 tcatcagcac cgtaggcgat tgccgcacgg gtttctgcca gcgcgatttc gatgtcgtcg
    40081 ttaccgtgtg ggaagttggt taccgtagcg atacggattt ccggggtgcc ctgctctttc
    40141 agagttttgc gagcaatcgg gataaaacga ggatagatac agatagcggc ggtattgccg
    40201 accggagttt tggcctgatg acacagagca attactttct cgtcggtgtc gtcgtcattc
    40261 agggtggtca ggtccatcaa tttcagtgca cgcaggctgc ttgctttcag atcagtcatt
    40321 tcattctcct ggcttgtcgc caataaaatt caccttgcga gtttgttagt attctaacat
    40381 ctactccgca acacacttcg atacacatca caattaagga aatctactta caagtttgca
    40441 tcactgtaat gcgatctggt tcaaataatt cactttcaaa tgaatgcgtc agtggtggca
    40501 aacgcatcag gatcaaagtg aacatcacga aacttcttac aatggcgcat caccttcggc
    40561 ataaaaggaa aagaagatgc ccacttctca tgaaaatgca ctgcaacaac gttgccagca
    40621 aattgtcacc agcccagtgc ttagcccgga gcagaagcgc cattttctgg cactggaagc
    40681 agaaaacaat ctgccttacc cacagcttcc tgccgaagcc cgccgcgcgc tggatgaagg
    40741 tgtaatctgc gatatgtttg aaggtcatgc gccgtacaaa ccgcgctatg tcttacccga
    40801 ttacgctcgt tttctggcga acggttccga atggctggag ctggaaggcg cgaaagatct
    40861 tgatgacgca ctctctctgc tgaccattct ttaccaccac gtaccgtcgg tcacatcgat
    40921 gccggtctac ctggggcaac tggatgcgtt gttgcaaccg tatgttagaa ttctaacaca
    40981 aaacgagatc gatgttcgaa taaaacgttt ctggcgttac ctcgacagaa ccctgccaga
    41041 tgcctttatg cacgccaata tcggcccgtc tgattcgccc attacccgcg cgatcttacg
    41101 tgcagacgcg caggtttcac cgaacctgac ctttatctac gatcctgaaa tcacccctga
    41161 tgacctgctg ctggaagtgg cgaagaacat ctgtgaatgt agcaaaccgc acatcgccaa
    41221 cggtccggtg catgataaaa ttttcacaaa agggggctac gggattgtga gctgttacaa
    41281 ctcactgccg ctggcgggtg gtggcagcac gctggtacgc cttaacctga aagccattgc
    41341 cgagcgcagc gaatcgctgg atgacttctt tacgcgcact ctaccgcact actgccagca
    41401 gcagatcgcc atcatcgatg cgcggtgtga attcctctat caacaatcac acttctttga
    41461 gaatagcttc ctggtgaaag aagggctgat taaccctgaa cgttttgtgc caatgtttgg
    41521 catgtatggg ctggcggaag cggttaactt gctgtgtgag aaagaaggga ttgccgcgcg
    41581 ctacggtaaa gaagccgccg caaatgaagt gggttatcgc atcagcgcgc aactggcgga
    41641 gtttgtcgcc aatacccccg tgaaatatgg ctggcaaaaa cgcgccatgt tacacgcaca
    41701 gtcggggatc agttccgata tcggcaccac gccgggcgcg cgtttgccgt atggcgatga
    41761 gccagatccg atcacccatc tgcaaactgt cgccccgcat catgcttatt attattccgg
    41821 catcagcgac attctgacgc tcgacgaaac catcaaacgt aacccgcagg cactggtaca
    41881 gctttgcctc ggtgccttta aagccggaat gcgtgaattt accgccaatg tcagcggtaa
    41941 cgatctggtt cgcgttaccg gttatatggt gcgtttgtcg gatttagaaa aatatcgcgc
    42001 cgaaggttca cgcaccaaca ccacctggct gggcgaagaa gccgcacgca acactcgtat
    42061 tctggaacgc cagccgcgcg tgataagcca tgaacagcag atgcgcttta gtcagtaaga
    42121 ttatcccctt ctcctgcgtt gacgggccag gcagtcgtct ggctctgttt ttgcagggct
    42181 gcaatctgcg ctgcaaaaac tgtcacaatc cgtggacgat gggacgttgc aatgactgtg
    42241 gggagtgcgt gccacagtgt ccgcatcagg cgttgcagat tgttgacggc aaagtggtgt
    42301 ggaacgctgt ggtctgcgag caatgtgata cctgcctgaa gatgtgtccg caacatgcca
    42361 caccaatggc gcaatccatg agcgtggacg aagtgcttag ccatgtgcgc aaagcggtgc
    42421 tgtttattga agggataacg gtaagcggcg gcgaagccac gacccaactg ccgtttgtag
    42481 tggcgctgtt tactgctatc aaaaacgatc cgcaactgcg ccatctcacc tgtctggtgg
    42541 acagtaacgg tatgttgagc gaaaccggct gggaaaaatt actcccggtg tgtgacggcg
    42601 caatgctcga tctcaaagca tggggtagcg aatgtcatca acaattaacc ggacgcgata
    42661 atcaacagat taagcgcagc atctatttgc tggcagagcg cggcaagctg gcggaactgc
    42721 gtttgctgat gatcccaggc caggtggatt atttgcaaca catcgaagaa ctggcggcgt
    42781 ttatcaaggg acttggtgat gttccggttc gcctgaacgc gtttcatgcc cacggcgtgt
    42841 atggcgaggc gcaaagctgg gcgagcgcca cgccggaaga cgttgagccg ttggctgatg
    42901 cattaaaggt gcgcggggtg agccggttga tatttccggc gctctatttg tgacatgccg
    42961 gatacggcct acggttcggc acagacttgt aggcctgata agacgcgtca agcgtcgcat
    43021 caggcattgt gcgccaactg ccggatgcgg cgtgaacgcc ttatccggcc tacggttcgg
    43081 cacaaacctg taggcatgat aagacgcgtt aagcgtcgca tcaggcatta tgcgccgact
    43141 gccggatgct gcctacgaag ggcaaactac ccacgaacgt taaacaccgc atacgtgtta
    43201 ttaagcaaca cttcggcaat ctcatccgcg ggttccgggc gtaactcgca aagcacggca
    43261 aacacacggg cagcctgttc cgggcggtta ggctgcccct gaaaaccgtt gagcggcata
    43321 tccggcgcat cggtttccag caacaacgat gccagcggta attttgcgat gacatcgcgg
    43381 gttttactgg cgcgtggata ggtgatagta ccgcctacgc cgattttgta gcccagctgt
    43441 acaaaccgtt cagcctgttg ctggctgcca gaaaatccgt ggacaacacc agcacgcggc
    43501 aaatcgtggc gtttaagatg catcgccagt ttgtcgtgcg tgcgccgtga atgcaggatc
    43561 accggcagat cgtagcgttt cgccagtttc agttgttcgt cgagtaacca ctgctgcctc
    43621 tcaaattgcg gatcgtcgcc gaagagatcc agaccgatct cccccaccgc caccaccttc
    43681 gccggacgcc tttccagcgc ctgctgtagc tgctcaagag acacatcgct atgtttttcc
    43741 aacataccag gatgcaagcc caatacggca tacagcggtt gataattttc cgctaacgcc
    43801 tgcacacggg caaaattttc cgcctctgtc gccggaacaa tgatcttgcc tacgcccgct
    43861 tgtgccgcgc gttgcaggct ggcctcttca tcgccaataa aaggcgggaa atcaaaatgg
    43921 cagtgagtgt cgataaaacg gcaaatcaag ccagatcctc attgttaaat gtcgggtcgt
    43981 tcgcctgcgg agcgtcgctc acttcggcaa ccagcgtatc gttggcgact ggcgcaggcg
    44041 gaatgacaat cgattccggc gtcaccactg gcaccagatg gcgggtaagc ggcgcttttt
    44101 cagttaatag cttgccaacc gtggcgagga aataacgacc gcataaacgc ccaagtttat
    44161 agtcttcacg cagcgccgga atccgactgc caagcgcgat actatgtaat ggcttcggcg
    44221 gataaatttc ggatatccgc agcttgcccg gtggtttctc aataaattgc tgaatgtcac
    44281 gatagctggt ttcatgatgc tgcaccagat tgaccagcgg ctgcaggcta ctgtcaccca
    44341 gccagcgttc catgcgtttg aaccactgcg gcgtgtagta catttgtgac ggcacagtgc
    44401 ggatgacgac caacgtttta gcgccctgcc ttgccgcctc tttaaccgga atcgcatcac
    44461 tgatcccgcc atccaggtag ttaatgcctt ccagcgacac tccgctacga taaaagccag
    44521 gtatcgcact ggaggcgcga atcacatcca gccagttttg tttggttggt aaaaagtaat
    44581 tcggcgcgta gtcatcctga cgacaggcgc acatataaaa cgatttgccg ctgtcaaaca
    44641 accgcgcggc ggtatccatt tgcaacggca tctggcttgc ggtggcctcc accagccagt
    44701 cgagatcgat aagatttcct ccacggacaa agcgcaatgg atcgaaaaat tcgcgttttg
    44761 tggtatagcg catgatgact ttgcgcgcgt aaccgggctg attgcagata aacgccgaga
    44821 ggttctgcgc cccggcagat gtgccgagat aaagatcgaa aggattaaac tgcgcgcgca
    44881 taaactcatc cagcacgcca gccgtgaaaa ttccacgctg tccgccgcct tcgcacacca
    44941 gagctattcg tccaggctgg aacggcctta gcgacaacgg cgcaatatta ccaagcgtta
    45001 caggtattcg ctgccccact tctgccttcc tgtttttgtt gtgaaagaaa gacaaagtaa
    45061 cgcaatttat tattcgctaa aaccgcaaaa gccagtccgc tggactggct ttggataaaa
    45121 ttttcttaat ggtctgaaat ctagggtcgt tttcggccca tgaacaaact cactaggaac
    45181 agaataatcc cgacgacaaa gacaatttta gctgcgcctg cagcggtacc ggccagacca
    45241 ccaaacccaa gtgcggcggc gattaacgcg ataaccagaa atatgatgcc ccaacgaaac
    45301 ataagcgtct cctttaccat agttaatgtc accgcttgat atgagcgtga aaatcactca
    45361 atgcgatatt tttagtgtgg tgcacatcgc gcctcccgac aaagttcggg aggacgaatt
    45421 acttagtttt cagatcattt ttaacgcttt tcacaccatc taccgctttg gcgatacttt
    45481 cagcacggtc actttgtgcc tgagaatcta cggtaccgga gagctgaacc acgccatcgg
    45541 tggtttcaac tttcacttta cgtgaaggga cgatatcgtc cgccagcagt ttggctttga
    45601 tttcactggt ggtggcggtg tcacccgcgt agcccttcac cgagccttct ttagcgtcgc
    45661 gaacgtgcag tttgtcgctg acagaggtca cgccttcaac gcctttcgcc actttcactg
    45721 cctcttcggc ctgggcctga ctttcaacga aaccgctcag ggtcacgact ttttgatcgg
    45781 ttttgacaga gatatcggtg ctcttgatgt tgtcatgatc caccagcgcc gccttcactt
    45841 tcgcggtgat ggcgctgtca tccatgaaat taccgacttt attcatagag ctatcgactt
    45901 tttgccctgc gctttcattg gtagtctgcg cgttgttttc cgcgtaggca gagccggtcg
    45961 cgacggcaga ggtcaacatt acagccagca gagttttcga aatcttcagt cttgtcatag
    46021 tcatcgattt attcctgtat gtttgctcgt aatttgagcc tagcaacacg aggttgcatt
    46081 gctgaatagg gagagacttc accctctaca gaagtcaatg gtcgccatca caaaagcgat
    46141 gggtgatgaa taacgaccat tacagcctct gaatcagtta ttaatatcgg tagaatgaca
    46201 atcgacggct ttagatacta atatctacgc attgaacggt atttaacgcc atcagaaatg
    46261 tcatcacttt gttaaatata gatcacaatt ttgaaaccgc tcgggatatc agcgaaaaca
    46321 taagcaaaag tgaatgtttt aagaacattc cgtaagcggc taataaggaa gggaaattga
    46381 cagggcgcag cggctaccgc gccctggagg caagaattaa tgctcgcggg tctggtggaa
    46441 ctgaacgtcc ggataacgtt cctgtgccag gcgcaggttg accatgctgg tagcgatgta
    46501 agcgaggtta tcgccgccat caagcgccag ttggctttcg ttcttacgct tgaactcttc
    46561 gaatttcttc gcgtctgcac attctaccca gcgggcagtg gcaacgttga ctgactcata
    46621 cactgcttca acgttgtatt cgctcttcag gcgcgctacc accacatcaa actgcagcac
    46681 accaaccgca ccaacgatca gatcgttgtt ggagattgga cggaacacct gcaccgcgcc
    46741 ctcttcggaa agctgtacca gccctttgag cagctgtttt tgcttcagcg gatctttcag
    46801 gcggatacga cggaacagtt ctggtgcgaa gttcggaata ccggtgaact tcatcatctc
    46861 accctgggta aaggtgtcgc cgatctgaat ggtgccgtgg ttgtgcaggc cgaggatatc
    46921 gcccggatac gcttcttcaa cgtgcgaacg gtcacccgcc ataaaggtca gcgcgtcgga
    46981 gatcaccaca tctttcgcag tgcgcacctg gcgcagtttc atgccttttt catatttacc
    47041 ggacaccaca cgcataaacg ccacgcggtc gcggtgtttc ggatccatgt tggcctgaat
    47101 tttaaatacg aagccggtaa atttatcttc gctcgcttct acggtacggg tatcggtctg
    47161 acgcggcatc ggcgcaggcg cccactccac caggccattc agcatatgat caacgccgaa
    47221 gttacccagc gcagtaccga agaataccgg agtgatttcg cccgcaagga acagctcttt
    47281 gtcgaactcg ttagacgcgc ctttaaccag ttccagttcg tcacgcagct gctgtgccag
    47341 atcttcacca accgcagcat cgagatccgg gttattcagc cctttaacaa tgcggacttc
    47401 ctgaatggtg tgccctttac cgctctgata gagataggtt tcatctttat aaaggtggta
    47461 aacgccttta aacagcttgc cgcagccaat tggccaagtg atcggtgcgc agccaatttt
    47521 cagctcgttc tcaacttcat cgagcagttc catcgggtcg cggatatcac ggtcaagttt
    47581 gttcataaag gtgaggatcg gcgtgtcgcg cagacgggta acttccatca gcttacgggt
    47641 acgatcttca acaccttttg cggcgtcgat aaccatcagg cagcagtcca ccgccgtcag
    47701 ggtacgatag gtatcttccg agaagtcttc gtgccccggg gtgtcgagca ggttaaccag
    47761 gcaatcgtga tacggaaact gcatcacaga cgtagtaatg gagatcccac gctgcttttc
    47821 catctccatc cagtccgact tagcgtgctg gttggaacca cggcctttta ctgtaccggc
    47881 ggtctgaatg gcctgtccga acagcagcac cttctcggtg atggtagttt taccggcgtc
    47941 cgggtgagaa ataatggcaa aagtgcggcg cttcgccacc tctttcaaat aaggagacaa
    48001 cgtcataatt ctttcttctt gagtaagcgg catcgcgccg cgcttgttga aaacgaaaaa
    48061 ttgcggctat tttacccatc aacggggggg aggcaatcag tgtttacaca ggagctgctc
    48121 cagttcgtgc aacgaagaaa cggtccaggt gggcgcgatg ccttctggtt gctcgcgatt
    48181 gtgcgcattc agccagcagg tcgcaagccc ggcgttgatg ccaccgagaa tatcggactc
    48241 ggcagtgtcg ccaaccatca gcacgcgtga acggtcagga ttgcccgcct gttccagcgc
    48301 ataatcgaaa attttcttat tcggtttggc aacgccaact tcttcggaaa tcaccagcaa
    48361 atcgaagtaa tcacgcaggc ccgtccgttc cagacgcact tgctgcaagg cgctaaaacc
    48421 gttggtgatg atgccgattt tagcgttgct acgaatggcg ttaagcagag aaaccgcgcc
    48481 cggcaacggc gtacagattt ccgccatcgc attaataaag gcatcgttaa gcaagcctgc
    48541 ttcaactttc agccgttcgg cccagctctc gaaacgcccg tgctgaagct gtaatgaagt
    48601 gatcgcgccg ttttgataat ccacccacag tggcttgtta acggcctgat agtcctgaaa
    48661 atcttcagcg gtaaaggtga cgctgtaatc aagaaacatc cgctgcaggc cggtgaatga
    48721 gtcaaaggta aacagcgttt catcggcatc aaagaaaatc cagtcccact tcattattcc
    48781 accttgtctt acatactgat tggcaacgcc atgatgatgg cgtcttcgcg accgtccgtg
    48841 gtggggtagt aattgcggcg aatcgtcgcc tcgttaaagc ctaaactttc gtacagggca
    48901 atggcggcag catttgaggc acggacttcc agccatagtg tcgccacgcc gcgtttttcc
    48961 agttcgtcga tcagatgttc cagcagcgcc ctacccagcc cctgacgctg atagtcggga
    49021 tcgaccgcaa tattgaacaa tgtagcttca tccagcacca cttgcgtaat cgcaaacgcc
    49081 gccattttgc cgttttgcgt taactgaaag ttgagataac gctcgccctg gttgctggca
    49141 aaggtttttt cactccacgg aaaggcgtgg gcgcgttgtt caatgtggta agccgctggt
    49201 aaatcagtcg tttcgaggga agaaatcgtg ttcatatgtg caaatttgtt gccataacgc
    49261 ggcgcgtgcc gttgggtttg cccgtaattc ggtgagcgcc ggtgatgcca cctgagcgcc
    49321 ttccaatgat agcggttcgt cagtacccaa ccgccagctg ttgcagcgac tgccttgcgg
    49381 cagcatcgcg attttttctg gcgtcagttg cagcacttga tcggggctga cggttaatgc
    49441 gcgcagaaca tcgctcacta aaggatcagt cagggcggga agatcgtttg ccaccatcac
    49501 cagacggacg tgtgccgggc tcgcaatggc aatctccccc tgcaacgcgc cagggcgacg
    49561 cagcgaccac tgggtaatgc ccagttgctg taactgccag tctcgtcggg atgtcatagc
    49621 gaatcgctcc tgttgtcagg ggcgcaaata tagcaaattc gtcgataccg cgccaacata
    49681 tggctataat cgccgccagt atcaattgag gagcattcca tgtctgcatt taccccggca
    49741 agtgaagtct tgctgcgtca cagtgatgat ttcgaacaaa gccgtattct gtttgccgga
    49801 gacttacagg atgacctgcc cgcgcgttta gataccgcgg ccagccgtgc tcatacccag
    49861 caattccacc attggcaggt gttaagctgc cagatggggg ataacgcccg ttttagtctg
    49921 gtcgccacgg cgaatgatgt cgcagattgc gatacgctga tttactactg gccgaagaac
    49981 aaaccggaag cccagttcca gttgatgaat ttactttctc tgctgccagt ggggacagat
    50041 atttttgtcg ttggcgagaa ccgcagcggc gtgcgcagcg ctgagcagat gctggcagat
    50101 tatgcgccgt tgaataaagt cgacagcgct cgtcgctgtg gcctctattt tggtcgtctg
    50161 gaaaaacagc cagtatttga tgcggataaa ttctggggcg aatacagcgt cgatggcctg
    50221 acggtcaaaa cgctgcctgg cgtgtttagc cgcgacggtc tggatgtcgg tagccagttg
    50281 ctgctctcga cgttaactcc gcacacgaaa ggtaaagtgc tggatgtcgg ctgtggcgcg
    50341 ggtgtacttt cagttgcctt tgcgcgccat tcgccgaaaa ttcgtctcac cttgtgcgat
    50401 gtctctgccc cggcggtaga agccagccgc gcaacacttg cagccaacgg tgttgaaggt
    50461 gaagtctttg ccagcaacgt cttttccgag gtgaaaggtt gttttgatat gatcatctcc
    50521 aacccgccgt tccacgatgg gatgcaaacc agcctggatg cggcgcaaac gctgattcgt
    50581 ggcgcggtgc gtcatcttaa tagcggcggc gagctgcgaa ttgtagcgaa cgccttcctg
    50641 ccttacccgg acgtgctgga tgagacattt ggcttccatg aagttatcgc gcaaaccggg
    50701 cgcttcaagg tatatcgcgc cattatgacc cgccaggcga agaaaggttg agtatctgat
    50761 atctgtcgcc gcataccgtg tcggatgcgg cgtgaacgcc ttatccgacc tacaaaacct
    50821 gcacgttagc ccttcgtagg ccagataaga cgcgccagcg tcgcatctgg catctgcccc
    50881 cgcatgccta ccaaacctgc acgctagttt cctgatggac atttttccag caattacaca
    50941 tcaggcgata attaactatt gacgaaaagc tgaaaaccac tagaatgcgc ctccgtggta
    51001 gcaattcttt ttaagaattg atgg
//
LOCUS       DAJTSI010000011        48321 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000011 DAJTSI010000000
VERSION     DAJTSI010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.011"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            187..480
                     /locus_tag="OO327_000574"
     CDS             187..480
                     /locus_tag="OO327_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6017237.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(521..1711)
                     /gene="nepI"
                     /locus_tag="OO327_000575"
     CDS             complement(521..1711)
                     /gene="nepI"
                     /locus_tag="OO327_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCR6017238.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1922..2374)
                     /locus_tag="OO327_000576"
     CDS             complement(1922..2374)
                     /locus_tag="OO327_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR6017239.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="OO327_000577"
                     /gene_synonym="yicO"
     CDS             complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="OO327_000577"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR6017240.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3936..5702
                     /gene="adeD"
                     /locus_tag="OO327_000578"
     CDS             3936..5702
                     /gene="adeD"
                     /locus_tag="OO327_000578"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR6017241.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="OO327_000579"
     CDS             complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="OO327_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR6017242.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="OO327_000580"
     CDS             complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="OO327_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR6017243.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFVSYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="OO327_000581"
     CDS             complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="OO327_000581"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR6017244.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="OO327_000582"
     CDS             complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="OO327_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR6017245.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="OO327_000583"
     CDS             complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="OO327_000583"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6017246.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="OO327_000584"
     CDS             complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="OO327_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR6017247.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="OO327_000585"
     CDS             complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="OO327_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR6017248.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13524..13613
                     /gene="tisB"
                     /locus_tag="OO327_000586"
     CDS             13524..13613
                     /gene="tisB"
                     /locus_tag="OO327_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR6017249.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13893..15077
                     /gene="emrD"
                     /locus_tag="OO327_000587"
     CDS             13893..15077
                     /gene="emrD"
                     /locus_tag="OO327_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR6017250.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(15085..15582)
                     /locus_tag="OO327_000588"
     CDS             complement(15085..15582)
                     /locus_tag="OO327_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR6017251.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15579..15941)
                     /locus_tag="OO327_000589"
     CDS             complement(15579..15941)
                     /locus_tag="OO327_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR6017252.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15931..16278)
                     /locus_tag="OO327_000590"
     CDS             complement(15931..16278)
                     /locus_tag="OO327_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR6017253.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            16387..16800
                     /locus_tag="OO327_000591"
     CDS             16387..16800
                     /locus_tag="OO327_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6017254.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16847..18289)
                     /locus_tag="OO327_000592"
                     /pseudo
     CDS             complement(16847..18289)
                     /locus_tag="OO327_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18324..20039)
                     /locus_tag="OO327_000593"
     CDS             complement(18324..20039)
                     /locus_tag="OO327_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HCR6017255.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20206..21099
                     /locus_tag="OO327_000594"
     CDS             20206..21099
                     /locus_tag="OO327_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR6017256.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDISVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(21096..21910)
                     /locus_tag="OO327_000595"
                     /pseudo
     CDS             complement(21096..21910)
                     /locus_tag="OO327_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21910..23526)
                     /locus_tag="OO327_000596"
     CDS             complement(21910..23526)
                     /locus_tag="OO327_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR6017257.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            23821..24495
                     /locus_tag="OO327_000597"
     CDS             23821..24495
                     /locus_tag="OO327_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6017258.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24534..26195)
                     /locus_tag="OO327_000598"
     CDS             complement(24534..26195)
                     /locus_tag="OO327_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR6017259.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26391..26819)
                     /gene="ibpB"
                     /locus_tag="OO327_000599"
     CDS             complement(26391..26819)
                     /gene="ibpB"
                     /locus_tag="OO327_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR6017260.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26931..27344)
                     /gene="ibpA"
                     /locus_tag="OO327_000600"
     CDS             complement(26931..27344)
                     /gene="ibpA"
                     /locus_tag="OO327_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR6017261.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27338..27410)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27649..27981
                     /locus_tag="OO327_000601"
     CDS             27649..27981
                     /locus_tag="OO327_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR6017262.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27983..29215)
                     /locus_tag="OO327_000602"
     CDS             complement(27983..29215)
                     /locus_tag="OO327_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR6017263.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTWTPDSQWLVFDVRPSGASFT
                     GETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHR
                     RGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPA
                     LDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVG
                     NHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQR
                     RLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTH
                     NKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDG
                     QWLAWMEGGQLWITETDR"
     gene            29316..30416
                     /gene="cbrA"
                     /locus_tag="OO327_000603"
     CDS             29316..30416
                     /gene="cbrA"
                     /locus_tag="OO327_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCR6017264.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30377..31714)
                     /locus_tag="OO327_000604"
     CDS             complement(30377..31714)
                     /locus_tag="OO327_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6017265.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31789..32937)
                     /gene="dgoD"
                     /locus_tag="OO327_000605"
                     /pseudo
     CDS             complement(31789..32937)
                     /gene="dgoD"
                     /locus_tag="OO327_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32934..33551)
                     /gene="dgoA"
                     /locus_tag="OO327_000606"
     CDS             complement(32934..33551)
                     /gene="dgoA"
                     /locus_tag="OO327_000606"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCR6017266.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33535..34413)
                     /gene="dgoK"
                     /locus_tag="OO327_000607"
     CDS             complement(33535..34413)
                     /gene="dgoK"
                     /locus_tag="OO327_000607"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCR6017267.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34410..35099)
                     /gene="dgoR"
                     /locus_tag="OO327_000608"
     CDS             complement(34410..35099)
                     /gene="dgoR"
                     /locus_tag="OO327_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCR6017268.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLAAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35377..36033
                     /locus_tag="OO327_000609"
     CDS             35377..36033
                     /locus_tag="OO327_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6017269.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(36079..36891)
                     /gene="yidA"
                     /locus_tag="OO327_000610"
     CDS             complement(36079..36891)
                     /gene="yidA"
                     /locus_tag="OO327_000610"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR6017270.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(37005..37403)
                     /locus_tag="OO327_000611"
     CDS             complement(37005..37403)
                     /locus_tag="OO327_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR6017271.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37643..40057)
                     /gene="gyrB"
                     /locus_tag="OO327_000612"
     CDS             complement(37643..40057)
                     /gene="gyrB"
                     /locus_tag="OO327_000612"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR6017272.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(40086..41159)
                     /gene="recF"
                     /locus_tag="OO327_000613"
     CDS             complement(40086..41159)
                     /gene="recF"
                     /locus_tag="OO327_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR6017273.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(41159..42259)
                     /gene="dnaN"
                     /locus_tag="OO327_000614"
     CDS             complement(41159..42259)
                     /gene="dnaN"
                     /locus_tag="OO327_000614"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR6017274.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(42264..43667)
                     /gene="dnaA"
                     /locus_tag="OO327_000615"
     CDS             complement(42264..43667)
                     /gene="dnaA"
                     /locus_tag="OO327_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR6017275.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43964..44044
                     /locus_tag="OO327_000616"
     CDS             43964..44044
                     /locus_tag="OO327_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6017276.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            44274..44414
                     /gene="rpmH"
                     /locus_tag="OO327_000617"
     CDS             44274..44414
                     /gene="rpmH"
                     /locus_tag="OO327_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR6017277.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44431..44790
                     /gene="rnpA"
                     /locus_tag="OO327_000618"
     CDS             44431..44790
                     /gene="rnpA"
                     /locus_tag="OO327_000618"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR6017278.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44754..45011
                     /gene="yidD"
                     /locus_tag="OO327_000619"
     CDS             44754..45011
                     /gene="yidD"
                     /locus_tag="OO327_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR6017279.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            45014..46660
                     /gene="yidC"
                     /locus_tag="OO327_000620"
     CDS             45014..46660
                     /gene="yidC"
                     /locus_tag="OO327_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR6017280.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46766..48130
                     /gene="mnmE"
                     /locus_tag="OO327_000621"
     CDS             46766..48130
                     /gene="mnmE"
                     /locus_tag="OO327_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR6017281.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11982 a        13064 c        12218 g        11057 t
ORIGIN      
        1 gaggtaaggt cgctgcggtt taaaaaacga aacgctatcg ataagaataa aaaggaataa
       61 aagtggaata taaagtctgg cacttcttac tgaccacgca agcgcgtttc gtacagcatg
      121 atgagagcga tgaaagcaaa ctacacttat gcttcattcg atacacattt gtaaaaggtt
      181 gaatccatga aaccaacgac actactgctt attttcactt ttttcgctat gccaggcatc
      241 gtatacgccg agtcgccttt tagctcactg cagtccgcaa aagagaaaac cacggtatta
      301 caagatttac gtaaaatttg cacgccacat gcgtcattat cagatgaagc gtgggaaaag
      361 ttaatgttgt cagatgaaaa caataaacaa catatacgcg aggccatcgt ggcgatggaa
      421 cgtaataatc agagcaatta ttgggaagcg ttgggtaagg tagaatgccc ggatatgtaa
      481 gaacagcaac aactactgac caacgcttct ttacctgata tcaggatttc ttcattttca
      541 cctttgcagt aaccaacaat gcagtcagca acatcaatgt gccggacaac atcaacggcg
      601 aagtcagacc aatattatcc agcgcataac cgccgattgc cgcgccacag gtattagcaa
      661 gctgaataac cgccacctga atagacccgg ctttttctgc ctgatcggcc agcgagcggg
      721 tgatccacgt tgaccagccg acgggaacca atgcaaaagt tagcccccag ataatcgcca
      781 cgccggtagc aacgatttta tcgcttcccc acaacgtcag taccaacgca ctcacagcca
      841 gtattaacgg cgcgcctgct aaggccagtt ttaccgaacg tttaagaatg aacgacgaaa
      901 gcgacgtacc aataaagctg gcgataccaa aactcaacag caccagcgtt aagccatcca
      961 cgccgaatcc cgccaggttc atatacaccg ggcgaatata cgtgaagaaa gcaaactgcc
     1021 cggcgaaaga catgaagatg gcgatcatcc ctgccatcac acccggacgt tgtaataagc
     1081 ggaaagtatt ttgtttctga tgcgagggtt cgcctggcag tgaaggcaat gatttgataa
     1141 tccagaaaat acacagcacg cccatcaccg ccgccgcatt aaagacattg cgccaaccga
     1201 taagctcgcc taaaaaactg cccaacggcg cggcaatcac cagcgcaata gaaaccgcgc
     1261 cgaagatcac cgacagcgcc ttcggcaccg tacgcggcgg taccagacgc atggtcagcg
     1321 acgccgacat cgcccagaac ccgcccagcg ccagccccag acaggcacga ccgattaaaa
     1381 gcaaactgaa tgagttagca aaggaaacca gcaagcagga gagcgtcagc aaaacggcaa
     1441 acaaaataac aacgtagcgg cggtcagtgg cctgaattgt ctgggtaata aacaaactgg
     1501 caaacattgc cacaaaggcg gtcacggtca ccgattgccc ggcaacccct tccgaaatgc
     1561 ctaaatcctg ggccattggc gtcaacaaac tgacgggcaa aaactcaacg ataatcagac
     1621 aggcgacaca aaacgccacc gagaaaacgg ctgaccagtt cggtcgggtg atggcatccg
     1681 cgccgcggtt ttcggcaata aattcactca tggtgttacc cgtggcatgt ttcaggggaa
     1741 aagccgacca ctttaacatt gaaatgtgtg acgaatttaa cgtttttgca acttttacgg
     1801 tgttgcttga gctggagtta agatcgaaac ggaacagggc aaagattacc ggatgcggcg
     1861 tgaacgtctt atccacccga caggtcaggc gcggtctgta ggctgataag acgaaacggt
     1921 ttcaatgcaa ccagtgaacg ccctcggcag gctgaaagaa cgcgttatag cgcatctgaa
     1981 aagcattaat atcctgcatt tcaggttcgg tttcacgcag aaaacccagc gccagcctga
     2041 cctgagcatc ggtaatcggc gcggaaaggc gggctttttg taaaatccgg tgccattgca
     2101 gcaggttctg ttcgctatca tcgacaatga caatttgcca gataagcaac acctgcgcgg
     2161 cgttttgcag gtgcgactca tccgggcgat gcaaataacg taaaaattcg tcaccggctg
     2221 actttcccat ttgatcgacc atcgactcca cgggtacgac atcgatattc agccgatcgc
     2281 tgagacggcg aatcgctttt ctggccccgg atgtcagcac ccgaaaacca accacaaaca
     2341 ctaccgccag cgtggcgagc attatccaga tcatgagttc tcctgcgtaa tcagaactca
     2401 tgatatctgg aaaccctcgc cagttactaa tccaccagaa taatcttcaa tgcgaacaga
     2461 gctgcgacca ccaccacaca caggttcaga tcgcgccagc gcccggtgca cactttcatg
     2521 atgcagtacg acataaagcc gagtgcaatc ccttcggtga tcgagaaagt aaagggcatc
     2581 atcaccgtgg taataaacgc aggcaccgat tcggtaaaat catcccagtt aacgcgcgcc
     2641 aggctcgaag tcatcagcac gccaacaaag attaacgctc cagcggttgc gtaaggagga
     2701 accatcgcca ccagcggtga gaagaacata actaacagga acataacgcc gaccacgaca
     2761 gccgttagcc ccgtgcgacc accgactgcc acaccagaag tactttcaat ataggcagta
     2821 acagacgagg taccgataaa tgcgcctgcc accgaactga cgctatcaac atacagcgcc
     2881 ttattcatat tggggaattt accgttgcta tctattaagc ccgctttatc agttacacca
     2941 attaatgttc ctgatgaatc aaataggttg atcagcataa aggagaaaat gataccggcg
     3001 agttcaagtg ttaacgcgcc gctcaaatct acttcaccaa tgacgccgct aatatcaggc
     3061 ggaatggaat agacgccgct aaaatgaaca tcaccgaaaa ataatccaca gcaagacgtc
     3121 accacaatag aaaccagcac cgcggcatga aaatgacgtg atgacaacac ggttataata
     3181 aaaaacccta aaatacctaa caacacgccg tgagaactta aatcgccaat catcaccagc
     3241 gtgtctttat tggcgacaat aacaccagtg ttttttaatc ccattaaggc aataaataat
     3301 ccgattccac tggtgatacc aatacgtaaa cttaatggaa tgttggagat catccagtac
     3361 cggatacgaa acagcgtgag caaaaatagc ccaattgcgc cccagaatat cgcgcccatc
     3421 ccggtctgcc aggagatccc catcgccccc acgaccacga acgcaaagaa ggcgttcagc
     3481 cccattgccg gagccagcgc cacgggtaag ttagcaaata tccccatcgc aatactgccg
     3541 ataccggcaa tcaaacaggt ggtaacaaac accactttcg ggtccatttg tgccgcgccg
     3601 aggatttgcg ggttcacaaa aacaatgtac accatggtta aaaaagtggt catccccgca
     3661 atcaattctg tgcggacggt ggtcccatgc tgaggtagtt taaataatcg cgaaagcgtc
     3721 cctgattcat tactcacgta atcggtattg tcattattca tttttttgtc cattggagga
     3781 gatttaatcc cttgcttttg taaaacaaat gacaacaacg gttcagtgat aattatcaca
     3841 tttcaattgc acattaatag atattcttta ataatctcgc gacgtttctt tatgataaat
     3901 aataatcaaa ttgataaaat caaaatgaga aaaatatgaa taattccatt aaccataaat
     3961 ttcatcacat tagccgggct gaataccagg aattgttagc cgtttcccgt ggcgacgctg
     4021 ttgccgatta tattattgat aatgtctcta ttctcgacct gatcaatgcc ggagaaattt
     4081 ccggcccaat tgtgattaaa ggacgttaca ttgccggtgt tggcgcagaa tacgctgata
     4141 ctccggcttt gcagcggatt gatgctcgcg gcgcaacggc ggtgccaggg tttattgatg
     4201 ctcacctgca tattgaatcc agcatgatga cgccggtcac ttttgaaacc gctaccctgc
     4261 cgcgcggcct gacgaccgtt atttgcgacc ctcatgaaat cgtcaacgtg atgggagaag
     4321 ccggattcgc ctggtttgcc cgctgtgccg aacaggcaag gcaaaaccag tacttacagg
     4381 tcagctcttg cgtgcccgct ctggaaggct gcgatgttaa cggtgccagt tttacccttg
     4441 aacagatgct cgcctggcgg gaccatccgc aggttaccgg ccttgcagaa atgatggact
     4501 accctggcgt aattaacggg cagaatgcgc tgctcgataa actcgatgca tttcgctacc
     4561 tgacgctgga cggtcactgt ccgggtttgg gtggcaaaga acttaacgcc tatattgctg
     4621 cgggtattga aaactgccac gaaagttatc agctggaaga aggacgccgg aaattacaac
     4681 tcggcatgtc gttgatgatc cgcgaagggt ccgctgccag caatctcaac gcactggcac
     4741 cgttgatcaa cgaatttaac agcccgcaat gcatgctctg taccgatgac cgtaacccgt
     4801 gggagatcgc ccatgaaggg catatcgatg ccttaattcg ccgcctgatc gaacaacaca
     4861 atgtgccgct gcatgtggca tatcgcgtcg ccagctggtc gacggcgcgc cactttggtc
     4921 tgaatcacct cggcttactg gcacccggca agcaggccga tatcgtcctg ttgagcgatg
     4981 cgcgtaaggt cacggtgcag caggtactgg tgaaaggcga accgattgat gcgcaaacct
     5041 tacaggcgga agagtcggcg agactggcgc tgtctgcccc gccatatggc aataccattg
     5101 cccgccagcc agtttccgcc agcgactttg ccctgcaatt cacgcccgga aaacgctatc
     5161 gggtcattga cgtcatccat aacgaattga ttacgcactc ccgctccagc gtctacagcg
     5221 aaaatggttt tgatcgcgat gatgtgtgct ttattgccgt acttgagcgt tacgggcaac
     5281 ggctggctcc ggcctgtggt ttgctcggcg gctttggcct gaatgaaggc gcgctggctg
     5341 cgacagtcag ccatgacagc cataatattg tggtgatcgg tcgcagtgcc gaagagatgg
     5401 cgctggcggt caatcaggtg attcaggatg gcggcgggct gtgcgtggta cgtaacggcc
     5461 aagtacaaag tcatctgccg ttacccattg ccgggctgat gagcaccgac acggcgcagt
     5521 cgctggcgga acaaattgac gccttgaaag ccgccgcccg tgaatgcggt ccgttacccg
     5581 atgagccgtt tattcagatg gcgtttcttt ctctgccagt gatccccgcg ctaaaactaa
     5641 ccagtcaggg gctatttgat ggcgagaagt ttgccttcac tacgctggaa gtcacggaat
     5701 aataaaaaaa gcccggcgtc atgccgggca aaagtcacca gttacgttta tgccactgtc
     5761 aactgctgaa tttttttctc gcggcggatt ttgcgttctt ccattaccgc cactatcgcc
     5821 atcaggcaga tacaaccaat cgcggcgata tccagcgcgg cgaaggtgcc tgcccagccg
     5881 gtaaggccga ataccggcgt cccatcggca atcattccca gacctaactt ggcaaagctg
     5941 tcaccaatca ggtaagcaaa ggtgccttta ataccatcgg cagcgccaat cgctttttta
     6001 ggtacaaagc caacagcagc cacaccaatc aacaattgcg ggccaaagac caggaaaccc
     6061 aacgcaaaga gagaagccag atagatatat tcgttactgg catgttgata cacaccgagc
     6121 gtggcgataa tcagcgccag cgcgatgcag gccaccaggc cacggcgacc gttcgccagg
     6181 tcagagagcc agccccacag cagcgtaccg accagcgcac cagcttcaaa cagcgtaaag
     6241 ccctgaatcg ccaccgcttt agagagtttc agttcctgga acgcgtatac ggttgaccac
     6301 tggtcgatac caatacgtac cacatagagg aaaatgttgg cgaagcacag cagccagatc
     6361 actttgtttt tcagcacata ctcaacaaag atctgccact tggtcatatc ggtagattct
     6421 gtctctttgt cctcttcgct gatctcctcg ccgaacagtt cttcagcttt gccgaggcca
     6481 taagattccg gggagtcgct gccgtaacgc aggccgataa aaccgacaat cagcgcgata
     6541 atcgacggga agataaacat gccgatgaca tggccatcga acaggtaatt tgccccgaac
     6601 agcgccacac ctgctgcgcc tgcaccgcca aggttgtgag aaatattcca gaaaccgagg
     6661 aatgtcccgc gtttacgacg cggcgtccat ttggtgatgg tggagtaact gcacgaaccg
     6721 ccggtactct ggaaaaagcc gcttaaggcg tagaaggcaa tcatcaggaa caggctaacc
     6781 gagccgctgc ccatactggc actgaagccc agcatacaaa tggcagagag gataagcatg
     6841 aacggcagga attgtttggt gtttttgccg tcggcgtagt atgaaaccaa cgttttaccc
     6901 acgccataag tgatggagaa acccaggccg atcatcccca gctgcgtcat gctcaacccg
     6961 taggtcgaaa tcatatcgtt ctgcgcgatg ttaaagttct tgcgaatcag gtacatcgtc
     7021 aggtagccga taaagaccac caggtaggat tgcatgaacg gtttgaacca cattttgcgc
     7081 cgcacttcga gcggaaggtc cagggtcggc ttgcgaactt ggtttaggaa agccagcatg
     7141 ggttactcct gaaatgaata cctgcgtgag gcatgcattg taaaaatcag cagcaaatca
     7201 ggcctgagat agcgtccaag aaaaacctgg aaaatttctt agttttgccg gatatgaagt
     7261 gaaaaggtga gatgcatcac gcttcacgcg gtgtctgggc gttcaaaaag ggcagtaaca
     7321 gcagtgcgga aatcccggcg gcgatagaga taaccacaaa aaatccgctc cagtgccagg
     7381 tatcgagcac tttcgccagc ggccaaccag caagcgacgc ccccagataa gcaaacaagc
     7441 cgacaaaccc cgtcgccgcc cctgccgcct ctttgtggga acactctgcc gccgccatac
     7501 caattaacat ctgcgggcca aagacaaaaa aaccaatggt gaagaagcag gttgcctgca
     7561 tcacgtagct gacaaatggc atcagccaca gcgagccgac tgaaagcaaa attccggcgg
     7621 cgaaaatcaa attcatcggc ccacggttgc cgttaaacaa tttgtccgag ccccaaccgg
     7681 ctaccagcgc accgataaat ccgcccagtt caaacatcgt cactgccgta ttcgccgtga
     7741 ccagatcgac gcccagcgtc tcggacatat acaaattgcc ccagtcgttg atcgccgccc
     7801 ggaccacata gaccagcaca tagcaaaacg aaagcagcca gatatacgga ttcagcaaca
     7861 catatttggt gaggatctct ttacgcgtca accctgcccc ttcttgttgt tgagcaattt
     7921 ccagcgcgtc gtgccgccat tcaccgaccg ccggtaaacc taacgcctgc gggcgatcgc
     7981 gtagccgcca gcagagaaaa atccccacga ctatcgccat acaaccagca atcatcatcc
     8041 cggcacgcca gccgtaatgc agcgcgctcg ctgccatcac aatgggaatg agtgcgccgc
     8101 cgacgttatg cgccgtgttc cataatgccc accaaccgcc gcgctcggta cgtgaatacc
     8161 aggccgttaa cagacgcgca cacaccggtg aaccccagcc ctggaaaaag gcgttcagca
     8221 cccagagcac ggcaaacgcc catagcgacg tcgagaagcc aaacagaatg ttcatgatgc
     8281 ccgtggcgat aagccctatc cccataaaat aacgggcatt tgagcgatcg ctgacaatgc
     8341 cggagacaaa cttcgacacg ccgtaagtaa tgtaaaacag ggtcgctaac aggccgatat
     8401 cgctacggct gagcacgccg ttagcaagga tttctggtac cgcggcgtta aaacttttcc
     8461 gcgtgaagta aaacagcgcg taacccagcc agatggtcag cagaatatgc cgacgccagt
     8521 agcgataacg ggcatcaatt tcatgtttat cagtcattaa tggcgcatcg gcaggcgctt
     8581 tcagaaacgg caacatcatc gaaccttaga cataacgttg aggtagagaa acgctgacac
     8641 gcgtgccgtg cagacaggaa atgtgtaatg tgccgcccag cgccgttacg cgctcgcgca
     8701 ttccggtgag gccaaaacct tgttgcccgg aacccggcgg caaaccgctg ccatcgtctt
     8761 caatcaccag catcaaccgt tcatcctgct gccagccttg cagcgtaacc gcgctggcat
     8821 cggcatgttt cacaatgttg ttcagccctt cctggcagac acgaaacagc gtcacgcgct
     8881 ggttttcgct taatgctgat tcatcgatac gccattcgag atggctgaca atgccgcgcc
     8941 cttccagctc catttcccgc atcagtgagc ggatggcctg ctccagggtg agatcatcca
     9001 gctggcgcgg acgtaaccgt cctaacaaac ggcgcaccgc gtcgtaaacg cccagcgaca
     9061 gttgttcgat gagctgcccg ctctgcttca cactggcgtt atctgccgcc agccgctgaa
     9121 caatgcccgc ctgagtacga atagcagtga tggtctgacc gatatcgtcg tgcagctcac
     9181 gcgccacgtc gcggcgcacg ctctcttcgg tttctaacaa acgttcagcc agatgctgat
     9241 tgcgcgccag ttccttttgt agcgactggt taagttcacg caaccgctgg atgcctgcgc
     9301 ccagtaacaa ccctgtcaga ctttgcacca gcaacgagag taataaatcc accagatgat
     9361 cgcgccaggt ttgactggcg atcagcgcga tggcgttcat caacgtcgca atcagcgctc
     9421 cttgccagcc atagtgccag gccagcgcga taatcggcag cgccagacag aatggcgtaa
     9481 agcgcgacag ttcgtccggc aatcccaact ggagccagag actgataaca aacagcagca
     9541 agtaccagac cagatgccgc ccgcgccagt tgatgggctg agaaaccagt gacgggccga
     9601 gcggcagcca ggtgttattg gcgagatagt gccagaacac cagacatatc ggggccagcg
     9661 tcagaccgcc agttaaagtc agcaacagcg cattccacga ctctttgccg tgccaaagcc
     9721 agggcagcga ctgcaacaac gccgccgccg ttaacgccgc accctgtaac agcaaggtgc
     9781 gccagtcacg ctgatggcga tagcgcgaga tcagcgctac gggcagtaac gtcagcaaac
     9841 taccgatcat caataacgga aaatgggtta aaccgaccgc ctgcgttagc cagtaaatca
     9901 gcagccactc cgcacccagc aatacgggcc agtatccgcg cgggcattgc agcattagcc
     9961 ccagacgcag accaaacgga aataacagca ccgccatatc agggcgctca accagatgca
    10021 ggctgatact ccataggcaa aaccatgcgg cagagaagat aaaaaagcag gcaataacgg
    10081 taattaagcg ggagaacaac gtcttcatca ccagccatca aacatgcggc gcgccagctc
    10141 tacgtcgtta ctgacgccca gtttttccat cagattggcg cgatggacgt gtaccgtttt
    10201 cggtgacaag cccagttcgg cggcaatctc tttcaccgcc attccttgcg ccagtttttc
    10261 cgccacctgg cgttcacgtt tggtcagcgg gtcctgacga ccggatgcca gtttaatggc
    10321 aatatccggc gtcagataac agccgcccgt ggcaaccgta tgcaccgcag caatgagttc
    10381 atccgggcta cagcgtttgg aaagaaagcc gcgtgccccc gcgttaagcg cctgctcaac
    10441 cagcgcagga ctgtcgtgaa cggagagcat aatcgtcgcc atacctttcg gcagctggct
    10501 tagcagctcc agaccggaga tatcgggcat ggagatatcg caaatacaca cctgcacacc
    10561 gcgccccggc agccccgcca gcgcctcgcg ccccgaacca aactcggcaa ctacctgcaa
    10621 atcaggttcc agccccagca gctgcgcaaa gccggagcgg acgatgaggt gatcgtctat
    10681 aagggcaacg gtgatcatgg tcttgtcctg gcgggtaaaa aaaaacgcgc ttaccttaac
    10741 gataagcgcg atgttgttca agccttgagc ggttactgaa aaaacaccgc gatcttgtta
    10801 aacatcgtcg gatcggactg attacgctgc actttcacga catcttccag cttagcgatt
    10861 tggcttatca tctgctccag acgctggtcg tcattgacca gtagccagat atggcttttg
    10921 tcgctgtcct gaatcggcag acaaagaatg ccttcaacgt taaaagcgcg gcgggcaaaa
    10981 aggccacaaa cgtgggtcat tacgcccgga tggttgcgaa cggtgagctc cagaattacg
    11041 ttgttatgag ttgtgttttg catggcttat tcccccacca tttcagtatt cgccgcacct
    11101 ggcggcacca tcgggtaaac tttttcttcg gcatcaatgc gcacatggat cagcgccggg
    11161 ccagggcgat tgatgatttc ctgcaatgca gcctgcggat cggcttcgtt attcaaatca
    11221 caggtttcga ggccgaatcc ggcggcaatc tgcataaagt tgattttgcc cggataggtg
    11281 gcggcaaaaa cgccttgctc gtagaacaga ctctgttgct gatgcaccag ccccagcgct
    11341 tcgttgttca tcagaatgat tttgacatcc agctgatttt cactggcggt cgccatctcc
    11401 tgaatattca tcatcaggct gccgtcgccg gagaaacaca acactttgcg atccgggttc
    11461 gccagcgccg cgccaatcgc cgcaggcagg ccaaaaccca tcgtgcccag cccaccggag
    11521 gtcagccact ggcgtgggcg attgagcgga taagcttgcg cggtccacat ctgatgctga
    11581 cccacatcgg tggtgataat cgcattgtca tcgacacagg cggcaacggc gttgatcagg
    11641 ccgtaatggc ttaatggatc gcacgctttc gggattggac acgggaattc gcgctgcaaa
    11701 tccgctacca actggtgcca ctctgcacgc ggttgcgctt ccacccgcgg gatcaattgc
    11761 gccagcacgt catcaacatc cgcctgaatc gccacatgcg gctgcttgat tttacccagc
    11821 tctgcgcgat cgatatcgac atgaataatt ttggcattcg gacagaactg ctcggttttg
    11881 ccaatcgccc ggtcatcaaa acgcgcaccg agcacaatca acaaatccgc ctcctgcaaa
    11941 atatagttgg tactgcgcac gccgtgcatc cccagcatac ccagcgacaa cggatgcgct
    12001 tttggcagca tgcccagcgc cattaaagtc atggtggtag gcagttgcgc tttctccgcc
    12061 agttcacgca cccgcgcagg cgcattgatc acaccaccac ccagataaag caccgggcgt
    12121 ttggcagcgt taatcattgt agctgcgtca cgaatgcttt cttcgctaaa ggcgggtgca
    12181 acggcttttt ctgccaccgc gggctgagct tcaatctcaa aaaccgccgt ttgtacatcc
    12241 ttaggaatgt ctatccacac cgggctaggg cggcctgatt gcgcaatgcg gaaggcatcg
    12301 ctcatgacct gtgggagctc ttcgatatgt ctgaccagat agttgtgttt ggtgatgggg
    12361 atagagatgc cgtaggtgtc cacttcttgg aaggcgtcgg tgccgatcat cgaggcggga
    12421 acctgaccag tgatgcaaat cagcgggatg gagtccagcc gcgcatcagc aatggcggtc
    12481 accaggttag tcgcacccgg cccgctacag gccatacaga ccgccggttt accgtcggtg
    12541 cgcgccattc cctgagcgat aaaccccgcg ccctgttcat ggcgagccag aatatggcgg
    12601 atttgcgtgc tttggcttaa ggcgtcgtaa acaggcagga tagaaccgcc cggaatgccc
    12661 gtcacaatct taatgccctg ctgttccagg aaatgaacga taaattctgc gccggtaaag
    12721 cgcttacgcg tcgatgttgt gcccgaactt gccatgctcc agtccttttc ttctgggccg
    12781 actttccggg aggtgcttaa acgaaaaacc ccgcccggtt tgcgccggcg gggttttgga
    12841 atcgtgtgtt gttccagtcc ctacggcgca ttgccgacga ccaccaccac acgcacgacg
    12901 accactgcgg cggatggcgc agttggtagt agttttgcgt tgaacatgga agtagtcatt
    12961 ggggaccttg ttggttttgt gtttaacaat atttatacaa gcacagcttt acaggggaga
    13021 caatggaaaa tttttcagca agggaaaatt gaggggttga tcacgttttg tactgaattg
    13081 cagataacaa aaaaccccgc cggagcgagg tttcgtcagc cgcctgcggc tggtaaccgc
    13141 aaagcacact gtattatgtc aacactgaaa gtatacgtgt tccgcgcaga acgcgcaatt
    13201 tcggcacgaa ttttgacgta tttagtgcat agttgagtat cgttcacagt ttgcgttttg
    13261 tccaaatatt actgtttatt tatacagtaa gcttctataa tatcactgta cgcaatgtgt
    13321 tatgcggggg ccgcatcgtt acccggcgca ctaagtcctg gctgaaacgg gtggtgccgt
    13381 cagcgcctta accccgcgtg agcacactgt gttatgtcaa caagcacaac gtttctcctt
    13441 gagataccgc gtgcacaaca gctggcaaca ggcagcggaa aggtacgtca gctggcagtg
    13501 ctcctgaacc acaggagacg cgtatgagcc tggtggatat cgccattctt atcctcaaac
    13561 tcattgttgc agcactgcaa ctgcttgatg ctgttctgaa atacctgaag taattcagat
    13621 tcaagtcgca ccaaagggga gcgggtaacc gctccccttt tatatttagc gtgcgggttg
    13681 gagtcggatg cgatgctgac gcatcttatc cgccctacca tccctcccgg caacatttat
    13741 tgccgctttt gtttacatat tctgccgcta aacaattccc cattcctggc gtatatctgg
    13801 ctaacattca tcaatgtgat agattcctct cccgcattta tgggaatgcg tagtgactta
    13861 ttctaattat ttttataaaa gcatccgtga taatgaaaag gcaaagaaac gtcaatttgt
    13921 tattgatgtt ggtattactc gtggccgtcg gtcagatggc gcaaaccatt tatattccag
    13981 ctattgccga tatggcgcgc gatctcaacg tccgtgaagg ggcggtgcag agcgtaatgg
    14041 gcgcttatct gctgacttac ggtgtctcac agctgtttta tggcccgata tccgaccgtg
    14101 tcggtcgccg accagtgatc ctcgtcggaa tgtccatttt tatgctggca acgctggtcg
    14161 cggtcacgac ctccagtttg acggtattga ttgccgccag cgcgatgcag gggatgggca
    14221 ccggcgttgg cggcgtaatg gcgcgtactt tgccgcgtga tttatatgaa cggacacagt
    14281 tgcgccatgc taacagcctg ttaaacatgg ggattctcgt cagtccgttg ctcgcaccgc
    14341 taatcggcgg tctgctggat acgatgtgga actggcgcgc ctgttatctc tttttgttgg
    14401 ttctttgtgc tggtgtgacc ttcagtatgg cccgctggat gccggaaacg cgtccggtcg
    14461 atgcaccgcg cacgcgcctg cttaccagtt ataaaacgct tttcggtaac agcggtttta
    14521 actgttattt gctgatgctg attggcggtc tggccgggat tgctgccttt gaagcctgct
    14581 ccggcgtgct gatgggcgcg gtgttagggc tgagcagtat gacggtcagt attttgttta
    14641 ttctgccgat tccggcggca ttttttgggg catggtttgc cgggcgtcct aataaacgct
    14701 tctcaacgtt gatgtggcag tcggttatct gctgcctgct ggctggcttg ctgatgtgga
    14761 tccccgactg gtttggcgtg atgaatgtct ggacgctgct cgttcccgcc gcgctgttct
    14821 ttttcggtgc cgggatgctg tttccgctgg cgaccagcgg cgcgatggag ccgttcccct
    14881 tcctggcggg cacggctggt gcgctggtcg gcggtctgca aaacattggt tccggcgtgc
    14941 tggcgtcgct ctctgcgatg ttgccgcaaa ccggtcaggg cagcctgggg ttgttgatga
    15001 ccttaatggg attgttgatc gtgctgtgct ggctgccgct ggcgacgcgg atgtcgcatc
    15061 aggggcagcc cgtttaagcg cacgtcaccg cagcatcgtc atcagctcca tgggagaacg
    15121 atgctgcttt atcagatcac gcatcacccg catatgcggt gcggagtaag aataaaacgc
    15181 ctgatagccc gcacaaagca cgctgtctgg cgtctcctgc cgagcgtgcg ggcacaggcg
    15241 atgccagtcg cacgcctggc agtcacggcg cggttgcggg ttttcgctgc cacaccattt
    15301 ttgtaaggtg gcgtcaaaaa gctcaatcga gatacgactg atatctgccc gcacccaaca
    15361 ctcaaacagg gcaattaaaa atttccccca cagctctgct gtcaccgaag cggccgtcag
    15421 atcgccccgg tcatcaaatt caagataggg aatgagctgt acggagcgga tgccccgctg
    15481 tttgaaattg tgataaaaat cggcaggttg cagagcatcc ttgtactcta caaccagctt
    15541 atgcctgtcc tcttcagcat caatgacctg acttcctgtc atgcgactct ctcaataaat
    15601 acgataagtt gatgtatatg cgttacagca aacagtattg cgagagataa caccgcgagg
    15661 gagatcaaaa atttgtcacg gactacgtga aattgggaaa aatcgctatt cgtgacatcc
    15721 attaaattgc gattacgggt gtagtgccag aggatcagcg ccacgatggc gaggatgcca
    15781 atcgaaatcc agaataacat gcccgcctgg tgccagttgt gtttgatagc caacgccatc
    15841 aacgcgccgt agcccagcat ggtacgaaac cacgccagtg atgtgcgctc cggctgcagc
    15901 cctgggtcgg caatgcgtct ggctttacgg ctatccggca tacaacacca gtcccataac
    15961 gatcaccgcc acaaccatca atattaagct gatgattaat aagctgttgg tgtagggcag
    16021 gtcctctttc agacgcatcg ctttttcatt gcgtaaccag cgcaggtagc catacattgc
    16081 taacccgccg gaaaacagac acaacaacaa cgccagcagt tcacgaatga cgggcgtggc
    16141 gaaatctggc gcaagttgat caagcccaac ccccgccgcc agaaatccta gtgcggtacg
    16201 gatccacgcc agaaaagtac gctcattagc cagcgagaag cggtaatcgg gcgcttctcc
    16261 aaggcgggaa attttcattg ttgctccttt gcaatttcac taacgctcta tctttagcgg
    16321 ttttttttaa gaatttcgtc cgcccgtttc ctgatatgct tttgccatcc gctacaagga
    16381 ggtcagatgg gtatcatagc gcaaaataaa atcagttcat tgggcatgct gttcggtgcc
    16441 atcgctttaa tgatggggat tatccacttt tcattcggcc ctttttccgc accaccgcca
    16501 acgcttgaaa gcatcgtggc ggacaaaacg gctgaaatta aaagaggtct tctcgccgga
    16561 ataaaaggcg aaaaaattac cactgtggag aaaaaagagg acatggatat cgataaaatc
    16621 ctcgatcaaa gcggtatcgc actggccatt gctgcgctac tttgcgcatt tattggcggt
    16681 atgcgtaagg aaaatcgctg gggcattcgc ggcgcgctgg tttttggtat cggcatcgtg
    16741 tgcagcattt tgctgatatt cctgattttt tccttcctga ccggcggttc gctggtttaa
    16801 gcagcctgaa taatgcccgc cgggtgatca tcagcgggca ttatcgtcag aatttctgtt
    16861 ttttctcctc caccttcact ccttgcgtcg gtaggccggt gtcatagtcg cgtaccactg
    16921 gcgaatagcc atcttgtggg cgtgggcgaa acgcccccat ccagcgcggt cgtgcatctt
    16981 tacgccacgg acgcagactc cattggtaac tgcggaacgg atcgcgaatt ttgtccatgt
    17041 aatccaataa ggcgtcatgc attttgctgc gaacgtctgc aaaacggata tcatcgatca
    17101 ggttatgcat ttcatttggg tcattacggc gatcgtaaag ttcatcactg gtgaagaggt
    17161 tgagcaccag tttaaagtca tccgtcaccc agcaacgcac cggaataaaa ccgccaaagc
    17221 tgtcatgctc aatctcgtag cggttaaatt ccaccatcac gccgcgtggc tctttcacgg
    17281 caaggatatt ttcccccggc agaatctctg gtttttcaat atctgccagc gccatcattg
    17341 tcggcagtaa atcaatatga ctgactggcg tatcgacctg tcgccgctcc ccttgcggcg
    17401 aacggatgat cagcggaata cgggtgatgt cgtcatacat cgccgctcct ttactgatca
    17461 gcttatgtgc gcccatcatt tcaccgtgat cggaggtata aatgacccac gtattttcac
    17521 gttgctctgg cgttaaggca ttgatgacac gtccgatttg gtcatcaaca aagtcattgc
    17581 aggcaaaata gagcggatgg tgataaagcc cgtcatcacc gactggcgat ggcatcgcct
    17641 gcgcccataa gcggtgatgt tccggtttgt tcgccaggtc atcctgtgat ttctcgccaa
    17701 gttcgtagta aaaatcagcg tatttctcta aatactccac cggacaggtg aacgggtgat
    17761 gcggctcatc atacgaaacc accatcagga agggttcctc ggcgcgcacg ggctattgca
    17821 gaaaatctac cgctcgattg ctgatgcggt gcgcccaggt gaaggtttcg tcaatatggt
    17881 tcgcctgtaa atcttcgacg ctgtttaggc cattgcgcca caggctgatc tctttttccg
    17941 tcagttcgct aagatagttc gccccatcaa accagtaatc agcatcccac tccggcggac
    18001 actcgccagt gccgaaatag tcatgaccgt cgagatgcca tttgccgatg taacaggtgt
    18061 gatagccggc atccttaaag tagcgcccca tagtggagat gtttttgcct ggcgcgacgt
    18121 tgttggtcca cgggccggac tggttagcgt agataccggt gaatagtccg gcccgtgcag
    18181 gcgtacaaac cggtgaacaa gtgtaggcgg aattaaagcg aataccttcc gccgccagac
    18241 tatcaatatt ttgcgtattc agcggtttac cgctatagca accgaccata ttggtggcct
    18301 gggtatcggt caattggggc gtttcatagg ctatccttcg cgtcgggagt aaaggttacg
    18361 gctgggtcgt gacgatgcca ccagctgtca aaaatcaggt agacaatcac tacggcagcg
    18421 atgaaataac tgatcatcgc taaccagcgc gtaccgtagc cgccgaattc agccagcccg
    18481 acatagacgc caatcatcgc gaacaggata ccaattgacg cgatcttgac gtttttccac
    18541 ggtttcatgt ccaccgcaaa cgcatctttg aaggtgaacg gcgtggcgcg cggtttgata
    18601 aaaccgatca ccagcatcac gaccacgttg atgcagaacg tacaggccag cacatagagg
    18661 aaatggaagt cgaacttcac cagatagttg atggtgatgt agctgattat gccaatcccc
    18721 atcgccactt ttgccgccag cgccgggatg cgcggaaaga aaaagcccat aatgatgatg
    18781 gtaaccagcg gcacgttgta aatgccgttg agctgtttca tccagctata cagcccctgc
    18841 ggcgcgttcg cgatccacgg cgctaccaaa accgaaacga tagcgataaa gaaaccaaat
    18901 ttgcgcccga cggtgaccag ctgctgcggc tcggcattct ggttaatgat gcgacggtaa
    18961 atacccatac tgaataatgt actggcgcta ttcagaaagc cgttgaaggt actgatcacc
    19021 gcaccaaata acaccgcgcc gaagaaaccc tccagtggca ctggcagaac gttattgacc
    19081 agcgtcgggt aggccatgtc ggctttcggt agatcctgat acagatgaaa cgcaatcaac
    19141 cctggcagca ccagtaccag cgggtcgagc attttcagca ccgccgttaa cagcgccccc
    19201 ttttgccctt ccgccaggct ttttgacgcc agcgtgcgtt gcacgatgcc ctgatttgta
    19261 caccagtaaa aggtgttcac cagaattaaa ccggtaaatg ccgcgccaat cggcaaggga
    19321 tcggtcgggc caccgactga gtttaatttc tcggcgtgaa cggtggtaat ttgctcaatg
    19381 ccctgcataa agctgccctt gcctatcgcg atcaggccaa ataccggcac catcaacccg
    19441 ccgataacca gcccaatacc gttgatggag tccgccactg ccattgcgcg cagtccgccg
    19501 atcaccgcat acaaaattcc cgccagacca agcaaaatta ccagtagcca gatagccgca
    19561 ccgtgagaaa tctgtagcga ttccccgacg tgaaacaggc tgttgagcgc caacgcgccg
    19621 gagtagagaa caatcggcag aaagcagacg ccggtggcaa tgaggaagca gaagtcgatg
    19681 ataatacgcg tcgttttatc ataacgttcc tccagaaaat cggggatggt ggcaatgccg
    19741 cgcttcagat agcgcggtaa aaagattagc gcgaggaaga tcagcgtcac cgctgaagtc
    19801 acttcccagc ccatcaccga catgccgctt ttgtaggcct gcccggaaag accaaccagt
    19861 tgttccgtgg aaaggttggt taacattaac gaagcggcaa taaccggcgc ttttagtgaa
    19921 cgtccggcaa gaaaatagcc ttgttgcgat ccggtatctg ttttgcgaac cttccaccag
    19981 gtgatcaccg ccaccagcag cgtaaaaccg acaaaactca agatttgtaa cgaattcatc
    20041 tcatagccct tatttatgtt tatgtcccgg acgcatgaat atagggagag gtaaaatgcg
    20101 tcgggtcagg cagtcagatt cttaaaatac aaacgtcgta tccctgaacg gattgttttc
    20161 tgttaagttc aggttgtgag cataattcgc cggagggata ttaaaatgaa tggaaaattg
    20221 caaagttcag atgtaaaaaa cgaaactccg tacaatattc ccttattgat taacgaaaat
    20281 gtgatctcca gcggaatttc tctgatctcg ctctggcata cctacgccga cgagcattac
    20341 cgggtgatct ggccgcggga caagaagaaa ccgcttatcg ccaactcatg ggttgcggtg
    20401 tataccgtac aaggatgtgg gaaaattctt ttaaagaatg gcgaacaaat aactctgcat
    20461 agcaactgta ttatattttt aaagccaatg gatattcact cttatcactg tgaaggttta
    20521 gtctgggaac agtactggat ggaatttacc cccaccagta tgatggatat ttccgttggt
    20581 cagcaaagcg ttatttataa tggcgaaatt tataatcagg aactcaccga agttgctgag
    20641 ttaataactt caccagaagc aataaaaaat aatctggcag tcgcttttct gacgaaaatt
    20701 atttatcagt ggatttgtct tatgtccgca gatggtaaaa aagatccaca acggcggcaa
    20761 attgaaaaat taattgccac tttacatgcc agtctgcaac aacgctggag cgtagctgat
    20821 atggctgcca cgatcccctg tagcgaagcc tggttgcgtc gtctgttttt acgctatacc
    20881 ggcaagacgc cgaaagaata ttacctcgat gcgcgtctgg atctggcgct atcgctatta
    20941 aagcaacaag gaaactcggt tggcgaagtc gctgatacgc tcaacttctt cgactccttt
    21001 catttcagca aagcctttaa acataaattt ggttatgcgc cgtcagccgt gctaaagaat
    21061 acggaccagc acccaacgga tgccagtcca cacaattaac gtaactctgg ccagtacgct
    21121 ttgttggcct ccagcaactc atccagaata gctttggcga ccgaggcgct cggtaccgtt
    21181 ttcgacaacg taatcgcctg ccacaggtgc tgatatgaac gctgttccca ggcgtccacg
    21241 accagttttt ccaccgccac ttgctgactc attaaccctt tctgaaactg cgggatatcg
    21301 ccgaccacca gtggttcagg ccgtagtagc gcacgcgcat ctgtttgcga tcttgcagcc
    21361 caacaatttg cgccatccgg ctttcaatac cgattggcat gtcacagatg ttgaggattt
    21421 tcgcattcgg gcgcagacga cgcgtggctt ctgcgacaat tgctgccggg ttggagtagt
    21481 tgagcatcta ggcgtttggt gaatattttt ccatataatc caccagttcc aggacgccgc
    21541 caatggaacg catgccgtac gctattccgc cgggtccgca agtttcctga ccaacaacac
    21601 cgtggcgcag tgggattttt tcatccagtt cgcgcatcgg gtacttgccg acgcggatgt
    21661 gcgccatgac aaaatcaacg tcgctgaacg ccacttcagg atcggtggtg taactaaacg
    21721 caatgtccgg cgctttttct ttaaggatga ctttacaggc ttcggcaatc acttcctgcc
    21781 gcgcgccatc gttatcataa aatttcagtg cacgaagcgg gaaacggtcc tgattcgcca
    21841 ggagcatcaa cacgatgcct ggcgtaaagg tgcttccacc gcctgcgaca accactgaga
    21901 atttggtcat tatactgcct ccgtaatggc aacattttct gctgattgat gagaattaat
    21961 taagctatcg agctgttcac gcaattggga tacatgcaga ccaatgatca cctgaatggc
    22021 atcgccacta cggaagacgc cgtgcgctcc cagcttttta aagacttcgt catccagcgt
    22081 ttgtgacatg tcatgcagtg caatacgtag acgcgtcgcg caattgttaa tgctggagat
    22141 attgccgacc ccgcccaggg cttgcaggat accggcagcc tgatccagct ctttttttgg
    22201 ctctgcagcg gtggtttggc ctcgcgaggc tttgtattcg gcttttgagt agagtttcac
    22261 ttccgcatct tcacgtcccg gcgtgcacat gttgaactgc agaatcaggg tgcggaaaac
    22321 cacgaagtac agcagggtaa agcacaaccc aatggcgatt tgggtcagca tcatatccgc
    22381 gtggttgctg aacatcggga tccagttttg cggtaaaacc tggtcaatca gacctccgcc
    22441 catgttgccc accacaccaa agagatacat tacggtcgac attgaggccg ccagcacggc
    22501 gtgtaccgca aacagcaacg gtgaaatgaa caggaaggta aattccagcg gttcggtaat
    22561 tcccaccagc atggcggtta aggttgcggg aatcagcaag cccgccactt ttacccgatt
    22621 ttccggtgcg gcagtgaagt acatcgctaa agaaatgccc acggcaccaa agatttttga
    22681 gttaccgtgc agggcaaaac cgccttccgg gaacaacgat ttcagtggct cggcgctcag
    22741 actgaactct tgcagatgct gcgcccagta catctgaatg ccgccttcaa cagctgccgg
    22801 accaaagata aacggtccgt agatgaagtg gtgtaaaccg gttgggatca gaatacgttc
    22861 gaggaaggtg taaacccaca ccccaagcgc acccgccgaa cgcaggaacg cttgcagaga
    22921 ttcaatcccc atttgtactt ttggccagcc gagcaatgtc agccaggcac aggggatcat
    22981 caccaggaag gcgataatca ccacataaga cgtcccctgg aaaatgccga gaaaaaccgg
    23041 cagtttttta tcgaacagac ggttatgcag cgccgtcaca atgccggaaa tgataattgc
    23101 gccgataatg ctggtatcga gggttttaat cccggccatc attgtcagac cgctacctgc
    23161 caccgcgtcc tgagtgaaat cgacgccgaa gtagcttccc caggtcattc ccatcgcatt
    23221 gatgaaatag ttccaggtca ggaaactcac catcaccgcc agacaagcac gtccctgcgc
    23281 ttgcttagca aggccaatgg gtaaaccgac agcaaaaatc agcggcatat tacggaatac
    23341 cgtccaaccg ccctcttcaa taatgtgtac gatttgcgcg aataaactgt tcggatcggt
    23401 cagtgattcc ccgacaaaca tcgggttttg cagcaagatg gcaagaccca ccacaatccc
    23461 ggcgaaggga aacagcagca ctggcgtgaa catcgcgccg ccaaagcgtt gaatttgact
    23521 gagcatttcc acatcctctt ttctcaattc tgtaagtagg aagcctgatt ggttatgcgg
    23581 cagacctgga gcgaggatag gaagatgcga tcggcagaac atgacgaccc aatgggattc
    23641 gtgatcgctt tcatgctttt cgttgggcgg tagagatcta cgttttcgcg aaaaagtaga
    23701 catctgtgag cacaggtaca cagcatcgaa cacatcttta aaaaaaagat ttttttcaat
    23761 cgattaagca gaacttgtgg gcgcattacc cgtgcttgca ggcaaaaaag agagctagag
    23821 atgatctaca aaagcattgc ggagcggtta agaattcgac ttaattccgc ggatttcacg
    23881 ctaaacagcc ttcttcccgg tgaaaaaaag ctggcggaag agtttgcggt atcgcggatg
    23941 actatccgta aagccattga cctgctggta gcgtgggggc tggtggtccg ccgccacggc
    24001 agcggcactt acctggtgcg caaagatgtg ctgcatcaaa ccgccagcct gaccggactg
    24061 gtggaggtgt taaaacggca gggaaaaacg gtcaccagcc aggtgctgat ttttgaaatc
    24121 atgcctgcgc ctccggccat tgccagccag ttacggattc aaatcaacga gcagatctac
    24181 ttctcccgtc gcgttcgttt tgtggaaggg aaaccgctaa tgctggaaga cagctatatg
    24241 ccggtaaaac tgttccgtaa cctttcgctg caacatctgg aagggtcgaa gtttgaatat
    24301 attgaacaag agtgcgggat tttgattggc ggtaattatg aaagcctgat gccggtgctc
    24361 gccgatagac tgctggcgcg gcaaatgaag gtagcggaac acacgccact gctgcggatc
    24421 acctcgttgt catatagcga gagcggggag tttttgaatt attcagtgat gttcagaaat
    24481 gccagcgaat actaggtgga gtaccattta cggcgactcc acccggaaaa aagttaaccg
    24541 atactccaga agagcaccgc cagtaattgg ggggtgataa tgcgcaggaa catcactaac
    24601 ggatagacag tggcgtaaga gagcgccgcc gcaccactgg ttggatgaag attattagca
    24661 aacgccagcg ccggaggatc ggtcatggaa cctgccagca tcccgcacat ggtcaggtaa
    24721 ttcattttgg ctaacatccg cgccagaatg ccaacagtaa tcagcggaac ggcggtgatc
    24781 agggcaccat aaccaatcca gcttagccct tcgccattga ccagggtatt cacaaaatcc
    24841 ccaccagatt tcagaccaac gaccgagagg aacagcacga tccccagctc ccgcagcgcg
    24901 aggttggcac ttggcggcat aaaccagtac agcttgccga tactgccgat acgcccgagg
    24961 atcaacgcca taatcagcgg accgcccgcc agccccagtt tcaacgcggc cgggaatcct
    25021 ggcacaaaga cgggaataga acctaacaat acgccaagcc cgatgccaat aaacactggc
    25081 agcatctgaa cctgttgcag tttttgctgc gcattcccca gcacattggc aacggcatca
    25141 attgcggacg gacgccccac caggttgagg atatcgccga actgcaggct gatatcgcca
    25201 ctggcgacca gttcgacccc ggcacggttc aggcgcgaga taacaacgtc atagcgttct
    25261 ttaaagtgca ggtcgcgaat acgttttccg agcacgtttt cattggtgac caccacacgc
    25321 tcgacgcgca aatcagtgcc tttcgtagac agcgaggtat cgacctcctg accaatcacc
    25381 agttgcgcat tatgtaaatc cgctggctgc cccaccagat gcagcaaatc gcccagttgg
    25441 ataatggtat ctggcgaagg tacttttagg gtttcttctc gtttcagacg cgagcagata
    25501 attttgtcgc cgttgagaat cggtacatct ttaatggcta aatcatgcag gttagggttc
    25561 tcaacgcgaa tattgatagt cttgatcagc gcgccgccat tggtgcgtga agactcgtgc
    25621 tgctgagctt ctgtctcgac attgacgcgg aaaataaccc gcaacatcca catggtgaac
    25681 aaaatcccgc aaatgccgaa tggatacgcc atcgcgtaac tcatccccat ctgatcgacc
    25741 atttccattg gtgtacccag gtcgcgcaag atctgctgcc ctgcccccag cgctggcgta
    25801 ttggttaccg caccggagaa aatccccagc actaccggca gtggaatatc aaacagttta
    25861 tgcaggatgg cggtaaccag accaccgatg atgacgatca gaacagcaaa caggttgagg
    25921 cgtaatccgg agacgcgcaa tgaggcaaag aagcccggcc ctacctgaat cccgatggta
    25981 taaacgaaca ggatcaggcc aaattcctga ataacatgca gcatatcgct acttaatgtc
    26041 atccccgcct gagaaacaaa atggccgacg atgatcccac caaacagcac gccgccaata
    26101 cctaatccta tgccgcgaaa tttgacgttg ccgataaaca aaccgacgac tgccaccaaa
    26161 gccagaatac tgaccgttaa tgctatatca ctcatcgtcc atttctcttg agataacatt
    26221 ttaattgcta cggattatgg ctgaatcggt gaagcagatt tgccttatag cgcacattat
    26281 cacgggaaag aacgtgccga aatatcttaa acagtcgaaa tttaacgcat tgttgaagaa
    26341 gaaagacaaa aaaagcccca ccagaatggc ggggcaaaga gaatagctag ttagctattt
    26401 aacgcgggac gttcgctgat agcgatacgc tgcgctgcga tgggttcagg ctcattacga
    26461 attaaatcaa tatgcagtaa accgtttacg aaggttgcgc cagagacttc catattttca
    26521 gccagcgtaa aactcaggct aaatggctga ttcataagcc cttgatgcag ccattttttc
    26581 tcttcttttg gctgctccgg cgtgcctttt acgctcaggc gcgtgccttc cagttgaatc
    26641 tctaaatctt cctgacggaa acctgccagc gcaagggtaa tgcggtagtg gttatcgtcg
    26701 cttttctcaa tgttgtacgg cgggaagctc tggctttcac cggcgttttg cagtgcgttg
    26761 gccagtttgt caaaaccgat ccattgacgc atcagtgggg ataaatcgaa gttacgcata
    26821 gtcatttctc cttctaagaa gcgagtaagt acctgcaaat ccgaagattc gcatatgctc
    26881 cctgacggcg agcatggaga tgtcaggccg cgccaggcgg ccttagggaa ttagttgatt
    26941 tcgatacggc gcggtttttt cgcttccgga atcacgcgtt cgagatcgat atacagcaaa
    27001 ccatttacca ggttagcccc acgaacatga atgttctcag ctaactggaa tttgcgttca
    27061 aagttgcgtt cagcgatgcc ctggtacaga taggtgcgct ctttttgttc gtcggcgtga
    27121 gcacctttca ccaccagcag attatcctgg gcggtaattt ccagttcgct ctcagcaaaa
    27181 ccagccacag cgatagcaat gcggtaatgg ttttcgtcta ccagttcaac gttatacgga
    27241 gggtagccgc cattactctg gctctggttg ttttctaagt ggttaaacaa acggtcaaat
    27301 ccaatagcag aacggtaaag cggggataaa tcaaagttac gcataatcaa tagctcctga
    27361 aatcagcgag aatgtaagac cttccacaat ggacaggtca ggtagccaga acacccatca
    27421 ggcgcgtcct catcgactac gatgtaaaat gggtctggaa atgaactttt caagccttat
    27481 cagcggactt ttttttgcag tttatggtct attgcataga ctgagggggc agcacgaaac
    27541 gttaaagtgc gatagccgcc acagtgcgac gtaatgatga tgttattttc agcaagggat
    27601 cgctataact catcaggcca acacccacga tcaacagatg aatttattat gataagaaat
    27661 gtgttgttag cgttcatgat atgcagcgga atgacattac tcggaggatg ctccagcgtg
    27721 atgtcccata ccggggggaa agaaggaacg tatcccggca cccgcgccag cgccaccatg
    27781 attggcgacg atgaaaccaa ctggggcact aagtcgctgg cgattctcga tatgccgttt
    27841 accgctgtaa tggacacgct tttactgccg tgggatgtgt tccgcaaaga cagctcggtg
    27901 cgctcgcgcg ttgaaaaaag cgaagccaac gcgcaagcca ccaatgcggt tattccaccg
    27961 gccagaatgc ctgataactg atttagcgat cagtttcggt gatccacagc tggccacctt
    28021 ccatccacgc cagccattga ccatccggcg agaagaccac ggcgtccgca gaaggtggat
    28081 tggcgtggtt ttcggttaaa tactcaacct cgccgctttg cgcatgggca caaacaattc
    28141 gattatccag cacaaagccc aaccattctc ctgacggatg ccagttaaat gcagactgaa
    28201 tatccgtttt gttatgggtt aacttgcgcg gttcgccgcc ctgtggcgag ataagccaca
    28261 gttgcacaat gccgttatca tcacgcatta aaaacgcgat ttgcgtaccc tgcggattac
    28321 agcgcaccca gtggcgcggg acgttgacta accccggata agcccgatga tgggtaaagg
    28381 ttaaacgtcg ctgcacgacg ccacgcggtg gcgcgggtag ggttgtttcc gttccactta
    28441 acggcgcatc acctgccgat ttccagctag cttcatcttg cggtaactca acgataaata
    28501 gctccggcac tttctcgccc tttggcgaaa gtgtgtcgcc aataaacgcc agcgcgtgat
    28561 ttcctaccca tccttcttca taagcacgat tgatttcatc actgccaggc tgcggcgtgg
    28621 gcgtggtttt actcaccagc acacaccagt ggctaccgct gtattcacgc ggatgttgct
    28681 tttgtacgtt gaccgggcca aacggtgcag caaccccgac gtttcgcaaa tccagcgccg
    28741 gatcgagttg atgcattaca tggtcgttat aggtaaagct ccccctttca ccgttcgggc
    28801 taaagacatg cacatggctg ccaccgcgca gcgctcctgg cgtatacggc gcggtaatat
    28861 ccattgcatc gagattgctc accttgccgc cttcagcaat gactccgcgt cgatgatgga
    28921 agtcgtaatg ccatgtttca tcaggatttt cagggccgtg aataaagaca tatttctctg
    28981 acttcggatg aacggtcacc acgccgacgt gtgcgccctg tgacgcgcga tagataacct
    29041 cgacctcgcc tgtatggata ttcacacgct caatggtctc gccggtaaac gacgccccag
    29101 aaggacgcac gtcaaatacc agccactggc tgtcgggcgt ccaggtattg gtatttgtat
    29161 tggtattggt attggtgagc aggtgattac ggggagcaaa ggttatctgt ttcatggcgc
    29221 ggtcctgata caaaacggtt cccgccatca tacttcacaa ggacttcact ttcagacaat
    29281 agtcttttgc catcgtgatg aatgtgagtg aaaagatgga acatttcgac gtggcgatta
    29341 ttggcctcgg cccggcaggg tcggcgttgg cacgaaagtt agccggcaaa atgcaggtga
    29401 tcgcgctgga taaaaagcac cagtgcggta ctgaaggttt cagcaaacct tgcggcggtc
    29461 tgctggcacc ggacgcgcag cgatctttta ttcgcgatgg actgacgctt cctgtcgatg
    29521 tgatcgccaa tccacagatt ttcagcgtca aaactgtcga cgtcgccgca tcgctcacgc
    29581 gtaactacca gcgaagctat atcaatatta atcgccatgc tttcgacttg tggatgaaat
    29641 cgctgatccc cgccagcgtt gaggtttatc acgatagcct gtgccgaaaa atctggcgtg
    29701 aggatgataa atggcatgtc atttttcgtg cagacggctg ggagcaacat attaccgccc
    29761 gctatctggt cggtgccgat ggcgcaaact cgatggtgcg gcgacatctc tacccggatc
    29821 atcaaatccg taaatatgtc gctatccagc agtggttcgc agagaaacat ccggtgccgt
    29881 tctactcctg catctttgat aatgcgataa ctgactgtta ctcatggagt atcagcaaag
    29941 atggttattt tatctttggc ggtgcctatc caatgaaaga cggtcagacg cgtttcacga
    30001 cgctgaaaga gaaaatgagc gcctttcagt tccagtttgg caaagcggtg aaaagtgaga
    30061 aatgcacggt gttgtttccc tcacgctggc aggattttgt ctgtggtaag gacaacgcct
    30121 ttctgattgg tgaagcggcg ggatttatca gcgccagctc gctggagggg attagctatg
    30181 cgctggatag cgcagagatt ctgcgtgcgg tgttcctgaa acagccggag aagagcaacg
    30241 ccgcttactg gcgcgccaca cacaaactgc gtttaaaact cttcggcaag atagtaaaaa
    30301 gccgatgcct gaccgcaccg gctttaagaa agtggattat gcgcagtggt gtggcgcata
    30361 ttccacagtt gaaagattat ccaacgcgct tcacatcacc caccagcagg atgtaagaga
    30421 gcgcgccaat caacgcgacg gcggagatat aaaccagtgc aggcgcgaaa ccgtaaccct
    30481 gcgccaggta ccccaccacc agcggaacgg tgatgccgcc cagaccaccg gcgaagttaa
    30541 acacgccgcc ggttaaacca atcaggcgca tcggtgccag agaagagacc agcgaccagg
    30601 taatcgaagc aaaaccgttg ccgaagaatg ccagcgccat caggcacata atcatcatcg
    30661 gatcgttggt gtagttagcg cccataatgc aggtggagat cagcaagccg cagataatcg
    30721 gcgttttacg cgcaaagccc agtgaaaagc ccttacgtac cagcagatcc gcgacccagc
    30781 cagagagcag gacgccgaca aacgccgcga ggaatggcac cgtggtcata aagcctgctt
    30841 tcagcgccgt gattcctttt tcctgggtta aatagttcgg gaaccaggtt aagaaaaacc
    30901 acagtgcaga agccaccgca aattgcccaa gataaacgcc gatcagttta cgatggaaca
    30961 ccagtttcca gtctttggct gttaacggct gacgcgcctc tttcttcacc ggcgcatcac
    31021 catccaccag accgccgcca tcacgaatgt aatccagttc agctttgctg atacctttgg
    31081 tcaggcgcgg cggctgataa accttaaacc aaatcagcga ccagataatg ccgataccac
    31141 cagtgacaat gaacacccag tgccagctca acatctcctg aatccagatc agcagcggag
    31201 tcaaaaacgc cagaccgaca aactgaccag acgtataaaa accaacggcg gaagcgcgtt
    31261 catgttccgg gaaccagctg gtcaccatcc ggttattggt cgggaaagcg ggcgcttcga
    31321 aaataccggt tatcgcacgc agaccaatta atgacattaa tcccgtggca aagccttgga
    31381 ataaagtcgc cactgaccaa ccaaatatcg caataaaata agtcacgcga gaacctacgc
    31441 gatctaaaaa ccaaccgccg gggatctggc atagcgtata aagccaggcg aaggccgaaa
    31501 atacatagcc catttccgct ttcgtaatgc cgaactcttc ctgaatatgg gcggaagcca
    31561 cggccaggtt agcgcggtca acataacaaa tgaccaccgt aataaagatc atcaccagcg
    31621 tcagataacg ccgacgcccc ggctttgctg cattaacggg aatatccata gcgagctttc
    31681 tccagatttt gggcatagcg aagccgctca ccatgccctg taatttacag agggttatat
    31741 ttttgtattg ctgttttagt gcccgatgag gggcttacgt ggcaggaatt accactctgc
    31801 tacgctgtta tcttcatgac gccagagcgg attacgccag tccggggcat ttttactgaa
    31861 ctcaataact ttagcttcgt cgatttccac acctaagccc ggtttcgtta acggtttaaa
    31921 gaagccgcca accatgctga agtcttcttt gtttttcaca aagtcgagta actccgcgcc
    31981 tttgttgtaa tgaatgccca tactttgtta ctgaagtacc gcgttatagg aaacgaagtc
    32041 gatatgcagg caagccgcca gtgcaatcgg tccgaacgga cagtgcggcg caagggtcac
    32101 atcataggct tctgccattc cagcaatttt gtagcattcg gtaataccgc ctgcatggga
    32161 gagatccggt tgcagaatcg aaataccgcc tgcctccagc acgcgtttaa aatcgaaacg
    32221 tgagaacatg cgctcacccg ccgccagtgg aatatgcgtt tgtgccgcca atttcgggta
    32281 gtattcggct tgttccgcca gcaccggctc ttcaataaac agcgggcgat acggctccag
    32341 ttctttaatc agcactttcg ccattggcgc gctgacgcga ccgtggaaat caagaccaaa
    32401 ctcaatctga ttgccaaaag cttcacgaat ttgtgccacg gtgttgactg ccgcatctac
    32461 cgcgcgggag ttatcaatta gccccagttc ttcacaaccg ttcagtttga aggtatcgaa
    32521 gccgatttcg cgcagggttt taatgccgtc gataacatcc gccggacgat cgccgccgac
    32581 ccaactgtag gctttaattt tgtcgcgaac caggccgccc atcagttgcc agactggcgc
    32641 attcagcact ttgcctttga tatcccataa cgcctggtcg atcccggcga tggcgctcat
    32701 caggattgga ccgccacgat aaaatccggc gcgatacatc acttgccata agtcgttgat
    32761 gcgcgaagga tcctgaccaa tcaaatagtc acccagctcg tgaactgccg cttccaccgt
    32821 acgggcgcgg ccttcaatca cgggctcgcc ccaaccgacc acgccttcat cggtttcaat
    32881 tttcaggaac atccagcggg gaggtaaacg atacgtggta attttggtga ttttcattgc
    32941 actgcctctc gatacgcctt aacaaatgct gctgcctgct gcgcggtgcg ttctacggac
    33001 tgcccggcgc gataaagatc gctgcctaag cccgccccaa cacaacctgc gtctatccac
    33061 tgcgccaggt tttctggcgt cacgccgcca acggcaaaga ctgcgatgtc cggtggtaat
    33121 accgctttta acgctttgat gtattgcgga ccaaaagccg atgacggaaa tattttcagc
    33181 gcctgcgcgc ccgcttcgag cgcggtaaag gcttcggtcg ccgtcgcgca gccggggcaa
    33241 acggtcatgc cgtagcccac cgcgcggcgg atcacttcac tatggatatt gggcgtaacg
    33301 atgagctgac agcccatcct ggcgagcgca tcgacctgtt caggtttcag taccgtacct
    33361 gcgccaatca acgccttgtc gccatacgca tcaacgatgg cgggaatgct ctgctcccat
    33421 tgtggggaat tcagcgggat ttcaaccgcg tcgaacccgg cgtcaatcac cgcgccaaca
    33481 tgcgccagcg cctcgtcggg cgtaatgccg cgcaaaatgg cgatcagcgg gagtttagtt
    33541 tgccactgca tgagcgatgc tccttatacc agcctgaaat gccgtgtcgc ccgccaccgc
    33601 cgccacgtcg caacccatcg cctgaaaggc ttgctggtag cgcgcggtca gcgatgttcc
    33661 ggcgacaagg gtgatggcgt gttgatgggt cacatagtcg cgcatactgg caacctctgc
    33721 gccaatcaac aaaccagaga gaaattcgct gacctgttcg cggggaagtg ttcccagcac
    33781 atgcgaggcg cgaacttcaa aaagctgcgg caatatggcg ggcgcattaa ggccgcgctc
    33841 aaggccagcc gcgaaggcat cggcagagtt ttcctgcggc ggcaaacctg cgccaatcaa
    33901 tgagtgattt aacagtaaat gatgtaattc accggtcatc acggtgcgaa aatcgttgat
    33961 ttgctggcta tcggcctgca cccatttgca atgcgtaccg ggcataacat aaagagagga
    34021 aggagccaga gctcgcgcgc cgatcaattg tgtttcttcg ccgcgcatca cattgtggtt
    34081 atcgtcatga gagacgcata atccgggaat aatccagata ttgtcgccaa cagacgttaa
    34141 ttgttcgcca atagacgaaa aacgggcagg aacagataaa tacggagcaa ctttccagcc
    34201 gacattgctg ccgaccattc ctgccatcac cactggcgta ttttcttcac gccagtcggt
    34261 cgtgacttct gctaacactg cagccggaga ttttccgttc aggcgcgtga cgcctgcttc
    34321 tgattgcctg ctctccaggc agtggtcgcc ctgataaagc caggcgcgca gattggtcga
    34381 tccccagtca attgcgatgt agcgagctgt catgtgattt cctttaacct tcgtgtcgag
    34441 ctggcaatca tggtaagcgc cgcctgctct gccgcatcgc cgtcctgatg ccgtatcgca
    34501 tcgaacagcg ccttatgttc ctggagcgtt tgcggcatgt tggcctcatc gcccatccag
    34561 gttcgttcaa aaaccgcccg ctgcagcgaa ctgatcgcaa tgctaagttg ctgtaacacc
    34621 gggttatgca ccgactgcag caccgcctcg tggtagcgaa tatccgcttc gttaaacgct
    34681 tcgcggtcct gattgttggc aatcatctcg ttcagcgccg attcaatctg cgccagatcg
    34741 ctggaggtcg cgcgctctgc tgcccaacgg gcaatcgccg gttccaccag atttcgcact
    34801 tcgctcatgg cactgataag ccgtgggtcg tagtcgtttt ccagcaccca ttgcagtacg
    34861 tcagtgtcga ggtaattcca ctggttacgc ggtgccacaa acgccccgcg ataacgtttc
    34921 atttcaatca gccgcttcgc catcagcgaa cggaacacct cacggatgat gttgcgcgag
    34981 gttgcaaact cttcacagag ctccgcctca gccgcaagtg gcgagccagg tacgtatttg
    35041 ccgtgaacga tctgtttacc cagcgtaatg acaatgcgat cggttttatt gagagtcatg
    35101 gagagtcctt gtgctctgta tgttcttctc tactttaccc cgatcgatgc ataacgcggc
    35161 aactttgtag taccagcgtg atgacgttcg cgtttgccgc gcgcgtaatg tagtacaaac
    35221 ttatattgtt gcgctacaat ttagatcaaa aaaagaacaa cacataaaaa aatgacatgc
    35281 gtcgggcaga aatctgaaaa gggatatcag gcgctaaaca ggagggaaag aagagtatgc
    35341 tttcaacggc ttagctactc gtttaaagga ttaatcatga agttgaattt taagggattt
    35401 tttaaggctg ccggtttatt cccactggcg ctgatgcttt caggctgtat ctcgtatgct
    35461 ctggtttccc ataccgcaaa gggtagttca ggaaagtatc aatcgcagtc agacaccatc
    35521 actgggctat cgcaggcaaa agatagtaat ggaacaaaag gctatgtttt tgtaggggaa
    35581 tcgctggatt accttatcac tgatggtgcc gatgacatcg ttaagatgct caatgatcca
    35641 gcacttaacc ggcacaatat tcaggttgcc gatgacgcaa gatttgtttt aaatgcgggg
    35701 aaaaagaaat ttaccggcac aatatcgctt tactactact ggaataacga agaagaaaag
    35761 gcactggcaa cgcattatgg ttttgcctgt ggtgttcaac actgtaccag gtcactggaa
    35821 aacctaaaag gcacaatcca tgagaaaaat aaaaacatgg attactcaaa ggtgatggcg
    35881 ttctaccatc catttaaagt gcgattttat gaatactatt cacccagagg cattccggat
    35941 ggtgtttccg cagcattact gccagtgact gttacactgg acatcattac tgcaccgctg
    36001 caatttctgg ttgtatatgc agtaaaccaa taatcagtaa gcgggcaaac gcgtttatgc
    36061 tgtttgcccg cccacagatt aattcagcac atacttctca atagcaaacg ccacgccatc
    36121 ttcaaggttg gattttgtga caaagttcgc cacttctttc accgaaggaa tagcgttatc
    36181 catcgccaca ccgacgcctg catattcaat cattgcgatg tcgttttcct gatcgccaat
    36241 cgccatgatt tcttccggtt taatacctaa cacgtcggcc agtgatttca cacccgtacc
    36301 tttgttaacg cgtttatcga ggatttcgag gaagtacggc gcacttttca gcacggtata
    36361 tttctctttc acttcctgcg gaatacgcgc gatagcctgg tcgaggatgg cgggttcatc
    36421 aatcatcatc actttcagga actgggtatt ggggtccatt ttctccgctt cgcagaacac
    36481 cagcggaatt gtggcaacga aggattcatg caccgtgtag tagctgatat cgcggttagc
    36541 ggtgtacagc gtggtgcggt ccagggcgtg gaaatgagaa ccgacttcgc gagagagttt
    36601 ttccaggaaa cgatagtcgt catagctgag agcggtttgc gccacggtgc taccatcagc
    36661 ggccttctgt accagcgcgc cgttataagt aatgcagtag tcgcccggct gttccatatg
    36721 cagctctttc aggtagttgt gcacacctgc atacggacga cccgtcgtta gcacgacatt
    36781 cacgccacgg gcgcgagctg cggcaatcgc atttttaacg gcgggtgaaa tggtgtgatc
    36841 gggcagcaga agggtgccat ccatatcgat agcaatgagt ttgatagcca tgagttccca
    36901 aggtagatta gttcctgacc catgctaacg cgattccgct caaaaatcag tacaacaccc
    36961 gagggaaaag gggatgcaac gcgggttgca tgctccccat ttgcttagcg gaagagtttc
    37021 cctttcagca gttccatgcc tgcggaaagc agatcgttat tggcttgtgc tgacacttca
    37081 ccttgcggtg agagcgcatc aataatcttc ggcaattgct ctgccagtaa actggaagcc
    37141 gtactggtat ccacgccaag tttttgcccg agatcggaca ccgcatttgt gccgagcgcc
    37201 gactccagtt gctcgccgct aactgattga tttctctgtt gattactcaa ccaggttgag
    37261 agaatggccc ctaagccgcc actttgcagt ttttccagca gcacctgaat gccaccctgc
    37321 tcctcaaccc aacttaaaat cgcctgatat ttccccgcat cgcctttcag aaaggcaccg
    37381 acaacttcat caaaaagccc catgataatc acctgtaaag cgttacgtgt tgacccaaaa
    37441 agtatagatc tgcgggatga taattgcgga ttgcagaaat aaaaagggcg gagatgatct
    37501 ccgccctttt cttatagctt cttgccggat gcggcgtgaa cgccttaccc ggcctacaaa
    37561 atcatgcaaa atcaatatat tgcaggagtc tcgtaggact aataagcgta gcgcatcagg
    37621 cacgctcgca tggttaacgc cattaaatat caatattcgc cgctttcagg gcgttctctt
    37681 caataaacgc acgacgcggt tctacggcgt cgcccatcag cgtggtgaac aactggtcag
    37741 cggcaatcgc atccttaacg gtaacgcgca gcatacgacg gctttccggg tccatggtgg
    37801 tttcccacag ctgttccggg ttcatctcgc ccagaccttt ataacgctgg atggagaggc
    37861 cacgacggga ctctttcacc agccagtcca gcgcctgctc gaagctggct accggctgac
    37921 gacgctcgcc acgttcgata aacgcatctt cttccagcaa gccacgcagt ttctcaccca
    37981 gcgtgcagat acgacgatat tcgccaccgg tgataaactc gtgatccagc ggatagtcag
    38041 tatccacacc gtgggtacgc acgcgaacaa tcggctcgaa caggttttgt tcggcattgg
    38101 tgtggacatc aaacttccac tggctgccgt gctgttcttt gtcgttcagt tcgctgacca
    38161 gcgcgttcac ccagcgggta acggtctgct catcagaaag gtcagcttcc gtcaacgtcg
    38221 gctgatagat aagctctttc agcattgctt tcggataacg acgctccatg cggttgatca
    38281 ttttctgcgt cgcgttgtac tcagatacca gtttctctaa cgcttcgcca gccaatgccg
    38341 gtgcactggc gttggtgtgc agcgttgcgc cgtccagcgc gatagagatc tggtactgat
    38401 ccatcgcttc gtcgtcttta atgtactgtt cctgcttgcc tttcttcact ttgtacagcg
    38461 gcggctgagc gatgtagacg tgaccgcgtt caacgatttc cggcatctga cgatagaaga
    38521 aggtcaacag cagcgtacga atgtgcgagc cgtcgacgtc cgcatcggtc atgatgatga
    38581 tgctgtgata acgcagtttg tccgggttgt actcgtcacg accgatacca cagccaagcg
    38641 cggtgataag cgtcgccact tcctgagaag agagcatctt atcgaagcgc gctttctcga
    38701 cgttaaggat tttacccttc agcggcagaa tcgcctggtt cttgcggtta cgcccctgct
    38761 tcgcagagcc gcccgcggag tccccttcca ccaggtacag ttcggaaagc gccggatcgc
    38821 gttcctggca gtctgccagt ttgcccggca ggccagctaa gtcgagcgca cctttacggc
    38881 gggtcatttc acgcgcgcga cgcgccgctt cacgggcacg ggcagcatcg ataattttgc
    38941 caaccacgat tttcgcatca gttgggtttt ccagcaggta ttccgccagc agttcgttca
    39001 tctgctgttc aaccgccgat ttcacctcag aagaaaccag tttgtctttg gtctgggagg
    39061 agaatttcgg gtccggcact tttacggaaa cgaccgcaat caggccttca cgcgcatcgt
    39121 caccggtggc gctgactttg gcttttttgc tgtagccttc tttgtccatg taggcattca
    39181 gggtacgggt catcgccgca cggaagcctg ccaggtgagt accgccgtca cgctgcggaa
    39241 tgttgttggt aaagcagtag atgttttcct ggaagccatc gttccactgc agcgccactt
    39301 caacgccaat accgtctttt tcggtggaga agtagaagat atttgggtgg atcggcgttt
    39361 tgttcttgtt cagatattca acgaacgctt tgatgccgcc ttcatagtgg aagtggtctt
    39421 ctttgccgtc acgcttgtcg cgcagacgaa tggaaacgcc ggagttgagg aacgacaact
    39481 cacgcagacg tttcgccaga atttcatatt cgaactcggt cacattggtg aaggtttcga
    39541 ggctgggcca gaaacgcacc atggtgccgg ttttttcagt ctcgccggta accgccagcg
    39601 gagcctgcgg tacaccgtgt tcgtagatct gacggtgaat tttaccctcg cgctgaataa
    39661 ccagctccag tttttgcgac agggcgttta ctaccgaaac accaacgccg tgcagaccgc
    39721 cggacacttt ataggagtta tcgtcaaatt taccgcctgc gtgcagaacg gtcatgatca
    39781 cttccgccgc cgatacgccc tcttccgggt gaataccggt cggaatgccg cgcccgtcat
    39841 cctgtacaga gacagagtta tcggcgtgaa tggtgacgat aatttcttta cagtgacccg
    39901 cgagcgcttc gtcgatagcg ttatctacca cctcgaatac catgtggtgc agaccggtgc
    39961 cgtcatccgt gtcgccgata tacatacccg ggcgcttacg caccgcatcc agccctttca
    40021 ggactttgat actggaggag tcataagaat tcgacatcaa cgtttctcgc tcatttatac
    40081 ttgggttaat ccgttatttt acccttttcc acggtaaaca tcttcgaatt ttcgtccgac
    40141 atgtctataa cgtgttcagc actgatcgcg ctgacaaaga cctgtgattg cgtcgctttt
    40201 aagcggctgg caagcaaccc gcgacgctca tcatcaagct cagaggcaaa atcatctatc
    40261 aggtagagac accgccgccc gctttcacga gtgaggaact ctccttgcgc cagacgtaag
    40321 gcgcacatca acagcttaag ctgcccacgc gataaggtat cttccaccgg cgcaccgtcg
    40381 gcgcgaatgc gtaagtccgc tttatgcggg ccatgcgcgg tgtaggttag ctggcgatcg
    40441 cgttcaaaat tacgttccag cacctcagcg tactctgtct ctttctccca gccgcgctgg
    40501 aaagagaaag tcagagaaaa ctcagggaga aattgcttac aggtatcatc catatcggcc
    40561 gcgataccgg cgctatactc cgcgcgccag gtgctgattt gctccgccag cgggatcagt
    40621 tctttatccc acgggcgtag ctgttcgtaa cgagtcacct ggcgcagcgc cgcattgcgc
    40681 tgcttgagca atcgcttgag attgctccag gcggtgaaaa atccaggttc gttgtgaaag
    40741 catccccagt cgaggaatgc tcttctgtat ttggggccgc cgttgagtaa agtaaaccct
    40801 tctggcgtta tcaactgcat cggcatcagg tgcgccagtt ccgcgacttt atgcccgtcc
    40861 gtaccgtcaa tacggacttt gctgtcgccc tgtttgtctt tggttaagcc aatcgccgtc
    40921 tcgcgctctt cgccctgtaa tcgcccgtgg agaacaaatg cctcctgctc atggcgaatg
    40981 acgcgaccaa tctgcaaact gcgaaacgcc cgaccatggc cgagcgtata gatggcttcc
    41041 agcacgctgg ttttgccact gccgttggca cctaccagaa agttaaagcc gggagataaa
    41101 gcgagatccg cggtttcaat attgcggaaa tcgcggatca acaagcgggt gagggacatt
    41161 acagtctcat tggcatgaca acataggcag ccgactgtga tgcggcatct tcaatctgca
    41221 cgctggaaac cgaatcggtc agcatcatgc ggacgttttc gcatttcagc gcgttcagaa
    41281 catccagcac atagctgacg ttgaagccga tttccatctc cgcaccgcta taggtaacgt
    41341 cgaggatctc ttccgcttct tcctgttccg ggttgttggc agtaattttc agctggtttt
    41401 cgctgacata caggcgcaca ccgcggaatt tctcgttaga gagaatcgcc gcgcgagcaa
    41461 acgcctgctt gagcagatcg cagcctgctt ccagatgttt gtccgggttc ttcggcagaa
    41521 cgcgacggta atccgggaag cgaccatcca ccagtttcga ggtgaaaata aagtcgccaa
    41581 cgtgggcacg aatgttattg ctgccaatct gcacacgcag cagattgtcg ccgccgtcga
    41641 gcatacgcat cagttcaatc acgcctttac gcggtacgat caccgaatgg cttggcaaag
    41701 attgaccaat tggcattgaa cagaccgcca gacggtggcc gtcggttgcc acggtgcgca
    41761 gttcttcacc ttcggtttca aacagcatac cgtttaagta atagcgaaca tcctgatgcg
    41821 ccatcgaaaa ctgggttgct tcaatcagac gcttcatcgt cgcctgcggc agggtaaatt
    41881 cgacttcgct ctgccagtca tcgaggttcg ggaaatccgc cgctggcagg gtagacagcg
    41941 aaaaacggct acgcccggag cgcaccagca tccgttcacc ttccagctgc acggcaattt
    42001 ccgcgccttc aggcagacca cggcagatat caaagaattt gcgcgccgga acggtcgtcg
    42061 ctcctggctc gtgtggctga accagcgcaa cgcgtgccac catttccatc tcgagatcag
    42121 taccggtcag cgacaacgta ccgtcagcaa cctgtaacag cagattaccg agaatcggta
    42181 gcgtaggacg accacctaac ggaccgctca cctgttgtag cggttttaat aaatgctcac
    42241 gttctacggt aaatttcata ggtttacgat gacaatgttc tgattaaatt tgaaaaatct
    42301 tctttgatat cgtggctctc ttcacgcaac tgctcgatct tacggcaggc atgaagcacc
    42361 gtcgtgtggt cacgcccacc aaacgcatcg ccaatctctg gcagactgtg gttagtcagc
    42421 tctttcgcta gcgccatcgc catctggcgc ggacgcgcca ccgagcggga tcgacgcttg
    42481 gaaaggagat ccgcgacttt gatcttgtag tactccgcca ccgtcttctg aatattgtcg
    42541 atggtgacca gtttttcctg caatgccagc aagtcgcgca gcgcctcacg cacgaagtcg
    42601 atggtgatcg cccgtccggt aaagttggcg ttagcaataa cgcggttcag cgccccttcc
    42661 agctcacgta cgttagatcg tagacgcttg gcgataaaga acgccacttc acccggcaaa
    42721 cgaatgtcat tttcgtcggc ctttttcatc aggatcgcca cacgggtttc cagctctggc
    42781 ggttcgatcg ccacagtcag tccccaaccg aagcgggatt tcaaacgatc ctcaacgccg
    42841 ttgatctctt tcggatagcg atccgaggtg agaatgatct gttgattacc ttccagcagg
    42901 gcgttgaagg tgtggaaaaa ctcttcctga gatcgttctt tattagcaaa aaactgaata
    42961 tcgtcgatca gcagtgcatc tacggaacgg tagtagcgtt taaactcttc gatcgcgttg
    43021 ttttgcaggg ctttaaccat gtcctgaaca aagcgctcgg agtgcatata aaccactttg
    43081 gcattcggct tgcgcgccat aatgccgtta cccaccgcat gcagcaggtg agttttaccc
    43141 agacccgtgc cgccataaag gaacaacggg ttataggcac cgccagggtt atccgccacc
    43201 tggcaagccg ccgcgcgcgc cagttggtta gatttacctt caacgaagtt atcaaacgtg
    43261 tgtttgacgt ttacgttaga acgataggtc ggttctgccg gggccgggac gttatcccaa
    43321 cctgagcgcg tagaaggcgc agcacgttgc ggctgcgttt gcgccacctg tgcaggggcc
    43381 gcgacgttgc tcgtcactgc cgcttgtggc gtttgcgtca ccggtttggt gccgacttca
    43441 aaacgcagct gtggggcatc cgctccgcag aaactggtta gcagtccatt gatattatta
    43501 aggtacttgt cccgtaccca atcgaggaca aaacggtttg gcgcgtacag ggccagcgtg
    43561 ttatcgctca gttccgcctg caatgggcgt atccacatac tgaattctgt ggctggtaac
    43621 tcatcctgca atcgggcaag acactgctgc caaagcgaaa gtgacacggc ggactccact
    43681 cgaacaaaag tcgataatga ctaaggctga aacattcatg attgttgacg tacgtcgaaa
    43741 agaccctgct tgagggtgac gcacgaaccg ctgtctgcgg ttatatgccc gatcaagatc
    43801 ctgcaaaacg atcgggaccg cggatcatag cctaaactgc gcaagagatc ttctgtttct
    43861 cacagatttt ccctatttat ccacaggact ttccagaact cgctaagtgt aaacgatcct
    43921 gccgcgaggc gggcacgatt tacgccgcat attggaaaat ttaatgacca tagacaaaaa
    43981 ttggcttaat cgatctaata aagatccagg acgatccttg cgctttaccc atcagcccgt
    44041 ataatcctcc acccggcgcg ccatgctggt ttccactggt gtgaggtcgt acattttccc
    44101 tgcgaaaagg tgcggaaaag cgcggtaaat aaggaaagag aattgactcc ggtgtgtaca
    44161 attattacaa tccggcctct ttaatcaccc atggcttcgg tgtccatcgt ttcatttttc
    44221 ggcggatatc caataaagcc attgaattta ttcaagttta ggtagaaatc gccatgaaac
    44281 gcacttttca accgtctgta ctgaagcgca accgttctca cggcttccgt gctcgtatgg
    44341 ctactaaaaa tggtcgtcag gttctggcac gtcgtcgtgc taaaggccgc gctcgtctga
    44401 ccgtttctaa gtaataaagc taacccctga gtggttaagc tcgcatttcc cagggagtta
    44461 cgcttgttaa ctcccagtca attcacattc gtcttccagc agccacaacg ggctggcacg
    44521 ccgcaaatta ccattctcgg ccgcctgaat tcgctggggc atccccgtat cggtcttaca
    44581 gtcgccaaga aaaacgttcg acgcgcccat gaacgcaatc ggattaaacg cctgacgcgt
    44641 gaaagcttcc gtctgcgcca acatgaactc ccggctatgg atttcgtggt ggtggcgaaa
    44701 aaaggggttg ccgacctcga taaccgtgct ctctcggaag cgttggaaaa attatggcgc
    44761 cgccactgtc gcctggctcg cgggtcctga tagccctcat tcgggtctat caacgcctga
    44821 ttagtccgct actcgggccg cattgtcgtt tcactccaac ctgttcaagc tacggaattg
    44881 aggcattgcg caggtttgga gtgataaaag gcagttggtt gacggtgaaa cgcgtattaa
    44941 aatgccaccc tttacaccct ggtggtgacg atcccgtccc gcccggacca tttgatacca
    45001 gagaacacta acgatggatt cgcaacgcaa tcttttagtc atcgctttgc tgttcgtgtc
    45061 tttcatgatc tggcaagcct gggagcagga taaaaacccg caacctcagg cccaacagac
    45121 cacgcagaca acgaccaccg cagcgggtag cgccgccgac cagggcgtac cggccagtgg
    45181 ccaggggaaa ctgatctcgg ttaagaccga cgtgcttgat ctgaccatca acacccgtgg
    45241 tggtgatgtt gagcaagctc tgctgcctgc ttacccgaaa gagctgaact ctacccagcc
    45301 gttccagctg ttggaaactt caccgcagtt tatttatcag gcacagagcg gtctgaccgg
    45361 tcgtgatggc ccggataacc cggctaacgg cccgcgtccg ctgtataacg ttgaaaaaga
    45421 cgcttatgtg ctggctgaag gtcaaaacga actgcaggtg ccgatgacgt ataccgacgc
    45481 ggcaggcaac acgtttacca aaacgtttgt cctgaaacgt ggtgattacg ctgtcaacgt
    45541 caactacaac gtgcagaacg ctggcgagaa accgctggaa atctcctcgt ttggtcagtt
    45601 gaagcaatcc atcactctgc caccgcatct cgataccgga agcagcaact tcgcactgca
    45661 caccttccgt ggcgcggcgt actccacgcc tgacgagaag tatgagaaat acaagttcga
    45721 taccattgcc gataacgaaa acctgaacat ctcttcgaaa ggtggttggg tggcgatgct
    45781 gcaacagtat ttcgcgacgg cgtggatccc gcataacgac ggtaccaaca acttctatac
    45841 cgctaatctg ggtaacggca tcgtcgctat cggctataaa tctcagccgg tactggttca
    45901 gcctggtcag actggcgcga tgaacagcac cctgtgggtt ggcccggaaa tccaggacaa
    45961 aatggcagct gttgctccgc acctggatct gaccgttgat tacggttggt tgtggttcat
    46021 ctctcagccg ctgttcaaac tgctgaaatg gatccatagc tttgtgggta actggggctt
    46081 ctccattatc atcatcacct ttatcgttcg tggcatcatg tacccgctga ccaaagcgca
    46141 gtacacctcc atggcgaaga tgcgtatgct gcagccgaag attcaggcaa tgcgtgagcg
    46201 tctgggcgat gacaaacagc gtatcagtca ggaaatgatg gcgctgtaca aagctgagaa
    46261 ggttaacccg ctgggcggct gcttcccgct gctgatccag atgccaatct tcctggcgtt
    46321 gtactacatg ctgatgggtt ccgttgaact gcgtcaggca ccgtttgcac tgtggatcca
    46381 cgacctgtcg gcacaggacc cgtactacat cctgccgatc ctgatgggcg taacgatgtt
    46441 cttcattcag aagatgtcgc cgaccactgt gaccgacccg atgcagcaga agatcatgac
    46501 ctttatgccg gtcatcttca ccgtgttctt cctgtggttc ccgtcaggtc tggtactgta
    46561 ctatatcgtc agcaacctgg taaccattat tcagcagcag ctgatttacc gtggtctgga
    46621 aaaacgtggc ctgcatagcc gcgagaagaa aaaatcctga ttcggtgagt tttcgctaaa
    46681 ataagggcgg tcagttgacc gccttttttc ttttcgtagg gcggataagc accgcgcatc
    46741 cgccacacaa agcaacagga acatcatgag cgataatgac actatcgtag cccaggccac
    46801 gcctccggga cgtggcggcg ttggcatcct gcgcatctcc ggcttcaaag cccgtgaagt
    46861 tgccgaaacc gtgctgggta aactgcctaa gccgcgctac gccgattatc ttccgtttaa
    46921 agacgccgac ggcagcgtgc tcgatcaggg gattgcgcta tggttccctg gcccgaactc
    46981 gttcaccggc gaagatgtgc tggaactgca aggtcatggc ggtccggtga tcctcgacct
    47041 gctgttaaaa cgcattctga ccattcccgg cctgcggatt gctcgccctg gtgagttttc
    47101 cgaacgcgcg tttcttaacg ataaacttga cttagcccag gccgaggcga ttgccgatct
    47161 tatcgacgcc agttcggaac aggcggcccg ttcggcactt aactcgctgc aaggcgcatt
    47221 ctccgcacgg gttaatcatc tggtagaagc cctcacccac ttgcgcattt acgtcgaagc
    47281 ggcaattgat ttccctgatg aagagatcga tttcctctcc gacggcaaaa ttgaagccca
    47341 gcttaatgac gtgatggccg atctcgatgc agtgcgtgct gaagcacgtc agggtagttt
    47401 gttgcgcgaa gggatgaaag tagtgattgc cggacgtcct aatgccggta aatcgagcct
    47461 gttaaacgcg ctggcgggcc gtgaagcggc aatcgtaacc gatatcgccg gaaccacgcg
    47521 tgacgtgctg cgtgagcata tccacattga cggaatgccg ctgcatatca tcgacactgc
    47581 cgggctacgt gaggccagtg acgaagtgga acgtattggt atcgagcgcg cgtggcagga
    47641 aattgaacag gccgaccgcg tgctgtttat ggtcgatggc accacgacag acgccgttga
    47701 tccggcagag atctggccgg aatttatcgc ccgtctgccc gcgaaactgc cgatcaccgt
    47761 ggtacgcaat aaagccgata tcaccggcga aacgctggga atgagtgaag tgaacggtca
    47821 cgcgttaatt cgtctctcgg caaggactgg tgaaggcgtg gacgtgctgc gtaaccatct
    47881 caaacagagc atgggctttg acaccaatat ggaaggcggc ttcctggcgc gccgtcgcca
    47941 cttacaggcg ctggaacagg cagcggagca tttgcagcaa ggtaaagcgc aactgttggg
    48001 agcctgggca ggtgaactgc tggcggaaga gttgcgcctg gcacagcaga acttaagcga
    48061 aatcaccggg gaatttactt cagacgacct gctggggcgg attttctcca gcttctgtat
    48121 tggtaagtaa ccgcgcttac gaagccgtat tctgactgtc agatgcggct tcgcttcatt
    48181 gttagcactc ctgttatgcc tcaacccttt tttaaacatt aaaattctta cgtaatttat
    48241 aatctttaaa aaaagcattt aatactgctc cccgaacgat tgtgattcga ttcacattta
    48301 aacaatttca gaatagacaa a
//
LOCUS       DAJTSI010000012        47955 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000012 DAJTSI010000000
VERSION     DAJTSI010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.012"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="OO327_000622"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="OO327_000622"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR6017282.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="OO327_000623"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="OO327_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR6017283.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="OO327_000624"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="OO327_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR6017284.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="OO327_000625"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="OO327_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR6017285.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="OO327_000626"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="OO327_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR6017286.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="OO327_000627"
     CDS             5377..5835
                     /locus_tag="OO327_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR6017287.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="OO327_000628"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="OO327_000628"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR6017288.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="OO327_000629"
     tRNA            6887..6971
                     /locus_tag="OO327_000629"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="OO327_000630"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="OO327_000630"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="OO327_000631"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="OO327_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR6017289.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="OO327_000632"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="OO327_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR6017290.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="OO327_000633"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="OO327_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR6017291.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="OO327_000634"
     CDS             complement(10252..13995)
                     /locus_tag="OO327_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR6017292.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14388..15779)
                     /gene="narK"
                     /locus_tag="OO327_000635"
     CDS             complement(14388..15779)
                     /gene="narK"
                     /locus_tag="OO327_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR6017293.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16118..17914
                     /gene="narX"
                     /locus_tag="OO327_000636"
     CDS             16118..17914
                     /gene="narX"
                     /locus_tag="OO327_000636"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR6017294.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17907..18557
                     /gene="narL"
                     /locus_tag="OO327_000637"
     CDS             17907..18557
                     /gene="narL"
                     /locus_tag="OO327_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR6017295.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="OO327_000638"
     CDS             complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="OO327_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR6017296.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQYLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20137..20490
                     /gene="ychN"
                     /locus_tag="OO327_000639"
     CDS             20137..20490
                     /gene="ychN"
                     /locus_tag="OO327_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR6017297.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="OO327_000640"
     CDS             complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="OO327_000640"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR6017298.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="OO327_000641"
     CDS             complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="OO327_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR6017299.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21887..22987
                     /gene="chaA"
                     /locus_tag="OO327_000642"
     CDS             21887..22987
                     /gene="chaA"
                     /locus_tag="OO327_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR6017300.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23392..23499
                     /locus_tag="OO327_000643"
     CDS             23392..23499
                     /locus_tag="OO327_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6017301.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="OO327_000644"
     CDS             complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="OO327_000644"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR6017302.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24538..25347)
                     /locus_tag="OO327_000645"
     CDS             complement(24538..25347)
                     /locus_tag="OO327_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCR6017303.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVERCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(25351..25743)
                     /locus_tag="OO327_000646"
     CDS             complement(25351..25743)
                     /locus_tag="OO327_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR6017304.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="OO327_000647"
     CDS             complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="OO327_000647"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6017305.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLYALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="OO327_000648"
     CDS             complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="OO327_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR6017306.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="OO327_000649"
     CDS             complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="OO327_000649"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR6017307.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            29167..29790
                     /gene="lolB"
                     /locus_tag="OO327_000650"
     CDS             29167..29790
                     /gene="lolB"
                     /locus_tag="OO327_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR6017308.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            29790..30641
                     /gene="ispE"
                     /locus_tag="OO327_000651"
     CDS             29790..30641
                     /gene="ispE"
                     /locus_tag="OO327_000651"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR6017309.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            30792..31739
                     /gene="prs"
                     /locus_tag="OO327_000652"
     CDS             30792..31739
                     /gene="prs"
                     /locus_tag="OO327_000652"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR6017310.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            31864..33543
                     /gene="dauA"
                     /locus_tag="OO327_000653"
     CDS             31864..33543
                     /gene="dauA"
                     /locus_tag="OO327_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR6017311.1"
                     /translation="MNKIFSSHVMPFRALIDVCWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="OO327_000654"
     CDS             complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="OO327_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR6017312.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            34154..34738
                     /gene="pth"
                     /locus_tag="OO327_000655"
     CDS             34154..34738
                     /gene="pth"
                     /locus_tag="OO327_000655"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR6017313.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            34855..35946
                     /gene="ychF"
                     /locus_tag="OO327_000656"
     CDS             34855..35946
                     /gene="ychF"
                     /locus_tag="OO327_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR6017314.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            36790..39657
                     /locus_tag="OO327_000657"
     CDS             36790..39657
                     /locus_tag="OO327_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6017315.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="OO327_000658"
     CDS             complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="OO327_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCR6017316.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            41904..42974
                     /gene="dhaK"
                     /locus_tag="OO327_000659"
     CDS             41904..42974
                     /gene="dhaK"
                     /locus_tag="OO327_000659"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR6017317.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            42985..43617
                     /gene="dhaL"
                     /locus_tag="OO327_000660"
     CDS             42985..43617
                     /gene="dhaL"
                     /locus_tag="OO327_000660"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCR6017318.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            43628..45046
                     /gene="dhaM"
                     /locus_tag="OO327_000661"
     CDS             43628..45046
                     /gene="dhaM"
                     /locus_tag="OO327_000661"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR6017319.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            45366..47063
                     /gene="treA"
                     /locus_tag="OO327_000662"
     CDS             45366..47063
                     /gene="treA"
                     /locus_tag="OO327_000662"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR6017320.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKIADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(47142..47581)
                     /locus_tag="OO327_000663"
                     /pseudo
     CDS             complement(47142..47581)
                     /locus_tag="OO327_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        12043 a        12342 c        11741 g        11829 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgctgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaga ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggcg cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc acggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcca
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccgc ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccag gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aggcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcgttcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagcata acatgctgta aattaagacg ttttatacct atagttattt aaactattaa
    14221 gatttttatg caaatgggta ttaaggagta ttccccatgg gtaacatatt aatggctctt
    14281 aatgaaatcc ggcgaaataa gccatataac actaatatgt tgtaactaaa tgacgcataa
    14341 aaaaagcgcg gcatcatgcc gcgcaaagga taatcaaata ttaagattta ttttttagaa
    14401 tgccgaccat ataccgccca agtaatcact acgcaggcga tatagaaaat caaaaatact
    14461 ttcattgcac ctactggcga acccgttaat gccagcgagc taccaaacgc tttcgggata
    14521 aagaagccac caatcgcgcc aatcgcagag atgaaaccca gcgccgctgc cgtgccggtt
    14581 gccgcttcac gcatcgcacg ttcgtcagaa cccccttctg ctttcacgcg atccattgtc
    14641 agtttacgga agatcactga aatcatctgg aaagtggaac cactacccag cccagctgtc
    14701 aggaacagcg ccaggaagac cgcgaagaac gccatgaagc ttccgccctg cccgtcagtc
    14761 ggtaaggtca ggaacagcag gccgctgaaa atcgccatca gaataaagtt caccagcgtg
    14821 acacgagttc cgcccagacg gtcagataat gcaccacctg cagaacgcgc cagcgcacca
    14881 ataaacggcc cgaagaaagc gtattgcaga atctgaacat ccgggaactg cgtttttgac
    14941 agcatcgcaa agcccgcgga gaagccgatg aaggagccga aggttgccag atacagcagg
    15001 ctcataatcc acagatgacc ccgtttgagt accggcaact gctccttgat ggaggctttc
    15061 gaggtagcaa gatcgttcat gccaaaccac gccgcaatgg tgaagatggc aaggaacggc
    15121 acccatatcc aggacgcatt cgccagatac agctcagtcc catccggctg tttgacgccc
    15181 tggctaccaa atactgcgaa aatcgacagt gataccacca gcggagcaac caactgcatg
    15241 acgctgacgc ccatgtttcc cagaccacca ttcagaccca gcgcgccacc ctgcttctgt
    15301 ttcggaaaga agaagctgat gtttgccata ctggatgcga agttcgcgcc agcaaagccg
    15361 catagcagag agatgatgat gaagacgcta taaggcgtgg aggtatcctg cacggcaaaa
    15421 cccagccaga cgcaaggaat aatcagaata ccggtgctga acgccgtcca gcgacgacca
    15481 ccgaagatag gaaccataaa ggagtatgga acacgtaata acgcgccaga aaccgaaggc
    15541 agcgcagtca acataaatag ctgatcggtc gtaaaattaa aaccgacttt cggtaagttc
    15601 accgcaacag cgctgaacaa catccatacg caaaacgcca gcagcagaca gggaacggaa
    15661 atccacaggt tgcggctggc aatacgttga ccgcgttgtt gccagaacgc aggatcttcc
    15721 ggtcgccaat ctgttatgac agctccagta gccctttcgg aggcagatga gtgactcata
    15781 gacacctctg atactcgttt cgatgtctgc caccttagtg tctggagcta aaggcaattt
    15841 gatgtaaatc aaacgataag ataactttat ctatcattga tatttatcat tacccatagt
    15901 gagtacagtg atttcataaa aattatgaga tttttcacgg tgctgtagaa tccctaccct
    15961 taccgatgta aagcgactaa ccacacggca aataaggagt aactctttcc ggctatgggt
    16021 atacttcagc caatagccga gaatactgcc attccagaat atatcgtcac attcattaag
    16081 gttattgctc atttaaagcc tgaaggaagg ggtttacatg cttaaacgtt gtctctctcc
    16141 gctcaccctg gttaatcagg ttgcgcttat tgtgttgctt tctactgcta ttggactggc
    16201 agggatggcg gtttctggct ggctggtgca aggcgttcag ggcagcgccc atgcgatcaa
    16261 caaagcggga tcgctgcgca tgcaaagtta ccgtctgttg gcggcagtgc cattaagcga
    16321 gaaagacaag cccttaatta aagagatgga acaaacggca tttagcgccg agttgactcg
    16381 agcagcagaa cgagacggac aactggcgca attacagggc ttacaagatt actggcgtaa
    16441 tgaactgatc cctgcgctga tgcgtgcaca aaaccgagaa acggtgtcag cggatgtcag
    16501 ccagtttgtt gccgggcttg atcagctggt atctggtttt gaccgcacca cggaaatgcg
    16561 tattgagaca gtggtactgg tccatcgggt aatggcggta tttatggcac ttttactggt
    16621 gttcactatt atctggttgc gggcgcgact gctacaaccg tggcggcaac tgctggcaat
    16681 ggcgagtgcc gtcagccatc gcgattttac ccaacgcgca aacatcagcg ggcgcaacga
    16741 aatggcgatg cttggaactg cgttgaacaa tatgtctgca gaactggccg aaagttatgc
    16801 cgtacttgag cagcgggttc aggagaaaac cgccgggctg gagcataaaa atcagatcct
    16861 ctctttttta tggcaggcta accgccgttt gcattcccgc gccccgctgt gtgaacgcct
    16921 gtcaccggta ctcaacggct tacagaattt aaccctgcta cgtgatatcg aactgcgggt
    16981 gtatgacact gatgatgaag agaatcatca ggagtttacc tgccagccag atatgacttg
    17041 tgatgataaa ggctgccagc tctgcccgcg cggcgtatta cccgttggtg atcgcggcac
    17101 gaccctgaag tggcggctgg ctgactctca tacgcagtac ggtattttgc tggcgaccct
    17161 gccgcagggg cgtcatctta gccatgacca acaacaactg gtggataccc tggttgaaca
    17221 acttaccgcc acgctggcgc tggatcgcca tcaggaacgt cagcaacagt tgatcgtgat
    17281 ggaagagcgt gccaccattg cgcgcgaact gcatgattct attgcccaat ctctctcttg
    17341 catgaagatg caggtgagtt gtttacagat gcagggcgat gcgctgccag aaagcagccg
    17401 cgaactgtta agtcagatcc gtaacgaact gaatgcatcc tgggcgcagt tgcgtgaatt
    17461 gctcaccaca ttccgcttgc agctcaccga gcctggatta cgtccggcgc tggaagcgag
    17521 ttgcgaagag tacagcgcca aatttggctt cccggtgaag ctggattatc aattgccgcc
    17581 tcgtctggtg ccttcacatc aggcaatcca cttgttgcaa attgcccgtg aggcattaag
    17641 taacgccctc aaacattcgc aagcgagtga ggtcgtggtg acggtggcgc aaaacgataa
    17701 tcaggtcaaa ctgaccgtcc aggataacgg ctgtggcgtg cctgaaaatg ccatccgcag
    17761 caatcactac ggcatgataa taatgcgcga ccgtgcgcaa agtttacgag gcgattgccg
    17821 cgtccgccgt cgtgaatcag gtggcaccga agtggtggtc acctttattc ccgaaaaaac
    17881 tttcacagac gtccaaggag atacccatga gtaatcagga accggctact atcctgctga
    17941 ttgacgatca cccgatgctg cgaactggcg taaaacagct tatcagtatg gcaccagata
    18001 tcaccgtggt tggcgaagcg agtaatggcg aacagggtat tgaactggcg gaatctcttg
    18061 atcccgatct gatcctgtta gatctcaata tgcccggcat gaacggtctg gaaacgctgg
    18121 ataaactgcg cgaaaagtcc ctctcagggc gcattgtggt attcagcgtc tctaaccatg
    18181 aagaagatgt ggtcaccgca ctgaaacgcg gcgcggatgg ctatctgtta aaagatatgg
    18241 aaccggaaga tctgctgaaa gcattgcatc aggcagctgc tggcgaaatg gtattaagcg
    18301 aagcattaac gcctgttctg gccgccagct tgcgcgctaa ccgtgccact actgagcgcg
    18361 atgttaacca gttaacccca cgcgagcgcg atattctcaa gctgattgcc cagggtttgc
    18421 cgaacaagat gattgcccgc cgcctggata tcaccgaaag cacagtaaaa gtgcacgtca
    18481 agcacatgct gaagaaaatg aagctcaagt ctcgcgtgga agcagcggta tgggtgcatc
    18541 aggagcgcat tttctgatta cggttcccag cgcagttcgt cgtttgacaa tgcatcgaac
    18601 ggttcgacaa gcgttagcgt gatctcattg gaggagacac gctgcccctg gttatcttcc
    18661 accaccacag acaatcgcca gtgattgctt gccccttccc cgttctgcca gtcaggcatg
    18721 atcagcgtcc agccctccgc gctgttggct tgtgcgcccg gcgtcaaact taatatctgc
    18781 gtatcgccct gccaaatcag ttgccgaata ccgtaacgac tgcggatttg taatttcagc
    18841 ggcactgttt cgccaggttt tagatcccac ggcggtgtcg ccagaaacac cgttaacgtt
    18901 tttcgctgtc ggtactcaag agtcggtaga ttatttcgct gcggattgtc atagcgacta
    18961 ccacgtaacg actgactttc ggcaacctcg cccgcagaaa gttgtttttt gagcggtaca
    19021 ccaaagcggt aattaagatt cagcccgagg ttattttgat tttcgccact ttccccctgt
    19081 ttatgctggg ccgtcacagt gactaatggc acaggggtgt aatttaatcc cagactcaac
    19141 gcgacgggat tgtgataacc cgtaccagag ttaaacaaat caacacgatc accaaaatac
    19201 tgttctaggc tgacactggt attgaggtat tgatagaacg gcatgcgcat ccgagctgtc
    19261 aggtcgtacc cgcgcgccat ccgttgttcc tgcgtggctg tctgttcatg ccatgcagca
    19321 aacggctgat aaaagtttgc cgataatcgc aaatattcgc cccacgcttc ggcaccaaag
    19381 cccgctcgct gaagattttc gtcctgcaag ttgtcataaa aagtgttata acccaccagc
    19441 cagttgccgc gcgcccagcg ttgcccaacg cccacattgc tcaccaaccc attatcctgc
    19501 tgagtaagac caagctggct ccaggtgaga taacgatcat tatcttgtaa cggcacaaac
    19561 cagcttccac gactgccggt gaaatgtcct tcgttatcca ctttgacgtc aacactggca
    19621 tttccccacg gtgatagcca ggactctacg tgctgattaa cctgttgact aagcgcgtcg
    19681 cggacttttc ccaatgcgaa agcttttgcc tgctcgccag tatccagccc gttatcattc
    19741 atactggttt cgccgaaatc tttcacaatt tcagcaaagt gtttttcccc atcatgattt
    19801 tcgggagcca tgcctaaatc cggcagacca tcattgttat tatcaaaggg atttgccgct
    19861 ttttgctcga aggtagattg tgcgttagct gtaccgcctg ctacaagcaa gagtaaataa
    19921 aacgggataa tgcgaggaac gaaacggctc aaaacgtcgg taacttccgg taataatgaa
    19981 aaaccatagc cttaaagaat aacgattact ggcaactttt gtcgtatttt ctcgcgcttt
    20041 caggaaaatc cttaaaaatg tgaccgattg gtcagacgct gcagaagcag taagatagaa
    20101 gatgagtgtc taattccctg aatacaggag taaataatgc aaaaaatagt gatcgttgcc
    20161 aatggcgcac cttacgggag cgaatccttg tttaacagct tgcggctggc cattgcgtta
    20221 cgagagcagg agagcaatct ggatctgcgt ctgttcctga tgtctgatgc ggtcacagcc
    20281 gggttgcgcg ggcaaaaacc aggggaaggc tacaacattc agcaaatgct ggagatcctt
    20341 accgctcaga atgtaccggt gaaattgtgc aaaacctgta ccgacgggcg cgggattagc
    20401 acacttcctc tgattgatgg ggtggaaatc ggtactcttg tggaactggc gcaatggacg
    20461 ctgtcagccg ataaagtgct cacattttaa tgtttaaggg ggcgtctgac gccccactat
    20521 gagggaagtt tactcaggcg aatcccggcc ttaacacacc atccggataa ttctccgcca
    20581 gtagtttttt taccgatacc agcaaatcat tcagcccatc gtcctgcatt cccagtttga
    20641 taagctcctg ttccagtgaa aacaggtatt gtgcattggt tcccagcgga ccgctcgccg
    20701 ccgcaatcaa cggcgcgatg acctgagcgc gagtgtcaga ttcatattct ggatgtcgcg
    20761 ggtccataat aaacacaatg gcgtttactg tgcgtccatc atcaagatca agctgacacc
    20821 aggttggcag ataacagccg gtaatcatct cgcgcttcca caacagggtt agttcctgct
    20881 ccagcgtctc ttctggtagt cgataggcga cgcctgtggt gcgtccgccc tctttcagtg
    20941 caagcatccg tcccggctgg tgcgcagtcc cgcgcccggc ggtcaggcgc aggcaaaacg
    21001 cgcgatgcca tccaaccagt gtaccggtgc atgattcggt aaactccagt gccggattcc
    21061 acatcaacga tccataaccg aagatccaca ccggtccctc atcaggtcga caagccagcg
    21121 tcgccgccag cgaagccgcc cgttgttctg ctgaccataa gagtgattct tcaatggcac
    21181 caaatgccgt tttacaatcg gcattcatca agaaatcacg cgttatcacc ttacacctcc
    21241 gccactgcat acttccctgc gacaactttt tggtgtcttc cggacatttt taccgctcat
    21301 tttgaaacca ctaacggaac aatatgcaat ccacggacca cttgcaaggc aacaccgcac
    21361 gaatagcttt aactaagcta ccggttttac gattttttat gccatttatc atcatcccct
    21421 ttggcatatt catgcttcac agcagcccag gccactttat gcgcggtttc ttcgcgactg
    21481 gcgtcatcac gccgatcttc tttatcttta tattgatccc atgcgctgtt gaacgcttct
    21541 ttatagatat cctgggcatg agacggtaga acgtgcttta cgctttccgg cagatcgctt
    21601 ttcgttttat acggcatcgt tgactccttt tacaaagttg aacattaagt gtggtcaaca
    21661 atatctactc ttgccaaaac ataagaattt tcatgtcaga ctgttcttaa gaaacgctaa
    21721 aacaaacatt gtctaaacgt aatacttatt tttatgcccc aatgagtaaa aaatcgacag
    21781 cggggtaaaa attcgtaaaa tgttaccgat atccagacac tttctgttat ttttcctacc
    21841 tattttttac attttcgata aattcatctg caaagggata accataatgt caaatgctca
    21901 agaggcggta aaaacccgcc acaaggagac ttcgcttatt ttcccggttc tggtgctggt
    21961 agtgctgttc ctgtggggaa gtagccagac actaccagtg gtcattgcca tcaatcttct
    22021 tgcgcttatt ggtattttaa gtagcgcctt tagtgttgtc cgtcatgcgg acgtattagc
    22081 ccatcgcctg ggagaacctt acggttcgct tattcttagc ctttcagtgg ttattcttga
    22141 agtcagtttg atttcagctt taatggcaac cggcgacgcc gcgccaacgc taatgcgtga
    22201 tacgctctat tcaatcatta tgattgttac cggtgggctg gttggctttt cattattgtt
    22261 gggcggtcgt aagtttgcca cccaatatat gaatctgttt ggtatcaagc agtatttaat
    22321 tgcgctgttc cccctggcga taatcgtact ggtatttcca atggctctgc ctgcggcgaa
    22381 tttttcaacc ggtcaggcgt tactggtagc attaatttct gcggcaatgt atggcgtatt
    22441 tttgctgatc caggccaaaa cgcatcaaag tttgtttgtc tacgagcacg aagatgacag
    22501 tgatgacgac gacccgcacc acggtaaaac gtctgcccat agcagcctgt ggcatgctat
    22561 ctggttgatt atccatctga ttgccgttat tgcagtcacc aaaatgaacg ccagctcgct
    22621 ggagacattg ctcgacagca tgaatgcccc tgttgccttt actggcttcc tggtggcact
    22681 gttgattctg tcgccggaag gtttaggtgc gttaaaagca gtgttgaaca accaggttca
    22741 gcgcgcgatg aatctgttct ttggttcagt gttagcaaca atttcgctta ccgtacctgt
    22801 cgtcacgcta attgccttta tgacgggtaa cgaattgcag tttgcacttg gtgcgccaga
    22861 aatggtggtg atggtggcct ctttagtgct gtgccatatc tccttctcca ccggacgtac
    22921 taacgtgctc aatggcgcag cgcatctggc actgtttgcc gcctatttga tgacgatatt
    22981 tgcctgacag ttatatctcc tccgatgacg gaaatcggga gagataagat aaattcctgc
    23041 ttattgtatt tcttcatact acctggacag ctcgttaatc agaattgcga gcctgtacca
    23101 caacctgcac gaaaccactg caacagctgt tgaaaagccg gaatgtactc tttattgcaa
    23161 acatttcagg gatgaagtga agatgaattg agacgtagta tccacaccaa gccggaatta
    23221 tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggaaagag
    23281 aaaacccccg cacgttgaga ggtaaaacct gacaacaaaa cgggggctaa tcttgaaacc
    23341 agacacttca agaatagccg caaaatgttg tcattacaac aaggcggcta tatgacgctc
    23401 gcgcagtttg ccatgacttt ctggcacgac ctggcagcac cgatcctggc ggggattatt
    23461 gccgcagcga ttgtcagctg gtggcgtaac cggaagtaag gtgaaaacgg gcgtcaggct
    23521 gccgtgatgg caatatgcgc ccgaaaccgg ccgcaggggt aacagcctgc ggcctttttt
    23581 gtatctggca cccatgaaaa aagtcttaac gcagaacgct aagactttat ttttcaagca
    23641 aaaaagatta cttgctggta tccagttctt cgaaaccttt caccagatca tcaatcgctt
    23701 tcatctgctt gaggaacggt tccagtttag ccagcggcag cgcggatgga ccatcacatt
    23761 tcgcatgttc cggatccgga tgcgcttcaa taaacagccc cgccagacct accgccatac
    23821 cggctcgtgc cagctcagcc acctgagcac gacgaccacc ggaagctgcg ccaaacggat
    23881 cgcggcattg cagtgcgtgg gtcacgtcga aaatcaccgg cgagttacca gacactttct
    23941 tcataatgct gaagcccagc atatcgacaa ccaggttgtc atagccgaag ttagcaccac
    24001 gatcgcaaag aatcactttt tcgttgccgc cttctttgaa tttatcaacg atattaccca
    24061 tctgtcccgg gctgacaaac tgtggtttct tgacgttaat taccgcaccg gttttcgcca
    24121 tggcttcaac caggtcagtc tggcgagcaa gaaacgccgg caactgaatc acatccacga
    24181 catcagcaac gggctgtgcc tgacttggtt cgtgaacgtc ggtgataatt ttcacgccaa
    24241 aagtctgctt caactcctgg aagattttca tcccttcttc caggcccggt ccacgataag
    24301 agtggatgga ggagcggttg gctttgtcaa aagaggcttt gaacacgtaa gggataccca
    24361 gtttctgggt cacagttacg tagtgctcgc aaatgcgcat cgccagatcg cgagattcca
    24421 gcacgttcat accgccaaac agtacgaacg gcaggtcatt tgctacgttg atgtcgccaa
    24481 tgctaaccac tttttgtttc ataggatcgc cttaatcttg agtaaaatgt cgattaatta
    24541 atgcagcaca atatgtttat gcgcgatgtt atttatttgc gcacggatca tttcgctgat
    24601 cgggtcttcc ggacaccgtt caacgaaata acttaaatcg ttcaacgcaa cgtgttcgca
    24661 atccagttgc gcataaatca acccacgatc gcgaatttca tagggatctt cagggttgaa
    24721 ttgtaataaa gcttcgctgg tgcgtaacgc cagctccatc tgattttctt ccatcaacga
    24781 ggctttgagt gtatccagca atttgcggat tacctcaatg ttatcagctt catcaaggtc
    24841 ttcataaaac agttccgccg acgggctgat atttcccttt aaccatccgt ccagcatatg
    24901 ttcgcttaac gattcaccgt taaaaggatt aatcagccaa atttcgccat ccggacattc
    24961 aatgcgcaat atcagctgcg tagggaaaat caccggcagc agcggcaaat cgagacgatt
    25021 cgcgacccat aataaaaccg cacctaatga taccgcactg ccctgtcgat tctttaacac
    25081 ctggtccagc cataatgcat cggaaagacg ataaacgccg cgtgaggctt taaatcccca
    25141 gtcgccgtaa aacagcgcga tcagtttttc cagctgctct tctaaaggca gaagctggct
    25201 gatttcttcc ttcgccagac taacgagacg ctccagttcg tcgtaaacat cttgcgaggg
    25261 aaaatcgcgg cggattgctt cgcaagccag gatcatgcct tcgcacaatg gcgctttatt
    25321 aaattcgaaa tcagctaacg atctcatgac ttaccccagt aacggtactt ttgtggtggc
    25381 gagtttaatg atgatgtaca gcaccaccaa cgccagcggg aaggcaataa tgcgcgcctg
    25441 ctgactgtgc atacgacgat aatcgagtgc aataaaaccc aaaacgatat aaatgataac
    25501 tccaaacagc ttttcagtca gccactgtgc ctgcccggaa aatggcagga tgtgcgcttt
    25561 agccatcaac gcaatgccgc ttaacaataa caccgtatcg acaaccggcg gcacaatgcg
    25621 cgtccagcga gcaaatgcct gtggatgctt ctgataacgt agccagaaac gtaaggttaa
    25681 tagcccaaca gaaagcgcga tactaataag atgaacacta agcagtgtag aaaaacttgt
    25741 cattgataat agcggccgag cgttacgcgc tcgttatcac catagtcacg gcaggtttcg
    25801 acgtcatgat accccgcgag gataaatgct tgtcgcaccg cttcgccctg ctgccagcca
    25861 tgttccagaa gcagaaagcc gccggatacc agcgcgttac gcgactgttc gatgatatgc
    25921 acgatgtctg ccattccact gtctgccgca accagcgcag tgagcggctc aaagcggaca
    25981 tcgccttgtt gaagatgtgg gtcctgctcg tcaatatacg gcggattgct gacaatcatc
    26041 gcaaactgct gcccggctag cgcgctaaac cagtcgcttt gcagaatgtg gatatttttg
    26101 atcgccagat gctgggcatt acgttgtgcc agggagacag catcaggcat acgatctaca
    26161 gcgataattt cgcagtccgg gcgctcgcta gccagcgcaa gcgcaatcgc cccggtaccc
    26221 gtcccgagat cgagaatacg gcaaggttgt tcaggcaacc gcgccagtgc ctgctccacc
    26281 agacactccg tatccgggcg cggaattaag gtcgctggcg aaacaaataa cggcaacgac
    26341 cagaattctc gcaccccggt taaatgagca atgggttcac catcgcgacg acgtgtcagt
    26401 agcgcataaa gttgctgaca ttgttcgtca gtcagctgcg tttcaccaaa ggcgagaata
    26461 aaagtacgcc ctctgccggt aacatgctcc agcaggattt cagcatcacg ccgcgggctt
    26521 tcgctcgcct gaagttggct tattgcttca cgtaaccagt gttgatattc cattattcct
    26581 gctcggacaa cgccgccagt tggtcggcct gatgttcctg gataatcggt tcaatcagca
    26641 tatccagctt accttccatc acttcatcca ggcggtagag cgtcaggttg atgcggtgat
    26701 cggtaacgcg cccctgcggg aaattgtaag tacggttacg gtcgctgcga tcgccactcc
    26761 ccagcaggtt acgacgggta gacgcttcgg cctgttggcg ttttgccatt tcagcagcgt
    26821 ggatgcgagc accgagcact gaaagcgcct tcgctttgtt tttatgttgt gaacgttcgt
    26881 cctgacattc aacaacaatc ccggtcggca agtgagtaat acgaattgcc gaatcggtgg
    26941 tgttaacgtg ctgaccaccc gcccctgacg agcggaaagt atcaatgcgt aaatctgctg
    27001 ggttgatgtc cggcagttct gcgtcaggca gttctggcat taccgcaacg gtacaagcag
    27061 aagtatgaat acgaccctgc gattccgtag caggaacacg ttgcacgcga tgaccgccgg
    27121 attcaaattt cagacgacca tacacaccat caccgctaat tttggcgatg atctctttat
    27181 aaccaccatg ttcaccctcg ctggcgctca tgatttctac ccgccagcgg cgggcttcgg
    27241 cgtaacggct gtacatacgg aacagatcgc ctgcaaacag cgctgcttcg tcgccgccgg
    27301 ttccggcacg gacttcgagg aaggcgttac gttcgtcatc aggatctttt ggtagtaata
    27361 gaacctgtaa ttgctgttcc agttgctcgc ttttttcttt agcttcgcgc agttcatcct
    27421 gcgccatctc acgcatttca ggatcgtcga gcatcatctg tgcggtttcg atatcttcct
    27481 gaacctgttg ccagtcggta aaacagcgcg aaacatcact taactgcgca tattcgcgtg
    27541 ataatgcgcg aaaacgttcc tggtcggcga tagtttgcgc gtcacccagc aacgcctgaa
    27601 cttcttcatg gcgttcatgc agggcttcca gtttggcaac gatagaaggc ttcataggcg
    27661 taaatgcacc ctgtaaaaaa agaaaatgat gtactgctac tccagcccga ggctgtcgcg
    27721 cagaatattc aggcgttcgt tatccccgtc acgggcggcc tgttgaagtg atttcgttgg
    27781 cgcatggatc aagcggttag tcagtttcca tgccagatcc tgcataatgg cttgcgcgtc
    27841 gccgccctgc tcaagggccg ctaacgcttt ggcggttaac tcatcgcgaa cgtgctctgc
    27901 ctggctgcga tactcgcgaa tggtttcgct ggcgctttgt gctcgcagcc acgccataaa
    27961 ttcgctggtt tcctgagcga caatagtttc cgcctcaacc gctgcggctt tacgctgcgc
    28021 caggttgtgc gaaatgatgc tttgcagatc gtccacacta taaagataag cattcgccag
    28081 tttgccaact tccggctcaa catcgcgcgg aacggcaata tccaccaaca gcattggttg
    28141 gttacgacga ctttttaatg cgcgctccac catgcctttc ccgataatcg gtaacgggct
    28201 ggcggtggaa ctgatgatga tatcggcttc gcgcagacgt tcgtcgatct cactcagggc
    28261 aatcacttcc gcgccaactt catctgccag aatttgggca cgttcgcgag tgcggttggc
    28321 gataatcatc ttctgtactt tatgttcgcg cagatgacgc gctaccagct cgatggtttc
    28381 gcccgcgcct accagcaaca ctgtgaccgt agagagcgat tcaaagatct gccgcgccag
    28441 cgtacaagcc gcaaaagcga cagacacagc actggcaccg atatctgttt cagtgcgaac
    28501 gcgtttcgct acagagaaag atttctggaa catgcgttcc agttcgctgg ccttcatatg
    28561 acctttttgc gaatcgacaa acgctttttt aacctgaccg aggatctgcg gctccccaag
    28621 aaccagtgaa tccaggccgc tggcaacacg cattaaatgg ctaaccgcgt cgttatcctg
    28681 atgccagtag aggcttttac gcagatcttc ttcattaaga ttgtgataat cgcaaagcca
    28741 gcggattaac gcctcttgca ggttatcctg ctcttcaacg ctaagataaa gttccgtgcg
    28801 gttgcacgtc gacagcacca cgccgccctg caccatcggc tgcgcaagca ggctgtcaag
    28861 cgcctgatcg agcttatccg gcgaaaacga tacacgttct cgcagcgata caggtgccgt
    28921 tttatggttg ataccgagtg ctaaaagggt catgtctgcg ggaaataata ccaacgttga
    28981 tagggttagt ctgcttgcat catacaggat gcgtaagatc aataaaagag agcgccccct
    29041 tttggagtaa ttgccgaaag ccgttagatt ctggcaatta agacaacttg aacatagacg
    29101 atagcggacg gtaacgctag cattaagggt tataactgca acgtatctca aggacttgtc
    29161 atcactatgc ccctgcccga ttttcgtctt atccgcctgc taccgctggc tgctcttgtg
    29221 ctcactgcct gttccgttac cacgcccaaa ggtcctggca aaagcccgga ttcgccacaa
    29281 tggcgtcagc atcagcaaga cgtgcgcaat cttaatcagt atcagactcg cggcgcgttc
    29341 gcttatattt ctgaccaaca aaaagtgtac gcccgctttt tctggcagca aaccggccag
    29401 gatcgctacc gtctgctgct cactaaccca ttgggcagca cggaactgga gctgaatgct
    29461 caaccgggta acgtgcagtt agtcgacaat aaaggtcagc gttataccgc cgatgacgcc
    29521 gaagagatga ttggcaaatt gaccggaatg ccaattccgc tcaacagctt gcgccagtgg
    29581 attttaggtt taccgggtga tgcaaccgac tacaaactgg acgaccagta ccgcctgagc
    29641 gaaattacct acagccagaa tggcaaaaac tggaaggttg tttatggtgg ttatgacacc
    29701 aaaacgcaac ctgcgatgcc agccaatatg gaactcaccg acggtggtca acgcatcaag
    29761 ttaaaaatgg ataactggat agtgaaataa tgcggacaca gtggccctct ccggcaaaac
    29821 ttaatctgtt tttatacatt accggtcagc gtgcggatgg ttaccacacg ctgcaaacgc
    29881 tgtttcagtt tcttgattac ggcgacacca tcagcattga gcttcgtgac gatggggata
    29941 ttcgtctgtt aacgcccgtt gaaggcgtgg aacatgaaga taacctgatc gttcgcgcag
    30001 cgcggttgtt gatgaaaact gcggcagaca gcgggcgtct tccgacggga agcggtgcga
    30061 atatcagcat tgacaagcgt ttgccgatgg gcggcggtct cggcggtggt tcatccaatg
    30121 ccgcgacggt cctggtggca ttaaatcatc tctggcaatg cgggctaagc atggatgagc
    30181 tggcggaaat ggggctgacg ctgggcgcag atgttcctgt ctttgttcgg gggcatgccg
    30241 cgtttgccga aggcgttggt gaaatactaa cgccggtgga tccgccagag aagtggtatc
    30301 tggtggcgca ccctggtgta agtattccga ctccggtgat ttttaaagat cctgaactcc
    30361 cgcgcaatac gccaaaaagg tcaatagaaa cgttgctaaa atgtgaattc agcaatgatt
    30421 gcgaggttat cgcaagaaaa cgttttcgcg aggttgatgc ggtgctttcc tggctgttag
    30481 aatacgcccc gtcacgcctg actgggacag gggcctgtgt ctttgctgaa tttgatacag
    30541 agtctgaagc ccgccaggtg ctagagcaag ccccggaatg gctcaatggc tttgtggcga
    30601 aaggcgttaa tctttcccca ttgcacagag ccatgcttta agccgggcaa gctgagtttc
    30661 ggtgacaacg tcaccttgtt ccagacgttg catcgcgctc tttaatacac cgcctggaaa
    30721 ggatcatgcc tggcccgcac agttttcggc agattctttc caccaatgga cgcatgcctg
    30781 aggttcttct cgtgcctgat atgaagcttt ttgctggtaa cgccaccccg gtactagcac
    30841 aacgtattgc caaccgcctg tacacttcac tcggcgacgc cgctgtaggt cgctttagcg
    30901 acggcgaagt cagcgtacaa attaacgaaa atgtacgcgg tggtgatatt ttcatcatcc
    30961 agtccacttg tgcccctact aacgacaacc tgatggaatt agtcgttatg gttgatgccc
    31021 tgcgtcgtgc ttccgcaggt cgtatcaccg ctgttatccc ctactttggc tatgcgcgcc
    31081 aggaccgtcg cgtccgttcc gctcgtgtac caatcactgc gaaagtggtt gcagacttcc
    31141 tctccagcgt cggtgttgac cgtgtgctga cagtggatct gcacgctgaa cagattcagg
    31201 gtttcttcga cgttccggtt gataacgtat ttggtagccc gatcctgctg gaagacatgc
    31261 tgcagctgaa tctggataac ccaattgtgg tttctccgga catcggcggc gttgtgcgtg
    31321 cccgcgctat cgctaagctg ctgaacgata ccgatatggc aatcatcgac aaacgtcgtc
    31381 cgcgtgcgaa cgtttcccag gtgatgcata tcatcggtga cgttgcaggt cgtgactgcg
    31441 tactggtcga tgatatgatc gacactggcg gtacgctgtg taaagctgct gaagcgctga
    31501 aagaacgtgg tgctaaacgt gtatttgcgt acgcgactca cccgatcttc tctggcaacg
    31561 cggcgaacaa cctgcgtaac tctgtaattg atgaagtcgt tgtctgcgat accattccgc
    31621 tgagcgatga aatcaaatca ctgccgaacg tgcgtactct gaccctgtca ggtatgctgg
    31681 ccgaagcgat tcgtcgtatc agcaacgaag aatcgatctc tgccatgttc gaacactaat
    31741 cgaacccggc tcaaagaccc gctgcggcgg gtttttttgt ctgtaatatc catttgtatg
    31801 acctatgcct ccttcacctg ccatttagtt gacagatgat gcgctcacgg atgaaacatt
    31861 attgtgaaca aaatattttc ctcacatgtg atgcctttcc gcgctctgat cgacgtttgc
    31921 tggaaagaaa aatatactgc cgcacggttt acccgtgacc tgattgccgg gataaccgtc
    31981 gggattattg ctatcccgct ggcgatggcg ttggctattg gtagtggtgt ggccccccag
    32041 tacggtttat ataccgcagc tgttgcgggg attgtcattg ctctaacggg tgggtcacgc
    32101 tttagcgttt ccggtccgac tgcggcattt gtggtaattc tctatcccgt ttcgcaacag
    32161 tttggactgg caggactgct ggttgcgacc ttgttgtcgg gaatcttttt gattctgatg
    32221 gggctggcac gctttggtcg tctgattgag tatattccgg tttccgtcac cttaggtttc
    32281 acctcgggta tcgggatcac catcggtacc atgcagatta aagattttct cggtctgcaa
    32341 atggcccatg tcccggaaca ttatctacaa aaagtcggcg cattatttat ggcgctgccg
    32401 accattaatg tgggtgatgc tgccattggc attgtgacgc ttggcattct ggttttctgg
    32461 ccgcgtctgg gcattcgttt acccggtcac cttccggcct tgctggctgg ttgcgcggtg
    32521 atggggattg ttaacctgct cggcggacat gttgctacca tcggttcgca attccactac
    32581 gtcctggccg atggttctca gggtaacggt attccgcaac tgctgccgca actggtgctg
    32641 ccgtgggatc tgcctaattc agaattcacg ctaacctggg attctattcg caccctgctg
    32701 cctgcggcat tctcaatggc aatgctcggg gcgatcgaat ctctgctctg cgctgtggta
    32761 ctggatggta tgaccgggac taagcacaag gcgaatagtg aactggtcgg tcaggggctg
    32821 gggaatatca tcgctccatt ctttggtggt attaccgcta ctgctgccat cgcgcgttct
    32881 gccgctaacg tccgtgccgg ggcaacttcc cctatctcgg cggtgatcca ctctattctg
    32941 gttattctcg ccctgctggt actggcacca ctgctctcct ggctgccgct ttccgccatg
    33001 gcagccctgc tgttgatggt ggcgtggaac atgagtgaag cgcacaaagt ggtcgacttg
    33061 ctgcgtcatg cgccgaaaga tgacatcatc gtcatgctgc tgtgtatgtc actgaccgtg
    33121 ctgtttgata tggttattgc catcagcgtg gggatcgtgc tggcatcgct gctgtttatg
    33181 cgtcgtatcg cacgtatgac tcgcctggca ccggtagtcg tagatgttcc agacgatgtc
    33241 ctggttctgc gcgttattgg cccgctgttt tttgctgctg ctgaaggctt attcacggac
    33301 ctggagtcac gtcttgaagg caaacggatt gtgattctga agtgggatgc cgttccggta
    33361 cttgatgctg gtggtcttga tgcgttccag cgttttgtga agcgtctgcc cgaaggatgt
    33421 gaactgcgcg tgtgcaacgt ggaattccag ccactgcgaa ctatggctcg cgcaggcatt
    33481 caaccgatcc cgggacgcct cgcgttcttc ccgaatcgtc gcgcggcgat ggcggattta
    33541 taagtacgag attgaccagt cagcgcaaac tgactggtca gcaaactgca ttatttgtta
    33601 gctatgacgg cgattatcgc tacggcggca acgtttgtca tagtgacgaa cccaccagta
    33661 acggtcgcac acctgttcat gcccgccaac acgggcaccc gccagccaga gaatggcacc
    33721 gacgaaaata cttagcactg caccatgtgc gaaatactgg ggcatattaa actgtggtaa
    33781 ctggttgagg attgaatacc ccacgccgac caccattacc accagaccca accccatgag
    33841 cacgttaccg agtatcgaag cgtttttgcg tttcatatgt cacctccgga actttctggg
    33901 ttgtaacagg gaatacccct cttccttatg tgtaaagtat agacaacacc ctgtgggtta
    33961 tgtgcgggcg taatcacaat tacaaccctt atttcaacaa aactttacaa ataaacgcct
    34021 gaacaccttg actttctaat agtccactca ggcaattatt acgttatctg catttgatac
    34081 gcagtttttt gtcaagcggg gccgcaacca gtaaactacg cgccagttat gtacacactc
    34141 aggacaaaaa aacgtgacga ttaaattgat tgtcggcctg gcgaaccccg gtgctgaata
    34201 cgccgcaacg cgacataatg ctggtgcctg gttcgttgac ttactggcag agcgtttgcg
    34261 cgctccgctg cgcgaagagg ctaaattctt tggttatact tcgcgagtca cccttggaag
    34321 cgaagatgtc cgcctgttag tcccgactac atttatgaat ctcagcggca aagccgttgc
    34381 ggcgatggcc agttttttcc gcattaatcc ggacgaaatt ctggtggccc acgacgaact
    34441 ggatctgcct cctggcgtcg ccaaatttaa gttgggcggt ggccatggtg gtcacaatgg
    34501 actgaaagac atcatcagta aattgggtaa taaccctaac tttcaccgtt tacgcatcgg
    34561 aatcggtcat ccgggcgata aaaataaagt tgtcggtttt gtgttaggca aaccgcctgt
    34621 tagtgaacag aagttaattg atgaagccat cgacgaagcg gcgcgttgta ctgaaatgtg
    34681 gtttacagat ggcttgacca aagcaacgaa ccgattgcac gcctttaaag cgcaataagt
    34741 cgttgtctgc ggcatttttg ccgagtgccg tgtataatag gcaaagttat ttccatttct
    34801 gcaatctgtt agcaataaca ggttgattat taagatatta aggtgattta aatcatggga
    34861 ttcaaatgcg gtatcgtcgg tttgcccaac gtcgggaaat ctaccctgtt caacgcgctg
    34921 accaaagccg gtattgaagc ggccaacttt ccattctgca ccattgagcc gaacacaggc
    34981 gtcgtaccaa tgcctgaccc tcgcctggat caactggctg aaatcgtaaa accgcaacgt
    35041 acgcttccca cgaccatgga atttgtcgat atcgccggtc tggtaaaagg cgcatcgaaa
    35101 ggcgaaggtc tgggtaacca gttcctgacc aacatccgtg aaaccgaagc gatcggtcac
    35161 gttgttcgct gctttgaaaa tgacaacatc attcacgttt ccggcaaagt taacccggct
    35221 gacgatattg aagttatcaa caccgaactg gcgctggcag acctcgacac ctgcgaacgt
    35281 gcgattcatc gcgtacagaa gaaagccaaa ggtggcgata aagacgcgaa agctgagctg
    35341 gcggtcctgg aaaaatgcct gccccagctg gaaaacgcag gtatgctgcg cgcgctggat
    35401 ttaagcgctg aagagaaagc ggctattcgt tacctgagct tcctgacgct gaaaccaaca
    35461 atgtacatag ccaacgtcaa cgaagatggt tttgaaaaca acccatatct tgaccaggtg
    35521 cgtgaaatcg cggcgaaaga aggttctgtt gtggttccgg tttgtgctgc tgttgaagca
    35581 gacattgccg aactggacga cgaagaacgt gacgagttta tgcaggagct tgggctggaa
    35641 gaaccgggcc tgaaccgtgt gatccgtgcc ggttataagc tgctgaacct gcaaacttac
    35701 ttcaccgctg gggtgaaaga agtgcgtgca tggaccattc cggttggagc aaccgcgccg
    35761 caggcagcgg gcaaaatcca tactgatttt gaaaaaggct ttatccgtgc acaaaccatc
    35821 tcgtttgaag atttcatcac ttacaaaggt gaacaaggcg cgaaagaagc aggcaaaatg
    35881 cgtgcagaag gtaaagatta catcgtgaaa gatggcgatg tgatgaactt ccttttcaac
    35941 gtctaattaa tttattgtct catgaggttt cgctaaatct catgaagagc gcaaaagtat
    36001 ataaaatcca cgcaattgcg tggatttttc atttcaccga gtcctaatca ttcttgtaac
    36061 atcctacact tttgtgctta ttgcctacag tgaaccacga gaataattaa agacataagt
    36121 gttcagatga ctgatatctc attagcagtg atataacgtc agtttttgtt ccactacaca
    36181 tcagccctct tcacagctga tcaatattga tctcgctcca atatgctggc gagaaaagga
    36241 cttacctgaa gggtaatatc ccgcagataa agtccccgaa taacgtgatt acgtgcgatc
    36301 ctggcaagat gaaaatgata accagtagca caataataac gataggccag aaaatccatt
    36361 gaacaaaaat gaatatcgat gttttttgct ttttcttatt agtaatgcaa aaatggcgca
    36421 tattgttttt gttatgcaga tacatcaagc gaaaatccca taactaatta ggattattac
    36481 aaaaaatctt caccaaattt tttaaccgct gtttgttgta atatttttgc catattcata
    36541 gtaacgaatt ggaataagta acgacaactt agggaataac tcgatataaa ctcacctttg
    36601 taaggggggg gttaaatata tattaattcc tgtgttcctg attttgcccg gtaatagaga
    36661 attattctca gagaagctca tcatgattgc accatacaga tatcatgatg ataagaggat
    36721 attattcatt gacttaaaca aaatgacaaa atccatagac ccatagcctg ggtactatat
    36781 aggagtggta tgggcatcaa acaacacaat gggaatacca aagccgatcg tctcgctgaa
    36841 ttaaaaatcc gttcgccctc aattcaactg ataaaatttg gcgctattgg tttgaatgca
    36901 attatctttt cccccctgct gatagctgct gatacaggaa gtcaatatgg caccaatatt
    36961 actattaatg atggtgacag aattacagga gataccgccg atccatcagg aaacctctat
    37021 ggtgtaatga ccccagcagg aaacacgcct ggcaatatca acctgggtaa tgatgtcacc
    37081 gtcaatgtca acgacgcctc tggatatgca aaaggaatca ttattcaggg caaaaacagc
    37141 tccctgacag ctaaccgact cacagtagat gttgttggtc aaacctctgc catcggcatt
    37201 aacttaattg gtgactatac ccatgctgac ttaggcacag gcagcaccat taagagtaac
    37261 gatgacggca tcattattgg gcatagctca acactaacag ccactcaatt caccattgaa
    37321 aactcgaacg gtataggcct aaccatcaat gactatggca ccagtgtcga tcttggaagc
    37381 ggaagtaaaa tcaagaccga tggaagtaca ggtgtttata tcggtggtct caacggcaat
    37441 aacgccaatg gtgctgcgcg ttttacggcg acagacctga caatcgatgt tcagggctac
    37501 agcgccatgg ggataaacgt acagaaaaac tctgttgtcg atctcggaac aaacagtacc
    37561 attaaaacca atggcgataa tgctcacggc ctctggagct ttggccaggt tagcgcgaat
    37621 gcactcactg ttgatgtaac tggagccgcg gccaatggcg tcgaagttcg tggtggtaca
    37681 accactatcg gtgcagatag ccatatttct tccgcgcagg gcggtggcct cgtcaccagt
    37741 agttcagacg cgacaatcaa tttttctggc acggcagcgc aacgaaacag catcttttcc
    37801 ggcggttctt atggtgcctc ggcccagacg gcaacggctg ttatcaacat gcaaaatacc
    37861 gatattacgg ttgatcgtaa tggcagtctg gcgctgggtt tgtgggcgct cagcggcggt
    37921 agaataaccg gagacagttt ggctatcacc ggcgcggcag gagccagagg gatttatgcc
    37981 atgaccaaca gccagatcga cctcacgagc gatctggtca ttgatatgag tacacccgac
    38041 cagatggcca tcgcaacgca acatgacgat ggttatgccg ccagccgcat caacgcctcg
    38101 ggtcgtatgc ttatcaacgg tagcgttctt tccaaaggtg ggctaatcaa tctggatatg
    38161 caccctgggt cggtttggac aggttcctcc ctcagcgata atgtcaatgg cgggaaactg
    38221 gacgttgcaa tgaataacag cgtctggaac gtaacaagta attctaatct cgacacgctg
    38281 gcgctgagcc attcaactgt cgattttgcc agccacgggt caactgccgg cacatttacc
    38341 acattaaacg tagagaacct gagcggtaac agtaccttta ttatgcgtgc tgatgttgtt
    38401 ggcgagggta atggcgttaa taatagaggg gatttattga atatcagcgg gagtagtgct
    38461 ggtaatcacg tattggctat ccgcaaccag ggcagcgagg ccacaacggg aaatgaagtt
    38521 ctgacagtgg taaaaaccac tgacggcgcg gcctcgttca gcgcgtcttc tcaggttgag
    38581 ttggggggat atctgtacga tgtgcgtaaa aatggcacta actgggagct ttacgcttcc
    38641 gggacagttc cggaaccgac tcctaatcct gaacccacac cagctcccgc tcagcctccc
    38701 atagtcaacc ccgatcctac gcctgaaccc gctcccacgc ctaaacccac cacgaccgca
    38761 gatgctggcg gcaattatct caatgtcggt tacttattga actatgttga aaaccgtacg
    38821 ctgatgcaac ggatgggtga cttgcgaaat cagagtaaag acggtaatat ctggttgcgc
    38881 agttatgggg gaagcctgga ctcctttgcc agtggcaaac tgagcggctt tgacatgggt
    38941 tacagcggta tccagtttgg tggggataaa cgtctctctg atgtaatgcc gttgtatgtc
    39001 ggtctgtata ttgactcaac acatgcatcg ccggactata gcggaggcga cggtaccgca
    39061 cgttcagact acatgggaat gtacgccagt tacatggcac aaaacggttt ttacagcgat
    39121 ctcgttataa aagcatcgcg ccagaaaaat agtttccacg tactggacag tcagaacaac
    39181 ggcgttaacg ccaacggcac tgcgaatgga atgagcatct ccctggaagc cgggcagagg
    39241 ttcaacctgt cccctactgg ttatgggttc tatatagagc cgcaaaccca gcttacatac
    39301 agccaccaga atgagatggc tatgaaggcg agtaatggcc tcaatataca tctgaatcac
    39361 tacgaatcgc tgctggggcg tgccagcatg atactggggt atgacatcac cgcaggcaac
    39421 agccagctga atgtctatgt gaagactggc gctatccgcg agttttcagg ggataccgaa
    39481 tatctgttga acgactcccg ggagaagtac agtttcaaag gtaatggctg gaataacggc
    39541 gtgggagtca gtgcacagta taacaaacag cacacattct atctcgaagc ggattacacg
    39601 cagggtaacc tctttgatca gaagcaagtc aacggaggat atcgcttcag cttttaagtg
    39661 cgggatagac aagcccttaa acgatgctga aaattgttag cgtgcgccgg aatgaaatac
    39721 agatcactct gatgtatcag aaatcgtttc tgtctttcat acccggcgct taaactgccc
    39781 tgcatcaatg ccatgttgct tcattttccg ccacagcgta gtgcggccga tcccaagtaa
    39841 agccgacatt tcctgaatgc gaccgcctgt gacctgggct gcgttaataa ttgcctcttt
    39901 ttcaacttcc gcaaatgaca gactggtgga aaggcgggtg gcgctgacat catctgtcgc
    39961 ctgctcggta aacagatgtt caggtaaatc actgacgcga atgcgcccgt tatcactact
    40021 cagagcaaga ttctcgatga cgctgtaaag ttcaaaatcg ttgcctggcc atgcacaaga
    40081 aactaggcga gcgagggcat cgtcatcaat tttcagccgc gtagagaagc gtttttcaag
    40141 actgcgtaat ttgttattca ccagcgccgg aatgctgcca cgccgcatac gcagaggcgg
    40201 gatggtaatt tcaaatgcat gcagcgcgta atacagctgg cgactaaaac gattttgttc
    40261 caccagcatt gcgaggtccg cggtcgttgt tgcaatcact ttgacatcaa ttggtattaa
    40321 acgccgcgca tccagtcgcg tgataacccc ctgcttgata acctgcagca aagcagactg
    40381 taactccacc gccagatatt caatcttttc aagaaacagc gtgccgccgt gtgccagttc
    40441 cagccgactc agacggccat tttcattgtc cgtgcgatcg ccaccaataa attcttccgc
    40501 cagcgcagca tcaccatata actcacaatt gacggcgata taaggacctg cagcacgctc
    40561 gctttcatta tgaattgcct gacttagcag tgccttgccc acgccctctt ctccacaaag
    40621 caggacagga aagctactgc gcgccgcctg gcgaccaaaa tgaatcaagc ggcgggtttg
    40681 cggatcgtcc tgtggcatat gagcgaaggt atggctgact tttcctaatt gactggtcat
    40741 taactgccgc atctgttcca caggatggag caacaaaata aagctggttc cctgcgtttc
    40801 tattatcggt ttaagggtta tcaccgcatc aataaactgg tgctggcttt caaaggttgc
    40861 ttctacgtgt ttgagcggat gtgccagttt tattgcttgt tgcaatacgg cgggtaacgt
    40921 taagagttca gtgattgccc gtccctgact tgccgtcgcg tcaaggcgca agacccgcgc
    40981 cgcctgggca ttaataaatt gcaaattacc ctgctcgtcc cagctaatca cgccatcatc
    41041 catactttct aacagggcat taagttgatt taaatgacgg ttagtttcag cgagcaaact
    41101 gtccgtcagc agtaaattac cgacctcgcg ggcgattgcc aacgtcaacg gcaaatcagc
    41161 tgcggtagtt tgctcaaccg gacacgccag cgctattgtt cccgtcaatc ggcccttgct
    41221 gtcaaacaac ggcgttgcac aaaaggccca gttccagagt gcctgtttga aatgttgatc
    41281 ggccatcgtt ttcacggcct gaccagagat agccgctaac gatagcgcac aagtaccaat
    41341 aattccctcg gcgcaatacg tgccgtcatt gaatcccagt gcacttagct gctgcaaggt
    41401 ttgcggatcg ccattacggc tgagaatgca ggcggtttca tcgaggataa acagcgcaca
    41461 ctctcgcggt gccatatatt cccaggcatc ttccagcgca gcctgcccga gcgtcagcat
    41521 cgctttctta cgccgataaa tagaagcaaa tgtcacgccc tgggcctgat gtggtacgtt
    41581 ccatgtctcc cgtttcatca gcttattgca tcgctcccag gaggttgcaa ttaagggaga
    41641 tatgccccga ccatcgttgt taaaagcgcc actcatatcc cgcatccctt atgacaaaag
    41701 gtgttccggc tcgcaatttt aagtattaat acacgtaaaa gtttcaatat ggaacattca
    41761 ttaaagcata tgtttcacta tgaaacataa tttgggctaa ttttcttttc ccttgcctga
    41821 tgcacaatgg attcatcggc agtcagtggt cgccgtgtcg ttgaacatca tccatgccct
    41881 accgtaattg ctggagcaaa ataatgaaaa aattgatcaa tgatgtgcaa gacgtactgg
    41941 acgaacaact ggcaggactg gcgaaagcgc atccatcgct gacactgcat caggatccgg
    42001 tgtatgtcac ccgagctgat gcccctgttg caggaaaagt cgccctgctg tcgggtggcg
    42061 gcagcggaca cgagccgatg cactgtggtt atatcggtca ggggatgctt tcgggggcct
    42121 gtccgggcga aattttcacc tcaccgacgc ccgataaaat ctttgaatgc tccatgcaaa
    42181 ttgatggcgg cgaaggtgta ctgttgatta tcaaaaatta caccggcgat attcttaact
    42241 ttgaaacagc gaccgagtta ctgcacgata gcggcgtaaa agtgaccact gtggtcattg
    42301 atgacgacgt tgcggtaaaa gacagtcttt ataccgccgg gcgacgcggc gttgccaaca
    42361 ccgtattaat tgaaaaactc gtaggcgcag cggcggagcg tggcgactca ctggacgcct
    42421 gtgcggaact ggggcgtaag ttgaataatc aaggccactc aataggtatc gctctcggtg
    42481 cctgtaccgt tcctgccgcg ggcaaacctt cttttaccct tgcggataat gagatggagt
    42541 ttggcgtagg cattcatggt gagccaggta ttgaccgccg ccctttctct tcccttgatc
    42601 aaaccgtcga tgaaatgttc gacaccctgc tggaacatgg ctcataccat cgcactttgc
    42661 gtttctggga ttatcaacaa ggcagctggc aggaagaacc acaaaccaaa caaccgctcc
    42721 agtctggcga tcgggtgatt gcactggtta acaatcttgg cgcaactccg ctttctgagc
    42781 tgtacggcgt ctataaccgc ctgaccacac gttgccagca agcgggattg actatcgaac
    42841 gtaatttaat tggcgcgtac tgcacctcac tggatatgac cggtttctca atcaccttac
    42901 tgaaagttga tgacgaaacg ctggctctct gggacgcccc ggtccacacc ccggccctta
    42961 actggggtaa ataaggagaa agcaatgtca ctgagcagaa ctcaaattgt taactggctc
    43021 actcgttgtg gcgatatttt caacaccgag agcgagtatc ttaccgggct ggatcgcgag
    43081 attggcgacg ctgaccacgg gctaaatatg aaccgaggct ttagcaaagt ggtggaaaaa
    43141 ctccctgcta tcgcagataa agatatcggt ttcattctca aaaataccgg tatgacgctg
    43201 ctttccagcg tcggtggtgc cagtggtccg ctgttcggta ccttctttat ccgcgccgca
    43261 caggcgaccc aggcacggca aagcctgaca ctggaagagc tttatcagat gttccgcgat
    43321 ggcgcggacg gcgtaatcag tcgcgggaaa gccgaacctg gcgataaaac catgtgtgat
    43381 gtgtgggtgc cggtggtgga atcgttacgt cagtccagcg agcaaaatct ctctgttccg
    43441 gtggcgctcg aagctgccag tagcatcgcc gaatccgctg cacaaagtac gattacgatg
    43501 caagcccgca aaggccgcgc cagttatctc ggtgaacgca gtattggtca ccaggatccc
    43561 ggcgcgacct cggtgatgtt tatgatgcaa atgttggcgt tagccgcaaa agagtaagga
    43621 attggtgatg gtaaacctgg tcatagtttc acatagcagc cgactgggag aaggtgtcgg
    43681 tgaattagcc cgtcagatgt taatgagtga tagttgtaaa atcgccattg ccgcgggaat
    43741 tgacgatcca caaaatccca ttggtaccga tgccgtcaaa gtgatggagg ccatcgaatc
    43801 tgttgctgat gccgaccatg tgctggtcat gatggatatg ggtagcgcat tattgagtgc
    43861 tgaaactgcg ctggaattgc tggctcccga gatcgccgca aaagtacgtt tgtgtgctgc
    43921 gccgttggtc gaaggtacac tggcagcaac ggtcagcgcg gcctcggggg cggatatcga
    43981 caaagttatc tttgacgcta tgcatgcgct ggaagccaaa cgtgaacaac tgggtttacc
    44041 gtcctccgac actgaaatct ctgacacatg tcctccgtac gatgaagaag cccgttctct
    44101 ggcggtggtc ataaaaaacc gtaacggcct gcatgtacgt ccggcctccc ggctggttta
    44161 taccttatcg acatttaatg ccgatatgtt gctggaaaaa aacggcaaat gcgtcacacc
    44221 agagagtatt aaccagattg cgttactaca agttcgctat aacgatacgc tgcgcctgat
    44281 tgcgaaaggg ccagaagctg aagaggcact gatcgctttc cgtcacctgg ctgaagataa
    44341 ctttggtgaa acggaggaag tcgctccacc tactctgcgt cccgttctgc ctgtttcggg
    44401 taaagccttt tattatcaac cagttttatg tacggtacag gcaaaatcaa ccctgaccgc
    44461 ggaagaagaa caagatcgat tacgccaggc tattgacttc acgttattag atctgatgac
    44521 gttaacagcg aaagcagaag ccagcgggct tgacgatatt gccgcaatct tttctggtca
    44581 ccatacactg ttaggtgatc cggaactgct ggcggcggca agcgaactcc ttcagcatga
    44641 acattgcacg gcagaatatg cctggcagca agttcttaaa gaacttagcc agcaatacca
    44701 gcaactggat gatgaatatc tacaagctcg ctatattgat gtggacgatc ttctgcatcg
    44761 caccctggtc cacctgaccc aaacgaaaga agaactcccg cagtttaact cgccaactat
    44821 tctactggcg gagaacattt atccttccac agtactgcaa ctggatccgg cggttgtaaa
    44881 aggtatctgc cttagcgccg gaagtccggt atcccacagc gccctaatcg cccgtgaact
    44941 ggggattggc tggatttgcc agcagggtga gaaactgtat gcgatacaac cagaagaaac
    45001 gctaacgctg gacgttaaaa cgcaacgttt caaccgtcag ggttaagcca ccgttcggat
    45061 ggcatcgttc tgatgtcatc cggcaattta cccagcactg atgtttttta actacctgtt
    45121 atatttttta ccaatttatg atgatttttc ttcgctaatc gcgaaccaaa tgtcgctggc
    45181 tggctaataa acagacgtta tttgatcact ctatttcgcc gttatttatc cctttaattc
    45241 ccttttctaa aatgcctgac cgttcgcaga atgagatttc gatcatgcag ctagtgcgat
    45301 cctgaactaa ggttttctga tacttgaata ccgttttatt cggtttcgcc aaaggagaat
    45361 gattgatgaa atcccccgca ccttctcgcc cgcaaaaaat ggcgttaatt ccagcctgta
    45421 tctttttgtg tttcgctgcg ctatcggtgc aggcagaaga aacaccggta acaccacagc
    45481 cgcctgatat tttattaggg ccgctgttta atgatgtgca aaacgtcaaa ctttttccgg
    45541 accaaaaaac ctttgccgat gccgtgccta acagcgatcc gctgatgatc cttgctgatt
    45601 atcggatgca gcaaaaccag agcggatttg atctgcgcca tttcgttaac gtcaatttca
    45661 ccctgccgaa agaaggcgag aaatatgttc cgccagaggg gcagtcactg cgcgaacata
    45721 ttgacggact ttggccggta ttaacgcgtt ctaccgaaaa caccgaaaaa tgggattctc
    45781 tgttaccgct gccggaacct tatgtcgtgc cgggcggacg ttttcgcgag gtatattact
    45841 gggacaatta cttcaccatg ttaggacttg ccgaaagtgg tcactgggat aaaatcgcgg
    45901 atatggtggc caattttgct catgaaataa acacttacgg tcatattccc aacggcaacc
    45961 gcagttacta tttaagccgc tcgcaaccgc ccttctttgc cctgatggta gagttactgg
    46021 cgcagcatga aggcgatgcc gcgttgaagc aatacctgcc gcaaatgcaa aaagaatatg
    46081 cttactggat ggacggtgtt gaaaacctgc aagccggaca acaggaaaaa cgcgttgtaa
    46141 aacttcagga tagtaccctt ctcaaccgct actgggacga tcgcgatacg ccacgaccag
    46201 agtcatgggt ggaagatatt gccaccgcca aaagcaatcc gaatcgacct gccactgaaa
    46261 tttaccgcga cctgcgctct gccgctgcgt ctggctggga tttcagctcg cgctggatgg
    46321 acaacccgca gcagttaaat accttacgca ccaccagcat cgtaccggtc gatctgaaca
    46381 gcctgatgtt taaaatggaa aaaatcctcg cccgcgccag caaagctgca ggagataacg
    46441 cgatggcaaa ccagtacgaa acgctggcga atgcccgtca aaaagggatc gaaaaatacc
    46501 tgtggaacga tcaacaaggc tggtatgccg attacgacct gaaaagtcat aaagtgcgca
    46561 atcagttaac cgcggccgcc ctgttcccgc tgtacgtcaa tgcggcagcg aaagatcgcg
    46621 ccaacaaaat ggcgacggcg acgaaaacac atctgctgca acccggcggc ctgaacacca
    46681 cgtcggtgaa aagtgggcaa caatgggatg cgccaaatgg ctgggcaccg ttacagtggg
    46741 tcgcgacaga aggattacaa aactacgggc aaaaagaggt ggcgatggac attagctggc
    46801 acttcctgac caatgttcag cacacctatg accgggagaa aaagctggtg gaaaaatatg
    46861 atgtcagcac caccggaacg gggggcggcg gtggcgaata tccattacag gatggctttg
    46921 gctggaccaa tggcgtgacg ctgaaaatgc tggatttgat ctgcccgaaa gagcaaccgt
    46981 gtgacaatgt tccggcgacg catccgaccg ttaagtcagc aacgacgcaa ccctcaacca
    47041 aagaggcaca acccacacct taaccagcgc ttactccgac aacgtcattc ggcttctccg
    47101 gatggcgttg tcggcagata acaccatatc tatggttttt tctacacaga cggtttttca
    47161 agtcctggcg ggaagtattg cgcaaatgtt tgccatacgt ggttgccgaa gatagcgtca
    47221 tttctacggg taagtgtttc actttcatca cccggcgatt tacgccgacg ttggctatat
    47281 aaagcatcaa ttgctaccga atattgttac ggtgttcact catcaacgtt tttttgcttt
    47341 ctggagttaa attaataatc acataatcca gacactccag agaatttagc aaccaaaaaa
    47401 agaattcaga ccattcctct tcgcgtaact gaagcattgc cgaacgggta ttttggctta
    47461 atctgccaga aggattgatc aattctaaat gttcaataag cgcagccaaa accgcctcac
    47521 tatccgcccc atttttaacg cctgactggc tactgtctaa aatatttatt ttctgaatca
    47581 caccaaaaac aacgaaacaa taattaaaaa aggattctgt tgttgttacc gggaaccgac
    47641 ttatttcgct cagaaagtcg ttcaaccagc tccgcagtga taagattcgc tgatgataca
    47701 gtgtatcctc actgcgtgct ggaggtgcca gtataatatg ttgatcaata cagtatttat
    47761 tgtgttcagt cgtttcctga tgcgtttggt tctctgcgga ttgtcgctgg cgaaataaat
    47821 ggcttgcggt aaatgccgtt acagagcagc gcaacgcgtc aattccttcg gagtgcccga
    47881 cttttggcga gagttccggt tttatgcgtc caggtagcgg aaatttcacc cgaatgagaa
    47941 atgcattaat aaaat
//
LOCUS       DAJTSI010000013        45785 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000013 DAJTSI010000000
VERSION     DAJTSI010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45785
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.013"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            95..2248
                     /locus_tag="OO327_000664"
     CDS             95..2248
                     /locus_tag="OO327_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR6017321.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2301..4031
                     /locus_tag="OO327_000665"
     CDS             2301..4031
                     /locus_tag="OO327_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6017322.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYNSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(4091..4582)
                     /gene="yajQ"
                     /locus_tag="OO327_000666"
     CDS             complement(4091..4582)
                     /gene="yajQ"
                     /locus_tag="OO327_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR6017323.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4750..5661
                     /gene="panE"
                     /locus_tag="OO327_000667"
     CDS             4750..5661
                     /gene="panE"
                     /locus_tag="OO327_000667"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR6017324.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5624..6214
                     /gene="yajL"
                     /locus_tag="OO327_000668"
     CDS             5624..6214
                     /gene="yajL"
                     /locus_tag="OO327_000668"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR6017325.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6268..7716)
                     /gene="thiI"
                     /locus_tag="OO327_000669"
     CDS             complement(6268..7716)
                     /gene="thiI"
                     /locus_tag="OO327_000669"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR6017326.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7922..8164
                     /gene="xseB"
                     /locus_tag="OO327_000670"
     CDS             7922..8164
                     /gene="xseB"
                     /locus_tag="OO327_000670"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR6017327.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8164..9063
                     /gene="ispA"
                     /locus_tag="OO327_000671"
     CDS             8164..9063
                     /gene="ispA"
                     /locus_tag="OO327_000671"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR6017328.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9088..10950
                     /gene="dxs"
                     /locus_tag="OO327_000672"
     CDS             9088..10950
                     /gene="dxs"
                     /locus_tag="OO327_000672"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR6017329.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            11005..11922
                     /gene="yajO"
                     /locus_tag="OO327_000673"
     CDS             11005..11922
                     /gene="yajO"
                     /locus_tag="OO327_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR6017330.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(12033..12551)
                     /gene="pgpA"
                     /locus_tag="OO327_000674"
     CDS             complement(12033..12551)
                     /gene="pgpA"
                     /locus_tag="OO327_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR6017331.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12529..13506)
                     /gene="thiL"
                     /locus_tag="OO327_000675"
     CDS             complement(12529..13506)
                     /gene="thiL"
                     /locus_tag="OO327_000675"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR6017332.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13584..14003)
                     /gene="nusB"
                     /locus_tag="OO327_000676"
     CDS             complement(13584..14003)
                     /gene="nusB"
                     /locus_tag="OO327_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR6017333.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14023..14493)
                     /gene="ribE"
                     /locus_tag="OO327_000677"
     CDS             complement(14023..14493)
                     /gene="ribE"
                     /locus_tag="OO327_000677"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR6017334.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14582..15685)
                     /gene="ribD"
                     /locus_tag="OO327_000678"
     CDS             complement(14582..15685)
                     /gene="ribD"
                     /locus_tag="OO327_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR6017335.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15689..16138)
                     /gene="nrdR"
                     /locus_tag="OO327_000679"
     CDS             complement(15689..16138)
                     /gene="nrdR"
                     /locus_tag="OO327_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR6017336.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16289..16828
                     /locus_tag="OO327_000680"
     CDS             16289..16828
                     /locus_tag="OO327_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR6017337.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            17126..18010
                     /gene="tsx"
                     /locus_tag="OO327_000681"
     CDS             17126..18010
                     /gene="tsx"
                     /locus_tag="OO327_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR6017338.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18187..18534)
                     /locus_tag="OO327_000682"
     CDS             complement(18187..18534)
                     /locus_tag="OO327_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR6017339.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18663..19634)
                     /gene="secF"
                     /locus_tag="OO327_000683"
     CDS             complement(18663..19634)
                     /gene="secF"
                     /locus_tag="OO327_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR6017340.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19645..21492)
                     /gene="secD"
                     /locus_tag="OO327_000684"
     CDS             complement(19645..21492)
                     /gene="secD"
                     /locus_tag="OO327_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR6017341.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21520..21852)
                     /gene="yajC"
                     /locus_tag="OO327_000685"
     CDS             complement(21520..21852)
                     /gene="yajC"
                     /locus_tag="OO327_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR6017342.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21875..23002)
                     /gene="tgt"
                     /locus_tag="OO327_000686"
     CDS             complement(21875..23002)
                     /gene="tgt"
                     /locus_tag="OO327_000686"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR6017343.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(23057..24127)
                     /gene="queA"
                     /locus_tag="OO327_000687"
     CDS             complement(23057..24127)
                     /gene="queA"
                     /locus_tag="OO327_000687"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR6017344.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24220..24793
                     /gene="acpH"
                     /locus_tag="OO327_000688"
                     /pseudo
     CDS             24220..24793
                     /gene="acpH"
                     /locus_tag="OO327_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24798..26615)
                     /gene="malZ"
                     /locus_tag="OO327_000689"
     CDS             complement(24798..26615)
                     /gene="malZ"
                     /locus_tag="OO327_000689"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCR6017345.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26771..28144)
                     /gene="proY"
                     /locus_tag="OO327_000690"
     CDS             complement(26771..28144)
                     /gene="proY"
                     /locus_tag="OO327_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR6017346.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28220..29539)
                     /gene="brnQ"
                     /locus_tag="OO327_000691"
     CDS             complement(28220..29539)
                     /gene="brnQ"
                     /locus_tag="OO327_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR6017347.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29946..31241)
                     /gene="phoR"
                     /locus_tag="OO327_000692"
     CDS             complement(29946..31241)
                     /gene="phoR"
                     /locus_tag="OO327_000692"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR6017348.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31299..31988)
                     /gene="phoB"
                     /locus_tag="OO327_000693"
     CDS             complement(31299..31988)
                     /gene="phoB"
                     /locus_tag="OO327_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR6017349.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            32178..33380
                     /gene="sbcD"
                     /locus_tag="OO327_000694"
     CDS             32178..33380
                     /gene="sbcD"
                     /locus_tag="OO327_000694"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR6017350.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPTPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33377..36520
                     /gene="sbcC"
                     /locus_tag="OO327_000695"
     CDS             33377..36520
                     /gene="sbcC"
                     /locus_tag="OO327_000695"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR6017351.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHVRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36646..37827
                     /gene="araJ"
                     /locus_tag="OO327_000696"
     CDS             36646..37827
                     /gene="araJ"
                     /locus_tag="OO327_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR6017352.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37970..38878)
                     /gene="mak"
                     /locus_tag="OO327_000697"
     CDS             complement(37970..38878)
                     /gene="mak"
                     /locus_tag="OO327_000697"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR6017353.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            39003..39914
                     /gene="rdgC"
                     /locus_tag="OO327_000698"
     CDS             39003..39914
                     /gene="rdgC"
                     /locus_tag="OO327_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR6017354.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(39992..40447)
                     /locus_tag="OO327_000699"
     CDS             complement(39992..40447)
                     /locus_tag="OO327_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6017355.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40444..40779)
                     /locus_tag="OO327_000700"
                     /pseudo
     CDS             complement(40444..40779)
                     /locus_tag="OO327_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40934..41218)
                     /gene="ppnP"
                     /locus_tag="OO327_000701"
     CDS             complement(40934..41218)
                     /gene="ppnP"
                     /locus_tag="OO327_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR6017356.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41290..41967)
                     /locus_tag="OO327_000702"
     CDS             complement(41290..41967)
                     /locus_tag="OO327_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR6017357.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42225..42416)
                     /gene="yaiA"
                     /locus_tag="OO327_000703"
     CDS             complement(42225..42416)
                     /gene="yaiA"
                     /locus_tag="OO327_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR6017358.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(42466..42990)
                     /gene="aroL"
                     /locus_tag="OO327_000704"
     CDS             complement(42466..42990)
                     /gene="aroL"
                     /locus_tag="OO327_000704"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR6017359.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43173..43631)
                     /locus_tag="OO327_000705"
     CDS             complement(43173..43631)
                     /locus_tag="OO327_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR6017360.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43751..44560
                     /gene="proC"
                     /locus_tag="OO327_000706"
     CDS             43751..44560
                     /gene="proC"
                     /locus_tag="OO327_000706"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR6017361.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44577..45692)
                     /gene="adrA"
                     /locus_tag="OO327_000707"
                     /gene_synonym="dgcC"
     CDS             complement(44577..45692)
                     /gene="adrA"
                     /locus_tag="OO327_000707"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR6017362.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        11899 a        11944 c        11203 g        10739 t
ORIGIN      
        1 tcgcttaatt ccatataact ggacgattac attagaagta aactatactt tttatgaaat
       61 atcagggata aattattcac atggaaaata catcatgtac aaatcattat ttactattat
      121 cattctactg tatagcgcca gttcatatgc aaaaagcgaa tcccctcccg caagcactat
      181 aactttcatt gcacaaatcg atcatgttga gctaaataaa acagcattat ctcatgatat
      241 gaaactcgtt cttgccgatc gtttttattt caaaaccagt aatccttgcg aaacaaattc
      301 aatttctgct cgaccagaat caatggatat tactgacaaa gcctatgccg acaaattaaa
      361 aaaattagaa actccttttc ttgatgatcg ctatttcttt atgaccatca atcagtgtga
      421 cggtaatggc actcccatgc tgacagacat aaaagtatgt acaacagaat tatgtggtgc
      481 tgaatataca aagaaaatgt ctactgtatg gctcgatgaa actttaaaac caactgctaa
      541 acgtctggca atttatttta ttcaatctcc tttaccctat gacgaggaga aaaaacaatg
      601 gaaagtcacc ggatggtata tctcatcaac tgataatcag tcttcaacta aacagattgc
      661 atttgaagga tatacagaca cagataacct ctccgattct aaatttattt cttcatttaa
      721 atcctggttt gaaagtggaa agataaaaaa cattgctaac tataatccaa aaggagaaag
      781 agatggaaaa tacgaaagtt attacaatag caatggtaac gtctatgaat ctctaagttt
      841 ttctaatggt cttatcaacg gtgagtttat tacatattat gaaaatgggg ccattgaaag
      901 taaacgtcat tttaataacg acaaaattaa agatggtgaa tgccctcatt tttatgaaga
      961 tgggaaaatc aaacagcaac atagctatct gaatagtaaa ctcgatggtc ctgcatatga
     1021 atattatcct gatggcaaac tcatgcagga aaatttctat cagcaaagtg tgttgattgg
     1081 aaacgatacc agttactacc aatccggaaa aataagctct atacacaata gaaattcccg
     1141 tggtcagtat gatggagcta acgaacgtta tagtgaagag ggtaagttac tgtctaaatc
     1201 cgtctataaa gacggaaaac aaatttctgt tcaaacctgg tatgaaaatg ggcagaaaga
     1261 agaggagaaa cattttgatg agcaaggcca attaaatggt ctggttaagc aatggtataa
     1321 aaatggtaat cttgcaaaat cacaaaacta taaacatgac atactcgatg gtaacagtga
     1381 agagtggtac gaaaatggca tccctgaaag tttgtaccct tataagaatg gaaaaaccga
     1441 cggcattgcc aaatcatgga ataagtatgg taaattaacc tattcaatcg aatacaaaaa
     1501 tggcgtagag aatggggcat atcgtaactg gagcaaaaac acaggtaaac taaccaaaga
     1561 aaccctgtac gtcaatggta tcagacaggg agtggaaaaa gagtttaacg atcgcacagg
     1621 taaactatta acctccacgc aatatgttaa taataaaaga catggtacgg aggaaactta
     1681 tgatcaaaac ggaataaaat atattacatg ctatcaaaat gatcagaaat tatcgtcttt
     1741 agacaatcca acacaaataa aagataatgc aacaacaggc gattccagtg ctcaatttgc
     1801 attagggaaa tacgaattca tctgtgcaaa tatagatgaa gggattaaat ggttaaccaa
     1861 atctgcagag caaaaaaaca ctgatgcgat atacttttta gctacagcct acaaaggcaa
     1921 tggtatacct gcaaacaatg aaaaatacct agcatatctg caacaagctg caacactggg
     1981 cagtagtaat gcccaggcgg aaattggcta tttatatctc atcggaaaag aactaccaca
     2041 gaatcttcct gatgctggtg tctggttcaa aaaagcagct gcacaaggta attttgccgc
     2101 acatttctat ctgggaagga tgtatcagaa tggtgacggc gttgagagaa atatggaaaa
     2161 agccaggttt catctatcta atgcggcaga aggtggaata gaacctgcgc ttaaagcatt
     2221 aaatgaactt gaacatcaaa caaaataaag cgctcaggga ccacgtaagg ttcatcaaac
     2281 agaaaaggat atagtttgcg atgaaagcga aatccacttt gttattaatg attttatctg
     2341 ttttttattc tcatcaggtt ttatccagcg aacccactca gccggatgtt tattctgtcg
     2401 tcgagaaaaa actaaactct gctttacctc tggaagaaaa taccaccttc agatcgcagg
     2461 ctgaatggtt taatttgcaa tatgaattat tagtatctgg ctaccctgaa cgtgcttaca
     2521 atttactgag caaacttgag acagaatcga aaaacgctaa atattatctg gatttaactg
     2581 cctccagagt cgctgaagaa gaagcaccag agacagcctt aactttttta tcaaaaattg
     2641 ggttaaataa accggctact cttacatcct ttgagagcta tatcaatagc tggataaaaa
     2701 gcaaacaacc cgaaaaggct atgcagcttc tcagcctcga tgagaatgcc ctgaattatt
     2761 atttaccgac tgtgttagtc gcatatcaga tcgttcctga taaggcaata agtatttata
     2821 attctgtata tggagataat gttgttatac cctatcagca agtcaccatg ttacttacag
     2881 ttgcggaggg ttatcaaaca aaaggcgata taaaaaacgc cattcattat gccgataagg
     2941 cgctgaatat gtttgataaa gctattgctg tagatagcag tcgtgaatcc cattatagtg
     3001 atgagtattt gaaactcatg aatatctacg ccgccaatgg caacaaagaa aaagcggtgg
     3061 ttttatctca acgtctacac aaagccatta ccgaatctca atcgaattac ttaagtactc
     3121 tggaaggaat tttattgttt tatagaaata atgatatgca gcaagactat caaaacagcc
     3181 tttcaaatta catagtattt atcgataaaa tttttgcctt ttccccatca acaagaagtg
     3241 aattaagcct cattaattta ttaaattcat taaatgaggt ggaattgatg agaaaacgtc
     3301 tggcgtttct gatgacagca ccagaatacg cttgttataa tagtcagtat tgttatgaag
     3361 acaaagtcag cgcattaaaa atcctgtatc gtcatcatga caatgagcta atggacaaat
     3421 atttcacgac tcttcttaaa gagatcagta atcaaccatt agaagactgg gatataataa
     3481 taagtaatac tggtgaaaaa tttagtgaag tgggtctaac caaatatgct caacatctcg
     3541 catctttcgc tgaagaattt tatataaaag agcaaaaatc acattctgaa agtgaaatga
     3601 gaggactatt ttcatcactt gccgaattat atagttttgg taatgatact gccagcgcaa
     3661 accgagtgtt ccatcagcat gtccctgcct tcaatacaga tcatatgatt caatatttta
     3721 taaatgccaa agaatggaac aatgccaggg aattattgat aaaagaggaa atgttgggta
     3781 tgcataatct gatattgttg gagaatattt gtatgcaaaa aaatgcagaa tgcatggcac
     3841 acattacctt tacgcttaat aaattaacaa cacaaccagc aataacaaaa atagatagcg
     3901 tgggtaatga gcaactttat cagattggca aaatctacca taagttggaa attaaacctg
     3961 agccagagca acaggtttta attcagtcgc tttatgaccg ggcatcagga agcgcctctg
     4021 caactcaata aatgcatggt caaaagggcc accacggtgg ccctgacaat cagtagctat
     4081 atccagaaag ttaatcgcgg aagtttttga actggaacgg ctgaccgaga tcgccaccgc
     4141 gcaccatcgc catgacagcc tgcaaatcat cacgagattt gccagtcacg cggatctcat
     4201 cgccctgaat ttgcgcctgc actttcagtt tgctgtcttt gatcatcttg acgatttttt
     4261 tctgagtggc gctctcaatg ccctgtttca gtttcgcttc cacaaaccag gttttaccgc
     4321 tatgaacgat attttccggt acatccagtg aactgccttc aatgccgcgc ttcagcagct
     4381 tggcacgcag aatatccagc aactgattga cctggaagtc ggactcgctc aacactttga
     4441 tggttttgct ggcgtcgttc agctcaaatg aggcttcaac gttacggaag tcaaaacggg
     4501 actccacttc gcgactcgcg ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag
     4561 agacaatatc gaaagatggc atcttttcct ctcccttcat ttttgatgcg aagcataata
     4621 cctgcaaagt taaataacca acagtcctgc accaggacgt atcccttaat tgtgcatagt
     4681 cgcaacagca tgcacaatta agggatagcc ttattgaaca cccggagtgg ttgcgggtga
     4741 ggaggaacaa tgaaaattac cgtattggga tgcggtgcct tagggcaatt atggcttaca
     4801 gcactttgta aacagggtca tgaagttcag ggctggctgc gtgtaccgca accttattgt
     4861 agtgtgaatc tggttgagac agatggttcg atatttaacg aatcgctgac cgccaacgat
     4921 cccgattttc tcgccaccag cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc
     4981 gatgccgtca aaagcctcgc atccacactg cctgtaacta cgccaatact gttaattcac
     5041 aacggcatgg gcaccatcga agaattgcaa aacattcagc agccattact gatgggcacc
     5101 accacccatg ccgcccgccg cgacggcaat gtcattattc atgtggcaaa cggaatcacg
     5161 catatcggtc cggcacggca acaggatggc gattacagtt atctggcgga tattttgcaa
     5221 accgtgttgc ctgacgttgc ctggcataac aatattcgcg ccgagctgtg gcgcaagctg
     5281 gccgtcaact gcgtgattaa tccactgact gccatctgga attgcccgaa cggtgaatta
     5341 cgtcatcatc cgcaagaaat tatgcaaata tgcgaagaag tcgcggcagt gatcgaacgc
     5401 gaagggcatc atacttcagc ggaagatttg cgtgattacg tgatgcaggt gattgatgcc
     5461 acagcagaaa atatctcgtc gatgttgcag gatatccgcg cgctgcgcca caccgaaatc
     5521 gactatatca atggttttct cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa
     5581 aacacccgcc tgtttgaaat ggtaaaaaga aaggagagtg aatatgagcg catcggcact
     5641 ggtttgcctc gcccctggta gtgaagagac tgaagccgtc accacgatca acctcttggt
     5701 tcgcggcggt atcaaagtca ccactgccag cgtcgacagc gatggcaacc tggcaattac
     5761 ctgctcgcgc ggcgtgaagc tgctggcgga tgcgccgctg gtcgaagtgg ctgatggcga
     5821 atatgacgtg atcgtgctgc ctggtggcat taaaggcacg gagtgttttc gcgatagcac
     5881 tctgctggtt gaaaccgtta aacagttcca ccgttccggg cgtatcgtcg cggctatttg
     5941 cgccgcgcca gccaccgtgc tggtgccgca cgatatcttt ccgattggca atatgaccgg
     6001 cttcccgacg ctgaaagaca aaattcccgc tgaacaatgg caggacaagc gcgtcgtctg
     6061 ggatgcacgg gtaaaattgc tgaccagcca ggggccgggt acagctatcg actttggtct
     6121 gaaaattatc gacctgctgg ttgggcgcga gaaagcccat gaagtggcat cacaactggt
     6181 gatggcggca gggatttata attattacga gtagtgtcgg atgcggcaaa cgtcgcatct
     6241 gaccagatgc gacgtaaaaa ctacaaatta cgggcgatat accttcacat tgttaaagcc
     6301 ttgctcgcgc agatagagcg cctgcagacg gctcatcacc ccgcgctcac accacagcag
     6361 ccaggttttg ttctggtcga gatcgccaaa tttggtgctc agtttataga acggcagaga
     6421 aaccacatca atcccttcga ctttcagtgg cttatcttcc tgttcatcga cagaacggat
     6481 atcgagaatc acgtcgttcg ggccgaagcc gttgacggtt tccacttcca ccacttcctg
     6541 ctcggtctgc tgggcgattt cgcggatatc aacgttattc gcttcctcaa ccactttatc
     6601 gagaatactg aagtcgaact tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac
     6661 cgtcgggctt ttggagatca cgccacaata ttccggcatc gtgcgagcaa agtcttcggt
     6721 gccaatctgg cgagccaggt tgatgatgtg ctctttgtcg taagaaatca gcggacgcag
     6781 gatcagcgtg tcagagacgt tatcaatcag gcgcaggttg gtcagcgtct gactggacac
     6841 ctggccgagc gcttcgccgg tgaccagcgc ctgtacgccg taacgttcag ccactttaga
     6901 tgcggcacgc accatcatac gtttgaggat aacgcccatc tgaccgtcgt cgattttctc
     6961 gagaatttcc ccgacaaccg gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct
     7021 gccaaagcgg ttccacagat aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc
     7081 gccgaggtta aagaagcagt aatgcacgcg gcagccgcga cgcatcaaca tataactgga
     7141 aacaccggag tcgaaaccac cggaaatgag cgacagcaca tcttcctggg tgccaatcgg
     7201 gaaaccacca ataccttcgt agcgaccttt aatcagcagg aggcgatcgt cttccacttc
     7261 cagatgaaca gttacatccg ggttagtcag cttcacgcgc gcggattcaa tatgctgatt
     7321 taaaccgccg ccgacgtaac gttccacatc aatcgagcta aaatcatgtt tgccacggcg
     7381 cttcacgcgt acgcagaagg ttttgccttc cagctgatcg cgatactgaa ccaacgcttt
     7441 ctcaaaaata tcgtgcatgt cggtaaacgg cacgtcttcg acttcgagaa tatggtggat
     7501 acccggaata cgggtcaggg cgtcgcgaat agtcagacgc tggttttcat ctttcgcgcg
     7561 aacttcgatg ttatcccagt ggcggacgac agcgagcgtc tcatcatagt gctttaaaac
     7621 gttacgaatg ttcccggtaa ggatttttat aaagcgcaag cgcacagatt ggcttttgat
     7681 ggtgatttcc gggaacaatt taatgataaa cttcatggcg gcaatggttc gttggcaagc
     7741 cttaagcgac ttgtataggg aaaaatacag cagcccacac ctgcggctgc atccaggcgc
     7801 ggaagtatac cactaacatc gctttgctgc gcacatcacc ttaccattgc gcgttatttg
     7861 ctatttgccc tgagtccgtt accatgacgg ggcgaaaaat attgagagtc agacattcat
     7921 tatgccgaag aaaaatgagg cgcccgccag ctttgaaaag gcgctgagcg agctggaaca
     7981 gattgtaacc cgtctggaaa gtggcgacct gccgctggaa gaggcgctga acgagttcga
     8041 acgcggcgtg cagctggcac gtcaggggca ggccaaatta caacaagccg aacagcgcgt
     8101 acaaattctg ctgtctgaca atgaagacgc ctctctaacc ccttttacac cggacaatga
     8161 gtaatggact ttccgcagca actcgaagcc tgcgttaagc aggccaacca ggcgctgagc
     8221 cgttttatcg ccccactgcc ctttcagaac actcccgtgg tcgaaaccat gcagtatggc
     8281 gcattattag gtggtaagcg cctgcgacct ttcctggttt atgccaccgg tcatatgttt
     8341 ggcgttagca caaacacgct ggacgcaccc gctgctgccg tagagtgtat ccacgcttac
     8401 tcattaattc atgatgattt accggcgatg gatgatgacg atctgcgtcg cggtttgccg
     8461 acctgccatg tgaagtttgg cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg
     8521 ctggcgttct cgattctaag cgatgccaat atgccggaag tgtcggatcg cgacagaatt
     8581 tcgatgattt ctgaactggc gagcgccagc ggtattgccg gaatgtgcgg tggtcaggca
     8641 ttagatttag acgcggaagg caaacacgta cctctggacg cgcttgagcg tattcatcgt
     8701 cataaaaccg gcgcattgat tcgcgccgcc gttcgccttg gtgcattaag cgccggagat
     8761 aaagggcgtc gtgctctgcc agtactcgac aagtacgcag agagcatcgg ccttgccttc
     8821 caggttcagg atgacatcct ggatgtggta ggagatactg caacgttggg aaaacgccag
     8881 ggtgccgacc agcaacttgg taaaagtacc taccctgcac ttctgggtct tgagcaagcc
     8941 cggaagaaag cccgggatct gatcgacgat gcccgtcagg cgctgaaaca actggctgaa
     9001 cagtcactcg atacctcggc actggaagcg ctagcggact acatcatcca gcgtaataaa
     9061 taaacaataa gtattaatag gcccctgatg agttttgata ttgccaaata cccgaccctg
     9121 gcactggtcg actccaccca ggagttacga ctgttgccga aagagagttt accgaaactc
     9181 tgcgacgaac tgcgccgcta tttactcgac agcgtgagcc gttccagcgg gcacttcgcc
     9241 tccgggctgg gcacggtcga actgaccgtg gcgctgcact atgtctacaa caccccattt
     9301 gaccaattga tttgggatgt gggacatcag gcttatccgc ataaaatttt gaccggacgc
     9361 cgcgacaaaa tcggcaccat ccgtcagaaa ggcggtctgc acccgttccc gtggcgcggc
     9421 gaaagcgaat atgacgtatt aagcgtcggg cattcatcaa cctccatcag tgccggaatt
     9481 ggtattgcgg ttgctgccga gaaagaaggc aaaaatcgcc gcaccgtctg tgtgattggc
     9541 gacggcgcga ttaccgctgg catggcgttt gaagcgatga atcacgcggg cgatattcgt
     9601 cctgatatgc tggtggtcct caacgacaat gaaatgtcga tttccgaaaa tgttggcgcg
     9661 cttaacaacc atctggctca gctgctttcc ggtaagcttt actcttcact gcgcgaaggc
     9721 gggaaaaaag ttttctctgg cgtgccgccc attaaagagc tgctcaaacg taccgaagaa
     9781 catattaaag gcatggtggt gcctggcacg ttgtttgaag agctgggatt taactacatc
     9841 ggcccggtgg acggtcacga tgtgctgggg cttatcacca cgctaaagaa catgcgcgac
     9901 ctgaaaggcc cgcagttcct gcatatcatg accaaaaaag gtcgtggtta tgaaccggca
     9961 gaaaaagacc cgatcacttt ccacgccgtg cctaaatttg atccctccag cggttgtttg
    10021 ccgaaaagta gcggcggttt gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa
    10081 acggcagcga aagacaacaa gctgatggcg attactccgg cgatgcgtga aggttccggc
    10141 atggtcgagt tttcacgtaa attcccggat cgttacttcg acgtggctat tgccgagcaa
    10201 cacgcagtga cctttgccgc cggtctggcg attggtggat acaaacccat tgtcgcgatc
    10261 tactccacct tcctgcaacg cgcctatgat caggtgctgc atgacgtggc aattcaaaag
    10321 ctaccggtcc tgttcgccat tgaccgcgcg ggcattgttg gtgctgacgg tcaaactcac
    10381 cagggcgctt ttgatctctc ttacctgcgc tgcataccag aaatggtcat tatgaccccg
    10441 agcgatgaaa acgaatgtcg ccagatgctc tataccggct atcactataa cgatggcccg
    10501 tccgcggtgc gctacccgcg cggcaacgcg gttggcgtgg aactgacgcc gctggaaaaa
    10561 ctaccaattg gcaaaggcat tgtgaagcgt cgtggcgaga aactggtgat ccttaacttt
    10621 ggtacgctga tgccagaaac ggccaaagtc gccgaatcgc tgaacgctac gctggtcgat
    10681 atgcgttttg tgaaaccact tgatgaagcg ttaattctgg aaatggccgc cagccatgaa
    10741 gcgctggtca ccgtagaaga aaacgccatt atgggcggcg caggtagcgg agtgaacgaa
    10801 gtgctgatgg cccatcgtaa accagtaccc gtgctgaaca ttggcctgcc ggacttcttt
    10861 attccgcaag gaactcagga agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg
    10921 gaagccaaaa tcaaggcctg gctggcataa tccctactcc actcctgcta tgcttaagaa
    10981 attattcatg gcaggagtgg cagcatgcaa tacaacccct taggaaaaac cgaccttcgc
    11041 gtttcccgac tttgcctcgg ctgtatgacc tttggcgagc cagatcgcgg taatcacgca
    11101 tggacactgc cggaagagag cagccgtccc ataattaaac gcgcgctgga aggtggcata
    11161 aatttctttg ataccgccaa tagctattcc gatggtagca gcgaagagat cgttggtcgc
    11221 gcactgcggg atttcgcccg tcgtgaagac gtagtcgttg ccaccaaagt gttccatcgc
    11281 gttggtgatt taccggaagg attatcccgt gcacaaattt tgcgctctat caacgacagc
    11341 ctgcgacgtc tcggcatgga ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac
    11401 acgccgatcg aagagacgct ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt
    11461 tatatcggcg cgtcatccat gcacgcttcg cagtttgctc aggcgctgga actacaaaaa
    11521 cagcactgct gggcgcagtt tgtcagtatg caggatcact ataatctgat ttatcgcgaa
    11581 gaagagcgcg agatgctgcc tctgtgttat caggagggcg tggcggtgat tccgtggagc
    11641 ccgctggcgc gggggcgact gacgcgtcca tggggagaaa ctaccgcacg actggtgtct
    11701 gatgaggtgg ggaaaaatct ctataaagaa agcgatgaaa atgacgcgca gatcgcagag
    11761 cggttaacgg gcgtcagtga agaactcggt gcaacacgag cacaagttgc gctggcctgg
    11821 ttgttaagta aaccgggcat tgccgcaccg attatcggaa cttcgcggga agaacagctt
    11881 gatgagctgc tgaacgcggt ggatatcact ttgaagccat aacagattgc cgagctggaa
    11941 acgccgtata aaccgcatgc ggtcgtagga tttaaataag agtccaggcc tgatgagacg
    12001 tgacaagcgt cacatcaggc atcggtgcac aactacgaca gaatacccag cggccagtga
    12061 tgaccgataa agtacaggat gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg
    12121 atccccatgc cgccatgtac attgcgatca aaccagcgga tcggccacgg cttccacata
    12181 tccagaatac ggaaaatcac aaacccggcg gcaacccact gccagtcatt ggtcggcagc
    12241 gccatgagcg tgatccacat accaataaat tcgtcccaga caatgctgcc gtgatcgtgc
    12301 acgcccatat ctttcgccgt ttgatgacaa agatagacgc cgatacagat ccccaacatc
    12361 accaccagcg agtagagctg ccagggtaaa aaggtcatca gataccagaa agggatcgcc
    12421 gccagcgatc ccatcgagcc aggaacgatt gggcttaatc cacttccgaa tccgacagca
    12481 agcagatgcc acggattact catcttcagg cgacttttcg cgacatcttt atggcgtgac
    12541 aaaatggtca tatcctttcc agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag
    12601 cccttcgata tcggcggtca tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag
    12661 agcaacatcc agcgcgccac ggttcagttc cggcaccgtg aaacacaact cgtaatcttc
    12721 accgccagag agcgcccagc gcagcgcctg ttccggttca acatggcgag aaagcgcatc
    12781 agaaaacggc agcaatgcca ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg
    12841 cccgagatcg gaaatcagac cgtcagagag atcgatagct gaatttgcca gatcgcgcag
    12901 tgcctgcccc tgtaaaatac gcggcgatgg acggagatga cgtttgatca agtagtcagc
    12961 atctttagca tcggcaacct gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc
    13021 gcccggtgta ccggtcacat agatccagtc acccggtttc gccccagagc gcgttaaggc
    13081 tcgtcccatc ggaacaaagc cgtggatacc caacgtcatt gataatggcc cacgcgtggt
    13141 atcgccgcca atgagttgca tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa
    13201 ggactcaagc cacgcttcgt ctacgtccgg taaggttaat gccagcgtca gccaggccgg
    13261 atcggccccc atcgctgcca gatcgcttag gttcaccgcc agtgctttat aagccagatc
    13321 agcaggatcg atatcaggga ggaaatggtt gcccgccacc agcgtatcag tgctgatcgc
    13381 cagggtctgt ttctcgggga tattgagaag agcgcaatcg tcgccgatgc ccagttcgac
    13441 atcaagacga gaacttctta cacggtcaaa ataacgggca atcagggaga actcgccaca
    13501 tgccatacgt tatgcctcag caggtaaaaa agaaaaggcc ggcgatcatg gaacagtctt
    13561 ccgtgaatct accggcctgg atattacttt ttgttagggc gaatcacagg tgctgcttta
    13621 tcgagtacgc cgttgacgaa cttatggctg tcttctgcgc cgaacgattt cgccagttcg
    13681 atcgcttcgt taatggccac tttgtatggc acatcactac gtttagacag ttcgtacagc
    13741 gcaatgcgca gtactgcttt ttctacctgg cccagttctt ccagcaggcg ggacaggtag
    13801 ggcttcatca gtccgtcgag gtatgcggtg ttagtcgcca ccccggccag cagctcacgg
    13861 aagtacagga cgtcaacgtc ttttacatcc tgttcagcca ggaactggta ttcaacatca
    13921 gcgatgtcgt tctgggacaa ctgccaggag tagagcgcct ggacggcaca ctcacgagcg
    13981 cggcgacgag cagcaggttt cacggatttc cccttactaa tttcaggcct tgatggcttt
    14041 caatacatta atcatttcaa gcgcggtcag tgcagcttct gcacctttgt tgccagcttt
    14101 ggtgccagca cgttcgatcg cttgttcaat gctttcagtg gtcagaaccc caaaagcaac
    14161 cggaatttcg ctgtcctggg caacatgcgc caggccgttg cttgcaccac cagcgacata
    14221 ttcaaagtgg gcagtgccac cacgaataac cgtacccagc gcaatcaccg cgtcgtattt
    14281 accggtttta gccagtgcac ccgccgccag cggcagctca taggcaccag gcacccaaac
    14341 aacggtaatg ttttcatctt ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc
    14401 cagcaggctg tcattgataa agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc
    14461 cggggtagca acgttagctt caataatgtt cattttcttc cttcgggttc gagtatggcc
    14521 ccgcaggggg gcggatttta gcataatatt tcgtgcgctg cttccctttc gagccgggag
    14581 atcatgcacc cactaaatgc aggcaaacat ccgggcctac atgacgtatc tctttgaatt
    14641 taaattgggg ggcgtcggct aatttctcaa gccctggcag cgagcataat ccacgggcgt
    14701 cgctgcctaa tagtttaggt gcgatataga caatcagctc atcgactaaa cccgcctgta
    14761 gcaatacgcc agcgagcgtt ggccccgctt ccacccagat gctgttaatc tgctgtttac
    14821 ccagttgcat catcagtaca accagatcca gatgaccttt atgctctgga atcagcaagg
    14881 tacgcaccgt ttccggccac tcacgagaat cttcctgcgt acgcgcgaac caggtttcgc
    14941 cgggctgctg cacaatgcga tgttccggcg tcacgcgatt ttggctatca atcacaatac
    15001 gtactggctg gcggagattt tgttgcgggt agagcgcctg agtttgttca tccagttcag
    15061 accaacgcac tgttaaggct ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg
    15121 catgactttg tgcgcgctgt cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact
    15181 ggctttcgcc gctcgccatc gccgtgcggc catcaagcga tgcgccaagt tttaactgaa
    15241 tataaggaaa gccggtgcgc atccgcttga gaaagccttt attcaattgc tcggcttcac
    15301 tcatcatcag cccgtggctg acgtcaatgc cagcctgttg cagacggtaa agtccacgcc
    15361 cagcgacctg cgggttagga tcttgcatcg ctgcgaccac gcgcgctacg ccagcggcga
    15421 tgagtgcgtc acagcacggt ggcgtacggc catgatggct acagggttcg agtgtcacat
    15481 acgccgttgc gcctttggct ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg
    15541 gttcacccgc tcgttggtgg taaccttcac cgacaatttc gccatctttg acaatgacgc
    15601 acccgacatt cgggttgggg tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc
    15661 gcgccatgta atactcgtcc tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa
    15721 attctttgat atcttcgaaa ctgcggtaga cagaggcaaa acggatatag gcgactttat
    15781 cgagcttttt caattgctcc atcaccagat tgccaatcat cttgctcggt acttcgcgct
    15841 caccggtggc gcgcagctgc gatttaatat gattgattgc catttcaacg taatcggaac
    15901 tcaccggacg tttttccagc gcccgcagca ttccgctacg caatttctct tcattaaacg
    15961 gttcacgcac gtcgttgctt tttacaacac gcggcataac cagctccgcc acttcaaagg
    16021 tggtgaaacg ttcattacac accagacact gccgacggcg gcgtacggat gaaccctcgc
    16081 ccacgagacg agagtcaatt accttagtgt ccacggcgaa acagaatggg caatgcatac
    16141 ggcgtcctga ccaggtggtt aacggaaatc tattttaccc tgaactgacg atacaacaaa
    16201 ggcgcaacgc ttttgagata aaggatcgat gctttcgctg cttttaccct ctaccattag
    16261 gctaattgcg acgagaaagg gacgacctat gaatactaac gtttttcgac tgctcctgct
    16321 gggaagcctg ttcagcctta gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca
    16381 cagcgtcagc acgctgaacc aggagatgac gcaactcaat caagaaacag tcaaaatcac
    16441 tcagcaaaac aggctgaatg caaaatccag cagtggggtt taccttctgc ctggagcgaa
    16501 aacaccggca agactggaaa gccagatcgg tactttacgt atgtcgctgg tgaatattac
    16561 gcctgataca gatggcacca cccttacact acgtattcag ggtgagtcga atgatccctt
    16621 gcccgcattc agcggaactg ttgaatatgg gcaaattcag ggaacaatag acaactttca
    16681 ggaaatcaat gtgcagaatc aattgattaa tgcccctgcc agcgtcctcg cccccagcga
    16741 tgttgatatt ccgttacaat taaaggggat ttctgtggat cagttaggct ttgtacgtat
    16801 ccacgacatt caacctgtta tgcagtaaac gtatttcggg acgattttgt gcgtcccgca
    16861 acatcttccc cgtcattttg ttactctgct tacatcacct ggattgatag taaaagtttg
    16921 caacaagggc gagagtcagt acaatccccg cccgaatgtg tgtaaacgtg aacgcaatcg
    16981 attacgtaaa tgatagaact gtgaaacgaa acatattttt gtgagcaatg atttttataa
    17041 taggctcctc tgtatacgaa atatttagaa acgcaatttg cgcctttttc actcccgcaa
    17101 gggattttca aacagtggca tacatatgaa aaaaacatta ctggcagccg gtgcggtact
    17161 ggcgctctct tcgtctttta ctgtcaacgc agctgaaaac gacaaaccgc agtatctttc
    17221 cgactggtgg caccagagcg ttaacgttgt cggaagctat cacacccgtt tcggaccgca
    17281 gatccgcaac gatacctacc ttgagtacga agcattcgct aaaaaagact ggttcgactt
    17341 ctatggttat gcggatgcgc cggtattctt cggcggtaac tccgatgcta aaggtatctg
    17401 gaaccacggt tctccgctgt ttatggaaat cgaaccacgt ttctccatcg acaagctgac
    17461 caatactgac cttagcttcg gtccgttcaa agagtggtac ttcgcgaaca actacattta
    17521 cgacatgggt cgtaataaag atggtcgcca gagcacctgg tacatgggtc tgggtaccga
    17581 tatcgacact ggcctgccga tgagcctgtc catgaacgtc tatgcgaaat accagtggca
    17641 gaactatggc gcagcgaacg aaaacgagtg ggacggttac cgtttcaaaa ttaaatactt
    17701 tgtgccgatt accgatctgt ggggcggtca gctgagctac atcggcttca ccaacttcga
    17761 ctggggttcc gatttagggg atgacagcgg taacgcaatc aacggtatta agacccgtac
    17821 taataactct atcgcttcca gccatattct ggctctgaac tacgatcact ggcactactc
    17881 tgtcgtagct cgttactggc acgacggtgg tcagtggaac gacgatgcag aactgaactt
    17941 cggcaacggc aacttcaacg ttcgctctac cggctggggt ggttacctgg tagtaggtta
    18001 caacttctga ttatgaaaat gccgggattt attcccggca tttctgattg ttaacaacgt
    18061 gtgctttgtt catgccggat gcggcgtaaa cgctttgtcc ggcccacaaa agcacgtaaa
    18121 ttcaatatat tgctgaaata acgtaggcct gataagcgta gcgcatcagg caattttacg
    18181 ttttaatcac ttcttcttgt tcatcatcgc tttcaggtca gcgaatgggt tgtacttcgc
    18241 ttcaccgaca tctttctgcg catcttcccc tgcgataacg gtcgtaccat actgatccgc
    18301 ttcggtatat ttcgaatgct catgatcgtg gcaatagaga cacaacaatt cccagttact
    18361 gccatcttcc gggttattgg tatggtcgtg atcaatgtgg tgaacggtaa gttcacgcag
    18421 gttagaataa acaaactcgc gggaacagcg accgcagacc cacggataga tttttaatgc
    18481 tttttcgcga tagccgcttt ctaaccgtgc atagtttttt gggatgatag ccatgaaaag
    18541 ttacctggat gaaatgtagg gttacgcgca gtttcccaag atgcggggat tttcgcaatc
    18601 tccatacatc aaaaaaaatc ccgatcttct gaccgggatt ttcaacatca acgggaactt
    18661 gattaaggca gaattgacgg ctgatccgcc ccttcttttt ccactttctg ctgcaacatg
    18721 tgttcgcgct tcatacccag tttcagagcc aacgcagatg ctacgtagat ggaagatgca
    18781 gtgccgatgg aaacaccgat aagcatggtc agcgagaagc cttccagtac cggaccaccg
    18841 aagagataca gcatcaggat aaccatcaag gtagtaccgg atgtgatcaa ggtacggtgc
    18901 agcgtctggg tcaaggacac gttaaagatt tcgtaaggcg taccgcgacg gatcttgcgg
    18961 aagttttcac gaattcggtc cgataccacg atactgtcgt taagcgagta accgataacc
    19021 gacatcaacg atgccacaat ggtcaggtca atctcgatat ggaataacga caaaataccc
    19081 agcgtaatga taacgtcgtg cgtcagcgca ataaccaccc ctgccgccag tcgccactca
    19141 aagcggaaac ctacgtacac gaggatagac agcagcgctg ccatcaacgc catcgcaccg
    19201 gtttgcgcaa ggtcagcccc cacgctcgga ccgacgaact caatacgctt cactgctgca
    19261 ttctgattgg tggattcgtt aatcaccttc agaacttggc tgcccagcac ctgaccgccg
    19321 gtttcgcctt cagcaggcgg catacggacc atgatgtcgt ggctgctacc aaagttttgc
    19381 agcattggct cttcaaaacc ggctttttgc aatgcatcac gcattacgtc aatttcagcc
    19441 ggtttttcga gcgtaatttc gataaccgta ccaccggtga aatccagccc ccagttaaag
    19501 ccgcgcacgc ccataataac gatagcagcg attaacagca gaccagagat gccgaaagcc
    19561 cagtagtccc agcgcataaa gtcatagact ttacggccgt ggtttagttg ttcaacagta
    19621 tattcctgtg ccacatcgca ctcctcagat tgacagcttc ttgacgcgct tgccgccata
    19681 tagcaggttt acgatggcac gcgtaccgac aatcgcggta aacatcgacg tcgccacacc
    19741 gataccggta gtaatcgcga accctttaat tgccccggta cccactgcgt acaggatgat
    19801 gactttaatc agcgtggtga tgttcgcatc gaagatagaa ctgaatgcgc cacgataacc
    19861 ttcatcaatt gcctgttgaa cagtacgtcc gttgctcaac tcttctttaa tacgttcgtt
    19921 gatcagtacg ttcgcatcga ccgctaccgc aagggttaag acgatacccg cgatacctgg
    19981 catactcagt gttgcgcctg gcaacagcga cataataccg acgatcaaga tcaagttggc
    20041 aatcagagca ctggtcgcaa tcagaccaaa cttcttatag aagatgatca tgaacagaat
    20101 agaaaccagc agaccggcaa ggcaagcctc cagcccctgc tcaatgttct gcatacccag
    20161 ggtcgggcca attgtacgtt cttcaacaat ctgaatcggc gcgatcaacg caccggcacg
    20221 cagcagcagt gacagctgac gggcttcgtt cgggttgttg atgccggtga tacggaagct
    20281 gttacccaga cgagactgga tgttggcgat gttaatcacc tcttcctgtt tcaccagaac
    20341 cgcacgacca tttgcatctt tcttaccgct gtctttgtac tccacaaaca gggttgccat
    20401 cggtttgccg atgttgtcct tggtgaagtt agacataatg ttgccaccag cgctatcgag
    20461 cgagatgtta acctgcggct ggttgtattc gtcctggctg gaagtggagt cggtgatatg
    20521 gtcaccagtc agaattacgc gtttgtacag cacaaccggc tgaccttcgc gggtctgttt
    20581 cacttcagag tcgcccggta cgcgaccgga tgccgcagcg gcctgatcaa cattggtgtt
    20641 taccagacgg aattccagcg ttgccgtcgc gcccagaatc tctttcgcac gcgcagtgtc
    20701 ctgaataccg ggcagttcaa caacgatacg gtcagcaccc tgacgctgaa ccaccggctc
    20761 cgccacgcca agttggttta cacggttacg caggatatta atgttctgct gcaccgcata
    20821 ttcacgcgct tcactcagac gagcatcgct cattaccgca cgcagctggt tgctgccctg
    20881 gctgctaatc accaggtccg gatggcgctt gctcagatac gcaatggctt catcacgagc
    20941 tttagcatcg cggaaagtga tgctcaggcc gtagttgttt tctttacgaa cagtggtata
    21001 cgggatgccc ttttcgcgca ggtcactgcg taggctatcg atattttgtt cctggagttt
    21061 gccaagcgcg gtgtccatat ccacttccat caggaagtga acgccgccac gcaggtcaag
    21121 gccgagcttc atcggctcag catgaatagc tgccagccag cgcggcgttg ccggggcaag
    21181 gttaagcgcc acgacgtatt tgtcacccat aacgcccatt aatgcttcac gagcgcgcaa
    21241 ctgggtgtca gtggagtcga agcgcgcaag aatagcgccc tcttccagtg ccacagactt
    21301 agcagttatt ttttcttctt gtaacgtttt ctggacctgg atcagcgttt gctcactggc
    21361 ggcgactccg cgcgcaccag tgatctgaac agccggatcc tcaccaaaca ggttgggaag
    21421 cgcatacagc agaccgatga caatcaccac gatcagcatg acgtacttcc acaaaggata
    21481 acggtttaac acggcaattc ccttagggaa aaattttaat tacagcgcct tcatggtgcc
    21541 tttcggcagg acggcagcta cgaagtcacg tttaataact acttcagtgg tgtcattcag
    21601 cgcgatagca atgtagccgt tttccgctac tttggttacg cgaccaacca ggccaccgtt
    21661 cgtcagaact tcatcacctt tggcaatgga gtccatcagc tttttgtgtt ctttggtgcg
    21721 cttctgctgt ggacgcagga tcatgaaata gaaaatcaga ccgaatacca ccagcatcaa
    21781 aatcaaagac atcgggctac cttgcgccgg tgcacccgtt gccgctaccg catcagaaat
    21841 aaaaaagctc attaaatttc cctcattatt aatattaatc aacgttcaaa ggtggtactt
    21901 ctcgcccctg acgctggtaa aaatcagtta cgaagctctc taatttaccc tcttcaatag
    21961 ccttgcgtaa acccgccatc aaacgctggt agtaacgaag gttatgaatg gtgttgagtc
    22021 gcgcgcctaa tatttcgttg caacggtcga gatgatgcaa gtaagcgcgt gaataattgc
    22081 gacaggtgta gcaatcacac tcaggatcga gtgggccagt atcgctctta tatttcgcat
    22141 tgcggatttt caccacgcca tcggtcacga acaaatgacc atttcgggcg ttgcgggttg
    22201 gcattacgca gtcaaacata tcgataccgc gacgtacgcc ttcaaccagg tcttccggtt
    22261 taccaacgcc catcaggtaa cgcggtttgt ctgccggaat ttgcggacac acatgctcca
    22321 gaatacggtg catatctgct ttcggctcac ccacagccag accgccgaca gcgtagccat
    22381 caaagccgat atctaccaga cctttaacag atatatcacg taaatcttcg taaatactgc
    22441 cctgaattat accaaataac gcatttttgt taccaagact gtcaaaacgc tcacggctac
    22501 gcttcgccca acgcagagac atctccatag agcgttttgc gtaatcccag tcagcaggat
    22561 acggcgtaca ctcatcaaag atcatgacga tatccgaacc aagatcgtac tgaatctcca
    22621 tcgacttttc cgggtcgagg aaaatcgggt cgccgttgat cgggttacgg aagtgaacgc
    22681 cctgttcggt gattttacga atatcaccaa ggctgaagac ctggaagccg ccggagtcgg
    22741 taagaatcgg tcctttccac tgcataaaat cgtgcagatc gccgtgcagt ttcatgattt
    22801 cctggcccgg gcgcagccac aggtggaagg tgttgccgag gataatttgc gcgccagtgg
    22861 cttcaacttc ttccggcgtc atccctttta cggtgccgta ggtgccaaca ggcataaaac
    22921 aaggcgtttc cactacgcca cgatcaaaga ccaggcggcc acggcgtgcg cgaccgtcgg
    22981 tggtgtccag ttcaaatttc attttttctc cactacgtca gaaaaacagt ccaacgttta
    23041 aaccagcgcc gcggaattac tccccgacgc gctcattaat tgcctgcgga ttgtacgtga
    23101 taaacatcgc atcaccgtaa ctaaaaaagc gatatttctc ttctaccgcc gctttatagg
    23161 cattcatggt gtgttgataa ccggcaaagg ccgaaaccag cataatcagc gtcgactctg
    23221 gcaagtggaa gttcgtcacc agcgcatcga ccactttgta ctggaagccc ggatagataa
    23281 agatttgggt atcgtcaaag aacggttcga taagatcgtt ttttgccgcc tgtgccgcgc
    23341 tttccagcga acgtactgaa gtggtgccaa ccgcaatcac ccggttaccg cgcgctttcg
    23401 ccgccagtac cgcgtctacc acatcctgcg gtacttcagc gtattccgag tgcatgatgt
    23461 gatcttcaat ggtgtcgacg cgcaccggct ggaaggtgcc cgcaccaacg tgcaacgtca
    23521 caaacgccat ctccacgcct ttggcgcgta atttttccag caaaggctcg tcaaaatgca
    23581 gaccagcggt cggggctgca accgcgcccg gtttttcgct ataaacggtt tgataaagtt
    23641 cgcggtcagc gtcttcgtcc ggacgatcga tatacggcgg cagcggcata tggccgatgc
    23701 tgttgaggat atccagtacc gagcgttcat cattaaattc gacttcaaac agtgcgccgt
    23761 ggcgcgcggt cattgttgcg ttgatacttt cgtcatcgcc cagcaacagt tctgcgccag
    23821 gttttggcgc tttcgaggcg cgaatatgcg caagaatgcg tttgtcgtcg agcatccgct
    23881 caaccagcac ttcaatcttg ccgccgctgg ctttacgccc aaacagacgc gccgggatca
    23941 cgcgggtatt attaaaaacc agaagatcgc cggggttgag cttatcgagt aaatcggtga
    24001 aagtaccgtg cgtcagcgcg cccgtcggcc cgtccagcga cagtaaacga cagctactgc
    24061 gttcaggcat gggatagtgg gcaatcaggg attcgggcaa ttcaaaggag aaatcggtaa
    24121 cgcgcatgac actgactcag actaaaataa gagtcgggta gtctagtgcc ggggcgctct
    24181 ccctgcaacc tttacccccc tttggataga atataatcga tgaatttttt agctcacctg
    24241 catttagccc atctcgcgga aagctcgctt tctggcaatt tactggctga tttcgtacgc
    24301 ggaaatcctg aagaaagttt tccgcccgac gtcgtggctg gcattcatat gcatcgacgt
    24361 attgactgac aatctgccgg aagtccgcga agcacaggag tggtttcgta gtgaaacgcg
    24421 ccgcgttgcg cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc
    24481 gcagctgtcg ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat
    24541 gacgattttg ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga
    24601 gcagtggctg gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc
    24661 aagccgccgc ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta
    24721 tgacgccctc gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca
    24781 caaggcgtta taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga
    24841 atcccgtcag tcagttgccc atgcccttct ttgcattgcc attgcacggc attgagaaac
    24901 ggtgacgcgg gtagcaccac ttcactggcc tcgccacggt tgattgccac cagtacacgt
    24961 tgctgattca gcacgcggac aaataccact acgttatctt ccgcatacag cacctgacag
    25021 ccgccacgac gcagcgcctg acttttctta cgcagcacaa tcattcgccg gtacagcgcg
    25081 aataacgccg tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg
    25141 tttttaccat ccagtcccac ttcatcaccg taataaatgc acggtacacc aggccaggtg
    25201 aacagccaga ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat
    25261 cgcgcagtat cgtggctatc gagctgatta aacatacgta attgttgttg atgagaaagc
    25321 cctgcgcggt aattatccat ccaggccata caggtttggg cgtcaatttg ctgcggatca
    25381 taagagatgt cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc
    25441 atggcagcat cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg
    25501 acaatatacg cttccggctg ggtttctttc gccgcttcgg tgatcccggc aacgtgctgc
    25561 atattattgc gcgccccacc cgcctccccc agcatatgca ccacatccag ccgccagccg
    25621 tccatattcc acggcgcttt cagccagtgg cggacaatac tgtcttcccc gcgataaatt
    25681 tcattcacca gactttccga ctgataatcc agcttcgtca agctggcata gccaagccag
    25741 tcgagcgccg tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagagttg
    25801 tgacaagctc cccccgtgcc aagattgtgc ctgtcaaacc aggcatggga atcgccactg
    25861 tggttaaaca cgccgtccag caccagccgc attcccagct gctgcgtatt gtgtcgcaaa
    25921 cgcagcaacg ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg
    25981 tatttatgta cgctgggagc tttaaacacc ggattgagat acagcgctgt cacgccaagc
    26041 tttttcagat acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat
    26101 ccgcccgcct gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct
    26161 gcatgatggt aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga
    26221 tcagggaaga tctgataaaa aatctgatcc gcagcccatt gtgggccgat atcaggtaca
    26281 tcgacggcaa actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac
    26341 caacgctggc gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg
    26401 gagagatcaa tcgccgcacg ccatgcggtg acgcctggct gcggctgact gcgctgctta
    26461 tgcattggta ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt
    26521 gggtcttcgc ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat
    26581 gggggcaccg gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat
    26641 caccttgcca tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg
    26701 acgggggcgg ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag
    26761 aacgcaagta ttattggttt tcagccagct gacgatcgtg gcggcgttta aacatccagc
    26821 caatcaacag cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt
    26881 gataaccaat caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta
    26941 ccccacccgg cactttaaat ttcagcgcct taacttcttc tggcggcaaa cggcgacgga
    27001 aggcgatttg cgacagcagg atcataatcc acacccatac cgtggcgaag gttgccagcg
    27061 aagcgatcac caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca
    27121 gcgcggtagt catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga
    27181 aaattttcgg tgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaata
    27241 cgtcactgtt aattgccgac agcgaagcgg tcagcacaac aaagttaaga atgctggcgg
    27301 caaaggtaat gcccatatgc tggaacgtca gtacgaacgg gctaccggca gtgccaacct
    27361 gattccacgg gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac
    27421 gcatcggcac ggagttaatc gcacgcggaa tcgatttctc aggatctttc gcttcaccgg
    27481 cggtaatccc gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatta
    27541 ccatgccaag ccagccgtta ctgaagaagc cgccgttgct ccacaggtta tgaataccgg
    27601 tcggttgccc gccgttgcca atcccccaga tgatgatgcc gaaaccggcg acaatcatga
    27661 tgatgatggt ggcgacttta aagaacgaga accagaattc cagctcaccg aagaccttca
    27721 cgctcatcag gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg
    27781 gcaccgtcgg gaacaagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga
    27841 caataaggat ttcaaaacag taggtccatc cggtaatgta acctgccagc gggccaaggt
    27901 tttcctgcgc ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca
    27961 gcgcacgcat aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg
    28021 gaccggccat tttgatggcg tctgccgaac cgtaaaacag cccggtgcca attgctgaac
    28081 ccagtgccat aaagcgtatg tggcgggtac ttagcccacg ctttagctta ttcttacttt
    28141 ccatcaattc ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt
    28201 aaaacaaatg ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat
    28261 cccagataat ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt
    28321 cggccagcgg taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga
    28381 tcccgtcgag aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg
    28441 aattatgcca ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa
    28501 tagcggtcag caccggtacg gagatctgga tcagctggct taagccgagg ttagacacca
    28561 ccatcgagaa gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg
    28621 caaagaattc tgcacaagca caggtcaggc caaccgccgt gaccagacag gcgatgaaga
    28681 ttaacgccgc cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca
    28741 gaatagcagc gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacaga
    28801 acagcgccag gtacagcaaa gtcagaccaa cacccgccat caggccagcc cagacggtat
    28861 aacgggtcag cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga
    28921 taccaaacac cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag
    28981 aaaatgcagc gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa
    29041 tcgcgacaac agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca
    29101 cggtatccag cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca
    29161 ggctgtagat aaacagcggc agcgcggaat cacccgtcag cggcgcaatc cccacttcaa
    29221 aggaaacggt agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg
    29281 ttgccagcag tacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc
    29341 caacttttgc cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc
    29401 cgaatgccgc agtccagacg tgttcgcctg cctgtaagcc gaccattgga gggaaaataa
    29461 tgttacctgc gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg
    29521 atcttaattg atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt
    29581 tttgtgccaa tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac
    29641 tcccaaccag gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg
    29701 acagcacctg aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa
    29761 accagaggta tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa
    29821 agacatggct aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat
    29881 tttgactgca aagaaaataa gccagcatcg gctggcttat ggcaataaaa gatgacaaag
    29941 gcggattaat cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt
    30001 ccttttccta ctgtactctc aatattcagg cgactttcgt gatgattcac cgcatgtttc
    30061 acgatcgcta accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga
    30121 taaaaacgct cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca
    30181 acgctaaatt cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttcc
    30241 ggcgtatgat tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg
    30301 ttgccagaca ccttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc
    30361 agagtctgag cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg
    30421 agcaattgcg tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct
    30481 tccatccgct gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc
    30541 agcggctgct catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac
    30601 tcatggctca cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg
    30661 acatcacgcg ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga
    30721 tgccgcccgg tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat
    30781 tgcgtaaact ccgggtaacg cagtaggtta aggatgttct gcccgttatc ttccggccag
    30841 cgcaaaccaa gaatttgttg cgccagaccg ttacaccaga aaataccgcc ctcttccgtg
    30901 gtcagcacca ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg
    30961 cccagttcac ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc
    31021 ggttcccagc taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat
    31081 gaaaggcgca ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa
    31141 aaccagggca ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc
    31201 agctccagca ccagcctttt ccacgacagc cgttccagca cgtaagatac tccagttaag
    31261 aaatcataag ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga
    31321 tatcctgtac cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta
    31381 cgcaggcgac gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag
    31441 acgtggttta acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc
    31501 agcagtttaa attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga
    31561 gatgtcgggt tgagacttaa tccctgcatc ttaatcacct cttccaccgc cattggcgaa
    31621 atacggcgca ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa tggcttggtg
    31681 atatagtcat ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg
    31741 gtcaacatca ccactggaat atcccgggtc atcgactcgc gcttgaggtg tttgatgaac
    31801 tggataccgg agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca
    31861 ttcagttgat tcacagcact gtcataatct tccgcttcga ccggctgaaa gccattttgt
    31921 tcgagcacga agcagaccat ttcgcgaatt ggagcttcat cttctacgac cagaatacgt
    31981 ctcgccatga tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat
    32041 gcgtcagatt tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt
    32101 tccgtggcgc agaaaaaagc aaatggcaca tctgtttggg tataatcgcg accatgcttt
    32161 ttcgccaggg aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact
    32221 tctacagtaa aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacag
    32281 cacaaaccca tcaggtggat gcgattattg ttgctggtga tgttttcgat accggctcgc
    32341 cgcccagtta cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct
    32401 gtcatctggt ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg
    32461 atatcatggc gttcctcaat acaaccgtgg tcgccagcgc cggacatgcg ccgcaaatct
    32521 tgcctcgtcg cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgcccgc
    32581 gtgacattat ttccagccag gcggggctta acggtattga aaaacagcag catttactgg
    32641 cagcgattac cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc
    32701 agcctctgcc catcatcgcc acgggacatt taacgaccgt gggttccagt aaaagtgacg
    32761 ccgtgcgtga catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag
    32821 ccgactacat cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg
    32881 ttcgctattg cggttccccc actccactga gttttgatga atgcggtaag agtaaatatg
    32941 tccatctggt gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg
    33001 taactcaacc catggcggtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac
    33061 agtggcgcga tgtatcgcag gagccacctg tctggctgga tatcgaaatc actactgatg
    33121 agtatctgca tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag
    33181 tattgctggt acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg
    33241 aaaccctcag cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagacc
    33301 tggatgaatc gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cgtaccctcg
    33361 ccggagaaca cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa
    33421 aggcgaatgg aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgccat
    33481 taccggccca accggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta
    33541 tcacgaaact ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga
    33601 taccgctgaa tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt atcgcgcgtt
    33661 ctggagccag aatcgggcgc gtaaccagcc ggacggtaat ttgcaggtgc ctcgcgtaga
    33721 gctggcgcgc tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata agctggaact
    33781 gacagcgacg ttaaccgggc tggattacgg gcgcttcacc cgttcgatgc tgctttcgca
    33841 ggggcaattt gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga
    33901 gttaaccggc actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc
    33961 ggcccgcact gagctggaga agctgcaagc gcaggccagc ggcgtcacgt tgctcacgcc
    34021 ggaacaagtg caatcgctga cagcgagttt gcaggtactt actgacgaag aaaaacagtt
    34081 aattaccgcg cagcagcaag aacaacaatc gctaaactgg ttaacgcgtc aggacgaatt
    34141 gcagcaagaa gccagccgcc gtcaacaggc cttgcaacag gcgttagccg aagaagaaaa
    34201 agcgcaacct caactggcgg cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca
    34261 ctgggaacgc atcgcagaac acagcgcggc gctggcgcat attcgccagc agattgaaga
    34321 agtaaatact cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc accacgcggc
    34381 gaagcagtca gcagaattac agcagcagca acaaagcctg aatacctggt tacaggaaca
    34441 cgaccgcttc cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca
    34501 aaccagcgat cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa
    34561 acttaatgcg cttgcggcga tcacgttgac gttaaccgcc gatgaagttg ctaccgccct
    34621 ggcgcaacat gccgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat
    34681 tgttccccaa caaaaacgtc tggcgcagtt aatggtcact atccagaatg tcactctgga
    34741 acagacgcaa cgtaatgtcg cacttaacga aatgcgccag cgttataaag aaaagacgca
    34801 gcaacttgcc gatgtgaaaa ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc
    34861 ccagcgtgca cagttacagg cgggtcagcc ttgtccactt tgtggttcca ccagccaccc
    34921 ggcggtcgag gcgtatcagg cgctggagcc tggtgttaat cagtctcgat tactggcgct
    34981 ggaaaacgaa gttaaaaagc tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc
    35041 attaacgaag cagcttcagc gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca
    35101 agcacttact caacaatggc aagccgtcac ggccagcctc aatatcacct tgcagccaca
    35161 ggacgatatt caaccgtggc tggatgcaca agatgagcac gaacgccagc tgcggttact
    35221 cagccaacga catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta
    35281 tcaacagcaa attgaacaac gtcagcagca gctcttaacg gcattggcgg gttatgcact
    35341 gacattgcca caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca
    35401 gagctggcag caacgccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac
    35461 accgattctg gaaacgttgc cgcaaagtga tgatctcccg cacagcgaag aaactgtggc
    35521 gctggataac tggcggcagg ttcatgaaca atgtctcgca ttacacagcc agcagcagac
    35581 gttacagcaa caggatgttc tggcggcgca aagtctgcaa aaagcccaag cgcagtttga
    35641 caccgcgcta caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctaatgga
    35701 tgaacaaaca ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca
    35761 ggcgcaaacg ctggtcactc agacagcaga aacgctggca cagcatcaac aacaccgacc
    35821 tgacgggttg gctctcactg tgacggtgga gcagattcag caagagttag cgcaaactca
    35881 ccaaaagttg cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc tgaagcagga
    35941 tgcagataac cgtcagcaac aacaaacctt aatgcagcaa attgctcaaa tgacgcagca
    36001 ggttgaggac tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg
    36061 caagtttgcc caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac
    36121 ccggctgcac gtgcgctatc tgttacagcg caaagccagc gaggcgctgg aagtcgaggt
    36181 tgttgatacc tggcaggcag atgcggtacg cgatacccgt accctttccg gcggcgaaag
    36241 tttcctcgtt agtctggcgc tggcgctggc gctttcggat ctggtcagcc ataaaacacg
    36301 tattgactcg ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac
    36361 cgcccttgat gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca
    36421 cgtagaagcg atgaaagagc gtattccggt gcagatcaaa gtgaaaaaga tcaacggcct
    36481 gggctacagc aaactggaaa gtacgtttgc agtgaaataa ctattcagca ggataatgaa
    36541 tacagagggg tgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta
    36601 ttggctacgg tgattggtag ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt
    36661 ttatctttgg ctctgggcac gtttggtttg gggatggccg aatttggcat tatgagcgtg
    36721 ctcacggagc tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg
    36781 tattatgcac tggtggtggt ggtcggtgcg ccaatcatcg cactcttttc cagccgctac
    36841 tcactcaaac atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc
    36901 acgctctctt cgtcttacct gatgctcgcc attggtcggc tggtatccgg ctttccgcat
    36961 ggcgcatttt ttggcgtcgg agcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc
    37021 accgccgccg tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg
    37081 ctgggaacgt atttaagtca ggaatgctgg cgttacacct ttttattgat cgctgttttt
    37141 aatattgcgg tgatggcatc ggtctatttt tgggtgccag atattcgcga cgaggcgaaa
    37201 ggaaatctgc gcgaacaatt tcactttttg cgcagcccgg ccccgtggtt aattttcgcc
    37261 gccacgatgt ttggcaacgc aggtgtgttt gcctggttca gctacgtaaa gccatacatg
    37321 atgtttattt ccggtttttc ggaaacggcg atgaccttta ttatgatgtt agttgggcta
    37381 gggatggtgc tgggaaatat gctaagtggc aggatttcag gacgttattc accactgcgc
    37441 attgcagcag tgactgactt tatcattgta ctggcactgc tgatgctctt tttctgcggc
    37501 ggcatgaaaa caacgtcgct tatttttgct tttatttgtt gcgcgggatt atttgccctt
    37561 tcagcacctc tgcaaatatt gttactgcaa aatgccaaag gaggagagtt attaggtgcc
    37621 gcaggtgggc aaatagcgtt taacctcggt agcgccgtcg gcgcatattg cggaggtatg
    37681 atgctgacgc tggggctggc atataattac gtggcgctgc ctgccgccct gctttcgttt
    37741 gctgcgatgt catcgttgct gctgtatggt cgctataagc gccagcaagc ggcggatagt
    37801 ccggtgctgg cgaaaccact ggggtagatt actgtcacgg tgcgcgttgc ttattgccgg
    37861 atgcggcgtg aacgccttat ccgccctacg cggttctggc acattttgta ggcctgataa
    37921 gacgcggcaa gcgtcgcatc aggcatgatg cgccaattgc ctacgttttt tactcttgtg
    37981 gccataacca cgcagcgccg cgtacgccgc tggaatcacc gtgcttcgcc ttactcaccg
    38041 gcgtttcaca ttcgccgccg aagacaaatt gtttaatcaa ctgcccaacc gtttgatata
    38101 aacggtctac attgctcatc ccgcccccca ggacaatcac atccggatcg agaatattca
    38161 cgacatgtgc cagcgatttt gccagccgca gctcgtagcg acgcaatgcc agttccgcta
    38221 ccggatcgct ttcttcaacc aggcggataa tttcactgcc tttcagcgca tgtccgctca
    38281 aacgacgata atccgtcgcg aatcccgtgc ccgaaataaa ggtttcaata caaccttgtt
    38341 taccgcaata acaagggact tcctcgcgat aacgcagttc gtcttcgtcc atccacggta
    38401 gcggattgtg tccccactca cctgccgtgc cattgccgcc gatatgcgtc cgcccattga
    38461 atgccacgcc cgcgccgcat cccgtgccga taatcacggc aaataccgtc tgcgctcccg
    38521 ctgccgcgcc atctactgct tctgaaaccg ccagacagtt ggcatcattt gccagccgca
    38581 cttcccgctg caacctcgcg cttaagtctt tatcgaatgg ctgaccgttg agccaggttg
    38641 aattgacatt cttcaccaca ccggtgtagg gcgaaatcga acctggaatg cccataccta
    38701 ccgttccgcg ctgccctgtc gcctgctccg ccatatcaac caacgtggcg atcgtttcaa
    38761 tagtctgccg gtaatcatca cgcggcgtgg gcagacgatg gcggtacaac tgctcccctg
    38821 catcgcccag tgcaatcact tcagttttgg tgccgcctaa atcgatacct atacgcactg
    38881 tactctcctt atttttttca atatcaatag cgtagagacg gacaaccgga ttggcaatgc
    38941 aaggccgccg acaattcgtt atcatgcccg ctaaatttaa cgacaaggcc gtggaaatta
    39001 tcatgctgtg gttcaaaaat ttaatggttt accgtcttag ccgcgagatt tcgctgcgtg
    39061 cagaagagat ggaaaaacag ctagcctcga tggcatttac cccatgcggc agccaggaca
    39121 tggcgaagat gggctgggtt cctccgatgg gatcgcacag cgatgcgtta acgcacgttg
    39181 ccaatggtca aattgttatc tgcgcgcgca aagaagaaaa aatcctcccg tctccggtga
    39241 ttaaacaggc gctggaagcg aaaatcgcca aactggaagc ggaacaggcg cgtaagctga
    39301 agaaaaccga aaaagattcg ctgaaagacg aagtgctgca ctctctgctg ccgcgtgctt
    39361 tcagccgttt tagccagaca atgatgtggc tcgacacggt taacggtttg attatggtgg
    39421 actgcgccag tgccaaaaaa gcggaagata cgctggcatt actgcgtaaa agcctggggt
    39481 cgttaccggt tgtaccattg agcatggaaa acccgattga actgacgctg accgaatggg
    39541 ttcgctccgg tagtgcggca cagggcttcc agctgcttga tgaagccgag ctgaaatcgt
    39601 tgctggaaga tggcggcgtg atccgcgcga agaaacaaga tctgaccagc gaagagatca
    39661 ccaatcacat tgaagccgga aaagtggtga ctaaactggc gctcgactgg cagcagcgca
    39721 ttcagtttgt gatgtgcgac gatggttcgc tcaagcgtct taagttctgc gacgagctgc
    39781 gcgatcaaaa cgaagatatc gaccgtgaag atttcgccca gcgttttgat gccgatttca
    39841 tcctgatgac tggtgaactg gcagcgttaa ttcaaaacct gattgaagga ttaggtggcg
    39901 aagcacaacg ttaattgctg attttccttt aatgccggat gcgacgcctg ccgcgtctta
    39961 tccggcgtac gaagccacac caggcatata attattcgct acggcgagca ataattttta
    40021 gcgcagcaat attatgcgtt ttacgccgta actgatctaa tcgcctcatc cctattcgcc
    40081 tgtcagcagc acgcagaata ttcactaacc cccatttatc gctggtaaat tgttttgtta
    40141 acagctccgc ttttggggta tcaatatatt cacgcaataa atcagaaatt gaacaaatat
    40201 ccgtcaggta aggataccag caagtttcac cgtgaaaatt acgcactcca gcgttacctg
    40261 caataaagtc tttcgggtaa tcccagatca cgtttttatc caacgtgatc caataacgcg
    40321 gtaaatcggt acttccacca ttttggctgc gcatgggata acgggtacag tgaatttgca
    40381 ggttgatcgt tggcgtcagt aagtcataga tctcccgctg taacttgctc catggacgtt
    40441 gtgtcattgt ttttcctcaa gccggtgacg cgcttcactg cgaattttcc gcgttgcccc
    40501 ggttttcact aaatcacgta actgcgaaag atcgggttgt tcgacaaata agagtaatga
    40561 tggatcctct tttaaccacg ccgttttcac atcggcagcc agctgtggat tattaatagc
    40621 cagcgaccga tcctgaggct cagtcaatgt cgttaacaac gacgccgggg tatgtgcgtt
    40681 gcgtaatgcc gtttggcgca aatgatcatt gcgactttgc agcaatgcac gccaccattc
    40741 atcatctgat ttatctttta tcgtagccag ttccgcaaaa aatacctgga gatcttcact
    40801 gatatttata tcaaccttga tgaattatga atatatcgga aattgaaaaa atcgcagcaa
    40861 aggaaatatt attatgggtt aaagagagga tatttcagaa aaatgccctc ccgttcaagg
    40921 ggaaggcgag gaattacaga tagcggcaca gataagaggt aggttcggca acttgaagat
    40981 gaaactcact gtgaccggga acattaaaca ccgaaccggc ttcatacact tgccagtcgg
    41041 tcgcgtcagg cagtaacaca ttcagcgcgc cactgattac cgtcatctct tccggctcag
    41101 cggtgctgaa ggtgtattcg ccttcaacca taacacccac gctggcgcga ccagtgctgc
    41161 tgctggaaaa gccgatcgat ttcactttgc cggaaaagta ctcattactt tgaagcataa
    41221 actggccctg tctggtagtt gaacatctac ttcactatag gggccagagg cgctggctgt
    41281 cacgcaaaat tacaccagta attccgcagc cagccgtgca atcaatacgt tagagagcaa
    41341 gacaggaaca tcgagctgtt tttgcagtaa atcgcgatga cgttggtgaa atcctaaaca
    41401 atccagcatg atgacatcag ccccttttgc cagtaattct ttcccagcat caatgatttt
    41461 ttgttctgaa tcatgaatgg ggttacccaa tgaaaatacc ggcgatttct gcaaaatttg
    41521 ccatttttgc gcctgaacgg gcagcatctc ctcaaccgga acgataaccc ccacctgatg
    41581 atcttcaaca atagaggaaa ccagtggagg caatattcgc gacggctcaa gaaagatcgt
    41641 attacgcgca gtcatactac taatgtttgc tgtactcatt aataaaatga cgtcataacc
    41701 ctgattatcg agcacttcaa ccacaccttg cagatctcgc tccactttac gacgcgaaac
    41761 atgggccagc tggttgtcat ttaataatgt gagaatggtg tcttcgcctg cttctggcgc
    41821 gtactccgcc atcacttctt cacgacttaa cttccccagc aggctatgat gggaaatatt
    41881 atcttcgtca atgtattccg tcaggagcgg caaaacctcc tgcataggta caatgccgat
    41941 ggtcaggatc gccaacgacg cactcattgt tcatccacct tacttttctt tcgtcgttaa
    42001 ttaccgggca agtgtgaagc accactgctg acacttactt gtgctacaaa tgacaaaaag
    42061 cgtagcagca gacgtgcacg gcataatgta aagattccaa atgattccag taatggaatg
    42121 ttattgttta atattctaat tattagaaaa acataaatta tgaaaaatgt gacgcagatc
    42181 ccttgtagtg gcactcagaa tcccttccga gcagtctgat gcggtcactc tttgtcaggg
    42241 tcctcatagc gttttttcgc atccatcgct tcctgagccg tcggatgttc actgatcaac
    42301 gagtctggtt taggatgatc ggctctgagt tggtaccagg ttaccgtctg tcctggcttt
    42361 cctttttcaa tcgtcactat atatgcttca cgaggataag gcggtttcgt tggcatcgtt
    42421 cttccttatt tcacgggatg aacgttaagt ataggcgctc taaaatcaac aattgatcgt
    42481 ctgtgccagg gcgctacgaa tttcagaaat cacctggctg ggttcgtttg ttgcgtcgat
    42541 gataatatgc gcaacttcgc gatatagcgc atcgcgttct tccagcactt cctgaacttc
    42601 ttcgctcagc ggttttcccg ttaaggttgg ccgtaaatct tcttccggtg cagcttgcag
    42661 tcggttaacc aggactgata ctggcgcaca caaataaacc acgatcccgt tattttgcat
    42721 gaagtgacga ttaaattccg tcagaataat gccgccgcct gtagcgataa cggtggatgc
    42781 cgcagttacc gcttccagcg ccgccgtttc tctggcgcga aatcccgccc actcttccct
    42841 ttcgacgatc tccgcgaccg tcatattgag ctgtgattgc aaccactgat cggtatcgac
    42901 aaaccgacgg ttaagcgaat cggcaagggc cattccgacc gttgttttac cacagccccg
    42961 aggcccgatc agaaaaagag gttgtgtcat cgtgggtttt ccccaatagg tcgcaatgcg
    43021 gcgaaagccg gtgtcatgag aatagcgatc ataccatcaa actagtacaa tttcgattgt
    43081 aaagaaaaaa ttccacttaa agtaaaaatt tcaatacatc ccttactata ccaataaata
    43141 ttcaagaatg aagtgtaaat aataaattac atttagccac gactacgttg cacttccagc
    43201 caccacttct ccagctccgc ggcaaaggcc tggcggtcac gttgtgaaag gctatctggt
    43261 ccgccggtct ggatcccact ggcacgtaag gtatccataa aatcgcgcat cgtcaggcgc
    43321 tcacgaatgg tcgctggcgt gtaacgttcg ccgcgcgaat taagcgccgc tgcgcctttc
    43381 tcgatggctt cagcagccaa aggtatatct gcggtgatca ccaaatcgcc cgcttcacac
    43441 tgccggacaa tttcgttatc ggcaacgtcg aaacctgccg cgacgcgcag cgtacgaata
    43501 aatcgcgatg gcggcacgcg taaactctgg tttgctacca gtaccagcgg catctgcata
    43561 cgttccgccg cgcgatacaa aatctcttta attacattgg gacacgcgtc ggcatccacc
    43621 caaattgtca taaagtcatc ctttgttagg taatcctcta ttgtgtcgcg cttttgcctt
    43681 ccggcatagt tctgtttatg cttctgccag cgattatcaa aacaatgaat ttcacggcag
    43741 gagtgaggca atggaaaaga aaatcggttt tattggctgc ggcaatatgg gaaaagccat
    43801 tctcggcggt ctgattgcca gcggtcaggt gcttccaggg caaatctggg tatacacccc
    43861 ctccccggat aaagtcgccg ccctgcatga ccagttcggc atcaacgccg cagaatcggc
    43921 gcaagaagtg gcgcaaatcg ccgacatcat ttttgctgcc gttaaacctg gcatcatgat
    43981 taaagtgctt agcgaaatca cctccagcct gaataaagac tctctggtcg tttctattgc
    44041 tgcaggtgtc acgctcgacc agcttgcccg cgcgctgggc catgaccgga aaattatccg
    44101 cgccatgccg aacactcccg cgctggttaa tgccgggatg acctccgtaa cgccaaacgc
    44161 gctggtaacc ccagaagata ccgctgatgt gctgaatatt ttccgctgct ttggcgaagc
    44221 ggaagtaatt gctgagccga tgatccaccc ggtggtcggt gtgagcggtt cttcgccagc
    44281 ctacgtattt atgtttatcg aagcgatggc cgacgccgcc gtgctgggcg ggatgccacg
    44341 cgcccaggcg tataaatttg ccgctcaggc ggtaatgggt tccgcaaaaa tggtgctgga
    44401 aacgggagaa catccggggg cactgaaaga tatggtctgc tcaccgggag gcaccaccat
    44461 tgaagcggta cgcgtactgg aagagaaagg cttccgtgcc gcagtgatcg aagcgatgac
    44521 gaagtgtatg gaaaaatcag aaaaactcag caaatcctga tgactttcgc cggacgtcag
    44581 gccgccactt cggtgcggtt acgtccggct ttctttgctt tgtaaagcgc caaatctgcc
    44641 gatttcaacc actcacgata gtgactcatt tgtgggttca gcggcgcaac ccccacacta
    44701 atccgtaaag ttacctgtgg cgtattcggc aagcgtaacg tgtttagccc ttcacgcacc
    44761 cgtaacatgg cggtaatggc gctctcagct ggcgtaccgg acatgattac tgcaaactca
    44821 tcgccgccaa accgaccaat cacatcgcta ccgcgcaggg taatttgtaa ctgtcgggta
    44881 agcgccacaa tcgcttcatc gcccacatca tgaccccagg tatcgttgat gctcttgaaa
    44941 tggtcgatat cgataatcag taacgttgca tcgcgattat gccgccgaca gttatcaaat
    45001 tcattgcgta acatagtttc ccaatgacgt cggttataca cgcccgtcat gccgtcgcga
    45061 gtactcatga cctgcaacct gcgtttatgt tccgccagtt tggttgccgt ctggtagctg
    45121 acccagccaa acatcagagg ataaacgaca ataatgggaa gggagagcca ccattccagc
    45181 ggagcactat tgaacgacac ggtaatgccc gtaagctcga gggtgacaag gcaggaaacc
    45241 accatcaaca ccagacccgc gacaaacaga cgggggccgc ctgcccccat caaattcaga
    45301 cacagaatca tcaacatagc ggtggaaggc agcacgttta cgcccattac gcctacccac
    45361 attcccgcta atactgcatc ggtttttaag ttgtaaattt cccggctaag cggatcgacg
    45421 gccctgctcg ctatctgcca ggctaaatgc ggccagacga acgcccagcc gaccaacacc
    45481 agccaccacc agcccggcgg cgggtgcgaa accagcgttg aagcaatcgg taagaacatg
    45541 ccagccaggc caaccgcacg gggtagtctg acgcgacggg cgaagcgcag cccggaccgc
    45601 tgatgttcgt tttgaggagt taaaggaggt tcctccccat gcgccgccgc ttttttgaaa
    45661 aagttttcat cattcattat ttttgggaac attcagaaac aattttccca aattatagag
    45721 acggggctaa aggcagaagt atgaaatttc gggtgagcca ctttcgtagc tcacccgtta
    45781 gcact
//
LOCUS       DAJTSI010000014        44957 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000014 DAJTSI010000000
VERSION     DAJTSI010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44957
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.014"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..509)
                     /gene="nudK"
                     /locus_tag="OO327_000708"
     CDS             complement(<1..509)
                     /gene="nudK"
                     /locus_tag="OO327_000708"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR6017363.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTG"
     gene            complement(577..2556)
                     /gene="aegA"
                     /locus_tag="OO327_000709"
     CDS             complement(577..2556)
                     /gene="aegA"
                     /locus_tag="OO327_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR6017364.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            2762..4479
                     /gene="narQ"
                     /locus_tag="OO327_000710"
                     /pseudo
     CDS             2762..4479
                     /gene="narQ"
                     /locus_tag="OO327_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            4681..7794
                     /gene="acrD"
                     /locus_tag="OO327_000711"
     CDS             4681..7794
                     /gene="acrD"
                     /locus_tag="OO327_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCR6017365.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(7893..7952)
                     /gene="ypfM"
                     /locus_tag="OO327_000712"
     CDS             complement(7893..7952)
                     /gene="ypfM"
                     /locus_tag="OO327_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR6017366.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            8333..8689
                     /locus_tag="OO327_000713"
     CDS             8333..8689
                     /locus_tag="OO327_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR6017367.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            8693..9820
                     /gene="dapE"
                     /locus_tag="OO327_000714"
     CDS             8693..9820
                     /gene="dapE"
                     /locus_tag="OO327_000714"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR6017368.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            9848..10048
                     /locus_tag="OO327_000715"
     CDS             9848..10048
                     /locus_tag="OO327_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR6017369.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(10158..10856)
                     /gene="ypfH"
                     /locus_tag="OO327_000716"
     CDS             complement(10158..10856)
                     /gene="ypfH"
                     /locus_tag="OO327_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6017370.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(10930..12945)
                     /gene="tmcA"
                     /locus_tag="OO327_000717"
     CDS             complement(10930..12945)
                     /gene="tmcA"
                     /locus_tag="OO327_000717"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR6017371.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(12960..13823)
                     /locus_tag="OO327_000718"
     CDS             complement(12960..13823)
                     /locus_tag="OO327_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR6017372.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(13991..14704)
                     /gene="purC"
                     /locus_tag="OO327_000719"
     CDS             complement(13991..14704)
                     /gene="purC"
                     /locus_tag="OO327_000719"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR6017373.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(14917..15951)
                     /gene="bamC"
                     /locus_tag="OO327_000720"
     CDS             complement(14917..15951)
                     /gene="bamC"
                     /locus_tag="OO327_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR6017374.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(15968..16846)
                     /gene="dapA"
                     /locus_tag="OO327_000721"
     CDS             complement(15968..16846)
                     /gene="dapA"
                     /locus_tag="OO327_000721"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR6017375.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            16992..17564
                     /locus_tag="OO327_000722"
     CDS             16992..17564
                     /locus_tag="OO327_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR6017376.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            17564..18034
                     /gene="bcp"
                     /locus_tag="OO327_000723"
     CDS             17564..18034
                     /gene="bcp"
                     /locus_tag="OO327_000723"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR6017377.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            18287..18904
                     /gene="hyfA"
                     /locus_tag="OO327_000724"
     CDS             18287..18904
                     /gene="hyfA"
                     /locus_tag="OO327_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR6017378.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            18904..20921
                     /gene="hyfB"
                     /locus_tag="OO327_000725"
                     /pseudo
     CDS             18904..20921
                     /gene="hyfB"
                     /locus_tag="OO327_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            20932..21879
                     /gene="hyfC"
                     /locus_tag="OO327_000726"
     CDS             20932..21879
                     /gene="hyfC"
                     /locus_tag="OO327_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR6017379.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            21896..23335
                     /gene="hyfD"
                     /locus_tag="OO327_000727"
     CDS             21896..23335
                     /gene="hyfD"
                     /locus_tag="OO327_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCR6017380.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            23347..23997
                     /gene="hyfE"
                     /locus_tag="OO327_000728"
     CDS             23347..23997
                     /gene="hyfE"
                     /locus_tag="OO327_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR6017381.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFATEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            24002..25588
                     /gene="hyfF"
                     /locus_tag="OO327_000729"
     CDS             24002..25588
                     /gene="hyfF"
                     /locus_tag="OO327_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR6017382.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            25578..27292
                     /gene="hyfG"
                     /locus_tag="OO327_000730"
                     /pseudo
     CDS             25578..27292
                     /gene="hyfG"
                     /locus_tag="OO327_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            27302..27847
                     /gene="hyfH"
                     /locus_tag="OO327_000731"
     CDS             27302..27847
                     /gene="hyfH"
                     /locus_tag="OO327_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR6017383.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            27844..28602
                     /gene="hyfI"
                     /locus_tag="OO327_000732"
     CDS             27844..28602
                     /gene="hyfI"
                     /locus_tag="OO327_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR6017384.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            28595..29008
                     /gene="hyfJ"
                     /locus_tag="OO327_000733"
     CDS             28595..29008
                     /gene="hyfJ"
                     /locus_tag="OO327_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR6017385.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            29038..30867
                     /gene="hyfR"
                     /locus_tag="OO327_000734"
     CDS             29038..30867
                     /gene="hyfR"
                     /locus_tag="OO327_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCR6017386.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMA"
     gene            30868..31050
                     /locus_tag="OO327_000735"
     CDS             30868..31050
                     /locus_tag="OO327_000735"
                     /inference="COORDINATES: protein motif:HMM:NF014950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HCR6017387.1"
                     /translation="MLHPTTPENDEEERQRIVQVLRETNGIVAGPRGAATRLGMKRTT
                     LLSRMQRLGISVREVL"
     gene            31072..31944
                     /locus_tag="OO327_000736"
                     /pseudo
     CDS             31072..31944
                     /locus_tag="OO327_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(31982..33043)
                     /locus_tag="OO327_000737"
     CDS             complement(31982..33043)
                     /locus_tag="OO327_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6017388.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            33256..34719
                     /gene="bepA"
                     /locus_tag="OO327_000738"
     CDS             33256..34719
                     /gene="bepA"
                     /locus_tag="OO327_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR6017389.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQACYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQKRFKPYTKM"
     gene            34740..35099
                     /gene="arsC"
                     /locus_tag="OO327_000739"
     CDS             34740..35099
                     /gene="arsC"
                     /locus_tag="OO327_000739"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR6017390.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(35232..35978)
                     /gene="hda"
                     /locus_tag="OO327_000740"
     CDS             complement(35232..35978)
                     /gene="hda"
                     /locus_tag="OO327_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR6017391.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(36028..37317)
                     /gene="uraA"
                     /locus_tag="OO327_000741"
     CDS             complement(36028..37317)
                     /gene="uraA"
                     /locus_tag="OO327_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR6017392.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(37403..38029)
                     /gene="upp"
                     /locus_tag="OO327_000742"
     CDS             complement(37403..38029)
                     /gene="upp"
                     /locus_tag="OO327_000742"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR6017393.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            38353..39390
                     /gene="purM"
                     /locus_tag="OO327_000743"
     CDS             38353..39390
                     /gene="purM"
                     /locus_tag="OO327_000743"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR6017394.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            39390..40028
                     /gene="purN"
                     /locus_tag="OO327_000744"
     CDS             39390..40028
                     /gene="purN"
                     /locus_tag="OO327_000744"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR6017395.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            40200..42266
                     /gene="ppk1"
                     /locus_tag="OO327_000745"
     CDS             40200..42266
                     /gene="ppk1"
                     /locus_tag="OO327_000745"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR6017396.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            42271..43812
                     /gene="ppx"
                     /locus_tag="OO327_000746"
     CDS             42271..43812
                     /gene="ppx"
                     /locus_tag="OO327_000746"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR6017397.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(43851..>44957)
                     /gene="pdeF"
                     /locus_tag="OO327_000747"
     CDS             complement(43851..>44957)
                     /gene="pdeF"
                     /locus_tag="OO327_000747"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR6017398.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        10406 a        11853 c        11697 g        11001 t
ORIGIN      
        1 ccggttttga tcatctccag cgcctggctg aacggcagct cgagcacttc aatatcttca
       61 tcttcaacac cgccaccggc gttagcgcgc tgattgtcac tgtattcggc gataaaaaag
      121 tggattagct cagtcacacc gccaggcgac atatacagtt caaataattt gcgcacttcg
      181 cccacttcat agcccgtctc ttcaatcgct tctttacgaa tgcacacttc cggttcgtcg
      241 ttatccagca gcccggcgca ggtttcaatc agctgcccgc tttcattgcc attaacccag
      301 gtagcgacac ggaactgacg aatcagaacc acgctctttt tcttcgcgtt gtacaggagg
      361 atcgtcgcac cattaccgcg atcgtaaact tcacgtttat ggcggataac ttcgccatct
      421 ttgcgcgtga gatcgtaagt aatgttgtgc agggtgaaat agttatcgga gagaattttg
      481 tctttaatga gggtgatttg ttgcgtcata ccgactccac agcgcgaaat gaacaattat
      541 attacgctgt gaagtcgggt ttgtctgcgc aggctatcag tgagatttga ctgattttac
      601 ccccagccag tcaataatcc cctgtgccgc atgacgtcct tctgccattg cggtaaccac
      661 cagatccgca ccacgcacgg cgtcgccacc ggcgaagatt ttcggattgg tggtctggta
      721 acggtactgg ctttccacat ccgcgatgat gcggccccat ttgtctaccg ttacaccgtg
      781 cgactccagc cacggcatcc cgtgtggatt gaagccaaac gccataatca ccgcatcggc
      841 tggcatgaca aattcactgc cctcaatcgg tacaggtcgc cgacgcccct gggcatccgg
      901 ctcgccaaga cgcgtgcgca ggaaacgaat tccgcaaacg tgaccttgct cattcagctc
      961 aagcgccacc ggctggacgt taaattcgaa gttggccccc tcttcgcggg cgttcttcac
     1021 ctctttcttc gagcctggca tgttagcttc atcacgacga taagcgcagg tgacgttact
     1081 cgcgccgtgg cgcagcgcag tacgcacaca gtccatcgcg gtgtcgccgc cgcccagtac
     1141 cacgacgtta agtccggcgg tattgataaa cggctcttcc ggtagctctt cgagccccat
     1201 cacctgttta gtgttggcaa tgaggaacgg cagcgcgtca taaacgcccg gcgcatcttc
     1261 attgggtaaa cccgctttca tggaacggta agtgcctacg ccaacgaaga ccgcgtcgta
     1321 ttgttccaaa agcaaatcca aagagacatc tttacccact tcacaattga gttcgaagtg
     1381 aatccccatc gcgctgaaga tttcccgacg gcgtgccagc agggatttat ccagtttgaa
     1441 agaaggaatg ccgaaagtga gcaagccacc gatttctgga tggcgatcgt acaccgtcac
     1501 cgccacgcca ttgcgggtca gaacatccgc acaggccagc cctgccggac ctgccccgat
     1561 aatcgccacc cgcttgtcca ctttggtgac atgacttaag tcaggacgcc aacctttcgc
     1621 caacgcctga tctgaaatat agcgttcaat gttgccgata gttaccgcgc cgtgctcatc
     1681 gcgaatagta caggcacctt cacacaaacg gtcttgcgga caaacgcgtc cggtaatttc
     1741 cggcagggtg ttggtctggt gagaaagctc gacggcggcg tcgatgtttc cggctttcac
     1801 cagttcaatc cactgcggaa tatggttatg cagcgggcag gtccattcac aaacgctgtg
     1861 ctcgccgaac ttaaggcagc gcgaggcttc ccgttgtgcc tggtcggcgc gaaatggcag
     1921 ataaatttca tcaaaaccgg ttttgcgcgc ttcaatcgcc agtttatccg gctcgccacg
     1981 cgcgggcgtt gcctgcattt gttcgacttt actcattacc ggcatttctt gtgcagcggt
     2041 actggcatgc cacggttgat gctcctgacg tgcggtgcgc aagcggcggg atttcgccat
     2101 gccggagagt gcgacgtcag tgaccagttg cagcgcgtcc gccgggcaat tctcgacaca
     2161 ggcaggaccg ttttcgcgcc ctgcacaaag gtcgcattta tgcgccgtgg cttttacttt
     2221 tcctgccgcg acgggtgtca ggacgatttg catcgtacca aacggacagg ccaccacgca
     2281 ggatttacag ccaatacact tttgctgatt gacctgaatg ctgtcatcaa cgtggctgat
     2341 tgcgccatta gggcagctac gggcgcaggg cgcatcttca caatggtgac aggtcactgc
     2401 actacgttgc tgctgatgtt tgataaccgt aattcgggga tgaaaatggt gttggctcag
     2461 gacatgttgc tcatcattgt gagccatgac acaggcgatt tcacaagcat gacaacccag
     2521 acattgctga ctgttggcca taataaaacg attcataacg accttctttt ttggttgtaa
     2581 aaaccttatt ctttatatga gtgttgttat tacccgactt acaggggaac cggcaatgtt
     2641 catgtgccca gaataagtaa ctatttcgct ataaactgtg gcagatcaaa taatcccatc
     2701 actgactaaa ttgcgtttca gcgaactgga acattaatga ttttttgtgg agaagacgcg
     2761 tgtgattgtt aaacgacccg tctcggccag tctggcccgg gccttttttt acattgtgct
     2821 gctgtcgatt ctttccacgg gtatcgctct gctaactctg gcgagcagtt tgcgcgacgc
     2881 tgaggctatc aatattgccg gatcgctgcg tatgcagagt taccgcctgg gctacgactt
     2941 gcaaagtggc agtccacaac tcaatgcaca tcgccagttg tttcagcagg cactgcattc
     3001 accggtatta accaacctca acgtctggta tgtgccagaa gcagtaaaaa ctcgctatgc
     3061 gcatctgaat gccaactggc tggagatgaa taatcggctc agcaagggcg atttgccgtg
     3121 gtatcaggcc aatattaata attatgttaa tcagatagac ctgttcgtgc tggctttaca
     3181 gcactacgct gaacgcaaaa tgctgctggt ggtggcgatt tccctggctg gcggtatcgg
     3241 tattttcacg ctggtctttt ttactttgcg ccgcatacgc catcaggtgg ttgccccgct
     3301 gaatcagctg gttaccgcca gtcagcgtat tgaacacggg cagttcgact cgccgccgct
     3361 ggataccagt ctgccgaatg agcttggtct gcttgcaaaa acctttaacc aaatgtcgag
     3421 cgagctgcat aaattgtacc tttcgctgga ggcgtcagta gaagaaaaga cccgcgatct
     3481 ccacgagacc aagcgtcgtc tggaggtgtt gtatcagtgc agtgttccca ggcgctgaac
     3541 accagccaga ttgatgtgca ttgtttccgc catattttgc agattgttcg cgacaatgaa
     3601 gcagctgaat atctggagct gaacgttggg gataactggc ggattagcga agggaagcca
     3661 aaccctgagc tgccaatgca gattttaccg gtgacaatgc aagagacggt ttacggcgaa
     3721 ctgcactggc aaaatagtca cgtttcatca tcagaaccgc tgcttaacag tgtttcatcg
     3781 atgctgggac gcggtttgta ctttaatcag gcgcagaagc attttcagca gttattgttg
     3841 atggaagaac gtgcgaccat cgcccgcgaa ttgcacgact cgctggctca ggtgctttct
     3901 tacttacgta tccagttgac gttactgaag cgttcgatac cggaagataa cgccaccgca
     3961 caaagtatca tggccgattt ttcccaggcg ttgaatgatg cttatcggca gttacgcgag
     4021 ctgttgacta ctttccgcct gacgctgcag caggcggatc tcccctccgc gttgagggaa
     4081 atgctggata cgttacaaaa tcaaaccagc gctaaactga ccctcgactg ccgtctgcca
     4141 accctggcac tggatgcgca aatgcaggtg catttgttgc aaattattcg cgaagcggtg
     4201 ctgaatgcga tgaagcacgc caacgccagc gaaatcgccg tcagttgcgt caccgcgccg
     4261 gacggcaatc acacggttta tatccgtgat aacgggattg gtatcggtga accgaaagaa
     4321 cccgaaggtc attatggact gaatatcatg cgcgaacgcg cggaacggct gggcgggacg
     4381 ctgacttttt cacaaccttc cggcggcggc acgttagtga gtattagctt tcgctccgca
     4441 gagggtgagg ggaaggggaa cacgccggct gaggtgtaaa taattaccct cacaccggcc
     4501 ctctcccttt aaaggcgaga gggaaaaacg tgcccatatt gacctgtaag cgagtgtgtc
     4561 agcaatctca tgataattta cattaactcc tttttttctc cacgattggc tcgtaccttg
     4621 ccgctacagt gaagcaagtc aagcctacaa caatacgcag aaacacgagg tcctctttta
     4681 atggcgaatt tctttattga tcgccccatt tttgcctggg tgctgacaat cctgttgtgt
     4741 ctgacaggta ccctggcgat tttttcattg cccgttgaac aataccccga tctcgcgcca
     4801 ccgaatgtgc gagtgaccgc taactatccc ggcgcatcgg cccagacgct ggaaaacacc
     4861 gtgacccagg ttatcgagca aaatatgacc ggcctcgata atctgatgta tatgtcatct
     4921 cagagcagtg gcaccggtca ggcatctgtc actttaagtt ttaaagcagg caccgatccg
     4981 gacgaagccg tgcagcaagt acaaaaccag ctgcaatcag ccatgcgaaa gttaccgcag
     5041 gcggtgcaaa atcagggcgt gacggtgcgt aaaaccggcg ataccaacat tctgaccatt
     5101 gccttcgtct ctaccgatgg ctcgatggat aaacaggata ttgctgatta tgttgccagt
     5161 aatattcagg acccgttaag ccgcgtgaat ggcgtcgggg atatcgatgc ctatggttcg
     5221 caatattcca tgcgtatctg gctggacccg gcgaaactca acagtttcca gatgacggct
     5281 aaagatgtca ctgatgccat tgagtcacag aacgcgcaga ttgcggttgg gcaacttggt
     5341 ggtacacctt ccgtcgataa gcaggcgctc aacgccacca ttaacgccca gtcactgctg
     5401 caaacaccag aacagttccg cgatatcacc ttgcgggtga atcaggacgg ctcagaggta
     5461 aggctgggcg atgtcgccac cgtcgaaatg ggggcggaga aatacgatta tcttagccgc
     5521 ttcaatggta agccagcctc cgggttgggg gtaaaactgg cctccagcgc aaacgaaatg
     5581 gcgacagcgg agctggtgct caatcgtctc gacgagctgg cgcagtattt cccgcacgga
     5641 ctggaataca aggtggcgta tgaaaccacc tcgtttgtta aagcctccat tgaagacgtg
     5701 gtgaaaacgc tgctggaagc tatcgctctg gttttcctcg ttatgtatct gttcctgcaa
     5761 aacttccgcg ccacgctgat acccactatc gccgtgccgg tggtgttgat gggaaccttc
     5821 tccgtacttt acgccttcgg ttacagcgtc aacaccttaa ccatgttcgc gatggtgctg
     5881 gcgatcggtc tgctggtgga tgacgccatc gtggtggtgg aaaacgtcga acgtattatg
     5941 agtgaggaag gactcactcc tcgcgaagcc acacgtaaat cgatggggca gatccagggg
     6001 gcactggtcg ggattgcgat ggtgctttcg gcggtatttg taccaatggc cttcttcggc
     6061 ggcaccaccg gtgccatcta tcgccagttc tctattacca ttgttgcggc gatggtgctg
     6121 tcagtactgg tagcgatgct cctcactccg gctctgtgtg ccacgctact taagccactg
     6181 aaaaaaggtg agcatcacgg gcaaaaaggt ttttttgcct ggtttaacca gatgtttaac
     6241 cgcaacgccg aacgctacga aaaaggggtg gcgaaaattc tccaccgtag cctgcgctgg
     6301 attgtgattt atgtcctgct gcttggcggc atggtgttcc tgttcctgcg tttgccgacg
     6361 tcgttcttac cgctggaaga ccggggcatg tttactacct cggtacaatt gcccagcggt
     6421 tcaacccaac aacagaccct gaaagtcgtt gagcaaatcg agaaatatta cttcacccat
     6481 gaaaaagaca acatcatgtc ggtgtttgcc accgttggtt ctggccctgg gggtaacggg
     6541 caaaacgtgg cgagaatgtt tatccgcctg aaagactgga gcgaacgcga cagtaagacc
     6601 ggcacctcgt ttgccattat cgagcgtgca acgaaggcgt ttaaccaaat taaagaagct
     6661 cgcgttatcg ccagcagccc accagcaatt agcggtcttg gtagttctgc aggttttgat
     6721 atggagttgc aggaccacgc tggagcgggt cacgatgcgc tgatggcagc acgtaatcag
     6781 ttgctggcgc tggcggcgga aaacccggag ctaacccgtg tgcgccataa cggcctcgat
     6841 gacagtccgc agttgcagat tgatatcgac cagcgtaaag ctcaggcgct gggcgttgat
     6901 atcgacgata ttaacgacac actgcaaacc gcctggggtt cgagctatgt gaatgacttt
     6961 atggatcgcg gtcgcgtgaa gaaagtctat gtgcaggcag ctgcgccgta tcgcatgctg
     7021 ccagatgaca tcaatctctg gtatgtccga aataaagatg gcggcatggt gcccttctct
     7081 gctttcgcga cctcacgctg ggaaacaggc tcgccgcgtc tggaacgcta taacggttat
     7141 tctgcggttg agattgttgg ggaagccgca ccgggggtca gtaccggtac ggcgatggat
     7201 attatggaat cgttagtgaa gcagctgcca aacggctttg gtctggagtg gacggcgatg
     7261 tcgtatcagg agcggctttc cggcgcgcag gctccggcgc tgtacgccat ttccttgctg
     7321 gtggtattcc tgtgtctggc ggcattgtat gaaagctggt cggtaccgtt ctcggtaatg
     7381 ctggtcgtgc cgctgggggt aatcggcgcg ctgctggcaa cctggatgcg cgggctggaa
     7441 aacgacgttt acttccaggt gggcctgtta acggtcattg gtttatcggc gaaaaacgcc
     7501 atcctgatcg tcgagtttgc taacgagatg aaccaaaaag gccatgacct gtttgaagcg
     7561 acgctccacg cctgccgtca gcgtttacgc ccgattctga tgacctcgct ggcatttatc
     7621 ttcggcgtat tgccaatggc aaccagcacg ggtgccggtt ccggtggtca gcatgcggtg
     7681 ggtactggcg taatgggcgg gatgatttca gctactattc tggctattta cttcgtgccg
     7741 ctgttctttg tgctggtgcg ccgccgcttc ccgctgaagc cgcgcccgga ataagcaata
     7801 aaaaaggcga catgccaatg tgtcgccttt ttcaactttc cgattaagaa cctgctcagc
     7861 gggttcttgc tgtttatact ttgtctcagg aattacttac gaagcataac ttcgataaag
     7921 tctttccagt tccccagttc acgttcaatc ataacaacct ctcttataat tatgggtatt
     7981 ctacggaaac aatataccgt ggtgaagcta atttactcga ttgctgcgat gactacctcc
     8041 gggggacaaa ccttatgtaa atactatggt cctgcggtga taatttgtat gtgatacaca
     8101 gcaacatttc gagatattca tacggcatct aatactgatt taattctggt taaaatacag
     8161 acagataaca agatgaatat tcttaatgtt tacgttaaaa atgtttaata ttatttaata
     8221 gttgttaatt tgaatacttc gataatgtta tattttctga taatcatttg caggcaaaat
     8281 gttttcaccc ttaaatgagt atttattctc ataaatcgaa aaaggattca ttatggttac
     8341 actttacggc atcaaaaatt gtgacaccat taaaaaggct cgccgttggc tggaagccaa
     8401 taacatcgac tatcgttttc atgattaccg cgtcgatggg ctggacagcg aattattgaa
     8461 cggttttatc aacgaattag gctgggaagc gttactcaac acccgtggta caacctggcg
     8521 taaactggac gaaactaccc gcaataaaat caccgatgcg gcctctgcgg cggcattaat
     8581 gactgaaatg cctgcaatta tcaaacgtcc attgctctgc gcgcctggta agcctatgct
     8641 gctgggtttc agtgattcca gttatcagca atttttccat gaggtgtagt ctatgtcgtg
     8701 cccggttatt gagctgacac aacagcttat tcgccgccct tccctgagtc ctgatgacgc
     8761 aggatgtcag gctttgttga ttgaacgttt gcaggcgatc ggttttaccg ttgaacgcat
     8821 ggattttgcc gatacgcaga atttttgggc atggcgtggg caaggtgaaa cgttagcctt
     8881 tgccgggcat accgacgtgg tgccgcctgg cgacgccgat cgttggatca atccgccgtt
     8941 tgaacccacc attcgtgacg gcatgttatt cgggcgcggt gcggcagata tgaaaggctc
     9001 gctggcggcg atggtggtag cagcagaacg ttttgtcgca caacatccca accatacggg
     9061 gcgactggca tttctgatca cttctgatga agaagccagt gcccacaacg gtacggtaaa
     9121 agtcgtcgag gcgttaatgg cacgtaatga gcgtctcgat tactgcctgg tcggcgaacc
     9181 gtcgagtatc gaagtggtag gtgatgtggt gaaaaatggt cgtcgcggat cgttaacctg
     9241 caacctcacc attcatggcg ttcaggggca tgttgcctac ccacatctgg ctgacaatcc
     9301 ggtacatcgc gcagcacctt tccttaatga attagtggct attgagtggg atcagggcaa
     9361 tgaattcttc ccggcgacca gtatgcagat tgccaatatt caggcgggaa cgggcagtaa
     9421 caatgttatt ccgggtgaac tgtttgtgca gtttaacttc cgcttcagca ccgaactgac
     9481 tgatgagatg atcaaagcgc aggtacttgc cctgcttgaa aaacatcaac tgcgctatac
     9541 ggtggattgg tggctttccg ggcagccatt tttgaccgcg cgcggtaaac tggtggatgc
     9601 ggtcgttaac gcggttgagc actataatga aattaaaccg cagctactga ccacaggcgg
     9661 aacgtccgac gggcgcttta ttgctcgcat gggggcgcag gtggtggaac tcgggccggt
     9721 caatgccact attcataaaa ttaatgaatg tgtgaacgct gccgacctgc agctacttgc
     9781 ccgtatgtat caacgtatca tggaacagct cgtcgcgtga tgagtggttc tgcaagagga
     9841 aataagcatg gactggctgg ctaaatattg gtggattctg gtgattgtct ttttggtagg
     9901 cgtcctgctg aacgtgatta aagatctcaa gcgcgtcgac cataagaaat ttctcgccaa
     9961 caagccggag cttcccccgc atcgtgattt caacgataag tgggacgatg acgacgactg
    10021 gccgaaaaag gatcaaccga agaagtaagc tgagtgctca accgtaggcc ggataaggcg
    10081 ttaacgccgc atccggcaat ggtgaacgat ccctgatgcg acgctcacgc gtcttatcag
    10141 gccaggtaat ctgtctttta catcatttca atcacatcat catcgccagg cttaccgcca
    10201 cttagcgctt ggtcgaaata atgtttcgga atggtataac gcaagtgatc cagggcaaat
    10261 tgcatactgc gattgtcgat cgcatggcct aaatcctcca cgatatccag cgtcacgtca
    10321 ccaccggcac tgattaacgc ttcctgcgcc gcaactgcat gcgccagatc gataaccgga
    10381 tcttcaccac catgaatcaa gtgaattgtc gtggctgtag acgcggtctc cggcaggctc
    10441 gcatagcgtc cgttaaaggc aatgacgcgc gaagcaaggc ccggttcggc tttaatgctc
    10501 tccagcgcca taatcgcgcc ttgtgagaaa ccaattagcg ccgtggcgtt agcccctact
    10561 ccgctctgtt tctgccagta gcgtaccgtt tcaataaacg tcggcatgat ggcatccaca
    10621 cgcgcctggc gattatcttc cgtaataccc tgcactgaaa accactgacg ccccgccgga
    10681 ttaccgctcg gctccgcgcc accgacactc accaccagcg catcaggaaa cagcggtgca
    10741 aaccagctgc ctatttcccc cattgccacc gggttatccc cgacaccgtg gaaaagcagt
    10801 aacaactgtt gtgcaggttt atccgggctt tgaacaacaa aatggtcatg tttcatggcg
    10861 aactccttaa ctgatgtcat taattttacg ccgctgactc acaatgacca tgggagaaaa
    10921 ttgaaggagt cagtgaaaaa attgccattg cgttatgcga tcgcgcagac gctcagtgcg
    10981 aacctcattt agtgcgaaca gcgcctgcgc ggcctcttcc cgctggcgaa ccagtaacat
    11041 cttgcggcct gaaagtttaa gtgtggtaca taactgcgcg tcactggcgt ttttctgtaa
    11101 acgcccacgc agcgccggta atgccagttc actggtttgt agcagacgca ataagcaacc
    11161 taacgatgtt aatagcggac gatgagcgaa agcaaaaccg gccagttcaa gccagtcgtc
    11221 gtcagaaagg atggcatcgt ttagtggatc aacaggaagc atttcgccat tccactgcgc
    11281 gagagcttgc gcatcgcgac gtaaacggta atgctcacgt tcagccagct gtttacccgc
    11341 atcactcatc ggtaacagcg ccatcgccgt atagcaaccg ctgctggctt cccgatgatt
    11401 acccatccgc accagcacaa aaccgcagcg ttgccagaaa cgccataact ccccagtgta
    11461 accaaaactc accgaaagat agtcgaggtc atgcgtatat tgcaaagctc cagcaataag
    11521 ttgtcgccct gtgccttccc gctgacgagc cggatgaact gctatccggc taacccgcct
    11581 tccgcgcaat gtcgccgcca gtggattgct gccgtgcgcc gccagcgact gggccaccag
    11641 attaccccgc gggcgacgaa aacctgccca taccgcctga ctgagttctt gagataatcc
    11701 gccctcatcc accagccaca acgccccggc aatctcgttt tcgccagccg cctgtaaaaa
    11761 atgttgccct ggtgcatcca tcatccggcg taaatccagc ggcgaggtcc ggtagtgcgc
    11821 accagacaat aactgataaa cctttaacgg cgtttctggc tcgcttcgcc ataacgtttg
    11881 ttcaaatgcg gaaatgacga tattgccttg tggtgtatgg gtgaagtttt catcgttaaa
    11941 aaccagtgcc tcactaacca ttttttccag cgggcatccc tgtgcccagc ggatcggctg
    12001 ttgcagttca aaacggtgta aatgcggaaa gcgagcgcaa aatttcagca aaaaaccacg
    12061 tccggtacct tcgtagccct gcaccgtagt ggttaacaac gttcgaggaa aacgcgatac
    12121 cagttgatgc aacaacggcg caggtatggc tgcggcttcg tcgaccacca gccagtcggc
    12181 ttgctcatcg ctggctaaca aggcatccgg tgcaataaag cgaaacttct cgcccgcaaa
    12241 ttgtgccagt acatccgttg ccgcttttgc gggcgcggtg acaatcgcac tgcccgcaat
    12301 acgagaaatg agttgccctg ccaacgccga cttaccgcgc ccacgcgcag ccgttaccgc
    12361 tgccacgccc ggcggcatgg tcagtagctg ctgtaagagt tgctgttgtt ctggttgtgg
    12421 tgcgccggtc gcggggtgcc agtcagtacg gggagtaaaa tgcgccaacg agaacggctg
    12481 gttttgccgc cagaggatag cgtcgttatc cgccgtaagt acgcgtttga gatgctggac
    12541 aaaatgcggc gtcgcaatag ggtcagggca atcactccag cgcagcgagt cggcatcagg
    12601 ttggttttcc cactcttccc atacagggag taacaaaacc agccagcttc ccgctttcaa
    12661 cgttccgcta agtgctgcaa aggcagcggc atcaaagccg tggcgggcgt cgaataccgc
    12721 atgccggaac tcgcgcccaa gtaaagtttg tagcgccgag ggtgaacagt ggttttcagc
    12781 atctagctgc ggcgaaatcc acagccagtc gccaggtaag gcatcacgca acttaagagc
    12841 atgatcaaaa caccaaccct cttccccgct caacaccagc aagcggcaga tcccttcacg
    12901 tttcatttgc gctgttaatg tgtaaagcgc agtcagttca gccatccctg ccccggaaat
    12961 taaatgcttt taccaaaagt attgcattgt gccggatcgc cgctgtcgaa accacgttta
    13021 aaccagctgt agcgttgctg agaagtgcca tgtgtgaaac tgtctggtac tactcgcccc
    13081 tgactttgct gttgtaaacg gtcatcgccg atggcctgcg ccgcattcag cgcctcttcc
    13141 agatcgccag tttccagaac gccttgctgc tgcatactat gcccccagac accggcaaaa
    13201 cagtcggcct ggagttccat acgcacagat aagcggttta cttccgcctg cgtcgcgttt
    13261 tgttgcagtt gacgaacttt cggctcgatg cctaacagtt tctgcacatg atgaccgact
    13321 tcatgggcga taacgtaccc ctgggcaaaa tcgccatccg cgccaagttt gtctttcatg
    13381 tcatcataga aggagagatc gatataaact gtgccatccg ccgggcaata gaacggcccc
    13441 ataatggact ggcccgcccc gcagccggtg cgcgtcattc cacggtacat gaccagcttc
    13501 ggttgctgat aggtcttacc catcttttcg aactgttgtc cccaggtgtc ttccgtggtt
    13561 gcctgaatca ccgaggtgaa ttttgcggct tcgtcttcat tagggctaat tgaccgcgtt
    13621 gattgttgtt gagaaaccgg ctgcccggtc atcaacccgg ttaaatcaac gccatagtag
    13681 cctgcaacca gcacgactat cagtaaaatc agcccgcctt taccgctcgg cagacggaaa
    13741 ccgggaccgc ccattgacgg accgccagag ctgttgcgcc tgtcttcaac attgtcactt
    13801 tcacgtcgcc cttgccaacg catagatacc tcaacaatat attcattata gaaatgatcg
    13861 taggtggttc agaggaagat taccacagga aagcgggatg aaatggcgga ggaatgagcg
    13921 ttcaatgcat atattccaag agcggttgtc ttgccgtgcc agctgcacgg caagatgatg
    13981 aacagaaaaa tcagtccagc tgtacaccca ggcggcgggc gacggcttca taggcttcga
    14041 tcaggccacc gaggctctgg cggaaacggt ctttgtccat tttctccagt gtttctttgt
    14101 cccacaggcg gctgccgtcc ggggagaact catcgcccag taccacttca cctttgtaca
    14161 gaccaaactc cagcttgaag tcgaccagaa tcagaccagc atcatcgaac agttttttca
    14221 gcacgtcgtt cgctttgtag gtcagctctt tcatacgcgc caggttctct ttgctcaccc
    14281 agccaaaggt ttcgcagtaa gattcgttga ccatcggatc gtgcatggca tcgtttttca
    14341 ggaacagatc gaacagcggc gggttcagct caataccttc ttcgattcca agacgtttca
    14401 ctagagagcc agcagcacgg ttacgcacga cacactcaac cggcaccata tccagctttt
    14461 tcaccagaca ttcggtatcg gagagcagac gctccatttg agtcgggata cccgcttcag
    14521 ccagtttgct cataatgaag tagttgaact tgttgttcac catacctttg cgatcaaact
    14581 gctcaatgcg cgcgccatcc cctgctgacg tatcattgcg gaattcgagc accaacaggt
    14641 ccgggttttc cgtgctgtat acggttttcg ctttaccacg atacaactca gcttgctttt
    14701 gcatctttat cactcctggg tgtgaattaa cgttttaaaa tcttttgctg tctggtgtgc
    14761 cggatgtttt gtcggatgcg gcgtgaacgc cttatccgaa ctacgtccga caaacaaatt
    14821 tcgtgcgaat taccgctaaa atcgcgtttt cctgccgacg cacacgtttg cgtatcatat
    14881 cagaaaaaag ggccggatga ttccagccct gtatttttac ttgctaaacg cagcctggaa
    14941 gacagctacc agcgcgtcgt tctgactctg agtcagagta tgacctttcg gatcgatgaa
    15001 ctgtaggctg ctgcggttat ctaaatcgcc aacctgcagt ttatagtcac cggatgccag
    15061 gcctggatcg ctcgcgccca gttcctgcca gtcgctgtca gacagcggct tataagttac
    15121 ggccatgttg ccctgcgaac gggtgctgtc ggtcactttc atgcccactt tttccagcgc
    15181 cgctggcaga cgttgccaaa ccacattgaa cggcccgcgt acgaccagca ttggtaaacc
    15241 ggtgtcatca gctgcacttt gtacgtccat agtggtggag gcacgatttt gcgcagcgtt
    15301 cgcggcgtca gtggcagatt tatccagacc ggcggaaata acgttcatca tctccgtgct
    15361 gtaacgctgc atggaagccg cgtctgcaac cggtttgccc gcctgttcca ggttcagcag
    15421 tttaaccgta accgcctgct gataaccctg cggcttaaca gagatttgat aacgaccacg
    15481 atactgctcg tcttcgtcca gacggttcca ttgtacccaa tcggtggtca gtgtctgacc
    15541 agcatcatca cgttgggtga tggtgtagtt tttcgcctgc agcacgctaa ccacctgcgg
    15601 ccacagagta ttgccacgac cattttccac cagcaatgaa gcggtatcgc ccgtgaactg
    15661 ggtacgcgcg ccagaaacca gtgccaacgg ctgggctggt ggacgaatgt ccagcgcctt
    15721 accgacagca ccactaccgt tggtcaccgg gattgcataa tcaccggagg tcaccggcaa
    15781 aatcattcca gccggggcat gaagctccgc aagcggtgcc gcttccaggt aggcttcatc
    15841 accactgacc tgacgcttat agcgtgagtc agaactacag gcagcgagta ataaaacaag
    15901 cgaaacaccc gcaacctttg ccaggcgcga cttttgaaca gagtaagcca tcaaatctcc
    15961 ctaaacttta cagcaaaccg gcatgcttaa gcgccgctct gaccgtctca cgaccactgt
    16021 cggtgattgg tgtcattggc aggcgcagcg tatcggtcgc cacaagaccc agttccttac
    16081 atgcccattt caccgggatt ggattgggtt cgacaaatag tttgttgtgt aatggcatca
    16141 gacgctgatt aataacgcgt gcctcggcaa aatgcccttc tgctgccagt ttgcacatct
    16201 gggccatatc acgcgctgcg acgttagccg taacggaaat aactccatga ccgcccaatt
    16261 gcatgaagtc cagcgcgctc gcatcatcgc cgctcagcag aacaaaatca tctgaaacca
    16321 gctctttgat ctggtttaca cgcgttaagt tccctgttgc ctctttgatt ccgataatat
    16381 tttttacttt cgccagacgg cccaccgttt ccgggagcag atcgcagcca gtacgggacg
    16441 gcacattata cagaatttgc ggcaggtcag tatgctcagc gatggctttg aaatgctgat
    16501 acaaaccttc ttgcgacgga cgattgtagt aaggggttac cgtcaggcag ccgacgatac
    16561 cactgtcatt gaagcgctgc gtcaggctaa tggcttccgc agtagcgtta gcgccggtcc
    16621 cggcaattac cggaatgcgc ccatcagcca gatccagcgt catcatcacc acatcagcat
    16681 gttcgtcatg atttaaggta gcggactcgc cagtggtgcc aacagaaacg atcgccgaag
    16741 taccgctggc gacatgataa tcaatcagtt ttttcaagct agcccgacag acattacctt
    16801 tttcatccat cggagtaaca atcgcgacaa tacttcccgt gaacatgggc catcctctgt
    16861 gcaaacaagt gtctcaatgg tacgtttggt atggcattaa aagcaagcag acagaaccgt
    16921 tctgattgtt gtatgcatga tttttttatg ctttccttaa gaacaactca ccccttaaag
    16981 gaataaccag tttgacactg tcatcgcaac attatctggt gatcactgcg ttgggtgccg
    17041 atcgccctgg aattgtgaac accatcaccc gtcatgtcag tagttgcggc tgtaatattg
    17101 aagacagtcg cctggcgatg ctgggagaag agttcacgtt tatcatgctg ctttccggtt
    17161 catggaatgc catcactctg attgaatcaa cgttaccgtt gaaaggtgcc gaactggatc
    17221 ttttaatcgt gatgaagcgc acgacggcgc gtccgcgtcc gccaatgcca gcatctgtct
    17281 gggttcaggt cgatgttgca gactccccgc atttaattga acgcttcaca gcacttttcg
    17341 acgcgcatca tatgaacatt gcggagctgg tgtcgcgcac acaacctgct gaaaatgaac
    17401 gggctgcgca gttgcatatt cagataaccg cccacagccc cgcatctgcg gacgcagcaa
    17461 atattgagca agcgttcaaa gccctatgta cagaactcaa tgcacaaggc agtattaacg
    17521 tcgtcaatta ttcccaacat gatgaacagg atggagttaa gtaatgaatc cactgaaagc
    17581 cggtgatatc gcaccgaaat ttagcttgcc ggatcaagac ggagaacaag ttaatttgac
    17641 cgacttccag ggacagcgtg ttctggttta tttctacccg aaagccatga cccccggctg
    17701 taccgtacag gcctgcggct tacgcgataa catggatgag ttgaaaaaag cgggcgttga
    17761 tgtgctgggt atcagcaccg ataaacccga aaaactctcc cgttttgcgg aaaaagagct
    17821 gcttaacttt acgctcctgt ctgatgagga ccaccaggtg tgcgaacaat tcggcgtctg
    17881 gggtgaaaag tccttcatgg gcaaaaccta cgatggtatt catcgcatca gcttcctgat
    17941 tgacgctgat ggcaaaatcg aacatgtctt tgacgatttc aaaaccagca atcaccacga
    18001 cgttgtgctg aactggctga aagaacacgc ctgattactt tgctccattc cgtgctggct
    18061 gcgcttgcgg ccagcatacc tcacttctcg tgatcaagat cacattctcg ctttcccctg
    18121 cgacacgggt gtcgaatcca ttttttgctg aacgttaatg accatcattt ttgtaccgtt
    18181 cagaatccag ttaatacata acttattgaa tagattgagt taatcagaat ggcatccttt
    18241 atacaatatg aaatgcaatg tttcatatca ttttcaagga gccgacatga accgctttgt
    18301 ggtggccgaa ccactgtggt gtacaggatg taatacctgt ctcgctgcct gttcggacgt
    18361 gcataaaacg caaggtttac agcaacaccc gcgcctggcc ctggcgaaga cgtcaacaat
    18421 cactgcccct gtcgtgtgtc atcactgtga ggaagcccct tgcctgcagg tctgcccggt
    18481 caatgccatc tctcagaggg atgatgcgat ccaactcaac gaaagcctct gtattggctg
    18541 caagctttgc gccgtggtct gcccatttgg cgcaatcagc gcttcaggaa gccgtccggt
    18601 gaatgcccat gcgcaatatg tttttcaggc tgaaggctca ctcaaagacg gcgaagaaaa
    18661 cgtgccgccc caacatgcgt tgctgcgctg ggaaccgggt gtccagaccg tcgcggtgaa
    18721 atgcgacctg tgtgatttct tgccagaagg tccggcctgc gttcgcgctt gcccgaatca
    18781 ggcgttacgg ctgatcaccg acgatagcct gcaacgtcag atgaaagaaa aacagcgcct
    18841 tgccgcaagc tggtttgcca atggcgggga gtctcccctt tccctcactc aggagcaaca
    18901 ctaatggatg ccctgcaatt attaacctgg tcgctgatcc tctatctgtt tgccagtctg
    18961 gcttcgctgt ttttactcgg tctggacaga ctggctatta agctttccgg catcacatcg
    19021 ctggtgggcg gcgtgattgg catcatcagc ggaattacgc aattacatgc tggtgtaact
    19081 ttagtcgccc attttgccac cccttttgac tttgccgatt taaccctgcg aatggatagc
    19141 ctctcggcat ttatggtgct agttatctcc ttgctggtgg tggtttgttc gctctattca
    19201 ttgacttata tgcgcgaata cgagggcaaa ggcgcggcgg cgatgggctt ctttatgaat
    19261 cttttcatcg catcgatggt tgccctgctg gtgatggaca acgctttttg gttcatcgtg
    19321 ctgtttgaaa tgatgtcgct gtcttcctgg tttctggtca ttgccaggca ggataaaacg
    19381 tcgatcaacg ctggcatgct ctactttttt atcgcccacg ccggatcggt gctgattatg
    19441 atcgccttct tgctgatggg gcgcgaaagc ggcagcctcg attttgccag tttcgcacgc
    19501 tttcactttc tccggggctg gcgtcggcgg tgtttctgct ggcctttttc ggttttggcg
    19561 cgaaagccgg gatgatgccg ttgcacagct ggttgccgcg cgctcaccct gccgcaccat
    19621 cgcacgcttc ggcgttgatg tctggcgtaa tggtcaaaat cggtattttc ggcatcctga
    19681 aagtagcgat ggatctgctg gcgcaaacgg gtttgcctct gtggtggggc attctggtga
    19741 tggcgatcgg cgcaatctcc gcgctcctgg gcgtgctata tgcgctggcg gaacaggata
    19801 tcaaacggct gctggcctgg agtaccgtcg aaaacgtcgg cattattttg ctggcagtcg
    19861 gtgtggcgat ggacggtctg tcactgcacg acccgctgct caccgtggtt ggactgctcg
    19921 gcgcactgtt tcatctgctc aaccatgcgc tgttcaaagg gctgctattt ctcggcgcgg
    19981 acgcgattat ttcgcgtttg catacccacg acatggaaaa aatgggggca ctggcgaaac
    20041 ggatgccgtg gacagccgca gcatgcctga ttggttgcct cgcgatatca gccattcctc
    20101 cgctgaatgg ttttatcagc gaatggtaca cctggcagtc gctgttctca ctaagtcgtg
    20161 tggaagccgt agcgctacaa cttgcgggtc ctattgctat ggtaatgctg gcagtcactg
    20221 gtgggctggc agtaatgtgc ttcgtcaaaa tgtacggtat tactttctgt ggtgcgccgc
    20281 gcagtacaca cgctgaagag gcacaggaag tgccaaatac gatgatcgtc gccatgctac
    20341 tgctcgcggc actctgcgta ttaattgcgc ttagtgccag ttggctggca ccgaagataa
    20401 tgcatattgc ccatgcgttt accaataccc ctcccgtcac tgtcgccagc ggaatagcac
    20461 ttgtacccgg cacgtttcat acacgggtca ctccttcatt actgttgctg ttactactgg
    20521 cgatgccttt gctgccaggc ctttactggc tgtggtgtcg ttcgcgccgc gcagcgtttc
    20581 gtcgcacagg agatgcctgg gcatgcggct acggctggga aaacgcgatg gctccgtcag
    20641 gcaatggcgt gatgcagccg ctgcgtgtgg tcttctctgc gttatttcgt ctacgacaac
    20701 agctcgaccc tacgctgagg ctaaataaag gtcttgcgca cgtcaccgcc agggctcaga
    20761 gcacagaacc cttctgggat gagcgggtga tccgccccat cgtgagcgcc acccaacggc
    20821 tggtcaaaga aatacagcat ctgcaaagcg gcgactttcg tctctattgc ctgtatgtgg
    20881 tcgccgcact ggttgtgctg ctaatcgcta ttgccgtcta aggaaatcac catgagacaa
    20941 actctttgcg acggatatct ggtcattttt gcgttagcac aggccgtgat tctgctgatg
    21001 ctaaccccac tttttacggg tatttcccgg cagatacgcg cgcgtatgca ctcccgccgc
    21061 gggccgggga tctggcagga ttatcgcgat atccacaaac tgtttaaacg ccaggaagtt
    21121 gcgccgacat cttcaggtct gatgttccgc ctgatgccgt gggtattaat cagcagcatg
    21181 ctggtgctgg cgatggcctt accactattt attaccgttt ccccttttgc gggcggcggc
    21241 gatctgatca cccttatcta tcttcttgcc ctgtttcgtt ttttctttgc tctttccggg
    21301 ctggataccg gaagtccgtt tgcgggagtc ggtgccagtc gcgagttgac gctcggcatt
    21361 ctggtcgaac caatgcttat tctctcactg ctggtattgg cgctgatagc aggttccacg
    21421 catatcgaga tgatcagcaa tacgctggct atgggctgga actcgccgct aaccaccgta
    21481 ctggcgttac tggcctgtgg ttttgcctgc ttcattgaga tgggaaaaat tccctttgat
    21541 gttgctgaag cagaacagga attacaggaa ggcccgctga ccgaatattc cggtgccggg
    21601 ctggcgctag cgaaatgggg gctggggctg aaacaggtcg tgatggcagc actgtttgtg
    21661 gccctgtttc tgccctttgg gcgcgcgcaa gaactttctc tcgcctgcct gctgacttca
    21721 cttgtcgtta cgctgctcaa ggttttgctg atttttgtac tggcctcaat cgcagaaaac
    21781 acgctggcac gcgggcgttt tttactcatt caccatgtga cctggcttgg cttcagcctt
    21841 gctgcgcttg catgggtctt ttggttaacc ggtctgtaag gagcactgac ggaatatgga
    21901 aaatcttgct ctgacgacgt taatgctgcc ttttatcggc gcactggtcg tttcgttttc
    21961 gccacaacgt cgggccgccg aatggggggt tttgttcgcc gcgctgacca cgctgtgcat
    22021 gttgtcgctg atctccgcgt tttatcaggc cgataaagtt gccgtcacgt tgacgttggt
    22081 caacgtgggg gatgtggcat tatttggcct ggtcattgat cgcgtgagta cgctgattct
    22141 gtttgtggtg gtgtttctcg gtttgctggt cacgatctac tccacgggtt atctgacgga
    22201 taaaaatcgc gaacacccgc ataacggcac gaatcgttat tacgcatttt tgctggtgtt
    22261 tatcggcgcg atggcgggac tggtactctc ctcgacgctg ctcggtcagt tgttgttttt
    22321 tgaaattacg ggcggctgct cctgggcgtt gatcagttat taccagagcg ataaagcgca
    22381 gcgttcagca ctaaaagcgt tgcttatcac ccatatcggt tcgttggggt tgtatcttgc
    22441 cgccgccacg ctgtttttgc agaccggaac gtttgtgctt agcgcgatga gcgagttaca
    22501 tggcgacgca cgttatctgg tttatggcgg catcctgttt gccgcgtggg ggaaatcggc
    22561 ccagctaccg atgcaagcgt ggctaccgga cgcaatggaa gcgccaacac cgatcagcgc
    22621 ctatctccac gccgcatcga tggtgaaagt gggcgtttac atttttgccc gcgccattat
    22681 cgacggcggc aatatcccgc atgtgattgg cggcgttggc atggtcatga cactggtcac
    22741 cattctttat ggctttctga tgtatttgcc acagcaggat atgaagcggt tgctggcctg
    22801 gtcgaccatc actcaacttg gctggatgtt cttcggcttg tcgctctcca tcttcggctc
    22861 gcggctggcg ctggagggta gcatcgccta catcgtcaac cacgcgttcg ctaaaagcct
    22921 gtttttcctt gtagcaggtg cgctgagtta cagctgcggc acgcgcttgt tgccgcgtct
    22981 gcgtggcgta ttgcacaccc tgccgttgcc aggcgtgggt ttctgcgtgg cagcgctggc
    23041 aattaccggt gtgccgccgt tcaacggctt cttcagtaaa ttcccgctgt ttgccgccgg
    23101 ttttgcgttg tcagtggagt actggatcct gctgcccgcc atgattctgc tgatgattga
    23161 atcggtcgcc agtttcgcct ggtttattcg ctggtttggt cgcgtcgtgc cgggcaaacc
    23221 gagcgaggcc gtcgccgatg ccgcaccgct gccagggtca atgcgcctgg tgttgattgt
    23281 actgattgtg atgtcgctga tttccagcgt aatcgccgcg acctggttgc agtaaggaga
    23341 tgatgaatga ccggttctat gatcgtaaat aatctggcgg gactgatgat gctgacatcg
    23401 ctgtttgtga ttagcgtcaa aagctatcgc ctgtcatgcg gattttacgc ctgccagtcg
    23461 ctggtgctgg tgtctatttt cgccactctc tcgtgcctgt tcgccacaga gcaactgctg
    23521 atctggtccg ccagcgcctt tatcaccaaa gtgctgctgg taccgttaat catgacttac
    23581 gctgcacgaa atattcccca gaacatcccg gaaaaagcgt tattcggtcc ggcaatgatg
    23641 gcactgctcg cggcgttaat tgtcctgctt tgcgcatttg tcgttcagcc cgtgaagcta
    23701 ccgatggcta ccgggctgaa accggcgctg gcggtagcgt taggtcattt tctgcttggc
    23761 ctgctgtgca ttgtcagcca acgcaatatc ctgcggcaaa tttttggtta ctgcctgatg
    23821 gaaaacggct cccatctggt gctggcgctt cttgcctggc gagcaccgga actggtggaa
    23881 ataggtatcg ctaccgacgc catcttcgcc gtcattgtga tggtgttact ggcaagaaaa
    23941 atatggcgta cccacggcac gctggacgtg aacaacttga ccgcgctgaa gggataatga
    24001 gatgagttat tctgtgatgt tcgctttact cctgctcacg ccgctgcttt tttcgctgct
    24061 ctgttttgcc tgccggaaac gggggctctc tgcgacttgt acggtgactg tattacatag
    24121 cttagggatc acactgctgc tgattctagc actctgggtg gtccaaactg ccgctgatgc
    24181 aggagaaata ttcgctgcgg gactgtggct tcatattgat ggtctgggcg gtttgttcct
    24241 cgccattctt ggtgtgattg gctttctcac cggtgtttac tcgattggct acatgcgtca
    24301 tgaagtggca cacggcgagc tttcacccgt tacactgtgc gattactacg gtttcttcca
    24361 tctgtttttg ttcaccatgc tgctggttgt taccagcaat aacctgattg tgatgtgggc
    24421 ggcgatcgaa gccaccacct taagctcggc gtttctggta ggcatttacg gtcagcgttc
    24481 atcgctggaa tcagcatgga agtacatcat tatttgtact gttggtgtcg cttttggtct
    24541 gttcggtacc gtgctggtat acgccaacgc cgccagcgtt atgccgcagg cagaaatggc
    24601 gatattctgg agcgaggttc ttaagcaatc gtccttgctt gacccaacat taatgctgtt
    24661 ggcctttgtg tttgtgctaa ttggctttgg taccaaaacc gggctatttc ccatgcacgc
    24721 ctggctgccg gatgctcaca gtgaagcgcc gagtccggtc agcgccctgc tctccgccgt
    24781 attgctgaac tgcgcgctgt tggtgctgat tcgctattac atcattattt gccaggccat
    24841 cggcagcgat ttccccaacc ggttgttgct catcttcggc atgttgtcgg ttgccgtggc
    24901 ggcatttttc attctggtac agcgggacat taagcgtctg ctggcgtact ccagcgtgga
    24961 gaacatgggg ctggtcgcgg tggcgctagg cattggcggg ccgctgggaa tttttgccgc
    25021 gctgctgcac accttaaacc acagtctggc aaaaacgctg ctgttctgcg gttccggcaa
    25081 tgtactgctc aagtacggca cgcgcgatct caacgtcgtc tgtgggatgc tcaaaatcat
    25141 gccatttacc gccgtgctgt ttggcggcgg tgcgctggcg ctggcgctgg cagggatgcc
    25201 gcccttcaac atttttctta gcgaatttat gaccgttacc gcaggactgg cacgtaatca
    25261 cctgctgatt atcgtcctgc tgttattgct gttaacgctg gtgctggcgg gcctggtacg
    25321 gatggctgcg cgggtgttaa tggcgaaacc gccgcaggcc gttaaccggg gtgatctcgg
    25381 ctggttgacc acctcgccaa tggtgattct gctggtcatg atgctggcga tgggaacgca
    25441 tattccacaa cctgtcatca ggatcctggc gggcgcttcc actatagtcc tctcagggac
    25501 gcacgacctg cctgcacaac gtagcacctg gcatgatttt ttgccttcag gcaccgcatc
    25561 tgtttcggag aaacacagtg aacgttaatt catcgtcaaa tcgtggcgaa gcgattctcg
    25621 ccgccctgaa aacgcagttc cccggcgcgg tgctggatga agagcgacaa acgcctgaac
    25681 aggtcaccat tacggtgaaa atcaatctgc tgcctgacgt tgtacattat ctttattatc
    25741 aacatgatgg ctggcttcca gtcctgtttg gcaacgacga gcggacactt aacggtcatt
    25801 acgcggttta ttatgccctt tcaatggaag gggccgaaaa atgctggatc gtggtgaagg
    25861 cactggtcga tgccgacagt cgggagtttc cgtcagtcac accgcgcgtc cctgccgcgg
    25921 tctggggcga gcgagaaatt cgcgatatgt acgggctgat tccggttggc ctgccggatc
    25981 agcgtcgcct ggtgttgccc gatgactggc cggaagatat gcatccgctg cgcaaagatg
    26041 cgatggatta tcgactgcgc cctgaaccga cgacagattc cgaaacgtat gcgtttatca
    26101 atgagggcaa cagcgatgcg cgggtgatcc ctgtcggccc gctgcatatc acctcctatg
    26161 aaccgggtca cttccgcttg tttgtggatg gcgagcaaat tgtcgatgct gattaccgcc
    26221 tgttttatgt ccatcgcggc atggagaaac tggcagaaac gcggatgggc tacaacgaag
    26281 tgaccttctt atcggaccgc gtgtgtggga tttgcggttt tgcccacagt gtggcctata
    26341 ccaactcggt tgaaaatgca ctggggattg aggtgccgca acgagcacat actattcgct
    26401 cgattctgct ggaagtcgag cggctacaca gtcatttgct caaccttggc ctctcctgcc
    26461 attttgttgg ttttgatacc ggctttatgc aatttttccg cgtgcgggaa aagtcgatga
    26521 cgatggcgga attgctgacc gggtcgcgta aaacctacgg tctgaatctg attggtggtg
    26581 ttcgccgcga tattctcaaa gagcaacgtc tgcaaacgct gaaactggtg cgcgagatgc
    26641 gcgccgacgt gtcggagctg gtagagatgc tgcttgctac gccgaatatg gaacaacgca
    26701 ctcagggcat tggcattctc gaccgacaaa tcgcccgtga ttatagccct gtagggccgc
    26761 tgatccgcgc agtggttttg cccgtgattt gcgctttgat cacccctacg ccgactacgg
    26821 caatattcca aaaacactgt ttacctttac cggcggcgat gttttctccc gcgtgatggt
    26881 ccgtgtcaaa gagacgtttg attcgctggc aatgctggaa tttgccctcg acaacatgcc
    26941 ggatacccca ctgctgaccg aaggctttag ctataaacct cacgcattcg cgctgggctt
    27001 tgttgaagcg ccacgcggtg aagacgtgca ctggagcatg ctcggtgata accaaaaatt
    27061 gttccgctgg cgctgtcgtg ccgccaccta cgccaactgg ccggtgttgc gttacatgct
    27121 gcgcggcaat accgtttctg acgcaccgct gattatcggt agccttgatc cctgctactc
    27181 ctgtaccgac cgtgtgacgc tggtagatgt gcgcaagcgc cagtcaaaaa ccgtgctgta
    27241 taaagagatc gaacgctacg gcattgatcg taaccgttcg ccgctgaagt aaggacagaa
    27301 gatgctgaag ttactgaaaa ctattatgcg cgccggaacc gcgacggtga aatatccctt
    27361 cgcgccactg gaggtcagcc caggctttcg cgggaaaccg gacctgatgc ccagccaatg
    27421 tattgcctgc ggtgcctgcg cctgtgcttg tccggcaaat gcgctgacta tccagaccga
    27481 cgaccagcaa aattcgcgca cctggcagct ctatctgggg cgttgtattt actgcggacg
    27541 ttgtgaagaa gtgtgcccga ccagagccat ccagcttacc aataactttg aactgaccgt
    27601 cacccataaa gccgatctct atacccacgc gacgttccat ctacaacgtt gcagccgttg
    27661 cgaacgcccg tttgccccgc aaaaaaccgt cgcactggct gctgaattgt tggcacagca
    27721 acaaaatgct ccacaaaacc gcgaaatgtt atgggcgcaa gcgagcgtct gcccggagtg
    27781 caaacaacgc gcaacgctga tcaacgacga tacagatgta ccgctggtgg ctaaggagca
    27841 gctatgagtc cagtgcttac gcaatatgtc agccagccca tcacgctgga cgagcaaacg
    27901 caaaagatga agcggcattt gctacaggat atccgtcgct cggcttacgt ttatcgcatc
    27961 gattgcggcg gctgcaacgc ctgtgaaatc gaaatttttg ctgccatcac ccccgtattc
    28021 gacgcagaac gttttggcat taaggttgtt tcatcaccgc gtcacgccga tattttgtta
    28081 tttactggcg cagtcacccg ggcgatgcgt atgcctgcac ttcgggcgta tgagtctgcc
    28141 cccgatcata aaatctgtgt ttcctacggc gcgtgcggtg tcggcggcgg tattttccac
    28201 gatctctaca gcgtctgggg cggcagcgac accattgtcc ccattgatgt ttggatcccc
    28261 ggctgcccgc caacaccggc cgccaccatt cacggtttcg ccgtggcgct cggtttgctg
    28321 caacagaaga ttcacgcggt ggattatcgc gatcccaccg gggtgactat gcaaccgttg
    28381 tggccgcaga tcccgccatc acagcgtatc gccattgagc gagaagcgcg gcggctggcg
    28441 ggctatcgtc aggggcgaga aatttgcgat cggctcctgc gccatttaag cgacgatcct
    28501 acaggaaatc gggttaacac ctggttgcgc gatgccgacg atccacgtct caatagtatc
    28561 gttcagcaac tctttcgcgt actccggggg ttacatgact gaagagtgcg gggaaattgt
    28621 tttctggacg ctgcgaaaaa agtttgtcgc cagtagcgac gagatgccgg aacacagctc
    28681 tcaggtaatg tattactcgc tggctatcgg tcatcacgtt ggcgtgattg attgtctgaa
    28741 tgtcgccttc cgctgcccac tgacggaata cgaagattgg cttgcactgg tcgaagagga
    28801 gcaagcccga cgtaagatgc tgggggtgat gacttttggt gagattgtta ttgacgccag
    28861 ccacaccgcc ctgttgaccc gggcattcgc gccactggca gatgacgcga cgtctgtgtg
    28921 gcaagcgcgt agcattcaat tcattcatct gttggatgaa attgtgctgg aaccggctat
    28981 ctatctgatg gccagaaaaa ttgcgtgaga aggatttctc attaataagg actgttgatg
    29041 gctatgtcag acgaagcgat gtttgccccg ccgcaaggaa taacaattga agcggtaaac
    29101 ggaatgctcg cggagcggtt agcgcagaaa cacggcaagg cgtctttatt acgcgccttc
    29161 atcccgctgc cgccgccgtt cagcccggta caacttattg aactgcatgt tctcaaaagc
    29221 aacttctatt accgctacca tgatgatggc agcgatgtga cggcaacaac agagtatcag
    29281 ggcgagatgg tcgattattc gcgttacgcc gtccttctcg gcagtagtgg aatggcggag
    29341 ctacgcttta ttcgtaccca cggcagccgt tttactcccc aggattgcac actgtttaac
    29401 tggctggcac gcattatcac cccggttctg caatcatggc tcaatgatga agcacagcag
    29461 gtggcgctgc gtttgctgga gaaagatcgc gatcatcatc gggtactggt ggatatcact
    29521 aatgcagtgc tgtcacatct tgatctcgac gatctgatcg ctgacgtcgc tcgtgagatc
    29581 catcattttt tcggtctggc ttcagtcagt atggtactgg gcgatcatcg aaagaacgag
    29641 aagttcagcc tgtggtgcag cgatctttct gcctcacatt gtgcgtgtct gccacacaat
    29701 atgcctggcg acagtgtatt gctgacacaa acgctacaaa cccgacaacc gaccttgacg
    29761 caccgtgcag atgatctgtt tctctggcaa cgcgacccgt tattactctt acttgcatct
    29821 aacggctgcg aatctgcgct ccttataccg cttacctttg gcaaccatac accgggtgca
    29881 ttgttgctgg cgcatacctc ttccactctc tttagtgagg aaaactgcca gctactacaa
    29941 cacatagccg atcgcatcgc tattgccgtt ggcaatgccg atgcctgccg tagaatgacc
    30001 gatttgcagg aaagtttgca gcaagaaaac caccagctta gcgagcagct cctttcgaat
    30061 ctgggcgtcg gtgacattat ctatcaaagc caggcaatgg aagacctgct ccagcaggta
    30121 gatattgtgg cgaagagcga cagtacggtg ttgatttgcg gtgaaaccgg aaccggcaaa
    30181 gaggtgatcg ccagagcgat ccatcaactt agcccgcgac acgacaagcc gctggtcaaa
    30241 atcaactgcg ctgccatccc cgccagtctt ctggaaagtg agttattcgg tcatgacaaa
    30301 ggggcgttta ctggtgcgat taatacccat cgtggtcgtt ttgaaattgc cgatggcggc
    30361 acgttgtttc tcgatgaaat tggcgatctg ccgttagaac ttcagcctaa actgctgcgc
    30421 gtattgcagg aacgggagat tgagcgtctc ggcgggagta gaacgatccc ggtaaatgtc
    30481 agagtcattg ccgccaccaa ccgtgatttg tggcaaatgg ttgaagatcg ccagtttcgc
    30541 agcgatctct tttatcgcct gaatgtcttc ccactggaat tgcctccgct gcgcgaccgt
    30601 ccggaagata tccctctttt agcaaagcat ttcacgcaaa aaatggcgcg ccatatgaat
    30661 cgttcaattg acgccatccc gaccgaggcg ctacgccagt tgatgtcgtg ggactggccg
    30721 ggcaacgtgc gcgagctgga aaacgtgatt gagcgagcgg tactgctgac tcgcggtaac
    30781 agtctgaatt tacatcttaa tgtccgacaa agccgtttac tgccgacgct aaatgaagat
    30841 tcagcgcttc gcagttcaat ggcgtagttg ctgcacccga cgacgccaga gaatgacgaa
    30901 gaagaacgtc agcgcattgt tcaggtattg cgagaaacca atggcattgt tgccgggccc
    30961 cgtggcgcgg cgacacgatt agggatgaag cgcaccacgc tgctgtcacg aatgcagcgt
    31021 ctgggaatct cggttcgcga ggtgttgtaa tctgcttttg caggagtatg catgagaaac
    31081 aaactctctt tcgacttgca gttgagcgcc agaaaagcgg caatcgctga acggattgcc
    31141 gcccataaaa ttgcccgcag taaagtgtcg gtctttttaa tggcgatgtc cgctggcgtg
    31201 tttatggcga tgtccgctgg cgtgtttatg gcgatcggat ttacttttta cctttccgtt
    31261 atcgccgatg ccccgtcttc ataggcatta acccatctgg tgggcggcct ttgctttaca
    31321 cttggcttta ttttgctggc ggtttgcggc accagcctgt tcacctcgtc ggtaatgacg
    31381 gtaatggcaa aaagtcgggg cgttattagt tggcgaactt ggctgattaa cgcacttctg
    31441 gtggcctgcg gtaatctggc aggtattgcc tgtttcagtt tgttaatctg gttttccggg
    31501 ctggtgatga gtgaaaacgc gatgtgggga gtcgcgattt tacactgcgc cgagggcaaa
    31561 atgcatcata catttactga atctgtcagc ctcggcatta tgtgcaatct gatggtttgc
    31621 ctggcgctgt ggatgagtta ttgcggacgt tcgttatgcg acaaaatcgt cgccatgatt
    31681 ttgtccatca ccctgtttgt cgccagtggc tttgagcact gtatcgccaa tttgtttgtg
    31741 attccgttcg ccattgccat tcgccatttc gcccctacct ccttctggca actggcgcac
    31801 agtagcgcag acaattttcc ggcactgacg gtcagccatt ttattaccgc caatctgctc
    31861 ccggtgatgc tgggtaatat tatcggcggt gcagtgctgg tgagtatttg ttatcgggct
    31921 atttatttac gtcaggaatc ctgaaaaatc agcccggcga aacagttcgt cgggctgaag
    31981 attattcttg cgcgatttgc ccatcgggcc aggcgtggat cacggctttg atcagcgtcg
    32041 ccaatggaat ggcgaagaat acgccccaga atccccacaa accaccgaag atcaccaccg
    32101 ataaaataat caccagcgga tgcaggttaa ccgcttcgga gaacaacacc ggtactagca
    32161 ggttgccgtc cagcgcctga ataatcagat acaccgcgaa gcagctccag aattcagtac
    32221 ctgcgccaaa ctggaatagc gccacgccaa ccaccggaat ggtcaccaca aatgcgccaa
    32281 tgtacggaat aagaaccgag aagccgacca gcactgccag cagcagcgaa tagttcagcc
    32341 cgaagagcaa gaaccccagc caggtggcga tccccaccac gatcatctcc agcactttgc
    32401 cgcggatata gttggtgatt tgttgattca tctccttcca cacctgtcct gccagcccac
    32461 ggttacgcgg cagcacccgg cgaacggcgt tcagcatctg ctctttgtct ttcagcagga
    32521 agaagaccat caatggcacc agcaccagat agacggctat ggtcagcaaa ccgaccagcg
    32581 aggcgaggga aattttcacc accgaatcgc ccatggtcag catccgactg cgcatatttt
    32641 cggccattgc atcaataatg cccgcgtcca ttaacaccgg atagcggcgc ggcaacgtgg
    32701 cggcaaagtc agaaagctta ttgagcatcc ccggcatatc gcggattaag tagatgccct
    32761 gttgccaggc gataggcagt accacgaacg ccatcagtag caatataccg acaaaaacca
    32821 ccaatacaat cgacgtcgcc cagcggcggg agcagccaat agattgcagg cgcacggttg
    32881 gccattccag caaataagcc agcacaatag ccaccagcaa cggagcaagc aggccactaa
    32941 agaaaaagat aatgccaaat ccggcaacta aaataaccag caaggcaatc gcttccgggt
    33001 cgctaaaacg gcggcgatac cattgcatca acatttcgag cataaagacc ttccctgaac
    33061 ctcaagagcg ggattgcgat ccgcaattgt atcgaaatgt cacaaaaaag acttcgcttt
    33121 ttatgacgga ttcaggaaac tgaaaagtca tttgagtggg ctaatcttcg ccgttacact
    33181 caaaggcggc gaggtgggaa cgatatttca cagtatcggt caaatgacta tcttcagaaa
    33241 tacaggatag aggttatgtt caggcagttg aaaaaaaacc tggttgcaac cctcattgct
    33301 gctatgacca ttggtcaggt agccccggcg tttgccgaca gcgcagacac cttgccggat
    33361 atgggaacct ccgcaggaag cacgctttcc attggtcagg aaatgcagat gggcgactat
    33421 tatgtccgcc agctacgcgg cagcgcgccg ttaattaatg acccgctgtt aacgcaatat
    33481 attaattcgc tggggatgcg tctggtttcg catgccaatt cggttaagac accgtttcat
    33541 ttttttctga tcaacaacga cgaaattaac gcctttgctt tctttggcgg caacgtggtg
    33601 ctgcactctg ccctgttccg ttattccgat aacgaaagtc aactggcttc agttatggcg
    33661 cacgaaatct cccacgtcac ccaacgtcac ctggcgcgag cgatggaaga tcaacaacgc
    33721 agcgcgccgc tgacctgggt cggcgcgtta ggttctattt tactggcgat ggccagtccg
    33781 caggcgggga tggcggcgct gaccggtaca ctggcgggaa cgcgccaggg gatgatcagt
    33841 ttcacccagc aaaatgaaca ggaagcggac cgcattggta ttcaggtgct gcaacgctcg
    33901 ggattcgatc cacaggcgat gccgaccttc ctggaaaaat tgctcgatca ggcgtgttac
    33961 tcctcgcgcc cgccggaaat tttattgact cacccgttgc cggaaagtcg tctggcagat
    34021 gcccgcaacc gtgctaatca gatgcgcccg atggtggtgc agtcgtcgga agatttctat
    34081 ctggcgaaag cgcgcacact ggggatgtat aattccggac gtaaccagct caccagtgat
    34141 ttgctggatg aatgggcgaa aggaaacgtt cgtcagcaac gagcggcgca atatggtcgt
    34201 gctttacagg cgatggaagc caataaatac gacgaggcgc gtaaaacgct gcaaccgtta
    34261 ctggcggcag aacctggcaa cgcatggtat ctcgatctgg ctaccgatat cgatcttggg
    34321 caaaacaaag ccaatgaggc aatcaatcgc ctgaaaaatg cccgcgattt acgcaccaat
    34381 ccggtgttgc agctcaacct ggcgaacgct tatctgcaag gcggtcaacc acaagaagcg
    34441 gccaatattc tgaatcgcta cacctttaat aataaagatg acagcaacgg ctgggatttg
    34501 ctggcacagg cggaagccgc gctaaataac cgcgatcagg agctggctgc gcgagcagaa
    34561 ggttatgcgc tcgccggacg actcgatcag gccatttcgc tgttgagtag cgccagttcg
    34621 caggtgaaat taggcagcct gcaacaagcg cgttacgatg cgcgcatcga ccagttgcgc
    34681 cagctgcaga aacgctttaa gccttatacc aagatgtaat tcgcattaaa ggaagaacaa
    34741 tgaccaaaca ggtaaaaatc taccataacc cacgctgttc aaagagccgg gaaacgctga
    34801 acctgctgaa agaaaacggc gtggagccag aagtggtgct ctaccttgag acacccgccg
    34861 atgcggcaac gctgcgcgat ttgctgaaaa tgctggggat gaatagcgcc cgtgaattga
    34921 tgcgccagaa agaggatctt tataaagaac taaacctggc tgacagttcg cttagcgaag
    34981 aggcgctgat tcaggcaatg gtggataatc cgaagctgat ggagcgcccg attatggtcg
    35041 cgaatggcaa agcgcggatt ggtcgtccac cggagcaggt actggaaatc gtgggttaag
    35101 atgtgctcca tcgctagttg aagcacattg ccggatgcga cgcttgccac gtcttatccg
    35161 gcctacgaat actatggatt aacttcgcgt aggtcaggcg ttcgcgccgc atccgacaat
    35221 aaacacctta tctacaactt cagaatttct ttcacaaacg gaatggtcag cttacgttgc
    35281 gcggtaatcg acgcacgatc caactgatcc aacgtcataa atagcgtgcg catttctctg
    35341 tcgagccgct tcagcaagaa acgccccaca tcttccggca gttcaaaacc acgcaaacgc
    35401 gcgcgtaact gtagcgcctg caacttatct tcatcagaaa gtggctgcaa tttgtagatc
    35461 tgcccccagt cgagacgcga cgcgagatcc ggtaatccca gattcaactg ccgcggtgga
    35521 cgatcgccgg tgatcaacaa ccgtgttttg cccgattcca gaattcgatt gtagagatcg
    35581 aaaatcgcca tctcccacaa ctcatcgcct gcaatacact caatgttgtc gatacagacc
    35641 agcgacaaat gctccatacc gtcgagcact tccggaacaa accaggtgcg tttatccagc
    35701 gggacatagc ccaccgcatc gccacgctgc gacaattccg cgcaagccgc gtgcagcaga
    35761 tggctgcgcc ccgcgccttc gcgtgcccag agatagatgt aaccgctatg ttcctgacgc
    35821 agcacgtttt gcagcgcggc cagtaaagag gagttatccc ccggccagaa acttgcaaag
    35881 gtttcgtcgt caggaagata aagtggcaaa gagagctgtg ccggtgtgtt cagagatacc
    35941 tcaaccagga tttcacaaaa tcgcgagaag tttaccacag aatcccatga tgtttgaacc
    36001 gggcagcaac actgcccggt cggtacatta tttgtctgtt atatccgcgt cttctgcgtc
    36061 cagcaccact tcttcagggc gcagcacact gatcagtttg aaaatcaaac ttaagccgat
    36121 accgacgatg gtcgccagcg ccataccttt taactccgcc gcgccgatgt ttaccttcgc
    36181 gccactgacg ccgatgatca aaatcacgga agtcaggatc aggttctgtg ctttgttgta
    36241 atccactttc gattcgatca aaacacgaat accggaagca ccaatgacac cataaagcag
    36301 cagcgaaacg ccgcccatca ccggcaatgg gatcatctgg atagcggcag ccagtttacc
    36361 gacgcaggaa agcaggatag cgaaaatcgc cgccccgccg ataacccagg tactgtaaac
    36421 acgggtgatc gccatcacgc caatgttttc tccgtaagtg gtatttggcg tagagccaaa
    36481 gaagccggag atcacggtcg acaggccgtt agcaaacatc gaacggtgca gacctggatc
    36541 gcgcagcaga tcttttttga cgatattagc cgttactacc aggtgcccta cgtgttcggc
    36601 aataaccact aacgccgctg gcagaatagt cagaatggca aaccactcga agcgcggcgt
    36661 atagagggtt ggcagtgcaa accagtgagc attaataatc ggcgtggtat cgacaattcc
    36721 cattgcgaaa gagagcgcgt accccaccag cacgccaatt aaaatcggga taattgccag
    36781 gaaaccacga aacagcacgg aacctaaaac cgtgaccgcc agggtggtaa tagagatgat
    36841 gatggttttg gagtttggcg tttgcccttc agccgggagt aaacccgcca taccggcagc
    36901 tacgcccgcc agctccagac cgatgacggc aacgattgcg cccattgccg caggtggaaa
    36961 cagcacgtcc aaccagccgg tccccgcttt cttcacgata aaagaaacca ggcagaacag
    37021 cacgccgcac ataataaagc cgcccagcgc gacttcatac cctaacggca acagtaacaa
    37081 taccggtgaa ataaaggcaa agctggaacc aagataagcc ggaattttcc ctttacagat
    37141 gaagagatac agcagcgttc caataccgtt aaataacagt acagtcgccg ggttaatatg
    37201 aaataagacg ggcaccagga cggttgcacc aaacatggcg aacaaatgtt gcaaactaag
    37261 cgggattgtc tgtaaaagtg gcggtctttc actcaccccg atagcacggc gcgtcatagt
    37321 attatcctct gtattatgtg ttataggcgc tttactcaaa aaaaagccga ctcttaaagt
    37381 cggctttaat tatttttatt ctttatttcg taccaaagat tttgtcaccg gcatcgccga
    37441 ggcccggaat aatgtatccg tgctcgttca gtccctggtc aatcgatgca gtatacagtt
    37501 cgacgtccgg gtgcgctttt tccagcgcag cgataccttc tggcgcagcg accagcacca
    37561 gaactttgat gctgctgcag cccgcttttt tcagcaggtc gatggtcgcg ataacggaac
    37621 caccggttgc cagcattggg tcaacgatca gcgccatacg ctcatcgatg ttagaaacca
    37681 gtttctggaa gtacggtacc ggctccagcg tttcttcatt acggtacata ccgacaacgc
    37741 tgatgcgcgc gctcggaacg ttttccagca caccgtccat cataccaaga cccgcacgca
    37801 gaattggcac aacggtaatt ttcttacctt tgatctggtc gatttctacc gggccgttcc
    37861 agccttcgat agttactttt tccgtttcga ggtcggcggt cgcttcgtaa gtcagcaggc
    37921 tacccacttc ggaagcgagt tcgcgaaagc gcttggtgct gatatcttgc tcacgcatca
    37981 gtcccagctt gtgtttgacg agtgggtgtt tgacttccac gatcttcata ctctttctcc
    38041 tttgaggggc agccacaaaa aaatcgacgg attatacctc ctttcttcag ggcggcaata
    38101 ttattttcgt tgactttagt caaaatgata acagtttgag ataaagttat tttatattca
    38161 aatggttatg aaagaagatt attccatccg aaaactaacc tttgccctgg cacaagtctt
    38221 ctttcgccgc gcgcccgggg aaaagacgtg caaaaaggtt gtgtaaaaca gtctcgcaaa
    38281 cgtttgcttt ccctgttaga attgcgccga attttatttt tctaccgcaa gtaacgcgtg
    38341 gggacccaag cagtgaccga taaaacctct cttagctaca aagatgccgg tgttgatatt
    38401 gacgcgggta atgctctggt tggaagaatc aaaggcgtag tgaagaaaac gcgtcgtccg
    38461 gaagtgatgg gcggtctggg cggcttcggt gcgctgtgtg cattgccgca aaaatatcgt
    38521 gaacccgtgc tggtttccgg cactgacggc gtaggtacca agctgcgtct ggcaatggac
    38581 ttaaaacgtc acgacaccat tggtattgat ctggtcgcca tgtgcgttaa tgacctggtg
    38641 gtgcaaggtg cggaaccgct gtttttcctc gactattacg caaccggaaa actggatgtt
    38701 gataccgctt cagcggtgat cagcggcatc gcggaaggtt gtctgcaatc gggctgttct
    38761 ctggtgggtg gcgaaacggc agaaatgccg gggatgtatc acggtgaaga ttacgatgtc
    38821 gcgggtttct gcgtgggcgt ggtagaaaaa tcagaaatca tcgacggctc taaagtcagc
    38881 gacggcgatg tgctgattgc actcggttcc agcggtccgc actcgaacgg ctattcgctg
    38941 gtgcgcaaaa ttcttgaagt cagcggttgt gatccgcaaa ccaccgaact tgatggtaag
    39001 ccattagccg atcatctgct ggcaccgacc cgcatttacg tgaagtcagt gctggagttg
    39061 attgaaaagg tcgatgtgca tgccattgcg cacctgaccg gcggcggctt ctgggaaaac
    39121 attccgcgcg tattgccaga taatacccag gcagtgattg atgaatcttc ctggcagtgg
    39181 ccggaagtgt tcaactggct gcaaacggca ggtaacgttg agcgccatga aatgtatcgc
    39241 accttcaact gcggcgtcgg gatgattatc gccctgcctg ctccggaagt ggacaaagcc
    39301 ctcgccctgc taaatgccaa cggtgaaaac gcgtggaaaa tcggtatcat caaagcctct
    39361 gattccgaac aacgcgtggt tatcgaataa tgaatattgt ggtgcttatt tccggcaacg
    39421 gaagtaattt acaggcaatt attgacgcct gtaaaaccaa caaaattaaa ggcaccgtac
    39481 gggcagtttt cagcaataag gccgacgcgt tcggccttga acgcgcccgc caggcgggta
    39541 ttgcaacgca tacgctcatc gccagcgcgt ttgacagtcg tgaagcctat gaccgggagt
    39601 tgattcatga aatcgacatg tacgcacccg atgtggtcgt gctggctggt tttatgcgca
    39661 ttctcagccc ggcgtttgtc tcccactatg ccgggcgttt gctgaacatt cacccttctc
    39721 tgctgccgaa atatcccgga ttacacaccc atcgtcaggc gctggaaaat ggcgatgaag
    39781 agcacggtac atcggtgcat ttcgtcaccg atgaactgga cggtggcccg gttattttac
    39841 aggcgaaagt cccggtattt gctggtgata cggaagatga catcaccgcc cgcgtgcaaa
    39901 cccaggaaca cgctatttat ccactggtga ttagctggtt tgccgatggt cgtctgaaaa
    39961 tgcacgaaaa cgccgcgtgg ctggatggtc aacgtctgcc gccgcagggc tacgctgccg
    40021 acgagtaatg cccccgtagt taaagcgcca gctctgccgc tggcgttttt caattcacct
    40081 gtaaatcgca agctccagca gtttttttcc cccttttctg gcatagttgg acatctgcca
    40141 atattgctcg ccataatatc caggcagtgt cccgtgaata aaacggagta aaagtggtaa
    40201 tgggtcagga aaagctatac atcgaaaaag agctcagttg gttatcgttc aatgaacgcg
    40261 tgcttcagga agcggcggac aaatctaacc cgctgattga aaggatgcgt ttcctgggga
    40321 tctattccaa taaccttgat gagttctata aagtccgctt cgctgaactg aagcgacgca
    40381 tcattattag cgaagaacaa ggctccaacg ctcattcccg ccatttactg ggcaaaattc
    40441 agtcccgggt gctgaaagcc gatcaggaat tcgacggcct ctacaacgag ctattgctgg
    40501 agatggcgcg caaccagatc ttcctgatta atgaacgcca gctctccgtc aatcaacaaa
    40561 actggctgcg tcattatttt aagcagtatc tgcgtcagca cattacgccg attttaatca
    40621 atcctgacac tgacttagtg cagttcctga aagatgatta cacctatctg gcggtggaaa
    40681 ttatccgtag cgatagcatc cgttacgcgc tgctggagat cccatcagat aaagtgccgc
    40741 gctttgtgaa tttaccgcca gaagcgccgc gtcgacgcaa gccgatgatt cttctggata
    40801 acattctgcg ttactgcctt gatgatattt tcaaaggctt ctttgattat gacgcgctga
    40861 atgcctattc aatgaagatg acccgcgatg ccgaatacga tttagtgcat gagatggaag
    40921 ccagcctgat ggagttgatg tcttccagtc tcaagcagcg tttaactgct gagccggtgc
    40981 gttttgttta tcagcgcgat atgcccaatg cgctggttga agtgttacgc gaaaaactga
    41041 ctatttcccg ctacgactcc atcgtccccg gcggtcgtta tcataatttt aaagacttta
    41101 ttaatttccc caatgtcggc aaagccaatc tggtgaacaa accactgccg cgtttacgcc
    41161 atatttggtt tgataaagcc cagttccgca atggttttga tgccattcgc gaacgcgatg
    41221 tgttgctcta ttatccttat cacacctttg agcatgtgct ggaactgctg cgtcaggctt
    41281 cgttcgaccc gagcgtactg gcgattaaaa ttaacattta ccgcgtggcg aaagattcac
    41341 gcatcatcga ctcgatgatc cacgccgcac ataacggtaa gaaagtcact gtggtggttg
    41401 agttacaggc gcgtttcgac gaagaagcca acattcactg ggcgaagcgc ctgaccgaag
    41461 caggcgtgca cgttatcttc tctgcgccgg ggctgaaaat tcacgccaaa ctgttcctga
    41521 tttcacgtaa agaaaacggt gaagtggtcc gttacgcaca catcgggacc gggaacttta
    41581 acgaaaaaac cgcgcgtctt tatactgact attcgttgct gaccgcagat gcgcgtatca
    41641 ccaacgaagt acggcgggta tttaacttta ttgaaaaccc ataccgcccg gtgacatttg
    41701 attatttaat ggtgtcaccg caaaactctc gccgtctgtt atatgaaatg gtagaccgcg
    41761 aaatcgccaa cgcgcagcaa gggctgccca gtggtatcac cctgaagcta aataaccttg
    41821 tcgataaagg cctggttgat cgtctgtatg cggcctccag ctccggcgta ccggttaatc
    41881 tgctggttcg cggaatgtgt tcgctgatcc ccaatctgga aggcattagc gacaacattc
    41941 gtgccatcag tattgttgac cgttaccttg aacatgaccg ggtttatatt tttgaaaatg
    42001 gcggcgataa aaaggtctac ctttcttccg ccgactggat gacgcgcaat attgattatc
    42061 gtattgaagt ggcgacaccg ctgctcgatc cgcgcctgaa gcagcgggtg ctggacatca
    42121 tcgacatatt gttcagcgat acggtcaaag cacgttatat cgataaagaa ctcagtaatc
    42181 gctacgttcc ccgcggcaat cgccgcaaag tacgggcgca gttggcgatt tacgactaca
    42241 tcaaatcact cgaacaacct gaataaccct atgccaatac acgataaatc ccctcgtccg
    42301 caggagtttg ctgcggtcga tcttggttca aacagttttc acatggtcat agcccgtgtg
    42361 gtagatggtg ccatgcagat tattggccgc ctgaaacagc gggtgcatct ggcggacggc
    42421 ctggggccag ataatatgtt gagtgaagag gcaatgacgc gcggtttgaa ctgtctgtcg
    42481 ctgtttgccg aacggctaca agggttttct cctgccagcg tctgtatagt cggcacccat
    42541 acgctgcgtc aggcgctgaa cgccactgac tttctgaaac gcgcggaaaa ggtcattccc
    42601 tacccgattg aaattatttc cggtaatgaa gaagcccgtc tgatttttat gggcgtggaa
    42661 catacccaac cggaaaaagg tcgcaaactg gttattgata ttggcggcgg atctacggaa
    42721 ctggtgattg gtgaaaattt cgaacctatt ctcgttgaaa gccgccggat gggttgtgtc
    42781 agctttgccc agctttattt tcctggcggg gtcatcaata aagagaattt tcagcgcgct
    42841 cgcatggcgg cagcacaaaa actggaaact ttaacctggc aattccgtat tcagggctgg
    42901 aacgttgcaa tgggcgcttc cggtaccata aaagccgccc atgaagtgtt aatggaaatg
    42961 ggcgagaaag acgggataat taccccggaa cgtctggaaa aactggtaaa agaaatttta
    43021 cgtcaccgta atttcgcatc gctgagttta ccgggtcttt ccgaagagcg gaaaacagtc
    43081 ttcgttcctg gactggcgat tttatgtggt gtgtttgatg ctttagccat ccgtgaactg
    43141 cgcctttctg acggggcgtt acgcgaaggc gtactgtatg aaatggaagg acgtttccgt
    43201 catcaggatg tgcgtagtcg caccgccagc agcctcgcca accagtatca catagacagc
    43261 gaacaggccc gacgagtgct ggataccact atgcaaatgt acgaacagtg gcgggaacag
    43321 caaccgaagc tggcgcatcc gcaactggag gcgctactgc gatgggccgc catgctgcat
    43381 gaggtcgggt tgaatatcaa ccacagcggt ttgcatcgcc actccgctta tattctgcaa
    43441 aacagtgact tgccgggttt taatcaggaa cagcagctga tgatggcgac actggtgcgc
    43501 tatcaccgta aagcgattaa gctcgacgat ctgccgcgct ttaccttgtt taagaagaaa
    43561 cagttcctgc cactgataca gctattgcgc cttggcgtat tactaaacaa tcaacgtcag
    43621 gcaaccacca caccgccaac attgacactg attactgatg acagtcactg gacactgcgt
    43681 ttcccgcatg actggtttag tcagaatgcg ctggtactgc ttgatctgga aaaggagcaa
    43741 gaatactggg aaggcgtggc tggctggcgg ttgaaaattg aagaagaaag taccccagaa
    43801 atcgccgctt aatgctttgc gggccgacga gaaatgtcgg cccgcattat tcaggcactt
    43861 tcgcgaatgg gttcgatttc attcagcgta tcaattaacg gttgcggctt accaataaga
    43921 taaccctgca tataatcgat ccccaaagag agcaccgcct cgcggatctc ttcgttttca
    43981 acgtactctg ccactacccg cattttcttc attcgcgcca gatggcaaat cgacgccaca
    44041 atctgataat ccagactatt tgacacaata ttgcggataa agctgccgtc aattttaagc
    44101 agatcggcat tcacattttt aagccgtgca tagctggcgt agccggtgcc gaaatcatca
    44161 atcgcaatct ggcagcctaa ttcctgaaga tgctgcaagg tgatttgcgc ctgcttaaca
    44221 ttggtcagag cattactttc ggtgacttca aaaataagtt gccacgcttc aatctgatat
    44281 ttagccagca actgactgac ttcaacagga aaacgagcct gacataccga ggttggagac
    44341 agattaatag caaaacggtg agcgggcatc ttcgctctgt tttcagccat aaattgcagc
    44401 gtatgctcaa tgacccacat gtcgatactc gacgataaac caaattcgtg cgcgaccggc
    44461 aagaagctac cggggccgat cagttcatca ttctcacctt tcatgcgcag aagaatttca
    44521 tggtaaacat caccacgcat accggtaatc ggctgggcca tcaggaaaaa atggttgtgt
    44581 tccagcgcct gctgtagtcg attcatcatc gcgactttat ctttcaattc gcgttgcaaa
    44641 tacattgccc cgcaacgctg catattttcc ggggcgttgg tcacgatgga aagttcggcg
    44701 accgtattta gctctcccag cagcaggtag atatgattca ctggcgagcg cacatagcag
    44761 taactgacgc caatctgcgg ttgcatcggc atcccatccc aaaagaaacg aaattgcttg
    44821 agatgactat ccagtgcggt aatgcgctcc tggtgcgatt ctgtattcag tcgcagcgcg
    44881 agatcgttac ccgaaagctg ataaacatct tcacccggtt ccagcaaggg tgacagccag
    44941 tgagaaagtt tttgctt
//
LOCUS       DAJTSI010000015        43102 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000015 DAJTSI010000000
VERSION     DAJTSI010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43102
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.015"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            119..1138
                     /gene="epd"
                     /locus_tag="OO327_000748"
     CDS             119..1138
                     /gene="epd"
                     /locus_tag="OO327_000748"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR6017399.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAQIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            1188..2351
                     /gene="pgk"
                     /locus_tag="OO327_000749"
     CDS             1188..2351
                     /gene="pgk"
                     /locus_tag="OO327_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR6017400.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            2566..3645
                     /gene="fbaA"
                     /locus_tag="OO327_000750"
     CDS             2566..3645
                     /gene="fbaA"
                     /locus_tag="OO327_000750"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR6017401.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            3836..4696
                     /gene="mscS"
                     /locus_tag="OO327_000751"
     CDS             3836..4696
                     /gene="mscS"
                     /locus_tag="OO327_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR6017402.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            4835..5470
                     /gene="argO"
                     /locus_tag="OO327_000752"
     CDS             4835..5470
                     /gene="argO"
                     /locus_tag="OO327_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR6017403.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            5563..6303
                     /locus_tag="OO327_000753"
     CDS             5563..6303
                     /locus_tag="OO327_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR6017404.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            6470..7420
                     /locus_tag="OO327_000754"
     CDS             6470..7420
                     /locus_tag="OO327_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6017405.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(7362..8840)
                     /gene="scpC"
                     /locus_tag="OO327_000755"
                     /pseudo
     CDS             complement(7362..8840)
                     /gene="scpC"
                     /locus_tag="OO327_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(8864..9649)
                     /gene="scpB"
                     /locus_tag="OO327_000756"
     CDS             complement(8864..9649)
                     /gene="scpB"
                     /locus_tag="OO327_000756"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR6017406.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(9660..10655)
                     /gene="meaB"
                     /locus_tag="OO327_000757"
     CDS             complement(9660..10655)
                     /gene="meaB"
                     /locus_tag="OO327_000757"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR6017407.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(10648..12792)
                     /gene="scpA"
                     /locus_tag="OO327_000758"
     CDS             complement(10648..12792)
                     /gene="scpA"
                     /locus_tag="OO327_000758"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCR6017408.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(12996..13889)
                     /gene="argP"
                     /locus_tag="OO327_000759"
     CDS             complement(12996..13889)
                     /gene="argP"
                     /locus_tag="OO327_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR6017409.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            14272..14931
                     /gene="rpiA"
                     /locus_tag="OO327_000760"
     CDS             14272..14931
                     /gene="rpiA"
                     /locus_tag="OO327_000760"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR6017410.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            15187..16419
                     /gene="serA"
                     /locus_tag="OO327_000761"
     CDS             15187..16419
                     /gene="serA"
                     /locus_tag="OO327_000761"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR6017411.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            16648..16707
                     /gene="ibsC"
                     /locus_tag="OO327_000762"
     CDS             16648..16707
                     /gene="ibsC"
                     /locus_tag="OO327_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR6017412.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            17040..17099
                     /locus_tag="OO327_000763"
     CDS             17040..17099
                     /locus_tag="OO327_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6017413.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(17200..17802)
                     /locus_tag="OO327_000764"
     CDS             complement(17200..17802)
                     /locus_tag="OO327_000764"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR6017414.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(17823..18006)
                     /gene="ssrS"
                     /locus_tag="OO327_000765"
     ncRNA           complement(17823..18006)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO327_000765"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(18048..18377)
                     /gene="zapA"
                     /locus_tag="OO327_000766"
     CDS             complement(18048..18377)
                     /gene="zapA"
                     /locus_tag="OO327_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR6017415.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            18545..19123
                     /gene="ygfB"
                     /locus_tag="OO327_000767"
     CDS             18545..19123
                     /gene="ygfB"
                     /locus_tag="OO327_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR6017416.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            19149..20474
                     /gene="pepP"
                     /locus_tag="OO327_000768"
     CDS             19149..20474
                     /gene="pepP"
                     /locus_tag="OO327_000768"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR6017417.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            20471..21649
                     /gene="ubiH"
                     /locus_tag="OO327_000769"
                     /gene_synonym="visB"
     CDS             20471..21649
                     /gene="ubiH"
                     /locus_tag="OO327_000769"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR6017418.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            21672..22874
                     /gene="ubiI"
                     /locus_tag="OO327_000770"
                     /gene_synonym="visC"
     CDS             21672..22874
                     /gene="ubiI"
                     /locus_tag="OO327_000770"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR6017419.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(22948..23031)
                     /locus_tag="OO327_000771"
     CDS             complement(22948..23031)
                     /locus_tag="OO327_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6017420.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            23322..24416
                     /gene="gcvT"
                     /locus_tag="OO327_000772"
     CDS             23322..24416
                     /gene="gcvT"
                     /locus_tag="OO327_000772"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR6017421.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            24440..24829
                     /gene="gcvH"
                     /locus_tag="OO327_000773"
     CDS             24440..24829
                     /gene="gcvH"
                     /locus_tag="OO327_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR6017422.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            24948..27821
                     /gene="gcvP"
                     /locus_tag="OO327_000774"
     CDS             24948..27821
                     /gene="gcvP"
                     /locus_tag="OO327_000774"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR6017423.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            28088..28831
                     /locus_tag="OO327_000775"
     CDS             28088..28831
                     /locus_tag="OO327_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6017424.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(28888..30321)
                     /gene="bglA"
                     /locus_tag="OO327_000776"
     CDS             complement(28888..30321)
                     /gene="bglA"
                     /locus_tag="OO327_000776"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR6017425.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            30366..30676
                     /locus_tag="OO327_000777"
                     /pseudo
     CDS             30366..30676
                     /locus_tag="OO327_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            30840..31499
                     /locus_tag="OO327_000778"
     CDS             30840..31499
                     /locus_tag="OO327_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR6017426.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(31695..32675)
                     /gene="ygfZ"
                     /locus_tag="OO327_000779"
     CDS             complement(31695..32675)
                     /gene="ygfZ"
                     /locus_tag="OO327_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR6017427.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            32918..33184
                     /gene="sdhE"
                     /locus_tag="OO327_000780"
     CDS             32918..33184
                     /gene="sdhE"
                     /locus_tag="OO327_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR6017428.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMSFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            33165..33572
                     /locus_tag="OO327_000781"
     CDS             33165..33572
                     /locus_tag="OO327_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCR6017429.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(33612..34133)
                     /gene="fldB"
                     /locus_tag="OO327_000782"
     CDS             complement(33612..34133)
                     /gene="fldB"
                     /locus_tag="OO327_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR6017430.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            34245..35141
                     /gene="xerD"
                     /locus_tag="OO327_000783"
     CDS             34245..35141
                     /gene="xerD"
                     /locus_tag="OO327_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR6017431.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            35166..35876
                     /gene="dsbC"
                     /locus_tag="OO327_000784"
     CDS             35166..35876
                     /gene="dsbC"
                     /locus_tag="OO327_000784"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR6017432.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            35882..37615
                     /gene="recJ"
                     /locus_tag="OO327_000785"
     CDS             35882..37615
                     /gene="recJ"
                     /locus_tag="OO327_000785"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR6017433.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            37706..38804
                     /gene="prfB"
                     /locus_tag="OO327_000786"
     CDS             join(37706..37780,37782..38804)
                     /gene="prfB"
                     /locus_tag="OO327_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR6017434.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            38814..40331
                     /gene="lysS"
                     /locus_tag="OO327_000787"
     CDS             38814..40331
                     /gene="lysS"
                     /locus_tag="OO327_000787"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR6017435.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(40374..40922)
                     /gene="idi"
                     /locus_tag="OO327_000788"
     CDS             complement(40374..40922)
                     /gene="idi"
                     /locus_tag="OO327_000788"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR6017436.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            40977..41048
                     /locus_tag="OO327_000789"
     CDS             40977..41048
                     /locus_tag="OO327_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6017437.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(41045..41170)
                     /locus_tag="OO327_000790"
     CDS             complement(41045..41170)
                     /locus_tag="OO327_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6017438.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(41172..42620)
                     /gene="uacT"
                     /locus_tag="OO327_000791"
     CDS             complement(41172..42620)
                     /gene="uacT"
                     /locus_tag="OO327_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR6017439.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        10427 a        10790 c        11478 g        10407 t
ORIGIN      
        1 ttaaatgttc gacaattaac caatcagtcg cagtttgcga caggtaaggt ttccccggac
       61 gatttgctgg attactctgt ctggcaaatt tgctcaggga aaaccttgca ggagatctat
      121 gaccgtacgc gtagcgataa atggcttcgg tcgcatcggg cgtaatgtgg ttcgtgcttt
      181 gtatgaatcc ggacgccggg cggaaattac cgtggtggca atcaacgaac tggcggatgc
      241 tgcgggcatg gcgcatttgt tgaaatatga caccagccat ggccgttttg catgggaagt
      301 acgacaggaa cgcgatcaac tttttgttgg tgatgacgcc atccgcgtat tgcatgaacg
      361 ttcactgcaa tcgctcccct ggcgtgaact tggcgttgat gtagtcctcg actgcaccgg
      421 cgtatatggc tcccgcgagc atggcgaagc gcagattgcc gccggggcca aaaaagtgct
      481 cttttcacat cctggcagta acgatctcga cgcgaccgtt gtttacggcg tcaatcagga
      541 tcaacttcgt gcggaacacc gcatcgtttc taacgcttcc tgtaccacga attgcataat
      601 tcccgtcatc aaattgttag atgatgcgta cggtattgag tccggcactg tgaccacaat
      661 tcactccgcc atgcacgatc aacaggttat tgatgcatac catcctgacc tgcgtcgcac
      721 ccgggcagcc agccagtcga tcattccggt cgatactaaa ctggccgctg gtatcacacg
      781 attttttccg caatttaacg atcgctttga agcgattgcg gtacgtgtgc caaccataaa
      841 tgtgacggca atcgatttaa gcgtgacggt gaagaaacct gtaaaagcca atgaagtcaa
      901 cctgttgctg caaaaagcag cacaaggtgc atttcatggt atagttgact atacggaatt
      961 gccgttggtc tctgtagatt ttaaccacga tccgcacagt gccattgtcg atggcaccca
     1021 aacccgggtc agtggcgcac acctgatcaa aacgttggtc tggtgcgata acgaatgggg
     1081 ctttgctaac cgaatgctcg acacgacgtt agctatggct actgttgctt tcaggtaaga
     1141 cgcaagcagc gtctgcaaaa cttttagaat caacgagagg attcaccatg tctgtaatta
     1201 agatgaccga tctggatctt gctgggaaac gtgtatttat ccgtgcggat ctgaacgtac
     1261 cagtaaaaga cgggaaagta accagcgacg cgcgtatccg tgcttctctg ccgaccatcg
     1321 aactggccct gaaacaaggc gcaaaagtga tggtaacttc ccacctgggt cgtcctaccg
     1381 aaggcgagta caacgaagaa ttctctctgc tgccggttgt taactacctg aaagacaaac
     1441 tgtctaaccc ggttcgtctg gttaaagatt acctcgacgg cgttgacgtt gctgaaggtg
     1501 aactggttgt tctggaaaac gttcgcttca acaaaggcga gaagaaagac gacgaaaccc
     1561 tgtccaaaaa atacgctgca ctgtgtgacg tgttcgtaat ggacgcattc ggtactgctc
     1621 accgcgcgca ggcttctact cacggtatcg gtaaattcgc tgacgttgcg tgcgcaggcc
     1681 cgctgctggc agctgaactg gacgcgctgg gtaaagcact gaaagaacct gctcgcccga
     1741 tggtggctat cgttggtggt tctaaagtat ctaccaaact gaccgttctg gactccctgt
     1801 ctaaaatcgc tgaccagctg attgttggtg gtggtatcgc taacaccttt atcgcggcac
     1861 aaggccacga tgtgggtaaa tccctgtacg aagctgacct ggttgacgaa gctaaacgtc
     1921 tgctgaccac ctgcaacatc ccggttccgt ctgatgttcg cgtagcaacc gagttctctg
     1981 aaactgcacc ggctaccctg aaatctgtta acgatgtgaa agctgacgag cagatcctgg
     2041 atatcggtga tgcttccgct caggaactgg ctgaaatcct gaagaatgcg aaaaccattc
     2101 tgtggaacgg tccggttggc gtgttcgaat tcccgaactt ccgcaaaggt actgaaatcg
     2161 tggctaacgc tatcgcagac agcgaagcgt tctccatcgc tggcggcggc gacactctgg
     2221 cagcaatcga cctgttcggc attgctgaca aaatctccta catctccact ggcggcggcg
     2281 cattcctcga attcgtggaa ggtaaagtac tgcctgcagt agcgatgctc gaagagcgcg
     2341 ctaagaagta aaaaatcaca gggcagggaa acctgcactt gtttcagcgc gcttttagag
     2401 cacgcatttt ttcatactct aaataattcg agttgcagga aggcgacaag agagtgaatc
     2461 cccaggagct tacataagta agtgactggg gtgagcgaac gcagacgcag cacatgcaac
     2521 ttgaagtatt atgagtataa ggcccgacga tacaggacaa gagacatgtc taagattttt
     2581 gatttcgtaa aacctggcgt aatcactggt gatgacgtac agaaagtttt ccaggtagca
     2641 aaagaaaaca acttcgcact gccagcagta aactgcgtcg gtactgactc catcaacgcc
     2701 gtactggaaa ctgctgctaa agttaaagcg ccggttatcg ttcagttctc caacggtggt
     2761 gcttccttta tcgctggtaa aggcgtgaaa tctgacgttc cgcagggtgc tgctatcctg
     2821 ggcgcgatct ctggtgcgca tcacgttcac cagatggctg aacattatgg tgttccggtt
     2881 atcctgcaca ctgaccactg cgcgaagaaa ctgctgccgt ggatcgacgg tctgctggac
     2941 gcgggtgaaa aacacttcgc agctaccggt aagccgctgt tctcttctca catgatcgac
     3001 ctgtctgaag aatctctgca agagaacatc gaaatctgct ctaaatacct ggagcgtatg
     3061 tccaaaatcg gcatgactct ggaaatcgaa ctgggttgca ccggtggtga agaagacggc
     3121 gtggacaaca gccacatgga cgcttctgca ctgtacaccc agccggaaga cgttgattac
     3181 gcatacaccg agctgagcaa aatcagcccg cgtttcacca tcgctgcgtc cttcggtaac
     3241 gtacacggtg tttacaagcc gggtaacgtg gttctgactc cgaccatcct gcgtgattct
     3301 caggaatatg tttctaagaa acacaacctg ccgcacaaca gcctgaactt cgtattccac
     3361 ggtggttccg gttctactgc tcaggaaatc aaagactccg taagctacgg cgtagtgaaa
     3421 atgaacatcg ataccgatac ccaatgggca acctgggaag gcgttctgaa ctactacaaa
     3481 gcgaacgaag cttatctgca gggtcagctg ggtaacccga aaggcgaaga tcagccgaac
     3541 aagaaatact acgatccgcg cgtatggctg cgtgccggtc agacttcgat gatcgctcgt
     3601 ctggaaaaag cattccagga actgaacgcg atcgacgttc tgtaagtcat tcctttctgc
     3661 ttatctcaag gcccgctctg cgggtctttt tttcgccaaa agcaaggtga ttcagatgag
     3721 aaatctgtga tctatttggc aaaattatgc tttattgttt acccttgtca gactgcccgt
     3781 cataaggcgg cggagtgtat ttctccattt tgagtcagtt gaaaaggaat attgaatgga
     3841 agatttgaat gttgtcgata gcataaacgg cgcgggaagc tggctggtag ctaaccaggc
     3901 gctgctgcta agttatgcag taaacatcgt ggcggcactc gcgatcatca tcgttggttt
     3961 gattatcgcg cggatgattt ccaacgcggt gaatcgcctg atgatctccc gtaaaatcga
     4021 tgccactgtt gctgattttc tttctgcatt agtccgttac ggtattatcg cctttacgct
     4081 aatcgctgcc ctgggacgcg tgggtgtaca aacagcgtca gtcattgctg tactcggtgc
     4141 cgcaggctta gctgttggtc tggctttgca ggggtcactt tctaacctgg ccgctggcgt
     4201 gttacttgtc atgttccgcc cgttccgtgc cggagaatat gttgacctcg gcggcgtagc
     4261 cggtactgtg ctgagtgtgc agattttctc caccaccatg cgtactgcag acggtaaaat
     4321 tatcgttatt ccgaacggta aaattattgc cggaaatatt attaacttct cccgcgagcc
     4381 agttcgccgt aacgaattta ttattggcgt ggcgtatgat tccgatatcg atcaggttaa
     4441 gcagatcctg accaatatta tccagtctga agatcgcatt ttgaaagatc gcgaaatgac
     4501 tgtgcgcctg aacgaacttg gtgcatcgtc gattaatttc gtggtccgcg tctggagcaa
     4561 cagcggcgat ctgcaaaacg tgtactggga tgtgctggag cgtattaaac gtgaatttga
     4621 tgccgccggt atcagcttcc cgtacccgca aatggatgtg aactttaagc gggtgaaaga
     4681 agacaaagct gcgtaatcaa cgctgaggcc agataatact ctctggcctc tctcttatta
     4741 gttattctga ttgccaatta atattatcaa tttccgctaa taacaatccc gcgatatagt
     4801 ctctgcatca gatacttaat tcggaatatc caacgtgttt tcttattact ttcaaggtct
     4861 tgctcttggg gcagctatga ttctgccgct cggcccacaa aatgcttttg tgatgaatca
     4921 gggcattcgt cgtcagtacc acattatgat tgccttactt tgcgctatca gcgatttggt
     4981 cctgatttgc gcggggattt ttggtggtag cgcgttattg atgcagtcgc cgtggttgct
     5041 agcgctggtc acctggggcg gcgtagtctt cttgctctgg tatggttttg gcgcttttaa
     5101 aacagcgatg agtagtaata ttgagttagc tagtgccgaa gtcctgaagc aaggcagatg
     5161 gaaaattatc gccaccatgt tggcagtgac ctggctgaat ccgcatgttt acctggatac
     5221 ttttgttgta ctgggcagcc ttggcgggca gcttgacgtc gaaccaaaac gttggtttgc
     5281 gctcgggaca attagcgcct ctttcctgtg gttctttggt ctggctattc tcgcagcctg
     5341 gctggcaccg cgcctgcgca cggcaaaatc acagcgcatt atcaatctgg tagtgggatg
     5401 tgtcatgtgg tttatagcct tgcagctggc gagagacggt attgctcatg cacaagcctt
     5461 gttcagttag gcgctatctg atggaaaaat aaaacagagg cgctaagctt gcctccagag
     5521 gtcctgaatt ttgggcaagt caattaacac ggagagacta acgtgaagtt caaagttatc
     5581 gccctggcgg cattaatggg tattagcggg atggcagcgc aggctaacga attgccggat
     5641 ggaccgcata ttgtcacctc cggtacggca agtgtggatg cggtgccaga cattgccact
     5701 cttgcgattg aagttaacgt ggccgcgaag gatgccgcta cagccaagaa acaggcagat
     5761 gagcgcgtcg cacaatacat ttccttcctt gaactcaatc agatcgcgaa aaaagatatc
     5821 agctcagcga atttacgcac ccagccagat tatgactatc aggatggtaa aagtatcctg
     5881 aaaggctacc gagctgtgag aacggtggaa gtcacgctcc gtcagttaga caaactgaat
     5941 tccttgctgg atggcgcgct gaaggcgggt cttaacgaaa ttcgttctgt gtcgctgggc
     6001 gtggcgcagc cggatgccta taaagacaaa gcgcgtaagg cagcgattga taacgcgatt
     6061 catcaggcgc aggaactggc gaacggcttt catcgtaaac tggggccggt atatagcgtg
     6121 cgctaccatg tttccaacta tcagcccagc ccaatggtgc ggatgatgaa agccgatgcc
     6181 gcgccggtgt ctgcccagga aacttacgag caggccgcta ttcagtttga tgatcaggtc
     6241 gatgtggtct tccagttaga acctgtggat caacaacccg ctaaaacacc tgcagcacaa
     6301 taatacttgt agcccgacgt attcgtcggg ctaatgcctt tacccgatat tgcctccttt
     6361 ttctgtaatt ttctgtaaaa ccatgacgta atcacctatc tttgctcgcg gtttcatcaa
     6421 ggataggctg actgaaacgg ttaagcggac aaggatgcgg ggagggggga tggacatttt
     6481 tatttcgaaa aagatgcgca atttcatttt attagcgcaa accaataata ttgcccgggc
     6541 ggcagaaaaa atccatatga cggcttcgcc atttggtaaa agcattgccg cgctggaaga
     6601 acaaattggc tatacgctat ttacccgcaa agataataat atcagcctca ataaagccgg
     6661 tcaggaactg tatcaaaaac tgttcccggt ttatcaacga ctttctgcga ttgataatga
     6721 aatccataac tcagggcgtc gttcacgaga aattgtgatt ggcatagata atacttatcc
     6781 gacaattatt ttcgatcagt tgattagtct cggagataag tatgaaggcg tgaccgccca
     6841 gccagttgag ttcagcgaaa acggcgttat cgataatctt tttgaccgtc agttagattt
     6901 tattatctcc ccacagcatg tatctgcacg agttcaggaa ctggaaaacc tgactatcag
     6961 tgaattacca ccgctgcgat tgggctttct cgtttctcgc cgttatgaag agcgtcaaga
     7021 gcaagagctg cttcaggagc tgccctggct acaaatgcgc tttcaaaacc gggctaactt
     7081 cgaagcaatg attgatgcga atatgcgacc ttgtgggatc aatcccacca ttatttatcg
     7141 tccctacagt tttatggcca aaatcagcgc cgtggagcgc ggacattttc tgacggtgat
     7201 cccgcatttt gcctggcgac tggtgaatcc ggcaacgctg aaatattttg atgcgccgca
     7261 caggccgatg tatatgcagg aatatcttta ttcaatcaga aatcatcgtt ataccgccac
     7321 gatcttcagc atattgctga agatcgtgac gggacaaacc attaacccag catcgagccg
     7381 gttgcaatta aattacggtg taagtcgaag acgtggctaa gatcgtggtg aatatgtccg
     7441 ccgggcgcat tttccagata gcgatgcaga taatcccgat acataggatg tgcacaatta
     7501 tcaatgatag tgcgggcgcg ttgaagcggg gaaagaccgc gcagatcggc gatcccttgt
     7561 tcggtgatga tcactttgac gctgtgttcg ctgtgatcaa catggctgca cattggcacg
     7621 acggttgaga tcttgccttc tttagcaatc gacggagcca taaagatcga cagatacgcg
     7681 ttgcgttcaa aatcaccgct gccgccgatg ccgttcatca gatcgacccc ggctacgtgc
     7741 gttgaatttg catgcccgta aatatcaaac tccaggccga cgttcagagc gatgacgccc
     7801 agacgacgga tgatttccgg gttattggaa atctcctgcg gacgcaacac aatgcggctg
     7861 gcaaagtaat ccatattgtc gtaaatcttg cgcagggaat cggccgagat tgtcaggctg
     7921 gaggcgctgg ccccgctgat tttgccggtt tccagtaaat gcaccaccga ttcctgtagc
     7981 acttccgaat acatcatgaa cggaggaatt tccgggtttt cccccagacg tgccattacc
     8041 gcattattga tattgcccac gccactttgc agcggcagaa attccggcgg aatacgccca
     8101 tgcgccattt cctgcaataa gaacgtgacc acgttatcgg caatctgctg gcacatggga
     8161 ttttgcttat ccagcatatt accggcgtcg ggcaagttgg tttccacgac ggcgacaatc
     8221 tttttcggat cgatttgcac atagcgggta ccgacgcgat ccattgcatg gaagatcgac
     8281 acgctattgc gccgcggtgg cgcgccagga atcacaatat ctgccagttc tgcaacgcgc
     8341 ggatcgtgat agtgattaag ttcaatgatc actttcttcg ctcgcagcag ctaggtcggc
     8401 gcattaccga tcccgctggt taaccagact cgaccatccg gtgccagtgc cgatgcttca
     8461 atgacggcaa catcaatgtc gccgaagaaa ccgtaattga ccatttgcgc cacttcgctc
     8521 aaatgcaggt caacgaaact caccgcgccc tgattgatct ttttacgtaa actggacgat
     8581 gtttgatatg gcgcacgcca ggaaacagca tcggcgtcag aaagtacatc gtcagcggcg
     8641 gcgctgattg acgcccccgt cagcaggcga atttgatacg gctttttggc ctcatgctgt
     8701 tcgttagctc tgcgggcaat cgcggcgggt agggctttcg gcgaacccgc tggggtaaag
     8761 ccgctaaatg ccaccatgtc gttatgctgg ataatttctg ccgcttcatc ggcggtcatc
     8821 cttgtccact gagtttccat ttttactcct tcgttcgcag ggattaatga ccaacgaaat
     8881 taggtttacg tttttcgagg aaagcgttca tcccttcctg gtaatcttcg ctgtcataaa
     8941 ccgcgcggcg catcccctga atacgttcaa attcatcgga gttcatggtg tgtgcttcgc
     9001 ccagtacacg cagctcttct ttgataacgg caatggctaa cggcgctttc tcagagatgt
     9061 ggtgcgccat ttgtaaggtg aaatcttcca gttcttccac ttccacaaca tggttgagga
     9121 tgccgacagc cagcgcgcgc tgggcggtga ttggcgaagc ggtaaaaatc agctctttga
     9181 caatgtggaa gcccgcgtcg cgggtcaggt tgtgaatgcc gaccaggtta tacgggacgc
     9241 cgaggtttac aggcgtcatt gagaaggttg aggtactggc ggcgatgatc agatcggaac
     9301 tcatgatcat ttcaaatgcg ccaccccaaa cactaccttc caccatcgaa atgatcggtt
     9361 tcgggaattt ttggatcatg cgggtgattt gacgcaatgg atcatcatag gagagcggat
     9421 cgcgaccgcc agacggcagt tcgtgaatat cgtgacctgc ggagaagact ttggatccac
     9481 ttggcgcccg caaaatgata cagcgaattt ccggccggtt gagatcgctt aacgcctgca
     9541 taagatcatc aataaagact ttacttaagg cattaagttt tcgtccatag ttaaactcaa
     9601 tgaccgccac tttgttgata gtggcaacgt tcacatactg ataagacata aaaattcctt
     9661 taatcaaaat attgcgtctg gataaattca ctgagctgcc gcaggccggt gcgcggtgag
     9721 agcgtattgt ttttgaccgc taaaagcgtc tggcggtaat agcgatcgaa atcttcattc
     9781 gcgaacaggt gattcagtac ttcttcttcg gtctgcttac gcagccattc caccgattgt
     9841 tgttgccgca cttgttgtaa acgaccactg gcagttagcg cggttttgaa gtcgatgatg
     9901 gcgtgccaga tctcatcgat tccacgtttt tccagtgcgc tacaagtcag accccgtggc
     9961 tgccattcgt cgtatttacg tcgcagaata tgcagggcac tctcgtacat atgccgggca
    10021 atggcgacat tggtatggtt atcgccatcg tctttgttga taacgagcag atcagccact
    10081 tccatcagcc cttttttaat gccctgcaga tcatcgccgc caccggcaat ttgcaacgag
    10141 ataaaacagt ccaccatgcg ggcgacttcc gtttccgact gcgcgatgcc aaccgtttcg
    10201 acaatcacta cgtcataacc cgccgcttcg cataacagca ttaattcccg tgctcgctga
    10261 ctggcaccgc ccagatgacc ggaggagggt accgggcgaa taaacgccgc ctcggcacgc
    10321 gccaggtcat tcatgcgggt tttatccccg agaatgctac cgccagtgac cgggctgctg
    10381 ggatcgaccg caataacagc aacctttaat ccctcacgaa tcaacaacat gccaaaggct
    10441 tcaagaaagg tacttttccc cgcgccgggt gtgccggtaa cgcccagtcg cagggcgtta
    10501 ccacagaacg gcataatagc gtcaagcagc tgtgtactta gcgcctgatg acgtgggtga
    10561 cggctttcca ccagcgtcat ggcttgggcg agcgtggcac gctcaccctg acgtaagcgg
    10621 cgaatacttt ctgccagcgt ggcttcatta atcatgatgc tggcttatca gattcagtac
    10681 gtcgcgcaca ctgtcgagca taggtgtacc tggaccataa atcgccgcca cgccgcgctc
    10741 ttgcaggaag gcgtaatcct gcggcggaat gacgccaccc gcgaccacgc agatatcttc
    10801 gcgtccccat tttttcagcg cttcgaccag ttccgggatc agcgttttat gaccggcagc
    10861 cagtgaggat gcgcccacta cgtgaacgtc gttttctacg gctaggcggg cgatctcttc
    10921 aggtgtagag aacatcgggc ttaaatctac gtcgaaaccg agatcggaat aggcgctggc
    10981 gatcactttc gcgccgcgat cgtgtccatc ctggcccatc ttagcgatca gaatgcgcgg
    11041 gcgacgacca ttgtcggcaa ggaactgctc cgtttgcgca acaatggcat cgaactcgga
    11101 ggccgatttc tcagactgat gatagctttg cgcaatcacg ccggtaacac actggcttgg
    11161 caccagataa cggtcgaaag cgacttccag cgcatcggaa atttcaccca gggtggcgcg
    11221 aacgcgagcg gcattaacag cggcagccag caggttttcg ttatgctgtg cggcgtgagt
    11281 cagggcgttc aacgcggcgg ttacggcggc atcatcacgg gtggcgcgaa tgcgttccag
    11341 cgaagcaatt tgctcgttac gcaccatcac gttgtcgatc tcaagtacat cggtttcgtc
    11401 ttcgtgatcc agtttgtact tgttgacacc aacgatgaca cgcttgccct ggtcgatcag
    11461 cgactgttcg cgcgctgagg cctcttcgat cattcgtttt ggcagacctg cttcgatcgc
    11521 tttcgccatg ccaccggctt cgtcgatctg ttggataata gctctggctt gtttgacgat
    11581 ttgatcggtc agcgactcaa tgtaatagga tccggccagt ggatcgacgg tgtggcagag
    11641 ttctgattct tcctggatga tgatctgggt gttgcgggca atgcgtgctg agaaatcggt
    11701 aggcaaacca agcgcttcgt caaaggcgtt ggtatgcagt gactgagtac cgcccagcgt
    11761 cgcagccagt gcttcaatgg tggtgcggat aacgttgtta tacggatcct gttcagtcag
    11821 gctccagcct gaggtctggc agtgggtacg cagcgccagt gatttcggat cctgtgcgcc
    11881 aaatccactg accgcttcgc tccataaata acgtgccgca cgcaacatgg cgacgttcat
    11941 aaacagatcc atgccgatac cgaagaataa cgacaggcga ggagcgaagt catcaatttt
    12001 cagtccggca gagattgctg ctttgatgta ctcaatccca tcggcgagcg taaatgctac
    12061 ctgctgcacg cagttggcac ccgcttcccc catgtggtaa ccgctgatac tgatggtatt
    12121 aaatcgcggc atgttgccgg aacaccaggc gatgatgtcg gcgataatgc gcattgacgg
    12181 ttttggtggg taaatatagg tgttgcggca gaggtactct ttgagaatat cgttttgaat
    12241 ggtgccggtc agtttatcag gtgtaacacc ttgctcttct gcggcgacga tataaaacgc
    12301 cagtactggt agcactgcgc cattcatggt catcgaaacc gacattttat ccagcgggat
    12361 ctggtcgaac aggactttca tatcttccac ggtgtcgata gcgacgcccg ctttgccgac
    12421 gtcacccgcc acgcgtgggt tatcggagtc gtagccacgg tgggtggcaa ggtcaaacgc
    12481 aacggaaaga cctttttgcc cggcggccag gttacggcga taaaaagcgt tggactcttt
    12541 tgctgttgaa aaaccagcat actgacggat ggtccacggt tgggcggtat acatagtggc
    12601 acgcgggcca cgaacgtagg gcggcaaacc aggaagggta cctgtcacct ccagattatc
    12661 gagatcggct tcggtataca gcggcttgat ggcgatccct tccgcggttt gctgaaccag
    12721 cgagtcgaca gttttctccc gacggctcaa ttccttgttg gcaagctgtt gccactcctg
    12781 ctcgttagac attatttgct ccgtaaaacg caatcaaaat gagggtattt gcgcttacgg
    12841 tgagcttttt acgtgggttt gtcgccagca aaaaaaatgc attcagacgt cgttttttgc
    12901 cgcctgagat ttgtctattt tgtgatgtga ttcaaggaat acaaaaagaa gtgaggacaa
    12961 cgcctgatat gtgctatcag gcattatttg atggattaat cctgacgaag gactttgtga
    13021 ccataatcga gtaacgcatc agtgacttta cgcatcatgc ggctttcagg agcaaagcgg
    13081 tgccagtaga gcatccgtcg ttgaaatagc ccaggcgtta agtcaatcag ttcaccgctg
    13141 gccagctctt tctcgatttg caggtgcggg atcatacagc aggtggtgcc ctggcgagca
    13201 agttgtacga acgcttctga agaattaacg atatggcagg gtacgctgcc aggaggaaga
    13261 tcgaagtttt gctgcaaaaa ggcctggtgc atatcgtcaa gatggtcaaa cgcgaccact
    13321 ggcgctttca ataatgccga acgcgttacg ccgttaggaa aatatttttc ggcaaacggt
    13381 tttgagctga cgaacagata gtcgagcgca ccgagtttat cgacaagaca actcggcagc
    13441 gcctgatgtt gaatactcac cgcgccgacc acttcgccgc ggcgcagacg ttcctgagta
    13501 cgggtttcat cttctacctg caagttgagg cggataggcg aatcagccaa cacaggagcc
    13561 agtgccggaa gcaaccacgt cgccagactg tcggcgttga ccgccagtga aagcagcagc
    13621 ggagtcgaac cggtttgttc atcgcccagc cactcttctt ccagcaactc cacctggcgc
    13681 agcagtgcca gcagtttttg cccttgttcc gtcgggcgcg gcggtacggt acgcaccaac
    13741 agcggctgcc cgaacatatt ttccagttgc ttaatgcgct gtgagacggc tgattgtgta
    13801 atgcacagct tttgtgccgc gcgctcaaat cctcgttcac gtatcaccgc atccagtgcc
    13861 tgtaatgttc tgtagtccgg gcgtttcatt gctgttgtct gctcctggta aatgcttttt
    13921 cctgcactat gacacaattt tgtgtcaggt tgcatatacc gaagccgtcg gcgggtaaca
    13981 tttgtatcaa tggttaaacg cgcagcttaa aagatcaggg atttgcgcta ttgcatttcg
    14041 cttagcgact tcgcgccctg gttatgttga atggcgtggc gttattgcct ctgttcgcac
    14101 cgtggagcgg catacccaga ccgttgtatg ctttatttgc ggggcgaaag gggatgcccg
    14161 ccattgcgcg atattttatg gatgagttaa ccacccggct tgccaacggg gtctgaatcg
    14221 ctttttttgt atataatgcg tgtgaaattt cataccacag gcgaaacgat catgacgcag
    14281 gatgaattga aaaaagcagt aggatgggcg gcacttcagt atgttcagcc cggtaccatt
    14341 gttggtgtag gtacaggttc caccgccgca cactttattg acgcgctcgg cacgatgaaa
    14401 ggccagattg aaggggccgt ttccagttca gatgcttcca ctgaaaaact gaaaagcctc
    14461 ggcattcacg tttttgatct caacgaagtc gacagccttg gcatctacgt tgatggcgca
    14521 gatgaaatca acggccacat gcaaatgatc aaaggcggcg gcgcggcgct gacccgtgaa
    14581 aaaatcattg cttcggttgc ggaaaaattt atctgtattg ccgacgcctc caagcaggtt
    14641 gatattctgg gtaaattccc gctgccagta gaagttatcc cgatggcacg tagtgcagtg
    14701 gcgcgccagc tggtgaaact gggcggtcgt ccggaatacc gtcagggcgt ggtgaccgat
    14761 aatggcaacg tgatcctcga cgtccacggc atggaaatcc ttgacccgat agcgatggaa
    14821 aacgccataa atgcgattcc tggcgtggtg actgttggct tgtttgctaa ccgtggcgcg
    14881 gacgttgcgc tgattggcac acctgacggt gtcaaaacca ttgtgaaatg atctgacggg
    14941 ggaacctccc ccgttaaaaa aattctcttc attaaatttg gtgacatgtg tcacgctttt
    15001 accaggcaat tgtcgattgc tctaaataaa tcctctaaac cagcatattc atccaagaat
    15061 tacctttgcg tgatatttcc tcaacatcgc gacgcaaacg ttcatattgc cgcaatatta
    15121 ttttttgata tgttgaaagg cggatgcaaa tccgcacaca acatttcaaa agacaggatt
    15181 gggtaaatgg caaaggtatc gctggagaaa gacaagatta agtttctgct ggtagaaggc
    15241 gtgcaccaaa aggcgctgga aagccttcgt gcagctggtt acaccaacat cgaatttcac
    15301 aaaggcgcgc tggatgatga acaattaaaa gaatccatcc gcgatgccca cttcatcggc
    15361 ctgcgatccc gtacccatct gactgaagac gtgatcaacg ccgcagaaaa actggtcgct
    15421 attggctgtt tctgtatcgg aacaaaccag gttgatctgg atgcggcggc aaagcgcggg
    15481 atcccggtat ttaacgcacc gttctcaaat acgcgctctg ttgcggagct ggtgattggc
    15541 gaactgctgc tgctattgcg cggcgtgccg gaagccaatg ctaaagcgca ccgtggcgtg
    15601 tggaacaaac tggcggcggg ttcttttgaa gcgcgcggca aaaagctggg tatcatcggc
    15661 tacggtcata ttggtacgca attgggcatt ctggctgaat cgctgggaat gtatgtttac
    15721 ttttatgata ttgaaaacaa actgccgctg ggcaacgcca ctcaggtaca gcatctttct
    15781 gacctgctga atatgagcga tgtggtgagt ctgcatgtac cagagaatcc gtccaccaaa
    15841 aatatgatgg gcgcgaaaga gatttcgcta atgaagcccg gctcgctgct gattaatgcc
    15901 tcgcgcggta ctgtggtgga tattccggcg ctgtgtgatg cgctggcgag caaacatctg
    15961 gcgggggcgg caatcgacgt attcccgacg gaaccggcga ccaatagcga tccatttacc
    16021 tctccactgt gtgaattcga caacgtcctt ctgacgccac acattggcgg ttcgactcag
    16081 gaagcgcagg agaatatcgg cttggaagtt gcgggtaaat tgatcaagta ttctgacaat
    16141 ggctcaacgc tctctgcggt gaacttcccg gaagtctcgc tgccactgca tggtggtcgt
    16201 cgtctgatgc acatccacga aaaccgtccg ggcgtgctaa ctgcgctgaa caaaatcttc
    16261 gccgagcagg gcgtcaacat cgccgcgcaa tatctacaaa cttccgccca gatgggttat
    16321 gtagttattg atattgaagc tgacgaagac gttgccgaaa aagcgctgca ggcaatgaaa
    16381 gctattccgg gtaccattcg cgcccgtctg ctgtactaat tccccttctc tgaaaattaa
    16441 cgggcaggtc actgacttgc ccgttttttt atcccttctc cacaccgttg ccgcgttgcg
    16501 cattctactt tgcgagtctg ctccagaaaa taaatcaaag tcagatcaat gcgttgtgtt
    16561 tatttaatgg ggatttacga tggcgggaca gcatggggct gtaacggtaa agccctcacc
    16621 gaagcgaggg cttaaaggag aagggttatg atgcgacttg tcatcatact gattgtactg
    16681 ttactgataa gtttcggagc ttattaacag tcagtctcaa gggaggagtc atcctccctt
    16741 acccttactc actaaattag gtcaaagaat caacgatgtc aatcagggcg atgcggggtg
    16801 catcgccctg aaccgaactt tttgatagcc acttcatcgc ccacgtttac tgccgtaata
    16861 gatccttttt cccaaaaatt atctctgttt tgcatggggt ttttcgagca cgctcgaaga
    16921 aaagatttaa aggtagatca atgcgttgtg gttatttaaa gagagcttac gatagctggg
    16981 cagcatgggg ctgtaacggt aaagccctca ccgaagcgag ggcttgaagg agaagggtta
    17041 tgatgcgact tgccatcata ctgattgtac tgttactcat aagtttccca gcttattaac
    17101 agtcagtcac aggggaggag caatcctccc ttacccttac tcactaaatt aggttaaaga
    17161 atcaacgatg tcaatcaggg cgatgcaggt gtatcgccct taccactccc agactttcga
    17221 cggtgtaacc accgcaggaa gagggatatc ccactcttca acggggagtt tttccaccaa
    17281 ctgacaatca tgcgcataac ccaccggttg cgttttatag tgctgccagt tttgtaaggt
    17341 ccgatcataa aaaccaccgc ccattcccag acgctgaccg tactcatcaa aggcgaccag
    17401 cggtgtgatc agcacgtcta atcgggaaag gggtagcacg tcacgcacat ccagttttgg
    17461 ctcatggatc ttcaacctgt tcatcaccag ttcgctttgc ggatggtaat tcaggaacag
    17521 caaattaccg gcactaaagg gatgcaaaac tggaagatat acgcgcttac cggcgcgcca
    17581 gagttgttct attagcggct gggtgtcgag ttcaccatca aaagagagga atacagcgac
    17641 cgtatgcgcc atcaccaccg ggggataagt catcatccgg gtagcggctt gttgacccat
    17701 ttccagctgt tgttccggcg ttaacgcacg acgacgttgc cgaatcattt tgcggatttc
    17761 ttgtcgggat aatgtcagtg ggagctctgg tagttgcgtc atggctggtg ccagataaga
    17821 agggaatctc cgagatgccg ccgcaggctg taacccttga acccttggtt caaggtgaat
    17881 gtgtcgtcgc agttttaagg cttctcggac gaaccgagca tgctcaccaa ccgcggagcg
    17941 ccacattctt gtggtatgaa atatcggctc aggggactgg cccgcttgcg aacatctcag
    18001 agaaattttg tcttcacggt tactctacca cagtaaaccg aaaagtgtca ttcaaagttt
    18061 tggttagttt tttcggtgat gcgaccttgt tcaagtaacg cttgttctat ggtctgctgc
    18121 agcatccgaa tacgctgttc catacttgcc gcgtagtcac gagtctttgc tttttcttgc
    18181 gctaactcat agctgatatt caatgcggca atgaagacca actgttcagt atttgtgact
    18241 ctagtgcgtt ctttcagatc ttgcaaccgt tggttcagat cgtccgctgc ctgattcaac
    18301 gcatcccttt ggtcaggcgg gcagttcaca cgcagtgaac ggccaaaaat ttggatatcg
    18361 acgggttgtg cagacatgcc accttcctgc tgattgactg cgctgccttc gtctttagac
    18421 ccatggtctg cgaaggggcg acactatagc taccctgatg agaagagaca agcccttttc
    18481 tggtccacca gggcccaaag tggtagcata tcatgaatat tcctcccttt gacgacgaat
    18541 gcttatgtct atacagaacg aaatgcctgg ttacaacgaa atgaaccagt atctgaacca
    18601 acaagggacg ggtctgaccc ctgccgagat gcatggttta atcagtggga tgatatgtgg
    18661 cggtaacgat gacagctcat ggctgccgct acttcacgac ctgacgaacg aaggcatggc
    18721 tttcggtcat gagctggcac aggcactgcg taaaatgcac tctgccacca gcgatgccct
    18781 gcaggatgac ggcttccttt ttcagcttta tctgcctgat ggcgatgatg tcagcgtttt
    18841 cgatcgggct gatgcactgg caggttgggt caatcacttc ctgcttggtc ttggcgttac
    18901 gcaaccgaag ctggacaaag tgaccggcga aaccggtgaa gccatcgacg atctgcgtaa
    18961 catcgcgcag ctgggctacg acgaagacga agatcaggaa gagcttgaaa tgtcgcttga
    19021 agagatcatc gaatacgttc gtgttgccgc gctgttatgc cacgacacct ttactcatcc
    19081 gcaaccgacc gcgccagaag tacaaaaacc gactctacac taaaaacaaa aaacgtaagg
    19141 agagtgttat gagtgagata tcccggcaag agtttcagcg tcgccgtcag gccctggtgg
    19201 agcaaatgca acccggcagc gccgcgctga tttttgctgc accagaagta acacgtagcg
    19261 ccgacagcga atacccctat cgtcagaaca gtgacttctg gtacttcacc ggctttaacg
    19321 aaccggaagc ggtgctggtg ctgattaaaa gcgatgacac tcataaccac agcgttctgt
    19381 ttaaccgcgt tcgcgacctg acggcggaga tctggtttgg ccgtcgctta ggccaggatg
    19441 ccgcgccaga gaaactgggc gttgaccgcg cactggcatt cagcgaaatc aatcagcaac
    19501 tttatcaact acttaacggc ctggatgtgg tttaccatgc ccagggcgaa tatgcatatg
    19561 ctgatgaaat cgtgaacagt gcgctggaaa aactgcgtaa aggttcgcgg caaaatctca
    19621 ccgcaccggc aacgatgatc gactggcgtc ctgttgttca tgaaatgcgc ctgttcaaat
    19681 cgccagaaga gattgccgta ctccgccgcg cgggagaaat caccgccatg gcacatacac
    19741 gggcgatgga aaaatgccgt ccgggaatgt tcgagtacca tctggaaggc gaaattcacc
    19801 acgaatttaa ccgccacggt gcgcgctatc cttcctataa caccattgta ggcagcggtg
    19861 aaaacggctg cattctgcac tacaccgaaa acgagagtga actgcgcgac ggtgacctgg
    19921 tgttgattga cgcgggttgt gaatacaaag gttacgcggg cgatattacc cgcaccttcc
    19981 cggtcaacgg caaattcacc caggcccagc gtgaaatcta cgacattgtg ctggagtctc
    20041 tcgaaaccag cctgcgcctg tatcgtccgg gaacttccat tctggaagtc actggtgaag
    20101 tggtgcgcat catggttagc ggcctggtaa aactcggcat cctgaaaggt aatgttgatg
    20161 aactgatcgc tcagaacgcc catcgtcctt tctttatgca tggccttagc cactggttag
    20221 gactggatgt ccatgacgtt ggcgtttatg gtcaggatcg ctcgcgcatt ctggaaccgg
    20281 ggatggtact gaccgtagag cctggtctgt atatcgcgcc ggatgcggat gtgccagaac
    20341 aatatcgcgg tatcggcatt cgtattgaag acgacattgt gattaccgaa accggcaacg
    20401 aaaacctcac cgccagcgtg gtgaaaaagc cggaagaaat cgaagcgttg atggctgctg
    20461 cgagaaagca atgagcgtaa tcatcgtcgg tggcggcatg gcgggcgcga cgctggcgct
    20521 ggctatttcc cggttaagtc acggggcgct gccggtacat ttgattgaag cgactgcgcc
    20581 agagtcacat gctcatccgg gctttgatgg acgagcgata gcgctggcgg cgggtacctg
    20641 tcagcaactg gcgcgcatcg gcgtctggca atctctggct gattgcgcaa ccgccatcac
    20701 taccgtgcat gtcagcgatc gtggtcatgc tggattcgtc accctcgccg cagaagatta
    20761 ccaactggcg gcgctggggc aggttgtcga attgcataat gtcgggcaac ggctgtttgc
    20821 attgctgcga aaagcacctg gcgtaatgct gcattgcccc gatcgcgtag ctaacgttgc
    20881 ccgtactcag agtcatgttg aagtgacgct ggagagcggc gagatgctga cgggccgcgt
    20941 gctgatagcc gctgatggca cccattcagc gttagccacc gcgtgcggcg ttgattggca
    21001 gcaggagcct tacgaacaac tggcagtgat tgccaacgtt gctacttctg ttgcgcacga
    21061 aggccgcgct tttgaacgct tcacgcaaca tggcccgctg gcgatgttgc cgatgtctga
    21121 cggacgctgt tcgctggtct ggtgtcatcc actggaacgg cgcgaagagg tgctgtcgtg
    21181 gagcgacgag aagttttgcc gtgaactcca gtctgccttt ggctggcgac tggggcaaat
    21241 tacccacgcc ggtaaacgca gtgcttatcc gctggcatta acccgcgccg ccagaccgat
    21301 tacccatcgc actgtgctgg tgggcaatgc ggcgcaaact ctgcacccga ttgccgggca
    21361 agggtttaac ctcggtatgc gagatgtcat gagccttgcg gaaaccctga ctcaggcgca
    21421 tgagcgcgga gaagacatcg gtgattacgg catattgtgc cgctatcagc agcgtcgaca
    21481 gagcgatcgc gaagcaacca tcggcgtcac ggacagcctg gtacatcttt ttgccaaccg
    21541 ttgggcaccg ctggttgtcg ggcgcaacat cggactgatg acgatggaat tattcacccc
    21601 ggcacgcgat gtgctggcgc agcgcaccct cggttgggtg gcgcgttgaa ggcggtttta
    21661 aggaataaac aatgcaaagt gttgatgtag ccattgttgg cggcggcatg gtggggctgg
    21721 cggttgcctg cggcttacag gggagcggct tacgcgttgc cgtactggag cagcgcgtac
    21781 cggaacctct ggcggtagat gcactaccac aactgcgcgt ttcggctatc aatgccgcca
    21841 gcgaaaaatt actcacccgt cttggcgtct ggcaggacat tctcgctcgt agggccagtt
    21901 gttatcacgg catggaagtg tgggacaaag acagttttgg tcacatctcg tttgacgatc
    21961 aaagcatggg ctatagccat cttgggcaca tcgttgaaaa ctcagtgatt cactacgcgc
    22021 tgtggaacaa agcgcagcag tcgtcagata ttactctgtt ggcccccaca gaattacagc
    22081 aggtcgcctg gggagaaaat gaaaccttcc tgacgctgaa agatggcagc atgttaacgg
    22141 cgcgtctggt gattggcgcg gacggtgcta attcctggtt gcgcaacaaa gcagatattc
    22201 cgctgacttt ctgggattat cagcatcacg cgctggtagc gaccattcgc acggaagaac
    22261 cgcatgatgc ggtggcgcgg caggttttcc atggcgaagg cattctggcc tttttaccgc
    22321 ttagcgatcc gcatctttgc tcgattgtct ggtcactgtc gccagaggaa gcgcagcgga
    22381 tgcagcaggc aagtgaagac gaatttaatc gcgcgttaaa tatcgctttt gataatcgcc
    22441 ttggcttatg caaggttgag agcgcgcgtc aggtgttccc gctgacgggg cgttatgcgc
    22501 gccagtttgc cgcacaccgt ctggcgctgg tgggcgacgc cgcgcatacc attcacccac
    22561 tggcggggca gggcgttaat ctcggattta tggacgccgc agagctgatt gctgaactga
    22621 aacggttgca tcgtcagggt aaagacatcg ggcagtacat ttacctgcgc cgctatgaac
    22681 gtagccgcaa gcacagcgcg gcgctgatgc tggctggtat gcagggtttt cgcgatctct
    22741 tttccggcgc taatccggcg aaaaaactgc tgcgtgatat tggtttgaaa ctggccgaca
    22801 cgcttcctgg cgttaagccg caacttatcc gccaggcaat gggattaaac gatttgcctg
    22861 aatggctgcg ttaaaaattt ctcctctgtt gtttatttga tacccatcac actttcatct
    22921 cccggttttt tcgccgggag attttcctca tttgaaataa actaatttca cctccgtttt
    22981 cgcattatat tttctaatac catgattttt tgatttagtg ttttttgaca tttttttagc
    23041 tcttaatatt gtcttattca aattgacttt ctcatcacat catctttgta tagaaattgg
    23101 tgtatttttt ggttttttat tctgtcgcga tttttgcatt ttttaaccat aagctaatgt
    23161 gatgatcaat tttaccttat ggttaacagt ctgtttcggt ggtaagttca ggcaaaagag
    23221 aacgattgcg ttggggaccg ggagtggctc cgatgctggg ttttgtggtg ataatttcac
    23281 catgaaaaag ttgtcagccc cgcttattca atgaggacaa gatggcacaa cagactcctt
    23341 tgtacgaaca acacacgctt tgcggcgctc gcatggtgga tttccacggc tggatgatgc
    23401 cgctgcatta cggttcgcaa atcgacgaac atcatgcggt acgtaccgat gccggaatgt
    23461 ttgatgtgtc gcatatgacc atcgtcgatc tccgcggcag ccgcacccgg gagtttctgc
    23521 gttatctgct ggcgaacgat gtggcgaagc tcaccaaaag cggcaaagcc ctttactcgg
    23581 ggatgttgaa tgcctctggc ggtgtgatag atgatctcat cgtctactac tttactgaag
    23641 atttcttccg cctcgttgtt aactccgcca cccgcgaaaa agacctctcc tggattaccc
    23701 aacacgctga acctttcggc atcgaaatta ccgttcgtga tgacctttcc atgattgccg
    23761 tgcaagggcc gaatgctcag gcaaaagctg ccacactgtt taatgatgct cagcgtcagg
    23821 cggtggaagg gatgaaaccg ttctttggcg tgcaggtggg cgatctgttt attgccacca
    23881 ctggttatac cggtgaagcg ggctatgaaa ttgcgctgcc caatgaaaaa gcggccgatt
    23941 tctggcgtgc gctggtggaa gcgggcgtta agccctgcgg tctgggggcg cgtgacacgc
    24001 tgcgtctgga agcgggcatg aatctttatg gtcaggagat ggacgaaact atttctcctt
    24061 tagccgccaa catgggctgg actatcgcct gggaaccgac agatcgtgac tttatcggtc
    24121 gtgaagccct ggaagtgcag cgtgagcatg gtacagaaaa actggttggt ctggtgatga
    24181 ccgaaaaagg cgtgctgcgt aatgaactgc cggtacgctt taccgatgcg cagggcaacc
    24241 agcatgaagg cattatcacc agcggtactt tctccccgac gctgggttac agcattgcgc
    24301 tggcgcgcgt gccggaaggt attggcgaaa cggcgattgt gcaaattcgc aaccgtgaaa
    24361 tgccggttaa agtgacaaaa cctgtttttg tgcgtaacgg caaagccgtc gcgtgattta
    24421 cttttttgga gattgattga tgagcaacgt accagcagaa ctgaaataca gcaaagaaca
    24481 cgaatggctg cgtaaagaag ccgacggcac ttacaccgtt ggtattaccg aacatgctca
    24541 ggagctgtta ggcgatatgg tgtttgttga cctgccggaa gtgggcgcaa cggttagcgc
    24601 gggcgatgac tgcgcggttg ccgaatcggt aaaagcggcg tcagacattt atgcgccagt
    24661 aagcggtgaa atcgtggcgg taaacgacgc actgagcgat tccccggaac tggtgaacag
    24721 cgaaccgtat gcaggcggct ggatctttaa aatcaaagcc agcgatgaaa gcgaactgga
    24781 atcactgctg gatgcgaccg catacgaagc attgttagaa gatgagtaac ggctttattc
    24841 ctcttctgcg ggagaggatc agggtgagga aaatttatgc ctcaccctca ctctcttcgt
    24901 aaggagagag gttcacaatt cactgcacgt ttcaggaacc atcgctcatg acacagacgt
    24961 taagccagct tgaaaacagc ggcgctttta ttgaacgcca tatcggaccg gacgccgcgc
    25021 aacagcaaga aatgctgaat gccgttggtg cacaatcgtt aaacgcgctg accggccaga
    25081 ttgtgccgaa agatattcag cttgcgacac caccgcaggt tggcgcaccg gcgaccgaat
    25141 acgccgcact ggcagaactc aaggctattg ccagtcgcaa taaacgcttc acgtcttaca
    25201 tcggcatggg ttacaccgcc gtgcagctac cgccggttat cctgcgtaac atgctggaaa
    25261 atccgggctg gtataccgcg tacactccgt atcagcctga agtctcccag ggccgccttg
    25321 aagcactgct caacttccag caggtaacgc tggatttgac tggactggat atggcctctg
    25381 cttctcttct ggacgaggcc accgctgccg ccgaagcaat ggcgatggcg aaacgcgtca
    25441 gcaaactgaa aaatgccaac cgcttcttcg tggcttccaa tgtgcatccg caaacgctgg
    25501 atgtggtccg tactcgtgct gaaacctttg gttttgaagt gattgtcgat gacgcgcaaa
    25561 aagtgctcga ccatcaggac gtcttcggcg tgctgttaca gcaggtaggc actaccggtg
    25621 aaattcacga ctacactgcg cttattagcg aactgaaatc acgcaaaatt gtggtcagcg
    25681 ttgccgccga tattatggcg ctggtgctgt taactgcgcc gggtaaacag ggcgcggata
    25741 ttgtttttgg ttcggcgcaa cgcttcggcg tgccgatggg ctacggtggc ccacacgcgg
    25801 cattctttgc ggcgaaagat gaatacaaac gctcaatgcc gggccgtatt atcggtgtat
    25861 cgaaagatgc agctggcaat accgcgctgc gcatggcgat gcagactcgc gagcaacata
    25921 ttcgtcgtga gaaagcgaac tccaacattt gtacttccca ggtactgctg gcaaacatcg
    25981 ccagcctgta tgccgtttat cacggcccgg ttggcctgaa acgtatcgct aaccgcattc
    26041 accgtctgac cgatattctg gcggcgggcc tgcaacaaaa aggtctgaaa ctgcgccatg
    26101 cgcactattt cgacaccttg tgtgtggaag tggccgacaa agcgggcgta ctgacgcgtg
    26161 ccgaagcggc tgaaatcaac ctgcgtagcg atatcctgaa cgccgtgggg atcacccttg
    26221 atgaaaccac cacgcgtgaa aacgtaatgc agcttttcag tgtgctgctg ggcgataacc
    26281 acgggctgga gatcgacacg ctggacaaag acgtggctca cgacagccgc tctatccagc
    26341 ctgcgatgct gcgcgacgac gaaatcctca cccatccggt gtttaatcgc tatcacagcg
    26401 aaaccgaaat gatgcgctat atgcactcgc tggagcgtaa agatctggcg ctgaatcagg
    26461 cgatgatccc gctgggttcc tgcaccatga aactgaacgc cgccgccgag atgatcccaa
    26521 tcacttggac ggaatttgcc gaactgcacc cgttttgccc gccggaacag gctgaaggtt
    26581 atcagcagat gattgcgcag ctggctgact ggctggtgaa actgaccggt tacgacgccg
    26641 tttgtatgca gccgaactcc ggcgcacagg gcgaatacgc gggcctgctg gcgattcgtc
    26701 attatcatga aagccgcaac gaagggcatc gcgatatctg cctgatcccg gcttctgcgc
    26761 acggaactaa ccccgcttct gcacatatgg caggaatgca ggtggtggtt gtggcgtgtg
    26821 ataaaaacgg caacatcgat ctgactgatc tgcgcgcgaa agcggaacag gcgggcgata
    26881 acctctcctg tatcatggtg acttatcctt ctacccacgg cgtgtatgaa gaaacgatcc
    26941 gtgaagtgtg tgaagtcgtg catcagttcg gcggtcaggt ttaccttgat ggcgcgaaca
    27001 tgaacgccca ggttggcatc acctcgccgg gctttattgg cgcggacgtt tcgcacctca
    27061 acctgcataa aactttctgc attccgcacg gcggtggtgg tccgggtatg ggaccgatcg
    27121 gtgtgaaagc gcatctggca ccgtttgtac cgggtcatag cgtggtgcaa atcgaaggca
    27181 tgttaacccg tcagggcgca gtttctgcgg caccgttcgg tagcgcctct atcctgccaa
    27241 tcagctggat gtacatccgc atgatgggcg cagaagggct gaaaaaagca agccaggtgg
    27301 caatcctcaa cgccaactat attgccagcc gcctgcagga tgccttcccg gtgctgtata
    27361 ccggtcgcga cggtcgcgtg gcgcacgaat gtattctcga tattcgcccg ctgaaagaag
    27421 aaaccggcat cagcgagctg gacattgcca agcgcctgat cgactacggt ttccacgcgc
    27481 cgacgatgtc gttcccggtg gcgggtacgc tgatggttga accgactgaa tctgaaagca
    27541 aagtggaact ggatcgcttt atcgacgcga tgctggctat ccgcgcagaa attgaccagg
    27601 tgaaagccgg tgtctggccg ctggaagata acccgctggt gaacgcgccg cacattcaga
    27661 gcgaactggt cgccgagtgg gcgcatccgt acagccgtga agttgcggta ttcccggcag
    27721 gcgtggcaga caaatactgg ccgacggtga aacgtctgga tgatgtttac ggcgaccgta
    27781 acctgttctg ctcctgcgta ccgattagcg aataccagta attcactgat tcgagtattt
    27841 tctaaaggcg cttcggcgcc tttttagtca gatgacaaag tacaaaagtg ctcagacagt
    27901 cccctcgccc ctttggggag agggttaggg tgaggggaac aggccggcac tggcgcgaat
    27961 atttaccctc accccgaccc tctccctgaa agggcgaggg ggaaaagcgt gccaacattg
    28021 aagattgagc cagtttgtta gcaatctcaa agatacgtca acgaattaat ttttctcgga
    28081 aaaacaaatg gctatagcac ttgtgaccgg tggcagtcgc ggcatcgggc gggcaactgc
    28141 attactgttg gcgcaagaag ggtatacggt ggcggttaat tatcagcaaa acctccacgc
    28201 ggcgcaggaa gtgatgaact taataatgca agccggtggc aaagcattcg tgctccaggc
    28261 ggatatcagc gacgaaaacc aggtcgttgc gatgtttaca gcaatcgatc agcacgatga
    28321 accgctagca gcgctggtca ataacgccgg gatcttgttt acccagtgca ccgttgaaaa
    28381 ccttaccgca gagcgaatca accgagtact ttccaccaac gtgacgggat attttctctg
    28441 ctgccgcgag gcggtaaaac gcatggcgct taaaaatggt ggcagtggcg gcgctatcgt
    28501 caatgtctct tcggtggcct cacggttggg ttcgccaggg gaatatgttg attacgcggc
    28561 atcgaaaggg gcgattgatt cgttaaccac cggactatcg ctggaagtcg ccgcgcaggg
    28621 gatccgcgtt aactgcgtgc ggccagggtt tatttatacc gaaatgcacg ccagcggcgg
    28681 cgagcctgga cgcgtcgatc gcgttaagtc gaacatcccc atgcagcgtg gtggacaggc
    28741 agaagaggtc gcgcaggcca ttgtctggct actaagtgat aaagcctctt acgtcacggg
    28801 aagttttatc gatttggcgg gcgggaaata aaacagggaa gttgtctgac cggatgcaac
    28861 aagtattgca tccggtactt catcgactta aagcttctcg ccgttgctgg caatcacctc
    28921 tttgtaccag ttaaagctct tcttacgtga acgcgacata tcgccagtac cgtcgtcatg
    28981 tttattcaca tagataaagc cgtagcgttt gctgtactgc ccggtagtga atgacacgca
    29041 gtcgatgcag ccccacggtg tgtagcccat cagatccaca ccatcgtaag tcactgcttt
    29101 tttcatctct tcaatatggg cgcgcaggta gtcaatgcgg tagtcgtcgt tgatgctgcc
    29161 atcttcttcc actttgtcgt aagcgccaaa accgttttcg acaataaaca gcggcctctg
    29221 ataacgctca tacagttcgc aaagggcata gcgcagacct actggatcaa tctgccagcc
    29281 ccagtcagac gctttaacat acgggttcgg tacgctgcct tcaaaaccag agatcgcatc
    29341 gccggtgccg ccttcggcct tcactgcgtt ggtcatgtaa tagctgaaac caagataatc
    29401 gcaggtgcct tcgcgcagta catcgagatc gccgtcttcc attttgatat taaatccgcg
    29461 acgttcccac tcgttcaata cataggacgg gtaatagccg catagctgca catcggtaaa
    29521 gacgtagcgt tcgcgcatcg actcctgagc gaacatcaca tcgtccgggt tacatgagta
    29581 gggatagagc ggcaccatcg ccagcataca gccgactttc atctccgggt taatgcgacg
    29641 cgcggctttc actgccaggg cgctggcgac aaactggtga tgcagcacct ggtacatcgt
    29701 ctcttccggg ttgtcatgct cggtatacac cacgccggag cagcagtaac caaacagcgg
    29761 cgcacgccag ttacgctggt tgtttatttc gttgaaggtc atccagtatt tgactttatg
    29821 cttgtagcgt tcgaatacca cttccgcgaa acggacaaag aaatcaacga atttacggtt
    29881 ggtccagcta ccgtattgct gcaccagatg cagcggcatt tcaaagtggg agagggttat
    29941 caccggttcg atgttgtatt tcagcagttc atcgaacaga gagtcgtaaa acttcagccc
    30001 ttcttcgttt ggctgagctt catcaccttt tggaaaaata cgcgtccagg cgatggaagt
    30061 ccgaaaacat ttgaagccca tttcggcaaa tagcttgatg tcttccttgt agtgaccata
    30121 aaaatcaacg gcttcatggt ttggatagta ttttcctggc aagacttctt tggtgatttc
    30181 gcgcggcacg ccgtgtgcgc cacaggtcag aacgtcacaa atgctcggtc ctttgccgcc
    30241 tttgttccag ccgccttcga cctgatgagc ggcaacggcg ccaccccata agaaatcttt
    30301 cggtaaggtg agttttttca ctatcatagg ctccagatca gtcaattctt acaggaaata
    30361 gtaccatgca gccaaacgac atcacttttt tcaacgtttc caggatgaca ttctggctgg
    30421 gcgtaaaacc atcaccatcc gcgacgagtc tgaatcgcac ttcaaaacgg gtgatgtgct
    30481 tcgtgtcgga cgttttgaag atgacggtta tttttgcacg attgaagtca ccgcaacctc
    30541 aaccgtaacg ctggatacac tgacggaaaa acatgcagag caggaaaata tgaccctgcc
    30601 tgaactgaaa aaggtcattg ctgacatcta tcccggtcag acacaatttt atatgattga
    30661 atttaaatgt ctttaaattg aactcacgcc tgttagttga aatttcgcat ttaatttcat
    30721 gatttttagg agattagtct caaatcagaa tttattttag ctaacaggtg ttcactggaa
    30781 ctattctcag ttacgctgga gaggtataca tgtgcgtgta cgtttctctg gagtaagtta
    30841 tggttcagaa gcccctcatt aagcagggat attcgctggc agaggaaatt gccaacagcg
    30901 tcagtcacgg cattgggctg gtgtttggta tcgttgggct ggtgttgcta ctggttcagg
    30961 cggtggatct caatgccagc gccacagcga taaccagtta cagcctctat ggcggcagta
    31021 tgatcctgct gttcctcgct tcgacgctct atcacgccat tcctcatcaa cgggcaaaaa
    31081 tgtggctgaa gaaatttgac cattgcgcta tttacctgtt gattgccgga acctacacgc
    31141 cgtttttgct ggtggggctg gattctccgt tagcgcgcgg gttgatgatt gttatctgga
    31201 gcctagcatt gctggggatt ctgttcaaac tgaccatcgc gcaccgattc aaaattttat
    31261 ctctggtgac ttatctggcg atgggctggc tgtcgctggt ggtaatttat gaaatggcag
    31321 ttaagctcgc ggcgggcagc gttaccttac tggcggtagg cggcgtggtt tattcgctcg
    31381 gggtgatttt ctacgtctgc aaacgcattc catacaacca tgccatctgg cacggcttcg
    31441 tgctcggcgg tagtgtgtgc cactttctgg cgatctattt gtatattggg caggcgtaat
    31501 gggtatgcct ttgtaggcct gataagacgc gtcagcgtcg catcaggcat cggagcgcaa
    31561 ccgccggatg cggcgtgaac gccttatccg atctacgggc ctcgtccctg ttgtgataaa
    31621 ggcatcaaat cccagacatg ataagacgcg tcagcgtcgc atcaggcgat aaatgcctga
    31681 tgcgatacag agatttactc ttcgagcgaa tacggcagcg gctcgatatg cagtgtattc
    31741 gcatcgtcgc gtacgcggaa gatgctgtcc ggttccatat cgttattcat gacgacctgt
    31801 accacgacct gaccatcttc cagttttacc gcagccagca ccgtaccggt acgacgccag
    31861 ttctcgccca ttttcagctc taagtcttca ccagcttccg gcagtcggct ggcactacct
    31921 gccagcaacc agagcgcgcg tttgttggca ccacggaatt ttgctcgcgc caccatctct
    31981 tgtccggtat aacagccttt cttaaagctg ataccgccca gcgcctggag attggtcgcc
    32041 tgtgggataa actgcccgct gttggcggca tcaatcaccg ggaaaccagc ttcaatgttt
    32101 aatgccagcc actgttggct attgttcagt tccgcttcac cgcgcagttt atcggtcagc
    32161 atattagcag tagcttcatc ggttacgatc aggaaacgtt ctgccgggtg ttcaaaccat
    32221 agcaaagtgg tcgcgccttc tttgactacc tgtttttctt tcgaaggcag ttcgctaaag
    32281 agatttgcca gcgcggcgcg cgcctgaaaa ccggcaacac caagcagcac acgctcgtcg
    32341 tctggcgcga tggtcacttt agagaatacc gcatattttt tcagttcagt cagctgcggt
    32401 tcacgcacgc tgcgccgttc aatccatgca aagccatcgc cgtcgcggaa cagacgtaaa
    32461 ttgctccaca ttttaccttt ggcgtcgcaa tgggcggcga gcaggtgctg atcttctgcc
    32521 atctggctga catctgctgt cacctgaccc tgcatatatt tttcgctgtc cgcgccagta
    32581 atggtggcaa gcgcccagtc atcaagcgtc atcagcgtca gcggtaaacg ggcagaagcc
    32641 gtaggctgac gtggaggaaa aggtgtaaaa gccataatag agtcctgagt tgcttaacgg
    32701 cgtattaatg actaatggta aaagagctat gaggcaatgc aagtgctttc actacgcgat
    32761 ctgtgccttc tgcgaggcga ctgccaaaaa aatgaagccc ttgaattgtt gtgaattaat
    32821 ttcgcgatcg cgatggtgtt tactgatatt cccgtaaaag aagtcgaaaa acacgttaca
    32881 atagccgggt actggatttt cgtgagacag gaagaagatg gacattaaca acaaagcccg
    32941 aattcattgg gcatgccgcc gtggtatgcg cgaactcgat atttcaatca tgtcgttttt
    33001 cgaacatgag tacgacagct taagcgatga cgaaaaacgc atctttattc gtctgctgga
    33061 atgtgacgat ccggacctgt ttaactggct gatgaatcac ggtaaaccgg ccgatgcaga
    33121 actggaaata atggtccgac tcatccagac acggaaccgg gaacgtggtc ctgtggcaat
    33181 ctgatttgcg cgtctcctgg cgcgcacagt ggctttcctt gctgattcat gggctggttg
    33241 ccgctgttat tttactcatg ccctggccgc tcagttacac cccgttatgg atggtgttac
    33301 tttcgctggt ggtatttgat tgcgttcgca gccagcggtg tattaatgct cgccaggggg
    33361 aaattcgctt gttgatggac gggcgtttgc gttggcaagg gcaggagtgg agcatcgtca
    33421 aagcaccgtg gatgattaag agcggcatga tgctgcgttt acgttctgat ggcggtaaac
    33481 ggcaacattt atggctggca gccgacagca tggacgaagc cgaatggcgg gatttacggc
    33541 ggattttgtt gcaacaagag acgcaaagat aaagatgacg gcaaagcata tgcgctaagc
    33601 cgtcagtgag atcaggcgta atgctctgcc atttcgttga ggatttgctc gcaccagctc
    33661 tgaatacgct cgtcgctaag gtcatactgg ttagtttcat ccagcgccag acccacgaac
    33721 agttgcccgt cagcaatcac cggtttcggg ctggtaaatt catatccttc cgttggccag
    33781 tagccgacga acttcacgcc tttggtcgag agtttgtcat gcagcatacc gagcgcatcg
    33841 aggaaccact cgccgtatcc cagttgatcg ccaagcccat acagcgcaat aattttacct
    33901 tcaaggttca ggtcgtcgag ctgatcccag acggcttccc agtcttcctg gatttcacca
    33961 aaatcccagg tcgggatacc cagaatgagc acatcgtact gctccattaa tttcggggag
    34021 tcgtccttga ggttatgtaa ggtcaccagt tctgggccga taatatcgcg gattttttct
    34081 gccgccattt cggtgtaaca ggtgctggaa ccgtaaaaaa gacccatatt cataacgtaa
    34141 gcttctcgat tctgctgtgg cttttgcagc tagtgtatca gaatcgcttt agtcacggca
    34201 caataaggca taatggatca aatgagtgac cataaggggc gcaagtgaaa caggagctgg
    34261 cacgcatcga gcagtttctt gatgctctgt ggctggaaaa aaatctggct gaaaatacgt
    34321 tgaacgctta ccgtcgcgat ctgtcaatga tggtggagtg gttgcatcac cgcgggttga
    34381 cgctggcgac ggcgcaaagt gacgatttgc aggcattact ggcagaacgg ctggagggcg
    34441 ggtataaagc caccagctca gcgcgtttgc tgagtgcagt gcgccgattg ttccagtatc
    34501 tttatcgcga aaagtttcgt gaagacgatc ccagtgcgca tctcgcttca ccgaaattgc
    34561 cccagcgttt gccaaaagat ttaagcgaag cgcaggtcga acgtttatta caggcgccat
    34621 taattgatca gccactggag ctacgcgata aagccatgct tgaagtgttg tatgctaccg
    34681 ggctgcgtgt ctctgaactg gtcgggctga caatgagtga tatcagcctg cgtcagggcg
    34741 tagtgcgggt cattggtaaa ggcaacaaag agcgcctggt gccgttaggg gaagaggcgg
    34801 tttactggct ggaaacctat ctggaacatg ggcgtccgtg gctgctgaat ggtgtgtcaa
    34861 ttgacgtgtt gtttcccagc cagcgtgcgc agcagatgac gcgacagacc ttctggcacc
    34921 gcattaaaca ttatgccgtg ctggcgggta tcgacagcga aaagctgtca ccgcatgtgt
    34981 tgcgtcacgc ttttgccact catttattaa atcatggtgc ggatttacgc gtggtgcaga
    35041 tgctactggg ccacagcgat ctttccacca cgcaaattta tacgcatgtc gctaccgagc
    35101 gtctgcggca acttcatcaa cagcatcacc cgcgggcgtg atgtctgaaa agaacgggaa
    35161 gatttatgaa gaaaggtttt atgttgttta ctttgttagc ggcgttttca ggctttgctc
    35221 aggctgatga cgcggcaatt caacaaacgt tagccaaaat gggcatcaaa agcagcgata
    35281 ttcagcccgc gcccgtagct ggcatgaaga cagttctgac taacagcggt gtgttgtaca
    35341 tcaccgatga tggtaaacat atcattcagg ggccaatgta tgacgttagt ggcacggctc
    35401 cggtcaatgt caccaataag atgctgttaa agcagttgaa tgcgcttgaa aaagagatga
    35461 tcgtttataa agcgccgcag gaaaaacacg tcatcaccgt gtttactgat attacctgtg
    35521 gttactgcca caaactgcat gagcaaatgg cagactataa cgcgctgggg atcaccgtgc
    35581 gttatcttgc tttcccgcgc caggggctgg acagcgatgc agagaaagaa atgaaagcta
    35641 tctggtgtgc gaaagataaa aacaaagcgt ttgatgatgt gatggcaggt aaaagcgtcg
    35701 caccagccag ctgcgacgtg gatattgccg accattacgc acttggcgtc cagcttggcg
    35761 ttagcggtac tccggcagtt gtgctgagca atggcacact tgttccgggt taccagccgc
    35821 cgaaagacat gaaagaattc ctcgacgaac accaaaaaat gaccagcggt aaataattcg
    35881 cgtgaaacaa cagatacaac ttcgtcgccg tgaagtcgat gaaacggcag acttgcccgc
    35941 tgaattgcct cccttgctgc gccgtttata cgccagccgg ggagtacgca gtgcgcaaga
    36001 actggaacgc agtgttaaag gtatgttgcc ctggcagcaa ctaagcggtg tcgaaaaggc
    36061 cgttgagatc ctttacaacg cttttcgcga aggaacgcgg attattgtgg tcggtgattt
    36121 cgacgccgac ggcgcgacca gcacggctct aagcgtgctg gcgatgcgct cgcttggttg
    36181 cagcaatatc gactacctgg taccaaaccg tttcgaagac ggttacggct taagcccgga
    36241 agtggtcgat caggcccatg cccgtggcgc gcagttaatt gtcacggtgg ataacggtat
    36301 ttcctcccat gcgggagttg agcacgctcg ctcgttgggc atcccggtta ttgttaccga
    36361 tcaccatttg ccgggcgaca cattacccgc agcggaagcg atcattaacc ctaacttgcg
    36421 cgactgtaat ttcccgtcga aatcactggc aggcgtgggt gtggcgtttt atctgatgct
    36481 ggcgctgcgc acctttttgc gcgatcaggg ctggtttgat gagcgcggca tcgcaattcc
    36541 taacctggca gaactgctgg atctggtagc actggggaca gtagcggacg tcgtgccgct
    36601 ggacgctaat aatcgcattc tgacctggca ggggatgagt cgcatccgtg ccggaaagtg
    36661 ccgtccaggg attaaagcgc tgctggaagt ggcaaatcgc gatgcacaaa aactagccgc
    36721 cagcgattta ggttttgcgc tgggaccgcg tctcaatgct gccgggcgac tggatgatat
    36781 gtccgttggt gtggcgctgt tgctgtgcga caacatcggc gaagcgcgcg tgctggcaaa
    36841 tgaactcgat gcgctaaacc agacgcgaaa agagatcgaa caaggaatgc aggttgaagc
    36901 cctgaccctg tgcgagaaac tggagcgcag ccgtgacacg ctacccggcg ggctggcaat
    36961 gtatcacccc gaatggcatc agggcgttgt cggtattctg gcttcgcgta tcaaagagcg
    37021 ttttcaccgt ccggttatcg cctttgcgcc agcaggtgac ggtacgctga aaggttccgg
    37081 tcgctccatt caggggctgc atatgcgtga tgcgctggag cgattagaca cactctaccc
    37141 cggcatgatg ctcaagtttg gcggtcatgc gatggcggcg ggtttgtcgc tggaagagga
    37201 taaattcgaa ctctttcaac aacggtttgg cgaactggtt actgagtggc tggccccttc
    37261 gctattgcaa ggcgaagtgg tgtcagacgg tccgttaagc ccggccgaaa tgaccatgga
    37321 agtggcgcag ctgctgcgcg atgctggccc gtgggggcag atgttcccgg agccgctgtt
    37381 tgacggtcat ttccgtctgc tgcaacagcg gctggtgggc gaacgtcatt tgaaggtgat
    37441 ggtcgaaccg gtcggcggcg gtccactgct ggatggtatt gcttttaatg tcgataccgc
    37501 tctctggccg gataacggcg tgcgcgaagt gcaactggct tataagctcg atatcaacga
    37561 gtttcgcggc aaccgcagcc tgcaaattat catcgacaat atctggccaa tttagcgtca
    37621 tcttctctat aaaaaagagc gtggattggg tacaatcccg ctcttatcac cgcattttga
    37681 ctagctcaat aaaagaaatc agaccatgtt tgaaattaat ccggtaaata atcgcattca
    37741 ggacctcacg gaacgctccg acgttcttag ggggtatctt tgactacgac gccaagaaag
    37801 agcgtctgga agaagtaaac gccgagctgg aacagccgga tgtctggaac gaacccgaac
    37861 gcgcacaggc gctgggtaaa gagcgttcct ccctcgaagc cgttgtcgac accctcgacc
    37921 aaatgaaaca ggggctggaa gatgtttctg gtctgctgga actggctgta gaagctgacg
    37981 acgaagaaac ctttaacgaa gccgttgctg aactcgacgc cctggaagaa aaactggcgc
    38041 agcttgagtt ccgccgtatg ttctctggcg aatatgacag cgccgactgt tacctcgata
    38101 ttcaggcggg gtctggcggt acggaagcac aggactgggc gagcatgctt gagcgtatgt
    38161 atctgcgctg ggcagaatcg cgtggtttca aaactgaaat catcgaagag tcggaaggtg
    38221 aagtggcggg tattaaatcc gtgacgatca aaatctccgg cgattacgct tacggctggc
    38281 tgcgtacaga aactggcgtt caccgcctgg tgcgtaaaag cccgtttgac tccggcggtc
    38341 gtcgccacac gtcgttcagc tctgcgtttg tttatccgga agttgatgat gatattgata
    38401 tcgaaatcaa cccggcggat ctgcgcattg acgtttatcg cgcgtccggc gcgggcggtc
    38461 agcacgttaa ccgtaccgaa tctgcggtgc gtattaccca catcccgacc gggatcgtga
    38521 cccagtgcca gaacgaccgt tcccagcaca agaacaaaga tcaggccatg aagcagatga
    38581 aagcgaagct ttatgaactg gagatgcaga agaaaaatgc cgagaaacag gcgatggaag
    38641 ataacaaatc tgacatcggc tggggcagcc agattcgttc ttatgtcctt gatgactccc
    38701 gcattaaaga tctgcgcacc ggggtagaaa cccgcaacac gcaggccgtg ctggacggca
    38761 gcctggatca atttatcgaa gcaagtttga aagcagggtt atgaggaacc aacatgtctg
    38821 aacaacacgc acagggcgct gacgcggtag tcgatcttaa caatgaactg aaaacgcgtc
    38881 gtgagaagct ggcgaacctg cgtgagcagg gaattgcctt cccgaacgat ttccgtcgcg
    38941 atcatacctc tgaccaattg cacgcagaat tcgacggtaa agagaacgaa gaactggaag
    39001 cgctgaacat cgaagtcgcc gttgctggcc gcatgatgac ccgccgtatt atgggtaaag
    39061 cgtctttcgt taccctgcag gatgtgggtg gccgcattca gctgtacgtt gcccgtgacg
    39121 atctgccgga aggcgtttac aacgagcagt tcaaaaaatg ggacctcggc gacatcctcg
    39181 gcgcgaaagg taaactgttc aaaaccaaaa ccggcgaact gtctatccac tgtaccgagc
    39241 tgcgtctgct gaccaaagca ctgcgtccgc tgccggataa attccacggc ttgcaggatc
    39301 aggaagcgcg ctatcgtcag cgttatctgg atctcatctc taacgatgaa tcccgtaaca
    39361 cctttaaagt acgctcgcag atcctctctg gtattcgcca gttcatggtg aaccgcggct
    39421 ttatggaagt tgaaacgccg atgatgcagg tgatcccagg cggtgccgct gcgcgtccgt
    39481 ttatcaccca ccataacgcg ctggatctcg acatgtacct gcgtatcgcg ccggaactgt
    39541 acctcaagcg tctggtggtc ggtggcttcg agcgtgtatt cgaaatcaac cgtaacttcc
    39601 gtaacgaagg tatttccgta cgtcataacc cagagttcac catgatggaa ctctacatgg
    39661 cttacgcaga ttacaaagat ctgatcgagc tgaccgaatc gctgttccgt actctggcac
    39721 aggatattct cggtaagacg gaagtgacct acggcgacgt gacgctggac ttcggtaaac
    39781 cgttcgaaaa actgaccatg cgtgaagcga tcaagaaata tcgcccggaa accgacatgg
    39841 cggatctgga taacttcgac tctgcgaaag cgattgctga atctatcggt atccacgttg
    39901 agaagagctg gggtctgggc cgtatcgtta ccgagatctt cgaagaagtg gcagaagcac
    39961 atctgattca gccgaccttc attactgaat atccggcaga agtttctccg ctggcgcgtc
    40021 gtaacgacat taacccggaa atcacagacc gctttgagtt cttcatcggt ggtcgtgaaa
    40081 tcggtaacgg ctttagcgag ctgaatgacg cggaagatca ggcgcaacgc ttcctggatc
    40141 aggttgccgc gaaagacgca ggtgacgacg aagcgatgtt ctacgatgaa gattacgtta
    40201 ccgcactgga acatggctta ccgccgacag caggtctggg gattggcatc gaccgtatgg
    40261 taatgctgtt caccaacagc cataccatcc gcgacgttat tctgttcccg gcgatgcgtc
    40321 cggtgaaata agcattacgt tatgctcaca accccggcaa atgtcggggt tttttattta
    40381 agctgggtaa atgcagataa tcgtattctg gcttcgcgat ttgtcgcctg catcaccatc
    40441 cacggactga acgcccacgg cgtggcatca ataccgcgta atacatcagc taaatcacac
    40501 cattgataat ccatcacttc atcatcgttg atctgtaacg cactggtggt gcgtgcggca
    40561 aataccggac acacttcatt ttccacaatg cctctcggat cggtggcgcg gtagcgaaag
    40621 tcaggataga tagattcagg aggcgtaatt tccacgccaa gctcataacg gcaacggcgg
    40681 atcaccgcgt cttcgctgct ttctcccagt tgtgggtgcc cacaaaccga gttagtccac
    40741 acgccaggcc acgctttttt gctcagtgcg cggcgggtaa ctaataattg ccccttggca
    40801 ttaaacagcc aactggagaa cgcgagatgt aagagggtgt ctgccgtgtg tgcggcatac
    40861 ttttccagcg tacccgtggg aactccctgt gcattcaata aaatgacgtg ttccgtttgc
    40921 ataatttctc acatgtaatt ctgatcactc atcattatag ataattgaag agtgaaatga
    40981 ttaaccaaga gagcgtttat cgacaaccgc ccgttttgag cggatgccga caggtaaaaa
    41041 ccatttaaaa aagtagcatc atatatttga agcgactttc aatggccatt gcaacacagt
    41101 cagaaatgac aggaatagag agagtaaaaa gcaacaacag actgaatata gcgcgcatta
    41161 aaaaattcat attattctcc atgctcattt ttcttcagct tgcgcatcag aagccacata
    41221 cgtactgtcc tgacttctaa atctttatca gagacggatt ccttcaccag gtcagcatga
    41281 tgctgatagc cattaaagaa gacgttcagc acaacggcgc taagggttgc gagcataatg
    41341 ccgctatgta gcagcggttg cagtacggcc ggtaacttag aaaagaaatc gtgagagagc
    41401 gtcggagtca tgccaacgcc gagactgatc gccacaatat agaggttgta acggttggtg
    41461 gtgtagttac agcgcgacag aattcgaatc cctgtcgcca gtaccatgcc gaacatcact
    41521 agaccagcgc cgcccagcac aaattgcgga atggaggcta ccagcaccgc catttttggc
    41581 accatgccaa acaggattaa tataattccc gaagaaatac acacccagcg gctatgaacg
    41641 cgcatcacgc taaccaggcc aacgttttga gaaaatgacg tgtgggggaa gctgttaaac
    41701 gtgccgccta tcattgtccc tacgccatcg acacgcagcc cgcaaataat atcgtgtgaa
    41761 gagagtttac gaccgactat ttcacccagt gccaggaaca tccccatcga ctcgataaac
    41821 acgatgatta acacggcagt catggtcaga atggaaacgg gatcgaaaat cggcatacca
    41881 aacgacatcg gggtgacaat cgcaaaccac gaggcatcat gtagcccgga taaattgact
    41941 tcattcatca tccacgaaag taaaaagcca aatacaatcc ccagtaatac ggcgacgttg
    42001 gacataaatc ctttcgcata gcgagtaatg agcaagataa aaattaagac ggcaaaggag
    42061 atacctaaat aaacgggatt accatattgc ggattccctt tacctccagc ggcccagtca
    42121 atacccacct gaatgatgct aagcccaata gaagtaataa ccacaccggt aaccagtggc
    42181 gggaataaag gcatcaagcg accaataagt ggcgctaata atgtggtgat aaaacccgcg
    42241 gcgatagtgg caccaaatat ccccagcagg ccgatatccg ggttcatccc aatggctatc
    42301 attggtgtta cagcagcaaa ggtcaccgac ataatcaccg gcaggcggat ccccataaag
    42361 cggccgatac cgatacattg caataatgtg acgatcccgc agcaaaagag atccgagcta
    42421 atgagcatcg caatagcatc ttttgagagg cccagtcggt caccaatcat aagaggaaca
    42481 gcgactgcac ctgcgtacat gacaaggacg tgttgcagac cgagtatgat cagctttcct
    42541 ggtgataata tgcggtcaac ctcatcagtt gggcggtctt gcccagaaga tgagggaagt
    42601 tgggaatcta tggcgctcat cagtgtttct cctttcgccc gccagaataa aaagcaaccg
    42661 tttacgttaa tgaaactgtc taaacacatg ctcgtggagt gccagttttt gcgaggaagg
    42721 ggagttagag acaggaattg cggaggcaat cacataatcg tgcttaaacc acgaaaatga
    42781 caagagaaac tgacaatgga attatctgta attttctcat attgatattt cattaatcat
    42841 tctggcatgt cgcactctcg catttaatcg tttttatctg gatagcgctc ttttgatcgg
    42901 caatgttgaa ataatgaaag cggcatagat tgaggggcaa taatttgaac tcagtcgcaa
    42961 aattataatt aaaacggtca tgctacctaa tatatgcccg tttattgcat cgcgaaattt
    43021 gagaagacat aactcattaa atgaaaggga tgcaaatgaa tacgtttatc gctgctgaag
    43081 ctgcggaatg tataggctgt ca
//
LOCUS       DAJTSI010000016        43095 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000016 DAJTSI010000000
VERSION     DAJTSI010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43095
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.016"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="OO327_000792"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="OO327_000792"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR6017440.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALALGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="OO327_000793"
     CDS             complement(1672..2730)
                     /locus_tag="OO327_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6017441.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="OO327_000794"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="OO327_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="OO327_000795"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="OO327_000795"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCR6017442.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OO327_000796"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OO327_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR6017443.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSIGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OO327_000797"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OO327_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR6017444.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="OO327_000798"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="OO327_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR6017445.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="OO327_000799"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="OO327_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR6017446.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OO327_000800"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OO327_000800"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR6017447.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OO327_000801"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OO327_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR6017448.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OO327_000802"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OO327_000802"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR6017449.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OO327_000803"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OO327_000803"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR6017450.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="OO327_000804"
     CDS             complement(17699..18322)
                     /locus_tag="OO327_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR6017451.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /locus_tag="OO327_000805"
     CDS             18619..18894
                     /locus_tag="OO327_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6017452.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="OO327_000806"
     CDS             complement(18971..19213)
                     /locus_tag="OO327_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR6017453.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="OO327_000807"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="OO327_000807"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR6017454.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20841..20912
                     /gene="ypdK"
                     /locus_tag="OO327_000808"
     CDS             20841..20912
                     /gene="ypdK"
                     /locus_tag="OO327_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR6017455.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="OO327_000809"
     CDS             complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="OO327_000809"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR6017456.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22592..24078
                     /locus_tag="OO327_000810"
                     /pseudo
     CDS             22592..24078
                     /locus_tag="OO327_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            24093..24827
                     /gene="ypdB"
                     /locus_tag="OO327_000811"
     CDS             24093..24827
                     /gene="ypdB"
                     /locus_tag="OO327_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR6017457.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24840..25697
                     /locus_tag="OO327_000812"
                     /pseudo
     CDS             24840..25697
                     /locus_tag="OO327_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(25700..28195)
                     /gene="ptsP"
                     /locus_tag="OO327_000813"
     CDS             complement(25700..28195)
                     /gene="ptsP"
                     /locus_tag="OO327_000813"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6017458.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28220..29257)
                     /gene="ypdE"
                     /locus_tag="OO327_000814"
     CDS             complement(28220..29257)
                     /gene="ypdE"
                     /locus_tag="OO327_000814"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR6017459.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29257..30340)
                     /gene="ypdF"
                     /locus_tag="OO327_000815"
                     /pseudo
     CDS             complement(29257..30340)
                     /gene="ypdF"
                     /locus_tag="OO327_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30355..31602)
                     /locus_tag="OO327_000816"
     CDS             complement(30355..31602)
                     /locus_tag="OO327_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR6017460.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31624..31950)
                     /locus_tag="OO327_000817"
     CDS             complement(31624..31950)
                     /locus_tag="OO327_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR6017461.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32169..33134)
                     /gene="glk"
                     /locus_tag="OO327_000818"
     CDS             complement(32169..33134)
                     /gene="glk"
                     /locus_tag="OO327_000818"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR6017462.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33338..34594
                     /locus_tag="OO327_000819"
     CDS             33338..34594
                     /locus_tag="OO327_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR6017463.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34709..35035
                     /locus_tag="OO327_000820"
     CDS             34709..35035
                     /locus_tag="OO327_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6017464.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35176..36414)
                     /locus_tag="OO327_000821"
     CDS             complement(35176..36414)
                     /locus_tag="OO327_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR6017465.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36750..37952
                     /gene="nupC"
                     /locus_tag="OO327_000822"
     CDS             36750..37952
                     /gene="nupC"
                     /locus_tag="OO327_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR6017466.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38002..40114)
                     /locus_tag="OO327_000823"
                     /pseudo
     CDS             complement(38002..40114)
                     /locus_tag="OO327_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(40322..40397)
                     /locus_tag="OO327_000824"
     tRNA            complement(40322..40397)
                     /locus_tag="OO327_000824"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40362..40364),aa:Ala,seq:ggc)
     gene            40618..40977
                     /locus_tag="OO327_000825"
     CDS             40618..40977
                     /locus_tag="OO327_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6017467.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            40979..41371
                     /locus_tag="OO327_000826"
     CDS             40979..41371
                     /locus_tag="OO327_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6017468.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41423..42838)
                     /gene="gltX"
                     /locus_tag="OO327_000827"
     CDS             complement(41423..42838)
                     /gene="gltX"
                     /locus_tag="OO327_000827"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR6017469.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
BASE COUNT        11483 a        10724 c         9979 g        10909 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gctaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cagaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacag cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctagcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa acagctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gttgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc atgctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcaaca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aattgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaaact cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggacattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacaactta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caagattaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgat tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aagcgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgataatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attattttat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattaattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ctcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgtgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcttatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccataataac ctcatgattt ttatagcttt tttcttaagt tttttccttc gctgaatatc
    20581 taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa
    20641 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag cgccgtctcg
    20701 agcagtttag ctcagaattc tggggtgatg cccacggtaa aatacagggc ttttaaggtc
    20761 gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
    20821 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg
    20881 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
    20941 ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg
    21001 tttgctgctg acgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc
    21061 ctgacgaata cggtcgtggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc
    21121 gccaaagcca atccccggcg agacacaaac cttcgcttcg ttaaggagct tcttggcaaa
    21181 ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc
    21241 cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata catcacgacg
    21301 gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga tcgccctcga gtgccgcaat
    21361 cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga ttcgcgccag
    21421 ggcactgacc agcgttttgt tgccgaccat aaagccgata cgccagcccg ccatgttgta
    21481 gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt gcacccggca cctgcataat
    21541 tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag
    21601 cacatcgtag catttcgcca gcgccacaac cttttcgaag aactccagtt ccacgcattg
    21661 cgcggttggg ttcgacggga agccgaggat catcatcttc ggtttcggat aactttcacg
    21721 aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac
    21781 ctgcgccccg gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag
    21841 caccgtgtca ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc
    21901 aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca
    21961 gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc
    22021 cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg caccgtccgg
    22081 gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc
    22141 agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg
    22201 gcgagtgtca gccataattt cctcagatta acgttagcgc ccggaccgtc cgagcgacgc
    22261 tgccacaatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta
    22321 gaacattttt atcaatggat ttaaatgagg aactccactt caaaaacagg aaatttttct
    22381 acgctaacaa gaaaatcacc accaatttta ttaacattat aatttcaata aatttaccag
    22441 ccagaagtgc attatcaaga gatacattgc taatacacca tttatctttt aatccccttt
    22501 taaatttatg gtttttcctc aaaaacggcc tgctggtcat cagccttcag gttacctatc
    22561 atagaggttt aatccttatt cagagtcacc cgtgcacgaa atattcaaca tgctgctggc
    22621 ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc tttctcatcc gtatccgcct
    22681 gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa gaattgctcg ccgttaccgc
    22741 cattttctcg ctgttcgccc tgttcagcac ctggtccggc gttcccgtag aaggctcgct
    22801 ggtgaacgta cgtattatcg cggtgatgtc cggcgggatt ctgtttggcc cgtgggtagg
    22861 catcattacc ggcgtgattg cgggtattca ccggtattta attgatatcg gcggcgtgac
    22921 ggcgatcccc tgctttatca ccagcattct ggcgggttgt atatcaggct ggataaacct
    22981 gaaaatcccc aaagcacagc gctggcgctg gatatcgcca acaaaacgct accgctgttt
    23041 cgccatgtca atagcgagtc attacgcaag gtctgcgaaa ttatccgcga tgacattcac
    23101 gccgatgcgg tggcgattac taataccgat catgtgctgg cctatgttgg cgtgggtgaa
    23161 cataactatc agaatggcga tgacttcatt agcccgacta cccgtcaggc gatgaattac
    23221 ggaaaaatca tcattaaaaa caatgatgaa gcccaccgca caccagagac tcattccatg
    23281 ctggtgatcc cattgtggga gaaaggggtc gtgaccggaa cgctgaaaat ttactactgc
    23341 cacgcgcatc agatcacctc gtcattacag gaaatggcgg tcggtctgtc gcaaatcatc
    23401 tccacgcaac tggaggtttc acgcgccgag cagctacgtg aaatggcaaa taaggcagag
    23461 attcgcgccc tgcaaagcaa aattaatccc cattttctgt ttaacgctct gaacgctatt
    23521 tcatcgtcaa tccgtctgaa tccggatacc gctcgccagt tgatctttaa tctgtcgcgt
    23581 tatctgcgct ataacattga attaaaagac gatgagcaaa tcgatatcaa aaaagagctg
    23641 tatcaaatta aagactatat tgccattgag caggcccgct ttggcgacaa gctgacggtt
    23701 atctatgata ttgatgaaga ggtgaattgc tgcattccca gcctgctgat ccagccgttg
    23761 gtggagaacg ccattgtcca cggtattcag ccttgcaaag gtaaaggcgt tgtcaccatc
    23821 agcgttgcag agtgcggaaa tcgggtacgc attgcggtgc gagataccgg gcacggcatc
    23881 gttccaaagg tgattgagcg ggtcgaagcg aatgaaatgc cgggcaataa aattggcctg
    23941 ctgaatgtcc atcatcgcgt gaagttattg tatggcgagg ggctgcatat ccgccgcctg
    24001 gagccgggga cggaaattgc gttttacatt cctaaccaac gcaccccagt cgcctcacag
    24061 gctacgttat tgctttgagc cggagtgata ttgtgaaagt catcattgtt gaagacgaat
    24121 tcctggcaca acaggaactg agctggctaa ttaaagagca cagccagatg gagattgtcg
    24181 gcacctttga cgacggtctg gacgtgttga agtttttgca gcataaccgc gtcgacgcca
    24241 tttttctgga tatcaatatt ccgtcgctgg atggcgtgtt gctggcgcaa aacatcagcc
    24301 agttcgccca taaaccgttt attgtgttca tcaccgcgtg gaaagaacat gcggtagaag
    24361 cgtttgaact ggaggcgttt gactacattc tcaaaccgta tcaggagtca cgtattaccg
    24421 ggatgctgca aaaactggaa gcggcctggc aacaacagca gaccagcagt acgcctgccg
    24481 cgacggtaac gcgtgagaat gacaccatta atctggtgaa agatgagcga ataatcgtca
    24541 cgccaattaa cgatatctat tacgccgaag cgcacgagaa aatgaccttt gtctatacgc
    24601 ggcgtgaatc ctacgtaatg ccgatgaaca ttaccgaatt ttgcagcaaa ctgccgccgt
    24661 cgcatttttt ccgctgccat cgctcatttt gtgtcaatct gaacaaaata cgcgaaatcg
    24721 aaccgtggtt taataacacc tacattctgc gactgaaaga tctggatttt gaagtgccgg
    24781 tcagccgcag caaagtgaaa gaatttcgcc agttaatgca tctttaatcg ggaactttca
    24841 tgaaagcacc aggcttgccc gccgatcaac aatttttcgc cgatctgttc agcggcctag
    24901 tgcttaaccc gcaactactg gggcgcgtct ggtttgccag ccagcctgcc tcgttaccgg
    24961 tgggcagttt atgtattgat tttccccgtc tggatatcgt gctgcgcggc gaatacggca
    25021 acctgctgga agcaaagcag cagcgaatgg tggaaggaga aatgctgttt attccggcac
    25081 gcgcggctaa tttaccgatc aacaacaaac cggtgatgct gttaagcctg gtgttcgcac
    25141 cgacctggct tgggttatcg ttttacgata gccgcaccac gtcgttgttg catcctgctc
    25201 gccagaccca gcttcccagc ctgcaacgcg gtgaaggtga agcgatgctt accgccctca
    25261 cccatcttag ccgttcgccg ctggagcaga atatcattca gccactggtg ttaagtttgc
    25321 tgcatctttg ccgcaacgtg gtgaatatgc cgccgggtaa ttcacaaccg cgcggcgatt
    25381 ttctctatca cagcatttgt aactgggttt aggataatta tgcccagccg ctcacccgcg
    25441 agagcgtggc gcagtttttt aatatcacgc ccaatcatct gtcaaaactg tttgctcagc
    25501 atggaacgat gcgttttatc gagtatgtgc gttgggtgcg aatggcgaag gcgaggatga
    25561 ttttgcagaa atatcatctg tcaattcatg aagtggcaca gcgttgcggt tttccggata
    25621 gcgactattt ttgtcgcgtt ttccggcgtc agtttagtct gacgccggga gagtacagcg
    25681 cccgttttca gggctaacgt cagaaggtta attctgtttc cagcagcgtc aggatacttt
    25741 gtgcatcttg cgcggcaaac aacgactggc ggaagttttt attcaccagt ttacgcgcca
    25801 gctgcgagaa gactttcaca tgattcatcc cttcgttagc acccagcgtc agcatgatca
    25861 ccagttcgac ttcgcccatt tctgactgcc agtcaaccgg tttcgccagc cgggcaatgc
    25921 tgatactgga atgacggatc cactgagatt tggtgtgcgg gatcgctacg ccaaaaccaa
    25981 cgccggtggt aacaatctct tcccgctgcc agacatcttc ttccagctca aacggatgtt
    26041 cagtgcgccc gttaacgccg aggttgccgc acaggaactg gatcgcctgc tctttattgc
    26101 taaaatcctg atcaacaaag atattctcca gcgccagcag tgggcgaacg tcttcttccg
    26161 gcgtaaaggc ggtgagtaac gcttcaattt cctgcgcact gcggcattca catgcctgac
    26221 gcgccagttc ccgacacgcc tcgctatcca gttgacgaag ctggcttttc accgccggaa
    26281 tacgcgggct actcatactc agctcatcca ggcccagccc aagcagtagc ggcagataac
    26341 ggctttcacc gcccagttca ccgcaaatgc ctacccattt gccccgctga tgcgcagtgg
    26401 taactatttg ctgcaacatg cgcaggaacg atggcgtaat cgggttatat agcggcgata
    26461 cgcgcgggtt attacgatcg accgcataca gatactgggt catatcgttg gagccgatac
    26521 tgaagaaatc gacctcatcg cagaagtggt cgatgatgta gcacaccgac ggcacttcca
    26581 ccatgatccc aagcgtaatc gtctctgcat gacgcaggcc atcgcgctta agctcaacga
    26641 tcgctttttg gatctcgcct ttcacccata agatctgatc gaggctgtga accatcggga
    26701 tcatcaactg ggcgttgccg aaactggcgg cgcgcaaaat ggcccgcagt tgagtgcgga
    26761 acaggccagc aaattccggg taaatacgta ccgcgcgata gccgaggaac gggttctctt
    26821 cctggggaat attcagataa ggaatgcttt tatcgccgcc gatgtccatc gtgcggaaga
    26881 taatcggctt gtcgcccgcc gccagtagca cctgctggta ggcttcaaat tgctcctgct
    26941 cgtccggcgc gctgtcgcga tccatataga gcatttcggt acggaacagc ccgacacctt
    27001 ccgcgccgtt ggcaaacgcg cctggcgctt ccagagcggt gccgatattc gccgcaatat
    27061 caatacgctt gttatcacgg gaataggcca gctgcgcggc agcctgcgcc tgttgttttt
    27121 ggcgtttatc cgccagcgtc tgcgcgacct gataataacc gctcaccgcg tcattcgggt
    27181 taatcgccag cacgccgcac tgggcgtcaa gcactgcagg ttgcccggca taacgggcaa
    27241 tcgcatccag cggcaagcca ctcagtaccg ggatcgccga ggcacgggcc agaatcagtg
    27301 tatgcgaggt gcgcccggtt ttctccagaa tcatacccgc aagatttttc aaatcaaggc
    27361 tcaaaaactg gcttggggtt aaatcttcag ccaccagaat ggtcggtttt tccagcacca
    27421 gattgttgcg cggcttcagt tccggccagg tgatatgcag caactgttcg ctgatatcgc
    27481 gaatgtcgct gacccgctcg cgcagataat cgctggcaga agcagatagt ttggcgcaaa
    27541 cctgctccat attgctgatg atcgccgccc ccagtccctg atgctgttct gtcatcaggc
    27601 gacggatatt gcctgcaaat tcatcatcct gaatcagcga caaatgagcg ctgaggatag
    27661 ttttgctttc gccgtcacgc tcacgcaatt gctgattcag ttgctcggca agcgttgcca
    27721 ggctgtgctc cagccgggtg gaatcttgcg cactggcggg gattgcccga taactgtcga
    27781 ggctgtcgct ttgtaacagg gtcagcgtac ccacgccgac gccgcttgcc agcacattgc
    27841 cgtacagtaa atccgggtta aggcggctta atgaacgcgg cagcggatgc gccgtcagtt
    27901 ctgcctgcgt cggctgaacg ctgtcgctgt cgataaagcg caccaggatg tactcttcca
    27961 gcacgcgccg cgcctgctct tcatcgctgc cgctaatgtt caggctgcaa ctgtcattaa
    28021 ataaggtgcc agttccaatc aacgccagcg agcttttagc atcagccttt gcgttctggc
    28081 gatggttgat aaaggtgatt tcgctttgcc actggctgca ctgttcttta agttcccacg
    28141 ccggacgagc atgtagaccg ttaggcaaag gacagagaaa ttgaatcgtt aacataaccg
    28201 ctccgggtta atcaggagat catctgaaat ccgtcagttg aacaaccgtc tcacgcgtaa
    28261 gacgttgaat aagggcagat aaaagttgct ccatctgcaa aatgtcgcgg caatcggcaa
    28321 tcgatgccgc gcaatgtccg tggcgggtgg caggccccat taccaatgtg ggtacgccag
    28381 taccggttaa gtgcaccgct ccaccgtccg tgccgccgtt actgaacata tccgcctgca
    28441 acggaacgcc aatttctgcc gccacggttt caatccacgc ggtaagtttt ggcggcgcaa
    28501 tcagcgattt atcgcttaac accagcatcg gcccgttacc aatctgacga tggttagccg
    28561 cgccataatc aaagtttttc gcccagcagg cggtatcaag cacaatggcg acgtccggcg
    28621 acaccgcgcg ggtagcagtt tgcccgccgc gtaatcccac ctcttcgctg gaacttgcca
    28681 ccagccacac ttccgcaggc agttcggcgt cgtgcagctc acgtagtaac gtcaccagca
    28741 gatagcaact gaggcgatca tcaaaggctt tgcccatcac ccgctggtga gggaggacct
    28801 gaaaagtggt atcgaaggtg acgcgatcgc ctggacgaat tcccgcctgc atcacttcgt
    28861 cataggtgcg cgcgccaatg tccacgcgca tggcgctaac gtcattcccc tgccggtcgc
    28921 cgtcaagcag gcctggaatt ttgcactctt cacgggtggt gatgcgcacc ggctgcagct
    28981 ggcgggcagc catgcgcacg ttgccaaccg gcagcacgtc aatagcgcct tcgcgggaga
    29041 tgctgcgcac cataaatccc acttcgtcca tatgggcgca gatcatcacc ttcggacctg
    29101 tcgattcatt gaggcggatc agcaccgatc ccagaccatc aaatcgcact tctttttgca
    29161 ggcgagccgc ttcttccagc aggatctgcc gcacttcctg ttccgaggag gcgatcgcat
    29221 ctgcctcgct caacgctttt aatagcgata aatccattat gcctctcccg tgagcaacac
    29281 tgttttcggc atggcgtaga gcacttctgc gccttgcggg gtaaccagca caacatcttc
    29341 gatgcgcacg cccccttgcc ctagcaaata aatccccggc tccacggtca gcaacatgcc
    29401 tggctgtagc gtcgtggtgt cccgcggtga aaaacgcgga tcttcatgga cttcaatgcc
    29461 gatagcgtga ccagtgttat gaccgaaata gtcgccaaat ccagcctcgg taatgacccg
    29521 gcgcgcggcg tcgtcaatct gctggcagcg cacgccgggg cgaattgcag agattgccgc
    29581 gagctgtgcc tgcaggacaa tctgatagac gtcaaacagc gggtgagatt cggcgctcac
    29641 cccttcgcca ttcaccagca aggtgcgcgt catatcagca gtagccctga tacagcgcac
    29701 caaaatcgag agtgacaaac tcgcccgctg caacaatctt gtcgctggct ttgccgtgcg
    29761 gcagcgcccc gcgccagccg ctggcgacaa tggtgtcaaa agaggctttt tctgcgccct
    29821 gctggcgcat aaaccactcc agttcagcgg ctatctcgcg ctcgctcatc cccgcctgaa
    29881 taaagcggcg aatatgctcc gctccgcgat cggcaatccc acaggcgagg cggattatct
    29941 ccacctcctc tggcgttttg atttgccgca gcacatccgg cgtggcgcta accagtttcg
    30001 cattgagttc agacttccag cggtgcgcgg tttcccaact cacctgctgg ccttcaaaac
    30061 cgagcgtttg caactgctca tcggcaatga tttgattgac gatagtggta agcgtgttcg
    30121 ttgcgtcaag caaatgcagc tggtagcctt gcgcacgggc ttccacctca acgaaatagc
    30181 gcgaatctac cagaatgtgc gcactttcac ggctaataac cacatagcct gatccggtgg
    30241 agatccccag atgcggctgt ttgttctgcc gtgaggagag aagcaccgca tccagttgtt
    30301 gcaccttaag ccagttgcgc agcgaagcga gtaatgtcat ctggattttc cttcttacag
    30361 gctatcgatt aacaatttgc ctttgcggaa catcatcaga cgcaggaaca ccaccatcag
    30421 cgcagtaatg attgccccca gcgcgatccc cgccatataa acgcccaagt tagtgaccag
    30481 cggccatgcc cagatagcgg attccgggaa ccattgcact gcgcccagcc agacagctgc
    30541 ggttgagccg acaatcgcgc cgaccatata tgacggaata gcggtgatgg ggctttccag
    30601 cgcaaacgga atcgcaccct cactaatccc cataaaggcc aggaacatcg cggtcttacc
    30661 ctgcggataa agttgagcgt tgaacaggcg tttgccggtt aaacgacggt caataatggt
    30721 cgccagaccc agaccaatcg gcggaataac gatagcgata gaacgagcgg tgaccggtaa
    30781 tacgtggtca gtggtaaagc tgaaggcaac gaaacctgcg gctttgttga tcgggccacc
    30841 aaggtcgatt gctgtcgccg cggcgatccc cattgcgtac atcagcgcgc ccttctcacc
    30901 tgcggcagtc agcacagtac ggataccgcc gttgatccag ccaccaaagg gagtgatgac
    30961 gtagtacatc gccagcatga caaaaatggc ggaaagaatc ggtagcaaaa atgtggtttt
    31021 gaacgctaac aggaaatccg gtaactggat tttctggttc atccatttca caaggtaacc
    31081 ggcaacgata gaaataatca gcgcaccaat gaaggtggac ggcaccggcg aagaggtcgc
    31141 ccattgcata gtgctgggat cgaagttcag cagttgcgtc ggctgggtag acatcaaccc
    31201 gccgataaaa ccagccggga acgccagttt gccaccgata gagttcgcca caaatgcagc
    31261 aaacatcgga atggcaaaac caaacaacac gccgccgaag gactgcgaca gccaggcaaa
    31321 tttaagtaag gagaggtcga aaccggagaa tttcccgcta ttaagagcat ccatgatgcc
    31381 gatctcagca ggaattttaa gccagctata agcaatcagc tggctgaaag cgaggatcac
    31441 accgcccata attaaggtcg gcaccatgcg tgaaatccct gacatcacat gctgcgggag
    31501 ttcaccccag aagctgcttt tagaggcctg cggattctta actgccgctg ccgcaccgga
    31561 tgcgccaggc acaacggttg cactgcgttt tttaatggcc ataacaatta tccctgtcga
    31621 atattactgt tgttcagagg caatcatctc ttcgatttct ttgatgatgc ccgcagcgtt
    31681 tttaattgcg tcctgcaaag tgatttcata aacgtcgcgt gattcgaaac gttcgttatc
    31741 ttccggggta actgccacgg aatggatgat gatggtcgct tcggcgatat cctgcgccgt
    31801 caggcgattc tggatgccgt ccgcgccctg ggtttcaatt ttcacttcat aaccggcttc
    31861 taccgccgct tcttccagca cctgagcggc cataaaagtg tgagccaggc ccattgggca
    31921 ggcacataag gcaatcagtt tcttactcat cgttaattcc tcgttcagtg atagttgact
    31981 agtgaaatta tgaacgtgca aaacaaatcg ccgttaccag acaaataatg catttactgg
    32041 agaattaatc ccgctgtacg gaagtgtgac gcaggtcggc cttgtgaggg gacacaaaga
    32101 aaaattgaat taactgtatg atttaaaaga ttatcgggag agttacctcc cgatataaca
    32161 ggaaggattt acagaatgtg acctaaggtc tggcgtaaat gtgcaccgga accgagaaga
    32221 cccggatagt catggacgat gagatacacc ggaatatcat ggacatattc tttaaagcgc
    32281 cctttatctt caaatgcggc acggaaaccg gaggctttga agaactcaag gaagcgcggc
    32341 acgataccgc ccgcaataaa cacgccgcca aatgtcccga gattgagcgc cagattgccg
    32401 ccaaaacggc ccataatgac gcaaaacagc gacaatgcgc ggcggcaatc ggtgcagctg
    32461 tcagccagcg cgcgttcggt aatatctttt ggcttgagat tttctggcag gcggttgtca
    32521 gctttcacaa ttgcgcgata caaattcacc agcccagggc cagaaagcac gcgctccgcc
    32581 gaaacatgac caatttccgc acgcaatatt tcgaggataa tggcctcttc ttcactattc
    32641 ggcgcaaaat caacgtgacc gccttcgccc ggcaagctta cccaacgctt atcgacatgg
    32701 accagatgcg caaccccaag ccccgttccg gcaccgtaaa ccgcaatagg cttaccttcg
    32761 accggttctg cgccaccaaa ctgaatcaga tgctcttttt tcagcatcgg gatcgccatc
    32821 gatacagcgg taaaatcgtt aataatttcc agatggctaa aaccgagatt ctttttcatt
    32881 tcggcaattg agaacgccca ggtatggttg gtcattgcca cccagtcacc ggtaattggg
    32941 caagcgatgg caatacagcc gtctttcacc tcgaccttat gttcttcaag ataaacgcga
    33001 atgaccgctt cgaggctggg gtaatcaagc cctgaatagg tcttagcctg cgagatttca
    33061 ccactggcaa tatcacacag agcaagacgt gcgttggtgc cgcccacatc accgactaat
    33121 gcatactttg tcattcttca actgctccgc taaagtcaaa ataattcttt ctcacactgt
    33181 aaatacctgg gggcataaca acaacgccgg aaaggcaggc tccctgtaaa tatcgatctg
    33241 ggtcacacaa ttactttatc gtttcagcac caattgcagc gatgcctttt tgcaagctgg
    33301 gcaaactaag tatctgaccc cgcataagga atagaacatg ctccatccgc gagccagaac
    33361 catgttgtta ttatcgctcc ccgccgtggc aattgggatt gcgtccagtc ttattctgat
    33421 tgtggtgatg aaaatcgcct cggtattaca gaatttgctc tggcaacgac tgccgggaac
    33481 tctggggata gcccaggatt cacccctctg gatcatcggt gtattaacgc taacgggtat
    33541 tgcggtgggg ttggttatcc gtttcagcca gggtcatgcc ggaccagacc ccgcctgtga
    33601 accgctgatc ggcgcaccgg ttccgccctc tgcgctacct agacttatcg tagcattaat
    33661 tctcggtctt gctggcggcg tcagcctggg gccggaacat ccgatcatga ccgtcaatat
    33721 cgcccttgcc gtcgccattg gcgctcgtct gttaccgcgc gtcaaccgaa tggagtggac
    33781 tattttagcc tctgccggaa ccatcggcgc gctgtttggt acacctgttg cggcggcgtt
    33841 gatattttcg caaaccttaa atggcagtag tgaagttccg ctatgggatc gtctttttgc
    33901 gccgttaatg gcggcagcag ctggtgcact tactaccgga ttatttttcc atccccattt
    33961 ttcactgccc attgcgcatt acggacagat ggaaatgacc gatattctca gcggtgcaat
    34021 tgtcgcggcg attgccatcg cagcagggat ggtcgccgta tggtgcttac cacggttgca
    34081 cgcgatgatg aatcaaatga aaaatccggt gctcgtgctg ggtattggcg gatttattct
    34141 cggaattctg ggggttattg gtggaccagt ttcgctgttt aaagggctgg atgagatgca
    34201 gcagatggtg gcaaatcagg ctttcagcac cagcgattac tttttgctgg cggtaattaa
    34261 acttgccgcc ctggtcgttg ctgccgccag tggctttcgc ggtgggcgaa tcttcccggc
    34321 agtgtttgtc ggcgtggcat tagggttgat gctgcatgag cacgttcccg ccgttccagc
    34381 ggcaataacc gtttcctgcg ctattctcgg catcgtgctg gtggtaacac gcgatggctg
    34441 gttaagtctt tttatggcgg cagtcgttgt acccaatacc acattgctac cgctgctctg
    34501 tatcgtcatg cttccggcat ggctgttatt agcaggtaag ccgatgatga tggtcaatcg
    34561 tccgaagcaa cagccacccc acgataatgt ttagcaaaaa atgctctcct ttatgattta
    34621 agagttatgg cgatgatacg gggctttacc tccccgtaat attgcgttaa caggccgctg
    34681 acggcttcga tcgtgaagga gaataacaat gttcaggtca ctgtttctgg cggccgccct
    34741 gatggcattt accccgcttg cagcaaatgc aggtgaaatc accctactgc catcgatcaa
    34801 attacaaatt ggcgatcgcg atcattacgg taattactgg gacggtggtc actggcgaga
    34861 tcgtgattac tggcatcgca attatgaatg gcgcaaaaac cgctggtggc gtcatgataa
    34921 tggctatcat cgtggctggg ataagcgtaa agcatatgag cgtggctatc gtgaaggctg
    34981 gcgcgatcgt gacgatcatc gcggaaaagg ccgcggacat gggcaccgcc attaaacgcg
    35041 tcctccaatg gagcacaatg cctgatgcga cgctagcgcg tcttatcagg cctacaaacc
    35101 ggctctttaa cgcgtagggc ggataaggcg tttacgccgc atccgccaat agtgccagat
    35161 gcgacgctca ttcaactaca atcccagcgc cgtccccacc aacaaccaga tattcagcgc
    35221 cacgaccagc actacaatca cccagcctgt ctgttttacg cgtttgctgt tcaccagatc
    35281 gcccatcaac ttgctgtcac tggtgaaaat cagcagtgga accagcgcca gggcgatacc
    35341 aaaacttaac agcacctgac tcataaccag aatccgtgtc ggatctaatc ccatcagaat
    35401 gacaataaat gacggcaaca tggtgactgt acgacgcacc cacagcggga tatggaagcg
    35461 aatgaatccc tgcatcacca cctgccccgc cagtgtcccc accaccgttg aggacagtcc
    35521 ggcagcaacc agacttaacc caaagaccgt tgccgcagca tggcttaaca gcggttgcag
    35581 cgtcagataa gcctcatcaa gatcggcaac accagtatga ccagaaaagt ggaacgccgc
    35641 cgcagctgta gccatcatca ccagattgac aaaaccggca atcgtcatgg cgatagccac
    35701 atcccatttg gtggcggaat aacgttgttg acgcgaaccg ccatgtaaat gctgagtgag
    35761 cgaagagtgc aaataaatca catgcggcat aatcgtcgcc cctaacacgc ctgctgccag
    35821 gaagaccgcc tccgaagtag gtaaactcgg gatcaccatt cctttaccca gctgcgccag
    35881 gttaggctgg gagaaaatca actcgacaat gtaagccgcg gcaacaaaca acagtaaccc
    35941 gccaatcact ttctccagcg gtttttgccc gcgacgttgc agcattaaaa tcaggaaagt
    36001 cgcgatcccc gtcagcaccg cgccctgcaa caacgaaaca ccaagaatga gtttaaaacc
    36061 gatcgccgca ccaataaatt ccgccaggtc ggttgccatc gcaataattt ctgcctgaac
    36121 ccaatagaac cacactacgg gacgcggata gtgatcacga atctgctccg ccagattttt
    36181 accggtggca atccctagtt tggcagagag gatctgaatc agcatcgcca tcaggttggc
    36241 ccaaacgaca acccacagta gctgatagcc gaagctagca cccgcctgaa tattggtcgc
    36301 aaagttaccg ggatcgatat aaccaatcgc cgcaatgaac gcaggtccca ttaatgcgag
    36361 cctcatctta cgcgccgccc gtccgctgct actctcaacg cgatagttcg tcatcttgtg
    36421 cctctaaaac atagcctttg ctatgtttca tgctatgcta aacgagaatg attatcaaat
    36481 tcatttaaat ggattgtggt gatttctctg atagaccaag attatgatta cgaaaagatt
    36541 gatgacttga atgttatggt tatgtttaat gttagcacat ttacataact ttcagcttcc
    36601 atacacaaca tagcagaaat gtatgacaga tcactatttt tgaagcctgt cacaggacgt
    36661 cattatagtg tgtgtcagat ctcgttttcc ttaaccatgt tacatagaat gtgcacggaa
    36721 atttaacctg cctcatattt ggagcaaata tggaccgcgt ccttcatttt gtactggcac
    36781 ttgccgttgt tgtgattctc gcactgctgg taagcagcga ccgcaaaaaa attcgtatcc
    36841 gttatgttat tcaactgctt gttatcgaag tgttactggc gtggttcttc ctgaactccg
    36901 acgttggttt aggcttcgtg aaaggcttct ccgaaatgtt cgaaaaactg ctcggatttg
    36961 ccaacgaagg gactaacttc gtctttggta gcatgaatga tcaaggcctg gcattcttct
    37021 tcctgaaagt gctgtgccca atcgtcttta tctctgcact gatcggtatt ctccagcaca
    37081 ttcgcgtgtt gccggtgatt atccgcgcaa ttggtttcct gctctccaaa gtcaacggta
    37141 tgggcaaact ggaatccttt aacgccgtca gctccctgat tctgggtcag tcagaaaact
    37201 ttattgccta taaagatatc ctcggcaaaa tctcccgtaa tcgtatgtac accatggctg
    37261 ccacggcaat gtccaccgtg tcgatgtcca tcgttggtgc atacatgacc atgctggaac
    37321 cgaaatacgt cgttgctgcg ctggtactga acatgttcag cacctttatc gtgctgtcgc
    37381 tgatcaatcc ttaccgtgtt gatgccagtg aagaaaacat ccagatgtcc aacctgcacg
    37441 aaggtcagag cttcttcgaa atgctgggtg aatacattct ggcaggtttc aaagttgcca
    37501 ttatcgttgc cgcgatgctg atcggcttta tcgccctgat cgccgcgctg aacgcactgt
    37561 ttgccaccgt tactggctgg tttggctaca gcatctcctt ccagggcatc ctgggctaca
    37621 tcttctatcc gattgcatgg gtgatgggtg ttccttccag tgaagcactg caagtgggca
    37681 gtatcatggc gaccaaactg gtttccaacg agttcgttgc gatgatggat ctgcagaaaa
    37741 ttgcttccac gctctctccg cgtgctgaag gcatcatctc tgtgttccta gtttccttcg
    37801 ctaacttctc ttcaatcggg attatcgcag gtgcagttaa aggcctgaat gaagagcaag
    37861 gtaacgtggt ttctcgcttc ggtctgaaac tggtttacgg ctctaccctg gtgagtgtgc
    37921 tgtctgcgtc aatcgcagca ctggtgctgt aaaaccatac ataaaaaagc cggggatcat
    37981 tccccggctt ttttacgcct gttaatcagc taatggctgc gggcgaccaa tcaaataccc
    38041 ttgcagatat tgcaccccga gcttatgcaa tagcgcctgc tgctgttgcg tctcgacaaa
    38101 ctccgcgacc acactcaatg acttcgcttt cgccagatcg gtaattgatc tcacaatcat
    38161 cgcatccagc gtattcgtga caatatcttt cacaaagacg ccatcaattt tgatgatatc
    38221 agcctgcaaa cgctttaacc gttcgtagtt ggcatatccg gtgccaaagt catcaatcgc
    38281 aatccggaaa ccaaacttat gcagctgctc gatgttgtac atgctgcttt ctgcgttaga
    38341 aaacgcctgc tcctcggtga tctcaagaat gaccgcctgc ggggagatgt gataacgctt
    38401 aaacagacga ataatccgcc cggcaatatt cttttgcagc agcgtgagcg gcattaaatt
    38461 gactgaaaag cgcggtcctt ttttgtcgca agggtgtgtt gccagccact tcaacaagga
    38521 ttccagcact tgcaaatcaa aacgcgcgct aaggttaaac tgagcaataa ggggcagaaa
    38581 cttatccggg gtcataatgc cgccgtcata tttcagtcgc gcgaggatct catcataacc
    38641 ttcaccctct ttgttgcgaa ttggctgggc gtagagcagc aaatcaccct gatctaacgc
    38701 cgtgcgaatg gtattcagca atagcacctg tttagtggtc tgcccggaaa ccatctcctc
    38761 tctgctatcc agcgccagca catgatgatg tgcgcaggat tgctccgcca gccagcttaa
    38821 ctgccccaac aagggttgca gggtttcctg atttccatca aaacgccccc aggcagcacc
    38881 atagcccata tccagcccgg tattgttcca gtgaatttgc cgactattca ggatgttaac
    38941 catatgctgg agtcgccctt ccgtttccgg cccgctcagc actaacagca gttcactacc
    39001 cggcaattga tacaactttt cgttttcctg catcaacggc agcagcgtac ggcaaattga
    39061 gcggatacag tgaacgcgca tcattaagcc gtaatgacga ctcataaact caagattatc
    39121 aatgcgcagg cagcaaaaac tcttgcccgc ctcttgctcc ggcgcttgtt ccaacgcacg
    39181 aaagttgggt aggagcgtta acggatctgt tagcgcctgc aaatgccagc ggcgattaag
    39241 ccattcactg cgatgataaa tgcgcaccat atagagcagg caaacgctaa aggaaatcag
    39301 caccgcgaga ataaatgcca gcgaatattc ggtttccacc ccttgcaaaa agttctgatt
    39361 gtaattcaga aggcaaagcg ttgaaacagc ccaggtgaga tttaaaaacg gatagcgaag
    39421 attaccgacc ccgagggtaa agatgatgaa gaaaacgggt accaggtaac cggcaataaa
    39481 gtcgttttca taaggtgtgc acaacagaag tagcagcacg cttaacgctg ccagccagct
    39541 taaggtaaat gcgagtgaga tagtagaaaa gcatgttgta aatcagcact gcggtgaaaa
    39601 ggcttagtaa atcaacgacc gtgaaaatgg catccgcatc gccgaaaaag gtggatatct
    39661 ttagcgggaa atcaaagaag ctccccacaa gatacatgct gcatttgatg ccgatcggcg
    39721 tcaccaaacc aagccagaac agacgttgcc agacatatcg gctggtcaat ccataacgcc
    39781 aacgcgtccc cagctgccag cgtaatattg cgcaggcaca caacacgaca aacgtctggc
    39841 tgccgagcat gaccgcagtc tgtaaaaaag agagatgaaa gttccacaga ttggtgcaaa
    39901 acatcccgca taaaacggga acgacaccgc gccagccaaa gataaacaag attgacagca
    39961 ttacgcacag tggcatccac gccagaaaga tataactgga atggataatg gcaagtggcg
    40021 aaataaaacg ggatagctga ataagtacca cggtgagcgt aaacgctagt gtgaatatct
    40081 tgatattttt tatcaggtta tgctccacaa acatgagtac actactctca ctgatcgggg
    40141 ttttcaggca cacaaataat aagtttattc gcatcgcgaa gcaaacaggg gccagcctgt
    40201 tgccgatttt tcatccaatg atgctttcac caggcggcgc taacccggac gtagactgta
    40261 ttacaaaagc ggcaaaaagc agagacaaaa aacccccgct ttgcagcgag ggttggaaat
    40321 ttggtggagc taagcgggat cgaaccgctg acctcttgca tgccatgcaa gcgctctccc
    40381 agctgagcta tagccccgtc acgtaaagct tgtcgagttg acgggcggca tcatatgaat
    40441 tccgcccgaa tgtgtcaacg gcaaattgca acgtgtagtt tcaatcgctg aaaaatcagg
    40501 caaatgaaca attttggaaa accgctcgca ttcagtagtt attcatgtca cggtttcctg
    40561 taaagtggtg ttataaaatg aactactaat agacccacat acattcaggg aattgttatg
    40621 ttcaaggaga ggatgacacc agatgaactt gccagactga ccggctatag ccgccagacc
    40681 attaataaat gggtacgcaa ggaaggctgg acaacgtcac caaaacctgg cgtccagggt
    40741 ggcaaagccc gactggttca cgtcaatgaa caggttcgtg aatatattcg caatgctgaa
    40801 cgtccagaag gtcagggaga agcgcctgcc ctttctggtg atgcgccgct tgaagtattg
    40861 ctggtgacac tggcgaaaga gatgacgcca gttgaacaaa aacagtttac atccttgctt
    40921 ctgcgggaag ggattatcgg attgttacaa cgcttaggga ttcgcgatag caaataatat
    40981 gaaaagatta cgcaataaaa tgaccaccga agaactggct gaatgcctcg gtgtggctaa
    41041 acaaactgtt aaccgttgga ccagagaaaa aggctggaaa acggaaaaat ttcccggcgt
    41101 gaaaggcggt cgtgccagac ttattctggt cgatacgcaa gtttgcgagt ttattcagaa
    41161 cacgccagcc ttccataaca caccaatgtt gatggaagct gaagagagaa ttgctgaata
    41221 cgctcctgga gcacgcgccc cggcgtatcg acaaatcatc aacgcgatag ataatatgac
    41281 agacattgag caggaaaaag tcgcgcaatt tttgtcacgt gaaggaattc gcaatttcct
    41341 cgcccgtctc gatattgacg aatcagcata aataaaaaac ggcaggatat tatctcctgc
    41401 cgtttatctt tttacacgct aattactgct gattttcgcg ttcagcaata aaatccagcg
    41461 ctttgttgat acgctcgata ctgcgggtct taccaattgc gtgaacggta acatccagtg
    41521 ctggagactg ccccgcaccg gttacggcga cacgcagcgg cataccaact ttacccatac
    41581 ccacttccag ctcatccgcc gtcgcctgaa tagcgtgatg aacgttttca gcggtccagt
    41641 cagtaatcgc ggccagtttg tcacgaacca cttccagcgg ctgacgcgct accggacgca
    41701 gatgtttttt cgccgcatcg gcatcgaact cagcaaaatc ttcgtagaaa taacggcagc
    41761 tctgtgccat ctctttcagc gtcttgcagc gttcgcccag cagtttcacc agatcagcca
    41821 gctgcgggcc gttacgggta tcgatatttt cctgctcaat gtgccactgt aagtgagtag
    41881 caacatactc cggcggcagc gcgttaatgt agtgatggtt cagccacagc agcttgtcgg
    41941 tgttgaacgc actggcagat ttgctgacgg cattcaaagt gaagtatttg atcatctctt
    42001 cacgagtgaa gatttcctga tcgccgtggg accagcccag acgcaccaga tagttcagca
    42061 gtgcttctgg caaataaccg tcatcacgat actgcattac gctgactgcc ccgtgacgtt
    42121 tggacagttt tttaccgtca tcgccattga tcatagaaac gtgcgcgtaa accggcaccg
    42181 gcgctttcag ggccttaaga atgttgatct ggcgcggcgt gttgttgata tggtcttcgc
    42241 cacggataac gtgggtgatt tccatatccc agtcatcgac aaccacacag aagttatagg
    42301 ttggggaacc atcggtacgg cggataataa gatcgtccag ttcctggttg ctgaactcga
    42361 tcggaccacg gatctgatcg tcaaaaacaa cagaaccttc ctgcgggtta gcaaaacgta
    42421 caacacacgg ttcatcatca gcatgatgct catggctgtg gcggcagcga ccgtcataac
    42481 gcggcttctc acctttcgcc atttgctctt cgcgcagcgc ttccaggcgc tctttagagc
    42541 aatagcattt ataagcagtg ccctcttcca gcatctgatc gatcaccgcg ttgtagcgat
    42601 caaaacgttt ggtctggtag tacggacctt catcccactc caggctcagc cagttcatgc
    42661 catccataat ggcttcgata gcttccggcg tggaacgctc aagatcggtg tcttcaatac
    42721 gcagcacgaa ctcaccgccg tggttacgtg caaaaagcca ggagtaaaga gcagtacgcg
    42781 cgccgccaac gtgcagatag cctgttgggc ttggcgcgaa gcgagttttg attttcatga
    42841 aatggcctta cgtttagaaa gatgccgaca accggcaaat cctggaaaaa ttgagtgggc
    42901 gatattctat cactcccgcc tgattcctca atgtagttcg ggtttctacc ttacgcatta
    42961 ttgttttcgt tgacaaattg cgcaacacgg tctgttttgc gatcatcttg cttaaattta
    43021 cgacgaacga acaatttctt tagaaaatgc gttgactcat tttgaactct ccctataatg
    43081 cgactccaca cagcg
//
LOCUS       DAJTSI010000017        42970 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000017 DAJTSI010000000
VERSION     DAJTSI010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42970
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.017"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(72..476)
                     /locus_tag="OO327_000828"
                     /pseudo
     CDS             complement(72..476)
                     /locus_tag="OO327_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
     gene            complement(660..2078)
                     /gene="gltD"
                     /locus_tag="OO327_000829"
     CDS             complement(660..2078)
                     /gene="gltD"
                     /locus_tag="OO327_000829"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR6017470.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            complement(2091..6551)
                     /gene="gltB"
                     /locus_tag="OO327_000830"
     CDS             complement(2091..6551)
                     /gene="gltB"
                     /locus_tag="OO327_000830"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR6017471.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            7226..8155
                     /locus_tag="OO327_000831"
     CDS             7226..8155
                     /locus_tag="OO327_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR6017472.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            8251..10587
                     /gene="arcB"
                     /locus_tag="OO327_000832"
     CDS             8251..10587
                     /gene="arcB"
                     /locus_tag="OO327_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR6017473.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            10817..11470
                     /gene="elbB"
                     /locus_tag="OO327_000833"
     CDS             10817..11470
                     /gene="elbB"
                     /locus_tag="OO327_000833"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR6017474.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            11467..12194
                     /gene="mtgA"
                     /locus_tag="OO327_000834"
                     /pseudo
     CDS             11467..12194
                     /gene="mtgA"
                     /locus_tag="OO327_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(12191..12823)
                     /gene="yrbL"
                     /locus_tag="OO327_000835"
     CDS             complement(12191..12823)
                     /gene="yrbL"
                     /locus_tag="OO327_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR6017475.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(13036..13308)
                     /gene="npr"
                     /locus_tag="OO327_000836"
     CDS             complement(13036..13308)
                     /gene="npr"
                     /locus_tag="OO327_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR6017476.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            complement(13305..14159)
                     /gene="rapZ"
                     /locus_tag="OO327_000837"
     CDS             complement(13305..14159)
                     /gene="rapZ"
                     /locus_tag="OO327_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR6017477.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            complement(14205..14696)
                     /gene="ptsN"
                     /locus_tag="OO327_000838"
     CDS             complement(14205..14696)
                     /gene="ptsN"
                     /locus_tag="OO327_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR6017478.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            complement(14814..15101)
                     /gene="hpf"
                     /locus_tag="OO327_000839"
     CDS             complement(14814..15101)
                     /gene="hpf"
                     /locus_tag="OO327_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR6017479.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            complement(15124..16557)
                     /gene="rpoN"
                     /locus_tag="OO327_000840"
     CDS             complement(15124..16557)
                     /gene="rpoN"
                     /locus_tag="OO327_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR6017480.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            complement(16605..17330)
                     /gene="lptB"
                     /locus_tag="OO327_000841"
     CDS             complement(16605..17330)
                     /gene="lptB"
                     /locus_tag="OO327_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR6017481.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            complement(17337..17894)
                     /gene="lptA"
                     /locus_tag="OO327_000842"
     CDS             complement(17337..17894)
                     /gene="lptA"
                     /locus_tag="OO327_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR6017482.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            complement(17863..18438)
                     /gene="lptC"
                     /locus_tag="OO327_000843"
     CDS             complement(17863..18438)
                     /gene="lptC"
                     /locus_tag="OO327_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR6017483.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            complement(18435..19001)
                     /gene="kdsC"
                     /locus_tag="OO327_000844"
     CDS             complement(18435..19001)
                     /gene="kdsC"
                     /locus_tag="OO327_000844"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR6017484.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            complement(19022..20008)
                     /gene="kdsD"
                     /locus_tag="OO327_000845"
     CDS             complement(19022..20008)
                     /gene="kdsD"
                     /locus_tag="OO327_000845"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR6017485.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            complement(20022..20987)
                     /locus_tag="OO327_000846"
     CDS             complement(20022..20987)
                     /locus_tag="OO327_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR6017486.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            21197..22006
                     /gene="mlaF"
                     /locus_tag="OO327_000847"
     CDS             21197..22006
                     /gene="mlaF"
                     /locus_tag="OO327_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR6017487.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            22014..22796
                     /gene="mlaE"
                     /locus_tag="OO327_000848"
     CDS             22014..22796
                     /gene="mlaE"
                     /locus_tag="OO327_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR6017488.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            22801..23352
                     /gene="mlaD"
                     /locus_tag="OO327_000849"
     CDS             22801..23352
                     /gene="mlaD"
                     /locus_tag="OO327_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR6017489.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            23371..24006
                     /gene="mlaC"
                     /locus_tag="OO327_000850"
     CDS             23371..24006
                     /gene="mlaC"
                     /locus_tag="OO327_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR6017490.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            24006..24299
                     /gene="mlaB"
                     /locus_tag="OO327_000851"
     CDS             24006..24299
                     /gene="mlaB"
                     /locus_tag="OO327_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR6017491.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            24459..24713
                     /gene="ibaG"
                     /locus_tag="OO327_000852"
     CDS             24459..24713
                     /gene="ibaG"
                     /locus_tag="OO327_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR6017492.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            24768..26027
                     /gene="murA"
                     /locus_tag="OO327_000853"
     CDS             24768..26027
                     /gene="murA"
                     /locus_tag="OO327_000853"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR6017493.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(26075..26353)
                     /gene="sfsB"
                     /locus_tag="OO327_000854"
     CDS             complement(26075..26353)
                     /gene="sfsB"
                     /locus_tag="OO327_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR6017494.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(26580..27551)
                     /gene="ispB"
                     /locus_tag="OO327_000855"
     CDS             complement(26580..27551)
                     /gene="ispB"
                     /locus_tag="OO327_000855"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR6017495.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            27809..28120
                     /gene="rplU"
                     /locus_tag="OO327_000856"
     CDS             27809..28120
                     /gene="rplU"
                     /locus_tag="OO327_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR6017496.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            28141..28398
                     /gene="rpmA"
                     /locus_tag="OO327_000857"
     CDS             28141..28398
                     /gene="rpmA"
                     /locus_tag="OO327_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR6017497.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            28525..29490
                     /locus_tag="OO327_000858"
     CDS             28525..29490
                     /locus_tag="OO327_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6017498.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            29506..30678
                     /gene="cgtA"
                     /locus_tag="OO327_000859"
     CDS             29506..30678
                     /gene="cgtA"
                     /locus_tag="OO327_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR6017499.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(30864..32297)
                     /gene="dacB"
                     /locus_tag="OO327_000860"
     CDS             complement(30864..32297)
                     /gene="dacB"
                     /locus_tag="OO327_000860"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR6017500.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            32545..33021
                     /gene="greA"
                     /locus_tag="OO327_000861"
     CDS             32545..33021
                     /gene="greA"
                     /locus_tag="OO327_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR6017501.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            complement(33177..33470)
                     /gene="yhbY"
                     /locus_tag="OO327_000862"
     CDS             complement(33177..33470)
                     /gene="yhbY"
                     /locus_tag="OO327_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR6017502.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            33596..34225
                     /gene="rlmE"
                     /locus_tag="OO327_000863"
     CDS             33596..34225
                     /gene="rlmE"
                     /locus_tag="OO327_000863"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR6017503.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            34325..36259
                     /gene="ftsH"
                     /locus_tag="OO327_000864"
     CDS             34325..36259
                     /gene="ftsH"
                     /locus_tag="OO327_000864"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR6017504.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            36349..37197
                     /gene="folP"
                     /locus_tag="OO327_000865"
     CDS             36349..37197
                     /gene="folP"
                     /locus_tag="OO327_000865"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR6017505.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            37190..38527
                     /gene="glmM"
                     /locus_tag="OO327_000866"
     CDS             37190..38527
                     /gene="glmM"
                     /locus_tag="OO327_000866"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR6017506.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            38755..39087
                     /gene="secG"
                     /locus_tag="OO327_000867"
     CDS             38755..39087
                     /gene="secG"
                     /locus_tag="OO327_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR6017507.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            39102..39188
                     /locus_tag="OO327_000868"
     tRNA            39102..39188
                     /locus_tag="OO327_000868"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39136..39138,aa:Leu,seq:gag)
     gene            39666..41273
                     /locus_tag="OO327_000869"
                     /pseudo
     CDS             39666..41273
                     /locus_tag="OO327_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(41281..42624)
                     /gene="argG"
                     /locus_tag="OO327_000870"
     CDS             complement(41281..42624)
                     /gene="argG"
                     /locus_tag="OO327_000870"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR6017508.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
BASE COUNT        10177 a        10585 c        11511 g        10697 t
ORIGIN      
        1 ttgcatgaat atacaaaccg aaaataggag atgcaataca aaagctgtgg aaatatcaaa
       61 cagcctctca atttggcaga cagcgtctgc caaatctttg ccgatgggaa ttctgcagat
      121 ggtgtctgcg gaattcccaa tggtgttaat tcgccagaca ctgtctggcg aatttcaaca
      181 tgttgaaaaa agaggtaaaa aagcctcatt tggcgtaagt tttttgcaga gaccccacgt
      241 ttataccgtc gacttaaatc cttcgatagc cgatcaatca actgtgcacc ataagctgct
      301 ctggcctcac caccttgttc aaattcgaca atccgtcggc caatttccca gtacgtcgcg
      361 gtcatcagcg cgttaacgct acgtaccgtt tccgttcggg cgctatcgac cagatgaata
      421 atgccgttgt ggatttgctg gtagcctgtt gaggttctct ctgagagcga ttccattatc
      481 tgtcccttta tatggcgctg cgttgaataa tggaatcagc tatacgccgg gcatctaccg
      541 gtaacaatcc agatgagcgt attacattgg ttttcttcag attatgcgag gcgatttcca
      601 gaaagaaagg agaatttcag cgccgactct tttctgagga ggcggcgctg ggacagagct
      661 taaacttcca gccagttcat aatgccgtct gccgctttgc gaccttcggc gatggcggta
      721 acgaccagat cggaaccacg gacgatatcg ccgccagcaa agattttcgg gttgctggtc
      781 tggaaagcgt tgtcgttgcc ttccggagcg atgatgcggc cttgtgaatc cagctcgacg
      841 ctgtgttttg ccagccattc catgttgtgt ggacggaaac caaacgccat gatcaccgca
      901 tctgccggaa cgatatgttc ggaacctgca acgatctccg cgcggcgacg gcctttggca
      961 tccggttcgc ccatttcggt acgtaccatt tttacgccgc tgactttgcc gttaccgttc
     1021 acttcaatcc ccagtggctg gacgttgaat ttgaactcta cgccttcttc ccgcgcgttt
     1081 ttcacttcgc ggcgggaacc cggcatgttc tcttcatcac gacgataggc acaggtaacg
     1141 tgctttgccc cctggcgtac agacgtgcgc acgcagtcca tcgcagtgtc gccaccgcca
     1201 agtactacca cgcgtttgcc ttccatgctg acgaacggtt cgtcgcgggt ttcaccaaag
     1261 cccattaact gtttggtgtt ggcgataagg aacggcagcg ctgcgtacac gccatcggcg
     1321 tcttcgtttt ccagcccgcc gcgcattgac tgataagtcc caacgccaag gaacacggca
     1381 tcgtaatcac tcagcagatc gtccagctgt acgtcgcggc ccacttcggt attgagtttg
     1441 aattcaatac ccatgccggt gaagatttca cggcgacgcg tcattacctc tttttccagc
     1501 ttgaaggccg gaataccgaa ggtcagcagc ccgccaattt ctggatgacg gtcgaagaca
     1561 acggctttta cgccgttacg cgtcaggaca tccgcacacg ccagacctgc cgggcctgca
     1621 ccgataatcg ccactttttt accggtttgc ttcacgccgg acatatccgg acgccagccc
     1681 atttcgaacg ctttatcatt gatataacgc tcaatgttgc cgatggtcac cgcaccgaac
     1741 tcatcgttca gggtgcagga accttcgcac agacggtctt gcgggcagac acgtccgcat
     1801 acttccggca gtgtgttggt ctgatgcgac agttctgccg cttcaaaaat acgcccctcg
     1861 ttggcgagct tcagccagtt tgggatgtag ttgtgtaccg ggcatttcca ctcgcagtat
     1921 gggttgccgc acgacagaca gcggtcagcc tgcgctttgg cctggccttc ggaaaacggc
     1981 tcgtaaattt caacaaactc aattttacgg atcttcagcg gtttctttgg cggatcaacg
     2041 cgctgcaggt cgataaattg ataaacattc tgactcattg ttgctacccc ttactgcgcc
     2101 tgcacgcgca actctgctgc gctacgacta cggtgaccca gcagtgcttt tacatcactg
     2161 gacttcggtt taaccagcgc gaatttcgtg gcgaaggttg accagttcgc cagaatctct
     2221 tcaccgcgct gagaaccggt atgctgaaca tgctcggtga taagaccgcg cagatgctct
     2281 tcatggatcg ccagatcgtc gacgcttaag acttcgacca gttccgggtt aacgcgtttg
     2341 cggaaatcac cgctttcatc gagaacgtaa gcgaagccgc cggtcatgcc cgcgccgaag
     2401 ttaacgccgg ttttacccag aatacagaca ataccgcccg tcatatattc acagccgttg
     2461 tcgccaatgc cttctaccac ggtgatagca ccggagttcc gcacgccgaa acgttcaccc
     2521 gcgcggcctg cggcatacag acgaccaccg gtcgcgccat acaggcaggt gttgccgata
     2581 atgcttgctt catggctgcg gaaggcggaa ccaaccggag gacgaatggc gattaagccg
     2641 cccgccatgc ctttaccgac atagtcgttg gcatccccgg tcaggtacag ttccacgccg
     2701 cccgcgttcc acacaccgaa actctggcct gcggtgccgt tgaagtacgc tttgatagga
     2761 tcggctgcca gcccctgatc gccgtgcgtc tgggcgatat agcctgaaag cgacgcgccg
     2821 acagaacggt cggtgttgcg gatatcgaac cagaaggttt tgctctggcg ttcatcgaca
     2881 aacggtttcg cctgttgcag caactgcgcg ttcagtaggc cgttatcaaa cggcgggttg
     2941 ttttcagtgc agtagagtga cttacctgca tgtggctcgg cagtctccag cagcttcgac
     3001 agcgccagtt tctgctgttt ggcggtgaaa ccgtccagct ctttcagcag gtcggtgcga
     3061 ccaatcagat ccaccagacg cgttactccc agctgtgcca tcagctcgcg ggtttcacgg
     3121 gcgataaact caaagtaatt agtcaccttg aacggcaggc cgtgatagtg attcttacgc
     3181 agtttgtcat cctgagttgc tacacccgtg gcgcagttgt tcaggtggca aatacgcaga
     3241 tatttacagc cgagcgccac cattgggcca gtgccgaagc cgaagctttc tgcgccgaga
     3301 atcgccgctt tgatgatatc gacacccgtt ttcaggccgc catcaacctg caaacgaatt
     3361 ttatgacgca agccgttggc aaccagcgcc tgttgggttt ccaccagacc gagttcccac
     3421 gggcagcctg catatttcac tgacgacagc gggcttgcgc cggtgccgcc gtcatagccc
     3481 gcgatggtga tcaagtccgc ataagctttc gccacgccag tcgcgatggt gccaacgcct
     3541 ggttcggaca ccagcttaac ggagatcatc gctttcgggt taacctgctt gaggtcgaaa
     3601 ataagctgcg ctaagtcttc gatagagtag atatcgtggt gcggcggcgg ggagatcagc
     3661 gtcactccgg gcaccgaata gcgcagtttg gcgatgtaag gagtgacttt gtcgcccggc
     3721 aactgaccac cttcgcccgg cttcgcgccc tgggcgactt taatctgaat gacgtcggca
     3781 ttgaccagat acgccggagt aacgccgaag cgaccagaag ccacctgctt gatgcgcgac
     3841 actttgttag tgccatagcg cgccgggtct tcgccgcctt caccggagtt cgagttaccg
     3901 ccgatgctat tcatcgcttc cgccagcgcc tcgtgggctt ccgggcttaa cgcgccgata
     3961 gacatcgcgg cggtatcaaa gcgtttaaac agttcgcttg ccggttcaac atcagcaatg
     4021 ttgaccgcgt tttcacccgg cgtaattgcc agcagatcgc gcagcgtggt tgccggacgc
     4081 tcattaacca gcttcgcgta ttcctgatag tcgctgtact cgccgctttg taccgcctgt
     4141 tgcagcgtgc gcaccacgtc cgggttgtag gcgtggtatt cgccgccgtg gacgtatttc
     4201 agcaggccgc cctggctaat cggcttgcgc gccagccagg cacgtttcga cagattcagc
     4261 agatcctgct ggaagtcttc aaagcttgcc ccaccaatgc ggctgaccgc gccctggaag
     4321 cacaggccca ctacatcatc gtgtagaccg accgcttcaa acagtttcga gcagcggtaa
     4381 gaggcgatgg tggagatgcc cattttggac atgattttgt acaagccttt gttgatgccg
     4441 ttacggtagt tgagcatcac ggtacgataa tctttggcaa tcgcgtgggt gtctaccagg
     4501 cggcccagcg tttcataggc aaggtacgga taaatagccg tcgcgccgaa acccagcaac
     4561 acggcgaagt ggtgcggatc gcgggcgctg gcggtttcga cgatgatgtt ggcatcgcaa
     4621 cgcaggcttt gatcgaccag acgggtctgg atcgcgccaa ccgccatcgg agccggaacc
     4681 ggcaggcgat ctttagcgat attccggtcg gagagcacca gcaacacggt gccgctacgt
     4741 accatttttt cggctttgtc gcatagctct ttgactgtcg cttcgagcgt ggttttagtg
     4801 acgtcaaagg tgatatccag cgtatctgcg cggtagtgct cctctttcat cgtcgtgagc
     4861 tgtttgaaat cggagtagag cagaatcggc gatttaaagc ttaaacggtg cgcctggccc
     4921 tctgcttcgc aaaagacgtt catttcacga ccgatactgg tggcgagcga cataacatgc
     4981 gcttcacgca gcgggtcgat tggcgggtta gtcacctggg caaactgctg gcggaagtag
     5041 tcgtaaataa tgcgcggctg actggagagc acggcgaatg gggtatcatc gcccatcgaa
     5101 ccgaccgctt cctgaccgtt ttcgcccagt acgcgaatta cggagtccag ctcttccgcg
     5161 ctgtagttaa actgtttctg gtagctggca agcgtgtcgt cgtccagttc gcggctaccc
     5221 acttcttcat cgggcagatc ttcaaacggt accagtcggc ggacgttttt ctccatccac
     5281 tctttataag ggtggcggct tttcagatcg tcatcggttt ctgccgagtg cagaatacgc
     5341 ccactgcgag tgtcgatcac catcagttcg cctggcccga cgcggccttt ttcgaccact
     5401 tcgtcaggct ggtaatccca gataccgact tcagaggcgc aggtgatgag cttgtctttg
     5461 gtaatgacgt agcgtgccgg acgcagaccg ttacggtcga ggttacaggc agcgaagcga
     5521 ccatcggaca tcacgatgcc cgccggacca tcccacggct ccatatgcat ggagttaaag
     5581 tcaaagaagg cacgcagttc cgggtccata tccgggttgt tctgccaggc gggtggcact
     5641 aacaggcgca tggcgcggat gatatccatc ccgcctgcca gcagcagttc cagcatgtta
     5701 tccatggaac tggagtcaga accggtttcg ttgacgaatg gcgcggcgtc gtgcaggtca
     5761 gggatcagcg gcgtctggaa tttataggta cgcgcacgcg cccattggcg gttaccggtg
     5821 atggtgttga tttcaccgtt atgcgccaga tagcggaacg gttgcgccag cggccagcgc
     5881 ggtacggtgt tagtggagaa gcgctggtgg aacaggcaaa tggccgattc cagacgcagg
     5941 tccgcaagat ccagataaaa gcgcggcaga tccgccggca tacacagacc tttatagatg
     6001 ttcaccagat tcgacaggct acagacgtag aagtctttgt cggcttcgag acgcttttca
     6061 atgcggcggc gggcgataaa cagacggcgc tccatatcgc gtggacgcca gcctgccggg
     6121 gcgttcacaa aaatttgctc aatgcgtggc agagaggaga gggcgatttc acccagcacg
     6181 ccttcgttag tggggacatc acgccagccc acaatcgaca aggtttcgcg ttgcagttct
     6241 tcttcaacga tgcggcgtgc ggcagcggcg agttcaggat ctttattcag gaagagcatc
     6301 ccgacagcgt agttttttgc taaacgccag ccgcgctcct gcgcaacgat gcgaaaaaag
     6361 cgatccggtt tttgtaacag caagccgcaa ccgtcgccgg ttttaccatc ggcgagaatc
     6421 gcgccacggt gctgcatgcg ggccagtgcg tgtattgcag tacgcactac cttgtggcta
     6481 ggttcgcctt ctatgtgggc gatcaggccg aaaccacagt tatccctctc aagggattta
     6541 tcgtacaaca tatcgtgaac ctcccccagg ctctgcggga accccacttc cgaaccgctg
     6601 cgcacgggca cagaaagagc gtggcgacgg ggtttgcgtg tcatacgcgc gcctcgcatt
     6661 cgcccccttg ttgtcctttt cgcatcggtt aatacggtga agaggggatc tcaattactg
     6721 cataaatatg atgagcaggc tgctcatcca gaaagcttcc agcggatttc caacttatcg
     6781 ggaaagctga tacaggtcaa atggcaagct tattggtaca gaaatgtgct ttagcgcaaa
     6841 attctttatt aaatagctga aattaaaata gattttatct ttaaggctaa aaggagagtc
     6901 gttagagaaa cagtcaattc ctgactgtca taaaggcgtg ttaaccctta tcctgccttg
     6961 aactgacgag aaaaccagca ttttatactg ccttaattgg taatgacgtt tcatgctact
     7021 gtttttgcct aaaatccata aggagataaa aaaatctgac acaagataag caaaagtgat
     7081 ttatgtgatt gtgcatgaaa tttcggtaat tttgattgat tatgcagttg ataaaagcag
     7141 gccgggacga ttgatccagg tcatcgccga attgagctaa aaatggcagg ctttggcctc
     7201 ttttcaaggc gcggtctatc agattatgca gttacagaaa ttagtcaata tgtttggtgg
     7261 tgatctcacc cgtcgttatg ggcaaaaggt gcataagctg acgctgcacg gcggttttag
     7321 ctgccctaac cgtgacggta ccatcgggcg tggtggctgc acattctgta atgttgcctc
     7381 gtttgccgat gaagcgcagc agcatcgttc cattgccgag caactggcgc accaggcgaa
     7441 tttagttaac cgcgctaaac gctatctggc ctactttcag gcgtatacca gcacctttgc
     7501 ggaagttcag gtgctgcgtt ctatgtatca gcaggcggtg agccaggcca atattgtcgg
     7561 tttgtgtgtt ggtacccgcc cggactgcgt gccggatgcg gtgctggatc tgctttgcga
     7621 atataaggac cagggttacg aagtgtggct ggagctgggg ctacaaaccg cccacgacaa
     7681 aacactgcat cgcatcaacc gcggccatga ttttgcctgt tatcagcgta caacccagct
     7741 ggcacgtcag cgcgggctga aggtgtgttc ccacttaatt gtcggcctgc ctggcgaagg
     7801 gcaggctgaa tgtttgcaaa cgctggagcg agtggttgaa accagcgtgg acggcataaa
     7861 gctgcatccg ctgcatattg tgaaaggcag cattatggcg aaagcctggg aagcggggcg
     7921 tttgaacggt attgaactgg aggattatac gctcactgcc ggagaaatga ttcgccatac
     7981 gccaccggaa gtgatctacc accgcatttc cgccagcgcc cgtcgcccga cgctgcttgc
     8041 tccgctgtgg tgcgaaaatc gatggacggg aatggtcgag ctggatcgct atctgaatga
     8101 acatggcgta cagggatcgg cgctgggacg tccgtggcta cctccaacgg agtaggtcgt
     8161 tgaggggaat tccgcatttc tcacacaatt tataacgtaa ctgtcagaat tgggtattat
     8221 tggggcaggt tgtcgtgaag gaattcccta atgaagcaaa ttcgtctgct ggcgcagtat
     8281 tatgttgacc tgatgatgaa gttaggtctg gtgcgcttct caatgttgct ggcgctggcc
     8341 ctcgtcgttc ttgccattgt ggtacaaatg gcggtaacca tggtgctgca tggtcaggtc
     8401 gaaagcattg atgttattcg ttctatcttc tttggtttgc tgattacgcc gtgggcggtc
     8461 tactttctat cggtggtcgt cgagcaactg gaggagtcac gacaacgtct gtcacggctg
     8521 gtgcaaaaac tggaggagat gcgcgagcgc gatttgagcc tcaacgttca gttaaaagat
     8581 aatattgccc agctaaatca ggaaattgcc gttcgtgaaa aagcggaagc agaactgcag
     8641 gaaaccttcg gccaactgaa aattgaaatc aaagagcgcg aagagacaca aattcagctc
     8701 gagcagcaat cctcattctt acgttccttc cttgatgctt cacccgactt ggttttttat
     8761 cgtaacgaag ataaagagtt ttccggctgt aaccgcgcga tggagctgct gaccggaaaa
     8821 agcgaaaaac aactggttca cctgaaacct gctgatgttt actcaccgga agccgccgca
     8881 aaagtcattg aaaccgatga aaaagtgttc cgtcataatg tgtcactgac ctatgaacag
     8941 tggctggatt acccggacgg gcgcaaagcc tgctttgaaa tccgtaaagt gccgtactac
     9001 gaccgcgtgg gtaaacgtca cggtttgatg ggctttggtc gcgacattac cgagcgtaag
     9061 cggtatcagg atgcgcttga acgcgccagc cgcgacaaaa cgacgtttat ttccaccatc
     9121 agtcacgaat tgcgtacacc gctgaacggt atcgtcggcc tgagccgcat tctgctggat
     9181 accgaactca ccgccgagca ggaaaaatat ctcaaaacta tccatgtttc ggccgtcacg
     9241 ctggggaata tctttaacga tattatcgac atggataaga tggaacggcg caaggtccag
     9301 cttgataatc agccggttga tttcaccagc ttccttgccg atctggaaaa tctctccgca
     9361 ttgcaggcgc aacaaaaagg attgcgcttt aacctggagc ctacgctgcc attaccgcat
     9421 caggtcatta ccgacgggac gcgtttacgg cagatcctgt ggaacctcat cagtaacgcc
     9481 gtcaaattca cccagcaagg ccaggttacc gtgcgcgtgc gctacgatga aggcgatatg
     9541 ctgcattttg aagtggaaga ttctggtatc ggcattccgc aggatgagct ggataaaatc
     9601 ttcgccatgt attaccaggt gaaagacagt catggcggta aacctgccac cggcaccggt
     9661 attggtctgg ccgtttctcg tcgtctggcg aaaaatatgg gcggcgatat tacggttacc
     9721 agtgaacagg gcaaaggttc aacctttacg ttgacgatcc acgcaccgtc ggtggcagaa
     9781 gaggtcgatg atgcgtttga tgaagacgat atgcctttac cggcgctgaa tgtgctgctg
     9841 gtggaagaca ttgaactgaa cgtgattgtc gcgcgttctg tgctggaaaa attaggtaac
     9901 agcgttgatg tcgccatgac cggcaaggcg gcgctggaga tgtttaaacc gggcgaatac
     9961 gacctggtgt tgctggatat tcagttgcca gatatgaccg ggctggatat ctctcgtgaa
    10021 ctgacgaagc gttatccgcg cgaggattta ccgccgctgg tggccttaac cgctaacgtg
    10081 ctgaaagaca aacaagagta cctcaatgct ggaatggatg atgtgctgag taagccgctt
    10141 tcggttccgg cgctaaccgc gatgatcaag aaattctggg atacccagga tgatgaggag
    10201 agtacggtga cgacagaaga gaacagtaaa tcagaagcat tgctcgatat tcccatgctg
    10261 gaacagtatc tcgaacttgt aggaccgaag ctgatcaccg acgggttagc ggtgtttgag
    10321 aagatgatgc cgggctatgt cagcgtgctg gagtcgaatc tgacggcgca ggataaaaaa
    10381 ggcattgttg aggaaggaca taaaattaaa ggtgcggcgg ggtcagtggg gttacgccat
    10441 ctgcaacagc tgggtcagca aattcagtct cctgaccttc ccgcctggga agataacgtc
    10501 ggtgaatgga ttgaagagat gaaagaagag tggcgtcacg acgtagaagt gctgaaagcg
    10561 tgggtggcaa aagccactaa aaaatgaccc cggctagacc ggggtgcgcg aatactgcgc
    10621 caacaccagg gaaatcttgg ctgcgccgta aattattatg atgagttaca agggcacagc
    10681 actgtttttc aggccgcacg cagttaagat agcaaaactt aaatgatttg ttacatgaat
    10741 cagttaaatg tgtgatgcgt accattttta ttaaaaaaat taatgtgttt cagtcaattg
    10801 cgaaaggatg atcacaatga agaaaattgg cgtaattctg agcggatgcg gcgtctatga
    10861 cggttctgaa attcatgaag cggtgttgac gttgttagct atttcacgca gcggtgcgca
    10921 ggcggtctgc tttgcaccgg ataagcagca ggttgatgtt atcaaccatt taactggcga
    10981 agcgatgacg gaaacgcgca atgtgctgat tgaagcggca cgaataacgc gcggtgaaat
    11041 ccgtcctctg gcccaggccg atgccgctga actggatgcg ttgattgtgc cgggggggtt
    11101 tggcgcggcg aagaatttaa gcaattttgc cagtcttggt agcgaatgca ccgttgaccg
    11161 tgaattaaag gcgctggcac aagcgatgca tcaggccgga aaaccgcttg gttttatgtg
    11221 tattgccccg gcgatgctgc cgaaaatttt cgatttcccg ctgcgtttga ccatcggtac
    11281 tgatatcgat accgcagaag tgctggaaga gatgggcgcg gagcatgtgc cgtgtcctgt
    11341 cgatgatatc gtggttgatg aagacaataa gattgtcacc accccagcat atatgctggc
    11401 gcagaacatt gcagaagcgg cgagcggcat tgataagctg gtttcccgcg tgctggttct
    11461 ggctgaatga gtaaaagccg cttaacggtg tttagtttcg ttcgccgttt tcttttgcgg
    11521 ttaatggttg tcctcgccgt tttctggggc gggggcatcg cgttgtttag cgttgcgcct
    11581 gttcccttct cagcggtaat ggtcgagcga caggtcagcg cctggctgca tggcaatttt
    11641 cgttacgtgg cacattctga ctgggtcagt atggatcaaa tctcgccgtg gatgggactg
    11701 gcggtgattg ccgcagaaga tcagaaattt cctgagcact ggggctttga tgtcgcttcc
    11761 attgagaaag ccctggcgca caacgagcgc aatgaaaacc gtattcgcgg tgcttcaacg
    11821 atttctcagc agacggccaa aatctctttt tatgggatgg gcgtagctgg gtacgaaaag
    11881 ggctggaagc tggattaacg ctggggatag aaacagtctg gagcaaaaag cgtatcctga
    11941 cggtttacct gaatatcgcc gaatttggcg acggtgtgtt tggcgtcgaa gctgcggcac
    12001 aacgttattt ccacaaaccc gcgagcaaac ttacccggtc ggaagctgca ttactggcag
    12061 ctgtattacc taatccactt cgtttcaaag tctcctcgcc atcgggctac gtgcgtagcc
    12121 gtcaggcgtg gattttacgg cagatgtatc agttaggcgg tgagccgttt atgcaacaac
    12181 accagctgga ttaagcttcg cgcgaagtga gggccagcgt tttgctctca cgaggttgca
    12241 gatctttctg cagggcaata gccagcgccg gttgggcgat aaatcgtttc cataaccgct
    12301 cttgcttacg cagacaacac cacttcgacc aggtcgccag tggaatcaat gtgctttcac
    12361 caatgttgtc gcagacgacc gggataacct ctgactcact gatgcggtga cagaggatat
    12421 tctgcggctt cagcgacatc gtcacgatac ggttatcctg caaataacgc ttcagctgtt
    12481 tcagtagctg gcgcagttgt gcaatgtctt cttcgtaacg gcattgttca gcaaattcgg
    12541 tcagcgtaat ggaaggcttg ccgtcaaaat cggcaatcac atcgtagaca taaccggttc
    12601 cgcaatccgt ctcaacggta ccgtgatagc gcggtattcc actccagtct tttaaccggc
    12661 gaccaagatg cgcgtagtac tttaactcac ggcggatctc tttatcgcca ccatcgccac
    12721 gatggtagac aatcttgata cagcgttggg catcttccgg atgcgcataa catttccgat
    12781 gtcttccggt gcccagggga ctttgttcag ataagcgaat catgccatct cctggaaata
    12841 ttttctgacg atctccagta tggtcgatta acttaaacaa aacctaaaca atgcctctta
    12901 aggttttctt aaggttcttc tgaaagtgaa aagcgcacaa aatgtacaaa tgacgttgat
    12961 tgagaacgat ttcagtacgt gattttctta ttgtcggggg agcttgaagg gagttgtatg
    13021 tcaaagtgat gaagattaat cttcatcaaa accagaatta aagagggcga taacggcggc
    13081 cagtgcttcc tcttcctgtg gaccggtcgc ttcaacttca atctgccgtc ctttggcaga
    13141 atccaacatc agcagcgcaa taacgctgtt ggcttcagcc tcggtgcctt cgtcattacg
    13201 taacagcact tcagcgtcaa aaccctgcat taattcaaac agcttcattg caggccgggc
    13261 atgcatgccc agcttgtttg tgatttcaac agtttgcttg acggtcatgg tttacgtttt
    13321 tccagcgtac gatggcgtga ctggacgttt ttaccgcgcg agcggaagta gtctgccagt
    13381 tgctctgcaa tatacaccga acggtgcttc ccgccggtac aaccaatggc gaccgtcagg
    13441 tagctacggt tgttggtttc cagcataggt aaccatagct caagatagct tcgcgtctgg
    13501 tagataaaat tgtgtacttc tgtgtggcgg tcgaggaacg cggcgacagg tttatcaaga
    13561 cctgtcattg gacgcagttt cggatcccag tgcgggttcg gcaagaagcg cacgtcaaag
    13621 acgtaatctg catcgatagg gataccgtgt ttgaagccga aagactcaaa gaccatggtc
    13681 agttcgcgtt cacgtttacc cagcagacgg gtacgcagca tttcagccag ctcgtgaacg
    13741 gacatttctg aggtatcgac aatcagatcc gctcgcgaac gcagaggctc cagcaaatcg
    13801 ctttctttgt cgatagcact ttccagcgac aggtttttgc tggaaagcgg atgcagtcgg
    13861 cgcgtgtcac tgtaacgacg aattaaggta ttacggtcgg catccaggaa cagtagttgc
    13921 ggtgagaaag cgtcaggcag gttactcatc gcctgttcga atatttctgg tgactccggc
    13981 atattacgaa catcaatgct gacggcggca gaaatctcgc gatcggccag agttcgagcc
    14041 agatcgggta acaacactac gggaaggtta tccacgcagt aaaaacccat atcttccagc
    14101 gcacgcaggg cgacagattt acctgaacct gaacgtccgc tgacgatcat cagtaccatg
    14161 tactgtttct cctcacaacg tctaaaagag acattaccga ataactacgc ttcatccgga
    14221 gtaccttcgg tatccgtaat gatttgatac agctcttcat cgctctgggc tgcgcgtaaa
    14281 cggcggcaga tggttttgtc cgccagacgt ttcgccacca gcgacagagt atgcaggtgc
    14341 gttttagttt ggtctgccgg caccagcagg gcaaaaagta agtccaccgg ttggttgtcg
    14401 atggcatcga aagctatagg cgtttcgagc tgaacgaaaa cgccaacggc acgcagagta
    14461 tcttcttcaa gtttgccatg cggaatggca ataccattgc cgataccggt actgcccatt
    14521 ttttcccgcg tcaggatagc ttcaaaaacc acctgaggcg gcaggctaag ttgtttcgcc
    14581 gccagctcgc tgatgatttc cagggcgcgt tttttgctct gacagtggac gcggcttcgc
    14641 gtacattccc tgttaagaac actgctaagc tgtagagttg tatcattatt tgtcataatt
    14701 tcacctaaga acctgcccac tcaaacagtg gaacaggctc ggagcattaa ccgtacaaat
    14761 ggcccgttgt gccgcacaac aggccgccat gcacatgcta attgcccgga caattagtgt
    14821 tgtttcagtt tatctttatg tttggtcaac tggcgtgcca gcttatcaat taagccatca
    14881 atggcagcgt acatgtcctg accttccgcg ctggcatgaa tttcaccacc gtttacatgc
    14941 agtgttgcat ctgaggtgtg ggtgactttc tccactttca gaacaacata gacctggttg
    15001 attcggtcaa aatattgctc aagtttggca aatttagctg taacaaattc gcgcagtgcc
    15061 tcggtgatct cgacgttatt tccggtaatg ttgagctgca tagtgtcttc cttatcggtt
    15121 gggtcaaacg agctgtttac gctggtttga cggcggaatg gataaagact ctcggtactt
    15181 cgcaacagtg cggcgtgcca ccatgatacc ttgttccgac agcaaagagg ttaacttgct
    15241 gtcgctcaac ggtttcgctg ggttttccgc cgcgattaat ttcttcacca gtgcacgaat
    15301 cgccgtggag gaagcttcgc cgccgccctc ggtattgacg tgactggaaa agaaatactt
    15361 cagttcaaaa atgcctcgtg gactatgcag gtatttttgc gtggtcacgc gagatatcgt
    15421 cgattcatgc atttcgacag cctgggcgat atcggccagt accatcggtt tcatatattc
    15481 ttcaccttgc tcaaagaagg cttgctgctg ttcaacgata cagcgactca cgcgcagtag
    15541 cgtatcgtta cggctttcca gactcttgat caaccatttg gcatcctgca gattgctgcg
    15601 gataaactgg ctgtcaccat cgttgcgcgc gttattgcac atcgaggcgt agtgctggtt
    15661 gatttgcaga cgcggaatgc tgtcactgtt gagttctacc gtccagtgac cgttatgctt
    15721 acgcaccagc acatctggaa tgacatactc aggttcgcca gtctggatcg actgcccggg
    15781 gcgcggatcg agcgactgga tcagattgac ggcttctttc agcacatctt ctttcagacg
    15841 cgtgacgcgc attaaagtgc ggaagtcgtg attggctaac agatcgagat gatcgctaat
    15901 gatcaatctg gcctcttcca gccacggcgt ggtcttatcg aattgggaga gttggattag
    15961 caggcagtca cgcagatctt ttgccgctac accgaccggg tcaaaccgtt ggatccgctt
    16021 aaggacggct tcaacctcgt cgatgtcgat ctcttcatcg cccatacttt cgagaatatc
    16081 ttccagcggg acagtcagat aaccggtttc atcaacggca tcgacgatag aggtagcaat
    16141 agcgcggtca gtgtcggaaa acggtgtcag ctcgacctgc cacatcaggt aatcctgcaa
    16201 ggtctgcgtc gtttcgccct ggtagaccgg cagctcgtcg tcaatgtagt caccgctggt
    16261 gccggatggt gtaccagcgg tgtaaatggt gtcccaactg gcatcgagcg gcagctcttc
    16321 cggcatctct ttttgttcga gcgcgtcggc ggtgtccagc gtttcactgt cttgcgtttc
    16381 gcgggtgtcg atttcttcat gagtgtcgat ttgctcaagc agcggattac tctccagcgc
    16441 ctgctgtagc tcctgctgaa gttccagcgt cgacaactgc aacagacgaa ttgcctgttg
    16501 gagctgtggc gtcatcgcca gttgttggct aagcctgagt tgcaaacctt gcttcatgtt
    16561 cagaatcgta ctctcctgct aaaacgtcgc aaacttctac cctatcagag tctgaagtct
    16621 tccccaaggt atacacgctt aacgtgttcg tcttgtaaga tttctgtagg cgtgccgtgg
    16681 gcgatcaaat gcccctgact gacgatataa gcgcgttcac aaaccgccag tgtttcacgc
    16741 acgttgtggt cagtgatcag cacgcccagg ccgctgtcgc gcaggtgttc aatgatgcgt
    16801 ttaatgtcga taaccgagat cgggtcaacc ccggcaaacg gttcgtcgag cagaataaat
    16861 ttcggattcg cagccagtgc gcgggcaatt tctacacggc gacgttcacc cccggagagt
    16921 gactgcccca tgctgtcacg caggtgctca atgtgaaact cttccatcag ctcgttcgcg
    16981 cggtcttcac gttgttcagc agacaagtcg tcacgaattt gcagtaccgc catcaggtta
    17041 tcgtaaacgc tgaggcgacg gaaaatggag gcttcctgtg gcagatagcc gataccgcgg
    17101 cgcgcgcgtg catgcagagg cagcagactg atatcgtcat catcaataat gatgttgccc
    17161 gcatcgcgcg gcacaatgcc tacaaccatg tagaaagtgg tggtcttacc ggcaccgttt
    17221 ggccccagca gaccgacaat ttccccggag ttgacggtca ggctgacgtc ttctaccacg
    17281 cgacggcctt tataggcttt tgcaaggttc tttgcagtta atgttgccat aacgaattaa
    17341 ttacccttct tctgtgccgg ggtctggcct ttgttgtttt tgtcctgcag ctgcgacggc
    17401 accagaacgg tggttacgcg cttgcctttg tcgctgaaag cctgcatttt ctgctctttc
    17461 accaggtaag tgatcttatc gcccttaatg ttgctatcga cctgctgcag ataagcatta
    17521 cccgtcagaa cgacaaaatc ttttgccagt tcgtagtgca tctgggaagc gtgaccttca
    17581 acgggtttac cgttgtcctg catctggtag aacgttgccg gtttaccgta tccgtcaatc
    17641 acttctttac cttgttcgcc gcccggacgg gtaacgacca ctttgtcggc attaattttg
    17701 atggtgccct gggtgacgat gacattaccg gtaaaggtaa ccacgttgcc ttgcatatca
    17761 agagattgct ggtccgattc aatgtggatc ggctgatcag tgtctccggt tacggcaaat
    17821 gccggaatgc tggcggccag aagtgagctg gcaagcacaa gattaaggct gagtttgttt
    17881 gttttgaatt tcataggatg ttctaacctt ttcaatcagc tcggcgttct tgctgcgtaa
    17941 gttgccgcgc attttcagac cgctggagtt aaatgttgtt ccgtataacg tgacgaggtc
    18001 ttcagaggta acatcctgcg tcaccagatt gatctgcgcg ttatccgtcg tgattctgcg
    18061 aagttgagag tctggcacaa gtgcgttgac ttcaacgtgt ccatataaat agagcatccg
    18121 gtcattggtc agcttggctt tatctgcttt tacggaccat gtcgggattt tatccttatc
    18181 aaacgtggta agtaccggct gcgtaaacca cgaaacggcc tgatcggaat aatattcaac
    18241 gtgttgagca atcaatcgat agcttagtgc cccttctggg ttatagacga gcgtgtccgt
    18301 atgctcgctt ttataggtgg gatcattgtt gttgacgacc acctgggcgg tatcgtcttt
    18361 ttcggccata ttaatgccga tcatcaccag aaccgccagt gatagcacaa tgataaccca
    18421 acgtctggct ttactcatat cgattgccct ttggcttcat ccagtttgcc ctgcgccagg
    18481 agtaataagt cgcaaacttc gcgcactgcg ccacgaccgc cagcaatgcg cgtcacgtaa
    18541 tcggcgcgcg ggatcaacag tggatgcgca tcggccacgg cgacgcttaa acccactttt
    18601 tccattaccg gccagtcgat gagatcatcg ccaacataag ccacattttc cggggcaatc
    18661 gccaattttt ccagcaaatc gctaaaggcg atcagtttgt ttgactgccc ctgatacaag
    18721 tgagtgatcc ccaatgtggc acaacgatct tctaccagtt tagcctttcg cccggtaatg
    18781 atagcgactt caatatcaga ggtgagcgca caacgaatgc cataaccgtc acgaacattg
    18841 aacgctttca gctcttcgcc gttattgccc atataaatca ggccatctga cagtacgcca
    18901 tcaacgtcga ggatcagcag acgaatgttc tctgcttttg ccataacgtc ggcgctgaca
    18961 gggccgtaac aggtcgcaag cgacgcacct gctttgctca ttgttgttta tccttgaatc
    19021 tttacactac gcctgcacgc agtaaatcat gcatatgtaa cacaccgagt aaatggtcgc
    19081 catcggcaac catcacggag gtgatatggc gggactgcat taagttcagt gcctcaacgg
    19141 ccagaatgcc agggcgcaca cgtattcccc ccggcgtcat cacatcggca atacttaact
    19201 gacgaacatc cacgcccata tcgaagacac ggcgtaaatc accgtcggta aagatgcctt
    19261 caatcatcat attgtcatcg caaatgacag tcataccaag atttttgcgg gtaacttcca
    19321 gcaatgcgtc acgcagactg gccgttttct taacatgcgg gatctcatcg cccgtatgca
    19381 taatatcgtt tacgcgcagc agaagtttac gacccagtgc gccgcctggg tgtgagagcg
    19441 caaaatcttc agcagtaaag ccgcgtgctt ttaacagcgc gacagcgagg gcatcgccca
    19501 taaccagcgt ggcggtggtg ctgctggtcg gtgccagccc taacggacag gcttctttcg
    19561 ctactttaac acacagatgc acatctgcgg cgcgcgccat gctgctctcc gggcgaccgg
    19621 tgatgcagat taacggtacg tgaagacgct taagcactgg aattaaggcc gtgatttcgc
    19681 tggattcacc tgagttagag atagcaatca ccacatcctg tggggtgacc atgcctaaat
    19741 caccatgcgc ggcttcacta ggatggacga aaaatgaagg tgtaccggtg ctggcaaacg
    19801 ttgcggccat tttgcgcccg atgtgccccg atttccccat ccccatgacg acaactttcc
    19861 ctttacacca gaacatcttt tcacaggcaa gcgtgaaatt ctgattgatg tattgatcaa
    19921 gctccgccag gcattcacgt tcaatcgcca ggacttcttt acctgcttgc tgaaagtcaa
    19981 aacccggttg taactctacg tgcgacataa tgcgtttcca gttattcaac gagtattggc
    20041 gataaccagt acaacatcgc cagccatacg ataaatccgc cagttaataa tacccctaca
    20101 ccacggcccg gttgcgggga gcgccgccag cacagcaacg caaaaataat gctcaccagc
    20161 aacatcacgc tgtagtcacg actgtacgcc agtggatcaa tctctcctgg cgttatcagc
    20221 gcgggtaaac ccaacacgat gacaatatta aaaatgtttg cgccaatgat atttccgaca
    20281 gcaatgtcgt tttcaccttt gcgaaccccc gctattgcgg ttgccagttc cggcagactg
    20341 gttccgatag caattgccgt cagagccatc gtcaactcgc tgatggcaaa gtaattcgcc
    20401 agcaccgtgg cgttatcaac caccatccgc gtggccactg gcatgatgat aagcgcaatg
    20461 ccgagccata aaaacgcgac gggcaatccg ccgtcacgcg gcagctctgc aagctgctct
    20521 ctggtcaggc tgtcagtccc ctgacgtgca agtttaacaa tgaacagcag ccatagcaca
    20581 gccagaaaga ggagaaagat accatcgctt cgactaagtt gtccgtcata gagtacggaa
    20641 ccggccacca cgctgaccaa caacattaag ggtaattcac ggcgcagaac atcggaatgg
    20701 acggtaaaag gacgaaccag cgcagccaga ccgaggatca gcaatatatt gataatgttt
    20761 gagccgaggg ctgtaccaac ggctaaatcg cgttgttcgt gcagagacgc ggcaagcgag
    20821 acgatgattt ctggtaacga tgtaccaata ctgaccaccg tcatgccgat gatcagcggc
    20881 gggatgccaa aggttcggca aagaatagac gcggcaaaaa ccaggcggtc ggcactgtaa
    20941 acgaccaaaa gtaaaccaac aattaacagt gccgtagcta aaagcatcta acgtcctttc
    21001 ttcaggtata ctcgccggtc cgctgaagat tttcagaaag ccgtaacgga ttcttaattt
    21061 tgactttatg cggctaaaaa gtaaaacaaa tgccagcttt cgctaaccac ggcgggtaat
    21121 attctgtaaa tatgttgggt tcaaggttaa attgagcgcc atgcttagaa aatcaacgca
    21181 agacgaaggg tgaattatgg agcagtctgt ggcgaattta gtcgatatgc gcgatgtcag
    21241 ttttacgcgt ggcaatcgct gcatcttcga taatatttcc ctgaccgtgc cgcgagggaa
    21301 gatcacggcg atcatggggc catcgggcat cggtaaaacg acgctactcc gtctgattgg
    21361 cgggcaaatc gcaccagatc atggtgagat ccttttcgat ggtgagaata ttccggcgat
    21421 gtctcgttcg cgcctgtata cagtgcgtaa acggatgagc atgttatttc agtccggggc
    21481 gttgttcact gatatgaacg tatttgacaa cgtcgcctat ccactgcgcg aacataccca
    21541 acttcccgcg ccattgttgc atagtacggt gatgatgaag ctggaggccg tggggctgcg
    21601 tggagcggct aaactaatgc cttctgaact ttccggtggg atggcgcggc gtgcagcgct
    21661 ggcgcgtgcg attgcgctgg agccggatct catcatgttt gatgaacctt ttgttgggca
    21721 agatcccatc accatgggcg tactggtgaa gctgatttct gagctgaaca gcgcgctggg
    21781 cgtgacttgt gtggtggttt ctcacgatgt gccggaagtg ttaagtattg cggatcacgc
    21841 ctggatcctg gcggacaaaa aaattgtcgc tcatggcagt gcccaggcgt tgcagacgaa
    21901 tcctgatccg cgcgtacgtc agtttctgga cgggatagct gacgggcctg ttccgttccg
    21961 ctatcctgcc ggcgattatc acgctgatct tttaccaggg agttaagcca ctcatgctgt
    22021 taaatgcgct ggcgtcgctc ggacataaag ggattaaaac cctgagaacg ttcgggcggg
    22081 ccgggttaat gttattcaat gcgctggtcg gcaaaccgga atttcgcaaa catgcgccgc
    22141 tgctggtgcg ccagctctat aatgtcggcg tcctgtcgat gctgattatt gtagtttctg
    22201 gcgtgttcat cggaatggtg ttggggctgc aaggttatct ggttctgacc acttatagcg
    22261 cggaaaccag tctgggtatg ctggtggcgt tatcgctact gcgtgaactg gggccggtgg
    22321 ttgccgcgct gttgtttgcc gggcgtgctg gttcggcgtt aaccgcagaa atcggcctga
    22381 tgcgcgctac agagcaactc tccagtatgg agatgatggc ggtggatccg ctgcgtcggg
    22441 ttatttctcc ccgtttctgg gctggggtta tttcattacc actgttgacg gttatcttcg
    22501 tcgccgtggg gatctggggc ggatcgctgg tcggcgtcag ttggaaaggc attgatagcg
    22561 ggttcttctg gtcggcaatg caaaatgccg tcgactggcg tatggatctg gtcaactgtc
    22621 tgattaagag cgtggtgttc gccatcacgg tgacgtggat ttcgttgttt aacggctacg
    22681 acgccatccc gacgtctgcc gggattagcc gggcaaccac tcgcaccgtt gtccactcgt
    22741 ctctggctgt tctggggctg gattttgtgc tgaccgcatt gatgtttggg aattgagttc
    22801 atgcaaacga aaaaaaatga aatttgggtg ggtatctttt tattagcagc actgctggcg
    22861 gcgctgtttg tttgcctgaa ggcggcgaac gtgacgtcca tacgtactga atcgacctac
    22921 acgctttatg cgacgttcga taacattggc ggcctgaaag cgcgctctcc ggtcagcatt
    22981 ggtggtgttg ttgtgggccg ggtggcggat attacgctgg acccgaaaac ctatctgccg
    23041 cgcgtaacgc tggaaattga acaacgttat aaccacattc ctgataccag ttcgctgagc
    23101 attcgtactt ccggcctgct gggagaacaa tatctggcat taaacgtcgg ttttgaagac
    23161 ccggaactgg ggactgctat cctgaaggat ggcgatacaa ttcaggacac caagtctgcg
    23221 atggtgctgg aagatctcat tggtcagttc ctttacggta gtaaaggcga tgacaataag
    23281 aatagtggcg atgcgccagc tgctgcgcca ggtaataatg aaaccactga acctgtgggt
    23341 acaacgaaat aatttcagga gaaccgacgc atgtttaaac gtttaatgat ggtcgctttg
    23401 ctggtgattg cacctctgag tgcggcaacc gcggcagacc agaccaatcc ttataagctg
    23461 atggacgagg cggcgcagaa aacgttcgat cgcctgaaga atgagcaacc gcaaattcgg
    23521 gccaacccgg attatctgcg taccattgtt gatcaggaac tgctgccata cgtacaggtg
    23581 aaatacgccg gtgcgctggt gctgggccag tattacaaga gtgcgacccc tgctcaacgt
    23641 gaagcctact ttgccgcttt ccgtgagtac ctgaagcagg cttacggtca ggcgctggcg
    23701 atgtatcacg gtcaaaccta tcagattgcg ccagaacagc cgctgggcga taaaaccatt
    23761 gtgcctattc gcgttaccat tattgacccg aatggccgtc cgccggtgcg tctggacttc
    23821 cagtggcgta aaaactccca gacgggaaac tggcaagctt acgacatgat tgctgaaggc
    23881 gtcagtatga tcaccaccaa acaaaacgag tggggaacgc tgctgcgtac caaaggtatc
    23941 gacggcctga ctgcgcaact gaaatcgatt tctcaacaga aaatcactct ggaagagaaa
    24001 aaataatgag cgagtcactg agctggatgc agacgggtga cacgctggcg ttatccggag
    24061 agctggatca ggacgttctg ctaccgcttt gggaaatgcg tgaggaggcg gtgaagggga
    24121 ttacctgcat cgatcttagc cgtgtgtccc gcgtggatac ggggggactg gcactgctgc
    24181 tccatcttat cgatctggcg aaaaagcagg gcaacaacgt gacgcttcag ggggtaaacg
    24241 acaaagtgta taccctcgcg aaattgtata atttgcctgc tgatgttctg cctcgttaaa
    24301 ttttttcagg ccggatatgg cgttaaccgc ctgtccggca aacctgcctg caagtcgagc
    24361 cttgtctgat ttatcagcga ggctttttgc ttatttatgc cagcgcaact ttgctctaag
    24421 atgtttcgct ggtttattaa ctgatgattg aagatcccat ggaaaataat gaaattcaga
    24481 gcgtgttgat gaacgctctc tccctccagg aagtccacgt ttccggcgat ggcagccact
    24541 ttcaggttat tgccgtgggt gagttgtttg acggtatgag tcgggttaaa aaacagcaga
    24601 cggtctatgg cccgctgatg gaatatattg cggataaccg cattcatgct gtgtcgatca
    24661 aagcgtatac ccctgcggag tgggcgcgcg atcgcaaact gaacggcttt tgagctatgg
    24721 gcgattcgcc ggtagcggat atgaattgtt aactgagaac aaactaaatg gataaatttc
    24781 gtgttcaggg gccaacgaag ctccagggcg aagtcacaat ttccggcgct aaaaatgctg
    24841 ctctgcctat tctttttgcc gcactactgg cggaagaacc ggtagagatc cagaacgtcc
    24901 cgaaactgaa agacgtcgat acatcaatga aactgctaag ccagctgggt gcgaaagtag
    24961 aacgtaatgg ttctgtgcat attgatgccc gcgacgttaa tgtattctgc gcaccttacg
    25021 atctggttaa aaccatgcgt gcttctatct gggcgctggg gccgctggta gcgcgctttg
    25081 gtcaggggca agtttcacta cctggcggtt gtacgatcgg tgcgcgtccg gttgatctac
    25141 acatttctgg cctcgaacaa ttaggcgcga ccatcaaact ggaagaaggt tacgttaaag
    25201 cttccgtcga tggtcgtctg aaaggcgcac atatcgtgat ggataaagtc agcgttggcg
    25261 caacggtgac catcatgtgt gctgcaaccc tggcggaagg caccacgatt attgaaaacg
    25321 cagcgcgtga accggaaatc gtcgataccg cgaacttcct gattacgctg ggtgcgaaaa
    25381 ttagcggtca gggcaccgat cgtatcgtca tcgaaggtgt ggaacgttta ggcggcggtg
    25441 tctatcgcgt gctgccggat cgtatcgaaa ccggtacttt cctggtggcg gcggcgatct
    25501 ctcgtggcaa aattatctgc cgtaacgcgc agccagatac tctggacgcc gtgctggcga
    25561 aactgcgtga tgctggagcg gacatcgaag tcggcgaaga ctggattagc ctggatatgc
    25621 atggcaaacg tccgaaggct gttaacgtac gtaccgcgcc gcatccggca ttcccgaccg
    25681 atatgcaggc ccagttcacg ctgttgaacc tggtggctga agggaccggg ttcatcaccg
    25741 aaacggtctt tgaaaaccgc tttatgcatg tgccagagct gagccgtatg ggcgcgcacg
    25801 ccgaaatcga aagcaatacc gttatttgtc atggtgttga aaaactttct ggtgcacagg
    25861 ttatggcaac cgatctgcgt gcatcagcaa gcctggtgct ggctggctgt attgcggaag
    25921 ggacgacggt ggttgatcgt atttatcaca tcgatcgtgg ctacgaacgc attgaagaca
    25981 aactgcgcgc tttaggtgca aatattgagc gtgtgaaagg cgagtaatcg tctgagagct
    26041 ggcagccccg ttcgcggggc taccgccaga ccactcattt tttcggttta gtgtagctac
    26101 gcatcaactg cgttctgtcg ataaactcat gggtctgcgg atcatggtag cgtgatggcc
    26161 agataaccca ggggtcagtt cccagggctt tcgcaataat catctctcct ttcggccatg
    26221 ggcgcgataa tgcgtttgcc agcgttgagg aactcaaacc atttctgcga gattccgccg
    26281 ccattgacgt tcctttcttg cgcaaacctg caatgatgtc agcgggatgc cagtcaatga
    26341 aattactttc catctttcct cactccttaa gttagtcgct ttcattcaga acacttcttc
    26401 tgtcgggcat cactatactc ctgatggtct attgcgctaa aaagttcctg taaagagtgt
    26461 gaatattcag aatatataaa gattcatcgc ccttttttca ggtgttatag cgctgaatta
    26521 gtgtttttat ggaatttact ggaatcatcc cgcgcactgc gcgggatgaa gggaggggat
    26581 taacgatcgc gttgaacagc gatgtgcgcg aggccgatga gtgcttctcg ccaaggggtg
    26641 tccgggagca cctgtaacgc tgcgatggct ttgtctgctt cttcctcggc gcgctgacgc
    26701 gtccattcaa gagatccaca agcgttcatt gcttccagaa ccggttccag aagatggcga
    26761 ccgttacctt gttcgatggc ggtacggatc atctgtgcct gttctggtgt gccatgatgc
    26821 atcgcatgca gcagcggcag cgtcggttta ccttcgttca gatcgtcgcc gacgttttta
    26881 cctaactgtt cgccatcggc attgtaatcg agtaaatcgt cgatcaactg gaaagcagtg
    26941 ccgagatagc gcccataatc ctgcaggcct ttctcctcct ccggcgtaca gccagccaga
    27001 atcccggaac actgcgcggc agcctcaaac agacgcgcgg ttttgctata gataacgcgc
    27061 atgtagtttt cttcagtgat gtccggatcg ttaacgttca tcagttgcag aacttcacct
    27121 tctgcgatga cgtttacagc ttctgacatg acttccagca ctttgagtga accgaggctg
    27181 gtcatcatct ggaacgcgcg ggtataaata aaatcgccta ccagcacgct ggcggcattg
    27241 ccaaatgcgg cgttggcggt agctttaccc ctgcgcatat ctgattcatc cacaacgtcg
    27301 tcgtgtagca gagtcgccgt gtggataaac tcgatcaggg cagcaatggt gacatgcgca
    27361 tttccctcat agccaacagc tcgtgcagcc agtacagcaa tcatcggacg aatacgttta
    27421 ccgccgccgc tgacgatgta atagcctaac tgattgatca gttggacgtc ggaattaagc
    27481 tgctcaagga ttgccgcatt aacacccgcc atatcttgcg cggttaactc attgattttt
    27541 tctaaattca tcgcaaaagc cgggcttttc gccccgatta ccttactgtg aacaaaggca
    27601 acttagggtt tatggttagg aatatagggt aattgtactg aaaaaatggc acagataaac
    27661 gttaccgtac aagttgtgtt tttttcttcg tgtattgact gtagcacttg tcaaaggcgt
    27721 gcgttttgcg taatattcgc gccctattgt gaatatttat agcgcactct gaatcattga
    27781 aaaggtgtgc gcggaagcgg agttttatat gtacgcggtt ttccaaagtg gtggtaaaca
    27841 acaccgagta agcgaaggtc agaccgttcg cctggaaaag ctggacatcg caactggcga
    27901 aactgttgag ttcgctgaag tgctgatgat cgcaaacggt gaagaagtca aaatcggcgt
    27961 tcctttcgtt gatggcggcg taatcaaagc tgaagttgtt gctcacggtc gtggcgagaa
    28021 agttaaaatc gttaagtttc gtcgtcgtaa acactatcgt aagcagcagg gccatcgtca
    28081 gtggttcact gatgtgaaaa ttactggcat cagcgcctaa gacctgagga gagatttcaa
    28141 atggcacata aaaaggctgg cggctccaca cgtaacggtc gcgattcaga agctaaacgc
    28201 ttgggcgtta agcgtttcgg tggcgaatcc gttctggcgg gtagcatcat cgttcgtcaa
    28261 cgtggtacca aattccacgc tggcgctaac gtaggttgcg gtcgtgacca cactctgttt
    28321 gctaaagcag acggtaaagt gaaattcgaa gttaaaggcc cgaaaaaccg taaatttatc
    28381 agcatcgaag ctgaataagt ttttcgcgtc ccggtaacgg atgaaagccc cgcaacacgt
    28441 tgcggggctt tttacattcg ataaccggta aaaatgcgca tggtgcgacg ttaatccgca
    28501 aatgcgcttt gcagggaata cggtatgaag cagcaggcag gcattggcat tcttttggcg
    28561 cttaccacag caatttgctg gggggcgttg ccaatcgcaa tgaagcaggt gctggaggtg
    28621 atggaacctc cgacaatcgt gttttaccgt ttcttgatgg cgagtattgg ccttggtgcc
    28681 attcttgcgg tgaagaagag gttgccgcca ttacgcgtgt ttcgtaagcc acgctggttg
    28741 attttgttgg cagtggcgac cgccgggctg tttgggaact tcatcctgtt cagctcatcc
    28801 ttgcaatacc tgagtccgac cgcttcgcag gtgattgggc aactctcgcc agttggcatg
    28861 atggttgcca gcgtatttat cctgaaagag aaaatgcgca gcactcaggt tgtaggggca
    28921 ttgatgctcc tgagcggtct ggtgatgttt tttaacacca gtctggtcga gatatttaca
    28981 aagctcaccg attacacctg gggagttatc tttggggtcg gtgcggcgac ggtttgggtg
    29041 agttatggcg tggcgcaaaa ggttttattg cgtcggctgg cctcaccgca gatcctgttt
    29101 ttactgtaca ctttatgtac aatcgcgctc ttccctctgg caaagcctgg agtgatagcg
    29161 cagcttagcc actggcagct cgcatgttta attttttgcg gactgaatac cttggtagga
    29221 tatggcgcct tggcggaagc gatggctcgc tggcaggcag cgcaggtgag cgcgatcatc
    29281 acgctcaccc cactgtttac gctgtttttt tcagatcttt tatcactggc ctggcccgat
    29341 ttcttcgcca gaccgatgtt aaacctttta ggttatctcg gtgcgtttgt cgtggttgcg
    29401 ggcgcgatgt attccgccat tggtcatcgt atttggggcg gattacgtaa gcatacaacg
    29461 gtggtatcgc aaccccgcgc aggcgaatga tttacggaga ataaaatgaa gtttgttgat
    29521 gaagcatcga ttctggtcgt tgcaggtgat ggcggtaatg gttgcgtgag cttccgccgc
    29581 gaaaagtata ttccgaaagg cggcccggat ggcggcgacg gcggtgatgg tggtgacgta
    29641 tggatggaag ccgacgagaa cctgaacacg cttatcgatt atcgttttga aaaatctttc
    29701 cgtgcagagc gcggtcagaa cggcgcaagc cgcgattgta ccggtaagcg cggtaaagac
    29761 gtgacgatta aagtgccggt aggtacgcgt gtaatcgacc agggtactgg tgaaaccatg
    29821 ggtgatatga ctaaacacgg tcagcgtctg ctggttgcta agggcggctg gcacggtctg
    29881 ggcaataccc gtttcaaatc gtcagttaac cgtacaccac ggcagaaaac taacggtacg
    29941 ccgggcgata agcgtgagct gctgctggag ctgatgctgc tggctgacgt cggtatgttg
    30001 gggatgccaa acgcgggtaa atcgaccttt attcgtgcgg tatcggcggc taaaccgaaa
    30061 gtggcggatt atccgtttac cactctggtg ccaagtctgg gtgtggtacg aatggacaac
    30121 gaaaagagct tcgttgttgc cgatattcca ggactgattg aaggcgctgc ggaaggcgca
    30181 ggtctgggca ttcgcttcct gaagcacctg gaacgttgcc gcgtcctgtt gcacctcatc
    30241 gatatcgatc cgattgacgg caccgatccg gttgaaaacg cgcgtattat tatcagcgag
    30301 ctggaaaaat acagccagga tctggcggcg aaaccgcgtt ggttagtctt caacaagatc
    30361 gatctgctgg ataaggcaga agccgaagag aaagcgaaag cgatcgctga agcgctgggc
    30421 tgggaagata aatattatct gatctctgcg gcgagcggac tgggcgtgaa agatctctgc
    30481 tgggatgtga tgacctttat cattgaaaac ccggtcgtgc aggctgaaga agcgaaacag
    30541 ccagagaaag tcgaattcat gtgggatgat tatcaccgtc agcagcttga agagattgct
    30601 gaagaggatg atgaagactg ggatgacgac tgggacgaag acgacgaaga aggcgttgag
    30661 ttcatttaca agcgttaatc actaatgacc aatgccaaat aagcctgatg cgctacactt
    30721 atcaggccta caagatgatt gcaatatatt gaatttgcac gattttgtag gccggataag
    30781 gcgttcacgc cgcatccggc ataaacaaag cgcactttgt caataatctg aaaccccggc
    30841 catgcgccgg ggtttctttt tgactaattg ttctgataaa tatctttgta caaacggctt
    30901 tcaaaacgca ctaacggaat acggcgatta cgctgatccg cgggttttac tgcatagcca
    30961 gaaagatatt gtacaaatgc cattcgttgc ccgctcgctg tggtaatgaa tcccgccagg
    31021 ttatataccc cctgcaacga accggttttc gctgagactt tgccatccac gcccgcctga
    31081 tgcagacctg cacggtactg caaagagccg tcatagcccg caagcggcag catggagata
    31141 aagttaagtt cattgtcgtg ttgggcaatg tattgcagca cctgcatcat ggtggcgggg
    31201 gcaatcaggt tatgccgcga aagccctgaa ccatcggcaa taatggtgtt accaatatcg
    31261 acaccggctt gctggcgcag gatctgacgc acggcgtccg acccagcccg ccatgttcca
    31321 ggcacattga agcgcgcatg gccgatcatg cggaaaaccg tatcggcgat catgttgtcc
    31381 gactttttca gcataatctt aagcagatcg tgcagcgggg ccgactgttt actggcaact
    31441 accgttccag gttcgttaac ctgagtctgg cgcagcagtg ttccgctcca ggtgataccc
    31501 gcctgtttta actcatcttt cagaattgca ccggcatagc tggctccatc ctgcacggca
    31561 aaagccaacg ggagcggctc agaacgttgt ggcaggcatc ccgtcagcgt aaagcggttc
    31621 aggtcgcttg gcaccacatc cagttcgcag tattgcgctt cggcagaacc acgggggagg
    31681 gtgcgtacct ggctgaacat cgtaacgggg taataagatg ccacgcgtat aaaagccata
    31741 tcaccaggct ttggggcact gtagagcgag acggagaaac agttgcggtc aactatggcg
    31801 gcagcaggcg gagcgctaaa gcattgtgtc atgtcattcc atggccagcc gggggcttta
    31861 tcgtggctgg cgaaaatgga ggtatctatc aacacattgc catcgatttg gttgacgcca
    31921 gattttttca aagtcgcgac catattgcga atatcctgac gttttaacgt cggatcggca
    31981 ccaaatcgcg ccactaagtc acccttaagt acgccgtttt ccacattgcc tttggtttca
    32041 agcgtcgtgg tgaaacgaaa atcggggccg agttgaatca acgccgccag cgcagtaatc
    32101 actttctggg tactggcggg cagcgccatc tgctgactgt ggtaatcaat agcgggggcc
    32161 gacgcaccga ctttttgcac catcagggca aggttggcac ctgcggggag ttgagtaacg
    32221 tactcatcaa cattcgcggc ctgaacactg aacgctatac agctggtcaa tccgatgata
    32281 aatctggaaa atcgcataat ctcgcgctaa caacctggaa tcgagccgtc atactacggc
    32341 gcaacgccct ataaagtaaa cgatgaccct tcgggaactt cagggtaaaa tgaccatcaa
    32401 aatgtgaatt gtagctgacc tgggacttgt acccgggtcg gtattttttt gcttctggtc
    32461 ccggtgagga gttatgccgg gcaggccgaa cagccggggt gggtgaagac ttgccccatc
    32521 aggaatattc aagaggtata acaaatgcaa gctattccga tgaccttacg cggcgctgaa
    32581 aaattacgcg aagagctgga ttttctgaaa tctgtgcgcc gtcctgaaat cattgctgct
    32641 atcgcggaag cgcgtgagca tggcgacctg aaagaaaacg ccgaatacca cgcagctcgt
    32701 gaacagcagg gcttctgcga aggccgtatc aaagacatcg aagccaagct gtcaaacgcg
    32761 caggtgattg atgtcaccaa aatgcccaac aatgggcgcg ttatttttgg tgctaccgta
    32821 acggtgctga atctggattc tgacgaagaa cagacttatc gcatcgttgg cgatgacgaa
    32881 gctgacttta aacaaaacct gatttctgta aactcgccta ttgctcgtgg cctgatcggc
    32941 aaagaagaag atgatgttgt ggtcatcaaa acgccgggcg gcgaagtaga atttgaagta
    33001 attaaggtgg aatacctgta agaattaccc aatactcaag atgttgatgt attgtaaaga
    33061 aaggaaaaag gccgctctgc ggccttttat caacgaacag agcgtggcat tttgctctcc
    33121 tgcctgcgga aaacccctcg ttttacacag caaatgtgtg taactttagg ataatcttag
    33181 cgtggcagcg agattttacg ttctttagtt gggcgataaa gcaccagcgt tttaccgatg
    33241 acctgtacat tacaggcgcc ggtttcgcgc acgatagctt ccacgatcaa ggtttttgtt
    33301 tcgcgatctt cggtggcgat tttcaccttg atgagttcat ggtgctctaa cgcttgttca
    33361 atctcggcca gcaccccttc ggtcaaacca ttactgccaa gcagaacaac tggcttgagc
    33421 ggatgtgcca gacctttcag gtgctgtttt tgtttagtac tcagattcat cgtatttttt
    33481 gcttacgttg ggattgaaaa cgggtcattc taccgccatc tcccatatat caccaaatag
    33541 gcgcgtaaaa atttacgcaa ttggttacga tgagttatcc ccatgggaaa gttaaatgac
    33601 aggtaagaag cgttctgcca gctccagccg ctggcttcag gaacacttta gcgataaata
    33661 tgttcaacag gcacagaaaa agggattacg ttcccgtgcc tggtttaaac ttgatgaaat
    33721 acagcaaagt gacaaactct ttaaaccggg aatgacggtt gtcgaccttg gtgctgctcc
    33781 gggtggttgg tcacaatatg tggtcaccca aattggcggc aaaggccgca tcatcgcttg
    33841 cgatctttta cctatggatc ctatcgttgg tgtggacttt cttcagggcg attttcgtga
    33901 tgaactggtg atgaaagcac tgctggagcg cgttggcgac agcaaagtcc aggttgtcat
    33961 gtccgatatg gcaccaaaca tgagcggaac accggcggtg gatatccccc gagccatgta
    34021 tctggtggaa ctggcgctag aaatgtgtcg tgatgtatta gcgccaggtg gcagttttgt
    34081 agtgaaggtg ttccagggcg aaggtttcga tgagtatcta agggaaattc gctccctgtt
    34141 tacgaaggtc aaagttcgta agccggactc ttctcgtgca cgttcgcgag aagtgtatat
    34201 tgtagcgacc gggcgtaaac cctaaccggg agatttcaga cgaaagtttg aaagatgctg
    34261 gatatagagt atcctgacgc tgtttttaac acagttgtaa taagaggtta atcccttgag
    34321 tgacatggcg aaaaacctaa tactctggct ggtcattgcc gttgtgctga tgtcagtatt
    34381 ccagagcttt gggcccagcg agtctaatgg ccgtaaggtg gattactcta ccttcctaca
    34441 agaggtcaat aacgaccagg ttcgtgaagc gcgtatcaac ggacgtgaaa tcaacgttac
    34501 caagaaagat agtaaccgtt ataccactta cattccggtt caggatccga aattactgga
    34561 taacctgttg accaagaacg tcaaggttgt cggtgaaccg cctgaagaac caagcctgct
    34621 ggcttctatc ttcatctcct ggttcccgat gctgttgctg attggtgtct ggatcttctt
    34681 catgcgtcaa atgcagggcg gcggtggcaa aggtgccatg tcgtttggta agagcaaagc
    34741 gcgcatgctg acggaagatc agatcaaaac gacctttgct gacgttgcgg gctgcgacga
    34801 agcaaaagaa gaagttgctg aactggtaga gtatctgcgc gagccgagcc gcttccagaa
    34861 actcggcggt aagatcccga aaggcgtcct gatggtcggt cctccgggta ccggtaaaac
    34921 gttgctggcg aaagcgattg caggcgaagc gaaagttccg ttctttacta tctccggttc
    34981 tgacttcgta gaaatgttcg tcggtgtggg tgcatcccgt gttcgtgaca tgttcgaaca
    35041 ggcgaagaaa gcggcaccgt gcatcatctt tatcgatgaa atcgacgccg taggccgcca
    35101 gcgtggcgct ggtctgggcg gtggtcacga tgaacgtgaa cagactctga accagatgct
    35161 ggttgagatg gatggcttcg aaggtaacga aggtatcatc gttatcgccg cgactaaccg
    35221 tccggacgtt ctcgacccgg ccctgctgcg tcctggccgt ttcgaccgcc aggttgtggt
    35281 cggcttgcca gatgttcgcg gtcgtgagca gatcctgaaa gttcacatgc gtcgcgtacc
    35341 attggcaccc gatatcgacg cggcaatcat tgctcgtggt actcctggtt tctccggtgc
    35401 tgacctggcg aacctggtga acgaagcggc actgttcgct gctcgtggca acaaacgcgt
    35461 tgtgtcgatg gttgagttcg agaaagcgaa agacaaaatc atgatgggtg cggaacgtcg
    35521 ctccatggtg atgacggaag cgcagaaaga atcaacggct taccacgaag cgggtcatgc
    35581 gattatcggt cgcctggtgc cggaacacga tccggtgcat aaagtgacga ttatcccacg
    35641 cggtcgtgcg ctgggtgtga ctttcttctt gcctgagggc gacgcaatca gcgccagccg
    35701 tcagaaactg gaaagccaga tttctacgct gtacggtggt cgtctggcag aagagatcat
    35761 ctacgggccg gaacatgttt ctaccggtgc gtccaacgat attaaagttg cgaccaacct
    35821 ggcacgtaac atggtgactc agtggggctt ctctgagaaa ttgggtccac tgctgtacgc
    35881 ggaagaagaa ggtgaagtat tcctcggccg tagcgtagcg aaagcgaaac atatgtccga
    35941 tgaaactgca cgtatcatcg accaggaagt gaaagcactg attgagcgta actataatcg
    36001 tgcgcgtcag cttctgaccg acaatatgga tattctgcat gcgatgaaag atgctctcat
    36061 gaaatatgag actatcgacg caccgcagat tgatgacctg atggcacgtc gcgatgtacg
    36121 tccgccagcg ggctgggaag aaccaggcgc ttctaacaat tctggcgaca atggtagtcc
    36181 aaaggctcct cgtccggttg atgaaccgcg tacgccgaac ccgggtaaca ccatgtcaga
    36241 gcagttaggc gacaagtaag ttcccgcatc agatgactgt atttgtaccg aaaaccccgg
    36301 ggcgtgctcc ggggtttttt cttatcaatt cataccaggg ataacataat gaaactcttt
    36361 gcccagggta cttcactgga ccttagccat cctcacgtaa tggggatcct caacgtcacg
    36421 cctgattcct tttcggatgg tggcacgcat aactcgctga tagatgcggt gaaacatgcg
    36481 aatctgatga tcaatgctgg cgcgacgatc attgacgttg gtggcgagtc cacgcgccca
    36541 ggggcggcgg aagttagcgt tgaagaagag ttgcaacgtg ttattcctgt ggttgaggca
    36601 attgctcaac gcttcgaagt ctggatctcg gtcgatacat ccaaaccaga agtcatccgt
    36661 gagtcagcga aagttggcgc tcacattatt aatgatatcc gctccctttc cgaacctggc
    36721 gctctggagg cggctgcaga aaccggttta ccggtttgtc tgatgcatat gcagggaaat
    36781 ccaaaaacca tgcaggaagc tccgaagtat gacgatgtct ttgcagaagt gaatcgctac
    36841 tttattgagc aaatagcacg ttgcgagcag gcgggtatcg caaaagagaa attgttgctc
    36901 gaccccggat tcggtttcgg taaaaatctc tcccataact attcattact ggcgcgcctg
    36961 gctgaatttc accatttcaa cctgccgctg ttggtgggta tgtcacgaaa atcgatgatt
    37021 gggcagctgc tgaacgtggg gccgtccgag cgcctgagcg gtagtctggc ctgtgcggtc
    37081 attgccgcaa tgcaaggcgc gcacatcatt cgtgttcatg acgtcaaaga aaccgtagaa
    37141 gcgatgcggg tggtggaagc cactctgtct gcaaaggaaa acaaacgcta tgagtaatcg
    37201 taaatatttc ggtaccgatg ggattcgtgg tcgtgtaggg gatgcgccga tcacacctga
    37261 ttttgtgctt aagctgggtt gggccgcggg taaagtgctg gcgcgccacg gctcccgtaa
    37321 gattattatt ggtaaagaca cgcgtatttc tggctatatg ctggagtcag cactggaagc
    37381 gggtctggcg gcagcgggcc tttccgcact cttcactggc ccgatgccaa caccggccgt
    37441 ggcttatctg acgcgtacct tccgcgcaga ggccggaatt gtgatatctg catcgcataa
    37501 cccgttctac gataatggga ttaaattctt ctctatcgac ggcactaaac tgccggatgc
    37561 ggtagaagag gccatcgaag cggaaatgga aaaggagatc agctgcgttg attcggctga
    37621 actgggtaaa gccagccgta tcgttgatgc cgcgggtcgc tatatcgagt tttgcaaagc
    37681 cacgttcccg aacgaactta gcctcagtga actgaagatt gtggtggatt gtgcaaacgg
    37741 tgcgacttat cacatcgcgc cgaacgtgct gcgcgaactg ggggcgaacg ttatcgctat
    37801 cggttgtgag ccaaacggtg taaacatcaa tgccgaagtg ggggctaccg acgttcgcgc
    37861 gctccaggct cgtgtgctgg ctgaaaaagc ggatctcggt attgccttcg acggcgacgg
    37921 cgatcgcgtg attatggttg accatgaagg caataaagtc gatggcgatc agatcatgta
    37981 tatcatcgcg cgtgaaggtc ttcgtcaggg ccagctgcgt ggtggtgctg tgggtacatt
    38041 gatgagcaac atggggcttg aactggcgct gaaacagtta ggaattccat ttgcgcgcgc
    38101 gaaagtgggt gaccgctacg tactggaaaa aatgcaggag aaaggctggc gtatcggtgc
    38161 agagaattcc ggtcatgtga tcctgctgga taaaaccact accggtgacg gcatcgttgc
    38221 tggcttgcag gtgctggcgg cgatggcacg taaccatatg agcctgcacg acctttgcag
    38281 cggcatgaaa atgttcccgc agattctggt taacgtacgt tacaccgcag gtagcggcga
    38341 tccgcttgag catgagtcag ttaaagccgt gaccgcagag gttgaagctg cgctgggcaa
    38401 ccgtggacgc gtgttgctgc gtaaatccgg caccgaaccg ttaattcgcg tgatggtgga
    38461 aggcgaagac gaagcgcagg tgactgaatt tgcacaccgc atcgccgatg cagtaaaagc
    38521 cgtttaaagc gttagataac tggctaaaaa ggcggcgatt gtcgcctttt ttctcggcga
    38581 acacatcggt tttgctgttt ttttccgcag ttgatacaat gcgataaaat tgcccttgcg
    38641 aaggtcattc gttttggtta gtattcacac ccgcttcagt gggaaagatt aaaaactccc
    38701 gctttattgg ttgaagcatt ggtacgcggc aactccgcaa ggaacaggtt gattatgtac
    38761 gaagctcttt tagtagtttt ccttattgtg gcaattggcc ttgttggtct gatcatgctg
    38821 cagcaaggta aaggcgctga tatgggagcc tccttcggag caggcgcttc cgctacgctg
    38881 tttggttcaa gtggttctgg taacttcatg acccgcatga cggcgctgct ggcaacgtta
    38941 ttcttcatca tcagtctggt gctgggtaac atcaatagca acaaaaccaa tagaggtagc
    39001 gaatgggaaa atctgagtgc accggcgaaa accgaacaaa ctcagccagc tgctccggct
    39061 aagccgacca gcgatatccc gaactaaaaa gtagtatccg tgccgaggtg gtggaattgg
    39121 tagacacgct accttgaggt ggtagtgccc aatagggctt acgggttcaa gtcccgtcct
    39181 cggtaccaaa ttccagaaaa gagacgctga aaagcgtctc ttttcgtttt ggtcctggtc
    39241 tgatgaatgg ttgtcaggta tctgtatctc gtccagttgc tttactcgta gctggtacct
    39301 tgaaacgatg gtgccggtac gccttagtta taaacttttc cctactgctg acataattgg
    39361 cactttattg tgtcaactcc agttccggta cgaatacgga tatgtttact tctcgataaa
    39421 ccatcctgct cctgtcagta taactactga tggctacgtt ctgcgtaatg tttagagacg
    39481 cattgataat gttatttaat tactgagaac gcaatggtcg catcatttaa aaagaaaaaa
    39541 ctaacagtgt ttcggtctga tatggatcag agttgaaaaa catgtctgat ataaaattga
    39601 acattgttga taattgttta tattggttgt gaacttggat ttctggatga cagtattcaa
    39661 caaatttgct agaagatttt aaatctcatt ggttgttgta tctttgtgtt attgtttttg
    39721 gtattacgaa cttagtcgcc tcttccggag cgcatatggt tcagcgattg ctgttcttcg
    39781 tcctgaccat cctggttgtt aaacgtatat catcccttcc gcttcgcctg cttgttgccg
    39841 caccatttgt gttactgact gcggcagaca tgagtattag cctctattca tggtgtacct
    39901 ttggtacaac tttcaatgat ggatttgcga ttagtgtgct gcagagtgat ccggatgaag
    39961 ttgtgaaaat gctggggatg tatatccctt atctatgtgc ttttgctttt ttatcccttc
    40021 tttttttggc agtaataata aaatatgatg tttccttgcc gacaaaaaaa gtgacaggaa
    40081 tattattgct gattgtcatt tcgggcagtt tattttccgc ttgtcaattt gcttataaag
    40141 atgcaaaaaa taaaaaagcg ttcagtccat atatactagc gtcgtgattt gctacctata
    40201 cgccgttttt caatctcaac tattttgctt tagcagcgaa agcgcatcaa agattactct
    40261 caattgcaaa cacggtgccg tattttcaat tatcagtcag ggatacaagt attgatacct
    40321 acgtgttgat tgtgggggag tctgtacgtg tcgacaatat gtctttgtat ggatatacac
    40381 gctctacgac accgcaagtt gaagcacaaa gaaaacagat caaactgttt aatcaagcaa
    40441 taagcggcgc accttacact gcgctgtcgg ttcccctttc tttaactgct gattctgttt
    40501 tgagtcatga cattcataat taccccgaca acattattaa tatggctaat caagcaggat
    40561 ttcagacttt ctggctaagc tcgcaatccg cttttcggca gaatggtaca gcagttacca
    40621 gtatcgccat gcgcgccatg gaaacagttt atgtcagagg atttgatgaa ttgttgttgc
    40681 cgcatttatc gcaagcatta cagcaaaata cgcagcaaaa gaaactgatt gttcttcatt
    40741 taaatggaag ccatgaaccg gcttgtagcg cctatccgca atccagcgcc gtgtttcaac
    40801 cgcaggacga tcaggatgcc tgctatgaca actccattca ttacacagat agtttgctag
    40861 gtcaggtttt tgaattatta aaagatcgcc gcgcctcggt catgtatttt gccgaccacg
    40921 gcctggaacg tgaccctacg aagaagaacg tctattttca tggaggcagg gaggctagcc
    40981 agcaggcata tcatgtcccg atgtttatct ggtatagccc cgttcttggg gatggcgtgg
    41041 atcgcacaac ggaaaacaat atcttttcga cagcttacaa taattacctt attaatgcgt
    41101 ggatgggggt aacaaagccg gaacagccgc aaacgcttga ggaagtgatt gcacactata
    41161 aaggagactc acgggttgtg gatgcaaacc atgatgtttt cgattatgtg atgctcagaa
    41221 aggagtttac agaggataag caaggtaacc ccacccctga agggcagggt tgatgtcgaa
    41281 ttactggcct ttgttttcca tattctccac ctgcggcagg cctgaagtgg cagaggagga
    41341 aagcaggcca gttttggcat aaccaaaaag tttctctcgg gtatcagtga tatccagatt
    41401 acgcatggtc aattgaccaa tacgatcatc tggcgagaac accgagtcgc ctttttccat
    41461 cgtcagacgc tctggcttgt aggtcaggtt ctctgagacg gtattcagga ttgaataatc
    41521 gttcccacgg cgcagctcca gggtaacttc accagtgatc tggctggcaa cccagcgttg
    41581 cagagagtca cgcagcatca gcgcctggga gtcaaaccaa cgcccctggt acagcagacg
    41641 gcccaactga cgaccatgcg cgtgatactg ctcaatggtg tcttcgttgt gaataccggt
    41701 caacagacgt tcatacgcaa tgtgcagcag tgccatcccc ggagcttcgt aaataccacg
    41761 gcttttcgct tcgatgatac ggttctcaat ctggtcgctc atgcccaggc cgtgacgacc
    41821 gccgatgcgg ttagcttcca gcatcatttc tacgtcgtcg ctaaaggttt taccgttcag
    41881 cgccaccgga tgaccttgct caaagcgtac tgtgacttct tctgccggga tcttcacgct
    41941 ctcatcccag aatttcacgc ccataatcgg gttgacgatt ttgacgctgg agttgaggta
    42001 ttccagatcc ttcgcttcat gcgttgcacc aagcatgttg gagtctgtgg agtaggcttt
    42061 ttcgacagac attttgtagt cgaaaccgca ggcaatcata aattcagaca tctcatgacg
    42121 gccgcccagt tcatcaataa agtcagtatc aagccacggt ttgtaaatct gcagttcagc
    42181 attggtcagc agaccataac gatagaaacg ttcgatatcg tttcctttat aggtgctacc
    42241 gtcaccccag atattcacgc catcttcttt catcgcagca accagcatgg taccagtcac
    42301 ggcgcggccc agcggcgtcg tgttgaaata ggtcaggccg ccggtggtgt tatgaaatgc
    42361 gccacactga atagcggcaa taccttcggc caccagttgt ttgcggcagt cgatcagacg
    42421 tgcgttctcc gcgccgtatt ccatggcacg acgagggatc gcatcataat cctcttcgtc
    42481 tggctggccc aggtttgcag tatatgcata aggaaccgct cccttttgtc gcatccacag
    42541 cagtgcggca ctggtgtcca gaccgccgga aaaagcgata ccaatacgtt gacctaccgg
    42601 gagatgcttg agaatcgtcg tcataaaata acaccctgct taattaactg atgatgagcc
    42661 tggatttccg ctctcactga atttttatgc aaaataaatg agttttcatt taatcatctt
    42721 ttatcggaga caggaagagt ttagtgtgtt ttttgtaaaa taatgcgctt aagggagagc
    42781 aggggaaggc aaaagtattc aacaaatgaa agtgaactgg atattcattc acgtgattag
    42841 caataaacgt tgacaaaatg tggcatggat cactataatg cctgcagatt ttacgtcccg
    42901 tctcggtaca ccaaatccca gcagtatttg cattttttac ccaaaacgag tagaatttgc
    42961 cacgtttcag
//
LOCUS       DAJTSI010000018        42599 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000018 DAJTSI010000000
VERSION     DAJTSI010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.018"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..579)
                     /gene="tolA"
                     /locus_tag="OO327_000871"
     CDS             complement(<1..579)
                     /gene="tolA"
                     /locus_tag="OO327_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR6017509.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAE"
     gene            complement(644..1072)
                     /gene="tolR"
                     /locus_tag="OO327_000872"
     CDS             complement(644..1072)
                     /gene="tolR"
                     /locus_tag="OO327_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR6017510.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(1076..1768)
                     /gene="tolQ"
                     /locus_tag="OO327_000873"
     CDS             complement(1076..1768)
                     /gene="tolQ"
                     /locus_tag="OO327_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR6017511.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(1765..2169)
                     /gene="ybgC"
                     /locus_tag="OO327_000874"
     CDS             complement(1765..2169)
                     /gene="ybgC"
                     /locus_tag="OO327_000874"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6017512.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(2319..2612)
                     /gene="ybgE"
                     /locus_tag="OO327_000875"
     CDS             complement(2319..2612)
                     /gene="ybgE"
                     /locus_tag="OO327_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR6017513.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(2612..2725)
                     /gene="cydX"
                     /locus_tag="OO327_000876"
     CDS             complement(2612..2725)
                     /gene="cydX"
                     /locus_tag="OO327_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR6017514.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(2740..3879)
                     /gene="cydB"
                     /locus_tag="OO327_000877"
     CDS             complement(2740..3879)
                     /gene="cydB"
                     /locus_tag="OO327_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6017515.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(3895..5463)
                     /gene="cydA"
                     /locus_tag="OO327_000878"
     CDS             complement(3895..5463)
                     /gene="cydA"
                     /locus_tag="OO327_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR6017516.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQTVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(6312..8945)
                     /gene="mngB"
                     /locus_tag="OO327_000879"
     CDS             complement(6312..8945)
                     /gene="mngB"
                     /locus_tag="OO327_000879"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCR6017517.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRLKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLARTLGFGYHHGLLEKMWKEILK
                     NHAHDSIGCCCRDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVDVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQESWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(8963..10879)
                     /gene="mngA"
                     /locus_tag="OO327_000880"
     CDS             complement(8963..10879)
                     /gene="mngA"
                     /locus_tag="OO327_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR6017518.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            11049..11771
                     /gene="mngR"
                     /locus_tag="OO327_000881"
     CDS             11049..11771
                     /gene="mngR"
                     /locus_tag="OO327_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR6017519.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(11874..12743)
                     /gene="sucD"
                     /locus_tag="OO327_000882"
     CDS             complement(11874..12743)
                     /gene="sucD"
                     /locus_tag="OO327_000882"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR6017520.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(12743..13909)
                     /gene="sucC"
                     /locus_tag="OO327_000883"
     CDS             complement(12743..13909)
                     /gene="sucC"
                     /locus_tag="OO327_000883"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR6017521.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(14003..15220)
                     /gene="odhB"
                     /locus_tag="OO327_000884"
     CDS             complement(14003..15220)
                     /gene="odhB"
                     /locus_tag="OO327_000884"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR6017522.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(15235..18036)
                     /gene="sucA"
                     /locus_tag="OO327_000885"
     CDS             complement(15235..18036)
                     /gene="sucA"
                     /locus_tag="OO327_000885"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR6017523.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(18337..19053)
                     /gene="sdhB"
                     /locus_tag="OO327_000886"
     CDS             complement(18337..19053)
                     /gene="sdhB"
                     /locus_tag="OO327_000886"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR6017524.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(19069..20835)
                     /gene="sdhA"
                     /locus_tag="OO327_000887"
     CDS             complement(19069..20835)
                     /gene="sdhA"
                     /locus_tag="OO327_000887"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR6017525.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(20835..21182)
                     /gene="sdhD"
                     /locus_tag="OO327_000888"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(20835..21182)
                     /gene="sdhD"
                     /locus_tag="OO327_000888"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR6017526.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(21176..21580)
                     /gene="sdhC"
                     /locus_tag="OO327_000889"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(21176..21580)
                     /gene="sdhC"
                     /locus_tag="OO327_000889"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6017527.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            21961..22068
                     /locus_tag="OO327_000890"
     CDS             21961..22068
                     /locus_tag="OO327_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6017528.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            22274..23557
                     /gene="gltA"
                     /locus_tag="OO327_000891"
     CDS             22274..23557
                     /gene="gltA"
                     /locus_tag="OO327_000891"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR6017529.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            23948..24514
                     /locus_tag="OO327_000892"
     CDS             23948..24514
                     /locus_tag="OO327_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6017530.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            24565..27024
                     /locus_tag="OO327_000893"
     CDS             24565..27024
                     /locus_tag="OO327_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6017531.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            27039..27770
                     /locus_tag="OO327_000894"
     CDS             27039..27770
                     /locus_tag="OO327_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6017532.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            27767..28843
                     /locus_tag="OO327_000895"
                     /pseudo
     CDS             27767..28843
                     /locus_tag="OO327_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            28995..30041
                     /locus_tag="OO327_000896"
     CDS             28995..30041
                     /locus_tag="OO327_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR6017533.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(30038..30829)
                     /gene="nei"
                     /locus_tag="OO327_000897"
     CDS             complement(30038..30829)
                     /gene="nei"
                     /locus_tag="OO327_000897"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR6017534.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(30865..31599)
                     /gene="pxpA"
                     /locus_tag="OO327_000898"
     CDS             complement(30865..31599)
                     /gene="pxpA"
                     /locus_tag="OO327_000898"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR6017535.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(31589..32521)
                     /gene="pxpC"
                     /locus_tag="OO327_000899"
                     /pseudo
     CDS             complement(31589..32521)
                     /gene="pxpC"
                     /locus_tag="OO327_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(32515..33171)
                     /gene="pxpB"
                     /locus_tag="OO327_000900"
     CDS             complement(32515..33171)
                     /gene="pxpB"
                     /locus_tag="OO327_000900"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR6017536.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(33194..33937)
                     /gene="ybgI"
                     /locus_tag="OO327_000901"
     CDS             complement(33194..33937)
                     /gene="ybgI"
                     /locus_tag="OO327_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR6017537.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            34208..35689
                     /gene="dtpD"
                     /locus_tag="OO327_000902"
     CDS             34208..35689
                     /gene="dtpD"
                     /locus_tag="OO327_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR6017538.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(35839..37257)
                     /gene="phrB"
                     /locus_tag="OO327_000903"
     CDS             complement(35839..37257)
                     /gene="phrB"
                     /locus_tag="OO327_000903"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR6017539.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRKWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(37254..37763)
                     /locus_tag="OO327_000904"
     CDS             complement(37254..37763)
                     /locus_tag="OO327_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR6017540.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            37892..38179
                     /locus_tag="OO327_000905"
     CDS             37892..38179
                     /locus_tag="OO327_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HCR6017541.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(38234..38614)
                     /locus_tag="OO327_000906"
     CDS             complement(38234..38614)
                     /locus_tag="OO327_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6017542.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(38617..42003)
                     /locus_tag="OO327_000907"
     CDS             complement(38617..42003)
                     /locus_tag="OO327_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6017543.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDDPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEF
                     GRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNR
                     TKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGA
                     PEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYD
                     PDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVT
                     VRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYLTENKTKAGSYHWDDIKDPSV
                     TKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPWET"
     gene            complement(42022..42225)
                     /locus_tag="OO327_000908"
                     /pseudo
     CDS             complement(42022..42225)
                     /locus_tag="OO327_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT        10309 a        11606 c        10289 g        10395 t
ORIGIN      
        1 ttctgccgct tccgctttct tcttcagtgc cgcagcggct acctcggctt ttttctgcgc
       61 ttcggctgcg gctttggcgg cttctgcttc tgcttttttc ttcgcgtctg cagccgcttt
      121 cttcgctgct tcttccgcag ctttagcatc tgccgccgct ttcgctgccg ccacttcagc
      181 ttgcttctgc tttaactcgg cctgttttgc ggcttcttca gcctgctttt tctgctcctg
      241 agccgctaac cgctctttct caagttgctt caggcgttcc tgttcagccg cttgtttctc
      301 acgcagttct tcagcagcct gctgttcctt catcttgcgc tgttcatcag aacgcttcgc
      361 gcttgattcc tggctttgca tgcgtttgta ctgctcaact accgcacctg aatcaaccat
      421 gacagcgtcg atggacgaac caccgccgcc tccggctgaa gcttctatat tctcatcgaa
      481 cgaactccag atcagcgccg caaataagat gacatgcagc actgctgaaa taattatcgc
      541 ccgcttgagc ttgtcgtttt gttcggttgc ctttgacact ctcggtttcc aaaaactgtt
      601 cgcctgttac ccgctctctt tcaagcaagg gaaacgcaga tgtttagata ggctgcgtca
      661 ttaaaccaac cgatttcaca cccgcactat gtaacaagtt cagtgcttta attatttcat
      721 cgtaaggcac atcttttgcg ccaccgatca gaaagaccgt tttcgggttg gccttgaaac
      781 ggctggacac ttccgccacc acctgctctg gtggtaaacg ctccaggcga tctttctcaa
      841 ccaccacggt gtactgacca ataccagaca cttcaacaat cactggcgga ttatcgttac
      901 tgctcaccgc ctgtgattca gtagcgtctg gcagatcgac ctccacgctc tgggtgatga
      961 tgggcgctgt cgccataaag atcagcaaca gcaccagcag tacgtccagc aacggaacaa
     1021 tgttgatttc ggacttgaga tcgcgacgac ctcgtccacg cgctctggcc atggcttacc
     1081 ccttgttgct ctcgctaacg gtaaacgcct ggcggtgcag aatcgcggta aactcttcca
     1141 taaagttgtc gtaattcagt tccagtttgt ttacgcgctg gttgagacgg ttataagcca
     1201 ttaccgccgg aattgcggcg aacagaccaa ttgccgtcgc aatcaacgct tctgcgatac
     1261 cgggcgcaac catttgcagt gttgcttgtt ttaccgcccc gagggcgata aaggcgtgca
     1321 tgatccccca gaccgtacca aacagaccaa tatacgggct gatggagcca accgtaccga
     1381 ggaacggaat gtgcgtttcc agattttcaa gttcacggtt catggagata cgcatagcac
     1441 gcgacgcccc ttccactacg gcttccggcg catggctatt ggcacgatgc aggcgcacaa
     1501 actctttgaa cccgctgtaa aagatttgtt ccgaaccagt cagattatcc cgtttcccct
     1561 gactctcttg atagaggcga gagagttcga ttccagacca gaatttatct tcaaacgctt
     1621 cggcttcacg cgccgctgcg ttaagaatac gggttcgctg gataataatg gcccaggatg
     1681 cgattgaaaa accaatcaaa atcaacatga taagtttaac cagaaggcta gccttcagga
     1741 acaaatcaag gatattcatg tcagtcactg cttaaactcc gcgacaatag acttgggaag
     1801 cgcacgaggc ttcattttga gtgggtcaac gcaaacaacc agaacctctg cttcattcag
     1861 caaagtattc tcggcgttga caatacgttg cgtgaaaacc aaagaggtgc cacgcattga
     1921 tgttatttca gtctgtattt cgagcatatc gtcgagccgc gcaggtgcgt aatattccac
     1981 cgtcatttta cgtaccacaa aggcaacgcg ttcagccatc agcgcctgct gactgaagtg
     2041 atgatgacgc agcatctctg tgcgtgctct ttcataaaaa gcgacgtaac tggcgtggta
     2101 caccacacca ccggcatcgg tatcttcata gtagacgcga accggccatc gaaacagcgt
     2161 tgtattcact ttacatcccg gtaatgcaac aaaagttaga gcttttaaac gctgctacta
     2221 tacgcgcgcg attgaggttt gggaatgagg gcaagttaag ggcgcgtaaa aatttatggg
     2281 cctagagcgg cccataattt tttaaagaga attctgattt aaaagaagaa gaaaatcagc
     2341 ccgacaatga gaacaatatc ggcaagcaat gggcaaaaaa tcccttgcca aagaaccttc
     2401 tgcggacgaa agcccacgcc gtgaatcaca ccagcacaga cggcccacat cagcaataaa
     2461 ccatgccaga tttccagttc actggtcttc gcggcaaaac gagatgggtc ccaaaacata
     2521 catcctgcta acagaagcgc catcacgaag gaaagcgccc gcagggggcg cttgtccatt
     2581 accgcataca aggtcgcgat aatcttactc atcagatgtc ttcttgaccg gctttgcctg
     2641 attcgacgtg ttcaagcgcc agcgcggtga ttaccccaaa cgaacaggca agaagcgttc
     2701 ccagaatcca tgcgaaatac cacattttta gctccttact tagtacagag agtgggtgtt
     2761 acgttcaata tcttctttgg tgatacgacc gaacattttc cagtaacacc aggcggtgta
     2821 gagcagaatg atcggtacca gaaccaccgc aacccaggtc atgacgttaa gcgtcagctg
     2881 gctggaagtt gcatcccaca ttgtcagact tgcgttcatc atggtgctgg acggcatcac
     2941 aaacgggaac attgcgatac cggctgtcag gatgatgcag gccagcgtca gggaggagaa
     3001 cacaaacgcc cacgcggctt tatccatacg tgcagtcagg atggtcagca gcagcagaac
     3061 cacacccagt gccggaatag cccacagaat tggcgtgttg ttgaagttaa ccagccatgc
     3121 gccagcttca cgaaccactt ctttattcag tgggttagag gctgcgtaat ggtccattgt
     3181 cgatttcacg acataaccat cgataccgta catcacccat acgccagcca gtgcgaaaca
     3241 gaccagtgtc accagcgcag ccacctgagc cgttgcacgg gtacgcaggt gcagttcgcc
     3301 cacggtacgc atttgcagat aggttgcgcc ctgagtaatg atcatcccta cgctcaccac
     3361 gcctgccagc aggccgaacg ggttaagcaa ctggaagaag ttaccggtgt agtacagacg
     3421 cagatattca tcaacgttga acggtacgcc ctgcaacagg ttaccgaacg ctacaccaat
     3481 taccagcggc ggaacgaagc taccaatgaa gatgccccag tcccacatgt tacgccagcg
     3541 ggtttcttca atcttggagc ggtagtcaaa accgaccgga cggaagaaca aagacgccag
     3601 cacgaggatc atcgccacat agaagccgga gaacgcagcg gcatagacca tcggccaggc
     3661 agcaaagagt gcgccgcccg cggtgatcag ccaaacctgg ttaccgtccc agtgtggtgc
     3721 aatggagtta atcataattc gacgctcggt gtcgttacga ccgaggaaac gggtgagcat
     3781 gcccaccccc atgtcgaaac cgtcagtgac tgcaaaacca atcagcagaa cgccaaccag
     3841 cagccaccag ataaaacgca atacttcata atcgatcatt tgacgactcc tgtcttagcg
     3901 tgccggctga gtagtcgtgg aagactgctc aaagtgatag cgaccggttt tcaggctgct
     3961 tgggccgagg cgtgcaaact tgaacattaa gaacaattct gccaccagga acagggtata
     4021 caggccgcaa atcagcacca ttgagaagat gagatcgcct gcggtcagtg acgaattcgc
     4081 cacagctgtc ggcagcactt caccgatagc ccacggttgg cggccatatt cagccacgaa
     4141 ccagcccgct tctacagcaa tccacggcag cggaataccg tacagcgcgg cgcgcagaag
     4201 ccattttttc tcgccaatgc ggttgcggat gacactccag aaagagagcg cgatgattgc
     4261 cagcagcagg aagccacacg ccaccatgat acggaacgca aagtacagcg gcgctacacg
     4321 cgggatggag tctttggttg cctgttgaat ctgcgcttca gtcgcatcag ccacgtttgg
     4381 cgtatagcgt ttcagcagca gaccgtaacc gaggtctttc ttcatgctat tgaactggtc
     4441 acgaaccgtc tggtcggtag aaccagaacg cagttgttcg agcagagagt acgccttcat
     4501 cccgttacga atgcgttctt catgctgcac catcagctct ttcaggccga taaccggggt
     4561 atccacggaa cgcgttgcaa tgatgcccag tgcgtaaggg atctgaatcg caaatttgtt
     4621 cgtctcctct tcctgatcag gaatgccgaa caaagtaaag gcagcaggcg caggttgcgt
     4681 ttcccactcg gcttcaatag cagccagttt ggttttctgc acgtcgccca tttcgtagcc
     4741 ggattcatca cccagaacaa taacagacag aacagcagcc ataccgaagc tggcagcgat
     4801 agcaaaggag cgtttagcga aggcgaagtc acgacctttc agcatatacc atgcgctgat
     4861 accgaggatg aacatcgcgc cagtcacata accagacgct acagtgtgaa cgaatttcac
     4921 ctgagcaacc gggttaagca ccagctcgga gaagctcacc atctccatac gcatagtttc
     4981 aaagttgaaa tcggacgcga ttgggttttg catccagccg ttcgcaacca gaatccacag
     5041 tgcggacagg tttgaaccga gcgccaccag ccaggtgaca cacatatgct gaactttacc
     5101 cagacgatcc caaccgaaga agaacagacc tacaaaggtg gattcgagga agaaggccat
     5161 cagaccttcg attgccagcg gcgcaccgaa gatatcccct acatagtggg aatagtaaga
     5221 ccagttagtc ccgaactgga actccatggt cagaccggta gccacaccca gagcgaagtt
     5281 gataccaaac aacttgcccc agaacttggt catatcttta taaatctgtt tgccggagag
     5341 gacgtagacc gtttccataa tggccaggag gaacgccata ccgagcgtca gtggcacaaa
     5401 aaggaagtgg tacatcgcgg tcaaggcaaa ctgtaagcgc gacagttcga ctatatctaa
     5461 catcatgact ccttgctcat cgcatgaaga ctccgagagt gaaccccgtt agaaagggtc
     5521 acacgcatgc cccaatagaa atttgtttga acactttcat cataccatgt gaatgcacac
     5581 aaaaacggcg taaagaaagg ttgcaaacat gttaataaaa actcaaattg atcccacgta
     5641 tatattacgc cgcaaaatcc ttacaataaa caggtttttg ttgaagcaaa tttgcatttt
     5701 tcgacggtga tcaatttata gctaaattgc cgcctttcag ccaatttgat cgagaacaat
     5761 ttatctcttt ttgatgccca tttccaagac ttatacattg ataaatatca atttctacaa
     5821 attatgttaa tcatgtttca acaaggtaac tttagtgata tttatgttta aaatgtgtga
     5881 attataagac tgaaattagt ttcggttggc aattaaagat aaaaagagac gaagcaaaaa
     5941 cagcacggtt gaccttaatt ctttttttgg tcactggcta atacgacgca gagtttcagg
     6001 attattaata aaggcataaa tctccctccc gccgatatta acatttaatt aacttataat
     6061 tatattataa ttaactcacc cgtggttatt attttgttaa aaataaagcc acatatacgt
     6121 agcttgagag tgaagataaa cacaaaatta cctgatgcgc tacgcttgcc agtgtctaca
     6181 tcatctctgc aatatattga atttgcctgc ttttgtagac cgaataaagc gttcatgccg
     6241 tatccggcat gaacaaagcg ccctttgtca acaatctggc cgcgcatatg tggcttcggt
     6301 ttgattgcta ttcaggcaag ccggtaactg aacgtccgtg actggccggg taaaaaaggc
     6361 cccgatagat ttgaaccttg attttcctcg gtggtaatgt gttcatccat catcgtttct
     6421 gagcaagaaa tcacctcgcg actgaaagca acagtcgcat cacaggttgc tgattcagcc
     6481 ggattaaaca gccgtaaaat cacttcttgt cggtcttcag ctttcttaag tgcgcttatc
     6541 aggcatccca ctgggggcat tttcaacaaa ctataacttt ccggcacgtt gaatccggct
     6601 ttgttgagct tcatcgcatc ccatgggatt ttgttgtaac actgtactgg agtcagccat
     6661 gctcgcgcct gctgagctac accagcggcg gttggcgtac cggtataact caataaactt
     6721 aggcgacaag aaagcagacc acgtagttgt gagtccggga ctggcatttt aattcccgaa
     6781 ggccgcctag gccttaaaag cagatcttct ttccccagta agcccacgcc gcgcagcaac
     6841 gtaatggcaa aggttttctt ctcttcaccg atgacttcaa attcacgtag cccttcgcta
     6901 aagacagcca tgccgttacg cccttcctgt aaggctgcat agttgagcat attccatacc
     6961 ggaaccggcg cttctttcca gctttcttgc tgccagttgt tcattgcact gtcgttcacg
     7021 gggcgcgtta gcgaaccaaa ctgggtatct gccagaacac tatcggtgtt aaaagatgta
     7081 gggatcagga cgcgaaggcg atgatcgtca gcctggttat caaggttgat atccacatca
     7141 atgcgcctgt tgttatgact aagagtgaca accaactcga catctactct gccagtggat
     7201 tgtcttgcgc tgcgttgttg cagattacgc ggcaccgcca tttcatagcg gataacagcc
     7261 ctgctctgcc aggcctcatg aataatctcg cattgcggtt tcgcattcgc cgaagtaatt
     7321 acccactctt cttttgcggg tgaatagtca tattcatcac catcatcaga gctttcttca
     7381 atttgcaaca cccggtcata gcgcacaccg ctatctttat ctaccagttg tagcgttcca
     7441 tcctcattga gcgcaatttg ccagaaagca ttttccagta tcccttcagc gtcacttttt
     7501 gccgcaatca cgttgccagg ctgattcgct tcgatataaa gcgtgcgata gcccatagaa
     7561 gggacaatct ggttgatctg tatatcaaac tccataaagg gatcgtaatt accgtaatga
     7621 actatttgcc gatcgattag gcctggatcg atctcacggg catggcgaat aaaatacggt
     7681 acaggctgac cgcgatcgtc tcgtagatta aactggctgg cgcgcagccg cacagtggtg
     7741 ttgatcactt cttcccgcgg ccagggcatc aggttaaaca ggacgagttt gtcggcgtcg
     7801 ctctgcggca tgttgtcggc gattttacgc atatagaaac gcagcagatt atccgccatg
     7861 tcttcagcca gttcgaagcg ggcgacgatt tcgcgatgaa ctttgtcacg acagcagcag
     7921 ccgatgctgt cgtgggcatg attttttaag atctctttcc acattttctc cagcaagccg
     7981 tggtggtatc caaaacccaa cgtcctggcc agcgttgcca gcggttccag cagattaaca
     8041 atcttatttt caatacgtgc gtgggcaact ttgatatcca tgcgcgtgga accgatggtg
     8101 cgatgcacgc gcatatattt gccatcaata aattcccctt tcagggttgc cagattatct
     8161 cgctgcgctt cgatcttctc aaatacctct tcaaagcggc tcatcacaaa ctttcgttga
     8221 ggataaattt cgcgcagttt ctccatcact tcgaagatat tttgctgcaa tggcatctga
     8281 tcatgcccat tcggcagcaa aatctctttg gttacagacg ctttttccag cacgtcaaaa
     8341 taactgtcga ggcgtttgcg taatccgttt tcgtccgcag gtaagtactt accgatggca
     8401 taacccaacg gcatcacctg cgccgtcact tcgctaccgt cactgctttg ccacaaaaac
     8461 tcggttttat ccgtaccgtg gcgctcagaa catccacgcc agaacatggt gcaggtaatg
     8521 ccaaatccat tgtagatatg cggcagttgc ccggacatgc caaaggaatc gggtaaataa
     8581 cctattttca tcggctcgcc aaacgcgagg cagtcacgca ttccgtacat cagattacgg
     8641 acgatggatt ccgcagaaac aatcgtggta tcggtctggg tataccaggg gccgataatc
     8701 aacttgccgg cttctaccag tttcttcaag cggtctttgt tttccggttt caccgcgaaa
     8761 taatcttcga ggatcgccgt ttgcccgtcg agtacgtaat atttgtattc gttgtcctgt
     8821 tccagtcggc acaggatctc ttccatatta ttgaccagca gaatgcgtga ctcttcggtg
     8881 gtgaaatacc actcccgatc ccagtgcata tgcggggtga tgtgaacgcg agataccgct
     8941 ttcatcgtcg tttccttttt gtttatggca ttacgccatc agtcagatag ttgccatgct
     9001 taaccgcgtg acgccgccag atcaggagaa ttgcagtcga gattgcagcc cccaccaatg
     9061 ccgcaccaaa ccagccaatt gccgccataa caccgcccgc gccattatca tgaagtaaaa
     9121 agagcgagaa aatgccagca ccgggtgtcg aaaggccgat attcatcgca ccgacaatag
     9181 cgcccgttac catagagccc agcacaaacg aaccaataac ccgcagcgga tcttcaatcg
     9241 ccatcgggat cgccccttcg gtaatacctg ccagccctaa cagccaggtg gatttcccgg
     9301 tctcaatttc aaactcttta aacaggcgcg gtgcgagcat cgtggaagcg gttacggtaa
     9361 atgccgaaac cattttgacg gaggcgaaaa tggcatacgg gccgtaaacg ccgttcgcca
     9421 ttgcgcccag gcagaatgca taagcggctt tattcactgg ccctccaagg tcaaaggaac
     9481 acataaaacc gagaatggca cccagcaaca gcgcgttact tcctgacaga ccgttcagcc
     9541 aggcggtaag cgagttattg atccaggcga caggttcccc caccacaaac agcatcagac
     9601 tgcccgctcc caatgtaccg agcaccgggt agagataaaa agtcaggaag ccattgaatt
     9661 tactgctaag acgcaagtga tttttcatcc agcgtatcaa gtaaccggct atcaatccgc
     9721 caacgaccgc gccgagaaac ccggagccga tcatgttggc ggcaagtcca gccgcaaagc
     9781 ctggcgctaa cgccggttta tctgccagcg aataggcggt ataggctgcc agcaccggta
     9841 ccatcaaaat tccgagcagc ccgccgccca gtttgcggta catccacagc caggagtttt
     9901 cttcattaaa cagatcttgt agcccgaaga tttgcgacag taataccgcg accgccagca
     9961 ccgtcccccc cgcgacaatc aacggtacgg caaaagagat cccgctcaac agtgcctgtt
    10021 tcagctccgt tttgacgctt ttcaccggtt gcgtatcttg ctgtaaggta cgcgtctcat
    10081 cgccacgctc gagggtaagc gattgttgga tcaacgcttc tgcatggcga atcggctcgg
    10141 caacaggcac agaaagcgca ggaatgccgt taaaacgctc actctccttg atggcgactt
    10201 cagccgcaaa aatacaggca gttgcactat tgagttgatc cgccgttaaa cgcccttcaa
    10261 taccgttagc accttgcttt tcaacgtaga cattcacgcc gagtttgcgt ccggcttttt
    10321 ccagatactc cgcagccatg taggtgtgag caatacccgc cgggcaggcc gttacgcaaa
    10381 cgatagttgg cgcgttggaa aaagaggcag aaggttgcgt gcctcccttg tcatccagcg
    10441 ccgagagcaa ttcatcaggc gtcgtcgccg actgtatacg cgcccgaatc tcatcatccg
    10501 ccaggcgcgt ggtcagcgct gtcagcagtt gcatgtgcgt agtacccgct tcattgaggg
    10561 gaatcgccag caacaccact aaatcaaccg cttccgggcc atccacgcct tcccactgaa
    10621 gcggctcgct gagtgtggcg accgcaaacg ccgcttcttt taccgcagca gttttgccat
    10681 gcggcacagc caacccttca cttaaggcgg tcgggccaag gctttcacgg cgatacactt
    10741 ctttcagaaa ttgctcagta ctggaaattt tccccagagc agcaagacgt tgagtcaacg
    10801 cgtgaatggc ctcttcacgg ctggtaaagc gcgcattcag acacaacgca tcgcggtggg
    10861 tcagagtcgt caggttcatc atgatcctca cttgatcgtt tttatagtcg cgccagtgtg
    10921 cccgacaaaa caataccatt gcgtaatcaa tttcacttga ttgaaattat ttgcatttta
    10981 tttgtattgt ttacaggatg agtttatcgg gcataatggc taaattcgta ttaatgagat
    11041 gctaaataat gggacacaaa cccttatacc gacagattgc tgatcgcatt cgtgagcaaa
    11101 ttgcgcgtgg cgagcttaaa cctggcgatg cgttacctac cgaatcggca ttacaaacag
    11161 agtttggcgt cagccgggtt acggtgcgtc aggctttgcg ccagttagtc gagcaacaga
    11221 tcctcgaaag cattcagggc agcgggactt acgtcaaaga agagcgggtc aattacgata
    11281 tttttcagtt aaccagtttt gatgaaaaac tgtcggaccg tcacgtcgat acacacagtg
    11341 aagttctgat atttgaagtg attccggctg acgattttct tcagcaacag ctacaaatca
    11401 ccgtgcagga tcgtgtttgg catgtgaagc gcgtgcgcta tcgcaagcaa aagccaatgg
    11461 cgctggaaga aacctggatg ccgcttgcgt tgttcccgga tctcacctgg caggtcatgg
    11521 aaaattcgaa atatcacttt attgaagaag tgaagaagat ggttatcgat cgtagcgaac
    11581 aggaaatcat tcctctgatg ccaaccgaag agatgagccg tctgcttaat atcagccaga
    11641 caaagccgat tcttgaaaaa gtatcgcgtg gatatttagt tgatggtcgg gtgtttgaat
    11701 atagccgcaa cgcttttaat accgatgact ataaatttac cctgatagcg cagcgaaaat
    11761 catcgcgata agcacaaaaa ggccatcata cgatggccaa ccatgtcgaa accgacagcc
    11821 cttcgccatt cctgtagagg gaagcggcga gggctatttc ttattacaga tatttatttc
    11881 agaacagttt tcagtgcttc accgatatcc gccaggctgc gaacggtttt cacgcctgcg
    11941 gcttccagag cagcgaattt ctcatccgca gtccctttcc caccggcaat gatcgcaccc
    12001 gcgtggccca tacgtttgcc tttcggcgca gtcacaccag cgatgtaacc cacaactggc
    12061 ttggtaacat gctctttgat gtacgctgcc gcttcttctt cagcgctacc gccgatctca
    12121 ccgatcatca cgatcgcttc ggtctgcgga tctttttcga acatttcgag aatgtcgata
    12181 aagttagagc cagggatcgg gtcaccgccg ataccgacac aggtcgactg accgaaaccg
    12241 taatccgtgg tctgtttaac cgcttcatag gtcagtgtac cggaacggga aacgataccc
    12301 actttacccg gtttgtgaat gtgacccggc tggataccaa ttttgcattc acccggggtg
    12361 ataacgcctg ggcagttcgg gccgatcata cgaacgcctg cttcgtccag tttcactttc
    12421 acggtcagca tatccagcgt cgggatgcct tcagtgatgg tgataatcag tttgatgcct
    12481 gcatcgatag cttccagaat ggagtctttg cagaacggtg ctggtacgta aataacagac
    12541 gcggaagcgc cagtagcagc aacagcttca cgcacggtgt tgaacaccgg caggccgagg
    12601 tgggtagtgc cgcctttacc tggagttacg ccaccaacca ttttagtgcc gtatgcaatg
    12661 gcctgttctg agtggaaagt cccctggcta ccggtaaagc cctggcagat aaccttggtg
    12721 tttttatcaa ttaaaatgga cattatttcc cctccactgc ggcaacaacc tgctgagctg
    12781 catccgtcag accttttgct gcaataatat tcaggccgct gtcagccagt ttcttcgcgc
    12841 cgagttcggc gttgttacct tccagacgta ccacgaccgg tacgttaaca cccacttccg
    12901 ctaccgcgcc gatgataccg tcagcgatca ggtcgcaacg aacgataccg ccgaagatgt
    12961 taaccagaac ggctttcact ttgtcgtcag agaggatgat tttgaacgct tcggttacac
    13021 gttctttggt tgcgccgccg ccaacgtcca ggaagttagc cggttcgccg ccgtgcagtt
    13081 taactatgtc catcgtaccc atcgccagac ctgcgccgtt aaccatacaa ccgatgttac
    13141 cgtccagcgc aacgtagttc agttcccact gtgcagcctg tgcttcacgc ggatcttcct
    13201 gcgactggtc acgcatttcg cgcagatcag gctggcggaa cagggcgttg ccatcagcgc
    13261 ccagtttgcc gtcgaggcaa atcagatcgc cctgtttggt gatgaccagc gggttgattt
    13321 cgatcagcgc caggtcgcgc tccaggaaaa tggtcgccag gcccatgaag attttggtga
    13381 actgctgaac cagtttacct tccagaccca gtttgaacgc cagctcgcgt ccctgatacg
    13441 gcatcgggcc agtcagcgga tcaagcgcaa ctttatggat caggtgcgga gtttcttccg
    13501 ccactttttc gatttccacg ccgccttcgg tggaggccat aaagaccacg cgacgggaac
    13561 tacggtctac aacggcaccg agatacagct ctttagcgat atcggtcgcc gcttcaacca
    13621 gaatctggtt aaccggttgg ccattggcat ctgtttgata cgttaccaga cgcttgccca
    13681 gccagttttc tgcaaaagca cggatgtctt ctttgctgtt tacaactttc acaccgcccg
    13741 ctttaccgcg gccaccagcg tgaacctgac atttcactac ccacggaccg gcaccgattt
    13801 ttgaagcggc ttcttctgct tcgcgcggag tagtacaggc ataacccacc ggtgctggta
    13861 agccatagcg ggcaaacagt tgttttgcct gatattcatg taagttcatg tgttctgtcc
    13921 atccttcagt aatcgttatc ttttaaaccg tagaccgaca ccctatccac gtcggtctac
    13981 agtgcaggtg aagcttaaac tactacacgt ccagcagcag acgcgtcggc tcttccagta
    14041 actctttgat cgttaccagg aagcccacgg attcgcgacc atcgatcaga cggtgatcgt
    14101 aggacagcgc caggtacatc atcggcagga tctcaacctg accattcacc gccatcggac
    14161 gatctttgat agcgtgcata cccagaattg cgctctgcgg cgggttgatg atcggcgtag
    14221 acatcaggga accgaacaca ccaccgttgg tgatggtgaa gttaccaccg gtcagatctt
    14281 caaccgtcag cttgccatca cggcctttga ctgccagctc tttgattttc ttctcgatgt
    14341 ctgccatgcc gagggtatcg acatcacgca gaaccggcgt caccaggccg cgcggcgtag
    14401 aaaccgccat gctgacgtcg aaatagttgt ggtaaaccac gtcatcgccg tcgatagacg
    14461 cgttcacttc cgggtaacgt ttcagggctt caaccaccgc tttcacgtag aaggacataa
    14521 agcccagacg gatgccgtgg cgtttttcaa acgcttcacc gtactgctta cgcagatcca
    14581 taatcggctt catgttgact tcgttgaacg tggtcagcat ggcggtggag tttttcgctt
    14641 ccagcagacg ctctgccaca cgcttacgca ggcgagtcat cgggacacgt ttttcactac
    14701 gtgcagccag agccggttgc gccgccggag cagccgctgc cggagcagac tctttcgccg
    14761 gggctttcgc cagatgtttt tccacatctt cacgagtcag acgaccaccc acaccggtgc
    14821 ctttaatggc gctggcgtcg agattgtgtt cagccagcag gcgacggatc gccgggctta
    14881 acgcatcgtt gttttgctct tccagagacg cctgctggcg ttgcgccgga gtggacgctt
    14941 tctcttcaga tttggcgctg gtttctttac cggcgctgtt gccttcacgc aggcgaccaa
    15001 ggatctgacg agacgttacc gttgtacctt catcttccag aaccgcatcc agaatgccgt
    15061 ctgctgatgc cggtacttcc agtaccactt tgtcagtttc gatttctacc agcacttcat
    15121 cacgtacgac tgcgtcgccg ggttttttat gccaggttgc gacggtggca tcggctacgg
    15181 attcaggcag gtcagggacc agaatatcta cgctactcat tgtgtatcct ttatttattc
    15241 gacgttcagc gcgtcattaa ccagatcttg ttgctgtttc tggtgaacgg acatataccc
    15301 taccgccgga gaggcggagg ccgggcggcc tgcataacgc agagaagccc caaacggaat
    15361 cacttcacgg aaatgatgct ggctgcagta ccatgcgccc tggttgagcg gctcttcctg
    15421 gcaccagaca aaatccttga cgtgagcaaa ctgctgcaac acttcctgca tcgctttatg
    15481 cgggaacggg tagagttgct cgatacgcac aatggcgaca tcgtgttgat tgttcttacg
    15541 acgctgttcc agcaggtcgt aataaacctt accagaacac atcactacgc gcttcacgcc
    15601 cttcggatca agctcgtcga tttcaccgat ggctggcagg aaggtgccgt tcgccagttc
    15661 ttcgaggctg gaaaccgcca gcggatgacg cagcagggat ttcggcgaca tcacgaccag
    15721 cggacgacgc atcccgcgca gcgcctgacg acgcagcatg tggtaaacct gtgccggggt
    15781 agacggtacg caaacctgca tgttttgctc agcacaaagt tgcagataac gttccagacg
    15841 cgcggaggag tgctccggcc cctgcccttc gtaaccgtgc ggcagcaaca tcaccagacc
    15901 acacatccgg ccccatttct gctcgccaga ggagatgaac tggtcgataa ccacctgcgc
    15961 accgttggcg aagtcaccga actgcgcttc ccagatggtc agggtgcgtg gttctgcggt
    16021 ggcataacca tattcaaacg ccagcacggc ttcttctgac agtacggagt cccagacacg
    16081 gaacgcgccc tgaccgttat ggatgtgttg cagcggcgtg taagtggaac cgttagactg
    16141 gttgtggatc accgcgtggc ggtggaagaa ggtaccgcga ccggagtctt cacccgacag
    16201 gcgaaccgga atgccttcat caaccagcgt ggcgtaagcg aggttttccg caccgcccca
    16261 gtcgaacagt ttctcaccgg ctgccatcgc ctggcgatcg ccataaatct tggcaacgcg
    16321 agactgcatt tcaactgctt ctggtaccgt actgatacgt ttagccagtt cctgcaggcg
    16381 cttcatctca actttgttcg ggtactcttc gtcccattcg tggttgaggt acggcgacca
    16441 ggtgaaagag tgcatgttca tcggacgcca ctctgccact acgcaatcgc cagcatccag
    16501 cgcatcgcgg tacaggttaa ccatctcggt ggcatcttcc agcgtcgcca ctttttcctg
    16561 ctccagcttg tcagcgtaga ttttgcgcgg tgtcggatgt tttttgattt tctgatacat
    16621 cagtggctgg gttgcgctcg gctcgtcggc ttcgttgtgg ccgtgacggc ggtagcatac
    16681 caggtcgatg aagacatcac gtttaaaggt gttacggaaa tcgagcgcca gacgggtcac
    16741 aaaggcaacg gcttccggat catccgcgtt aacgtggaaa atcggtgcct gaaccatctt
    16801 accgatatca gtacagtacg gtgtagaacg ggcatccagc gggttagagg tggtgaaacc
    16861 aacctggttg ttgataacga tacgtaccgt accgccaact tcataaccac gcgctttcga
    16921 catgttcaga gtttcctgaa ccacgccctg cccggtcact gcggcgtcac cgtggatggt
    16981 gattggcagc actttgttgc tgttcggctc atcaagtctg tccagacggg cacgaacaga
    17041 gccgataacg accgggctta caatctcaag gtgagacggg ttaaacgcca gcgccaggtg
    17101 caccaggccg ccatcggtct ggaagtcaga cgagaagccc atgtggtatt tcacgtcgcc
    17161 tgtgccgagg tgttctttat gtttaccggc gaactcgtcg aacaagtctt gcggtttttt
    17221 acccagcacg ttcaccagca cgttcagacg accacggtgc gccatcccga gaaccacttc
    17281 gcgggtgccg ctgttgccag cgtggcggat catctcttta agcatcggga ttaacgcgtc
    17341 accgccttcc agcgagaagc gttttgcgcc agggaatttt gcgccgaggt aacgctcaag
    17401 gccttcagcg gcggtcagtt cgcttaagaa gcgttttttc tcttcgctat tgaaagtcgc
    17461 gcgaccagac tcgatacgct gttggatcca gcgtttttct tcggtgctgg tgatgtgcat
    17521 atactcggca ccaatcgggc cgcagtaggt ttgcttgagg gcggaaataa gctcgccgag
    17581 tttcatggtt tctttgccgc tggcaaatga accgacgttg aaggtttcct ggaagtctgc
    17641 ttcggtcaga tcgtggaaag acggatccag atcggccact ttatcttgct gccacagtcc
    17701 cagcggatct agattcgcat gctggtgacc acggaagcgg tatgcgttaa tgagctgcag
    17761 gactttaacc tgcttcacat tggtgtcagg gtcggagatc gttgaagagt aacgtgaagc
    17821 gtctttcgcc aggcggcgga aatattcacg cgtttgagag tggaattgat ccggtttgac
    17881 tcccgtacca ggtaactgct ggaacgtcga acgccagtta gcgtcaaccg agtcaggatc
    17941 ggttaagaag tcttcataga gctgttctat ccagctctgg tttgcgccag agaggtaaga
    18001 agagtccaac caggctttca aagcgctgtt ctgcatcgtg atcccttaag catctttttt
    18061 atgcttactt cgccgtggat actaccacgc acagtgatgt gcgtgccggg gttcacttgc
    18121 gagctcttgt ctacgtagtg gctcgcgaag gaacctttaa aaactgtctt agtgtcaggg
    18181 gcagttttta aagatttcct gcatttcttt gttacgcctg atgcgctgcg cttatcaggc
    18241 ctgcgggtaa tgacttgtag gccggataag gcgttcacgc cgcatccggc actggttgcc
    18301 tgatgcaacg cttgcgcgtc ttatcaggcc tacagtttac gcattacgtt gcaacaacat
    18361 cgacttgata tggccgatgg cgcgcgtcgg gttcagcccc ttcggacata cactgacgca
    18421 gttcatgatg ctgtgacagc ggaatacgct gaatgcatcg ctcaaaccgt cgaggcggct
    18481 gtcagtctcg gtatcacggc tatcgatcag gaaacgatat gccgctaaca agcctgccgg
    18541 gccgataaac ttatcgggat tccaccagaa agacggacaa gaggttgaac aacatgcgca
    18601 gagaatacat tcatacagcc cgtcgagttt ttcgcgctgc tctggcatct gtaaatgctc
    18661 gcgagctggc ggattttgtc cattattcaa caggtaaggc ttaattttct catattgcgc
    18721 atagaattgt cccatgtcta ccaccaaatc gcggatcacc ggtaaacctg gcagcgggcg
    18781 aatcacaatc ttcttgcccg gctggttgag tgccgaaatc ggggtaatac aggccagacc
    18841 attcttgccg ttcatgttca gaccgtcgga accgcacaca ccttcacggc aggagcggcg
    18901 gaacgacagg ctgggatctt tctctttcag ctggataagc gcatccagca gcatcatgtc
    18961 gcgaccttct tccgcttcca gggtgtaatc ctgcatacgc ggagcatcat caacatccgg
    19021 gttatagcga taaattgaaa actcgagtct cattttcctg tctccgcatt agtaagtacg
    19081 aatcttcggc gggaatgccg ggcgcagttt cggttccatg ttgacgcttc ggcgcgtcat
    19141 ggattccgac tcaggcagat acagggagtg gcacagccag ttttcatcat cacgatccgg
    19201 gaagtcgaag cggctatgtg cgccacggct ttcggtacgg aagttggcag aaacagccgt
    19261 tgcatacgcc gtttccatca ggttatccag ttccaggcac tcaacgcgct gggtgttgaa
    19321 ctcgctggaa gtgtcatcca gacgggcatt tttcagacgc tcgcggatca ctttcaactg
    19381 ctcaagccct ttcgccatcg catcaccttc acggaagacc gagaagttat gctgcataca
    19441 ttcttgcagc gctttacgga tcgccaccgg atcttcaccg ttacgattat tgttccaccg
    19501 gttcaggcga tccagagacg cttcaacatc agactcgctg gcatcgtgca gtgcgccctg
    19561 ctcggcgata gactcttgca gatgcagacc tgccgcgcga ccaaagacca ccaggtccag
    19621 cagcgagttg ccgcccagac ggttagcgcc gtgtaccgat acacaagcga tttcaccaac
    19681 ggcaaacagt cccggaacaa ccacatcttc gcctttctca ttcacagtca gcgcctgacc
    19741 ggtaactttg gtcggaatac cgcccatcat gtagtgacag gttgggataa ccggaatcgg
    19801 ctctttcacc ggatcgacgt gagcgaaggt acgggaaagc tccaggatac ccggcagacg
    19861 ggattcgaga acttctttac ccaggtgatc gagtttcagt ttcgcgtgtg gcccccacgg
    19921 accatcacag ccgcgacctt cacggatttc aatcatgatg gaacgcgcca ccacatcacg
    19981 gcccgccagg tctttggcgt tcggcgcata acgctccata aagcgttcgc catgtttgtt
    20041 cagcagataa ccgccttcac cacggcaacc ttcggtgacc agtacgcccg caccggcaat
    20101 gccggtcggg tggaattgcc acatttccat atcctgcacc ggtacgcctg cgcggatagc
    20161 catgccgaca ccgtcgccag tgttaatgtg ggcgttagtg gtggactgat aaatacgccc
    20221 tgctccgcca gtcgccagca cggtagcgcg ggctttgaaa taaaccactt caccggtttc
    20281 gatgcacagt gcggtacaac ccaccaccgc gccatcctgg tttttcacca gatccagcgc
    20341 ataccactcg gagaaaatgg tggtgtggtt tttcaggttc tgctgataaa gcgtgtgcaa
    20401 cagtgcgtga ccggtacggt cagcagccgc cgcagtgcgt gccgcctgct cgccgccgaa
    20461 gtttttcgac tgaccgccaa acggacgttg atagatacga ccatcatcga gacgcgagaa
    20521 cggcaggccc atgtgttcga gttccagaat cgcttccggc ccggttttac acatatattc
    20581 aatcgcgtcc tggtcaccga tatagtccga cccttttacg gtgtcgtaca tatgccattc
    20641 ccagttatct tcatgggtat tacccagcgc aacggtaatg ccgccttgcg cagaaacggt
    20701 atgggaacgg gtcgggaaga ctttagagag cagggcacag gtctggccgc tctgggaaat
    20761 ttgcagcgcc gcgcgcatac ctgcgccacc ggcaccaatc acaactgcat caaattctct
    20821 gactggcaat ttcatcacac accccacacc acaacgaatc cataaatcac gtaaaccacc
    20881 agtgcaacga caatcaccag ttgcagcatc aggcgcaagg ccagcggttt aacgtagtcg
    20941 gtcaacacct gccacatgcc gatccaggca tgaatcaaga tagaaaacag cgccagcagg
    21001 gtgaacactt tggtgaacgc agaggcgaag aaaccgatcc agacttcata tgtcagttcg
    21061 ccactggtgg cgaaaaaacc gaccatataa atgatgtaga gcgtcaggac gatagcggta
    21121 gcgcgaacga ggatgaaatc atgtacgcca ttgcgtccta atgcggaggc gttgcttacc
    21181 atacgaggac tcctgcgaga agtgaaagca cgacagtaat aacaaaggag attttggcgg
    21241 agcgtttacc cgcttcgaat gtttcttcca gatagccaaa atccatcatc atgtggcgaa
    21301 tacctacgac gacgtgatac gccagagcgg taaggatgcc ccacatgata aatttgacga
    21361 agaagctgcc cataatcgcg gaagcttgct cgaaaccttc aggggaagag aggctggtac
    21421 ccagaagcca ctgcaggatg cccactgcaa caaaggtgat cacaccggaa acgcgatgga
    21481 gaatggacgc tatcgccgtg atggggaacc ggatggtctg taggtccaga ttaacaggtc
    21541 tttgtttttt cacatttctt atcatgaata acgcccacat gctgttctta ttattccctg
    21601 gggactacgg gcacagaggt taactttctg ttacctggag acgtcgggat ttccttcctc
    21661 cggtctgctt gcgggtcaga cagcgtcctt tccataactg cgcgtcatgc aaaacactgc
    21721 ttccagatgc gaaaacgaca cgttacaacg ctgggtggct cgggattgca gggtgttccg
    21781 gagacctggc ggcagtatag gctgttcaca aaatcattac aattaaccta catatagttt
    21841 gtcgggtttt atcctgaaca gtgatccagg tcacgataac aacatttatt taatttttaa
    21901 tcatctaatt tgacaatcat tcaacaaagt tgttacaaac attaccagga aaagcatata
    21961 atgcgtaaaa gttatgaagt cggtatttca cctaagatta acttatgtaa cagtgtggaa
    22021 gtattgacca attcattcgg gacagttatt agtggtagac aagtttaata attcggattg
    22081 ctaagtactt gattcgccat ttattcgtca tcaatggatt ctttacctgc aagcgcccag
    22141 agctctgtac ccaggttttc ccctctttca cagagcggcg agccaaataa caaacgggta
    22201 aagccaggtt gatgtgcgaa ggcaaattta agttccggca gtcttacgca ataaggcgct
    22261 aaggagacct taaatggctg atacaaaagc aaaactcaac ctcaacgggg acacagctgt
    22321 tgaactggat gtgctgaaag gcacgctggg tcaagatgtt attgatatcc gtactctcgg
    22381 ttcaaaaggt gtgttcacct ttgacccagg cttcacttca accgcatcct gcgaatctaa
    22441 aattactttt attgatggtg atgaaggtat tttgctgcac cgcggtttcc cgatcgatca
    22501 gctggcgacc gattctaact acctagaagt ttgttacatc ctgctgaatg gtgaaaaacc
    22561 gactcaggaa cagtatgacg aatttaaaac tacggtgacc cgtcatacca tgatccacga
    22621 gcagattacc cgtctgttcc acgctttccg tcgcgactca catccaatgg cagtcatgtg
    22681 tggtattacc ggcgcgctgg cggcgttcta tcacgactcg ctggatgtta acaatcctcg
    22741 tcatcgtgaa attgccgcgt tccgcctgct gtcgaaaatg ccgaccatgg ccgcgatgtg
    22801 ttacaagtat tccattggtc agccatttgt ttatccgcgc aacgatctct cctatgccgg
    22861 taacttcctg aatatgatgt tctccacgcc gtgcgaaccg tatgaagtta atccgattct
    22921 ggaacgtgct atggaccgta ttctgatcct gcacgctgac catgaacaga acgcctctac
    22981 ctccaccgtg cgtaccgctg gctcttcggg ggcgaacccg tttgcctgta tcgcagcagg
    23041 tattgcttca ctgtggggac ctgcgcacgg tggtgctaac gaagcggcgc tgaaaatgct
    23101 ggaagaaatt agctccgtta aacacattcc ggaatttgtt cgtcgtgcga aagataaaaa
    23161 tgattctttc cgcctgatgg gcttcggtca ccgcgtgtac aaaaattacg acccgcgcgc
    23221 caccgtaatg cgtgaaacct gccatgaagt tctgaaagag ctgggcacca aagatgacct
    23281 gctggaagtg gctatggagc tggaaaacat cgcgctgaac gacccgtact ttatcgagaa
    23341 gaaactgtac ccgaacgtcg atttctactc tggtatcatc ctgaaagcga tgggtattcc
    23401 gtcttccatg ttcaccgtca ttttcgcaat ggcacgtacc gttggctgga tcgcccactg
    23461 gagcgaaatg cacagttacg gtatgaagat tgcccgtccg cgtcagctgt atacaggata
    23521 tgaaaaacgc gactttaaaa gcgatatcaa gcgttaatgg ttgattgcta agttgtaaat
    23581 attttaaccc gccgttcaaa tggcgggtta atttttatat gcctaaacac aaaaaattgt
    23641 aaaaataaaa tccactatcg gacattcata tattctaaaa aaaaatacat cggtataaat
    23701 tataaacaaa tagatgattt ttaatcatct atcttcctct cagttacatg aataaaccac
    23761 caaatatagc gcacccctat aattcccaat actttattca tcaaacattt aattcatacc
    23821 atcaattaat tacttttaca tttaataaaa ttaataatat ttttaagaaa aactaaaaac
    23881 attcgacaat caaataagat attcttgcac cgatatactt ttttcttttc tgttaaagga
    23941 ctcagttatg tttaaaggac aaaaaacatt ggccgcactg gccgtatctc tgctgttcac
    24001 agcacctgtt tatgcggctg atgaaggatc cggtgaaatt cactttaaag gtgaagttat
    24061 tgaagcaccg tgtgaaatac atcaggatga tattgataaa gaggttgaac tcggtcaggt
    24121 gaccaccagc cacattaatc agtcacatca cagcgatgcc gttgctgtcg acctgctctt
    24181 agtcaactgt gatctggaaa actccagcaa cggttccggt ggcaagattt ctaaagttgc
    24241 agtcaccttt gatagctcag cgaaaaccac cggcgcagat ccgattctca acaacaccag
    24301 cacgggtgaa gccaccggcg tcggcgtacg tttaatgaat aaagatcaaa gcaatatcgt
    24361 tttaggcacc gctactccag atatcgacct ggctccgacc tccagcgaac aaacgctgaa
    24421 tttctttgcc tggatggaac aaattgatca ggctacacct gtaacgccag gcgcagttac
    24481 ggcgaatgca acctacgtgc tggattataa ataagtatta ctttggcggc gctatcgccg
    24541 cctttcataa aatatttatc aggtatggac accgtgaata tttatcgact ctcttttgta
    24601 tcctgcctgg tcgtggcgat gccttgcgca ttggcggtcg aattcaatct gaatgttctc
    24661 gataaatcaa tgcgcgaccg cattgatatt tcattattaa aggaaaaagg agtcattgct
    24721 cccggtgagt attttgttag cgttgcggta aataataacc aaatcagtaa cgagcaaaag
    24781 attaactggc acaaaaatga cgataaaacc attccgtgca tcaatgattt actggtcgat
    24841 aaatttggct taaaacctga agtccgtcag tcgttaccat tgataaatca gtgcgtcgat
    24901 tttagctccc gacctgaaat gctcttcaat ttcgatcaag ccaatcagca actgaatatt
    24961 agtattccgc aagcctggct ggcgtggcac tcagaaaact ggaccccacc ctccacatgg
    25021 aaagaaggtg tcgccggtgt cctgatggat tacaacttgt ttgccagcag ctaccgccca
    25081 caggatggca gcagcagcac taatcttaac gcctatggaa cagccggaat caacacgggg
    25141 gcatggcgct tacgcagtga ttatcagttg aatcatactg atagcgatga taaccatgaa
    25201 cagtcaggcg aaatatcacg tacctatctt tttcgcccgt taccgcaatt aggttcaaaa
    25261 ttaaccctcg gcgaaacgga ttttagtccc aatattttcg acggtttttc ttataccggc
    25321 gcggcactgg caagtgacga tcgaatgttg ccatgggaac tacgcggcta cgccccacaa
    25381 attagcggta ttgcacagac caatgccacg gtgacgatca gtcaatcagg ccgcgtcatt
    25441 taccagaaaa aagtcccacc aggcccattt atcattgacg accttaatca gtctgttcag
    25501 ggcacactgg atgtcaaagt gacggaagaa gatggtcggg tgaacaattt ccaggtttca
    25561 gcagcatcga cgcccttcct gactcgtcag ggacaggttc gctataaact ggccgcgggt
    25621 cagccacggc cctccatgtc acatcaaact gaaaatgaaa ccttttttag caatgaagtt
    25681 tcctggggga tgctgtcaaa cacctcgctg tacagcggcc tgctgctttc tggtgatgac
    25741 taccattctg ccgcaatggg tattgggcaa aatatgctgt ggcttggtgc gctgtcgttt
    25801 gatgtcacgt gggccagtag ccattttgat actcagcagg acgagcgggg cttaagctac
    25861 cgttttaatt acagcaaaca agtggatgcc actaacagca cgatttccct cgccgcttat
    25921 cgtttctccg atcgtcattt tcacagctac gccaactatc tggatcacaa atacaacgac
    25981 agcgatgcgc aggacgaaaa acagacgatc agcttatctg tgggccaacc gattacccca
    26041 ctaaacctca atctttacgc caacctgcta catcaaacct ggtggaatgc agacgcctcc
    26101 acgaccgcca acatcacggc aggttttaat gttgatattg gtgactggag agatatctct
    26161 atttctacgt cattcaatac aacccattac gaagataaag atcgcgacaa ccagatttac
    26221 ctgtcgattt cgctcccctt cggtaacggt ggtcgggttg gctatgacat gcaaaacagt
    26281 agccacagca ccacacaccg catgtcgtgg aacgatacgc tggatgaacg taatagctgg
    26341 ggcatgtctg ccggactgca atccgaccgt cctgacaatg gagcccaggt gagcggtaac
    26401 tatcagcacc tgagttcagc gggtgagtgg gatatttctg gtacctatgc cgccaatgat
    26461 tacagttccg tcagcagcag ctggagcggt tctttcaccg caacccaata tggtgcagcg
    26521 tttcatcgcc gcagctccac caatgaacca cgcctgatgg tcagcaccga tggcgtggca
    26581 gatattccgg ttcagggcaa tctcgactac accaaccatt ttggcattgc ggtggtgccg
    26641 ttgatttcca gttaccagcc ttccaccgtg gcggtgaaca tgaatgactt acccgacggc
    26701 gtaacagttg cagaaaacgt tatcaaagaa acgtggattg aaggcgcgat aggttacaaa
    26761 tcactggctt cccgttccgg taaagacgtt aacgtcatca ttcgcaacgc cagcggtcag
    26821 tttcctcccc tcggagcgga tatccgccag gatgacagcg gcattagcgt ggggatggtt
    26881 ggcgaggaag gacatgcctg gttaagcgga gtcgctgaaa atcaaaagtt taccgtggtc
    26941 tggggtgata gccagcattg ctcgctccat cttcctgaac atatggaaga caccgcaaat
    27001 cgcctgattt taccttgtca ttaattaagg aaatgaaaat gacatttatg aaaggactac
    27061 cccttttgtt gttggttgcc agtctgtgca gccacgctgc actacaaccc gatcgcactc
    27121 gtattgtgtt taacgctaat gataaagcta ccagtctgcg ggtggataat cgtagcgata
    27181 aactccccta cctcgcttat tcctggctcg aaaatgaaaa aggggagaaa agcgatgatc
    27241 ttctggtcgc cctgccgccg atacaacgtc tggaaccgaa agcaacaaca caggtacgga
    27301 tagtaaagca agcctcgacc acaaaattac cgggcgatcg cgaaacgcta tttttctaca
    27361 atatgcgtga aattcctccc gccccggaaa aaaacagcga ccatgccgtt ctgcaagtcg
    27421 ccattcagag tcgtatcaag gtgttctggc gaccggctgc attacgcaag aaagcaggag
    27481 aaaaggtcga actgcagtta caggtcagcc agcagggcaa ccaactgaca ctgaaaaatc
    27541 ctaccgcgta ttatctgacc attgcctacc tgggacgaaa tgaaaaaggc gttctccccg
    27601 gcttcaaaac cgtgatggtt gcgcctttta gtacggtcaa cacaaacacc ggaaattata
    27661 gcggcagcca gttttatctc ggttatatgg atgattacgg cgcgttacgc atgacgacgc
    27721 tcaactgcag cggacaatgc catttacaag ccgtggaggc gaagaaatga gtgctggcaa
    27781 gggagtgttg ctcgtcatct gtctgttatt tctgtcgttg aagtctgcgc ttgccctgaa
    27841 ctgctacttt ggttcatccg gtggctcggt agaaaaatca gaagcgattc aaccttttgc
    27901 tgtaccaggc aatgccaaac tcggcgataa gatctgggaa tctgacgata ttaaaataag
    27961 atctgggaat ctgacgatat taaaattccc gtctattgtg acaacaatac caacggcaac
    28021 tttgaaagcg aacatgtcta cgtatgggta aacccctacc cgggggtgca ggatcgttat
    28081 tatcaactcg gagtaactta caatggcgtt gattatgacg ccaaccaggg aaaaagccgc
    28141 atcgacacta accagtgtat cgacagtaag aacattgaca tttatacccc tgatgggatg
    28201 gcagaacaaa atttgctcgg gtgatcccgc taatattcac atgtcgcgaa cctttctcgc
    28261 tcgcatgcgg ttatacgtca aaatacgaga aatgccgccg catgattatc aaagtacgct
    28321 tagcgactat atcgtcgtgc aatttgacgg tgccggtagc gttaacgaag accctactgc
    28381 ccaaaacctg aaatatcata ttactggtct ggaaaacatt cgcgtgctgg attgcagcgt
    28441 caatttttcc atttcaccgg aaacacagat gattgatttt ggtaaattta atttgctgga
    28501 tattcgtcgc cacacaatgt cgaaaacatt cagtattaag acgacaaaat cacaaaatga
    28561 ccaatgcacc gacggattta aagtcagttc ctccttttat accgaagaaa cgttggttga
    28621 agaagataaa gcattgttga ttggtaatgg tctgaagcta cgtttgctgg atgaaaacgc
    28681 ctcgccttat accttcaata aatatgctga atacgccgat ttcaccagcg acatgttggt
    28741 ctacgaaaaa acctatacag ctgaactttc gtccattgca ggtaccccca tcgaggctgg
    28801 gcccttcgat acagtggtgc tttttaaaat taactataac tgatttgcca tgatgccctt
    28861 tgcagccgga aagggcatct ctttcagaaa attgctgatt ttcccctttt ccttgattgc
    28921 tgttattttt tcctagcccg gctttcttta tgctgtatcg atacaccaaa gagaaaataa
    28981 tgagggagcg aaggatgcca gttttgcagt ggggaatgtt atgtgtgcta tcacttctcc
    29041 tttctattgg tttcctcgcg gtacatctcc cggcggcgct attacttggg ccgatgatcg
    29101 ccgggatcat cttcagtatg cgcggaatta ctctgcaact tccccgctcc gcttttctcg
    29161 ccgcgcaggc cattcttggc tgcatgattg cgcaaaacct cactggttcg attctcacca
    29221 cgctggccgc caactggccg atcgtgcttg ctattttact ggtgacgctg ctttccagcg
    29281 ccatcgtggg ttggttattg gtgcgctata gctcactgcc tggaaatacc ggtgcctggg
    29341 gttcttctcc aggcggcgca gcggcaatgg tcgctatggc gcaagattac ggcgcagata
    29401 ttcgcctggt ggcgtttatg caatatctgc gagtgctgtt cgtcgcgggg gccgctgttc
    29461 tggtcacccg catgatgctg ggtgataacg ctgaagcggt taaccagcaa atcgtctggt
    29521 tcccgccagt gagcattaat ctcctgctta cgattttgct cgcagtcgtt gccggtacgg
    29581 cgggatgcat gctgcgtctt ccttccggca cgatgctcat cccgatgctg gcgggcgcag
    29641 tgctccagtc tggtcagctc atcaccatcg aactaccgga atggctgctg gcgatggcgt
    29701 atatggcaat tggctggcgg attggtcttg gtttcgataa acaaatctta ctgcgggcac
    29761 tacgcccgct accgcaaatc ctgctgtcga tttttgctct gctggctatt tgtgcaggta
    29821 tggcgtgggg actgacccgg tttatgcata ttgattttat gactgcctac ctcgccacca
    29881 gtcccggcgg gcttgatacc gtggcggtca tcgccgcagg gagcaatgcc gatatggcgc
    29941 ttattatggc gatgcaaacc ctgcgcctgt tcagtatttt gctgacgggg ccagccattg
    30001 cgcggtttat ttcaacctat gcgccgaagc ggtcggccta gtgctggcag cctggacacc
    30061 agtaaaacgg gcgcgaggac aacgtggttt tctcaatgat gccgccacag cgttcgcaca
    30121 gttcgccatc acgatgaaaa accttaaagc gaaacagcgc cccatgatgc ttattctcat
    30181 ccacctgccc ccgcgtagcg taagaaaacc gaggaatctc cagtaacgcg tgtgcgagtg
    30241 catccagttg cgccgcattg agatctttcg ctttatgatt tccagtcaac ccaacctgcc
    30301 agaggatctc cacccgcaaa taattgccaa gcccagccag aaacgcctga tcgagcagta
    30361 atcctgcaaa ctgacggtta cgaaagcgcg gcgacaataa ccgttccttt accacctccg
    30421 gcgtcagatt cggatccagc acatcgggac caacgcgttg taaaaacgga tgcgtggtca
    30481 gttgttccgg cctcaacatc tcaatatccg aggcgctata aagcagaatg gttttgtcag
    30541 ccgtttgcag ttttacccgc aatactcgcg tggtctgcgg ctcttcgccg gtatcaacca
    30601 cgcgccagac gccgtaaagc tgattatggc tgtagagcgt taaaccgttg ggaaaatgag
    30661 ttaacaacgc cttaccacgc gtttccacat gggtaacgtg ttgaccgata agttgtgatt
    30721 gataagtttt taactgcggg aaggcaaacc agacatcagt tagtggtttg cctttgatcg
    30781 ccgcctccag gttatccgct gcacggcgga tctccgggcc ttcaggcatg gtgatatcct
    30841 tttttcattc tttattaagc ggggttatgc tgcgacaaca atcccctttt tggcaaatgt
    30901 agctcgtaat cggcgggcga acgccagagc gtgctcgccg tcgccatgca ggcagaccgt
    30961 ttgcgccgcg accgttgccc attcgccggt gatacttttg actctgccgt gttgcaccat
    31021 ctccagcgtt tgcgccaacg cctgttcttc gttttcaatc aacgcgcctg gctgacttcg
    31081 cggcaccagc gagccgtcag cctgataacc gcgatcggca aacacttcct cgcgcgttgt
    31141 caggccatat tgcttgcctg cacgaatcag ctcgcttcct gccagcccaa cgagaaccaa
    31201 tgccggatcg caagcgtata ccgctctggc gatggcgtct gccagttgtg cttctttcgc
    31261 cgcctggttg tacaacatgc cgtgcggttt gacatgacgc attacgccgc cttgcgcacg
    31321 ggcaatcgtt gccagcgcgc caatttgata cagcgtctgg gcgtaaacgg tttccggcgg
    31381 cagctgcatg gcgctgcgac caaaattttc cctgtcggga aaactcgggt gagcgccaat
    31441 cgcgacacca ttttttattg cttcacgcac gcaagcctgc atggtttgcg catcgcctgc
    31501 atgaaatcca caagcaatat tggcagagga aaccagcgtt aatagctctg cgtcgctggc
    31561 gcagccttcg cccagatcgg cgttcaggtc aattttcatt gtgcagccgc cacgctaatt
    31621 gttcaaaata acgttgctga tcttgccgcg ctttcagtgc ctcttccagt gaacactgga
    31681 caaaatgaat cggctgaccg agcggaattt gcgccagatg gtacatatca gcctcaatga
    31741 tacaggcaat acgtggataa ccaccggtgg tctgtgcgtc gttcatcaac acaatcggct
    31801 gcccgttatg cggcacctgc accacgcccg gtaacaaacc gtgagataac agttcgcgat
    31861 cggtgatgcg ttttaaaatt tgcccctgta aacgatagcc cattcggtta ctttgcgagc
    31921 taagctgcca gggcgaacgc cagaatgcat cctgcgaggc gcgatcgaac tcatgatatt
    31981 ccggccccgg caaggcgcga atgcggttgc cccacagcag ctgtttaacg ccctgcgctt
    32041 ccataaaatc acgcttcgat ttgccaatcg ggagtcagtc accatccttc agtaaacggc
    32101 cttccagccc gccaatcccc actttgagat cggtgctgca tgaccccatt accggcggaa
    32161 catcaatacc acccgcgacc gccagataac tgcgcatccc gtgctgcggg cgtttaagcg
    32221 ttaaacgctg gcctgctttc atcggcaatc gccagccggt ccagacggcg ttatcatcca
    32281 accgcgcttc gcaacctgca cccgtcaggg caaaccaccc atcagtttcg aactcaactg
    32341 ttaactgacc gagcgtgatc tccagcgcgg gggcattggc gtcattaccc accagcaggt
    32401 tagcaatgcg cagcgcaggc atatccagtg cgccacagtg gctgataccc gactggcaaa
    32461 aaccgtgacg accgccatcc tgcacagtgg tgtacatgcc cgcacgaata atcttcagca
    32521 tattccctcc ttctgcggta caaatcgcac gctgtctccc ggacgtaata agatgggttc
    32581 gtcacgcgcc ggatcaaaca ggctgagtga ggtatgacca atcaactgcc agccacccgg
    32641 cgttgccagc ggataaacac cagtctgcgg cccgccgatc ccgacagaac ctgccggaac
    32701 gagcaagcgc ggttcagcgc gccgtggcgt gtgtagttgt tccggcaaac tcccgagata
    32761 cgggaagccc ggttgaaaac ctaaaaacca gaccacgtat tccacggagg agtgcaattc
    32821 aacaacctgt ttttcgctca acccgcaata cgccgcgacc accgccaaat ccggtccgcc
    32881 tgcaccaccg taaaccaccg gaatttcaat aaagcgagac tcaggttcca gggcctcgct
    32941 ctcctcccac cagcgttgca aacgctctat ggcatccagc gccagcgact caggattacg
    33001 caaaatcacc gtgatattgt tcatgccggg aatggcttca accacattcg gcatatccac
    33061 cagacgctgc gccagtcgcc agatccgttt ctggctagcc agcgtcaccg gcggttccag
    33121 ttccagcact accgccgttt cacctatcag ataacaacgc gctcgttgca ctttcgatcc
    33181 ctctgattat tcgttatgca ggattaggaa tatcaataaa ggtcacatca agatcggtat
    33241 tttcattcag ccactcgctc aatgcgcgaa taccaccgcg ttcagtggca tggtgacctg
    33301 cagcataaaa atgcaatccc tgctcgcggg ctgaatgaat ggtctgttca gaaacttcgc
    33361 cagtaataaa agcatccacg ccaaaacgcg cggcgctatc gataaaactt tgcccgccgc
    33421 ccgtgcacca ggcgacgcgc tggaccacct caggtccggt atcgccacac cataacggct
    33481 tacgtcccag acgcgcttca atccaggagg ctagttccat tcccggcaca ggcatggtca
    33541 gctctcccca cggcaccaac ggctcaattt cgcccatgac cgtgatcccc agtaacgccg
    33601 ccagctgtgc gttattgccc agctcaggat gtgcgtcaag cggcaaatgc cagccataca
    33661 ggttgatatc attcgccagc aacgttttta aacggttacg cttcattccg cgaatgaccg
    33721 gagactctcc tttccagaag tagccatgat gcacaatgac cgcatccgcg cccagacgca
    33781 ctgcctcatc gagcaaagcc tggctggcgg tgacaccggt aacaattttt tgcaccgtct
    33841 ctttgccttc cacctgcaaa ccattcggcg cgtaatcact aatcgccgcg ctgttcagtt
    33901 tttcgttaat cagttgttcc agttcggtgt ttttcatctt cgcgccttcc gtattctgat
    33961 agctacaccg ttaacatagc gttacatcgc ctgttcgtcg atagccaatc ttttgacata
    34021 ttaagaaaca aacccgacgc caaaagacgc aaattgttta tatcatcacg cttcagctaa
    34081 tgtatattta tgcaatcaga cagcatattg cataaataat cagatctgta tcaaagtttt
    34141 aactttaagg aaacacaagc acgccgcgca actattacgt gacgttgccg tcaattgtgg
    34201 aattattatg aataaacacg catcacaacc gcgcgctatt tactatgtcg ttgcgctgca
    34261 aatctgggaa tattttagct tttacggcat gcgtgccctg ctgattctct atctcaccaa
    34321 tcaactaaaa tacaacgata ctcacgccta cgagttattt agcgcctact gttcgctggt
    34381 gtatgtcacg ccaatcctcg gtggcttttt ggcggataaa gttctcggca atcgcatggc
    34441 ggtgatgctg ggggcgttgt tgatggcgat cggtcatgtg gtgctgggtg ccagtgagat
    34501 ccatccgtca ttcctctatc tgtccctggc gattatcgtc tgcggctatg gcctgtttaa
    34561 atctaacgta agctgtctgc tcggcgagct gtatgagcca accgatccgc gtcgtgatgg
    34621 cggtttctcg ctgatgtatg cggcgggtaa cgtggggtct attatcgcac ctatcgcctg
    34681 tggttacgcc caggaagagt acagctgggc gatgggcttt ggcctggcgg cggttggaat
    34741 gatcgcgggt ctggtcattt tcttatgtgg caatcgtcat ttcactcata cccgcggcgt
    34801 taacaaaaaa gtactgcgtg cgacaaactt tctcctgccg aactggggat ggctgctggt
    34861 tctgctggtg gcaacgcctg cactgattac cgtgctgttc tggaaagagt ggtcggtata
    34921 cgccttaatt gtcgcgacta ttattggcct gggtgtactg gcaaaaattt atcgcaaagc
    34981 agaaaaccag aaacagcgga aggagctggg gctgattgtg acgctcacct tcttcagtat
    35041 gttgttctgg gccttcgcac aacagggcgg tagctcgatt agcctttata tcgaccgctt
    35101 cgttaaccgc gatatgtttg gttataccgt tccgaccgcg atgttccagt cgattaatgc
    35161 cttcgcagtt atgctgtgcg gtgtgttcct ggcgtgggtg gttaaagaaa gcgtcgcagg
    35221 taatcgtacc gtgcgcatct ggggaaaatt tgctcttggc cttggcctga tgagcgccgg
    35281 attctgcatt ctgaccttaa gcgcccgctg gtccgcaatg tatggtcact cttctctgcc
    35341 actgatggtg ttaggcctcg cggtgatggg ctttgcggaa ctgtttatcg acccggttgc
    35401 catgtcgcaa attacgcgca ttgaaattcc gggcgtgacc ggcgtattaa ccggcatcta
    35461 catgctgctt tctggcgcga tcgcgaacta tctggcgggc gtgattgccg atcagacatc
    35521 gcaggcttcg tttgatgctt ccggggcgat caactactcc atcaatgcat atattgaagt
    35581 atttgatcaa attacctggg gcgcactggc gtgtgtagga ctggtactga tgatttggct
    35641 gtatcaggcg ctgaaattca gaaaccgcgc gctggcgctg gagtcataat aggccagcac
    35701 cgatgcctga tgcgacgctt gacgcgtctt atcaggccta cgtatttcct gcaatttatt
    35761 gaatttgcac aaatttgtag gctggattag gcgttcacgc cgcatccggc atctgggctc
    35821 gattgcctga cgcgtctgtt atttcccctt ccgcgccgcc tcatacgctg ccaacgtttg
    35881 tactctcgcc tctttgtgct cgactatcgg ttgcggataa tccagcttca cacctgcttt
    35941 ctgcgcccac ttccacggct catgcaccgc tttccctggt acatcgcgca gctccggtaa
    36001 ccatcgacga ataaactcgc cctcacggtc aaatttctcg ccctgagtta tcggattgaa
    36061 aatacgaaaa tacggcgcag catcagtacc cgtagaagcc gcccactgcc agccaccgtt
    36121 attagctgcc aaatcaccat caatcagctg cgacatgaaa tatcgctcgc cttcgcgcca
    36181 gtcgatcaac aaatctttca ccagaaaact ggctgtaatc atccgtagcc ggttatgcat
    36241 ccagccagtg ctgttaagct gacgcatagc ggcatcaata atcgggtatc ccgttttgcc
    36301 tttctgccag gcctgtaaat gtgcgggatt gctttgccac tgcacacgat ccgtccaggc
    36361 aataaatgga cgatgtttac acaacgaggg gtaatacgtc atcagatgac ggtagaactc
    36421 gcgccagatc agctcactaa gccagacact accggcccca ccgtccagcg cctgcggctg
    36481 ttccgccaac aagcgatgca aacactggcg aggcgataac ccgcccgttg ccaagctggc
    36541 ggacaaacgg ctggtgcctt ccactgccgg aaaatctcgt tgttgctcat actctccagc
    36601 accgttctcg caaaactggc gtaattgcgc aatcgccgct ttttcttcca ccgggaaatg
    36661 cgcagtatcg aaagactgac gaggataatt cagcgtaatg gatggcgcgg gctttatcga
    36721 tccgctacta cgaacttttg gtgcagcgac gcactccggc atcccttccc gcagccgttt
    36781 cagccaggca ttcttaaaag gcgtaaagac tttgtacatc tcgtgattgc cggtcatcac
    36841 cgcccccggc ggcaggatca cgctgtcatc aaatccttca cacaccacgt tacgcagcgc
    36901 tctttcaacg tccacatccc gcgctcgctc attcacctca tactgatagt tataaaaaag
    36961 atgagtaacg ctgttttccg cgcacacctg tttaactatt tcgacactgg cgacaaagtc
    37021 atccacttca cggaacaata aaggaatacc tttttccgcc agcgcgattt gtagcccatt
    37081 cagttgagca ttgataagtt cagcctgacg cggcgacatg ttatgcgtct cccacttgcg
    37141 tggtgtagcg atatacagcg ccagcacgcg tgcagacgaa ttgcggcagg cggcagccag
    37201 tgcgagatta tcgtgcagac gtaaatcctg gcgaaaccag gccagatggg tagtcataaa
    37261 actcctgaat ggcgcaagtt aatcacacgc ggccaaagtt cgaaataacg ctgccctgaa
    37321 agccaggcgt caggataaag cgccatatag tgtttgatac gcatcagcgg cgcaagaagt
    37381 ggttgctcgc cacggcgata actgtcgata agcgcagcca gctgctggcg ttctgtggaa
    37441 tcaatatgcg ggcgaaaata accctgaacg tgcatcaaaa cgttggtgtg atcgcgcggg
    37501 ttggcgggat gagaaagcaa aacaattacg cgttggcggt actggttgta aaaatcatcc
    37561 agattttgcc actgatgaat atcagccaca aacgggccaa gtttgcggta ctcgggctga
    37621 gaatgggcca gaaagacgag tttatagcgg ctgtgataat cgagaagcgc cccgcgcgtt
    37681 aagccatgtt ctttcagtcg atgtaactca tgaagggcga agatacggat tagggtgctg
    37741 tcatcaaatc gttgtagatt cattactgct cctgaacggt atacaggagt aagtgtaggc
    37801 gttggtaaat gatgtttgtg aagttaatcc gctagcaggg caagattacg aaagcgcgct
    37861 ccccgcaagc aactaatcaa ggtagttaat catggctgaa cacgatatta cgccagaagt
    37921 cactatttcc aaaacacagc gccgctataa agtcggctat gtcagagccc gccaccagga
    37981 ccgcaaaacc ggtatagccc ggtattattc acaacatccc agtctgcacc ttaagggcga
    38041 ctggctgaaa gaggccggat ttgataccgg acggggtgtc acggtgaaga tttcggaggg
    38101 gggcctgacc atcatcgctg acagtgacga ggtgcaggaa ctgcgggatg ggatgtttag
    38161 tgtgctgaac gaaagttaaa tattaaaaaa gctgggaaga tttaaaacct tcctagcttt
    38221 ttattctgtc ttattataat ggatactcat catcttccga tgaatttata aattgctcca
    38281 agtttacatg aagagtactt aacctagttt ttaactcatt aagtgaagcg ataatttcat
    38341 attttccaac ataatcatat gatggatcgt attcattatc tatgtcagaa gctaaaaatg
    38401 atgatattcg tagtttaaat ttagcttcat ctttattaca ttcattggca taaggtgaat
    38461 gccctatcat agcatattca tctaatctaa aaggataaga tggttttaat tcatcgatca
    38521 atgaaatttc aatccatgtt ctatatccag gcattaatgg actaatttct ttgagcaaaa
    38581 cctttccact taataatgta ttgagattgt tcattattat gtctcccacg ggaaaaatat
    38641 atgtctaaca cttccaccat gttgatgaac ttgtaaatgt ggtaaatcac catcaggatt
    38701 tcctggccca tgacctgaca ctctatatga aggttttgtt acagaaggat cttttatatc
    38761 atcccaatga tatgaacctg cttttgtttt attttcagta agatattcca ttaagttctt
    38821 agtgctgggg cctgactcac ctgtcatatt tttataatta tgacctaaat atctatccat
    38881 aaacaattgt tcagcgtcac ttctagacct aacagtaact ttggtttcac tactatctaa
    38941 taaacgattc gctttttggt tactaacagc acatggagtt aatccaagtg gatcagcata
    39001 ttttagagga ttaggtgcat aactgtagag atttaaaccg cctctcaacc ctatcgggtc
    39061 agttaccatg aaccgcccga catccggatc atagtacctg tgcagattgt agtgcagccc
    39121 cgtttccctg tcgaggtact gtccctggta gcgcaggttc tgctccaccc cgccgtggtc
    39181 cttctcctgt atcgtgttac cccagacctg ataccccgtc tcccacacga ttttcccgtc
    39241 gctgtccgtc atctcttccg gcgcgccgtt cacatccgtg tgaaaataca gtatccggtt
    39301 cgggccctct ttgcccgcct tatccacccg cgccagcggt tcataactcc cctggtccct
    39361 gtagatgtac aggctgcctg ttccctccgg cctgctctcc tgcgccagcc gcattccgtc
    39421 ccataaaaat tttgtccggt tgtacggctt accgtcaggg ctgatgtgct gtttttccgt
    39481 ccgcctcccc agtgcatcgt acacataccg gtatgtcccc cgcgctgtcg tcacttccac
    39541 catccggtgt tccgcatcat aacggtaact ctgcgttccg ctccggccct ccttttccac
    39601 cgcacggcca aattcgtcat aacggtagtg cacaccacgg taagacatca gccggttgaa
    39661 cggcaccacg ctgttttctg ccgtggccct ttcccccgcg cgcctcccca gcaggtttcc
    39721 cgccgcgtcc acctccgttt ccggctggtg ccacaccggg tgccactgcg cctgctccac
    39781 ccgccccagc gggtcgtgcg tctccgtgat atgcccctgc aggtcataca catagcgcca
    39841 tttgccgccc tgagggtcgg tcatccccag caacagtcgt tcttcgtcgc tccagcggaa
    39901 tgtcgtcacc tgtccggctt cgtcctggta cgccgtcacc tgattctgcg catcccactg
    39961 gtgataacgt gtgcgcccca gtccgtccgt cacgcacagc ctgcgctgcg ccaggtccca
    40021 cgccagccgg tactgttcac cgctgtccgt gtggtgcccc gtcacccgcc atgccgcgtc
    40081 atccggacct tcataccagc cccactggta ataataccct tcgccgcccg gctggcggtg
    40141 cagcgtcatg cagtggtttt cgtcgtaagc aaactcacgc agcaggtgcc ccgtgctgtc
    40201 gtacacgccg ttcaggtggc cggcctcgtc atagctgtaa cgacgcagca gtaaaggtgc
    40261 agtctcaggg aaatgctgat aaatctgtgt cacccgccgg ggatgcgccg ccagttcgta
    40321 atacagacgg atgcacggac gctgctgttc gccgctgatt tccacaatgc ggccccggtc
    40381 gtcgtagtac agattcagac agttgctgtt acggtcgccc agcattttca gccgctggcg
    40441 ctgcgggtgg tgcgggtcag cctcaaacag aaaacactgc ccgtcatcgc tgctgataag
    40501 ccagtgcccc tgctcaccac gccttaccgc cagcccttca ccggggctgt aaaacccgcc
    40561 ccccggcagt acggcttcca tatccaagcg tcgccccatg cccgacacaa aggtcatgca
    40621 gttttcgtct gcgctacccg gtgtgcacac gaggctgact tcatacatta cactccagcc
    40681 ctgaccaaac attccttccg tacgttcgtc acgactgctg tacacgcgct gccagtccag
    40741 cggaaagtgg cccggcagac tgaaatcgaa gtcatcgtca tcggccaggt atttggcccc
    40801 ggtggcggca ttgaccggat gcggaagggc actggtggcc ttctgcgtca gcatgctcat
    40861 cccgacactg gcagcaaaac aggcgatttt agaaagcatt ttgcccgggc gaagaaacat
    40921 cagcgccacg gttgccacca gggttatctg cgacctgccg cttctgatat ccctgaccac
    40981 cagtaacgga ccgccaatgc gcacatccgg tgacacaaac gccccgttcc ccggctcatt
    41041 caccaccctg gcctcgcagg tacagcgtgc cccgctgcgt gtcgccggct ggctgttgat
    41101 acgcacatcc cgcgcgcctt ccgcgatata ttccgctgcg ggcggctcgt catcgccttt
    41161 gaacagatta ctgatgctgc caataaccgc attcactccc tgaaaggcgg tttccagcat
    41221 accgcccgga ttcagcaggt taagggccgt tcccaggggg tcatccagaa aggcggcttt
    41281 tttttgtgcg agaaactgcg tcagtgagga aggatgttta tggcagtcga ttttatcgtc
    41341 aggggtcggg gtggatcccg gggccgcact ggccacagtg ggctgccata gttccgacac
    41401 aaaatgtgtc acgccatcca ccatttcaga acgcccttct tccgtactga gctggtcgtg
    41461 cagagcccgg gacatcagca caggccctgt caccgggtta accgccaggg ctatggcccc
    41521 cgccatttcc agatattccc cggccacatc ccggaacgtt ctggcatcct cctgacgctg
    41581 cgcttcttcc tgttctgcct tcgcctgcgc ttcctgcgcc tctatgtcct gccgcgatgc
    41641 cgcagtcgcc gctgcccgcg cggcaggttt gctgttgatc catgtgtcac cagagccggt
    41701 gaggatgtaa ccttcaatct gaggcgggaa aagcatattt gcgagggctg aacaggcttc
    41761 gctgaccttt tctgtcacgc cggtgtacac catcgccaca cccgccacaa aaccggtcag
    41821 cacgacggcc gtgcacccca tcgtgatccc cactgacgtc gccagtaacg aggttgtgcc
    41881 gacaatgagg gcatcaataa cgacatttgc ggccacctcc agcaccgccc ccagcacatc
    41941 tgccataaac gacgtatgca gcagggcatc accctgtctt gccgcccaga atgcctcttc
    42001 catgtcccgt tccctgtatg attaattcgt cctgacttcc tgccgcatcc tgaagcgcag
    42061 attcttttta atttcagccc agcgcgtcat atctgccggg gtcagcggtg tggttctggc
    42121 gtaggtgaag gccatcatga cccgctcacc cggcaataca aatgccacct gacactggta
    42181 cacctgctcg ttaccccgga gaaactggct ctgtatttcc aaaccgcctc gttgagataa
    42241 tattccgtca taatgtaaat agtccgtttt aataaaggaa ttatttattg acaggttctg
    42301 tttttttaat catgaatgta atacccatca ccgctttaca gcacacttta tctttccagt
    42361 cataatcact aataatatta agcaattagc aatatttaca acgaccagag cggatacata
    42421 gttatgtatt taacaatccc aatatattca ctatgcaaat aaaaccacta gtaacagaca
    42481 cgctaccgcc tccatataaa aatggacacc ttataaaatt gtatcttgca tagaaaaagc
    42541 tgaaatatcg gatgcgtttt aattatcaac tataaatcta taagcttttt attaacaac
//
LOCUS       DAJTSI010000019        37627 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000019 DAJTSI010000000
VERSION     DAJTSI010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37627
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.019"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..175)
                     /locus_tag="OO327_000909"
     CDS             complement(<1..175)
                     /locus_tag="OO327_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCR6017544.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(259..1143)
                     /gene="yhdJ"
                     /locus_tag="OO327_000910"
     CDS             complement(259..1143)
                     /gene="yhdJ"
                     /locus_tag="OO327_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6017545.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1229..1525)
                     /gene="fis"
                     /locus_tag="OO327_000911"
     CDS             complement(1229..1525)
                     /gene="fis"
                     /locus_tag="OO327_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR6017546.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1551..2516)
                     /gene="dusB"
                     /locus_tag="OO327_000912"
     CDS             complement(1551..2516)
                     /gene="dusB"
                     /locus_tag="OO327_000912"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR6017547.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2845..3726)
                     /gene="prmA"
                     /locus_tag="OO327_000913"
     CDS             complement(2845..3726)
                     /gene="prmA"
                     /locus_tag="OO327_000913"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR6017548.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3738..5189)
                     /gene="panF"
                     /locus_tag="OO327_000914"
     CDS             complement(3738..5189)
                     /gene="panF"
                     /locus_tag="OO327_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR6017549.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5179..5421)
                     /locus_tag="OO327_000915"
     CDS             complement(5179..5421)
                     /locus_tag="OO327_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR6017550.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5530..6879)
                     /gene="accC"
                     /locus_tag="OO327_000916"
     CDS             complement(5530..6879)
                     /gene="accC"
                     /locus_tag="OO327_000916"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR6017551.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6890..7360)
                     /gene="accB"
                     /locus_tag="OO327_000917"
                     /gene_synonym="fabE"
     CDS             complement(6890..7360)
                     /gene="accB"
                     /locus_tag="OO327_000917"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR6017552.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8338..9312)
                     /locus_tag="OO327_000918"
     CDS             complement(8338..9312)
                     /locus_tag="OO327_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6017553.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9464..11404
                     /gene="csrD"
                     /locus_tag="OO327_000919"
     CDS             9464..11404
                     /gene="csrD"
                     /locus_tag="OO327_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR6017554.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11709..12752
                     /gene="mreB"
                     /locus_tag="OO327_000920"
     CDS             11709..12752
                     /gene="mreB"
                     /locus_tag="OO327_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR6017555.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12818..13921
                     /gene="mreC"
                     /locus_tag="OO327_000921"
     CDS             12818..13921
                     /gene="mreC"
                     /locus_tag="OO327_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR6017556.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13921..14409
                     /gene="mreD"
                     /locus_tag="OO327_000922"
     CDS             13921..14409
                     /gene="mreD"
                     /locus_tag="OO327_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR6017557.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14418..15011
                     /gene="yhdE"
                     /locus_tag="OO327_000923"
     CDS             14418..15011
                     /gene="yhdE"
                     /locus_tag="OO327_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR6017558.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15001..16470
                     /gene="rng"
                     /locus_tag="OO327_000924"
     CDS             15001..16470
                     /gene="rng"
                     /locus_tag="OO327_000924"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR6017559.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16538..20338
                     /gene="yhdP"
                     /locus_tag="OO327_000925"
     CDS             16538..20338
                     /gene="yhdP"
                     /locus_tag="OO327_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR6017560.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20494..21939
                     /gene="tldD"
                     /locus_tag="OO327_000926"
     CDS             20494..21939
                     /gene="tldD"
                     /locus_tag="OO327_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR6017561.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22067..22996)
                     /gene="aaeR"
                     /locus_tag="OO327_000927"
     CDS             complement(22067..22996)
                     /gene="aaeR"
                     /locus_tag="OO327_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCR6017562.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23179..23382
                     /locus_tag="OO327_000928"
     CDS             23179..23382
                     /locus_tag="OO327_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR6017563.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23390..24322
                     /gene="aaeA"
                     /locus_tag="OO327_000929"
     CDS             23390..24322
                     /gene="aaeA"
                     /locus_tag="OO327_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCR6017564.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24328..26295
                     /gene="aaeB"
                     /locus_tag="OO327_000930"
     CDS             24328..26295
                     /gene="aaeB"
                     /locus_tag="OO327_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR6017565.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26387..26659
                     /locus_tag="OO327_000931"
     CDS             26387..26659
                     /locus_tag="OO327_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR6017566.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="OO327_000932"
     CDS             complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="OO327_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR6017567.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27343..27813)
                     /gene="argR"
                     /locus_tag="OO327_000933"
     CDS             complement(27343..27813)
                     /gene="argR"
                     /locus_tag="OO327_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR6017568.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28248..29186
                     /gene="mdh"
                     /locus_tag="OO327_000934"
     CDS             28248..29186
                     /gene="mdh"
                     /locus_tag="OO327_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6017569.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(29248..30315)
                     /gene="degS"
                     /locus_tag="OO327_000935"
     CDS             complement(29248..30315)
                     /gene="degS"
                     /locus_tag="OO327_000935"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR6017570.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30405..31752)
                     /gene="degQ"
                     /locus_tag="OO327_000936"
                     /pseudo
     CDS             complement(30405..31752)
                     /gene="degQ"
                     /locus_tag="OO327_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31906..32304)
                     /locus_tag="OO327_000937"
     CDS             complement(31906..32304)
                     /locus_tag="OO327_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR6017571.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32498..33625
                     /gene="zapE"
                     /locus_tag="OO327_000938"
     CDS             32498..33625
                     /gene="zapE"
                     /locus_tag="OO327_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR6017572.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33844..34272
                     /gene="rplM"
                     /locus_tag="OO327_000939"
     CDS             33844..34272
                     /gene="rplM"
                     /locus_tag="OO327_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR6017573.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34288..34680
                     /gene="rpsI"
                     /locus_tag="OO327_000940"
     CDS             34288..34680
                     /gene="rpsI"
                     /locus_tag="OO327_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR6017574.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35075..35713
                     /gene="sspA"
                     /locus_tag="OO327_000941"
     CDS             35075..35713
                     /gene="sspA"
                     /locus_tag="OO327_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR6017575.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35719..36216
                     /gene="sspB"
                     /locus_tag="OO327_000942"
     CDS             35719..36216
                     /gene="sspB"
                     /locus_tag="OO327_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR6017576.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36259..37626)
                     /gene="dcuC"
                     /locus_tag="OO327_000943"
     CDS             complement(36259..37626)
                     /gene="dcuC"
                     /locus_tag="OO327_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6017577.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
BASE COUNT         9114 a         9413 c         9901 g         9199 t
ORIGIN      
        1 ttctgtcctg aaaattcagt tcgtcacagt taaccagttt tagtggatta actgagttca
       61 tattacggca tttgtctgtt tctgttgtca gcagttcaat ccactgcatc agtaaagtat
      121 caaacagaaa aacgaagaca gcgaaaacga cgagccacca atacttgcga atcattacct
      181 gagcctgtgc atgtttaacc aagtctggca ggaaatatac acgaaatgaa taatctgaaa
      241 actaggctta cacttccttt actttgcaat gagatcgggg tcaacttcgc ttaaccggct
      301 tcgttttgac aggttgccgg tctttctttt tttcactttc gccagttctt ccgcggagta
      361 atgcgacgcg acatccagcc gtcgaagccc cattttgatg tactcgctgt tgatctcaat
      421 accaatgaat tttcgtccgc tggcgatggc tacggcaccg gtagtaaagc taccggcaaa
      481 cgggtcgaga acgatatcgc ctgggttgga agaggcgaga ataatgcgtt tcagtaaggc
      541 tttcggtttt tgcgtcgggt ggttctcata ttcatccatc aaataacgca cgcgcggaaa
      601 atcccagacg ttacccggta ctttttgatg attgtatggc tgtggaggat ttttgcgata
      661 atcgatcaac gcgcgctgcg atccggtttt ggcttctacc agaatagcat caccgttgaa
      721 tgtgtagttc tttgcgtctt tcaccatcat caggatgggt tcgtacatgg agccgtagtg
      781 ttttttcgcc tgtactccag aactgtcata tgaccagacg atgcgacttt tgatggtaaa
      841 aagcttgcgg cactggagat cgataaaggg catgttttcc gtactgttca taatgtacat
      901 gctgccctgc tttttcagaa cgcggtggca ctctgcaatc acttcaaaca gccagtcgat
      961 aaacagatct tctttccagg cttcgatcag accatcaaaa tttttaccga tgttatacgg
     1021 tgggtcggca aagagcagat cgacgctttc agtaggtagc tttttaagtt cggcaaaggc
     1081 atcaccgtga ataatggtct tagcttcatt accaaaccgg gtcggttcac atcctgttct
     1141 catggtcact ccctttgtga cacctataaa aaaggcgctt ccccatgccg agtagcgcct
     1201 ttttaatcaa gcatttagct aacctgaatt agttcatgcc gtattttttc aattttttac
     1261 gcagcgtacc acggttgatg cccatcatca gcgcagcacg ggtctggtta ccacgggtgt
     1321 attgcatcac catgtccaac aggggctgtt ctacttcagc cagtaccagc tcatagaggt
     1381 cattcacatc ctgaccattc agttgagcaa aatagttctt cagtgcctgt ttaaccgagt
     1441 cacgcagggg tttttgggtt acctgatcct gagagttaac ggtagaaacg gtcagtacgt
     1501 cagaatttac gcgttgttcg aacatagttc tgtcagctct ttatttctgt ttacgcaaaa
     1561 ttttcgaagt atgcctccaa cgcctccagc tgttcgctgg catcctcaat ggcgttgaat
     1621 gtgcgccgaa actggtcatt tggagcgtgt tcctggagat accaggaaac gtgtttacgt
     1681 gcaattcggt acccttttgc cggaccataa aagtcatgca gttcccgaac gtgcgcgcaa
     1741 agcaagcgct taacctctgc caaaggcagt gggggcagca actccccagt gtccagataa
     1801 tgctggattt cccgaaagat ccagggtctt ccctgagctg cgcggcctat catcagggca
     1861 tccgcccctg tatagtcgag cacagctctg gctttaagcg ggtcagtaat gtcgccattc
     1921 gcgataaccg gaatggaaac tttctgctta actgcccgaa tactgtcgta ctcagcttct
     1981 ccattgaaca aacaggcgcg tgtacggcca tgaatggtca gagcctgaat gccacagtct
     2041 tcagccagtt gggcaatctc ttcgcagtta cggtgttccg gtgcccagcc ggtgcgaatc
     2101 ttcagggtaa caggaacgtc cactgcattg acgacctcgg taaggatcga tttaacgaca
     2161 tccgggtact gcaagagggc tgaacctgcg agcttgcgat tcactttttt agccgggcaa
     2221 cccatattga tatcaataat ctgggcaccg ctttccacgt taatacgtgc tgcatctgcc
     2281 atttctttcg gagcgctacc agcaatttgc acggtgcgaa taccgggttc atcaatgtgc
     2341 accatccgta aacgagattt gtcgctttcc caaacctgtg ggttagaaga catcatctcg
     2401 gatactgtca atccggctcc catctcgtag cacaacgtcc gaaaaggtct gtctgtaatg
     2461 ccagccatgg gcgctgcgat caggcgattt ctgagctgat attgtccgat gcgcatgagt
     2521 taagaaatga ccatactgtg actgcaaggc ggcgtatatt acgcattttt tgcacgagat
     2581 gaaaggccaa actttgacca atcctctgag atggatcaaa gaattgcatt taaaatgagc
     2641 gtggtgcgat aattactcat aaaaatcatc atattagaaa atagtgacta aaatttacac
     2701 tcaaagaaat ttgagtaagt tctcaatttt tctttatgaa tgaaaatttt ggcacgcaaa
     2761 tttgcgtaaa taatcggcaa atttacgtgc cttttgtgag cttgctcgca cttcgccccg
     2821 cgtcacccta cggcgatgcg aaggtcaatt cttacgaccg gtaatacggc accactcttc
     2881 tttttccacg accgggtcca gtgcgaagct atcggcataa gcttcacaaa cgctctctgc
     2941 ctggcttgcc agaataccgg aaaggcccag caaaccgcct gaaactggca ggacgctgat
     3001 taacggtgcc agttcacgta atgggcctgc aaggatgtta gcgaccacca cgtcggcttt
     3061 catttcttct ggctgatctt tcggtaagta gagttccaga cggtcagaaa cgccattacg
     3121 ttcggcgtta tcgcggctgg cctgaatcgc ctgcggatcg atatcaatac caatggcttt
     3181 tgctgcaccc agtttcagcg ccgcgatcgc cagaatgccg gaaccacagc caaagtcgat
     3241 gactgtttta ccggttaaat cgaggctgtc gagccattgc aggcacagag aggtggttgg
     3301 atgggtaccc gtaccaaacg ccagccctgg atctaacatc acgttgacgg cgttttcgtc
     3361 cggcacatca cgccagctag ggcagatcca cagtcgttca ccaaagcgca tcgggtggaa
     3421 attatccatc cattcgcgct cccagtcttt atcttctagt tgttcgattt tatgcgcgaa
     3481 gcctgcgccg agcagcggat ggttttccag aatcgccacc acgtcgttca tatcggtttc
     3541 agcgtcgaac agaccaatca catcggtgtc gccccacagg cgcgtttcgc ccggcagcgg
     3601 ctcaaatact ggcgtatcgt gggtatcctg aaaagtgata gaaacggcac ccgcttccat
     3661 cagcgcatcg ctaagatctt ccgcgttcgc gccggtggtg ttcagtttca gttggatcca
     3721 aggcatggca aaactcttta tttatcagta gtcaaaacgg tagcttgcgg gacggatgta
     3781 ccgaaacggt ttccgaccag gaaagccagc aaacttagta gtaacgaggg cacgataggg
     3841 tggaagccca ggtactgaat attcagcgtc gcgagtacgg catacagcac gccgccaacg
     3901 atcatcgcac ttagcgcgcc tttggcgttg gcgcgttccc agtaaagacc cagcaccagc
     3961 ggccacagga aaacggcttc cagcccaccg aaggccagca aattcagcca gatgatcatt
     4021 tctggcggct tccaggcggc aagcagcagc aacgcgccga gaactaacgt aattaccgcc
     4081 gacatccgct tcagacgcgt ctcgttttgc atttgatccg gacggatatt cagatagaga
     4141 tctttaatga tcgtagcgga actttgcagc agttgggcgt taattgtcga catgatcgca
     4201 gccataggtg cagccagaaa gatcccggca gcaaaaggtg gcagcacttt taccattaac
     4261 gtcgggatca ccagatctgg tacggtgaga tcggggatca ctgcacgacc taacgctccg
     4321 gccaggtgca taccgaacat caggattgcg actataatcg taccgatgat gatcccccgg
     4381 tgtactgctt tgctgtcttt ataagagata cagcgcaccg cagtatgcgg caggccaatc
     4441 acgccaaagc acaccagtac ccagaacgac gtcataaagg caggcgacag aatatcgtca
     4501 gcgccttgtg gcgtaaccag ttgcggatcg atggtttgca aggtctgtac tgcgttgctt
     4561 aagccgccag cggcatgtac cacgccaata agcagcacaa cggtgccaat cagcatcaca
     4621 agcccttgca tggtgtcgtt cagcacgctg gcgcgaaagc caccaaaggc ggtatataac
     4681 gcaatgctga taccaaaaat cagcagcccg gtttcataag gaatacccgc cgcggtttcc
     4741 agcaggcgcg caccgccgat aaactgcacg gtcatcgcac caacgaacgc aaccagcaaa
     4801 ctcaaactcg ccagccacac cagaagacga ctctggtagc gggcaaacag catatcgttc
     4861 agcgtcactg cattgtagcg gcgcgcaaga atcgcaaact tcttgccgag aataccgagt
     4921 gaaagccaga cagcaggaag ctgaatcatc gccagcaata cccagcccag cccgtattta
     4981 taagcagctc ctggcccgcc gataaacgaa ctggcactga tataggtcgc ggtgagagtc
     5041 atcgccagca caataccgcc catagagcgg ctgccgagga aatactcatt aaggaaggtg
     5101 ccggtgctcc gtttacgcat cgcataaacc gagataccga acaccaccac cagataggcg
     5161 accagcggta gaattacttc aagctgcatc gtcatcctcc agtgggatat cgcgatagat
     5221 aaatttcacc atcgcccagc acagtccaat aaacagtagc ggcgtcagga tgcaggccat
     5281 ctcaaaccag cgcggaaagc cggtaaaacc gggggcaacg ccaggtaagt aagcgtctac
     5341 taaccaaact gccagataca aaagggtcag ccccagcgcc cagcgggcct ctttatgggc
     5401 ctgaacaaaa cgagtgtcca ttttttgtcc ctgatgggtg aagaaagcga ggattgtacc
     5461 ttatgggggt tgtcgatccc cagtaataaa aaaggccgga aaatccggcc ttttgacgct
     5521 tttgcagtct tatttttcct gaagaccgag ttttttctcc agatagtgga tgttagtgcc
     5581 accatgctgg aagttctcgt cattcatgat gcggatctgc agatcaacgt tggttttgat
     5641 accgtcgatg atcagctcct gcagcgcatt cttcatgcgg gcaatcgcca cgtcacggtt
     5701 ttcaccgtag caaatcagct taccgatcat tgagtcatag tacggcggta cggtgtagcc
     5761 cgcgtagata tgagactccc aacgtacgcc aaaaccgcca ggtgcgtgga aacgggtgat
     5821 tttgcctggg cttggcagga aggtgttcgg atcttcggcg ttgatacgac attccaccgc
     5881 atggccgcga acgtgaactt cttcttgctt gatcgacagc ggttgaccgg cagcgatacg
     5941 cagctgttct ttgatcaggt caacgccggt gatcatttct gtaaccgggt gttctacctg
     6001 aatacgggtg ttcatttcga tgaaatagaa ctcgccgttt tcgaacagga actcgaaagt
     6061 acctgcaccg cgatagccga tatcaacaca cgctttagcg caacgttcgc cgatgtagcg
     6121 acgcagttcc ggggtaatgc ccggtgctgg tgcttcttcg accactttct ggtggcggcg
     6181 ctgcatggag cagtcacgtt ccgccagata gatagcgttg ccctgaccgt cagccagtac
     6241 ctgaatctcg acgtggcgag gattctccag gtatttctcc atgtaaacca tatcgttgct
     6301 gaaagcagct ttcgcttccg cacgggtcat ggagatggat tgtgccagtt cagcgtcgcc
     6361 gcgcactacg cgcataccgc gaccgccgcc gccgccggag gctttgataa tcaccggata
     6421 accaatgcgt ttagcaatgg cgcggttttt atccatatcg tcgcccagcg ggccgtcaga
     6481 accaggtacg caaggaacgc ccgctttttt catcgccgcg attgcggata ctttgtcgcc
     6541 catcaggcga atggtttctg ctttcgggcc gatgaagata aagccggagc gttcaacctg
     6601 ctcggcaaag ttggcgttct cggagaggaa gccgtaaccc ggatggattg ctactgcgcc
     6661 ggtgatttca gcggcgctga tgattgccgg gatgttcaga taacttttta ctgacggagc
     6721 agggccaata cagaccgttt catctgccag taatacgtgt tttagatcgc gatccgtgct
     6781 ggagtgcaca gcgacagtct tgatgcccag ttctttacag gcacgaagaa tacgcaatgc
     6841 aatctcgccg cggttggcaa taacaatttt atccagcatg ttcgcctcgt tactcgatga
     6901 cgaccagcgg ctcgtcaaat tctaccggtt gtccactttc gaccagaatt gctttcacgg
     6961 taccggattt gtccgcttcg atctggttca tcattttcat ggcttcaacg atgcacaggg
     7021 tatcgcccac gttgactttc tgacccactt cgatgaacgc ttttgcgtcc gggcttgggg
     7081 tgcggtagaa agtaccaacc atcggggaac gtacgatgtg accactgatt tccgctgctg
     7141 ctggcgcttc catggaagga acggtcgccg gagcggctgc gttagattga gctggctgct
     7201 gcatcattgg tgcagcgtaa gcttgttgca tcacagggaa acttgcggca ggagctgcac
     7261 ggctaatgcg tactgactct tcgccttcag aaatttccag ttcggagatg cctgattctt
     7321 caaccagctc gatcagtttt ttaatcttac gaatatccat gagtgggttc cgtactcttt
     7381 gtttagtgtg attgtgacag gcgtttcacc gccgtctgta aagcgtatgc cccgacagct
     7441 gtatgcatag cgataaattc cagcaggccg ggacgccggt ctattttgcc ccagagtgtc
     7501 aatgttagac ttgacggaca ttgtgcagcg atctgctgct atcctccggc aaaaaacaaa
     7561 atataccttc gttgcgccaa tgtcaccttt tccgcagcgc aaaaactgcg tcagggagga
     7621 cgaggtcgca cattataacg atttcgtagc aattggcagc taaatactgg tcttatcagg
     7681 gaatataatc aacagctaac atgtaaataa ccttcaacac cgtgtaattt gcaacaagcc
     7741 gcacaattca cgaaattagc gccatcatcg acggaacttc ttataacgta aggtgaaaag
     7801 ctgaaaagcc agccctgcga agatgagggg ctgcaatgag ataatcttca cagaccacaa
     7861 ataatgtagg gatgccagga tcgcgacaag atagacgaca ttacgcagcg gttgctgggg
     7921 attacccaat tttcactgca tcgtctggat agatgtgaat gctaaagcac gcaaaatcaa
     7981 ccaactgaca attctcagca ttaaataggt tcgagtggtt aatttgtagt caaataaaat
     8041 cagattattc accccatggt accagaaatc agtaacaggt taagcgtagt gtcgcccagg
     8101 caaagcacca taaacctaac aggcgacgga ggcgtatcaa taatggctgt ttagaggagt
     8161 atcaatgcgc atcaatttgt ggtcagtcga aatcatgact ttccagtttg gatgatgttg
     8221 tacaacttcc tgaccaaggt gttcgacgaa agctgtccgg ctaaccgctt ttcccgttaa
     8281 ccgattgaat aatgatgtca aaatgagttc cctctcttat tattccttgc taaatggtta
     8341 gttaaccttc accagcgtgc gaccctggat ctggttatta atgatggcct cggcaaagtt
     8401 cggtgcctct gacagagata tctcttttgc cgcctgggta tagaatgatt ccggtaaatc
     8461 ggcgaccagt cgctgccagg cttgtgcgcg gcgttctggt ggcgtcatta ctgaatccac
     8521 cccttgcaaa cggacattac gcagaataaa tggcatgacc gtggttggca gagtaaaacc
     8581 acccgccaga ccacaggccg ccacgcagcc gccgtaattc atttgcgcca gcacttttgc
     8641 cagcactttg tcgccaacag tgtcaattgc cccagcccag acttgttttt ccagaggacg
     8701 ggattcggca aactcatcac gagggagaat accgctggca cctaaacttt tcagatattc
     8761 atgggtactt tcgcgaccgg aaacggcaac gacctgataa cccaacttat gcagcagcgc
     8821 cacggcggta ctgccgacgc caccactggc acccgtcacg acaatctcac cgtcctgcgg
     8881 gcgaacaccg gcatcttcca gcgccatcac acacagcatg gcggtaaaac cggcagtacc
     8941 gataatcatt gctttacgcg cgtccagccc ttgcggcatg gcaaccagcc agtcaccttt
     9001 cactcgcgcc tgctccgcca gcccacccca gtggttttca ccaacgcccc agccagtgag
     9061 taacacctcc tgaccggcat gaaaacgcgg atcttcgctg gtgcgtacag ttccggcaaa
     9121 atcgatccca ggaatcatcg gaaaattacg gatgattttt cccttaccgg taatcgccag
     9181 cgcatcttta tagttcaggc tcgaccagtg aacatcgacc gtgacatcgc cctccggcag
     9241 gcgacgttcg tccagagtct gtactgatgc gagagttttg ccgtcctgct gttctaaaag
     9301 taacgcctgc ataagtggtc ctcatgtgca tgatggattg gaaaataatt tctgaagact
     9361 atactcgcta atggaaaagc aaagccgatg aagcgcaata aattgcgaga taaatctgat
     9421 ttgctagtat gcccgcttcc tcactatcgg agttaacaca aggatgagat taacgacgaa
     9481 attttcggcc tttgttacgc tgctcaccgg gttaacaatt tttgtgactt tgctgggctg
     9541 ttcgctaagt ttctacaacg ccattcagta taagtttagt catcgcgttc aggcggtggc
     9601 gacggcgatt gatacccacc ttgtgtcgaa tgacttcagc gcattaaggc cacaaattac
     9661 cgaattaatg gtgtcagcag atatcgttcg tgtagacctg ctgcatggtg ataaacaggt
     9721 ttataccctg gccagaaatg gtagttatcg tccggttggc accagcgatc tgtttcgtga
     9781 actgagcgtt ccgttgataa agcatccggg gatgtcgttg cgtctggttt atcaggatcc
     9841 gatgggcaac tatttccatt cgttgatgac caccgcgccg ctcacggggg cgattggctt
     9901 tatcattctt atgctctttc tggcggtacg ctggttacaa cggcaacttg ccgggcaaga
     9961 attgctggaa acccgggcta ctcgtatctt aaacggtgag cgtggctcta atgtgttggg
    10021 aaccatctat gaatggccgc ccagaaccag cagtgcgctg gatacgctgc ttcgtgaaat
    10081 tcagaacgca cgcgaacaac acagccgtct tgatacgctg atccgctctt atgccgcaca
    10141 ggacgtgaaa accggcctca ataaccgact ctttttcgat aatcagttag caacgttact
    10201 ggaagatcag gagaaagtag gtacccacgg gatcgtgatg atgattcgtc tgccggattt
    10261 caatatgttg agcgatacct gggggcacag ccaggttgaa gaacagttct tcactctgac
    10321 gaatctgctg tcgacattta tgatgcgcta ccctggcgca ctgctggcgc gttaccaccg
    10381 cagtgatttt gctgcgttgt taccgcaccg gacgttaaaa gaggcagaga gcatagccag
    10441 tcagttaatc aaagccgtcg ataccttgcc gaacaataaa atgctcgatc gcgacgatat
    10501 gatccacatt ggtatctgtg cctggcgtag tggtcaggat accgagcagg taatggaaca
    10561 tgcagagtct gccacgcgta atgcgggatt gcagggtggc aatagctggg ctatttatga
    10621 tgactcgttg cctgaaaaag gacgcggtaa tgttcgctgg cgtacgctta tcgagcaaat
    10681 gctcagtcgc ggcggcccgc gcctttatca aaaaccggcg gttactcgcg aaggtcaggt
    10741 tcatcatcgc gaactcatgt gccgcatctt cgatggtaat gaagaggtta gctcggcgga
    10801 gtatatgccg atggtcttgc agtttggctt atcggaagag tatgaccgtc tgcaaatcag
    10861 ccgtcttatt ccactattgc gttactggcc agaggaaaat ctggcgattc aggttaccgt
    10921 tgagtcgctg attcgtccgc gttttcagcg ttggctgcgc gatacgttaa tgcaatgtga
    10981 aaaatcacaa cgaaaacgca taattattga acttgcagag gccgatgtag gtcaacatat
    11041 cagtcgttta caacctgtta ttcgtttagt gaatgcttta ggggtacggg tagccgtcaa
    11101 ccaggctggt ttgacgctgg taagcaccag ttggatcaaa gaacttaatg ttgagttact
    11161 caagctccat ccggggctgg tcaggaacat tgagaagcga acggagaacc agctgctggt
    11221 tcaaagcctg gtggaagcct gctccgggac cagcacccac gtttacgcca ccggcgtgcg
    11281 ttcgcgaagc gagtggcaga ccctgattca gcgcggtgtt acaggcgggc aaggggattt
    11341 tttcgcgtcc tcacagccac ttgatactaa cgtgaaaaaa tattcacaaa gatactcggt
    11401 ttaacctgcc gtttaatccg ttttcacgta gaataacgcg cgctgcgtct catgggagtg
    11461 tgcttgtctg ctcgccagat tgttgcagca catatgcaga tgaatgacct tacgcggttg
    11521 caaacaggcg aggaatgctg ctgatgcatt aagcctttct ggactcaggc agagatttgt
    11581 aacaaaggaa acgaactgca ctaattttca ccgtagcaga tgatttttgc gccttgtcgc
    11641 tgctgcgtgt ggttggtaaa gtaagcggat tttcttttcc gccccagctt tcaggattat
    11701 cccttagtat gttgaaaaaa tttcgtggca tgttttccaa tgacttgtcc attgacctgg
    11761 gtactgcgaa taccctcatt tatgtaaaag gacaaggcat cgtattgaat gagccttccg
    11821 tggtggccat tcgtcaggat cgtgccggtt caccgaaaag cgtagctgca gtaggtcatg
    11881 acgcgaagca gatgctgggc cgtacgccgg gcaatattgc tgccattcgc ccaatgaaag
    11941 acggcgttat cgccgacttc ttcgtgactg aaaaaatgct ccagcacttc atcaaacaag
    12001 tgcacagcaa cagctttatg cgtccaagcc cgcgcgttct ggtttgtgta ccggttggcg
    12061 cgacccaggt tgaacgccgc gcaattcgtg aatccgcgca gggcgctggt gcccgtgaag
    12121 tcttcctgat tgaagaaccg atggctgccg caattggtgc tggcctgccg gtttctgaag
    12181 cgaccggttc tatggtggtt gatatcggtg gtggtaccac tgaagttgct gttatctcct
    12241 tgaacggcgt ggtttactcc tcttccgtgc gcattggtgg tgatcgtttc gacgaagcta
    12301 tcatcaacta tgtgcgtcgt aattacggtt ctctgatcgg tgaagccacc gcagaacgta
    12361 tcaagcacga aatcggttcg gcttacccgg gcgatgaagt ccgtgaaatc gaagttcgtg
    12421 gccgtaacct ggcagaaggt gttccacgcg gttttaccct gaactccaac gaaatcctcg
    12481 aagcactgca ggaaccgctg accggtattg tgagcgcggt aatggttgca ctggaacagt
    12541 gcccgccgga actggcttcc gacatctccg agcgcggcat ggtgctcacc ggtggtggcg
    12601 cactgctgcg taaccttgac cgtttgttaa tggaagaaac cggcattcca gtcgttgttg
    12661 ctgaagaccc gctgacctgt gtggcgcgcg gtggcggcaa agcgctggaa atgatcgaca
    12721 tgcacggcgg cgacctgttc agcgaagagt aatcggatgc aggcagggga agcgtctgtt
    12781 taccctgcct ggtctgatac gagaatacgc ataacttatg aagccaattt ttagccgtgg
    12841 cccgtcgcta cagattcgcc ttattctggc ggtgctggtg gcgctcggca ttattattgc
    12901 cgacagccgc ctggggacgt tcagtcaaat ccgtacttat atggataccg ccgtcagtcc
    12961 tttctacttt gtttccaatg ctcctcgtga attgctggat ggcatatcgc agacgctggc
    13021 ctcgcgtgac caactagaac ttgaaaaccg ggcgttacgt caggaactgt tgctgaaaaa
    13081 cagtgaactg ctgatgcttg gacaatacaa acaggagaac gcgcgtctgc gcgagctgct
    13141 gggttccccg ctgcgtcagg atgagcagaa aatggtgact caggttatct ccacggttaa
    13201 cgatccttat agcgatcaag ttgttatcga taaaggtagc gttaatggcg tttatgaagg
    13261 ccagccggtc atcagcgaca aaggtgttgt tggtcaggtg gtggccgtcg ctaaactgac
    13321 cagtcgcgtg ctgctgattt gtgatgcgac ccacgcgctg ccaatccagg tgctgcgcaa
    13381 cgatatccgc gtaattgcag ccggtaacgg ttgtacggat gatttgcagc ttgagcatct
    13441 gccagcgaat acggatattc gtgttggtga tgtgctggtg acctccggtc tgggcggtcg
    13501 tttcccggaa ggctatccgg tcgcggttgt ctcttccgta aaactcgata cccagcgcgc
    13561 ttatactgtg atccaggcgc gtccgactgc aggactgcaa cgtttgcgtt atctgctgct
    13621 gctgtggggc gcggatcgta acggcgctaa cccgatgacg ccggaagagg tgcatcgtgt
    13681 tgctaatgaa cgtctgatgc agatgatgcc gcaggtattg ccttcgccag acgcgatggg
    13741 gccaaagtta cctgaaccgg caacggggat cgctcagccg actccgcagc aaccggcgac
    13801 aggaaatgca gctactgcgc ctgttgcgcc gacacagccc gctgctaatc gctctccaca
    13861 aagggctacg ccgccgcaaa gtggtgctca accgcctgcg cgtgcgccgg gagggcaata
    13921 gtggcgagct atcgtagcca gggacgctgg gtaatctggc tctctttcct cattgcgctg
    13981 ttgctgcaaa tcatgccctg gccggataac ctgattgttt tccggccaaa ctgggtgtta
    14041 cttatcttgt tgtattggat cctggccttg ccccatcgcg taaatgtggg cacaggtttt
    14101 gtgatgggtg ccatactgga tctgatcagc ggctcgatgc ttggcgtacg cgtattggcg
    14161 atgagcatca ttgcttacct ggtggcgctg aaataccagc ttttccgcaa cctcgcatta
    14221 tggcagcagg cgctggtcgt catgttgctt tcgctggtgg tggatattat tgttttctgg
    14281 gcagagtttt tagtgattaa cgtctctttc agaccggaag tgttctggag tagtgtagtc
    14341 aatggggtgc tctggccgtg gattttcttg ctgatgcgta aagtccgtca gcagtttgca
    14401 gtgcaataaa ggtttctatg acttctctgt atttagcttc cggttctccg cgtcgtcagg
    14461 agttacttgc gcaacttggc gtgacctttg aacgtattgt tacgggcatt gaggagcagc
    14521 gtcagccgca ggagagcgcg cagcagtatg ttgtgcgtct ggcacgcgag aaagcacagg
    14581 cgggtgtcgc gcaaacggca caggatctcc cggtgctggg tgcggatatt atcgttatcc
    14641 tgaacggaga agtgctcgag aaaccgcgcg acgcagagca tgcggcgcag atgttgcgca
    14701 aattatcggg tcagacccat caggtgatga cggcagtggc gttggccgac agccagcaca
    14761 ttctcgattg cctggtggtc accgatgtga ctttcagaac gttaacagac gaagacatcg
    14821 cgggctatgt cgccagcggt gaaccgttag ataaagcagg tgcatacggt attcaggggc
    14881 tgggtggctg ttttgtcagg aagataaatg gcagctatca cgccgtagtc ggcttaccgc
    14941 tggttgaaac gtatgaatta ttaagtaatt ttaacgcact gcgtgagaaa agggataaac
    15001 atgacggctg aattgttagt aaacgtaacg ccttcggaaa cgcgagtggc gtatattgat
    15061 ggcggtattc tgcaggaaat tcatattgaa cgtgaggcgc gacgcggaat agtaggcaat
    15121 atctacaagg gtcgtgtaag tcgtgtactt ccgggtatgc aggcggcttt tgtagatatt
    15181 gggctggata aagccgcgtt tcttcatgca tccgacatca tgccgcatac cgaatgtgtg
    15241 gcgggtgaag aacaaaagca attcacggtg cgcgacatct cggaactggt tcgtcagggg
    15301 caagatctga tggtgcaggt ggtgaaagat ccgcttggca ctaaaggtgc gcgcctgacc
    15361 accgatatca cgctgccttc tcgctatctg gtatttatgc caggggcttc tcacgttggg
    15421 gtttcccaac gaattgaaag cgaatcagaa cgtgaacgtc tgaaaaaagt ggtcgcagag
    15481 tattgcgacg agcagggcgg gtttatcatc cgcaccgcag cggaaggggt tggcgaggct
    15541 gaactggcct ccgatgccgc ttatctgaaa cgcgtctgga ccaaagttat ggagcgtaaa
    15601 aagcgcccgc agacccgtta tcagctgtac ggcgaactgg cgctggcgca gcgtgttctg
    15661 cgtgattttg ccgacgccga actggaccgc attcgcgttg actcacgcct gacttacgaa
    15721 gcattgctgg agtttacctc ggagtacatt cccgagatga ccagcaagct ggagcattac
    15781 accggacgcc agccgatttt cgatctcttt gatgtcgaaa acgaaatcca gcgagcgttg
    15841 gaacgcaaag tagaactgaa atccggtggt tatctgatta tcgaccagac cgaagcgatg
    15901 accaccgtgg acatcaatac cggagcgttt gtcggtcatc gcaatctgga cgacactatt
    15961 ttcaatacca atattgaagc gacgcaggct atcgctcgcc agttacggtt gcgtaatctg
    16021 ggcgggatta tcattattga tttcatcgat atgaataatg aagatcaccg ccgccgcgtg
    16081 ctgcattcgc tggagcaggc gttgagcaaa gaccgggtga aaaccagcgt taatggtttt
    16141 tcggcgctgg ggctggtgga gatgacgcgt aaacgcaccc gcgaaagcat tgagcacgta
    16201 ctgtgtaacg aatgcccaac ctgccacggt cgcggaacgg tgaaaaccgt ggaaacggta
    16261 tgctatgaaa tcatgcgcga gattgttcgt gtccaccatg cttacgactc cgaccgtttc
    16321 ctggtctatg cttctccggc agtagccgaa gccttgaaag gcgaagagtc acactcgctg
    16381 gcggaagtgg aaattttcgt tggcaaacag gttaaagtac aaattgaacc gctctataac
    16441 caggagcagt ttgacgtcgt aatgatgtaa acagatgctg ggccgccatc cggcaaaggg
    16501 tttttgagtc acatttttag cagacaagga gtgacgggtg aggcgattgc cggggatttt
    16561 actgcttact ggagccgcgc tcgttgtgat cgctgccctg ctggttagcg gcctgcgtat
    16621 tgctttaccg catcttaacg cctggcgtcc ggaaatcctc aacaaaatag aatccgcgac
    16681 tggcatgccg gtagaagcca gtcagctctc agccagctgg cagaattttg gcccgacgct
    16741 tgaagcacac gacatccgtg cagaactaaa agatggcggc gaattttcgg ttaaacgcgt
    16801 tactctggcg ctggatgtct ggcagagcct gttacatatg cgctggcagt ttcgcgacct
    16861 cactttctgg cagctgcgct ttcgcaccaa cactcctatc accagcggtg gtagcgatga
    16921 cagtctggaa gccagtcaca tcagcgatct gtttcttcgt caatttgacc atttcgatct
    16981 ccgcgacagt gaagtcagtt ttctgacgcc atccggtcag cgcgccgagc tggcgatccc
    17041 acaactcacc tggctgaacg atccacgtcg acaccgtgcg gaaggcctgg taagcctctc
    17101 cagccttacc ggacagcacg gcgtgatgca ggtgcgcatg gatttgcgcg atgatgaggg
    17161 gttgttaagc aatggtcgcg tctggctaca ggcggatgac atcgacctga agccgtggct
    17221 cggtaaatgg atgcaggaca atattgctct ggaaacggca cagttctccc ttgaaggctg
    17281 gatgacgatc gacaaaggcg atgtaaccgg cggtgacgtc tggctgaaac agggcggtgc
    17341 cagctggttg ggcgagaagc aaacgcatac gctgtcggtg gataatctga ccgcgcatat
    17401 tacgcgtgaa aatccgggct ggcagttctc tattcccgat acacggatca cgatggacgg
    17461 caaaccctgg ccgagcggag cattgacgct ggcctggata ccggaacagg acgttggcgg
    17521 caaagacaat aaacgcagtg acgaactccg gattcgcgct agtaatctgg agctggcagg
    17581 cctggagggc atacgcccgc tggccgcgaa actttcacct gcactgggtg atgtttggcg
    17641 ctccacacaa ccgagcggca agattaacac tctggcgctg gatatcccgc ttcaggcggc
    17701 agacaagacc cgttttcagg catcgtggag cgatctggcc tggaagcaat ggaaattatt
    17761 accgggtgcg gaacacttct ccgggacgct ttccggcagc gttgaaaatg gtttgcttac
    17821 cgcgtcgatg aagcaggcaa agatgcctta cgaaacggta ttccgtgcgc cactggaaat
    17881 cgccgacggc caggcaacta taagctggct gaacaatgac aaaggtttcc agctggatgg
    17941 gcgtaatatt gacgttaaag ccaaagccgt ccatgcgcgc ggcggttttc gttacctgca
    18001 acctgctaac gatgaaccct ggctgggtat tctggctggc atcagtaccg atgatggttc
    18061 acaagcctgg cgctatttcc cggaaaactt gatgggtaaa gacctggttg attatttaag
    18121 tggtgcgatt cagggcggtg aagcggataa cgcgacgctg gtttatggtg gcaatccgca
    18181 actcttcccc tataaacaca acgaaggtca gtttgaagtg ctggtgccgc tgcgcaacgc
    18241 gaagtttgcc ttccagccgg actggcctgc attaactaac cttgatattg aactggactt
    18301 tattaacgac ggtttatgga tgaaaaccga tggcgttaat ctgggcggcg tgcgcgcgag
    18361 taatctcacc gcagtgatcc ctgactactc aaaagaaaaa ctgctgattg acgctgacat
    18421 taaaggtccg ggtaaagccg ttggccctta ctttgatgag acaccgctga aagattctct
    18481 gggtgcgacc ctgcaagaac tccagctcga cggcgatgtg aatgctcgct tacatcttga
    18541 tatcccgctg aacggcgaac tggtaaccgc gaaaggtgaa gtgacgctgc gtaataacag
    18601 tctgtttatc aaaccactcg acagcatcct gaaaaatttg agcggtaaat tcagctttat
    18661 caatggcgat ctgcaaagtg aaccactgac agcaagctgg tttaatcagc cgttgaacgt
    18721 ggatttttcc accaaagaag gggcaaaagc ctaccaggtt gcggtgaatc tcaacggtaa
    18781 ctggcaaccg gcgaaaaccg gcgttctgcc tgaagcggtg aacgaagcat tgagtggcag
    18841 cgtggcgtgg gatggtaaag tgggcattga tctgccttat catgctggcg cgacctataa
    18901 cgtagagctg aacggcgatc tgaataatgt gagtagtcac ttaccttcac cgttagccaa
    18961 acctgcgggt gaaccactag cggtaaacgt taaggttgat ggcaatctca acagctttga
    19021 attaaccgga caggctggtg cggataacca tttcaatagc cgctggttgc ttggtcaaaa
    19081 gctgacgctc gatcgtgcta tttgggcggc agacagtaaa acgctcccgc cgttgccgga
    19141 acaaagtggc gttgaactta atatgccgcc gatgaatggt gccgagtggc tggccctgtt
    19201 ccagaaaggc gctgcggaga gtgtcggtgg tgcagcgagt ttcccacaac acataacgtt
    19261 acgtacgcct atgttgtcac tgggaaatca gcaatggaat aacctgagta ttgtttcgca
    19321 accgacggca aatggcaccc tggttgaggc gcaagggcgt gaaatcaacg ccacgctagc
    19381 gatgcgtaat aacgcgccgt ggctggcgaa tatcaaatat ctttattaca acccgagcgt
    19441 ggcgaaaact cgtggtgatt caacgccgtc atcacctttc ccgacaacgg agcgcattaa
    19501 cttccgtggc tggccggacg cacaaatacg atgcacagag tgctggttct gggggcaaaa
    19561 attcggtcgc attgacagtg atatcaccat ttctggcgat acgttaacgc tgaccaatgg
    19621 actgattgat actggtttct cgcggcttac tgccgatggt gaatgggtta ataatccggg
    19681 gaatgaacgt acctcgctga aaggaaaact gcgtgggcag aaaattgatg ccgccgcaga
    19741 attttttggt gtcacgacgc ccatacgcca gtcgtcattt aatgtggatt acgatttaca
    19801 ctggcgcaaa gcaccctggc agccagatga agcgacgttg aatggcatca ttcatactca
    19861 actgggtaaa ggcgaaatta ccgaaatcaa taccggacat gccgggcaat tgctgcgctt
    19921 attgagcgta gatgccctga tgcgtaagct gcgttttgat ttcagagaca cttttggcga
    19981 agggttctat tttgactcca ttcgcagcac cgcgtggatt aaagacggcg ttatgcacac
    20041 cgacgacacg ctggtggatg gcctggaggc ggatatcgcc atgaaagggt cggtaaatct
    20101 ggtacgccgc gacctgaata tggaggcggt tgtcgcacca gagatttctg cgacggtggg
    20161 cgtggctgcg gcttttgcgg ttaaccccat tgttggcgcg gcagtgtttg ccgccagtaa
    20221 agtgctgggg ccgctgtgga gcaaagtctc cattttgcgc tatcacattt cgggtccgct
    20281 ggacgatccg caaatcaacg aagtgttgcg ccaaccgcgt aaagaaaaag cgcaatgatt
    20341 tgacgagggc gcgtaattgc cccaatctca tatgataatc gttgccaaag gccaacgagc
    20401 cggaacataa ctgtaggccg gatagggcgt tcacgccgca tccggcagcc gtaaaaaatc
    20461 ctctactgca gtaactaacg agtagcaaaa acgatgagtc tgaacctggt aagtgaacaa
    20521 ttgctagcgg cgaacggcct gaaacatcag gacttgttcg cgatcctcgg tcaactggcc
    20581 gaacgtcgcc ttgattatgg cgatctctat tttcagtcga gctatcacga atcctgggtt
    20641 ttagaagacc gcattattaa agatggttct tacaacatcg atcagggcgt tggcgtgcgt
    20701 gcaatcagcg gtgaaaaaac cggatttgct tacgctgacc aaatcagcct gctggcgctg
    20761 gaacagagtg cgcaagcggc gcgcaccatc gtccgtgata gtggtgatgg caaagtacag
    20821 acgctgggcg cggtagagca tagcccgttg tatacctcgg tagatccgct gcaaagcatg
    20881 agccgtgaag agaagctgga tatcctgcgt cgcgtcgata aggttgctcg cgaagcggac
    20941 aagcgcgtac aggaagtgac tgccagcctc agtggtgtct atgaattaat tctggttgcg
    21001 gccaccgacg gcacgctagc ggcggatgtc cgtccgctgg tgcgtctttc cgtgagcgtt
    21061 ctggtcgaag aagatggcaa acgcgaacgc ggtgccagtg gcggcggcgg tcgttttggt
    21121 tatgagttct tccttgccga tcttgacggc gaagtccgtg cggatgcatg ggcaaaagaa
    21181 gcagtgcgta tggcgctggt caatctttct gccgttgctg caccagcggg caccatgccg
    21241 gtagtacttg gcgcaggttg gccgggcgtg ctgttgcatg aagcggtagg tcacggtctg
    21301 gaaggcgact tcaaccgccg tggcacttca gtatttagtg gacaggtcgg ggagctggtg
    21361 gcttcagaac tgtgtaccgt ggttgatgac ggcacgatgg tcgatcgccg tggttcggtg
    21421 gcgattgatg atgaaggtac gccaggccag tacaacgtgc tgattgagaa cggcattctg
    21481 aaaggctaca tgcaggataa actcaacgcg cgtttgatgg ggatgacgcc gactggcaac
    21541 ggtcgccgtg aatcctacgc ccatctgccc atgccgcgta tgaccaacac ctatatgctg
    21601 ccgggtaaat cgaccccgca agaaattatt gaatccgttg agtacggtat ctatgcaccg
    21661 aactttggtg gcggtcaggt ggatatcacc tccggcaaat tcgttttctc cacttcagaa
    21721 gcatatctga ttgaaaacgg taaagtaacg aagccggtga aaggcgcaac gttgattggt
    21781 tccggtatcg aaaccatgca gcagatttcg atggttggca acgacctgaa actggataac
    21841 ggcgtgggtg tctgcggtaa agaagggcaa agtttgccgg ttggcgtggg ccagccaacg
    21901 ttgaaagtcg ataacctgac tgttggcggt actgcgtaat aatttcatta ttttcagaag
    21961 gataattaat ctttctacgt gcacgaacgg tcccctcgct cctctggggt tagggtgagg
    22021 ggaacccgtt ggcacaggtt tgtagaacgt aacagtacaa tatgaattac ttctctttcc
    22081 cgcgcccgtg catctcctga aacaatttac cgacctcaac aaaataatcc gtcagcgagt
    22141 tgatcacgac ctgtaccttc agcggcagct tatctttttc ggtatataac gcataaaccg
    22201 ggcgtggatc tgactgataa cgcggcagca ggatctccag ctccccacga ttgatctcgt
    22261 tgatcaccca catcagcggc acgtaggcga tcccggcacc cgccgttagc cagcgcacca
    22321 gcgtcatcgg atcattagtc acaaatctcc cttgcgggat caggcgagtc gagatccctt
    22381 ccggtgcaat cagttcaaat tcattgtcgg gccgcacgct gtattcaagc catgaatggc
    22441 tacttaaatc ggcgggtttt tccggtatgc cgtattgtgt gagatagctt ttcgcggcgc
    22501 acaccaccat tggcatcgcg ccaagacggc gggaaaacag gctggaatcc tgcaacgcgc
    22561 cgacgcggat caccacatcc agaccgtcgg caatcaggtc gggggctgga attccggtaa
    22621 ccagattggc gctcaaacct gggtattctt tcagcatttt ggctgtcagc ccggcgagaa
    22681 cattttgtgc catagttgaa gaacagccaa tgcgtagcgt cccgatgggg gtgttattga
    22741 aggcatacag ttgctcatga acatcctgca cttcatgaag catacgacgg cagccctggt
    22801 agtaaattct accggcttcg gtcaggccaa tgctgcgtgt gctacggttt aacagcttta
    22861 cctgcaactc atcttccagt tttgacaccg tctgactgat ggacgaaacg ctcatctgta
    22921 gctgtctggc ggcggcggta aaagagccaa attcaactac tttggcaaac accgacatgc
    22981 gttttagtcg ttccattatt cactctgact taaaagtgat ttagatcaca taatatagat
    23041 aacagcataa cagttacgct aatatattaa atatcaatct acagcaatgt tgctctcgcc
    23101 cggctttcca tgcccttctc tgcggcgaca gatgctgaaa ataataacgc ctgctctctc
    23161 tatacaacca aggtcaacat gagtctgttt cccgttatcg tggtgtttgg gctgtccttc
    23221 ccaccgatat tttttgaatt gcttttatca ctggcgattt tctggctggt gcgccgggta
    23281 cttgtgccaa caggtatcta cgactttgtc tggcatccgg cgttgttcaa caccgcgctc
    23341 tattgctgct tgttttattt gatatcgcga ctgttcgttt gaggttgaag tgaaaacact
    23401 aataagaaaa ttctcccgta cggccatcac ggtcgtatta gtcattctgg ccttcatcgc
    23461 aatttttaat gcctgggtct attacaccga atccccctgg acgcgtgacg cgcgctttag
    23521 cgctgacgtc gttgcgatcg cgccggacgt ttctggactc attacccagg tgaatgttca
    23581 tgataaccag ctggtgaaaa aaggacagat actgttcacc atcgaccagc cgcgctatca
    23641 aaaggcgctt gaggaagcgc aagccgatgt tgcttattat caggtactgg cacaggagaa
    23701 acgccaggag gccggacgtc gtaaccgtct cggtgtgcag gcgatgtctc gcgaagagat
    23761 cgaccaggct aacaacgtac tacaaacggt tctgcatcag ttagcgaaag cgcaggcgac
    23821 ccgcgatctg gcaaaactgg atcttgaacg cacggtgatc cgagcgccag cagatggctg
    23881 ggtgaccaac ctcaacgtct ataccggtga gtttattact cgaggatcaa cggcggttgc
    23941 gctggtgaaa cagaactcct tctatgtact ggcctatatg gaagaaacta agctggaagg
    24001 ggtgcgtccg gggtatcgtg cagagatcac gccgcttggc agtaacaaag tgctgaaagg
    24061 gactgttgat agtgttgccg ctggggtcac caacgccagc agcacgcgtg acgacaaagg
    24121 gatggcgacc atcgactctg accttgaatg ggtgcgtttg gcgcaacgtg ttccggttcg
    24181 tattcgtctc gacaaccagc aagagaacat ctggcctgcg ggcaccactg ctacagtggt
    24241 ggtcactggc aaacaagatc gcgacgaaag ccaggattcg ttcttccgta aaatggccca
    24301 tcgcctgcgt gagtttggtt aatcacgatg ggtattttct ccattgctaa ccaacatatt
    24361 cgctttgcgg taaaactggc gaccgccatt gtactggcgc tgtttgttgg ctttcacttc
    24421 cagctggaaa cgccacgctg ggcggtactg acagcggcga ttgttgccgc cggtccggcc
    24481 tttgctgcgg gaggtgaacc gtattctggc gctattcgct atcgtggctt tttgcgcatc
    24541 atcggcacat ttattggctg tattgccgga ctggtgatca tcattgcgat gatccgcgca
    24601 ccattattga tgattctggt gtgctgtatc tgggccggtt tttgtacctg gatatcctcg
    24661 ctggtacgaa tagaaaactc gtacgcgtgg gggctggccg gttataccgc gctgatcatt
    24721 gtgatcacca ttcagccgga accattgctt acgccgcagt ttgccgtcga acgttgtagc
    24781 gagatcgtta tcggtattgt gtgtgcgatt atggcggatt tgctcttttc tccgcgatcg
    24841 atcaaacaag aagtagatcg agagctggaa agtttgctgg tcgcgcaata tcaattaatg
    24901 caactctgta tcaagcatgg cgatggtgaa gttgtcgata aagcctgggg cgacctggtg
    24961 cgacgcacca cggcactaca aggcatgcgc agcaacctga atatggaatc ttcccgctgg
    25021 gcgcgggcca atcgacgttt aaaagcgatc aatacgctat cgctgacgct gattacccaa
    25081 tcctgcgaaa cttatcttat tcagaatacg cgcccggaat tgatcactga tactttccgc
    25141 gaattttttg acacgccggt agaaaccgcg caggacgtcc acaagcagct caaacgcctg
    25201 cggagagtta tcgcctggac tggggaacgg gaaacgcctg tcaccattta tagctgggtc
    25261 gcggcggcaa cgcgttatca gcttctcaag cgcggcgtta tcagtaacac aaaaatcaac
    25321 gccaccgaag aagagatcct gcaaggcgaa ccggaagtaa aagtagagtc agccgaacgt
    25381 catcatgcaa tggttaactt ctggcgaacc acactttcct gcattctggg cacgcttttc
    25441 tggctgtgga cgggctggac ttccggcagt ggtgcaatgg tgatgattgc ggtcgtgacg
    25501 tcactggcaa tgcgtttgcc gaatccacgc atggtggcga tcgactttat ctatgggacg
    25561 ctggccgcgc tgccgttagg gctgctctac tttttggtga ttatccctaa tacccaacag
    25621 agcatgttgc tgctgtgtat tagcctggca gtgctgggat tcttcctcgg tatagaagta
    25681 cagaaacggc gactgggctc gatgggggca ctggccagca ccataaatat tatcgtgctg
    25741 gataacccga tgactttcca tttcagtcag tttctcgaca gcgcattagg acaaatcgtc
    25801 ggctgtgtgc tcgcgttcac cgttattttg ctggtgcggg ataaatcgcg cgacaggact
    25861 ggacgtgtac tgcttaatca gtttgtttct gccgctgttt ccgcgatgac taccaatgtg
    25921 gcacgtcgta aagagaacca cctcccggca ctttatcagc agctgttttt gctgatgaat
    25981 aagttcccag gggatttgcc gaaatttcgc ctggcgctga cgatgattat cgcgcaccag
    26041 cgcctgcgtg atgcgccgat cccggttaac gaggatttat cggcgtttca ccgacaaatg
    26101 cgccgcacag cagaccatgt gatatctgcc cgtagcgatg ataaacgtcg tcggtacttt
    26161 ggccagttgc tggaagaact ggaaatctac caggaaaagc tacgcatctg gcaagcgcca
    26221 ccgcaggtga cggaaccggt tcatcggctg gcggggatgc tccataagta tcaacatgcg
    26281 ttgaccgata gttaagtcaa aaccgacgcc aaaagcgtcg gttttttcat ggctatactt
    26341 agcgatgcac ggcagaactc gccgcgaaac gtgacggtgg caacagatga atatttatgc
    26401 ctttgatttt gatgagattg agagtcagga ggatttttat cgtgacttta gccaaacctt
    26461 tggtctggct aaagataagg tacgcgatct cgactcacta tgggatgtgt taatgaacga
    26521 tgtcctgccg ctaccacttg agattgaatt tgttcatctg ggagagaaaa cgcgtcgccg
    26581 ttttggcgcg ttaatattgc tgtttgatga ggcagaggaa gagctggaag ggcatttgcg
    26641 ttttaatgtt cgtcattagc gcaaaaaaaa gcccacgaac cgggggcaat atcgtcggac
    26701 aaaacgatga gggtttattt gtacagttca gccgtagcgt gccaggtgtc accgctacga
    26761 gcttcagtaa tctggtaggc cgttgcgcct ttctcttccg cttttttgtt cagcatttca
    26821 cgtatatcca ttggcgaaga cgccacacca cttacggata cggtcccgat tgcttcacga
    26881 ttttgtgctt gtgcagcatc aatggagtcg gcagcgaatg caccgaaaga gagaacagaa
    26941 agtacgctta atgcagctac agtggttttg attttcatga tttttacctc gacataatct
    27001 tttagctggg cctttgtttc gtgacccata tcacaaaatc aagtatacac taattattga
    27061 actaattaat accacactaa ctgtttcagt tgatactaag tgttaaaaat gtgaatttta
    27121 ataacaaaaa agtataaaaa acacggttat ttattattaa ttatagtatt ttcaattaga
    27181 taatgaattt tgcataatgt gcttttttta ccgtatgtat tcattttgtg aatgatgtgt
    27241 cgcggtaaaa cgcactattc gttaaatgta tcgcaggaga aagaagggga atgagaggga
    27301 taagcaacat tttccccgcc gccagaagcg acggggcaga gattaaagct cctggtcgaa
    27361 cagctctaaa atcgcttcgt acaggtcttt gacggtgaaa ccgttagcag gggtggtaaa
    27421 gatggtgtca tcgccagcga tggtgcccag aataccttct gctttgccca gtgagtccag
    27481 caggcgagca attaactgcg ccgcgccagg gctggtatga atcacgacaa ctgcatcgtt
    27541 gtagtcgata tccagcacca gattcttcaa tggactggag gtggttggta cacccagttc
    27601 agctggcagg cagtaaacca tttccatttt ggcattgcgt gtacgtacag caccaaactt
    27661 ggtcaacatc cgcgagactt tagactgatt aatattgtca aagccttgct cctgcaacgc
    27721 ggcgacgatt tcgccctggg agctaaattt ctcttcttta agtaatgctt taaatgcttt
    27781 aactagttct tcttgcttag ccgagcttcg cataagtcac ccgatatggt ggttgataca
    27841 acattattgt gcatacagat gaatttttat gcaaacagtc agccctgaag aaggctgaaa
    27901 taatgttatg aaagagcggg attttatcaa atttcgttat tgagaaacat gcctgcgtca
    27961 cggcatgcaa attctgttta aaagtaaatt aattgttatc aaattgatgt tgttttggct
    28021 gaacggtagg gtatattgtc accacctgtt ggaatgttgc gctaatgcat aagcgactgt
    28081 taattacgta agttaggttc ctgattacgg caattaaatg cataaacgct aaacttgcgt
    28141 gactacacat tcttgagatg tggtcattgt aaacggcaat tttgtggatt aaggtcgcgg
    28201 cagcggagca acatatctta gtttatcaat ataataagga gtttaggatg aaagtcgcag
    28261 tcctcggcgc tgctggcggt attggccagg cgcttgcact actgttaaaa acccaactgc
    28321 cttcaggttc agaactctct ctgtatgata tcgctccagt gactcccggt gtggccgtcg
    28381 atctgagcca tatccctact gctgtgaaaa tcaaaggttt ttctggtgaa gatgcgactc
    28441 cggcgctgga aggcgcagat gtcgttctta tctctgcagg cgtagcgcgt aaaccgggta
    28501 tggatcgttc cgacctgttt aacgttaacg ccggcatcgt gaaaaacctg gtacagcaag
    28561 ttgcgaaaaa ctgcccgaaa gcgtgcattg gtattatcac taacccggtt aacaccacag
    28621 ttgcaattgc tgctgaagtg ctgaaaaaag ccggtgttta tgacaaaaac aaactgttcg
    28681 gcgttaccac gctggatatc attcgttcca acacctttgt tgcggaactg aaaggcaaac
    28741 agccaggcga agttgaagtg ccggttattg gcggtcactc tggtgttacc attctgccgc
    28801 tgctgtcaca ggttcctggc gttagtttta ccgagcagga agtggctgat ctgaccaaac
    28861 gtatccagaa cgcgggtact gaggtggttg aagcgaaagc cggtggcggg tctgcaaccc
    28921 tgtctatggg ccaggcagct gcacgttttg gtctgtctct ggttcgtgca ctgcagggcg
    28981 aacaaggcgt tgtcgaatgt gcctacgttg aaggcgacgg tcagtacgcc cgtttcttct
    29041 ctcaaccgct gctgctgggt aaaaacggcg tggaagagcg taaatctatc ggtaccctga
    29101 gcgcatttga acagaacgcg ctggaaggta tgctggatac gctcaagaaa gatatcgccc
    29161 tgggcaaaga gttcgttaat aagtaattga ttagcgaata ataaaaaacc ggagcacaga
    29221 ctccggtttt tgttttgagc actcgactta attggttgcc ggatattcct gaatggtgac
    29281 ctgcagcgtt aactgcttat catcacgcat cactacaacc gggatcaccg aaccagggcg
    29341 aatttccgcc acctgatcca tcgtctccag agcagagatg gccggtttgt tatccaccga
    29401 aataatcaga tcgttgacct gaatacccgc attcgccgct gggccgtcag gtgacacttc
    29461 attaaccacg atcccttgca gttgatctat accaccgctc tgcgcgtgca gtggtgcgat
    29521 ctcgcgtccg ccgataccaa tgtagccgcg gatcacgcga ccatcgcgga tcagcttatc
    29581 cataattttg gttgctaact ggaaaggaat cgcaaagccg ataccttccg gcgtttcgcc
    29641 atcgttactc ttatcaaacg acagcgtgtt aatgcccatc agttctccca gcgagttcac
    29701 cagcgcgccg ccagagttac cgtggttaat ggaagcatcg gtttgtagga agttttgccg
    29761 cccggtcggg ttcagaccga ttcgacccgt ggcactaata atcccttggg taatggtctg
    29821 cccgaggttg tacgggttac cgatcgccag tactacgtcg ccaatgtgcg gtacgcgacg
    29881 tgcattaatt ggaatggtag gtaaaccgcc agtggcatta attttaagta ccgccagatc
    29941 ggttagagag tcagatccca ccagcaatgc ttcaaataca cgtccatcct gtaaggcgac
    30001 gatgatctga tcggcgtcgt tgatgacgtg tttattggtg atgatataac cgcgttgatc
    30061 catgattaca ccggatccca gggtgcggat ctcaagctgg ttgtgagagt tggtgttcaa
    30121 accacggttg taaacgttaa ccaccgctgg cgcggcgcgg cgaaccgcca gattatagct
    30181 ggcaggcgtc tcatcggtac tgtcaaattg cggagtggaa agcgggttaa ggctgcgcag
    30241 cgaaggcatg gcaaccagca gaatagcgcc gacaattaat ccaatcgcaa cggaacgtaa
    30301 gagcttcaca aacatgatgg aggcgtcatt aaaaaaggga acggcagcag cataccacga
    30361 gttaaccgga catcacacgt cagcctgatg cccggtttac gacattaacg catcagcaga
    30421 tagatgcttt cattgccgcg tacaatttgc agggcgatga tggccggttt tgccgccagc
    30481 actttacgca tttcagcaat cgagttcacc cgatcgcggt tgacgccaat gatcacatcg
    30541 tctttttgca agccagcctg agcagctggg cttcctttga caacttcatc tatcttaata
    30601 cctttgccgc catcttttag ctgaccatcg ctcaacgttg caccttccag cgctggccga
    30661 cgaagaggtg ctggtatcaa gcgtcacttc tacttccagt ggtttgccgt tacgcagcag
    30721 gccaagcttc actttcgtgc ccggctctgt agtcgcgata cgagagcgca actcagcaaa
    30781 gctattcagc ggtttgccgt tgaggctggt aataatatcg cccgctttga cgcccgcttt
    30841 cgctgagcca gaacttggca acacttcgct gacaaacgcg ccacgctgca cgtcaaggtt
    30901 gaaggctttg gcgatatcgg cactcatctc ggtgcctttg atgcctaaca aaccgcgttt
    30961 gatttcacca aagtcgataa gctgctgcgc cagtgttcgc gccatattac tggggatggc
    31021 aaatccaatc ccgacgctcc cgccgccagg cgcaaggatt gcagtgttga tgccaattaa
    31081 ctcaccgtta aggtttaata gtgcaccgcc ggagttaccg cggttaatgg aagcatctgt
    31141 ctggataaag ttttccagac cttcaagatt caacccgctg cggcctaatg cggaaacaat
    31201 gccagaggtg gcggtttgcc caaggccaaa tgggttaccg accgctacgg caaaatcacc
    31261 gacgcgcaat ttatcggagt cggcaatagc gatttgcgtt aatttgctcg ggttttgaat
    31321 ttgtaacagg gcgatatcgc tctggtcatc gctaccaatc agttttgcat caaactcgcg
    31381 cccatcattg agctgaatac tgattttctg tgcctgatta atcacatggt tgttggtcag
    31441 cacatagcct ttactggcgt tgatgatgac accggaacct aaaccttcga agggttgtgc
    31501 aggttgatcc ggtaaatcat caccaaaaaa ctttttgaat tcttccggga ttttctgtcc
    31561 ctgactggcc gttccttcca cccgtacgct caccactgcc ggaagcactt tttccagcat
    31621 tggagccaga ctggggagag gggcctgatc ggcaacctgg cctggaatcg acgcgacggc
    31681 ctgaaatgac gccgagagag ttaacccggc acttaacgct aatgcactca acagctgggt
    31741 ttgttttttc attattcctg ctctcgtacc tgaatgataa gaaaagagat tcaaaacagt
    31801 ttgatgttat tgaattttca gacggctaac aatgagacac cagattaaat gatagtcggg
    31861 gagggagaga agaggagggc gcaatggctg cgcccgaaaa ataaattagt cgcgcttcgc
    31921 gccagtacgc agcaggccgg atgcgccttc agaatagtcg cgaggcacct gcaccggtgc
    31981 ctgatcgttg ctggcttcag actctgccag acgattacgg aacgggtttg cttcagcaga
    32041 cagttccggc agcaggctgc tggagctttt tgccatgtgc tgatacagct ggcgatagtc
    32101 gtgcgccatg gtatccagta attccgcgct gcgggcaaag tggctaacca gctcttcgcg
    32161 atactcgtcc agttcagctt tattcttttc cagttcgtac tgcaacgcct gttgctggcg
    32221 tagtttacga ttaccaaaac gcatggccac agcaccaata atgatgccga cgactaaccc
    32281 aattagcgca tattcccagg tcatgaacat ctcccgttgt cttttgtttc cgtagggtgt
    32341 tggcttcagg ctccatgcct gcggctgatt atgccactat aaccgttaat tccacagaag
    32401 tggaatcccg actgcatatc gcgtagtgta gaacggcctt tttttcgtca accgtgaaca
    32461 acggcgcacc gattattcaa ggaataacaa taagatcatg caaagcgtta ccccaacatc
    32521 gcaatacctg aaggcgctca atgaaggcag ccatcaaccc gacgacgttc aaaaagaggc
    32581 cgtcagccgc ctggaaatta tttatcaaga actcatcaat agcaggccac cagcccccag
    32641 gacgagtggg ctaatggcgc gggtcggtaa gctgtggggt aaacgcgaag acacaaagca
    32701 tacgccagtg cgtggcttat atatgtgggg cggtgtagga cgcgggaaaa cctggctgat
    32761 ggaccttttc tatcaaagcc tgccgggaga gcggaaacag cgcctgcact ttcaccgttt
    32821 tatgctgcgg gtgcacgaag agctaactgc cttacagggg cagaccgatc cgctggaaat
    32881 tattgccgat cgctttaaag ccgaaactga cgtgctctgt tttgacgaat tttttgtttc
    32941 tgatattacc gacgccatgc tacttggcgg tctgatgaaa gccctgttca cccgcggtat
    33001 taccctggta gcgacgtcaa atattccgcc ggacgaactt tatcgaaatg gcctgcaacg
    33061 tgcgcgtttt ctgcctgcaa tcgatgccat taaacagcat tgtgatgtaa tgaacgtgga
    33121 cgctggtgtt gattatcgac tgcgtacact cactcaggcg catctgtggc tttcgccact
    33181 caacgatgaa acccgggcgc agatggataa actatggttg gcgctggcgg gggtgaaacg
    33241 agaaaattca ccgacgttag aaatcaacca tcggccattg gcgacaatgg gcgtcgagaa
    33301 ccagacgctg gcggtttctt ttactacgct gtgcgtcgac gcccgcagtc agcatgacta
    33361 tattgcgctc tcacgcctgt ttcacacagt aatgttgttt gatgtgccag tcatgacgcg
    33421 gttgatggag agcgaagcgc ggcgctttat tgcgctggtg gatgagtttt acgagcgcca
    33481 tgtcaaatta gtggtgagtg cagaagtgcc gctgtatgaa atttatcagg gtgagcggct
    33541 gaagtttgag ttccagcgtt gcctgtcacg tctgcaagag atgcaaagcg aagagtatct
    33601 gaagcgcgag catttggcgg gttaaaacct gccacaaatc acaaaaaggg gtcgatcttt
    33661 gaccccgact tctctataat cctgcgaccc cacgttacaa gaaagttttt ttcccaaaac
    33721 tttttgtgtg ctggcatagg ctattcgaag gggtaggttt gccggacttt gtcgtgtgaa
    33781 cctcaacaat tgaagacgtt tgggtgttca ccaacgtgta actatttatt gggtaagctt
    33841 ttaatgaaaa cttttacagc taaaccagaa accgtaaaac gcgactggta tgttgttgac
    33901 gcgaccggta aaactctggg ccgtctggct actgaactgg ctcgtcgcct gcgcggtaag
    33961 cacaaagcgg aatatactcc gcacgtagat accggtgatt acatcatcgt tctgaacgct
    34021 gacaaagttg ctgtaaccgg caacaagcgt actgacaaag tgtactatca ccacaccggc
    34081 cacatcggtg gtatcaaaca agcgaccttt gaagagatga ttgctcgccg tcctgagcgt
    34141 gtgattgaaa tcgcggttaa aggcatgttg ccaaaaggcc cgctgggtcg tgctatgttc
    34201 cgtaaactga aagtttacgc gggtaacgag cacaaccacg cggcacagca accgcaagtt
    34261 cttgacatct aatcgggatt ataggcaatg gctgaaaatc aatactacgg cactggtcgc
    34321 cgcaaaagtt ccgcagctcg cgttttcatc aaaccgggca acggtaaaat cgtaatcaac
    34381 caacgttctc tggaacagta cttcggtcgt gaaactgccc gcatggtagt tcgtcagccg
    34441 ctggaactgg tcgacatggt tgagaaactg gacctgtaca tcaccgttaa aggtggtggt
    34501 atctctggtc aggctggtgc gatccgtcac ggtatcaccc gcgctctgat ggaatacgac
    34561 gagtccctgc gttctgaact gcgtaaagct ggcttcgtta ctcgtgacgc tcgtcaggtt
    34621 gaacgtaaga aagtcggtct gcgtaaagca cgtcgtcgtc cgcagttctc caaacgttaa
    34681 ttggcttctg ctacggcaga aaacaatttg cgaaaaaacc cgcttcggcg ggttttttta
    34741 tggctaaatt ctgaatcagc gtaaaaaatg ggaagttgct ttttgctgcc gtctgaaaga
    34801 caggtaaaac aaaaaccaca tcgccaataa gggtctaagt caactatttc agactaaagc
    34861 gcatctcttt ttccccattt ccggcatcca ctcaccacaa aggtcgcaaa atctggtaaa
    34921 ctatcatcca attttctgcc caaatgtcgg gtattgctca ttttttgttt gattttcgaa
    34981 caaagagagt cgttccttat tgggtaacac aacttctgac tggccacctg gtggctggta
    35041 gcagtaaaaa ttctgactat acctggaggt tttcatggct gtcgctgcca acaaacgttc
    35101 ggtaatgacg ctgttttccg gtcctactga catctatagc catcaggtcc gcattgtgct
    35161 ggctgagaaa ggtgtaagtt tcgagatcga acacgtggaa aaggacaatc cgcctcagga
    35221 tctgattgac ctcaacccga atcagagcgt tccgaccctg gtggatcgtg agctgaccct
    35281 gtgggaatct cgcatcatta tggaatatct ggatgagcgt ttcccgcatc cgccactgat
    35341 gcctgtttac ccggtagctc gcggtgaaag ccgtctgtac atgcatcgca tcgaaaaaga
    35401 ctggtacacg ctgatgaaca ctatcatcaa cggttcagct tctgaagcag atgccgcacg
    35461 taagcaactg cgcgaagaac tgctggcgat tgcgccggtc ttcggtcaga agccgtactt
    35521 cctgagcgat gagttcagcc tggtcgattg ctaccttgct ccgctgctgt ggcgtctgcc
    35581 gcaactgggc atcgagttca gcggcccggg tgcgaaagag ctgaaaggct atatgacccg
    35641 cgtctttgag cgtgactctt tccttgcttc tttaactgaa gcagaacgtg aaatgcgtct
    35701 gggccggagt taatctgtat ggatttgtca cagctaacac cacgtcgtcc ctatctgctg
    35761 cgtgcattct atgagtggtt gctggataac cagctcacgc cgcacctggt ggtggatgtg
    35821 acgctccctg gcgtgcaggt tcctatggaa tatgcgcgtg acgggcaaat cgtactcaac
    35881 attgcgccgc gtgctgtcgg caatctggaa ctggcgaatg atgaggtgcg ctttaacgcg
    35941 cgctttggtg gcattccgcg tcaggtttct gtgccgctgg ctgccgtgct ggctatctac
    36001 gcccgtgaaa atggcgcagg cacgatgttt gagcctgaag ctgcctacga tgaagatacc
    36061 agcatcatga atgatgaaga ggcatcggca gacaacgaaa ccgttatgtc ggttattgat
    36121 ggcgacaagc cagatcacga tgatgacact catcctgacg atgaacctcc gcagccacca
    36181 cgcggtggtc gaccggcatt acgcgttgtg aagtaataca aaacaggccc aggcggcctg
    36241 ttttgtcttt ttaatgactt aataaaatag cgtgattgtg gcaatcatat tcaccacgaa
    36301 accgactgcc atggggatcg ctgtccgctt caccaccgca aaaggcgaaa cgcctgcaat
    36361 tcctgacgta acgaccacca ccgcagtaat cggcgaaacc gcgcgcgcta gcgtcgtggc
    36421 aaaatgcatc ggcataatca ttacaaccgc tggtacatgt agtccggctg cgatattcgg
    36481 aataagactg gcaaatgaca taaacggcgc attgccagag cccatcacaa tggcacaaat
    36541 ggcaatgact agcgccataa taatcatcac gccaataccg cccagaccag aatgctccgc
    36601 acccctgata accgcatcaa cagtgccaat cgtcgttaag cctttcgcaa atatttcacc
    36661 cgcgaccacc agcgttacca cgttgacaaa ctgcgtaccc atgccgtcaa aaaacgcctg
    36721 cacatcgtcc attgtttcgc gcaagttatg cttgcggaag aactcaacaa acatcgtcac
    36781 cgtcaaactt agtaacatca ccaccaccag atgcagttct gactgcatca gcccgacgtg
    36841 ggcgaggaac agcgagccga gcatcaggat taacggcatc acaggtaaaa tggcgtaata
    36901 gagcggcggg acattatcga gagttttttg ctctgcctgt tcgtgattga tatttttatc
    36961 ttttttgtca aaagcgcgtt gcacgaaaaa gtgggagatc gccaccgaga taatgacgca
    37021 agaggcgacc ggaagctggt agtggaagaa gtatgtggca attttcattc ccgcgacctg
    37081 ggctgcaaaa atggagttcg tttccagaat cccccactca atggacatcg tggttgcgat
    37141 aaccgccacc gcagagaggc gacttactcc cagactcacc agcgtcggaa ataaggtgac
    37201 catcagcaac atacccagac cggaggcgct ggtaataaac tgcgccatga tttggccgat
    37261 gacgtaagtt gccgacagaa taatatacgg cgagcgaatg agttttaacg ggcggcttag
    37321 caagctcacc atcgcgcaac tggctcctat gcgctccatg tagcgggcat aaccgcccac
    37381 cgccataatc gacagcccca atccggcaat gcggttggac agcatgcgca ggatttcatt
    37441 ataaatgtcg acgatcaagt agccgctgct tttggcggga tcgagtacac caccgaaccc
    37501 taaccagact ccgcacatca tcaggaagac acctgcggca gccagcacca cctgaggttt
    37561 gtagtttttc aggatcaaat agcccatcgt aattaatacg atgacagata taattatgcc
    37621 gaacatt
//
LOCUS       DAJTSI010000020        36070 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000020 DAJTSI010000000
VERSION     DAJTSI010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36070
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.020"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..565
                     /gene="gltI"
                     /locus_tag="OO327_000944"
     CDS             <1..565
                     /gene="gltI"
                     /locus_tag="OO327_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR6017578.1"
                     /translation="NNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVT
                     SGTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAK
                     KPDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIP
                     PKNLNMNFELSDEMKALFKEPNDKALN"
     gene            735..1475
                     /gene="gltJ"
                     /locus_tag="OO327_000945"
     CDS             735..1475
                     /gene="gltJ"
                     /locus_tag="OO327_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCR6017579.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            1475..2149
                     /gene="gltK"
                     /locus_tag="OO327_000946"
     CDS             1475..2149
                     /gene="gltK"
                     /locus_tag="OO327_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR6017580.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            2149..2874
                     /gene="gltL"
                     /locus_tag="OO327_000947"
     CDS             2149..2874
                     /gene="gltL"
                     /locus_tag="OO327_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR6017581.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            2992..3927
                     /gene="rihA"
                     /locus_tag="OO327_000948"
     CDS             2992..3927
                     /gene="rihA"
                     /locus_tag="OO327_000948"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR6017582.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            4011..5681
                     /gene="hscC"
                     /locus_tag="OO327_000949"
                     /pseudo
     CDS             4011..5681
                     /gene="hscC"
                     /locus_tag="OO327_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(5741..7192)
                     /gene="djlC"
                     /locus_tag="OO327_000950"
     CDS             complement(5741..7192)
                     /gene="djlC"
                     /locus_tag="OO327_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCR6017583.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(7189..7896)
                     /locus_tag="OO327_000951"
     CDS             complement(7189..7896)
                     /locus_tag="OO327_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6017584.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            7998..8552
                     /locus_tag="OO327_000952"
     CDS             7998..8552
                     /locus_tag="OO327_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6017585.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(8562..9989)
                     /locus_tag="OO327_000953"
     CDS             complement(8562..9989)
                     /locus_tag="OO327_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR6017586.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(9986..10693)
                     /locus_tag="OO327_000954"
     CDS             complement(9986..10693)
                     /locus_tag="OO327_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6017587.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            10857..11834
                     /locus_tag="OO327_000955"
     CDS             10857..11834
                     /locus_tag="OO327_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR6017588.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(11904..12386)
                     /locus_tag="OO327_000956"
     CDS             complement(11904..12386)
                     /locus_tag="OO327_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR6017589.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            12621..15203
                     /gene="leuS"
                     /locus_tag="OO327_000957"
     CDS             12621..15203
                     /gene="leuS"
                     /locus_tag="OO327_000957"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR6017590.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            15218..15799
                     /gene="lptE"
                     /locus_tag="OO327_000958"
     CDS             15218..15799
                     /gene="lptE"
                     /locus_tag="OO327_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR6017591.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            15799..16830
                     /gene="holA"
                     /locus_tag="OO327_000959"
     CDS             15799..16830
                     /gene="holA"
                     /locus_tag="OO327_000959"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR6017592.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            16832..17473
                     /gene="nadD"
                     /locus_tag="OO327_000960"
     CDS             16832..17473
                     /gene="nadD"
                     /locus_tag="OO327_000960"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR6017593.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            17497..18108
                     /locus_tag="OO327_000961"
     CDS             17497..18108
                     /locus_tag="OO327_000961"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCR6017594.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            18368..18685
                     /gene="rsfS"
                     /locus_tag="OO327_000962"
                     /gene_synonym="rsfA"
     CDS             18368..18685
                     /gene="rsfS"
                     /locus_tag="OO327_000962"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR6017595.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            18689..19156
                     /gene="rlmH"
                     /locus_tag="OO327_000963"
     CDS             18689..19156
                     /gene="rlmH"
                     /locus_tag="OO327_000963"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR6017596.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            19187..21088
                     /gene="mrdA"
                     /locus_tag="OO327_000964"
     CDS             19187..21088
                     /gene="mrdA"
                     /locus_tag="OO327_000964"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR6017597.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            21091..22203
                     /gene="mrdB"
                     /locus_tag="OO327_000965"
                     /gene_synonym="rodA"
     CDS             21091..22203
                     /gene="mrdB"
                     /locus_tag="OO327_000965"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR6017598.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            22214..23302
                     /gene="rlpA"
                     /locus_tag="OO327_000966"
     CDS             22214..23302
                     /gene="rlpA"
                     /locus_tag="OO327_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR6017599.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            23441..24652
                     /gene="dacA"
                     /locus_tag="OO327_000967"
     CDS             23441..24652
                     /gene="dacA"
                     /locus_tag="OO327_000967"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR6017600.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            24763..25026
                     /locus_tag="OO327_000968"
     CDS             24763..25026
                     /locus_tag="OO327_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR6017601.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            25127..25768
                     /gene="lipB"
                     /locus_tag="OO327_000969"
     CDS             25127..25768
                     /gene="lipB"
                     /locus_tag="OO327_000969"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR6017602.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGVEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            26027..26980
                     /locus_tag="OO327_000970"
     CDS             26027..26980
                     /locus_tag="OO327_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR6017603.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            27189..28154
                     /gene="lipA"
                     /locus_tag="OO327_000971"
     CDS             27189..28154
                     /gene="lipA"
                     /locus_tag="OO327_000971"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR6017604.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(28255..28458)
                     /gene="tatE"
                     /locus_tag="OO327_000972"
     CDS             complement(28255..28458)
                     /gene="tatE"
                     /locus_tag="OO327_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR6017605.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(28587..29375)
                     /locus_tag="OO327_000973"
     CDS             complement(28587..29375)
                     /locus_tag="OO327_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR6017606.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            29468..29851
                     /gene="crcB"
                     /locus_tag="OO327_000974"
     CDS             29468..29851
                     /gene="crcB"
                     /locus_tag="OO327_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR6017607.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIEPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(29905..30114)
                     /gene="cspE"
                     /locus_tag="OO327_000975"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(29905..30114)
                     /gene="cspE"
                     /locus_tag="OO327_000975"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6017608.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(30289..30849)
                     /gene="pagP"
                     /locus_tag="OO327_000976"
     CDS             complement(30289..30849)
                     /gene="pagP"
                     /locus_tag="OO327_000976"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR6017609.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            31438..32823
                     /gene="dcuC"
                     /locus_tag="OO327_000977"
     CDS             31438..32823
                     /gene="dcuC"
                     /locus_tag="OO327_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6017610.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(32864..33205)
                     /locus_tag="OO327_000978"
                     /pseudo
     CDS             complement(32864..33205)
                     /locus_tag="OO327_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(33543..34052)
                     /locus_tag="OO327_000979"
     CDS             complement(33543..34052)
                     /locus_tag="OO327_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6017611.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            34215..34460
                     /locus_tag="OO327_000980"
     CDS             34215..34460
                     /locus_tag="OO327_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6017612.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            34435..36066
                     /locus_tag="OO327_000981"
                     /pseudo
     CDS             34435..36066
                     /locus_tag="OO327_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
BASE COUNT         9169 a         8864 c         8908 g         9129 t
ORIGIN      
        1 caacaacgtc gaacgccaaa aacaggcggc tttctctgac actattttcg tggtcggtac
       61 gcgcctgttg accaaaaagg gcggcgatat caaagatttt gccgacctga aaggcaaagc
      121 cgtagtcgtc acttccggca ctacctctga agttttgctc aacaaactga atgaagagca
      181 aaaaatgaat atgcgcatca tcagcgccaa agatcacggt gactctttcc gcaccctgga
      241 aagcggtcgt gccgttgcct ttatgatgga tgacgctctg ctggccggtg aacgtgcgaa
      301 agcgaagaaa ccagacaact gggatatcgt cggcaagccg cagtctcagg aagcctacgg
      361 ttgcatgttg cgtaaagacg atccgcagtt caaaaagctg atggatgaca ctatcgctca
      421 ggtgcagacc tccggtgaag cggaaaaatg gtttgataaa tggttcaaaa atccaattcc
      481 gccgaaaaac ctgaacatga atttcgaact gtcagacgaa atgaaagcac tgttcaaaga
      541 accgaatgac aaggcactga actaattaca agaaccaggg gcggaaattc cagccctctc
      601 gattgttacg tagcacggac agactatacg cctgatggtc gttccccatc gggcctgaaa
      661 accgcaatac gctgggtaac aatcttcgag ggtagcagtt aacgctgcta ccctcttttt
      721 tctggagtag atttatgtct atagactgga actggggtat ttttttacaa caagccccgt
      781 tcggcaatac cacctatctt ggttgggtct ggagtggttt tcaggtgacg atcgctttgt
      841 cgatctgcgc ctggattatc gctttcctcg tcggttcatt tttcggcatt ttacgtaccg
      901 ttccaaaccg ttttctctcc ggtctgggta cgttgtatgt cgaactgttc cgcaacgtgc
      961 cgttgattgt gcaattcttt acctggtatc tggtgatccc ggagctgctg ccggagaaaa
     1021 tcggcatgtg gtttaaggcc gagctggatc ccaatattca gtttttcctt tcatccatgc
     1081 tctgcctggg gctgtttacc gccgcccgtg tttgcgaaca ggtacgcgcc gctattcagt
     1141 cgctaccgcg tgggcagaag aatgccgcgc tggcgatggg cctgacgcta ccgcagactt
     1201 atcgatacgt actgctgcct aatgcttatc gcgttatcgt cccgccgatg acttccgaga
     1261 tgatgaacct ggtgaaaaac tccgccatcg cctcgaccat cggtctggtg gatatggcgg
     1321 cgcaggcggg taaattgctc gactactcgg ctcacgcatg ggagtcgttt accgccatca
     1381 cgcttgcgta tgtgttgatt aacgctttta tcatgctggt gatgacgctg gttgaacgta
     1441 aagttcgcct gcccggcaac atggggggca aataatgtac gagtttgact ggagttccat
     1501 tgttccttcc ctgccatatc tgctcgacgg gctggtgatc accctgaaaa tcaccgtcac
     1561 ggcagtcgtg attggtattt tgtggggtac catgctggcg gtgatgcgct tgtccagctt
     1621 cgcgcctgtt tcgtggtttg ccaaagccta cgttaacgta ttccgctcga ttcctttagt
     1681 catggttttg ctgtggtttt acttgattgt gccgggattt ctgcaaaatg tgctgggatt
     1741 atcgccaaaa aatgatattc gtttaatttc ggcgatggtg gcgttttcga tgtttgaagc
     1801 ggcctactat tcagagatta tccgtgccgg tattcaaagt atttcccgtg gtcagtcgag
     1861 cgccgcgctg gcgttgggga tgactcactg gcagtcgatg aaactgatta ttctgccgca
     1921 ggctttccgc gcgatggtgc cgctgctgct cactcagggc atcgtactgt tccaggatac
     1981 ctcgctggtg tatgtgttat ctctggccga tttcttccgt accgcctcaa ccattggtga
     2041 gcgtgatggt acgcaggtcg agatgatcct gtttgccgga tttgtttatt tcgttattag
     2101 ccttagcgcg tcgttgttgg tcagctactt gaaaagaagg acagcataat gattaccctg
     2161 aaaaatgttt caaaatggta tggtcacttt caggtgctga ccgactgctc aaccgaagtg
     2221 aaaaaaggcg aagtggtggt ggtttgcggc ccgtctggtt ccggcaaatc aacgctgatt
     2281 aaaaccgtca acggcctcga accagtgcag caaggtgaaa tcaccgtcga tggtatcgtg
     2341 gttaacgaca agaaaaccga tctggcaaag ctgcgctccc gcgtcgggat ggtattccag
     2401 catttcgagc tgttccctca tctgtcgatt atcgaaaacc tgaccctggc gcaggtgaaa
     2461 gtgcttaaac gcgataaagc gcccgctcgt gaaaaagcat tgaaattgct ggagcgcgtg
     2521 ggactttccg cacatgccaa taaatttccg gcgcagcttt ccggcggtca gcagcagcgc
     2581 gtggcaatcg ctcgcgcatt gtgtatggct cctattgcga tgctgttcga cgaaccgaca
     2641 tcggcgctgg atccggagat gatcaacgaa gtgctggacg tgatggtgga actggcgaac
     2701 gaaggaatga ccatgatggt ggtgacccac gaaatgggct ttgcccgcaa agtggcgaat
     2761 cgggtgatct ttatggacga gggtaaaatt gtcgaagact cgccgaaaga cgctttcttc
     2821 gatgatccga aatcggaccg cgcaaaagac ttcctcgcga aaatcctgca ttaatcactc
     2881 tggcgcacat acccaattgc gcgccatctc acgctttatc gtttcgcccc tcgtatctgt
     2941 tacatcctgg cgttaccctt gtcgcaaaga agcacacaac aaggagcaac aatggcactg
     3001 ccaattctgt tagattgcga cccaggtcat gatgacgcta tcgcaatagt tctcgcactc
     3061 gcctcaccag agcttgatgt caaagcaatt acgtcttccg ccggaaacca gacaccagaa
     3121 aaaaccttac gcaatgttct gcgtatgctg accttgctta atcgcaccga tattccggtg
     3181 gcaggcggcg cggtaaaacc gttaatgcgt gagttgatta tcgcggacaa tgtgcacggc
     3241 gaaagcggtc tcgacggccc ggcattaccg gaaccgacgt tcgcaccgca aaactgtacg
     3301 gcggtggagc tgatggcgaa aacgctgcgc gaaagtgcgg aacctgtcac gattgtttct
     3361 accggaccgc aaactaacgt tgccttgctg ctcaatagcc atccggaact gcatagcaaa
     3421 attgcccgta tcgtgattat gggcggcgca atggggcttg gtaactggac gcctgcggct
     3481 gaatttaata tttacgttga cccggaagcg gcagaaattg tcttccagtc agggatcccg
     3541 gtggtgatgg ccggtctgga tgttactcat aaagcgcaaa tccacgttga agacaccgag
     3601 cgtttccgcg cgattggcaa ccctgtttca accattgttg ccgaactgct ggatttcttc
     3661 ctcgaatatc ataaagacga aaaatggggc tttgtcggcg caccgctgca tgacccatgc
     3721 accatcgcct ggctgttgaa acccgagttg tttaccactg ttgagcgctg ggttggcgtg
     3781 gaaacacagg ggaaatatac ccagggtatg acggttgttg attattatta tctgactggc
     3841 aataaaccga atgccactgt aattgtcgat gttgatcgtc agggctttgt tgatttactg
     3901 gctgatcgtc tgaaatttta cgcttaacac tcgtcattga ttaagatcac cacgcgttac
     3961 ctgggtggtc ttaatctatt tatctctttt tctttcaaga tataaatgca atggataatg
     4021 cagaactcgc cataggtatc gatctcggca ctaccaatag tttaattgcc gtctggaaag
     4081 acggtgccgc gcaattaatt ccaaataagt tcggtgaata tttaacacca tccataatta
     4141 gcatggatga aaataatcat attttagtcg gaaaaccggc tgtatcacgg cgtacttcgc
     4201 atccggataa aacggcagcg ttatttaaac gtgcaatggg cagtaatacc aactggcggt
     4261 taggcagcga cacatttaac gcgccagaac tgtcctcttt ggtattacgc tcattaaaag
     4321 aagatgccga agaatttctg caacgtccga ttaaagatgt ggtgatctcc gttctggctt
     4381 atttcagcga tgaacaacgc aagcataccc gtttagcagc ggacttagcc gggttaaatg
     4441 cggtacgctt aattaatgaa cccacagcag ctgcgatggc gtatggcctg catacccaac
     4501 aaaatacccg ttcgctggtg tttgatctcg gtggcggcac gtttgacgtt acggtgcttg
     4561 agtacgccac gccggtgatt gaagttcacg cctccgctgg cgacaacttc ctcggtggcg
     4621 aagattttac ccatatgttg gtcgatgacg ttttaaagcg cgcggatgtc acaaggacca
     4681 cgcttaacga gagtgaactg gcagccttgt acgcctgtgt ggaagcggca aaatgtagca
     4741 atcaatcgcc aatgcacatt cgctggtagt accaggatga aacgcgggaa tgcgaatttt
     4801 acgagaacga actggaagat ttgtggttgc cgctgctcaa tcgcttgcga gtgccgattg
     4861 aacaggcgtt gcgcgatgcg cgtctgaagc cgagtcaaat cgacagtctg gtgctggttg
     4921 gcggcgcgtc acaaatgccg ctggtgcagc gaatcgccgt gcgtctgttt ggcaaattac
     4981 cgtatcaaag ttacgatccg agcaccattg tcgcgctggg cgcagcaatc caggccgcct
     5041 gccgcttacg cagtgaagat attgaagaag tgatcctcac tgatatttgc ccttactcgt
     5101 tgggcgttga ggttaaccgc cagggcgttt ccggcatttt ctcgccgatt attgaacgaa
     5161 acaccactgt gcccgtgtcg cgtgtagaaa cttattcaac catgcacccg gaacaggatt
     5221 caattacggt taacgtctat cagggagaaa accacaaagt taaaaacaac attctggtgg
     5281 aatccttcga tgtgccgttg aagaaaaccg gggcttatca gtcgattgat attcgcttta
     5341 gttatgatat caacgggttg cttgaagttg acgtgcttct ggaagacggc agcgttaagt
     5401 ccagagtgat taaccacagc ccggtaacat tgagcgcgca gcagattgaa gagagtcgga
     5461 cgcggttatc cgcattgaaa atttatccgc gcgatatgct catcaatcgc acctttaaag
     5521 ccaaactgga agagttgtgg gcgcgggcgc tgggtgacga gcgagaagag attggccggg
     5581 tgatcaccga ttttgatgcg gcgctgcagt caaacgatat ggcccgcgtc gatgaggtca
     5641 ggcggcgggc gagcgtttat ttagccattg agacctcata atcacggctg ccggatgcgg
     5701 cgtaaatgct gcatccggca tcggcgcaaa aaacacatcg tcaactaaac aacatcgcga
     5761 ttttgccgag agcataacct ataaccagca ccgttaaact gattttgata atgctggtaa
     5821 tgctctgtgg tttttcaccc tcatcatttt tttcttcatg gttttcaggt gttggctcat
     5881 cgacatcttc atcctccggc tcgagtttta ctttcgtcaa cagcatccgg gtgataatgg
     5941 cctctaatgg atgagcatct ccgccttcca gccttgtatt aagacgggcc agcatctctt
     6001 gtttcggaaa cgcttcaagt actacctgtt ccgctacccg atacaaatcc agtaacggtg
     6061 aatcttcttc tgtctcgcta ccgactctcg atttccacca cgcccatgct ttgcgtttga
     6121 agaataacga ctcaataaac gattgaccta acggcgataa ttttccgcta tgtaaaattt
     6181 ccgcccataa cagccgcgtc cgggcgctct caccaggaac atatttcagc ggttgacctt
     6241 gcgcatctac catgctccgc gcttcgataa ccatcactaa taacgcgaaa taatccgggc
     6301 aatgacgctt gctccactgc aacaacaaat tatcagcttg tgtagatggg taactttccc
     6361 agtacgcgcc gagatcagca agcaggcttt ccccctcgcc gcagtacaaa cgttgtgcac
     6421 actggtaata ccgggcatct tcgctttctg gttcttccgc ttgccactgt ttggctacag
     6481 gaatgagttc caccatacct gtttgcaccg agctgtacca gcgcaacagt ttgcgatgta
     6541 actttgcatc atcaggaatg aaccacggtc cttccatcgc caaaaacgca gtgacatagt
     6601 caggatggta acggcagcaa cgttccagcg cgaagtaata ttcaacagtc tggttctgta
     6661 acgcgacggg atattccaga aggctaagga aatcgaaaca atcaccagcc tgaatggcct
     6721 caagaaaagc ctccctttct tcctcatcca ttccttcgct gtcattaaac gaatgccagt
     6781 tcaaacgctc tgccaacaaa gaagcacaat ttaatgacag atatcgcgct tctatagcga
     6841 ttgcacacag cggccagcgt aattgatcga catcctccat gttccaggta tttaattgct
     6901 gaataaattt ctgccaggcg gagggctgaa aacgatcact ttctgaatcc agtaatgtcc
     6961 tgaatgcacg tagtatttca tgttcagcgg tggcatcttt ttcttcatca tctgcttcct
     7021 cgacaggatt taccgccagt cgcagcgcct cttcataggc ctggcgtaat gctttaaacc
     7081 cctgcggatc ggtttcggga tgacacaacg gtaagcgagc aagataagcc tggcggataa
     7141 tgtctatttg cgtcgtgctt tcaatttcga ggatttgcca acatgttttc ataggtcgct
     7201 cacatcctta agcgttaccg ggcattcagg ctggacagtt aatggctgcc agggaagcgt
     7261 ggcatagaaa cggtcgcttc ccgctgccag ctcttcgaac atccgactcc cggcgctatc
     7321 gtatttgtac agggttagcg cctcgcgtag cgaagcgttg tcttcgcact gagaagattg
     7381 ccagtagaga cgccctagtg aataggcaga aaaatagtgc atccagctgt gatagtaatg
     7441 gtgagctcgt gcatagacgc gcgattgcag ccataaactc tcttcttccg tcagccagtt
     7501 gttgagcaca ccggcccgtg acagaaatcc cattcgaaca taatcccagg ctttgattcc
     7561 cccttctcca cagcacatag cggtttgggc aacgaattgg gtataaatca accctctctc
     7621 cgagccacca gcaattaatg ctttccactc ttcaggggaa gagcgttgcc atgtcagata
     7681 taagccagct aaatcattgg catgaccatc atcagtcatc tgataaatag tctgaattaa
     7741 atcatcacga ttatttattc cccaggatga tgagagatcg aattctttta tcccggtata
     7801 catttttggc gagtcatgcg cgggtttgca actctgatta agtatttcca tgggcgcaga
     7861 aagcgcaaaa agaagatatt gttcttcctt attcattttt taataatccc ttaagtgcat
     7921 taactgacta cttttttatt attgatgagt ataaacttga cgctttcaaa ataaaaagaa
     7981 aatcgaagca ttcacacatg aataaaaaat taatgtatat attcgcaatt tttatagttg
     8041 cagcaattac ctgtattagc caacccaaga aaacgatgtt gcgtgataaa gccatggtga
     8101 attatgcctt tgattattta agatcaccag gcagtctacc gttaaccacg gcagccacgg
     8161 agctttccgc gattcatggt cactcaacat cgcaatatcg ccttggcgaa ttttatcttc
     8221 atggtagcga cggtaaacca ctggattata cacaggcgag atactggtat gagcaatcag
     8281 cagaacagga aaatccgcgc gcacaaagca aactggggtg gatctacctg aaaggcctgg
     8341 gagtcaaacc cgatacccgt aaagcaattc tctggtataa ggaagcagct gaacaagggt
     8401 atgctcatgc tcaatatact ttgggtttga tctacagaaa tggcactggt attaatgtta
     8461 accattatga atctcaaaaa tggttaaaac tggccgccaa acaacattac aaaaatgcgg
     8521 aaagattact tgccggactt cccgcacatt aacgtcgact gtcacccgaa gagatggagt
     8581 attttcccta tcaaaccggc aaagataaaa atgtagaaaa taattttgat gacgcccaga
     8641 cagcccagcc cgccatcatc aacgggcatt ggctgcggtt gcggctccgg ctcgacggtt
     8701 tcctgaccgg taaaattcgc cgcctgaatt aataatcgac gaataattaa tgcttccagt
     8761 ggacaacgat cttctgcatc aaggcggctc acaatcggca ttaatgattc ttctgtcggt
     8821 tggctggcct gcactaattg tttggcaact aaatataatg gccataaggg atgttctgta
     8881 tcacgcgtga gtgagatccg cggcttacgt cctttgtgca aaaccatctc gaccagtgaa
     8941 cgccccagcg cagagagttt ttcatcgctg agtatttcgt ataaatgaaa acgcgtacgg
     9001 gcgctgtcgc cggggacata aagtagcggt tttccctgat cgttaaccag atcacgcgct
     9061 tcaatcatca tcaccactaa tgggtaataa tagacccgat gctgacggca ccattgcaac
     9121 attaaagcat ccgcctgggt ggagggatac tcgcgccagt agtaacaaag ttctgccagc
     9181 aggctttcgc cttctccaca atagacgcgt tgtgcgtatt cataataacg cgcatcttca
     9241 ttatcaggtt cagcttcccg ccacgaccgg gcgtaatcca ggagttcggg gattcccagg
     9301 cggcttgcgc tataccagcg gagtaaattt ctgtgcagtt ttgcatcatc ggggataatc
     9361 actgcgcgat gttgcgctaa atacaatgtc agccattctg gatgcgatga ccaaatccga
     9421 gcgaacattt ggtaaaaatc gatggtgtca ttttgcacgg caactggcag atgcagaatg
     9481 gtctggaagt taaaaacatt ccccttcgca atggcatata aaaaggattc cagttcttct
     9541 tcgtctattt ccccgacgtt ttcctcctgc aaccaccgca atctttctgc taataacacc
     9601 acgcactcga aggataaatg ggcagtgttc atggcgattg tgcacagcga ccagcgtaat
     9661 tcatcaatct cttccatcga gcaataattt aattgctgaa tgaatcgctg ccaggcggag
     9721 ggcagaaaac gttcgctatc agaggcaagt aacgcgcgaa aggcgagcag aatttcatgt
     9781 tctgctacct catattcttc tggttgccaa acagatttag caggcgactg cgcaatccgt
     9841 agcgcctcct catacgcctg acgaagttgt ttaaaaccct gcggatcggt ttctggatga
     9901 aaggacggta acagcgccag ataagcgcgg cggataatat cgacgtcagt cgtttcctct
     9961 atatcgagga tcttccagca atttttcata agtcgctcat atccttaagc gtttccgggc
    10021 atgtggggta gtcagccagt ggttgccagg acaaggtagg gtaatacgca tcatcactgg
    10081 caaataattg ataaaacata tcattgccgg aatcgtcgta ttctttacgc gctaagaatt
    10141 cgcgtaataa aggcagatga tcatcttcgg gagactgcca atattgccga ccaaaggtat
    10201 aaccggcaaa gtattgccgc cagttactat aataacgtag agcccgtaga tgaatacgcg
    10261 actggatcca tagactttct tcttcactca accagttatt aagtacgccc attcgactta
    10321 aaaaacccat ccggacataa tcccatgctt taattcctcc ctcaccacag cattcggctg
    10381 tgctggcgac aaattgcgca taagcctgac cttgctcatt taattcagca agatagtcac
    10441 gccattcaca tggagaataa cgaaaccagc ggtgataaaa ccctgccaac cgagcggcat
    10501 gaccgttctc gatcattcgg tgaattatgc gcagcaggtc atcacggtca tcaattcccc
    10561 atgaggaaga aaggtcaaaa aagcatgtac ctaaatacat tttgggggat gaatgcgaag
    10621 gtaaacagca ttcattgcgt atttccatcg gagtggaaag tgcaaacaat attttttgcg
    10681 accccatgtc catataattt atccttaaac attcatttaa tgaatgtaaa ctacatttcg
    10741 aggctcagtt atttatactt ttctgacgca acataatacg cataaaataa ggcttacgca
    10801 aaaatggaac acataaaaat ataatatatt ttatctacaa ccataaggaa atgataatga
    10861 tttttacgtc aagttgctgc gataatttat caatagatga gattatcgaa cgtgctgaaa
    10921 aaggcgattg cgaggctcaa tatattgttg ggttttatta taatcgcgat agcgcaattg
    10981 actctccgga cgacgaaaag gccttttact ggctgaagct ggccgctgag caaggtcatt
    11041 gtgaagcaca gtattcctta gggcaaaagt ataccgagga taaaagccgt cataaagata
    11101 atgagcaagc catcttctgg ctgaaaaaag ctgccctaca aggccatact ttcgcttcca
    11161 acgcccttgg ctggatactg gatcgtggag aagaccccaa ctataaagaa gcggttacct
    11221 ggtatcagat agccgcggag agcggaatgt cttatgcgca aaataatctt ggatggatgt
    11281 acagaaatgg caacggagtc gcacaagact atacgctggc atttttctgg tacaaacaag
    11341 ctgcattaca aggccatagt tacgcacaag acaatctggc cgatctttat aaagacggag
    11401 aaggcgttgc tcaaaacaag acactcgccg cattctggta tttgaaaagc gcacagcagg
    11461 gtaatcggca cgcccagttt caaattgcat gggattataa cgctggcgaa ggggtggacc
    11521 aggactataa gcaagcgatg tactggtatc tgaaggctgc cgctcaggaa agcgtcggcg
    11581 cttacgtcaa catcggttat atgtataaac acggacaagg cgttgagaaa gattatcagg
    11641 ctgcctttga atggtttacg aaagccgctg aatgcaatga cgccactgcc tggtataacc
    11701 tggccattat gtatcactac ggagaaggaa gacctgtcga tctccgacag gctctcgacc
    11761 tgtatcgtaa agttcagtca tccggaacca gagatgtcag tcaggaaatt cgtgagactg
    11821 aagctttact gtagaaataa aaacaagggc cggggaattt cattctgccc atattctctt
    11881 tattgctgtg cgaaatcaga ctattacggc tcaaacgggc gtctctggaa ctggtcataa
    11941 ccacatttcg ggcatagcgt cagcacttcc ggtgtgtaga tcgggagatg gaagtgacat
    12001 ttctcgcaga ccagatttcc cagcccgacc acttctccgc tgtgataaac cccatgatga
    12061 ttgaggtcct ggaaaatttc gcgccattca agctgcgttt tatcggtgat gtctgccagc
    12121 tcctgccaca agctttcttt aatcacccgc ataaagacgc tgtcagattc ttctttcagg
    12181 ctctcttcat agctcatggc gaactcttcc aggtcacgtc tgacagctcg cgtcagctca
    12241 tcgatctcgg ttcgcgttaa ctcccctgtt tttattacgc gctcgcgcgc ctgttccacc
    12301 agtgcgtcga tatcacgttc gccattgcgc aggcgttcgt tcagtgacgc gaccagttca
    12361 cggtaatatt gagcaacctt gttcatcgtt tcatctcccg ggtcgttaac tgactataat
    12421 aatagacgtt ctttgttcct tgctttgcta atacgcccac aaactctgta aattaatgta
    12481 cgaataatcc gggcatcatt tcaacgtcga gtgcgcgaaa ggctgttttg acgcggacgt
    12541 ttgggctatg ctatgcggat ctgaaaaacc acctcaacgc tacatttgta gccgtattga
    12601 aaacaggacc actggctgcc atgcaagagc aataccgccc ggaagagata gaatccaaag
    12661 tacagcttca ttgggatgag aagcgcacat ttgaagtaac cgaagacgag agcaaagaga
    12721 agtattactg cctgtctatg cttccctatc cttctggtcg actacacatg ggccacgtac
    12781 gtaactacac catcggtgac gtgatcgccc gttaccagcg catgctgggc aaaaacgttc
    12841 tgcagccgat cggctgggac gcgtttggtc tgcctgcgga aggcgcggcg gtgaaaaaca
    12901 acaccgctcc ggcaccgtgg acgtacgaca acatcgcgta tatgaaaaac cagctcaaaa
    12961 tgctgggctt tggttatgac tggagccgcg agctggcaac ctgtacgccg gaatactacc
    13021 gttgggaaca gaaattcttc accgagctgt ataaaaaagg cctggtatat aaaaagactt
    13081 ctgcggtcaa ctggtgcccg aacgaccaga ccgtactggc gaacgaacaa gttatcgacg
    13141 gctgctgctg gcgctgcgat accaaagttg aacgtaaaga gatcccgcag tggtttatca
    13201 aaatcactgc ttacgctgac gagctgctca acgatctgga taaactggat cactggccag
    13261 acaccgttaa aaccatgcag cgtaactgga tcggtcgttc cgaaggcgtg gagatcacct
    13321 tcaacgttaa cgactatgac aacacgctga ccgtttacac tacccgcccg gacaccttta
    13381 tgggttgtac ctacctggcg gtagctgcgg gtcatccgct ggcgcagaaa gcggcggaaa
    13441 ataatcctga actggcggcc tttattgacg aatgccgtaa caccaaagtt gccgaagctg
    13501 aaatggcgac gatggagaaa aaaggcgtcg atactggctt taaagctgtt cacccattaa
    13561 cgggcgaaga aattcccgtt tgggcagcaa acttcgtatt gatggagtac ggcacgggcg
    13621 cagttatggc ggttccgggt cacgaccagc gcgactacga gtttgcctct aaatacggcc
    13681 tgaacatcaa accggttatc ctggcggctg acggctctga gccagatctt tctcagcaag
    13741 ccctgactga aaaaggcgtg ctgttcaact ctggcgagtt caacggtctt gaccatgaag
    13801 cggccttcaa cgccatcgcc gataaactga ctgcgatggg cgttggcgag cgtaaagtga
    13861 actaccgcct gcgcgactgg ggtgtttctc gccagcgtta ctggggcgcg ccgattccga
    13921 tggtgacgct ggaagacggt accgtaatgc caaccccgga cgaccagctg ccggtgatcc
    13981 tgccggaaga tgtggtgatg gacggcatta ccagcccgat taaagcagat ccggagtggg
    14041 caaaaactac cgttaacggt atgccagcgc tgcgtgaaac cgacactttc gacaccttta
    14101 tggagtcctc ctggtactat gcgcgctaca cttgcccaga gtacaaagaa ggtatgctgg
    14161 attccgaagc ggctaactac tggctgccgg tggatatcta cattggtggt atcgaacacg
    14221 ccattatgca cctgctctac ttccgcttct tccacaaact gatgcgtgat gcaggcatgg
    14281 tgaactctga cgaaccagcg aaacagttgc tgtgtcaggg tatggtgctg gcagatgcct
    14341 tctactatgt tggcgaaaac ggcgaacgta actgggtttc cccggttgat gctatcgttg
    14401 aacgtgacga gaaaggccgt atcgtgaaag cgaaagatgc ggcaggccat gaactggttt
    14461 ataccggcat gagcaaaatg tccaagtcga agaacaacgg tatcgacccg caggtgatgg
    14521 ttgaacgtta cggcgcggac accgttcgtc tgtttatgat gtttgcttct ccggctgata
    14581 tgactctcga atggcaggaa tccggtgtgg aaggggctaa ccgcttcctg aaacgtgtct
    14641 ggaaactggt gtacgagcac acagcaaaag gtgatgttgc ggcactgaac gttgatgcgc
    14701 tgactgaaga tcagaaagcg ctgcgtcgcg atgtgcataa aactatcgct aaagtgaccg
    14761 atgatatcgg ccgtcgtcag accttcaaca ccgcaattgc ggcgattatg gagctgatga
    14821 acaaactggc gaaagcaccg accgatggcg agcaggatcg cgctctgatg caggaagcac
    14881 tgcaggccgt tgtccgtatg ctcaacccgt tcaccccgca catctgcttc acgctgtggc
    14941 aggaactcaa aggcgaaggc gatatcgaca acgcgccgtg gccggttgct gacgaaaaag
    15001 cgatggtgga agactccacg ctggtcgtgg tgcaggttaa cggtaaagtc cgtgccaaaa
    15061 tcaccgtccc ggtggacgca acggaagaac aggttcgcga acgtgctggc caggaacatc
    15121 tggtagcaaa atatcttgat ggcgttactg tacgtaaagt gatttacgta ccaggtaaac
    15181 tcctcaatct ggtcgttggc taagcgcggg aggaagcgtg cgatatctgg caacactgtt
    15241 gttatctctg gcggtgttaa tcaccgccgg gtgtggctgg catctgcgtg acaccacgca
    15301 ggttccttcc actatgaagg tcatgatcct ggactcaggc gatccgaacg ggccattaag
    15361 ccgtgcggtg cgtaaccagt tacgtctgaa tggtgtcgag ttgcttgata aagaaaccac
    15421 gcgtaaggac gttccatccc tgcgtttggg taaagtgagc atcgcgaaag ataccgcatc
    15481 ggtattccgt aacggtcaaa cagcagagta tcagatgatc atgacggtga atgcgaccgt
    15541 gttgatcccc ggccgtgata tctacccgat tagcgccaaa gtcttccgtt cattcttcga
    15601 taacccgcaa atggcgttag cgaaagataa cgaacaagac atgatcgtaa aagagatgta
    15661 cgaccgtgct gccgaacagc tgattcgtaa gctgccaagc atccgtgctg cggatattcg
    15721 ttccgacgaa gaacagacgt cgaccacaac ggatactccg gcaacgcctg cacgcgtctc
    15781 caccatgctg ggtaactgat gattcggttg tacccggaac aactccgcgc gcagctcaat
    15841 gaagggctgc gcgcggcgta tcttttactt ggtaacgatc ctctgttatt gcaggaaagc
    15901 caggacgctg ttcgccaggt agctgcggca caaggattcg aagaacacca cactttttcc
    15961 attgatccca acactgactg gaatgcgatc ttttcgttat gccaggctat gagtctgttt
    16021 gccagtcgac aaacgctatt gctgttgtta ccagaaaacg gaccgaatgc ggcgatcaat
    16081 gaacaacttc tcacactcac cggacttctg catgacgacc tgctattgat cgtccgcggt
    16141 aataaattaa gcaaagcgca agaaaatgcc gcctggttta ctgctcttgc gaatcgcagc
    16201 gtgcaggtga cctgtcagac accggagcag gctcagcttc cccgctgggt tgctgtgcgc
    16261 gcaaaacagc tcaacttaga actggatgac gcggcgaatc aggtgctctg ctactgttat
    16321 gaaggtaacc tgctggcgct ggctcaggca ctggagcgtt tatcgctgct ctggccagac
    16381 ggcaaactga cattaccgcg cgttgaacag gcggtgaatg atgccgcgca tttcacccct
    16441 tttcattggg ttgatgcttt gttgatggga aaaagtaagc gcgcgttgca tattcttcag
    16501 caactgcgtc tggaaggcag cgagccggtt attttgttgc gcacattaca acgtgaactg
    16561 ttgttactgg ttaacctgaa acgccagtct gcccatacgc cactgcgtgc gttgtttgat
    16621 aagcatcggg tatggcagaa ccgccggggc atgatgggcg aggcgttaaa tcgcttaagt
    16681 cagccgcagt tacgtcaggc cgtgcaactc ctgacacgaa cggaactcac cctcaaacaa
    16741 gattacggtc agtcagtgtg ggcagagctg gaagggttat ctcttctgtt gtgccataaa
    16801 cccctggcgg acgtatttat cgacggttga tatgaaatct ttacaggctc tgtttggcgg
    16861 cacctttgat ccggtgcact atggtcatct aaaacccgtg gaaacgctgg cgaatttgat
    16921 tggtctgacg cgggtcacaa tcatccctaa taatgttcct ccgcatcgtc cccagccgga
    16981 agcgaacagc gtgcagcgta aacacatgct tgaactggcg attgccgaca agccattatt
    17041 tactcttgat gaacgcgagc taaagcgcaa tgccccctct tacactgcgc aaacactgaa
    17101 agagtggcgg caggaacaag gaccggacgt gccgctggcg tttattattg gtcaggattc
    17161 attgctgacc tttccgacct ggtacgaata cgaaacgata ctcgacaatg cacatttgat
    17221 cgtctgtcgg cgtccaggtt acccacttga aatggcgcaa ccgcaatacc agcaatggct
    17281 ggaagatcat ttgacacata acccggaaga tcttcacctt cagcctgccg gtaaaattta
    17341 tctggctgaa acgccgtggt ttaacatctc ggcgaccatc atccgcgaac gtttgcaaaa
    17401 cggtgaatcg tgtgaggatt tattgccgga accggtattg acttacatta accaacaagg
    17461 cttgtatcgc tgatacctgt cgttacggag ggcatgatgc gactgtggtt aattcgtcat
    17521 ggcgaaacac aagcgaatgt cgatggtctg tacagcggtc atgcgcccac acccctgacc
    17581 gcacgcggta ttgagcaagc gcaaaatctg catacactgc tagatgatgt ttcctttgac
    17641 ttggttttat gcagtgaact ggaacgggca cagcataccg cgcgactggt tctcagtgac
    17701 cgccagctcc ccgttcatat catccctgaa ctcaacgaaa tgttttttgg cgactgggag
    17761 atgcgacatc atcgcgacct catgcaagaa gatgccgaaa actatagcgc gtggtgcaat
    17821 gactggcagc atgctatccc cacgaacggt gaaggatttc aggcattttc gcaacgtgtg
    17881 gaacgcttta tcgcaaggct tagtgaatat cagcactatc agaatatttt aatcgtcagc
    17941 catcagggcg tactgagtct gttaattgct cgtttaattg gcatgcctgc cgagtctatg
    18001 tggcattttc gcgttgacca ggggtgttgg agcgccattg atatcaacca aaaattcgcg
    18061 acgctacgcg tcctcaatag ccgtgccatc ggggtcgaga acgcatgact tttctgtttt
    18121 tttacgggta agccgcaacg accattgaca gccccgggca ggctgatatt ctccgcagcc
    18181 agactttttc cgccagacac gactttgtag aaattgtttt acaaaaatgg cgatgcaatc
    18241 tgcggcgcgg ggtgggatga tagcccactt tcgaaagccg attcggcgac aattgtcccg
    18301 aaatcgcctc tggttcaggt atactgacag accattttta tctatttgat tcacccaggg
    18361 ggaaaacttg cagggtaaag cactccagga ttttgttatc gacaaaattg atgacctcaa
    18421 aggtcaggac atcatcgcct tagacgttca aggcaaatcc agcatcaccg actgcatgat
    18481 catctgtacg ggtacgtcca gccgtcatgt tatgtccatt gctgaccacg ttgtgcagga
    18541 gtctcgcgca gcgggcctgt taccgctcgg cgtagaaggt gaaaacagcg ccgactggat
    18601 tgtcgtggat ttgggcgatg tgattgtcca tgtcatgcag gaagagagcc gtcgcctgta
    18661 tgaactggaa aaactctgga gttaatgcgt gaagctgcaa cttgtcgccg tgggaacgaa
    18721 aatgccggac tgggtacaaa ccggttttac cgagtacctg cgtcgttttc cgaaagatat
    18781 gcccttcgag ctgattgaaa ttccggccgg caaacgcggc aagaatgcgg acatcaagcg
    18841 catactcgac aaagagggtg agcagatgtt ggcggccgca ggcaaaaacc gcattgtcac
    18901 cctcgatatt ccaggcaagc cctgggatac gccgcagtta gccgctgagc tggaacgctg
    18961 gaagctggat ggtcgcgacg taagtctact gattggcggg cctgaagggt tgtcgcctgc
    19021 ctgtaaagcg gcggctgagc agagctggtc gctgtcggcg cttaccctcc cccatccgct
    19081 ggttcgcgtg ctggtcgcag agagtctgta ccgggcgtgg agcatcacca ccaaccatcc
    19141 ttatcaccgt gagtgataag ggagctttga gtagaaaacg cagcggatga aactacagaa
    19201 ctcttttcgc gactatacgg cagagtccgc gctgtttgtg cgccgggcgc tggtcgcctt
    19261 tttggggatt ttgctgctga ccggcgtgct tatcgccaac ctgtataatc tgcaaattgt
    19321 tcgctttacc gactaccaga cccgctctaa tgaaaaccgc attaagctgg tgcctatcgc
    19381 gcccagccgc ggcattatct acgaccgtaa cggtatccct ctggccctca accgcactat
    19441 ctaccagata gaaatgatgc cggagaaagt cgataacgtg cagcaaacgc tggacgcttt
    19501 gcgcagcgtg gtagatctga ccgatgacga tattgctgca ttccgaaaag agcgcgcacg
    19561 ttcacaccgt ttcacctcta ttccggtgaa aactaaccta accgaagtac aagtagctcg
    19621 ctttgccgtc aatcagtacc gttttccggg tgtcgaagtt aaaggctata aacgtcgtta
    19681 ctatccttac ggttcggcgt tgacccacgt catcggctat gtgtcgaaaa tcaacgataa
    19741 agacgtcgaa cgcctgaata atgacggcaa actggccaac tatgcggcaa cgcatgatat
    19801 tggtaagctg ggcattgagc gttactatga agacgtgctg cacggtcaga ccggttatga
    19861 agaggttgaa gttaacaacc gtgggcgtgt tatccgccag ttaaaagaag taccaccgca
    19921 agccggacac gatatttacc tgacgctgga tctcaaactc cagcaatata ttgaaacgct
    19981 gctggcgggt agccgcgcag ctgtggttgt taccgaaccg cgtacaggtg gggtgctggc
    20041 gctggtttcc acgcctagtt atgacccaaa cctgtttgtt gacggtatct ccagcaaaga
    20101 ttattccgcc ttgctgaacg acccgaatac accgctggtg aaccgcgcca cacagggggt
    20161 ttatcctccc gcgtctacag ttaaacccta tgtggcggtt tcggcattaa gcgccggggt
    20221 gatcacgcgc aatacgacgc tgtttgaccc aggctggtgg caactaccag ggtcggaaaa
    20281 acgttatcgt gactggaaaa aatggggcca cgggcgtctg aatgtcacaa gatcgctgga
    20341 agaatctgcg gataccttct tctatcaggt ggcctacgat atggggatcg atcgcctctc
    20401 cgaatggatg ggtaaattcg gttatggtca ttacaccggt atcgacctgg cggaagaacg
    20461 ttccggcaac atgcctaccc gcgaatggaa acagaaacgc tttaaaaaac cgtggtatca
    20521 gggtgacacc attccggttg gtatcggcca gggttactgg acagcgaccc caatccagat
    20581 gagtaaggca ctgatgatcc tgattaatga cggtatcgtg aaggttccgc atttgctgat
    20641 gagcaccgcc gaagacggca aacaggtgcc atgggtacaa ccgcatgaac cgcccgtcgg
    20701 cgatattcat tccggttact gggagctggc gaaagacggt atgtacggtg ttgctaaccg
    20761 ccctaatggt acggcgaata aatactttgc cagcgcaccg tacaaaattg cggcgaaatc
    20821 tggtaccgct caggtcttcg gtctgaaagc gaacgaaacc tataatgcgc acaaaattgc
    20881 cgagcgttta cgtgaccaca aactgatgac cgcctttgcg ccatacaaca atccgcaagt
    20941 ggctgtcgcc atgattctgg agaacggcgg cgcgggtccg gcggttggta cgctgatgcg
    21001 ccagatcctc gaccacatta tgctgggtga taacaacacc gatctgcctg cggaaaatcc
    21061 agcggttgcc gcagcggagg accattaatc atgacggata atccgaataa aaaaacattc
    21121 tgggataaag tccatctcga tcccacaatg ctgctgattt tactggcatt gctggtttac
    21181 agcgccctgg ttatctggag cgccagcggt caggatattg gcatgatgga gcgtaaaatc
    21241 ggccaaatcg cgatgggtct ggtcatcatg gtggtgatgg cgcaaattcc tccacgcgtt
    21301 tatgaaggct gggcccccta tctctatatc atctgtatta ttttgctggt ggcggtagat
    21361 gctttcggag ccatctctaa aggcgctcaa cgctggctgg acctcggtat tgttcgtttt
    21421 cagccgtcgg aaattgccaa aatagccgta ccactgatgg ttgcgcgctt tatcaaccgc
    21481 gacgtttgcc cgccatcgtt gaagaatact ggcatcgcgc tggtgctgat atttatgccc
    21541 acgctgctgg tggctgcaca gcctgacctg ggaacatcaa tcctcgttgc gctttccggt
    21601 ctgtttgtac tgttcctctc tggccttagc tggcgtctga ttggcgtcgc agtagtgctg
    21661 gtagcggcgt tcattccgat tctgtggttc ttcctgatgc atgattacca gcgccagcgc
    21721 gtaatgatgc tcctggaccc ggaatcagac ccactcggcg cgggctatca cattattcag
    21781 tctaaaattg ctattggctc cggcggatta cgcggcaaag gctggctgca cggcactcag
    21841 tcacagcttg aatttctccc cgaacgccat actgacttta tcttcgcggt actggcggaa
    21901 gagctgggat tagtgggcat tctgattctg ctcgctctct acattctgct gatcatgcgc
    21961 gggctgtgga tagccgccag agcgcaaacc acctttggtc gcgtcatggc tggtggctta
    22021 atgctgatat tattcgttta tgtcttcgta aatattggta tggtaagcgg tattctgccg
    22081 gttgtagggg ttccgctccc actggtcagt tatggaggat cggcgctaat tgtgctgatg
    22141 gctgggttcg ggattgtaat gtcaatccac acccacagga aaatgttgtc gaaaagcgtg
    22201 taagaggtgc gcaatgcgta agcagtggct cgggatctgc atcgcggcag gaatgctcgc
    22261 ggcatgtaca agcgatgatg gtcagcaaca gacggtaagt gtaccgcagc ctgcggtatg
    22321 taacggcccc atagttgaaa ttagcggggc ggacccgcgt ttcgaaccac tgaacgcgac
    22381 ggcaaatcag gattaccagc gcgacggtaa aagctacaaa atcgtgcagg atccgtctcg
    22441 atttagccag gcgggactgg cggcaatcta tgatgccgaa ccaggcagta acctgacggc
    22501 ctctggcgaa gctttcgatc cgacaaagct gacggcggcc catccaacgc ttccgatccc
    22561 cagctacgcc agaatcacta acctggctaa cgggcgaatg atcgtggtgc gcattaatga
    22621 tcgcggccct tacggcaacg accgcgttat ttcgctttct cgcgcggcag ctgaccgtct
    22681 taacacgtca aacaacacca aagttcgtat cgatccgatt attgtcgccc aggatggttc
    22741 gctttctggt cctggtatgg cttgtaccac agtcgccaaa cagacttacg ccctgcctgc
    22801 acctcccgat ttaagcggtg gcgcgggaac aagttcagtg tctggcccgc agggtgacat
    22861 tcttccggtc agtaattcga cgctaaaaag cgaagatccg accggcgcgc cggtaaccag
    22921 tagcggtttc ctcggcgctc caacgacctt agcgcctggt gtactggaag gcagcgaacc
    22981 gacgcctgcg ccacagcccg ttgttacagc ttcgtcgaca acgcctgcaa cctcgcctgc
    23041 aatggtgaca ccgcaagccg cctcgcaaag cgccagcggc aactttatgg tgcaagtcgg
    23101 ggccgtaagc gatcaggctc gtgcgcaaca gtaccaacag caactgggac agaagttcgg
    23161 cgtccccggt cgcgtaactc aaaatggcgc ggtctggcgg atccagcttg gcccattcgc
    23221 cagcaaagcc gaagccagta ccttgcagca acgtttgcaa accgaagccc aattacagtc
    23281 atttattact accgcgcagt agcgtaaagc aggcatctga tgtgtcaatt tctgtaaatg
    23341 acgttaacaa agtcacgcgg aaagtcagat gcctgccggt agtgcatttg ctatagtagg
    23401 gcactttttt aattccatca cggatgtcgt agttcagacc atgaatacca ttttttccgc
    23461 tcgtatcatg aagcgcctgg cgctcaccac ggctctttgc acagccttta tctctgctgc
    23521 acatgccgat gacctgaata tcaaaactat gatcccgggt gtaccgcaga tcgatgcgga
    23581 gtcctacatc ctgattgact ataactccgg caaagtgctc gccgaacaga acgcagatgt
    23641 ccgccgcgat cctgccagcc tgaccaaaat gatgaccagt tacgttatcg gccaggcaat
    23701 gaaagccggt aaatttaaag aaactgattt agtcactatc ggcaacgacg catgggccac
    23761 cggtaacccg gtgtttaaag gttcttcgct gatgttcctc aaaccgggca tgcaggttcc
    23821 ggtttctcag ctgatccgcg gtattaacct gcaatcgggt aacgatgctt gtgtcgccat
    23881 ggccgatttt gccgctggta gccaggacgc ttttgttggc ttgatgaaca gctacgttaa
    23941 cgcactgggc ctgaaaaata cccacttcca gacggtacat ggtctggatg ctgatggtca
    24001 gtacagctcc gcgcgagata tggcgctgat cggccaggca ttgatccgtg acgtaccgaa
    24061 tgaatactcg atctataaag aaaaagaatt tacgtttaac ggtattcgcc agctgaaccg
    24121 taacggcctg ttatgggata acagcctgaa tgtcgacggc atcaaaaccg gacacactga
    24181 caaagcaggt tacaatcttg ttgcttctgc gactgaaggc cagatgcgct tgatttctgc
    24241 ggtgatgggt ggacgtactt ttaaaggccg tgaagccgaa agtaaaaaac tgctaacctg
    24301 gggcttccgt ttcttcgaaa ccgttaaccc actgaaagta ggtaaagagt tcgcctctga
    24361 accggtttgg tttagtgatt ctgatcgcgc ttcgttaggg gttgataaag acgtgtacct
    24421 gaccattccg cgtggtcgca tgaaagatct gaaagccagc tatgtgctga acagcagtga
    24481 attgcatgcg ccgctgcaaa agaatcaggt cgtcggaact atcaacttcc agcttgatgg
    24541 caaaacgatc gagcaacgcc cgctggttgt gttgcaagaa atcccggaag gtaacttctt
    24601 cggcaaaatc attgattaca ttaaattaat gttccatcac tggtttggtt aaaaattgaa
    24661 cacttgaaag tgtaatttcc gtccccatat actaagcatc agtaaaaaaa ctcccgcctt
    24721 ctggcgggag ttgctattta attacgttac gccggagctg acatgaaaac caaacttaac
    24781 gaactgcttg aattccctac tccttttact tacaaagtta tggggcaggc gttacctgag
    24841 ctggttgatc aggtggttga agtggtacag cgccatgcgc caggtgacta caccccaacg
    24901 gtaaaaccaa gcagcaaagg caactaccac tcggtatcta tcactatcaa cgccactcat
    24961 atcgagcagg ttgaaacact gtatgaagaa ctgggcaaaa tcgatattgt ccgcatggtt
    25021 ctgtaactcg cttctccgtt acccggctcc tggtcgggta actcccctct cctcgctgtg
    25081 atatactttt cccccacttt tactcattct ccacggagat gccgttttgt atcaggataa
    25141 aattcttgtc cgccagctcg gtcttcagcc ttacgagcca atctcccggg ctatgcatga
    25201 attcaccgat acccgcgata atagtaccct tgatgaaatc tggctggtcg agcactatcc
    25261 ggtattcacc caaggtcagg caggaaaagc ggagcacatt ttaatgccgg gtgatattcc
    25321 ggtgatccag agcgatcgcg gtgggcaggt gacttatcac gggccgggac aacaggtgat
    25381 gtatgtgttg cttaacctga aacgccgtaa actcggtgtg cgtgaactgg tgaccttgct
    25441 tgagcaaaca gtggtgaata ccctggctga actgggtata gaagcgcatc ctcgggctga
    25501 cgcgcctggt gtctatgtcg gggaaaagaa aatttgctca ctgggtttac gaattcgacg
    25561 cggttgttca ttccacggtc tggcattaaa cgtcaatatg gatctttcac catttttacg
    25621 tattaatcct tgtgggtatg ccggagtgga aatggctaaa atatcacaat ggaaacccga
    25681 agcgacgact aataatattg ctccacgttt actggaaaat attttagcgc tactaaacaa
    25741 tccggacttc gaatatatta ccgcttaatt ccatacatca atggcccaat ttacactggg
    25801 tcattactca taatacctgc taatgcttta catctccttc gcgtttgtat attttcttta
    25861 tcacttgcag aaatgcggtg ccacgccttg ctccgtaact caccccattg attaacctat
    25921 tgaaatggtt gattatggtg agcaaaagcg ttccgttcca attagacata agattattgt
    25981 aattttttaa atattatttt tccatgaata aaattggagt aagtgcgtgg atagtaataa
    26041 tcaaattgaa ccctgtttaa gcagaaagtc gtcagaaggt aaaccacaaa tatttacaac
    26101 cttacgaaat attgatctta accttctgac tatttttgaa gctgtatatg tacataaagg
    26161 gatcgttaat gcagcgaaag tgcttaatct gaccccctcg gcaatcagtc agtctattca
    26221 gaaactgcgc gttatattcc ctgacccatt gtttattcgc aaaggccagg gtgtcactcc
    26281 taccgcattt gcgatgcatc tacatgagta tatcagtcag ggccttgagt ccattcttgg
    26341 cgcgctggat atcgaaggaa gctatgataa gcaacgaacg ataactattg cgaccactcc
    26401 ctcggtcgga gccctggtcc ttcctgtcat ctatcgggcg attaaaactc actatccgca
    26461 gcttttactg cgcaacccgc ccatcagcga cgcggaaaac caactcagtc agtttcaaac
    26521 cgatctcatc atcgataaca tgttttgcac caatcgtacg gtgcaacatc atgttctgtt
    26581 caccgacaat atggtgttaa tttgccgcaa gggaaatcca ctactctctt tagaagatga
    26641 cagagagact atcgacaacg ctgcgcatgt acttctgtta ccggaagggc aaaatttcag
    26701 cggtctgcgg cagagagttc aagagatgtt tccggaccgg caaatcaatt tcaccagcta
    26761 caacattttg acaatcgctg cactggttgc caacagtgac atgttagcga ttattcccag
    26821 ccgtttttat aacctgttta gtcgttgctg gccgctggaa aaactgcctt ttccgtcctt
    26881 aaatgaggag caaatagatt tctctatcca ctacaacaaa ttcatcctgc gtgatcccat
    26941 cctgcacgga gtaatagatg ttatccgtaa tgcattttga aaaagtattg ttacagccag
    27001 atcacagcgc agaacgataa aatggcacaa tccaacaaca attttacatt ttcgcgaccg
    27061 ctttggctgc tttcaggtcc gtttcaatga tatactgcca gtcgttaatt caaaaatagt
    27121 tgataattac aacaatctat tgaattgaaa cgctttcctt cgtaattcgc aactggaaca
    27181 cgcacgctat gagtaaaccc attgtgatgg aacgcggtgt taaataccgc gatgccgata
    27241 agatggccct tatcccggtt aaaaacgtgg caacagagcg cgaagccctg ctgcgcaagc
    27301 cggaatggat gaaaatcaag cttccagcgg actctacacg tatccagggc atcaaagccg
    27361 caatgcgcaa aaatggcctg cattctgtct gcgaggaagc ctcctgccct aacctggcgg
    27421 aatgcttcaa ccacggcaca gcaacgttta tgatcctcgg cgctatttgt acccgccgtt
    27481 gtccgttctg tgatgttgcc cacggtcgcc cggtagctcc tgatgccaat gaaccagtga
    27541 aactggcgca gaccattgcc gatatggcgc tgcgttatgt ggttatcacc tccgttgacc
    27601 gtgatgacct gcgcgatggc ggtgcccaac actttgcgga ttgtattact gccattcggg
    27661 aaaaaagccc gcaaatcaaa attgaaactc tggtgccgga tttccgcggt cgaatggatc
    27721 gtgctctgga tattctgact gcaacgcccc cagatgtgtt caaccataac ctggaaaacg
    27781 taccgcgtat ttaccggcag gtacggcctg gcgcagatta caactggtcg ctgaagctgc
    27841 tggaacgctt taaagaagcg catccggaaa tcccgaccaa gtctggtctg atggtgggtc
    27901 tgggtgaaac caatgaagaa attattgagg taatgcgcga cctgcgccgt catggtgtga
    27961 cgatgttaac gctggggcaa tatttgcagc caagccgcca tcacctgccg gttcaacgtt
    28021 acgttagccc ggatgagttc gacgaaatga aagccgaagc gctggcgatg ggctttaccc
    28081 atgctgcatg cggtccgttt gtccgctctt cttaccacgc cgatttgcag gcgaaaggaa
    28141 tggaagttaa gtaatcctgt aataattact tacatctgtc tgataaaaaa cccgcttttt
    28201 gaagcgggtt ttttgtatcg aacaagatat tgagggagcg tcctgctcgc cacgtcactc
    28261 tttatgagag agcttttcag cctgaagatc aacgtctgcg ccttttttcg ccgcagcatc
    28321 gtcatcattc atcgccttct tgaacccttt aatggccgct ccaaggtctc cgcccagcgt
    28381 acgtaacttc ttagtcccaa acagcagaac gaccagcgcc gcaactacca gcagtttggt
    28441 aatactaatc tcacccatag ataccttctt gacatataac cggcaacaga acgcgcctta
    28501 aaaaccagag aatattaacg gtcattcgac gcgcgcacac aatagcaatc tgtaacaagg
    28561 tgaatcaagc gccgagtaaa aaaacatcat aataattgcg gcggcgcaaa gcgacggttg
    28621 tttaagatgg gcagttgcgc gcgcacctga cgcacacgtt cgggcgtcac ttccgccata
    28681 atgagtgcag gcatttctga cgctgccgca atggtgacgc caaagggatc tataatccgg
    28741 ctttgcccga tatttttgtt cccgcactcc cccgccgcca ccatataaca ggtggtatcc
    28801 agcgcacgag cggcaagcaa cgttgaccag tgatgctctt tgagcggccc acgaacccag
    28861 gcggcaggaa gtaccaggat ttcagctccc tgtaaagcct gtgccagcgc cagctctgga
    28921 aagcgtaagt cataacaggt catcagaccg accttcatcc cttccacctc cagtaacgga
    28981 gctatttcat taccagcatc aacatggcgt gattcctgaa tggcaaatgc atcatagaga
    29041 tgcagtttgg cataacgggc gacgatgttt cctgcctgaa gtgccaccag catattccat
    29101 gcgcgccccg gcgttgaagg aagatgaatc gtcagaattg tcgtcatcat gttacgttta
    29161 ctttctcgcc gtaaacgtcc gaggaattcg ccttccagca gctgtgctga tttaaccgat
    29221 agatctgcat catgatcatc gcgcgccagc aatgcttccg gcaggacaaa cagcgatacg
    29281 tcgttttccg ccgcctgcgc catcaacgag gcacaaatct cagcgttctt ttcccacaca
    29341 gatgtaacag caaactgtcc tgcagcaacc agcattgcca cctcctgtca gagtcgtaaa
    29401 attcgccaac gacgattatt cggcgttaca cttatcactc atacaaatca aatagcagga
    29461 ttttgcagtg ttacaacttc ttttagcagt ttttattggc ggtggtacgg gaagcgtagc
    29521 gagatggctg ttaagtatgc gatttaaccc gctgcatcag gcgattccgt tggggacgct
    29581 ggcagcaaac ctgattgggg cattcatcat aggaatggga ttcgcctggt tcagcaggat
    29641 gacgaacatt gaaccagtgt ggaaagtatt aatcaccacc ggattttgtg gcggtctaac
    29701 aaccttctca acattttcgg cagaagtggt gtttttgtta caagagggcc gctttggctg
    29761 ggcattactg aacgttttcg tcaaccttct ggggtctttt gccatgaccg cactggcatt
    29821 ctggctgttt tcggcctcaa ccgtacacta aagaccaaaa aaaacccgct gattaagcgg
    29881 gttttgaatt cttgctgacg tatcttacag agcgattacg tttgcagcag aagggccttt
    29941 ggcaccgtta gtgatttcga actctacgcg ctgaccttca gcaagagttt taaaaccatt
    30001 agtctggatt gcagagaagt gtacgaacac gtctttgctg ccgtcttccg gagtaatgaa
    30061 accgaatcct ttggactcat taaaccactt aacgttacct ttaatcttag acatcaaaat
    30121 tacctttaca tgaaaaatag acacaaatgc tgtgtcggtt atcagtacac caattgtggt
    30181 acgctttgtc cagtgcaact ttactaaaaa gtgataaaag tcgcgttatt tttgatgttg
    30241 cttattttag ttattgattt taaagaagtt actaaaactt catttgtctc aaaactgaaa
    30301 gcgcatccag gcaaagtaca cattgccatt gttgtaggta cccggaatgt aggtcatctg
    30361 aaaagtcact gggccataac ccacggaggc caatggcagt agaaccggga gagggatgta
    30421 attccagtta tcgcgtgccg ttacgccagc ggtgaatccc agacctaaat gaaaattttc
    30481 atccgccagc ggtcgccagg tactttccca tccgtatccg gcaatcggtt cccatttgtt
    30541 ccacgagtcc ttaaatgcca tggcatacag gccatgccag tttccttttt catcccaacg
    30601 cgacaggcca aaaccgccac cccacggtcg ctcgttatag cgatcggttt tttctttgtc
    30661 gtaagcgaaa cgtgcatgcc aggtgatggc aggaatatat aaatcataat gttcaggctg
    30721 ttgccaggtt tgtgcaatat tttctctaaa cgttgtcatc ctctcatctg cgttagcaaa
    30781 aactttacca acgctgatta actgaataaa aacaaaggaa aagatagcga catatttact
    30841 cacgttcatt tgtgaccata aaacatttat caaaaatcta ctactagcat agcaaagcta
    30901 caattaacat aaccttaata gacccaacat aaagaataat ctgaatagct gctctattta
    30961 tttccgcttg ttcaagttaa atatttccgc gcatcgatta aagatgatgc gtcgttttct
    31021 gcgatgggaa tagtcaaaaa agaaaaacca agtctttttt gatgacaaaa tgcaatcaag
    31081 gaaaaattat tttatttttt taacgagata caacaatcat cttaacgaag tatataatat
    31141 atatcgtcaa cccatgctca tattagatta ttttttgagc gcacagccgc agctaacacc
    31201 attgcaatta acaaatttgc atcaatccac catcaattta cacacattat tatgtgataa
    31261 ttgccaaccg ctaaatatgc gttttgttat ctatgtataa aaacagcaac ttcaatgtct
    31321 taatggcagt ttttcttgat tttaatcagc attcatcgcc aatttattgg gcatattttt
    31381 tccttaagct ttaggaagtt tttattattt actttgggcc tggagacagg aaaaattatg
    31441 ctgacattca ttgaactcct tattggggtt gtggttattg tgggtgtggc tcgctacatc
    31501 attaaagggt attccgccac tggtgtgtta tttgtcggtg gcctgttatt gctgattatc
    31561 agtgccatta tggggcacaa agtgttaccg tccagccagg cttcaacagg ctacagcgcc
    31621 acggatatcg ttgaatacgt taaaatatta ctaatgagcc gcggcggcga cctcggcatg
    31681 atgattatga tgctgtgtgg ctttgccgct tacatgaccc atatcggcgc gaatgatatg
    31741 gtggtcaagc tggcgtcaaa accattgcag tatattaact ccccttacct gctgatgatt
    31801 gccgcctatt ttgttgcttg tctgatgtca ctggccgtct cttccgcaac cggtctgggg
    31861 gttttgctga tggcaaccct gtttccggtg atggtaaacg ttggtatcag tcgtggcgca
    31921 gcagctgcca tttgtgcctc cccggcggcg attattctcg caccgacttc aggggatgtg
    31981 gtgctggcgg cgcaggcttc cgaaatgtcg ctgattgact tcgccttcaa aacgacgctg
    32041 cctatctcaa ttgctgcaat tatcggcatg gcgatcgccc acttcttctg gcaacgttat
    32101 ctggataaaa aagagcacat ctctcatgaa atgttagatg tcagtgaaat caccaccact
    32161 gcccccgcgt tttatgccat tttgccgttc acgccgatca tcggtgtact gatttttgac
    32221 ggtaaatggg gtccgcaatt acacatcatc actattctgg tgatttgtat gctgattgcc
    32281 tccattctgg agttcctccg cagctttaat acccagaaag ttttctctgg tctggaagtg
    32341 gcttatcgcg ggatggcaga tgcgtttgct aacgtggtga tgctgctggt tgccgctggg
    32401 gtattcgctc aggggcttag caccatcggc tttattcaaa gtctgatttc tatcgctacc
    32461 tcgtttggtt cggcgagtat catcctgatg ctggtattgg tgattctgac catgctggcg
    32521 gcagtcacga ccggttcagg caatgcgccg ttttatgcgt ttgttgagat gatcccgaaa
    32581 ctggcgcact cttccggcat taacccggcg tatttgacta tcccgatgct gcaggcgtca
    32641 aacctgggtc gtaccctatc acccgtttct ggcgtagtcg ttgcggttgc cgggatggcg
    32701 aaaatctcac catttgaagt cgtaaaacgc acctcggtgc cggtgcttgt tgggctagtg
    32761 attgttatcg ttgctacaga gctgatggtg ccaggaacgg cagcagcggt cacaggcaag
    32821 taaatagtaa tgccggaggc aagtttcctc cggctttatc atgtcaccca ctgtggtata
    32881 tgcgttgtgg tctgccaact ttgccgtgaa caatttcagc aataatcaga tggcggctgg
    32941 cgcaatattc aagataacgc ctggcagtgg tgcggctgat ggtcagtgcc tgcgccaccg
    33001 tttctgccgt atgttgcaca ccaggctctt taaacagttt tcgcaccgcg tttagcgtca
    33061 aggggtcaat gccggtcggc agttcgtctt tgggttcacc gcgagcatag gcattaaaca
    33121 tctcatcaat ttgcttctgg ctggcgctat caatactttc cagcatatgt ttacgctggc
    33181 ggaaacgggt tagcgtttgc cccagtgtat tgattcactt gaagtacgaa aaaaccggga
    33241 gaacattgga ttattcggga tctgatggga ttagatttgg tggggcttgc aagcctgtag
    33301 tacaaatttt aggcgttaac caatgaaacg cgaaagatag taaaaaattg cttttgtttc
    33361 attgaaaaaa cgaaaaacaa aaacactata aatcatttca atcatagctt caaaaaacgt
    33421 tcaaaaccga taacaaccag gctgtcatca aatgactcat atcacaaatc agctcatgcc
    33481 gtttaggtat gttacatgtg tgattatgtg aggtgaagta tgttttagct ggctcatggt
    33541 tgttatacgg cttttttcgc atcttgtggt tccaacaaag gagctacagg ctcttcctgt
    33601 ttatgaacac catacattga caaaatctct tggcgagttt ctggaggtaa tttatgcagc
    33661 gccaaagctg tttgtagtgt ttggcgatca atacccagcg ttgccaatag ggtgtcaacg
    33721 gagatggagg ggcgttcttg cattaatccg gaaagcccat atttagccat gtttagctct
    33781 atctctcgga cttcattttc tcctagttta ttgattaaca gggagaacat tttcaatgta
    33841 tcagcattca tttcttcgat agcaattttt tgcatagccg gtattgccgg gatatattca
    33901 accccaggcc cgcgacgccc ttcttgtgaa cgcttcggcc atccttcttt ctttgctcgt
    33961 ttatgcaccc cctgtattgt cccaggcatt ccatccagtt cagtaatttg tttgggtgtc
    34021 atccactggt ttatctcatt tgcgcttttc ataaactaag tttaccagat ttatctatat
    34081 aaaaatcaat ggattacaaa ataaaccaca ttaactgata taaaccggtt tggtttattt
    34141 ggtaatgtat aaaacagata atcaagacga attatttgtt ttggtaaccc tttatcatac
    34201 gcaatatggt tggtgtgcga aagatgagta acgattggca tcgggccgac gtgattgcgg
    34261 ccctcaaaaa aacaggaacc cctctggcaa aattgtccag acgttcaggt tattcaaccg
    34321 ggacgcttgc aaatgcatta gtcaggccat ggcctaaagg tgaacgcatt attgctgacg
    34381 ccttaaacat gaaacctgag gaaatttggc ctagtagata tctaagggat cacaatgaga
    34441 gagtggatat cagcaattga atgcatgggt ttatctggtt tacctggttc attaatgggg
    34501 gttcacaaaa gagctcagag agaaggatgg ctcaagagaa gaagagaagg aattagaggg
    34561 gttgcttacg aataccatat atcttctctt cctaacattg ctcgagctga agttttgctc
    34621 caacaaggaa aatttaaaac cagtcaggga tgttttgaaa tcgcccgccc cacgctggaa
    34681 gctcatgatt atgatcgtga ggcactatgg agcaaatggg ataaagccag cgattcccag
    34741 cgcagcctgg ctgaaaaatg gttgccctca attcaggcca cagacgaaat gctgaaccag
    34801 gggatttcaa cgaaaacggc ttttgcgacc gttgcagggc attaccaggt cagcgcatcc
    34861 actttgcggg acaagtatta ccaggtacag aagtttgcga agcctgactg ggcggctgca
    34921 cttgttgatg gacgtggagc atcccgtcgc aatgttcaca aaagtgaatt tgacgaggat
    34981 gcctggcagt ttctgattgc agattatctg cgaccggaaa aacccgcctt ccgcaaatgt
    35041 tatgagcgtc tggaactggc agcccgcgag catggctgga gtattccctc ccgtgccacg
    35101 gcctttcgcc ggattcagca actggacgag gcaatggttg ttgcctgtcg tgaaggtgaa
    35161 catgcactga tgcatctgat accggcacag cagcgaactg tggaacacct ggacgccatg
    35221 cagtggatca acggcgacgg ttatctgcat aacgtctttg tacgctggtt taacggtgat
    35281 gtgatccgtc cgaaaacatg gttctggcag gatgtgaaaa cccgaaaaat tctgggctgg
    35341 cgctgcgatg tgagcgagaa cattgattca attcgcctct cgttcatgga tgttgtgact
    35401 cgctacagta tcccggagga ttttcacatc accattgata acacccgtgg tgctgcgaat
    35461 aaatggctga cgggaggcgc gcccaatcgc taccgcttta aggtaaaaga ggacgatcca
    35521 aaaggactgt ttttactgat gggtgcgaaa atgcactgga caagcgttgt tgccggtaaa
    35581 ggctggggcc aggcaaaacc tgttgaacgt gctttcggtg ttggtgggct tgaggaatac
    35641 gttgataagc atccggcact ggctggcgca tatacggggc caaatccgca ggcaaaacct
    35701 gataactatg gcgaccacgc tgttgatgca gagctgtttc tgaaaacctt tgccgaaggt
    35761 gtggcgatgt tcaatgccag aacaggccgt gaaacagaaa tgtgcggggg caaactctcg
    35821 tttgatgatg ttttcgagcg tgaatacgcc agaacgattg tgcgtaagcc aaccgaagaa
    35881 caaaaacgga tgctgttact gcctgccgag gcggtgaacg tttcacgcaa aggcgagttt
    35941 acgcttaaag ttggcggctc ccttaaaggc gcgaaaaacg tttattacaa catggcatta
    36001 atgaatgccg gagtgaaaaa agttgtggtc agatttgatc cacagcagtt acacagcacg
    36061 gtttattgag
//
LOCUS       DAJTSI010000021        36049 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000021 DAJTSI010000000
VERSION     DAJTSI010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.021"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OO327_000982"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OO327_000982"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR6017613.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OO327_000983"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OO327_000983"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR6017614.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OO327_000984"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OO327_000984"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR6017615.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OO327_000985"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OO327_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR6017616.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OO327_000986"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OO327_000986"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR6017617.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OO327_000987"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OO327_000987"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6017618.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="OO327_000988"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="OO327_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR6017619.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OO327_000989"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OO327_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6017620.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OO327_000990"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OO327_000990"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR6017621.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OO327_000991"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OO327_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR6017622.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OO327_000992"
     CDS             complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OO327_000992"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR6017623.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEVGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="OO327_000993"
     CDS             complement(15665..16702)
                     /locus_tag="OO327_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6017624.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17035..17523
                     /gene="yhhY"
                     /locus_tag="OO327_000994"
     CDS             17035..17523
                     /gene="yhhY"
                     /locus_tag="OO327_000994"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6017625.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="OO327_000995"
     CDS             complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="OO327_000995"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCR6017626.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19444..19884
                     /locus_tag="OO327_000996"
     CDS             19444..19884
                     /locus_tag="OO327_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR6017627.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNSDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="OO327_000997"
     CDS             complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="OO327_000997"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6017628.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20611..21601)
                     /locus_tag="OO327_000998"
                     /pseudo
     CDS             complement(20611..21601)
                     /locus_tag="OO327_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="OO327_000999"
     CDS             complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="OO327_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR6017629.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="OO327_001000"
     CDS             complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="OO327_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR6017630.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="OO327_001001"
     CDS             complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="OO327_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCR6017631.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25143..25856)
                     /gene="livF"
                     /locus_tag="OO327_001002"
     CDS             complement(25143..25856)
                     /gene="livF"
                     /locus_tag="OO327_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR6017632.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25858..26625)
                     /gene="livG"
                     /locus_tag="OO327_001003"
     CDS             complement(25858..26625)
                     /gene="livG"
                     /locus_tag="OO327_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR6017633.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26622..27899)
                     /gene="livM"
                     /locus_tag="OO327_001004"
     CDS             complement(26622..27899)
                     /gene="livM"
                     /locus_tag="OO327_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR6017634.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27896..28822)
                     /gene="livH"
                     /locus_tag="OO327_001005"
     CDS             complement(27896..28822)
                     /gene="livH"
                     /locus_tag="OO327_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR6017635.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28870..29979)
                     /gene="livK"
                     /locus_tag="OO327_001006"
     CDS             complement(28870..29979)
                     /gene="livK"
                     /locus_tag="OO327_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR6017636.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30403..30786
                     /gene="panM"
                     /locus_tag="OO327_001007"
     CDS             30403..30786
                     /gene="panM"
                     /locus_tag="OO327_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR6017637.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="OO327_001008"
     CDS             complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="OO327_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR6017638.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="OO327_001009"
     CDS             complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="OO327_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR6017639.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="OO327_001010"
     CDS             complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="OO327_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR6017640.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="OO327_001011"
     CDS             complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="OO327_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR6017641.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35180..>36049)
                     /gene="ftsY"
                     /locus_tag="OO327_001012"
     CDS             complement(35180..>36049)
                     /gene="ftsY"
                     /locus_tag="OO327_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR6017642.1"
                     /translation="NLGSGFISLFRGKKIDDDLFEELEEQLLIADVGVETTRKIITNL
                     TEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTI
                     GKLARQFEQQGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAI
                     QAAKARNIDVLIADTAGRLQNKSHLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQN
                     AVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFK
                     ADDFIEALFARED"
BASE COUNT         8659 a        10042 c         9046 g         8302 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatga cggcttaaaa tgtcctgaat ggtcgaggaa accaagaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcaca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tacctggctg attaatgccg aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctctaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagctgcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcagc cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tgttacgggt aactgttatc ggcaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctgaat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctacttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tggcacaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattgc cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgatacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    16981 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    17041 gagatagtaa tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc
    17101 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    17161 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    17221 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    17281 ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag
    17341 atgattgaaa tgtgcgacaa ctggttacgg gtagatcgca ttgaactaac cgtgtttgtc
    17401 gataacgcgc cagcaattaa ggtctataaa aaattcggct ttgagattga agggaccggt
    17461 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaaa
    17521 tagagaattt gcaatctaag tttgtcattt aagccctcac ctaccgggag ggcttaagat
    17581 attaataccc cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac
    17641 cgtccggccc aaccataatg ctttgtgtgc tgcccatcgc ctctttcagc gccactttct
    17701 gaccttttgc ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct
    17761 cgtccggcaa ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc
    17821 cataatcgat gctattcacc accatttgca gcaccgtggt gataatccgg ctaccgcctg
    17881 ggctaccggt aaccagccag gttttaccgt ctttcaccac aatggtcggc gacatcgacg
    17941 acagcgggcg tttgttcggc ccgacggcgt tggcatcacc gcccaccagc ccgtaaacgt
    18001 tcggtacgcc cggtttggcg gagaaatcat ccatctggtt attaagcaga ataccgctct
    18061 cacccgcgac aatacccgta ccgaaggtgg tgttcagcgt ataggtcacc gccaccgcgt
    18121 taccgtcttt atccactaca gagtaatggg tagtttgatt actctcataa ggcgcaagct
    18181 tgccagggcg aatctcactg gacggcttcg cttttttgat atcaatttga tcggcaatgg
    18241 atttggcata ggctttattg gtcagtgcct gccacgggac tttgacaaaa tccgggtcgc
    18301 caagatattc cgagcggtcg gcgtaggcgt atttctccgc ttctgccatg atttgcatcg
    18361 catcggcgct gccaaagccg tatttcttca tatcgaagtt ttccagaata ttgaggattt
    18421 gtacgatatg gatcccgccg gaggatggcg gtggcatgga gtaaacctga tacccgcgat
    18481 aatcgccgct tatcggagtg cgttcgaccg ctttataggc cgctaaatct tctttagtga
    18541 tcaagccacc gtttttctgc atctcctggg cgatctgttc cgcaatcgtg cctttataga
    18601 attcgtccgg gccgttttca gcaatcatct ccaggctctt tgccaggttc gcctgcacca
    18661 gcgtgtcgcc ctttttcagc ggctcgccct ctttccagaa gatagcttta ctgttttcgt
    18721 gattcggcaa cacttcgcta ccgtaggttt tgagatcgtc agccagcgcg tcgttaacga
    18781 taaaaccatc gcgtgccagt ttaaacgcgg gctgcacgac tttgttcagc ggcatggtgc
    18841 cgtatttatc cagcgccagc gagaaacctg ctaccgtacc cggtgtgccg gaagccagat
    18901 gcgaagtgag tgattttttg ctgtccgggt tgccctgatc atcgaggaac atatcgcggg
    18961 tcgctttggc gggtgccatt tcgcggaaat cgatagccgt ggtattgcca tttttcgagc
    19021 ggattaacat aaaaccacca ccgcccagat tccctgcctg cggatgcgtt accgccagcg
    19081 cgtagcccac cgccacggcg gcatcaacgg cattcccgcc ctccttgagg atatccaccc
    19141 ccacctgagt ggcagtggcg tccacagacg ctaccattcc ctgtttcgcg cgtaccggat
    19201 ggaagacatc ttcctccaca ccatacgaga cgggcggcgc aggaggcgcg gcggcggcgc
    19261 taaaacaact tcctgagagc agagcagcaa tggccacccg gcgtaaaaac gtcggtttta
    19321 tcatcgttat tctccagaga ttaaggggca accccagcta agcctggtat ataactctga
    19381 attaatcatc gttttgtcgg gaagcgagta aacttaaagg atatcttcag aggaggatac
    19441 gcgatgaaac gacttctgat tcttacggca ctcctgccgt ttgtcggctt tgcacagccc
    19501 attaatactc tgaacaaccc taaccagccg gggtatcaga tacccagcca gcagcggatg
    19561 caaacccaga tgcagactca gcaaatccag caaaaaggga tgctgaatca gcaactgaaa
    19621 acgcaaactc agttgcaaca gcagcattta gaaaaccaga taaacaataa ttctcagcgg
    19681 gtgttgcagt cacagccggg ggagcgaaat cccgcccggc agcaaatgct gcccaacacc
    19741 aacggcggga tgttaaacag caaccgtaat ccggatagtt cgttgaatca gcagcatatg
    19801 ctgccggaga ggagaaacag cgacatgctg aatcagccca gcacgccgca gcctgacatt
    19861 ccgttgaaaa ctattgggcc gtaaagttcg gaccaatcac gtcaatcgca tcggtacaga
    19921 tgcaatccac gccccagcgc agcaactctg ccgcgtgctg gggtttattg acggtataaa
    19981 ccagaatccg taatccggcg tctttcaact gcatcactcg cgctttatcg agtaacttat
    20041 gattgagatg aatagagacg cagcccagcc gcgcggtcag ttcgcgccag tcgtcgcgcc
    20101 actcatcaag caataaacca cgcggcagtt ccggtgccgc ctgttgtgca gcttctaaag
    20161 catcaatctc aaacgatgac agcagcggcg gcgtcatacc ggcccacagt tggcgtgccg
    20221 ccagcgccac cattttgccc gttaatggtc cagtgccagt ggtgggtttg atttcgatat
    20281 tcgccatcat cccgtgttcg cgacagcgtt ccgccacctg cgaaagcaac ggcagcggct
    20341 cacctttaaa cgctttgctg taccaactgc ccgcatccac gcgcagtaaa tcctgccagt
    20401 tcagttcacc cgcgacgccc cagccgttgc tggtgcgttc gagattgtcg tcatggagca
    20461 ggaagatctc gccatctttc gataacttcg cgtcaaattc gatcatctta tgaccgtatt
    20521 ttgccccgac gtcgattgcc gccagggtgt tttccggggc cagcttaccg ccgccacgat
    20581 gagcgacgat gcggggataa ggccagttac tcatactcgt tgtcctgttt caccatcaaa
    20641 aagatgcagc tgattttccg ccagatgcag ccacagcgtg ctgcctgccg tcgggcgctc
    20701 ctgatgcgcc agtcgcacca ccagcttctg cttgcgagct tagcgcaata tgttccgggc
    20761 gaatgccgag ggtcatttta cgcccggcat actgacggta gccaccgttt agcggtagca
    20821 cgataccgcc gtccagttcg aaatgcgtgc cttcattatt catgcggcct gtcagcaggt
    20881 tcatcgccgg gctgccgata aaactcgcca caaacaggct ggcgggcttt tcgtagactt
    20941 ccaccggcgt gccaatctgt tcggcaacgc cgccgttcat caccattact cgctgggcga
    21001 gcgtcatcgc ctcaacctga tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt
    21061 gcagctgttg cagttcaaga cgcatctgta cgcgcagctt ggcatcgagg ttagagagtg
    21121 gctcatcaaa caagaacacc gccggatcgc gcacaatcgc gcgtcccatc gccacacgct
    21181 ggcgctgacc gccggaaagc tcgcgcgggc gacgtttgag cagaccgtcc agctccagaa
    21241 tgcgcgccgc ttctttaacg cgctcggcta tttgctgctt gcccatgccg cgaattttca
    21301 gcccccacgc catgttttct tcgacgctca tatgcggata aagcgcgtaa ttctggaaca
    21361 ccatcgcaat cccgcgatct ttcggctcca tttcggtcac gcgctggtcg ttgatccaga
    21421 tatcgccttc ggtcaccctc tccagcccgg caaccatacg cagcagcgtc gatttcccgc
    21481 agccagacgg cccgaccatc acgataaatt cgccatccgc cacatcaagg gtcagcggtt
    21541 taatgaccta ggttttgcca tcccagcttt tggttactgc ctgtaatttc agtcctgcca
    21601 tcttatttct cactatcgac caggccgcgc acgaaggcac gctgcatcac taaaacaatc
    21661 actactggtg ggatcagggt taacaacatc gccgccatca ctgagttcca ttccgtggtg
    21721 ccttcgccag tggcgatcat ccctttgatc cctgccacgg tggtgccgag atccacgtcg
    21781 gtaataatca acaacggcca caaatactgg ttccagccgt aaataaaggt gatcacaaac
    21841 agcgccgcca gattagtttt ggagagcgga aaaacgatgt cgcaaaagaa gcgcattggt
    21901 gacgcaccgt cgatccgcgc cgcttccacc agttcatccg gcagcgtcat aaagaactgg
    21961 cgaaacagaa acgtggcggt cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg
    22021 tcgagcatct tcaggttggc gatgacttcc accgtcggga agatacgtac ttcaaccggc
    22081 agcatcaggg tgataaaaat catccagaag aagaggttac gtagcggaaa acgaaaccag
    22141 acaatggcaa atgccgagag catcgagaca gtaattttgc cgagcgtaat gctgaacgcc
    22201 atcacaaagc tgttaagcaa catccgccag aacggcgcgc tatttgtgcc taccccgttc
    22261 acccagatgt tgtggatgtt ttccagcaga tgcgtgccgg ggatgagcgt catcggcgcg
    22321 gcgtagacgg cctgtttatc cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg
    22381 atcaccgcga tcccgaggat cagcatggta tggctgaata tcgtcagcca cggacggttc
    22441 tcaatcattg gtaacgcacc ttgctttcaa catagcggaa ctgcaccacc gtcagcacga
    22501 tgacgaggaa catcagcacc atcgactgtg cggcagacga agccagatcc agtccggtaa
    22561 aaccttcgcg gtagatctta taaatcagcg tcgtggtggc ctgaaccggc ccgccggacg
    22621 tggcggcgtc gatcaccggg aaggtgtcga agaaggcata caccaggttc actaccagca
    22681 ggaagaaact caccggggcg ataagcggca gcgcaatctt aaagaagcgg cgaatcggcc
    22741 ctgcaccgtc gatggctgcg gcttcgatca acgaacgggg aatggattgc agcgcggcat
    22801 agaagaacag aaagttgtag ctgatttgct tccacaccga ggcaaacacc accagaaaca
    22861 tcgcctgacc gctattttgc gcgtggttcc agtcgtagcc gaactcagcg agaaaatggg
    22921 tgatcagccc gcgaccgggg ttaaacagga agatccacaa tacggcggca acggcgggag
    22981 ccacggcata cggcagcaac attaaggttt ggtagaaacg actgccgcgc acgatgtact
    23041 ccaccagcgc cgcaaagaac agcgatacca gcaaaccgct gacggtgaca aaggtgctga
    23101 attttatcgt cgtccagaag gagtcgagat aatagctgtc atgaaacagc gtgacgaagt
    23161 tatccagacc gacaaactgg ctggagaaac caaacggatc gacgctttgt agcgagtacc
    23221 acaacgcttc gcccgcaggc cagataaaaa agataacggt gatgatgagc tgcggcgcga
    23281 ccagcagata aggcagccag cgcgagcgga acaccggacg ggatgatgac attacggtta
    23341 attcctgaac tgtgccggat gcgcttcgct tatccggcct acacgcgatg ccaggtcaga
    23401 taagcgaaac gcatccggca ttacactgat taagacttcg tcgatttctc aaagcggcgc
    23461 agtaactggt tcccacgctc aactgcggta tccagcgcct gctgtggcgt cttcttaccg
    23521 gtccacacgc tctcaagctc ttcatccaca atcacgcgga tctgcggcat gttgcccaga
    23581 cgaagccctt tggtgaacgg caacggcggc ttgttcagca tctgacgcgt cgcggtatcc
    23641 gcacctgggt ttttctcgta aaagccctgc tcacgggtca ggtcatacgc cgctttggta
    23701 attggcagat aaccggtttt ctgatgccac tcagcagcgt tttctggctt cgcgaggaag
    23761 tcgagaaact tcgccacgcc ggtatacgtt tctttatctt tgccctgcat cacccacagg
    23821 ctggctccgc cgataatggc gttttgtggc gcatctttcg catcggcgtc gtaaggcatc
    23881 atacctacgc cgtagttaaa tttggcgtac tcgcgaatgt tggcaagaga accggaagag
    23941 gcggtggtca tcgcgcaatc accgttatag aacttctcgg tggattcatc cttacgaccg
    24001 acgtagctga agtcgccctt cttgttcatc tcctcgagca tggcgatgtg tttcacctgc
    24061 tccggcttgt tgaactccag caccgcgtcc gtgccgtcaa agccgttgtt tttgctggca
    24121 aacggcagac cgttccaggc gctaaagttt tccagttgga tccagccctg ccagccgctg
    24181 gcgtagccgc acttaatacc ggaggctttc agttttgcgg aatagtccgc cagatcctgc
    24241 caggttttcg gcggctgttc cgggtctaac cccgctttct tgaaggcgtc tttgttgtaa
    24301 tagagaacgg aagtcgagct gttgaacggc tgggagagta agtggccggt tttgctgtcg
    24361 gagtagtaac cggaaaccgt cggcacaaac tgcgactcat cgaactgaat ccctgcctct
    24421 ttaaacacgt catacaccgg tttaatggct ttcgacgcca tcatggtggc ggtgccaact
    24481 tcataaacct gcaaaatagc cggagcgttg ccggtacgaa atacggcaat ccccgcgctt
    24541 aaattctgtt cgtagttgcc tttataggtc ggtacaattt tgtaatccgg attttcggcg
    24601 ttaaaacgtt gggccagaga atccacctct ttacccagtt ccccttccat agaatgccag
    24661 aacggaatgg tcgtcactgc ctgtgcattc cccattaacg ccagtccgag cgccagtgct
    24721 gaagctgtat aatgtaacgg tttcatcgtt tatctctctt gttgtaccga atgcgcgaat
    24781 tcacgcgttt tatgctcgcg gggtaacatg acatgctcga attacagaaa aataactttt
    24841 ttgttacatt tgtaagatag taaggtgtca gaaagatgac aaggcggtga cggcgtgggt
    24901 gagggaaaat gggagatggg gcacggataa gcggaaaaat agaaggtctg aatcaaactc
    24961 tacagattac tcatcgtttc atgccggatg cggcgtaaac gccttatccg gcctacaaga
    25021 tcgtgcaaat tcaatatatt gcaactcacc cagtaggcct gataagcgca gcgcatcagg
    25081 caattttgca tttgtcacct gtctcaaagg agtcttttga ctccttatca atcaacgtgt
    25141 tattacccgc ctaaatacgc acttctcact gcttcgttcg ccagcagcgc gtcgccggta
    25201 tcggagagca ccacatggcc gttttccagc acgtagccgc gatccgccag ctttagcgcc
    25261 tggttggcgt tctgctcgac gaggaagata gtcatcccct gctcgcgcag ctgctcgatg
    25321 gtgtcgaaaa tttgctggat gataatcggc gcaagaccga gcgatggctc atcaagtaac
    25381 aacaaacgcg gattgctcat cagcgcgcga ccaatcgcca gcatctgctg ttcaccgccg
    25441 gacatggtgc ccgcccgctg aacacggcgc tcatgcagac gtggaaacag ttcatacacc
    25501 cactttatgc gctcctgaaa ctggtcacgt tcggcaaaga agccgcccat cgccaggttc
    25561 tcttccaccg tcatccgcga gaagacgcga cgcccttccg ggacaatcgc taccgcttcg
    25621 cgcatgattt tcgccgtctg ccagtcggta atgtctttat catcaaacac aattcgcccg
    25681 ctggtggcgc gcggatcgcc gcataacgtg ccgagcaagg tggttttccc cgccccgttc
    25741 gcgccaatga gagtgacaat ctcgccctga ttgatatgca ggctcacctc atgcagcgcc
    25801 tggattttgc cgtagtgggc gctgactttg tcaaaggaca acatgacttt ttccatctta
    25861 tgcctcacct aaataggcac ggatcacgtc cgggttatta cggatttgct ccggcgtacc
    25921 gtttgccagt ggcgtcccct gattgaccac ataaattcgg tctgaaatcc ccatcaccag
    25981 cttcatatcg tgttcaatca acaaaatagt ggtgttgtga tgattgcgca gttcggcaat
    26041 cagctcatcc agctctttgg tttcttttgg gttaagacct gccgccggtt cgtcgagcat
    26101 taaaatctcc ggctgcgtca ccatgcagcg ggcaatctcc agacggcgtt ggtcaccata
    26161 ggccaggtta ctcgcctgac ggttggcgtg ttccagcaaa ccaatgcgct caagccaggt
    26221 ggcggcgcgg tcgagcgctt cgctctgggc gcgacggaaa gaaggcgttt tcaacaggcc
    26281 agagaacagc ccggttttca gttgctgatg ctgcgccacc agcaggtttt caattaccgt
    26341 catttcacgg aacagacgca catgctggaa ggtgcgcacc acgcccatgc gagcaatttg
    26401 ctgccccggt aaaccttcaa gatgctgatc gcgcagtaaa atggtgccgc cggtgggttt
    26461 gtagaaaccg gtcaggcagt taaaaaccgt ggtttttccg gcaccgttag ggccgattaa
    26521 cgagacgatt tcctgcgggt acagttcaag attgacgttg ttcaccgcca gcaggccgcc
    26581 gaagcgcatc atcagaccgt taacagataa taatggctga ctcatgcctg ctctcctttc
    26641 gctgcgccgt ttttcagctt cagttgcggg cgcgtcatgg gcagcaaacc ctgcggacgc
    26701 cagatcatca tcagcaccat caaaccaccg agcattaaca tgctgtattc gttgaaatca
    26761 cgcatcaatt cgcgcgacac caccagcaga acagccgcca gaatcactgc aaattgcgag
    26821 cccataccgc cgagcaccac tatcgccagc acaaaggccg attcagcaaa ggtgaaggat
    26881 tccgggctga caaagccctg acgcgccgca aacagcgttc cggcaaaacc ggcaaatgcg
    26941 gcgctgatgg taaaagcagt cagcttgata cggcgcgggc ttaagcccag cgaacgacag
    27001 gcgatctcgt cttcacgcaa cgcttcccac gcgcggccca gcggcatccg cagcaggcgg
    27061 ttaatgacaa acaggcttaa caccaccagc agcaacgcca ccaggtagag gaagataacg
    27121 cgatcggacg gatcgtattt caggccaaag aaattactga acgtgtccca gccgccttca
    27181 cgagcggtac ggctgaactc gagtccgaag agtgtcggtt tcgggatctg actgattccg
    27241 ttcgggccgc cggtgatttc agtgttattg agcagcaata tgcgcacaat ttcgccgaaa
    27301 ccaagggtaa cgatcgccag atagtcaccg cgcagacgca gcaccggaaa accgagcagg
    27361 aaacccgccg ccgctgccat cagtccggca atcggcaaac aggtccagaa ccccagaccg
    27421 taatagtgat tgagcagcgc aaaagtgtaa gcgccgatgg cgtaaaaacc gccgtacccc
    27481 agcaccagca gaccagaaag accaacaacc acattcagcc cgagaccgag gataatgtag
    27541 atcatggtca gagtggcgat atccaccgtc ccacgtgaca ccataaacgg ccacgccacc
    27601 acaagcacca gcagcgccac gaggaacagc ttttgcttca ccgtggaccc atcaatcgcg
    27661 ggcagaataa acttcggccc ggaaacgctt ttcaaccctt tctggaaagc cggtcgcaaa
    27721 agctggaaga aaaagaccac cgccgtgccg ataaacatcc actgccaacg gatgtctgaa
    27781 gccgtgtcga ccaccagttt ggtgccatcc agctccagtt gtacgcccat aaagacgccc
    27841 gccaggacaa agaacatcgc ggcagagagc agtgccattg caatatgcat cggtttcata
    27901 ctttctctac ctccgggcga cccagaatac cggtcggcat caccagcagc accagaatca
    27961 gcagggcgaa tgagaccaca tctttatatt ccgtactcag ataggcagaa gagagcgcct
    28021 ccgcaatccc caggatcagc ccgccaatca tcgcccctgg aatgctgcca atcccaccga
    28081 gcaccgcagc ggtaaaggct ttcatcccgg ccataaagcc gatatagggg ttaatgacgc
    28141 agtagaactg accgagcagt acgccagcca ctgccgccat cgccgcgcca ataacaaagg
    28201 tcaaagcaat cacccggtcg gtgttgatgc caagcaggct cgccattttc agatcttccg
    28261 cgcaggcacg acaggcgcgc cccatacggg aatagcgaat gaaaatcgtc agcgccagca
    28321 tggcgaggaa ggtgacaatc cagatcaccg cctgcatggt ggtaatagag gcagagaagt
    28381 tttcgctatg gcccaccacc cactgaccgt taaacaggct cggcagcgcc acgtcgcgcg
    28441 aaccttcggt caggctgacg tagttttgca ggaagatgga cataccgatt gcggaaataa
    28501 gcgcaatcag gcgcttagag ttacgcaccg ggcggtaagc tacccgttcg atactccagc
    28561 cgtaggcgct ggcgatgacg attgcgccga cgaatcccgc cgccaccagc agccagccgg
    28621 tatcaatgcc catcatcatc agcgcggcga tgatcataaa tgagacgtag ctgccaatca
    28681 tataaacctc gccgtgggcg aagttgatca tgccgataat gccgtaaacc atggtgtagc
    28741 cgatggctat cagcgcgtag gtactgccca gcgtgacgcc gttaaacatc tgctgcaaga
    28801 aatacaaaaa ctgctcagac ataaggtaac ctttctaaac ccgcccgcat tttacgggcg
    28861 gtgggatgat cacttggctg ccgtggatga accgtcggcg tgccactgga agacaccaaa
    28921 atcaaatccc ttaagatcgc ctttttcatc ccagttcagc ggcccaatca cggtgtttgc
    28981 accgttagct tttaaatctt tcaccagcgc cagcggctca tcgctgccgg tacgctcaag
    29041 ggcagttgcc agagattgca ctgccgcgta agtgatccag acataaggcc cggacggatc
    29101 tttcttgtct gctttcagcg catcaacgat gccctggttt gccggatcct ggtcatagcg
    29161 ttttggcata gtgaccaaca tgccttcggc ggcatcaccg gcaatgttcg acagcgatgc
    29221 gttaccgaca ccttccggcc ccataaactg ggttttcagg ccaacggaac gggcttggcg
    29281 cagcatctgc cccatttccg ggtagtaacc gccgtagtaa acgaagtcga tattttcttt
    29341 tttcaggcgg gcgatcagcg cggagaaatc tttctccccg gcggtaatac cgtcgaagaa
    29401 gacgacgttg gcgttagccg ctttcagccc gtcctgcacc gaacgcgcca gcccttcgcc
    29461 atactgttgt ttgtcgtgaa tgatggcgat gcgctggggc ttcaccgtct caagaatgta
    29521 ttttgccgcc gtaggcccct gggaagagtc cagcccggca gtacgcataa tgtgttgata
    29581 accgcgttgg gtcagctccg ggttggtcgc tcccggcgag atcatcagaa taccttcgtc
    29641 ttcgtagata tccgacgctg gctgggtaga ggaagaacac agatgaccaa taacgtattt
    29701 aatgccgtcg ttaacgattt tgttggcgac cgcaacggct tgtttcgggt cgcaggcgtc
    29761 gtcatattcc acgccaacca gcttgtcgcc cttaattccc cctttgacat taatgtcttt
    29821 aatcgcctga cgcgcgccgt taaattccat atcgccccac tgggcaatcg ggccggacat
    29881 cgcgccgaca acggcgactt taatatcgtc agccatagcg gtgtgtgaaa ttgccagtgc
    29941 aatcatccct gcgatgatag ttttcgcatt ccgtttcata gtcaaaaatc cccattcgtg
    30001 atgttgtgtt gctttgtttt tatgtgttaa caaatcagac tgttcttttt ttatactgca
    30061 ctgtttttgc ctgtctgatt aaggggttag cgcagtattt tgtgatgata gcgattaaaa
    30121 tccctatttt tcagtcgatt aagaacagat aatattctga atttattgat agataaacag
    30181 aaaaaagtgc ctttgtcagc ataaaataac ggcacaaagg gcggaataat tcactatcat
    30241 tcaggggatt atgctggaca tttttcattc tctaatgttt taactttgta attattgctg
    30301 ttaaaaaatt aatcacctgc caaaagaaat aaaaaagaga aagcctccga ttaaattatt
    30361 tcgctacact ggttccactt ttgtgattta cacgggttac ccatgaagct gaccatcatt
    30421 cgattagaaa actttagcga ccaggaccgg attgacctgc aaaagatctg gccggagtat
    30481 tccccttcct ctttacaggt tgacgataac caccgtatct acgccgcgcg ttttaacgag
    30541 cgtctgcttg cggccgtgcg ggtaacctta agcggcaccg agggagcact ggattctcta
    30601 cgcgtgcggg aagtcacccg ccgtcgcggt gtggggcaat atctgctgga agaggttttg
    30661 cgtaacaatc ctggcgtttc atgctggtgg atggcggatg caggcgtgga agatcgcggt
    30721 gtgatgacgg cgtttatgca ggcactggga tttacggcac agcagggcgg ctgggagaag
    30781 cgttaatcgt caagttgatt tcaaaagtga tgaccaacga gaaattgcct gatgcgctac
    30841 gcttatcagg cctataatgt gtgctgcaat ttactgattt ctttggttct tgtaggccgg
    30901 ataaggcgtt tacgccgcat ccggcatgaa gcaacgtatt cgatattagc aatttggcgg
    30961 caacccaaag ttgccgatta acaattactt cgcatcggtc gccgtgccgt tggcgtgcca
    31021 gtcaaatacg ccgaactcaa agccctttag atcgcctttc tcatcccagg tcagtggccc
    31081 catcacggta tccacggagt tcgcttttag gtatttggcg atttcagccg gatcgtcaga
    31141 ctgattcagg cccgcctgca aagattgcag cgcggcgtag gtggtccaga cgaatgcgcc
    31201 gcttgggtcc tgttttttcg ctttgatcgc atcaacaatg ggtttgttcg ccggaacctg
    31261 atcgtagttc ttcggcttgg tcaccagcag cccttccgct gattcgcccg caatgttaga
    31321 cagcgaaacg ttagccacac cttccggccc cataaactga gttttcagcc ctgccgcgcg
    31381 tgcctgacgc aggatttgcc ccatttccgg gtgataaccg ccgtagtaaa cgaagtcgat
    31441 attctctttt ttcagacgcg ccaccagcgt tgagaaatct ttttccccgg cggtgatacc
    31501 atcaaagaac accacgttag cattgccttt cttcaggccg tcctgcaccg ctcgtgccag
    31561 accttcgccg tattgctgtt tgtcgtgaac gatagcaata cgctgcggtt tcactttctc
    31621 aagaatatat ttcgccgccg tcggcccctg gtcggagtcc aggccggtgg tgcgcaggat
    31681 cagctgatag ccacgggcgg tcagctccgg cgcggttgcc gctggggtga tcattaaaat
    31741 gccttcgtct tcgtagatgt cagacgcagg ctgcgttgat gaagaacaga ggtgaccaat
    31801 cacatattta atgccgtcgt taacgacttt gttcgccacc gcaaccgcct gtttcgggtc
    31861 acaggcatcg tcatatttta cgatttgcag tttgttgcct ttaatgccgc ctttagcgtt
    31921 gatatccgca accgcctgct ctgcgccggt aaactcctgg tcaccgtact gcgcaaccgg
    31981 accggacatt gcacccacga ccgcgacttt aatatcttct gccagagcca tattgctgaa
    32041 tgccagcgcg atacatcctg ccagtaacgc tttacccttt atgttcatcc tgagaatccc
    32101 cattcttctg gttattacgt gtgttgtgat gttgttgttc agcactttat ttcgttttat
    32161 gcatgactac ccgtgcttta gcagcatact ctgctaaaac atacccgatt tttatgatat
    32221 tggaatagct attttgacag tttattaaca atctgcgtgg ggattggcgt tttgccggag
    32281 ggggaattga ttattgtaga ggcccaaaaa acaaaaaccc cggactctca tccagggttc
    32341 tctgcttaat agcggaaatt acgcttcaat agcggcgcga agttttttca tcgcgttctt
    32401 ttccagttgg cgcacacgtt cagcggaaac accgtaacgg tcagccagtt cctgcaacgt
    32461 ggacttgttg tcttcgtcca gccagcgcgc acggatgatg tcctggctgc gttcgtccag
    32521 accatgcatc gcgtcggtca gacggtttgc cgcctgctct tcccagttat catcttcgat
    32581 gccgtcggca aagttagatg atttatcctg cagatagagc accggtgcca tcggctggct
    32641 gtcggaatcg tcgtcggaag acaggtcaaa ggtcatgtcc tgtgccgcca tacgtgattc
    32701 catctcacgt acgtctttgc tggttacgcc cagttcacgg gccaccattt cgacttcatc
    32761 ctggttaaac cagcccagac gctgcttggt tttacgcagg ttgaagaaca gtttgcgctg
    32821 cgctttggtg gtcgcaactt tgacgatacg ccagttacgc agaacgtatt cgtggatctc
    32881 tgctttgatc cagtggacgg cgaaggagac caggcgcaca cccacttccg ggttaaaacg
    32941 gcgcactgct ttcatcaggc cgatgttacc ttcctgaatc aaatccgcct gtggcaggcc
    33001 atagcccgca taattacgag caatatgaac aacaaaccgc aggtgagaca ggatcagcgt
    33061 tttagctgct tccagatcgc catggtaatg cagcttttca gccagcgccc gctcctcgtc
    33121 agccgacaac atcggccacg cgttagctgc ccggatgtag gaatccaggt tgccaactgg
    33181 ggctaaagct aaactttgca ttttgtcagt cattcaaatc ctctcaatcg atatcttctg
    33241 gcgcttcagt ggtagcaaca actgtgccaa agcttaagag caacgaggtt atcattcact
    33301 gttttatcag accgtgattt tatccacaag ttcaatgcaa gcttgtgaat aaattacgca
    33361 caaaatgtga catagagatg aaataccggg aagagacaac ggggtctctt tccctgctac
    33421 ggaacccatt gcagggaaag agtataacac gcttttatta ttcaggcgta aagtggcgta
    33481 aatgttgtac cgtggcaagc cacgctgcca cccagccaat catcgagcat accagcagca
    33541 atagcaggca ttcatcgaac gataaaccat tgatatcaaa cttcgttccg aaaacctgtg
    33601 ccacttccgc caccgctgac gacaaacgca gtaccagaat ttctgacaaa attaatgaca
    33661 acaatgcgcc agaaaatccc agcagcgcac cgccgtacag gaacgggcga aggatgaaac
    33721 catccgtcgc accaatcagt ttctgcacgt tgatggagtc acggcgagca aagatgctca
    33781 gacgtacgct gttgccgatg acgaggaaca ctgccgccac catcaacacg ccgatcatcg
    33841 ccgaaacgcg cccaaccagc ccggtcaacg ccgccagacg ggcaaaccag ctgtcatcca
    33901 tccgcacttc gtcaataccg ttaatctgcg tgatacgatc acgcagcgta ttgagtgatt
    33961 ccgtcccctg aaaatcgagt ttcgggatca ccaccgccac tgccggaagc gggttttctt
    34021 ccagcatatc cagcgcacca ccaaaaccag accagttacg gaactcgccc agtgcgtctt
    34081 cacgagaaag atagttcact ttctccacgc cttgctcggc ctgcaactgt gccaccacgc
    34141 ccgcagcggc gtcatcgtcc agcgtttttt gcaagtaaac agtgatttgc ggtgacggat
    34201 aatactgcgt cgccgcctga ttaacgtttt tgtacaccat ataacagacg ctgggcagcg
    34261 tcagggagat ggcgataacc atcaccgtta aaaacgtggc gaacggtttg cttttcagat
    34321 cctgcaatgc gccgtggaag gcatagcgca cctgttcgtt gaaaacgttg gttttgcgat
    34381 ttaccggttt tggcgaggat ttcgcgcgtt tcggtgcgtt acgaccgccg tcgcctgagc
    34441 cgccgaccga tttacggaag cgatccagac gcccaccaaa ctgccgaata tgattgattg
    34501 catcgcgctt attcatggcc cacgcctcca tgcaagtgac catcgctcag ggtgagcatg
    34561 cgataggaac gccgtgaaat cagattaatg tcgtgcgttg ccatcaatac ggttacccca
    34621 acgcggttaa actcttcaaa cagacgtaga atcccttccg acaacgcgtc gtccaggtta
    34681 ccggtcggtt cgtccgccag cagtaccgcg ggcttattca ccaccgcgcg ggcaatgcca
    34741 acacgctgtt gttcaccgcc ggaaagctga atagggaagt tcttcgcttt gtccagtagc
    34801 ccgactttat ccagtgccgc cgacacccgg cgacgaatat cgtcaccgct ggcaccagcg
    34861 ataatcagcg ggatcgccac gttatcgtag acagtacggt ccatcagtag atggtgatcc
    34921 tggaaaatca tgccaatctg gcggcgcaga aacggaactt cacggttttt cagacgcgtg
    34981 atgtcatggc cgctaaacca gattttcccg gcgctgggcc gctcaatccc acagatcagc
    35041 ttcaggaggg tacttttccc tgcgccggaa tgaccggtca gaaacgccat ctcacccggc
    35101 tgcatatgga acgtaacgcc ctgcagcgcc tgtctcccac cgagataagc cttgctgaca
    35161 tgttcaaagc gaatcattgt taatcctctc gggcaaaaag tgcctctata aagtcgtccg
    35221 ccttaaacgg acgcaaatcc tcaatacgtt cgccgacacc aatgtagcgg atagggatac
    35281 caaactggtc agccaccgag aaaattaccc cgcctttcgc cgtgccgtcc agtttcgtta
    35341 gcgtgatgcc ggttaagcca acagcttcgt ggaacagttt ggcctggctt accgcgttct
    35401 gcccggtgct ggcatcaata gtcagcataa cttcatgcgg cgcttcaacg tcgagtttct
    35461 tcatcacgcg gacgattttc ttcaactctt ccatcagatg cgatttgttc tgcagacgtc
    35521 cggcggtatc ggcaatcagg acgtcgatat tacgcgcttt agctgcctga atggcgtcga
    35581 agataacaga ggcggaatcc gcaccggtat gctgggcaat caccggaata ttgttgcgct
    35641 gaccccagac ctgaagctgt tcaaccgcag ctgcacggaa agtatcgccc gccgccagca
    35701 tcaccgattt accctgctgc tcaaactgac gcgccagctt accaatcgtc gtggttttac
    35761 ccacaccgtt gacgcccacc atcaggatca cgaacggcgt tttatcttca acattcagcg
    35821 gctcatcgac tttcgccaga atctcgccca tctcttcttt cagcaggcca tagagcgcct
    35881 cggcatcacg aagctgcttg cgggatgcgc cttccgtcag attggtgata attttacgtg
    35941 tggtttccac gcccacatcg gcgatcaaca gctgttcttc cagctcttca aacagatcat
    36001 cgtcgatttt tttaccgcgg aacaggctga taaatccgga accgagatt
//
LOCUS       DAJTSI010000022        35755 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000022 DAJTSI010000000
VERSION     DAJTSI010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.022"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..246
                     /locus_tag="OO327_001013"
     CDS             <1..246
                     /locus_tag="OO327_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR6017643.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(293..1051)
                     /gene="deoR"
                     /locus_tag="OO327_001014"
     CDS             complement(293..1051)
                     /gene="deoR"
                     /locus_tag="OO327_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR6017644.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="OO327_001015"
     CDS             complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="OO327_001015"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6017645.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1990..3216
                     /gene="mdfA"
                     /locus_tag="OO327_001016"
     CDS             1990..3216
                     /gene="mdfA"
                     /locus_tag="OO327_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HCR6017646.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3257..3541)
                     /locus_tag="OO327_001017"
     CDS             complement(3257..3541)
                     /locus_tag="OO327_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6017647.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="OO327_001018"
     CDS             complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="OO327_001018"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR6017648.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4442..5650)
                     /locus_tag="OO327_001019"
     CDS             complement(4442..5650)
                     /locus_tag="OO327_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6017649.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5734..6270
                     /gene="rcdA"
                     /locus_tag="OO327_001020"
     CDS             5734..6270
                     /gene="rcdA"
                     /locus_tag="OO327_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR6017650.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6445..8130)
                     /locus_tag="OO327_001021"
     CDS             complement(6445..8130)
                     /locus_tag="OO327_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR6017651.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8400..8777
                     /locus_tag="OO327_001022"
     CDS             8400..8777
                     /locus_tag="OO327_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR6017652.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="OO327_001023"
     CDS             complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="OO327_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR6017653.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9224..9511
                     /locus_tag="OO327_001024"
     CDS             9224..9511
                     /locus_tag="OO327_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR6017654.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            9495..10217
                     /gene="nfsA"
                     /locus_tag="OO327_001025"
     CDS             9495..10217
                     /gene="nfsA"
                     /locus_tag="OO327_001025"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCR6017655.1"
                     /translation="MTPTIELICGHRAIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10278..11180
                     /gene="rimK"
                     /locus_tag="OO327_001026"
     CDS             10278..11180
                     /gene="rimK"
                     /locus_tag="OO327_001026"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR6017656.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11268..11744
                     /locus_tag="OO327_001027"
     CDS             11268..11744
                     /locus_tag="OO327_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR6017657.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12096..13208
                     /gene="potF"
                     /locus_tag="OO327_001028"
     CDS             12096..13208
                     /gene="potF"
                     /locus_tag="OO327_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR6017658.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13303..14436
                     /gene="potG"
                     /locus_tag="OO327_001029"
     CDS             13303..14436
                     /gene="potG"
                     /locus_tag="OO327_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR6017659.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14446..15399
                     /gene="potH"
                     /locus_tag="OO327_001030"
     CDS             14446..15399
                     /gene="potH"
                     /locus_tag="OO327_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR6017660.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15396..16241
                     /gene="potI"
                     /locus_tag="OO327_001031"
     CDS             15396..16241
                     /gene="potI"
                     /locus_tag="OO327_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR6017661.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     GGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16301..16789
                     /locus_tag="OO327_001032"
     CDS             16301..16789
                     /locus_tag="OO327_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR6017662.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16830..17957
                     /gene="rlmC"
                     /locus_tag="OO327_001033"
     CDS             16830..17957
                     /gene="rlmC"
                     /locus_tag="OO327_001033"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR6017663.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18132..18863)
                     /gene="artJ"
                     /locus_tag="OO327_001034"
     CDS             complement(18132..18863)
                     /gene="artJ"
                     /locus_tag="OO327_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR6017664.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19155..19823)
                     /gene="artM"
                     /locus_tag="OO327_001035"
     CDS             complement(19155..19823)
                     /gene="artM"
                     /locus_tag="OO327_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR6017665.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19823..20539)
                     /gene="artQ"
                     /locus_tag="OO327_001036"
     CDS             complement(19823..20539)
                     /gene="artQ"
                     /locus_tag="OO327_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR6017666.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20546..21277)
                     /gene="artJ"
                     /locus_tag="OO327_001037"
     CDS             complement(20546..21277)
                     /gene="artJ"
                     /locus_tag="OO327_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6017667.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21295..22023)
                     /gene="artP"
                     /locus_tag="OO327_001038"
     CDS             complement(21295..22023)
                     /gene="artP"
                     /locus_tag="OO327_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR6017668.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22241..22756)
                     /locus_tag="OO327_001039"
     CDS             complement(22241..22756)
                     /locus_tag="OO327_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6017669.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23405..25174
                     /locus_tag="OO327_001040"
     CDS             23405..25174
                     /locus_tag="OO327_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR6017670.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNPSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25385..25708
                     /locus_tag="OO327_001041"
     CDS             25385..25708
                     /locus_tag="OO327_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR6017671.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25705..26535
                     /gene="amiD"
                     /locus_tag="OO327_001042"
     CDS             25705..26535
                     /gene="amiD"
                     /locus_tag="OO327_001042"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR6017672.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26532..27545)
                     /locus_tag="OO327_001043"
     CDS             complement(26532..27545)
                     /locus_tag="OO327_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR6017673.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27644..29074)
                     /locus_tag="OO327_001044"
     CDS             complement(27644..29074)
                     /locus_tag="OO327_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR6017674.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29085..30086)
                     /gene="ltaE"
                     /locus_tag="OO327_001045"
     CDS             complement(29085..30086)
                     /gene="ltaE"
                     /locus_tag="OO327_001045"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR6017675.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30123..31841)
                     /gene="poxB"
                     /locus_tag="OO327_001046"
     CDS             complement(30123..31841)
                     /gene="poxB"
                     /locus_tag="OO327_001046"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR6017676.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31974..32942)
                     /gene="hcr"
                     /locus_tag="OO327_001047"
     CDS             complement(31974..32942)
                     /gene="hcr"
                     /locus_tag="OO327_001047"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR6017677.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32954..34606)
                     /gene="hcp"
                     /locus_tag="OO327_001048"
                     /gene_synonym="priS"
     CDS             complement(32954..34606)
                     /gene="hcp"
                     /locus_tag="OO327_001048"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR6017678.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(34750..35649)
                     /gene="lysO"
                     /locus_tag="OO327_001049"
     CDS             complement(34750..35649)
                     /gene="lysO"
                     /locus_tag="OO327_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR6017679.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         8912 a         8838 c         9188 g         8817 t
ORIGIN      
        1 gtgaccatac cgcgtgggca gctgaaaaac ctgaaggcga gctacacgtt aacggaaccg
       61 cagcttaccg caccgctgaa aaaaggtcag gttgtcggga ccattgattt ccagcttaac
      121 ggtaaatcca ttgagcagcg tccgctgatc gtgatggaaa atgtggaaga gggcggattc
      181 tttggtagga tgtgggattt cgtgatgatg aaattccatc agtggttcgg cagctggttc
      241 tcttaatctt ctgataaccg gatggcgcga aacgtcatcc ggttatacgt cattaataca
      301 tcaacttaat acggtgcgtc tgcgcgtact tcacatactc atcttccggg caacaatcac
      361 tcaccacaat atcaaagcgt ttcaggtcac ccatgcgcgc cggacgcacc ttgccaaatt
      421 tactgtggtc gacaaccagc acatgctttt gcgccatcga catggcccag tgttttaccg
      481 gcaactcttc aagattaaaa caggtagcgc ctttactgac atgcacgccc gccgcggaat
      541 aaaaagcgat atccgggcaa aaattattca acgtttgctg aaaatcgatg ggtttgaaaa
      601 tggcgttgct ggcgtgaaat tcaccaccgc acagaaatgc gcggcaatgg ggtttctctt
      661 tcagcgccag aaaggtattt agcgaataac aaacggcggt aaaagggatt tcattatcaa
      721 tcgcttcaat aatccacggc gtggtggtgc cacagtcaaa aaagagggtc tgatcgggtt
      781 ctaccagtgt cgcagccagt tttgccgccc ggcgtttttc ttccaccagg cgggattttt
      841 gatcgcttaa caggtaatgg ctggcactgc gcggttccag aacaatatag ccgccgagca
      901 aaacgacggg cgcactgtgg ttgttcagat cgcgacgaat cgtcatctcc gaaaccccaa
      961 gcagggcggc ggcgtcttta agatgtaact tatcgctgcg ttttaattct tgcagcagct
     1021 gcccgatacg ctcttcgcga cgtgtttcca taatccctct gaatagttat tgaagcgagc
     1081 cgctcaatac tacacttttt agcagagatc agtcacgcac ccagcctttg cggatcggta
     1141 atgcaaaaca gacgcgatac cacgattgca gacgttgata gagtttatgc cccatcgctt
     1201 gccagataat ctgggcacta aggcagccaa tcatacctgc cagcaatcca ccgagcatat
     1261 ccagcggcca gtggacgcca agataaacgc gcgaccaggc aatgacgacg gccagcacca
     1321 ttaaaagtga gccggaccac aggcgatgcc agcataaaaa tgccagtgca aaggtgaaaa
     1381 tcaccgtacc gtgatcgctc gggaatgagt catccgccgc atgatgcagg aagttatagc
     1441 cgatattttc gacaaagggt cggtcgtgcg gaaaaagatg tcccatcgtc caggacacaa
     1501 acaggctgac cgccagcgcg atagcgattt ttatcaccag ttgccgttgt gctgtaagcc
     1561 cccacaacca aagtaccgcg gccagcaacg gcaccacggt aatcaaatct ttagcaataa
     1621 aaatcgccaa cgagatcatc cacggagccg agtctggcgt cgcgttaata agagagaata
     1681 gagagagatt caaattttcc agcataactt cccgacgcaa agtgattaaa agggagccaa
     1741 tacaggcaag tcgttgagaa taaagtgcag gttaaactgg gtaaagcggc atcgtcttat
     1801 ttccctcaag cggcctgttt acggtgggtg attgtaacgg gcataggtta aataaaactt
     1861 aaagaaagcg tagctatact cgcaataatg taagaatgtg cttaaccgtg gtttcagcta
     1921 caaaattcgc tttctcgtta gctgcgcttt tattaaactc tgcgcgatta ttattggcga
     1981 agaaattgca tgcaaaataa attagcttcc ggtgccaggc ttggacgtca ggcgttactt
     2041 ttccctctct gtctggtgct ttacgaattt tcaacctata tcggcaacga tatgatccaa
     2101 cccggtatgt tggccgtggt ggaacaatat caggcgggca ttgattgggt tcctacttcg
     2161 atgaccgcgt atctggcggg cgggatgttt ttacaatggc tcctggggcc gctgtcggat
     2221 cgtattggtc gccgtccggt gatgctggcg ggtgtggtgt ggtttatcgt cacctgtctg
     2281 gcaatattac tggcgcaaaa cattgaacaa ttcaccctgt tgcgcttctt gcagggcata
     2341 agcttctgtt tcattggcgc tgtgggatac gccgcaattc aggaatcctt cgaagaggcg
     2401 gtttgtatca agatcaccgc gctgatggcg aacgtggcgc tgattgctcc gctacttggt
     2461 ccgctggtgg gcgcggcgtg gatccatgtg ctgccttggg aggggatgtt tgttttgttt
     2521 gccgcattgg cagcgatctc ctttttcggt ctgcaacgag ctatgcctga aaccgccatg
     2581 cgtataggcg agaaactgtc actgaaagaa ctcggtcgtg actataagct ggtgctgaag
     2641 aacggccgct ttgtggcggg ggcgctggga ttcgttagcc tgccattgct ggcgtggatc
     2701 gcccagtcgc cgattatcat cattaccggg gagcagttga gcagctatga atatggcttg
     2761 ctgcaagtgc ctattttcgg ggcgttaatt gcgggtaact tgctgttagc gcgtctgacc
     2821 tcgcgccgca ccgtacgttc gctgattatt atgggcggct ggccgattat gattggtctg
     2881 ttggtcgctg ctgcggcaac ggttatctca tcgcacgcgt atttatggat gaccgccggg
     2941 ttaagtattt atgctttcgg tattggtctg gcgaatgcgg gactggtgcg attaaccctg
     3001 tttgccagcg atatgagtaa aggtacggtt tctgcggcga tgggaatgct gcaaatgctg
     3061 atctttaccg tcggtattga aatcagcaaa catgcctggc tgaacggggg caacggactg
     3121 tttaatctct tcaaccttgt caacggaatt ttgtggctgt cgctgatggt tatcttttta
     3181 aaagataaac agatgggaaa ttctcacgaa gggtaaaaaa atgcctgatt gatttgtgcg
     3241 atcaggcatt ctcgaattaa tggtgatggt cgtcaatctg gtgttcgata accatccctt
     3301 cacctacgct ggcaagatgg cgaacataag gatgcgggcg gtaagccgga gctggcgcag
     3361 gagccacata gacggtttgc ggcacagtcg cgacgctgac cgcttgtgga acgctgactg
     3421 aatcaggaac cacaaccact ttgtatccac tcggcacatc gacggtgaca ctttgcgcca
     3481 tcgccacacc agtaaagccc attaaaagcg cgcccaacag tagacaattt ttcataatca
     3541 tttcgcctga aataatgcag cgggttaatt tccgcgaatt atgcagcctt cattcaggca
     3601 ttgtgttgcc gggcgtgctg ccgtttttat tggtcaaatg gcgcttcgtg tttaagaact
     3661 ttatcgatca catccagtac gccttcacgg ttattgtagc ctgcccggta ttttgccgct
     3721 gcgacgactg cgctgccggc attttccatt gcaaaactaa agcctgcctg acgcagcatc
     3781 tcaatatcgt taccgccatc gccaaagacc accacttcgc tgtcgtctat tccccataat
     3841 ttctgcagtt ggcgaaggcc attggcttta tgtacgccgg ggataatcag atcgatgctg
     3901 ccgttgccgg tgtggactgg caccataata tcgccgatgg cctcatgtaa tgctttttgt
     3961 acttgtggaa tcagttcatc ggaaagattc aggccaaact taaagaaaat atcttctaag
     4021 ttgtcaaagt tatcgacgta ttccagacgg tgataataca tttccgccac cgttttcatg
     4081 gcatcgtcat attttttgag tgtataggcg ctattttttc cgcaggcaat aatttccact
     4141 tccgggcgcg tcagcaaatg ttccacgaca gtagcaaacg catccttcga cagctcgcca
     4201 ttaaaaacat ctttgccttc actcattacc cagccgccgt tttcagccac aaaggcaatt
     4261 tcattcgcaa tttcagggaa gaaagagatc aactgataat attgattccc gctggcgacc
     4321 acaaagcgaa tgccttgtgc tttcatttgc tgatactgag ccataaaccg ctcacggtta
     4381 taggtttttt gatcgcttaa gaaagtacca tccatgtcta ccgcaattaa tttaatgctc
     4441 atcaactatt ctccatcgcc gtctgcgttt tggtatcggg tttggcgacg gctttcgcga
     4501 caatagccgc gagaataacc agcgccagta caaccagcat tgcactacgt aatccataat
     4561 gttcgccgag atagcccagc agcggcggcc cgacgaggaa agccagataa ccggtcgttg
     4621 ctaccacact gacgcgggtt ggtgcatcgg ggccggtatc gctggcggca gaaatggtca
     4681 gcgggaagcc cagcgaggca cccagtcccc aaagtacaac agacactcct gcgacccagg
     4741 cgctatcgac aaaaataatc agcccaatac ccaacgcccc cattagtgca ctggcccgaa
     4801 ccacggcaac gcgactgtaa ctgtcgatga accaaccgcc ggtgaagcgt ccaacggtca
     4861 tccccagggt aaaaccggca taaatcagtg agccggaagt ggggctaaaa ccgtgaccat
     4921 caaccattaa taagggtaac cagtcgttgg cagaaccttc ggcaaaggcc atcgccagca
     4981 ctacaacacc aatcaacagc aactggatat cgcgataaaa aggaatgcct ttttcgccat
     5041 gctgggtgcc atcggcagca tttttgcccg taccgtcagg gattgcctga atggcgatat
     5101 aaataggcgc gatacctacc agcgccgcca ataaaatgtg caccgttgcc ggaacgccaa
     5161 aggccgtcag tgccatcccg acaccagcgc ctgccagcgt gcccaggcta taaaaaccgt
     5221 gcatcatcgg caaaaccgtt ttattcattt ctcgctcaac ggcggcacct tcaacgttta
     5281 tcgccacttc cgcagaacca aaacttgccc caaagacgcc gagaccaacg gcaaagagca
     5341 ggggcgatgt cagccagagt gccagactta atatcatcat cccgatcaat gcgcaggaca
     5401 tcgtgaccag gatgacatta cgtgtcccaa agcgtttcac taaccacgcc gagcagagaa
     5461 taccgctcat cgaaccgatc gacagaccaa agagaacacc gcccatttca gcgatcgaga
     5521 cagagagaat atcgcggata gcaggcgtac gggttgccca ggacgccatt aacaggcctg
     5581 gcaaaaagaa gaacataaac agcgcccagg ttcggcgttt caatgcatta cgtgaagaat
     5641 ttacggtcat agatcacgtc aaaataagaa gagggaagac aacattagca aggttgtgta
     5701 catttgtaca caattgcaga cagaggaaat gacatgcgtc gcgctaacga tccgcaacgg
     5761 cgagaaaaaa ttatccaggc cacgctggag gcggtgaaac tttacggaat acatgctgtt
     5821 acgcaccgca aaattgctac ccttgccggg gtaccgttgg gatcgatgac ctactatttt
     5881 tcaggaattg atgagttgtt gctggaggcg ttcagcagtt ttactgagat tatgtcccgg
     5941 caatatcagg cattttttag cgatgttagt gatgctcaag gcgcatgcca ggctatcacc
     6001 gatatgatct acagctcaca ggttgcaacg ccggataata tggagctgat gtaccagctc
     6061 tacgcgctgg ctagccgaaa accgctatta aaaacggtaa tgcaaaactg gatgcagcgc
     6121 agtcagcaaa cgctcgaaca atggtttgaa cccggaaccg cccgcgcgct tgatgcgttt
     6181 attgagggga tgacgctgca ttttgtcacc gaccgtaagc cgctatcgcg cgaggagatt
     6241 ttgaggatgg ttgagagggt tgcagggtag tagataaatt ttagataaca aaaaacccat
     6301 caaccttgaa ccgaaatggc ggggttgatg ggctccacaa aatggggaca tcaaagaaaa
     6361 gcagtggcac taattaagac tgatgccctg cggaaaagtt ctgcggttgt gcaaaaaaat
     6421 ttcattttca gggcaacttc agttttatcc taatcctggc cataccatga cgatgattgt
     6481 ccctgccagc gtcagcagga cgttggcgat tgcgtaggtg cccgcatagc ccagcgcagg
     6541 gatgttactg cgagctgtat cactgatgat ctccattgcc ggcgcgcagg tgcgtgcgcc
     6601 catcattgcg ccgaacaaca gtgcgcggtt cattcgcaat acataagcac cgaacaagaa
     6661 acagataacc acgggcacaa gactgacaat taatccggca atcaacatct gaccgccaat
     6721 cgcgcccagg ccgttattaa taccgctacc ggcgctcaga ccaacgcctg ccataaacac
     6781 catcaagccg aactctttca ccatgcttaa tgcaccttgc ggaatgtaac cgaaggtcgg
     6841 gtggttagca cgcataaagc ccagcataat tccggcgaat aacaacccgg cagcgttccc
     6901 catgccgaaa ctgaatgtgc tgaactggaa ggtgatcatc ccgatcatca gcccaataac
     6961 aaagaaggcg caaaatgcca gcaggtcagt gacctggctg tgaatcgaga taaagccgat
     7021 gcgatcggcg atggttttta cgcgacgggc atcaccgctg acttgtaaaa cgtcaccttt
     7081 gttaagcacg acgttgtcat ctatcggcat ctcaatctgg ctacgaatga cgcggttaag
     7141 gaagcaaccg tgatcggtca acttcagttg tgcgagacgt ttacctacag cgttatggtt
     7201 tttaacgacc acttcttcag tgacgatacg catgtcgaga aggtcacgat cgaaaacttc
     7261 tttaccgtta cggaagctgg gatcgagtcg ggcatgggcg tcgggatagc ctaccaacgc
     7321 tatttcatcg cccatttgta gcacggcatc accgtctgga tttgccagaa tcccgttacg
     7381 tcgaatacgt tcaatgtagc agccggtttg tcgataaata cccagttcac gcagattttt
     7441 gccgtcggtc caggccacca gctccgggcc gacgcgatag gcgcggatca ccggtaaata
     7501 aaccttacgg ttggcatcag tgtccaggcc acgttcgcgg gcgatttgct gggcgctggt
     7561 ctgtaagtcc tgatgctgca atttcggcaa gtaacgcgca ccaacaatca aactcaccag
     7621 accgattaaa taggttaagg catacccgag gctcagatta tccagtgcca gtgagagctg
     7681 cctgctttcc atgccggaat gatgcagtgt atcgccagca ccgaccagaa ccggtgtcga
     7741 cgtcatagag cctgctaaca taccggccgt caggccaata tcccagccaa acagcttacc
     7801 taaccctaag gcgatcacca gcgcactgcc aaccatcacc agtgctaaca ttaggtaatt
     7861 tttcccatcg cgaaaaaaaa tggaaaaaaa gttcggtccg gcttcgaccc cgacgcagaa
     7921 aataaacagc ataaagccaa gattaagcgc atcggtgtta atgctgaaat gttgttggcc
     7981 taataacagc gatacgacta aaacgccaat ggaattaccc agttggatcg aaccaagtcg
     8041 taactttccg agacatagcc caagcgcgag gaccacaaat aataacagaa tgtaattccc
     8101 atttaacaat tcggcgacgt ttatattcac ggaggctaac ttcttgttta ctagtaagct
     8161 gttgaaagaa atgctaattt acgataatgt gttttaccag aattcagggc gcagattcat
     8221 tcagcgcacc taaacgatag taaagtaaca atatatttta ctagtgtaat cacattaggt
     8281 atcaacggct atatgaattg cgttggccta tattagcatg gaatgcgaag cggctttatc
     8341 ttactgaacg ccacactggc gaaaaatgtg ttcgatagac gcagtgtcag gaggaacgag
     8401 tgaaacataa acaacgttgg gcgggggcaa tctgctgttt tgtcctcttc attgtggtgt
     8461 gcctttttct ggcgacgcac atgaaaggcg cttttcgggc tgccgggcat cctgaaatcg
     8521 gcttgctgtt tttcattctt cctggagcag ttgccagttt cttttcacag cgtagagaag
     8581 tcctgaaacc tctatttggc gcaatgctgg cggcaccctg ttcaatgctc attatgcggc
     8641 tgtttttttc accgacgcgc tcattctggc aagagctggc atggttacta agcgcggtgt
     8701 tctggtgtgc gctgggggca ctgtgtttct tatttatcag tagtttgttt aaaccacagc
     8761 acagaaaaaa tcagtaaagc cctcaacgcg agggcttgtc agacgatcag gcgtccagat
     8821 tttctttcac ccatgcagca aaatcggtat agccgccgat atgttgctga tcgacaaaaa
     8881 tctgcggcac ggtttctacg ggtttacctg ccttttgttg tagatcttct ttagtgatcc
     8941 cttccgcacg aatatctaca tactgatact gaaaatcatc gcgttcattg ctcaatttct
     9001 cagccagatc ttttgcacgc acacagtaag ggcaacccga acgaccaaaa ataacggttt
     9061 gcattatttc tctcctcata gatttatgcc tgtaatgatc acgctaaaat gtattcgctg
     9121 aaagtaggtt taacctgttg cattaattgc taaaagctat aactgttaaa cacaatacag
     9181 tgaaaagttt tagactgaag gctcactttg cagagggaag cgtatgcgcg cgatcggtaa
     9241 attgcctaaa ggcgtgttga tactggaatt tatcggaatg atgctactgg cggtggcgct
     9301 gctgtcggta agcgactccc tgtcgctgcc tgagccattt tctcggccag aagtgcagat
     9361 tctgatgatt tttctcggtg ttttgctcat gcttcccgct gcggtggtgg ttattcttca
     9421 ggtggcaaaa cgtcttgccc cacagctgat gaactgtcca ccgcaatatt cacgttcaga
     9481 aagagaaaaa gataatgacg ccaaccattg aacttatttg tggccatcgc gccattcgcc
     9541 atttcactga tgaacccatt tccgaagcgc agtgtgaggc gattattaac agcgcccgtg
     9601 cgacgtccag ttccagtttt ttgcagtgta gtagcattat tcgcattacc gacaaagcat
     9661 tacgtgaaga actggtgacg ctgaccggcg ggcaaaaaca cgtagcgcaa gcggcggagt
     9721 tctgggtgtt ctgtgccgac tttaaccgcc atttacagat ctgtccggat gctcagctcg
     9781 gcctggcgga acaactgttg ctcggtgtcg ttgatacggc aatgatggcg cagaatgcat
     9841 taaccgcagc ggaatcgctg ggattgggcg gggtatatat cggcggcctg cgcaataata
     9901 ttgaagcggt gacgaaactg ctgaaattac cgcagcatgt tctgccgctg tttgggctgt
     9961 gccttggctg gcctgcggat aatccggatc tcaagccgcg tttaccggcc tccattttgg
    10021 tgcatgaaaa cagctatcaa ccgctggata aagacgcact ggcgcagtat gacgagcaac
    10081 tggcggaata ttacctcacc cgtggcagca ataatcgctg ggatacctgg agcgatcata
    10141 tccgccgaac aatcattaaa gaaagccgcc catttattct ggattatttg cacaaacagg
    10201 gttgggcgac gcgctaaaac cgccacgtcg atgtatgata cgcgggcttt tgaccaggtc
    10261 tgacagagag gtgcagggtg aaaattgcca tattgtcccg ggatggaacg ctctattcgt
    10321 gtaagcggct gcgtgaagcc gctatacagc gcggtcacct ggttgaaatt cttgatccgc
    10381 tttcttgcta catgaacata aatcctgcgg cgtcttctat ttactacaaa ggccgcaagt
    10441 taccccattt tgacgcagtg atcccgcgta ttggcaccgc cattaccttt tatgggacgg
    10501 cggcactgcg ccagttcgag atgctgggga gctatccgct caatgagtcg gtcgccattg
    10561 cccgggcgcg tgacaaattg cgttccatgc aactgctggc gcgtcagggc atcgacctgc
    10621 ctgtcacggg cattgcgcat tcgccggatg ataccagcga tttaatcgac atggtcggtg
    10681 gtgcgccgct ggtggtcaag ttggttgaag gcacgcaggg aattggcgtc gtgctggcgg
    10741 agactcgtca ggcggcggaa agcgtgattg acgctttccg cggtctgaac gcgcatattc
    10801 tggtgcagga atatatcaaa gaggcgcaag ggtgcgatat ccgctgtctg gttgttggtg
    10861 atgaagtggt cgctgcgatt gaacggcggg cgaaagaggg ctattttcgt tccaatttgc
    10921 atcgtggcgg cgcggcaagc gtcgccagta tcacaccaca ggagcgtgaa atcgcgataa
    10981 aagccgcgcg aacgatggcg ctggacgttg ctggtgtgga tattctgcgt gctaatcgcg
    11041 ggccgttggt gatggaagtg aatgcgtcgc cggggctgga aggaatagaa aaaaccaccg
    11101 gtatcgatat cgcgggtaaa atgatccgct ggatcgaacg ccacgctacg acagaatatt
    11161 gcctgaaaac gggtggttag tcgcaatcac attactgatc atggttttgc ctgcgctttt
    11221 tgcgtaagct gtgccggtct ttttatcgaa agaggttgta caaaattatg acatcgctgg
    11281 tcgttcctgg tctggatacg ctgcgtcaat ggctcgatga cctggggatg agtttttttg
    11341 aatgtgataa ctgtcaggct ctgcatctgc cccatatgca gaatttcgac ggtgtctttg
    11401 atgccaaaat cgatctgatc gataacacga tcctgttttc tgccatggcg gaagtccgac
    11461 cttcagccgt attgccgctg gcggcggatt tatctgccat caatgccagt tcgctgaccg
    11521 tgaaagcatt tcttgatatg caggatgata atctgccaaa gctggtggtt tgccagtctt
    11581 tatccgttat gcagggcgta acctatgagc agtttgcatg gttcgtgcgt cagagcgaag
    11641 agcagatttc gatggtcatt cttgaagcta atgcccatca actgctgtta ccgactgacg
    11701 atgaagggca aaacaacgtt accgaaaact attttctcca ctgataattc ctttcgagca
    11761 cgcagtcgct gccgcagtga ctgcgcgctg caaaattatc tgctgttttt aaccttttct
    11821 taaagattat ttcacttctc ttgtgtcgat ttggctttat cacatagagc aaatacgcat
    11881 aaaaatttgt taaataccgt tttttaatcc gagctatagt ctcaaaccct ggctaaagtt
    11941 attcttgcga tgcttctata tagcgagcag tgctggccgg gagagagttc tcttttctta
    12001 caccgcgccg ataaaaaaat atgcacgttt attgcatatc tttcagtgtg acaacttttg
    12061 ttcgtttgtt aacgaacttt cagaaggaaa gagatgtgac cgccttaaat aaaaaatggc
    12121 tatcgggtct ggttgcgggt gctctgatgg ccgtctctgt cggcacgctc gcggctgaac
    12181 aaaaaacact ccacatttat aactggtctg attatatcgc ccctgacacg gtggccaatt
    12241 ttgaaaaaga aaccggtatt aaagtcgtct acgatgtttt cgactctaac gaagtactgg
    12301 aaggcaaatt aatggcgggt agcaccggct ttgacttggt ggttccatct gccagctttc
    12361 tagaacgcca gttgactgcg ggagttttcc agccgctgga caaaagcaaa ttgccggagt
    12421 ggaagaatct cgacccggag ctgctgaagc tggtcgccaa acacgatccc gacaataaat
    12481 ttgctatgcc ctatatgtgg gcgacgaccg ggattggcta taacgttgat aaagttaaag
    12541 ctgtgctggg cgaaaacgcg cccgtcgata gctgggactt gatcctcaaa cctgaaaatc
    12601 tggaaaaact gaaaagctgc ggtgtctctt tcctggatgc gccagaagaa gtttttgcta
    12661 ccgtgttgaa ttatctcggc aaagatccca acagcactaa agcggatgat tacaccggac
    12721 cggcaaccga tctgctgtta aagctgcgcc cgaacattcg ttatttccat tcatctcaat
    12781 acattaacga cctggcaaac ggcgatattt gcgtcgctat cggctgggca ggtgatgtct
    12841 ggcaggcgtc aaaccgcgcg aaggaagcga agaatggagt gaatgtctcg ttctcgattc
    12901 caaaagaagg ggcgatggcg ttctttgatg tattcgccat gccagcggat gccaaaaaca
    12961 aagacgaagc ctatcagttc ctgaattacc tgctgcgccc ggatgtagta gcgcatattt
    13021 ccgaccatgt gttttatgtc aacgccaata aagcagccac gccgctggtg agtgcggaag
    13081 tccgtgagaa cccgggtatt tatccgcctg cggatgttcg tgcgaagctg ttcactctga
    13141 aagtgcagga cccgaaaatc gaccgtgtgc gcacccgcgc gtggaccaaa gtgaagagcg
    13201 gaaaataatc cgcagcctta gatgccgggc gggcgcacca cacccgccgg caattcgcac
    13261 catcatggtg cgcttgcaca cattcaatgc cggagagcag ccgtgaatga cgctatccct
    13321 cgcccgcagg cgaaaacccg taaggcgctg acgccgctat tagaaatccg caacctcacc
    13381 aaatcctacg atggtcaaca tgcggtggat gatgtcagcc tgaccatcta caaaggtgaa
    13441 atcttcgcgc tgctgggcgc atccggctgt ggcaagtcca cgctgctgcg tatgctggca
    13501 ggtttcgaac aaccttctgc cggacagata atgcttgatg gcgtcgattt gtcacaggtt
    13561 ccgccttacc tgcgccccat caatatgatg tttcagtctt acgcgctgtt tccgcatatg
    13621 accgtggaac agaacatcgc ttttggcctg aaacaggaca aactaccgaa agcggaaatt
    13681 tccagtcggg tcaatgagat gctcggcctg gtgcacatgc aggagttcgc caaacgtaaa
    13741 ccgcatcagc tttctggtgg tcagcgacag cgtgtggccc tggcccgaag ccttgccaag
    13801 cgcccgaaac tattactgct cgacgagccg atgggcgcgc tggataaaaa gctgcgtgac
    13861 cggatgcagc ttgaagtggt ggatattctg gagcgcgtcg gtgtgacttg tgtgatggtc
    13921 acccacgatc aggaagaggc gatgaccatg gcggggcgca tcgccattat gaatcgtggg
    13981 aaatttgtcc agattggcga accggaagag atctacgagc atccgactac ccgctatagc
    14041 gccgaattta ttggctcggt aaacgtcttt gaaggcgtac tcaaagagcg tcaggaagat
    14101 ggcttggtgc ttgattcgcc ggggctggtg catccactga aagtcgacgc ggatgcctcg
    14161 gtggtcgata acgtgccggt acatgtggcg ctgcgcccgg aaaaaatcat gctttgcgaa
    14221 gagccgcccg ccaatggttg taacttcgcg gtgggggagg tgatacacat tgcctatctc
    14281 ggcgatcttt cggtgtatca cgttcgtctg aaaagtgggc agatgattag tgcccagcta
    14341 caaaacgccc atcgtcatcg taaagggtta ccgacctggg gcgacgaagt gcgtttgtgc
    14401 tgggaagtgg acagctgtgt ggtgctgacg gtttaaggag caaagatgaa tacacttgaa
    14461 cctgctgccc agtcgaaacc gccgggcgga tttaagctgt ggttgtcgca gctgcaaatg
    14521 aagcatgggc gcaaactggt cattgcgttg ccatatatct ggttgatctt gctgtttctg
    14581 ctgccatttc tgattgtctt taaaataagc ctggcggaga tggcgcgcgc tattccacct
    14641 tataccgaac tgatggagtg ggctgacggg caactgtcca tcactcttaa tctcggtaat
    14701 tttctgcaac tgaccgacga tccgctctat ttcgatgctt atctccagtc gctacaggtg
    14761 gctgcgattt cgacattttg ctgtttactg atcggctatc cgctggcgtg ggcggtggcg
    14821 cacagtaagc cttcgacccg taatatttta ttactactgg tgatcctgcc gtcgtggacc
    14881 tcgtttctga tccgcgttta tgcttggatg ggaatattaa aaaacaacgg tgtgctgaat
    14941 aattttctgc tgtggctggg ggttatcgat caaccgctga ccattcttca taccaatctg
    15001 gcagtttata ttggcattgt ttacgcctac gtgccgttta tggtgctgcc gatttatacc
    15061 gcgttgattc gtattgatta ttcgctggtg gaagcagcgc tggatctcgg tgcacgaccg
    15121 ctgaaaacgt tctttactgt gatcgtgccg ctgactaaag gtgggattat tgccggatcg
    15181 atgctggtgt ttatcccggc tgtgggcgag tttgtgatcc cggaactgct cggtggcccg
    15241 gacagcatca tgatcgggcg cgtgctatgg caagagtttt tcaataaccg cgactggccg
    15301 gtggcctcgg cggtagcgat catcatgttg ctgctgctaa ttgtgccgat aatgtggttc
    15361 cacaaacacc agcaaaaaag cgtgggagaa cacggatgaa taatttaccg gtagttcgtt
    15421 cgccctggcg gattgtgatt ttgctgctgg gctttacctt tctctacgcg ccaatgctga
    15481 tgctggttat ctattcgttt aacagctcga agctggtgac ggtgtggggc ggctggtcaa
    15541 cgcgctggta tggtgagtta ttgcgcgatg acgcgatgat gagtgcggtt ggttcaagcc
    15601 tgacaattgc tgcctgtgcg gcaacggcgg cggcgatcct cgggactatt gcggcggtgg
    15661 tgctggtgcg ctttggcagg tttcgcggat caaatggctt tgcctttatg atcaccgcgc
    15721 cgctagtaat gccagatgtc atcacgggct tgtcgctgtt gttattattc gtcgcgcttg
    15781 ctcatgccat tggctggcct gcggaccgcg gtatgcttac catctggctg gcgcatgtca
    15841 ctttctgtac ggcttatgtg gcggtcgtta tttcgtcgcg tctgcgggaa ctggatcgct
    15901 cgatagaaga agccgcgatg gatctcggtg cgacgccgct gaaagtgttt ttcgtcatta
    15961 cgctaccgat gatcatgccc gcgatcattt ctggctggtt actggctttt actttgtcgc
    16021 ttgatgatct ggtgatcgcc agctttgttt ccgggccggg agccaccacg ttaccgatgc
    16081 tggtcttttc cagcgtgcgg atgggggtga atccggaaat caacgccctg gcaacattga
    16141 tcctcggcgc ggtcggaatt gtcggattta tcgcctggta tctgatggcg cgcgcagaaa
    16201 aacagcggat acgcgacatc caacgtgcaa gacgtggctg aagacactaa aatttgccaa
    16261 cctggctaca taatgccgcg catgtcgcgg cattgttttc atggaagacg aaacgttggg
    16321 attttttaag aaaacatctt catctcatgc tcgcctgaat gtgcctgcgc tggtgcaggt
    16381 ggcggcgctc gccattatta tgatccgtgg cctcgacgtg ctgatgattt tcaatacgct
    16441 gggcgtgcgc ggtattggcg agttcattca tcgcagcgta caaacctgga gtttaacgct
    16501 ggtcttttta agcagtctgg tgctggtttt cattgagatc tggtgtgcgt tttcactggt
    16561 gaaagggcgt cgctgggcgc gctggctata tctgctgaca caaatcaccg ccgcaagtta
    16621 cttgtgggcg gcttcgctgg ggtacggtta tccggagctg ttcagcattc ccggtgaatc
    16681 aaaacgtgaa atcttccata gcctgatgct gcagaagctg ccggatatgc tcatcctgat
    16741 gctgctgttc gttccctcga ccagtcggcg gttcttccag ttgcaataat gtgtataatc
    16801 gtcgcccctg atgatgtgaa ggtcaatgta tgcagtgcgc actttacgac gcgggtcgct
    16861 gtcgttcctg tcagtggata acgcagccga ttccagagca actctccgct aaaaccgccg
    16921 atcttaaaaa tctgctcgcc gattttccgg ttgaggaatg gtgcgcgccg gtgtcaggcc
    16981 cggaacaagg gtttcgtaat aaagccaaaa tggtggtgag tggtagcgtt gaaaaaacac
    17041 tgctcggtat gctgcatcgc gatggcacgc cggaagacct ttgtgactgc ccgctttatc
    17101 cagcctcatt tgcgcccgtt tttgcggcgc taaaaccgtt tatcgcccga gcggggttaa
    17161 cgccctacaa cgtggcgcgt aagcgtggcg aactgaaata cattctgctg actgaaagcc
    17221 agagtgatgg cggcatgatg ctgcgctttg tactgcgttc tgataccaag ctggcgcaac
    17281 tgcgtaaggc gctgccgtgg ttacacgaac aactaccgca gctgaaagtt attaccgtca
    17341 atattcagcc ggtacatatg gcgattatgg aaggggagac ggagatctac ctgaccgaac
    17401 aacaggcact ggcggagcgt tttaatgacg taccgctgtg gatccgtccg caaagtttct
    17461 tccagactaa tccggcggtc gccagccagt tgtacgccac cgcgcgcgac tgggtacgac
    17521 agctgccggt taaacatatg tgggatctgt tctgcggtgt ggggggcttt ggtttacact
    17581 gcgcgacgcc tgacatgcag ttaaccggga tcgaaattgc gccagaggcc attgcctgtg
    17641 caaagcagtc agccgctgaa ctgggcttaa ctcgtttaca atttcaggcg ctggactcca
    17701 ctcagtttgc cactgctcag ggggaggtgc cggagctggt gctggttaac ccgccgcgcc
    17761 gcggcattgg taaaccgctg tgtgattatc tttcaacgat ggcaccgcgt tttattatct
    17821 actccagctg taacgcccaa accatggcga aagatatccg cgaactgcca ggttaccgca
    17881 tcgaacgggt acagcttttt gatatgttcc cgcacaccgc gcactatgaa gtgttgacgc
    17941 tgctggtgaa gcaataaaaa agccacaggt gcggcttcag attgctgatc aggtgcgctt
    18001 tgttcatgcc ggatgcggcg tgaacgcctt atccggccta caaaatcatg ctaaatcaat
    18061 acattgcaga gttgatgtgg gccggataag cgcagcgcat caggcaatct tacttttgtc
    18121 atcagtctca attactgcgg aaaccactgg tcattgattt tctgatatgt accgtcagct
    18181 ttaattgctg ccagcgcgtt attcagtttt tccagcagag ctttgttatc cggacgtaca
    18241 gcgataccca ggccggtgcc gaagtattgc ggatcggtca ctttttcagt cgcaacgccc
    18301 agttgcggat tggttttcag ccattcgttt accaccgcgg tgtcaccaaa taccccatca
    18361 atacgaccat ttttcagatc gataaaggca ttctgataac tgtcatagga gacagttttc
    18421 acttccgggt gctgatcctg aatatatttc tggtgcgtcg taccgttttc catcccaatg
    18481 cgtttgcctt tcaggtcggc aaacgttttg taggtatctt ttttggaaat cacgacggct
    18541 gagttttcat agtagggtgt ggtaaacgaa acctgcttgc tacgctccgg ggtgatatcc
    18601 ataccggaga ttacggcgtc atattttctg aatttcagtg acgggatcag gctgtcgaac
    18661 gcgtgattag taaaagtaca ttctgcctgc atttgtttgc acaaggcttt tgccagatcg
    18721 atatcaaagc cgacaatctc attattagca cctatagatt caaagggtgg ataggtggct
    18781 gaaacgccaa aattgatttt ctctgcggca gaagcaccga aagtaaagga agcaagtaaa
    18841 gcggcaagaa ctaacttttt catgatggaa ctcccgtctg tcaatcttat gatttttggc
    18901 cgtgtctgcg gcatgggata acaatgccat caagtgaatt tatatgcaat aaacatgatt
    18961 aaataattta aatgaaataa aaaaagacgg ataacttagt gagttgtccg tcttcattat
    19021 aagaatttat gcactatgta ggccggataa ggcgtccacg ccgcatccgg cacaggcacc
    19081 gtgctgatgt ctgatgcgac gctggcgcgt cttatcagac ctacaaaccc ccccggcgaa
    19141 tgtacgcagc tacattaatt ccgccgttcg aatgccagtg ctttgcgctc gatcagacgc
    19201 atcatcagcg tcagcaggcc gttgacgacc aagtaaataa tccctgccgc accgaacacc
    19261 attacatcgt aggtgcgtcc gtacaacaac tggctgtatc ccatcacttc catcagcgta
    19321 atggtgtaag ccagagaggt acttttgaac accagcacca cttcgttgga ataagaagag
    19381 agcgagcgtt taaaggcata cggcagcagg atcgccagcg tatctttttt gctcattccc
    19441 agggcgctac aggactgcca ctgaccttct gggatcgcac gaattgcacc gtaaaacagc
    19501 tgcgtggtat acgccgcact gttcagcgat aacgcaatca gcgcacataa ccacggttct
    19561 gacaacaaat gccacagtgc cggatactcc tgcaacgtcg gaaactggcc cggcccgtaa
    19621 taaatcagga agatctgcac cagcagcggc gtaccggtaa acagcgtgat ataaccccgc
    19681 accagccaca ccagcaccgg cgttttcagc gtcaggatga tggtaaaaat caatgccaga
    19741 atcagtgcca caatcagcga ggcaacggtt agcgtcaggc tggtgtgcag ccctttcatc
    19801 agctcgggta aatactcaaa cattagctgg gcctccgctc aaaacgtgtc gcgcgcaggt
    19861 caatgcgttt gagaatgtac tgactgagca gggtgatcac caggtaaatc gccgccgcca
    19921 caatgtacca ggtaaatggt tcctgggtac gagtagcaat gcttttggtt tgtaacatta
    19981 aatcattcac actaatcaaa ctgaccagcg cggtatcttt cagcagcacc agccactggt
    20041 taccgaggcc aggcagcgca tgacgccaca tctgcggcat caccagacgg aaaaagatag
    20101 ccgatttcga cagccccagc gcctgaccag attcccactg gcccaccggt acagctttca
    20161 acgcgccgcg cagcgtttgc gaggcatagg cggcatacag cagtgacaga gcgatgacac
    20221 cacaaaggaa cggactcacg tcgaagttct caatgtccat ctgcaccggg atctgcacga
    20281 acccaagatt gatagtgaag ccatccgaaa gcgtcagcag taactgcgag gagccaaaat
    20341 agataaacag cactaccaga atttctggca ggccacgcag aatggttacc agcgctgaac
    20401 ctgcccacgc gacaggacgc catttcgctg actcccatac cgcaaagaac atcgccagcg
    20461 ccagcccgac aatcaatgca caaacggcaa ggccgacggt catcccggcg gcgcttgcta
    20521 aaggaaaaaa ttcattcatc aggaattact tctggaacca tttgttgtag atggtttcgt
    20581 aagtgccatc tttcttcact ttttccagcg cagtgttgag tttctgctgc agttcagtgt
    20641 ttccctgacg taccgcgatg ccgaggccag taccgaaata atctttatcg gtcactttat
    20701 cgcccactgc cgccagtttt gggttatctt tcagccactc agtgaccact gcggtgtcac
    20761 caaatacgct gtcgatacgc ccgttttgca gatccagttt tgcgttctgg tagctgtcat
    20821 acggaacggt ggtgatttcc gggtgcttat ccataatgaa cttctggtgt gtcgtcccgt
    20881 tctgaacgcc gacttttttg cctttcagct gatcaacact ggtgtatttg ccttgctgac
    20941 ccacaaacag ggcagagttg tcatagtacg gggtagtaaa cagtacctgc ttttcacgct
    21001 ccggagtgat atccatgccc gccatcactg cttctacgcg acggaatttc aggcttggga
    21061 tcaggctgtc aaacgcctgg ttagagaaag tacaggttgc atcaatctct ttacacagtg
    21121 cctgcgccag gtcgacgtca aaaccaacga tctggttgtt tgcatcaatc gattcaaacg
    21181 gaggataggg ggcttcggta gcaaaacgaa tggtttcggc agctgtggcg gaaagactaa
    21241 aacctgcaat taacgcggca atcagaactt ttttcattgt tgttatcccg aatcttagtg
    21301 agagagatag tttttaaatg cttcggtttg cggctcggta aagcagctcg cgtcgccttg
    21361 ttctacgata tgaccatttt ccatatacac cactcggctg gcggttttac gcgccacttc
    21421 aacttcgtgg gtgacgatca cctgggtaat attcgtttct gccagctcac gaatgatgct
    21481 gacgatttgt gccgtaattt ccgggtccag tgcggcggtc ggttcatcga acagcagtac
    21541 ctgcggttcc atcatcaacg cacgggcaat agcaacacgc tgctgctgac caccagaaag
    21601 atgcagcggg taacgatcgc tataaggttt gagacgcaga cgttccagca gtttttctgc
    21661 acgggccagc gcctgatctt tactcaaccc cagtacacgg cagggcgctt caatcaggtt
    21721 ttgctgcacg gtcagatgcg gccacaggtt gtattgctga aacaccatgc caacgttacg
    21781 acgcaaatcg cgaatcgctt tgtcagaggg tgttttggtg aaatcgaaat ggttgcctgc
    21841 aatgttgagc gtaccggagc gcggcatctc aagcagattg agtacacgca gcagcgagct
    21901 tttacccgcg ccgctggggc caagtaacac cagcgtttcg ccctgtgggc aatccagcgt
    21961 gatatcgaac agcgcctgat gcgcgccgta gaagcaatta atgccgttta attgaatact
    22021 cattgacact cgtatactgg cagtctgata gctattgagg tcgaagatag tacctttaac
    22081 agaataatta tgcaatattt atgctttaaa agttaaaagc aaagcgcatt attcaataaa
    22141 catagcacaa aataacgggg gcggtggtcg gtgagcataa atgtcggcat tcctcacgaa
    22201 atgccggata atttacgggg tttattggtt gatcaaggcg ttagcgattc tcgatggact
    22261 gacggagcgt acccgccgtg gcatgaacgc taccgcctaa gtagcgcaca tcgtcgatga
    22321 cccaacactg gccttcctga atcattaaca cttcatcctg ccagccctgg tcaccctgtt
    22381 tgagatccac ccgcaatgga atgttacggg catcacgatt agggatagtc gatgcactgg
    22441 caacgtgggc gctgtctggc aaggtggttc gactggagaa cggatcgctg cttaacaatt
    22501 cacgatgact attatcgcgg ctggcatcgc taagttgggt tgccagtttg tcactcaaat
    22561 aggggcgcag ggcggtaatg tcattgctgc gatgttgaat gcggtagtcg tagaattgtt
    22621 gagccacact gtcaggccct ccctggacac aggaaccggt gcgggggccg ttatctttat
    22681 aagctggcgt gacggtggta caggcactca gcagcagtgc acatggaata agtagagata
    22741 gtgaacggta gcgcataatc atttccttat tagcttgctg tctgcgtcac actacgacca
    22801 aacgctatca gattatttaa tacattgttt ttaatacaat tctgaaacaa tctgaacacc
    22861 tgattgggtt tgagtccatt gctggcgacc cactcaaggt tgctcgtagc tgacttactt
    22921 aaattaccca aacaatagac agtgtaaacc atttgtgagg ttgagcgtgt tatatggctg
    22981 aaatgatcat ttaaggtaaa tatgatagtt ctgaaatggt ggtataaatc ctcctgtaga
    23041 ggttaagagc atctgcaaga agccagtttc acatttataa cgcgtgataa atgaatgcgg
    23101 ttattgtcta atcattacaa aatttttcac atgcgataag agatcctgcg attaagatga
    23161 ttgccaaaat tacgttgtat ttttgaaaat gagcgaaatt tgatagtccc agctttgact
    23221 ctcgataata ttatttatgc tacaattgtg ggtaaagaat ttaagctaat gaatttgagt
    23281 gaagaataaa ttaaattgct tcagtatttc gttaattaat ataaatacaa aaggcgttgt
    23341 gcttttataa ttataaagcg cgaggagttc ggtggattat cgtttttctt gggtgatttt
    23401 taatatgata aacaatatat ctgatcaagc atcatcattt ccaggcacac aactaaatca
    23461 aagtgataat ttcctggatt cactgaggga atttttcgcg atattaaatc ccagtcgtaa
    23521 gggtgagttg tctacatggg ataccatcta cttgcatctg atattagcaa taaatgctga
    23581 cagtgatctc ataaaaaatg atgtgttgct cgcggaaaat attccatcag cgaattacca
    23641 atttaataca ttttttagca atactttcga gattgatgtt aaaaaatatc tgggtaaatc
    23701 agaagataac gaagttgaaa taaaagcggg taatgaaaga atctctattg gaatacgtaa
    23761 tgtatcgaat ggaaaacttg aaagacaaca gtttcttttc ccactggact atgagaataa
    23821 attacaagaa caattagata agtattttac gattgaatcg cacccattat tatacaggta
    23881 taccattggt agtaagattg cgaatgtgat ttttgaaaag ttatacagtc gtattgactt
    23941 taacaaagaa caatatatat cctttataaa ggacgcattt attcattttt atgattatag
    24001 caggcgatat gctattagtg agaatatcga taaagacgct gtaacaaaca atatcgcatt
    24061 gatgagcact ttttatgaca gtgataatac gtcaggtgag gttttaaata atgattttac
    24121 agaagaagag tcatttgaaa ctgcgttaga tgtagagcat gcaatagtgc ttggatttgc
    24181 tgatgataat tttgaaacaa aacctgttca ttatcaggat ttattaacac gatttagtgc
    24241 ctttcaagat accgtgttta atctgtttcc tgaaatgcac tcttcgcatt accacgatat
    24301 ttgtagcgta agtgtggata tgacaaaggg aacgcaatgc atgattcact tgatggtgaa
    24361 tgaagaagtt ttcatgtctc tgcctgttcc agttgccacg atggtacgtg aagatgcaag
    24421 taatttagtc aatcttaaaa cactactcaa tgatggttgt ttcattaaat atagtcattt
    24481 taatgacgtg gcattaataa agcaaaacat aagtaatctc tatttatcac acactgttgt
    24541 gaatgaaagc atattgaaaa aatgttgttt tgaaaacggc agtttaggtg atgttaaaat
    24601 aacaaatagc aatgtaataa acagtgcatt caaaaatatt tcgtttagat ctgttaaaat
    24661 taataacgta aatactcatt ctttaaaatt tattaactgc aattttttca atgttgatat
    24721 gataagagtt aatctcagca aatgtctttt tcatgaatgt agtatgcatg gtgtcaagat
    24781 aaaaccctgg ttgcctgtaa aatggacaaa agaattaata agtgattatt tgtatggctg
    24841 tttactatct ctgtactcta tctgtgcaag ggatatatat aatatgaacg ccgggaataa
    24901 cgtgaaggtt gctgcggatg ctttccttga aattattttc tctcttaaaa ataaatattg
    24961 tattaaacta ttaagcgctc aggatcgggc gtttatatac gagtttgcaa gaatgatctt
    25021 cgcttatatt aatgacaaaa gcattgagat cttattactt tcttgttttg cggcggctga
    25081 ccaaaaagca atccagcggt atagacctca gtcacaagat ggggaagact ttaggagtca
    25141 tttacaatat aagttgcctc tttccgctca ttaatgttgt tggtgcaaca taataagaca
    25201 ggtaagcggt accctataat gaaaccaata tcgggttatg caacggataa tcgtaaagac
    25261 ttaagtgggt gtttttgaca caatgagatg atttcctaaa ctgatcaaaa ccttcttccc
    25321 aacatggcat atcagtccga taatgcttta gagatcgaag gcttaacaaa caaggagatc
    25381 gatcatgcaa ttttctacaa ccccaactct ggaaggcctg accatcgttg aatattgcgg
    25441 tgtggtgacc ggcgaagcga ttttaggtgc caatattttc cgtgatttct ttgccggtat
    25501 ccgcgatatc gttggcggac gttccggtgc gtatgaaaaa gaactgcgta aagcacggga
    25561 gatcgccttt gaggaattag gctcccaggc gcgggcgctg ggggccgatg ccgtagttgg
    25621 tattgatatc gactacgaaa cggtcgggca aaacggcagt atgctgatgg ttagcgtcag
    25681 cggtacggcg gtgaaaacgc gtcgatgaga agagtcttct ggctggtcgc tgccgctctg
    25741 ttactggcag ggtgtgcagg cgaaaaaggc attgtcgaga aagagggata tcagcttgat
    25801 acccgacacc aggcgcaggc ggcgtatccg cgcattaaag tgctggtgat ccactacacc
    25861 gcagatgatt ttgatagctc gctggcgaca ctgactgata agcaggtcag ctcgcattat
    25921 ctgatccctg cggtgccacc gcgatacaac ggtaaaccgc gcatctggca actggtgccg
    25981 gaacaagaac tggcctggca tgcggggatt agcgcctggc gcggggcaac gcgccttaac
    26041 gacacctcta ttggcattga gctggaaaac cgtggctggc aaaaatcatc cggagtgaaa
    26101 tattttgccc cgtttgaaca ggcacagatt caggcgctta ttccgctggc gaaagatatt
    26161 attgcccgtt atcacatcaa gccggaaaac gtagtggcac atgcggatat cgcaccgcag
    26221 cgcaaagacg atccggggcc attatttccc tggcagcaac tggcgcagca ggggattggt
    26281 gcctggccgg atgcgcagcg ggtgaacttt taccttgccg ggcgcgcgcc gcacacaccg
    26341 gtagataccg cgtcattgct ggagcttttg gcgcgctacg gttatgacgt aaaacctgat
    26401 atgacaccgc gcgagcagcg gcgcgtgatt atggcattcc agatgcattt ccgcccgacg
    26461 ttatataacg gcgaagcgga tgcagaaact caggcgattg ccgaagcatt gctggagaaa
    26521 tacgggcagg attagcgcgg cagttttccg tggtcgcgta gccaggcggc agttttctcg
    26581 atgccttcat ccagggtgat gaccggctga taacctaact cttcctgcgc acgcgtaata
    26641 tccagcgtaa agtcaaaatt caacttggag gcgccgtagt gggtcagcgg cggctctttt
    26701 gctgacttgc ggcctaaacg ctccatgctg cgggcgatca tatccagcat cgggtaaggg
    26761 acggaacgaa tacgacagtc aatattcaac tcgtcgatca gcttctgcac gatgctgcgc
    26821 agtgtgcgat gctcgccgtt ggtgatgttg tacacacgcc cggaaggtag cttatcgcag
    26881 gcttcctggc ttgccagcca cattgcgtgc acggcatttt catagtaggt catatcgacc
    26941 agcgcactgc cgccatgcgg taacagaata ctgccgtagt ggtgcatcat atgcgccaga
    27001 cggggaataa agactttatc gtgcggtccg aacagacttt gtgggcgcag aatagtaaag
    27061 cgcgtttgtg gattcgcctg cgaaagcata ttgatcactt cttcgctggc tgctttgctg
    27121 cgggcaaact cgttggcgaa gcggtgaggg cgaaaatctt ctttaatatt gcgatggtgg
    27181 tgataatcga agtacaggga gggggaagag atatgaataa agttacgcac accccaggcg
    27241 acagcccatt cacccaggcg gcgagtggcg cgaacgttag ccagatcgaa agcctgttgt
    27301 gtcccccagg gtgaggtaaa gctggagcag tgccacagcg tatcaatgcc cgcgagcatc
    27361 actttagctt gtgatgaaac cagctcggtc agatccgtcg gaacaaactc tgcgcccatt
    27421 ttttccagca atttacccat tgcttcgttg cgaccgctcg cacgcacgct gatgcctttc
    27481 tggcataaaa actctaccgc gtttcgacct aagccgctgg tggcgccggt aaccagtacc
    27541 ttcatatcaa tccactgttg ttgagaaaat gacgtgcgca ttcttccgtg atttccccca
    27601 tgatgcaatg ggaaacatga aagaataacg caggttttgt cgattaatct gtgctttgtt
    27661 ctgccagtct ggcgatttgt tttgccattc cgcgaaaaat aaacagatgc gcggggatca
    27721 tcaataacca gtaaaacagc cccggcatac cgtgcggatg ccagaaagcg cggacatcga
    27781 tagtacgata gtcaccttta tcttccaggg taaaacacag tcgtcccagc cccggcgctt
    27841 tcatgccaaa taacaacgta agttgctttt tcggttcaac gacaatcact ttccagctat
    27901 ccaccgcatc gccggtctgt aaatattcgc gctccgggcg gcctttcgcc agattatgac
    27961 cgatcgcgcg gtccatcaac gcccgtgtct gccacaaaat attgccaaag aaataacgct
    28021 ctttaccgcc gatttggttc accaccagcc ataaagccgc aaggctggcg gacgttttaa
    28081 cggtaaaccc cgcctgtttg gcgaaataac cgtactccgg tcgccagcgg gcaaaggcct
    28141 gagcgtcgta gccccagtcg ctggagttga ccagtttttc ttcctctttc aacgtgctac
    28201 gtaccgcgtc atcgaaagcg atcagccgtt gtgggatgag tgcacgtagc gcggtatcat
    28261 ctgccagcag atcgtgtttc agcccctgaa tcaacgccct ggcggtggtg ggcggtacgg
    28321 aagtaatcac attgagaaac cacaccgaaa tccagcgggt ggggaggggg atggggatca
    28381 accagcggcg cttaccgctc accgccataa aatgttcaaa ctgttgctga taactgagca
    28441 cctctggtcc ggcggcttcg aagatgcggt gttcgttggc tggatggtct aacagcgcca
    28501 ccagatagtg cagcaagttt tccagcgcga tgggcgtggt gcgtgaacgt acccagcgtg
    28561 gcggcgttaa cactggcagg ttgtagacca tatcgcgcat gacttcgaac gccgctgaac
    28621 ctgcgccaac gataattccg gcccgaagtt cggtcactgg tacattcgct tcacgaagaa
    28681 tgtccgccgt agcctgacga gctcgcagat gatccgactg ctcatgtggc ggggcctgca
    28741 acgaactgag aaagattaat tgcttaactg gtacttcacg tagcgcatcg cggacgttga
    28801 gagccagctg gcgctcctga gcgataaaat cgccgccttc gcccatgctg tgcaccagaa
    28861 aatagacggt atcgatatcc tgcaacaggg ccggaaggtt atccggccag ctgagatcga
    28921 ctttatggca actgacgttt gccagttgca gctttgcaag cctgtcgaca tgacgtgccg
    28981 ccgctaggat ctgatgccct tgctggctga gtgtgcgcac cagatgctga ccaatgtagc
    29041 cactggcacc gagaactaaa atgcgttgcg gcacgtctct ctccttaacg cgccaggaat
    29101 gcgcgccagt gggcggcgac ttccgccagt tgttcgcgcg agacgtcaag atgcgtcacc
    29161 aggcggacaa tcggcgaggc gttaatcagc acgtttctcg ctttcatgta ttcgcctaac
    29221 gcggcagcat tttcttcccc gacgcgaaca aacagcatat tagtgtcctg acgcatcaca
    29281 tccgcgcctg cttcacgcag ttgctccgcc atccaggcgg cgttgtcgtg atcttcctgc
    29341 aaccgtgcga cgttattttc cagggcatac atcccggcgg cagccagaat cccggactga
    29401 cgcatcccgc caccggccat tttccgccag cggatggcac gtttaatgta atcacgatta
    29461 ccgacgagta atgaaccgac tggcgtccca agacctttcg acaggcaaat ggtgaacgaa
    29521 tcacaatatt gcgtgatctc tttcagttcg cagccgtaag ccaccacggc attaaagatg
    29581 cgcgcaccgt caacatgcag cgccagattg cgctcgcggg taaattccca tgcttctttc
    29641 aggtattcac gcggcagcac tttgccgttg tgggtgtttt ccagactgag taatttggtg
    29701 cgggcgaaat ggatatcgtc gggtttgatt ttcatcgcca ctttatccag cggtagcgtg
    29761 ccgtcggcag ccgcgtcgat gggttgcggc tgaatactgc ccagcaccgc tgcgccaccg
    29821 gcttcaaaca gatagttatg cgcggcctga ccgacaatat actcttcgcc acgttcgcag
    29881 tgactgagca gagcgaccag gttggcctga gtgccggtag gcagaaaaat ggcggcttct
    29941 ttaccggaaa gctctgctgc atagtcctgc agagcattaa cggtagggtc gtctccgtaa
    30001 acgtcgtccc caaccggggc ggccatcatc gcttcgagca tggcgcggct cggtcgggta
    30061 acggtatcac tgcgtaaatc aatcatggca tgtccttatt atgacgggaa atgccaccct
    30121 ttttacctta gccagttcgt tttcgccagt tcgatcactt catcgccgcg tccgctgata
    30181 attgcgcgca acatatacag gctaaaacct ttggcctgtt cgagtttgat ctgcggtgga
    30241 atggctaatt cttctttggc gaccaccaca tccaccaaca ccggaccgtc gatagagaag
    30301 gcgcgttgca gggcttcatc aacttcagag gctttttcta cacggatacc cgtaatgccg
    30361 cacgcttcgg caatgcgggc aaagttggtg tcgtgcagct cagtaccgtc tgtcaggtaa
    30421 cctccggcct tcatctccat cgccacaaag cccagcacgc tgttattaaa gacgataatt
    30481 ttcaccggca gtttcatctg cactactgag aggaaatcgc ccatcaacat gctaaaaccg
    30541 ccatcgccgc acatggcgac cacctgacgt tccggctcgg ttgcctgcgc acccaacgcc
    30601 tgcggcatgg cgttagccat cgaaccgtgg ttaaacgaac ctaacaggcg acgcttgccg
    30661 ttcattttca gataacgtgc cgcccatacc gttggcgtgc cgacgtcaca ggtaaagatg
    30721 gcgtcatcgg cggcaaaatg actaatttgc tgcgccagat attgcgggtg aatggctttc
    30781 tcgctcggtt tagctaaatc gtccagccct ttgcgggcat cgcggtaatc ttccagcgct
    30841 ttatccagaa acttgcgatc ggttttttct tccaccagtg gcagcaatgc gcgcagggtt
    30901 gatttgatat cgccgaccag cgccatatct accttgctat gcgcgccgat gctggctgga
    30961 ttaatatcaa tctgaataat tttggcatcg gtcgggtaga acgcacggta ggggaactga
    31021 gtaccgagta gcactaacgt gtcggcgttc atcatggtat ggaaacctga cgagaatccg
    31081 attaacccgg tcattccaac atcatacgga ttatcgtatt cgacatgctc tttaccgcgt
    31141 agggcatgaa caataggtgc tttaattttc ccggcaaact caactaactc tttatgcgcc
    31201 cccgcgcagc cgctgccaca catcagggcg atattgctgg aataacgcag cagttgcgcc
    31261 agtttgcgta actcttcttc ttccggcatc acgactggct gtggcgcatg ataccagtgc
    31321 gtggttgccc cttctggcgc aggttttaac gccacgtcgc ccggtaatac aacaacggaa
    31381 acgccacggt taagcaccgc tttgcgcata gcaattgcca gcacttgtgg gatctgctcc
    31441 gggctggaaa ccagctcgca atagtgacta cattcgcgga atagctcttg tgggtgggtt
    31501 tcctggaaat agccgctgcc aatttcgctg gagggaatat gagcggcaat cgccagtacc
    31561 ggaacgttat tgcggtggca atcgaatagg ccgttgatca agtgcaggtt gcctgggccg
    31621 cacgatccgg cacagaccgc cagttctcct gaaagttgtg cttcagcgcc agcggcaaac
    31681 gccgccactt cttcatggcg ggtggacatc cactcgatgg tgcccatgcg attaagactg
    31741 tcgctaagac cgttcagaga gtcgcccgtg actccccaga tgcgtttcac gcctgccgat
    31801 tcgagtgttt tggcgatata agctgcaacc gtttgtttca tggttctcca tctcctgaat
    31861 gtgataacgg taacaagttt agttcatctg acggaggggg aagggatggg agagaaagga
    31921 ggcactaacg gttaaatagc ccgatgaaag gaatatcatc gggcataagg cgattatgcg
    31981 agaaccaaat ccccctgcgg atggcaggag caggccagta cgtaaccttc agcgatttcg
    32041 gcgtcggtca gcgtcattgt actgctcacc gtatattcac cggaaaccac ttttgtctta
    32101 cagcagccgc aaacacccgc acggcaggca gcgacaaccg gaacgttatt gctttccagc
    32161 gcctccagta gcgtggtgcc aaccggggcg taaaattctc gtgccggttg cagtttggtg
    32221 aatttcagac cgctggtcgc cgcttctgct actggggtga agaatttctc tttaaagaaa
    32281 cgcgtcacgc cgagcgcttt cacttcctgc tctacccaat ccatatacgg agccgggccg
    32341 caggtcatca cggtacgtga agctaagtca ggtacatctg ccagcagttc gcgagtgagg
    32401 cgaccagcga taaagccttc ggtaacgtta ttttctgcca ccagcgttac cggatagtta
    32461 cgccactcat cggcgaaaat aacatcctgc ggcgtacgca cgttgtagat cacccgcaca
    32521 tcggcctgtg gacggttctt cgcaagccag cgacgcatcg acataatcgg cgtgacgccg
    32581 cagcctgccg ccagcaacag gaatttatct tctgctttat cgtcgcaggt aaattccccc
    32641 atcgcgtccg aaagccagag ataatcaccg cgttttacat cgcgcgtcag ccactgggag
    32701 ccgacaccgt catcaatccg ccgcacggtc agggtgatat attcactcac gcctggcgtg
    32761 gaggaaatgg tgtaagcacg cagcgtttcc gctgagtttc gcacgctgac cagtgcatat
    32821 tgcccggcgc gatatgggta gtgatcgtgg caaatcaggg aaatcgtcca cacatccggc
    32881 gtttcttgcg taatgtgatg aacctgtatc cgccacgggc attgattcgt tggcatcgtc
    32941 atcgacaaac tccttacgcg ttcaacagtt gcttcatgtc ttcttcaaca gtggtgatag
    33001 aacgcaggcc gaatttctcg ttcagcaccg ccagcaggtc tggtgtcagg aaaccaggtg
    33061 cagtcgggcc ggtgacgata tttttcacgc ccagagaaag cagcgtcagc agaatgacaa
    33121 tcgctttctg ttcaaaccag gagagcacca gcgacagcgg cagatcgttc acaccacagc
    33181 ccagtttctc tgccagagtg acagccagaa taattgctga gtaagcatcg ttacattgac
    33241 ctgcatctac cagacgcggc agaccttcga tatcgccaaa ctcaagtttg ttaaagcgat
    33301 atttaccaca ggcgagggtc aggatcaggc agtcatccgg cacgctggtg gcgaaatcgg
    33361 tgaagtagtg gcgctcgccg cgtgcgccgt cacagccacc aagcaggaag atatgacgca
    33421 gtttttcacg gctaaccaga tcaatcagcg tatcggctgc accaagcagc gtctggcgac
    33481 caaagcccac ggtgatcagg tgaggaattt cgctgtacgg gaagcccgcc atttgttgcg
    33541 cctgggcgat gaccgcagag aaatcttcgc catccagatg acgcacgcca ggccagccaa
    33601 cgatgctgcg ggtccagata cgatcgtcat aagcgcctac ggttgggtcg atgatgcagt
    33661 tcgaggtcat cacgatgggg ccagggaaac gagcgaactc cacttgctga ttctgccagc
    33721 cgctgccgta gttaccgacc agatgcttga atttacgcag ctccgggtag ccatgcgcag
    33781 gcagcatttc gccgtgggtg tagacattaa cgcccgtgcc ttcggtctgt tccagcaggt
    33841 tgtagagatc tttgagatcg tgaccggaaa tcagaatgca tttacccgcc gtcgctttga
    33901 cgttgacctg ggttggcgtc gggtgaccgt atttaccggt ttcgcctgca tccagaatgc
    33961 tcatcacttt gaagttcatc tggccgattt ccattgaaca ctcaagaagc gcgttcatat
    34021 cggcaggcca ggtccccagc cacgccatga ttttatggta ctgggcataa atatcgttgt
    34081 cgtattgacc gagaacatgc gcgtgttcca tataggccgc cgcacctttc aggccataca
    34141 ggcacagcag acgcaggccg agaatgtttt cgccaatcgc cgctttatct ttgttagggg
    34201 taaattctgc tgcctgacgt tgcagctcgc cgagatcatc gctcaccagt tgcaggtcag
    34261 ccatcgggtt atcgacgcgc gcgttggcat ctacagccag gcattgtgct ttcagcgcct
    34321 cgcgcaaggc aatcgcttca cgagcgtagc cgacaatacg cggagaatcg aagttaacgt
    34381 tggtcagggt tgagaaaaag gcacgtggcg cgaagctgtc aacatcgtgg ttgatgatgc
    34441 cgtattcacg cgcttttacc gcccaggcag aaagcccttg cagcgccgcg atgagtaaat
    34501 cctgaaggtc agaagtttcc gccgttttac cacacatccc ctgcgcgtat gagcagccgt
    34561 tttctgccgg agtacggata gtttgttcac attgcacaca aaacatgatc acacctttta
    34621 aagttatatt taatatacat gtttaagggt aagacgctta acgcggggat aaaagggatt
    34681 tttcatgcaa ctttaaggga gattgattta gcgcaatttt ggcggcaggg aactaccgcc
    34741 agagaggtat tacgcagaga aaaaggcgat gaggatcggc actaacaggc taagaataaa
    34801 accgtgaaca attgccgccg ggaccatatc cagcccgcca gtacgttgaa gaacgggcag
    34861 ggtgaaatcc attgatgtgg caccgcataa gcccagtgca gtagagcggc tgcggcgaat
    34921 cagcccaggg atcaacataa tagcaatcag ttcacgggcc agatcattaa aaaacgccgc
    34981 gctcccgatt accggaccaa aagattcggt caataaaata ccggaaagag aataccagcc
    35041 gaaaccggag gccattgcca gcgcggtatt gatggggaga tcaagaataa aggcgttaat
    35101 taaaccacca attaatgaac tgacaaccac caccacggcg acaatcattc cccggcgatt
    35161 aaggacaatc tgctttaagg tcatgccatt attgcgcaac tgaataccaa cgaggaaaag
    35221 tagcaaaatt aacgtgtatt cactggcttc ggtcgcgtgt tgtaagaaag ccagtccact
    35281 cagaccaatg gcaaaaccaa tcactactac gccgcacagt tttagcgact ccagcgccat
    35341 cgcaatacgc gacgggagtt tttcttgctg atggtggttg cgccacggca ggcctcgctc
    35401 cagccacatc agggcggcaa tattacacag taaaataacg gtaatactga cggcagaata
    35461 atgcagaatc gccaacaggt tactggcgag gttatcgaga aacgccagac tgatacccat
    35521 aaaaaagaga ataaggtaaa ccatccagct taatagctga ttaatagctt ttaacgcagc
    35581 ttgttggcga agcggaatga ggtaacccac aatcagggga accagaatga ttaacagccc
    35641 agaaaacatg aaaatccagt ccttgcaaag aagaagtcga aatgcgcgat gacacactac
    35701 tgaaagcgga agggcgagta aagttgcaat taaaaggaaa tgttatgcat cagag
//
LOCUS       DAJTSI010000023        35195 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000023 DAJTSI010000000
VERSION     DAJTSI010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35195
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.023"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            143..750
                     /locus_tag="OO327_001050"
                     /pseudo
     CDS             143..750
                     /locus_tag="OO327_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(777..1682)
                     /gene="yijE"
                     /locus_tag="OO327_001051"
     CDS             complement(777..1682)
                     /gene="yijE"
                     /locus_tag="OO327_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR6017680.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(1776..3956)
                     /gene="katG"
                     /locus_tag="OO327_001052"
     CDS             complement(1776..3956)
                     /gene="katG"
                     /locus_tag="OO327_001052"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR6017681.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPIQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(4285..5175)
                     /gene="metF"
                     /locus_tag="OO327_001053"
     CDS             complement(4285..5175)
                     /gene="metF"
                     /locus_tag="OO327_001053"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR6017682.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(5524..7956)
                     /gene="metL"
                     /locus_tag="OO327_001054"
     CDS             complement(5524..7956)
                     /gene="metL"
                     /locus_tag="OO327_001054"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR6017683.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(7959..9119)
                     /gene="metB"
                     /locus_tag="OO327_001055"
     CDS             complement(7959..9119)
                     /gene="metB"
                     /locus_tag="OO327_001055"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR6017684.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            9396..9713
                     /gene="metJ"
                     /locus_tag="OO327_001056"
     CDS             9396..9713
                     /gene="metJ"
                     /locus_tag="OO327_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR6017685.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            9897..10505
                     /locus_tag="OO327_001057"
     CDS             9897..10505
                     /locus_tag="OO327_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR6017686.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(10566..10778)
                     /gene="rpmE"
                     /locus_tag="OO327_001058"
     CDS             complement(10566..10778)
                     /gene="rpmE"
                     /locus_tag="OO327_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR6017687.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            10981..13179
                     /gene="priA"
                     /locus_tag="OO327_001059"
     CDS             10981..13179
                     /gene="priA"
                     /locus_tag="OO327_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR6017688.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            13335..14360
                     /gene="cytR"
                     /locus_tag="OO327_001060"
     CDS             13335..14360
                     /gene="cytR"
                     /locus_tag="OO327_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR6017689.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            14452..15411
                     /gene="ftsN"
                     /locus_tag="OO327_001061"
     CDS             14452..15411
                     /gene="ftsN"
                     /locus_tag="OO327_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR6017690.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            15504..16034
                     /gene="hslV"
                     /locus_tag="OO327_001062"
     CDS             15504..16034
                     /gene="hslV"
                     /locus_tag="OO327_001062"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR6017691.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            16044..17375
                     /gene="hslU"
                     /locus_tag="OO327_001063"
     CDS             16044..17375
                     /gene="hslU"
                     /locus_tag="OO327_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR6017692.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            17442..18368
                     /gene="menA"
                     /locus_tag="OO327_001064"
     CDS             17442..18368
                     /gene="menA"
                     /locus_tag="OO327_001064"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR6017693.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            18461..18946
                     /gene="rraA"
                     /locus_tag="OO327_001065"
     CDS             18461..18946
                     /gene="rraA"
                     /locus_tag="OO327_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR6017694.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(19031..19276)
                     /gene="zapB"
                     /locus_tag="OO327_001066"
     CDS             complement(19031..19276)
                     /gene="zapB"
                     /locus_tag="OO327_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR6017695.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            19702..20547
                     /gene="glpF"
                     /locus_tag="OO327_001067"
                     /pseudo
     CDS             19702..20547
                     /gene="glpF"
                     /locus_tag="OO327_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            20587..22077
                     /gene="glpK"
                     /locus_tag="OO327_001068"
     CDS             20587..22077
                     /gene="glpK"
                     /locus_tag="OO327_001068"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR6017696.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            22307..23317
                     /gene="glpX"
                     /locus_tag="OO327_001069"
     CDS             22307..23317
                     /gene="glpX"
                     /locus_tag="OO327_001069"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR6017697.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            23414..24160
                     /gene="fpr"
                     /locus_tag="OO327_001070"
     CDS             23414..24160
                     /gene="fpr"
                     /locus_tag="OO327_001070"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR6017698.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(24165..24593)
                     /gene="uspD"
                     /locus_tag="OO327_001071"
     CDS             complement(24165..24593)
                     /gene="uspD"
                     /locus_tag="OO327_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR6017699.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(24620..24919)
                     /locus_tag="OO327_001072"
     CDS             complement(24620..24919)
                     /locus_tag="OO327_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR6017700.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(25131..25571)
                     /locus_tag="OO327_001073"
                     /pseudo
     CDS             complement(25131..25571)
                     /locus_tag="OO327_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            25672..26271
                     /locus_tag="OO327_001074"
     CDS             25672..26271
                     /locus_tag="OO327_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR6017701.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            26379..27146
                     /gene="tpiA"
                     /locus_tag="OO327_001075"
     CDS             26379..27146
                     /gene="tpiA"
                     /locus_tag="OO327_001075"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR6017702.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(27201..27956)
                     /gene="cdh"
                     /locus_tag="OO327_001076"
     CDS             complement(27201..27956)
                     /gene="cdh"
                     /locus_tag="OO327_001076"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR6017703.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGHTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(28063..29052)
                     /gene="sbp"
                     /locus_tag="OO327_001077"
     CDS             complement(28063..29052)
                     /gene="sbp"
                     /locus_tag="OO327_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR6017704.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(29372..30334)
                     /gene="pfkA"
                     /locus_tag="OO327_001078"
     CDS             complement(29372..30334)
                     /gene="pfkA"
                     /locus_tag="OO327_001078"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR6017705.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(30515..31417)
                     /gene="fieF"
                     /locus_tag="OO327_001079"
     CDS             complement(30515..31417)
                     /gene="fieF"
                     /locus_tag="OO327_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR6017706.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(31565..32065)
                     /gene="cpxP"
                     /locus_tag="OO327_001080"
     CDS             complement(31565..32065)
                     /gene="cpxP"
                     /locus_tag="OO327_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR6017707.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            32215..32913
                     /gene="cpxR"
                     /locus_tag="OO327_001081"
     CDS             32215..32913
                     /gene="cpxR"
                     /locus_tag="OO327_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR6017708.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            32910..34283
                     /gene="cpxA"
                     /locus_tag="OO327_001082"
     CDS             32910..34283
                     /gene="cpxA"
                     /locus_tag="OO327_001082"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR6017709.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(34389..35063)
                     /gene="yiiM"
                     /locus_tag="OO327_001083"
     CDS             complement(34389..35063)
                     /gene="yiiM"
                     /locus_tag="OO327_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR6017710.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT         8637 a         9372 c         9036 g         8150 t
ORIGIN      
        1 ccgcatccgg cactctgtgc cagtaatctg aaactcccac gttgttcagg agttttttat
       61 ccgcttctgg acattttctc cacagaacaa tcgttagccc ggaaggtcga atctgtgact
      121 cttattttca cggacgaacc agatgaaagc ttcactggcg ctgctcagcc tgctaacggc
      181 atttaccagc cactccttaa aatctcccgc cgttccacca accgtggtac aaatccaggc
      241 caataccaac cttgccatcg cagacggtgc cagacagcaa attggcagca cgctatttta
      301 cgatcgcagc ttacctatcc tggcggtgat gttccgcaag aacgcggtgt atgttccgat
      361 atggtgatcc gcgcattgcg cagccagaag gtcgatttgc agaaactggt tcacgaagat
      421 atggcgaaga attttgctga gtacccgcaa aaatggaagt taaagcgccc ggacagcaac
      481 atcgatcacc gtcgggtgcc taatctggaa acctggttta gccgccacaa caaaacacgc
      541 cccatcagta aaaatgccgg tgactatcaa gcgggcgata ttgtctcctg gcgattagat
      601 aacgggctgg cgcatattgg ggtggcgtca gatggcttcg cccgcgacgg tacgccgctg
      661 gtgatacata acattggcgc aggcgcgcag gaagaagatg tgctgtttag ctggcggatg
      721 gtggggcatt atcgttattt tgttaaataa gagatcgccc cacatgaaga aaaaattcag
      781 atgcttttta cactgacagc ttcttttttc ttacggctca ccagcgccag tgccagcaca
      841 atcagcacaa taccgctacc ttcaacggcc cccggattct caccgagcag ccaccaggaa
      901 aagagtacgc cacaaacggg aacggccagt gtgcttaagc tggcaatact ggcaggcaag
      961 tttttcagca caaacagcca taagctccac gctagcgccg tcgccagaat cgcactgtag
     1021 gccagcgccc agaacacggt gggctgccag tcaatttcac gttgcggcac cagtaaagcg
     1081 accacactca tcaccagcgc cgcatacagc atctgccagg atgttaacga taataaatcc
     1141 acgcgcggat gacgggcgta taggcgttta gcaacaatcg cgctcgcccc ccaactaaca
     1201 ccggagagga ttgccagcat ggcacttttc atcgaagaga aatcgagttg ccacggctgc
     1261 aacaccaaaa ataagccgaa agcggcaatc agaatcgcga aatattgccc acgtcgcagg
     1321 cgttcaccga gaaacaacgc ggcgaaaatc accacccaga acggcatggt atagctcagg
     1381 atcgccacct tccccgcacc tccgctgacc aacgcccact gcgccagacc aaccatcccg
     1441 caggtttgta acagggcaat ggctaaggtg tatttaaacg gcgtcgggcg cattccgcga
     1501 ccacgtaata aaaggacgat gaataaaacg agagcgccga aaatgcagcg taaggcggta
     1561 aagtcgaagg caccgatgta actggtgact tgcttcatga aaatccagct ataactccag
     1621 ataagtgtga gcacaaccaa gccactgatt gccagtgggt tgctctttcc tgcggcagac
     1681 atgagagatc cagtgtgtag ttttcctgtg gacactatac gccggtaaag aaacttcagc
     1741 gactgcctga aagacagtcg ctgaagaagg tcagattaca gcaggtcgaa acggtcgagg
     1801 ttcatcactt tcacccatgc cgctacgaag tctttaacaa acttctcgtg ggcatcgctg
     1861 ctggcgtaaa cttccgctac tgcacgcagg acagagttag aaccaaatac cagatccgca
     1921 cggctggctg tgtatttcac ttcgccggtt tcgcggtcac gtccttcgaa cagctctttc
     1981 gattcgtcag tcgctttcca ctcgtaacgc atatccagca agttcacgaa gaagtcattg
     2041 ctcaatacgc caacgcggtc agtgaagacg ccgtttttgc tgccatcgaa gttggcaccc
     2101 agtacacgca tcccgcccac cagcgcagtc atttccggcg cggtcagcgt cagttgctgt
     2161 gctttgtcga tcagcagtga ctcggtggtg gaaacgtcca gacgagcgcg atagttacgg
     2221 aaaccgtcag caattggctc cagcagctca aacatctcaa tgtcagtctg atcctgacgc
     2281 gcatcaacgc gacccggcgc aaacggtaca tgaatgctca aacctgcggc gcttgcggct
     2341 ttctcaacac caaccacacc agccagcacg atgatatccg ccagcgaggc tttaccagac
     2401 tctttctgga ttttctccag aacaggcaga gcacgaacgg ctgcggcgtt cacatcccag
     2461 tcgcgctgcg gcattaatgc cagacgcgca ccgttggcac caccgcgttt gtcgccacca
     2521 cggaaggtag aagcagatgc ccaggccacc gataccagct cactaacaga cagaccagaa
     2581 tccgcaatcg cgaatttcag atcgataatg tcctgctcgg tcgggttgta gatcggctgc
     2641 ggcagcggat cttgccagat cagatcttct ttcggcactt ccggcccgat gtagcgagat
     2701 ttcggcccca tatccctgtg cgtcagtttg aaccaggcac gggcaaaggc ttcgttgaac
     2761 gcctgcggat cgttgaggaa acgacgagag atcttctcga actcaggatc aaaacgcagc
     2821 gtcaggtcgg tcaccagcat tgtcggttta cgtttcttcg acggatcgaa cggatccggg
     2881 ataatttccg gtgcgtctac cgcttcgaac tggattgctc cagccggact gcgggtctgt
     2941 acccactcat acttgaacag attctcgaag aaatagttgc tccactggat cggcgtctgg
     3001 gtccagacta cttccagacc agaggtaatg gcatctgcgc caacgccgct gccgtaagtg
     3061 ctcgcccagc ctaaaccttg ttcttcaatc ggtgcagctt ctggatcagg acctacgttt
     3121 gatgtcggac cggcaccgtg ggttttaccc agcgtatgac cacccgcaat cagcgccacg
     3181 gtttcttcgt cgttcatgcc catgttgccg aaggtcgcgc ggatagctgc tgccgcagaa
     3241 agcggttcgc cgctgtgatc cgggccttcc gggttaacgt aaatcaggcc catctcggtt
     3301 gcacccagcg gtgctttcgc cagcgcttcc ggatgacggt gagtcagcca ggctttttca
     3361 tcaccccagt taacatccag atccggttcc cagacgtctt cacgaccggc accaaaaccg
     3421 aaggtacgga agccggagtt ttccagcgcc acgttacctg cgaggataaa caggtcggcc
     3481 caggagattt tctgaccata tttctgtttg attggccaca acaggcgacg cgctttatcg
     3541 aggcttacgt tatccggcca ggagttcagc ggtgcaaaac gttgctgacc acgacccgcg
     3601 ccgccgcgtc catcgattga acggtaagta cctgcgccgt gccaagccat acgaataaac
     3661 agaccggcgt agctgcccca gtcggctggc caccacggct gagattctgt cagcagggct
     3721 ttcagatctt ttttcaggcc gtagtaatct aatttgctga attctttgcg gtagtcaaag
     3781 tcctcaccca gcgggttaga acgattagaa tgttggttta acaggtcaac acggagttga
     3841 tttggccacc agtcgcgagt ggttgtgccc gcccccgcgc tctggtcgtg accgccctga
     3901 tggaacgggc atttgccagt ggctgtggtg ttatggatat cgtctgacgt gctcatcaat
     3961 gtgctcccct ctacagtgtt accgttacga tacacagcgt tagagagaag ttataattga
     4021 attaatccac ggatgcgata gttgagatct tacatattgt tggttaaaga gatgtagatc
     4081 aaattgatcg taattaagct tttttgatta tgtccgaatt cggacaaatg ctttataaaa
     4141 aagggtttta gcttcatatc cttcaagtaa tgagggagaa aacaaggctg acaaccttat
     4201 tttcccgccc tcatttcgag gcagcatttt gtgctctgtt taaaatttgt gatcactgtg
     4261 tgattttcac aaaagccaca ctatttataa accaggtcga acacccagcg tatggcaaat
     4321 cgcgtaactc atttcagcgc ggttaagcgt atagaagtgg aaatctttca ctccttcacg
     4381 gcttaaaatc ttcaccatat ccatggcaat attcgcgccc accagtttgc gggtttcggc
     4441 atcgtcatcc agcccgtcaa acatttgcgc catccacgcc ggaatacgca cgttggtcat
     4501 gtcggcaaat ttcttcgcct gtttaaagtt agataccggc aaaatgcccg ggatgatttc
     4561 cacatcaatg cccgccgata cacagcggtc acgaaaacgc aggtagcttt cgacatcgaa
     4621 gaagaactga gtaatcgcgc ggttggctcc ggcatccact ttgcgtttca gattaagcaa
     4681 atccgcctga gcgctttttg cttccgggtg aacttccgga tacgccgcca cggatatatc
     4741 gaaatctgcc acttctttta acagcgtcac caggtcagaa gcatacattt ctggcttacc
     4801 acttcccggc ggcagatcgc cacgcagtgc cacgatatga cgaataccgt tattccagta
     4861 gtcgcgtgca atggtgcgca gctcgtcggg cgtcgcatca atgcaagtaa gatgcggtgc
     4921 cgcttccaga ccagtgcgat ctttaatgcc tttaataatg ctgtgcgtac ggtcgcgctc
     4981 gccggagttc gcgccatagg tcaccgatac aaacttcggt ttcaggctgc taaggcgatc
     5041 gatggagttc cacagggtct gctccatttc actggtacgc ggcgggaaaa actcgaacga
     5101 aacgttaatc tgcccctgga cttctgccag gctctgattc agggcatccc gctggctggc
     5161 gtgaaaaaag ctcatacctt acctcatcaa tcgcttgtag ttatatgttg tgttgtgcac
     5221 atctatccat ttagacgtcc agatgtaaag atgaaggaaa agccgaaggg cgtcaaccga
     5281 aaaatgacca atgatggtga ggaaaaatca gggaagatga aaaaacttca ggatggtaag
     5341 aaaaagaaaa tccgatatgc aaagcaaacc ggactgcaaa tataaactaa ccatgtactt
     5401 attgcttctt gccggattcg tcgtaaacgc ttcatccggc ctaattctat gataattcaa
     5461 tatatttcat tagaaaccat agacccgaaa acatgagtac cgggcattat taaatttctg
     5521 aaattacaac aactgtgcca gccggttgat atccgactga atcgccccgg cggtgacgtc
     5581 gcgcccagcg ccaggcccac ggatcaccag cggattatcg cggtaccagc ggctttcgat
     5641 ggcaaagacg ttatcgcatg gcagcagtga tgccagcgga tagtcttcac gcaccgcctc
     5701 cacgcccaca cgcgctttgc cgttggcatc gaaacgcgcc acgtagcgca gcaccagccc
     5761 catttcgcgg gccgcttcca gccgttgcac catctgctcg ttaagttcat cgccattttc
     5821 aaagaaatgg tcgatgctgc cgccttcgca atgagcaggc accagcgatt ccacacgtac
     5881 ctggtccggt tcaatgttgt aacccgcttc acgcgccaga atcaccagct tgcgcattac
     5941 gtctttgccg gagaggtcgt cacgcggatc aggctcggtt aagccctgct gccacgcctg
     6001 atccaccagc tcggtaaacg gcacgctacc gtcgaattgc aggaacagcc aggagagcgt
     6061 gccggagaag atcccactga tcgacaaaat ggtatcgccg ctgtcgatca gatcgcgcac
     6121 ggtatggttg atcggcaagc ccgcaccaac ggtggcgttg tatagccagt gacgcccggt
     6181 tttttcgaag gcgtcgtgga tctggcgata tttattgctg tcgcttgctc ccgccagttt
     6241 gttggcactg atgacgtgga aaccgtggct ggcgaaatcg agatactgat cagcaagctg
     6301 ctggctggcg gtaacgtcca acaccactaa atcatcatac ggatgggcgc gcatccacag
     6361 gaaaagcgac tcttcatcct gctcgaccgc ttcatcgttg aagaaggcta acgcgcggct
     6421 ggcgtccagc ccgtcataac tcaacagact gcggcggctg tccaccacgc ctgccagcac
     6481 aaattcaaag ccggtacgcg ccgaaagcgt gctctgctcg cgggcgaaca gttccagcca
     6541 gcgagagccg atattgcctt tgccgaacaa taccaggccg atgcgttttt ctgcgcggaa
     6601 gacggactga tgcagcccct gaatcaggct ttcggtcggg ccggtgcgca gtactgccac
     6661 cagactgatg ccgtcatcgg actgccacgt aaactcgacc ggctggcctt tcagttgctg
     6721 ccagaagcgg tggcaatgca gcgggttacg ggtgacaccc gccccgacca tcgccaccag
     6781 cgccagcccc tgacgcaggc gcagttctcc aggtaatccc gcttcgtcga ggattttcag
     6841 cgcactgtcg gccacttctg aggtgtagca aaattgcaac aactggcgat cgttatgcac
     6901 gccaaccgcc agtggacgca cctgggcacg tttcaggatc tggtcgatct ctttatgcgc
     6961 cagtttgaaa tcctgactgg cgggcacctg aaactcaatc aaacagacat catcgtggct
     7021 ggtgacaata cgcgcaccag taccggaggc cagcacgcgt tcaatgcgcg tggaaccttg
     7081 atccggcgtg tagctacagc gcagctgcag gtcgatttcg ctgccagaaa ctggctgtaa
     7141 agtacgggcg tgaagaacgg gagccgccag acgcgccagt tcgctggcct catccagacg
     7201 cagcaacggc agcaggcagg catctttcac tttacgcggg tcggcactgt ataccccggc
     7261 gacgtcgctc cagatggtta cacgagaaac acccgccagc gcaccgattt gtgtcgcgga
     7321 atagtcggaa ccgttacgcc ccagcaacac cgtttcaccg gcgttgttac ggctgataaa
     7381 cccggtcacc accagacgtt tgctcggatg ttgtaccagt agctgttgca gcaacgggta
     7441 agaaagccct tcatcaacct gcggttgcgc agcgcgttca gcgcgtaaaa actcacgggc
     7501 atcaagccag gccgctggca gcccttgttg attaagtacc gcagacatca ggcgcgccga
     7561 ccacacttca ccgtggccca ccacttccgc atacaccgca tcgttaatac cactgtcgag
     7621 caacgccgcc aggcgctcaa gatcgctgac aaaagcgcta atgaggctat cggcttcttc
     7681 agcgggtagc agaccgctaa tcagatcgca ctgatagcga cgcagcgttt gttgaacctg
     7741 atgcgcagag agacgatcgg tctggcttag tttcaaccag ttaatcaact ggttagtggt
     7801 gctaccggcg gcggaaacca ccatcatatc gtcaggctga gagaactccg ccataatgcc
     7861 cgcgacacgc aaataacact tcacatcagc cagactactg ccaccaaatt tatgcagctg
     7921 acgacctttc gcccctgcct gcgcaatcac actcattttt accccttgtt tgcagcccgg
     7981 aagccatttt ccaggtcggc aattaaatct tcgccatctt caataccggt ggagatacgc
     8041 agcagcgtct cggagatccc ggcggcagca cgcgcttctg gtgccatgcc tgcatgtgtc
     8101 atggttgcgg cgtgagagat taaactttcc actcccccta atgattccgc cagcgtaaac
     8161 aacgacagcc cgcccaggaa acgacacagc gtctgctcat cgccatccag ttcaaaactc
     8221 aacattgcgc caaagccttt ttgctgacgc gcggcaattt catgcccctg attttccggc
     8281 agcgacgggt gatacagttt tttcaccaac ggctgggttt gcagatattt cacaatcgcc
     8341 tgcgcgttgc gctgcgccag ctccatacgc ggcaccagcg ttcgcaaccc gcgtagcagc
     8401 agatagctgt caaacgcgcc gcccgtcacg ccaatattgt ttgcccacca ggcaagttca
     8461 gtgacaacgt ccgggtcttt agcaatcacc acgccagcca ccacgtcaga gtgaccgttc
     8521 agatatttcg tacatgaatg caacaccaga tcggcaccca atgccagcgg attttgtaat
     8581 gccgggctta agaaggtgtt atccaccacg ctcaccgccc cgacttccct tgccagatgg
     8641 cagattttcg caatatccac gacgcgtaac aatggattac ttgggctttc taccagtacc
     8701 agtttgggtt tttctgccag cgctgcccgt aatgcctgtt catcgccttg atcaacaaac
     8761 aacacgcgat agcaaccgcg tttcgccaga ctgtcgaaca ggcgatagct accgccgtag
     8821 cagtcgtgcg gcgcaaccag cagatcgcca ggtttcaaaa agacggtcgt taccaggtga
     8881 atcgcggaca tgccggtatt agtaagtact gcgccagcac cgccttccag ttctgccagc
     8941 gcacgctgaa ccacatcgcg cgttgggttg ccgcgacgcg agtaatcatg cgcgcgcggt
     9001 tcattaaatc cggtaaagtt ataggtgctg gaaagatgga tgggagggac aacgcaacca
     9061 tactgttcgt cgtcatttaa cccgctacgc actgcgatgg tggcctgttt acgcgtcatg
     9121 tgatgaagtt tcctgggctt tgtcggtgaa atgtcaggca ccagagtaaa cattgtgtta
     9181 atggacgtca atacatctgg acatctaaac ttctttgcgt atagattgag caaatcccaa
     9241 atagccgtta aaattatatg cattatcacg ccgacaggtg cattacacga tgtcacggta
     9301 acgcctgtac ggtaaactat gcgggtttac ggtcagtacc cacataaatt gtggggtctg
     9361 gtctcaattt attgacgaag aggattaagt atctcatggc tgaatggagc ggcgaatata
     9421 tcagcccata cgctgagcac ggcaagaaga gtgaacaagt caaaaagatt acggtttcca
     9481 ttcctcttaa ggtgttaaaa atcctcaccg atgaacgcac gcgtcgtcag gtgaacaacc
     9541 tgcgtcacgc taccaacagc gagctgctgt gcgaagcgtt tctgcatgcc tttaccgggc
     9601 aacctttgcc ggatgatgcc gatctgcgta aagagcgcag cgacgaaatc ccggaagcgg
     9661 caaaagagat catgcgtgag atggggatta acccggagac gtgggaatac taaagcggaa
     9721 aagagcggcg cggagtggaa tcgcctgatg cactacgctt atcaggccta cgtcatattg
     9781 caatttattg aatttgcacg aacttgtagg ccggataagg cgttcacgcc gcatccggca
     9841 taaacaacga gcacgttgtc tgcgacccac cgctttttat acatggacgt ttaactatga
     9901 aaaacaggct gctgatcctc agcctgctgg tttctgtacc tgcctttgcc tggcagccgc
     9961 aaactggcga catcatcttt caaatctctc gctcatcgca aagtaaagcg atccaactgg
    10021 caacccattc cgattacagc cacaccggta tgctggtgat gcgtaacaaa aagccctacg
    10081 tttttgaagc ggtcggcccg gtgaaataca ccccgctcaa gcagtggatt gcccatggtg
    10141 aaaagggcaa ctacgttgtt cgccgcgttg aaggcggact gagtgttgaa caacagcaaa
    10201 aactgacgca aacggcaaaa cgttatctcg gtaaaccgta tgatttcagc ttctcatgga
    10261 gcgacgatcg ccagtactgt tcggaagtgg tgtggaaggt ttaccagaac gcgctgggaa
    10321 tgcgcgtggg cgaacagcag aagttgaaag agtttgatct cagcaacccg ctggtccagg
    10381 cgaagctcaa agaacgctac ggtaaaaata ttccgcttga ggaaacggta gtttcaccac
    10441 aagccgtttt cgacgcgcca caactcacca cggttgccaa agagtggccg ctgttttcgt
    10501 ggtgatacga ccaaaattca ggcacaaaaa aagcgccgtg cggcgctttt ttcggaaatc
    10561 cggtcttatt tgctgcccgg gatgttgaaa cgcttgttga agcggtcaac acggccaccg
    10621 gtagcaacat cacgctgttt gccagtgaag aacgggtggc acttgctgca cacgtcgagg
    10681 ttcaggtcat gaccaacggt ggagcggatt tttattacgt taccgcaaga gcagctagca
    10741 gtaatttctt cgtatttcgg gtgaatatct tttttcatgg gaaaacctcg gtttaaggcc
    10801 gcgtcgctct tccagcccta acgccagaca ccacgcgatg ttaaaagtat agcttcaata
    10861 cgatcatttc gtacgaaggc gcgaaatcat acagaaatta accagcgtat gcaaactgat
    10921 ccgcactctt ctacggcaat gtgtatacta acccaccgaa tttcaagtca ggatgatgct
    10981 atgcccgttg cccacgttgc cttgcccgtt ccgcttcctc gtacctttga ctatctgctg
    11041 ccagaaggca tgacggttaa agctgggtgt cgcgtgcgcg tgccgtttgg caaacagcag
    11101 gagcgcatcg ggattgtggt atcagttagc gatgccagcg aactgccgct cactgagcta
    11161 aaagcggtag tcgaagtgct ggatggtgag ccggtgttta ctcactccgt ctggcgattg
    11221 ctgctatggg cggcagatta ctatcatcat ccgattggcg atgtgctgtt tcatgccttg
    11281 ccgattttac tacgccaggg gcggcctgcg gcgaacgcgc cgatgtggta ctggtttgcc
    11341 actgaacaag gtcaggcggt ggatctgaac agcctgaaac gctcacccaa gcaacaacag
    11401 gcgctggcag cgttacggca aggcaaaatc tggcgcgacc aggttgccac gctcgaattt
    11461 aatgatgccg cgttgcaggc gctgcgcaaa aaaggtctgt gtgatttagc aagtgaaaca
    11521 ccagagttta gcgactggcg aacgaactat gccgtttctg gtgagcggtt gcgattgaat
    11581 accgaacagg ccaccgccgt tggcgcaatt catagcgcgg cagatacttt ttctgcctgg
    11641 ctgctggcgg gcgttaccgg ttccggtaaa acggaggttt atctcagtgt actggaaaac
    11701 gtgctcgctc agggcaaaca ggcgctggtg atggtgccgg aaatcggcct gacaccgcaa
    11761 actatcgccc gttttcgtga acggtttaat gcgcccgtgg aagttctgca ttccggtctg
    11821 aacgacagcg agcgtctttc ggcgtggctg aaagcgaaaa atggcgaggc ggcgattgtg
    11881 atcggcaccc gctccgcgct gtttacgccg tttaaaaatc tcggcgtgat tgtcattgac
    11941 gaagagcacg acagttccta caagcagcag gaaggctggc gctatcatgc ccgcgacctg
    12001 gcggtgtatc gtgcgcatag cgagcaaatc ccgattattc ttggctccgc aacgcccgcg
    12061 ctggaaacgt tgtgcaacgt ccagcagaaa aaataccgcc tgctgcgcct gacgcgccgg
    12121 gcggggaatg cgcgtccggc aattcaacac gtgctggatt taaaaggtca gaaggtgcag
    12181 gcaggtctgg ctccggcgtt aatcacccgt atgcgccagc atttacaggc caacaatcag
    12241 gttatcctct ttcttaaccg ccgtggcttt gcgcctgcgc tactgtgcca cgactgcggc
    12301 tggattgccg aatgcccacg ttgcgatcac tactacacgc tgcatcaggc gcaacagcat
    12361 ttgcgctgtc accactgtga cagtcagcgt ccggtgccgc gccagtgccc ttcctgcggt
    12421 tccacgcacc tggtacccgt ggggctgggc accgaacagc ttgaacagac gcttgcgccg
    12481 ctgttccctg atgtgcccat ttctcgtatc gaccgcgata ccaccagccg caaaggggcg
    12541 ctggaacagc aactggcgga agtacatcgc ggcggtgcga ggattttgat cggcacacaa
    12601 atgctggcga aaggtcacca ctttccggat gtgacgctgg tggcgttact ggatgtggac
    12661 ggcgcgctgt tttctgccga ttttcgctcg gcagagcgtt tcgctcagct ttacacccag
    12721 gtcgctggtc gtgccgggcg tgcgggtaaa caaggcgaag tggtgctgca aacgcaccat
    12781 ccggaacatc ctctgttgca aacgttgctc tataaaggct acgatgcctt tgccgaacag
    12841 gcgctggctg agcgacggat gatgcaactg ccgccatgga ccagccatgt gattgtgcgt
    12901 gcggaagatc ataacaatca gcatgcgccg ctgttcctgc aacaactgcg taatctgatc
    12961 ctctccagcc cgctggcaga cgacaaactg tgggttctcg gtccggttcc ggcactggca
    13021 cctaaacgtg gcggtcgctg gcgctggcag atattgttgc agcacccttc ccgcgtgcgc
    13081 ttgcaacaca tcattagtgg tacgctggcg ctcatcaata caataccgga ttcccgcaag
    13141 gtgaaatggg tactggatgt tgatccgatt gagggttaaa ccgcttacga tgcgaggcgg
    13201 atcgaaaaat tcaatattca tcacactttt catgaaaatt ctgtaaccgt tttcacgcgc
    13261 tatctgctaa aaatgttgcc gatgtgaagt aaacatggat gtagtacgcc tgacgtgcca
    13321 ggcgaggagt gagtgtgaaa gcgaagaagc aggaaactgc cgcgaccatg aaagacgttg
    13381 ccctcaaggc aaaagtctct acagcgactg tctcccgagc attaatgaat cccgataaag
    13441 tctcccaggc cacccgtaat cgggttgaaa aagcggcccg ggaagtgggt tatttaccgc
    13501 agcctatggg gcgcaacgtc aagcgtaatg aatcccgcac cattctggtg attgtcccgg
    13561 atatctgcga tcccttcttt agcgaaatta ttcgcggtat cgaagttacg gcggcaaatc
    13621 acggatatct ggtgctgatt ggcgactgtg cgcatcaaaa tcagcaggaa aaaaccttta
    13681 tcgatttgat catcaccaag caaattgatg gcatgttgct gctgggttca aggctgccgt
    13741 ttgatgccag cattgaggaa cagcgtaatc tgccgccgat ggtgatggcg aacgaatttg
    13801 caccggaact ggagctgcct accgttcata tcgacaatct gaccgccgca tttgatgcag
    13861 taaattattt atatgagcaa gggcataaac ggattggctg tatagccggt cccgaagaga
    13921 tgccactgtg tcactaccgc ctgcaaggct atgttcaggc gctgcgtcgc tgcggaatta
    13981 tggttgatcc acaatacatc gcccgtggcg acttcacctt cgaagccgga agcaaagcaa
    14041 tgcaacagct gcttgatctt ccacaaccgc ctactgcagt cttctgccat agcgatgtga
    14101 tggcgctcgg cgcactttct caggcaaaac gccaggggct gaaagtcccg gaagaccttt
    14161 ccataatcgg ttttgataac atcgacctga cgcaattttg tgatccgccg ctgacaacca
    14221 tcgcgcagcc gcgttacgaa atcggtcggg aagctatgct gttattgctt gatcaaatgc
    14281 aggggcaaca cgttggcagt ggctctcgtt taatggactg cgaacttatc atccggggat
    14341 caacacgcgc gttaccttaa agtaaacctt taagactgcc gtgactggtc aaaggcccgc
    14401 cgcttaagta acatggcggg ctgacgaacg aataaataca gcgaaacgat agtggcacaa
    14461 cgagattatg tacgccgcag ccaaccggca ccttcgcggc gaaaaaagag cacctcacgg
    14521 aaaaagcaac gaaatctgcc tgcggtttct cccgctatgg tcgctattgc tgccgccgtt
    14581 cttgtgacct ttatcggtgg tctgtacttc attacgcatc acaagaaaga agagtccgag
    14641 acgctgcaaa gccagaaagt gaccggaaac ggactaccac caaaaccaga agaacgctgg
    14701 cgctacatta aagagctgga aagtcgccag ccgggtgtgc gtgcgcccac agaaccttct
    14761 gccggtggtg aagtgaaaac gccggagcaa ctgacaccag aacaacgtca gcttcttgaa
    14821 caaatgcagg ctgatatgcg ccagcagcca acgcagctgg ttgaggttcc gtggaatgaa
    14881 cagacgccgg aacagcgtca gcaaacgcta caacgccagc gtcaggcgca gcaattagcg
    14941 gagcagcaac gcctggctca gcagtcgcgt acgactgaac aaagctggca gcagcagacg
    15001 cgtacgtcgc aagccgcgcc ggtacaggcg caaccgcgcc agtctaaacc agcttctact
    15061 cagcagccgt accaggatct gctgcaaacc cctgcgcaca cgaccgcgca gtcgaagccg
    15121 cagcaagctg cgccggttgc tcgtgccgct gacgcgccaa aaccgacggc ggagaaaaaa
    15181 gacgaacgcc gctggatggt gcagtgcggt tcgttcagag gcgcggaaca ggcagagacg
    15241 gtacgtgctc agctggcgtt cgaaggattt gactcgaaaa tcaccaccaa caatggctgg
    15301 aatcgtgtgg tcattggccc ggtgaaaggc aaagagaacg cagacagcac cctcaatcgg
    15361 ttgaagatgg cgggtcatac aaactgcatt cggctcgccg ccgggggttg aaaccctcaa
    15421 aatccccccc atctataatt gcattatgcc ccgtactttt gtacggggtt tgtactctgt
    15481 attcgtaacc aaggggtcag ctcgtgacaa ctatagtaag cgtacgccgt aacggccatg
    15541 tggtcatcgc tggtgatggt caggccacgt tgggcaatac cgtaatgaaa ggcaacgtga
    15601 aaaaggtccg ccgtctgtac aacgacaaag tcatcgcggg ctttgcgggc ggtactgcgg
    15661 atgcttttac gctgttcgaa ctgtttgaac gtaaactgga aatgcatcag ggccatctgg
    15721 ttaaagccgc cgttgagctg gcaaaagact ggcgtaccga tcgcatgctg cgcaaacttg
    15781 aagcactgct ggcagtcgcg gatgaaactg catcgcttat catcaccggt aacggtgacg
    15841 tggtgcagcc agaaaacgat cttattgcta tcggctccgg cggcccttac gcccaggctg
    15901 cggcgcgcgc gctgttagaa aacactgaac ttagcgcccg tgaaattgct gaaaaggcgt
    15961 tggatattgc aggcgacatt tgcatctata ccaaccattt ccacaccatc gaagaattaa
    16021 gctacaaagc gtaaggatct cccatgtctg aaatgacccc acgcgaaatc gtcagcgaac
    16081 tggataagca catcatcggc caggacaacg ccaagcgttc tgtggcgatt gctctgcgta
    16141 accgctggcg tcgcatgcag ctcaacgaag agctgcgcca tgaagtgacc ccgaaaaata
    16201 tcctgatgat cggcccgacc ggtgtcggta aaactgaaat cgcccgtcgt ctggctaagc
    16261 tggcgaatgc gccgttcatc aaagttgaag cgaccaaatt taccgaagtg ggctacgtcg
    16321 gtaaggaagt tgattctatt attcgcgatc tgaccgatgc cgccgtgaaa atggtacgcg
    16381 tccaggctat cgagaaaaac cgttatcgcg ctgaagaact ggcagaagaa cgtattctcg
    16441 acgtgctgat cccacctgct aaaaacaact ggggacagac tgaacagcag caggaacctt
    16501 ccgctgctcg tcaggcattc cgcaaaaaac tgcgtgaagg ccagcttgat gacaaagaaa
    16561 tcgagatcga tcttgccgca gcaccgatgg gcgttgaaat tatggctcct ccgggcatgg
    16621 aagagatgac cagccagctg cagtccatgt tccagaacct gggcggccag aagcaaaaag
    16681 cgcgtaagct gaaaatcaaa gacgccatga agctgctgat tgaagaagaa gcggcgaaac
    16741 tggtgaaccc ggaagagctg aagcaagacg ctatcgacgc tgttgagcag cacgggatcg
    16801 tgtttatcga cgaaatcgac aaaatctgta agcgcggcga gtcttccggt ccggatgttt
    16861 ctcgtgaagg cgttcagcgt gacctgctgc cgctggtaga aggttgcacc gtttccacca
    16921 aacacgggat ggtcaaaact gaccacattc tgtttatcgc ttctggcgcg ttccagattg
    16981 cgaaaccgtc tgacctgatc ccggaactgc aaggtcggct gccaatccgc gttgaactgc
    17041 aggcactgac caccagcgac ttcgagcgta ttctgaccga gccgaatgcc tctatcaccg
    17101 tgcagtacaa agcactgatg gcgactgaag gcgtaaatat cgagtttacc gactccggta
    17161 ttaaacgcat cgcggaagcg gcatggcagg tgaacgaatc taccgaaaac atcggtgctc
    17221 gtcgtttaca cactgttctg gagcgtttaa tggaagagat ttcctacgac gccagcgatt
    17281 taagcggtca aaatatcact attgacgcag attatgtgag caaacatctg gatgcgttgg
    17341 tggcagatga agatctgagc cgttttatcc tataatcgcg ttcaatcatt ttcatcattg
    17401 tttgatgggg ctgaaaggcc ccatttttat tggcgcgtat tatgactgaa caacaaatta
    17461 gccgaactca ggcgtggctg gaaagtttac gacctaaaac cctccccctc gcctttgctg
    17521 caattatcgt cgggacggcg ctggcatggt ggcaaggtca cttcgatccg ctggtcgccc
    17581 tgctggcact aattaccgcc gggctattac agatcctttc taacctcgcc aatgattacg
    17641 gcgatgcggt aaaaggcagc gataaacctg accgcattgg gccgctacgc ggcatgcaaa
    17701 aaggggtcat tacccagcaa aagatgaaac gggcgctcat tattaccgtc gtgctcatct
    17761 gtctctccgg gctggcactg gttgcagtgg catgccatac gctggccgat tttgtcggtt
    17821 tcctgattct tggcgggttg tcgatcattg ccgctatcac ctacaccgtg ggcaatcgtc
    17881 cttatggtta tatcggtctg ggtgatattt ccgtactggt tttctttggc tggttgagtg
    17941 tcatggggag ctggtattta caggctcata cattgattcc ggcactgatc cttccggcga
    18001 ccgcatgcgg cctgctggca acggcagtac tgaatattaa taacctgcgt gatatcaata
    18061 gcgaccgcga aaatggcaaa aacacgctgg tggtgcgctt aggtgaagtg aacgcgcgtc
    18121 gttatcatgc ctgcctgctg atgggctcgc tggtgtgtct ggcgctgttt aatctctttt
    18181 cgctgcatag cctgtggggc tggctgttcc tgctggcggc accattactg gtgaagcaag
    18241 cccgttatgt gatgcgggaa atggacccgg tggcgatgcg accaatgctg gaacgtactg
    18301 tcaagggagc gttactgact aacctgctgt ttgttttagg gatattccta agccagtggg
    18361 cagcataact gacaaatatc aattaacaat tgatgatttt gccaacagcc cacatagcgc
    18421 gatatactga aaattctcgc agcaactgaa tgttaagcct atgaaatacg atacttccga
    18481 gctttgtgac atctatcaag aagatgttaa cgtcgtggaa ccgctgttct ccaactttgg
    18541 cggacgggcg tcgtttggcg gacaaataat cacggtaaaa tgtttcgagg acaacgggtt
    18601 gctgtacgat ctgctcgaac agaatggccg tggtcgtgtt cttgtcgtcg atggcggtgg
    18661 ttctgttcgt cgcgcactgg tcgatgctga actggcgcgt ctggcagtac aaaatgaatg
    18721 ggaaggtctg gtcatttacg gcgcggtgcg tcaggtagat gacctggaag agttggatat
    18781 cggcatccag gcgatggcgg caattccggt tggtgccgct ggcgaaggca ttggcgaaag
    18841 cgatgtccgc gtcaattttg gcggtgtcac cttcttctcc ggcgaccatc tttatgccga
    18901 caataccggg attattcttt cagaagatcc gctggatatt gaatgataag aaaggcaccg
    18961 caaggtgcct tttttctgcg ttacctgttg gcctacacag taaagaaatt acgcgaaaga
    19021 tgaagcgtaa tcagacctct tccatgcgac ccagcagggc ctgcagacgt tcctgccagc
    19081 cgttctgctg ttctttcaga tggttgttct cacgctccag ctcttcgcgc tgatgctggg
    19141 cattttgaac ttcctgcgac agtgagttgt ttttttcttt cagctcttcg atttccatct
    19201 gcaacagagt gatggtatca atcgcctgct gtacttttgc ttccagtttc tcaaacactt
    19261 ctaatgacat tgtcatacct ctcctgaatt gcaaggcgtt gatggataaa aaatcctcgt
    19321 cccgattacc ggtgacgcct taataaatac gagcgcactt tagttagctc cgattgtatg
    19381 aagccgcgcc atcgctgtcc agcggcacgc cttgcagatt acggtttgcc acacttttca
    19441 tccttctcct agtgacataa tccacatcaa tcgaaaatgt taataaattt gttgcgcgaa
    19501 tgatctaaca aacatgcatc atgtacaatc agatggaata aatggcgcga taacgctcat
    19561 tttatgacga agcacacaca ttttaagttc gatatttctc gtttttgctc gttaacgata
    19621 agttaacagc atgcctacaa gcatcgtgga ggtccgtgac tttcacgcat acaacaaaca
    19681 ttaactcttc aggatccgat tatgagtcaa acatcaacct tgaaaggcca gtgcattgct
    19741 gaattcctcg gtaccgggtt gttgattttt ttcggtgtgg gttgcgttgc agcactaaaa
    19801 gtcgctggtg cgtcttttgg tcagtgggaa atcagtgtca tttggggact gggggtggca
    19861 atggccatct acctgaccgc aggggtttcc ggcgcgcatc ttaatcccgc tgttaccatt
    19921 gcattgtggc tgtttgcctg tttcgacaag cgcaaagtta ttccttttat cgtttcacaa
    19981 gttgccggcg ctttctgcgc tgcggcttta gtttacgggc tttactacaa tttatttttc
    20041 gacttcgagc agactcatca cattgttcgc ggcagcgttg aaagtgttga tctggctggc
    20101 actttctcta cttaccctaa tcctcatatc aattttgtgc aggctttcgc agttgagatg
    20161 gtgattaccg ctattctgat ggggctgatc ctggcgttaa cggacgatgg caacggtgta
    20221 ccacgcggcc ctttggctcc cttgctgatt ggtctactga ttgcggtcat tggcgcatct
    20281 atgggcccat tgacaggttt tgccatgaac ccagcgcgtg acttcggtcc gaaagtcttt
    20341 gcctagctgg cgggctgggg caatgtcgcc tttaccggcg gcagagacat tccttacttc
    20401 ctggtgccgc ttttcagccc tatcgttggc gcgattgtag gtgcatttgc ctaccgcaaa
    20461 ctgattggtc gccatttgcc ttgcgatatc tgtgttgtgg aagaaaagga aaccacaact
    20521 ccttcagaac aaaaagcttc gctgtaatat gactacggga caattaaaca tgactgaaaa
    20581 aaatatatcg ttgcgctcga ccagggcacc accagctccc gcgcggtcgt aatggatcac
    20641 gatgccaata tcattagcgt gtcgcagcgc gaatttgagc aaatctaccc aaaaccaggt
    20701 tgggtagaac acgacccaat ggaaatctgg gccacccaaa gctccacgct ggtagaagtg
    20761 ctggcgaaag ccgatatcag ttccgatcaa attgcagcta tcggtattac gaaccagcgt
    20821 gaaaccacta ttgtctggga aaaagaaacc ggcaagccta tctataacgc cattgtctgg
    20881 cagtgccgtc gtaccgcaga aatctgcgag catttaaaac gtgacggttt agaagattat
    20941 atccgcagca ataccggtct ggtgattgac ccgtactttt ctggcaccaa agtgaagtgg
    21001 atcctcgacc atgtggaagg ctctcacgag cgtgcacgtc gtggtgaatt gctgtttggt
    21061 acggttgata cgtggcttat ctggaaaatg actcagggcc gtgtccatgt gaccgattac
    21121 accaacgcct ctcgtaccat gttgttcaac atccataccc tggactggga cgacaaaatg
    21181 ctggaagtgc tggatattcc gcgcgagatg ctgccagaag tgcgtcgttc ttccgaagta
    21241 tacggtcaga ctaacattgg cggcaaaggc ggcacgcgta ttccaatctc cgggatcgcc
    21301 ggtgaccagc aggccgcgct gtttggtcag ttgtgcgtga aagaagggat ggcgaagaac
    21361 acctatggca ctggctgctt tatgctgatg aacactggcg agaaagcggt gaaatcagaa
    21421 aacggcctgc tgaccaccat cgcctgcggc ccgactggcg aagtgaacta tgcgttggaa
    21481 ggtgcggtgt ttatggcagg cgcatccatt cagtggctgc gcgatgagat gaagttgatt
    21541 aacgacgcct acgattccga atatttcgcc accaaagtgc aaaacaccaa tggtgtgtat
    21601 gtggttccgg catttaccgg gctgggtgcg ccgtactggg acccgtatgc gcgcggggcg
    21661 attttcggtc tgactcgtgg ggtgaacgct aaccacatta tacgcgcgac gctggagtct
    21721 attgcttatc agacgcgtga cgtgctggaa gcgatgcagg ccgactctgg tatccgtctg
    21781 cacgccctgc gcgtggatgg tggcgcagta gcaaacaatt tcctgatgca gttccagtcc
    21841 gatattctcg gcacccgcgt tgagcgcccg gaagtgcgcg aagtcaccgc attgggtgcg
    21901 gcctatctcg caggcctggc ggttggcttc tggcagaacc tcgacgagct gcaagagaaa
    21961 gcggtgattg agcgcgagtt ccgtccaggc atcgaaacca ctgagcgtaa ttaccgttac
    22021 gcaggctgga aaaaagcggt caaacgcgcg atggcgtggg aagaacacga cgagtaatgt
    22081 caggcacctg aatcgattgc ctgatgctga tcgcctgatg cgacgcttac gcgtcttatc
    22141 aggcctacga gccaatgcca ttttgtgtag gcgggataag gcgttcacgc cgcatccggc
    22201 atcacgcaga ctctgaaacc gtgcgggggc aaccccgcac acatcaataa tcccaccctt
    22261 cccctgtgct acacttcgcg ccattcctta ctgcttagag tttgctatga gacgagaact
    22321 tgccatcgaa ttttcccgcg tcaccgaatc tgcggcgctg gctggctaca aatggttagg
    22381 acgcggcgat aaaaacaccg cggacggcgc ggcggtaaac gccatgcgta ttatgctcaa
    22441 ccaggtcaac attgacggca ccatcgtcat tggtgaaggt gaaatcgacg aagcaccgat
    22501 gctctacatt ggtgaaaaag tcggtactgg tcgcggcgac gcggtagata ttgctgttga
    22561 tccgattgaa ggcacgcgca tgacggcgat gggccaggct aacgcgctgg cggtgctggc
    22621 agtaggcgat aaaggctgct tcctcaatgc gccggatatg tatatggaga agctgattgt
    22681 cgggccggga gccaaaggca ccattgatct gaacctgccg ctggcggata acctgcgcaa
    22741 tgtagcggcg gcgctcggca aaccgttgag cgaactgacg gtaacgattc tggctaaacc
    22801 acgccacgat gccgttatcg ctgaaatgca gcaactcggc gtacgcgtat ttgctattcc
    22861 ggacggcgac gttgcggcct caattctcac ctgtatgcca gacagcgaag ttgacgtgct
    22921 gtacggtatt ggtggcgcgc cggaaggcgt ggtttctgcg gcggtgatcc gcgcattaga
    22981 tggcgacatg aacggtcgtc tgctggcgcg tcatgacgtc aaaggcgaca acgaagagaa
    23041 tcgtcgcatt ggcgagcagg agctggcacg ctgcaaagcg atgggcatcg aagccggtaa
    23101 agtattgcgt ctgggcgata tggcgcgcag cgataacgtc atcttctctg ccaccggtat
    23161 taccaaaggc gatctgctgg aaggtattag ccgcaaaggc aatatcgcga ctaccgaaac
    23221 gctgctgatc cgcggcaagt cacgcaccat tcgccgcatt cagtccatcc actatctgga
    23281 tcgcaaagac ccggaaatgc aggtgcacat tctctgagtg atttgatcga ttgagccttc
    23341 cagtccttcg ggactggaat ttttttgttc ggagaacgaa gataaggcaa gtcaatcaaa
    23401 acaggagaaa aacatggctg attgggtaac aggcaaagtc actaaagtgc agaactggac
    23461 cgacgccctg tttagtctca ccgttcacgc ccccgtgctt ccgtttaccg ccgggcaatt
    23521 taccaagctt ggccttgaaa tcgacggcga acgcgtccag cgcgcctact cctatgttaa
    23581 ctcgcccgat aatcccgatc tggagtttta cctggtcacc gtccccgatg gcaaattaag
    23641 cccacgattg gcggcactga aaccgggcga tgaagtgcag gtggttagcg aagcagctgg
    23701 cttctttgtt ctggatgaag taccggactg cgaaacgcta tggatgctgg caaccggtac
    23761 agcgattggc ccttatttat cgattctgca actaggcaaa gatttagatc gcttcaaaaa
    23821 tctggtcctg gttcacgccg cacgttatgc cgccgactta agctatttgc cactgatgca
    23881 agaactggaa aaacgctacg aaggcaaact gcgcattcag acggtggtca gtcgggaaac
    23941 ggcagcgggg tcgctcaccg gacggatacc ggcattaatt gaaagtgggg aactggaaag
    24001 cgcgattggc ctgccgatga ataaagaaac cagccatgtg atgctgtgcg gcaatccaca
    24061 gatggtgcgc gatacacaac agttgctgaa agagacccgg cagatgacga aacatttacg
    24121 tcgccgaccg ggccatatga cagcggagca ttactggtaa gcggttactt atcgataaac
    24181 ggcacgatga gcaaatccgc actcatctta ttgatcatcc cgcgatatgc cggcatcaaa
    24241 cggttgataa atgagtgatg atgaccacag acaaggaggt cgcactgctc tttttgcata
    24301 atttccagca gtgtttccgg catttctccg cgttcaatac gcagttttgt cttcggccat
    24361 tgaatatttt tcgtcagttt atacagcttg ttatccgact tattcttcaa caattgaaga
    24421 atatcttctg ttgcagggaa gtagataccc ggatacaact cgcttaagcc atcatcaata
    24481 tgaattaacg tcaggtgagc gtcattatgt ctggcgagct ccagggcttt attcaccagt
    24541 aaggcatctt cttcattccc ggaaattgcc acgccaatgt gtttataagc catgtttaac
    24601 tccttctaaa gcaactccat caagctaacg aacgcagtga taactcaaaa ataatcatct
    24661 cggcctggca tgagaaagtg aaggccgcat caagctcaac ttgcccctcc agatccttaa
    24721 tcttataggc cacgctggcc ccttcatctg cagaggatgc agcggcggca agctctttca
    24781 gtttggtcat aaaagactct gcttcttcgc gggtggcaaa aaagcgcgaa aacttactgg
    24841 tacagttgtc gttatcaatc accgtaccga tatcaatcgc acatccttta gtactgcatt
    24901 tatctacgac atctttcata gggcacctct gtatattcgc ctctctgtcg attacgactt
    24961 aagaataatg cgccgttaag ggaagaacaa acaagtaatg atcttatgga ttaccatttt
    25021 ttttgatatt gcttaaggtg aagagttcag aaggattttc cgggtattgt catttgcatc
    25081 gtggaaataa caatcccgga aaaaaatagc agtaagagat atattaaagg ttaattactt
    25141 gatttattgt cggctttata cttcacatcc tgagtatctt taccatattt attttcgcct
    25201 tgtgtgccaa caaacgcacc aagatcgata agcatcatca ccagaatcaa cgtcgggaca
    25261 aaacgcccca ccgcccattg ccagacaccc ggtaaaatcg ctcagttacc cgccagcagc
    25321 atccacgcca caatcatcag aaatgcccat gcgccggaac gcccgcgatc atgcaagcgc
    25381 ttaacagtta ctgccgctgt tggccagagc aagcacacaa ggcaaaacgc cgcggtctga
    25441 atatcgagta gattcttacc cgccagtgag aaaagcacca gcatgcctgc gaaccacagg
    25501 cctatccaaa tccagaaatc acggcgtcca atacgccctt taaatgagaa taaccattgc
    25561 tgtatggtca tgtaagttcc ttgatggttg tcttttccag gattctaccc ttttgacaag
    25621 ggcgacagat atcgttttaa tcggagccag tcataacaaa aggtactgtc aatgaagcca
    25681 gggtgtacgc tgttttttct cttatgttct gcattaaccg ttacaacaac ggcgcatgca
    25741 caaacaccag atacggcaac gaccgcgcct tatctgctgg ctggagcccc tactttcgat
    25801 ctctccatca gccagtttcg agaagacttt aacagccaga atcccagcct gccactgaac
    25861 gaatttcgtg ccatcgacag cagtcccgac aaagccaatc tcactcgtgc tgcaagtaaa
    25921 attaacgaga acttgtatgc ttctacagcg ctggagcgcg gtaccttaaa aatcaaaagc
    25981 attcaaatga cctggctacc catccagggg ccagagcaaa aagcagcgaa agcgaaagct
    26041 caggaataca tggcagcagt gatccgcaca ctcaccccat taatgaccaa aacacaaagc
    26101 cagaaaaaac tgcagtcgct actaacggcg gggaaaaaca aacgttatta caccgagaca
    26161 gaaggtgcac tgcgttatgt tgtcgcggac aacggcgaaa aggggctgac cttcgctgtt
    26221 gaaccgatta agctggcgct atctgaatcg cttgaaggtt tgaataaatg acaaaaagca
    26281 aagcctttgt gccgatgaat ctctatactg tttcacagac ctgctgccct gcggggcggc
    26341 catcttcctt tattcgctta taagcgtgga gaattaaaat gcgacatcct ttagtgatgg
    26401 gtaactggaa actgaacggc agccgccaca tggttcacga gctggtttct aacctgcgta
    26461 aagagctggc aggtgttgct ggctgtgcgg ttgcaatcgc accaccggaa atgtacatcg
    26521 atatggcgaa gcgcgaagct gaaggcagcc acatcatgct gggtgcgcaa aacgtggacc
    26581 tgaacctgtc cggcgcattc accggtgaaa cttctgccgc tatgctgaaa gacatcggcg
    26641 ctcagtacat catcatcggc cactctgaac gtcgtactta ccacaaagag tctgacgaac
    26701 tgattgcgaa aaaattcgcg gtgctgaaag agcagggtct gactccggtt ctgtgcatcg
    26761 gtgaaaccga agctgaaaac gaagcgggaa aaactgaaga agtttgcgca cgtcagatcg
    26821 acgcggtact gaaaactcag ggtgctgcgg cattcgaagg tgcggttatc gcttacgaac
    26881 ctgtatgggc aatcggtact ggcaaatctg caactccggc tcaggcacag gctgttcaca
    26941 aattcatccg tgaccacatc gctaaagttg acgctaacat cgctgaacaa gtgatcattc
    27001 agtacggcgg ctctgtaaac gcgtctaacg ctgcagaact gtttgctcag ccagacatcg
    27061 acggcgcgct ggttggcggt gcttctctga aagctgacgc tttcgcagtg atcgttaaag
    27121 ctgcagaagc ggctaaacag gcttaagtct gacaggtgcc ggatttcata tccggcactt
    27181 actttcctta actcctcgcc ttaacgcaaa atctcacact catgatcctg gatttcctcg
    27241 gctgaagcac ggttcagcgt cagtagattt cgttgtgtcg ccagcaacac aaacgaatta
    27301 tcactctgcc gtaccatcgc cagcccgtag cttcccatat gttcccgcgc ctcaggcact
    27361 tcttctgcaa gcatcataaa tgggctgcgt tgtaccagtt cgctttccgt tacccgacgc
    27421 gccaggtatt catgcccgcg caaaccacct ggcagtggca accagcggct gctgatgttc
    27481 gccagatttt tatccagttg ttcgcgcaca tcaggacgaa tacaagagat atgaatatga
    27541 aaatggtttt gcgtatgccc ggtgcgggag ttgatcgcca aagaaaccgc gcgatcggga
    27601 accggctggc cgtatttttt gctcataaaa tcacgcgcct gccaggccaa ccaaaagaag
    27661 ttcggcgttg aaggatcggt caacaaagga ctttcagtac cgttaatacg atacgttggc
    27721 atcaacagat attgcagtgg accattaaga tcttttaaaa ccacgtatcc ggcattgggt
    27781 ttgacttccg cacatggcga aggattttga ttttgctgct gattgggcaa acattcctca
    27841 aggacaatct tacgtaatgt atccgactct tcaccggtta atttccagta accaataccg
    27901 gcagccacaa cggcgataac tatcatcacc aaaaaaagaa gacccgcttt tttcatcttt
    27961 ttttccctgt acctcaaaga gatgcaaggg taacgcaaaa tcgtgacaaa taaaaaaccc
    28021 ggtcgcgaga aaccaccggg tttgataatt atcctaggga aatcagcgtt tgctgatctg
    28081 atcgaacgta ccgccgttag caaaatgctc tttttgcgct ttcgtccagc cgccgaactc
    28141 ttcatcaatg gtgaataact tcagctttgg aaacgcattt tcgtactttt tcgctacttc
    28201 agcgtcgcgc ggacggtagt agtttttcgc ggcaatttcc tgaccttctg gcgagtagag
    28261 atatttcagg taggcttccg ccacctcttt ggtgcctttt ttctcgacca ctttatcgac
    28321 caccgacacc gttggctctg cgaggataga ctcactcggc gtgacgattt cgaatttatc
    28381 tttccccagt tcattcgctg ccagcagagc ttcgttttcc caggcgatca gtacatcgcc
    28441 aatcccgcgc tcgacaaagg tgttagtgga gccgcgcgcg ccagaatcca gaacttcgac
    28501 gtttttatac agtgcccgaa caaaatcctg tgcttttgcc tgatcgttgt tgttgtgatg
    28561 cagcgcgtag ccccaggcag ccaggtagtt ccagcgtgcg ccaccagagc ttttcggatt
    28621 aggcgtgatc accgaaacac ccggtttaat cagatcgttc cagtcatgga tctgcttcgg
    28681 attgccctta cgcaccagga aaacaatggt ggaagtgtac ggtgcggagt tatccggcag
    28741 acgtttgatc cactctttat caatccgccc gcgttccgca atcgcgtcca cgtcataggc
    28801 cagagccagc gtgacaacat cagcttcaat accgttgatt accgacgtcg cttgtttacc
    28861 tgaaccaccg tgtgactgac gaatcaccac gttatcacca gtttgctgtt tccagtgggc
    28921 gctgaatgcc ttgttgtact gttcgtacaa ttcgcgcgtt ggatcatatg aaacgttaag
    28981 aagctgaata tcctttgcca taacgctggt tgccgccagc aaaaatgtta accctacgcc
    29041 ccacttgttc atcgcccgac tctcttatgt tgtgttgtga tgagcaaagc gtgccagaag
    29101 gttaaccaaa cattaaagaa taaaaaaaga ttggctataa cttgcgggta tatgttgagg
    29161 gattaaaaag gcggaagaaa tccgcctcat attgctgaca aagtgcgctt tgtccatgcc
    29221 ggatgcggcg cgaacgcctt atccggccta caaaagtttg caaattcaat aaattgcaga
    29281 attcatgtag gcctgataag cgaagcgcat caggcatttt tgcttctgtc atcggtttca
    29341 ggctaaagga atctgccttt ttccgaaatc attaatacag ttttttcgcg cagtccagcc
    29401 agtcaccttt gaacggacgc ttcatgtttt cgatagcgtc gatgatgtcg tggtgaacca
    29461 gctgttcgtt ctggatacct acacaacgac cgccgtaacc tgccagcagc agatcgatag
    29521 cgtaagcgcc catacgggaa gccagaatac ggtcgtaagg caccggagaa ccaccgcgct
    29581 ggatgtggcc cagcacagtt gcgcgggttt cacgaccggt ttctttctcg atgaaatgcg
    29641 ccagttcgtc aacatcacac atatgttcgg taatcgccac gatcgcgtgt tttttacctt
    29701 tcgcgatacc cgctttgatt tcgtttacca ggtcttcacg gctgaattca acttccggaa
    29761 ctacaacgaa ttcacagcca ccggcaatgg ccgcagccag ggtcagatcg ccacaataac
    29821 ggcccatcac ttccaccacg gaaatacgct ggtgagaaga agaggtgtca cgcagacggt
    29881 cgatcgcttc tacaacggtg ctcagcgcag tgaagaaacc gatagtgtag tcagtgcctt
    29941 tgatgtcgtt gtcgatagtg cccggcagac cgatgcacgg gaagcccatt tcggtcagac
    30001 gcattgcacc catgtaggaa ccgtcaccgc cgataaccac cagcgcgtcg ataccacgtt
    30061 ttttcaggtt ttcgatagcc acggcgcgga tgttctcgtc gcggaattcc gggaaacgcg
    30121 cagaaccgag gaacgtaccg ccacggttga tcatgtcaga cacgctgtaa cggtctagct
    30181 gtaccatacg gtcttcatac agacccagat agccgtcata aatacccatt acttccagac
    30241 cttctgtcag cgcagaacga acaaccccgc gaattgcggc gttcatgcct ggcgcatcac
    30301 cgccgcttgt caacacacct attttcttaa tcatgactac ctctgaactt tggaatgcaa
    30361 aatgaaatct gttgccggaa gtcttcttgc acatcgaagt gatccaacga atgtgcaaat
    30421 agtataacaa tcacttcctg ctgaattgat tcaggtcagg ccaaatggcg gtattttata
    30481 cacaaaatgc gggtctggct ctcttttata ctgattatga aagcatagac cgtttaccct
    30541 ccctgggtac gacggaacag gggtcctgat ggataatcac atccgatccc ggaaaacgcc
    30601 gtaaaatagc ctgctctacc tgatccgcca ccatatgtgc ctgaaccaaa ggcagagagt
    30661 cttccatttc caaatgaatc tgaataaagc gggtcggccc tgactgccgc gtgcgaagat
    30721 cgtgagcgcc gctaacaccc ggccaggaag tcacgatatc aataatttct tgccgttctt
    30781 catcaggcaa tgcgcgatcc agtaatgact gtaccgcctc atatcccatg cgtaacgcgc
    30841 tatataaaat atagatgccg attcccaatg caaacagagc atcggcgcga tgccagccgt
    30901 accaagacaa ccccagcgcc agcagaattg cgccgttcat cataacatca gactggtaat
    30961 gtagcatatc agcccgcacc gcctggcttt gcgtccggcg caccacccac cgctgaaacg
    31021 agacaaggat aatcgtacaa attagcgcca caattgtcac gataaccccg acgcctggat
    31081 ctgtcatcgg tgttggagat accagatgtt gaatacccgt caaaaacagg aatagtgccg
    31141 aaccggagat aaacatactt tgcgccagcg ccgcgaggga ctcagcttta ccgtgaccaa
    31201 acgagtgatt atcgtcggca ggttgcaggg aatatcgcac caccagtaaa ttcgtcaacg
    31261 acgcgccgat atccaccagc gaatccacca gcgcggcgag aatactcacc gacccggtat
    31321 accaccatgc aaaaatttta atcagcaata gcaacgaagc catcgccgtc gcagcaatcg
    31381 ccgcccgact gaccagccgt ccataagatt gattcataaa tactcccgct atcaactgac
    31441 gctagtataa cggaagcaaa tcatctgcaa tgcattaagc agcaggcaaa ttgaggataa
    31501 aaaaaacccc acatcatgtg ggggaagaca gggatggtgt ctatggcaag gaaaacaggg
    31561 tttactactg ggaacgtgag ttgctactac tcaatagctt caacgatgaa cttttttgcc
    31621 attgcgtcac gtcacgcaac tgctccattc gttgttgatg tttctcgttt aaaaccgctt
    31681 gctgctccgg cgttaacagg cgatacattt ggttgcggac ttttgccatc tcaacctgac
    31741 gagcaatttg ctcattcgcc attttttctg cctgtgcgcg cacagcgttt tcatcaaaat
    31801 tttctgcggt gacaaggcga tgcattgtct ccagttcgct aacattaaca ggaggctgtt
    31861 cgtgccgggc ctgttgcata agatctcgca tctgctgacg ctgatgttcg gttaaactta
    31921 tgccgtcgaa catatggctc tgcgtactgc gctgcgtaag ttcttcaccc ggatgccagt
    31981 tatcgcctga accgacttca gcagcgtggc ttaatgaact gactgccagc gttgaggcca
    32041 tgacggcagc ggtaactatg cgcatcattt gctcccaaaa tctttctgtc gcgattcaac
    32101 gatagagagt ttacgattca ggctgcaaac atgcgtcagg gggtgtaaaa caacgtaaag
    32161 tcatggatta gcgacgtctg atgacgtaat ttctgcctcg gaggtattta aacaatgaat
    32221 aaaatcctgt tagttgatga tgaccgagag ctgacttccc tattaaagga gctgctcgag
    32281 atggaaggct tcaacgtgat tgttgcccac gatggggaac aggcgcttga tcttctggac
    32341 gacagcattg atttactttt gcttgacgta atgatgccga agaaaaatgg tatcgacaca
    32401 ttaaaagcac ttcgccagac acaccagacg cctgtcatta tgttgacggc gcgcggcagt
    32461 gaacttgatc gcgttctcgg ccttgagctg ggcgcagatg actatctccc gaaaccgttt
    32521 aatgatcgtg agctggtggc acgtattcgc gcaatcctgc gccgttcgca ctggagcgag
    32581 caacagcaaa acaacgacaa cggttcaccg acactggaag ttgatgcctt agtgctgaat
    32641 ccaggccgtc aggaagccag cttcgacggg caaacgctgg agttaaccgg tactgagttt
    32701 accctgctct atttgctggc acagcatctg ggtcaggtgg tttcccgtga acatttaagc
    32761 caggaagtgt tgggcaaacg cctgacgcct ttcgaccgcg ctattgatat gcacatttcc
    32821 aacctgcgtc gtaaactgcc ggatcgtaaa gatggtcacc cgtggtttaa aaccttgcgt
    32881 ggtcgcggct atctgatggt ttctgcttca tgataggcag cttaaccgcg cgcatcttcg
    32941 ccatcttctg gctgacgctg gtgctggtgt tgatgttggt tttgatgtta cccaagctcg
    33001 attcacgcca gatgaccgag cttctggata gcgaacagcg tcaggggctg atgattgagc
    33061 agcatgtcga agcggagctg gcgaacgatc cgcccaacga tttaatgtgg tggcggcgtc
    33121 tgttccgggc gattgataag tgggcaccgc caggacagcg tttgttattg gtgaccaccg
    33181 aaggccgcgt gatcggcgct gaacgcagcg aaatgcagat cattcgtaac tttattggtc
    33241 aggccgataa cgccgatcat ccgcagaaga aaaagtatgg ccgcgtggaa ctggtcggtc
    33301 cgttctccgt gcgtgatggc gaagataatt accaacttta tctgattcgt ccggccagta
    33361 gttctcaatc cgatttcatt aacttattgt ttgaccgccc gctattactg ctgattgtca
    33421 ccatgttggt cagtacgccg ctgctgttgt ggttggcctg gagtctggca aaaccggcgc
    33481 gtaagctgaa aaacgctgcc gatgaagttg cccagggaaa cttacgccag cacccggaac
    33541 tggaagcggg gccacaggaa ttccttgccg caggtgccag ttttaaccag atggtcaccg
    33601 cgctggagcg catgatgacc tctcagcagc gtctgctttc tgatatctct cacgagctgc
    33661 gcaccccgct gacgcgtctg caactgggta cggcgttact gcgccgtcgt agcggtgaaa
    33721 gcaaggaact cgagcgtatt gaaaccgaag cgcaacgtct ggacagcatg atcaacgatc
    33781 tgttggtgat gtcacgtaat cagcaaaaaa acgcgctggt tagcgaaacc atcaaagcca
    33841 accagttgtg gagtgaagtg ctggataacg cggcgttcga agccgagcaa atgggcaagt
    33901 cgttgacagt taacttcccg cctgggccgt ggccgctgta cggcaatccg aacgccctgg
    33961 aaagtgcgct ggaaaacatt gttcgtaatg ctctgcgtta ttcccatacg aagattgaag
    34021 tgggctttgc ggtagataaa gacggtatca ccattacggt ggacgacgat ggtcctggcg
    34081 ttagcccgga agatcgcgaa cagattttcc gtccgttcta tcggaccgat gaagcacgcg
    34141 atcgtgaatc tggcggtaca ggattggggc tggcgattgt tgaaaccgcc attcagcagc
    34201 atcgtggctg ggtgaaggca gaagacagcc cgctgggcgg tttacggctg gtgatttggt
    34261 tgccgctgta taagcggagt taaactccgc atttgtaggc aggataaggc gtttacgccg
    34321 catccggcat ttgagcagga tgcctgatgc gacgctgata gcgtcttatc aggcctacac
    34381 tccgggggtt attttcccca caaccggcgg gaaaaatctt cgatcttgcc gctcagtcgg
    34441 cgcttttgca tcgtccgcgt ccagcttttc gataacccgg cggcggagag taaacggtga
    34501 tactgatcgt catcaaacgg catatgccat gcgatggcgg cagcctcctg cacggtcaca
    34561 tcgctgacac gggaaaccaa ctccagcggc gcgtccgcag acactttccc tggcgctatc
    34621 acgctataca accaccccac cttacctgtg ttttgcatca actgcgctat atcgctgata
    34681 tcaaaatggt aattgagttt atagcaaggc gagcgcggct ggctgacctg aattaatgcc
    34741 tctccccagc ggaaaatatc gcccatatga acattacttt ccgtgagacc gtcggttgag
    34801 agattttcgc caaacgcagg cgcaacaaac aactccgcct gttccggaaa ttcccgcacc
    34861 cagtagagat agtgctcacg aggataatga cacagcgctc tgtctggccc gccgtgaacc
    34921 tttttctccg cctgctcgtc accttccagc cccagctccg tcagcatcaa ctcaccatca
    34981 acctggattt tggcaattgc gctgggtttg ccttcgggat aagcctgaat cttgcctgtg
    35041 tatacatcaa ccggatatcg catctgttcc ctctccacga gaaatttccc cattagacca
    35101 tatttatttt atgtcagccg tgctgttttc gcggtaagta tccaggcagg aactcaatag
    35161 caaaccggac gaacgttagt tcatccggct gtggg
//
LOCUS       DAJTSI010000024        34981 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000024 DAJTSI010000000
VERSION     DAJTSI010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34981
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.024"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..800)
                     /locus_tag="OO327_001084"
     CDS             complement(<1..800)
                     /locus_tag="OO327_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6017711.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQAL"
     gene            892..978
                     /locus_tag="OO327_001085"
                     /pseudo
     CDS             892..978
                     /locus_tag="OO327_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1383..1859)
                     /locus_tag="OO327_001086"
     CDS             complement(1383..1859)
                     /locus_tag="OO327_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR6017712.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1918..3207)
                     /gene="bioA"
                     /locus_tag="OO327_001087"
     CDS             complement(1918..3207)
                     /gene="bioA"
                     /locus_tag="OO327_001087"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR6017713.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAS
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3294..4334
                     /gene="bioB"
                     /locus_tag="OO327_001088"
     CDS             3294..4334
                     /gene="bioB"
                     /locus_tag="OO327_001088"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR6017714.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4331..5485
                     /gene="bioF"
                     /locus_tag="OO327_001089"
     CDS             4331..5485
                     /gene="bioF"
                     /locus_tag="OO327_001089"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR6017715.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5472..6227
                     /gene="bioC"
                     /locus_tag="OO327_001090"
     CDS             5472..6227
                     /gene="bioC"
                     /locus_tag="OO327_001090"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR6017716.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6220..6897
                     /gene="bioD"
                     /locus_tag="OO327_001091"
     CDS             6220..6897
                     /gene="bioD"
                     /locus_tag="OO327_001091"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6017717.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7476..9497
                     /gene="uvrB"
                     /locus_tag="OO327_001092"
     CDS             7476..9497
                     /gene="uvrB"
                     /locus_tag="OO327_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR6017718.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9689..10597)
                     /gene="yvcK"
                     /locus_tag="OO327_001093"
     CDS             complement(9689..10597)
                     /gene="yvcK"
                     /locus_tag="OO327_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR6017719.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10862..11006
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            10994..11983
                     /gene="moaA"
                     /locus_tag="OO327_001094"
     CDS             10994..11983
                     /gene="moaA"
                     /locus_tag="OO327_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR6017720.1"
                     /translation="MASQLTDVFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12005..12517
                     /gene="moaB"
                     /locus_tag="OO327_001095"
     CDS             12005..12517
                     /gene="moaB"
                     /locus_tag="OO327_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR6017721.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12520..13005
                     /gene="moaC"
                     /locus_tag="OO327_001096"
     CDS             12520..13005
                     /gene="moaC"
                     /locus_tag="OO327_001096"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR6017722.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            12998..13243
                     /gene="moaD"
                     /locus_tag="OO327_001097"
     CDS             12998..13243
                     /gene="moaD"
                     /locus_tag="OO327_001097"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR6017723.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13245..13697
                     /gene="moaE"
                     /locus_tag="OO327_001098"
     CDS             13245..13697
                     /gene="moaE"
                     /locus_tag="OO327_001098"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR6017724.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13834..14538
                     /locus_tag="OO327_001099"
     CDS             13834..14538
                     /locus_tag="OO327_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR6017725.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14743..15456
                     /locus_tag="OO327_001100"
     CDS             14743..15456
                     /locus_tag="OO327_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR6017726.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15492..16448)
                     /locus_tag="OO327_001101"
     CDS             complement(15492..16448)
                     /locus_tag="OO327_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCR6017727.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16448..17689)
                     /gene="clsB"
                     /locus_tag="OO327_001102"
     CDS             complement(16448..17689)
                     /gene="clsB"
                     /locus_tag="OO327_001102"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR6017728.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17686..18447)
                     /locus_tag="OO327_001103"
     CDS             complement(17686..18447)
                     /locus_tag="OO327_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6017729.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18580..18990
                     /locus_tag="OO327_001104"
     CDS             18580..18990
                     /locus_tag="OO327_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR6017730.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18952..20058)
                     /locus_tag="OO327_001105"
     CDS             complement(18952..20058)
                     /locus_tag="OO327_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6017731.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20069..21202)
                     /locus_tag="OO327_001106"
     CDS             complement(20069..21202)
                     /locus_tag="OO327_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6017732.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21195..22931)
                     /locus_tag="OO327_001107"
     CDS             complement(21195..22931)
                     /locus_tag="OO327_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6017733.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22924..23919)
                     /gene="hlyD"
                     /locus_tag="OO327_001108"
     CDS             complement(22924..23919)
                     /gene="hlyD"
                     /locus_tag="OO327_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR6017734.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23922..24593)
                     /gene="cecR"
                     /locus_tag="OO327_001109"
     CDS             complement(23922..24593)
                     /gene="cecR"
                     /locus_tag="OO327_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR6017735.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24822..26009
                     /gene="rhlE"
                     /locus_tag="OO327_001110"
                     /pseudo
     CDS             24822..26009
                     /gene="rhlE"
                     /locus_tag="OO327_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="OO327_001111"
     CDS             complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="OO327_001111"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR6017736.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26818..28968
                     /gene="dinG"
                     /locus_tag="OO327_001112"
     CDS             26818..28968
                     /gene="dinG"
                     /locus_tag="OO327_001112"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR6017737.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITVPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28996..29958
                     /gene="ybiB"
                     /locus_tag="OO327_001113"
     CDS             28996..29958
                     /gene="ybiB"
                     /locus_tag="OO327_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR6017738.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30099..31184
                     /gene="hcxB"
                     /locus_tag="OO327_001114"
     CDS             30099..31184
                     /gene="hcxB"
                     /locus_tag="OO327_001114"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR6017739.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(31413..31673)
                     /locus_tag="OO327_001115"
     CDS             complement(31413..31673)
                     /locus_tag="OO327_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6017740.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="OO327_001116"
     CDS             complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="OO327_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR6017741.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="OO327_001117"
     CDS             complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="OO327_001117"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR6017742.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32997..34981)
                     /locus_tag="OO327_001118"
                     /pseudo
     CDS             complement(32997..34981)
                     /locus_tag="OO327_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8289 a         8970 c         9450 g         8272 t
ORIGIN      
        1 tgcagggctt gcagagtgcg agtcgacaat ggattgttat aaatattaac atctgcataa
       61 atgtgcagat tcatgatact gtctggcaat gtgttcagct gattgttagg gatatatagt
      121 gattgcagac gttgtggaag ttcgggcagt ctgtgtagtt ggttttcccc aacgtttaaa
      181 agcctcagat tttgaggaag gggcggtagc tcttgtaatc tgttatcaaa agcacttagt
      241 tcctgtagtt cacatggtaa ttctggtaag gcagtaagct ggttgttgcc aacagaaagt
      301 ttttgtagag ccgggggaag atcaggtagc gtctccaggc tattgagaag ggctgagagt
      361 gactgcaagg aagatggaag agaaggcaaa catgttaaaa ctctgttaag tgaaacatca
      421 agagcgacca ggtgaggcgg aagagccggg agcctgctca gtctgttatc gctggcttta
      481 agtacagtta aggacggagg gagttctggc agagagcgta gctcattgct agagatgtta
      541 agttcttgta tgtgcggggg caggtatggg agagagcgta atctgagtaa acttaaattg
      601 agggctggct cttgaaaagc cagacatatt ttcagtaatc gaactgcctg tgttcggtct
      661 tctgttgcag caccttcctt ggcccagtta tcccagatgc ggtgataatc cgtgatattt
      721 tgctcttcta cggataaacg ggatatacat cctaaaccac aaaaggagcg gaaaaaagaa
      781 ggattgtggg caggtttcat ttattattga tcctcattgt ttatatatta atatattcgt
      841 gcaaatggag tgaggattct ttaaaaaaac gcgcctgtaa atagttaaga acaggaagaa
      901 ctgttgggga tgtggctggc cgaaaatgaa ggggtgaagt tctggctcaa tgtgctgact
      961 gaactgaaaa aacgcggttg gtcgccctac cttttcatca agccagtagt ctgcccacca
     1021 cagcatcatt tctctgcgct tatcgagata ctgagcatgg ttgtaaattc agctaaccca
     1081 gtgcgttgac aaaaaattag cgcaagagga caaaaaatca ccttgcgcta atgctctgta
     1141 tcaggtcact aataccatct aagtggttga ttcatagtga ctggatatgt tgtgttttgc
     1201 agtattatgc agtctatttt ttaagctaaa tgtattataa tgcattgata ttaatgattt
     1261 ttactgtttc tcggtcagct tttttatact aacttgagcg aaacgggaag gtaaaaagac
     1321 aaaaagttgt ttttaatacc tttaagtgat accagatggc attgcgccat ctggcagagt
     1381 gattaactaa acatcgcggt aatcgatgcg cttgccagag agtggaaatg aacgttaaac
     1441 ccgaccatcg cgccgctggc tccttcatcg acatcaatac gctctacatc cagcgcgtga
     1501 acggtaaaaa tgtagcgatg ggtttcgcct ttcggcggcg ctgcgccatc gtatccggtt
     1561 ttaccaaaat cggtacgcgt ctgcaaaacg ccgtctggca ttgctaccag accagagcca
     1621 aacccttgcg gtaatacgcg ggtatcagcg ggtaaattaa caactaccca gtgccaccag
     1681 ccggagccgg ttggcgcatc cgggtcgtag caggtgacaa caaaactttt cgttcccgca
     1741 ggaacatcat cccacgccag atgcggtgaa atattatcgc catcgtaacc catgccgtta
     1801 aagacatgac gatgcggcaa cttatcgcca tcgcgcagat cgttactgat gagtttcatg
     1861 aaccctcctt tcttgtttgc agaaagtgta gccagaaacc ctcacgctga cttcccgtta
     1921 ttggcaaaaa aatgtttcat cctgtaccgc gcggttaacc gctgcggtca gacgctgcaa
     1981 ctgttgcggg agaataatat agggcggcat caggtaaatc agtttgccaa aaggccggat
     2041 ccataccccc tgttcgacaa agaatttttg cagcgccgcc atattcaccg gacgagtggt
     2101 ttcgaccaca ccgattgccc ccagtacgcg cacatcggca accatttcgg catcacgggc
     2161 tggtgccagt tgctcgcgca gctgcacttc aatagccgcc acctgctgct gccattcgcc
     2221 ggattcgata atcgctaggc tggcgtttgc tgccgcgcag gccagcggat tgcccataaa
     2281 agttggccca tgcataaagc agccggcttc gccgttactg atggtttctg caacctcgcg
     2341 cgtggtaagt gtggcggaaa gggtcattgt gccgccggtt aaggctttac cgaggcacaa
     2401 aatgtccggc gcgatttctg catattcaca ggcaaacagt ttgccggtac gaccaaatcc
     2461 ggtggcgatc tcgtcggcaa tcagcaagat accttcgcga tcgcacattt tgcggattcg
     2521 ttttaaccat tccggatggt acatgcgcat cccgcttgcg ccctggacaa tcggctcaat
     2581 gatcaccgct gcgatttcat gacgatgcgc cgccatcagg cgggcaaaac caaccatatc
     2641 gcgttcatcc cattcaccat ccatgcggct ttgcggggcg ggagcaaaca agttttctgg
     2701 cagatagcct ttccacaggc tgtgcattga gttgtccgga tcgcacaccg acatcgcgcc
     2761 aaaggtatcg ccatgataac cattgcggaa ggtcagaaaa cgctggcgcg cttcgccttt
     2821 ggcttgccag tactgcaacg ccattttcat cgccacttcc accgctacgg aaccggagtc
     2881 cgcgagaaaa acgcactcca gcggttgcgg cgtcattgcc accagtttgc ggcatagctc
     2941 gatggcgggc gcatgggtga taccgccaaa catcacatgc gacatggcat caatttgcgt
     3001 cttcatcgcc gcattaagct gcgggtgatt gtagccgtgg atcgccgccc accaggacga
     3061 cataccgtca accaggcgtc tgccgtcaga caaaattagc tcgcaacctt cggcgctcgc
     3121 caccggataa accggcagag gggaggtcat ggatgtgtat gggtgccaga tatggcgttg
     3181 gtcaaaggca agatcgtccg ttgtcataat cgacttgtaa accaaattga aaagatttag
     3241 gtttacaagc ctacaccgaa ttaacaacaa aaaacacgtt ttggagaagc cccatggctc
     3301 accgcccacg ctggacattg tcgcaagtca cagaattatt tgaaaaaccg ttgctggatc
     3361 tgctgtttga agcgcagcag gtgcatcgcc agcatttcga acctcgtcag gtgcaggtca
     3421 gcacgttgct gtcgattaag accggagctt gtccggaaga ttgcaaatac tgcccgcaaa
     3481 gctcgcgcta caaaaccggg ctggaagccg agcggttgat ggaagttgaa caggtgctgg
     3541 agtcggcgcg caaagcgaaa gcggcaggat cgacgcgctt ctgtatgggc gcggcatgga
     3601 agaatcccca cgaacgcgat atgccgtacc tggaacaaat ggtgcagggg gtaaaagcga
     3661 tggggctgga ggcgtgtatg acgctgggca cgttgagtga atctcaggcg cagcgcctcg
     3721 cgaacgccgg gctggattac tacaaccaca acctggacac ctcgccggag ttttacggca
     3781 atatcatcac cacacgcact tatcaggaac gcctcgatac gctggaaaaa gtgcgcgatg
     3841 ccgggatcaa agtctgctca ggtggcattg tgggcttagg cgaaacggta aaagatcgcg
     3901 ccggattatt gctgcaactg gcaaacctgc cgacgccgcc ggaaagcgta ccaatcaaca
     3961 tgctggtgaa ggtgaaaggc acgccgcttg ccgataacga tgacgttgat gcctttgatt
     4021 ttattcgcac cattgccgta gcgcggatca tgatgccgac ctcttacgtg cgcctttctg
     4081 ccggacgcga gcagatgaac gaacagactc aggcgatgtg ctttatggca ggcgcaaact
     4141 cgattttcta cggttgcaaa ctgctgacca cgccgaatcc ggaagaagat aaagacctgc
     4201 aactgttccg caaactgggg ctaaatccgc agcaaactgc cgtgctggca ggggataacg
     4261 aacaacagca acgtcttgga caggcgctga tgaccccgga caccgacgaa tattacaacg
     4321 cggcagcatt atgagctggc aggagaaaat caacgcggcg ctcgatgcgc ggcgtgctgc
     4381 cgatgccctg cgtcgccgtt atccggtggc gcaaggagcc ggacgctggc tggtggcgga
     4441 tgatcgccag tatctgaact tttccagtaa cgattattta ggtttaagcc atcatccgca
     4501 aattatccgt gcctggaagc agggggcgga gcaatttggc atcggtagcg gcggctccgg
     4561 tcacgtcagc ggttatagcg tggtgcatca ggcgctggaa gaagaactgg ccgagtggct
     4621 tggctattcg cgggcgctgc tgtttatctc tggttttgcc gcgaatcagg cagtcattgc
     4681 cgcgatgatg gcgaaagagg accgtattgt tgccgaccgg cttagccatg cctcattgct
     4741 ggaggctgcc agtttaagcc cgtcgcagct tcgccgtttt actcataacg atgtcgctca
     4801 tctggcgcga ctgcttgctt ccccctgtcc ggggcagcaa ctggtggtga cagaaggcgt
     4861 gttcagcatg gacggcgata gcgcgccact ggcggaaatc cagcaggtaa cgcaacagca
     4921 caatggctgg ttgatggtcg atgatgccca cggcacgggc gttatcgggg agcaggggcg
     4981 cggcagctgc tggctgcaaa aggtaaaacc agaattgctg gtagtgactt ttggcaaagg
     5041 atttggcgtc agtggggctg cggtgctttg ctcaagtacg gtggcggatt atctgctgca
     5101 attcgcccgc caccttatct acagcaccag tatgccgccc gctcaggcgc aggcattacg
     5161 tgcgtcgctg gcggtcattc gccgtgatga gggggatgca cggcgcgaaa aactggtgtc
     5221 actcattgcg cgttttcgtg ccggagtaca ggatttgccg tttacgctgg ctgattcatg
     5281 cagcgccatc cagccattga tcgtcggtga taacagccgt gcgttacaac tggcagaaaa
     5341 actgcgccag caaggctgct gggtcacggc gattcgcccg ccaaccgtac ccgctggtac
     5401 tgcgcgactg cgcttaacgc taaccgctgc gcatgaaatg caggatatcg accgtctgct
     5461 ggaggtgctg catggcaacg gttaataaac aagccattgc agcggcattt ggtcgggcag
     5521 ccgcacacta tgagcaacat gcagatctac agcgccagag tgctgacgcc ttactggcaa
     5581 tgcttccaca gcgtaaatac acccacgtac tggacgcggg ttgtggacct ggctggatga
     5641 gccgccactg gcgggaacgt cacgcgcagg tgacggcgtt agatctctcg ccgccaatgc
     5701 ttgcgcaagc ccgccataaa gatgccgcag atcattatct ggcaggagat atcgaatccc
     5761 tgccgttagc gactgcgacg ttcgatcttg catggagcaa tctcgcggtg cagtggtgcg
     5821 gtaatttatc cacggcactc cgcgagctgt atcgggtggt gcgccccgga ggcgtggtcg
     5881 cgtttaccac gctggtgcag ggatcgttac ccgaactgca tcaggcgcgg caggcggtgg
     5941 acgagcgtcc gcatgctaat cgctttttac cgccagatga aatcgaagag tcgctgaacg
     6001 gcgtgcgtta tcaacatcat attcagctca tcacactatg gtttgatgat gcgctcagtg
     6061 ccatgcgttc gctgaaaggc atcggtgcta cgcatcttca tgaagggcgc gacccgcgaa
     6121 tattaacgcg ttcgcagttg cagcgattgc aactggcctg gccgcaacaa caggggcgat
     6181 atcctctgac gtatcatctt tttttgggag tgattgctcg tgagtaaacg ttattttgtc
     6241 accggaacgg ataccgaagt ggggaaaacc gtcgccagtt gtgcactttt acaagccgcg
     6301 aaggcagtag gttatcggac ggcaggttat aaaccggtcg cctctggcag cgaaaagacc
     6361 ccggaaggct tacgtaatag tgacgcgctg gcgttacagc acaacagcag cctgcagctg
     6421 gattacgcaa cggtaaatcc ttacaccttc gcagaatcca cctcgccgca catcatcagc
     6481 gcccaagagg gcaggccgat agaatcattg gtaatgagcg ccggattacg cgcgcttgaa
     6541 caacaggctg actgggtgtt agtggaaggt gctggcggct ggtttacgcc gctttctgac
     6601 actttcactt ttgcagattg ggtaacacag gaacaactgc cggtgatact ggtagttggt
     6661 gtgaaactcg gctgtattaa tcacgcgatg ttgactgcgc aggcaataca acacgccgga
     6721 ctgactctgg cgggttgggt ggcgaacgat gttacgcctc cgggaaaacg tcacgctgaa
     6781 tatatgacca cgctcacccg catgattccc gcgccgctgc tgggagagat cccctggctt
     6841 gcagaaaatc cagaaaatgc ggcaaccgga aagtacataa accttgcctt gttgtagcca
     6901 ttctgtattt ggttaaattg cgagcgagat cgcgtcttcg attgactgca atttaaccaa
     6961 ttaaattcta aaataatcac gaaaaaaatt ttacttccgc ctcatgcggc gaatgtggga
     7021 attgcccagg cggcggggga taggggctgg agacagttat ccactattcc tgtggataac
     7081 catgtgtatt agagttagaa aacacgaggc aagcgagaga atacgcggct tgcacgcgaa
     7141 ttggcgttaa agacggctca aagaaatatc ttttattttt taactggtta gataaatgca
     7201 atggcagtca ctgaacaggc atctcttgcc ataaaactgt catcactcat cttgacaaat
     7261 gttaaaaaag ccgttgcttt ggggataacc cggtaaggct ggagttttat ctcgccacag
     7321 agtaaatttt gctcatgatt gacagcggag tttacgctgt atcagaaata ttatggtgat
     7381 gaactgtttt tttatccagt ataatttgtt ggcataatta agtacgacga gtaaaattac
     7441 atacctgccc gcccaactcc ttcaggtagc gactcatgag taaaccgttc aaactgaatt
     7501 ccgcttttaa accttctggc gatcagccag aggcgattcg acgtctcgaa gaggggctgg
     7561 aagatggcct ggcgcaccag acgttacttg gcgtgactgg ctccgggaaa accttcacca
     7621 ttgccaatgt cattgctgac cttcagcgcc caaccatggt acttgcgccc aacaaaacgc
     7681 tggcggccca gctgtatggc gaaatgaaag agttcttccc ggaaaacgcg gtggagtatt
     7741 tcgtctccta ctacgactac tatcagccgg aagcctatgt accgagttcc gacaccttca
     7801 ttgagaaaga tgcctcggtt aacgaacata ttgagcagat gcgtttgtcc gccaccaaag
     7861 cgatgctgga gcggcgtgat gtgattgtag tggcgtctgt ttccgcgatt tatggtctgg
     7921 gcgatcctga tttatatctc aagatgatgc tccatctcac ggtcggtatg attatcgatc
     7981 agcgcgcgat tctgcgccga ctggcggagc tgcaatacgc tcgtaatgat caagcattcc
     8041 agcgtggtac tttccgcgtt cgtggcgagg tgatagatat cttcccggca gaatcggatg
     8101 acattgcact tcgcgtggaa ctgtttgacg aggaagtgga acgattgtcg ttatttgacc
     8161 cgctgaccgg gcagattgtt tccactattc cacgttttac catctacccg aaaacgcact
     8221 acgtcacacc gcgcgagcgc atcgtacagg cgatggagga gatcaaagaa gaactggccg
     8281 ccagacgcaa agtgctgttg gaaaacaaca aactgctgga agagcagcgg ctgacccagc
     8341 gtacccagtt tgatctggag atgatggacg agctgggcta ctgttcgggg attgaaaact
     8401 actcgcgctt cctctccggt cgtggaccgg gtgagccacc gccgacgctg tttgattacc
     8461 tgcctgccga tgggctgctg gtcgtcgatg aatctcacgt caccattcca caaattggcg
     8521 gcatgtatcg cggtgaccgg gcgcgtaaag agacactggt ggagtacggc ttccgcctgc
     8581 catcagcgct ggataaccgt ccgcttaagt ttgaagagtt cgaagcatta gcgccgcaaa
     8641 ccatctatgt ttcggcgacg ccgggtaatt acgagctgga aaaatccggc ggcgatgtgg
     8701 tggatcaggt ggtgcgtcca accggattgc ttgacccgat tatcgaagtg cggccggtgg
     8761 cgacacaggt tgatgatctt ctttcggaga ttcgtcagcg agcggcaatt aacgaacgcg
     8821 tgctggtcac cacactgacc aagcgaatgg cggaagatct taccgaatat ctcgaagaac
     8881 acggtgagcg cgtgcgttat cttcactcag atatcgacac cgtcgagcgt atggagatta
     8941 tccgcgactt gcgtctgggt gagttcgacg tattggtagg gatcaactta ctgcgtgaag
     9001 gtctggatat gccggaagtg tcgctggtgg cgatcctcga cgctgacaaa gaaggcttcc
     9061 tgcgttccga acgttcgttg atccagacca ttggtcgtgc ggcacgtaac gttaacggta
     9121 aagcgattct ctacggcgat aggagcactc catcaatggc gaaagcgatt ggcgaaaccg
     9181 aacgtcgccg tgagaaacag cagaagtaca acgaggaaca cggcattacg ccgcaaggct
     9241 tgaacaagaa agtggtcgat atcctggcgc tggggcagaa cattgccaaa accaaagcga
     9301 agggcagagg aaaatcgcgc ccgattgttg agccggataa tgtgccgatg gatatgttgc
     9361 cgaaagcgtt gcagcagaaa atccatgaac tggaagggct gatgatgcaa cacgcgcaga
     9421 atctggagtt cgaagaagcg gcacaaattc gtgaccagtt gcatcagttg cgtgagttgt
     9481 ttattgccgc atcgtaacag gatagcgaag aagactgatg acaaacggaa aacagtctga
     9541 tgcgctacgc ttatcaggcc tacattttct ccgcaatata ttgaatttgc gcggtttgta
     9601 ggccggataa ggcgttcacg ccgcatccgg cataaacaac gcgcactttg tcttcaatag
     9661 gaagccggaa ttttccttcg gatttccgtt aacctaaagc ctgtaacgcc ttttccagcg
     9721 cgttatgtaa caactggcgg tcatgacgat acggaatatc gctggcctcc agtacctcct
     9781 ggatcacaat ccgctctttc accgccgaga catccacttt tggcccgacg atgaccgcat
     9841 caatgacctt tttaccaaca tactgctcca taattgccag cttgctttcc agcttcaaat
     9901 tagccgcagg taaactcaac tcacgcccca gattgccgat ataaaccatc ggcgctggcg
     9961 tgcggcgtaa tgcctgggcg atttccttca gcagcagaat tggcatcagg ctggtataaa
    10021 aactgccagg cccaataatg atgagatccg cttcattgat agcgtgaacc gcctcacgcg
    10081 ttgcgggtac attaggcgtt aacaataact cttgaatcgg cgtagttaac tggtcgatat
    10141 tgacctcgcc gtaaacttca tgcccctgat cgtcaatcgc catcagatca acaggatgct
    10201 ctgacattgg aatcaaatgc gtatccactt tcagcagatt acgaattaaa ttgatggctt
    10261 ccagaggccg cacgctaagg tgatccagcg cctttaacat caagtttccg agattatgac
    10321 cggaaagttc gccattgccg ccaaaacggt attcaaacat cgcggaggcg acgttcggtt
    10381 ccgttatcag ctggttgagg cagttgcgca tatcgcccca agcaatgccg ccttctgaac
    10441 ggcgaatacg ccccgtcgag ccaccattat ccgtggtggt gacgataccc gttaaacgag
    10501 aacccaaaga cgaaagtgat gagagaacgc gtcccagtcc atgccctccg ccgagagcaa
    10561 cgacacgatc aagatcagcc agcgtacgat tgcgcatata gtttcctgga gtcagattat
    10621 ccgcgctaca gtagcgcaaa ttgcggggaa acagcaatta acctgccaat attgaggata
    10681 caaactattt tcttctatct ctaaaggacg atgcacgcta tgcctccctg atgatgtata
    10741 tcaaagtaaa accgccattt tcccttattc tgtagcgaaa tagcacgatc atgacgctat
    10801 atacatgatt acatagcgaa agtgtggatg gtaaaaatct catttacacg ctagtatcgg
    10861 cttaaccact aaacactcta gcctctgcac ctgggtcaac tgatacggtg ctttggccgt
    10921 gacaatgctc gtaaagattg ccaccagggc gaaggaagaa atgacttcgc ctcccgtatc
    10981 tggaaaggtg tacatggctt cacaactgac tgatgtattt gcgcgtaagt tttactactt
    11041 gcgcctgtcg attaccgatg tgtgtaactt tcgttgcacc tactgcctgc cggatggcta
    11101 caaaccgagc ggcgtcacca ataaaggctt tcttaccgtc gatgaaattc gccgggttac
    11161 gcgcgccttc gccagtctgg gcaccgaaaa agtgcgcctg acaggaggag agccgtcttt
    11221 acgccgcgac tttaccgata tcatcgccgc tgtgcgggaa aacgacgcta tccgccagat
    11281 tgcggtcacc accaatggtt accgtctgga acgcgatgtg gcgaactggc gcgatgcggg
    11341 acttactggc attaacgtca gtgtcgacag tttggacgcc cgccagtttc acgctattac
    11401 cgggcaggat aaattcaacc aggtcatggc agggattgat gctgcatttg aggccggttt
    11461 tgagaaggtc aaagtcaata ccgtgctgat gcgtgatgtt aatcatcacc agctcgacac
    11521 ctttctgaac tggatccagc atcgccctat ccagctgcgt ttcatcgaac tgatggaaac
    11581 gggcgagggc agcgagctct tccgtaagca tcacatctct ggtcaggttc tgcgtgacga
    11641 gctactgcgt cgcggctgga tccaccaatt acgtcaacgc agcgacggtc ccgcgcaagt
    11701 cttttgccat ccagattacg ccggagagat tggccttatc atgccgtatg aaaaagactt
    11761 ctgcgccact tgcaaccgcc tgcgcgtttc ctccattggt aaactccatc tctgcctgtt
    11821 tggtgaaggc ggcgttaacc tgcgcgatct gctggaaggc gatacccagc aacaggcgct
    11881 ggaagcgcgt atttcagcgg cgctgcggga gaagaaacag acccatttcc tgcatcaaaa
    11941 caacaccggt attacgcaaa acttatcgta cattggcggc taaaacgtca aaaggagaga
    12001 tcagatgagt caggtaagca ctgaatttat cccgacccgt attgctattc ttacggtttc
    12061 taatcgtcgc ggtgaagaag acgatacctc cggtcactat ctgcgcgatt cggcgcaaga
    12121 agcgggccat cacgttgtcg atgaagccat tgtgaaagaa aaccgctacg ctattcgcgc
    12181 tcaggtatct gcgtggatcg ccagcgacga tgtacaagtg gtattgatta cgggtggtac
    12241 tggcctgacg gaaggtgatc aggctcccga agcgctgctg ccgttgttcg accgtgaggt
    12301 tgaaggtttt ggtgaagtgt tccgtatgtt gtcgtttgaa gagattggca cttccacgtt
    12361 gcaatctcgt gcggtagcgg gcgtcgctaa caaaacgctg attttcgcca tgccgggttc
    12421 gaccaaagcg tgccgtaccg catgggaaaa tatcatcgcg ccgcagctgg atgcccgtac
    12481 gcgtccgtgt aatttccatc cacatttgaa gaaataagta tgtcgcaact gacccatatc
    12541 aacgccgctg gcgaagcgca catggtggat gtctccgcca aagcggaaac cgtgcgtgaa
    12601 gcgcgggcgg aagcctttgt caccatgcgc agcgagacgc tggcgatgat tattgatggt
    12661 cgccaccaca aaggcgacgt atttgccact gcgcgtattg ccggtattca ggcggcaaaa
    12721 cgcacttggg atctgatccc gctgtgtcac ccgctgatgc tcagcaaagt tgaagtcaat
    12781 ttacaggccg agccggagca caatcgggtg cgtatagaaa ccttatgccg cctgaccggg
    12841 aaaaccggtg tcgaaatgga agcgttaacc gcggcctccg tggcggcgct gaccatttat
    12901 gacatgtgca aagcggtgca aaaagatatg gtgattggtc cggtacgttt gctggcgaag
    12961 agcggcggca agtcgggtga ctttaaggtg gaagcggatg attaaagttc tttttttcgc
    13021 ccaggtgcgc gagttggtgg gaacagatgc aaccgaagtg gctgcggatt acccaaccgt
    13081 tgaagcgtta cgccagcacc tggctgcgca gagcgatcgc tgggcgctgg cgctggaaga
    13141 tggcaaattg ctggctgccg tcaaccagac gctggtgagt tttgaccatc cgctgactga
    13201 cggcgacgaa gtagctttct tcccgccggt aaccggaggt taagatggca gaaaccaaaa
    13261 ttgttgtcgg cccgcagccg ttcagcgtag gagaagagta cccgtggctg gcggagcgtg
    13321 acgaagacgg tgcggtagtc accttcaccg gaaaggtgcg caaccataac ctgggcgaca
    13381 gcgtcaaagc attaaccctc gaacactatc cggggatgac tgaaaaagca ctggcagaaa
    13441 ttgttgatga agcgcgtaac cgctggccgc tggggcgcgt cacggtgatt caccgcatcg
    13501 gggaattatg gccgggcgat gaaatcgttt ttgtcggtgt caccagtgcg catcgcagca
    13561 gtgcgtttga agccgggcag tttattatgg attatctcaa aacccgcgca ccgttctgga
    13621 agcgcgaagc cacgccggaa ggcgaccgct gggttgaagc ccgggagagc gatctgcagg
    13681 cggcaaaacg ctggtagttt tttgttagcc ggataaggca ccgctgcgtc cggcaatttc
    13741 accgctcagt acatgcgttt acgctgcgtc gatgcacagc ctcatcattt tgcagtatcc
    13801 ttaagatatt ccttatatct tcaggagatc gtcatggaca gattcccacg ttctgattca
    13861 atcgtacaac cctgggctgg cttgcaaact tatatggcgc aagtctatgg ctggatgacc
    13921 gttggcttgt tgctgaccgc atttgttgcc tggtatgcgg ctaattccgc ggccgtgatg
    13981 gagctgttgt tcactaaccg tgtcttttta atcggtctga tcatcgcgca attagcattg
    14041 gttattgtgt tatcagcgat gattcaaaag ctgagcgcag gtgtaacgac gatgctcttt
    14101 atgctttatt cggcgctgac gggtcttacg ctttccagta tattcattgt ctataccgct
    14161 gcttctatcg ccagtacttt cgtcgttact gccgggatgt tcggcgcaat gagcctgtac
    14221 ggttacacca cgaagcgcga tttaagtggc tttggcaata tgctgtttat ggcgttaatc
    14281 ggcattgtgc tggcatcgct ggtcaacttc tggctgaaaa gcgaagcatt gatgtgggca
    14341 gttacctaca tcggcgtgat tgtctttgtc ggattgacgg cgtatgacac tcagaaattg
    14401 aaaaatatgg gtgagcagat tgatacccgc gacacgtcga acctgcgcaa atattccatt
    14461 cttggtgcgt taaccttgta tctggacttc atcaacctgt tcctgatgtt gttacggatc
    14521 ttcggcaacc gccgttaatt ctttttcacc atcactatcc cggccatgtg tcgggatttt
    14581 tttcttacta aaaacgacac ttaacaagta ccaggtataa aatatgcctt atgagcgagc
    14641 ggaatcgttt tccgtatcca taaatacaaa agtattttgt atgcgtggtt ttaaatttat
    14701 tgatggtgaa tattaatgtt ttttctaagg acaactaaaa gaatggaatc atactcgcaa
    14761 aacagcaata aattagattt tcagcacgag gccaggatat taaacggtat atggctcatt
    14821 accgctttag gtttggtggc aaccgcagga ctagcctggg gagctaagta tctcgaaatt
    14881 acggcaacca aatatgattc accaccaatg tatgtcgcca tagggttatt attactttgt
    14941 atgtatggct taagtaagga tatcaacaag ataaatgccg ccatcgcggg cgtaatatat
    15001 ctgtttttac tctctttggt ggcgattgtc gttgcaagtt tagttcctgt atatgccatt
    15061 atcatcgtgt tcagcactgc gggcgcgatg tttttaatca gtatgctggc cggtttatta
    15121 tttaatgttg atcctggttc tcaccgtttt atcattatga tgacgttgac agggttggcc
    15181 ctggtaatca tcgtgaatgc ggcattaatg agtgaacggc ccatttgggt aataagttgc
    15241 ttaatgattg tgttatggcc aggcattatc tcgcatggac gaaataagct ccttgaactg
    15301 gcaggaaaat gccatagtga agagttgtgg agtccggttc gttgcgcttt tacaggtgca
    15361 ttaacactct attactattt tatcggcttc tttgggatac ttgccgcgat agctataacg
    15421 cttgtctggc aaaggcatac gcgttttttt cattagtcgt aaatgcagat actaaagaac
    15481 ttaactaaac ttcacatcgc cgcttcattt ttcgcccgta gcttcttcgc ttggctttcc
    15541 agtagcaggt aacagatcag cgccagcagc agcgggataa agtaatacag gacgcgataa
    15601 gcgagcaggg cggcgataat gctccctttc gaggtatgct ccccagccag tagtgcgata
    15661 aacaccgctt ccagcacacc gatccccgcc ggaatatgca cgatgacgcc agcaatacta
    15721 ctaaccagta acacgcccag tacaaagaaa tagttcacgc tttgaccaag taacagccag
    15781 ataatcgccc ccattaccat ccagttaacg ctggaaatca gcatctgagc gagggcgaat
    15841 ttccatgaag gcagcaccag cttttgccct ttgatggtca tatggcggtg cttcgcgaaa
    15901 gcgcaaaacc acaaataaac cgcgataatc atcagtaagc caatgccgag aatacgcagc
    15961 gtagtttgat cgacatacca gtaatccggc aactccacca cgcctgcggt aaagataatc
    16021 cctgccagta aaatatagcc cagccagttg gtggtaatac tgagcgagga aatccgcgta
    16081 atagtgctgc ccggtaaccc tagtcgggag tacaaacgat agcgcatacc aatgccgccg
    16141 acccaggtac tgagcgtcag gttgaaggcg tagcagataa acgacaccag catcacctgg
    16201 cgcttcgcca gtttgtgacc gcagtaaaag cgggcgagca ggtcatagca gccgtaaatc
    16261 agatagctga cgaccaccag cccgaccgca ctaagcagcg caacgcgatt gtagtcgcgg
    16321 atgaccttcc agacctcttc ccagtccact tttttggcgt agaccaccag caacacaatc
    16381 accgcgataa aaaacagcca ggtgaggatc ttctttgcta agcgccagcg cgggtgtgat
    16441 ttactcatca ggggtttacc cccgtgtttt cagtttctac ccgatcctgc gtttccattg
    16501 tcggttgtgc ggggggatca acctgcgtca gacgtggtgt gtgtgccgga agccagccaa
    16561 ccagcgccgg gaagtggcgt aaaaagtgga acgccagcac gcttttggtc aggttccacc
    16621 aggtgcgttt gggcagcatg gtttcatcca cctgctgaca atctgcggca ataatgccgt
    16681 tcagattatc gcgcagcgtc tggttaaaat gacgatcgtg gatgatgaca tttgcttcga
    16741 gattcagtga caaactgagc ggatcgagat tactggaccc tactgtcgcc cagtgatcgt
    16801 ccatcaatgc cactttgccg tggagcgggc ggcggcggta ctcaaaaacc tgaacgccgc
    16861 ctttaaccag atagttatac agcaagcgcg caccgactct gacaatcggc atatccggtt
    16921 cgccctgaat gatcagtttg atccgcaccc cgcgccgtgc cgctttacgc aaggcgtgta
    16981 aaaatcgata gccggggaag aagtaggcgt tggcgataat cacttcccgc tgcgcctgag
    17041 tgagcatttt cagataatgg cgttcaatat catcgcgatg ttcttcgtta tcgcgccaga
    17101 ccagcaatac ctgcgcttct cccggctggc ggttctcttc cgctttgtga tgacgtcgcc
    17161 accagcgtcg tgccgcgctc tgtccaggca ggttttccag ctcaaactgg agaatatctt
    17221 cgacaatcgg cccttcaagg cgtaccgcgt aatcctgttt agcctctgga ccgtagctgg
    17281 acatatgctc ggcggagtaa ttcagcccgc caataaaggc gatacgcgcg tcgatcacca
    17341 caattttgcg atgcatccgg cgaaacacat tggtgcgcat accaaaaagg cgagggcggg
    17401 gatcgtagta gcggaacact acgccagctg ccgtcagttc attgacaaac tcatcgctga
    17461 gatccggcga accgtagcca tccagcaaga cttccgcttt aaccccgcgt tgcgctgctg
    17521 ccagtagtgc cgcatgcagc tgtttgccga cgttatcctc aaaccagata aacgtttcaa
    17581 gaatgatgcg ttcttgtgcc tcgccaatcg ccttaaacac cgcgggataa tattgctcgc
    17641 cgttttccag caactggatc ttattgcctt cgcgccagct acatttcata aatgaatctc
    17701 cgcacttaaa ggggcatgat cagaaaggtg tcgccatgtc cgcagcggca acgcggttgg
    17761 cgcgctggcg ctggcatttt tgacgtagat cctgtccagt cgtagtagag gaaattgcac
    17821 cggaaacgtg cgcgccgggc gtccgtgggc gcgggtaaaa atctcatcca gtccggcctg
    17881 cacttttaac ggatgattag ctttttgccg ccagtcattg aaatcacccg ccaccaatac
    17941 cggttcgccg tccggtagct cattcaccca ttcggcgagc atcgcaagct gcgcctgacg
    18001 gtgcgcctca cgcaggccca gatgtacgca catcacatga atcgcttttc cggtcatcgg
    18061 cggcacaatg cggcagtaga gcacgccgcg cttttccgca ccatcgaccg aaacatcgcg
    18121 attctcataa tgttcaatgg gataacgcga cagtacggcg ttgccgtgat gcccttccgg
    18181 gtatacggca ttgcgaccgt aggcaaaatc gctccacata gtgtcggcga gaaactcgta
    18241 gtgcgaggta tcgggccagt tttccacatg cagcggatga acttcgtgcg cgcccatcac
    18301 ttcctgcagg caaacaatat cggcgctgac ggtacgcacg gcatcgcgaa gttccggcaa
    18361 aatgaagcgt cggttaaacg cggtaaagcc tttgtgaatg ttgatggtga gcaccttgaa
    18421 cgaaaattgt tgtgtttgat cgggcataat tttcctgtct ttgcctctta tctcattgaa
    18481 atagtgtagt cggcgtcaca aaaaggtgcg gtcttacgga attttccgta aagttcggta
    18541 ctctgagtaa gtagagataa attcttcagg agagaagcca tgaagtggca acaacgtgtt
    18601 cgtgtcgcaa cgggtctaag ttgctggcag attatgttgc atttactggt agtggcgctg
    18661 ctggtggtgg gctggatgag taagactctg gttcacgtcg gcgtgggatt atgcgcactg
    18721 tattgtgtca cggtagtgat gatgctggtg tttcagcgcc accccgagca acgctggcgt
    18781 gaggtggcag acgtgctgga agagctgacc acgacctggt attttggcgc agcgctgatt
    18841 gtgctgtggc tgttgtcccg cgtactggaa aacaactttt tgctggcaat tgcagggctg
    18901 gcgatccttg ctggcccggc ggtagtgtct ttgctggcga aagataagaa gttacatcac
    18961 cttacgtcta aacatcgcgt acgccgctga ccctgtcgtg gccgttatca ccagtagcgg
    19021 ccacaaacta ttccacacaa tatccagact cgcatccttc aaataaatct gcttggtaat
    19081 gtccgtaaag tggcgaatag ggttaatcca cgtcagattt tgcagccaca ctggcatgtt
    19141 ttccaccggc gaaacgtaac cggaaaggag aatggcgggc atcataaaaa caaacacgcc
    19201 gataaacgcc tgctgttgtg tcgagcagag tgatgaaatc aacagaccga atcccaccag
    19261 cgataaaccg taaatcacca tcgtaaagta gaacagcgct agcgatccgg cgaaggggat
    19321 ttgatacgcc cagataccaa tcgccagcac aatggtggcc tggaaggtgg cgacaattaa
    19381 cgccggtacg gctttgccga tgaagatctg ccaggtggtg agcggcgaaa ccagtagctg
    19441 atcgagcgta ccttgttcac gttcgcgggc gacggaaagt gaagtgacga tcattacgcc
    19501 gatagtggtg atcatggcga tcagcgacgg caccacaaac catttgtagt cgagattcgg
    19561 gttataccag ttgcgtacca ccagctcgct gttgttgggt ttcggttttc cttccagcag
    19621 ctcctgctga taatttttga cgatctgttg caggtagttg gcggcaattt gcgcactgtt
    19681 ggagttacgc ccgtcgagga tcaactgcaa cggcgcggtc tggaaggtat ccagtttgcg
    19741 cgagaagtca gccgggaaac gcaccagtag taacgccttt tgtgtgtcga tggtcgggcg
    19801 gatctcctgt gggcttttca gcagcagcac atgtgtaaag gcgctggcgc gggcaaaacg
    19861 ttgagtaagt tccaccgaat gctcgccgtt atcttcatcg tagatggcga tggtggcgtt
    19921 agtcacttcc agcgtggcgg cgaacgggaa caggatcacc tgaattagca cgggtaaaat
    19981 cagaatcgcg cgggtttgtg gttcgcgcag caacgactgc aactctttgc ggattaacgt
    20041 ccataagcga tgaaacatgc tcttctccct aatccagccg acgtttggtt ttcagccacg
    20101 tcaggccgat aaacatcacc gccgaagcga tcaaaaacag cacgtttacc accagcacca
    20161 ctggaatatt cccggcgagg aacaggcttt gcagggtgct gacgaaataa cgagcgggaa
    20221 taatgtacgt caccgcgcgg atcaccgcgg gcatactgtc gatctgaaaa ataaagccgg
    20281 aaagcataat cgacggcaga aaagcggcgt tcagggcgac ctgagcggca ttgaactggt
    20341 tgcgggtaat cgtggaaatc agcagcccca ttcccagggt actgagtaaa aacaggctgg
    20401 agataaaaaa cagaatcagc agcgaaccgc gatacggcac gccgagaata aacactgaca
    20461 ccagcataca cagcaacatc gccagcatcc cgagaaagta ataagggatc agcttacaca
    20521 gcagcagttc cgtgcgggta atctccgtag agagcagagc ctccatggtg ccgcgttccc
    20581 attctcgcgc caccaccagc gaggtgagaa tcgcgccgat gaccgtcatg ataatggtca
    20641 ccgcacccgg gataataaag tgctggctta tcgccgccgg gttaaaccag tagcgggttt
    20701 gtacatcaat aagcggttca aaagtctgcc cgttgtcctc cgctcgctgc atttgccaga
    20761 tctgccagat cccttcgaca tacccctgca caaagttagc ggtattcggc tcactgccgt
    20821 cggtgatcac ctgaatcggt gcggtggcgt tggcgcgctc catctgttcg gcgaaatcca
    20881 cgggaataac caccagaccg cgaattttcc ccgactgcat tttggcgatc agttcctgac
    20941 ggttatcgct gatggtggcg tcgatgtagg gcgaaccggt catggtgtgg gtgaaatcca
    21001 gcgccgcttc gctacgctgt tccagcaaaa tcccgacccg cagcttgctg gagtcgaggt
    21061 taatgccgta accaaaaata aacagcagta gcagtgggat cactaccgca atcagccagc
    21121 tactcggatc gcgaacgatc tgccgcgtct ctttgacgca cagcgcccgc acgcgatgcc
    21181 aggaaaggat cgggttactc attgctatgc tccttatccc agtcgtggat caactgaata
    21241 aaggcttgct ccatggtggg atcgggttgc tcatcgttag ctgactgtgc tttcaaatcg
    21301 tccggcgtac cgctggcgat taatttccca cggtacacca ggccgatgcg gtcgcaatat
    21361 tccgcttcat ccataaagtg ggtggtgacc atcaccgtga cgcctttctc taccatgctg
    21421 ttgatgtgca gccaaaattc acggcgggtg agggggtcaa cgccggaagt cggttcgtcg
    21481 agaaagagaa tatccggttc atgcatcagc gaacaggcca gcgccagccg ctgtttaaaa
    21541 cctaatggca gttcatcggt ggcgtgtgag gcaatacttt tcaggccaaa tgcctcgctc
    21601 atacgggaga ttttttcgtt ctgcgcccga ccgcgtaagc catacacacc agagaaaaag
    21661 cgtaaattct gttcgaccgt caggttgccg tagagcgaaa atttttgcgc catatagccg
    21721 agatgctggc gcgctttacc ggaactctct ttcagatcca tccccagcac cagcgcctgg
    21781 ccggaagtcg gcaccagcaa accgcacatc atcttaaagg tggtcgattt acccgcgccg
    21841 tttggcccca gcaaaccaaa aatctcccca cgtttaacgg caaagttgac gtgatcggtg
    21901 gcggcaaaat caccaaattt cttggtcagt tctttcgctt cgatcaccgt ctcgccgggt
    21961 gtgccttcta ccgtatgcaa tattgcaccc agcggcgatt ctgaggttcc ggcaccgccc
    22021 agcaaatcaa taaacgcatc ttcaaaacgc ggcgtagttt cgttgatgtt gatttccggc
    22081 atcccgtcgg catggcgaat atcgtctggt gtggcctctt ttttgaggat cagacgtacc
    22141 gatttcccct gaatcatgcc atcgctgacc tgcggcagtt tcaaggcgcg ttgcaacagt
    22201 ttgcggttgc cctcgtgtgg actggtcatc agaaagctgc gtccggccat ggtttgtgtc
    22261 agggcttttg gttctccctg atacagcaac tcgccttcgt tcatcagtaa cacgtcacgg
    22321 cactgctcgg cttcgtcgag atacgaggta ctccagagga ttaacatccc ttcgcccgcc
    22381 agctcatgca ccatctgcca cagttcgcgc cgtgagatag ggtcaacgcc gacgccgggt
    22441 tcatcgagca gcaacacttt cggttcgccc accagggtac aggccaggcc gagtttttgt
    22501 ttcatcccac cggagagctt gcccgccagg cgtccggtaa acggcccaag agacgtaaac
    22561 tccagcaggc gagcaaaagt ttgcttacgt gcctcgccgg tgacgctgcg caaatccgcg
    22621 tacagattga ggttctccat caccgtgaga tcttcataca gaccaaattt ctgcggcata
    22681 tagccgagca cggcgtgcag cgcgccgtcg tttttgatcg gatcaaagcc aatcaccgtg
    22741 gcactgccgc tgtcgggttt cagtaatccc gccaacatcc gcatcagtgt ggttttacct
    22801 gcaccgtccg gccccaccaa ccccgtcaca taaccggcgt gaatggtaca atcgagcggc
    22861 gcgacggcgg gcttgtccat gcccggaaag cgtttttcca ggccgttcag cgtgataacg
    22921 gcatcattca tgtcctgcct cgtcaccgaa ttgtaccgtc actggcattc cctggcgtaa
    22981 cgcatcatcg gcgtcggtca ccacaatacg caggcgatag acgaggtcgg tacgcagatc
    23041 cggtgtttcg acggttttcg gggtaaattc agcagtcggc gaaacgaaac caatctgccc
    23101 gtgatacggc ttgtccgggc gaccatcggt ataaagcagc actttgcgcc ccggctgggc
    23161 ctggtcaaga ttccgttcat caacataagc gcgcacccac accggacgcg ttagtgaaac
    23221 ggtaaacacc gtgccacctt cattgagaac cgtgcctggc tctaccgcgc gcgttaacag
    23281 cgtgccatca gacggtgcta tcaacgttga gtcctgtaaa ttcagctccg cctgcgccag
    23341 ttgcgcctgc gcctgttcga ggctggcttt cgcctgagcg atgtcctgtt cacggttacc
    23401 ggaacggtac tgacgcaatt tatcctgtgc tgatttcagc gttgcctgtg cctggtcgcg
    23461 cgaggagcgg gcattttcca ggtcatttgc cgaaatggtg cggcttttcc acagtccttg
    23521 ctggcggtta tagaagttct gcgcatagtc ataggcggct tgcgcctgtt tcaccgctgc
    23581 ggcggcctga gcgatttctt catcgcgata cccggcaagc atcagatcat actgcgcctg
    23641 tgccaccgag acgcccgctt tcgcctgcat cagggcaatc tcatacggct tgtgatccag
    23701 ttcgcccagc acctggcccg ctttgatagc atcaccttcg tccaccgcca gcgattcaac
    23761 gcgcccccca acacggaaac taagattcac cgtacgaata tccacgttgc catacagcgt
    23821 caggccgtta tcctggcggc tttgatacca ccagtagcct ccggcaacca cggcggcaag
    23881 taccactacc gccagtccga tcacgacagg ttttttcatc actacaaact cctttgcgat
    23941 aatccttgca aaatcaggtc gatatgacag gtcaccgtct ggttgatcag ttcggtcttt
    24001 tcttcatcga acgcggtcca gccggtacgt aacagaattg tttctttgcc aagacggaac
    24061 gccagaatct caccaatcaa cgcatgggta tgaaggatca tgcgggtgtc attagcgtcg
    24121 cagccggtcc aggcggcaat caggcgtgtc aggtggctgt gtagcggact aatcacctgt
    24181 tcgtgcacca ggtggtaggc tgccgtggga gagagctgct cacgggagat aaacttgctg
    24241 aggttgacgg tgtcatcctg ggtgagcagc ttaatcatgt tcctgcaggc gcgaaggatc
    24301 agttcacgga tggcagcccg atcaggctgt ggttgggcga acaagcgttc ggcttcctcg
    24361 gcatgcggac ggaactgctc gccaataaaa tcggcaatcc actgggcgca ggcgaggtac
    24421 aaatcttctt tcgaaccgaa gtagtaggtg atggcagcga tattctgccc ggcctgggcg
    24481 gctatctcgc gggtggtggc gttcattcca tattcaccaa actgcgccag tgcggcagca
    24541 atcagctgtt ttttcgcctg ttcacccttg attgtcatgg caggattatt catcgcacag
    24601 tccattctta atcaaatgat tgattaagat tatgactcca tatgggagtt gtccagtatg
    24661 gctaagaatt ttagctacgc cagtcacagg gataatttat gcgctgcgtc acaaaaactg
    24721 ctacactccg ctccctcatg acattgtggt ttttgtcatt ttccttttca gtatctccct
    24781 gaaaactaca ccggtaacgg tcggggcggt tcggagtagt tatgtctttc gattctttgg
    24841 gtttaagccc tgatatcctg cgcgccgttg ccgagcaggg ttaccgtgaa cccaccccta
    24901 ttcagcagca ggcgatccct gctgtgctgg aaggccgcga cctgatggct agcgcccaga
    24961 ccggcaccgg caaaacagcg ggctttacgc tgccgctgtt gcaacacctg atcactcgcc
    25021 agccgcacgc caaagggcgt cgtccggtac gtgcgctcat tcttaccccg acccgtgaac
    25081 tggcggcaca gattggcgaa aacgtccgtg attacagcaa atacctgaac attcgttcgc
    25141 tggtggtgtt tggcggtgtc agcattaacc cacaaatgat gaagttgcgc ggcggcgttg
    25201 atgtgctggt ggcaacgccg ggacgtttgc tggacctgga acatcaaaat gcagtgaagc
    25261 tggatcaggt tgaaatcctc gtcctcgatg aagctgaccg catgctcgac atgggcttta
    25321 tccacgatat ccgtcgcgtg ttaacaaaac tgccagcgaa acgccagaac ctgctgttct
    25381 ctgcgacctt ctctgacgat attaaagccc tggcggaaaa actgttgcac aacccgctgg
    25441 aaatcgaagt ggcacgccgc aataccgcgt ctgatcaggt gacccagcac gttcactttg
    25501 tcgataagaa acgcaaacgc gaattgctgt cgcacatgat tggtaaaggg aactggcagc
    25561 aggtgctggt gtttacccgt accaaacacg gcgctaacca tctggctgag cagctcaata
    25621 aagatggcat ccgtagtgtg gcgatccacg ggaataaatc gcaaggtgcg cgtacccgtg
    25681 cgctggctga ttttaaatcg ggtgacattc gcgtactggt ggcgaccgat atcgctgcgc
    25741 gcggtctgga tatcgaagag ttgccgcacg tcgtcaacta tgaactgcca aacgtaccgg
    25801 aagattatgt ccaccgtatc gggcgtaccg gtcgtgcggc tgctaccggt gaagcgttgt
    25861 cgctggtgtg tgttgatgaa cacaaactgc tgcgtgatat cgaaaaactg ctgaaaaaag
    25921 agatcccgcg cattgcgatt ccgggctatg agccggaccc gtcaatcaaa gccgaaccga
    25981 tccagaacgg tcgccagcaa cgtggcggca tcaggctgat gaacaaacgc aaaactgcct
    26041 gatgcgctac gcttatcagg cctacgtgaa ctctgcaata tattgaattt gcatgctttt
    26101 gtaggccgga taaggcgttt acgccgcatc cggcatttca caacaagcac ttgtcagcaa
    26161 tttgagaaca cgggaaaaaa tttatctgtt ttaccgccca taagccaccg agacgttact
    26221 tctctatagc caattgttcg cgcaactcca ttaaaaggta gcccagtcga ttcttgccct
    26281 taccattacc accgtctccc cagtaagcat cgttttccgt atgctcaacc agttttgcgg
    26341 gcgcggttgc cagcaagagc acacgcagtt ctgcatgctg ttcgaattta gcgcgaagtg
    26401 ctttacgcat cacctgttct ttaaccgact cccagttttt acgaagtggc ttggaacgat
    26461 tacgccccat gcgagcggcg accatggggg aagaaacccg acggatctct tcgcggtatt
    26521 tttcatcaag gaatttttgt gcctgaaaat agtgttcgga ggtgggccag gtattcccgt
    26581 caaccttgat gggcctggcg gcaaagttag aaaaatcacc gtagtcatcg ctggtgctgt
    26641 agaaatttat gatggtgtct tgcatgacgt gttggatcct ttgtgctcga acgggcatta
    26701 aaccgcatta tgttggtggt tattgcgagc cgctttccag aaacagaaaa accattaccc
    26761 ctgaaaaccg aaaaatgcca caatattggc tgtttataca gtatttcagg ttttctcatg
    26821 gcattaaccg ccgcgcttaa agcgcaaatt gccgcctggt ataaggcgct tcaggaacag
    26881 atccccgact ttattccccg tgcgccgcag cggcagatga ttgcggacgt cgccaaaacg
    26941 ctggccggag aagaagggcg gcatctggcg attgaagccc ccaccggcgt tgggaaaacg
    27001 ctctcctatt tgatccccgg catcgccatt gcccgcgaag agcaaaaaac gctggtggtg
    27061 agtaccgcca acgtggcgtt gcaggatcag atttacagca aagatttacc gctgctgaaa
    27121 aagatcattc ccgatcttaa attcactgcc gctttcgggc gtgggcgcta cgtttgtcca
    27181 cgtaatctga cggcgctcgc cagcactgaa cccacgcaac aggatctgct ggcgtttctt
    27241 gacgacgaac tgacgccaaa taatcaggaa gagcaaaaac gttgtgcgaa gctgaagggc
    27301 gatctcgaca cttataaatg ggatggtctg cgcgatcata cggatatcgc tatagatgat
    27361 gatctctggc gtcgtttgag taccgacaaa gccagctgcc tcaaccgtaa ctgttactac
    27421 tatcgtgaat gcccgttttt tgttactcgt cgggagattc aggaagcgga agtggtggtg
    27481 gcaaaccatg cgctggtgat ggcggcgatg gaaagcgaag ccgtactgcc tgacccgaaa
    27541 aatctgctgc tggtgctgga cgaaggtcat cacctgccag atgtggcccg ggatgcgctg
    27601 gagatgagcg ctgaaatcac cgtgccctgg tatcgactac agctggactt gttcacgaaa
    27661 ctggtcgcta cctgcatgga gcagtttcgc ccgaagacca tcccaccgct ggcgatccct
    27721 gaacgtttga atgcgcattg tgaagagttg tatgagctta tcgcctcgtt aaacaacatt
    27781 ctcaatctct acatgcctgc cgggcaggag gcagagcacc gttttgcgat gggcgaactg
    27841 cctgatgaat tgctggagat ctgccagcgg ctggcaaaac tcaccgagat gctgcgtggt
    27901 ctggcggagt tatttcttaa cgatttaagt gagaaaaccg gcagccacga tattgtgcgt
    27961 ctgcatcggt tgattttaca gatgaaccgc gcgctgggga tgtttgaggt gcaaagcaaa
    28021 ctctggcggc tggcgtctct ggcgcaatct tccggtgcgc cggtgaccaa atgggcgacg
    28081 cgggaagagc gcgaagggca gctacacctc tggtttcact gcgtgggaat acgtgtcagc
    28141 gatcagctgg aaaggctgct gtggcgcagt attccgcaca ttattgtcac ctccgcaacc
    28201 ttgcgttcgc tgaatagttt ttcgcgtttg caggagatga gtggtctgaa agagaaagcg
    28261 ggcgaccgtt ttgtggcgct ggattccccc tttaaccact gcgaacaggg caaaattgtt
    28321 attccccgga tgcgctttga gccttccatc gacaacgaag agcaacacat tgccgaaatg
    28381 gcggcctttt tccgtgaaca ggtggagagc aaaaaatatc tcggtatgtt ggtactgttt
    28441 gccagcggac gggcgatgca gcgctttctc gactatgtga cggatttacg cctgatgttg
    28501 ctggtgcagg gcgatcagcc gcgttatcgc ttagttgaac tgcaccgcaa acgcgttgcc
    28561 aacggtgagc gtagcgtgct ggtgggctta cagtcatttg ccgaagggct tgatttgaaa
    28621 ggtgatctgc tcagccaggt gcatatccac aaaatcgctt ttccgcccat cgacagcccg
    28681 gtggtgatca ccgaagggga atggctgaaa agcctcaacc gctatccgtt tgaggtgcaa
    28741 agcctgccga gcgcctcgtt taacctgatt cagcaggttg ggcgactgat tcgaagccac
    28801 ggttgctggg gcgaagtggt tatctacgac aaacgcttgc tgacaaaaaa ttatggcaag
    28861 cgactactgg atgcattacc ggtatttccg atagagcaac cggaagtccc tgaaggtata
    28921 gttaaaaaga aagaaaaaac gaaatcccca cgccgtcggc ggcgttaatg atgtgagtca
    28981 ggtaaggagt cgtaaatgga ctatcgcaaa atcattaaag agatcgggcg cgggaaaaac
    29041 cacgcgcgcg atttagaccg ggatactgcg cgcggtctgt atgctcatat gctcaatggt
    29101 gaagtccctg accttgagtt gggcggcgta ttgattgcgc tgcgtatcaa aggggaaggg
    29161 gaagcagaga tgctcggctt ttacgaagcc atgcaaaatc acaccatcaa gctgacgccg
    29221 cctgcaggca agccgatgcc gattgtcatc cccagttaca acggcgcgcg taaacaggct
    29281 aacctgacgc cattattggc gattctgctg cataaactcg gttttcctgt ggtggttcac
    29341 ggagttagcg aagatccaac tcgcgtgctg acagaaacca ttttcgaact gatgggtatt
    29401 acaccaacgt tgcacggcgg gcaggcgcag gcgaagctcg acgaacatca accggtgttt
    29461 atgcctgtcg gcgcgttttg cccgccgctg gaaaaacaac tggcgatgcg ctggcggatg
    29521 ggcgtgcgta acagtgcaca taccctggcg aaactggcga cgccatttgc cgaaggtgag
    29581 gcactgtgtc tttccagcgt ttcgcatccg gaatacattg gacgcgtcgc gaagttcttt
    29641 agcgatatcg gcgggtgggc gttactaatg catggtactg aaggcgaagt gtatgctaat
    29701 ccgcagcgct gcccgcagat caatctcatt gaccgtgaag ggatgcgggt gctgtatgaa
    29761 aaacaggacc ctgctggtag cgagttactg ccacaagcaa aagatccgga aaccacggcg
    29821 cagtggactg agcgttgcct ggctggcagc gaagcgattc ccgaatcgct gaaaatccag
    29881 atggcttgct gcctggtggc tacgggtgaa gcggcaacta tcagcgatgg cctggcgcgc
    29941 gttaatcagg cattttaatt tttatctcag ccggatgagc cacgtcatcc ggcttttacc
    30001 ccaccgcaat atgaaattcc tgcatcttta ttgaccttcc cgcgcccggc gtgcagcata
    30061 aaaaaacaac aaacacataa cataaacagg agttaaccat ggaaagtggt catcgctttg
    30121 atgctcagac gctgcacagt tttattcagg ctgtatttcg tcagatgggt agcgaggaac
    30181 aagaagcgaa attagttgcc gatcatttaa tcgcggcaaa cctggcaggg catgattctc
    30241 atggtattgg catgatccca agctatgtgc gttcctggag tcaggggcat ctgcaaatta
    30301 accatcatgc caaaatcgtt aaagaggcgg gggcggcagt cacgctcgat ggcgatcgcg
    30361 cattcggtca ggtcgcggca cacgaagcga tggcgctggg gattgagaaa gcgcatcagc
    30421 acggtattgc tgccgtggcg ctacataact cgcatcatat cggccgtatc ggttactggg
    30481 cggagcagtg ttcagcggcg gggtttgtct ctatccactt tgttagcgtg gtcggtattc
    30541 caatggtcgc gccgttccac ggtcgcgaca gccgctttgg caccaatccc ttctgtgtgg
    30601 ttttccctcg taaagataat ttcccgctgt tgcttgatta cgccaccagc gccattgcat
    30661 ttggcaaaac ccgcgtcgcc tggcataaag gcgtccccgt gccgccaggt tgcctgattg
    30721 acgttaacgg cgtgccgacg accaatccgg cggtaatgca ggagtcgccg ttggggtcgc
    30781 tgttgacctt tgccgaacat aaaggctacg cccttgcagc gatgtgtgaa attcttggcg
    30841 gggcgctttc tggcggtaaa acgacgcatc aggaaacgtt gcaaaccagc cccgatgcca
    30901 ttcttaactg catgaccact atcatcatca acccggaact gttcggcgcg ccggattgta
    30961 acgcgcagac cgaagccttt gccgagtggg tgaaagcctc gccgcatgat gacgataagc
    31021 cgattttgct accgggcgag tgggaagtga acacgcgtcg cgaacggcag gagcagggga
    31081 ttccattgga tgcgggaagc tggcaggcca tttgtgatgc ggcgcggcag attggtatgt
    31141 cggaagagac gttgcaggct ttctgtcagc agttagccag ctaaaaaaaa gcccgtccag
    31201 tggcggacgg gcaaacaagg gtaacatagg atcaatgagg gttagagcat atgcgtctgt
    31261 cggcaaacag acagggaaat acttgtgctg gacgtagcgt aaacgcctga tccgtccaac
    31321 ggttcggatt ttgtaggcct gataagacgc ggtagcgtcg catcaggcat ctgttgccgg
    31381 atgcggcata aacgccttat ccgtcctacg aattacttat aaattaccgc agtaccgctt
    31441 aatttattgt tgttggtggc ggaagtgatg ctgtatccac tggcaccggc tgccgccgct
    31501 ttctcagcca gtttcgcttc cagggcatcg agggtggatg cgccatcggc agaaaccacg
    31561 ccgattttat tcatgttctg tgcctgggat gccgttaccg gttccgccgc gaacacgcca
    31621 aatgacagag ttgaaagagc catagcagca acaacagtat tgatagtttt cataattaat
    31681 ctctcgcagg tgatttttgt ataagtgacg ttgtttcgtc gatgtgatga gtatcacgtt
    31741 tttttacgag agataaaatc gaagagaatt gaccgccttg ttcaaataaa ttgattgata
    31801 tctaacatac tgataaacca tttatttgtt gtacttatgg acttgtttta tgccgcgtgg
    31861 cagtcacagc agcgttaaag gcacggaatg ccacgcaaaa tacaaattat actcgctatg
    31921 catctcgcca gttgtagtta acgtaactgt ctgtctttcg aacctctgcg attatatcct
    31981 gtatacgctg gtttttgtaa atctttctcc tgctgacaat gaatacataa gcgcacgcca
    32041 ggaatggctt cccgacgggc ttgcgggatg ggggcaccgc actcttcaca ttcatacagg
    32101 ctttcgccac gcggaatttc accccgagcg cgggcaatcg catcttcaat tgtactgttg
    32161 atctgttcgt tgacggcgtc atcgttagcc caagcggatg ccatatcgtc ctccccatat
    32221 caatgcttgt acagttaagt gtagctaata tagggacgaa cctgggcagt tcaagcgtca
    32281 gatctccgac cattcccgca gcagattatg ataaagattg agcagcgaca ggatctcttc
    32341 attttcacca tagcggcttt tcagcgactg aatatttttg tccagttcaa acagcatggc
    32401 gcgcttttta tcatcgcgga tcatcgactg gatccacata aatgatgcca ctcgtacgcc
    32461 gcgggttacg ggtgtcacgc aatgcaggct gctggaggga tacaacacca gatcacctgc
    32521 cgggagtttt acccgatgtt gtccaaaggt gtcattaacg accagttcgc cgccgtcgta
    32581 gctttgtgga tcgcttaaaa acagcgtggc agaaaggtca gtgcgcatcc agccgttttg
    32641 tggatggctg cgtactgcgc catccacatg gaaaccatag gtttcattgt tctgatagcg
    32701 attaaacagc ggcgtggaaa gggtacgcgg caaggcagcg gcaaagaaca atgcatgttg
    32761 gttaaccgcg ttcagcacct cattttgcag tgcggcgtat aacgcgctgc gggtgtctac
    32821 ttgctgattg ttcttaactt gtgcaccttg tgctccggtg gtgacgcgtc catccaccca
    32881 ttcggcttgt tccagttgtt cgcgaaaatg ggcgacgtcc tgtggcgata acacgccggg
    32941 aatgtggtac atcatacaag ttctccaaaa agtggggcct gcgccccaca tctgaatcag
    33001 aaatgcatat tggctgtgag caagaaggtt cttggctcgc ccgggtgata acggtagccg
    33061 ctcttgttga ttgaggcgac gtaatcggta tcaaacaggt tgtaaacgtt tagctggaag
    33121 tcgagattgc gattaacccg ataccccagt ttggcatcgg cgacccagta accttctgta
    33181 aatgctggcg ttcccaccgc gccgtctgaa cctttatgta tactgccgat atagcgtgcg
    33241 cccgcgccaa cagagatatc atcggttgcc tgatattggc tccataaggt gaaggcgtgc
    33301 tccggggtat acggcagcga tgaggaacca tcctgggcaa catctttgcc gtttttgatg
    33361 gttgcttttt gctgggtata gccgccaatc acctgccacg cgggagtgat attccctgcc
    33421 acggatatct catagccttc gacgcgtttc ttaccgtatt gcgagtaagt cccgtcatca
    33481 ttttgctcaa cttcattttc gatatcagta cggaacagcg cggcggtgag caacaggcgt
    33541 ttatccagaa cctgccattt ggtgccaatc tcgctggtgt tggctttttg cggtttaaaa
    33601 tcggtgcggt tggcactgtt accactgcca gactgcgcaa gggcgaagtt gttgccgccc
    33661 ggaggctgct gggaaacggc atagttaata tagacattgc cgttttccgt caggtggtac
    33721 agcgccccgg ctttccagtt caccagattg cccgacttgg cggtgtcgac ggtggtgact
    33781 ggagaacctt ttgccacacc agctgggcag gtgatggcac cgcgtccgct gccgccgcag
    33841 gcggtggcac tgtcatattc agtatgataa ttatccagac ggatcccgcc gttcagctca
    33901 aaatcacggg tgatttgcag cgtatcgaag gcgtaaattg cgaaggtatc cgtctgacca
    33961 ttggcgtttg cgccgttgcg cgtcaggccg ccgggatgaa ttctgctgtc aggatgataa
    34021 atatttaccg ccggtaacgt caccggatta acgccatagt tagtctgcgt ttcacgggta
    34081 aattccacgc cggtactgac atcatgaccg atagaaccgg tatagaacgt cgaggtcagg
    34141 ttggtctggt tggtgagaat tttattactc acatctttgg tattcgccgt gcgtgactag
    34201 gtccagctat tcacatcgct ggtgggctga gtaatattcg acgccccgcc cataatcgcc
    34261 gtcatcaggt aatcctgctt tacgcgcgac caacgggtag tattgcgaat ggtggtgtta
    34321 tcgttgatgt cgtgctcaaa acgcatggtg gcggtgtcgg tggtcgaatc gtcgtaatcg
    34381 gaatccgtgc cgtaaaagtt atgagtatca acttttccgg aatgattcag ggccgccgct
    34441 cccgcagatg gggcagaata gcccggcaaa ccgatggtcg gaatgccgcc gtctggcgtg
    34501 ttgtgctgag tgacatgcag ataattaaga tacaaacgat ttgctgtacc aaggccaaaa
    34561 gcgacagaag gggcgacgcc gtaacgctca tttttgactt tgtcgcgtcc ggcatcgtgg
    34621 gttttttcgc ccatcacatt caggcgcacc gcggtagtat caccaatgac ctgattgacg
    34681 tccagcgtgc cgcggcggaa ccaggcgctg ccaatactgg cggaggcgtc aatgccggaa
    34741 tcattgcgcg gctgcttgct gatcatattg atcgagcctg tcggtgcgct gcgcccgtag
    34801 tcggtgccgg acggcccttt aatcacttcg acctgctcgg tattgaaggt gtcgcgcgag
    34861 acgctgccga tatcgcgaat accatcaata taaatactgt tagaggtatc ggcaccacgc
    34921 atataaatgg cgtcgccagt ggtggagtta ccgttctcac ccgcaaaaaa cgcacccacg
    34981 c
//
LOCUS       DAJTSI010000025        34903 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000025 DAJTSI010000000
VERSION     DAJTSI010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34903
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.025"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            858..3143
                     /gene="nrdA"
                     /locus_tag="OO327_001119"
     CDS             858..3143
                     /gene="nrdA"
                     /locus_tag="OO327_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR6017743.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3377..4507
                     /gene="nrdB"
                     /locus_tag="OO327_001120"
     CDS             3377..4507
                     /gene="nrdB"
                     /locus_tag="OO327_001120"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR6017744.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4507..4761
                     /gene="yfaE"
                     /locus_tag="OO327_001121"
     CDS             4507..4761
                     /gene="yfaE"
                     /locus_tag="OO327_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR6017745.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4815..5465)
                     /gene="inaA"
                     /locus_tag="OO327_001122"
     CDS             complement(4815..5465)
                     /gene="inaA"
                     /locus_tag="OO327_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCR6017746.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5644..5886
                     /locus_tag="OO327_001123"
                     /pseudo
     CDS             5644..5886
                     /locus_tag="OO327_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5928..7004)
                     /gene="glpQ"
                     /locus_tag="OO327_001124"
     CDS             complement(5928..7004)
                     /gene="glpQ"
                     /locus_tag="OO327_001124"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6017747.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(7009..8367)
                     /gene="glpT"
                     /locus_tag="OO327_001125"
     CDS             complement(7009..8367)
                     /gene="glpT"
                     /locus_tag="OO327_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR6017748.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8640..10268
                     /gene="glpA"
                     /locus_tag="OO327_001126"
     CDS             8640..10268
                     /gene="glpA"
                     /locus_tag="OO327_001126"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR6017749.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            10258..11517
                     /gene="glpB"
                     /locus_tag="OO327_001127"
     CDS             10258..11517
                     /gene="glpB"
                     /locus_tag="OO327_001127"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR6017750.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11514..12704
                     /gene="glpC"
                     /locus_tag="OO327_001128"
     CDS             11514..12704
                     /gene="glpC"
                     /locus_tag="OO327_001128"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR6017751.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12897..13847
                     /locus_tag="OO327_001129"
     CDS             12897..13847
                     /locus_tag="OO327_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR6017752.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13888..14358)
                     /locus_tag="OO327_001130"
     CDS             complement(13888..14358)
                     /locus_tag="OO327_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR6017753.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14413..15615)
                     /locus_tag="OO327_001131"
     CDS             complement(14413..15615)
                     /locus_tag="OO327_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR6017754.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15702..16244)
                     /locus_tag="OO327_001132"
     CDS             complement(15702..16244)
                     /locus_tag="OO327_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6017755.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16523..16948
                     /gene="nudI"
                     /locus_tag="OO327_001133"
     CDS             16523..16948
                     /gene="nudI"
                     /locus_tag="OO327_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR6017756.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16987..17589)
                     /locus_tag="OO327_001134"
     CDS             complement(16987..17589)
                     /locus_tag="OO327_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR6017757.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17897..19036
                     /gene="arnB"
                     /locus_tag="OO327_001135"
     CDS             17897..19036
                     /gene="arnB"
                     /locus_tag="OO327_001135"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR6017758.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            19040..20008
                     /gene="arnC"
                     /locus_tag="OO327_001136"
     CDS             19040..20008
                     /gene="arnC"
                     /locus_tag="OO327_001136"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR6017759.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            20008..21989
                     /gene="arnA"
                     /locus_tag="OO327_001137"
                     /pseudo
     CDS             20008..21989
                     /gene="arnA"
                     /locus_tag="OO327_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            21986..22876
                     /locus_tag="OO327_001138"
     CDS             21986..22876
                     /locus_tag="OO327_001138"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR6017760.1"
                     /translation="MTKVGLRIDVDAFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22876..24528
                     /gene="arnT"
                     /locus_tag="OO327_001139"
     CDS             22876..24528
                     /gene="arnT"
                     /locus_tag="OO327_001139"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR6017761.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24525..24860
                     /gene="arnE"
                     /locus_tag="OO327_001140"
     CDS             24525..24860
                     /gene="arnE"
                     /locus_tag="OO327_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR6017762.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24860..25246
                     /gene="arnF"
                     /locus_tag="OO327_001141"
     CDS             24860..25246
                     /gene="arnF"
                     /locus_tag="OO327_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR6017763.1"
                     /translation="MCLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25240..25512)
                     /gene="pmrD"
                     /locus_tag="OO327_001142"
     CDS             complement(25240..25512)
                     /gene="pmrD"
                     /locus_tag="OO327_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR6017764.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25617..26972)
                     /gene="menE"
                     /locus_tag="OO327_001143"
     CDS             complement(25617..26972)
                     /gene="menE"
                     /locus_tag="OO327_001143"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR6017765.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26969..27931)
                     /gene="menC"
                     /locus_tag="OO327_001144"
     CDS             complement(26969..27931)
                     /gene="menC"
                     /locus_tag="OO327_001144"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR6017766.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(27931..28788)
                     /gene="menB"
                     /locus_tag="OO327_001145"
     CDS             complement(27931..28788)
                     /gene="menB"
                     /locus_tag="OO327_001145"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR6017767.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28803..29561)
                     /gene="menH"
                     /locus_tag="OO327_001146"
     CDS             complement(28803..29561)
                     /gene="menH"
                     /locus_tag="OO327_001146"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR6017768.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29558..31228)
                     /gene="menD"
                     /locus_tag="OO327_001147"
     CDS             complement(29558..31228)
                     /gene="menD"
                     /locus_tag="OO327_001147"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR6017769.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31317..32612)
                     /gene="menF"
                     /locus_tag="OO327_001148"
     CDS             complement(31317..32612)
                     /gene="menF"
                     /locus_tag="OO327_001148"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR6017770.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLLPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32691..32996)
                     /gene="elaB"
                     /locus_tag="OO327_001149"
     CDS             complement(32691..32996)
                     /gene="elaB"
                     /locus_tag="OO327_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR6017771.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(33051..33512)
                     /locus_tag="OO327_001150"
     CDS             complement(33051..33512)
                     /locus_tag="OO327_001150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6017772.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33577..34494
                     /gene="rbn"
                     /locus_tag="OO327_001151"
     CDS             33577..34494
                     /gene="rbn"
                     /locus_tag="OO327_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR6017773.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34685..34873
                     /locus_tag="OO327_001152"
                     /pseudo
     CDS             34685..34873
                     /locus_tag="OO327_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         8537 a         9340 c         8648 g         8378 t
ORIGIN      
        1 cgtaatgcct gaaaggcttt gcctgctggc gtgacaactc gctttgatat catatccctg
       61 gcataaatca atggccgccg cgacaccgtt agcagagaaa agagatgcaa tcattgacgg
      121 gagtagagat aaatactcct tgcgtagaaa gataatccgc atattaatct gaccatcatt
      181 ttatataaga cggcatattt aaacgcatta tacaatatgg gttatctttc ttttgtaaat
      241 tgtccaaccc gatgcaatgg ctattttgtt attatttgat gaataatatc agtgcgtcat
      301 aattcaagtt aataacctta agggatacca gttatattta aactaaatta aagtaatgaa
      361 taattttctt ataatataag gtaaattaac aaaatggctt cgcatttaac aataaccgaa
      421 tagaaaacaa ccatttcgac atcaacaatc gcttacattc gcttatatat tgaccacaac
      481 tgatacatca gattatgtga tgactcgtgc ttagatcaat ttttgcaatc attagcaaaa
      541 agattaataa accatctata tcaatttatc caacctattc tgccgttcaa gaaatcgccg
      601 aacagttatt tttaacaaat ttttctcttc ccattgactt tcccggacac cttgtctgac
      661 ctaaggtgcg cgaaagccac tttttccttc ctgagttatc cacaaagtta tgcacttgca
      721 agagggtcat tttcacacta tcttgcagtg aatcccaaac atacccccta tatatagtgt
      781 tctaagcagc ttcccttact acaggtagtc tgcatgaaac tattgcggaa agaattccaa
      841 aaacaggtac gacatacatg aatcagaatc tgctggtgac aaagcgcgac ggtagcacag
      901 agcgcatcaa tctcgacaaa atccatcgcg ttctggattg ggcggcagaa ggactgcata
      961 acgtttcgat ttcccaggtc gagctgcgct cccacattca gttttatgac ggtatcaaga
     1021 cctctgacat ccacgaaact attatcaagg ctgccgcaga cctgatctcc cgtgatgcgc
     1081 cggattatca gtatctcgcc gcgcgcctgg cgatcttcca cctgcgtaaa aaagcctacg
     1141 gccagtttga gccgcctgcg ctgtacgacc acgtggtgaa aatggtcgag atgggcaaat
     1201 acgataatca tctgctggaa gactacacgg aagaagagtt caagcagatg gacacgttta
     1261 tcgatcacga ccgtgatatg accttctctt atgctgccgt taagcagctg gaaggcaaat
     1321 atctagtaca gaaccgcgtg accggcgaaa tctacgaaag cgcccagttc ctttatattc
     1381 ttgttgccgc gtgcctgttc tcgaactacc cgcgtgaaac gcgcctgcaa tacgtgaagc
     1441 gtttttatga cgcggtttcc acatttaaaa tttcgctgcc gacgccaatc atgtccggcg
     1501 tgcgtacccc gactcgtcag ttcagctcct gcgtactgat cgagtgcggt gacagcctgg
     1561 attccatcaa cgccacctcc agcgcgattg tgaaatatgt ttcccagcgt gccgggatcg
     1621 gcatcaacgc cggacgtatt cgtgcgctgg gcagcccgat tcgcggtggc gaagcgttcc
     1681 ataccggctg tatcccgttc tacaaacatt tccagacagc ggtgaaatcc tgctctcagg
     1741 gcggtgtgcg cggcggtgcg gcaacgctgt tctacccgat gtggcatctg gaagtggaaa
     1801 gcctgctggt gttgaaaaac aaccgtgggg ttgaaggcaa ccgcgtgcgt catatggact
     1861 acggggtaca aatcaacaaa ctgatgtata cccgcctgct gaaaggtgaa gatatcaccc
     1921 tgttcagccc gtccgacgta ccggggctgt acgacgcgtt cttcgccgat caggaagagt
     1981 ttgaacgcct gtataccaaa tatgagaaag acgacagcat ccgcaagcag cgtgtgaaag
     2041 ccgttgagct gttctcgctg atgatgcagg aacgtgcgtc taccggtcgt atctatattc
     2101 agaacgttga ccactgtaat acccatagtc cgtttgatcc gaccatcgcg ccagttcgcc
     2161 agtctaacct gtgcctagaa attgccctgc cgaccaaacc gctgaacgac gtcaacgacg
     2221 agaacggtga aatcgcgctg tgtacgctgt ctgctttcaa cctgggcgca attaatagcc
     2281 tggatgaact ggaagagctg gcgattctgg cggttcgtgc acttgacgcg ctactggatt
     2341 atcaggatta cccgatcccg gccgccaaac gtggagcgat ggggcgtcgt acgctgggta
     2401 ttggtgtgat taacttcgct tactatctgg cgaagcacgg taaacgctac tccgacggca
     2461 gcgccaacaa cctgacgcat aaaaccttcg aagcgattca gtattacctg ctgaaagcgt
     2521 ctaatgagct ggctaaagag caaggcgcgt gcccgtggtt taacgaaacc acttacgcga
     2581 aagggatcct gccgatcgat acctataaga aagatctgga tgccatcgct aatgagccgc
     2641 tgcattacga ctgggaagca ttgcgtgaat caatcaaaac gcacggcctg cgtaactcca
     2701 cgctttctgc cctgatgccg tccgagacct cttcgcagat ctctaacgcc actaacggta
     2761 ttgaaccgcc gcgcggttac gtcagcatca aagcgtcgaa agacggtatt ttgcgccagg
     2821 tggtgccgga ctacgagcac ctgcacgacg cctatgagct gctgtgggaa atgccgggta
     2881 acgatggcta cctgcaactg gtaggtatca tgcagaaatt tatcgatcag tcgatctctg
     2941 ctaacaccaa ctacgatccg tcacgtttcc cgtcaggaaa agtgccgatg cagcagttgc
     3001 tgaaagacct gctcaccgcc tacaaattcg gggtcaaaac gctgtattat cagaacaccc
     3061 gtgacggcgc tgaagacgcg caggacgatc tggtaccgtc aatccaggac gatggctgcg
     3121 aaagcggcgc atgtaagatc tgatattgag atgccggatg cggcgtaaac gccttatccg
     3181 gcctacggct cggtttgtag gcctgataag acgcgccagc gtcgcatcag gctccgggtg
     3241 ccggatgcag cgtgaacgcc ttatccggcc tacggctcgg atttgtaggc ctgataagac
     3301 gcgccagcgt cgcatcaggc acaggatgcg gcgtaaaatg ccttatccgg cattaaactc
     3361 ccaacaggac acactcatgg catataccac cttttcacag acgaaaaatg atcagctcaa
     3421 agaaccgatg ttctttggtc agccggtcaa cgtggctcgc tacgatcagc aaaaatatga
     3481 catcttcgaa aagctgatcg aaaagcagct ctctttcttc tggcgtccgg aagaagttga
     3541 cgtctcccgc gaccgtatag attaccaggc gctgccggag cacgaaaaac acatctttat
     3601 cagcaacctg aaatatcaga cgctgctgga ttccattcag ggtcgtagcc cgaacgtggc
     3661 gctattgccg cttatttcta ttccggaact ggaaacctgg gtcgaaacct gggcgttctc
     3721 agaaacgatt cattcccgtt cctatactca tatcattcgt aatatcgtta acgatccgtc
     3781 tgttgtgttt gacgatatcg tcaccaacga gcagatccag aaacgtgcgg aagggatctc
     3841 cagctattac gatgaactga tcgaaatgac cagctactgg caccttctgg gcgaaggcac
     3901 ccacaccgtt aacggtaaaa ccgtaaccgt tagcctgcgc gagctgaaga aaaaactgta
     3961 tctctgcctg atgagcgtta acgcgctgga agcgatccgc ttctacgtca gctttgcttg
     4021 ttcctttgcc tttgcagaac gcgaactgat ggaaggtaac gccaagatta ttcgcctgat
     4081 tgcccgcgat gaagccctgc acctgaccgg tactcagcat atgctgaatc tgctgcgcag
     4141 cggcgcggat gatccggaga tggcggaaat tgccgaagaa tgtaagcagg agtgctatga
     4201 cctgtttgtc caggcggcac agcaagagaa agactgggcg gattatctgt tccgtgacgg
     4261 ttcgatgatt ggtctgaata aagatattct ctgccagtac gttgaataca tcaccaatat
     4321 ccgcatgcag gcagtcggtt tggatctgcc gttccagacg cgctccaacc cgatcccgtg
     4381 gatcaacacc tggctggtgt ctgataacgt gcaggttgct ccgcaggaag tggaagtcag
     4441 ttcttatctg gtcgggcaga ttgacgcgga agtggacacc gacgatttga gtaacttcca
     4501 gctctgatgg cccgcgttac cctgcgcatc actggcacac aactgctgtg ccaggatgaa
     4561 cacccttccc ttctggcggc gctggaatcc cacaatgtgg cggttgagta ccagtgccgc
     4621 gaaggttact gcggctcctg tcgcacccga ctggtcgcag gtcaggtgga ctggattgcc
     4681 gaaccgttag cctttattca gccgggggaa attttgccct gttgttgccg ggcaaaaggc
     4741 gatattgaaa tcgagttgtg atcgggtact agcgccagat acgacgctta cgcgtattat
     4801 ctggcctaca acgattacat ggcgtagtaa tacgctttca cctgctccca gtccgcttta
     4861 gggatcggct ccagatattt ttccagctgg cggaagtcat gattaatcgc tttatcgcga
     4921 cgcaagcggc gacgactttt ctccagatca agaaaaccag cttctgcctt accttctgtt
     4981 ttcacataga tatggcgaac atagcaacag ccatgctgac gattaacgct atgcattttc
     5041 ttaaacgcca gtgccactgc tttcaacatg gcttgccgta cttcgtcaga ataaggcgat
     5101 actgcatgcc gggcatacca gtcagcaatg ctgatgaacc ccgccatatc ttcagtcacc
     5161 agcaacgcgc gccattcacc ctcaattttc accgcttcac caaaaacgat cttcggcacg
     5221 atgacaccgg cccgttccag ttctttaatt accgcaacct cacggacaat cgttggtcgg
     5281 ccgaacggat aacgtactga atgaaacaga tgatgcgtca tgcgctttac atacagcttt
     5341 ttgccgttgc gctcgacgca ttgcaccccg ctcataccat tacggcgata gttaggctct
     5401 tcaacccagt cgccctctgt tgcccaccag tgattaaatt cgtcgtactt tgctgaaact
     5461 gccataccct atcgcctgtc atttttatta acgacaatga ctataggtgg ttacctgagg
     5521 aaaatcttaa tgaaacgtgt cgtattaatg aatttctttt gccgctacag gtgtgacacc
     5581 gcttttgccc ttcatttgca gcgcatagat tccaccagcc actaccacta atagtgcgcc
     5641 cagcagataa aatgtgaatt taatcagtcg gatacagcga tgaattttat tcgacaggga
     5701 ttaggcatcg ccttacaacc agagttaacg ctgaaaagca ttgcaggtga attgtgttcc
     5761 gttcctcacg aaccaacctt ctatcgacag atttcgttgc tgactaaaga aaagccggta
     5821 gaaggcagtc cactgttttt actacaaatg tgcatggaac aattagtggc gattggaaaa
     5881 atttgatatg agacaggatg gcgcatgaac gccatcctgc agtaacatta ctctttatta
     5941 agaaatttta ctgccttatc agggaaatca gtaaacagcc catttacacc cgctttgtta
     6001 tacagagcat catataactg attcacatca ggagtgtatt caggcagttt atctgaccgc
     6061 acggtataag gatgcactac cagtttattc tgctgggcat cttgcaccat gccagtgagt
     6121 ttgatattac ccggctgcga tgtctcctca atcaacatat ggtaatccgg accaatacca
     6181 tctgcatatt ccgccacctg tttcatggct cccggcttaa acatccagtc gtagttgtaa
     6241 ttaacccagc ttccatccgg ctgtttctgc tgcgtttcat tccagtcggt ataggcaatc
     6301 agctgtacca gattgagctc catgcccatt ttgggttcca gctcattctt aatacgcttc
     6361 agctcatcag catcaaaaca ttgcaaataa actttatcgt ctttaccggt gtaaccatat
     6421 ttcttcagca cttccagcgt ttttgccgca atatccttcc cttcctgatg atggaaccac
     6481 ggcgctttga tttctggata gataccgata tttttcccgg tagagtgatt taacccctga
     6541 acaaattcaa tctcttcttc aaaggtgtgc acccggaagt cggacttacc cattgggaaa
     6601 cgccccggat aagtctgtac ttttttaccg ttttcaatat cgaaaccttc ggtaaatttc
     6661 agcgacttaa tttcatccag cgtgaaatct atcgcgtagt aacgaccgtc tttgcgcgcc
     6721 cgatccggga aacgatcggc aacatcagta acacgatcga gataatggtc atgcagaaca
     6781 accagatggt cgtctttggt catcaccaaa tcctgttcca gataatccgc tccctgcgca
     6841 tacgccatcg cttttgctgg cagcgtatgc tccggcaaat atccactggc accgcgatgg
     6901 gcgatgacta ttttttcgtt gctgtccgcc gccattgcac tgcttcccat gactatagtg
     6961 ctcatcatga tcgccatgct aaggtttttc agcttcaatt tcatgccatt agcctccgtt
     7021 gcgtttttgc agtaattgtt catggcgacg tttttcgcca atcatcacaa caatcaacaa
     7081 gataaccgcc agaatgctgc cgccaatcat taccataaag ccgccatccc agccgaagaa
     7141 gtccacggtg tagccaacaa tcgcgctcgc cgccaccgaa ccgcccaggt aaccaaacag
     7201 cccggtaaag cccgctgccg tacctgccgc ttttttcggt gccagttcca gcgcatgcag
     7261 accgatcagc atcacaggac cgtagatcag gaagccgata acaatcatac aaatcatatc
     7321 gacggttggg ttacctgccg ggttcatcca gtaaacgata gtcgcgatgg tcaccagtgt
     7381 cataaagaag acgccggttg ccccacggtt accacggaag actttgtccg acatccagcc
     7441 gcacagcagg gtgcccggaa tacccgcata ctcatagaag aagtaggccc aggaggattt
     7501 atccagcgcg aaatgcttaa cctctttcag ataagtcggt gaccagtcga ggatgccgta
     7561 acgcagcaga taaacgaaca cgttggcgat ggcgatgtac cacagcagtt tgttcggcag
     7621 cacgtactgc atgaagattt gcttcgcggt cagctcctgt tccgcttttt cgttatagtc
     7681 gtccggataa tcatttttgt actcttcgat cggcggcaag ccacaggatt gcggggtatc
     7741 gcgcatcatc gcaaaggcga ataatgccac cagaatggcg cagaaagcag gcatatagag
     7801 cgccgcatgc cagtcattga accaggccat ccccagcagg aacagcagcg gcggaatacc
     7861 accaccgacg ttgtgcgcac agttccacac tgacacaatg ccgccacgtt ctttctgcga
     7921 ccaccagtgc accatagtac gaccacacgg cggccacccc atcccctgga accaaccgca
     7981 gaggaacaac agtacaaaca tcaccgcaat gctcgacgtc gcccatggca caaagcccat
     8041 aaacaacatc actgccgccg ccagaatcaa acctgcgggc aggaaaacgc gcggattcga
     8101 gcgatccgat accgaaccca tgatgaattt cgaaaatcca taagcaatcg agatccccga
     8161 aagggcaaaa cctaaatcac cgcgtgagaa tccctgctca accagataag gcatagcaag
     8221 cgcaaagttc ttacgaacca aatagtaagc cgcatagcca aagaatatcc ccaggaaaat
     8281 ttgccagcgc aatcgacgat aagtcggatc gatctccgcg gcaggtaagc gcgctttgtg
     8341 tggcgctggt ttaaaaatac tcaacattga tagcctccgt ggcccgtggt cttatttatg
     8401 attaacagcc tgattcagtg agagaacctg ccgtttcttg agttgccgcg atgttaagaa
     8461 aacattcata aattaaatgt gaattgccgc acacattatt aaataagatt tacaaaatgt
     8521 tcaaaatgac gcatgaaatc acgttttact ttcgaatcat gagcgaatat gcgcgaaatc
     8581 aaacaattca tgtttttact atggctaaat ggtaaaaaac gaacttcaga gggataacaa
     8641 tgaaaactcg cgactcgcaa tcaagtgacg tgattatcat tggcggcggc gcaacgggag
     8701 ccgggattgc ccgcgactgt gccctgcgcg ggctgcgcgt gattttggtt gagcgccacg
     8761 acatcgcaac cggtgccacc gggcgtaacc acggcctgct gcacagcggt gcgcgctatg
     8821 cagtaaccga tgcggaatcg gcccgcgaat gcattagtga aaaccagatc ctgaaacgca
     8881 ttgcacgtca ctgcgttgaa ccaaccaacg gcctgtttat caccctgccg gaagatgacc
     8941 tctcattcca ggccactttt attcgcgcct gcgaagaagc agggatcagc gcagaagcta
     9001 tagacccgca gcaagcgcgc attatcgaac ctgccgttaa cccggcactg attggcgcgg
     9061 tgaaagttcc ggatggcacc gttgatccat ttcgtctgac cgcagcaaac atgctggatg
     9121 ccaaagaaca cggtgccgtt atccttaccg ctcatgaagt cacggggctg attcgtgaag
     9181 gcgcgacggt gtgcggtgtt cgtgtacgta accatctcac cggcgaaact caggcccttc
     9241 atgcacctgt cgtggttaat gccgctggga tctgggggca acacattgcc gaatatgccg
     9301 atctgcgcat tcgcatgttc ccggcgaaag gatcgctgct gatcatggat caccgcatta
     9361 accagcatgt gatcaaccgc tgccgtaaac cttccgacgc cgatattctg gtgcctggcg
     9421 ataccatttc gctgattggt accacctctt tacgtattga ttacaacgag attgacgata
     9481 atcgagtgac ggcagaagag gttgatattc tgctgcgtga aggggaaaaa ctggcccccg
     9541 tgatggcgaa aacgcgcatt ttgcgggcct attctggcgt gcgcccgctg gttgccagcg
     9601 atgacgaccc gagcggacgt aacgtcagcc gtggcatcgt gctgctcgac catgctgaac
     9661 gcgatggtct ggacggattt atcaccatca ccggtggcaa actgatgacc tatcggctga
     9721 tggctgaatg ggctaccgac gcggtatgcc gcaaactggg caacacgcgc ccctgtacga
     9781 ctgccgatct ggcactgcct ggttcacaag aacccgctga agttaccttg cgtaaagtca
     9841 tctccctgcc tgccccgctg cgcggttctg cggtttatcg tcatggcgat cgcacgcctg
     9901 cctggctgag cgaaggccgt ctacaccgta gcctggtatg tgagtgcgaa gcggtaactg
     9961 cgggtgaagt gcagtacgcg gtagaaaatt taaacgttaa tagcctgctg gatttacgcc
    10021 gtcgtacccg tgtggggatg ggcacctgcc agggcgaact ctgcgcctgc cgcgctgccg
    10081 gactgctgca acggtttaac gtcacgacgt ccgcgcaatc tatcgagcaa ctttccacct
    10141 tccttaacga acgctggaaa ggcgtgcaac ccatcgcctg gggagacgca ctgcgcgaaa
    10201 gcgaatttac ccgctgggtt tatcagggac tgtgtggtct ggagaaggag cagaaagatg
    10261 cgctttgata ctgtcattat gggcggcggc ctcgccggat tactctgtgg cctgcaactg
    10321 caaaaacacg gcctgcgctg tgccattgtc acgcgtggtc aaagcgcgtt gcatttctca
    10381 tccggttcgc tggatttgct gagccatctg ccagatggtc aacccgtggc agacattcac
    10441 agtggactgg aatctttgcg tcagcaggca cctgcacatc cttactctct tctcgggcca
    10501 caacgcgtgc tcgatctcgc ttgccaggcg caggcattaa tcgctgaaag cggtgcgcaa
    10561 ttgcagggca gcgtagaact ggctcaccag cggattacgc cgctcggcac tttgcgtgca
    10621 acctggttaa gttcgccaga agttcccgtc tggccgctac ccgcgaagaa gatatgtgta
    10681 gtggggataa gcgggctgat ggatttccag gcgcatcttg cggcggcttc gttgcgcgaa
    10741 ctggatcttt cggttgaaac cgcagaaata gagctgccgg aactggatgt cctgcgcaat
    10801 aacgccaccg aatttcgcgc ggtgaatatc gcccgtttcc ttgataatga agaaaactgg
    10861 ccgctgttac ttgatgcgct tattcctgtc gccaatacct gcgaaatgat cctgatgccc
    10921 gcctgcttcg gcctggccga tgacaaactg tggcgttggt tgaatgaaaa actaccttgt
    10981 tcactgatgc ttttgccaac gctgccgcct tccgtgctgg gcattcgtct gcaaaaccag
    11041 ttacagcgcc agtttgtgcg ccaaggtgga gtgtggatgc cgggcgatga agtgaaaaaa
    11101 gtgacctgta aaaatggcgt agtgaatgaa atctggaccc gcaatcacgc cgatattccg
    11161 ctacgtccac gtttcgcggt tctcgccagc ggcagtttct ttagtggcgg actagtagcg
    11221 gaacgtaacg gcattcgcga gccgattctc ggccttgatg tgctacaaac cgccacgcgg
    11281 ggtgaatggt ataagggcga tttttttgcg ccgcaaccgt ggcagcagtt cggtgtaacc
    11341 actgatgaga cgctacgccc gtcgcaggca gggcaaacca ttgagaacct gtttgccatc
    11401 ggttcggtgc tgggcggatt tgatcccatc gcccagggat gcggcggcgg tgtttgtgcc
    11461 gtcagtgctt tacatgccgc tcaacagatt gcccaacgcg caggaggcca acaatgaacg
    11521 acaccagctt cgaaaactgc attaagtgca ccgtctgcac caccgcctgc ccggtgagcc
    11581 gggtgaatcc cggttatcca gggccaaaac aagccgggcc ggatggcgag cgtctgcgtt
    11641 tgaaagatgg cgcactgtat gacgaggcgc tgaaatattg catcaactgc aaacgttgtg
    11701 aagtcgcctg cccgtccgat gtgaagattg gcgatattat ccagcgcgcg cgggcgaaat
    11761 atgacaccac gcgcccgtcg ctgcgtaatt ttgtgttgag tcataccgac ctgatgggta
    11821 gcgtttccac gccgttcgca ccaatcgtca acaccgctac ctcgctgaaa ccggtgcggc
    11881 agctgcttga tgcggcgtta aaaatcgatc atcgtcgcac gctaccgaaa tactccttcg
    11941 gcacgttccg tcgctggtat cgcagcatag cggctcagca agcacaatat aaagaccagg
    12001 tcgctttctt tcacggctgc ttcgttaact acaaccatcc gcagttaggt aaagatttaa
    12061 ttaaagtgct caacgcaatg ggtaccggtg tacaactgct cagcaaagaa aaatgctgcg
    12121 gcgtaccgct aatcgccaac ggctttaccg ataaagcacg caaacaggca attacgaatg
    12181 tagagtcgat ccgcgaagct gtgggagtaa aaggcattcc ggtgattgcc acctcctcaa
    12241 cctgtacatt tgccctgcgc gacgaatacc cggaagtgct gaatgtcgac aacaaaggct
    12301 tgcgcgatca tatcgaactg gcaacccgct ggctgtggcg caagctggac gaaggcaaaa
    12361 cgttaccgct gaaaccgctg ccgctgaaag tggtttatca cactccgtgc catatggaaa
    12421 aaatgggctg gacgctctac accctggagc tgttgcgtaa catcccgggg cttgagttaa
    12481 cggtgctgga ttcccagtgc tgcggtattg cgggtactta cggtttcaaa aaagagaact
    12541 accccacctc acaagccatc ggcgcaccac tgttccgcca gatagaagaa agcggcgcag
    12601 atctggtggt caccgactgc gaaacctgta aatggcagat tgagatgtcc acaagtcttc
    12661 gctgcgaaca tccgattacg ctactggccc aggcgctggc ttaaactccc ttctgatgcc
    12721 cggtaagcat gtggttaccg ggcatttttg cgtacacgat tccgtgccca atgtatgcgt
    12781 tgcaacgcag tgaaaattcc tctgaaaacg tctcgcaaag gctgaaactg gcagatgtca
    12841 aaggcctggg ataaccgtaa tgtcgcgtca tcataaatat caggtgacgg acaaccatga
    12901 ccgaatcaac aacctcctcc ccgcatgatg cggtatttaa aacctttata ttcacacccg
    12961 aaaccgcacg ggattttctc gaaatacatt taccagaacc actgcgcaag ctttgcaacc
    13021 tgcaaacctt acgcctggaa cccactagtt ttattgaaaa aagtttacgc gcttactact
    13081 cggatgtttt gtggtccgtg gaaaccagcg acggtgacgg ctatatctac tgcgtgattg
    13141 aacatcaaag ctctgcagaa aagaatatgg cttttcggcc aatgcgctat gccactgccg
    13201 ccatgcagag tcacctagat aaaggctatg acagagttcc gctggtggtg ccattgctgt
    13261 tttatcatgg cgaaacatcg ccctacccgt actcacttaa ctggctggat gagtttgacg
    13321 atccgcaact tgcccggcag ttgtacaccg aagcttttcc gttggtggat atcaccatcg
    13381 tacctgacga tgagatcatg caacatcggc gtatagctct gctggaactg attcaaaagc
    13441 atattcgcga ccacgattta atcggcatgg tcgacaggat caccacgctt ttggttagag
    13501 gcttcactaa tgacagccag ctacaaacac tgtttaatta tctgctgcaa tgcggcgata
    13561 cctcccgttt cacccgtttt attcaggaga ttgccgaacg ttcaccacta caaaaggaga
    13621 gattaatgac tattgctgaa cggctacggc aggaaggaca tcaaattggc tggcaagaag
    13681 gtaaattagt aggtttgcag caaggtaaat tagaaggttt gcaggaaggt atgcatgaac
    13741 aagccattaa aattgctttg cgcatgctgg aacagggcat tgatcgtgac caagtgctcg
    13801 cggccaccca gctaagcgaa gccgatctgg cagcgaataa ccactaatta acacaggccc
    13861 acagccgatc cccatgggcc tttgatatca ataactacct tttatgcgtg gcccattttt
    13921 gcctgattta aacatcgcca gtcgttgatc cagggcatcg ctgtagagca tcgtgtcaac
    13981 gccaacagcg acaaagttcg ctccccacgc caggcattgc tgcgccatat caggagccac
    14041 agccagaaaa ccagccgctt tacccgcagc acggatccgc cgaatgctgg tttcaataat
    14101 tcgctgcact tccgggtgcc cggcgttatc cgggtagccc aacgacgcag aaagatccgc
    14161 tggtccaata aacacgccat caatcccttc gacgtcgagg atctcgtcca ggttatccag
    14221 tgccgtttta ctttccacct gcactaacag acaaagcgaa tcgttgactt gcgccatgta
    14281 attctcaatg cgtccccagc gcgcagcccg tgccacactg gccccaacac cacgctcacc
    14341 gtagggaggg ttggacaaat tttgccggag ggggaatgtg ccggtgcagt aaaccggcac
    14401 agagtggcga aatcagacgg ataaagtcat ggactcaaca acctcaatcc agccatgctc
    14461 actagcgatg tcctggccgt ttaaccaacg gcgtagcata ttcagtgcca tcattgcgca
    14521 cacttcctga cggatagcca ggctgtagcg tgtagtgctg aaacgcacac gcagagcgaa
    14581 agtgccgtct ggcgtggcta gcgcaaagtt gagatgctcg ttctcgaaac ccgaaacagc
    14641 cagtgccagc ccggcaaaat ggttggcccg ccgttctgta atccagtgcg cagtttgcgc
    14701 cagggtttct tcctgtgaag gaatcacttc acacgccagc aatggagcac ctgcgcgaga
    14761 aagttgcaaa gccaataaac caccggtgaa ttgctcgctc aacgtcaggc tgaactggcg
    14821 gttttgcaat tcgcgactga tctgcgcggg cagtccttcg gtgccttcga aaatcacgct
    14881 ctgtccggca acacgtttaa cgtccagcca cagtttttcc atcgcctgtt gctcgcttgc
    14941 cggtccggtg agtttcagtt cgatgatcgg cattgaggag cgatagccca ttgttacgcc
    15001 cggcggcagt tgtagagtgt ccaggctttg tgccagatcg ctttccgaac gaccaaaggt
    15061 agttaaacgc agacaaaccg gcggctgtgg taaagaaaaa cgctcgcgca ggcgcggcag
    15121 gatttcgtgc tcgaccatca ccttaaattc tgacggtacg ccgggagtaa agaacatcag
    15181 gcaacgatta agctgcacgg caaaaccaca ggcggtgcct accgggttat tgataaattc
    15241 agcactggca ggcagctccg cttgtttacg gttgctcggt gccattactc gtccacgttc
    15301 gtggaaatag cgttccatct ctttgagcca ggcttcatgc agcaccaggc cttcaccttt
    15361 tgctgtcgca gcggcgagtg cgcttaaatc atcgctggtc ggtcccagcc cgccgttaac
    15421 gatcagcaca tcggcgtgct gactacgttc gcgaagaatg gtgactaagt catcaaggtt
    15481 atcccccacc gtattgcggc gagataatgg cagcccctga tgaaagaaaa aatcggccag
    15541 ccaggcagcg ttagtgtcaa cgatttgccc gtataacact tcatccccgg tggatagcat
    15601 ttccactttt aacatggtta tctcctgctt ttgttatcga atcactataa cgcaaaggga
    15661 gataaggcgt agtaagaaac tgaatgccgc gccggaaggg atcagaaact ggcgttaacc
    15721 ccaacataca ggccgtcagc cacagcgttg tcgcggttac cgtctttacc cgacagattc
    15781 aggtagcgat aacccgcctc aatactgact ggacgcataa tggtgtaacg cgcgccagca
    15841 ttcgcttcct cataactttg aataccgctg gagagcgaat ccggagagta gtaatactcg
    15901 ccaaacaaac ggaagctgtt gccaattttc cactgcaaac cacctcctac cgctgcggca
    15961 taaccttcat cgccgtaatt cgggttggtg tacacgcctt ttccgccaac ggtcgccatt
    16021 aacggcccga gaggcagatt caaccccagc cccacgcccg cgacgtcacc gtcgtcgtcg
    16081 ttatgtgtcc agttaccgct taaagccagg cccgtcgatt cagtcccaaa accgacacca
    16141 atattggtgt attctttacc cgcctggccg ctgatgctca ttgcattgac cgatgcagaa
    16201 accaacagca ttccggccag acctgctagc gcaatttttt tcattatccc ctttcccaca
    16261 agcaattaat caaaattccc aaagctgcgg gattgtactg cctggcacag aggattcaat
    16321 gagcgaaaaa agggatctag tcacttattg taactaaata cttcttaaat aattaaacgc
    16381 cgaaaaaata aacacacttt accgatacac acaaaaataa taataagtgc agttatcatt
    16441 gcaaaagaga ttactgtcac tttcacatat ttctgagtta ccgtattctc atggtttctt
    16501 tttatttata aggaataccc ctgtgcgaca acggactatt gtatgccctt tgattcaaaa
    16561 tgatggtgct tatttgctgt gcaaaatggc cgacgatcgc ggcgttttcc ccggtcaatg
    16621 ggcgctttcg ggtggcggcg tggagtctgg cgaacgaatt gaagaggcac tacgccgcga
    16681 aattcgcgaa gaactgggag aacagctgct tttgacagaa atcacgccgt ggaccttcag
    16741 cgatgatatt cgcaccaaga catatgcaga tggtcgcaag gaagagattt atatgattta
    16801 cctgattttt gactgcgttt ctgccaaccg agaagtgaaa ataaacgaag agtttcagga
    16861 ctacgcgtgg gtaaaacctg aagatctggt gcattatgat ttaaatgttg ccacccgaaa
    16921 aacgttacgt ttgaaaggtc ttctttaaca atgatgacag ccatcactgg ctgtcagatt
    16981 gaaaatttag tggttaacga attccccatc cagataaact ttgccttttt ccacatgcat
    17041 gactaaacca tccagataat caggtttaaa tatcgcatca cgcttatttt tagcaatata
    17101 tgtcaggcaa tgattatggg tgaaaataac gatattttta tcaggcgctt tgctttgtaa
    17161 gtccttaatt gcactataaa tctcattacc gcactgaaga agtcgtttat ctaccgtcag
    17221 ctttttaccc gcggaaaacc aggtcgccga ctgaatggtc cggacggtat tactggaata
    17281 aagatcgaaa tcagagatgt cagcgctaaa agcgttgccc agttcacggg catcctgggt
    17341 acctttaacc gtaatacctg ttttatctga caagcattga ttggttgaac gatcgcaacg
    17401 ttcagcatgg cgaaacaaaa cgacaaccgg gtgctgctgt gccagtctgg ccaatgtttt
    17461 attgtcgata cgtggcaaac cattgctact ccaggcggca tgagtaccca gtccggcaat
    17521 tgcagcgagc gccagtaaaa tgatgaaata tttttttgac ttcaatgatg agcggcaaaa
    17581 agctaacacg taaactccac ctatagacaa gcgcaaccag acaattaccg tgaaattgag
    17641 cgacatttct ggcgataatt agcagttggt gtaatattaa aaatcctacg atgtcggcaa
    17701 aatgcctcaa atttttgcca aatgcaaagc ctaaataaga aaaaatataa aaatttcaat
    17761 atttacgtct aatattagtt tcttaaggtt aagttaatat tctatcctta aaatttcgct
    17821 ccaaatggca aaatatacac aacactcttt atagcaaata taagtggaca ggtattcaat
    17881 ggcggaagga aaagcaatgt cagaattttt gcctttttcg cgaccagcaa tgggcgtgga
    17941 ggaactcgct gcagttaaag aggttctcga atccggttgg atcacaaccg gtccgaagaa
    18001 tcaggcgctt gagcaagcct tttgccagtt gacgggaaat cagcatgcca tcgcggtcag
    18061 ttcagccacc gccggaatgc atatcacgct aatggcgttg gaaattggca agggcgatga
    18121 agtgattacg ccttccctga cctgggtttc aaccctcaat atgatttcct tgctgggtgc
    18181 aacgccggta atggtggatg tcgaccgcga tacgctgatg gtcacgcctg aagcgatcga
    18241 atcagccatt acgccacgca ctaaagccat cattccggtg cattatgccg gtgcgccagc
    18301 agatattgac gccattcgcg ccattggcga acgttacggc atcgcagtta tcgaagatgc
    18361 tgcccatgcc gtcggtacgt attacaaagg gcgacatatt ggcgcaaaag gtaccgctat
    18421 tttttcattt catgccatta aaaatattac ctgtgctgaa ggtggcctga ttgtaactga
    18481 taatgaaaac cttgcccgcc agctacggat gctgaaattt cacggtctgg gtgtcgatgc
    18541 ctatgacaga caaacctggg gccgtgcacc gcaggctgaa gtcttaacac cgggctataa
    18601 gtacaatctg accgatatta acgccgcgat tgccctgaca cagttagtca aattagagca
    18661 cctcaacacc cgtcggcgcg aaattgccca gcaatatcag caagcactgg cagctctccc
    18721 ctttcagcca ttaagccttc ccgcctggcc gcacgttcac gcctggcatc tgtttattat
    18781 tcgtgtcgat gaacaacgtt gtggtatcag tcgcgatgcg ttgatggaag cgttaaaaga
    18841 aagaggcatt ggtaccgggt tacatttccg cgccgctcac acacaaaaat attatcgcga
    18901 gcgttttccc acgctgtcgt taccgaatac cgaatggaat agcgaacgca tctgttcgtt
    18961 gccgctgttc ccggatatga ctaccgccga tgccgaccgc gtcatcacag cccttcagca
    19021 actcgcagga caataagcca tgtttgaaat ccaccctgtt aagaaagtct cggtggttat
    19081 tcccgtttat aacgagcagg aaagcttacc ggaattaatc aggcgcacca ccacagcctg
    19141 tgaatcgttg gggaaagagt atgagatcct gctgattgat gacggcagta gcgataattc
    19201 cgcgcatatg ctggtcgaag cctcacaagc ggagaacagc catattgtgt ctattttgat
    19261 taaccgcaat tacgggcaac attcagcgat tatggcggga ttcagtcacg ttactggcga
    19321 cttaattatt acccttgatg ccgatctcca gaatccgcca gaagaaatcc cccgcctggt
    19381 ggcaaaagcc gatgaaggtt acgacgtggt agggactgta cgccagaacc gccaggacag
    19441 ctggtttcgt aaaacagctt cgaagatgat taaccggctt attcagcgca ccactggcaa
    19501 agcgatgggt gactacggtt gtatgctgcg cgcctatcgc cgtcatattg tcgatgcgat
    19561 gttgcactgc catgaacgca gcacctttat cccgattctg gcgaatatct tcgcccgccg
    19621 tgcaattgaa attccagtac atcatgccga gcgtgagttt ggtgaatcca aatacagttt
    19681 tatgcgcctg attaatttga tgtacgacct ggtgacctgc cttaccacaa cgccgctacg
    19741 tatgctgagt ctgctcggca gcattattgc gattggaggt tttagcattg cggtgctgct
    19801 ggtgatttta cgcctgacct tcggaccaca atgggcggca gaaggcgtct ttatgctatt
    19861 tgccgtgctg tttactttta ttggcgctca gtttatcggc atgggattac tcggtgaata
    19921 tatcggcagg atctacaccg atgtccgcgc ccgcccccgc tattttgttc agcaagttat
    19981 ccgtccatcc agcaaggaaa atgaataatg aaaaccgtcg tttttgccta ccacgatatg
    20041 ggatgcctcg gtattgaagc tctgctggct gccggttacg aaattagcgc catttttacc
    20101 cataccgata atcccggtga aaaagccttt tatggttcgg tggctcatct ggcggcggaa
    20161 agagacattc cggtttatgc gccggataac gttaatcatc cgctgtgggt ggaacgcatt
    20221 gcccaactgt caccagaggt gattttctct ttttattatc gccatcttat ttgcgacgag
    20281 atttttcagc tcgctcccgc aggtgcattt aatctgcacg gttcgctgtt accaaaatat
    20341 cgtggtcgcg cgccgctgaa ctgggtgctg gtgaacggtg aaacggaaac gggcgttaca
    20401 ttgcaccgaa tggtaaacgt gccgatgctg gggccattgt ggcgcaactg cgcgttgcca
    20461 ttgcgccaga cgatatcgct attacgctgc atcataaatt gtgccatgcc gcgcgccagc
    20521 tactggaaca gacattaccc gccattaaac acggtaatat tctggaaatc gcccagcgcg
    20581 aaaacgaagc cacctgtttt ggtcgcagaa cgccggatga cagtttcctt gaatggcata
    20641 aaccggcatc cgtactgcac aacatggtac gtgccgttgc cgatccgtgg ccgggtgcct
    20701 tcagctatgt tggcaatcag aaattcaccg tctggtcgtc gcgtgttcat cctcatgcca
    20761 gcaaagcaca gccggggagc gtgatttctg ttgcgccact gctgattgcc tgtggcgatg
    20821 gcgcgctgga aatcgtcacc ggacaggcgg gcgacggcat tactatgcag ggctcgcaat
    20881 tagcgcagat gctgggcctg gtgcaaggtt cacgcttgaa tagccagcct gcctgcaccg
    20941 cccgacgccg tacccgggta ctcatcctcg gggtgaatgg ctttattggc aaccatctga
    21001 cagaacgcct gctgcgcgaa gatcattatg aagtttacgg tctggatatt ggcagcgatg
    21061 cgataagccg ttttctgaat catccacatt ttcactttgt cgaaggcgat atcagtattc
    21121 attccgaatg gattgagtat cacgtcaaaa aatgtgatgt cgtcttgccg ctggtggcga
    21181 tagccacgcc gattgaatat acccgcaacc cgctgcgcgt atttgaactc gattttgaag
    21241 agaatctgcg cattatccgc tactgcgtta agtaccgtaa gcgaatcatc ttcccgtcaa
    21301 cttcagaagt ttatgggatg tgtagcgata aatacttcga tgaggaccat tctaatttaa
    21361 tcgtcggccc ggtgaataaa ccacgctgga tttattcggt gtcaaaacaa ttacttgatc
    21421 gggtgatctg ggcctatggc gaaaaagagg gtttacagtt caccctcttc ctcccgttta
    21481 actggatggg accacgactg gataacctta atgcagcgcg aattggcagc tcccgcgcta
    21541 ttacgcaact cattctcaat ctggtagaag gttcaccaat taagctaatt gatggcggaa
    21601 aacaaaaacg ctgctttact gatatccggg acggtatcga ggcgttgtac cacattatcg
    21661 aaaatgcggg aaatcgctgc gacggcgaaa ttatcaacat tggcaatcct gagaacgaag
    21721 cgagcattga ggaactgggc gagatgctgc tggcgagctt cgaaaaacat ccgctgcgcc
    21781 atcatttccc accgtttgcg ggctttcgcg ttgtcgaaag tagctgctac tacggcaaag
    21841 gatatcagga cgtagagcat cgtaaaccga gcatccgcaa tgcccaccgc tgcctggact
    21901 gggagccgaa aattgatatg caggaaacca tcgacgaaac gctggatttc ttcctgcgca
    21961 ccgttgatct tacggataaa ccatcatgac caaagtaggc ttacgcattg atgtcgatgc
    22021 ctttcgtggc acccgtgaag gcgtgccgcg tctgctggaa atcttgagta agcataatat
    22081 tcaggccagc atttttttca gcgtcggccc ggacaatatg ggccgccatc tctggcgact
    22141 ggtgaagcca cagtttttgt ggaagatgct gcgctcaaac gcggcatcgc tttatggctg
    22201 ggatatttta ctggcaggta cggcctggcc aggtaaagag attggtcatg ccaatgccga
    22261 tatcattcgt gaagcggcta aacatcacga agtcggtctg cacgcctggg atcaccatgc
    22321 ctggcaagcc cgtagcggta actgggatcg gcaaacaatg atcgacgata ttgcacgcgg
    22381 tcttcgcact ctggaagaga ttatcggtca accggtaacc tgttctgccg ctgcgggctg
    22441 gcgtgccgac cagaaggtga tcgaagcaaa agaagcgttc catttgcgct acaacagcga
    22501 ttgtcgtggg gccataccgt tccgtccact gcttgaatca ggaaaccctg gcactgcgca
    22561 aattccggtg accttaccca cctgggatga agtgattggt cgggatgtga aagcagaaga
    22621 ttttaacggt tggttactca accgcatcct gcgagataaa ggcacgccgg tttataccat
    22681 tcatgcagaa gttgaaggct gcgcttatca gcataatttt gtggatctcc tcaaacgcgc
    22741 agctcaggaa ggcgtgacat tttgcccttt aagcgaactg ttatcagaga cgttgccgct
    22801 cggacaagtt gttcgcggaa atattgccgg acgtgaaggc tggctgggtt gccaacaaat
    22861 tgcgggtagt cgctgatgaa atcggtacgt taccttatcg gcatcttcgc gtttattgcc
    22921 tgctattacc tgttaccgat cagcacgcgt ctgctctggc aacccgatga aacgcgttat
    22981 gcggaaatca gtcgagaaat gctggcatcc ggcgactgga ttgtgcccca tctgttaggg
    23041 ctacgttatt tcgaaaaacc cattgccgga tactggatta acagcattgg gcaatggcta
    23101 tttggcgcga ataactttgg tgtgcgggca ggcgttatct ttgcgaccct gttaactgcc
    23161 gcgctggtga cctggtttac tctgcgctta tggcgcgata aacgtctggc tctactcgcc
    23221 acagtaattt atctctcatt gtttattgtc tatgccatcg gcacttatgc cgtgctcgat
    23281 ccgtttattg cattctggct ggtggcggga atgtgcagct tctggctggc aatgcaggca
    23341 cagacgtgga aaggcaaaag cgcaggattt ttactgctgg gaatcacctg cggcatgggg
    23401 gtgatgacca aaggttttct cgcccttgcc gtgccggtat taagcgtgct gccatgggta
    23461 gcgacgcaaa aacgctggaa agatctcttt atttacggct ggctggcggt tatcagttgc
    23521 gtactgacgg ttctcccctg gggactggcg atagcgcagc gggagcctga cttctggcac
    23581 tattttttct gggttgagca tattcaacgc tttgcactag atgatgccca acatagagct
    23641 ccgttctggt actacgtgcc ggtcatcatt gccggtagcc tgccgtggct gggattactc
    23701 cccggtgcac tgtacacagg ctggaaaaac cgcaagcatt ccgcaaccgt ctatttgttg
    23761 agctggacga taatgccgct gctgtttttc tccgtcgcta aaggcaaatt gcccacctat
    23821 attctttctt gctttgcacc tctggcaatg ctgttggcgc attacgcgtt gctggcagca
    23881 aaaaataatc ctctggcgct gcggattaat ggctggatta acatcgcttt tggcgtcact
    23941 ggcattattg ccacatttgt gatctccccc tggggaccaa tgaacacgcc ggtgtggcaa
    24001 accttcgaga gctataaagt cttttgtgcc tggtcgattt tttcgctatg ggcatttttc
    24061 ggctggtaca ccttaacaaa cgtcgaaaag acctggtctt tcgccgcgct ttgcccgctg
    24121 gggctggcgt tgctggtagg attttcaatt cctgacagag tcatggaagg aaaacatccg
    24181 caattttttg tcgagatgac acaagaatca cttcagccaa gccgctatat tcttactgat
    24241 agcgtcggtg ttgccgcagg tctggcatgg agcctgcaac gcgatgacat catcatgtat
    24301 cgccagacag gtgagttgaa atacggcctt aattatccgg atgcgaaagg gagatttgtc
    24361 agcggtgatg agttcgcaaa ctggcttaat caacatcgtc aggaggggat tattacactc
    24421 gtgctttcgg ttgaccgcga tgaagatatc aacagtctcg ccattccgcc cgcagatgcc
    24481 atcgttcgtc aggagcgtct ggtgctgatt cagtatcgtc ccaaatgatc tggctaacat
    24541 tagtctttgc cagcttgctt agcgttgccg ggcagttgtg tcagaaacag gcaacctgct
    24601 ttgtggcgat aaacaaacgg cgcaaacata tcgtgctgtg gctgggactg gcgctggctt
    24661 gtcttggtct tgccatgatg ctctggctgc tggtcttgca gaacgtaccg gtaggcattg
    24721 cctacccgat gttaagtctg aattttgtct gggtgacgct ggctgcagta aaactgtggc
    24781 acgaaccggt atcgccgcgt cactggtgtg gggtggcgtt cattattggt ggcattgtga
    24841 tcctcgggag tacggtgtaa tgtgcctgat atggggatta ttcagcgtca ttattgcctc
    24901 agttgcgcaa ttaagcctgg gttttgcggc aagtcatctg ccgccgatga cgcacttatg
    24961 ggattttatc gcggctctgt tagcttttgg cctggatgcc cggatactgt tactcgggct
    25021 gctgggttat ctgctttccg ttttttgttg gtataaaacg ctacataaac ttgcgctaag
    25081 caaagcctat gccttgctca gtatgagtta tgtgctggta tggattgctt caatggtact
    25141 tcccggctgg gaagggactt tttcgctgaa agcactactg ggagtagcct gtattatgag
    25201 cgggttgatg ctgattttcc tgcccacgac aaaacaacgt tactgagttt tccctgccgc
    25261 tgtacactgc cgttcccact catcggggct ataactgctt gccgagagga ctttcaccgt
    25321 atgcagcttt tcacgattaa tacaataaag cgcattctgc aaaggcgaga gtaaatcccc
    25381 cactttcacg gcgaagtcac tcttcacttc ggcgatcatt tttatcgcgc cgccagcatc
    25441 gcagagcata agtacatgtc tgttatcttg tttattgcag cacgattttt tgtgccagcc
    25501 attccattgc attatcctgt ttgctaagag ttttcacatc aatttacaga ttaacagata
    25561 aactctttat caacggcaat ggaatggcag aaatgagaag tgaaacggag ggttaattat
    25621 tgctgacgtt gcacccactc ttttagcgcc tgacgtgaaa ttttaatacc gccgtttttc
    25681 agctccggcg gtaaagtcag ccagcgcacc ggttgttgaa aacgagccag cttatctttc
    25741 acccattcgc caagatcaac ggtttgctgg tcatactcca ccaccgctac cggccgatga
    25801 ccaaactcct tgtcggcaac ggaaacgata aacacctgca aaaccgcagg atgtgcagca
    25861 ataacacgct cgacttcttc cggctgaata ccctcaccgc cactgaagaa cagattgtct
    25921 aaacgtccga caatggtcag cttgccatta tgcatctcac cgcgatcgcg cgtagcgtac
    25981 cagccttcgt cattaaccag tgaaaccagt tgcccgttac gccagtaacc ttctgccata
    26041 ctggcagccc gcagccacac ttcattatta acgattttca cttcccgacc cggcagcggc
    26101 aaaccaacgt ctgccaggcc gtcggcttct ttcgcacata ccgtggaggc aaactcggtc
    26161 agaccatagc cgcaaaagca acgaatcccc tgctcgcgcg cctgttccgt caactcgacc
    26221 gggatagccg cgccgccaag taacaccgct ttcagggaaa cggaactgcg gttaaccagc
    26281 aaacgccaga gttgtgttgg caccagtgaa gcgtgagtac agcctgccag catttgctcc
    26341 aatggctgtt tatcacgcac cgtcatccgc gcaccagcgt ataaccagcg ccacataatt
    26401 ccctgaccgg agacgtgaaa cagcggtaaa gagagcaacc agtcatcgtg atcgccaaac
    26461 ggaatcagcg ataacacacc ttgcgcactg gcaagatggg cctgataagt atggacagcg
    26521 gctttcggca aaccggtaga acccgaggtc aacgtcattg agcacagacg cgtcggctgc
    26581 cacgcagcgg catgtacgcc ttcaaccagc tgaatgtgca gcgacgttaa tgccggaaac
    26641 gtgttttccc catccggcac cagcgcaaat tgcagcgtca gattgggcag caattcttca
    26701 agcaacggtt gcggcagctg agggttcacg ggcaacaccc gcgccccgca ttgcagtaac
    26761 gccagccagg cgagcagcgt ttgcggcgta ttccacgccc gcaacatcac gccgctgccc
    26821 tcaaccaccc cctgcgccgc aaagcccgta gctaactcat cgacgcgagc acaaagctcg
    26881 cgccagttga gttgctcgtc attaagacgt aaggcgatgg cttctccccg cacttgccgc
    26941 cagtgacgcc acggccagtc agagaagatc ataacaactg ctcctgtaca tcagcgtcca
    27001 ctaacggcag tgggctcccc ggccagcgac gaacctgctg cgcctgcatc agatccagcg
    27061 tgtccagtcc cggaatggtg tctggcgtta accaggcagc aatccgcgcc agttgcgtta
    27121 gacctaagct cgactcaatg gaagaactga tcaccgccgt cagccccagc gcatgcgccg
    27181 cctgtacctg ctcgcgtact ttttccagac tgcccgtgag cgtgggtttg ataactaccg
    27241 cgcgcacgcc ctcttcagcc acaaaggcaa aatccggctc gcgcaggctt tcatcccagg
    27301 caatggcaat gccggtttca cgggcaaacg ctcgcgaatc atcgcgggtt ttgcacggct
    27361 cttcgagaaa cgcgatgcgg tggcgataat ccgggttaac atatttggca aactgctgac
    27421 ctttcagcgg tgtccaggcg cgatttgcgt caagacgcaa atgcagatcc ggaattgcct
    27481 ccagcaacag attcaccacc atgccgtcgc gcaccgcttc gtacaatccc actttgacct
    27541 tcgccacttt ctcgcccggc atatcggcaa gtttgaggat cagatcgtcc ggatcgccat
    27601 tacacagcgg tgccgcacgg tagttggctg cttgcggcaa cgtatctgtc agttctgcca
    27661 atgcacagct tacgccaaag gccacggaag gcatctgcgg tagctcgcaa tcgcctgcca
    27721 gccagttatt tacccaggca agcagcacgc tttgcgcctc ttcccagttt tcctggctga
    27781 agcccggcag tggggagatc tccccccacc cttcgcgctc gccctcacgc aggcaaacgt
    27841 acagcccgtc gcgggttttt aaccgcctgt cgcgcagaac cacccccgcg tccatgggga
    27901 tctgccagcg gtatacctgc gcgctacgca ttacggattc cgtttaaatt tgctgaaatc
    27961 aggctgacgt ttctggttga aagcgttgcg accttcctga ccttcttccg tcatgtagaa
    28021 cagcatggtg gcgttgcccg ccagctcctg cagccccgcc tgcccgtcac agtcggcgtt
    28081 cagcgcagct ttcaggcagc gcagcgccat cgggctgttt tgcagcattt cgcggcacca
    28141 acggacggtt tctttttcca gatccgccag cggtaccacg gtgttgacaa ggcccatatc
    28201 cagcgcctgt tttgcgtcgt actgacggca caggaaccag atttctcgcg cttttttctg
    28261 cccgacgata cgagccatgt aggaagcgcc ccagccgccg tcgaaggaac cgactttcgg
    28321 gccagtctga ccgaagatgg cattatctgc tgcgatagtc aggtcgcaca tcatgtgcag
    28381 aacgtgacca ccgccgatgg agtagccagc caccatcgcg acaaccggtt tcggacaggt
    28441 acggatctga cgctggaagt ccagcacgtt caggtgatgt acgccggaat catctttata
    28501 gccgccgtaa tcaccacgca ctttctggtc accaccggag cagaacgctt tatcgcctgc
    28561 gccagtcaga atgatcacgc cgatattgtc gtcataacgc gcatctgcca gtgcctggat
    28621 catctctttt accgtcagag gacggaaggc attgcgcacc tgcggacgat taatggtgat
    28681 ttttgcgata ccgtcggtgg atttttcata acgaatgtcc tcgaaacctt cggagcagtc
    28741 gtgccattca accggtgcgt aaggcattgc ttcatcagga taaatcatat tgtgtccttt
    28801 agtcagaaac gcaagatctg cgccagactt gcgattacgc cagcgggatt ttcccgatgc
    28861 gcgttatgtc cggcgcgagg aatgacatgg cagtcggcag ccagttccgc cgccagggcg
    28921 cggaatttgc tgtcacgttc accacataaa taataaaacg caaatgtgcg ggcgctaagg
    28981 ttagcacgta aatcaggctg gacggcgaga gaagtcgcct ccagcatggc ggcaagggtt
    29041 gcgccattat tgttgctgcg cagcgccacc agctcccggc gttgatcgtc attgagcgag
    29101 gcaaaaacag gctgttgata ccagtcggca aataccgccg ttaacggttc tgtgcgaaaa
    29161 cgctgcgccc attggcgatc ggaacgctga cgttccgcac gttgttcagc attttgcagc
    29221 cccggatgcc cgccttcgac aataacccca caaagccccg ccagcccctg gcaagccgcc
    29281 atcatcgcca cccgtccgcc aagcgagtac cccaccagcc agaagtcaag gatgttgtaa
    29341 ctaaccaagg ttttacgcag taagtcggtg acatcatcaa atccatcgac gctaatcgcc
    29401 gccgaaccac catgacctag gagatcaaca tacaaccgtg agtagtcggc aaacgcctcg
    29461 cccacttctt gccattcgtg gcaatcgccg gaaaaaccgt gcaaaaacac cagccagggt
    29521 aaacctagtt ttccgtgttt tgcctgcgcg tgcaggatca taaatggctt acctgcgcca
    29581 gaagttgctg gagcgtttgc gcaccatcgg tgtcgttaac caccatttca atcaccgtgg
    29641 tggttggcgt gcgccaggcg tcggcaaatg ccgtttcaag ttcctgccag ttttgcggat
    29701 gatgatattt cagctcgaac atcgcggcgg cgtgctcaaa atggacgttt tgcggcatca
    29761 gatagaaacg ctcacgctcg ctttgcggcg ttggcaacag cgagaaaatt tgcccgccgt
    29821 tgttgttcac cacaattaat accagcggcg cagaaacctg acgcaataac gccagcgcgt
    29881 tgagatcgta aagcgcggag agatcgccca caatcgccag cgtcggtttg ccgcttgccc
    29941 gctgaacgcc tgcggcggtc gaaagcagtc cgtcgatacc gctggcccct cggttgctgt
    30001 acaccgggta acctgccgga agttgcgaaa gcgcatcaat cagacgtacc accaggctgt
    30061 taccaacaaa caattgcccc tgttcaggca gatagtcgct gatgcgatgc gccagttgcg
    30121 cttcgccaaa cgcatcacgg cgggcaataa ccgcctgcat tgcctgttcc gccaggcgcg
    30181 ggatttcaac gcaccagggc tggcgttttt ctgccggatg cagctccagc cagtcggcaa
    30241 tattggcaat taagcgacgt ccgcgatggt gtgccggatc aagtcgccct tcaatgtcat
    30301 caacaatcca gtactcttct ggttcacagc ttgcctgcca ttgcaggagc cgtttccccg
    30361 tcaggctact tcccagttgc accacaattt gcgcctgctg cagctcgctg gtcgctttgg
    30421 cattgcctaa ccaaagatcg gcacacggca gcggctgccc ggtttgtgac agcacatcgc
    30481 caatcagcgg ccagccaaga gtttgcgccc acagggcaac ttttttgccc tcttccgcac
    30541 tcatgcgccc ggcaaccacc acgccgcgct tttgtcgcca gaagaaccag tcgcgctgtt
    30601 tttcactttc cagacgaggc gcttcacgca gccacggttt gtcgtcctgc caccagtcac
    30661 ccagacgctg ttgccagcta agcccggtat cgtccatttc gccatacagc ggttcagcaa
    30721 acgggcagtt gatatggact ccccccgcat gaagcgtacc gagagcgtgg tcgatggtag
    30781 aaaccagcca acgtgcgggg atatcctggg tcgggcgcgg caatgaaata ctgtgcgtgg
    30841 ggtgagaggc gaacattccc ggctggcgaa ttgcctgatt cgcgccgcag tcaattagct
    30901 ccggcgggcg atcggcggtt aagagaatca gtttttctcc ggttaacccg gcttcaatca
    30961 gtgccggata gagatttgcc accgccgtgc cggaggtcac aatcaccgcc accggctgct
    31021 tgctgacttt cgccagcccc agcgccagat gccccaaccc acgctcatcg aaatgggtgt
    31081 ggtgaatgaa tgcggaattc tccgccgccg ctaacgttaa cggtgtagaa cgcgagcctg
    31141 gggcgataca gatgtgtctg acgccgtgac gcgttaatgc ttccagaatg accgccgccc
    31201 agcgtcggtt aaatgcgctt actgacatga gtttgtccgg tatcaatatt gcggctaagt
    31261 ataaggagct acaaaaatag attattgata tgaatcggta atgatgcgac tcattactat
    31321 tccatttgta ataaagtacg cagccctgcc gctttgttgt cgatttcctg ccactcttgc
    31381 tcggggtcgg aaccacggac aatgcccgcg ccagcatata atcgcacgac attgccgcta
    31441 atttttgctg agcgcaggga aacgcagaat tcgctttgtt gtaatgagag atagcccgca
    31501 gaaccggcgt accattctcg ggtgaacggt tcgtgacggg cgataaactg tcgcgccaga
    31561 tcgcgcggta agccagcaac tgctgccgtt ggcagcaact gatgtaaaca gatcacatca
    31621 tccgctttgt tgagtgaagt ccagatacag cggcgaagat gctgcacttt acgcagacgc
    31681 agtacctgcg gcggtaaaac atccagcgtc tgggtatcgg cctgtaatcg ttggcagata
    31741 tcttccacca ccagcatgtt ctcgcgctgg tttttatcat ccgccatcag ccactctcct
    31801 aactgctgcg cctgcttatc atcaggatga tttgctactg ttcccgccag cgcttcagta
    31861 cgcagcgctt tgtcacgccg ccgccataac cgttccggtg aagagccaag aaaagcattt
    31921 tcgccatcaa aggccatgta aaaatggtag caattcagat tcagtcgacg actggcagcc
    31981 atcatcgccg ccgcgttgac cggacttgcg aaatgcaggt cagttgcccg agcgagcacc
    32041 actttgtcga gctcaccttc ggcgatggtt ttcgttgcca gttcgattaa ttgcgtccag
    32101 cccgttttgt ccggccagtg ttgttctcgc gtggtggtta aatgtaaccc aggcaagggc
    32161 ttgatactca ccagtgtggc gataaattct tttgcctgaa tcgcatcgtg ctgaagggag
    32221 ctttcgctga atagcgtcag ccgcagcgtg gctttaccgc cacagcgtcg ccattccagg
    32281 cggggtaaaa gtaaattgcc ctgcgacggg tcaaaagcat tcagccccca aatgcgtaag
    32341 tcagcgtgtt ccgggtgctg gcgaagaaaa cgttgtgcct ggtccaacga cgtaaaacgg
    32401 gtaatcgcgc ccaggacggc agcttcttca tcaccattac gttgttgcca gtagaattgc
    32461 gggtacgttt gctgactggc cagccagctc aaggcatcaa aagcgtcttt gagagggaaa
    32521 ggaatatcga taacccgaat gccgggtgtc gccggaatct cttgcgacaa atggcgcagt
    32581 agattttcca gcgccgtagt aagtgattgc acgcggacct ctccctgtta aaagcctcac
    32641 attatacggg gtactacaaa aaaaatgcag taccccggtg tagggaggtt ttaacggcgt
    32701 gccagcaaca gtcctagtac cagcccgacg gccgcgccca caccaattcc ttgccagggt
    32761 ttttcgtgga cgtagtcatc agcacgataa acagcctgct tcgcccgata gtaataactg
    32821 tctgaagcct ggctaacccg ttttttcaca tcatccagcg ctttttctgc atgcgctttc
    32881 agctcaacat atttctgatc ggcgggatcg ccagaggagc ggagcacctc ttccagtgtt
    32941 tcactaagca gcgtcaggtc gtcatcgata cgtgtatcac caaactgatt agacattctc
    33001 gttctccatt tgcgtaaaac ctgtcactaa ctatagacaa gagttgttga ttacgcctga
    33061 attacctcgc gtgccatccc aatgtgtggg ataccatctt cttcatagac ctccgtcaca
    33121 gggataaaac cgaaactctg gtagaagttt tgcagatgcg cctgcgcccc gaggtaaaca
    33181 ggcttatcag gccagtgatg cgtacatgtt tcgagtgttt tactcatcag ctgctgcccc
    33241 actttttcac cacgcaacgc ttcactgaca atcacccgac ctataacgac cggctcaaga
    33301 tcatcatcac ttttcagaat cctcgcatac gccaccagtt catcattttt ccaaccgaga
    33361 atatggcgat tatcccccgt caaatcatca ccatcgatat cctgataagg gcaattctgc
    33421 tcgacaacaa acaccgcgca acgcagttgt aataaggcat aaagctggga aaccgataat
    33481 tcagagtgat gcagatcttg ccattcaatc ataatgctct ccttttagat gtatgcctta
    33541 tactagccgg aatatttttt gaaacgtgat gaactcatgg aattaatttt tttaggtact
    33601 tcagccggtg tgccaacccg cacgcgcaat gtcacggcaa tattgcttaa cctgcaacat
    33661 ccgacccaga gcggactttg gttgtttgac tgcggtgaag gcacccagca tcagctactg
    33721 cataccgcct ttaaccctgg aaaactggac aagattttta tcagtcacct tcatggcgat
    33781 catctttttg gtttacccgg cttgctgtgc agtcgttcta tgtcaggcat tatccaaccc
    33841 ttaacgattt atggtccgca aggtatccgt gaatttgtgg aaaccgcgct gcggattagc
    33901 ggctcatgga ccgattatcc gctggaaatt gtcgaaattg gcgctggcga aattctcgat
    33961 gatggcctgc gcaaagtaac cgcttatccg ctggaacacc cactggaatg ttatggctat
    34021 cgtattgaag aacatgataa accgggtgca ttaaatgccc aggcattaaa agctgctggc
    34081 gtgccgcctg gcccactgtt tcaggaatta aaagcgggca aaacaatcac gctggaagat
    34141 ggaaggcaga ttaacggcgc agattactta gctgctccag tgccaggtaa agcgctcgct
    34201 attttcggcg ataccggccc ctgcgatgcc gcacttgacc tggctaaagg tgtcgatgtc
    34261 atggtgcacg aagcgacgct ggatataacc atggaagcca aagccaatag tcgcggccat
    34321 agctctacac gccaggctgc gacactagcc cgtgaggctg gagtcggcaa gctaatcatt
    34381 acccacgtca gctcgcgcta tgatgacaaa ggttgtcaac acctgttacg tgaatgcagg
    34441 tcaattttcc cggcgactga actggcgaat gatttcaccg tgtttaacgt ttaactctgc
    34501 ttattattaa aaacagggtg aaacttgccc tgttatagca acccgcgcta catatatcca
    34561 cccacacagg catattgagc gatttttgat atttattcat ctccttacgc actatattca
    34621 gagcaataag ttgcctcttt tcatcttgat tcagcacaac ctttgcaact aaaaggagcc
    34681 catgatggtt acagttgtca gcaattattg tcaattatct caaacccaac tcagtcagac
    34741 atttgcagaa aaatttactg tgaccgagga attactgcag tctttaaaaa aaacagcgtt
    34801 atccggagat gaagaaagta ttgagttact gcataatatt gcgttaggtt atgatgaatt
    34861 tggaaaaaaa gctgggtaat gctgccaact tactgattta gtg
//
LOCUS       DAJTSI010000026        34788 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000026 DAJTSI010000000
VERSION     DAJTSI010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34788
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.026"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..2098
                     /locus_tag="OO327_001153"
     CDS             <1..2098
                     /locus_tag="OO327_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCR6017774.1"
                     /translation="FNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNA
                     QGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLG
                     SFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQA
                     GKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNL
                     AISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYES
                     ISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSA
                     SRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTD
                     YRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYA
                     TVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDE
                     ESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAG
                     NRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLE
                     INGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQ
                     PKLRGNVQTAQREVAP"
     gene            2095..3183
                     /locus_tag="OO327_001154"
     CDS             2095..3183
                     /locus_tag="OO327_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR6017775.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="OO327_001155"
     CDS             complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="OO327_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCR6017776.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5054..6466
                     /gene="uxaC"
                     /locus_tag="OO327_001156"
     CDS             5054..6466
                     /gene="uxaC"
                     /locus_tag="OO327_001156"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR6017777.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6481..7968
                     /gene="uxaA"
                     /locus_tag="OO327_001157"
     CDS             6481..7968
                     /gene="uxaA"
                     /locus_tag="OO327_001157"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR6017778.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8051..8602
                     /locus_tag="OO327_001158"
     CDS             8051..8602
                     /locus_tag="OO327_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR6017779.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="OO327_001159"
     CDS             complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="OO327_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR6017780.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10447..11412)
                     /gene="alx"
                     /locus_tag="OO327_001160"
     CDS             complement(10447..11412)
                     /gene="alx"
                     /locus_tag="OO327_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR6017781.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11683..12681)
                     /locus_tag="OO327_001161"
     CDS             complement(11683..12681)
                     /locus_tag="OO327_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6017782.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12760..13452)
                     /locus_tag="OO327_001162"
     CDS             complement(12760..13452)
                     /locus_tag="OO327_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR6017783.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13529..14032)
                     /locus_tag="OO327_001163"
     CDS             complement(13529..14032)
                     /locus_tag="OO327_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR6017784.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14117..15253
                     /gene="rlmG"
                     /locus_tag="OO327_001164"
     CDS             14117..15253
                     /gene="rlmG"
                     /locus_tag="OO327_001164"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR6017785.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     LGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15538..15852
                     /gene="higB"
                     /locus_tag="OO327_001165"
     CDS             15538..15852
                     /gene="higB"
                     /locus_tag="OO327_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR6017786.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            15849..16265
                     /gene="higA"
                     /locus_tag="OO327_001166"
     CDS             15849..16265
                     /gene="higA"
                     /locus_tag="OO327_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR6017787.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16310..18328)
                     /gene="fadH"
                     /locus_tag="OO327_001167"
     CDS             complement(16310..18328)
                     /gene="fadH"
                     /locus_tag="OO327_001167"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR6017788.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18554..20905)
                     /gene="ygjK"
                     /locus_tag="OO327_001168"
     CDS             complement(18554..20905)
                     /gene="ygjK"
                     /locus_tag="OO327_001168"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR6017789.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20922..21341)
                     /locus_tag="OO327_001169"
     CDS             complement(20922..21341)
                     /locus_tag="OO327_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6017790.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(21319..21672)
                     /locus_tag="OO327_001170"
     CDS             complement(21319..21672)
                     /locus_tag="OO327_001170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6017791.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(21673..21993)
                     /locus_tag="OO327_001171"
     CDS             complement(21673..21993)
                     /locus_tag="OO327_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6017792.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(22127..23560)
                     /locus_tag="OO327_001172"
     CDS             complement(22127..23560)
                     /locus_tag="OO327_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6017793.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23623..24072)
                     /locus_tag="OO327_001173"
     CDS             complement(23623..24072)
                     /locus_tag="OO327_001173"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR6017794.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24069..27155)
                     /gene="ebgA"
                     /locus_tag="OO327_001174"
     CDS             complement(24069..27155)
                     /gene="ebgA"
                     /locus_tag="OO327_001174"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCR6017795.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="OO327_001175"
     CDS             complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="OO327_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR6017796.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28539..28870
                     /locus_tag="OO327_001176"
                     /pseudo
     CDS             28539..28870
                     /locus_tag="OO327_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(28912..30291)
                     /gene="ygjG"
                     /locus_tag="OO327_001177"
     CDS             complement(28912..30291)
                     /gene="ygjG"
                     /locus_tag="OO327_001177"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR6017797.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGVFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30709..32229
                     /gene="aer"
                     /locus_tag="OO327_001178"
                     /pseudo
     CDS             30709..32229
                     /gene="aer"
                     /locus_tag="OO327_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32383..33006)
                     /gene="nfeR"
                     /locus_tag="OO327_001179"
                     /gene_synonym="yqjI"
     CDS             complement(32383..33006)
                     /gene="nfeR"
                     /locus_tag="OO327_001179"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR6017798.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33283..34028
                     /gene="nfeF"
                     /locus_tag="OO327_001180"
                     /pseudo
     CDS             33283..34028
                     /gene="nfeF"
                     /locus_tag="OO327_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(34082..34157)
                     /locus_tag="OO327_001181"
     tRNA            complement(34082..34157)
                     /locus_tag="OO327_001181"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34122..34124),aa:Ile,seq:cat)
     gene            34282..34788
                     /gene="mug"
                     /locus_tag="OO327_001182"
                     /pseudo
     CDS             34282..34788
                     /gene="mug"
                     /locus_tag="OO327_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8483 a         8945 c         9142 g         8218 t
ORIGIN      
        1 tttcaacggt tatgatgcag atgatgtcag tgacagtaat actgacgtca gcgatctcta
       61 ttatcgttat gattttttac gtcgttatta tgtgcaggcg ggtcgcatgg acaaccgcac
      121 actatttaat gcacaaggcg ggaactttac ctttaacttt ctgccactcg gtgcaatcga
      181 tgggatgcgt atcgggtcga cactgagcta tttaaaccag gcgcaaagcc agcaaggaac
      241 cccagtaatg gttctgcttt cgcgcaattc tcgtgttgac gcttatcgta atgagcaact
      301 tttgggatcg ttttatctca atagtggttc gcaatttatt gataccagtt cctttccgcc
      361 aggcagctac agcgtggcat taaaagtcta cgaaaataac caactcaccc gcaccgagct
      421 tgtgccgttt accaaaaccg gcggtctgac tgacggaaat gcgcaatggt tcttacaggc
      481 aggtaaaact acatcacagg tttctgatga tgaaagttca gcttatcagc taggagtacg
      541 cctgccatta catccgcaat atgagctcta cgcagggctg gcgaatgctg atgatgtgag
      601 tgctttcgag ttaggtaata actggacggc agatttaggc agggtgggga atcttgcaat
      661 cagcgccagc gtgttccgta acgatgacgg cggcaaaggt gatatgcaac aggccaactg
      721 gagtaatccg ggatggccga cgttgggctt ttatcggacc aactctgacg gcgatgcttg
      781 tacaaccgac agcagagaga gctataacgc cttaagctgt tatgaaagta tttccgcgac
      841 agtttcactg aattttgtcg gctggaatat gatgctgggt tatacctgca cacaaaataa
      901 cactgatgat agtttgcgtt gggataaaca gcagagcttt gaaaataact atcttcgcca
      961 gacaacagcg caaagtatct ctgaaactgt acaacttagc gcttcccgcg ctattgtgat
     1021 gcgtgactgg attttgagta cgtcagtcgg tgttttccat cgtaatgaca acggtggcga
     1081 taacgacgac aacggcttgt acttatcgtt ttcgttatct gacacgccaa cgatggatag
     1141 caataacaac agccattcaa ccaatgtgcc tacggattat cgttatagcg aacaggatgg
     1201 cgatcaaacg tcatggcagt tatcgcatac cttttataac gattcattca gccataaaga
     1261 acttggcgta accgtcggag gcctgaacac cgataccata aacagcgcgg ttaacgggcg
     1321 ttgggatggg caatacggaa atgtctacgc taccgtatct gatagttatg accgtaagaa
     1381 tcatgatcat ctttcggcct ttacgggcac ttatagctct acgctggctg tcagtcgcta
     1441 tggcgttaat ttgggagcca gtggtacaga cgatttgctg ggtgcggtac tggtggatgt
     1501 gaaaggcttc tctgaacagg atgaagagag tcaggatctg caactcgaag cgcgggtggc
     1561 aggcagccga actttgcagc ttggtcaaag tgacagtgtg ttgttccctt atcctggatt
     1621 tcagtctggt tttgttgagg ttaacgacag tagccagggc aatcagcagg ggacaacaaa
     1681 catcattaac ggtgcaggga atcgtgaatt aatgttgttg cctggcaagc tgcgctatcg
     1741 cgaagtgtct gccagcttta actacaacta tatcggtcgt ttattactgc cggcagcggt
     1801 gaaaaaattc ccgattgtgg ggctgaacag cgccatgtta ctggtagctg aagatggcgg
     1861 atttacactt gaaattaacg gcagcgaaaa agagctctat ctgctttccg ggcagcaatt
     1921 ccttaagtgt ccactgagtg ttgtaaagaa acgcgccagc attcgttaca gcggagatgt
     1981 tacttgtagt gtggtgactt attcacaatt accggagtcg attcaggttc aggcacagtt
     2041 gaaacagcct aaattacgtg gaaacgttca gacggcgcaa agggaggttg caccatgaga
     2101 aatcgattga ttgcggcgat attgggtttg tttggcacgc tcactggcgt tcaggcagct
     2161 cctgacgtga ccagtgaaat tacgtatgat ttggcatctg gtagagcgga ttattacttc
     2221 tggaaggatg aagcgtcggc aggaaataat ggatatatgt ggtatgaatg ttcgtatcct
     2281 gacctccaac aaacctgtac agctaatgga aatatatcga cagtacaaat ctatttaact
     2341 gaacaacgca gtgggatgcg ttggccggta aaactcaaag gatttaaaac agccattgta
     2401 agtagcgatg aagcgccgct aggatgcaag gggggcaaag ggcttcagac gaatcttaag
     2461 gattctaata gatcttcatg tacagaagat ggtcaacatt attatatata cgatacaaag
     2521 tttcttacgc tctaccttga gcagacagag atgaagaatt tgccgattgg tggcgtctgg
     2581 aaggggaaag ttaaattaca ttcgaacagc ccggcccagg actatttcgc aaatattacc
     2641 ctgaatacgc tcgaccccaa ccatattgac gtgttcttcc cggagttcgc ccacgccacg
     2701 ccaagggtgc agttagactt gcatccaaca ggaagcgtta acggcagcaa ctacgcgcaa
     2761 gatctgacca tgttggacat gtgcctgtac gatggtttta acggtaatgc catcagttat
     2821 gaaatcatgc tcaaagatga agggcgacca gctgcagggc gcagagacgg ttacttctct
     2881 atctatcgtc agggagggac caccaccgac gagggagaac gcattgatta ccgggtcaaa
     2941 atgtacaacc cggaaaccgg tgggcaaatt gatgtgcgca ataatgaaaa tatggtctgg
     3001 aacagcatta acctgaaacg tgtgcgtccg gtggtactgc ccggtattcg ctatgccgtg
     3061 atgtgtgtgc cgacaccctt gactctggca gtcgataaat tcagcgtgat ggataaacag
     3121 gccgggtact acatgggcaa attgtcagta atctttacgc cttccttgcc aaccatcaat
     3181 taaccctgca aaccggacaa acgctttttt ctatataaac gacgaagccg cctgataagg
     3241 cggcttcgtc gttataactt acgttaaacg ttaatgttgc ggtgcgggat cgttatgtgt
     3301 ttcctgcgcc acctcaatcg ccggtttgtt ctgcaatacg gtccagataa ccagcgcacc
     3361 cagcaggtca aatactgcca gaactgcgaa cagcgggctg aagccgatgg tgtcagccag
     3421 tgcaccgaca accagcgcaa acagcgtact tgccagccat gcggacatcc cggttaaacc
     3481 gttcgccgtc gccacttcgt tacgaccgaa cacgtcagaa gagagcgtaa tcagcgcgcc
     3541 agacagtgcc tggtgggcaa aaccaccgat acacagcagc ataattgcga catacgggtt
     3601 ggtgaacaga ccgatcatac ccgggccaat catcagcaca gcacccagcg ttacgaccat
     3661 cttacgggaa acgatcaggt tcacaccaaa ccaacgctgg aacagcggcg gcaggtaacc
     3721 accgaggata caaccgaggt cagcaaacag catcggcatc caggcgaaca tcgcgatctc
     3781 tttcaggtta aagccgtaaa ctttaaacat gaacagcggg atccacgcgt taaaagtacc
     3841 ccaggccggt tctgcaagga aacgcggcag tgcgataccc cagaactgac ggttacgcag
     3901 gatctgacca acggacattt tcttcgccgt gctcacctgg tgctgggctt cctgaccatt
     3961 aataatatag tcgcgttctt catcggtcag atgcttctgg tcgcgcggat gtttatagaa
     4021 aatcagccat gccatcgccc agataaagct caatgcaccg gagatgataa atgccatctg
     4081 ccagctgtgc atcacgattg cccataccac cagcggcggc gcaatcatcg caccaatcga
     4141 agaacctacg ttaaagtagc ctactgcgat ggaacgctct ttcgccggga accattcgga
     4201 gctggctttc agacccgccg ggatcatcgc tgcttccgcg gcaccgaccg caccacgagc
     4261 aacagctagg ccaccccagc tacctgccag cgcggttgca ccacagaaca cggcccacag
     4321 cacagcaaac attgcataac cgattttcgt acccagcaca tccagtacat aacctgctac
     4381 cggttgcatg accgtataag cagcagaata ggctgcgatg atataggaat actgttgggt
     4441 ggagatgttt aactcttcca tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag
     4501 gtaaccaagc acggtgccga gcgtcaccag tgcgatcata taccaacgta accctttaat
     4561 tttacgcatg taaaacctca tcgttttgtt atatccgcag gaagcgcggc catcatcccg
     4621 ccagaggctg aagatgttag ttacgaccgg gtaaccgaaa aaaaaaaccg gcacgccccg
     4681 aacgttgcca tgaaagtttc gtgctaattc ggcttccgta ccggttaacc cgattcatct
     4741 aacaaatgaa tgaatcggca atttgcattc cgtcgactta tgatttgcga cggcagaaag
     4801 ataacttgtc atacaacctt aaaaggtgag agccatcaca aatgtgcgaa tatttgtagg
     4861 gacattacct gacgacagcg aggccagtac tggcgcggcc tgcagcgaga tttaccactt
     4921 tgagagtaat ttttttaact acgtttattg atctaactca cgaaaatatc ttcggactct
     4981 ggaaattggt gtgataactt tgtcagcatc gcaccataag caagctagct cactcgttga
     5041 gaggaagacg aaaatgactc cgtttatgac tgaagatttc ctgttagata ccgaatttgc
     5101 ccgccgtctg tatcacgact acgcaaaaga ccagccgatt ttcgattacc attgccattt
     5161 gccgccgcag cagattgcgg aagactatcg ttttaaaaac ctgtatgaca tctggttgaa
     5221 gggcgatcac tacaaatggc gcgctatgcg taccaacggt gtggctgagc gtctgtgtac
     5281 tggtgatgcg tctgaccgtg aaaaatttga cgcctgggcg gcgactgttc cgcacactat
     5341 cggcaacccg ttataccact ggacgcacct cgaactgcgt cgtccgttcg gtatcactgg
     5401 caaattgctt tctccgtcaa ctgccgatga aatctggaac gaatgtaacg aactgctggc
     5461 gcaggataac ttctccgcgc gcggcatcat gcagcagatg aacgtgaaaa tggtcggcac
     5521 caccgatgac ccgatcgatt ctctggagca tcacgcagag atcgccaaag acggctcttt
     5581 caccatcaaa gtgctgccga gctggcgtcc ggacaaagcc ttcaatatcg aacaggcgac
     5641 ctttaacgac tacatggcga agctgggcga agtttccgat accgacattc gccgctttgc
     5701 tgacctgcaa actgccttga ccaaacgtct ggatcacttc gccgctcacg gctgtaaagt
     5761 gtctgaccat gcgctggacg tggtgatgtt tgctgaagcg aacgaagcgg aactggacag
     5821 cattctggcg cgccgtctgg ctggcgaaac cctgagcgag cacgaagtgg cacagttcaa
     5881 aactgcggta ctggtgttcc ttggtgccga atatgcacgt cgcggctggg tacagcagta
     5941 ccacattggc gcgctgcgta ataacaacct gcgtcagttc aaactgctgg ggccggatgt
     6001 aggctttgac tccatcaacg accgtccgat ggcggaagag ttgtctaagc tgctgagcaa
     6061 gcagaacgaa gaaaacctgc tgccgaaaac cattctctac tgcctgaacc cgcgcgataa
     6121 cgaagtgctg ggcaccatga tcggtaactt ccagggcgaa ggtatgccgg gcaaaatgca
     6181 gttcggttcc ggctggtggt ttaacgatca gaaagacggt atggaacgtc agatgaccca
     6241 actggcgcag ctcggtctgc tgagccgctt tgtcggtatg ctgactgaca gccgtagctt
     6301 cctgtcatac acccgtcacg aatacttccg ccgcattctg tgccagatga tcggtcgctg
     6361 ggtggaagcg ggcgaagcac cggcggacat caacctgctg ggcgagatgg tgaaaaatat
     6421 ttgctttaac aatgcgcgtg actacttcgc cattgaactg aactaaggtc tggggttgat
     6481 atgcaataca tcaagatcca tgcgctggat aacgtcgcgg tcgctttagc agatttggct
     6541 gaaggcacag aagtcagtgt cgataaccag actgttacgc tgcgccagga tgttgctcgt
     6601 ggacataaat ttgcgttaac ggatatcgca aaaggggcca acgtcatcaa atatggcctg
     6661 ccgattggtt atgcattggc ggatattgcg gcgggggaac acgtccacgc ccacaatacg
     6721 cgcacgaatc tgagcgatct ggatcagtat cgctatcaac ttgattttca ggatctgcct
     6781 gcgcaagcgg cagatcctga agtgcagatc tatcgtcgcg ctaacggcga tgtcggggtg
     6841 cgtaacgagc tgtggatcct gccaaccgtg ggctgtgtca acggcatcgc gcggcaaatc
     6901 cagaaccgtt tcctgaaaga gaccaacaac gccgaaggta ccgacggcgt gttcctcttc
     6961 agccacacct atggctgctc acaactgggc gacgatcaca tcaacacccg caccatgctg
     7021 caaaacatgg tgcgccaccc gaacgcgggc gcagtgctgg tgattggtct gggctgtgaa
     7081 aacaaccagg ttgccgcatt ccgtgaaacg ttgggcgata tcgatcctga acgcgttcat
     7141 ttcatgatct gccaacagca ggatgatgag atcgaagccg ggatcgagca tttgcatcag
     7201 ctgtataacg tgatgcgcaa cgataaacgc gagccaggca aactcagcga actgaagttt
     7261 ggtctggagt gcggtggttc tgacggtctt tctggtatta ctgctaaccc gatgctgggg
     7321 cgtttctctg actacgtgat tgctaacggc ggtactaccg tactgaccga agtgccggag
     7381 atgtttggcg cagagcagtt gctgatggac cattgccgcg acgaagcaac gtttgaaaaa
     7441 ctggtcacca tggtcaacga cttcaaacag tactttattg cccatgatca gccgatctac
     7501 gaaaacccat caccggggaa caaagcgggc ggtatcacca cgctggaaga caaatcactt
     7561 ggctgtaccc agaaagcggg ttccagtgtc gtggttgacg tgctgcgtta cggcgagcgt
     7621 ctgaaaacgc cagggctgaa cttgttaagt gcgccgggta acgatgccgt agcgaccagc
     7681 gccctggcgg gtgcgggctg ccatatggtg ctgttcagta ctggtcgcgg cacgccatac
     7741 ggtggatttg tgccgacggt gaaaatcgcc accaacagtg aactggcggc gaagaaaaaa
     7801 cactggatcg actttgacgc gggtcagctg atccacggta aagcgatgcc gcagttgctg
     7861 gaagaattta tcgacaccat cgttgagttt gccaacggta agcaaacctg caacgagcgt
     7921 aacgacttcc gcgaactggc gatcttcaaa agcggcgtaa cgctataaag tcgaaatgag
     7981 ttgttaataa aaaacggcgt ttcataatgt gaagcgccgt ttgcttttcc atacctgcta
     8041 aggatttatc atgaccgcgt attggctggc ccagggcgtg ggtgtcatcg cctttctgat
     8101 tggtatcaca acatttttca atcgtgacga acgtcgcttc aaaaagcagc tttcggtcta
     8161 tagcgccgtt attggcgtac atttttttct tctgggcacc tatcccgctg gtgccagtgc
     8221 catccttaat gccattcgta cattgattac cttacgcacg cgcagcttat gggtaatggc
     8281 gatttttatt gtgctgactg gcggaattgg cctcgcgaag ttccatcatc ctgtcgaact
     8341 attgccggtt atcggtacga ttgtcagtac ctgggcgctg ttccgctgta aagggctcac
     8401 catgcgctgc gtgatgtggt tttcaacgtg ttgctgggtg attcacaact tctgggcggg
     8461 gtcgataggc ggcacgatga ttgagggtag ttttctgctt atgaatggcc tgaatatcat
     8521 tcgtttctgg cggatgcaga aaaggggaat tgatccgttt aaagtagaga aaacccctcc
     8581 cgccatagac gaaaggggtt aaacaattaa ttacgcaggg cgctatttgc cagacgatcg
     8641 tcttccgcct ggcaagctgc cgcagtgaac agcacgtcag ttgaagagtt cagcgcggtt
     8701 tcgcaagagt cctgcaatac gccgataata aagccgacgg caaccacctg catggcgata
     8761 tcgttcgaaa taccgaacat attacaggcc agtgggatca gcagcaaaga accccccgcc
     8821 acgccggatg cgccacaggc acacagagaa gccactacgc tcaacagcag cgctgtgggc
     8881 agatcgaccg gaatacccag cgtattaacc gcagccagcg tcaacacggt aatggtgatt
     8941 gctgcgcccg ccatattgat ggtggctccc agcggaatag aaactgaata ggtatcgcga
     9001 tccagattca gcttttcaca cagcgccata ttcaccggaa tgttcgctgc agagctgcgg
     9061 gtgaagaagg catacacacc gctttcgcgc aggcacagca gcaccagcgg gaacgggtta
     9121 cgacgaattt tccaccacac cagcaatggg ttaaccacca gcgccaccag taacatacag
     9181 ccaaccagca cgaccagcag ttgcgcgtag ccccacagtg tggagaaacc ggtggttgcc
     9241 agggtagaag aaaccagccc aaaaataccg aacggtgcga agcgaatgac cagtttcacc
     9301 ataaaggtaa cggcattcga catatcattg accaggtttt tggtggtctc gttaccgtga
     9361 cgcagtgcga agccgaggcc gatcgcccac accagaatcc cgatgtagtt acctttcagc
     9421 agggcgtcga tggggttgga aaccatgctc attaccagtc cgcgcatcac ttcgacaatg
     9481 cctgacggcg gcgaaatatc acccgcgcta ctggacaagt gcagggtaga agggaaggca
     9541 aagctgaaga ctactgcggc cagagctgca gagaaggtgc ccagcagata gaggaacaaa
     9601 ataggacgga tattggtttt ctgcccgtgc tggtggttag caatagatgc cattaccagc
     9661 atcaacacca ggatgggggc aacagctttc agtgcgccga cgaacaaagt acctaacaga
     9721 ccaacagctt ccgccgcggg ttttgagatc catgccagaa gaatccccag aacaaggccg
     9781 accaggattt gttttaccag gctgccatga gccagacgcc ggaatagccc cggtgaacgt
     9841 tgcgtagtca tttttcgatc ctttcattgt gttgtcgcac atccctggtg cgttctgtcg
     9901 acgcatcttt caggatgtaa caaagtgttt gcaaggtcga gtataaggaa agttacagct
     9961 tcgggaagcg aaaaatgctg gattttaaag agaggtcatg ttttgcaact taatggttgc
    10021 atttatttaa cataaacaga gggaaggact ggctactaag gctgattaga taacatatgg
    10081 gcaaaactat ttggaatatg agaacggttg cgctccttta acgtgttatg ccatataaag
    10141 tcactggcgc agggagcgcg cagggggcgg ccaatcgccg ccgccccctg cacccccagg
    10201 ctctggcgaa caaaatcgcc gctgcgcggt tccctcagcc tatcccttgc ggctaccggg
    10261 tcgggcgcaa ggtaacgtcc ctgtaagatg cgccctcgcc ccggcctcca gggaacgctt
    10321 cggcgatttt gacgccacca acacccgtgc ggtctttttg taaagagcta taagccgatt
    10381 acataattaa tagctcacct ttgtctggaa tgagttatct ctaggaatgg cttaatatac
    10441 agcgaatcat tctccccgct gcttatcatg ccgataattc acccaggcgt tgataataaa
    10501 cgtcatcacc agaatgccaa acaccacgcc cagcgagacg gcgattggaa tatggtagaa
    10561 gtcgacaatc agcatcttga taccgataaa caccagaatc accgccaggc catatttgag
    10621 catcgagaaa cgctctgcta cgcccgccag caggaaatac atcgcacgca ggccgaggat
    10681 tgcaaacagg tttgaggtca gcacaatgaa cgggtcagtg gtcacggcga agatagccgg
    10741 aatgctatcc acggcaaaaa tcacgtcgct caattccacc agaatcagta ccagcatcag
    10801 cggtgtggcg tacagcaatc cattcttacg cacaaagaaa tgctcgttgt cgatggtgtc
    10861 ggtcatgcgc aaatgaccgc gtagccagcg caccagcggc ttgtcgccaa tgcctgattc
    10921 atcttcatgg gcgagcgcca tcttcacgcc ggtaaacagc aggaaggcac cgaagatata
    10981 caggatccag tcgaactgcg aaatcagcca gctgcctgtg aagatcatga tggtacgcag
    11041 aacaatcgcc ccgagcacgc catacaccag cacgcgacgt tgtaatgccg ccggaacgga
    11101 gaaatagctg aacaacatca gccagacaaa gacgttatcg accgccagcg atttctcaat
    11161 cagataaccc gtgagaaagg ccagggcctg tggatcggca acggcgcgac cttcggtttg
    11221 caccagatac caccagaaag cggcgttaaa cagtaacgac agcgtcaccc agaccagcga
    11281 ccaggccgca gcctgtttca tggtcatggc atgtgcccca cgacgcccct gcaacaacag
    11341 gtcgatagcc agcataatgg cgacaacaac agcgaatccg ccccatagca acggcgtgcc
    11401 gacagtattc atagaagttc cttacacata aaaaaacggc caacgtcgga agacgtcagc
    11461 cgctgctttt tatgcataga cctcgccttc cggcaaggtc tcacttacaa caaaaaagga
    11521 atacgtctgc gtattccttt cgggttgccc ggtgaccgga tgtggttttt cactcatcgt
    11581 aatgacgatc gaccggcaat gaagttactc ccctttgcag gtaacaaagt atgacagacc
    11641 attcagtcag tcaatggtct gttacatacc ttttacactg ttttactttg gcaagtatag
    11701 ttttacgcta tctgccggaa aaatcacccc agtttggcgg cggatctcgg tcagcagttt
    11761 ggctgtgatg cgactgaccg ccagccctgg atgatccacc agatgctcat ccaccagggt
    11821 agcgaacagc tctgcttcgt agagcatggt attaatatgc tgcggctggg tgagatcctg
    11881 catctggctg ccacgcggca caaagcagac tttctggcat tcagacagtt tttcaatcac
    11941 cagcgatccc gcttcgccct gaatctcgct tcccaggaca gaatcactga cctttgagtg
    12001 ctgcaaggtg acgctgaaat caccgtaatc catcaccacc acgccgtggg cgtcaacgcc
    12061 gcttgccagc aaactcgccg ttgcctgcac gcttttcggc tcaccaaata acgccaccgc
    12121 cgacgccagg cagtaaaagc cgatatccat aattgaaccg ttagagaatg ccggattaaa
    12181 ggtgttggga ttctcaccgt ccaggtaacg ttgataccgc gaggaatact ggcaatagtt
    12241 gaaaaagact ttacgcagtt tgcctgcttt cggcagcgcc tggcgcaaca aatgaaagtt
    12301 cggcaggcag gcggttttaa atgcctcaaa cagcaccacc tgattttcgc gcgcacaggc
    12361 aatggcggca tccacttccg ccagattcga cgccagcggt ttttcgcaaa tcacatgaat
    12421 tttatggcta aggaaaagtt gtgtctggga aaagtgcagg gaattcgggc tggcaatata
    12481 caccgcgtca atggcatcgc tttccgccat cgcttccagc gacgtaaaca gatgctcgac
    12541 agaaaaatca ttggcgaagt gctgagcctg ctcaaggctg cgggaatata cggcggttaa
    12601 cttgtattta ccgctctcat gggcggcctc gacgaactgg cgagtgatcc agttcgtacc
    12661 aatcacagcg aaacgtatca taacgcgtgg atactccata aagggattct gtcgtcaatg
    12721 tagcacgcct ttttcatctt cgacgtgcgc agatacgcat tattctttcg ccggacagcc
    12781 atgctcacta aacggcccaa tcatgaccga tggcccgaga tatttcggct ggcgatggag
    12841 gatttttgcg tcaatcaccg cgctgacacg agcgagtttc tcccgcagtt gagtataaaa
    12901 accgtgcttc atattgaggt ctggcgcgtt aaacccgata gcgtcgatgc cgtactgttt
    12961 tgccagccag atggtgcgtt ggttatgaaa ttcttgcgag ataatggtga tatggttttc
    13021 cccaaacacc tttttggcac gcactaccga atccagcgtt gagaatccgg catagtcgca
    13081 aaagatcact ttcgccggta cgcctttagc gatcaaagcc tgctgcatac ctgatgcttc
    13141 atcgtaattt ttacgtccgt tatcgccact caccagtagc catttcactt ttccggcgtg
    13201 gtacaacgct gctgcggtat caatacgtcg ggtaaagtag cgattacccg gcctcgcccc
    13261 cagcaataaa cccacgttac gcgccgggac ggcgttgaca tcgccccagg tcagctgctt
    13321 gctggcgttg accataacgc gatcggcgat aaaaatggtc gctcctgcca caagtaacag
    13381 taagcaggca atctttagag tccggcgaga aaaacaaata cggagaagaa ggcgggcaaa
    13441 tgcgcgcagc atggtcgtta tccttaacga aaggggggag gtcgatttta ggcgctaaag
    13501 aaagaaaaga taacgcctga catcattttc agattttgtc ctgcccaagc gacaactgcg
    13561 ttagccacag ccgggtgtcg aactcaagct ggtggtactg cggctccata tggcagcaca
    13621 attgatagaa cgctttgtta tgctctttct ccttcaggtg cgccagctcg tgcaccacga
    13681 tcatgcgcaa aaacggttcc ggcgcgttgc gaaacacggt agcgacgcgg atctctgctt
    13741 ttgctttcag cttgctgccc tgcacacgag aaacagcggt atgtaacccg agcgcgtttt
    13801 ttagcacatg gatcttattg tcatacatca ctttattgat cggcggggca ttacgcagaa
    13861 actgattttt cagatcctga gtatattgcc agagagcttt atcggtggcg tagtcgtgtg
    13921 ttcccggata gcgttttgcc agcacatcgc ccagacgctg ttcgttaatc agtgttcgca
    13981 cctgcgaaag cagctgctcg ggataaccct gaagataagt aagattgctc ataaccgccc
    14041 acgaaaaaga gaaaaagggt atactcacgc acccttttca ggggcaacgt cgaaatttta
    14101 tcattcagga gggccgatga gccacttaga caacggtttc cgttcactga cactacaacg
    14161 ttttccggcg acggatgacg ttaacccgct acaggcgtgg gaagcggcag atgaatattt
    14221 gctgcaacag ttggacgaca cagaaatcct cggcccggtg ttgatcctga atgatgcctt
    14281 tggtgcgtta agctgcgcgc tggcggaaca taagccgtac agcattggcg actcatacat
    14341 cagtgaactg gcgacgcgcg agaatttacg cctaaacggg attgatgagt cgagcgtgaa
    14401 gtttctcgac agcaccgccg actacccgca acagccgggt gtggtgctga tcaaagtgcc
    14461 gaaaacactg gcattgctgg aacagcaact gcgtgcgctg cgcaaagtgg tcacgtcgga
    14521 tacacgtatt attgccggtg ccaaagcccg tgacattcac acttccacgc tggaactgtt
    14581 cgaaaaagtg ctcggcccga ccaccaccac gctggcatgg aaaaaagcgc gcctgattaa
    14641 ttgcacgttc aatgaaccgc cgctggccga tgcgccgcag accgttagct ggaagctgga
    14701 aggtactgac tggactatcc ataaccatgc gaatgtcttc tcccgcaccg ggcttgatat
    14761 cggcgcgcgc ttctttatgc aacatctgcc agagaatctc gaaggggaga ttgtcgatct
    14821 cggttgcggt aatggcgtta ttggtctgac gctgcttgat aaaaacccgc aggcgaaagt
    14881 ggtgtttgtc gatgaatcgc cgatggcggt tgcttccagc cgtttgaacg ttgaaaccaa
    14941 catgccagag gcgttggatc gcagcgagtt tatgatcaac aacgcgctct ccggcgtgga
    15001 gcctttccgc tttaatgctg tgctctgcaa ccctccgttt caccaacaac atgcgctgac
    15061 cgataacgtc gcctgggaga tgttccacta cgcccgccgc tgcctgaaaa tcaacggcga
    15121 gctgtatatc gttgccaacc gccatctgga ttacttccat aaactgaaga aaattttcgg
    15181 caactgcacc actattgcga cgaataataa atttgtggtg ctgaaagcgg tgaagctggg
    15241 acgtcgtcgg taagattggg agttacctgc cggatgcggc gtaaacgcct tatccggcct
    15301 acgttcagca cgttaccccc aggccggata agacgcggca agcgtcgcat ccggcaatgt
    15361 ctgctcagtt ctggaatcgc gcttccataa aacaaaaaat ccctgtcacc atcctctaca
    15421 ttctcttgtt tagcgttttt ctacgtttat tcttccgtca cacagataaa ttcatccgtt
    15481 gccaatctgt caacggatgt tatcatattg ccaatttgtc aacggacgtg atgaagaatg
    15541 cacctgataa ctcaaaaagc attgaaagat gctgcggaaa aatacccgca acataaaacg
    15601 gagttggtgg ctctggggaa cacgattgct aagggatatt tcaaaaaacc tgagtcatta
    15661 aaagcagtat tcccatctct ggataacttc aaatatctgg ataagcatta tgttttcaat
    15721 gttgggggca atgaattacg tgttgtagca atggtctttt ttgaatcgca aaagtgctac
    15781 atacgtgaag ttatgacgca taaagaatac gatttcttta ccgctgttca tcgtactaag
    15841 gggaaaaaat gattgcgatt gccgacatct tgcaagcagg agaaaagcta actgctgtgg
    15901 caccttttct ggcgggtatt cagaacgagg aacaatacac ccaggcgctg gaactggtag
    15961 atcatctgct gctcaacgat cctgaaaacc ccttgctgga tctggtgtgt gccaaaataa
    16021 ccgcgtggga agaatcagcg cccgaatttg cggaatttaa tgccatggct caagccatgc
    16081 ctggcggtat agccgtgatt cgtaccctta tggatcaata tggtttaacc ctttccgatc
    16141 tgccggaaat tggcagtaaa tctatggtgt cacgcgtttt gagcgggaag aggaaattaa
    16201 cgctggaaca cgctaaaaaa ttggcaacgc gattcggcat ttctcccgcc ttgtttattg
    16261 attaacgcga cgtgccgaat gcgttgcatc cggcgcttca aataacgatt taaatctcca
    16321 gcgccagccg tgttccctgg gcaattgccc gtcgtgcgtc cagctccata gccacatcgc
    16381 agccgccaat taaatgcaca gttttcccgc tatcaatcag cggttgcgcc agcgcgcggt
    16441 ttggctcttg ccctgcgcag atcaccacat tgtccactgc taatacctgc gtttcgccgt
    16501 tgatcaccac atgcagcccg tcatcgtcaa tcttctgata acttacgcct gggatcattt
    16561 tcacaccccg cgagagcagg gtggtgcgat ggatccagcc ggtggttttg cctaacccct
    16621 gtcccggttt gctggctttg cgctggagca tcacaatttg ccgtgggcta cgcgggatct
    16681 gcattccctg cgggcttaag ccaccagcct gttgtaggct gctgtcgatc ccccactcat
    16741 tacagaacct ggcgatattc tggctggtgg attcgcccgg ctgacttaaa tacatcgccg
    16801 tatcaaaacc aatcccgcca caaccgatga tggcaacctt gttgcctacc ggcgctttgt
    16861 cgcgcagtac atcgagataa ctcaatacct tcggatgatc gatcccgtcg ataggcggaa
    16921 cacgcggcac gatcccactg gcgaggatcg tttcatcgaa cgcctgtaac tgatccgccg
    16981 tcacggtgtg attgagtttc agcgtcacgc ccgtcacttc gatcatccgg cggtagtagc
    17041 gcagcgtttc gtaaaactcc tctttgccgg ggatctgttt ggcgatatta aactgcccgc
    17101 cgatctcgct gtgcgcatca aacaatgtta cctgatgccc acgcgccgcc gcgttaatgg
    17161 caaacgccag cccagcaggt ccagcgccga ccaccgccag attttttttc tgcacggcgg
    17221 gaaggattgg cattttggtt tcgtggcagg cgcgaggatt caccaggcac gaggtgactt
    17281 tgccaacgaa gatctgatcg aggcagccct ggttgcagcc aatacaagtg ttgatctcat
    17341 cggctcgccc tgattgcgct tttgacagca gttccgcatc cgcgagaaac ggtcgcgcca
    17401 tcgatactat atcggcatcg ccgcgcgaga gaatatcgtc ggcaacctgc ggatcgttaa
    17461 tccggttggt ggttaccagc ggcagcgaga cgtggccttt cagtttgcgc gtgacccagc
    17521 taaatgcgcc gcgcggcacg ggcgtggcga tggtcggaat ccgcgcttcg tgccagccta
    17581 ttccggtgtt gataatggtc gcgcccgccg cttcaatggc ctgcgccagt tctaccgttt
    17641 cggcaaaagt gccgccgcct tcgactaggt cgagcatcga caaccggtaa ataataatga
    17701 agtcgttgcc gacgcgttcg cgcaccgtac gcactacttc tacggcaaat cgcatccggt
    17761 tgcggtaatc gccgccccac tggtcactac gctgattggt gcgcagcgtc agaaattcgt
    17821 tgatcaaata cccttcagag cccatcactt cgacgccatc gtatccggcc tcccgcgcca
    17881 gttgcgcgca gcgggcgaaa tcgtcgatca gttgcaggat ctcttcatgg gtgagttcgt
    17941 gaggaacgaa acggttgatg ggggcctgca atgcggacgg tgcgaccaga tgcggttggt
    18001 agctgtagcg cccggtatgc aaaatttgca gggcgatttt gccgccttcc tgatgtacgg
    18061 cttcggtaat ggtgcgatgg tgtgggatct ggctggcgtc gttgagcatt gcgccacctt
    18121 ccatgccaac gcctgttaaa tctggtgcaa tgccgccgct gacaatcagc gccacgccgt
    18181 gacgggcgcg ttcggcataa aacgcagcca gccgttcagc accgtccggg tattcctcca
    18241 gcccggtgtg cattgaaccc atcaacacgc ggttttttaa cgtggtaaaa cctaaatcca
    18301 gcggggcgaa cagcgacggg tagctcataa aattgtccag tatgtaaaat aattgttatg
    18361 tagtcggatg agttctaatt tagccgtcga tggagaaaag ggaaaagggg agtgcggggt
    18421 ttgtgatggg attcaaaaag aatacgcccg gtggtaacgc cggatgcgac gcttgccgtg
    18481 ttttatccgg cttgggggat tgcacatgta gggcggataa ggcgttcacg ccgcatccgc
    18541 cagccgttgc aaattactgc ttacggaaaa aatcgttata caacatatac aaatgagcgg
    18601 cactccagga gaaatttggt gcgccttgct gcgcgcctgt cagcgggttg taattctcct
    18661 gaattgggcc atcggcggtt aatcctttgg cgtgcctgaa gaacgtatcc gccagcttca
    18721 gggcatcatc acgataaccg taacgctcca tccctttcag accaaaccag aactgatcca
    18781 cccatacgcg cccgcgccag tagatatcag cgccaaaagc cggattggtt aacgccgccg
    18841 ttcccagcgg aacaaatgtg ttgaactctt taggatcgag catcaccttc accaccgcgt
    18901 cggcattggc ctgcgttgcc gcaccgttaa acagcggcga ccagccttcc ggccctttac
    18961 cgcgctcaac aatcggtttg cccgcgcagc cgttcgccag tggtttatct tcaatacgta
    19021 cgtcatagta gaactgggta gtcgggtcga acatacaggt gttgatgtag tccgcgagct
    19081 gctgtgccaa ctggcgatag cgtttggcct cttctggctt accgaggatg gttgccatct
    19141 ccgccagata atggttatcg ctgtacatat agctggcctg atccaccgac tcctgcaata
    19201 gcgagtagcc cagcaacgtt ccgtcctgac tgcggttttc ggcgaatttc accgtccagt
    19261 cgctacgttt gccgccatta gcgacatatt tatccaactg ttctttgtcg ataaaaccaa
    19321 agacggcggc atcatcacgc cccgattccc acgacgcagc gacctgtgcc ggaatttcca
    19381 gactgtcgta ctggcctttc tccaccacgc gggcgtagtt gttcagccca gactgcgtct
    19441 cttctttatc gccttttttc accgtaaaca gcatctcgcc gctctcggtg ttgtgggctt
    19501 tgtcgcgggt cgcgccatat tccggcaccc cgttgccgtt atgatcgcgg ttacgtaacc
    19561 accagtcgtg ataggccacc agtttcgggt acatctctgc caaccaggct ttatcctggg
    19621 tgacgttgta tacttccatc accgaccagg cggcaaggct gggtttggta ttacgttcgt
    19681 tccagttgcc gccatcgcca ccacgctcgg ggctaaggtt ccacgctatc aggtcgggga
    19741 caaagcccac atcctgcgga cgcacgctgt cgccaggctg gatctgccag gagaagaccg
    19801 cgcggatatt ctctttggcg atatccggat tgaaatgcgc catcgcaaac gcctgcttcc
    19861 aggtatccca cggccaggtc tgattgccgg agaaccagcg tccggtcacc gacggcgtga
    19921 cggtgttata attcaccgca ccgcccggtg agcgccagtt accgttgagc gtttcgatcg
    19981 ctttcaccgc gacgcgcgtc tgttccggcg tcgcatccgg attggttaac cctttcttca
    20041 ggtattcttc ccagcgttgc tgcgaggcgg tgagataaaa cgccggacgc gccagaatat
    20101 cgcggatctg catttgctct ttgctaactt cctgagcggt cagcagatgg gaataggtgg
    20161 tgtagagcgt ggtcgaaccg ttgatatgcg ccttactggt aaagcgatta ccgttgattt
    20221 cagtctgcac cggcagggat ttatgcacct gatattccga ttcgccggag gtcagcagat
    20281 cccaggtggc gcgcactttg ccaaaggtga ctttcaggcc atcacgggtg gcgctgattt
    20341 tgcgctgata gtcagggtat tcgccagcaa tggttttatc ggaaagcggt ttcccttctt
    20401 tcgcttccag tttttccagc agttcgccat cccacaccag atccagcggt ttattgctga
    20461 tgattttagt ttccagtagt gacgtgcgcg gcgtggcgaa gcgcagagtc atttcgacct
    20521 gcacatcttt tgctgtcagt ttttgcacca gcgcgccggg aatactgtat gcctccagcg
    20581 tgaagttgac tttcttgccg tcttgccaga cagtcaggcg gtcgaaattg ctggccataa
    20641 agttgatgta ttcttccgtc agcagcgcaa cgcccggaaa gccgcccatg gtgttcgggc
    20701 cgtctggcaa cagatgacca tgccaggctc cgagatcgaa aaacggatta aaacgctggt
    20761 gatcgtcgta atcgtaatcc ttcatgtact gcggcgcacc agtacggtta atcacgtttt
    20821 tataattgtc ggcgttagcg gcatgagcgg aaaaacttat cagcagagcg caggttactg
    20881 gcgttaaaat agttttgatt ttcatgagat tacagtcctt attaccagta gaaatattgc
    20941 atcaggaaga tttggtcgct gggatcgtta gaggcatttt tcatgtagaa gcggctatca
    21001 aaagccgtgg cgaaacgtcc gccgccatat tcgtaccaga taccaaactg catgaaagag
    21061 gtggtttcat cgtcagcgtg gtcagggcga tgtttggcgt aggtgtaagc ggtggagaga
    21121 taaacgcctt ttaacgcttc atacatggcg ctcaccatca tcccgctttc acttcccggt
    21181 acgtccagcc cgtcggcacg tccggtatgg tgccaggcgc gagcggcaaa gttaggggta
    21241 aacaggcccg tgagatagag ttgtcctgtg ccgtcggtaa tttccagacc attcatccag
    21301 gtgatgcctt cggcaaggtc atactggatg taaccgttca tccattgccg gataggtgta
    21361 tttgtcgtcg tagcgatcgt acttgccaaa atgtagccag gctttgcttt cgtcatgatg
    21421 tccggcaggt gtggcggtga cgctataacg aaagttgccg cttaagttct gtaccttcag
    21481 cgcggtcata atgtcgcggg tattgggaat gacgtagccc aaatcgggtg tgaagtcgcc
    21541 ccaccattgc aggtcatcca gcgaggaatc ctgacgcaga ctgagcatga tttcggtgcc
    21601 atcattggta cgataaccgc catagaaacg gttaatccca ccttcgaaac caccccagct
    21661 atggtcaggc acctaggcat taccgtcgtg gtcggcctgc acggtccagc cttcgccgta
    21721 aatcaggccg aaccaggagc cgtacttcat ctccaggcca ccttcaatat aggtgccgtc
    21781 gctgtattta tgtttattct caccctctaa atccatatat ccaccaagcc ctaattcgcc
    21841 gtaaaaacga aatgaaggag aagatgtcga ggtaattgcg ggtgtttttg tgtcgtcatg
    21901 ttctgccgga cgtgcttctt ctcctgccgc agaaaaggcg tagcaaaacg gcagagcaag
    21961 taatgctctg catggtgcag tgattaattt cattgtgtta atccatgagc taaagaaaaa
    22021 gtacagcctt ttatcgttgt aattataaaa atataaaggc agaaactatt ttccctctcc
    22081 tcatccagga agagggaaaa tgcctgctca ttttttatta gtctgcttaa tcaacattgc
    22141 tggctggcgt cgcttcaata tggcgctctt cagccgttaa tccctttata tatttgctgt
    22201 atttccacca cgcaaagccg aggaagataa caataccgcc gacgttataa aaaatgatgg
    22261 tcagaatatt cgcgccagtc gggaatgtcg aagcgacaaa ccctacggca aatatcgcaa
    22321 tcagcattga aacaacgata ataccggtgc ggcgggagcc catacggaaa tcgcgtggca
    22381 ggtgatctaa tttggcgcgt aaattcaggt aagccagcat gatgaataac ggcggaagca
    22441 tggacgctgc ggcggtcata ttaataatgg tattcatcag atcctgcacg gtattggaac
    22501 cgagcatcgg gataatcatc agcgggatga cgatcagaaa ctggatccat gctgcgcgcg
    22561 ccggaacgcc gttttcgtta agttcgacgg ttttcttacc aaagatgcct tccgggattt
    22621 cggagaagaa aattttcacc ggcgttgcgg tccacatcag cagggaaccg aacattgcgg
    22681 taaaggacac cagaccgaca aagcgattca tcaacgcttc cggcagacca aaatacgccg
    22741 ccatgccgtg gaatacctgc accgatccgc cggtaaattt caactctttg ctgctgacga
    22801 agacgttaat cagcaccgag gagacggaat acagtacgcc gataaaaatc ccggcgagga
    22861 tgatcacttt aacgaacgat ttcgaaccgc ctttgacgtc gttaacgtac accgcgacgg
    22921 actccgcgcc acctgcggcc ataaagatcc aggtggtaac gccgaggaac gcccagttga
    22981 agttcgggat catcgcgtca acggtgatcg gatcggcagg ctgtacgccg ccaaccagtg
    23041 ccgtacctgc cagtaaaatg taggagagcg ttaacagcag catcagcgtt gaagtgacgg
    23101 aggtaattgg ccccagcatt ttcgccccgt tggtggaaac ccaggtggag aaggcgaaca
    23161 gcaccatact gataacggtg gtggcaaccg gcgtcataat atattcgtag ccgaggaagg
    23221 catacgaagc ataagcaata acgcgcggca acagcgaggt gaaaaagaac agatttacga
    23281 accagtaagt gtaggcagta ataaatgccc aacgtccgcc cagcgaactt tttacccacg
    23341 cgtagacacc ggcttctgag tttttattta acgaaacaaa ttctgcgatg atcagacaga
    23401 agggaataaa ataaaaaatc gtcgcgagga aaaacatcgg tgccgaggcc agtccaagct
    23461 caatattatt attgataacg ttgttaaagc tgtaaacggc ggcaaaagtc agcgagagca
    23521 agccgaattt gccgattgta ttacgtttgg tatcagacat aacacatctc cgaaaatgtg
    23581 tagggtagcg gtggcggcaa actccttttg ccgccgtagt tgttatttgt tatggaaata
    23641 accatcttcg atggtgactt tgagaaccac ttttttgacc gcgttattgc agataaaccg
    23701 atacgcttca tggatatcgc aaataacgat ttgcccttcg tggacctcta cggtttctcc
    23761 gcagcctttt aaatattcac ggtcagtttc atcacgataa tattccacta cctgtaatgt
    23821 ctctttcggc gcatattcaa ttttttgctg cccttgcagg tagtaatgca cttcaaaata
    23881 gcgtcgatgc ccggtaaata gcgcatcggt cgcggagtca ttttccacac ggtaggtcaa
    23941 tgaatcaccg atggagtggg cgacgccagg ctgaatgttg tcgatatttt caatcgcttc
    24001 aacgcagcgt tgccacttct tgccagaggc gtaaatctgg cggaactgtt ctaagttatc
    24061 gatgatcctc attgcttatt ctcgctgtgc aaattcgtgg aaaagaaccc tgcgccgaac
    24121 tcatacgacg ccaggctttg cgcggtagct tctccgccag aaaccggcag caacgtaaag
    24181 ccgtagctga agtcacggaa ccagacgcgc caggagtcca gcacctcgct gccccaggag
    24241 ttggagccga ggccaagtag ctggtggtcg aggttcaggg tgatgtcatc actgcgctgc
    24301 agctcgttac agtgctgggc agcgtggatg ttttcctggg tatagtgcca ggcgctgaag
    24361 ttaattgggc gctgcggaac caccagcaga ccgttgccgt ggcggttagt cagtgccgtc
    24421 cagcggacat gctgacggtt gccgttgttc tgcgggaagg gatagttctc gaacatggca
    24481 tcgacggtgc tgcgccagat atcgatgatg ttagcctgct ggctgtcggc gtagttttcg
    24541 cccggtccac gaccgtaata cgccacctga tcgtattcgc cgttaatccc catggtgaaa
    24601 ccgatgcacg gaatgatgtg cggatagtcg ccgtaacgct cgccggaaag cgccacgtta
    24661 acctggccat cggcagcgat gcgccagatg taggtacagc gcatcccgaa gtcaaacacc
    24721 ggcggtgcaa taactgtgcg gctgatgatc aacacttcac catcgctctg ttctacggca
    24781 aagtcgcgca gatgttcctg catgatctgc aaatgattcg gctgccacaa cccttcgtac
    24841 tcctgcttgt ggttgtcgat catcggcttg aagaagttga tctttggctc gcgggtcagc
    24901 agcgactcgc catttacctg ccaggatgtc ggtttgccac tcatttttga gaaggtgatc
    24961 gcgaagttgt agccgcgaac ggtgcagctc aaacgatcgt cttccagcgt cagcggacgc
    25021 gcattatttg gtgcgaaagg cactggctgc gcggtgtttt ccttcagcgg gaactgataa
    25081 gtggcgatag aatgcccggc ttcgctgtag cgggtgcggg aatctttggt caccgtaatg
    25141 ttgaggaacg cttcgcgggc gtccagctgc ggcagcgtga tctgcaaggg ggcttcgctg
    25201 ttcggcgcaa cgtcgcgcag tttaatctgc tgcgtcgcga gcgtttcacc ttcggcgcgc
    25261 acctctgcgt gcagggtgta gtcatcaagc gtggtaaacc acagtttatt ttcgactttc
    25321 aactcgccgc gagtcagcgc gtggattttt accggcgcga taacctgttt gtactctttc
    25381 aggcccgatc ccggcgtctg atcggaatag atcaaaccat caagacagaa gttatagttg
    25441 ttgggatagt cgccgtagtc gccgccgaat ttataccaga cattgccgtg gtcgtcctgt
    25501 gcctggatcc cgtggtcgca ccactcccag acataatgac cctgaatgca atcgtgctta
    25561 tagaagacgt tctggtactc cgtcagcccg cccggtccgt tccccatcgc atgagcatat
    25621 tcacagatga tgcgcggctt cggatgcggg tattcaccaa actcattcat cagcggcacg
    25681 cgggtgtaca tggtggaaat aatatcgacc acttcagcat cgcgatcttc ttcgtaatga
    25741 accagtcgcg tgtcatccag cgctttcgcc gcatggtaca tcgcgcggat gttacagcca
    25801 tagccggatt cattgcccag cgaccagatg atgatcgacg gatggttttt ctgtgcgtgg
    25861 atatggcgaa caatgcgctc gacgtagact ttttcccact gcggatcgtc ggtaatacgg
    25921 ctgatatcgc cgacattagc aaagccgtgc gattcgacgt cggtttccgc catcacaaac
    25981 aggccgtaga tatcacacag ttcgtaaaaa cgcggatcgt tcgggtagtg agcggtacgc
    26041 acggagttga tattgtgctg cttcatcaac tggagatctt tctcgacgcg atccattcca
    26101 acggcgcggc ctttgcgatg atcgttgtcg tgacggttga cgccgtgcag catcacataa
    26161 cggttattga tccagaacag accgtcgcgc actttgatat cacggaagcc aacgcgttgt
    26221 ggcaccactt ccagaacgtt gccgttggcg tctttcagcg tcatgaccag atggtaaaga
    26281 taaggggatt ctgctgacca ttgctgcggc tgttcgacag taaaagcaaa gctggcgctg
    26341 gtcagttttt caattgccaa atgatcaatg gcgctgctgt gcaccacgcg ttcgccatca
    26401 aacagggtat attccagcgt cgtgacgaca ggggaggcgg cgagattttc cagcaccact
    26461 tcgcaggaaa gcgtggcatc gcaataggct tcgtcaaagt cggtacgcac agtgaaatcg
    26521 ttaatatgcg ttaggtgttt tccgaccaga taaacatcgc ggaagatccc cgccgaccac
    26581 cacatatcct ggtcttccac gtaggtagag tccgcccact gcatcacgcg cacacacaac
    26641 aggttgtcgc cggttttaac catcgcgctg atgtcaaact ctgcggtcag gcgactgccc
    26701 ttgctgaaac ccacatactg accgttaacg tagacttcaa aataggtttc gacgccgtca
    26761 aatttaatca gcgtctgttt accctgccag ccgtcgctga gggtgaaaat acgttgatag
    26821 gcaccggttg ggttatcgct ggggacaaac ggcacatcga tggggaacgg aaaaccttcg
    26881 tcggtatatt gcagtttgcc gtgaccttcc atttgccaca tggcggggac ggtaatatgc
    26941 ccccagtcag ccattaactc agaggtgaag gcttctggta cttgcagcgg atggtcaaaa
    27001 aagtggaaat tccactgact gcttaagggc agaaacaggc tgctggtttc gcgggcaaag
    27061 gtacgcgctt gcgcaacaga atcatatgaa aaaaagtacg cacgcggcgc aagtcggttt
    27121 tcgtgggtga gctgaatgtt ttcccagcga ttcatagggc ctcccggtag agagaaatcg
    27181 gaaccattca tggtgatggt agtttgtgta attgctgggg gtttagtaaa atttttacta
    27241 aaaagcagca ggtaaatgca tttactttaa ctggatcacg aaaataaacg gcagcactgt
    27301 ccggcaaaaa tgattgccga acagtgtgta aggggattta acgggtcgtg ccgcgcagtt
    27361 ttaatttgct gggaacgaag actagcagcg gcagcgcgcg accatcgcgg gctttttcat
    27421 acaccaggtt aacgccctga cttcccatca tctcggaatg gatgcgcacg gtggagagcg
    27481 gcggaaaggt aaatcgcgcg gtggggatat cgttaacgct gataagcgaa atatcttgtg
    27541 ggatgttcag gccacgttca tgaattgccc gcagtacgcc gatagcaatg gaatcggaag
    27601 caacaaacag tgccttcgga tagtcttccc gcgccagcat ttgttttgcc agttcataac
    27661 ccgacgaact ggaaaaaccg ccgcgccaga tatcctcttc gcgtaccact tgtttcagtc
    27721 ggccatattc cgcaaaggcg acctcacgaa tatccgcctt gccaggctca tcttcaccgc
    27781 caataaaacc aatacgatta acgccctggt tgatatagaa gtcgatgatt tctttactga
    27841 tgcgtgccag atcgatatcc accgcatcgt aaccgctgcc gggttcgtga aagtcgataa
    27901 aacagatatt gtcggtcaac gcgctggcag cggcgcgcag ggcgggcgtg ggtttgccga
    27961 caattaaaat accggtgacg tttttaatgt ctggtaatcc gttgtgttca taacagttgg
    28021 tcagttcgat cgccagcttt tcgcactgag tttcaatgcc gtggcggatc gccagatagt
    28081 aaggatcgtt gatctccagc tcctgctggt agctgtagat agccagaata tggtgttggt
    28141 tgactgcgcc tgtctgtagt ttacgggcgc tactggtctt gtactccagc ttttcggcga
    28201 tctcgagaat gcgatgtttc gtctcttctt tcacattcaa tgtcggatcg tcatttaaga
    28261 ccctggatac tgtcgccagg gatacgccag cttcgattgc gatgtctttt aatgttgcca
    28321 tgtttacctt ttttactttc cctgatgtgc gcccccattg tagaggaagt gcttatcggg
    28381 tcacctgttt tcagtaaata aagcgcgttt ctttgacgct gtaacggtaa cacagcggct
    28441 taggaatgtg atcacgcctg cattctccat ttttagccta caggattgag aaaaactttt
    28501 ctatagttta gtaaaatttt actcgaaggg aggaagcaat ggaaaccgtg gcttacgctg
    28561 attttgcacg tctggaaatg cgcgtcggaa agattgtgga agtgaaacgc catgaaaacg
    28621 ccgacaagct gtacatcgta caggttgatg tggggaaaaa acactgcaaa ccgtgaccag
    28681 cctggtgccg tactacagcg aagaggagct gatggggaaa acggtggtgg tcttatgcaa
    28741 tctgcaaaaa gcgaaaatgc gtggtgaaac gtcggaatgc atgctgttgt gtgcggaaac
    28801 cgatgacggc agtgaaagcg tgttgttaac gccggagcgg atgatgcctg cgggcgtacg
    28861 cgtcgtataa aaagatcgga tggcgacgtc gtatcgccat ccgatttgat attacgcttc
    28921 ttcgacactt actcgcatgg ccgccagcgc cttacgcgcc gctttgatca ccagttcaca
    28981 ctgttcaatg gtcagtgtca gtggcggttc aatgcggatc gttttggcgt tattgagcgt
    29041 tccggccacc agtacgcgct ggcggaacat ctcgctggca aagttatagc cgatttcgtt
    29101 atcaacaaac tcaatcgcca tcaacatccc tttaccacgc gcttcctgta ccagatcggg
    29161 atattcccgc gccagttgac ggaaaccgtc cagcaacata tcgccttttt gctcagcctg
    29221 agccggtaag ttctgctcca gcaacacatt gatggtcgcc agcgccgccg cacaggccag
    29281 cgggttgccg ccaaaggtgg tggtatgcag gaatgggttg tcgaacagaa ctgaaaacac
    29341 ctcttcagtg gcgatggtcg cgccaatcgg catcacgccg ccgccgaggg cttttgccag
    29401 acacagaatg tccggctgaa cgttctcatg ttcgcaggca aacatcttgc ccgtgcgccc
    29461 catgcccgtt tgtacttcat cgaggatcat cagtgcgccg aactcatcgc acagcttacg
    29521 cacggcggtg agatagcccg gcggcggcag aattacgcca ccttcgccct gaatcggttc
    29581 gaggatcacc gcagccacat catcaccggt ttttttgcac tcattaagag ccgtgcgcat
    29641 ggcttcgata ttgccaaacg gcacatgacg gaaccctggc agcagtggca taaacggttt
    29701 gcggaaggtc gatttcgccg tggccgacag cgcgccaagt gatttaccgt ggaacacgcc
    29761 gctggtggca ataaaagtaa atttgccgcg cggcgactga taagccttcg ccagcttcag
    29821 cgccgcttca acggactcgg tgccgctatt acagaagaag ctgtatttca gtttaccggg
    29881 cgttagcgca gcaagggttt tcgccagcat agcccgtaac ggatcgagta gctcctggct
    29941 gtgcagtggt tgtttcgcaa gttgattctg tacggcggaa accacaactg gattacggtg
    30001 ccccacgttg aaaattccaa aacctcccag gcagtcgata aactcctgtc cctgggtgtc
    30061 gacaagcgta tttaaacctc ccgcttgcca ctctacggct ccgtaatccc cgccggcggt
    30121 aacagatttg cgatactcta aaaaccccgg attgacatgc tctttgaagt attcaatcac
    30181 ctctcggtta agtgctttca tctcctcatg atccagcgtt cgcttctcaa tgagattcag
    30241 ggcgtgggcg ctgcacgcta aagccgatgc gctcgaaggt aacctgttca aaatatgctc
    30301 cggaggctcg cgtatcacat gataccgatt taagtattgc agggattgcg ccactccggc
    30361 tgcgatcgcg aaaatcggga taaacacaaa tgcggttatc atttgtgcaa tatttaatgc
    30421 tatccgttaa catttgttgt tattttgcac ggtttttgtc gggtatggat ttgatgattg
    30481 ctgcgcaaat gcacgttttc agggcaataa ttgcctgatt tttgggcaag ataattaacg
    30541 ttttcgtata gtaacagagt gaaagtattt taaatcaaag aattagattt gtgctttaat
    30601 cgcaaattgc gatctaaatc aaattaatcg gttaaagata accgcagcgg ggccgacata
    30661 aactctgaca agaagttaac aaccatataa cctgcacagg acgcgaacat gtcttctcat
    30721 ccgtatgtca cccagcaaaa taccccgctg gcggacgata ccactctgat gtccactacc
    30781 gatctgcaaa gctatatcac tcatgctaat gacacttttg tgcaggtgag cggctttacc
    30841 ttgcaagagt tacaagggca gccgcacaac atggtgcgtc acccggatat gccaaaagcg
    30901 gcgtttgcgg atatgtggtt caccctgaaa aaaggggagc cctggagcgg catcgtgaaa
    30961 aatcgccgca aaaatggcga ccattattgg gtgcgggcca atgcggtacc gatggtgcgc
    31021 gagggaaaaa tcagtggcta tatgtcgatt cgtacccggg cgacggatga agagattgcg
    31081 gcggtggagc cgctgtacaa agcgttgaac tccggacgta ccagtaagcg tattcataaa
    31141 ggcctggtgg tgcgtaaagg ctggctgggt aaactgcctt cattaccgct tcgctggcgg
    31201 acgcgtggag tgatgaccct gatgtttatc ttgctggcgg ccatgctttg gtttgttgct
    31261 gccccggtgg tgacgtattt cctctgtgtg ttagtggtat tgttggcaag cgcttgtttt
    31321 gaatggcaga ttgtgcgccc gatagaaaat gtcgcccgtc aggcactgaa ggtggcgacc
    31381 ggagagcgta atagtgttga gcacctgaat cgcagcgatg agctggggct gacattacgc
    31441 gcggtagggc agcttggcct gatgtgccgt tggctaatta acgatgtctc aagccaggtg
    31501 tccagtgtca gaaatggcag tgagacgctg gcgaaaggca ccgatgaact gaacgaacat
    31561 acccagcaga cagttgataa cgttcagcaa acggtggcga ccatgaacca aatggcggcg
    31621 tcggtgaaac agaactctgc cacggcgtcg gctgccgata aactttctat caccgccagt
    31681 aatgcggcag tgcagggtgg ggaggcgatg accacggtga tcaagacaat ggacgatatc
    31741 gccgacagta cctagcgcat tggcaccatt acttcgctga ttaacgatat tgcgtttcag
    31801 accaatattc tggccctgaa tgcggcggtg gaagcggcgc gtgccggcga acagggcaaa
    31861 ggttttgcag tggtggcagg ggaagtgcgt catttagcca gccgcagcgc taatgctgcc
    31921 aacgatattc gcaagctgat tgatgccagt gctgataagg tgcaatccgg ttcgcagcag
    31981 gtacacgccg ccggacggac gatggaagat attgtggcac aggtgaaaaa cgtcacccag
    32041 ttgatcgccc agattagcca ttcaacgctg gaacaggccg atgggctttc cagcctgacc
    32101 cgtgcagtgg atgagcttaa cctgatcacc cagaaaaatg ccgagctggt ggaagagagt
    32161 gcgcaggtgt cggcgatggt gaaacaccgc gccagccgac tggaagacgc ggtgacggtg
    32221 ctgcattaat cggtatgccg gatccggcgg ttggagcaca atgcctgatg cgatgctggc
    32281 gcatcttatc aggcctgcgg ggtgtgtagc gggtgtaggc cggataaggc gcgtcaagcg
    32341 tcgcatccgg caattgcacc gcgccactgg cggatgcggc gattaatcca gttgcgtaat
    32401 atcaaaagcg gcgcggtcga tcaccgcaat gatctttttt atttgtgcat cactgatatc
    32461 gctctggttg acgcgtaaat ccagcaccgc tttaaaatta tccagcgccc gcttcatttg
    32521 cgggttctgg cgcagcgccg cgccaacgca acgcgctttg atgcgttctt caatcatctc
    32581 cacttgttcg cggttttctt ccagccactg cgcgccttgt tcggtcagcg caatctgctt
    32641 cttaccacct tcctcttcgc ggatggtaat cagcgactgc tcctgcagaa aatccagcgt
    32701 cgggtagatg acgcccgggc ttggggtgta attcccctgg gttagattct caatcgcttt
    32761 aatcaattcg taaccgtggc tgtcatcgcg cgagagaata tccagaatca ccagacgcaa
    32821 ttcaccgtga ccaaagaaac gttgccgacg accgccgccg cgaccgtggc gaccaccgca
    32881 acattgaccg tgttcatgct ggtgaccgtg tccgcagtgc tcgtgttctg acttctcacc
    32941 tttgcagcag ccctcatggc gtggctggcc ttcatgttta caacaccctt cgtgatgatg
    33001 gctcatatca gcctcctttt gcttgattag atatatctaa tttatatctg atgtgagcat
    33061 tactgcaagt acaaaaatga tttttaaacg tggaagcacc tcacatctag gccggataag
    33121 acgttcacgc cgcatccggc agcattgcta tggcactgaa tgccttcaca tacttcagac
    33181 atatcagttc tacaaatcgc ttgcatttat catgattaac aatcattatc atttgcgaat
    33241 tttatttaga tatatctgat taccatcacg aaggcgataa caatgaataa caccccccgc
    33301 tacccgcagc gcgttcgcaa tgatctgcgc ttccgtgaac tgactgtgct acgcgttgag
    33361 cgcatcagcg ccggttttca gcgcattgtc cttggcggcg aggcgttgga cggttttaca
    33421 tcgcgcggct ttgacgatca cagcaaactc ttctttcctc aacctgacgc tcactttgtg
    33481 ccgccaacgg taacggaaga gagcatcgtc tggccggaag gaccacgccc accgtcgcgt
    33541 gactatacgc cactgtatga cgaactacgc catgaactgg cgatcgattt ctttattcac
    33601 gacggtgggg tcgccagcgg ctgggcgata aacttacggt ggcaggtccg cgcggttcgc
    33661 tggtggtgcc ggaagattac gcgtatcagc tgtatgtctg cgatgaatcc ggaatgcccg
    33721 cactgcgccg ccgcctggaa acgttgagca aacttgccgt taaaccgcaa gttagtgcgc
    33781 tggttagcgt gcgggataac gcctgtcagg attatctcgc gcaccttgat ggttttaata
    33841 tcgaatggct ggcacatgat gagcaggcgg tagatgcgcg tctggcgcag atgcaaatcc
    33901 ctgccgatga ttacttcatc tggataaccg gcgaaggtaa agtcgttaag aatttaagcc
    33961 gccgctttga agcggaacag tatgacccac agcgggtacg tgcagcggct tactggcacg
    34021 caaagtaact gatatataaa aagaaaaagg ctgacgattt ctcgtcagcc tttgctatat
    34081 ctggtggccc ctgctggact tgaaccagcg accaagcgat tatgagtcgc ctgctctaac
    34141 cactgagcta aggggccatg gtagcggatt ataaagtaac tccgtgccgc aatccagcca
    34201 ttaccgcgcg cctgctgttt ttataaacaa tgtattttca atccgttata cttttcctgg
    34261 tgatgttaga aaggagtaaa tatggttgag gatattttgg ctccagggtt acgggtcgtg
    34321 ttttgcggta tcaaccctgg gctttcatcc gccgggactg gttttccctt tgctcatccg
    34381 gcaaatcgct tctggaaggt gatatatcag gccgggttta ccgaccgtca gttgaagccg
    34441 caggaggcac agcatctgct ggattatcgt tgtggcatca ccaaactggt agaccgtcca
    34501 acggtgcaag ccaatgaagt ttcaaagcag gagctgcacg caggtgggcg taagctgatt
    34561 gaaaaaattg aagattatca gccgcaggcg ttggctattc tgggcaaaca agcatatgaa
    34621 cagggattca gctagcgcgg tgcacagtgg gggaaacaaa cgctcaccat tggttcgacg
    34681 cagatttggg tgctgccaaa tcccagcggt ttaagtcgcg tttcactgga gaaactggtt
    34741 gaagcgtatc gcgagctgga ccaggcgctg gtagtgcgtg ggcgataa
//
LOCUS       DAJTSI010000027        34485 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000027 DAJTSI010000000
VERSION     DAJTSI010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.027"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(124..1632)
                     /locus_tag="OO327_001183"
     CDS             complement(124..1632)
                     /locus_tag="OO327_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR6017799.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(1733..2908)
                     /gene="manA"
                     /locus_tag="OO327_001184"
     CDS             complement(1733..2908)
                     /gene="manA"
                     /locus_tag="OO327_001184"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR6017800.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            3107..4753
                     /gene="fumB"
                     /locus_tag="OO327_001185"
     CDS             3107..4753
                     /gene="fumB"
                     /locus_tag="OO327_001185"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6017801.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            4896..6299
                     /gene="fumC"
                     /locus_tag="OO327_001186"
     CDS             4896..6299
                     /gene="fumC"
                     /locus_tag="OO327_001186"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR6017802.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(6296..7225)
                     /gene="tus"
                     /locus_tag="OO327_001187"
     CDS             complement(6296..7225)
                     /gene="tus"
                     /locus_tag="OO327_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR6017803.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(7301..8602)
                     /gene="rstB"
                     /locus_tag="OO327_001188"
     CDS             complement(7301..8602)
                     /gene="rstB"
                     /locus_tag="OO327_001188"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR6017804.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(8781..10100)
                     /locus_tag="OO327_001189"
     CDS             complement(8781..10100)
                     /locus_tag="OO327_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCR6017805.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(10325..11068)
                     /gene="rstA"
                     /locus_tag="OO327_001190"
     CDS             complement(10325..11068)
                     /gene="rstA"
                     /locus_tag="OO327_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR6017806.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            11197..11532
                     /locus_tag="OO327_001191"
     CDS             11197..11532
                     /locus_tag="OO327_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR6017807.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(11529..12251)
                     /gene="folM"
                     /locus_tag="OO327_001192"
     CDS             complement(11529..12251)
                     /gene="folM"
                     /locus_tag="OO327_001192"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR6017808.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(12288..13670)
                     /locus_tag="OO327_001193"
     CDS             complement(12288..13670)
                     /locus_tag="OO327_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6017809.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(13856..14800)
                     /locus_tag="OO327_001194"
     CDS             complement(13856..14800)
                     /locus_tag="OO327_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6017810.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            15324..16856
                     /gene="pntA"
                     /locus_tag="OO327_001195"
     CDS             15324..16856
                     /gene="pntA"
                     /locus_tag="OO327_001195"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR6017811.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            16867..18255
                     /gene="pntB"
                     /locus_tag="OO327_001196"
     CDS             16867..18255
                     /gene="pntB"
                     /locus_tag="OO327_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR6017812.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(18280..19314)
                     /gene="tqsA"
                     /locus_tag="OO327_001197"
     CDS             complement(18280..19314)
                     /gene="tqsA"
                     /locus_tag="OO327_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR6017813.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            19725..20090
                     /gene="mdtJ"
                     /locus_tag="OO327_001198"
     CDS             19725..20090
                     /gene="mdtJ"
                     /locus_tag="OO327_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR6017814.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            20077..20406
                     /gene="mdtI"
                     /locus_tag="OO327_001199"
     CDS             20077..20406
                     /gene="mdtI"
                     /locus_tag="OO327_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR6017815.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(20445..21266)
                     /locus_tag="OO327_001200"
     CDS             complement(20445..21266)
                     /locus_tag="OO327_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR6017816.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(21366..21449)
                     /locus_tag="OO327_001201"
     CDS             complement(21366..21449)
                     /locus_tag="OO327_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6017817.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(21542..21877)
                     /locus_tag="OO327_001202"
     CDS             complement(21542..21877)
                     /locus_tag="OO327_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR6017818.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(22274..23527)
                     /locus_tag="OO327_001203"
                     /pseudo
     CDS             complement(22274..23527)
                     /locus_tag="OO327_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            23634..24545
                     /locus_tag="OO327_001204"
     CDS             23634..24545
                     /locus_tag="OO327_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6017819.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAELAKEPFVFFDPHVGTGLYDDILGL
                     MRRYHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSE
                     MWLVWPKHHEQSPAARNFRIHLLNALR"
     gene            24680..25900
                     /gene="mlc"
                     /locus_tag="OO327_001205"
                     /gene_synonym="dgsA"
     CDS             24680..25900
                     /gene="mlc"
                     /locus_tag="OO327_001205"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR6017820.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            26025..26720
                     /gene="bioD"
                     /locus_tag="OO327_001206"
     CDS             26025..26720
                     /gene="bioD"
                     /locus_tag="OO327_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6017821.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(26673..27965)
                     /gene="clcB"
                     /locus_tag="OO327_001207"
     CDS             complement(26673..27965)
                     /gene="clcB"
                     /locus_tag="OO327_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR6017822.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(28124..28738)
                     /gene="dmsD"
                     /locus_tag="OO327_001208"
     CDS             complement(28124..28738)
                     /gene="dmsD"
                     /locus_tag="OO327_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR6017823.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(28781..29635)
                     /locus_tag="OO327_001209"
     CDS             complement(28781..29635)
                     /locus_tag="OO327_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR6017824.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(29637..30254)
                     /gene="dmsB"
                     /locus_tag="OO327_001210"
     CDS             complement(29637..30254)
                     /gene="dmsB"
                     /locus_tag="OO327_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR6017825.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(30265..32303)
                     /locus_tag="OO327_001211"
                     /pseudo
     CDS             complement(30265..32303)
                     /locus_tag="OO327_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(32364..33473)
                     /locus_tag="OO327_001212"
     CDS             complement(32364..33473)
                     /locus_tag="OO327_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR6017826.1"
                     /translation="MINVSHIRTLSSIESTTSSPSIRLAMESIWNYAGNTLVNQHSDI
                     NKTHEILQDESKCEMIVVIENFMTSSAKYADILLEDLMTVEQEDIIPNDYAGNMGYLI
                     FLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPAL
                     PSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWE
                     LEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQE
                     VWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEPNMSGD
                     KIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
     gene            33456..>34485
                     /locus_tag="OO327_001213"
     CDS             33456..>34485
                     /locus_tag="OO327_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6017827.1"
                     /translation="MTDINHDNTVYPSSSFSGSPYRYQQVYTSSPDTRLTQINLLSVA
                     KPQLVRRANGRYTIRFRLKGQTTPFLSVSTRSTDRRVATMRQRELAATAKAFMLDRPE
                     VSLQELTEHLRSMAEQFLTDASDDYWNGLEVATLVDEKSNLKELAATQALSLDQQKGI
                     RLALEVLTAAQQRVDTGDTSGLIKLIDDNNLTDDSTIGDSTSILNNEQGDRPAVFTQE
                     RQSSVVFSSLVSSLLAEKVQTLKTSSYKDLSSSLNTVSRFLPEDMDLMSRSGWLAVRD
                     SMLASEVRPSTINKLLTKAKMCLDYGLMNGQLEGRNPIERMKLTKDIDSKRRAFTDEE
                     LERLLVRVE"
BASE COUNT         8788 a         8465 c         8782 g         8450 t
ORIGIN      
        1 actcataaat tcaaagtgtt gcaggaaaag gtaggcctga taagcgtagc gcatcaggca
       61 atctctggtt tgttttcaga tgaaaacgcc ggagtgaaaa ttctccggcg ttttggccgt
      121 gaattactgc tgcggaattg ccggtacagc cggaacgtta agagctggca tcgcaaacat
      181 gccaacaaaa tcttctaacg acattttctg cccatttaac gttatctgac cgttagcata
      241 ttgcaggctg gtggtgatgg tattgtcctg caaggtggtc agacggaaca tctgccccat
      301 tgctgatgca ccttcaactt gctgtttcgc cagttttttc gcttgatctt cctgataacc
      361 ttccagcttc gctacctgag tcatcaactc agttgccata tccaccggaa tggtcagttt
      421 cgcatccaga gatttaaccg aacgatctac ttcctgcgcc agcgtttgcg gcgcttcttt
      481 agtcgttgcc ggatctttca ggaacagcga cagattcagc gcactttcac cctgactgtt
      541 tttccagctt agcggcgcga tagtaatcac cggatcgcct ttcaacatca gcggcagggc
      601 gctaaagaag gcttccgtca ctttctcctg ataaagttcg ggattgttgg caatttctgg
      661 ctgtgccagc agcgcctgag tttgcgcgtt atattgctgg ctaaactgat gccaggcttc
      721 accatcaatc tggccgactt ttaaagtcag cttgccgctg cccagatcct gattctgtac
      781 cttcaggctg tttagcgagt aatccagttg gctattgatc gttttaccgt cattgaccag
      841 atccgattta ccgctgatct ccatgccttc cagcagtgcc agttctttgc cttccactga
      901 aatggtcatt ttttccagtg acagtttttg gtttcctacg cgctcaccaa aacttcccag
      961 cgtgctggaa ccgtcggttt tcagattatt aaaggtcaac tgcactttct ggttgtattc
     1021 gttaactgcg tctatccgac cactttgcgc ctctccggaa agggagatgg ctttgccgtc
     1081 tctgtcagca tttaactgga actcgccgcc gctaaaggcg actttttcat ccttttgctc
     1141 gtaattcagt ggattgagcg aaatatcgga actggaatca ccgctgtaac caatgcgcga
     1201 gttaatctca aaaggcgttt caccttttgc catatcaaac agtggtttgc ttacttcgtt
     1261 attaaccagc gtggtttgaa ttgatgccat cgacgggatc aggttcagtt ttttaagctg
     1321 ggcaagcggg aagggaccat gatcaaccga ttcgttgaag atgacgctct gaccgctttt
     1381 aatccacgga ttttctttcc cggcaatggg tttcaccaac agttgcaact ggctgctgaa
     1441 tacgccgcga tgatagtttt gataactcac ttccaggttg gattcaggag ctgtcagttt
     1501 gagttgcgcg ttcgcctgcg cgaccatgtc ttcgagatgg gtttcaatct tcttgcctgt
     1561 ataccatgcg ccgcctgtcc agactacgcc tagcgcaaca atgacgccta ccgctaccag
     1621 cgatttattc ataatgatta tccataaaat gaaatcaggc ggactggccg cctgaaggtg
     1681 ttataagcct ttaataagct tagcaagaga tgttaatttt ttcagtaagc tcttacagct
     1741 tgttgtaaac acgcgctaaa cggccgtggc ctttgacagt caccggtgat tcgttggcgg
     1801 caataaacgc tgattcaccc ggtttaagct gtaactgctg agaacctttc cacaacgttg
     1861 catcgccttc gacgcagaac aaaatggcgg cactctgctg gctgatggtg gtttctttat
     1921 cactaaggtc atgcagcgag aaggcaaaat catccacagg aatcgggaag tccagttctg
     1981 caccttgttt caccggctgg gtcaacaact ggttagccgg tttggcttcg aatttcacat
     2041 tggcaaccag ttccggaata tcaatgtatt taggcgtcag acctgcacgc agcacgttat
     2101 cggagttcgc catcacttcc agcgccacgc cttgcaggta agcgtgcggt gtttcagcga
     2161 acaggaacat cgcttcgcca ggattcaatt tcaccacatt cagtaacagc ggggagaaca
     2221 ggccgctgtc ttccgggtaa aattcagaaa ttaaacgaat cgtttgccac ggttcaccct
     2281 gctgactgtc gagcgccgat tttaaaatcg ccagcgcgcg ggatttttct tcaccctgca
     2341 tattcaacag gttggcgaac agttcgctta aacgttcggc atgtggctgt tgtaaaaagt
     2401 gagcaatcgc cggatgtgca ccggcgaccg gctggagtag ggagacaatc tcggaaaatt
     2461 cacgaaacgc gttcatcgca aggaaaggcg tcagcgcaaa aaccagctcc ggcttgtggt
     2521 taggatcttt atagttacgc tcggcggcat ccatcgggat acctgcggca ttttctttgg
     2581 caaaaccgat ttcagaattg tgtttgtttg gatgaacctg aatggagagt ggctgtgctg
     2641 cgcataatac tttgaacagg aaaggcagtt cgccaaagcg tttggcaacg gcctctccga
     2701 gcagagtcga tttatcactc tcaatcacat cacgcagtga aacgatatct ccggcggcat
     2761 tctgcactcg tgaactgctt ttcggatgtg cgcccatcca cagctcggcc atcggctggc
     2821 tggacggatt ttccatacca taaagttcag tcaacgccgt tttgctaccc caggcatagt
     2881 tttgcactga gttaatgagt ttttgcatga tcaatccctg ttttaatgtg gaaattaatc
     2941 ccgctattaa agcaagaatc ctacgggaag taacctggag ccgcaaaaag tcgtactagt
     3001 ctcagttttt gttaaaaaac tgtgtaggat attgttactc gcttttaaca gggcaacgga
     3061 acacccgccc agagcataac caaaccaggc agtaagtgag agaacaatgt caaacaaacc
     3121 ctttcattat cagtctcctt ttccactcaa aaaaaatgat actgagtatt acctgctaac
     3181 cagtgaacac gttagcgtat ctgaatttga agggcaggag attttgaaag tcgcacccga
     3241 agcgttaact ctgttggcgc gtcaggcgtt tcatgatgcg tcgtttatgc tgcgtccggc
     3301 tcaccaacaa caggtggccg acattctgcg tgacccggag gccagcgaaa atgataaata
     3361 tgtggcgctg caattcctgc gtaactccga catcgcggcg aaaggcgttc tgccaacctg
     3421 tcaggatacc ggcaccgcga ttattgttgg taaaaaaggg cagcgtgtat ggaccggtgg
     3481 tggtgatgaa gcggcgctgg cgcgcggtgt ctataacact tatatcgaag ataatctgcg
     3541 ctactcgcaa aacgcgccgc tggatatgta taaagaagtg aataccggca ccaatctgcc
     3601 agcgcagatc gatctttatg ccgttgatgg cgacgaatac aaattcctct gtatcgcaaa
     3661 aggtggtggt tcggcaaaca agacgtatct ctatcaggaa accaaagcgt tactgacgcc
     3721 ggggaaactg aaaaattacc tggttgagaa gatgcgcacg ctgggtaccg cggcctgtcc
     3781 tccgtatcat attgcgttcg ttattggtgg aacttctgca gaaacgaacc ttaaaacggt
     3841 gaaactggct tccgcgaaat actatgatga actgccaacg gaagggaatg agcacggtca
     3901 ggcgttccgc gatgtggaac tggaaaaaga attactgatc gaagcgcaaa atctcggtct
     3961 gggtgcgcag tttggtggta aatacttcgc tcacgacatc cgcgtgattc gcctgccacg
     4021 tcacggcgca tcctgtccgg ttggtatggg tgtttcctgc tctgctgacc gtaatatcaa
     4081 agcgaagatc aaccgtcagg ggatctggat cgaaaaactg gaacataatc caggtaaata
     4141 tatccctgaa gagctgcgta aagcgggaga aggcgaagcg gtgcgcgttg accttaaccg
     4201 tccgatgaaa gagatcctcg cacagttgtc gcagtatccc gtttctacac gcttatcgct
     4261 taacggcacg attatcgttg gtcgtgatat tgctcacgcc aaactgaaag agcggatgga
     4321 taacggtgaa gggctgccgc aatacattaa agatcatccg atttactacg cgggtccggc
     4381 caaaacgccg gaaggttatg cctccggttc tcttggccca acgaccgccg gacggatgga
     4441 ttcttatgtc gatcaactgc aagcgcaggg cggaagtatg atcatgctgg cgaaaggcaa
     4501 ccgcagccag caggtgacgg atgcctgtaa aaaacacggc ggcttctacc ttggcagtat
     4561 cggtggtccg gccgctgtat tggcgcaggg aagtattaag agcctggaat gtgttgaata
     4621 tccggaactg ggaatggaag ccatctggaa aattgaagtg gaagatttcc cggcgtttat
     4681 ccttgtggat gataaaggaa atgacttctt ccagcagata caactcacac agtgcactcg
     4741 ctgtgtgaaa taaacagagc cgcccttcgg ggcggttttt ttacatggca cgaaagacca
     4801 aacatttgtt atcaaatagt aaataatgag tgagctaaaa gttgcttaac gaaagcaaaa
     4861 cagaaagaaa aaattaatca ggtgaggagc aggccatgaa tacagtacgc agcgaaaaag
     4921 attcgatggg ggcgattgat gtcccggcag ataagctgtg gggcgcacaa actcaacgct
     4981 cgctggagca tttccgcatt tcgacggaga aaatgcccac ctcactgatt catgcgctgg
     5041 cgctaaccaa gcgcgcagcg gcaaaagtta atgaagattt agacttgttg tctgaagaga
     5101 aagcgagcgc cattcggcag gcggcggatg aagtactggc aggacagcat gacgacgaat
     5161 tcccgctggc tatctggcag accggctccg gcacgcaaag taacatgaac atgaacgaag
     5221 tgctggctaa ccgggccagt gaattactcg gcggtgtgcg cgggatggaa cgtaaagttc
     5281 accctaacga cgacgtgaac aaaagccaaa gttccaacga tgtctttccg acggcgatgc
     5341 acgttgcggc gctgctggca ctgcgcaagc aactcattcc gcagcttaaa accctgacac
     5401 agacgctgag tgaaaaatcg cgtgcatttg ccgatatcgt caaaatcggt cgaacccact
     5461 tgcaggacgc cacgccgcta acactagggc aggagatttc cggctgggta gcgatgctcg
     5521 agcataatct caaacatatc gaatacagcc tgcctcacgt agcggaactg gctcttggcg
     5581 gtacagcggt gggtactgga ctaaataccc atccggagta tgcgcgtcgc gtagcagatg
     5641 aactggcagt cattacctgt gcaccgtttg ttaccgcgcc gaacaaattt gaagcgcttg
     5701 cgacctgtga tgccctggtt caggcgcacg gcgcgttgaa agggttggct gcgtcactga
     5761 tgaaaatcgc caatgatgtc cgctggctgg cctctggccc gcgctgcgga attggtgaaa
     5821 tctcaatccc ggaaaatgag ccgggcagct caatcatgcc ggggaaagtg aacccaaccc
     5881 agtgtgaagc attaaccatg ctctgctgtc aggtaatggg gaacgacgtg gcgatcaaca
     5941 tgggtggcgc ttccggtaac tttgaactga acgtcttccg tccgatggtg atccataatt
     6001 tcctgcaatc ggtgcgcttg ctggcagatg gcatggaaag ttttaacaaa cactgcgcag
     6061 tgggtattga accgaatcgt gagcgaatca atcaattact caatgaatcg ctgatgctgg
     6121 tgactgcgct taacacccac attggttatg acaaagccgc ggagatcgcc aaaaaagcgc
     6181 ataaagaagg gctgacctta aaagctgcgg cccttgcgct ggggtatctt agcgaagccg
     6241 agtttgacag ctgggtacgg ccagaacaga tggtcggcag tatgaaagcc gggcgttaat
     6301 ctgcaacata caggtgcagc cgtggaatga tcaaacgaag cggctgcgcc tgaggtttat
     6361 aacggtgctg cacattgtcg gcatcgtaat ttaacaactc accaacgtcc ggcacgcccg
     6421 cgccattatc ccgattaatc agtgcaatca gtggtgtagg gcaggcgtgt tggacttgtt
     6481 tttgatctcc tttgtaccag acgcgggcaa tcggctgcac cttcactgga cgtttgattt
     6541 ttaacttcgc gttctgtggc agggcagcga tatcctgata ctctcgctcc agttttcttt
     6601 gccactcctc gcgcgtccac ggtgcgacac tgcgtggtga tttcaggctt ttttccagct
     6661 gtgccaggac ttcatcacga tgcaaattct taatgatatg tttattagcc caaccaaagc
     6721 gtaaagtggc ggggtcgtgc agaacggtga gcgtgcggta agcattaagg gtgatcagcc
     6781 ccggcaaatg acgatgcacc cattcaaaac gtgccgcggt ggggagttct gattcaaccg
     6841 tgacgatatg ctcgaacgtg gtcttgagtt tattgatgtg ctgaatatga ctgaccaacg
     6901 ctgcttgcga aaggttatcg acctggtaac acaacacgcc aggcagacgg acagcggcct
     6961 tgctgctgcg attttcggac tgttgttgaa taaataaatg gcggaaatga cgcaacgcca
     7021 gcgactgcgc gtcgttgccg agatgttgtt ttacctcaat acgattaagc ggattatgct
     7081 catcctcttt ttttacctcc ggcaaagaga acacgcgggc aaccaatagc ttgtgttgct
     7141 caagatgagc ggcaaatgta gccagctctt gttccatctg gcgaaaggta gtgttgagtc
     7201 ggtctacgag atcgtaacgc gccataatat taaccacttt agttacaaca tacttatttt
     7261 atagcacagt cgtggtgact tgaccatcgt gcgcgtagtg tcaggcagag gtaaattgcg
     7321 ggatgttatg ccataacggc cagctaaacg agaagcgagc accacccagt tcgctgatgt
     7381 cacaattaac cgtaccgccc attgtcagtg ctattgagtg gacaattgcc agccccagcc
     7441 cgcagccgcc ggttgagcga tcccggctgg gatcgaggcg aacaaaaggt tcaaagatat
     7501 gttcgcggtt ttctggggca atccctgggc catcatcctc aacaattaat gtcgctctat
     7561 tccccgacag tagcaggctg gtttcaaccg ttgaatggca gtagcgcagg gcgttattga
     7621 gcaaattatc cagcacgcgc tccattaagc gcatatccaa cgccgcataa tggccttgca
     7681 tgagcgtttt tatccgtacc gttttatcgg gcgttactgc ctgaatatct gccagatgcg
     7741 ttgacagcca caacggcagg tctggttcgc taagatgaag ctcgttttgt gggcgatcga
     7801 gtcgggcata agtcagcagc tcttcaatta aagcttcaag ttgactgata tcacgattca
     7861 acgcctggga ttcggcggcg ctcaggttat cgctcatctc cagtcgataa cgcaggcgca
     7921 ctaacggtgt tcgcagttcg tgagcgatac cgtcaataag ctgttttttg ctggcaatta
     7981 aggcgttgat attgtccgcc atctggttaa atgcgatacc aagtcgttca aagctcgaac
     8041 cctcatcaaa gtggatacgt tcattgagat gcccatcgcc aaatcgttgc gccgctgctt
     8101 ccagttttaa catatcctgc cagtgcggac gcatccagat aaacaccgga aaggcgaggg
     8161 aaatagcaat aaaagcgatc agggcgatat ccagcaatcg catttgatgg aggtaataaa
     8221 gataaggaac aggaccaact gccagcacgt agtggctgcg cgggatacgc tgcaaaaacg
     8281 tgtactgatc gtccagggcg acaatttcgc cgccacgcag tcggtgcatg gaaatatcat
     8341 caagatggta tttactcagt ggctcgacac gcagatcgaa agagagattt aaatccatct
     8401 ctttcagcgt cttaccccag tcgtgtgggg ggatctctcg caattcgctg cgcatcagat
     8461 acagcgaact gttcatcaaa tcatccagcg actgtttgcc cgcgcgttcg gcggtaaatt
     8521 tgtacaccag cccaaccagc agagacatca caaggaagca gacaaacaat aacaggtaaa
     8581 actggataaa caattttttc attggcatcg cccgcaacag aatactattc gtattaattc
     8641 acgactgtgg agcaatgtaa accgccaact ggataagacc ttgtttggaa gataatgtcc
     8701 ggaacagacg gatgtatccc cggtgtattc atattcagta aattcaattt tccaccgggg
     8761 cttgtttatt atgctaaggt ttaatttaac gtgacgcttt gttgttcttt agtctctcta
     8821 tccttaagaa gacgggctga tattacaccg gacattattg aaccattcac attaagcgct
     8881 gttcttgcca tatcaacgat cggctctatg gcaataaaaa taactactaa tgcgacaggg
     8941 aaattaagtg ttgaaaggac aatcagcgcc gcgaatgtcc caccactacc tacgcctgct
     9001 acccctattg agccaagcgc gatggcaggt aaaagagttg cgaggaattg caatgagaag
     9061 ggatcgatac ccattgttgg agcaatcatc gccaccatca cggcaggata aatcccagcg
     9121 cagccatttt gtcccatgct ggaaccaaaa gacgctgcaa tattagcaat cgtatttgga
     9181 acccccaatt tttccaatgc agaaatagcc agaggaattg aagccgcact actgcgagaa
     9241 acaaacgcaa aggtaagtac ttgccagacg gtcttgtaat agttaacggg gtttttacct
     9301 attaacagca aaatgcccga gtgaacaata aacatcacaa aaacggccag ataacaggcg
     9361 acgatgaacc cgagcaaatt ggtaaactgt gaaagctgat acgttgagaa gactttcgcc
     9421 attagtgcca ttaccccata aggcgtcagc gcaatgacaa tacggaccat tttcattacc
     9481 catatttgaa tcgcgttgat ccccgcgctc aatttttcgc cctctgctgg tgcatctttc
     9541 tttactttta acagcgcaat accagcaaga attgtaaaga tgacgatgcc aataaccgaa
     9601 acacttcttg ccccggtcag atcaaggaag aggttggttg ggatcaatga cgtaatgagt
     9661 cgcggtaaag ataccgcagc tgttttcgag acgtcatcgc tggttaaaga ggattgcata
     9721 tgaccaaagg cgctggcatc aaggccaatt gcactggcaa tagccaggcc aactaaacct
     9781 gcaatcatga ctaaacacaa catacaggca acaatagtta aagacatctt gccaatacca
     9841 gcgctatttt cgagcttatt aatcgctgaa agaatcgaaa tgaatatgag tggaacggca
     9901 acaagtttta ataaattaac ataaccgtta ccagcaatat tataccattc gataacggtt
     9961 tcactgcttg tctcaggaat gaaatgcagg gagataccaa tgatagcccc caggaaaatc
    10021 gcgataaaaa cacttttgga aagggattcg ctcttttgat ccgttttgaa aatcccccat
    10081 gagcagatca aaaaaagcac aatatatgtg aaagttgtaa ccgtttttta agcctcttgc
    10141 ctcattcagc acctgaaatg ccggaaagag tttgttgtaa aaaatctgcg ccataagacg
    10201 cagggacgag gcgattatag agaaattttt tctcattttc atatcaaatg tagggggttt
    10261 ggaatttgaa taagctctta ttaattgaat tcgttaattg ttctcttttt cagatgaaca
    10321 aatattagtc atctgattgt ttattgtcac tatcccatgc atgaggcgca aaaagatagc
    10381 ctttgttacg cacagtttta atgcgataag gttctgcggc gttatcgagc agtttttttc
    10441 ttaaccgcga aatagccacg tccacgctac gatccagtcc gtcataactg acgccgcgta
    10501 aatttttcag caatgcatcg cggtccatga tttgcccggc atgcgtagct agttcccaca
    10561 ataattcgaa atcagctgtc gagagcgaga tttcagtgtt agccagggtg actacgcggt
    10621 tgatggggtc gatagttaac gtgccgaaat gcagggcttt gtagggagta agagacgttt
    10681 cctgaagacc tttggtcagt gtggcttgct cattctgacg caaatgcaaa cgtaaacgcg
    10741 ctagcaaaac agcagggggc gtcgttttga gaatatagtc gcaggcaccc atttccagtg
    10801 ccaggatgtg gttcatatcg ctatcgagag aggttagaag aacaatcggt ccagaccact
    10861 ttgcgcgtaa atcacgacaa atggtcatgc cgtccttgcc tggtagcatg atgtcgagta
    10921 acaccaaatc cggattttct cgcaaaatgg tttcttcggc ctggtcgccg cgcggttcta
    10981 cggtaacctg catatcatgt tttgccaggt acgcggcaat cagtgaaccg acttccgcat
    11041 catcttccac aaatacgata gtgttcataa cattcacggt agatataaaa acgtcacaat
    11101 acaccgcgct gtttttactt actatcaatc gtttctaaac aagttttcat caggtattct
    11161 actgatgact tgttatggaa gtgttaaggt aaaaagatgg ggctggtaat caaagccgcg
    11221 ctcggtgcgc tggtagtgct gttgattggt gttttagcaa aaacgaaaaa ttattatatc
    11281 gccgggctga ttccactttt cccgaccttt gcgcttatcg cgcattatat tgttgccagc
    11341 gaacgcggca ttgaagcctt acgcgcaacc atcattttta gtatgtggtc gattattccc
    11401 tattttgttt acctggtgtc gctgtggtat ttcaccggaa tgatgcgctt gcccgccgct
    11461 tttgttggtt ctgttgcgtg ttgggggata agcgcatggg tgttgattat atgctggata
    11521 aagctgcatt aacgcagatg acgaccgcca tcaagtggga aactgcgtcc ggtgacaaag
    11581 caactggtaa gtaagtaatc gaccaggtcg atcacttctt tctcgccagg cgcggttttc
    11641 atcagtgatt tattcagcgc ctgttgtcga tattcggcat catcatgttc attaaacagg
    11701 atcagcgatg gcgcaataga attcactttc acttccggtg ccagcttgcg ggcaaacgag
    11761 cgggtcatat tatccagtgc cgctttgctt gcagcatacg caacatgttt gtcgctaccg
    11821 cgctccacca cataatcggt aaagtgaatg atatcgctgg cggcgtgtcc gtgcccacgc
    11881 agtaatcttt ccagcgcatg gttgagcagg tatggggtat taacgtggat ctgcatcatg
    11941 caagccagta cgtcggccag tggcgcaccc ggtttttccg caatccacgc actggcgtta
    12001 tgcaaaatag cacgcagacc atgggtggtt tttagtactt catcggcaaa cgccatcaca
    12061 ccgtcgttgg tcgaaaaatc agcctgaata cactgcgcac ctgcattaat cagtccatca
    12121 atggctggat agtgtgtccg atagctgaca atcaccggtt gcttttgatt aatgaaatgc
    12181 catgcgaggg cgaggccgat gcgacgacct ccgccagtaa ttaatattgg caagggctgg
    12241 gttttaccca tcgttatctc ctttgctatc caacgatggg atgaacgtta tcccaccagc
    12301 atccaggtcg ccggaactga ggcaattaac agcatgccaa tcagcaccat ttcctgacgg
    12361 ttcagtacat tatcatgtgt atgcgttttg cgcgcataca ggaaaaccag cagccccggt
    12421 gcatacagaa caacggataa cagcaagtgc attggaccag aggcgtacaa taaccataag
    12481 ccataaatgc aggcaccgac acctaccgct ttatgcagtg gacgggttgc gattttcagc
    12541 aagaacgcgc cgaccaggaa atagggcacc agaatcattt ctgaggcgat ggtcagcaac
    12601 gtgttgtaat cggaaccggt cagccagatg agtaccagac aaatttgcac gcagatgttg
    12661 gtcaaccaca gtgaggcgga tggcgcagct tgtgcgttct ggcgcgcaaa aatgcgtggg
    12721 aatgctttat gagtggcagc caggaacgga acttctgccg ccatgatggt ccagcttaag
    12781 tacgcgccgc aaacggaaac gatcaaaccg gcagcgatga tgatttcgcc ccatggtccc
    12841 atcatttcca ccatcagacc ggccattgac gggttacgaa tttcagccag ttcaggacgt
    12901 gccaccacac ccagcgaaag cagcgttacc agcaagtaaa cgcccagagc ggagagaacc
    12961 gccagcagtg tcgctttgcc aacatcacgt ttattacgcg cacgcgcaga aacaaccacg
    13021 gcaccttcca caccaatgaa aacccacagg gtgatcagca tggtgttttt cacctgttcc
    13081 caaacaggta cgccaagtgc aagtccggtg aagtcgagct tgaaggtatc cagtttgaac
    13141 atcatcatcg ccagcacaac aaacagaccc agcggcaaca attttgccag tgtcgccacc
    13201 aggttaatgc tggcagcggt ttgcaccccg cgcaggatca aaaagtgaac aatccacaat
    13261 aaagccgatg caccgactat cgattgccag gtattaccat cgccaaacag gcgcaattcc
    13321 ggcgtgtccg taaaaaagct taacgcggaa aaaacgatca ccagatagga gacgttggcg
    13381 atgaccgcgc acagccagta tccccatgcg gaacaaaagc cgattagctc gccaaaccct
    13441 tcgcgggcat aggtaaagat accgccatca agttcgggac gaatgcgcgt gaggatcagc
    13501 atggcaaagg ccagcaataa aatgccagcg ccagtaatac cccagccgat gagcagtgct
    13561 gccgggctgg caactgccgc catattttgc ggcagactga aaacacccgc gcccagcatt
    13621 gagcttaata ccagcgcggt gagtgcgctc agtcccagtt tcttttccat cgatatccag
    13681 tgattaaaac gtgataagaa gaataaatat tagggcaaaa cgcataaaaa tggagtttgc
    13741 cactgaagcg cgggatttta cgaagagatg tgtctgcatg caatggtcgc ggggaaattt
    13801 gtgactaaaa atgcgaaagg cggcatcaag ccgccttata ggagtaactg aatagttatt
    13861 tatacagatc tgcgctgacg gtcaggttat taccacgttt ctgccactgg cgggtgatgt
    13921 ggtaatactt agcacctttc ttcgcggcac gtttcgcaac ctgataggag acttcggtca
    13981 tgttgccgta gttgccagag aatttgatgc tgtcgaacgg gaccatcatc gctgctgtcg
    14041 ctttgttcag ttcttcgact ttagtgccat ccgggagcgt gacggtgtaa cgcccgcctt
    14101 ttgatgactg ggtttcaaag aagcgaccga cttcagaact tggtgatgcg gtagtcgcaa
    14161 cacccgggat ctcaactttc ttcgcggctt cgccaccggc agccagagct gcacgtcctg
    14221 cttcggaatc tgccgggatc acatccgggc tctggacgat acgtttctta gcatcttttt
    14281 tatagatgaa tgcagtaata cgctggttgc cgccctggtt ggcatcgatt tgacgaacga
    14341 tgtagaaaga gtaggcacct ttcgcttttg ccgctttggt gatggcatca ttgacttctg
    14401 gctggctacg atagaagccc tggacggtga ccgtgtcaaa cggttcaatc agaacagcct
    14461 gatctttcgg cagttcgaca acaccgttaa ttacgcgatt acttgtttct tctgcttttt
    14521 cagcatcggc tttatagagg tcagcgacca cacgccagtt accgctgtta ccaaaatcag
    14581 aagtgtcgac aacataaaaa gaggcggcac cttctttatc tgcgcgacga gaaacggctt
    14641 tcaccgcttc gccaatagca ttaaaacgac cggtaaccac tacacggtca aaaggtttaa
    14701 ccgctgccgc ttgctccggt gtcagttctg ttgctgcgtt aacggagaat gccgtagcag
    14761 aaagcagtgc cgacgccagg agggtgttct taagcttcat aaaaataatc cttcgccttg
    14821 cgcaaaccag gtactggtat tgttattaac gagaaacgtg gctgattatt gcatttaaac
    14881 ggtgtaactg tctgcgtcat ttttcatatc acattcctta aaccaatttt aatcctgctc
    14941 aaatgaccgt ctatgcttaa aaaacagccg tatcagcatc attactactg aagcaactga
    15001 attgtataag ttaatttaat gttaagtagt gattcgtgcc ggggcgatgt ctcgttttac
    15061 ccgaccgtcg aagacaatta tcagtcttta tccggcgttc taaggtgttt atcccactat
    15121 cacggctgaa tcgttaatat tttgcgagtt cacgccgaaa tactgatttt tggcgctaga
    15181 tcacaggcat aattttcagt acgttatagg gcgtttgtta ctaatttatt ttaacggagt
    15241 aacatttagc tcgtacatga gcagcttgtg tggctcctga cacaggcaaa ccatcatcaa
    15301 taaaaccgat ggaagggaat atcatgcgaa ttggcatacc aagagaacgg ttaaccaatg
    15361 aaacccgtgt tgcagcaacg ccaaaaacag tggaacagct gctgaaactg ggttttaccg
    15421 tcgcggtaga gagcggcgcg ggtcaactgg caagttttga cgataaagcg tttgtgcaag
    15481 cgggcgctga aattgtagaa gggaatagcg tctggcagtc agagatcatt ctgaaggtca
    15541 atgcgccgtt agatgatgaa attgcgttac tgaatcccgg gacaacgctg gtgagtttta
    15601 tctggcctgc gcagaatccg gaattaatgc aaaaacttgc ggaacgtaac gtgaccgtga
    15661 tggcgatgga ctctgtgccg cgtatctcac gcgcacaatc gctggacgca ctaagctcga
    15721 tggcgaatat cgccggttat cgcgccattg ttgaagcggc acatgaattt gggcgcttct
    15781 ttaccgggca aatcactgcg gccgggaaag tgccaccggc aaaagtgatg gtgattggtg
    15841 ccggtgttgc aggtttggcc gccattggcg cagcaaacag tctcggcgcg attgtgcgtg
    15901 cattcgacac ccgcccggaa gtgaaagaac aagttcaaag tatgggcgcg gaattcctcg
    15961 agctggattt taaagaggaa gcgggcagcg gcgatggcta tgccaaagtg atgtcggacg
    16021 cgttcatcaa agcggaaatg gaactctttg ccgcccaggc aaaagaggtc gatatcattg
    16081 tcaccaccgc gcttattcca ggcaaaccag cgccgaagct aattacccgt gaaatggttg
    16141 actccatgaa ggcgggcagt gtgattgtcg acctggcagc ccaaaacggt ggcaactgtg
    16201 aatacatcgt gccgggtgaa atcttcagta cggaaaatgg tgtcaaagtg attggttata
    16261 ccgatcttcc gggccgtctg ccgacgcaat cctcacagct ttacggtact aacctcgtta
    16321 atctgctgaa actgttgtgc aaagagaaag acggcaatat cactgttgat tttgatgatg
    16381 tggtgattcg cggcgtgacc gtgatccgtg cgggcgaaat tacctggccg gcaccgccga
    16441 ttcaggtatc agctcagccg caggcggcac aaaaagcggc accggaagtg aaaactgagg
    16501 aaaaatgtgc ctgctcaccg tggcgtaaat acgcgttgat ggcgctggca atcatcctgt
    16561 tcggttggct gtcaagcgtt gcgccgaaag agtttcttgg gcacttcacc gttttcgcgc
    16621 tggcctgcgt tgtcggttat tacgtggtgt ggaatgtatc gcacgcgctg catacaccgt
    16681 tgatgtcggt taccaacgcg atttcaggga ttattgttgt cggagcactg ttgcagattg
    16741 gccagggagg ctgggttagc ttccttagtt ttatcgcggt gcttatagcc agcattaata
    16801 ttttcggtgg cttcaccgtg actcagcgca tgctgaaaat gttccgcaaa aattaagggg
    16861 taacatatgt ctggaggatt agttacagct gcatacattg ttgccgcgat cctgtttatc
    16921 ttcagtctgg ccggtctttc gaaacatgaa acgtctcgcc agggtaacaa cttcggtatc
    16981 gccgggatgg cgattgcgtt aatcgcaacc atttttggac cggatacggg taatgttggc
    17041 tggatcttgc tggcgatggt cattggtggg gcaattggta tccggctggc gaagaaagtt
    17101 gaaatgaccg aaatgccaga actggtggcg atcctgcaca gcttcgtggg tctggcggca
    17161 gtgctggttg gctttaacag ttatctgcat catgacgcgg gaatggcacc gattctggtc
    17221 aatattcacc tgacggaagt gttcctcggt atcttcatcg gggcggtaac gttcacgggc
    17281 tcggtggtgg cgttcggcaa actgtgtggc aagatttcgt ctaaaccgtt gatgctgcca
    17341 aaccgtcaca aaatgaacct ggcggctctg gtcgtttcct tcttgctgct gattgtattt
    17401 gttcgcacgg acagcgtcgg cctgcaagtg ctggcattgc tgataatgac cgccattgcg
    17461 ctggtattcg gctggcattt agtcgcctcc atcggtggtg cagatatgcc ggtggtggtg
    17521 tcgatgctga actcgtactc cggctgggcg gctgcggctg cgggctttat gctcagcaac
    17581 gacctgctga ttgtgaccgg tgcgctggtc ggttcttcgg gggctatcct ttcttacatt
    17641 atgtgtaagg cgatgaaccg ttcctttatc agcgttattg cgggtggttt cggcaccgac
    17701 ggctcttcta ctggcgatga tcaggaagtg ggtgagcacc gcgaaatcac cgcagaagag
    17761 acagcggaac tgctgaaaaa ctcccattca gtgatcatta ctccggggta cggcatggca
    17821 gtcgcgcagg cgcaatatcc tgtcgctgaa attaccgaga aactccgcgc tcgtggtatt
    17881 aatgtgcgtt tcggtatcca cccggttgcg gggcgtttgc ctggacatat gaacgtattg
    17941 ctggctgaag caaaagtacc gtatgacatc gtgctggaaa tggacgagat caacgatgac
    18001 tttgctgata ccgataccgt actggtgatt ggtgctaacg atacggttaa cccggcggcg
    18061 caggatgatc cgaagagtcc gattgctggt atgcctgtgc tggaagtgtg gaaagcgcag
    18121 aacgtgattg tctttaaacg ttcgatgaac actggctatg ctggtgtgca aaacccgctg
    18181 ttcttcaagg aaaacaccca catgctgttt ggtgacgcca aagccagcgt ggatgcaatc
    18241 ctgaaagctc tgtaaccctg acggcctctg cggaggccgt cactctttat tgagatcgct
    18301 taacagaacg gcgatgcttt gacctcccgc tgtttgttca agcgcaattt tgacaataat
    18361 tgtcaacggc acggaaagca gcatacccac cggtcctaac aaccatcccc aaaaaatcaa
    18421 cgacaaaaat accaccaatg tcgaaagccc cagcccacgt cccatgatgc gcggctccag
    18481 aatattgccg aagaccagat taatcagcag atatcccgcc agcaccagca acgcttcgta
    18541 gaagccatta aacaccagta cctgagcgat aggggggatt gccgcgagaa ctgaaccaat
    18601 attagggatg taattaagcg caaaggccag caatccccag acaaaagcga agcgaacatc
    18661 gagtgcggcg agcatcgccc aggcgaccag gccggtgatg atgctgatgg ctgttttcag
    18721 caccagataa tgagaaacac tgtcaatcgc acgttgaatc gccgccatcc cttcaaccgg
    18781 acgcgccatc atttgctgaa attttccggg caattgtggc acttcgagca gcataaacag
    18841 caccgtcagc agcaataaaa atattgatga catggcatta gataactgcg tcaataagtt
    18901 ggtgagcaac gtcatcgccg cgttcggatc aatgtaatgc gccagttggt caactgagac
    18961 gtcaatccct acgcgttgca acaacggctc aagagcttgc agcggcgtca taatagagtt
    19021 gcgatattgc ggtaacgtcc gcgtcaactc gttgagcgcg gaacccagat aagctaatag
    19081 caacaccatc gccatcacga tgattgtcat caaaatcgac actgccagta cacgcggcac
    19141 acgccagcgg accatatgtt gcaccagcgg gttaagaata acagcaataa ataatgcgag
    19201 aataaacggc acgatgatct cggcggcaaa acggataccg cagagaataa tgaccagcat
    19261 tcccaacata atgacgattt ttaggccatt gagcgtgatg atcggctttg ccatgcttgt
    19321 ccccgttttt cttcctttgc taataataat gtctttttcg ctccgtcatc ataacgaaaa
    19381 tcaaaccgct caggtaaaga agtgaaaata ttttgagtta attcttaagc tatgatacaa
    19441 atcaggcgtg ttcaactacc gaggacaatt atcatctgcg atgacgagaa gcaacactgc
    19501 ggataattgt aatattatgg acaatttgtt caggacgtta ttttctacat ttacccatct
    19561 tcgtacctca tccactatct tgctcgtagg tgagcagcac tggcgcaacg cgctttagtc
    19621 ctttcttctg cctaagcttg ccctatgcgc agggcttgac cactttactt tggtttcgct
    19681 gaattaagcg aaaattaaaa tagttctctt gcaggagaag gacaatgtat atttattgga
    19741 ttttattagg tctggctatt gctacagaaa ttaccggtac gctgtcaatg aaatgggcga
    19801 gcgtcagtga gggaaatggc ggctttattt taatgctggt gatgatttct ctgtcgtata
    19861 tatttctctc tttcgccgtt aaaaaaatcg ccttaggcgt agcttatgcg ctgtgggaag
    19921 gtatcggtat tttatttatt accttgttta gcgttttgtt attcgacgaa agtttatcgc
    19981 tgatgaaaat tgccgggtta accaccctgg tcgccgggat agtgttgatc aaatcaggta
    20041 cccgtaaagc gcgtaaacct gaactggagg tgaaccatgg cgcagtttga atgggttcac
    20101 gccgcctggc tggcattggc aatcgtgctg gaaatcgttg ctaacgtctt tttgaaattt
    20161 tctgacggct ttcgtcgcaa aatatttggc ttgctctccc tggcggcggt gctggcggcc
    20221 tttagtgcgc tttctcaggc cgttaaaggg atcgacttgt ctgtcgttta tgcattgtgg
    20281 ggcgggtttg gtattgccgc cacgttagcc gcaggttgga tcttgtttgg tcaacggtta
    20341 aatcgtaaag gctggattgg cctggtcttg ctgttggctg gaatgatcat ggtgaaactt
    20401 gcctgatgaa gacgctgccc gcgctgtcgg gcagcgtttg aacattattt ttgcgacagt
    20461 tgatccagct tgtcgcgaaa accggtaaca gaaatggccc ggttatcggc gcgccagcga
    20521 tctttcgcgg caggagccga actttgcacc ccaattaatt gccagccgtc atcggtatgc
    20581 aacatcagag gcgaaccgct gtcacccggc aaggtatcgc actgatgtga catcaccgac
    20641 gtttgcgccc agccagtcac ttcacagttt tgatgactgt ataacgtatc gagatgatct
    20701 tcagggtatc ccgcctgagt cactttacga ccagccgatt ttaatgcggc agtaagcgcg
    20761 gctttatctc cctcaaataa cggcaacggt gtaatgccag aagggggatt acgtagcaca
    20821 atcaatccga agtcccacgg tgcagctgcg ggaggaacaa tccaaccatc cccatctgct
    20881 tttaaccgct ttcccagtgt cggatcaacg cggccttcta tgtcgtggat ctcatagcgc
    20941 caaagacctt tatttgacac aaaacgcagc gctaccgcct tatccgcttt acccttaggc
    21001 ggcgtcaata aacagtgtcc tgccgttaat gccagattgg gtgcaatcag cgtcgccgta
    21061 cataaattgc cgctggccgt ttccagttgc ccaaccgcat cccacggtga ttgggtcgtg
    21121 tcattcactg gcacacgatc atcatgacca aaaaacaggg tgctgacctc atcgtttgcc
    21181 gatttggcaa cgtctggttt atctgcaaac acaaaagcag acgtcaaact aattgcaccc
    21241 aacactacag caatggttgt acgcatatca cactctggtg ggtaattatg attattaaaa
    21301 gcaaaccctc ataaatacta tagacgggac gggatgaaag tgggagtaaa atcagataac
    21361 tacagtcagg aaagataaaa acgggcggca atcagtgcgc ataaaataag gatgataaga
    21421 atgaactcaa agcgataacg gcccaccata ctaccctccg ctaaaggcgg tgctaaacgt
    21481 tacccgctta gcaccgtaat tttcaggcgc gagggggcgc gccagcatta ctgttgaaaa
    21541 cttacgctgc gggttgtgca gcaggttttg ccggttgctg atggccgtgt ttcccggcgt
    21601 gtttcttcgc tgcctgcgct ttttgttcag gggctttctg ttcagctttc gtatttttat
    21661 gatgcttttt agccgcctgc gctttctggg caggtgctgc tttatgctgt tttttatgat
    21721 gtgtagtttt cgctggcgct gctttggtgg tcgtcgcagt cggagcaggt gtggtcgcag
    21781 tctctgcagc aaaggcggca gaagacagac ccatagcagc ggcaacaacc agagctaata
    21841 cttttttcat tgtcataccc tcaatttgtt ttttcattta accccactgc ggggccgttg
    21901 aaataactat atccctggga atggcagact tccgtgagtg gttggtttca gcgtgtaacg
    21961 atatgtacaa acgctgaata aattacagcg ttgataataa cgtattgtga cttaaggaaa
    22021 atttagctac caataatagt agtcttgatc gggttaacta ttatactact tacaaagcgg
    22081 tagaataact gcgcatcagt aataaatgac acacagcaaa atgaatccgt ttatttgggt
    22141 acttataatc ctgatgacgc tagatgcgct gcgggaattg gctggcgctt cgtctatttt
    22201 aggatggcta ttaacgttgg tttgagcagg caataagtcc ggacgggtat ttaccgcagt
    22261 ccggacttat ttttcaggcg tgcagacgac gatgcaaacg cgtcccgacc agcagagcaa
    22321 tgaccagcat cagagcaata aatgcgccga cgccgttcca gccatagtta tgccagaaaa
    22381 caccacccag cgtcccggca atactcgacc ccagatagta actgaacaga tacagcgagg
    22441 aggcctggcc tttagcgcgt tttgcgcggg ggccgatcca gctgctggct actgagtggg
    22501 ctgcgaagaa tccggctgag aagagtaaca ttccggcaaa gatcagccac agcgagctaa
    22561 ataaggtcat cagtaaacca aacggcataa ctcccgtcga aaacaacatc accggaccgc
    22621 gcccatagcg ggtggtcatg gttccggctt tgggtgagct ccatgtaccg gtcaaataag
    22681 ccagcgataa taagccaacc acggcctgac tgacatgcta gggggaaagc atcaaccgat
    22741 agccgatgta attaaacagc gtgacgaacg accccatcag caaaaagcct tctgcgaaca
    22801 ataacggtaa tccccggtca cgccagtgca gacgaaagtt gataaataac gtcttagggc
    22861 gcagcgaagt cgggcaaaaa tggcgtgatt cagggaggat tttccagaac atcaacgccg
    22921 aggccagcgc gaaacaaccg attgccgcca gagcaattcg ccagttgaaa aagtccgtga
    22981 agacaccact aattaagcgt ccgctcatgc cgccaattga gttgccgctg atatacaacc
    23041 ccatcgaaaa ggccacgaaa ctggggtgga tttcctcgct aagataagtc atgccaacag
    23101 ctgccacgcc acttaacgaa agcccaatca aggcgcgcat aatcaaaatg ccgtgccagc
    23161 tggtcatcat tgtcgaaagt aacgtacaaa tggaggccaa cagtagcgcc gtgaccatca
    23221 ctggtttgcg accaatggca tcggatagcg ggccagtaaa tagcaaaccg atagccaaca
    23281 tcgccgtgga aatggacagt gaaatactac tgttcgcggg cgttaagcca aactcctgcg
    23341 aaagcaccgg aaggataggc tgcacacaat agagaagtgc aaaggttgcc agtccggcag
    23401 agaacagcgc cagggtgacg cgcataaatt gcggcgtacc gcgtttaata aattgatttg
    23461 gctgagaaat gctttgcttg tcagtgtcgc ttgccggagc gccatcaaca gttgtagtac
    23521 ggctcacttg aaatccttgc taaatatgcc tgtggatcag gcttatacat agggtaggaa
    23581 aatcgaattg ttctgtctaa tatattaata atctcaaata agatgtttta aatatgaata
    23641 ttgaacttcg tcatctgcgt tactttgttg ctgttgcgga agagctgcat tttgggcgcg
    23701 cggctgcccg cctgaatatt tcgcaaccgc cgctaagtca gcagattcag gcactggagc
    23761 aacaaatcgg tgcccgactg ctggcacgaa ctaatcgcag tgtattgctg acggcagcag
    23821 gaaaacagtt tcttgcagat agtcggcaaa tcctgtctat ggtggatgac gctgccgctc
    23881 gcgctgaaag actgcatcag ggtgaagcgg gggagttgcg tattggtttt acttcgtccg
    23941 ctccttttat tcgggcggtg tccgatacgt tatcgctgtt tcgccgtgat tatcctgatg
    24001 tccatttaca aacccgcgaa atgaacactc gcgagcaaat cgctccgctc attgaaggaa
    24061 cgctggatat gggattgctg cgtaacacag cgttaccgga gacgcttgaa cacgcagtca
    24121 tcgtccatga accgcttatg gcgatgatcc cgtacgatca tcccctggca aataatccga
    24181 gtgtaacact ggctgaactg gctgaactgg ctgaactggc gaaagaaccc tttgtctttt
    24241 ttgatccgca cgtcgggact gggctgtatg acgatattct cggactgatg cgacgttacc
    24301 atttgacgcc cgtcatcact caggaggtgg gcgaggcaat gaccatcatc ggtctggttt
    24361 ccgccggtct gggtgtttca attttgcctg cgtcatttaa acgtgttcag ctcaacgaaa
    24421 tgcgctgggt gccgattgct gaagaggatg cggtttctga aatgtggttg gtctggccga
    24481 aacatcatga acaaagtcca gctgcgcgta actttcgtat tcatctgctg aatgctctca
    24541 ggtgagggaa atttcagcga aaaagcccga aaaatgtgct gttaatcaca tgcctaagta
    24601 aaaatttgac gacacgtatt gaagtgcttc accatagcct acagattatt tcggagcgcg
    24661 aaaatatagg gagtatgcgg tggttgctga aaaccagcct gggcacattg atcaaataaa
    24721 gcagaccaac gcgggcgcgg tttatcgcct gattgatcag cttggtccag tctcgcgtat
    24781 cgatctttcc cgtctggcgc agctggctcc tgccagtatc actaaaattg tccgtgagat
    24841 gctcgaagca cacctggtgc gagagctgga aatcaaagaa gcggggaacc gtggccgtcc
    24901 ggcggtgggg ctggtggttg aaactgaagc ctggcactat ctttctctgc gcattagtcg
    24961 cggggagatt ttccttgctc tgcgcgatct gagcagcaaa ctggtggtgg aagagtcgca
    25021 ggaactggcg ttaaaagatg acttgccatt gctggatcgt atcatttccc atatcgatca
    25081 gttttttatc cgccaccaga aaaaacttga gcgtctaact tcgattgcca taaccctgcc
    25141 gggaattatt gatacggaaa atggtattgt acatcgcatg ccgttctacg aggatgtaaa
    25201 agagatgccg ctcggcgagg cgctggagca gcataccggc gtaccggttt atattcagca
    25261 tgatatcagc gcatggacga tggcagaggc cttgtttggt gcctcacgcg gggcgcgcga
    25321 tgtgattcag gtggttatcg atcacaacgt gggggtgggc gtcattaccg atggtcatct
    25381 gctacacgca ggcagcagta gtctcgtgga aataggccac acacaggtcg acccatatgg
    25441 taaacgctgt tattgcggga accacggctg cctcgaaacc atcgccagtg tggacagtat
    25501 tcttgagctg gcacagctgc gtctcaatca atccatgagc tcgatgttac atgggcaacc
    25561 gttaaccgtg gactcattgt gtcaggcggc attgcgcggc gatctactgg caaaagacat
    25621 cattatcggg gtgggcgcgc atgtagggcg cattcttgcc atcatggtga atttatttaa
    25681 cccacaaaaa atactgattg gctcaccgtt aagtaaagcg gcagatatcc tcttcccggt
    25741 catctcagac agcatccgtc agcaggccct tcctgcgtat agtcagcaca ttagcgttga
    25801 gagtactcag ttttctaacc agggcacgat ggcaggcgct gcactggtaa aagacgcgat
    25861 gtataacggt tctttgttga ttcgtctgtt gcagggttaa cattttttaa ctgttctacc
    25921 aaaatttgcg ctatctcaat ttgggccagg aaagcataac ttagactttc aaggttaatt
    25981 attttcctag tttatatttg tgaagcataa cggtggagtt agtgatgctg aagcgtttct
    26041 ttattaccgg tacagacact tctgtaggga aaacggtggt ttcccgcgca ttgctacaag
    26101 cgttagcctc ccagggaaaa agggttgcgg gatataaacc cgtagcgaag gggagcaaag
    26161 agacacccga agggctgcgt aataaagatg ccttggtgtt gcagagtgtt tcaaccatcg
    26221 aactgcctta tgaagcagtt aatcctatcg cgttaagcga agaagaaagt agcgtggcgc
    26281 acagttgccc aatcaattac accctcattt caaacggcct ggcaaacctg accgataaag
    26341 tcgatcatgt cgtggtagaa gggactggcg gctggcgcag tctgatgaat gatttgcgtc
    26401 cactctctga atgggtagtg caggaacaac tgccggtgtt gatggttgtc ggtattcagg
    26461 aaggttgcat taaccatgca ctgctaacag ctcaggcgat cgccaacgac gggctgccgc
    26521 tcattggctg ggtggctaac cgaatcaacc caggactggc gcattatgcg gaaatcattg
    26581 atgtgctggg taaaaaactt ccggcaccgc tcattggtga actgccttat ctgccgcgcg
    26641 ccgaacagcg tgaactgggg caatacatcc gcttagctat gctgcgcagt gtgctggcgg
    26701 tagatagagt cacggtgtaa cgtccgcgaa attaccgacg caattacgca ggcaatcaat
    26761 aaaccgggga gtagctgata ctccccggtc atttcacata tcatcaacgt cgacataatc
    26821 ggcgcgtgcg tggttgccgc caacagtgtc gccatcccgg tcaatccgag taaaagcgta
    26881 atttcttcgc catcagggaa ccataatccc aggctacgac catacaacat gccaatggca
    26941 agaccgataa atagcgtcgg tgtaaagaca ccaccgggtg cgccggaacc gctactcgcc
    27001 agcacggcac acagtttaca aaggaagatc ccggcaatga tcattaacag tggtggggcg
    27061 gttaaaaagg attgtacggt gctatagccg ttgccccaca ctgcaggtgt gaacagggaa
    27121 agcagaccca cgatcaaccc gcctagtgcc agttgccagg gcggcgcaag tttgagactc
    27181 acaaatccac gatgacaggc gttcattaac gttaacaaca gtggtccgca cagacctgcc
    27241 agcacacctg tactgataat cagcgcatag tcacgagcct gaaccgtcac tgagagttgt
    27301 acgttgtaga gtaacgcgtc gctatgatta atcagattgc taaccagcca tgccacgatg
    27361 gcggaaataa tcaccgggcc gagagaggcc aacatcatag tgccaaacag cacttcggct
    27421 ataaataaac tgccagcaag cggggcacga taggccgcag ccattcccgc cgcggcccca
    27481 caggcgatcc ataatttcca ttcctggcgt ggcgtaaaac gttgggcaaa acaggaggcg
    27541 gcaagggcag ctaaaagaat catcgcacct tcgcgaccaa ttgcactgcc gctggttact
    27601 accagcagcg aggcaagcga tttaaccagg cttgctgcgt aatcgaactg tccatcggtt
    27661 tgcaacgctt ccatgtaatc ggtcggcgca tgagggcgtt gttgggtaaa tttctgccag
    27721 cccatcagca acaaacccgc cgccagtccg ccgagcgccg gagttagcag ccgtcgccag
    27781 gaggaaaggt ttgtcgctgc attgaccaga ctgccggagt cattattgag gaacaaccac
    27841 tccagtagca gcatcgcatg acgaaacccg gcaacggcaa agacagcgag aataccgacg
    27901 actgttgcga taagcagacg gcggaacata gtgcgcagat ccggatagat gtgtagatga
    27961 tgcatgagca gaaatgggca atgaaacacg ggcgactatt ttgcggcaaa agaggccaac
    28021 cgcaaaagca gtgcgtgctt taagagataa aaaaacgtga cattgtaacg tattattgcc
    28081 gaatcgtgcg taatcgtcac catccggcaa tattacggtg atcctatcga aagagtggtt
    28141 taaccgcgac aggaatcaac agttgtgatt gccattgcgc cagcgttaag cgagccagtt
    28201 cacccagtgc acggtagaaa gggtgttctg ctttttcgat aaaaacatca agaaaacgtg
    28261 ttgaccacgg aaaaaggtgc catgccagca attcttcgca ttctgtctgg cgaccattct
    28321 ccgccaacca cgcagccatc aacagcagtg aaccaaaatg atcttccggt tcgttttgct
    28381 tcatttcaaa ctgaatgcct ttctcgcgca tccactgacg aagtgccaat gttgaatcgc
    28441 caaacagcac agattcgcga tccagccaga ccgaccccca cggcggagac ggcagtgccc
    28501 acgggccgac aaacaaacgc tgccaggcct gggcgtgagt ctcttcacac tgtgtctgaa
    28561 aagccgtcac cagcggcgct aatgacgctt ctggtagagg ccactgagtt tcccagccgt
    28621 cactggtgag taccgcaacc agaggtgcgg cttccgcact ctctggagcg taataaaaca
    28681 gcgcgcccaa cacgcgcgcc gcgacagaaa aattatcttg ctgtgaaaaa tgggtcattc
    28741 ctaacatcct gaaatgtgcg ggttgccccg caccaatttg ttaacctgca atagccattc
    28801 ctactgtcat gtgcaggcca taaaagagca cacgcccgat catctcacca acaagtatga
    28861 ggattagccc cagaacaagt ccggcaacgt gaggttcacg gcgacgaatg agcggacaaa
    28921 gccagcatcc aagacctgcg cataacaata ccacacgcca gacctgtaag gaggcgtaat
    28981 ctggtaccag tgcgctggct tgttgcacgg atgagtgaat agacgccaga gacaaacctt
    29041 gcagcacaat cacctcggca caagcgatca gcgccagaac gctaatgatg gcaaatggtg
    29101 tggtattaaa tgtaacgcgg gctgcccgca gaattgcggc agcaagtatt ggaccgctca
    29161 acaataccgt caggaagaaa gccagcgtag tgtaaccgtt atgccaggtt ggcacggtgt
    29221 cgatttgata cacgcaggtc atcatccaga cgaaaatgac gccgagcgcc atgctgacaa
    29281 gtaaccagag tttccccaac gcttgcggca ttttaccgat gaccgccacc agccaccata
    29341 gtccgccaac ggcaaaaaat atggaaccgg cagcaatctc gttactcaga ccagaagctc
    29401 cgattcgatt cagcgagtta aacgctcgca gtggcgatcc caggtgcatg acagaggcaa
    29461 taaacccgat gcccatcaac agccagagga aaaacatgcc acgaacaatg tgctgacggt
    29521 ctgcgtcatt tttggcggcg aaccagccaa ttccgctaac aatcagcgcg cctactacgc
    29581 attgacccaa tacggtaaag ataaccagtg gccattcatg ccatccattt cccattttac
    29641 acttcctccg gattagccag ataaccagtg gtatcgccgg tcgggcggct gttggcgtta
    29701 ggtttgataa cgatattggg ttttgtgaaa tgcgcgcgcg gcagtggcgc gacggcagcc
    29761 agagtgccgt gcttctggcg cagctcttca attggaccga actcaagtgc acgcagcggg
    29821 caggattcga cgcatatggg ttgtttcccc tcggcgacgc gcgaataaca accatcgcac
    29881 ttcgtcatgt gccctttttc agcattgtac tgtggtgcgc cgtacggaca agccatgtgg
    29941 cagtagcggc agccgataca aacatcttca tcaaccacca caaagccatc ttcacgctta
    30001 tgcattgcgc cgctggggca gacttttgta catgcggggt catcgcaatg gttacaggag
    30061 atggagagat aataagcgaa cacgttctgg tgccagatgc cattatcttc ctgccagtcg
    30121 ccgccagcat attcataaat acggcggaaa ctgacttccg ggccaagatc tttaaaatct
    30181 ttgcacgcca gttcgcaggt tttacagcca gtacagcggc tggaatcgat aaaaaatcca
    30241 tattgtgtgg tcatgggcta ctccttaaac cttttcgatc tggacaagat tgctgtgcga
    30301 cgggtttccc tttgccagcg gtgaagggcg gtgagaggtc agaatattga tactgccgcc
    30361 gtgatcgacc cggtcaccaa acatatccgc tttaagccac gcaccttgcc cgatggcggt
    30421 aacgccaggc agaatacgcg gagtcacttt tgcggcaatc agcatttctc cattattgtt
    30481 aaatacccgc acggtatcgc catgacggat accgcgtgcc tgagcatcaa tggggttgat
    30541 ccacacctct tgtgggcagg cctgctgtaa cacatcaata ttgccgtagc tggagtgggt
    30601 acgcgctttg gtttggcgac ttcactcagc atccagtaga tgggtttgcg ttcaaatttt
    30661 gctgaggttg cgggttgggc gaggatcaca tagcccatat tccctgcaga ttcatgagag
    30721 ataaggtctt cttgctctgt tggcatcagg tcaggcaaca ggatatcgca atacttagcc
    30781 gaggccgtca tgaagtggtc aatgccaaca atcatctcgc acttgctgtc atcctgaagc
    30841 acctcatggg tgtgattgat gtcgccatgt tgattgatca atgtgttact ggcgtagcac
    30901 cataaaaact tgatggggac atccagtttt tcttttccac gaacaccatc acgggtcgcg
    30961 gtcattttcg taccatggtc gatggcatct gtccatgtaa aaacggaaat ctgcgtttta
    31021 acaggattct cgagcatcgg gaaccattct acccccagat cccagctacc ttcgcgtaca
    31081 cctgagttgc cgccgtttat gccgacgtta ccggtaagaa cggaaagcat ggcaatagcg
    31141 cgggacgttt gttcgccgtt ggagtgtcgt tgtggccccc aaccctgaca aatataagca
    31201 ggttttgctg aaccgatctc tcgtgccaac tggataattt tttctgccgg gatgctggtg
    31261 atttttgctg cccattccgg cgttttagct atgccgtcag gcccttcgcc cagaatatag
    31321 gctttataat gcgcgttacg tggtgcgttg gcgggcagcg ttttttcatc gtaaccaaca
    31381 caatatttgt cgagaaatgg ctgatcgacc atgttttcag taatcagtac ccaggcaatc
    31441 gcacaggcca gtgcgccatc ggtgccaggg cgaatgggca gccattcatc ttcacgcccg
    31501 gcagctgtgt cgttatagcg tggatcgatg acgatcatgc gtgcgtttga acgttcgcgg
    31561 gcttgctcga cgtagtaagt gacaccaccg ccgctcatcc gcgtttctgc cgggttattt
    31621 ccgaacataa cgaccagttt cgtattggcg atatcatccg ggctgttgcc atcattggca
    31681 ccgaacatat aactcattgc ggcactgatc tgtgcggtac tgtagctgcc atagcgactg
    31741 agaaaacccc cgcaagagtt catcagacgg tacgggacgt tcgagttggt gatatttccg
    31801 ccatctacgc ctgttccgta caggacatgt acagcctcat tgccgtaatc tttcaggatc
    31861 cgccgaagat tatcactgat ggtatccagg gcttcgtccc aacttatccg ttcaaattta
    31921 ccttcaccgc gctcgccgac gcgcttcatg ggatatttca acctgtcagg atgattcatc
    31981 cgtcggcgga tagagcgccc gcgtaaacat gcccgaacct gatgattacc gtaaacgtcg
    32041 tcacctgtcg tatcagactc cacccagtac acggtgtcat ctttcacatg caaacgtagc
    32101 agacagcggc tcccgcagtt aacggtgcag gaactccaga ccgttttctc ttctaccgga
    32161 gcctctgccg cccggaccat ttgggaaaat ggcagagtga aagcactgct tgccagcgcg
    32221 agactgccaa gtgcggaggt tttcatcaga cttctatggc tgatttcagc cttcatgagc
    32281 gcctctgtgg tatggatttt catcattact cacttattgc ttttcaaaca aaatgtcatg
    32341 ccagaattta tggttgtagt gggttatatt ttttcgatct cgaccagatt agtgtgctgc
    32401 gggtttccct tcgccagtgg tgaagggcgc agagtggtta gcgtattcac acatccgcca
    32461 tggtcgattt tatcgccaga catattgggc tcgtgccagg ctccctggcc catagcgcta
    32521 actccaggga gaatgcgtgg tgttactttg gctggtagcc gaacttcgcc acgatggtta
    32581 aacacccgca ccatatcgcc gttggcaatc ccacgtttct gtgcatctat agggttgatc
    32641 cacacctcct gacggcaggc tgccttcagg acatcaatgt tgccgtaggt cgagtgagta
    32701 cgggatttgt aatggaaacc aaacagttgc agtgggaagg ttctacgttc aggggagtcc
    32761 cagccttcaa aggttgaggc ataaactggc agtgggctta tcacttcatc tttttccagt
    32821 tcccaggtac gggcaatttc cgccagcttg ctggaataaa tttcaatctt accggaaggc
    32881 gttttaagtg gatttgcctc ggggtcgtca cgaaatgctt tgtaggcgac aaaatggcca
    32941 ttgggatctt tacgcttata gatacccatt tttttcagtt cgtcgtaaga cggtaacgcc
    33001 ggatctttgg caagcatttt ggcgtacaga tgttgtaacc attgttcctg cgtgcgacct
    33061 tctgtgaact tttgatagac gtcaggtcca agacgtttcg cgacttcact caggatccag
    33121 taaatcggtt tacgttcgaa tttttcgctg gtgactggct ggaggaaaat gagatatccc
    33181 atgttaccgg cgtagtcgtt aggaataata tcttcctgct caacggtcat caggtcttcc
    33241 agcagaatgt cggcatattt tgccgatgag gtcataaagt tttcgatgac cacaatcatt
    33301 tcgcatttcg attcgtcctg cagaatttca tgcgttttgt tgatgtcaga atgctgatta
    33361 acgagggtat ttcccgcgta gttccagatg gactccattg ccagacggat ggatggactt
    33421 gaagtagttg actcaatgct acttaaggtc ctgatatgac tgacattaat catgataaca
    33481 ctgtatatcc atccagttct ttctctgggt ctccctatcg gtatcagcaa gtctacacct
    33541 caagccctga cacaaggctg acacaaatca atctactcag cgtagctaag cctcagttag
    33601 tccgcagagc caatggtcga tatactatcc gcttcagact caagggacag accacaccgt
    33661 ttctttcagt atcgacccgc agcacagaca ggagagtagc cacaatgcgt cagagagagc
    33721 ttgcagccac cgctaaggcc ttcatgttgg ataggcctga agtctcacta caggagctta
    33781 cagagcatct ccggtctatg gctgagcagt tcctgacaga cgctagtgac gattactgga
    33841 atggacttga ggtcgctact ctggtcgacg agaagtccaa cctgaaggag ctagccgcta
    33901 cgcaagcctt gagtctggac cagcagaagg gcataaggtt agcgctggaa gtcctgaccg
    33961 cagcacagca gagagtcgac actggtgata cctctggtct gattaagctg attgatgata
    34021 ataaccttac tgatgactca actataggcg actctacgtc aattctgaat aatgagcagg
    34081 gggatagacc ggcagttttc acacaggagc gccagtcatc tgtagttttt tcaagcctag
    34141 tgtcctcgct gctggctgag aaggtccaga ccctgaagac atccagctac aaggacctct
    34201 cgtcctcact caatactgtc tcccggttcc tgcctgaaga catggacctg atgtcccgct
    34261 ctgggtggct ggctgtcagg gactctatgc tggcttctga ggtgagacca tcgacaatca
    34321 acaagctgct gaccaaagcg aagatgtgtc tcgattatgg cctgatgaat gggcaacttg
    34381 agggccgcaa ccctattgaa cggatgaagc tgaccaagga tatcgactcc aagcgcagag
    34441 cgttcacaga cgaggagctg gagagacttc tggtccgtgt tgagt
//
LOCUS       DAJTSI010000028        34446 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000028 DAJTSI010000000
VERSION     DAJTSI010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34446
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.028"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..333)
                     /locus_tag="OO327_001214"
     CDS             complement(<1..333)
                     /locus_tag="OO327_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6017828.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQK"
     gene            703..1653
                     /gene="corA"
                     /locus_tag="OO327_001215"
     CDS             703..1653
                     /gene="corA"
                     /locus_tag="OO327_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR6017829.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1694..2074)
                     /locus_tag="OO327_001216"
     CDS             complement(1694..2074)
                     /locus_tag="OO327_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HCR6017830.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2088..2504)
                     /locus_tag="OO327_001217"
     CDS             complement(2088..2504)
                     /locus_tag="OO327_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6017831.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2563..3453)
                     /gene="rarD"
                     /locus_tag="OO327_001218"
     CDS             complement(2563..3453)
                     /gene="rarD"
                     /locus_tag="OO327_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR6017832.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3505..3972)
                     /locus_tag="OO327_001219"
     CDS             complement(3505..3972)
                     /locus_tag="OO327_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR6017833.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4137..5006
                     /gene="pldA"
                     /locus_tag="OO327_001220"
     CDS             4137..5006
                     /gene="pldA"
                     /locus_tag="OO327_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR6017834.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5139..6968
                     /gene="recQ"
                     /locus_tag="OO327_001221"
     CDS             5139..6968
                     /gene="recQ"
                     /locus_tag="OO327_001221"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR6017835.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7032..7652
                     /gene="rhtC"
                     /locus_tag="OO327_001222"
     CDS             7032..7652
                     /gene="rhtC"
                     /locus_tag="OO327_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCR6017836.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7714..8334)
                     /gene="rhtB"
                     /locus_tag="OO327_001223"
     CDS             complement(7714..8334)
                     /gene="rhtB"
                     /locus_tag="OO327_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR6017837.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8445..9467
                     /gene="pldB"
                     /locus_tag="OO327_001224"
     CDS             8445..9467
                     /gene="pldB"
                     /locus_tag="OO327_001224"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR6017838.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9475..10275
                     /gene="yigL"
                     /locus_tag="OO327_001225"
     CDS             9475..10275
                     /gene="yigL"
                     /locus_tag="OO327_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR6017839.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGISKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10351..11250
                     /gene="bioP"
                     /locus_tag="OO327_001226"
     CDS             10351..11250
                     /gene="bioP"
                     /locus_tag="OO327_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR6017840.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11138..12091)
                     /gene="metR"
                     /locus_tag="OO327_001227"
     CDS             complement(11138..12091)
                     /gene="metR"
                     /locus_tag="OO327_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR6017841.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALGNFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12327..14588
                     /gene="metE"
                     /locus_tag="OO327_001228"
     CDS             12327..14588
                     /gene="metE"
                     /locus_tag="OO327_001228"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR6017842.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14627..15442)
                     /locus_tag="OO327_001229"
     CDS             complement(14627..15442)
                     /locus_tag="OO327_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6017843.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15704..16465
                     /gene="udp"
                     /locus_tag="OO327_001230"
     CDS             15704..16465
                     /gene="udp"
                     /locus_tag="OO327_001230"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR6017844.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16606..18033
                     /gene="rmuC"
                     /locus_tag="OO327_001231"
     CDS             16606..18033
                     /gene="rmuC"
                     /locus_tag="OO327_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR6017845.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18128..18883
                     /gene="ubiE"
                     /locus_tag="OO327_001232"
     CDS             18128..18883
                     /gene="ubiE"
                     /locus_tag="OO327_001232"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR6017846.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18897..19502
                     /gene="ubiJ"
                     /locus_tag="OO327_001233"
     CDS             18897..19502
                     /gene="ubiJ"
                     /locus_tag="OO327_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR6017847.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19499..21139
                     /gene="ubiB"
                     /locus_tag="OO327_001234"
     CDS             19499..21139
                     /gene="ubiB"
                     /locus_tag="OO327_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR6017848.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21218..21487
                     /gene="tatA"
                     /locus_tag="OO327_001235"
     CDS             21218..21487
                     /gene="tatA"
                     /locus_tag="OO327_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR6017849.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21491..22006
                     /gene="tatB"
                     /locus_tag="OO327_001236"
     CDS             21491..22006
                     /gene="tatB"
                     /locus_tag="OO327_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR6017850.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22009..22785
                     /gene="tatC"
                     /locus_tag="OO327_001237"
     CDS             22009..22785
                     /gene="tatC"
                     /locus_tag="OO327_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR6017851.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22827..23609
                     /gene="tatD"
                     /locus_tag="OO327_001238"
     CDS             22827..23609
                     /gene="tatD"
                     /locus_tag="OO327_001238"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR6017852.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23606..24094)
                     /gene="rfaH"
                     /locus_tag="OO327_001239"
     CDS             complement(23606..24094)
                     /gene="rfaH"
                     /locus_tag="OO327_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR6017853.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24261..25754
                     /gene="ubiD"
                     /locus_tag="OO327_001240"
     CDS             24261..25754
                     /gene="ubiD"
                     /locus_tag="OO327_001240"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR6017854.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25800..26501
                     /gene="fre"
                     /locus_tag="OO327_001241"
     CDS             25800..26501
                     /gene="fre"
                     /locus_tag="OO327_001241"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR6017855.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26693..27856)
                     /gene="fadA"
                     /locus_tag="OO327_001242"
     CDS             complement(26693..27856)
                     /gene="fadA"
                     /locus_tag="OO327_001242"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR6017856.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDTQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27866..30055)
                     /gene="fadB"
                     /locus_tag="OO327_001243"
     CDS             complement(27866..30055)
                     /gene="fadB"
                     /locus_tag="OO327_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR6017857.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30245..31576
                     /gene="pepQ"
                     /locus_tag="OO327_001244"
     CDS             30245..31576
                     /gene="pepQ"
                     /locus_tag="OO327_001244"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR6017858.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31576..32190
                     /locus_tag="OO327_001245"
     CDS             31576..32190
                     /locus_tag="OO327_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR6017859.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32229..33680
                     /gene="trkH"
                     /locus_tag="OO327_001246"
     CDS             32229..33680
                     /gene="trkH"
                     /locus_tag="OO327_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR6017860.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33692..34237
                     /gene="hemG"
                     /locus_tag="OO327_001247"
     CDS             33692..34237
                     /gene="hemG"
                     /locus_tag="OO327_001247"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR6017861.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8108 a         8698 c         9302 g         8338 t
ORIGIN      
        1 cttctgctga ccgttttcga tgtacaaacc gagcggcgca tagctttcat catagatgcc
       61 gccgttcatc gccatctgca cctgaccctg actattaata tccgccagca gagcatgtaa
      121 cgttccccac gcttcgccat tggctttttg ccagtacatt ttcacccgct ctgtttgagg
      181 attaacggta tacgcctgta cggtcagcgt cggatctgag agtgcgcaat catcagcggc
      241 aacagcaaac aagggaagta aggtgagggt gagaaaaatc cgtttgagat tcaaggtgat
      301 cattccttta ccaatgagta gccgatgcgc cattataggt cctagatgtg ggattttttt
      361 atcctgttag cgaccttgac gagtaccaaa aagcgcgaag ttcaactatt gttctgtggt
      421 gttctgttgc gtgttgacgg caaaattttg ctggcgtaac atgcgcgcac gatcactcta
      481 agaggacatt cgccttggac acacccagta gatactggct cactatcctg tcatccagga
      541 tcaactccta aggctatccc tttttgctga tagccttagc ggttgtcagc gacctcaatt
      601 tttcccgtcg cgctgagtca ggctgtttaa tggtctgaaa cccaatttgt ttctgtgtgc
      661 ccaccgaact gtccgatatt ttaagcattg ggagtcccgg tcatgctgag cgcatttcaa
      721 ctggaaaata accgactgac ccggctggaa gtcgaagagt cacaacccct tgtaaatgca
      781 gtatggattg atcttgtcga accggacgac gacgagcgac tgcgcgtaca atctgaactt
      841 ggccagagcc tggcaacccg cccggaactg gaagacatcg aagcatcggc acgtttcttt
      901 gaagacgacg acggcctgca tattcactcc ttcttcttct ttgaagatgc ggaagatcac
      961 gccggtaact ccactgtggc atttaccatc cgtgatggtc gtctgtttac tctgcgtgag
     1021 cgtgaactgc ccgcttttcg tctgtatcgt atgcgtgccc gtagccagtc gatggtagac
     1081 ggtaacgcct acgagttgct gctggatctg ttcgaaacca aaatcgaaca gttggcagat
     1141 gaaattgaaa atatctatag cgacctggag cagttgagcc gggtgattat ggaagggcat
     1201 cagggcgatg agtacgacga ggcgctctcc acgctggcgg aactggaaga tatcggctgg
     1261 aaagttcgcc tgtgtctgat ggatacccag cgcgctctca acttcctggt gcgtaaagcg
     1321 cgtttaccgg gtgggcaact ggagcaggcg cgtgaaatcc tgcgcgatat cgaatccctg
     1381 ctgccgcata acgaatccct gttccagaag gtgaacttcc tgatgcaggc ggcaatgggt
     1441 tttatcaaca tcgagcagaa ccgcatcatc aaaattttct cggtggtatc cgtcgtgttc
     1501 ctgccgccaa cgctggttgc ttccagctac gggatgaact ttgagtttat gccagaactg
     1561 aagtggagtt tcggctaccc tggcgcgatt atctttatga tcctcgcggg cctggcaccg
     1621 tatctgtact ttaagcggaa gaactggttg taaaaagaag gcggtggctt agcctggcta
     1681 agccacctgt tattcaaagg ctccaggtat ttaacccttt tacctctttc tcataaaacc
     1741 atttgttcgt gttaacagca acataggctg ctacggcaat tcccagaatg ttaacgccaa
     1801 ttaatgcacc aacaaataga gatagaatgc caagtaatag aactataatt gcttttttcc
     1861 ataagcccag aacaaataaa tagatccagg aacagaagaa agcaatgaaa ttcatttgaa
     1921 tagttaatcg ttgtcttact tttaatgctt taaatgctgc tttatattct ggtgttgctc
     1981 cccagaatcc aggaaagcca tgttgatcat aaaaattaaa tcggtatttc catttttcac
     2041 ttaatgaatc atcgttcata tattccttac tcataaatac tccataacta ttgttttgat
     2101 gaatcagtag gtgcaagcat tagcatactg aaagtggaaa aataacaaat caaaaaaatc
     2161 atcgaaccat tgcctgaaca ggcaaaatct tcggctatca ttgtgatgat agagatgata
     2221 tatactgcta atgtaccaaa aacataaatt tttatatata tgaaaccact atcacggagt
     2281 cgttggcaat tcatgttgat gacgagataa tggagtgcga tggtagagac tataacaaga
     2341 aaacctgctt ccccatcgtt aaaaaagata ataagaaagg caaaaatgaa atttattaaa
     2401 ataaataaaa atatatatcg gtgtctggaa atcttaccgt tagatgttgg gataaacata
     2461 cgtaacataa attttacatc cttgtgtgag tctcctgtca gcatggcaat atgcccactc
     2521 tcatacaaga gtcggcatat tttttcagaa tatatttatt ttttatttgg acgttctacg
     2581 ctgcgtataa atcgcgtcca tcacaaaaat tgccagcgcc acccaaataa aggtgaaagt
     2641 caccatctta tcggcacccg gtttttcacc ataaaacgtc acagccagca ggaacatcag
     2701 cgtcgggcca atgtactgaa aaaagcctaa cgttgagaga cgcaggcgcg tggcagcggc
     2761 agtaaaacac aacagcggta cggtagtgac aataccggcg gcgatcagca gtaaattcag
     2821 cgacatcggg ttttgcccca tatggctggt tgaactgtcg gcaatagcaa acaggtaaat
     2881 cgccgccacg ggcagcagcc acatggtttc aattaacatg ccggtttgtg cttctaccgc
     2941 aatcttcttg cgcaccaggc cgtagaaggc aaaactaaat gccagcccca gcgcgataat
     3001 aggtagcgaa ccaaaagtcc acagctggac caacacgcca catatcgcca gaatcaccgc
     3061 cagccattgc atccggcgga atcgctcgcc gaggaaaatc atccccagca caatgttcac
     3121 cagcgggtta ataaagtaac caaggctcgc ttccagcata tggtgattgt tcaccgccca
     3181 gataaacagt agccagttgc caccaatcag tacggcagag actgccagca taaaaatttt
     3241 ctgtggcgtc tgaatcagcg tttttaaata ggaccactgg cggcaaatgc tcatcagcac
     3301 caccataaag aaaaacgacc agatcacgcg atgcgtcagg atctcatcgg cgggcacgta
     3361 gtaaatcagc ttgaagtatg ctggcgcaat accccaaata aaataagcgg caagagcgag
     3421 taatacgccc tgccgcgttt gttttgcatc catcgggaat actcattttt aattggtaac
     3481 agcagtttac ctgcttttat gtcttcaacc taccatatag gtggcggtgg cactggcaat
     3541 ataaagctgt tcttcattgt gtaattcaac gcgggcgacg gcgactttat tgcctgcacg
     3601 caacaggcta ctggtagcag taaaacgctc gcccctgcct gggcgcagat aatcaacgcg
     3661 aagatcaatg gtccccatcc gcgatagccg ctggcgtagt tcatcttcac tgatggtttc
     3721 gtggcgggtt aaggtacttc ccacgcacac cagaccggcg gcgacatcca gcgccgacgc
     3781 aatgactccg ccgtgcaaaa tgctttgcgc ccagttaccc accatcatcg gctgattttt
     3841 aaaggccagc tgtgcgaact ctttttcgta acgctccagt tccatcccca atgcgcggtt
     3901 aaatggcatg tgataaacaa acatctcacc cactaatttc agggcttgtt cagcggtcag
     3961 tacggcagac atatcatcct tacacttcat tggttaatga aatgttgatt ttatgcttct
     4021 ttgttgttgg tttctacttt aggaagggat aactaacggc aatggagtta agtatgtaaa
     4081 atagcccgca gaaaaacatt caccttatca ataattcgtt acggagaaca cgaccgatgc
     4141 ggactctgca gggctggttg ttgccggtgt ttatgttgcc tatggcagta tatgcacaag
     4201 aggcaacggt gaaagaggtg catgacgcgc cagcggtgcg tggcagtatt atcgccaata
     4261 tgctgcagga gcatgacaat ccgttcacgc tctatcctta tgacaccaac tacctcatct
     4321 acacccaaac cagcgatctg aataaagaag cgattgccag ttacgactgg gcggaaaatg
     4381 cgcgtaagga tgaagtaaag tttcagttga gcctggcatt tccgctgtgg cgtgggattt
     4441 taggcccaaa ctcggtgttg ggtgcgtctt atacgcaaaa atcctggtgg caactgtcca
     4501 atagcgaaga gtcttcaccg tttcgtgaaa ccaactacga accgcaattg ttcctcggtt
     4561 ttgccaccga ttaccgtttt gcaggttgga cgctgcgcga tgtggagatg gggtataacc
     4621 acgactctaa cgggcgttca gacccgacct cccgcagctg gaaccgcctt tatactcgcc
     4681 tgatggcaga aaacggtaac tggctggtag aagtgaagcc gtggtatgtg gtgggtaata
     4741 ctgacgataa cccggatatc accaaatata tgggttacta ccagcttaaa atcggctatc
     4801 acctcggtga tgcggtgctc agtgcgaaag gacagtacaa ctggaacacc ggctacggcg
     4861 gcgcggagtt aggcttaagt tacccgatca ccaaacatgt gcgcctttat actcaggttt
     4921 acagcggcta tggcgaatcg ctcatcgact ataacttcaa ccagacccgt gtcggtgtag
     4981 gggttatgct aaacgacttg ttttgatgaa cggttgagtg gttggcaaat ctggaatcca
     5041 gcatccagga ttaccctctc agagactaaa agcattgcag tttctcgcgc aggcgctgaa
     5101 aatagcgcct gtttttattt caggcaatcg gggtgaatgt ggcgcaggcg gaagtgttga
     5161 atctggagtc cggagctaaa caggttttac aagaaacctt tggctaccaa cagtttcgcc
     5221 ccggccagga agaaattatc gacactgtgc tttccggtcg cgattgcctg gttgttatgc
     5281 ccactggtgg cggaaaatcc ctttgctatc aaattcctgc cttattgcta aacggcctta
     5341 ccgtggttgt ttcaccgctg atttcgttga tgaaagatca ggtggatcaa ctacaggcca
     5401 acggcgtggc ggcggcgtgc cttaactcga cgcaaacccg cgaacagcaa cttgaagtga
     5461 tgacaggctg ccgcaccggg caaattcgct tactgtatat cgccccggaa cgcctgatgc
     5521 tggataactt tcttgagcat ctggcgcact ggaatccggt gttattagcc gtcgatgaag
     5581 cgcactgtat ctcccaatgg ggtcacgatt tccgcccgga atatgccgcg ctcggtcagt
     5641 tgcgccagcg gttcccgacg ctgccgttta tggcgctgac cgccacagcc gacgacacca
     5701 cgcgccagga tatcgtgcgc ctgctggggc tgaacgatcc gctgattcaa atcagcagtt
     5761 ttgaccgtcc gaatattcgc tacatgctga tggagaaatt caaaccgctc gatcagttga
     5821 tgcgctacgt gcaggaacag cgcggtaagt ctggcattat ctactgcaac agccgggcga
     5881 aagtagaaga caccgctgcg cgcctgcaaa gcaggggtat tagcgcggcg gcctatcatg
     5941 ccgggctgga aaataatgtc cgcgccgacg tgcaggagaa attccagcgc gatgatctgc
     6001 aaattgtggt ggcgacggtg gcgttcggca tgggcatcaa taaaccaaac gttcgcttcg
     6061 tggtccactt tgatattccg cgcaatatcg aatcctatta tcaggaaacc ggtcgcgccg
     6121 ggcgtgatgg cctgcctgcg gaagcgatgc tgttttacga tccggctgat atggcgtggc
     6181 tgcgccgttg tctggaagag aagccgcagg ggcagttgca ggatatcgag cgccacaaac
     6241 tcaatgcgat gggcgcgttt gccgaagcgc aaacttgccg tcgtctggta ttgctgaact
     6301 attttggcga agggcgtcag gagccgtgcg ggaactgcga tatctgcctc gatccgccga
     6361 aacagtacga cggttcaacc gatgctcaga ttgccctttc caccattggt cgtgtgaatc
     6421 agcggtttgg gatgggttat gtggtggaag tgattcgtgg tgctaataac cagcgtatcc
     6481 gcgactatgg tcatgacaaa ctgaaagtct atggtatggg ccgtgataaa agccatgaac
     6541 attgggtgag cgtgatccgc cagctgattc atctcggcct ggtgacgcaa aatattgccc
     6601 agcattctgc cctacaactg acagaggccg cgcgcccggt gctgcgcggc gaatcctctt
     6661 tgcaacttgc cgtgccgcgt atcgtggcgc tcaaaccgaa agcgatgcag aaatcgttcg
     6721 gcggcaacta tgatcgcaaa ctgttcgcca aattacgcaa actgcgtaaa tcgattgccg
     6781 atgaaagtaa tgtcccgccg tacgtggtgt ttaacgacgc aaccttgatt gagatggctg
     6841 aacagatgcc gatcaccgcc agcgaaatgc tcagcgttaa cggcgttggg atgcgcaagc
     6901 tggaacgctt tggtaaaccg tttatggcgc tgattcgtgc gcatgtcgat ggcgacgacg
     6961 aagagtagtc agcagcataa aaaagtgcca gtatgaagac tccgtaaacg tttcccccgc
     7021 gagtcaaatg tatgttgatg ttatttctca ccgtcgccat ggtgcacatt gtggcgctta
     7081 tgagccccgg tcccgatttc ttttttgtct ctcagaccgc tgtcagtcgt tcccgtaaag
     7141 aagcgatgat gggcgtgctg ggcattacct gcggcgtaat ggtttgggct gggattgcgt
     7201 tgcttggcct gcatttgatt atcgaaaaaa tggcctggct gcatacgctg attatggtag
     7261 gcggtggcct gtatctctgc tggatgggtt accagatgct acgcggtgca ctgaaaaaag
     7321 aggctgtatc tgcgcctgca ccacaggttg agctggcgaa aagcgggcgc agtttcctga
     7381 aaggcttact gaccaatctc gccaatccga aagcgattat ctattttggt tcggtattct
     7441 cattgtttgt cggcgataac gttggcacta ccgagcgctg gggcattttc gcgttgatta
     7501 ttgtcgaaac actggcgtgg tttaccgtcg ttgccagtct gtttgccctg ccgcaaatgc
     7561 gccgtggtta tcaacgtctg gcgaagtgga ttgatggttt tgccggggcg ttatttgccg
     7621 gatttggcat tcatttgatt atttcgcggt gatgccagac gcgtcttcag agtaagtcgg
     7681 ataaggcgtt tacgccgcat ccgacattat ttttcacgca tgcctcgccg atgctaacag
     7741 cgctcccacc agcataaaca acgagccgaa aatcttattc agcgccttca tctgctttgg
     7801 tcctttaatc catagagcaa tcctttgagc aagggtggcg taaccgatca tcacaataat
     7861 atcgaccaca atagtggtga cgccgagcac gatatactgc atcagttgcg gctgttgcgg
     7921 catgatgaat tgcggaaata gcgccgccag aaacacaata cttttgggat tggtgagatt
     7981 cacaaaaact gcgcgctgga acaaatgtcg acgcgattga gtagaggcca gcgatttaag
     8041 atcaattgcg ccagcggcgc gccactgctg gattcccagc caaatcaagt aagccgcgcc
     8101 tgcccacttt aacacttcaa acgcaatcac tgagcgggaa aatagcgtcc ccaaccccac
     8161 gccaaccagc acaatatgaa tcgccagtcc ggtctgaagc ccagcaatag acgccaccgc
     8221 gccgcgataa ccgtggttga gcgaggtggt catagtgttg attgcaccag agcctggcga
     8281 caggcttaaa atgatcgatg tcagcaggta ggcaaaccac cattctaagg tcatgatgaa
     8341 ctcccggtgt gtctattttt gtgccacaat acgctactgt cgcagcgttg tgtcaggcac
     8401 gcttaaaaaa acgattttac gtggtttaag aggcagatta cccgatgttt cagcagcaaa
     8461 aagactggga aacaagagaa aacgcgtttg ctgcttttac catgggaccg ctgactgatt
     8521 tctggcgtca gcgtgatgaa gcagagttta ctggtgtgga tgacattccg gtgcgctttg
     8581 tccgttttcg cgcacagcac catgaccggg tggtagtcat ctgcccgggg cgtattgaga
     8641 gctacgtaaa atatgcggaa ctggcctacg acctgttcca tttggggttt gatgtcttaa
     8701 tcatcgacca tcgcgggcag ggacgttccg gtcgcctgtt agccgatccg catctcgggc
     8761 atgttaatcg ctttaatgat tatgttgatg atctggcggc attctggcag caggaggttc
     8821 agcccggtcc gtggcgtaaa cgctatatac tggcacattc gatgggcggt gcgatctcca
     8881 cattatttct gcaacgccat ccaggtgtat gtgacgccat tgcgctaact gcgccaatgt
     8941 ttgggatcgt gattcgtatg ccgtcattta tggcacggca gatcctcaac tgggccgaag
     9001 cgcatccacg tttccgtgat ggctatgcaa taggcaccgg gcgctggcgc gcgttgccgt
     9061 ttgctatcaa cgtactgacc cacagcagac agcgatatcg acgtaactta cgcttctatg
     9121 ctgatgaccc aacgattcgc gtcggtgggc cgacctacca ttgggtacgc gaaagtattc
     9181 tggctggcga acaggtgtta gccggtgcgg gtgatgacgc cacgcaaacg cttctcttac
     9241 aagctgaaga ggaacgcgtg gtggataacc gcatgcacga ccgtttttgt gaactccgca
     9301 ccgccgcggg ccatcctgtc gaaggaggac ggccgttggt aataaaaggt gcttaccatg
     9361 agatcctttt tgaaaaggac gcaatgcgct cagtcgcgct ccacgccatc gttgattttt
     9421 tcaacaggca taactcaccc agcggaaacc gctctacaga ggtttaaatt tcttatgtac
     9481 caggttgttg cgtctgattt agatggcacg ttactttctc ccgaccatac gttatcccct
     9541 tacgccaaag aaactctgaa gctgctcacc gcgcgcggca tcaactttgt gtttgcgacc
     9601 ggtcgtcacc acgttgatgt ggggcaaatt cgcgataatc tggagattaa gtcttacatg
     9661 attacctcca atggtgcgcg cgttcacgat ctggatggca atctgatttt tgctcataac
     9721 ctggatcgcg acattgccag cgatctgttt ggcgtagtca acgacaatcc ggacatcatt
     9781 actaacgttt atcgcgacga cgaatggttt atgaatcgcc atcgcccgga agagatgcgc
     9841 ttttttaaag aagcggtgtt caaatatgcg ctgtatgagc cgggattact ggagccggaa
     9901 ggcatcagca aagtgttttt cacctgcgat tcccatgaac aactgctgcc gctggagcag
     9961 gcgattaacg ctcgctgggg cgatcgcgtc aacgtcagtt tctctacctt aacctgtctg
    10021 gaagtgatgg cgggcggcgt gtcgaaaggc catgcgctgg aagcagtggc gaagaaactg
    10081 ggctacagcc tgaaggattg tattgcgttt ggtgacggga tgaacgacgc cgaaatgctg
    10141 tcgatggcgg ggaaaggctg cattatgggc agtgcgcacc agcgtctgaa agatctgcat
    10201 ccggagctgg aagttattgg tactaatgcc gacgacgcgg tgccgcatta tctgcgtaaa
    10261 ctctatttat cgtaatcgtt ctttatttgg tcagttgtca acctgatact tcgctacaat
    10321 ggatactcgt taatcaaaga gttttccatt gtggcgttac ttatcatcac cacgattctg
    10381 tgggccttct cctttagctt ttatggcgag taccttgcgg ggcacgtcga tagctatttt
    10441 gcggtgctgg tgcgcgttgg cttggcggca ctcgtttttc tgccgtttct gcgtacccgt
    10501 agcaatagcc tgaaaacggt cggcctgtat atgctggtgg gcgcgatgca gctcggcgtg
    10561 atgtatatgc tgagtttccg cgcttatctc tacctgacgg tctccgaatt gttgctgttc
    10621 accgtactga cgccgctcta catcacgctg atttatgaca tcatgagtaa gcgccgtctg
    10681 cgctggggct atgcttttag cgccttgctg gcggtgattg gtgccgggat tattcgctat
    10741 gatcaggtca ccgaccattt ctggactggc ttgttgctgg tgcaactctc caatatcact
    10801 tttgccatcg gcatggtggg ttacaaacgc ctgatggaaa ctcgcccgat gccgcagtat
    10861 aacgcctttg cgtggttcta tcttggcgcg ttcctggtgg cggtaattgc atggttcttg
    10921 ctgggaaatg cgcagaaaat gccgcaaacc acgttgcaat ggggcattct ggtgtttctt
    10981 ggcgtggtgg cttccgggat tggctacttt atgtggaact acggcgcgac gcaggtggac
    11041 gccggaacgc tgggcattat gaataatatg cacgttccgg cagggctgct ggtaaacctg
    11101 gctatctggc accaacagcc gcactggcca acgtttatta caggcgcgct ggtgatcctg
    11161 gcctcactgt gggtgcatcg taagtgggtc gctccgcgct cttcacaaac ggcagatgat
    11221 cgcaggcgtg attgcgcgct gagcgaataa acgcttccgt aactggctga cgctgctcgc
    11281 catcgcgcac ggcggcgtac agtcggctcc acaagccttc gcccagggtt ttggtcacca
    11341 ccagaccctg gcgctcaaaa ctctctacta cccaatgcgg tagcgcggca atacccatcc
    11401 gcgcggcaac catttgaatc aacaataagg tgttatcgac gcttttcagt gacgggctga
    11461 cgcctgccgg ctgaagaaaa tgccgccaga catccagtcg actacgctgc accggataaa
    11521 ttaatagcgt ctcgctggcg agatcttccg gtgtaatacg cgtttttgcc gccagtggat
    11581 ggtcaggtgc taacaccagg cgtacttcat aatcgaacat cggcgaataa tgcaggccac
    11641 tgcgcggcag gatatcggag gtcatcacca gatccagctc gccctgttgc aacgcgggct
    11701 gcgggtcaaa cgtcacgccc gatttaaaat ccatctctac ctgcggccag ttcttatgga
    11761 aatttcctaa agcgggtgtc agccactgaa tacagctatg gcactcaatg gcaatgcgca
    11821 gacgcgtctg ctgcggttca ttgcaggctt gcagtgcctg gctgatttgc ggcagcacct
    11881 ggtttgccag ttgcaacagg atttctccct gcggtgtaaa gcgtagcggc tggctcttac
    11941 gcacaaatag ccggaagcca aggcgttgtt ccagatcgct aaactggtga gacagggcgg
    12001 attgcgtctg atgcaacgtt gccgcagcgg ctgcgagcga gccgcagttc cgcaacgctt
    12061 gtagcgtttt caggtgtttt acttcgatca tgaaagtcct tcacttcggc atgaataatt
    12121 tgcgcttgag gaatatacag taaccgccaa ttatggatgt gtaaacatct ggacggctaa
    12181 aatcattcgt cttttaaatt tatggtgcgt tggctgcgtt tctccacccc ggtcacttac
    12241 ttcagtaagc tcccggcgat gaataaactt gccgccttcc ctaaattcaa aatccatagg
    12301 atttacatat aattagagga agaaaaatga caatattgaa tcacaccctc ggtttccctc
    12361 gcgttggcct gcgtcgcgag ctgaaaaaag cgcaagaaag ttattgggcg gggaactcca
    12421 cgcgtgaaga actgctgaca gtagggcgtg aactgcgtgc tcgtcactgg gatcaacaaa
    12481 agcaagcggg tatcgacctg ctgccggtgg gcgattttgc ctggtacgat catgtactga
    12541 ccaccagttt gctgctgggt aatgttccgc cacgtcatca gaacaaagac ggttcggtag
    12601 atatcgacac cctgttccgt attggtcgtg gacgtgcgcc gactggcgaa cctgcggcgg
    12661 cagcggaaat gaccaaatgg tttaacacca actatcacta catggtgccg gagttcgtta
    12721 aaggccaaca gttcaaactg acttggacgc agctgctgga ggaagtggac gaggcgctgg
    12781 cgctgagcca caacgttaag cctgtgctgc tggggccggt tacttacctg tggctgggga
    12841 aagtgaaagg tgaacagttt gaccgcctta gcctgctgaa cgacattctg ccggtttatc
    12901 agcaagtgct ggcagaactg gcgaaacgcg gcatcgagtg ggtacagatt gatgaacccg
    12961 cgctggtact ggaactacca caggcgtggc tggacgcata caaacccgct tacgacgcgc
    13021 tccagggaca ggtgaaactg ctgctgacca cctattttga aggcgtaacg ccaaatctcg
    13081 acacgattac tgcgctgcct gttcaggggc tgcatgtgga ccttgtacat ggtaaagata
    13141 acgttgttga actgcacaag cgcctgcctt ctgactggct gctgtctgcg ggtctgatca
    13201 atggtcgtaa cgtctggcgc gccgatctta ctgagaaata tgcgcaaatt aaggacattg
    13261 tcggcaaacg tgatttgtgg gtggcatctt cctgctcgtt gctgcacagc cccatcgacc
    13321 tgagcgtgga aacgcgtctt gatgcagaag tgaaaagctg gtttgccttc gccctacaaa
    13381 aatgccatga actggcactg ctgcgcgatg cgctgaacag tggtgacacg gcagctctgg
    13441 cagagtggag cgccccaatt caggcgcgtc gtcactctac tcgtgtacat aatccggcgg
    13501 tagaaaagcg tctggcggcg atcaccgccc aggacagcca gcgtgcgaat gtctatgaag
    13561 tgcgtgctga agcccagcgt gcgcgtttta aactgccagc gtggccgacc accacgattg
    13621 gttccttccc gcaaaccacg gaaattcgta ccctgcgtct ggatttcaaa aagggcaatc
    13681 tcgacgccaa caactaccgc acgggcattg cggaacatat caagcaggcc attgttgagc
    13741 aggaacgttt gggactggat gtgctggtac atggcgaggc cgagcgtaat gacatggtgg
    13801 aatactttgg cgagcacctc gacggatttg tctttacgca aaacggttgg gtacagagct
    13861 acggttcccg ctgcgtgaag ccaccgattg tcattggtga cattagccgc ccggcaccga
    13921 ttaccgtgga gtgggcgaag tatgcgcaat cgctgaccga caaaccggtg aaagggatgc
    13981 tgacggggcc ggtgaccata ctctgctggt cgttcccgcg tgaagatgtc agccgtgaaa
    14041 ccatcgccaa acagattgcg ctggcgctgc gtgatgaagt ggccgatctg gaagccgctg
    14101 gaattggcat catccagatt gacgaaccgg cgctgcgcga aggtttaccg ctgcgtcgta
    14161 gcgactggga tgcgtatctc cagtggggcg tagaggcctt ccgtatcaac gccgccgtgg
    14221 cgaaagatga cacacaaatc cacactcaca tgtgttattg cgagttcaac gacatcatgg
    14281 attcgattgc ggcgctggac gcagacgtca tcaccatcga aacctcgcgt tccgacatgg
    14341 agttgctgga gtcgtttgaa gagtttgatt atccaaatga aatcggtcct ggcgtctatg
    14401 acattcactc gccaaacgta ccgagcgtgg aatggattga agccttgctg aagaaagcgg
    14461 caaaacgcat tccggcagag cgcctgtggg tcaacccgga ctgtggcctg aaaacgcgcg
    14521 gctggccaga aacccgcgcg gcactggcga acatggtgca ggcggcgcag aatttgcgtc
    14581 gggggtaaat ccaaaccggg tggtaatacc acccggtctt ttctcattac agcgacttct
    14641 tcccaccata ctgcttaaac cattccagca tacgctgcca gccatctttc gcagattcgg
    14701 catgatagct cgggcgataa tcagcgttga atgcatgccc ggcatccggg tacacgataa
    14761 tctctgcttt cgcattagca gcccgcagcg cctggcgcat cgtttcaacg ctctcctgcg
    14821 gaatgctgtt atcctgacca ccatataagc cgagaaccgg cgcgttaaga tcggttgcga
    14881 tatcaacagg ttgtttcggt gaattcagcg acttgtcgcc cgtcagtttg ccgtaccacg
    14941 ccactgcggc ttttagctgt ggattatgcg cggcatacag ccaggtgata cgtccacccc
    15001 agcagaatcc ggtgatcatt aaacgatgaa catcgccgcc gttgcgggaa gcccaactgg
    15061 cgacatgatc gagatcggcc agcacctgcg agtcaggcac ttttgctacc agaccgctaa
    15121 gcaacgtggg gatatcggca aaatcattcg gatcgccttc gcggaagtaa agttcaggtg
    15181 cgatagccag atacccctcc agcgccagac ggcgacaaat gtcgcggata tgttcatgca
    15241 cgccaaaaat ttcctgcact acaatgacca ctggcagtgg gccatcgctt tgctttggtc
    15301 tggcatggta agcaggcatg ttatcccctt gtgaagggat agaggtgaag cccgccacaa
    15361 tcgcgtcgtc cggggtctga acgatggtcg aagcgagagg cgatgcagca ggtgcaaatc
    15421 cagattgttg tgttgttgcc atggtattct ccgtaccctt ataaaaatgt tgcgcaatgt
    15481 taactatagt cagcatgcaa caaatcacat tgcctgaatc ggctcatctt ttatgcagtc
    15541 ctgcagaatg aagagtgatt tatgtgatat gaatcacttt tggtgggtaa atttatgcaa
    15601 cgcatttgcg tcatggtgat gagtatcacg aaaaaatgtt aaacccttcg gtaaagtgtc
    15661 tttttgcttc ttctgactaa accgattcac agaggagttg tatatgtcca agtctgatgt
    15721 ttttcatctc ggcctcacta aaaacgattt acaaggggct acgcttgcca tcgtccctgg
    15781 cgacccggat cgtgtggaaa agatcgccgc gctgatggat aagccggtta agctggcatc
    15841 tcaccgcgaa ttcactacct ggcgtgcaga gctggatggt aaacctgtta tcgtctgctc
    15901 taccggtatc ggcggcccgt ctacctctat tgctgttgaa gagctggcac agctgggcat
    15961 tcgcaccttc ctgcgtatcg gtacaacggg cgctattcag ccgcatatta atgtgggtga
    16021 tgtcctggtt accacggcgt ctgtccgtct ggatggcgcg agcctgcact tcgcaccgct
    16081 ggaattcccg gctgtcgctg atttcgaatg tacgactgcg ctggttgaag ctgcgaaatc
    16141 tattggcgcg acaactcacg ttggcgtgac agtttcttct gataccttct acccaggtca
    16201 ggaacgttac gatacttact ctggtcgcgt agttcgtcac tttaaaggtt ctatggaaga
    16261 gtggcaggcg atgggcgtaa tgaactatga aatggaatct gcaactctgc tgaccatgtg
    16321 tgcaagtcag ggcctgcgtg ccggtatggt agcgggtgtt atcgttaacc gcacccagca
    16381 agagatcccg aatgctgaga cgatgaaaca aaccgaaagc catgcggtga aaatcgtggt
    16441 ggaagcggcg cgtcgtctgc tgtaattctc ttctcctgtc tgaaggccga cgcgttcggc
    16501 cttttgtatt tttgcgtagc gcctcgcagg aaatgccttt ccaactggac gtttgtacag
    16561 cacaattcta ttttgtgcgg gtaagttgtt gcgtcaggag gcgttgtgga tttctcaatc
    16621 atggtttacg cagttattgc gttggtgggt gtggcaattg gctggctgtt tgccagttat
    16681 caacatgcgc agcaaaaagc cgagcaatta gctgaacgtg aagagatggt cgcggagtta
    16741 agcgcggcaa aacaacaaat tacccaaagc gagcactggc gtgcagagtg tgaattgctc
    16801 aataacgaag tgcgcagcct gcaaagtatt aacacctctc tggaggccga tctgcgtgaa
    16861 gtaaccacgc ggatggaagc cgcacagcaa catgctgacg ataaaattcg ccagatgatt
    16921 aacagcgagc agcgcctcag tgagcagttt gaaaacctcg ccaaccgtat ttttgagcac
    16981 agcaatcgcc gggttgatga gcaaaaccgt cagagtctga acagcctgtt gtcgccgcta
    17041 cgtgaacaac tggacggttt ccgccgtcag gttcaggaca gcttcggtaa agaagcacaa
    17101 gaacgccata ccctgaccca cgaaattcgc aatctccagc aactcaacgc gcaaatggcc
    17161 caggaagcga tcaacctgac gcgcgcgctg aaaggcgaca ataaaaccca gggcaactgg
    17221 ggcgaggtag tattgacgcg ggtgctggag gcttccggtc tgcgtgaagg gtatgaatat
    17281 gaaacccagg tcagcatcga aaatgacgcc cgctcgcgga tgcagccgga tgtcatcgtg
    17341 cggctgccgc agggaaaaga tgtggtgatc gacgccaaaa tgacgctggt cgcctatgaa
    17401 cgctatttta acgccgaaga cgactacacc cgcgaaagcg cgctacagga acatatcgcg
    17461 tcggtgcgta accatatccg tttgctggga cgcaaagatt atcaacagct gccggggctg
    17521 cgaactctgg attacgtgct gatgtttatt cccgttgaac ccgctttttt actggcgctt
    17581 gaccgccagc cggagctgat caccgaagcg ttgaaaaaca atatcatgct ggttagcccg
    17641 actacgctgc tggtggcgtt gcgtactatt gccaacctgt ggcgttatga gcatcaaagc
    17701 cgtaacgccc agcaaatcgc cgatcgtgcc agcaagctgt acgacaagat gcgtttgttc
    17761 atcgatgaca tgtccgcgat tggtcaaagt ctcgacaaag cgcaggataa ctaccgccag
    17821 gcaatgaaaa aactctcttc aggtcgcgga aatgtgctgg cgcaggcaga agcgtttcgc
    17881 ggtttaggag tagaaattaa acgcgagatt aatccggatt tggctgaaca ggcggtgagc
    17941 caggatgaag agtatcgact tcggtcggtt ccggagcagc cgaatgatga agcttatcaa
    18001 cgcgatgatg aatataatca gcagtcgcgc tagcccatta ggggtagtta agccgggtag
    18061 aaatctaggg catcgacgcc caatctgtta cacttctgga acaatttttt gatgagcagg
    18121 cattgagatg gtggataagt cacaagaaac gacgcacttt ggttttcaga ccgtcgcgaa
    18181 ggaacaaaaa gcggatatgg tcgcccacgt tttccattcc gtggcatcaa aatacgatgt
    18241 catgaatgat ttgatgtcat ttggtattca tcgtttgtgg aagcgattca cgattgattg
    18301 cagcggcgta cgccgtgggc agaccgtgct ggatctggct ggtggcaccg gcgacctgac
    18361 agcgaaattc tcccgcctgg tcggagaaac tggcaaagtg gtccttgctg atatcaatga
    18421 atccatgctc aaaatgggac gcgagaagct gcgtaatatc ggtgtgattg gcaacgttga
    18481 gtatgttcag gcgaacgctg aggcgctgcc gttcccggat aacacctttg attgcatcac
    18541 catttcgttt ggtctgcgta acgtcaccga caaagataaa gcactgcgtt caatgtatcg
    18601 cgtgctgaaa cccggcggcc gcctgctggt gcttgagttc tcgaagccaa ttatcgagcc
    18661 gctgagcaaa gcctatgatg catactcctt ccatgtgctg ccgcgtattg gctcactggt
    18721 cgcgaacgac gccgacagct accgttatct ggcagaatcc atccgtatgc atcccgatca
    18781 ggataccctg aaagccatga tgcaggatgc cggattcgaa agtgtcgact actacaatct
    18841 gacggcaggg gttgtggcgc tgcatcgtgg ttataagttc tgacaggaga ccggaaatgc
    18901 cttttaaacc tttagtgacg gcaggaattg aaagtctgct caacaccttc ctgtatcgct
    18961 cacccgcgct gaaaacagcc cgctcgcgtc tgctgggtaa agtattgcgc gtggaggtaa
    19021 aaggcttttc gacgtcattg attctggtgt tcagcgaacg ccaggttgat gtactgggcg
    19081 aatgggcagg cgatgctgac tgcaccgtta tcgcctacgc cagtgtgttg ccgaaacttc
    19141 gcgatcgcca gcagcttacc gcactgattc gcagtggtga gctggaagtg cagggcgata
    19201 ttcaggtggt gcaaaacttc gttgcgctgg cagatctggc agagttcgac cctgcggaac
    19261 tgctggcccc ttataccggt gatatcgccg ctgaaggaat cagcaaagcc atgcgcggag
    19321 gcgcaaagtt cctgcatcac ggcattaagc gccagcaacg ttatgtggcg gaagccatta
    19381 ctgaagagtg gcgtatggca cccggtccgc ttgaagtggc ctggtttgcg gaagagacgg
    19441 ctgccgtcga gcgtgctgtt gatgccctga ccaaacggct ggaaaaactg gaggctaaat
    19501 gacgccaggt gaagtacggc gcctatattt catcattcgc acttttttaa gctacggact
    19561 tgatgaactg atccccaaaa tgcgtatcac cctgccgcta cggctatggc gatactcatt
    19621 attctggatg ccaaatcggc ataaagacaa acttttaggt gagcgactac gactggccct
    19681 gcaagaactg gggccggttt ggatcaagtt cgggcaaatg ttatcaaccc gccgcgatct
    19741 ttttccaccg catattgccg atcagctggc gttattgcag gacaaagttg ctccgtttga
    19801 tggcaagctg gcgaagcagc agattgaagc tgcaatgggc ggcttgccgg tagaagcgtg
    19861 gtttgacgat tttgaaatca agccgctggc ttctgcttct atcgcccagg ttcataccgc
    19921 gcgattgaaa tcgaatggta aagaggtggt gattaaagtc atccgcccgg atattttgcc
    19981 ggtcattaaa gcggatctga aacttatcta ccgtctggct cgctgggtgc cgcgtttgct
    20041 gccggatggt cgccgtctgc gcccaaccga agtggtgcgc gagtacgaaa agaccttgat
    20101 tgatgaactg aatttgctgc gggaatctgc caacgccatt cagcttcggc gcaattttga
    20161 agacagcccg atgctctaca tcccggaagt ttaccctgac tattgtagtg aagggatgat
    20221 ggtgatggag cgcatttacg gcattccggt gtctgatgtt gcggcgctgg agaaaaacgg
    20281 cactaacatg aaattgctgg cggaacgcgg cgtgcaggtg ttcttcactc aggtctttcg
    20341 cgacagcttt ttccatgccg atatgcaccc tggcaacatc ttcgtaagct atgaacaccc
    20401 ggaaaacccg aaatatatcg gcattgattg cgggattgtt ggctcgctaa acaaagaaga
    20461 taaacgctat ctggcagaaa actttatcgc cttctttaat cgcgactatc gcaaagtggc
    20521 agagctacac gtcgattctg gctgggtgcc accagatacc aacgttgaag agttcgaatt
    20581 tgccattcgt acggtctgtg aacctatctt tgagaaaccg ctggccgaaa tttcgtttgg
    20641 acatgtactg ttaaatctgt ttaatacggc gcgtcgcttc aatatggaag tgcagccgca
    20701 actggtgtta ctccagaaaa ccctgctcta tgtcgaaggg gtaggacgcc agctttatcc
    20761 gcagctcgat ttatggaaaa cagcgaagcc tttcctggag tcgtggatta aagatcaggt
    20821 cggtattcct gctctggtga gagcatttaa agaaaaagcg ccgttctggg tcgaaaaaat
    20881 gccagaactg cctgaactgg tttacgacag tttgcgccag ggcaagtatt tacagcacag
    20941 tgttgataag attgcccgcg agcttcagtc aaatcatgta cgtcagggac aatcgcgtta
    21001 ttttctcgga attggcgcta cgttagtatt aagtggcaca ttcttgttgg tcagccgacc
    21061 tgagtggggg ctgatgcccg gctggttaat ggcaggtggt ctgatcgcct ggtttgtcgg
    21121 ttggcgcaaa acacgctgat tttttcatcg ctcaaggcgg gccgtgtaac gtataatgcg
    21181 gctttgttta atcatcatct accacagagg aacatgtatg ggtggtatca gtatttggca
    21241 gttattgatt attgccgtca tcgttgtact gctttttggc accaaaaagc tcggctccat
    21301 cggttccgat cttggtgcgt cgatcaaagg ctttaaaaaa gcaatgagcg atgatgaacc
    21361 aaagcaggat aaaaccagtc aggatgctga ttttactgcg aaaactatcg ccgataagca
    21421 ggcggatacg aatcaggaac aggctaaaac agaagacgcg aagcgccacg ataaagagca
    21481 ggtgtaatcc gtgtttgata tcggttttag cgaactgcta ttggtgttca tcatcggcct
    21541 cgtcgttctg gggccgcaac gactgcctgt ggcggtaaaa acggtagcgg gctggattcg
    21601 cgcgttgcgt tcactggcga caacggtgca gaacgaactg acccaggagt taaaactcca
    21661 ggagtttcag gacagtctga aaaaggttga aaaggcgagc ctcactaacc tgacgcccga
    21721 actgaaagcg tcgatggatg aactacgcca ggccgcggag tcgatgaagc gttcctacgt
    21781 tgcaaacgat cctgaaaagg cgagcgatga agcgcacacc atccataacc cggtggtgaa
    21841 agataatgaa gctgcgcatg agggcgtaac gcctgccgct gcacaaacgc aggccagttc
    21901 gccggaacag aagccagaaa ccacgccaga gccggtggta aaacctgctg cggacgctga
    21961 accgaaaacc gctgcacctt ccccttcgtc gagtgataaa ccgtaaacat gtctgtagaa
    22021 gatactcaac cgcttatcac gcatctgatt gagctgcgta agcgtctgct gaactgcatt
    22081 atcgcggtga tcgtgatatt cctgtgtctg gtctatttcg ccaatgacat ctatcacttg
    22141 gtatccgcgc cattgatcaa gcagttgccg caaggttcaa cgatgatcgc caccgacgtg
    22201 gcctcgccgt tctttacgcc gatcaagctg acctttatgg tgtcgctgat tctgtcagcg
    22261 ccggtgattc tctatcaggt gtgggcattt atcgccccag cgctgtataa gcatgaacgt
    22321 cgcctggtgg tgccgctgct ggtttccagc tctctgctgt tttatatcgg catggcattc
    22381 gcctactttg tggtctttcc gctggcattt ggtttccttg ccaataccgc gccggaaggg
    22441 gtgcaggtat ccaccgacat cgccagctat ttaagcttcg ttatggcgct gtttatggcg
    22501 tttggtgtct cctttgaagt gccggtggca attgtgctgc tgtgctggat ggggattacc
    22561 tcgtcagaag acttacgcaa aaaacgcccg tatgtgctgg ttggtgcatt cgttgtcggg
    22621 atgttgctga cgccgccgga tgtcttctcg caaacgctat tggcgatccc gatgtactgt
    22681 ctgtttgaaa tcggtgtctt cttctcacgc ttttacgttg gtaaagggcg aaatcgggaa
    22741 gaggaaaacg acgctgaagc agaaagcgaa aaaactgaag aataaattca accgcccgtc
    22801 agggcggttg tcatatggag tacaggatgt ttgatatcgg cgttaatttg accagttcgc
    22861 aatttgcgaa agaccgtgat gatgttgtag cgcgcgcttt tgacgcggga gttaatgggc
    22921 tactcatcac cggcactaac ctgcgtgaaa gccagcaggc gcaaaagctg gcgcgtcagt
    22981 attcgtcctg ttggtcaacg gcgggcgtac atcctcacga cagcagccag tggcaagctg
    23041 cgactgaaga agcgattatt gagctggccg cgcagccaga agtggtggcg attggtgaat
    23101 gtggtctcga ctttaaccgt aacttttcga cgccggaaga gcaggaacgc gcttttgttg
    23161 ctcagctacg cattgccgca gaattaaaca tgccggtatt tatgcactgt cgcgatgccc
    23221 acgagcggtt tatgacattg ctggagccgt ggctggataa actgcctggt gcggttcttc
    23281 attgctttac cggcacacgc gaagagatgc aggcgtgcgt ggcgcgtgga atttatatcg
    23341 gcattaccgg ttgggtttgc gatgaacgac gcgggctgga gctgcgggaa ttgttgccgt
    23401 tgattccggc ggagaaattg ctgatcgaaa cagatgcgcc gtatctgctc ccgcgcgatc
    23461 tcacgccaaa gccatcatcc cggcgcaacg agccagccca tctgccccat attttgcaac
    23521 gtattgcgca ctggcgtgga gaagatgccg catggctggc tgccaccacg gatgccaatg
    23581 tcaaaacact gtttgggatt gcgttttaga gtttgcggaa ctcggtgttc ttcacactgt
    23641 gcttaatctc tttattaata agattaagca atagcatgga gcgagcctca ccatcgggtt
    23701 cggtgaaaat ggcctgaaag ccttcgaacg cgccttcggt aataatcacc ttatcacccg
    23761 gataaggggg ggccggatcg acaatgtctt tcggtttata taccgatagc tgatgaatta
    23821 ccgccgatgg gactatcgct ggcgacgcgc caaagcgcac aaagtggctg acgccacgcg
    23881 tagcgctgat ggtcgtggta tgaatcactt ctggatcaaa ttccacaaac aggtagttag
    23941 ggaataacgg ttcactgact gcggtacgtt taccccgtac gattttttcc agggtgatca
    24001 tcggagcgag gcaattaacc gcctgtctct caaggtgttc ctgggcacgt tgaagttgcc
    24061 cgcgcttgca gtacagtaaa taccaggatt gcataatgac tcttatccgt ttaatcgggg
    24121 cgcaaggata gcaaaagctt tacgctaagt taattatatt ccccggtttg cgttataccg
    24181 tcagagttca cgctaattta acaaatttac agcattgcaa agatgaacgc cgtataatgg
    24241 gcgcagatta agaggctaca atggacgcca tgaaatataa cgatttacgc gacttcctga
    24301 cgctgcttga acagcagggt gagctaaaac gtatcacgct cccggtggat ccgcatctgg
    24361 aaatcactga aattgctgac cgcactctgc gtgctggtgg gcctgcgctg ttgttcgaaa
    24421 accctaaagg ctactcaatg ccggtgctgt gcaacctgtt cggtacgcca aagcgcgtgg
    24481 cgatgggcat ggggcaggaa gatgtttcgg cgctgcgtga agttggtaaa ttattggcgt
    24541 ttctgaaaga gccggagccg ccaaaaggtt tccgcgacct gtttgataaa ctgccgcagt
    24601 ttaagcaggt gttaaacatg ccgacaaagc gactgcgtgg tgcgccctgc caacaaaaaa
    24661 tcgtctctgg cgatgacgtc gatctcaatc gcattcccat tatgacctgc tggccggaag
    24721 atgccgcgcc gctgattacc tgggggctga ccgtaacgcg cggcccgcat aaagagcggc
    24781 agaatctggg catttatcgc cagcagctga ttggtaaaaa caaactgatt atgcgctggc
    24841 tgtcgcatcg cggcggcgcg ctggattatc aggagtggtg tgcggcgcat ccgggcgaac
    24901 gtttcccggt ttctgtggcg ctgggtgccg atcctgccac gattctcggt gcagtcaccc
    24961 ccgttccgga tacgctttca gagtatgcgt ttgccggatt gctacgcggc accaaaaccg
    25021 aagtagtaaa gtgtatttcc aatgacctcg aagtgccagc cagtgccgaa atcgtcctgg
    25081 aagggtatat cgatcctggt gagatggcgc cggaagggcc gtatggtgac cacacaggtt
    25141 actataatga agtcgataat ttcccggtgt ttaccgtgac gcatattacc cagcgtgaag
    25201 atgcgattta ccattccacc tataccgggc gtccgccaga tgagcccgcg gtactgggcg
    25261 tggcgttgaa cgaagtgttt gtaccgattc tgcaaaaaca gttcccggaa attgtcgatt
    25321 tttacctgcc gccggaaggc tgttcttatc gtctggcggt agtgacgatc aaaaaacagt
    25381 acgccggaca cgcgaagcgc gtcatgatgg gcgtctggtc gttcttacgc cagtttatgt
    25441 acactaaatt tgtgatcgtt tgcgatgatg acgtcaacgc acgcgactgg aacgatgtga
    25501 tttgggcgat taccacccgt atggacccgg cgcgggatac tgttctggta gaaaatacgc
    25561 ctattgatta tctggatttt gcctcgcctg tctctgggct gggttcaaaa atggggctgg
    25621 atgccacgaa taaatggccg ggggaaaccc agcgtgaatg gggacgtccc atcaaaaaag
    25681 atccagatgt tgtcgcgcat attgacgcca tctgggatga actggctatt tttaacaacg
    25741 gtaaaagcgc ctgatgcgcg tttgttttgc cctatttatc gatccgacag agaaagcgca
    25801 tgacaacctt aagctgtaaa gtgacctcgg tagcagctat cacggatacc gtatatcgtg
    25861 tccgcatcgt gccagacgcg gccttttctt ttcgtgctgg tcagtatttg atggtagtga
    25921 tggatgagcg cgacaaacgt ccgttctcaa tggcctcgac gccggatgaa aaagggttta
    25981 tcgagctgca tattggcgct tctgaaatca acctttacgc gaaagcagtc atggaccgca
    26041 tcctcaaaga tcatcaaatc gtggtcgacc ttccccacgg agaagcgtgg ctgcgcgatg
    26101 atgaagagcg tccgatgatt ttgattgcgg gcggcaccgg gttctcttat gcccgctcga
    26161 ttttgctgac agcgttggcg cgtaacccaa accgtgatat caccatttac tggggcgggc
    26221 gtgaagagca gcatctgtat gatctctgcg agcttgaggc gctttcgtta aagcaccttg
    26281 gtctgcaagt ggtgccggtg gttgaacaac cggaagcagg ctggcgtggg cgtactggta
    26341 ccgtgttaac ggcggtattg caggatcatg gtacgctggc agagcatgat atctatattg
    26401 ccggacgttt tgagatggcg aaaattgccc gtgacctgtt ttgcagtgag cgtaatgcgc
    26461 gggaagatcg cctgtttggc gatgcgtttg catttatctg agatataaaa aaacccgccc
    26521 ctgacaggcg ggaagaacgg caactaaaac tttattcagt ggcatttaga tctatgtcgc
    26581 atccggcaat tggtgcacga tgcctgatgc gacgctggcg cgtcttatca ggcctacatt
    26641 ggtgccggat cggtagaccg gataaggcgt tcacgccgca tccggcaagt ggttaaaccc
    26701 gctcaaacac cgtcgcaata ccctgaccca gaccgataca catcgtcgcc agaccaaact
    26761 gaacgtcttt gcgttccatc agattcagca gcgtggtgct gatacgcgca ccggaacaac
    26821 ccagcggatg acccagcgcg atcgcaccac cattgaggtt gatcttctcg tcaatctgct
    26881 ccattagccc gagatcttta atgcacggca ggatctgcgc ggcaaaggct tcgttcatct
    26941 caaacacgcc gatatcgctg gcagaaagcc ccgctttttt cagcgccagc ttcgaggccg
    27001 gaaccgggcc gtaacccata atcgatgggt cacaaccaac gaccgccatc gaacgcacac
    27061 gagcgcgcgg cttaagacct aattcatggg cgcggctttc actcatcacc agcatggcag
    27121 ctgcgccatc ggaaagtgca gaagatgtgc ccgccgttac cgtaccgttt actggatcaa
    27181 acgccggacg cagcgtggcg agggcttcca cggtggtttc cgggcgaatc acttcgtcgt
    27241 aattaaactg cttcaggacg ccgtcggcat cgtgaccacc agttgggatg atttcatttt
    27301 taaatgcggc cgactgcgtg gcggcccagg cgcgggcgtg tgaccgcgcg gcaaaggcat
    27361 cctgcatttc acggctgata ccgtgcatac gcgccagcat ttctgccgtt aagcccatca
    27421 tgcccgccgc tttggcgaca ttgcggctca ggccggggtg aaaatcgacg ccgtgactca
    27481 tcggcacatg gcctatatgc tccacgccgc caaccagaca tgcctgcgta tcgccagtca
    27541 tgatcattcg tgctgcgtca tgcagtgcct gcatggatga accacacaag cgattgacgg
    27601 taaccgccgg gacagagtgc ggcacttctg ccagcagcgc cgcgttacgg gcgatgttaa
    27661 aaccctgctc cagcgtctgt tgcacacaac cccagtaaat atcgtcgagg gccgccgctt
    27721 ccagcgccgg gttacgcgcc agcaggctac gcattaaatg agcggagaga tcttccgcac
    27781 gcacgttacg aaaagcaccg cccttcgaac ggcccatcgg ggtgcgaatt gcatcgacaa
    27841 tgacaacctg ttccattgtg actccttaag ccgttttcag gtcgccaacc ggacgggctg
    27901 gctcaaccgg aggatagtac ggttcgttat gacgcgcttt attacgcaga ccttccggca
    27961 cttcatacag cgggccgagg tgctgatatt gctgtgccat atcgaggtat tttgcgctac
    28021 cgagggtgtc cagccagcgg aacgcgccgc cgtggaacgg agggaagccc aggccgtaga
    28081 ccagcgccat atccgcttcc gccggagtgg cgataatgcc ctcttccaga cagcgcacca
    28141 cttcgttgac catcgggatc atcatgcggg cgataatctc ttcttcgctg aaatcgcgct
    28201 tcggttggct cacttctgcc agcaggtctt caacggcggc gtcttcttct ttcttcggct
    28261 tacctttgct gtcttcttta taacgccaga aaccgaggcc gttcttctga ccaaagcggt
    28321 tggcatcaaa cagcgcgtcg atggcatcgc ggtaatcttt ctgcatccgc tgcgggaagc
    28381 ctgctgccat gacagcctga gcgtgatgcg cggtatcaat gcccacaacg tccagcagat
    28441 atgccgggcc catcggccag ccaaactgtt tttccatcac tttgtcgatc ttgcggaaat
    28501 ccgcgccgtc gcgcagcagc tggctgaaac cggcgaaata cgggaacagc acgcggttaa
    28561 caaagaagcc ggggcagtcg ttaaccacaa tcggcgtctt gcccatcttg ctcgcccagg
    28621 cgacaacttt cgcgatggtt tcgtcggagc ttttctcgcc gcgaataatt tctaccaacg
    28681 gcattcggtg gaccgggtta aagaagtgca tcccgcagaa gttttccggg cgttccagcg
    28741 cgttggccag ttcgctgata ggaatggttg aagtgttaga cgccagcacg gtatcctggc
    28801 gtactttttg ttcggtttct gccagtacgg cttttttcac tttcgggttt tcaacaaccg
    28861 cttctaccac aatatccacg cggtcaaatc cggcgtagtc gagcgttggg tggatcgtgg
    28921 agatcacgcc agccagtttc agaccatcga ttttgccgcg ctcaagctgc ttgttcagca
    28981 gtttcgcggc ttcggtcatg ccgagggtta acgacttgtc gttgatatct ttcatgacaa
    29041 ccggcacgcc tttccacgcg gactggtaag cgataccgcc acccatgatg cctgcgccca
    29101 gcacagcagc ctgtttcggg gtttcaacgt ctttggtgag tttcttcgct ttgcctttta
    29161 catattgatc gttaaggaaa atgccgacca gtgcgcgagc ttcgttggta tgcgccagcg
    29221 ggacaaaact tttgttttcc agatttaagg cttcttcacg accaaaacgg gccgcagctt
    29281 caatggtttt tactgcggtg atgggggccg gataatgttt ccccgccgtt tgcgcgacca
    29341 ttcctttagc gatggtgaag ctcatggtgg cttcaatctt gctcagtttt agcggttcct
    29401 gcttcggctg acgtttcgct ttccagtcga ggtcgccgtt aatggcctgg cgtaaaatcg
    29461 ccatcgcccc ttcaatcagt ttttctgctt tgactacgcc atccaccaga ccgattttca
    29521 gcgcctgatc cgcgccgaca tctttaccgg cggcaatgat ttccagtgca ctgtcagcgc
    29581 ccagcatacg tggcatacgt acagaaccgc caaagcccgg catgatgccc agtttggttt
    29641 ccggcagacc gatgcgcaga tccggcgtcg ccagacgata atcggtcgcc agcacgcatt
    29701 cgcagccacc acccagcgca tagccgttga cggcagcaat ggtcggcacc ggcagatctt
    29761 ccaggcgatt aaacacgcta ttggcaaagt gcagccactg acttaactgt tcttcaggaa
    29821 cgaggaacag ggacaaaaat tcggtgatat cagcaccgac gataaaggct gctttgttcg
    29881 aacgcagcag cagccctttt agatctgatt gctgttccag cacgccgatg gcctcgccga
    29941 ggctggcgac ggtcgcagtg tcgagtttat taactgaacc tggggcatca aataccagtt
    30001 cggcaatgcc atcttccagc cagtcaaggt acagggtgtc gcctttgtaa agcatgtcag
    30061 tctcctgaat ccgcaaggta atctggtcgt accagatgca tcggagtgtg tgttttgtgt
    30121 taaaaatatg caaataaaag attaaagaaa tgccgatctg atcacgctcg gcagaaatca
    30181 cgctctggat gaacgatgtg ctaagatgcg gagacttaag gtcaaaaaaa cagaagggta
    30241 aaaaatggaa tcactggcct cgctctataa aaatcatata gctaccttac aagaacggac
    30301 tcgcgatgcg ctggcgcgct tcaagctgga tgcattactt attcactccg gcgagctgtt
    30361 caacgttttt ctcgacgatc atccctatcc gtttaaagtg aacccgcaat tcaaagcgtg
    30421 ggtgccggta actcaggtgc caaactgctg gttgctggtg gatggcgtga acaagccgaa
    30481 actgtggttc tatctgccgg ttgattactg gcacaacgtc gaaccgctgc cgacctcctt
    30541 ctggactgaa gatgtggaag tgatcgcgct gccgaaagcc gatggcattg gtagcctgct
    30601 gcctgctgcg cgcggcaata tcggttatat cggtccggtg ccggaacgtg cgctgcaact
    30661 gggtattgag gccagcaaca tcaacccgaa aggggtgatc gactacctgc attattaccg
    30721 ctccttcaaa accgagtacg aactggcctg tatgcgtgaa gcgcagaaaa tggcggtcaa
    30781 tggtcatcgt gcggcagaag aagcgttccg ttctggcatg agcgagttcg atatcaatat
    30841 tgcctatctg accgcgaccg gtcatcgtga taccgacgta ccttacagca acattgtggc
    30901 gcttaacgaa cacgctgcgg tgttgcatta caccaaactg gaccaccagg caccggaaga
    30961 gatgcgcagc ttcctgctgg atgccggggc cgaatataac ggctatgcgg ctgacctgac
    31021 ccgtacctgg tcggcaaaaa gcgacaacga ctacgcacag ctggtgaaag acgtaaatga
    31081 tgaacaactg gcgctgatcg ccaccatgaa agcaggcgtc agctatgtgg attaccacat
    31141 ccagttccat cagcgcatcg ccaaactgct gcgtaaacat caaatcatca ccgatatgag
    31201 tgaagaagcg atggtcgaaa acgatctcac cggaccgttt atgccgcacg gtattggcca
    31261 tccgctgggc ctgcaggtgc atgacgtcgc cggttttatg caggatgata gcggtacaca
    31321 cctcgcggca ccggcaaaat atccgtacct gcgctacacc cgtattctcc agccgggcat
    31381 ggtgttaacc atcgaaccgg gtatctactt cattgaatcg cttctggcac cgtggcgtga
    31441 agggcagttc agcaagcact tcaactggca gaaaattgaa gcactgaaac cgttcggcgg
    31501 cattcgtatc gaagacaacg tggtgatcca cgaaaacaac gtggaaaaca tgacccggga
    31561 tctgaaactg gcgtgatgga aagctggtta attcctgcgg caccggtcac ggtcgttgaa
    31621 gagatcaaaa agagccgctt cataacgatg ttggcgcata ccgatggcgt tgaggcggcg
    31681 aaagcgtttg ttgaatcggt gcgggcagaa caccccgatg cccgccacca ttgcgtggcg
    31741 tgggtggcgg gtgcgccgga tgattctcaa cagctgggtt tctctgacga cggggagccg
    31801 gcgggaacgg caggtaaacc gatgctcgcc cagctaatgg gcagcggcgt cggggaaatt
    31861 accgctgtgg tagtgcgcta ctacggcggc atattgcttg gcaccggtgg gttagtgaaa
    31921 gcgtatggcg gcggcgtgaa tcaggcgctg cgccagctaa cgacccaacg caagacgcca
    31981 ttaaccgaat atactttgca atgtgaatat agtcagttaa ccggcattga agcgttgctg
    32041 gggcagtgtg acggcaaaat tatcaacagt gattatcagg cattcgttct gctgcgggtg
    32101 gcgcttccgg cggcgaaagt ggctgaattt tccgcaaagc tggcggattt tagccgtggt
    32161 tcattgcaat tgttagcgat tgaagaataa tccccacttc gttttgcaga actaaggaag
    32221 cggcagagat gcattttcgc gccattaccc gaatcgttgg actactggtc atcttatttt
    32281 cagggaccat gattatccct gggctggtag cactcatcta ccgggatgga gcgggccgcg
    32341 cttttaccca gacctttttt gtcgccctcg ccattggctc tatgctgtgg tggccgaacc
    32401 gcaaagagaa gggcgaactg aaatcccgtg aggggtttct gatagtggtg ctgttttgga
    32461 ccgtgctggg tagtgtcggt gcgctccctt ttatcttctc ggaaagcccg aacctcacga
    32521 ttaccgatgc gttttttgaa tctttctctg gcctgaccac caccggggcc actacgctgg
    32581 tggggctgga ttcgctccct catgccatcc tcttttatcg ccagatgctg caatggtttg
    32641 gcgggatggg gatcatcgtg ttagcggttg cgatactgcc tatcctcggc gtgggtggga
    32701 tgcagctcta tcgcgcagaa atgcccggcc cgctgaaaga taacaaaatg cgcccgcgaa
    32761 ttgcggaaac ggcgaaaact ctgtggttga tttatgtctt gctgaccgtc gcctgtgcgc
    32821 tggcgttgtg gtttgccgga atggatgcct ttgatgccat cggccatagc tttgcgacta
    32881 tcgctattgg cggcttctcg acacatgatg ccagtatcgg ttatttcgac agtccgacta
    32941 ttaacactat cattgctatc ttcctgctga tctccggctg taactacggt ctgcactttt
    33001 cactgttaag tgggcgtagt ctgaaggttt attggcgcga tccggaattt cgcatgttta
    33061 tcggcgtaca gtttacgctg gtggttattt gtacactcgt actgtggttt cataatgtct
    33121 acagttcggc gctgatgaca attaaccagg cgtttttcca ggtggtatcg atggcgacaa
    33181 ccgccgggtt tacgactgac agcattgccc gctggccgct ctttttgccg gtactgcttt
    33241 tatgttcagc ttttatcggc ggttgcgccg ggtcaacggg cggtggcctg aaagtgatcc
    33301 gcatcctgct gctgtttaag caggggaacc gtgagctgaa acgactggtg catccgaacg
    33361 ccgtctatag cattaagctg gggaatcgcg cactgccgga acgtatcctc gaagccgttt
    33421 ggggattttt ctccgcctat gcattggtgt ttattgtcag tatgctggcg attatcgcca
    33481 cgggcgtgga tgatttttct gcctttgcct cagttgttgc gacattgaat aacctggggc
    33541 cagggcttgg cgtggttgct gataacttta ccagtatgaa cccggtggct aaatggatcc
    33601 tgattgccaa catgctgttt ggtcgtctcg aggtctttac attactggtg ctctttaccc
    33661 cgactttctg gcgtgaatga tggagtaata cgtgaaaaca ttaattcttt tctcaacaag
    33721 ggacggacaa acgcgcgaga ttgcctccta cctggcttcg gaactgaaag aactggggat
    33781 ccagacggat gtcgccaatg tgcaccgcat tgaagaacca caatgggaaa actatgaccg
    33841 tgtggtcatt ggtgcttcta ttcgctatgg tcactaccac tctgcgttcc aggaatttgt
    33901 caaaaaacat gcgacgcgac tgaattcgat gccgagcgcc ttttattccg taaacctggt
    33961 ggcgcgcaaa ccggagaagc gtactccaca gaccaacagc tacgcgcgga agtttctgat
    34021 gaactcgcaa tggcgtcccg atcgctgcgc ggtcattgcc ggggcgctgc gttacccacg
    34081 ttatcgctgg tacgaccgtt ttatgctcaa gctgattatg aagatgtcag gcggtgaaac
    34141 ggatacgcgc aaagaagttg tctataccga ttgggagcag gtggcgaatt tcgcccgaga
    34201 aatcgcccat ttaaccgaca aaccgacgct gaaataagca taaagaataa aaaatgcgcg
    34261 gtcagaaaat tattttaaat ttcctcttgt caggccggaa taactcccta taatgcgcca
    34321 ccactgacac ggaacaacgg caaacacgca gccgggtcag cggggttctc ctgagaattc
    34381 cgacagagaa agcaaaaata aatgcttgac tttgtagcgg gaaagcgtat tatgaacacc
    34441 ccgcgc
//
LOCUS       DAJTSI010000029        33711 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000029 DAJTSI010000000
VERSION     DAJTSI010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33711
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.029"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..2047)
                     /gene="recB"
                     /locus_tag="OO327_001248"
     CDS             complement(<1..2047)
                     /gene="recB"
                     /locus_tag="OO327_001248"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR6017862.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     "
     gene            complement(2040..4928)
                     /gene="ptrA"
                     /locus_tag="OO327_001249"
     CDS             complement(2040..4928)
                     /gene="ptrA"
                     /locus_tag="OO327_001249"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR6017863.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(5104..8472)
                     /gene="recC"
                     /locus_tag="OO327_001250"
     CDS             complement(5104..8472)
                     /gene="recC"
                     /locus_tag="OO327_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR6017864.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(8485..8835)
                     /locus_tag="OO327_001251"
     CDS             complement(8485..8835)
                     /locus_tag="OO327_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR6017865.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVLMVLMVMIVTALSGIQR
                     TSMNSLASRNQYQKLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGG
                     REGEMTRLHCPNCQ"
     gene            complement(8820..9227)
                     /locus_tag="OO327_001252"
     CDS             complement(8820..9227)
                     /locus_tag="OO327_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR6017866.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(9224..9787)
                     /locus_tag="OO327_001253"
     CDS             complement(9224..9787)
                     /locus_tag="OO327_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR6017867.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(9778..10248)
                     /locus_tag="OO327_001254"
                     /pseudo
     CDS             complement(9778..10248)
                     /locus_tag="OO327_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(10432..11226)
                     /gene="thyA"
                     /locus_tag="OO327_001255"
     CDS             complement(10432..11226)
                     /gene="thyA"
                     /locus_tag="OO327_001255"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR6017868.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(11233..12108)
                     /gene="lgt"
                     /locus_tag="OO327_001256"
     CDS             complement(11233..12108)
                     /gene="lgt"
                     /locus_tag="OO327_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR6017869.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(12259..14505)
                     /gene="ptsP"
                     /locus_tag="OO327_001257"
     CDS             complement(12259..14505)
                     /gene="ptsP"
                     /locus_tag="OO327_001257"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6017870.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(14518..15048)
                     /gene="rppH"
                     /locus_tag="OO327_001258"
     CDS             complement(14518..15048)
                     /gene="rppH"
                     /locus_tag="OO327_001258"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR6017871.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            15733..16422
                     /gene="mutH"
                     /locus_tag="OO327_001259"
     CDS             15733..16422
                     /gene="mutH"
                     /locus_tag="OO327_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR6017872.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALDGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            16491..17204
                     /locus_tag="OO327_001260"
     CDS             16491..17204
                     /locus_tag="OO327_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6017873.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            17342..17560
                     /gene="ygdR"
                     /locus_tag="OO327_001261"
     CDS             17342..17560
                     /gene="ygdR"
                     /locus_tag="OO327_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR6017874.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            17668..18708
                     /locus_tag="OO327_001262"
     CDS             17668..18708
                     /locus_tag="OO327_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR6017875.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(18741..19931)
                     /gene="lplT"
                     /locus_tag="OO327_001263"
     CDS             complement(18741..19931)
                     /gene="lplT"
                     /locus_tag="OO327_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR6017876.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(19924..22083)
                     /gene="aas"
                     /locus_tag="OO327_001264"
     CDS             complement(19924..22083)
                     /gene="aas"
                     /locus_tag="OO327_001264"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR6017877.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            22669..23700
                     /gene="galR"
                     /locus_tag="OO327_001265"
     CDS             22669..23700
                     /gene="galR"
                     /locus_tag="OO327_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR6017878.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(23707..24969)
                     /gene="lysA"
                     /locus_tag="OO327_001266"
     CDS             complement(23707..24969)
                     /gene="lysA"
                     /locus_tag="OO327_001266"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR6017879.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            25091..26026
                     /gene="lysR"
                     /locus_tag="OO327_001267"
     CDS             25091..26026
                     /gene="lysR"
                     /locus_tag="OO327_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR6017880.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(26013..26705)
                     /gene="ygeA"
                     /locus_tag="OO327_001268"
     CDS             complement(26013..26705)
                     /gene="ygeA"
                     /locus_tag="OO327_001268"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR6017881.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(26834..28252)
                     /gene="araE"
                     /locus_tag="OO327_001269"
     CDS             complement(26834..28252)
                     /gene="araE"
                     /locus_tag="OO327_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCR6017882.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(28567..29328)
                     /gene="kduD"
                     /locus_tag="OO327_001270"
     CDS             complement(28567..29328)
                     /gene="kduD"
                     /locus_tag="OO327_001270"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR6017883.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(29358..30194)
                     /gene="kduI"
                     /locus_tag="OO327_001271"
     CDS             complement(29358..30194)
                     /gene="kduI"
                     /locus_tag="OO327_001271"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR6017884.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(30481..31662)
                     /locus_tag="OO327_001272"
     CDS             complement(30481..31662)
                     /locus_tag="OO327_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR6017885.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            31917..33146
                     /locus_tag="OO327_001273"
                     /pseudo
     CDS             31917..33146
                     /locus_tag="OO327_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT         8448 a         9076 c         8105 g         8082 t
ORIGIN      
        1 gccgctcacc gcctgccgtt gccagcaagt tttctgcaat gttacgcgcc gacatcagcg
       61 cccgcagcat cggcataacg ccacgtttgc gccagatttg ccgataacca tcgaactctt
      121 cgactaccgc atcccacgca tgttcgtcat tattcagcgt ttcaatatcc agcgcattca
      181 gccccatcat tgacgttgcc agcgcactgc gcagggtgtt ctcacgttcg ggcgtcatca
      241 ccgcctgcaa caaccaaagc atttcctgcg cttccagagt ttcaaaaaca ctgtcgcggt
      301 tcgaaaggta aacggaaggg atttccagca acgttaaggc atcgcgcacc tgggcggcct
      361 cctggcggct gcgcaccagc acgctgatgt ccgaagcacg caccggacgc gcgtcgtcgc
      421 cgttcatcag caacgcttcg ccccgctgtc cggcttgtag ccagtcgcgg atttgcgcag
      481 cacatacctg cgccatggta ctttgatagt cgccaacgcc gcagctttcg ccttccatca
      541 gccacatttt catcgcaggc tgcgtttcac ctttaaatac aaaacgtaac gcctgatttt
      601 tcccggctga tttcactgga ataaacggta tttcgcgaaa catgaacgtg tcatcagtct
      661 ggctgaaaag cttgttcaca ctgttcacca ttccaggtgc ggagcgccag ttggtgtcta
      721 aagtgtagtg ggcatgaact tcactacgcg ccttcatata agtgaagata tccgcacccc
      781 ggaatgcata tatggcctgc ttcgggtcgc caatcagcaa caatgcggtt tccggctgat
      841 ggtgccagat acggcgaaaa attcggtact gctgggggtc ggtatcctga aattcatcga
      901 tcattgccac cgggaatcgc gtacggatcg ccgctgccaa cacctcgccg ctttcgctac
      961 gcagcgcgga atcgagccga cttaacatgt catcaaaacc caattcgcca cggcggcgtt
     1021 tttcacgcgc tactgtttcg cggatctcag ccaatgcgcg ggtgatcaac agatcgcgga
     1081 tcgacaatgg ttctgcaagc agttgatcga tcgcctcaaa cagtggatgt cgcggggttt
     1141 cccccccggc cttcgtgcga tcttctaaga aacgctggga gaatttttcc agcgactccg
     1201 gcaactgata actgtttgtc tcttcttctg cccaggcgct gatcttttcg atccatttag
     1261 cctgattgct acggttaaac ttgcgtcgat caataccaga agattcgatc agcgcatcca
     1321 gttcacccac tgcgtcgcgc cactgctgtt ttaccgtatc aatacgcgcc acaatttgcg
     1381 cgtggcggga agccagcgtt tcatcatcgg gcggcggtgc tttgataacc ggcggttcgc
     1441 cttgcagata acgattaata tcgcgcagca acgcctgcgg ccctttccag gtttcaaaga
     1501 cgacctgggc tatttcacgc ggcagcgggt agcagtggcg acgccagaaa tcggcgcagg
     1561 cctggtagcg tagcagagac tcatcttcaa tcagctgctg ctcaaacagc atgccggatt
     1621 caaaggcatt caggttgagc atgcgctggc aaaagccgtg aatagtaaag actgccgctt
     1681 catccatctg ccgttcggct aacaacaacc actgcgcggc ttgcgcttta tcgtcgatct
     1741 cttccagcag gcgttcgtac agtggattgt cggtggtttc acgcagacag gcgatgcgca
     1801 actcgtggat attgctacgg atacgaccgc gcaattctgc cgtggcagcc tcggtaaagg
     1861 tgaccaccag cagttcttca acgatcagcg ggcggggaaa ggcggcggaa ccgcctagtc
     1921 caagtaacag gcgcaaatag agcgccgcaa tcgtaaaggt tttgcctgtg ccggcagagg
     1981 cttcaatcag gcgctcgccc tgtaagggca agcgcaaagg atctagtgtc tcggcgacat
     2041 cactcattct tttcactcat caggggcatt gtttgctgca acgcgctgac gttctcccac
     2101 actttccagc cttcaggatg cacatattcg gctttcccgt tctggctgcc ggaaatctgc
     2161 gacagaatag ccatgccttg cggctcgacc accgcctgat ggaagaaatc agcaagtttt
     2221 tgcggcgtca gcagttttat ctgggccacg attttatcac gcgaatcgaa gcgcatattg
     2281 ccgcgatcga aatctttact taacttcgat gcttcttcgc cgagcgtttg cggtgcctgc
     2341 agcatctggg taattaccgc ctgctggatt tgcgcaaact catctggctt catcgctcgc
     2401 aattttgcct ctgcggttgg gaaaaacgcc ttgtaacgct cccacaagaa tgaaggctgt
     2461 ttatcattgc tttgcaaaag gaagcccatg ccccactgac gccccacgct cattggaaac
     2521 gcaaacacgg catagcccaa ttgttcttcg gtacgcaact gattgtagaa ccacggctgt
     2581 acgatctgcc ccaacagaga gctataggct gagctggtgt attcatcgta gccagtcggt
     2641 acaaataccg ctgccagtgc ggagtcggtg ctgttaccgg ctttttcaaa gatgacggat
     2701 tgttttttat cgaccactac atctttgttt cgacaccact ctgaaccatc agcgcccaac
     2761 tgtttttgca catcgcgtgc cagcgttgtt gcctgggcct cggtcatgtt gccgataacc
     2821 ataaactctg gtcgagcccc tgattttaag gcgtcgcgat aggccagcac ctctttcaac
     2881 gtaatggagg gcaaaatttt acgccgttca tctcgcgaga agtacggcac ttgcgagagc
     2941 atctgcgcgg gcataatcgc ctgctcaaac gctttaccct tttctgcgga atccatcatc
     3001 tggttatacc aggacttcgc ctgctcaagc tgctcttccg tagcggtata gctaaagtac
     3061 ccctcgagca atgcctggaa cagctgcggc agacgctggg tgtaaccatt agcattaatc
     3121 ataaggccgt tgttagcgtt ggtggaaaaa cttatgccac caaccgacgc ctggttgctt
     3181 aactgatcaa gcgccagccc tgcgagataa tcattgagcg caaacatcac ctgattgcgg
     3241 gcgctgtcca tggctttcgg attacgcaaa atcaggctga catcagcttt gggctcgctg
     3301 gcaaaataac ggcttggcgc atacaccacg cgcagattcg actcatcaac aatcagctct
     3361 ggatggtcgt atttcttctc tgacttaatc agcgagaaat catcaggaat ataagggtta
     3421 agctctggca aagagagcgc aatgtcggcg gcttttttct gccagtcggc gaaagtttgt
     3481 gcgctgattt tatcgacctg atacggcgca tcgacaaagt aagccgtttt gttgtgcggc
     3541 tctttcgggc tgatatacca gatacgcgca ttctgcggcg tcatcatcgc cagacgttcc
     3601 tttactgctt tagcatcgta ccgatcggca atattgactg catccagcgt atgctcaaca
     3661 ggaacgcgaa tcatggtatc tgccagccat tcgacgtaat ccatatcacg ggtgatcgac
     3721 ggataacgga agtcgatatc cagcacattc gccagttcat cgaagtattg tttatcaatg
     3781 cctttttcac gtaacagatt gagatagcta aaaattgccg ccacaacctg atcgcgatta
     3841 gccaggcctt tatcagttaa agacgcagag atcgctaata cgccgctgtt gccgttgacg
     3901 ataggatcgg agttggcgct aatgccctca actaatccct gcttttgcag ccagtcagaa
     3961 agtgtacctg ggctgcgatt gccaatcaga taggtaatca attcatcggt tttactacgg
     4021 aacttcgctg agttgttatc gatgcgaaac tcaacgcgca acactttacg cggcagcgca
     4081 gggacgtaat gaatgataat gcccttttgc gcgtcggtga ctaccggcac ggtgatttcc
     4141 ggttttttgc tctctttgtt cggcacgcga ccaaaggtgt ccgccgccat ttttgccaac
     4201 tccggcagcg gtttattact gtaaataacc gccttcatca aattggcgga atagtacttc
     4261 tcgtggaaat ctttcagcgc ctgctgcacc ggattaccag gtttgtcgct taaagtttcg
     4321 aggttaccac cagaaaactt tgaaccgggg tgtgccgggt taatggtttc tgcgctgacc
     4381 tgtgccatgc gcatcccgtc acgcgtacgc gccatggtta attcagcgtt cactgcatta
     4441 cgctcacgtt cggcgtattt cttgtcgagc aagggttctg caatagcatc ggccaggcgg
     4501 tctaccgcac cgggcaaggc gtcgttctca acttccagat agaaagccgt gcgatacggc
     4561 gcagtgctgg cattgtgact accgccgtgc attttgagat attcggccag actgtcagcc
     4621 tgcgggtact ttttcgaccc catcagactc atatgttcaa ggtaatgtgc cagcccctgg
     4681 tacgcctcgg gatcttccag cgacccaacg ggcactacca gcgccgagag cgatttaact
     4741 gcctgcggat cagaaaccag caagaccacc ataccgttat ccagacgtat agcctgatac
     4801 tggcggttat ctttatcact tttacggatg gtttcctgaa tcggctgcca tcccgtttct
     4861 gcctgactta atggtgccca gagggcaaca aacaacaata atgctttgaa ccaggtgctg
     4921 cggggcattc acggacctca taatcaactt tattttctgg acagattcaa caggttaacg
     4981 caaatcatct gccagaattt aatcttgtgc tgcacgagtc agcctatgtt tatataacca
     5041 tcagtccgtg actggtgcgc atcataaagt aagcagatag attgcgcaat ttttatacaa
     5101 cactcatgac tgattaaagc gaaacagcgg taacaggaaa cgttgcgact gttcaacgat
     5161 ggcctccatt gtctctggtg ttaattgccg ccagagccgt tgataccaga tatcatcacc
     5221 ttcgccacgc accatcatgt tgccttcgta agcctgaagg aatttcgtac gggctttttg
     5281 caacgtggaa tcgtcatcca gcatggcatc gttttgcgcg tcataacagg tttttagcca
     5341 cgcgccgcca ctttcaggta acaccagcaa tggcgcggac attccttcac gatacccctc
     5401 aatcagttgt gagaggtaat gcaaagcctg ttcggctgca agcggcggaa aacgccactc
     5461 accgtctttg cgtagaaaaa ggcgactttc gccattacca ccgctggcac agtagacaag
     5521 gtgttccagc caaagttgca ttccctgcgc cacacttaat aaagagggac gccagcgcaa
     5581 caggccatcc ggctgcacct gcggcaacca gccagttatc tgcacaccgt tgcaggcgag
     5641 atcaatttcc atactctgcc ccggctggcg acaggcaatg actctgtcgg caagctgctg
     5701 catctcctgg cactgtgttt cccagaaaat ttcaccaaaa gcgccatacg gtaaatcccc
     5761 tgccgcccgg aagcggcgga acaagcgttc ggcatcatcc tgctcaacca gtgcattcaa
     5821 taactgctga ttgatttgat aacggctaag tccttccaga ataaatggct cggtgtctgg
     5881 gatttcgctg tcttcagtat ggaagttcac ctgcaaacgc atctggaaga acgcccgcac
     5941 cggatgtgcc cagaatcgtt gtagcgtttc cagcggcacg gtttccggta aggtaaacgg
     6001 cagcggctga acaaattcag aatgtgcttt accagcctgg ctggccgcag gtagccattc
     6061 acgagcatag ctttgtcgtt cgcctggctg gtagttttgc ggatcaaacg gcatccgggt
     6121 atggaggcaa gtaagatgcg cttttaccct tgcctcgctt tcatcacagt tgagcgcttc
     6181 atcgcccggt agataatgac tttgcccgat gtagtcgatc agttcctgca ccagtaccga
     6241 cgggaaacgt tcactgttat cctgaatgga acgcccgata tagctgatat agagtttttg
     6301 ctgcgcggaa attaacgctt ccaggaacag gtagcggtcg tcatcgcgac ggctacggtc
     6361 gccacgcttc ggtttctggc tcatcaggtc aaagcccaat ggcgcaagct gacgtggata
     6421 aacgccgtcg ttcattccca gcaggcaaac cactttgaac ggaattgaac gcattggcat
     6481 cagagtacaa atgttaaccg gtccggcgag aaaacgctgg ctgatacgtt cttgatccag
     6541 acgctgtgcc agttcatcac gcaatagtga cagcggcacc gcgtcgccat actgcgcacc
     6601 taaaccttcg gcgataatcg cctgccattg ttgttcgatc agcgtcatcg ccgcttcggt
     6661 ttccgcatcc ggcaggaaga aggcgttgag catatcgcga caaaccggca accactcttc
     6721 tagcggacgc tcctgtgcca gcccgcgacg ccagatgttt agctgcatta gcagtgaagc
     6781 cagatgcccc accagttctg caattaagcc gctcgattca tcataaggta gaaccgattg
     6841 ccactcgccc tgcgcgctct ccatcgcgta gcccagcaac atacgcgtca ggccaaatcg
     6901 ccaggtgtgt tgtccggtgg cggggagttc cagctcgcga acgttgtcgt catctatccc
     6961 ccaacgaatg ccggattcgt tgacccactg gcgtaaataa cgcagccctt cttcggtgat
     7021 gtcaaaccgc gccgccagca ccggcacatc cagcagcgcc agcacatcct ctgacacaaa
     7081 gcgactgtca ggcagtgata acaggctgat aaacgcttcc agtaccggat gtgactgccg
     7141 cgcacgacgg tcggaaatgg cgtaaggcag gtaacgatcc gcaggtgcgc taccaaacac
     7201 tgcctgaata aacggactgt agctgtcgat atcagccacc atcacgatga tgtcgcgcgg
     7261 agtaagtgtc ggggcttcct ccagcatcgc cagcaagcga tcgtgtaaaa cttcaacttc
     7321 acgctgcggg ctatggcaaa cgtggaatgt gatactgcta tccagtggat caagcgggcg
     7381 tttgttatcg ctacgggaaa actcttcgat gttcacaccc gcaacggcgc ggttttccag
     7441 ttccagaatg tcagactgaa tgttatgcag caggttatct ggcgtcacat cgacaaaagc
     7501 gtccagctcc tggctgctct ccaggtcaga aaggagataa atgtagtcgc gcccaagctt
     7561 cccccatgag gccagcagcg agttgccgac atcctgttca ccatcgctgt taaagagctg
     7621 tccggcattt tcgctatcgc gaaatagcgg caattcgcga tcttcaaaac tgtgtcgacg
     7681 ctgacgagtc agcagtttcg ccagataagc aggatcttta atatcgcccc agtaataacg
     7741 gcaggggttg gtaaacagga gatggatttc aatatgttta cccagcgcct ggagcgcctg
     7801 gagataaaca ggcggtaacg cggaaatacc gcatataaag acgcgcgaag gtaaccccgg
     7861 cgggcaggtc gtcgcggact ccagcgtttc gataaagcgc tgatagagat tcgcgcggtg
     7921 ccagcgcggt tgtccgagtt catgggtata ttccaccagc gccttccaca acggggcttg
     7981 ccaggcctgt gcttctccca gcccttcaac caaatgtcct gtttcccact gtgccagcca
     8041 gtccggacga tagaccagat actggtcaaa caggtccgcc gcttttgagg aaagctggaa
     8101 cagttttcgc ttgtcgctat cgtcagtcag ataatgccgc aacagggtaa agtcttcgcg
     8161 ctccagcaat tgcggcagca gagtcatcag tttccagctc atgctctgtt tgttaaaggc
     8221 gctctctttg gggatttccg gtaacacccg gacgaacata tcccagataa agctcgctgg
     8281 cagcggaaaa tcaatgtttg ccgcaatacc aaacttttgc gacagggtca tttgcagcca
     8341 ctgcgccata ccggtacttt gcaccagaat catctctggt tcgaaaggat cgtccagccg
     8401 ttcgcgttcg acaataaact ccatcaacgc ttccagcacg tccagacgat tggaatggta
     8461 gacccttaac atagcggctc ctgactactg acaattcggg caatgcaggc gggtcatctc
     8521 gccttctctg cccccgggtg aaactagcgt aacgctgatg ctgacacatc ccgcctgcga
     8581 tgtctgcatt cggttgacct gccagttggc aggtggcgaa atcgcgcgca gttgcgtttg
     8641 ctgccaacca tgccgccaga gcttttggta ctggtttctg ctggcgagac tgttcattga
     8701 tgttcgctgg atacccgata acgcagtgac aatcatcacc atcagcacca tcagcaccat
     8761 cagcaccatc agcaccatcg caaccattac ctccggcagg ctaaagcctt gttgattctt
     8821 cagggaagct gacataatgc cccctctttc agcggacaaa aatcgctcca gccgcgtggc
     8881 gaaaaaacaa tttttccatc aatgacttcg cctgttcgcc acaacgaaac gccttcataa
     8941 ccggcaatca ataaggcttc attatctgcc agtaaacgca aacaaacctg ggcatcggtt
     9001 ccagcgtact gcgagcactg aactgctggc tgcgtctgcc aggagtgcat ttttccccac
     9061 gccagcgccg actgaacgat ggcctggcgg cgcaatgact gactttccat gctcacgcga
     9121 gaggcaaaac tgcgatcctg ctgactcatt ccttgcaata gcaagctacc caaaaccagc
     9181 agcatcagga ccagagccag tgacgaaaca cctttttcgc ggttcacagg ttgaatcctg
     9241 tcacgctata gctggcatcc accacggttt gcggttcaga cttactggca gcacgcatat
     9301 taaccgtcaa caccggcgag aagccgctga catcctgacg tacgacctga aaagtgtcga
     9361 taatgatggc atccggatta gtgactttat cccagccctt accttcacag gatgtcgcac
     9421 cgcgtagcgt ttccagcaca tgctccttca gacgaaatcc aatctggtcg gactctttta
     9481 ccggttcgcg atcccagata ccgttactgt tcgcatccca ctgcacaatg atacagtcac
     9541 cctgtccgac aatttccagc ccttcgccgg tacagctgcc atgacaataa cccgccctct
     9601 ggagatgctt cgcgacggta aatacccgca gccagatttc atcttccagt gccagcttac
     9661 gagtgctcgt taaactttca cgctgtaacg caggcagaaa gcgtgcagcc cccagcaaca
     9721 atacgctact gatcgccata gcaatcaaca cttccagcag agaaaaacct tgctctttta
     9781 caggcatcct tctgtttctc cttgctgata aagccggagt cttccccacg gcgaaaccac
     9841 cagccaccac tcgcccgttg agtttttgaa gcgaatatgc acggcccatg cggtattgcg
     9901 caggccaaag aaagcaagcg aaggtgtcag gtcgctcatt tcgacttcgg gccagcgtgg
     9961 cacaaagacc aatggtgaac tgccatgaca ggtattggcc ccagcagcgg aactcacaag
    10021 gcaccataac gtcccctccc tgataacgct gatactgtgg tcgcggttat gccagttggc
    10081 atcttcacgt aaatagagca aatagtcccg cgcctagctg gcggtttgcc aaagccgttg
    10141 cgactgctgc cagtattgcc agccatagag tccacttgcg cttagcatga ccaaaatcag
    10201 catcgcgacc agcgtttcaa tcagcgtata accacgttgt gttttcatgc cggcagtatg
    10261 gagcgaggag aaaaaaagac gagggccagt ttctatttct tcggcgcatc ttccggacta
    10321 tttacgccgt tgcaggacgt tgcaaaattt cgggaaggcg tctcgaagaa tttaacggag
    10381 ggtaaaaaaa ccgacgcaca ctggcgtcgg ctctggcagg atgtttcgta attagatagc
    10441 caccggcgct ttaacgcccg gatgtggatc gtagccttca atctcaaagt cttcgaaacg
    10501 gtagtcgaag atggattcgg gtttacgttt gataatcaac ttcggcagcg gacgcggttc
    10561 gcggcttaat tgcagatgag tttgatccat atggttgctg tacaggtgcg tatcgccacc
    10621 ggtccagaca aaatcaccca cttccagatc gcactgctgc gccatcatat gcaccagtaa
    10681 cgcgtagctg gcaatgttga acggcaggcc gaggaagacg tcacaggagc gctgataaag
    10741 ctggcaagag agtttgccgt ctgccacata gaactggaag aatgcatggc acggtgccag
    10801 cgccatttta tccagttcgc ctacgttcca cgctgaaaca ataatgcggc gcgaatccgg
    10861 gtcgtttttc agctggttca gtaccgtagt aatctggtca atatgacgac catctggcgt
    10921 tggccaggcg cgccactgtt taccatacac tggcccgagg tcgccatttt catcggccca
    10981 ttcgtcccag atggtgacat tgttttcgtg tagataagca atgttagtat cgccctgcag
    11041 gaaccacagc agttcgtgga tgatggaacg caggtggcaa cgtttggttg tcaccagcgg
    11101 gaatccatcc tgcaggttaa aacgcatctg atgaccaaaa atggaaagcg ttccggttcc
    11161 ggtacggtcg tttttctgtg tgccttcgtc gagcactttt tgcatcagtt ctaaatactg
    11221 tttcatggtt cctcaggaaa cgtgttgctg tgggctgcga cgatatgccc agaccatcat
    11281 gatcacaccc gcgacaatca tcgggatgga aagaatttgc cccatgctga tgtactgcac
    11341 ccaggcaccg gtaaactgcg cgtcgggctg gcggaaaaac tcaacaatga tgcgaaacgc
    11401 gccgtagcca atcaggaaca agcctgagac agctcccatt gggcgcggtt tacgaatata
    11461 caggttgagg ataataaaca gcaccacacc ttccagcagc agttcgtaaa gctgtgatgg
    11521 gtggcgcggc agcacaccgt aagtgtcgaa aatggattgc cactgcgggt tggtttgcag
    11581 cagcaaaata tcttctgtac gggagcctgg gaacagcatg gcaaacggga agttcgggtc
    11641 aacgcggccc cacaattcac cgttaataaa gttgcccaga cgcccggcac caagaccaaa
    11701 cggaatgagt ggtgcgataa aatcagagac ctggaagaag gaacgtttag tacggcgggc
    11761 gaagataatc atcaccacga taacgccaat caggccgccg tggaaagaca tgccgccgtc
    11821 ccagacacgg aacagataca gcggatcggc cataaactgc gggaaattgt agaacagaac
    11881 ataaccaata cgtcccccga ggaagacgcc gaggaagccc gcatagagta agttttcaac
    11941 ttcatttttg gtccagccac tgcccggacg attcgcccga cgtgttgcca gccacattgc
    12001 aaaaatgaac cccaccagat acatcaggcc gtaccagtga agcgccacgg gtcctattga
    12061 gaaaatgacc ggatcaaact ccggaaaatg cagatagcta ctggtcatct gtcaccacaa
    12121 gttcttgtta tttcgctgaa agagaacagc gattgaaatg cgcgccgcag gtttcaggcg
    12181 ctccaaaggt gcgaataata gcacaagggg acctggctgg ttgccggata ccgttaaaag
    12241 atatgtatat gatccgcgct ataaccctcc gcgaatcagc ccgcccatgc cgcgacgctc
    12301 cataaaggct gcaacctgat gacgaacttc ggtcgccagt tgcgcttcca gactacgctg
    12361 cgcaagattt tctgcttcgg caaaatcaat gcgccgcagc aggtatttta cccgcgctac
    12421 agaacgtccg ttcatagaca gatggcgata cccaagccca atgaggattg ccacgcacat
    12481 gggatcgccc gccatttcac cgcacaaacg aagatcgata ccatgtattt ccgcttcccg
    12541 ggcgatcatc gccagagctc gtaacattgc aggatgaaga ctgtcgtaaa tgttcgccac
    12601 ccgggtattg ttgcgatcaa cggccagaat gtattgagtc agatcgttgg tgccaacaga
    12661 gatgaaatcg accctctttg ccagatgcgg cagcataaat accattgacg gcacttccag
    12721 catgatgccg atacgtggtt tgggaatttc gtaaccgatc atctcctcga cttcacgtcc
    12781 ggcacgttca atcaggcggc gtgcttcgtc aacttcatcg aggctggtga ccatcggcaa
    12841 cagaatattc aggttgcccg tagcggcatt agcacgcagc atcgcccgca cctggatcaa
    12901 gaagatctcc ggctgatcga gcgtaatgcg aatcccacgc caacccaggc atggattctc
    12961 ttcgctgatc ggcatgtaag gcagctgctt atctgctccg acatccagcg tacgcaaggt
    13021 gacgggttta tcattgaaca tttgcagcat cccctgatac tgcgccacct gttcttcttc
    13081 cgacgggaaa ccactttgca gcatgaatgg gatttcagtg cgataaagac cgatgccatc
    13141 aatacagctg cccagttttt cttcatgttc cgggcttaaa ccagcattga gcatgacttt
    13201 tatacgctca ccgcttttta actgcgcggg taaattgacg tcatcttccg ccagacggct
    13261 aagctcaatc tcttcactaa ttagccgctg atattcttgc agcagtaccg gctccggatc
    13321 gaccagcaat tcaccgcgat agccatcaac gatcagcgtc ctacgatgca gcaccgaagg
    13381 ctgaatatcc gcgcccatca cggtagggat ccccagcgca cgtaccatga tcgcagcatg
    13441 ggagttggct gcgccatctc gcacgacaac accgactaag cgatcctggg gcagctcagc
    13501 aagagttgtc gctgacagtt catctgccac cagaatgaaa cgttccggcc aggcgttcgg
    13561 cccttgatta gcgtcatcaa gatgaaacag caatcgctga cccagcgcac gtaaatcacc
    13621 agcccgctct ttgagatagt tatcgcttag cgcggcaaac tgttcggcaa atttttcaat
    13681 gaccgttttt accgcccact ctgccaccga gcctttatca acctcggcaa acaattcgcg
    13741 acgcagccgg gtgtccgaaa gcaggtgcga gtaaagatcg aaaatagccg ccgtttcttt
    13801 ttgtgctccg gcggcaaagc gtttgctgta gcggcgaaac tcgtttgccg cttcttccag
    13861 cgccccggtc agtcgttcgc gttccagagc cggatccagc gttgatgcct gatacacctg
    13921 ttccattaaa ggtaacgtgg catcctgcca gccttcggca atcgccacac caggtgctgc
    13981 cggtaatgcg cggattcgcg tctggcgata ttgcccaaac agggcagtca actgcgactg
    14041 agaaagaata gctgccatct gggtggcaag cgtcaccagg aaggattctt cactttcgtc
    14101 atactggcgc aactctcgtt gctgtaccac cagtacacca agcaactggc gacgttgaat
    14161 aattggtacg cctaaaaacg cgcggaaacg ttcttctttt acggagggga tgtatttgaa
    14221 gctggggtgc ttttgcgcat ctgcaaggtt tatcggttcc gccagcctgc caacaaggcc
    14281 gacgatccct tcatcaaacg cgagcgttac agtgcgaccg cgtggttttt tcagcccccg
    14341 ggtcgccatc aggtagtaac aacgtcgatc atgatcggcc aggtagaccg aacagacctc
    14401 ggtatccatc gcaagacaga tgtcggtaac cagaatattt aacgcctcat tcaggcgtgg
    14461 tgcgctggct accttttcga ctatttcgcg caggcgagtg agcataattg gcgtgactta
    14521 acctctttta cgtcgataag cagatgcgtt ttgtggtttt ggcgtatttt cctgcagtga
    14581 catcaccaca ctcgcgaact ctttcattac cctacggtag acatcacgtt taaatgacac
    14641 cacctgtctg accggatacc agtaacttac ccatcgccag ccgtcaaact ctggtgtact
    14701 gctggtttgc atattgattt ctgcatcgcc gcttaccagc tgcaagagaa accatttttg
    14761 tttttggccg atacaaaccg gcttcgtgtc ccaacgcacc aaacgtttcg gtaatttgta
    14821 gcgcaaccag ttacgcgttg aagcaaggat tcgaacgtct ttgcggctta atcctacttc
    14881 ttcaaacaat tcacggtaca tcgcctgctc tgcggattct ccggggttga ttccgccttg
    14941 cggaaattgc caggagtgct gaccaaatcg ccgggcccac attacctgcc cctggcgatt
    15001 acaaatcacg atacctacgt ttgggcggta gccatcgtca tcaatcaccg gactacctca
    15061 aaataaagct ttatatacga atgattgttt catactccag gaagacggta aaccactctc
    15121 tgcagggcat tacacactaa taacaattga ataactcaca gttatgtgca gagttataaa
    15181 cagaggaagg ggtggatagc cgtttttatt caccttttct gtggatagag ttgtgaagaa
    15241 ctacggaatt actacgggaa aacccggagc attctgaata agcccgatat agacatttta
    15301 aaatattcat tattagtcaa tgcattacat gtaatttgag taatgaaaaa gagtaattcg
    15361 tgacccaggt cacacctctc atttacgggt tggcgaaaga tcaaccaatg ccgtgtttat
    15421 ccacagaatg tgccactaag ttaagcactg aaccactaaa aactggagtt tcgtcgcacg
    15481 tcaaggctgt aaatgaaaac agtagtggag gtttttcaca gttatcccag ctttctgtgg
    15541 ataacatggt gtaagatcct gtttattttc agtgaccaga tttggaaaac ctattgcagt
    15601 gttgcgcaac tcgattaccg gcagcctaaa aagcaatata aatcagagaa ttgaacaaca
    15661 catgtggaaa aagttacact gcgaatattc ggcacataat tgctgtttgt tttttaacca
    15721 aggtatcatg acatgtccca acctcgccca ctgctctctc ctcccgaaac tgaagaacag
    15781 ttgttagcgc aagcacagca actttctggt tatacattgg gagaactggc ggcacttgac
    15841 gggctggtta cgccagagaa tttaaaacgc gataagggct ggattggcgt gttactggag
    15901 atctggctag gtgccagcgc agggagtaaa cctgagcaag attttgctgc tctgggcgtg
    15961 gaacttaaaa ctatccctgt ggatagtctt ggtcgtccgc tggaaacaac attcgtttgt
    16021 gttgcccggt taacgggtaa tagcggggtg acctgggaaa ccagccatgt gcgccacaag
    16081 ctcaaacgcg tactgtggat accggttgaa ggcgagcgca gcatcccgct ggcgcagcgt
    16141 cgcgtaggat caccgttgct gtggagcccg aatgaagagg aagaccgaca gctacgcgaa
    16201 gactgggaag aattaatgga tatgattgtt ctcggtcagg ttgagcggat caccgctcgt
    16261 cacggggaat atttacagat aagaccgaaa gcagcgaatg cgaaagcgct taccgaagcc
    16321 attggtgccc ggggcgaacg gattctgacg ctgccacgcg gcttttattt gaagaagaat
    16381 ttcaccagtg cactactggc ccgtcatttt ctgatccagt agccatcgct ttgacctgcc
    16441 gctttccggg catataatta ccgcttcatt tttttggcag ggctttttag atgttatttg
    16501 catggataac cgatcctaac gcttggcttg cgctcggtac gctgacgctg ctggagatcg
    16561 ttcttgggat cgacaatatt attttccttt ctctggtggt ggcaaagctt cccacagcac
    16621 aacgtgctca tgcgcgccgt ctggggttgg cgggagccat ggttatgcgt ctggcgctgc
    16681 tggcatcaat ctcttgggtt acgcgcctga caaatccgct ttttacaata ttcagtcagg
    16741 aaatttctgc ccgtgatttg attctgcttc tgggtggctt gttccttatc tggaaagcca
    16801 gcaaggaaat ccacgaatcc attgaaggtg aagaagaagg gctgaaaaca cgcgtttcat
    16861 cattcctcgg cgctatcgtg cagattatgc tgctggatat tatcttcagc ctcgactcgg
    16921 tgattaccgc tgtgggtctg tcagatcacc tgtttattat gatggcagcc gtggtgattg
    16981 ccgtaggcgt gatgatgttc gccgcgcgct cgattggtga ttttgtcgaa cgccatcctt
    17041 cggtaaaaat gctggcgctc tctttcctga ttctggtggg ctttaccctg attctggaaa
    17101 gtttcgacat ccacgtaccg aaaggttaca tctacttcgc gatgttcttc tctattgcgg
    17161 ttgaaagcct caacttgatt cgcaacaaaa agaatccgct ctgatactcc gtacgctctc
    17221 ctgcgggagg gcgtactcac tccttttacc ctcacagttt aagattttcc tgctttcaag
    17281 atatataacg tcggtttata aacagactat tatcataggt aggccaaatg aggataaccg
    17341 gatgaaaaaa tgggcagtaa taatttctgc agtcggactg gcgtttgctg tttccgggtg
    17401 ttccagtgat tacgtcatgg cgaccaaaga tggccgtatg attttgaccg atggaaaacc
    17461 tgaaattgat gatgataccg ggctggtgag ttatcacgat cagcaaggta acgcgatgca
    17521 aattaaccgt gatgatgttt cgcaaattat tgaacgttaa caaataaggt cagcatccgg
    17581 ctggccttaa gatttttctc tttccctttt cccttccctc tgccattttt atattcctta
    17641 tgtcgtgatt ataaaaagga aacggctatg caatatcacc gtatacccca cagttcgctg
    17701 gaagtcagca cgctggggct tggcacgatg acgtttggtg aacagaacag cgaagccgac
    17761 gcccacgcac aactcgacta tgccgtcgct cagggcatta accttatcga cgttgccgaa
    17821 atgtacccag tacctccgcg ccccgaaacg caagggttaa ccgaaaccta cgtcggcaac
    17881 tggctggcga aacatggcag ccgcgaaaag ttaattatcg cctccaaagt gagcggaccg
    17941 tcgcgcaata atgacaaggg catccgcccg gatcaggcgc tggatcggaa gaatatccgc
    18001 gaagcgctgc atgacagcct caagcgccta cagactgatt acctcgatct ttatcaggtg
    18061 cactggccgc agcgcccgac caactgcttc ggcaaactcg gttatagctg gacggattct
    18121 gcgcctgcgg tttcgctgct ggatacgctg gacgcactgg cagagtacca acgcgcggga
    18181 aaaattcgtt atatcggcgt gtcgaacgaa actgcatttg gcgtaatgcg ctacctgcat
    18241 ctggcggaca aacacgatct gccgcgtatt gtcaccattc agaaccctta cagtctgtta
    18301 aaccgcagtt ttgaagtagg tctggcagaa gtcagccagt atgaaggggt cgaactgctg
    18361 gcctattcgt gcctgggttt cggcacgctg accgggaaat atctcaatgg tgcaaaaccc
    18421 gctggcgcac gtaatacgct ctttagtcgg ttcacccgct atagcggtga gcaaacgcaa
    18481 aaagccgtcg cggcgtatgt tgatatcgcc agacgtcatg gccttgatcc tgctcagatg
    18541 gcgctcgcgt ttgtacgccg tcaaccgttt gttgccagca ctctgctggg cgcaaccacg
    18601 atggatcagc tgaaaactaa catcgaaagt ttgcatctgg agttaagcga agacgtatta
    18661 gctgaaattg aagcggtaca tcaggtttat acttatcctg caccataaag attaacgccg
    18721 gttaaaaccg gcgttaaata ttaatgacgg cgctgccaga tccacagcgc cgttattgcc
    18781 agcgcaaata gcgcaccgaa gccaatgcca atgggcacga ccgggatgcc taccattacc
    18841 gccagcgagt aaatgcccag catcaacaac atggcgctgt tttcgccaag gttttgtact
    18901 gcaatcgcat tccccgcccc gacgcttttt ttaccccgct cctgtagcaa cgcattgagc
    18961 ggaacgacaa aaaagccccc cagcacgcca atcagcatca acaaggcata ggctggcagc
    19021 agctcgtgtt gcagggagaa aatcggtacc accacgccaa tcaaaatccc ggctggcata
    19081 cagcgtgaca cggtttccag cgtaaccagc ttcgctgccg cacctgcgcc aaccacgata
    19141 ccaatcgcta ccatcgcgtt gagataggtc ggcgtagcgt tatcggtaat gcccagcgcc
    19201 accggtaccc acagcaccaa caggaaacgc agcgtgacac ccgctcccca gaataaactg
    19261 gtgcccacca gcgaaaaacg cgtttcacca ttgcaccata gcgaggtgca ggcattcagg
    19321 aaactgcggg tcatgttgat gagattccag gactgccccg gacgcgccgc cagtttggga
    19381 atgtagatat tggcaacgac cgcaccaccg taggccagtg cgcatgcggc cagagcgacc
    19441 aggacatgcc agtcagccag cacaccaccg gctacggaac cgagtaaaat cgccgctatg
    19501 gcagaagctt ccattaaacc gttagctttt actaacttac tacccgtggt taattcgccg
    19561 agaataccgt atttcgccgg tgaataggct gcagcaccaa cacccaccag cgtatagccg
    19621 agaaacggat tgataccaaa gcagatactg gctgcgccca gcagcttcag gccgttggca
    19681 aacatcatca cccggccttt ggcgaagcta tccgccacct gcccgacaaa cggcgccaaa
    19741 agaatgtaag cacctacaaa caccatttgc aggatgggct ggctccactc cggatagaac
    19801 tgcgctttca gtaacgccag agtggcaaac agtagcgcgt tatcgccaaa cgcagagaga
    19861 aactgcgcca cgataaccgc tttcatcccc ttcgaccaca acgaagtgtt agtgtgcact
    19921 gactcactca tcgtgttgtt ccgcttcgtc tacccagctt ttcaacgtga caaagtcagg
    19981 tttgccgctg ccaagtaatg gcatctgttt cagatagcga atatcgcgcg gtacagcaag
    20041 ctccggcacg ccgtgctcgc gggcatactg ttgcaactta tcgcgcgtca gttcgttatc
    20101 tgtggtgaaa agcaccagtg cctcgccttt gctggcatcg ctcttaatcg cagtggcatg
    20161 gactttatct ggcgaaacac caagtgccag ttgttccacc atttccagcg acaccatttc
    20221 gcctgcaatt ttggcaaagc gttttgcgcg gccctgaatc tgcacaaagc cctgctcgtc
    20281 aaaacgcaca atatcgccag tgtcatacca gccgcgctcc atttcgccgc gaacattctc
    20341 ggcggtgggc acttccagta cacctggctt ctccacccgc agatagccgt tcattatgtt
    20401 cggccctttc agttgcaggc gtccgccctc ttcgatacca gggaccgaca acaggcgtgc
    20461 atccattcct ggtagaatac gccctaccgt accgggtttc gccgccatcg gtacgttgat
    20521 agaaacgaca ggcgcgcatt cggtcacacc gtagccttca aggatgcgca ggccaaattt
    20581 atcctgccaa agctgtttgg tactttcttg tagtttttct gcgcctgcca ccacatagcg
    20641 tagacgatag aagtcatacg ggttggcgaa acgcgcgtag tgaccgagga aagtcgaggt
    20701 accgaacaac acggtgcaac tgcggtcata caccaagtcc ggcacaatgc ggtaatgcag
    20761 cgggcttgga taaaggaaca cttctgcacc tgtaagcagt ggcgtaaaca ggcctaccgt
    20821 cagcccaaag gagtgaaaca gcggtaatgc cgacataaag cgatcgttgg tggtgaagtc
    20881 ggcaatcgtt ttaatctgct cgacgttcgc cagaatgctt ttatggctat ggacgacgcc
    20941 ttttggatgg ccttcagaac cggaggtaaa gaggatcagc gcctcttctt ccggctgctg
    21001 ttttacctgc gccaggcgcg gcatcagcaa atgagcgaag atccatactt tgtcggcagt
    21061 ggtgacatct gcttttaaat cttccagata gacccagcgc acctgagtaa gttgctccgg
    21121 cagatgccag agtttgcctt tatcgagaaa ctggcgggaa gtgaagatgg ttttgatttc
    21181 agccgccgta atagcactgg tcagcccttt taccccggca gtgtagttca tcattgcggg
    21241 aatgcggcga cgcgcgatgg ccccaaaaat cactgccgca ctgatgcctg cattgggcag
    21301 cattaagccg atgcgttcgc cttcaacact gtatttttca aggatgcgtc caacaaacag
    21361 cgttttcgta agcaatttgc gataggagtc tggggtaaag ttgacgtctt cgacacattt
    21421 cttcccggct ccgaagcggt acattgcact cagtaaagat tcgtacagag tttcacgcgg
    21481 gcgcaccgcc atacgcgctt ccatcattat ttgatgcagc atttcgccag cgattttgcg
    21541 acggtcacgg gcacgcggcg catccggcat cgccacctgc gttggaggca aaatatgcag
    21601 agtaatttgc gggaacaagc gacgtttaac cagacctttc aggcggctga agtgcgtaag
    21661 ttccgcccct tcaatacgca caggaataac cgttgcacca gacttcgccg cgacaaaacc
    21721 cgcgccatcg tagattttca tcagcgagcc tgtagtggtg atgcgtcctt cagggaaaat
    21781 caccactggt cggccctgtt ccaccagacg taccagatgt ttaatagcca taggttgcgt
    21841 cgggtcgaga ggaacaaagt cgataaatga tttcagccaa cgcatatacc actgttggct
    21901 tattgaggtg taaacggcaa acactggacg cacaggtaaa aacagtccaa gcaaaatgcc
    21961 atcaataaaa gagacgtgat taggcgtaat tagtacgcgc tcgcccttca gtgcctgggt
    22021 gtcacccgta acgcgaacgc gatacaaaac acggcacaaa tttcgaaaaa agctaaaaag
    22081 cattccaact ccctttgctc tgattcagta aaagcgaatg gagggagatt acacgagata
    22141 aagaacgcga gcgacagtaa attaggtgcg aaaaaaaacc tgcgcatccg cgcaggttgg
    22201 tgcaagagac agggtacgaa gagcgtaccg aataatctca ccaatcaata cctctgggat
    22261 cttgattgtg gtctgcacga cgactcttcg ccagcgagaa aacgcaaagg aatgaaggga
    22321 aatgcaacga ggtgtgtaaa ttgtcggtta ctgttacaga ttgatgaccg gcaaaaaaaa
    22381 acctgcgcat ctgcgcaggc tggtgtaatt catgtgctca acacgaagtt gacttcacct
    22441 atcaatacct ctgggatcac cactttagca acctgaagcc aaacgccacc agcggtcaat
    22501 cgcaacagcg tttcgcaaag tgtaaccaaa ggtttgcaat ctcttttttt gtcttactta
    22561 tttgcgtttg gctcacattc ccacgatgaa aacacgccac cccttgaacc aacgggcgtt
    22621 ttccgtaaca ctgaaacaat gtaagcgttt acccactaag gtattttcat ggcgaccata
    22681 aaggatgtag cccgactggc aggcgtttca gtcgccaccg tttcccgcgt cattaataat
    22741 tcacccaaag ccagcgaagc ttcccggctg gctgtgcata gtgcaatgga gtctcttagc
    22801 tatcacccga acgccaacgc ccgtgcgctg gcgcagcaga ccactgaaac ggtcggtctg
    22861 atcgttggtg atgtttccga tccgtttttc ggtgcaatgg tgaaagcggt cgaacaggtg
    22921 gcttatcaca ccggtaattt cttattgatt ggcaacggtt accacaacga acaaaaagag
    22981 cgtcaggcta ttgagcaact gatccgccat cgctgtgctg cgttggtcgt ccatgccaaa
    23041 atgatcccag atgccgattt agcctcatta atgaaacaaa tgcccggtat ggtgctgatc
    23101 aaccgtatcc tgcctggctt tgaaaaccgt tgtattgctc ttgacgatcg ttacggtgcc
    23161 tggctggcaa cgcgtcattt aattcagcaa ggtcataccc gcattggtta tctgtgctct
    23221 aaccactcta tttctgacgc cgaagatcgt ctgcaagggt attacgatgc ccttgctgaa
    23281 agtggtattc cggccaatga tcggctggtg acatttggcg aaccggacga aagcggcggc
    23341 gaacaggcaa tgaccgagct tttgggacga ggaagaaatt tcactgcggt agcctgttat
    23401 aacgattcaa tggcggcggg cgcgatgggc gttctcaatg ataatggtat tgatgtaccg
    23461 ggtgagattt cgttaattgg ctttgatgat gtgctggtgt cacgctatgt gcgtccgcgc
    23521 ctgaccaccg tgcgttaccc aatcgtaacg atggcgaccc aggctgccga actggctttg
    23581 gcactggcgg ataatcgccc tctcccggaa attactaatg tatttagtcc gacgctggta
    23641 cgtcgtcatt cagtgtcaac tccgtcgctg gaggcaagtc atcatgcaac cagcgactaa
    23701 ccgcagttaa agcaattcca gcgccagtaa ttcttcgatg gtctggcgac ggcgaatcaa
    23761 ccgcgcctga ccattatcaa acagaacttc tggtaacagc ggacggctat tgtagttgga
    23821 tgacattgat gcgccatatg cccctgtatc atgcagtacc agataatcac ctgccttcac
    23881 ttccggcaag gcgcgggttt caacatttcc cccttcctgc tgggtaaaga catcgcccga
    23941 ttcacataac ggcccggcga cgacggtttc caccgttggc gcgtgttcca gagaacgacc
    24001 atcagctgcc agggcactga tatggtggta actaccgtac attgccgggc gcatcagatc
    24061 gttgaacccg gcatcaacca gcacaaagtg gcggctaccc atttgtttga cgctccgcac
    24121 ctgagtaatt aatacgccag actgcgctac caggaagcga cccggttcaa tttccagttt
    24181 cacagggtgg cccaaatggc gagcgatttg ctcacgcgcg gcattccaca gaccataata
    24241 atgttcggta tcaaccgcct cttcaccctg ttgataagga atagaaagcc caccgcccgc
    24301 agaaatagcc tgtaaatcct gaccgaattc gataacctga cgcaccatag caccacacac
    24361 ctgctccaga tgggcataat caacgccaga accaatgtgc atgtgaatgc cgactagctg
    24421 cagatgatga cgttgtatca cgtccagtgc ggcgggcaga tcggtgtacc agataccgtg
    24481 cttgctgttt tcgccaccgg tattggtttt ttggctatgt ccgtgaccaa accccggatt
    24541 aacgcgcagc catacccgat gccctggcga aacctggccc agttggtcga gcgtatcaac
    24601 agaacccgca ttcaccggaa tttgcaattc actgacgcgt tcaagcgtcg cctgatcgat
    24661 aacatctgcc gtaaaaacaa tatcatcggg gtgcgtttgc ggattgtaac ccgccgccaa
    24721 cgcacgctct atttcgccta acgagacgga atccactttc acgccctgct cacgcattaa
    24781 gcgcaaaata tgaatattgg aacaggcttt ctgtgcaaag cgcaccactt caaactgttt
    24841 cagcgctgca atctgccgac gaataatttg cgcatcgtag acccacaccg ggcagccaaa
    24901 ttcagcgggc aaacgcagca gattttcggc ggtgagatcg gtatcggtgc tgaacagtga
    24961 atgtggcata acaaactcca gatgagtgct tttttatgat tacgccacat cataaaaaga
    25021 ataaaaaata tcgatttatg tcgagtctat gcaaaaatga tatggattac cggattgcga
    25081 gagagcgcta atggccgccg ttaacttacg tcatattgaa atttttcatg cggtaatgac
    25141 cgccggaagc ctgactgagg cagcacacct gctacacacc tcacagccaa ccgtcagccg
    25201 cgaactggcg cgctttgaga aggtgatcgg gctgaaattg tttgagcgcg tacgtggacg
    25261 attacatcct accgtgcaag gactgcgtct gtttgaagaa gtgcaacgat cctggtacgg
    25321 actggatcgc attgtcagcg ccgcagaaag tctgcgcgag tttcgccagg gagaactgtc
    25381 tattgcctgc ctgccggtct tttcgcaatc ttttttaccg cagctcctgc aaccctttct
    25441 ggcacgttat cccgatgtca gcttaaatat cgtgccccag gaatcaccgc tacttgaaga
    25501 gtggctctcg gcccagcgtc atgatttagg actcactgag acactccata cgcctacggg
    25561 aacagaacgt accgaattac tctctttaga tgaagtgtgt gtgttacctc cgggtcatcc
    25621 gctggcggta aaaaaggtat taacgccgga tgattttcag ggtgagaatt acatcagcct
    25681 ttcccgtact gacagctatc gccagttgct ggatcagcta tttactgaac atcaggttaa
    25741 acgacgcatg atcgtagaaa cccacagcgc cgcgtcagtc tgcgcaatgg tacgggcggg
    25801 ggtaggtatt tcggtggtta acccgctcac cgcactagat tatgcggcaa gcggtttagt
    25861 ggtgcggcgg ttcagtattg cggttccgtt caccgtcagc ctgatccgcc ccctgcaccg
    25921 cccgtcatca gcgctggttc aggcgtttag tgggcattta caagcggggt taccgaaact
    25981 ggtcacttct cttgacgcta ttttgtcgtc agctacgaca gcataaaagc gacagcatcc
    26041 tcggcatgga tcgccgcggt atcaaacaca ggcaaaacac tgcgctcttc tggcaccagt
    26101 aaaccaattt ctgtgcagcc aaaaatgacg ccctgtgcgc cctgttctgc aaggcgagca
    26161 atcacttgcg cataataagc gcgtgacgct tcggtaaatt gccccagaca cagttcttca
    26221 aaaataatct gattaatttt cgcccgttca tccgcttcag gaataagaca attgatggaa
    26281 aattgttccg tcagccgccc gcgataaaaa tcctgttcca tggtgtaacg cgtacccagc
    26341 agcgccacac gagtcattcc ggccccggta attgcacgtc cggtggcatc cgcaatgtgt
    26401 aagaaaggca gagtgcaacg tgactcaatg gcatccgcca ctttatgcat cgtattggta
    26461 cataacacaa taccttctgc gcccgcccgc tgtaagccaa gcgccgcctc agccagaatg
    26521 tccccggttt tatcccattc cccgcgacgc tggcactctt ctatttcatg aaaatcgacg
    26581 ctatgtagca gcatttgcgc agagtgaagc ccaccaagcc gctgtttaat gccttcattt
    26641 atcagacgat agtaaggaat ggtggattcc cagctcattc ctcccagcaa accaattgtt
    26701 ttcattcctc tctcctgatg tgtgttaccg caacaaggct gaataaggaa cgagacaaac
    26761 gcctcaacgg ccaggtgccc aatctctatt aacgaaaaaa gggccggatg tacagcacat
    26821 ccggcccgtg aaatcagacg ccgatatttc tcaacttctc gcctgccatc agtttgcgtt
    26881 cgatatgttc cagcgtgaca tttttggttt ccggaatgag ccagaaagta atacccacaa
    26941 acgcaatgtt cagcgcagtg tagagccaga acgtaccggc agcgccaatg ctatcaagca
    27001 gtgtcaggaa ggccgcgccg ataatcatat tcgacaccca gtttgtcgtc gtcgaacagg
    27061 taataccgaa atcgcggcat ttcagcggct gaatttcaga gcacaggatc cacaccactg
    27121 gcgcggcgct catcgcataa ccggcaatac acatcatcgt catgccaaca gagagccagg
    27181 acaagccact ggaagccgta ccgttatcaa actgcatcag gcaatagccc agcaccagag
    27241 tgcctaacgc catcacgctg aaaccaattt tcagagccgg tttacgacct gctttatcta
    27301 ccgtaaagac cgcaatgaag gtcgcgaaca taaaggtcag tcccacgacc agagtcgcaa
    27361 tcatctgttg ttctgtggtc gtaaagcccg ccattttgaa gatacgcggc gcgtagtaca
    27421 tgatgatgtt cataccggta aactgctgca tcgcctgcaa caacataccg agaaacacag
    27481 cacgacggac gttacggttg atcttaaaca gtgcccaacc gccctgtttt aacttcaggc
    27541 tttcacgaat ttcgttgagt tcttctcgcg ctttttccga cgtatcgcgc agcatacgca
    27601 acacttcttc cgcctcaata tgacgcccct tttccgccag ccagcgcggg ctatttggca
    27661 ggaagaccac cagaataatc agcagaactg ctggtaaagc aagaaccccc aacattgcgc
    27721 gccagttacc gctataactg aacgctgtat cggataaaaa cgccagcacg atgccgagtg
    27781 tgaccatcaa ctggtacata ctgatcatct taccgcgaac gttttcactt gccatttcag
    27841 aaagatacag aggagcggtg taagacgcga tcccgacagc aatgcccagc accacacgag
    27901 cggcgattaa catctctacg ctggtcgcaa aagcggaccc tatagaaccg agtacaaaca
    27961 ggatggcccc cgccatcagg ctgtatttac gccccaggcg gaacgacagc caaccattaa
    28021 acagcgcacc aattgctgcg ccgagcatca tgctactaac cacccattcc tgcaaacgac
    28081 tggtcagcac aaagtgatcg gtaatgaacg gcaacgctcc ggcgattacg ccgatatcaa
    28141 gaccaaataa caatcctgcg accgcagcag ctaccgaaac aaacatattc atacgccgcg
    28201 tatcacgcaa agaacgtggc gttaaagcag attccgtatt gatagtaacc attttttcct
    28261 gccagcagag agtaagacat tgtgaaaaaa tacgtgaaca attcacacag gtgtcaggtc
    28321 ggaaacagca taaatatgga ttaaattgct gcgacatgcc gttatgtgat ggatattcca
    28381 attttaaaat taagttgaat tattgagatt attattaacc tcctaatttt aaagcagata
    28441 aaattcataa agttcattaa ttgataattg atatggatta tttcataacc atgatatgga
    28501 ttatgatgat ctagagatat aaaaaaccct gccatgcggc agggtcataa aagtaagagg
    28561 aatgaattaa cgcgccagcc aaccgccatc cacagcaatg gtataaccat ttacataatc
    28621 tgaagcgctg gaggcaagaa acactaccgg ccccatcagg tcactcggca gtccccaacg
    28681 gccagctgga atgcgatcga gaatttccgc gctacgttgt tcatctgccc gcagttgttg
    28741 ggtattgttg gtcgccatgt aacccggagc tatcgcatta acattaatgt tgtgtttagc
    28801 ccattcgttt gccatcaatc gcgtcacacc catcacgccg ctttttgatg cggtataaga
    28861 aggcacacgg atcccgccct ggaaggagag cattgacgcg atattgataa tcttgccgcc
    28921 attgccttgc gcgataaagt gtttcgccgc tgcctgagac atgaagaata cgctcttgat
    28981 attcaggttc atgacatcgt cccagtcttt ttcgctgaac tcgagagcgt cttcgcggcg
    29041 aatcaatccg gcgttattca ccaggatatc aatatgacca aattcagcta ccgcgcgatc
    29101 cagcagtcca ggaatgccat caatctttcg cagatcggcg gtcaggctta aaaaacgacg
    29161 ccccagcgcc gtgacctgct cgatggtttc agtcggttca acgatgttaa tgccaacaat
    29221 gtcacagccc gcttgcgcca gccccaacgc catcccctgg cccagcccag tatcacaacc
    29281 agtgacgacc gcaactttac cttggagaga aaatgcactt aaaatcataa caacacctta
    29341 ttcgtttatg cccacaacta gcgcaaatct ttaacgccca catggtccat atcattaaag
    29401 acctggtttt caccgaccat gccccagata aaggtataag ctttggttcc gacgccggaa
    29461 tggatcgacc agctcgggga gatcaccgcc tgctcgttat gcatcacaat gtgacgcgtt
    29521 tcttgcggct gccccatcat gtggaaaacg caggcgtcat catccatatt gaaatagaaa
    29581 taaacttcca tccggcgctc gtgggtgtga cacggcatgg tgttccacaa gttacctgga
    29641 gccagctccg tcagccccat actcaattga caggtttcca gtacatccgg gacaaaatat
    29701 ttgttaatcg tgcgacggtt actggtgagg ttatcgccta acgtgactgg agatacttcg
    29761 tccggtgtga cttttttggt gggatacgtc gtatgcgcgg gtgcgcaatt gtaataaaac
    29821 ttcgccggag tggcggtatc aatactggca aagacaactt cttttgcacc tttaccaaca
    29881 tacagggcgt cgcggtgacc gatttcatag cattgaccat cgacagtaat cgtaccggca
    29941 ccgccaatat tgataacacc taactcgcga cgttcaagga aatagcttac gccgagttgt
    30001 ttaccaactt ccccgccaac ggaaaccgtt ttagttatcg gcataatgcc gccaacaata
    30061 attcggtcaa tgtggctgta aaccatggtg tactcatcgg cgacaaatac cttttcaacc
    30121 aaaaattcat tgcgcagccc ttgggtatcc agcgtttttg cgtgcgcacc gtggatgctc
    30181 tgtcttacgt ccacatcaac ctccgataat cagtgaagcg tgaaaataga aaacgatagt
    30241 gaaacactgt ttcgttttct ctataaaggg atgatatgcc catgggctag ggttttcaat
    30301 taaaaataaa acaatgtttt atttttaatt taaggattaa aaaaagtgca gtgtcgatca
    30361 cgaatatgtc atgagaatgg aaaaatgagg gagcccagaa aatgtatgtc gacttgccag
    30421 ccatcattat tcaacagatt aatgacggca gatatttatc ggtaaggagg aaatgcagag
    30481 ctactcgtca cgttcaatgg tcaatgccac gccctggccc ccgccgatac aaagcgttgc
    30541 cagccctttg cgggcattac gtttcaccat ttcatgaacc agagaaacca ggattcggca
    30601 accggaagcg cctatcgggt gaccgagtgc gatcgcgcca ccattgacat tgacccgacg
    30661 ctcatcccac tcaagcatct tgccaaccga aagcgcctgt gcagcaaacg cttcattagc
    30721 ctcgataaga tcgacatcag ccaactgcca gcctacacgc tccaggcaac ggcgggtcgc
    30781 atacaccggc gcaattccca tcaatgccgg atctacacca acgctggcaa atgcgcggat
    30841 gcgggccagc acgggtaaat tcaacgctcg tgctttggct tcgctcatca tcattaccgc
    30901 agctgcgcca tcgtttatgg atgatgcatt ccccgcggtc acagaaccga gactatcaaa
    30961 tgaaggattt aaacgggcta aaccttctgc actggcgtca gtgcgtggct gttcatcggt
    31021 atcaacaacc aacgtctgac cgttactttg gatcattacc gggacgatct catctttaaa
    31081 tcgtccggcg tcaatcgccg ctcgcgcttt ttgttgcgag ctaagtgcgt aagcatcctg
    31141 caactgacgg ctgatgccat attcgcgagc cagattttcg gcggtgacac caatatgata
    31201 atcattgaag gcatcccaca acccatcatg caccagactg tcaaccaact ggctattgcc
    31261 aagctgttca ccggtgcggc tatcagtcag aacatgtggt gcgcggctca tgttttcctg
    31321 gccacctgcg atgacaatat cagcctcgcc acactgtatc gcctgagtag ccagatgcag
    31381 tgctttaaga ccggaaccgc aaacgtcatt aatcgtgatt gcagaaacgc tgttaggaag
    31441 accaccttta atagccgatt gccttgccgg attctgccct gcccctgcag tcaacacctg
    31501 accaagaatt acttcatcca ccgcatatgc aggaacgccg gtacgttcta ttaacgcttt
    31561 cacgaccaga ctaccaagtt ccacggcgga atgacccgct aacgcaccac gaaagcagcc
    31621 gataggtgtc cgtaacgccc cgacaatcac aacgtctttc atcacaacct cgcaccaatt
    31681 aacagtgcaa tagtaaatga ttgttaatta cagttatctt aattgtttaa aaaaagtgat
    31741 ttttatcaca aaggaaatat gcctgagcag tagtcagagt cataactggc acgtaaggtt
    31801 tgcaaccact aacccaccaa tagatgggta gatagggcgt taatctccca tacttaactt
    31861 ggtttatggt aaattgccct ccattttgtt taatttgtag atgatacgtt cagataatgt
    31921 ctaatatttg gtctaaagaa gaaactctgt gaagtttcgc gctctatggc acagccgttg
    31981 atgcaggcac gctattcctt cctattcagt taggttcggc aggggctgtg gtcctgttta
    32041 ttactgctct ggtcgcctgg cctttaacat attggccaca taaagcctta tgccagttca
    32101 tcctctcatc aaaaacatca gcaggtgaag ggataacggg cgcggtaaca cactactatg
    32161 gcaagaagat tggtaatctg attaccacgc tgtacttcat cgcctttttt gtcgtcgtgt
    32221 tgatatatgc agtggcaatt accaactcac ttacggaaca gctggcaaag catatggtta
    32281 ttgatcttcg catccgtatg ttggtgagtc tgggtgttgt attaattctg aatctcattt
    32341 ttctgatggg acgtcatgcc actattcggg taatgggatt tttggtattc ccattgattg
    32401 cctatttctt atttctttcc atttaccttg tcggtagttg gcaacctgat ctattaacaa
    32461 cccaggtaga gttcaatcag aatacccttc accagatatg gatatcgatt cccgtgatgg
    32521 ttttcgcctt tagccatacg cccattattt ctacgtttgc catagacaga cgtgaaaaat
    32581 atggcgaaca cgctatggat aaatgcaaaa aaattatgaa agtcgcttat ctcatcatct
    32641 gcataagtgt actgttcttt gtctttagct gcctgctttc tattccatct tcgtatattg
    32701 aagcggctaa agaagaaggg gtcaccattt tatcggcgct ttctatgctg ccgaacgccc
    32761 cagcatggtt gtcaatttcc gggattattg tcgcagtagt tgcgatgtcg aaatcattcc
    32821 tgggtacgta ctttggcgtt attgaaggtg ccacagaggt cgtcaaaaca acactacagc
    32881 aggttggtgt aaagaaaagt cgtgcattta accacgcact atcaattatg ttggtatcgc
    32941 tgattacctt cattgtttgt tgcattaacc cgaacgcgat ttcgatgatt tacgcgatca
    33001 gcggcccgct cattgccatg atacttttca tcatgcctac gctgtcaacg tatctcatcc
    33061 cggcgcttaa accctggcgt tccatcggaa atctgattac gctgatcgtg ggtatcctgt
    33121 gcgtatcggt aatgttcttt agctaatatc cgttgtggat ggttcaaaat tgctacaatt
    33181 ttgaaccatt ccatttttca taatattcat ttgttattat tccgccctat cacttctctc
    33241 gtcatatcct agaattatca gttagttaaa cacacaaaga gatctgaaca tcttaaataa
    33301 gactatttaa gatgcataac ttagattcgc aagatatctt ctggtcattc aaaaacaatt
    33361 tgcgatatag atattctcat accatgagat agttatctgg accacgttcg accatgcaag
    33421 attaaatcag tgaaacataa tcatatttaa taacgagaaa tgcattttta aatatcttaa
    33481 tcagaattat agcaaataca gattaatcgc aatttttccc agcaaaatta caaaatcaag
    33541 gtgtattgac atcatcaatc accaatgaaa ttatgcaatt atatacggat agggaggttc
    33601 ttaacatggg gctttgtagt cgttataaaa gtcttacatg caatagttgc tcaatgcatt
    33661 gccaaataat gccagaagag tcaccgcgtt tgcagtactg cgcaaactcg t
//
LOCUS       DAJTSI010000030        31935 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000030 DAJTSI010000000
VERSION     DAJTSI010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31935
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.030"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1150)
                     /gene="setB"
                     /locus_tag="OO327_001274"
     CDS             complement(<1..1150)
                     /gene="setB"
                     /locus_tag="OO327_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR6017886.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIAT"
     gene            1518..2648
                     /gene="fruB"
                     /locus_tag="OO327_001275"
     CDS             1518..2648
                     /gene="fruB"
                     /locus_tag="OO327_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR6017887.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2648..3586
                     /gene="fruK"
                     /locus_tag="OO327_001276"
     CDS             2648..3586
                     /gene="fruK"
                     /locus_tag="OO327_001276"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR6017888.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3603..5294
                     /gene="fruA"
                     /locus_tag="OO327_001277"
     CDS             3603..5294
                     /gene="fruA"
                     /locus_tag="OO327_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR6017889.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5718..6659
                     /locus_tag="OO327_001278"
     CDS             5718..6659
                     /locus_tag="OO327_001278"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR6017890.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6647..7585
                     /gene="psuG"
                     /locus_tag="OO327_001279"
     CDS             6647..7585
                     /gene="psuG"
                     /locus_tag="OO327_001279"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR6017891.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7679..8929
                     /locus_tag="OO327_001280"
     CDS             7679..8929
                     /locus_tag="OO327_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6017892.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9000..9694)
                     /gene="yeiL"
                     /locus_tag="OO327_001281"
                     /pseudo
     CDS             complement(9000..9694)
                     /gene="yeiL"
                     /locus_tag="OO327_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9825..10766
                     /gene="rihB"
                     /locus_tag="OO327_001282"
     CDS             9825..10766
                     /gene="rihB"
                     /locus_tag="OO327_001282"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCR6017893.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10866..12116
                     /locus_tag="OO327_001283"
     CDS             10866..12116
                     /locus_tag="OO327_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6017894.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12172..13260)
                     /locus_tag="OO327_001284"
     CDS             complement(12172..13260)
                     /locus_tag="OO327_001284"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6017895.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="OO327_001285"
     CDS             complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="OO327_001285"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR6017896.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14194..15243)
                     /locus_tag="OO327_001286"
     CDS             complement(14194..15243)
                     /locus_tag="OO327_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR6017897.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15342..16223
                     /locus_tag="OO327_001287"
     CDS             15342..16223
                     /locus_tag="OO327_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6017898.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16429..17898
                     /gene="lysP"
                     /locus_tag="OO327_001288"
     CDS             16429..17898
                     /gene="lysP"
                     /locus_tag="OO327_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR6017899.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18190..20181
                     /gene="cirA"
                     /locus_tag="OO327_001289"
                     /pseudo
     CDS             18190..20181
                     /gene="cirA"
                     /locus_tag="OO327_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20213..21048)
                     /gene="yeiG"
                     /locus_tag="OO327_001290"
                     /pseudo
     CDS             complement(20213..21048)
                     /gene="yeiG"
                     /locus_tag="OO327_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21306..21974
                     /gene="folE"
                     /locus_tag="OO327_001291"
     CDS             21306..21974
                     /gene="folE"
                     /locus_tag="OO327_001291"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR6017900.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21991..23067
                     /locus_tag="OO327_001292"
     CDS             21991..23067
                     /locus_tag="OO327_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR6017901.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23209..24249
                     /gene="galS"
                     /locus_tag="OO327_001293"
     CDS             23209..24249
                     /gene="galS"
                     /locus_tag="OO327_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCR6017902.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKNVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24529..25527
                     /gene="mglB"
                     /locus_tag="OO327_001294"
     CDS             24529..25527
                     /gene="mglB"
                     /locus_tag="OO327_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR6017903.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25588..27108
                     /gene="mglA"
                     /locus_tag="OO327_001295"
     CDS             25588..27108
                     /gene="mglA"
                     /locus_tag="OO327_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR6017904.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27124..28134
                     /gene="mglC"
                     /locus_tag="OO327_001296"
     CDS             27124..28134
                     /gene="mglC"
                     /locus_tag="OO327_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR6017905.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28170..29383)
                     /gene="preA"
                     /locus_tag="OO327_001297"
                     /pseudo
     CDS             complement(28170..29383)
                     /gene="preA"
                     /locus_tag="OO327_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29377..30615)
                     /gene="preT"
                     /locus_tag="OO327_001298"
     CDS             complement(29377..30615)
                     /gene="preT"
                     /locus_tag="OO327_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCR6017906.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30809..31048)
                     /locus_tag="OO327_001299"
     CDS             complement(30809..31048)
                     /locus_tag="OO327_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR6017907.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31051..31770)
                     /gene="sanA"
                     /locus_tag="OO327_001300"
     CDS             complement(31051..31770)
                     /gene="sanA"
                     /locus_tag="OO327_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR6017908.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
BASE COUNT         7811 a         7753 c         8426 g         7945 t
ORIGIN      
        1 gagtggcgat aatcatcacc atcgcaaacc agaacacagc gtgataattc cagatctcgg
       61 cgacgatgcc cgccactgat cctgcgatga tccagcccac gcgcgacgtg ttggtataga
      121 gcgtggtggc tgaacctgcc tgaccgggca tcaaatcctg aaaatagagc atcccgatgc
      181 cgcccagaat gccaataaaa atagcattta gcagctgcaa gcccaacaga atggcaggtg
      241 aatgcgccat cagcattcct gcgtaaaaac agacgccacc cacggcagca acgcgcatta
      301 agaaacgctt acccagacgt ttggcgaaat atccggcaat caacatcgtc gggatttcca
      361 gcccggcggc ggtccccatc atcacaccgg ccagtttctc gggaagatgc agttcgttga
      421 taataaatag cggcatgttg atgatgtaca ggctgttcga gccccacatc aatgtacaaa
      481 tgacaaacag cagtagcgta tcgcgacggt tacggcgcgg cgcttcaacc gtgccggtcg
      541 ccagcggaag ctctttttgc atcgacggta aaaacagcca caccatcacg ccgcaaacaa
      601 taaacgctac cgctgcgctc agatacatta ccgtaaagct gaaacccatc gctaacgcat
      661 aagccagcgg tgggccaatg acccatgcca gtgaaacctg agcgcgtaaa aaagagctga
      721 acatcaccgc ctcacgtccg gttttgtcgg catgttcacg ggcaagggca aacatttgcg
      781 ggttagcggt cgagccaaag ctgctaagaa agacgccaac gaatagcaaa acaaagtagt
      841 tgcgattcca agcaaaaagg gtgcaggcca gcacgcctaa caggcagcaa aagacaatca
      901 gcgatttgcg atcgccgcgc ttatcagagc gcccagcgag aaactgactt accagaatcc
      961 caatgacagc gctgccggta aagaagaatc ccaccatcgc cggacgggca tgtacttcat
     1021 cggtaaggaa aatactgagt gtcggggttt gcagagcacc cgcaataccg gtgagaaagg
     1081 caacgattaa aaacgccgtc gaggtcaggt caaacgattt cgcgctggag actgcggggg
     1141 agttatgcat gctggtcgat cactggaaga ggaagacgcg gagtttacgc tgcttgtcag
     1201 aaaataaaca gacagcgaaa tcaaaagcgc cacgaataat caaaatgcca tttcactgtt
     1261 gctgattggc tgctgacgct gctgaaatga aattgctgat aacggatttt cccatcagca
     1321 attaggaaaa atgacaaaaa attgtgcagc acatcaaact tttgctcata actttacggc
     1381 tttccttgcg tgctgaaaca gaagtattat gctttcttga aacgtttcag cgcgatcttg
     1441 tctttaaccc taagctaggt tggcgctttt tttctcatag aggctgaatc gtttcaattc
     1501 agcaagagag gagaactatg ttccagttat ccgtacagga catccatccg ggcgaaaagg
     1561 ccggagacaa agaagaggcg attcgccagg tcgctgcggc gctggtgcag gccggtaatg
     1621 tagcagaagg ctacgtcaat ggcatgctgg cgcgcgaaca gcaaacctca acgttcctcg
     1681 gcaatggtat tgctattcca cacggcacta ccgacacccg cgatcaggtg ctgaaaaccg
     1741 gcgttcaggt atttcagttc ccggaaggcg tcacctgggg tgacggtcag gtagcgtacg
     1801 tggcaatcgg tattgctgcc agctcggata aacatctggg cctgctacgc cagctgaccc
     1861 acgtactgag cgatgattcc gttgctgaac aactgaagtc agcaacaaca gcagaagaac
     1921 ttcgcgcatt actgatgggc gaaaagcaga gtgagcagct gaagctcgac aacgaaatgc
     1981 tgacgctgga tatcgtcgcc agcgatctgc tgactcttca ggcgctgaac gctgcgcgtc
     2041 tgaaagaggc gagggcagtt gacgccactt tcgtcaccaa agccatcaat gaacaaccgc
     2101 tgaatctcgg acagggtatc tggctgagcg atagcgccga aggcaatctg cgtagtgcga
     2161 ttgcggtaag ccgtgcggca aatgcttttg atgtggacgg cgaaacggca gccatgctgg
     2221 tgagtgtggc gatgaatgac gatcagccca tcgcggttct taagcgtctc gctgatttgt
     2281 tgctcgacaa taaagctgac cgcttgctga aagcggatgc ggcaacgttg ctggcgctgc
     2341 tgaccagcga tgatgcgccg accgacgacg tgttaagcgc ggagtttgtg gtgcgcaatg
     2401 aacacggcct gcatgctcgt ccaggtacca tgctggtcaa taccattaaa caatttaaca
     2461 gtgatattac cgtgacaaac cttgatggca ccggcaaacc ggcaaacgga cgtagtctga
     2521 tgaaagttgt ggcacttggc gttaagaaag gtcatcgcct acgctttacc gcccagggtg
     2581 cagatgctga acaggcgctg aaagccattg gcgacgctat cgctgctggt cttggggagg
     2641 gcgcataatg agcagacgtg ttgctacgat cacccttaat ccggcttatg accttgttgg
     2701 tttctgcccg gaaattgaac gcggcgaagt gaacctggtg aaaaccaccg gtctgcatgc
     2761 ggcgggtaaa ggcatcaacg tggccaaagt attaaaagac ctgggaattg atgtcaccgt
     2821 tggcggcttc ctgggtaaag acaatcagga tggttttcag caactgttca gcgagctggg
     2881 cattgccaac cgtttccagg ttgtacaggg gcgcacccga attaacgtta agctgacgga
     2941 aaaagacggc gaagtgaccg acttcaactt ctcgggtttc gaagtcaccc ccgccgactg
     3001 ggaacgcttt gtgactgatt ctctgagctg gctcggtcag ttcgatatgg tctgtgtcag
     3061 cggaagctta ccgtcaggcg tcagcccgga agcgttcacc gactggatga ctcgcctgcg
     3121 tagtcagtgt ccttgcatta tctttgatag tagccgtgaa gcgttagtag caggtttgaa
     3181 agcggcaccg tggctggtga aacctaaccg ccgcgagttg gaaatctggg caggccgtaa
     3241 actgcctgaa atgaaagatg tgattgaagc tgcacatgcg ctacgtgaac aaggtattgc
     3301 gcatgttgtt atttcactgg gtgccgaagg cgcgctttgg gttaatgcct ccggcgaatg
     3361 gatcgctaaa ccaccgtcag tcgatgtcgt aagcaccgtt ggcgcagggg attctatggt
     3421 tggtggcctg atttatggct tgctgatgcg tgaatccagt gaacacacac tgcgtctggc
     3481 gacagctgtt gcagccctgg cggtaagtca aagcaatgtg ggtattaccg atcgtccgca
     3541 gttggccgca atgatggcgc gcgtcgactt acaacctttt aactgacagc aggagaggca
     3601 taatgaaaac gctgctgatt attgacgcta atctcggtca ggcacgcgcc tatatggcga
     3661 agaccctgct gggcgcggcg gcgcgaaaag caaaactgga aatcatcgac aatccgaacg
     3721 acgcagaaat ggcgattgtt ctcggtgatt ccatcccgaa tgacagcgcg ctgaacggta
     3781 aaaatgtctg gctgggtgat atttcccggg cagttgcgca ccctgagctg ttcctgagtg
     3841 aagccaaagg ccatgcgaaa ccttacactg cgccggtcac tgcgacagca ccggttgccg
     3901 ccagcggtcc gaaacgcgta gttgcggtga ctgcttgccc gactggcgta gcacacacct
     3961 ttatggcggc tgaagccatt gaaaccgaag cgaaaaaacg tggctggtgg gtgaaagttg
     4021 aaacccgtgg ttctgttggc gcgggtaatg caatcactcc cgaggaagta gccgcagcgg
     4081 atctggtgat tgtggcggca gatatcgaag tggatctggc gaaatttgct ggtaaaccga
     4141 tgtatcgtac ctctaccggt ctggcgctga agaaaacctc gcaggaactg gataaagcgg
     4201 ttgctgaagc aacgccgtat gaaccggcgg gcaaagctca aacggcgacc actgaaggta
     4261 agaaagagag tgcaggcgct tatcgtcact tgctaacggg cgtctcttac atgctgccga
     4321 tggtcgttgc tggtggtctg tgtatcgcgc tttcttttgc ttttggtatc gaagcgttta
     4381 aagagccggg tacgttggca gcggcgctga tgcagattgg tggtggttca gcctttgcgc
     4441 tgatggtgcc ggtactggca ggttatattg ccttctccat tgccgatcgt ccgggcctca
     4501 cgccgggtct gattggcggt atgctggcgg tcagcaccgg ttctggcttc attggcggta
     4561 ttattgcggg cttcctggct ggttacattg cgaagttaat cagtacgcaa ctgaaactgc
     4621 cgcagagtat ggaggcgctg aaaccgatcc tgatcattcc gctaatttcc agtctggtgg
     4681 tcggtctggc gatgatctac ctgatcggta aaccagttgc tggcattctc gaagggctga
     4741 ctcactggct gcagaccatg gggactgcga atgcggttct gctgggggcg atcctcggtg
     4801 gcatgatgtg tactgacatg ggcggtccgg taaacaaagc agcgtacgca ttcggtgtgg
     4861 gtctgctgag tactcaaacc tatggcccga tggcggcgat tatggcggca ggtatggtgc
     4921 caccgctggc aatgggcctg gcaacaatgg tggcgcgtcg caaattcgac aaagcgcagc
     4981 aggaaggtgg caaagccgct ctggtattgg gactgtgctt catttcggaa ggtgcaattc
     5041 cgtttgctgc tcgtgatccg atgcgtgtgc tgccgtgctg tatcgtgggt ggggcgctga
     5101 ctggcgcaat ctcaatggcg attggtgcga aactgatggc accgcacggt ggtctgtttg
     5161 ttctgctgat ccctggcgct attacgccgg tattgggtta cctggtagca attattgccg
     5221 gtacgctggt ggcgggtttg gcctatgcct tcctgaaacg tccggaagtg gacgcagtag
     5281 cgaaagcagc gtaataaaag gtgtgttaca gggcagaaat ttcctgccct gtaacatttg
     5341 atgagcgcaa agagaatgac cagcttgtga atgtaatttt atgaatttaa atggttttat
     5401 ctttcagtag aaacttcaaa cgttcatcaa acactaaggt tatttcctca agggcagatt
     5461 attttctgcc cttttttagt gttcctgttc tgtgcatccc gtcacaaatt cctcgctttt
     5521 gtaattcagg catttaatcg cattttgtga gcctgctcaa atctgcccat tttgcctctc
     5581 tggcggtaat ttactttacg taaaacatgc atactatgag cacatgttta aatatgaaca
     5641 catgtttaaa ccgtctttgc agtatctata ttcttgtgga ttgacagggt tggtaatttt
     5701 ttaccagagg acgtgctatg cgcgaaaagg attatgtcgt aattataggt tcggcgaata
     5761 ttgatgtcgc cggatattca catgaatcat taaattatgc ggattcaaat ccaggtaaaa
     5821 taaaatttac gcctggtgga gtagggcgca atattgcaca aaacctggcg ttgctgggta
     5881 acaaagcctg gctactgagc gccgtaggca gtgattttta tggtcaatcg ctgctaacgc
     5941 aaaccaatca atctggtgtt tatgtcgata aatgcctgat tgtgccggga gaaaatacgt
     6001 cgagttattt atcattactc gataataccg gtgaaatgct ggttgctata aatgacatga
     6061 atattagcaa cgctattaca gctgaatatc tcgcacagca ccgtgaattt attcagaggg
     6121 caaaggtcat tgtcgcggac tgtaatatca gtgaagaggc actggcatgg attctggata
     6181 atgccgccaa cgtacccgta tttgtcgatc cggtttcagc gtggaaatgt gtcaaagtgc
     6241 gcgaccgtct aaatcagaac cacactctga agccaaaccg ccttgaagcg gaaaccctga
     6301 gtgggattgc gctgtcaggg cgtgacgatg tggcaaaagt tgctgcctgg ttccatcaac
     6361 atggcctgaa ccgactggta ttgagcatgg gcggcgacgg cgtttattac agcgatatca
     6421 gcggtgaaaa tggctggtct gcgccgatca aaaccaatgt tattaatgtt accggagcgg
     6481 gcgatgccat gatggcggga cttgcttcgt gttgggtaga cggaatgccg tttgccgaat
     6541 ctgttcgttt cgcacaggga tgttcgtcaa tggcgctctc ctgtgaatac accaataacc
     6601 ccgatttatc gattgccaac gttatatcgt tagtggagaa cgcagaatgt ctgaattaaa
     6661 aatttcccct gaattattac aaatttctcc ggaagtgcag gacgctttaa aaaacaaaaa
     6721 accggttgtg gcgctggaat cgaccattat ttctcacggg atgccgttcc cacaaaatgc
     6781 ccagaccgca attgaagtag aagaaactat tcgtaaacag ggcgcagtac ctgccactat
     6841 cgccattatt ggcggcgtga tgaaagtggg tttaagcaaa gaaaaaattg aattactggg
     6901 tcgtgaaggg cataacgtga ccaaagttag tcgtcgcgat ttaccttttg ttgttgccgc
     6961 cggaaaaaat ggcgcaacca ctgtggcttc aacgatgatt attgcggcgc ttgccggaat
     7021 taaagtattt gccaccgggg gaattggtgg tgtgcatcgc ggggcggaac ataccttcga
     7081 tatttctgcc gatttgcaag aactggcaaa tactaatgtc accgttgttt gtgccggggc
     7141 gaaatctatt ctcgatttag gattaaccac tgagtattta gaaaccttcg gtgtgccgtt
     7201 aattggctat cagactaaag cgctgcctgc gtttttctgc cgtaccagct cgtttgacgt
     7261 cagcattcgt ctcgacagcg ccagtgaaat tgcccgtgca atggcggtga aatggcaaag
     7321 cgggctgaac ggtggcctcg tggtagcgaa cccgatcccg gaacagtttg cgatgccgga
     7381 ggaatctatc aatgcagcca tagatcaagc cgtcgccgaa gccgaagagc agggcgttat
     7441 tggtaaagaa agtacaccgt tcctgctggc gcgcgttgct gagctgacca gcggtgacag
     7501 cctgaaatcc aatatccagc tggtgttcaa caacgccatt ctggcgagtg aaattgccaa
     7561 agaataccag cgtctcgcgg gttaatttct gacgggcaga aagccgtcgc ccgtactcat
     7621 caagcatacc aaaacctggc gctgacgccg ggtttactgg catgaaggga aaatcattat
     7681 ggatataatg agaagtgttg tggggatggt ggtgttactg gcaatagcat ttctgttgtc
     7741 agtgaataaa aagagcatca gtttgcgcac ggttggagcc gcactgctgc tgcaaatcgc
     7801 tattggtggc atcatgctct acttcccacc gggaaaatgg gcagtagaac aggcggcatt
     7861 aggcgttcat aaagtgatgt cttacagtga tgccggtagc gccttcattt ttggttcgct
     7921 ggttgggccg aaaatggatg tcctgtttga cggtgcgggt tttatcttcg cctttcgcgt
     7981 acttccggcg attattttcg ttactgcgct catcagtctg ctgtactaca ttggcgtgat
     8041 ggggctgctg attcgcatcc ttggcagcat tttccagaaa gccctcaaca tcagcaaaat
     8101 cgaatctttt gttgcggtta ccactatttt cctcgggcaa aatgagatcc cggcgatcgt
     8161 taaaccgttt atcgatcgcg tgaatcgcaa cgagttgttt accgcaattt gtagcgggat
     8221 ggcgtccatt gctggttcgg tgatgattgg ttatgccgga atgggcgtac caattgacta
     8281 cctgttagcg gcatcgctga tggcgatccc cggcggtatt ttgtttgcac gtattcttag
     8341 cccggcaacc gagccttcgc aggtcacatt tgaaaatctg tcgttcagcg aaacgccgcc
     8401 aaaaagcttt atcgaagcgg cggcgagcgg tgcgatgacc gggctgaaaa tcgccgctgg
     8461 tgtagcgacg gtggtaatgg cgtttgtcgc aattattgcg ctgatcaacg gcattatcgg
     8521 cggaattggc ggctggtttg gtttcgccaa tgcctctctg gaaagtattt ttggctatgt
     8581 gctggcaccg ctggcgtgga tcatgggtgt ggactggagt gatgccaacc ttgctggtag
     8641 cctgattggg cagaaactgg cgattaacga attcgtcgct tacctgagtt tctccccata
     8701 cctgcaaacg ggcggcacgc tggaagtgaa aaccgtggcg attatatcct ttgcgctttg
     8761 tggttttgct aactttggtt ctatcggtgt tgtcgttggc gcattttcgg ctatttcgcc
     8821 aaaacgtgct ccagaaatcg cccagcttgg tttacgggcg ctggcagcag caacgctttc
     8881 caacctgatg agtgcgacta ttgccgggtt ctttattggt ctggcgtaat gctcgatttg
     8941 tagaccggat aaaacgtatt aagcgttgca tccggcaatc gcgctattgc aaataatttt
     9001 tactgcatca tcccggagaa tttattctcc gggtccatct ccagcgccag tcctgacaac
     9061 tgctttctgt ttttaattag atatcctttc ttttaattaa taaaccgtcg tgaatgaatt
     9121 gcgcgagaac atataaaaga tgtcgataag aaacgcctaa atactctgcc gcttgcgtat
     9181 gcttttcgtg ataaagatca ccttcttgtg agagtaatat aaatgctgcc aggcgattca
     9241 ctaacggaaa tgattgattc tgagttaaag aaacaatatt acgataattt ttatgactta
     9301 aggtgacgca aagttttcgt aaaaataggg tgtcgtttaa taacagcgga cggtaatgtt
     9361 tcatagggag cgcaaggcac caacactctt caatagcctg caccgcacgc ggttcatggt
     9421 ctttgtcgat taactcaatc tcgccaataa aacagggagc ggcaaagaaa tcgatcagcg
     9481 acacgcgacc attggcaagc gtggcgtaaa gcctggcgcg gcctcgcgtc agataaaaca
     9541 gccatgacgg ctgttgccct tcctgcacaa tgtagtcacg cgctaaaaaa tgaaacagcc
     9601 gcgtatcggc tgaaacatcc gttgaaaagc aatccttaaa cgcagattca ctcatcaatt
     9661 gctgcttaag gtcattattg tggatttctt tcacgatgtt tatccgctat gagatttctc
     9721 atatttagca tacgtacgct gttgttaaga ttcaatcatt ggcccggtcg aattttcttt
     9781 gctgtagaaa aaaataacgc aactggaaac agaggaaata aacaatggaa aagagaaaaa
     9841 ttattctgga ttgtgagccg ggtcatgatg atgctattgc aatgatgatg gcagcgaaac
     9901 atccggcaat agatttatta ggcatcacta ttgtagcggg taatcagacg cttgataaaa
     9961 cattaattaa tggcctgaat gtttgccaga aactggagat taatgttccg gtttatgcgg
    10021 ggatgccgca acccattatg cgtaaacaaa tcgttgccga taatattcac ggtgaaaccg
    10081 gactggatgg cccggtattc gagccgctga cccgccaggc agaaagtacc catgcggtga
    10141 aatatatcat cgataccctg atggcaagcg atggcgatat cactctggtg ccggttggtc
    10201 cgctttcaaa tatcgcggtg gcaatgcgta tgcaacccgc aatcctgccc aaaatccgtg
    10261 aaattgttct aatgggcggc gcttacggta caggcaactt caccccatct gccgagttca
    10321 atatctttgc cgacccggaa gccgcccgcg tggtgttcac ctccggcgtt ccattagtga
    10381 tgatgggcct cgatcttacc aaccagaccg tttgcacccc ggatgtaatt gctcggatgg
    10441 aaagggtagg cggccctgcc ggagagctgt tcagcgacat catgaacttc actctcaaaa
    10501 cgcagttcga atactacggc ctggctggcg gcccggtgca tgacgccacc tgcattggtt
    10561 atctgatcaa ccctgatggc attaaaaccc aggatatgta cgtcgaagtg gacgtcaaca
    10621 gcggcccttg ctacgggcgt acggtctgcg acgaactggg cgttcttggc aagcccgcta
    10681 ataccaaagt cggcatcact attgataccg actggttctg gggcttagtc gaagagtgcg
    10741 tgcgcggcta catcaaaacc cattaagtgt tgataacaag ccgggacagt atcgcaagat
    10801 gcaggcccgg ctatttctga tgggcagaaa gccgtcgccc aaaatgacat gaagagataa
    10861 tcactatgga tgtcatgaga agtgttctgg gaatggtggt actgctggca attgcgtttt
    10921 tactgtcagt aaacaagaag aagatcagcc tgcgtaccgt tggcgcggcg ttagtgttac
    10981 aggtcgtgat tggcgggatt atgctttggt taccgccagg gcgttgggtc gcagaaaaag
    11041 tcgcttttgg cgtccacaaa gtgatggcgt acagcgacgc aggtagcgca tttatcttcg
    11101 gttctctggt cggaccgaaa atggatacct tatttgatgg cgcaggattt atctttggtt
    11161 tcagggtatt accggcaatt atcttcgtca ctgcactggt gagtattctc tactacatcg
    11221 gtgtgatggg gatcttaatt cgcattctcg gcggtatctt ccagaaagct ttaaatatca
    11281 gcaagatcga gtcattcgtc gcggtcacca ccattttcct cgggcaaaac gaaattccgg
    11341 cgatcgtcaa accctttatc gatcgtctga atcgcaatga attatttaca gcgatttgta
    11401 gtggcatggc ctcgattgct ggttcgacaa tgattggtta cgccgcactg ggcgtacctg
    11461 tggaatattt gctggcggca tcgttaatgg cgatccctgg cgggatcttg tttgcccgcc
    11521 tgttaagccc ggctacggaa tcttcgcagg tttcttttaa taacctctct ttcaccgaaa
    11581 caccgccaaa aagcattatt gaagccgatg cgacaggggc aatgaccggg ctgaaaatcg
    11641 ccgccggtgt agcgacagtt gttatggcat ttgtcgccat cattgcgtta attaacggta
    11701 ttatcggcgg cgttggcggc tggtttggtt ttgaacatgc ctcgctggag tccattttag
    11761 gttacctgct ggccccactt gcgtgggtaa tgggtgttga ctggagtgat gcaaatcttg
    11821 ccgggagttt gattggacag aaactggcaa taaatgaatt tgtcgcttat ctcaatttct
    11881 caccctatct gcaaacggct ggcactctgg atgctaaaac cgtggcgatt atttccttcg
    11941 cgttgtgcgg tttcgctaac tttggttcta tcggggtggt ggtgggggcg ttttctgcgg
    12001 ttgcgccaca ccgtgcgccg gaaatcgccc agcttggttt acgcgcgctg gcggcggcga
    12061 cactttctaa tctgatgagt gccaccattg ccgggttctt tattggttta gcgtagtgtg
    12121 caatttatga gccggattag atgcggtcaa gcgtcgaatc cggcaacatt gttacaccat
    12181 tggcactaat gacagcgcgt tatcggcaga cagggtaggg ttgtttaagc tgccgctggc
    12241 gcgtgaaatt gccgcgcagg ccatcgcaaa acgggcgctg tcacggaaat tgtttccctc
    12301 cagaaagcta tataccagac ccgccataaa accatcgtca gcaccaaaac tgtctaccgt
    12361 cgtatgcgct ggcacagtca gcaaaaattg ttcaccatcc ttttcgctgc aataaactga
    12421 ctcatcgggc aaataaacaa acagttgctg aacaccttgc tgatgcaatg cattcactgc
    12481 ggcattacgg tcagcatcgc tggtgatcgc ctgtccccat aaaatttcca gctccggtaa
    12541 agtgggtttc agggtgtgaa tatgcgccag ccagtgtttg attttgcccg ctttgaattc
    12601 tgaaacggta tcgacaaaca ccgggatctc atcagcgagg gtaaagaccc attccagcgc
    12661 ctcggctgtc aggttgcaat cagccagtac cacgcccgca tgacgaagta aatcgcgcga
    12721 cccgttcagt agttgcggtg tcaactgttc cagcagatgg gtatcgttaa ttgccagcac
    12781 ggtttgatcg tctcgattgg caattgccag atacgtcgat gtgctttgac catgcaaacg
    12841 aacgcagccg gagacattca cgccggcacg gcgcgtttct tccaggagca tttcgccata
    12901 aaagtcatcg ccaatcactg aaagcaaatg gacgtcacgg cctaacagcg ccagattgtg
    12961 ggcgatattg cgtccaacgc cgcctgccga acaatgaatt gtaccgggat gagaagccgc
    13021 ttgcgggtaa tggatatccg ccatcccgcg aatatccata ttgattgtcc ccaccactac
    13081 gcagtattcc tgctcggtga gaatgtaacc tttgtcttta atccggcctt tgcgcattaa
    13141 atccataata tgcgccgcaa cgcgcgaacg actgatttgc agcatgtccg caatttcgtt
    13201 ctgctgaatc agcgggttac gccgtaaaat tgcaaggatc tccttttccc ggttatttat
    13261 cttcaggcta ccgctttttc agtttgttgc gctttcagcc aggcgatctc ttctgcccag
    13321 atatccgggt tgatggtttc aaggatcagc ggaatgccgt cgaaacggtc gttctgcata
    13381 atccagcgga acgcatcatg accgatattg ccttcaccga ggctatgatg gcggtcaacg
    13441 cggctgccaa aggtactttt cgcatcgtta aggtgcatcc cgcgcaaata cttaaagccg
    13501 acaatacggg caaaatcagc gaacgttttt tcgcattcgg ctggagtacg caaatcatac
    13561 ccggcagcga aagcatggca ggtatcaatg cagacgccga cgcgggattt atcttccacg
    13621 ccgtcgataa tcgccgcgag atgttcgaat ttaaacccta agttactgcc ctgaccggcg
    13681 gtgttttcaa tcaccgctgt tacaccttga gttttatcca gcgcaatgtt gatggattcg
    13741 gcaatacgcg caaggcaatc ctcttctgaa atctgcatca gatggctgcc agggtggaag
    13801 ttgagcaaag aaagccccag ttgttcgcaa cgctgcattt catctataaa ggcatcgcgc
    13861 gatttttcca gagcttcagt gaccggatgt ccgaggttga tcagataact gtcgtgggga
    13921 agaatttgcg ccgatgtgta gtgatatttt tcacaggcgg ctttgaattc atcgatggtt
    13981 tgcgtcgtga gcggtgcggc acgccactga cgttggtttt tggtgaacaa ggcaaacgcg
    14041 gttgcgtcga tttcggcggc gcgaattgcg gcatttgcca gaccgccagc agcactaacg
    14101 tgcgctccaa tgtatttcat gcgaggactc ctgttaaacc cgctggagga aaacggtaat
    14161 gatagcgggt taacaggagc aagaagtagt ggtttatgcg atgacgcttt gaatcacata
    14221 gttaatcgca ccaccaccaa caatcagcca ggcaaacagt accagtgcca tcagcagagg
    14281 tttcgcccca gcttttttca gcgcgctgac gtgagtggtc agacccagcg ccgccatcgc
    14341 cattgccagc aggaaggtat ccagcgttac cagcatgttc accacgctct gcggtaacag
    14401 gtggaacgag ttaaagatgg caactacgat gaacaagatg gcaaaccacg gaatagtgat
    14461 tttgcttttc tcgccgctgt tcgccccaga cagctgttta acacgcgccg ccagcaggat
    14521 gaggaacgga gccagcatca tcacgcgcag cattttggaa ataactgctg cgttttctgc
    14581 atccgggctg atggcatgac ctgccgccac cacctgcgcc acttcgtgca cagtagaacc
    14641 aatgtagata ccgaaagttt ccggactaaa ccattgagac atcagcggat atatcgccgg
    14701 gtagaggaaa atcgcgacgg tcccgaagat aacaacggtt gcaacagcca cggttacttt
    14761 actggcttcc gctttcacta ccggctcagt cgccagtacc gcggcagcac cacagatact
    14821 gctaccggca ccgatcaacc aactggtgtg cttatccaga ccaaacactt tctgccccag
    14881 gaagcaagcc agcaggaagg tactggacag cgtcagcacg tcaatgatga tcccactgat
    14941 accgacatcg gcaatttgcg agaacgtcag acggaagcca taaagaatga tacccagacg
    15001 taataaatat tgcttggcaa acagcacacc accgtcacag cttttccaga tgtgcggata
    15061 gatggtgttg cctaaaacca tccccaacaa gattgcgagg gtgagggcac taaacccggc
    15121 acccgcaacc gcgggaatgg aaccacccca cagggcgacc ccggtgataa ctgcactcag
    15181 ggctaacccc ggaataaaat gccacagtgt acgatgttgt ttctgtaagg tgatattcgt
    15241 cataaccctc tcctttaacc gggcataagg ttacgactaa ctggtttaaa aataaaattg
    15301 attatatatt tataattaat ctttataagt ggtaagcgac tatgcacatc accctccggc
    15361 agttggaagt ttttgcagaa gtattgaaaa gtggatcaac cacccaggcg tcggtgatgc
    15421 tggcgttgtc gcaatcagca gtgagcgcag ccttgactga cctggaaggg cagcttggcg
    15481 ttcaactgtt tgatcgcgtg gggaaaagac tggttgttaa tgaacacggg cggctgctct
    15541 atccgcgagc gttggcactg cttgaacagg cggttgaaat cgaacaactg tttcgcgaag
    15601 acaacggcgc gattcgtatc tatgccagta gtaccatcgg taactacatt ctgcctgcag
    15661 ttatcgcccg ttatcgccat gattatccgc agttgccgat tgaacttagc gttgggaaca
    15721 gccaggacgt gatgcaagcg gtgctggatt tccgcgttga tattggcttt attgaaggac
    15781 cgtgccacag cactgaaatc atttctgaac cgtggctgga agacgagctg gtggttttcg
    15841 ccgcgccgac ttcgccgttg gctcgtggtc cggtcacctt agaacagctg gccgctgcgc
    15901 cgtggatcct gcgtgaacgc ggttccggca cgcgggatat tgtcgattat ctgttgctgt
    15961 cacatttacc gaagtttgag atggcgatgg aattaggtaa ctccgaggca atcaaacatg
    16021 cggtgcgtca tgggttggga attagttgcc tgtcgcgacg tgtgattgaa gatcaattgc
    16081 aggcaggcac attaagtgaa gttgcggtcc ctctgccgcg cctgatgcgt acgttgtggc
    16141 gtatacatca tcggcaaaaa cacctttcca acgcgctacg gcgctttctg gactattgcg
    16201 atcccgcaaa tgtgccgcgt taagttgctg tacaagaaca tgctggtgct gtgtcgattt
    16261 cgtgacgcag cgccttcagc atgcattcgc cagaaaagag attggctgct ttacttataa
    16321 tccctgggcg atcatgaagg tgtcttataa ccgtgtattt ctgccggaag gattgccaat
    16381 cgtctgctac aatcgcgcca tcatttttaa gatggatagc atttttgtat gggttccgaa
    16441 actaaaacta cagaagcgcc gggcttacgc cgtgaattaa aggcgcgtca cctgacgatg
    16501 attgccattg gcggttccat cggtacaggt ctttttgttg cctctggcgc aacgatttct
    16561 caggcaggtc cgggcggggc attgctctcg tatatgctga ttggcctgat ggtttacttc
    16621 ctgatgacca gtctcggtga attggctgca tatatgccgg tttccggttc gtttgccact
    16681 tacggtcaga actatgttga agaaggcttt ggcttcgcgc tgggctggaa ctactggtac
    16741 aactgggcgg tgactattgc cgttgacctg gttgcagcac agctggtcat gagctggtgg
    16801 ttcccggata caccgggctg gatctggagt gcgttgttcc tcggcgttat cttcctgttg
    16861 aactacatct ctgttcgcgg ctttggtgaa gcggaatact ggttctcact gatcaaagtc
    16921 acgacagtta ttgtctttat catcgttggt gtgctgatga ttatcggtat cttcaaaggc
    16981 gcgcagcctg cgggctggag caactggaca atcggcgaag cgccgtttgc tggtggtttt
    17041 gcggcgatga tcggcgtagc tatgattgtc ggcttctctt tccagggaac cgagctgatc
    17101 ggtattgctg caggcgagtc cgaagatccg gcgaaaaaca ttccacgcgc ggtacgtcag
    17161 gtattctggc gaatcctgtt gttctatgtg ttcgcgatcc tgattatcag cctgattatt
    17221 ccgtataccg atccgagcct gctgcgtaac gatgttaaag acatcagcgt cagtccgttc
    17281 accctggtgt tccagcacgc gggtctgctc tctgcggcgg cggtgatgaa cgcagttatt
    17341 ctgacggcgg tgctgtcagc aggtaactcc ggtatgtatg cgtctactcg tatgctgtac
    17401 accctggcgt gtgacggtaa agcgccgcgc attttcgcta aattgtcgcg tggtggcgtg
    17461 ccgcgtaatg cgctgtatgc gacgacggtg attgccggtt tgtgcttcct gacctccatg
    17521 tttggcaacc agacggtata cctgtggctg ctgaacacct ccgggatgac gggttttatc
    17581 gcctggctgg ggattgccat tagccactat cgcttccgtc gcggttacgt attgcaggga
    17641 cacgacatta acgatctgcc gtaccgttca ggtttcttcc cactggggcc gatcttcgca
    17701 ttcattctgt gtctgattat cactttgggc cagaactacg aagcgttcct gaaagatacc
    17761 attgactggg gcggcgtagc ggcaacgtat attggtatcc cgctgttcct gattatttgg
    17821 ttcggctaca agctgattaa aggaactcac ttcgtacgct acagcgaaat gaagttcccg
    17881 cagaacgata agaaataagt ttcctccctt ccttgctaag ccctctcaac cgagagggct
    17941 ttttcaattc catttccctg acaaatcatg cggatataaa atttaacatt tggattgata
    18001 attgttatcg tttgcattat cgttacgacg caatcaaaaa aggctgacaa atcagaggct
    18061 gttccggctt tctgggatga tcacctgcat acaaataagt ccaccgcgat gctgccgtac
    18121 gcaaggggac gtaaagaaga tgtgagcgat acccatttta ttttcgtagt tacctcatgg
    18181 agatatggaa tgtttaggtt gaaccctttc gtacgggtcg ggctgtgttt gtccgctatt
    18241 tcttgtgcat ggcctgtgtt agcggtcgat gatgatggcg aaacgatggt tgtcactgca
    18301 tcttccgtgg aacaaaatct taaagatgca cccgccagta tcagcgtcat tacccaggaa
    18361 gacctgcagc gaaaaccggt acagaatctg aaggatgtcc tcaaagaagt gcctggcgta
    18421 caactgacga acgaagggga taaccgtaag ggcgttagta ttcgtggtct ggacagcagc
    18481 tacaccctga ttcttgtcga cggtaaacgc gttaactccc gcaatgccgt cttccgccac
    18541 aatgatttcg atctgaactg gatcccggtc gattccatcg aacgtattga agtggttcgt
    18601 ggcccgatgt cgtcgctata cggctccgat gcgctcggcg gtgtagtgaa tatcatcacc
    18661 aaaaaaatcg gtcagaaatg gtcgggtacc gttaccgtcg ataccaccat tcaggaacat
    18721 cgcgatcgcg gtgataccta taacggtcag ttctttacca gtggaccatt aattgatggt
    18781 gtgctgggaa tgaaagctta cggcagcctg gcaaaacgtg aaaaggatga cccgcaaaac
    18841 tcaacgacca ccgataccgg agaaacgccg cgtattgaag gattctccag ccgcgacggc
    18901 aatgtcgaat ttgcctagac accgaatcaa aatcacgatt ttactgccgg atacggtttc
    18961 gaccgtcagg atcgtgattc cgattcgctg gacaaaaacc gcctggaacg ccagaactac
    19021 tgcgtcagcc ataatgggcg ttgggattac ggcaccagcg aactgaaatc ctacggtgag
    19081 aaagtcgaga acaaaaaccc tggcaacagc agcccgataa cttccgaaag caatacggtc
    19141 gacggcaaat acacgttgcc gctgacggcg attaatcagt ttctcacggt tggcggtgaa
    19201 tggcgtcacg acaaacttag cgatgcggtg aacctgaccg ggggaaccag ctccaaaacg
    19261 tctgccagcc agtacgcgct gtttgtggaa gatgaatggc ggatcttcga gccgctggcg
    19321 ctgacgaccg gcgtgcgtat ggacgatcac gaaacctacg gtgaacactg gagtccgcgt
    19381 gcctacatgg tttataacgc caccgacacc gtaacggtga aagggggctg ggcgacggca
    19441 tttaaagcac cttctctgtt gcaacttagc cctgactgga cgagcaattc ctgccgtggc
    19501 gcatgtaaga ttgtgggtag cccggatctg aaaccagaaa ccagcgaaag ttgggagctg
    19561 gggctttact acatgggtga agaaggctgg ctggaagggg gtgaatccag cgttatcgtt
    19621 ttccgtaacg atgtgaaaga tcgtatcagc atcagccgta cgtctgacgt caacgctgca
    19681 ccgggctacc aaaactttgt tggttttgag acgggcgcta acggacggcg cataccggta
    19741 tttagctact acagcgttaa caaagctcgt attcagggcg tggaaaccga actgaaaatt
    19801 ccgttcaacg atgaatggaa actgtcgatc aactacacct acaacgatgg tcgtgatgtc
    19861 agcaacggcg aaaacaaacc gctatccgat ctgccgttcc atactgctaa cggtacgctg
    19921 gactggaaac cgctggcgct ggaagactgg tcattctatg tttctggtca ctataccggg
    19981 cagaaacgcg ccgacagcgc gacggctaaa acaccgggcg gttataccat ctggaatacc
    20041 ggcgcggcct ggcaggtgac taaagacgtc aaactgcgcg caggcgtgct gaaccttggc
    20101 gacaaggatc tcagtcgtga cgactacagc tataacgaag acggacgtcg ttactttatg
    20161 gcagtggatt atcgcttctg atgagaagat gcccggcgaa ccgggcggac tttcacttca
    20221 gtaaatactg cgcatggaag cgcaggtgat cctctataaa agaggcgatg aagtagtaac
    20281 tgtgatcata tcccggctga atacgcagcg tcatcggcca cgctttctgg cgcgcggctt
    20341 ctgccaatac cgcaggttgc aactggtcgg caagaaactg atcattatcg ccctgatcga
    20401 taagcgtcgg gatcgcatcc tgcgcgttac tggcatacat cagcgcgcaa ctatcccatt
    20461 ccagcatgca tttttgtcct cacctaaata ggtgctaaac gctttgattc cccacgggac
    20521 gctgcacgga ttcacaatgg gcgcaaaggc cgaaacgctg gtgtatttac ccggattttt
    20581 cagcgccata atcagcgcac cgtgaccacc cattgagtga ccgctaatgg cgcagcggtc
    20641 gctgacatta aattgcgact gaaccagcgc cggtaattca tcgcgcagat aatcatacat
    20701 ccggtaatgc gtcgcccacg gcggttgcgt ggcattaaga taaaagcctg cgccctggcc
    20761 taaatcgtag ccatcgtcgt tggcaacctt ttcgccgcgc gggctggtgt ctggcatcac
    20821 cagtacaatc cccagttccg ccgctacccg ctgggcaccc gccttggtgg tgaagttctc
    20881 gtcattgcag gttaatccgg aaagccagta cagcactggt ggcggggtgt gatcacgagg
    20941 gggggggaga aagatactga acgtcatcgg gcagtttaag gtactggagt cgtgtcgcca
    21001 gcgttgctgc cagccttcaa aacagcggtg ctcttcgagc atttccatgc gtggctcctt
    21061 gttgtgttgt ttgcaatttc ttcatcatac cgtttattca atgtgctgtg agtaactttc
    21121 acttccgtat ttgcataacg atgttttaac atctgctgat gaaaggcagc ggcaattaca
    21181 ataattatcg ctgtgaatac tggattatgt gcgccgcctc acgcacaata atcaagctgt
    21241 aaatcagctt aataactttg cccccacgca gggtggaggc gtcacacctg caggagaaat
    21301 cataaatgcc atcactcagt aaagaagcgg ccctggttca tgaagcgtta gttgcgagag
    21361 gactggaaac accgctgcgc ccgcccgtgc atgaaatgga taacgaaacg cgcaaaagcc
    21421 ttattgctgg tcatatgacc gaaatcatgc agctgctgaa tctcgacctg gctgatgaca
    21481 gtttgatgga aacgccgcat cgcatcgcta aaatgtatgt cgatgaaatt ttctccggtc
    21541 tggattacgc caatttcccg aaaatcaccc tcattgaaaa caaaatgaag gtcgatgaaa
    21601 tggtcaccgt gcgcgatatc actctgacca gtacctgtga acaccatttt gttaccatcg
    21661 atggcaaagc gacggtggct tatatcccga aagattcggt gatcggtctg tcaaaaatta
    21721 accgtatcgt gcagttcttt gcccagcgtc cgcaggtgca ggaacgtctg acgcagcaaa
    21781 ttcttattgc gctacaaacg ctgctgggca ccaataacgt ggctgtctcg atcgacgcgg
    21841 tgcattactg cgtgaaggcg cgtggcatcc gcgatgcaac cagtgccacg acaacgacct
    21901 ctcttggtgg attgttcaaa tccagtcaga atacgcgcca cgagtttctg cgcgctgtgc
    21961 gccatcacaa ctgattaaaa ggcaggaacc atggagcgca acgtcacgct cgattttgtt
    22021 cgcggcgtcg ccattctggg gatcctgcta ttaaacatca gcgcctttgg gctaccaaag
    22081 gcggcttatc tcaatcccgc ctggtacgat gctattacgc cgcaggatgc atggacctgg
    22141 gcatttctcg atctcatcgg ccaggtgaaa ttcctcacgc tttttgcgct gctgtttggt
    22201 gctggcctgc aaatgttgct gccccgtggc agacgctgga tccagtcgcg gttaacgctg
    22261 ttagtcttgt tgggctttat tcacggttta ttgttctggg acggcgatat tctgctggct
    22321 tacgggctgg tggggcttgg cgttttgctg ttattggggc tgatttccga cagccagacc
    22381 agccgcgcct ggacgccgga tgcatcggct attttgtatg aaaaatactg gaagcttcac
    22441 ggcggcgttg aagcgatcag taatcgtgcc gatggtgttg gcaacagttt actggcactg
    22501 ggcgcacagt atggctggca actggcaggg atgatgctca ttggtgcggc gctgatgcgc
    22561 agcggctggc tgaaagggca gttcagctta cgtcactatc gtcgtactgg ttttgtgctg
    22621 gtggcgattg gggtgaccat taaccttcct gccatcgccc tgcaatggca gctggactgg
    22681 gcgtatcgct ggtgtgcctt cttacttcaa atgccgcggg aactgagtgc gccgtttcag
    22741 gcgattggct atgcgtcgct gttttatggc ttctggccgc aattgagccg ctttaagctg
    22801 gtgcttgcga tcgcctgcgt cggacggatg gcgctgacca actatctatt gcaaacgctg
    22861 atttgtacca cgctttttta ccacctcggt ttgtttatgc attttgaccg cctggagctg
    22921 ctggcgtttg ttattccggt atggctggcg aatatcctct tctctgttat ctggctgcgt
    22981 tacttccgcc aggggccggt ggaatggctc tggcgtcagt taactttgcg tgctgccgga
    23041 ccggcaatat ctaaaacatc aagataacga tctggattac attcgttaac aaaacggctg
    23101 taaccgtttc cattgctgtg actcgattca cgaagtcctg tattcagtgc tgacaaaata
    23161 gccgccagca agcagtcatt tactgcaatc tcataacagg tagtgaatat gatcaccatt
    23221 cgtgatgtag cgcgtcaggc tggcgtctct gtggcaacgg tttcccgggt gctcaataac
    23281 agcacgctgg tcagtgccga cacgcgtgaa gcagtaatga aagccgtgag tgagctggat
    23341 tatcggccaa acgccaatgc ccaggcgctg gcaactcagg ttagcgacac cattggcgtg
    23401 gtggtgatgg acgtttctga tgcgtttttc ggcgcgctgg taaaagcggt ggatctggtc
    23461 gcccagcagc atcagaaaaa cgtgctaatc ggcaatagct atcatgaagc ggaaaaagag
    23521 cgtcacgcca ttgaggtgtt aattcgccag cgttgtaatg cgttgattgt tcactcaaaa
    23581 gcattgagtg acgatgaact ggcgcaattt atggataaca ttcccggtat ggtgttaatc
    23641 aaccgcgttg tgccggggta cgcccatcgt tgcgtttgcc tggataatct cagcggtgcc
    23701 cgaatggcga cgcgcatgtt gctgaataac ggtcatcaac gtattggtta tctttcttcc
    23761 agccacggca ttgaagatga cgccatgcgt aaagcaggct ggatgagtgc gttgaaagag
    23821 caggatatta ttccgccgga aagctggatt ggcactggta cgccggacat gccgggcggt
    23881 gaggcggcga tggttgaact gctggggcgc aatctacaac ttaccgctgt atttgcttat
    23941 aacgacaata tggccgctgg cgcactgaca gcattaaaag ataatggcat tgcgattccg
    24001 ttacatctct caatcatcgg tttcgatgat attcccatcg cccgttacac cgacccgcaa
    24061 ttaacgaccg tgcgttatcc cattgcttca atggctaaat tagccaccga actggccttg
    24121 cagggggcag caggcaatat tgatcctcgt gccagccact gttttatgcc gacgttagtg
    24181 cgtcgccatt ctgtcgcaac gcgccagaat gcggcggcga tcactaactc aacaaatcag
    24241 gcgatgtaac cgctttcaat ctgtgagtga tttcacagta tcttaacaat gtgatagcta
    24301 tgattgcacc gttttaatgt tgtaacccgt atgtaacagt gaataatcac tttcaccgag
    24361 gtaatagcgt cataacaaca attaaagccg ttttctggag cgttaccggg catggaagaa
    24421 cgaattttaa aaagtgagct tcggcgttca gtaacacttc attaactcta ctgccccgcc
    24481 gagcatttat ctcaagcact accctgcata agaaaaaccg gagataccat gaataagaag
    24541 gtgttaaccc tgtctgctgt gatggccagc atgttattcg gtgccgctgc acacgctgct
    24601 gatactcgca ttggtgtaac aatctataag tacgacgata actttatgtc tgtagtgcgc
    24661 aaggctattg agcaagatgc gaaagccgcg ccagatgttc agctgctgat gaatgattct
    24721 cagaatgacc agtccaagca gaacgatcag atcgacgtat tgctggcgaa aggggtgaag
    24781 gcactggcaa tcaacctggt tgacccggca gctgcgggta cggtgattga gaaagcgcgt
    24841 gggcaaaacg tgccggtggt tttcttcaac aaagaaccgt ctcgtaaggc gctggatagc
    24901 tacgacaaag cctactacgt tggcactgac tccaaagagt ccggcattat tcagggcgat
    24961 ttgattgcta aacactgggc ggcgaatcag ggttgggatc tgaacaaaga cggtcagatt
    25021 cagttcgtac tgctgaaagg tgaaccgggc catccggatg cagaagcacg taccacttac
    25081 gtgattaaag aattgaacga taaaggcatc aaaactgaac agttacagtt agataccgca
    25141 atgtgggaca ccgctcaggc gaaagataag atggacgcct ggctgtctgg cccgaacgcc
    25201 aacaaaatcg aagtggttat cgccaacaac gatgcgatgg caatgggcgc ggtagaagca
    25261 ctgaaagcac acaacaagtc cagcattccg gtgtttggcg tcgatgcgct gccagaagcg
    25321 ctggcgctgg tgaaatccgg tgcactggcg ggcacagtac tgaacgatgc taacaaccag
    25381 gcgaaagcga cctttgatct ggcgaaaaac ctggccgatg gcaaaggtgc ggctgatggc
    25441 accaactgga aaatcgacaa caaagtggtc cgcgtacctt atgttggcgt agataaagac
    25501 aacttggctg aattcagcaa gaaataagac tgattgggcg cagtcgttta ctgcgccctt
    25561 attgtatggc caacaaggta aaattatatg gtcagctcaa cgactccgtc ttccggggaa
    25621 tacttgttgg aaatgagcgg tatcaacaag tcctttcctg gtgttaaggc acttgataac
    25681 gttaatttaa aagtccggcc acattctatc catgcattaa tgggggaaaa cggtgcagga
    25741 aaatcgacat tattaaaatg cctgtttggt atttatcaaa aagactccgg caccatttta
    25801 ttccagggta aagagatcga tttccattcc gccaaagagg cactggaaaa tggtatttcg
    25861 atggtacacc aggagttaaa cctggtatta caacgttcgg tgatggataa catgtggctg
    25921 gggcgatatc ccaccaaagg catgtttgtc gatcaggaca aaatgtaccg cgaaaccaaa
    25981 gcgatttttg atgaactgga tattgatatc gatccgcgtg cgcgcgtcgg cacattatcc
    26041 gtttcgcaaa tgcagatgat cgaaatcgcc aaagcgtttt cctataacgc gaaaattgtg
    26101 attatggatg aaccgacttc ttcgttaacc gaaaaagagg tcaatcatct gttcactatt
    26161 attcgtaaat taaaagagcg cggctgcggt attgtttata tctcgcataa aatggaagaa
    26221 atcttccagt tatgtgatga agttaccgta ttgcgcgacg gtcagtggat cgccaccgaa
    26281 ccgctggcag gactgacgat ggacaagatc atcgccatga tggttgggcg ttctcttaat
    26341 cagcgttttc ctgacaaaga aaacaagccg ggcgaagtca tcctcgaggt gcgtaaccta
    26401 acgtcactgc gccagccgtc gattcgcgat gtctcgtttg atctgcataa aggggagatc
    26461 ctcggtattg ccgggctggt gggggcgaaa cgtaccgata ttgttgagac gttatttggt
    26521 attcgcgaga aatcggctgg caccattacg ttgcacggca aacagatcaa taaccataat
    26581 gccaacgaag ccataaacca cggatttgca ctggtaactg aggagcgccg ctcaacggga
    26641 atttatgcct atctggatat tggttttaac tcgttaattt ccaatattcg caactacaaa
    26701 aataaagttg gtttactgga taactcgcgg atgaaaagcg atacccagtg ggtgattgat
    26761 tcgatgcggg taaaaacgcc gggtcatcgg acgcaaattg gttcgctctc cggtggtaat
    26821 cagcaaaagg tgattattgg tcgctggcta ctaacgcaac cagaaatatt aatgctcgat
    26881 gaaccgacgc gcggtattga tgtcggggcg aagtttgaaa tttatcagtt aattgccgaa
    26941 ctggcgaaga aaggcaaggg gattattatt atctcctctg aaatgcctga gttgttaggg
    27001 ataacagacc gtattctggt catgagcaat ggtctcgttt ccggaattgt cgatacaaaa
    27061 acaacaacgc aaaacgaaat tctgcgtctt gcgtctttgc acctttaaga ttaggggctt
    27121 cccatgagtg cgttaaataa gaaaagtttt cttacttacc tgaaagaggg cggtatttac
    27181 gtcgttcttt tagttttgct ggcgattatt attttccagg acccaacatt tttaagtctg
    27241 ttgaacttaa gtaatattct cacccagtca tcggtgcgta ttattatcgc gctcggtgtg
    27301 gcagggttaa ttgtcaccca ggggaccgat ctttctgctg gtcgtcaggt agggctggcg
    27361 gcagtggtgg ctgcgacatt attgcagtcc atggataacg ccaacaaagt gttcccggaa
    27421 atggcgacga tgccgattgc gctggttatt ctgattgtct gtgccattgg tgcggtgatc
    27481 ggtttgatca acggtctgat tatcgcttat ctcaacgtga cgccgttcat taccacgctc
    27541 ggcacgatga tcatcgtcta tggcatcaac tcgctctatt acgactttgt cggggcgtcg
    27601 ccaatttctg gttttgacag tggcttctct acctttgctc agggctttgt cgcgctgggg
    27661 agtttccgtc tctcttacat caccttctac gcgttgattg cggtggcgtt cgtctgggtg
    27721 ttgtggaaca aaacccgctt cggtaagaac atttttgcca ttggcggtaa cccggaagcg
    27781 gcaaaaatat ctggtgtcaa cgtcggcctg aacctgctga tgatctacgc gttgtctggc
    27841 gtgttctatg cctttggcgg gatgttagaa gccggacgta tcggctctgc caccaacaac
    27901 ctcggcttta tgtatgagct ggatgctatc gcggcgtgcg tggtaggcgg tgtatcgttc
    27961 agcggcggtg tggggacggt gattggcgtg gtgaccgggg taattatttt taccgtcatc
    28021 aactatggcc tgacgtatat cggcgtaaac ccatactggc agtacatcat caaaggggcg
    28081 attattatct tcgccgtagc gctggattca ctgaaatacg cgcgtaagaa gtgatgtgat
    28141 tgcggatatt gccggatgcg tctctggttt tacaacgtta ccgggtgttc tttctcgcct
    28201 ttcttaaact tcacttcccc gagctcaata caacccaccg ggcagacgtg accacaaagc
    28261 agacaaccca cacatttctc ggtattacaa tgcggtgtgc gggttttctc gctccattcc
    28321 atcgcctggt gaccgccgtc gtaacaggaa atataacagc gtccacagcc aacacatttg
    28381 tcaagattga tacggggata gacaatataa ctgcggtcta aatcttccgc cgggacaata
    28441 ttgttattcg ccagacctac catctcctgc aacgaatcaa atccctgatc ggcgagataa
    28501 tgcgataacc cgctcgccat atcttctacg atgcgatagc cgtactgcat aatgccggtg
    28561 gtgacctgta acgttgctgc gccgagcaat aaaaactccg ccgcatcttc ccaggtttca
    28621 atgccgccga taccgctgat tgggaaatcg cgcagttctg gatgggtgcg catttgctga
    28681 atgaagcgca gggcgatcgg ttttaccgct ttaccggaat atccggaaat actcgattta
    28741 ccgttaacga tcggcatacc gattttctga ttaagatcga tattggtgat ggatttaacg
    28801 gtgttaattg ccgcaatgcc atctgcgccg ccgcgcttcg ccgccagcgc cacttcgcac
    28861 atatcaccga tattcggcgt catcttcgcc agcattggca gcgtggaacc ccgtttcact
    28921 gcccgacaat atttttctac cagctccggg ctttgcccga catcgctacc catcgcatga
    28981 gaagtcattt gcggacagga gaagttacac tcgatcatat ccgcgccagc tgctcccatt
    29041 gctgctcatt ttcccccatg atcgaagcga tcaatacttt gtccgggtaa tcttccttca
    29101 gccgacgcag ggcggtcaga ttctcttcca acggatgttc agcaatctgc tccatatttt
    29161 tgaagccgat aaaaccggta tcttctttca ccagatgatc aaaacgcggc gagacttcgt
    29221 tggcgataaa aaagccgatc gttttaaaca ccacaccgcc ccaacctgtg tcgtaggctt
    29281 tggcacacat ctcatagcag ttgcctaccg gcgaagaaga gaggcagaac gggttgggaa
    29341 agttcacgcc gcaaaaagta atcgaaagat ctttcgttaa catgagcaag ctccctctaa
    29401 atagtgatga atcgcctcgg cggcttcttt cccggttttc acggcataga ccacggtttt
    29461 gtcaccctca acaatatcgc cagcagcaaa gacttgcgga tcgcggatct gataattttg
    29521 tgttttgatg gtgttacgct gctgctctaa ctccgcaaag gcatccagtc tggcatgctg
    29581 accgacggcg agaataattt tatctgccgc catcgtcagt tcgcctgata accgtacatg
    29641 cttaaacgtg actttattgc cttccacggc tactggcgtg aatccatcga tgatcgaaac
    29701 gcccagctcc cgggcgctgg caaactcttt ttcgcttgcc ggaaactcat ctaactcttc
    29761 acgtgctacg caagttaccg cctgacagcc gagaactttc agcgtgctgg ctacgtccat
    29821 cgcgacatca ccgccgccga taattaatgc gctttgtgga atgctgatat cgccttgcgc
    29881 ctgtcgtaca cgttgcagga agtcgacggc aatctcaacg tcgctatgct caaacagcgg
    29941 tagcccggaa ccgcttgata accccacggt gaccagtacc gcgcggtttt ccgctttcag
    30001 ttgctccagg gtgagtgttt taccgatttc gttattgcac ttaatggtca cgcccatttt
    30061 ttcaatacgg gcgatctctg catccagcac tgattgcggt aaccggaatt gcggaatacc
    30121 gttacgcaac cagccaccgg ggtgcgcttc tttctcataa atcgtgacgt cataaccctg
    30181 gtttgtcagt gtcacactgg cctgcaatcc ggcaggacct gcgccaataa tcgcgacttt
    30241 gccgagcgtt ttagtaccgg gctgataaat ttccatcccg gtttgttgtt caaaatcagt
    30301 aacaaaacgc tgtaagcggc cgatatcaat gggagcatca acaccggcac gggtacaacc
    30361 gctttggcat aatttctccg tcgggcacac tctggcacaa acggcaccga gggcattatt
    30421 ttcgcgaatt gtctcggcag cgcctttaaa attacgaaag tagattgagc gaataaattt
    30481 ccccggatcg gtctgtgccg ggcaagcctg actacaggga gcgtcgtgac ataataaaca
    30541 gcgagaggct tctttaatcg ccagtaacgg cgtaaaagcc ggagtgagtt catccagata
    30601 attttgttgc ggcatgtgat cttccttaat gctatgagta attatggcga acgctaaata
    30661 aatattgcgt gctgatatta aggctatgcg cgtggggaaa tttaagctgt gaaaggattc
    30721 acaaatagct gcggttcaga atgttatatc cgcagcttat tgagtgaagt aataattatt
    30781 ctcaataatt aacagaaaag agaaaatatt aacgatagaa aataagataa acaatcatgc
    30841 cgatagataa aattccgaag ccaatatatg ccaccatata agcgaagaag agtccgggat
    30901 tttttgctcg ccagacatac accggttccg gtgcattttt aacccgttta tcaatcgcgc
    30961 ctgcgcgcca gcctttccat gcttcgcgga aacaaaatat cgggataagg aaaaaaacgg
    31021 caacgacaaa aaactgctgt acatccataa ctactttcct tgtttctttt gtaattcaag
    31081 taactgttcg ggtgtaacgg cgggataccc ctgcgcatct tccggcacct ggtgcatagc
    31141 cggaataggg accagcggcc ctaaaaaacg cggttcacgt ttaaaaatat aaaggtcagc
    31201 cagcgcaccg aaacgggcgg caaattcgcg aatacgtact gacagcatat ctttcggtga
    31261 cggtacggca taacactgag cctgaatccc catatgcagc gcaataaata atgctcgctc
    31321 acagtggaaa cgttgggtga taataatgaa atcattagta tcgaaaactt tgcgtgtacg
    31381 cacgatggaa tccagcgtac gaaagcctgc gtaatcgaga acaatatctg atgggtcgac
    31441 accagcagcg attaaatctt tgcgcatggt catcggctcg ttataacttt gcaatgcgtt
    31501 atcgccgctc agtaatagat aatttacctt accgctgtta taggcattaa tcgctccttg
    31561 aatgcggtag cgataatact gattaataac gccggtacga taatattttg ctgtgccgag
    31621 caccacgccg acctggcggt aggggagatc ctgcaattcg tcgtagatat aaggcgcggt
    31681 tttccagctc atccagcgat cgaggccgag cacagtcaac agcagcaagc cgatcaggac
    31741 taacaggctg aggaacacgc gctttaacat gcaggagact cgagctaagg aaaatttcca
    31801 tcaggctact ttacccgctg gttttgcgca agaaacggca aactgtttgc cgggaatttc
    31861 gacaccagga gagggctggc aagccggaca aggcgttcac gccgcatccg gcaccaggct
    31921 taagcgagaa gcacg
//
LOCUS       DAJTSI010000031        31570 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000031 DAJTSI010000000
VERSION     DAJTSI010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31570
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.031"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..406)
                     /gene="trmJ"
                     /locus_tag="OO327_001301"
     CDS             complement(<1..406)
                     /gene="trmJ"
                     /locus_tag="OO327_001301"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR6017909.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIA"
     gene            525..1328
                     /gene="suhB"
                     /locus_tag="OO327_001302"
     CDS             525..1328
                     /gene="suhB"
                     /locus_tag="OO327_001302"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR6017910.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            1473..2327
                     /locus_tag="OO327_001303"
     CDS             1473..2327
                     /locus_tag="OO327_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6017911.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            2518..3798
                     /gene="csiE"
                     /locus_tag="OO327_001304"
     CDS             2518..3798
                     /gene="csiE"
                     /locus_tag="OO327_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR6017912.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(3790..4929)
                     /locus_tag="OO327_001305"
     CDS             complement(3790..4929)
                     /locus_tag="OO327_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR6017913.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(5089..5979)
                     /gene="hcaR"
                     /locus_tag="OO327_001306"
     CDS             complement(5089..5979)
                     /gene="hcaR"
                     /locus_tag="OO327_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCR6017914.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            6115..7476
                     /locus_tag="OO327_001307"
     CDS             6115..7476
                     /locus_tag="OO327_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR6017915.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            7473..7991
                     /locus_tag="OO327_001308"
     CDS             7473..7991
                     /locus_tag="OO327_001308"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR6017916.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            7991..8311
                     /locus_tag="OO327_001309"
     CDS             7991..8311
                     /locus_tag="OO327_001309"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR6017917.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            8308..9120
                     /gene="hcaB"
                     /locus_tag="OO327_001310"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             8308..9120
                     /gene="hcaB"
                     /locus_tag="OO327_001310"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR6017918.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            9130..10347
                     /locus_tag="OO327_001311"
     CDS             9130..10347
                     /locus_tag="OO327_001311"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HCR6017919.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRSAAQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVV
                     DGEKVELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIF
                     AGGDVAITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQ
                     FIGDMRGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDA
                     KLLIDENIALKSL"
     gene            10444..10866
                     /locus_tag="OO327_001312"
     CDS             10444..10866
                     /locus_tag="OO327_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6017920.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(10914..11786)
                     /locus_tag="OO327_001313"
     CDS             complement(10914..11786)
                     /locus_tag="OO327_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR6017921.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(11798..12892)
                     /locus_tag="OO327_001314"
     CDS             complement(11798..12892)
                     /locus_tag="OO327_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6017922.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(12925..13923)
                     /locus_tag="OO327_001315"
     CDS             complement(12925..13923)
                     /locus_tag="OO327_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6017923.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(13948..15459)
                     /locus_tag="OO327_001316"
     CDS             complement(13948..15459)
                     /locus_tag="OO327_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6017924.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEAMLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(15482..16464)
                     /locus_tag="OO327_001317"
                     /pseudo
     CDS             complement(15482..16464)
                     /locus_tag="OO327_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(16561..19662)
                     /locus_tag="OO327_001318"
     CDS             complement(16561..19662)
                     /locus_tag="OO327_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCR6017925.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWVNPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKKPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            complement(19669..19842)
                     /locus_tag="OO327_001319"
     CDS             complement(19669..19842)
                     /locus_tag="OO327_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6017926.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            19960..21153
                     /locus_tag="OO327_001320"
     CDS             19960..21153
                     /locus_tag="OO327_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR6017927.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(21351..22604)
                     /gene="glyA"
                     /locus_tag="OO327_001321"
     CDS             complement(21351..22604)
                     /gene="glyA"
                     /locus_tag="OO327_001321"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR6017928.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            22931..24121
                     /gene="hmpA"
                     /locus_tag="OO327_001322"
     CDS             22931..24121
                     /gene="hmpA"
                     /locus_tag="OO327_001322"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR6017929.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(24166..24504)
                     /gene="glnB"
                     /locus_tag="OO327_001323"
     CDS             complement(24166..24504)
                     /gene="glnB"
                     /locus_tag="OO327_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR6017930.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(24565..25899)
                     /gene="glrR"
                     /locus_tag="OO327_001324"
     CDS             complement(24565..25899)
                     /gene="glrR"
                     /locus_tag="OO327_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR6017931.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(25889..26602)
                     /gene="qseG"
                     /locus_tag="OO327_001325"
     CDS             complement(25889..26602)
                     /gene="qseG"
                     /locus_tag="OO327_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR6017932.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(26767..28194)
                     /gene="qseE"
                     /locus_tag="OO327_001326"
     CDS             complement(26767..28194)
                     /gene="qseE"
                     /locus_tag="OO327_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR6017933.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(28752..>31570)
                     /gene="purL"
                     /locus_tag="OO327_001327"
                     /gene_synonym="purI"
     CDS             complement(28752..>31570)
                     /gene="purL"
                     /locus_tag="OO327_001327"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR6017934.1"
                     /translation="RIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTYEEKVNSHNG
                     EELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGGGAASSMASG
                     QSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGLSNAMPELVS
                     DGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDELCKRERAPYA
                     VIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGDALVREGITI
                     ADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAVTTASLDSYY
                     GEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANWMAAAGHPGE
                     DAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPLSLVISAFAR
                     VEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPADVRDVVQLKG
                     FYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGDDRLAALFNE
                     ELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQTVFSESRTTL
                     RVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVAAPYIATGAR
                     PKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHALVACGGFSYG
                     DVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRELIPGSELWP
                     RFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVRDAAHLAALE
                     SKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPERVFRTVSNS
                     WHPENWGEDGPWMRIFRNARKQLG"
BASE COUNT         7539 a         8722 c         7990 g         7319 t
ORIGIN      
        1 ccgcaatcgc gacatgataa tggcatttct gcaactcttc attggtcaag ccgacgcgct
       61 cacgaccaaa caccagcgcc accggggtat ttgccgcttc agcgacgctt ttcagaccgc
      121 attcgcgcgg gtcgagcatc ggccacggca gcgtgcggga gcgtgcgctg gtgcccacca
      181 ccaggctaca accggctaac gcttcgtcga gcgtatcgac gatgtgagca ttaccaatca
      241 catcgctggc ccctgcagcc agggcaatcg cctgggagtc gggtttcacc agtggattaa
      301 ccagccacag attggttaat cccattgttt tcatggcacg ggcaacagaa cccatattgc
      361 cggtgtgtga cgtctccacc agcacaattc gaatattttg cagcattgtc tttcttcatc
      421 taaagattat tcacgcatct tatcataaaa cgaagacaga tgccgatctc gctgctatac
      481 tctgcgccgt tttcccgttc tttaacatcc agtgagagag accgatgcat ccgatgctga
      541 acatcgccgt gcgcgcagcg cgcaaggcgg gtaatttaat tgccaaaaac tatgaaaccc
      601 cggacgctgt agaagcgagc cagaaaggca gtaacgattt cgtgaccaac gtagataaag
      661 ctgccgaagc ggtgattatc gacacgattc gtaaatctta cccacagcac accatcatca
      721 ccgaagaaag cggtgaactt gaaggtactg atcaggatgt tcaatgggtt atcgatccac
      781 tggatggtac taccaacttt atcaaacgtc tgccgcactt cgcggtatct atcgccgttc
      841 gtatcaaagg ccgcaccgaa gttgctgtgg tatacgatcc tatgcgtaac gaactgttca
      901 ccgccactcg cggtcaaggc gcacagctga acggctaccg tctgcgcggt agcaccgctc
      961 gcgatctcga cggtaccatt ctggcgaccg gcttcccgtt caaagcaaaa cagtacgcca
     1021 ctacctacat caacatcgtc ggcaagctgt tcaacgaatg tgcagacttc cgtcgtaccg
     1081 gttctgcggc gctggatctg gcttacgtcg ctgcgggtcg tgttgacggt ttctttgaaa
     1141 tcggtctgcg tccgtgggat ttcgcggcag gcgagctgct ggttcgtgaa gcgggcggca
     1201 tcgtcagcga cttcaccggt ggtcataact atatgctgac cggtaacatc gttgctggta
     1261 acccgcgtgt tgtgaaagcc atactggcga acatgcgtga cgagttaagc gacgctctga
     1321 agcattaatg actcaggcgg gtgatatcac tcacccgccc tcgcctttca ggcgctattc
     1381 cgaaatactt cctcaccgct ttactttctt tcgtcactct cccaccatct ttccccgatt
     1441 aatggatgat aaagaagtcg caaggaaagg ttatggcact gccagtgaac aaacgcgttc
     1501 ccaaaattct gtttattctc tttgttgttg ccttctgcgt ttatttagtg ccgcgcgttg
     1561 ccatcaactt cttctattat cctgacgata aaatttacgg tcccgacccc tggtcggcgg
     1621 aatccgtcga atttacggct aaggacggta ctcgtctgca aggctggttt attccttctt
     1681 cgacgggccc tgctgacaac gccatcgcaa ccatcattca tgctcacggc aatgccggaa
     1741 atatgtccgc ccactggccg ctggtcagtt ggttacctga gcgtaatttc aacgttttta
     1801 tgtttgatta tcgcgggttt ggtaaatcaa aaggcactcc gtcccaggcc ggattgctgg
     1861 acgatacgca aagcgccatc aatgtggtgc gccatcgcag tgatgtaaac ccacaacagc
     1921 tggtgctgtt cgggcagagt attggcgggg cgaatattct tgctgttatt ggtcagggtg
     1981 atcgtgaagg catacgtgcg gtgatcctcg actccacatt tgcctcttat gcaaccatcg
     2041 ccaaccagat gatccccggc agtggctact tacttgatga gagttacagc ggcgaaaatt
     2101 acatcgccag cgtcagcccg atcccgcttt tactcattca cggtaaagcc gatcacgtta
     2161 tcccatggca gcacagcgaa aagttgtata gcctggcaaa agagccaaaa cggttgatcc
     2221 taatcccgga tggcgaacac attgatgctt tttccgatcg tcacggcaat gtttatcgcg
     2281 aacagatggt ggactttatc cttagcgcgt tgaatccgca gaactaaccc atgatcgcta
     2341 gcacgataat cattcacaaa accaccttaa gacatgctaa tccactggtc agaacagttt
     2401 aaaatgagaa aaattctgtg acgcttgcca acatttctga tgattagcat tcccttcgcc
     2461 atttccctga gcaaacttta gctattctta tcaattatgc ttatgggaga tctacagatg
     2521 atgcctacgc ttgctccacc atctgtcctt tcggctcccc agcgccgctg ccagatcttg
     2581 ctgacgctct ttcagccggg gttgaccgcc accatggcaa ccttcagcga gcttaatggt
     2641 gtggatgatg atattgccag tcttgatatc agcgaaacag gacgggagat cctgcgctat
     2701 catcaactca cactgacgac tggttatgac ggtagctacc gggttgaagg tacagtgctt
     2761 aaccaacgtt tgtgtttatt tcactggcta cgacgtggtt tccgtctgtg tccgtcattt
     2821 attaccagcc aattcacccc cgccctgaag agtgaactga agcggcgcgg aattgcgcgt
     2881 aacttttacg acgataccaa tctacaagcg ttagtgaatc tctgctcccg acggctgcaa
     2941 aaacgctttg aatcgcgcga tattcatttc ctgtgtctgt atctgcaata ttgtttgctg
     3001 cagcaccacg ctggaattac gcctcagttt aatccgctcc aacgtcgctg ggcggaatcc
     3061 tgccttgaat ttcaggtagc gcaggaaatt ggacgccact ggcagcgtcg ggcgctccag
     3121 catgtaccgc ctgatgagcc actgtttatg gcattacttt tttccatgtt gcgggttccc
     3181 gatccactgc gggatgcgca tcagcgggac agacaattgc gtcagtctat caaacgtctg
     3241 gtaaaccatt ttcgtgagct gggaaatgtt cgtttttatg atgaacaggg gttatgtgat
     3301 cagctttata tccacctcgc ccaggcgtta aatcgcagtt tgtttgccat cggtattgat
     3361 aataccctgc cggaagagtt cgccagactg tatccacgcc tggtacgcac cacccgcgcg
     3421 gcgctggccg gatttgaaag tgaatacggc gtccatcttt ctgatgagga aagtggtctg
     3481 gtcgcggtga ttttcggtgc ctggctaatg caggaaaacg atctgcatga aaaacagatt
     3541 attctactga ccgggaatga tagcgagcga gaagcgcaga ttgagcagca gttacgcgaa
     3601 ctaacgttac tgccgctcaa cattaagcat atgtcggtaa aggcattttt gcagacaggc
     3661 gctccgcgtg gcgcggcact gattattgcg ccttatacca tgccgttacc gctcttttca
     3721 ccaccgctga tctatacgga cctgacgctg acaacacatc aacaggagca gatccgcaaa
     3781 atgctcgaat cagcatgagg gaacaacttt cgggcgcaaa aacattgccg gaagcgccac
     3841 cagcgccatt acccagaaca cgccgtggcc cagatattga tacaggaaac cggcgaaaac
     3901 ggtcatgata gcgatactgc cgcccatcgc gacggcagag taaaccgcct gtaaacggat
     3961 gacttcgcta acctggcgag cagcaatata acgcatggcg gccaggtggc agaccgtgaa
     4021 ggtgccgcaa tgcagaattt gcaccactat caaccacggc aacgccgtag ttgctcccat
     4081 aatgccccag cgcactacgc cgcaaatcgc cgagatcaac agcatatcgc gagcactaca
     4141 acggcggaaa agtttattac tcagcgcaaa gataatgact tccgccacca cgcccagcga
     4201 ccacaaatac cccaccgccg aggccgagta gccagctgcc tgccagtaaa tggcgctaaa
     4261 accgtaatag gccgcatgtg ccccctgcaa taaacaaacg caggccagaa agcgccagtt
     4321 ctggcgaacc agcgccaacc acgcagacca accggtgctc tcctgctggc ggcttgcccc
     4381 ttgtggctga atcgtcggac ggatgagaaa gccgagcagc atggatgcca cgcccaacgt
     4441 caacagcacg aggatcaccc gataatcaaa catagtgacc agtttgcccg tcagcgccga
     4501 gccaatgaca aacgccaccg agccccacag tcgcactttg ccgtaatcaa gcgggaactg
     4561 cttttgccac gtattcgcca gtgcatcggt caacggtacc agcggtgaga aaaagaggtt
     4621 aaagccaatc atcaccagca tcagccacgc tacgtgcgcc cccgcccaga aggcgacagc
     4681 aaagagaagt ggcagcagtg ccagcacgcg caaggcggaa atcaggcggg aaggatcgct
     4741 gacgcggggc gcgatgagca aactcccgag aaaacgggca accagacctg cccccaataa
     4801 caggccgatg gtttctggcg ttaaaccaat ccctttaagc cagacgctcc agaaaggtaa
     4861 aaaaatgccg taactaaaaa agtatgtgaa atagccgagc gccaaccagc gcgtggattg
     4921 caaaaccatg agtccctccc gtcaggaggc gttagtctgg ggttaatcga atgttggtgc
     4981 aagttgaaaa ataagaatat tcgagcctgc ttcccgatgt ctgtaacgcc agttcacaag
     5041 cactaaaaaa ctgcgatccg cgacgcatgt tgcaaaaata atgcgtaatt atgccgttac
     5101 gcttgccaaa cgttcctgca caatggcgat gaaatcgcgt aatgccagct tcatctcccc
     5161 tttcttccac gccatcagta aagcaatgga aggcacattt ccggcaatgg gacgaaaaac
     5221 aacctgcccg gtattaaaat tattcatata accgggtatc aaagtgacgc ccagccccat
     5281 gcccaccaga ttcatggtca ccagaatatt cgttgccacc tggacgatat ttggctggct
     5341 gttttcttgc gcaaaccacg ctttaacgat cggcgcaagc gaaccggaat acgccggatc
     5401 ggtactgacg aaattcacgc catccagttg cgcggcggtg atctcttttt catgtgctaa
     5461 agggtgatca accggtaaca ccaccactaa tggttcgtca aaaagctcca gataatcaat
     5521 ctccgggcta taaacaggat ggcgcattaa gccgacgtcg agttcaccac ggcgaatttt
     5581 ttcctcctgt tgcgtggtga ttaaactcac cagctcaatc aaggtgtctg gctgtctgag
     5641 acgaaacatc ggtaatactt ttggcaataa attcacttcc gccgatggca caaagccaat
     5701 ggttaattgt ctgtcttcct gaacaatttt ccgcgcccgt aatttggcat tttccgcttg
     5761 ttcgaggatt gccagcgcgt cctggagaaa acattctccc gccgccgtca acgcgacttt
     5821 gcgcttatcc ctcaccagta acggaacacc gacacagttt tcaagatcgc ggatctggct
     5881 gcttaacgaa ggctgtgagg tatgcagttt ttccgccgca cgggtaaagt tcagtgcctg
     5941 cgccactgcg acgaaatagc gtaaatgccg tagttccatc accttcccct tgttatcgaa
     6001 aaaacgtctc agctggtaga aattaactat ttcacatatt agcaaccaac cagcaacatc
     6061 cttatggcac aaaaatagaa ggtcaataca tcttatcttt caggattaaa aaatatgacc
     6121 acaccctcag atttgaacat ttaccaactg attgacaccc aaaacggtcg ggtcactccg
     6181 cgtatttata ccgacccgga catttaccaa ctggagcttg agcgtatttt cggtcgttgc
     6241 tggctatttc tcgcccacga aagccagatc ccaaaacccg gtgatttctt taacacctac
     6301 atgggagaag atgcagttgt cgtggtgcgc cagaaagacg gcagcatcaa ggcgtttctc
     6361 aaccaatgcc gccaccgggc catgcgtgtg agttatgcag attgtggcaa cagtcgcgcc
     6421 tttacctgcc cgtatcacgg ctggtcttat ggcattaacg gcgagttgat cgatgtaccg
     6481 ctggaacctc gcgcctaccc acaagggttg tgtaaatccc actgggggct aaacgaagtt
     6541 ccttgtgtgg agagttataa aggactgatt tttggcaact gggataccag cgcaccgggc
     6601 ctgcatgatt acctgggtga cattgcctgg tatctggatg gcatgctgga tcgtcgcgaa
     6661 ggcggcaccg aaattgtcgg cggcgtacaa aagtgggtga tcaactgtaa ctggaaattc
     6721 ccggcagagc agttcgccag tgaccagtat catgctctgt tcagccatgc ttctgccgtt
     6781 caggtattag gggcgaaaga tgatggcagc gataagcgcc tcggtgatgg acaaaccgcc
     6841 cgcccggtgt gggaaaccgc caaagatgcg ctgcaatttg gtcaggacgg tcacggtagc
     6901 ggtttcttct ttactgaaaa accggatgct aatgtctggg tcgatggcgc agtttcaagc
     6961 tattaccgcg aaacctatgc cgaagcagaa caacgtttag gtgaagttcg cgccctgcgc
     7021 ctggcgggtc ataacaatat attccccacg ctttcatggc taaacggcac tgccacactc
     7081 cgcgtctggc atccgcgcgg ccctgatcaa gttgaagtgt gggcgttctg tattactgac
     7141 aaagccgcct ccgatgaagt taaagccgct tttgaaaaca gcgccactcg tgcttttggt
     7201 cctgctggtt ttctcgagca ggatgactcg gagaactggt gtgaaatcca gaaattgctt
     7261 aaaggccacc gcgcccgcaa cagcaaactg tgtctggaaa tggggcttgg tcaggaaaag
     7321 cgccgcgacg acggcattcc tggcattact aactatatct tttcagaaac tgccgctcgt
     7381 ggaatgtacc aacgctgggc cgatcttctg agtagcgaaa gctggcagga agtgctcgat
     7441 aaaaccgccg cttaccagca ggaggtgatg aaatgagtgc gcaagtttca ctggagttac
     7501 atcaccgcat tagccagttt ctctttcacg aagccagtct gctggatgac tggaaatttc
     7561 gtgactggtt ggcacagctc gacgaagaga ttcgttacac catgcgcacc acggttaacg
     7621 cgcaaacacg cgaccgtcgc aaaggcgtcc agccgccgac gacctggatt ttcaatgaca
     7681 ccaaagacca gctggagcgg cgaatcgccc gtctggaaac gggcatggcc tgggcagaag
     7741 agccgccgtc acgcactcgt cacttaatca gcaactgcca ggtaagcgaa accgacatcc
     7801 caaacgtatt tgccgtgagg gtgaattatc tactttatcg ggcacaaaaa gagcgcgatg
     7861 aaacattcta tgttggaacg cgtttcgaca aagttcgccg tctggaagat gacaactggc
     7921 gcttgctgga acgggatatc gtcctggatc aagcggtgat cacttcccat aacctgagtg
     7981 tactgttctg atgaatcgaa tttatgcgtg tcccgttgcg gatgtgccgg agggtgaggc
     8041 tctccggata gatacctcgc ccgtcatcgc cttgttcaac gtgggcggcg agttttatgc
     8101 cattaacgat cgttgcagcc atggtaatgc gtcgatgtca gaagggtatc tggaagatga
     8161 cgccacggtg gagtgcccgc tacacgccgc cagtttttgc ctgaaaacgg ggaaagcgtt
     8221 atgcctgccc gccaccgatc cgctcaccac ttatccagta cacgttgaag gtggtgacat
     8281 tttcatcgac ttaccggagg cgcagccatg agcgatctgc ataacgagtc catttttatt
     8341 accggcggcg gatcgggatt agggctggcg ctggtcgagc gatttatcga agaaggcgcg
     8401 caagttgcca cgctggaact gtcggcggca aaagtcgcca gtctgcgtca gcgatttggc
     8461 gaacatattc tggcggtgga aggtaacgtg acctgttatg ccgattatca acgcgcggtc
     8521 gatcagatcc tgactcgttc cggcaagctg gattgtttta tcggcaatgc aggcatctgg
     8581 gatcacaatg cctcactggt taatactccc gcagagacgc tcgaaaccgg cttccacgag
     8641 ctgtttaacg tcaacgtact cggttacctg ctgggcgcaa aagcctgcgc tccggcgtta
     8701 atcgccagtg aaggcagcat gattttcaca ctgtcaaatg ccgcctggta tcctggcggc
     8761 ggtggcccgc tgtacaccac cagtaaacat gccgcaaccg gacttattcg ccaactggct
     8821 tatgaactgg caccgaaagt gcgggtgaat ggcgtcggcc cgtgtggtat ggccagcgac
     8881 ctgcgcggcc cacaggcgct cgggcaaagt gaaacctcga taatgcagtc tctgacgccg
     8941 gagaaaattg ccgccatttt accgctgcaa tttttcccgc aaccggcgga ttttacgggg
     9001 ccgtatgtga tgttggcatc gcggcgcaat aatcgcgcat taagcggtgt gatgatcaac
     9061 gctgatgcgg gtttagcgat tcgcggcatt cgccacgtag cggctgggct ggatctttaa
     9121 ggaagcacga tgaaagaaaa aacgatcatt attgtcggtg gcgggcaagc ggcggcaatg
     9181 gctgcggcct cgctacgcca gcaagggttc accggtgagc tgcatctgtt ttccgatgag
     9241 cgacatcttc cttatgaacg acctccactc tcgaaatcca tgttgctgga agattccccg
     9301 cagttacagc aggtgttacc cgctaactgg tggcaggaaa acaatgttca tctgcattcc
     9361 ggtgtaacca tcaaaacgct gggtcgcgac acacgagagt tagtgttaac caacggcgaa
     9421 agctggcact gggatcagct ttttatagca accggcgcgg cagctcgacc gctgccgttg
     9481 cttgatgcac tgggagaacg ctgctttacc ctacaccatg ccggtgatgc cgccagactg
     9541 cgagaagttc tgcagcccga acggtcagtc gtgattgtcg gtgccggaac tattggtctg
     9601 gaactggctg ccagcgccac gcagcgcagc gcagcgcagc gcagatgtaa ggtgacagtg
     9661 attgaactgg cggcaaccgt catgggccgt aatgcaccac cgcccgtgca acgctatctt
     9721 ttacagcgcc atcagcaggc tggtgtgcgc attctgctca ataatgccat tgaacatgtg
     9781 gtcgatggtg aaaaagtaga actgacgctg caaagtgggg aaacgcttca ggctgatgtg
     9841 gtgatttacg gtattggtat cagcgccaac gagcaactgg ctcgcgaggc caaccttgat
     9901 actgccaatg gcattgtcat tgatgaggct tgccgcacct gcgatcccgc gatctttgcc
     9961 ggtggcgatg tggcaatcac tcgtcttgat aatggtgcac tacaccgctg cgaaagctgg
    10021 gaaaacgcca ataaccaggc gcaaattgcc gctgccgcaa tgttagggct accgctaccg
    10081 ctactgccgc cgccgtggtt ctggagcgat cagtacagtg ataacttaca gtttattggc
    10141 gatatgcgtg gcgatgactg gctttgtcgt ggcaacccgg aaactcagaa ggcgatttgg
    10201 tttaatctgc aaaacggcgt gcttatcggt gcggtcacgc tgaatcaggg ccgtgagatt
    10261 cgcccaattc gcaaatggat ccagagcggc aaaacgtttg atgcgaaact gctgatagat
    10321 gagaacatcg cgcttaaatc actgtaatca ggataattag cgaatatctc aatgcctggg
    10381 gcgtggcgag gtgcaagagt gtgtattacg tttaaatcac attatcttgc aaagggattg
    10441 gttatgaaca cactacgtta ttttgatttt ggagctgccc gccccgtttt gttattaatt
    10501 gcccgtatcg ccgtggtctt aattttcatt atttttggtt ttcccaaaat gatgggcttt
    10561 gacggtacgg tccaatatat ggcctcgttg ggcgcgccaa tgccgatgct ggcagcgatt
    10621 attgcggtag ttatggaagt gcccgccgcg atattaatcg tgcttggctt tttcacccgt
    10681 ccgctggcgg tgctgtttat tttctacacg ctgggtacgg cggtgattgg tcaccattac
    10741 tgggatatga ccggcgatgc ggttgggcca aatatgatta atttctggaa gaatgtcagt
    10801 atcgctggcg cgttcttgct attggcaatt accggaccgg gggcaatttc tctcgatcgg
    10861 cgttaggaaa gatgccggat gcggcgtgaa cgccttatcc ggcattttat aaattacagc
    10921 cactctaccc gcaacgacat ctctgaggtt gttgattccc ctggtgctag cgcaatgaga
    10981 tcaccccctt ccgggcgatg atgctcatcc ggcgcatggc tcatcggttc cagacagaag
    11041 aaatcaaacg catatccttt atcaaacgca gggtcggaaa caaagatgaa ataacacggt
    11101 gcaggtggcg tcgtttccat gatgatggca taaccttcct gcggctgctc gatgcgggcc
    11161 tgaccattcc atccggcaaa accattgttc acccactgac gcggcaacgg cgcgagctgg
    11221 ttaaaatcca gttcctgcgg tagctgctcg caaaactcac ccgccagcca ctgctcccgt
    11281 tccagccagt aaccgctcgc ctgcgcctga atccgcgttt gtggtgacaa cggaaaataa
    11341 ggatgccagc cggtaccaaa tggcagcgtc tctgcccctt gattggtgac agagagcgtc
    11401 accgtcagcg tatccgccgt taaatgaaac gcctgactta cccgatagtg atagacaccg
    11461 ctgcgatgtt catacaccag acacaaacta tcatcgctgt gcgagacaca ttgccactcg
    11521 cccagccagc catcgccgtg tagatagtgc gcatcccact caacattcgg ttgcagttga
    11581 tactcacgcc cctgccagac aaaacggttg ccgctcaccc ggtttgcaaa tggcaccagc
    11641 ggaaaacatg aggcatcggt tgccacaccg ctttttttac caggacgtaa gagcggcgtc
    11701 gtatcgcgcc agaagccttc gattacgccg ccctggtcag aaacgtccag ctttagcgac
    11761 ccgtgggata aggtatagat ggtcatccgc taactcctta atccgggaag ttaatcacaa
    11821 ctttcccgca tttgccgctc gccatcagca cataggcatc acctgcctgt tctagcgaga
    11881 agcgatgggt aatggcgtta cgcggccaca gcttccagtc agtcaaatcg tgggcgcatt
    11941 tttccatatg gaacagactg gtcacccagg agccgataat ccgccgttga tggtgcatca
    12001 gatcagcgct gacctcgaat tccacttttc cggtttcacc aatgtaaacc acccgccccc
    12061 agtcagcagt ggattgcagt gccagcaagc gacctgcggc attaccggaa caatcgagcg
    12121 caacatccgc gccaccgtgg gtaagttcag caattatctg cggcagacct tcggtggttg
    12181 ctaaatagcc gtgatccatc ccccctaact gttttgccat cgccagacgt tccggcagca
    12241 tatcaacgcc gatgatccgt tttgcaccgc gacctttcgc cagcatcatc gccatcatgc
    12301 cgaccggccc cagaccgacc accagcacgt tatcgctgcc ggaaacttcg ccgcgcaaaa
    12361 tcccttcata ggccgtgcca acgccgcaac tgataaacgc accatcttcg tagctcagcg
    12421 catccggcag gaggatcaga tctttttctt ccgccagcag atattcggca tgaccgccgt
    12481 cacgctgcca gccgtaagcc gcttttcctt tgccagtaca agagatagga aagccgcggc
    12541 ggcagttcgg gcaaaaacca cagccagaaa tgtgatacac cagcacgcgg tcgccctctt
    12601 taaaatggcg gcagccttgc cccatcgcca caatctgccc gcacggttca tgaccgttga
    12661 taaatccctg gtataacggt ttatcgggtg ccgccgccgt ggcacggtgt tgatgataga
    12721 tatagtggac atcgcttccg caaatcccgg aggatttcat tttgatcagt acctggttaa
    12781 tccccggcgt cggcaccgca acttcccgca gatcgacggt tgaatttcct agtaaataag
    12841 cagccagcat cgttttcata aatccctcat taacaatacg attaattttc atccctgcag
    12901 cacccgcgcc aggggcgtta aggtttagcg tttcgcttta ctgcttcgct gggtcagcaa
    12961 gatattcgcc agcaccgcca ccacgatgat gacgccgcgt accacctgct ggaaaaagga
    13021 gttaataccg agcagcacca gaccgttacc gattagcgta atcaccagca caccaagcaa
    13081 tgtaccgaac agggaaccgc gaccgccgga aagtgccgta ccgccgacca cgaccgcggc
    13141 gatgacgtca aactccagac cgtttgcggc acctgcgtta ccggaaccga ggcgtgccgc
    13201 caacaaaatg ccggtcaccg ccgctaataa tcccgaaagg gtaaagataa gaatgcgcac
    13261 tcgacgaacg ttaataccgc acaactgcgc cgccgtggca ttaccgccaa cggcaaaaac
    13321 cgagcgcccg aaggcggttt ttcggctaat gaacacaaac agcgcaaaca acaccatcat
    13381 gatcagcgcg gatactggca caccgagaaa ttgtccgccc agccagtcca gcacctcgtt
    13441 ttcgtcaatc ggcactggca gtgcgttggt cataaacagc cccattccgc gcagagcgct
    13501 ccacagcccc agtgtggcaa cgaaacttgg tacgttaaac acgccgcgca gcaccccggc
    13561 gagcgttccc atcagcgcgc ctaacagcaa caccagcaga caagcaatcg ccaacggaac
    13621 ttcaaattgc agcaaaaatg ccaggcacac cgagacaaaa gccaccatcg gcccaacgct
    13681 gacatcaatt tcaccggaga taataatcag cgtcatcgcc caggcggcaa tcccaatggt
    13741 ggcggcatcg cgcagcacgt tcatctggtt attcaatgag ataaagccag gcgcgttcag
    13801 ggagaagacc agataaagaa tggcaatcac caccagcaaa ccgatctcat taatatggcg
    13861 actgacaaat tgtttgagcg agacgctctt gccctgcggc aatggtaatg acgaagcata
    13921 catcttcagg ttcctcggta aaattcatca gtgcacagac agaatggcgg acatcagctc
    13981 atccacattg accggcgagt gaaactcctg cgagaacgtg ccgtgctgta ataacaggat
    14041 gcggtcacac accagcggca attcctccac ttcactggag ataaacacca cgctttttcc
    14101 ttcggcagcc agctcacgga caatacggta aatctgctgt ttggcttcga tatcgacgcc
    14161 gcgcgttggc tcgtcgagca acaaaatctg gctggcggca tagacccaac gaccgatcac
    14221 cactttttgc tgattgccac cagaaagcgt gccgatgggt gtttcgctac tggcagcctt
    14281 gaccgtcatc cgctgcatca cctcttcggt caggcggcgg atggtggacc attgcagcac
    14341 accgttggtg ctgatttttt gccgattggt cagcactgta ttttcgtcaa cgcctagcca
    14401 gggaatgatc cccgcttctt tgcggttttc tggcgtatag ccaatgccgc gtttcagcat
    14461 gtcgccgtaa tcggggcgca tgattttctc gccgttgata acaatttcgc cctgttcata
    14521 cgtctccagc ccaacaatcg ccttcagcaa ttcactgcgc cctgccccca gcaggccagc
    14581 aatgccaagc acttcgccac gacgtagcgt aaagctgata tcctccagct tgggcttatg
    14641 gcgtaacgca cggacttcca gtacggcctg atccacaatt tcctgagggg caaccggcgc
    14701 aatatcaacg tgatcacgcc ctagcattag cgacacaata tgatgcgtgg aggtgttttc
    14761 gagcatcaca tcgcccgcca cctgaccatc gcgcataacg gtggcatagg aggcaatgcg
    14821 gcggatttct tccatccggt ggctgacata aatcaccgcc acgcccagtg ccgacatctt
    14881 tttcaccgca ctgatcacca gttcaacttc tgcactggca agcgagctgg tgggttcatc
    14941 aagaatgacc acgcgcggct cgcccttcat cacccgtgca atttccacca gttgcttttg
    15001 cgccgggctt agcgttgaaa caagttgttc aggactaacg tcaacgccca gcgcctgtaa
    15061 gcaacgttgg gcatcctgcg ccatttgcag gtaatcaatc atgccgttgc ggcgaggcca
    15121 ttgaccaagg cagaggtttt ctgccaccgt cagcccttcc accagactta attcctgata
    15181 aaccgcacga acccccagtt cagcggcacg gcgagtcaac atagcttcgt caccttccag
    15241 tcgcgtctcg ccaatccaga tatcaccgct atccgggcgt tcgctgccgg taagcattcg
    15301 aatgagagtc gatttgcccg cgccgttttt acctaacagc gcacgaactt cgcctttatt
    15361 gagcgtgaag ttaacgttat ccagcgcaac gacgccgggg taacgcttat ttcctgccac
    15421 cacttttgct accgggactg cctctgttgc cgtgaacata gggacctctg cgaatcagcg
    15481 attagggcag accatcaacg tgcgttgcca gccactgttt accatcttcc gttttggtat
    15541 agagatcgat tggcacctga atcacttttt caccgtcggc ttgtttattg ataaccttca
    15601 atgtttgcgc gaaaacagca ttgcccattt tcttaccgga aatatccact accgctttca
    15661 gcacctgatt gttttccagc tcctgagcaa tttcggttgt catatccgaa ccgaaaacag
    15721 caatttttcc ggcctgattt tgattacgta ccgcttttac cgcgccgagt gtcgcaccgc
    15781 ccgactcccc cataatggcg ttgagatccg gcgtggagat aatcagtttt tcaccaacgg
    15841 aaatcgcttt atctaaaaca gtcccttcct ggttagcgac aatttgcgcg ccgggaacgc
    15901 gggattttaa tacttcttca aatcctttac gtcgctgcac acaaacttca aaggcttcgc
    15961 aattgatgac ggcaattttc ggctggtcaa ttttattggc aataaaataa tcggcggcag
    16021 cgttacccag tttttaccaa attccagtgg atcgccgacc agatacgccg agacatattt
    16081 atcgacaccc ttttgattaa tacaggtgtt gtagcaaatc accggaatac ccgcttcact
    16141 ggcgcgacga acggtacggc tactgccatt ttcagacact gccgataaaa taatggcatc
    16201 gacattacgc gccacgaggg tatcaacaaa ggtactttct ttcgaaatat ccccctgggc
    16261 gttagtttca attaactgta cctgtactga agaatctttt gccgcatcct gaacgccctg
    16321 acgcactcca gcgtaatatc cctgggtatc aaggtatatt gcaccaatgg tcatttcttt
    16381 atccgcagcc ctggcaaata gtgcgctacc taatagcgta gccattaata atagattacg
    16441 ggttgttctc atttttttag gcatagtgcc tcctgtaggg tttttattaa caacggctta
    16501 ttctaattat tttgtgatga gcggcagcgc gtgccgctca aatattacag ttaatagggt
    16561 ttagtgaacg tagttaaaaa taaaaggcat cacagtggtg aataatgccg ctttcggcca
    16621 ggccggatta atcgccgtca attcagcgcg agcagactca tattgcgcaa cctccccgag
    16681 tcccgcggac gccagcatcg ccaccatcag gcatttttct ttatgctgct gttgtaaatc
    16741 gccatacagc gataacaggt caggttgtga aacagcaaag aaatccgcct caatactggt
    16801 tttcgccatc tcttgcgccc actgtttcat ttcactaaac agttgctgtg cggtttgctg
    16861 ttcacccagc agtcgcagcg ccattccttg ccagaagaga taatcaaccg gctgatcgtt
    16921 gtaataactg tggatgttaa tggtgcgatc gccggtcgcc gccagacgta aacaacgcat
    16981 cgcttcagtt tcatcgccct gcgcgttggc gcatatcgcc tgccagaacc agatgtcgtt
    17041 atcagtttgc cccggtaaac ggccttcgct taaattctcc ggataataca gcgcggcatg
    17101 aagcagttcg ctggcctgct gcggctctct ggcatcaaga tgctgccagg cgcgtaataa
    17161 ctggttgagg ataaactgac tggtgacctt cccttccccg ccttcccacg ggtggaattt
    17221 acgcgtggcg agaatgtccg ccgctttgtc tgcctgaccc gtgagatgcc acaaattgag
    17281 cagttctgcg gtcatgtcgt cgcgtttcag cgcaatttcc agattatttt ccagacgcgc
    17341 cagtcgtttc tccggtgtgg ctccacttaa cttatccagc aaatcccgtt cgaaaagcag
    17401 acgtgcatcc tgcggcgcaa gctgataagc attatcaaga taacgcgcgg ccagctcata
    17461 atcgtgtttc ttattccacg catggatcgc taacccgcgc cagccgtcgg caaactccgg
    17521 cgacatctct acgcaacgtt gccataaggc aatggctttg ttgtagctac gtttgttgta
    17581 gtagaagcag gccagtaaat ggcgagcaaa ccaacactct tcaatactct ccagcgccgc
    17641 cacttcttcc agcgtattcg ggaaacggac aaactgcggg aagacatcaa tggcttttgc
    17701 gaccagttcg ccacgttcgg ctttcggcag caagctggct tgcaggtaaa gcggcagcgt
    17761 gcgctggcag tccagtgcgt tcagcatctc tgccgccagg gtgggcattc cccagttaat
    17821 cagttgcccg gcggtcatca gagcgttaac gtcgcgtccc tgacacagac cgcgccactg
    17881 cgcgagggct gattcgctac gaccatcgaa ccagttcagc caccacagag tggcgttcag
    17941 cggataatca cgcagcagtt tctcgcgctg caaacgcgcg ttgtcctgac gaccacttaa
    18001 caccagcagc agattatgca agcaaagcac ttcctgattg gttgggcagg cacgaagact
    18061 ttgttggcaa aaatccagac ccgcgtcgaa gttatcatta cgcgccgcca gtcgtgccag
    18121 accataatag ccgcctgctt tactattgcc gctccagacc gcacgccaga aatcctcttc
    18181 ggcttgttga tattgtccct gacgttcgta agcactggcg cgaatcaaac ttgcttgtcc
    18241 acactgcggt tttttgttca gcgcatgtgc acgtttcagc gcctgactgg catacgccac
    18301 cgcttgcggg aaatctgcgc ggttatattc cagcatcgcc agcgccaggt tacagcgata
    18361 atccagcggg tccagcgcca cgccgcgcag gtagtaatcg aacggtgaac ggctggcgtg
    18421 atgatattgc tccagatgct gaccgataaa ccaggcttca tctgtactgg taatgtcttg
    18481 tgctgccagt ggcgctttgg cgacgtccgg caacggcaac tcctgcggct gatgttcctg
    18541 ataactcagt acaatattgc cgtcggcatc ggagagttca atggtcagtc tgtcaggatt
    18601 gataccgtgc aacacgccct ggatggcggt cgctggtgtc agggccacgg catcatcgag
    18661 caacgcgttg catttgccga tttcgcggat cgccagacgg tatccgttca acggagagat
    18721 ggcatacagc ccccactcaa tcccccgctc actacgctgg agttttatca ccgcatcgcg
    18781 ggaggcattt tgcaccatgc ccagagaatg ataaggcagg aaatactgct cgaaacgctt
    18841 ctcttcgtaa gcatcaagcc aggtaaaatc aggctggtta tcggcaaaaa taccggtcat
    18901 cagttcgata tacgggccgt tattgtcggt cagactctta tcccacgcct ggccaaattc
    18961 actgtgtccc caactccact gttttttacc tggcgcaata tggtggttgg caacgtgcag
    19021 caaaccgcca tcttcatcgt gacaccacgc gccgacaaaa tcgtactgcg atttttcggc
    19081 catatatgag gttggaacag gcacattttt atagcgagaa atgtccactc cggcggagta
    19141 gtccactttg tagtaagtgc cggtggcgat ggggaaagcg gagacggccc gtttgccgtg
    19201 atcaaacacc gcagtcacat ccggtgggaa aacgctctga tgcccttcac cccctttcac
    19261 tgccgggttg acccaccaca agaaatgacg cggcgtggcg ttgccgttat agacgcggct
    19321 ggcgatttcc agtgccgccc ggtcagggcg cagggtgaaa cctgtcatca cctgtaaacc
    19381 atgcatcggc tccgtttcgc cgacccacac cgtctgtgca ccgtcttcat gggcttcgag
    19441 ggtgaaatca acgggcataa aggtggtcgg gcgatggtgt tgcggccagt taaactcaat
    19501 cccgccggag atccacggtc ccagcatccc caccagcgca ggtttaatga cttcattgtg
    19561 atagacaaaa tcgcgctgtt tcactttatc ccatgcgcga tgcacccgac cgcccagttc
    19621 cggcaggatc atcactttga tgtagtcgtt ttccagccac accgcctgct aggatttcag
    19681 ggttttctgc tcgctcagcg tatcggtcac gccgtaggga taaaccgcgc cggacgatcc
    19741 ctgataaacg cgattttcca ggaacatggg atgtatatcc tgcggcccgg tttcataggt
    19801 cgggatctca acgcgctctt gccacacttt tactggagtc atggtgccct caatattaac
    19861 aagacatact gaattaaaag atttgtggca gtgtattgaa caatctggca atgttttcgc
    19921 ggaataatca cgcaattaac taaacaaggt ttagtgaaga tgagagcctg cattaacaat
    19981 caacagattc gccaccataa caaatgcgtg attctggaac tgctgtaccg gcaaaagcgc
    20041 gccaataaat caacgctggc ccggctggcg caaatttcga ttccggcagt cagtaatatt
    20101 ttgcaggaac tggaaagcga aaaacgggtg gtgaatattg acgatgaaag ccagacgcgc
    20161 gggcatagta gcggtacatg gctgattgcg ccggaaggtg actggacgct gtgcctgaac
    20221 gtgacgccca ccagtattga gtgtcaggtt gctaatgctt gtttaagtcc gaaaggcgaa
    20281 tttgagtatt tacagattga tgtaccgacg ccgcaggcgc tgctgtccga aatcgaaaaa
    20341 tgctggcatc gccaccgtaa attgtggccg gaccgcacca tcaatctggc gctggcaatc
    20401 cacgggcagg ttgatccggt gaccggcgta tcgcaaacca tgccgcaagc gccgtggaca
    20461 acgccagttg aggtgaagta tctgctggaa gagaagctgg gcattcgggt gctggtcgat
    20521 aatgactgcg tgatgctggc gctggcggag aaatggcaaa ataattcgca ggaacgggat
    20581 ttctgcgtga tcaacgttga ttacggcatt ggctcgtcgt tcgtgattaa cgagcaaatt
    20641 tatcgcggca gtttgtatgg tagcggacag attggtcaca ccatcgttaa tccggatgga
    20701 gtcgtctgcg actgtggacg ttacggcagc ctggaaaccg tcgcctcgtt aagtgcatta
    20761 aaaaaacagg cgcgggtatg gctaaaatca caaccggtta atactcagct tgagcctgaa
    20821 aaactgacaa ccgcgcagtt aatcgctgcc tggcagagtg gagaaccgtg gatcaccagc
    20881 tgggttgatc gctctgccaa tgccattggt ttgagtctgt ataacttcct caacatcctc
    20941 aatattaatc agatttggtt gtacggtcgc agttgtgcct ttggtgagaa ctggcttaat
    21001 actattattc gccagacagg atttaacccg ttcgaccgcg acgaaggacc gagcgtgaaa
    21061 gcgacgcaaa ttggcttcgg gcaattaagc cgcgcacaac aggtgctggg aattggctat
    21121 ttgtatgttg aggcgcagtt acgacagatt tgatggcgcg ataacgtaga aaggcttccc
    21181 gaaggaagcc ttgatgatca taaacgaaaa attgcctgat gcgctacgct tatcaggcct
    21241 acacggagat tgcaatatat tgaatttgca agattttgta ggcctgagaa ggtgttcatg
    21301 ccgcatccaa catgaacaac gagcacattg tcagcaaatc accgtttcgc ttatgcgtaa
    21361 accgggtaac gtgcgcagat gtcgagaact ttacctttga tgcgctcgat aacggcttca
    21421 tcattgatgc tgtccagcac gtcacacatc cagccagcca gttctttcgc ttcggcttct
    21481 ttaaagccgc gacgggtaat cgccggagta cctacacgaa taccggaggt cacaaacggg
    21541 ctcttcggat cgttcggtac gctgtttttg ttgacggtga tgttagcacg gcccagagcg
    21601 gcgtctgctt ctttaccggt caggttttta tcaaccagat caaccaggaa caggtggtta
    21661 tcagtgccgc cggaaaccac tttgtagccg cgctcgagga acacttctac catcgcttta
    21721 gcgtttttcg cgacctgctg ctggtaagtt ttgaactcag gctccatcgc ttctttcaga
    21781 gcaaccgctt taccggcgat tacgtgcatc aacggaccgc cctgaccacc agggaaaacg
    21841 gcagagttca gttttttgta cagctcttcg ctaccacctt tcgccaagat caggccgccg
    21901 cgcggacccg ccagggtttt gtgagtggtg gtagtaacaa cgtgagcatg aggaaccggg
    21961 ttcgggtaga cgccagcagc aaccaggccc gcaacgtgcg ccatatcaac gaacagataa
    22021 gcaccgatgc tgtcagcgat ttcacgcatt ttcgcccagt caaccacgcc ggaatatgca
    22081 gagaaaccac cgataatcat tttcggcttg tgttctttgg cttgtttttc cagatcggcg
    22141 taatcgatat gaccggtagc atcgataccg taaggaacga tgttgtacag tttaccggag
    22201 aagttaaccg gagaaccgtg agtcaggtga ccgccatgcg ccaggttcat acccagaacg
    22261 gtatcacctg gttccagcag cgcggtgtag accgcaaagt tagcctggga gccggagtgc
    22321 ggctggacgt tagcgtagtc agcgccgaac agttctttcg cacgatcgat cgccagttgt
    22381 tcaacgatat caacatactc gcaaccgccg tagtagcgtt tgcccggata accttcagca
    22441 tatttgttgg tcagctgaga accctgcgcc tgcattacgc gcgggctggt gtagttttcg
    22501 gaggcgatca gttcgatgtg ctcttcctga cgtacttttt cctgctccat agcctgccac
    22561 agttcggcat cataatcggc aatgttcatt tcacgcttta acatccgcat ctcctgactc
    22621 agctaacaat aaaatttttg gcctttatag gcggtcctgt tggacaacgg cgaacagtat
    22681 aaccgaatca ttgtgcgata acaggtcttg acaaaggaat ttacgcaaac gattaccttc
    22741 aggctacgca aggctttgga gaataaagag cttgcaaccg gaaacggatt tcttttcagg
    22801 tttgtgatgc aaatttttca cttcatcaca ttctttctga aaaacaccaa agaaccattt
    22861 acattgcagg gctatttttt ataagatgca tttgagatac atcaattaag atgcaaaaaa
    22921 ggaagaccat atgcttgacg ctcaaaccat cgctacagta aaagccacca tccctttact
    22981 ggtggaaacg gggccaaagt taaccgccca tttctacgac cgtatgttta ctcataaccc
    23041 agaactcaaa gaaattttta acatgagtaa ccagcgtaat ggcgatcaac gtgaagccct
    23101 gtttaacgct attgccgcct acgccagtaa tattgaaaac ctgcctgcgc tgctgccagc
    23161 ggtagaaaaa atcgcgcaga agcacaccag cttccagatc aaaccggaac agtacaacat
    23221 cgtcggtgaa cacctgttgg caacgctgga cgaaatgttc agcccggggc aggaagtgct
    23281 ggacgcgtgg ggtaaagcct atggtgtact ggctaatgta tttatcaatc gcgaggcgga
    23341 aatctataac gaaaacgcca gcaaagccgg tggttgggaa ggtactcgcg atttccgcat
    23401 tgtggctaaa acaccgcgca gcgcgcttat caccagcttc gaactggagc cggtcgacgg
    23461 tggcgcagtg gcagaatacc gtccggggca atatctcggc gtctggctga agccggaagg
    23521 tttcccgcat caggaaattc gtcagtactc tttgactcgc aaaccggatg gcaaaggcta
    23581 tcgtatcgcg gtgaaacgcg aagagggtgg gcaggtatcc aactggttgc acaatcacgc
    23641 caatgttggc gatgtcgtga aactggtcgc tccggcaggt gatttcttta tggctgtcgc
    23701 agatgacaca ccagtgacgt taatctctac cggtgttggt caaacgccaa tgctggcaat
    23761 gctcgacacg ctggcaaaag caggccacac agcacaagtg aactggttcc atgcggcaga
    23821 aaatggcgaa gttcacgcct ttgctgatga agttaaggaa ctggggcagt cactgccgcg
    23881 ctttaccgcg cacacctggt atcgtcagcc gagcgaagcc gatcgcgcta aaggtcagtt
    23941 tgatagcgaa ggtctgatgg atttgagcaa actggaaggt gcgttcagcg atccgacaat
    24001 gcagttctat ctctgcggcc cggttggctt catgcagttt accgcgaaac agttagtgga
    24061 tctgggcgtg aagcaggaaa acattcatta cgaatgcttt ggcccgcata aggtgctgta
    24121 atttgatgtt gccggatgga aacatccggc aacccttgac gcggtttaaa ttgccgcgtc
    24181 gtcctcttca ccggtacgga tgcgaatgac ccgagccacg tcaaagacga agattttacc
    24241 gtcaccgatt ttgccggttt gcgccgtgcg aataatggta tcgacacagg tatcgacaat
    24301 gtcgtccggt acgacaatct caattttcac tttcggcaga aaatccacca tatactccgc
    24361 gccgcggtac agctcggtat ggcctttctg gcgaccaaag cctttcactt cggtcaccgt
    24421 catgccggta ataccgactt cggccagtgc ttcgcggaca tcgtccagct tgaagggttt
    24481 tataatcgca tcaatctttt tcatgctatt ccttgaaaag gttgcctgtc ttttgatctg
    24541 ctaaacgtaa cacataacgc caattcattc cttgaaatcg tttgcatcca gctcgtgtcg
    24601 ggaaagcagt ttataaaatt ctgtccggtt gcgccccgcc attctcgccg cgtgggtgac
    24661 gttgcctttg gtgatttgca gcagcttacg caagtagttg agttcaaaat gattacgtgc
    24721 ctcaacaaag gttggcagcg ccgtattttc accctccagc gcctgctcca ccagcgcatc
    24781 actaatcacc ggagatgagg tcagcgccac gcactgttca atcacgttga ccaactggcg
    24841 cacattaccc ggccagctcg cggtcatcag gcgtttcatc gcatcggtag agaacgcgcg
    24901 gacaaacggt ttatgtcgct ctgccgcctg gcgcaacagg tgatttgcca gtagcggaat
    24961 gtcttctgtg cgctccgcca gtgccggaat tttcaggctg acaacgttga ggcggtaata
    25021 gagatcttcg cggaattccc cgcgcgccat cgcttttgac agatcacggt gagtggcaga
    25081 aataatccgc acattgatat caatatcgcg gttactgccc agcgggcgca ctttacgctc
    25141 ctgcaacacg cgcagcagtt tgacctgtaa cggcgcaggc atatcgccaa tttcatcgag
    25201 aaatagcgtt ccgccttccg ccgcctggaa taaaccttcg cgattgctga cagcgccagt
    25261 aaacgcgcca cgcgcatgac caaacagctc cgactccagc aattgctcgg gcaatgcgcc
    25321 acagttaata gcaataaatg gtttgctgtt gcgcgggctg gcgttgtgga tagcctgggc
    25381 gaaaatctct ttcccggtgc cgctctgacc gttaatcaaa acgctgacgt ctgattgcgc
    25441 caccagccgc gcctgttcca gcaaacgcag catcagcggg ctgcgggtga caattgcctc
    25501 gcgccagcgt tcatcggtgg ctggcgcgga ttgctccagc gcatcgtcaa ttgcctgata
    25561 tagcgcatct ttatcgacag gcttggtgag gaaactaaaa acgccctgct gtgttgcagc
    25621 aacggcatcg gggatagaac catgcgcagt aagaataatt actggcattc ccggctgcac
    25681 tttctggatt tcagcaaata gctgcatacc gtccatttca tccatccgca ggtcgctgat
    25741 aactaaatct actttttcgc gattcagtac ccgtaatcct tcagcgccac tttccgccgt
    25801 gaccacactg tagccttcgc tggtcaggcg caggccaagc agtttcagca atcccggatc
    25861 gtcatcgacc aataataaat gcgcaggttt atggctcatc aggagtgacc tcatgggtgg
    25921 atggcgcggg cttttcactc tcgtgcggcg tatccggcga gaaatttcca gccggtttgc
    25981 gggtcgagag ctggcgttca atatcggtca ggttttccag cttgcgggtg gtgagttcca
    26041 gttgctgctg tagaacgtga tgttgctggc gcaatgtatc cagctcgctg tcgctggact
    26101 gctggagttt actgtaacgt tggcgctctt ccgccagttg cagttgcagc gcctgaccgt
    26161 cgcgccagag ttgatacagt gggcgaacct gtgccgggat ctcggtactt aacgcttcaa
    26221 tacgcgcgac cagctggcgg cgctcatacg gcgtaatttt ggcgtcggcg agcaaaatcc
    26281 cttgtttaaa ggtattttgc cagctgccgt cgtcatattg gcgagcttgc tgacgcgact
    26341 gcgcaggcat taaacgatca gcacaatcca tcgcccgcag ccagtaaagc ggattggttt
    26401 cggttgattt accttgcagc gcccagatgt cgctacattc agtagaaaga tagtcagcca
    26461 gttgataaac cggaattttc tcttctgctg gcgtatcaat ggctggctta ttgtgattct
    26521 gcacgcaacc cagcaatgcc agacatggca gccctgccag ccacagccgt cggggcaata
    26581 atcgttgaaa aatgtgtcgc atattcacca gacttaaagc ctatcccagt gggcgtaatt
    26641 gttgcagaca gtctggacat ggacagcgcg gagaaaccgg ggcgtacata tcgtacgtga
    26701 agatttcgag cactacccgg ggccgaactg acaaataaaa tagcctgata ggataggctg
    26761 ttagcattat ttcgtgtttt tcgacgacgg taattcaatg cggaaacaaa cgtcttgccc
    26821 gctctcgtcg accagataca gttccccttg catacggcga atacaatccc tggcaatgct
    26881 taatcccaga ccgctgccct tcaccgcccc ttttcgctgg tggcttccct gaaaaaaggg
    26941 ttcgaagatc atggcgcgtt cctcttgcgg aatgggcgtg cctgtattga tgacatcaat
    27001 ataaacccgc gcaccatgta aactgctgcg aaggcaaatg ttaccggatt cagccccgta
    27061 gtgcaccgca ttggagtaaa gattatccag tacgctcatc agcagcattg gctccgccag
    27121 gcaagctgtt gctttgagat cgacgtcggt atgcatcatt ttagcccgtg cgggcaggct
    27181 atgagcagaa accactgtct ccaccagcgg tgctaattca acattctcca gttccaccgc
    27241 actgtccgcc tgtttacggt tgtaatcaag cagttgttcg atcagttttt gcaaattgca
    27301 gctgctgcta tcaagaatgc tcaccacctc tttttgctct ggcgtaagcg gcccgacaac
    27361 ctgatcagcc agtaattcag tgccctcgcg catactcgcc agtggcgttt ttaattcatg
    27421 agataaatgt cttaaaaatt gatggcgttg ggattccagc catgacaggc gctcacttaa
    27481 ccaaagaata cgttgcccaa ccgagcgtaa ctcgctcggt ccacagaacg aaacgctatt
    27541 gcccagagaa cgcccttccc ccagacggtt gatcatgcgc tcgatatttt tcaccggccc
    27601 gataatcatc cgcgtgaaaa gtagcaccat caccagactc accagaaata gcaccagcga
    27661 ttgccaacca aaatattgcc cacgttcggc gatttcacgc tgaagttgct gcccacgaga
    27721 gaacaccact gtgcgcgtgg cctgcaccat ttcggtattg gcactggcaa aggcttcaag
    27781 acgcgcggcg gcggcagcat cgggaccact gttgttacac tgaagttggg ccagattgtt
    27841 caaatcctga cgcaatgcct ggtaaagttt atcgtctggc agtacgcctg cgtgggcatc
    27901 gagcatttcg ctgtaacgct tgcgctggct ttgataaacc ttcgcaagcg tagggtcgtc
    27961 cagcacgcaa tactgacggt agctacgctc catctccagc gccgcgttgg tcatcgcttc
    28021 actacgccgg gcatcaataa gcgtagtgcg gttaaccagc gccgcctgat cgctaagcgc
    28081 attcaggctt tgccaggctt gccatgccag caccaacagg ggtagtagaa ttagcaaaaa
    28141 tgccagcatt accagttgtc gtaatgagcg gggaaaaacg ggccagcgtt tcaaggtgtt
    28201 actctcgtca gacgcgaata gcctgatgct aaccgagggg aagttcagat acaacaaagc
    28261 cgggaattac ccggctttgt tatggaataa ggcggtgcct aactcgacgt ttcgcccgat
    28321 ggttgatata gctacgctga tatcagaagt tggacggcag gcaccttgtt gtgcgtcatt
    28381 cgaattttat gtagcacgtc ccgaaggggc tgacataagt cggtgaatga gccactgctt
    28441 actattatgc agtaactgtg ccaataaaga aaatagtttg gtaacgtact gattatacgt
    28501 tgctttgagg gatttatggc ttcttcgcta tttgaatata aatcagccac tgtgtcgcta
    28561 aaaagagaca acttaagata aacttattag attgcaataa aatcaatcat ttaagtgtcg
    28621 ccagaaagcg acacggtaaa ccacccattg tcgtgattta cagacacaaa aaagctcccg
    28681 gagttgggag cttatgatag tggttggtgc ttaatgccgc atccgggctg caaaaccaat
    28741 gaactgatga cttaccccaa ctgcttacgc gcattgcgga aaatgcgcat ccatgggcca
    28801 tcctcgcccc agttttccgg atgccaggag ttgctgacag tacggaaaac acgttccggg
    28861 tgcggcatca taatggtgac tcgaccactt tcagtcgtaa ccgccgtaat accgttcggt
    28921 gaaccgttcg ggttagccgg gtaggtttca gtgactttgc cgaagttatc gacatagcgc
    28981 agtgccacca gccctttgct ttccagtgcc gccagatgcg ccgcatcacg cacttccacg
    29041 cgcccttcac catgagagac agcaatcggc atttgcgagc ccaccatccc ctgcaacagc
    29101 agagacgggc tttgggttac ttcaaccagg ctgaaacgcg cttcaaagcg atcggaggtg
    29161 ttacgcacaa aacgtggcca caactcactc cccgggatca gttcacgcag gttagacatc
    29221 atctggcaac cgttacatac ccccagggcc agcgtttgcg gacgatggaa gaaggttgca
    29281 aactcatcgc gtacacggtc attgaacagg atagacttcg cccaaccttc accggcaccc
    29341 agcacatcac cgtaggagaa acctccgcac gcgaccaggg cgtgaaaatc ctccagtccc
    29401 gtacgtccag tcagcaggtc gctcatatgc acgtcgatag catcaaagcc cccacgttgg
    29461 aaagctgccg ccatttcaac atgcgagttc acgccctgct cacgcagtac ggcaactttc
    29521 ggacgtgcgc cagtggcaat atacggtgct gccacatctt cgttgatatc gaacgacagt
    29581 tttacattca ggcccggatc ggcgtcgtta gatttcgcct gatgctcctg atcggcacac
    29641 tccgggttgt cacgcaggcg ctgcatctgc caggtagttt ctgcccacca gacacgcaac
    29701 gtagtgcggc tttcgctgaa tacagtctgc ccgttggcgg taatcacaaa acggtcaccg
    29761 gaaaccgcct gcccaacata atggacgcaa tccgcaagcc catgctgtgc cagtacggac
    29821 tcgaccgctt cacggtcagc ggcacgaacc tgaatcaccg cacccagttc ttcgttaaac
    29881 aacgccgcca ggcgatcgtc acccagagag gcgatatccg cgtcaatgcc acaatgacct
    29941 gcaaaggcca tttccgccag cgttaccagc aggcctccat cagagcggtc gtgatacgcc
    30001 agcagcttac gctgtgcaac cagcgcctga atcgcgtcat agaagccttt cagttggaca
    30061 acatcgcgta catctgccgg tttgtcgcca agctgacgat aaacctgcgc cagcgccgtt
    30121 gcgcccagcg cgttattgcc tttgcccaaa tcaatcagca gcagtgcgtt atcttcggta
    30181 caaagctgcg gcgtgatggt gtgacgtaca tcttccacgc gggcaaatgc agaaatcacc
    30241 agcgacagcg gcgacgtcat ttcgcgctct tcgttacctt cctgccagcg ggttttcatc
    30301 gacatagagt ctttacccac cgggatcgtc aggcccagcg ccggacaaag ttcttcaccc
    30361 acggctttaa cggcttcata caggcccgca tcttcaccag ggtggcctgc cgccgccatc
    30421 cagttggcgg aaagtttaat gcgtttgata tcgccaatct gtgttgcggc aatattggtt
    30481 aacgcttcac cgaccgccag acgggcagag gcggcgaaat ccagcagcgc aaccggcgca
    30541 cgctcgccaa tcgccatcgc ttcaccgtag tagctgtcga ggctggcggt agtgaccgcg
    30601 cagttagcga ccggcacctg ccacggcccc accatctgat cgcgcgccac catgccggtt
    30661 acgctgcggt caccgatggt caccaggaaa gttttctccg ccacggtcgg cagatgcagt
    30721 acgcgtttca ccgcgtctgc aatggtgatc ccttcacgga ccagcgcatc gcccttcgct
    30781 ttcagcgttt gtacatcgcg ggtcatcttc ggcgttttac caagcaggac gtccagcggc
    30841 agatcgatcg gctgattatc aaaatgacga tcgtgcagag aaagatgcag ttcttcggtc
    30901 gcttcaccaa tcaccgcgta gggtgcgcgc tcacgcttac acagttcgtc aaacagcggt
    30961 aattgatcgg cagcaaccgc cagcacgtag cgttcctggg attcgttaca ccagatttcc
    31021 agcgggctca tgcccggttc gtcgcttaga atatcgcgca gttcaaattt accgccgcgt
    31081 ccgccgtcgc tcaccagctc cggcatcgcg ttagaaagac cgcccgcacc gacgtcgtgg
    31141 ataaacagaa ttgggttagc atcaccaagc tgccagcaac ggtcgatcac ttcctggcaa
    31201 cgacgctcca tctccgggtt gtcgcgctgt acggaagcaa agtcgaggtc ggcatcagac
    31261 tgaccagacg ccatggaaga cgccgcgccg ccgccaagac cgatgttcat tgccgggccg
    31321 ccgagaacga ccagcttcgc accgacgttg atctcgcctt tttgtacgtg atcggcgcga
    31381 atgttgccga tcccgcccgc cagcatgatc ggtttgtgat aaccgcgcag ctcttcgcca
    31441 ttgtggctgt tcactttttc ttcataagta cggaagtagc cgttcagtgc cggacgacca
    31501 aattcgttgt taaacgccgc accgcccagc gggccttcgg tcatgatgtc cagcgcggta
    31561 acaatgcgct
//
LOCUS       DAJTSI010000032        31185 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000032 DAJTSI010000000
VERSION     DAJTSI010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31185
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.032"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            389..2047
                     /locus_tag="OO327_001328"
     CDS             389..2047
                     /locus_tag="OO327_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR6017935.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2044..3000)
                     /locus_tag="OO327_001329"
     CDS             complement(2044..3000)
                     /locus_tag="OO327_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR6017936.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3151..4266
                     /gene="macA"
                     /locus_tag="OO327_001330"
     CDS             3151..4266
                     /gene="macA"
                     /locus_tag="OO327_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR6017937.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4263..6209
                     /gene="macB"
                     /locus_tag="OO327_001331"
     CDS             4263..6209
                     /gene="macB"
                     /locus_tag="OO327_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR6017938.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="OO327_001332"
     CDS             complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="OO327_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR6017939.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6829..7149
                     /gene="clpS"
                     /locus_tag="OO327_001333"
     CDS             6829..7149
                     /gene="clpS"
                     /locus_tag="OO327_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR6017940.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7180..9456
                     /gene="clpA"
                     /locus_tag="OO327_001334"
     CDS             7180..9456
                     /gene="clpA"
                     /locus_tag="OO327_001334"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR6017941.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9859..9946)
                     /locus_tag="OO327_001335"
     tRNA            complement(9859..9946)
                     /locus_tag="OO327_001335"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9910..9912),aa:Ser,seq:gga)
     gene            complement(10200..10418)
                     /gene="infA"
                     /locus_tag="OO327_001336"
     CDS             complement(10200..10418)
                     /gene="infA"
                     /locus_tag="OO327_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR6017942.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10703..11407)
                     /gene="aat"
                     /locus_tag="OO327_001337"
     CDS             complement(10703..11407)
                     /gene="aat"
                     /locus_tag="OO327_001337"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR6017943.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="OO327_001338"
     CDS             complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="OO327_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR6017944.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="OO327_001339"
     CDS             complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="OO327_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR6017945.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="OO327_001340"
     CDS             complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="OO327_001340"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR6017946.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16569..17063
                     /gene="lrp"
                     /locus_tag="OO327_001341"
     CDS             16569..17063
                     /gene="lrp"
                     /locus_tag="OO327_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR6017947.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17198..21226
                     /gene="ftsK"
                     /locus_tag="OO327_001342"
     CDS             17198..21226
                     /gene="ftsK"
                     /locus_tag="OO327_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR6017948.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21381..21992
                     /gene="lolA"
                     /locus_tag="OO327_001343"
     CDS             21381..21992
                     /gene="lolA"
                     /locus_tag="OO327_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR6017949.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22003..23346
                     /gene="rarA"
                     /locus_tag="OO327_001344"
                     /gene_synonym="mgsA"
     CDS             22003..23346
                     /gene="rarA"
                     /locus_tag="OO327_001344"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR6017950.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23437..24729
                     /gene="serS"
                     /locus_tag="OO327_001345"
     CDS             23437..24729
                     /gene="serS"
                     /locus_tag="OO327_001345"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR6017951.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24969..27412
                     /gene="dmsA"
                     /locus_tag="OO327_001346"
                     /pseudo
     CDS             24969..27412
                     /gene="dmsA"
                     /locus_tag="OO327_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27423..28040
                     /gene="dmsB"
                     /locus_tag="OO327_001347"
     CDS             27423..28040
                     /gene="dmsB"
                     /locus_tag="OO327_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR6017952.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28042..28833
                     /locus_tag="OO327_001348"
     CDS             28042..28833
                     /locus_tag="OO327_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR6017953.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28869..29495)
                     /locus_tag="OO327_001349"
     CDS             complement(28869..29495)
                     /locus_tag="OO327_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6017954.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29810..30958
                     /locus_tag="OO327_001350"
     CDS             29810..30958
                     /locus_tag="OO327_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6017955.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         7689 a         7645 c         8373 g         7478 t
ORIGIN      
        1 tggtcgtttt atagggtcgt cgtaaaaaat gacgacggaa ataatgcgcg gctattttaa
       61 aaacgaaggc gagtcattca ccagataaat aaatccagta aatttgattt agggcaacag
      121 cgggttgccc catatagtca tttgtctgat tgacagtgta gtgcaagcaa aagatttaat
      181 cctttaggcg taataaaaaa taatttatca tgctaattat ttgattttgt tgtttttgcg
      241 ggcttatcag caagagggag tataacgcga ttattcgctc atttttcaga caattgccat
      301 gcttaaatgt gatgtcatca cgtattagca aggcctttcc cgttatactg cgagcgtaaa
      361 ggataagtca catatttctg gaggggatat gattcttgag cgcgttgaaa ttgtgggttt
      421 tcgcggtatc aaccgtttgt cgttgatgct cgaacaaaac aacgtcctga ttggggagaa
      481 cgcgtggggt aaatccagct tgctggacgc cttaaccctg ctgctatcgc cagaatcaga
      541 tctctaccat tttgagcgcg acgatttctg gttcccgccg ggagatatca acgggcgaga
      601 acatcatctg catattattt tgaccttccg cgaatcgctg ccaggccgac atcgggttcg
      661 ccgttatcgg ccgctggaag cgtgctggac gccatgcacc gatggctatc accgtatttt
      721 ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg atgacactgc gcagttttct
      781 cgataaagac ggacatccga ttgatgttga ggatattaac gatcaggcac gccatctggt
      841 gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta ttcgtaacgg
      901 cacggtgcca aatgtcccca atgtggaagt caccgcgcgc cagctcgatt tcctcgcccg
      961 tgagttgtcc tcacatccgc aaaatctctc tgatggacag attcgtcagg gactttccgc
     1021 aatggtgcag ttgcttgagc attatttctc tgagcagggg gctggacagg cgcgatatcg
     1081 tttaatgcgg cggcgggcca gcaatgagca acgaagctgg cgctatctgg acatcatcaa
     1141 ccggatgatt gaccgacctg gagggcgctc gtatcgggtt attttgctcg gcctgtttgc
     1201 tactttattg caggcaaaag gcacattgcg actggataaa gacgcgcgtc cgttattgct
     1261 aattgaagat ccagaaaccc gcctgcatcc gattatgctt tccgttgcct ggcatctgtt
     1321 gaatcttttg cctttacagc gtattgctac caccaactct ggcgagctgc tttcgttaac
     1381 tccagtggaa catgtttgcc gcctggtgcg tgaatcttct cgtgttgccg cctggcgtct
     1441 ggggccaagt ggcttgagca ccgaagacag ccgccgcatc tcttttcaca ttcgttttaa
     1501 tcgtccgtca tcgctgtttg ctcgctgctg gttgctggtg gaaggggaaa cggaaacctg
     1561 ggttatcaat gaactggcgc gtcagtgcgg acatcatttt gatgccgaag ggatcaaggt
     1621 cattgagttt gcccagtccg ggctaaagcc actggttaaa tttgcccgcc gaatggggat
     1681 tgaatggcat gtactggtcg atggcgatga agcagggaag aaatatgccg ctacggtacg
     1741 cagcctgttg aataacgatc gggaagccga acgagaacat ttaacggcgt taccggcgct
     1801 ggatatggaa cattttatgt atcgccaggg attttccgat gtgttccacc gcgtggcgca
     1861 aatcccggaa aatgtaccga tgaatctacg caaaattatc tcgaaagcga tccatcgctc
     1921 ttccaaaccc gatcttgcca ttgaagtggc aatggaggca ggacgtcgtg gtgtggactc
     1981 cgtaccaacg ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc gcggtcgcgc
     2041 ggattaaccg cgaaacatcg tggccatttg tggctgaata gcgtcgagca tctcatagcg
     2101 ccgacggtat tcagcccgtt ttttactggc gatttcggca atctcttttc gtgctatctg
     2161 tgctggaagg cggtaatggc gttcagcatc acatacgccg ccaaccgact cccagaacgc
     2221 gttgtaatca gcatggatct tgccttcttt atcgcgataa cgcaggctgc ggtaaatatg
     2281 cgtttcattg ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa acagacaggc
     2341 cgcttccatc acgaggcgtt tgggaaatag cccgtggcag gctttcgtcg cattctggat
     2401 ttcctgatgt ggaatttccc attttgcgcc ttgcagtccg ccaataaaca tcgttctttt
     2461 cccctgatat tcacacaggg taaacgtgat ctctgccaga ggaatacctt cgctgttgcg
     2521 gaacaggatt gtgctgtcac cttctttatc cattgagatc atcatggtca gctcaagcgt
     2581 gaactgctca ccgtttttgc cttccagttt agccagttgc agcccggggg tattcaaata
     2641 taagctgaat tcttccgccg acatacaccc gcggagtaac gcataatggt aacgtaacgc
     2701 ctccagcaat tgcttacggc taagattcgc ggcaaggtaa gggcgatgca gacgcacagg
     2761 cagtcgcggc tggcgcgtta acaacacatt gagattaggc cagtgggaaa gttcgttcat
     2821 ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct ggcgaaaact
     2881 acagcgatgc cagaatttac ccggccgaca ctgtccacgt gccagactaa gaaaaagtga
     2941 caggctgctg agagattcag atggcgtaaa ggtccgttca gttagctgcg acatattcat
     3001 gaaatcaatg gttatacatg acgtcgattt caccattgcg tatcttaacc aaacatcaat
     3061 agtgtgatta ctaacgtaaa ttttagggtt ttgttgatat ttcgttgaag ttaatgaccc
     3121 ggattggcat atggagtatt cagaatattt atgaaaaagc ggaaaaccgt gaagaagcgt
     3181 tacgttattg cgctggtgat agtcatcgcc ggactgatta cgttatggag aattcttaac
     3241 gcacccgtgc cgacttatca gacactgatt gtgcgccccg gtgatttaca gcaaagcgtg
     3301 ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg tgggcgcgca ggtcagcggt
     3361 cagttgaaaa cgctgtcggt ggcgattggc gataaagtaa aaaaagacca gcttttaggg
     3421 gtcattgatc ctgaacaggc tgaaaaccag atcaaggagg tcgaagcaac gctgatggag
     3481 ctacgcgcgc agcggcagca ggcggaagcg gagctgaaac tggcgcgggt gacgtattcc
     3541 cgtcagcaac gtctggcaca aacgcaagcg gtttcactgc aggatctcga taccgccgcg
     3601 acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat caagcgcaat
     3661 caggcctctc tcgatacggc taaaaccaat ctcgattaca cccgtatcgt tgccccgatg
     3721 gctggggaag tgacgcaaat caccactctg caaggccaga cggtgattgc cgcacagcaa
     3781 gcaccgaaca ttctgacgct ggcagatatg agcactatgc tggtaaaagc gcaggtttct
     3841 gaagcggatg taatccacct gaagccgggg caaaaagcct ggtttacggt gcttggcgat
     3901 ccactgacgc gctacgaggg gcaaatcaag gatgtgctac cgacgccgga aaaggtaaac
     3961 gacgctattt tctattacgc ccgttttgaa gtacccaacc ccaatggttt gctgcggctg
     4021 gatatgaccg cgcaagtgca tattcagctc accgatgtga aaaatgtgtt gacgatccct
     4081 ctgtcggcgt taggcgatcc ggttggcgat aatcgttata aagtcaaatt gttgcgtaat
     4141 ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta acgataccga tgttgagatt
     4201 gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg aggccaaacc aggagctgca
     4261 caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc ggtgatgagc
     4321 aggttgaggt gctgaagggc atcagcctca atatttatgc gggtgagatg gtcgcgattg
     4381 ttggcgcttc gggttccggt aaatcgaccc tgatgaatat tctcggctgc ctggataagg
     4441 ccaccagcgg cacctatcgc gtagccggtc aggatattgc cacgctggac gccgatgcgc
     4501 tggcgcaact gcgccgcgag catttcggct ttattttcca gcgttaccat ttgcttccgc
     4561 atttaaccgt cgagcagaac gttgaagtac ccgccgtcta tgccggtctt gagcggaaac
     4621 agcgactgct tcgtgcccag gagttgctgc aacggctggg gctggaagac cgtacagagt
     4681 attatccggc acagctttcg ggtggtcagc aacagcgcgt cagcatcgcg cgggcattga
     4741 tgaacggcgg tcaggtaatt cttgccgatg aaccgaccgg cgcactggac agccattctg
     4801 gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg cgggcatacg gtgattatcg
     4861 tcacccacga tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt cgcgacggcg
     4921 aaattgtgcg caatcctccc gccattgaaa aagtgaatgt tgctggcggg acggagcctg
     4981 ttgtcaatac agtgtctggc tggcggcagt ttgtcagcgg ttttaacgag gcgctgacga
     5041 tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt actgaccatg ctggggatta
     5101 ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg tgacgccgcc aaacaaatgg
     5161 tgctggcgga tattcgttct attggtacga atactattga tgtctatccc gggaaagatt
     5221 ttggcgatga cgatccgcaa tatcagcagg cgctgaagta cgacgactta atcgccatcc
     5281 aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg cgcctgcgtt
     5341 ataacaatgt tgatgttgct gccagtgcca atggcgtgag cggcgattat tttaatgtct
     5401 atggcatgac cttcagtgaa ggaaacacct ttaatcagga gcagctgaac ggtcgtgcgc
     5461 aggtcgtggt tctcgacagt aatactcgcc gccagctttt cccccataaa gcagatgtgg
     5521 ttggcgaggt gattctggtc ggcaatatgc ccgccagagt cattggtgtg gcggaagaaa
     5581 aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac agcacgatgt
     5641 ccgggcgagt tatgggccag tcgtggctta actccattac tgtcagggtg aaagaaggat
     5701 ttgacagcgc cgaggcggaa cagcaactca cgcgtttact ttcactgcgc cacggtaaga
     5761 aggatttctt tacctggaac atggacggcg tcttgaaaac tgttgaaaag accacacgta
     5821 ctttacaact gtttctgacg ctggtggcgg tgatttcgct ggtggtgggc ggtattggtg
     5881 taatgaatat tatgctggtg tcagtgaccg agcggacgcg ggaaattggc attcgcatgg
     5941 ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct gatcgaagcc gtactggttt
     6001 gcctggtcgg tggcgcgttg ggaataacac tgtcactgtt aattgctttc accttgcagc
     6061 ttttcttacc cggctgggag attggttttt caccgttggc gctgctgctg gcgtttctct
     6121 gctcgacggc caccgggatt ttatttggct ggttacccgc acgaaatgcg gcacgactgg
     6181 atccagtaga tgctctggca cgggagtaat ttttgagata aaaatgccag ccgatcgggc
     6241 tggcattttg cctttaggat gtacacaatg agacagaaga gctatgcgac tgccgcttct
     6301 acttcgacgg gcacaataac actggcgtga ttgccttttg gcccctggtg gacatcaaac
     6361 tgaacggatt gtccagcttt tagcgttctg taaccatcca tctgaatggt ggaataatga
     6421 gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac caaacccttt ggcattgttg
     6481 aaccacttaa cagtaccctt ttccatgctt cgacatcctt cgcaaatctt atacaagtaa
     6541 gatggaataa accggggtca gagagggggc tgttcaaaac ctcgccaact ctagaaatac
     6601 aatttagaga attagggcga gccgtcaagc atttgacagg ggccaggggg caggtatgaa
     6661 tcaaaaattt gaagcagtta acgctattga caggaatgtg acagatgtcg ctgatgccaa
     6721 cgatagatga tagttatcta tcatgtggag tagattggtc aggcaaataa gctcttgtca
     6781 gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat gggtaaaacg
     6841 aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc gctaaaaccg
     6901 ccatctatgt ataaagtgat attagtcaat gatgattaca ctccgatgga gtttgttatt
     6961 gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt gatgctcgct
     7021 gttcactacc aggggaaggc catttgcgga gtctttaccg ccgaggttgc agaaaccaaa
     7081 gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg tacgctagaa
     7141 aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca agaactggaa
     7201 ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt tatgaccgtc
     7261 gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct ggaagcgtgt
     7321 tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca aaccacaccc
     7381 gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgctgagttt tcagcgtgta
     7441 ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggttac cggtgcaaac
     7501 gttctggtcg ctatcttcag cgaacaggag tcgcaggcgg catatctgtt gcgtaaacat
     7561 gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg gcacgcgtaa agacgagccg
     7621 acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg tggggaggaa
     7681 cgtatggaga atttcacgac gaacctgaac cagcttgcgc gtgtgggcgg aatcgaccca
     7741 ctaattggtc gtgagaaaga gctggagcgt gctattcagg ttctctgccg tcgccgtaaa
     7801 aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc agaaggtctt
     7861 gcctggcgaa ttgttcaggg tgatgtgccg gaagtgatgg ctgactgtac gatttactct
     7921 ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg gcgactttga aaaacgtttt
     7981 aaagcgttgc tcaagcagct ggagcaggac actaacagca tcctgtttat tgatgagatc
     8041 cacaccatta tcggtgcggg tgcagcgtct ggtggtcagg tcgatgcggc taacctaatc
     8101 aaaccgttgc tctccagcgg taaaattcgc gtaattggtt cgacaaccta tcaggagttc
     8161 agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat tgatattact
     8221 gaaccgtcga tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa gtatgaagcg
     8281 caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg cggtagagct ggcggtgaaa
     8341 tacattaacg atcgtcatct gccggataaa gccattgatg ttatcgacga agcaggcgct
     8401 cgcgcacgtc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc ggatattgag
     8461 tccgtggtgg cccgtattgc acgcattcca gagaagagtg tttctcagag tgaccgcgat
     8521 accctgaaaa aactcagcga tcgcctgaaa atgctggtct tcggccagga taaagccatt
     8581 gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga acataaaccg
     8641 gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga aaacagaggt gacggtacag
     8701 ctttcgaaag cgttgggcat tgagcttctg cgctttgata tgtccgagta tatggaacgc
     8761 cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga tcagggcggt
     8821 ttgctgaccg atgcggtaat caagcatcca catgcggtgc tgctgctgga cgaaatcgag
     8881 aaagcgcatc cggacgtgtt caatattctg ttgcaggtga tggacaacgg tacgctgacc
     8941 gataacaacg gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac cactaacgcc
     9001 ggggtacggg aaactgagcg caaatccatt ggtcttatcc accaggataa cagcaccgat
     9061 gcgatggaag agatcaagaa gatctttaca ccggaattcc gtaaccgtct cgacaacatt
     9121 atctggttcg atcatctgtc aactgacgtg atccatcagg tggtggataa attcatcgtc
     9181 gagttgcagg ttcagctgga tcagaaaggt gtttctctgg aagtgagcca ggaagcgcgt
     9241 aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg cacgtccgat ggcgcgtgtc
     9301 atccaggaca acctgaaaaa accgctcgcc aacgaactgc tgtttggttc gctggtggac
     9361 ggcggtcagg tgacggttgc gctggataaa gagaaaaatg agctgactta cggattccag
     9421 agtgcacaaa agcacaaggc ggaagcagcg cattaatctg attgtcaggt aggttggtga
     9481 agtccgtaat ctcgtcaggg gttacggact ttttatttat ggggtggaac ggtgaaaacc
     9541 ctatttttgg aggtgaaggt aagttgttga taattagtgc tgctggaagg taaggataaa
     9601 aaagggtgct gcaggagaat gggatggttt tgctttatta acaacgggct aaacgtgtag
     9661 tatttgagtt cactgccgta caggcagctt agaaatcgac ggggtgcggt aaaacctttg
     9721 cgaacgcgtt cactgccgta caggcagctt agaaattcac aggtaacata ctccacccgc
     9781 ccaccatgtt cactgccgta cagacagata aaatgcgaaa aaaaagctcg cactttcgta
     9841 cgagctcttc tttaaatatg gcggtgaggg ggggattcga acccccgata cgttgccgta
     9901 tacacacttt ccaggcgtgc tccttcagcc actcggacac ctcaccaaat tgttttgcta
     9961 ccaaacctca tgggtggcaa cggggcgcta ctatagggag ttggagtaaa acggtcaaga
    10021 agaattttaa tgataattat tgtttgctca tactgtaaac aacttgtgca gtatatctac
    10081 atcgagacaa gttatggact tatacttcca aagtacttca tacatatcac aaaataaaaa
    10141 ggccggttaa accgaccttt tactcgttct ttctcttcgc ccatcaggcg gtaaaacaat
    10201 cagcgactac ggaagacaat gcggcctttg ctcaggtcgt acggggtcag ttcaacagtc
    10261 actttgtcgc ccgtcaggat gcggatgtag tttttgcgca ttttaccgga gatgtgtgca
    10321 gtaaccacgt gaccgttttc taactctacg cggaacatgg tattaggcaa cgtttcaaga
    10381 acggtacctt gcatttcaat attgtcttct ttggccatct aatcctctgg ggtatcacta
    10441 ccgtaatttg aaccggcaag ataatgccga agttctgtaa ataagtaaag atttgcgcgc
    10501 taaatcgcaa caaacaggtt cggcacatta ctccgaaaac acacggctaa gccgcaccaa
    10561 aagcgcaacg tataagggag cggtgagata aacgatgggc gttacctgac gcgaaaaatt
    10621 ccttatcggc agcggggtaa tgagcgtaac caactctgcg accgcaatta taacactctg
    10681 gggagaaatg tgccgaaaac attcattctt gtggtgaaaa caagcaccgt ggtacccaga
    10741 aattattcgg caatcgtccg aggcgcattt gattgagata attaaggtaa tcccggcggg
    10801 gaatttcgca ggcaccaagc gatgctgtgt gatcgttaag gacctggcag tcgataagct
    10861 taccgccatg accgataaat tcctcacaga ataccagaag cgccgtttta gacgcatttt
    10921 ccatccggct gaacatggac tcgccacaaa atagcgttcc ctgggccacg ccgtacatac
    10981 cgccgacaag ctcatcttca cgccagactt caatggagtg ggcatgaccg agttcgtgaa
    11041 ggcgatggta ggcttcgacc acgccacgcg tgatccaggt tccttcttcg cgatcgctgg
    11101 cacagccttc aatgacctga ccaaaagcgt aattcatcgt gacacgatag ggcgagcgtt
    11161 tatgaaatcg cttcatacta cggctgatat gcagtgattc tggccatagc accgcgcggg
    11221 gatcgggcga ccaccagagg atggggtcgc ctggagaaaa ccacggaaaa ataccacgct
    11281 ggtaagccat taacaggcgc gcagggctaa gatcgccccc aagtgccagc aggccgttag
    11341 gctcacgtaa tgcgccttcc ggggaaggga aggctattga atggcgagaa agctgaacca
    11401 agcgcatgac cgcaaaactc cacgcaagtc ggatcgttca ataatagctt acaaaccctg
    11461 cttgaactgg taataacgcc cctgtctggc aagcagttct gcgtgagtac cttgctcaat
    11521 aatttgcccg ttgtccatca ctattatttg ttggaaacga gagagtccgc gaagtcgatg
    11581 ggtgaccatt aacaccgttt tctcacgcat catttttgca agcaattcaa ggatctggct
    11641 ttcggttgtg gcatctaagc cttcggtagg ttcatccagc aacaccagtg gcgcatcatg
    11701 taacagcgta cgggcgatag ccagacggcg cagttcacca ccggagagct ggcgtccgcc
    11761 ttcacctaac caactgttga gacctgcatc ctcgagcagc ttctccagac caacgcgacg
    11821 caagatctcc gacagagcct catcactact gccaggcgag gcgagtaaca gattatcacg
    11881 cagtgtggcg ctaaacagat gcactcgctg aggaacaacg ctgatggtct gtcgtagagc
    11941 cgcttcattc aggctggcta tggggctatc gttaagcaaa atctcgccct gttgcgggtc
    12001 ccatgcgcgg gtcagcagtt gtaacagtgt tgatttgccg catccggttc gcccgagaat
    12061 cgctatatgt tccccggcgt ttacctgaag agaaatccct ttaagtgcct gttgggattg
    12121 ctccggataa gtgaactgaa catcccgtaa tgtcagcgaa acgcgatcgg caacacgagt
    12181 ttgggtatca gggaaggtga cctccggttt ttgatccgtt aagtcagaga tacgtacggc
    12241 agaggcaatg acttgcccca gatgctgaaa tgcacccgtt actggtgcca gtgcttcaaa
    12301 cgcggctaac gcacaaaaga caaacagggc aattaatgcg ccgggttgag cattgccgcc
    12361 aacgccgcca gacgccatcc acagcatcag gatcaccgct aacgcgccaa tgagcagcat
    12421 tatcgcttgc gacaatgcgg tcagttcaga ttgacggcgt tgcgcttcca gccattgaat
    12481 ttctgtattc tctagttgcg tgcgataacg atcgctggca ccaaaaatgg tcagctcagc
    12541 ttgcccttgc agccaggccg tcagttgttg gcgatactgt ccgcgaagat gagtcagatt
    12601 ttgcccggtg ctttttcccg cacgataaaa cagcggtggc atcaggaaaa gcgtcagtaa
    12661 cataacgctg cccagcgtaa aggcgagggt gaaatcaagg aaacttaacc cgattgtcac
    12721 caccataatc accacaaaag cgcccaccag cggcgagata acgcgcaggt aaagatgatc
    12781 gagcgtatca acatccgcca ccacgcggtt gagcaattcg ccctgacgat agcgcgccag
    12841 tccggcaggg gagaggggca acaatttgct gaaggtgtaa atgcgcagat gctgcaacac
    12901 gcggaaagtc gcgtcgtgac ttaccagacg ttcaaaataa cgcccggcag tacgggtgat
    12961 tgcggcacca cgcacgcccg cagcgggtag catatagttg aagctgtaca gtccggcaac
    13021 ccccgcaacc gctgaggccg agaggaacca gccggaaagt gtcaacagac cgatactggc
    13081 gagcagcgtc acaattgcca gcacaatacc aagacttaac atccatttat gacgtttata
    13141 cagtgccaga tagggtagca aagcgcgcat ttaaatctcc tcctgacgat gggccagtaa
    13201 tgtggcgaat gggccaccag ccacacttaa ttccgcgtaa cgtccttgct caataatccg
    13261 gccatcctgc ataacccaaa tgacatccca gtcagcaaga tcttctaact ggtgggtgac
    13321 cattaacgtt gtctggcgca gagaggcggc attcagcgcc tccattacgc gctgttcact
    13381 gtgagcatca aggctggcag cgggttcatc caacagtaat agcgaacagg gatttagtaa
    13441 cgcacgggcc accgccacgc gctgcgcctg ccccacggaa aggcgggcag cctggtcgcc
    13501 aacaggtgta tcaacgcctt gtggcaggag cggtagaaac tcgctgaccc aggcgttatc
    13561 cagcgctgct tgtaattctt gttcgctggc atcaggtcgc gccagtagta cgttatcccg
    13621 caattttgct gccggtaatt gtgggttttg cccaacccag gagagatgtt tacgccatga
    13681 ttctggtgat aaatcacgta attctatccc gttgattcgt aatgatccct gatatgagag
    13741 aaaaccagaa agcgcgttca gcagtgagct tttacctgaa ccgctgcggc caaccaacac
    13801 tgcacgttgg cctgctggca aagtaaagtt cagcggtccg gccagcgttt taccttccgg
    13861 cgacgtgata aacagatcct cggcctcaat ggtcagcgga tcggtcaatg ctaattccgc
    13921 ctcaccgcgc tgcggatggg cgagcggggt ttccataaac gttttcagac tgtcagctgc
    13981 accaacagcc tgggctttag catgataaaa cgtaccgaga tcgcgtaatg gctggaaaaa
    14041 ctctggcgca aggatcaggg ccagaaaacc tgcagccagc gtcaccccag tatcgtagtg
    14101 accaaaatcc agctcgccga gataggaaaa accaaagtag accgccacca gagcaattga
    14161 cagcgaggta aaaaattcga gaatgccgga ggataaaaac gccagtcgta gcacttccat
    14221 tgtccgttgg cggaaatctt ccgaagcaga acgaatactt tcaatttcag cttcaccacg
    14281 accaaaaata cgcaatgttt ccatgccgcg caggcgatcg aggaaatgcc cacttaagcg
    14341 agcaagagcg agaaagttac gccggttagc atcggcagcc cccattccaa ccagcgccat
    14401 aaacaacgga attagcggtg ctgtgcccag cagaatgagc gccgcagccc agttagaggg
    14461 gaagatcgcc accacaatca gcaacggcac cgacactgcc agcgccattt gcggcagata
    14521 gcgtgcatag taatcatgca tatcgtcaat ttgctcgagt accagcgtcg cccagctccc
    14581 cgcaggttta ccctgaatcc acgctggccc tgcttgttgc agacggtcga gaacctgacg
    14641 gcggatggca aagcggatat gctgcccggc gtgataaccc acccgttcgc gtaaccagac
    14701 cacccatgcg cgcaatacaa aggtcagaac cagtaacgta aagggaagca gcagggcttc
    14761 acggggaata ttctccataa tcatatgttg cagaattcgc gccatgaacc aggcctgggc
    14821 aatgatcaat atgccgctta caaagcccag cagacgagaa atattcagcc aacgttggga
    14881 gatgacgctt tgctgtttta accagcgggt taactctttt tgacgagatt tattcattgc
    14941 acgcttagca ggtgagttat cagaattatt tgcagagcaa tgttacaacg gggaaaaaat
    15001 aaaggcgacc catagtcgca aggtgtcgcc ttctttactt ttgttactga tttgtaaaat
    15061 tattttgcgt cagctaaacc atcgaggtag cgttccgcat caagtgctgc catgcagcca
    15121 gtaccggccg aagtaatggc ctggcgataa atgtgatcca tcacgtcgcc tgcggcaaag
    15181 acgccaggaa tgctggtctg ggtggcatta ccatgaatac ccgactgtac tttgatgtag
    15241 ccgttttcca gttccagctg cccttcgaaa atcgcagtat tcgggctgtg accgatagca
    15301 acaaacagac cggcaacgtc gagtgactcg atgttatcgc tgttttgcgt atcgcgcaga
    15361 cgaacgccag tgacacccat ttgatcgccg gtcacttctt ccagcgtacg gttggtgtgc
    15421 agaatgatgt tgccgttctc cactttatcc atcagacgct taataaggat tttttccgcg
    15481 cggaaaccgt cacggcggtg aatcagatgc acttccgaag cgatgttaga cagatacagc
    15541 gcctcttcaa ccgcggtatt gccgccgccg atgaccgcaa ctttctggtt gcgatagaag
    15601 aaaccgtcgc aggttgcaca agcagaaacc ccacggcctt taaaggcttc ttcagagggc
    15661 aggccgagat agcgtgcaga agctccggtg gcaataatca gcgcgtcgca agtgtattcg
    15721 ccgttatcgc cattcagacg gaacggacgg ttttgcagat ccaccttgtt gatatgatca
    15781 aaaatgatct cagtttcaaa cttggtggca tgttcgtgca tgcgcgccat taataacgga
    15841 ccggtcagat cgtttggatc accaggccag ttttccactt ccgtggtggt ggtcagttgg
    15901 ccgccttttt ccatgccggt aatcagcaca ggttgcaggt tggcgcgcgc cgcgtagaca
    15961 gcagcggtgt atcccgccgg gcctgaaccc aggataagca gtttactgtg tttggtcgtg
    16021 cccatgagat ccccatagtt gttggcagac aatgggcagg attgtaggga atttacagac
    16081 gtaaaaaaag agtatgacga ttttgttaac aatttgtgca atcggcagca tcgataagca
    16141 ggtcaaattc tcccgtcatt atcacctctg ctacttaaat ttcccgcttt ataagccgat
    16201 taaatgatga ataaacgccc ctgttaatga atatctggca tgttgtacta aaaatcgatg
    16261 ttttgctttg acaatcccct ggtgttttgc gaaaacattc gaggaagaaa aaaacagtat
    16321 tcttatatgc gcataaccat gtatgtaaat accatgttta ccgtgctagt gaaatctacg
    16381 tatggcgtgg acagacgcca ttcgtgatgt cgatagctgc cacaaggcaa cggtcttctc
    16441 accgtagacc caggcattgc gcgccgtgaa tcttcatgat ttcggtctat cgtgacgggt
    16501 agcgactctg aacagtgatg tttcagggtc agacaggagt agggaaggaa tacagagaga
    16561 caataataat ggtagatagc aagaagcgcc ctggcaaaga tctcgaccgt atcgatcgta
    16621 acattcttaa tgagttgcaa aaggatgggc gtatttctaa cgtcgagctt tctaaacgtg
    16681 tgggactttc cccaacgccg tgccttgagc gtgtgcgtcg gctggaaaga caagggttta
    16741 ttcagggcta tacggcgctg cttaaccccc attatctgga tgcatcactt ctggtattcg
    16801 ttgagattac tctgaatcgt ggcgcaccgg atgtgtttga acaattcaat accgctgtac
    16861 aaaaacttga agaaattcag gagtgtcatt tagtatctgg tgatttcgac tacctgttga
    16921 aaacacgcgt gccggatatg tcagcttacc gtaagttgct gggggaaacc ctgctgcgtc
    16981 tgcctggcgt caatgacaca cggacatacg ttgttatgga agaagtcaag cagagtaatc
    17041 gtctggttat taagacgcgc taacacggaa caggtgcaaa atcggcgtat tttgattaca
    17101 ctcctgttaa tccatacagc aacagtactg gggtaacctg gtactgttgt ccgttttagc
    17161 atcgggcagg aaaagcctgt aacctggaga gcctttcttg agccaggaat acactgaaga
    17221 caaagaagtc acattgacaa agttaagtag cggccgccgc cttctggagg cattgctgat
    17281 ccttattgtc ctgtttgccg tctggttgat ggctgcctta ctaagcttta acccttcgga
    17341 ccccagctgg tcgcaaacgg cctggcatga acctatccat aatttaggtg ggatgcccgg
    17401 tgcgtggttg gcagatacgc tgttctttat ttttggcgtg atggcttaca ccattcccgt
    17461 cattattgtc ggcggttgtt ggtttgcctg gcgtcatcag tccagcgacg aatacattga
    17521 ttattttgcc gtttcgctac gcatcattgg cgttttggcg ctcatcctta cctcctgtgg
    17581 tctggcggca atcaacgctg acgatatctg gtattttgcc tccggtggcg tcattggcag
    17641 cttactaagc actacgctac aaccactgct acacagtagc gggggaacta ttgcgctgct
    17701 ctgcgtttgg gcagcgggcc tgacgttgtt caccggttgg tcatgggtga ccattgctga
    17761 aaaactcggc ggctggattt taaacattct caccttcgcc agtaatcgta cccgtcgcga
    17821 tgatacctgg gtcgatgaag atgagtatga agacgacgaa gagtatgaag atgaaaatca
    17881 cggcaaacag catgaatcac gccgtgcccg tattcttcgc ggcgcgctag cgcgtcgtaa
    17941 acggttggcg gaaaaattca ttaatccgat ggggcggcaa acagacgctg cgttgttctc
    18001 cggtaagcgg atggatgatg acgaagagat tacctacact gcacgcggtg tggctgccga
    18061 cccggacgac gtcctatttt cgggcaatcg tgcaacgcag ccagaatatg acgaatacga
    18121 tccattatta aacagtgcgc caattaccga acctgtcgct gtagcagctg ctgctaccac
    18181 ggcgacacaa agctgggctg cgccggttga acctgtgact cagacgccgc ctgttgcctc
    18241 tgttgatgtt ccaccttcgc aacctacagt agcctggcag cctgtaccgg gtccacaaac
    18301 gggagagccg gttattgctc ctgcaccgga aggttaccca cagcagtcac aatatgcgca
    18361 gcctgcagtg caatataatg agccgctgca acaaccagta cagccgcagc agccgtatta
    18421 tgcacctgca gctgaacaac ctgcgcaaca gccgtattat gcccctgcgc cagaacaacc
    18481 ggtggcaggt aacgcctggc aagccgaaga gcagcaatcc acttttgctc cacagtctac
    18541 ataccagact gagcaaactt atcagcagcc agccgctcag gagccgttgt accaacagcc
    18601 gcaacccgtt gaacagcagc ctgttgtgga gcctgaaccc gttgtagaag agacaaaacc
    18661 cgcgcgtccg ccgctttact attttgaaga agtggaagag aagcgagccc gtgaacgtga
    18721 acaacttgcg gcctggtatc aaccgattcc agaaccggtt aaagaaccag aaccgatcaa
    18781 atcttcgctg aaagcacctt ctgttgcagc agtacctcca gtagaagccg ctgccgctgt
    18841 ttctccgctg gcatctggcg tgaaaaaagc gacactggcg acgggggctg ccgcaaccgt
    18901 tgccgcgcca gtcttcagtc tggcaaatag cggtggaccg cgtccgcagg tcaaagaggg
    18961 gattggcccg cagttgccac gaccgaaacg tatccgcgtg ccaactcgtc gtgaattggc
    19021 gtcttacggt attaagctgc cctcacagcg tgcggcggaa gaaaaagccc gtgaagccca
    19081 gcgcaatcag tatgattctg gcgatcagta taacgatgat gaaatcgatg cgatgcagca
    19141 ggatgaactg gcacgtcagt tcgcccagac acagcagcaa cgctatggcg aacagtatca
    19201 acatgatgtg cccgtaaacg cagaagatgc agatgctgcg gcagaggctg aactggctcg
    19261 tcagtttgcg caaactcaac aacaacgtta ttccggcgaa caaccggctg gggcgaatcc
    19321 gttctcgctg gatgattttg aattttcgcc aatgaaagcg ttgctggatg atggtccaca
    19381 cgaaccgctg tttacgccaa ttgttgaacc tgtacagcag ccgcaacaac cggttgcacc
    19441 acagcagcag tatcagcagc cacaacagcc ggttgcgcca cagcagcagt atcagcagcc
    19501 acaacagcag gttgcgccgc agccacaata tcagcagccg caacaaccgg ttgcgccaca
    19561 gcagcaatat cagcagccgc aacaaccagt tgcgccgcag ccgcagtatc aacagccgca
    19621 acaaccggtt gcaccgcagc cgcagtatca gcagccacaa cagccagttg cgccacagcc
    19681 gcaggatacc ctgcttcatc cgctgttgat gcgtaatggc gacagccgtc cgttgcacaa
    19741 accgacgacg ccactgcctt ctctggattt gctgacaccg ccgccgagcg aagtggagcc
    19801 ggtagatacc tttgcgcttg aacaaatggc gcgtctggtg gaagcgcgtc tggctgattt
    19861 ccgtattaaa gccgatgtcg ttaattactc tccggggcca gttatcactc gctttgaact
    19921 taacctggct cctggcgtaa aagcggcgcg catttctaac ttgtcacggg accttgcccg
    19981 ttcactttcg acggtggcgg tgcgtgtcgt tgaagttatt cctggcaaac cctatgtagg
    20041 tctggagtta ccaaataaaa aacgacaaac cgtttatctg cgcgaagttt tggataacgc
    20101 caaattccgc gataatccgt cgccattaac cgtggtgctg ggtaaagata tcgccggtga
    20161 gccggtggtt gccgatctgg cgaaaatgcc gcacttgttg gttgcgggga ctaccggttc
    20221 cggtaaatct gtcggtgtga acgcgatgat cctgagcatg ctttataaag cacagccaga
    20281 agatgtgcgt ttcatcatga tcgacccgaa aatgctggag ctttcggttt atgaaggcat
    20341 tccgcacctg ttaacggaag tcgttactga tatgaaagat gccgccaacg cgctgcgctg
    20401 gtgtgttaac gagatggagc gtcggtataa actgatgtct gcgctgggtg tgcgtaatct
    20461 ggcgggttat aacgaaaaaa ttgctgaagc cgatcgcatg atgcgtccga ttccagaccc
    20521 gtactggaag ccgggtgaca gtatggatgc ccagcatccg gtgctgaaaa aagaaccata
    20581 cattgtcgtg ttggttgacg aatttgccga cctgatgatg acggtaggta aaaaagtgga
    20641 agagctgata gctcgtctgg cgcaaaaagc ccgtgccgcg ggtatccacc tcgtactggc
    20701 aactcagcgt ccatcggttg atgttattac tggtctgatt aaagcgaata ttccgacccg
    20761 tatcgccttt accgtatcca gtaagattga ctcacgtacc attcttgatc aggctggcgc
    20821 ggaatcactg ctgggtatgg gggatatgct ctactctggg ccgaactcca cgttgccggt
    20881 acgtgtccat ggtgcttttg ttcgcgatca ggaagttcat gccgtggtgc aggactggaa
    20941 agcgcgtggt cgcccacagt atgttgatgg catcacctcc gacagcgaaa gcgaaggtgg
    21001 tgcgggtggt ttcgatggcg ctgaagaact ggatccgttg ttcgatcagg cggtgcagtt
    21061 tgtcactgaa aaacgcaaag cgtcaatttc tggcgtacag cgtcagttcc gcattggtta
    21121 taaccgtgca gcgcgtatta tcgaacagat ggaagcgcag gggattgtca gcgaacaggg
    21181 gcacaacggt aatcgtgaag tgctggcccc accgccgttt gactaattaa tgcattgccg
    21241 gataaggcgc ggtagcgtcg catccggcac tctatcaact gaaaattcag tattttcttc
    21301 tttcctcaag ctgattatta gcctggaata gagagtagag ggaactcccg atcgggagtg
    21361 acgtaatttg aggaataatg atgaaaaaaa ttgctatcac ctgtgcatta ctctcaagct
    21421 tagtagcaag cagcgtttgg gctgatgccg caagcgatct gaaaagccgc ctggataaag
    21481 tcagcagctt ccacgccagc ttcacacaaa aagtgactga cggtagcggc gcggcggtgc
    21541 aggaaggtca gggcgatctg tgggtgaaac gtccaaactt attcaactgg catatgacac
    21601 aacctgatga aagcattctg gtttctgacg gtaaaacact gtggttctat aacccgttcg
    21661 ttgagcaagc tacggcaacc tggctgaaag atgccaccgg taatacgccg tttatgctga
    21721 ttgcccgcaa ccagtccagc gactggcagc agtacaatat caaacagaat ggcgatgact
    21781 ttgtcctgac gccgaaagcc agcaatggca atctgaagca gttcaccatt aacgtgggac
    21841 gtgatggcac aatccatcag tttagcgcgg tggagcagga cgatcagcgc agcagttatc
    21901 agctgaaatc ccagcaaaat ggggctgtgg atgcagcgaa atttaccttc accccgccgc
    21961 aaggcgtcac ggtagatgat caacgtaagt agaggcacct gagtgagcaa tctgtcgctc
    22021 gatttttcgg ataatacttt tcaacctctg gccgcgcgta tgcggccaga aaatttagca
    22081 cagtatatcg gtcagcaaca tttgctggct gcggggaagc cgttgccgcg cgctatcgaa
    22141 gccgggcatt tgcattctat gatcctctgg gggccaccgg gtaccggcaa aacaaccctc
    22201 gctgaagtga ttgcccgcta tgcgaacgct gatgtggaac gtatttctgc cgtcacctct
    22261 ggcgtgaaag agattcgcga ggcgatcgag cgcgcccggc aaaaccgcaa tgcaggtcgc
    22321 cgcactattc tttttgttga cgaagttcac cgtttcaaca aaagccagca ggatgcattt
    22381 ctgccacata ttgaagacgg caccatcact tttattggcg caaccactga aaacccgtcg
    22441 tttgagctta attcggcact gctttcccgt gcccgtgttt atctgttgaa atccctgagt
    22501 acagaggata ttgagcaagt actaactcag gcgatggaag acaaaacccg tggctatggt
    22561 ggtcaggata ttgttctgcc agatgataca cgacgtgcca ttgcagaact ggtgaatggc
    22621 gacgcgcgcc gggcgttaaa tacgctggaa atgatggcgg atatggccga agtctatgat
    22681 agcggtaagc gggtcctgaa gcctgaatta ctgaccgaaa tcgccggtga acgtagcgcc
    22741 cgctttgata acaaaggcga tcgcttttac gatctaattt ccgcactgca taagtcggta
    22801 cgtggtagcg cacccgatgc ggcgctgtac tggtatgcgc gaattattac cgctggtggc
    22861 gatccgttat atgtcgcgcg tcgctgtctg gcgattgcgt ctgaagacgt cggtaatgcc
    22921 gatccacggg cgatgcaggt ggcaattgcg gcctgggatt gctttactcg cgttggcccg
    22981 gcggaaggtg aacgcgccat tgctcaggcg attgtttacc tggcctgcgc gccaaaaagc
    23041 aacgctgtct acactgcgtt taaagccgcg ctggccgatg ctcgcgaacg cccggattat
    23101 gacgtgccgg ttcatttgcg taatgcgccg acgaaattaa tgaaggaaat gggctacggg
    23161 caggaatatc gttatgctca tgatgaagca aacgcttatg ctgccggtga ggtttacttc
    23221 ccgccggaaa tagcacaaac acgctattat ttcccgacaa acaggggcct tgaaggcaag
    23281 attggtgaaa agctcgcctg gctggctgaa caggatcaaa atagccccat aaaacgctac
    23341 cgttaatgtt atcgttgcgg taatgttgtt actgtatccc tgtggtcgca ggctgtggcc
    23401 acatctccca tttaattcga taagcacagg ataagcatgc tcgatcccaa tctgctgcgt
    23461 aatgagccag acgcagtcgc tgaaaaactg gcacgccggg gctttaagct ggatgtagat
    23521 aagctgggcg ctcttgaaga gcgtcgtaaa gtattgcagg tcaaaacgga aaacctgcaa
    23581 gcggagcgta actcccgatc gaaatccatt ggccaggcga aagcgcgcgg ggaagatatc
    23641 gagcctttac gtctggaagt gaacaaactg ggcgaagagc tggatgcagc aaaagccgag
    23701 ctggatgctt tacaggctga aattcgcgat atcgcgctga ccatccctaa cctgcctgca
    23761 gatgaagtgc cggtaggtaa agacgaaaat gacaacgttg aagtcagccg ctggggtacc
    23821 ccgcgtgagt ttgactttga agttcgtgac cacgtgacgc tgggtgaaat gcactctggc
    23881 ctcgactttg cagctgcagt taagctgact ggttcccgct ttgtggtaat gaaagggcag
    23941 attgctcgca tgcaccgcgc actgtcgcag tttatgctgg atctgcatac cgaacagcat
    24001 ggctacagtg agaactatgt tccgtacctg gttaaccagg acacgctgta cggtacgggt
    24061 cagctgccga aatttgctgg cgatctgttc catactcgtc cgctggaaga agaagcagac
    24121 accagtaact atgcgctgat cccaacggca gaagttccgc tgactaacct ggtgcgcggt
    24181 gaaatcatcg atgaagatga tctgccaatt aagatgaccg cccacacccc atgcttccgt
    24241 tctgaagccg gttcatatgg tcgtgacacc cgtggtctga tccgtatgca ccagttcgac
    24301 aaagttgaaa tggtgcagat cgtgcgccca gaagactcaa tggcggcgct ggaagagatg
    24361 actggtcatg cagaaaaagt cctgcagttg ctgggcctgc cgtaccgtaa aatcatcctt
    24421 tgcactggcg acatgggctt tggcgcttgc aaaacttacg acctggaagt atggatcccg
    24481 gcacagaaca cctaccgtga gatctcttcc tgctccaacg tttgggattt ccaggcacgt
    24541 cgtatgcagg cacgttgccg cagcaagtcg gacaagaaaa cccgtctggt tcataccctg
    24601 aacggttctg gtctggctgt tggtcgtacg ctggttgcag taatggaaaa ctatcagcag
    24661 gctgatggtc gtattgaagt accagaagtt ctgcgtccgt atatgaacgg actggaatat
    24721 attggctaat acccaatttt tctgaatcta aaaagcgcct ttagggcgct ttttttgtct
    24781 ccctttgata ccgaacaata attactcctc acttacacgt aatactactt tcgagtgaaa
    24841 atctacctat ctctttgatt ttcaaattat tctatgtata caagcctata tagcgagctg
    24901 ctatagaaat aattacacaa tacggtttgt tactggaatc aatcgtgatc aagcttgagt
    24961 gagccattat gaaaacgaaa atccctgatg cggtattggc tgctgaggag agtcgccgtg
    25021 gtttggtaaa aacgacagcg atcggcggcc tggcaatggc cagcagcgca ttaacattac
    25081 cttttagtcg gattgcgcac gctgtcgata gcgccattcc aacaaaatca gacgaaaagg
    25141 ttatctggag cgcctgtaca gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg
    25201 tggacggtga aatcaaatat gtcgaaacga caataccggc gatgacaatt acgacggcct
    25261 acactaggtt cgcgcctgcc tgcgtgggcg ttccatgcgt caccgtgtct acaatccgga
    25321 ccgcctgaaa tatccgatga aacgagtcgg ggcgcgcggt gaaggcaaat tcgagcgcat
    25381 tagctgggaa gaagcctacg acatcatcgc gaccaatatg cagcgcctga tcaaagagta
    25441 cggcaacgag tctatctatc tgaactatgg caccggtacg ctgggcggca ccatgacccg
    25501 ctcctggccg ccgggaaata ccctggtcgc gcgcctgatg aactgctgcg gcggttatct
    25561 taaccattac ggtgactact cttccgcgca aattgcggaa ggtttgaacc atacctacgg
    25621 cggctgggca gatggtaaca gcccgtcgga tatcgaaaac agtaaattgg tagtgctgtt
    25681 tggtaataac ccaggcgaaa cgcgaatgag tggcggtggg gtgacttact atcttgaaca
    25741 ggcacgccag aaatctaatg ctcgcatgat catcatcgat ccgcgctata ccgacaccgg
    25801 tgccgggcgc gaagatgagt ggatccctat tcgtccagga acagatgccg cactggttaa
    25861 cggtctggcg tacgtcatga tcactaaaaa cctggtggat caggcattcc tcgataaata
    25921 ttgcgttggc tatgacgaga aaaccctgcc cgccagtgcg ccgaaaaatg gtcattataa
    25981 agcttatatt ctgggtgaag ggccagatgg cgtggctaaa acaccggaat gggcctcgca
    26041 aatcaccggt gttccggcag acaaaatcat caaactggct cgtgaaatcg gcagtaccaa
    26101 accggcgttt atcagccagg gatggggccc gcagcgtcac gctaacggtg aaatcgcaac
    26161 ccgtgctatc tcgatgctgg cgattctgac cggtaacgtt ggtattaatg gagggaacag
    26221 cggcgcgcgt gaaggttcat acagcttacc gtttgtccgt atgccgacct tggaaaaccc
    26281 gatccagacc agcatttcga tgtttatgtg gaccgatgcc attgaacgtg gcccggaaat
    26341 gacggcgctg cgtgatggtg tgcgcgggaa agataagctg gatgtgccga tcaaaatgat
    26401 ctggaactat gccggtaact gcctgattaa ccagcattct gaaatcaacc gtacccatga
    26461 aatcctccag gatgataaga agtgcgagct gattgtggtt atcgactgcc acatgacctc
    26521 atcggcgaaa tatgctgaca tcctgctgcc tgactgcacc gcttccgaac agatggactt
    26581 tgcactggat gcatcctgcg ggaatatgtc ttacgtgatt ttcaacgatc aggtgattaa
    26641 accgcgcttt gaatgtaaga ccatctatga aatgaccagc gaactggcaa aacgtcttgg
    26701 cgttgagcaa cagtttactg aaggccgtac ccaggaagag tggatgcggc atctgtatgc
    26761 ccagtcgcgg gaagcgattc ctgaactgcc aacgtttgaa gagttccgca agcaggggat
    26821 ctttaaaaag cgcgacccac aagggcatca cgttgcttat aaagccttcc gtgaagatcc
    26881 gcaggcaaac ccactgacta cgccatcggg caaaattgag atttattcgc aggcgctggc
    26941 tgacattgcc gctacctggg aattgccaga aggcgatgtg atcgatccac tgccgatcta
    27001 cacgccgggc tttgaaagtt atcaggatcc gctgaacaaa cagtatccgc tgcagcttac
    27061 aggtttccac tataaatctc gcgttcactc aacttacggc aacgttgatg tgctgaaagc
    27121 ggcttgccgt caggaaatgt ggatcaaccc gcttgatgcc caaaaacgcg gtatccacaa
    27181 cggcgataaa gtcaggatct ttaacgatcg tggtgaggtt catattgagg cgaaagtgac
    27241 gccacgaatg atgccgggcg tggtcgcact gggtgaaggc gcctggtatg acccggatgc
    27301 aaaacgtgtc gataagggcg gttgtatcaa cgtactgacc actcaacgtc cgtctcctct
    27361 cgctaagggg aatccgtcac atacaaacct tgttcaggtt gaaaaggtgt aaggagtaac
    27421 cgatgacaac ccagtatgga ttttttattg attccagccg ttgcaccggt tgcaaaacct
    27481 gcgagctggc ttgtaaagac tacaaagatt tgacgccaga agtcagtttc cgccgcattt
    27541 atgaatatgc tggcggcgac tggcaggaag ataacggtgt ctggcaccag aacgtgtttg
    27601 cctactatct gtcgatttcg tgcaaccact gcgaagatcc ggcttgtact aaagtctgcc
    27661 cgagcggtgc gatgcataaa cgcgaagatg gttttgttgt ggtcgatgaa gatgtatgca
    27721 ttggctgccg ttactgtcat atggcttgcc cgtatggcgc accgcaatat aacgaaacga
    27781 aaggccatat gaccaaatgc gatggttgtt atgaccgtgt tgcggagggt aaaaagccga
    27841 tctgtgttga atcttgcccg ctgcgggcgc tggatttcgg ccctatcgac gagctgcgta
    27901 aaaaacatgg cgatctggcg gccgttgcgc cgttgccgcg agctcacttt accaaaccga
    27961 atattgtgat caaacccaat gccaatagcc gcccgaccgg ggataccact ggctatctgg
    28021 caaacccgaa ggaggtgtga gatgggaagt ggatggcatg aatggccgct gatgatcttc
    28081 acggtcttcg ggcaatgtgt ggcgggtggt tttatcgttc tggctttggc gctgctcaaa
    28141 ggcgacctgc gagcagaagc ccagcagcgt gttatcgcct gcatgtttgg cttatgggta
    28201 ctgatgggca ttggctttat cgcctctatg ctccatcttg gttcaccgat gcgcgccttt
    28261 aactcgctca accgggtagg ggcttcagca ctcagtaacg aaatcgccag cggttcgatc
    28321 ttttttgctg taggcggcat cggctggttg ctggcaatgc tgaaaaagct gtcaccggca
    28381 ttgcgtacgc tatggctggt ggtgacgatg gttcttggcg tcgtctttgt ctggatgatg
    28441 gtgcgtgtgt ataacagcat tgataccgtt ccgacctggt acagcatctg gacgccgatg
    28501 ggctgggcga tgcgtctgct gccagccatt tctgtactgg cactggtggt gagtggcgtg
    28561 atgtcagtga tgcagggcac agagctggca actattcata gttctgtgca gcaggccgca
    28621 gcgctggtgc cggactatgg tgcgctgatg tcctggcgga tcgtgctttt ggccgttgcc
    28681 ctgtgcttgt ggattgcacc acagctaaaa ggttatcagc ctgcggttcc gctactatcc
    28741 gtctcattca ttctgcttct ggcaggggaa ttaatcggtc gcggcgtatt ctatggtttg
    28801 catatgaccg tggggatggc cgtcgcaagc taatcagtaa caaccggggt ttcggccccg
    28861 gttctctttt atttctgctt cgttaacgtg tcataactgg tcatcaaatt acgataatca
    28921 ggaatatggt tagagaacaa cgtcgccagt ccttcaatat cattacgcca gtcacggtgc
    28981 agctcgcaag ccacgccgaa ccatgtcatc aattgcgcac cagcttgcga catgcgatcc
    29041 cacgccgaat gccgggtaat ttcattaaag gtccctgatg catcagtcac gacaaagaca
    29101 tcaaaccctt cttcaatggc tgaaagcgcc gggaatgcca cgcaaacttc ggttaccaca
    29161 ccggcaataa ttaactgttt tttacctgtc gctttgactg cttttacaaa atcttcgtta
    29221 tcccaggcgt taatattgcc cgggcgagca atgtaaggtg catcggggaa ttgtgctttc
    29281 agttctagaa ctaatgggcc gttaggaccg gtttcaaaac tggtggtaag aatagttggt
    29341 aagttgaaat acttagccag gtcacccagc gccagcacgt tatttttaaa cttatcgggt
    29401 tcgatatccc gtacaaggga aagtaaacca gcctggtgat caacaagcaa aacggcagca
    29461 tcatttttat caagacgaac atacggtttg gtcatcctct tctcctttcg agagtcagtg
    29521 gtacgtctga aatcggtcag acgcgatgca ttgctctgaa agcatagacg ggaaatatga
    29581 gtttgctgtg accatgaaat ttttcgactg aacgaagcgt atgaaatttg tgttagatca
    29641 ataaaaacaa tcagatgaga ttaatcatct cttcagcatt agcgttatga atgagtaata
    29701 taaatcgata ataccgccgc tgcgatatgc gcgtattttc acccattgac aatgtttttg
    29761 gcggtggcat gatgcgcatg aaatttgaac ttcctcacgg ttttaattca tgtccacgta
    29821 tacccggcct gtcatgcttt tgctgtctgg cctgcttttg ttgactctgg cgatcgcggt
    29881 gttaaataca ctcgtgccgc tttggctcgc ccaggaacac atgtccacat ggcaggtagg
    29941 cgttgtcagc tcatcctatt ttaccggcaa ccttgtcggt acattgctga cagggtatgt
    30001 cattaaacgc attggcttta accgcagcta ttatctgtcc tccttcattt ttgctgctgg
    30061 ctgtgccggc cttggcctga tgattggttt ctggagctgg ttggcgtggc gttttgtcgc
    30121 gggcgtcggc tgtgccatga tttgggtggt tgttgagagc gcgctgatgt gcagtgggac
    30181 gtcacgtaac cgtgggcgtt tgcttgctgc gtatatgatg gtttattacg tgggaacgtt
    30241 tttaggccag ttactggtca gcaaagtctc aaccgagctg atgagcgtat tgccgtgggt
    30301 tacaggtttg acattggcag ggatcttacc gctgttgttt acgcgtgtcc tgaatcagca
    30361 ggctgaaaac catgattcga cgtcaattac ggcaatgcta aaactccgtc aggcgcggct
    30421 tggcgtgaat ggctgcatta tctcaggaat cgttctggga tctctatatg gcctgatgcc
    30481 tctgtacctc aatcacaaag gggtgagcaa tgccagtatt ggtttctgga tggcggtact
    30541 ggtcagtgcg ggtatcctcg gacaatggcc gattggacgt ctggcggata agtttggtcg
    30601 actgctggtt ttgcgtgttc aggtctttgt cgtcattctc ggcagtattg cgatgcttag
    30661 ccaggcggcg atggccccag cgttattcat cctcggtgcc gctggcttta cgctatatcc
    30721 ggtggcgatg gcctgggctt gtgagaaagt tgaacatcat caactggtgg cgatgaacca
    30781 agccttactg ttgagctata ccgtgggaag tctgcttggc ccgtcattta gcgctatgct
    30841 aatgcagaat ttctccgata atttattgtt tatcatgatc gccagcgtat cgtttatcta
    30901 tttgctgatg ctgcttcgca acgccggtca tacgccgaaa cccgttgctc acgtgtaaat
    30961 gaattcaagc aaagtgtgaa cttactgttt cacactctgc ttttttgttt cttctatctg
    31021 acttgctttt ttccaaattt tattcgttta aaaataaaat gtgcagcagg ttataatttt
    31081 gcatttcgct atttccgcac ttcttatttg ccgcgcataa tccctcgttt tacagatgcc
    31141 cctttaattt tggcgaagga tttgtctatg gctgggaatg ttcag
//
LOCUS       DAJTSI010000033        31129 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000033 DAJTSI010000000
VERSION     DAJTSI010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31129
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.033"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="OO327_001351"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="OO327_001351"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR6017956.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="OO327_001352"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="OO327_001352"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR6017957.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="OO327_001353"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="OO327_001353"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR6017958.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="OO327_001354"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="OO327_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR6017959.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="OO327_001355"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="OO327_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR6017960.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /gene="radC"
                     /locus_tag="OO327_001356"
     CDS             complement(3426..4094)
                     /gene="radC"
                     /locus_tag="OO327_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR6017961.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="OO327_001357"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="OO327_001357"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR6017962.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="OO327_001358"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="OO327_001358"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR6017963.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="OO327_001359"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="OO327_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR6017964.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="OO327_001360"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="OO327_001360"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR6017965.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="OO327_001361"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="OO327_001361"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR6017966.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="OO327_001362"
     CDS             8212..9075
                     /locus_tag="OO327_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR6017967.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="OO327_001363"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="OO327_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR6017968.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="OO327_001364"
     CDS             10411..11028
                     /locus_tag="OO327_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR6017969.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="OO327_001365"
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="OO327_001365"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR6017970.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVLQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="OO327_001366"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="OO327_001366"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR6017971.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="OO327_001367"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="OO327_001367"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR6017972.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="OO327_001368"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="OO327_001368"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR6017973.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="OO327_001369"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="OO327_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR6017974.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="OO327_001370"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="OO327_001370"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR6017975.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="OO327_001371"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="OO327_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR6017976.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20347..21738
                     /gene="xanP"
                     /locus_tag="OO327_001372"
     CDS             20347..21738
                     /gene="xanP"
                     /locus_tag="OO327_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR6017977.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     SSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21859..23568
                     /locus_tag="OO327_001373"
     CDS             21859..23568
                     /locus_tag="OO327_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6017978.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="OO327_001374"
     CDS             complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="OO327_001374"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR6017979.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25949..27332)
                     /locus_tag="OO327_001375"
                     /pseudo
     CDS             complement(25949..27332)
                     /locus_tag="OO327_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27625..27719
                     /locus_tag="OO327_001376"
     tRNA            27625..27719
                     /locus_tag="OO327_001376"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27659..27661,aa:Sec,seq:tca)
     gene            27991..29202
                     /locus_tag="OO327_001377"
     CDS             27991..29202
                     /locus_tag="OO327_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6017980.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
     gene            29614..30657
                     /locus_tag="OO327_001378"
     CDS             29614..30657
                     /locus_tag="OO327_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR6017981.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
BASE COUNT         7791 a         8226 c         7719 g         7393 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcacgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccaca cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatgattt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcaa
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcagcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgaatggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtactgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa
    20221 ataccaaaag ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg
    20281 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    20341 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa
    20401 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc
    20461 gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    20521 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    20581 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg
    20641 tctattcagg gctccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg
    20701 aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg
    20761 gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    20821 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg
    20881 acctccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat
    20941 ctgctgctgg caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct
    21001 tacttacgcg tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt
    21061 atgggcatgt tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg
    21121 ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt
    21181 atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag
    21241 ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg
    21301 aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac
    21361 ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
    21421 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
    21481 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
    21541 gtttctcgtg agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt
    21601 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
    21661 ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
    21721 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
    21781 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
    21841 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
    21901 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat
    21961 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
    22021 cgtttttccg cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag
    22081 cccgcgcccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa
    22141 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
    22201 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
    22261 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca
    22321 gaagccggta aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg
    22381 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
    22441 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
    22501 aacgccgacg gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc
    22561 gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt
    22621 cgcttggaag tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat
    22681 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg
    22741 tcgatgaacg ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac
    22801 gcggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt
    22861 atggtcagaa cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg
    22921 gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg
    22981 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
    23041 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac
    23101 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
    23161 gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac
    23221 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
    23281 accgcaagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt
    23341 gtgccggtga atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc
    23401 aatattcagc ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt
    23461 agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg
    23521 ggcgtcgttt ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg
    23581 ccgggtacga ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg
    23641 cattcccttg aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca
    23701 gaccatttac tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac
    23761 cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt
    23821 cagcagcagg cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg
    23881 ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag
    23941 tgttatcacg cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac
    24001 taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact
    24061 gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt
    24121 gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca
    24181 tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc
    24241 atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca
    24301 cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc
    24361 agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga
    24421 agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt
    24481 agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg
    24541 agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca
    24601 gttcgttgta gatgtacgca taatggttat gcattttctg cgaatcggaa ccgtcaaacc
    24661 actgaacatc aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg
    24721 cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat
    24781 aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga
    24841 gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt
    24901 taatccagac gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt
    24961 cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac
    25021 agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt
    25081 ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc
    25141 acgggggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg
    25201 gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt
    25261 tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc
    25321 cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc
    25381 cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct
    25441 ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc
    25501 gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt
    25561 cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt
    25621 tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca
    25681 aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca
    25741 caccgacaat accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct
    25801 gccaggtacg ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct
    25861 gttcaacctc gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt
    25921 ttccatcgct aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt
    25981 gctggcaacg gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg
    26041 tgagtgagta gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa
    26101 tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat
    26161 catatccgcc ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt
    26221 tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact
    26281 cgccgtagtc gacggtatcg aacatcatta cccactggat aggtgtcacc agttgatgca
    26341 acacaccaat cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga
    26401 acatcgcgag gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac
    26461 atttccagtc ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag
    26521 tggtgagaaa agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca
    26581 tcgccccacc gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc
    26641 gccactggtc gttttgccag atatcacgta aatcttcccg catagacgtt gttgtaggtg
    26701 gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca
    26761 cggaaaggac cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat
    26821 taaccagtgg catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac
    26881 gccaggattg cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc
    26941 agtaagggat attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca
    27001 tagatcattt tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc
    27061 ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt
    27121 cgatcggcca acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa
    27181 aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata
    27241 tataacatta cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt
    27301 ttctctttaa cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc
    27361 tgagaccgat aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt
    27421 tattaattta tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca
    27481 ttgtaaaata tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt
    27541 aggcggaaaa aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc
    27601 gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc
    27661 aaatccagtt ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag
    27721 tttacctctt ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt
    27781 atttatttgt atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac
    27841 tttagggggt gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata
    27901 cataacaacg gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt
    27961 tagttactgg cagaaatccc cctcaggaac atgaattcat caaaagccgg aggatgtcat
    28021 ggcattaacg atatcaaagt gaaaaccgca aaacccaaag acaaacccta taagcttgct
    28081 gatggcggcg gtatgtatct actgattaac actaatggtt ctaaatactg gcgcatgaag
    28141 taccgctttg ctggtaaaga aaagatgctt tctattggcg tataccccga tgtaacgcta
    28201 gccgatgctc gcgagaaacg cagtgaagcc aggaaactcc tcgctgctgg aggcgatcca
    28261 ggggaggcaa aaaaggaaga aaaaatcgcg cagcaaatga gtctgaaaaa tacgttcgag
    28321 gctattgctc gcgaatggca ccagttaaaa gctgatcggt ggtcattacg ctatcgtgat
    28381 gaaatcattg atacctttga aaaagacatt tttccctaca ttggcaagcg ccctattgcc
    28441 gaaatcaaac caatgaagtt actggaagca ctgcgtaaaa tggaaaaacg tggagctctg
    28501 gagaaaatgc gcaaggtccg ccagcgctgt ggtgaggtgt tccgctatgc aattgttact
    28561 ggccgggcag actataaccc agcccccgat cttgccagtg ctttagctac accgaaaaaa
    28621 gtacattttc cctttcttac tgctaatgaa cttccccact tcctcaacga tctggcgggt
    28681 tataccggaa gtataatcac taagacagct actcagatca ttatgttaac cggcgtacga
    28741 acacaagaac tgcgttttgc acgctgggaa gatatcgatt ttgagacaaa attgtgggag
    28801 atcccggcag aagtaatgaa gatgaagcga cctcatattg tgccgctctc tgagcaggtc
    28861 attatgctat ttaagcagct tgaaccaatc tcaaaacatc atcccctagt ctttatcggt
    28921 agaaatgatc cccgcaaacc aattagtaag gaaagcatta accaagtaat tgaattactt
    28981 ggatataagg gaagactcac agggcacggt ttcagacaca ccatgagtac aattctgcat
    29041 gaacaaggct ttaattccgc ctggattgaa atgcagttag ctcatgtgga taaaaattcc
    29101 atcaggggta cctataatca tgccctgtat ctcgatggcc gccgtgaaat gatgcaatgg
    29161 tacgctgatt acattgattc actctccagc cgggaaagtt aagtcagaaa tacttttccc
    29221 tgatgttttc ctcaattaag tattttatcc tctggcctac aaggctggag gatttttgtt
    29281 attatattat ttttttgcgt cttcaaactt attacaaaaa cataattaaa aacaaattga
    29341 catttttaaa ttggcaatta caaaatacag ccttgcagtt atttcgatac ctttagcaaa
    29401 ggaatagttg attcagatat aatttagcat ttcaatcatc tgacatcatt ataaaaaatg
    29461 agattcacat aaagataaaa ttattgttct caaggaaagc atgaaggggc ttcgtaagca
    29521 atattcattc ggtgccaaga caggacttga tacacagaat ctcaactttt atgctctcag
    29581 actatatcac cacatgtttt acaggctcag aaaatgaacg atagattatg ctttgaagtg
    29641 cacgataaca agggttattt cgtatttccg gatacctggt ttggcccatt gctgggggag
    29701 tttgaagaag tgcttgatgc atacgacgct gatgaaatca gcgagacgag ttacatcaac
    29761 aaactccgac gccttgctca acgggagccg gactttattg atatacacgc tcatctggca
    29821 tatgcatttc tggaacagaa tgccccgcgt aaggcactga acgccgctct taaaggactg
    29881 gcggcaggta atcgtatcat cccggaaagt ttctgtggtg aaataatctg gatgcatccc
    29941 gaaaaccggc cttatctgcg cgcgctttat gcagcaattc tcgcaaatgt gcacttacag
    30001 cgccatcagg atgcggttat gctcactgat aaaatactgg catacaatcc tgaagataat
    30061 cagggggctc gctggttgtt gggttcagag ctgttacgca ccggcgatca tgagcgagcc
    30121 ttcagtgtac tgaaagagca tgctgatgaa ttttcacctt actggtatga actcggtctc
    30181 ctgcattttc tgaacgggga acatgttaag gccgccacag cttttcgcca tggctttgcc
    30241 acaaacactt acatcgcaga aatgctgtgc ggaaatctgc atccatttcc acttgctgtg
    30301 tggcatgact tctccggctc tctggatacg gcagaagatt actatgcaac atactcacca
    30361 ttatggggac aatatccgga agcattgctg tttgtaaact ggctctataa ccattcctcc
    30421 gtgctgcatg aacgatcaga aatcattaaa tgcgccgaaa tgctgattca ggaagatgat
    30481 ttcgaaatat gcgaaagtat tttaagacag caggagcacc tgtggaaaag aattgacaag
    30541 acactttctg aggaaattgt acagaaatgc agaaatatga acggtgaata tatctggccc
    30601 tggatattgc ccttttccgc agcagggatg aaacattcaa gcatccagta tcagtagata
    30661 ttgcattagt gtaactttaa aacaagtaat aatttattat ccaataataa cactgtgttc
    30721 cttccccagg tgattaccct ggggttattt tctgaaagtt tcctttgcaa aaggatagcg
    30781 ttgtaaatta ctttaacgaa ttactccatg aactcattaa agttctttcg taatacttta
    30841 tcgtcaaaca cgttatagct ggcatcaaaa accacagatt taccctgcca gcgggtaatc
    30901 ccctcctctg gcgttttcgg ttgaactggt atccccgtcc aacacaggca gccattataa
    30961 acagtaaaaa attgtattcg ctgttatgaa atcaatagtc ctgacgtact gttgaatgtg
    31021 cttgtgttct gaacctcgta caaccagtac atttgatatc gaattataaa caaaacattg
    31081 catccaactt ttttaatatg gacaatagtt gtccatacgg tggttttat
//
LOCUS       DAJTSI010000034        30295 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000034 DAJTSI010000000
VERSION     DAJTSI010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.034"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..815)
                     /gene="wcaD"
                     /locus_tag="OO327_001379"
     CDS             complement(<1..815)
                     /gene="wcaD"
                     /locus_tag="OO327_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR6017982.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
     gene            complement(790..2007)
                     /gene="wcaC"
                     /locus_tag="OO327_001380"
     CDS             complement(790..2007)
                     /gene="wcaC"
                     /locus_tag="OO327_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR6017983.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2004..2492)
                     /gene="wcaB"
                     /locus_tag="OO327_001381"
     CDS             complement(2004..2492)
                     /gene="wcaB"
                     /locus_tag="OO327_001381"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCR6017984.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2495..3334)
                     /gene="wcaA"
                     /locus_tag="OO327_001382"
                     /pseudo
     CDS             complement(2495..3334)
                     /gene="wcaA"
                     /locus_tag="OO327_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3427..5589)
                     /gene="wzc"
                     /locus_tag="OO327_001383"
     CDS             complement(3427..5589)
                     /gene="wzc"
                     /locus_tag="OO327_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCR6017985.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5592..6035)
                     /gene="wzb"
                     /locus_tag="OO327_001384"
     CDS             complement(5592..6035)
                     /gene="wzb"
                     /locus_tag="OO327_001384"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCR6017986.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYHKSRETFAAVHTLLERSARQWAQSLNAEQV"
     gene            complement(6041..7180)
                     /gene="wza"
                     /locus_tag="OO327_001385"
     CDS             complement(6041..7180)
                     /gene="wza"
                     /locus_tag="OO327_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCR6017987.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7489..7638
                     /locus_tag="OO327_001386"
     CDS             7489..7638
                     /locus_tag="OO327_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6017988.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7839..9422
                     /locus_tag="OO327_001387"
     CDS             7839..9422
                     /locus_tag="OO327_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6017989.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9874..11727)
                     /gene="asmA"
                     /locus_tag="OO327_001388"
     CDS             complement(9874..11727)
                     /gene="asmA"
                     /locus_tag="OO327_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR6017990.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11749..12330)
                     /gene="dcd"
                     /locus_tag="OO327_001389"
     CDS             complement(11749..12330)
                     /gene="dcd"
                     /locus_tag="OO327_001389"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR6017991.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12422..13063)
                     /gene="udk"
                     /locus_tag="OO327_001390"
     CDS             complement(12422..13063)
                     /gene="udk"
                     /locus_tag="OO327_001390"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR6017992.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13381..13623
                     /locus_tag="OO327_001391"
                     /pseudo
     CDS             13381..13623
                     /locus_tag="OO327_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            13622..14563
                     /gene="mdtA"
                     /locus_tag="OO327_001392"
                     /pseudo
     CDS             13622..14563
                     /gene="mdtA"
                     /locus_tag="OO327_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
     gene            14563..17685
                     /gene="mdtB"
                     /locus_tag="OO327_001393"
                     /pseudo
     CDS             14563..17685
                     /gene="mdtB"
                     /locus_tag="OO327_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            17686..20751
                     /gene="mdtC"
                     /locus_tag="OO327_001394"
     CDS             17686..20751
                     /gene="mdtC"
                     /locus_tag="OO327_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR6017993.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            20752..22167
                     /locus_tag="OO327_001395"
     CDS             20752..22167
                     /locus_tag="OO327_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6017994.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            22164..23567
                     /gene="baeS"
                     /locus_tag="OO327_001396"
     CDS             22164..23567
                     /gene="baeS"
                     /locus_tag="OO327_001396"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR6017995.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            23564..24286
                     /gene="baeR"
                     /locus_tag="OO327_001397"
     CDS             23564..24286
                     /gene="baeR"
                     /locus_tag="OO327_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR6017996.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            24477..24809
                     /locus_tag="OO327_001398"
     CDS             24477..24809
                     /locus_tag="OO327_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR6017997.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            24956..26317
                     /gene="yegQ"
                     /locus_tag="OO327_001399"
     CDS             24956..26317
                     /gene="yegQ"
                     /locus_tag="OO327_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR6017998.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(26659..26976)
                     /locus_tag="OO327_001400"
     CDS             complement(26659..26976)
                     /locus_tag="OO327_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6017999.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            27382..28281
                     /gene="yegS"
                     /locus_tag="OO327_001401"
     CDS             27382..28281
                     /gene="yegS"
                     /locus_tag="OO327_001401"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCR6018000.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(28363..29136)
                     /locus_tag="OO327_001402"
     CDS             complement(28363..29136)
                     /locus_tag="OO327_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6018001.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(29242..30282)
                     /gene="gatD"
                     /locus_tag="OO327_001403"
     CDS             complement(29242..30282)
                     /gene="gatD"
                     /locus_tag="OO327_001403"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6018002.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMQGKVLLIP"
BASE COUNT         7009 a         8046 c         7830 g         7410 t
ORIGIN      
        1 cctaaatcgc ccagacgggt ggaaatataa ggaaacgcga ttaccacacc aacccggaat
       61 acagccagag aaatcaatgc taacggtaat tttttcttaa tcgcctcttt attcagatat
      121 tgaaatgccc actccagcaa ataaaacagg ataaaggtca taacccctga aaacgatccg
      181 gataatatta tccctgcgag aatcagggca tctgttttag gcgttttgat accaaactgt
      241 ttgatgctga gccaaattga gattaatgcc agagcgaaaa atgccggttc gaaataaagt
      301 gctgtcgtgc gcttgccgcc gaatttaatg aaattcagca catagctgtt gctgtaaatc
      361 agatatttcg aaatcgactc cataatactg ctgccaccgg tgaggataat ttgcgccatc
      421 tccaccgccg ccagcgccac caccagtccg accaccagat aaaagaaacg taatatctta
      481 cgatggttgt gtggcgaaat tgttttaaaa cgaatactcc acaccatgcc aataatgatc
      541 acaatataga caaacagcat agttgaagtg acgtatttac cggcatccaa cgactgacca
      601 aacaggtagt taaacgccgt gagtcccgcg ccaatcccta aggcaatcat caatttttta
      661 atgctgatgc gttctaaaaa cagcagtaac aagacaggta aaaaagtgac gatggtgatg
      721 gggaaacttt cgccaagctg ggcgattttg acgttaacca gcaggtagat cagtggcagc
      781 agcaggtagc tacagattct gatagaagtt gacatactcc tccagcatct gttgtccact
      841 gtaggcggcg cgactgcgtt ggctgaactc agccagcgtg gtaccaaata tcgcctgcgc
      901 gatttccggt ttgcttaact gtaccagttg cagcacctct tcttcactga cggttttacc
      961 accggatttt tgcagcactt cccgcgccgc atcgctatgg gtggcaatca ccggcacgcc
     1021 aatcgatagc gcctcacaca aaatcagcgg gtagttatcg acacgagaac tgaacaccag
     1081 cgcatccatc tgattgagcg cgctcatcaa cttgcgcttg tcagtttcaa agccgtgatt
     1141 aaccacgttg ccagcggtga acggcgagaa cttaccaaag gtatgcagtt cgattttgtc
     1201 gcccagcgcc atcatctcac gcaccagttg ctggttagtt ttgccgtcgt aacgcaggtc
     1261 atgcgccacc actgcgattt tcggcttgcc ctgagtttcg cgcaccggag gcagatctgc
     1321 cagaatcgct tcggttgcca tatcaatgcc attattgata atccggcaac gccctggccc
     1381 gtacagacta ttgaaagcgt cagccacatg ctggctgggg gaaataaact gacagcccag
     1441 cgccagcatc tcgcggaata actggcgttt gcccgccacc agctggtgcg cgcgatcaat
     1501 cttcaccggc ggatagttat ttaaggtcgg gcatttctgg cagcccgttt tccagccttc
     1561 gcaaccgtcg gtaaaggcgc agcgtccggt tacactccag tggtcgtgca gcgtccagac
     1621 cagggtgaca tccggtttat ggtttttcac tttttcgcaa aagcgcacca cgctgttaag
     1681 attcaaccag tagctgtgca gcacatgaaa atgcaggacc accggacccg gtgtgcgagt
     1741 aatggtgcga tataactcat tgaaattgcc gaacagatcg cgattaaaca gacgaaacaa
     1801 cgcaatgttc gccatcgcgg tcatccgcgg cgtatgtttg atgacctgcg gatagttctg
     1861 atggctgacg ctctctttgc cgcctttgcc gtagccgtag acaaaatgtg acgccagccc
     1921 ctgttgcagc gcacgctggt ggagatctaa cgccacacct gctgccccgc cttccgccag
     1981 tcgcacatta aattgcagaa tattcattta atcaccttca ctcgcgcttt ttctcccacc
     2041 accagcgcgt tgtccgggac agagtcgagc accacgctgc ccgcacccac ggtgacgttg
     2101 ttaccaagcg tgatatcacc aagaataatg acgttggcac cgagttcgac gccgttgcca
     2161 atgtgtggac atgccatgtt atcggcaccc cgattgccga tagtgacgcc gtggcgaatg
     2221 gtgaaatcat cccccgccac cacgttttta ttgatcacta cggcgtaacc gtgatggata
     2281 gtaaagcggc ggccaatggt cgcggcggcc tggatttcat aaccgaaaaa gcattcggtg
     2341 ataatgcgat acagcaccag cagcggggcc gcccacagat tgttgaggac gttctttttg
     2401 cgccacaccg aacaaaaatg agcgacgcga taggcaagta ccatgcagca cgggcgtaaa
     2461 ctccagctgt tggcgcgcag atcttccagc atctttagcg cccccgaata ccatcagcca
     2521 gacgtttgcc gttacgcacc gacagcagcg tcaacaaggt gcgccaggtc atacgtttat
     2581 tgcggatctg gtagagggta aacagctgat attttttact ggcgcggtcg aatttgtctt
     2641 tgtgcttgcg gtaaaagtgg aagtatccgg agaatttttt cggtgacgag gtgatctgca
     2701 tttcgccgtg attgatatgc aggatctgcg tcgcctcttc cactttccac ggttcgccgt
     2761 actccaccac catccgcagg aatatgtcgt aatcctgtgc tgctttcagt tcggtatcga
     2821 acaaacactc tttgaaacgc catgcccagg taaagacctg attgccaatg atattacgtt
     2881 tatagaacag gcggcgtgag tacggcgatt tgggatacag tggtaagctc gccggttggg
     2941 aataaacttc tccctagcaa acgtagtcgt tagcgtacaa aaaggcgtgt gtgaccagtt
     3001 gctgtttatg ggcgaggaag acgctcaggc ggttgggcgt ccattcatca tcatcatcga
     3061 tcccggtgat gtattcccct tgcgccagca taatcgcctg gttacgcacc gcgcacgccc
     3121 cgctgttaat gtcgttgtgg atgtaagtga tacgcggatc gttgagggca gtgacgtact
     3181 gttgcagttg ttcccaggag gtggagcaat catccacgat gatcatctcc cagttgctgt
     3241 agtcctggcg caggaccgat tttatcgccc gaatcgccag ttgttggcgg ttccaggttg
     3301 gcatatagat tgagatcagc ggattgtctt tcataggttg ttctcccggc ttatgagctg
     3361 gtttgcttgc cggatgcggc gtgagcgcct tatccggcct acggggcggt gcgaatgcag
     3421 gcgtcgttat ttcgcatccg acttatattc gtattcgtaa tagccataat cctgatacgc
     3481 gctggcgcgg cggaagatgg agttcagaat cacccctttc accggaatac cgttttgctc
     3541 aaagcggctc agactggttt ccacttcttt caatgtgttg accgcataac gcgccaccat
     3601 taacgtggtt ccgacatgac gaccaacaat tgccgcatcg gtcactgcca gaatcggcgg
     3661 cgtatcaatc aacaccaggt catagttttt actcgcccag ttcaccagtt cggcaaagcg
     3721 ttcgctcatt aacagttcag aaggatttgg cggcacctga ccgcgcggga tcaggtcaaa
     3781 tttggcaata gaggtcggtt tagcagcggt agtaatatcg ccctgaccaa tcagaatctc
     3841 cgacagccca ttgacattat tggtgcccaa cagctcgtgg gtgtagcctt tgcgcatatc
     3901 gcagtcgatc aacaacacgc gtttattggt ctggctgatc accgccgcca ggttggcgca
     3961 gacaaaggtt ttaccgattg acgggctaac cccggtcatc atcaacacat tgttctgcgc
     4021 ctgcatcatc gcgaagtgca gactggtacg cagactacgg atggcttcaa tcgccagatc
     4081 ggttggattc cccactgcca gtagctggct ctgtttatag cgtttaaccc ctttgatggt
     4141 tttgacgcta tcgcgcgctt tctgccactc cgacagcggg atgctggcat agacgctgat
     4201 tccgtgttct tccagcactt gcgggctttc gatgccgcga ttaaacaacg agcgcagcag
     4261 cacacccacg atagagagca tcaggccaag gataatcgcg ccgaggataa tcagcccttt
     4321 cttcggtttt agcacgccag gctgggtgat tgccgggtca ataatgcgca catcgccgac
     4381 ggtgctggct tcggtgattt tcagctcctg ctctttattc agcagttgca tatagacctg
     4441 ctgaccagac tcgacatcgc gggtcagacg gacaatttcc tgctggtttt tcggcatcgc
     4501 cgttacgcga ccgttaagtt tggctttctc ttcttccagc gcctgacgtt tctccagcag
     4561 cgtgcggtac gccgggtgaa ctttggtgta cagcttggag atttccgcct ctttaaaggt
     4621 cagttcgttc aactgcgcgt cgatgttcac catcgaatcg agcaccgctt tcgcttcaag
     4681 cggcagatca acagaatctt tatcctgacg gaaggcattc agtttgtttt cggcaacatc
     4741 gaggcggcta cgtacttccg gtaactgttg cgcgaggaaa gcgaggcttt tcgacgcttc
     4801 cgccgatttg cgctcaatat tttgttcctg atagttacga gcgatgctgt taagaatgtc
     4861 gcggatctgt tcgcgatctt caccggtata agtcaggctc agtacgcctg cgtctttgcc
     4921 gttctccgtt accgtcaggc tgttttgcag ttggttgatc atccccaacg tggagtattt
     4981 ggtgacggta aactcactgc ccgggcgggc gtgaatggct tcaaccatca gcgtgacgcc
     5041 ttcttttttc agcatctggc ccgcttgccc acgggcgcta aagccgccat cactgctcag
     5101 ggtgtagttt ttgtcatcca gcacattaag cgtaaacacc tgatccgcca tctctttcgg
     5161 gcggttaaag gtagtcactt tcaccgtctc gttctgacgc cccatcaggc gatcccagcc
     5221 cgccccgaaa atcgggaacg tgtttttgct caccgcaata tcgaggtcga gatcgtccac
     5281 cgttttacca agcaccagac gcgaacgaat caactggatc tcggcgtccg atgcaggcgg
     5341 tttgttggct aatgccgagc cgatatcctg caccagcgaa ttgccgctgt tttgctcgat
     5401 ttgtaccagt gcgtcggcac tataaatcgg cgtggcgaag aaagtgtaaa ccacggcaca
     5461 gagggcaaat acgacggtga tgccaatcac ccaccagcgc gcttcaatga cggtgccgac
     5521 caggcgacca atatcgattt catcactgcc cgttaccgga gcggcatgtt gttttacttt
     5581 ttctgtcatt cttatacctg ctctgcgttc aatgactgcg cccactggcg ggcagaccgt
     5641 tcaagtaatg tgtgcaccgc tgcaaacgtt tcccggcttt tgtgatacgg atcggggatt
     5701 tcacattcgt tatcccagtg accaaacagc atcactttgc cgcgcatctc cggtgccatc
     5761 tcgcataagc gttcgatatg gcgcttatcc atggtcaaaa tcaggtcata gttgcgacac
     5821 aggcggcggc tgatttgtcg tgcacagtgg ccttccagag acagttgatg ttctgcggcg
     5881 acgctgatag cggtaggatc agcgccctta ccgaccagcg cgccgagtcc agcggactcc
     5941 actttcagtt ccgggtgata acgttgcagt aagcgttccg ccatcgggga acggcaaata
     6001 ttgccgacac agacaactaa gatgttgtta aacatgacga ttaccagtta tgaatgtcgc
     6061 tggctgtatc cgtcatgtaa cggacaccgc taatagttgg cagcaactga ttgatcagac
     6121 ggttccagcg ggaaaccggg gcggtggtga catacaccac gtcataaggt tgcaggcgga
     6181 attctgtcgc catcaccagc gacgtcgcat cggacatatc cagctggtag atattggcaa
     6241 tcttgccgtt acgcccgccc tcgcctttca gcggacgaat gacaaagatg ccgctggcgt
     6301 tggaggtggt catgtcgata ccttcggcat tggccagagc ttcagtcagg gtcatgccgc
     6361 taaagtccat tttcagggtg ctctgtttct tcacttcacc catcacaaat actttcagat
     6421 catcattacg cggcacgtag agaatatcgc cgggataaag cagtcggttc tgattgaggt
     6481 cgccgttttg catcagcgcc tgtaaagaaa tgcgttcttc acgaccattg tgtgttagca
     6541 ccacgttgcg ccagtcagcg gtgtcggtca ggccacctgc ggcgttgatg gcgtcgagaa
     6601 tggtcagtgg tacgttggtg atcgcctgtt gaccggattt attcacctga cctgagatat
     6661 aggccttttg tgagcggaag gcggcgatat taacgtccac ctgcgggtca gcgatgtacg
     6721 tcgctaagcg cccggtaata tcactgcgga tttcagcgag cgttttcccg actacgtgaa
     6781 ccttgccgat atacgggtaa aacatagtgc cgtcaggctg tacccagttg ccggtgtcgc
     6841 tggagctgcg gtactgacca gctggcgtgg tgagttccgg gtgatcccag acggtgacat
     6901 taagaacgtc ccccggcccg acgcgatact ggtaattcgc gatctcgctt tccagcgtca
     6961 tattggggcg cgctacattc gggcgtgggc gtaattggtc aatcaggcgc ggggtcagcg
     7021 gataaacatt caccattttg tcgagatcga aatcagcgtc ctgctgtttg atgacgtctt
     7081 tgcccatcgt cgacatattg ctgcccggaa gtactgtgca accgcttatc aaggttactg
     7141 acaccaataa tggcatcaat ttcattttgg atttcatcat tgtttattta tcactttggc
     7201 agagtaatta tcctgtgcac tattaatagc aatgtcgcca tgcacattta ccttgcagtt
     7261 aattgaataa aaatttaact ggcatcagtc ctaaaaaaat tgatttcatc cgtaggctat
     7321 tgacagaata attcagactg gtctttcagg catccagaca cgctaccgcc cctggctttt
     7381 tagctaccaa tacactgatt gagcttaatt tttcaaaccc tctctgcata caatcgttga
     7441 tgagaaaggg ttattgcgga aattaacttc cgaatataag gtgacattat ggtaattgaa
     7501 tattggctcg ccaataatgc aggaggaagt gttacagcta acggaatagc aggcaaggta
     7561 acaattcggc aattggctat ttttaagaat tatattaagt atcattcaat atggttttta
     7621 ggagtttctt taggttgaca atatttaata ttgcgtctcc acatgcgata tttcttaaat
     7681 aatgttttat tattaccgct tttaattcag ggataatgtg aggttattac cctcaaataa
     7741 tatgagataa atagtgctgc aacattgcat ttttgccccg atttatccat gatcgaattg
     7801 tgacatttgt catacaacga ataggttttg tacttactat ggaatggatt gccgatccgt
     7861 ctatctgggc cgggttaatc acgctgattg tgatcgaact ggtcctcggc attgataacc
     7921 tggtctttat tgccatcctc gccgaaaaac taccgccgaa gcagcgcgac cgcgcacggg
     7981 ttaccgggct gctgctggcg atgttaatgc gcctgttact gctagcgtca atctcctggc
     8041 tggtcaccct gactcaaccg ctgttcagct tccgctcgtt tacctttagc gcccgcgact
     8101 taatcatgct gtttggtggt ttcttcctgt tgttcaaagc cacgatggag ctgaacgaac
     8161 ggctggaagg gaaagacagc aataatccca cacaacgcaa aggtgcgaag ttctggggcg
     8221 tggtgacgca aattgtggta ctggacgcca ttttctcact cgactcagtg attaccgccg
     8281 tcgggatggt cgaccattta ctggtcatga tggccgccgt ggttatcgct atcagcctga
     8341 tgttgatggc cagcaagccg ttaacgcaat ttgttaacag tcacccgacc atcgttattc
     8401 tctgcttaag cttcctgttg atgattggct ttagcctggt ggcagaaggt ttcggcttcg
     8461 tcattccgaa aggctacctg tacgctgcca ttggtttctc ggtgatgatc gaggcactca
     8521 atcagttggc tatctttaac cggcgacgtt ttctttccgc taatcagacg ctacgccagc
     8581 ggactaccga agcggtaatg cgcctgctta gcgggcaaaa agaagatgcg gaactggacg
     8641 ccaaaaccgc gtccatgctg gtggatcatg gaaaccagca gatctttaat ccgcaggaac
     8701 ggcggatgat tgagcgggta cttaatctta accagcgtac cgtaagcagc attatgacgt
     8761 cgcgccacga tattgagcat atcgatctca acgcgccgga agaagaaatc cgccagttgc
     8821 tggagcgaaa tcagcatacg cggctggtgg ttaccgacgg tgatgacgca gaagatttgc
     8881 tcggtgttgt tcacgttatc gacctgctac aacagtcact gcgcggcgaa ccgctcaacc
     8941 tgcgggtgtt gattcgccag ccgctggtgt tcccggaaac cttgccgttg ttaccagccc
     9001 tggagcagtt ccgtaatgcc cgcacgcact ttgcttttgt ggtggatgag tttggctcgg
     9061 tggaagggat tgtgacatta agtgacgtca ctgaaaccat tgccggtaac ttaccgaacg
     9121 aagtggaaga gatcgacgcc cgccatgata ttcagaagaa tgcggacggt tcctggacgg
     9181 cgaatggtca tatgccgctg gaggatctgg tgcaatatgt gccgctgccg ctggatgaaa
     9241 aacgtgaata tcacaccatt gccgggctgt tgatggagta tttgcagcgt attccaaaac
     9301 ctggcgaaga agttcaggtg ggggattatt tgcttaaaac gttgcaggtg gaaagccatc
     9361 gcgtgcagaa ggtgcagatt ataccgctgc gtaaggatgg cgagatggag tacgaggtgt
     9421 gatggtatcg gccattttac ccaatgctgc gtaacgcctt ataacttcag caatgaagcg
     9481 ttgtctgtgg taaacggaca tcacagcggg ggtgtgggtc cggctgtaaa tcgccgaatc
     9541 gtttcctgta ggctggggca gcccacatgg atgtgggctg agggcgcgtt ttacagggat
     9601 gttacctcgc gcccgtaccc gatagccgaa aggaataaga tgagggaacc gcgccagcgg
     9661 cgatttaccg ccgggagccc gggttgccag ggtggtggcg attgagccac cctggcacgt
     9721 tcaccggctt gagcgttcca tagtagcgag gaacatcaag tgaacggaac agccttcgat
     9781 cttacatatt cagaatataa tagtgccgcc gcgaacgcct gatgcgacgc tttacgcgtc
     9841 ttatcaggcc tacaggacag gtcacctggt aacctacatc ttctccagca acttcttcac
     9901 atctttgcca ttgcgggaat ctttattccg ctcggcccag tcattcaggc gacgtttcgc
     9961 ttcgtcctgt agatgtttgc gcagtaactg atccacttgc aggctgtaat tgagttgctg
    10021 ccagttgcca taaacccgca gcggtactgg cgtttctttg aggaaatcaa tcagtttgct
    10081 ttccccgttc cagccaccca cgacccgaat atcaaactgg gtgtcgcagg tttgatctgc
    10141 cagattcaac atgccttccc ctgtcagcgc cagcacaggc gattgacctt gcatgtcgtt
    10201 taacgtcacg acgccatcct tcaacgtcaa atcggtggta aagcggtcaa gccgcgtcac
    10261 gttatcgaag ttttcagcgg ccttcacatc accgccatta cgctctaccg cttgctgaat
    10321 catctgctgg aagttcatcc cttccatacg tgtgtcggtc atctcaacat gcgcctgtcc
    10381 ttgccagttg tggcggaatg cgtcggcatc tatgtcagca ccggagaaat caccagccag
    10441 tgacattttt ccggtcaacg aaatcggata gttaaacgcc ttcagaatgg taccaatctc
    10501 aacgttctcc agccgtggct ggaagtttat ccgcggattt attgatgtcg cgtccagcgt
    10561 gcccggcagt gaaacctttc caccgttaag cttgccctgc agttgagtaa tttccagcaa
    10621 acccgacttg ttggtcattt gcgtggcaac atctgtaaaa ttcattccgc gccagcgcac
    10681 gttactggcc tgcaacaaaa tatcagctgt aaagccttgc agtccctgat aggccggttc
    10741 atcaatacgc gaagaaatga ccgggcgcgg caacgttgat tggctctgcc cctgctgcgc
    10801 ggcaccgttt tcaccattcg cggtttcatt aagcgggatg aggttgtcaa gattcagttg
    10861 cgggaattgc agtctcagct gccattccgg tttctctgtc agcgtgacct gtgcttgtcc
    10921 gctcagcgta ctgtcattgg cggtcaaact aatttggtta aatgaaaggc gtttatgtga
    10981 ctcctgccac tgggactgga aactcccctg cccctgaata ccttgttttg gtaaatcggc
    11041 accctgcaac tgccagttaa tttgttcaat agccgctgtt aaatcatgcg gataatcaga
    11101 agcatctacc gtaccgttaa gggatatcgt gagatcgcgc tgatcgcgat taacccgccc
    11161 ggagaactca aatgagccac gatgttgggg atcttgttcc atttgcaggc ggatattgcg
    11221 gattgtcacc tgctcgtcat cttcatgctg gaacaccaga acgctgtccg ccaccttaag
    11281 actggatata tcaaacgacc atccgcgatc gtctgacaga tccggcaagg tattgtcgcg
    11341 cggtgcaacc ggagcgtctt cactgcgcac cgcttccgtc tgcggcgtca gttggatcac
    11401 tgcccctttt agcatcacct gcttaacgct cagttgatga ctcagtagtg gtaaaagcgc
    11461 cacgtccaga cgcatgttgt cggcgcgaac cagtggctgg cttgcgccct gggcggtgag
    11521 agacattcgc ccggagagga tactaagctg cggccagacg tgccaacgca gtggcccgtc
    11581 gagctgcaat tgataaccgc tacgtgcagc aacttgcttg accatatagt cgcggaaatc
    11641 attcggattc accagcaaca ctaacgcaga taacccggcc accagcacga ccaagagtat
    11701 catcagcgtc gtcagaaatc gtctcatggt atcctcaatg ggcctgaatt agtctttatc
    11761 gattcggctg gctaccgcgc cctgctggtt gcgatatttc gcatcttcac ggcggttgta
    11821 aggtcgcgcc gccgggccgg aaagcggctc aaagctcagc gcaccaatta acatgcccgg
    11881 acgcagcgcc agcggcagct taccggagtt gtagaactcc agcacaatgc aaccagacca
    11941 gcccggatcg atacggtgcg cggtgacgtg caccatcagc cccagacgcg ccaaagagga
    12001 acgcccgtcc agccagccca ccagatcggc tggcagcgtc accgactcca gtgtcaccgc
    12061 cagcgccagc tctcctgggt gaagatagaa cgcctcgccc tcgtcgagaa cgatctcatc
    12121 gctcatcacg cggtcaagcg cggcgctcac ttcatctttg ggaccgctca gatcgataaa
    12181 cgctgccgtg tgaccacgga aggtacgaaa tttattgccc aggcgtacat ccaccgtcgc
    12241 gccgttaata cgctccactg gcggacgtgg gttgatcgac aaacggcctt catcaagcca
    12301 ggcttcaata tctcggtcac acagacgcat ggcattttct cctttcgcac atcactccct
    12361 taacgccaat tacgtcaagg gcataccaac ttatcactga acggtacaca atttatcaag
    12421 cttattcaaa gaactgactt attttcgctt tcaatatatc gatcgcgatg cggtttttcc
    12481 cgccgcgcgg cacgataatg tccgcatatt gtttagaagg ctcaatgaat tgcaggaaca
    12541 tcgggcgcac ggttttttga tattgcgcca tcacggaatc cattgaacgc ccacgctcgt
    12601 taacgtcacg cttgatgcgg cgcatcaggc agatatccag cggggtatca acgaaaatgg
    12661 agaagttaag ttcgtcacgc aaacgcgcat ccgtcagcaa caaaatgcct tcgagaatga
    12721 tgaccttctt cggctcaacc gtcaccgttt ctttcatacg cgtatgttca acatagctgt
    12781 aaaccggcag gtcaattgcc gagccgcgtt tcaacgcttg taaatgctca agcaacaggc
    12841 tgtgatccat cgcgctggga tggtcgtagt tggtcttaac gcgttcttcc atcgacagat
    12901 ggctttgatc tttgtaatag cagtcttcgg gaattacgcc gatgtgttca tcaccgactt
    12961 gctcacgtaa ttcacgataa agggtactgg caataagact cttgccggaa gccgatgcgc
    13021 cagcgatacc gataatgacg cactgatgag actgatcagt catatattta gcgacctgat
    13081 taacctggat gttaggaagg gggcgacgaa gcgccaaacg cggcaattat agggatttca
    13141 tccgtctgat accagtcgaa tagcgttgcc gcgcgctcag agttaattgt tgacaaagaa
    13201 ttcccggtgg caaattacgt tgatcagttt tatgtaaggt aaaaaatgtt atacgcagtt
    13261 gcgcaaatta tccgccttta cgtcacttta tgagcaattc gcatataaaa tgtaaaactt
    13321 ttgtactagc ataaacacag aaacgaatac tggcgacctg gccttgcgga taaagcggta
    13381 ataagcaaac aatcacagca tgtattaatt gccctgcccc acccgctgct tcacctggtc
    13441 agtttaggtt tagtctcgtt tatctttacc cttgtctcgc ttgagctttc gcagtttggc
    13501 acccaactcg ccccactgtg gttcccgacg tccatcatga tggtggcgtt ttatcgccat
    13561 gccgggcgca tgtggccggg aattgcgctg agctgttcgc tgggaaatat cgccgcatcc
    13621 attccaggaa ggccagcagg tcaaagcagg cgatttactg gcagaaattg accccagcca
    13681 gttcaaagtt gcattagcac aaacccaggg ccaactggca aaagataaag ccacgcttgc
    13741 taacgcccgc cgtgatctgg cgcgttatca gcaactggca aaaaccaatc tcgtctcccg
    13801 tcaggagctg gatgcccaac aggcgctggt cagtgaaacc gaaggcacca ttaaggctga
    13861 tgaagcaagc gtcgccagcg cgcagttgca actcgactgg agccgcatca ccgcaccagt
    13921 cgatggtcgc gttggtctca agcaagttga tgttggtaac caaatctcca gtggtgatac
    13981 caccggaatt gtggtgatca cccagacgca tcctatcgat ttagtcttta ccctgccgga
    14041 aagcgatatc gctactgtag tgcaggcgca aaaagccgga aaaccgctga tggtagaagc
    14101 ctgggatcgc accaattcga agaaattaag tgaaggcacg ctgttaagcc tcgataacca
    14161 aatcgatgcc acaaccggta cgattaaagt gaaagcacgc tttaataatc aggatgatgc
    14221 gctgtttccc aatcagtttg ttaacgcgcg catgttagtc gacaccgaac aaaacgccgt
    14281 agtgatccca acagccgccc tgcaaatggg caatgaaggc cattttgtct gggtgctgaa
    14341 tagcgaaaac aaggtcagca aacatctggt gacgccgggc attcaggaca gtcagaaagt
    14401 ggtgatccgt gcaggtattt ctgcgggcga tcgcgtggtg acagacggca ttgatcgcct
    14461 gaccgaaggg gcgaaagtgg aagtggtgga agcccagagc gccaccactc cggaagagaa
    14521 agccaccagc cgcgaatacg cgaaaaaagg agcacgctcc tgatgcaggt gttacccccg
    14581 agcagcacag gcggcccgtc gcgcctgttt attatgcgtc ctgtcgccac cacgctgctg
    14641 atggtggcga tcttactcgc cgggattatc ggttatcgcg ccctgcccgt ttcggcgctg
    14701 ccggaagtgg actatccgac cattcaggtg gtcacgctct acccaggtgc cagcccggat
    14761 gtcatgacct ctgccgttac cgcgccgctc gaacgccagt tcgggcagat gtctggcctg
    14821 aaacagatgt cgtcgcaaag ttccggcggt gcgtcagtta tcactttgca gttccagcta
    14881 acattaccgc tcaatgtcgc cgagcaggaa gtgcaggccg cgattaacgc tgcgaccaac
    14941 ttgttgccga gcgatctgcc taacccgccg gtttacagca aagtgaaccc ggcagatccg
    15001 ccgatcatga cgctcgccgt cacctcaacc gccatgccga tgacgcaagt ggaagatatg
    15061 gtggaaaccc gcgtcgcgca gaaaatctcg cagatttccg gcgtcggcct ggtgacgctt
    15121 tccggcggtc agcgtccggc tgttcgcgtc aaacttaacg ctcaggcgat tgccgccctc
    15181 ggcctgacca gcgaaaccgt gcgcaccgtc attaccggcg ctaacgttaa ctcggcaaaa
    15241 ggtagcctcg acggcccttc ccgtgcggtc acgctttccg cgaacgacca gatgcaatcc
    15301 gccgaagagt atcgccagct aatcatcgcc taccagaacg gcgcgccaat tcgtctgggc
    15361 gatgtcgcaa ctgtagagca aggtgcagaa aacagctggc tcggcgcgtg ggcgaacaaa
    15421 gaacaggcca ttgtgatgaa tgttcagcgc cagcccggtg ctaacattat ctccaccgcc
    15481 gacagcattc ggcagatgct gccacagctc actgagagtc tgccgaaatc ggtgaaggtg
    15541 acagtgcttt ccgatcgcac caccaatatc cgcgcatccg tcgatgatac tcagtttgaa
    15601 ttgatgatgg ctatcgcgct ggtagtcatg attatctacc tgtttttgcg caatattccg
    15661 gcgaccatca ttcccggtgt tgctgtaccg ctgtcgttaa tcggcacttt cgcggttatg
    15721 gtgtttctcg atttttcaat caataacctg acactgatgg ctttaactat cgccaccgga
    15781 ttcgtggtcg atgacgccat cgtggtgatc gaaaacattt cccgctatat cgaaaaaggc
    15841 gaaaaaccgt tggcggcggc gctcaagggc gcaggtgaaa tcggctttac cattatctcg
    15901 ctgaccttct cactgattgc ggtgttgatc ccactgctgt ttatgggcga tatcgtcggg
    15961 cgactgttcc gcgaatttgc tattaccctg gcggtagcga ttttgatctc agcggtggtg
    16021 tcgctgaccc tgacaccgat gatgtgcgcg cggatgctca gccaggagtc gttgcgtaaa
    16081 cagaacagct tctcccgtgc ctcggaaaaa atgttcgaca ggataatcgc cgcctatggt
    16141 cgtggactgg cgaaagtgct gaatcatccg tggctgacct taagcgtggc actcagcacg
    16201 ctgctgctta gcgtgctgct gtgggtgttc attccgaaag gtttcttccc ggtacaggac
    16261 aacggcatta ttcagggcac tttgcaggca ccgcaatcca gctcctttgc caatatggcc
    16321 cagcgacaac gccaggtcgc ggacgtgatt ttgcaggatc cggcagtgca aagcctgacc
    16381 tcatttgttg gcgttgatgg cactaacccg tcgctgaata gtgcacgttt acaaatcaac
    16441 ctcaaaccgt tggatgaacg tgatgatcgg gtgcaaaaag tcatcgcccg tctgcaaacg
    16501 gcggtagata aagtgccggg cgtcgatctc ttcctgcaac caacgcagga cctgaccatc
    16561 gatactcagg tcagccgcac ccagtaccag tttaccttgc aggccacgtc actggatgcg
    16621 ctcagtacct gggtgccaca gttgatggaa aaactccagc aactgccaca gctttctgat
    16681 gtctccagcg actggcagga caaagggctg gtggcgtatg tcaatgttga tcgcgacagc
    16741 gccagccgtc tggggatcag catggcggac gtcgataacg ccctgtacaa cgcgtttggt
    16801 cagcgactga tttccactat ttatactcag gctaaccagt atcgcgtggt gctggagcac
    16861 aataccgaaa ataccccagg cctcgcggcg ctggatacca ttcgcctgac cagcagcgac
    16921 ggtggcgtgg tgccgttaag ctcaattgcc aaaattgagc agcgttttgc gccgctttcc
    16981 atcaaccatc tggatcagtt cccggtaaca accatctcct ttaacgtgcc ggataactat
    17041 tcgctgggcg atgcggtgca ggcgattatg gacaccgaaa agacgctgaa tctgccggtg
    17101 gatatcacca cgcaattcta gggcagcacc ctcgccttcc agtcggcgct gggcagcact
    17161 gtctggctga ttgtcgcggc ggtggtggcg atgtatatcg tgctcggcat tctgtacgag
    17221 agctttattc acccgatcac cattctctcg acgctaccca ccgcaggggt cggcgcgctg
    17281 ctggcattaa tgattgccgg tagcgaactg gatgtgattg cgattatcgg cattattttg
    17341 ctgatcggta ttgtgaagaa gaacgccatc atgatgatcg acttcgcgct ggctgctgag
    17401 cgcgagcaag gcatgtcgcc gcgcgatgca atctaccagg cttgtctgtt gcgttttcgt
    17461 ccgatcctga tgaccaccct ggcggctctg cttggcgcgc tgccgctgat gttgagtacc
    17521 ggggtcggcg cggaactgcg tcgtccgtta ggtatcggca tggtcggcgg tctgattgtc
    17581 agccaggtgc tgacgctgtt caccacgccg gtgatttatc tgctgttcga ccgcctggca
    17641 ttgtggacca aaagtcgctt tgctcgccat gaagaggagg cgtaagtgaa gttttttgcc
    17701 ctcttcattt accgcccggt ggcgacgatt ttactgtcgg tcgccattac cctgtgcggc
    17761 atactgggtt tccgtatgct gccggtcgcc ccgctgccgc aggtcgattt tccggtgatt
    17821 atggtcagcg cctcgctgcc cggtgcgtcg ccggagacaa tggcgtcttc cgttgccacg
    17881 ccgctggagc gctcacttgg gcgcattgcc ggggttagtg aaatgacctc cagcagttcg
    17941 ctcggcagca cgcgcattat tttgcagttt gattttgacc gggatatcaa cggcgcagcg
    18001 cgtgatgtgc aggcggcgat caacgctgca caaagtttgc tgcccagcgg gatgcccagc
    18061 cgcccgacct atcgcaaagc gaacccgtcg gatgcgccaa ttatgatcct cacgctgaca
    18121 tccgatactt attcgcaggg tgaactgtac gatttcgcct cgacgcagct ggctccgacg
    18181 atttcgcaaa tcgacggtgt cggtgatgtc gatgtcggtg gcagctcgct gcccgccgtg
    18241 cgcgtcgggc tgaccccgca ggcgctgttt aatcagggcg tgtcgctgga cgacgtacgc
    18301 actgccatca gcaatgccaa cgtgcgtaaa ccgcaggggg cgctggaaga tggcactcac
    18361 cgctggcaga tccagaccaa cgatgaactg aaaaccgccg ctgaatatca gccgctgatt
    18421 attcactaca acaacggcgg cgcggttcgt ctgggcgatg tggcgacggt gactgactca
    18481 gtgcaggatg tgcgcaacgc cgggatgacc aacgccaaac cggctatttt actgatgatc
    18541 cgcaaactgc cggaagccaa tattatccag acggtagaca gcatccgggc aaaattacca
    18601 gagttgcagg agaccattcc ggcggcgatt gatctgcaaa ttgcccagga tcgctccccc
    18661 accattcgcg cctcgctgga agaagtcgag caaacgctga ttatctcggt ggcgctggtg
    18721 attctggtgg tctttttatt cctgcgctcg ggtcgcgcca ctattattcc cgccgttgcg
    18781 gtgccggttt cgctgattgg tacgtttgcg gcgatgtacc tgtgcggatt cagtctcaat
    18841 aatctttcgc tgatggcgct caccattgct actggtttcg tggtggatga cgccattgtg
    18901 gtgctggaaa acattgcccg tcatctggaa gcggggatga aaccactaca ggccgcgctg
    18961 caaggtactc gcgaagtcgg ctttacggtg ctgtcgatga gtctgtcact ggtggcggtg
    19021 ttcctgccgc tgctgttgat gggcggattg ccgggccgac tgttacgcga gtttgccgtg
    19081 acgctttctg tcgccattgg tatttcgttg ctggtttccc tgacgctaac gccaatgatg
    19141 tgcggctgga tgctaaaagc cagcaagccg cgtgagcaaa agcgactgcg tggttttggc
    19201 cgcatgttgg tagccctgca acaaggctac ggcaagtcgc tgaaatgggt gctcaatcat
    19261 acccgtctgg tgggcgtggt gctgcttggc accattgcgc tgaatatctc gatcccgaaa
    19321 accttcttcc cggagcagga caccggcgta ctgatgggtg ggatacaggc ggatcagagt
    19381 atttcgtttc aggcgatgcg cggcaaattg caggatttca tgaaaattat ccgtgacgat
    19441 ccggcagtgg ataatgtcac cggctttacc ggcggttcac gagttaacag tgggatgatg
    19501 tttatcaccc tcaagccacg cgacgaacgc agcgaaacgg cgcagcaaat tatcgaccgt
    19561 ctgcgcgtga agctggcgaa agaaccgggg gcgaatctgt tcctgatggc ggtacaagat
    19621 attcgcgttg gtgggcgtca gtcgaacgcc agctaccagt acacgttgtt atccgacgac
    19681 ctggcggcac tgcgcgaatg ggagccgaaa atccgcaaaa aactggcgac gttgccggaa
    19741 ctggcggacg tgaactccga tcagcaggat aacggcgcgg agatgaatct ggtttacgac
    19801 cgcgacacca tggcacggct gggaatcgac gtacaggccg ccaacagtct gttaaataac
    19861 gccttcggtc agcggcaaat ctcgaccatt taccagccga tgaaccagta taaagtggtg
    19921 atggaagtgg atccgcgcta tacccaggac atcagtgcgc tggaaaaaat gttcgttatc
    19981 aataacgaag gcaaagcgat cccgctgtcg tatttcgcta aatggcaacc ggcgaatgcc
    20041 ccactatcgg tgaatcatca gggattatcg gcggcctcga ccatctcgtt caacctgccg
    20101 accggaaaat cgctctcgga cgccagtgcg gcgatcgatc gcgcaatgac ccagcttggt
    20161 gtgccttcga cggtgcgcgg cagttttgcc ggcacggcgc aggttttcca ggagacgatg
    20221 aactcgcagg tgatcctgat tatcgccgcc atcgccacgg tgtatatcgt gctgggtatc
    20281 ctttacgaga gttacgtaca tccgctgacg attctctcca ccctgccctc ggcgggcgtt
    20341 ggagcgctgt tggcgctgga gctgttcaat gccccgttca gcctaatcgc cctgataggg
    20401 atcatgctat taatcggcat cgtgaagaaa aacgccatta tgatggtcga ttttgcgctt
    20461 gaagcccaac ggcacggtaa cctgacgccg caggaagcta ttttccaggc ctgtctgctg
    20521 cgttttcgcc cgattatgat gaccaccctg gcggcgctgt ttggcgcgct gccgctggta
    20581 ttgtcgggcg gtgacggctc ggagctgcgg caacccctgg agatcaccat tgtcggcggt
    20641 ctggtgatga gccagctcct gacgctgtat accacgccgg tggtgtatct ctttttcgac
    20701 cgtctgcggc tgcgtttttc gcgtaaacct aaacaaacgg taaccgagta aatgacagat
    20761 cttcccgaca gcacccgctg gcaattatgg attgtggctt tcggcttctt tatgcagtcg
    20821 ctggacacca ccatcgtaaa caccgccctt ccctcaatgg cgcaaagcct cggggaaagt
    20881 ccgttgcata tgcacatggt cattgtctct tatgtgctga ccgtggcggt gatgctgccc
    20941 gccagcggct ggctggcgga caaagtcggc gtgcgcaata ttttctttac cgccatcgtg
    21001 ctgtttactc tcggttcact gttttgcgcg ctttccggca cgctgaacga actgttgctg
    21061 gcacgcgcgt tacagggcgt tggcggtgcg atgatggtgc cggtcggcag gttgacggtg
    21121 atgaaaatcg taccgcgcga gcaatatatg gcggcgatga cctttgtcac gttacccggt
    21181 caggtcggtc cgctgctcgg tccggcgctc ggcggtctgc tggtagaata cgcgtcgtgg
    21241 cactggatct ttttgatcaa cattccggtg gggattatcg gtgcgatcgc cacattgatg
    21301 ttaatgccga actacaccat gcagacgcgg cgctttgatc tttccggctt tttattgctg
    21361 gcggttggca tggcggtatt aaccctggcg ctggacggca gtaaaggtac aggtttatcg
    21421 ccgctggcga ttgctggcct ggtcgcagtt ggcgtggtgg cactggtgct ttatctgctg
    21481 cacgccagaa ataacaaccg cgccctgttc agtctgaaac tgttccgcac tcgtaccttt
    21541 tcgctgggcc tggcggggag ctttgccgga cgtattggca gtggcatgtt gccctttatg
    21601 acaccggttt tcctgcaaat tggcctcggt ttctcgccgt ttcatgccgg actgatgatg
    21661 atcccgatgg tgcttggcag catgggaatg aagcgaattg tggtacaggt ggtgaatcgc
    21721 tttggttatc gtcgggtact ggtagcgacc acgctgggtc tgtcgctggt caccctgttg
    21781 tttatgacta ccgctctgtt gggctggtac tacgttttgc cgttcgtcct gtttttacaa
    21841 gggatggtca actcgacccg tttctcctcc atgaacaccc tgacgctgaa agatctcccg
    21901 gacaatctgg cgagcagcgg aaacagcctg ctgtcgatga ttatgcaatt gtcgatgagt
    21961 atcggcgtca ccatcgccgg gctgttgctg gggctttttg gttcgcagca tatcagcgtc
    22021 gacagcggca ccacacaaac cgtctttatg tacacctggc ttagcatggc gtttatcatc
    22081 gcccttccgg cgttcatctt tgccagagtg ccgaacgata cgcatcaaaa tgtagctatt
    22141 tcgcggcgaa aaaggagcgc gcaatgaagt tctggcgacc cggtattacc ggcaaactgt
    22201 ttctggcgat tttcgccacc tgcattgtct tgctgatcag tatgcactgg gcggtgcgta
    22261 tcagttttga gcgtggcttt attgattaca tcaaacacgg caatgaacag cgattacagc
    22321 tgttaagtga tgcgcttggc gagcagtatg cgcagcatgg taactggcgc ttcctgcgca
    22381 acaacgatcg ctttgtcttt cagatcctgc gttcatttga acacgataat tcggaagata
    22441 aacccggccc gggtatgccg ccgcacggct ggcgtaccca gttctgggtg gtcgatcaaa
    22501 acaacaaagt actggttggt ccgcgagcgc cgattccacc tgacggtaca cggcgaccca
    22561 ttctggtcaa cggtgcggaa gtgggcgcgg tgattgcctc ccctgttgag cggctgaccc
    22621 gcaatactga tatcaatttc gataaacaac agcggcaaac cagctggctg atcgtcgccc
    22681 tggcaacgtt actcgcggcg ctcgccacct ttctgctggc gcgcggtttg ctggcaccgg
    22741 taaaacgact tgtcgatggc acgcacaaac tggcggcggg cgatttcact acccgcgtaa
    22801 cgcccaccag tgaagatgaa ctgggcaaac tggcgcaaga cttcaaccag ctcgccagca
    22861 cgctggagaa aaaccagcaa atgcggcgcg attttatggc cgatatttct cacgaactgc
    22921 gtacgccatt agcggtgctg cgcggtgaac tggaagccat tcaggatggc gtgcgcaaat
    22981 tcacgccgga gacggtggcg tctttacagg cggaggtcgg tacactgacc aaactggttg
    23041 acgatcttca tcagttgtcg atgtctgatg aaggcgctct cgcctatcaa aaagcaccgg
    23101 tagatttgat cccactgctg gaagtggcgg gcggcgcatt tcgcgaacga ttcgccagcc
    23161 gtggcctgaa actgcaattt tccctgccag acagtatcac cgtatttggc gatcgcgacc
    23221 gtttaatgca gttattcaat aacttactgg aaaacagcct gcgctacact gacagcggcg
    23281 gcagcctgaa aatctctgcc gagcagcacg acaaaacggt gcgcctgacc tttgccgaca
    23341 gtgcgccagg tgtcagtgac gatcagctac aaaaattgtt tgaacgtttt tatcgcaccg
    23401 aaggttcccg caaccgcgcc agcggcggtt ccgggctggg gctggcgatt tgcctgaaca
    23461 ttgttgaagc ccataatggc cgcattattg ccgcccattc gccttttggc ggggtaagca
    23521 ttacagtaga gttaccgctg gaacgggatt tacagagaga agtatgaccg agttaccaat
    23581 cgacgaaaac acaccgcgta ttttgatcgt ggaagatgaa ccgaagctgg ggcagttgct
    23641 cattgattat ctgcgtgctg cgagctatgc gccgacgctt atcagccacg gcgatcaggt
    23701 actggcgtat gtgcgccaga caccaccgga tctgatcctg ttggatctga tgctccctgg
    23761 caccgatggc ctgacattat gccgggaaat tcgtcgtttt tccgatgttc cgatcgtgat
    23821 ggtgacggca aaaatcgaag agatcgaccg cctgctgggg ctggagattg gcgccgatga
    23881 ctacatctgc aagccgtaca gcccacggga agtggtagcg cgtgtcaaaa ccattttgcg
    23941 ccgttgtaaa ccgcaacgcg aacttcagca acaggatgcc gaaagcccgt tgattatcga
    24001 cgaaggtcgt tttcaggctt catggcgcgg taaaatgctt gacctgacgc ctgcggaatt
    24061 tcgtctgctt aaaacgctct ctcacgaacc aggaaaagtg ttctcccgcg agcaattgct
    24121 caatcatctt tatgacgact accgcgtagt aaccgaccgc accattgaca gccacattaa
    24181 aaatctgcgc cgcaagctgg aatctctcga cgccgaacag tcatttatcc gcgccgttta
    24241 cggcgttggt taccgctggg aagccgacgc ctgccgcatc gtttagtttt agcgacatta
    24301 ctttgttagc cggagatggc gttcagaccc gatccggcaa cattattcca cgcatggtca
    24361 gcaaactgac attatctctc cgccctggca ggcatcagaa aatcgctcat actttaatca
    24421 gtaaacagca cctttagatg ctgttttcga taagcaattt caatcaagga gtcattatgg
    24481 ctggttggtt tgaactcagt aagagcagtg ataatcagtt ccggtttgtg ctaaaagcgg
    24541 gcaatggtga gactatcctc accagcgagc tgtatacctc aaaagcctct gcggaaaagg
    24601 gcatcgcgtc ggtgcgtagc aacagcccgc aagaagagcg ctatgagaaa aaaacggcaa
    24661 gcaacggcaa attctatttc aatctgaaag ccgctaatca tcaaattatc ggctccagcc
    24721 agatgtacgc caccgcgcaa tctcgtgaaa ccggaattgc ctccgttaaa gccaatggca
    24781 caagccagac ggtgaaagac aatacgtaat tacgatgccg ggcgtgggtt gttggcgtcc
    24841 ggcgctttac cagatccccc ctacagcgct acaatgcccg cccttaaagt gggggcctcc
    24901 cctaaccgct tcatcaggtg aagcggatct gacctgtcat cagaacgaga gaattatgtt
    24961 taaaccggaa ctcctttccc cggcgggaac gctgaaaaat atgcgttacg ctttcgctta
    25021 tggcgcagat gctgtttatg cgggccagcc gcgttactcc ctacgtgtgc gcaacaacga
    25081 attcaaccac gaaaatcttc agctcggcat caatgaagcc cacgcgctgg ggaaaaagtt
    25141 ttatgtcgtg gtcaacattg caccgcacaa cgccaagctg aaaaccttta tccgtgacct
    25201 gaaaccggtg gtggaaatgg ggccggatgc gctgattatg tccgatccag ggctgattat
    25261 gctggtgcgt gagcacttcc ctgaaatgcc gatccacctc tcggtgcagg ctaacgccgt
    25321 gaactgggcg acggtgaaat tctggcagca aatgggactg acccgcgtga tcctctctcg
    25381 cgagctgtcg ctggaagaga ttgaagagat ccataatcag gtgccggata tggagatcga
    25441 gatcttcgtt cacggcgcac tgtgcatggc ctactccggt cgctgcctgc tctctggcta
    25501 tatcaacaag cgcgacccga accagggcac ctgcaccaac gcctgccgct gggagtacaa
    25561 cgtccaggaa gggaaagaag atgacgttgg caacatcgta cacaagtacg agccgattcc
    25621 ggtgcaaaat gttgagccga cgctgggtat cggcgcgcca accgacaaag tgtttatgat
    25681 cgaagaagcc cagcgtccgg gcgagtatat gaccgcgttt gaagatgagc acggcactta
    25741 catcatgaac tcgaaagatc tgcgcgccat cgcccatgta gaacgcctga ccaaaatggg
    25801 cgtgcattcg ctgaaaatcg aaggtcgtac caaatctttc tactattgcg cacgcaccgc
    25861 acaggtttac cgcaaagcta tcgatgacgc cgctgcggga aaaccgttcg ataccagcct
    25921 gctggaaact ctggaaggtc tggcgcatcg tggctatacc gaaggtttcc tgcgtcgtca
    25981 tactcacgac gattatcaga actacgaata cggttattca gtttctgacc gccagcagtt
    26041 tgttggtgag tttaccggtg agcgcaaggg ggacctcgca gcggtagcgg tgaaaaataa
    26101 attctccgtt ggcgacagtc ttgagctgat gacgccgcaa ggcaacatta actttaccct
    26161 tgagcacatg gaaaacgcca aaggcgaagc tatgccggtc gcaccaggcg atggttatac
    26221 tgtgtggctc ccggtcccgc aggatcttga gctaaattac gcgctgctaa tgcgtaattt
    26281 ctccggggaa accacgcgta acccccacgg taagtgatta atttcgatta tttttcccgg
    26341 atggaaaatt cttagaaacc gatcacatac agctgcattt attaaggtta tcatccgttt
    26401 cgctgaaaaa cataatccat aaaatgctag ctgtaccagg aaccacctcc ttagcctgtg
    26461 taatctccct tacacgggct tattttttgt acataagctg taaagaagga ttgatccctg
    26521 ataaccgtct tccattgacc acatcgatag aatccccctt catagcacaa tgcctttcac
    26581 gtaacggtat cgtgctcgcc caggttccgg ctacgcacag ccagaacgcg catgtttgac
    26641 gcttacaaaa aaatattctc actctccaca tttgaatgtc agacgagcga cgccatgtaa
    26701 tcctgcacct tctgtcttca ggtcaactat ctgcattttt ttgccctgag taacacagaa
    26761 atgggctgca tcatttttta ctatattttc tgcaccagat attctgcccc tggctaaaga
    26821 agcttcggct tcggtgtagt attggttatc gagtttacgc tgaatattac ttttatatgc
    26881 aagaccaaat ttaccgatac ttgtttcatc attatgcaca gcacaaccag atataagaaa
    26941 aatactaatt aatgatatag cagctatctt tttcatctca ccttccccca ttaaatacca
    27001 acgacactct ctagtgttta aatataataa tggcatgatt attataattg aataggatta
    27061 taataaatgt tctgtacaac atttcctaca taagtaggaa ttacggacat tgaggccctt
    27121 cagggtaact ccatgggggc tttaatatat tatattgaag atgccactgt ttagttgaat
    27181 attaggtata tgctcttttt tgaaatttat cggtggcagc cgttagtatt cgctgtcccc
    27241 attgcaagct cctggtggta accactgaat cctccatact tgaactgact ttttatcctc
    27301 cgactttcat cctgttctga ctccaccttt tgttttctgc tctacactat ctacagacca
    27361 atcataaagg cacatacgat catggcagaa tttcccgcca gcttactgat tcttaatggt
    27421 aaaagtactg acaatctacc cttgcgcgaa gcaattatgc tgttgcgtga ggaaggaatg
    27481 acgatccatg tgcgggtcac ctgggagaaa ggtgatgccg cacgatttgt agaggaggcc
    27541 cggaagttgg gcgtcgcaac ggtgattgcc ggtggtggcg atggcaccat taatgaagtt
    27601 tctacggcgt tgattcagtg tgagggggat gacatacccg cgctgggaat tttgccatta
    27661 ggaaccgcca atgattttgc caccagtgta gggattcctg aggcactgga taaggcgctg
    27721 aaactggcaa ttgccggtaa tgccattgcg atagatatgg cgcaggtcaa caaacaaacc
    27781 tgttttatta atatggcgac aggcggattt gggacgcgta ttaccacaga aacgccggaa
    27841 aaattaaaag ccgcgctggg tggcgtctct tacatcattc atggcttaat gcgcatggat
    27901 actctgcaac cggaccgttg tgaaatccgc ggtgaaaact ttcactggca aggtgacgcc
    27961 ctggtcattg gtattggtaa cgggcgtcag gccggtggcg gtcaacaatt gtgcccgaac
    28021 gcgttaatta acgatggctt gctgcaactg cgcattttta ccggcgatga aattcttccg
    28081 gctctcgtat caaccttaaa atctgacgaa gataacccga atattatcga aggcgcttcg
    28141 tcgtggtttg atatacaagc cccacacgaa atcactttta atcttgatgg cgaaccgttg
    28201 agtgggcaaa acttccatat tgaaatactt ccggcggcgt tgcgttgtcg attaccacca
    28261 gattgtccat tattgcgtta atcaaactat tctctcttga accagggaga atccgtaatg
    28321 ccggatgcgg tgtaaacacc ttatccggca tacagaacaa tactacaccg taattaattt
    28381 tacccgtgtc ttctgcaatg ccaatttatc cacctctgac acagcatcat cggtaacaat
    28441 aatgtcaatt ttttcgattg gcagtacctg attaaaaccg cgacgattaa atttcgatga
    28501 atcgagaacg gcaatcactt tatttgcggc ggtgaccatc gccccgctaa tcgaataacc
    28561 ttcattaaag gtcgtaatac cattaaccgc atcaatacca tcagcaccga caaacattaa
    28621 atcggcatta atatcctgca atgaacgctc ggcaatggaa ccatgcatcg agcgcgtttt
    28681 atggcggacc gtgccaccac agacgaccag agtaatgtct ttattttcgg aaagggcaaa
    28741 cgctgccggg agactgttgg taatcaccgt gatattttta gcggtcatta atccttcggc
    28801 gataagcatt gtggtacttc cgctgtcgag aataacggtc atcccttcat ggatcatttt
    28861 gactgccgcc tgcgctattc tgttttttgg cgcgctggcg agctgaaatc gctctttaaa
    28921 cccgacttcc tgggtctcgg tttcactatt accggacatt attttcgccg caccgccatg
    28981 aaagcgcgta accacgcctt tttgttcgag aaagcgcaaa tcggcacgga ttgtcgcttc
    29041 cgaggcagca aatactcccg ccagatcctg aacaagcacg gttccctgtt cattcactaa
    29101 ttggatgatc ttattccttc gctcgaatga attcatcgtc atttacccat attcaattgt
    29161 ggctagtgta aacgaagggg gaccattggt gaatgatctg ttttgcacaa taccgggcca
    29221 gcatcacgct ggcccgcggt ttcatggaat gagcaacact ttgccctgca tggcattacg
    29281 agcgatgtca cgcaccgcct gggtgaagct ttcaaagctt ccacggtgag cgattaatgg
    29341 ctccaggctt aacttacgtt ctgtcagcaa ccggctcgcc gtttcccact cctgccccgg
    29401 ccaagggctg gaatagttca tccaactgcc gataaccgtc agctctttac gcaatatttt
    29461 gccaaacgtt gccgatgtta aatgcagatc ctgatgcaac gtgcccacca gcgccagttg
    29521 ggcatgagga ccggcaatct ctaccgccag ttcgacagtt tgcggtacgc cagccgtctc
    29581 gaggataagc tgattaaagc gcagttcgcg taaaacgctc tgcatttgcg gcgcgctcat
    29641 ttcgctactg ttaaatgttt gcatcgcacc gaaagatttt gccagtgcca gtttttctga
    29701 gctaatgtcg atcgccgtca cactctttgc tcccagcgcg acagcgcact gaatagccag
    29761 caggccaatg gttccggcac caataataat aacgttttta ttctcacaac cttgcgctaa
    29821 atgaaaagca tgcaggccaa cggtaatcgg ctcaataaaa gccccatcct caataggcat
    29881 atccgtgggt agagcaaaga cattttttcg cttaacgaca atatattcag caaatccacc
    29941 atcacgccgc gagccaataa aatcatattt tgcgcactgg gaataaaacc ctttcaaaca
    30001 ctctggacaa gtaaaacagg gtaataacgg cacacaggca accgcatcgc cagggtgtaa
    30061 atcatcaaca ccggatccca ccgcatcaat atagccgcta aattcatggc ctaacgttat
    30121 tggataataa tgtgcaccat ttttaaatat cctgggtaaa tcggaaccac ataagcccga
    30181 gctggcaatt tttacccgca cctcatcctg atgtttaatt tcaggaatga cgctttttgc
    30241 aacgcgcacg ataccatcag tatcattcac tactgatttc ataaaaactc ctgat
//
LOCUS       DAJTSI010000035        30012 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000035 DAJTSI010000000
VERSION     DAJTSI010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30012
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.035"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            248..631
                     /gene="secE"
                     /locus_tag="OO327_001404"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="OO327_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR6018003.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="OO327_001405"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="OO327_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR6018004.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="OO327_001406"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="OO327_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR6018005.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="OO327_001407"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="OO327_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR6018006.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="OO327_001408"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="OO327_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR6018007.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="OO327_001409"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="OO327_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR6018008.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="OO327_001410"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="OO327_001410"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR6018009.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLD
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="OO327_001411"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="OO327_001411"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR6018010.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO327_001412"
     CDS             complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO327_001412"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR6018011.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO327_001413"
     CDS             complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO327_001413"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR6018012.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO327_001414"
     CDS             complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO327_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR6018013.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO327_001415"
     CDS             complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO327_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCR6018014.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO327_001416"
     CDS             complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO327_001416"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR6018015.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO327_001417"
     CDS             complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO327_001417"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR6018016.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18150..18250)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO327_001418"
     CDS             complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO327_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR6018017.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18874..19647
                     /gene="nudC"
                     /locus_tag="OO327_001419"
     CDS             18874..19647
                     /gene="nudC"
                     /locus_tag="OO327_001419"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR6018018.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19687..20751
                     /gene="hemE"
                     /locus_tag="OO327_001420"
     CDS             19687..20751
                     /gene="hemE"
                     /locus_tag="OO327_001420"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR6018019.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20908..21579
                     /gene="nfi"
                     /locus_tag="OO327_001421"
     CDS             20908..21579
                     /gene="nfi"
                     /locus_tag="OO327_001421"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR6018020.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21622..22212
                     /locus_tag="OO327_001422"
     CDS             21622..22212
                     /locus_tag="OO327_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR6018021.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22399..22671
                     /gene="hupA"
                     /locus_tag="OO327_001423"
     CDS             22399..22671
                     /gene="hupA"
                     /locus_tag="OO327_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR6018022.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22684..23379
                     /locus_tag="OO327_001424"
     CDS             22684..23379
                     /locus_tag="OO327_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR6018023.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OO327_001425"
     CDS             complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OO327_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR6018024.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24044..25420
                     /gene="zraS"
                     /locus_tag="OO327_001426"
     CDS             24044..25420
                     /gene="zraS"
                     /locus_tag="OO327_001426"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR6018025.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25417..26742
                     /gene="zraR"
                     /locus_tag="OO327_001427"
     CDS             25417..26742
                     /gene="zraR"
                     /locus_tag="OO327_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCR6018026.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OO327_001428"
     CDS             complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OO327_001428"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR6018027.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFAAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OO327_001429"
     CDS             complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OO327_001429"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR6018028.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7027 a         7824 c         8255 g         6906 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggc ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cattgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacttggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggcagg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgtgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgt ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccaa gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgattg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5161 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat tctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaatatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg gtccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgacgcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcact gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacattgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgta ctgggtctgt actacatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgctaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10141 ttctggtgca tctgttggca tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcaaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag gggggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatattctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacacct tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgtaaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca
    12541 cggaaaatat taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat
    12601 tcctgctgac ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat
    12661 cttccgtagc ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag
    12721 gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac
    12781 acggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg
    12841 tgattatcgg catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc
    12901 agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc
    12961 tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc
    13021 gacgcaggct caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg
    13081 ctttgccagt aatgctgttg cagccatagc aaatgttcgg caaccatata gcagtcaacg
    13141 cgccagttgt cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc
    13201 cccagccgat ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcaaatga
    13261 tggcgggtat aagtcgcctc gtgatatgtc tcctgataaa ccatcacccc atccagacca
    13321 agttgcttta actcggcgta ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa
    13381 gagaactgtt cacgcagggc ggggagatga cgacgaaagt aatccatccc cactttcgcc
    13441 tgatgttcac cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt
    13501 tccctggcaa tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat
    13561 ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta
    13621 actgtgttgc caaatcgctg acgggtcaga cgctgcgcct gttgggccag ttgttccaga
    13681 tagccactgg cggcaggcga taacagcgcc atcatgtcgt cgcgggtgag ttgcgaggca
    13741 tttagcgccc gctctacgtc agcagccgtt ttgccgttga tacgcaggcg gatgtcgtcc
    13801 cagtccagtt gtcgccagcg attgctgaag gttttcatgc cgatgcctcc agaaatccgg
    13861 tcagcgggct ggtggcatga gcaaaataac tgcggctgcc cggtccggac tgacgtgcca
    13921 gcaggcctac ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcga
    13981 cggcaatcgc cgtattcact aacaccgcgt cggcccccat ttccagcgcc tgcgtggcat
    14041 ggctgggaac gccgatgcca gcatcgacaa ccaccggaac tgtggcctgc tggataataa
    14101 tctccagcat ggcacgggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca
    14161 tcaccgctgc acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt
    14221 aaggcagcac gacaaatccc tgttgtacca gcgtttcggc ggctttcagg gtttcgatgg
    14281 gatcgggcaa cagccagcgg gcgtcagggt gaatctccaa ttttagccag tttgtgccta
    14341 acgcttcacg tgccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggaag
    14401 tattcggcag cagggccaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct
    14461 ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg
    14521 cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa
    14581 acgttttgtc cgcaatacgt aacatctcaa ccccctgcaa taacctgaaa aagcaggatc
    14641 tggtcgccat cctgcacgat atgttgcgtc cactgctcac gcgggacgat ttgctgatta
    14701 atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt
    14761 tgcccggcgg cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca
    14821 taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc cactggctcg acttaccgtc
    14881 gaacagtcgg agttctcccg caggcgtctc tataccgctt aataacttaa tggcttccag
    14941 tgcctgcaaa gtgcccatca ccccgaccac cgggccaacc acgcccgccg tgcggcagtt
    15001 gcgttctggc tgctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg
    15061 tgtcagtacc atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt
    15121 gagtgccacg caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag
    15181 caccacatcg gcttgtgcaa ccgcatcttt caacgcctca cccgttaacc gttgttgtaa
    15241 tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc tgttggctga cctgcgattt
    15301 cgggctgttg atatcttcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac
    15361 atcgtcgtca tctgccagta ccagcgtccc gacgccagcc cctgccaggt atagcgcagc
    15421 aggtgtaccc agcccgccaa gaccaataat cagcacctgg ctgtcgagca gtttttgctg
    15481 cccgtcaaga gcgatatcgt cgagcagaat ttggcggcta taacgcataa agtcacggtc
    15541 attcatcgcc aactcctgca atttccagca actgtgccgt tgccaaacgc cagtctgcgg
    15601 cttgagtaat ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg
    15661 cgcgtgccag actgataccg ccaatcgcca cggtgggata atccgccagg cgctcaacat
    15721 gccgtgccaa ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga
    15781 acacatgtcc cagcgcgata taagaggggc gtgctgccag cgcgacgtcg atttccatat
    15841 cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg gatggcattg agatcggtgg
    15901 cttgcaaatc ttcctgcccc aaatggacgc catacgcctg atgcttgatc gcgagccgcc
    15961 agtaatcgtt gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacga
    16021 catcggcttc cacctcttca tcgcgccgat ctttgatgcg tagctggaga gtacgtacgc
    16081 ctgcatccaa cagacgttcg atccactgta cgctgtccac caccgggtac agtcctaaac
    16141 gaaaaggtac aggaggaaaa tcaggctgat acatcacgct tcctccttat gcaggtagat
    16201 ttctccgcct ctggcacgga agttctccga catatccgcc attcccactt caatggtttg
    16261 cgcggcggcg taatcgcgta cttcctggct gattttcatc gagcagaatt ttggcccaca
    16321 catggagcaa aaatgggcga ctttgccgga ctcttggggc agggtttcat cgtgataagc
    16381 gcgggcggta aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg
    16441 ggctttcgac atggcgttat cgcgaatttg cgcgcccgga tgcccttttg ccaggtcagc
    16501 agcgtgggcg gcaatcttat aagtgataag cccctgctta acatcttctt tattgggcag
    16561 acccagatgc tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc agccaatcat
    16621 cgccgcacca atccccgacg tgaagtggtc atagcccggt gcaatatcgg tagttagcgg
    16681 ccccagagtg taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggcg
    16741 gatcatctgc atcggcacat ggcctgggcc ttcaatcatc acctgtacgt catattccca
    16801 ggcgattttg gtcagttcgc ccagcgtatg caattcggca aactgcgctt catcgttggc
    16861 gtcctgaata gaaccggggc gcagaccgtc gcccagcgac agcgaaacgt cataagcggc
    16921 acagatttca caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag
    16981 gcaccatttc gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt
    17041 catcggcaca tagcgcagca gtacgcccgc atggatagtg aagtaatcca caccttgctc
    17101 ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt
    17161 aaccttctcc agcgcctggt agatcggcac tgtaccgatc ggcaccggac tgttacgcaa
    17221 aatccactcg cgggtttcgt gaatatagcg accggtggag agatccatca ccgtatccgc
    17281 tccccagcgc gtggaccata ccagcttttc cacttcttct tcgatggaag aggtgaccgc
    17341 cgagttgccg atattggcgt taacttttac caggaagtta cggccaataa tcatcggctc
    17401 cgattccgga tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg
    17461 gacaaattcc gcagtgatat tttccggcag atgtgcgcca aagctcattc ccggatgctg
    17521 gtggcgtaaa acctcgctac ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa
    17581 ttccatttcc ggcgtgatga tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc
    17641 tgttttggcg cgttttggcg ttaataagcc gctaaaacgt agctcgtcga gaccatcatc
    17701 tgccagccgc gttctggtgt aatcggaact gcgcacggta agttcttcgg tatcggcgcg
    17761 cgcatcgatc cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcgatctg
    17821 cggatcacca tacgggccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg
    17881 cggctgttct ttgctaccgc caattagcgt cgggctaagc tggatctcac gcatcggcac
    17941 gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt gagttgggaa aggcggtgcc
    18001 ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt
    18061 tgcagacata gctcattcca aaaagttaag gacgtggctt gtcagacgac ggatgaagca
    18121 agagacgatc gccgcaaagg cgatgcgata gcagattaac tcttgttccc ttcgcaggta
    18181 ttagcctgat caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg
    18241 acaagaaatt agcccccaca aacggggcgt tgaatgtaaa ttacgcgtta acagcgcaga
    18301 actcaagcag gatgtttgac gcgggcggcg tcaagcacca gcagaatcag cttatcttcc
    18361 agcacaaagc gcgcttccag cgcctcgccg atgtcagata aaacctgttg aaactcaagg
    18421 taattatcat gatcgatggc ggtttccagg ctggaatcgt agtaatccat aatctgttgg
    18481 gtattggctt cgagttgcgg ccaaatcttc gcggcgcgcg cgagttgccc gttgccttcc
    18541 agcttatgaa gaatgcgctc ataaatactg aaatgtccgg cagacaagta atcgaccagg
    18601 ctctgacaaa aatcatcaag ggctttttcg tttagcctca tgtacgattc tttgccaggc
    18661 ttaatgccaa ccagattgta gtaagccacg agcagatgct tacgtacatg tagccagcga
    18721 tcaaccagtt tgttacttcc tctgacgcgc tccgtcaggt tatcgagctg gttaagcatg
    18781 attgactccg caagtttgta ttcaaaaact gctcagtgag aaatgtaaaa accatgttaa
    18841 acatgccagt gatgcaaagg tagtgcaaga gctatggatc gtataattga aaaattagat
    18901 cacggctggt gggtcgtcag ccatgaacaa aaattatggt tgccgaaggg agaattgcca
    18961 tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg cactacaaat cggcgaatgg
    19021 cagggggaac ctgtttggtt aatacaacag cagcggcgtc acgatatggg gtcggtacgt
    19081 caggtcattg atctcgatgt tgggctgttt caactggccg gacgtggcgt gcaactggcg
    19141 gagttttacc gatcgcataa atactgtggt tactgcgggc atgaaatgta tccgagcaaa
    19201 accgaatggg cgatgctgtg tagccattgc cgtgagcgtt actacccgca aatcgccccc
    19261 tgcattattg ttgccatccg tcgcgatgat tcgctcctcc tcgcccagca tacccgccat
    19321 cgtaacggtg tccatacagt acttgccgga ttcgtcgaag tgggcgaaac cctcgagcag
    19381 gcagtcgcgc gggaagtgat ggaagagagc ggaattaaag ttaaaaactt gcgttacgtg
    19441 acttctcagc cgtggccgtt tcctcagtct ttaatgaccg cgtttatggc ggaatatgac
    19501 agcggcgaca tcgtgatcga cccgaaagaa ttgctcgagg cgaactggta tcgctatgac
    19561 gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc gtctgataga agatactgtg
    19621 gcgatgtgtc gggcagagta tgagtgatga tacactgacc gcctgacgca ctaaggaaca
    19681 gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg cgctgctgcg ccagcccgtt
    19741 gatgtcactc cagtatggat gatgcgccag gcgggtcgct atctaccgga atataaagcc
    19801 acgcgcgccc aggcgggcga ttttatgtcg ctgtgcaaaa acgccgagct ggcgtgcgaa
    19861 gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg cgatcctctt ttccgatatc
    19921 ctcaccgtgc cggacgcgat ggggttaggg ctctattttg aagccggaga aggtccgcgt
    19981 tttacctcgc cagtcacctg caaagccgac gtcgataaac tgccaattcc ggacccggaa
    20041 gatgagctgg gttacgtgat gaacgcggtg cgtaccattc gtcacgaact gaaaggcgaa
    20101 gtgccgctga ttggtttttc cggcagcccg tggacgctgg caacctacat ggtggaaggc
    20161 ggcagcagca aagccttcac cgtgatcaaa aaaatgatgt atgccgatcc gcaggcgctg
    20221 cacgctctgc tcgataaact ggcgaaaagc gtcactttgt atctgaatgc gcagattaaa
    20281 gccggtgctc aggcagtgat gattttcgac acctggggcg gcgtgcttac cgggcgcgat
    20341 tatcaacagt tctcgctcta ttacatgcat aaaattgttg atggtttact gcgtgaaaac
    20401 gacggtcgcc gcgtgccagt cacgctgttt accaaaggcg gcggacagtg gctggaagcc
    20461 atggctgaaa ccggttgcga tgcgctgggc ctcgactgga caacggacat cgccgatgcg
    20521 cgccgccgtg tgggcaataa agtcgcgttg cagggtaata tggatccgtc gatgctgtac
    20581 gcgccgcctg cccgcattga agaagaagta gcgactatac ttgcaggttt cggtcacggc
    20641 gaaggtcatg tctttaacct tggtcacggc attcatcagg atgtgccgcc agaacatgct
    20701 ggcgtgttcg tggaggcagt gcatcgactg tctgaacagt atcaccgcta agagcctgat
    20761 ccatcaggct attttatttg ccattttgag gccgggcagt gctcaaaatc ctcacgtact
    20821 acgtgtacgc tccggttttt ccgcgctgtc cggcctcaaa ctgccttcaa caattacgcc
    20881 tgttgaacca ggctctaagg agcgattatg gatctcgcgt cattacgcgc tcaacaaatc
    20941 gaactggctt cttctgtgat ctgcgaggat cgactcgata aagatccacc ggatctgatc
    21001 gccggagccg atgtcgggtt tgagcagggc ggagaagtga cgcgagcggc gatggtgctg
    21061 ctgaaatatc cctcgcttga gctggtggag tataaagttg cccgcatcgc caccaccatg
    21121 ccttacattc caggttttct ttccttccgc gaatatcctg cgctgctggc agcgtgggag
    21181 atgctgtcgc aaaagccgga tttagtgttt gtcgatggtc atgggatctc gcatcctcgc
    21241 cgtcttggcg tcgccagcca ttttggctta ttggtggatg tgccgaccat tggcgtggcg
    21301 aaaaaacggc tctgcggtaa attcgaaccg ctctccagca aaccgggcgc gctggcccca
    21361 ctgatggata aaggcgagca gctggcctgg gtctggcgca gcaaagcgcg ctgtaacccg
    21421 ttgtttatcg ctaccggcca tcgggtcagc gtggacagcg cgctggcgtg ggtacaacgc
    21481 tgcatgaaag gctatcgtct gccggagcca acgcgctggg cggacgcggt ggcctcggaa
    21541 cgtccggcgt tcgtgcgcta tacagcaaat cagccctaat tcaggtaaac tgcggataat
    21601 ttccgtattt gagaactcaa catgttacaa aacccaattc atctgcgtct ggagcgcctg
    21661 gaaagctggc agcatgtcac tttcatggct tgcttatgcg aacgcatgta ccccaattac
    21721 gccatgttct gccagcaaac cggttttggt gatgggcaaa tttaccgtcg tattctcgat
    21781 ctcatctggg aaacgctgac cgttaaagac gcaaaagtaa atttcgacag ccaactggag
    21841 aaatttgaag aagcgattcc ttcagccgac gatttcgatc tgtacggcgt ttatccggca
    21901 atcgatgcct gcgtggcgtt aagtgaactg gtccattcgc gtttgagtgg tgaaacgctc
    21961 gaacacgcgg tggaagtgag taagacctcc atcacgaccg ttgcgatgct ggaaatgact
    22021 caggctggtc gcgaaatggg cgatgaagag cttaaagaaa acccagctgt agagcaagaa
    22081 tgggacattc agtgggaaat attccgactt ttagctgagt gcgaagaacg cgatatcgag
    22141 ctgataaaag gccttagggc agacctgcgt gagtcgggtg agagcaatat tggtataatt
    22201 tttcagcaat aagaccagaa aacgtgattt aacgcctgat ttgtcgtacc tggagtcttc
    22261 cctttcgccc cccgtctggt ctacatttgg ggggcgaaaa aaagtggcta tcggtgcgtg
    22321 tatgcaggag agtgctattc tggcatttcc gtcgcactcg atgcttagca agcgataaac
    22381 acattgtaag gataacttat gaacaagact caactgattg atgtaattgc agagaaagca
    22441 gaactgtcca aaacccaggc taaagctgct ctggagtcca ctctggctgc aattactgag
    22501 tctctgaaag aaggcgatgc tgtacaactg gttggtttcg gtaccttcaa agtgaaccat
    22561 cgcgctgagc gtactggccg caacccgcag accggtaaag aaatcaaaat tgccgcagct
    22621 aacgtaccgg catttgtttc tggcaaggca ctgaaagacg cagttaagta agattgcgtg
    22681 gcagtgaaca gttttaacga aggggtggtt tcaccccttt tgtctttctg gcgtcgatca
    22741 ttgatgctgg caggtgcgct gtttctcact gcctgtagcc acaactcttc acttcctccc
    22801 tttaccgcca gtggatttgc tgaagaccag ggcgcggtac gcatctggcg aaaagacagc
    22861 ggcgataatg tgcatctgct tgccgtgttt agcccgtggc gcagtggcga taccacgacg
    22921 cgagagtatc gctggcaggg cgataacctc acgctcatca atatcaatgt ttacagcaaa
    22981 ccgccggtga acattcgcgc gcgttttgac gatcgcggcg atctgagctt tatgcaacgt
    23041 gaatccgatg gggaaaagca gcagctttct aacgaccaaa tcgatttata ccgttatcgt
    23101 gctgatcaga tccgccagat tagcgatgcc ttacgtcagg ggagagtcgt gctgcgccag
    23161 gggcgctggc atgcgatgga acagaccgtg accacctgcg aagggcaaac cattaaacct
    23221 gatttagatt cgcaggcgat agcgcatatc gagcgccgcc agagccgctc ttctgttgat
    23281 gtcagcgtgg catggctgga agcgcccgaa ggttcgcaat tactgttagt ggcaaactct
    23341 gatttctgtc gctggcaacc caacgagaaa acgttctgat ttaccagtgg cccataccca
    23401 tatgaccgcc accaccgcag ccgccgtagc ccatgcccat tccggctccg cgcggaatac
    23461 ccgcttcagc catcgcgata tctcgtttca cccgtaactc atctaacgac tgacgcaaat
    23521 tctccatctc tttggcgacc gcgttaattt tgctgctatc cggtgggttc gcggctaaca
    23581 gggcattgta ttcataacgc ttcgtcacca gttgctgttg cagtgcgctg ctttgagcgt
    23641 aaaagtcatt atggattttc tgccacgctg tctgttgttc gctggtcaaa ggcgcggcat
    23701 tttgctgcca cataccgtgt ccgccgtgag caaatgcaga tgtcgatccc atcgccattg
    23761 ctgaaagcgc catcattacc agggcaattt tcgtgttccg tttcatggtt aatcctccag
    23821 tggttgtctt acttcgggta ttgcatcttc cgtgccaacg atgaaacgct gatatgacgg
    23881 gtaatctggc atgataaacg agtaaaaatg actcgcctgc tgcgggtagc gagtcatttt
    23941 tactcattga aacttaagcc tttgtgttac agcgcagggt aagcgctgat aaaagatggc
    24001 atgatttctg ctgtcagaaa gggatgagca ggcaaagaag aagatgcgtt ttatgcaacg
    24061 ttctaaagac tccttagcta aatggttaag cgcgatcctc cccgtggtca ttgttgggct
    24121 ggtagggttg tttgcggtga cggtgattcg cgattatggg cgcgagactg ccgccgccag
    24181 acaaacgctg ctggaaaaag gcagtgtact tattcgcgct cttgaatccg gctcgcgcgt
    24241 cggcatgggg atgcgcatgc atcatgcgca gcagcaggcc ttactggaag aaatggccgg
    24301 gcagcctgga gtacgttggt ttgcggtcac ggatgaacaa ggaacaatcg tgatgcatag
    24361 caactccggc atggtgggaa aacagcttta ttcccctcag gaaatgcagc agttacatcc
    24421 gggagatgaa gaagcgtggc ggcggatcga tagcgcagac ggcgagcctg ttctggaaat
    24481 ttatcgccag tttcaaccga tgtttgctgc tggaatgtac cggatgcgcc atatgcagca
    24541 gtatgccgcg acaccacaag caattttcat cgctttcgac gccagtaata ttgtgagtgc
    24601 cgaagatcgt gagcagagaa acaccctgat tattctcttc gccctggcga cggtcttgct
    24661 ggcaagcgta ttgtcattct tctggtatcg ccgctatctg cgctcgcgcc agcttctaca
    24721 agatgaaatg aagcgcaaag agaagctggt ggcgctgggg catcttgcgg caggcgttgc
    24781 ccacgaaatc cgtaacccac tttcctcgat taaaggactg gcgaaatact ttgccgagcg
    24841 cgcgcctgca gggggagaag cgcatcaact ggcgcaggtg atggcgaaag aggccgaccg
    24901 tttaaaccgc gtggtaagcg agttgctgga actggttaag ccaacgcatc tggctttgca
    24961 ggcggtggat ctcaacacgc tgattaacca ctcattacag ctggtaagcc aggatgcaaa
    25021 cagccgggag atccagttac gctttaccgc caacgacaca ttaccggaaa ttcaggccga
    25081 tccggacagg ctgactcagg tcctgttgaa tctctatctc aatgctattc aggcgattgg
    25141 tcagcatggc gtgattagcg tgacggccag cgaaagcggc gcgggcgtga aaatcagcgt
    25201 taccgacagc ggtaagggaa ttgcggcaga tcagcttgaa gccatcttca ctccgtactt
    25261 caccaccaaa gccgaaggca ccggattggg gctggcggtc gtgcataata ttgttgaaca
    25321 acacggtggt acaattcagg tcgcaagcca ggagggaaaa ggctcaacgt tcaccctctg
    25381 gcttccggtc aatattacgc gtaaggaccc acaaggatga cgcacgataa tatcgatatt
    25441 ctggtggtgg atgatgacat tagccactgc actattttgc aggctttact gcgcggctgg
    25501 ggctataacg tcgcgctggc gaacagcggg cgacaggcgc tggagcaggt gcgggaacag
    25561 gtttttgatc ttgtgctttg cgatgtgcga atggcggaga tggacggcat cgccacgctg
    25621 aaagagatca aagcgttaaa cccggcaatt ccggtgctga ttatgactgc gtactccagc
    25681 gtcgagacgg cggtagaggc actgaaaact ggggcgcagg attatctcat caagccgctg
    25741 gatttcgata acctacaggc gacgctggaa aaggcgctcg cgcatacgca cagtattgat
    25801 gctgaaacac ctgcggtgac tgccagccag ttcggtatgg tcggtaaaag cccggcgatg
    25861 caacacctgc tcagtgaaat cgccctcgtc gcgccatcgg aagccacggt gctgatccac
    25921 ggcgattccg gcaccggtaa agagctggtc gccagggcga ttcacgccag tagcgcacgt
    25981 agcgaaaaac cgctggtaac gctcaactgt gcggcactca acgaatcctt gctggaatct
    26041 gaattgttcg gtcacgaaaa aggggcgttt actggagccg ataaacgccg ggaagggcgc
    26101 tttgttgagg cggacggcgg cacgctgttt ctcgatgaaa ttggcgatat ctcgccgatg
    26161 atgcaggtgc gtctgctgcg tgcgattcag gagcgcgaag ttcagcgtgt cggtagcaac
    26221 cagactatct cggttgatgt ccggctgatt gcggcgaccc atcgcgatct tgccgcagag
    26281 gtgaatgccg ggcgttttcg ccaggatctc tactatcgcc tgaatgtggt ggcgattgaa
    26341 gtaccatcgc tgcggcaacg gcgggaagat atccctctgc tggctggcca ttttcttcag
    26401 cgctttgccg agcgtaatcg caaggcggta aaaggcttta cgccacaggc gatggatttg
    26461 ctgatccact acgactggcc gggaaatatt cgtgaactgg aaaacgcggt ggaacgggcg
    26521 gtggtgctgc tgaccgggga atatatttcc gaacgcgagc tgccgctggc gattgccagt
    26581 acgccgatcc cgctggggca aagtcaggat attcagccgc tggtggaagt ggaaaaagag
    26641 gtgattctgg cggcgctgga gaaaacgggc ggcaacaaaa ccgaagccgc ccgtcagtta
    26701 gggatcacgc gcaaaacgct attggcaaaa ctgtcgcgtt agttctgctc gcgttcgatg
    26761 gcgcgccaac cgatatcttt ccggcagaag caatcatccc agtggatatc ggtcattaag
    26821 gcgtaggcgc gtttctgcgc ttctgccacg gtatgaccca gcgcggtgac gcacagtacg
    26881 cgcccgccgc tggttaccac ctgctcgtca tccgccagtt ttgtgcccgc gtggaacact
    26941 ttgccgtcag ccacttcttc cagcggcagg ccgtggatca catcgccggt gcggtaatcg
    27001 cccggatatc cacccgcagc catcaccacg ctgagagaag cacgttcatc ccactctgac
    27061 gttttctcgt ccagtttgcc ttcacaggcc gccaggcaga gctcaaccag atcggacttc
    27121 atgcgcagca taatcggctg ggtttccgga tcgccaaagc ggcagttaaa ttcaataacc
    27181 ttcggattgc cctgtttgtc gatcatcagg cccgcataga gaaaaccggt gtaggtgttg
    27241 ccttccgccg ccatgccttt cacggttggc cagatgatac gttccatggt gcgctgatga
    27301 acttcatcgg ttactaccgg cgcgggggag taagcgccca tcccgccggt gtttggtccg
    27361 ttatctttat cgcctacgcg tttgtgatcc tggctggtag ccatcggcag cacatgctcg
    27421 ccgtccacca tcacgataaa gctcgcttct tcgccatcga ggaactcttc gataacgatg
    27481 cgatgacccg cgtcgccaaa agcgttgccc gccagcatat cgtgaacagc cgcttccgct
    27541 tcttccagcg tcatcgccac gataacgcct ttcccggcag ccagaccgtc cgctttaatg
    27601 acgattggcg cgcctttctc acgcagatac accagcgcag gttctacctc ggtgaagttc
    27661 tggtattccg ccgtagggat cttatggcgg gccaggaaat ctttggtaaa cgcttttgag
    27721 ccttccagtt gggccgcacc tgcggttggg ccgaagattt tcagcccggc ggcgcggaag
    27781 gtatcgacca cgcctttcac cagcggcgct tccgggccga cgatggtcag atcaatcttt
    27841 tcgttttgtg cgaaatccaa cagcgccggg atatcggtca cgccgatagc aacgttttgc
    27901 agcgcgggtt ccagcgcagt gcctgcatta cccggagcag caaaaacagt ctcaaccagc
    27961 ggcgactggg ccgctttcca ggccagcgcg tgctcgcgcc cgccgttacc aatcactaat
    28021 actttcatct attgctccat taatggcgga agtggcgcat gtcggtgaag agcatcgcaa
    28081 taccgtgctc gtcggcggcg gcaatcactt cgtcatcacg gatggaaccg ccaggctgga
    28141 ttacgcaggt cacgcccgca gcggcggcgg catcaatacc gtcgcggaac gggaagaacg
    28201 cgtcagaagc catcgaggaa cctttcactt ccaggccttc atcggcagct ttaataccgg
    28261 cgattttcgc ggagtacacg cggctcatct ggcccgcgcc aataccgatg gtcatattgt
    28321 ttttggcata gacgatagca ttggatttca cgaatttcgc cactttccag cagaacagcg
    28381 catcacgcag ttcctgttcg gtcggctgac gttgggtgac gacgcgcagt tcttccgcac
    28441 cgaccatccc caggtcacga tcctgaacca gcagaccgcc gttcacgcgt ttgaaatcaa
    28501 gacccggaac acgctcgccc cactgaccgc aggtcagaac gcgtacgttc tgcttggcgg
    28561 cggtgatttt cagggcttct tcgctggcgg aaggcgcaat aatcacttcg acaaactgac
    28621 gagaaatgat ggcctgtgcg gtttccgcat ccagctcgcg gttaaaggca atgatgccgc
    28681 cgaatgcgga ggttgggtcg gttttgtacg cgcgatcgta agcatcaaga atggaattgc
    28741 cgatagctac accgcaaggg ttggcgtgct tcacaatcac acatgccggc tcggcgaact
    28801 ctttcacgca ctccagcgcc gcatcggtat cggcgatgtt gttataagag agggctttac
    28861 cctgaacctg ggttgcggta gcaacggagg cttctttcac attctcttct atatagaagg
    28921 cagcctgctg gtggctgttc tcgccgtaac gcatatcctg cttcttaatg aagttcaggt
    28981 tcagcgtgcg tgggaagcga ccggcggctt ctttgctttc accgtggtac gccggaacca
    29041 tgctgccgaa gtagttggca atcatgctgt cgtaggcggc agtgtgttcg aaggctttga
    29101 tggcgaggtc gaaacgggtt gcaagcgtca gcgatccttc gttgtcatcc atctctttaa
    29161 taatggcgtc gtagtcgctg ctcttcacca cgattgcgac atctttatgg ttcttagcgg
    29221 cggagcgcac catggtcggg ccgccgatat cgatgttctc aaccgcatct tccagcgagc
    29281 agccttcacg agcaacagtc tgggcgaacg gatacaggtt aacaaccacc atatcgatag
    29341 gctggatctg atgttcttcc ataatggcat cgtcctggcc gcgacggccc agaatgccac
    29401 catgtacttt cggatgcagg gtcttcacgc gtccatccat catctccggg aaaccggtgt
    29461 aatcggaaac ttcggttacc ggcagacctt tttctgctaa cagacgggca gtgccccctg
    29521 tagacagcag ctccacaccg cgtgcggaaa gtgcctgggc gaattcgacg ataccggctt
    29581 tgtcagaaac actgagcaga gcgcggcgga ctggacgacg ttgttgcatg gtaaatcccc
    29641 tgggtttgac tattacagag agcgttagct gaatttttcg cgaaaaactc agctaacgcc
    29701 cctaacgggg catccttatt tttcgcccgc attgtaacga aaacgtttgc gcaacgctcg
    29761 cgaatttttc tctttcaatg gtgatcacaa ttttgacagt ggttaccgtg ggaaaaatac
    29821 agaaattaca ttgatgattg tggataactc tgtgcgtaaa aaggtataaa gcggggtttt
    29881 gctggggaat gcagcagtca gtcatttttc tgcaattttt ctattgcggc ctgcggagaa
    29941 ctccctataa tgcgccttca tcgacacggc ggatgtgaat cacttcacac aaacagccgg
    30001 ttcggttgaa ga
//
LOCUS       DAJTSI010000036        29970 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000036 DAJTSI010000000
VERSION     DAJTSI010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29970
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.036"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            281..457
                     /locus_tag="OO327_001430"
     CDS             281..457
                     /locus_tag="OO327_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018029.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            444..668
                     /locus_tag="OO327_001431"
     CDS             444..668
                     /locus_tag="OO327_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR6018030.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(701..1321)
                     /gene="sdiA"
                     /locus_tag="OO327_001432"
                     /pseudo
     CDS             complement(701..1321)
                     /gene="sdiA"
                     /locus_tag="OO327_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(1551..2303)
                     /gene="yecC"
                     /locus_tag="OO327_001433"
     CDS             complement(1551..2303)
                     /gene="yecC"
                     /locus_tag="OO327_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR6018031.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(2300..2968)
                     /gene="tcyL"
                     /locus_tag="OO327_001434"
     CDS             complement(2300..2968)
                     /gene="tcyL"
                     /locus_tag="OO327_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR6018032.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(2983..3969)
                     /gene="dcyD"
                     /locus_tag="OO327_001435"
     CDS             complement(2983..3969)
                     /gene="dcyD"
                     /locus_tag="OO327_001435"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR6018033.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(4074..4874)
                     /gene="tcyJ"
                     /locus_tag="OO327_001436"
     CDS             complement(4074..4874)
                     /gene="tcyJ"
                     /locus_tag="OO327_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6018034.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(4962..5513)
                     /gene="fliZ"
                     /locus_tag="OO327_001437"
     CDS             complement(4962..5513)
                     /gene="fliZ"
                     /locus_tag="OO327_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR6018035.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(5559..6278)
                     /gene="fliA"
                     /locus_tag="OO327_001438"
                     /pseudo
     CDS             complement(5559..6278)
                     /gene="fliA"
                     /locus_tag="OO327_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(6443..8095)
                     /gene="fliC"
                     /locus_tag="OO327_001439"
     CDS             complement(6443..8095)
                     /gene="fliC"
                     /locus_tag="OO327_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR6018036.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            8361..9767
                     /gene="fliD"
                     /locus_tag="OO327_001440"
     CDS             8361..9767
                     /gene="fliD"
                     /locus_tag="OO327_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR6018037.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKVQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            9792..10202
                     /gene="fliS"
                     /locus_tag="OO327_001441"
     CDS             9792..10202
                     /gene="fliS"
                     /locus_tag="OO327_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR6018038.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            10202..10567
                     /gene="fliT"
                     /locus_tag="OO327_001442"
     CDS             10202..10567
                     /gene="fliT"
                     /locus_tag="OO327_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCR6018039.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            10645..12132
                     /gene="amyA"
                     /locus_tag="OO327_001443"
     CDS             10645..12132
                     /gene="amyA"
                     /locus_tag="OO327_001443"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR6018040.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(12166..12579)
                     /locus_tag="OO327_001444"
     CDS             complement(12166..12579)
                     /locus_tag="OO327_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6018041.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            12766..13971
                     /gene="yedE"
                     /locus_tag="OO327_001445"
     CDS             12766..13971
                     /gene="yedE"
                     /locus_tag="OO327_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR6018042.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            13968..14201
                     /gene="yedF"
                     /locus_tag="OO327_001446"
     CDS             13968..14201
                     /gene="yedF"
                     /locus_tag="OO327_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR6018043.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            14308..14978
                     /locus_tag="OO327_001447"
                     /pseudo
     CDS             14308..14978
                     /locus_tag="OO327_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(15018..16226)
                     /locus_tag="OO327_001448"
                     /pseudo
     CDS             complement(15018..16226)
                     /locus_tag="OO327_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            16294..16909
                     /gene="tnpA"
                     /locus_tag="OO327_001449"
                     /pseudo
     CDS             16294..16909
                     /gene="tnpA"
                     /locus_tag="OO327_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            17237..18319
                     /locus_tag="OO327_001450"
     CDS             17237..18319
                     /locus_tag="OO327_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HCR6018044.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(18808..19606)
                     /locus_tag="OO327_001451"
                     /pseudo
     CDS             complement(18808..19606)
                     /locus_tag="OO327_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(19616..20167)
                     /gene="ybcL"
                     /locus_tag="OO327_001452"
     CDS             complement(19616..20167)
                     /gene="ybcL"
                     /locus_tag="OO327_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCR6018045.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(20336..20668)
                     /gene="emrE"
                     /locus_tag="OO327_001453"
     CDS             complement(20336..20668)
                     /gene="emrE"
                     /locus_tag="OO327_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCR6018046.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(21013..21327)
                     /gene="fliE"
                     /locus_tag="OO327_001454"
     CDS             complement(21013..21327)
                     /gene="fliE"
                     /locus_tag="OO327_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR6018047.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            21541..23200
                     /gene="fliF"
                     /locus_tag="OO327_001455"
                     /pseudo
     CDS             21541..23200
                     /gene="fliF"
                     /locus_tag="OO327_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            23193..24188
                     /gene="fliG"
                     /locus_tag="OO327_001456"
     CDS             23193..24188
                     /gene="fliG"
                     /locus_tag="OO327_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR6018048.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            24181..24867
                     /gene="fliH"
                     /locus_tag="OO327_001457"
     CDS             24181..24867
                     /gene="fliH"
                     /locus_tag="OO327_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR6018049.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            24867..26240
                     /gene="fliI"
                     /locus_tag="OO327_001458"
     CDS             24867..26240
                     /gene="fliI"
                     /locus_tag="OO327_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCR6018050.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            26262..26603
                     /gene="fliJ"
                     /locus_tag="OO327_001459"
                     /pseudo
     CDS             26262..26603
                     /gene="fliJ"
                     /locus_tag="OO327_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            26600..27727
                     /gene="fliK"
                     /locus_tag="OO327_001460"
     CDS             26600..27727
                     /gene="fliK"
                     /locus_tag="OO327_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR6018051.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            27832..28296
                     /gene="fliL"
                     /locus_tag="OO327_001461"
     CDS             27832..28296
                     /gene="fliL"
                     /locus_tag="OO327_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR6018052.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            28301..29305
                     /gene="fliM"
                     /locus_tag="OO327_001462"
     CDS             28301..29305
                     /gene="fliM"
                     /locus_tag="OO327_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR6018053.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASISGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            29302..29715
                     /gene="fliN"
                     /locus_tag="OO327_001463"
     CDS             29302..29715
                     /gene="fliN"
                     /locus_tag="OO327_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR6018054.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            29718..>29970
                     /gene="fliO"
                     /locus_tag="OO327_001464"
     CDS             29718..>29970
                     /gene="fliO"
                     /locus_tag="OO327_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HCR6018055.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTA"
BASE COUNT         7602 a         7615 c         7405 g         7348 t
ORIGIN      
        1 aatagggata acgctactga tagttagtca ttcgtatatt agcggttttt attgcaactt
       61 tgaaacgtta aaaatggtgg gtattcgatt tttctctcgt ttttgtcaat tcgattgtcc
      121 acagttatgg tcacgcccgt caccaacggc ttaccggatc gtttcacagt aagccctacg
      181 atgaaatgtg acaaaaattg actttattca gcaaaaatga aaatcagccc gctgaatatg
      241 taacattaat taacccgaac aatacgggca aaaaacattc atgataaaaa tatttattgg
      301 tcattatatt aacgtatttt atagcactgc cgatatcacg ctcaaaaaac aaccactgct
      361 atttttagca aagcttatgg tatactccgc cgccttaaca tttttcacca caaattttca
      421 ttgcaacatg acgaggaaaa taaatgagta cgcctgattt ttctactgcc gagaataatc
      481 aagaactggc aaatgaagtc tcctgcctga aagcgatgct gacgctgatg ctgcaggcga
      541 tgggacaagc tgacgcgggc cgcgtgatgt taaagatgga aaaacagctt gcgctgatcg
      601 aagacgaaac ccaggctgca gtattttcca aaacggttaa gcaaattaaa caagcctacc
      661 gtcagtaata taaaaccggc tgagagcgtg cctttcagcc ggttattgac aaaatcatcg
      721 ctatctctgc tgatgtcttc ccttccgccg tccatttcag aatttctttt tcgcgcttgc
      781 tgaaattcat ctctggcgtc atcactattt catcatttaa acgcatcaga gccatcagac
      841 tttcgcgcac cagtaactgc atttttaatt gcagttcatc actaagaatg ggtatttcgc
      901 gcgtgctgca acgggaaaag gacaaaaagc ccagcgcccg atttggcagc attaaatact
      961 gagtgacacc gcggcgtaaa ccatgtgcgt gcgcggcttc ccataatggc tgtgcttcgc
     1021 tgaataagtc atcattccac attaaatggc cctgactaaa gttttcaggg ttcagcacag
     1081 gatcaattgc gagaaaattt tttgcctgat aataactaac ccacgactca gggtaattgg
     1141 tgtagaaagc cactttaggt cgcgtgaatg gtaccgggtg gcgaacacat aacgaatagt
     1201 aatcgtactc cagctgctga gcctgaagct ctatttcatg gtaggcctct tctgcggcct
     1261 ccatcctctg aaaacgcaac agcatcgtgc gacgccagct gaaaaaatcc gtatcctgca
     1321 tagtaaaccg caacgcccct gagagtgagt atcatttata atgataattc aaactatcac
     1381 ataaataaga tttatatata atttatatta ttcaggcaat gaattacttt tgcaagccat
     1441 cgcattctct tatgttatta atgagttatg ctgatttgtt aagcagtttt atcacgcttg
     1501 aaatggcgtc ccgccccgac aggtgaatcg tcggggctga ttttttctta ttattgcagc
     1561 agaagcttct cgaggaactg gcgggtgcga ggctgctggg gatcggcaaa taacgctttt
     1621 gctgccccct gctcgactat ccgcccctgg tccataaaga tcgcccggtc cgcaacatcc
     1681 cgggcaaagc tcatttcgtg tgtcacaatc accatcgtgc gcttttcctg cgccagctgg
     1741 cggatggtgt tcaggacttc gcccaccagc tctggatcca gcgccgacgt tggctcgtca
     1801 aacaaaatca cctcaggacg cattgccagt gcacgcgcaa tcgcaacacg ctgctgttga
     1861 ccgccagaca aacgacgtgg atagctggtt tctttaccgg ccagcccaac tttcgccagc
     1921 agctcgcgag cgcgcgccgt ggcctcttct ttcggttcgc ctttgacgat caccggccct
     1981 tcaataatgt tttccagcac cgtacgatgc ggaaacaaat taaagttctg gaagacaaac
     2041 ccgacgtgct gacgcaactg gcgaatcagc gatttttgct gacttaatga acgtgcagta
     2101 tcaatagtga tatcgccaac ggtgatcgtt cccgcttccg gttgttccag cagatttatg
     2161 ctacgtagca atgtggtttt gccggaacca ctcggaccga taattgccac cacttcgcca
     2221 ggctttacct cgaggtcgat accgtgcagc accgtctgac cgtggaattt tttcaccagg
     2281 ttcttaactt caatggcact cattttggtt ctctctcctg gcgattaagt tgattctcaa
     2341 aatggttctg caacgtcgat aacacagtcg ccatgatcca gtagatgagc gaagccgcca
     2401 ggtacatggt gaacacctcc agcgtgcgcg aggtaatcaa ctgcgcctga cggaacagct
     2461 ccggcacctg gattgtcgcg gccagcgacg tatcttttac cagactgata aagctgttcg
     2521 acagcggcgg caacgccacc cgcgccgcct gtggcaaaat cgcgcgacgc atggtctgcc
     2581 acggcgtcat accaatactg gcagcggctt cccactgacc tttatcaata gaagaaatgg
     2641 cggcgcgcag cgtttcggcg gcataggcgg cagtgttcag cgacagacca atcattgctg
     2701 acggaatggg atctaattcg ataccaaact gcggcaggcc gtaatagatc ataaacagtt
     2761 gtgcgatgag cggcgtacca cgaaaaatag agatataaaa acgcgccagc cagcgcacgg
     2821 gccagattgg cgacaggcgc atcagcgcga gaataaaccc gagtagtaag ccaaaaaaca
     2881 tgccgccgat actgagttgc agggtatacc cagccccttt caacaggaac ggcaaggaat
     2941 caataaccag ttgtatactt tcttgcatga gcgttcccgg agctaaacgt ggggatgata
     3001 ggcgaacagc gcaggcgcgc cgccggtatg aataaacaga atcggccctt catctttgaa
     3061 gcgtttctga ctgataccgt caatcagccc cgccatcgct tttccggtat acacaggatc
     3121 aagcagaatg ccttccagac gcgccagcaa tttcactgct tccatgcctt cgtcgttcgg
     3181 cacgccgtag ccaggtgcaa aatagtcatc ccagagtaaa atttccgctg atgcggtcag
     3241 ctccagttct ttcgcaatcg cctgttgtag gttaaccact ttcggcaatt gatcggcaac
     3301 ggaacgcgac acggtcacgc caatcagttc gctttcaggc atcaggtgtt ccagcccaac
     3361 agccagtccg gcgtgagttc cggcactgcc cgatgcgact accaccgacg aaatattaac
     3421 cgccccttca cactgttgcg cgatttccag cgcactctcc acataaccta gcgcgccaag
     3481 agcattagaa ccgccaaccg gaatgacata cgggcgaaag ccttgtgctt cgactcgcgt
     3541 cgccagttct tccagttggg cattgggatc ggtcagtgcg tcgcacattt caatctgggt
     3601 attgaacaga tccagcaaca aacgattgcc gttggttaaa tagttttctg cggttgtgcc
     3661 aataggattt tccagcagcg ccacgcagtg cagaccgagt ttcgccgcga ctgcggcagt
     3721 ctggcgcaca tggttagact ggatcgcgcc ggcagtaatc agcgtatcgg caccttcgcg
     3781 cagggcatct gccgcgagaa attccagctt acgtaattta ttgccgccca ttgccatggg
     3841 ggtgacctca tcccgtttga tgaaaatttc ccgtccaaga taatcagaaa agcgcggcag
     3901 atattcgagc ggcgttggcg cgccgataaa ctccaaccgt ggaaaacggg ttaaattatg
     3961 cagtggcata acagcctccg atgtgtgttg ttgtgatttt cttattatgc acgctgaaaa
     4021 cgcgtaaata aaaaaggcgc tagtgaaagc gccctttttt gtcattatgc tgattatttg
     4081 gtcacatcag caccaaacca tttttcggaa agagcttgca gagtgccgtc tttttgcatt
     4141 tccgcaattg catcattcac cgctttcagc aggtcttcat ttcctttacg cagcgccacg
     4201 ccagactcct gacgggagaa tgcttcaccg gttactgcca gcgtatcgtt ggttttcttc
     4261 accagatcca gcgccgccag acgatcaacg aggatcgcat cgatacgccc tacgcgcaga
     4321 tcctgatatt tggtcgggtc atcatcatag gtacgcacat cgacaccctg aacattctgc
     4381 cgcagccact cttcatagtt ggtgcccaga ccgacaccca cttttttgcc tttcagatca
     4441 gcagccgttt taatggtgcc ttcgttacct tttttcacca gcgcctgaat accggaaatg
     4501 gtgtaagggg tagagaaatc gtattttttc ttgcgctcgt cagaaatggt gacctgatta
     4561 atcaccacat caatgcgttt agagtccagc gacgccagca taccgtccca tttggtcggt
     4621 tttagtgacg cctcaacgcc aagatgtttt gccagctgtt gggcaaattc cacttcaaaa
     4681 ccggttaatt tgccgtcatc cccctgaaaa ctgaacggcg gataagtgcc ttccagccct
     4741 accagcagcg tgccgctctc tttgacttta ttaagcagac cttcatctgc aaaactttta
     4801 acgctcatgc ccgcaaccag cgccacggcc atcacaccca tcaatgcctg acgtcccaga
     4861 tgtgctaatt tcatattcac cccgaatgtt gttatgtctg tttgcagtgt agagccattg
     4921 tttgtaaaca caaaaacaac tccgctacat cttattctta tttaatatat atcagaagca
     4981 ggcaggctgg aggatttctg cacaagtccg gtacaggttt ggcgctgata gtgttgatac
     5041 ttccgtaatg cgatgcggta attgttggtg ctggtggtgg gcagccacgg ggcgaggatt
     5101 tcatcaagga aaccgtcctg cagctgatcg agggaaatat tttgctcatg tagatgatta
     5161 cccaaacggc gcagacgaac gacatattca cgcaccgtac ctgggctcat ttcagtttgc
     5221 tcaaaaagaa atttcttaaa gccgataata tcgaaaaaat cactctgcgt tttgcaatgt
     5281 aaatcaccgc aaaatcggca caatgccgcc catgattgtt gttccgtttg ccagcctttt
     5341 tcatcaagca gcgtgtccag gcgggaaatc tcgattttat tcactatttt gccgtcgcga
     5401 actaaggtaa tgcgatcgag taatttacgg caatgcgcgc aatgggtctg gctgtgttta
     5461 aagtctttaa gatagcggct taatggccgt cttttcaggt gctgcaccat cattaagaac
     5521 tcctggtagt caaagttaaa gtgcggcatt tactgacgtt ataacttacc cagtttagtg
     5581 cgtaaccgtt taatgtcctg gctgtgtaac tgactgaccc gcgattcccc gacctccagc
     5641 accgcgccaa tctctttgag attcagctct tcctggtaat agagggttaa taccagtttt
     5701 tcgcgctccg gcaacgtttc gatggcttcc atcacccgct ggcgcagatt actgtccagc
     5761 agttgttgta gcgggttttc tcgctgatga tcatcagtaa ccagttcgat gctatcgccg
     5821 tgctcttcac gccactcatc gtaggagaag agctggctgt tattggtgtc gagcagcatt
     5881 tgacgataat cggcaatatc gatccctaaa cgttccgcta cctcagtttc cgtggcgttg
     5941 cggccaagtt cctgctccag ttgccctatt gcctgtgcca cttcacgcgc gttgcgtcgc
     6001 acgctgcgcg gcacccagtc acggctgcga agttcatcca gcatagcgcc acggatacgc
     6061 tacactgcgt aagttgtaaa tgccgttcct tgtagggcgt catagcgttc gacagcatta
     6121 agtaacccaa tgccgcccgc ctgtagcaga tcgtcaagtt ccacgctcgc gggcagtcga
     6181 acctgcaggc gcaatgcttc gtgacgcacc agcgggacat aacgctgcca cagcgagtgt
     6241 ttatccatta caccttcagc ggtatagagt gaattcacga taaacagccc tgcgttatat
     6301 gagttatcgg catgattatc cgtttctgca gggtttttaa tcggacgatt agtgggtgaa
     6361 atgaggggtt atttgggggt tacgcaaaaa gccgaatgta aaaaaccccg ccggtggcgg
     6421 ggtttgagag gcattcagac gattaaccct gcagcagaga cagaacctgc tgcggtacct
     6481 ggttggcttt tgccagcacg gagttaccgg cctgctggat aatctgcgct ttcgacatgt
     6541 tggacacttc ggtcgcatag tcggcgtcct gaatacggga ctgcgcttca gacaggttgg
     6601 tagtggtgtt gttcaggttg gtgactgcgg aatccaaacg gttttgcacc gcacccaggg
     6661 aggagcggaa tttgtcgatg gagctgatgg cttcatccag agctttcagg ggatcggctg
     6721 tcgtagctgc ttttgtttca gcatctgtcg tcaatttacc atcagcatct ttataaactg
     6781 ccttaccgct gccgttagtc actgaaccat catcctgtag gtaaaaatct acagtatcag
     6841 atgtggtcaa cgcctcgttg ttatcaacat aaaccgaatc aacacctttt gttacagcgc
     6901 cagttgtgcc gttgacgcta taacctgcag tagtgaaatc aacattgttt cccgaggttg
     6961 atacagcttt atcgaaagct gcctgatcaa cttttgcacc tgttactgaa taagtaatag
     7021 tgtccggcgt agtactatta ccggtaaagg agatagaggt accgccaatg tcaatggttg
     7081 tcgctttggt attattggct gcaagtgtgg acagactagc ctcagtcaaa gaagcaccag
     7141 agccgttttt cgttaagcgc ccagttgtat caatgtagag tttatctcca ttgcttgccg
     7201 taattttacc atcactgctg atctgaacat cctgtgcaga accatcgata gtaatagtgc
     7261 ccttagcggt atcaccaact cccggggtca aatatttctg aacatcggca gctgaagccg
     7321 ttgtggtatc aaaagagtat gacttacttg cactgtcata cttgtaattc gttgcactag
     7381 ccgccgttcc gaagccgtta ttaatggttg cctgaacagt atcaccatca ctaactccag
     7441 ccagcaaatc agacgcttta gcagcatcgt aacccgcact cacagtatat ttgttgccga
     7501 ccactgttgc attagcagct accaagtcag ctttagatgc agcagtatta gccacagccc
     7561 cgccaccatt cacattaaac ccattcagcc ccagcgtatc agagtcaatt ttcttcagat
     7621 caatagtgat agtctggccg tcattcgcac caacctgaat tttcatcgaa ccgtcttttg
     7681 ccagtacgtt cacgccgttg aactgggtct gaccggatac gcggtcaatt tcgtcgagac
     7741 gggatttgat ttcgtcctga atggagtcca gatccgaatc agagttagtc ccggtagaag
     7801 cctgaaccgt cagctcacgg atacgctgta agttgttgtt gatttcggac agcgcgcctt
     7861 cagtggtctg tgcaacagaa ataccgtcgt tggcgttacg tgcagcctga gtcaggcctt
     7921 taatgttaga agtaaaacgg ttagcaatcg cctgacccgc ggcgtcatcc ttcgcgctgt
     7981 taatacgcaa gccagaagac agacgctcga tagaactcga cagcgcagac tggttcttgt
     8041 tgatattatt ttgagtgatc agcgagaggc tgttggtatt aatgacttgt gccatgattc
     8101 gttatcctat attgcaagtc gttgattacg ttttgggttt ccacccgtcg gctcaatcgc
     8161 cgtcaaccct gttatcgtct gtcgtaaaac aacctttaga atttttttca caaacagcca
     8221 ttttttgtta gtcgacgaaa tactcttttc tctgcccctt attcccgcta ttaaaaaaaa
     8281 caattaaacg taaactttgc gcaattcagg ccgataaccc cggtattcgt tttacgtgtc
     8341 gaaagataaa aggaaatcgc atggcaagta tttcatcgct gggagtcggg tcaggtctgg
     8401 atttaagttc aatccttgat agcctcaccg ccgcgcaaaa agcgacgcta acccctattt
     8461 caaatcagca atcgtcgttt accgctaaac ttagcgccta cggtacgctg aaaagcgcgc
     8521 tgacgacttt ccagaccgcc aatactgcat tgtctaaagc cgatcttttt tccgccacca
     8581 gcaccaccag cagcaccacc gcgttcagtg ccaccaccgc gggtaacgcc atcgccggga
     8641 aatacaccat cagcgtcacc catctggcgc aggcgcaaac cctgaccacg cgcaccacca
     8701 gagatgatac gaaaacggcg atcgccacca gcgacagcaa actcaccatt caacaaggcg
     8761 acgacaaaga tccgattacc attgatatca gcgcggctaa ctcatcgtta agcgggatcc
     8821 gtgatgccat caacaacgca aaagcgggcg taagtgcgag catcattaac gtgggtaacg
     8881 gtgaatatcg tctgtcagtc acatcaaatg acaccggcct tgataatgcg atgacactct
     8941 cggtcagcgg tgatgatgcg ctacaaagtt ttatgggcta tgacgccagt gccagcagca
     9001 acggtatgga ggtctcggtc gccgcccaga atgcgcagct gacggtcaac aacgtcgcca
     9061 tcgagaacag cagcaacact atcagcgacg cgctggaaaa catcactctg aacctgaacg
     9121 atgtcaccac gggcaaccag acgctaacca tcactcagga cacctccaaa gtgcaaacgg
     9181 cgattaaaga ctgggtgaat gcctataact cgctaataga taccttcagc agcctgacca
     9241 aatacaccgc cgtagatgcg ggagctgata gccagagttc tagcaatggc gcactgctcg
     9301 gcgactccac gctgcggacg attcagacgc agttgaagtc gatgctgagt aataccgtca
     9361 gttcttccag ctataaaacg ctggcgcaga ttggtatcac gaccgatccc agcgatggca
     9421 aactggaact ggatgccgac aaactcaccg ctgcactgaa aaaagatgcc agcggcgtag
     9481 gtgcattgat tgttggcgat ggtaaaaaaa ccggcatcac aaccaccatc ggcagcaacc
     9541 tgaccagttg gctttcgaca acgggcatta ttaaagccgc taccgatggc gttagtaaaa
     9601 ccctgaataa gttaactaaa gactacaacg ccgccagcga tcgcattgat gcgcaggtcg
     9661 cgcgctacaa agaacaattt acccaactgg acgttttaat gacctcgtta aacagcacca
     9721 gcagctactt aacgcagcag ttcgaaaaca acagtaattc caagtaatca ttattcatcg
     9781 ggagacaggt catgtacgcg gcaaaaggca cccaggccta tgcacaaatt ggcgtcgaaa
     9841 gcaccgtaat gagcgccagc caacagcagt tggtcaccat gctatttgat ggagtgctga
     9901 gcgcactggt tagagcgcga ctgtttatgc aggacaacaa tcagcaaggc aaaggcgtct
     9961 ctttgtcaaa agcgatcaac atcattgaga acggactgcg ggtgagtctt gatgaagaga
    10021 gcaaagacga attaacccaa aacttaattg ctctttatag ctatatggtc aggcgtttgc
    10081 tgcaagccaa tttacgcaac gatgtctcag cagtcgaaga ggtggaagca ttaatgcgta
    10141 atattgccga tgcctggaaa gagtctttac tctccccttc ttcgattcag gacccagtct
    10201 gatgaacaat gcaccgcatt tgtatttcgc ctggcaacaa ctcgtcgaaa aaagccagct
    10261 catgttacgc ctggcaacgg aagaacaatg ggacgaactc atcgctagcg aaatggcgta
    10321 tgtgaatgcg gtgcaggaga tcgcacacct gactgaagag gttgccccgt ccactacgat
    10381 gcaggagcag ctccgcccga tgctgcacct gattctcgac aacgaaagca aggtaaagca
    10441 gttattacag attcggatgg atgaactggc gaaactggtc ggtcagtcat cggtgcaaaa
    10501 atcggtgtta agtgcctatg gcgatcaggg cggctttgtg ctggctccgc aggataacct
    10561 cttttgaatc ttaatgagcc gatggctcgc gaataatccg attacggcta cgcttctaat
    10621 attccccttg aatggagtcg aagaatgcgt aatcccacgc tgttacaatg ttttcactgg
    10681 tattacccgg aaggcggtaa gctctggcct gaactggccg agcgcgcaga cggttttaat
    10741 gatattggta tcaatatggt ctggttgccg cccgcctata aaggcgcatc gggcggctat
    10801 tcggtcggct acgactccta tgatttattt gatctaggtg agtttgatca gaaaggcagc
    10861 atccctacta aatatggtga taaagcacaa ctgctggccg ccattgacgc cctgaaacgt
    10921 aatgacattg cggtgctgct ggacgtggta gtcaaccaca aaatgggcgc ggatgaaaaa
    10981 gaagctattc gcgtgcagcg tgtaaatgct gatgaccgta cgcaaattga cgaagaaatc
    11041 attgagtgtg aaggctggac gcgttacacc ttccccgctc gtgccgggca atactcgcag
    11101 tttatctggg atttcaaatg ttttagcggt atcgaccata tcgaacaccc tgatgaagat
    11161 ggcattttta aaattgttaa cgactatacc ggagaaggct ggaacgatca ggttgatgat
    11221 gaactcggta atttcgatta tctgatgggt gagaatatcg attttcgcaa tcatgccgtg
    11281 acggaagaga ttaaatactg ggcgcgctgg gtgatggaac aaacgcaatg cgacggcttt
    11341 cgtcttgatg cggtcaaaca tattccggca tggttttata aagagtggat cgaacacgta
    11401 caggaagttg cgccaaagcc gctgtttatt gtggctgaat gctggtcgca tgaagtcgat
    11461 aaactgcaaa cgtatattga tcaggtggaa ggcaaaacca tgctgtttga tgcgccgctg
    11521 cagatgaaat tccatgaagc atcgcgcatg ggtcgcgact acgacatgac gcagatcttc
    11581 actgggacgc tggtggaagc cgatcctttt cacgccgtaa ccctcgttgc caaccacgac
    11641 acccaaccgt tgcaagccct cgaagcaccg gtcgaaccgt ggtttaaacc gctggcgtat
    11701 gccttaattt tgttgcggga aaatggcgtt ccttcggtgt tctatccgga cctctacggt
    11761 gcacattacg aagatgtcgg tggtgacggg caaacctatc cgatagatat gccaataatc
    11821 gaacagcttg atgagttaat tctcgcccgt cagcgtttcg cccacggtgt acagacatta
    11881 tttttcgacc atccgaactg cattgccttt agccgcagtg gcaccgacga atatcccggc
    11941 tgcgtggtgg tcatgtcgaa cggagatgat ggcgaaaaaa ccattcatct gggagagaat
    12001 tacggcaata aaacctggcg tgattttctg gggaaccggc aagagagcgt agtgacagac
    12061 gaaaacggcg aagcgacctt cttttgcaac ggcggcagcg tcagcgtgtg ggttatcgaa
    12121 gaggtgattt aaatccatcc ccggcggcaa gccggggaga tttcattacg gcagtggcgt
    12181 cggtaacggc gctttatcca gcgcggcggc acactctgcc gtcgggcgat ccacacgctc
    12241 catcgtcata ccatcgtact caatggtgtt accgtcacgc tcaacctcgt acagctcacg
    12301 ttttacggta acgttagtga gatcatcaga catcaatgtc aacttacccg gcacggcaat
    12361 cccccgctgc cactgacggc aatccaaggt atcgccctct ttagtgacaa tcaggctgcc
    12421 tatcgcttcc ggactgacca gcgcacgttg cggccctttg gtctgccagt agcccgccag
    12481 ccagtccggc gcgggggttt tcacgacatt gttatagttt tcgacttcgg cacagcccgc
    12541 cagcaacatg agcgcgcctg caattgcgag ttttttcatc attcgtcctg catgagaaga
    12601 aaagaggatt gtggcattaa agctctgatg tcgccagcct ttccaggttc agagagaaat
    12661 ttgatctaac tattattttt gtgaattatt accccgaaaa taaatttgca tggtgtatga
    12721 ttcgcggctc ttgtttcaaa ccttctgagt tcagaggcta cattcatgtc atggcagcaa
    12781 ttcaaacacg cctggttgat taaattctgg gcacccatcc ctgcggtcat cgcggcgggt
    12841 attctctcca cttactattt tggcattact ggcacctttt gggctgtcac gggtgaattt
    12901 acccgttggg gcggtcagct cctgcagctg tttggcgtcc atgctgaaga gtggggttat
    12961 tttaaaatta tccatctgga aggatcgcca ttaacccgga tcgacggcat gatgatcctc
    13021 ggtatgtttg gcggctgctt tgctgctgcg ctgtgggcca acaatgtcaa actacgcatg
    13081 ccacgcagcc gtatccgcat tatgcaggcc atcattggcg gcattatcgc cggttttggc
    13141 gcgcgtctgg caatgggctg taacctggcg gcgttcttta ccggcattcc tcagttctcg
    13201 ctgcatgcct ggttctttgc catcgccact gccattggtt catggtttgg cgcgcgcttt
    13261 accctgctgc ccatcttccg tattcccgtg aaaatgcaga aagtttctgc cgcctcaccg
    13321 ctgacgcaaa aaccggatca ggcgcggcgt cgttttcgtc tcgggatgct ggtctttttc
    13381 ggcatgctgg gctgggcgct gctcacggcg atgaaccaac caaaactggg gctggcaatg
    13441 ctgtttggcg tcggctttgg tttactgatt gaacgtgcgc aaatctgctt tacttcggcg
    13501 ttccgcgata tgtggatcac cggacgtacc catatggcga aagcaatcat tatcggtatg
    13561 gcggtgagtg ccatcgggat cttcagttac gtacagttag gcgttgaacc taaaatcatg
    13621 tgggcgggac caaatgcggt aattggtggt ttactttttg gttttggcat cgtgctggca
    13681 ggcggctgcg aaaccggctg gatgtaccgc gcggtagaag gccaggtgca ctactggtgg
    13741 gtcggtctgg gcaacgtgat cggctcaacg attctggcgt attactggga tgatttcgct
    13801 ccggcgctgg ccaccgactg ggacaaaatc aacctgctga aaacttttgg tccgatggga
    13861 ggcctgctgg tgacatattt gctgctgttt gctgcgctga tgttgattat cggctgggaa
    13921 aaacgcttct tccgccgtga ggcaccgcag actgctaagg agatcgcatg aaaaatatcg
    13981 ttcctgatta ccgccttgat atggtgggtg aaccctgccc ttatccggca gtcgcaaccc
    14041 ttgaggcgat gccgcaatta aaaaaaggtg agatcctgga agtggtgagc gactgtccgc
    14101 agtcgatcaa taatattcca ctggatgcgc gtaatcacgg ctacacggtg ctggatattc
    14161 agcaagacgg gccgaccatt cgttatttaa ttcagaagta atccctcatt cccgctgtgg
    14221 cccacagcgg gaacatcgtt acctctgaca catagatttt acccgccccc tctcctattc
    14281 ttttcagtat gatctcagga ggttgctatg tgtggacgct ttgcccaatc ccaaacgcgt
    14341 gaggattacc ttgcgcttct cgcggaagat attgaacgcg atattcccta cgatcccgaa
    14401 cccattggca gatacaatgt cgcgccggga accaaagttc ttctgctcag tgaacgtgat
    14461 gaacaccttc atctggatcc ggttttctgg ggatatgctc ccggatggtg ggataaaccg
    14521 ccgctgatta acgcccgcgt agaaactgcc tcaaccagtc gcatgtttaa accgctctgg
    14581 caacatggtc gggcaatctg ttttgccgat ggctggtttg agtggaaaaa agaaggcgac
    14641 aaaaaaagcc ttattttatc tatcgcgctg acggacaacc tgtttttatg gccgcgatag
    14701 gtagcatacc gtttgagcgt ggtgacgaag ccgaaggatt tttgatcgtc actgcggcgg
    14761 cagatcaagg tctggtagat attcatgacc gccgcccgct ggttctgtcg ccagaagctg
    14821 cacgggaatg gatgcggcaa gacattggtg gtaaagaagc ctcagaaatc gctaccagaa
    14881 gctgtgtgcc agcaaaccag ttcacctggc accctgtgtc gcgcacggtg ggtaatgtca
    14941 aaaaccaggg cgcggagtta attcaacctg tttgttgaca tcctccacgc cctgaatgac
    15001 gaggacccct gctacgttca ggctgtcgcc tgaatcattt cggtgggttc ctgcttcaac
    15061 gagcggcctg actgcaccat ctctccacag gcaagaacgg cgtgccccgc cgctaaaatg
    15121 ttacgagctc catttacatc ggcgttcgct gtatatccac atacctggca tctgaattta
    15181 ctttgtgaca ggcgattttc tttcgctgta tgaccacagc acgcgcaacg ctggcttgtg
    15241 tacgctggcg gaacagcaag cacctgaccg ccacgccaga gctgcttgta ctcaagctgg
    15301 cggcgcattt cataccagcc ctgatccagt atcgaacggt ttaaacctga ttttgcccgg
    15361 acattgcgcc ccggctggct gaccgtaccc gctgctgact ttgacatgtt gctgaccttc
    15421 aaatcctcaa tgactatcat tacgtggttt ttgctgacgg tcgttgtgac tttgtgaagg
    15481 tagtccctgc ggatatttgc gatacaggaa tgcagtcgct gtattttgcg tttctgcttc
    15541 tgccagttgt tgctgaattt gaccttgcgg cttaactgtc gctgaagtct cgccagcgtc
    15601 ttctggtttt tctgaaaact gtttacaggc tcaaagactg tgccatctga cagcgaggca
    15661 agtttagcca cgccagcatc cagcccgacc attgatgctg aagggtgaac cggagttgat
    15721 acttcacttt ctgcctgaat actgatgtac cacttaccgc aggactggct gacagtgaca
    15781 tttttcacaa cacccgtgac ctgacggctg ttccggtagc gcatccagcc aagttttggc
    15841 agaaaaatac ggctgttttc ctgagcgagc ttaacgccct gcggatagcg gaatgcatca
    15901 ttctgtcacc gctttttgaa tcaggggaac gccgagcgct tctggaaaaa gtttttgcag
    15961 gcctgctcag ggtctttcag tgactgttgc aatggctgtg acggagcatc tttaatccat
    16021 tgcgtttcag tggcattttt ccactcaacc aatcaggaag ccattttcgc gtaagggatg
    16081 tctttgttac cggccttatg attctcattc tgacgtgcca gagtacgact gaaaacgaaa
    16141 cgacatgcgc ctgcgaagcg cctcatttca cgcgcctgtt gaccaccggg tcttaaccgg
    16201 aatttgaata cctgtagtcg tttcacaggg caaattatac tttggtctgt gaaaaaaact
    16261 gatatccgac gcggaaggca ctgtgttttc ctgatgcatg ctcaccgggt ctttgttacc
    16321 aagtaccgac gccagatttt tgaccatgac gcgacagaaa aactacgcac ttacttttca
    16381 aatgtatgtg cttattttga agctgaactg gttgaaatgg atggcgaacc agatcacgtc
    16441 catctgttaa ttaactatcc ccccaaactg gcgatatcca gtctggtaaa caaacagcct
    16501 caaaggcgta tcgggtaggt tactgcgacg ggatcgacca gatattgctg ccaggtatta
    16561 ccgcaaagat gttttgtggc gtcccggcta ttttgccagt agctgcggag gtgcgccaat
    16621 atccgtcatc cgccaataca ttgaacaaca gcaaacacct ggtcagatga aaaaacgagc
    16681 cttatatccc cgccctgaag gacgggcttt tacggtgcac tggataagcc tggagattat
    16741 ctacacctgc atggacatca ttgtactatg ccccgtacat gctccatcac atcctcaaca
    16801 tcaaagtatt agagccttgc aaaccattat gcgtctcagt ttgtctcatc tatcttacgc
    16861 aaaaaaaata gaactaatat caagcggcgc tatattgcgt attaaataaa accacctcaa
    16921 ttacattaat aaatctaaat aaatatcatg caaataattt ccatggttat ttttaggcta
    16981 tttaaattaa aaaaataaat tatgagtcaa tcataaataa caacctttaa tataatttga
    17041 ctaccaaatt tatattcgtt cacaaaatag tcatgcattg tgtgcaatag aaacagttca
    17101 gataaaaata gggatctact tcacaattta aatgagaaac taaaacttac atcttgaaat
    17161 aatctcattg attggatgaa tatttgtagt gcagtgacat catttttaaa taatagttca
    17221 aataaaaggg ctcacgatga aaaaattaac agtggcaatt tctgctgtag ctgcatcagt
    17281 actgatggcg atgtctgctc aggcagcaga gatttacaat aaagacagta acaagctgga
    17341 tctgtacgga aaagttaatg ctaagcacta cttctcttct aacgatgcag atgatggcga
    17401 cacaacttat gttcgtctgg gcttcaaagg cgaaactcaa atcaacgatc aactgactgg
    17461 tttcggtcag tgggaatatg aattcaaagg caaccgtgct gaatctcaag gttcttccaa
    17521 agataaaacc cgtcttgcct tcgctggcct gaaattcggt gactacggct ccatcgatta
    17581 tggccgtaac tacggtgtag catacgatat cggtgcatgg accgacgttc tgccagaatt
    17641 cggtggcgat acctggaccc aaacagatgt gttcatgact ggtcgcacca caggtgttgc
    17701 aacctatcgt aacaacgact tctttggtct ggttgatggt ctgaactttg ctgctcagta
    17761 tcagggtaaa aatgaccgca ctgacgtaac tgaagctaac ggtgatggtt tcggtttctc
    17821 cactacttat gagtatgaag gattcggtgt aggtgcaacc tatgctaaat ctgaccgcac
    17881 taatgatcag gttatctacg gtaacaacag cctgaatgca tctggtcaaa atgctgaagt
    17941 atgggcagct ggtctgaaat atgatgcgaa caacatctat ctggctacca cctattctga
    18001 aacccagaac atgactgttt ttggtaataa ccatattgcc aacaaagcac aaaacttcga
    18061 agtagttgca caatatcagt tcgacttcgg tctgcgtccg tccgttgctt acctgcaatc
    18121 taaaggaaaa gacttgggtg cgtggggtga tcaggacctg attgaatata ttgatgtagg
    18181 tgcaacctat tacttcaaca aaaatatgtc cacttttgtt gattacaaaa tcaacctgat
    18241 tgataagagc gatttcacga aagcatctgg cgttgctacc gatgatatcg ttgctgtagg
    18301 attggtttac cagttctaat ctgattactc aaagatatgt tgtgggaggc tttgcctccc
    18361 caacatataa gtggcgctct caagccactt tccttagaag cactatcttg cttctgacta
    18421 tataaattac cctttatttt ctgggacgtt tcaacgcccc atttttaata atactcagtg
    18481 aacaattaac gtatcaatta gatttaataa caacgatatc cgtacctaac ctgatatcta
    18541 atgtcataag catcccttta attatcccct caacctcttg taaatttccc actcttgagc
    18601 tccacatagc ccaaacgccg gatgcaaaaa aaatcatacg tgcatctctg aaattgtgat
    18661 ggaggaagtg agtatcaatt atatgtgctt ataaacgttc ccgattggca cagaagtcac
    18721 cggattgttc aggctccaat aggttgagta tgtcgtattt attttccaaa ataaaaatcg
    18781 atgctgttgc tccttaataa aatactttta ctctctggac gcttttctta gctctcttgg
    18841 tgttatatta aaacgattat ggaacctttc agtaaaacga gaaggacact tgtaaccatt
    18901 ttccctggca atctcgctga tgggttttac tgtcgtttgt atagcaggac aacgcattat
    18961 ttaacctcac gtcgtccaat atactttgaa aacttattcc ctcgcttgcc agacggcgat
    19021 gcaatgtgga aacagaaatg tagagatatc tcgctacctt gtttgctgtc cattttgttc
    19081 taggttcgga tagcattagg ttataacaac gacttattaa tgattgttta ctatctgata
    19141 aaagtaaaca attaacatga tgtgctccta atgaaagtag aacgcccatt gctaagtgtt
    19201 cctgaatttg cgttgagaag cctcgggaaa tagatgtttt tagttgctcc caacaatata
    19261 ttaactcggg gttctgaggt aagcagaaac ctgtcgtgtt acgaatttga tcagttatcg
    19321 cataaaattt ttgaaacttt tcaattagat caatgggata ataaagcatt tctgcaagat
    19381 aaagacctgc ttctggataa ttcacaatat aaaattcata tccacaggga aataatatta
    19441 tctgattatt atcaacagtt agactatgcg tctcccaatt gataactttt tttccctgac
    19501 ggatacgaca taaagctggc ataagaggct taaccctgtg aatctcatga tgtttatgca
    19561 tccgtatttc ttcaatcttt aggttagact tatctcttgc cagcatactc ttaccctatt
    19621 ttatctcata aactggtgtt atctcagcgg ttgcaatttt attggcatta agcatataac
    19681 caactaacgc tccgctggaa ttagaatcca caggaatctt atcagttttt agagcccata
    19741 ctttaaactg gtaatgatgg ggtttatctc ctttgggagg acatgctcca ccaaatcctg
    19801 cataaccaaa atcatttcgg ccttgaacag caccagtcgg aagttttgta ccatcacgtc
    19861 ttcctgcatc agtgggcaaa tatgttacag ttgctggaat attagcaaca gtccagtgcc
    19921 accatccact gcctgtagga gcatctggat catacacagt tacggcaaag cttttggtgc
    19981 cttcaggagc accagaccag gttaatgagg gcgatgtatt accaccttca cacccaaatc
    20041 cagaaaatac atgagacgtt gtaagttgct ctcctgtttt tatttcatta ctagtgacct
    20101 gaaatgctgc ggcctgcgca gaaaatgtta tgaatgccaa tacacttgaa acgataagtt
    20161 ttttcataaa aacctcattg ttgtgaccta tcgttatttt atttgatgtg ccttgatctc
    20221 attatgcata aaggcgcaat gatcgcgcaa atgcaatcat aattataaag gtgccccgaa
    20281 cattagccat aatatgcaca aatcagattc ctgttatctc aaggagaatt attttttaat
    20341 gcggtgcact tcgtgacaac aagttaatga ctaacacacc ggcacaaatc aacatcatgc
    20401 ctatgatagc tggcaggtcc agtcgttgac caaaaaatcc ccaggaaagc aaactaatca
    20461 gaacgatacc gactcctgac cagatagcat aagcaatccc tgtaggtata taagccagcg
    20521 tctgagctaa taaccagaat gatgcacaat agcaaataat tgtgccaaca gagggccata
    20581 accgtgtaaa cccctctgaa aacttcatta aggttgtacc aatgacctct gcaagtattg
    20641 cgccaccaag ataaatataa gggttcatag catattcttt cctgttcata ctggagagaa
    20701 ttgtactaca gtttgaacac aactcaccgt tttcatcctg gtgtatgtgt atccaagggg
    20761 ctctggcgtg gcaagagata aaggcaggaa caataatgat cagaaggaac cccaggctaa
    20821 cgaatgagtt cactcttcct gggagcaaag cgaagacaaa acatgttatc gcgtttacgg
    20881 atcgtggatg gcagaaaaca accaggatca ggtatccatt ttaaaccaga aattgagtga
    20941 gtttgcccca tccatgcccc acgctgtggg atcggatata attaaacaag catagtcaac
    21001 aggttacaca acctacacct gcatggacat cacttcctga tacgccgcca ccagcttatt
    21061 acgcacctga atccccattt gcatagaaac tgaggctttt tgcatatcgg tcatcacatc
    21121 gtttagcgcc acgccgggtt caccgagggt gaatttttct gcctgcgtgc gggcgactgt
    21181 ttgtgtatcg cttatgcgat cgagcgcggc gtgcagctgc ccggcaaaac taatggtcgg
    21241 ttgcggcagt gattcctgcg cacgcgcact catcgccgta gcctgtaact ggctgataac
    21301 cccttcaatc ccctgtatcg ctgacatttt cgtctcccgg ataatttctg gtaacaaagc
    21361 ctaccagtaa gtcaataaga caaaggcgct aaatagcaac aaaaaaactg gtttattggc
    21421 ggatagaaaa aacgaaagca caaataatgg gagcgtcaat ttttcgagtg tgctgacccg
    21481 agagtgagtc ttgttccact ttgccaataa cgccgtccat aatcagccac gaagtgcgcg
    21541 atgaatgcga ctgcagccca gacaaaatct cttgagtggc ttaatcgcct gcgtgcgaat
    21601 ccgaaaattc cattgattgt taccggttcc gcggcagtgg cggtcatggt cgcactgatc
    21661 ctgtgggcga aagcccccga ctaccgcaca ttattcagca atctttccga tcaggatggt
    21721 ggcgcaattg tcagccaact gacgcaaatg aatattcctt accgcttcag cgaagccagc
    21781 ggcgctattg aggttccggc agataaagtt cacgaactgc gcctggcgca acaaggtttg
    21841 cccaaaggcg gcgcggtcgg tttcgaactg ctcgatcagg aaaagtttgg tatcagccag
    21901 ttcagcgaac aggtgaatta tcagcgggcg ctggaaggcg agctttctcg taccatcgaa
    21961 actatcggcc cggtaaaagg ggcgcgcgta catctggcaa tgccgaaacc gtctttattc
    22021 gtccgtgaac aaaaatcccc ttctgcatcg gtgacggtaa atctgttacc cggccgcgca
    22081 ctcgatgaag ggcaaattag cgctattgta catctggttt ccagcgcggt tgctggtctg
    22141 ccgcctggaa acgtcacgct ggtggatcag ggcgggcatc tgttaaccca gtccaatacc
    22201 agcgggcgcg atcttaatga cgctcagttg aaatatgcca gcgatgtcga aggccgtatt
    22261 cagcagcgta ttgaagcgat cctgtcacct attgttggta acggtaatat tcacgcctag
    22321 gtcacggcgc aactggactt cgccagtaaa gaacaaacgg aagaacagta tcgccctaac
    22381 ggtgatgaat ctcatgcggc gcttcgttca cgccagctta atgagagcga gcaaagcggt
    22441 tccggttatc cgggcggcgt accgggggcg ttgtcgaatc aaccggcacc tgcgaataac
    22501 gcgccaatca gcacgcctcc gacaaatcaa aataaccgcc agcagcaggc gagcaccacc
    22561 agcaatagtg ggccgcgtag cacacagcgg aatgaaacca gtaactacga agtcgatcgc
    22621 accattcgtc ataccaaaat gaacgtgggc gatgtgcaac gtctgtcagt cgcggtagtg
    22681 gtgaattaca aaaccttgcc agacggtaaa ccattgcctc tcagcaacga acagatgaag
    22741 caaattgaag ctctgacccg cgaggcgatg ggcttttctg aaaaacgcgg tgattcgctc
    22801 aatgttgtta actcgccgtt caatagcagt gacgaaagcg gcggagcact gccgttttgg
    22861 caacagcaag tgtttatcga tcagttgctg gctgccggtc gctggttgct ggttgctggt
    22921 actgctggtg gcgtggttgc tgtggcggaa agcggtacgc ccgcagctaa cacgtcgcgc
    22981 cgaggcggtg aagactgtgc agcaacaggc gcaggcccgc gaggaagtgg aagatgcggt
    23041 ggaagtccgt ctgagcaaag acgaacaact ccaacaacgg cgcgctaacc aacgactggg
    23101 ggcagaagtc atgagccagc gtatccgtga aatgtctgat aacgatccgc gcgtggtggc
    23161 gctggtcatt cgccagtgga taaataacga tcatgagtaa cctgacaggc accgataaaa
    23221 gcgtcatcct gctgatgacc attggcgaag accgggcggc agaggtgttc aagcacctct
    23281 cccagcgcga agtgcaaacc ctgagcgctg caatggcgaa cgtcacgcag atctccaaca
    23341 agcagctaac cgatgtgctg gcggagtttg agcaagaagc tgaacagttt gctgcactga
    23401 atatcaacgc caacgattat ctgcgttcgg tattggtcaa agctctgggt gaagaacgtg
    23461 ccgccagcct gctggaagat attctcgaaa cccgcgatac cgccagcggt attgaaacgc
    23521 tcaactttat ggagccgcag agcgccgccg atctgattcg cgatgagcat ccgcaaatta
    23581 tcgccaccat tctggtgcat ctgaggcgcg cccaggccgc cgatattctg gcgttgttcg
    23641 atgaacgtct gcgccacgac gtgatgttgc gtatcgccac ctttggcggc gtgcagccag
    23701 ccgcgctggc cgaactgacc gaagtactga atggcttgct cgacggtcag aatctcaagc
    23761 gcagcaaaat gggcggcgtg agaacggcag ccgaaattat caacctgatg aaaacgcagc
    23821 aggaagaagc cgttattacc gccgtgcgtg aattcgacgg cgagctggcg cagaaaatca
    23881 tcgacgagat gttcctgttc gagaatctgg tggatgtcga tgatcgcagc attcagcgtc
    23941 tgttgcagga agtggattcc gaatcgctgc tgatcgcgct gaaaggggcc gagcagccac
    24001 tgcgcgagaa attcctgcgc aatatgtcgc agcgtgccgc cgatattctg cgcgacgatc
    24061 tcgccaagcg tggtccggtg cgtctgtcgc aggtcgaaaa cgaacagaaa gcgattctgc
    24121 tgattgtgcg ccgtcttgcc gaaactggcg agatggtgat tggcagcggc gaggatacct
    24181 atgtctgata atctgccgtg gaaaacctgg acgccggacg atctcgcgcc accaccggca
    24241 gagtttgtgc caatggtcga gtcggaagaa accattattg aagaggccga acccagcctt
    24301 gagcagcaac tggcgcaact gcaaatgcag gcccacgagc agggttatca ggcggggatt
    24361 gccgaaggtc gccagcaagg tcacgaacag ggctatcagg aaggactggc ccaggggctg
    24421 gaacaaggtc tggcagaggc gaaggctcaa caagcgccaa ttcatgcccg gatgcagcaa
    24481 ctggtcagcg aatttcaaac tacccttgat gcgcttgata gtgtgatagc ttcgcgcctg
    24541 atgcagatgg cgctggaggc agcacgtcag gtgatcggtc agacaccaac ggtggataac
    24601 tcggcactga tcaaacagat ccaacagttg ttgcagcaag aaccgttatt cagcgggaaa
    24661 ccacagctgc gcgtacaccc ggatgatctg caacgtgtgg atgatatgct cggcgcaact
    24721 ttaagtttgc atggctggcg cttgcggggc gatcccaccc tccaccctgg cggatgtaaa
    24781 gtctccgccg atgaaggcga tctcgacgcc agtgtcgcca ctcgctggca agaactctgc
    24841 cgtctggcag caccaggagt ggtgtaatga ccacgcgcct gactcgctgg ctaaccacgc
    24901 tggataactt tgaagccaaa atggcgcagt tgcctgcggt acgtcgctac gggcgattaa
    24961 cccgcgctac cgggctggtg ctggaagcca ccggattaca attgccgctc ggcgcaacct
    25021 gtgtcattga gcgccagaac ggcagcgaaa cgcacgaagt agaaagcgaa gtcgttggct
    25081 ttaacggtca acggctgttt ttaatgccgc tggaggaagt ggaaggtgtc ctgcccggcg
    25141 cgcgtgtata tgccaaaaac atttcggcag aagggctgca aagcggcaag cagttgccgc
    25201 tcggtccggc gttattaggt cgtgttctgg acggtagcgg taaaccgctc gatggcctgc
    25261 ccttccccga tacgacggaa accggtgcgc tgattacccc gccatttaac ccgttgcaac
    25321 gtacaccgat tgaacatgtg ctggacaccg gcgtgcgccc aatcaatgcc ctgcttaccg
    25381 tcggacgcgg gcagcgtatg gggctgtttg ccgggtcggg cgttggtaaa agtgtgctgc
    25441 tggggatgat ggcacgctac acccgcgccg atgtcattgt cgtgggtttg attggtgaac
    25501 gtgggcgcga agtaaaagat tttattgaga atatcctcgg tgccgaagga cgtgcacgct
    25561 cagtggtgat tgccgctccg gcggatgttt ctccgctcct gcgaatgcaa ggtgccgcct
    25621 atgccacgcg cattgccgaa gattttcgcg atcgtggtca gcatgtgttg ctgattatgg
    25681 actccctcac ccgctacgcg atggcccagc gtgaaattgc gctggcgatt ggcgaaccac
    25741 ctgccactaa aggttatcca ccgtcggtgt ttgccaaatt accggcactg gtcgagcgtg
    25801 ccggtaatgg cattagcggc ggcggctcga ttaccgcgtt ttataccgtg ctcaccgaag
    25861 gcgatgacca gcaggacccc attgctgact ccgcgcgggc aatcctcgac ggtcacattg
    25921 tgctgtctcg ccgactggcg gaagccgggc actatccggc tatcgatatt gaagcgtcga
    25981 tcagtcgcgc aatgacggcg ttgatcagtg agcaacatta cgcgcgagtg cgcaccttca
    26041 aacagctgtt gtcgagtttt cagcgtaacc gcgatctggt tagcgtcggc gcgtatgcca
    26101 aaggcagcga tccgatgctc gataaagcca tcgccctgtg gccgcagctg gagggctatt
    26161 tgcaacaagg catttttgaa cgcgcggact gggaagcgtc tctccagggg ctggagcgta
    26221 ttttcccgac agtgtcataa cccaggagat aacggcagat ggcggaagaa cagctcaaaa
    26281 tgttgatcga ttatcagaat gaatatcgca ataacctcaa tagcgatatg agtgccggga
    26341 tgaccagcaa ccgctggatc aactatcagc agtttatcca gacgctggaa aaagccatta
    26401 ctcagcatcg ccagcaactt aatcagtgga cgcagaaagt tgacattgcc ctgaacagct
    26461 ggcgagaaaa gaaacaacgt ttgcaggcct ggcagacact gcaggaacgg caatccacgg
    26521 cggcactgct tgcagaaaac cgcctcgatc agaaaaagat ggatgagttc gcccagcgcg
    26581 ccgccatgag gaaacctgaa tgattcgctt agcgcccttg attaccgccg acgttgacac
    26641 caccacattg cctggcggca aagccagcga tgctgcacaa gattttctca cgttgttgag
    26701 cgaagcatta gcaggcgaga caacaactga caaagcggcc ccccagttgc tggtagcaac
    26761 agataagccc acgacaaaag gcgagccgct ggtcagcgat attcttgccg atgcgcaaca
    26821 agcggattta ctgatccctg tggatgaaac acttcctgtc atcaacgacg aacaatccac
    26881 atcaacaccg ttaaccaccg ctcagacgat gacgttggct gcggtggctg acaaaaatac
    26941 gacaaaagac gaaaaagcgg atgatcttaa tgaagacgtc accgcaagtc tgagcgccct
    27001 ttttgcgatg ttgccgggtt ttgacaatac gcccaaagtg actgatgcac cgtcaaccgt
    27061 gttaccggca gagaaaccaa cgctcttcac aaaactgact tctgcgcaac tcactacagc
    27121 acagcctgat gacgcccccg gcacaccagc tcagccatta acaccgctgg tagcagaagc
    27181 ccagagtaaa gcggaagtca tcagcacgcc ttcgccggtg accgctgacg ccagcccgct
    27241 aatcactcca caccagacac agccactgcc caccgtcgcc gcgcctgttt tgagtgcacc
    27301 gctgggttct cacgaatggc aacaatcatt gagccagcat atttcgctgt tcacccgcca
    27361 ggggcaacaa agtgcagagt tgcgtctgca cccacaggat ttaggtgaag tgcaaatttc
    27421 cctcaaagtg gatgataacc aggcgcagat ccagatgatt tcaccgcatc agcacgtacg
    27481 cgccgccctg gaagcagcgc tgccggtact gcgtacgcag ctggccgaaa gtggcattca
    27541 gttagggcaa agcaacatta gtggcgaaag ctttagtggt cagcagcagg ccgcttccca
    27601 gcaacagcaa agccaacgca cagcaaacca tgaacctctg gcgggggaag acgacgatac
    27661 gcttccggtt cccgtctctt tacaagggcg tgtaacaggt aacagcggcg ttgatatttt
    27721 cgcctaacgt cagaggtagc accgtaatcc gcgtcttttc cccgctttgt tgcgctcaag
    27781 acgcaggata attagccgat aagcagtagc gacacaggaa gaccgcaaca catgactgat
    27841 tacgcgataa gcaagaaaag caagcgatcg ctttggatcc cgattctggt attcattacc
    27901 ctcgcggcct gtgccagcgc aggttacagc tactggcatt cgcatcaggt tgccgctgac
    27961 gacaaagcgc agcaacgcgt cgtgccctcc ccggtcttct atgcgctgga tacctttacg
    28021 gtcaatttgg gcgatgcgga tcgcgtgctt tatatcggca taaccctgcg actgaaagat
    28081 gaagcaaccc gctcgcggct gagtgagtat ttgccggaag tccgtagtcg cttgctgtta
    28141 ctgttttcac gtcaggatgc tgccgtactg gcgacagaag aaggcaagaa aaacctgatt
    28201 gccgagatta aaaccacact ttccaccccg cttgttgccg ggcaaccgaa acaggatgtc
    28261 accgacgtgc tgtataccgc ttttattctg cgataacgac atgggcgata gtattctttc
    28321 tcaagctgaa attgatgcgc tgttgaatgg tgacagcgaa gtcaaagacg aaccgacagc
    28381 cagtattagc ggcgaaagtg acattcgtcc gtacgatccg aatacccaac gacgggttgt
    28441 gcgcgaacgt ttgcaggcgc tggaaatcat taatgagcgc tttgcccgcc attttcgtat
    28501 ggggctgttc aacctgctgc gccgtagccc ggacattacc gtcggggcca tccgcattca
    28561 gccgtaccat gaatttgccc gcaacctgcc ggtgccgacc aacctgaacc taatccacct
    28621 gaaaccgcta cgcggcacag ggctggtagt gttctcgccg agtctggtgt ttatcgccgt
    28681 ggataacctg tttggcggcg atggacgctt cccgaccaaa gtggaaggtc gcgagtttac
    28741 ccatactgaa cagcgcgtca tcaaccgtat gttgaagctg gcgctggaag gctatagcga
    28801 cgcctggaaa gcgattaatc cgctggaagt tgagtacgtg cgttcggaaa tgcaggtgga
    28861 atttaccaat atcaccacct cgccgaacga cattgtggtt aacacgccgt tccatgtgga
    28921 gattggcaac ctgaccggcg aatttaatat ctgcctgcca ttcagcatga tcgagccgct
    28981 acgggaattg ttggttaacc cgccgctgga aaactcgcgt aatgaagatc agaactggcg
    29041 cgataacctg gtgcgccagg tgcagcattc acagctggaa ctggtggcca actttgccga
    29101 tatctcgcta cgcctgtcgc agattttaaa actgaaaccc ggcgacgtcc tgccgataga
    29161 aaaacccgat cgcatcatcg cccatgttga cggcgtcccg gttctgacca gtcagtacgg
    29221 tactctcaac ggtcagtatg cgttacggat agaacatttg attaacccga ttttaaattc
    29281 tctgaacgag gaacagccca aatgagtgac atgaataatc cggccgatga caacaacggc
    29341 gcaatggacg atctgtgggc tgaagcgttg agcgagcaaa aatcaaccag cgaaaaaagc
    29401 gctgccgacg cggtgttcca gcaatttggc ggtggtgatg tcagcggaac gttgcaggat
    29461 atcgacctga ttatggatat tccggtcaag ctgaccgtcg agctgggccg tacgcggatg
    29521 accatcaaag agctgttgcg tctgacgcaa gggtcagtcg tggcgctgga cggtctggcg
    29581 ggcgaaccgc tggatattct gatcaacggt tatttaatcg cccagggcga agtggtggtt
    29641 gttgccgata aatatggcgt gcggatcacc gatatcatta ccccgtccga gcggatgcgc
    29701 cgcctgagcc gttagtgatg aataaccatg ctactgtgca atcttccgcg ccggtttctg
    29761 ctgagccact gctgcaggtg agcggcgcac tcatcgccat tattgccctg atcctcgctg
    29821 ctgcctggct ggtaaaacgg ctgggatttg cccctaaacg cactggcgtt aacggtctga
    29881 aaattagcgc cagtgcttcg ctgggcgcgc gtgaacgggt cgtggtggtc gatgtggaag
    29941 atgcacggct ggtgctcggc gttaccgcag
//
LOCUS       DAJTSI010000037        29799 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000037 DAJTSI010000000
VERSION     DAJTSI010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29799
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.037"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            56..850
                     /locus_tag="OO327_001465"
     CDS             56..850
                     /locus_tag="OO327_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR6018056.1"
                     /translation="MKIFKYTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLY
                     DAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETW
                     FNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDI
                     TMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVH
                     HHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            872..2281
                     /locus_tag="OO327_001466"
     CDS             872..2281
                     /locus_tag="OO327_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6018057.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2259..3038
                     /locus_tag="OO327_001467"
     CDS             2259..3038
                     /locus_tag="OO327_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR6018058.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3035..3895
                     /locus_tag="OO327_001468"
     CDS             3035..3895
                     /locus_tag="OO327_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR6018059.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4043..4618)
                     /locus_tag="OO327_001469"
                     /pseudo
     CDS             complement(4043..4618)
                     /locus_tag="OO327_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4635..4895)
                     /locus_tag="OO327_001470"
     CDS             complement(4635..4895)
                     /locus_tag="OO327_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR6018060.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4886..6157)
                     /locus_tag="OO327_001471"
     CDS             complement(4886..6157)
                     /locus_tag="OO327_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6018061.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWNVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6235..6597)
                     /gene="queD"
                     /locus_tag="OO327_001472"
     CDS             complement(6235..6597)
                     /gene="queD"
                     /locus_tag="OO327_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR6018062.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6916..8715
                     /gene="cysJ"
                     /locus_tag="OO327_001473"
     CDS             6916..8715
                     /gene="cysJ"
                     /locus_tag="OO327_001473"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR6018063.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8715..10427
                     /gene="cysI"
                     /locus_tag="OO327_001474"
     CDS             8715..10427
                     /gene="cysI"
                     /locus_tag="OO327_001474"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR6018064.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10501..11235
                     /gene="cysH"
                     /locus_tag="OO327_001475"
     CDS             10501..11235
                     /gene="cysH"
                     /locus_tag="OO327_001475"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR6018065.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11499..11651
                     /locus_tag="OO327_001476"
     CDS             11499..11651
                     /locus_tag="OO327_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR6018066.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11845..12936
                     /locus_tag="OO327_001477"
                     /pseudo
     CDS             11845..12936
                     /locus_tag="OO327_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(13082..14119)
                     /gene="iap"
                     /locus_tag="OO327_001478"
     CDS             complement(13082..14119)
                     /gene="iap"
                     /locus_tag="OO327_001478"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR6018067.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGGCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            14371..15279
                     /gene="cysD"
                     /locus_tag="OO327_001479"
     CDS             14371..15279
                     /gene="cysD"
                     /locus_tag="OO327_001479"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR6018068.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15281..16708
                     /gene="cysN"
                     /locus_tag="OO327_001480"
     CDS             15281..16708
                     /gene="cysN"
                     /locus_tag="OO327_001480"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR6018069.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16708..17313
                     /gene="cysC"
                     /locus_tag="OO327_001481"
     CDS             16708..17313
                     /gene="cysC"
                     /locus_tag="OO327_001481"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR6018070.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            17363..17686
                     /locus_tag="OO327_001482"
     CDS             17363..17686
                     /locus_tag="OO327_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR6018071.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            17880..18191
                     /gene="ftsB"
                     /locus_tag="OO327_001483"
     CDS             17880..18191
                     /gene="ftsB"
                     /locus_tag="OO327_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR6018072.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            18210..18920
                     /gene="ispD"
                     /locus_tag="OO327_001484"
     CDS             18210..18920
                     /gene="ispD"
                     /locus_tag="OO327_001484"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR6018073.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18920..19399
                     /gene="ispF"
                     /locus_tag="OO327_001485"
     CDS             18920..19399
                     /gene="ispF"
                     /locus_tag="OO327_001485"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR6018074.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            19396..20445
                     /gene="truD"
                     /locus_tag="OO327_001486"
     CDS             19396..20445
                     /gene="truD"
                     /locus_tag="OO327_001486"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR6018075.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            20426..21187
                     /gene="surE"
                     /locus_tag="OO327_001487"
     CDS             20426..21187
                     /gene="surE"
                     /locus_tag="OO327_001487"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR6018076.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLAAHSAQDVVSDWLNSVGVGTQW"
     gene            21181..21807
                     /gene="pcm"
                     /locus_tag="OO327_001488"
     CDS             21181..21807
                     /gene="pcm"
                     /locus_tag="OO327_001488"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR6018077.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21947..23086
                     /gene="nlpD"
                     /locus_tag="OO327_001489"
     CDS             21947..23086
                     /gene="nlpD"
                     /locus_tag="OO327_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR6018078.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            23149..24141
                     /gene="rpoS"
                     /locus_tag="OO327_001490"
     CDS             23149..24141
                     /gene="rpoS"
                     /locus_tag="OO327_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR6018079.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(24261..24668)
                     /locus_tag="OO327_001491"
     CDS             complement(24261..24668)
                     /locus_tag="OO327_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR6018080.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24815..25408
                     /locus_tag="OO327_001492"
     CDS             24815..25408
                     /locus_tag="OO327_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR6018081.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            25408..26355
                     /locus_tag="OO327_001493"
                     /pseudo
     CDS             25408..26355
                     /locus_tag="OO327_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26628..27191)
                     /locus_tag="OO327_001494"
     CDS             complement(26628..27191)
                     /locus_tag="OO327_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018082.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            27356..27607
                     /locus_tag="OO327_001495"
     CDS             27356..27607
                     /locus_tag="OO327_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6018083.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            27609..29705
                     /locus_tag="OO327_001496"
     CDS             27609..29705
                     /locus_tag="OO327_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6018084.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         7294 a         7383 c         8064 g         7058 t
ORIGIN      
        1 tcgtcacatc atcatcggca acgaaggtgc attgtgctat atcactgaag taacagtgaa
       61 aatctttaaa tacaccccgg aaaacaacct cttctacggc tatatcctgg aagacatgaa
      121 aaccggcttc aacatcctgc gtgaaatcat ggtggaaggg tatcgtccgt cgatcgctcg
      181 tttgtatgac gctgaagatg gcacccaaca cttcacccat tttgccgacg gaaaatgtgt
      241 gctgatcttt atggctgaag gtaaccctcg cattgcgaag gcgacgggcg aagggattgc
      301 ggaaatcgtt gcccgctacc cgcagtgcca gcgcgtggac agcaaactga ttgaaacctg
      361 gtttaacaac ctgaactggg gaccggataa agtggctgcc gaacgtgtgc agatcctcaa
      421 aaccggcaac atgggcttta ccaccgaagt gtccggctgc tggagctgca tccacgaaat
      481 ctacgaaagc gttattaacc gtattcgtac tgagttcccg cacgccgacg acatcaccat
      541 gctgggcggt cactcctctc atagctatca gaacggcacc aacatgtact tcgtctatga
      601 ctacaacgtt gttgactgta agccggaaga ggaaatcgac aagtaccaca atccgctcaa
      661 caaaatcatc tgcgaagaaa ccattcgcct cggcggttcg atggtgcacc accatggcat
      721 tggtaaacat cgcgttcact ggagcaagct ggaacacggc agcgcgtggg cgttgctgga
      781 agggctgaaa aagcagttcg atcctaacgg cattatgaat acgggtacta tctatccgat
      841 tgaaaaataa tgtatcaggc agcgttccgc gatgacgggg cgttgccttt tcggcttctc
      901 aggcgagaag ccgttcttat taccggacaa tgaaggggta aagatgaaca cttcaccggt
      961 gcgaatggat gatttaccgc ttaaccgttt tcactgtcga attgctacgc tcactttcgg
     1021 cgcacacctg accgacggtt atgttctagg cgtcattggt tacgccatta ttcaacttac
     1081 gcccgccatg caactgacgc cgtttatggc gggaatgatc ggcggctcgg cgctccttgg
     1141 tttgtttctt ggcagcctgg ttcttgggtg gatctccgac catattggtc ggcaaaaaat
     1201 cttcaccttc agctttttgc tgattacgct cgcttcgttc ttgcaatttt ttgccaccac
     1261 gccagagcat cttattgggc tgcgcatttt gatcggcatt ggtctgggag gcgattactc
     1321 agtaggtcac accttgctgg ctgaattttc cccgcgccgc catcgcggta ttttgctggg
     1381 cgcattcagc gtggtgtgga ccgtaggcta tgtgttggca agtattgccg gacatcactt
     1441 tatttccgaa aacccggagg cctggcgctg gttgctggca tcggcagctc tgcccgcgtt
     1501 gttgattacg ttattacgct ggggaacgcc agaatcgcca cgttggctac tgtgccaggg
     1561 gcgttttgca gaagctcacg ctatcgtgca tcgctatttt ggtccccatg ttttactggg
     1621 cgatgaagtg gtaacggcga cccataaaca catcaaaacc ttgttctctt cgcgttactg
     1681 gcggcgcacg gcgtttaaca gcgtcttctt tgtctgcctc gtaatcccat ggtttgtgat
     1741 ttatacctgg ctgccaacta tcgcccagac tattggtctg gaagatgcgc tgactgccag
     1801 cctgatgctt aatgcgttgt taattgtggg cgcgctgctg ggattagttc tgacgcacct
     1861 gctggcacat cgcaaatttt tgctgggaag ttttttgctg ctggcggcaa cgctggtagt
     1921 aatggcctgt ttgccttccg gcagttcatt aacgctgctg ctttttgttc tcttcagcac
     1981 caccatttcg gcagtcagta atctggtggg cattttgcct gcggaaagtt ttcctactga
     2041 cattcgctcg ctgggagtcg gttttgccac cgccatgagt cgacttggcg cggcggtaag
     2101 tactggcctg ctgccgtggg tgctggcgca gtggggaatg caagtcacct tattgctcct
     2161 agcgacagtg ttgttggttg gttttgttgt gacctggcta tgggcaccag aaactaaagc
     2221 cctcccgctg gtggcggcgg gaaatgtagg aggtgcgaat gaacattctg ttagcgttta
     2281 aagccgaacc ggatgccgga atgctggcgg aaaaagagtg gcaggcggcg gctcagggta
     2341 atagcggacc ggatgtttcg ctactgcgaa gtttactcgg tgctgatgaa caggccgctg
     2401 ccgcgctgct gttagcgcag agaaaaaacg gtacgccgat gtctttaacc gcgttgagca
     2461 tgggggatga acgggcgttg cactggctgc gctatctcat ggctcttggg tttgaggaag
     2521 ctgtattgct ggagacggcg gcagatctgc gctttgcgcc ggaatttgtc gctcgccata
     2581 ttgccgaatg gcagcgtcag aatccgctgg atctgataat taccggcagc caaagcagcg
     2641 aagggcagaa tgggcaaacg ccatttttgc tggcggaaat gctgggctgg ccctgcttca
     2701 cccaggtgga gcgtttcact ctcgacgcgc tgtttatcac cctcgaacag cgtactgaac
     2761 atgggttgcg ctgttgccgg gttcgcttgc ctgcggtgat tgccgtgcgt cagtgcggtg
     2821 aagtggcctt gccagtaccg ggaatgcgcc agcgaatggc tgcggggaaa gcagaaatta
     2881 tccgtaaaac tgttgctgcc gaaatgccag cgatgcagtg tctgcaactg gcaagagcag
     2941 agcaacgacg cggagcgacg ctgattgatg ggcaaacggt agcagaaaaa gctcaaaagc
     3001 tatggcagga ctatttacgc caaaggatgc agccatgaat atcgcaattg tgacaataaa
     3061 tcaggagaat gccgcaatag cgagctggct ggcggcgcag gatttcagcg gttgtacgct
     3121 ggcgcactgg caaatagaac ctcagcccgt agtggcggaa caggtgctgg atgcgatggt
     3181 ggaacagtgg cagcgaacgc ctgcggacgt tgttctcttt ccgccgggaa cgtttggcga
     3241 tgaactgtcg acccggctgg cctggcgttt acatggtgca agcatctgtc aggtgacttc
     3301 gttagatatt ccaaccgtaa gcatgcgcaa atcccactgg ggaaatgcgt taacggcgac
     3361 gctgcaaaca gaaaaacgcc cgttatgcct ttccctggcg cgtcaggcgg gagcagcgaa
     3421 aaacgccacg ttgccctctg gaatgcagca actgaacatt gtcccaggtg cgcttcccga
     3481 ctggctggtt agcacagagg atctgaaaaa tgttagccgc gacccattag ctgaggccag
     3541 acgggtgctg gtggtgggac agggcggaga ggcagataac caggaaatcg ttatgctggc
     3601 agaaaagctg ggagccgaag tgggctacag tcgggcgcga gtgatgaacg gtggcgttga
     3661 tgctgaaaaa gtgattggta tttccggaca tctgctggca cctgaggtgt gcattgttgt
     3721 tggcgcttcc ggcgcagcag cgttaatggc gggcgtacgc aatagcaagt ttatggtggc
     3781 gattaatcat gacgccagcg cagcggtatt ttcacaggca gatattggcg tggttgatga
     3841 ctggaaagtg gtgctggagg cactggtgac aaacattcac gcagaatacc aataacgtgc
     3901 tgccggatga tcctgcctac ggttctggac tggggacctg taggcctgat aagacgcgtt
     3961 aagcgtcgca tcaggcgctg aatgtcggat gtggcgtgcg gccaacaaat caaaacaaat
     4021 tcaattggtt acgattatcc cgtcaaagca attttttcgc taacgtccag actccggtat
     4081 tcgtggtgga aagcgccacg acacccgcgt taatcgcatt acgcgcatct tcttcatcgc
     4141 ataccagccc gccagcaatc agcggttggc ggattttttc cgtcacccag cccagcactt
     4201 tgggcatgca gcctggcagg atctcaatac aatccggatt cgattgcgca acttacttat
     4261 caatgttgtg aaacgaaatc gaatcaacaa taaacaggtg atgaatacag aaaaaaactt
     4321 ccgctcttgc cgcttttagc atcgatgctt tggtgctgat aattccatcc gcctcggtca
     4381 ccagtttcaa aaactgaatc accacttctt tatttgacgc gccttccagc aaatcaacat
     4441 gaataaaagc atatttcccg gcgttcttaa tctttttaac gatattactg atggtgcaga
     4501 tattgccgta caacacggaa ataaactggc attcagaatc gatggccagt tgcaggctgg
     4561 cattatcttt aacggcagca atcaccggat tctggcggag caggtgtaag aggggcataa
     4621 attatcctta ttatttatcc aaagcggtag gtgatgccga atccggaagg cggatagcgc
     4681 cactgttgta gtgtggattc gtcgcacagc aaacggcagg tgccacactc caggcagccg
     4741 cgatagtcaa tacgtaagtc accttcctgt gtgagagaaa aaagacctac cggacaggcg
     4801 ttaatcaacc gttgtaccat ctgacgctca acggagtcag ccggaacaat gtgcggcgca
     4861 tcagcaacgc gccagagatt acgggctaca gacatcgcag actcctgata acatcgcccg
     4921 ccagatgcca caggccatga cgacgtaaat gatgccagaa taactggcgc agcggtggaa
     4981 caggtttatc accctgatcc cataaatcgc gggaaatatc ctgcattaac gcaggccacg
     5041 cccgatacct gccagggcgt tgcaaaagcg ccggaacatg ctgataacgt tgtagaacat
     5101 cccatagcag gctgcgctct acgttgtgat gataaagcgc aaacagattt tgcggctcgc
     5161 ggtgctggca ggcgcttatt agcgtttgtg ccgccgcctg tgcgccagtc agcgccatat
     5221 ccatgccgcg cacggaaatt ccggtattga cgcaactgcg caacgcatcg cccaccagca
     5281 gccagccgtt accggcgtat tgcaccggca tactgtgcaa gccaccttct ggcaccagat
     5341 gcgcaccata ctccagtgat tccgtgtttt tgataagcgt gcgcactgcc ggatgcgctt
     5401 taaagcgagt cagcagctcg cttgccggaa cacgactttg cgtaagggaa gagagcgggc
     5461 aaacaatccc taacgagagc gtttgttgat tagtataaag aaatgcgccg ccgggtaagt
     5521 catcacagat tccgccgctg aacagcaacg ctgcgccttc gttattctcc agatgaaaac
     5581 gttcttcaat agcggatgtt tccagcgaca gcacttcttt gatccccaac gccatcgctt
     5641 cgccagcagg acgagtcact aacccgtgac gttcagccag gacgctgttg gcaccttctg
     5701 ccagcaccac ataacgggcg cggagaatat cgtcaccaca aataacgcca cagactctgc
     5761 cgttttcttc atacagtgca tccaccgtcg ctccggggat gcattcgaca ccttcttttt
     5821 cggcttcggc aaccagccac ggatcgaacc gtgcacgtaa tacattccag gattcaccgc
     5881 cgggctgtaa gctggaaaac gtcgttgcgc catccggcgt taacagggaa aggctttcgt
     5941 gagtgatgcg tcgttcaaga ggcgcggtca gatgaaattg cgggaggagt tccgcgagtg
     6001 catgggtata taaccgcctg ccggaaagat ttttgctgcc ggggatttca gcgcgttcga
     6061 gtaacaaaac ggataaatcc gctcgcgcgc agcgtaacgc gcaagcggtg cctgcaatac
     6121 cggcaccaat aataataatg tcgcagtcgt cttccatgtc atgccccaga tatccagaac
     6181 acaacaccct aacatagcgt tactaaaggg aaattgaccg ccgacacact cttatcattc
     6241 gccgcgatag atacaccctg cggtgcaggt ttcttttacc atcaccgcac ttaacagcgg
     6301 cacaacgggt ttaacctgat cccaaatcca ttttgctaaa acttcgctgg ttggattttc
     6361 cagacctgga atatcattga gataatggtg atcgaggcgc tcataagttg gcttaaacgc
     6421 cgctttcagt tcagcgaaat cgataatcca gcccgtatgc ggatcgactt ccccggtaat
     6481 ttccagtcgc accataaagg aatgcccgtg caggcgacca catttatgcc cttccgggac
     6541 atgtggtaag cggtgagcgg cttcgaaggt gaaatcttta aataacgtgg tggacatcat
     6601 aatttctcta caggacgata aaaccgccgt agagtaccgg aaaggctgtt ttttcgcatt
     6661 atctaaaacg actttgttgc gcaaaatcgc tgatttatct taatgattgg ctaaattcat
     6721 ttgtttttca ttaggttggt taatctattt tgttgttaaa gactattgct aaaacaggtt
     6781 agtcgatttg gttattagtt atcgctatcc cgtctttaat ccacaccgtt caccccgtta
     6841 accttacctt ctcttctgtt ttatgggcgc tgacagggcg cagaaacagc tttgcttact
     6901 ggaacataac gacgcatgac gacacaggtc ccaccttccg cgttgcttcc gttgaacccg
     6961 gagcaactgg cacgccttca ggcggccacg accgatttaa cgcctaccca gcttgcctgg
     7021 gtttctggct atttctgggg cgtgctcaac cagcagcctg ttgcgcttgc agtgacgcca
     7081 gcgcccgccg cagaaatgcc gggtataacg attatctctg cttcgcaaac cggcaatgcg
     7141 cgccgggttg ctgaagcatt acgcgatgat ttattagcag caaaactgaa cgttaagctg
     7201 gtgaacgcgg gcgactataa attcaaacaa atcgccagcg aaaaactgct catcgtagtg
     7261 acgtcaacgc aaggggaagg ggaaccgccg gaagaagccg tcgcgctgca taagttcctg
     7321 ttctccaaaa aagcgccaaa gctggaaaac accgcgtttg ccgtgtttag cctcggcgat
     7381 agctcttatg aatttttctg ccagtccggg aaagattttg acagcaagct ggcggaactg
     7441 gggggtgaac gcctgctcga ccgtgtcgat tctgatgttg aataccaggc tgctgccagc
     7501 gagtggcgcg cccgcgtggt tgatgcgctt aaatcgcgtg cgcctgtcgc ggcaccttcg
     7561 caatccgtcg ctactggcgc ggtaaatgaa atccacacca gcccgtacag caaagacgcg
     7621 ccgctggtgg ctagcctctc tgttaaccag aaaattactg ggcgtaactc tgaaaaagac
     7681 gttcgccata tcgaaattga cttaggcgac tcgggcctgc gttaccagcc gggtgacgcg
     7741 ctgggcgtct ggtatcagaa cgatccggca ctggtgaaag aacttgtcga actgctgtgg
     7801 ctgacaggcg atgaacctgt cactgtcgag ggcaaaacgt tgcctctgaa cgaagcgcta
     7861 cagtggcact tcgaactgac cgtcaacacc gccaacattg ttgagaatta cgccacgctt
     7921 acccgcagtg aaacactgct gccgctggtg ggcgataaag cgaagttaca gcattacgcc
     7981 gcgacgacgc tgattgttga catggtgcgt ttctccccgg cacagcttga tgccgaagcg
     8041 ctaattaatc tgctgcgccc gctgaccccg cgtctctatt ccattgcctc ctcgcaggcg
     8101 gaagtcgaga acgaagtaca cgtcaccgtt ggtgtggtgc gttacgacgt ggaaggccgc
     8161 gcccgtgccg gtggtgcctc cagcttcctc gctgaccgcg tggaagaaga gggcgaagtc
     8221 cgcgtattta tcgaacataa cgataacttc cgcctgcccg ctaacctgga aaccccggtg
     8281 attatgattg gcccaggcac cggtattgcg ccgttccgcg cctttatgca gcaacgcgcc
     8341 gctgacgaag cgccaggtaa aaactggctg ttctttggca atccgcattt tacggaagac
     8401 ttcctgtatc aggtggagtg gcagcgctac gtcaaagagg gtgtgctgac gcggatcgat
     8461 ctcgcctggt cgcgcgacca aaaagaaaaa gtttacgtac aagataaact gcgcgaacag
     8521 ggcgcggagc tgtggcgctg gatcaatgac ggtgcccaca tttatgtctg cggcgacgct
     8581 aatcgcatgg cgaaagacgt tgagcaggca cttctggaag tgattgccga atttggtggc
     8641 atggacaccg aagcggcgga tgaattttta agtgagctgc gcgtagagcg ccgttatcag
     8701 cgagatgtct actaatgagc gaaaaacatc cagggccttt agtggtcgaa ggaaaactga
     8761 cagacgccga gcgcatgaag cttgaaagca actacctgcg cggcaccatt gcggaagatt
     8821 taaacgacgg tctgaccggc ggctttaagg gcgataactt cctgctgatc cgcttccacg
     8881 gcatgtatca gcaggatgac cgcgacatcc gcgccgaacg tgctgaacag aagctggagc
     8941 cgcgccacgc gatgctgttg cgctgccgtc tgccgggtgg agttatcacc accaaacagt
     9001 ggcaggcgat cgataaattt gccggtgaaa acaccatcta tggcagcatt cgcctgacca
     9061 accgccagac gtttcagttc cacggcattc tgaaaaagaa cgtcaaaccg gtgcaccaga
     9121 tgctgcactc ggtcggtctt gatgcgctgg cgactgctaa cgacatgaac cgtaacgtac
     9181 tctgcacctc gaacccttac gagtcgcagc tgcacgcaga agcgtacgag tgggcgaaga
     9241 agatttctga acatctgctg ccgcgtaccc gcgcgtatgc ggagatctgg ctcgatcagg
     9301 aaaaagtcgc caccaccgat gaagaaccga tccttggtca gacttacctg ccgcgtaagt
     9361 tcaaaaccac ggtagtgatc ccgccgcaga acgatattga tctgcacgcc aacgacatga
     9421 acttcgtggc gatcgcggaa aacggcaagc tggtgggctt caacctgctg gtgggcggtg
     9481 ggctttctat cgaacacggt aacaagaaaa cctacgcccg cacggcgagt gagtttggct
     9541 atctgccgct agagcatacg ctagcggtgg cggaagccgt cgtgacaact cagcgtgact
     9601 ggggtaaccg aaccgatcgt aaaaatgcca aaaccaaata cacactggag cgcgtggggg
     9661 ttgagacgtt taaagcggaa gtggagcgtc gcgcggggat caaatttgaa ccgatccgcc
     9721 cgtatgaatt tacaggacgc ggcgatcgta ttggctgggt taagggcatt gatgataact
     9781 ggcacctgac gctgtttatc gaaaatggtc gcatccttga ttatccgggg cgtccgctga
     9841 aaactggcct gctggagatc gcgaagatcc acaaaggtga tttccgcatt acggcgaacc
     9901 agaatctgat catcgccggt gtgccggaaa gcgagaaagc gaagatcgag aaaattgcca
     9961 aagagagcgg gttaatgaat gccgtcacgc cgcagcgtga aaactcgatg gcctgcgtgt
    10021 cattcccgac ttgcccgctg gcgatggcgg aagcggagcg tttcctgccg tcttttatcg
    10081 acaacatcga taatttaatg gtgaaacatg gtgtcagcga tgagcatatc gtgatgcgtg
    10141 taacaggctg cccgaacggt tgtggtcgcg cgatgctggc ggaagtgggc ctggtgggta
    10201 aagcaccggg tcgctacaac ctgcatcttg gcggcaaccg cattgggaca cgtatcccac
    10261 ggatgtataa agaaaacatc accgagccgg aaatcctggc gtcgcttgat gaactgatag
    10321 ggcgctgggc gaaagagcgc gaagcgggtg aaggcttcgg cgactttacg gtgcgtgcgg
    10381 gcatcattcg cccggtgctc gatccggcgc gcgatttgtg ggattaacca tcagcccggt
    10441 cttgtaggcc tgataagacg cgtgagcgtc gcatcaggca aggcaaacag tgaggaatct
    10501 atgtccaaac tcgatctaaa cgccctgaac gaactgccga aggtagatcg cattctggcg
    10561 ctggcagaaa ctaacgccga actggaaaaa ctggacgctg aaggccgcgt tgcctgggcg
    10621 ctggataatc tgcccggtga atatgtactt tcttccagct ttggcattca ggcggcggtg
    10681 agcctgcatc tggtgaatca gattcacccg gatattccgg tgatcctcac cgacaccggc
    10741 tatctgttcc cggaaaccta ccgctttatt gacgagttaa cggacaaact caagctcaac
    10801 ctgaaagtgt accgcgctac cgaaagcgcc gcctggcagg aagcacgcta cggcaaactg
    10861 tgggaacagg gcgttgaagg cattgaaaag tacaatgaca tcaacaaagt cgaaccgatg
    10921 aaccgggcgc tgaaagaact gaatgcgcaa acgtggtttg ctggcctgcg gcgtgaacaa
    10981 tccggcagcc gtgccaattt accggtgctg gcaattcagc gtggcgtatt taaagtgctg
    11041 ccgattatcg actgggataa ccgaactatt tatcagtacc tgcaaaaaca tggcctgaaa
    11101 tatcacccat tatgggacga aggatattta tcggtgggcg atacccatac aacccgtaaa
    11161 tgggaacccg gcatggcgga agaagaaacg cgtttctttg gcttaaaaag ggaatgtgga
    11221 ttacacgaag ggtaaatatt gccggataaa ttaaacgcct ctgtcagaaa tgatgggggc
    11281 gtttttattt tgcgagcagc attccggcat ttaaaggtga acatcgcagg gaaaattaac
    11341 aggtatagaa tggcgttggg tgcttgaggc tgtctggcgt taagcattcg cgaggttcca
    11401 gatggacaaa agccccaggc gatatttcta tcaacctgag gctcgcgttc gaacgtagca
    11461 attcggatgt tagtctcttc tttgcaggga ggcaagacat gctgacaaaa tatgcccttg
    11521 tggcaatcat cgtactgtgt tgtacagtac tgggattcac gttgatggta ggtgactcgt
    11581 tatgtgagtt gagtatcaga gaacgtggta tggagtttaa ggcagttctc gcttacgaat
    11641 cgaagaagta gccaccacgc gggggtaaaa ccccgcgtac tgaattgtga tggtttggtc
    11701 tcctgcaccc tttttttaaa tttatatttt ttatgtgaat atatagtctc tatttattat
    11761 tatgaatttc aaatgaaaat cattaattta ttcataatgt aaatgtttta ttggctgata
    11821 tatatcatag atagttttta aaatatgcgt aaatatcctt taagtttact gaaggataaa
    11881 aatattgtga ctttctttga tttctgggga aaaaaccgac gtggcgagaa agagggtatc
    11941 gacgactatc accttctttg ctggcatttg ctggatgtgg ccgcaatggg ctatttaatg
    12001 gttaaaagaa attgcttcgg gctggctgat tattttcgtc aattagggat ttctgacaag
    12061 gaacaggcag ctcgattttt cgcctggttg ctgtgctggc acgatattgg aaaatttgcc
    12121 cgctcttttc aacaacttta cctgccccct gaattcaaga ttcaggaagg tgccagaaaa
    12181 aattacgaaa aaatctctca ttcaacgctg ggctactggt tgtggaatca ttatttaagt
    12241 gaatgtcagg agttacttcc ttcatcttcg ctctctccgc gcaaacttag gcgagtgata
    12301 gaaatgtgga tgcccgtgac gacagggcat catggtcggc cccctgatcg tatggatgaa
    12361 ttggataatt ttctgcctga agacaaagct gcagcacgag attttctcct tgaaatcaag
    12421 ccactgtttc cgcgaataga gatccccaca ttctgggatg atgacgaggg cgttgaactt
    12481 ataaaacaac tttcctggta tatctctgca accgtcgtac ttgcagactg gacagggtcg
    12541 tcaacgcgat tttttccacg cgtcgcacag gcaatggata ttgaagatta ctggcaaaag
    12601 gctttagttc aggctcaaaa cgcattaacc gtctttccgc cacaagcaga aaccgcgcct
    12661 ttcaccggaa ttaatacgct gtttcctttt attgagaata cgacaccgtt acagcaaaag
    12721 gtgctggatc tggatatcaa tcagccagga ccacagttgt ttattctgga agatgtgacc
    12781 ggcgcaggta aaacagaagc ggcgcttatc ctggcgcaca ggttgatggc tgcgggtaac
    12841 gctcaggggc tgttttttgg cctgccaaca atggcaacgg ccaatgccat gtacgatcga
    12901 ctggtcaaaa cctggcttgc tttctattcg ccagaggtgt tccccgctcc agcggagata
    12961 aaccggcgca atacggtgcg gttttcccgg aagtaaaccg gacgcactgg atgcgatgat
    13021 gggtatcact tagaattccc cgcccctgcg gtagaactcc cagctcccat tttcaaaccc
    13081 atcaagacgc cttcgccagc tccttcacca acggtagcat tatccgcata acgtcacggc
    13141 agcgacgttc tattcttcca ggaagtgcct tatcaatatg ttgttgatta tccagtctta
    13201 cgtcatgcca gctatttccc gccgggaagg caggtgtttt tgcgcgttgc tgataaccat
    13261 ccttatttcc aagattccag ttagtcgctt ccaccgaaag tacagcaatg cccgctttgt
    13321 cgaatatttc tgcgtcatta caacccccag tgcctttcgg ataattttta ttcaaacccg
    13381 gattggtcgt tgcggctatt ccgtgactgc gtgcaattgc cagcgccctg tcgcgcgtta
    13441 atttccttac tgcttcaggg gtttttacac cgctgttgaa atacaattta tcgccaacaa
    13501 ttaagttatc gagattaatc accagcagcg tatttttctt ttcggtgtca ctcatccgct
    13561 tgagtaaatt ctcagcgcct aattttcctt cctcttcgcc gctggtcgcc acaaatcgaa
    13621 taccatactc ggtaggcgta tttttcaggc gttctgccag ttccagcatg acacctaaac
    13681 ctgcggcgtt atcatctatt ccttgtaacg tcagtccgcc gagattggca tctgcgtcag
    13741 catcgctcag cggggcgtaa gtatccagat gcgccataat gatgatctgc tgcggcgctt
    13801 tgccttcatg agcggcaatc accgtacttc ctgtcacgtt atgccagttc ttgcgattat
    13861 cgcgggcggt ataaatatac cgactattaa atgtccgaat atcactgcga taacccattt
    13921 gctgaaactg ttggcgaata taatcggcag ataacatttc tgctggagtt ccggtcatgc
    13981 gtcccggaaa gaaagtagca atatgtcgtg cctgggtatt agcaaaatcg ccaggtttag
    14041 gtgacgaggc gtgtacgggg agaataaagc atacgccgag cgccagggca gcggtacggt
    14101 ggcgcaatgc ggaaaacata gtgagtcctt aaataccatg caaatttttt taccgccata
    14161 gtatgaaact gccgctgcgc taaaacaatt tcaaatcttc ctaaacgccc gaaatccggt
    14221 gccttaagca ctttttgata ttagctttgc caaatcgtta ttccgttaag gaactactca
    14281 ttctaattgg taatttcatt cgttctctta cgctccctat agtcgaaaca tctgatggca
    14341 agaaaatagc ggtattgcaa aggaacggtt atggctcaaa aacgacttac tcacctgcgg
    14401 caactggagg cggaaagcat ccacattatt cgcgaggtgg cggcagaatt ctcaaatccg
    14461 gtgatgctct actctatcgg taaagattcc agcgtcatgc tgcatctggc gcgcaaggcg
    14521 ttttatccag gtacgctgcc tttcccgttg ctgcatgtcg ataccggctg gaaattccgc
    14581 gagatgtatg agttccgcga tcgtacggcg aaagcctacg gctgcgagct gctggtgcat
    14641 aaaaacccgg aaggcgtggc gatggggatt aatccattcg tgcacggcag cgcgaaacat
    14701 accgatatta tgaaaactga aggcctgaaa caggcgctga acaaatacgg ttttgatgcc
    14761 gccttcggtg gtgcgcgccg tgacgaagag aaatcccgcg ctaaagagcg aatctactct
    14821 ttccgtgacc gcttccatcg ctgggatccg aaaaatcagc gcccggagct gtggcacaac
    14881 tacaacgggc aaattaacaa aggcgaaagc atccgcgtct tcccgctctc taactggacc
    14941 gagcaggata tctggcaata catctggctg gaaaatatcg acattgtgcc gctgtatctc
    15001 gctgcggaac gtccggttct ggaacgcgac ggtatgttga tgatgattga tgacaaccgt
    15061 atcgacctgc aaccgggcga agtgatcaaa aaacggatgg tgcgtttccg tacgctgggc
    15121 tgctggccgc tgaccggtgc ggtggagtca aatgcacaaa cactgccgga aatcatcgaa
    15181 gagatgctgg tttccaccac cagtgaacgt cagggtcgcg tgattgaccg cgaccaggcg
    15241 ggatccatgg agctgaaaaa acgtcagggg tatttttaag atgaacaccg tacttgcaca
    15301 acaaatcgcc aatgaaggcg gcgtcgaagc ctggatgatt gcgcaacaac ataaaagcct
    15361 gctgcgtttt ctgacctgtg gtagcgtcga tgacggcaaa agtaccctga ttggtcgtct
    15421 gctgcacgat acccgccaaa tctacgaaga tcagctctca tcgctgcata acgacagtaa
    15481 gcgtcacgga acccagggcg aaaagctgga tctggctctg ctggtggacg gcctgcaagc
    15541 tgagcgcgaa cagggcatca ctattgatgt ggcctaccgc tatttctcta ccgagaagcg
    15601 taaatttatt atcgccgaca ccccagggca cgagcagtac actcgcaata tggcgactgg
    15661 cgcatcgaca tgtgaactgg cgatcttact gattgatgcc cgtaaaggcg tgctcgatca
    15721 aacccgtcgt cacagtttta tctccacact gttggggatc aaacatctgg tcgtggcgat
    15781 caacaaaatg gatctggtgg attacagtga agagacgttc acccgtattc gtgaagatta
    15841 tctgaccttt gccgggcagc tgccgggtaa tctggatatc cgctttgtgc cgctctccgc
    15901 actggaaggt gacaacgtgg cttcgcaaag tgaaagtatg ccgtggtaca gcggtctgac
    15961 actgctcgaa gtgctggaaa ccgtggagat ccagcgagtg gtggatgccc agccaatgcg
    16021 cttcccggtg cagtacgtta accgcccaaa tctcgatttt cgtggctacg ccggaatgct
    16081 ggcatccggt cgcgtggaag tcgggcaacg tgtaaaagtg ctgccctctg gtgtggaatc
    16141 aaacgtcgcg cggatcgtga cttttgatgg tgatcgcgaa gaagtctttg ccggagaagc
    16201 gatcaccctg gtgctgacgg atgagatcga tatcagccgt ggtgatctgc tgctggcggc
    16261 agacgaagcg ttaccggcgg tgcagagtgc gtcggtggat gtggtatgga tggcggaaca
    16321 gccgctttcc ccgggccaga gttatgacat caaaattgcc ggtaagaaga cgcgtgctcg
    16381 tgttgatggc attcgttatc aggttgatat taataacctt acccaacgcg aagttgaaaa
    16441 cctgccgctg aacggcatcg gcctggtgga tctcactttt gacgagccac tggtgttaga
    16501 tcgctatcag caaaatccgg ttaccggtgg gctgattttt atcgatcgcc tgagcaatgt
    16561 gaccgtgggt gccggtatgg tgcacgagcc agttagccag gcaacagctg cgtcatctga
    16621 attcagtgca ttcgaactgg aattgaatgc cctggttcgt cgccactttc cgcactgggg
    16681 cgcgcgcgat ttgctggggg ataaataatg gcgctgcatg acgaaaacgt cgtctggcat
    16741 agtcatccgg tcactgtgca acaacgcgaa ctacaccacg gtcatcgtgg tgtagtgctg
    16801 tggtttaccg gcctctccgg gtccggtaaa tcaacggtcg ccggggcgct ggaggaggcg
    16861 ttacataaac tcagcgtcag tacgtatctg ctggatggcg acaatgttcg ccacggatta
    16921 tgcagcgatc tcggttttag cgatgccgat cgtaaagaga atatccgtcg cgtcggtgaa
    16981 gtggcgaatt tgatggttga agccggactg gtggtgctga ccgcatttat ctcgccacac
    17041 cgcgccgaac gccagatggt tcgcgaacgc gtaggagaag ggcgctttat tgaagtgttt
    17101 gtcgatacgc cgctggcgat ttgcgaagcc cgcgatccaa aaggcttata taagaaagcg
    17161 cgtgccggtg aactgcgcaa ctttacggga atagattccg tttacgaagc gcctgaatcg
    17221 gcagaaattc atctcaatgg tgaacaatta gtaacaaatt tggtacagca attattagat
    17281 ctgttgagac agaacgatat tatcagatcc tgagacacca ccgggtttcc atgcccggtt
    17341 cgtcaaagta acaggatcaa atatgcgtaa tagccataac attacactaa caaataacga
    17401 cagccttact gaggatgaag aaactacatg gtcactgcct ggtgccgtgg tcggttttat
    17461 ctcctggtta tttgcgctgg cgatgccgat gttgatttat ggctctaaca cgctgttctt
    17521 ctttctctac acctggcctt tctttctggc actgatgcct gtcgcggtag tggtggggat
    17581 tgcgctgcat tcattgatgg acggaaagct acgctacagt attgttttca ccctggtgac
    17641 agtaggcatt atgtttggcg cactgtttat gtggctactg ggctaatttg tactttcttg
    17701 tctcctctgt tcataattca aaccgtaact aataatgaga ttatgttctg cacgccctgg
    17761 gtatacgtaa caatgggcaa atgtggtaca tttgcccgcg ttgtcgcggt atccccaaca
    17821 gaggatgtag agtcgtcttc ggatgcatgg gatgatgatg cagtttttca gggggcagga
    17881 tgggtaaact aacgctgctg ttgctggcta ttctggtctg gctacagtat tcgctgtggt
    17941 tcggtaagaa cggtatacat gactataccc gcgtcaatga tgatgtggcg gcactgcaag
    18001 ctacaaacgc gaaacttaaa gcgcgaaacg atcaactttt tgccgaaatt gacgatctca
    18061 atggcggcca ggaggcgctc gaagagcgtg cgcgtaatga actcagcatg accaggccgg
    18121 gcgaaacttt ttatcgtctg gtgcctgacg cgtcgaagcg cgcacagtct gcggggcaaa
    18181 acaatcgata aatcagcccg ggaattaaca tggcaaccac tcatttggat gtttgcgccg
    18241 tggttccggc ggccggattt ggccgtcgaa tgcaaacgga atgtcctaag caatatctct
    18301 caatcggtaa tcaaaccatt cttgaacact cggtgcatgc gctgctggcg catccccggg
    18361 tgacacgtgt cgtcattgcc ataagtcctg gcgatagccg ttttgcacaa cttcctctgg
    18421 cgaatcatcc gcaaatcacc gttgtagatg gcggtgatga gcgtgccgat tccgtgctgg
    18481 caggtctgaa agccgctggc gatgtgcagt gggtattggt gcatgacgcc gctcgtcctt
    18541 gtttgcatca ggatgacctt gcgcgattgt tggcgttgag cgaaaccagc cgcacgggag
    18601 ggatcctcgc cgcaccagtg cgcgatacta tgaaacgtgc cgaaccgggc aaaaatgcca
    18661 ttgctcatac cgttgatcgc aacggcttat ggcacgcgct gacgccgcaa tttttccctc
    18721 gtgagctgtt acatgactgt ctgacgcgcg ctctaaatga aggcgctacc attaccgacg
    18781 aagcctcggc gctggaatat tgcggatttc atccgcagtt ggtcgaaggc cgcgcggata
    18841 acattaaagt cacgcgcccg gaagatttgg cactggccga gttttacctc acccgaacca
    18901 tccatcagga gaatacataa tgcgaattgg acacggtttt gacgtacacg cctttggcgg
    18961 tgaaggccca attatcattg gtggcgtacg cattccttac gaaaaaggat tgctggcgca
    19021 ttctgatggc gacgtggcgc ttcatgcgtt gaccgatgca ttgcttggcg cggcggcgct
    19081 gggggatatc ggcaaactgt tcccggatac cgatccggca tttaaaggtg ccgacagccg
    19141 cgagctgcta cgcgaagcct ggcgtcgtat tcaggcgaag ggttataccc ttggcaacgt
    19201 cgatgtcact atcatcgctc aggcaccgaa gatgttgccg cacattccac aaatgcgcgt
    19261 gtttattgcc gaagatctcg gctgccatat ggatgatgtt aacgtgaaag ccactactac
    19321 ggaaaaactt ggatttaccg gacgtgggga agggattgcc tgtgaagcgg tggcgctact
    19381 cattaaggca acaaaatgat tgagtttgat aatctcactt acctccacgg taaaccgcaa
    19441 ggcaccgggc tgctgaaagc caatccggaa gactttgtgg tggtggaaga tttgggcttt
    19501 gagcctgatg gtgaaggtga gcatattctg gttagaatcc tcaaaaacgg ctgcaatacc
    19561 cgttttgtgg cggatgcact ggcgaaattc ctgaaaattc attcccgtga agtcagcttc
    19621 gctgggcaaa aagacaaaca tgctgtaacg gaacagtggt tatgtgctcg cgtgccgggc
    19681 aaggaaatgc ccgatctgag cgcctttcaa ctggaaggct gccaggtgct ggagtatgcg
    19741 cggcacaagc gcaagctgcg tttaggggcg ctgaaaggta acgcctttac cctggttctg
    19801 cgcgaagtga gcaatcgcga tgacgttgaa caacgtctga tcgatatttg cgtaaaaggt
    19861 gtaccgaact acttcggtgc ccaacgtttt gggattggcg gtagcaactt gcagggcgcg
    19921 ctgcgctggg cgcaaaccaa tactccggtg cgcgatcgca ataaacggag tttttggttg
    19981 tcggcagccc gcagtgcgtt gtttaatcag attgttgctg agcgcctcaa aaaagcagac
    20041 gttaatcaag ttgttgacgg cgatgcgcta caattagccg gacgtggtag ctggtttgta
    20101 gcaaccaccg aagaactggc ggaattacag cgtcgcgtca acgataaaga gctgatgata
    20161 accgccgcat tgccgggcag tggcgaatgg ggaactcagc gtgaagcgct ggcattcgaa
    20221 caagcagctg tcgccgcaga aactgaatta caagctttac tggtgcgcga aaaagttgaa
    20281 gccgcgcgca gagcgatgct gctgtatccg caacaattaa gctggaactg gtgggatgac
    20341 gtcaccgtag agatccgttt ctggcttccg gcgggtagtt ttgcaaccag cgttgtcagg
    20401 gaacttatca acacaacagg tgattatgcg catattgctg agtaattatg acggggtaca
    20461 tgcacccggt atacaaacgc tggcgaaagc cttgcgtgag tttgctgacg ttcaggtggt
    20521 cgcccccgat cgtaaccgca gcggcgcttc aaattctctg acactggaat cctccctgcg
    20581 cacgtttacc tttgaaaatg gtgatattgc tgtgcaaatg ggaaccccga ccgattgtgt
    20641 ctatcttggc gtgaatgctc tgatgcgtcc gcgcccggac attgttgtgt ccggaattaa
    20701 cgccgggccg aatctggggg atgatgttat ttattccggt acggtagccg ccgcgatgga
    20761 aggccgtcat ttaggttttc cggcgctggc cgtctcgctt gacgggcata aacattacga
    20821 cactgccgcg gcggtaacct gttcaatttt gcgcgcactg tgtaaagagc cgctgcgcac
    20881 cggacgtatt cttaatatta acgttcagga tttacccttg gatcaaatca aaggtattcg
    20941 cgtgacgcgc tgcggtacac gacatccggc agatcaggtg atcccgcagc aagatccgcg
    21001 cggcaatacg ctgtactgga ttggcccgcc gggcggtaaa tgtgatgccg gtccggggac
    21061 cgattttgct gcggtagatg agggctatgt ctccatcacg ccgctgcatg tggatttagc
    21121 tgcgcatagc gcgcaagatg tggtttcaga ctggttaaac agcgtgggag ttggcacgca
    21181 atggtaagca gacgcgtaca agcacttctg gatcaattac gtgcgcaagg tattcaggat
    21241 gagcaggtgc tgaatgcact tgccgccgtg ccgcgtgaaa aattcgttga tgaagcgttt
    21301 gaacaaaaag cctgggacaa tatcgccttg ccgataggtc aggggcagac aatttcgcag
    21361 ccgtatatgg tggcgcgaat gaccgaatta ctcgagctga cgccgcagtc gcgggtgctg
    21421 gaaattggca ctggttcggg atatcaaacg gcaatcctgg cgcatcttgt ccagcatgtt
    21481 tgctcggttg aacggattaa aggcttgcag tggcaggcac gtcgccgcct gaaaaatctt
    21541 gatttacata atgtttcaac ccgtcatggt gatggatggc aaggttggca ggcacgtgcg
    21601 ccgtttgacg ctatcattgt tacggcggca ccgccggaaa ttccaactgc gctaatgacg
    21661 cagctggacg aaggcgggat tctcgtctta cccgtagggg aggagcacca gtatttgaaa
    21721 cgggtgcgtc gtcggggagg cgaatttatt atcgataccg tggaggccgt gcgctttgtc
    21781 cctttagtga agggtgagct ggcttaaaac gtgaggaaat acctggattt ttcctggtta
    21841 ttttgccgta ggtcagcgta tcgtgaacat cttttccagt gttcagtagg gtgccttgca
    21901 cggtaattat gtcactggtt attaaccaat ttttcctggg ggataaatga gcgcgggaag
    21961 cccaaaattc accgttcgcc gcattgcggc tttgtcactg gtttcgctat ggctggcagg
    22021 ctgttctgac acttcaaatc caccggcacc ggtcagctcc gttaatggca atgcgcctgc
    22081 aaatactaat tctggtatgt tgattacgcc gccgccgaaa atggggacga cgtctacagc
    22141 gcagcaaccg caaattcagc cggtacagca gccacaaatt caggctactc aacaaccgca
    22201 aatccagcca gtgcagccag tagctcagca gccggtacag atggaaaacg gacgcatcgt
    22261 ctataaccgt cagtatggga acattccgaa aggcagttat agcggcagta cctataccgt
    22321 gaaaaaaggc gacacacttt tctatatcgc ctggattact ggcaacgatt tccgtgacct
    22381 tgctcagcgc aacaatattc aggcaccata cgcgctgaac gttggtcaga ccttgcaggt
    22441 gggtaatgct tccggtacgc caatcactgg cggaaatgcc attacccagg ccgacgcagc
    22501 agagcaagga gttgtgatca agcctgcaca aaattccacc gttgctgttg cgtcgcaacc
    22561 gacaattacg tattctgagt cttcgggtga acagagtgct aacaaaatgt tgccgaacaa
    22621 caagccaact gcgaccacgg tcacagcgcc tgtaacggta ccaacagcaa gcacaaccga
    22681 gccgactgtc agcagtacat caaccagtac gcctatctcc acctggcgct ggccgactga
    22741 gggcaaagtg atcgaaacct ttggcgcttc tgaggggggc aacaagggga ttgatatcgc
    22801 aggcagcaaa ggacaggcaa ttatcgcgac cgcagatggc cgcgttgttt atgctggtaa
    22861 cgcgctgcgc ggctacggta atctgattat catcaaacat aatgatgatt acctgagtgc
    22921 ctacgcccat aacgacacaa tgctggtccg ggaacaacaa gaagtgaagg cggggcaaaa
    22981 aatagcaacc atgggtagca ccggaaccag ttcaacacgc ttgcattttg aaattcgtta
    23041 caaggggaaa tccgtaaacc cgctgcgtta tttgccgcag cgataaatcg gcggaaccag
    23101 gcttttgctt gaatgttccg tcaagggatc acgggtagga gccaccttat gagtcagaat
    23161 acgctgaaag ttcatgattt aaatgaagat gcggaatttg atgagaacgg agttgaggtt
    23221 tttgacgaaa aggccttagt agaagaggaa cccagtgata acgatttggc cgaagaggaa
    23281 ctgttatcgc agggagccac acagcgtgtg ttggacgcga ctcagcttta ccttggtgag
    23341 attggttatt caccactgtt aacggccgaa gaagaagttt attttgcgcg tcgcgcactg
    23401 cgtggagatg tcgcctctcg ccgccggatg atcgagagta acttgcgtct ggtggtaaaa
    23461 attgcccgcc gttatggcaa tcgtggtctg gcgttgctgg accttatcga agagggcaac
    23521 ttggggctga tccgcgcggt agagaagttt gacccggaac gtggtttccg cttctcaaca
    23581 tacgcaacct ggtggattcg ccagacgatt gaacgggcga ttatgaacca aacccgtact
    23641 attcgtttgc cgattcacat cgtaaaggag ctgaacgttt acctgcgaac cgcacgtgag
    23701 ttgtcccata agctggacca tgaaccaagt gcggaagaga tcgcagagca actggataag
    23761 ccagttgatg acgtcagccg tatgcttcgt cttaacgagc gcattacctc ggtagacacc
    23821 ccgctgggtg gtgattccga aaaagcgttg ctggacatcc tggccgatga aaaagagaac
    23881 ggtccggaag ataccacgca agatgacgat atgaagcaga gcatcgtcaa atggctgttc
    23941 gagctgaacg ccaaacagcg tgaagtactg gcacgtcgat tcggtttgct ggggtatgaa
    24001 gcggcaacac tggaagatgt aggtcgtgaa attggcctca cccgtgaacg tgttcgccag
    24061 attcaggttg aaggcctgcg ccgtttgcgc gaaatcctgc aaacgcaggg gctgaatatc
    24121 gaagcgctgt tccgcaagta agtaagcatc tgtcagaaag gccagtctca agcgaggctg
    24181 gcctttttct tttgtttggg aagcttgtgt ttttgtcgcc tggataagac acgtcagcgt
    24241 cctacccggc aatcagcatt ctatgtgctc atcatcttgc gcaccaactg cataaacagc
    24301 tcctgctctt ctccactcaa acgccccaaa aattcctcat ccacgctgtc accaataggt
    24361 atcgccgcag caagtagctt ttctccttcg gcagtcagcc agacaaagcg tcgccgcttg
    24421 tcagcgacgt catgttcacg tctgactaag ccacgatttt ccattcttgc caacatttct
    24481 gctagcgttg ctttggtgct gactgctgct tctatcagtg ccacctgttc aataccaggt
    24541 ttatcagcaa tggcgcgcat caccgcgtac tgtggtttgg tcaggtcagg taactcgtgc
    24601 tgccagcgag ccgtgtgctg ctgaaaaagc tgtcgtaact gatggaacgc tttatttcgt
    24661 aacgccatgt aaactccggg ctaattctct gtcgctatca tagcggtttt ttagtgacga
    24721 agaagatgtt gttacttttc aatccttgcc gctgtggaaa tgaaggagta tgttaataat
    24781 aacgttcgta tacgaataat taagagaata agcaatgaaa ctgatcgtcg ggatgacagg
    24841 ggctaccggt gcgcctcttg gtgtggcatt actgcaagcg ctgcgtgaga tgccgaatgt
    24901 cgagactcat ctggtgatgt cgaagtgggc gaaaaccacc attgaactgg aaacgcctta
    24961 cagcgctcgc gatgttgctg ccctcgcaga cttcagccat aacccggcgg atcaggcggc
    25021 gatcatctca tccggttctt ttcgtaccga cggcatgatc gttattccgt gcagtatgaa
    25081 aacgctcgcc ggtatccgcg ctggttacgc cgatggcctg gtagggcgcg cggcggacgt
    25141 cgtgctcaaa gaaggccgca aactggtgct ggtgccgcgt gaaatgccgc ttagcaccat
    25201 ccatctcgaa aatatgctcg cactttcacg catgggcgtg gcgatggtgc cgccgatgcc
    25261 tgccttttat aaccatcccg aaacggtaga tgacattgtc caccatgtgg tagcccgcgt
    25321 gctggatcaa tttggcctcg aacatcccca cgccaggcgc tggcaaggat tgccgcaggc
    25381 ccggaatttt tctcaggaga atgaataatg gcatttgatg atttacgcag ctttttacag
    25441 gcgcttgatg accacggcca gttactgaaa atcagcgaag aagtgaacgc tgagccggat
    25501 ctggcagcag cagctaacgc caccgggcgt atcggcgacg gcgcgcccgc gctgtggttt
    25561 gataatattc gcggctttac cgatgcccgc gtggcgatga ataccatcgg ttcctggcag
    25621 aaccacgcga tttcccttgg cctgccacca aacaccccgg tcaaaaagca gattgatgag
    25681 tttatccgcc gctgggataa cttcccgatt gccccggagc gccgcgccaa tccagcctgg
    25741 gcgcagaaca ccgtcgatgg cgaagagatc aacctgttcg atattctgcc gctgtttcgc
    25801 ctgaatgatg gcgatggcgg tttctatctc gacaaagcgt gcgtggtttc ccgcgatccg
    25861 ctcgacccgg ataacttcgg caagcagaac gtcggcatct accgcatgga agtgaagggc
    25921 aagcgtaagc tcggcctgca accggtgccg atgcacgata tcgccctgca tctgcataaa
    25981 gcagaagagc gcggtgaaga tctgccgatt gcgatcacgc tcggtaacga tccgatcatc
    26041 acgctgatgg gggccacgcc gctgaaatac gatcagtctg agtatgaaat ggcaggcgcg
    26101 ctgcgtgaaa gcccgtaccc gatcgccacc gctccgctga ccggttttga tgtgccgtgg
    26161 ggttcagaag tgatcctcga aggggttatc gaaagccgta aacgcgaaat cgaagggccg
    26221 ttcggtgagt ttaccgggca ctactccggt gggcgcaaca tgaccgtggt gcgcatcgat
    26281 aaagtctctt atcgcagcaa accgattttc gaatcactct atctcggtat gccgtggacc
    26341 gaaatcgact acctatgact aggtaattta ttcaagataa ccgggaagag attaaaaaag
    26401 acgggaatag attggaatca acaagttagg aactcaaggt tttcaagata aagcgagtcc
    26461 aaaaatgtta aacggttcga tatcaataaa acattcaaga taactcacag gttcttacaa
    26521 tagcctttaa agaccgttca aaaacgattc aggtgtttat tatagcgctg atgggtgcaa
    26581 gcgccaaaac caatgtaatt ttagcacagc taggcacttg cactgggtta tgcgctggct
    26641 gctttctgag gtagatttaa gtgatcgccc ttagcttcgg acaacgcgta aatctctcta
    26701 accttttcgt ctggcaaatt accaaggctt aacgctatgg ctctacgaac tccaactaga
    26761 gcgtgcaact cttggatgtg ctcatccaga agaatgagta attgctccgc tccttttctc
    26821 ccaagaacat cagctacttg ttgtttttct tgtggtttaa gaaatgacaa gaacattaga
    26881 atttgagcat caacattatc cgcattctct tttttgggtt ctttggaatc ttttagttca
    26941 acatcaccct ctcccgtcat gagccattcc aaggtaacat tttcgctttc tgctatcttt
    27001 tttaactgat acgggcgtgg tatcggtgcg taatcccgcc tgttgtaata attttttaaa
    27061 gtgccttcat tgatccccca tgcccgagca gccgcatttc ttgaagggta tcgttctact
    27121 aacctaaaga ttctttcgat aatcggttct tttccatcgg caggaaaaga accttggggt
    27181 gcttttctca ttcttggttc ctttctatct aattcattga taaataataa aattaaaaaa
    27241 taaaccataa aaacaccaaa gaaccctttg cagggaatct ttggtatctt aatctttatc
    27301 catacggata tccgaccgga tatccgcgcg ggtaattttg taggttaaag gaaagatgag
    27361 acaaagagaa gatagtgacc gcgattggcc ttcgctgaag attcagctgg cgctcaaaga
    27421 taacgggtgg tcttccttcc gggagctttc actccatgct ggttatgctc aggattcact
    27481 gaaagcggta gttcgcacac caaacaaaaa atacgaacgc gtaatttctg acgctatagg
    27541 tgtaccgcca gaaatcatct ggcccagccg gtatcgtaac gatggattta tcaggaaggc
    27601 gtcgtgatat gcagtcgtac gttaccgtaa atgacctgct cggcgtacca ggaatgccag
    27661 ccaccacaaa aggcatccga caggcgttac agcgtttttc tcgtgatctt ggtgacgtta
    27721 gtcgccgtcg ggaaggcaca aaagccattg agtaccacat tgattgtctg cctgaaataa
    27781 cacgaaaggc actgcgtgaa cgctatgtgg aacagctggt ggctacagag aacaatgttt
    27841 ctgaagtgaa agctgttacc agaaaaacac gcaatcctga cgctgtccag gcaatcgaag
    27901 catatcgcgg ttctccacaa ctgatggaag aacgcctgaa tgcgctgacc gaaaaccagc
    27961 gctgggtatc tgaagcaaga gctgcgctgg tggtggaagt gctgaagctg gaaagcgccg
    28021 gtaaccccgg gcgactgaaa gccattaact ttcttgttga aaaagcccgt aaaggtgagc
    28081 tgccggagcg cctgcaacag gccgcagtta acgccaatgc aaaacgtggc gctaatcgta
    28141 ctatcagccg cgatccactt taccagtggg ttctgaaata caaccagtcg cagaacgccg
    28201 ccgaacggct gctgttactg gcaccgggaa aacgcgacga aataaaaccg gaagaaatca
    28261 gctggctgcc tgaatttctg gcgcagtatc gccaggtgaa cggcagaccc atgtcagaag
    28321 cctacgagga ttttgtcgca gaatggcagc gtcgccatgc ggacgaaccg tacatgctgg
    28381 aggtgatgcc ttcctatgat gttgtccggt atgccatgaa aaaactgccg gaagtggtga
    28441 aacaaaaagg gcgcgttacc ggcagtgaat accgtcagct tgaaggattc acccgccgcg
    28501 actggacagc tatgccggtg aattatgtct ggattggtga cggtcacggt atgaagctga
    28561 aatgtgcgca tccgatccac gggcgtccat tctcaccgga agtcacgttt gtgatcgatg
    28621 gtggaacacg gtttgttgtt ggctggagtc ttgaccttgc cgaaaacgtg ttcgccgttg
    28681 ccggtgcgat acagcacggc attcgcaacc acggaaaacc gttcctgtat tactcggata
    28741 acggttccgg tgaaacggct gacatgctgg acaaagaggt cgtgggtatt ctgccacgcc
    28801 tggggattaa acaccctacc ggtattgccg gtaatccaca gggacgcggc attattgaac
    28861 gcctgaaccg gacactgccg atgcgtatag ccaggaggta tcgcacctat tttggcaagg
    28921 gtgctgaccg ggaatcgttg cgtgtactta accgtgatct gcgttcagcg tttaacgccc
    28981 tgcaacagga caagcctctg aacgaccgcc agaaagcggc aatgcgcgag ctgccatcgt
    29041 gggcggagct tattgaggct attcgtgaag gcgtggaatg gtacaacaac cgcccgcatt
    29101 ctgagctgcc gatgaaaccg aacggacggc attacagccc gacagagttc agaaaaaaac
    29161 gccaggcaga agaagacacc gaaattgagt ggctgagtga tcttgagctg cgcgacatgt
    29221 tccgcccaat ggtggaacga ccggtaagac gctgcgaaat tcagtggctg aacaatattt
    29281 actacgcgcc ggagttgcgt gacgaacacg gtcgcaaagt gcttatcagt tatgacattc
    29341 atgacgccga gcgaattacg gtacgtcgca aggacggcag ctttatctgt gaggcgatat
    29401 ggaacggcaa taaacgcgca gcctttgctg tcagtgctga atatcacaaa caacagcagc
    29461 gtattaaagg aatgcgcaaa cgcgctgagg aaaaaattcg tgatgctgaa gatgaaggta
    29521 ttcagattct ggaacacaaa caggctgaac cctggctgag taatgtttat cgcccggttg
    29581 gtaatgtggt ggcagttcag caaccggaat atgaagaaga gcatgacgaa gaatttgaac
    29641 gcgatttccg gctggggatg caaaaacttt ttgccatgca ggaagaagat gatccgctgg
    29701 cctgaataaa aaaacagtcc gcgggaacgg actgttaaat aaaggaaaca aagctgattt
    29761 gaaataaccg aggaaatact atgactgata ttgatgatg
//
LOCUS       DAJTSI010000038        29466 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000038 DAJTSI010000000
VERSION     DAJTSI010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29466
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.038"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..213)
                     /locus_tag="OO327_001497"
     CDS             complement(<1..213)
                     /locus_tag="OO327_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCR6018085.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
     gene            523..2295
                     /gene="aspS"
                     /locus_tag="OO327_001498"
     CDS             523..2295
                     /gene="aspS"
                     /locus_tag="OO327_001498"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR6018086.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2413..2865
                     /gene="nudB"
                     /locus_tag="OO327_001499"
     CDS             2413..2865
                     /gene="nudB"
                     /locus_tag="OO327_001499"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR6018087.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2894..3634
                     /locus_tag="OO327_001500"
     CDS             2894..3634
                     /locus_tag="OO327_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6018088.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3669..4190
                     /gene="ruvC"
                     /locus_tag="OO327_001501"
     CDS             3669..4190
                     /gene="ruvC"
                     /locus_tag="OO327_001501"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR6018089.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4192..4794)
                     /locus_tag="OO327_001502"
     CDS             complement(4192..4794)
                     /locus_tag="OO327_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCR6018090.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4864..4929
                     /gene="yobI"
                     /locus_tag="OO327_001503"
     CDS             4864..4929
                     /gene="yobI"
                     /locus_tag="OO327_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR6018091.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5068..5679
                     /gene="ruvA"
                     /locus_tag="OO327_001504"
     CDS             5068..5679
                     /gene="ruvA"
                     /locus_tag="OO327_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR6018092.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            5688..6698
                     /gene="ruvB"
                     /locus_tag="OO327_001505"
     CDS             5688..6698
                     /gene="ruvB"
                     /locus_tag="OO327_001505"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR6018093.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6845..7630)
                     /gene="znuB"
                     /locus_tag="OO327_001506"
     CDS             complement(6845..7630)
                     /gene="znuB"
                     /locus_tag="OO327_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR6018094.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7627..8382)
                     /gene="znuC"
                     /locus_tag="OO327_001507"
     CDS             complement(7627..8382)
                     /gene="znuC"
                     /locus_tag="OO327_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR6018095.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8461..9393
                     /gene="znuA"
                     /locus_tag="OO327_001508"
     CDS             8461..9393
                     /gene="znuA"
                     /locus_tag="OO327_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR6018096.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTSLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9409..10731
                     /gene="mepM"
                     /locus_tag="OO327_001509"
     CDS             9409..10731
                     /gene="mepM"
                     /locus_tag="OO327_001509"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR6018097.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10851..11822
                     /gene="lpxM"
                     /locus_tag="OO327_001510"
                     /gene_synonym="msbB"
     CDS             10851..11822
                     /gene="lpxM"
                     /locus_tag="OO327_001510"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6018098.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(11953..13395)
                     /gene="pyk"
                     /locus_tag="OO327_001511"
     CDS             complement(11953..13395)
                     /gene="pyk"
                     /locus_tag="OO327_001511"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR6018099.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMVSMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13523..14392)
                     /gene="hexR"
                     /locus_tag="OO327_001512"
                     /gene_synonym="yebK"
     CDS             complement(13523..14392)
                     /gene="hexR"
                     /locus_tag="OO327_001512"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR6018100.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14729..16204
                     /gene="zwf"
                     /locus_tag="OO327_001513"
     CDS             14729..16204
                     /gene="zwf"
                     /locus_tag="OO327_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR6018101.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16439..18250
                     /gene="edd"
                     /locus_tag="OO327_001514"
     CDS             16439..18250
                     /gene="edd"
                     /locus_tag="OO327_001514"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR6018102.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            18287..18928
                     /gene="kdgA"
                     /locus_tag="OO327_001515"
                     /gene_synonym="eda"
     CDS             18287..18928
                     /gene="kdgA"
                     /locus_tag="OO327_001515"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR6018103.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(18984..20162)
                     /gene="purT"
                     /locus_tag="OO327_001516"
     CDS             complement(18984..20162)
                     /gene="purT"
                     /locus_tag="OO327_001516"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR6018104.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRHLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            20296..20586
                     /gene="yebG"
                     /locus_tag="OO327_001517"
                     /pseudo
     CDS             20296..20586
                     /gene="yebG"
                     /locus_tag="OO327_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            20653..21009
                     /locus_tag="OO327_001518"
     CDS             20653..21009
                     /locus_tag="OO327_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR6018105.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            21336..21995
                     /locus_tag="OO327_001519"
     CDS             21336..21995
                     /locus_tag="OO327_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR6018106.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            22205..24265
                     /gene="ptrB"
                     /locus_tag="OO327_001520"
     CDS             22205..24265
                     /gene="ptrB"
                     /locus_tag="OO327_001520"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR6018107.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTALAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(24262..24924)
                     /gene="exoX"
                     /locus_tag="OO327_001521"
     CDS             complement(24262..24924)
                     /gene="exoX"
                     /locus_tag="OO327_001521"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR6018108.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(24948..25604)
                     /locus_tag="OO327_001522"
                     /pseudo
     CDS             complement(24948..25604)
                     /locus_tag="OO327_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            complement(25706..25936)
                     /gene="holE"
                     /locus_tag="OO327_001523"
     CDS             complement(25706..25936)
                     /gene="holE"
                     /locus_tag="OO327_001523"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR6018109.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            26075..26449
                     /gene="yobA"
                     /locus_tag="OO327_001524"
     CDS             26075..26449
                     /gene="yobA"
                     /locus_tag="OO327_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR6018110.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            26453..27325
                     /gene="copD"
                     /locus_tag="OO327_001525"
                     /pseudo
     CDS             26453..27325
                     /gene="copD"
                     /locus_tag="OO327_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            27338..27679
                     /locus_tag="OO327_001526"
     CDS             27338..27679
                     /locus_tag="OO327_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR6018111.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(28072..29149)
                     /locus_tag="OO327_001527"
                     /pseudo
     CDS             complement(28072..29149)
                     /locus_tag="OO327_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
     gene            complement(29115..29396)
                     /locus_tag="OO327_001528"
     CDS             complement(29115..29396)
                     /locus_tag="OO327_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR6018112.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
BASE COUNT         7437 a         7233 c         7568 g         7228 t
ORIGIN      
        1 atcaaccggc tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa
       61 cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc
      121 atcggcagta tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac
      181 caacgcagtg gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg
      241 cctcacacct taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag
      301 ttcgggcaac acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt
      361 ggtaacgagg cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa
      421 tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg
      481 cagcaggggg catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt
      541 ggacagctcc gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt
      601 cgtcgtgatc ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag
      661 gtatttttcg atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag
      721 ttctgcattc aggttacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat
      781 atggcgaccg gcgaaattga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat
      841 gttctgccgc ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac
      901 ctcgacctgc gtcgcccgga aatggcgcaa cgcctgaaaa cccgcgctaa aatcaccagc
      961 ctggtgcgcc gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc
     1021 aaagccacgc cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa
     1081 ttctacgcac tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt
     1141 gaccgttact atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag
     1201 cctgaattta ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa
     1261 gtgatggaag cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat
     1321 ttcccggtaa tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg
     1381 cgtaacccga tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta
     1441 tttgcggggc cagcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc
     1501 gcatcgctga cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg
     1561 aaaggtctgg cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc
     1621 ccggtagcga agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg
     1681 caagatggcg atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgggatg
     1741 ggcgcactgc tcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca
     1801 ccgctgtggg ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca
     1861 atgcaccatc cgttcacctc accgaaagat atgactgctg cagaactgaa agctgcaccg
     1921 gaaaatgcgg tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt
     1981 tcagtacgta tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac
     2041 gaagaggaac agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg
     2101 ccgcacgcag gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac
     2161 aatatccgtg acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa
     2221 gcaccgagct ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag
     2281 gctgagaata actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca
     2341 actgagtgaa ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa
     2401 cgaagaggca gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc
     2461 tatgcacaag atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg
     2521 cagtcggtaa ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa
     2581 gtaaaggaag aggttaccat tgatgttgtc gctgaacaac tgaccttaat tgactgtcag
     2641 cgcacggtag agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg
     2701 cgtaatacgg aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact
     2761 gaacatctgg cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg
     2821 agcaaccggc aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc
     2881 tggagatatt tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc
     2941 gcaggatgct aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc
     3001 taagctgggc ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc
     3061 actgtctaac aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg
     3121 tgatgatgat gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc
     3181 aatcatgatt gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcacgc
     3241 attcagcaaa tgtggcggta acctcggcac tgacggttcc gtagcttatc tgttcagcaa
     3301 aaaaggcgtg atctccttcg agaaaggcga cgaagacacc atcatggaag cagctctgga
     3361 agcaggtgct gaagacgttg tgacatatga tgacggcgcg attgatgtct acactgcatg
     3421 ggaagaaatg ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc
     3481 ggaagtttcc atgatcccgt ccaccaaagc tgatatggat gcagaaaccg cgccgaaact
     3541 gatgcgtctg atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg
     3601 tgaaatctct gatgaggttg cagcgactct ctgatgatgc ctgctaaaca gcaaaacgga
     3661 gacgcgtgat ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctacggcg
     3721 tcatccgcca ggtaggtagg caactgtcct acctgggtag cggatgcata cgcaccaaag
     3781 tggatgattt accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc
     3841 agttccagcc tgattatttc gccattgaac aagtctttat ggcgaagaac gctgactcag
     3901 ccctgaaact gggccaggcg cgcggcgtgg cgattgttgc ggcggtgaat caggaattgc
     3961 cggtatttga atacgcggca cgtcaggtaa agcaaacggt ggtaggtatg ggtagtgccg
     4021 aaaaaagcca ggtgcagcat atggtccgca ccttgctgaa actgcccgct aatccacagg
     4081 cggatgccgc cgatgcgctg gcgattgcca tcacccactg ccacgttagt cagaatgcga
     4141 tgcagatgag cgaatcgcgg ctgaacctga cgagagggcg actgcgttaa gttataccgc
     4201 ctcggtcagt tccggctgag gcgtttcaac tccctccgcg tgaatcaaca ccaaacccgg
     4261 atgatgccac aaactggctg gcgtgacggt tttacgctcc cacggaatag aacctaagaa
     4321 ccagaatttc cagaaagtga gttttgcatt cggattgcta tttaacagtt gtccaaacgt
     4381 ctctatttca cccaccggaa tacatagcgc ctctttataa atatcaaaga caaattttga
     4441 acagaactgg cgcgaagatt cgtatttaaa acccgtgtgg taaagtttgc gtagccggga
     4501 gggaacctgt tcaacaattc gttgtttttg ttgttctgtc agcccagcgt ctaatcgctt
     4561 tatagcatag cgttgattag acgaacgttt aataaaacga gatagcgtgg tgatggttga
     4621 aaggggaacc cggctttctg caaccaggaa gtcttcaccg ttatgaccga taatgatccc
     4681 gacgtgatta ctccagcaat ttgatgcagc tgatatttga ccaaataagg cagcacctat
     4741 acatgtaaag acgatatctc caatttcata ttcagcaggg taattaatat tcacgttgtc
     4801 attccattga aatagataca aatatacgta atagcataaa aaaattattt ttcaggagtc
     4861 gcaatgtata tttttattac gcatttcttc actgaatatg taatattcaa atatttgctt
     4921 ccaatataac ctgttgaata aattatactg tgccattttt cagatcatcg ggacacctcg
     4981 caagttttct tcatccttcg ctggatatct atccagcatt tttttatcat acagcattat
     5041 ctttgattca ttacgcagga gcgtcatgtg ataggcagac tcagaggcat catcattgaa
     5101 aaacaacccc cgctggtgtt aattgaagtg ggcggcgtag gctatgaagt gcatatgccg
     5161 atgacctgtt tttatgaact ccctgaagcg ggtcaggaag cgatcgtttt cacccacttt
     5221 gtggtgcgtg aagacgcgca actgctgtac ggttttaaca ataaacaaga acgcacgttg
     5281 ttcaaagagt tgatcaaaac caacggcgtc ggcccgaagt tggccctggc gatcctctcc
     5341 ggaatgtcag cgcagcagtt cgttaatgcc gttgagcgtg aagaagtggg ggcactggtg
     5401 aaactgccgg gtattggcaa aaaaaccgcc gaacgcttga ttgttgaaat gaaagaccga
     5461 tttaaaggtt tgcatggcga tctctttacg ccagccgccg acctggtact cacgtctcct
     5521 gctagcccgg cgaccgacga tgctgaacaa gaagcagttg ccgcgctggt ggcgctaggc
     5581 tataaaccac aagaagcaag ccgcatggtg agcaaaatcg ctcgccctga caccagcagt
     5641 gaaacattaa ttcgcgaagc cctacgcgcc gcgttatgag gtaaaggatg attgaagcag
     5701 accgtctgat ttctgccggt accactttgc cggaagatgt agcagatcgc gccattcgcc
     5761 ctaaattact ggaagagtat gttggtcagc cgcaggttcg ttcacagatg gagattttca
     5821 tcaaagcagc gaaactgcgc ggcgatgccc tcgatcattt gttgattttt ggtcctccgg
     5881 ggttgggtaa aactacgctt gccaacattg tcgccaatga aatgggcgtt aatttacgca
     5941 cgacttctgg tccggtgctg gaaaaggcgg gcgatttggc tgcgatgctc actaaccttg
     6001 aaccgcatga cgtgctgttt attgatgaga tccaccgttt atcgccagtt gttgaagaag
     6061 tgctgtaccc ggcaatggaa gactaccaac tggatatcat gattggtgaa ggtccggcgg
     6121 cacgctccat taaaattgat ttgccgccgt ttaccctgat tggcgcaacc acgcgcgcag
     6181 gttcgctgac atcaccgttg cgcgaccgtt ttggtattgt gcaacgtctg gagttttatc
     6241 aggtgccgga tctgcaatat atcgtcagtc gcagtgcacg ctttatgggg cttgagatga
     6301 gtgatgacgg tgcgcaggaa gtagctcgtc gcgctcgcgg tacgccacgt attgccaacc
     6361 gtctgctgcg tcgagtgcgt gatttcgccg aagtgaagca cgatggcacc atctcggcag
     6421 atatcgctgc tcaggcgctg gatatgttga atgtcgatgc tgaaggtttc gattatatgg
     6481 accgcaaatt gttgctggcg gtaatcgata agttctttgg tgggccggta ggtctggata
     6541 acctggcggc agccattggc gaagaacgtg aaaccattga agatgtactg gaaccttatt
     6601 tgattcagca aggctttttg cagcgtacac cgcgcgggcg tatggcgacg acgcgggcgt
     6661 ggaatcactt tggcataacg ccgccagaaa tgccgtaagt cggattggtc gctcgcgccg
     6721 catccgacac tggcagtgtg tctgatgcga cactgttcgc gtcttatcag gccagccaga
     6781 gatagcgaac cgtaggtcgg ataaggcgct cgcgccgcat ccgacaaatg tgttcagcga
     6841 tagattagct ggcctgcttt ttcatcatac tgataataaa taacagtgcc gcacatagca
     6901 ccaccgacgg acccgccggc gtatcgtaaa acgcggaaaa ggttaaaccg ccagttactg
     6961 ccaccatccc caccaaaaca gcgacaccag ccatctgttc cggcgtacgg gcaaagcgac
     7021 gcgcagtagc tgcaggaata atcagcaacg aagtaataat caacgcaccg acaaatttca
     7081 tcgctacacc aatcgtcaat gccgtcacca gcatcaacaa caatttcact tgctgtaatt
     7141 tcacaccatc aacaaacgcc agatccgggc taatcgtcat cgacagcaaa ttgcgccatt
     7201 gccagaacaa aatagccacc acgatgacca cgccaatcgc aatagagatg agatcttctg
     7261 gcgtcactgc cagtaaatca ccgaacaggt aagccatcaa atcaacacga atgttagaca
     7321 tcagactaac gaccactagg cccagcgaca gggcactgtg cgccataatc cctaataacg
     7381 tgtcgatcgc cagctgtgga cgcttctcca gccataccag accgcccgcc agcagcagcg
     7441 taacggcaat caccgcatag aatggattca cgtccagcaa caaaccaaac gcgacaccaa
     7501 gtaatgaggc atgagccagc gtatcaccga aataagacat acgacgccag actacaaacg
     7561 aacccagcgg acccgcggca caggcgagca tgatcccggc taaccaaccg ggaaataata
     7621 attcaatcat gagcgatcat ttccccgacg caaaacaatt cgtccctgta aatcgtgacg
     7681 atgattatga tgatggcgat agatacccag ttgttcagca ccacgaggac caaacattga
     7741 aataaactcc ggatgcaggg aaacaacttc cggtgtgccg gaacaacaaa tgtggtgatt
     7801 caggcacagc acttcatcgg tttttgccat caccagatgc agatcgtgag aaaccattaa
     7861 aacgccacaa tccagttcac gacgcaactg gtcaataagg tcatataacg ccacttggcc
     7921 atttacatcc acgccttgag tgggttcatc cagcactaat aattgcgggc aatttaacaa
     7981 tgctcgcgct aatagtacac gctgcgtttc gccaccagag agcttttgca tcggtgcgtt
     8041 aatcaggtgc ccggcctgga cacgtttcag tgcaggcaaa atatcttctt tatgtgtacc
     8101 agggcgtaag cgtaaaaaac ggtttacggt cagtggcaac gtggtgtcga gatacagctt
     8161 ctgcggtaca tagccgatgc gcagttttcc gttgcgcttg ataacccctt catcgggtgt
     8221 taccagcccg agcactaccc gtaccagtgt cgacttacct gcgccatttg gcccaagtaa
     8281 agtcaaaatt tttccaggtt taagttccag cgacacatca gagaggacgc ggcgttggcc
     8341 aaaagaaacc gagacatttt ccagggaaac cagacttgtc atgttaattt tagtcttgca
     8401 gtagtcatga aatgttataa tatcacactt ctcatattca ttacgatgat tggtcgcatt
     8461 atgttacata aaaaaacgct tcttttcgca gcattatccg ccgctctctg gggaggtgca
     8521 acacaggccg cagatgccgc cgttgtcgct tcgcttaaac ccgttgggtt catcgcttct
     8581 gccattgctg atggtgtaac agaaacggag gttttactgc ctgacggcgc ttcagaacat
     8641 gattattcac tgcgtccatc ggatgtaaaa cgcttacaga acgcggactt agtcgtttgg
     8701 gttggcccgg agatggaagc gtttatgcaa aaaccggtaa gcaaattacc agaagcgaag
     8761 caggtaacga ttgcgcagct tgaagatgtg aaaccgctac tgatgaaaag tattcacggc
     8821 gatgatgatg atcacgacca cgcggaaaaa agtgacgaag atcaccatca cggcgatttc
     8881 aacatgcatc tttggctttc cccagagata gcgcgggcta cagcggttgc aatccatgga
     8941 aaattagtgg aacttatgcc gcaaagtcga gccaaacttg acgccaacct gaaggatttt
     9001 gaggcacaat tggcctcaac cgaaacgcag gttggtaacg agctcgcgcc gctcaagggg
     9061 aaaggttatt tcgtttttca cgatgcttac ggctatttcg aaaaacagtt cggactgaca
     9121 tcgcttggtc attttaccgt taaccctgag attcaacctg gcgcgcagcg tttacatgaa
     9181 ataagaacac agttggttga gcaaaaagca acctgcgttt ttgctgagcc acagttcagg
     9241 ccagcggtcg ttgagagcgt cgcacgaggg acatccgttc gtatgggaac gttggatccc
     9301 ctggggacga atatcaaact gggtaaaaca agctattcag aattcctgag tcaattagcc
     9361 aaccagtatg cgagctgcct gaaaggagat taatgaggaa gtgattacgt gcaacagata
     9421 gcccgctctg tcgccctggc gtttaataat ttaccgcgac cacaccgcgt tatgttgggg
     9481 tcgctcaccg ttcttactct ggccgtcgct gtctggcggc cctatgttta tcaccgtgat
     9541 gccacgccaa ttgtcaaaac cattgagctg gaacagaacg aaattcgttc gctcttacct
     9601 gaagccagtg agccgattga tcaagctgca caagaagatg aagccattcc ccaggacgaa
     9661 ctggatgaca aaatcgccgg tgaagcgggc gtgcatgaat atgttgtttc cactggcgat
     9721 acgctaagca gcattctcaa tcagtatggt attgatatgg gtgatatcac ccaacttgct
     9781 gctgccgaca aagaattgcg taacctgaaa atcggtcaac aactctcctg gacattaacc
     9841 gcggacggcg aactgcagcg cctcacctgg gaagtgtctc gtcgtgaaac ccgaacctat
     9901 gaccgtactg ccgctaacgg ttttaaaatg accagcgaaa tgcagcaagg agagtgggtt
     9961 aacaatctgc tgaaaggtac cgtcggaggg agctttgttg ccagcgccag aaacgccggt
    10021 ttaaccagcg ccgaagtgag cgcagtgatt aaagccatgc agtggcaaat ggatttccgc
    10081 aaactgaaaa aaggcgatga atttgcggta ttaatgtctc gagaaatgct tgatggtaaa
    10141 cgtgagcaaa gccagctgct gggcgtacgt ttgcgttcag aaggtaaaga ttattacgca
    10201 atccgcgctg aggatggcaa attctacgat cgtaacggta ctggtctggc gaaaggattc
    10261 ttgcgattcc cgacggcgaa acagttccgt atctcgtcta actttaaccc gcgtcgaact
    10321 aatccggtga caggtcgcgt tgcaccacac agaggtgttg atttcgccat gccacaaggt
    10381 acgccagtgc tttcagtggg tgacggtgaa gtggtggttg ccaaacgcag tggcgcagca
    10441 ggttattatg tggctattcg tcatggtcgc agctacacca cgcgttatat gcacttgcgc
    10501 aagattctgg tgaaaccggg acagaaggtg aaacgtggcg accgtatcgc gctttccggt
    10561 aataccggac gttcaactgg gccgcatctg cactatgaag tatggataaa ccagcaggcc
    10621 gtaaacccgc tgacggcaaa actgccgcgt accgaagggc tgaccggctc cgatcgtcgc
    10681 gaattcctgg cgcaggccaa agagattgtg ccgcagctac ggtttgatta attaacatcc
    10741 attcgcagcc ggtacgcagt cagtaccggc tttttttatt tggtgcgggg caagttgtgc
    10801 cgctacacta tcaccagatt gatttttgcc ttatccgaaa ctggaaaagc atggaaacga
    10861 aaaaaaataa tagcgaatac attcctgagt ttgataaatc ctttcgccac ccgcgctact
    10921 ggggagcatg gctgggtgta gcagcgatgg cgggtatcgc tttaacgccg ccaaagttcc
    10981 gtgatcccat tctggcacgg ctgggacgtt ttgccggacg actgggaaaa agctcacgcc
    11041 gtcgtgcgtt aatcaatctg tcgctctgct ttccagaacg tagtgaagct gaacgcgaag
    11101 cgattgttga tgagatgttt gccaccgcgc cgcaagcgat ggcaatgatg gctgagttgg
    11161 caatacgcgg gccggagaaa attcagccgc gcgttggctg gcaagggctg gagatcatcg
    11221 aagagatgcg gcgtaataac gagaaagtta tctttctggt gccgcacggt tgggccgtcg
    11281 atattcctgc catgctgatg gcctcgcaag ggcagaaaat ggcagcgatg ttccataatc
    11341 agggcaaccc ggtttttgat tatgtctgga acacggtgcg tcgtcgcttt ggcggtcgtc
    11401 tgcatgcgag aaatgacggt attaaaccat tcatccagtc ggtacgtcag gggtactggg
    11461 gatattattt acccgatcag gatcatggcc cagagcacag cgaatttgtg gatttctttg
    11521 ccacctataa agcgacgttg cccgcgattg gtcgtttgat gaaagtatgc cgtgcgcgcg
    11581 ttgtaccgct gtttccgatt tatgatggca agacgcatcg tctgacgatt caggtgcgcc
    11641 caccgatgga tgatctgtta gaggcggatg atcatacgat tgagcggcgg atgaatgaag
    11701 aagtcgagat ttttgttggt ccgcgaccag aacaatacac ctggatacta aaattgctga
    11761 aaactcgcaa accgggcgaa atccagccgt ataagcgcaa agatctttat cccatcaaat
    11821 aaaaaagcct ctcgcgagga gaggccttcg cctgatgata agttcaagtt tgcttcagaa
    11881 tattcgaaat ctgttgaact atcattgaac tataggccgg atgtggcgtt ttcgccgcat
    11941 ccggcaacgt acttactcta ccgttaaaat acgcgtggta ttagtagaac ccacggtact
    12001 catcacgtcg ccttgggtga caatcaccag gtcaccagac atcaagtaac ctttatcgcg
    12061 cagcagatta accgcttcgc tggcagctgc tacgccgtca ttagcgctat caaagggcac
    12121 cggcgtaacg ccacgataga gagcagtcag gttcagcgta cgttcatggc gcgacatggc
    12181 gaaaattggt agaccagagc tgatacggga ggtcatcagc gcggtacgac ccgattcggt
    12241 catggtaatg atcgccgtaa cgcccttcag gtggttagct gcgtacattg ccgacatggc
    12301 aatagcttct tccacattgt cgaactgaac gtccagacgg tgtttagaaa cgttgatgct
    12361 cgggattttt tccgcaccca ggcaaacgcg cgccatggct gcaacagttt ctgacggata
    12421 ctgcccagcg gcagtttctg cagacagcat cacagcgtca gtaccatcca gaacggcgtt
    12481 tgctacgtcc atgacttctg cacgcgtcgg catcgggtta gtaatcattg acaccatcat
    12541 ctgggtcgcc gtgattaccg ctcggtttag ctgacgcgca cgacggatca acgctttctg
    12601 aatgccgacc agttccgggt cgccaatttc cacaccgagg tcgccacgtg caaccattac
    12661 cacgtcagag gcgaggatga tgtcatccat tgcatcctgg ctgcaaacgg cttccgcacg
    12721 ttcaaccttg gcaacaattt tcgcatcaca tcctgcatcg cgtgccaggc gacgggcata
    12781 gttcagatct tcgccacagc gtgggaagga gacagccagg taatctacgc caatcaacgc
    12841 cgcagtctta atgtctgctt tgtctttttc ggtcagcgct tcagccgaca aaccgccgcc
    12901 aagtttgttg atacctttat tgttggagag gggaccaccg acggtgactt cggtgaacac
    12961 tttcatgccc tgaacttcca gtacttttaa ctggacgcga ccatcgtcca gcagcaggat
    13021 gtcaccaggc acgacgtcag taggcaggcc tttgtagtcg ataccgactt tttctttgtc
    13081 gccttcacct ttacccaggt tggcgtcgag caggaattta tccccaatat tgaggaaaac
    13141 tttgccttct ttaaaggtgg atacacggat tttgggcccc tggaggtcac ccagaatagc
    13201 cacatgacgc cccagttttg cggcaatctc acgaacttta tccgcgcgca ttttgtgatc
    13261 ttcaggcgag ccgtgagaaa agttcatacg tacaacgttg gcacccgccg cgataacttt
    13321 ttcaagatta ttatcgcgat ctgttgctgg gcctaacgtg gtaacgattt ttgttctgcg
    13381 aagccttttg gacatgtaat actccgttga ctgaaacaac caggtgttgc ttgaacatga
    13441 aatccgaatg aaataacgcc gcgatggcga taaattaatc tcattcggat gtcgtataag
    13501 attaggacag tgacagtcgc ttttagcgat cgtcacttaa attaagtaac tgcttatcaa
    13561 aacgcgattc cttcagcgct tccttgaccc gcttcaagtt atctctgaat tttgcaccgc
    13621 gtcgcaaagt aaatcctgtc gccagcacat ctatcacggt cagctgtgca agtcgagaaa
    13681 ccatgggcat ataaatgtca gtatcttccg gtacgtcgag ggtaattgcc agcgttgctt
    13741 cccgggcgag cggggtaccc gcagaggtga gggcaatcac catggcgtcg ttttcgcgtg
    13801 ccagctgcgc cagctcgacc agattttttg ttcttcctgt gtgagaaatc agcaccacca
    13861 cgtctccgtc gctacaattc atacaactca tgcgttgcag cacgatatca tcggagtaca
    13921 ccaccggaac attaaaacga aagaacttat tcatcgcatc gtgggcaacg gcggcagaag
    13981 agcctaatcc gaaaaaggcg attttttttg cctgagtgag caagtcgacg gcgcggttga
    14041 tggcagattt atccagtgaa tgacggacat gatcgagcgt tgccattgcg gactcaaata
    14101 ttttccccgt gtatgattca acgctgtcat cttcattgac gttgcgatta acatagggag
    14161 tgccattcgc cagactttgt gccagatgaa gtttaaaatc aggaaaaccg cgcgtgtcca
    14221 tgctgcgaca gaaacgattc accgtcggtt cgctaacatt ggcttccagc gccagagcag
    14281 caatactcga atggatcgcg ttatcgggcg aagccagaat gacctcggca actttgcgct
    14341 ctgatttgct caaatgttcc agctgagact ggattttttc cagcatattc atgatgtaaa
    14401 gagactcacg ggtaatgacg atttccgcac tgaaagaaat cgaaatgcag ttttgtcaga
    14461 tattacgcct gtgtgccgtg ttaatgacaa aagcagataa aaaagttgtt atttttttca
    14521 taacatgatc agtgtcagat ttttaccgaa tggaaaacga tgattttttt atcagttttg
    14581 ccgcgctttg cgcgcttttc ccgtaatcgc acgggtggat aagcgtttac agttttcgca
    14641 agctcgtaaa agcagtacag tgcaccgtaa gaaaattaca agtataccct ggcttaagta
    14701 ccgggttagt taacttaagg agaatgacat ggcggtaacg caaacagccc aggcctgtga
    14761 cctggtcatt ttcggcgcga aaggcgacct tgcgcgtcgt aaattgctgc cttccctgta
    14821 tcaactggaa aaagtcggtc agctcaaccc ggacacccgg attatcggcg tagggcgtgc
    14881 tgactgggat aaagcggcat ataccaaagt tgtccgcgag gcgctcgaaa ctttcatgaa
    14941 agaaaccatt gatgaaggtt tatgggacac cctgagtgca cgtctggatt tttgtaatct
    15001 cgatgtcaat gacactgctg cattcaaccg tctcggcgcg atgctggatc aaaaaaatcg
    15061 tatcactatt aactactttg ccatgccgcc cagcactttt ggcgcaattt gcaaagggct
    15121 tggcgaggca aaactgaatg ctaaaccggc acgcgtagtc atggagaaac cgctggggac
    15181 gtcgctggcg acctcgcagg aaatcaatga tcaggttggc gaatacttcg aggagtgcca
    15241 ggtttaccgt atcgaccact atcttggtaa agaaacggtg ctgaacctgt tggcgctgcg
    15301 ttttgctaac tccctgtttg tgaataactg ggacaatcgc accattgatc atgttgagat
    15361 taccgtggca gaagaagtgg ggatcgaagg gcgctggggc tattttgata aagccggtca
    15421 gatgcgtgac atgattcaga accacctgct acaaattctc tgcatgattg cgatgtctcc
    15481 gccatctgac ctgagcgcag acagcatccg cgatgaaaaa gtgaaagtac tgaagtccct
    15541 gcgccgcatc gaccgctcca acgtacgcga aaaaaccgta cgcgggcaat atactgcggg
    15601 cttcgcccag ggcaaaaaag tgccgggata tctggaagaa gagggcgcga acaaaagcag
    15661 caatacagaa accttcgtgg cgatccgcgt cgacattgat aactggcgct gggccggtgt
    15721 gccattctac ctgcgtactg gtaaacgtct gccgaccaaa tgttctgaag tcgtggtcta
    15781 tttcaaaaca cctgaactga atctgtttaa agagtcgtgg caggatctgc cgcagaataa
    15841 actgactatc cgtctgcaac ctgatgaagg cgtggatatc caggtactga ataaagttcc
    15901 tggccttgac cacaaacata acctgcaaat caccaagctg gatctgagct attcagaaac
    15961 ctttaatcag acgcatctgg cggatgccta tgaacgtttg ctgctggaaa ccatgcgtgg
    16021 tattcaggca ctgtttgtac gtcgcgacga agtggaagaa gcctggaaat gggtagactc
    16081 cattactgag gcgtgggcga tggacaatga tgcgccgaaa ccgtatcagg ccggaacctg
    16141 gggacccgtt gcctcggtgg cgatgattac ccgtgatggt cgttcctgga atgagtttga
    16201 gtaatatcag cgcttatcct ttatggttat tttaccggta acatgatctt gcacagattg
    16261 tagaacaatt tttacacttt caggcctcgt gcggattcac ccacgaggct ttttttatta
    16321 cactgactga aacgtttttg ccctatgagc tccggttaca ggcgtttcac tcataaatcc
    16381 tctgaatgaa acgcgttgtg aatcatcctg ctctgacaac tcaatttcag gagcctttat
    16441 gaatccacaa ttgttacgcg taacaaatcg aatcattgaa cgttcgcgcg agactcgctc
    16501 tgcttatctc gcccggatag aacaagcgaa aacgtcgacc gttcatcgtt cgcagttggc
    16561 atgcggtaac ctggcacacg gtttcgctgc ctgccagcca gaaaacaaag cctctttgaa
    16621 aagcatgttg cgtaacaata tcgccatcat cacctcctat aacgacatgc tctccgcgca
    16681 ccagccttat gaacactatc cagaaatcat tcgtaaagcc ctgcatgaag cgaatgcggt
    16741 tggtcaggtt gcgggcggtg ttccggcgat gtgtgatggt gtcacccagg ggcaggatgg
    16801 aatggaattg tcgctgctaa gccgcgaagt gatagcgatg tctgcggcgg tggggctgtc
    16861 ccataacatg tttgatggtg ctctgttcct cggtgtgtgc gacaagattg tcccgggtct
    16921 gacgatggca gccctgtcgt ttggtcattt gcctgcggtg tttgtgccgt ctggaccgat
    16981 ggcaagcggt ttgccaaata aagaaaaagt gcgtattcgc cagctttatg ccgaaggtaa
    17041 agtggaccgc atggccttac tggagtcaga agccgcgtct taccatgcgc cgggaacatg
    17101 tactttctac ggtactgcca acaccaacca gatggtggtg gaatttatgg ggatgcagtt
    17161 gccaggctct tcttttgttc atccggattc tccgctgcgc gatgctttga ccgctgccgc
    17221 tgcgcgtcag gttacacgca tgaccggtaa tggtaatgaa tggatgccga tcggtaagat
    17281 gatcgatgag aaagtggtgg tgaacggtat cgttgcactg ctggcgaccg gtggttccac
    17341 taaccacacc atgcacctgg tggcgatggc gcgcgcggcc ggtattcaga ttaactggga
    17401 tgacttctct gacctttctg atgttgtacc gctgatggca cgtctctacc cgaacggtcc
    17461 ggccgatatt aaccacttcc aggcggcagg tggcgtaccg gttctggtgc gtgaactgct
    17521 caaagcaggc ctgctgcatg aagatgtcaa tacggtggca ggctttggtc tgtctcgtta
    17581 tacccttgaa ccatggctga ataatggtga actggactgg cgggaagggg cggaaaaatc
    17641 actcgacagc aatgtgatcg cttccttcga acaacctttc tctcatcatg gtgggacaaa
    17701 agtgttaagc ggtaacctgg gccgtgcggt tatgaaaacc tctgccgtgc cggttgagaa
    17761 ccaggtgatt gaagcgccag cggttgtttt tgaaagccag catgacgtta tgccggcctt
    17821 tgaagcgggt ttgctggacc gcgattgtgt cgttgttgtc cgtcatcagg ggccaaaagc
    17881 gaacggaatg ccagaattac ataaactcat gccgccactt ggtgtattat tggaccggtg
    17941 tttcaaaatt gcgttagtta ccgatggacg actctccggc gcttcaggta aagtgccgtc
    18001 agctatccac gtaacaccag aagcctacga tggcgggctg ctggcaaaag tgcgcgacgg
    18061 ggacatcatt cgtgtgaatg gacagacagg cgaactgaca ctgctggtag acgaagcgga
    18121 actggctgct cgcgaaccgc acattcctga cctgagcgcg tcacgcgtgg gaacaggacg
    18181 tgaattattc agcgccttgc gtgaaaaact gtccggtgcc gaacagggcg caacctgtat
    18241 cactttttaa gacgacaaat ttgtaatcag gcgagagaaa actctgatga aaaactggaa
    18301 aacaagtgca gaatcaatcc tgaccaccgg cccggttgta ccggttatcg tggtaaaaaa
    18361 actggaacac gcggtgccga tggcaaaagc gttggttgct ggtggggtgc gcgttctgga
    18421 agtgactctg cgtaccgagt gtgcagttga cgctatccgt gctatcgcca aagaagtgcc
    18481 tgaagcgatt gtgggtgccg gtacggtgct gaatccacag cagctggcag aagtcactga
    18541 agcgggtgca cagttcgcaa ttagcccggg tctgaccgag ccgctgctga aagctgctac
    18601 cgaagggact attcctctga ttccggggat cagcactgtt tccgaactga tgctgggtat
    18661 ggactacggt ttgaaagagt tcaaattctt cccggctgaa gctaacggcg gcgtgaaagc
    18721 cctgcaggcg atcgcgggtc cgttctccca ggtccgtttc tgcccgacgg gtggtatttc
    18781 tccggctaac taccgtgact acctggcact gaaaagcgtg ctgtgcatcg gtggttcctg
    18841 gctggttccg gcagatgcgc tggaagcggg cgattacgac cgcattacta agctggcgcg
    18901 tgaagctgta gaaggcgcta agctgtaaaa gtcaaaatgc ccgatctagg atcgggcatt
    18961 tttgtagcgt ttttttgccg ggtttaaccc tgtactttta cctgtccggc ggcgtgcttc
    19021 gcgcgttcaa tggcgtcaac aacactctct gaagtagcca gtaccacccc cagatgacgg
    19081 ctgccatcaa tttccggctt accaaataaa cgaatctgca aatctgcgcc tacggcattc
    19141 tgcacattat caaacgtgac attctgactg gtcagttgtg gcagaataac ggcagaagct
    19201 gcaggaccat actgacggat cccgccaaca ggaagtccga ggaaggcacg tacatgcagg
    19261 gcaaactctg agagatcttg agaaattaac gtcaccatcc cggtatcatg tggacgaggg
    19321 gagacctcac tgaaaatcac ctcatcacca cagacaaaca gctcgacacc aaacagacca
    19381 taaccgccca gtgccagcac cactttacgg gcaatctcct gcgcacgttc aagggcaagc
    19441 gggctcattt gctgtggttg ccaggattca cggtagtcgc catcttcctg gcgatgacct
    19501 actggtgcac agaaatggac gccatccacc gcgctgacgg ttagcagggt aatttcgaag
    19561 tcaaacttaa cgacgccttc aacaattacg cggcccgctc cggcgcgacc gccttgctga
    19621 gcgtattccc atgcctgagc aagttgctct gcagagcgaa taaacgtctg ccccttgccg
    19681 gaagagctca tcaccggttt tacaatgcag ggatagccaa tggcggcaac cgcctcgcgg
    19741 aaaaggcttt tgctatcggc aaaacgataa gtggaagtgg gcagctgcag ctcttctgcc
    19801 gccaggcgac ggataccctc gcgattcatc gttaatttcg ttgcgcgagc gcaggggaca
    19861 acattcagtc cttcctcttc aagttggatc agcatatcgg tggcaatagc ttcgatctcc
    19921 ggcacgatat aatgtggttt ttccagttca accacacggc gtaatgcatc accatcaagc
    19981 atattaatga catgggagcg atgcgcgaca tgcatggctg gtgcgtcggc atagcgatcg
    20041 acggcaatca cctctacgcc gagacgctga cactcgattg ccacttcttt acccagttca
    20101 ccggagccta ataacatcac gcgagttgct gccggacgca gcgcagtgcc taataacgtc
    20161 ataacgaata tcccctgatt aattccgcgc gcagtataaa cgaaaacgtt tgcgtgtgtc
    20221 tttattgctg atgttgattt ctcaaccgaa aagaaatata ctgtataaaa tctcagttat
    20281 tatgagaggc tgcaaatggc ggttgaagtt aaatacgtag tcattcgtga gggcgaagag
    20341 aaaatgtcgt ttaccagcaa aaaggaagcc gatgcttatg acaaaatgct tgatacggcg
    20401 gatctgcttg acacctagct gacaaattct ccagtgcaaa tggaagacga gcaacgtgaa
    20461 gccctttcgc tatggctggc agaacaaaaa gaggtgctga gcaccattct gaaaaccggc
    20521 aaattaccgt ccccacaggt cgtcggtgct gaatccgaag aggaagatgc ctcacacgcg
    20581 gcctgaagca acttgcgctc ctgacaattt gtaccatgct ttctcctgaa tcattgtgct
    20641 atggagaaaa acatgaaaaa aagaggggcg tttttagggc ggttgttggt ttctgcctgc
    20701 gcatcagttt tcgctgccaa taatgaaacc agcaagtcgg tcactttccc aaagtgtgaa
    20761 ggtctggatg ctgccggaat tgccgcgagc gtaaaacgtg attatcaaca aaatcgcgtg
    20821 gcgcgctggg cagatgatca aaaaattgtc ggtcaggccg atcccgtggc ctgggtcagt
    20881 ttgcaggaca ttcagggtaa agatgataaa tggtcagtac cgctaaccgt gcgtggtaaa
    20941 agtgccgata ttcattacca ggtctgcgtg gattgcaaag cgggaatggc ggaatatcag
    21001 cggcgttaat ggcttctgat aattcgattg accgttggcg gatgcggcgc gagtgccttg
    21061 tccgtcccag aacgcaccac aatcgtaggc ctgagaagac gcaacagcgt cgcatcgggc
    21121 aatcagcatc aaacaatgcc actcaaaacg acaaactact ttcacctaaa atacccttat
    21181 cacctgatat acagtaaata ccttacctgg cgcgaatttc tttgaataac ggcctaccgc
    21241 cccgcttatt tgcgcatctt ttgtctcgat atacattttc tgacatcctc atccgctatg
    21301 ctacaggcac taaaaatggt tattgaggag caaatatggc taactggtta aatcaactgc
    21361 aatccctgct tgggcaaagc agttcttcta cctcctcgtc tgcggatcag ggattgggca
    21421 aactgttagt gtcaggcgca ttaggcggac tggctgggct gctggtcgca aataaatcag
    21481 cacgtaaact tcttacgaaa tatggcacca acgcgttact ggttggcggc ggagcggtag
    21541 cgggtacggt gctgtggaat aaatacaaag ataaaattcg cgcggcgcat caggacgaac
    21601 cgcagtttgg cgcgcaaagt acgccgctgg atgagcgtac ggaacgtttg atccttgcgc
    21661 tggtctttgc cgctaaaagt gatggtcata ttgatgccaa tgaacgtgcg gcaatcgacc
    21721 agcaattgcg tgaagccggc gtggaagagc aggggcgtgt actcattgag caggcaatcg
    21781 aacaaccgct ggatccacaa cgcctggcta ccggggtccg caatgaagaa gaggcgctgg
    21841 aaatctattt cctgagctgc gcggctattg atattgacca ttttatggaa cgcagttacc
    21901 tgaatgcact gggtgacgcg ctgaaaatcc cccaggatgt tcgtgaaggc attgaacgcg
    21961 atctcgaaca gcaaaaacgc actttggcgg aataattccg ctctctggat agcctgaagt
    22021 tgactatttc gggctatcca gaaccgcaca gccatttgaa caggctgatt ttctctgtct
    22081 gcataacatt cgatatccgc gccattacct gtcatcatct aagcaatgac gaccctgttt
    22141 cgcttgcatc cccggtgagt tttgccaccc ttataagatg tttccaacca gaaagaacaa
    22201 taacatgcta ccaaaagccg cccgcattcc ccacgccatg acgcttcatg gcgatacgcg
    22261 catcgataat tactactggc tgcgggacga tacgcgttct cagccaaaag tcctggacta
    22321 cctgcaacaa gaaaatagtt acggtcatcg ggtgatggcc tcacaacaag ccttgcagga
    22381 tcgcatctta aaggaaatca tcgaccgcat tccgcaaaga gaagtttctg cgccctacat
    22441 aaaaaatggc taccgctatc ggcatattta tgaaccaggc tgtgaatatg ctatctacca
    22501 gcgtcaatcg gcattcagcg aagagtggga tgagtgggaa acattgctcg atgccaacaa
    22561 gcgcgcagct catagtgagt tttattcgat gggcggaatg gcgattacgc ccgataacac
    22621 cattatggcg ctggcagaag attttctttc ccgacgccag tacggcattc gttttcgtaa
    22681 tctggaaaca ggtaactggt acccggaact gctggataac gttgaaccca gctttgtctg
    22741 ggcaaatgac tcctggactt tctactatgt tcgcaagcat ccggtgacgc tgctgcctta
    22801 tcaggtctgg cgtcacgcca tcggtacgcc agcatcgcaa gatacactga tctacgaaga
    22861 aaaagacgat acctattatg tcagcctgca taaaacgacg tcgaagcact atgtcgtcat
    22921 tcatctggcc agcgccacca ccagtgaagt tcgcctgctg gacgcggaaa tggccgatgc
    22981 cgagccgttt gtttttctgc cgcgccgcaa agatcacgaa tacagccttg atcactacca
    23041 gcatcgtttt tatctgcgtt ccaatcgcca cggcaaaaac tttggcttat accgtacccg
    23101 tatgcgtgat gagcaacagt gggaagagtt aattccgcca cgcgaaaaca tcatgctgga
    23161 agggtttacg ctgtttaccg actggctggt ggttgaagag cgtcagcgcg ggttaaccag
    23221 tttgcgccaa attaaccgca agacccggga agtcattggc attgcctttg atgatccggc
    23281 ctatgtgacc tggattgcct acaatccaga acctgaaacc gcgcgattgc gttatggtta
    23341 ttcttccatg accacaccag acactttgtt tgaactggat atggataccg gtgagcgtcg
    23401 tgtattaaaa caaacagaag tgcctggttt tgatgcggcg aattaccgca gtgaacacct
    23461 gtggatagtc gcccgtgatg gcgtcgaagt tccggtttcg ctggtctatc atcgcaaaca
    23521 ttttcgcaaa ggacacaacc cgttgctggt gtatggctat ggttcttacg gcgcaagtat
    23581 tgatgccgat ttcagtttta gccgcttgag tttgttagat cgtggctttg tctacgccat
    23641 tgtccatgtt cgcggcggtg gtgagctggg gcaacaatgg tacgaagacg gtaaatttct
    23701 gaagaagaaa aatacgttta atgattatct tgatgcctgc gatgcattgt taaaactggg
    23761 ctatggctct ccttcgctct gttatgcgat gggcggaagt gcggggggca tgttgatggg
    23821 cgttgcaatt aatcaacgcc cggaattatt ccacggcgtt atcgcccagg taccgtttgt
    23881 tgatgttgta acaacgatgc ttgatgaatc aattcctctt accactggtg agtttgaaga
    23941 gtggggaaat ccgcaggatc cgcaatatta cgagtacatg aaaagctaca gcccgtatga
    24001 caacgtcacc gcactggctt atccgcattt actggtaacg accggtttac acgattctca
    24061 ggtgcaatat tgggaaccgg caaaatgggt cgctaaattg cgcgagctga aaaccgatga
    24121 ccatctttta ttgctctgta ccgacatgga ctcaggccat ggcggtaaat ctggccgctt
    24181 taaatcgtac gaaggcgtag cgatggaata tgcttttctg gtcgcgctgg cgcagggaac
    24241 attacccgct acgcctgcgg attaagtatt ttccagataa tgtttcagtg ttaaacgcag
    24301 ctccgggctc atgctgtcca ggttattaaa taaccagcgc agatagcccg gatagcgttc
    24361 ggcaacgtcg gaaaccgctt tgccacggta tttgccaaag gtgaaggttg tcatcaacga
    24421 cggacgtccg gtgatatcgg ccatctgttc tgccgtccag ccggaggtgt tcataatatc
    24481 gataagcaac gcggcggtga tataacagtc atacaacgcg cggtgatgat gcaggcccag
    24541 cggtgtctgt acattgagct tgcgtgtttt atataacgcc atattgctgt acttgagccc
    24601 tggccacaaa cgacgggcca gtttcatagt gcaaatccac tcaccgggca tctcaggcag
    24661 tacgcggcgg tcaaagctgg cgttgtgcgc gacataccat tcactaccgt aatagtgtgg
    24721 gatcacatct tcaatccacg gtttatcggc gaccatggct tcggtgatgc gatgaatcgc
    24781 catcgcttgt ggactaatag gacgatcggg gcgcaccagg aggctcatgg ggttgacgat
    24841 ttttccgtca atgacatcaa cagaggcaat ctcaacgatc cctccctgca aaccgcaggt
    24901 ttctgtatcg ataatgcgca acatggcatg atccaggccg aaaaagccta gcgtaatgga
    24961 atgatatcgc cttgccaacc ctgtgatgaa cgctgaccga ctaacaataa tgaaccacga
    25021 tcggcgcgaa cgatgagacg accatattca tcccatagtg cactgttacc acgggcattt
    25081 gccatcagca cggcaatcga gtactgatgt gaaaaaaatt gtaagcgacg ggccgacgcc
    25141 agcaggtcgg gttctcccag acattgccct gtcgtaaaca gtgaacaggt cgggtccata
    25201 tccatacctt gcggttgttc atcgaccacc gtgatggttc tggaacgacg gcccagacat
    25261 gcaccatgac tctgatggta gttcccaggc gtttttcgcc acggtgcgaa caccgcaatt
    25321 ccacgaataa agcgatcgtt atattcaacg ggaaggccgg caatgatggt cattcgctag
    25381 gtcgttgcgg cataacaaag cgggtctaac agtgaaagat cgggtggggc aggaagggca
    25441 cgtcgcgaat aatcacaccc cagtaaagaa agcgatggaa aaactaacag ctgacattgt
    25501 tgcctggcgg cggccctcac aaactccaga tgatgagcta cctgttcggt gagtgaggtt
    25561 ttacgaggct catattgcgc tgctgcaact ttccagaacg acataatgac ttccttttca
    25621 aaatgactgc aaattatggg ggcggtttag aaaatactca taatttagca ggcgttatgt
    25681 aagaaagtgt aactctgtat aagttttatt taagtttggg ctcgtaaggt aaacgtgaca
    25741 gattgaccga agccaaacgg tgggcaataa gccgctcgcg aaaccagctg cgcaaatgtt
    25801 ctggctgttc acgttcaacc gcttcagcga tcaccggcat attgtagcgt tctttaaaag
    25861 ccaccccggc cgccgccaaa tcgacattca ctttatccat ttctgtttga tccagtttag
    25921 ccagattctt cagcattctt aatctcctca tcattcgcgg gcaaaagtta ctaagagtga
    25981 gcctggatgg caagccggaa ttgatgatcc tcgactcaga cattagacaa ggttatgctc
    26041 tgtaaaaaag ttattacaaa aaggaataga taatatggct tcaactgcac gctcccttcg
    26101 ctacgcgctg gcaatcctga ccacttcgtt ggtgacacct tccgtctggg cacatgcgca
    26161 tttaacgcat cagtatcctg cggcaaacgc gcaagtgaca gctgccccgc aggcaatcac
    26221 tttaaacttc tcggaaggcg ttgaaacagg attcagtggt gcaaaaatca cggggccaaa
    26281 aaacgaaaat attaaaacat taccagcgaa gcggaatgag caggaccaaa agcaactgat
    26341 tgttccactg gccgattcgc tgaaacccgg aacctacacc gttgactggc atgttgtttc
    26401 ggtggacggg cataaaacga aaggacacta cacctttagc gtgaaataaa tcatgctggc
    26461 gtttacctgg atcgcgctgc gatttatcca tttcacctcg ttgatgctgg tctttggctt
    26521 tgcaatgtat ggtgcctggc tggccccctt aatgattcgt cgcttgctga cgaagcgttc
    26581 tctgcgttta caacagcacg ctgccgtctg gagtttgatc agtgccactg caatgctcgc
    26641 cgttcagggc gggttgatgg gaacgggatg gacagatgta ttttcgccaa acatttggca
    26701 ggctgttttg caaacgcagt ttggtggcgt ctggttatgg caaattgttc tcgccctcgt
    26761 aacgttgatc gtcgccctta tgcaaccgcg gaatatgcca cgtttgctgt ttatgctcac
    26821 taccgcgcaa tttattctgc tggcgggagt ggggtatgcg acgctgaatg aaggggtaac
    26881 agcgaaaatc catcagacta atcacgcgat tcacctgatt tgtgcggcgg cgtggtttgg
    26941 gggattactg ccggtgctct ggtgtatgca actcatcaaa ggccgttggc gacactaggc
    27001 tattcaggcg ctgatgcgtt tttcctggtg cgggcatttt gcggtgatcg gcgtactggc
    27061 aagcggcgtg cttaatgcat tgttgattac cggatttccc cccacgctta cgacttactg
    27121 gggccaactc ttattgctca aagccatcct ggtgatgatt atggtggtca ttgcgctggc
    27181 taatcgttat gttcttgtac cgcgcatgag gcaggatgaa gatcgtgcgg caccctggtt
    27241 tgtgtggatg actaaactcg aatgggcgat aggtgcggta gtgctggtga tcatcagcct
    27301 gcttgcgact ctcgaacctt tttgatggat tacgaaaatg atgaaaaaaa gtattctggc
    27361 gtttctgtta ctcaccagtt ctgcagcggc gctggcggct ccacaggtga taaccgtgag
    27421 tcgttttgaa gtgggtaaag acaaatgggc gtttaatcgc gaagaggtaa tgctgacttg
    27481 ccgaccgggt aatgctttgt atgtcatcaa cccaagtacc ctcgtgcagt atcctttaaa
    27541 cgatatcgca caaaaggaag ttgccagtgg gaagaccaat gcccaaccca tttcggtgat
    27601 tcagattgat gatcctaaca atcccggcga aaaaatgagt ctggcaccgt ttatagaacg
    27661 agctgaaaaa ctctgttaat tacctaaaat agccttttga tttccaataa aaaaaccgcc
    27721 tcagttcttt caccagaacg ggcggttttt aacatttaag ctgatgagca ccgcgctttt
    27781 tattgaccat tttgcacgca aactggaaaa cctggcgtcg tcatctattc ttaaagggca
    27841 aggcaactaa gcctgcatta atgccaactt ttagcgcacg gctctctccc aagagccatt
    27901 tccctggacc gaatacagga atcgtattcg gtcttttttt aattgtattt aaaatcaatt
    27961 agttgcaaac gtctccccga aattccccga aatttactcg aatttctgta ttccggtctt
    28021 ttttggttat atcacaacca aaatacattt aacaatccat ttacgttaaa atcagagcag
    28081 taagtacgtt ttttctctct catcaagata catttttgtt gtcttctccg atgtgtggcc
    28141 aagtagacgc tgagcaaatt cttctccaca tatttctttg tacaatcgtc cagccaggct
    28201 tctgatttcg tgaaaagttg gtgggttttc actgaacagt gatacctgtt aattttctgg
    28261 ctgcgacaaa tttctttgtc aggccgtccg ggtgaatgct gccgtcaggg ctgttttttc
    28321 taatcccggc actgattaga taatcccccc tacttaccat gcggcactgt tcaactactg
    28381 caccaagtcg taaaccagcg acaggaaggc tgagtgacag ggggatagca atcatcattc
    28441 ctgtcttaat ttgcctgatg tggagacgat catcataaat atcactgaac cgcatattcg
    28501 ttatgtcttc gcgacgttgt cctgttacaa gggctaagtc catagctaat gggaaccatg
    28561 ccggaagttg atctgctgcc tccctgatgc agttgtatgt ctttagtttc agccgttctc
    28621 ttgtaactac tattttcggt gctcttgttg gcgttactgg attttgagat atacgtcctt
    28681 caacaatggc ttcgcggaac atatcagata acacagaacg cattgatcct gccatcgtgt
    28741 ttttccctcc ttcaatctac aaatcaagaa actcggcgat atggcgagtg gttatttctg
    28801 tcagtaaaac ccctcctaat ttttctttta ttgtctccag ttgatttacc cgagttttat
    28861 aagtatttct ggacgctttt ctccttataa gaatcgtttt gtaacgttca atccagcctg
    28921 ccatagtaaa tgagtcgacc cctttaagct tttcaattag ggcagcagga gagtagtttt
    28981 tgtatatata atgatttgct tcaattgcct gcgctactgc atctcttctt gaaattttac
    29041 ctagtgtaaa ttcttctttc gtcagagggt tgcgccagta atatgctttg tctctccttc
    29101 gatatgttaa gtttctaggc aaattgggat cgtatttttt ccgctgcatg ttttaacttc
    29161 tccattaacg gactgtctct cccttgtcgc ccattaggct gatggtgtgt tatatcggta
    29221 tcaaccttat ttgggttgat atagaaagcc tccggaacca ccctgtaact cctcccgtgt
    29281 agttcaggtg caggataaat gtttccattc cttgcccatc gtctcagcgt tgatattgat
    29341 ggtgggttat ccggatatct gagttttccc cacgttttga gtgtcacaag attcattgcc
    29401 atacctctca cgatatgacc gccagtaaat atacagaata ctggcgggtg tggttgattt
    29461 ttaata
//
LOCUS       DAJTSI010000039        29351 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000039 DAJTSI010000000
VERSION     DAJTSI010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.039"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="OO327_001529"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="OO327_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR6018113.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="OO327_001530"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="OO327_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR6018114.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="OO327_001531"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="OO327_001531"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR6018115.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="OO327_001532"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="OO327_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR6018116.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="OO327_001533"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="OO327_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR6018117.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="OO327_001534"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="OO327_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR6018118.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="OO327_001535"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="OO327_001535"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR6018119.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8747..9127
                     /gene="atpI"
                     /locus_tag="OO327_001536"
     CDS             8747..9127
                     /gene="atpI"
                     /locus_tag="OO327_001536"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR6018120.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9136..9951
                     /gene="atpB"
                     /locus_tag="OO327_001537"
     CDS             9136..9951
                     /gene="atpB"
                     /locus_tag="OO327_001537"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR6018121.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9998..10237
                     /gene="atpE"
                     /locus_tag="OO327_001538"
     CDS             9998..10237
                     /gene="atpE"
                     /locus_tag="OO327_001538"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR6018122.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10299..10769
                     /gene="atpF"
                     /locus_tag="OO327_001539"
     CDS             10299..10769
                     /gene="atpF"
                     /locus_tag="OO327_001539"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR6018123.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10784..11317
                     /gene="atpH"
                     /locus_tag="OO327_001540"
     CDS             10784..11317
                     /gene="atpH"
                     /locus_tag="OO327_001540"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR6018124.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRSGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11330..12871
                     /gene="atpA"
                     /locus_tag="OO327_001541"
     CDS             11330..12871
                     /gene="atpA"
                     /locus_tag="OO327_001541"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR6018125.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12922..13785
                     /gene="atpG"
                     /locus_tag="OO327_001542"
     CDS             12922..13785
                     /gene="atpG"
                     /locus_tag="OO327_001542"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR6018126.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13812..15194
                     /gene="atpD"
                     /locus_tag="OO327_001543"
     CDS             13812..15194
                     /gene="atpD"
                     /locus_tag="OO327_001543"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR6018127.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15215..15634
                     /gene="atpC"
                     /locus_tag="OO327_001544"
     CDS             15215..15634
                     /gene="atpC"
                     /locus_tag="OO327_001544"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR6018128.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15985..17355
                     /gene="glmU"
                     /locus_tag="OO327_001545"
     CDS             15985..17355
                     /gene="glmU"
                     /locus_tag="OO327_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR6018129.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17517..19346
                     /gene="glmS"
                     /locus_tag="OO327_001546"
     CDS             17517..19346
                     /gene="glmS"
                     /locus_tag="OO327_001546"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR6018130.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            19508..20329
                     /locus_tag="OO327_001547"
     CDS             19508..20329
                     /locus_tag="OO327_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HCR6018131.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            20316..22424
                     /locus_tag="OO327_001548"
     CDS             20316..22424
                     /locus_tag="OO327_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR6018132.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            22421..24088
                     /locus_tag="OO327_001549"
     CDS             22421..24088
                     /locus_tag="OO327_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6018133.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            24091..25617
                     /locus_tag="OO327_001550"
                     /pseudo
     CDS             24091..25617
                     /locus_tag="OO327_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
     gene            25618..27234
                     /locus_tag="OO327_001551"
     CDS             25618..27234
                     /locus_tag="OO327_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR6018134.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALELSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(27290..27442)
                     /locus_tag="OO327_001552"
                     /pseudo
     CDS             complement(27290..27442)
                     /locus_tag="OO327_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(27465..27842)
                     /locus_tag="OO327_001553"
     CDS             complement(27465..27842)
                     /locus_tag="OO327_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018135.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            complement(28252..28623)
                     /locus_tag="OO327_001554"
     CDS             complement(28252..28623)
                     /locus_tag="OO327_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018136.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(28684..29181)
                     /locus_tag="OO327_001555"
     CDS             complement(28684..29181)
                     /locus_tag="OO327_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018137.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(29210..29351)
                     /locus_tag="OO327_001556"
                     /pseudo
     CDS             complement(29210..29351)
                     /locus_tag="OO327_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase"
BASE COUNT         7573 a         6856 c         7552 g         7370 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttattctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccgagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgttcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggca caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaggtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cattagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gagtggtttt tgttgtgtaa atgttattta ttaaaacagt
     8401 atctgttttt agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa
     8461 tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta
     8521 cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc
     8581 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
     8641 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
     8701 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
     8761 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag
     8821 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
     8881 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc aggcgcatac
     8941 accagcgaaa ggccgggtgg cttggacatt cgcatttggc gaagctttca aagttctggc
     9001 gatgttggtg ttactggtgg tggcgttggc ggttttaaag gcggtattct tgccgctgat
     9061 cgttacgtgg gttttggtgc tggtggttca gatactggca ccggctgtaa ttaacaacaa
     9121 agggtaaaag gcatcatggc ttcagaaaat atgacgccgc aggattacat aggacaccac
     9181 ctgaataacc ttcagctgga cctgcgtaca ttctcgctgg tggatccaca aaacccccca
     9241 gccaccttct ggacaatcaa tattgactcc atgttcttct cggtggtgct gggtctgttg
     9301 ttcctggttt tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc aggtaagttt
     9361 cagaccgcga ttgagctggt gatcggcttt gttaatggta gcgtgaaaga catgtaccat
     9421 ggcaaaagca agctgattgc tccgctggcc ctgacgatct tcgtctgggt attcctgatg
     9481 aatctgatgg atttactgcc tatcgacttg ctgccgtaca ttgctgaaca tgtactgggt
     9541 ctgcctgcac tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc tatggcactg
     9601 ggcgtattta tcctgattct gttctacaac atcaaaatga aaggcatcgg cggcttcacg
     9661 aaagagttga cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa cttaatcctt
     9721 gaaggggtaa gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt cggtaacatg
     9781 tatgccggtg agctgatttt cattctgatt gctggtctgt tgccgtggtg gtcacagtgg
     9841 atcctgaatg tgccgtgggc cattttccac atcctgatca ttacgctgca agccttcatc
     9901 ttcatggttc tgacgatcgt ctatctgtcg atggcgtctg aagaacatta atttaccaac
     9961 actactacgt tttaactgaa acaaactgga gactgtcatg gaaaacctga atatggatct
    10021 gctgtacatg gctgccgctg tgatgatggg tctggcggca atcggtgctg cgatcggtat
    10081 cggcatcctc gggggtaaat tcctggaagg cgcagcgcgt caacctgatc tgattcctct
    10141 gctgcgtact cagttcttta tcgttatggg tctggtggat gctatcccga tgatcgctgt
    10201 aggtctgggt ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt tatttaaaga
    10261 gcaatatcag aacgttaact aaatagaggc attgtgctgt gaatcttaac gcaacaatcc
    10321 tcggccaggc catcgcgttt gtcctgttcg ttctgttctg catgaagtac gtatggccgc
    10381 cattaatggc agccatcgaa aaacgtcaaa aagaaattgc tgacggcctt gcttccgcag
    10441 aacgagcaca taaggacctt gaccttgcaa aggccagcgc gaccgaccag ctgaaaaaag
    10501 cgaaagcgga agcccaggta atcatcgagc aggcgaacaa acgccgctcg cagattctgg
    10561 acgaagcgaa agctgaggca gaacaggaac gtactaaaat cgtggcccag gcgcaggcag
    10621 aaattgaagc cgagcgtaaa cgtgcccgtg aagagctgcg taagcaagtt gctatcctgg
    10681 ctgttgctgg cgccgagaag atcatcgaac gttccgtgga tgaagctgct aacagcgaca
    10741 tcgtggataa acttgtcgct gaactgtaag gagggagggg ctgatgtctg aatttattac
    10801 ggtagctcgc ccctacgcca aagcagcttt tgactttgcc gtcgaacacc aaagtgtaga
    10861 acgctggcag gacatgctga cgtttgccgc cgaggtaacc aaaaacgaac aaatggcaga
    10921 gcttctctct ggcgcgcttg cgccagaaac gctcgccgag tcgtttatcg cagtttgtgg
    10981 tgagcaactg gacgaaaacg gtcagaacct gattcgggta atggctgaaa atggtcgtct
    11041 taacgcgctc ccggatgttc tggagcagtt tattcacctt cgtgccgtga gtgaggctac
    11101 cgctgaggta gacgtcattt ccgctgccgc actgagtgaa caacagctcg cgaaaatttc
    11161 tgctgcgatg gaaaaacgtc tgtcacgcaa agttaagctg aattgcaaaa tcgataagtc
    11221 tgtaatggct ggcgttatca tccgatcggg tgatatggtc attgatggca gcgtacgcgg
    11281 tcgtcttgag cgccttgcag acgtcttgca gtcttaaggg gactggagca tgcaactgaa
    11341 ttccaccgaa atcagcgaac tgatcaagca gcgcattgct cagttcaatg ttgtgagtga
    11401 agctcacaac gaaggtacta ttgtttctgt aagtgacggt gttatccgca ttcacggcct
    11461 ggccgattgt atgcagggtg aaatgatctc cctgccgggt aaccgttacg ctatcgcact
    11521 gaacctcgag cgcgactctg taggtgcggt tgttatgggt ccgtacgctg accttgccga
    11581 aggcatgaaa gttaagtgta ctggccgtat cctggaagtt ccggttggcc gtggcctgct
    11641 gggccgtgtg gttaacactc tgggtgcacc aatcgacggt aaaggtccgc tggatcacga
    11701 cggcttctct gctgtagaag caatcgctcc gggcgttatc gaacgtcagt ccgtagatca
    11761 gccggtacag accggttata aagccgttga ctccatgatc ccaatcggtc gtggtcagcg
    11821 tgaattgatc atcggtgacc gtcagacagg taaaaccgca ctggctatcg atgccatcat
    11881 caaccagcgc gattccggta tcaaatgtat ctatgtcgct atcggccaga aagcgtccac
    11941 catttctaac gtggtacgta aactggaaga gcacggcgca ctggctaaca ccatcgttgt
    12001 ggtagcaacc gcgtctgaat ccgctgcact gcaatacctg gcaccgtatg ccggttgcgc
    12061 aatgggcgaa tacttccgtg accgcggtga agatgcgctg atcatttacg atgacctgtc
    12121 taaacaggct gttgcttacc gtcagatctc cctgctgctc cgtcgtccgc caggacgtga
    12181 agcattcccg ggcgacgttt tctacctcca ctctcgtctg ctggagcgtg ctgcacgtgt
    12241 taacgccgaa tacgttgaag ccttcaccaa aggtgaagtg aaagggaaaa ccggttctct
    12301 gaccgcactg ccgattatcg aaactcaggc gggtgacgtt tctgcgttcg ttccgaccaa
    12361 cgtaatctcc attaccgatg gtcagatctt cctggaaacc aacctgttca acgccggtat
    12421 tcgtcctgcg gttaacccgg gtatttccgt atcccgtgtt ggtggtgcag cacagaccaa
    12481 gatcatgaaa aaactgtccg gtggtatccg taccgctctg gcacagtatc gtgaactggc
    12541 agcgttctct cagtttgcat ccgaccttga cgatgcaaca cgtaagcagc ttgaccacgg
    12601 tcagaaagtg accgaactgc tgaaacagaa acagtatgcg ccgatgtccg ttgcgcagca
    12661 gtctctggtt ctgttcgcag cagaacgtgg ttacctggcg gatgttgaac tgtcgaaaat
    12721 tggcagcttc gaagccgctc tgctggctta cgtcgaccgt gatcacgctc cgttgatgca
    12781 agagatcaac cagaccggtg gctacaacga cgaaatcgaa ggcaagctga aaggcatcct
    12841 cgattccttc aaagcaaccc aatcctggta acgtctggag gcttgcctta gggcaggccg
    12901 caaggcattg aggagaagct catggccggc gcaaaagaga tacgtagtaa gatcgcaagc
    12961 gtccagaaca cgcaaaagat cactaaagcg atggagatgg tcgccgcttc caaaatgcgt
    13021 aaatcgcagg atcgcatggc ggccagccgt ccttatgcag aaaccatgcg caaagtgatt
    13081 ggtcaccttg cacacggtaa tctggaatat aagcaccctt acctggaaga ccgcgacgtt
    13141 aaacgcgtgg gctacctggt ggtgtcgacc gaccgtggtt tgtgcggtgg tttgaacatt
    13201 aacctgttca aaaaactgct ggcggaaatg aagacctgga ccgacaaagg cgttcaatgc
    13261 gacctcgcaa tgatcggctc gaaaggcgtg tcgttcttca actccgtggg cggcaatgtt
    13321 gttgcccagg tcaccggcat gggggataac ccttccctgt ccgaactgat cggtccggta
    13381 aaagtgatgt tgcaggccta cgacgaaggc cgtctggaca agctttacat tgtcagcaac
    13441 aaatttatta acaccatgtc tcaggttccg accatcagcc agctgctgcc gttaccggca
    13501 tcagatgatg atgatctgaa acataaatcc tgggattacc tgtacgaacc cgatccgaag
    13561 gcgttgctgg ataccctgct gcgtcgttat gtcgaatctc aggtttatca gggcgtggtt
    13621 gaaaacctgg ccagcgagca ggccgcccgt atggtggcga tgaaagccgc gaccgacaat
    13681 ggcggcagcc tgattaaaga gctgcagttg gtatacaaca aagctcgtca ggccagcatt
    13741 actcaggaac tcaccgagat cgtctcgggg gccgccgcgg tttaaacagg ttatttcgta
    13801 gaggatttaa gatggctact ggaaagattg tccaggtaat cggcgccgta gttgacgtcg
    13861 aattccctca ggatgccgta ccgcgcgtgt acgatgctct tgaggtgcaa aatggtaatg
    13921 agcgtctggt gctggaagtt cagcagcagc tcggcggcgg tatcgtgcgt accatcgcaa
    13981 tgggttcctc cgacggtctg cgtcgcggtc tggatgtaaa agacctcgaa cacccgatcg
    14041 aagtcccggt aggtaaagcg actctgggcc gtatcatgaa cgtactgggt gaaccggtcg
    14101 acatgaaagg cgagatcggt gaagaagagc gttgggcgat tcaccgcgca gcaccttcct
    14161 acgaagagct gtcaaactct caggaactgc tggaaaccgg tatcaaagtt atcgacctga
    14221 tgtgtccgtt cgctaagggc ggtaaagttg gtctgttcgg tggtgcgggt gtaggtaaaa
    14281 ccgtaaacat gatggagctc attcgtaaca tcgcgatcga gcactccggt tactctgtgt
    14341 ttgcgggcgt aggtgaacgt actcgtgagg gtaacgactt ctaccacgaa atgaccgact
    14401 ccaacgttat cgacaaagta tccctggtgt atggccagat gaacgagccg ccgggaaacc
    14461 gtctgcgcgt tgctctgacc ggtctgacca tggctgagaa attccgtgac gaaggtcgtg
    14521 acgttctgct gttcgttgac aacatctatc gttacaccct ggccggtacg gaagtatccg
    14581 cactgctggg ccgtatgcct tcagcggtag gttatcagcc gaccctggcg gaagagatgg
    14641 gcgttctgca ggaacgtatc acctccacca aaactggttc tatcacctcc gtacaggcgg
    14701 tatacgtacc tgcggatgac ttgactgacc cgtctccggc aaccaccttt gcgcaccttg
    14761 acgcaaccgt ggtactgagc cgtcagatcg cgtctctggg tatctacccg gccgttgacc
    14821 cgctggactc caccagccgt cagctggacc cgctggtggt tggtcaggaa cactacgaca
    14881 ccgcgcgtgg cgttcagtcc atcctgcaac gttatcagga actgaaagac atcatcgcca
    14941 tcctgggtat ggatgaactg tctgaagaag acaaactggt ggtagcgcgt gctcgtaaga
    15001 tccagcgctt cctgtcccag ccgttcttcg tggcagaagt attcaccggt tctccgggta
    15061 aatacgtctc cctgaaagac accatccgtg gctttaaagg catcatggaa ggcgaatacg
    15121 atcacctgcc ggagcaggcg ttctacatgg tcggttccat cgaagaagct gtggaaaaag
    15181 ccaaaaaact ttaacgcctt aatcggaggg tgatatggca atgacttacc acctggacgt
    15241 cgtcagcgca gagcaacaaa tgttctctgg tctggtcgag aaaatccagg taacgggtag
    15301 cgaaggtgaa ctggggatct accctggcca cgcaccgctg ctcaccgcca ttaagcctgg
    15361 tatgattcgc atcgtgaaac agcacggtca cgaagagttt atctatctgt ctggcggcat
    15421 tcttgaagtg cagcctggca acgtgaccgt tctggccgac accgcaattc gcggccagga
    15481 tctcgacgaa gcgcgagcca tggaagcgaa acgtaaggct gaagagcaca ttagcagctc
    15541 tcacggcgac gtagattacg ctcaggcgtc tgcggaactg gccaaagcga tcgcgcagct
    15601 gcgcgttatc gagttgacca aaaaagcgat gtaacaccgg cttgaaaagc acaaaagcca
    15661 gtctggaaac aggctggctt tttttacgcg ttgccccgtc ctgaagagcg ttcacataga
    15721 tcctgctgat ataaaacacc cctgttttcc cgtttattca ttgatcgaaa taagagcaaa
    15781 aacatccacc tgacgcttaa atgaaggtac tgccttaatt ttctgcagat aaaaggcgtg
    15841 acgatggtcg aaaatggcac tttcgtcagc ggggataatc cgttattgaa caatttatcc
    15901 tctgtccatt tcacgatgaa aaaaatgtag ttttttcaag gtgaagcggt ttaaattcgt
    15961 tctcaaatta cagtcaggac gcgtatgttg aataatgcta tgagcgtagt gatccttgcc
    16021 gcaggcaaag gcacgcgcat gtattccgat cttccgaaag tgctgcatac ccttgccggg
    16081 aaagcgatgg ttcagcatgt cattgatgct gcgaatgaat taggcgcagc gcacgttcac
    16141 ctggtgtacg gtcacggcgg cgatctgctt aaacaggcgc tgaaagacga caaccttaac
    16201 tgggtgcttc aggcagagca gctgggtacg ggtcatgcaa tgcagcaggc cgcacctttc
    16261 tttgccgatg atgaagacat tttaatgctc tacggcgacg tgccgctgat ctctgtcgaa
    16321 acactccagc gcctgcgtga tgctaaaccc cagggtggca ttggtctgct gacggtgaaa
    16381 ctggatgatc cgaccggtta tggacgtatc acccgtgaaa atggcaaagt taccggcatt
    16441 gttgagcaca aagatgccac cgacgagcag cgtcagattc aggagatcaa caccggcatt
    16501 ctgattgcca acggcgcaga tatgaaacgc tggctggcga agctgaccaa caataatgct
    16561 cagggcgaat actacatcac cgacattatt gcgctggcgt atcaggaagg gcgtgaaatc
    16621 gtcgccgttc atccgcaacg tttaagcgaa gtagaaggtg tgaataaccg cctgcaactc
    16681 tcccgtctgg agcgcgttta ccagtccgaa caggctgaaa aactgctgtt agcaggcgtt
    16741 atgctgcgcg atccggcgcg ttttgatctg cgcggtacgc ttactcacgg gcgcgatgtt
    16801 gaaattgata ctaacgttat catcgagggc aacgtgactc tcggtcatcg cgtgaaaatc
    16861 ggcaccggtt gcgtgattaa aaacagcgtg attggcgatg attgcgaaat cagtccgtat
    16921 accgttgtgg aagacgcgaa tctggcagcg gcctgtacta ttggcccgtt tgcccgtctg
    16981 cgtcctggtg ctgagttgct ggaaggtgca cacgttggta acttcgttga gatgaaaaaa
    17041 gcgcgtctgg gtaaaggcac gaaagccggg catctgactt acctgggcga tgcggaaatt
    17101 ggcgataacg ttaacatcgg cgcgggaacc attacctgca actacgatgg tgcgaataaa
    17161 tttaagacca ttatcggcga cgatgtgttt gtcggttccg acactcagct ggtggccccg
    17221 gtaacagtag gcaaaggcgc gaccattgct gcgggtacaa ctgtgacgcg taatgtcggc
    17281 gaaaatgcat tagctatcag ccgtgtgccg cagactcaga aagaaggctg gcgtcgtccg
    17341 gtaaagaaaa agtaattctg gccgggtaat ccggtcatct gggatgagga gataacataa
    17401 tctccctccc acaagcagta actataaaaa taaccccact ctctacaagg ctcggggcgc
    17461 ccgaaaaaac gggcatacag gttgaccgac aacgatataa atcggaatca aaaactatgt
    17521 gtggaattgt tggcgcgatc gcgcaacgtg atgtagcaga aatccttctt gaaggtttac
    17581 gtcgtctgga ataccgcgga tatgactctg ccggtctggc cgttgttgat gcggaaggtc
    17641 atatgactcg cctgcgtcgc ctcggtaaag tccagatgct ggctcaggca gcggaagaac
    17701 atcctctgca tggcggcacc ggtattgctc acactcgctg ggcgacccac ggtgaacctt
    17761 cagaagctaa cgcgcatccg catgtttctg aacacattgt ggtggtgcat aatggcatca
    17821 tcgaaaacca tgaaccgctg cgtgaagagt taaaagcgcg tggctatacc ttcgtttctg
    17881 aaaccgacac cgaagtgatt gcccatctgg tgaactggga gctgaaacaa ggcgggactc
    17941 tgcgtgaggc tgttctgcgt gctatcccgc agctgcgcgg tgcgtacggt acagtgatca
    18001 tggactcccg tcacccggat accctgctag cggcacgttc tggtagtccg ctggtgattg
    18061 gcctgggtat gggcgaaaac tttatcgctt ctgaccagct ggcgctgttg ccggtgaccc
    18121 gtcgctttat cttccttgaa gagggcgata ttgcggaaat cactcgccgt tcggtaaaca
    18181 tcttcgataa aactggcgcg gaagtaaaac gtcaggatat cgaatccaat ctgcaatatg
    18241 acgcgggcga taaaggtatt taccgtcact acatgcagaa agagatctac gaacagccga
    18301 acgcgataaa aaacaccctt accgggcgca tcagccacgg tcaggttgat ttaagcgagc
    18361 tgggaccgaa cgccgacgaa ctgctgtcga aggttgagca tattcagatc ctcgcctgtg
    18421 gtacttctta taactccggt atggtttccc gctactggtt tgaatcgctg gcaggtattc
    18481 cgtgcgacgt cgaaatcgcc tctgaattcc gctatcgtaa atctgccgtg cgtcgtaaca
    18541 gcctgatgat caccttgtca cagtctggcg aaaccgcgga taccctggcg ggcctgcgtc
    18601 tgtcgaaaga gctgggttac cttggttcgc tggcaatctg taacgttccg ggttcttctc
    18661 tggtgcgcga atccgatctg gcgctgatga ccaacgcggg tacggaaatc ggcgtggcgt
    18721 ccactaaagc attcaccact cagttaactg tgctgttgat gctggtggcg aagctgtctc
    18781 gcctgaaagg tctggatgcc tccattgaac atgacattgt gcatggtctg caggcgctgc
    18841 cgagccgtat tgagcagatg ctgtctcagg ataaacgcat tgaagctctg gcagaagatt
    18901 tctctgacaa acatcacgcg ctgttcctgg gccgtggcga tcagtaccca atcgcgctgg
    18961 aaggcgcatt gaagctgaaa gagatctctt acattcacgc tgaagcctac gctgcaggcg
    19021 agctgaaaca cggcccgctg gcgctgattg acgcggatat gccggttatc gtcgttgcac
    19081 cgaacaacga actgttggaa aaactgaaat ccaacattga agaagttcgc gcgcgtggcg
    19141 gtcaattgta tgtcttcgcc gatcaggatg cgggttttgt aagtagcgat aacatgcaca
    19201 tcatcgagat gccgcatgtg gaagaggtga ttgcaccgat cttctacacc gttccgctgc
    19261 agctgctggc ttaccatgtc gcgctgatca aaggcaccga cgttgaccag ccgcgtaacc
    19321 tggcaaaatc ggttacggtt gagtaatcac tatcatagat aaaaaagggc tgctgtgggc
    19381 ggacaataaa gtcttaaact gaacaaaata gatctaaact atgacaataa agtcttaaac
    19441 tagacagaat agttgtaaac tgaaatcagt ccagttatgc tgtgaaaaag catactggac
    19501 ttttgttatg gctaaagcaa actcttcatt ttctgaagtg caaattgccc gtcgtattaa
    19561 agaggggcgt ggccaagggc atggtaaaga ctatattcca tggctaacag tacaagaagt
    19621 tccttcttca ggtcgttccc accgtattta ttctcataag acgggacgag tccatcattt
    19681 gctatctgac ttagagcttg ctgtttttct cagtcttgag tgggagagca gcgtgctaga
    19741 tatacgcgag cagttcccct tattacctag tgataccagg cagattgcaa tagatagtgg
    19801 tattaagcat cctgttattc gtggtgtaga tcaggttatg tctactgatt ttttagtgga
    19861 ctgcaaagat ggtccttttg agcagtttgc tattcaagtc aaacctgcag cagccttaca
    19921 agacgagcgt accttagaaa aactagaact agagcgtcgc tattggcagc aaaagcaaat
    19981 tccttggttc atttttactg ataaagaaat aaatcccgta gtaaaagaaa atattgaatg
    20041 gctttattca gtgaaaacag aagaagtttc tgcggagctt ttagcacaac tatccccatt
    20101 ggcccatatc ctgcaagaaa aaggagatga aaacattatc aatgtctgta agcaggttga
    20161 tattgcttat gatttggagt taggcaaaac attgagtgag atacgagcct taaccgcaaa
    20221 tggttttatt aagttcaata tttataagtc tttcagggca aataagtgtg cagatctctg
    20281 tattagccaa gtagtgaata tggaggagtt gcgctatgtg gcaaattaat gaggttgtgc
    20341 tatttgataa tgatccgtat cgcattttgg ctatagagga tggccaagtt gtctggatgc
    20401 aaataagcgc tgataaagga gttccacaag ctagggctga gttgttgcta atgcagtatt
    20461 tagatgaagg ccgcttagtt agaactgatg acccttatgt acatcttgat ttagaagagc
    20521 cgtctgtaga ttctgtcagc ttccagaagc gcgaggagga ttatcgaaaa attcttccta
    20581 ttattaatag taaggatcgt ttcgacccta aagtcagaag cgaactcgtt gagcatgtgg
    20641 tccaagaaca taaggttact aaggctacag tttataagtt gttacgccgt tactggcagc
    20701 gtggtcaaac gcctaatgca ttaattcctg actacaaaaa cagcggtgca ccaggggaaa
    20761 gacgttcagc gacaggaaca gcaaagattg gccgagccag agaatatggt aagggtgaag
    20821 gaaccaaggt aacgcccgag attgaacgcc tttttaggtt gaccatagaa aagcacctgt
    20881 taaatcaaaa aggtacaaag accaccgttg cctatagacg atttgtggac ttgtttgctc
    20941 agtattttcc tcgcattccc caagaggatt acccaacact acgtcagttt cgttattttt
    21001 atgatcgaga ataccctaaa gctcagcgct taaagtctag agttaaagca ggggtatata
    21061 aaaaagacgt acgaccctta agtagtacag ccacttctca ggcgttaggc cctgggagtc
    21121 gttatgagat tgatgccacg attgctgata tttatttagt ggatcatcat gatcgccaaa
    21181 aaatcatagg aagaccaacg ctttacattg tgattgatgt gtttagtcgg atgatcacgg
    21241 gcttttatat cggctttgaa aatccgtctt atgtggtggc gatgcaggct tttgtaaatg
    21301 cttgctctga caaaacggcc atttgtgccc agcatgatat tgagattagt agctcagact
    21361 ggccgtgtgt aggtttgcca gatgtgttgc tagcggaccg tggcgaatta atgagtcatc
    21421 aggtcgaagc cttagtttct agttttaatg tgcgagtgga aagtgctcca cctagacgtg
    21481 gcgatgctaa aggcatagtg gaaagcactt ttagaacact acaagccgag tttaagtcct
    21541 ttgcacctgg cattgtagag ggcagtcgga tcaaaagcca tggtgaaaca gactataggt
    21601 tagatgcatc tctgtcggta tttgagttca cacaaattat tttgcgtacg atcttattca
    21661 gaaataacca tctggtgatg gataaatacg atcgagatgc tgattttcct acagatttac
    21721 cgtctattcc tgtccagcta tggcaatggg gtatgcagca tcgtacaggt agtttaaggg
    21781 ctgtggagca agagcagttg cgagtagcgt tactgcctcg ccgaaaggtc tctatttctt
    21841 catttggcgt taatttgtgg ggtttgtatt actcggggtc agagattctg cgtgagggtt
    21901 ggttgcagcg gagcactgat atagctagac ctcaacattt agaagcggct tatgacccag
    21961 tgctggttga tacgatttat ttgtttccgc aagttggcag ccgtgtattt tggcgctgta
    22021 atctgacgga acgtagtcgg cagtttaaag gtctctcatt ttgggaggtt tgggatatac
    22081 aagcacaaga aaaacacaat aaagccaatg cgaagcagga tgagttaact aaacgcaggg
    22141 agcttgaggc gtttattcag caaaccattc agaaagcgaa taagttaacg cccagtacta
    22201 ctgagcccaa atcaacacgc attaagcaga ttaaaactaa taaaaaagaa gccgtgacct
    22261 cggagcgtaa aaaacgtgcg gagcatttga agccaagctc ttcaggtgat gaggctaaag
    22321 ttattccttt caacgcagtg gaagcggatg atcaagaaga ttacagccta cccacatacg
    22381 tgcctgaatt atttcaggat ccaccagaaa aggatgagtc atgagtgcta cccggattca
    22441 agcagtttat cgtgatacgg gggtagaggc ttatcgtgat aatcctttta tcgaggcctt
    22501 accaccatta caagagtcag tgaatagtgc tgcatcactg aaatcctctt tacagcttac
    22561 ttcctctgac ttgcaaaagt cccgtgttat cagagctcat accatttgtc gtattccaga
    22621 tgactatttt cagccattag gtacgcattt gctactaagt gagcgtattt cggtcatgat
    22681 tcgaggtggc tacgtaggca gaaatcctaa aacaggagat ttacaaaagc atttacaaaa
    22741 tggttatgag cgtgttcaaa cgggagagtt ggagacattt cgctttgagg aggcacgatc
    22801 tacggcacaa agcttattgt taattggttg ttctggtagt gggaagacga cctctcttca
    22861 tcgtattcta gccacgtatc ctcaggtgat ttaccatcgt gaactcaatg tagagcaggt
    22921 ggtgtatttg aaaatagact gctcgcataa tggttcgcta aaagaaatct gcttgaattt
    22981 tttcagagcg ttggatcgag ccttgggctc gaactatgag cgtcgttatg gcttaaaacg
    23041 tcatggtata gaaaccatgt tggctttgat gtcgcaaata gccaatgcac atgctttagg
    23101 gttgttggtt attgatgaaa ttcagcattt aagccgctct cgttcgggtg gatctcaaga
    23161 gatgctgaac ttttttgtga cgatggtgaa tattattggc gtaccagtga tgttgattgg
    23221 tacccctaaa gcacgagaga tttttgaggc tgatttgcgg tctgcacgta gaggggcagg
    23281 gtttggagct atattctggg atcctataca acaaacgcaa cgtggaaagc ccaatcaaga
    23341 gtggatcgct tttacggata atctttggca attacagctt ttacaacgca aagatgcgct
    23401 gttatcggat gaggtccgtg atgtgtggta tgagctaagc caaggagtga tggacattgt
    23461 agtaaaactt tttgtactcg ctcagctccg tgcgctagct ttaggcaatg agcgtattac
    23521 cgctggttta ttgcggcaag tgtatcaaga tgagttaaag cctgtgcacc ccatgctaga
    23581 ggcattacgc tcgggtatcc cagaacgcat tgctcgttat tctgatctag tcgttcccga
    23641 gattgataaa cggttaatcc aacttcagct agatatcgca gcgatacaag aacaaacacc
    23701 agaagaaaaa gcccttcaag agttagatac cgaagatcag cgtcatttat atctgatgct
    23761 gaaagaggat tacgattcaa gcctgttaat tcccactatt aaaaaagcgt ttagccagaa
    23821 tccaacgatg acaagacaaa agttactgcc tcttgttttg cagtggttga tggaaggcga
    23881 aacggtagtg tcagaactag aaaagccctc caagagtaaa aaggtttcgg ctataaaggt
    23941 agtcaagccc agcgactggg atagcttgcc tgatacggat ttacgttata tctattcaca
    24001 acgccaacct gaaaaaacca tgcatgaacg gttaaaaggg aaaggggtaa tagtggatat
    24061 ggcgagctta tttaaacaag caggttagcc atgagaaact ttcctgttcc gtactcgaat
    24121 gagctgattt atagcactat tgcacgggca ggcgtttatc aagggattgt tagtcctaag
    24181 cagctgttgg atgaggtgta tggcaaccgc aaggtggtcg ctaccttagg tctgccctcg
    24241 catttaggtg tgatagcaag acatctacat caaacaggac gttacgctgt tcagcagctt
    24301 atttatgagc ataccttatt ccctttatat gctccgtttg taggcaagga gcgccgagac
    24361 gaagctattc ggttaatgga gtaccaagcg caaggtgcgg tgcatttaat gctaggagtc
    24421 gctgcttcta gagttaagag cgataaccgc tttagatact gccctgattg cgttgctctt
    24481 cagctaaata ggtatgggga agccttttgg caacgagatt ggtatttgcc cgctttgcca
    24541 tattgtccaa aacacggtgc tttagtcttc tttgatagag ctgtagatga tcactgacat
    24601 caattttggg ctttgggtca tactgagctg ctttcagact accccaaaga ctccctatct
    24661 caattaacag cactagctgc ttatatagcc cctctgttag atgctccacg agcgcaagag
    24721 ctttccccaa gccttgagca gtggacgctg ttttatcagc gcttagcgca ggatctaggg
    24781 ctaaccaaaa gcaagcacat tcgtcatgac ttggtggcgg agagagtgag gcagactttt
    24841 agtgatgagg cactagagaa actggattta aagttggcag agaacaagga cacgtgttgg
    24901 ctgaaaagta tattccgtaa gcatagaaaa gcctttagtt atttacagca tagtattgtg
    24961 tggcaagcct tattgccaaa actaacggtt atagaagcgc tacagcaggc aagtgctctt
    25021 actgagcact ctataacgac aagacctgtt agccagtctg tgcaacctaa ctctgaagat
    25081 ttatctgtta agcataaaga ctggcagcaa ctagtgcata aataccaagg aattaaggcg
    25141 gcaagacagt ctttagaggg tggggtgcta tacgcttggc tttaccgaca tgacagggat
    25201 tggctagttc actggaatca acagcatcaa caagagcgtc tggcacccgc ccctagagtt
    25261 gattggaacc aaagagatcg aattgctgta cgacaactat taagaatcat aaagcgtcta
    25321 gatagtagcc ttgatcaccc aagagcgaca tcgagctggc tgttaaagca aactcctaac
    25381 ggaacctctc ttgcaaaaaa tctacagaaa ctgcctttgg tagcgctttg cttaaagcgt
    25441 tactcagaga gtgtggaaga ttatcaaatt agacggatta gccaagcttt tattaagctt
    25501 aaacaggaag atgttgagct taggcgctgg cgattattaa gaagtgcaac gttatctaaa
    25561 gagcggataa ctgaggaagc acaaagattc ttggaaatgg tttatgggga agagtgagtg
    25621 gttaggctag ctacatttaa tgacaatgtg caggttgtac atattggtca tttattccgt
    25681 aactcgggtc ataaggagtg gcgtattttt gtttggttta atccaatgca agaacggaaa
    25741 tggactcgat ttactcattt gcctttatta agtcgagcta aggtggttaa cagtacaaca
    25801 aagcaaataa ataaggcgga tcgtgtgatt gagtttgaag catcggatct tcaacgagcc
    25861 aaaataatcg attttcctaa tctctcgtcc tttgcttccg tacgcaacaa ggatggagcg
    25921 cagagttcat ttatttacga agctgaaaca ccatatagca agactcgtta tcacatccca
    25981 cagttagagc tagctcggtc attattttta attaactcct atttctgtcg aagctgtttg
    26041 agcagtaccg ctttacagca agagttcgac gttcagtatg aggttgagcg agatcattta
    26101 gagataagga tcttacccag ttcatcgttt cctaaagggg cgttagagct gtcggccgta
    26161 gtgcagcttt tggtttggtt gttttcggat caagatgtta tggattcgta tgaaagtatt
    26221 tttaggcact atcaacaaaa tagagaaatt aagaacggcg ttgaaagctg gtgctttagc
    26281 tttgaccctc cgcccatgca gggttggaaa ttacatgtaa aaggacgttc ttctaacgag
    26341 gataaggatt atttagttga ggaaatagta ggtttagaaa tcaacgctat gcttcctagc
    26401 acaacagcta ttagccatgc ctcttttcag gaaaaggagg caggtgatgg tagtacgcag
    26461 cacatagcgg tttcaacaga gtcagttgtt gatgatgagc atctacagtt ggacgatgag
    26521 gaaacagcca atatagacac agacacacga gtcatagagg ctgagccgac atggataagt
    26581 tttagtagac ctagtcgaat tgaaaaatct cgcagggcaa gaaaaagtag ccaaactatt
    26641 ttagaaaaag aagaagcaac aacaagtgaa aatagtaatt tggttagtac tgatgagcca
    26701 cacttaggtg gtgtcctagc agcggcagat gtgggtggga agcaggatgc aaccaattac
    26761 aactctattt ttgctaatcg atttgctgct tttgatgagc tactttcaat tctaaaaact
    26821 aaatttgcat gtcgggtgct ttttgaagaa accttggttt tgccaaaagt tgggcgtagc
    26881 cgattacatc tgtgtaaaga tggctcacca agagtgatta aagccgttgg ggtgcaacgt
    26941 aatggcagtg aatttgtatt gctagaggtg gatgcatcgg atggggtgaa aatgctttct
    27001 accaaagtgt tgagtggcgt tgatagcgaa acatggcgga atgattttga aaagatacgg
    27061 cgtggagtgg tgaagagctc attgaattgg ccaaatagtt tgtttgatca attatatgga
    27121 caagacgggc atagaggggt gaatcatcca aaggggttgg gggagctgca agtatcgaga
    27181 gaggatatgg aagggtgggc tgagagagtg gttagagagc aatttacgca ttaaaggaat
    27241 gactgaaaga gcctgtaaac ccttttgtgt aagtgctttt gccggtcagt taaaggtggc
    27301 catttaaacg gtcaccaaat tcgatcataa aacggttcat ggccggcttc cagttgcgga
    27361 tcggcatcgt ccatttcttg gtcgccgcct ggatagccag gtacaccacc ttcatcgctg
    27421 attcgtccgt agggaacacc ttgagttcat attaatggaa ttttctacaa atagcctccg
    27481 tggttttgag gggggattac agacgatcca tagtagtaat ccaatgagtt cttgagcgcg
    27541 gcgacatgtt tggacgcctt ggcaaaaatt agagcctgct tgaagtgcag gcgagcacgt
    27601 gcttggacga ttgatccatt cgcggtcaaa aactcaatct tggatgacag cgtgtcagga
    27661 aatccaatat cgaagtcccg ccccgtgtca aagtagggca tttcatgatc aaaggacgaa
    27721 gcttcccagg ccacttccct aatattccag ccgctagaaa caagatttcg tacgacttca
    27781 tcaacttttt tgctttccaa cttgtcacaa agtgcatagg caagagcttt ggcggcagac
    27841 acgtgcgttt cctcttaaac ataggtcgaa cggatacccg aggcaaagcg gagcattagt
    27901 cccaaagcat ctccggtaga tccggaggct ccatactgaa ggcatggcat cttgggcatt
    27961 gatatttgaa acttctctct agatagcagg gatttcttgg ccttgcctca tgctgatagc
    28021 ttgtaaatat cacacgatcc ttagccccac aaagtgagca tactccgtag ctttcattta
    28081 tagaaatcgt cgttagattg tcgcagcatg tgcacgttcc gactatttgc tcaggaagca
    28141 tcgccatacc ttggcctagg taaagaagat cagactcgta gccacatcgg tggcaactga
    28201 gcttgtacgc aatgcccatc tggttgtctc acctgaatcg taacgtcaga gctattcatc
    28261 catccattcg gcatcaatgt atgccactaa gccgaccagc tgaccgtttg gactgaagta
    28321 tgttgcctgc accccacttg acatctcatc aataccaccg cactgcgcta ggcgccggtc
    28381 ccacttgccc acagaagcgt caggatcggc tgagttcccc aagacccttg tgagccatgc
    28441 ctcctttaac tcaacctctc gaatgattgc gtcggccagc tcctccgcgt tatctagttc
    28501 gacatagtct ggaattcgga cgtacgttct gaacacagct ggatacttac ttgggcgatt
    28561 gtcatacatg acatcaacaa tgtacccgtt tgtgtaaccg tctcttggag gttcgtatga
    28621 cactagtggt tcccctcagc ttgcgactag atgttgaggc ctaacatttt attagagagc
    28681 aggctagttg cttagataca tgatcttcag gccgttatct gtcagggcaa gcgaaaattg
    28741 gccatttatg acgaccaatg ccccgcagaa gctcccatct ttgccgccat agacgccgcg
    28801 cccccctttt ggggtgtaga acatcctttt gccagatgtg gaaaagaagt tcgttgtccc
    28861 attgttggca atgacgtagt agccggcgaa agtgcgagac ccatttgcgc tatatataag
    28921 cctacgattt ccgttgcgac tattgtcgta attggatgaa ctattatcgt agttgctctc
    28981 agagttgtcg taatttgatg gactattgtc gtaattgctt atggagttgt cgtagttgct
    29041 tggagaaatg tcgtagttgg atggggagta gtcataggga agacgagctt catccactaa
    29101 aacaattggc aggtcagcaa gtgcctgccc cgatgccatc gcaagtacga ggcttagaac
    29161 caccttcaac agatcgcgca tagtcttccc cagctctcta acgcttgagt taagccgcgc
    29221 cgcgaagcgg cgtcggcttg aacgaattgt tagacattat ttgccgacta ccttggtgat
    29281 ctcgcctttc acgtagtgaa caaattcttc caactgatct gcgcgcgagg ccaagcgatc
    29341 ttcttgtcca a
//
LOCUS       DAJTSI010000040        29043 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000040 DAJTSI010000000
VERSION     DAJTSI010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.040"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            109..1140
                     /gene="mdtN"
                     /locus_tag="OO327_001557"
     CDS             109..1140
                     /gene="mdtN"
                     /locus_tag="OO327_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR6018138.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1140..3182
                     /gene="mdtO"
                     /locus_tag="OO327_001558"
     CDS             1140..3182
                     /gene="mdtO"
                     /locus_tag="OO327_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR6018139.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            3179..4645
                     /gene="mdtP"
                     /locus_tag="OO327_001559"
     CDS             3179..4645
                     /gene="mdtP"
                     /locus_tag="OO327_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR6018140.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4843..5082
                     /locus_tag="OO327_001560"
     CDS             4843..5082
                     /locus_tag="OO327_001560"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018141.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            5092..5262
                     /locus_tag="OO327_001561"
     CDS             5092..5262
                     /locus_tag="OO327_001561"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR6018142.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            5311..6723
                     /gene="fdhF"
                     /locus_tag="OO327_001562"
     CDS             5311..6723
                     /gene="fdhF"
                     /locus_tag="OO327_001562"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCR6018143.1"
                     /translation="MSNAINEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIV
                     CDPRKIETARIADMHIALKNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKI
                     VEGYTPESVEDITGVSASEIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLA
                     MLTSNLGKPHAGVNPVRGQNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVES
                     LPAHTGYRISELPHRAAHGEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFI
                     TKTASAADVILPSTSWGEHEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMG
                     YPMHYNNTQEIWDELRHLCPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKF
                     DTPNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPG
                     YAQINTEDAKRLGIEDEALV"
     gene            6724..6990
                     /locus_tag="OO327_001563"
     CDS             6724..6990
                     /locus_tag="OO327_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HCR6018144.1"
                     /translation="MHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTESLSP
                     ITKTPEYKYCAVRVEPISDQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7084..7773
                     /locus_tag="OO327_001564"
     CDS             7084..7773
                     /locus_tag="OO327_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6018145.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="OO327_001565"
     CDS             complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="OO327_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR6018146.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="OO327_001566"
     CDS             complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="OO327_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR6018147.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="OO327_001567"
     CDS             complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="OO327_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR6018148.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10475..12193)
                     /locus_tag="OO327_001568"
     CDS             complement(10475..12193)
                     /locus_tag="OO327_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR6018149.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="OO327_001569"
     CDS             complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="OO327_001569"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR6018150.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="OO327_001570"
     CDS             complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="OO327_001570"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR6018151.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="OO327_001571"
     CDS             complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="OO327_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR6018152.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="OO327_001572"
     CDS             complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="OO327_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR6018153.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16274..18232
                     /gene="acs"
                     /locus_tag="OO327_001573"
                     /pseudo
     CDS             16274..18232
                     /gene="acs"
                     /locus_tag="OO327_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            18432..18746
                     /locus_tag="OO327_001574"
     CDS             18432..18746
                     /locus_tag="OO327_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCR6018154.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18743..20392
                     /gene="actP"
                     /locus_tag="OO327_001575"
     CDS             18743..20392
                     /gene="actP"
                     /locus_tag="OO327_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR6018155.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20570..21862
                     /locus_tag="OO327_001576"
     CDS             20570..21862
                     /locus_tag="OO327_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR6018156.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(22016..23665)
                     /locus_tag="OO327_001577"
     CDS             complement(22016..23665)
                     /locus_tag="OO327_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR6018157.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23816..25165)
                     /gene="ghxP"
                     /locus_tag="OO327_001578"
                     /gene_synonym="yjcD"
     CDS             complement(23816..25165)
                     /gene="ghxP"
                     /locus_tag="OO327_001578"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR6018158.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25871..26335)
                     /gene="soxR"
                     /locus_tag="OO327_001579"
     CDS             complement(25871..26335)
                     /gene="soxR"
                     /locus_tag="OO327_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR6018159.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26421..26744
                     /gene="soxS"
                     /locus_tag="OO327_001580"
     CDS             26421..26744
                     /gene="soxS"
                     /locus_tag="OO327_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR6018160.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26747..28324)
                     /gene="pdeC"
                     /locus_tag="OO327_001581"
                     /pseudo
     CDS             complement(26747..28324)
                     /gene="pdeC"
                     /locus_tag="OO327_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            28753..29034
                     /locus_tag="OO327_001582"
     CDS             28753..29034
                     /locus_tag="OO327_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCR6018161.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         7284 a         7776 c         7351 g         6632 t
ORIGIN      
        1 cgggcaaata tcaatctggc atttgtcgga attatttata ccgccctttt tgctctctac
       61 gccatgctgt tctggctggc atttttctaa tctatttgag acgcgctcat ggaaagtacg
      121 ccgaaaaaag cacctcgcag taaattccct gctctgttag tggttgcgtt ggcgctggtt
      181 gcccttgttt tcgttatctg gcgcgtagac agtgcgccat caactaatga cgcttacgcg
      241 tcagcagata ccattgatgt ggtgccggaa gtcagcggtc gcattgtaga actggcggtc
      301 accgacaacc aggcggtgaa acagggcgat ttgctgttcc gcatcgaccc gcgcccgtac
      361 gaagccaacc tggcgaaagc cgaagcctcc ctcgcggcgc tggataagca aattatgctc
      421 acccagcgta gcgttgacgc ccaacagttt ggtgccgact cggttaatgc cacggtagaa
      481 aaagcccgtg ccgccgcgaa acaggccaca gatacattac gccgcaccga gccattactg
      541 aaagagggtt ttgtctcagc agaagacgtt gaccgtgcaa gaacggcgca gcgcgccgca
      601 gaagcggatc ttaatgccgt attgttacag gcgcagtcag ccgccagcgc cgtcagcggc
      661 gtcgatgcgc tggtggcgca acgtgcggcg gtcgaagcgg atattgccct gaccaaacta
      721 catctggaaa tgacgaccgt tcgcgcgcca tttgatggcc ggatcatttc cctcaaaacc
      781 tccgtcgggc aatttgcttc tgccatgcgc cctattttta ccctcatcga cactcgtcac
      841 tggtacgtga tcgccaactt ccgcgaaacc gatctgaaaa atattcgctc aggtacaccc
      901 gcaacgattc gcctgatgag tgacagcggc aaaaccttcg agggtaaagt ggattcgatt
      961 ggctacggcg tgctaccgga tgacggcggc ctggtgctgg gcggcctgcc aaaagtgtct
     1021 cgttctatta actgggtccg cgttgcccag cgttttccgg tcaaaatcat ggtcgataaa
     1081 cctgacccgg aaatgttccg catcggcgct tcggcagtcg ctaatcttga gccgcaataa
     1141 tgagcgcgct caactccctg ccattaccgg tggtcaggct gctggcgttc tttcatgaag
     1201 agttaagcga gcggcgaccc ggccgcgtgc cgcagatcgt gcaactctgg gtaggctgcc
     1261 tgctggtgat tctgatctcg atgacttttg agatcccttt tgtggcgtta tcgctggcag
     1321 tgctgtttta cggtattcag tcgaacgcgt tttacaccaa atttgtggcg atcttgtttg
     1381 tggttgccac ggtgctggag atcggcagcc tgtttttgat ctacaaatgg tcatacggcg
     1441 aaccgttgat ccgattgatc atcgccgggc cgatcctgat gggctgcatg tttttgatgc
     1501 gcacccatcg gctgggactg gtctttttcg ctgtcgccat ttacgggcaa accttccccg
     1561 ccatgctcga ctatccggaa gtagtcgtgc gcttaacgct gtggtgtatc gttgttggcc
     1621 tctatccaac cttgctgatg acgttaatcg gcgtgctgtg gtttcccaat cgtgccatta
     1681 cgcaaatgca tcaagcgctt aatgatcggc ttgatgatgc cattagccac ctgacggaca
     1741 gcctcgcacc gctacccgaa acgcggattg aaagagaggc gctggcgctg caaaaactga
     1801 atgtcttttg tctcgcggac gatgccaact ggcgaactca aagcgcatgg tggcaaagct
     1861 gcgtggcaac ggtaacctac atttactcga cgctgaatcg ctacgatccc acctcttttg
     1921 ctgattctca ggcaattatt gaatttcggc aaaaattagc ctcagaaatc aacaagctgc
     1981 agcatgccgt tgctgaaggt cagtgctggc aaagcgactg gcggctcagt gaaagtgaag
     2041 cggtggcggc acgggaatgt aacctggaga atatctgcca gacgttgtta caactgggcc
     2101 agatgaaccc gaatacgccg ccaacgcccg ccgccaaacc gccatcaatg gtcgccgatg
     2161 cttttaccaa tccagactat atacgctacg cggtaaaaac attgctcgcc tgtttgatct
     2221 gttacacctt ttacagcggc gtggactggg aaggcattca cacctgtatg ctgacctgcg
     2281 tgatcgtcgc taatccaaat gtcggttcgt cgtaccagaa gatggtgctg cgttttggcg
     2341 gggccttttg cggcgcgatt ctggcgctgt tattcacgct actggtcatg ccctggctgg
     2401 acaatattgt cgaattgctg tttgtgctgg caccgatttt cctgttgggc gcatggattg
     2461 ccaccagctc tgaacgctct tcttatatcg gcacacagat ggtggtcacc ttcgcgctcg
     2521 ccacgctcga aaacgttttt ggcccagtgt acgacctggt ggaaattcgc gatcgcgccc
     2581 tgggtatcat cattggtacc gtggtgtccg cgatgattta cacctttgtc tggcctgaaa
     2641 gtgaagcgcg cacgctgccg caaaaactgg ctggcgcgct gggtatgtta agtaaagtaa
     2701 tgcggatccc acgccagcag gaagtcacgg ctctgcgcac ttatctgcaa attcgtatcg
     2761 gtctgcatgc ggcgtttaat gcctgtgaag agatgtgcca acgcgtggcg ctggagcgtc
     2821 aactggacag cgaagaacgc gccttactga ttgaacgttc gcaaacggtt attcgtcagg
     2881 gccgcgatat ccttcacgcc tgggatgcca cctggaattc ggcgcaggcg ctggataacg
     2941 cactacagcc ggacagagca ggtcagtttg ccgacgccct ggagaaatac gctgccggtc
     3001 tggcaaccgc actcagccgt tctcctcaaa taacgcttga agagacaccc acctcgcagg
     3061 ccatcctgcc caccttatta aaacaggagc aacacgtctg ccagcttttc gcccgcttgc
     3121 cagactggac agccccggca ttaacgcccg ccacggaaca ggcacaagga gccacgcaat
     3181 gatcaatcgt caactttcac gtctgctgtt gtgcagcatt ctcggcagca cgacgctgat
     3241 ttccggctgt gccctggtac gtaaggattc tgcgcctcat caacagctca aaccggaaca
     3301 aatcaaactg gccgatgata ttcatcttgc cagctccggc tggccgcagg cgcagtggtg
     3361 gaaacaactc aatgacccgc agttggatgc cctgatccaa cggacgctaa gtggttcaca
     3421 caccctcgcc gaagcgaaac tgcgggaaga aaaagcgcaa tcgcaggccg atttgttaga
     3481 tgccggttca cagttgcagg tggcagcgtt agggatgctt aaccgccaac gtgtctcggc
     3541 gaacggcttt ttaagccctt atgcgatgga tgcgcccgca ctggggatgg acgggccgta
     3601 ctatacggaa gccacagtag gtttgtttgc cggactggat cttgatttgt ggggtgtgca
     3661 tcgctcagcg gttgccgccg ccattggcgc gcataatgcc gcgctggcag aaaccgcagc
     3721 agtagagcta tcgctgacca cgggcgtagc gcagctttat tacagtatgc aggccagcta
     3781 tcagatgctc gatctgttag aacaaactcg cgatgtgatt gattacgcgg tgaaagcgca
     3841 ccaaagtaaa gtggcgcacg gtctggaagc acaagtgcct ttccacggcg cgcgggcaca
     3901 gattctggcg gtcgataaac aaattgctgc cgtcaaaggg caaatcaccg aaacgagaga
     3961 atctctgcgc gcattgattg gcgcgggagc cagcgatatg ccggagatca aacctgtggc
     4021 attaccacga gtccagaccg gcattccggc gacactctct tatgagttgc tcgccagacg
     4081 cccggatctg caagccatgc gctggtatgt tcaggcgtca ttagatcagg tggattccgc
     4141 gcgggcgttg ttctatccga gctttgatat caaagcgttt ttcggccttg atgccatcca
     4201 cctggatacc ttattcaaaa aaaccagtcg ccagttcaac ttcatcccgg gtctgaaatt
     4261 gccgctgttt gacggtggac ggttgaatgc caatctcgaa ggcacgcgcg ccgccagcaa
     4321 catgatgatt gaacgttaca accagtcagt actgaacgcg gtgcgtgacg ttgccgtcaa
     4381 cggcacgcgt ctgcaaacgc tcaacgacga gcgagaaatg caggctgaac acgtggaagc
     4441 aacgcgcttc acccagcgcg ctgccgaggc cgcctatcag cgcggcttaa ccagccgctt
     4501 acaggccact gaagcccggt tgccagtact tgccgaagag atgtcattac tgatgctgga
     4561 cagccgccgg gtgatccaaa gcattcagtt gatgaaatcg ctgggcggcg ggtatcaggc
     4621 ggctcccgtc gtcgagaaaa aataaaatgt ctgccgcgtg atggctgtca cgcggtattt
     4681 cgtttcgtca cgtcaaaact gacgacagcc tgtttttcgt cagagttttg aataaatagt
     4741 gcccgtaata tcagggaatg accccaaata aaatgtggca taaaagatgc atactgtagt
     4801 cgagagcgcg tatgcgtgat ttgattaact ggagcgagac cgatgaaaaa agtcgtcacg
     4861 gtttgcccct attgcgcatc aggttgcaaa atcaacctgg tcgtcgataa cggcaaaatc
     4921 gtccgggcgg aggcagcgca ggggaaaacc aaccagggta ccctgtgtct gaagggttat
     4981 tatggctggg atttcattaa cgatacccag atcctgaccc cgcgcctgaa aacccccatg
     5041 atccgtcgcc agcgtggcgg caaactcgaa cctgtttcct gagatgaggc actgaattac
     5101 gttgccgagc gcctgagcgc catcaaagag aagtacggtc cggatgccat ccagacgacc
     5161 ggctcctcgc gtggtacggg taacgaaacc aactatgtaa tgcaaaaatt tgcgcgcgcc
     5221 gttattggta ccaataacgt tgactgctgc gctcgtgtct gacacggccc atcggttgca
     5281 ggtctgcacc aatcggtcgg taatggcgca atgagcaatg ctattaacga aattgataat
     5341 accgatttag tgttcgtttt cgggtacaac ccggcggatt cccacccaat cgtggcgaat
     5401 cacgtaatta acgctaaacg taacggggcg aaaattatcg tctgcgatcc gcgcaaaatt
     5461 gaaaccgcgc gcattgctga catgcacatt gcactgaaaa acggctcgaa catcgcgctg
     5521 ttgaatgcga tgggccatgt cattattgaa gaaaatctgt acgacaaagc gttcgtcgct
     5581 tcccgtacag aaggctttga agagtatcgt aaaatcgttg aaggctacac gccggagtcg
     5641 gttgaagata tcaccggtgt cagcgccagt gagattcgtc aggcggcacg gatgtatgcc
     5701 caggcgaaaa gcgccgccat cctgtggggc atgggtgtaa cccagttcta ccagggcgtg
     5761 gaaaccgtgc gttctctgac cagcctcgcg atgctgacca gtaacctcgg taagccgcat
     5821 gcgggtgtaa acccggttcg tggtcagaac aacgtgcagg gtgcctgcga tatgggcgcg
     5881 ctgccggata cgtatccggg ataccagtac gtgaaagatc cggctaaccg cgaaaaattc
     5941 gccaaagcct ggggcgtgga aagcctgcct gctcataccg gctatcgcat cagcgagctg
     6001 ccgcaccgcg cagcgcatgg cgaagtgcgt gccgcgtaca ttatgggcga agatccgctg
     6061 caaactgatg cggagctgtc ggcagtacgt aaagcctttg aagatctgga actggtcatc
     6121 gttcaggaca tctttataac caaaaccgcg tcggcggcgg atgtcatttt gccgtcaacg
     6181 tcgtggggcg agcatgaagg cgtgtttact gcggctgacc gtggcttcca gcgtttcttc
     6241 aaagcagttg aaccgaaatg ggatctgaaa acggactggc aaatcatcag tgaaatcgcc
     6301 acccgtatgg gttatccgat gcactacaac aacacccagg agatctggga tgagttgcgt
     6361 catctgtgcc cggatttcta cggtgcgact tacgagaaaa tgggcgaact gggcttcatt
     6421 cagtggcctt gccgcgatac ttcagatgcc gatcagggga cttcttatct gtttaaagag
     6481 aagtttgata ccccgaacgg tctggcgcag ttcttcacct gcgactgggt agcgccaatc
     6541 gacaaactca ccgacgagta cccgatggta ctgtcaacgg tgcgtgaagt tggtcactac
     6601 tcttgccgtt cgatgaccgg taactgtgcg gcactggcgg cgctggctga tgaacctggc
     6661 tacgcacaaa tcaataccga agacgccaaa cgtctgggta ttgaagatga ggcattggtt
     6721 tgagtgcact cgcgtaaagg caaaattatc acccgtgcgc aggtcagcga tcgtccgaac
     6781 aaaggggcga tttacatgac ctaccagtgg tggattggtg cctgtaacga gctggttacc
     6841 gaaagcttaa gcccgattac gaaaacgccg gaatacaaat actgcgccgt gcgcgtcgag
     6901 ccgatctccg atcagcgcgc cgccgagcag tacgtgattg acgagtacaa caagttgaaa
     6961 actcgcctgc gcgaagcggc actggcgtaa tacagtcctt tctacagcct cctttcggag
     7021 gctgtttttt tatccattcg aactctttat actggttact ccctacccaa tcgtattatc
     7081 aaaatgaaaa aaattatcgc attgatgttg tttttgacat tctttgccca cgcgaacgac
     7141 tccgagcctg gcagccagta tttaaaggca gcagaggccg gggaccgacg tgcacaatat
     7201 tttcttgccg acagctggtt tagctccggc gatttgagca aagccgaata ttgggcacag
     7261 aaagccgccg acagcggtga tgctgatgcc tgcgcgctgt tggcgcagat caaaatcacc
     7321 aatccggtca gtctggacta tccacaagca aaagttcttg cagagaaagc ggcgcaagcg
     7381 ggcagtaaag aaggtgaagt aacgctggcg catattctgg taaatactca agcgggtaaa
     7441 ccggattatc caaaggcgat ttcgctgtta gaaaacgcct cggaagatct ggagaatgac
     7501 tccgccgtcg atgcccaaat gctgcttggt ttgatttacg ccaacggcgt gggcattaag
     7561 gccgacgatg acaaggcaac ctggtatttc aaacgcagct ctgcaatttc ccgaaccggt
     7621 tattccgagt actgggcggg aatgatgttc ttaaacggtg aggaaggttt tatcgagaag
     7681 aacaaacaaa aggcgctgca ctggttgaac ctgagctgta tggaagggtt tgataccggg
     7741 tgtgaagagt ttgaaaaatt aaccaacggt taaggtaggc ctgataagac gcggcaagcg
     7801 tcgcatcagg catccacaca ttgccggggg atatccccgg caatcttcaa ttattgatcc
     7861 gcagttttat caaatttgcc cagcacttca cgctcataag ccagcgcctt tttgcggtca
     7921 aatttgtgtt cccacttggc aatcaccagc accgccagcg cattacccac cacgttcagc
     7981 gcagtacgcg ccatgtcgag gatacggtca acaccagcaa taaacgccag accttccagc
     8041 ggaataccta cgctacccag cgttgccagc aacaccacaa acgacacgcc agccacgcca
     8101 gcaatccctt tcgaggtcac catcagcgtc agcaccagaa tgatttcctg ccagatggac
     8161 aggtcaatgc catacagctg agcgatgaaa atagcggcaa tactttgata cagcgtcgaa
     8221 ccatcaaggt taaaagagta accggtcggc accacgaagc tggtgatcga cgccggtgct
     8281 ccgtaggctt ccatcttctc aataattcgc ggcagtacgc tttcagagct ggcagtggag
     8341 tacgccagaa tcagctcatc tttcagaata cgaatcagga tccagacgct taacccgcac
     8401 agacgtgcca caattcccag cactaccagc gcgaagaaca gaatggcgaa atgcaccagc
     8461 agcaccagtt tcgccagtgg ccacagagac gagaaaccaa agttagccac cgtcaccgca
     8521 atcagcgcaa acacacccac cggtgcataa cgcatcacca tgtgagtcac tttaaacatg
     8581 gtttcagaga tggagcggaa cacggtcacc agcggttcac gatgcgtcgc gggcagggaa
     8641 gaaagcccca gaccaaacag caccgagaaa aagatgatcg gcagcatttc gcctttcgcc
     8701 atcgacgcca caatgttcgt cggcaccagc gacaaaatcg tgcccataat gccgtgggaa
     8761 ctgctttgta ccgcttccgt agtgctctga tatttcgaga tatcgacggt cgccagctgc
     8821 gacatatcca ccccggcacc gggctggaag acgttcgcca gagtgatccc caaaatgatg
     8881 gcgacggtgg tgatcacctc gaagtaaata atggttttcg cgccaatacg cccgagctgt
     8941 ttggcatcac caacacccgc gatacccacc accagcgtgg agatcacaat cggcacaaca
     9001 atcattttga tcagatggat gaagatatca cccgccggag agagcaaatt gacgaccagc
     9061 cagtcgcggc tgtcgctatg gtagtgcagg tagcttccca ggagaatgcc cagcaccata
     9121 gcaaacagaa tctgccaggc caggctgaat tttatatttt tcatcagaat aacttcctca
     9181 atggagcgcc gttccccgtt cagaaacgcg agaacgtgac atactgccag ggcatgaatt
     9241 gtgttgcgtt ggtttgtaga tttttttaac gcttatgatc agtatcattt gcgcggtatg
     9301 ttgcgcaacc ctttaacaac agagcttttc gctgcattaa tgcataataa cgcgagttta
     9361 tggcaggatt aatgaataac catttgttat ggaaagattt ttatttgttt aatctgcaat
     9421 aatactgatt ggtgattatt tttggcatat gttcattcga ttatttttta tgcagctcaa
     9481 tgacataatc ttcatcacca gatgattaat ccgatctccg ctgcaacagt tttgccatat
     9541 taatcgactc gaccagctgt gtccggttga tccggggtga gttgagatcc atcacttttt
     9601 gccatagttc gatggcttgc gcgtaattcg cctgcataaa cgcatcggaa gccagcagca
     9661 tcagagcggt gatttcgtta gagtccagcg cgagggcttt gtcgatcatt gcgcgagtct
     9721 gggcggtcat atgctggctg gcctggtaat aaagcaccgt cgccagcgcc gcatacagtt
     9781 cagcgttctc accacgtagt tgcaacgcct gacgatacgc cagcagcgaa ttgctgtaat
     9841 cgttttgcca cagatagtac tcgcccagta acgcccattg ttcgctgttt tgtggattag
     9901 cgcggatttt atcctgcaat gcctgaagct gcgcttccgg ggtttgctgg ctggcaaact
     9961 gatgtagcgg atcgcgctga cgctgatact ccgcacgtac cgcctgccat tttgggctta
    10021 acagataact gccgacacag aggaatacca tcaatatcgt gagtgttgtc agcattttca
    10081 ccggtatttg aggctgtttc atcgcttagc cctcactcgc cagaggatca gtgccatcag
    10141 caataacaac accactggca gcgcccacaa caccagcgtc tgacccgtta acggtgggtt
    10201 atagcgaaca aaatctccgt agcgttcggt catccagcca atgatttcga cttcgttctt
    10261 cccctccgac accatgctat aaacctgatg gcgcatactg accgccaccg gcgcattgga
    10321 ttcgagtaag ttttgattct ggcactgcgg acaacgtaac tggctggcaa tatttaacgc
    10381 ctgttgctgt tgttgcggat tggcgaattg ccaggtgtct acaacctgag cgcgcgcaaa
    10441 acaggtaaat aacagcagta gcgtgagaag ccctttattc atcgcgcttc ttcccccgcc
    10501 atccgcttaa caatgcgccc gcaatcatca acaatcctcc cccccagatc cagcgcacac
    10561 cgctttgtac atacaaacga aaagcgtaac gatccgctct ggttttttca cccatgaccg
    10621 cataccagtc atggatgccg ttccagcgaa ttgacggttc catcatttgc tgacggcgtg
    10681 cttcataaaa acgccgctcc ggcgttaatt caccaatgcg ttgctgatgg tcaaacagcg
    10741 ccactatcgc tttttcgctg gtgtaattgc ctctggcttg cagatcgagt cgctcaaaac
    10801 ggaaggtgta tcctgccagc gtcacctgct gacccggctg taaattcagg ctgatctcct
    10861 gacgactgac actggaaacc acgatccccg cggcaaataa cagcacgcca gcatgagcta
    10921 ccagcgccgg aagctgcacg cgtttgccag agacaaacgt cgccagcaca atcaccacca
    10981 gcatcaacag accaaacggt aacgtcgcgc ggttaaaata cggcgcgcca acggagaggc
    11041 gtccccagcc cagcaggccg taaatcatcg gataaagcgt tcccaccagc acgatcagta
    11101 gcactgcgca aaacagcaac agcgtagcga ggattaacat ttcccgcgat aaccccgaaa
    11161 aacgcaccgc cgggccaccg tcccgcgctc gccagccata cagagccaaa gacgcaaggc
    11221 taatcagtgc aaacaggctg aacaacggca cggcgcggac gttatccagt gcgaacgcat
    11281 gaaccgaaac cagaatgcca gaacggacaa ttaaggtgcc cagcagcgac agcatcagag
    11341 taactatcgc cagcaacagc gaccagtggc ggaaaatccc ccgctggcgt gtcagggaca
    11401 aactgtgcag cagcgcagtg gcagaaagcc agggtaataa agaggcgttt tccaccggat
    11461 cccagaacca ccagccgccc cagccgagtt cgcagtaggc ccaccaggaa ccgaggatga
    11521 tccccgccgt taatgcactc cagccaggta acgcccagcg ccagcaaatt cgggcgctgg
    11581 ccgcatcaaa ctcgccgcgc agtagactcg ccagcgccac gctcgccgct accatcaaac
    11641 cgccatagcc aaggtaaagc agcggtggat gaaagataag accgggatgt tgcagcatcg
    11701 gattgagatc gcggccttcg attgctggtg gaaatatccg cacgaaggga tcggaccaca
    11761 gcaccacaaa cagtagcagt gcggcgagca tgagagataa aacggctagc gtcagcggaa
    11821 atagcggatc gctttgctgc cgataatgcc aggcaaacag cgcgctccag gcggaaagca
    11881 gcagcaccca aagcagcagc gaaccttcat gaccgcccca caccgccgcc agtttgagtt
    11941 cccacgacag caggctgtag ctatgttggg cgacataaat gaccgagaaa tcgctgatga
    12001 gaaaacaata cgtcagtacg ccaaaggcga gcagcaggag cgcgaactgc gccagaatgc
    12061 cgatgcaagt gagtcgcatc atggcaggcc agcgcaaccg cactcccgca aaggccgtca
    12121 acggggtcaa cacgttgacc ccgagactta acaacaacgc cagaaaaccg acttcaggaa
    12181 ggaatacgtc cagaaaccac ctcttttctg gcttacgcca ccgtcaactg tccggcgtag
    12241 agaatgaaaa agcgcagcat caacacgccg accagactgg ccccacacgc cgccagcacg
    12301 gcaggaatgc cggaactgcg attgacccac ggtttgagca acattggcac aatcagcccc
    12361 agcccggcga caccaagcca gaaccaccac gtccagaaac cgccacctaa tgccgccacc
    12421 agcgcacgaa ctttaccgtc atcgcccagc gccagaccga caaaaaacgc caccagcagg
    12481 aagatttcac cccataccac ggggatttcc atccggtgaa caaagtgcgc ttccgtggaa
    12541 tgcgggttac tgcgttggcg tatcgccatg gcgatcagcg ccaccgccgc accggacgag
    12601 atgccggaga agaggagcag caccggcagg ctcgggttat tgaggaacgg atacgatttc
    12661 agcgccgaca gcagaaagcc ggtataagcc cccagcagca ccgccaacac cagcatcagc
    12721 gtttccagtc cgcgatgaac gggcgtcagc aggctcagaa ccttttgcac gatccccagc
    12781 ttcggcaacc agcgttgttg cagggcaagc aaatcatgtt caaagatttt cgccagccac
    12841 agcaccagca ccaccatgta gagctgaaac agcatcaccc ccatcgacat caccgaggta
    12901 aaactgtagt ggaacatcag cttccagaag gtccacggtc ttgtcaggtg gaagacgagg
    12961 atcaacagac cgaggatcac cgcgcccggc ccgacaatca gcgtggtgcg cagcaacgta
    13021 ctgtctgcac cgcccgcctg cgggtagaag cgacgtaaca gcacggccag cgtcaccaga
    13081 ccggcagaaa taccaatcaa aaacaggtag atggcaatcg gccagtccca caccagcgat
    13141 tcaaaatgaa atgcggaagt ctgcgtcatt ggctcacctc cccgtattta aacggtacgc
    13201 ggtacagctt cggtttggtt cccagcgcca gcttgtagcg gtaagtaggc ttctggcgca
    13261 gcagttgcga aatctcactg ttaggatcgt ccagattgcc aaacgtcagc gccttcgtcg
    13321 ggcaagcttc aacgcacgcg ggaagcttac cggcctgcaa attggtctta cgacagaaat
    13381 cgcatttgtc cgccgtcttc gtgaccggat ggataaagcg cacgcggtac ggacaggcgg
    13441 cgatgcagta ctgacaaccg acgcagagat caggattaac atcgacaatc ccgctagcgg
    13501 catcacgaaa agacgcaccg gtcgggcaga cgtcaacgca cggcgcatga tcgcagtgct
    13561 ggcaagactt acggaagaag cgatatttca catcaggaaa ttcgccctgc ggctcgctac
    13621 gaataatcgt caggcgcgag acgccttccg gcaccttgtt cacttcccga caagcatcca
    13681 tacaggcggt acagccgatg cataacgact cgtcatgcac catgccgtaa cgcaccccat
    13741 tgatattcaa cgttttcgcc accacgcgcc ctgcggtgcc gctgacggct gccagcacgc
    13801 cgacgccggt gagaaactgg cgacgagacc aggtcatggc tgctccttaa gcaacggaac
    13861 agacgccggg ttaaagttcg gattggtgcg ctgatcgctg tggcaatcga cgcaaatctt
    13921 aatccgtcct ttgtcactta acgtctgcat cgtatcttgc tgtggatgca gggaatggca
    13981 gctggcgcac gccactttgg ttacgtggac atcgtgcggc cagaacgctt tttgcaactg
    14041 ttccggcaga tgacaggaca tacagacgct gttctgctct ccgaccttat acatcggctc
    14101 gttaaagcgc atcacatctt tcaccccttc gcggtgttgc ggtgatgctt ggccgtgaca
    14161 gttggtgcag gtgaccggca atttgttatt cgggttgata acggaagcat gtttgccatg
    14221 cataccttcg gtatctggtt tatgacagtc cagacaggcg gcatccgggt tacgctgctg
    14281 ggtaacttcg tagcgatcac cagatgcctg caccgcaggg gtagcggtaa ttccgttcag
    14341 gctccacaac aggcccgacg ccagcacccc agcagttaat aacgaacgta atacgctcat
    14401 attcactcca ttgtgttttc cgccccttgc gaggcggaac ggggttattg gcttaacaga
    14461 ccgtttttac gtgcctgctc ttcccactgc gggatcaccg ttttgatgaa gtcctgcttc
    14521 tcggccttga tttgttccat gttcaggcca atggcctgct gggctttctc tttggtcgag
    14581 atatccggga tctggatttc atgggtgata cctttggtcg ccagcaggcg cgccagtttg
    14641 gtgcgcgcat ccgccgcttt atccatcgcc gtaccgagca tccgtagacc ttcttccggt
    14701 gcgtgcatat gaataccgtg ggaagcaatc gccagatccc aacgccactg ggcatgacgg
    14761 atatcgtcct gaatcggctt catttcggct tccgtcgcgc ctgcatccag cgccgctttc
    14821 gcttcgaagt gagcgtgaac cagttgatct tcaaccttga ttttcaggtc gttaatcgat
    14881 tgcttacgtt ccgcgaccac tttttgcaag gcagctttat cctgggtatg gcagttcgca
    14941 caagtctggg cgaagttatc aaacggatta ccaattttat ggtcggtgta gagtttgcct
    15001 tcggcgttct gcacttttgg catatggcag tcgatacagg tcacgttgtt tttaccgtga
    15061 atgcccgctg tccaggtttc atattccggg tgctgcgctt tcagcattgg cgttttcgac
    15121 agggagttag tccagtcaga gaaggcaatt ttgtcgtaat actgctccat attttcgact
    15181 ttcatgccgt catcccacgg gaatttaacc gctttgtttt tgccgtcgaa gtaatactcc
    15241 acatggcact gaccgcaaac catcgattgc tggtcgaaac gtccggcttt ctcaaaaggt
    15301 ttaccaatgg cttccatcgc gcgagccgca tacggacggg aaagggttaa ctccggtttg
    15361 cctttggcga actctggtga ggcggtgtta tggcagtcgg cacaacctaa gttgttgacg
    15421 atttccgggc cgccgcgcgc ccatttaccg tggaagtagc catcttcgcc gtctttctgg
    15481 atcagacgcg ccacatccgg gcttttacaa ctccagcacg ccatcggtag cggaccatct
    15541 tcagcgtttt tcggcgcacc ggtacgcagg gtttcacgca catcggtcac agcaaaggca
    15601 tgtccacgag gcttgttgta atcgcgcgag aagggatacc ccgcccacag gatcaccagc
    15661 cgtggatctt ccgccagggc gtcaacacgc tctgactgtt ccgaggtggc tttccaggag
    15721 agatattgat cgtgatgctg cggggcaaag gtttcattct tcgcttccac agttacaggt
    15781 tttgcgggag ctgccgtttg ttcagcgtga acagaagtga aaaagaaaaa aggaatcaat
    15841 aagctgaaga tacggcgtgc gtttattttt atccttgtca taggggcttc atccgaattg
    15901 cgccattgtt gcaatggcgg tttttattgt ttttcacgac agtaaccgca cctacactgt
    15961 catgacattg ctcaccccta tgtgtaacaa ataaccacac tgtgaatgtt gtctttaatc
    16021 aattgtaagt gcatgtgaaa taccacttta gagttagtca gtatcttcct ctttttcaac
    16081 agcatgcata actgcatgtt cctcaaagaa ttaatcaact tttgttggtg accttcaaaa
    16141 attaccctgc cgtttatttg caccattcta cttttgcgtg atctgtcgcc caaatactaa
    16201 acaaaactgc caatacccct acatttaacg cttatgccac atattattaa catcctacaa
    16261 ggagaacaaa agcatgagcc aaattcacaa acacaccatt cctgccaaca tcgcagaccg
    16321 ttgcctgata aaccctcagc agtacgaggc gatgtatcaa caatctatta acgtacctga
    16381 taccttctgg ggcgaacagg gaaaaattct cgactggatc aaaccgtacc agaaggtgaa
    16441 aaatacctcc tttgcccccg gtaatgtgtc cattaaatgg tacgaggacg gcacgctgaa
    16501 tctggcggca aactgccttg accgccatct gcaagaaaac ggcgatcgca ccgccatcat
    16561 ctgggaaggc gacgacgcca gccagagcaa acatatcagc tataaagagc tgcaccgcga
    16621 cgtctgccgc ttcgccaata ccctgctcga gctgggcatt aaaaaaggtg atgtggtggc
    16681 gatttatatg ccgatggtgc cggaagccgc ggttgcgatg ctggcctgcg cccgcattgg
    16741 cgcgatgcat tcggtgattt tcggcggctt ctcgccggaa gccgttgccg ggcgcattat
    16801 tgattccaac tcacgactgg tgatcacttc cgacgaaggc gtgcgcgccg ggcgtagtat
    16861 tccgctgaag aaaaacgttg atgacgcact aaaaaacccg aacgtcacca gcgtagagca
    16921 tgtggtggta ctgaagcgta ctggcggaaa catcgactgg caggaagggc gcgacctgtg
    16981 gtggcacgac ctggttgagc aagccagcga tcagcactag gcggaagaga tgaacgccga
    17041 agatccgctg tttattctct atacctccgg ttctaccgga aaaccaaaag gcgtactgca
    17101 cactaccggc ggttatctgg tgtacgcggc gctgaccttt aaatatgtct ttgattatca
    17161 tccgggtgat atctactggt gcaccgccga tgtgggctgg gttaccggac acagttattt
    17221 gctgtacggc ccgctggcct gcggcgcaac cacgctgatg tttgaaggcg taccgaactg
    17281 gccgacgcct gcccgtatgg cgcaggtggt ggacaagcat caggtcaata ttctctatac
    17341 cgcacccacg gcgatccgcg cgctgatggc ggaaggcgat aaagcgattg aaggcaccga
    17401 ccgttcgtcg ctacgcattc tcggttccgt gggcgagcca attaacccgg aagcgtggga
    17461 gtggtactgg aaaaaaatcg gcaacgagaa atgtccggtg gtcgatacct ggtggcagac
    17521 cgaaaccggc ggtttcatga tcaccccgct gcctggcgct accgagctga aagccggttc
    17581 ggcaacacgt ccgttcttcg gcgtgcaacc ggcgctggtc gataacgaag gtaacccgct
    17641 ggaaggggct accgaaggta gcctggtgat caccgactcc tggcctggtc aggcgcgtac
    17701 gctgtttggc gatcacgaac gttttgagca gacctatttt tccaccttca aaaatatgta
    17761 tttcagcggc gacggcgcgc gtcgcgatga agatggctat tactggatca ccgggcgtgt
    17821 ggacgacgtg ctgaacgtct ccggtcaccg tctggggacg gcagagatag agtcggcgct
    17881 ggtggcgcat ccgaagattg ccgaagccgc cgtagtaggt attccgcaca atattaaagg
    17941 tcaggcgatc tacgcctatg tcacgcttaa tcacggggag gaaccgtcac cagaactgta
    18001 cgcagaagtc cgcaactggg tgcgtaaaga gattggcccg ctggcgacgc cagacgtgct
    18061 gcactggacc gactccctgc ctaaaacccg ctccggcaaa attatgcgcc gtattctgcg
    18121 caaaattgcg gcgggcgata ccagcaacct gggcgatacc tcgacgcttg ccgatcctgg
    18181 cgtggtcgag aagctgcttg aagagaagca ggctatcgcg atgccatcgt aacccacaat
    18241 tgccggatgc gaatcggtaa cggtttgtag gcctgataag acgcgacagc gtcgcatcag
    18301 gcattgattg ccggatgcgg cgtaaacgcc ttatccggcc tacattcggc aagggttacc
    18361 cgagcgttaa ccttctccca taagggagcg tgaattaaaa caatccctac attacctctg
    18421 gagaatctgt gatgaatggt acgatttatc agcggataga agacaatgcg catttcaggg
    18481 agttagtcga aaaacggcaa cggtttgcca ccatcctgtc gattattatg ctggcagttt
    18541 atatcggctt tattttactg atcgccttcg cgcccggctg gctgggcacg ccgctgaatc
    18601 cgaacaccag cgtcacacgc ggtattccaa ttggtgttgg agtgattgtg atctcctttg
    18661 ttctcaccgg tatctacatc tggcgggcga acggcgaatt cgaccgtctt aataatgaag
    18721 tcctgcatga ggtacaagca tcatgaaaag agttctgacg gcgcttgccg ccacactccc
    18781 tttcgcagct aacgccgcgg atgctattag cggggccgta gagcgccagc caacgaactg
    18841 gcaggcgatt attatgttcc tgattttcgt cgtgtttacg ctcggcatta cctactgggc
    18901 atcaaaacgc gtacgttctc gtagcgacta ctacaccgca ggcggcaata tcactggctt
    18961 ccagaacggg ctggcgattg ccggggacta tatgtccgcc gcctcattct tggggatctc
    19021 cgcgctggtg tttacctccg gctatgacgg gctgatctac tcgttgggct tcctggtggg
    19081 ctggccaatc attctgtttc tgattgccga acgtctgcgt aacctgggac gctacacctc
    19141 tgccgatgtg gcctcttatc gtctgaaaca agggccgatt cgtattcttt cggcctgtgg
    19201 ttctctggta gtggtggcgc tttaccttat cgctcagatg gtaggcgcag gtaaactgat
    19261 cgagctgctg tttggcctta actatcacat tgcggtggtg ctggtcggcg tgctgatgat
    19321 gatgtacgtc ctgttcggcg gcatgctggc gaccacctgg gtacaaatta tcaaagctgt
    19381 gctgttgctg ttcggtgcca gctttatggc ctttatggtg atgaaacacg tcggctttag
    19441 cttcaacaat ctgttcagcg aagcgatggc ggtacacccg aaaggtgtcg acatcatgaa
    19501 accgggcgga ctggtgaaag atccgatctc cgcgctctct ctggggctgg gactgatgtt
    19561 tggtacggcg ggcttgccgc acattctgat gcgcttcttt acagtcagcg atgcccgcga
    19621 agcacgtaag agcgtgttct acgccaccgg atttatgggc tacttctata ttctgacctt
    19681 tattatcggc ttcggcgcga tcatgcttgt tggtgcgaat ccggaatata aagacgcggc
    19741 gggccatctg attggtggta acaacatggc ggccgttcac ctggcgaatg cagtgggcgg
    19801 caacctattc ctcggtttta tttcagcggt tgctttcgcc actatcctcg cggtggttgc
    19861 ggatctgacg ctggcgggcg catcggcggt ttcgcatgac ttgtacgcta acgtcttcaa
    19921 aaaaggcgcg accgaacgtg aagagctgcg ggtatcaaaa atcaccgtac tgatcctcgg
    19981 cgtgattgcg attatcctcg gcgtgctgtt tgagaatcag aacatcgcct ttatggtagg
    20041 ccttgcgttt gccatcgcgg cgagctgtaa cttcccgatc attctgcttt ccatgtactg
    20101 gtcgaaactg actacccgtg gcgcgatgct gggcggctgg ctggggctga ttaccgcagt
    20161 ggtgctgatg atcctcggcc cgactatttg ggtacagatc ctcggtcacg aaaaagccat
    20221 cttcccgtat gaatacccgg cgctgttctc tatcagcgtg gcattcctcg gcatctggtt
    20281 cttctcggca accgataact cagcggaagg ggcgcgcgag cgtgaactgt tccgcgcgca
    20341 gtttatccgc tcccagaccg gctttggcgt tgagcaaggc cgcgcacatt aatctctccc
    20401 cttccccggt cgcctgaccg gggaatacgc ttcctctcca acatgcatca ccttttccca
    20461 taatattaaa caaataaact cattaaaaaa tgagcgattt ttgacagtcg tagaaaatga
    20521 taatgcagag aatatgcctt ttctttcttg ttaattataa ggatatttta tgcgctataa
    20581 tggtttaaat aatatgtttt tccctctttg ccagattaac gataaccact ctatcacaag
    20641 ttcatcacat acaaagaaaa caaaatcaga taattacagc aaacatcata aaaacacgtt
    20701 aattgacaat aaagccctct ctcttttcaa aatagatgat catgaaaaag tgataggctt
    20761 gattcagaaa atgaaaagaa tttatgatag tttaccatca ggaaaaatca cgaaagaaac
    20821 ggacaggaaa atacataaat attttataga tatagcgtta tatgcaaata ataaatgtga
    20881 cgatagaatt acgagaagag tttaccttag taaagaaaag gatgtatcca ttaaggtggt
    20941 atattttata aataatgtcg ccgtccataa taatactatc gaaattccac agatagtaaa
    21001 tggtggttac gatttttcac accttagcct gaagggtatc gtgattaaag atgaagattt
    21061 atccaattcg aattttgcag gttgcagact acaaaacgct attttccagg actgtaatat
    21121 gtataaaacg aatttttatt acgccataat ggaaaaaata ctttttgata attgtattct
    21181 cgatgactca aatttcgctc agataaaaat ggccgacgga actctaaatg catgctccgc
    21241 tatgcatgtt caattctaca atgcagcaat gaatagagcc aatattaaaa ataccttcct
    21301 tgactattca aatttttata tggcgtacat ggctgaggta aatctttata aagtaatagc
    21361 gccatatgtt aatttattta aagccgacct tagtttctct aaactcgatt taattaactt
    21421 tgaacatgct gatctgtctc gcgtcaatct gaacaaagca atcctccaga atataaactt
    21481 aattgatagc aaactctttt gtacgtggct gacaaataca ttcctcgaaa tggttatatg
    21541 taccggctct aatatggcta atgttaattt taataatgcc aatttaagca actgccattt
    21601 caactgttct attttaacaa aagcctgtat gtttaatacc cgtctctatc gggttaattt
    21661 cgatgaggct agcgtccagg gaatgggcat ttccattctc cgtggggagg aaaatatccc
    21721 cattgatagt gataccctgg taacactaca gaaattcttt gaagaagatt gtacctctca
    21781 tactggcatg tcacaaactg aggataatat taatgcagtc gctatgaaga ttactgcaga
    21841 tattatgcaa cacgcagatt gaggttaagt cgaagaacag ccaatgaatt gactttacaa
    21901 gagagataac ttatttgttt tagaatcatg aatgttcgcc cgagctaacc tgttaaaggt
    21961 gcaaaagatt gcaccttttt cagattacct cgggcctggt tacgtcgctt tcggcttact
    22021 gattttcctc aattagcaac gcttcaagca aatcgagatc gtgcagtaat ttttgcagcg
    22081 tttcgttgct gatctcccgc gtggcgcgca ggtggtacag ttcagcacgt tcagaacgca
    22141 atgccgccag acggaagcga cgctcaaggt tctcttcctg cacggaactt tcaacgtcgt
    22201 tacgtccatc ggcgcgacga cgcaggttac caatgacgcg agaactgacc tccgtaagca
    22261 actggttatc gatattctct tcggtatccg ccgccagacg ctcctccatt ttctggatcg
    22321 ccacaatcgc cacttctgcc gttgccgctc gcgcaatacg ttcttctttc aattgttgcg
    22381 aatggtccgc gacttcaatg tgttgtagca gaataggcaa catcaccacg ccgacaaaca
    22441 gcgagaagag aatgacgcca gccgccagga acaccagctc atagcgcgcc gggaagacgt
    22501 taccatccgg caagagcagc gggattgaga gcacaccggc cagagtgatc gccccacgca
    22561 ccccagcgaa agacgcgatc aggatttctc gtgtggtcca cgaaccaaac tccatcggct
    22621 tcttcttcag gaagcggttg ctgaactttt tcatcgtcca cagccagccg aaacggacca
    22681 gcatcagcgc cgcatatatc agaataatat cggtaaacag catccagatt tcgacgttag
    22741 ggtcgatttc tgccgccatc agcgacgtct ccagaatacc cggcagttgc agacctaaca
    22801 gcaggaacac catgccgtta aagacaaatt ccagcatcgc ccaggtgctg tttgcgcgca
    22861 ggcgcattgc cagtggcgca cggcgcatca caccggagcg ggtgatggtc atcccggcgg
    22921 caaccgcagc gaggatgccc gaaacgccaa tatgttcggc aatcagataa gaagcgaatg
    22981 gcagcaagaa cagcagaacg atctgcgtcg cgggttcatc accgccccag cggctgagga
    23041 atcgcagcga gcgaccatac agccagctca ccacaaaacc ggcaagaata ccgccaatgg
    23101 ctactttcat aaattcgacc gtcgcaccgc cgacagtgaa gatcatcgtc cccatcgcca
    23161 ctgccacggc aaacttcaac gacaccaggc cggaagcgtc attcatcaac gcttcgccct
    23221 gcaaaatgcc catgattttt ttcgggatgc gcccttcgcc aacaatcccg gagagcgcca
    23281 cagcatcggt cggagaaagt accgccgcca gcgcaaaggc ggggatcagc ggaatgcccg
    23341 gcaccaccca gtaaataagg aagccaatgc cgaccacggt gaccaccacc agcgccagcg
    23401 cgaggccgaa aatctctcga ccatgttcga gaaattcacg ggtcggcgtt ttccagccat
    23461 cagcgaacag caacggcggg ataaacaaga ctaaaaagag ttcaggatca aactccacat
    23521 gcaaaccaaa cgtcggccac gccagtagcg caccgatggc gatttgcata agcggaagcg
    23581 ggatctgaaa gggcatgaca cgagtgacca ccccggacag cgagaccacg agggtcatta
    23641 tcagtatggt gaagaagatt tccatgcgtt ccctgtttgc gatgtttttt atgatctgag
    23701 aaagacgtta tgcctgaatt ctcatttttt cagaatatcg caaaagctca cacaatgtgc
    23761 ggagaaaata aaaacgggca gccattgcca cccgttttca gaatccgatg aaagattaga
    23821 tagcccagcc acccgcatag aaggtcacca gcgccacggc gataacgacc gtaccgatgt
    23881 tcaacttgcg ccattcaccg gaaaccagac gaccaatcac cagagtcgcg aagccgatca
    23941 tgatgcctgt tacgatgtta caggtcagca cgatgaatac cgccgtaacc agacccgcca
    24001 tcgcatcaac aaaatcagca aagtcgattt tcgccacgtt gctcagcatc agcaggccaa
    24061 cgtacatcag cgccggagcc gttgcatacc ccggaacgag gtaagaaagc ggagagagga
    24121 acagaatcag caggaacagc acgccaacgg tgatagccgt caacccggtt ttaccgcccg
    24181 ccgccgtacc cgccgcagac tcgatgtata ccgctgccgg agctgcaccc accaggccag
    24241 agaaaacgct gctcatggag tcagtggtca gtgctttccc gccgtcgatg atctgcccat
    24301 ctttatccag caggttcgcc tggccggcga cagcgcggat agttccggtg gcatcaaata
    24361 ctgccgtcat caccagcgcc agaacgcttg gcaggactac aggattcagc gcgcccataa
    24421 tgtccaggct gccaatcagt gaattgccgt tttcatcgct caatgaaggc atggcgaaaa
    24481 cgccggagaa atggacgtta ggatcgaaga tcaaaccgac aattgagata ccgataatgg
    24541 tcagcagaat gccaccaggg actttcagtt tttccaagcc gatgatcacc gccagacctg
    24601 ccagtgacat aatcaccggg aaggtcgcga aatcacccag cgcaacgggc agaccatcaa
    24661 gcgggttttt aatcaccaga ccgacaccat tagcggcaat gagcagcagg aacagaccga
    24721 taccgatccc cgtgccgtgc gccacaccgt gcggcaagtt gcgcaaaatc cagctacgga
    24781 tacccgtggc agaaattacc gtaaacagca cacccatcag gaacacggca cccagcgcga
    24841 caggtacgct aatatgttgc cccagcacca ggctgaatgc ggtaaacgcc gtcagggaga
    24901 tggcgcaacc aatcgccaac ggcagattag cccacagacc catcacgata gaaccgagtc
    24961 cggcaaccag acaggttgca acgaaaactg ccgcaggcgg gaagcctgct ttacccaaca
    25021 tacctggaac gacgatgacc gagtagacca tcgccagaaa cgttgttaac ccggcaacca
    25081 cttcctgacg gacagtgctt ccacgttgtg aaattttaaa ccaggcgtcg agtgaaccgc
    25141 cggtacgcgc tgatggcgta gacataagaa aacatcccct gagagtttaa ttttcgtcaa
    25201 gatgcgtggc ggacaatccc ctgccagcga aacgttatct tcctgctcca ggtttgcggc
    25261 aatttggtgc gtcgcaaaaa agcaaacgtt tagctccgcg catacaaaac gggtggtaaa
    25321 aaaaagcaaa cgattatccg gcgatcatag cggctttttc aaccgaagtt tgctgctttt
    25381 ttctctttta ttgtcttgac gatgaaaaac caggcaattg atgcgcaaac gttatcgtcg
    25441 atgtgcaaga ttgcagcgtt atcgagccgt ataccgttct ggattgcgat aaaaaatcgt
    25501 ctgagatgac aaaaatattg cggctaaaaa gtatactttt acactggcga agaaacgtcg
    25561 gtgacataca attaaagcat caacaccaac cggaacctcc accacgtgct cgaatgaggt
    25621 gtgttgacgt cgggggaaac cctcctgtgt tccagcggga tagagagaaa gacaaagacc
    25681 ggaaaacaaa ctaaagcgcc cttgtggcgc tttagttttg tgtttgcgga taattccaaa
    25741 agagctcgat cagtcccctc gccccttcgg ggagagggtt agggtgaggg gaacaggcag
    25801 caccagtgca aatatttacc ctcaccccgg ccccatagtt aacgatgagt cagcttgtcc
    25861 ttgtggcgct ttagttttgt tcatcttcca gcaagcgtgc gccggtacct tcttctccta
    25921 agcggtcgcc cgggttacgc aacgggcaat cactgcgcga aaggcagcca caaccaatac
    25981 atccgtccag ttcgtcacgc agcgccacta aggtatgaat gcgccgatcc aactcttctc
    26041 gccattggga cgaaagctgt ttccactctt tcgcacttaa cgtatgccct tctggcaaca
    26101 cgccaaacgc ttcaccaatg gtcgccagcg gaatgccaat acgctgagcg attttgataa
    26161 ttgcgacata tcgcaacaca tcacgtttat atcgccgctg attgccgctg ttacggatac
    26221 tagtaatcaa ccctttgctt tcatagaaat gcagcgccga taccgccaca ccgctgcgtt
    26281 tcgccacttc gccaggggtt agcagcgctt taatgcgggg taatttcttt tccataaatc
    26341 gctttacctc aagttaactt gaggaattat actccccaac agatgaatta acgaactgaa
    26401 cactgaaaag aggcagattt atgtcccatc agaaaattat tcaggatctt atcgcatgga
    26461 ttgacgtgca tattgaccag ccgcttaaca ttgatgtcgt cgcaaaaaaa tcaggctatt
    26521 caaagtggta cttgcaacgg atgttccgca cggtgacgca tcagacgctt ggcgattaca
    26581 ttcgccaacg ccgcctgtta ctggccgccg ttgagttgcg caccaccgag cgtccgattt
    26641 ttgatatcgc aatggacctg ggttatgtct cgcagcagac cttctcccgc gttttccgtc
    26701 ggcagtttga tcgcactccc agcgattatc gccaccgcct gtagttttat tgcccgcgcg
    26761 ttaactcccg cgcgggtaat tgctccatcc attgcataaa cacctgcggc ggcatcgcct
    26821 tcgcaaaaaa ccatccctgg caatagcgca cgccgcgttt gcgcagccag ttaacctgct
    26881 cctcagtttc gacgccttca gcgatcgttt ttaaccccag gctgtgcgcc agctcgatga
    26941 tgtgttccgc aatcaaatga ctggttttgt gggtggtcag cgtttcaaca aacgatttat
    27001 cgattttcaa aatatcgaca ttcaatgatt taaggttatg caagttagag tagccaatac
    27061 caaaatcatc aattgccact tcgtaaccag cctggcggaa agccagaatt aatcggcgtc
    27121 attttttcga catcaagaaa tgcatgctca gtcacttcaa atttaatttg ctgcggacgc
    27181 accgcgtatt gctctgtttt ctgattgatt cgcgctatca accgtgacgt atgaaaatcg
    27241 gaggccgaca ggttaataga aacatagcga tcggcatgtg ttgccaggta cgcgcccaga
    27301 tcgcggaaga cattatcaat aacataatca gttatctgtt ctatcatccc ctccttttct
    27361 gccagcggaa taaactctgc cggattcatt atttgcccct gctcaccagg ccaacgtaac
    27421 aacgcttcag cgccgataca tttttctgtt ttgatatcga ttattggctg gtaataaaga
    27481 caaagttgat gtttttcgag ggcgcgttgc aatttacgtt tgggagataa atagttttgt
    27541 cgaatacgta gccagagtaa tagcagaaca agactcccca aaataccggc gggcaacgca
    27601 aaaataagat gattataaaa atgggttata agacgttgat atgaagtcgc aacaatggct
    27661 gcaattgggc gttttgtcga ataaattgtc gcatataaat agccatttct ttgtacggtt
    27721 aaatccttca aatgaatcag cggagaaaac gttgctgccg aggcctcttt gcttaacgag
    27781 aaaaaggttt tcatcaccgt atcatacaca ccccattgca atgtcgggtc atcagacatc
    27841 acttcactcc agaagagagg gttgataacc gccacataat ttccccgctg catataggtc
    27901 attttatagc cagagaaaaa aggcgtatcg cggtaataat agatagaaac gttaggttca
    27961 cgcttataat cggccggtgc aatcgtatag ccgtttacag gcgctatcag cgatgagcat
    28021 aaaaaatggt tatcacgaat tcattaatat aaagatagcc acgaataata ttcaacattc
    28081 gcttttgatg ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta
    28141 aatctgcctg gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga
    28201 gctgattatt cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg
    28261 gaaagagaac taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac
    28321 tcattgctat caatttcctg cctgtttata cccggccatt gcaaggtgaa accctggcta
    28381 ccgacgtctt tagataaatc ttacggtata aatagcactt ttgcctgatt tgaaattgat
    28441 acctttttaa tcgtcacctg gatgaaaata attcattgat attcaaaatt aattttctac
    28501 aggaatacag aggcggcggg aagatatgcc cttaatgcca ctgaaaaacc acataatttg
    28561 tacagcaata aaagtcacgg cctaatatgg ctaacaaaca atcaattgtg atatagttca
    28621 caaaattaat gaaacaaaca gagtgtttca tttttgtgtt ctgcaagccc cgcgatagcc
    28681 agagtgtacc acgcctcccg tgaacaacgc cgcgctgtcc agggcatcgg ctcttttcag
    28741 aggaattgtt tgatggctac cctcaccact ggcgtggttc ttcttcgctg gcaacttctt
    28801 agtgccgtaa tgatgtttct ggccagcaca ctcaacatcc gttttcgtcg gtctgattat
    28861 gtcgggcttg cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg
    28921 gggttgcttg gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg
    28981 atgatagaag agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta
    29041 gaa
//
LOCUS       DAJTSI010000041        28897 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000041 DAJTSI010000000
VERSION     DAJTSI010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28897
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.041"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(51..1400)
                     /gene="lysC"
                     /locus_tag="OO327_001583"
     CDS             complement(51..1400)
                     /gene="lysC"
                     /locus_tag="OO327_001583"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR6018162.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            1925..3574
                     /gene="pgi"
                     /locus_tag="OO327_001584"
     CDS             1925..3574
                     /gene="pgi"
                     /locus_tag="OO327_001584"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR6018163.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            4037..4279
                     /locus_tag="OO327_001585"
     CDS             4037..4279
                     /locus_tag="OO327_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018164.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            4393..5031
                     /locus_tag="OO327_001586"
     CDS             4393..5031
                     /locus_tag="OO327_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR6018165.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            5028..5765
                     /locus_tag="OO327_001587"
     CDS             5028..5765
                     /locus_tag="OO327_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR6018166.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            5765..7861
                     /locus_tag="OO327_001588"
     CDS             5765..7861
                     /locus_tag="OO327_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR6018167.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(7908..8186)
                     /locus_tag="OO327_001589"
     CDS             complement(7908..8186)
                     /locus_tag="OO327_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018168.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            8187..8330
                     /locus_tag="OO327_001590"
     CDS             8187..8330
                     /locus_tag="OO327_001590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018169.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            8456..8866
                     /gene="psiE"
                     /locus_tag="OO327_001591"
     CDS             8456..8866
                     /gene="psiE"
                     /locus_tag="OO327_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR6018170.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(8910..10385)
                     /gene="xylE"
                     /locus_tag="OO327_001592"
     CDS             complement(8910..10385)
                     /gene="xylE"
                     /locus_tag="OO327_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCR6018171.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFLMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(10756..11646)
                     /gene="malG"
                     /locus_tag="OO327_001593"
     CDS             complement(10756..11646)
                     /gene="malG"
                     /locus_tag="OO327_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR6018172.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(11661..13205)
                     /gene="malF"
                     /locus_tag="OO327_001594"
     CDS             complement(11661..13205)
                     /gene="malF"
                     /locus_tag="OO327_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCR6018173.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(13359..14549)
                     /gene="malE"
                     /locus_tag="OO327_001595"
     CDS             complement(13359..14549)
                     /gene="malE"
                     /locus_tag="OO327_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCR6018174.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            14914..16029
                     /gene="malK"
                     /locus_tag="OO327_001596"
     CDS             14914..16029
                     /gene="malK"
                     /locus_tag="OO327_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCR6018175.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            16101..17441
                     /gene="lamB"
                     /locus_tag="OO327_001597"
     CDS             16101..17441
                     /gene="lamB"
                     /locus_tag="OO327_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCR6018176.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            17593..18513
                     /gene="malM"
                     /locus_tag="OO327_001598"
     CDS             17593..18513
                     /gene="malM"
                     /locus_tag="OO327_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCR6018177.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            18742..20322
                     /locus_tag="OO327_001599"
                     /pseudo
     CDS             18742..20322
                     /locus_tag="OO327_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            20545..21042
                     /gene="ubiC"
                     /locus_tag="OO327_001600"
     CDS             20545..21042
                     /gene="ubiC"
                     /locus_tag="OO327_001600"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR6018178.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            21055..21927
                     /gene="ubiA"
                     /locus_tag="OO327_001601"
     CDS             21055..21927
                     /gene="ubiA"
                     /locus_tag="OO327_001601"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR6018179.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(22082..24505)
                     /gene="plsB"
                     /locus_tag="OO327_001602"
     CDS             complement(22082..24505)
                     /gene="plsB"
                     /locus_tag="OO327_001602"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR6018180.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            24676..25044
                     /gene="dgkA"
                     /locus_tag="OO327_001603"
     CDS             24676..25044
                     /gene="dgkA"
                     /locus_tag="OO327_001603"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR6018181.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            25154..25762
                     /gene="lexA"
                     /locus_tag="OO327_001604"
     CDS             25154..25762
                     /gene="lexA"
                     /locus_tag="OO327_001604"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR6018182.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            25835..27160
                     /gene="dinF"
                     /locus_tag="OO327_001605"
     CDS             25835..27160
                     /gene="dinF"
                     /locus_tag="OO327_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR6018183.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            27276..27485
                     /locus_tag="OO327_001606"
     CDS             27276..27485
                     /locus_tag="OO327_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR6018184.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(27527..28042)
                     /gene="zur"
                     /locus_tag="OO327_001607"
     CDS             complement(27527..28042)
                     /gene="zur"
                     /locus_tag="OO327_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR6018185.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            28360..28897
                     /locus_tag="OO327_001608"
                     /pseudo
     CDS             28360..28897
                     /locus_tag="OO327_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313057.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
BASE COUNT         7221 a         7113 c         7451 g         7112 t
ORIGIN      
        1 gacaagaaaa tcaatacggc ccgaaatata gcttccaggc catacagtat ttactcaaac
       61 aaattactat gcagtttttg caccacctgc tcggcatctt cgccgggcac caggaagcac
      121 aggttatggc tggatgcgcc gtaacaaatc atgcgaatgt tgaacggttc cagtacgccg
      181 aatacctctt tgccaacgcc gcaggctttt gacaggtcat tgccaatcaa cgcgaccagc
      241 gccagacctt cttccacctc cacccggcac agtgcggaaa gctccatcag cagagattgc
      301 gtcagcaacg tatcgccagt ggaggttgaa ccggtggtat caagggttaa tgccacgctc
      361 acttctgacg tggtgattaa gtctaccgaa atattatgcc gcgcgaggat gccgaaaact
      421 tccgcgagga agccgcgaga atgcagcata ttcaggctgt gcaaagtgag cagagtctga
      481 ttgcgacgaa gcgccagcgc gcggaacagc ggcggatttt cagttttatt gcacaccagc
      541 gtaccacctg cgcgtgggtc tttgctggag ccgacaaaga ccgggatatc gctgcgtact
      601 gcgggtagca acgttgccgg atgcagtact tttgcaccaa aagttgccat ctctgccgct
      661 tcggcaaacg cgatttcatc aatgcgtttt gctgcggaaa ctacgcgtgg atcggtggtg
      721 tagatgcccg ggacgtcggt ccagatatca acacgagatg cgtgtaaagc ctccgccagc
      781 aaggctgccg tataatcgct gcctccacgg ccaagcgtcg ttgtacgacc tttattttcg
      841 ctaccgataa atccctgggt gatcactaag ccttcattga ggcgtgggag cagctgcagc
      901 gcggccagtt ccgccagcgc ggctacatct ggctctgcac gaccaaatcg gtcgttggta
      961 cgcatcactt tacgtacatc aaaccactgt gcctgaacat cgcgttcgcg caggatttcg
     1021 acaaacagca gggtcgacat cagctcgccg tggctgacca gctcatctgt cagcgccgga
     1081 gacgttgcca gcgccgccgc ttctgccaga acagtaatgt tctccagcag acgttcaatc
     1141 tcttcacgga taacgttcgg gtaacgcaga cgttccagaa tggcaaactg gatgttgcga
     1201 atagcgtcga gtttttcgaa tcgctcgcca ggttccagcc cttccgctaa agcgaccagc
     1261 agattagtga taccagcaga agccgagagg actactaaac gcacgttggc atcagaaagc
     1321 acaatatcag cgctgcggtt catggcgtca aaatcagcta cgctggtacc gccaaatttg
     1381 gagacaacaa tttcagacat aactacctcg tgtcagggga tccattttca gccttggcac
     1441 aagggaagag cggaagacgg gtgggcgcag agcgatactt cgctactatt ttcacccaga
     1501 agtgctccac cacttgcgaa acgcccgact gcgaacgctt ctggtgacaa cccaggggat
     1561 tcagcccctg tagccgatga tgaacgtggc cagccgttca atcacctcgg cgatgcaccc
     1621 cctcaggtgt tatcacagga ctggctcctc caacaccgtt acttgggcaa cgcgcctctt
     1681 ctggcctgcg ctagcgcagg tagtacattt ataaataaag ggtgagcggg gcggttgtca
     1741 acgacggggt catgcggatt tttcatccac tcctggcggt cagtagttca gctaataaat
     1801 gcttcactgc gctaagggtt tacactcaac attacactaa cggcactaaa accatcacat
     1861 ttttctgtga ctggcgctac aatcttccaa agtcacaatt ctcaaaatca gaagagtatt
     1921 gctaatgaaa aacatcaatc caacgcagac cgctgcctgg caggcactac agaaacactt
     1981 cgatgaaatg aaagacgtta cgatcgccga tctttttgct aaagacggcg atcgtttttc
     2041 taagttctcc gcaaccttcg acgatcagat gctggtggat tactccaaaa accgcatcac
     2101 tgaagagacg ctggcgaaat tacaggatct ggcgaaagag tgcgatctgg cgggcgcgat
     2161 taagtcgatg ttctctggcg agaagatcaa ccgcactgaa aaccgcgccg tgctgcacgt
     2221 agcgctgcgt aatcgtagca ataccccgat tctggttgat ggcaaagatg ttatgccaga
     2281 agtcaacgcg gtgctggaga agatgaaaac cttctcggaa gcgattatct ccggtgagtg
     2341 gaagggttat accggcaaag caatcactga cgtagtgaac atcgggatcg gcggttctga
     2401 cctcggcccg tacatggtga ccgaagcact gcgtccgtac aaaaaccacc tgaacatgca
     2461 ctttgtttct aacgtcgatg ggactcacat ctcggaagtg ctgaaaaaag taaacccgga
     2521 aaccacgctg ttcctggtag catctaaaac cttcaccact caggaaacca tgaccaacgc
     2581 ccatagcgcg cgtgactggt tcctgaaagc ggcaggtgat gaaaaacacg ttgcgaaaca
     2641 ttttgcggcg ctctcaacca atgccaaagc cgttggcgag tttggtattg atactgccaa
     2701 catgttcgag ttctgggact gggtcggcgg ccgttactct ttgtggtcag cgattggcct
     2761 gtcgattgtt ctctccatcg gctttgataa cttcgttgaa ctgctttctg gcgcacacgc
     2821 aatggacaag catttctcca ccacgcctgc cgagaaaaac ctgcctgtcc tgctggcgct
     2881 gattggcatc tggtacaaca atttctttgg tgcggaaact gaagcgattc tgccgtatga
     2941 ccagtatatg caccgtttcg cggcgtactt ccagcagggc aatatggagt ccaacggtaa
     3001 gtatgttgac cgtaacggta acgttgtgga ttaccagact ggcccgatta tctggggtga
     3061 accaggcact aacggtcagc acgcgttcta ccagctgatc caccagggaa ccaaaatggt
     3121 accgtgcgat ttcatcgccc cggctatcac ccataacccg ctctctgatc accaccagaa
     3181 actgctgtct aacttcttcg cccagaccga agcgctggcg tttggtaaat cccgcgaagt
     3241 ggttgagcag gaatatcgcg atcagggtaa agatccggca acgcttgact acgtggtgcc
     3301 gttcaaagtg ttcgaaggta accgcccgac caactccatc ctgctgcgcg aaatcactcc
     3361 gttcagcctg ggtgcgttga ttgcgctgta tgagcacaaa atctttactc agggcgtgat
     3421 cctgaacatc ttcaccttcg accagtgggg cgtggagctg ggtaaacaac tggcaaaccg
     3481 tattctgcca gagctgaaag atgataaaga aatcagcagc cacgatagct cgaccaatgg
     3541 tctgattaac cgctataaag cgtggcgcgg ttaatcatca tgcgtgcctg atgccgatat
     3601 gtaggccgga taaggcgttt acgccgcatc cggcaaccgg agcctgatgc caatatcgac
     3661 attaatgcat agtatgagga gttaaataac cttaaaaaca atgccgacaa taagtcggca
     3721 ttgttttatc agataaatcc ccttgtctgt gatttaacgg aaatcatacc gtgaggttaa
     3781 tcctaaaata gatttttaat cgttgtttat ttcggagaat acgcagatta attacttttg
     3841 ttttttattt taagcttatg atttttattg ttatttaaat ataagttgaa acttatattt
     3901 gatattcatt ccaattatcc taaaactcca tcgctaattc cccgcgccgt aattcgcatg
     3961 ctttagttgt gtatactcga tcccgcccga aatgtttttg ggtaaatctc cattcattca
     4021 atgaagggaa attgttatga aaaaagttct gtatggcatt tttgccatat ctgcgcttgc
     4081 ggcgacttct gcgtgggctg cacctgtaca ggtgggcgaa gcggcagagt cggcagcaac
     4141 gtcggtttcg gcggggagtt cctccgcgac cagcgtcagc accgtaagct cggcggtggg
     4201 tgtcgcgctc gcggcaaccg gtggcggtga tggttctaat accgggacca ccacaaccac
     4261 gaccaccagt acccagtaat aaagtatgta tccccaaaat aatttgagtc attgcatctg
     4321 tggcttgaag tatgaaggga tttaccataa ccacactccg gtgtggttat tctgcccctc
     4381 tggagaagag tcgtgaagcg acctgcactc attcttatct gcctgctatt acaggcctgt
     4441 tcagccacga ctaaagagct gggcaattca ctgtgggaca gtctgttcgg cacgccaggc
     4501 gtacagctga cggacgatga tattcaaaat atgccctacg ccagccagta catgcagctt
     4561 aatggcgggc cgcaattgtt tgtggtgctg gccttcgctg aagacggaca acaaaaatgg
     4621 gtcactcagg atcaagccac tcttgtcaca caacatggtc gtctggtgaa gactttgctt
     4681 ggcggcgaca acctgattga agtgaataac cttgccgccg acccgctgat taaacccgca
     4741 caaattgttg atggtgcaac atggacgcgc acgatgggct ggaccgagta ccagcaggta
     4801 cgctacgcca ccgcacgctc agtcttcaaa tgggatggca ccgataccgt caaggtcggc
     4861 agcgatgaaa ccctggttcg cgtgctggac gaagaagtct ccaccgacca ggcgcgctgg
     4921 cataaccgct actggatcga cagcgaaggg caaattcgcc agtcggaaca gtatctcggc
     4981 gcggattatt tcccggtgaa aaccactctc atcaaggcgg caaaacaatg attaaacaaa
     5041 ctattgtcgc gttgcttttg agtgtgggag cgtcatcggt cttcgcggca ggaaccgtca
     5101 aggtgttcag caatggcagc agtgaggcca aaacgctaac gggcgcagag catttaatcg
     5161 atctggtagg gcaacctcga ctggcaaaca gctggtggcc tggtgcggtg attagcgaag
     5221 agctggcaac ggcggcagca ttgcgtcagc agcaggcgtt gctgacacgg ctggcagaac
     5281 agggcgcaga ttccagcacc gacgatgccg ccgccattaa cgccttacgc cagcaaatcc
     5341 aggcattgaa agtgacaggc aggcaaaaaa tcaatcttga tcccgatatc gtccgcgttg
     5401 ccgaacgcgg taacccgccg ttgcagggca actacacgct gtgggtcgga ccaccgccgt
     5461 ccacggtcac gttgttcggg cttatcagcc atcctggcaa tcagccattc actccaggtc
     5521 gcgacgtggc gagctatctc tctgaccaga gcctgctcag cggtgcggat cgcagctacg
     5581 cgtgggtcgt ttacccggac ggacgcacgc aaaaagcgcc ggtggcttac tggaacaagc
     5641 gtcacgtaga gccgatgccc ggcagcatta tttatgttgg cctcgcagat tccgtctgga
     5701 gtgagacgcc tgatgccctt aacgccgaca ttcttcagac tctgacgcag cggatacccc
     5761 aataatgaaa aaaagacatc tgcttagctt actggcgctg ggcattagca cagcttgcta
     5821 cggcgaaaca tatcctgcgc ccattggtcc gtcgcagtcg gatttcggtg gcgtaggatt
     5881 attacaaact cccaccgcgc gtatggcacg ggaaggggag ttgagtctga actatcgcga
     5941 taacgatcag taccgttatt actcagcttc agtgcaactc ttcccgtggc tggaaacaac
     6001 gctgcgctac accgacgtgc gcacccggca gtacagcagc gtcgaagcgt tctctggcga
     6061 tcaaacgtat aaagataaag ccttcgatct caaactgcgt ttgtgggaag agagttactg
     6121 gctgccgcaa gtggcggttg gtgcgcggga tatcggcggt acggggctgt ttgatgcgga
     6181 atatcttgtt gccagcaaag cctgggggcc gttcgatttt acgctcggcc tgggctgggg
     6241 atatctgggc accagcggta atgtaaaaaa tccgctctgt tcagccagtg ataaatattg
     6301 ctatcgcgat aacagctaca aacaggcggg atctatcgac ggtagccaga tgttccacgg
     6361 tcctgcctca ctgtttggcg gcgtggaata ccagacgccc tggcaaccgc tgcgtctgaa
     6421 actggagtat gaaggtaata attatcagca ggattttgcc gggaagctgg agcaaaaaag
     6481 taagtttaac gtcggggcga tttatcgcgt taccgattgg gccgacgtta accttagcta
     6541 tgaacgtggc aacaccttta tgtttggcgt cacgctgcgc accaacttta acgatctgcg
     6601 cccgtcctac atcgataacg cccgcccgca atatcaaccg cagccgcagg atgccatttt
     6661 gcagcattcg gtggtggcga atcagttaac gttgctgaaa tacaacgccg gacttgccga
     6721 tccgcagatc caggcgaaag gcgatacgct gtatgttacc ggcgagcagg tgaaatatcg
     6781 tgattcgcgc gaagggatca tccgtgctaa tcggatcgtg atgaacgatc tgccggatgg
     6841 gatcaaaacg atccgcatta cggaaaatcg ccttaacatg ccgcaagtga cgacggaaac
     6901 cgatgtcgcc agcctgaaaa atcatctcgc cggagagccg ttgggccacg aaacgacgct
     6961 ggcgcacaaa cgcgtcgagc cagtggttcc gaagtccacc gagcagggct ggtatatcga
     7021 caaatcacgc tttgatttcc atatcgatcc ggtgctgaac cagtcggtcg gtggcccgga
     7081 aaacttttac atgtatcagc tgggcgtgat gggaacggca gatttgtggc tgacggacca
     7141 tctactgacc accggcagcc tgtttgcaaa tcttgccaac aactacgaca agtttaacta
     7201 caccaatcca ccgcaggact cgcacttacc gcgcgttcgt acccatgtgc gcgagtatgt
     7261 gcagaacgat gtctatgtga ataacctgca agccaactac ttccagcatc tgggcaacgg
     7321 cttctacggt caggtctatg gaggttatct cgaaaccatg tttggcggtg cgggggccga
     7381 agtgttgtat cgtccgctgg acagtaactg ggcgtttggt ctggatgcca actacgttaa
     7441 acagcgcgac tggcgtagtg caaaagatat gatgaaattc accgactaca gcgtgaaaac
     7501 cggacatctg accgcctact ggacgccatc tttcgcccag gacgtgttgg tgaaagccag
     7561 cgtcgggcag tatctggcag gggataaagg cggcacgctg gagatcgcta aacgctttga
     7621 cagcggcgtg gtggtgggtg gctatgccac gatcaccaac gtttcgaaag aggagtacgg
     7681 cgaaggggac ttcaccaaag gcgtgtatgt ctcggtaccg ttggatctct tctcgtctgg
     7741 cccgacacgc agccgtgcgg cgattggctg gacgccgctg acgcgtgacg gtggtcagca
     7801 acttgggcgt aagttccagt tgtatgacat gaccagcgac cgtagcgtaa atttccgcta
     7861 agtcatggga aaggtgccag ttttcgcact cactggcacc ctcattctta atgacctgtt
     7921 gagccatcag gaacgcagtt cacccccggt ttacgatctg gtgtgtaaag gcgaaaacat
     7981 tctgtcttcc tgtccgtcga tatcggttgc aggtctggtt gcctgagcga ctgggccatc
     8041 attgggctgg agacattgct ttgcggtagg tcggctttat cagcagtacc gagcggacca
     8101 gcataagcag gaagaacaga gactgataac atcaaagcag caaaataagg cttcattttt
     8161 accaccttta tcaggttacg tttcatttgt tccagaggaa cattgtcgat ttttcgcgca
     8221 ttgctggtgg ctgggaatca cctgaatggg tgatttttga attaccggct ttggtgcggt
     8281 ttgtcttgcc ggatgcgccg ccaggcgcgg cttatccggc ctacgggtag gtatatccgg
     8341 ctttggtgga ggcgcgctcc aaatccaggt tgaacaaaac atacacaaaa aatatagatc
     8401 tccgtcacat ttttgcgtta tacaggaagc tcgccactgt gaaggaggta ctgctatgac
     8461 gtcactctct cgtccgcgcg tggagtttat ctccaccatt ttgcagaccg tactcaatct
     8521 tggcctgctg tgcctcggcc tgattttggt tgtcttcctc ggcaaagaaa cggtgcatct
     8581 ggctgatgtg ctgttcgcgc cagaacaaac cagcaaatat gagctggtag aaggactggt
     8641 ggtttacttt ctctatttcg aatttatcgc gctgattgtg aagtactttc agtccggttt
     8701 tcacttcccg ctacgttact ttgtctatat tgggatcacc gccattgtgc gcttgatcat
     8761 cgtcgatcat aaatcgccac tggatgtgtt gatctactcg gctgcgatcc tgttactggt
     8821 gatcaccctg tggctgtgta attcgaaacg gctgaagcgg gagtaaaaag acagcacgcc
     8881 gaaatggcgc ggcgtgctgg acaggaagat tacagcgtag cagtttgttg tgttttcttc
     8941 gtttccggtt cccagagcgc ttccagctcc tcaagggttt tacctttggt ttccgggaca
     9001 aatttccaca taaacagtgc tgccagaacg cccatacaac cgtaaatcca gtaggagaaa
     9061 ccgttgtgga aatgggccac cagccaggag tttttgtcca tcatcaggaa ggtccaggag
     9121 acgaagtagt tcgccagcca ctgggccgcc accgcgattg ccagcgcttt accacgaata
     9181 gcattcggga agatttccga cagcagtacc cagcataccg gaccccagga catggcaaag
     9241 gcggcaacat agaacagcat cgacagtagc gccacaatac ccgatgcctg agtgtaaaac
     9301 gcggtaccga ggctaaacat accgattgcc attccgagtg cgccgataat ttgcagtggc
     9361 ttacgaccaa atttatccac agtcataatt gccagtacgg taaaggtgag gttgataact
     9421 ccgacaataa tggtctgcaa cagcgcgata tccgtgctgg cccccagcgt tttgaacact
     9481 tccggcgcgt agtacagcac cacattgatg ccgacaaatt gctggaagat ggagagcata
     9541 acgccgatta caatcacgcc cacgccaaac atcagcagac gaccaccggt tttgcggcca
     9601 tgatccaggg agtgtttaat ttcctgtact gcctgagttg caagcgtgtt gcccataatt
     9661 ttgcgcagga tactttccgt ctgttcttgc ttgccgcgtg acatcagcca gcgaggactt
     9721 tctggcacgg tatacagcag cattaagaac agcagtgcag ggatacattc cgaggcaaac
     9781 atataacgcc tgccgtcagt attcagccag ctggcatcac cggaacgggc aataaaatag
     9841 tttacgcagt aaactaaaag ttgcccgaaa ataatcgcaa actggttaaa tgaaaccagt
     9901 tttccgcgaa tatgagctgg agccagttcc gcaatataca ttggtgaaag cattgaggct
     9961 aaaccaacgc caataccgcc aataatacga taaataacaa attccgggac ataacctgcc
    10021 agataaatag gtactgtgtt gtccgggttt atagaggtaa aaccaagttc tggccaggca
    10081 gaacctacac cagaaataaa aaacaggaca gcagaaatct taagtgaatc acgacggccg
    10141 aagcggttac tgcaataacc accgagggca ccgccgatga tgcaaccaat cagagcgctg
    10201 gctacgcaaa atcctaacag ggagttggca gcggattcac ttaagttttg tggagcaaca
    10261 aagacggtat tgagtgactc aacagtaccg gaaataacgg cggtgtcgta gccaaataat
    10321 aaaccaccta atgtagcgac taaggtaatc gaaaatatat aactggaatt atactgggta
    10381 ttcattcaga cctgccttag accattctga tcttttatca gtgcattgat gttggaattg
    10441 tcaggcagtt aaggcaatta tcatttttta caatgcgttt acgtaatgtc ttttctgtga
    10501 tcttaattgt gataattatc aaaaattgat gaaacaatac tattgccgtg actcagagca
    10561 cgaaagagaa ttatcgtaag tgggaaaaca aataacgtaa aaataataag ttctatgatg
    10621 ggaatattaa ccggcgaacg attcagattg cagacgaaag aaaaaaaggc gctccgtgga
    10681 gcgccgaata atagtcacaa gttgggataa tgtaagttga gggtgcagcg gcataacatt
    10741 ggcagaacaa catctttaac ctttcacacc acctgccgtc aggccgttga ccagccagcg
    10801 ttgagccagc aagaagacga tggtgatcgg taatgcagac atcacggcag cggcggcaaa
    10861 gtcaccccac aggtagtttt gcgggttgag gtattgctgc atccccacgg ccagggtgta
    10921 gctgtttacg tcacgcagta acagcgacgc gaccggaact tcagtaatgg cagcgataaa
    10981 cgacaggatg aataccaccg ccagaatcgg tactgacagc ggcaacagga caaggcggaa
    11041 ggcctgccac ggtgtcgcac catccagcgc agccgcttct tccagcgaac tgtcgatggt
    11101 ttcgaaatag cctttgatgg tccagacatg cagcgcaatc ccacccagat acgcgaaaat
    11161 tacgccgccg tgagtattca ggccaatgaa tggaatgtac tcacccagac gatcaaacaa
    11221 cgcatacaac gcgaccagtg aaagtactgc cgggaacatc tggaaaatca gcattccttt
    11281 cagcagcgtc gctttgcctg gaaagcgcat acgggcgaaa gcgtaggcgc aggtggtgga
    11341 gagcgccaca atgccaatcg cggaaatccc agcgaccttt accgagttcc acagccacag
    11401 cagtaccggg aatggcggtg gcgtaatgcg accatcagcc tgttcaacgc taaaacctaa
    11461 cgccagtttc cagtgatccc aggagatttg ctccgggatc aggctaccgg tcgcgaagtt
    11521 tccctgacgc agcgagatag cgacgaccat cagcagcggg aacataatcg ccgcgataaa
    11581 aagtagcagt agcaggtgag taataaacaa acgtgctttt tgcgatttcg gttggaccat
    11641 tgccattttt gttatctccc ttaatcaaac ttcattcgcg tggctttcag gttcactatc
    11701 gccagcgcac ccaccagcag gaagatcagc gtggcaattg ctgccgccag accgaagtcc
    11761 tgacccccgc cgccttcaaa agcgatacgg taggtgtagt taacaagcag gtcggtataa
    11821 ccggctggcg tggtcgtgcc aagacgatcc gggccgccgt tggttaacag ctgaatcagc
    11881 acgaagttgt taaagttaaa ggcgaagctg gcgatcatca gcggcgtcag cggtttaatc
    11941 agcagcggca gcgtaatctt aaagaagttc tggaacggac ctgcgccatc cattgctgag
    12001 gcttcataca aatcgtccgg aatcgctttc agcaagccca tgcagaggat catcatgtac
    12061 ggataaccca gccaggtatt gacgataatc agcatcgtgc gagcggtggt cggatcgctg
    12121 aaccaggcgg gcttcacgcc aaacagcgcg ctcaacatca tgttgatttc accgaagctc
    12181 tggttaaaca atcctttgaa aatcaagatt gaaatgaacg atggcaccgc gtagggcaga
    12241 atcagcagga cgcgatagac cgctttgccg cgcaacgctt cccactgcac cagacatgcc
    12301 agaaccatgc cgaccgccac cgttaaaaag acagtgatca gcgagaacac cacggtccag
    12361 acgaaaatgg cgaggaacgg tttctgaatg ccttcgtcgg taaagacgcg ggtaaagttt
    12421 ttccagccgg tggtcacggt gtaaccgggg cttagctttt catcccccca gttgccgtcg
    12481 gcggtaatgg actggtaaaa gccaatttgg ttattcggac gatatttcac gccactctga
    12541 ttattcgtca acgtgccgtc accgtcgagt gtgtagagcg gctgcgtgcc agaaaactgg
    12601 cgcagggagc tcatcatcac tttgttgcca tccggcagaa tggcggtaat gtcactcagc
    12661 gcctgacgat tctgggtaat cacgcgcaga ttcgcgcgtt cgccttcggg ctgggcggtc
    12721 gtttctttca gttgcagttt ttgctcgccg ccaaatttaa acgcgtcgga gaggtaattt
    12781 ttgccggttt cgccgtcgct gagcgccagt tgccactcat cgcccgccgg gtaaagacca
    12841 aagttatagg ttttgcctgc ttgccaggag cgatctaaca acacttcctg cgcacgttca
    12901 aaagtcagct ggttagtgct gctgtagttg gtgaaggcaa tggcgatggt gcagaccaga
    12961 gggaagagga cgaataatcc cattccagcc attcccgggt aaacatagcg ccaggcgtag
    13021 gctttacgat tggcgaaaat atacagcccc gctgaactca atatcagcgt ggtaatggcg
    13081 aacaggtatt ccccttgtgc gtacattaaa acaacaaggt aacccaccag caggccgagc
    13141 agacctagca ctgaccattt cagcgcgtcg ctttgccacc aatgtttctt tttaatgaca
    13201 tccatggggt tcttcctcat tccaggacgg ataaggcttt cacgccttat ccgacaacaa
    13261 ctgcctgatg cgacactgat gcgtcttatc aggcctacat gcgtttcagt tttgtaggcc
    13321 ggacaaggcg ttcacgccgc atccggcatt tcacagcatt acttggtgat acgagtctgc
    13381 gcgtctttca gggcttcatc gacagtctga cgaccgctgg cggcgttgat caccgcagta
    13441 cgtacggcat accagaaagc ggacatctgc gggatgttcg gcatgatttc acctttctgg
    13501 gcgttttcca tggtggcggc aatacgtgga tctttcgcca actcttcctc gtaagacttc
    13561 agcgctacgg cacccagcgg tttgtcttta ttaaccgctt ccagaccttc atcagtcagc
    13621 agatagtttt cgaggaactc tttcgccagc tctttgttcg gactggcggc gttaataccg
    13681 gtgctcagca cgccaacgaa cggtttagat ggttgaccct tgaaggtcgg cagtaccgtt
    13741 acaccataat tcactttgct ggtgtcgatg ttggaccatg cccacgggcc gttgatggtc
    13801 atcgctgttt cgcctttatt aaaggcagct tctgcgatgg agtaatcggt gtctgcattc
    13861 atgtgtttgt ttttaatcag gtcaaccagg aaggtcagac ccgctttcgc gccagcgtta
    13921 tccacgccca cgtctttaat gtcgtacttg ccgttttcat acttgaacgc ataacccccg
    13981 tcagcagcaa tcagcggcca ggtgaagtac ggttcttgca ggttgaacat cagcgcgctc
    14041 ttacctttcg ctttcagttc tttatccagc gccgggatct cttcccaggt ttttggcggg
    14101 ttcggcagca ggtctttgtt ataaatcagc gataacgctt caacagcgat tgggtaagca
    14161 atcagcttgc cgttgtaacg tacggcatcc catgtaaacg gatacagctt gtcctggaac
    14221 gctttgtccg gggtgatttc agccaacagg ccagattgag cgtagccacc aaagcggtcg
    14281 tgcgcccaga agataatgtc agggccatcg ccagttgccg caacctgagg gaatttctct
    14341 tccagtttat ccggatgctc aacggtgact ttaattccgg tatctttctc gaatttctta
    14401 ccgacttcag cgagaccgtt atagccttta tcgccgttaa tccagattac cagtttacct
    14461 tcttcgattt tggcgagagc cgaggcggaa aacatcatcg tcgttaatgc ggataatgcg
    14521 aggatgcgtg cacctgtttt tattttcata atctatggtc cttgttggtg aagtgctcgt
    14581 gaaaacacct aaacggactc tagtttcttt atacggcaac ctctttccat cctccttgcc
    14641 cctacgcccc accgttgctt tgtgtgatct ctgttacaga attggcggta atgtggggat
    14701 gcgcacataa aatcgccacg atttttgcaa gcaacatcac gaaattcctt acatgacctc
    14761 ggtttagttc acagaagccg cgttctcatc ctcccgcctc ctcccccata aaaaagccag
    14821 ggggtggagg atttaagcca tctcctgatg acgcatagtc agcccatcat gaatgttgct
    14881 gtcgatgaca ggttgttaca aagggagaag ggcatggcga gcgtacagct gcaaaatgta
    14941 acgaaagcct ggggcgaggt cgtggtatcg aaagatatca atctcgatat ccatgaaggt
    15001 gaattcgtgg tgtttgtcgg accgtctggc tgcggtaaat cgactttact gcgcatgatt
    15061 gccgggcttg agacgatcac cagcggcgac ctgttcatcg gtgagaaacg gatgaatgac
    15121 actccgccag cagaacgcgg cgttggtatg gtgtttcagt cttacgcgct ctatccccac
    15181 ctgtcagtag cagaaaacat gtcatttggc ctgaaactgg ctggcgcaaa aaaagaggtg
    15241 attaaccaac gcgtcaacca ggtggcggaa gtgctgcaac tggcgcattt gctggatcgc
    15301 aaaccgaaag cgctctccgg tggtcagcgt cagcgcgtgg cgattggccg tacgctggtg
    15361 gccgagccaa gcgtattttt gctcgatgaa ccgctctcca acctcgatgc tgcactgcgt
    15421 gtgcaaatgc gtatcgaaat ctcccgtctg cataaacgcc tgggccgcac aatgatttac
    15481 gtcacccacg atcaggtcga agcgatgacg ctggccgaca aaatcgtggt gctggacgcc
    15541 ggtcgcgtgg cacaggttgg gaaaccgctg gaactgtacc actatccggc agaccgtttt
    15601 gtcgccggat ttatcggttc gccaaagatg aacttcctgc cggtaaaagt gaccgccacc
    15661 gcaatcgatc aagtgcaggt ggagctgccg atgccaaatc gtcaacaagt ctggctgcct
    15721 gttgaaagcc gtgatgtcca ggttggagcc aatatgtcgc tgggtattcg cccggaacat
    15781 ctactgccga gtgatatcgc tgacgtcatc cttgagggtg aagttcaggt cgtcgagcaa
    15841 ctcggcaacg aaacccaaat ctatatccag atcccttcca ttcgtcaaaa cctggtgtac
    15901 cgccagaacg acgtggtgtt ggtagaagaa ggtgccacat tcgctatcgg cctgccgcca
    15961 gagcgttgcc atctgttccg tgaggatggc actgcatgtc gtcgactgca taaggagccg
    16021 ggcgtttaag caccccacaa aacacacaaa gcctgtcaca ggtgatgtga aaaaagaaaa
    16081 gcaatgactc aggagataga atgatgatta ctctgcgcaa acttcctctg gcggttgccg
    16141 tcgcagcggg cgtaatgtct gctcaggcaa tggctgttga tttccacggc tatgcacgtt
    16201 ccggtattgg ctggacaggt agcggcggtg aacaacagtg tttccagact accggtgctc
    16261 aaagtaaata ccgtcttggc aacgaatgtg aaacttatgc tgaattaaaa ttgggtcagg
    16321 aagtgtggaa agagggcgat aagagcttct atttcgacac taacgtggcc tattccgtcg
    16381 cgcaacagaa tgactgggaa gctaccgatc cggccttccg tgaagcaaac gtgcagggaa
    16441 aaaacctgat cgaatggctg ccaggttcca ccatctgggc aggtaagcgc ttctaccaac
    16501 gtcatgacgt tcatatgatc gacttctact actgggatat ttctggtcct ggtgccggtc
    16561 tggaaaacat cgatgttggc ttcggtaaac tctctctggc agcaacccgc tcctctgaag
    16621 ctggtggttc ttcctctttt gccagcaaca atatttatga ctataccaac gaaaccgcga
    16681 acgacgtttt cgatgtgcgt ttagcgcaga tggaaatcaa cccgggcggc acattagaac
    16741 tgggtgtcga ctacggtcgt gccaacctgc gtgataacta tcgtctggtt gatggcgcat
    16801 cgaaagacgg ctggttattc actgctgaac atactcagag tgtcctgaag ggctttaaca
    16861 agtttgttgt tcagtacgct actgactcga tgacctcgca gggtaaaggt ctgtcgcagg
    16921 gttctggcgt cgcgtttgat aacgaaaaat ttgcctacaa tatcaacaac aacggtcaca
    16981 tgctgcgtat cctcgaccac ggtgcgatct ccatgggcga caactgggac atgatgtacg
    17041 tgggtatgta ccaggatatc aactgggata acgacaacgg caccaagtgg tggaccgttg
    17101 gtattcgccc gatgtacaag tggacgccaa tcatgagcac cgtgatggaa atcggctacg
    17161 acaacgtcga atcccagcgc accggcgaca agaacaatca gtacaaaatt accctcgcac
    17221 aacaatggca ggctggcgac agcatctggt cacgcccggc tattcgtgtc ttcgcaacct
    17281 acgccaagtg ggatgagaaa tggggttacg actacaacgg cgatagcaag gttaacccga
    17341 actacggcaa agccgttcct gctgatttca acggcggcag cttcggtcgt ggcgacagcg
    17401 acgagtggac cttcggtgcc cagatggaaa tctggtggta atagcaaaac ctgggccgga
    17461 taaggcgttt acgccgcatc cggcataaaa acaggttgtc attatctgaa aggggcgaaa
    17521 gcccctctga ttatcgggtt tagcgcgcta ttgcctggct accgctgagc tccagatttt
    17581 gaggtgaaaa caatgaaaat gaataaaagt ctcatcgccc tctgtttatc agcagggtta
    17641 ctggcaagcg cgcctggaat tagccttgcc gatgttaact acgtaccgca aaacaccagc
    17701 gacgcgccag ccattccatc tgccgcgctg caacaactca cctggacacc ggtcgatcaa
    17761 tctaaaaccc agaccaccca actggcgacc ggcggccaac aactgaacgt tcccggcatc
    17821 agtggtccgg ttgctgcgta cagcgtcccg gcaaacattg gcgaactgac cctgacgctg
    17881 accagcgaag tgaacaaaca aaccagcgtt tttgcgccga acgtgctgat tcttgatcag
    17941 aacatgaccc catcagcctt cttccccagc agttatttca cctaccagga actaggcgtg
    18001 atgagtgcag atcggctgga aggcgttatg cgcctgacac cggcgttggg gcagcaaaaa
    18061 ctttatgttc tggtctttac cacggaaaaa gatctccagc agacgaccca actgctcgac
    18121 ccggctaaag cctatgccaa aggcgtcggt aactcgatcc cggatattcc cgatccggtt
    18181 gctcgtcata ccagcgatgg cttactgaaa ctgaaagtca aaacgaactc cagctccagc
    18241 gtgttggtag gaccgctatt tggttcttcc gctccagctc cggttacggt aggcaacacg
    18301 gcggcaccag ctgtggctgc acctgctccg gtgccggtga agaaaagcga gccgatgctc
    18361 aacgacacgg aaagttattt taataccgcg atcaaaaacg ctgtcgcgaa aggtgatgtt
    18421 gataaggcgt taaaactgct tgatgaagct gaacgcctgg gatcgacatc tgcccgttcc
    18481 acctttatca gcagtgtaaa aggcaagggg taattacgcc ccacagtgct gattttgcaa
    18541 caactggtgc gtctcctggc gcaccttttt ttatgcttcc ttcctgggat atgagcgatt
    18601 ttttatagta cttcacttct tcttcactaa gaatgtccat tatttcaatg cattatcaga
    18661 gattcttttc ctttcgccgg tagtgtctgg acgctcaggc tacttttcca ggttatttta
    18721 tttctgtatt acagaggttt tatgataggt catatcacaa attctggcgg caataactct
    18781 ttgatgaaac atgatgtggt gcaaggaaat aatatagtag atcttgattt actacgtaat
    18841 ttcaatgggg tgacaggttt aaacagagat tactttattt atatcagtga tatattttta
    18901 aatataaaac aacggaacga aaaaaatcat gcaataaata tgttacgtga agtctcaatt
    18961 agtaatgata ctataagtgt aaaattctac agaaatgaaa aaatagaatg cgcttgtaat
    19021 tttctgatgg ataaagatgc gcaggggtat atcgacctgt ctgattttga tttaacaaat
    19081 tgtcatttta aaggtgacgt tatttctaag gtgtcttttt tatcatcaaa tctacaacat
    19141 gtaactttcg aatgtaaaga aattgagaat tgcaatttta ctaaggcaac agttgataat
    19201 gtcatattta aatgtcgacg tttacacaat gtgatttttc tcaaaacgag tggcgaatgt
    19261 gttgatttta gccaaaatat tcttgataca gttgacttct cgcagagtca acttggtcat
    19321 agtaattttc gcgaatgtca gattagaaat tcaaacttcg ataattgtta tctttacgct
    19381 tcgcacttca ccagagcaga gtttctgtct gccaaagaaa tatcatttat taaatcgaat
    19441 ttgacagctg ttatgtttga tcatgtgcga atgtcgacag ggaattttta agattgcatt
    19501 acagaacaat tggaattaac tattgattat tcagatatat ttgggaatga agatctcgat
    19561 ggttatatca ataacattat aaaaatgatt gatacattgc cagataatgc aatgatattg
    19621 aaatccgttc tggcagtaaa actggtgatg caattaaaaa ttctcaatat tgttaataaa
    19681 aactttattg agaatatgaa gaaaacattt agccattgtc cttatataaa agatccaatt
    19741 atacgtagtt atatacattc tggggaagat gacaagttcg ataattttat gcgtcaacat
    19801 cgattcagta aggtgaattt cgatacccaa cagatgatcc attttattaa cagatttaat
    19861 atgaataaag ggctggttga taaaaataac aattttttta tccaacttat cgatcaggcc
    19921 ttacgatcaa cggatgatat gatcaaagca aatgcctggt atctttataa agagtggatt
    19981 cgtagtgatg atgtttcacc tatatttata gaaactgaag aaaagttaag aacctttaac
    20041 actaataaat taacacgaaa cgataatata tttatcctgt tctcctctgt cgatgatggg
    20101 cctgttatgg tggtaagctc ccagcgctta catgatatgt tgaatcctac aaaagatacc
    20161 aattggaatt ccacgtgtat ctataaatcc agacataaga tgttgcctat taatcttact
    20221 caggaaacac ttttcagctc caaatctcat ggtaaatatg cgcttttccc aatttttact
    20281 gcgagttggc gagctactcg tataaagaat aaaggtattt aagtaaagga aaacatcacc
    20341 gttcctagca tcatgggcgg tgatgcccct atggttgccc tcgccagcac gggcatcggc
    20401 aaagcgtaag gttcaacagc gttttaccac ttcatgcgat tgttgcgttt ttgttgcgca
    20461 ttagatcact taatttgctt tacatctccc gtaaacactt ttctgcgata caatgccttt
    20521 acgttatgta acggagagtt cggcatgtca caccccgcgt taacgcaact gcgtgcgctg
    20581 cgctatttta aagagatccc tgccctggat ccgcaactgc tcgactggct gttgctggag
    20641 gattccatga caaaacgttt tgaacagcag ggaaaaacgg tgagtgtgac gatgatccgc
    20701 gaggggtttg tcgagcagaa tgaaatcccc gaagaactgc cgctgctgcc gaaagagtct
    20761 cgttactggt tacgtgaaat tttgttatgt gccgatggtg aaccgtggct tgccggtcgt
    20821 accgtcgttc ctgtatcaac attaagcggg ccggaactag cgttacaaaa attgggtaaa
    20881 acgccgttag gacgctatct gttcacatca tcgacactaa cccgggattt tatcgagata
    20941 ggccgtgatg ccggactgtg ggggcgacgt tcccgcctgc gattaagcgg taaaccgctg
    21001 ttgctaacag aactgttttt accggcgtca ccgttgtact aagaggaaaa aaatatggag
    21061 tggagtctga cgcagaataa gctgctggcg tttcatcgct taatgcgtac ggataagcca
    21121 attggcgcgt tactgctgct ctggccaaca ttatgggcgt tgtgggtggc gacaccgggc
    21181 gttcctcagc tctggatcat ggcggtgttt gtcgcgggtg tctggctgat gcgcgctgct
    21241 ggttgcgtgg tgaatgatta tgctgatcgc aagtttgatg gtcatgttaa gcgcacggcg
    21301 aaccgaccac ttcccagcgg cgcggtaaca gagaaagagg cgcgcgcgct gtttgtagtg
    21361 ctggtactga tttcgttttt actggttctg acgctgaata cgatgaccat tctgttgtcg
    21421 attgccgctc tggcattggc gtgggtgtac ccgtttatga agcggtatac ccatctcccg
    21481 caagtggtgc tgggcgcggc gtttggctgg tcgattccaa tggcttttgc cgctgtgagt
    21541 gagtcggtgc cattgagttg ctggttaatg ttcctcgcca atattctctg ggcggtggct
    21601 tacgacacgc agtatgcgat ggttgaccgc gatgatgatg tgaagattgg cattaaatcc
    21661 acggcaatcc tgttcggcca atacgataaa ttgattattg gtattttgca gattggcgta
    21721 ctggcactga tggcgatcat cggtgagtta aatggcttag gctggggata ttactggtca
    21781 attgtggtgg ctggcgcgct gtttgtttat caacaaaaac tgattgccaa ccgcgagcgt
    21841 gaagcctgct ttaaagcatt tatgaataat aactatgttg gtctggtact gtttttagga
    21901 ctggcaatga gttactggca tttctgatga tgtaaaaaag ccggatgatc atccggcttt
    21961 cttctgggtt gcctgatgcg ttgcgcttct caggcctacc cggtgaatcg caatttattg
    22021 aatttgcagg tcatggtagg ccggataagg cgttttcgcc gcatccggca attctctctg
    22081 attacccttc gccctgcgtc gcactctcaa tcgtcaaacg cacgtctgat gtaatcaact
    22141 ccgccagcaa ctgataaacc ttcatcgttt ctgctggttc ggcatcgccg ctatcgctga
    22201 tatacccttc atcacgcagc gtcagcacca gagaactgaa caccgccttg tcgaagaact
    22261 ccggcgcgtt gatgccgtgc agcacggaga gacgttgcgc aacggtgcgg ctctctttct
    22321 ccagcgtacc gcggttgatc gacgggttgg cactcaacaa ccagaaggtg atggcataac
    22381 gttgcagcgt ttcgcgcgcg cctgcggcca gcagctgtag ggtgcgagaa tgcgccgggt
    22441 tgatatgcaa ctcatcatct tgcagggtaa tcagcccctg acgttgcatc tcatttgcca
    22501 gcgcatcaat aacgtccggc aactcgtcgc gatcccagcg caggaacagc tccgctttca
    22561 gcattgggta aagcacattg acgtgctcca tcaatacgtc gcgggagatg tggcgatgct
    22621 gggtgacaat tgccgccatc agcgaaggca gcaccaacat atgcgcaatg ttgttgcgat
    22681 agtaggtcat cagcaccgct tgctcgcgtg gcagaatgat gatgtcgccg atagtgtctt
    22741 tctcgacttc aaacttgttc atttgcagcg cgtgatcgat aagctcgctg gcgctggctg
    22801 aaggaacggt agagtccgtg gagtaaggta cgttgcgcat cagatccagg tagcagttga
    22861 gttgctcggt taactgctcg cgggtgagtg agcgctgacg tgatgccagt agcgcagtac
    22921 agcacaggtt catggcgttt gccgcgcctg cgttgttaat gcgtaccatc agatcggcag
    22981 caatattatt gaccgtcggc gttaaccagg ccggacgcac cgcttcgatg ggatcgatag
    23041 attcacgcca gtctggtacg tgctggttaa ggtaggtcat caacggcatt ggttcaccga
    23101 agttgacgta accctgaccg agattacgca gcttgcttaa accgcgcagc atctgcggca
    23161 ggctctcttt ctctttcgtc gcgccgcgca gttctttggc gtaagtaccc acttccatga
    23221 cgtgctcata accgatatag atcggaatca gcgtaatcgg acgcgtgccg ccacgcagca
    23281 tcgcctgaat ggtcatcgac agcgtaccgg ttttcggatc cagcaaacgc cccgtgcggg
    23341 aacgaccgcc ttccacgaag tactcgacgg aataaccacg gctgaacagt tcgccgagat
    23401 actcacggaa aacggtggaa taaagtttat tgcctttaaa agtacggcga ataaagaacg
    23461 cccccagacg gcggaaaatc ggcccggccg gccagaagtt caggttgatc ccggcggcga
    23521 tatgcggcgg caccagtccc tggtggtaca gcacgtaaga aagtagcagg tagtccatgt
    23581 gactgcggtg gcaaggcaca tacaccagct catgaccgtc gtgggccagc tggcgaacgc
    23641 gctcggcgtt atggacgttg atgccctgat aaaatcggtt ccaggtgaag cccagaatgc
    23701 ggtcagtcag gcggatcatc tcgtaagaga aattcgccgc aatctcttcc atcagcgcaa
    23761 tcgcgttctg ctgcgctttt tcatgggaga tttttttgct gcgcgcttca tcttctaccg
    23821 ctttggcaat ggcgcgggag gcgagcagct tattaaacag atcctgacga gcaggcagac
    23881 gtgggcctac ggcagccaga cgttgacggg caaagtgcat acgcgccacg cgcgccagtt
    23941 tctgagcgat agttttatcc gtgccgtgtt catccgccat acggcgcagc gacactgacg
    24001 gcgagaaacg cacaaaactg tcgcgaccga gccacagtac agcgaaaaat ttctgtacgc
    24061 cgttaagcat acgcagcggc gggttcactt cgcctttttc acgccccggc gcgcgaccaa
    24121 acatcaccga tactggcacc atctgcacat ccagatttgg gttgctacgg tgcaaatcga
    24181 gatagtcgtg gaaaagctta atagactctt ctttcggcgt gtaataggtg aatacacgcg
    24241 gcccgccgtg aatgaacaca tagcgcggca gtagcgtgcc gtcgatttcc agcggctcta
    24301 acgggtcagg caaatcatgt gccagacact gggcgcgcaa cgtcagcaaa tctgctttcg
    24361 agttgtacgg taaaacgtac ataattggac gagaggtatc cagccccagt tccggggcag
    24421 gatctgccgg aatagacttg ctttttacca ggatgcttaa tggtaaattc agtaatttgt
    24481 agtaaattcg tggccagccg gacataaacg atgtaaagcc tctggttaat aatgcaaatg
    24541 cgcggcaagg atagcagaaa gtcatgggaa attctgtggt atccgctcat gtttcgcgcg
    24601 gcgttacgca aacccgaatc atcggattta acggtacact gatattgacg ctcataatgt
    24661 aaaaaggttc tttcaatggc caataataca actggattca cccgaattat caaagctgct
    24721 ggctattcct ggaaaggttt acgcgctgca tggatcaacg aagcggcatt ccgtcaggaa
    24781 ggcgtagcgg tattgttggc ggtggtcatc gcctgctggc tggatgtgga cgcaattacc
    24841 cgcgtgctgc ttatcagctc cgtgatgctg gtgatgattg tggaaatcct caatagcgcc
    24901 atcgaagcag tggttgaccg aattggctct gaataccatg agctttccgg acgcgcaaaa
    24961 gatatgggat ccgccgcggt gctgattgcc attatcgtcg ccgtgattac ctggtgcatc
    25021 ctgttatggt cgcattttgg ataacccttc cagatttcga taaatctctg gtttattgtg
    25081 cagtttatgg ttccaaaatc gccttttgct gtatatactc acagtataac tgtatataca
    25141 cccagggggc ggaatgaaag cgttaacggc caggcaacaa gaggtgtttg atctcatccg
    25201 tgatcacatc agccagacag gtatgccgcc gacgcgtgcg gaaatcgcgc agcgtttggg
    25261 gttccgttcc ccaaacgcgg ctgaagaaca tctgaaggcg ctggcacgca aaggcgttat
    25321 tgaaattgtt tccggcgcat cacgcgggat tcgtctgttg caggaagagg aagaagggtt
    25381 gccgctggta ggtcgtgtgg ctgccggtga accgcttctg gcgcaacagc atattgaagg
    25441 tcattatcag gtcgatcctt ccttgttcaa gccgaatgct gatttcctgc tgcgcgtcag
    25501 cgggatgtcg atgaaagata tcggcattat ggatggcgac ttgctggcag tgcataaaac
    25561 tcaggatgta cgtaacggtc aggtcgttgt cgcacgtatt gatgacgaag ttaccgttaa
    25621 gcgcctgaaa aaacagggca ataaagtcga actgttgcca gaaaatagcg agtttaaacc
    25681 aattgtcgtt gaccttcgtc agcagagctt caccattgaa gggctggcgg ttggggttat
    25741 tcgcaacggc gactggctgt aacatatctc tgagaccgcg atgccgcttg gcgtcgcggt
    25801 ttgtttttca tctctcttca tcaggcttgt ctgcatggca ttcctcactt catctgataa
    25861 agcactctgg catctcgcct tacccatgat tttctccaat atcaccgttc cgttgctggg
    25921 actggtcgat acggcggtaa ttggtcatct tgatagtccg gtttatttgg gcggcgtggc
    25981 ggttggtgca acggcgacca gctttctctt tatgctgttg ctgtttttac gcatgagcac
    26041 caccgggctg actgcgcagg cttatggtgc caaaaatcct caggcattag cccgtgcgct
    26101 ggtgcaaccg ttgctgttgg cgttgggggc cggggcgtta attgcgctgt tgcgtacgcc
    26161 gattatcgat ctggcgctgc atattgttgg cggtagtgaa gcggtcctgg aacaggcgcg
    26221 gcgctttctt gaaatccgct ggttaagcgc tccggcgtcg ctggcgaatc tggtattact
    26281 cggttggtta ctcggcgtgc aatatgcccg tgcgccagta attttgttag tggtcggcaa
    26341 tatcctcaac attgtgctgg atgtctggct ggtgatgggg ctgcatatga acgtgcaggg
    26401 cgcggcgctg gcgacggtta ttgcggaata tgcaacattg ctgattggtc tgctaatggt
    26461 gcgtaaaatc ctcaaactac gcggaatttc cggcgaaatg ctgaaaactg cctggcgagg
    26521 aaacttccgt cgcttgctgg cgcttaaccg cgatatcatg ctgcgttcgc tgttgttgca
    26581 actctgtttc ggcgcgatca ccgtacttgg tgcgcgactg gggagtgaca ttatcgctgt
    26641 taacgcggtt ctgatgacgc tactcacctt taccgcctat gcgcttgatg gttttgccta
    26701 cgcggttgaa gcgcactccg gtcaggcata cggtgcgcgc gacggtagcc agttgctgga
    26761 tgtatggcgg gcagcgtgcc gccagtcggg gattgtagcg ttactatttt cggtggttta
    26821 tttgctggca ggggaacaca tcattgcgtt gctgacgtcg ttaacccaga ttcagcagct
    26881 ggctgaccgc tatcttatct ggcaggtgat tttgccgttg gttggcgtct ggtgttatct
    26941 gctggacggc atgtttatag gcgcaacgcg tgccgccgaa atgcgtaaca gtatggcggt
    27001 ggccgccgca ggttttgcgc tgacgctcct tacgctgccg tggctgggta atcatggttt
    27061 gtggctggca ttaaccgtct ttctggcgtt gcgcgggctt tctctggcgg ctatctggcg
    27121 gcgtcactgg cgcaacggta cctggtttgc cgcaacgtga cggttaaaaa ttctgaataa
    27181 attatcctaa gccaaattgc tgactacact taatctcacg ttcagaagaa aagtgaacgt
    27241 actctcattc acaacctaac gatgaggtct tgattatgaa taaagatgaa gccggcggta
    27301 actggaaaca gtttaaaggt aaagtgaaag agcaatgggg caaactgacc gatgatgata
    27361 tgacgatcat tgaaggtaaa cgtgatcaac tggtcggtaa aatccaggaa cgttatggtt
    27421 atcagaaaga tcaggcagaa aaagaggtcg tggattggga aacccgcaat gaatatcgct
    27481 ggtaattaat ccctcctgcc cgacgtgtac aaggatgtac gccctcttaa cgcggtttct
    27541 ttttcacctg cacagagtga tcatgctggc actgttcagg atgacgacac gcttccactt
    27601 ccacgcatgc cgcacagagc ccatgtgctt caatcacatt atgccgcagg gcaaacccca
    27661 tttttgccgc cagcgtatgc ataatgtctt ccacgccttc tgcacactct tctttcaccg
    27721 cgccgcagcg atcgcaaata aacatggctg acgtatgcgt gggctggtcg aacagatgac
    27781 agagcacata actgttggtg gattccacct tatgcacaaa accttgctca agcagaaaat
    27841 ccagcgcgcg ataaaccgtt ggcggcttgg cttgcggttc agcttcgcgc agtaaatcaa
    27901 gcagatcata agcgctgata gcgccatcct gcagactcat caggcgcaac acttccaggc
    27961 gctgtggggt caggcgcaca ttacgctgcg cgcagatttt ttcagcctgc gctaataact
    28021 cctgcgttgt ggtcttttcc atcgggcacc tcaaagtggg ggttaagaat gtccttactt
    28081 taccatgttc caggaaaaac accgagaacc cttattgttg ccgtaatgtt gattttctgt
    28141 tttgtaggta aggtgttgtg ttgccttatc gtaccattat caacacgata ataattaata
    28201 attcattttt aaataaattg cttcaagtta actcgctatt tatttaactg ggtttaaacg
    28261 catacctgta gttcagaaat tatgagcgat ttttgatagt gaccagatgg caaaagaatt
    28321 ataaggagga cacggtacga caaaaaaggt agatgaatca tgttgaagat aatacccggt
    28381 gcaactggat actttaataa aacactcaat tcaaatcaat tcgaaaataa agatgcaacc
    28441 aaagataaat tggataatag aggttcaatc aaaggtaaat taaataatat atatggtaaa
    28501 tctattgatt atgcagcgct gcgtcatcgg gatataatta ttgcaaaaat agatttgttt
    28561 attcaacgaa tcacgcataa tttatggcat gctcgtaaaa aaatgtgttt ttaattgagc
    28621 acataaatga tctcaaaatg tgggtcaata agtatactga cgattgcact gatgaggatt
    28681 taaacgatcg tgactttatt gcatcagttg ttgaccgggc tatttttcat ttcgcgatta
    28741 atagtatatg taatcctggg gataataaag atgcaatgcc cattgaacaa tgtacttttg
    28801 atgtagaaac taagaatggc cttccctcca cggttcagct attttatgag gaatctaagg
    28861 ataatgaacc tttagcgaat atacattttc aagcaat
//
LOCUS       DAJTSI010000042        28769 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000042 DAJTSI010000000
VERSION     DAJTSI010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28769
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.042"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(163..453)
                     /gene="ubiK"
                     /locus_tag="OO327_001609"
     CDS             complement(163..453)
                     /gene="ubiK"
                     /locus_tag="OO327_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR6018186.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
     regulatory      575..723
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            827..1480
                     /gene="ribB"
                     /locus_tag="OO327_001610"
     CDS             827..1480
                     /gene="ribB"
                     /locus_tag="OO327_001610"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR6018187.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     gene            1741..1911
                     /locus_tag="OO327_001611"
     CDS             1741..1911
                     /locus_tag="OO327_001611"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018188.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(1969..2742)
                     /gene="zupT"
                     /locus_tag="OO327_001612"
     CDS             complement(1969..2742)
                     /gene="zupT"
                     /locus_tag="OO327_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR6018189.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            2885..3673
                     /gene="ygiD"
                     /locus_tag="OO327_001613"
     CDS             2885..3673
                     /gene="ygiD"
                     /locus_tag="OO327_001613"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR6018190.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            complement(3711..4871)
                     /locus_tag="OO327_001614"
     CDS             complement(3711..4871)
                     /locus_tag="OO327_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6018191.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(4877..5548)
                     /locus_tag="OO327_001615"
     CDS             complement(4877..5548)
                     /locus_tag="OO327_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR6018192.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            complement(5696..7177)
                     /gene="tolC"
                     /locus_tag="OO327_001616"
     CDS             complement(5696..7177)
                     /gene="tolC"
                     /locus_tag="OO327_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR6018193.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7382..8011
                     /gene="nudF"
                     /locus_tag="OO327_001617"
     CDS             7382..8011
                     /gene="nudF"
                     /locus_tag="OO327_001617"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR6018194.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            8012..8434
                     /locus_tag="OO327_001618"
     CDS             8012..8434
                     /locus_tag="OO327_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR6018195.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            8459..9286
                     /gene="cpdA"
                     /locus_tag="OO327_001619"
     CDS             8459..9286
                     /gene="cpdA"
                     /locus_tag="OO327_001619"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR6018196.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            9286..9867
                     /gene="yqiA"
                     /locus_tag="OO327_001620"
     CDS             9286..9867
                     /gene="yqiA"
                     /locus_tag="OO327_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR6018197.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            9896..11788
                     /gene="parE"
                     /locus_tag="OO327_001621"
     CDS             9896..11788
                     /gene="parE"
                     /locus_tag="OO327_001621"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR6018198.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(11836..12150)
                     /locus_tag="OO327_001622"
     CDS             complement(11836..12150)
                     /locus_tag="OO327_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR6018199.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(12181..12762)
                     /gene="mdaB"
                     /locus_tag="OO327_001623"
     CDS             complement(12181..12762)
                     /gene="mdaB"
                     /locus_tag="OO327_001623"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR6018200.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            13081..13413
                     /locus_tag="OO327_001624"
     CDS             13081..13413
                     /locus_tag="OO327_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCR6018201.1"
                     /translation="MLKQKIKTIFEALPYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            complement(13459..14733)
                     /gene="qseC"
                     /locus_tag="OO327_001625"
     CDS             complement(13459..14733)
                     /gene="qseC"
                     /locus_tag="OO327_001625"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR6018202.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(14730..15389)
                     /gene="qseB"
                     /locus_tag="OO327_001626"
     CDS             complement(14730..15389)
                     /gene="qseB"
                     /locus_tag="OO327_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR6018203.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            15541..15933
                     /gene="ygiW"
                     /locus_tag="OO327_001627"
     CDS             15541..15933
                     /gene="ygiW"
                     /locus_tag="OO327_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR6018204.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            15986..16468
                     /locus_tag="OO327_001628"
     CDS             15986..16468
                     /locus_tag="OO327_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR6018205.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            17014..19272
                     /gene="parC"
                     /locus_tag="OO327_001629"
     CDS             17014..19272
                     /gene="parC"
                     /locus_tag="OO327_001629"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR6018206.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            19506..20243
                     /gene="plsC"
                     /locus_tag="OO327_001630"
                     /gene_synonym="parF"
     CDS             19506..20243
                     /gene="plsC"
                     /locus_tag="OO327_001630"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR6018207.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            20318..21730
                     /gene="ftsP"
                     /locus_tag="OO327_001631"
     CDS             20318..21730
                     /gene="ftsP"
                     /locus_tag="OO327_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR6018208.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            21841..24060
                     /locus_tag="OO327_001632"
     CDS             21841..24060
                     /locus_tag="OO327_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCR6018209.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTLGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24103..24360)
                     /gene="yqhH"
                     /locus_tag="OO327_001633"
     CDS             complement(24103..24360)
                     /gene="yqhH"
                     /locus_tag="OO327_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR6018210.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(24411..25337)
                     /locus_tag="OO327_001634"
     CDS             complement(24411..25337)
                     /locus_tag="OO327_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR6018211.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            complement(25537..26364)
                     /gene="dkgA"
                     /locus_tag="OO327_001635"
     CDS             complement(25537..26364)
                     /gene="dkgA"
                     /locus_tag="OO327_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR6018212.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            complement(26469..27632)
                     /gene="yqhD"
                     /locus_tag="OO327_001636"
     CDS             complement(26469..27632)
                     /gene="yqhD"
                     /locus_tag="OO327_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR6018213.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            27826..28725
                     /gene="yqhC"
                     /locus_tag="OO327_001637"
     CDS             27826..28725
                     /gene="yqhC"
                     /locus_tag="OO327_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR6018214.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
BASE COUNT         6751 a         7114 c         7658 g         7246 t
ORIGIN      
        1 tatcgattaa gtaaaactta aagaatacat aacaattata ttttcaattg caatcattca
       61 tttttgtgat gatactctga atgggattaa gcgataatgt aatagaaaac actgtaaatt
      121 agaggattaa ttcataattt acagtgcatt aaagacaaat gtttacagcg ttggggggag
      181 agtctctgga tctggctgct ttttgatctc agtactgcga ttttccagtt cgctgattct
      241 ttgttcaagc agcgccagtt tctcacgagt ccgtaacagg acttgcgttt ggacgtcgaa
      301 ctcttcacgg cttacaaggt cgaggcgcgt cagctgagct tgtagggttt ggcggatttt
      361 tttctccaca tcttccccga actccctgat tcctttaggc attgattcgt gaacctggcg
      421 agcgatttgc tcaatttttt tcgggtcaat cattgtagtt tccctgtact gataggtgtt
      481 gagtaccatt gtagtgcgat aagggtaagt cataaaccag aattatgtga agctatgcgt
      541 tgctgccgct aatcattagc gttatagtga atccgcttat tctcagggcg gggcgaaatt
      601 ccccaccggc ggtaaatcaa ctcagttgaa agcccgcgag cgctttgggt gcgaactcaa
      661 aggacagcag atccggtgta attccggggc cgacggttag agtccggatg ggagagagta
      721 acgattctgt cgggcatgga cccgctcacg ttattttggc tatatgccgc cactcctaag
      781 actgccctga ttctggtaac cataatttta gtgaggtttt tttaccatga atcagacgct
      841 actttcctct tttggtacgc ctttcgaacg tgttgaaaat gcactggctg cgctgcgtga
      901 aggacgcggt gtaatggtgc ttgatgatga agaccgtgaa aacgaaggcg atatgatctt
      961 cccggcagaa accatgaccg ttgagcagat ggcgctgacc attcgccacg gtagcggtat
     1021 tgtttgcctg tgcatcactg aagatcgccg taaacaactt gatctgccaa tgatggtaga
     1081 aaataacacc agcgcctatg gcaccggttt taccgtgacc attgaagcgg ctgaaggtgt
     1141 gactaccggt gtttctgccg ctgatcgtat tacgaccgtt cgcgcagcga ttgccgatgg
     1201 cgcaaaacct tctgatctga atcgcccggg ccacgttttc ccacttcgcg cgcaggcagg
     1261 tggtgtactg acgcgtggcg gtcatactga agcgactatt gatttgatga cgctggcagg
     1321 ctttaaaccg gctggtgtgc tgtgtgagct gactaatgac gatggcacga tggcgcgtgc
     1381 accagagtgc attgagtttg ccaataaaca caatatggcg ctcgtgacta ttgaagacct
     1441 ggtggcatac cgtcaggcac atgagcgtaa agccagctga aaaccgctgc ttaattactg
     1501 ccttaatcaa gaaaccgaag ttgtagctgg cttcggtttt tattttgccc tgctatgcaa
     1561 gatgttaatt ccgcttctct ttctatgaga aaatttcatt aatatcaggc attctttttc
     1621 attatgatca aaatcatata attgttccgt tattatgcga ccatatgaat atatccgaaa
     1681 aaggatatgt gaaatttatg cgctaaatgt ttagtattgt taaatattaa gggtaaaagg
     1741 atgtttatcg cctggtactg gattgtattg attgctctgg ttgtggtggg ttatttcctg
     1801 catttgaaac gttattgtcg ggcgtttcgc caggacagag acgcactgct tgaagcgcgg
     1861 aacaaatact taaacagtac gagagaagag acagccgaaa aggtagaata aggctttcgt
     1921 gaagctggca gaagttgtcc atgtgctgga caacttctga tgttgttttt aaccaattcc
     1981 cgccgtttgt agcagcacta aactgaatcc catcactgac attccacata gtacgccata
     2041 gctggggtta ttattagggt caatctcttt ggcgagcggc attaattcat caaccgagag
     2101 cgccaccata attcctgcaa ccgccgccat gattgccgcc atgaccaccg gggaaatcat
     2161 gctaccgagg attaaccacg ccagcacacc accaagaatt tctgctagtc cagaaatccc
     2221 cgcccacaga atcgcggtgc gtttagaccc cgttgccgca taaaccgggc ctgccactgc
     2281 cagaccttca gggatattgt gcaacgcgac ggccagtgcg atgccaaatc ccagctccag
     2341 gttgctgctc gccgtgacaa aggtggcaat cccttccggg aagttatgca gactgatgcc
     2401 gagagtgagc agaatggctg tgcgcttgat cgattttggc aacagctgca ccgatttttg
     2461 cattaaatcc tgcggatgcg catgcggcaa catgcggtcc aggccaaagt agccaagcag
     2521 accgaagata aacattccat aacccaacac cggcgacatt ccttcagctg ctagtgcggc
     2581 aggaagcatt tccattaatg agatgagcaa catgatcccc gccgcaaaac ctagcgaaaa
     2641 cgccagtaag cggttcgagg gtttttgtcc gagaacgccg agaaacgcgc caataaacgt
     2701 ggctgccccc gccagtatgg tcagaatgag aggtactgac atcgacaact ccttataaat
     2761 cttcaccttc aggcagatcg tcaaaataac tgatgatcat catcattgtt atccgggttc
     2821 ttactccgcc gaacaagcgt attgtgagga tatcaaaatg acacctttag ttaaggatat
     2881 catcatgtct tcaacacgta tgccagcatt gtttttaggt cacggtagtc cgatgaacgt
     2941 gctggaagat aatttgtata cccgcagctg gcagaagttg gggatgacat tgccacgccc
     3001 gcaagcgatt gtggtggttt cggctcactg gtttacccgt ggaacaggag tgaccgcgat
     3061 ggagacgccg cccacgattc atgactttgg tggcttcccg caggcgctgt acgatacgca
     3121 ttatcctgct ccgggttcgc ctgcgctggc acagcgtctg gttgagctgt tagcgccgat
     3181 cccggtgacg ctggataaag aagcctgggg ctttgaccac ggctcgtggg gcgtgctgat
     3241 taagatgtat cctgacgccg atatccctat ggtgcagttg agtatcgaca gtagcaaacc
     3301 tgccgcctgg catttcgaaa tggggcgcaa actcgcggcg ctgcgtgatg aaggaataat
     3361 gttggtcgcc agcggtaacg tggtgcataa cctgcgcaca gtgaagtggc acggtgatag
     3421 ttcaccgtat ccgtgggcga cgtcgtttaa tgagtatgtg aaagcgaatc tgacgtggca
     3481 agggccggta gagcagcatc ctctggtgaa ttatctcgac catgaaggtg gcgcgttatc
     3541 gaacccaacg ccagagcact atctgccgtt gttgtatgtg ttaggtgtgt gggatgggca
     3601 ggagccaatt accattccgg tcgatggtat agaaatgggc agtttaagta tgctgtcggt
     3661 gcagataggc taacaattac cggatggcga gtgccatccg gtatcgtggc ttattcaaca
     3721 aaaatatgtg gataaaaccg agacatatcc tgggtgatca acgcacggtc ttcacgaatg
     3781 ccaattccgg caggttgatc gttcaccagc cagctaccaa tcagcatata gctgtcgccg
     3841 aatttcggta acgggtggaa ttgctgaaca atcatccctt cttcgccata cggaccttcc
     3901 gctgcttcaa tggttttgcc gttctcaatg atcgacacgt ttgcgccttc acgggagaag
     3961 atcggtttaa ccacatattt ttccatttgc ggatgatcat cttccgcaaa ataagcgggc
     4021 agcaggttcg ggtgattcgg gaacatctcc cacagtagcg gtagaagtgc cttgttggag
     4081 ataatgctct tccacgccgg ttccagccag cgtacgccag catcttccag cttggtggag
     4141 aacatttcgc gcagcataaa ttcccacgga taaagcttga acaggttaga aatcacctga
     4201 tcctgtaaat ccgtgaactg gcctttttcg cctaaaccta tctcgtcgat gtagaggaac
     4261 tcggtggcga tttcagcttc ggttgcgcag tcctgcaaat actgaatggt tccgcgatct
     4321 tccaccgtgt cgcgacagca ggtgagatgc agcaactgga agccatactg ttcacgcagc
     4381 tcaacgaagc gatcgatcag tttttcttgc agactgttaa actggtcgct gccctccggc
     4441 aagttaccgg cgttaagctg atcttccagc cagatccact gaaagaacgc cgcctcgtat
     4501 agtgacgttg gcgtatcggc gttattttcc agaagtttag gttcgccagt gccatcccac
     4561 gccagatcaa gacgcgaata aagcgatggc tggtgagtca gccatgattg acgcacaaaa
     4621 ctccaggtgt gttttggtat gcggaatttg gtcatcagct catcgctggc gatcactttt
     4681 tccaccactt tcaggcacat ctggtgcagt tcggcggtga cttcttccag cttttcaacc
     4741 tgggcgaggg tcaacttgta gtaagcatct tcacaccagt acggctcgcc gtacatggtg
     4801 tgaaaattga aaccgtattc gtgggctttc tcacgccagt ccgggcgctc ggtaatactg
     4861 actctttcca tcggtatcag ccacccattg aacgagaaga ggtgccggtt gcgctacgct
     4921 gcatagtgct ttgtttggca acagattcac caaaaccgcc acgggtaacg gtagtggtgg
     4981 tcgccggttt tggtgccatt gccgtcttcg gtacggtcat ggtgcggcct ggctgggctg
     5041 cgccatagtt tttacccgtc gcgtcggtat atttaccgta agccggactg gccgggtttt
     5101 tcgaggagaa cagcggctgc tgtgcaaatc ccgcgccgcc gcccatcaga cgccccatca
     5161 tgtaaccggc catcagcggc atccagaaac tcccgctgga ttgctgagcc tgcgcctggt
     5221 tttctggtgc catgccagcc tgtgctggtg cttgctgaca ctgaccttca ccaaattcag
     5281 caacacagtc ttcacgggtg gcgtatttcg gcgcagtacg ttcggcttct ttcagcgcat
     5341 tgttgtacgc ggtggtacat tcggcgcttt tgcctgggtt tgcagctgaa cagtcgtcag
     5401 cattttgata gagagacact gtttcatcac tcttttcaca gccagccagc ataaaaacag
     5461 tggcaaccgc gagagcgact ggtgtcagat ggcgtgcgct ccagtttttg cggaacgatg
     5521 cgtggcgtat ggattttgtc cgtttcattt ttgtcttccg ggaccagtgg taaataccca
     5581 tcagaataga ggatggctgg tcgaaattga agcgagaagg gggaagaatg cggcagataa
     5641 cccgtatctt tacgttgcct tacgttcaga cggggccgaa gccccgtcgt cgtcatcagt
     5701 tacggaaagg gttatgaccg ttactggtgg tagtgcgtgc ggatgtttgc tgaacgacgg
     5761 gtgccgggct atcaggcgca taaccatcag caatagcatt ctgttccggc gtttgcgggg
     5821 caacgttttc cggattagtg gaaaccggtt tgctcagcgc attgttcagt gccagcaaat
     5881 cctgctcgtt caacgtaccc agggctgact taatattcag ctgattaatc aggtagttat
     5941 aacgcgcatt cgccagctct tgcttggcgt tgtacagcgt ggtggtcgca tccaacacat
     6001 caacaatggt acgcgtaccg accgagtagc ccgcttccat cgcgtctaat gagctttgcg
     6061 cggaaactac ggcttgtttg taggcgttaa tactactgat agaagcatta atgttgttga
     6121 aggaggaacg tacggtttgc acgacgctac gatgtgcgct ttccagttgc tcactggcac
     6181 caacaaagtt gtactgtgcc tgtttcacct gcgagttaac cattccgccc tgataaatcg
     6241 gcagcgagaa gctcaggcca actttgttct ggcccatatt gctatcgtca tactgggtac
     6301 cagcggcacc acgggttttc gaaccgctat aagaggtgtc agaaatcccg gtagaagccg
     6361 ttaaatccag agtcggtaag tgaccatcct gcgcctggcg aatttgctcg cgctccaggt
     6421 cctggctcaa gcgtgcctgt aacagcgaca ggttgcgttt ttcggcttct ttcagcagcg
     6481 cgttaaccgg ctgtggtttg tcggttttaa agttttcgac attcagcgcc gccagttccg
     6541 gatagtagtt accggtgatc tggcgcagct gctctaccgc gttatcaagg ttattacgtg
     6601 cggtcacttc gttcgccagc acggtatcgt actgcgcgcg ggcgttctgc acgtcggtga
     6661 tcgctaccag gcccacgtta aaacgttggg tggtttgatc taattgacgg tagatcgctt
     6721 ctttttgtgc ctgtgtatag gaaagaacgt caatagcatt caacacgttg aaataagcgg
     6781 tcgcggtgtt gaggatcaag gtttgctgat cggtctgata tgtgacgtcc tgaatccctg
     6841 ctgctttttc ctgcagcgtt aacgcacgcc atttcgacat atcaaaaatg gattgagtta
     6901 actgcaggga cgcactggtc gcgttcgagt tgatgccgtt cgcgtcgcgg tagccgttgc
     6961 tataggtgta atctgcacct aaacctagct gtggcagtaa tggactgcgc gcttcattaa
     7021 ttttttcaaa ggcagcatca cgatcggcgg cagacttacg caattccggg ttactaaggc
     7081 gtgcttgctg ataaacttgc atcaggttct cggcctggct caacgaactg aacccagaaa
     7141 ggctcaggcc gataagaatg gggagcaatt tcttcatttg cattccttgt ggtgaagcag
     7201 tatttagcgc gatcaaactg taaaattatt gcggattcta gcagaagccg ctaccgcaaa
     7261 aggttggcgt tacgtgccat agggcgttaa tgtgcctcaa tttaacatat gactgctcaa
     7321 acggcagtca aacgtcgctg aaattcacat ttaattcact attagtgcta ggacattaac
     7381 aatgcttaag ccagacaacc tgcccgttac atttggcaaa aacgatgtag aaattattgc
     7441 acgagaaaca ctttatcgcg gctttttttc attagatctt tatagatttc gtcatcgtct
     7501 attcaacggg caaatgagtc atgaggtacg gcgggaaatt tttgagcgcg gtcacgccgc
     7561 agtcttgcta ccctttgacc cagtgcgtga tgaagttgtg ctgattgagc agattcggat
     7621 tgccgcgtac gacaccagcg aaaccccctg gctactggag atggttgccg ggatgattga
     7681 agagggtgaa agtgtggaag atgttgcccg tcgcgaagcg attgaagagg cgggactgat
     7741 agtcaaacgg accaaaccgg tgttaagttt cctggcaagc ccggggggca ccagtgagcg
     7801 ttcgtcaatt atggtgggcg aagtggacgc cacgaccgca agcggtattc atggtctggc
     7861 tgatgaaaac gaagatattc gcgttcatgt ggtaagccgg gaacaggcat accagtgggt
     7921 agaagagggg aaaatcgaca acgcagcgtc ggtcatcgct ttgcaatggc tgcagctgca
     7981 tcatcaagcg ttaaaaaatg agtgggcata aatgaagcgt tacacacctg actttcctga
     8041 aatgatgcgc ctgtgcgaga tgaacttttc acaattgcgc cgtttgttac cgcgcaatga
     8101 cgcacccggc gaaactgtaa gctatcaggt ggcaaacgca caatatcggc tgacgattgt
     8161 ggaatcgacc cgatacacta ccctggtgac aatagaacag actgcgcccg cgatcagtta
     8221 ctggagcctt ccgtcaatga cggtgcgtct gtatcatgac gcgatggtgg ctgaagtgtg
     8281 ttcaagccag cagatttttc gcttcaaagc gcggtatgat tatcctaata aaaagttgca
     8341 tcaacgcgac gaaaagcatc aaattaacca gtttttagcg gactggttgc gatactgttt
     8401 agcacatgga gcgatggcga ttccggttta ctagcgtcgt gaaacctaag gacaccattt
     8461 ggaaagcctg ttaacccttc ctctggctgg tgaggccaga ggaaggattt tacaaattac
     8521 cgatactcac ctgtttgcac aaaagcacga agccctgtta ggggtaaaca cctgggaaag
     8581 ctaccaggcg gtgctggagg cgattcgtcc acaccagcac gaattcgacc tgattgtcgc
     8641 gacaggtgat ttagcgcagg atcaatcctc tgcggcctat cagcatttcg ctgaaggcat
     8701 cgcaagtttt cgtgcgccct gcgtctggct gccgggcaac cacgatttcc agcccgcgat
     8761 gtacagcgcg ttacaggatg cgggtatctc cccggcgaag cgggtgttta ttggtgagca
     8821 atggcaaatc ctgttgctgg atagccaggt gtttggcgtg ccgcacggtg agctgagcga
     8881 gtttcagctt gagtggctgg aacgtaaact ggccgatgcg ccagaacgcc atacgttgct
     8941 gctgctgcat catcatccgc tacctgcggg ttgtagttgg ctcgatcaac acagtctgcg
     9001 taacgcgggc gaactggata ccgtgctggc gaagtttccg cacgtcaaat acttgctgtg
     9061 cggtcatatt catcaggagc tggatctcga ctggaacggt cgccgcctgc tggcaacgcc
     9121 gtcgacctgt gtgcagttta agccgcactg ttccaacttt acgctggata ctatcgcgcc
     9181 cggctggcgt actctcgagt tacatgctga tggcacgctg accaccgagg tgcatcgcct
     9241 ggcggacaca cgtttccaac ctgataccgc ttcagaaggc tactgatgtc tacgcttctt
     9301 tatttacacg gtttcaacag ctcgccgcgc tctgcaaaag cgagcttgtt aaaaaactgg
     9361 ctggcggaac atcaccctga cgttgagatg atcattccgc agttgccgcc gtatccttcc
     9421 gacgcggcag aactgctgga atccattgtc ctggaacatg gcggcgattc gctgggtatt
     9481 gtcggttcgt cactgggggg atattacgcc acctggttgt cgcaatgttt tatgctgccc
     9541 gcagtggtgg taaacccggc ggtacgcccg tttgaattgc tgacggacta tctcggtcag
     9601 aacgagaacc cctacaccgg gcagcaatat gtgctagagt cacgccatat ttacgatctc
     9661 aaagtcatgc agattgaccc gctggaagcg ccggatttga tctggctgct gcaacagacg
     9721 ggagatgaag tgctggatta ccgccaggcg gtggcgtact atgcttcctg ccgccagact
     9781 gtcatagaag gcggcaacca cgcattcacg ggcttcgaag attatttcaa cccgatcgtc
     9841 gattttcttg gtctgcacca tctctgacga tcaactcgaa ttgctaactt aaaccatgac
     9901 gcaaacttat aacgctgatg ccattgaggt actcaccggg cttgagccgg ttcgccgccg
     9961 tccggggatg tataccgata ccactcgccc taaccatttg gggcaagaag ttattgataa
    10021 cagtgtcgat gaagcactgg cgggtcacgc aaaacgcgtg gatgtaatct tacatgctga
    10081 ccagtcgtta gaagtgattg acgatgggcg cgggatgccg gtggatattc acccggaaga
    10141 aggagtaccg gcggttgaac tgattctttg ccgtctgcat gcaggcggta aattctctaa
    10201 caaaaattac cagttctctg gcggcctgca tggcgtgggg atttcggtag ttaacgcctt
    10261 gtcgaagcgc gtagaagtta acgtgcgccg cgatggtcag gtttataaca tcgcctttga
    10321 aaatggcgaa aaggtgcagg atttacaggt tgtcggcact tgcggtaaac gcaataccgg
    10381 taccagcgtg cacttctggc cggatgaaac cttctttgac agcccgcgtt tttctgtttc
    10441 acgcctgacg catgtgctga aggccaaagc agtactgtgc cccggcgttg agattacctt
    10501 taaagatgag atcaacaaca ccgaacagcg ctggtgctat caggacggtc tgaatgatta
    10561 cctggcggaa gcggtaaacg gtttaccgac gctgccagaa aaaccgttta tcggtaattt
    10621 cgctggcgat actgaagctg tggactgggc gctactgtgg ctgccggaag gcggtgaact
    10681 gctgaccgaa agctacgtca accttatccc aacgatgcag ggcggtaccc atgttaatgg
    10741 tctgcgtcag ggcctgttgg acgcgatgcg tgagttctgt gaataccgca acattctgcc
    10801 gcgcggcgtg aagctgtcgg cggaagatat ctgggatcgc tgcgcctatg ttctgtcggt
    10861 taaaatgcag gatccgcagt ttgccgggca gaccaaagag cgtctctctt cgcgtcagtg
    10921 tgcggcattc gtttcgggcg tggtgaaaga tgccttcatc ctgtggctga accagaacgt
    10981 tcaggcggcg gagttgctgg cagagatggc gatttccagc gcccagcgtc gtatgcgtgc
    11041 ggctaaaaaa gtggtgcgta aaaagctgac cagcggcccg gcgctgcctg gcaaactggc
    11101 tgattgtacc gcgcagggcc ttaaccgtac cgaactgttc ctcgtggaag gtgactccgc
    11161 aggcggatct gccaagcagg cgcgcgatcg tgaatatcag gcgatcatgc cgctgaaagg
    11221 taagatcctt aacacctggg aagtttcttc cgacgaagtg ctggcctcgc aggaagtgca
    11281 tgatatttcg gtagcgatcg gtatcgatcc tgacagcgac gatctgagcc agcttcgtta
    11341 tggcaaaatc tgtatcctcg cggatgcgga ctctgatggt ctgcacattg ccacgctgct
    11401 ctgcgctttg ttcgtaaaac acttccgcgc gttggtgaaa cacggtcacg tttacgtcgc
    11461 actgccaccg ctctaccgta ttgatctcgg gaaagaggtt tattacgcgc tgacggaaga
    11521 agagaaagag ggcgtacttg agcaattaaa acgcaagaaa ggcaagccga acgtccagcg
    11581 ttttaaaggt ctgggggaaa tgaacccgat gcaattgcgt gaaaccacgc ttgatccgaa
    11641 cactcgccgt ctggtgcagt tgactatcga tgatgaagac gatcagcgta ctgacgcgat
    11701 gatggatatg ctgctggcga agaaacgctc ggaagatcgc cgcaactggt tgcaagagaa
    11761 aggcgacatg gcggagattg aggtttaaag gaaagaacat tgcccggtca ggatggccgg
    11821 gcaaacaagg caggattaaa tccctggctg cagaatacgg atattcatct ccagcacgtc
    11881 gccttttacg gcttcgctgt acgccttcat gtgcggggtt tgcagatgcg cttcaaggtg
    11941 cgcgatgctt tcccactgct caatcatcac gatagaatcc ggtgccatag actggaaact
    12001 cacgccagca gcgcaatcca ccattggcgc atagccgtgg caaccttctt ctttcagtac
    12061 ggttggaacg attttagcaa actgatccaa taccgcctga cggtgatgtt gaccaggacg
    12121 agtacggatt tctgcgatta cggtaagcat ggttaactcc ttctaaagcc tgagctctag
    12181 ttaaccaaaa atttccacaa gatgcttgcg atattcttca gtatagcggg gaacatcagg
    12241 catttttatc acgtcattag cgataaatgt cggcagcggt tccataccga ggaattggtt
    12301 tgctttatgg aacggcagat acacaccgtc aacgccaacg ccgtggaaga actgatcttt
    12361 ttcggtgaag gcttccattg gtgcgttcca ggttagagaa agcatatatt ttttgtcctg
    12421 taccaggctg ccggaaccgt attttttcga cggatctttg cgggtacgac catcgctggc
    12481 atacagagtc ccgtgacctt cggtgaatac atcatcaata tattttttca ctgtccacgg
    12541 cgcgcccatc caccagcctg gcatctgcca gatcaccaca tcagcccaga gaaagttttg
    12601 tacttccgct ttgacattgt agtcgctgtc ggcgcgaacg atgcggacat catgcccgag
    12661 gtcgcgcagt gtgccatccg cgacttcggt cagggtgtcg ttcagttgac cattggagtg
    12721 ggcgaatttt ttcgcgccgt tgataatcag gatgttgctc attttttaat ctcaaggtga
    12781 gacgtttgtc gactatagta cgtcacagag cggtactgtg aaattagcaa aatgtgcaaa
    12841 gtcttttgcg aatttagcaa aagtctgatc tagctctgac tcaccgatgc aggataaata
    12901 ttttctgaaa atgacaatac tgatagccat tcctcataat tcttactcgc accggaataa
    12961 tttacacgtt tggttcggtt tttttgggga ttgaaaatat ggctttgaat aagtaaaaga
    13021 tgaaaatatc aacgtgtcat gttgataatt ataaatggat ttttatgagc gtactgatat
    13081 atgttaaagc agaaaattaa aacaatattt gaagcattgc catatataat gcttacttat
    13141 tggttaatag attctttttt tgctttcaat aagtatgact ggatgcttga gtcgggaggc
    13201 aatatttgca gtataccttc cgtttcaggc gaagatcgta tattgcaagc aatgattgca
    13261 gcattttttc ttcttacgcc acttattatt cttatcttaa gaaaactctt tatgcgggaa
    13321 atgttcgagt tttggctata tgtattctcg ctcgttattt gtctggtttg tggttggtgg
    13381 cttttttggg gacggtttat attttgctat tagtaaaatc tatgagagtc gttttaacgg
    13441 ctctcataga cagagaagtt accagcttac cttcgcctca aatccacctt gttccgcatt
    13501 cccaaactca acattcatgt catgcagctt ggcgattcgc tggacaatcg atagcccaag
    13561 tccactgccg gtagcggttt gtccgggtgg gcgatagaag cgttcgccaa ttcgcgccag
    13621 tgcctctggt gtcacaccgg ggccgttatc cagcaaattt cgcaccaaca aacttagcaa
    13681 tagcggttgt ccggtgcgtt tgatgccgtg ggcattgagt gtcagtcgca cgtcaatttt
    13741 cgcctgctgc gccgtgtggt aaatatccat caccgacgat tgtaggagat cttcaagcgg
    13801 gatctccgcg acgtcctgaa ggttatccag tgagtccagc cgcgatagcg tgagcagttg
    13861 atcaaccaga cgagtagcgc gatcgatccc ggaatgtaat tggagcagtg cttttttccg
    13921 cgcctgcgga tcatcgtcag agagctgcgc aacttcggtt tgcactttca gcgctgttaa
    13981 cgggctacgt agttcgtgag ctgcatcgga ggtaaagcgt cgttctcgaa ccatcatcgc
    14041 atgtgtgcgg gcgaacagtt gatttagcga ctcaaccagt ggccgcactt cgctgggtac
    14101 gccagtcgcg tttagtggtt tttccgagtc agggtcacgc atacgtagtg ccagcgccag
    14161 tttgttcagc ggcgcgagtt cacgacccag tagtaccatc atgatgatta acataatcgg
    14221 cagtgcgacc agccacggga tcaattgccc ggcaacaatc gccagcgcca tgtcttcacg
    14281 gtattcccat tcctggccaa caacgatgcg atatttgcca tcaggtgagg tcatccagac
    14341 aaaacgccaa ggatcgtctt caccgaccag ttgcccgtca gcaaaacctt cccgttgata
    14401 gctataggga atatcttctc cgttatcgcc atcattaagg accattctgc cgtcgtgggt
    14461 aaagatggca aaggtcagcg catcgtcatc aacatgaccg tgttttaatt tatttggcgt
    14521 ctgtgccatg cgatccgccg cgttgatttc gttgagatcg agcgtactta accgtttggc
    14581 aaacagcatc agttgggtgt cgaacaattc atcgacgtta tccgttgttt gtttccaggc
    14641 gacaaagctg gaaagcagcc aggtcaccga ggccagaatt aaaaagatta gcgtcagcct
    14701 gacgcgcaga ctaagacgtt gggtaaattt catttctcac ctaatgtgta gccaatacca
    14761 tgcacggtac gaataaaatc actaccgagt ttgcgtcgca gatgatgcac atgcacttca
    14821 acggcattac tggtgacctc ttcgtcccag gtatacagtt tctcttcaat cagtttgcgc
    14881 ggcagtaccc gaccagcgtt acgcatcagt aattccagca gggcaaattc ttttggtttc
    14941 agtgttaagg gttcgccagc cagcgtggcg atacgtttgc cggggtcgag catgacgtta
    15001 ccgtggcgca gctcgttgct ggcctggccg ttggttcggc gcatcagagc ctccagcctg
    15061 gcggcgactt ctatcaacgc aaaaggttta cacagataat cgtcagctcc cagacgcagc
    15121 ccttctacac gttccgctaa cgcatcacgc gcggtcaggt tcagtaccgg ctcacgctga
    15181 cctttctctc gccattcgcg caaaatatcg cgaccatcca tgcctggtaa ggttaaatcc
    15241 aggatcaccg catcataagg tgcgctataa agcgcctctt ttccctgacg accttgtgta
    15301 aaccagtcga cactaaaacc cattttacta aggccagttt tgatgccgtc accaatcagc
    15361 atgtcatctt ctatcagtaa aattcgcatt ttttcatccc tgcgataacc gtatttgctg
    15421 ccagtaaacc gtgttgtaac gcgctctgta cagcattaaa ttttattttt ttcccttaag
    15481 aagttgttaa ggactatctt gttaaatgct cggaaacaga cattaaaggg agtaataaac
    15541 atgaaaaaat tcgcagcagt aatcgcagta atggccctgt gcagtgcacc ggtgatggca
    15601 gcagagcagg gcggtttttc tggcccatcg gtaacgcaaa gtcaggccgg aggatttcag
    15661 gggccgaacg gcagcgtaac gactgtagaa agcgcaaaat ccctgcgtga cgacacctgg
    15721 gtaaccctgc gcggcaatat cgttgaacgc atctctgacg atctctacgt gtttaaagat
    15781 gccagcggta ctatcaatgt tgatatcgat cacaaacgct ggaacggtgt gacggtgacg
    15841 ccgaaagata cggttgagat tcagggtgaa gtcgataaag actggaattc tgttgaaatt
    15901 gacgtcaaac agatccgcaa agtaaatccg taattgttac cgctcccggg acgcgttccc
    15961 gggaataatt tcgcagggag gcaaaatgac aaacctgaca ctggatgtaa acattatcga
    16021 tttcccatca atccctgtgg cgatgttgcc gcaccgctgt agccctgagt tgctcaacta
    16081 cagtgtggcg aaatttatca tgtggcgtaa agagacgggg ctttctcctg ttaaccaaag
    16141 ccagactttt ggcgtcgcct gggacgcccc tgccaccacc gcaccggaag cgtttcgctt
    16201 tgatatctgc ggcagcgtta gcgaaccgat tcccgataat cgttatggtg tgagcaatgg
    16261 tgaacttacc ggtggacgtt atgccgtggc ccgccacgtt ggcgagctgg acgatatttc
    16321 acacacgata tggggcatta ttcgccactg gctgcctgca agcggcgaga aaatgcgtaa
    16381 agcaccgatt ctgtttcact acaccaatct tgccgaaggg gtgacagagc agcgactgga
    16441 aacggatgtt tatgtgccgt tggcgtgatg gggaatcttt accggagtgt gagccaaatc
    16501 actcgcgata agtttgagcg aacaatgtca caggcgtatt gttgttgggc ttataatttc
    16561 tccggtaagg aataccatgt atacgcgaaa tttattatcc ggtgcgccgt aagccgtctc
    16621 ggggatatcc atgagcaagc cgcacttatg gcaggaggcg cggggcactg gtggagacac
    16681 agctaaactg ggctgaaccg tatacttaca gtcgactact ttgatgcttt aggcttccct
    16741 ggactcgcat aacctcaact tctcgaatcg cccggagcgg acccccatgc cgagtggtgt
    16801 ggcatgggcg caatcttgtg ctgccctcta tgccgatcaa gtctacagca aagagttgta
    16861 tatcaaggca cattgatgcc tttatcgccg tcggagcacg tccggcggcg ctattcacgc
    16921 tgatctcctg tgactcgacg cggcagataa tgtagtatct ccggcaatat tgcccctttg
    16981 aaggctggcg aataagttga ggaatcagaa ttaatgagcg atatggcaga gcgccttgcg
    17041 ctacatgaat ttacggaaaa cgcctactta aactactcca tgtacgtgat catggaccgt
    17101 gcgttgccgt ttattggtga tggtctgaaa cctgttcagc gccgcattgt gtatgcgatg
    17161 tctgaactgg gcctgaatgc cagcgccaaa tttaaaaaat cggcccgtac cgtcggtgac
    17221 gtactgggta aataccatcc gcacggcgat agcgcctgtt atgaagcgat ggtcctgatg
    17281 gcgcagccgt tctcttaccg ttatccgctg gttgatggtc aggggaactg gggcgcgccg
    17341 gacgatccga aatcgttcgc ggcaatgcgt tacaccgaat cccggttgtc gaaatattcc
    17401 gagctgctat tgagcgagct ggggcagggg acggctgact gggtgccaaa cttcgacggc
    17461 actttgcagg agccgaaaat gctacctgcc cgtctgccaa acattttgct taacggcacc
    17521 accggtattg ccgtcggcat ggcgaccgat attccaccgc ataacctgcg tgaagtagct
    17581 caggcggcaa tcgcattaat cgaccagccg aaaactacgc tcgatcagct gctggatatc
    17641 gtgcaggggc cggattatcc gactgaagcg gaaattatca cttcgcgcgc cgagattcgt
    17701 aaaatctacg agaacggacg tggttcagtg cgtatgcgcg cggtgtggaa gaaagaagat
    17761 ggcgcggtgg ttatcagcgc attaccgcat caggtttcag gtgcgcgcgt actggagcaa
    17821 attgctgcgc aaatgcgcaa caaaaagctg ccgatggttg acgatctgcg cgatgaatct
    17881 gaccacgaga acccgacccg tctggtgatt gtgccgcgtt ccaaccgcgt ggatatggat
    17941 caggtgatga accacctctt cgctaccacc gatctggaaa agagctatcg cattaacctc
    18001 aatatgatcg gtctggatgg tcgtccggcg gtgaaaaacc tgctggaaat cctctccgaa
    18061 tggctggtgt tccgtcgcga taccgtgcgc cgccgactga actatcgtct ggagaaagtc
    18121 ctcaagcgcc tgcatatcct ggaaggtttg ctggtggcgt ttctcaacat cgacgaagtg
    18181 attgagatca ttcgtaacga agatgaaccg aaaccggcgc tgatgtcgcg gtttggcctt
    18241 acggaaaccc aggcggaagc gatcctcgaa ctgaaactgc gtcatcttgc caaactggaa
    18301 gagatgaaga ttcgcggtga gcagagtgag ctggaaaaag agcgcgacca gttgcagggc
    18361 attttggctt ccgagcgtaa aatgaataac ctgctgaaga aagaactgca ggcagacgcg
    18421 caagcctacg gtgacgatcg tcgttcgccg ttgcaggaac gcgaagaagc gaaagcgatg
    18481 agcgagcacg acatgctgcc gtctgaacct gtcaccattg tgctgtcgca gatgggctgg
    18541 gtacgcagcg ctaaaggcca tgatatcgac gcgccgggcc tgaattataa agcgggtgat
    18601 agcttcaaag cggcggtgaa aggtaagagt aaccaaccgg tagtgtttgt tgattccacc
    18661 ggtcgtagct atgccatcga cccgattacg ctgccgtcgg cgcgtggtca gggcgaacca
    18721 ctcaccggca aattaacgtt gccgcctggg gcgaccgttg accatatgct gatggaaagc
    18781 gacgatcaga aactgctgat ggcttccgat gcgggttacg gtttcgtctg cacctttaac
    18841 gatctggtag cgcgtaaccg tgcgggtaag gctttgatca ccttaccgga aaatgcccat
    18901 gttatgccgc cggtggtgat tgaagatgct tccgatatgc tgctggcaat cactcaggca
    18961 ggccgtatgt tgatgttccc ggtaagcgat ctgccgcagc tgtcgaaggg caaaggcaac
    19021 aagattatca acattccatc ggcagaagcc gcgcgtggcg aggatggtct ggcgcaactg
    19081 tacgttctgc cgccgcaaag cacgctgacc attcatgttg ggaaacgcaa aattaaactg
    19141 cgtccggaag agctacagaa agtcactggc gaacgtggac gccgcggtac gttgatgcgc
    19201 ggtttgcagc gtatcgatcg tgttgagatc gactctcctc gccgtgccag cagcggtgat
    19261 agcgaagagt aatatgccgg ggagggcgct gtttcctccc gctagcaagg aacgccggat
    19321 gaaacgttaa gtcgtcatcc ggcagcgtat tcaacccccg gaaacgataa aaattccctt
    19381 gaaaccgttg tttattcatg cgttgcgatt aacaatacgc ttttccagag agcggctttt
    19441 aacaatgccc taacctgatt tcaggtgacg tacaatgcca gtctctcaga ccttcagagg
    19501 gtgttatgct atatatcttt cgtcttatta ttaccgtgat ttacagcatc ttagtctgtg
    19561 tattcggctc catttactgc cttttcagcc cgcgtaaccc gaaacatgtg gccacctttg
    19621 ggcatatgtt tggccgtctt gcgccactgt tcggcctgaa agttgagtgc cgcaaacctg
    19681 cagatgctga aagctacggc aatgctatct atatcgctaa ccaccagaac aactatgaca
    19741 tggtgacagc atcgaacatt gtgcaaccgc cgacggtgac ggtaggtaaa aagagcttgc
    19801 tgtggatccc cttcttcggg cagttgtact ggttaaccgg caacttattg atcgacagaa
    19861 acaatcgcac taaagctcac ggcaccattg cggaagtcgt gaatcatttc aaaaaacgcc
    19921 gtatttccat ctggatgttc ccggaaggaa cccgcagccg tggtcgcggc ctgctaccgt
    19981 tcaagactgg agcatttcac gcggcaattg cggcgggcgt cccgattatt cccgtgtgcg
    20041 tctctacaac ttcgaataag attaatctta atcgactgca caacggtctg gtgattgtcg
    20101 aaatgctgcc gccaattgac gtcagtcagt atggcaaaga tcaggttcgt gagctggctg
    20161 ctcattgtcg ttcgataatg gaacaaaaaa tcgccgagct cgataaagaa gtcgcagaac
    20221 gcgaagccgc cggaaaagtt taagtcggca atctgtattt ttgcggggaa cactttcctg
    20281 cacggtatta ctttagccag ttttacatgg agcaaatatg tcactcagtc ggcgtcagtt
    20341 cattcaggca tcggggattg cactttgtgc aggcgctgtt cccctgaagg ccagcgcagc
    20401 cgggcaacag caaccgctac ccgttccgcc gctacttgaa tctcgccgtg ggcaaccgct
    20461 gtttatgact gtacaacgtg cgcactggtc atttacgcca gggacacgcg cgtcggtctg
    20521 gggaatcaat ggtcgttacc tggggccgac tatccgcgtc tggaagggcg acgatgttaa
    20581 gcttatttac agcaaccgcc tgacagaaaa tgtctcaatg acggtggccg ggctacaggt
    20641 accaggcccg ctgatgggcg gtccggcacg gatgatgtcg ccaaacgctg actgggcacc
    20701 cgtactgcct attcgccaga acgcagctac tctgtggtat cacgccaata ctcccaaccg
    20761 cacggctcag caggtctata acggccttgc cggaatgtgg ctggtggaag atgaagtcag
    20821 caagtcgctg cctatcccca accattatgg tgtggatgat tttccggtca ttatccagga
    20881 taaacggctg gataactttg gtacgccaga atacaacgaa ccgggaagcg gcggctttgt
    20941 tggtgatacg ctgctggtta acggtgtaca aagcccgtac gttgaagtct cgcgtggctg
    21001 ggtgcgcttg cgactgctga acgcgtcgaa ctctcgtcgc tatcaactac agatgagcga
    21061 tggtcgcccg ttacatgtga tttctggcga tcagggattc ctgcctgctc ctgtatcggt
    21121 gaagcaactt tcgctggcac cgggcgagcg ccgcgagatt ctggtggata tgagcaacgg
    21181 cgatgaagtg tcgatcacct gtggtgaagc ggcgagcatt gttgatcgta ttcgcggctt
    21241 ctttgagcca tccagtattc tggtttctac cttggtgcta acgctgcgcc caaccggcct
    21301 tctgccgctg gtcacagaca gtcttccgat gcgcttgctg ccaactgaaa tcatggctgg
    21361 ttcgccaatt cgcagtcgcg atatcagtct gggtgatgac ccgggtatta atggacagct
    21421 gtgggacgtc aaccgtattg atgtcaccgc gcagcaagga acgtgggaac gctggacggt
    21481 acgcgcggac gagccgcaag cgttccatat tgaaggcgta atgttccaga tccgtaacgt
    21541 gaatggcgcg atgccgttcc cggaagacag aggctggaaa gataccgttt gggttgacgg
    21601 acaagtggag ctgcttgttt atttcggtca gccttcctgg gcgcacttcc cgttctactt
    21661 caacagtcag acgctggaaa tggcggaccg tggctcgatt gggcaactgt tggtcaatcc
    21721 ggtaccgtaa tcttcatact cgcccgagga gggcgagtat ggaatacctt tcgatttctc
    21781 tttcgttccg gcttataatc cccggccttt gattattttt ttatcatttc tggaagcaaa
    21841 atgagctcta tctccctgat ccaaccggat cgcgacctgt tctcctggcc gcagtactgg
    21901 gccgcctgtt ttggaccggc accgtttttg ccgatgtctc gtgaagagat ggatcaactt
    21961 ggctgggata gctgcgacat cattttggtt actggcgacg cgtatgtcga tcacccaagc
    22021 ttcgggatgg cgatttgcgg tcgtatgctg gaagcgcagg gctttcgcgt cgggatcatc
    22081 gcccagccgg actggagcag caaagacgac tttatgcgtc tgggtaaacc gaatctgttt
    22141 tttggtgtta ctgctggcaa catggattcg atgatcaacc gctataccgc cgatcgccgt
    22201 ttacgtcatg atgatgccta cacgccagat aatgtcgcgg gtaagcgacc ggatcgcgcc
    22261 acgctggttt atacccagcg ttgtaaagag gcgtggaaag atgtgccggt gatcctcggc
    22321 ggtattgagg ctagcctgcg ccgtaccgcg cattatgatt actggtccga taccgtgcgc
    22381 cgttccgttc tggtagattc gaaagccgac atgctgatgt ttggtaacgg tgaacgtccg
    22441 ctggtggaag tagcgcatcg tctggcgatg ggcgagccga ttagtgaaat ccgcgatgtg
    22501 cgtaataccg caattatcgt aaaagaggcg ctgcctggct ggagcggtgt ggattccacc
    22561 cgtcttgata ccctagggaa aatcgaccca atcccgcatc cgtatggcga agatttgccg
    22621 tgtgcggata acaaacctgt tgcgccgaaa aagcaggaag cgaaagctgt aatcgtgcag
    22681 ccaccgcgcc agaaaccgtg ggaaaaaacc tacgtattgc tgccttcttt cgagaaagtg
    22741 aagggcgata aagtgctgta cgcccatgct tcgcgcattc tgcaccacga aaccaaccca
    22801 ggctgcgccc gcgcattgat gcaaaaacac ggtgaccgct atgtgtggat caacccgcct
    22861 gctatcccgc tttctaccga agagatggac agcgtttttg cgctgccgta caagcgcgtg
    22921 ccacatccgg cctatggcaa tgcccgtatt ccggcttacg aaatgatccg tttttcggtc
    22981 aacattatgc gcggctgctt tggcggctgt tctttctgtt ctattaccga gcacgaaggg
    23041 cgcattattc agagccgttc ggaagattcg atcattaatg agatcgaagc gatccgcgac
    23101 accgttccag gttttacggg cgtgatttcc gatctcggtg ggccaactgc caacatgtat
    23161 atgttgcgct gcaaatcgcc acgcgctgaa caaacttgtc gccgtttgtc gtgcgtttat
    23221 ccggatattt gtccgcacat ggacactaac cacgaaccga cgatcaacct ctatcgccgt
    23281 gcgcgtgatc tgaaaggcat taaaaaaatc cttatcgcct caggcgtacg ttatgacatc
    23341 gccgtggaag atccgcgcta tatcaaagag ttggcgaccc atcacgtcgg cggttatctg
    23401 aagattgctc cggaacatac cgaagaaggg ccgttgtcga agatgatgaa gccgggcatg
    23461 ggcagctatg accgctttaa agagctgttc gatacctact cgaaacaggc aggtaaagag
    23521 cagtatctga tcccgtattt catctctgcg caccccggta cgcgtgatga agatatggtg
    23581 aatttggcgc tgtggctgaa aaagcatcgt ttccgtctcg accaggtaca gaacttctac
    23641 ccatcgccgc tggcgaactc aaccaccatg tattacaccg ggaaaaaacc gctggcgaag
    23701 attggttata agagcgaagg cgtcttcgta ccgaagggcg acaaacagcg tcgtttgcat
    23761 aaagcgttgt tgcgttacca cgatccggca aactggccgt taatccgcca ggcgctggaa
    23821 gcgatgggca aaaagcatct gattggcagc cgtcgcgatt gcttagtgcc tgcgccaacc
    23881 attgaagaga tgcgtgaagc tcgtcgccag aaccgcaata cccgtccggc gttgactaaa
    23941 catacgccga tggcgactca gcgtcagacg cctgctacgg caaaaaaagc gtcgtctacg
    24001 caatctcgtc cggtgaatgc tggtgcgaag aaacgcccta aagcggcggt tggacgttaa
    24061 aaaaaatccc cggcaactga catgctgccg gggatttttt tatcattctg cgtacatacg
    24121 caagcagcgc aggcaatcaa aatagtcctg tgcatcaaga cgcgtattgg ctctgttagc
    24181 ttcggatttg gcagcataga tttgtgggcg tagtgctttc atatcctgct caagccgggc
    24241 gattttggca tttaatgttt gtacattgct cgccagttga ttgacctttt gctcattgac
    24301 gcagccactg agcaagcagg ttgccagaac aacagctccc acggtgaaaa tcgttttcat
    24361 atagtgttcc cttttattta ttgttccggc taattgttat ttaattcagc tcattgcttt
    24421 aatctggcgg cggtttttgc ctcaatctgc ttaagtaggc tgccgctgtt cgggcggata
    24481 aaatcagcga tttcccactt accttcgcgc cgctgtaaaa ccagctcttg ctcttcattg
    24541 ttgccattgg tcagcgtaaa acgaacatga atgcgggcgg aatcggcggt tttctccagg
    24601 actcgaattt catcaacgcg tgaaaatagc gactcttcgc caaaacagat cggattgtcg
    24661 taaataaatt gccgttctat ttgcatatct tcatcgggat tattggccgt cttgaaggtg
    24721 gttttataga aggcattaac cgcctggcgg taagagtcgg aaaccaccca cgtccacggc
    24781 cagctataat cagcaatatg ttcaaagagt tcagcaacaa aggattctgg ctgttctttc
    24841 tgcaacgaag ctaaagcggc cagcgttttt tcattctcgc tattaactgc ttgtaagacg
    24901 ctgccatgat tgctgacaat atcatcaatg acccaacgac cattttccgc caccattttc
    24961 agtgtctgtg tggtcttttc tttatcatct ttaaagatac gaaagcgcac aacggcatcg
    25021 gcatgatcga cgtcagtttg ggttatcgct acgctttcta acaccagatc gccaaaatcc
    25081 tgacaatcac aaaccggatc ataatccagc cagccaatat tgcccggtag tgtaagattg
    25141 tcgttcaggg taagcgcctg ttgaatacgc gctgaagtta tcgcccgctc tccggtttca
    25201 ccgaaataag gcgtactggc atctgattta tagttctgat aaatctgccg gactgtttgt
    25261 tctacggtct gtgaaggagg ctgcgcgtgt acggtaaaac ttgccagggc tgctaaaaac
    25321 agaagtatta ttttcatctg tttttcacct ctccgttgcg tttatcccgg atatagatga
    25381 gttgttcttt taagagcttc cggctctgca tgatgatgtc cttatatttg gcattcctga
    25441 acaaggaaga gtaacaacgg gcgggacgcg aggggaataa atgatttctg aaaagtccgg
    25501 tagcggaaca ttaccgccac cgggagaatt tgcacgttag ccgccgaact ggtcaggatc
    25561 gggaccgaga cgcttgccct gatcgagttt tgcaattttg ccgagttcgt ctttgtcgag
    25621 acggaaatcc cagacatcaa agttttcggc aatacgtgaa ggtgtgaccg atttcgggat
    25681 caccaccagg ccgctatcca gatgccagcg gataacaatc tgcgccgggg ttttgccgta
    25741 tttatctgcc agatcgcgaa tgactttctg atcgaaaacg cctttccctc cttgcgctaa
    25801 tgggctccag gattcggtct ggattttgtg tgtcgcgttc caagcgtgta gctggcgttg
    25861 ttgcatcagc ggatgaagtt cgatctggtt tatcacaggc gtcacgccag tttcatcaat
    25921 caggcgttgc aggtgatgga tctggaagtt gcacacgccg atgcttttga ttaatccctc
    25981 tttttgcaat tcgatcatgc ctttccatgc ttcgacataa tggtcgatag cgggaacggg
    26041 ccagtgcatt aagtagaggt cgatataatc aagctggagt tttttcaggc tgtcgagcag
    26101 ggcttcgcgg gggcgcttgt ggtcgtcgtt ccacagctta gtggtgatga acagttcttc
    26161 tctgttgact gaggcatttt tcagggcttt gccgacacct tcttcgttct tgtaggccgc
    26221 ggcggtatca atcgagcgat aacccacttc taacgctttt tgaatggcgg tgattacttc
    26281 ctcattactt gcttgccaga cgcccagtcc cagctggggc atgacattgc catcctgtag
    26341 cttaataacg gttggattag ccatacgttc ctcctttata tgaactcacc ggagcaggct
    26401 ccggtgaggt gttgtgaact taagtctgga cgaaatgccc gaaaacgaaa gtttgaggcg
    26461 taaaaagctt agcgggcggc ttcgtatata cggcggctga catccaacgt aatgtcatga
    26521 ttttcgccca gttgggtcat gccgtgctct tccagttttt tcagcaaagc cgggatggag
    26581 ctgccgtcca gaccgtagtc ggagaggtgg gtcggcacgc ctaattgctc aaagaaattg
    26641 cgggttgcgg caatcgcggc gtcaatacgc tcatcgtcgg aaccttcagt gatgttccag
    26701 acgcgttcag catattgcag cagcttagcg cgcttggtct cgcgtttttc attccacagt
    26761 gcaggcagga cgatagccag tgtttgcgcg tgatccagac cgtgcatcgc agtcagttcg
    26821 tggcccagca tatgcgttgc ccagtcctgc ggtacgccag tgccaatcaa accgttcagc
    26881 gcctgagtcg ccgcccacat gacgttggcg cgcacatcgt agttttctgg ctctttcagg
    26941 gctttcggac catcttcgat tagcgtcagc aaaatgcctt ctgcgaaacg gtcctgaatt
    27001 ttggcatcaa ccggtttggt aacatactgt tccacggtgt gtacaaaggc gtccactacg
    27061 ccgttagcca cctgacgcgg cggcagggtg taggtataaa ccggatcgag cacggcaaat
    27121 accggctgaa catgtgcaga atggaacgcc tgcttgtcgc ctgtggtttt acgggagatc
    27181 accgcgcctg cgttggattc tgaaccggtt gctggcagcg tcagcacaca gcccatcggg
    27241 atggcgcttt taatctcttt accgcccgtt tgcagaatgt gccacggatc gatattttcc
    27301 ggatagttag ccgctgcggc gataaatttg gtgccgtcca gtacagaacc gccgccaacc
    27361 gccagcagga aagtcacttt ctgttcgcga accagtttca cggcgttcat cagcgtttca
    27421 taagccgggt ttggctcaat accgccaaat tccagcacgt ccatgccttt cagggcatcc
    27481 agaacttgat cgagaacgcc ggtttttttc acgctgccgc caccgtaggt aatcaatacg
    27541 cgagcatcgt gaggaatttg ttcgcgtaaa ccagcgattg cgcctttacc aaacagaatg
    27601 cgggttgggg tgtgcagatt aaagttgttc attacttgct ccctttgctg ggccaatatg
    27661 agggcagaga acgatctgcc tgatgttttt cattgtgatc gccagcgccc tggctctcaa
    27721 tgctcatttc tgccaatgtc ttgcctattt ctccagagtg ctggagaaat gctacaaaat
    27781 tgcgcacaat caaattgccg cattattcct aagaaattac gcgatatgaa acgtgaagag
    27841 atttgtcgct tgctggcgga taaagttaat aaactgaaaa ataaagaaaa tagtttgtca
    27901 gaactgttgc ccgatgtgcg tttgttgtat ggcgagacgc ctttcgcacg tacaccggtg
    27961 atgtacgagc ctggcatcat aattctcttt tccgggcata aaatcggtta tatcaatgaa
    28021 cgcgtgtttc gttatgatgc caatgaatac ctgctgctga cggtgccgtt gccgtttgag
    28081 tgcgaaacct atgccacgtc agaggtgccg ctggcagggt tgcgtctcaa tgtcgatatt
    28141 ttgcagttac aggaactgtt gatggacatt ggcgaagatg agcatttcca gccgtcgatg
    28201 gcagccagcg ggattaactc cgccacgtta tcagaagaga ttttatgcgc ggcggagcgg
    28261 ttactcgacg tgatggagcg accactggat gcgcgtattc tcggcaaaca gatcatccgc
    28321 gaaattctgt actacgtgct gaccggacct tgcggcggcg cgttactggc gctggtcagt
    28381 cgccagactc acttcagtct gattagccgc gtgctgaaac gaattgagaa taaatacacc
    28441 gaaaacctga gcgtcgagca actggcggca gaagccaaca tgagcgtatc ggcgttccac
    28501 cataatttta agtctgtcac cagcacctcg ccgttgcagt atttgaagaa ttaccgtctg
    28561 cataaggcgc ggatgatgat catccatgac ggcatgaagg ccagcgcagc agcgatgcgc
    28621 gtcggctatg aaagcgcatc gcaatttagc cgtgagttta aacgttactt cggtgtgacg
    28681 ccgggggaag atgcggcaag aatgcgggcg atgcagggga attaaatacg ttcccggtac
    28741 agcagccggg aacggggaaa atcgtcagg
//
LOCUS       DAJTSI010000043        28420 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000043 DAJTSI010000000
VERSION     DAJTSI010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.043"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            564..1334
                     /locus_tag="OO327_001638"
     CDS             564..1334
                     /locus_tag="OO327_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR6018215.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1325..1798
                     /locus_tag="OO327_001639"
     CDS             1325..1798
                     /locus_tag="OO327_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR6018216.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1905..2444
                     /gene="dnaT"
                     /locus_tag="OO327_001640"
     CDS             1905..2444
                     /gene="dnaT"
                     /locus_tag="OO327_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR6018217.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2447..3184
                     /gene="dnaC"
                     /locus_tag="OO327_001641"
     CDS             2447..3184
                     /gene="dnaC"
                     /locus_tag="OO327_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR6018218.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3233..3727
                     /locus_tag="OO327_001642"
     CDS             3233..3727
                     /locus_tag="OO327_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR6018219.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3967..6258
                     /gene="opgB"
                     /locus_tag="OO327_001643"
     CDS             3967..6258
                     /gene="opgB"
                     /locus_tag="OO327_001643"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR6018220.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6396..7418)
                     /gene="lgoD"
                     /locus_tag="OO327_001644"
                     /pseudo
     CDS             complement(6396..7418)
                     /gene="lgoD"
                     /locus_tag="OO327_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7557..8471
                     /locus_tag="OO327_001645"
     CDS             7557..8471
                     /locus_tag="OO327_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6018221.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8686..10047
                     /locus_tag="OO327_001646"
     CDS             8686..10047
                     /locus_tag="OO327_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6018222.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10096..11760)
                     /gene="tsr"
                     /locus_tag="OO327_001647"
     CDS             complement(10096..11760)
                     /gene="tsr"
                     /locus_tag="OO327_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCR6018223.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11879..12325)
                     /gene="hpaR"
                     /locus_tag="OO327_001648"
     CDS             complement(11879..12325)
                     /gene="hpaR"
                     /locus_tag="OO327_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR6018224.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12597..13886
                     /locus_tag="OO327_001649"
     CDS             12597..13886
                     /locus_tag="OO327_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR6018225.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALDNDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13883..15349
                     /gene="hpaE"
                     /locus_tag="OO327_001650"
     CDS             13883..15349
                     /gene="hpaE"
                     /locus_tag="OO327_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR6018226.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15351..16202
                     /gene="hpaD"
                     /locus_tag="OO327_001651"
     CDS             15351..16202
                     /gene="hpaD"
                     /locus_tag="OO327_001651"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR6018227.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16212..16592
                     /locus_tag="OO327_001652"
     CDS             16212..16592
                     /locus_tag="OO327_001652"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR6018228.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16660..17463
                     /gene="hpaH"
                     /locus_tag="OO327_001653"
     CDS             16660..17463
                     /gene="hpaH"
                     /locus_tag="OO327_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR6018229.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLETGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17474..18277
                     /gene="hpaI"
                     /locus_tag="OO327_001654"
     CDS             17474..18277
                     /gene="hpaI"
                     /locus_tag="OO327_001654"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR6018230.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18299..19654
                     /gene="hpaX"
                     /locus_tag="OO327_001655"
     CDS             18299..19654
                     /gene="hpaX"
                     /locus_tag="OO327_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR6018231.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCPTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19664..20554
                     /gene="hpaA"
                     /locus_tag="OO327_001656"
     CDS             19664..20554
                     /gene="hpaA"
                     /locus_tag="OO327_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCR6018232.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            20581..21518
                     /locus_tag="OO327_001657"
                     /pseudo
     CDS             20581..21518
                     /locus_tag="OO327_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21745..23303
                     /gene="hpaB"
                     /locus_tag="OO327_001658"
                     /pseudo
     CDS             21745..23303
                     /gene="hpaB"
                     /locus_tag="OO327_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23321..23833
                     /locus_tag="OO327_001659"
     CDS             23321..23833
                     /locus_tag="OO327_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR6018233.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            24226..26376
                     /gene="btsT"
                     /locus_tag="OO327_001660"
     CDS             24226..26376
                     /gene="btsT"
                     /locus_tag="OO327_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR6018234.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26426..26629
                     /locus_tag="OO327_001661"
     CDS             26426..26629
                     /locus_tag="OO327_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR6018235.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26640..27596
                     /gene="yjiA"
                     /locus_tag="OO327_001662"
     CDS             26640..27596
                     /gene="yjiA"
                     /locus_tag="OO327_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR6018236.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27665..>28420)
                     /locus_tag="OO327_001663"
     CDS             complement(27665..>28420)
                     /locus_tag="OO327_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HCR6018237.1"
                     /translation="YTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTE
                     DFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQH
                     ADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQA
                     LVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFY
                     LTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6954 a         7426 c         7128 g         6912 t
ORIGIN      
        1 cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt
       61 catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca
      121 aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa
      181 atacttaatg aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc
      241 cgcagaatgc gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt
      301 gttaacacta aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata
      361 aattcattat agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgctt
      421 tttatcaccg agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta
      481 ccgcaaaaca accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc
      541 acgtctatga caaagggcga gttatgcaaa ctgagcaaca gcgagccgta acgcggcttt
      601 gtatccagtg tggattattt cttttgcaac atggtgcgga aagcgcgttg gttgatgagc
      661 tttcctcacg actgggtcgg gcgctgggaa tggacagcgt cgaaagttct atctcttcga
      721 acgccatagt gctgacaact attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc
      781 acgatcgcgg cattaatatg catgtggtga ctgaagtcca gcacattgtg attcttgcgg
      841 agcatcatct gctggattac aaaggcgtag agaaacgatt tagccaaatt caaccattac
      901 gttacccaag atggctggta gccttaatgg ttggcctttc ttgcgcctgt ttctgtaaac
      961 tcaataacgg tggctgggat ggtgccgtca tcaccttctt tgccagtacg accgcgatgt
     1021 atatccgcca gctgctggca caacgtcatc ttcatccaca gatcaacttt tgccttaccg
     1081 ctttcgccgc caccaccatt tccggattgc ttttgcaact ccccactttc agcaataccc
     1141 ccaccattgc gatggccgcc agcgttctgc tgctagtgcc gggctttccg ttgattaatg
     1201 ccgtcgccga tatgtttaaa ggccacatca ataccggact ggcacgctgg gcgatcgcca
     1261 gtctgctgac actggctacc tgcgtcggcg tagtgatggc actgacgatt tgggggctac
     1321 gcggatgggt gtgatcgaat ttctgttagc gttggcgcag gatatgatcc tcgccgccat
     1381 tcctgcggtc ggctttgcga tggtgttcaa cgttcccgtg cgggcgttac gctggtgtgc
     1441 gctgcttggc tcgataggtc atggttcccg aatgatcttg atgaccagcg ggttgaatat
     1501 tgagtggtca acctttatgg cttctatgct ggtcggtacc attggtattc aatggtcgcg
     1561 ctggtatctg gcgcatccga aagtgtttac cgtggcggcc gttatcccta tgttcccggg
     1621 catatcggct tataccgcaa tgatttcgtc ggtaaaaatc agccagttag gttacagcga
     1681 accgttgatg attaccctgt taaccaactt tcttacagct tcatcgattg ttggtgcgtt
     1741 atccatcggt ctttccattc ctggattatg gttgtaccgc aagcgccctc gcgtataaaa
     1801 ttgcctcgct taagcacacg gatgagagac agcctcctct cctccgtgtg ttactataaa
     1861 agttatctcc cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt
     1921 tgaccccgga cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa
     1981 aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtca
     2041 cgcctgcacg cctggctgaa ctgctggcgc tggaagaaaa gctagcgcgt ccgggaagcg
     2101 atgtcgctct ggacgatcaa ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca
     2161 tggggaaatt cgccatgtat ccggactggc aacccgatgc cgattttatc cgcctggcgg
     2221 cgctatgggg cgtggcgcta agagagccgg tgaccaccga agaactggcc tcattcattg
     2281 cctactggca ggcggaaggt aaagtctttc accatgtgca gtggcaacaa aaactggcgc
     2341 gcagcctgca aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg
     2401 tcagtgaacc tgacagccaa attccaccag gattcagagg gtaacgatga aaaacgttgg
     2461 cgacctgatg caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cattcaaaac
     2521 gggtgaagag ctactggcct ggcagaaaga acaaggggcg atccgctccg ccgctctcga
     2581 acgtgaaaat cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcgtccact
     2641 gcatcagaac tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt
     2701 aagcaaagcg cgccagtatg tcgaagagtt tgacggcaac atcgccagct tcatcttttc
     2761 tggtaagcca ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct
     2821 acgcggtaaa tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac
     2881 cttcaggaat agcggtacca gcgaagaaca actgcttaac gatctgagca acgttgatct
     2941 gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa
     3001 ccagatcgtc gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa
     3061 tatggaagag atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa
     3121 cagtttatgg gtgatcttca actgggatag ctaccgtagc cgggtaacgg gtaaagagta
     3181 ttaatccccg tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa
     3241 aactattaaa catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt
     3301 gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa
     3361 cagcacgaca caggaaggcg gttggtcgct cgcgtcatta actaacttgc ttagcagcgg
     3421 aaaccaagcc ttaagcgcag ataacatgaa caatgccgca ggcattctgc aatactgcgc
     3481 gaagcaaaag ctggcttcgg taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa
     3541 gctgggcctg aacagtgaag agcaaaaaga agacaccaac tatctggacg gtattcaggg
     3601 tttgctgaaa acaaaagatg gtcagcaact caatctggat aacatcggaa cgactccgct
     3661 ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat
     3721 ttcctgaacc gcgaaatgtt acccggctga cgcggctctc tcctgccctt ttccctgctc
     3781 ctggacggtt ttccccctgt tacacgggaa gcgaccgcga tcaaaaaagt ctgtttataa
     3841 actaattcct aacaacatcg catttttttg acactagaat tgcagcaata tggcgacgcc
     3901 attacattgt gcttctcaaa aagcttaaac gttggctaaa cggcccggtt tagtgaggat
     3961 cgcccattgt cagaactact ctctttcgcc ctttttctcg cctctgtgct gatttacgca
     4021 tggaaagcgg gacgtaacac ctggtggttt gcagccacgt taacggtgct ggggctattt
     4081 gtcgttttaa atatcaccct gtttgccagc gactatttta ctggcgatgg tattaacgac
     4141 gcggttctct ataccttaac caacagcctg accggtgctg gcgtcagcaa atacatcctg
     4201 ccgggtatcg gcattgtgct ggggctgacg gcggtgttcg gtgcgctggg ctggatcctg
     4261 cgccgtcgtc gtcatcatcc gcaccatttt ggttacagcc tgctggcgct cttactggcg
     4321 ctgggttcgg tggacgccag cccggcattt cgtcagataa cggaactggt gaaatcccag
     4381 tcacgcgatg gcgacccgga ctttgcggct tattataaag agccgtcgaa aactatccct
     4441 gacccgaaac tcaacctggt ttatatctac ggcgaaagtc tcgagcggac ctattttgat
     4501 aacgaagctt tcccggatct cacgcctgaa ctgggcgcgt tgaaaaatga aggcctggat
     4561 ttcagccaca cgcagcagct gccaggaacg gattacacga ttgcgggcat ggtggcttct
     4621 cagtgcggca taccgctgtt cgcccccttt gaaggcaacg cctccgcctc tgtctccagc
     4681 ttcttcccgc agaacatctg tctgggcgat atcctgaaaa actcgggtta tcagaactat
     4741 ttcgtgcagg gcgcaaatct gcgttttgcc ggtaaagatg tgttcctgaa atcgcacggc
     4801 ttcgaccact tatacggctc agaagagctg aaaagcgtgg tagccgaccc gcactatcgc
     4861 aacgactggg gattctacga cgataccgtt ctcgatgaag cgtggaaaaa gtttgaagag
     4921 ctttcccgct caggtcagcg attctcactg tttaccctga cagtcgatac ccatcacccg
     4981 gatggtttta tctcgcgcac ctgtaaccgc aaaaaatatg attttgacgg taagccgaat
     5041 cagtcattca gcgcggtaag ttgcagccag gagaatatcg cgacgtttat caacaaaatc
     5101 aaagcgtcac cgtggtttaa agatactgtc atcgtcgtct cttctgacca tttagcgatg
     5161 aacaacacgg cgtggaaata cctcaataag caggatcgca ataacctgtt ttttgtcatt
     5221 cgtggcgaca agccgcagca aaagacgctg gcagtgaagc gtaacacgat ggataacggc
     5281 gcgacggtgc tggacattct cggtggcgat aactatctcg gacttggtcg tagcagttta
     5341 tccgggcagt cgatgtcgga aatcttcctc aatatcaaag agaaaacatt ggcgtggaag
     5401 ccagatatca tccgcctgtg gaaattccct aaagagatga aagagttcac catcgaccag
     5461 cagaaaaaca tgattgcctt ctcgggtagc catttccgtt tgccgctgct gttgcgggtt
     5521 tcagacaaac gcgtggaacc gctgccggaa agcgaatact cagcaccgct gcgtttccag
     5581 ctggccgatt tcgctccacg cgacaatttc gtctgggttg acagttgcta caagatggca
     5641 caactctggg ctccggaact ggcactttcc accgactggt gtgtctcgca ggggcagctt
     5701 ggcggtcagc aaattgttca gcatgttgac aaaacaacat ggaagagcaa gacggcattt
     5761 aaagatacgg tcatcgacat ggcgcgttac aaaggcaatg tcgatacgct gaagattgtt
     5821 gataacgata ttcgctacaa agccgacagt ttcatcttta acgtcgccgg tgcgccagaa
     5881 gaggtgaaac agtttagcgg gatttcccgt ccggagtcgt ggggccgctg gtccaacgcg
     5941 cagctgggcg atgaagtaaa aatcgagtac aagcatccgc tgccgaagaa atttgacctg
     6001 gtgattaccg ccaaagcata cggcaataac gccagccgtc ctattccggt acgcgtaggc
     6061 aatgaagaac aaactcttgt gctgggcaat gaagtgacca ccaccacact gcatttcgat
     6121 aacccaaccg atgccgacac actggtaatt gtgccgccgg aacctgtctc aaccaacgaa
     6181 gggaatatcc tcggacactc gccgcgtaag ctcgggatcg gcatggtgga aattaaagtg
     6241 gtagaacgtg aagggtaatt cctgactgat gtgtaggtcg catagggtgc gacctacacg
     6301 ccttctccgg cttacaacac attattaaga aacaaaaaaa acagcgccca acacttcccc
     6361 ggacgctgca tggcaacttt aataatctcg ccttatcaga aagtaattac acccttaatc
     6421 aactcacgat tgttaatcac atcgcgctcg taggtttctg ccagcgtggc gaacggatag
     6481 cgatgggtta acatcatgtc ggcagtgatt ttcccttccg ccatcagtcg accgacttta
     6541 gcgaaatctt ccggcgtggc gttgcggctg cccatcatcg tcgtttcttt tttatggaat
     6601 tccggatcgg agaactgcaa ctcaccttta aacagaccga caaataccac cgtgccgccg
     6661 tgacgaatca gattcacggt attattcatc gcatgttgat tacctgtcgc gtcgatcact
     6721 ttctgcgcca gcgaaccacc aaactgcgcc cgcagctgcg cgtcaaaatc ttcggctgac
     6781 gggtccagca cgggcaactc cagacgcgtt gccacatgtt cacggcgcgc cggactggta
     6841 tccgccacca ccacctgtgc gccatcggct ttagcgattg ccgccgcgcc cagaccgatt
     6901 ggccccgccc cgaccaccag cacctgctcg ccgggagcaa tggctgcgcg acgcaccgca
     6961 tgagcgctaa tagcgaaagg ttcaatcaat gcggccgcct gcgggtcaat accgtctgcg
     7021 ggcaaaatgt tcgccaccgg cacgctcaaa tactcactaa aaccgccatc ctgatgcacg
     7081 ccaatgactg aaattttttc acagcagttg gtacgcccgc ttttacacgc cgggcattgc
     7141 tgacaggcaa cataagggat cacagcaact tgctgaccat ttttaagatc agcaatattt
     7201 ttacccagcc caacaatctc cccacatatt tcatggccta aaacccgtgg ataactaaaa
     7261 aatggttgat ttccacccta ggcatgaata tcggtaccgc aaatcccgac agacttaatt
     7321 tttattaatg cttcattgtc acccggaata ggtatctcgc gttgtttcca gactaattct
     7381 ttcggctgct ggcaaattaa aacattcatc gtagacatga ttcggctatc cattttttgt
     7441 tgccttagat atcaatgaaa atgaaaaatg gcagcggaac ggaaaatctg ttttgtgaaa
     7501 acacacgcat aaaaatgttt taaatcgggt tttaatggac tcacaaggag gaaaatatga
     7561 gtcgttcaca aaatttacgc cacaatgtga ttaaccaggt cattgatgat atggcgcggg
     7621 gtcatatccc ttccccactt ccatcgcaaa gtgcgctggc ggaaatgtac aacatcagcc
     7681 gcactacagt gcgtcatatt ctcagccaat tacgcgaatg cggcgtctta acgcaggttg
     7741 gaaacgacta tgttattgcg cgtaaacctg accatgatga cggtttcgcc tgtactaccg
     7801 cttcaatgag tgaacaaaac aaagtgtttg agcaggcttt tttcacgatg ataaaccagc
     7861 gtcagttacg ccctagggaa actttttctg aactgcaact ggctcgggcc gcgggtgtca
     7921 gcccggtggt tgtaagagaa tatcttttaa aattcggccg ttataatctt attcaaagcg
     7981 aaaagcgcgg ccagtggagc atgaagcaat tcgatcaatc ctacgccgag cagctgtttg
     8041 agctacgcga aatgctcgaa acacattcac ttcagcactt tctcaacctg cctgatcatg
     8101 atccccgctg gctgcaggcg aaaaccatgc tagaacgtca tcggctactg cgtgacaaca
     8161 tcggcaatag ctttcgcatg ttctcgcaac ttgacagaga ttttcactcc ttactgcttt
     8221 cagctgccga caatatcttt tttgatcaat cgcttgagat catctcagtc atctttcatt
     8281 ttcactatca atgggacgaa agcgatctta aacagcgcaa tatcattgcc gtcgatgaac
     8341 atatgaccat tctcagcgct ctgatttgcc gcagtgacct tgatgccacc cttgcgctcc
     8401 gtaaccattt gaattcagct aaacaatcaa tgattcgatc cattaatgag aacacgcgtt
     8461 atgctcatta agtacggatt aaaaacagca aactattagc gggtgcacgg ttttatcaaa
     8521 agataaatac cgggaaattc ccgtttacct atgctcacaa tcagtcgact aaaaatgttt
     8581 ttgtgagcgg tagtaaagtc ctaaaacttt aacctgccgt taatgtcaac cttgcgtaat
     8641 gcacaattac gtaaacaatt aaaattataa atacaggagt ccggcgtgga aaaagaaaat
     8701 atcaccctcg atccgcgttc ttcatttact ccatcttcgt cggcagatat tcccgtgcca
     8761 ccagatggat tagttcaacg cagtacccga attaaacgca ttcaaaccac cgccatgttg
     8821 ttattatttt ttgcggcggt aatcaattat ctcgaccgca gttcgctgtc ggtagcaaat
     8881 ttaacgattc gtgaagaatt gggattaagt gccaccgaaa tcggcgcttt gctctccgtg
     8941 ttttcactcg cttacggcat tgcgcaactt ccttgcggcc cactattgga tcgtaaaggc
     9001 ccacgcctga tgctgggact ggggatgttc ttctggtcac tgttccaggc catgtctggc
     9061 atggtgcaca gctttacgca gttcgtgttg gtgcgtatcg gtatggggat tggtgaagcg
     9121 ccgatgaacc catgcggtgt aaaagtcatt aacgactggt tcaacatcaa agagcgcgga
     9181 cgcccgatgg gcttcttcaa cgcagcttct accattggcg ttgctgtaag cccaccgatt
     9241 ctggcggcaa tgatgctggt gatgggctgg cgcgggatgt ttattaccat tggtgtactg
     9301 gggatttttc tcgccatcgg ctggtatatg ctctatcgca accgcgagca cgtagaactg
     9361 actgccgttg aacaagctta tctcaatgca ggtagcgtca atgcccgccg agatccgctc
     9421 aattttgccg aatggcgcag cctgttccgt aaccgcacaa tgtggggaat gatgctcgga
     9481 ttcagtggca tcaactacac tgcgtggctg tatctggcct ggcttcctgg ttacctgcaa
     9541 acagcctata acctggattt aaaaagcaca gggttgatgg cggctatccc tttcctgttt
     9601 ggggctgccg ggatgctggt caacggttac gttactgact ggctggtcaa agggggaatg
     9661 gctccgatta aaagccgtaa gatctgcatt attaccggga tgttctgttc tgccgccttt
     9721 acgctgatag taccacaagc gacaacatcc atgatggcgg ttctgctgat tggcatggca
     9781 ctgttctgta ttcactttgc cggaacatcc tgctggggct tgatccacgt cgcagttgct
     9841 tctcgcatga ctgcgtcggt gggcagtatc cagaactttg ccagcttcat ctgcgcctct
     9901 ttcgcgccga tcattactgg ttttattgtt gatacaaccc actcattccg tctggcacta
     9961 atcatttgcg gttgcgtcac cgcagcggga gcactggcgt acatcttcct ggttcgccag
    10021 ccgatcaacg acccgcgtaa agattaaact ccctatgccc gataacattt tgcttatcgg
    10081 gcatttttat ggcgattaaa acgtttccca gttctccccg ctatctgcca ctgccatttt
    10141 acgcggcgta gctggcgtca cggtttttac cacagccgat gtttcacgct gctgttgctg
    10201 ctgttgctga atccggaaca ctgccacagc ttcggtcagg cgactggcct gctcttccag
    10261 cgcggcggcg gcagcggcag attcttccac cagcgcggcg ttctgttgag ttacccggtc
    10321 catctcagca accgctaagc caacctgatc gataccacgg ctctgctcat cagaagcaga
    10381 agctatttcg cccataatgt ccgtcacgcg ggtcacagca ctgacaatct ccgccattgt
    10441 ttccccggcg ctttcgacca gcgtagagcc aacatccact ttccccaccg agtcttcaat
    10501 caggctttta atttcacgaa ccgcctgggc gctacgctgg gccagattac gcacttctcc
    10561 cgcaaccacc gcaaaaccac gtccttgctc acccgcacgc gctgcttcaa ccgccgcgtt
    10621 caaagccaga atattggtct ggaaggtaat gccgtcaatt acgctgataa tatcggcgat
    10681 tttctgcgaa ctggtggaga tatcgcgcat agtctgcacc acgttatcta ccactttgcc
    10741 gccgcgttgc gccgtttcag aagcacttaa tgccagatgg ctggcctggc gcgcattctc
    10801 ggcgttctgt ttcaccgttg cggtcagttg ctccatgctg gctgccgtct cttccagcga
    10861 agcggcctgt tgctcggtgc gcgaagagag atcattattg ccggtagcga tttcgctggc
    10921 accgctatag atggcattgg ccccgttgcg cacatcaccg acggtacgca tcagctctcc
    10981 ctgcatatgg cgcaaactct ctgccagttg ccccatctca ttagagccat ccacctcaat
    11041 cggtttcacc agatcgccgc ctgcaatatg acgaatgctg tcaatcaggc gattcattgg
    11101 cgctaccagc gaggctttaa taccgaacca gacggcgaag atgaccgcca gtacgacgat
    11161 catcacgccc accagaatcc acatcgcctg gctgtaggag gcattgttat cgctgacggc
    11221 gatatcatag agccgatcgt tttgctccat gtaagccaca tactgcttct cgaaaccgtc
    11281 ctgatatccc tgggtcggct gatcaaagaa ctcgttgatt ttgcctgcac ctaacagttg
    11341 gatcagctcc gccagcgcat tgtgataaat atcgtaatta cgtttgatct ctgccgctgc
    11401 ggcggtgctc tgacgcgggt cacgcggcaa cgcttcgtaa tccgcccagt ttttttccgc
    11461 ctgtttcagc gaaatactgg cactctgcat cagctcagca acggttgaac cgctaccaat
    11521 attattctga tccatcatgt agcggatacc cgcgcggttg agggtgttac gcgtctgcaa
    11581 caacgcgacc cagctgccat tcagcgtggg ttgctgctgg cgaatggttt gtaaaacagt
    11641 gaaattttct ttgtcattct ttaaggcatt aaaaaacaga ccgcctgatg tcagttgtaa
    11701 aaggccaaaa acggccaaaa ccagcagtaa gctggtcacg atcttgatac gttttaacat
    11761 ggtttctctt tcctgtggac cgatgcaaga ttttcggcct ggaaagcaaa aactttatga
    11821 aaaggtgtaa cccgccatcc atggaaaaac caggcgctgg ttatcagata ctaaaaagtt
    11881 attcattatc gcccgggatg tcttcttgtc gggaattgcc cagagcaata aattcctcta
    11941 acagatgggt taactgttgc attttctcgg cagtaaactg agcttcaatc tgccgatagg
    12001 cttcttcaat ctgtgtctga gcgcggttat acagcgcctg cccctctttg gtcagcgaga
    12061 tatagagctt ccgctgatca ttgatcggct ttaatcgcaa cactaaaccg tcgcgttcca
    12121 tccgcgtgag gatcccggtc aggcttgggc gcaaaatgca ggcgcgatac gccagatcgt
    12181 gaaaatccat tgatgggctt tccgccagaa tacgcacgat gcgccactgt tgctcagtca
    12241 aattatgccg tttaacgatt gggcgaaaat aactcatcgc cgcttctcgc gcctggagca
    12301 acgcaatggt tagtgagtcg tgcattatct ttcccctgaa aaattcaaaa tcattaatat
    12361 agaaacaatt aatcatcacg ttaccgggac tgagctgccc ccgcaattct tcattttatc
    12421 aaaattgatg atattttatt aacttactga tatttaatat tttattatct ttaaatgtaa
    12481 atagtttgtt aattagatca catttatatc acttactttt gccaaactac gcaatcggga
    12541 ataaaagtaa tcattaacat attaatgatt aagatgatcg aatccgagga gtggtaatga
    12601 aaggcactat cttcgccgta gcgttgaacc atcgcagcca acttgatgcc tggcaggaag
    12661 cgttccagca atctccctac aaagccccgc ctaaaactgc ggtctggttt attaaaccgc
    12721 gcaatacggt gattggttgc ggtgaaccga ttccctttcc acagggtgaa aaagtactga
    12781 gcggtgcgac tgttgcgctg attgtgggga aaacggcgac gaaagtacgt gaagaagatg
    12841 cggcagagta catcgccgga tatgcgctgg ataacgacgt cagcctgccg gaagagagct
    12901 tttaccgtcc ggcaatcaaa gcaaaatgtc gtgatggatt ctgccccatt ggcgaaaccg
    12961 tggctctcag caatgtcgat aatctgacta tctataccga gatcaacggg cgtcctgccg
    13021 atcactggaa taccgccgat ttacaacgta acgccgcaca gctgctgagc gccctgagcg
    13081 aatttgccac gctgaatcca ggcgatgcga ttctgctcgg cacgccacag gcgcgcgtgg
    13141 aaatacagcc aagcgatcgc gtgcgtgttc tcgcagaagg tttcccgccg ctggaaaatc
    13201 cggtagtgga cgaacgtgaa gtgaccacgc gcaagagctt cccaacgctg ccacacccgc
    13261 acggtacgct gtttgccctc ggcctgaact acgccgacca cgccagcgaa ctggaattta
    13321 agccaccgga agagccgctg gtgttcctga aagcgccgaa taccctcact ggcgacaacc
    13381 agacctccgt gcgcccgaac aatattgaat acatgcacta cgaagcggag ctggtggtag
    13441 ttattggcaa gcaggcgcgt aacgtcagcg aagccgatgc catggattat gtcgcgggct
    13501 acaccgtgtg taacgactac gccattcgcg actatctgga aaactactac cgccctaacc
    13561 tgcgggtcaa aagccgcgac ggactgacgc cgatgctttc aaccatcgtg ccgaaagagg
    13621 cgatcccgga cccgcataat ctgacccttc gcaccttcgt caacggcgag ttacgccagc
    13681 aaggcaccac cgccgatctg atcttcagcg tgcccttcct gatcgcctac ttaagcgaat
    13741 ttatgaccct gaatccgggc gacatgatcg ccaccggcac accaaaaggc ttatctgacg
    13801 tggtgcctgg cgatgaagta gtggtggaag tagaaggcgt gggccgcctg gtgaaccgaa
    13861 ttgtgagtga ggaaacagcg aaatgaaaaa agtaaatcat tggatcaacg gcaaaaatgt
    13921 tgcaggtaac gactacttcc agactaccaa tccggcaacg ggtgaagtgc tggcggatgt
    13981 ggcctctggc ggtgaagcgg agatcaatca ggcggtagcg gcagcgaaag aggcgttccc
    14041 gaaatgggct gatctgccga tgaaagagcg tgcgcgcctg atgcgccgtc tgggcgatct
    14101 gatcgaccag aacgtgcctg agatcgccgc gatggaaacc gcggacacgg gcctgccgat
    14161 ccatcagacc aaaaatgtgt tgatcccacg cgcttctcac aactttgaat ttttcgcgga
    14221 agtctgccag cagatgaacg gcaagaccta tccggttgac gacaagatgc tcaactacac
    14281 gctggtgcaa ccggtaggcg tttgtgcgct ggcgtcgccg tggaacgtgc cgtttatgac
    14341 cgccacctgg aaggtcgcgc cgtgtctggc gctgggcaat accgcggtgc tgaagatgtc
    14401 ggaactctcc ccgctgaccg ccgaccgcct gggtgagctg gcgctggaag ctggtattcc
    14461 ggcgggcgtg ctaaacgtgg tacagggcta cggcgcaacc gcaggcgatg cgctggtccg
    14521 tcatcatgac gtgcgtgccg tgtcgttcac cggcggtacc gccaccgggc gcaacatcat
    14581 gaaaaacgcc gggctgaaaa aatactccat ggagctgggc ggtaaatcac cggtgctgat
    14641 ttttgaagat gccgatattg agcgtgcgct ggacgccgcc ctgttcacca tcttctcaat
    14701 caacggcgaa cgctgcaccg ccggttcgcg catctttatt cagcagagca tctatccaga
    14761 attcgttaaa cgctttgccg aacgcgccaa ccgtctgcgc gtgggcgatc cgaacgatcc
    14821 gaatacccag gttagggcgc ttatcagtca gcaacactgg gaaaaagtct ccggctatat
    14881 ccgtctcggc attgaagaag gcgcaaccct gttggcgggc ggcccggata aaccgtccga
    14941 cctgcctgcg cacctgaaag gcggcaactt cctgcgccca acggtgctgg cggacgtaga
    15001 taaccgtatg cgcgttgccc aggaagagat tttcgggccg gtcgcctgcc tgctgccgtt
    15061 taaagacgaa gccgaaggct tacgcctggc aaacgacgtg gagtacggcc tcgcgtcgta
    15121 catctggaca caggatgtca gcaaagtgtt acgcctggcg cgtggcattg aagcaggcat
    15181 ggtgttcgtc aacacccaga acgtgcgcga cctgcgccag ccatttggcg gcgtaaaagc
    15241 ctccggcacc gggcgtgaag gcggtgagta cagcttcgaa gtgttcgcgg aaatgaagaa
    15301 cgtctgcatt tccatgggcg accatccaat tccgaaatgg ggagtctgat atgggtaagt
    15361 tagcgttagc cgccaagatc acgcacgtac cgtcgatgta tctctctgaa ctaccaggaa
    15421 aaaaccacgg ttgtcgccag ggtgcgatcg acgggcataa agagatcagc aagcgttgcc
    15481 gggaaatggg cgtcgatacc attatcgttt tcgatactca ctggctggtc aacagtgctt
    15541 atcacatcaa ctgtgcagac cattttgaag gcgtctacac cagcaacgag ctgccgcatt
    15601 ttattcgcga catgacctac aactacgagg gcaacccgga gttggggcag ctaattgccg
    15661 aggaagcctt aaagctcggc gtgcgggcaa aagcgcacaa tattcccagc ctgaaactgg
    15721 aatacggcac gctggtaccg atgcgctaca tgaatgaaga caagcacttc aaagtggtct
    15781 ccatttcggc tttctgcacg gttcacgatt ttgccgacag ccgcaagctg ggcgaagcga
    15841 ttctgaaagc gatcgaacag tacgacggca ccgtggcggt ccttgccagc ggttcgttat
    15901 cgcaccgctt tattgacgat cagcgtgcgg aagaagggat gaacagctac acccgcgagt
    15961 tcgaccgcca gatggacgag cgtgtggtga agctgtggcg cgaaggccag ttcaaagagt
    16021 tttgcaatat gctgccggag tacgccgact actgctacgg cgaaggcaat atgcacgaca
    16081 cggtgatgct gctggggatg ctcggctggg ataaatacga cggcaaggtg gagtttatta
    16141 ccgagctgtt cccaagctct ggcaccggtc aggttaacgc tgttttcccg ctgcccgcgt
    16201 aaggacgttt tatgccgcac tttatcgtgg aatgtactga aaatattcgt gaagaagccc
    16261 gcttacctga gctgtttgcc agtgttaaca ctgcactggc ggcaacgggg atttttccgc
    16321 ttgggggaat acgtagccgg gcgcactgga tcgatacctg gcaaatggct gatggcaagc
    16381 acgattacgc ctttgtgcat atgacgctaa aaatcggcag cggacgcagt ctggaaagcc
    16441 gtcaggaagt gggcgaaatg ctgtttgatc tgattaaaac gcacttcgcc tcactgatgg
    16501 agagccgtta cctagcgctt tcatttgaga ttgcagagct gcacccgacg ttgaatttca
    16561 aacagaacaa cgtacacgcg ttgtttaaat aaatttgcac cagcttccag gccggatgat
    16621 aaggcgctgc cgcatccggc aatgccacag gatatcgcta tgttcgataa acacacccac
    16681 accctgatcg cccagcgtct ggatcaggca gaaaaacagc gcgaacagat ccgcgcgatc
    16741 tcgctggatt acccggagat caccatcgaa gacgcctacg ccgtgcagcg cgaatgggtt
    16801 cgcctgaaaa tcgccgaaag tcgcacgctg aaaggccaca aaatcggtct gacctcgaaa
    16861 gcgatgcagg caagctcgca gatcagcgaa ccggattacg gtgcactgct ggacgacatg
    16921 ttcttccacg atggcagcga tatcccgacc gatcgcttta tcgtgccgcg cattgaagtg
    16981 gagctggctt ttgtgctggc aaaaccgctg cgtgggccaa actgcacgct gttcgacgtt
    17041 tacaacgcca cggactatgt gatcccggcg ctggagctga tcgacgctcg ctgccacaac
    17101 atcgatccgg aaacccagcg cccgcgcaaa gtattcgaca ccatttccga taacgccgcc
    17161 aacgccggag tgatcctcgg aggtcgtccg atcaaaccag acgaactgga tctgcgttgg
    17221 atctccgccc tgatgtatcg caatggcgtg attgaagaaa ccggcgtcgc cgctggtgtg
    17281 ctgaatcatc cggcaaacgg cgtggcctgg ctggcgaaca aactcgcacc gtatgacgta
    17341 caactggaaa ccgggcaaat cattctcggt ggttcgttca ccctcccggt tccggcgcgt
    17401 aagggcgaca ccttccacgt cgattacggc aacatgggct ccattagctg ccgctttgtt
    17461 taaggagaga acgatggaaa acagttttaa agcggcgctg aaagcaggcc gtccgcagat
    17521 tggattatgg ctggggctga gcagcagcta cagcgcggag ttactggccg gaccaggatt
    17581 cgactggtta ttgatcgacg gtgagcacgc gccgaataac gtgcaaaccg tgctcaccca
    17641 actacaggcg attgcgccct accccagcca gccagtggta cgcccatcgt ggaacgatcc
    17701 ggtgcaaatc aaacaactgc tggacgtagg cacacaaacc ttactggtgc cgatggtaca
    17761 aaacgccgac gaagcccgta aagcggtacg cgccacccgt tatccccccg ccggtattcg
    17821 cggtgtgggc agtgcgctgg ctcgcgcctc gcgctggaat cgcattcctg attacctgca
    17881 aaaagccaac gatcaaatgt gcgtgctggt gcagatcgaa acgcgtgagg caatgaagaa
    17941 cttaccgcag attctggacg tggaaggggt cgacggcgtg tttatcggcc cggcggatct
    18001 gagcgccgat atgggttatg ccggtaatcc gcagcacccg gaagtacagg ccgccattga
    18061 gcaggcgatc gtgcagatcc gcgaagcggg caaagcgccg gggatcctga tcgccaatga
    18121 gcaactggca aaacgctatc tggaactggg cgcgctgttt gtcgccgtcg gcgttgacac
    18181 caccctgctc gcccgcgccg ccgaagcgct ggcagcacgg tttggtaata gttcttctat
    18241 ttcgatggcg aagcaaaaca acaactctgt ctactaaacg tacgatgtgg agcgcatcat
    18301 gacgacctct accctgcaat ataaagataa taaaactgtt gaaattgaac atcgtgtcat
    18361 taataaatta tttcgtcgcc taatcatttt tctttttatt ctgtttgttt tttcgtttct
    18421 tgatcgcatc aatataggat ttgccggact gacaatgggc aaggatcttg gcctgacgtc
    18481 cacaatgttt ggcctcgccg caacactctt ttatgtcacg tacgtcatgt gcgggatccc
    18541 cagtaatatg atgttagcga aaattggcgc tcgtcgatgg atcgctggga tcatggtggt
    18601 ctggggaatt gcttccacct gcacaatgtt tgcaacaagc cccgaaacac tctatctact
    18661 gcgtatgctg gttggtattg ctgaggcggg ttttttacct ggtattttgg tttatctgac
    18721 ctggtggttc ccggcatatc atcgggccag agccaatgcc ttatttatga tagcgatgcc
    18781 agtcactatg atgctgggat tgatcctttc aggctacatt ctggccttgg atggactgtg
    18841 gaatctaaaa ggctggcaat ggctcttttt gctggaaggt ctgccatcgg tcattcttgg
    18901 tgtcgtcacc tggttttatc tcaacgacac accagaccag gcgacctggc ttgatgaaaa
    18961 cgaaaaacag gctttaaaaa cgatgattct ccgtgaacaa gagagtgcat ttgcccatgc
    19021 atctgttcca cgctcaacct tacgtgaagt cctgacccca gccatcatgc tatatacact
    19081 tgcttatttt tgcccgacta atactctgag tgcgattaat atctggacgc cgcaaatcct
    19141 acagagtttc aatactggca gtagcaacat catgattggt ttgctggcgg ccatccccca
    19201 attctgcaca atttttggca tgatctggtg gagtcgtcgt tccgaccgtc ttaaagaacg
    19261 caaaaagcat acgattcttc cctacctgtt tgccgctgcc ggatggttac tcgcttctgc
    19321 gacagatcac agcctcattc aactgagcag catcattatg gcctcaatgg gatcatttac
    19381 cgcgatgact atcttttgga caacaccgga tcaagttata agcttgcaat cgcgtgccgt
    19441 tgcgatggct gtcatcaatg ctgtcggcaa tgtaggttca gcagtcagtc cattacttat
    19501 cggtattttg aaagatgcaa ccggcagttt tagctcaggt ttgtggtttg tagcaggatt
    19561 gctggtactg ggcgcgttag tcatgacccg aatccctatg gggagtcggg aacactctct
    19621 ttcatctgaa cttatcgcac caaaaccgca ataaggagtc atgatgtcca gtgaagctat
    19681 ctttgaacat ggttatgaaa atattgattt ggtccgggaa tatgatgccc gctacgctgg
    19741 tgaagatgtg cattatgaaa catttgcccg gttagcgaat ttctttggac gcgatatgcg
    19801 ccctcactgg cacgatagat attttcaact ccattatctg gtcactggac gcattacttt
    19861 acaactggat gagcacttct attctgtgcg cgcaccgctt tttgtactaa caccaccatc
    19921 ggtgccacat actttcttta ctgatcctga tagcgatggg catgttctga ccgtacgtca
    19981 ggagttaatt tggcccctgg tagaaaagct ctggcctggt agtggagatg ccgcaatgct
    20041 acaaggtatc tgtctgtcac tggagggaat ggaacataca ctggcggcat taaatcatta
    20101 ttggccgata atatccctgg agtttcaaca aaataagcaa ggacgggaaa ttctattgca
    20161 atcactggcg caaagtattt ttacaatcct actgcgtgaa gcaccaccaa atgatatttc
    20221 aacatgcagc gtgcgcggcg agatgtgttt attccagaag tttaatcggc tgattgacga
    20281 aaattatcgc caacatttaa tggtccctga atatgcaaca atgctcggta tgagtgaatc
    20341 acggcttacc gagctgtgcc gacgttttgc caatcaatcg ccgaagagat tgattttcga
    20401 acgagtgagc cgggaagcca gacggcttct gctctatagc gagcaaagca tcaaccagat
    20461 agcccttgat cttggctaca aagatcccgc ctatttcgcc cgttttttca accgaatgat
    20521 gggttgctca ccaacacaat tccgtggtcg ctgaccattt atcaacctga gagactcgac
    20581 atgaaaatat ctttacgctc actatttgtg gcgtgcctga ttgcgccagt ggcattcacc
    20641 tacgcctccg aaccgcaata aatgaaaatt gtactacttg gaacgaaagg tggcccttct
    20701 ttattaaaag ctgaccgttt accgatggca acggcactta ttattggcaa taaaacctgg
    20761 ttaattgatg ctggttacgg ggccagctta caactggtaa aaaataatat tccactgcgc
    20821 cagattgata atattttact cacccattta cacagtgatc atattctgga ctatccatca
    20881 gtcttaatga atgcatgggc tagcggtctg caggacaaaa ctattcatgt attcggtccg
    20941 ccaggcaccc gagccatgac cgctgccagt tggcaggtct tcgatcggga catcacactg
    21001 cgtatggaag aagaaggaaa acccgatcct cgcaaactcg ttacagctca agatgttggt
    21061 gaaggcgtag tttataaaga tgataccgtg accgtgactg cattaaaggt ccctcattct
    21121 cctttccctg acggtgaggc ttttgcttat cgcttcgaca cacaaggcaa gcgaattgtc
    21181 ttctcaggag atacctcgta ctttccccca ctggcaactt tctcagaagg cgctgatata
    21241 cttgtgcatg aggccgtaca tgagccctcc gtcgctaagc tggcagacag tattggcaat
    21301 ggtaaaacac tggcaaaagc cattgcttct caccatacga ctattgagga tgtaggcaaa
    21361 attgccaatg aagcaaaagt gaaaaaactc atccttagcc acctggtacc aatcaccgtt
    21421 ccggatgaaa catggaagca agaagcacag aaaactattc aggtcctatc gtcgttggtc
    21481 atgaaaatat gacgattacc attccattcg ccccataaaa aattccgtcc ccgttagccc
    21541 ttgttagcgg ggaatattat gatttgcctt actgtaagtt gatcacgatc gcagtttact
    21601 atttaactcc accttgctgt cgaaaagtac caacgttcgc ctgaaacgtc tcttcccgac
    21661 aaccgcgttc aacgcttcaa ttaaataaca aaaacaacac acaaaattaa caatcgtatt
    21721 ccgattaata ctgtagaggt cgacatgaaa ccagaagatt tccgcgccag tacccaacgt
    21781 cctttcaccg gggaagagta tctgaaaagc ctgcaggatg gtcgcgagat ctatatctat
    21841 ggcgagcgag tgaaagacgt caccactcat ccggcatttc gtaatgcggc agcgtctgtt
    21901 gcccaactgt acgacgcgct gcacaaaccg gagatgcagg actctctgtg ttggaacacc
    21961 gacaccggca gcggcggcta tacccataaa ttcttccgcg tggcgaaaag tgccgacgac
    22021 ctgcgccagc aacgcgacgc catcgctgaa tggtcacgcc tgagctatgg ctggatgggc
    22081 cgtaccccag actacaaagc cgctttcggt tgcgcactgg gcgcgaatcc gggcttttac
    22141 ggtcagttcg agcagaacgc ccgtaactgg tatacccgta ttcaggaaac tggcctctac
    22201 tttaaccacg cgattgttaa cccaccgatc gtcatttgcc gaccgataaa gtgaaagacg
    22261 tttacatcaa gctggaaaaa gagactgacg ccgggattat cgtcagcggt gcgaaagtgg
    22321 ttgccaccaa ctcggcgctg actcactaca acatgattgg cttcggctcg gcacaagtga
    22381 tgggcgaaaa cccggacttc gcactgatgt tcgttgcgcc aatggatgcc gatggcgtga
    22441 aattaatctc ccgcgcctct tatgagatgg tcgcgggtgc taccggctcg ccatacgact
    22501 acccgctctc cagccgcttc gatgagaacg atgcgattct ggtgatggat aacgtactga
    22561 tcccatggga aaacgtgctg atctaccgcg attttgatcg ctgccgtcgc tggacgatgg
    22621 aaggcggttt cgcccgtatg tatccgctgc aagcctgtgt gcgcctggca gtgaaactcg
    22681 acttcattac ggcactgctg aaaaaatcac tcgaatgtac cggcaccctg gagttccgtg
    22741 gtgtgcaggc cgatctcggt gaagtggtgg cgtggcgcaa caccttctgg gcattgagtg
    22801 actcgatgtg ttcagaagcg acgccgtggg tcaacggggc ttatttaccg gatcatgccg
    22861 cactgcaaac ctatcgcgta ctggcaccaa tggcctacgc gaagatcaaa aacattatag
    22921 aacgcaacgt taccagtggc ctgatctacc tcccttccag tgcccgtgac ctgaacaatc
    22981 cgcagatcga ccagtatctg gcgaagtatg tgcgcggttc gaacggtatg gatcacgtcc
    23041 agcgcatcaa gatcctcaaa ctgatgtggg acgccattgg cagcgaattt ggtggtcgtc
    23101 acgagctgta tgaaatcaac tactccggta gccaggatga gattcgcctg caatgtctgc
    23161 gccaggcaca aagctccggc aatatggaca agatgatggc gatggttgat cgctgcctgt
    23221 cggaatacga ccagaacggc tggactgtgc cgcacctgca caacaacgac gatatcaaca
    23281 tgctggataa gctgctgaaa taacgcagca ggaggttaag atgcaattag atgaacaacg
    23341 cctgcgcttt catgacgcga tggccagcct gtcggcagcg gtaaatatta tcaccaccga
    23401 gggcgacgcc ggacaatgcg ggattacggc aacggctgtc tgctcggtca cggatacacc
    23461 accgtcgctg atggtgtgca ttaacgccaa cagtgcgatg aacccggttt ttcagggcaa
    23521 cggcaagttg tgcgtcaacg tcctcaacca tgagcaggaa ctgatggcgc gccacttcgc
    23581 gggcatgaca ggcatggcga tggaagagcg ttttagcctc tcatgctggc aaaaaggtcc
    23641 gctggcgcag ccggtgctaa aaggttcgct ggccagtctt gaaggagaga tccgcgatgt
    23701 gcaggcgatt ggcacacatc tggtgtatct ggtggagatt aaaaacatca tcctcagtgc
    23761 agaaggtcac ggacttattt actttaaacg ccgtttccat ccggtgatgc tggaaatgga
    23821 agctgcgatt taagctccag ccccttcacg cattcgtcga aggggcattt ttattgtgat
    23881 ctcaatcaca tatctacccg atgaataatt cttgcccaac cgctcaccgc cattcctgac
    23941 cgcttacatc cctaaaataa ccactcagtt atttacctta ctttacgcgc acgtatctct
    24001 ggcaacatca ctgcaggata gcggtcaatt tacctcctca aacgcaacgc aaacctagaa
    24061 cggcttcggc ctatgattaa caaattactc tatggatttt ggtttgcagg aaggcagcaa
    24121 gtgagtgaat ccccgggagc ttacaaaagt aagtgtcaga ggtgaacgaa cgcagctgcc
    24181 gcacctgtaa gccaaaagac gacgagtaaa atgccaggtt ttactatgga tacgaaaaaa
    24241 ctattcaagc acataccctg ggtgattctc ggaatcatcg gtgcattctg cctcgcggta
    24301 gttgcattac gtcgggggga gcacgtcagc gccctgtgga tcgtggtcgc ctctgtgtcg
    24361 gtgtatctgg tggcgtatcg ctactacagt ctgtacatcg cccagaaggt gatgaaactc
    24421 gaccccacgc gcgcgacgcc tgcggttatt aacaacgacg gtctgaacta cgttccgacc
    24481 aaccgttacg tgttgtttgg tcaccacttc gccgctatcg ccggtgctgg tccgctggtg
    24541 ggaccggttc tcgccgcgca gatgggctac ctgcctggca cgctgtggct gctggcgggg
    24601 gtagtgctgg ccggtgcggt acaggacttt atggtgctgt ttatctcctc tcgccgtaac
    24661 ggcgcatctc tcggtgagat gatcaaagaa gagatggggc cagtaccggg gactatcgcg
    24721 ctgtttggct gtttcttaat catgatcatc atcctcgccg ttctggcgct gattgtggtt
    24781 aaagccctgg ccgaaagtcc gtggggtgtc ttcaccgttt gctcaaccgt accgattgcg
    24841 ctgtttatgg gtatctacat gcgctttatc cgtccggggc gtgtgggtga agtctctgtt
    24901 attggtatcg tgctgctggt tgcctctatc tacttcggtg gcgtgattgc tcacgatccg
    24961 tactggggtc cggcactgac ctttaaagac accaccatta ccttcgcgct gattggctat
    25021 gcgtttgttt ccgcactgct gccggtgtgg ctgatcctcg caccgcgcga ctatctggca
    25081 accttcctga aaatcggcgt tatcgtcggc ctggcgctgg gtatcgtggt gctgaacccg
    25141 gaactgaaaa tgcctgcgat gacccagtac attgacggta ctggcccgct gtggaaaggc
    25201 gctctgttcc cgttcctgtt catcaccatc gcctgtggtg cggtatctgg cttccacgcg
    25261 ctgatctctt ccggtacgac gccgaaactg ctggctaacg aaaccgacgc gcgtttcatt
    25321 ggctacggcg caatgctgat ggagtccttc gtggcgatta tggcgctggt tgcagcgtcc
    25381 atcatcgaac cgggtcttta cttcgcgatg aacaccccgc ctgctggcct tggcatcacc
    25441 atgcctaacc tgcatgaaat gggtggcgag aacgcgccga tcatcatggc gcagctgaaa
    25501 gacgttaccg cacacgcggc agcgaccgtc agctcctggg gcttcgtgat ttcgccagag
    25561 cagatcctgc aaaccgcgaa agacattggt gagccttctg tcctgaaccg tgcaggtggt
    25621 gcgccgacgc tggcggtagg tatcgctcac gtgttccaca aagtgctgcc gatggctgac
    25681 atgggcttct ggtatcactt cggtattctg ttcgaagccc tgttcatcct gaccgcgctg
    25741 gatgcgggta cccgttctgg ccgctttatg ctgcaagacc tgctgggtaa cttcatcccg
    25801 ttcctgaaaa aaaccgactc tctggttgcc ggtatcatcg gtactgcggg ctgtgtgggt
    25861 ctgtggggct acctgctgta tcagggcgtg gtcgatccgc tgggcggcgt taagagcctg
    25921 tggccgctgt tcggtatctc caaccagatg ctggcagccg tagcgctggt actgggcacc
    25981 gttgtgctga ttaagatgaa gcgcacccaa tacatctggg taactgttgt tccggctgta
    26041 tggctgctta tctgcaccac ctgggcgctg ggtctgaaac tgttcagcac caacccgcag
    26101 atggaaggct tcttctacat ggcaagccag tacaaagaga agattgctaa cggtactgac
    26161 ctgacggcgc agcagattgc caacatgaac cacatcgttg tgaacaacta caccaacgca
    26221 ggcctgagta ttctgttcct gattgtggtg tacagcatca tcttctacgg tttcaaaacc
    26281 tggcttgcgg tgcgtaacag cgacaaacgt actgacaaag aaacaccgta cgttccaatc
    26341 ccggaaggcg gcgtgaagat ctcttcgcac cactaaccgt gtttagcccc gcttcggcgg
    26401 ggctttgttc ttatcagagt gaactatgtt tggtaactta ggacaggcaa aaaaatatct
    26461 cggccaggcg gcgaagatgt tgattggtat tccagactac gacaactacg tcgagcatat
    26521 gaagaccaac catcccgaca agccgtacat gagctatgaa gaattcttcc gcgaacgcca
    26581 gaacgcgcgc tacggcggcg atggtaaagg cggtatgcgc tgttgttaat ggagagagca
    26641 tgaacccgat tgcagttacc ctactcaccg gttttttagg cgcgggcaaa actaccctgc
    26701 tgcgccatat tcttaacgaa caacacggct acaagattgc cgtgattgaa aacgaattcg
    26761 gcgaagtctc cgttgatgac caactgattg gcgaccgcgc cacgcagatc aaaacgctga
    26821 ccaacggctg catctgctgt tcgcgctcca acgagctgga ggacgcacta ctcgacctgc
    26881 tggataatct cgacaagggc aatattcagt tcgatcgtct ggtgattgaa tgcaccggca
    26941 tggccgatcc cggcccgatt attcagacct ttttctccca tgagatttta tgccagctct
    27001 acctgctgga cggcgtgatt gcgctggtgg atgcggtaca tgccgatgag cagatgaacc
    27061 agttcaccat cgcccagtcc caggtgggct acgccgaccg tattctgctg actaaaaccg
    27121 acgtcgcagg cgaagcagag aaactgcgtg aacgcctggc gcgcatcaac gcccgcgcac
    27181 cggtctacac cgtcacccac ggcgacatcg acctgggcct gctgttcaac accaacggtt
    27241 ttatgctgga agagaacgtc gtcagcacca aaccgcgttt ccactttatc gcggataaac
    27301 aaaacgatat ttcgtcaatt gtggtggaac tggattaccc ggtagatatc agcgaagttt
    27361 cccgcgtgat ggaaaacctg ctgctggaat cggcggataa actgctgcgt tacaaaggga
    27421 tgctgtggat tgacggcgaa cctaaccgcc tgctgttcca gggcgtccag cgcctctaca
    27481 gcgccgactg ggacaggccg tggggcgatg aaaaaccgca tagcacaatg gtgtttatcg
    27541 ggattcagtt gccggaggaa gagattcggg cggcgtttgc gggattgagg aagtaagttc
    27601 gggaaagatc aactttttga gagttccacc gcaacattat tacgagtgga actctttaga
    27661 tatgtcagac caaccaaagc ttcttcaatt gcgttttcat tttttcatgt ctcattttca
    27721 gttcagccaa tccccattct gaagaattac aaacatcttt tgttaaataa aaagagcttt
    27781 tactattaag tttctcataa atagatttct ttttgataaa atcatagtgt tgtgtttcag
    27841 aatttttgga accacttatg agtgttaaat tacctatttt atgcagccag tagacatgct
    27901 cttgaggttg gaatctatta atccagtatt cattaactaa tgcttgagga agaatatgct
    27961 caattgtgat tctccccgta taggttttaa tcacgctctc atcttgttgc tccatatcta
    28021 agcgaagcaa aattgctttt attaagttca cttgattcgg acgaggctca tataaatctt
    28081 catccagagc agctataaaa cctgaattat ctgcatgttg gtttatctga tttatcacag
    28141 aatcaaaagg catatcatta tttattgcta ccagagcaga ataacaaacc atttctcttt
    28201 ggcttttaat ttgttttttt aaccacccat gcatatatac cttctcaaag gcggtaataa
    28261 attgagaaaa atcatctaaa ttaaaatctt ctgtacgagc cattcgattc atgaatgcca
    28321 taaccggagg aatccactca tctaccccta aattacttag cgatgctatt tttcgtcgta
    28381 ttgaagggtg ttcaaaatca ttctctaaaa tcttagtata
//
LOCUS       DAJTSI010000044        28404 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000044 DAJTSI010000000
VERSION     DAJTSI010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.044"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="OO327_001664"
     CDS             complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="OO327_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HCR6018238.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            321..1952
                     /gene="dcuS"
                     /locus_tag="OO327_001665"
     CDS             321..1952
                     /gene="dcuS"
                     /locus_tag="OO327_001665"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR6018239.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1949..2668
                     /gene="dcuR"
                     /locus_tag="OO327_001666"
     CDS             1949..2668
                     /gene="dcuR"
                     /locus_tag="OO327_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR6018240.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3239..4579
                     /gene="dcuB"
                     /locus_tag="OO327_001667"
     CDS             3239..4579
                     /gene="dcuB"
                     /locus_tag="OO327_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR6018241.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4657..6303
                     /gene="fumB"
                     /locus_tag="OO327_001668"
     CDS             4657..6303
                     /gene="fumB"
                     /locus_tag="OO327_001668"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6018242.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAENNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6426..7055
                     /locus_tag="OO327_001669"
     CDS             6426..7055
                     /locus_tag="OO327_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR6018243.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7194..8615)
                     /gene="melB"
                     /locus_tag="OO327_001670"
     CDS             complement(7194..8615)
                     /gene="melB"
                     /locus_tag="OO327_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCR6018244.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8718..10073)
                     /gene="melA"
                     /locus_tag="OO327_001671"
     CDS             complement(8718..10073)
                     /gene="melA"
                     /locus_tag="OO327_001671"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR6018245.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            10356..11264
                     /gene="melR"
                     /locus_tag="OO327_001672"
     CDS             10356..11264
                     /gene="melR"
                     /locus_tag="OO327_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR6018246.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11463..13730
                     /gene="adiA"
                     /locus_tag="OO327_001673"
     CDS             11463..13730
                     /gene="adiA"
                     /locus_tag="OO327_001673"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR6018247.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            14055..14816
                     /gene="adiY"
                     /locus_tag="OO327_001674"
     CDS             14055..14816
                     /gene="adiY"
                     /locus_tag="OO327_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR6018248.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            14953..16290
                     /gene="adiC"
                     /locus_tag="OO327_001675"
     CDS             14953..16290
                     /gene="adiC"
                     /locus_tag="OO327_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR6018249.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            16394..18037
                     /gene="eptA"
                     /locus_tag="OO327_001676"
     CDS             16394..18037
                     /gene="eptA"
                     /locus_tag="OO327_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR6018250.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAELIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            18034..18702
                     /gene="basR"
                     /locus_tag="OO327_001677"
     CDS             18034..18702
                     /gene="basR"
                     /locus_tag="OO327_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR6018251.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            18703..19803
                     /gene="pmrB"
                     /locus_tag="OO327_001678"
     CDS             18703..19803
                     /gene="pmrB"
                     /locus_tag="OO327_001678"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR6018252.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(19779..19868)
                     /gene="pmrR"
                     /locus_tag="OO327_001679"
     CDS             complement(19779..19868)
                     /gene="pmrR"
                     /locus_tag="OO327_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR6018253.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(19980..21482)
                     /gene="proP"
                     /locus_tag="OO327_001680"
     CDS             complement(19980..21482)
                     /gene="proP"
                     /locus_tag="OO327_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR6018254.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(21746..22624)
                     /locus_tag="OO327_001681"
     CDS             complement(21746..22624)
                     /locus_tag="OO327_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR6018255.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(22621..24849)
                     /gene="crfC"
                     /locus_tag="OO327_001682"
     CDS             complement(22621..24849)
                     /gene="crfC"
                     /locus_tag="OO327_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR6018256.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            25252..25587
                     /locus_tag="OO327_001683"
     CDS             25252..25587
                     /locus_tag="OO327_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR6018257.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            25745..26188
                     /gene="yjdN"
                     /locus_tag="OO327_001684"
     CDS             25745..26188
                     /gene="yjdN"
                     /locus_tag="OO327_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCR6018258.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            26321..27109
                     /gene="phnC"
                     /locus_tag="OO327_001685"
     CDS             26321..27109
                     /gene="phnC"
                     /locus_tag="OO327_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR6018259.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            27134..28150
                     /gene="phnD"
                     /locus_tag="OO327_001686"
     CDS             27134..28150
                     /gene="phnD"
                     /locus_tag="OO327_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6018260.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            28256..>28404
                     /locus_tag="OO327_001687"
     CDS             28256..>28404
                     /locus_tag="OO327_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HCR6018261.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMAT"
BASE COUNT         7296 a         7418 c         6935 g         6755 t
ORIGIN      
        1 gactttcgtt tgagattaaa taacttgccg ttgccacgta cgcaattgcc agaatgctga
       61 catatcgcag tgttgaaata gacatcgatc ttttcgccgg tataacagcg ataacccccg
      121 tccagtagcg cctgatccat gaccttccct ctctcatcag tatgattatc gctataagca
      181 tagccccgga atcttcatac agcactggca gtttccgttg ccagagtctc ttcccctata
      241 tagccaatca tttttactcc ctttgaatta cccgcctcat cagagataat gcttaagaaa
      301 tttgtcacac aaggaagctg atgagacatt cattgcccta ccacatatta cgcaaacgtc
      361 cgatgaaatt gagtaccact gtgatcttaa tggtcagcgc ggtactgttc tcggtgctat
      421 tggtggtgca tctgatttac ttctcgcaaa tcagtgatat gacgcgagac gggctggcca
      481 acaaggcact ggcagtggcg cgtaccctcg ccgactcgcc ggaaatccgt cagggcttgc
      541 agaaaaaacc gcaggagagt ggcatccagg ccatcgcgga agccgtgcgc aaacgcaacg
      601 atctgctgtt tattgtcgtt accgatatgc aaagtcttcg ctactcacat cctgaagccc
      661 agcgtattgg tcagccattt aaaggtgatg acatccttaa tgcgctgaat ggcgaagaaa
      721 atgtcgctat caatcgcggt tttctggcgc aggctttacg cgtatttacc cccatctacg
      781 atgaaaatca taaacaaatt ggcgtggtgg cgatcggcct tgagttaagc cgcgtgaccc
      841 aacagatcaa tgacagtcgc tggagcatta tctggtcggt attatttggc atgctggtcg
      901 gactgattgg cacctgcatt ctggttaagg tactgaaaaa aatccttttc ggcctggaac
      961 cctacgaaat ctccacgctg tttgagcaac gccaggccat gttgcagtct atcaaagaag
     1021 gcgtcgttgc cgtggacgat cgcggcgagg tcacgctgat caacgatgcc gcacaagaat
     1081 tgctgaatta ccgtaagtcg caggacgatg agaaactgtc gacgctcagc cactcatggt
     1141 cacaggtggt agatgtctcg gaagtgttac gcgacggtac gccgcgccgc gacgaagaga
     1201 ttacgattaa agaccggcta ttactgatca acaccgttcc ggtgcgcagt aatggcgtta
     1261 tcatcggtgc catttcaacc ttcagggaca aaactgaagt tcgtaaactg atgcagcgac
     1321 tcgacggtct ggtcaactat gctgacgcac ttcgtgaacg atcccacgaa tttatgaata
     1381 aattgcatgt gattctcgga ttattgcatc tgaagagtta taagcagttg gaagattaca
     1441 ttctcaaaac agccaataac tatcaggaag agattggctc tctgctgggt aagatcaaat
     1501 ctccggttat cgctggtttt ttaatcagca agattaaccg cgcgaccgat ttaggccata
     1561 cgctgatttt aaacagtgaa agccagctgc cagacagcgg cagtgaggac caggtcgcga
     1621 cgctgattac cacattggga aatctgatag aaaacgcgct ggaagcatta gggccggaac
     1681 ccggtggcga aattagcgta acattgcact accgtcacgg ctggctgcac tgcgaagtta
     1741 acgatgatgg accggggatc gcacccgaca aaatcgatca catttttgac aaaggtgtct
     1801 cgacaaaagg aagcgagcga ggcgtcggtt tagcacttgt caaacaacag gtagaaaatc
     1861 tcggcggcag catcgccgtg gaatcggaac cggggatttt cacacaattt tttgtccaga
     1921 taccctggga cggggagagg tcgaacagat gatcaatgta ttaattatcg atgacgacgc
     1981 aatggtcgcg gagctgaatc gccgatacgt agcacaaatc ccaggctttc aatgctgtgg
     2041 aacagcctcg acgctggaga aagccaaaga gattatcttc aatagcgatg cgcctatcga
     2101 cctgatattg ctcgatatct atatgcaaaa agagaacggg ctcgatttac tgcctgtcct
     2161 gcataacgcg cgttgcaaaa gtgatgtgat tgtcatctcc tccgcagccg atgcggcaac
     2221 cattaaagat tcgctgcatt acggtgtcgt ggattacctg atcaaaccct tccaggcttc
     2281 ccgctttgaa gaggcgctca ccggctggcg gcaaaagaaa atggcgctgg aaaaacatca
     2341 gtattatgac caggccgagc ttgatcagct aatccacggc agcagctcca acgaacagga
     2401 tcctcgtcgc ttgccaaaag gcttaacgcc gcagacgtta cgcacgctgt gccagtggat
     2461 tgacgcgcat caggactatg agttttcaac cgacgagctg gctaacgagg ttaacatctc
     2521 gcgtgtttcc tgccgaaaat acctcatctg gctggtcaac tgccacatct tgttcaccag
     2581 tatccattat ggcgtcacgg ggcgtccggt ttaccgttac cgtattcagg cagaacacta
     2641 ctcactgctg aaacaatatt gccaataacg gatcgctggt tatctgtaag taataattat
     2701 taccgtaatg caaaataccc ctggcacaag ttacatcgtt aatcagtaaa acttgtgcca
     2761 gatcaaatat aattatccct ccatcatcgt taaaaattaa tatctcttca gatgaacggt
     2821 gtttttaatt tcaaaacgat aacaaaagtt aattaactat tatgtcaccc gcattatgtg
     2881 tatttttacc cacaaatggg tagatcagat taatctataa acctaatgat atctgccctg
     2941 agaacaaaaa atagaccgat aaatatcaat aagataacag caaacaaaac attaacatct
     3001 gcgcagtaca aactataaac ccatcgccag agagtttttc tctctgaaaa agccgcttat
     3061 cacagtgcat aaatttgccg ctgctttaat cagccaatat tcactgtgag gtatttgcta
     3121 aagccggtaa cgaccaaacg gatatttagt caggctctga aaacagttca tacaaaacag
     3181 aacgtgactg tgatctattc agcaaaaatt taaataggat tatcgcgagg gttcacacat
     3241 gttatttact atccaactta tcataatact aatatgtctg ttttatggtg ccagaaaggg
     3301 tggtatcgcg ctgggtttat taggcggtat cggtctggtc attctggtct tcgtcttcca
     3361 ccttcagcca ggtaaaccac cagttgatgt catgctggtt atcattgcgg tggtggcggc
     3421 atcggcgacc ttgcaagctt cgggcggtct tgatgtcatg ctgcaaattg ccgagaagct
     3481 gctgcgccgc aacccgaaat atgtctcaat tgtcgcgccg tttgtgacct gtacactgac
     3541 cattctttgc ggtacgggtc atgtggttta caccattctg ccgatcatct acgacgtcgc
     3601 cattaagaac aacatccgtc cggaacgtcc gatggcggca agttctatcg gtgcacagat
     3661 ggggattatc gccagtccgg tgtcggttgc ggtcgtgtct ctggttgcga tgctgggtaa
     3721 tgtcaccttt gatggtcgcc atcttgagtt cctcgatctg ctggcaatca ccattccatc
     3781 gacgttaatc ggtatcctgg cgatcggtat cttcagctgg ttccgcggta aagatctgga
     3841 taaagacgaa gagttccaga aattcatctc cgtaccggaa aaccgtgagt atgtttacgg
     3901 tgataccgcg acgctgctgg ataaaaaact gccgaaaagc aactggctgg caatgtggat
     3961 tttcctcggg gcaatcgctg tagtcgccct tcttggtgct gattcggacc tgcgtccatc
     4021 cttcggcggc aaaccgctgt cgatggtact ggttattcag atgtttatgc tgctgaccgg
     4081 ggcgctgatt attatcctga ccaaaaccaa tcccgcgtct atctcaaaaa acgaagtctt
     4141 ccgttccggt atgatcgcca tcgtggcggt gtacggtatc gcatggatgg cagaaaccat
     4201 gttcggtgcg catatgtctg aaattcaggg cgtactgggt gaaatggtga aagagtatcc
     4261 gtgggcgtat gccattgttc tgctgctggt ttccaagttt gtaaactctc aggctgcggc
     4321 gctggcggcg attgttccgg tcgcgctggc gatcggcgtt gatccggcat acatcgtggc
     4381 ttcagcaccg gcttgctacg gttattacat cctgccgact tatccgagcg atctggcagc
     4441 gattcagttt gaccgttccg gcaccaccca catcggtcgc ttcgtcatca accacagttt
     4501 tattctgccg gggttgattg gtgtgagcgt atcgtgcgtc ttcggctgga tcttcgccgc
     4561 gatgtacggg ttcttataaa tgcactttgc gtgccgcccg tgactacgcg gcacgccatt
     4621 ttcgaataac aaatacagag ttacaggctg gaagctatgt caaacaaacc ctttatctac
     4681 caggcacctt tcccgatggg gaaagacaat accgaatact atctactcac ttccgattac
     4741 gttagcgttg ccgacttcga cggcgaaacc atcctgaaag tggaaccaga agccctgacc
     4801 ctgctggcgc agcaagcctt tcacgacgct tctttcatgc tacgtccggc acaccagaaa
     4861 caggttgcgg ctattcttca cgatccagaa gccagcgaaa acgacaagta cgtggcactg
     4921 caattcttaa gaaactccga aatcgccgcc aaaggcgtgc tgccgacctg ccaggatacc
     4981 ggcaccgcga tcatcgtcgg taaaaaaggc cagcgcgtgt ggaccggtgg cggtgatgaa
     5041 gaagcgctgt cgaaaggcgt ctataacacc tatatcgaag ataacctgcg ctactcgcaa
     5101 aacgcggcgc tggacatgta caaagaggtc aacaccggca ctaacctgcc tgcgcaaatc
     5161 gacctgtacg cggtagatgg cgatgagtac aaattccttt gcgttgcgaa aggcggcggc
     5221 tctgccaaca aaacgtatct ctaccaggaa accaaagccc tgctgactcc cggcaaactg
     5281 aaaaacttcc tcgtcgagaa aatgcgtacc ctcggtactg cagcctgccc gccgtaccat
     5341 atcgcgtttg tgattggcgg tacgtctgcg gaaaacaacc tgaaaaccgt caaattagca
     5401 agcgctcact attacgatga actgccgacg gaagggaacg aacatggtca ggcgttccgc
     5461 gatgtccagc tggaacagga actgctggaa gaggcccaga aactcggtct tggcgcgcag
     5521 tttggcggta aatacttcgc gcacgacatt cgcgttatcc gtctgccacg tcacggcgca
     5581 tcctgcccgg taggcatggg cgtctcctgc tccgctgacc gtaacattaa agcgaaaatc
     5641 aaccgcgaag gtatctggat cgaaaaactg gaacacaatc taggtcagta cattccacaa
     5701 gaactgcgtc aggccggtga aggcgaagcg gtgaaggtcg accttaaccg cccgatgaaa
     5761 gagatcctcg cccagctttc gcaatatccg gtttccactc gtttgtcgct cactggcacc
     5821 attattgtgg gtcgagatat tgcacacgcc aagctgaaag agctgattga cgccggtaaa
     5881 gaacttccgc agtacatcaa agatcacccg atctactacg cgggtccggc gaaaacccct
     5941 gccggttatc catcaggttc acttggccca accaccgcag gtcgtatgga ctcctatgtg
     6001 gatctgctgc aatcccacgg cggcagcatg atcatgctgg cgaaaggcaa ccgcagccag
     6061 caggttaccg acgcgtgtca taaacacggc ggcttctacc tcggtagcat cggcggtccg
     6121 gcggcggtac tggcgcagca gagcatcaag catctggaat gcgtcgctta tccggagctg
     6181 ggtatggaag ctatctggaa aatcgaagta gaagatttcc cggcgtttat cctggtcgat
     6241 gacaaaggta acgacttctt ccagcaaatc gtcaacaaac agtgcgcgaa ctgcactaag
     6301 taacctcttc ggcccagcgc ctggcagcat gctgccaggt gatccccctg ggccacctct
     6361 tttgcgattg taatttcatg cttgctggtg aatagtcagt attttccctg acttgcgaac
     6421 tcaccatgac ccgcacactc aagccgttaa ttcttaacac tggcgcactg gcgctaacgt
     6481 taatcctgat ttataccggc atttcggccc atgacaaact cacctggctg ctggaagtga
     6541 caccggtgat tatcgtcgtg ccgctactac ttgccaccgc cagacgttac ccgttaacgc
     6601 tgctgctcta tacgctcatc ttctttcacg ccatcatcct gatggtaggc gggcagtaca
     6661 cctacgcgaa agtccccgtt ggttttgagg tgcaggaatg gctggggttg agtcgtaatc
     6721 cgtatgacaa gctggggcac tttttccagg ggctggtgcc tgcactggtg gcacgagaaa
     6781 ttctcgttcg cgggatgtac gtgcgcggac gtaaaatggt ggcgtttctg gtctgttgcg
     6841 tggcgctggc gataagcgcg atgtatgaat taatcgagtg gtgggcggca ctggcgatgg
     6901 gccagggagc ggatgatttt ctcggtaccc agggcgacca gtgggacacg caatccgata
     6961 tgttctgcgc gctgcttggc gcattaacga cagtgatatt gctcgctcgc tttcattgcc
     7021 gccagctacg gcgctatggc ttgataactg gataacaacg tgcactttgt tgatgccgga
     7081 tgcggcgtga attccttatc cggtctacaa aatttgcaaa tccaaagcat tacattaaga
     7141 cgtggcctga taaacatatc gcatcaggcc attttcgtta gctattgtcc acgtcaggct
     7201 ttcacatcac tcactgcacc gaccggaata tcagcatgaa caggctcggg cggtactttg
     7261 cgatatttat ccagcagatg aatctggatc ctgcgcagcg tgtcgccatt gaggcgatag
     7321 aaacggaagt agagaatcag cgttaccatg aaaaacagag tcggtagagc aatcataata
     7381 aactgcatac ctaatagggc ttgcgtagac tgttcaacat tcggtacata gccaatcatc
     7441 cctaacacaa ccgcaatgaa aaaagccgca aaggctgaac cgcccttcac caccatagtc
     7501 tgcacggagt aagcgatgct ttcacagcgt acgtgcagtt tatattcacc gtaatcaacg
     7561 gtatctgcca ccatgatgac ctgtaatacc cagaaaagcg ccgttcccac attcagcaaa
     7621 atacccgcaa tcacaatgag gacgacgttg tgatagctca ttaatgccat taacaggaga
     7681 acaccacagc ttaacaccgg aagaatagat gctccggccc ataaaatgcg tcgggataat
     7741 gatttaacta agcgtgggaa gaatactaac gtcaccaggt tagcagctcc cgcatacgac
     7801 agataatagg ggaacaaatc cgcatcaccg ataacatatg agaaataata gatagcaaat
     7861 ccggtaataa tgttgctggc tacattataa gcaagagcca tacccaagag gcatgaaagc
     7921 tgatcgtttt tataaataag cgcaacgatg gctttaagtg tcagatggct tccttcagca
     7981 gacgattgat tgtctgacga aaagacttca tgcacattgc gcagagtgat gatggttgaa
     8041 acaataaaaa aggcgatcaa taccagagtg aacatctgaa agccaaatcc ccgatcgcca
     8101 ccgccgacat aattaacaaa tggtagcgtc acacctgccg taacaaagcc tgccagactg
     8161 gcaaaaaaac gcggataagg aaccagttgt tcgcgttcac gtttatcgag cgtgatggtt
     8221 ggaaccagcg accagaaggg aatatccata atggtgtaag tcatgcccca gaggatgtag
     8281 gtcacgcaaa caaagacaat ctgagtagta ccttcaaaca gatgcgcact aaagaggaga
     8341 aataagatta cagagtttgc caacgtaccg atcaggatcc agggtttgaa cttaccccat
     8401 cgcgatcgcg tagcatttac aatccatccc ataatcggat cgttaatagc atcccagatc
     8461 ctcgccacca gaaacaaagt accgaccaaa cccacagaca gcccgacgac atcggtgtag
     8521 taatacatga ggtacatata cacaatgccg atcgcaaaat ccttcccgaa cgctccaaat
     8581 ccataactga gttttgtagt cattgaaatg ctcatagggt atcgggtcgc tgttaccaac
     8641 gcccgccttc tgttattggt atcgtcagag gtgtgcgccg gaataaatcc cccggcgcag
     8701 tagcgtttag tcgcgtttta acggtgcaac cagcttggca gccagtcgcc gtgggcggca
     8761 atcaggtcgt caacaagagc atatatttcg tcaatgccca gcacggcggc agtatgcggg
     8821 tccatcatcg cggcgtggta aacacggtcg cgattttccg taagaatagc ttcggtcagc
     8881 agcgtctgta cgttgatgtt ggtttgcatc agggcggcca gatgcgaagg tagcgtaccg
     8941 actttggtcg gctgaatgcc attagcatca accagacagg ctacttccac gcaacatcct
     9001 tgtggcaggt tatcaatcaa accatcgtta cggacgttgc cgtaaatcac actcggctcg
     9061 ccagtccaga tagcgttcat gattgtgctg gcatattccc gtgacggttt aatatcaatc
     9121 cgggaggcgt ttttatactc ctccagctct ttatgccagt tcgccagctg ctcgacgcag
     9181 cgtttcgggt actcatccag cggtacttta taacgctcaa tcaaatcctc acgacctagc
     9241 ttaataaacc acggcgtata ttcggcaaaa tgttctgatg attccgtgac gaagtagccc
     9301 agctttttga acatttcgta gcgcacaata ttctggcagc gagtattacc atgaatattt
     9361 ggcttcggtg cctgccctgc ttcataagcc gccagcagtt ccgggtagag attcacgtaa
     9421 ctgccgtcgg cggttttgcg ctccagctcc aggtaaaacg ccatatggtt gatacctgcg
     9481 caacggtaac gcagcgtagc cgggtcgata ttgaggtcac gcgccagctc ttccgccgtt
     9541 ccctgcaccg aatggcacag cccgacctgt ttgatatacg gatagcgggc atacatcgcc
     9601 caggtattca tcgccattgg gttaacatag ttgagcatgg tggcatcggg gcagacttcc
     9661 gtcatgttct cgcaaatttg ccacagatgc ggaatggtac gtagcgcgcg cataataccg
     9721 cccggcccca acgtatcggc aatggtttgt tccagaccat gccgcttaca gacctcgaaa
     9781 tcagtcaccg tgcaaggttc ataaccgcca atctgaaatg ccaccacgac aaaatcggca
     9841 tcctctaagg cttctttctg ttgggtgtgg caggtgattt tgccgctggc ccctgctgaa
     9901 tccatcagct tacgcaccac aatatgcgac tcttccaggc gggtggggtc aatgtccatc
     9961 agggcaatat gcgccgtttt cagcgcctcg cgatggaaca catcaccaag aatattttta
    10021 acgaaaatcg tcgaaccagc gccgataaat gtaattttgg gtgcagacat catgcagatc
    10081 tcctggcttg cttgaataac ttcatcatgg caggcgaatc tgcagaaaac ctccggcttc
    10141 caggttgagc attcccataa actcagatcc tgcgtgaagc agcagtaaat ctgagtttat
    10201 gggaataaat tacctgaaaa gtagagtttt cggcctcatg atgctcggac agtcccctcg
    10261 cccctctggg aagagggtga aaaccgtgct cccactcgca gtcatcctcc ctcactcctg
    10321 ccataattct gatattctag gaaagagagc catccatgaa tacagatacg tttatgtgca
    10381 gcagcgacga aaaacagacc cgcagcccgc tgtcgctgta ttcggaatac cagcggatgg
    10441 agattgagtt tcgcgcaccg catatcatgc ccaccagcca ctggcatggt caggtcgaag
    10501 tgaatgtgcc tttcgatggc gatgtggaat acctgatcaa caatgaaaaa gtgaatatca
    10561 atcagggtca tatcacgctg ttctgggcct gtacaccgca ccaactaaca gataccggaa
    10621 cctgtcagag catggcgatt tttaatctgc cgatgcatct gtttctctcc tggccgctgg
    10681 ataaagacct gattaaccac gtcactcacg gcatggtgat caaatcactt gccacacagc
    10741 aacttagccc gtttgaagta cgccgctggc agcaggaatt aaacagtccg aatgagcaaa
    10801 ttcgccagct cgccattgat gaaattggcc tgatgctcaa gcgatttagc ctctctagct
    10861 gggaaccgat tctggtcaat aaaacctcgc gcacacacaa aaacagcgtc tcgcgccatg
    10921 cgcaatttta tgttagccag atgctgggct ttattgccga aaactatgat caggcgctga
    10981 ccatcaacga tgtggctgag cacgtcaaac ttaacgccaa ctatgcaatg gggatattcc
    11041 agcgggtcat gcaattgacg atgaaacagt acattaccgc gatgcgcatc aaccacgttc
    11101 gcgcgttact gagcgatacc gataaaagta ttctcgatat tgccctgacg gcaggctttc
    11161 gttcaagtag tcgtttttac agcacgttcg gcaaatatgt cggcatgtcg ccgcaacaat
    11221 accgcaaact tagccaacag cgccgccaga cgtttcccgg ctaaagacga tatcagtatc
    11281 agccaaaaaa atagtttagc cgtcgataat aagcagtgaa aataatttaa aataatcaca
    11341 taacgtattg tttataaaac taaatatttc aaaaatgttt gtttttcacg cgctttacag
    11401 cccgaaaagg ccggaagata cttgcccgca acgaagattc cttcataacc gggtaagcaa
    11461 tgatgaaagt attaattgtt gaaagcgagt ttctccatca agacacctgg gtcggtaacg
    11521 ccgttgagcg tctggcagat gctttaagcc agcaaaatgt taccgtgatt aaatccacct
    11581 cctttgatga tggttttgcc attctctctt caaacgaagc cattgactgc ctgatgttca
    11641 gctatcaaat ggagcatccg gacgaacatc aaaacgtcag acaattgatc ggtaagcttc
    11701 atgagcgcca acaaaacgtg ccggtcttcc tgttgggcga tcgggaaaaa gccctcgccg
    11761 caatggaccg ggatctgctg gagcttgtcg atgaattcgc ctggattctg gaagataccg
    11821 ccgactttat cgccggacgc gccgttgccg cgatgacccg ctaccgccag cagctgttgc
    11881 cgccactgtt cagcgcgctg atgaaatata gtgacatcca tgaatattcc tgggcagcgc
    11941 caggccacca gggcggcgtt ggttttacca aaacacccgc cggacgtttc taccatgact
    12001 actatggtga aaatctgttc cgtaccgaca tgggcatcga acgaacttcc ctcggttctt
    12061 tgcttgacca tactggcgca tttggcgaaa gcgaaaaata tgccgcacgc gtatttggtg
    12121 ccgatcgctc ctggtcggta gtcgtcggta cttccggctc taaccgcacc atcatgcagg
    12181 cttgcatgac cgataacgat gtcgtggtcg ttgaccgtaa ctgccataaa tccatcgaac
    12241 aaggtttgat gctgacaggc gcgaaaccgg tctatatggt gccaagccgc aaccgctacg
    12301 gcattatcgg gccaatctat ccgcaggaaa tgcaacctga aaccttgcaa aagaaaatca
    12361 gtgaaagccc gctgaccaaa gacaaagccg ggcaaaaacc gtcttactgc gtggtaacca
    12421 actgcaccta tgacggcgtg tgttataacg ctaaagaagc gcaggatctg ctggaaaaaa
    12481 cctccgatcg tctgcacttt gacgaagcct ggtacggcta tgcacgtttc aacccgatct
    12541 atgccgatca ctatgccatg cgcggcgaac ctggcgatca caacggtcct accgttttcg
    12601 ccacccactc catccacaaa ctgctgaatg cgctgtcaca ggcttcttat attcatgtac
    12661 gtgaaggtcg tggggcgatt aacttctccc gcttcaacca ggcctacatg atgcatgcca
    12721 ccacctcccc gctgtatgcc atctgcgcat ccaacgacgt ggcggtgtcg atgatggacg
    12781 gcaacagcgg cctgtcactg acacaggaag tgattgacga agcggttgat ttccgtcagg
    12841 cgatggcgcg gctatataaa gagttcaccg ctgacggtag ctggttcttc aaaccgtgga
    12901 acaaagaagt cgtcaccgac ccacaaaccg gcaaaaccta tgactttgct gacgcaccaa
    12961 ccaaactgct gaccaccgtt caggactgct gggtaatgca tccgggcgaa agctggcacg
    13021 gcttcaaaga tattccggat aactggagta tgctcgaccc aattaaagtc agcatccttg
    13081 ctccgggaat gggtgaagat ggtgaactgg aagaaaccgg tgttccggcg gcgctggtca
    13141 ctgcctggct tggtcgccac ggcattgtgc ctacccgcac cactgacttc caaattatgt
    13201 tcctgttctc tatgggcgta acccgtggga aatgggggac tctggttaac accctttgct
    13261 ccttcaaaca ccactatgac gccaacacac cgctggcgca ggtgatgccg gaacttgttg
    13321 aacaatatcc tgacacttac gcgaacatgg ggattcacga tctgggtgac accatgtttg
    13381 cctggctgaa agaaaacaac cctggcgcac ggttgaacga agcctattcc ggcctgccgg
    13441 tggcggaaat caccccgcgt gaagcgtaca acgcgattgt cgacaacaat gtcgaactgg
    13501 tatccattga aaatctgcca ggacgcatcg cggcaaactc agttatcccg tatccgccag
    13561 gaatcccgat gctgctgtct ggtgaaaact tcggcgataa aaacagtccg caagtaagtt
    13621 atttacgctc gctgcaatcc tgggaccacc atttccctag atttgaacac gaaactgaag
    13681 ggactgaaat tattgacggt atttaccacg ttatgtgcgt gaaagcgtaa ccactattcc
    13741 gctgaaggcg taattgttta aataacatta cgccgcctgg ccttaggcct tttgagtatg
    13801 gcaacgtttt cataaaaatt gctgcaaaca aaaatgtcat actttttgcg cggccccacc
    13861 ccacgctttt ttttgcctgt tatttatcct gtaaaaaaat atgtacatga gaaattacta
    13921 taaaaatttg taatattagt aaaactcgtt atttttatgc atgtttatat tcatcatgca
    13981 attatataac catttcccgg tatcgctttg ctttagcgag aactggtctt ttattcgcac
    14041 tcaggagtac atgtatgagg atttgcagcg accaaccttg tattgtttta ttgactgaaa
    14101 aagatgtctg gataagggtg aatgggaaag aacctattag ccttaaagct aaccatatgg
    14161 cgttattaaa ttgtgaaaat aatattatcg acgtctcctc tcttaacaac actttggttg
    14221 ctcatattag tcacgacatc atcaaagatt acctccggtt tctgaataaa gatctctcgc
    14281 aaataccagt atggcaacgt agcgctacgc ccatactcac cctgccatgc ctgacgccag
    14341 acgtctttcg cgttgccgcg caacacagca tgatgcccgc agaaactgag tcagaaaagg
    14401 aacgaacacg cgcattatta ttcacggtgc tatcccgttt tctcgacagt aaaaaattcc
    14461 tttcattaat gatgtatatg ttacgtaatt gtgtaagtga cagcgtttat caaattattg
    14521 aaagcgatat tcacaaagac tggaatctta gtatggtagc cagttgttta tgtcttagcc
    14581 caagtctgtt aaagaaaaag ttgaaaagcg aaaacaccag ttatagccaa ataatcacca
    14641 cctgccgcat gcgttatgcc gtaaatgaat taatgatgga cggtaaaaat atctctcagg
    14701 tatcacagtc ctgcggctac aacagtacgt cgtactttat ttctgtcttt aaagacttct
    14761 acggtatgac gccgctgcat tatgttagtc agcacagaga acgcactgtc gcctgatttt
    14821 taaccttaac gaagagctat attaataacg gcatcagcga taacccggtc gataataatt
    14881 caactatcga atgcaggcgt atgatatgac gtaatttatt gtcacgaagc tcgccttcgc
    14941 aggagtttaa ttatgtcttc ggatgctgat gctcacaaag tgggcttaat ccccgtcacc
    15001 ctgatggtgt cggggaatat tatggggtca ggtgtttttc tgttacctgc aaacctggcc
    15061 tccactggcg ggattgccat ttatggatgg ttggtgacga ttatcggtgc gctggggctc
    15121 tcgatggtat acgccaaaat gtcgttcctc gacccaagtc ctggtggttc ttacgcttac
    15181 gcccgccgct gctttggccc gtttctcggt tatcaaacca acgtcctcta ctggctggcc
    15241 tgctggatcg gcaatatcgc catggtggtc attggcgtag gatatttaag ttacttcttc
    15301 ccgattctga aagatccatt ggtattaacc atcacctgcg tcgtggtgct gtggatcttc
    15361 gtcctgctga acattgtcgg tccgaaaatg atcacccgcg tgcaggccgt tgccactgtg
    15421 ctggcgctga tccccatcgt cgggattgcc gtatttggct ggttctggtt ccgtggtgaa
    15481 acctatatgg cggcatggaa cgtcagcggc ctgggcacct tcggtgcgat tcaaagtacc
    15541 cttaacgtta cgctgtggtc gttcatcggt gtggaaagtg cctccgttgc cgcaggtgtg
    15601 gtgaaaaacc cgaaacgcaa tgtccctatc gccaccattg gtggggtatt gattgccgcc
    15661 gtttgctatg tgctctccac caccgcgatt atggggatga tccctaatgc cgcactgcgc
    15721 gtttctgcct cgccattcgg tgatgccgca cggatggcgt tgggtgacac cgctggggcc
    15781 attgtttcct tctgcgcagc tgcgggttgc ttaggttcac tgggcggctg gacgttgctg
    15841 gcgggtcaaa cggcgaaagc cgctgccgat gacggactgt tcccaccgat ttttgcccgt
    15901 gtaaataaag cgggtacgcc agtggcgggg ctgattatcg tcggtatttt gatgaccatc
    15961 ttccagctca gcagcatttc accaaacgcg accaaagagt tcggtctggt ttcttccgtc
    16021 tcggtcatct ttacactggt gccatatctt tacacctgtg cggcgttact gctgctcggc
    16081 cacggtcact ttggtaaagc acgcccggca tatctggctg ttactaccat tgccttcctc
    16141 tactgcattt gggccgtggt ggggtccggg gcgaaagagg ttatgtggtc atttgtcacc
    16201 ctgatggtca tcaccgccat gtatgcgctg aattacaacc ggctacataa aaacccgtat
    16261 cccttagatg caccaataag caaagattaa ttccccttaa tccagcaaac ataaaagcca
    16321 accttaagaa cttaaggttg gcttaatttt gctttgcgag catatgcgca ctttgttcga
    16381 tggaaacacc gtgatgttga agcgcctact aaaaagaccc tctttgaatt tactcgcctg
    16441 gctattgttg gccgcttttt atatctctat ctgcctgaat attgcctttt ttaaacaggt
    16501 gttgcaggcg ctgccgctgg attcgctgca taacgtactg gttttcttgt cgatgccggt
    16561 tgtcgctttc agcgtgatta atattgtcct gacactaagc tctttcttat ggcttaatcg
    16621 accactggcc tgcctgttta ttctgattgg cgcggctgca caatatttca taatgactta
    16681 cggcatcgtc atcgaccgct cgatgattgc caatattatt gataccactc cagcagaaag
    16741 ttatgcgctg atgacaccgc aaatgttatt aacgctggga ttcagcggcg tgcttgctga
    16801 gctgattgcc tgctggctaa aaatcaaacc tgccacctcg cgtctgcgca gtgttctttt
    16861 ccgtggagcc aatattctgg tttctgtact gctgattttg ctggtcgccg cactgtttta
    16921 taaagactac gcctcgttgt tccgcaataa caaagagctg gtgaaatcct taagcccctc
    16981 taacagcatt gttgccagct ggtcatggta ctcccatcag cgactggcaa atctgccgct
    17041 ggtgcgaatt ggtgaagacg cgcaccgcaa cccgttaatg cagaacgaaa aacgtaaaaa
    17101 tttgaccatc ctgattgtcg gcgaaacctc gcgggcggag aacttctccc tcaacggcta
    17161 cccgcgtgaa actaacccgc ggctggcgaa agataacgtg gtctatttcc ctaataccgc
    17221 atcttgcggc acggcaacgg cagtttcagt accgtgcatg ttctcggata tgccgcgtga
    17281 gcactacaaa gaagagctgg cacagcacca ggaaggcgtg ctggatatca ttcagcgagc
    17341 gggcatcaac gtgctgtgga atgacaacga tggcggctgt aaaggtgcct gcgaccgcgt
    17401 gcctcaccag aacgtcaccg cgctgaatct gcctgatcag tgcatcaacg gcgaatgcta
    17461 tgacgaagtg ctgttccacg ggctggaaga gtacatcaat aacctgcagg gtgatggcgt
    17521 gattgtctta cacaccatcg gcagccacgg tccgacctat tacaaccgct atccgccgca
    17581 gttcaggaaa tttaccccaa cctgcgacac caatgagatc cagacctgta ccaaagagca
    17641 actggtgaac acttacgaca acacgctggt ttacgtcgac tatattgttg ataaagcgat
    17701 taatctgctt aaagaacatc aggataaatt taccaccagc ctggtttatc tttctgacca
    17761 cggtgaatcg ttaggtgaaa atggcatcta tctgcacggt ctgccttatg ccatcgcccc
    17821 ggatagccaa aaacaggtgc caatgctgct gtggctgtcg gaggattatc aaaagcggta
    17881 tcaggttgac cagaactgcc tgcaaaaaca ggcgcaaacg caacactatt cacaagacaa
    17941 tttattctcc acgctattgg gattaactgg cgttgagacg aagtattacc aggccgcgga
    18001 tgatattctg caaacttgca ggagagtgag taaatgaaaa ttctgattgt tgaagacgat
    18061 acgctgttat tgcagggact gattctggcg gcgcaaaccg aaggctacgc gtgcgatggc
    18121 gtgacaaccg cgcggatggc ggaacaaagc cttgaggacg gtcattacag cctggtggta
    18181 ctggatttag ggttacccga cgaagatgga ctgcattttc tcgcccgtat ccggcagaaa
    18241 aaatacaccc tgccggtact gatcctcacc gctcgcgata cgctgaccga caaaatcgcc
    18301 gggctggatg tcggtgccga tgactatctg gtgaagcctt ttgcgctgga agagttacac
    18361 gcccgtatcc gcgccctgct acgacgccat aataatcagg gcgaaagtga gctgattgtt
    18421 ggcaatctga cgctgaatat gggtcgccgt caggtatgga tgggcggtga agagttgatt
    18481 ctgacgccca aagaatatgc tctgctgtca cggttaatgc tcaaagcagg cagtccggta
    18541 catcgggaaa ttctctacaa cgacatctat aactgggaca atgaaccctc gaccaacacc
    18601 ctggaagtgc atatccacaa tctgcgcgac aaagtgggca aagcccgtat ccgcaccgtg
    18661 cgcggctttg gctatatgct ggtcgcgaat gaggaaaact aattgaatct gatgcgtttt
    18721 ctgcgccgac ctatatcgct gcgccaacgg ctgatattga ccatcggggc cattttgttg
    18781 gtgtttgagc tgatcagcgt cttctggcta tggcatgaaa gtaccgagca gattcagctg
    18841 tttgagcagg cgctacgcga caatcgcaac aacgatcgcc acatcatgcg tgagatccgc
    18901 gaggcggtcg ccagcctgat tgtccccggc gtctttatgg tcagcctgac gctatttatc
    18961 tgctatcagg cggtacgccg catcacccgc ccgctggcgg agctgcaaaa agagctggaa
    19021 gcgcgcaccg ccgacaactt aacgcccatt gccattcaca gcgccaccct cgaaatcgaa
    19081 gcggtggttt cggcgttgaa cgatctggtc agtcgcctga ccagcacgct ggataacgaa
    19141 aggttgttta ccgctgacgt cgcgcacgaa ctgcgaacgc cactggcggg ggtgcgtttg
    19201 catctggaac tgctggcgaa aacgcatcac attgatgtag caccgttagt ggcacggctt
    19261 gatcagatga tggagagcgt ctcccagctg ctgcaactgg cgcgtgccgg acagtcattt
    19321 tcttccggta attatcaaca tgtaaaactg ctggaagatg tcattctccc ctcgtatgac
    19381 gaactcagta ccatgctcga ccagcgacag caaaccctgc tattgccaga gagcgccgcc
    19441 gacatcaccg ttcagggtga tgcaaccctg ctgcggatgt tattgcgaaa cctggtagaa
    19501 aacgcccatc gttacagccc gcaaggcagc aacattatga ttaagctgca agaagatggc
    19561 ggagcggtca tggcagttga agatgaagga ccaggtattg atgagagtaa atgcggggag
    19621 ttgagtaaag cgtttgtacg tatggacagc cgttatggcg ggattggtct ggggttaagt
    19681 attgtcagcc gcattacaca gttgcatcac gggcagtttt tcctgcaaaa ccggcaagag
    19741 acttccggca cgcgggcctg ggtacggctg aagaaagatc agaacgtggc aaaccagata
    19801 taaagaaagc tgctgaccac cagcacgctg aacacggtag ttaaactttc ataaacacgg
    19861 tttttcattg cactctcctc cgttaacctg gaggagagta tgcgcgtcag agattaagtg
    19921 aaccttaaga gttcaattgg cctgataaga cagcgtcaca tcaggccatc cgtttcagct
    19981 tattcatcaa ttcgcggatg ttgctgcacc aggcgggtac gtttcgcctg caaatcggca
    20041 atctcgtggt caatattatc gattttctgc tcgatattat cgtaatgctc gacgagaatt
    20101 tccttcgctt cctgtatatc tgacgccgcc ggtgtcgcac ctttcaacgg acgatttgcc
    20161 gtctctttca tggttacgcc ggtgattaaa ccaatcaccg ccactaccat caggtaatag
    20221 gcaggcatca tcagattctg cgagctttcg accagccagg ccgccagtgt tggcgtcaga
    20281 ccggcaacca gcaccgaaat attaaatgcc gccgccagcg cgctgtaacg gatatgcgtc
    20341 gggaacatcg ctggcaaggt agaagccata acgcccataa agcagttaag gatcaccgcc
    20401 agcatcagta acccggcaaa aatcaggccg atgacgttac tgttaatcag aataaacgcc
    20461 gggatcgcca acacaaacag tgcaacacta ccaagtagca caaacggacg acggccaaaa
    20521 cggtcactca gcaagcccat caccggctgg acaaacagca taccgatcat aatggcgata
    20581 ataatcagca ccccgtggtc ttcggagtaa tgcaggttat gcgacaaata actcggcata
    20641 taggtcagca acatgtagta agtcacgttg gtggcaatta ccagaccaat acatgtcaac
    20701 aggctgcgcc agtatttagt ggcaatctct ttaaacgaga ctttcgggcc atcctgcaaa
    20761 ccttcgcggt cgccctgttc cagtttatca acatgctgct ggaacgccgg agtttcttcc
    20821 agcgcatggc gcaggtaaag cccgataatc cctaacggca gagcaataaa gaacggaata
    20881 cgccagcccc agtcgaggaa gttcgcttcg ccgacaatgg tcgaaattaa caccaccacg
    20941 cccgcaccca gcacaaaccc ggcaatagaa ccgaagtcca gccagctgcc cataaagcca
    21001 cgtttacggt caggggagta ttccgcaaca aatatcgacg ccccggtata ttcaccaccg
    21061 accgagaaac cttgtgccat cttacagatc aacagcagaa tcggtgccca aataccaatc
    21121 gtgtcgtagg acggtattaa gccaatacag aacgtactga tcgacataat cacaatagtg
    21181 atagcgagga tcttctggcg accatattta tcgcccaaca taccaaagaa gagtccgcca
    21241 agcggtcgaa tcagaaaggg aacggagaaa gtggcaagtg cagcaaccat ctgcacgctg
    21301 gggtcagccc ccgggaaaaa aactttacct aatgcgtaag caacaaaacc ataaacacca
    21361 aaatcaaacc attccattgc attacccagt gatgctgcgg taatggcttt acgcagttta
    21421 ccgtcatcaa taatggtgac atcacgaagg gtaatcggtt ttactttttt ccttttcagc
    21481 atagctttcc tcgcagagtt ggcccatttc cgcaaacgcc agaaaaatca gcgcgacggc
    21541 cgcaaaaggg gagcgggaat tgcaggatgc aatctctgta acaagcgtag cgggtttgct
    21601 tacaccctcc ggttaaggaa tgtacaatca aaccagttga cgcctgaatt gggcagttaa
    21661 tgaccttttt accctaccag tgtttaaatt tgtgatcaac ttcacacatg gatttcgttt
    21721 aggactcatt gatgtaactg attatttacg ccaaatttac agagaagatt tcctcgacca
    21781 ttgcccccac caaacgctgc gctgaacaga gtcgtggcat ccccagcgcg acagtttgcc
    21841 agcgttgggt cactgaccag caaacgggtt gtttatctgc cgtggtccac tttcccagcc
    21901 aacttaaaaa atcagcatga tccccgcccg tcggaacggt cgaagaggca aaccactgat
    21961 ggtaatagtg acgcgtagtc ggtgccgcat tgatactctc tgccagatgt tgtgtcgctt
    22021 tctcgcgcaa atcacccagc aacaacacca ccgcttgcgg gcttcccaga cggcaggcat
    22081 cgccaaatgc accccagcgc aactcctcca gaacgataaa acagcgccca ggcaacgacc
    22141 aggcgttata cgccccagca cgccagcggg taaaaatttg ctcgctctga tcacctgcct
    22201 gcaccagtag gcctcgttgc gttaacgcct tttccttcca gcgacaacgg gctgaaaacc
    22261 actcatacaa ggcggcaatt tgctggggga gctccgggaa agcagagtgc tggcggtgat
    22321 ccattttttg cagcagcgcc agatgctgcg cgatctgcgt cagcgccaga tgaccgggat
    22381 ccagcatcga cgccagtttt tccaccattg tcagtcgcag gctaagcggc gcatttaaca
    22441 tgccgccgtt cgtccatgtc tgaagctgca cacgcgaaag aagttcctgc atcagtcgtt
    22501 cacgaaactg ctgctgatgg gcttgaggat ggcgggcatc atcgccctgc gccagatcca
    22561 ctaaaaagcg cggatgaacc gactccagca cgcgaccggg gccgtccagt aacgtcttgg
    22621 tcaatatcgt tctgccgtga aaagtgtttg aatatcatcg cgtaacagct gagcgtcggt
    22681 gtaaatccag ctgtgagtca tcacagtctg ctgcaattgc tgctgcatca gcctgaccac
    22741 cgattcattt tgttgacgca gagccagact ttcgcgtaaa cgcgtctgta attctgtcaa
    22801 acatgaagcg aactcagcga aaaaagtatt catgcctgcc gtaacagatt catcgacctg
    22861 ctctgccaga actttagcca tttgttggca atagagatcg acttctgcgc ttaatgctcg
    22921 ttgcaacaca ctgtaatcaa ccgtttctgt cggggatttc tcatttcccc gtccccagtc
    22981 gggctgattc aaccagcgcg aaaaagtctc acgcacaacg cctaaacgcg tgctctgctc
    23041 gtccgttgct tcctggcgcg aaatgacttc actgaacagc tggcgagtgt tgaagtgggg
    23101 aactacgccg tgaaaaacag gaaaatgaaa cccaggacga aaccctgact cgctcaattc
    23161 cattttgact tgttgctcaa tggggcgaat aacatcggtt aacacccggc aaagggtgga
    23221 ttccatctcg gcaaaacgca gcgtaaagtc gcgactgatg gtgttctgcg ccgtctgtaa
    23281 cagtgtctca cagcgggtac gaatctcgct taacggctcc gaatcatcct gaaacaaggc
    23341 ggctaactgc gcattcaccg catcttgctg ttgacgcaga aagtggttgg cggaggtcag
    23401 ggccagctcg atttcatgtt taatctcgcc gctcacctgc gcctgattga gttgcaatag
    23461 ctgcaaactt tcttcgacct gatggatatt ttgccgcaat tgttcacaag cgacgtttaa
    23521 cccgtgcgca cgaaaatcca ggtattcccg cgcctgctgc gcgtaattca acagtttatg
    23581 cgcagcagat cgcagagcat acaatgaggc gttagcgtaa gcggcatgaa gcaacgcctg
    23641 aattggctgg gcgaacagcg aatcttccca caactgatcg gcagcatgac gaatatgttc
    23701 gaggtccgcc agatcggcat gacgccagcg cctgccgagc gcggcatggg caaaatcttc
    23761 cacccagcgt tgttgctctg gcgctggtaa cttaccgttg ttggctaact catagcgcgc
    23821 ccgattcgcc aggtagcccc acatcgacga caccgggaat atctgctgtg gcgtaataca
    23881 gcctttcatc agcgtcccgg aaatcagtgc ccgcacctgg tcggcgtcgt cactgttacg
    23941 atcctgttga tcgaacttat tgaccagcac atacagcggc accgattgcc ccaccgccaa
    24001 aatcgcctca cggacctctt catcggagat cgatttcagt tgcgtataat ccagcaccgc
    24061 cagtaccgcc gaggcgcgtg ccagctgctg gttaagcatt ttttgcagat gcggttgccc
    24121 ggcttcattt ggcccggggg tatccagtaa cgtcaactga ccgggataac tttccagccc
    24181 cgccagatgg acaaactcca cttcaatcac gggaatatgc tcaatggcgg cgtaagcaga
    24241 aaaaggaaaa tcgacgtcca gcgccttcgc cagtcgcact aaatcattca aacttttcag
    24301 acaatgaaaa ataggctggg cacccagata atatttttcg aaagcgacgc cattttcgat
    24361 ccgctgcata agcgcacgca tatctttatc tatttccagc acatcggtca gatgcttaat
    24421 atcgcaatca cgcaggcgct gttgtaattg ttgaattaaa caatcgattg gcgcgacatg
    24481 tgaaaaatgc agtaccggtt ccttttgccc gggcgtatgg cgaataagcg tcggcagcgc
    24541 agtcattggg cgattacgat taggcagaac ctccgtacca acaatggcat taatggtggt
    24601 tgatttccct gctttcatgg taccgacaat tgccagcacc atttccagtc gggaaatttt
    24661 acacaactca ttattcagca tcgcgtgacg ttcggcgata ttaggctgac tccagggtaa
    24721 agccagttgt ggcgcgtcgt ctccgggtac agagagaggc attttttcca gtaactgcaa
    24781 ctgttggcga gaaagctgta acaggcgttc agcctcctga cttaactcat acagggtctg
    24841 tgtgtacata gaaaattctt ccttaaagca aattttgtta cttttattta gccagattgt
    24901 ttttgagttc tgttttcggc ttttataatt actgcaagaa ataattttat atttagtgtg
    24961 ttgttttttt atcagaataa ataacgtctt ctgatgcgtt taaaacgtca gaaagataaa
    25021 aatatcatgt gaataaaaaa aagaacaagt agagcattaa cattatctta aataataaat
    25081 agaggcaaaa agattatttt ctttttgcgt ttcctttcaa atgaaaacga tcgtcgtcta
    25141 aaatcagcag tacccccgac aaactcaggg attttgtgta taattgcggc ctttttcggc
    25201 aatctgccgt tttttggcgc ttttgccctg ctgacttttg aggaaatcca catgtcatta
    25261 ccacactgcc caaaatgcaa ctccgaatac acttacgaag ataacggcat gtacatctgc
    25321 ccggaatgtg cctacgaatg gaacgacgca gaacctgcac aggaaagcga cgagctgatc
    25381 gttaaagatg ctaacggcaa tctgctggct gacggcgaca gcgttaccat cattaaagat
    25441 ctgaaggtga aaggtagctc ttcgatgctg aaaattggca ccaaagtgaa aaacatccgc
    25501 ctggttgaag gcgaccataa catcgattgc aaaatcgacg gttttggtcc gatgaaactg
    25561 aaatctgagt ttgtgaaaaa gaactgattg tattgtgatc ggtaagccgg ataaggcgct
    25621 cgcgccgcat ccggcaacga tgccgactgc ctgatgcgac gcttgcgcgt cttatcaggc
    25681 agcctctcat ctgtataaat ttcgaactac acttaactgg cttctcttaa ctgaggtcac
    25741 catcatgccg ttaagtccct acctctcttt tgccggtaac tgttccgacg cgattgccta
    25801 ttatcaacgt acgttgggcg cggaactgct ctataaaatc agcttcggcg aaatgccgaa
    25861 atcagcgcag gacagcgccg agaactgccc ttccggaatg caatttcccg ataccgccat
    25921 cgctcatgcc aacgtgcgca ttgccggaag cgacatcatg atgagcgatg ctataccgtc
    25981 aggaaaagcc agctactccg gctttacgct ggtgctcgat tcgcaacagg tcgaagaagg
    26041 aaaacgctgg tttgataacc ttgccgctaa cggaaaaatc gaaatggcct ggcaggaaac
    26101 tttctgggcg catggctttg gcaaagtcac cgataaattt ggcgtaccgt ggatgattaa
    26161 tgtcgtcaaa caacaaccaa cgcaataacc cgccgggagg cccgccctcc cgcactgtca
    26221 tcgaattccc gttaactctt catctgttag tcacttttaa ttaaccaaat cgtcacaata
    26281 atccgccacg atggagccac ttttttaggg aggctgcatc atgcaaacga ttatccgtgt
    26341 cgagaagctc gccaaaacct tcaatcagca tcaagcgctg catgcggttg atctgaacat
    26401 tcatcacagt gaaatggtgg ctctgcttgg gccgtcgggt tccggcaaat ccaccctttt
    26461 acgtcattta agcggtttga ttaccggcga taaatccgcc ggtagccata tcgagctact
    26521 gggccgcact gtccagcgcg aaggccgcct ggcccgcgat atccgcaaaa gccgcgccaa
    26581 caccggctac atctttcaac aattcaacct ggtgaaccgc ctgagcgtac tggagaacgt
    26641 gctgattggc gcgctcggca gcacgccgtt ctggcgcacc tgttttagct ggttcaccgg
    26701 cgagcagaaa cagcgcgcgt tacaggcgct gacccgcgtt ggcatggtgc attttgccca
    26761 tcagcgcgtt tccaccctct ccggcggcca gcagcaacgt gtggcgattg cccgtgcgct
    26821 gatgcagcag gcgaaagtga ttctggccga tgaacccatc gcctcgctgg acccggaatc
    26881 agcgcgcatc gtgatggaca ccctgcgcga catcaaccag aacgacggca tcaccgtggt
    26941 cgtcacgctg catcaggtgg attacgccct gcgctactgc gaacgcatcg tcgccctgcg
    27001 tcaggggcac gtcttctacg acggcagcag ccaactgttt gataacgaac gttttgacca
    27061 tctctaccgc agcattaacc gcatcgaaga gaacgcgaaa gctgcctgat atccccatca
    27121 ttgaggaaaa cgaatgaacg ctaagataat tgcctcgctg gccttcacca gcatgttcag
    27181 cctcagcacc ctgttaaccc cggcacacgc cgaagagcag gaaaaggcgc tgaatttcgg
    27241 cattatttca acggaatcac agcaaaacct gaaaccgcaa tggacgccgt ttttacagga
    27301 tatggagaag aaactgggcg tgaaggtcaa cgccttcttt gccccggact acgcgggcat
    27361 tatccagggg atgcgcttca ataaagtgga tattgcctgg tacggcaacc tgtcggcgat
    27421 ggaagcggtg gatcgcgcca atggccaggt cttcgcccag acggtcgcgg cggatggatc
    27481 gccgggttac tggagcgtgt tgatcgtcaa caaagacagc ccgatcaaca acctgaacga
    27541 tctgctggcg aagcggaaag atctcacctt cggcaatggc gatcctaact ccacctctgg
    27601 cttcctcgtc cccggttact acgtcttcgc taaaaacaat atctccgcca gcgacttcaa
    27661 gcgcaccgtc aacgccgggc atgaaaccaa cgcgctggcc gtcgccaaca agcaagtgga
    27721 tgttgccacc aacaacaccg aaaacctcga caagctgaaa acctccgcgc cggagaagct
    27781 taaagagctg aaggtgatct ggaaatcgcc gctgatccta ggcgatccga tcgtctggcg
    27841 caagaatctg tccgaaacta ccaaagacaa gatctacgac ttctttatga actacggaaa
    27901 aacgccggaa gagaaagcgg tgctggaacg cctgggctgg gcgccgttcc gcgcttccag
    27961 cgacctgcaa ctggtgccga ttcgccagct cgcactgttt aaagagatac aaagcgtgaa
    28021 aggcaataaa gggctgaatg agcaggacaa gctggcgaaa accaccgaga ttcaggcgca
    28081 gctggatgac ctggaccgcc tgaacaacgc gttaagcgcg atgagttcgg tgagtaaagc
    28141 ggtgcagtaa atcgtaggtc ggataagacg ccccggcgtc gcatccgaca atgtgtaggc
    28201 gttgatgccg gatgcggtgc aagcacctta tccggcctac agaccggagc caaacatgca
    28261 aaccatcacc atcgccccac ccaagcgtag ctggttctcg cttctgagct gggccgttgt
    28321 tctcgacgtg ctggtcgtct cgtggcaggg cgcggaaatg gccccgctta cgctgattaa
    28381 agacggcggc aacatggcaa cctt
//
LOCUS       DAJTSI010000045        28386 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000045 DAJTSI010000000
VERSION     DAJTSI010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28386
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.045"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="OO327_001688"
                     /pseudo
     CDS             complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="OO327_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
     gene            complement(1023..2045)
                     /locus_tag="OO327_001689"
     CDS             complement(1023..2045)
                     /locus_tag="OO327_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6018262.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2059..3561)
                     /locus_tag="OO327_001690"
     CDS             complement(2059..3561)
                     /locus_tag="OO327_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6018263.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="OO327_001691"
                     /pseudo
     CDS             complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="OO327_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4967..5497
                     /gene="ppa"
                     /locus_tag="OO327_001692"
     CDS             4967..5497
                     /gene="ppa"
                     /locus_tag="OO327_001692"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR6018264.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5877..6218)
                     /locus_tag="OO327_001693"
     CDS             complement(5877..6218)
                     /locus_tag="OO327_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR6018265.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="OO327_001694"
     CDS             complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="OO327_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR6018266.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="OO327_001695"
     CDS             complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="OO327_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR6018267.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11936..12574
                     /gene="msrA"
                     /locus_tag="OO327_001696"
     CDS             11936..12574
                     /gene="msrA"
                     /locus_tag="OO327_001696"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR6018268.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12897..14240
                     /locus_tag="OO327_001697"
     CDS             12897..14240
                     /locus_tag="OO327_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6018269.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14302..14508)
                     /locus_tag="OO327_001698"
     CDS             complement(14302..14508)
                     /locus_tag="OO327_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR6018270.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14833..15387
                     /locus_tag="OO327_001699"
     CDS             14833..15387
                     /locus_tag="OO327_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR6018271.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15450..16388)
                     /locus_tag="OO327_001700"
     CDS             complement(15450..16388)
                     /locus_tag="OO327_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018272.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16600..17340)
                     /gene="cysQ"
                     /locus_tag="OO327_001701"
     CDS             complement(16600..17340)
                     /gene="cysQ"
                     /locus_tag="OO327_001701"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR6018273.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17530..19473
                     /gene="cpdB"
                     /locus_tag="OO327_001702"
     CDS             17530..19473
                     /gene="cpdB"
                     /locus_tag="OO327_001702"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR6018274.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(19599..19979)
                     /locus_tag="OO327_001703"
     CDS             complement(19599..19979)
                     /locus_tag="OO327_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR6018275.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            20068..20928
                     /gene="qorB"
                     /locus_tag="OO327_001704"
     CDS             20068..20928
                     /gene="qorB"
                     /locus_tag="OO327_001704"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR6018276.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            21037..22002
                     /locus_tag="OO327_001705"
     CDS             21037..22002
                     /locus_tag="OO327_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6018277.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            22132..22794
                     /gene="ytfE"
                     /locus_tag="OO327_001706"
     CDS             22132..22794
                     /gene="ytfE"
                     /locus_tag="OO327_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR6018278.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22839..24251)
                     /gene="cycA"
                     /locus_tag="OO327_001707"
                     /gene_synonym="dagA"
     CDS             complement(22839..24251)
                     /gene="cycA"
                     /locus_tag="OO327_001707"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR6018279.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24559..25179)
                     /gene="fklB"
                     /locus_tag="OO327_001708"
     CDS             complement(24559..25179)
                     /gene="fklB"
                     /locus_tag="OO327_001708"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6018280.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            25398..25943
                     /locus_tag="OO327_001709"
     CDS             25398..25943
                     /locus_tag="OO327_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HCR6018281.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25927..26574)
                     /locus_tag="OO327_001710"
     CDS             complement(25927..26574)
                     /locus_tag="OO327_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR6018282.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26786..27577
                     /locus_tag="OO327_001711"
     CDS             26786..27577
                     /locus_tag="OO327_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HCR6018283.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27587..>28386
                     /locus_tag="OO327_001712"
     CDS             27587..>28386
                     /locus_tag="OO327_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR6018284.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDIN"
BASE COUNT         6968 a         7553 c         6997 g         6868 t
ORIGIN      
        1 tccttaactg atcggcatta ctccacacgg agaggcattt ttatcgctgc gcaatgttga
       61 ctcttgtcac tggcgaactc tgacgattct cccacagcac cgttaaccca cgctgtaatg
      121 ctataaaaat aaacaacaaa ataccgatgg cgattttcgt ccactaggaa ctcagcgtgc
      181 cgtcaaagtt gatgtaagtc tggatcagtc cctgaattgc cacgccaaaa agcgtcccaa
      241 gcaccgtccc gacgccacca cttaacaacg taccgccaat caccaccgac gcgatggcgt
      301 ccagttccac acctacgccc gccagtgcat atccggcttg ggtataaatc gagaagacaa
      361 tccccgccag tgttgccagt ccggtggaga gcatataaat gcgaatagtg gtgctgcgag
      421 tagaaatccc catcaggttc gccgacgtcg cgttgccgcc aatagcgtac acctgattgc
      481 caaaacgggt acaatgcgca aggaaaatac cgataaccac caccgccagc atcaacagcc
      541 ccatcgcact aaggcgaccg ccgccgggga ttttccacgc aaggcttgag agcgtgtcat
      601 aaatgggatg gtttatcgga atcgactctt ccgaaacgag atagctgacg ccgcgcaaaa
      661 agaacatccc cgcaagcgta atgataaatg ccgggatctt cagggcgtcg atcagaagcc
      721 ccataaatgc gccgaaggca cagcccatca ccagcactag cggaaacgcc agcagcggcg
      781 agaggccgaa atcgccaacc acttttgcca gaaacacgcc agtaaaggcg atcaccgaac
      841 cgacggagag atcgataccg ccagagagga tcacaaaggt catgccaacg gcgattatcc
      901 caagaaaggc gttatcggtc aggatattgc agatcaccct tgtggaagca aaaccgggaa
      961 actgggtcag gcagtagaga tagcccagca caaagacgcc gatggtgatc atcagcggca
     1021 agttacgttt tatcatggct acgtactcct ttaatcagac tgataaagcg ttgcgactga
     1081 acaatcagca cacaaagcac caccaccgct ttcaccacct ggttcatctc cggcggaaag
     1141 cccgaaagca aaattccggt gttcatcccc tgaataatca gcgcccccac taccgagagc
     1201 agcaggttaa agcgcccgcc catcagcgat ccgccgccaa tcaccaccgc gagaatggcg
     1261 tccagctcca gccataatcc ggcgttgttg gcatcggcac cgcgaatatc cgccgccacg
     1321 ataatgcccg caatcgccgc acatagcccg ctcaacacgt aggtgagcat gacgatgatt
     1381 cgcgtgttta ccccggcatt ttttgccgcc cgaatgttga taccaacgac ttcgataaac
     1441 atccccagcg ccgttttgcg ggtcaacagc cagaacagga gaagcgtcag caccgcgata
     1501 atgaccggcg ttggcaggaa caacagcgat ccgctgccaa accatgagag atccggcgag
     1561 ttaaacgtga cgatctgccc ggcggtgatc agttgcgcca cgccgcgccc ggcgaccatc
     1621 aggatcaggg tggcgacaaa cggctgaatt ttgaggatcg ctaccagtat gccgttccac
     1681 aatcccgcca ggatgccggt gcccagggcg cttaacaaaa caatcggcag gctgaatccc
     1741 gcgaccgtca tcgcagccgt tgtggctccg gcgatagcca tcaccgcccc cacagagaga
     1801 tcgataccac cggtggcgat caccagcgta ataccaatcg ccagtaacgc aacgggagct
     1861 gcacggttaa gaatgtcgat agggctaccg aacaaacgcc catcctgaag caccacctgc
     1921 cagaaatgcg gggctaccag gctatcgacc agcagcacca gcaacagtgc cgccagctgc
     1981 ggcatccccg tcggccagtg aaaacgcctt tttggcggcg tagtgtcagg gagagattga
     2041 ggcatcacac tgttctcctt acgccgcaat ggcgttcatg atcgccggaa cggaaagctg
     2101 tgccagcggg atctccgcca cctgtttgcg atcgcgcatg atgatcaccc ggtcggcata
     2161 gcccaccagc tcttccagtt cggaggagat caccagcagc gccagaccgt cagcacacag
     2221 cgtttcaatc aggcggatga tctcggcgtg ggcaccaaca tcaatgccac gggttagctc
     2281 atcaaggatc agaaattgcg gtcgggtcag tagccaacgt gaaagcagca ctttttgctg
     2341 attgccaccg gagagaaatt caatcggttg ttcagttgaa ggtgtgcgaa tgccaagctg
     2401 gcggataaag cgttcggcaa tctcttgctg ctctttgcgg gaaatgggtc gtagccagcc
     2461 gcgctgggcc tggagagcga ggatgatatt ttcccgcacc gaggcggcgg cgatgatgcc
     2521 atcggttttc ctgtcttccg ggcagaatcc gatgcccagt actgaagcct gatgtggcga
     2581 tcgcagggtt tgcggtttgc ctttgatcaa cgccgtgccg ctgtcagcag gtttgatacc
     2641 gaagatcact tcggcggttt cggtacgtcc ggatcccagc aagccagcca gaccgacgat
     2701 ctcgccgggg cgcacttcga gatcaaacgg tgctatcgtt ccttttttgc cgtaattttt
     2761 gaacgcggca acgggtttgt cgctcaacaa tgttcgcccg gcacgctgta gcgcgtgggt
     2821 atccagctcg cgccccagca tcatttttac cagttcgatc tgcggtagct cgcgcgtttc
     2881 ccgacagcct acgaaactgc cgttgcgtaa gacggtgatc cgatcgctga cctgatagac
     2941 ctgatcgaga aagtgagtga caaagatcag gctgacgccg cgatcgcgca actgacgcat
     3001 caggccaaat aatagctcca tttcctgggt gtcgaggctg gcggtgggtt catcgaggat
     3061 cagcactttg gcggagagat cgatagcccg gcaaatggcg acgatttgct gcatcgccac
     3121 cgaaaaacgg ttaagcggtt cgcgcacgtc gagggagaaa ccgtaagatg ccatcagttc
     3181 ggtggcgcgc ttttccatct ctttacggcg tagaaggccg aaacgtttgg gttcgcgacc
     3241 tatgaataga ttatcagcaa ccgacatatt gggtagcagg ttgacttcct gatagacggt
     3301 gccgataccg agttgttgtg catgggcggt atttttcggt gagatagtct ggccttccag
     3361 ccagatggtg ccgcgatcgg cgtggtatac accagttaat gctttgatta gcgttgattt
     3421 tcccgccccg ttttcaccaa gcagcgccat gatttcgcca cggcgcaggc tgaaatcaac
     3481 gttgtctaac gctttgacgc cggggaaaaa tttacttaat ccttcggtgc ggaggatctc
     3541 ctggtgttgg tcggtcgtca tggtttcccc cggtgtaaag cgtgctccca gtaggcatga
     3601 taagacgcgg taagcgtcgc atcaggcttc agtgcaccat cgccggatgc ggcgcgagcg
     3661 ccttatccgg cctacgaatc ggcatcaggc atagcaaccc tcaatacccc atatttttct
     3721 tcttctctaa ctcttctttt gcggtatcag gcaggtaaag ggtggatttg gttaacgtca
     3781 gcttttcagg catggtgccg tcttttttgt atttctccag cgcatcgaaa gcgggtcccg
     3841 ccatgttcgg cgtcagttca acactggcgt tcgcttcgcc atccatcatc gctttgtaga
     3901 tgtccggtac accgtcaatg gaacccgtga ggatatcttt gcccggtttc aggcccgctt
     3961 ctttaattgc ctgaattgca ccaatcacca tgtcgtcgtt atgggcgtaa accatgcaga
     4021 tgtttttgcc gttgttttcc gctttgataa agctctccat gacttctttg cctttactgc
     4081 gggtgaagtc acctgactgc gagcggataa ttttgatatt tggcgcattc ttaatggctt
     4141 cggcaaagcc tttcttacgg tcaatggcga cgctggcccc aacggtgccc tgcagctcca
     4201 ccacgttgca tggtttgcca ttcacttctt ttaccagcca gtcaccgatc aacttacctt
     4261 cgaggatgtt gtcggcagta acggtggtca tatagagaga tttgtctttc acatcgatgg
     4321 aacggtcgag caagaagacc gggatttcgg catctttcgc ctcttttaat accggttccc
     4381 aacccgtcgc aaccactgga gcgataaaga tcgcatccac cccttatgca acgaaggaac
     4441 gtaccgcttt aatctggttt tcctgctttt gctgaccatc ggcaattttc aacgtaattc
     4501 cgcgtttttc ggcctcactt ttcgccacat tggtttctgc ggcgcgccag ccggattccg
     4561 atccgacctg cgaaaatcct acggttaatg gagcggccaa cgccatagac gacatggctg
     4621 ccgagactgc agagactaca agtaagcgtt tccacatagg gttgtcctcg tcggggtaat
     4681 tattattggt gaaaagatgt tcgcgaaaaa actatagaca attcgttatg taacggattg
     4741 cgttacatca cacttcaaaa aagtaaataa aacgttaatc acaagtttgt aatcgctttc
     4801 atctcactat gaaaaatgcg gctacggtta tggattttcc tgctctgtat accgtcttaa
     4861 aactggcgaa aaaggaaaat gaagacgaaa acaagcaaag acattcggcg cgagttggct
     4921 ataatacttg gcacttgttt gccacatatt tttaaaggaa acagacatga gcttactcaa
     4981 cgtccctgcg ggtaaagatc tgccggaaga catctacgtt gttattgaga tcccggctaa
     5041 cgcagatccg atcaaatacg aaatcgacaa agagagcggc gcactgttcg ttgaccgctt
     5101 catgtccacc gcgatgttct atccgtgcaa ctacggttac atcaaccaca ccctgtctct
     5161 ggacggtgac ccggttgacg tactggtccc gactccgtac ccgctgcagc ctggttctgt
     5221 gatccgttgc cgtccggttg ccgttctgaa aatgaccgac gaagccggtg aagatgcgaa
     5281 actggttgcg gttccgcaca gcaagctgag caaagaatac gatcacatta aagacgtaaa
     5341 cgatctgcct gaactgctga aagcgcaaat cgctcacttc ttcgagcact acaaagacct
     5401 cgaaaaaggc aagtgggtga aagttgaagg ttgggaaaac gcagaagccg ctaaagctga
     5461 aatcgttgct tccttcgagc gcgcaaagaa taaataagtt cttctagcgc aataaccctg
     5521 aacgccgggc ttcggttagt aagggttttt ttatgcccgc gataaataaa ctctctattc
     5581 caccatcatt attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca
     5641 ccattttgct gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt
     5701 caaagcgtgc ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg
     5761 attgcagata tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa
     5821 aatcgcgaga tacatcgcat aaaacaacgc cacccgaagg aggcgtgctt ttattattac
     5881 ttatccctgt ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga
     5941 taaacgtaca tccatgcact cccatacggc gtctgaatca actggcgcgc gtattcaccg
     6001 cccctggtgc gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca
     6061 ccgtgtaccg ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg
     6121 tagttatcga tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct
     6181 tgtttgtggc gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga
     6241 gcaaatccag tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc
     6301 gataacgtaa cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca
     6361 gacctggcaa tacatagccg ctgaccacta cctgggagga gtcgcctact cccagggtgt
     6421 cgagcgctaa attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt
     6481 gcgcaacccc cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct
     6541 ccagcccttg tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag
     6601 agaagatctc cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcgattacat
     6661 cgtcttctgt agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg
     6721 gaccagagaa cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc
     6781 gcccttcagg gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt
     6841 taagatcgcc cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa
     6901 cgtggacaat caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca
     6961 ggttatcgtt aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt
     7021 gcaccacgat gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg
     7081 tagcctcgaa tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc
     7141 gcactttact gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg
     7201 catcaccgtt caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc
     7261 gcataccgtt aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc
     7321 cgtcgatatc cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac
     7381 cacccagacg caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg
     7441 ggtttatcat cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc
     7501 cttgcggatc ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg
     7561 tccagcccaa ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg
     7621 ccaccggcag cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg
     7681 tgatactgcc ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg
     7741 tgaagatacc gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga
     7801 agcggttgag attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc
     7861 acagttccgc attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat
     7921 tgcggtaatc cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaagc
     7981 gggtattgct aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat
     8041 taagctgccc ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc
     8101 tgcctttggc attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa
     8161 cgcgtacgtc gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa
     8221 cctgcgccac ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt
     8281 gtggcgcttc caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg
     8341 gcagcgcgtt atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc
     8401 ccagctcgcc tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca
     8461 tccactgcat ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga
     8521 cgttaccggt cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac
     8581 cgcgggtttt aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt
     8641 taatgccatt aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg
     8701 ccttgagttc agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct
     8761 gttcattacc tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg
     8821 ccgtacgcat agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat
     8881 ccgcctgata ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca
     8941 cgttgagtgg caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg
     9001 gaccggaaag gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct
     9061 tcaccttttc acctttcaac ggctccacgt tcagtgtgct gttgagggtg atatccaccg
     9121 gccagttgtc tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga
     9181 tatccagggc gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc
     9241 gcacggtgat gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt
     9301 tcaggttaag cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat
     9361 ctttcagcgt ttcgccgagc ggcttttcct ccggctgcgg attttcaatt ttcggttcga
     9421 ccacttcttc ctgcgccact tccaccactt tcggcagagc aatcagcagg cctttcagcg
     9481 acgtcggttt cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca
     9541 ccgacaccgt ggtgtcatca atcttgatgt tgacgttgtc cagcgccacc cgtgtcaggg
     9601 tgatgggata cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt
     9661 cagaaggagg cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat
     9721 taatacaaac gctactgttc cacaggcact caagaccgac ccccagatgc agattacccg
     9781 cttttaccgc cacgcctagc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc
     9841 cgccggtgac cttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa
     9901 gatgcaggcc gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa
     9961 cgataaccac gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat
    10021 gtaaaactgt aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat
    10081 tggcccgacc ggcgattccc agcgtacgcc gaccccggta ccggttttaa agtcgctgcg
    10141 gcgaatatcg cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt
    10201 cacgttgtac tggtattcca gcgatccggt tatcaacttc gaggcccctt tcaggtcacc
    10261 gttggcgtat ttcggggcga tagatttgta tttgtagccg cgaatactgc gatcgccccc
    10321 gacgaagaaa cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag
    10381 cgtgccgcgt gtcacaaagc gatggcgatc gtacagtgtg cggatccaga cgtgctgcgc
    10441 ctggaaaacg gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta
    10501 gcgttgcgag tctccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat
    10561 caccccagga taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag
    10621 gctccagcgc aggttaatgg cacgctgcca gccgctggag agatcccagt agcgagaagc
    10681 caccagcgta gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg
    10741 caccaaataa tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag
    10801 ggtctgttcc ggcgcggaaa tactggtact ggtggtcaaa ctgtgaccgt atgagttcat
    10861 ccacggcttt ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc
    10921 ggtttcgatg gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt
    10981 ttcgcgcgct ttatcaaatt gtggagccac caccaccgag ttaaaccagc cggtagcaga
    11041 aagtcggcgg ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg
    11101 caccagattt tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa
    11161 gcggtaacgt tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc
    11221 aatgcccagc tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact
    11281 ggttaaggac tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg
    11341 gcgagtatcg agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac
    11401 atcggtgccg ccaattaaca ccggcacacc tggcgtgact ttggcgatca atacctgccg
    11461 ccctttcttt ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc
    11521 tttcagacct tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac
    11581 ttcatcactt tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa
    11641 cccctcgacc tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct
    11701 tacacagcat aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctgcc
    11761 cctggaacgc cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta
    11821 aaccccaaat atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa
    11881 tacgcctctt gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag
    11941 tttatttgat aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc
    12001 gatgcccgta gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg
    12061 aatggagatt gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca
    12121 gttacccggc gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac
    12181 ttatcgggaa gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga
    12241 tccttccgtc atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc
    12301 ccagggaatg cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct
    12361 gaccccagaa caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct
    12421 tgccgccgat gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta
    12481 tgccgaagat gaccaccagc aatatctgca taaaaaccca tatggttatt gtggaattgg
    12541 cggaattggc gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt
    12601 gataaagtgc tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat
    12661 cgcccaaatt caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca
    12721 gtattgcatt tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc
    12781 tatactagcc actgaaaatg ccggttcact ttcttcgaat cggctttcaa tgtgtatttc
    12841 acacaaatta atcaactttc ccttccgagg gtctggcctg aaaggtcgga taagatatgt
    12901 taaacagtat tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg
    12961 agatctcgct tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata
    13021 taaatgccca acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc
    13081 aaatcggtct gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc
    13141 cagcttttca cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct
    13201 ttattctctc tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga
    13261 aacgcatcgg tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct
    13321 tctgcctgta cgtttgcacc ccgctggtgt ggttcttcaa cggcctggcg aacatgatct
    13381 tccgtatttt caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg
    13441 cggtagtgga agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg
    13501 aaaacgtctt tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg
    13561 tgatttggtt tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc
    13621 cgcactctaa gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt
    13681 ctaaagacct gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac
    13741 aaattcgcaa cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt
    13801 ttaaaaccgc aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga
    13861 tcatcaccct caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg
    13921 aagagcagat tgtcgcccgt gatgagaact catggctgat tgacggcggc accccaattg
    13981 acgacgtcat gcgcgtgctg gatattgacg agttcccgca gtcgggcaac tacgaaacca
    14041 tcggcggctt tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat
    14101 tcgccggcta caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg
    14161 tgacccggat cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata
    14221 aagaagaaag cgtcgcgtaa cccccagaaa catcaacggc tcctgaatca ggagccgtct
    14281 tattactgca tagcactttg gttaagccat ctctgtttgc agacgcataa cctgacggtt
    14341 aacttcggac atcactgaca aatgcagctt atccttcact tttgggataa gaatcttacc
    14401 cttatcaaat tcaaaagcgc caatgtcctt gatgtataac cgtccacgga acaggatctt
    14461 tacgtacttc gccacctgaa gtgggttgta ccgttggaaa attttcattg ttatctcctg
    14521 ctgagtatta cgcctgtgcg gtgccacaat cggcccaact attatgaggc gcaaatttta
    14581 atgcttagtg actatagact atccgggcaa tgtttccacc gcgtataact ttttttacct
    14641 aaaggttaca attattcaga attatctttt taccgaagcg cggtcttcag tataagcatt
    14701 catttttcat atgatttgtg cgcttgaccg caaactggca tcacacttgc gggaaattcg
    14761 ataaatagca catatgatta aaactcagac ccaagtggtc ggatcacctg catatcataa
    14821 gaaggaaaca ccatgaccct acgcaagatt ctggcactca cctgcctgct gttgccgatg
    14881 atggcttccg cacatcagtt cgaaatcggt cagcgagtgc cgccgattgg cattaccgat
    14941 cggggcgagt tggtgcttga taaagatcag tttagctaca aaacctggaa cagcgcgcag
    15001 ttagtgggaa aagtgcgagt actgcaacat attgctggtc gcacctctgc aaaagagaaa
    15061 aacgcgacgc tgattgaagc gattaaatca gcgaagttac cgcacgatcg ttaccagacc
    15121 accaccattg ttaacaccga cgacgcgatt ccgggttccg gcatgtttgt gcgcagcagt
    15181 ctggagagta ataaaaagct ttatccctgg tcgcagttta ttgtcgatag caatggcgtc
    15241 gcgcgcggtg cctggcagct ggatgaagaa agttccgctg tagtggtgct ggataaagac
    15301 ggtcgcgtgc aatgggccaa agacggggcg ctcacaccgg aagaggtgca gcaagtgatg
    15361 gacctgctgc aaaagttgct caaataatgg caataacata aattctggaa aaataatttt
    15421 cccctatcgt tatctatggg ataggggaat taagtctctg agttcttaca ccgaactaat
    15481 actggtaacg ctagttgctc ttttttaaac gcctcaatgg tcgatttata tttctcgcaa
    15541 atcttttctt tggtaatatt actatctgcg gccagattcg cacttactat cgcatccact
    15601 tgttcggcta attcgacttg ttggcaacct gcattaacca gaaaattata aataagagag
    15661 taatcttcat cctccaaaac aacagaggaa ccattacgaa aacgttcatc ccctccatag
    15721 attttaagcg caaagcccaa agattctatg actatggcaa aattatcatc aattattttt
    15781 tcgtaaatat accatccatc gcattcttta attccaccgc ctggtatttg gagttcaaat
    15841 ttaccccgtt ttaatttgct tactacatcc tccaataagg cataaccgaa agatacgtca
    15901 ccaaacgcct ggcgaatata acctttaatc ctttttggta tgtcagactg ttttttctgc
    15961 aaaatatcac aggcatacaa tcttctgttt tgttctaacg gaaactcgcg gtttttgaaa
    16021 tccatgaatg agttatactc atttattgtt atgggaattt cttcgtattc aaacccactg
    16081 cctcgcaatg ttattgtata ttcagcaccg ttatctttaa ggctgaaact gataagaaaa
    16141 ttatccatgg cattaacagt ttcatttttt gatgaaagca cttgataact atttttatca
    16201 atctgtgttg cgcaacgaaa ggactcccgc aaagttaaca gactatcata attcgaaacc
    16261 caatcttttt tcttaaatgc atgctgaata ttattaaaaa aatcgtttga atgtgttttt
    16321 gtatgcgatg actttacaaa taactccacc gcaccaataa aattattgtg tctgatcctg
    16381 ggtaacatat ggctacctta ttttatttgg ttattttatg tcgccattaa atatctcatt
    16441 atagaagtaa actatcaaaa aacgctcatt attaaaatgt aacttactag caattgaatt
    16501 tttgcacttt aaatacaatc tctttttatt tataactttt taggttggga taaatcagga
    16561 tggcagaatc aggaaataca ctgtttctgc catctgaatt tagtaaatgg acactctgaa
    16621 ccccggattc aggaacgact cgcgcggagt gtaatccagc ggtttaccct gccagtcgtg
    16681 aacgtgcgct ccggcagctg cagccacagc atgtccggcg gcggtgtccc aaatattcgt
    16741 tggtccgaag cgcgggtaca gctgcgcctg tccttccgcc accaggcaga atttcagcga
    16801 agagccgatg gacgtggtct gatgttcgcc aagctgttgc agatactctt tcagctccgc
    16861 atctgcatgg gaacggctga taaccaccag cggcgggcgc gcatcgcgga cctgaatcag
    16921 cttgcgcaca ccgcactctt ctttccaggc cttgccttct gccgcgctgt acattacgtt
    16981 cattaccggc gcatacacca cacctaaaat cggtttgcca tggtcaatga gcgcaatgtt
    17041 aacggtgaat tcgccattac gtttaataaa ctctttagta ccatccagcg ggtctaccag
    17101 ccagtaacgc tgccagtgct gacggacttc ccaaccggga ggatcttctt cagaaaggac
    17161 cggaatatcc ggtgccagcg tacgtaaacc gtccatgata acggtgtgag cggcaatatc
    17221 cgctgccgtt accggagaat tgtccgcttt gctgacgacg tccatcggtt tcgtcccgtc
    17281 gtagacctgc ataatggcat cgcctgcatt ccgtgcaagc tggcatactt gatctaacat
    17341 ttctccacct cgtctctgtg agcggtgtta acttattgtt ttacttatac cctatcgtta
    17401 atgaatgcgc caactgtgat agtgtcatca ttttcaaagc gtaaaattgt ggcattcttc
    17461 actgttctat aagtaaggcg tttattctcc cttttctttc gtattcccga tgataaaagg
    17521 atgtccctga tgattaagtt tagcgcaacg ctcctggcca cgctgattgc cgccagtgtg
    17581 aatgcagcga cggtcgatct gcgtatcatg gaaaccactg atctgcatag caacatgatg
    17641 gatttcgatt attacaaaga caccgccacg gaaaaattcg gactggtacg tacggcaagc
    17701 ctgattaacg atgcccgcaa tgaagtgaaa aacagcgtac tggtcgataa cggcgatttg
    17761 attcagggga gtccgctggc cgattacatg tcggcgaaag gattaaaagc aggtgatgtt
    17821 catccggttt ataaggcgct gaatacgctg gattatacgg tcggtacact cggcaatcat
    17881 gaatttaact acggtctgga ttacctgaaa aatgcgctgg cgggagcgaa attcccttat
    17941 gtaaatgcca acgtcattga cgccagaacc aaacagccaa tgtttacacc gtatttaatt
    18001 aaagataccg aagtggtcga taaagacgga aaaaaacaga cgctgaagat tggctatatt
    18061 ggcgtcgtgc cgccgcaaat catgggctgg gataaagcta atttatccgg aaaagtgacg
    18121 gtgaatgata ttaccgaaac cgtgcgcaaa tacgtgcctg aaatgcgcga gaaaggtgcc
    18181 gatgtcattg tcgttctggc gcattccggg ctgtctgccg atccgtataa agtaatggcg
    18241 gaaaactcag tttattacct cagtgaaatt ccgggcgtta acgccattat gtttggccat
    18301 gctcacgccg ttttcccggg taaagatttt gctgatatcg aaggggctga tatcaccaaa
    18361 ggcacgctga atggtgttcc ggcggtaatg ccgggcatgt ggggcgatca tcttggggtg
    18421 gtcgacttac aactcagtaa tgacagcggt aaatggcagg tgacgcaggc gaaagcggaa
    18481 gcacggccga tttacgacat cgccaataaa aaatccctcg cggcggaaga cagcaagctg
    18541 gtagaaacac tcaaagccga tcacgatgcc acacgccagt tcgtcagcaa gccaatcggt
    18601 aaatctgccg acaatatgta tagctatctg gcgctggtgc aggacgatcc gaccgtgcaa
    18661 gtcgtgaaca acgcgcaaaa agcgtatgtc gaacattaca ttcagggcga tccggatctg
    18721 gcaaaactgc cggtgctttc agctgccgca ccgtttaaag ttggtggtcg caaaaatgac
    18781 ccggcaagct atgtggaggt ggaaaaaggc cagttgacct tccgtaatgc cgccgatctt
    18841 tatctctacc ccaatacgct gattgtagtg aaagccagcg gtaaagaggt gaaagagtgg
    18901 ctggagtgct ccgccggaca gtttaaccag attgctccta acagcacgaa accacagtca
    18961 ctcatcaact gggatggttt ccgcacttat aactttgatg ttattgatgg tgtgaattat
    19021 cagattgatg ttacccaacc cgcccgctat gacggcgagt gccagatgat taatgccaat
    19081 gcagagcgga ttaagaacct gacctttaat ggcaagccga ttgatccgaa cgctatgttc
    19141 ctggttgcca ccaataacta tcgcgcttac ggcggcaaat ttgccggtac gggcgacagc
    19201 catatcgctt ttgcttcacc ggatgagaac cgctcggtgc tggcagcgtg gattgctgat
    19261 gagtcgaaac gtgcggggga aattcatccg gcggcagata acaactggcg tttagcaccg
    19321 atagctgccg ataagaaact ggatatccgt ttcgaaactt ccccgtcaga taaagccgca
    19381 gcgtttatta aagagaaagg gcagtatccg atgaataaag tcgcgaccga tgatatcggg
    19441 tttgcgattt atcaggtgga tttgagtaag taaaacactt ctttttcggc ctataaatca
    19501 accgcatccg gcatttattg gcggatgcga tgctgccgta tcttatccac cctacaagcc
    19561 atgaaccgta gaccggataa gcgcagcgca tccggcagtt atgccgcacg ttcatcccgc
    19621 accgccaaca cctcgggcaa attcaactca atccagtccg ccagtgcagc aaccttttcg
    19681 ctcacctgct cgcccagcgg cgtgaggcta tattccacat gcggcggcac caccggatac
    19741 gcgatacggt taagaaaacc atcctgttct aacgcctgta acgactgcgc aagcatcttt
    19801 tcactcaccc caccgatttt gcgccgcagg tcgctaaagc gatgagtacc ttcgcgtagc
    19861 gccaccagaa tcaacacccc ccaacggctg gtgacgtgtt tcaacacctc gcgcgacggg
    19921 cactgttccg caaagagatt accctctttc agttgttgtg acaggctaac ctgactcatt
    19981 tcatacttac ctttttgcac gtacttacta aaagtaagtt taggtgttag cgtatttaaa
    20041 cacaagacaa aacgatggag acttcccatg atcgctatta ctggtgccac tggccaactt
    20101 ggtcactata ttattgaatc cttgatgaaa acggttcctg ccagccaaat agtggctatc
    20161 gttcgtaatc cggcaaaagc ccaggccctg gcagcacaag gcattaccgt gcgtcaggct
    20221 gactacagcg atgaagccgc actgacatct gcacttcagg gagtggaaaa actactgctg
    20281 atctcttcca gcgaagtggg tcaacgtgcc ccgcagcatc gtaatgttat taatgccgca
    20341 aaggcggctg gcgtgaaatt tatcgcttat accagcctgc tacatgcaga tacctccccg
    20401 ctcggcctcg ccgatgagca catcgagacg gagaaaatgc tggctgattc tggcatcgtt
    20461 tacaccctgc tgcgcaacgg ctggtacagc gaaaactacc tcgccagcgc cccggcagca
    20521 ctggaacacg gcgtatttat cggtgcggct ggcgatggca aaatcgcctc agcaacgcgg
    20581 gcagattatg cgacagctgc ggcacgcgtg attagcgaag ccggtcacga aggcaaggtt
    20641 tacgaactgg cgggcgatag tgcctggacg ttgacacagt tagcggcaga gctgaccaaa
    20701 cagagcggca aacaggttac ctatcaaaat ctgagcgaag ccgatttcgc cgcggcactg
    20761 aaaagcgtcg gactgcccga cggactggcg gatatgctgg cggattctga cgttggcgca
    20821 tcgaaaggtg gtctgtttga taacagcaaa acgcttagca aattgattgg ctgcccaacg
    20881 acaacgttag ccgaaagcgt aagccatctt tttaatgtta ataactagtt aattaaagtg
    20941 gcatcctccc gcatcctctc tgataatgac gggatgccgg gagcaatcat gtctgcttcc
    21001 tgaacgtttc ttctgacaga ccaatggatg ccagtaatga ttagcggcgt gctgtacgcc
    21061 ctgttagcag ggttgatgtg ggggcttatt tttgtcgggc cgttgatcgt gccggaatac
    21121 ccggcgatgt tgcagtcgat ggggcgttat ctggcgttag ggttaattgc gctacccatt
    21181 gcctggctgg gacgcgtgcg tctgcgtcag ttggcgcgtc gggactggct taccgccttg
    21241 atgctcacta tgatgggcaa cctcatttat tacttctgcc ttgccagtgc cattcaacgt
    21301 actggcgcgc ctgtttccac gatgattatc ggcaccctgc cggtggtgat tcctgtcttt
    21361 gccaatctgc tttatagcca gcgcgacggc aaactcgcgt ggggaaaact cgccccggca
    21421 ctgatttgta ttggcatcgg cctggcgagt gtgaatattg ctgagttaaa ccacggactc
    21481 cccgattttg actgggcacg ttatacctct ggcatcgtgc tagcgttagt ttccgtggtc
    21541 tgctgggcat ggtatgccct gcgcaacgcc cgctggctgc gggaaaatcc cgacaaacat
    21601 ccgatgatgt gggcgacggc gcaggcgctg gtcacgctgc cggtttctct catcggctat
    21661 ctcgtcgcct gttactggct gaatatacaa acgccggact tctccttacc ttttggcccc
    21721 cgtccgctgg tgtttattag tctgatggtt gcgatagccg tgctttgctc atgggttggc
    21781 gcactctgct ggaacgtcgc cagccagcga ttaccgacag tgattctcgg gccgctgatt
    21841 gttttcgaaa cgctggcagg tttgctgtac acctttttac tccgccagca aatgccgccg
    21901 ctaatgacgc tgagcggtat cgcgctgtta gtgattggcg tggtcatcgc ggtcagagca
    21961 aaaccagaaa aacctttaac tgaatctgtc tcagaaagtt gacatgctgg cagtgagtta
    22021 aataagcctc tgctacgtaa gggttatagc ttttgcctta aaggtgtatt taaaatacat
    22081 cttatcttat taagaatgag gtatcagcta tttaagaatg aggtatcagc tatggcttat
    22141 cgcgaccaac ctttaggtga actggcgctc tctatccctc gcgcttcggc tctgtttcgt
    22201 aaatatgata tggattactg ctgtggcggt aagcagacgc tggcgcgcgc ggcggcacgt
    22261 aaagaactgg atgttgaggt cattgaagct gaactggcaa agcttgctga acaaccgatt
    22321 gagaaagact ggcgtagcgc cccgctggca gaaatcattg accatatcat cgtgcgctac
    22381 cacgatcgtc accgcgagca gctgccggag ctgatcctgc aagcgactaa agtcgagcgc
    22441 gttcacgccg acaaaccgag cgtgccaaaa gggctgacaa aatacctgac catgctgcat
    22501 gaagagcttt ccagccacat gatgaaagaa gagcagatcc tcttcccgat gatcaaacaa
    22561 ggcatgggca gccaggcaat ggggccaatc agcgtaatgg aaagcgagca cgatgaagcg
    22621 ggcgaactgc tggaagtgat caaacacact accaataacg tcacaccgcc gccagaagcc
    22681 tgcaccacct ggaaagcgat gtataacggc attaatgaac tgattgatga cctgatggat
    22741 cacatcagtc tggaaaacaa tgtactgttc ccacgcgccc tagcgggtga gtgatctaaa
    22801 agctggatgg cattgcgcca tccagcatga taatgcggtt atttccgcag ttcagcagcc
    22861 cgcttcttac caataaacag ccagcccaac cccagcgcga taaaccacag cggggtaacc
    22921 agcagcgcct ggcgagtgtc atcttccagt gtcagcaaca cgaccacgaa cacaaagaac
    22981 gccatacata cccagcacat cagcttgccg agcggcatct tgtagatcga cttttcatgc
    23041 agatgaggac gctgtttgcg gtacaccagg taagagcaaa ggataatcgt ccagacgaac
    23101 ataaacagaa tcgcggaaac ggttgtaatc atcgtgaacg cgccaatcac gctaggattc
    23161 acatacaaca tcaccacgcc gccgagcaga cagatacacg agaaggtcaa ccctttcgcg
    23221 ggtactgcgc gcttagaaag tttagcgaac gctttcggcg caacaccttc ctgcgccaga
    23281 ccaaacagca tacggctggt agagaagaca ccgctgttag cggaagacgc cgcagaggtc
    23341 agcaccacaa agttgatcac gctggcagcc gcaggcaaac ctaccaacac aaacagctca
    23401 acgaacgggc ttttctccgg gactaccgaa ctccacggtg tcacggacat aatcacaatc
    23461 agcgcgaaga cgtagaacat aatgatacgg atcggaatgg agttaatcgc gcgtggcagt
    23521 gatttctccg gatctttggt ttccgcagct gttgtaccta ccagctcaat ccccacgaaa
    23581 gcgaaaaccg ctatctggaa tccggcaaag aagccactta aacctttcgg gaaccagccg
    23641 ccgtcattcc acaaatgcgc gaatgacgct tccacaccgg tcggtgactg aaagtgcatc
    23701 gccaccatga ccaggccgac gacaatcagc gacacgatgg cgacgatttt gatcatcgca
    23761 aaccagaact ccatctcacc gaacattttc acggtggcaa ggttgagcgt cagcagcagc
    23821 actatcaccg ccagcgaggc gacccagtcg gagagatcgg ggaaccagaa ctgagcataa
    23881 gccgtgatcg ccaccacgtc tgccataccg gttacaaccc agcagaacca gtaagtccag
    23941 ccggtgaaat atcccgccca cggccccagt aaatcggaag cgaagtcact aaaagatttg
    24001 tattccagat tcgaaagcag caattccccc attgcccgca tcacgaaaaa gagcataaaa
    24061 ccaatgatca tataaacgaa aatgatcgac ggcccggcaa ggctaatcgt tttgccggac
    24121 cccataaaca acccagtacc aatggcacct ccaatggcaa taagctgaac atgtcggttt
    24181 gtgagattgc gccgtagcga ctgttcagcc ggagcctgat catcggcaac gacttttacc
    24241 tgatctacca tgttttttct tcctgtacct gtctgtgttg ttcaggctct acggccttta
    24301 gtcagtctat gatacgacga atctgtatcg ggactcatcg atattaggta acaatacgcg
    24361 ggatgaatac taagatttag atctaatgtt aattttatgt ttaaagtgag tgtcatatca
    24421 cggtgataac gcgaaacagc tcacaaaaat acacgcttac ggggtctttg caacacaaaa
    24481 tagcgccgtt tggcaaactg aagggtttat tgctgaatgc ctgctcccct ctggttcgag
    24541 gggaacagaa tgcgctgctt agaggatttc cagcagttcg acttcaaaca ccagggtgct
    24601 gaacggaggg atggatgcac ctgcgccgcg ctcgccatat gccagttcct gcgggatagt
    24661 cagttcccat ttagaaccta ccggcatcag agtcagtgct tcaatccagc cagggatcac
    24721 gccattaacc gggaattcag cgggttcacc acgagcaacg gagctgtcaa acacggtgcc
    24781 gtcgatcagt ttaccggtgt aatgaacacg aacgcggtcg gtacgtgccg gaattgcgcc
    24841 ttcaccctgg ttgatcacgc ggaattgcag gccagattcg gtgctattca caccttcttt
    24901 tttggcgttt tcttccaggt atttcacgcc ttcagcagcc atcgcctgga aacgctgacg
    24961 acgaacggca tcggcgcgtt cgtggatttc acgcagcgcg cgatgcacca catcaaccgg
    25021 cacagccgga tgtttgcctt ccagcgcatc ggcaatacct gcaaccagtg cttctggcag
    25081 cagcccttcc aggccagatt cactcagttg ttgccctacc tgcaaaccaa tgccgtagct
    25141 tgcttgcgct tcgatggtgt caaaagttgg ggtggtcatc gtatttcctt tcttagggta
    25201 taaaagtagc gggcagcata tcagccacac ttctttgggt aaaactttgt cgcaggaatg
    25261 atgacaaatc ccggggaaaa agaaacaata gcaatatccc gtatgaatat aataaaggcg
    25321 ggtttaccac ggacatcata gcgttagctt attcataggc tatgattgag gaacaagacg
    25381 tggagcagga ggaaaccatg cccgggcgct ttgaactaaa accaaccctg gagaaagtct
    25441 ggcacgcgcc ggacaatttt cgcttcatgg acccgctgcc gccgatgcat cgtcggggga
    25501 tcattattgc cgccattgta ttggtggtcg gatttctgct tccatctgat gatacgccca
    25561 acgcgtcggt ggtgacgcgt gaagcgcagt tagatattca gtcgcaatca caaccgccga
    25621 cagaagaaca gccgcaaact acccaaacgc aaccattcca gccagatagc ggaattgata
    25681 atcaatggcg ctcttaccgt gtcgaacccg gtaaaacgat ggcgcagctg ttccgcgacc
    25741 acggactgcc cgctaccgat gtctatgcaa tggcgcaggt agaaggcgcg ggtaagccgc
    25801 tgagtaattt gcaaaatggg cagatggtga aaatccgcca gaacgccagc ggcgtggtga
    25861 cgggcttaac cattgatacg gggaataatc aacaggtcct gtttacccgc cagccggatg
    25921 gcagttttat tcgtgcgcgg tagatgaagg gtcaaccaaa agccagctaa ggattgtctc
    25981 tttgatgacc ttacgtctgg cttccagtgc ctggggggaa accatactga tatgataaac
    26041 taggccaagc gtatgctggt tgatcaaata atatccccca agcgcagcaa tattaatatt
    26101 cacctgcaat gggtcgatac ctggcttaaa aatattgtat ttcttccctt catccaataa
    26161 ggattccatt aactgcaagt gagcatggtt aatttctaag agacgctgtg attttgcata
    26221 atgcacacct ttactttgat tttcactatg cacaattttt aaaaaccaag gattagcaat
    26281 ataataatcc catgtaaaat caataatttt ctcaatggct ttttgtggcg gaagatctga
    26341 gaaatcaagg gccttttctt tttgtcgaat atcgttccag gtatattcca gcacttcgat
    26401 aaataaattt tctttattac caaagtggtg ataaaccgtc tgtttactac accctgctgc
    26461 ctttacaata ttatctactc tggccccttc ataaccatat tcggcaaatt cattaaccgc
    26521 acatagcagc aatttttcct tcaggctctg tacgttgctg gtatcaatat acataacgct
    26581 tccccacgac ctcttcgtgg tgactatcat aacctgattt gatttcagct tcagaaaaaa
    26641 gggcaaaaac ttgtactttc atcaggttat caaatgtcct gtgatcgcca tcgcatatca
    26701 accaactaga tggtttatct ttttatcctc cagtcgcaag ctaatcatca ccaaggaaac
    26761 aaaattcaaa aaagagagct attgtatgaa atccactcgt cctgttgccg tgattaccgg
    26821 agccactcgc ggtattggaa agggctgtgc gcaggagctt gctcgtggcg ggtttaacct
    26881 gctgattaac gatcgtcccg atgccgacag cgtagaaaaa ctgcatatca cacaacaaga
    26941 atgtttggcg gaaggcgtgg aagtgatttg cttccctgct gatgtcggtg atctatctct
    27001 gcatgaggag atgctcgacg cggcgcaaaa ccagtggggg cggctggact gtctgctcaa
    27061 taacgcgggg atttcagtga aaaaacgcgg tgacctcctc gacctcgaac cagacagctt
    27121 cgatcaaaac attgccatta acacccgcgc gccgttcttc cttgctcagg cattcagtaa
    27181 gcgcttactg gcacagccga aaccggaagc ggagctgccg caccgctcga ttattttcgt
    27241 cagttccatc aacgccatca tgctggcgat gaatcgcggc gaatacacca ttgccaaaac
    27301 cgccgtttct gccgctgctc gtctgtttgc tgctcgcctt tgtaatgaac aaattggcgt
    27361 ttatgaagtg cgccctggtc tcatcaaaac tgatatgact attccggcaa cggcttatta
    27421 cgacgaactg attgccaaag gtctggtgcc gtggggacgt tggggttatc cggctgatat
    27481 tgcttccacc gtccgcgcga tggcggaagg taaactgatt tacacctgtg gtcaggcggt
    27541 cgcaatcgac ggcggcttaa gtatgccgcg cttctgaggc tgaatcatga gagatctgtt
    27601 acaacgcccg gatttattca gtatcaatac cgcgacactg ggctataaaa cgccgctacc
    27661 cgccattatt gatgcctgcg ccgcacgcgg tattggcgca attgccccct ggcgcaggga
    27721 gttgcagggt gaagatctcc agcaaatcac ccgccagctt gccgccagta acatgagcgt
    27781 ttccggtttg tgccgtagca cttactacac cgcgccgaca ctggcggaac gcaaactggc
    27841 gattgatgat aaccgccggg cgctggacga cgcagcggtg cttaacgccg cctgttatat
    27901 gcaggtggtt ggcggtctgc caacgggcac taaagatcta tatgaagcgc gagaacaagt
    27961 gaaacaaggg attcgccagt tgctgccaca ttcgaaagat gtcggcgtgc caattgcgct
    28021 ggagccactg cacccgatga cagctgccga tcgctcttgc ctgtgcacgt tacgtcaggc
    28081 gctggactgg tgtgatgaac tcgatcccga tggtgagttt ggcctcggcg tggcggtgga
    28141 tgtttatcac gtctggtggg atccggatct cgccagtcag atcctgcgtg ccggaaaacg
    28201 catcctcgcg tttcatgttt ccgactggtt aatgccgacc actgatctgg tcaatgaccg
    28261 tggtatgccg ggagatggcg tcatcaatat tccgtcaatt cgtcggctgg ttgaaaacgc
    28321 cggatttaat ggtgctattg aactggaaat attctcgcct tactggtggc agaaagatat
    28381 taacag
//
LOCUS       DAJTSI010000046        27651 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000046 DAJTSI010000000
VERSION     DAJTSI010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27651
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.046"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..794
                     /locus_tag="OO327_001713"
     CDS             <1..794
                     /locus_tag="OO327_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6018285.1"
                     /translation="LPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDD
                     DNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFA
                     LIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQF
                     VVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYL
                     SGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            805..1896
                     /locus_tag="OO327_001714"
     CDS             805..1896
                     /locus_tag="OO327_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6018286.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1939..2799)
                     /gene="rsmI"
                     /locus_tag="OO327_001715"
     CDS             complement(1939..2799)
                     /gene="rsmI"
                     /locus_tag="OO327_001715"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR6018287.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2864..4900
                     /gene="lpoA"
                     /locus_tag="OO327_001716"
     CDS             2864..4900
                     /gene="lpoA"
                     /locus_tag="OO327_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR6018288.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4858..5253
                     /locus_tag="OO327_001717"
     CDS             4858..5253
                     /locus_tag="OO327_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR6018289.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5273..5863
                     /gene="diaA"
                     /locus_tag="OO327_001718"
     CDS             5273..5863
                     /gene="diaA"
                     /locus_tag="OO327_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR6018290.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5873..6448
                     /gene="yraP"
                     /locus_tag="OO327_001719"
     CDS             5873..6448
                     /gene="yraP"
                     /locus_tag="OO327_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR6018291.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6562..7602)
                     /locus_tag="OO327_001720"
     CDS             complement(6562..7602)
                     /locus_tag="OO327_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6018292.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7675..8310)
                     /locus_tag="OO327_001721"
     CDS             complement(7675..8310)
                     /locus_tag="OO327_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR6018293.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8438..8956
                     /gene="yhbO"
                     /locus_tag="OO327_001722"
     CDS             8438..8956
                     /gene="yhbO"
                     /locus_tag="OO327_001722"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR6018294.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8936..9379)
                     /locus_tag="OO327_001723"
     CDS             complement(8936..9379)
                     /locus_tag="OO327_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR6018295.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9430..9732
                     /gene="yhbQ"
                     /locus_tag="OO327_001724"
     CDS             9430..9732
                     /gene="yhbQ"
                     /locus_tag="OO327_001724"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR6018296.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9719..10222)
                     /locus_tag="OO327_001725"
     CDS             complement(9719..10222)
                     /locus_tag="OO327_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6018297.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10216..10740)
                     /locus_tag="OO327_001726"
     CDS             complement(10216..10740)
                     /locus_tag="OO327_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR6018298.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10949..11944
                     /locus_tag="OO327_001727"
     CDS             10949..11944
                     /locus_tag="OO327_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6018299.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11953..12831
                     /locus_tag="OO327_001728"
     CDS             11953..12831
                     /locus_tag="OO327_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6018300.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12912..13919
                     /locus_tag="OO327_001729"
     CDS             12912..13919
                     /locus_tag="OO327_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR6018301.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14037..15281)
                     /gene="mtr"
                     /locus_tag="OO327_001730"
     CDS             complement(14037..15281)
                     /gene="mtr"
                     /locus_tag="OO327_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR6018302.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15435..17324)
                     /gene="deaD"
                     /locus_tag="OO327_001731"
     CDS             complement(15435..17324)
                     /gene="deaD"
                     /locus_tag="OO327_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR6018303.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17317..17397)
                     /gene="yrbN"
                     /locus_tag="OO327_001732"
     CDS             complement(17317..17397)
                     /gene="yrbN"
                     /locus_tag="OO327_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR6018304.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17504..18388)
                     /gene="nlpI"
                     /locus_tag="OO327_001733"
     CDS             complement(17504..18388)
                     /gene="nlpI"
                     /locus_tag="OO327_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR6018305.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18497..20632)
                     /gene="pnp"
                     /locus_tag="OO327_001734"
     CDS             complement(18497..20632)
                     /gene="pnp"
                     /locus_tag="OO327_001734"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR6018306.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20879..21148)
                     /gene="rpsO"
                     /locus_tag="OO327_001735"
     CDS             complement(20879..21148)
                     /gene="rpsO"
                     /locus_tag="OO327_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR6018307.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21297..22241)
                     /gene="truB"
                     /locus_tag="OO327_001736"
     CDS             complement(21297..22241)
                     /gene="truB"
                     /locus_tag="OO327_001736"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR6018308.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22241..22642)
                     /gene="rbfA"
                     /locus_tag="OO327_001737"
     CDS             complement(22241..22642)
                     /gene="rbfA"
                     /locus_tag="OO327_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR6018309.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22806..25454)
                     /gene="infB"
                     /locus_tag="OO327_001738"
     CDS             complement(22806..25454)
                     /gene="infB"
                     /locus_tag="OO327_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR6018310.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25479..26966)
                     /gene="nusA"
                     /locus_tag="OO327_001739"
     CDS             complement(25479..26966)
                     /gene="nusA"
                     /locus_tag="OO327_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR6018311.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26994..27446)
                     /gene="rimP"
                     /locus_tag="OO327_001740"
     CDS             complement(26994..27446)
                     /gene="rimP"
                     /locus_tag="OO327_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR6018312.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
BASE COUNT         6599 a         7638 c         6944 g         6470 t
ORIGIN      
        1 ggttaccgcg tagcctggtt tcctatcaga tgaccagcca gaaagatcgc ccaacccaac
       61 atgaaatgcg tcttgatggc tcactgctgg atgatggtcg cctgagctat agcctggaac
      121 aaagtctgga tgacgataac aaccataaca gtagcctgaa cgccagttac cgttcacctt
      181 atggaacctt cagtgccgga tacagttacg gtaatgacag cagccaatac aattacggcg
      241 ttaccggcgg cgtggttatc catcctcatg gcgtgacgct ctcgcaatat ctgggcaacg
      301 cttttgcgct tatcgatgct aacggggctt ctggcgtgag gatacaaaac tatccgggga
      361 ttgctactga tccctttggc tatgcagtgg ttccttatct cacgacttat caggaaaacc
      421 gtctctcggt agatactacg cagctgcccg ataacgtcga tcttgaacaa acaacacagt
      481 ttgtggtgcc caacagaggt gcaatggtag cggcgcgttt caacgccaat atcggttatc
      541 gcgtacttgt tacagtcagc gatcgcaacg gtaaaccgtt gccctttggc gctcttgcca
      601 gcaacgatga tacggggcaa caaagtatcg tcgatgaggg cggcatacta tatctctctg
      661 ggatatcgag taaatcacaa agctggactg tacgctgggg aaatcaggca gatcaacaat
      721 gtcagtttgc ttttagtaca ccggattcag aaccaacaac ctctgtatta caaggcacag
      781 cgcagtgcca ttaaggataa aaaaatgaaa agagcgcctc ttataacagg acttttgttg
      841 atatccacat cctgcgctta tgcctcctca ggagggtgtg gagccgacag cactagcggt
      901 gcgacaaatt acagcagtgt ggttgatgat gttacggtga accagacaga taacgtgaca
      961 ggacgggagt ttacctctgc aacgctaagt agcactaact ggcaatacgc ctgttcctgc
     1021 tctgcgggta aggcagttaa acttgtctat atggtcagcc ccgtacttac caccactgga
     1081 catcagacag gatattacaa actcaatgac agcctggata ttaaaaccac attacaggca
     1141 aacgacattc caggactcac aaccgaccag gttgtctctg ttaacacccg attcacacag
     1201 ataaaaagca gcaccgtata ttctgctgca acccaaacgg gtgtttgcca gggtgacacg
     1261 tctcgttatg gacccgttaa tattggtgca aataccacct ttaccctgta tgtcaccaag
     1321 ccatttctcg gctcgatgac cattccgaaa acggatattg ccgtcattaa aggcgcgtgg
     1381 gtcgatggaa tgggaagccc gtctacaggt gacttccatg atttagtcaa gttatcgatt
     1441 cagggaaatc tcaccgcccc acagtcgtgc aaaattaatc agggcgatgt tattaaagtt
     1501 aattttggat tcatcaatgg tcagaagttt accacccgca atgccatgcc agacggtttt
     1561 actccagtag actttgatat cacttatgac tgtggtgata cttcaaagat taaaaactcg
     1621 ttgcaaatgc gcatcgacgg tacaactggg gtagtagacc agtacaacct ggtcgccagg
     1681 cgaagaagtt cagacaatgt gcccgatgtc ggtattcgta ttgaaaatct cggcggcgga
     1741 gttgcaaata ttccttttca gaacggtatc cttcccgttg atccttccgg gcatggcacc
     1801 gtcaatatgc gcgcctggcc agttaatctg gtcggtggtg agctggaaac aggaaaattt
     1861 cagggcacag ccaccattac cgtcatggtg cggtaaaaga agacgttgcg ttttgattaa
     1921 cgcaacgctt cggcactgtt acccctgctg ctccagcgca tacttataca gcgcattttt
     1981 cttcacgcca tgaatttctg cggccagcgc cgccgctttt ttcagcggca gttctgcctg
     2041 tagcagcgcc agcgtgcgca gggcatcggc gggtaagtct tcttcctgtg ctttatgacc
     2101 ttcgacaatc agcaccattt cgcctttgcg acggttttca tcttccttta cccacgccag
     2161 cagctcgcca acgggcgcgc cgtgaatggt ttcccaggtt ttggtcagct cacgcgccag
     2221 aaccacgtag cgggattcgc ctaataccgc aacgatatct tccaggctat ctaacagacg
     2281 gtgggtagat tcataaaaaa tcagcgtgcg cggctccgct tcaatggctt ttagcgcatc
     2341 acggcggcct tttgatttgg caggtaaaaa gccttcgtaa cagaaacggt cagatggtaa
     2401 acccgcggcg cttaacgcag tgatagcagc acacggtccg ggtagcggca ccacacgaat
     2461 ccccgcttca cggcaggtac gcaccagatg gtagccagga tcgttaatta gcggcgttcc
     2521 ggcatcggaa accagcgcaa tgttttgccc ctcttgcagc tttgccagca gcgtttcggc
     2581 tttttgttgt tcgttatggt cgtgcagcgc aaacaaccgg gcattaatcc caaaatgttg
     2641 cagcaataaa ccggtgtgac gagtatcctc ggcggcaatc agatcaacgg cctgtaatac
     2701 ctctaacgta cgctgggtga tatccgccag attgccgatt ggcgtcggta caatgtaaag
     2761 ctggccctga gaattatccg ccgattggtg ttgtttcatt gtgtcgtccg tattgccgat
     2821 ttaatattga gcattgcgta aaaaaaatat cactggatac attatggtac cctcaacatt
     2881 ttctcgtttg aaagccgcgc gttgtctgcc tgttgttctg gcagccctga ttttcgccgg
     2941 ttgtggcacc catactcccg atcagtccac tgcttatatg cagggcacgg cgcaggctga
     3001 ttctgccttt tatcttcagc agatgcagca aagctctgat gataccagga tcaactggca
     3061 attactcgcc attcgtgcac tggtgaaaga aggtaaaacc gggcaggcgg ttgagttgtt
     3121 taaccaacta ccgcaagaac tgaacgattc tcagcgtcgc gagaaaacac tgctggcggc
     3181 agagattaaa ctggcgcaga aagattttgc tggcgcgcaa aacttgctgg cgaaaatcac
     3241 acctgccgat ttagaacaaa accagcaagc gcgttactgg caggcaaaaa tcgatgccag
     3301 ccaggggcgt ccttccattg atttactgcg cgcgttaatt gctcaggaac cgctgcttgg
     3361 cgcgaaagaa aaacagcaga atattgatgc cacctggcag gcgctctcct ccatgactca
     3421 ggaacaggcg aatacgctgg tgatcaacgc cgacgaaaat attctgcaag gctggctgga
     3481 tctgcagcgc gtctggtttg ataaccgtaa cgatcccgac atgatgaaag ccgggatcgc
     3541 cgactggcag aaacgttatc cgaacaatcc gggcgcgaaa atgctgccaa cgcagttggt
     3601 taacgtaaaa gcgtttaaac cagcctcgac caacaaaatc gccctgctgt tgccgctgaa
     3661 tggccaggca gcggtatttg gtcgcactat tcagcaaggc tttgaagccg cgaaaaatat
     3721 cggcactcag ccagtggcgg ctcaggtagc tgccgcacct gccgcagacg tagcagaaca
     3781 acctcagccg caaactgcgg atagcgttgc cagcccggca caagcctcgg ttagcgatct
     3841 gaccggtgat cagcctgcgg cccagccggt gcctgtaagc gccccggcga caagcaccgc
     3901 agcggtaagc gcacccgcaa atccatccgc agagctgaaa atctacgata cctcatcaca
     3961 accacttagc cagatcttaa gccaggttca gcaggatggc gcgagtattg tggttggtcc
     4021 gttgctgaaa aataacgttg aagagttgct gaagagcaac accccgctga acgtactggc
     4081 actgaaccag ccggagaata tcgaaaaccg cgtcaatatt tgttacttcg cgctttcacc
     4141 ggaagacgaa gcgcgcgatg cagcgcgtca tattcgtgac cagggtaaac aagcgccgct
     4201 ggtgctgatc ccacgcagtg cattgggcga tcgcgtagcc aatgcgtttg cgcaagagtg
     4261 gcagaaactg ggcggcggca ccgttctgca acaaaaattt ggttccacca gcgaattacg
     4321 cgcgggtgtt aacggcggtt ctggtattgc tttaacgggt agcccgatta ctcccagagc
     4381 gacaaccgac tccggcatga cgaccaacaa tccaacgctg caaaccacgc caaccgatga
     4441 ccagttcacc aataatggcg gtcgtgtcga tgcggtctac attgtggcaa cgccgggtga
     4501 aatcgctttt atcaaaccga tgatcgccat gcgtaacggt agccagagcg gtgcaacgct
     4561 gtacgccagt tcccgcagcg cacaaggcac cgcaggcccg gatttccgac tggagatgga
     4621 aggcttgcag tacagcgaaa tcccgatgct ggcaggcggt aatctgccgt taatgcagca
     4681 ggcgctcagc gcggtgaaca acgattattc gctggctcgc atgtatgcga tgggcgttga
     4741 tgcctggtcg ttggcaaatc atttctcaca aatgcgccag gttcagggtt ttgaaatcaa
     4801 cggtaatacc ggaagtctga cggctaaccc ggattgcgtg attaacagga agttatcatg
     4861 gctacagtac caacaaggtc aggtagtccc cgccagttaa ccaccaaaca gaccggcgat
     4921 gcgtgggaag cacaagcgcg tcgctggctg gaaggcaaag gactgcggtt tatcgccgct
     4981 aacgtgaacg agcgtggcgg cgagatcgat ctgataatgc gtgaaggcct gaccaccgtt
     5041 tttgtcgagg tacgctatcg ccgctctgcg ctttatggcg gcgcggcagc cagtgtgacc
     5101 cgcagcaaac aacacaaatt attacagact gcccgcttgt ggctcgcgcg tcataatggg
     5161 agttttgata ctgtggattg ccggttcgat gtggtagcct tcaccgggaa tgaggttgag
     5221 tggattaagg atgcctttaa tgaccactca taattaaggt ttaaggatta gcgtgcaaga
     5281 aagaattaaa gcttgcttca ctgaaagcat tcaaactcaa attgcggcgg cagaggcgct
     5341 tccggatgcc atctcccgtg cagccatgac gctggttcag tctctgctca atggcaacaa
     5401 aatcctctgt tgtggtaatg gaacttccgc tgccaatgca cagcattttg ctgccagcat
     5461 gatcaaccgt ttcgaaacgg agcggcccag cttacctgcc attgcactaa atactgataa
     5521 tgttgtctta acggcgattg ccaacgatcg cttacatgat gaagtgtatg caaaacaggt
     5581 gcgggcgctg ggtcatgcgg gagatgtatt gttagccatt tccacccgtg gcaacagccg
     5641 cgatattgtt aaagcagttg aagccgccgt tacgcgtgat atgaccattg tggcattgac
     5701 cggctatgac ggcggcgaac ttgcaggttt gttagggcca caggatgtgg agatccgcat
     5761 tccttcgcat cgtagtgctc gcattcagga aatgcatatg ctgacggtaa attgcctgtg
     5821 cgatctgatc gataacacgc ttttccctca ccaggatgat taaggagaat acatgaaggc
     5881 attatcgcca atcgcagtcc ttatttccgc gctgctgttg caaggttgtg ttgccgctgc
     5941 cgtagtgggt accgctgctg tgggtaccaa agccgcaact gacccacgca gtgtcggcac
     6001 ccaggtggac gatggtaccc tggaagtgcg cgtgaacagc gcattgtcga aagacgaaca
     6061 gattaagaaa gaaacccgta ttaatgtcac ggcctatcag ggcaaagtgc tgctggttgg
     6121 gcagtcacca aatgctgaac tttcggctcg cgccaaacag attgctatgg gcgtagacgg
     6181 tgccaacgaa gtgtataacg agattcgtca gggccagccg attggtctgg gcgaagcatc
     6241 taacgatacg tggatcacca ccaaagtgcg ttcgcagctc ttaaccagcg acctggtgaa
     6301 atcgtccaac gtgaaagtga ccaccgaaaa cggtgaagtg ttcctgatgg ggctggtgac
     6361 tgaacgtgaa gcgaaagcgg cggcagatat tgccagccgg gtgagcggcg taaagcgggt
     6421 taccacagcg tttacgttta ttaaatagca gccccttgta atgcctgatg cgacgcttgc
     6481 cgcgtcttat caggcctaca acgatgcaaa ccgtaggtcg gataaggcgt ttacgccgca
     6541 tccgacatgt tttccctcaa atcagaacaa cagcgccagc ccgccgacaa tcacaccaga
     6601 cactgccacc atcgcccctg tcagccataa ggctttcgcc gggaacgctt tgcgcagcat
     6661 aatcagtgac ggcaaactca ctgccgggag cgtcatcaac aatgccagcg ccggagcggt
     6721 tcccatacct gccagcatca tcgtttgtac aatcggaatt tctgctgccg tgggaatgac
     6781 aaacaagcat cctgctaccg ccatcgccac cacccacatc aggctgttat cgacagcacc
     6841 atcggcatgg gggaataacc agacgcgtgc ggcacccaac accagtactg caaggatgta
     6901 aaccgggatc gtactccaga aaagtgtcca tagcgccctg ccccagcggc taaaaaaacc
     6961 gctctgtgct tccggtatgt caatttcgac cggtgcctgc gtttgcggtg tttcacgcac
     7021 ccatttttgc accagcgtcg caatcagcaa caccatcacc agcccggcca ccagacgaat
     7081 cgccgcaaaa ccccagccga ggacaaaccc cataaacacc agcgtcgccg ggtttaacac
     7141 cggattgccc atccagaatg ccagcgcacc gcccatcgat acctgttggc gacgcattcc
     7201 cgccgcgacc ggagccgcac agcaggtaca catcatgccc ggcagcgaaa acagcgttcc
     7261 cagcagcgtg ccgcgaaagc gcgattgccc aagcgtacgc aacaaccagt cacgcgggat
     7321 cagcacctga atcaacgaac cgaggatcac ccccagcacc gccgctttcc atactgcgag
     7381 gaaatagatc atcgcgtaat ccaacgccgc ttgccacgga ttagcatccg cctgcgcaag
     7441 gatagattta ccgatactgt gggtttcggc tgcagtaaac gctttgccgt agtaaggttg
     7501 ccatttcaca taccagagac cggcaatgac aacgagaaag aaaagcgcgg gtttccacca
     7561 ctgaatgggc gttgccgcct gagatgaaga ctgaccagtc atagcattcc ccaggaataa
     7621 gttatgataa ttgagcgcgt gaatattacg ctcactatca attcttggga ataattattc
     7681 agctcttttg cgtaattctg aagagcttaa aatcgtcacg ccttcatgct caggccgcat
     7741 cgcctctgcc agcatgaccc gcgccacgtc tctcgcatca atggatttcc agttacctgg
     7801 taacaaacgg aacagcggcg caaaaagcgt ttcgttcatc cgctgtttgc tacgatcgcc
     7861 cagtaacatc gacgggcgag caatggtcaa tttcggccag ttctgggcaa ttaatgcttc
     7921 ttccatctcc cctttgacgc ggttatagaa aaacggcgag tgggcattgg cacccatagc
     7981 actgaccacc agcatatgct gcgcgcccag tcgccgcccg gttaatgcgg tatccactac
     8041 cagcgtgtaa tcggcatgaa taaacgcctc tttgctcccc gcttctcgcc gcgtggtgcc
     8101 gagacaacaa aacacaatgt cgatgggttc ggtgacctgc gccaacgcgt cactcagttg
     8161 cggatcatgg ggattaaaca ccccgggcat atcgcccaac ggacgtcgcg tcggcgcagc
     8221 aatggcgtta actttcggtt cattaatcaa catccgcagc aggtgaccgc ccaccagccc
     8281 cgttgcgcct gtaatcagta cctgactcat cttcgttcct ttacagaatt gtctgccttg
     8341 cgctccccgg ctcatgcacc agtcttaata gaccatgagg taattatccc cgattgtgga
     8401 aaattcgcct catccaatgc aacaacgcgg aggaagcatg agtaagaaaa ttgccgtttt
     8461 aatcactgat gaatttgaag attcagaatt tacttcaccc gcagacgagt tccgtaaagc
     8521 cggacacgaa gtgattacta ttgaaaaaca agcgggtaaa acggtgaaag gcaaaaaagg
     8581 agaagccagc gtgaccatcg ataaatccat cgatgaagtg acgccagcgg agtttgatgc
     8641 cctgttgcta ccgggtggtc attcaccgga ttatctgcgt ggcgacaacc gttttgtcac
     8701 ctttacccgt gattttgtga atagcggcaa acctgttttc gccatctgtc acggtccgca
     8761 gctgctgatc agcgccgatg tgattcgggg gcgcaaactg accgcagtta aaccgatcat
     8821 tattgatgtt aaaaatgcgg gcgtggaatt ttacgatcag gaagtcgtgg ttgataaaga
     8881 tcagctggtt accagccgga caccggacga tctgccagcg tttaaccgcg aggcgttacg
     8941 cctgctcggt gcctgagtcg cgtagccaaa tcattttttt accaaagccc agcgtgttgt
     9001 cggtgaattt gatttcatcg agccggattt cccacaccgg ggccgacagc attctggcaa
     9061 ccggaaagcg acgattgtac gccttgcgcg cgaggtcgct ttcctcacct tccagcctgc
     9121 ggatctcacc tttaaactgc acaccgcgaa ttaacgctac cgttttcggc tgaccgttta
     9181 ccgttccggc aaccgcagcc tgcggcccac tcatctgcgc gtggcgcgtt ttttcttccg
     9241 tcaaaatgta gaaggcgact ttctgcgcat caaaaagata aaaggcatta gcgcaccaga
     9301 gttccccttc ctgctgcaca caccaggtga caacatgttg ttttgccagc caacggctga
     9361 tggcgatgag tgtttccatt gctgttctct tttatactgt gggcctgacc ttaacatgcg
     9421 atgcagacga tgacaccctg gtttctttac ttgatccgta ccgccgacaa taagctttat
     9481 accgggatca ccacggatgt cgaacgccgc tatcagcagc accaaagcgg caaaggggcg
     9541 aaagcactgc gcgggaaagg agaactgacg ctggcgtttt ctgcgccagt gggcgaccgt
     9601 tcgctggcac tacgggcgga atatcgcgtt aagcaactga ccaaacggca aaaagagcgt
     9661 ctggtagcgg aaggcgcagg gtttgcagaa ctgttgagca gcctgcaaac cccggagatt
     9721 aaaagcgatt gaaatgctcg tgatactcaa ccagaccggt tacgccattc agcgcgtcat
     9781 ccgctaaacg atgtaccagg aaggcgcttt cagtgcccgg ccagcggcaa cgcagatcgt
     9841 gatgcgccgc cagttcaaag ccgaaacgac tgtacagcgc cggatcgccc agcgtcacca
     9901 ctgcggcata gccgaactca ttaagcgaat cgagtccttc atagaccagt tggcgtgcca
     9961 gcccctgtcc acggtatttt tcatcaaccg ccaacggtgc catgccgacc cattgcaggt
    10021 cttcgccctg cacatcaacc ggactaaatg ccacatagcc aatgacctga ccttcgtcat
    10081 ctgtcgccac cagccccagc gtcagaaagc catcttcacg cagatcgtga accagcttcg
    10141 cttccgcatc actttcgaat gagcgacgca gcagggcatc aatacccggc gcatcaatgg
    10201 gaatttctac tcgaattagc atggttcacc taccgatgtc tgtttggttt caggcgcggt
    10261 tttcattccc gcctcaacaa aatccgccag ttgcagcagc atcatgcgta aggctttcgg
    10321 catttgctcc agttcaatgg cgtccatcag gtttttcaca tacaccccca gctccgtatc
    10381 gccttcaatc accagccgac gctggaagaa gagcgtatcc ggatcttgtt tacgcgccgc
    10441 aatcatcagc agatcgctgg cgtcggcact aaaactcaca tcagcttgcg tgttctggct
    10501 gaccaccagt ttgccattca ccaccgaggt aaaccattgc aggtcaatat cacgcacatg
    10561 aatacttaac cagcggcctt caagaaactc cagctcgcca tcatccagcg cctgacggaa
    10621 ttgccagctt aagacctgct caagaacctg gcgttttagc gcaaatggcg tcagttttac
    10681 cggtacactc aacagagatg gccccaaatg cacaatacgg gaacgcagtt tatccaacac
    10741 gagttttact ccctgtttca acaatcatcc tattttgcca tatcagaaaa ataacatagc
    10801 ggtataaatc aacaattcca tatgaaattg ctgctaccac caatacaact ttaactgcct
    10861 taaatcaaaa attgtcgcag caaggttaac taaaatccca gttcgttaac atttttgcgt
    10921 tttgatagcg caaccttcag gaaaaattat ggagctgctc tgccctgccg gaaatctccc
    10981 ggcgcttaag gcggccatcg aaaacggcgc agatgctgtt tatatcgggc taaaagatga
    11041 taccaatgcc cgtcacttcg ccggccttaa ctttaccgag aaaaaattgc aggaagcggt
    11101 gagttttgtc catcaacatc gccgcaaact tcacatcgcg attaacactt ttgcgcatcc
    11161 ggacggttac gcccgttggc agcgtgccgt ggatatggcg gcgcagctgg gtgccgacgc
    11221 gctgatcctc gccgacctcg ccatgctgga gtacgccgcc gagcgttacc cgcatattga
    11281 acgtcatgtg tcagtgcagg cttcggcgac caatgaagag gcgattaact tttatcatcg
    11341 ccattttgac gttgcccgcg tggtgctgcc gcgcgtgttg tcgattcatc aggtgaaaca
    11401 gctggcacgg gttactcctg tcccgctgga agtgtttgct ttcggcagcc tgtgtattat
    11461 gtcggaaggc cgttgctatc tgtcgtcgta tctgacgggt gagtcgccca acactgtggg
    11521 tgcgtgttct ccggcccgtt tcgtgcgctg gcaacaaacg ccgcaggggc tggaatcccg
    11581 cctgaacgaa gtgctgatcg accgttatca ggacggcgaa aacgcaggtt atccgacgct
    11641 gtgtaaaggg cgttatctgg tggacggcga gcgctatcac gcgctggaag aaccaaccag
    11701 tctcaatacc ctggaactgc tgccggagtt aatggctgcg aatattgctt cggtgaaaat
    11761 tgaaggccgc cagcgtagcc cggcgtatgt cagccaggcg gcgaaagtct ggcgtcaggc
    11821 tatcgaccgt tgtaaggccg atccgcaaaa cttcgtaccg caaagcgcgt ggatggagac
    11881 gctcgggtcg atgtccgaag gcacgcaaac cacccttggc gcgtatcacc gtaaatggca
    11941 gtgagaagag caatgaaata ttccttaggg ccagtgctgt ggtactggcc aaaagagacg
    12001 ctggaagatt tttatcagca agccgccgcc agcagcgccg acgtgattta tcttggtgaa
    12061 gcggtatgca gcaagcgccg ggcaaccaaa gttggcgact gtctggagat ggcaaaatcg
    12121 ctcgccggaa gtggcaagca gattgtgctc tccacgctgg cgctggtgca ggcatcctct
    12181 gaactgggcg aactgaaacg ctatgttgag aacggtgaat ttctgattga agccagcgat
    12241 ctcggcgtgg tgaatatgtg cgctgaacgc aaactgccgt tcgtcgccgg gcacgcgctg
    12301 aactgctaca acgcggtgac gctgaaaata ttgctcaaac agggcatgat gcgctggtgt
    12361 atgccggtgg agctttcccg cgactggctg gtgaatctgc ttaatcagtg cgatgagctg
    12421 ggcattcgca accagtttga agtggaagtc ctgagctacg gtcatctacc gctggcctac
    12481 tccgcccgct gctttaccgc gcgttcggaa gaccgcccga aagatgagtg tgaaacctgc
    12541 tgcattaagt atccgaacgg gcgcaacgtg ctgtcgcagg aaaaccaaca agtgtttgta
    12601 ctcaatggca ttcagaccat gagcggctac gtttacaacc tcggtaacga gctggcatcc
    12661 atgcagggat tggttgatgt agtccgcctg tcaccgcagg gcactgacac tttcgcgatg
    12721 ctcgacgcct tccgcgctaa tgaaaatggc gctgtgccac tgccgttgac ggcaaacagc
    12781 gactgtaacg gctactggcg gcggctggca ggactggagc tgcaagccta agtaaatagc
    12841 tcactttgtt aacaacttta actattcttt aatgcagtat taaagattaa tcggtaacaa
    12901 agtgagctgt tatgactgat aaaaccattg cgttttcgct actcgatctg gcccccatac
    12961 ccgaaggctc ttcagcgcgg gaagcgttct cccactctct cgatctcgcc cgtctggctg
    13021 aaaagcgcgg ctatcatcgc tactggctgg cagaacacca caatatgacc ggcattgcca
    13081 gtgctgcaac atcggtattg attggctatc tggcggcgaa taccaccacg ctgcatctgg
    13141 ggtctggcgg cgtgatgttg cctaaccact taccgttggt cattgccgaa cagttcggca
    13201 cactcaatac gctctatccg gggcgaatcg atttggggct gggtcgtgct ccgggtagtg
    13261 accaacggac aatgatggcg ctacgtcgtc atatgagcgg cgatattgat aatttccccc
    13321 gcgatgtcgc ggagctggtg gactggtttg acgcccgcga tcccaatccg catgtgcgcc
    13381 cggtaccagg ctatggcgag aaaatccccg tgtggttgtt aggctccagc ctttacagtg
    13441 cacaactggc ggcgcagctt ggtctgccat ttgcgtttgc ctcacacttc gcgccggata
    13501 tgttgttcca ggcgctgcat ctttatcgca gtaacttcaa accgtcggct cggctggaaa
    13561 aaccatacgc gatggtgtgc atcaatatta tcgccgccga cagcaaccgc gacgctgaat
    13621 ttctgtttac ctcaatgcag caagcctttg tgaagctgcg ccgtggcgaa accgggcaac
    13681 tgccgccgcc gattcaaaat atggatcagt tctggtcacc gtctgagcag tatgacgtgc
    13741 agcaggcgct gagtatgtcg ttggtaggcg ataaagcgaa agtgcgtcat ggtttgcagt
    13801 cgatcctgcg cgaaaccgac gctgatgaga ttatggttaa cgggcagatt ttcgaccacc
    13861 aggcgcgcct gcattcgttt gagctggcga tggatgttaa ggaagagttg ttgggatagt
    13921 gtgtcttaac gcgggaagcc ttatccgagc tggcaacgct gtcctacgta ggcctgataa
    13981 gcgaagcgca tcaggcattg tgtaggcagc agaaatgtcg gataaggcac cgctgattac
    14041 tgatacaccg gcagtaaatt aaagctcgat aaaatatgca ccagtgcgtt gccgacgcca
    14101 aacaccagaa tcagcgcaat catcggcttg ccaccccaga cgcggaattt cgggctgcca
    14161 aagcgtttac gcgatgcgcg ggccagcagt gccggaacaa ttgccgccca gatggtagcc
    14221 gctaaaccag cataaccaat ggcatacagg aatccgttcg ggaacagcag accacccaca
    14281 acaggcgggg caaaggtcag caatgccgtt ttcaagcggc ccacagccga gtcgtcgaaa
    14341 ccaaacagat ctgccagata gtcaaacaaa cccagcgtta cgccaaggaa cgaactcgct
    14401 accgcaaagt ttgagaacac gaccagcaga aggtccaggc tacggctgtt cagcacgccg
    14461 cttaatgctt gtaccaacac atcaatatta ccgcccttct cggcgatgcc gataaactcc
    14521 ggacgaggga tgttgcccat tgtcgccagc aaccagatgg tatacagcgc cagtgccatc
    14581 agcgtaccgt acaccaggca tttcacgatg gttttcggat ctttgccgta atacttcatc
    14641 aggcttggca cgttaccgtg ataaccaaac gacgccaggc agaacggcag ggtcatcagc
    14701 aaatacggcg cataagatgc attgctttcg gcaacgttga acaatgtcgc aggctgcaca
    14761 tgccccagca ggctaccgaa ggtgaggaag aaggtaatga ctttcgcccc cagcacaatc
    14821 gccgtcatgc ggctgacggc tttagtactc aaccacacca caaacgctac cagcagtgca
    14881 aaaccaaaac ccgccgcccg tgccgggacg tttagcgaca tctctgcgaa ggtgtgatgc
    14941 agaatcgaac cactggcaga aatataggca taggtcagga tatagagcac aaaggcaatg
    15001 gaaatgccgt tgaccacgtt ccagcctttg cccagcaaat ctttggtgat ggtgtcaaaa
    15061 ctcgaaccga ttcggtaatt caggttagct tccagaatca tcaagccgga atgcagcata
    15121 cagaaccagg taaagatcag cgctgccatt gaccagaaaa accacgcccc ggacatgacc
    15181 actggcagag aaaacatccc tgcgccaata atggtgccgc cgataatcac cacgccgcca
    15241 agcagcgacg gtgacgtttg ggtggtggtt agtgttgcca tgagggcttc tctccagtga
    15301 aaaatagtgc gactgcgttg ttatgcattg cactgtacca gtacatgagt acaaaagaca
    15361 gaaaaaaagc cccgatggtt aaaatcgggg ctgtatatat tattttacag attgtgttcg
    15421 ctgttcagcg atgattacgc atcaccaccg aaacgacgac gaccggtaga gtcatcacga
    15481 cgcggtgcgc ggccttcacg acgttcgccg ctaaaacgac gaccatcacc acgaccacct
    15541 tcacggcgtt caccgctgaa gttacgaccg ccttcacgac gctcgccacc gaaaccacga
    15601 ccaccgccac gacgctcacc gccagtatgc ggctgtgcat cgcccagtaa ctgcatgttc
    15661 atcggcttgt tgagaatgcg agtgcgcgta aagtgttgca gcacttcacc cggcatacct
    15721 ttcggcagtt cgatggtgga gtgagaagca aacagcttga tgttaccgat gtaacggctg
    15781 ctgatgtcgc cttcgttagc aatcgcgcca acgatatgac gaacttcaac accatcatcg
    15841 cggcccactt caatgcggta cagctgcata tcgccaacat cacgacgttc acgacgcgga
    15901 cgatcttcac ggtcgccacg cgggccacgg tcgttacgat cgcgcggacc acggtcatca
    15961 cggtcacgga attcacgttt cggacgcatc ggcgcatctg gcggaacaat cagagtacgt
    16021 tcaccctgtg ccattttcag cagtgccgca gccagagttt cgagatccag ctcttcacct
    16081 tctgcagtcg gctgaatttt gctcagcagt gcgcggtatt gatccagatc gctgctttcc
    16141 agctgctgct gtactttagc ggcgaatttt tccagacggc gtttgcccag cagttctgcg
    16201 ttcggcagtt ctacttccgg aatagtcagc ttcatagtac gttcaatgtt gcgcagcaga
    16261 cgacgctcgc ggttctcaac gaacagcagc gcgcggccag cacgacccgc acgaccggta
    16321 cgaccgatac ggtgaacgta agactcagaa tccatcggga tatcgtagtt aaccaccagg
    16381 ctgatacgct caacgtccag gccacgggcc gcaacgtcgg tcgcaatcag gatgtccaga
    16441 cgaccgtctt taaggcgttc cagtgtctgc tcacgcagcg cctggttcat gtcaccgttc
    16501 agcgcggcgc tattgtagcc gttacgctca agagcttcag ccacttccag agtcgcgttt
    16561 ttggtacgaa cgaagataat cgccgcatca aaatcttccg cttccaggaa acgtaccagt
    16621 gcttcgtttt tacgcatacc ccacacagtc cagtagctct ggctgatgtc aggacgggta
    16681 gtcacgctgg actgaatacg cacttcctgc ggctctttca taaagcggcg ggtaatgcga
    16741 cgaatcgctt ccggcatggt tgcagagaac agagcggtct gatgaccttc cgggatctgc
    16801 gccataatgg tttcaacgtc ttcgatgaag cccatgcgca gcatttcgtc agcttcatcc
    16861 agaaccagac cgctcagttt agagaggtcc agagtgccac gtttcaggtg gtccagcaga
    16921 cggcccggag taccgacaac gatctgcggc ccctgacgca gggcgcgtaa ttgcacgtca
    16981 taacgctggc cgccgtacag ggcaaccaca tttacgccgc gcatgtgttt agagaaatcc
    17041 gtcattgctt cagcaacctg taccgccagt tcgcgggtcg gtgccagcac cagaatctgt
    17101 ggtgctttca gctcaggatc aagattctgc agcagaggta aagagaatgc tgcagttttt
    17161 ccgctccccg tctgggccat acccagaacg tcgcggccat tcagcagatg tggaatacac
    17221 tctgcctgaa ttggagatgg tttttcgtaa cccagatcgt taagggcttc aaggatagga
    17281 gccttcaggc ccagatctgc aaaagtggtt tcgaattcag ccatgtagta cgtgtgcctc
    17341 aaaattaatg gcggccagtc tacataactc atcatgaaat tgatcagcaa ttttcattga
    17401 aaagtgtgaa ccggctcaaa gtaggtgtat taacgaacaa caacgccctc acccgttaag
    17461 gtgatggcaa tcaaaaaaga ttacgggctg atgtgtacgt cagctattgc tggtccgatt
    17521 ctgccaggtc atcttggtcc tggcccagga gcgataattc caacaatgcg tatcggtgct
    17581 caacaaagtt atgaacgttg ttggcaaccg ccagtttgaa cagtgccgtg gcgctgtcca
    17641 aatcccccag acttaggtag tacttaccta aatagaagtt ggtttcactg agatgctcag
    17701 cgagcgaggt gttatccgtt gcgtccgcct tgagcctttc cattaacgtt tgttcgctaa
    17761 tgttgcccag gtagaactcg acaatgttcc atccccactg ttccttatcc gatttttcga
    17821 agtgctgttt caatacttct ttagcctgct tctcatcgag cttctgctcg gcgagataaa
    17881 gccacagact acggaaagga tcattgggat cgtcttgata aaacgccagc agatcatctt
    17941 gcgctaactt gtcacgaccg ccgtaatata atgcaatccc gcgattcaag tgcgcgtagt
    18001 tgtaagttgg atcaagctca agtacagaat caaacgcttc ataggcagca tcaaaattgc
    18061 ctgcctgcgt taaatatatg cctaagtaat tgaatacttc aggcatatcc ggtcggattg
    18121 ccagcgcttg cgaaaaatcg ttacgcgcta atgccctcag accgagacta tcatacaaca
    18181 ctccgcgctc atataaaagc tgtgcgcgtt cgtcatcggt taaagcccga ctggcaagga
    18241 tttgttccat acgtgccaga atcacttcct gctgtaaagt cggttgcaat ggtaccgcga
    18301 ggacttcact tttacgccag gaagtattac tgcatcctgc aagcgtaagt gctgtcgcaa
    18361 cgaaacacca gcgcaaaaaa ggcttcattt cccactcccg aagaccacgg ttgaatgaac
    18421 gtcctgttcc cggttgctaa caaggcgtcc tgcccggtta aaagcccccc gccgcagcgg
    18481 agggcaaatg gcaaccttac tcgccctgtt cagcagccgg agcttccggt gctgcagcag
    18541 gttgagactg ctcagtcgct tctttaatgc tcagacggat acggccctgg cgatcaactt
    18601 ccagaacttt caccggtact tcctgaccca tctgcaggta atcggtcact ttctcaacgc
    18661 gtttgtcagc gatttgagag atgtgtacca gaccttcttt accgccgccg atggcaacaa
    18721 atgcgccaaa gtcaacgata cgggtcactt taccagtgta gacgcggccc acttcgattt
    18781 ctgcagtgat ctcttcgata cgacgaatag catgtttcgc tttctcgccg tcggtcgctg
    18841 caatcttcac agtaccgtca tcttcgattt cgatggtggt gccagtttct tcggtcaggg
    18901 cacggataac agagccgcct ttaccgataa cgtctttgat cttgtccggg ttgatcttga
    18961 tggtatggat acgtggtgcg aactcagaga tatcgccacg cggcgcgttg atcgcctgtt
    19021 ccattacgcc caggatatgc agacgcgcac ctttagcctg gttcagcgca acctgcatga
    19081 tctctttggt gataccttca attttgatat ccatctgcaa tgcagagata ccctcgcggg
    19141 aacccgcaac tttgaagtcc atatcgccca ggtgatcttc gtcgcccaaa atgtcagaca
    19201 gtacaacgta gttgtcgcct tctttcacca gacccattgc gatacccgca acggcagctt
    19261 tgatcggcac acctgcgtcc atcagcgcca gagacgcgcc gcacacggaa gccatagaag
    19321 aggaaccgtt ggattcagtg atttcagaca caacacgtac ggtgtacggg aatttgtcca
    19381 tatccggcat gactgccagc acgccgcgct tcgccagacg accgtgacca atttcacgac
    19441 gcttcggaga accgaccatg ccggtttcgc ctacggagta cggagggaag ttgtagtgga
    19501 acaggaaggt atcggtacgt tcgcccatca gttcatcaag aacctgcgcg tcacgagcag
    19561 tacccagcgt tgcggtaacc agcgcctgcg tttcaccacg ggtgaacagc gcagaaccgt
    19621 gagtacgcgg cagcacgcca gtacgcacat ccagaccacg gatcatatct ttttcacgac
    19681 cgtcgatacg cggttcgcct gccagtacgc ggctacgaac aacgtttttc tcgatcgcgt
    19741 gcagaatttc acccagttcg ttttcgtcca gggtttcgtc ttcagcaagc agcgtcgcga
    19801 tggtttcaga tttgatgaca tcaacctgcg cataacgctc ttgtttgtcg gtgatgcggt
    19861 aagcatcgct caggcgagct tcagccagtg cagcaacgcg cgcgttcagc gcttcgttta
    19921 ccggctccgg ctgccagtcc caacgtggtt taccggcttc tttcaccagt tcattgatgt
    19981 tctgaataac aacctgctgt tgttcatgac cgaacactac tgcgcccagc atctggtctt
    20041 cgctcagcag ttcagcttca gattcaacca tcagtacagc ggcttcagta ccggcaacaa
    20101 ccagatccag tttgctctct ttcaactcgt cctgagtcgg gttcagtacg tactggtcat
    20161 tgatgtaacc tacgcgagca gcaccaatcg gaccattgaa cggaatacca gacagagaca
    20221 gtgctgcgga agcaccaatc atcgcgacga tatccgggtt aacttgcggg ttaacagaaa
    20281 ccacggtggc gataacctga acttcgttga cgaagccttc cgggaacagc ggccgaatcg
    20341 ggcggtcaat cagacgcgcg atcagggttt cgccttcgct tgggcggcct tcacgacgga
    20401 agaagctacc cgggatacga ccagcagcgt aggtacgctc ctgatagtta acggtcagtg
    20461 ggaagaagtc ctgacctggt ttggcttttt tctggccaac aacggtaaca aataccgcgg
    20521 tgtcatccat gctaaccata acagcggcag tagcctgacg agccatcatg ccggtttcca
    20581 gagtcacggt gtgttggccg tactggaatt tacgaacgat cggattaagc aatgtaatat
    20641 cctttctctt tcttagacag taccttacgg cactggtgtt aatacccgat cttctgcgca
    20701 tcctcgcgac taatgacaac cctaacccag ctctatgtgg gtaaagcctc tcattagccg
    20761 cgcgaacctc tgcaacggaa gatcattcat agcaacaata cattagtttc cagtgaattg
    20821 ctgccgtcag cttgaaaaaa ggggccactc aggccccctt ttctgaaact cgcaagaatt
    20881 agcgacgcag acccaggcgc tcgatgagct gggtgtaacg tgctacgtct ttacgtttca
    20941 ggtagtcgag cagtttacga cgctgagaaa ccatgcgcag cagaccacga cggctgtggt
    21001 gatctttttt gtgctctgca aagtggccct gcaggtggtt gatctgtgca gtcagcagtg
    21061 ctacctgaac ttcagtagaa ccggtgtcgt ttgcgtcacg accaaactca gaaacgattt
    21121 tagctgttgc ttcagtactt agagacattt taaaactcca aagtatatag aatgaaagga
    21181 cgccgatctc taattcagcg atcccagtgt acgttacgca aagtgttaaa caatttacgc
    21241 gacgttaagc ggcagtattc tactcgtagc gacctgttat cgcaagacgg ttaacattac
    21301 gccgggtatt caaccaccag gcgacgaggc gcaacgcggc cttcatcgtc aatttcgccc
    21361 ataccgataa atttgccgtt ctcaccttct gtaacgcgaa ccagtccttc cagcggcgca
    21421 ccggatgtac gaactgggtt accatttttg aagtaaacag aagacgttaa cggaagattc
    21481 accaccgggt agtccgaagc tggactgtcc attggcatca gtaatggatc aagtaactcc
    21541 gcagccggaa tatcctgctg ttcggcttgt tcgaccagtt cacgcagatg ttccagggtc
    21601 acaatccgtt caaccggata tttacttacc gccagacgac gcaggtaaat aacatgcgcg
    21661 ccacaaccga gtttttcacc caggtcatca atgatggtgc ggatataagt gccttttgag
    21721 cagtgaattt ccagctccag ctcattgcct tcgtggcgaa taaacagcaa ttcataaacg
    21781 gtaatcgggc gcgcttcacg cggtacttca atgccctgac gcgcatattc gtacagtttt
    21841 ttaccctgat atttcagtgc tgaatacatc gaagggatct gttcgatatc gccacggaaa
    21901 gtatccagcg ccgccgccag ttgctctgcg ctaaaggtta ccggacgttc ttcaacgatt
    21961 tgtccgtcgg catcagaagt atcggtacgc tgtccaagac gcgcaatgac ccgatagcgt
    22021 ttgtctgagt ccagcagata ctgggaaaac ttcgtcgctt ccccgaggca aattggcaac
    22081 atgccagttg ccagcgggtc cagcgcaccg gtatgcccgg cacggttggc gttataaatc
    22141 cgtttcactt tttgcagcgc atcattgctg gacataccct gcggtttatc cagcaacaaa
    22201 acgccgttaa tgtcgcgacc gcgacgacga ggacgactca ttagtcctcc ttgctgtcgt
    22261 ccgggttaac acgacgttct tcgtcatgtt tgaccacgct ggtcaccagg tttgacatgc
    22321 gcatcccttc aaccagagag ttgtcgtaga agaaggtcag ttccggcacg atacgcaggc
    22381 gcatcgcttt ccccagcagg ctgcggatga aaccagaagc ttcttgcaac gctttgatgc
    22441 ccgctttaac cgcgtcttca tctttgtcgt tgaggaacgt cacatatact ttggcatacg
    22501 ccaggtcgcg agacatttcg acaccggaaa cggtggtcat catgcccagg cgaggatctt
    22561 taatttcacg ctgcaggatg agagcgatct ctttttgcat ttcctgcgct acgcgctgcg
    22621 ggcgaccaaa ttctttcgcc ataataaatt ctcctgacaa aaaaggggct gttagcccct
    22681 ttttaaaatt aatttcaggt ggaagggctg ttcacgttgg cctgataaga cgcgccagcg
    22741 tcacatcagg caatccatgc cggatgcggc gtaaacgcct tatcccgcat ggaaccctaa
    22801 aaaccttaag cgatggtacg ttggatctcg atgatttcga atacttcgat cacatcgcca
    22861 gtgcggacgt cgttgtagtt cttaacgccg ataccacatt ccataccgtt acggacttcg
    22921 ttaacgtcat ctttgaagcg gcgcagggac tccagctcgc cttcgtagat aaccacgttg
    22981 tcacgcagaa cgcggatcgg gttgtgacgt ttaaccacac cttcggtaac catacagcct
    23041 gcgatggcac caaatttcgg cgatttgaac acgtcacgaa cttccgccag accgataatc
    23101 tgctgtttca gttccggaga cagcataccg ctcatcgccg ctttcacttc gtcaatcagg
    23161 ttatagatga cggagtagta acgcagatcc aggctttccg cttcaatcac tttacgtgca
    23221 gaggcatcag cacgtacgtt gaagccaacc aggatggcgt tggacgccgc agccagggtg
    23281 gcgtcggttt cggtgatacc acctacgcca gaaccgatga tcttcacttt aacttcgtca
    23341 gtagacagtt tcagcaagga gtcggagatc gcttccacag aaccctgtac gtctgccttc
    23401 aggacgatgt tcacttcgtg aacttcgcct tcggtcatgt tggcgaacat gttctcgagt
    23461 ttagatttct gctgacgcgc cagtttaact tcgcggaatt taccctgacg atacagtgca
    23521 acttcacgcg ctttcttctc gtcacgtaca acggtaactt catcacccgc agccggtacg
    23581 ccggacaggc cgaggatttc caccggaatg gacggacccg cttccagcac ttcctgaccc
    23641 agttcgttac gcatcgcacg aacacgaccg tattcgaagc cacacagaac gatatcgccc
    23701 ttgtgcagag taccttcacg taccagaacg gtagcaaccg gaccacgacc tttatcgagg
    23761 aaggattcga taaccgcacc gctcgccata cctttacgta ccgctttcag ctccagaact
    23821 tccgcctgca gcaggatagc gtccagcagt tcatcgatac cagtacccgc tttcgcagac
    23881 acatgtacga actggctttc accgccccac tcttccggca ggatgccgta ctgggagagt
    23941 tcgttcttaa cgcgatccgg atcggcttct ggtttatcga tcttgttcac tgcaacgacc
    24001 accggaacct gcgccgcttt cgcgtgctgg attgcttcga tcgtctgcgg cattacgccg
    24061 tcgtcggcag caacaaccag gactacgatg tccgttgcct gcgcaccacg agcacgcatt
    24121 gaagtaaacg cggcgtgccc cggggtgtcc aggaaggtga tcatgccgtt ttcagtttca
    24181 acgtggtatg caccaatgtg ctgggtaatg ccgcccgctt cgccagaggc cactttcgtt
    24241 gaacgaatgt agtccagcag agaggtttta ccgtggtcaa cgtgacccat gatggtcaca
    24301 accggcgcgc gcggttcagc cgcagcaccc gtgtcacggt cgctcattac cgcctcttcc
    24361 agctcgtttt cacgacgcag gataacttta tggcccatct cttcagcaac cagctgtgcg
    24421 gtttcctgat cgataacctg gttgatggtt gccattgcgc ccagtttcat catcgctttg
    24481 atgacctgag agcctttaac cgccatcttg ttcgccagtt cgccaacggt gatagtttcg
    24541 ccgatcacaa cgtcacggtt aacggcctga gcaggcttct ggaagccttg ctgcagcgaa
    24601 gaacctttac gttttccgcc tttaccgcca cgtactgctg cgcgtgcttc ttcacgatca
    24661 gcttttgatt cagcgtgttt gttgcctttc ttcggacgcg ctgctttcgc gttacgacca
    24721 cggccacggc cgccttcgac ttcacgatcg ctttcgtctt ctgcctggcg agcatgttga
    24781 gaagtagtga cgtgataatc gctggaatct tcagtcggtt ccgcgttatc agtccatttg
    24841 ttttcttccg ccatacgacg tgcttcttca gcaacgcgac gtgcttcttc ttcgagttta
    24901 cgacgcgctt cttcttcagc tttacgcttg agctctgcag cttcctgctc acggcgggct
    24961 ttttcagcct gggcgttttt agtcatatcg tcttgttgat tgctcacttt gtctttttcc
    25021 gcagcttcac gtttcgcttg ttcagcagct tcacgcttag cttgttctgc ggcctcacgt
    25081 tcagcttttt gttgcgcctc gcgtttagcc gattcttctg cctcacgacg ggcttgctct
    25141 tccgctgcaa ggcgttcagc ctcttgcgga tcgcgtttca caaaggtgcg tttcttgcgg
    25201 acttcgattt gtaccgattt gctttttcca ccggtaccag gaatgttaag ggtgctgcgt
    25261 gttttacgtt gcagcgtcaa tttgtccggg cctgaatttt tctgattcag gtggtcaatc
    25321 aaagtctgtt tctcttgtgc agacacagag tcgtcagcag acttccggat acctgcatca
    25381 gcaaattgct gtaccaggcg ttccacggag gtctgtcgct ctgcggccag cgttttaatc
    25441 gttacatctg tcatgctgtt ccttcctgct acagtttatt acgcttcgtc accgaaccag
    25501 caaatattac gggcagccat aatcagtgct ccggcttttt cgtcggtcaa cccttcgata
    25561 tcagccagat catcaatgcc ctgttcggcg agatcttcca gcgtacaaac gccacgggcg
    25621 gccagtttga atgccaaatc acgatctacc ccttcaaggt tcagcagatc gtcagccggt
    25681 ttgttatcac cgaggctttc ttcctgggcc tgtgcaatgg tggccagtgc atttttagca
    25741 cgctcgcgca gtgcttcaac ggtcggctca tcaaggcctt cgatttccaa cagctctttc
    25801 atcggcacat aggccagttc ttccagcgtc gagaaacctt cttctaccag aacagtcgcg
    25861 aagtcttcgt cgatgtcgag atatttggtg aaggtgtcga tcgctgcgtg cgcttccgcc
    25921 tgatgcttag cctgcaggtc gtcaacggtc attacgttga gttcccaacc gctcagctgc
    25981 gaagccagac gcacgttctg accgttacgg ccaatcgcct gcgccaggtt accagcttca
    26041 acggcgatat ccatggtgtg tttatcttca tccaccacga tagaagcaac gtctgccggt
    26101 gccattgcgt taatcacaaa ctgcgccggg ttatcatccc acaggacgat atcgatacgc
    26161 tcgccgccca gttcagtaga aaccgcctga acacgcgcgc cacgcatacc tacgcaagca
    26221 cctaccggat cgatacgttt atcgttggtt ttcaccgcga ttttcgcacg agaacccggg
    26281 tcgcgagccg ctgctttaat ttcaatcact tcttcgccga tttccggcac ttcaatacgg
    26341 aacagttcga tcagcatttc cggcttggaa cgagtgacga acagttgcgc gccacgcgct
    26401 tccgggcgaa cggaataaag cacgccacga acgcggtcgc cagggcggaa gttttcacgc
    26461 ggcagcatat cttcgcgcag gatcacggct tcagcgttgt tgcccagatc cagagagatg
    26521 ttgtcgcggt ttactttttt caccacgccg gtgatgattt caccttcgtg ttcacggaac
    26581 tgatcaacca ccatcgcacg ttcggcttca cgcactttct gcacgataac ctgttttgcc
    26641 gtctgggtgg tgatacggtc aaaggtaaca gactcaatct gatcttcaac gtaatcgccc
    26701 aagttcaggc tttcatcttc ataacgtgcg gcttcaaggg tgatttcctt ggtcggctgg
    26761 gtgacttcat caacaactaa ccagcgacgg aaggtgtcaa aatcaccgct tttgcgatcg
    26821 atctgtacac ggacgtcgat ctcttgctca tattttttct ttgttgctgt cgccagcgcg
    26881 ctttccaatg cttcgaaaat cttctcgcga ggtagcgcct tttcattgga tacggcttca
    26941 actacagcca aaatttcttt gttcatcgcg ggcttttcac ctcatccaga ctattaaaag
    27001 tggggaacca ggttcgcctt ctggatatta ctcagcgcga acacttcatc tttaccttcg
    27061 acggtaactg tgatcatttc accgtctacc gctttgataa cgccctgcca tttacgacgg
    27121 ttttgtaccg ccatacggag aaccagagtc acctcttctc cgacaaaacg ggcgtagtgt
    27181 tcagccgtga acagtgggcg atcgagaccc ggtgaggaga cttccaggtt ataagcaacg
    27241 gtgatgggat cttcaacatc cagcacagca cttacctggt ggctcacatc agcacaatca
    27301 tcaacattga tgccatcttc actatcaata tagatgcgca gtgtggatgt gcgaccgcga
    27361 ataaattcga tgcctaccag ttcaaaaccc agggcctcaa ctggcgcagt aatcatctct
    27421 gttaattttt gctctaatgt ggacaagccc acccccaaga cataaaaaaa gggcctaaag
    27481 cccagttatt ctgtagtcag ataacaaaaa accccgataa atcggggctt tatataactg
    27541 aaccctataa ccgcaactgc ggtctggagc actttccaga aggatttttt caaatcccac
    27601 tacgaaggcc gaagtcttca cagtatattt gaaaaaggac tctaagggaa a
//
LOCUS       DAJTSI010000047        27464 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000047 DAJTSI010000000
VERSION     DAJTSI010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.047"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            248..1546
                     /gene="kgtP"
                     /locus_tag="OO327_001741"
     CDS             248..1546
                     /gene="kgtP"
                     /locus_tag="OO327_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR6018313.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1543..1866)
                     /locus_tag="OO327_001742"
     CDS             complement(1543..1866)
                     /locus_tag="OO327_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR6018314.1"
                     /translation="MRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1912..3267)
                     /gene="pssA"
                     /locus_tag="OO327_001743"
     CDS             complement(1912..3267)
                     /gene="pssA"
                     /locus_tag="OO327_001743"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR6018315.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3381..6041)
                     /gene="pat"
                     /locus_tag="OO327_001744"
     CDS             complement(3381..6041)
                     /gene="pat"
                     /locus_tag="OO327_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR6018316.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6073..6771)
                     /gene="tapT"
                     /locus_tag="OO327_001745"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6073..6771)
                     /gene="tapT"
                     /locus_tag="OO327_001745"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR6018317.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6840..7259)
                     /gene="trxC"
                     /locus_tag="OO327_001746"
     CDS             complement(6840..7259)
                     /gene="trxC"
                     /locus_tag="OO327_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR6018318.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7466..8503
                     /locus_tag="OO327_001747"
     CDS             7466..8503
                     /locus_tag="OO327_001747"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6018319.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8551..9240)
                     /gene="ung"
                     /locus_tag="OO327_001748"
     CDS             complement(8551..9240)
                     /gene="ung"
                     /locus_tag="OO327_001748"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR6018320.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9545..9928
                     /gene="grcA"
                     /locus_tag="OO327_001749"
     CDS             9545..9928
                     /gene="grcA"
                     /locus_tag="OO327_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR6018321.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(9984..10571)
                     /gene="eamB"
                     /locus_tag="OO327_001750"
     CDS             complement(9984..10571)
                     /gene="eamB"
                     /locus_tag="OO327_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR6018322.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10674..11555
                     /locus_tag="OO327_001751"
     CDS             10674..11555
                     /locus_tag="OO327_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6018323.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11590..12924)
                     /gene="srmB"
                     /locus_tag="OO327_001752"
     CDS             complement(11590..12924)
                     /gene="srmB"
                     /locus_tag="OO327_001752"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR6018324.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13056..13793
                     /gene="trmN"
                     /locus_tag="OO327_001753"
     CDS             13056..13793
                     /gene="trmN"
                     /locus_tag="OO327_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR6018325.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13778..15400)
                     /gene="nadB"
                     /locus_tag="OO327_001754"
                     /pseudo
     CDS             complement(13778..15400)
                     /gene="nadB"
                     /locus_tag="OO327_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15656..15811
                     /gene="rseD"
                     /locus_tag="OO327_001755"
     CDS             15656..15811
                     /gene="rseD"
                     /locus_tag="OO327_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR6018326.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15808..16383
                     /gene="rpoE"
                     /locus_tag="OO327_001756"
     CDS             15808..16383
                     /gene="rpoE"
                     /locus_tag="OO327_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR6018327.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16416..17066
                     /gene="rseA"
                     /locus_tag="OO327_001757"
     CDS             16416..17066
                     /gene="rseA"
                     /locus_tag="OO327_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR6018328.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17066..18022
                     /gene="rseB"
                     /locus_tag="OO327_001758"
     CDS             17066..18022
                     /gene="rseB"
                     /locus_tag="OO327_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR6018329.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18019..18498
                     /gene="rseC"
                     /locus_tag="OO327_001759"
     CDS             18019..18498
                     /gene="rseC"
                     /locus_tag="OO327_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR6018330.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18696..20495
                     /gene="lepA"
                     /locus_tag="OO327_001760"
     CDS             18696..20495
                     /gene="lepA"
                     /locus_tag="OO327_001760"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR6018331.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20511..21485
                     /gene="lepB"
                     /locus_tag="OO327_001761"
     CDS             20511..21485
                     /gene="lepB"
                     /locus_tag="OO327_001761"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR6018332.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21757..22437
                     /gene="rnc"
                     /locus_tag="OO327_001762"
     CDS             21757..22437
                     /gene="rnc"
                     /locus_tag="OO327_001762"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR6018333.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22434..23339
                     /gene="era"
                     /locus_tag="OO327_001763"
     CDS             22434..23339
                     /gene="era"
                     /locus_tag="OO327_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR6018334.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGSKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23351..24079
                     /gene="recO"
                     /locus_tag="OO327_001764"
     CDS             23351..24079
                     /gene="recO"
                     /locus_tag="OO327_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR6018335.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24091..24822
                     /gene="pdxJ"
                     /locus_tag="OO327_001765"
     CDS             24091..24822
                     /gene="pdxJ"
                     /locus_tag="OO327_001765"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR6018336.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24822..25202
                     /locus_tag="OO327_001766"
     CDS             24822..25202
                     /locus_tag="OO327_001766"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR6018337.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25622..25702
                     /gene="shoB"
                     /locus_tag="OO327_001767"
     CDS             25622..25702
                     /gene="shoB"
                     /locus_tag="OO327_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR6018338.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25896..26156)
                     /gene="yfhL"
                     /locus_tag="OO327_001768"
     CDS             complement(25896..26156)
                     /gene="yfhL"
                     /locus_tag="OO327_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR6018339.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26371..27464
                     /locus_tag="OO327_001769"
                     /pseudo
     CDS             26371..27464
                     /locus_tag="OO327_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6770 a         6835 c         7202 g         6657 t
ORIGIN      
        1 gcgtcttata ctcccacata tgacagattc agcaacggat gcggtttccc caacttgcct
       61 acttccatac gtgtcctcct taccagaaat ttatccttaa gctcctcaat aaccattttc
      121 ctgctaacta aattcatggt ttaggttgca taatgatacg caacaaatgt ataacatttt
      181 ctttaccaaa agaaataaac aaaagcgacc aacaaaagca tcggattacg gcaggagaca
      241 taatggcatg gctgaaagta ctgtaacggc agacagcaaa ctgacaagta gtgatactcg
      301 tcgccgcatt tgggcgattg tgggggcctc ttcaggtaat ctggtcgagt ggttcgattt
      361 ctatgtctac tcgttctgtt cactctactt cgcccacatt ttcttccctt ccgggaacac
      421 gacgactcaa ctactacaaa cagcaggtgt ttttgctgcg ggattcctga tgcgcccaat
      481 aggcggttgg ctatttggcc gcatagccga taaacatggt cgcaaaaaat cgatgctgtt
      541 atcggtgtgt atgatgtgtt tcggatcgct ggttatcgcc tgcctcccag gttatgaaac
      601 tataggtacg tgggctccgg cattattgct tctcgctcgt ttatttcagg gattatccgt
      661 tggcggagaa tatggcacca gcgccaccta tatgagtgaa gttgccgttg aagggcgcaa
      721 aggtttttac gcatcatttc agtatgtgac gttgatcggc ggacaactgc tagccctact
      781 ggttgtcgtg gttttacaac acaccatgga agacgctgca ctcagagagt ggggatggcg
      841 tattcctttc gcgttaggag ctgtgttagc tgttgtggcg ttgtggttac gtcgtcaatt
      901 agatgaaact tcgcaacaag aaacgcgcgc tttaaaagaa gctggatctc tgaaaggatt
      961 atggcgcaat cgccgtgcat tcatcatggt tctcggtttt accgctgcgg gctccctttg
     1021 tttctatacc ttcaccactt atatgcagaa gtatctggta aatactgcgg gaatgcatgc
     1081 caacgtggcg agtggcatta tgactgccgc attgtttgta ttcatgctta ttcaaccact
     1141 cattggcgcg ctgtcggata agattggtcg ccgtacctca atgttatgtt tcggttcgct
     1201 ggcagccatt tttaccgttc ctattctctc agcattgcaa aacgtttcct cgccttatgc
     1261 cgcttttggt ctggtgatgt gtgccctgct gatagtgagt ttttatacat caatcagtgg
     1321 aatactgaag gctgagatgt tcccagcaca ggttcgcgca ttaggcgttg gtctgtcata
     1381 tgcggtcgct aatgctatat ttggtggttc ggcggagtac gtagcgttgt cgctgaaatc
     1441 aataggaatg gaaacagcct tcttctggta tgtgaccttg atggccgtgg tggcgtttct
     1501 ggtttctttg atgctacatc gcaaagggaa ggggatgcgt ctttagtgac gggtcagttg
     1561 ccagacggta tagccggtgc ttgcaccggc gacatcccag gccaaatcct tccagctcca
     1621 gccgctccct tcggggcggc tatcccaaag ctcttttgac gcccccaaac tgacagagaa
     1681 catcaatcca aacatggcac tgcgatcccg gctcatcccc tgatgctgtg aatattcatt
     1741 tccggcggcg gaaagtatcg ccgaggcgat aaagtgttga gctttatcct gcccactcca
     1801 gctatcgtta tccatatggc tgcattcaga aagtaacaat agcgaacaga caaaaagaat
     1861 acgcaaaacg tggcctccaa aaaacaaacc ccgtacagga cggggttgtg attacaggat
     1921 gcggctaatt aatcggtcga tgcggatacg gcgcaaacgg cggatgagtt tacgaacctt
     1981 caccggataa tcggcaatac tttgcagatc gcgatagtgc ttaacgatgg tggtacgctc
     2041 gcggatcagc tccagttctt tctctcgctg cggcgcaagc tccagttgtg gatcgtggat
     2101 caaaatggcg ttttccagat ccagacgcca ggcgcgcggg ttcaggttat taccggtgat
     2161 caacatccac ttatcatcaa cccacatccc tttcaggtga taggtgttgt cgtcatcttt
     2221 ccataaccga accactagct ggtcagtatt gacgtaatac tgcaaacggc tcaggaaacg
     2281 acgcagattg atctcataga gataaggcaa tgcgccaatt atcttgaaag gttcatcttc
     2341 cggaatgtag aagtcgttcg cggttttatc accaacaata atttcgacct ttttcccttc
     2401 gcgcagcaac tggataatat tgcgcacaag gattgctggc aggttgaagt atggcgtaca
     2461 gatggttagt ttctgctcgg cacaaggcat aagatggaaa atggtcttgt tcaacagact
     2521 cgatttcccc agccccacta gcggcgttac ggaaagctga tcgttgtcgg catcgccctg
     2581 gaaatgataa gcggcatcac gcagctcctg gcggaacaga cgaatatcgt tcttgatttc
     2641 cgggcttttt ggccgattaa catcatccag acgattaacg ccgcggccat tcataatatt
     2701 ctgtgtaacc cattcaaaca taatgtctga catcttacgg ttacggatca gatgataacg
     2761 gtcgtagcga tatttatcgt gttgatgcag gtaaacatcg ttcaggctgg caccgctata
     2821 aagtacgcta tcgtcgatga taaagccttt aaagtgcaga acaccaagag cttcacgggt
     2881 attgattgga acgccataaa ccggaacatc tacgcccgga ttttcctgcg ccatgcggca
     2941 gtaccagtca gcgttagtgt tagatgccgc agcgccaatg cgtccacgtt gtgcacgatg
     3001 ccagtcgacc agcacccgca catccagttc cggacgctgc cttttagcct catacaacgc
     3061 gttcagaatg cctttgccac cgtcatcctg ttcgagatac agggcgacaa tgcaaatgcg
     3121 ctgcttcgcg ctggctattt tttccagcag cgtctcccgg aagtcggcgg gagcgtaaaa
     3181 gaaatcgaca tcatcaactg attgagaaat cttgggtagt tgggcaaggt gttgttgatg
     3241 tttattacgc ttaaattttg acaacatcac agtgcatttc ttctctgttc attgaagggt
     3301 cctctgtgca atgcagacga cataagcggg caataataac accagtcccg attaagtggt
     3361 caacatttcc agtaccttac tcatgattcc tcgcgccggg caagatttag cgtaagccca
     3421 acgatcccct cttcgagctg gatatcaacg ttaaacccga gcttgcgggc tagcgccacc
     3481 atgccacgat tgtttggcat cgtaatacca ttcagacgtt gtagtccgtg atttcgcgta
     3541 taggtaatca acttttccat taagcgtcga cctaagccta accctttgag atccgagcga
     3601 accagtacag caaattcggc atcgatgtta tcaggatcgg aaatcgcacg cgtgacgccg
     3661 aggatctctt ccgtttgatc aatacgtcgt accgctacaa acgccatttc ccgatcgtag
     3721 tcgatctgtg tcatgttggc taaatcttca tgggtaaatt cgttgatctc gctaaagtag
     3781 cggtaataaa gatcttcttt ggtgactcgc gaaatgaatt gctgaagttg tggctcatct
     3841 tctggcaaaa tcgggcggaa caagcagcgt tcaccgtttt tcaattctac ccattcttcc
     3901 agctgatgcg gataagggcg cactgccagc cgactctcgt tatcgccttc aaacggcgag
     3961 atatccagcg tgacatccag cgcggtaaat tcactgccag aagccagcaa aggatgaata
     4021 tccagacgct gaatttccgg gcaatcgaca atcaagttgg aaacctgcac cagaagctgg
     4081 ctcaagcctg caacatccaa tgggcgtagc gcactgcgcg cacgaatctt tttacttttg
     4141 atcccctgaa taaccagata gcgggccagg ttcatgttca gcggcggcag tgcgacgacg
     4201 gcttgatctt caggacgcca ctccacaccg ccttcaccca gcatgatcaa cggcccgaaa
     4261 accggatcgt gctcaaccac aacccgcaac tcctgagcgc cagcacggtt agccatactt
     4321 tgcaccaaca ggccgtggac ccgcgcctgt ggccaggcca tttttacgcg atcgaaaata
     4381 gcgttcgccg cttgctggac ttcattggct gtacgcaggt aaagcatgac gccctgaact
     4441 tccgatttat gtggaatatc cggcgaacgc aatttcagcg ccaccggata accaatctgt
     4501 tcggcaatat gcaccgcttc ggtgctatcg ctggcaatcc aggttgggag cgtgttcatg
     4561 ccatacgctt gcaggatggg ctgaacttca tgggtatcga gcgacgtagc cccttcggca
     4621 atcgcctgtt gcaacagaag atgcgcttct gcggtattgg aagtcagatt gctgggcaac
     4681 gccggcgttt cgcgtagttg cttctgatta cgccggtact ccaccatatg cataaaagca
     4741 gtgatggttc cttccggggt acggtaggtc ggcagcccgg cttcgctgaa taaacgtcgt
     4801 gcctcttgcg aggagtgctc gccgcaccag ttcgtcagca aagagacata tttgctgcgg
     4861 ggatgatgct ttaccgcttc aattaatact tgcgcgcttt ctgttgcggg agcagcggcg
     4921 ctgggcgaat gaataaccat cagtgcatca aaatcctggc tgtggagcag aatgtccagc
     4981 gttttgacat aatgctcgct gctggcgtca tcgcgcagat cgagcgggtt agagactgcc
     5041 acatgttctg gcaatgcatc gcgcaatttc tggcaggttt cttcgcttag cgttgccagc
     5101 ttgccattgc gtgaccataa ggcatccagc gccagcgcgg caggcgcagc accgttgctg
     5161 ataatcatca accggtcgcc acgcagcggg cgcatatggc taagggtttc caccgccgaa
     5221 aacagttcgt gggtatcctg tacccgcaac aaaccggcac gctgaatagc cgcatcccat
     5281 gccgggtcca ttcctgccgt ggtgttgagc agtcgctgtg ccgccgggct acgtccgctt
     5341 ttaatcacca gaatcggttt attacgcgag gcactacggg ccgccgaaac aaagcgtcgc
     5401 gcgtcgctta actgttcgag atagagcagg atggcgctgg ttttactgtc gcgcgccaga
     5461 tagtcgagca attcatcaac gtcgatatct aggctgtcgc cgagcgcaat aaagtaggaa
     5521 aagcccatct cacgctgttg cgcccagtcg aggatggtgt tggagacggc agccgattgc
     5581 gaaataaacg ccagcttgcc gcgtttaatc ggcacaggcg aaaagctggc attcagacct
     5641 tgccagggag ccagtaaacc cagactgttt ggtccaagca ggcgcatgtt atggcgcagg
     5701 gcgcaggcgc ggagatcttc gtgttgcgat gccggggcgg aaagaataat gcaggtttta
     5761 cagcctttct cgccgagctc ttccagaaga gcaagattac ggctggcatt ggtacataaa
     5821 accgcaaggt cgggtgtaaa gggcaagctg gcaatatccg gccaggccaa cacacccaac
     5881 accgctttcc aggctggcgt caccgggagt accggtccgt taaagcctcc cgccagcagg
     5941 ttacgcatca tcaggtaacc tgcgcgattg ggtttcatcg acgcgccaat taccgctatc
     6001 gattttggtc gcagtagtgc ttccagtcct cgctgactca taccggtctc ccgcagaagt
     6061 gaccgaatga ttttaaacgc tttctaactg ttctgctgtg atgctaccca gatgttgcgt
     6121 ttttcctgcc agatagcgtg ttttaaagcg ggtaaaatgc tcgcctaacc ctgctgccgc
     6181 cccggtatcg ccggccatat ctaacagtgc gatggctacc tcggcagtac aatattggcc
     6241 tgcagcctgg gcttcacgca ggcgataggc agaaagccgg gaaagatcga cggaaatgac
     6301 gggaagatta tccagatacg gacttttacg aaacatcttg cgagcttccg gccaggtacc
     6361 atcgagcatg ataaacagcg gtggcttacc ggcaggtggt gtgaagatca cttcccgttg
     6421 ctcatcagca tacgaggcgg gaaagaccac cattggctga taatccgggt tttgtaccag
     6481 atccagcaaa tcctgcgagg gttcggtacg cgaccattga aacgcaacgg tatcaggcaa
     6541 aatatcagca atcagacgcc cggtattact gggcttcatt ggctcggtgt cgaacatcag
     6601 caaacagaag cgactttttg cttgtgctgg ggtaattgtc gaacagagac ataatttctc
     6661 tggcaaaaga cagcgttggc agcgacgaac gcgattaccg cgggcaagaa aaggacgtgt
     6721 tgcgcgcgca atacgctcgg cgcgtaactg gagaacagcg ttttcggtca taagagagcg
     6781 tcgaaaaaac gccattgtcg cagaggagaa aacggggcac aagatgcgcc ccggtaagat
     6841 taaagagatt cgttcagcca gctatcgaac ggcgctttcg gtactgcgcc attaagcatg
     6901 tcgacaacct gaccgttttt gaaaatcatg atcgtcggta tactacgaat tccaaagcga
     6961 ctgctcaatt cacgttcagc ttcggtattc actttcacaa agcgcacttt accgctacgc
     7021 tcttgcgcga catcttcaaa aattggtgcg aaattacggc aggggccgca ccacggtgcc
     7081 cagaagtcga tcaccacagg tagatcatcc ttcagcaatt tgtcgagtgt ttcaccggtc
     7141 gcattaatca cctctccgtc aaacaagtcg tgaccgcagc gtccgcattt tgccgcatct
     7201 tcgatccgat cgtcgggaat gcgattgatg gcctgacaat gggtacaaac ggtattcata
     7261 actaacctcg ggatgagtaa ggagacttat cggcaacgac gccggtatgt ttctaatatg
     7321 ttacatatta tcggggacat tgttccagac aacaatctgt tttattcaat aggtatgata
     7381 gtcgcaggct ttgctacgaa atcatggcga accgcgcgga cttcgggtaa tctgcgcgct
     7441 tcgcgcagcg ctggtggaga aaagcatgaa cgatgaaatg aaaggtaaaa gcggcaaggt
     7501 caaagtgatg tatgtccgca gtgatgatga ttctgataaa cgtacccaca acccgcgtac
     7561 cgggaaaggg ggcgggcgtc caggaaaatc tcgtgctgat ggtggccgtc gccccgcccg
     7621 cgatgacaaa cagagtcagc cccgtgaccg caagtgggaa gattcgccgt ggcgcacggt
     7681 ttcccgcgcg ccgggtgatg agacgccgga aaaggccgat cacggtggca tcagtggtaa
     7741 aagttttatt gatccggaag tgttgcgtcg tcagcgtgcg gaagaaaccc gcgtctacgg
     7801 cgaaaacgcc tgtcaggcac tgttccagag ccgtccggaa gcgattgttc gcgcctggtt
     7861 tatccagagt gtaacgccgc gttttaaaga agccttgcgc tggatggcag caaaccgcaa
     7921 agcgtaccat gtggtggatg aagcggaatt gacaaaagcg tcaggtacgg aacatcacgg
     7981 cggcgtttgc ttcctgatca aaaagcgtaa cggtacaacc gtgcagcagt gggtaatcca
     8041 ggcaggcgcg caggattgcg ttctggcact ggaaaacgaa tctaacccgc ataacctggg
     8101 tggcatgatg cgcagctgcg cgcacttcgg cgtgaaaggt gtcgtggtgc aggatgcggc
     8161 actgctggag tcgggggcgg ctatccgtac cgcagaaggc ggcgcagagc acgttcagcc
     8221 gattactggc gacaacattg ttaacgtgct ggatgatttc cgtcaggcag gttacaccgt
     8281 agtgaccact tccagcgagc agggtaaacc gctgttcaaa accagtctgc cagcgaaaat
     8341 ggtactggtg ctgggtcagg aatatgaagg gttaccggat gccgcacgcg atccgaacga
     8401 tctgcgcgtg aagattgacg gtacgggcaa cgttgccggg ctgaatatct ctgtcgcaac
     8461 cggcgttttg cttggtgaat ggtggcgtca gaataaagcc tgataaatca gccgggtggc
     8521 aactctgcca tccggcattt ccccgcaaat ttactcacac tctgccggta atactggcat
     8581 ccagtcaatc ggcgtctcgc cacgttgttc cagccactga tttgccagca caaaatggtt
     8641 gcagccaaag aatccacgat gcgccgaaag cggcgacgga tgcggtgctt tcagtacatg
     8701 atggcgttgc ttatctataa tcgccccttt cttttgcgca tgtgatcccc acaacaaaaa
     8761 caccacgcct tcgcggtgct ggttaatcag gctgatgact ttatcagtaa acgtctccca
     8821 accgaggctg gcgtgggaat gcgcctgacc tgcgcgaacc gtcaacacag tattgagcag
     8881 cagaacgccc tgacgcgccc agctttcgag atagccatga ttagggcggg tgaagcccgg
     8941 aatagtattt tccagctctt tatacatatt caataatgac ggaggaatgg caatgccggg
     9001 acgaacggaa aatgccagac catgcgcctg tcccggtccg tgataaggat cctggccgag
     9061 aatcaccact ttaacgtcac ccaactcggt aaagcggaac gcgttaaaga catctttttg
     9121 tggtggatag atagtgacgc cggactgccg ctcgctggcg acggtctgaa gggtattaag
     9181 aaaataaggt tgttgcttct cttcagccag cacgtcatgc caggttaatt cgttagccat
     9241 cttgctctcc tgcgaatctt caatccgcct agcttaactg cttctttcta cagaacaaaa
     9301 tcatgctcat tttttagact taaacgagtg caaaaaaatg taaattggta aaatttcaca
     9361 cccgcataag ttgatgtaaa acaataaaat ccatttacga ccacaatcga tgtgtggttt
     9421 ttgttgattt aaatcaaaga atcaagggtg gttgaggagt atatatacac tcaagcaaca
     9481 atggttttac caattggccg cgacaggctg aacaaatcaa ataattttgc cggggaggca
     9541 tcacatgatt acaggtatcc agattactaa agccgctaac gccgatctgc tgaactcttt
     9601 ctggctgctg gacagcgaaa aaggcgaagc acgttgcatc gttgcaaaag caggttatgc
     9661 agaagatgaa gtggttgcag taagcaaact gggtgacatt gaataccgtg aagttccagt
     9721 agaagtgaaa ccagaagttc gcgttgaagg tggtcaacac ctgaacgtta acgttctgcg
     9781 tcgtgaaact ctggaagatg cagttaagca tccggaaaaa tatccgcagc tgaccatccg
     9841 tgtatccggt tatgcagttc gctttaactc tctgactccg gaacagcagc gcgacgttat
     9901 cgctcgtacc tttactgaaa gcctgtaagg cttaagtagc aagttactaa gcggaagagg
     9961 gcgacctctt ccgctttttt tcgttaatag aaaatgcgta ccgcgcaata gaccagcaac
    10021 agggcaagca cgatatttaa ctggcgacca tactggcgaa acaatcgctg aaacagatgc
    10081 cccgccagcg cccagcacac attgccaaac gtcccaatca tcgccagcaa aacgctgacg
    10141 ccaactaccc agcttagcgc ctgtgtttgc ggcagaacaa acgtcgacag tgccgtaaca
    10201 ccgtacaaaa tgattttgac gttcacaaac tgcaaagcaa agctggccca aaagctgatt
    10261 ggttttgcct gaagtccgtc ttcctttgtt gggctggtgg cgattttcca cgccagccag
    10321 acaatatatg ccgcccccgc ccaactcaaa aggtgtaccg ctgccgggtc aatcactgcc
    10381 agtgaaaatg aaatgcccgc acacagtaac atcacaatca aaaatcccag actcatccct
    10441 gccagcacgc gggtactttg acgaaatcca tgcgacgtag cagagctaag ggcgagaata
    10501 ttgttcggtc ctggcgtcat agcggtaatc agggtgtaag tccaaaaagc acttaaaagg
    10561 atcggtgtca cttgttatct cctctgtttt ggtagacagt acccgacacg gaacatttaa
    10621 aaaaatgaat gtttttatat tctaaattcg atattttcga taggtttggg gttatggatc
    10681 tgcgccgttt tattacgctt aaaaccgtgg tggaagaggg ttcctttttg cgagcttcgc
    10741 aaaaattgtg ctgtacacaa tcgacggtga cttttcatat tcagcagctt gagcaggagt
    10801 tttcagtcca gttatttgag aaaattggcc gacggatgtg ccttacccgc gaaggaaaaa
    10861 agttgctgcc gcacatttat gaacttaccc gagtgatgga tacgcttcgt gaggctgcca
    10921 aaaaggagtc agatccggac ggggaactac gcgttgtttc gggcgaaacg ttgctctctt
    10981 accgaatgcc gcaggtgttg cagcgttttc ggcaacgcgc accaaaagtg cgtttatcgt
    11041 tgcaggcgct gaactgttac gtgatccgtg atgcgctact gaatgatgag gccgatgtcg
    11101 gcgtctttta tcgcgtaggg aatgatgatg ccctgaaccg acgagagttg ggtgaacaat
    11161 cgctggtgct ggtggcttca ccacaaattg cagatgtcga ttttactgag ccgggaagac
    11221 ataacgcctg tagctttatt atcaacgaac cgcaatgtgt cttccggcag atatttgaga
    11281 gcacgctgcg acagcggcgg atcacggtgg aaaacaccat tgagcttata agtatcgaaa
    11341 gcatcaagcg ttgtgtcgcg gcgaatatcg gtgtcagtta cttgccccac tttgcggtgg
    11401 aaaaagagat tgagagcgga gaattaattg aattgccttt tggcgaacag tcgcagacca
    11461 ttacggcaat gtgcgcacat tatgctggaa aagcggttag cccggcgatg cacattttta
    11521 ttcagtgtgt tgaagagtgt tttgtggttg cgtaaaaaaa caatgccggg cttaaacccg
    11581 gcattgagat tactcttctg tcgtttgcgg tggcacgccc gttccgctcg gtttacggcg
    11641 cttaccaata tttttggtgt cgcgatggcg ttttttcacc cgcggcttct ctttctcttt
    11701 agcttttttc ttctcggcgc gttttgccag tactttcttc gatggcttgc cggtctgctt
    11761 ctcgcttggc gcacgcgttt tcgggcgtaa ctcatcaata acgcgagctt taatcggctc
    11821 ttcaatgtaa cggccaacct tgcccagcag caaatggtca tgggcttcca ccagcgaaat
    11881 tgcggtgcct ttacgaccgg cgcgcgcggt acgtccgata cgatgcaaat aagtatcgcc
    11941 actgcgcggc atatcgaagt taaagacgtg gctgacgtca ggaatgtcga taccgcgcgc
    12001 ggcaacatcg gtggcaacca gtacgtttac gcggccttcg gtcaggcgtt taattgcttc
    12061 gttacgcttg ccttgtacca tctcaccttc gagatagcag ttgttgatgc ccgcttcgcg
    12121 cagccagttt gccagctcat gcacacgttc acgcttacgc acaaacacga ttgagcgagt
    12181 cgcttcaggc tgttttaaca ggtgcaccag caatgcggtt ttatgttcca ggtcatcggc
    12241 gcggtaatac cactgatgaa tttttttgcg ctcacgggtg gacggattgg cagaaacttc
    12301 caccggatct tccagcagac gctcggcaaa gtcctgaatc gcatcgcctt ccagcgttgc
    12361 cgaaaagagc agggtctgtt tacgccagcg agtttcgcca gcaatatgtt cgatatcctg
    12421 agcgaagccc atatccagca tacggtctgc ttcgtcgagg atcagcgttt caaccgcgcg
    12481 gcaatcgaag ttctcttctt ttatgtattg cagcagacgt ccggtcgtgg cgaccacgat
    12541 gtcctgattt tcgctgaaca cttccgcgtg gttcatatag gctacgccgc cggtgatggt
    12601 ggcgatatcc agatgcgtat gtttcgccag ttcgcgggca tgatcggcca cctgcatcgc
    12661 cagctcgcga gttggggtga ggatcaaaat acgcggcgga ccggatttct tacgtgggaa
    12721 atcgagcagg tgctgcaacg ctggcagcag atacgccgcc gttttaccgg tgcctgtcgg
    12781 cgcagaaccg agtacatcac ggccatcgag cgcaggcgga atggcggcag cctgaatggc
    12841 ggtcgggcga gtgaaacctt tatcctggag ggcttccagc aggctttcgt cgagttcaag
    12901 ttcggaaaaa gtcgttacag tcatgttcta cctctgtgtg gggcgctgat tatagacgtt
    12961 acggctgtaa tcttcatctg tttgtatgga tatcgcttct cgggtattgt tctcacccgg
    13021 tgctatcgcc gtttttcctg caaggtttta ttttcatgtc acagtctaca tccgtgcttc
    13081 gtcgtaatgg atttactttt aaacagtttt ttgttgctca cgatcgctgt gcgatgaaag
    13141 tgggaacgga tggcattttg ctgggcgcat gggcaccggt ggctggggtg aaacgttgcc
    13201 ttgatatcgg cgcgggtagc gggttgctgg cactgatgct ggcgcagcga accagtgaca
    13261 gcgtgataat tgatgccgtt gagctggaaa gtgaagctgc cgcgcaggcg caggaaaata
    13321 tcaaccagtc cccgtgggca gagcggatta acgtccatac ggcggatatt cagcagtgga
    13381 tcacacagca gacagtacgc ttcgatttaa tcatcagtaa cccaccttac tatcagcagg
    13441 gagtagagtg cgcgacacct caacgggaac aggctcgcta taccactacg cttgatcacc
    13501 catcgctgct gacctgtgcg gcggagtgca ttaccgaaga gggatttttc tgcgtggtgc
    13561 tgccagagca gattggtaat ggttttactg aactggcatt aagcatgggc tggcatttac
    13621 gtttgcggac ggatgtggcg gaaaacgaag cgcgactgcc gcatcgggtg ctgctggcat
    13681 tctccccgca ggcgggagaa tgctttagcg atcgcttagt cattcgtggg ccagaccaga
    13741 actattccga agcgtatacg gcgctgaccc aggcttttta tctgtttatg taatgattgc
    13801 cgggggaaag gatcgatgga ccggaatgcg tgagcagttc cgggtaatcc agcgtaaaat
    13861 gcagcccccg gctttctttg cgcatcatcg cgcagcgaac aatcaactcg gcaacctgta
    13921 ccagattacg cagctccagc aaattatttg agacgcggaa atgggcgtaa tattcgtcta
    13981 tttcttgttg gagcatggtt atccgccgca gggcgcgttc caggcgcttc gttgtgcgca
    14041 caatgccaac gtaatcccac ataaacagcc gtagctcgtg ccagttatgc tgaattacta
    14101 cccgttcgtc agggttctca acgcggcttt catcccacgg cggtaacgta ctgacgccgt
    14161 gggcataagg catacgtctg gtgatatctt ctgccgccga ccagccatag accagacact
    14221 ccagcaatga attcgaggcc atgcggttag cgccgtgtaa gccggtataa ctcacctcgc
    14281 caatggcata caagccctcg acgtccgtac gcccatgatc atcaaccatt acaccacctc
    14341 aggtatagtg tgcagcaggc acaatcggta ccggttcttg tgtgagatca atccccagcc
    14401 cgagcaattt ttcataaatc atcgggaaat gctggcgaat aaaatcggcg ggcttatggc
    14461 tgatgtcgag gaacatacaa tctgcgccga ggcgtttcat ttcatggtca atggcgcggg
    14521 cgacaatatc gcgcggggcc agttcgccgc gctcatcaaa atcgggcata aaacgcgtgc
    14581 catccgggcg cttgagataa gcgccttcgc cgcgcagtgc ttccgttaac aggaaattgc
    14641 gtgcctgtgg gtgatacagt gcggtagggt ggaactgatt aaattcgaga ttggcaaccc
    14701 gacagcccgc gcgccacgcc atagcaatgc catcgccaga agaaatatcc ggattggtgg
    14761 tgtactgata caccttcgac gcaccgccgg ttgccagtac cactgctttt gcgtggcagg
    14821 tttccaccgt ttctttattt cggttccata cccacgcgcc tacaacccgt cgcgtgcccg
    14881 gcaggccaat tttgtcagag acaatcagat caactgcgtt gctgcgctcc agcacacgaa
    14941 tattcggatg gttctgcgcc ttgctcacca gcgtggattg tacttctcta ccggtggcgt
    15001 cggcggcatg aagaatacga cggtgactat gtccaccttc acgggtcaga tggtagcttt
    15061 cttcgccatt cggttgaacg tgggtatcaa acaaaacccc ctggtcaatt aaccatggca
    15121 cacaggaacg agcattgctg gcgacaaatt caactgcatg gtgatcgcaa ataccagccc
    15181 cggcaatcaa tgtgtcttcc acatgcgagt caatgctgtc agtttcatca aacacggcgg
    15241 caataccgcc ctgggcataa aatgttgaac cttccgttac cgggccttta cttagaacga
    15301 tgacctgatg ctggtcagcc aggcgtagcg ccagtgaaag tccggctgcg ccgctaccaa
    15361 taatcaacac gtcacatgaa tgttcaggga gagtattcat tttctttgtt taatttacta
    15421 aacattgttt ggtcggcata gcatcatgtt gtgcgaataa acacctgcta ttttaatatt
    15481 tgttacagtg gctaaacacg ttgacgcagg gcggcgagaa aaagagaagt tactggctgg
    15541 tggaggatta ggtggtgaaa taaaaaggcc gttgggttac tcttcaggca gttaaatggg
    15601 catttctaca cagataatgc gatgttcaga ttctgtagac ttataatgat agataatgat
    15661 ccgtctacag catgacaaac aaaaacagat gcgttacgga actttacaaa aacgagacac
    15721 tctaaccctt tgcttgctca aattgcagct aatggagtgg cgtttcgata gcgcgtggaa
    15781 atttggtttg gggagacttt acctcggatg agcgagcagt taacggacca ggtcctggtt
    15841 gaacgggtcc agaagggaga tcagaaagcc tttaacttac tggtagtgcg ctatcagcat
    15901 aaagtggcga gtctggtttc ccgctatgtg ccgtcgggtg atgttcccga tgtggtacaa
    15961 gaagctttta ttaaagccta tcgtgcgctg gattcgttcc ggggagatag cgctttttat
    16021 acatggctgt atcggattgc tgtaaataca gcgaaaaatt acctggttgc tcaggggcgt
    16081 cgtccacctt ccagtgatgt ggatgccatt gaagctgaaa acttcgaaag tggcggcgcg
    16141 ttgaaagaaa tttcgaaccc tgagaactta atgttgtcag aagaactgag acagatagtt
    16201 ttccgaacta ttgagtccct cccggaagat ttgcgcatgg caataacctt gcgggagctg
    16261 gatggcctga gctatgaaga gatagccgct atcatggatt gtccggtagg tacggtgcgt
    16321 tcacgtatct tccgagcgag ggaagctatt gataacaaag ttcaaccgct tatcaggcgt
    16381 tgacgatagc gggatactgg ataagggtat taggcatgca gaaagaacaa ctttccgctt
    16441 taatggatgg cgaaacgctg gatagtgagc tgcttaacga actggctcat aacccagaaa
    16501 tgcagaaaac ctgggaaagc tatcacttaa tccgtgattc aatgcggggt gatactcccg
    16561 aggtgctcca tttcgatatc tcttcacgcg tgatggccgc cattgaagaa gagccagtac
    16621 gtcaatcggc gacattgatc ccggaagccc agcctgcgcc gcatcaatgg cagaaaatgc
    16681 cattctggca gaaagtacgt ccgtgggcgg cacagcttac ccaaatgggc gtagccgcat
    16741 gcgtatcgct tgcagttatc gttggcgtcc agcactataa tggacaatct gaaacgtccc
    16801 agcagcccga aacgccggta tttaatacac tgccgatgat gggtaaagcc agcccggtaa
    16861 gcctgggagt accttctgaa gcgaccgcaa acaatggtca acagcagcag gtacaggagc
    16921 agcgtcgtcg cattaatgca atgttgcagg attacgaact gcaacgccga ctccactctg
    16981 aacagcttca gtttgagcag gcgcaaaccc agcaagccgc tgtacaggtg ccaggaattc
    17041 aaactttagg aacgcaatcg cagtaatgaa gcaactttgg tttgccatgt cattagtgac
    17101 aggtagcctg ttattctctg ctaacgcctc ggccactccc gcgtccgggg cgttattaca
    17161 gcagatgaac ctggccagtc agtcactgaa ttacgagctg tcattcatca gcatcaataa
    17221 acagggtgtt gagtctctgc gttatcgaca tgcacgcctc gataaccgtc ctcttgcaca
    17281 attgttgcaa atggatggcc cgcgccggga agtggtacag cgcggcaatg aaatcagcta
    17341 ttttgaaccg ggacttgaac cgttcacgct taatggcgat tacattgttg attctctgcc
    17401 atcgcttatc tataccgatt tcaaacgcct ttctccttac tacgacttta tctccgtcgg
    17461 gcgcacgcgt attgctgatc gtctttgcga agtcattcgc gtggttgccc gagatggtac
    17521 acgctacagc tacatcgtgt ggatggacac cgaatcgaaa ttaccgatgc gggttgatct
    17581 ccttgatcgc gatggtgaaa cgctggaaca atttcgcgtg attgctttta acgtcaatca
    17641 ggatatcagc agcagtatgc agacgctggc gaaggcaaat ttgccgccgt tgctttctgt
    17701 tcctgtaggt gaaaaagcta aattcagctg gacgccaacc tggttgccac agggttttag
    17761 cgaagtttcc agtagtcgac gtccgctacc gacgatggac aacatgccta tcgaatcacg
    17821 tctctattcc gacggattat tcagcttctc ggtaaacgtt aaccgcgcta cgccatcgag
    17881 caccgatcag atgttgcgca ccggacgcag aaccgtcagt acaagcgtac gtgataacgc
    17941 cgaaatcacc attgtcggtg aactgccgcc gcaaacggcg aaacgcattg ccgagaatat
    18001 taagttcggg gcagcgcaat gatcaaagag tgggctaccg tcgtctcctg gcaaaacggg
    18061 caggcgctgg tcagttgtga tgttaaagct tcatgcagca gctgcgcttc acgcgccggt
    18121 tgcggtagcc gcgtgttgaa taaacttggc ccgcaaacca cgcataccat tgtcgtaccc
    18181 tgtgatgagc cgttagtgcc ggggcaaaaa gtggagttgg ggatcgccga aggcagcctg
    18241 cttagctccg cattactggt ttatatgtcg ccgctggtgg gattattcct catcgcttcg
    18301 ctatttcagc tactctttgc ttctgacgtc gcagcattat gtggtgcggt cctcggcggt
    18361 attggcgggt tcctgattgc gcgcggctac tcgcgtaagt ttgctgcccg ggcagaatgg
    18421 caaccgatca tcttaagcgt ggcattgccg cctggccttg tgcgatttga aacatcttcg
    18481 gaagacgcga gccagtgatt tcactggctt tgttgcattt tgcataacaa atgcgaggac
    18541 gtttccagat accacaatcc atacggtatg aacatttcct cgcctcaatg ttgtagtgta
    18601 gaatgcggcg tttctattaa tacagacgtt aagctcagaa cagcgacttc taaaagcctg
    18661 gttaaccggg cattaaggca caataatcat actttatgaa gaatatacgt aacttttcga
    18721 tcatagctca cattgaccac ggtaaatcga cgctgtctga ccgtattatc cagatctgcg
    18781 gtggcctgtc tgaccgtgaa atggaggcgc aggttctcga ttccatggat cttgagcgtg
    18841 agcgtggcat taccatcaaa gcgcaaagcg tgacgctgga ctacaaagcg tctgacggcg
    18901 aaacctatca gcttaacttt atcgacaccc cgggccacgt agacttctcc tatgaagttt
    18961 cccgttcgct ggctgcctgt gaaggtgcat tgctggtggt cgacgccggg cagggcgtag
    19021 aagcgcaaac cctggcaaac tgctacaccg ccatggaaat ggatctcgaa gtggtgccgg
    19081 tactgaacaa gattgacctg ccggcagccg atcctgaacg cgtggcggaa gaaattgaag
    19141 atatcgtcgg catcgacgcc accgatgcag tgcgctgttc agcgaaaacc ggcgttggcg
    19201 tgcaggacgt tctcgaacgt ctggtgcgcg acattccgcc gccggaaggc gatccggaag
    19261 gcccgttgca ggcactaatt atcgactcct ggttcgacaa ctacctgggc gttgtttcac
    19321 ttatccgtat taaaaacggc accctgcgta agggcgacaa agtgaaagtc atgagtaccg
    19381 ggcagaccta taacgccgac cgtctgggca tcttcacgcc gaaacaggtt gaccgcactg
    19441 aactgaaatg tggcgaagta ggctggctcg tatgtgcgat taaagatatc cacggcgctc
    19501 cagtcggcga taccttaacg ctggcgcgta atccggcaga aaaggcgctg cctggcttta
    19561 agaaagtcaa accgcaggta tacgccggtc tgttcccggt aagttccgac gactatgaag
    19621 ccttccgtga cgcgctgggt aaactcagcc tgaacgatgc ctcactgttc tatgagccgg
    19681 aaagctccag cgcgctgggc tttggtttcc gctgcggctt ccttggcctg ctgcacatgg
    19741 agatcatcca ggagcgtctg gaacgtgaat acgatctgga tctgatcacc actgcgccga
    19801 ccgtagtgta tgaagttgaa accacgtcaa gggaagttat ctacgtcgac agcccatcca
    19861 agctgcctgc ggtaaataac atttacgaac tgcgcgagcc gattgcagag tgtcacatgc
    19921 tgctgccgca ggcatatctc ggcaacgtta ttacgctgtg cgtagaaaaa cgcggcgtgc
    19981 agaccaatat ggtttaccac ggtaatcagg tggcgctgac gtacgagatc ccgatggcgg
    20041 aagtggtgct cgacttcttc gatcgcctga aatctacctc gcgtggttat gcgtctctgg
    20101 attacaactt caaacgcttc caggcgtccg acatggtacg tgtagacgta ttaatcaacg
    20161 gtgaacgtgt tgatgcgctg gcgttgatca cccaccgtga taattcgcaa aaccgcggtc
    20221 gcgagttggt ggagaagatg aaagatctga tcccacgcca gcagtttgat atcgccattc
    20281 aggcagcgat tggtacgcac attattgcgc gatccaccgt gaaacagctg cgtaaaaacg
    20341 tactggctaa atgttatggc ggcgatatca gccgtaagaa aaagctgctg cagaagcaga
    20401 aagaaggtaa gaaacgcatg aagcagatcg gtaacgttga gctgccgcag gaagcgttcc
    20461 tcgccattct gcacgtcggc aaagacaaca aataaccctt aggagttggc atggcgaata
    20521 tgtttgccct gattctggtg attgccacac tggtgacggg cattttatgg tgcgtggata
    20581 aattcttttt cgcacctaaa cggcgggaac gtcaggcagc ggcgcaggcg gctgccggtg
    20641 actcactgga taaagcaacg ttgaaaaagg ttgcaccgaa gcctggctgg ctggaaaccg
    20701 gagcttctgt ttttccggtg ctggctatcg tattgattgt acgttcgttt atttatgaac
    20761 cgttccagat cccgtcaggt tcgatgatgc cgactctgtt aatcggtgat tttattctgg
    20821 tagagaagtt tgcttatggc attaaagatc ctatctacca gaaaacgctg atcgaaaccg
    20881 gtcatccgaa acgcggcgat atcgtggtct ttaaatatcc ggaagatcca aagcttgatt
    20941 acatcaagcg cgcggtgggt ttaccgggcg ataaagtcac ttacgatccg gtctcaaaag
    21001 agctgacgat tcaaccggga tgcagttccg gccaggcgtg tgaaaacgcg ctgccggtca
    21061 cctactcaaa cgtggaaccg agcgatttcg ttcagacctt ctcacgccgt aatggtgggg
    21121 aagcgaccag cggattcttt gaagtgccga aaaacgaaac caaagaaaat ggaattcgtc
    21181 tttccgagcg taaagagaca ctgggtgatg tgacgcaccg aattctgaca gtgccgattg
    21241 cgcaggacca ggtggggatg tattaccagc agccagggca acaactggca acctggattg
    21301 ttccgccggg acaatacttc atgatgggcg acaaccgcga caacagcgcg gacagccgtt
    21361 actggggctt tgtgcctgaa gcgaatctgg tcggtcgggc cacggctatc tggatgagct
    21421 tcgataagca agaaggcgaa tggccgactg gtgtgcgctt aagtcgcatt ggcggcatcc
    21481 attaatagcc atcttcgttc acgttgtcgc cgttatggcg acaacgtgaa ttatttatga
    21541 gataaatctc ccgtggctaa cgacatcccc cgtcgttgtg tatagaatat tcccccgaag
    21601 tttaaggttg gcaccttcag gttgccacgg cacacgaaac agcgttggtc cccatatacc
    21661 gctaaactga aactgcagcg aagcagttag cagagccatg tatatcaggt ctgtttcgtg
    21721 tgctgaattg ttgacgcatt tatttattgg tatcgcatga accccatcgt aattaatcgg
    21781 cttcaacgga agctgggcta cacttttaat catcaggaac tgttgcagca ggcattaact
    21841 catcgtagtg ccagcagcaa acataacgag cgtttagaat ttttaggcga ctctattctg
    21901 agctacgtta tcgccaatgc gctttatcac cgtttccctc gtgtggatga aggcgatatg
    21961 agccggatgc gcgccacgct ggtccgtggc aatacgctgg cggaactggc gcgcgaattt
    22021 gagttaggcg agtgcttacg tttagggcca ggtgaactta aaagcggtgg atttcgtcgt
    22081 gagtcaattc tcgccgacac cgtcgaagca ttaattggtg gcgtattcct cgacagtgat
    22141 attcaaaccg tcgagaaatt aatccttaac tggtatcaaa ctcgtctgga cgaaattagc
    22201 ccaggcgata aacaaaaaga tccgaaaacg cgcttgcaag aatatttgca gggtcgccat
    22261 ctgccgctgc cgacttatct ggtagtccag gtacgtggcg aagcgcacga tcaggaattt
    22321 actatccact gccaggtcag cggcctgagt gaaccggtgg ttggcacagg ttcaagccgt
    22381 cgtaaggctg agcaggctgc cgccgaacag gcgttgaaaa aactggagct ggaatgagca
    22441 tcgataaaag ttactgcgga tttattgcca tcgtcggacg tccgaacgtt ggcaaatcca
    22501 cattgttgaa caaactgctg gggcagaaaa tctccatcac ctcccgcaag gcgcagacaa
    22561 ctcgtcaccg cattgtgggg atccatactg aaggcgcgta tcaggcgatc tacgtcgata
    22621 caccgggcct gcatatggaa gaaaaacgcg ccattaaccg cctgatgaac aaagcggcga
    22681 gcagctctat tggcgatgtt gagctggtga tttttgtcgt tgaaggcacc cgctggacgc
    22741 cggacgacga aatggtgctc aacaaactgc gcgaaggcaa agcgccggta atcctcgcgg
    22801 tgaacaaagt ggacaacgtg caggagaaag ccgatctgct gccgcacctg cagttcctgg
    22861 caagccagat gaacttcctc gatatcgtgc caatctctgc cgaaaccggg ctgaatgttg
    22921 acactattgc ggcaatcgtg cgtaagcatc tgcctgaagc gactcatcac ttcccggaag
    22981 attacatcac cgatcgctca cagcgtttta tggcgtctga aatcatccgc gaaaaactga
    23041 tgcgtttcct tggcgctgaa ctgccgtact ccgtgaccgt ggagatcgaa cgtttcgtct
    23101 ctaacgaacg cggcggttgt gacatcaacg gtttgattct cgttgagcgt gaagggcaga
    23161 agaagatggt tattggcaac aaagggtcca agatcaaaac catcgggatt gaagcgcgta
    23221 aagacatgca ggaaatgttc gaagcgcctg tccaccttga actgtgggta aaagtgaaat
    23281 cgggttgggc cgacgacgaa cgcgcactgc gcagtctcgg ttatgttgac gatctttaag
    23341 agtcactccg atggaaggct ggcagcgcgc atttgtcctg catagtcgcc cgtggagcga
    23401 aaccagcctg atgctggacg tcttcacaga ggaatcgggt cgcgtgcgtc tggttgccaa
    23461 aggcgcacgc tctaaacgct ctactctgaa aggtgcatta cagcctttca cccctctctt
    23521 gctacgtttt ggcgggcgtg gcgaagtcaa aacgctgcgc agtgctgaag ctgtctcact
    23581 ggcgctgcca ttaagcggta tcacgcttta cagcggtctg tacatcaacg aacttctctc
    23641 ccgcgtactg gaatacgaga cgcgcttctc tgaactcttt ttcgattact tgcactgcat
    23701 tcagtctctt gcaggtgaca ctggtacgcc agaacccgcg ctgcgtcgct ttgaactggc
    23761 actactcggg catctgggct atggcgtcaa ttttacccat tgcgcgggta gcggcgagcc
    23821 ggtagatggc accatgacgt atcgttatcg cgaagaaaaa gggtttatcg caagcgtcgt
    23881 tatcgacaat aaaacgttca ccggaaggca gttaaaagcg ttaaacgcgc gggaatttcc
    23941 tgacgcagac acactacgcg ccgcgaaacg ctttacccga atggcgctta agccgtatct
    24001 tggcggtaaa ccattaaaga gcagggaact gttccggcag tttatgccta agcgaacggt
    24061 gaaaacacat tatgaatgat gaggattgtc atggctgaat tactgttagg cgtcaacatt
    24121 gaccatattg caacgctgcg taacgcacgc ggtactgctt acccggatcc ggtgcaggcg
    24181 gcgtttattg ccgagcaggc gggagcggac ggcattaccg tgcatttacg tgaagatcgc
    24241 cgccacatta ctgaccgcga tgtgcgcatc ctgcggcaga cgctggatac ccgaatgaat
    24301 ctggagatgg cagtgaccga agagatgctg gcgatcgccg ttgagacgaa gccacatttt
    24361 tgctgcttgg taccggaaaa gcgtcaggaa gtaacaaccg aaggcggcct ggacgttgca
    24421 gggcagcgtg aaaaaatacg cgatgcctgc aaacgtctgg cagatgccgg gattcaggtt
    24481 tctctgttta tcgacgccga tgaagagcag atcaaagctg cggcagaggt tggcgcaccg
    24541 tttatcgaga tccacaccgg ttgctatgct gatgccaaaa ctgacgccga acaggcgcaa
    24601 gagctggtgc gtatcgccaa agccgcgacc tttgccgcta gtctcggtct gaaagttaac
    24661 gccggacacg gtctgaccta tcacaacgtg aaagccattg ccgccatccc tgagatgcat
    24721 gaactgaata tcggtcatgc cattattggc cgtgcagtga tgaccggact gaaagatgcg
    24781 gtggcagaaa tgaagcgtct gatgctggaa gcgcgtggct aatggcaata ttaggtttag
    24841 gcacggatat cgtagagatc gctcgcatag aagcagtgat cgcccgatcc ggtgagcgcc
    24901 tggcacgccg cgtattaagc gataacgaat gggcaatctg gaaaacgcac caccagccgg
    24961 tgcgttttct ggcgaagcgt tttgctgtga aagaagccgc agcaaaagcg tttggcaccg
    25021 ggatccgcaa tggtctggcg tttaatcaat ttgaagtatt caatgatgag ctcggcaaac
    25081 cacggctacg gctatggggt gaggcattaa aactggcgga aaagctcggc gttgtaaata
    25141 tgcatgtaac gctggcagat gaacgccact atgcttgtgc cacggtaatt attgaaagtt
    25201 aaaataatac cgttctcttt aaaaagtaaa acccccgaac tggtaaggaa cgggggtttt
    25261 actttttaac tttaattttg tgcagcatgc accctcaaca acagcgatta tggatcagtg
    25321 tacttatctt ttacaagcaa cttgcaatct ttagcataaa aactcgagcc tttacgaaga
    25381 aagcaatatt gatggaaaga ttaacgtgac cgccaattcg taagtacatt aaaattggct
    25441 tcgttattga agattttgct gtgctttaca ccatgccaca gaattccccc attgaaacga
    25501 gtggtgtcgt caaagctctg gtgtggagtg cagcatgcac cctcaataac tcgcacgttc
    25561 agttttgggg agatgtaagg gctaatctga atggctgcat tccttgttta aggaaaaacg
    25621 aatgactgat tgccgatacc tgattaaacg ggtcatcaaa atcatcattg ctgttttaca
    25681 gctgatcctt ctgttcttat aacacaagga aacgtactta aggtgcgtcc ggtgaaccaa
    25741 tcggacgcgc ctttaataac tataaataag tgtcttggca gatactatat aaattaactt
    25801 agtgaatgat tatgctaatg tcatcaatta aataaatata atggcgttaa ggcttcccag
    25861 taatataatt aatactctac ttccagagta gaatattaaa ttttatccgc gtggtgcatc
    25921 agcacaaatt tatcccacaa ctgttcttct gtctcgacat gcgccggatc tttcacaata
    25981 gtattgggga tcgggcacac cttctggcag gttggtgtct cgtagtgccc tacgcattcg
    26041 gtacacttat cgctgttaat ttcgtagata tgatctccca ttgaaatcgc ctcattcggg
    26101 cattcgggtt cacacatatc acaattaata cagcgtttag taattagtaa agacatttca
    26161 atggattacc gttaaatcat tttaaaatca gtaagttgta tcgagtttgt atgctttact
    26221 gtcattaact tactgtatgt tgatccagtg tatttaacct tgataaactc agtccagcaa
    26281 cacaaaaccg caacacattg cattttgtcc cgtagaaaag acttgtatgt gtgagcttgt
    26341 tttctgcgcc tacgcagata aggattgaga atgccgcgca ctgtaacaca taatccggat
    26401 agccccaata atgacgatgt tttagccgca tctgaaaaat gggacgcctg taaacccccc
    26461 tataccagcg cacacatgaa aatctgtgtt gctgccgcca aaatcatcct cgctgcttcc
    26521 ggcgtggctc gctgttccaa atacgaaaaa gagaactatc tccgtatcga tttcagcaaa
    26581 gccggtaagg ttacatttta cgccgagttt ccaaaaaaga tgggcctcaa aggtaaaaag
    26641 ctcggcgagt ggccggagct cgttatccag ctggcgcgcg aaaaagcact aggtatggct
    26701 gacggtggac tgcgggcaga gtccgtacat gcagcgctgg aaatgtaccg ggatgaccca
    26761 aagccaaagt agcccggcag aagctgagcc cggacagttt cacaacctac ggggtgcgta
    26821 tcgaccggat taaagtaacg ttcggcgagc gtgaggtgtt cagcgacgta acatacaatc
    26881 ggctggtgga agtgctggac gagtggatcg ccactcgctc gaacaataac gccctggagt
    26941 tgtttgccga gctccgtcgg ttctggaagt tctgcgcacc tactctttgc aacggccgca
    27001 atgttgccgc cagtctgcca gatgattatg tttcctcccg cgtacagaaa cctaccccca
    27061 cacggctttt taccgatatt gaatcaatcg cccgactctg gctcaatgtt gctgcctgca
    27121 cctctgtaca ccagaagaat gctgttcgct tcatgatcat cactggtgtt cgtccgatta
    27181 atgtccataa cctgcgctgg gactacgttt acgaggaggc tggtgaaatt gtttatccgg
    27241 aaggggttat cggcatgcga ggggctatga aaacacaaaa ggctttccgc ctgccgataa
    27301 cgcctgagat ccggcggatt atcgacgagc agaaagcctg gcgtgattca gttcctgagt
    27361 gcaacaggga ttatgtattt ttgcagccac gtgatccaat gcagccattt tcaaaacgat
    27421 cactggataa gctggtgaaa acatacagcc cggacggggc tgta
//
LOCUS       DAJTSI010000048        27205 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000048 DAJTSI010000000
VERSION     DAJTSI010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27205
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.048"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..159)
                     /locus_tag="OO327_001770"
     CDS             complement(<1..159)
                     /locus_tag="OO327_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="YghU; B2989; one of eight putative glutathione
                     transferase proteins from E. coli; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR6018340.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTP"
     gene            364..2223
                     /gene="gss"
                     /locus_tag="OO327_001771"
     CDS             364..2223
                     /gene="gss"
                     /locus_tag="OO327_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR6018341.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2515..4014
                     /gene="pitB"
                     /locus_tag="OO327_001772"
     CDS             2515..4014
                     /gene="pitB"
                     /locus_tag="OO327_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCR6018342.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(4063..4755)
                     /locus_tag="OO327_001773"
     CDS             complement(4063..4755)
                     /locus_tag="OO327_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR6018343.1"
                     /translation="MQSITPPLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            4929..5642
                     /locus_tag="OO327_001774"
     CDS             4929..5642
                     /locus_tag="OO327_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR6018344.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            5674..6432
                     /locus_tag="OO327_001775"
     CDS             5674..6432
                     /locus_tag="OO327_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018345.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            6478..7827
                     /locus_tag="OO327_001776"
     CDS             6478..7827
                     /locus_tag="OO327_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCR6018346.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFVGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            7827..8651
                     /locus_tag="OO327_001777"
     CDS             7827..8651
                     /locus_tag="OO327_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018347.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            8663..9223
                     /locus_tag="OO327_001778"
     CDS             8663..9223
                     /locus_tag="OO327_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR6018348.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            9254..10324
                     /gene="lptF"
                     /locus_tag="OO327_001779"
     CDS             9254..10324
                     /gene="lptF"
                     /locus_tag="OO327_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR6018349.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            10321..11400
                     /gene="lptG"
                     /locus_tag="OO327_001780"
     CDS             10321..11400
                     /gene="lptG"
                     /locus_tag="OO327_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR6018350.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(11436..12607)
                     /locus_tag="OO327_001781"
                     /pseudo
     CDS             complement(11436..12607)
                     /locus_tag="OO327_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(12607..12855)
                     /locus_tag="OO327_001782"
     CDS             complement(12607..12855)
                     /locus_tag="OO327_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6018351.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(12888..13802)
                     /locus_tag="OO327_001783"
     CDS             complement(12888..13802)
                     /locus_tag="OO327_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR6018352.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(13799..15529)
                     /locus_tag="OO327_001784"
     CDS             complement(13799..15529)
                     /locus_tag="OO327_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HCR6018353.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQREFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            15891..16987
                     /locus_tag="OO327_001785"
                     /pseudo
     CDS             15891..16987
                     /locus_tag="OO327_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            complement(17111..17881)
                     /gene="glcC"
                     /locus_tag="OO327_001786"
     CDS             complement(17111..17881)
                     /gene="glcC"
                     /locus_tag="OO327_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HCR6018354.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            18132..19631
                     /gene="glcD"
                     /locus_tag="OO327_001787"
                     /pseudo
     CDS             18132..19631
                     /gene="glcD"
                     /locus_tag="OO327_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            19631..20683
                     /gene="glcE"
                     /locus_tag="OO327_001788"
     CDS             19631..20683
                     /gene="glcE"
                     /locus_tag="OO327_001788"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCR6018355.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            20694..21913
                     /gene="glcF"
                     /locus_tag="OO327_001789"
                     /pseudo
     CDS             20694..21913
                     /gene="glcF"
                     /locus_tag="OO327_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            21918..22322
                     /locus_tag="OO327_001790"
     CDS             21918..22322
                     /locus_tag="OO327_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HCR6018356.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            22344..24515
                     /gene="glcB"
                     /locus_tag="OO327_001791"
     CDS             22344..24515
                     /gene="glcB"
                     /locus_tag="OO327_001791"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCR6018357.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            24870..26456
                     /locus_tag="OO327_001792"
     CDS             24870..26456
                     /locus_tag="OO327_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HCR6018358.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVITLLQAYVFTGMLVS"
BASE COUNT         6545 a         6592 c         7258 g         6810 t
ORIGIN      
        1 cggcgttccc agcgaataaa gttgcaatgg atgtttgcca acgggcagcg ttttttcatg
       61 cgtcggacca gaaaacgggc gattgatatt ggcgaacgcg ccgccagcgg atttatccca
      121 cgtccagact ttcgcgggct gataagtatt gtctgtcata gtgagttgcc ttctgagtgg
      181 tagtgttgaa gcagtgtagc aggtcacttg ctgtacagaa tgtgaatttt ttaacccttt
      241 gcgccagccg ctgcgatgag gtgtatgatt agtctgacct ctgtccggtt aatgtcgctg
      301 agcaggtaat cagagcacaa caattacgcc aactgcttag gttgtattgt taaaggtaaa
      361 gtgatgagca aaggaacgac cagccaggat gccccgttcg ggacattatt gggctacgcc
      421 ccaggtgggg tagcaatcta ctcttcagat tacagttctc tcgatccgca ggaatacgaa
      481 gatgacgccg tattccgtag ctatatcgac gacgaatata tgggccacaa gtggcaatgc
      541 gttgaatttg ctcgccgttt tctctttctg aattacggtg tggtctttac tgacgtgggt
      601 atggcgtggg agattttctc gctgcgcttc ctgcgagaag tggttaatga caacatcctg
      661 ccattgcagg catttcctaa cggctcgccg cgtgcgccgg tcgcgggtgc gcttcttatc
      721 tgggataaag gcggtgaatt taaagacact ggccatgtcg ccatcattac ccaattgcat
      781 ggcaacaaag tccgtattgc ggaacagaac gtgattcatt ccccgttgcc gcaagggcaa
      841 cagtggacgc gcgagctgga gatggtggtc gaaaacggct gctataccct taaagacact
      901 tttgatgaca ccaccattct gggctggatg atccagacgg aagatactga atacagctta
      961 ccgcagccgg aaattgcagg cgagctgctg aaaatcagtg gagcgcgtct ggaaaacaaa
     1021 ggccagtttg acggtaaatg gctggatgaa aaagatccgc tacaaaacgc ctatgtgcag
     1081 gccaacggtc aggtgatcaa tcaggatccg tatcattact acaccattac cgagagtgcc
     1141 gagcaggagc taattaaagc caccaacgag ctgcatctga tgtatcttca cgcaaccgac
     1201 aaggtgctga aagatgacaa cctgctggcg ctgttcgaca ttccgaaaat cctctggcca
     1261 cgtttgcgtc tctcctggca gcgtcgccgt caccatatga tcactggtcg tatggatttc
     1321 tgcatggatg agcgtggcct gaaggtttac gaatacaacg ccgactccgc ctcctgtcat
     1381 accgaagcgg gcttgatcct cgaacgttgg gcggagcagg gctataaagg caacggcttc
     1441 aatccggcgg aagggctgat taacgaactg gctggtgcct ggaaacacag tcgtgcacgt
     1501 ccgtttgtcc atatcatgca ggacaaagat atcgaggaaa actatcacgc gcagtttatg
     1561 gagcaggcgc tgcaccaggc gggctttgaa acgcgtatct tgcgcgggct ggatgaactg
     1621 ggctgggatg ctgccgggca actgattgat ggggaagggc gactggttaa ctgcgtgtgg
     1681 aaaacctggg cgtgggaaac cgcgtttgat cagattcgtg aagttagcga ccgtgagttt
     1741 gctgcggtgc caatccgtac cggtcatccg caaaacgaag tgcgtcttat cgacgtattg
     1801 ctgcgcccgg aagtgctggt ctttgagccg ctgtggacgg tgatccccgg caacaaagcg
     1861 attctgccga tcctctggtc gctgttcccg caccatcgtt acctgctgga taccgatttc
     1921 actgttaatg atgaattggt gaaaacaggt tacgcagtga aaccgatcgc cggtcgctgt
     1981 ggtagcaata tcgacctggt cagccatcat gaagaggtgc tggacaaaac cagcggtaaa
     2041 tttgccgagc agaaaaacat ctatcagcaa ctgtggtgtt tgccgaaagt ggacggtaaa
     2101 tacattcagg tatgtacctt caccgttggc ggcaactacg gtgggacgtg tttgcgcggt
     2161 gatgaatcac tggtcatcaa aaaagagagt gatattgaac cgttaattgt ggtgaaaaag
     2221 taatacatgt tgtagcaacc ccgtactgat tatgaagaat aattagtacg gggattaatc
     2281 aaaatattca atatttatat attccaatat ttattcattt catatgtgaa tatattaacc
     2341 agtggaatac ctgtgttttg attatttcta aaggttttga atgaatgctt attgtctgat
     2401 acacaagaaa taacactctt tttatcgtta aaaaatgata tttcactttg cccatgccgt
     2461 taaagtcact gataatgcgt ccgttcgtaa attcaaaatg gcgtaatcta atatatgcta
     2521 aatttatttg ttggccttga tatatatacg gggcttttgt tattacttgc tctggcattt
     2581 gtgttgttct acgaagcaat caatggtttt catgacacgg cgaatgcggt ggcaaccgtt
     2641 atttatactc gtgccatgca gccacaactt gctgtggtga tggcggcatt ttttaacttt
     2701 tttggcgtgt tattgggcgg acttagcgtt gcctatgcca ttgtccatat gttaccaatc
     2761 gatttgttgc tgaatatggg gtcaactcac ggtttggcga tggtcttttc catgctgctg
     2821 gcggcgatta tctggaacct gggaacgtgg ttctttggtt taccggcctc cagttcgcac
     2881 accttgattg gtgcgattat cggcatcggt ttaaccaacg cgctgttaac cggctcatcg
     2941 gtgatggatg cgttaaacct gcgtgaagta accaaaattt tctcctcgct gattgtttcc
     3001 cctatcgtcg gcctggtcat tgcgggtggt ctgattttcc tgctgcgccg tttctggagt
     3061 gggacgaaaa agcgtgaccg tattcaccgc attccggaag atcgcaaaaa gaaaaagggc
     3121 aaacgtaaac cgccattctg gacgcgtatt gcgctgattg tttccgctgc gggcgtggcg
     3181 ttttcgcacg gcgcgaacga cgggcagaaa gggatcggcc tggtaatgct ggtactggtg
     3241 gggattgccc ctgctggctt cgtcgtcaat atgaatgcgt ccggctatga aattacccgt
     3301 acccgcgatg ccgttaccaa cttcgaacac tacctgcaac agcatcctga actgccgcag
     3361 aagttgattg cgatggaacc tccattgcct gcagcatcga ctgatggcac gcaagtaaca
     3421 gagtttcact gtcatccggc aaataccttt gatgcgattg cgcgcgttaa aacgatgctg
     3481 ccaggcaata tggaaagtta cgagccgtta agcgtgagtc agcgcagcca gctgcgccgc
     3541 attatgctgt gcatctccga tacctccgcg aagctggcga aactgccagg cgtcagtaaa
     3601 gaagaccaga acctgctgaa aaaactgcgc agcgatatgt taagcaccat tgagtacgct
     3661 ccggtgtgga tcatcatggc ggtagcactg gcgctcggca ttggcaccat gattggctgg
     3721 cgtcgtgtag cgatgaccat cggtgagaag attggtaagc gcggcatgac gtatgcgcaa
     3781 ggcatggcgg cacaaatgac ggcggcagtg tctatcggcc ttgccagtta tattgggatg
     3841 cctgtctcca caacacacgt cctctcgtct gcggtagcag ggacgatggt ggtggacggc
     3901 ggtgggttac agcgtaaaac ggtaaccagc atcctgatgg cgtgggtgtt tactttaccg
     3961 gcggcaattt ttctttctgg tgggctgtac tggatagcat tgcagttgat ttaaccttcc
     4021 tgaaaatgcc cggtcctggc gatcgggcat ttccattttt gactagtgat aaccacgcgc
     4081 ggtcataaaa tccgtaatcg ctttttctgc atcaaccaac acctgttcca gtggctgatt
     4141 ggcatcgata tcaaccagtt gtgcaccacc aaaggttaac tgtggcgtta tggcaatctt
     4201 cctcgccagc gattcccgtt tatggtcggg tttacgtgca caggcaactt caaggtcaac
     4261 attgagtttg atgaccagat caggcttatg gctcgccatc cagtgaaacg ctttacgttc
     4321 ctggcttgcc agccatgaga caaaacgacc accttcaacg ttaggtggga acaccgtacc
     4381 atcgtaagcg ccaggaattt ggtcctgagg ataacggtcg gttagaacaa ttaacccgcg
     4441 acgacgacag gcaagcatat gacgaaagcg cagtaagcga cgggcgacaa acgctgtaat
     4501 taccagcgcc ggaactggtc caggcaattt ttttgctgtt ttcacctgat ttcgttcaat
     4561 tgttttatgt aaggattttc ccatcaaagg taattttgtc actgcacgac cgacatttcc
     4621 ggcctgtttt cctaaatgaa ctctttcggc agcaccatat ttttcgacaa cggtaataag
     4681 atgttcacac accgttgact tgcctgaagc atcgctacca ataacggcaa ttaatggagg
     4741 tgttattgac tgcatgaata tatcctttaa tttaaatatc cattaaaaat atttatttgg
     4801 ttaatatgtt tttatgaaag cgtaattcag gtcaatgtca caattagtca ttgtcacaat
     4861 aaggttgaac ggatattctg agcacggacc acgtaatatt caatacatta tttactgccg
     4921 ggtttttcgt gaatcaacgt aatatgtcaa taattaattc cacgccagtg cgtgttattg
     4981 ccattgtagg atgtgatggt tcaggtaaat cgaccctcac ggcaagcctg gtaaatgaac
     5041 tggcagcaag aatgccaaca gaacacattt atctcgggca atcgtccggg cgaattggcg
     5101 aatggatttc acagctccct gttattggcg caccttgtgg gtgttatctg cgaagtaaag
     5161 cggcacatgt acacgaaaag cccttaactc cgcctggcaa tattactgcg ctggttatct
     5221 atctgctttc ctgctggcgg gcgtacaagt ttcgcaaaat gttgtgtaaa agccagcaag
     5281 gctatctgct catcaccgac cgctacccgc aggttgaagt gccgggtttt cgctttgatg
     5341 gcccgcaatt ggcaaaaacc acgggcggta acggttggat aaaaatgtta aggcagcgcg
     5401 agttgaagct gtaccaatgg atggcgtctt atttacccgt attgttgatt cgtcttggca
     5461 ttgatgaaca aaccgcgttt gcgcgtaaac ctgaccacca actggcagcg ttacaggaaa
     5521 aaatcgccgt tacgccgcaa ctgacattca atggcgccaa gatccttgaa ctggatggtc
     5581 ggcaaccagc cgatgaaatt atgcaagcgt cactacgcgc aattcacgcc gcgctttcct
     5641 gatcaccaaa atggataggg gatgatttaa cgaatggatg cactacaaac acaaactgtt
     5701 aatagtacaa ccgcgccgca gcccaactac attccggggc tgattgcggt ggtcgggtgt
     5761 gatggcaccg gtaaatccac gctgaccacc gacctggtga aatcgctgca acaacactgg
     5821 caaaccgagc gccgctatct ggggctgctc tccggcgaag acggcgacaa aatcaaacga
     5881 ttgccgttgg ttggcgtctg gctggaacgg cgattggcgg ccaaatcctc gaaaacccaa
     5941 agcatgaaaa ccaaatctcc ggcgctatgg gcggcggtga ttatgtactg cttctcgctg
     6001 cgaagaatgg cgaatctacg caaggttcag cgactggcgc aaagtggcgt tctggtggtc
     6061 agcgatcgct tcccgcaggc tgaaatttcg ggcttttatt atgacggacc ggggatcggc
     6121 gtcgaacgtg cgaccgggaa aatcagcatg tttctggcgc agcgcgaacg gagtttatac
     6181 caaaaaatgg cgcaataccg tcctgaatta atcattcgtt tggggattga tattgatact
     6241 gccatctccc gcaagcctga ccatgagtat gccgagctgc aggacaaaat cggcgtcatg
     6301 tcgacgattg gctataacgg cacaaaaatt cttgagattg attccagagc gccctacagc
     6361 gaagtgctgg aacaggtaca gaaggcggtt tctctggttg ccatcgtttc tgaccgccga
     6421 agtttaactt aaggctggcg ggatattttt tactatcaac gataaggtca ggctgaattg
     6481 gcgggtttta acatcaaaca ttggtttgca gatggcgcgt ttcgcaccat tattcgcaat
     6541 agcgcctggt taggctccag taatgtcgag agcgccttgc tggggctgtt ggcgctctcg
     6601 tgtgccggta aagggatgac gcccgccatg tttggtgtac tggtgattgt gcaatcgtac
     6661 gccaagtcga tcagcgattt tattaagttt cagacatggc agctggtggt tcagtacgga
     6721 acgccagcct tgaccaacaa taatccgcag caatttcgca atgtcgtctc attttccttc
     6781 tcgctggata tcgtcagcgg cgcggtggcg attgtcggcg gcattgcctt actgccattc
     6841 ctttcccatt cattaggtct ggatgaccaa agcttttggc tggcggcgct gtattgcacg
     6901 ctcattcctt caatggcttc ctccacgccg accggcattc tgcgtgcggt agatcgcttt
     6961 gatttgattg ctgtacagca ggcgacgaaa ccttttctgc gcgcagcggg gagcgtcgca
     7021 gcctggtatt ttgactttgg ttttgtgggt tttgttattg cctggtacgt gtcgaatctg
     7081 gttggcggca ccatgtactg gtggtttgcc gcgcgcgaat tacgccgtag aaatatccat
     7141 aacgccttca aattgaatct gtttgagtct gcccgacaca ttaaaggcgc gtggagtttt
     7201 gtctggtcaa ccaacattgc ccactccatc tggtcggcgc gtaactcgtg cagcacagtg
     7261 ttggtgggga tcgtgttagg acccgctgcc gccgggttat ttaaaatcgc catgacattc
     7321 ttcgacgccg ccggaacgcc agcgggtttg ctgggtaaaa gtttttaccc agaggtgatg
     7381 cgtttagatc cgcgcaccac cagaccgtgg ttgttaggtg tgaagtccgg tttactggcg
     7441 ggcggaatcg gtattctggt ggcgctcgcg gtgttgatcg tcggcaagcc gctcatttcg
     7501 ctggtgtttg gcgttaagta tctcgaagcg tatgacctga ttcaggtgat gctgggcgca
     7561 attgtgattt cgatgctggg cttcccgcag gagtcattgc tattgatggc gggaaaacag
     7621 cgcgcttttc tcgtggcgca aaccatcgcc tcaatcggct acatcgtatt gctgttcatg
     7681 ttctgtcatc tgtttggtgt gctgggagca gcgtttgctt acttcggtgg gcaatgtctg
     7741 gacgtggcgc tctcgttaat tccaacctta aaggcatttt ttcagcgcca ttccttgctt
     7801 tataacgcag ccggagagaa atcctgatga tgagaaaata tttcccgctt gaggcgagcg
     7861 agcgtctatt tgttgcgatt gaagaagatg atgtcgttga tgcacaggtt agtttgccgc
     7921 cgactattgc actaagttgt accaccgaaa tcattcatga taattacgcg ttatgcctgc
     7981 aattctggct caatggcgtt gatcgtcagg aattactgcg tttagttcgc aaacaagcca
     8041 aaggcgatga gctgactgct gatgagcgca aacaatttaa atacatgcga gcgcgctaca
     8101 agcacctgcg ttttgcgcaa cggctgtatt taaagaagca tcaggccgga tttttattcg
     8161 gcaaaacgac cgtttttctg gggcgttttc aggacggctt tcgtaacggt aagaaaaata
     8221 ttgtgtcgta ttacggcaac ctgttacgga tttatttaag ctctccggtc tggtcgttgg
     8281 tgaactattc attacgccat agccagttag aaagcgtaag cagttttatt gcctatcggc
     8341 aaaagcaaat gcataccctg aaagagatta ttgccaaacc gcgactaacc ggcagggaat
     8401 ttcatgatgt gcgaaaaatc atcagccagc aggtttctta ttacgacacc ttaagatcgc
     8461 ttgatcctga aaataaagag gcgttacaga tttcccgttt cctcgccgcc attaatggcc
     8521 tgatgggaga taaacacgac gacatggtgg cggatgatat ggaaaaccgc cagtcgtatt
     8581 atgcaccgtt ggcgctggat agcgatattt gtcagcgctt agagctgctg atttcgcgtt
     8641 ttccgctata gccggagcaa tgatgtcatc tcgtttgatt attgcgttga ttatcatgct
     8701 cttagccccg ggtgtgcagg cacacaattt cgtcactggc aaaacggtta cccctgttta
     8761 tatccaggaa ggcggcgagc tgcttctgaa tagcgacgac gaaattcact accaaaagtg
     8821 gaacagcaca caattagcgg gaaaagtccg cattattcag tatatcgccg ggcgtaagtc
     8881 ggcgaagaaa aagaactcac tgttaattaa agcggttgaa gcggcaaact ttccgcagga
     8941 tcgttttcag cccaccacca ttgtgaacac cgatgatgcc atctttggta ctggctattt
     9001 tgttgttggc aaaatcgaaa aaaataaacg ccgctatccg tgggcgcaat tcgttattga
     9061 cggcaatgga cagggacgcg ttgcctggcg cttgccggag cagagttcaa ccattctggt
     9121 gctgaataaa gccgggcaaa ttcagtgggc aaaggacggc agcctgacgc cggaagaggt
     9181 agatcatgtc attgcgttgg cgcagaaact gatcaatgaa taaaaaactc aaaggattga
     9241 gggcgttagt gagatgaagc tggttgaaca ctacatcatg cgtggtacgc gccgtctggt
     9301 gctgattatc gtcggttttc tgatctttat tttcgccagc tactccgcac agcgttatct
     9361 gaccgaagcg gcaaatggga cattagctct tgatgttgtg ctggatatcg ttttctacaa
     9421 agtactgatt gcactggaga tgttgttacc tgttggtctg tatgtgtcag ttggcgtgac
     9481 gctagggcag atgtataccg actcggaaat taccgctatc tctgcggcgg ggggcagtcc
     9541 gggacgcttg tacaaagccg ttctttatct ggcgataccg ctaagtattt ttgtcaccct
     9601 tctgtcgatg tatggtcgtc cgtgggctta tgcgcagatt tatcaactgg agcagcagtc
     9661 gcagtcggag ctggatgttc gccagttgcg ggcaaagaaa tttaacacta acgataacgg
     9721 acgaatgatc ctttcgcaga cggttgatca ggataataat cgcctgactg acgcgctgat
     9781 ttatacttct actgccaatc gaacccgtat tttccgcgcc ggttcggttg atgtggttga
     9841 cccatcaccc gagaaaccga ccgttatgtt gcacaacggc actgcctatc ttctcgatca
     9901 tcaggggcgt gacgacaacg aacagatcta ccgtaatctg caattacatc tgaatccgct
     9961 ggatcaaagc ccgaacgtca aacgcaaagc gaaatcggtc acggagctgg cgcgctccgc
    10021 ctttcctgcc gatcatgccg aactgcaatg gcgacaaagc cgtggcctga ccgcattgtt
    10081 gatggcgctg ctggccattt cattaagtcg ggtaaaaccg cggcaagggc gattttcaac
    10141 gttattgcca ctgacgttgc tgtttattgc cattttttat ggcggcgacg tctgccgtac
    10201 actggtggct aacggcgcga ttcccctcat tcctggtttg tggttagtgc ccggactcat
    10261 gctaatgggc ctgctgatac tggtcgcacg tgacttctct ttgctgcaga aattttcccg
    10321 atgaatgttt tcagtcgcta tttaatccgt catctctttc tcggttttgc cgccgctgca
    10381 gggctattgc tgccgctttt taccaccttc aacctgatta acgaactgga tggtgtcagc
    10441 ccgggcggtt atcgctggac tcaggcggtg ctggtggtgc taatgacctt accgcgcaca
    10501 ctggtcgaac tttcgccgtt tatcgcgtta ttgggtggta ttgttggact ggggcagtta
    10561 tcgaaaaaca gtgagcttac cgccattcgc agcacggggt tttctatctt ccgtattgca
    10621 ctggtggcgc tggttgccgg gatattgtgg actgtttcgt taggcgcgat agatgagtgg
    10681 gtggcgtcgc cattgcagca acaggcgttg caaatcaaat cgaccgccac cgcgttgggg
    10741 gaggacgatg acattaccgg caatatgctg tgggccaggc gtggtaatga atttgtgacg
    10801 gtgaaatcgc tgaacgagca gggccagccg gtgggcgtgg agatatttca ttatcgcgac
    10861 gatctctcgc ttgaatccta catttatgca cgcagtgcca ccattgaaga cgacaaaacg
    10921 tgggtcctgc atggtgtgaa tcataaaaaa tggctgaatg gcaaagaaac gctggaaaca
    10981 ttagataatc ttgcctggca atcggccttc accagtatga atcttgaaga gttatcaatg
    11041 ccggggaata ctttttctgt ccgtcagctt aatcattaca tccattattt gcaggaaacc
    11101 gggcaaccca gcagcgaata ccaccttgca ctgtgggaaa aactggggca acccatcctg
    11161 accctggcga tgattttgct ggctgtgccg tttaccttta gtgccccgcg ttcgcctggg
    11221 atgggtagcc gtctcgctgt aggcgtcatc gtgggcttac tcacctggat cagctatcaa
    11281 atcatggtta atctgggatt gctatttgcg ttaagcgcac ctgttaccgc gctcggttta
    11341 ccggtagcgt ttgtgttggt ggcgttgagc ctggtgtatt ggtatgacag acaacattaa
    11401 acccgccgtg gcgcaaacac cacggcgggc tcacattact gcttcagagt agcaaatgcc
    11461 tgaatgatgg catcgatttg ctcacgactg tgggcagcat ttacgctaca gcgcagcagc
    11521 gtgatgcctg caggcgcggc aggcggcagg ataagattca catacacgcc cggcgagata
    11581 agctcgcgcc agatgcgtaa accttcctct ttcgaaccga ttattactgg cactaccggg
    11641 ctaatatgtg aacttaactc ataacccagt ttttgcagac cgtcatacag atggttggcg
    11701 ttatccatca gcttttgccg cagctctggt tgtgtggcaa tagttcttaa cgacgagcgc
    11761 acggtcgcaa tgcaggacgg agaaggagag gcggtaaaaa tatacgggcg gctgccgtag
    11821 cgcagcactt ccatcgcttc cgaaacgaca gcaaaaccgc caatcgaggc cagactttta
    11881 ctgaaagtac caacgataat atccacatca tcttcaacgc cgacagcttc agccagtccg
    11941 cgccccgttg cgcctaacac gccaaacgaa tgcgcttcat cgacaatcaa ataaccaccg
    12001 agacgacgtt tgatatcaac aatttccgcc agcggcgcaa cgtcaccgag catgctgtag
    12061 atgccctcaa cgatgataat cgcctctttc gcccgttcac cgaggcgaac catgcgccgt
    12121 tccagatctt tagcatcgtt atggcggaaa cgaataattt ccgcgccacc gagagaacag
    12181 acgtcataaa tactggcgtg gctgtcggca tccagtaaca ccacggcgtt atggtcggca
    12241 agtgcgctga tgacgcctaa attagcggta taaccggttg aaaaacgata gcggtagggc
    12301 gattgaaaaa ctcggcaatc tctttctcta acgccaggtg cggcgcataa ctgccgtttg
    12361 ccatacgaga accggtggtg ccggtgcctt gggccgctaa cgcggcctga ccttcgctga
    12421 tggcatcatg attaaaggtt aaaccgagat aattattggt cccggccaga atcacttttt
    12481 tattgccgat ccggccttcg gtggcggaat acacttcatc aatacaggtg ccaaacggtg
    12541 ttaacccgtt gtcactaaat tgcagacgct cgccagcaag gcgcgcatat ttatcgtata
    12601 gccccattat ttattctcca gccatggaag tagagtttcc attaattgcg caggggtttt
    12661 gacatccagc agaatattaa taggaatgga aatatcgaat ctgtcttcca gcatcattaa
    12721 gagatccatc actttgatcg actccaggcc aagatcgttg acgagatcgc tgtcgggctt
    12781 aatttccaca ccgttttcga ccaaatcctg taaacaggaa aggatgtaat ccattactat
    12841 ttcacgattt accatagaaa cgttaacaca cctttaaaac aagacaatca aaatagcccg
    12901 ttgcgtaatg cgtgttccag gctaatcccg ggaaaccaat tgatatcttc agaaatacgg
    12961 ttattacttg ccgaccagtc ggcgtgggtt aattcacgaa ttttcgagcg ggtcagcata
    13021 ggttctttac ctgccaggcg actcaacgcg gtactgatat ccgcaaggcc tgtgagtacc
    13081 ggcagaggaa tgtcaaccat tcgcacggaa ccacaacggg ctttggcggc aagctgtcgt
    13141 acgcgttgcc agtcatagcc accggcgacg ccatcgcata attcataggt ttgcgtctgt
    13201 acagtttcgg cgcttaacca ctgacccact gcttgcgcaa aatcggtgac gtgaagaaaa
    13261 gagagttgtg tatctggcgc accaagtcgt ggcagcaggc cgcgtagcat ccagtcaaac
    13321 agcggtttta actctttatc gccgggacca tacacggctg tcgggcgaaa aacgcccagc
    13381 gtaatttcgt ccgccattgc tgccagtcgt tgctcggcga cgtatttgga ttttgcatac
    13441 caggagagct cgggatggcg cgccgccagc gaagagataa acagaaaacg ttggcaaaag
    13501 ccgctctctt ttgctgcttg catcaggcgc aggctgccgt caacgttaca gcgggtgaaa
    13561 atctcttctt tgtgcccgcg cacttgcccg gcgcaatgga ccactacgct ggctccggca
    13621 accagctcgc taagtgaatg agtatcttcc agcgaaccgc gcacccaggt gagattatca
    13681 ttgacgtgag cgcgggcagt acgcgtcaat gcgcgaacgt gaaagccgcg ggcgagcagg
    13741 ttatcgataa tatatttacc gataaacccg gtagcgcccg tcaccgcgac agtttgattt
    13801 atgccaggga ttcctgcaca ttaagactgg cagcataagc cttctgataa cgttttttcg
    13861 cttccgcacg ggcaggcttg ccggaggacg ttcggggaat actgtggggc ggcaacagat
    13921 cgatagccgc ggtcacgcca aattcgcgtt ggatccgtgc cgccagcgcg tggataagct
    13981 gcccgcgacg ttcttcgtcg ctgatccgac actggatctg caaaatgatt ttttcctggg
    14041 cggtaacaaa agcaatcgca tcgccagaat gaatttccgg ttcctgttcc gctatatatt
    14101 caatatcctg cggccagata ttacggccac gaataataat cagatcttta atgcgtccgg
    14161 tgacatacag ataaccgtcc agcagataac cgaggtcgcc ggtgtctaac cagcccgtcg
    14221 cggcaatctc gtcttgcgaa acctggtcgc caaagtaacc gctcatcaga ctgggaccgg
    14281 agatgcaaat atggcctacc acacgttccg cgaccggcat acctgcttca ttgcggattt
    14341 caataccatg ttccggcaac gctttgccgc agttgacgaa agtcgatacg gcgcgtgtct
    14401 ctgcacccgg cgcgacggct ttaccctgat attcgaggat gtcgcgatcc acttcgttaa
    14461 ccacaacccc ggaggcttca tcagagaagc tgacagccag cgcattttct gccagtccgt
    14521 agcacggcat gaaagtttta ttgtcaaagt taacctgacg gaaacattca gcgaattgat
    14581 ggagttgttc tgcggagatc ggctcagcac caataccagc gacgcgccag caggaaagat
    14641 ccagttcagc gagatctttt tcattcacgc ggcgttggca caattcatag ccaaacggcg
    14701 gcgcaacgga aacggtgccg cgatttttac tgatcaattt aagccattgc aaaggacgca
    14761 tggcaaaatc ctgagtgcgc aaataatcta ctgaaagttg cgtggcgacg gggggcagga
    14821 gaaagccgac cagtcccata tcatggtaga aaggctgcca ggagacgcag cggtcgctag
    14881 ggcgtaattt aatgccatcg tggcttatag cacgtagatt agccatcact tcgcgatggg
    14941 tgataatgac gccacgggga aaacgggtgc tgccggaggt gtactggagg taggcgatat
    15001 cgttcggaac tggacgctgg agcgcaacat cggcttccgg taatgcctta aaccaagcgt
    15061 ggcttaaaac atgtaattcg gggttgttat gcgtcgcggc attgaccagt ggcaaccact
    15121 catcaccagt gataatggct gcgggctggc aacttgccag taaaccctgg agtttggcgc
    15181 tccaggaatc ccgctgaccg acgcccattg gaatcgccaa cgggacggcg actaagccag
    15241 catactgaca ggcaaaaaaa gcctctacaa acccgctact cgtttcggca atcagcgcca
    15301 cgcgttcgcc ttttttcagg ttcagcgata acaaccgctt cgcaccagct tcggcacgcg
    15361 tttttaatgt ctgatattcc agttgatctt caagttggca acgtctgtca taaaaattca
    15421 ttccggctct actcagagcg gcgtagtcca aagcatcaac cagcgttgga aaatcggcat
    15481 agcgcatagg tagggaatgc gtaaagattt tattagacat atacaccacg aaaaaattgg
    15541 atatcaaaaa taaaatgaca ataatgcggt ggattatata ggcataaaag ataaatgaga
    15601 tagtattttc atcggtgtga aaatgaacat tgatttaaat aatgagttta agttttttgt
    15661 ttttattatg aatgatattt aatttatgaa tatattaaga agaagccttg tccggtcgcc
    15721 ttaaattgac aaaaggcatt gaggcgtttc tgtgtagcct ctgaccttat cataatcatt
    15781 aattaatact atttattcgt cgtttgtatt cattgagttg attattaaga tgccgctcgt
    15841 tgctgttgca acctgttttt caccattaat tatttgttat ccacagagat atggaatgcg
    15901 atctgctgat gattaaaatt gaaaaagtaa ttaataaaaa tgaccttaag gcatttatcg
    15961 cttttccgtc atcactttat cccgacgatc caaactggat acccccttta ttcattgaac
    16021 gcaacgagca tttgtctgcg aaaaatccgg ggcagatagt tgggcgtatt accgcgcaaa
    16081 tagatacctt gcaccgtgag cgttacggtg aagataccgg tcatttcggc atgattgacg
    16141 ccattgatga tccgcaggat tttgccgcgt tgtttggcgc agcggaagcg tggttgaagt
    16201 cacaaggtgc aagtaagatc agcgggcctt tcagcctgaa tatcaatcag gaaagcggat
    16261 tactgattga aggttttgac acaccaccct gtgcgatgat gccacacggc aaaccgtggt
    16321 atgccgcgca tattgaacaa ctgggttatc acaaaggcat tgatttactg gcgtggtgga
    16381 tgcagcgaac cgatctcact ttctctccgg cgctaaaaaa actgatggat caggtgcgca
    16441 aaaaggtgac cattcgctgc atcaatcgtc agcggtttgc cgaagagatg cagatcctgc
    16501 gtgagatttt caactctggc tggcagcaca actggggatt tgtgccgttt accgaacatg
    16561 aattcgcgac catgggcgac caacttaaat atctggtgcc ggacgatatg atctacatcg
    16621 ctgagattga ttctgcaccc tgcgcgttta ttgtggcttg cctaacatca acgaggcgat
    16681 tgccgatctg aacggatcgc tctttccctt cggctgggca aaattgctgt ggcgtttgaa
    16741 agtcagcggt gtgcgaactg cgcgagtacc gctgatgggc gtgcgcgacg agtatcagtt
    16801 cagccgcatc ggcccggtga ttgcgctgtt attgattgaa gccttacgcg atccgtttgc
    16861 ccgccggaag atcgatgcgc tggagatgtc atggatcctc gaaaccaata caggcatgaa
    16921 taacatgctg gaacgcattg gggcggagcc gtacaagcgt taccgattgt atgaaaagca
    16981 aatttaatac cagatcctgt ttagcgcagg ggcggtcatt cgggagaggg tatagatgaa
    17041 cagatttaac accagatcct gttaagtgca ggggcggtcc cctcgccttg gggatagggt
    17101 aaaactctgc tcacattccc accttgttca tctccagcgg caccgccgag cgaatcaaat
    17161 ggtggccttc cagctcgata tcgtggagat tctttttcac ggtccgtaca tgatcgcgcg
    17221 ctgcgcgctg agcgacgtgc ggcagccgct gcaacaccgc gttgtagatc cgcgcatgct
    17281 ggcgatcgat ctgctttttt tgctgtggtc gatggtagag attatttacg ctggcaaaca
    17341 ctgaattaaa catcagatcg gtcaatgatt gcagcgtaaa caccagcacc tgattgtgag
    17401 aagcctgaca aatggcgaga tggaaagcgt gatccaactg cgcatgttcg atcagcgaaa
    17461 tctctttgtg gttctcactg gcggcgagca ttttttcata acagcgggtt atcacaacaa
    17521 aatcagcctg cgttcccagc gttgccgcca gccttgccga ttcgccctcc agtaatgcgc
    17581 gaacgtcgag cagatcgtac agcgttcgcg gctgcgtact gaacagatgg atcagcgggc
    17641 tggtgtcctg ctcccgatta agccgtgcga catgagaatc gcgaccctgc gccgtttcaa
    17701 taatcccgcg cccgcgcagc acggtcagcc cttcacgcag tgcggagcgt gagaagccga
    17761 gcttttcaca cagtcgacgt tccgagggaa gcggctgacc gaccttcagt acgccgtcga
    17821 taattaaccg ttcgatactc tctgcaacca cttcgcaaat agggcgacgt tcatctttca
    17881 ttcccggacc tcgtgcacag gtaggaccaa tttttctgtt cttaactcgc aaaacacgca
    17941 catcacgcaa gtgattatgt tacatcaatt taacattaag ttaaccaaga caaggtcaca
    18001 gagctggaaa aaaaatggtc tgaccggtag ctaaagagat agacgaaaac gaaaagcccg
    18061 cttaataact gttcacagaa gcagcgcgca aaaatcagct gccacacaac acaacaaagc
    18121 gaagcctact catgagcatc ttgtacgaag agcgtcttga tggcgcttta cccgatgtcg
    18181 accgcacatc ggtactgatg gcactgcgtg agcatgtccc tggacttgag atcctgcata
    18241 ccgatgagga gatcattcct tacgagtgtg acgggttgag cgcgtatcgc acgcgtccat
    18301 tactggttgt tctgcctaag caaatggaac aggtgacagc gattctggct gtctgccatc
    18361 gcctgcgtgt accggtggtg acccgtggtg caggcaccgg gctttctggt ggcgcgctgc
    18421 cgctggaaaa aggtgtgttg ttggtgatgg cgcgctttaa agagagcctc gacattaacc
    18481 ccgttggtcg ccgcgcgcgc gtgcagccgg gcgtatgtaa cctggcgatc tcctaggccg
    18541 ttgcgccgca taatctctat tacgcgccgg atccttcctc acaaatcgcc tgttcaattg
    18601 gcggcaatgt ggcggaaaat gccggtggcg tccactgcct gaaatatggt ctgaccgtac
    18661 ataacctgct gaaaattgaa gtgcaaacgc tggacggcga ggcgctgacg ctgggatcgg
    18721 acgcgctgga ttcacctggt tttgacctgc tggcgctgtt caccggatcg gaaggtatgc
    18781 tcggcgtgac caccgaagtg acggtaaaac tgctgccgaa gccgcccgtg gcgcgggttc
    18841 tgttagccag ctttgactcg gtagaaaaag ccggacttgc ggttggtgac atcatcgcca
    18901 atggcattat ccccggcggg ctggagatga tggataacct gtcgatccgc gcggcggaag
    18961 attttattca tgccggttat cccgtcgacg ccgaagcgat tttgttatgc gaactggacg
    19021 gcgtggagtc tgacgtacag gaagactgcg agcgggttaa ctacatcttg ttgaaagcgg
    19081 gcgcgactga cgtccgtttg gcacaggacg aagcagagcg cgtacgtttc tgggccggtc
    19141 gcaaaaatgc gttcccggcg gtaggacgta tctccccgga ttactactgc atggatggca
    19201 ccatcccgcg tcgcgccctg cctggcgtac tggaaggcat tgcccgttta tcgcagcaat
    19261 atgatttacg tgttgccaac gtctttcatg ccggagatgg caacatgcac ccgttaatcc
    19321 ttttcgatgc caacgaacct ggtgaatttg cccgcgcgga agagctgggc gggaagatcc
    19381 tcgaactctg cgttgaagtt ggcggcagca tcagtgggga acatggcatc gggcgagaaa
    19441 aaatcaatca aatgtgcgcc cagttcaaca gcgatgaaat cacgaccttc catgcggtca
    19501 aggcggcgtt tgaccccgat ggtttgctga accctgggaa aaacattccc acgctacacc
    19561 gctgtgctga atttggtgcc atgcatgtgc atcacggtca tttacctttt cctgaactgg
    19621 agcgcttttg atgctacgcg agtgtgatta cagtcaggcg ctgctggagc aggtgaatca
    19681 ggcaattagc gataaaacgc cgctggtgat tcagggcagc aatagcaaag cctttttagg
    19741 tcgcccagtc accgggcaaa cgctggatgt gcgttgtcat cgcggcattg ttaattacga
    19801 cccgaccgag ctggtgataa ccgctcgtgc cggaacgccg ctggtggcga ttgaagcggc
    19861 gctggagagc gccgggcaaa tgcttccctg cgagccgccg cattatggtg aagaagccac
    19921 ctggggcggg atggtcgcct gcgggctggc ggggccgcgt cgcccgtgga gcggttcggt
    19981 ccgcgatttt gtcctcggca cgcgcatcat taccggcact ggaaaacatc tgcgttttgg
    20041 tggcgaagtg atgaaaaacg ttgccggata cgatctctca cggttaatgg ccggaagcta
    20101 cggttgtctt ggcgtgctca ctgaaatctc aatgaaagtg ttaccgcgac cgcgtgcctc
    20161 tctgagcctg cgtcgggaaa tcagcctgca agaagccatg agtgaaatcg ccgagtggca
    20221 actccagcca ttacccatta gtggcttatg ttacttcgac aatgcgttgt ggatccgcct
    20281 tgagggcggc gaaggatcgg taaaagcagc gcgtgaactg ctgggtggcg aagaggttgt
    20341 gggtcagttc tggcagcaat tgcgtgaaca acaactgccg ttcttctcgt taccaggtac
    20401 cttatggcgc atttcattac ccagcgatgc gccgatgatg gatttacccg gcgagcaact
    20461 gatcgactgg ggcggggcgt tacgctggct gaaatcgaca gccgatgaca atcaaatcca
    20521 tcgcatcgcc cgcaacgctg gcggtcatgc gacccgcttt agtgccggag atggtggctt
    20581 tgccccgcta tcggctcctt tattccgcta tcaccagcag cttaaacagc agctcgaccc
    20641 ttgcggcgtg tttaaccccg gtcgcatgta cgcggaactt tgaggagcag gctatgcaaa
    20701 cccgattaac cgaagagatg cggcagaacg cgcgctggaa gccgacagca tcctgtgcgc
    20761 ctgtgttcac tgcggatttt gtaccgcaac ctgcccaacc tatcagcttc tgggcgatga
    20821 actggacggg ccgcgcgggc gcatctatct gattaaacag gtgctggaag gcaacgaagt
    20881 cacgcttaaa acacaggagc atctcgatcg ctgcctcact tgccgcaatt gtgaaaccac
    20941 ctgtccttct ggtgtgcgct atcacaattt gctggatatc gggcgtgata ttgtcgagca
    21001 gaaagtgaaa cgcccactgc cggagcgaat gctgcgcgaa ggattgcgcc aggtagtgcc
    21061 gcgtccggcg gtcttccgtg cgctgacgca ggtagggctg gtgctgcgac cgtttttacc
    21121 ggaacaggtc agagcaaaac tgcctgctga aacggtgaaa gctaaaccgc gtccgccgct
    21181 gcgccataag cgtcgggttt taatgctgga aggctgcgcc cagcctacgc tttcgcccaa
    21241 caccaacgcg gcaactgcgc gagtgctgga tcgtctgggg atcagcgtca tgtcagctaa
    21301 cgaagcaggc tgttgtggcg cggtggacta tcatcttaat gcgcaggaga aagggttggc
    21361 acgggcgcgc aataatattg atgcctggtg gcccgcgatt gaagcaggtg ccgaggcaat
    21421 tttgcaaacc gccagcggtt gcggcgcgtt cgtcaaagag tatgggcaga tgctgaaaaa
    21481 cgatgcgtta tatgccgata aagcgcgtca ggtcagtgaa ctggcggtcg atttagtcga
    21541 acttctgcgc gaggaaccgc tggaaaaact ggcaattcgc ggcgataaaa aactggcctt
    21601 ccactgcccg tgtaccctac aacatgcgca aaagctgaac ggcgaagtgg aaaaagtgtt
    21661 acttcgtctt ggatttacct taacggacgt tcccgacagc catctgtgct gcggttcagc
    21721 gggaacatat gcgttaacgc atcccgatct ggcacgccag ctgcgggata acaaaatgaa
    21781 tgcgctggaa agcggcaaac cggaaatgat cgtcaccgcc aacattggtt gccagacgca
    21841 tctggcgagc gccggtcgta cctctgtgcg tcactggatt gaaattgtag aacaagccct
    21901 tgaaaaggaa taaaaaaatg aaaactaaag tcattcttag ccagcaaatg gcgagtgcaa
    21961 ttattgccgc aggtcaggaa gaggcgcaga aaaataactg gtctgtttcc attgctgttg
    22021 ccgatgacgg cggtcatctg ctggcgttaa gtcgcatgga cgattgcgcg ccgattgcgg
    22081 cttacatctc ccaggagaaa gcgcgtaccg ccgcgctggg gcgtcgtgaa actaagggct
    22141 atgaagagat ggtgaacaac ggacgtaccg cgttcgtgac tgcgccgtta ctgacgtcgc
    22201 tggaaggcgg cgtaccggtt gttgttgatg ggcaaattat tggtgccgtg ggcgtttctg
    22261 gtttaaccgg agcacaggat gcgcaggtcg cgaaagtggc agcagcggtg ttggcgaaat
    22321 aagcgaaaac gaggagagaa acaatgagtc aaaccataac ccagggccgt ttacgcattg
    22381 acgccaattt taaacgtttt gtggatggag aagttttgcc gggcgtggaa ctggacgctg
    22441 ctgcattctg gcacaatgtt gatgagatcg ttcacgatct ggcaccagaa aatcgtcagt
    22501 tgctggcaga acgcgatcgc attcaggcgg cgcttgatga gtggcatcgc agcaatccgg
    22561 ggccggtaaa agataaagcg gcctataaat ctttccttcg tgaactgggc tacctggtgc
    22621 cgcaaccgga gcgcgtgacg gtggaaacca cgggcattga cagcgaaatc accagtcagg
    22681 cggggccaca gctggtggtt ccggcaatga acgcccgcta cgcgctgaac gcggcgaacg
    22741 ctcgctgggg ctcactgtac gatgcgttat acggcagcga catcatcccg caggaagggg
    22801 cgatggtcag tggctacgat ccgcagcgcg gtgagcaggt tatcgcctgg gttcgacgtt
    22861 tcctcgatga atcactaccg ctggaaaacg gcagctatca ggatgtggtg gcgtttaagg
    22921 tggtcgataa acaattacgc atccagttga aaaatggtaa agaaaccacg ttacgtactc
    22981 cagcacagtt tgtcggttac cgtggcgatg ccgctgcgcc gacctgcatt ttgctgaaaa
    23041 ataacggcct gcatattgaa ctgcaaattg atgccaacgg gcggattggc aaagacgatc
    23101 cggcgcacat caacgatgtt atcgtcgaag ctgctatcag taccattctc gactgcgaag
    23161 attcggtcgc ggcggttgat gcggaagata aaatcctgct gtaccgcaat ctgctgggcc
    23221 tgatgcaggg gactctgcaa gagaaaatgg agaaaaacgg tcggcaaatc gtacgtaaac
    23281 tgaatgacga tcgtcagtac accgccgccg atggctctga aatttctctg cacggacgct
    23341 cgttgctgtt tatccgcaac gtgggtcatt tgatgaccat tcctgtgatt tgggacagcg
    23401 aaggcaatga aatcccggaa ggtattcttg atggcgtcat gactggcgcg attgccctct
    23461 atgatttaaa agtgcagaaa aactcgcgca ctggcagcgt ctatattgtg aaaccgaaaa
    23521 tgcacggtcc gcaggaagtg gcgttcgcca acaaactgtt tacccgcatt gagacaatgc
    23581 tcggtatggc accgaatacc ctgaaaatgg gcattatgga tgaagaacgc cggacctcgc
    23641 tgaacttgcg tagctgtatc gctcaggcgc gcaaccgcgt ggcgttcatc aataccggtt
    23701 tcctcgaccg taccggcgat gaaatgcatt cggtgatgga agctggcccg atgctgcgta
    23761 aaaaccagat gaaatcgacg ccgtggatca aagcctacga acgtaataac gtgctttccg
    23821 gtctgttctg tgggctgcgc ggtaaagcgc aaatcggtaa aggcatgtgg gcaatgccgg
    23881 acctgatggc agacatgtac agccagaagg gcgaccaact gcgtgccggg gcaaacacag
    23941 cctgggttcc gtcaccaacc gctgctacgc tccatgcgct gcactaccac cataccaacg
    24001 tacagagcgt gcaagccaac attgcccaga ccgagttcag tgctgaattt gaaccgctgc
    24061 tggacgatct gctgactatt ccggtggctg aaaacgctaa ctggtcggcg caagagatcc
    24121 aacaagagct ggataacaac gtgcagggga ttctggggta cgtggtgcgt tgggtggagc
    24181 aggggattgg ttgttcaaaa gtgccggata ttcacaacgt ggcgctgatg gaagaccgcg
    24241 caacgctgcg tatctccagc cagcatatcg ccaactggtt acgtcacggt attctgacca
    24301 aagaacaggt gcaggcgtcg ctggagaata tggcgaaagt ggttgatcag caaaatgctg
    24361 gcgatccggc ttatcgtccg atggcgggga atttcgctaa ctcgtgtgct tttaaagctg
    24421 ccagcgattt aatcttcctc ggcgtgaaac agccaaacgg ttataccgaa ccgttattac
    24481 acgcctggcg tttacgcgaa aaagaaagtc attaatagcg tcaaaaaagc cccggcaagt
    24541 tttattgtcg gggctatgat agtaactgcg gtagtacatt tatcccggaa ataaccggag
    24601 cgattatcag ggtttaacaa tattaaatac ccgtagcgca ttactagcgg gcctaaaaca
    24661 acaatatgga catcttaacg atactctgcg cttgcgtgtg cggaacagaa acccttaccc
    24721 gatatacttg ttatgccaga ggtagcaatt gcgttagcgg cgtactgttg ctgtaaagta
    24781 taacgcctat attttacagt tcagtcctct tttgcttatt acaaccttaa gtatatcaag
    24841 catataaaga taataagaga ctgaacaata tggttacctg gacccaaatg tatatgccga
    24901 tgggaggact ggggctatcc gctctggtcg ccctgatccc gataatattc ttcttcgttg
    24961 cactcgcggt attacgtctg aaaggacatg ttgctggagc aataaccctt atattatcta
    25021 tcctgattgc gatattcgcc tttaaaatgc cgattgatat ggcatttgct gctgcgggct
    25081 atggctttat ttatggatta tggccaattg cgtggattat tgtcgcggcg gtgttcctgt
    25141 ataaattaac cgttgccagc gggcagttcg atattatccg cagctcggtt atctccatca
    25201 ccgacgatca gcgtttgcag gtgttactga ttggtttctc ctttggtgcg ttgctggaag
    25261 gagctgctgg ctttggtgcg acggtggcgt ttggtgcgtt gggcgtgccg attctggtcg
    25321 ccggtcaggt aacgggaatc gatccgttcc acattggcgc aatggcggga cgtcagttac
    25381 cgttcctgtc ggttcttgtg ccgttctggc tggtagcaat gatggacggc tggaaagggg
    25441 tgaaagagac gtggccagcg gcgctggttg ctgggggaag cttcgctgtc actcagttct
    25501 ttacctctaa ctatattggt ccggaactgc cggatattac ttcggcgctg gtgagtatcg
    25561 tctcactcgc tttattcctt aaagtctggc ggccgaaaaa taccgaaacg gcaatcagca
    25621 tggggcaatc cgcaggtgcg atggtggtaa ataagccatc ttctggcggt cccgtgcctt
    25681 cagaatatag tctggggcaa atcattcgtg cgtggtcacc gtttttaatc ttaacggtgc
    25741 tggtcaccat ctggaccatg aagccgttta aagcgttatt tgctccgggc ggcgcgtttt
    25801 attcactggt gattaatttc cagatccctc atttgcatca acaagtgttg aaagcggcac
    25861 ccattgtcgc ccaaccaacg ccaatggatg cggtgtttaa attcgaccct ctctcggctg
    25921 gcggcaccgc tatttttatt gcggcgatta tctctatctt catcctcggc gtgggcatca
    25981 agaaagggat tggcgtcttt gccgaaatgc taattagtct gaagtggccg atattgtcga
    26041 ttggcatggt gctggcattc gccttcgtca ccaactattc cggcatgtcc accacgctgg
    26101 cgctggtact ggcaggtaca ggcgtgatgt tcccgttttt ctcaccgttt ctcggctggc
    26161 tgggcgtatt ccttaccggg tcggacactt cttcaaacgc cctgtttggt tcactgcaat
    26221 cgaccacggc gcagcaaatc aacgtctctg acactctgct ggtggcagca aataccagcg
    26281 gcggcgtaac tggcaagatg atctccccgc aatctatcgc cgtggcctgc gccgcgacgg
    26341 gcatggtggg ccgagaatct gaactgttcc gctacaccgt gaagcacagt ctgatttttg
    26401 ccagcgttat caccctgctg caggcgtatg tgtttaccgg gatgttagtc tcgtaaatca
    26461 ataaagccgg atgaaggtaa atatcacccg gctttccatt tgtctgtcgt ttatctcgcc
    26521 agggaaccgt ttttacgatt ggcttcttta tacgtagtct ccgttttacg atgcgaattg
    26581 tttaatattt atacaacttg gcttctgtgc tggttataaa gtacccactt tcatgagatc
    26641 aattttagta tgttattaca ttgcttcatc tgttgctttc atgacgaatg gcgttttttc
    26701 tcttatttat atttttcact agaacaatga gtagatcaaa attcccaaaa ctcccaaaaa
    26761 cataagatta ttcgcaaagc tgattttgca gaataatgag aacttttaag atttgtcgag
    26821 gatcaatttt aagcaattta tctccacttg ttacataaaa gatgccgtta ggttaataag
    26881 ttaatcttgc tataacagtg ttcgttactg cctattaata aagatatttg ggatagggta
    26941 acgtggatga ggattcgaaa tcatccgtct tgcggaaagt gaaatgttgg cagatgttaa
    27001 tattatgtat gtattttaac tccaacaaca caccaatcat tttgttagaa ataagaaaat
    27061 ttatggttaa ataatttggt gattatgaag cattaaatcg taatgccaaa tattgttcgc
    27121 tttggagagt attattgcct gtttgtgtct gttgagcaga aaaatcatct gaatttgaat
    27181 acttttatta ggtgtgaata aatga
//
LOCUS       DAJTSI010000049        26652 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000049 DAJTSI010000000
VERSION     DAJTSI010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.049"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            27..152
                     /locus_tag="OO327_001793"
     ncRNA           27..152
                     /ncRNA_class="other"
                     /locus_tag="OO327_001793"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            272..613
                     /locus_tag="OO327_001794"
     CDS             272..613
                     /locus_tag="OO327_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR6018359.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(604..1530)
                     /locus_tag="OO327_001795"
     CDS             complement(604..1530)
                     /locus_tag="OO327_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6018360.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            1620..2618
                     /locus_tag="OO327_001796"
     CDS             1620..2618
                     /locus_tag="OO327_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR6018361.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2615..2833)
                     /locus_tag="OO327_001797"
     CDS             complement(2615..2833)
                     /locus_tag="OO327_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR6018362.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2835..4850)
                     /gene="ligA"
                     /locus_tag="OO327_001798"
     CDS             complement(2835..4850)
                     /gene="ligA"
                     /locus_tag="OO327_001798"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR6018363.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4921..5907)
                     /gene="zipA"
                     /locus_tag="OO327_001799"
     CDS             complement(4921..5907)
                     /gene="zipA"
                     /locus_tag="OO327_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR6018364.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            6137..6898
                     /gene="cysZ"
                     /locus_tag="OO327_001800"
     CDS             6137..6898
                     /gene="cysZ"
                     /locus_tag="OO327_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR6018365.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            7083..8054
                     /gene="cysK"
                     /locus_tag="OO327_001801"
     CDS             7083..8054
                     /gene="cysK"
                     /locus_tag="OO327_001801"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR6018366.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8438..8695
                     /gene="ptsH"
                     /locus_tag="OO327_001802"
     CDS             8438..8695
                     /gene="ptsH"
                     /locus_tag="OO327_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR6018367.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8740..10467
                     /gene="ptsI"
                     /locus_tag="OO327_001803"
     CDS             8740..10467
                     /gene="ptsI"
                     /locus_tag="OO327_001803"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR6018368.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10508..11017
                     /gene="crr"
                     /locus_tag="OO327_001804"
     CDS             10508..11017
                     /gene="crr"
                     /locus_tag="OO327_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR6018369.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(11059..11910)
                     /gene="pdxK"
                     /locus_tag="OO327_001805"
                     /gene_synonym="thiJ"
     CDS             complement(11059..11910)
                     /gene="pdxK"
                     /locus_tag="OO327_001805"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR6018370.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            12015..12389
                     /locus_tag="OO327_001806"
     CDS             12015..12389
                     /locus_tag="OO327_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR6018371.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            12422..13145
                     /locus_tag="OO327_001807"
                     /pseudo
     CDS             12422..13145
                     /locus_tag="OO327_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(13349..14260)
                     /gene="cysM"
                     /locus_tag="OO327_001808"
     CDS             complement(13349..14260)
                     /gene="cysM"
                     /locus_tag="OO327_001808"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR6018372.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(14394..15491)
                     /gene="cysA"
                     /locus_tag="OO327_001809"
     CDS             complement(14394..15491)
                     /gene="cysA"
                     /locus_tag="OO327_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR6018373.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(15481..16356)
                     /gene="cysW"
                     /locus_tag="OO327_001810"
     CDS             complement(15481..16356)
                     /gene="cysW"
                     /locus_tag="OO327_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR6018374.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(16356..17189)
                     /gene="cysT"
                     /locus_tag="OO327_001811"
     CDS             complement(16356..17189)
                     /gene="cysT"
                     /locus_tag="OO327_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR6018375.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(17189..18205)
                     /gene="cysP"
                     /locus_tag="OO327_001812"
     CDS             complement(17189..18205)
                     /gene="cysP"
                     /locus_tag="OO327_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR6018376.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(18509..19300)
                     /gene="ucpA"
                     /locus_tag="OO327_001813"
     CDS             complement(18509..19300)
                     /gene="ucpA"
                     /locus_tag="OO327_001813"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR6018377.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(19487..20287)
                     /gene="murR"
                     /locus_tag="OO327_001814"
     CDS             complement(19487..20287)
                     /gene="murR"
                     /locus_tag="OO327_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR6018378.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            20451..21347
                     /gene="murQ"
                     /locus_tag="OO327_001815"
     CDS             20451..21347
                     /gene="murQ"
                     /locus_tag="OO327_001815"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR6018379.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            21351..22775
                     /gene="murP"
                     /locus_tag="OO327_001816"
     CDS             21351..22775
                     /gene="murP"
                     /locus_tag="OO327_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCR6018380.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(22955..23854)
                     /gene="yfeX"
                     /locus_tag="OO327_001817"
     CDS             complement(22955..23854)
                     /gene="yfeX"
                     /locus_tag="OO327_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR6018381.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(23950..24525)
                     /locus_tag="OO327_001818"
     CDS             complement(23950..24525)
                     /locus_tag="OO327_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR6018382.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(24586..25035)
                     /locus_tag="OO327_001819"
     CDS             complement(24586..25035)
                     /locus_tag="OO327_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR6018383.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(25022..25447)
                     /locus_tag="OO327_001820"
     CDS             complement(25022..25447)
                     /locus_tag="OO327_001820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR6018384.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            25661..26530
                     /gene="amiA"
                     /locus_tag="OO327_001821"
     CDS             25661..26530
                     /gene="amiA"
                     /locus_tag="OO327_001821"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR6018385.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            26534..>26652
                     /locus_tag="OO327_001822"
     CDS             26534..>26652
                     /locus_tag="OO327_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HCR6018386.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREA"
BASE COUNT         6489 a         7161 c         6587 g         6415 t
ORIGIN      
        1 aaaaaagcgc cctaaaggcg cttttttgct atctgcgata ctcaaagatt cgaacctgca
       61 gcaggtttga gttgagcgca gcgaaacaac ggagccgctc gcggcgacgg cccgaagggc
      121 gagcgaagcg agtcatcctg cacgacccac caatgcaaaa aagcgcccta aaggcgcttt
      181 tttgctatct gcgatttgta agattgattt ttcttctcaa gttccttgtt cttctaccga
      241 tattacctcg tacagtgacg aggactgaac aatggccact atctcttcaa cttcaattcc
      301 ttccatacaa acacagagta gtaacagggc atcacaaggt agcgatgttg cttcacaaat
      361 cgcgcggata agtcagcaaa tcatcaaact cactcaacaa attaaagaga ttgtagacac
      421 tagcggcagt gctgaagaca agcaaaaaca ggccgaattg attcaacagc aaatcaccct
      481 actggaaaca caactggcgc agttgcagaa acagcaggca gaaaaggcgc aggagaaaga
      541 gcagcgtttg tctctgaatg tatcccttct aaatcctgtt gaaaaaacga ctcatatcga
      601 tatctatatc tgatacaacg gatccccttc ccgaccagcc attagcgcat tgccctggtc
      661 tcgtaccacg tcccataacg cttcagcggc ggtggaaagt gaacggttct tcctgcgcac
      721 cagcatcaac tggcgttcaa ccaccggcgt aatgcgctta accaccagtg ggctaccttc
      781 aggcaatggt agcgccagtg caggaagaat actgatgccg atgcctgcgg ctaccatcgg
      841 gaacagcgtc gccggatgtc caatctcctg cacaatattc gcctgaatac cattgcgcgc
      901 cagcgctgcg tcaatcagcg gtcggctgcc tgacgcgtaa tcctgcaaca ccaatttcgc
      961 tccctgtaat gcctgccagg gtacataatc ttccacggcc aaagcgctat cgcgatggca
     1021 aagcagaaag aaaggctcgg aaagaatcgc ttcacattgc agatcgccca ctgggcctgg
     1081 atcgatgacg atgccaaagt cgacatctcc ctggcgaata ctttccatca cccactgctg
     1141 cggacgatca tgcaggacaa actgaatatc tggatagcgg cgatggcttt cagcaatgca
     1201 ttgcggaata agatgcgcgg aaatggtctg gctggcagcg acccgaactt ttccgctcag
     1261 ttgttgcccc atacgcccgg tatcgcgcaa cgtgctgtta agttcatcca gcagtcgctc
     1321 aagacgcaaa gccaactgct gccctgcatc tgtaagcacc acttcacgcg tggttctgtc
     1381 cagcaggcga acaccagtat gattttccag ctccttcaca ctgtgactta ctgccgactg
     1441 gctcaagccg atacgctctc ctgcacgact aaaacttttc tcctgcgcta ctgtgacgaa
     1501 aacctttaat tgttttaaag aataattcat ctgttttctt catgaatgga tgcaataaat
     1561 caattttatt tctcaaactg gaagaagcac aatagaacca tcgatcatct ggagtcttta
     1621 tgaaactttt tcgtatcctc gatcctttca ccttaaccct gatcacggtg gtgttgctgg
     1681 cctctttctt tccggccaga ggcgatttcg tccccttctt tgaaaatctg accaccgcag
     1741 ctattgccct gctgttcttt atgcacggcg cgaagttgtc gcgtgaggcg attattgctg
     1801 gcggtggtca ctggcgactg catttgtggg taatgtgcag caccttcgtg ctgtttccga
     1861 ttctgggtgt actgtttgcc tggtggaaac cggtaaatgt cgacccgatg ctctactccg
     1921 gttttctcta cttgtgcatt ctcccggcta ccgtgcagtc tgcaatcgcc ttcacgtcaa
     1981 tggcgggcgg taacgtcgcg gcggcggttt gttctgcgtc agcatccagc ctgctgggga
     2041 ttttcctttc accattgctg gttggtctgg tgatgaatgt tcacggtgca aggggcagcc
     2101 ttgagcaggt cggtaaaatt atgctgcaac tgctgctgcc gtttgtgttg gggcatcttt
     2161 cccggccgtg gattggtgac tgggtgtcgc gcaataaaaa atggattgcg aaaactgacc
     2221 agacgtccat tctgttggtg gtttatacag cgttcagcga agccgtcgtt aatggtatct
     2281 ggcataaagt tggctgggga tcattgctgt ttatcgtggt ggtcagctgc gttcttctgg
     2341 ctatcgtgat tgtagttaac gtctttatgg cacgccgact gagcttcaat aaggcagatg
     2401 aaattactat cgtcttttgt ggttcgaaaa agagtctggc aaatggcatc ccgatggcaa
     2461 acattctgtt ccccacatcg gtgatcggta tgatggtgct gcccctgatg attttccatc
     2521 agatccaatt gatggtctgt gcggtgctgg cgcgtcgata caaacgccag accgaacagt
     2581 tacaggcgca gcaggaaagc agcgccgata aagcttaaag cggacgcttc aggggctgga
     2641 ccagttgcgt cagcccctcg gttttaatca gcagcgtgat ttgcattagc tcaccgagct
     2701 tccctgccgg gaactcatct ttgcgggcaa accacagcag atactcctcc ggcaggtcaa
     2761 ttaagcgacg ccctttgtat ttgccaaacg gcattatcgt attggctatt tcaatcagct
     2821 gctctttttc catctcagct acccagcaaa cgcagcattt ccgcttcgtc gatgacttca
     2881 atgcccagtt cctgcgcctt cgccagttta gatcctgcag cttcacccgc tatcaccaga
     2941 tcggttttct tcgacacgct gcccgcgact ttcgcgccca gttcgaccag tcgagcttta
     3001 gcgtcatcac gcgacatctg gcttaagctg cccgtaagca ccacggtttt accagcaaac
     3061 gggctgtcaa tctcttccgc gttgataacg atcggcgcag gccagtgaac accttccgcc
     3121 aacagctcgc tgatgacatt gcggttgctt tcttcggcaa agaagttgtg aacgtgggat
     3181 gcaacgacaa tgccaacatc aggcaccttt tgcagctctt caatcgaagc ggcttccagc
     3241 gcttccagcg tgccgaaata tgccgccaga cctgctgcgg tggcctcgcc gacttcacgg
     3301 atgccaagtg catagaggaa gcgagcaaag gtggtttctt tcgctttttc cagcgcgtta
     3361 accacgtttt gtgccaattt tggccccata cgctccagtc cggtcagttt gcctgcggtg
     3421 agtttgaaca gatctgccgg agtatggaca tattcttttt caaccagctg atcgatgatt
     3481 ttgtcgccca ttccgtcaac atccatcgca cggcgggaaa caaagtgttt cagcgactct
     3541 ttacgctgcg caccgcaaat caggccaccg gtacagcggg caaccgcttc accttccaca
     3601 cgctcaacgt cagaaccaca taccggacaa tgcgtcggga atacaacctc acgggtatct
     3661 tccgggcgtt cagaaagcac gacgttaacc acctgcggga tcacgtcgcc agcgcggcga
     3721 atcaccactt tatcgccaat gcgtaaacca agacgttcga tttcatccgc attgtgtaag
     3781 gttgcgttac tcaccagcac gccagcaaca tggacaggtt ccagacgcgc aacaggcgta
     3841 atcgcgccag tacgcccaac ctgaaactcg acgtcacgca caaaggtcat ctgctcctgc
     3901 gccgggaatt taaacgcaac cgcccagcgc ggggcacgcg cgacaaagcc aagctgctcc
     3961 tgctgttcca gtgagttgac cttaatcacc acgccgtcga tatcaaagcc cagcgtcggg
     4021 cggtcttctt ccactttgtg atagaacgcc agcacttctt ccgccgattc acaaagcgtt
     4081 acccgatcgc tgaccggcac cccccacttt ttaaattgca gtaaacggcc aagatgagta
     4141 tccggcagct cgccaccttc cagaacacca acgccatagc agaaaaaagt gagcggtcgc
     4201 ttcgctgtaa tacgcggatc aagctgacgc agtgaaccag ctgccgcatt acgtgggtta
     4261 gcaaaaactt tcccgcccgt gcgtcgcgca tcttcgttaa tcttttcgaa ccccgcctgc
     4321 ggcaggaaca cttcaccacg cacttccaga cgcgccggga tattctctcc gtgcagcttc
     4381 agcggaatgg cgcgaatagt acgcacatta gacgtgatat cttccccggt ggtgccatcg
     4441 ccacgggtcg cggcactgac taaaacgcca ttttcataca gaatactgac ggcaaggcca
     4501 tccagcttca gctcacagca ccaggtgact ttctcgttgt ttttcagacg gtcctgcaca
     4561 cgtttgttga aagcaagaaa gctttcttca tcaaaaacgt tatccagtga cagcattggt
     4621 acttcatggc gtatctggct gaaagctgcc agcggcgcag cacctacacg ttgggtaggc
     4681 gaatcaggcg taatcagttc tggatgtttg gtttccagct cgcgcagttc gcgcatcagc
     4741 ctgtcgtatt cagcgtcggg aatttccggc gcatccatca catgataaag atattcatga
     4801 tggcaaagcg tcgttcgcag ttctgtcagt tgttgttcga ttgattccat atcgcaccat
     4861 caatgctaaa aacccccgac aagcgggggt tcgaagagga gttaatttgc cttaagtgta
     4921 tcaggcgttg gcgtctttga cttcgcggat gatgtcctgg tactcgcgca atttctgcgg
     4981 agtcatcata cggcgctgat cgtcaagcac gacaccgccc acttcatcgg caatatgctg
     5041 cgcagattgc agcatcagct tgaagttctg cagctcgtca ccgtaagacg gtacctgcat
     5101 aaagatagtg acacccggag tagtgaaatc cttcatttca ggatcaaagg ttcccggttt
     5161 caccatattc gccaggctga ataacgccgg gccgctgcca tccgggctaa gatgacgatg
     5221 gtaaatattc atatcgccaa aaatgaagcc cgcttgttga atgctgttaa gaagcagttc
     5281 accgtttagc tcgctaccgt gatgcgccgc gacgttcatg ataatcaccg cttctttgcg
     5341 cttcggttta tccataactg gagccggttc cgctacaggc tcaggctgtg gtgccgctac
     5401 gggttctgca ggctggaaag cctgttgtgc cggttgcggt gctgaatgca caggctgcgg
     5461 cgctggcgcg acctgtggcg aaactggctg ctgcaacggc tgttcaggct gcggctgata
     5521 ggcaggctgc tgcaccggct gcgctggacg cggagcatgt tgcggcggta cctgcgcttc
     5581 aggcggctgc tggaccggtt gacgcggctg cgcagacgca taaggcggta ggtactggtg
     5641 ttgcggcgac ggacgagcag cctcatgctc ctgagcgtta gccggggcat gattcacgcg
     5701 gtgaacacga acctcaccaa cgccctcatc atcttcgaca tcctcgtcat aagaatcgtc
     5761 gtcacgtttt gacttcattc gttttaatgg ccgatcgcgg aacatagaag atcgttcttt
     5821 acggctggtc cagaaaccat gtaccagtaa agcgattatg gcgatcgcgc caacaatgat
     5881 taatatcaga cgcaaatcct gcatcattat attctctgtt gttctaacac cttgccacca
     5941 cggcaaacat ttactcacta agagtatttg ccgattacct caagtgcaag tgcactatta
     6001 actttcacag cacaaagata gatgaaatcg tgctttttgc tgttttttcg aacatatcct
     6061 aactgtccat tgcgcaatta cccggtaaaa tacgcagaat tttcctggga ttggtcaaaa
     6121 ggagctcatc ctgattatgg tttcatcatt cacatctgcc ccacgcagcg gtttttacta
     6181 ttttgcgcaa ggctggaagc tcgtctcgca acctgggatt cggcgtttcg ttattttacc
     6241 gctgctggtc aatattttgt tgatgggggg cgcattctgg tggctcttta cacagctcga
     6301 tgtctggatc ccgactttca tgagttacgt tccggactgg ctacaatggc tgagttatct
     6361 gttgtggcct ctggctgtca tctctgtgct gttagtgttt ggctatttct tctccacgat
     6421 tgctaactgg attgccgctc cgtttaacgg tttattggct gaacaactgg aagcacgatt
     6481 gactggcgct acaccgccag ataccgggat tttcggtatc atgaaagatg tgccgcgaat
     6541 catgaaacgc gaatggcaaa aatttgtctg gtatctgccg cgcgcaattg tattactaat
     6601 tctttacttc atccctggta ttgggcaaac cgtcgcgccg gtactgtggt tcctgtttag
     6661 cgcctggatg ttagccatcc agtattgcga ttaccccttc gataaccaca aagtgccgtt
     6721 taaagagatg cgcaccgccc tgcgcacacg caaaatcacc aatatgcagt ttggtgcttt
     6781 aaccagcctg tttacgatga tcccgctgct taatctgttc atcatgcccg ttgccgtttg
     6841 tggcgcgacg gcgatgtggg tcgattgcta tcgcgataaa cacgcgatgt ggcggtaaca
     6901 atctaccggt tattttgtaa accgtttgtg tgaaacaggg gtggcttatg ccgcccctta
     6961 ttccatcttg catgtcatta tttcccttct gtatatagat atgctaaatc cttacttccg
     7021 catattctct gagcgggtat gctacctgtt gtatcccaat ttcatacagt taaggacagg
     7081 ccatgagtaa gatttttgaa gataactcgc tgactatcgg tcacacgccg ctggttcgcc
     7141 tgaatcgcat cggtaacgga cgcattctgg cgaaggtgga atctcgtaac cccagcttca
     7201 gcgttaagtg ccgtatcggt gccaacatga tttgggatgc cgaaaagcgc ggcgtgctga
     7261 aaccaggcgt tgaactggtt gaaccgacca gcggtaatac cgggattgca ctggcctatg
     7321 tagctgccgc tcgcggttac aaactcaccc tgaccatgcc agaaaccatg agtattgaac
     7381 gccgcaagct gctgaaagcg ttaggtgcaa acctggtgct gacggaaggt gctaaaggca
     7441 tgaaaggcgc aatccaaaaa gcagaagaaa ttgtcgccag caatccagag aaatacctgc
     7501 tgctgcaaca attcagcaat ccggcaaacc ctgaaattca cgaaaaaacc accggtccgg
     7561 agatctggga agataccgac ggtcaggttg atgtatttat tgctggcgtt gggactggcg
     7621 gtacgctgac tggcgtcagc cgctacatta aaggcaccaa aggcaagacc gatcttatct
     7681 ctgtcgccgt tgagccaacc gattctccag ttatcgccca ggtgctggca ggtgaagaga
     7741 ttaaacctgg cccgcataaa attcagggta ttggcgctgg ttttatcccg gctaacctcg
     7801 atctcaagct ggtcgataaa gtcattggca taaccaatga agaagccatt tctaccgccc
     7861 gtcgtctaat ggaagaagaa ggtattcttg caggtatctc ttctggagca gctgttgccg
     7921 cagcgttgaa actacaagaa gatgaaagct ttaccaacaa gaatattgtg gttattctac
     7981 catcatcggg tgagcgttat ttaagcaccg cattgtttgc cgatctcttc actgagaaag
     8041 aattgcaaca gtaatgccag cttgttaaaa atgcgtaaaa aagcaccttt ttaggtgctt
     8101 ttttgtggcc tgcttcaaac tttcgcccct cctggcattg attcagcctg tcggaactgg
     8161 tatttaacca gactaattat tttgatgcgc gaaattaatc gttacaggaa aagccaaagc
     8221 tgaatcgatt ttatgatttg gttcaattct tcctttagcg gcataatgtt taatgacgta
     8281 cgaaacgtca gcggtcaaca cccgccagca atggactgta ttgcgctctt cgtgcgtcgc
     8341 gtctgttaaa aactggcgct aacaatacag gctaaagtcg aaccgccagg ctagacttta
     8401 gttccacaac actaaaccta taagttgggg aaatacaatg ttccagcaag aagttaccat
     8461 taccgctccg aacggtctgc acacccgccc tgctgcccag tttgtaaaag aagctaaggg
     8521 cttcacttct gaaattactg tgacttccaa cggcaaaagc gccagcgcga aaagcctgtt
     8581 taaactgcag actctgggcc tgactcaagg taccgttgtg actatctccg cagaaggcga
     8641 agacgagcag aaagcggttg aacatctggt taaactgatg gcggaactcg agtaatttcc
     8701 cgggttcttt taaaaatcag tcacaagtaa ggtagggtta tgatttcagg cattttagca
     8761 tccccgggta tcgctttcgg taaagctctg cttctgaaag aagacgaaat tgtcattgac
     8821 cggaaaaaaa tttctgccga ccaggttgat caggaagttg aacgttttct gagcggtcgt
     8881 gccaaggcat cagcccagct ggaaacgatc aaaacgaaag ctggtgaaac gttcggtgaa
     8941 gaaaaagaag ccatctttga agggcatatt atgctgctcg aagatgagga gctggagcag
     9001 gaaatcatag ccctgattaa agataaacac atgacagctg acgcagctgc tcatgaagtt
     9061 atcgaaggtc aggcttctgc cctggaagag ctggatgatg aatacctgaa agaacgtgcg
     9121 gctgacgtac gtgatatcgg taagcgcctg ctgcgcaaca tcctgggcct gaagattatc
     9181 gacctgagcg ccattcagga tgaagtcatt ctggttgccg ctgacctgac gccgtccgaa
     9241 accgcacagc tgaacctgaa gaaggtgctg ggtttcatca ccgacgcggg tggccgtact
     9301 tcccacacct ctatcatggc gcgttctctg gaactgcctg ctatcgtggg taccggtagc
     9361 gtcacctctc aggtgaaaaa tgacgactat ctgattctgg atgccgtaaa taatcaggtt
     9421 tacgtcaatc caaccaacga agttattgat aaaatgcgcg ctgttcagga gcaagtggct
     9481 tctgaaaaag cagagcttgc taaactgaaa gatctgccag ctattacgct ggacggtcac
     9541 caggtagaag tatgcgctaa cattggtacg gttcgtgacg ttgaaggtgc agagcgtaac
     9601 ggcgctgaag gcgttggtct gtatcgtact gagttcctgt tcatggaccg cgacgcgctg
     9661 cccactgaag aagaacagtt tgctgcttac aaagcagtgg ctgaagcgtg tggctcgcag
     9721 gcggttatcg ttcgtaccat ggacatcggc ggcgacaaag agctgccata catgaacttc
     9781 ccgaaagaag agaacccgtt cctcggctgg cgcgctatcc gtatcgcgat ggatcgtaaa
     9841 gagatcctgc gcgatcagct ccgcgctatc ctgcgtgcct cggctttcgg taaattgcgc
     9901 attatgttcc cgatgatcat ctctgttgaa gaagtgcgtg cactgcgcaa agagatcgaa
     9961 atctacaaac aggaactgcg cgacgaaggt aaagcgtttg acgagtcaat tgaaatcggc
    10021 gtaatggtgg aaacaccggc tgccgcaaca attgcacgtc atttagccaa agaagttgat
    10081 ttctttagta tcggcaccaa tgatttaacg cagtacactc tggcagttga ccgtggtaat
    10141 gatatgattt cacaccttta ccagccaatg tcaccgtccg tgctgaactt gatcaagcaa
    10201 gttattgatg cttctcatgc tgaaggcaaa tggactggca tgtgtggtga gcttgctggc
    10261 gatgaacgtg ctacacttct gttgctgggg atgggtctgg acgaattctc tatgagcgcc
    10321 atttctatcc cgcgcattaa gaagattatc cgtaacacga acttcgaaga tgcgaaggtg
    10381 ttagcagagc aggctcttgc tcaaccgaca acggacgagt taatgacgct ggttaacaag
    10441 ttcattgaag aaaaaacaat ctgctaatcc acgagatgcg gcccaattta ctgcttagga
    10501 gaagatcatg ggtttgttcg ataaactgaa atctctggtt tccgacgaca aaaaggatac
    10561 cggaactatt gagatcattg ctccgctctc tggcgagatc gtcaatatcg aagacgtgcc
    10621 ggatgtcgtt tttgcggaaa aaatcgttgg tgatggtatt gctatcaaac caacgggtaa
    10681 caaaatggtc gcgcctgtag acggtaccat tggtaaaatc tttgaaacca atcacgcgtt
    10741 ctctatcgaa tctgatagcg gcgttgaact gttcgtccac ttcggtatcg acaccgttga
    10801 actgaaaggc gaaggcttca agcgtattgc tgaagaaggt cagcgcgtga aagttggcga
    10861 tactgtcatt gaatttgatc tgccgctgct ggaagagaaa gccaagtcta ccctgactcc
    10921 ggttgttatc tccaacatgg acgaaatcaa agaactgatc aaactgtccg gtagcgtaac
    10981 cgtgggtgaa accccggtta tccgcatcaa gaagtaattc ttgccgcagt gaaaaatggc
    11041 gcccatcggc gccatttttt atgcttccgc cagcggcggc aaaatcaatt catcgctctc
    11101 atgctgctgg gtgtagcgca tcacttccag tacgcgcaac cccgcacggt gcaccgcatc
    11161 gtttaacgcc ttccctttca gcaaaccact gataagctga gcacaaaaca ggtcgccagt
    11221 ccctttcagg tcggttttta cccgtgaatg ggaaatgaca ttcacgctgt cggcactgac
    11281 caccaccacc tgcatctcct gattttcttc attaccggag gcgctggtaa tcaccaccca
    11341 ttttaatgtg tctgaaagca gactttttgc ggcagcaatg gcactgtcga gatcgcggca
    11401 atttttaccg gtcaggattt ccagctcaaa gatattgggg gtaattccct gcgccagcgg
    11461 cagtaaatat tgtcgatacg cctcgggaag gtcaggtttg acataaattc cgctatcaat
    11521 atcgccaatc accggatcga ccatgatcaa taggtcagga tggtctttgc gtagcgcagt
    11581 cagccactcg gcaaggattt tgatttgcga tgccgttccc atatagcccg tggttacagc
    11641 acgaagttgg cgcagcgcat cacgctcctg aagcgcacgc aaatagccgc taaaccattc
    11701 gtccggaatc gcaccaccgt agaaagtgtc ataatgcggc gtattgctca gcaataccgt
    11761 cggcacggca aagacattca agccgttctg ttttatagca ggcaccgcaa tgctgttgcc
    11821 tacgcttccg tacactacct gcgactgcac ggcgacgata tccgcctgca gcgccctact
    11881 cttatcgtta aacaacaaca aactactcat ttaatttttt ctccttgccg atgatcctca
    11941 tcgtattcca accgaaactt tacctgattc tggcagtcaa atcggctatt acaaaacaaa
    12001 gataaggtaa ttcagtgaag aaaatcattt gtctggccat tacgctgcta atgacactcc
    12061 ccgcttacgc gaagttaact gttcatgaag aagcccgcat caacgccatg ctggagggat
    12121 tagcacagaa aaaggatttg atatttgtgc gcaacggtga tgaacatacc tgcgatgaag
    12181 cggtttccca cctgcgtctg aagctcggca atacccgtaa ccgcattgac actgccgagc
    12241 agtttattga taaggtagct tcgtcgtcat cgattaccgg gaagccgtat atcgtgaaga
    12301 tccccggtaa gagcgatgag aacgcacagc cttttttaca tgcgttaatt gcgcagacgg
    12361 ataaaacggt gcctgcggaa ggaaattaat ccgctttggt aaggcattta caggaggtaa
    12421 catgaaaaaa tgctttattt atcacgatga aaaatcgaat aaattttggt ggatagatta
    12481 cgaaggggat agtttagctg tcaactatgg caaggtaggt agtattggta aattccatac
    12541 aaaagaattc gataatgaag aacagtgtct gaaagaagcc agtaaattga ttgccgcaaa
    12601 aatgaaaaaa ggatatcaag aaactccaga ctttaacttc atggatctct actattttga
    12661 tgatgaagag attgggttac atcttaaaac gtcacaccca aacttccagt gccattttac
    12721 tgatccactt tatatgtgtt gctgggatga agaatctcct tttggtagcg atgaaggtgc
    12781 tgatgctcta aacgaacttg aaaatagcct ccgttctgga ctgtgctgat ttccctcaaa
    12841 tgttaattga aactatgtgg ggtatgaaat acatcgctat ggacagtatt cttgaagagg
    12901 atgtgcgtgc gcaattacta gccgatgaaa tgagctctat ccagagcaat atgattacct
    12961 acgcgactgc attcggtcag atcaaagtca tgggtaaaat ctcccataaa cttaaaaaga
    13021 tgggactcaa tgcactagcg cgtcatcagc ttaccgcaaa aattcttcaa tggggtgacg
    13081 gtcaggactc accaatactt caaaaaatga ttgatgacct tactgctttt ccacacgaaa
    13141 attaaaaact gcatttgtcg gcagcaacaa ctgccgacaa atcagattat tgacaaagtg
    13201 cactttgttt atgccggatg cggcgtaaac gcctgatccg gcataaaaat attgctaatt
    13261 caatatattg cagaaacatt gtaggcctga taagcggagc gcatcaggca gttttgcgtt
    13321 tgtcatcagt ctccaatgct attaatcctt aaatccctgc cccctgacta aaatgctctt
    13381 ccccaaacac tccggtggag aggtagcgat cgccacgatc gcagatgatc gctaccacca
    13441 ccgcaccagg gttagctttt gccacccgca gtgctccagc aaccgcgccg cctgagctga
    13501 cgccacagaa tattccttcc cgcaccgcca gttcgcgcat ggtgttttcc gcatcgcgct
    13561 gatgaatatc cagcacctca tccaccagcg cagcgttgaa aatccccggc agatattccg
    13621 caggccagcg gcgaatgccc ggaatactgc tgccttcttc cggttgcagg ccaacaatgg
    13681 tcaccggttt ggattgttcg cgcataaagc atgaaacgcc ggtgatagtg ccggtcgtcc
    13741 ccatgctgga gacaaaatga gtgatgcgcc cgccggtttg ctgccagatt tccggcccgg
    13801 tggtggtgta atgcgcataa gggttatcgg gattattgaa ctgatcgagc agctttcctt
    13861 cgccacgatt cgccatctcc agcgccagat cgcgcgcacc ttccatgccc tgctctttgg
    13921 tgacaagaat cagttccgca ccataagcac gcatcgccgc acggcgttcc tggctcatgt
    13981 tgtcgggcat cagcaatttc atgcgatagc ctttcagcgc ggcaatcatt gccagcgcaa
    14041 tgccggtgtt accactggtg gcttcgatta agacatcacc cggtttaatt tccccgcgct
    14101 tttccgcctc gacgatcatc gaaagtgccg cacgatcttt caccgaacct gccgggttat
    14161 tgccttccag ttttaaccac acttcactgc cgttatccgg ccccattcgc tgcaacttca
    14221 ccagaggcgt attgcctatt gtttgttcta atgtactcac aatctctatc catacgtggt
    14281 gttgcctgat gcgacgcttg cgcgtcttat caggcctaca ggttacaaac cttgccatta
    14341 aaaagcccgg atcgcgggaa gcgcctccgg gcgtttaaca ttcactcaac ctatcaggcg
    14401 ctttgtgcga gagcaagttc ctcatcgcgg gtttcaatac gctcgtcgcc gttatacagc
    14461 cgcgcatgtt gcagaccaac gaataaacgc tcgccatgct gcggggcatc gtcgccatgc
    14521 atcacgaccg tcagcggttc gttgtaccac cccagcggct gcaccactaa ttgggtgtag
    14581 tgacctttcg ggctggcttc cagtacctgt accggcagcg gcgaatcgag actggtacgg
    14641 cggctgatat ccacttccca cgggcgcagg aagagatcca ccggcccctg atacgcaggt
    14701 gtgtagccca gcggccagcg atgcgcgcca acatggaact gcccgccgcg aatggttccc
    14761 tgcaggcggt tcacttcgcc cataaattcg agtacaaaac gggtcgctgg ttcgcgccat
    14821 acctgattcg gcgcatcagc ctgttcaata ttcccctgac tcatcaccac tacacgatcg
    14881 gctacttcgg tcgcctcttc ctggtcgtgg gtcacaaaca cgctggtgaa tttcagctct
    14941 tcatgcagtt gacgcagcca gcgacgcagc tctttacgca cctgcgcatc cagcgcgccg
    15001 aacggttcat caagcagcag aatttgcggt tctacagcca gcgcgcgcgc cagcgccaca
    15061 cgctgtttct ggccgccgga aagctgcgcc ggataacgat ccgccagatg cgcaagctgg
    15121 accatttcca gcaattttgt cactttcgct ttgatggctg cggcattcgg gcgctcgcga
    15181 cgcggcagca ccgtcaggcc aaaagtgata ttgtcgaata ccgtcatatg gcggaacagc
    15241 gcgtaatgct ggaacacgaa accgacttta cgatcgcgcg cgtgcaggcg actcacgtcg
    15301 gtgccgtgga agcgaatatg cccgctggtt tgatgctcca gcccggcgat aatccgcagc
    15361 agcgtggttt tcccggaacc ggacggcccc agcaacgcga ccatctgacc agaaggaata
    15421 tccagtgaga tatcgttcag cacctgggtg cgaccaaaag acttcttgat attggcaatc
    15481 tcaatgctca tgatgctcct cctgctgcgc gcgtttttcc tgattctcca ggcgccactg
    15541 caacatactc tttaaaaaca gggtgataat cgccatcaac gtcaacagcg cggcggcggt
    15601 aaaggagccg acggtgttgt agtcctgctc caacaattca atctgtaacg gcagcgaaag
    15661 tgtttcgccg cgaatcgagc cggaaaccac cgacactgca ccaaactcgc caatcgcgcg
    15721 ggcgttggtc agcaccacgc cgtaaagcag cgcccagcga atattcggca atgtgacgcg
    15781 acggaacatc tgccagccgg acgcgccaag caaaatcgcc gcttcgtctt cctggctgcc
    15841 ctggcttaac atcaccggca ccagctcgcg caccacaaac ggacacgtca caaagatggt
    15901 gaccagcacc attccgggcc aggagaacat aatttgcaga ttatgttcat ccagccagcc
    15961 gccgagcggg ccgttggagc cgtagaacag cagatacacc agaccggcaa ccaccggcga
    16021 tacagcaaac ggaatgtcca gtagcgtcag cagtaactgg cgtcccggga agttaaagcg
    16081 cgtcaccagc caggccagca gaatgccgaa caccaggttt accggtacgg caatcagcgc
    16141 gatcatcacc gtcagccaga tggcgtgcag catgtccgga tcggccagat tctgtaaaac
    16201 cggcatcagc cccttgctga atgcctgcac gaagatgtaa atcatcggca ccagcaggat
    16261 gaacgccgaa accagcatcc cgatgccaat cagaaaccat ttgccccagt taatcgggcg
    16321 cgcgtcataa cgcttcaatt gggtaacttc cgccattaat gacctaccac acgccgacca
    16381 aagcgacttt gcagagtgtt aattgagaac agcagcagca gagatgccgc gaggatcacc
    16441 gaagcaatcg cgctcgctgc cgggtaatca aactcctgta agcgcacaaa aatcatcagc
    16501 gacgtcactt ccgtcttcca cgcgatattt ccggcgataa aaatcaccgc gccaaattca
    16561 ccaagactac gggtaaacga cagcgccacg cccgccacca gcgccggaga aagctccggc
    16621 agcaccactt tgcagaaact ctgccagcgc gttgcaccaa gcgtttccgc cgcttcttca
    16681 tattccgggc ctaactcttc cagcaccggc tgcacggtac gcaccacaaa cggaatgctg
    16741 gtaaaggcca tcgccaccgc aataccaagc caggtatagg tgactttgat atcaaacttc
    16801 gccagccatt caccgtaaaa accgtttacg gaaaagagcg aggccagcgt taaaccggcg
    16861 actgccgttg gcagcgcaaa gggtaaatcc atcagcgcat caagcagcgt gcggcccgga
    16921 aagcgataac gtgttaaaat ccacgccatc agcagaccga aaacgccgtt aaaaatcgat
    16981 gccacaaacg ccgacagcag cgtcactttg taggccgcga ccacctgcgg gttggtgatc
    17041 acctcccagt actgcgccca gctcatctca gaaagctgca tcaccagcgc agagagcggc
    17101 agcagcaata tcaggcacac aaacagcaga ctggtgccaa ggcttaaagt aaagcccggc
    17161 agcacgcgtc ttgaagaaac tgcaaacatt acttacgccc cgccgctaac agcttgtcta
    17221 actcgccgcc gctggtgaag tgggttttca tcacttccgg ccatgagcca aatttgtctt
    17281 ccacgcggaa cagttcggtc tgcgggaatt tatctttcag tttgtccatg acttccgggt
    17341 tattcacacg gtagtaatag tcggtgatga tggtctgcgc ctgtgggctg tagagccagt
    17401 tcaggtaggc ttttgcggct ttttccgtac cgttggcctg cacattttta tcgacccacg
    17461 ccaccgggaa ttccgccaga atattggttt tcgggatcac cacttcaaag ccctgtgctt
    17521 catactgttt acggatgttg ttcacttccg attcaaagct aatcagcaca tcgcccaagc
    17581 cacgctcggc aaaagtggtg gttgcgccac gaccgccggt atcgaacact tcaacgtttt
    17641 tcaggaactg ggtcataaac tgttcggttt tggctttatc gccaccgtca gctttatccg
    17701 ctgcgcccca tgccgccaga taggtataac gcgcgttacc cgacgttttc gggttcggga
    17761 aaatcagctt cacgtcggag cgcaccaggt cgttccagtc gtggatattc ttcgggttgc
    17821 ccttacgcac caggaagccc atggtggagt agaacggcga gctattattc ggcaggcgcg
    17881 actgccagtc agccgggatc agcttgcctt tgtcgtgcag gatttgtacg tcggtcacct
    17941 ggttataagt gacaacgtct gcttttaagc cctgcaaaat cgccagcgcc tgttttgatg
    18001 acccggcatg agattgtttt atcgtcagtt tgtcgccgcc gttatctttt gcccattgct
    18061 gttcaaacgg cggattcagg gcggcaaaca gctcgcggga gacgtcataa gaactgttca
    18121 gcagttccgt tgcctgtaca tggcccgcca gcagcagaga agcgaccagc gcgagtgagt
    18181 tctttttcag taagttaacg gccattgcgc acccttataa atttaatgac tttctaatag
    18241 ccatcatatt tataacggac gtgaaaggag taacggtttt atataccgtt tggtgatttg
    18301 gaagttaaaa agggaataag aacttggttc aacaggcgta attgttgcag gcagtttgga
    18361 cacggacagc gcggagaaac cggagcgtac acatagtacg tgaggatttc gagcactgcc
    18421 cggggccaaa atgacaaatg aaatagcctg gtgaacttag ttcaagacga aatcctcccc
    18481 acacatgcag ggaggaaaca gaggtgaatc agataccgac gctaaccgtc tccggcagtg
    18541 tgctgccgcc atcaatcaca ttctgtgtac cggttaaata gctggattca tccgatgcga
    18601 ggaaggccgc cagttcgccg acttccagcg gatcggcgag gcgacacagc gggattgctt
    18661 ttgccatttc agtcagcacc gattctggat cttccgggtt cgactggcgg gcaatgcttt
    18721 ccgccattgg cgtgcggacg tatcccgggc agatggcgtt aacgcgaata ccggattgcg
    18781 cgtactccac cgccagcgat ttagtcaggc caacaatcgc cgctttcgtt aaggcatacg
    18841 ccgtttcgcc aggatcggcc accatatcac cagtgactga agacatcatc acaatgcgac
    18901 catctttgcg cgcaatcatc tccggcagca ccgccttcgt gacgttccat acgcctttaa
    18961 tattgatatc aatatggaaa tcgcgatctt catcgctcat atcgaggaaa ctgcccagac
    19021 gacaaacgcc tgcgttattc accaggatat caatgcgccc ttctttttcc ttcgcgcgtt
    19081 tgatagctgc ggctaccgac gccgggtcac gcacatcggc gacaaccgcc gtacagcgat
    19141 gaccacgacc acacagttcg tccgctagct tttcgatctc aggggagata tccagcaaga
    19201 ttaggttcgc gccatgacgt gcaaaagttc tggcaattcc ttcgccaatt ccctgcaatg
    19261 cgcccgtaat cagtgctgtc ttgcccgtga gtttacccat tttaatgctc tccttgttaa
    19321 tacagagcgt tacactccat tccctctgaa aatacaggat aaacaaaata ttgctgcgga
    19381 tcagctcact aattcaggaa aatctcaatc cagtcgctgc tttatgcatc atttcaggcg
    19441 ttgtgttagc tcactgctgc gctgaatcat tttcaatgac gccacatcat tgatgttgca
    19501 caagaccaac aaacagtaaa tcggtaacag aattttgcgc cgtgcgggta gacatcgacg
    19561 agctacgcca ttcggtttca ccggaaacgg tatcgagcgt gaaatgcgcc agccgccgca
    19621 gcggagagtc agccagagaa gtaatggcaa tcaccgttgc cccctgcttt cgtgccgctt
    19681 ctgcgcacaa gacaatctct tttttactgc cgctgtaaga gatagcgatt tgtacatcgc
    19741 cttttttcaa cgcctgggaa acggtggctt gtacgtgcgt gtcggcttcg caggccacgc
    19801 gatagccaat tttcatcagt ttgaacgaca gatcgcgccc caccagcgcc gatccgccca
    19861 ggcccgttat ctggataaac ggtgctttac tgatgacctc aatgattttt tgcagccgcg
    19921 cgtagtcgaa caacgcacag gtttgctcca gcgccagctc tttttcgcga ttcagtttgc
    19981 gggcaatcac ttccagcgaa tcatcactgg taatcgaact gtgcaggtgc agcgccgtgg
    20041 cattcgtttt ctcccggctg gcactgtatt cgccaattaa cgccatacgc aattcagtaa
    20101 atccttgcgc gccgagcttc tgggcaaatt tcacaatgct cgactggctg ataccgagct
    20161 gtttcgccat ctgccgtgaa gagacggatt tcagctcgct cactctggcc cgcagaaagt
    20221 cagcaatttt ttgctcgttc ccggtaaatt cactttccgc attgcgtatt ttcgtcaggt
    20281 acaacatctt ccactctcct gggctggcag caaaaattcc gcttaagttc ttatgacgct
    20341 cttcacactc tgcgagtgta gactcaatga ttcctttaag tctgtcaatt cgggatatat
    20401 gattccatac atctgcaatt atttgatcgt aaatagtaag gtcaccaccg atgcaactag
    20461 aaaagatgat tactgaaggc tcgaacaccg cctcggctga aattgaccgc gtatcgacgc
    20521 tggaaatgtg ccggattatc aacgatgaag ataaaaccgt accgcctgcc gttgatcgcg
    20581 tcctgccgga tatcgccgcg gcgatcgatg ttatccacgc ccaggtcagc ggcggcggtc
    20641 gtctgattta tctcggtgcg ggaacatccg gtcgtctggg gattctggat gccagcgaat
    20701 gtccgcccac ctacggcgtg aaaccgggtc tggtggttgg tttgattgct ggaggcgaat
    20761 atgccattca gcacgcggtg gaaggagctg aagatagccg ggaaggtggt gttaacgatc
    20821 tgaaaaatat taatttaacg gcacaggatg tggtggttgg cattgctgcc agcggtcgca
    20881 cgccgtatgt gattgccgga ctggaatacg cacgccagct cggctgccgc acagtgggaa
    20941 tttcctgtaa tccggggagc gccgtttcaa ccaccgctga gtttgccatt acaccgattg
    21001 taggtgccga agttgttacc ggttcttcgc ggatgaaagc aggtacagcg cagaaactgg
    21061 tgctcaatat gctttccacc gggctgatga ttaaatccgg caaagtgttc ggcaacctga
    21121 tggtcgatgt ggtcgccacc aacgaaaaac tgcatgtgcg ccaggtcaat atcgttaaaa
    21181 acgccaccgg atgtaatgcc gaacaagcgg aagcggcgtt aattgcctgc gagcgcaact
    21241 gtaaaacggc cattgtgatg gtgctgaaaa atctcgatgc cgcagaagct aaaaaacgcc
    21301 tggatcaaca cggcggcttt attcgtcagg ttttagacaa ggaataaccc atggccaaag
    21361 agatcagcag tgaacttctg aacaccattc ttacccgtgt cggcggaccg ggaaatatcg
    21421 ccagttgtgg taactgtatg acgcgcctgc gtctgggtgt acatgacagt tcactggttg
    21481 atcccaatat caaaacgctg gaaggcgtga agggcgtcat tttgaccagc gaccaggtgc
    21541 aggtcgtttt tgggcctggt aaagcgcatc gtgccgcaaa agcgatgagc gagctgctgg
    21601 gcgatgcacc ggtacaggat gccgcagaaa tcgccgccca gaacaaacgt cagttaaaag
    21661 ccaaacaaac ctccggcgtg caacaatttc tcgccaaatt cgccaccatc ttcacgccgc
    21721 tgatccccgg ttttattgcc gccggtctgc tgctggggat agcgacgtta attgccacgg
    21781 tgatgcacgt tccggcagat gctcagggca cgctacccga cgcgctgaat tttatgaagg
    21841 tgttcagcaa aggtttgttc accttcctag tgattctggt gggctataac gccgcccagg
    21901 catttggcgg cacgggcgtc aatggcgcaa ttattgccgc actctttttg ctcggttata
    21961 accccgctgc aaccaccggt tactacgccg gttttcacga tttctttggt ctgcccatcg
    22021 atccgcgcgg caatattatc ggcgtgctga ttgccgcgtg ggcctgcgcc cgcattgaag
    22081 gcatggtgcg ccgctttatg ccggacgatc tcgacatgct gctgacctcg ttaatcaccc
    22141 tgctgatcac cgctacgctt gcgtacctga tcattatgcc gctgggtggc tggttattcg
    22201 aaggtatgtc atggctgttt atgcacctga acagtaatcc gttcggttgt gcggttttag
    22261 ccgggctgtt cctgatcgcc gtggtatttg gcgtgcatca gggctttatt cctgtttacc
    22321 tcgcgttaat ggacagccag ggattcaaca gcttatttcc catcctttca atggcaggcg
    22381 cgggccaggt gggcgcggcg ctggcactct actggcgggc gcaaccgcac agtgcgctac
    22441 gcagtcaggt acgcggggcg attattcccg gcctgctggg cgttggtgaa ccgctgattt
    22501 acggtgtcac cctgccccgc atgaagccgt ttgttaccgc ctgtttaggc ggcgcggcgg
    22561 gcggtttgtt tatcggattg atagcctggt ggggtctgcc gatgggctta aacagcgcct
    22621 ttgggccgtc tggtctggtg gcgctgccgc tgatgacttc cgcacaaggc atccttccgg
    22681 caatggcggt ttatgctggc gggattctgg tggcatgggt ttgcgggttt attttcacca
    22741 cgctctttgg ctgccgtaac gtcaatctgg actgatatta tgaaacggac aatgctctat
    22801 ctttctctgc tggctgttag ttgtagcgtc agcgccgcaa aataccctgt tctgacagaa
    22861 ggtagttgat caagtatatg cggcgttaaa gcagtaataa aaaggccagt cagtaatgtt
    22921 actgcctggc ctttaatcaa tgaatcagaa acgcttacag cgccatcaac ttgtccagcg
    22981 acggtgcgaa ataatagccg ccggttaccg gtttggtgaa acgcaacatc gcatcacgtt
    23041 tgccatcggt atcgccaaac atgctcagca gttgctgctc aatgttatgc agacgcgcgc
    23101 agtaggcgca gaagtacaga ccgtgagtgc cactggcggt gccgtacggc aggctctggc
    23161 gaacaatctt cagccctttg ccatcttctt tcagatcaac gcgggtgagg tgagaggttt
    23221 ccggacgttc gtcgccgtca atctcttcgt tggcctcttt ggtgcgcccg atcatcatct
    23281 cctgatcgtg aacgctcatc cggttgagct gcttcaggtt gtgctcccaa cgctgcacaa
    23341 acacatagct gccgccggaa tccacgccgt ctttgataac cgccacttcg cggcgcgtct
    23401 cttcacccgc cgggttttct gtaccgtcaa caaagccgct caggtcacgc tcttccaccc
    23461 aacggaagcc gtggatctct tctttcactt caatgcagtc accaaaggct tccatcgccg
    23521 cctgagcgac agagaagttt acgtcgtgac gcagagaaag aatgtggatc aacacatcga
    23581 actgggtcgt cggcgcaagg cctttaccgt agcccggaaa atctttcagc tcttctgccc
    23641 caacgccgcc gctcagagcg cgccaggtgt tgttaccaaa ggcaaccacc gcaccaagat
    23701 gcgcgtccgg gaatttcgct tcaaaagttg ccagtttgtc ggcaaatgtt ttactggccg
    23761 cacgcagggc gtcaacttcc cctttcacgt tggcttcgat ccaaatcgcc gcgcggcaat
    23821 gttctggcaa aatgccactc tgaacctgag acattgttcc tcctgaaaat aagaatgcca
    23881 cgcaatccgt ggcattgttg agcgctattt tacgtgtttt ttcgcgacgg cggtttgttc
    23941 agacgcaaat taacgccgcc agataatttt actgactttc cagtttttca gggtatcgtc
    24001 cgaaggcatt aacccttcag gaccacgcca ttccccgctg aactggtagc taatatgttg
    24061 actgccttcg gctttacatt cgacggcacg attatcatca ccatcagctt tttggcaatt
    24121 gccaaatgct ttgctgtaaa ggtcgctaaa cggtgtaccg attttaacac cggtgtcagc
    24181 aggaatatcg ctatccagca cgtcaatgcg actgatcgtg ccttgatcgc cgttaatcac
    24241 catcgccacg ttgtcgcctt tcatcacttc aaaaaagcgc accacgttgc cgttcgtggt
    24301 tttcattccg ctgcgcaggc gataatcgcc atcaagcgca tcagcaatgg cttgttcttg
    24361 cagtggtgtg gacgccgtta attcacccac gccctgctcg ctcactttgg tggacgatcc
    24421 aaaccagttc cacgggttag cggcagacca gttaactgac gacatcgtgg aacagccggt
    24481 cagcatcaac ggcatagcgc ataacattaa acgcagcgat ttcatgtcac ttcctttggt
    24541 tattcaataa cgttgcttgg agtgcaaatt caccaaaaag tgccgttatt ctttttcttc
    24601 attaaaacaa gcgcgtaaac gtcgattggt cagcagccag attaacgcca caatatccgc
    24661 cactaccagc gccagaccga taccgctaac ggattcaccg ttcagccaca gccacggttg
    24721 ccagcaaagt aaaaccacct gcgccaacag caacagaaaa tagagcacat gccaggtgcg
    24781 aggaaacgaa gcccgccgac cgctcaacaa aaacgccagc accgccggaa taccgggaat
    24841 cagccccagc cagaaattat cgtgatcggg ataaaacaga tttagcagcg cagtaccctg
    24901 ctcacgcgac gcaccggcaa tgacaaacag cacccaggtt cgcgcctgaa gcaatagcac
    24961 aagccagaag agcaagggta aacgcaggcg tccgtgcgca tcataatgga caggatggaa
    25021 ctcagtactc ttcatcttca atcaaacgct tacccagact cagcacgtcg gcgtgttcat
    25081 atcccaggcg ttcatacatt ccgagcacca tgtcgttatc ttccggcacg ttgatctgaa
    25141 ttttcgggca gccgcgagca atcagctttt tctccagccg gttaagcaac gcattggcaa
    25201 ttccacgccc acgaaactct ggatgcacgc caagataata agcagacccg cgatgcccgt
    25261 cataaccgcc catcaccgtt ccgaccactt caccgtttac ctcagcgacc aaaaacaaac
    25321 tgacgtcatg gttcatctta cgctcgatgt ccatttccgg atcgttccac ggacgcagca
    25381 agtcgcaacg ctcccaaagg gtgatgacct cttcgaaatc ttcctggcga aatacgcgta
    25441 tctccatggt attcgttacc tttttgcggg ttaaaaggct gattatggcg tgaacggtcg
    25501 aattagccaa tatctgacga aaatcggttg aaaaagtggc ataatgggga gttgtcaact
    25561 attgaaatga aaagtaaaac aattctcaac agcaaaccgt cgtaacggat tacgcgatac
    25621 gatataacat ctggaacttt attattacaa ctcaggccgt atgagcactt ttaaaccact
    25681 aaaaacactc acttcgcgcc gccaggtgct gaaagccggt ttagctgccc tgacgttgtc
    25741 aggaatgtcg caagccattg ccaaagacga acctttaaaa accagcaacg gacacagcaa
    25801 gccgaaagcc aaaaaatctg gcggcaaacg tgtcgttgtt ctcgatccag gccacggcgg
    25861 aattgatacc ggagcgatcg gacgcaacgg ttcgaaagaa aaacatgtgg tgctggcgat
    25921 tgctaaaaac gtccgttcca ttttgcgtaa tcatgggatt gatgcgcgtt taacgcgttc
    25981 tggcgatacg tttatcccac tttacgatcg cgttgaaatc gcccataaac atggcgcaga
    26041 tctgtttatg tcaattcatg ccgatggctt taccaacccg aaagctgccg gtgcttcggt
    26101 atttgccctc tctaaccgtg gggcaagtag cgcaatggcg aaatacctgt ctgaacgcga
    26161 aaaccgcgcc gatgaagttg ccggtaaaaa ggcgactgac aaggatcacc tattgcagca
    26221 agtactgttt gatctggtgc aaaccgatac tattaaaaac agcctgacgc ttggctcgca
    26281 tattctgaaa aagattaagc cggtgcataa actgcacagc cgcaacaccg aacaagccgc
    26341 gtttgtggtg ttgaaatcgc cgtcggttcc ttcggtgctg gtggaaacct cgtttatcac
    26401 caacccggaa gaagagcggc tgttaggcac ggcggcgttt cgtcagaaaa tcgccacagc
    26461 gattgctgaa ggcgtgatca gttatttcca ctggttcgac aaccagaaag cacattcgaa
    26521 aaagcgataa gttatgaaac ccgacgcaca ccaggttaaa cagtttctgc tcaaccttca
    26581 ggatacgatt tgtcagcagc tgaccgccgt cgatggcgca gaatttgtcg aagatagttg
    26641 gcagcgcgaa gc
//
LOCUS       DAJTSI010000050        25812 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000050 DAJTSI010000000
VERSION     DAJTSI010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25812
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.050"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..342)
                     /locus_tag="OO327_001823"
     CDS             complement(<1..342)
                     /locus_tag="OO327_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018387.1"
                     /translation="MLGVIQNLCCCRPDSPLADSKQRNQKLPQQDFEDFKNDVINVIN
                     NNEDIYFLRESNLLNAFIRTCKKRDDNSYGNMIFTLYFDNDIYGNRIIKASLNGGELQ
                     LLSTGCSNDNIL"
     gene            692..1714
                     /locus_tag="OO327_001824"
     CDS             692..1714
                     /locus_tag="OO327_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HCR6018388.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            1714..2694
                     /locus_tag="OO327_001825"
     CDS             1714..2694
                     /locus_tag="OO327_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR6018389.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            2691..3449
                     /locus_tag="OO327_001826"
     CDS             2691..3449
                     /locus_tag="OO327_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6018390.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHTLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            3459..4268
                     /locus_tag="OO327_001827"
     CDS             3459..4268
                     /locus_tag="OO327_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR6018391.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            4268..5122
                     /locus_tag="OO327_001828"
     CDS             4268..5122
                     /locus_tag="OO327_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HCR6018392.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            5148..5705
                     /locus_tag="OO327_001829"
     CDS             5148..5705
                     /locus_tag="OO327_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HCR6018393.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            5642..7102
                     /locus_tag="OO327_001830"
     CDS             5642..7102
                     /locus_tag="OO327_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR6018394.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(7152..7406)
                     /locus_tag="OO327_001831"
     CDS             complement(7152..7406)
                     /locus_tag="OO327_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR6018395.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            7607..8341
                     /locus_tag="OO327_001832"
     CDS             7607..8341
                     /locus_tag="OO327_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR6018396.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(8343..8954)
                     /gene="emtA"
                     /locus_tag="OO327_001833"
     CDS             complement(8343..8954)
                     /gene="emtA"
                     /locus_tag="OO327_001833"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR6018397.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            9054..9968
                     /gene="ldcA"
                     /locus_tag="OO327_001834"
     CDS             9054..9968
                     /gene="ldcA"
                     /locus_tag="OO327_001834"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR6018398.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            10063..11799
                     /locus_tag="OO327_001835"
     CDS             10063..11799
                     /locus_tag="OO327_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR6018399.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            12410..13824
                     /locus_tag="OO327_001836"
                     /pseudo
     CDS             12410..13824
                     /locus_tag="OO327_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13992..15062)
                     /gene="dadX"
                     /locus_tag="OO327_001837"
     CDS             complement(13992..15062)
                     /gene="dadX"
                     /locus_tag="OO327_001837"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCR6018400.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(15072..16370)
                     /gene="dadA"
                     /locus_tag="OO327_001838"
     CDS             complement(15072..16370)
                     /gene="dadA"
                     /locus_tag="OO327_001838"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR6018401.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            16700..18232
                     /locus_tag="OO327_001839"
     CDS             16700..18232
                     /locus_tag="OO327_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR6018402.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            complement(18284..19003)
                     /gene="fadR"
                     /locus_tag="OO327_001840"
     CDS             complement(18284..19003)
                     /gene="fadR"
                     /locus_tag="OO327_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR6018403.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            19224..20765
                     /gene="nhaB"
                     /locus_tag="OO327_001841"
     CDS             19224..20765
                     /gene="nhaB"
                     /locus_tag="OO327_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR6018404.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            20911..21441
                     /gene="dsbB"
                     /locus_tag="OO327_001842"
     CDS             20911..21441
                     /gene="dsbB"
                     /locus_tag="OO327_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR6018405.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(21487..22755)
                     /gene="umuC"
                     /locus_tag="OO327_001843"
     CDS             complement(21487..22755)
                     /gene="umuC"
                     /locus_tag="OO327_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR6018406.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(22755..23174)
                     /locus_tag="OO327_001844"
     CDS             complement(22755..23174)
                     /locus_tag="OO327_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR6018407.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            23547..24447
                     /gene="hlyE"
                     /locus_tag="OO327_001845"
                     /pseudo
     CDS             23547..24447
                     /gene="hlyE"
                     /locus_tag="OO327_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            complement(24654..25099)
                     /locus_tag="OO327_001846"
                     /pseudo
     CDS             complement(24654..25099)
                     /locus_tag="OO327_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
     gene            complement(25191..>25812)
                     /locus_tag="OO327_001847"
     CDS             complement(25191..>25812)
                     /locus_tag="OO327_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR6018408.1"
                     /translation="YPVSKVVCVGSNYAKHIKEMGSAVPEEPVLFIKPETALCDLRQP
                     LAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDLTLRDVQGKMKKAG
                     QPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQGTTADMIHKIVPLI
                     AYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRVL"
BASE COUNT         6360 a         6667 c         6316 g         6469 t
ORIGIN      
        1 gagaatgtta tcattgctac accctgtgct taataactgc aactctccgc cgttcagcga
       61 tgctttgata attctgttcc catagatatc gttatcaaaa taaagcgtaa atatcatatt
      121 tccgtaactg ttatcatctc tttttttgca cgtccgaata aacgcattca gtaaattact
      181 ctctctaaga aaataaatat cttcattgtt gttaatgaca ttaataacgt cgttcttaaa
      241 atcctcaaag tcttgttgcg gtaacttctg gttacgctgc ttactatcag caagcggtga
      301 atccggacga caacaacata ggttttgtat aacccccagc attcttttca cctccgttaa
      361 cagtatattc acgacactga aacgagagaa tgctgataat tttctcggcg agatatcaaa
      421 aatcgctcat tataaaacaa gtaacttcaa cgtcgcgtaa ccgtggacga taaaaaaaca
      481 ataatgtcat tcatatttaa ataatttagc cttaaatgat ttaaataata ttagccacct
      541 gataaataca tttttaatga tagagagggt caacaggctt gcggaagtcg tcacatcttc
      601 atgttttcaa tagctaaact ttgatattga acatgatttt tatcgatata tatgaaagta
      661 tatatcgatt ttacttttca ggatgttcac catgcccata accagacgga cgtttgcgca
      721 ggcacttgca tctaccctgc tcctgcagtc tctaccgtcc ttttcacaaa cagttaatcg
      781 ttttgcttcg caatctcttc ccgaagccca aaacattaca cgcatcgtca gcgcaggcgc
      841 gcccgccgat ttgctcttgc tggctgtcgc gccagaaaaa atggtcggtt tttcctcttt
      901 tgattttgcc cgtcaggcat taattccatt gccagagcac attcgccagt tacccaggct
      961 gggacgactc gccgggcgcg ccagcacact atcgctggaa gggcttatgg cgttacatcc
     1021 cgatttggtc gttgattgcg gcaatacgga tgaaaccttg atctcccagg cacggcaggt
     1081 tagcgaacag acacaaatcc cctggttatt gcttaacgga aaactggcgc aatcagcaga
     1141 acagttaaca acgcttggca aaacgttagg cgaagagcac cgcgccgcag aacaagccaa
     1201 tctcgccagc cacttcgttg gtgaagctca ggcattcgcc acctcacccg ccgctaacct
     1261 ccgcttttat gctgcgcgcg gtccccgagg gctggaaacg ggcttacagg gttcgttgca
     1321 taccgaggcg gcggaattat taggtttgca caacgtcgcg caaatagccg atcgccacgg
     1381 cctgacacag gtttccatgg aaaatctcct gcgctggcag ccggatatta ttctggttca
     1441 ggaggccgtt actgcagatt ttattcgtcg tgatcctctc tggcagggcg tgaaagccgt
     1501 tgcggaacaa cgcatcttat ttttaagtgg cttgcccttt ggctggctcg atgccccgcc
     1561 gggaatcaac cgtcttctgg gattacgcag acttcacgcc tggctggatc ccgccatcaa
     1621 tcgccagttt aaaagtgaca tgcagcatta cgcccaactg ttctggcatt gttcactcag
     1681 tgatgccgac tatcaaaaat tggtggcgag ctaatgagaa tcgtaaacgg atgtattttg
     1741 ctgctggcag ctatcagtat cacttttgcc gccgtaagtg gggcttatca tctcgatatg
     1801 cagcaactgc tggcgctaat tcttcgtcaa gaaaatgagt ctgtgcagga acaaattgtc
     1861 ttctggcaaa tccgcgtgcc gcgtattctt gctgcgctgt tactgggcgc agcgctggca
     1921 ggagccggaa ccacctatca gggaatgttg cgtaatccct tagtttcacc ggatatttta
     1981 ggcgtctccg ccggtgccgg acttggtgcc tgcgcggcaa tcctgtgggg attacccatt
     2041 gtttacatcc agctgtacgc attttgcggc gggctgatgg tggtcgcggg cgtctggttg
     2101 attacgcgtc gggtgacccg ccacgatccg atcttaaccc tggtgctggt aggcattgcg
     2161 ttaggaacgt tgtgcggagc ggggatatcg ctaattaaaa cgctggccga tccttacacc
     2221 cagttaccgt caattacctt ctggttgctg gggggactgt ccaccgtgac cttacgcgat
     2281 ctctgctatg ctgcaccgat cattctcata ggctccttgc ccctgttttt tctccgctgg
     2341 cgcatgaatt tactcacgct ttccgacgac gaagcccgtt cgctgggact gaacgtgacg
     2401 cgtctgcgct ttggattgat tgtctgcgcc acactaatca ccgccagcac ggtagcaata
     2461 gcgggcataa ttggttggat cggtctggtc gttccgcaca ttgcccgctt gttgaccggt
     2521 cataaccatc agcagctgtt gcctatggca atgtgtactg gcgctattct gctcctgctt
     2581 accgatacgc tggcgcgcag cattggaaca acagaaatcc cacttggcat tctgaccgct
     2641 tttgttggcg caccgttttt cctgcttttg ttgttacgcg gaggtcgcca gtgactttgc
     2701 ttgccgttcg tcatgcgtct ctgggttact cccgccatcc agtgctacgc gacgtgtcct
     2761 tcaccctgtc gcaaggaaca atttgttgtc tgcttggcgc gaatggttgc ggcaaaacca
     2821 cactcatgcg ctccattctt ggcgttatac cgttattaaa aggcgagatc ctactggata
     2881 gcctctctgt gcagacactc agccatcgcc agcgcgcaca ggctattgcc tgggtgccgc
     2941 aggcgcacga cggtattttt gctttcagtg tgctggatat ggtgttgatg ggcctggccc
     3001 ccaccatagg cgctttttcc gttcccggta agcaagaacg cctgaaagcc acggagcaac
     3061 tggaaaagct gggcattttg catctggcga cacgacgctg gaatacgctt agcggcggtg
     3121 aacgtcagct ggctttaatc gcccgtgcac tcgtccaaca accgcgcttg ttgttactgg
     3181 atgagcccgc ctccagcctc gatttcggtc atcagatcca acttctggac acgctggcgc
     3241 agctcaaaaa taacggcatg acaatgttaa tgtcgactca ccatacgctc catgccaacg
     3301 ccattgccga cagcattatc caagtagagc cagatggacg agtaacacaa ggcttaccga
     3361 ccgaacagtt aacaacgaac aaactggcgg ccctgtatcg ggtttctgcc gaccaaattc
     3421 atcatcacct ctctgcaatt agccgctaag gaacaatcat gctcattgat gatattgatt
     3481 ttgcagacct ttatctgcag caactcaaac tggctcaccg cacagaaaaa acgcccgatc
     3541 actgggatca acgggcggag aaaatggcgg aaaattgcgc ctcgccaacg gacagctacc
     3601 tgcaacaact gatcgctaaa attgacctgc aaggcgctca gaccttattc gatatgggct
     3661 gcggaccagg caccgtatcg ctggcgctgg ctgacaaact cactacggtt tatggcgttg
     3721 attacagtca ggggatgctg aacgttgccg cccgccgcgc cgccgcactg aaggctgaca
     3781 atgtccactg gatccagcgc gcctgggaag aggactggag tgacctgcca cgctgcgata
     3841 ttgccgtggc ttcccgctcc actctggtgg ctgatatgcg ccatgcaatg agcaaactga
     3901 ataatcaggc acggcttcgc gtctatacaa ctcatctggt cagcacctcg ttcgtctctc
     3961 cagccattca gcgggcggct ggtcgggaag tgatcgaatt acccaactat atctttgcgc
     4021 tcaacgtcct gtatcagatg ggaatttatg cccacgttga ttttatccgt ggacaaaatt
     4081 gccaacagga taacagcacc tgggaacgtt ttgagcagaa cgtcagctgg agtcttggcg
     4141 cgcttaacga cgatgagcgt gagcggttat atcactggta tcaacaacaa gatgctcgtg
     4201 ccctcgcccc tgccagccgc gactgggcgc tcatctggtg ggatagcgta ccgcaggagg
     4261 cactgtgatg attttccttt ctcaggcaca aatcgatgcg ttactgctgg aagatatcca
     4321 gggaggtgac ctgaccacgc gagcattaaa tattggacac cagcctggct atatagcgtt
     4381 ttttcaccgt cagggcggct gcgtcagcgg cgtttctgtc gcgtgtaaga tgttaactac
     4441 gctgggatta actattgatg acgcggtcag cgacggttca caagcgaacg cgggtcaacg
     4501 gctaatccgt gcgcagggta atgccgcagc acttcatcag ggatggaagg cagtccagaa
     4561 tgtgctggag tggagttgcg gtgtttctga ttatctcgct caaatgctgg cattgcttcg
     4621 tgaacgttat cctgacggca atatcgcctg cacccgaaaa gcaattccgg gcacccgctt
     4681 actggcttcg caggcaattc tggctgccgg aggactaatt catcgtgccg gatgtgcgga
     4741 aaccatatta ttgtttgcca accaccgcca ttttcttcat gacaatcagg gctggtcagg
     4801 cgcaatcgct cagttacgtc gccacgcacc cgagaagaaa attgttgttg aagccgacac
     4861 gccgaaagag gctatcgccg cgttacgcgc acaaccagac gtacttcagc tcgacaaatt
     4921 tagtccgcaa caggcaactg aaattgcgca aatagcgccg tcgctggctc ctcactgcac
     4981 gctggctctg accggcggaa ttaatctgac gacactcaaa aattacctcg actgcggcat
     5041 tcgccttttt attacctccg cgccttatta cgcggcacca gctgacatta aagtcagtct
     5101 gcaacccaca gcctctattt aattctttgt taaacggaat ttattctatg agactcaaaa
     5161 aacgttatct ctgcacagca ttatcacttg cctttaccca gcaggccgta gcggctcagg
     5221 agagcgacac gctgaccgta tggtccagtc cggtatcatc gacgacgacc accgttctcg
     5281 atcaacccac catgaaggcc ttggataaac agaatgtcgc tcaggcatta agtgtcgtcc
     5341 ccggcgtggt gctgcaaaag tcaggcagcc gcaacgaaga acaggtaaaa gttcgtggct
     5401 ttgatagtcg gcaggtgccg gtctatttcg acggtgtgcc catttatgtc ccctatgacg
     5461 gcaacctcga tctggcgcgg attctgacca acaatctggg ggcagtcgaa gtttccaaag
     5521 ggtattcgtc gctgcttcat gggcctaatc agatgggcgg agccattaat atcaccaccc
     5581 tgaagaagca agtctgggat atcgccaggg atggagccgt agtcaggaca atgcctacga
     5641 tatgcatgct tcatttgccg ccagcagcga tcttgggtat ttgcaagtca gcggtagcca
     5701 gctaaagcag gattttctcg gcctgccgca tggtgtaaat aatgatattg caggcaaaca
     5761 cggcaagatg attaattcat cggctgatga taaacgcggc attgtgaagc tcggttttac
     5821 accgcgcgaa aacgatgaat acacgctgac ttacattaag caggatggtg aaaaagataa
     5881 cccgccatac agcggaaatt gtggtcaaaa atcacgctac tggcagtggc cagagtatga
     5941 caaagaaagt ttttattatc agggagcgac ccaactaaac gaccgtttta ccctgaaaag
     6001 tcggctgtat cgcgacacct ttgaaaatac gctgatgatg tacaactcgc tggctgattt
     6061 gaaaaataaa aaaggcagct acagccatta ttccgattac agcgacggtg ccggattaca
     6121 actggcagcc gatgtgcgtg aaaacgatct gctgtcgttt gccgttaact ggaaagatga
     6181 cgtacaccgg gaaaaaggtg cgccgcacgc cgcttacgat cgctatgaag atcgtacctg
     6241 gtcgctcgcc agtgaatatc aatgggctgc tgctgataat gtcgatgtcg tggcgggaat
     6301 cagctatgac tggcgcgata gcgtagaagc gaaaaaacac gagaaagatg gcagcatcac
     6361 ccattatgac gacaacaatc agtcagcttt taactggcag gtgatgggga aataccactt
     6421 tgccaatgaa gacactctgg cgctttcgta ctatgaccgc acacgctttc cgacgctgaa
     6481 agaacgctat accacatcca aacctgcgta taaccagata gcgattgtta acccgcagct
     6541 caagccggaa cgcgctcgcg gggtggattt aacctggaat ggtgccttca cgcgcgactg
     6601 gggatttgag gtcagcgttt actataaccg ggtgagtgat gccatcctct cgcacaatat
     6661 caatgccgat accattcaaa atcagaacag cggcacggtg gattacagcg gtctggatgc
     6721 cggtattaag gggaaaatca gcaatatact ggatgtagga ttgagctacg ctctgatcca
     6781 cgctgacgcc aaacgcaaag acatcggcaa gataaccgat cttccaacgc agacaatgac
     6841 cgcatggatg acactcaaac catgggagcc gttaagcgta acgctgtcgg aagaggcgcg
     6901 ttcctccagc tacagcaaca gtgacggttc acaaaaagcc gccggttttg cggtgaccca
     6961 cattcgagcc gattacacct taggtcatgg cttcagcgtc aatgcgtcgg tcaataacct
     7021 gtttgatacc caatatgcct acagtgaagg gtttattgaa gaaggtcgca atttctgggc
     7081 aggtgtcgag tatacgttct gatgtgaaag cggatagcgg catctgtatc gctatccgcc
     7141 ttatgaaaat cttattctct tcgcaggagg cggaatacgc ccagcaccag gatagcgccc
     7201 accaccgcca ccaggaagct gtgcaaatta aaaccgctga tggagccgtc aatgccaaac
     7261 atggtcgcca gccagccgcc gaccaccgca ccgactatcc cgagaataca ggtcaggaaa
     7321 aatccaccac catcacgccc cggcatgatt agcttgccga taatgccggc tatcaggcca
     7381 aaaataatcc aggcaataat tcccatgttc aggccctcgt ttcgatgaat cggtcgcaag
     7441 aaatgcttac gtcaacgctc taagtatagg caagggcggg atcgatgctg ggcgtttgtc
     7501 ggctgattta agaacaacat gtaaaaaata ttcacgcatt tgtattaagt tttgttaact
     7561 atgaccgata aaccaaagac agtttgtcag tcaggagttt ttccgcgtga gtcattacca
     7621 tgagcagttc ctgaaacaaa atccgttagc cgtcctgggc gtgttacgcg atttgcacaa
     7681 agccgcaatt cctttgcgtc tcagttggaa tggcgggcag ctgatcagca aaatactggc
     7741 aataaccccg gataaactgg tgctggattt cggcagtcaa gccgaagaca acatcgccgt
     7801 gctaaaggca cagcacatta ccattaccgc cgaaactcag ggtgcgaaag tcgagtttac
     7861 tgttgaacaa ctacagcaga gtgaatactt gcagcttccg gcatttatta ccgtaccgcc
     7921 tcccacctta tggtttgtac aacgacgccg atatttccgc atctccgccc cactccatcc
     7981 gccttatttt tgccagacca aactggcgga taacagtacg ttacgtttcc gcttgtatga
     8041 tttgtcgtta ggcggcatgg gcgcattact ggaaacagca aagcctgccg aattacaaga
     8101 aggtatgcgc ttcgctcaaa ttgaagtcaa catggggcaa tggggtgttt ttcactttga
     8161 cgcccagtta atctccacca gcgagcgcaa agtgattgat ggcaagaatg aaaccatcac
     8221 cactccccgt ctgagcttcc gttttcttaa cgtcagcccg acggtggagc ggcaattaca
     8281 gcggattatt ttctctctcg agcgagaagc ccgggaaaaa gcggacaaag tgcgcgactg
     8341 atttacatcg cgtccagtgc ctgctcaagt ttgtagatat agcgcggagc ctgcggcgca
     8401 ggatgatttc gcgctacgtg atcgaggaac tcgtcagcat ccaaatcgtt gattttgctg
     8461 atcgcctttt tccgatctga cgagaaagtc cgtagcagcg cacctgcccc gttagcgtat
     8521 gacaccacca gcgcatattg cagtaccttc ggatcttcaa tgcctgccag cgggccggtt
     8581 tccagaatat tcaggtaagc cgcccccatt gaaatattac gctctggatt tttcagctcg
     8641 ctggtcgtcg gctcaccact ccagcccata cggcgataaa catcacgtcc ggaggttgaa
     8701 gcttttaact gcatcaaacc aatggcattc gatttactca ccgcgttcgg attaccaccc
     8761 gattcgatag cgataatcgc cgtgatcaat tgtggatcga cgccccaggc tgcaccggct
     8821 ttctggctta ttggcatcca ctgcatcgca cgttgcaccg gaactttcgc gttccacggc
     8881 gggttcgtat agtcatgctt tgatgaacaa cccgctaata acacaatcaa aaaggcaaac
     8941 catctcaatt tcacttcttc tatccttata gcctggaatc gccgttgagg tgacaacggc
     9001 acacctaagc ggcatgatat acatttcgtt tctgttgtca gcaaggaatt gccatgtctc
     9061 tgtttcactt aattgcccca tcgggttaca gcattaaaca gcacgccgcg ctgcgtggta
     9121 tccagcgcct taccgacgcg gggcatcaag tcaataacgt cgaggttatt gcccgtcgtt
     9181 gtgagcgttt tgcgggtacg gaaacggagc gtctggaaga tctcaattcc cttgccagac
     9241 tgactacccc caacaccatc gtgctggctg tacgcggcgg ttacggtgcc agtcgtttac
     9301 tggcagatat tgactggcag gcactggtgg ctcgccaaca acatgacccg ttactcattt
     9361 gcggacatag cgattttacc gccattcagt gcggtcttct ggcgcaaggc aatgtcatca
     9421 cctttagcgg ccccatgctg gtagcaaact ttggtgcgga tgaactgaac gcctttaccg
     9481 agcaccattt ctggctggcg ttacgcaatg aaaccttcac cattgaatgg caaggggaag
     9541 gcccgacatg tcagaccgaa ggcacgttgt ggggaggcaa tcttgcgatg ctgatttcac
     9601 tgattggtac accgtggatg ccaaaaattg agaacggtat tctggtgctg gaagatatta
     9661 acgtgcaccc tttccgcgtc gaacgtatgc tgttgcagct ctatcatgcg ggaattttgc
     9721 cacgtcagaa ggcgattatt ctcggtagct ttagcggcag cacgcccaat gattatgacg
     9781 caggttacaa cctggagtca gtgtacgcgt ttttgcgttc ccgcctgtcg attccactca
     9841 ttaccggcct cgattttggt catgaacaac gcacggttac gctgccgttg ggcgcacatg
     9901 ccattctgaa taacacccgg gaaggcgctc agttgacgat tagcggtcat cctgttctta
     9961 aaatgtaaaa aaatcacgct tcagggctaa gtaaaacgct gtctctgccg ctaatatggt
    10021 agggtttata taagagacag cgtaatcagg agtaaccgac cattggatgc cacaacaata
    10081 attagccttt tcatcttagg atccatcctt gtcaccagca gtatattact tagttcattt
    10141 tcttcccgtc ttggcattcc tattctggtt atttttctgg cgatcggcat gctggcggga
    10201 gtcgatggcg tcggcggtat cccgtttgat aattacccct tcgcctacat ggtaagtaac
    10261 ctggcgctgg cgattatttt gctcgacggc gggatgcgca ctcaggccag ttcctttcgt
    10321 gtggcgttag gaccggcgct gtcgctggcg acgctgggcg tacttatcac ctctggttta
    10381 accggcatga tggcggcgtg gctgtttaat cttgatttga ttgaaggctt attaatcggc
    10441 gctatcgtcg gctccaccga tgctgcagcg gtcttttctc tgctgggtgg taaggggctt
    10501 aacgaacgtg ttggctcgac gctggaaatt gaatccggca gtaatgatcc aatggcggtc
    10561 tttctgacga ttaccctgat tgcaatgatc cagcagcatg aaagcagtgt cagttggatg
    10621 ttcgtggtcg atattctgca gcaatttggc ctcgggattg tcatcggtct tggcggtggt
    10681 tatttactgc tgcaaatgat taatcgcatc gccctgcctg ccggattata tccattgctg
    10741 gcattaagcg gcggtatttt aatttttgct ttaaccacgg cgctggaagg cagcggtatt
    10801 ctggctgttt atctgtgcgg ttttctgctg ggtaatcggc cgattcgcaa ccgctacggc
    10861 atcctgcaaa atttcgacgg cctcgcctgg ctggcgcaaa tcgccatgtt cctggtgctg
    10921 gggctattgg ttaacccaag cgatctgctg cctattgcca ttccggcgct cattttgtcg
    10981 gcatggatga tattcttcgc ccgtcccctt tcggtatttg ctggattgct ccctttccgc
    11041 ggtttcaatc tgcgtgagcg cgtgtttatc agctgggtgg gattacgcgg cgcggtgcca
    11101 atcatcctag ccgtgttccc gatgatggcg gggctggaga atgcgcgact gttctttaat
    11161 gtcgccttct ttgtggttct ggtttcactg ctcttgcagg gaacatcact ctcgtgggcg
    11221 gcgaaaaaag ccaaagtggt ggttccgccc gtcggacgtc cggtgtcacg cgttggcctg
    11281 gatattcatc cggaaaatcc gtgggagcag tttgtttatc aattgagtgc tgataaatgg
    11341 tgtgtaggtg cggcactgcg cgatttgcat atgccaaaag agacgcgtat tgcagccctg
    11401 tttcgtgata accagttgct tcatcctacc ggcagcaccc gactgcgcga aggcgatgta
    11461 ttgtgtgtaa ttggtcggga acgcgatctc ccggcgctcg gtaaactgtt cagccagtcg
    11521 ccaccggtcg cgctggatca acgcttcttt ggtgacttca ttctcgaagc cagcgccaaa
    11581 tatgctgatg ttgcgctgat atatggcctg gaagacgggc gagaatatcg tgataaacag
    11641 caaacgctgg gtgaaattgt ccagcagttg ctaggcgcgg caccggttgt cggtgaccag
    11701 gtagagtttg ccgggatgat ctggacggtg gccgagaaag aagataatga agtgttgaag
    11761 attggcgttc gggtggctga agaggaagct gaatcttaaa tttaatgcag catctatcgc
    11821 cggatgcgat gctttactga acgcagcctg agcctgtaat aaacttgtca tttcctgatt
    11881 ttgatatgct catcccaaca tcagaagaag gttaaattat ggatgacaaa ccattgcagg
    11941 ctctggctaa tgaactcaaa acaccggctc aagaaagtca gcgtaagggc tttttccgac
    12001 tgtaacaggt aataaaaacc agccccgcat taccggtcaa ccatcagggg cccctgcctg
    12061 acatcgcctg aacatattcc cccacgcaac ctgccgcact gacatagccc acacaatagg
    12121 cgtttcatcc gcctgtagca ccggatgcat gagcgatttc ttatagttat tcctttgctt
    12181 aataacgatt atttccgggt cgttcactct cgtattttct ttaatacgtc agagctacct
    12241 aggccgtaat acagcatgta ccagacccga cacggcattt ctgaccagga aaaaagtcaa
    12301 cgatgagtag tgatatcagc gcaaatgacg tcctgtggct cctttgcggt gaccacattc
    12361 acgtctccgg cgctgccaga ggatatttat ttcaaggaaa cgtagcatta tggctagctt
    12421 taaagacatc tgcccattcg cccccgccat tcccccatct tccgttaacc caggcgtacg
    12481 acgcccgcca gggcggctct tctcaagaat caaattttgc agaacatgaa gtatctcagc
    12541 ccgcaaaaat tcagctgggg tgatgccccc tggcagatca ttgacctcag tatcgcgggc
    12601 aaagtgaaca tacaggtgga caacaatacc atcatcacgt tggggactcg tctgaatcag
    12661 caacataacg agttcatgat ggtcgccaaa tggtgcgaat gggctataca gcaggatggt
    12721 ttgcaagaaa acctgcaaaa aaacctgtac gaaatcctgg aagaaaacca gcaaaacaaa
    12781 cagtcagaaa ttccccaaga agacctgaaa gaaagtctgg aagaaatcaa ggaaaatatc
    12841 ctggaagaaa acctgccagc aagccggatt gaaaaccggg cagaagcatt gcgcagaatg
    12901 aaggaatgtc tcattacaag acggagtatg ctcaatctta gcaacctggg attgacttca
    12961 ctccctgaga atttgcctcc acatctgatt gaattttact gcagtaaaaa cgtgttgacc
    13021 gcgttaccga aggtgatgcc aaagtggctg ctggtgcttg actgtacaga taacgtcttg
    13081 atcttgttac cgaaggtgca gccctcaaaa ctgatggtac tgaagtgcta tgaaaacagt
    13141 attatctggc tgcctgagct gtcgactaac ctgagagtga ttaactgttc tgaaaacttc
    13201 ttgcaatttt taccgccgtc gatgccccag tacctgtata aactatcctg tgccggcaac
    13261 aatattaact caatacctga tgagatgctg gagaacttga ctcgcctgaa agtatttgac
    13321 tgttctagta acgatttgat ctcttcacca cggctgccgc ccaaactaat aatttactac
    13381 tgtggagaaa acaagtttaa aacggtacag gtgccgcagc cccagagcct gaaggtgttt
    13441 gactgtaatg gtaacccgtg ggacaaagac aatttaccga cgctgctcaa agccgtcgag
    13501 ggcctgaaaa aacagcaggg cctgaaggac cttttggact ttttgcacaa ggaaggctag
    13561 gttgacctgg aaggactcga ggagctggag gaccttatgg atctggagtt cctggatgac
    13621 ccggaactcc tggagcgcgt gaaggtacag gaggacctgg agctcctgga tcaacagttg
    13681 ggcctgttga gtctggaaag acaacaggac tcgcagcctg ttaatcaaca atctgaacat
    13741 gaacccgaat ctgcatcaag ggtgaagcgt gatttatctg aagtcgactc cgagtcaaca
    13801 atgaagcgta agcgttttat gtagaacgcc tgtgcggcga gttcaggttc cggggaaata
    13861 gtgtccattc ttttaatgga cactatcatg ggcaatggct ctgtcacatt aacaactaca
    13921 gttgctgacc agccggatgg atgatttttt cgcaaccaga agacgctgcc tccgatccgg
    13981 cttacaacaa gttacactgt cacaaccggg acgcgtaacg ccagtgcgca catcaactca
    14041 tagcccaccg ttccggcagc ggcggcgaca tcatcaattt tgatctcctt gccccacagc
    14101 tcaaccggcg taccaatccc cgcctgcggg caaggcgtta aatcaaccgc cagcatatcc
    14161 atcgagacgg tccccaccgt catggtgcgc acgccgtcca ctaaaacagg agcaccggta
    14221 ggcgcatggc gcggataacc gtcggcgtat cctgcggcga caatgccgat tcgctgttca
    14281 tcgcgcgcag tatagcgacc gccgtagccc acacgctcgc ccgcttttag cgtctggaca
    14341 ccaataatct cactgcttag cgtcatcacc ggacgtaatc cggtattggc gatatcacgc
    14401 cactgaccgg acggcgaagc gccatacaaa ataatgccag gccgaaccca gtcaaaatgc
    14461 gcttccgggt gccacagagt cgccgccgaa ttggacaacg aacgccgaca ctccagcccc
    14521 tccgccgcct gctcaatacg cgccatcgcg ccggaaattc catcaggatg ttccgcttca
    14581 gcaaaatgcg acatcagggt catttcgcca acattcgcca ttgcccgcaa ctgctgccag
    14641 acggtaagca cgcgatcgga ctggaagccc aaccgattca tcccactgtt cactttaaga
    14701 taaatatcca acggtgcttt tagccgcgca ttttgcagtg ctttgagctg ccagttgctg
    14761 tgtacgcagg tggtcaggcg gtgctggtca taaatctcca gatcctgagc atggaaaaat
    14821 ccttccagca tcaggatcgg ccccttccag ccgcgctcac gtaacgttat tgcctcttcc
    14881 aggttaagta atgcaaagcc atcggtggcc ccgagcgcgc tccagatacg ctcaatacca
    14941 tgcccgtaag cgttcgcttt taccaccgac cagacgcgcg cgtgcggcgc ggcctggcgg
    15001 acaatggaca gattctgttt taatgcctgc agatcgaggc tggcctgtat cggacgggtc
    15061 atctcgtttc cttagctgtg tgcgccatgt aaatggcccg gacgtgatgg cgtaaatcca
    15121 cggctgtagc gcgctacgct tagatcctca tatgggatcg ctggcgtgcg accagagagc
    15181 agatcgctta acaactgacc ggaaccgcaa gccatcgtcc agccgagcgt gccgtgaccg
    15241 gtattcagcc acagattttt aaagcgtgta ctcccgacaa ccggcgtacc gtctggcgtc
    15301 atcgggcgca aacccgtcca gaaagtcgcc tgctcgacat gaccgccgcg tggatagaga
    15361 tcgcgaacca ccatttccag cgtttcacga cgcggttgca acagctcggt gttaaaacca
    15421 acaatttccg ccattccgcc aacacgaatg cggttatcga aacgggtaat ggcgattttg
    15481 taggtttcat caagaatggt ggatactggc gcaccatctt cctgcgcaat tggaatagtc
    15541 agcgagtagc ctttcagcgg atacaccgga atatcaacaa tgcctttgag catcgccgtc
    15601 gagtaagaac caaacgccat cacatacgca tcggccttaa tcacttcatc gccaaacttc
    15661 acgccgtaga tttgctcgcc gtcgcaaagc agttggtcaa cgggcgtatt aaagcggaat
    15721 ttaacccccg cctgctctgc catccgcgcc agattctggg taaacagctg gcagtctccg
    15781 gtttcatcat tgggtaactg caggccgccc gtcagtttgt gcgctacttc tgccagcgcg
    15841 ggatccactt ccgccaggcg gctggattcc agcagctgat acggtacgcc ggcatcttcc
    15901 agcacggcga tatcgcgggt cgcattttca tactgttgtt cggtacggaa cagttgcagc
    15961 gtcccaccct gacgcccttc atactgaata ttggtttcgg cgcgcaatgc tttcaggcaa
    16021 tcacggctgt attccgccag acgcaccatc cgccctttgt tttccatgta gtggctggtg
    16081 tcgcagttac gtaacatttg ccacatccat ttcaactgaa actgcgtacc gtcgagacga
    16141 accgccagcg gcgcatggcg ctggaacatc catttaatcg cttttaaagg cacacctggt
    16201 gctgcccacg gtgcagcata tccgggggag atttgcccgg cattcgcggc actggtttcc
    16261 agggcggccc ccggctcccg atcaatgacg gtgacctcat gtcctgcctg atttaagtac
    16321 caggcgctgg caacgcctac cacaccactt cccagtatga caactcgcat agccactccg
    16381 ataacggtag atacagtaaa agaataataa tctaattaca tcttgataac tcagttgaaa
    16441 atattattca acatacggct tttttatggt gagctgactc acatctccct gactctgcgg
    16501 caatatggcg tctttagtat ctcctgctga acgccctttt tatccagcat aaaattccgt
    16561 tcagaagcgg attagtggca ctctgataat aagaaatcgc aaagaaaaaa attctccggc
    16621 gacgcggttt ttaacaggtg ttctatgctt gaaatgaggt gctccacaca gagagcgccg
    16681 acaacaatga ggtgcgcgta tggcgacgat cgattctatg aataaggaca ccacacgttt
    16741 gagcgatgga cccgactgga cgttcgacct gctggatgtt tatctggcag agatagaccg
    16801 ggtggcgaaa ctctaccggc tggataccta cccgcaccag attgaagtga taacctcaga
    16861 acagatgatg gatgcctatt ccagcgtcgg catgccaatt aactatccgc actggtcatt
    16921 cggtaaaaag tttatcgaga ctgaacggct gtataagcac ggtcagcaag ggctggccta
    16981 tgaaatcgtc attaactcta acccgtgtat cgcttacttg atggaagaga acaccattac
    17041 tatgcaggcg ctggtgatgg cacacgcctg ctatggacat aactctttct ttaaaaacaa
    17101 ttgcttattc cgcagttgga ccgacgccag ttcgattgtc gattatctga tttttgcccg
    17161 taaatatatt accgagtgcg aagaacgtta tggcgttgat gaagtagaac ggcttctgga
    17221 ctcgtgccac gcgctgatga actacggcgt ggaccggtac aaacgcccgc aaaaaatctc
    17281 gctgcaagaa gagaaagccc ggcagaaaag tcgcgaagag tatctacaaa gtcaggtcaa
    17341 tatgctctgg cgtaccctgc cgaagcgcga ggaagagaaa acggttgctg aagcgcgccg
    17401 ctatccgtcc gaaccacaag aaaacctgct ctattttatg gagaaaaatg cgccactgct
    17461 ggaatcatgg cagcgtgaaa tcctgcgtat tgtgcgtaag gtgagccagt atttttatcc
    17521 gcaaaaacag actcaggtga tgaacgaagg ctgtgcgacc ttctggcact ataccatcct
    17581 taaccatctg tatgatgaag ggaaagtaac ggaacgtttt atgctggagt ttttgcacag
    17641 ccacaccaat gtggtcttcc agccccccta taacagcccg tggtacagcg gcatcaaccc
    17701 gtatgccctc gggttcgcca tgttccagga tattaaacgg atttgtcagt cgccaacgga
    17761 agaagacaaa tactggttcc cggatatcgc cggttccgac tggctggaaa cgctgcattt
    17821 cgcgatgcgt gatttcaaag atgagagttt tatcagccag tttctgtcac cgaaagtgat
    17881 gcgtgatttc cgcttcttca ccgtgctgga tgatgatcgg cataattatc tggagatttc
    17941 cgctattcat aatgaagaag gttatcggga gatccgtaac cggttatcgt cgcaatataa
    18001 cttaagtaat ctggagccga atattcagat ctggaacgtg gatttgcgcg gcgaccgttc
    18061 gctgacgctg cgttatattc cacataatcg cgcaccgctg gatcgggggc gcaaagaagt
    18121 cctgaagcat gtgcatcgcc tgtggggatt tgatgtgatg ctcgaacagc aaaacgaaga
    18181 cggcagcatc gagttgctgg aacgttgccc gccaagaatg ggaaatctgt aaaaacaaca
    18241 aaaaacccct cgtttgaggg ggttgctctt taaacggaag ggattatcgc ccctgaatgg
    18301 ctaaatcacc cggcagattt ttctgcatcc ggtgccaaat ctcgccactc tcatgcccat
    18361 agcgacgcac tgtttcgtac acctgatcgt gcgcgccttc actgcacaac gccgacagtt
    18421 tgtggtagaa gcccagcgcc agactgcgcg cttccggatt ggcgaaatag tgacgaccaa
    18481 tgcgcgtata cagccctttc atcccgttaa ggatcagacc gtaaatcggg ttgccggaag
    18541 caaacgccag gccgcggaat atgttgtaat ccagctcggc aaaggcatcg gcgtgatcgg
    18601 ccacttcatt agcggtagcc agcacttcct gcgctttatc gggatgctga cgaaacgcgg
    18661 tgcgaataaa aatagtggaa atattggtac gcaccgacag caaattatca ataagctgcg
    18721 gcacactttc gtgatccagt cgcgccagtg tttcaaggat atttaaaccg gaagtttccc
    18781 agaaattatt caccttcgtc ggcttgccat gttgaatggt caaccagcca tctcgtgcca
    18841 gacgctgtaa cacttcacgt aacgtagtac gcgttacgcc aattaattct gaaagttcac
    18901 gttctgcggg caaaatagtc ccgggaggga agcggttatt ccagatactt tcaataatgt
    18961 actcttccgc gaaacccgcc gggctttgcg ccttaatgac catagtgaga tttccataac
    19021 acagcaaaac aaagttggac tcatcatacc agagggctaa ctacgctgat agcagacaaa
    19081 aagaagaaaa agggatcaag gcttcatttt ccgcatctct ttccccttga ctatcaagac
    19141 ttgcctcact cctgcctgac cgttaagctt tgcgtttaaa cataaaaaca tcattgatta
    19201 tttgtaaggt aagggaaacc atcatggaga tctcctgggg ccgcgcgcta tggcgcaatt
    19261 ttttgggcca gtcccccgac tggtacaaac tcgccctcat cattttctta atcgtaaacc
    19321 cgttaatttt cctcatcagc cctttcgtcg cgggctggtt gctggtagcg gaatttattt
    19381 tcactctggc gatggccctg aaatgctacc cgctactccc cggtggtctg ttggctatcg
    19441 aagcggtatt catcggcatg accagcgcgg aacacgtccg tgaagaggtg gcggcaaatc
    19501 ttgaagtctt gctgttattg atgtttatgg tggcgggtat ctattttatg aaacagctgt
    19561 tgctgttcat atttacccgt ttgctgttaa gcattcgctc caaaatgctg ctgtcgctct
    19621 ctttttgcgt ggcggcagcg ttcctctccg cgttcctcga tgccttaacc gtcgtggcgg
    19681 tggtgatcag cgttgcagtc ggtttttatg gtatttatca tcgcgtagcc tcttcccgta
    19741 ccgaagacac cgacctgcaa gacgatagtc atatcgacaa gcattacaac gtggttctgg
    19801 aacagttccg tggctttctg cgtagcctga tgatgcatgc cggtgtcggc accgcattag
    19861 gcggcgtaat gaccatggtg ggcgaaccac agaacctgat catcgctaaa gcggctggct
    19921 ggcattttgg cgatttcttc ctgcgcatgt cgccggtgac cgttccggtt ctgatttgtg
    19981 gcctgttaac ctgcctgctg gtagagaagc tgcgttggtt tggctacggt gaaacgctgc
    20041 cggagaaagt ccgcgaagtg ttgcaacagt ttgacgatca aagccgccac cagcgtaccc
    20101 gtcaggataa aatccgtctg attgtccagg cgattattgg cgtctggctg gtgactgcgc
    20161 tggcgttgca tctggcggaa gttggcttga ttggtttgtc agtcattatt ctggcaacat
    20221 cattgaccgg tgtcaccgat gaacatgcta tcggtaaagc cttcaccgaa tctctgccat
    20281 tcaccgcact gttgacggtg tttttctcgg tagtcgcggt gattatcgac caacaactgt
    20341 tttcgccgat tattcagttt gtgttgcagg catctgaaca tgctcagctg tcgctgttct
    20401 atattttcaa cggtctgctg tcatccattt cggataacgt cttcgtgggg acgatttata
    20461 tcaacgaagc gaaagcggca atggaaagtg gcgctatcac gttgaagcaa tacgagctgc
    20521 tggcggtcgc cattaatacc ggtaccaatc tgccctctgt cgctacgccg aacggtcagg
    20581 ctgcgttcct gttcctgctg acctctgcac tcgcgccatt gattcgcctc tcttatggcc
    20641 gcatggtgtg gatggccctg ccttacaccc tcgtactgac actcgtcggc ttgctctgcg
    20701 tcgagtttac gcttgcccct gtaaccgaat ggtttatgca aatgggctgg atagcaacgc
    20761 tttgataaca acttaccggg catataaatg cccggtttgc cttttcgccc gataattgtc
    20821 caattgcgtc ctatttttgc tgcctcctgg tggcgcagcg aatgaattgg tttaaactgc
    20881 gcactctatg catattgcag ggaaatgatt atgttgcgat ttttgaacca atgttcacaa
    20941 ggccggggcg cgtggctgtt gatggcgttt actgctctgg cactggaact gacggcgctg
    21001 tggttccagc atgtgatgtt actgaaacct tgcgtgctct gtatttatga acgctgcgcg
    21061 ttattcggcg ttctgggtgc tgcgctgatt ggcgcgatcg ccccgaaaac tccgctgcgt
    21121 tatgtagcga tggttatctg gttgtatagt gcgttccgcg gtgtgcagtt aacttacgag
    21181 cacaccatgc ttcagctcta tccttcgcca tttgccacct gtgattttat ggttcgtttc
    21241 ccggaatggc tgccgctgga taaatgggtg ccgcaagtgt ttgtcgcctc tggcgattgc
    21301 gccgagcgtc agtgggattt tttaggtatg gaaatgccgc agtggctgct cggtattttt
    21361 atcgcttacc tgattgtcgc ggtgctggtg gtgatttccc agccgtttaa agcgaaaaaa
    21421 cgtgatctgt tcggtcgcta atccattcgg cgctcctgcg ggagcgcttt tttcctgccg
    21481 ctatatttat ttgacccgca gtaaatcaga acttcgcgtt gtataacgtg gtgaaagcat
    21541 agctcgcttc atctgccatt gttgttggat cccctgcccg gcaaaataga gtgttcctct
    21601 gccctcttta gcattcagag tatccattac cgccatcaat tgctcactcc cggggcgcgg
    21661 tgcgttgtca tcgaataaat tgagctgcgc gactccctga ctgaagaaat cccccagcat
    21721 cacgcccgct ttttggtagc gatggcccgc ttgccagatg gcatccagag atcgcgtagc
    21781 agcgttaatg atatccctgc tgtcctgagt gggcgtcagc agttttaccg acgcgctatt
    21841 gccgtaataa ggttcattga gcgcaaatgg tgacgtctta ataaatgtgg agataaaccg
    21901 acaatattga tgctcactgc gaagtttttc cgccgcccgg gcagcgtaac tacaaatggc
    21961 ctgccgcatc gacgtataat ccgtgatgcg ttcaccaaac gagcgggaac agataatttc
    22021 ctgcttcgtc ggtgcaaact cttccagttg caaacagggt tcgccgcgca gttcacgcac
    22081 cgttctttcg agcatgacat taaaatgttt acggataaac cggatatctg tatccgccaa
    22141 atcgagaacg gttttgatcc ccatcgcgtc cagttttttg ctgatccgcc gtccaatccc
    22201 ccagacgtca tccacgggga gagcagacat taatttacgc tggcgttcca gatttgataa
    22261 atccaccacc ccacccgtct gccgctgcca tttttttgcc gcatgattgg caagcttagc
    22321 caacgttttg gtctgggcga tccccacacc aacagtaaga tgggtacgtt gtagcaccgt
    22381 tgcgcgaatt tctctgccaa aatcagtcag atcgcgacaa ttacgcacac ctgtcagatc
    22441 gcagaatgct tcatcaatac tgtaaatctc gacgcggggc gatagttctt ccagcgtcga
    22501 catcacccga ttgctcatgt ctgcgtaaag ctcataattg ctgctaaagc aaaccacacc
    22561 acagcgacga aacagatctt tttgtttgaa ccagggatcg cccattttaa cgcccagcgc
    22621 ctttgcctca gcgtttcggg cgataacgca accgtcatta ttcgatagca caaccaccgg
    22681 tttaccccat aaatcagggc gaaacaccgt ctcacagctg gcataaaacg cgtttacatc
    22741 acagagggca aacatcagcg catcgcctta acgacgtgga tcaccacacc aaagacatcc
    22801 agcgtatctt cgctactgat ggtaatgggc gagtacgcac tgttcatggg aataagctgt
    22861 accgtcgggc gtagttgcaa ttttttcacc gtaaactcgc cgtcaacagc agcgatgaca
    22921 atatcaccat ggctggcggt aatagcacta tcgacaatca gtaaatcacc gtcactaatt
    22981 ccaccatcaa tcatagaatc accacttgct ttgacgaagt aagtcgcgct gggatgctgg
    23041 atcaacagtt gattcagatc gatgcgctgt tcaacgtaat ctgctgccgg tgaaggaaag
    23101 ccacactgaa caagatcgct aaatagcgga aaagtcacaa tttcgcggag atccgcaggc
    23161 ttgataaaca acataataat ctgcctgaag ttatactgtt tttatataca gtggtctgtt
    23221 cttgccagca gatcaatact gattcaggct atcaatattt gtcgctgcat agactgctga
    23281 tttttcgttc tcttatcctg tgctcacgtg gccttctggc gacgacgctc atccagcaga
    23341 aatgaaaaat atcacccggc aaaaaaatag aatagaagca ttcgccataa tgacattaaa
    23401 cattgtttga tatttatcat attaatagaa ataaagacat tgacgcatcc cgcccggcta
    23461 actatgaatt agatgaagta aaatttatta atagttgtaa aacaggagtt tcattacaat
    23521 ttatatattt aaagaggcga atgattatga ctgaaatcgt tgcagataaa acggtagaag
    23581 tagttaaaaa cgcaatcgaa accgcagatg gagcattaga tctttataat aaatatctcg
    23641 atcaggtcat cccctggcag acctttgatg aaaccataaa agagttaagt cgctttaaac
    23701 aggagtattc acaggcagcc tccgttttag tcggcgatat taaaacctta cttatggata
    23761 gccaggataa gtattttgaa gcaacccaaa cagtgtatga atggtgtggt gttgcgacgc
    23821 aattgctcgc agcgtatatt ttgctatttg atgagtacaa tgagaagaaa gcatccgccc
    23881 ctcattaagg tactggatga cggcatcacg aagctgaatg aagcgcaaaa atccctgctg
    23941 gtaagctcac aaagtttcaa caacgcttcc gggaaactgc tggcgttaga tagccagtta
    24001 accaatgatt tttcagaaaa aagcagctat ttccagtcac aggtagataa aatcaggaag
    24061 gaagcgtatg ccggtgccgc agccgatgtc gtcgccggtc catttggttt aatcatttcc
    24121 tattctattg ctgcgggcgt agttgaaggg aaactgattc cagaattgaa gaacaagtta
    24181 aaatctgtgc agagtttctt taccaccctg tctaacacgg ttaaacaagc gaataaagat
    24241 atcgatgccg ccaaattgaa attaaccacc gaaatagccg ccatcgggga gataaaaacg
    24301 gaaactgaaa caaccagatt ctatgttgat tatgatgatt taatgctttc tttgctaaaa
    24361 gcagcggcca aaaaaatgat taacacctgt aatgagtatc agaaaagaca cggtaaaaag
    24421 acactctttg aagtacctga agtctgataa gcgattattc tctccatgta ctcaaggtat
    24481 aaggtttatc acatttaaaa acacagacgt tttttaaaga ttgagagtta cctgttgata
    24541 ataaggtatc gggtaactct cattatgaca gagcgctttc aaaaacaggg ctacgaaatg
    24601 gcgagcatcc agacgtatat taagaaagcc cccatcagca tctattaaat ttatcactgc
    24661 gcccagtcag gtttatttaa gatatgatcc tgccagtcaa tcacttctga ttctttcact
    24721 gcgatatgac gcacagagat acgttcacca tgcattgccg cttcgaacca gtaagtagcg
    24781 gatgccacgc aggtaaatct ttaccttccg ccagcaaccg ataagcgcag gtcattggca
    24841 gccattcgaa tgttggcaga ttttcacggg ttaatttaat gcagtcgggt tcaaactcga
    24901 aacgacgttc gtagttccga cattgacagg ttttaatatt gagctggcga caggcgacgt
    24961 tagtgaagta gatttcgtcg gtgtcttcat ccatcagttt atgcaagcaa cactgaccgc
    25021 aaccatcaca caacgactcc cattccgcat cgctcatttc gtccagggtt ttactttgcc
    25081 agaaaggtac atcgctcatc aggtgttccg ccattacgtt aaaacgcacc ttataaccag
    25141 tctggcacag cgatgcaagt tttgccgccg ctttctggcg gcaaaaagta ttacaaaacg
    25201 cgagttgtca aagaatgccc atcgaaagtg actgtcagct catcaccgct ttgcaacggg
    25261 ccgacgccat caggcgtgcc tgtcaacaca acgtcaccgg ccttgagggt aaaaaacttg
    25321 ctcatataag cgatcaacgg aacgatttta tggatcatgt ccgcagtcgt accttgctgg
    25381 cgtttttcgc cgtttacgct caggcccagc gttgtatttt gtggatcgcc ggtaaattcc
    25441 gccgcgggga taaacccgga aagcggacaa gagttatcaa acgctttagc cttttcccac
    25501 ggctgcccgg ctttcttcat ttttccctga acatcacgca atgtcagatc aagcgccacg
    25561 ccgtaaccgg caatggcttt gcggacatgc tcttccgttg cctgacgcag cgtcgcgcca
    25621 atcaacaccg ccagttcgac ttcatgatga actgaaccga aatcggatgg gatcgccaat
    25681 ggctgccgca gatcgcacag tgccgtttct ggtttaataa acagcactgg ctcttcgggc
    25741 actgcgctgc ccatctcttt aatatgtttg gcatagttac tgccaacaca gactacttta
    25801 ctcaccggat aa
//
LOCUS       DAJTSI010000051        25559 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000051 DAJTSI010000000
VERSION     DAJTSI010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25559
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.051"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..648
                     /locus_tag="OO327_001848"
                     /pseudo
     CDS             1..648
                     /locus_tag="OO327_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163825.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            645..1031
                     /locus_tag="OO327_001849"
     CDS             645..1031
                     /locus_tag="OO327_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018409.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LTCWFHYKDGQWRVITYIFWPPGARF"
     gene            1056..3269
                     /gene="tssI"
                     /locus_tag="OO327_001850"
     CDS             1056..3269
                     /gene="tssI"
                     /locus_tag="OO327_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR6018410.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHESRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTPDVSE"
     gene            3292..3737
                     /locus_tag="OO327_001851"
                     /pseudo
     CDS             3292..3737
                     /locus_tag="OO327_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3759..4166
                     /locus_tag="OO327_001852"
     CDS             3759..4166
                     /locus_tag="OO327_001852"
                     /inference="COORDINATES: protein motif:HMM:NF017315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HCR6018411.1"
                     /translation="MYEAARVDDPIYHTSALAGFLIGAIIGIAIIAVASFAFFTCGFL
                     AGLILSFLADQIASGVLQLGEAIGRSIHSTVGKILTGSDNVSTNSRPAARAVLSTVIC
                     EDHSPEIRIAQGSGNIYINSQLPVRMTTLSVMQ"
     gene            4163..6451
                     /locus_tag="OO327_001853"
     CDS             4163..6451
                     /locus_tag="OO327_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6018412.1"
                     /translation="MIEDGSPNVFLGGGTQTVLEISPEIPDWLRQVVDVLYVVAGMLG
                     GLAGAWRQAAKMGSKFGTKCAAKFIGGEMVGMAISDTVMGLFSNPVDVTTGQKILLPE
                     TDFTLPGRLPVTCSRFYASHLETEGLLGRGWRLNWEINLREDETYITFIGVQGRELSY
                     PKEMLIPGHQIFDPEEQFYLSRLHDGCYVLHYTDCSYYVFDEFDDHGVAPLLFMETPY
                     RQRIAFGRENGRLVRVASSSGHHLLLHRTMTQAGERLSHIELLKGGRPGNLVEYRYDD
                     NGQLTGVVNRAGVTVRQFAYENGLMTEHRNATGFTCTYHWEEIEGFPRVVEHTTSDGE
                     DYRFHYDFAGGQTVVTGRPEQKWQWWFDEETYVTAHRTPGGGMYRFTYNENHFPVAVE
                     LPGERRVTLEYDTLSRVVKETDPAGRVTQTQWNGSFAEITRRALDDDHVWKADYNEHG
                     QVIRETDPEGRVTRYGYDDQGLPETVCHPGKQQDRYTWNALGLLSSHRRITGSVQSWQ
                     YTQRGMLARHTDEEKRETRWQYTPEGLVASLSNGNGAQYRFSYDGDGRLTGEQRPDGL
                     IRMFALNADGFPVIIPTQGTEGGVRNEQQERDALGRLLRSDTQHSTRTFSYNRLDQIT
                     EVTLTPTEEGERLHHMQADTVRFAYDRSGWLTAEHSVHGSIKYRRDALGNPTDITLPD
                     GQHLSHLYYGSGHLLQTALDGITVSEYERDSLHRQVIRTQGKLATFSGYNADNRLSWQ
                     RSLPGGSQNPQQAVLPRRRTTA"
     gene            6448..7437
                     /locus_tag="OO327_001854"
     CDS             6448..7437
                     /locus_tag="OO327_001854"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018413.1"
                     /translation="MTARDYIWNADGEVGGINDKLRGCLVFSYDRSGWLTSRTGQMYD
                     HDHYYYDKAGNLLTDEYQGAVMDNRLPGYGRDRYRYNEWGELTERRDQQLEWNAQGQL
                     TRVISSNSETRYQYDALGRRISKATSNLHTDRGERSRTTFVWEGFRLLQETTWQGKRT
                     YLYDAEQPYTPVAAITGRGESQKIWYYHTDLTGTVHEVTAPDGTLVWAGYQAGFGENR
                     GDISNSGAYFEQPQRLPGQYFDEETGLHYNLFRYYAPECGRFVSQDPIGLNGGLNLYA
                     YAPNPLGWIDPLGLNSLGIDSNNIFRGDGNYKGGGIGQPKSGTISAQDIIDHL"
     gene            7537..7740
                     /locus_tag="OO327_001855"
     CDS             7537..7740
                     /locus_tag="OO327_001855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018414.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            7758..8078
                     /locus_tag="OO327_001856"
     CDS             7758..8078
                     /locus_tag="OO327_001856"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018415.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(8144..8376)
                     /locus_tag="OO327_001857"
                     /pseudo
     CDS             complement(8144..8376)
                     /locus_tag="OO327_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(8486..9256)
                     /gene="yafV"
                     /locus_tag="OO327_001858"
     CDS             complement(8486..9256)
                     /gene="yafV"
                     /locus_tag="OO327_001858"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR6018416.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            9437..9883
                     /gene="ivy"
                     /locus_tag="OO327_001859"
     CDS             9437..9883
                     /gene="ivy"
                     /locus_tag="OO327_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR6018417.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(9926..12370)
                     /gene="fadE"
                     /locus_tag="OO327_001860"
     CDS             complement(9926..12370)
                     /gene="fadE"
                     /locus_tag="OO327_001860"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCR6018418.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            12610..13188
                     /gene="lpcA"
                     /locus_tag="OO327_001861"
     CDS             12610..13188
                     /gene="lpcA"
                     /locus_tag="OO327_001861"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR6018419.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            13394..14161
                     /locus_tag="OO327_001862"
     CDS             13394..14161
                     /locus_tag="OO327_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR6018420.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(14132..14872)
                     /locus_tag="OO327_001863"
     CDS             complement(14132..14872)
                     /locus_tag="OO327_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR6018421.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            15256..15777
                     /locus_tag="OO327_001864"
                     /pseudo
     CDS             15256..15777
                     /locus_tag="OO327_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            15953..16450
                     /gene="rayT"
                     /locus_tag="OO327_001865"
     CDS             15953..16450
                     /gene="rayT"
                     /locus_tag="OO327_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCR6018422.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(16667..18379)
                     /locus_tag="OO327_001866"
                     /pseudo
     CDS             complement(16667..18379)
                     /locus_tag="OO327_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            18351..19136
                     /gene="lafU"
                     /locus_tag="OO327_001867"
     CDS             18351..19136
                     /gene="lafU"
                     /locus_tag="OO327_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR6018423.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            19207..20262
                     /gene="dinB"
                     /locus_tag="OO327_001868"
     CDS             19207..20262
                     /gene="dinB"
                     /locus_tag="OO327_001868"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR6018424.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            20314..20607
                     /gene="yafN"
                     /locus_tag="OO327_001869"
     CDS             20314..20607
                     /gene="yafN"
                     /locus_tag="OO327_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HCR6018425.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            20610..21008
                     /gene="yafO"
                     /locus_tag="OO327_001870"
     CDS             20610..21008
                     /gene="yafO"
                     /locus_tag="OO327_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HCR6018426.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            21018..21470
                     /locus_tag="OO327_001871"
     CDS             21018..21470
                     /locus_tag="OO327_001871"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6018427.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            21716..22759
                     /locus_tag="OO327_001872"
     CDS             21716..22759
                     /locus_tag="OO327_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR6018428.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            22821..23435
                     /locus_tag="OO327_001873"
                     /pseudo
     CDS             22821..23435
                     /locus_tag="OO327_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(23492..24949)
                     /gene="pepD"
                     /locus_tag="OO327_001874"
     CDS             complement(23492..24949)
                     /gene="pepD"
                     /locus_tag="OO327_001874"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR6018429.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            25211..>25559
                     /gene="gpt"
                     /locus_tag="OO327_001875"
     CDS             25211..>25559
                     /gene="gpt"
                     /locus_tag="OO327_001875"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6018430.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKP"
BASE COUNT         6323 a         6366 c         6846 g         6024 t
ORIGIN      
        1 acaaatattg atggcaatat cattatacat aaggatggta ggaggacgta tttattaaat
       61 caaaatactg gttcgattac ggctgtgata agatggaagt atgagtagta taaggataat
      121 accgttacag atgactggac tatatgggaa aaagctattt ttatgcgagc gatatattta
      181 tctgttcagc aggcatggaa tgggaaaata acttattctg tttccggtga atctgagttc
      241 gctaaaaaat ttcagggtaa agctttgcct tttgatgtaa gaataatttc tgcatctcag
      301 aatgaagact ggttggttat tgcaacaaag gttttgcctg gtgctgattt aagaacatat
      361 gttgatttta aaaacagtac ggttcatgtt gattcagcgg gcctggaaaa agtagcgaaa
      421 tgtattaact gtaataatac gttgcaagta aatatacctc atgaagccgg acatgtactt
      481 ggttacttag atgatgatta tgattcttcg tctccgtatg ttggagatat tagtgggttg
      541 atgaatgtgg gaatggagct ttgggaacga taccttaaaa acgcaactat aacgttgaat
      601 atcataatgc ctgaaactaa atttacttta ttgaatgtaa ccaaatgaaa tttttctgta
      661 ttatagtgat tttcctttca ataaattaca tagctatagt gaatgttttc aagtcttctg
      721 cagttgctca atccaccaaa tattatgaag aaaatgatat acatgatgga agtaatgaca
      781 tcttttttat gaagaaatat gggccgcctg ctcatgagtt cgtgaggcca cgatataaat
      841 ttgttggtga gtattatatc ggcttgaatc aaacttatcg cataacagat cctgaatttc
      901 aagatgttcc tattaaggaa atgttctgga atttgcaggg ggatttgaat ctgacatgtt
      961 ggtttcatta taaagacggg caatggagag tgattactta tattttttgg ccaccagggg
     1021 ctagatttta atccggtatt aaaggagcaa atatcatgag ttttgtatct acgggtaata
     1081 aaccagttgg aaacggtgga cccgtcatca ccacaccacc aatagctggc gaaagtggcg
     1141 gtatgagcac aggttcagct gtaaccgatg tatctggtgc cgccgaggag atggcagaac
     1201 aggctgcggc ggacctgttt ggtgcgctgc cggaaccgtc aggtctggtt aaagccgcag
     1261 tagctgctgc gcaggctgcc gctgcagcag cagggatctc ggatatggct ggtgcagttc
     1321 aggatgcagc ggcgtcgctt gcagccggtg ctccgggggc gcataacgtc acagtaagtg
     1381 gtagtgcggt gccgccgcag atgcttttgt ttgcaggcat gaatggttcg gaagggcttg
     1441 ggaatctgtt ttcttacaca gtacagctta aaacgctgga cgccctgaac ctggggtacg
     1501 tttcccccgc tgctaacctg ccgcttcagc tcatggtggg caaagacctg tgcgtcagta
     1561 tcgaactgga cggcggcgga aaacggtata tcagcgggct ggtgacggcg gcgagggtgg
     1621 tcggccatga gagccgttcg gtaacgtatg aattacgcat tgagccatgg ctgaagctgc
     1681 tgacccatac cagcgactac aaagcgttcc agaacaaaac cgtggtggat attctcgatg
     1741 aagtactgga tgaatattcc tttccggtgg aaaagcggct ggtggaaaat tatccacccc
     1801 gcgcctggca ggtgcagtat ggtgaaaccg acttcgattt catccagcgc ctgatgcagg
     1861 aatgggggat ttactggtgg tttgagcaca gcgagaacag ccacacgctg gtgctggtgg
     1921 atgccatcaa tggtcataaa gcgtgccctg actcgccgct ggtggagtgg caccaggaag
     1981 ggctgaaact ggataaggaa tttatccaca ccattacggc cagtgagagg ctgcgtactg
     2041 gaaagtgggt gatggacgat tttgatttta tgaagcctcg ttcgctgctt aaaagtaccg
     2101 tggccagtcc gcgtgatacc ggtcatgctg aatacgagca ttacgaatgg cctggtgatt
     2161 acttcactac gggcgaaggc gagatgctga cgcgcattcg tatggaagcg cagcgcagcc
     2221 caggcagccg ggcacatggg gccggtcata tccgtaccct catgaccggg tacaccttta
     2281 cgttgatgaa tcacccgacg gcagaaatta atcaggaata tctgctggta cagaccactc
     2341 tgtttttacg ggataatgcg cagcacagcg gtcaggatca gcattttacc tatgtcacca
     2401 cgtttgaact gcaccccacc agcgaagttt accgcccaca acgaaccctg agcaaacccc
     2461 acaccaaagg cccgcagagc gccatcgtca ccggcccggc ggggcaggaa atctggacgg
     2521 ataaatatgg ccgggtgaag gtgcagtttg gctgggaccg ttacggaaag aatgacgaaa
     2581 acagttcctg ctggatccgc gtcagctatc cgtgggcggg taaaggattc ggcatgatcc
     2641 agatcccgcg tattggccag gaagtactgg tggatttcaa aaacggcgat ccggatttgc
     2701 ccatcatcgt ggggcgcacc tataaccagg acaccatgcc gccgtggggg ctgccgggca
     2761 tggcgtcgca gagtgggata ttcagccact cattgcaggg agggccgact aacggaaata
     2821 tgctgcgctt cgatgataaa acgggtgcgg aagaggtgaa gttccatgcg gaaaaagacc
     2881 tcaataccac ggtaaaaaac aatgaaaccc acacggtcat ggtcgatcgc ataaaaacca
     2941 ttgtcaaaaa tgaaaccatc agcgtgggag aagaccgtaa tgccaccatc acaaaaaatg
     3001 atggtctttc cgtcaaactg gctcaaacca tcaatgtcgg aacgacatat cgtctggatg
     3061 ttggcgatca atttaccctt cgttgcggca atgcagcgct tgtattacac aaggatggct
     3121 cgattgagtt ttgcggaaaa caactgttac tgcacaccag cgatgttatg caactcattg
     3181 gcagtggcat tgatatgaat ccggatggcg gcacagccat gactgcggat gatattgccc
     3241 cgttacctac ccccgacgtt tctgaataaa aaatataaaa ggaatttttc tatgaccagc
     3301 ctttaccgta ttcaggaggg ctgctttgcc ctgcctgaaa cttttcttga tcgcaccgtc
     3361 aatatcttcg tcccatcggg caatgaaagg gcaactccca gtctgaatat tttccgcgat
     3421 acgctcaggc cagatgaaaa tctgaccacc tatattgacc gccagatagc cttgatgaaa
     3481 aaaaaaacct aggccagcac cgggtacttt cgcggggaac ggcactggcg ggaactggcg
     3541 aagctgccat gacaggtgaa cagattctcg caactcatcg ttctggaaaa acagaagtgt
     3601 accagcgtca ggccgggttt atcaccgggc cagcaaaggt actaatgttg acgctgacca
     3661 ctcagcgtcc ttttgatgat cacaccgacc agctatggac tgcatggttg actagttttc
     3721 agcctgctaa aagctaaaaa aaacagggag gttttgtcat gtatgaagcc gcacgggtgg
     3781 atgaccccat ctaccacacc agcgcccttg ccgggttttt gataggggcc atcataggta
     3841 tcgccattat tgccgtcgcc agctttgctt tttttacctg cggctttttg gccgggctga
     3901 ttttgagttt tctggctgat cagatagctt ccggagtcct gcaactgggt gaggctattg
     3961 ggcgctcgat acattccaca gtgggaaaaa ttctgaccgg ttctgataat gtcagcacca
     4021 acagtcgccc ggccgcccgg gccgtactga gtacggtgat atgcgaagac cattccccgg
     4081 aaatccgcat tgcccaaggt tccggcaaca tctacatcaa cagccagctg cccgtaagga
     4141 tgaccacact gagtgtgatg cagtgattga ggacggctcc cccaatgtct ttctcggcgg
     4201 cggtacgcag acggtactgg aaatcagccc ggaaatccct gactggctgc gtcaggtggt
     4261 ggatgtgctg tatgtggtcg ccggaatgct tggcgggctg gctggcgcct ggcgtcaggc
     4321 ggcaaagatg gggtcgaaat ttggtacaaa atgtgcggca aaatttattg gtggcgaaat
     4381 ggtcgggatg gccatctctg acacagtcat gggattgttc agcaatccgg tagatgtcac
     4441 cactgggcaa aaaattttgc tgccggagac ggattttacc ctgccaggac gcctgcctgt
     4501 cacctgttcg cgcttttacg ccagccacct ggaaactgaa ggattgctgg gcagaggatg
     4561 gcggctgaac tgggagataa acctgcgtga ggatgaaaca tatatcacct tcatcggtgt
     4621 acagggtcgg gaactgagtt atccgaaaga gatgctgata ccgggccacc agatattcga
     4681 cccggaagag cagttttacc tgagccgtct gcatgacgga tgctatgtgc tgcattacac
     4741 cgactgcagc tattacgtgt ttgatgaatt tgatgaccac ggcgtcgcgc cgttactttt
     4801 tatggagacg ccgtaccggc agcgtattgc tttcgggcgt gagaacggca ggctggtgcg
     4861 ggtggcctcc agcagcggac accatctgtt attgcaccgc acgatgacgc aagcagggga
     4921 acgcctgtcg catattgaac ttttgaaggg cgggcgtccc ggtaacctgg tggaatatcg
     4981 ttacgacgat aacgggcaac tgactggcgt ggtgaaccgg gctggtgtga cggtgcgcca
     5041 gtttgcctat gaaaatggcc tgatgacaga gcatcgcaac gcgacgggct ttacctgtac
     5101 ctaccactgg gaagagattg aaggtttccc gcgcgtggtg gaacacacga ccagcgacgg
     5161 cgaggattac cggttccact atgattttgc tggcgggcag acggtggtaa ccggcaggcc
     5221 ggagcagaag tggcagtggt ggtttgatga agagacgtat gtgactgcgc accggacgcc
     5281 gggcggcgga atgtaccgct tcacgtacaa cgaaaaccat tttccggtag cggtggaact
     5341 ccccggcgaa cgcagggtaa cgctggaata cgacaccctg agccgggtgg tgaaagagac
     5401 ggacccggca ggccgggtga cacagacgca gtggaacggc agttttgctg aaatcacgcg
     5461 ccgggcgctg gatgacgacc acgtatggaa ggcggattac aacgaacacg gccaggtgat
     5521 acgggagacg gacccggaag ggcgcgtcac gcggtacggg tatgatgatc aggggctgcc
     5581 ggagacggtc tgccaccccg gtaaacagca ggaccgttac acctggaacg cgctggggtt
     5641 actgagcagc caccgtcgca tcacgggcag tgtgcagtcc tggcagtata cgcagcgcgg
     5701 gatgctggcg cgccatacgg atgaagagaa acgggagacg cgctggcagt acaccccgga
     5761 agggctggtt gccagtctga gcaacggcaa cggggctcag taccggttca gctacgacgg
     5821 cgacggacgg ctgacgggag aacagcgccc ggacggcctg ataaggatgt ttgcgctgaa
     5881 tgcggacggg tttccggtca tcatcccgac gcagggcaca gagggcgggg tgcgcaatga
     5941 acagcaggag cgtgatgcgc tgggccgcct gctgcgcagt gacacgcagc acagtacacg
     6001 gaccttcagc tacaaccgtc ttgaccagat aacggaagtg acgctcaccc ccacggaaga
     6061 aggggaacgg ctgcaccaca tgcaggcgga cacggtgcgt tttgcgtatg accgcagcgg
     6121 gtggctgacg gcagagcaca gcgtgcacgg cagtatcaaa taccggcgcg atgcgctggg
     6181 caatcccacc gatatcacgt tgccggacgg gcagcatctc agtcatcttt actacggcag
     6241 cgggcatctg ctccagacgg cactggacgg tatcacggtc agtgaatatg agcgtgacag
     6301 cctgcaccgt caggtgatac gcacgcaggg gaaactggcg acgttcagcg gctacaacgc
     6361 cgacaaccgg ttaagctggc agcgcagcct gccaggtggc agccagaatc cacagcaggc
     6421 ggtactcccc cggcgcagaa cgacagcgtg acggcgcggg actatatctg gaacgcagac
     6481 ggtgaagtcg gcggcatcaa tgacaaactg cgcggttgcc tggtgttcag ctatgaccgc
     6541 agcggctggc tgaccagccg cacggggcag atgtatgacc atgaccatta ttattacgac
     6601 aaagcgggca acctgctgac ggatgagtat cagggagcgg tgatggataa ccgcctgccg
     6661 ggttacgggc gtgaccgcta ccgctataac gaatggggcg aactgacaga gcgcagggac
     6721 cagcagctgg agtggaacgc gcagggccag ttaacgcggg tcatcagcag caacagcgag
     6781 acgcggtatc agtacgatgc gctgggcagg cgcatcagca aggcgaccag caatctccat
     6841 accgaccggg gagaacgaag ccggacaacg tttgtgtggg aagggttccg gctgttacag
     6901 gagacaacgt ggcagggaaa gcggacatac ctgtacgatg cggaacagcc gtacacgccg
     6961 gtggctgcca tcacggggcg cggagagagc cagaaaatct ggtattacca cacggacctg
     7021 acgggcacgg tacacgaggt aaccgcgccg gacgggacgc tggtgtgggc gggctaccag
     7081 gcgggatttg gtgaaaaccg gggcgacatc agcaacagcg gggcgtactt tgagcagccg
     7141 cagcggctgc cggggcagta ctttgacgaa gaaacagggc tgcattacaa tctgttcagg
     7201 tattatgcgc cggagtgcgg gaggttcgta agccaggacc cgataggatt aaacggtggg
     7261 ttgaacttat atgcttatgc gcctaatccg ttaggttgga tcgatccgct aggactgaat
     7321 tctttaggaa tagattcaaa taatatattt agaggagatg gtaactataa aggtggcgga
     7381 attggacagc caaagagtgg cacaatatca gcacaggaca ttattgatca tctctaagaa
     7441 aaaggtgatg ggcgtttaac atcttttagt actaaaaaaa catctagttc caactctcgt
     7501 ggtgcagatt ttttctcaaa aaaacatgtc ttcaaagtgg cagtttctga tctcgatgat
     7561 ttagttaaaa ataatcagat cactgtttta acacaagaaa aagtaaaagc aatatttggt
     7621 ttagaatcaa gcaagaaaat tagaaaagaa cttggtaata ttcttcagaa tatggctagg
     7681 aataatgagg tgttagttca tgggcaaatt cttggaaata aagttagccg atgtaattag
     7741 ttagtggaga ggaagttgtg gacgaaatta gaataagaat tgctggtaat acggctgcgc
     7801 cttgtttcca tgtgctaaaa aataagggat ataaagtaac aatggaaact catgcattat
     7861 ctgacgattg tttagattgt gtatatgatt ggtatgcgga aaaaaatgga caatttttta
     7921 gtgcaacgag tccagaagag ttacttggat tagttgcaat gtgggaagtt cgtggtaatg
     7981 actggagagc taatttgaat gagataaata cttatcaaaa agccatagaa gaatccccgt
     8041 tttttgataa aaacggcaat gaaatagagt ctgactaaat tttattgata tagcaacgat
     8101 aataaccgga aatttcttcc agatctttcc ggctttttat tatttaactt tcgttcagca
     8161 cactaaacat cccgtctcgc atccccttaa ctggctgttt aacctgataa agttccctgc
     8221 gcagttcctg tacttcgttg ttatcccaag caggatcaaa catccctccg agatcttcac
     8281 ggtaacgccc gtgccgatct caaatcccgc ttttctcagc catttggcgc tgattttgat
     8341 caccggcgtg tcgctgcggt tgggcagata acccacggta cagcgacgct gtgttttgga
     8401 aatggtgact tctggcgtaa tattgtgttt aagttagggg ctttcgtaat ctagcccagc
     8461 ccccggccct aaacacaacc cccactcacc acaacctaaa ctcatccgca tcccgccatg
     8521 ccggaaactt ttctctatat tcccgcaatg ccaccatcga cagctccgca tcaatacgcg
     8581 ttgccttatg cgcgtcggcg gtagcgataa tctcgccttg cggattaatc acccggctgt
     8641 caccgcgata atggcagccg ttgccatcgc tgccgacgcg attgcatccc gccacatacg
     8701 cctgattctc aatcgcgcgg gccgtcagca atgcctgcca gtgcagagag cgcggagctg
     8761 gccagttggc gacgtacagg gcgaggtcat aatcgttgag attgcgcgac cacactggaa
     8821 aacgtaagtc gtagcacacc agcggcaaaa tacgccagcc gcgccattcc acaatcactc
     8881 gcgcattgcc cgctttataa tgcagatgct catctgccat gcggaacaga tgacgcttat
     8941 cataaaaatg taccgtgccg cccggctcaa ccagcaaaaa gcggttaacc gaaccagact
     9001 ccgtttgtaa tgcaacactg cctgcaatca gcgcattgca ctgctgcgcc ttagctgtca
     9061 tccagttcac tacgtcatct tgtgcaagcg acgaagctgc cgcttccatg gcaaagccgc
     9121 tggtaaacat ctccggtaga acgatcacat cgcgcccggt aataccttcc agttgacgat
     9181 caaaatggcg caggttggca ggaccatcca cccacaccaa cggttgctgc aaaagcgtaa
     9241 tcttcaaact aggcacggtg tacaactcct ttatgcgaag ggttttataa ctttaacacc
     9301 ttatcaggca gttgccttag cgcagaataa attgataaca aatgctgata ttggaaatat
     9361 ctgatttgca aattatcgtg ttatcgctag gctttaggag gttaataaca tgggcaggat
     9421 aagctcggga ggaatgatgt ttaaggcaat aacgacagtc gccgcactgg tcatcgccac
     9481 cagtgcaatg gcgcaggatg atttaaccat tagcagcctt gcaaagggcg aaaccaccaa
     9541 agctgcgttt aatcagatgg tgcaagggca taagctacct gcatgggtga tgaaaggcgg
     9601 tacttatact cccgcacaaa ccgtaacgtt gggagatgag acgtatcagg tgatgagcgc
     9661 gtgcaaaccg catgactgtg gctcgcaacg tatcgctgtg atgtggtccg agaaatctaa
     9721 tcagatgacg gggctgttct cggctattga tgagaaaacg tcgcaagaga aactcacctg
     9781 gctgaatgtg aacgatgcgc tttcgattga tggtaaaacg gtgctgttcg cggcgttgac
     9841 cggcagcctg gaaaaccatc cggatggctt taattttaaa taattagcgg ataaagaaac
     9901 ggagcctttc ggctccgtta ttcaattacg cggcttcaac tttccgcact ttctccggca
     9961 actttaccgg cttcgtcgcc agctcttccg gatcaaagtc atcaacgtta atactgcgca
    10021 gacggctttc ttccgctttc accagaatag cggcttcatc tttatcaatc agccccttcg
    10081 tcagcgcgtt gtgcgccagt tcatccagac gggtaaatgg cagattttta cccagctctt
    10141 tacagatccg ctgatgaatc gggtcggcgg caatcacatc caccaacgcc tcttccagta
    10201 aaccaaccgg gttatgctcg cttggcgtca ggtactgacc gcgaccaata cgtgaacgag
    10261 tggcgttcgg cacttgtaaa atcttcgcca ttttatgatc cagcttgtca gacggtgcca
    10321 gataatgacg tccggtcggg aagatcacca cattcagcag cccggcaacc acgcggttcg
    10381 ggaagttttg cagtaaattg tccattgcct gttctgcctg atacagcgca tcctgtacgc
    10441 cccagtgcac cagcggcaga tcggcttcat tacggccttc gtcgtcataa cgcttcagaa
    10501 cggcagaggc gaggtaaagc tggcttaaaa tatcccccag acgtgccgag atacgctcac
    10561 gacgtttcag gctgccgccc agcagtgcca tcgagacatc agaaagcagg gcgaggttgg
    10621 cgctcaggcg gttcaggtgc tgatagtagc gtttagtggc atcgccggtt ggcgtgctgc
    10681 tggttaaacc gcgcgtcagg cccagccaga agctgcgaac tttgttgcta ccgacgtgac
    10741 cgatatgttt gaacaacagt ttatcgaacg cgttgacgtc attgttcttc gccgcttcca
    10801 tctcttccag cacgtacgga tggcaacgaa tcgctccttg tccgaagatc atcatgctgc
    10861 gggtcagaat gttagcccct tcaacggtga tggcaatcgg tgcgccctgg taagcacgcg
    10921 ccaggaagtt gctttgcccg agcataatgc ctttaccgcc ggtaatatcc atcgcatcaa
    10981 taatcgactg ctgcccgcgg tgggtacagt gatacttaac gatagccgac agcacggcag
    11041 gtttttcgcc gagcataatg ccgtaggtaa tcagcgatgc cgcagcatcc attacataag
    11101 cgttcccggc aatacgcgcc agcggctctt caatcccttc catcttaccg atagagattt
    11161 tgaactgacg gcgaatgtga gaatacgcgc ccgttgccag cgctaccgat ttcacgccgc
    11221 cggttgagtt ggaaggcagg gtgatgccgc ggcctaccga gaggcactcc accagcatcc
    11281 gccagccttg cccggccatt ttcggcccgc cgatgatgta atcgatcggc acgaagacat
    11341 ctttaccgcg cgtcggtccg ttctggaacg gtacgttcag cgggaagtgg cgacgaccaa
    11401 tttccacgcc cggcgtggtg gttgggatca gcgcacaggt aatgcctaaa tcttctgcac
    11461 cgccgagtaa tttttccggg tcggagagtt taaacgccag cccaagcacg gtcgcaatcg
    11521 gtgccagcgt aatgtagcgt ttgttccagg tcagacgcat ccccagcacc tgctggccct
    11581 gccattcgcc catgcagaca atcccggtgt ccggaatcgc gcccgcatcg gaacccgctt
    11641 ccgggctggt cagtgcaaag caggggatct cctgaccacg cgccagacgc ggcagatagt
    11701 gatctttctg ctcgtcagtg ccgtaatgtt gcaacagttc gcccgggcct aatgagtttg
    11761 gcacgccgac ggtaatcgcc aggatcccgc tcacgccaga gagtttttgc agcacacgag
    11821 actgggcata agctgagaac tccagtccgc cgtactcttt tttgataatc atcgcgaaga
    11881 agcgatgctc tttaaggtac gcccacaact ccggtggcag atccgccagc tcatgagtaa
    11941 tctggaaatc attcgccatc cggcaggctt cttctaccgg gccgtcgaga aacgcttgct
    12001 cttcagcggt caggcgcggc tgcggatagt tatgcagctt tttccagtcc ggcttgccct
    12061 ggaacaagtc gccctcccac caggtggtgc ccgcatcaat cgcttctttc tcagtgcgcg
    12121 acatcggcgg catcacctta cggaaaccgc gaaataccgg cgcggaaatc atcgacttac
    12181 gcataggcgc aaagttaaat ggcacgagga taatggtcag aggcaccagt acccacgccg
    12241 accacagacc agcaacgccg agggcggctg tccaggcgag caaaatcaga ctgctgataa
    12301 ataagctcac gcggtgatag aacaacgcgc cgagcaggac aaccgtagcg agaatactca
    12361 aaatcatcat aacgaaaagc cccttacttg taggaggtct gaccacttgt gatgatatgg
    12421 ttgtagtgga tgtaaaaaca tttagcaatg tgtttacaat ataattacaa caaagctcac
    12481 attgttgctg tttttatccg cacttcaggt caaaaagtcc tggtcatagc acctgcccgt
    12541 acttctcgct tttggcggta tccggtacac tgcattttgt ctattacatt tatgctgaag
    12601 gatatcctca tgtaccagga tcttattcgt aacgaactga acgaagcggc ggaaacgctg
    12661 gctaactttt taaaagatga cgccaatatt cacgccattc agcgcgcggc ggtcctgtta
    12721 gcagacagct ttaaagccgg tggcaaagtg ctttcctgcg gcaacggcgg ttcccattgc
    12781 gacgctatgc actttgccga agagttgacc ggtcgctacc gtgaaaaccg tccgggctac
    12841 ccggcgattg ctatttctga cgttagtcat atttcctgcg tcggtaatga tttcggtttc
    12901 aatgatattt tctcccgcta cgttgaagcg gtaggtcgcg aaggcgatgt actgctgggg
    12961 atctccacct ccggtaactc tgcaaacgtg atcaaagcga tcgcagcggc gcgtgagaag
    13021 gggatgaaag tgatcaccct gaccggtaaa gacggcggta aaatggctgg cacggcggat
    13081 atcgaaattc gcgttccgca ctttggttat gccgaccgca ttcaggagat tcacattaaa
    13141 gtgatccata tcctgatcca gttaattgaa aaagagatgg ttaagtaagt ctggcgtagg
    13201 ccggataagg cgtttacgcc gcatccggca cttgtgcgct gatgcctgat gcgatgctga
    13261 cgcgtcttat catgcctaca aatctgtacc cgaaccgtag gccgaataat tcgtttacgc
    13321 cgcatccgac ctgaaaattc ttaaatcaat cttcgccggg gtccatgcgc tcccgctgtt
    13381 gtggaggtta cccatgtgcg aattgctcgg gatgagcgcc aacgtcccta ccgatatctg
    13441 ctttagtttc accggacttg tacagcgtgg tggtggaacc gggccacata aagatggctg
    13501 gggcattacc ttttacgaag gtaaaggctg tcgcacattt aaagatccac aacccagctt
    13561 taattccccc atcgccaaac ttgtccagga ctacccgata aaatcctgtt cggtggtggc
    13621 tcatattcgc caggctaatc ggggcgaggt ggcgctggaa aatactcacc catttacccg
    13681 cgagttatgg gggcgtaact ggacttatgc ccataacgga caactgacgg gctacaaatc
    13741 actggaaacc ggcaacttcc gcccggtcgg tgaaaccgac agcgaaaaag ccttttgctg
    13801 gctgctgcat aaattaacgc agcgttaccc gcgcacgccg ggcaacatgg cggcagtgtt
    13861 taaatatatc gcctcactgg cggatgaact gcgggcgaag ggcgttttca acatgctgct
    13921 ttcggacggg cgctatgtaa tggcgtattg ctcgactaat ttacactgga tcacccgccg
    13981 cgcgccgttt ggcgtggcaa cgttgctgga tcaggatgtg gaaatcgact tcagctcgca
    14041 gaccacaccg aatgatgtgg tcacggtgat tgcgacacag ccgctgacgg gcaatgaaac
    14101 ctggcaaaaa attatgccag gcgaatggcg cttattttgc ctcggggagc gtgtagtttg
    14161 atgccagttg tggctgcacc acttcgtggc ttaacggctt gctgaccacg taacggccat
    14221 tggccacaga aacggtcggt ggcttacggg tttgctcaaa gtagtcgtag cccggcttca
    14281 gctgctccca gaagtcctta aagttggaat atttatggcg cttcatattg gcgtcggtca
    14341 tgcggaatgg gtaaatactc acctgcacgc tcggctgacc aaacaccagc gcaccagtaa
    14401 cgaactggaa gatctcatca ataccctgat tggtcattgc gtagcagccg atggatacac
    14461 aatcgccgtg gatcatcagg tatttccctt cataaccatg cgcacggtca taagcattgg
    14521 ggaatccaat attaatcgct ttgtagtaac ggctgtctgg ttttaactga ttacgctgga
    14581 cgctataaaa cccttccggg cttttgaaat cgccctgacg ctgttttggc cctaagccgc
    14641 cggaatattt acagatttta taactgtcga gcagttgata ttgctcgccc attttgacgt
    14701 aaagatcgag cgtacgttct tccttgaaga tctggatgta gacaggggat cccatcaact
    14761 gctgcttata ctctttgctc actggcgtgg tcgagctact gctgcccagc aaaccggcaa
    14821 acgaaacgca cgggatcaac aacatcgcaa gaattaatgc gattttacgc atactgctta
    14881 ttccttgata aaacggttac acacgccagg acggcaaaat ggatcccaaa tcggaatagt
    14941 ctggatttgg aaggctcaca ttatcaccaa aagagtttta cgcaagcctg tcggcgctgg
    15001 gtttacaaat ttagcacagg ggcagttttc aatagtccga aaagcgcatg tatcatttat
    15061 ttcctaatat tatgaaatct caatagtaat atgcaaataa gcaattaata atcccgttta
    15121 ctcctccggt cttttaaaat tctcaccttc attcagccgg tttatgtctt tcatgtcttc
    15181 gttctggctc ggcagatttt tgcatccagg cgtgttttcg ttatgcgttt tgctcccttt
    15241 actcgccagc gccaccacaa ccgggagcag ataaaacagc atccagaatg gtttcctgcg
    15301 ccgctcaagg cgagtgacag acgctggcag gcgctgacgg aaaacaacca ctttttaagc
    15361 agcgatcacc tgcataacat caccgaagtg gcgattcacc gcctggagca gcagcttggc
    15421 aagccttacg tctggggcgg cacgcggccc gatcaaggct tcgattgtag tgggttagtt
    15481 ttttatgcct acaacaagat ccttgaggcg aaactcccgc gcacggccaa tgagatgtac
    15541 cactaccgtc gggcaacgat tgtggcgaat aacgacctgc gccggggaga tttgctattt
    15601 ttccatatcc acagccgcga gattgccgac catatggggg tgtatctggg cgacggtcag
    15661 tttattgagt cactgcgcac cggggaaacc attcgggtaa gccggttagc cgaacctttc
    15721 tggcaggacc attttttagg cgcgcgcagg attttgacgg aagagatgat tttgtaggcc
    15781 ggataaggcg tttacgccgc atccggcggt tgtgcacgga tgcctgatgc gacgctgacg
    15841 cgtcttatca tgcctacaaa tctgtgcgtt tttgcgagcc gctttcccga tataacacag
    15901 cgtcattgtc ggcctttcct tttcctctta ccgttgagaa aaaggagtcg ctatgtcgga
    15961 atatcgtcgt tattacatca aggggggaac atggtttttc acggtaaatt tacgcaatcg
    16021 tcgaagccat cttctgacca cccagttcca gacgctccgt aacgccatta ttaacgttaa
    16081 gcgagacaga ccttttgaaa ttaacgcctg ggtcgttttg ccggagcata tgcattgcat
    16141 atggacatta cccgaaagcg atgatgattt ttcctctcgc tggcgggaga ttaaaaagca
    16201 gttcacccat gcttgcggat tgaaaaatat ctggcaaccg cgtttttggg agcatgccat
    16261 tcgcaataca aaagattatc gccatcatgt tgattatatt tatataaatc cagagaaaca
    16321 tggctgggta aagcaggtga gtgattggcc attctcaacg ttccatcgcg atgtcgcgcg
    16381 agggttatat cccatcgatt gggcgggaga cataacggat ttaagtgccg gggagcgtat
    16441 cattttataa ctgtgcgctg gtgccggatg cgcttacgct tatccggcct acaaatcgtg
    16501 ctttaacggt aggccggata aggcgttcac gccgcatccg gcagttatgc gcagatgcct
    16561 gatgcgacgc tggcgcgtct tatcatgcct aaaaacttgt gcctgaacgg taggccggat
    16621 aaggcgttta cgccgcatcc ggcaattgtg caccgatgcc ggattcttac atcaacgccc
    16681 ccataatctt caactccagc tcatccggca cttcgttata cgacagcaca tgcagccccg
    16741 gcgcaaacaa ccttgcataa cgcggcagca aagggcgtag ctgcggcggc accagcagca
    16801 ccggatcttt ccccgccgct ttcatctgct ctttcacctg tggcatcgtg ctctggaact
    16861 ggttgagcat attcgggtca accggcacgc tgtcgagcat cactttgccg gcctgttgcg
    16921 cctgattcac tacgttggtc agcagatttt ccagctcatt attcagcgta tacaccgtca
    16981 gctcctgctt gcgaacgaac ggatgagtaa tgctgcgccg cagcgccagg cgcacatcgg
    17041 ccgccagcag aatatgatct ttagtcaccg tgctactggc gaccagcacg gtggcgatgg
    17101 tgacgatatc gcgcagggaa acgccttcgg tcagcagcgc ccggtacact ttcagcaatt
    17161 ggctgtaatt gagcgccgcg cttaaatcct ccgccaggcg cggtgccatc gacgacaaac
    17221 ggttatgcaa ctgcgtgatg tcatcatagt taaacaaatc aggaatatag ctgcgcacaa
    17281 tcttgttcac atgggtggca atcacactgg cgctgtcgat cacctgatac cccatattca
    17341 gagccttcgc tttctgcgca ggctggatcc aggtgaccgg tatcccgtac gccgggtcgt
    17401 tccccagcac gccgtcaatc tcgccgtagg tttcgctgga gggcagcgcc atcagtttat
    17461 ccgccggaat atccgcttca tcagcctcaa tgccgttgat gaaaatagcg tactggctgg
    17521 gcttgaggcg gaagttttcc cgaatgcgga tctccggcag cagcacgccg ttaccgtcgg
    17581 aaatcacctg ccgaacgccg cgaatccgct gggtgagcgg gttgccctgc gctttgtcca
    17641 ccagcgccac cagcttgtag ccgaggctta agctgatggg ctcgatcagc ggaatagttt
    17701 cccaactgac ctgttgttcg ctggtttcag tgatggtgcg ggtcagcgtt tcgaggcttt
    17761 tctcttccgc ctccgccgcc tgcgggcgtt tgctcatccg ccagccggta aagccaagca
    17821 gggcgctgaa cagcaaaaac ggcagatgcg gcatccccgg caccaccgcc agcacgaaca
    17881 tgatcccggt ggcggtgtag agcaccgacg ggcttgccag caactggtgg cgcacgtcat
    17941 gggcgatatc gccgttgtcg ctgacgcggg tgacgataat cgccgccgcg gtggagagca
    18001 gcagggaagg gatctgcgcc accaggccgt cgccgatggt catcagcacg tattgctgga
    18061 aggcggcgtc ggcgctcagg ttgtatttga agatgccgat acagacgccg ccgctcaggt
    18121 tgatcgccag aatcatcatc ccggcgatgg cgtctccgcg tacaaacttc gatgccccgt
    18181 ccatcgcgcc gtagaaatcg gcctcgctgg caacatcttt acgccgggtt tgcgcctgcg
    18241 cctggttgat caatccggcg ttaagatcgg cgtcaatcgc catctgtttg ccgggcatcg
    18301 cgtccagggt aaagcgggca gaaacctcgg aaatacgctc ggcctctttg gtgacgacga
    18361 taaagttgat tgtcaacagc gtcagtaaat ccgagcgtga aagcattatc gccgcgctgc
    18421 acgggcagtc gatttttaac ggtggtgggc tgtcgccgtt aaataaaatc agcccgtctc
    18481 atccgccaaa acctgccacc gtggcggtgc cggaagagac ggagaaaaaa gcgcgtgatg
    18541 tgaatgagaa aacggcgctg ctgaagaaga aatccgccac cgagcttggc gagttggcaa
    18601 ccagtatcaa caccattgcc cgtgatgcgc atatggaagc gaatctggag atggagattg
    18661 ttccccaggg attacgcgtg ctgattaaag acgaccagaa tcgcaatatg ttcgaacgcg
    18721 gcagcgcgca gattatgccg ttctttaaaa ctttgctggt ggagctcgcg ccagtgttcg
    18781 actcgctcta taacaaaatt attattaccg ggcataccga tgcgatggcc tacaaaaaca
    18841 atatctacaa caactggaac ctttcgggcg atcgcgcgct ttcagctcgt cgggtgctgg
    18901 aagaggccgg aatgccggaa gataaagtga tgcaggtaag cgcaatggcg gaccagatgc
    18961 tgctggacgc gaagaatccg caaagcgcgg gcaatcggcg tattgagatt atggtgctga
    19021 ccaaaagtgc gtccgatacg ctgtatcaat actttggtca gcatggggat aaagtggtgc
    19081 agccgctggt gcaaaagctg gataagcagc aggtgctttc gcagcgaatg cgctaaatgc
    19141 tgaatcttta cgcatttctc aaaacctgaa atcactgtat actttaccag tgttgagagg
    19201 tgagcaatgc gtaaaatcat tcatgtggat atggactgct ttttcgcagc ggtggagatg
    19261 cgcgacaatc ccgctctgcg cgatatccct attgctattg gcggcagccg cgaacgtcgg
    19321 ggggtgatca gcaccgccaa ttatcccgcg cgtaaatttg gcgtacgtag cgctatgcct
    19381 actgggatgg cgctcaaatt atgcccacat ctcaccttgc ttccggggcg ctttgacgcc
    19441 tacaaagaag cctcaaatca tatccgtgaa atcttctcgc gctacacctc gcgcattgaa
    19501 ccgttgtcac tggatgaggc ttatctcgat gtcaccgata gcgtccattg ccacggttct
    19561 gcgaccctca tcgcccagga aatccgccag acgatcttca acgagctgca actgacggca
    19621 tctgcgggcg tggcaccagt aaagtttctc gccaaaatcg cctccgacat gaataaaccc
    19681 aacggtcagt ttgtgattac gccggcagaa gttccggcat ttttacaaac cttaccactg
    19741 gaaaaaatcc ccggcgtcgg caaagtctcg gcggcaaaac tggaagcgat ggggctgcgg
    19801 acctgcggtg atgtacaaaa gtgtgatctg gtgattctgc ttaaacgctt tggcaaattt
    19861 ggccgcattt tgtgggagcg tagtcagggg attgacgagc gcgacgttaa cagcgaacgg
    19921 ttacgaaaat ccgtcggcgt ggaacgcacg atggcggaag atattcatca ctggtctgaa
    19981 tgtgaagcga ttatcgagcg gctgtatccg gaacttgaac gccgtctggc aaaggtaaaa
    20041 cctgatttac tgattgctcg ccagggggtg aaattaaagt tcgacgattt tcagcagacc
    20101 acccaggagc acgtctggcc gcggctgaat aaagctgact taatcgccac cgcgcgtaaa
    20161 acctgggatg aacgccgcgg cgggcgcggt gtgcgtctgg tggggctgca tgtgacgttg
    20221 cttgacccgc aaatggaaag acaactggtg ctgggattat gatgtatact attatgtata
    20281 ttctggtgtg cattattatg agggtatcac tgtatgcatc gaattctcgc tgaaaaatcg
    20341 gtcaatatca ctgagttacg taaaaaccca gctaaatact ttattgatca accagttgcg
    20401 gttctttcta ataatcgccc cgcaggatat ctcttaagtg ccagcgcatt cgaagcgtta
    20461 atggacatgc ttgctgaaca agaggagaaa aagcccataa aggcgcgctt tcgtccaagt
    20521 gctgcaagat tagaggaaat tacacgccgc gctaaacaat atcttaatga tatgacggat
    20581 gatgatttca atgactttaa ggaataagga tgcgggtatt caaaacaaaa cttattcgcc
    20641 tgcaacttac agcagaggaa cttgatgcgt taacggcgga ttttatttcc tataagcgtg
    20701 acggtgtttt gccagatata tttggtcgcg atgcactcta cgacgactcg tttacctggc
    20761 cattaatcaa atttgagcga gttgctcata ttcatctggc aaatgtgaat aatccatttc
    20821 cgccacagtt gcgccaattc agcagaacga atgacgaagc gcatttggta tattgtcagg
    20881 gtgcgtttga tgagcaagca tggttgctca ttgccattct gaagcctgaa cctcataaac
    20941 tggctcgaga taacaaccaa atgcataaaa ttggaaaaat ggcagaagcg tttcgcatgc
    21001 gtttttgaat ttatattatg aataacatac aaataagaga ttatcagcct ggcgattttc
    21061 agcaactatg cgctattttc attagagcgg ttacgatgac cgtcagtcag cattattcac
    21121 tacaacaaat tgccgcctgg gcgcagattg acgaatctcg ctggaaggag aaactcgcga
    21181 aatcacaagt gcgggttgca gttattaatg caaaactggt cggctttatt acctgcgttg
    21241 aacactatat cgatatgtta tttgttgacc ctgaatacac ccgccgtggg gttgccagcg
    21301 ctttgttaaa accttttatt aagtctgaat ccgaacttac ggtggacgca agcataaccg
    21361 caaaaccctt ttttgaacgt tatggttttc agacagttaa gcagcagcgc gttgaatgcc
    21421 ggggagcgtg gtttattaat ttttatatgc gttataaacc gcaacattaa atccagcttg
    21481 caatgaaaat aacgcccgcc tagtatgtgc gcgttttctt attccacaaa ctgcaaggag
    21541 gtaaatcatg acaaatcctt tatcaatgac tctttgcaga cctttccagg attaattctt
    21601 tttttcttgc cctggattcg tctgccattt cctgattttt taattgttac tctcaataat
    21661 tcaagtcgca gcacatgcaa cttgaagtat gacgagtcta tttacggaat ggtatatggg
    21721 caattatatt cgtcccttat ccgatgcggt atttaccatc gcatctgatg acctgtggat
    21781 cgagagttta gcgatccaac aattacacac cacggcaaat ttacccaaca tgcagcgcgt
    21841 agtggggatg ccagatttac accccggacg cggctacccg attggcgcag cgttcttctc
    21901 cgcaggccgt ttttacccag cactggtcgg caatgatatc ggctgtggca tggcgctatg
    21961 gcaaacagat attctcgctc gcaaatacaa cgccgataag tttgaaaagc gattatcagc
    22021 tttggatgac gttgctgaag aaagctggct ggaggaaaac ctgccgtcag catttgcaca
    22081 gcatccgtgg cgcagctcgc ttggctctat cggtggtggc aatcatttcg ctgaactgca
    22141 acaagttgat caaatcatca acgctgaact gtttgcactg gcaggtctgg atgcgcagca
    22201 tctgcaactg ctggttcata gcggctcgag ggggctaggg cagtctattt tacagcggca
    22261 tattgcctcg ttttcgcatc atggtttgcc tgaaggcagt gacgacgcgc tggcgtttgc
    22321 gcgttttaat cgccacctga tcgctttgcg cataatgcaa caggttaagg ccaccggaag
    22381 tccggttctg gatgtggcgc ataactttgt tagcgcgtgt cgaatcggtg atcaacaagg
    22441 ggtcttacat cgtaaaggtg ccacgcctga tgactgtggt ctggtggtta ttcccggttc
    22501 acgcggtgat tactccaggc tggttcagcc cgtcaggagt gaggaaacat tgcattcgct
    22561 ggcgcatggg gctgggcgta aatgggggcg caccgagtgt aaagggcgtc tggcagcgaa
    22621 atacacagcg acgcaactct cgcgtactga acttggcagt cgggtaattt gtcgcgataa
    22681 acaactcatc ttcgaagaag cgccacaagc ctataaatcg gctgaaagtg tggtgcaatg
    22741 tcagggcagg ccggattaat tattcctgtc gcgcggctgc gtccggtgct aacgctcaaa
    22801 aacagtggag ggaaaaaagg atgatcttgc tataactctc ctcagcgcag gggccggaag
    22861 aatgttgtct cgcagtgaaa aaggcgctgg acaggctaat taaagaagct gcccgacaag
    22921 acgtcgcggt aacggtgctg gaaacagaaa caggccgcta ctctgacacg ctgcgttcgg
    22981 cgctgatttc tctggatggc gacaacgcat gggcgttaag cgaaagctgg tgtggcacta
    23041 ttcagtggat ttgcccgagc ccgtatcgcc cacatcacgg gcgtaaaaac tggtttctgg
    23101 gcattgggcg ttttaccgct gatgagcagg agcaatccga tgcgatccgg tatgagacgc
    23161 tgcgttcgtc ggggccgggc ggtcaacatg tcaataaaac cgactcggcg gtacgcgcca
    23221 cgcatttggc atccggtatt agcgtgaagg ttcagtcaga gcgcagccag catgctaaca
    23281 aacggctggc gcgattgctg attgcctgga agctggatca gcagcaatag gaaaatagcg
    23341 cggtgctgaa atcacagcgg cgaatgttcc atcaccagat tgaacgtggc aacccgcgac
    23401 gaacgtttac agggatggct tttatcgaag gataatgaag gatgaaactg ccggaaggcg
    23461 attaaacgcc atccggcagc aaatcaaata attacttcgc cggaatttct ttcagcagtt
    23521 cagtcagcag tgtccagtaa tgacctacgc tttcgatgtg aacttgctca tccggagagt
    23581 gtggaccggt gatagttggc ccgatagaaa ccatgtccat ttccggatac ggttttttga
    23641 acagaccaca ttccaggccc gcgtggataa tctggatgtt cggcgtcttg ttgaacaggc
    23701 gctgataggt ttcacgtacc agatgcatca ccggagaatt agcgtccggc tgccagccag
    23761 gatatgcgcc tttcgcttcg gttttcgcgc cagccagttt acccagcgaa tccagcatgc
    23821 tcaccacgta gtctttaccg ctgtcgatta gtgaacggat caggcagtga atttctacgt
    23881 tattgtcagt catggtcacc acaccgacgt tcagggaggt ttcgaccaca ccttttgcca
    23941 cgtcggaatt gcggatcaca ccgttcgggg tggcgttcag cagacgaata aaggtatcgc
    24001 gagatttcgc aatcagggca gctttatcgt tcgctacaga gtccagcaac aaggccagat
    24061 ttttctcttt ctctgccagc tcgtttttca ggatctcctg ataggtattc accagagatt
    24121 tcagtgcgtc gactttatca gctgcgacag caatggtcgc aaaggcttca cgcgggatgg
    24181 cgttacgcag tgtgccgccg ttgaaatcga taaggcgcag gtccagttct tctgcatgac
    24241 ccgccaggaa gcgcaccagc agtttgttgg cattacccag gccaacgtgg atttcaccgc
    24301 cggagtgacc gcctttcaga ccttttaagg ttaacttgaa ggtttcaaaa ccagctggaa
    24361 ccgcttcacg atctaaatgc aggttggagg tgaagtcgat accccccgca caacccatgt
    24421 agatttcacc ttcttcttcg gagtcggtgt taatcagaat atcagcctgc aaccagttgc
    24481 tctgtaagcc gaacgcaccg tccataccgg cttcttcggt catggtcagc agcacttcca
    24541 gcgggccgtg aaccacgttt tcgtcagcca gaaccgccag tgcagaggcc ataccaatgc
    24601 cgttatccgc acccagcgtg gtgccgcgcg ctttaaccca ttcgccatca atataaggct
    24661 ggataggatc tttcgtgaag tcatgcacgg tgtcgttatt tttctgcggc accatatcga
    24721 ggtgggcctg taagacgacc ggtttacgat tttccatacc tgcggtagca ggttgacgaa
    24781 tcaggatatt acctacctga tcgcgttcga catggaaacc tttctctttt gcccaaccaa
    24841 caatgtattc agcgagttgc tcttcatgat aggacgggtg aggaatagaa cagattttgg
    24901 caaaaatatc ccacagcggc tgtggagata attgagacag ttcagacacg ttaagtctcc
    24961 ttgtcgatca cccgcaaaac agtattgcag gtcacagggt tagcagaaaa tgttgtcaac
    25021 acaagacagg cttgcgagat atgtattgag aataccactt tatcccgcgt cagggagagg
    25081 cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta
    25141 taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg
    25201 gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg
    25261 taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg
    25321 tggcggtctg gtaccggggg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac
    25381 cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc
    25441 agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc
    25501 tgttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccgg
//
LOCUS       DAJTSI010000052        24653 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000052 DAJTSI010000000
VERSION     DAJTSI010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.052"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..386)
                     /locus_tag="OO327_001876"
                     /pseudo
     CDS             complement(1..386)
                     /locus_tag="OO327_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            910..2838
                     /gene="thrS"
                     /locus_tag="OO327_001877"
     CDS             910..2838
                     /gene="thrS"
                     /locus_tag="OO327_001877"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR6018431.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2842..3384
                     /gene="infC"
                     /locus_tag="OO327_001878"
     CDS             2842..3384
                     /gene="infC"
                     /locus_tag="OO327_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR6018432.1"
                     /translation="MKGGKRVQTARPNRINGEIRAPEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3481..3678
                     /gene="rpmI"
                     /locus_tag="OO327_001879"
     CDS             3481..3678
                     /gene="rpmI"
                     /locus_tag="OO327_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR6018433.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3731..4087
                     /gene="rplT"
                     /locus_tag="OO327_001880"
     CDS             3731..4087
                     /gene="rplT"
                     /locus_tag="OO327_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR6018434.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4204..4328
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4210..4254
                     /gene="pheM"
                     /locus_tag="OO327_001881"
     CDS             4210..4254
                     /gene="pheM"
                     /locus_tag="OO327_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR6018435.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4537..5520
                     /gene="pheS"
                     /locus_tag="OO327_001882"
     CDS             4537..5520
                     /gene="pheS"
                     /locus_tag="OO327_001882"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR6018436.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5535..7922
                     /gene="pheT"
                     /locus_tag="OO327_001883"
     CDS             5535..7922
                     /gene="pheT"
                     /locus_tag="OO327_001883"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR6018437.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7927..8226
                     /gene="ihfA"
                     /locus_tag="OO327_001884"
     CDS             7927..8226
                     /gene="ihfA"
                     /locus_tag="OO327_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR6018438.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8327..9307
                     /gene="btuC"
                     /locus_tag="OO327_001885"
     CDS             8327..9307
                     /gene="btuC"
                     /locus_tag="OO327_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR6018439.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9370..9921
                     /gene="btuE"
                     /locus_tag="OO327_001886"
     CDS             9370..9921
                     /gene="btuE"
                     /locus_tag="OO327_001886"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR6018440.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9921..10670
                     /gene="btuD"
                     /locus_tag="OO327_001887"
     CDS             9921..10670
                     /gene="btuD"
                     /locus_tag="OO327_001887"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR6018441.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10748..11212
                     /locus_tag="OO327_001888"
     CDS             10748..11212
                     /locus_tag="OO327_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6018442.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11459..12172
                     /gene="ydiV"
                     /locus_tag="OO327_001889"
     CDS             11459..12172
                     /gene="ydiV"
                     /locus_tag="OO327_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCR6018443.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12236..13672
                     /locus_tag="OO327_001890"
     CDS             12236..13672
                     /locus_tag="OO327_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR6018444.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="OO327_001891"
     CDS             complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="OO327_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR6018445.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="OO327_001892"
     CDS             complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="OO327_001892"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR6018446.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="OO327_001893"
     CDS             complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="OO327_001893"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR6018447.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16367..18745
                     /gene="ppsA"
                     /locus_tag="OO327_001894"
     CDS             16367..18745
                     /gene="ppsA"
                     /locus_tag="OO327_001894"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR6018448.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="OO327_001895"
     CDS             complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="OO327_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCR6018449.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20505..20798)
                     /locus_tag="OO327_001896"
     CDS             complement(20505..20798)
                     /locus_tag="OO327_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR6018450.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20795..22084)
                     /locus_tag="OO327_001897"
     CDS             complement(20795..22084)
                     /locus_tag="OO327_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6018451.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22140..23078)
                     /locus_tag="OO327_001898"
     CDS             complement(22140..23078)
                     /locus_tag="OO327_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCR6018452.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(23098..23862)
                     /locus_tag="OO327_001899"
     CDS             complement(23098..23862)
                     /locus_tag="OO327_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCR6018453.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            24181..>24653
                     /locus_tag="OO327_001900"
     CDS             24181..>24653
                     /locus_tag="OO327_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCR6018454.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDALAYTLA"
BASE COUNT         6102 a         5951 c         6484 g         6116 t
ORIGIN      
        1 attaccctgt tcctgttcaa tataatccag tatgcttcgc aaatggttag ctttaatagg
       61 cgtggtttct agtaataagg aaatgatttc ttttttatag tgtaatgtaa tgagtatatt
      121 ttctgcatgg atgggtgaag tggcaaagtt aatggtaaaa agggcagtgg aataacaatt
      181 ttgtgatatt ttcgacagtt tgcggtaaat tcctgagtaa cacagtatga cagcatcgaa
      241 cttaagtcag aggtatcaag tagggtgatg agctggcgcg caaatttatt aataggctct
      301 gatgaaggtg ttggtatgtc gctatcgatg tgcaatctat gaggagactt ataatgagtt
      361 ctgataatga tatcgttttg actcataatc tattcgcctt atatatatct ggaggttttg
      421 aggatgatga ttaattatta tcgccagaac gaaatgtgca cgaaaaattg ttataagaat
      481 tgtgtcactt ttatcattgt gatatgacca cttcgtcatt aatctcatga gcgtttaaaa
      541 tactaccgcc aaatcacgca acgaactttg ctggattcag tttatgctgc tgtaaatccg
      601 ctcgcagtaa acctttcaga cgcaccgtga tgttatcagt ggttctttaa gcgttttgct
      661 ggtgtactca ctacaaacga attgcgaatc gaatcaatgt gaaacggaaa ggtacaatct
      721 cccttttttt agtgttaaca tcgttcaacc gggtggaagg tgagaggtta atgtctttca
      781 tttgtgtacc tgattgcgaa ccaatttagc atttgttggc tagatggttt cgcaatgaac
      841 tgttaataaa caaatttttc tttgtatgtg atctttcgtg tgggtcacca ctgcaaataa
      901 ggatataaaa tgcctgttat aactcttcct gatggcagcc aacgccatta cgaccacgct
      961 gtaagcccca tggatgttgc gctggacatt ggtccaggtc tggcgaaagc ctgtatcgca
     1021 gggcgcgtta atggcgaact ggttgatgct tgcgatctga ttgaaaacga cgcacaactg
     1081 tcgatcatta ccgccaaaga cgaagaaggt ctggagatca ttcgtcactc ctgtgcgcac
     1141 ctgttagggc atgcgattaa acaactttgg ccgcatacca aaatggcaat cggcccggtt
     1201 attgacaacg gtttttatta cgacgttgat cttgaccgca cgttaaccca ggaagatgtc
     1261 gaagcactcg agaagcggat gcatgagctt gctgagaaaa actacgacgt cattaagaag
     1321 aaagtcagct ggcacgaagc gcgtgaaact ttcgccaacc gtggggagag ctacaaagtt
     1381 tccattcttg acgaaaacat cgcccatgat gacaagccag gtctgtactt ccatgaagaa
     1441 tatgtcgata tgtgccgcgg tccgcacgta ccgaacatgc gtttctgcca tcatttcaaa
     1501 ctaatgaaaa cggcaggggc ttactggcgt ggcgacagca acaacaaaat gttgcaacgt
     1561 atttacggta cggcgtgggc agacaaaaaa gcacttaacg cttacctgca gcgcctggaa
     1621 gaagccgcga aacgcgacca ccgtaaaatc ggtaaacagc tcgacctgta ccatatgcag
     1681 gaagaagcgc cgggtatggt attctggcac aacgacggct ggaccatctt ccgtgaactg
     1741 gaagtgtttg ttcgttctaa actgaaagag taccagtatc aggaagttaa aggtccgttc
     1801 atgatggacc gtgtcctgtg ggaaaaaacc ggtcactggg acaactacaa agatgcaatg
     1861 ttcaccacat cttctgagaa ccgtgaatac tgcattaagc cgatgaactg cccgggtcac
     1921 gtacaaattt tcaaccaggg gctgaagtct tatcgcgatc tgccgctgcg tatggccgag
     1981 tttggtagct gccaccgtaa cgagccgtca ggttcgctgc atggcctgat gcgcgtgcgt
     2041 ggatttaccc aggatgacgc gcatatcttc tgtactgaag aacaaattcg cgatgaagtt
     2101 aacggatgta tccgtttagt ctatgatatg tacagcactt ttggcttcga gaagatcgtc
     2161 gtcaaactct ccactcgtcc tgaaaaacgt attggcagcg acgaaatgtg ggatcgtgct
     2221 gaggcggacc tggcggttgc gctggaagaa aacaacatcc cgtttgaata tcaactgggt
     2281 gaaggcgctt tctacggtcc gaaaattgaa tttaccctgt atgactgcct cgatcgtgca
     2341 tggcagtgcg gtacagtaca gctggacttc tccttgccgt ctcgtctgag cgcctcctat
     2401 gtgggcgaag acaacgagcg taaggtaccg gtaatgattc accgcgcaat tctggggtcg
     2461 atggagcgtt tcatcggtat cctgaccgaa gaattcgctg gtttcttccc gacctggctt
     2521 gcgccagttc aggttgttat catgaatatt accgattcac agtctgaata cgttaacgaa
     2581 ttgacgcaaa aactatcaaa tgcgggcatt cgtgttaaag cagacttgag aaatgagaag
     2641 attggcttta aaatccgcga gcacactttg cgtcgcgtcc catatatgct ggtctgtggt
     2701 gataaagagg tggaatcagg caaagttgcc gttcgcaccc gccgtggtaa agacctggga
     2761 agcatggacg taaatgaagt gatcgagaag ctgcaacaag agattcgcag ccgcagtctt
     2821 aaacaattgg aggaataagg tattaaaggc ggaaaacgag ttcaaacggc gcgccctaac
     2881 cgtatcaatg gcgaaattcg cgccccggaa gttcgcttaa caggtctgga aggcgagcag
     2941 cttggtattg tgagtctgag agaagctctg gagaaagcag aagaagccgg agtagactta
     3001 gtcgagatca gccctaacgc cgagccgccg gtttgtcgta taatggatta cggcaaattc
     3061 ctctatgaaa agagcaagtc ttctaaggaa cagaagaaaa agcaaaaagt tatccaggtt
     3121 aaggaaatta aattccgtcc tagtacagat gaaggcgact atcaggtaaa actccgcagc
     3181 ctgattcgct ttctcgaaga gggtgataaa gccaaaatca cgctgcgttt ccgcggtcgt
     3241 gagatggcgc accagcaaat cggtatggaa gtgcttaatc gcgtgaaaga cgatttgcaa
     3301 gaactggcag tagtcgaatc cttcccaacg aagatcgaag gccgccagat gatcatggtg
     3361 ctcgctccta agaagaaaca gtaaggcctt caagtagcac tatctgtgga gcctttgggc
     3421 ttcacaggca ttgttcgcct ttgtttcgtt tattaacaat gcgaagtgga agttattaaa
     3481 atgccaaaaa ttaagaccgt acgcggtgct gctaagcgct tcaaaaaaac cggtaaaggt
     3541 ggttttaagc acaagcacgc taacctgcgt cacattctga ctaaaaaagc gaccaaacgt
     3601 aaacgtcacc tgcgtccgaa agccatggtt tccaaaggcg atctgggcct ggtaatcgcg
     3661 tgcctgccgt acgcataagc cgttaacgtt tttaactttt taattagaat atagatacag
     3721 gagagcacat atggctcgcg taaaacgtgg tgttattgca cgcgcacgtc acaagaaaat
     3781 tttgaaacaa gctaaaggct actacggtgc gcgttctcgc gtataccgcg ttgccttcca
     3841 ggctgttatc aaagctggtc agtatgctta ccgtgaccgt cgtcaacgta agcgtcagtt
     3901 ccgtcaactg tggattgcgc gtatcaacgc agcagcacgt cagaacggta tttcttacag
     3961 caaattcatc aatggcctga aaaaagcctc tgttgaaatc gaccgtaaga tcctggctga
     4021 tatcgcagta ttcgacaaag tagcgttcac cactctggtt gaaaaagcga aagcagctct
     4081 ggcataagcc agttgaaaga gggagctagt ctccctcttt tcgtttcaac gccatcaaaa
     4141 cattgacttt tatcgccgta gccttttcaa taaaggtctt ttgaagagta ccaaaaggta
     4201 acgcaagcaa tgaatgctgc tattttccgc ttcttttttt actttagcac ctgaatccag
     4261 gaggctagcg cgtgagaaga gaaacggaaa acagcgcctg aaagcctccc agtggaggct
     4321 ttttttgtat gcgcgttttg aaaaataaaa agccagtcta tcaggcgtaa ttgtcgcagc
     4381 cagtctggac acggccagtg cggagaaacc ggagcgtact taagtacgtg aggatttcga
     4441 gcactgcccg ggaccaaaat ggcaagtaaa atagcctgat gggataggct ctaagtccaa
     4501 cgaaccagtg tcaccactga cacaatgagg aaaaccatgt cacatctcgc agaactggtt
     4561 gccagtgcga aggcggccat tagccaggcg tcagatgttg ccgcgttaga taatgtgcgc
     4621 gtcgaatatt tgggtaaaaa agggcactta acccttcaga tgacgaccct gcgtgagctg
     4681 ccgccagaag agcgtccggc agctggtgcg gttatcaacg aagcgaaaga gcaggttcag
     4741 caggcgctga atgcgcgtaa agcggaactg gaaagcgctg cactgaatgc gcgtctggcg
     4801 gcggaaacga ttgatgtctc tctgccaggt cgtcgcattg aaaacggcgg tctgcatccg
     4861 gttacccgta ccatcgaccg tatcgaaagt ttcttcggtg agcttggctt taccgtggca
     4921 accgggccgg aaatcgaaga cgattatcat aacttcgatg ctctgaacat tcctggtcac
     4981 cacccggcgc gcgctgacca cgacactttc tggtttgacg ctacccgcct gctgcgtacc
     5041 cagacctctg gcgtacagat ccgcaccatg aaagcccagc agccaccgat tcgtatcatc
     5101 gcgcctggcc gtgtttatcg taacgactac gaccagactc acacgccgat gttccatcag
     5161 atggaaggtc tgattgttga taccaacatc agctttacca acctgaaagg cacgctgcac
     5221 gacttcctgc gtaacttctt tgaggaagat ttgcagattc gcttccgtcc ttcctacttc
     5281 ccgtttaccg agccttctgc agaagttgac gtcatgggta aaaacggtaa atggctggaa
     5341 gtgctgggct gcgggatggt gcatccgaac gtgctgcgta acgttagcat cgacccggaa
     5401 gtttactctg gtttcgcctt cgggatgggg atggagcgtc tgactatgtt gcgttacggc
     5461 gtcaccgacc tgcgttcatt cttcgaaaac gatctgcgtt tcctcaaaca gtttaaataa
     5521 ggcaggaata gattatgaaa ttcagtgaac tgtggttacg cgaatgggtg aacccggcga
     5581 tcgatagcga tacgctggcg aatcaaatca ctatggcggg cctggaagtt gacggtgtag
     5641 aaccggttgc cggtagcttc cacggcgtgg tcgttggtga agtggttgag tgtgcgcagc
     5701 atccgaacgc tgacaaactg cgtgtgacaa aagtgaatgt cggcggcgat cgcctgctgg
     5761 acatcgtctg cggtgcgcca aactgccgtc agggcctgcg tgtggcggta gctaccattg
     5821 gtgctgttct gccgggtgat ttcaaaatta aagcggcgaa actgcgtggc gaaccgtctg
     5881 aagggatgct gtgctccttt tctgagctgg gaatttctga cgatcacagt ggcattatcg
     5941 aactgcctgc ggatgcgccg attggcaccg acatccgcga atacctgaaa ctcgatgata
     6001 acaccatcga aatcagcgtg acaccaaacc gtgccgactg cttaggtatc attggtgttg
     6061 cgcgtgacgt tgccgtgctg aatcagctgc cgctggttga accggaaatc gttccggttg
     6121 gtgcgactat cgacgacacg ttgccgatta cagtcgaagc gccggaagcc tgcccgcgct
     6181 atcttggccg tgtggtaaaa ggcattaacg ttaaagcgcc aactccgctg tggatgaaag
     6241 aaaaactgcg tcgttgcggg atccgttcta tcgatgcagt tgttgacgtc accaactatg
     6301 tactgctcga actgggccag ccgatgcacg ctttcgataa agatcgcatt gaaggcggca
     6361 ttgtggtgcg gatggcaaaa gagggcgaaa cgctggtgct gcttgatggc actgaagcga
     6421 agctgaatgc tgacactctg gtcatcgccg accacaacaa ggcgctggcg atgggcggca
     6481 tctttggtgg cgaacactct ggcgtgaatg acgaaacgca aaacgtcttg ctggaatgcg
     6541 ctttcttcag cccgctgtct atcaccggtc gtgctcgtcg tcatggcctg catactgatg
     6601 cgtctcaccg ttatgagcgt ggcgttgatc cggtactgca gcacaaagcg atggaacgtg
     6661 cgacccgtct gctgatcgac atctgcggtg gtgaggctgg cccggtaatt gatatcacca
     6721 acgaagcaac gctgccgaag cgtgcaacca tcactctacg tcgtagcaaa ctggatcgcc
     6781 tgataggcca tcatattgct gatgagcagg taactgacat tctgcgtcgt ctcggctgcg
     6841 aagtgaccga aggcaaagac gaatggcagg cagttgcgcc gagctggcgt ttcgatatgg
     6901 agattgaaga agatctggtc gaagaagtcg cgcgtgttta cggctacaac aacatcccgg
     6961 atgagccggt acaggcaagc ctgattatgg gtactcaccg tgaagctgac ctgtcgctca
     7021 agcgcgtgaa aacgctgctc aacgacaaag gctatcagga agtgatcacc tacagcttcg
     7081 ttgatccgaa agtgcagcag atgatccatc caggcgttga agccttactg ctggcaagcc
     7141 cgatctctgt tgaaatgtca gcaatgcgtc tttctctgtg gaccggcctg ctggcaaccg
     7201 tggtgtacaa ccagaaccgt cagcagaacc gtgtgcgcat tttcgaaagc ggtctgcgtt
     7261 tcgtaccaga tactcaggca ccgttgggca ttcgtcagga tctgatgtta gccggtgtga
     7321 tttgcggtaa ccgttacgaa gagcactgga acctggcaaa agagaccgtt gatttctatg
     7381 atttgaaagg cgaccttgaa tccgttctcg acctgaccgg taaactgaat gaggttgagt
     7441 tccgtgcaga agcgaatccg gcactgcatc cggggcaatc cgcagcgatt tatctgaaag
     7501 gtgaacgtat tggttttgtt ggagttgttc atcctgaact ggaacgtaaa ctggatctta
     7561 acggtcgcac tctggtgttc gaactggagt ggaacaagct cgcagaccgc gtggtgcctc
     7621 aggcgcgcga gatttctcgc ttcccggcga accgtcgtga catcgcggtg gtggtcgcag
     7681 aaaacgttcc cgcagcggat attttatccg aatgtaagaa agttggcgta aatcaggtag
     7741 ttggcgtaaa cttatttgac gtgtaccgcg gtaagggtgt tgcggagggg tataagagcc
     7801 tcgccataag cctgatcctg caagatacca gccgtacact cgaagaagag gagattgccg
     7861 ctaccgtcgc caaatgtgta gaggcattaa aagagcgatt ccaggcatca ttgagggatt
     7921 gaacctatgg cgcttacaaa agctgaaatg tcagaatatc tgtttgataa gcttgggctt
     7981 agcaagcggg atgccaaaga actggttgaa ctgtttttcg aagagatccg tcgcgctctg
     8041 gaaaacggcg aacaggtgaa actctctggt tttggtaact tcgatctgcg tgataagaat
     8101 caacgcccgg gacgtaaccc gaaaacgggc gaggatattc ccattacagc acggcgcgtg
     8161 gtgaccttca gacccgggca gaagttaaaa agccgggtcg aaaacgcttc gcccaaagac
     8221 gagtaatctg atctaactaa aaaggccgct atgcggcctt ttttcttttc actgtcgaag
     8281 agtcaccgta aaatcaacgc catgacactt cagcagaacg gataccatgc tgacacttgc
     8341 ccgccaacaa cagcgacaaa atattcgctg gttattatgc ctgtcagttt tgatgctgct
     8401 ggcgcttctc ttaagccttt gcgccggtga acaatggatt tcgccaggtg actggtttac
     8461 tcctcgtggc gaactgttcg tctggcaaat tcgcctgcca cgtacgctgg ctgtattgct
     8521 ggttggtgcg gcgctggcta tatccggcgc tgtaatgcag gcgttgtttg aaaatcctct
     8581 ggcagaacct ggactacttg gcgtctctaa cggcgcaggc gtggggctta tcgccgcggt
     8641 attgcttggg caagggcaac tccccaactg ggcgctaggg ctgtgtgcga ttgctggcgc
     8701 gcttatcatc actttaatac tcttacgttt cgcccgtcgt catctttcga ccagtcggtt
     8761 attgctggct ggcgttgcat tagggattat ctgcagcgca ctaatgacgt gggctatcta
     8821 cttttccacc tctgttgatt tacgtcagct gatgtactgg atgatgggcg gttttggcgg
     8881 cgtagactgg cggcaaagct ggctgatgct ggcattgatc cccgtgttgt tgtggatctg
     8941 ttgtcagtcc aggccgatga atatgttagc gcttggcgag atctcggcgc ggcaactggg
     9001 tttacccatg tggttctggc gcaatgtgct ggtggcagcg accggctgga tggttggcgt
     9061 cagtgtggcg ctggcgggtg ctatcggctt tattggtctg gtgatcccac atattcttcg
     9121 gttgtgtggt ttaaccgatc atcgcgtatt acttcccggc tgcgcgctgg caggggcgag
     9181 cgcattgctg ctggccgata ttgtagcgcg cctggcatta gctgccgcag agctgcctat
     9241 tggcgtggtc accgcaacgt tgggtgcgcc ggtgtttatc tggttattgt taaaagcagg
     9301 acgttagccg caaaaagacg gtctatgatt aaaagctaag atttttactg accacaccta
     9361 ggagaaacga tgcaagattc cattctgacg accgtagtga aagatatcga cggtgaagtg
     9421 accacgctgg agaagtacgc cggtaatgtg ctgttaattg tcaatgtcgc ctcaaagtgt
     9481 ggcttaacgc cgcaatatga gcagttggag aatattcaaa aagcctgggc cgatcgaggt
     9541 tttgtggtgc tgggattccc gtgcaaccag tttctggaac aagaaccggg cagcgatgaa
     9601 gagattaaaa cttactgtac caccacatgg ggggtgacgt tcccgatgtt cagtaagatt
     9661 gaagttaatg gcgaaggacg ccatccgctg tatcaaaaat tgattgccgc agcgccgacc
     9721 acagtcgcgc cggaagagag cggattctat gcccgtatgg tcagcaaagg ccgtgcaccg
     9781 ctgtacccgg atgatatttt atggaatttt gaaaaattcc tggttggcag ggacggaaaa
     9841 gtcatccagc gtttttcccc ggatatgacg ccggaagatc ccattgtgat ggaaagcatt
     9901 aaactggcgt tggcaaaata atgtctattg tgatgcagtt acaggatgtt gcggaatcta
     9961 cccgcctggg gccgctttca ggcgaggttc gggctgggga aatcctgcac ctggtggggc
    10021 cgaatggcgc gggtaagagt accttactgg cgcgaatggc cggaatgacc agcggtaagg
    10081 gaagcattca gttcgcgggg caaccactgg aagcatggtc cgcaacaaaa ctcgcgctgc
    10141 atcgcgccta tctttcacaa cagcagacgc cgccgtttgc aatgccggtc tggcactacc
    10201 tgacactgca tcagcacgat aaaacgcgta ccgaactact gaatgatgtc gcaggggcgc
    10261 tggctcttga tgacaaactc ggtcgtagca ccaatcaact ttccggcggt gaatggcaac
    10321 gcgtacgtct tgctgcggtg gtgttgcaaa tcacaccaca agccaatccc gcaggccaat
    10381 tgctgcttct tgatgagccg atgaacagtc ttgatgttgc gcaacaaagt gcgttagaca
    10441 aaattctgag cgcgctgtgt cagcaaggac tggcgattgt gatgagcagt cacgatctca
    10501 accacacgtt gcgtcatgcg catcgggcgt ggttgctaaa aggtggaaaa atgctggcca
    10561 gtggacgcag agaagaggtg ctcacgccgc caaatctggc gcaggcctat gggatgaatt
    10621 ttcgccgtct ggatatcgaa ggtcacagaa tgctgatttc gaccatctga tttcattagt
    10681 atcccgaaaa tcgggtaatt acgacgctaa attaatgcaa gaataaaaaa cagaggaacc
    10741 gtacggaatg cgtttctgcc ttattttgat cacagcactg tttctggccg ggtgtagcca
    10801 ccataaagca ccgccgccaa atgccagact ttctgattcg attaccgtta ttgccggttt
    10861 gaacgaccag ctacaaagct ggcatggcac gccgtatcgt tatggtggca tgacgcggcg
    10921 cggtgtggac tgttcgggat ttgtggttgt gacgatgcgc gatcgtttcg atttgcatct
    10981 gccccgtgaa accaaacaac aagcctctat tggcacgcaa attgataaag acgagttgct
    11041 gcctggtgac ctggtctttt tcaaaacggg ttccggacaa aatggtttgc atgtaggtat
    11101 ttacgatacc aacaaccaat ttatccacgc ctctaccagc aagggagtga tgcgttcctc
    11161 acttgataat gtctattggc agaaaaattt ctggcaggcg agacgaatct agtatgtcga
    11221 aaaattgacg gcgatgatac atcgccgtca atttactcct gaatacagta gtattttgca
    11281 gacaaaaatt ccgtaaatgg attattcgtt tataatcagg ataatctaaa aaaaggcccc
    11341 ctgaaaaagt ggttaacatg gaacaaatga aagttactcc ttctgtaatt gttatgtctt
    11401 atggcctcat cccttttagc cggatactga aaaacatcct tcgagaggga cggttaccat
    11461 gaagattttt ttggagaatc tttatcattc tgattgctat ttcctgccga taagagataa
    11521 ccagcaactc cttgtcggcg ttgagttaat tacccatttc tccagtgaag atggcacggt
    11581 tcgtatcccg acgagtcggg ttatagcgca acttaccgag gagcaacatt ggcaattatt
    11641 ttcagagcag ttggaattac taaaatcatg ccaacacttt tttattcaac ataaactctt
    11701 tgcctggctt aatttaacgc cacaagtggc tacattatta ttagacaggg ataattacgc
    11761 aggcgaacta ttaaaatatc cgtttattga gctgttgatt aatgagaatt acccgcatct
    11821 caatgaagga aaagataatc gcgatctttt gtccttatcg caaatgtatc cccttgtgtt
    11881 aggcaatttg ggagcaggta atagcacaat gaaagcggtt tttgatggtc ttttcacccg
    11941 cgtaatgctg gagaaaggtt ttattcagca acaaatcacg catcgctcct tcgaaccgtt
    12001 tattcgtgcg atccaggcgc aaatttcccc ttgttgcaat tgcatcatcg cggggggcat
    12061 tgatacgcca gaaatattgg cacagattac ccccttcgat ttccatgctc tccagggatg
    12121 cctgtggcct gccgttccta taaatcagat aacgacgttg gttcagcgat aaccctcctg
    12181 tttgctggtg tttaagacga gagtaaccgt ctacactatc aaacagatag gatctatgac
    12241 cctgtctttt attacccgct ggcgcgatga attgccagaa acctatacag cactttcccc
    12301 tacgccttta aataatgccc ggctgatttg gcataatacc gaactcgcta acacgctgag
    12361 tattccatcg tcgctgttta aaaatgccgc aggtgtctgg ggcggcgaaa ccctactgcc
    12421 tggtatgtca ccactggcac aggtttacag tggtcatcag ttcgtcgtct gggcgggcca
    12481 actgggtgat gggcgcggca ttttacttgg cgaacaactg cttgctgatg gcactacaat
    12541 ggactggcat ctgaaaggtg ctggcctgac gccttattcg cgaatgggtg atggacgggc
    12601 ggttttacgt tcgacgatac gagaaagtct cgccagtgag gcgatgcatt atctgggcat
    12661 tccgacgacc cgcgcgttaa gtatcgtcac cagcgattcg ccagtgtatc gggaaacggt
    12721 ggagccaggc gcgatgctga tgcgtgtggc accaagtcat ctgcgctttg gtcatttcga
    12781 acatttttac tatcgccgcg agccggaaaa ggttcgtcag ttggctgact ttgccattcg
    12841 tcattactgg tcacatcttg aagacgatga ggacaaatac cgtctctggt ttaacgatgt
    12901 tgtcgcacgt accgcgtcgt taattgccca atggcagacg gtcggctttg cacatggggt
    12961 gatgaatacc gacaacatgt cgctgctggg actgacgctt gattacgggc cgtttggttt
    13021 tcttgatgat tacgaacccg gttttatttg taatcactcg gatcatcaag ggcgttatag
    13081 ctttgataat caacctgctg tcgcgttgtg gaatttacag cgtctggcgc agacattgtc
    13141 accatttgtt gccgtagatg ccctgaatga ggccctggac agctatcagc aggttttgtt
    13201 gacgcattat ggacaacgga tgcggcagaa actgggcttc atgacggagc aaaaagagga
    13261 taacgcgcta ctgaatgaat tattcagtct gatggcgcga gagcgcagcg attatacccg
    13321 cacattccgc atgctgagtc tgaccgagca gcacagcgcg gcgtcaccgc tacgtgagga
    13381 gtttattgat cgtgcggcat ttgatgactg gtttgcccgt tatcgggggc gtttgcaaca
    13441 agacgaggtt agcgatagtg agcgtcagca actgatgcaa agcgttaacc cagctctggt
    13501 attgcgcaac tggttggcgc aacgggcgat tgaagcggca gaaaagggtg atatgatgga
    13561 attgcaccgc ctgcatgagg cgttgcgaaa tcctttcagc gacagagatg atgactatgt
    13621 cagtcgtcca cctgactggg gtaaacggct ggaagtcagc tgctcgagtt aaaggctact
    13681 tagttaacag cagcttgcca gcctgagttt tacggagcag atattcttgc ccgtcatgat
    13741 cgataatcag tttgccatcc ggacctaaca gtgtctggct ggtaattcgc cggatgggct
    13801 gcggggaagc ggtcttgtga ttggcatccg tttcaggcgt gagttttctg ctatccgtat
    13861 aacgcataaa aattaataat aattgatata acaatgattc ctattatcaa tattatggca
    13921 gaccagagca agcagtaatg tgttgagaag acaacaagtt gcgcagaaat gcccatccca
    13981 tcaggaatgg gcacgcattc agaagcgggt atctaccgca aaggcgagtt tttcgaccag
    14041 gcgttcggta tcttcccagc ccagacacgg gtcggtaatg gattgaccgt aagtgagcgg
    14101 ctgaccgccg acgatttttt gcgttccttc gcgcaggaaa ctttccgcca taattccagc
    14161 aatctccgta gagccattgc ggatttgctg acaaatatcc tcacaaactt ctaactggcg
    14221 acggtgctgc ttctggcagt taccgtggct gaaatccacc accagatgtt caggtaaatc
    14281 aaactcgtgc agcgtatcgc aggctgcggc gatatcatcg gcatgataat tcggtttttt
    14341 gccgccacgc ataataatgt ggccatacgg gttgccgctg gtctgataga tggtcatctg
    14401 accatttttg tctggcgaga ggaacatatg gctggcgcgg gctgcgcgga tagcatccac
    14461 ggcaatccgc gtgttgccat cggtaccatt tttaaaacct accggacagg agagtgctga
    14521 agccatttcg cggtggatct gactttcggt agtacgagcg ccaatcgcgc cccaactgat
    14581 taaatcagca ataaactgac cggttaccat atcgaggaac tcagtcgcgg tagggacacc
    14641 cagctcattt acctgtaaaa gtaatttgcg cgccagctcc agaccgtgat ttacccgata
    14701 gctgccgttt aaatccggat cggagattag ccctttccag ccgacaacag ttcgtggttt
    14761 ttcaaaatag gtgcgcatta cgatttccag ccgtgactgg tactggttgc gcagcgactg
    14821 cagacgggtg gcgtactcca ttgcagcggt gagatcgtgg atcgagcagg ggccaataat
    14881 gaccaacagt cgcttatctt caccattcag tattttttca attctgcggc gggagtcggt
    14941 gacatgggtg gcgacgccag gcgttacggg ataccgtagc gcgagttcgg cgggcgttac
    15001 caggctctca atacgcgcag tacggagttc gtcagttctg ttcattacaa gtctcagaaa
    15061 tttgttgctt ctacggcagg actaccggaa agtgatgcgc atcacgataa accaatcgca
    15121 gctgatttca agtgcgaggt gtgtcacacc tcgccgggtg gttagcatga taacaaaaaa
    15181 acaaaccaat gcactagttc tctagtacat tcggcgacta aggcccatga tatcgaggat
    15241 cttggtggca atctcttcta ccgaataatt ggtactgtta atccacggga tctgattttt
    15301 acggtacaag gcttccactt ccgcgacttc catcctgcac tgacgaagcg aggcatagcg
    15361 actgttctcc cgacgttcct cgcgaatcgc cgccagacgt tccgggtcga tagtcaggcc
    15421 gaacaattta tgctgaagcg gtttgagcga cgcgggtagc accagattat ccatatcgtc
    15481 ggcaataaag gggtagtttg ccgcgcggat accaaattgc attgccagat acagactggt
    15541 gggggtttta ccacagcgag aaacaccgag caggatcacc tgagcctgat ccagattgcg
    15601 caacgaaatg ccgtcatcgt gggcgagggt gtaatcaatc gccgcaatgc gcgcatcata
    15661 tttattgaga ttattagggt taaggccatg ggtacgatga gcaatcggcg ttggatccag
    15721 tttcatctct tgttgtagcg gggcaaccag cgcctgaacg atatcctggc aaaagccttc
    15781 actttgcaag atgatggcgc gaatctccgg caacacgatg gagtagaaga ccagcgggcg
    15841 cacgcctgtc tggtgataaa ttgcgtcaat ctgatccttc actgcccgtg cacggctctc
    15901 attttcgaca aacggcagcg tgatgctgct gatagtgacg ggaaattgtg acattactgc
    15961 gtgtcctaat acctccgcag ttattgccgt accatcagaa atataaaaaa cgtggcgatc
    16021 aacagcatta tccattttgt tcttcccgtg aagcagacat aattctatga aagcataaat
    16081 taaaagcgca cagaagcgta ggacgtaatg tctggtttat aaaatgaacc ttcattttat
    16141 tttttataaa aacagcattt catttttatg gtttcgttta taccgatggt ttatgtggaa
    16201 attgtcgaat agagcaggtt tgcgcaacgc tgggatcagt cttaaaaagt aaaaaaatat
    16261 atttgcttga acgattcacc gtttttttca tccggttaaa tatgcaaaga taaatgcgca
    16321 gaaatgtgtt tctcaaaccg ttcatttatc acaaaaggat tgttcgatgt ccaacaatgg
    16381 ctcgtcaccg ctggtgcttt ggtataacca actcggcatg aatgatgtag acagggttgg
    16441 gggcaaaaat gcctccctgg gtgaaatgat tactaatctt tccggaatgg gtgtttccgt
    16501 tccgaatggt ttcgccacaa ccgccgacgc gtttaaccag tttctggacc aaagcggcgt
    16561 aaaccagcgc atttatgaac tgctggataa aacggatatt gacgatgtta ctcagcttgc
    16621 gaaagcgggc gcgcaaatcc gccagtggat tatcgacact cccttccagc ctgagctgga
    16681 aaacgccata cgcgaagcct atgcacagct ttccgccgat gacgaaaacg cctcttttgc
    16741 ggtacgctcc tccgccaccg cagaagacat gccggacgct tcctttgccg gtcagcagga
    16801 aaccttcctc aacgttcagg gttttgacgc cgttctcgtg gcagtgaaac atgtatttgc
    16861 ttctctgttt aacgatcgcg ccatctctta tcgtgtgcac cagggttacg atcaccgtgg
    16921 cgtggcgctc tccgccggtg ttcagcggat ggtgcgctcc gacctcgcat cttctggcgt
    16981 gatgttctcc attgataccg aatccggctt tgaccaggtg gtgtttatca cttccgcatg
    17041 gggccttggg gaaatggtcg tgcagggtgc ggttaacccg gatgagtttt acgtgcataa
    17101 accgacactg gcggcgaatc gcccggctat cgtgcgccgc accatggggt cgaaaaaaat
    17161 ccgcatggtt tacgcgccga cccaggagca cggcaagcag gttaaaatcg aagacgtacc
    17221 gcaggaacag cgtgacatct tctcgctgac caacgaagaa gtgcaggaac tggcaaaaca
    17281 ggccgtacaa attgagaaac actacggtcg cccaatggat attgagtggg cgaaagatgg
    17341 ccacaccggc aaactgttca ttgtgcaggc gcgtccggaa accgtgcgct cacgcggtca
    17401 ggtcatggag cgttatacgc tgcattcaca gggtaagatt atcgccgaag gccgtgctat
    17461 cggtcatcgc atcggcgcgg gtccggtgaa agtcatccat gacatcagcg aaatgaaccg
    17521 catcgaacct ggcgacgtgc tggttactga catgaccgac ccggactggg aaccgatcat
    17581 gaagaaagca tctgccatcg tcaccaaccg tggcggtcgt acctgtcacg cggcgatcat
    17641 cgctcgtgaa ctgggcattc cggcggtagt gggctgtgga gatgcaacag aacggatgaa
    17701 agacggtgag aacgtcactg tttcttgtgc cgaaggtgat accggttacg tctatgcgga
    17761 gttgctggaa tttagcgtga aaagctccag cgtagaaacg atgccggatc tgccgttgaa
    17821 agtgatgatg aacgtcggta acccggaccg tgctttcgac ttcgcctgcc tgccgagcga
    17881 aggcgtgggc cttgcgcgtc tggaatttat catcaaccgt atgattggcg tccacccacg
    17941 cgcactgctt gagtttgacg atcaggaacc gcagttgcaa aacgaaatcc gcgagatgat
    18001 gaaaggtttt gattctccgc gtgaatttta cgttggtcgt ctgactgaag ggatcgcgac
    18061 gctgggtgcc gcgttttatc cgaagcgcgt cattgtccgt ctctctgatt ttaaatcgaa
    18121 cgaatatgcc aacctggtcg gtggtgagcg ttacgagcca gatgaagaga acccgatgct
    18181 cggcttccgt ggtgcgggcc gctatgtttc cgacagcttc cgcgactgct tcgcactgga
    18241 gtgtgaagca gtgaaacgtg tgcgcaacga catggggctg accaacgttg agatcatgat
    18301 cccgttcgtg cgtaccgtag atcaggcgaa agcggtggtt gaagaactgg cgcgtcaggg
    18361 gctgaaacgt ggcgagaacg ggctgaaaat catcatgatg tgtgaaatcc cgtccaacgc
    18421 cttgctggcc gagcagttcc tcgaatattt cgacggcttc tcaattggct caaacgatat
    18481 gacgcagctg gcgctcggtc tggaccgtga ctccggcgtg gtgtctgaat tgttcgatga
    18541 gcgcaacgat gcggtgaaag cactgctgtc gatggctatc cgtgccgcga agaaacaggg
    18601 caaatatgtc gggatttgcg gtcagggtcc gtccgaccac gaagactttg ccgcatggtt
    18661 gatggaagag gggatcgata gcctgtctct gaacccggac accgtggtgc aaacctggtt
    18721 aagcctggct gaactgaaga aataaaataa atccccggcg gcgttcagtc gccggggtta
    18781 tgtgatcccc gaagatgaaa cttattcaat ctcttcacag acatcctgcg ttaatcgccg
    18841 tataatatct tttcttaaca aaaacttttg tattttatct gaggcagtgc gcggtagttt
    18901 ttcgattacc acgatatgtt caggatattt atattttgcg acccgtttac ggctaaaaaa
    18961 agccgccatc tcttccagcg ataatgaatg atgcggcgct ttcagcacga cataagcgca
    19021 tgatcgttca cctaaacgtt catcgggcat tgcaaccaca caggcatcgt gaattttagg
    19081 atgctgcaat aaaatatctt ccacttcacg gctgctaata ttttcgccgc cgcggacaat
    19141 aatatctttt ttgcgtccgg taatttttat atagccagcc tcatccatac ggcagagatc
    19201 gccgctgtaa taccagcctt cttcatccag cgcacgggcg gttaattcag gttcatcaaa
    19261 ataccccata aacacattgg ggccacgcga tgcttcttca ccttcgcaac ctggcggtaa
    19321 ggtcttgcgt gcgtcatcga ccactttaat ctctacacct gcggcagcgt aaccatcggt
    19381 atgcataaag cgcgacaaag aattatcgag attcaccacc gcatgcggcg aactttctgt
    19441 ggaaccataa acacttaata atttaatgct gcgctgctgg cattcgcgcg ccactttttt
    19501 ggggattgtg gtaccgccgc aaagaaagaa gcgcagcgct gaaaggtctg cgggttgttt
    19561 ctctagtaaa ttcaaaagat catagacaaa cggcgttgcg cctagcatac aggtgcaacg
    19621 ctgctgctca agcagcgcga gacacgcatc aggagtgaaa atatctaaca acacgctgcg
    19681 agcgccaatt aagaatggtg ccgttacgcc atgcagaaag cccgttgcgt gaccaagtgg
    19741 cgcaggcatc ataaagacat ccagccaggt cagattcagc cgcgcgcaat aagcccgctc
    19801 actggcgaga atattgttat gcgttagcat cacgcccttt ggcagaccct cggttccgga
    19861 ggtaaacagc accgcagcta attcatcgcc gtgggtcgtt atcgccgtgg tcagtgggat
    19921 attgtcggcg ataatctgac ttaatgagag ggaagaggtg gcgggagcca gtttgtccac
    19981 gccgacaatt tgttgtagtt gtggaagctg attttgcagc ggcaggatta aatctaccgg
    20041 acgcgtttgt ttaaacaacg tcggtgcaaa gaacattttt gcctgacact tattgagcac
    20101 ccacaccagt tctgcttccc gccaggaagg caacagcggc acggaaaccg caccgatttt
    20161 caggcaggca agatagataa cggtaaattc acaccagcca ggcagttgaa atgcgatgcg
    20221 atcgcctgat tcaataccct tcgctaacat ccagtttgcc agacagctcg cggcgtgatc
    20281 gagcgcgcta taggtgtacg atgcaccatg attatcgacc acggcaattt tgtctggcat
    20341 cgcacgagcg gtctgctgcc agtaatcggc cagtgaagca tcgccccata gcccttgctg
    20401 acgatacgcc gcacgacgtt gttcgttaaa cgttaatgtc actttcattt tggactctcg
    20461 aaacagaaca tcaggcccga gttgcgggcc tgtgggatgc atgattagcc gtagcgaaaa
    20521 tcaataccga aggtgcctgc gggatattgc cactgttcga gaatagtgtt accgcacagc
    20581 acccgacagg tgccgcactc cagacaaccg gcgggatcaa aatgaatgtt tcctacgtca
    20641 tcctgcttat aaagtccggc agggcaggct ttcattaatt tacggaattc attgatatcg
    20701 ggattttccg tcagaatgat atgcgggtgg ccctcatcaa catggaattt attgacgcct
    20761 aatttgatgt cgacgttaac cgtagcgttc tggctcatag cgcggttgct cccttaatgc
    20821 catctttcag caagttgatc agcccaattt tcttcgcgtg tcccatgatc attttgcgta
    20881 ccggctggtt tggtttgccg tcaatggtga acatctcgtt catgatgtcg gctaccattc
    20941 gtgggtattg gctaaacagg cgcgggtttt ccatcagcgc cgggatcttg cgaaaatgct
    21001 gcatatcacg catgacgcag ctttgttcca gctcacgttt gtattgcgcc agactgttgg
    21061 cggagaaatc cgcgcgttct ttggcggcga tcaccgttgt ggcggcagcc tgagccgatg
    21121 caatggctaa atccatgccg cggaccgtaa aacccaaatt caggcagaag cctgcggcgt
    21181 caccaacgat catcacgccc tcgttaacca gttgcggcac cattgccaga ccgccttccg
    21241 gcaccatatg cgcggaatat tcaagcagtt tgccgccgct aatcagcggg cgaatggcgg
    21301 ggtgttgttt aaaatcttcc agcatttgcg gcacgctttt ttgcgcatgg gcgatatcac
    21361 ccaatccaca aaccagcccc aaggatatgg aatccttgtt ggtatagaga aatcccccgc
    21421 ccatcaggcc gtcagaaggg gaaccggcaa acagccaggc ggcaccttcc tcgcccgtaa
    21481 tattaaagcg atcgttgatc tgttctggtg tgaggccaat aacctcttta acaccaacag
    21541 cgtaatgatg cggatcggaa gcgggaacca ttcccagcga gcggccaagc atcgagttaa
    21601 cgccatcagc cagaatcacc acattcgctt cgagaatatc atccccagcc tgcacgccag
    21661 tgaccttgtt tccttcacga accagcgcgt cgacgcgaac gcctgggata aactgcgcgc
    21721 ccgcctgctc ggcttgttcc atcaaccacg ggtccagacg attacgcaat acggtataag
    21781 atgcgtgttg aggaacatct ggttgctcgc ggtgaaaatc gagggtaacg gcgctttctt
    21841 cggttaagaa ggagattttc tcgcgtgtga ccttgcgttc taccggcgct gatactgcaa
    21901 agcctggaat gattgcttca agtgtgtggg cataaagacg cccgccggtc atgtttttac
    21961 atccggcact gtcgccgcgt tctatcacca ggacatccag cccggctcgt gccatgacca
    22021 gtgcggcaac gctaccagca acgcccgcac cgaccacaat ggcatcaaat ttgtcatccg
    22081 acatgcgtta tgctcccttc ctgctgcgtt tacagcggga gatgtggttg aaaggggaat
    22141 tagcggctca actggctaat cagggcaggg acgactttgt agatatcgcc caccagaccg
    22201 tagtcggcat agttgaagat tggcgcattt ttatctttat tgatggcgac aatcactttt
    22261 acgccgttgc cgccaaccat atgttggatc tgcccggaga tccccagcgt caggtagaga
    22321 tcggatttca gcaacacgcc ggagacaccg atataacgtt cacgctccat ccagttctcg
    22381 ccttcggcaa ttggacgtga acagccgact tcagcattca gcaccgccgc cagttcgtgg
    22441 accattttca ggtcatcctg cgccgccaga ccacgaccga cgccaaccac acgttttgct
    22501 ttgctcaggt ccacgctgct tgcggctttc gcacggcgtt cgcgacagag aatttcatga
    22561 cgcggagcaa cataaggtac cgtttctgtc gggcactgat gagaggtatc actggtgcac
    22621 ggttcctgaa caccgggtgc aagggtaatg atcgccagcg ggctgttgat cttttcctga
    22681 gcgaacgcta acccgccata catccggtgt tcggcgcaaa tgtgaccatc gacaatatcc
    22741 accgccgtgg catcgttcac cagcgccgca ttcagttgca cacttaaccg tgctgccaga
    22801 gctttaccac gtttcgtcgc ggccaacagc aacatagcgg ggtgtttatc tttcagcagg
    22861 gcagcaatgc tttcggcgta attttcagtg cgttgcagcg cgtcgttttg ctcaagaaca
    22921 taaatacatt ttggaccata aggcataact gcctgcgcct ggtcggtatt ttgtacaatg
    22981 gcatacacct gttggcccca ttgctgagcg ccgccaaaca gttcagcata acgttcagga
    23041 ttatcgctaa agacccagac gctgtttaat tgactcatcg tttcttctcc gtaggcttca
    23101 gttcagggct ttcttcagat gttccgccag ctcggcaatg gcctccggcg aatcgttatc
    23161 gagaatgatg tgcttacgtt ctgtttgcgg cggtacgcga atgccgacca gttcagcaag
    23221 tggcgcgctc tggctccagt caatatcact tgcctgccac tgatttaccg gttttttacc
    23281 cgcgccgaga atggctttca tcgaaggaat gcgtggcacg ttaatatcgg aggtgacgca
    23341 gagcacggct ggaacagaga gttcaataac ttcaacatca tcttcaagcg tgcgttcaat
    23401 caccagtgta ttgccctgac gctgaatagc actcactgca ttaatcaccg gaagttgcag
    23461 aatttctccg accagcaagc caacctgctg ggcataaagg tcgccggaac cttcaccaaa
    23521 gatcagtaaa tcgaagccga tcttttcaat tgctgccgcc agcgcttttg cggtatcgag
    23581 aggcagtgca tgttcaagtt gcgcatcctg caccaaatac aggctgtgcg gcccgcggga
    23641 tagcacgtct ttgcgcactt tcgagttctg caacaatgag ccgccaacgg tcagcgcggc
    23701 tatctcatcg tcatctgttg cgagctggct tgcagcttca atggcattga gatcgaactg
    23761 gctgattttg gcgtcggcat tgtcgaaatt cagggtgtat tctggagtga caacaatgtc
    23821 ctgttcttca ggcaccagct taaagcaggt tattattttc atgatttctc ctgtgagtcg
    23881 catacggaac catctccgat ggaaggtaaa aatggcctga aaatagtgtg cgatagcggt
    23941 gcgggggggg gggcgacgga gcaagctgtt ttccttgcca acaatcttgc aaaaaaaatg
    24001 cgattgctaa aacaaaagtg caatttgcgg caatatagtt attccatgac cgtacaatct
    24061 tgcattcagt gtaaataatc ttgtttagct ctcgctaaac gcggttgtat aaccgcaata
    24121 cacgccgtat tttatccggc atatattgtt tttgcttgct catggaatag ggaggttgtc
    24181 atgtatcaac gctgttttga taatgccagt gaaacgctgt ttgtcgccgg taaaacgcca
    24241 cggctttcac gttttgcatt tagcgatgat ccaaaatggg agtctggaca tcacgttcat
    24301 gacaatgaaa ccgagctgat ttacgtcaag aaaggggttg caaggtttac catcgattct
    24361 tcgttatatg tcgcacatgc ggatgacatt gtggtgatag aacgcggcag gctgcatgcg
    24421 gtggcctctg acgttaacga tccggcaacg acgtgtacct gtgcgctgta cggctttcag
    24481 tttcaggggg ctgaggaaaa tcagttactg caaccgcatt cttgtccggt aattgccgcg
    24541 gggcagggaa aagaagtcat aaaaacctta tttaatgagc taagtgtgat tttgccgcaa
    24601 agtaaaaata gccaaacatc ttcgttatgg gatgcattag cctatacatt agc
//
LOCUS       DAJTSI010000053        24561 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000053 DAJTSI010000000
VERSION     DAJTSI010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24561
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.053"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(2..94)
                     /locus_tag="OO327_001901"
     tRNA            complement(2..94)
                     /locus_tag="OO327_001901"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO327_001902"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO327_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR6018455.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO327_001903"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO327_001903"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR6018456.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO327_001904"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO327_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR6018457.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO327_001905"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO327_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR6018458.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO327_001906"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO327_001906"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR6018459.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO327_001907"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO327_001907"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR6018460.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8017
                     /gene="srlA"
                     /locus_tag="OO327_001908"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7455..8017
                     /gene="srlA"
                     /locus_tag="OO327_001908"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8014..8973
                     /gene="srlE"
                     /locus_tag="OO327_001909"
                     /gene_synonym="gutE"
     CDS             8014..8973
                     /gene="srlE"
                     /locus_tag="OO327_001909"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR6018461.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8984..9355
                     /gene="srlB"
                     /locus_tag="OO327_001910"
                     /gene_synonym="gutB"
     CDS             8984..9355
                     /gene="srlB"
                     /locus_tag="OO327_001910"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR6018462.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9359..10138
                     /gene="srlD"
                     /locus_tag="OO327_001911"
     CDS             9359..10138
                     /gene="srlD"
                     /locus_tag="OO327_001911"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCR6018463.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10243..10602
                     /gene="gutM"
                     /locus_tag="OO327_001912"
     CDS             10243..10602
                     /gene="gutM"
                     /locus_tag="OO327_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCR6018464.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10669..11442
                     /gene="srlR"
                     /locus_tag="OO327_001913"
                     /gene_synonym="gutR"
     CDS             10669..11442
                     /gene="srlR"
                     /locus_tag="OO327_001913"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR6018465.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11435..12400
                     /gene="gutQ"
                     /locus_tag="OO327_001914"
     CDS             11435..12400
                     /gene="gutQ"
                     /locus_tag="OO327_001914"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR6018466.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO327_001915"
     CDS             complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO327_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR6018467.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14098..15537
                     /gene="norV"
                     /locus_tag="OO327_001916"
     CDS             14098..15537
                     /gene="norV"
                     /locus_tag="OO327_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR6018468.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15534..16667
                     /gene="norW"
                     /locus_tag="OO327_001917"
     CDS             15534..16667
                     /gene="norW"
                     /locus_tag="OO327_001917"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR6018469.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO327_001918"
     CDS             complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO327_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR6018470.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO327_001919"
     CDS             complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO327_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR6018471.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO327_001920"
     CDS             complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO327_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR6018472.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(20900..21019)
                     /locus_tag="OO327_001921"
     CDS             complement(20900..21019)
                     /locus_tag="OO327_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HCR6018473.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO327_001922"
     CDS             complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO327_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR6018474.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO327_001923"
     CDS             complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO327_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR6018475.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23682..>24561)
                     /locus_tag="OO327_001924"
     CDS             complement(23682..>24561)
                     /locus_tag="OO327_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="catalyzes the oxidation of formate to carbon
                     dioxide and molecular hydrogen; formate hydrogenlyase
                     comprises of a formate dehydrogenase, unidentified
                     electron carriers and a hydrogenase (subunit 3); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR6018476.1"
                     /translation="LIALGLVGGLYHLLNHSLFKSVLFLGAGSVWFRTGHRDIEKLGG
                     IGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQSFFKLSNSGAFVARLLGPLLAV
                     GLAITGALAVMCMAKVYGVTFLGAPRTKEAENATCAPLLMSVSVVALAICCVIGGVAA
                     PWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACPLLPFIIMAICKGDRLPSRSRGAA
                     WVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPVSLVPGWQCEGSALLFRRMA
                     LVELAVLVVIIVSRGA"
BASE COUNT         5796 a         6910 c         6297 g         5558 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatca tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 agcgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggccggcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata ccctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgccccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctactcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat atgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac accccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tacttttatt caaactttca aattaaaata tttatctttc attttacgat caaaataaca
     7381 cttttaaacc tttcaacctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggga tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgcaggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct actgttaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctgggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc
     7801 atatcaaccc tgcgaactgt ttgtttatct tggcattgcc agcggtctga caacgctgaa
     7861 cctgccactt ggcccactgg cggtgagtta tctgctggtt ggtctggtca ccaatttctt
     7921 ccgcggctgg gtgaccgatc tgaccaccgc catttttgag aaaaagatgg gcattcaact
     7981 tgaacaaaaa gttcacctgg caggagcaac atcatgacgc gtattcggat cgaaaaagga
     8041 acgggtggct ggggcggccc gcttgagctg gaagccacgc cgggcaaaaa aatcgtctat
     8101 atcaccgccg gtacccggcc tgcgattgtt gacaaactgg cacaacttac tggctggcag
     8161 gctattgacg gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc
     8221 gactgtggcg gcacattacg ctgcggcatc tatccgaaac gacgtattcc caccattaat
     8281 atccactcga cgggcaagtc cggtccgctg gcgcagtaca ttgtggaaga tatttatgtc
     8341 tctggcgtaa aagaagaaaa catcactgta gtgggtgatg cgacaccaca accctcttcc
     8401 gtgggccgtg actatgacac cagtaagaaa atcaccgaac aaagcgatgg tttactggcg
     8461 aaggtgggaa tgggcatggg gtccgccgtt gccgtgttgt ttcagtctgg tcgtgacacc
     8521 atcgacactg tattaaaaac cattctgccg tttatggcat tcgtctcggc gctcattggc
     8581 atcattatgg cttctggcct tggtgactgg attgcccacg gtcttgctcc gctagcgagc
     8641 catccactgg gtctggtcat gctggcgctc atctgctcct tcccactgct ttcacctttc
     8701 ctcggcccag gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt
     8761 ctcggcaata ttccgccgca tctggcttta ccggcactgt ttgccatcaa cgcgcaggcg
     8821 gcttgcgact tcatcccggt cggtttgtcg ctggcggaag cccgtcagga cacggttcgc
     8881 gtcggtgtcc cttctgtact ggtgagccgc tttttaaccg gcgcgccgac tgtactgatc
     8941 gcctggtttg tctccggttt tatctatcaa tagaggctga aacatgaccg ttatttatca
     9001 gaccaccatc acccgtatcg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat
     9061 cacctttcgt gaaggcgcgc cagcggacct cgaagagtat tgcttcattc attgccacgg
     9121 cgagttgaaa ggtgcactcc atcccggttt gcaattttca ctcgggcagc atcgctatcc
     9181 ggtgaccgct gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct
     9241 gcgcttcgat ggtttaaacg aagcggaatt tccgggcact gtccatgtgg caggccctgt
     9301 acccgacgat atcgcgccgg gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat
     9361 gaatcaggtt gccgttgtca tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg
     9421 tctggctgcc gaggggtatc gcgtcgcggt tgtcgatatt cagagcgaca aagccgcaaa
     9481 tgtggcacaa gaaattaacg ccgaatatgg tgaaggtatg gcgtacggtt ttggtgctga
     9541 cgccactagc gagcaaagcg ttctggcgct ctctcgtggg gtagatgaaa tctttggtcg
     9601 cgtggatttg ctggtctata gcgccggaat agcaaaagca gcctttatca gcgacttcca
     9661 gctcggcgat tttgaccgtt cgctacaagt gaatctggtg ggttatttcc tgtgtgcccg
     9721 tgaattttcg cgtttgatga tccgcgacgg gattcagggt cgcattattc agatcaactc
     9781 gaaatccggc aaagtgggca gcaaacacaa ctctggctac agcgcagcga aatttggtgg
     9841 cgtcgggctg actcaatcac tggcgctgga tctggcggag tacggcatta cggtgcattc
     9901 actgatgctc ggtaacctgc tgaaatcgcg gatgttccag tcactgttgc cacaatacgc
     9961 gaccaagctg ggtatcaaac cggatcaagt cgagcagtat tacatcgaca aagtaccgct
    10021 caaacgcggc tgcgattatc aagatgtgct gaatatgctg ctgttctacg ccagtcctaa
    10081 ggcgtcgtac tgcaccggac agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatc
    10141 aacagcggag atccgttaag gatctccgtg agactataga atgcctgatg cgctacgctc
    10201 atcaggcata caggacttcc gccactacat taaggaaaag ttatggtatc cgcactcatc
    10261 accgtcgccg ttatcgcctg gtgtgcgcaa ctggccttag gcggctggca aatttctcgt
    10321 tttaaccgtg ccttcgacac actatgccag caagggcggg ttggcgtgga ccgttccagc
    10381 gggcgcttta aaccgcgggt cgtggtcgcc atcgcgctgg acgatcagca gtgcatcgtc
    10441 gacaccttgt ttatgaaagg actgactgtc ttcgcccgac cgcaaaaaat tcccgcaatt
    10501 accggcatgc atgcgggtga tttacagccc gatgtgatct ttccccatga tccactatca
    10561 cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg tgaatgttac
    10621 ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt
    10681 cagcgtcagg ccgccattct ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa
    10741 ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt cattctggaa
    10801 catgccggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga ggaatccgat
    10861 ccgcctatcg atcataaaac gctcatcaac acccacaaga aagagctgat tgcagaagct
    10921 gccgttagtt ttatccatga tggcgattcg atcattcttg atgctggcag taccgttttg
    10981 cagattgttc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa cagcctgcat
    11041 atcgtcaatg cgctatccga actggataac gaacaaacta tcctgatgcc aggcggaaca
    11101 tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt cgagcatttc
    11161 accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacg
    11221 ttcaacgagg tttataccgt cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg
    11281 atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag tcttgaaagc
    11341 gtcgataagc taattaccga cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag
    11401 aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac tgaacacggg
    11461 acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccggaac gtctgggcga
    11521 tgattttgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg tggtttcggg
    11581 aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acacttgcca gtaccggcac
    11641 accggctttt tttgtccatc cggcagaagc actgcacggc gatctgggga tgattgaaag
    11701 ccgcgatgtg atgctgttta tctcttactc cggtggcgcg aaggaactgg atctgattat
    11761 tccgcgtctg gaagataaat ctatcgcgct gctggcgatg accggcaaac cgacgtcacc
    11821 gctgggcctg gcggcgaaag cggtgctgga tatctccgta gaacgcgaag cctgcccgat
    11881 gcaccttgcg ccgacctcca gcaccgtcaa taccctgatg atgggtgacg cgctggcgat
    11941 ggcggtcatg caggcgcgcg gttttaatga agaagatttt gcccgctccc acccagctgg
    12001 ggcactgggc gctcgcttgc tgaataaagt gcatcatctg atgcgtcgtg atgacgctat
    12061 cccacaggta gcgttaaccg ccagcgtgat ggatgcgatg ctggaactca gccgcaccgg
    12121 tctggggctg gtggcggtat gtgacgctca acaacaggta caaggcgtct ttaccgacgg
    12181 cgatttacgt cgctggctgg taggcggcgg cgcactcacc acgccagtta atgaagcgat
    12241 gacgacaggc ggcaccacat tacaggcaca aagtcgcgcc atcgacgcca aagaagttct
    12301 gatgaaacgc aaaatcactg ccgcaccagt ggtagatgaa aacggcaaac tcaccggcgc
    12361 aattaacctg caggatttct atcaggccgg gattatttaa tccttcaatc ccagacgttt
    12421 cgccagccga tgcaggttgg cgacgtcagt ttccagcatt cgcgcacagg ctgcccagtt
    12481 gtgatgattt tgtgccagtg cctggcgaat agtttcacgc tggaacgctt ctgtcgcttc
    12541 acgcaggttt tgcttaacaa cgggcaccgc cgccgcttct ggcggcggca acgtcacctc
    12601 aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgttgcggg tggctctcgc
    12661 cagcactacc gcccgatgaa tagcatgttc cagttcgcgc acgtttcccg gaaaactgta
    12721 gtgttgcagt aaatttcgcg ccccggcact taataccacg cgggagagcc cctgccgcaa
    12781 acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc gctcacgcag
    12841 cggcggcacc gaaagtggaa acacgctcag gcgatgaaac aaatcggcgc ggaatcgccc
    12901 tgccagcacc tcttcgcgta agtcgcggtt agtcgccgcc agcacgcgca catcgacccg
    12961 caaactacgg tcatcgccaa cgcgctgaat atcgccatac tgcaacaccc tcagcagctt
    13021 ggcctgcaat gccaacgaca actcgccgat ctcatcgaga aacagagtac cgttatccgc
    13081 catttcaaac ttcccgctgc ggttactgat agcgccagta aacgctcctt tcacatgccc
    13141 gaacaactca ctttccgcca cactttccgg cagtgcggca cagttgagat agaccagcgg
    13201 attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc accagctcct taccggttcc
    13261 cgtctcaccg ctgatcagaa cgttgagatc ggacgccgcc acaatctcaa tctctttttt
    13321 cagttgcgtc atgccagggg acaagccaat catctgcgtc tgtttcaccg cttcaaacgg
    13381 cgtggcatcg cctggcagca tattctggct ttccagttgt tcaatcagca acgcattgct
    13441 taacgctccc gccgccagcg cggcaatcag ccgtaactct tcgtcgctga aaacatcgaa
    13501 ctgatcgggc tgcatcccgt cgagcgtcag tgcgccgatc aggttttgtc cgacaaacaa
    13561 tggcagacca acgcaggcgt gaaccttcag actctcctgc ccaggaatca aaccgtcata
    13621 gggatcgggc aattcgctgt ctgcgggaaa gcgcaccaca tccccggcgc gggcaatcgc
    13681 ttccagccgt ggatgccctt ccagcgcaaa gcgtctaccg agtacatcct ttgccagacc
    13741 gtcgatggca agcggaataa actgccgcga atcgtaacgt agcaacgcag acgcatcgca
    13801 ctccagcacc tgacgtagcg tggtgatcag gcgctgaaaa cgatcctgat gaccaatccc
    13861 acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca tctttgcctc
    13921 actgtcaatt tgactataga tattgtcata tcgaccattt gattgatagt cattttgact
    13981 actcattaat gagcataatt ttatttatag agtaaaaaca atcagataag aaactggcac
    14041 gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga ggttgctatg
    14101 tctattgtgg tgaaaaataa cattcattgg gtgggtcaac gtgactggga agtgcgtgat
    14161 tttcacggca cggaatataa aacgctgcgc ggcagcagct acaacagcta cctcatccgc
    14221 gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga atttgtgcag
    14281 aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtgattaa ccatgcagaa
    14341 gaggaccacg ccggggcgct gaccgaactg atggcgcaaa ttcccgatac gccgatctac
    14401 tgtactgcca acgctatcga ctcgataaat ggtcatcacc atcatccgga gtggaatttt
    14461 aatgtggtga aaactggcga cacgctggat atcggcaacg gcaaacagct catttttgtc
    14521 gaaacaccaa tgctgcactg gccggacagc atgatgactt acctgacagg cgacgcggtg
    14581 ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt caacgatgaa
    14641 gtggatcaga cggagctttt cgagcagtgc cagcgttact acgccaatat cctgacgccg
    14701 ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg gctttaactt accagtcgat
    14761 atgatagcga cctcccacgg cgtggtctgg cgcgataacc cgacgcaaat tgtcgagctg
    14821 tacctgaagt gggccgctga ttatcaggaa gacagaatca ccattttcta cgacaccatg
    14881 tcgaataaca cccgcatgat ggctgacgct atcgcccagg gaattgcgga aaccgaccca
    14941 cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat cctgaccaat
    15001 gtcttccgct caaaaggcgt gctggtcggc acttcgacga tgaataacgt gatgatgccg
    15061 aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa acgcgccagt
    15121 gctttcggat ctcacggctg gagcggcggt gcgatggatc gcctttccac gcgcctgcag
    15181 gatgcgggtt tcgaaatgtc gctcagcctg aaagccaaat ggcgaccaga ccaggacgct
    15241 ctggagttat gccgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct cgcgccgctg
    15301 ccgcagagca cggtgaatac ggtagttaaa gaagaaacct ctgccacgac gacggctgac
    15361 ctcggcccac gaatgcagtg cagcgtctgc cagtggattt acgatccggc aaaaggcgag
    15421 ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag tcccggataa cttcctctgc
    15481 ccggaatgct ccctcggcaa agacgtcttt gacgaactag catcggaggc aaaatgagca
    15541 acggcattgt gatcatcggt tcgggcttcg ccgcccgcca actggtgaaa aatattcgca
    15601 aacaggacgc cactattcca ttaaccctga ttgccgccga cagcatagat gagtacaaca
    15661 aacccgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt acccgccaga
    15721 cggcgggtga atttgccggg cagtttaatc tgcgcctgtt tccgcacacc tgggtgacgg
    15781 atatcgatgc cgaagcccat gtggtgaaaa gccagaataa tcagtggcaa tacgacaagt
    15841 tagtgctggc aaccggtgcc agcgcctttg tcccgcctgt gcctgggcgt gagttaatgc
    15901 tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg gatgcccgac
    15961 gcgtgttgat tgtcggcggt ggtttgattg gtagcgaact ggcgatggat ttttgtcgtg
    16021 caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtca ttaatgccac
    16081 cagaagtaag cagccgcttg cagcatcggt tgacggagat gggcgttcat ctgctgttga
    16141 aatctcagtt gcaggggctg gaaaaaacgg attctggcat tctggcaacg ctggaccacc
    16201 agcgtagtat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg gaaacagccc
    16261 tggcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt tatctgcaaa
    16321 ccagtaatgc cgatatttac gcgctgggcg attgcgcgga aattaacggt caggtattgc
    16381 cgttcctgca gccgattcaa cttagcggga tggtgctggc aaaaaatctt ctcggcaata
    16441 acacgccgct gaaactaccg gcgatgctgg tgaaaatcaa aacgccggaa ttaccgctgc
    16501 atctggcagg cgaaacccag cgtcaggatt tacgctggca gattaatacc gaacgccagg
    16561 gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg gtcagtgagg
    16621 atcggatgaa agaggcattt gtattgttga aaacgttgcc ggtgtaggtg ggctactgtg
    16681 cctaaaatgt cggatgcgac gctggcgcgt cttatccgac ctacggggac gcatgtgtag
    16741 gccggataag gcatttacgc cgcatccggc aagggtgtcc aaatgcaaca cgctttatcc
    16801 gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc ccagagacaa
    16861 accgccatca cccgccggta aactctgtgg aaagagcaat gtgaaatcag cgagataatg
    16921 cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata ccagcgtagt
    16981 gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc
    17041 atgaaacgcc cacgcgcgtt gattaaccag tgcctgccag ttcagccact gctgccagaa
    17101 agtggcgaga tccagttgat tgtccacccg cggcatcgtc accggatgcg tcactccgtc
    17161 gcatgaggct gcgagcgcct ccagagcaca agccgcttca ccttcataac ttaacgtggc
    17221 tggcgcacag cccagtgccg ccgccactgc atcgaacaac cgcccacacg atgatgccag
    17281 cggcgcgtta attccacgct caatggcccg cgccagtacg ctccagtttt gctgttgcac
    17341 acttgccgtt tcagggtaat tctgccactc cggcacaaag cgcaggcact gcgccagcag
    17401 gtttcgccac ggctgcttcg ccgccaaatc gccacccgga agcgccactg caggcaagcc
    17461 gcccaggtgc tcacattcgc gatagttcac ccgcaggcac tcgccgcccc acaaagcgcc
    17521 attctccccc ataccgatac cgtcgagcgt caaagcaatg acatcaccgc catccagcgg
    17581 ccactgatgc tctgccagac acgccgctgc atgggcatga tgatgcagta ccgtttgcgt
    17641 cggcagattc atttcgctcg cccactggct ggagacatag cccggatgca catcatgcac
    17701 aatgtattgc ggagtgaaat cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg
    17761 catctggatg ccatcgtcac ttaaatcgcc cagatgctga ctcaacaccg cttgttcacc
    17821 gcgcaccagg cagaaggtat ttttcagatc cgcgccaaga cacagcacag gcggaacatt
    17881 tttaaagccc ggaggcaaaa ccagcgcatc cggcacatac ccccgcgaac ggcgcagcat
    17941 ttcgccgctt tcgcgcacca ctgaatcatc catccgctgc acgatgtcgc ggttatgtag
    18001 caagaatccg tcggcaatgc cctgcaaatc cgccagcgcc tgttcgttgc tgatagctgg
    18061 tggtttaccg ctcaggttgc cggaggtcat caccagcggg cattgcagtt cctgtaacag
    18121 caaatgctgg agcgggttcg caggcaacat caccccgact tcgttaaggc caggggcgat
    18181 atcatcacaa agctcaggta cgtatttttt atccaccagc acaatcggcg cagcgggcgt
    18241 ggtaagcaac tggcgcgcag cgtctggtaa actttctgcc actggcaaca tgactgccag
    18301 cggtttcgcc gggcgatgtt tgcgctcccg cagtgtcgcc accgcgttac tgttacgtgc
    18361 atcgcaggca agatgaaatc cgccaatccc tttgatggcg acaatgttgc ccatttttaa
    18421 ctgtgcgata gctgcctgta atgccgcctc ttgttctgca tgttcaccat gacttaccca
    18481 ttcaagatgc gggccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag
    18541 cgggtcgcgg tactctttgt cacaggccgg acatagcgga aacgccgcca tcacggtaaa
    18601 cgggcggtcg taaggcatcg cgcgaataat ggtgaaacgc gggccgcagt gggtacagtt
    18661 gataaacgga taacgataac gccgctcgcc tggggtattc atttcggcaa ggcaagcagg
    18721 gcaagtggcg gcatccggga caatttgcgt attcatggtg ccgccggtgc tctggcggat
    18781 ggtgaactcg gtgggcagtt gtgaccagat aaacggctca cgctcgacgc tatcaatacg
    18841 cgccagtggc ggacagtgct gatgcaattg aacaagaaac gtttccgggt cttccagcag
    18901 ccggacttct acgccatcgc cgtcattaca tacatcgccg tgaagattta attgctgtgc
    18961 cagctgccag acaaacggac gaaaaccgac gccctgcact ttgccacgaa tacgcagttg
    19021 gacaccgcaa gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc
    19081 cgccctgccg gacacgacag cgtcgtatcc ggcagtctca ggtcggcgat accgcccgct
    19141 ccgtattcta cgaatatttc cgggaattcc tttgatgcca gaacagttct gtaagatttt
    19201 tagaacatca gcgccgtacg gcggcgtttt tctgcgctca gttgttcaag tttattacga
    19261 tcgacacaaa tcagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcacga
    19321 tggttgcaca ggtcgcattt attggcttcg gctttgtcag cccgtacatt cagacccgcg
    19381 ccgctgttgc gaatcacagg acgtaccacc acttccatcg cgccatacgg gcaagccacc
    19441 acgcaggttt tgcaaccaat gcaacgttcc tgcatcacag gaacaaaccc tttatcacgg
    19501 ctgatagcac cattcgggca gacgttagcg cacggtgcat cttcacactg acggcaaact
    19561 gtcgccgtgg aaatgttcac acctttaatg acatggatac gcggtaaaaa tgtttccggg
    19621 gtcagcgatg cacagtcctg attttcctga tgagaaacca cgcacgccac ttcacaggta
    19681 cggcaaccaa tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc
    19741 aatgacagtt aatgcgccga tacactcaca aatcatgcca gtttttaatt tgctgttatt
    19801 taaggaaatt aatttctgta atgcaggaaa aaagatgtca tcgacactag tgacgatgac
    19861 atgtgatgac aatgtttatc gcgaaagggc aatgagtgag tcgcggcgga tcagttttcc
    19921 gctaaaggtt ttcggcggtg agaaatcccc gccatcgagc ataaaaatca gccgtccaat
    19981 aatttcctga atcatctcag tcaccggaat ttttacgctg gagagcgccg gaacgatata
    20041 gggggcaatt gcgatatcat cgaatccgat aactgacacc tgctctggca ccgctacgcc
    20101 gcgctcgtgc aacgctttga tcgcacctat cgccatatcg tcgttactgg caactaacgc
    20161 gctaaattta gcccctcgtt cgagcaacgt ttctacccct tcggccccgc tggcgggcgt
    20221 ccatttaccg ttagcgataa gtttttcatt gagcgcaata ccatgctgcg acagcgcgtc
    20281 tttatacccg gcaagacgtt caatgctggt gggggaatcc atcgagccgg taaggaaagc
    20341 aatctcctga tgcccggcgt ttatcaactc tgccacggcg ttaaagctgg tctgtttatg
    20401 atcgcaccag acgctatggc tgctattatt gcgcaggcgg cgattaagca ccattatcgg
    20461 ctgactgtgc gcgtcaatga tgtcatcaat ctcatccacg cttaaaaaac gcgggtaaat
    20521 cataatggcg tcgcagcgca aatccagcag atactgaatc gcctggcgct cttcttcggc
    20581 gctgtgttta ccatctgcca atagcaactg tcgccctttc tcttccgcca ttcgcgcggc
    20641 atgaaacagt aattcactaa aataaatgcc gtgataaagc gtgttggtca ctaccagccc
    20701 cagcgtctga gtactcttcg ccgacagatt gcgggccagc aaatttgggc ggtaactgct
    20761 ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc cattacctga
    20821 aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt
    20881 cgtcatcatt ttcatccctt tacacgcaat caacgcagtg tactgcaccg tttgccgatt
    20941 gtccttgcgc aatcggctcc agcggataag acgaggtcgc cgtgccggtt ttgatgactt
    21001 ttttgataaa ggtaaacatg gcgattcctt atttcagcgg cgagttttta cgctcaatgc
    21061 tgtaacgctc gagttctttg tacggcacca ctttgctctt cttcttacgc acatcgacta
    21121 cggtcatgcg gtcggtacag gagtagcaag ggtccaggct accgataatc agcggcgcat
    21181 aggaaacggt attgccacgc agcatgtagc gcagggtcgg ccagttcgcg taggtcgcgg
    21241 cacggcagcg ccagcggtac agcttctggt tgtcgccggt catgctccag tggatatcgt
    21301 cgccgcgcgg cgcttcggca aagcccagcg cgaaacggtg cggaatgtag gtaaagcctt
    21361 ccaccatcag cggaccgccc ggcaggttat ccagaccgta gtcgatcata ttcagcgcgg
    21421 tatagacctc gttgattcgc actttcagac gggaaataac gtcgcagccc tgctcgctgt
    21481 ggacttccat tggcagcagg ccataaccga caaacgggtg atcggcgcga gtatcgcggg
    21541 cgagaccgct ggcgcgaacc atcgggccaa cgttactgaa atcgcgggcg atttccgggt
    21601 ccagacgacc aatgccgacg gtgcgttgtt ccatgttcgg cgtactcagc agcacatcca
    21661 ccagctcctg cacttcacga cgcatttgtt gcgccagctg gcgggtctgg atcatgtcat
    21721 ctttcagcag atcgcgacga atcccgccga tcaggttcag gccgtaggtt ttacgcgcac
    21781 cggtaaggat ctctgccatt ttcatggagg tttcacgcac gcggaagaac tgcataaagc
    21841 cggagtcgaa gccggtaaag tgacaggcga ggccgaggtt gagcagatgc gggtgcaggc
    21901 gttccacctc cagcagaatg gcgcggatca tctgcgcgcg ttctggcacc tgaataccca
    21961 tcgcgttttc caccgacgtg gtgtaggcgg tgctgtgggc aaagccgcag atcccgcaca
    22021 cacggtcaga gaggaaggtc acttcgttat aacccatgcg ggtttccgcc agtttttcca
    22081 taccgcgatg gacgtagaac agacggtagt cggcgtcgat aatgttttcg ccatcgacga
    22141 acagacggaa gtgacccggt tcgtcagaag tgacgtgcag cggaccaatc ggcacgacgt
    22201 tgtttttctt gtcgcccagt tcgttgatga actcgtaggt ttcagcatcg gtagtcggtg
    22261 ccggacgctg acgataatcc atgctgtctt tacgcagcgg ataaagttca tccggccagt
    22321 catccggcag caccagacga cgttcatccg gcagaccaac cggaatcaaa ccgtacatat
    22381 cgcgcacttc gcgctcgccc cacaccgccg ccggaacgcg cggcgtcacg gacggatact
    22441 ccggtttgtt ggcgtcaact tcgacgcgaa ccgttaccca acacttggtg cccttctcca
    22501 tcgacagcac gtaataaacg gcgtaatgac cattcagttt gcgttcgtcg ttaccaaaca
    22561 gcaccgacag ccagccaccc tgcttgtagt agagaaactc caccacttcc ggcagatagt
    22621 tcacctttac ggtgacagtc agctgatctt tggtctgcca ggcgtggtcc agcacgacgc
    22681 ctagaaatgc ctcattcagc gcggcgagat aatgttgacc taatttttct tcagacatgc
    22741 tcaaactctc tttaatcacg ccgccagcaa ggagacgaac gctaaaaatg caaagccaaa
    22801 cccagcccag gtaatgcgcg gagtaatatc aagacgcaga cgggccatgc tgttttcgaa
    22861 cagcgcgata accaggacgc cgaccaccag tttgacgatg gcaatcacca gcgccagcag
    22921 cagtccaccg acggtgaagg tttccatttg tccccacgga ataaacaccc cgacgaacat
    22981 ctgcaacacc accagctgtt tcaggctgat accccatttc atgacgccaa agccgctacc
    23041 gctgtattcg gagagcggac cttcctgcaa ctcctgctcg gcttccgcca ggtcgaacgg
    23101 cagtttgccc atttcgataa aagtggcgaa cgcacaggcg cagagcgcca gcaccagcgg
    23161 gatgctttgg ctcagcggcc agtgataaac ggtgtcggta atgttgctga tgttggtgga
    23221 accggcaacc tgtgcggcaa cccacagacc aagcagcaac atcggttcga ccagcacgcc
    23281 aagcatcgct tcacggctcg caccgatagc ggtaaacggg ctaccggtat ccagaccgga
    23341 aatggcaaag aagaaacgcg cgatggcgaa gagatacagt aaggtgatca aatcgcccag
    23401 ttgcggcagc ggagaaccga cggtcaccac cggcagcgca gtagcgatag tcagcatgac
    23461 gcccaccatc acatacggcg tcaggcggaa catccagcca gaggcatccg ggccaacgct
    23521 ctggcggccc agcagtttga taatgtcgcg atactcctgc aacacgcccg gcccgcgacg
    23581 gttatgcaga cgggcgcgcg ccacgcgggt aataccggag agcagcggcg caacggcaaa
    23641 taacaccagc gcctgaatta acggatataa aacactcatt ctcaggctcc tcgtgaaaca
    23701 ataatcacca ccagtaccgc cagttcaacc agcgccatcc ggcggaacag caacgcacta
    23761 ccttcgcact gccagcccgg cactagcgac accggattca gccatttgcg cagtttcagc
    23821 actggcgcaa acgcctgttt aaccggcatg gcaaaaccgt gagcggtaat caccattgat
    23881 ttttcatggt cgtagccgca cacccaggcc gcaccgcggg aacgtgacgg caggcggtcg
    23941 cctttgcaaa tcgccataat gatgaatggc agcagcgggc aggcaatcag cagcaatgtg
    24001 atcattggtt gagaaacggt ggtgttagca ggctccagag gcagaggtac agcagcagag
    24061 agcatcggca gcagccacgg cgcagcaaca ccgccaatta cgcagcaaat cgccagtgcc
    24121 actacgctta cgctcatcag gagcggcgca caggtggcgt tttcggcttc tttggtacgc
    24181 ggcgcgccga ggaacgtgac gccatagact ttcgccatac acatcaccgc cagcgcaccg
    24241 gtaattgcca gacccacagc gagcagcggc cccagcagac gggcaacaaa cgcgccgcta
    24301 ttgctcagtt tgaagaagga ttgatagata acccattccc cggcaaaccc gttcagtggc
    24361 ggcagcgcag ccattgccat caacccgact aacatggcga tggagataac cggcattttc
    24421 ttgccaatac caccgagttt ttcgatatcg cgatgaccgg tacggaacca gacgctcccc
    24481 gccccgagga acagtacgct tttgaacagg ctatggttaa gcagatggta cagaccaccg
    24541 accaggccaa gagcaatcag c
//
LOCUS       DAJTSI010000054        24184 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000054 DAJTSI010000000
VERSION     DAJTSI010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.054"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(25..597)
                     /locus_tag="OO327_001925"
     CDS             complement(25..597)
                     /locus_tag="OO327_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCR6018477.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            815..1675
                     /gene="tesB"
                     /locus_tag="OO327_001926"
     CDS             815..1675
                     /gene="tesB"
                     /locus_tag="OO327_001926"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR6018478.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="OO327_001927"
     CDS             complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="OO327_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR6018479.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="OO327_001928"
     CDS             complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="OO327_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR6018480.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3559..5340)
                     /locus_tag="OO327_001929"
     CDS             complement(3559..5340)
                     /locus_tag="OO327_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6018481.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5333..7105)
                     /locus_tag="OO327_001930"
     CDS             complement(5333..7105)
                     /locus_tag="OO327_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6018482.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7135..7593)
                     /gene="decR"
                     /locus_tag="OO327_001931"
     CDS             complement(7135..7593)
                     /gene="decR"
                     /locus_tag="OO327_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCR6018483.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7747..8565)
                     /gene="cof"
                     /locus_tag="OO327_001932"
     CDS             complement(7747..8565)
                     /gene="cof"
                     /locus_tag="OO327_001932"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR6018484.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8665..10357
                     /locus_tag="OO327_001933"
                     /pseudo
     CDS             8665..10357
                     /locus_tag="OO327_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10422..11117
                     /gene="queC"
                     /locus_tag="OO327_001934"
     CDS             10422..11117
                     /gene="queC"
                     /locus_tag="OO327_001934"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR6018485.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11169..11567)
                     /gene="fadM"
                     /locus_tag="OO327_001935"
     CDS             complement(11169..11567)
                     /gene="fadM"
                     /locus_tag="OO327_001935"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR6018486.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11661..12032)
                     /locus_tag="OO327_001936"
     CDS             complement(11661..12032)
                     /locus_tag="OO327_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR6018487.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12183..14054)
                     /gene="ppiD"
                     /locus_tag="OO327_001937"
     CDS             complement(12183..14054)
                     /gene="ppiD"
                     /locus_tag="OO327_001937"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6018488.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14246..14518)
                     /gene="hupB"
                     /locus_tag="OO327_001938"
     CDS             complement(14246..14518)
                     /gene="hupB"
                     /locus_tag="OO327_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR6018489.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14727..17081)
                     /gene="lon"
                     /locus_tag="OO327_001939"
     CDS             complement(14727..17081)
                     /gene="lon"
                     /locus_tag="OO327_001939"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR6018490.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17269..18543)
                     /gene="clpX"
                     /locus_tag="OO327_001940"
     CDS             complement(17269..18543)
                     /gene="clpX"
                     /locus_tag="OO327_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR6018491.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18669..19292)
                     /gene="clpP"
                     /locus_tag="OO327_001941"
     CDS             complement(18669..19292)
                     /gene="clpP"
                     /locus_tag="OO327_001941"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR6018492.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19539..20837)
                     /gene="tig"
                     /locus_tag="OO327_001942"
     CDS             complement(19539..20837)
                     /gene="tig"
                     /locus_tag="OO327_001942"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR6018493.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21181..21498)
                     /gene="bolA"
                     /locus_tag="OO327_001943"
     CDS             complement(21181..21498)
                     /gene="bolA"
                     /locus_tag="OO327_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR6018494.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21803..22381
                     /locus_tag="OO327_001944"
     CDS             21803..22381
                     /locus_tag="OO327_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6018495.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22425..23900
                     /gene="ampG"
                     /locus_tag="OO327_001945"
     CDS             22425..23900
                     /gene="ampG"
                     /locus_tag="OO327_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR6018496.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5667 a         6666 c         5827 g         6024 t
ORIGIN      
        1 ccgggcggag agggttaacg tcgcttaata ttgcgtagga gcgggaactg ccgaagacgg
       61 attcacctga gttggtgaag tcgaaggtac ggtagtcgtt gcgccaccgc tggcctgaac
      121 cggcacggcg gtttgctgta ccggcaccaa tgtcaggtcg gctttagttc cgccctggtt
      181 gatcaccggc tgaacggtat cggtgataaa taccagtttg tcattcacgg taatcgccgc
      241 actcaacaga atacgcgcgt tcggctgaac atctgccggg ttaaatggca gaacaaagct
      301 gaatggtgac tgtttacctt cagtacgcac cgctttctgc gccagcactt ttgacggtgc
      361 atcggctaac gacgcgtcag aaagtgtcac ggtcagcaca gcatcaggcg gcagtgcgac
      421 tttctgacgg atccagacag taccggagac attcggttgc tggatagcag gttgttgtgt
      481 tgctgaaata gacgtatttg cagccggggc tggcgtctga atatccgcgc ttttatctgc
      541 gcaagccgcc aacgcaatcg caaccgctaa accactggcc atgtgcacga gtttcattca
      601 tttctcctta ttatcaatgc accagcgggc taactttcct cgccggaaga gtggttaaca
      661 aaatagtaac gtcaacaagt gtggcacaca tcacgcattt ctgcctgtaa ttagcccgta
      721 attcagacta tttcacccat cggaccagtg gcaaatttgc gttatactca actcactttg
      781 gcttgctgcg gcagctttgt tactggagag ttatatgagt caggcgctaa aaaatttact
      841 gacattgtta aatctggaaa aaattgagga aggactcttt cgcggccaga gtgaagattt
      901 aggtttacgc caggtgtttg gcggccaggt cgtgggtcag gccttgtatg ctgcaaaaga
      961 gaccgtcccg gaagagcggc tggtacattc gtttcacagc tactttcttc gcccaggcga
     1021 tagtaagaaa ccgattattt atgatgtcga aacgctgcgt gacggtaaca gcttcagcgc
     1081 ccgccgggtt gctgctattc aaaacggcaa accgattttt tatatgacgg cctctttcca
     1141 ggcaccagaa gcgggtttcg aacatcaaaa aacaatgccg tccgcgccag cgcctgatgg
     1201 cctgccttcg gaaacgcaaa tcgcccaatc gctggcgcac ctgctgccgc cagtgcttaa
     1261 agataaattc atctgcgatc gtccgctgga agtccgtccg gtggagtttc ataacccact
     1321 gaaaggtcac gtcgcagaac cacatcgtca ggtgtggatc cgcgcaaatg gtagcgtgcc
     1381 ggatgacctg cgcgttcatc agtatctgct cggttacgct tctgatctta acttcctgcc
     1441 ggtagctcta cagccgcact gcatcggttt tctcgaaccg gggattcaga ttgccaccat
     1501 tgaccattcc atgtggttcc atcgcccgtt taatttgaat gaatggctgc tgtatagcgt
     1561 ggagagcacc tcggcgtcca gcgcacgcgg ctttgtgcgc ggtgagtttt atacccaaga
     1621 cggcgtgctg gttgcctcga ccgttcagga aggggtaatg cgtaatcaca attaaaaaaa
     1681 tagccggagg tgaaaaccgt ccggctattt tttgcagtgc ttgttacgcg ttataggcat
     1741 tctcgccgtg gctgttgacg tccagccctt ctcgctcttg ttcttccggc acacgtaggc
     1801 caaccgtcag atccgccagt ttgtagccaa taaatgccac cacaccggac cagacgatcg
     1861 taatggcgat gctttccaac tgtaccagca actgatggcc catcgtcaca ccttcagcga
     1921 agcccacgcc gcccagagaa ctggcggcaa aaatcccggt catgatacag ccgacaatgc
     1981 cacaaacgcc gtgcacaccg aagacatcgc agggatcatc cacccgcagc aagcgtttga
     2041 gcatggtaac gccccacaaa cccgccagac cagctaccac gccgataatc aacgcgccgc
     2101 caaccccaat gtagccgcac gctggcgtca cgccgaccag accggcaatc gcgccagaac
     2161 acgctcccag cagagaaggc ttaccacgca gcgcccattc accgaagatc cagccaagaa
     2221 ttgccgccgc cgttgcgacc acagtattca caaatgccag tgccgcgatt tcattcgccg
     2281 tgcccactga cccggcgtta aagccaaacc aaccgatata gagaatggca gtcccggtga
     2341 agaccatcgg caggttgtgc ggtttaaacg cctctttacc gaagcctacg cgtttgccta
     2401 tcagatacgc gcccaccaga ccggcgattg ctgcgttaat gtgcaccacc gtgccacccg
     2461 cgaaatccag cgcaccgtga gaagccagca aaccaccgcc ccacaccata tgcgcaatcg
     2521 gaatgtaaga gagcgtcagc cataccacca cgaaaatcaa cacagctgag aagcggattc
     2581 gttccgccag cgccccaact atcaagccta cggtaatgca ggcaaacgat ccctgaaacg
     2641 ctacgtggat atactgataa atgctgccca tcaccgccgt cagttcgatg ttttttagca
     2701 tcaaccagtt aatgttgccg aagaagttgt tgccctcacc aaacgccagc gaataaccgt
     2761 aaaccaccca gagaatacag accagtgcaa acgtcaccgt cacctgcgtc agcatcgaca
     2821 gcacgttttt gccgcgaatc aacccaccgt aaaacagggc aatccccgga atagtcataa
     2881 acagcaccag cgcagtacaa atcatcataa acgcattgtc ggctttatcg gccaccgcag
     2941 gtgcagccat taccagtccc ggcagcatcg ccagtgaagc aagcccagtt tttatcgtcg
     3001 ctatcttcat ttttcgttcc tgttgctgtg tgccagagat tacagcgccg cttcgtcggc
     3061 ttcgccggta cgaatacgaa tgacgcgttg caattcagcg acgaagattt tgccgtcgcc
     3121 aatttttccg gtgtaagccg ccttactgac gatatcgatc acttcatcga gttggtcatc
     3181 agcaatcgcc acatcaattt ttacttttgg caggaagttg acgctgtatt ccgccccccg
     3241 gtacagctcg gcatgccctt tctgacgccc gaaacctttc acttcggtga cggtcaggcc
     3301 ctgaatacca atggaagata acgcttcacg aacgtcttcc agcttgaatg gtttgattat
     3361 cacggtcacc agcttcatag atcccctccg gtcagaattc ggtaatggtt ctgctacacg
     3421 aagaaggtat tgcaagcggt gtgccagaaa tgaaaaacga gaaagagagc aggaagttaa
     3481 gaagattgaa aatgaaaacg cactatgaca gtgcacagtg cgttacattt ttgcaccaac
     3541 gatgaaatat tgcgctattc aggcgctcaa tgattcctct tcacgcacgc tggctgccag
     3601 ctcttcgccc gcaagttgca gttgatacat ctgccagtag cgtccctggg ccgccagcag
     3661 ttgctggtga gtgccctgct ccacggcttg cccacgatga agcaccagaa tggtgtcggc
     3721 atcaataata gttgataagc ggtgagcaat caccaccagc gtggtatgtt cacgcaccgc
     3781 cgccagagca tgttgaatcg cctgttcagt accggagtca atgctggcgg ttgcctcatc
     3841 aaggatcagg atttgcggcg tctccaccag cacgcgcgcc agtgccagca gttgcttttg
     3901 cccgactgag agattatttc cctgctcgcc cagcggcgta taaatacaat cactcatgct
     3961 gcgcgccagc tccgccagtt gcacggtttc cagcgcctgc cagacgcgtt cttcggagat
     4021 atcccgcccc agcgtcacat tagcgaggaa ggtatccgcc agcaccaccg gatcttgctg
     4081 caccattgcc acgccctggc gcagcgcgct atgacttagc gaacttaatg gacgaccatc
     4141 aaggtgaatc tcaccttccg ttagcgggta atagcccatc aataaactgg cgagggtact
     4201 tttaccactg ccggtatgcc cgaccagcgc cacaaagtta cgcgaaggca cagagagatt
     4261 aatgttcttt agcaccagat tgtcatcgcg ataagcaaat gacacgttat cgacttcgat
     4321 ggtgccactc tgtaacgggt aatcatcatt gccatattgc tggcgcggtc cgtccatcag
     4381 ttcaaacacg cgctcaccag caacaacagc ctgttgcagc atcgcctgtt gcgtggtcag
     4441 ttcgattaag ggttcgttaa gtcgcccaag atagctgata aacgcataca acacgcccac
     4501 ttcaatggtg ccgctggcgg agaagccaaa cagcatcaat aagccacaaa gaatgagcga
     4561 tgaaaacaga ctcagcagcg gacgcagcag aaaaccgtcg aggcgcaggg tttgcatcct
     4621 agccatatag tgtgaacgac tggcctcccc catacgttcg ccaaatcgcg cctgctgacg
     4681 aaactgctgg ataacgctca tgccattgat gatttcgtta aagccgtcgt tgatatccgc
     4741 cagataggcg cgcacacggc ggacaatcgg cgtgctgtaa cgctggtata tcaccattac
     4801 caccagcacc accgggaaaa tcattatcgc caccagcgcc attcgccagt cgaggctgaa
     4861 catcgccacc agcatcgcgc ccaccagtgc ggcactgcgc aggacagttg ccactacggt
     4921 aacgtagaga tcgcggatca cttcagtgtc attagtgacg cgggaaatca cctgcccgac
     4981 gggttgggta tcaaactcgc ttaatggctg gcgtaacgca gcatccatca cgtcggtacg
     5041 caactgttgc actgcgccta ctgccgcccg attaaacagc agcgactgcg cgtaatgtag
     5101 cccggcggca aacagttgta gcccaacata cgccgcagcc agccctgcaa ccactttcaa
     5161 cggcaggtta tttttcgcta ccatattgtc gataaaatag ctgataagca gcggcccact
     5221 gacttctgcc gccgccgcaa cccacatcat caggaccgca atccccagcg gtttacgcca
     5281 cggcgaacca tacgctaaca ggcgcttgag agtcggccac agttggctaa aactacgcat
     5341 cgacggcctc ctcgcgaatt tccggaacgt cgtcgagcgc cgcctccagt tgttgatagc
     5401 gatacatatc gcgataccaa ccgctttgtt gcgccagctc atcatgattg ccacgctggg
     5461 cgatatgtcc gtgctgcatc acaataattt cactggcttc cgtcagtgcc gaaaggcgat
     5521 gggcactgat gattaccgtt cttccctgtc cccactgacg caggttatgc aggatctggt
     5581 gctctgtgcg tccgtccacc gccgaaagcg catcatcaag gatgaggatt tccgcgttga
     5641 ctaataacgc tcgagcaatg gagatacgct gtttttgccc accggaaagc atcacgccgc
     5701 gctcgcccac ttctgtatcg taaccttgcg gtagacgcaa aatatcgtca tgtacgctgg
     5761 ctaaccgcgc gacatgctca atctcttgct gggtggcatt cgggcaaccc agcgcgatgt
     5821 tattcgccac ggtgtcagaa aaaaggaatg gcgtctggct gaccaccgcc aggcggctac
     5881 gccagctatc gagttgtaac ttcgtcagag gaatatcatg aaagcgaata tccccctcgc
     5941 tgacgtcgaa atgacgctga atgagcgaca acagggtact tttgccggaa ccagtcggcc
     6001 cgcaaatacc cagcatctga ccgggtttca gggcgaaatt gacgttttcc agcgcaggat
     6061 ggtcagtctg cggatacgtg aactggtgaa tatttacatc cagttcgcca cgcccttccg
     6121 gcacgggttc gctaccatcg ttcaccaccg gcgcttccgc cagcatggcg cgaatacggc
     6181 tgtatgcagc actaccacgt tccacaatgt taaacatcca tgccagcgcc agcattggcc
     6241 aaatcatcag acctaaatac atcataaaac tggtgagctg gcccagcgtt aaactgccct
     6301 gcaccaccat ccaactaccg ccgccaatcg ccagcaagtt cgccatacca atcgcgatat
     6361 agatggtcgg gtcgaagcga gcatcaatac gcgccacccg catgtttttt ttgccggtat
     6421 cttcggcatc cgcggcaaat aacgccgact ggcgatcttc cagaccaaag gctttgatca
     6481 tgcggatact ggtgaggctt tcctgggtgc ggtcattaag actggaaaac gccgcctgtg
     6541 ccagcttaaa gcgttcatgc aaggcatcgc cgttgcgctt aatcatgatc gccatcactg
     6601 gcatcggtaa cagggcaaat aaggtcaact gccagctaat ttgcgtagac atcataatca
     6661 acacagcgca gcccattacc agtgaatcca ccagcgtcag caccccttct ccggcggcaa
     6721 acacgacgcg atcgacgtca ttggtcgcac gagccatgag atcacccgtg cgatgacgca
     6781 ggtaaaactc cgggtgctgc cggcttagct gacggtaata atcttcacgc agttcaacag
     6841 ccagttggta agacgcgcca aacagcaata cgcgccagac ataacgcagg agataaacca
     6901 caacggcaat cagcaccatg gtggcgatcc acatcaggct cagcccggta gtaaagtgtt
     6961 gttctgtcac gccatcgaca acaataccaa ccacttttgg cggaaccagt tgcagcatcg
     7021 cgataatgac aagcaaggcg acagccccaa gatagcgacg ccattcccga cggaaatacc
     7081 agcttaattg agcaaataat cgcacgcgtt gtgtcctgac ctgattctgg atatttattc
     7141 gatgggtaaa gaagtggtgt atttaatctg ttccatcgcg aagctggaag tgacgtccga
     7201 cagccccggc acgctgttta ccagacgctt ataaaactcg tcgtagcgtt tcatgtcggc
     7261 aacctggacg cgcatcagat aatcgtattc accagccatg cgccagaccc ccagcacttc
     7321 tggcatttcg gtaaccaccg tgacaaagcg gcaataccat tcgctgctgt gatgttgcgt
     7381 ttttatcagc acaaaggcgg tcaggccgag gcctattttt tccggatcca gcagggcgac
     7441 tttgccgata aggataccgt cgtcctccag ccgtttcagg cgcttccagc agggggtggt
     7501 tgtcagatta acggcttcag ccagtgcctg caaagagagg gtgcaatcct gctgcagtaa
     7561 ggccagcagc ttacggtcaa ttttatctaa catagccctt ccacggagaa tttttttctc
     7621 ttttaattct attttaaagg tgaaatcgcg acaatttatt ctgtgcggtt taaacaccac
     7681 ggcacaaaat gacaaattca acgcaaaaaa aaaccgggca attgcccggt gctggaaggg
     7741 atctcgttat tcgggggaat aaggtagatg tggatagtcc agccagtgcg tcaaatagtg
     7801 agagacagcc tgatttcggc aatgtccaat caccggtaaa tgcgggagct ccgcgcgcag
     7861 ttgcggcatc gcattgccca taataaaccc gctaccgacg ctgcctaaca tttcgcgatc
     7921 gttcatcgca tcaccaaagg ccatgcaatc gcgcaacgat aaacctaaat gttgggtccg
     7981 caccgtcaat gcagcgcctt tattgcagcc caccggcagc acttcgaggc aatccgtggc
     8041 ggaaaaacac aaatgtgcac gctcgcctaa tgcttcgtat agctggatct gcaagcgtgt
     8101 aagatcgtcg tgatcgccac agaagcagat cttggtgacg ctgccgagtg gcattttttt
     8161 gacatcgatt atctgataac gaaaaccgct atagacaaat acctgcaaca acgcagggct
     8221 ctctttcccg gtaaaccaac cgtcgtcatt gaagatatgc atgctggctc gggtatccca
     8281 ttgctgatac agcaccagct ccgcgacatc cgcaggtaaa tcatcacgat gtaaaagttc
     8341 accttccaga gaatgcacgc gcgttccgtt gccggtaatc aaatatgcat ccagcgatag
     8401 cgccccgaga atatgctgca tctccagcgc atgacgcccc gtggcaaaag tgagggtaat
     8461 gtcgcgttca cgcagtcgcg ccaaagtaga gagggttttc tcacctaaat gatggtcagg
     8521 catcaataaa gtgccatcca tatcaaatgc taccagacga gccatttctt tctccactct
     8581 gtgacacggt taatggtgat tgagtatcac ctgatatata cggaagtaat agtgaatagt
     8641 taaataatat tgttccgggt ttatatgcga ttgctcaacc gtcttaacca gtatcaacgt
     8701 ctgtggcaac cttcagccgc aaaccgtcac cgtcagcgaa ctggccgaac gctgtttttg
     8761 cagcgaacgc catgttcgta cgctgttgcg tcaggcacag gaggcgggat ggctggagtg
     8821 gcaggcgcag tcaggacgcg gaaagcgcgg acaattacgc tttctggtca cgccggaatc
     8881 gctacgcaat gcgatgatgg aacaggcact ggaaaccgga aagcagcaag atgtgctgga
     8941 gctggcgcaa ctggccccag gtgagctgcg cactctgtta cagccattta tgggcggaca
     9001 atggcaaaac gatacaccca cgttgcgtat tccctactat cgcccgctcg aaccgctaca
     9061 accaggcttt ttgcccggcc gtgccgagca gcatctcgcc gggcagatat tttccggcct
     9121 gacccgcttc gataataata ctcagcgccc gattggcgat ttagcgcatc actgggaaac
     9181 ctctactgac aggttacgct gggactttta tcttcgttca accctacact ggcataacgg
     9241 cgatgcagta aaagcctcac acttacacca gcgattattg atgctgttac aactgccagc
     9301 actggatcaa ttatttatta gcgtgaagcg tattgaagtc acccatccgc agtgtctgac
     9361 cttcttttta catcgccccg attactggct tgcgcaccgg ctggcgagct attgcagcca
     9421 tctggcgcat ccgcaattcc cactgatcgg cactggtcct tttcgcttaa cacaattcac
     9481 agcggagctg gtgcgcctgg agagccatga ttattaccat ttgcgtcatc cgctgcttaa
     9541 agcggttgag tactggataa ctccgccgct tttcgaaaaa gatttgggaa ccagttgtcg
     9601 gcatcccgtg caaatcacca tcggcaaacc ggaggagctg caacgggtca gccaagtcag
     9661 tagtggcatc agtttaggtt tttgctattt aacgttgcgc aaaagtcccc gactctccct
     9721 ctggcaggcg cgaaaagtga tctccattat tcatcaatcc ggtttattac aaacgttaga
     9781 agtcggagaa aacctgatca ccgccagtca tgcattactg ccaggctgga ctattccgca
     9841 ctggcaagta ccggatgaag tcaaactacc gaaaaccttg acgctggttt atcacctacc
     9901 gatagaactt cataccatgg cagaacgcct acaggcgaca ctggcagcgg aaggctgtga
     9961 actcacaatt atttttcata acgcaaaaaa ctgggacgac acgaccctac tggcacacgc
    10021 agacctcatg atgggcgaca gattaattgg cgaagcaccg gaatatactc tggagcaatg
    10081 gctacgttgc gatccactat ggccacatgt tttcgacgct ccagcatatg cacatctgca
    10141 atcgacgctg gacgcggtgc aagtaatgcc tgatgaggaa aaccgattta atgccctgaa
    10201 agcggttttt agccagttaa tggcagacgc gacgctgacg ccgctgttca actatcacta
    10261 tcgcattagt gcccctcccg gcgtgaacgg tgtgcgactg acaccgcgcg gctggtttga
    10321 atttaccgaa gcctggcttc ccgcgccgtc gcaatgaata gctggtccgg gttaatcgca
    10381 ggcgttacca taacgctttt attgttcaag caggattatc tatgaaacgt gctgtcgttg
    10441 tgttcagtgg aggccaggat tccaccacct gtctggtgca ggcattacaa caatatgatg
    10501 aagtccattg cgtgacgttc gattacggtc agcgacatcg cgcagaaatc gacgtggcac
    10561 gcgaactggc gctgaaactg ggagcacgcg cgcataaggt tcttgatgta acgctgctca
    10621 acgagctggc ggtcagcagc ctgacacgtg acagcattcc ggtgcctgat tatgaacctg
    10681 aagccgatgg catcccgaat acgtttgtcc cagggcgtaa tattttgttc ctgacgctgg
    10741 cggcaatata tgcgtatcag gtaaaagcag aagccgtaat taccggcgtc tgtgaaacgg
    10801 atttctccgg ctacccggat tgccgcgatg agtttgtgaa agcactaaac catgccgtca
    10861 gtttgggcat ggcgaaagat attcgttttg aaacgccgct gatgtggatt gataaagcgg
    10921 aaacctgggc gctggcagat tattacggca aactggattt agtccgtaac gaaacgttga
    10981 cctgctataa cggcattaaa ggcgacggtt gcggtcattg tgcggcatgt aatttacgcg
    11041 ccaacggttt gaatcattat ctggccgata aaccgacggt gatggcagcg atgaagcaga
    11101 aaaccgggtt gaggtaatta ttccgggtgt cgccggatgc ggcttgagca tccggcacca
    11161 caaaacgttt acttaaccat ctgctccagc ttttcgcgca attccccttc cagagctaat
    11221 gctttctgcg ttttaagatc aatacaaaca aacgtaataa gcgcatccgc taccacctgc
    11281 ccttccggct ccagtgtaat gacctggttt aagatgccgc ttttaccgtt taattgctgc
    11341 aactgactgg taatagttaa caggtcgctt aataccgctg gacgacgata gttaatattg
    11401 atattgacca cgacgaaggc gatgttatgg gccgtcatcc actgaaaact gtcgctattt
    11461 tccaccccat cccagcgggc ttcttcgaga aattcaaggt agcgggcgtt gttgacgtgc
    11521 tggtaaacgt cgagatgata tccacgaact ttgatttgtg tttgcatagc gcaataacct
    11581 tacgttgttg ttataagtac agaggtcata actggtatga cctcttcagt ctggcaaaaa
    11641 attgccacta tgcaaattaa ttacagggtt aataccgcca gattacgttc caccagcgaa
    11701 ttgcccatcc ccggcacctg ctttagatcc tccaccgttt taaacggacc gtactcttcg
    11761 cgataactga caatcgcctg cgctttcttc aggccaacgc cattcatcgc gcgggctagc
    11821 tcttccgcgc tggcattatt aatgctgacc cgggtgcctt cttcgtcact ggctttcgcc
    11881 ggtactgctg ctttactttg tgctgcagga gcttccgctt tggtttctac cgccgtcggt
    11941 ttcgccacag aagctgccgc cagcgcgcta tgagacattc cggcacaggc cagggacagt
    12001 gtaatgagca gtgctttaat tccgtgtttc atactgtttt ctccttgttt gttaacagtg
    12061 gaatcacctt aacgggagtg aaaacagtaa acaaatggca atgttcagaa atggaaaagg
    12121 ccgcgaaagc gaccttttct tgttacagct gcaacgcatt tttttgcgag gcggcttcag
    12181 gattattgct gttccagcgc atcgccaatt ttgattttcg cctctttacg caggttactc
    12241 atcagagctt caaagacgat ttgtgcgttg ttctgggtga taccctgcac catcgctttt
    12301 ttctgatctt ccggcattga accttgtttc acttcatcca gcgccagcag aaccacatta
    12361 ccttgcatat cggtcgccat accgtagctc ggtttgtctt tcgctggcag tggcagtgca
    12421 aacgccgcct ggctaatcgg gtcacgaccg gaacggctta aggttttcgg ctcgccaaat
    12481 ttcagaccgg cagcctgcat agcttccgca cctttgccgg ctttcaaatc aaccagcagt
    12541 ttctcagcat ccactttcgc ctgttgttca gctttgttgt gctgaaccag tgccttaact
    12601 tgttcctgaa catctgccaa cggtttcacc gcttccggtt tgtgctcgct gacgcgcagc
    12661 acgaatgcgc ggtcgccgtc tacggtgatg atgtcagagt tgatgcccga cgcgccgttt
    12721 tcacctacca gaccgccgtt aaagatagcg tcggcaaccg gcttgaagtt caactcttcc
    12781 ggcaggttat ctttgctgaa ccaacccgtc tgagtggctt taacgccggc agcttgctct
    12841 gcaccggcca gagactcggt gtcgttgctt gccgcatcgc tcactttctg ctgcagcgca
    12901 tagtacgcat cgagggcttt ttcgtgtttc actttcgccg caatgtcgtc acgtacttcg
    12961 tctaacgatt tcactttcgc tggctgaatg tcgtccagac gtacaatcag gaaaccgacc
    13021 gaagatttga tgacaccaga cagttggcct ttttctttca gaccagcatt tttcagttcg
    13081 tcggggatag tggcatcttc taaccaaccc atatcgccgc cgttacgggc agagataata
    13141 tcggcagatt tttctttggc taatgcagca aaatcaccgc ctttattcag ctcatcaagt
    13201 accgctttcg cttcatcttc agttttggtc tggatgatgc tgtagcgggt acgctgcggc
    13261 tgggtgaatt gatcctgatg ctgatcgtag tagctctgga tatccgcatc gctaaccggt
    13321 tgctgcatcg ttgcggcaac cagcttgatg taactcacgc ggaattgttc cggcgtcatg
    13381 aaattgtttt tgttttgttc gtagtagctg gcaatctcct gttcggtcac aggctgcttc
    13441 gccgccagcg cgttaacatc gatagtcgcc tcacgcacca cgcgttgttg cgcgaccagt
    13501 gccgccagct cgtcggtttc acctttcagc ataaaatcgg taccggcaac gccgttaatc
    13561 agctgttggg tagtgagctg gttacgcagc gcctgggcgt actgatcggc ggtcatcccc
    13621 atctggttga ggataccgtt atagcggctg ttatcaaatt tgccgtcaac ctggaaggct
    13681 ggggtcgcga aaatcgcctg tttaacctgc tcatcactga tacccagttt cagctcacga
    13741 gcgtactgat ccaacagcgc ctcgtcgatc agacgattca gcacctgttg acgcagggtt
    13801 ttcatatagc cttcgttcgc tgccagctcg gagtattgat cgcccagctg ttgctgcata
    13861 cgattacgct cgctgttaaa ggcgttctcg aactgcccac ggctgatttc ctggtcattc
    13921 acttttgcgg cgtagttatt gcctccgcca atcaggtaac cactcacgcc ggtcaatatg
    13981 aacgacacga taatgatacc gaaaataatc ttgagcacga gactgtttgc agccgtgcgt
    14041 aagctgtcca tcatggtgta acaacactcc gctgtagatg actgtgtacc tcacgcaacg
    14101 tatcgatgcg cgcaaaggcc tattgtgaca agaaacgggg gcaattgtca gcccacaaga
    14161 cacagaaagt caccgaatac aaataaaaaa ggcacatcag tagatgcgcc cttgaacttc
    14221 gtcacatccc cactggggac aacgcttagt ttaccgcgtc tttcagtgct ttacctgcac
    14281 ggaagctcgg tactttagcg gcagcgatgg tgatctcttt accggtctgc gggttgcggc
    14341 cagtacgggc agcacgctct ttaacggcaa aagtaccaaa acctaccagt gctacatcat
    14401 ccccttcttt cagagattca gttacggaag caataatagc atctaacgca cggccagccg
    14461 cagctttaga gatatcagcc cctgcagcaa tcttgtcgat caattgagat ttattcactc
    14521 ttctcttcct ctttataatt tatatcgcac ttgaatcctt caaggtacga acgcgcagca
    14581 gttatatcag gccagccatc cccttacaac accccttaat aagggcaagc ccgatccgct
    14641 atctaactta gcgagacaaa aaaaggctgg caagcccgaa ttagcctgcc agccctgttt
    14701 ttattagtgc attttgcgcg aggtcactat tttgcagtca caacctgcat gccagacggt
    14761 tcattttgca gcgccagagt cagaacttcc tcaatgcgct tcacaggatg aatgtccaga
    14821 tcggcaatta cgttgtcagg aatctcttcc agatcgcgtt tattttcgaa cggaattagc
    14881 actgttttaa tcccgccgcg atgcgctgcc aggagttttt ctttcaaacc accgatcggc
    14941 agtacctgac cacgcagagt gatctcaccg gtcattgcca catcggcacg aaccgggtta
    15001 ccggtcaggc aagaaaccag cgcggtgcac atagcaatac cggcactcgg accatctttc
    15061 ggcgtcgcac cttccggtac gtggacgtgg atgtcacgtt tttcgtaaaa atcagggttg
    15121 atccccagtt tttccgcacg cgcacgaacc accgttaacg ccgcctgaat agactcctgc
    15181 atcacttcgc cgagcgaacc ggtataggtc agtttgcctt tacccggaac acatgcggtt
    15241 tcaatggtca gcaagtcacc gcccacttcc gtccacgcca gaccgattac ctgaccgaca
    15301 cggttttcgt tatcagcgcg accatagtcg aaacgctgaa caccgaggta gtcatgcagg
    15361 ttatcgccgt taatttcgat atgttttaat gacttatcga gcagtaactg cttaaccgct
    15421 ttgcggcaca gtttggagat ttcacgctcc agaccacgca cgcccgcctc acgggtgtag
    15481 taacgaataa tgccgataat ggcgctatcg tcgacggtca gctcaccttt tttcagtgca
    15541 ttacgttcaa tctgcttcgg cagcaggtga cgtttggcga tgttcagttt ttcatcttcg
    15601 gtataaccgg agaggcgaat cacttccata cgatccagca gcggtgccgg aatgttcatg
    15661 gagttcgacg tcgcgacaaa catcacgtcg ctgagatcgt aatccacttc caggtagtgg
    15721 tcgctgaacg ctacgttctg ctctggatcc agcacttcaa gcagtgcaga ggccggatcg
    15781 cctcgcatgt cagaagacat tttgtcgatc tcatcgagca ggaacagcgg atttttcacg
    15841 cccactttcg ccattttctg gatcagttta cccggcatag aaccgatgta agtacggcgg
    15901 tgaccacgga tttccgcttc atcacgcacg ccgcccagcg ccatacggac atatttacgc
    15961 ccggtggctt tggcaatgga ctgaccaaga gaggttttac ctacccccgg cggccctacc
    16021 aggcagagga tcggtccctt gattttgttg acacggcttt gaaccgcaag gtactcaagg
    16081 atgcgatctt tcacgcgctc cagaccataa tggtcggtat caaggatttc ctgcgcctga
    16141 cgcaggtctt ttttgacctt gctgcgcgca ttccacggta cctgtaccat ccagtcgata
    16201 taaccacgca ctacggtcgc ttctgccgac atcggagaca tcattttcag cttctgcaac
    16261 tctgcttccg ctttctcttt tgcctctttc ggcatcttcg ccgcgtcgat tttgcgcttc
    16321 agggcttcgt tttcgtccgg cacgtcgtcc atttcaccga gttctttctg aatagctttc
    16381 atttgctcgt tcagatagta ctcacgctgg gatttctcca tctgcttttt aacgcggttg
    16441 cgaatgcgtt tctcaacctg cagcagatcg atttccgatt ccatcattgc catcagatat
    16501 tccagacgtt cgttaacgtc ggacatctcc agaacggact gtttgtcagc cagtttcagc
    16561 ggcatatgtg cagcaatggt atccgccaga cgcgccggat cgtcgatgct attcagcgac
    16621 gtcagcactt ctggtgggat ttttttgttc agcttgatgt agccttcgaa ctggctgatt
    16681 gcagtacgca ccagcacttc ctgttcccgc tcatcaatgg tcggcgactc cagatactcc
    16741 gccttcgcag aaaagtgttc gccattgtca gagagcgcag aaatacgcgc gcgctgtaac
    16801 ccctcgacca gcactttgac ggtgccgtca ggcagtttca gcatctgcaa tatagaggcc
    16861 acggtcccga cggtgaaaag atcgtttaca cccggctcat ccgttgaagc ttctttctgc
    16921 gcgaccagca taattttttt atcatggtcc atcgccgctt ccagacaacg gatagatttt
    16981 tcccgcccga caaataaggg gatgaccatg tgcggataaa ccaccacatc gcgcagcggc
    17041 aatacgggga tttcaatgcg ttcagaacgc tcaggattca tagagctctc tcttagttta
    17101 atttccgcca ggtaatcaga tgacacgact gtgcttcacg ccatctatta acatgtacgt
    17161 cagtatatgg ggatgtttcc cccacattca acgccgagaa tagaggaaaa attaaagggg
    17221 agataaaatc cccccttttt ggttaactaa ttgtatggga atggttaatt attcaccaga
    17281 tgcctgttgc gcttccggct taccataaat cagcaacggt ttgctttgac catcaattac
    17341 cgactcgtcg ataaccactt tttcgacgtc ttccatggac ggcagatcgt acatggtatc
    17401 gagcagtgcg gcttctacga tggaacgcag gccacgggca ccggttttac gcgccatcgc
    17461 tttcttagcg atagcatcca gcgcctcgtc acggaattcc agatccacgc cttccagatt
    17521 aaacagcgcc tgatactgct tggtcagggc gtttttcggc tctttgagga tctgaatcag
    17581 agcttcttcg ctcagttcat tcaacgttgc tacaaccggc agacgaccaa tgaactcagg
    17641 gataagacca aacttgatca gatcttccgg ttcaacctgc gccagcagct cgccttcgct
    17701 tgctttgtcg gacttcgctt ttaccgtcgc gccaaaacca atgccggagc cagtttctac
    17761 acggtgggaa atcactttat ccagaccggc aaacgcaccg ccacaaataa acaggatctt
    17821 agaggtatca acctgcaaga attcctgctg cggatgttta cgcccacctt gcggtggaac
    17881 agcagctacc gtaccttcga tcagtttcaa cagtgcctgc tgtactcctt caccggaaac
    17941 gtctcgggta atggacgggt tgtctgactt acgagaaatc ttgtcgattt catcgatgta
    18001 gacaatcccg cgctgcgctt tctgtacgtc gtaatcgcac ttttgcaaca gcttctgaat
    18061 gatgttttca acgtcttcgc ccacataacc ggcttcggtc agcgtggttg cgtcggccat
    18121 ggtgaacggg acgtccagca ggcgcgccag cgtttcagcc agcagcgttt taccggaacc
    18181 ggtcggaccg atcagcagaa tgttactttt gcccaactcg acaccattgc tggtatcgcc
    18241 gttgcgcaga cgtttgtaat ggttgtatac cgcgaccgcc agcacttttt tcgcctgttc
    18301 ctggccgata acgtaatcgt ccaggtggtt gcgaatttca tgcggcgtcg gtagcgcact
    18361 gcgttcacga tgcggtgcaa cttctttaat ctcttcgcga atgatgtcgt tacataaatc
    18421 aacacattcg tcgcagatat acacggatgg accggcaatc agcttgcgca cttcatgctg
    18481 gcttttgccg caaaaagagc aatacagcaa tttgcctgag ccatctttgc gtttatctgt
    18541 catgggtcaa aacctcttct ttgttctttg tgccgcacac gacgacgcaa atgccattct
    18601 caggcgcaag ccgcttacag cgttgtgccg ccctagataa gtatagcggc acagttgcgc
    18661 ctctggcatc aattacgatg ggtcagaatc gaatcgacca gaccgtattc caccgcttca
    18721 ggggcggaaa ggaagcgatc gcgctcggta tcacgttcaa tctgttctaa tgattgaccc
    18781 gtatgaagcg ccataagttc attcatgcgc cctttaactt tcagaatttc acgggcatga
    18841 atttcgatat cggtcgcctg gccctggtag ccgcccaacg gttggtgaat catcacgcgc
    18901 gaattcggca ggcaaaaacg tttacctttt gcccctgcgg tcaacaagaa agcgcccatc
    18961 gaggccgcct ggcccataca gatggtgctg acatcaggct tgataaactg catggtgtca
    19021 tagatagaca tcccggcagt gatcaccccg cctggggagt taatgtacag atagatatct
    19081 ttttctgggt tttccgcttc caggaacagc atctgcgcca caatcaggtt agccatgtgg
    19141 tcttcaacct ggccagtcag aaaaatgacg cgttccttaa gtagacgaga atagatatca
    19201 aaagagcgct caccgcgtga ggtctgttca atgaccatcg gcaccagcgc catatggggt
    19261 gcaaagttat ctcgttcgcc gctgtatgac atttccgtct cctggataaa attgaaaaaa
    19321 acctgctgta ccgattgtaa ccttatggac ggattatcag ctagtccctg tgttatgggt
    19381 acggcatcac catatttcaa gcataacaat cttttgttgt tacgctaaca ccgaaagagg
    19441 cttttcgcac ggctccatgc aaaattcatg acaaaaaaaa gcccaccacc tggcggtgac
    19501 gggcctttgt gcgaatttag cgcgttatgc tgcgtaaatt acgcctgctg gttcatcagc
    19561 tcgttgaaag tggtttcttt ttcagtcact ttcgctttcg ccagtacagc ttcaacagcc
    19621 tgttcttcca gagcaacatt gcgcatgttg tccatcagtt ctttgttttt gctgtagaac
    19681 tcgataactt ctttcggatc ttcgtacgca gaagccatct cttcgatcag gcctttcacg
    19741 cgctcttcgt cagctttcag ctcgttggtg cggataactt cgcccagcag caggccaaca
    19801 actacgcggc gtttagcctg ttcttcgaac agttcgcgcg gcagttccag agcttgtttt
    19861 tcgttgccac cgaaacgctg tgcagcctgg cgacgcagaa cgtcgatttc gctgtcgatc
    19921 agcgcagccg gtacgtcgat gtcgttagct tttaccagac cttcgatcgc ctgagactta
    19981 acgcggttac ggatggcgct cttcagctcg cgctccatgt ttttacgcac ttcagcgcgc
    20041 agaccttcta cggaaccatc ttcaacgccg aaacgtttga tgaattctgc agtcagttcc
    20101 ggcagttcac gctcttcaac tttcttcagg ttgatagcga atttcgctgc tttacctttc
    20161 aggttttctg cgtggtattc ttccgggaag gtcacgtcga tggtgaactc ttcgccagct
    20221 ttgtggcctt tgataccgtc ttcaaagccc gggatcatac gaccctggcc catcgccagt
    20281 acgaaatcag acgctttacc gccttcgaac tcttcgccgt ctacagaacc ggtgaagtcg
    20341 atggttacgc ggtcttctgc ttcaacagcg ccgtcttttt ctttccaggt cgcctgctgt
    20401 ttacgcagag tatccagcat gccgtcaacg tcagcgtcgg tcacttcaac gatcggtttt
    20461 tcaacttcga tcgcttccag accctgcagt tcaacttccg gataaacttc aaactctaca
    20521 gagtaagtga agtcttcacc cagcttgtat tcgcccggaa cataagtcgg tgcgccagcc
    20581 ggattgattt tttctttaat gatggcgtca atgaagttac ggctcatcag gtcacccaga
    20641 acgtcctggc gtacagacgc gccataacgc tgagcaacga tattcattgg cactttgcct
    20701 ttgcggaagc cgtcaatacg tacttttttc gcaacgttga ccagctcgct tttaacagcg
    20761 gtctcgatgc tgtcagcagc gatagtaatc gttacacggc ggccaaggcc ttgagtggtt
    20821 tcaactgaaa cttgcatctt gttacctcaa aaaatcacag tgctcggtca actcttaccc
    20881 cgcaagcaca ggttgttggc ttcgcggaac cgggatgttc ttataatcaa tcacattccc
    20941 tgttaccaga atcatcccga aagcattcaa ataggacgcg gcattatagc ggcatcactt
    21001 ttatgagtcg agaacggtta ttgcgcgctg tcgcgtattt tttcacaatt cccgggcaat
    21061 tttcgtctga atgctgggca aaaagcgcaa aatccagaca aaacaaaacg gcccgcaggc
    21121 cgtttttcat aaaatcttta gcaacatact ggaaaagcgc cgacagttgc aaatgcgttt
    21181 ttacgcgatg cttcctgctc cacgacaggg aggagaggca aagacggtgt cctgcaaccc
    21241 ttcccactcc ttaatagtgt aagtatgcag agccagcgca tgaacggtag tagagagttc
    21301 ctccgctaaa gtactgtaaa tcattcgatg acgattcaga aaacgttcac ccgtaaaacg
    21361 atcgctgacc agcacaactt taaaatggct ttcagagccg gctgggacat tgtgacgata
    21421 gctttcatcc actacttcga ggaatacggg ttggaacgcc gcccttaatt tttcttctat
    21481 ccgctcacgt atcatcatga aatatctcct ccgacaacgc tgagatgtca tccatccctt
    21541 taaatactag ccgcttttac cgtttccatt gcagcttaac aacaaatatt taggtttcgt
    21601 cctggttttt ccgtcaagcg tatgaagttt actgatttaa agattgaaac acaggcctcg
    21661 catcagcgtt tcttttacca ggggtgatta tttcgtcaac atgatgaatt tacatgcctt
    21721 acccacttcc cctcgccgtt ggcaatgtta tgatggcggg aatttttacc aaacacgctt
    21781 aagagtctct gagagcccga acatgttcaa aaaaatcctc ttcccgttag ttgctctgtt
    21841 tatgcttgca ggatgcgcaa aaccgccaac aactattgaa gtttccccga cgattacact
    21901 gccacagcag gatccaagcc tgatgggcgt caccgtaagc attaatggtg ccgatcagcg
    21961 taccgatcag gcgctggcaa aagtcacccg cgataatcaa atcgttaccc tgaccgcctc
    22021 ccgcgctctg cgtttcctgc tgcaagaagt gctggaaaaa cagatgaccg cgcgtggtta
    22081 catggttggt ccgaatggcc cggttaatct gcaaatcatc gttagccaac tgtatgctga
    22141 cgtgtcccag ggcaacgtgc gctacaacat cgctaccaaa gcggatatcg ccatcatcgc
    22201 taccgcgcag aatggcaaca aaatgaccaa aaactatcgt gccagctaca acgttgaagg
    22261 tgcgttccag gcctccaaca aaaatatcgc cgatgcggtt aacagtgtgc tgaccgatac
    22321 catcgctgat atgtctcagg acaccagcat tcacgaattc atcaagcaga acgcgcgtta
    22381 atttctgccc tctggcccgg tgcaagccgg gcctgtagac gcccatgtcc agtcaatatt
    22441 tacgtatttt tcaacagccg cgttcagcca tattgctgat cctgggtttt gcttccgggc
    22501 taccgctcgc cctgacatcc ggcaccttac aggcctggat gacggtcgaa aatatcgatc
    22561 tcaaaaccat tggtttcttc tctctggtag gccaggctta cgtttttaaa ttcctctggt
    22621 caccgctgat ggaccgctac acgcccccat tttttgggcg gcggcgcggt tggctgctcg
    22681 ccacgcaaat cctgttatta gtcgccattg cggcgatggg ttttctcgaa ccaggcaccc
    22741 aactccgctg gatggcggcg ctggcggtgg tgatcgcttt ttgctctgcc tcccaggata
    22801 ttgtcttcga tgcgtggaaa accgatgtgc tcccggcaga agaacgtggt gcgggcgcgg
    22861 caatcagcgt gctgggttac cgtttaggga tgctggtttc cggcggccta gccctgtggc
    22921 tggcagataa atggttgggc tggcagggca tgtactggtt gatggcggcg ctgttgatcc
    22981 cctgtattat cgcgacgttg cttgcaccag aaccaaccga caccattcct gtgcccaaaa
    23041 cgctggaaca agcggttgtt gcacctctgc gagatttctt tggtcgcaat aatgcctggc
    23101 ttattttgct tcttatcgtg ctgtataagc tgggcgacgc attcgccatg agcctgacaa
    23161 ccacgttttt gattcgcggc gtcgggtttg atgcgggtga agtaggcgtg gtaaacaaaa
    23221 cgcttggctt actggcgacc attgttggcg cattgtacgg tgggattttg atgcagcgcc
    23281 tgtcgctgtt ccgggcgctg ctgattttcg gcattttaca aggtgcgtct aacgctggtt
    23341 actggctgct gtcgattact gataagcatc tctacagcat gggcgcagcc gtctttttcg
    23401 aaaacctctg tggcgggatg ggcacatcag cctttgtcgc gctgttaatg acgctgtgta
    23461 ataagtcatt ttccgctact caatttgccc tgctctcagc gctttccgct gtagggcgag
    23521 tttatgtcgg ccccgtggcg ggttggtttg ttgaagcaca cggctggtcg acattctatc
    23581 tattctccgt cgccgctgcc gtaccggggc ttattttgct gctggtttgc cgccagacgc
    23641 ttgaatatac acgagtaaat gacaacttta tctcccgtac cgaatatccg gcaggttatg
    23701 cctttgccat gtggacactg gcggcgggcg tcagcctgtt ggccgtgtgg ttacttctgt
    23761 tgacgatgga cgcgctggat ttgacgcact tctctttcct gcctgctctg ctggaagtcg
    23821 gggttttagt cgccctttct ggcgtcgtgc ttggtggttt gctggattat ctggcgctac
    23881 gaaaaacgca tctgacgtaa tctgtaaata ttatttagct gtagtaatca ctcgccgtaa
    23941 ttattacggc taaataaaca tcagcatcgc ttattaatat ttgttctttt gtgcggctta
    24001 gcgtttggaa attattgacg ccatttataa attctatttt tcttacacga ttcagctaat
    24061 gagtctttat tttctcacca cccagttgtc actttaatga taattatttg ttaaataatt
    24121 gttttatttc gcattggtta taccaattgc ccaccaagac tccttctgca ctcccctttt
    24181 gtta
//
LOCUS       DAJTSI010000055        24089 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000055 DAJTSI010000000
VERSION     DAJTSI010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.055"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..178
                     /gene="yjbD"
                     /locus_tag="OO327_001946"
     CDS             <1..178
                     /gene="yjbD"
                     /locus_tag="OO327_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCR6018497.1"
                     /translation="VLTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEA
                     SFSWSANTSTRGRR"
     gene            complement(311..1183)
                     /gene="rluF"
                     /locus_tag="OO327_001947"
     CDS             complement(311..1183)
                     /gene="rluF"
                     /locus_tag="OO327_001947"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR6018498.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1531..2478
                     /locus_tag="OO327_001948"
     CDS             1531..2478
                     /locus_tag="OO327_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCR6018499.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2548..3354
                     /locus_tag="OO327_001949"
     CDS             2548..3354
                     /locus_tag="OO327_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6018500.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSENGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3364..3771
                     /locus_tag="OO327_001950"
     CDS             3364..3771
                     /locus_tag="OO327_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCR6018501.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3771..4265
                     /locus_tag="OO327_001951"
     CDS             3771..4265
                     /locus_tag="OO327_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCR6018502.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4331..5128
                     /locus_tag="OO327_001952"
     CDS             4331..5128
                     /locus_tag="OO327_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR6018503.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWCKAEPQLQATASTALDQLDD"
     gene            5139..5927
                     /locus_tag="OO327_001953"
     CDS             5139..5927
                     /locus_tag="OO327_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR6018504.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5979..7208
                     /gene="sorE"
                     /locus_tag="OO327_001954"
     CDS             5979..7208
                     /gene="sorE"
                     /locus_tag="OO327_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCR6018505.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            7590..8279
                     /gene="pepE"
                     /locus_tag="OO327_001955"
     CDS             7590..8279
                     /gene="pepE"
                     /locus_tag="OO327_001955"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR6018506.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8370..10001)
                     /locus_tag="OO327_001956"
     CDS             complement(8370..10001)
                     /locus_tag="OO327_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR6018507.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10221..13904)
                     /gene="metH"
                     /locus_tag="OO327_001957"
     CDS             complement(10221..13904)
                     /gene="metH"
                     /locus_tag="OO327_001957"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR6018508.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEQGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADALEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14104..14928
                     /gene="iclR"
                     /locus_tag="OO327_001958"
     CDS             14104..14928
                     /gene="iclR"
                     /locus_tag="OO327_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR6018509.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            15245..17429
                     /gene="espL4"
                     /locus_tag="OO327_001959"
                     /pseudo
     CDS             15245..17429
                     /gene="espL4"
                     /locus_tag="OO327_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(17398..19135)
                     /gene="aceK"
                     /locus_tag="OO327_001960"
                     /pseudo
     CDS             complement(17398..19135)
                     /gene="aceK"
                     /locus_tag="OO327_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(19319..20623)
                     /gene="aceA"
                     /locus_tag="OO327_001961"
     CDS             complement(19319..20623)
                     /gene="aceA"
                     /locus_tag="OO327_001961"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HCR6018510.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(20653..22254)
                     /gene="aceB"
                     /locus_tag="OO327_001962"
                     /pseudo
     CDS             complement(20653..22254)
                     /gene="aceB"
                     /locus_tag="OO327_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(22523..23452)
                     /gene="metA"
                     /locus_tag="OO327_001963"
     CDS             complement(22523..23452)
                     /gene="metA"
                     /locus_tag="OO327_001963"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR6018511.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            23609..24052
                     /locus_tag="OO327_001964"
     CDS             23609..24052
                     /locus_tag="OO327_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR6018512.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         5944 a         6146 c         6109 g         5890 t
ORIGIN      
        1 cgtactgacc aactccgaaa ccaacagcat taagaaagag tacatcgata aattaatggt
       61 cgagcgtgag gctgaagcga aaaaagcccg ccaattgaag aaaaagcagg cttataaacc
      121 ggatcctgaa gcgtcattta gctggtcagc aaatacgtcg acgcgcggca ggcgctaatg
      181 aacaatcaac gcctgagatt ttcactcgtc tgtgtggaca caatcttccc ctcaccctaa
      241 ccctctcccc gaaggggcga ggggactgtc cgagctcttt ttggacgttt tcatcagtct
      301 gaggcgttaa tcagcgtccc ttctttttac gccccggcga ggtaaaacgc ttaccgttgg
      361 acgccgggcg accgcctttt tccgccgttt tttccatctt aacgactgga cgtttgatgc
      421 ccgctgtttt cggtttggcc ttcgctttag gttttgcctc ggaagaggaa ttttcgatga
      481 gcttaaagag gtcgattaac tcatcgtcgg ttaaatcgcg ccactccccc agcggaatgc
      541 cgcttaagct gacgttcata atgcgcgtac gttccagctt tttcacttca tagccgaaat
      601 gctcgcacat gcgccggatc tgacggttca gcccctgcac tagggtaatg cggaagacaa
      661 acggcgcttc ttttttgact ttgcactttt tcgtcaccgt cccgaggatc ggcacccccg
      721 cccccatacc acgaataaat tcatcggtaa tcggtttatc gaccgtcacc agatactctt
      781 tctcatgatc gttacccgca cggaggatct tattcaccag atcgccgtga ttggtgagga
      841 aaatcagccc ctgggagtct ttatccaggc ggccaatcgg gaacacgcgt ttgctgtggt
      901 taacgaaatc gacaatgtta tcgcgctcgc catcttcggt ggtgctgaca ataccgacgg
      961 gcttgttcag ggcgataagt accaaatctt cggcttcccg aggctcaatc aactgaccgt
     1021 ttactttcac aatgtcgccg ggtttcacct gatcgccaat ggtggctcgc ttgccattaa
     1081 ggaacacatt gccttgctcg atatagcgat ccgcttcgcg gcgtgagcaa attccgcttt
     1141 cgctgatgta tttatttaaa cggactgatg agtcgggcag cataaattct cctgtaaaac
     1201 gcggaatata ccgtacctct tagcggggaa aaacagggtt atgcggcaac gtcgaaatta
     1261 aatgtgatct gccacgcctt ttatcacaaa agtgtctccg gaagtacgat gcaaatttgc
     1321 ctggcaaaat aaggataatc aacatattac gaatcgctgc acacctgtgc tgatgactac
     1381 tacgcagaga aacaaatgtg caagagaaag cgctatttct ccccatgcaa agagtgaaaa
     1441 atcgctttga aaggccctca tgatggggtt atatcagatc aagcggtgag aaagcgtatt
     1501 tgcacaaatg tgcagaacca ggaggcggca atggaaaaca gtgacgatat ccgtttgatt
     1561 gtgaagattg cccaactcta ttacgaacag gatatgacgc aggcgcaaat cgcgcgcgaa
     1621 ctgggtattt accgcaccac catcagccgc ttgcttaaac gaggccgcga tcagggaatg
     1681 gtcaccatcg ccatcaacta tgactacaac gaaaatctct ggctggagca gcaactgaag
     1741 caaaagtttg gcctgaaaga cgttgtggtg gtgtcgggaa atgatgagga tgaagagact
     1801 caactggcga tgatggggtt acacggcgcg caactgctgg atcgcttgct ggagcctggc
     1861 gatattgtcg gtttttcctg gggtcgcgcg gtgagcgcac tggttgaaaa cttgccgcag
     1921 gcggggcaat cgcggcagtt aatctgcgta ccaattattg gcggaccgtc cggtaaactc
     1981 gaaagccgct atcatgtaaa cacattaacc tacagcgcgg cagcgaagct gaaaggggaa
     2041 tcgcatctcg cggattttcc ggctctgctg gataacccat taattcgtaa tgggatcatg
     2101 cagtctcagc actttaaaac catctctgcc tactgggata atctggatat cgccctggtg
     2161 ggaattggct caccggccat tcgcgacggc gctaactggc atgcgtttta tggtggtgaa
     2221 gagagtgacg acctgaatgc ccgccaggtt gctggcgata tttgctcgcg cttttttgat
     2281 attcacggcg caatggttga aacgaatatg agcgaaaaaa cgctctctat cgaaatgaat
     2341 aaattaaagc aggcgcgata ttccattggc attgccatga gtgaagaaaa atacagcgga
     2401 attattggtg cactgcgtgg aaaatatatt aattgtctgg taacgaatag cagcacagct
     2461 gaactattac tgaaataaaa atcttcccga tgtttaccgt aataaaaatt acggcgggtc
     2521 cctattatct aaaatcagga gtctgttatg caaacgtggt taaatttgca ggataaaatc
     2581 attattgtca ccggcggcgc atccggtatt ggtctggcga ttgtagagga attattagca
     2641 caaggcgcga atgtacagat ggtcgatatt cacggtggcg atggtcaata tgaaggtcat
     2701 aaaggctatc agttttggcc gaccgatatt tctagcacaa aagaggtaaa tcatacggta
     2761 gcggaaatta tccagcgttt tggtcgcatc gacggtctgg tcaataacgc cggggtcaat
     2821 ttcccgcgtc tgctggtcga tgagaaagcg cctgccgggc agtatgaact caacgaagct
     2881 gcattcgaaa aaatggtcaa tatcaaccag aaaggtgttt ttctgatgtc gcaggcggtg
     2941 gcgcgacaga tggtcaaaca acatgatggc gtgattgtga atgtttcctc agaaaatggg
     3001 ctggaaggct cagaaggaca aagctgttac gccgctacca aagccgcgct caatagcttc
     3061 acgcgctcct ggtcgaaaga gctgggtaaa cacggtatcc gtgtggtcgg tatcgcgccc
     3121 gggattctgg aaaaaaccgg gctacgcacg ccggaatatg aagaggcgct ggcgtggacg
     3181 cgcaatatca ccgtcgagca gctgcgtgaa ggctatacca aaaacgccat tcctattggg
     3241 cgcgccggaa gattagcaga agtagctgat tttgtttgtt atctgttgtc tgaacgcgcc
     3301 agctacatca ccggagtaac cactaacatt gcgggcggca aaacgcgcgg ctaaggaggc
     3361 tttatggtca acgccatttt ttgtgcccac ggcaaattag cttgcgccat gctggaatcg
     3421 gttcagatgg tttacggcaa cgccaatgtt gaagccgttg catttgtgcc cggtgaaaac
     3481 gccggagaca ttgcggtaaa gctggaaaag ttagtaagca ttcacaatca agatgagtgg
     3541 ctgattgccg tggatttaca gtgcggcagc ccgtggaatg cggcagcaat gcttgcgatg
     3601 cgcaatcctc gtttgcgggt aatcagcggc ctttctctgc cgctggcgct cgaactggtt
     3661 gataaccagg acagcatgaa tgttgatgaa ctgtgtgaac acctgacgca aatcgcaaag
     3721 caaacctgcg ttgtctggaa acaactggca acgacagagg aggatttctg atgaacatta
     3781 cgctcgcccg cattgatgac cgtctgatcc acggccaggt cactaccgtc tggtcgaaag
     3841 tggctaacgc ccagcgcatt attatctgca atgacgaagt ttataacgat gaagtgcgcc
     3901 ggacattatt gcgtcaggct gcgccaccgg gaatgaaggt taacgtggtc aatattgaaa
     3961 aagccgtcgc ggtttatcac aacccgcaat atcaggacga aaccgttttt tatcttttca
     4021 ctcgcccaca ggatgcctta gcgatggttc gccagggggt aaaaataggc accctgaata
     4081 ttggcggcat ggcctggcgg cccggtaaaa aacagttaac caaagcggtt tcattagatg
     4141 atgatgatat taacgcattt catgagttaa ataatcttgg tgtcatactg gatttacgcg
     4201 tcgtagcgtc agatccctca atcaatatta tcgacaaaat aaacgaacag ttaatcgcaa
     4261 attaaataat agcgcctgtg tacatatgct ttaacaggca gggatgacga cacttaaaag
     4321 gtgacaaatc atggaaataa gtaccctaca aatcattgct atatttcttt tttcctgtat
     4381 tgccggaatg ggcagcgtgc tggatgaatt tcagactcat cgcccgttaa ttgcctgtac
     4441 ggtgattggt ttaattctcg gtgatttaaa aaccggaata atactcggcg gtacgctgga
     4501 attgatagct ctctgctgga tgaacgtcgg cgcggcgcaa tctccggatt ctgcactcgc
     4561 cagcataatc tccgccattc tggttatcgt tggtcagcaa agcatcgcca ccggaatcgc
     4621 catcgcgttg cctgtggctg cggcaggcca ggtgctgacg gtgtttgccc gtaccatcac
     4681 cgtagtgttc cagcacgcgg cggataaagc agcagaagag gcgcggtttc gcactctcga
     4741 tattctgcat gtctccgcgc ttggcgtgca ggcgctgcgc gtcgccattc cggcactgat
     4801 tgtctcactg ttcgtcagcg ccgatatggt cagcaatatg ttaaacgcca ttcccgaatt
     4861 tgtgacccga ggactacaga ttgctggcgg ttttatcgtg gtggtcggtt acgccatggt
     4921 gctacgtatg atgggcgtga aatatttgat gcctttcttt ttcctcggtt tcctcgcagg
     4981 tggctacctc gatctcagcc tgctggcgtt cggtggcgtc ggcgtgatca tggccctggt
     5041 ctacatccag ctaaatccac agtggtgtaa agctgaacct caactccagg ccaccgcctc
     5101 caccgccctt gaccaacttg acgattaacg gagccatcat ggaacagaga aaaattacac
     5161 gcagcgatct ggtgagcatg tttctgcgct ccaacctgca acaggcgtcc tttaactttg
     5221 aacgtattca cgggctgggc ttttgctacg acatgatccc cgccatcaag cgactttacc
     5281 cattaaaaga ggatcaggtt gcggcgctca ggctacacct ggtgttcttc aataccacgc
     5341 cagccgtatg tggcccggtc atcggcgcgg aaattgatga cggtaccatc aacggcatca
     5401 aagtcggtct gatgggacca ttggcaggag ttggcgatcc actggtctgg ggaacgctgc
     5461 gcccgattac cgccgcgctc ggcgcatctc tggcactttc gggcaacatt ctcggcccgc
     5521 tgctgttctt ctttattttc aacgcggtgc gtctggcgat gaagtggtat ggcctacagc
     5581 tcggctttcg caaaggggtg aatatcgtca gcgatatggg cgggaatgtg ctgcaaaaac
     5641 tcaccgaagg cgcgtcgatt ctcggactgt ttgtgatggg cgtgctggtg accaaatgga
     5701 cgtcaatcaa cgtaccgttg gtggtttcac aaacgcatgc cgccgatggc tccaccgtca
     5761 ccatgaccgt gcagaacatt ctcgaccaac tttgccctgg tttgctggcg ctcggtctga
     5821 cgctactaat ggttcgtctg ctcaacaaaa aaattaaccc ggtatggctg attttcgccc
     5881 tgtttggctt agggattatc ggcaatgctc tgggcttcct gtcctgattc ttcgccccgg
     5941 cacgactgcc gaggccatcg ctcaacatga ggtggtttat gaaaacaaca gctctgcgtc
     6001 tttatggtaa acgtgattta cgcctggaaa cctttgacct tcctgaaatg caggaggatg
     6061 aaatcctcgc aacggtggtc actgacagcc tgtgcctctc ttcctggaaa gaggccaatc
     6121 tgggtgaaaa ccataaaaaa gtacccgacg atgtgacgac caacccaatc atcatcggcc
     6181 acgaattttg cggcgatatt ctggccgtgg gtaaaaagtg gcagcacaaa ttccagccgg
     6241 gtcagcgtta tgtgattcag gccaacctgc aactccccga ccgcccggat tgccccggct
     6301 actccttccc gtgggtagac ggcgaagcga cgcatgtggt tattcccaac gaggtcatgg
     6361 aacaatattg cctgctggca tacgacggcg aaacctattt tgaaggctca ctggttgaac
     6421 cgctttcctg cgtgattggc gcgttcaacg ccaactatca tcttcaggaa ggtagttata
     6481 accacacgat ggggattcgc ccgcaagggc acacgctgat cctcggcggc accggaccaa
     6541 tgggactgtt ggcgattgat tatgcgctgc atggaccagt taacccgtcg ctgctcgtca
     6601 ttaccgatac cgacaacgac aaattgagtt atgcgcgcaa gcactatcca tcagaaccgc
     6661 aaacactgat tcattatctc aatgccaccg atgcagcatt tgatacgtta atggcgctga
     6721 gtggcggtca cggcttcgat gatattttcg tctttgtgcc taatgaagga ctggtgactc
     6781 tcgcctcttc cttgctggcg acagatggtt gcctgaattt cttcgccgga ccgcaggata
     6841 aacatttcag cgcgccaatt aatttctacg atgtgcatta tgcatttacc cactacgtgg
     6901 gcacgtcagg cggcaatacc gacgacatgc gcgcggcggt caaactgatc gaagagaaaa
     6961 aagtgcaggc cgcaaaagtg gtaacacata ttcttgggct gaatgccgcg ggcgaaacca
     7021 cgcttgaatt gcctgccgtc ggcggcggca aaaagctggt gtataccggg aaatacctgc
     7081 cgctgacgtc actcacgcag attcaggatc aagcactggc ggcgattctg gcgcgtcatc
     7141 aggggatctg gtccggtgag gtggagcaat acctgctcgc ccatgcagag gcaatttccc
     7201 atgattaatc gcgctactta actctgcatg tcgctggctg gtcgccccag caacttcgcg
     7261 accggatgtg cggctgattg ctgaagcagc acaatttgca cgagaaggat aaaaaatatc
     7321 agcgccttaa ggggcgctca aattggtggc aaagtacaca ttgtttatgc cggatgcgga
     7381 atgaagcctt attcggtcta cataattttg aaaatccaat atattgcagt tatctggtag
     7441 gccaggtaag cgtagcgcat caggcaatgc tgtgtttgtc atcagtctca gcgcccggtg
     7501 caatacgtga catcgtgagc catgtcagta agtggttaaa ttcgcagtgg tagcatgcct
     7561 tccgtgattt cttactaacc ggagaagtaa tggaactgct tttattgagt aactcgacgc
     7621 tgccgggtaa agcctggctg gaacatgcac tgccgctaat tgctgaacag ttgcagggtc
     7681 gccgctcagc ggtgtttatc cctttcgctg gcgtaacgca gacctgggat gattacacag
     7741 agaaaacggc tgcggttctc gctccgctgg gtgtttctgt caccggtatt catagcgttg
     7801 tcgatcccgt tgccgcgatt gaaaatgcgg aaattgtgat tgtcggcggc ggtaatactt
     7861 tccagttgct gaaacagtgc cgcgagcgcg ggctgctggc tccaattact gacgtggtta
     7921 aacgtggcgc gctgtatatt ggctggagcg caggcgctaa ccttgcttgc ccaaccattc
     7981 gtaccaccaa cgatatgccg attgtcgatc cgcaaggttt cgatgcgcta aatctgttcc
     8041 cgttgcaaat caacccgcac ttcaccaacg cgctgccgga aggccataaa ggtgaaaccc
     8101 gtgagcagcg tattcgcgaa ctgctggtcg tcgcgccaga actgacgatt attggtctgc
     8161 cggaaggtaa ctggatcaca gtgagtaaag gtcacgctac gctgggtggc ccgaacacca
     8221 cttatgtgtt taaggctggt gaagaagcgg ttccgctgga agctggtcac cgtttttaag
     8281 tgaattgccg gatgcatgat ccggcatctt gttcatagtg cctgatgcga cgcttgacgc
     8341 gtcttatcag gcctacagga ttcacaatat taatactcgt cccgtccttc atcttcatcc
     8401 ggctgttcca gcacactgta agccaccgaa caaaacagcg agttcaggcg ctgcatatct
     8461 cccagtaagc ctaaatgtag cgaactggtt tcaatgctct gcacgttttg ctgatgcagg
     8521 cgatcgacgt gggcgtggga atagcggcga ttaagaatgc gaaaacgatg tttgctgcga
     8581 cgcaaacgac gagcgctggt gacatcgcca gagaagaaca ccgacattgc cagctttaaa
     8641 ttactgagca attgctcata gagcgcatcc agttccttca acccatcaag cgaaaatgcc
     8701 cgccgtgctg ccagcgattt atcggcaatt tcgctgccca tgcgctcgac gatatcggag
     8761 gcctgttcaa ggttgagcga catttcgatg atctccgccc agcggcgcga ctcttcctcc
     8821 gccaactctt cttttggcat ccgcgccaga tacagcttaa tggcggtgta gagaacgttg
     8881 atatcatctg ccagcttacg cagctctttc tcctgccgtg gctcgccgtg catcacttta
     8941 ttcagccctt ccatcatctg ttccatggcg tcgccaatgc gcagggtttc gcgcgcggcg
     9001 ttcgccagag caagcgtggg cgtgtccagc gcgctgacat ccagatgttt aggccgtagc
     9061 tgggtatcca gttccggttc atcgcgaata atcgttttgc aaaaccgtgc catcgggtca
     9121 acaaatggca gcatgaccag gcaacgtaca aggttgtaga agacgtggaa atagatcacc
     9181 agttccgctt ttggcaatga cagcttcccc attgtctctg ccagcaaatg gacaaacggc
     9241 aggataatca ggctacccac cagcttaaac agcagactac ccagcgcgac acggcgggct
     9301 gcggcattgg cggcactgtt gttgagcatc gccagcaggc cggaaccgag gttagctcca
     9361 atcaccagac agagcgccac ggggaaggag ataatgcctg cagcggtcag agtcgcggtc
     9421 agcagtacag cagcaaggct ggagtagctg ataatggcga acatcgcgcc aatcagcgca
     9481 tccagcagaa tatcgccggt cagcgaggca aagatcacct gaacgccgtt tgcctgggtg
     9541 atcggcgtta cggcctgcac aatcaactcc agcgccagca aaatcagccc aagaccaata
     9601 ccgacgcggc ccagttgccc ggcgcgtgac tgtttgcgtc cgaggaaaaa aatcacgccg
     9661 ataaaaataa gtaacggtga cagccaggat aagtcgaagg tgagaatacg cgccattagc
     9721 gccgtcccga catctgcccc cagcacaatg accagagccg gtgcgagggc taccagatcc
     9781 tgagcgacga acgaggtcac cagcatggtg gtggcattac tgctctgtac cagtgcggta
     9841 acgccgatcc ccgcgcaaaa ggcgagcggc tttttttcga cgctccggct aaggacagta
     9901 cgcaaacgcg cgccgaagac gcgcattacg ccggttcgaa caatatgagt cccccagacc
     9961 agcagggcga cggcagaaag caggtgaagc agcgttaaca cgagttcaga ttctccttat
    10021 cgttatatgt tcctgaagca attgaatgac caaaggaccg gaaacacgct gactggcatc
    10081 tttactgtgc cttttgacat tcgtttttcc ggctaagatt attccagcct gtttccagta
    10141 taagtgtttc atattaataa agagacaggg cactcattga gcgccctgtt tgttgtaagg
    10201 aaaagtgaca gatttgtgaa tcagtccgcg tcatacccca gattcggtgc cagccagcgc
    10261 tcaacatcgg agacgctcat acctttacgg cgggcatggt cttcaacctg atcgcgctga
    10321 atttgcgcca ccgcgtagta cttgctgtcc ggatgactga aataccaacc cgaaaccgat
    10381 gcaccaggcc acatcgcgaa agattctgtg agtttcatgc cagtatgttt ttccacttcc
    10441 agcagctccc agatggtggc tttttccgta tgttccgggc aggccggata gcccggtgcc
    10501 ggacggatgc cctggtagtt ttcgcggatc agttcttcgt tgctgaggtt ctcgttcggc
    10561 gcatagcccc agtagacttt acgcacacgc tcatggagat actccgcaaa ggcttcggct
    10621 aaacggtcgg caagcgcttt caccatgatt ttgttgtaat catcgtgctg cgcttcaagg
    10681 gcatcagcca gtgcgtcctc ttccagcccg ccagtcacgg caaatgcgcc gatgtaatct
    10741 gctttaccag aaagcttcgg cgcaacgaag tcagcgagac agtagttagc gaatcccgtt
    10801 ttttcggtct gttgacgcag atggtggctg acgttgatca catgggtgcg cgtttcgtca
    10861 cggtagattt caatgtcatc gcccacacgg tttgccggga acaggcccac cacgccacgc
    10921 ggattcagcg ttttctcggc gcttaattta tccagcatgt cgttggcgtc tttaaacagc
    10981 cgctgcgcct caacgcccac cacttcatct tccagaatgc gaggatactt cccggccagc
    11041 gaccaggtca taaagaacgg tgtccagtcg atgtaattac gcagcgtttc gatgctggct
    11101 tccacttcct gcacgccgag acggtgcgcc accggcggcg tgtaagcctg ccagtcaaat
    11161 gcgaaatcgt tatcgcgcgc cgcttccagc gtgaccggtg gtgtgcgtgg tttcttgcgc
    11221 ccgtgctgaa tacgcacggt ttcgtactcc ttgcaggtac gagcgacaaa atcatcacgc
    11281 tgagtatcgg aaagcagcgc cgctaccaca ccaacggtgc gcgaggcatt ctgcacatac
    11341 accgtagggc cgctgtagtt ctgctcgatt ttcaccgccg tgtgcgcttt tgaggtggtc
    11401 gcgccgccaa tcagtaacgg aatggcgaag ccctgacgct ccatctcttt cgccacgtta
    11461 accatctcgt ccagcgacgg cgtgataagc cccgaaaggc caatcaaatc agcattcact
    11521 tctttggcga tacggagaat tttttccgca ggcaccataa cgccgagatc gacaatttcg
    11581 tagttgttac attgcagcac cacaccaacg atatttttac cgatgtcgtg gacatcgcct
    11641 ttcacggtgg cgatcaccat cttgccgttg gttttaccct gctctttgct ggcttcaata
    11701 aacggttcga gatacgccac tgcctgtttc atgacgcgcg ccgatttgac cacctgtggc
    11761 aggaacattt tgccttcgcc aaacagatcg ccgaccacgt tcatgccgtc catcaacggg
    11821 ccttcaatca cttcaatcgg gcgcgtagcc tgctggcggg cttcttcagt atcctgctcg
    11881 ataaactcgg ttatgccttt gaccagcgag tattccagac gtttattcac gtcccacgag
    11941 cgccactccg cttgctgggc gttagcagtg tcgtccgctt tactgccgcg atatttctcg
    12001 gcaagctcca gtaaacgctc agtgccatcg tcacgacgat taagaatcac atcttccacc
    12061 gcgtcgcgca gttcagcggg taggtcgtcg taaatcgcca gttgcccggc gttgacgatc
    12121 cccatatcca tgccattgcg aatagcgtag tagaggaaca ctgcgtgaat ggcttcgcgc
    12181 accggatcgt tgccacggaa tgagaaagaa acgttagata cgccgccgga aatcagcgcg
    12241 tgcggcagtt cgcgtttgat gtcttcacac gcgccgataa agtcctgcgc gtagttgttg
    12301 tgctcttcaa taccggtggc gaccgcgaag atgttcgggt cgaagatgat atcttctggc
    12361 gggaagccaa cctcttcggt gaggattttg tacgcccgac ggcaaatctc gattttccgt
    12421 gcgcgagtat cggcctgtcc ctgttcgtca aaggccatca ccaccactgc cgcaccgtag
    12481 cgacgcaaca atttcgcgtg atggataaag gcgtcgacgc cctctttcat cgagatagag
    12541 ttaacaatgc ctttgccctg gatacacttc agaccttgtt caatgacatc ccattttgag
    12601 gagtcgatca taatcggcac gcgagcgata tccggttcac cggcaatcag attgagaaaa
    12661 cgcaccatcg ccgcttcggc atcgagcatc ccttcatcca tgttgatatc gataatctgc
    12721 gcgccgtttt ccacctgctg acgcgccaca tccagcgcct cgctgtattt ctcttctttg
    12781 atcaggcgct tgaacttagc agagccggtg acgttggtgc gttcacccac gttcacaaac
    12841 aggctatcgt cgccaatgtt cagcggctcc aggccagaca aacggcaggc tacgggaatt
    12901 tccggcagtt tgcgcggcgc taatccttct actgcacgac tcatcgctgc aatatgttgc
    12961 ggcgtggtcc cgcagcaacc accgacgata ttgagaaagc ccgcttgcgc ccattcacgt
    13021 atctgttttg ccatcgtgtc ggcgtcgaga tcgtattcac caaaggcgtt aggcagcccg
    13081 gcgttcgggt gggcggtgac gtagcattcc gcaatccgtg atagttcctg cacgtactgg
    13141 cgcagttcat cgggccccag cgcacagttc aggccaaagg tcagcgcttc ggcgtggcgc
    13201 aatgagttgt aaaatgcttc ggtggtctgc ccggagagcg tgcgcccgga ggcgtcggtg
    13261 atggtgccgg agatcataat cggcagctca acgcccagcg cttcaaactc cgttttcacc
    13321 gcaaagaccg ccgctttggc gttaagggtg tcgaaaacgg tttcaatcag gatcagatcc
    13381 gcaccacctt ccaccagcgc tttggtggat tcgcgatagg ccgccgccag ctggtcaaaa
    13441 gtgatgttgc ggaatgccgg atcgttgacg tccggagaaa tagacgccgt gcggttggtt
    13501 gggccaagaa caccggcaac gtagcgcggt ttctccggcg tgcgcgcggt ccactcgtca
    13561 gcacaggcgc gcgccagttt cgccgccgca aagttgattt ccgccgacag ggattccatc
    13621 tggtaatccg ccatcgcaat ggtcgtggag ttgaaggtgt tggtttcgat gatatccgcg
    13681 cccgcttcaa agtaggcgtt gtggatagcg gcgatcactt ccggtttact gagtaccagc
    13741 aggtcgttgt tgcctttgag gtcgcatggc cagtcggcaa agcgttcacc acgaaaatcg
    13801 gcttcgttca gtcgatagct ctggatcatg gtgcccatac cgccgtccaa caccagaata
    13861 cgttcattta actgcgcacg cagttgttcc actttgctgc tcacacttgc tcccgacacg
    13921 cttaacccag atttaataaa aattcaacaa accatactgg aataaacgca tctgtggtaa
    13981 aagcgaccac cacgcaacat gagatttgtt caacattaac tcatcggatc agttcagcaa
    14041 ctattgcatt agctaacaat aaaaatgaaa ataatttcca cgatacagaa aaaggaggct
    14101 gtcatggtcg cacccattcc cgcgaaacgc ggcagaaaac ccgccgttgc caccgcacca
    14161 gcgactggac aggttcagtc tttaacgcgt ggcctgaaat tactggagtg gattgccgaa
    14221 tccaatggca gcgtggcact cacagagctg gcgcaacaag ccgggttacc caattccacg
    14281 acccaccgcc tgctaaccac gatgcaacag cagggtttcg tgcgtcaggt cggcgaactg
    14341 ggacactggg caatcggcgc acatgccttt atggtcggca gcagctttct ccagagccgt
    14401 aatttgttag caattgttca ccctatcctg cgcaatctaa tggaagagtc tggcgaaacg
    14461 gtcaatatgg cggtgcttga tcaaagcgat cacgaagcga ttattatcga ccaggtacag
    14521 tgtacccacc tgatgcgaat gtccgcgcct atcggcggta aattgccgat gcacgcttcc
    14581 ggtgcgggta aagccttttt agcccaactg agcgaagaac aggtgacgaa gctgctgcac
    14641 cgcaaagggt tacatgccta tacccacgca actctggtgt ctcctgtgca tttaaaagaa
    14701 gatctcgccc aaacgcgcaa acggggttat tcatttgacg atgaggaaca tgcgctgggg
    14761 ctacgttgcc ttgcagcgtg tattttcgat gagcaccgcg aaccgtttgc cgcaatttct
    14821 atttccggac cgatttcacg tattaccgat gatcgcgtga ccgagtttgg cgcgatggtg
    14881 attaaagcgg cgaaggaagt gacgctggcg tacggtggaa tgcgctgact ttttctggcg
    14941 ggcagaggca atattctgcc catcatacct gagtggcaat agaataaggg tgtctgttaa
    15001 tcgcattgac gccaaaataa cttaatgtca tacacttcac acacttttat taactcagca
    15061 ttatttttaa acatcaaacc acttaattat taacaaaatc gcaagatctc attccaccga
    15121 aacttttacg gctgcattat gagattgagc gatttatgat atttttttag ttgtaatcat
    15181 attaaatttt ttcactgttc tgatagttaa aattcaagac atcaataaac aatgagatat
    15241 ttaaatgatt actcgtattc ctcgtagttc tttctctgca aatattaata atacagccca
    15301 gacaaatgaa caccaaaccc tgagtgaatt gttttataaa gaactcgagg ataaattttc
    15361 tggcaaggag ctggcgacgc ctctattaaa aagcttctca gagaactgta gacaaaatgt
    15421 tcgtcatatc tttagcaaca aggattttgt cattaaattt tccacgtccg tcttacaagc
    15481 tgataagaaa gaaattacga tgattaataa aaacgaaaac acgacactca ctcaaaccat
    15541 tgctacaata tttgaagaat acctaatgga aattttacct caacgctcag acactcttga
    15601 taaacaagaa ttaaaccaaa aatcagatag aaaagaaaaa gaattcccaa gaattaaact
    15661 taatggtcaa tgttatttcc cggggcgccc caaaaccgta tagtataccg acacattgct
    15721 gcacaatata ttaatgatat ttatcagaat gttgattaca aaccccatca agatgattac
    15781 tcttcagctg aaaaatttct cactcacttc aacaaaaaat gcaaaaacca gactttggcg
    15841 ttgatttcca gccgtcctga ggggcgttgc gttgctgcct gcggtgattt cgggctagtt
    15901 atgaaagcat attttgacaa gatggaatca aatggcatca gtgttatggc agccatatta
    15961 ctggtggata accatgcttt gacggtccgg ctaagaataa agaacacaac tgaaggatgt
    16021 acccattacg tggtttcggt ttatgatcct aatgtaacta acgataaaat aagaattatg
    16081 agcgaaagca aagaggatat taaacactat tctctgatgg attttatgaa tgtagattat
    16141 agcctcctga aatggtcaaa tgatcatgtt attaaccaat ctgttgcaat aattccagca
    16201 cttccgaaag aacagctatt gatgttaaaa ggaactgtgg atgaaataac ccctccatta
    16261 tcaccagcaa cgatgaattt gctaatggca attggtcaga atcaccaact tacgcaactg
    16321 atgattcagc tccagaaaat gccagaacta catagaacag aaatgttgac tgcctataat
    16381 agtattaacc tccccggttt atatttggct ataaattatg gcaatgcgga tatcgttgag
    16441 actattttca attcattgtc agaaacagga tatgaagggt tactctcgaa aaaaaatctc
    16501 atgcatattc tggaggcaaa agataaaaat ggtttttctg gattattttt agcgatatca
    16561 cgtaaggata aaaatgttgt aacctcgatt ctgaacgtct tacctaaact ggccgcaaca
    16621 catcatttag ataatgaaca ggtgtataaa ttcctgagtg ccaaaaatag aacgtccagc
    16681 catgttttat accatgttat ggcgaatggt gatgccgaca tgctgaaaat ttttttggtc
    16741 gcgttacctt tgttaattcg cacatgtcat ttgactaaag aacaggtact cgatctcctg
    16801 aaggcaaagg atttttatgg ttgcccaaga ctatacctgg cgatgcaaaa tggacatagc
    16861 gatatcgtga aagttattct cgaagcattg ccctgtctgg cccaggaaat taacatttca
    16921 gcttccgata ttgtagatct tctgaccgct aaaagtcttg cgcgcgacac gggtttgttt
    16981 atggccatgc agcgcggaca tatgaacgtt attaatacta tttttaacgc attacccact
    17041 ctttttaata cgtttaaatt cgataaaaaa atatgaagcc cctcctcctg gcaaataatt
    17101 ctaatgaata tcccggtttg ttttcagcga tacagcataa acaacaaaac gttgtagaga
    17161 cggtttatct tgctttatct gaccatgcac gcctgtttgg atttaccgct gaagatatta
    17221 tggatttttg gcaacacaaa gcgccacaaa aatactctgc ctttgagttg gcttttgaat
    17281 ttggtcaccg ggttattgct gaattaatcc ttaatacatt aaataagatg gctgaaagct
    17341 ttggctttac ggataaccct cgatacattg cggagaaaaa ttatatggaa gctttactca
    17401 aaaaagcatc tccccatacc gtacgctaaa tctttgcctg cgccgatacg cataaacatc
    17461 ttccacatgc ccttcacgta tgcggttttg tagtgcgcgc cagtaatcag cgcggaacag
    17521 gtcggcgtgc atctcttcaa acagcggacc aatacgcggg tcggcgcata gccagtggcg
    17581 aaactcttcc gggaaaacat cgcccggcga gacgctgtac cacggttcgc tggcaagttc
    17641 gtcttccgga tagcgcggcg gcgggatgtc gcggaaattc acttccgtca tgtagcaaat
    17701 ttcatcgtaa tcataaaaaa ccacacgccc gtgacgggtg acaccgaagt ttttaaacag
    17761 catgtcgcca gggaaaatgt tagcagcggc aagctggcga atagcgttac cgtattcttc
    17821 aatggcgtcg cgcaactgct gaccttccac ttgttccagc cagatattga gcggcaccat
    17881 ccgccgctcg attatagaga tggcgaatta caatctgctc accaagatcg gtgatttttt
    17941 ccgctgcttc ctggagcagt aattccatta ataccgggga aatatgccgc ttctccagca
    18001 caaagttttc aaactcctgg gtgtccgcca ttcggcccac acggtcgtgc tctttcacca
    18061 gttgatagca ggcacgaacg tgagcggcag acatctcttt ctgcggcgcg aacttgtctt
    18121 tgatgacttt gaatacccga tcaaagcccg gcagcgtaaa caccaacatc accattccac
    18181 gaatacccgg cgcttcaatg aactgctcat tacagccctg tagataaacg agatattcgc
    18241 ggtagctttc ggttttggcg tgcttctggc agccgatagc catatacaat tcagcagtgg
    18301 ttttacctgg cagaatttcc cgtagccact cgaccagcgc tgcgggcagc ggtgcataaa
    18361 ccataaaata agaacgcgca aagccaaaaa caatgctcgc ttcggcggtc gtcgtcaggc
    18421 aggtatcaat aaataactcg ccgtcgtccg tctggtggat cggcagtaag aagggcaacg
    18481 tgccggaagg cgtgatcagt ttgcccacca gccaggcggc tttattgcgg taaaacagtt
    18541 cgttcgccac ctgtaaatga ctttccgcga ggttgtctgt ccccagcgtt tccgtcagat
    18601 ggcgaatgat gtaatgaatg tcacggcttt tattctgcca gcgcaggcgc agcggcaggt
    18661 cgctgataac gcgcatcagt agagattccc agccgtgatc ggggtgaaag tctttcgcca
    18721 gcgggcgggg aatggtacga aagcggcgct ctggctgaga gctaaagata aaaagccgct
    18781 cgggagtaag cgagcggtgg tcaaataacc gacagtacac ggagttaaaa aagctctccg
    18841 caatctcgaa gcgcgggtaa tccggcaaca gccgcgtgta atgctcttta acgcgcagta
    18901 aaaatgccgc gtccgtactt tggccgttag taatgcagcg cagttgctcc acgaccagac
    18961 caacgtgatg atcgtaaaga tggatacggt ttttcatcgc ctgctggaca gcatgccagt
    19021 cggcctgttc gaaacgctgc tgcgccccgg aagtcacttc gaggaatcga ccatactgag
    19081 cgtcgaagcc ttgcaaaatg gtttgagcaa tcagtaattc caggccacgc ggcatttagc
    19141 gccctcatca ggagcagaga attgccggat gcggcgtaaa cgccttatcc agcctacgtt
    19201 cggcaacggt tgtaggcatg ataagacgcg caagcgtcgc atcaggcatc gtgcaccgat
    19261 tgccggatgc ggcgtaaatg ccttatccgg cctacagtca gcaacgtttg taggttgctt
    19321 agaactgcga ttcttcagtg gagccagtca gcgcggtgac tgaagacgtg ccgccctgaa
    19381 taatagtcgt cactttatcg aagtaacctg tacccacttc ctgctggtga gatacgaagg
    19441 tatagccatc tttcgcggcg gcaaattccg gctgctgcac tttctcaaca tagtgcttca
    19501 taccttcgcc ctgcgcataa gcgtttgcca ggtcgaacat gttaaaccac atgctgtgga
    19561 tacctgctag ggtgatgaac tggaacttgt agcccatatc cgacagctgc tgctggaagc
    19621 tggcaatagt tttgtcgtcg aggtttttct gccagttgaa cgacggcgag cagttataag
    19681 ccagcagttt gcccggatat ttcgcgtgga tagcttgtgc aaagcgacgc gccagttcca
    19741 gatccggcgt ggaggtttca caccagacca ggtcagcata tggcgcatac gccaggccac
    19801 ggctgatcgc ttgctcaatg cccgcatgag tacggaagaa gccttcactg gtacgctcgc
    19861 cggtaataaa ttcgctgtca tacgggtcgc aatctgaggt gatcagatcc gccgcatcag
    19921 catcggtacg tgcaaccagc agggttggaa cgcccgtcac gtcagctgcc agacgcgccg
    19981 cgaccagttt ctgaatagct tcctgagttg gcactaaaac tttgccgccc atgtgaccgc
    20041 atttcttcac tgacgccagc tgatcttcga agtgaactgc cgcagcaccg gcttcaatca
    20101 tcgctttcat cagttcaaag gcattcagga caccgccaaa accggcttcc gcatcggcaa
    20161 cgatcggcag gaagtaatcg acatagcgcg gatcgcccgg ctcaatgccc acggaccatt
    20221 ggatctgatc ggcacgacgg aaggtgttgt tgatccgctc caccacagct ggcaccgagt
    20281 ttgccggata gagcgactga tccggataca tgctggccgc caggttagcg tccgccgcta
    20341 cctgccatcc cgacagatag actgcttcaa tacccgcttt cgcctgttgc agcgcctgac
    20401 cgccagtcag tgcaccgagg ttgttgatgt agcctttttt cgactcaccg tgcagcagac
    20461 gccacatttt cgctgcgccc agttgcgcca gcgtgcattc aggattgact gaaccgcgta
    20521 atttcaccac atcttccgca ctgtatgggc gagtaatgcc ttcccaacgc ggttgagtcc
    20581 actctttctg taattcttca atttgttgtg tacgggtttt catgtgcaga tgctccatag
    20641 ttatgtggtg gtttacgcta acaggcggta gcctggcagg gtcaggaaat caattaactc
    20701 atcggaagtg gtgatctgtt ccatcaagcg tgcggcatcg tcaaaacggc cctgggagaa
    20761 acgttcttcg cccagttcgc tggcaatgac tttcatctct tcgcccagca tctggcggaa
    20821 caaggctttg gtcaccggtt tgccattgct caacgttttt tgatgatgga tccactgcca
    20881 gatcgaggta cgggaaattt cagccgtcgc cgcatcttcc atcaggccat aaatcggcac
    20941 gcagccgttg ccggagatcc acgcttcgat gtactgcaca gccacgcgaa tgttagcgcg
    21001 cataccttct tcggtgcgtt caccatcaca aggtgccagc agctgatcgg cagtaatcgg
    21061 cgcgtcttgt tcgcgcaata cttcaagctg atttttacgg gagccgagaa tgtcgttgaa
    21121 taccgccatt gccgtgtccg caaggcctgg gtgagcgatc catgtgccat cgtgaccgtt
    21181 attggcttcc agcgctttat ccgcttttac tttgttgagc acctggttat tacgctcttc
    21241 atctttgctc ggaataaacg cggccatgcc gcccatcgca aaagcaccgc gtttatggca
    21301 ggttttaatc agcaggcgtg agtaagcatt caggaatggt ttatccatcg tcacggcctg
    21361 tctgtctggc aggacacgat cgggatagtt tttcaacgtt ttgatatagc tgaagatgta
    21421 atcccagcga ccgcagttca gaccaacaat atggtcgcgc agcgcgtgaa ggatttcgtc
    21481 catctggaac acggcgggca gcgtttcaat cagcaacgtc gccttgatgg tgccgcgcgg
    21541 cagattaaag cgatcttctg catagctgaa gacttcgctc caccaggccg cttcctgcta
    21601 ggactgggtt ttcggcagat agaaataggg accgctgccc tttgccaaca gtgcctgata
    21661 gttgtggaag aaatagagcg caaaatcaaa caggctaccg gggattgcct caccacgcca
    21721 ggtgacatgt ttttccggca agtgcagacc gcgtacccga caaatcaaaa ccgctggatt
    21781 gggcttgagc tgataaattt tgcctgcttc attggtatag ctgatggtac cgttaaccgc
    21841 atcgcgcagg ttaatttgcc cgtcgatcac tttgttccag tctggtgcca gtgaatcttc
    21901 gaaatcggcc ataaagactt tcacattggc gttcagtgcg ttgatcacca tcttgcgctc
    21961 taccgggcca gttatctcta cgcggcggtc ttctaagtcc gcaggaatcc cgcgaatttt
    22021 ccaatcagca tcgcgaatgg aagccgtttc cgaaataaaa tcaggcaacg ttccgttatc
    22081 aatatcttgc tgctgctgaa tgcgcgctgc cagaagttta ttgcgttgtg gcgtaaaatg
    22141 cgtcaccagc tcagtcagaa attctaccgc ttcggcagta agaatttgct tctcctgctc
    22201 gccatacggc cttgtgaaag ccagttcatc ggttgttgtt gcctgttcag tcatcgtgca
    22261 gctcctcgtc atggatccga aaacttcccc tctgtgaacg aaggaacgtt gtgcgctttt
    22321 cgttcagcac aactaagact actcatttaa aatgcaaaat caaaaacaat ttccatttta
    22381 attaaaaata cttgataact tattgataac aatatgaata aaatttattc acttgctgag
    22441 gtgcgtttcg gaaataaaaa aggcacccga aggtgcctga ggtaaggtgc tgaatcgctt
    22501 aacgatcgac tatcacagaa gattaatcca gcgttggatt catgtgccgt agatcgtatg
    22561 gcgtgatctg atagacgtaa tagttgagcc agttggtaaa cagcaaatta ccgtgactac
    22621 gccagctcgc tcgcggtttg ttttgcggat cgttgtgcgg gaaatagtta tacggtacat
    22681 ccgggtctag tccggcttcc acatcgcgta aatattcctg cgccagcgtt tgcgcatcat
    22741 attcgggatg gcccgtcaca aaggcaatgc gcttatcttt gctggcaaac agatatgcat
    22801 ccccttcttc cgtctctgcc agaatttcca gatcggtgta atcacgaatc aacgctgccg
    22861 gaaagtcagc atagcgcgaa tgcggtgcca ggaatgaatc atcaaagcca cgcgtcagaa
    22921 gcgcatgagg atggagaata tgatgctcgt aaacgccaga gagtttgtcg gtgcgagttt
    22981 gcttaggaat gccgtagagg atattgagcg cggcctgtac cgcccagcag acaaacagcg
    23041 tcgaggtgac gtgatctttc gaccactcca gcacctgttt gatctgcggc cagtaagcga
    23101 catcattaaa ctccaccagg cccagcggcg caccggttac aatcagaccg tcaaagttct
    23161 gttcctgaat atcttcaaag ttacagtaga agttgttcag atgctctgcg ggcgtgttgc
    23221 gcgattcacg ggaatcgatg cgcaacagct gaatatcgac ctgcaaaggt gagtttgaaa
    23281 gcaggcgcag aaactgattt tcagtttcaa tcttcttcgg catcaggtta aggatcagaa
    23341 ccttaagtgg acgaatttcc tgaccagacg cacgagaagt tgtcatcaca aagacgtttt
    23401 cttcacgcaa gaaattgacg gcgggtagct cgtccggcac acgaatcggc ataacctgat
    23461 tacctcacta catacgctta tacgtttaga catccagata gctgaagata accagaaaat
    23521 ttgccaatgt cgagcctgca tgttgaaggt gagaaagttt caaggaaaag gctacgttag
    23581 aatataagaa tgacgaaaag gagagaaaat ggttattagt attcgccgct cacggcatga
    23641 ggaaggggag gaactcgttg cgatttggtg tcgatctgtc gatgccaccc acgatttttt
    23701 atcagcagag tatcgggccg agctggaaga gctggtgcgt tcctttctgc cggaagcacc
    23761 gttgtgggtc gcggttaatg aacgggagca gccggttggg tttatgttgt taagtgggca
    23821 gcatatggat gcgctgttta tcgatcctga tgtgtgcggc tgcggcgtgg gtcggatgct
    23881 ggtggagcat gcgctctcga tggcaccgga actgacaacc aacgttaatg agcaaaatga
    23941 gcaggcggtt gggttctata agaaggtggg ttttaaggtt acgggacgct ctgaggtgga
    24001 cgatttgggg aaaccgtatc cgttgctgaa tctggcgtat gtgggggaat aagccgcctc
    24061 tgccgtgcct gcccaagctg gagaagacg
//
LOCUS       DAJTSI010000056        23119 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000056 DAJTSI010000000
VERSION     DAJTSI010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23119
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.056"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(49..711)
                     /gene="fsa"
                     /locus_tag="OO327_001965"
     CDS             complement(49..711)
                     /gene="fsa"
                     /locus_tag="OO327_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR6018513.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            842..1741
                     /locus_tag="OO327_001966"
     CDS             842..1741
                     /locus_tag="OO327_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR6018514.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            1747..4179
                     /locus_tag="OO327_001967"
     CDS             1747..4179
                     /locus_tag="OO327_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR6018515.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            4325..5140
                     /gene="ybiV"
                     /locus_tag="OO327_001968"
     CDS             4325..5140
                     /gene="ybiV"
                     /locus_tag="OO327_001968"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR6018516.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            5292..6557
                     /locus_tag="OO327_001969"
     CDS             5292..6557
                     /locus_tag="OO327_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR6018517.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(6798..8390)
                     /locus_tag="OO327_001970"
     CDS             complement(6798..8390)
                     /locus_tag="OO327_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR6018518.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            8609..9529
                     /gene="ldtB"
                     /locus_tag="OO327_001971"
     CDS             8609..9529
                     /gene="ldtB"
                     /locus_tag="OO327_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6018519.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(9587..10705)
                     /locus_tag="OO327_001972"
     CDS             complement(9587..10705)
                     /locus_tag="OO327_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR6018520.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(10702..11169)
                     /gene="mntR"
                     /locus_tag="OO327_001973"
     CDS             complement(10702..11169)
                     /gene="mntR"
                     /locus_tag="OO327_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR6018521.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            11355..11483
                     /gene="mntS"
                     /locus_tag="OO327_001974"
     CDS             11355..11483
                     /gene="mntS"
                     /locus_tag="OO327_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR6018522.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            11755..13338
                     /gene="opgE"
                     /locus_tag="OO327_001975"
     CDS             11755..13338
                     /gene="opgE"
                     /locus_tag="OO327_001975"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR6018523.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(13386..13901)
                     /gene="ompX"
                     /locus_tag="OO327_001976"
     CDS             complement(13386..13901)
                     /gene="ompX"
                     /locus_tag="OO327_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR6018524.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(13954..14019)
                     /locus_tag="OO327_001977"
     CDS             complement(13954..14019)
                     /locus_tag="OO327_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018525.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            14254..15141
                     /gene="rhtA"
                     /locus_tag="OO327_001978"
     CDS             14254..15141
                     /gene="rhtA"
                     /locus_tag="OO327_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR6018526.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            15440..15943
                     /gene="dps"
                     /locus_tag="OO327_001979"
                     /gene_synonym="pexB"
     CDS             15440..15943
                     /gene="dps"
                     /locus_tag="OO327_001979"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR6018527.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            16347..17093
                     /gene="glnH"
                     /locus_tag="OO327_001980"
     CDS             16347..17093
                     /gene="glnH"
                     /locus_tag="OO327_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR6018528.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            17232..17891
                     /gene="glnP"
                     /locus_tag="OO327_001981"
     CDS             17232..17891
                     /gene="glnP"
                     /locus_tag="OO327_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR6018529.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            17888..18610
                     /gene="glnQ"
                     /locus_tag="OO327_001982"
     CDS             17888..18610
                     /gene="glnQ"
                     /locus_tag="OO327_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR6018530.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            18871..21095
                     /gene="ybiO"
                     /locus_tag="OO327_001983"
                     /pseudo
     CDS             18871..21095
                     /gene="ybiO"
                     /locus_tag="OO327_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(21092..22018)
                     /gene="rlmF"
                     /locus_tag="OO327_001984"
     CDS             complement(21092..22018)
                     /gene="rlmF"
                     /locus_tag="OO327_001984"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR6018531.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            22294..22554
                     /locus_tag="OO327_001985"
     CDS             22294..22554
                     /locus_tag="OO327_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6018532.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            22819..>23119
                     /locus_tag="OO327_001986"
     CDS             22819..>23119
                     /locus_tag="OO327_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR6018533.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT         5665 a         6071 c         5743 g         5640 t
ORIGIN      
        1 tgcactccgc cacgtcatca gcaatttatg aggatgcaga acgtccggtt aaatcgacgt
       61 tctgccaaac gctccctgcc agtcctgctc aaacttcgtc acagcggctt ccaccgccgg
      121 atagctaatc atctgttgtg ccacatccag tggcagagta attgattcac atccggccag
      181 taagcagtcc agcgcctgac gcggggtttt gaaactcgct gccagcactt tcgcctgcgg
      241 cgcatgcatt ttcaataact ggtgtaagtc ggtcacagtc tgaatgccgc taccgccctg
      301 agcatcaata cgattaacgt aaggcgcaac atattccgca cctgccagcg ccgacagcag
      361 cccttgtgcc gcgccatata ccgcggttcc cagcgtcgga atcccttccg cttttaacat
      421 cttaatcgct gccagcccct cggctgtcac cggaactttc accacgatat ccgcaataat
      481 agaacgcagc ttaagcgcgt cattcaccat cccttcagca gtggtagcca ttacctgggc
      541 aaacagacgc ccctgaccgc ccatcgcttc atgaagttgc ggaagcacaa catccagcgg
      601 ttttttaccc gcggcgataa tgcttgggtt agtggtcaca cccgccagcg gaaaaatacg
      661 tgacagcgcc ttcaccgcaa caacgtctga agtatccaga tacagttcca taaccatcct
      721 caaaatgtac tttttgtctg aatgtagact atcacgggaa aactctctga acagttgacg
      781 cacatcaatc taactttcat tcgaaagtat tttaatgttt gaacgaaaca gagaggcaac
      841 tatgattttc aatattcagc gctactcgac ccatgatggc cccggtatcc gcacggtcgt
      901 atttcttaaa ggctgttcgc tgggctgccg ctggtgtcag aacccggaaa gccgcgcccg
      961 tacgcaggat ctgctgtatg acgcacgact gtgtctggaa ggctgcgagc tgtgcgctaa
     1021 ggccgcgccg gaagtgattg agcgcgcgct gaatggtttg cttattcatc gggaaaagtt
     1081 aaccccggag catctgacgg cgttaaccga ctgctgtccg acacaggcgt taaccgtgtg
     1141 tggtgaagtg aaaagcgttg aggagatcat ggcgaccgtt ctgcgcgata aaccgtttta
     1201 cgatcgcagc ggcggcggct taacgctttc gggtggtgaa cccttcatgc agccggaaat
     1261 ggcgacggcg ctgctgcaag cctgccacga ggcaggcatt catactgcgg tagaaacctg
     1321 tctgcatgtg ccgtggaaat atatcgcccc ttctctgccc tatatcgatc tgtttcttgc
     1381 cgatttaaaa cacgttgccg acgcgccgtt taaacagtgg accgacggta acgccgccag
     1441 ggtgctggat aacctgaaaa aactcgccgc agcgggcaaa aaaatcatta tccgcgtgcc
     1501 gctgattcag ggctttaatg ccgacgaaac ctctgtaaaa gccattaccg attttgccgc
     1561 cgacgagctg cacgttggcg aaattcattt tctgccctac cacacgctgg gcatcaacaa
     1621 atatcactta cttaatctgc cctatgacgc cccggaaaaa ccgcttgatg cgccagagct
     1681 gctcgatttt gcccagcagt atgcctgcca gaaagggtta accgcgacct tacgaggata
     1741 acaaccatga ccacactgaa actggacacg ctcagcgacc gcattaaagc gcacaaaaat
     1801 gcgctggtgc atattgtgaa gccgccagtc tgtaccgagc gcgcgcagca ctataccgag
     1861 atgtatcaac aacatctcga taagccgatc ccggtacgtc gcgcgctggc actggcgcat
     1921 cacctggcga atcgcaccat ctggatcaaa cacgatgagt tgatcattgg caaccaggca
     1981 agcgaagttc gcgccgcgcc gatcttcccg gaatatactg tctcgtggat cgaaaaagag
     2041 attgatgatc tggcagatcg tcccggtgct ggctttgcgg tgagcgaaga gaacaaacgt
     2101 gttctgcatg aagtgtgccc gtggtggcgc ggtcagacgg tacaggatcg ctgctatggc
     2161 atgtttaccg atgagcaaaa aggtctgctg gcgaccggca tcattaaagc ggaaggcaat
     2221 atgacctccg gcgatgcgca cctggcggtc aacttcccgc tgctgctgga aaaagggctt
     2281 gatggtctgc gtgagaaagt agcggaacgt cgctcgcgca tcaatctgac ggtgctggaa
     2341 gatttgcacg gcgaacagtt cctgaaagcg attgatatca tgctggtggc agtcagtgaa
     2401 cacattgaac gtttcgctgc cctggcgcgt gcaatggccg cgaccgaaac ccgcgaaagc
     2461 cgtcgcgatg aactgctggc gatggcagaa aactgcgatc ttatcgccca ccagccgccg
     2521 cagactttct ggcaggcgct gcaactgtgt tacttcatcc agttgatttt gcagattgaa
     2581 tctaacggtc actcggtgtc gtttggtcgt atggatcagt atctctaccc gtactatcgc
     2641 cgcgacgttg aactgaacca gacgctggat cgcgaacacg ccatcgaatt gctgcatagc
     2701 tgctggttga aattgctgga agtgaacaaa atccgctccg gctcccactc aaaagcctct
     2761 gcgggaagtc cgctgtatca gaacgtcacc attggcggac aaaatctggt tgatggtcaa
     2821 ccaatggacg cggtgaatcc actctcttac gcgatcctcg aatcctgcgg tcgcctgcgt
     2881 tccactcagc ctaacctcag cgtgcgttac catgcaggaa tgagtaacga tttcctcgac
     2941 gcctgcgtac atgtgatccg ctgcggcttc gggatgccgg cattcaacaa cgacgaaatc
     3001 gtgatcccgg aatttattaa actcggtatt gaaccgcagg acgcttatga ctacgcagcg
     3061 attggttgta tagaaaccgc cgtcggtggc aaatggggct atcgctgtac cggcatgagc
     3121 tttatcaact tcgcccgcgt gatgctggcg gcgctggaag gcgggcgtga tgccaccagc
     3181 ggcaaagtgt tcctgccaca agaaaaagcg ttgtcggcag gtaacttcaa caacttcgat
     3241 gaagtgatgg acgcgtggga tacgcaaatc cgttactaca cgcgtaaatc aatcgaaatc
     3301 gaatatgtcg tcgacaccat gctggaagag aacgtgcacg atattctctg ctcggcgctg
     3361 gtggatgact gtattgagcg agcgaaaagt atcaagcaag gcggcgcgaa atatgactgg
     3421 gtttctggcc tgcaggtcgg tattgccaac ctcggcaaca gcctggcggc agtgaagaaa
     3481 ctggtgtttg aacagggcgc gattggtcag caacagctgg ctgccgcact ggcggatgac
     3541 ttcgatggcc tgactcatga gcaactgcgt cagcggctga ttaacggtgc gccgaagtac
     3601 ggcaacgacg atgataccgt cgatacgctg ctggctcgcg cttatcagac ctatatcgac
     3661 gaactgaaac agtaccataa cccacgctac ggtcgtggtc cggttggcgg caactattac
     3721 gcgggtacgt cgtctatctc cgctaacgta ccgtttggcg cgcaaactat ggcaacgccg
     3781 gacgggcgta aagcccacac cccgctggca gaaggcgcta gtccggcctc tggtactgac
     3841 catctcggcc ctactgcggt cattggctca gtgggtaaac tgcctacggc agcgattctc
     3901 ggcggcgtgt tgctcaacca gaaactgaat ccggcaacgc tggagaacga atctgacaag
     3961 cagaaactga tgatcctgct gcgtaccttc tttgaagtgc ataaaggctg gcatattcag
     4021 tacaacatcg tttcccgcga aacgctgctg gaggcgaaaa aacatccgga tcagtaccgc
     4081 gatctggtag tgcgtgtcgc gggttattcc gccttcttca ccgcgctctc tccagacgct
     4141 caggacgata tcatcgcccg tactgaacat atgctgtaat ctcttcggcc cggctgcggc
     4201 gtcgccgggc taaatcattc acatcatcaa tttcatcctt actttcattc gaaatataat
     4261 ttgtgctctg cgtcacattg ttattacact gttacggttt cattttcgag ccaggagcac
     4321 acctatgagc gtaaaagtta tcgtcacaga tatggacggt acttttctta acgacgccaa
     4381 aacgtacaac caaccacgtt ttatggcgca atatcaggaa ctgaaaaagc gcggcattaa
     4441 gttcgttgtc gccagcggta atcagtatta ccagcttatt tcgttctttc ctgagctaaa
     4501 ggatgagatc tcttttgtcg cggaaaacgg cgcactggtt tacgaacatg gcaagcaact
     4561 gttccacggc gaactgaccc gacatgaatc gcggattgtt attggcgagt tgctaaaaga
     4621 taagcaactc aattttgtcg cctgcggtct gcaaagtgca tatgtcagcg aaaatgcccc
     4681 cgaagcattt gtcgcactga tggcaaaaca ctaccatcgc ctgaaacctg taaaagatta
     4741 tcaggagatt gacgacgtac tgttcaagtt ttcgctcaac ctgccggatg aacaaatccc
     4801 gttagtgatc gacaaactac acatagcgct ggatggcatt atgaaacccg tcaccagtgg
     4861 ttttggcttt atcgacctga ttattcccgg tctacataaa gcaaacggta tttcgcggtt
     4921 actgaaacgc tgggatctgt caccgcaaaa tgtggtagcg attggcgaca gcggtaacga
     4981 tgcggagatg ctgaaaatgg cgcgttattc ctttgcgatg ggcaatgctg cggaaaacat
     5041 taaacaaatc gcccgttacg ctaccgatga taataatcat gaaggcgcgc tgaatgtgat
     5101 tcaggctgtg ctggataaca catccccttt taacagctga cctctctacc ggagccatgc
     5161 gctccggttt tcccttcttt ttacatcctg tacatcacat caaatcatta aacttgcatt
     5221 aacttattta taacttgtat tgtcacatgc aaattagcat agtttcgaat gaaagttaat
     5281 gcaaggtggt tatggcttct acttttacca gcgacacatt gcctgccgat cacaaagcag
     5341 ctatccgtca gatgaagcac gcgctgcggg cgcagcttgg cgatgtccag cagatcttta
     5401 atcagctaag cgatgacatt gccacgcgag tggctgaaat caacgcactc aaagcacagg
     5461 gcgatgccgt ctggccggtg ctgtcttatg ccgatatcaa agcaggtcat gttactgcag
     5521 agcagcgcga acagattaaa cgtcgcggtt gtgcggtgat aaaaggccat ttcccccgcg
     5581 aacaagcgct aggctgggat cagtcgatgc tggactatct gaaccgcaac cgctttgacg
     5641 aggtctacaa aggccccggc gataatttct tcgggacgct cagcgcttca cgtcccgaga
     5701 tttaccccat ctactggtcg caggcgcaaa tgcaggcccg ccagagtgaa gaaatggcga
     5761 atgcgcagtc gtttctcaat cgtctgtgga catttgaaag tgatggaaag caatggttta
     5821 acccggatgt gagcgtcatc taccctgacc gtatccgccg ccttccgccc ggaacgacct
     5881 ccaaaggtct tggagcgcat accgactccg gggcgctgga acgctggctg cttccagcgt
     5941 atcagcacgt tttcgccaac gtctttaatg gcaatctggc gaaatacgat ccctggcatg
     6001 cggcacatcg tacggaagtt gaagagtaca cggtggacaa caccaccaaa tgttccgtgt
     6061 ttcggacatt ccagggctgg actgcgctct cggatatgct gcctggtcag gggttgctgc
     6121 acgtcgtgcc cattcctgaa gccatggcgt acgtactgtt acgtccgctg cttgatgatg
     6181 tgccggagga tgaactgtgc ggcgtagcgc ccggaagagt gttgccggta tcagagcaat
     6241 ggcatccact gttaattgag gcgttaacca gcattccaaa actcgaggcc ggagactccg
     6301 tctggtggca ctgcgacgtc atccattccg ttgcccccgt tgaaaatcaa cagggttggg
     6361 gcaacgtgat gtacattcct gcggcaccga tgtgcgagaa aaatcttgcc tacgcgcaca
     6421 aggtgaaggc cgcactggaa aaaggcgcat cgccgggcga cttcccgcgc gaggactatg
     6481 aaacaaactg ggaaggacgc tttacgcttg ccgacctcaa cattcacggt aagcgagcgt
     6541 tgggcatgga tgtttgagag ttaatggaag tgaattgatg tgggattaag aaaatcgatt
     6601 cgacgttact cagattgctg acaagtgcgc tttcttaatg cctgatgcgg cgttaacgcc
     6661 ttatccggcc tacaaagtcc tgcagattca atatattaca ggtactgcgt aggcctgata
     6721 agcgtagcgc atcaggctat tttgcatttg tcatcagccg tcgtcacccc gacagtatcg
     6781 gggtgacgga taatcactta ctcgatccct ttactacgca ggtaatcttc gtaattaccg
     6841 ctaaagtcga tcacgcgttc cggggtgatt tccagaatgc gggtcgccag ggagcttacg
     6901 aactcacggt cgtgtgaaac aaagatcagc gtgccctgat acagttccag cgccatgttc
     6961 agtgactcga tggattccat atccaggtgg ttggtcggtt cgtccatgat cagaatgttc
     7021 ggcttctgca tcattaactt accaaacagc atccgccctt tttccccacc ggaaagcact
     7081 ttagctggct ttttgatgtc gtcctggctg aacagcaaac gaccgagaat gctgcgtacc
     7141 gcctgctcgt catcgccttc ctgcttccac tgactcatcc attcgaacac ggtcagatca
     7201 ttttcaaact catattcgtg gtcctgggcg tagtaaccaa tgcgcgcgtt ttcagaccat
     7261 tttacggtgc cgctgtccgg ttgcagatcg cccaccagcg ttttcagcag cgttgattta
     7321 ccgacgccgt tggtacccag taccgcaagt ttttcaccga cctccagcag caggttgaga
     7381 tttttaaaca gtggaccgtt atcaaaccct ttggtcagac cttccacttc cagcgcgtta
     7441 cggaacagtt tcttatcctg ttcaaaacgg ataaacgggt tctgacggct ggaggctttc
     7501 acctcttcca gtttgatttt atcaatctgg cgcgcgcgcg aagttgcctg gcgagatttc
     7561 gaggcgttgg cgctaaagcg gctaacgaaa gattgcaact cagcaatctg cgctttcttc
     7621 ttggcgttat cggccagcag acgttcacgc gcttgggtcg ccgccgtcat gtactcatcg
     7681 tagttacccg gataaacgcg cagctcgccg taatccagat ccgccatgtg ggtacagacc
     7741 atgttaagga agtgacggtc gtgcgagatg atgatcatgg tgctgtcacg ctcgttcagc
     7801 acctgttcca gccagcgaat ggtgtcgatg tcgaggttgt tggtcggttc gtcgagcagg
     7861 agaatatccg gctcagcaaa cagcgcctgc gccagaagca cacgcagctt ccagccagga
     7921 gcaacttcac tcatcgggcc gtagtgctgt tccactggaa ttcccacgcc aagcaacagt
     7981 tcaccggcgc gagcttccgc agagtaaccg tccatttcgc cgtatttcac ttccagatcg
     8041 gccactttat agccgtcttc ttcactcatt tccggcaaag catagatgcg gtcgcgctcc
     8101 tgcttcactt cccacaactc tttatgcccc atgatcaccg tatccagcac agtgaactct
     8161 tcaaaggcaa actgatcctg acgcagctta ccaatgcgct cgttgggatc gagggaaacg
     8221 ttacccagcg tcggctcaag gtcgccgccg agaatcttca taaaggtgga tttaccacta
     8281 ccgttcgcgc caatcaggcc gtaacggttg ccgccgccga atttgacgga aatgttttca
     8341 aacaacggct tactgccgaa ctgcatggtg acgttactgg aaactaacac aggcgtatcc
     8401 tgaaaagaga tatgacaaac cgcgcattat gccataagtc tgaaagaaga tcctgctcct
     8461 cgcttaaggt ctacactcaa gccaaaggca aaaaatgtga tttcgtacac atctgatttc
     8521 actgtgagct ggaatgaact tataatgcgc ttccaatact ctaatattct caacccaatg
     8581 gcctgccagg cacaaaatct cgcttaacat gaatatgaaa ttgaaaacat tattcgcagc
     8641 ggccttcgct gttgtcggct tttgcagtac cgcctctgcg gtaacttatc ctctgccaac
     8701 cgacgggagt cgcctggttg gtcagaatca ggtgatcacc attcctgaag gtaacactca
     8761 gccgctggag tattttgccg cggagtacca gatggggctt tccaatatga tggaagcgaa
     8821 cccgggtgtg gataccttcc tgccgaaagg cggtactgta ctgaacattc cgcagcagct
     8881 gatcctgccg gataccgttc atgaaggcat cgtcattaac agtgcagaga tgcgtctgta
     8941 ttactatccg aaagggacca acaccgttat cgtgctgccg atcggcattg gtcagttagg
     9001 caaagatacg cctatcaact ggaccaccaa agttgagcat aagaaagcag gcccgacctg
     9061 gacgccgacc gccaaaatgc acgcagagta ccgcgctgcg ggcgaaccgc taccggctgt
     9121 cgttccggca ggtccggata acccgatggg tctgtatgca ctctacatcg gtcgcctgta
     9181 tgctatccat ggcaccaacg ccaacttcgg tatcggcctg cgtgtaagtc atggttgtgt
     9241 gcgtctgcgt aacgaagaca tcaaattcct gttcgagaaa gtaccggtcg gtacccgcgt
     9301 acagtttatt gatgagccgg taaaagcgac caccgagcca gacggcagcc gttatattga
     9361 agtccataac ccgctgtcta ccaccgaagc ccagtttgaa ggtcaggaaa tcgtgccaat
     9421 taccctgacg aagagcgtgc agacagtgac cggtcagcca gatgttgacc aggttgttct
     9481 tgatgaagcg atcaaaaatc gttccgggat gccggttcgc ctgaattaat cgtcaaaacg
     9541 taaaacaaag gcggactcat aatccgcctt ttttatttgc cagaccttac caggccggga
     9601 tcataactaa caaaacgtat cccaccagcg ccgcccacaa cagcatcggt attgaataga
     9661 gatggaaacg ccaccagatg cggcgatcgt tcgccatacg tagcgcaatc aaatttgcca
     9721 gcgatcctgg taataaacca aagcccccca cgtttaccgc ccatgccagt aataaagacg
     9781 gaggtacata attgagcagc aaaatggtac tcggcacgtt actgatcacc tgcgataaac
     9841 cgactgccgt taaccataac ccgggttcag ataaatgact cacgttaccc aacacgcctt
     9901 gcaacgctgg aagctgggtc agtaaatgga cgtcgataaa catcgccata aacaccagca
     9961 gcaacgtcca gtccacactc aacaccacgc gacgcgccag cagcacaaag cccgccgcca
    10021 caatcaccag tccccacagc tcttgtttga actccagcgc cgtcagaaag acgatataca
    10081 gccccagaca attccacacc agccgcggtt tccactccgg tgtttgcacc cccgtatggt
    10141 attgcagcgc ctttccaggg aaacaacacc agcacaacag caggagtgtc agcatcattg
    10201 cgccagccat cggtgccatt tgggcaataa atccggcaaa cgaaagacca gaacgtcccc
    10261 agataagaat attttgcggg ttgccaattg gcgtcagtag cgaaccagcg ttgactgcca
    10321 gcgcctcaaa aataatcagc cgattaaccg ggatctcaca cagtcttttt agcgtgatag
    10381 tcagcggaac aacaataaac agcgcgacat cgttggtcag aaaggtagaa agcagcgccg
    10441 ccgccagcac cataaacatc gccagccgac gctccgtagc aaagcggcgc accattttgc
    10501 gccccagcac atcaaaataa ccgcttaact ccacaccttt ggtcagcagc atcaggccgc
    10561 ttaaggtgat gatggtgtgc cagtcgatag cagcaggcca ggatttcggt gcaaagggca
    10621 cgaaaaagct taatccgata ccaacaagaa ttaataactg aaaaaaacga tcgccttgca
    10681 gcgtgcgtaa aaaaggcagg ctcatttggc accgtgtttc tgggtaaaca aacgaaaagc
    10741 gtcgagcgtc tcttcactaa catggtgctc catgccttcc gcgtcgcgac gggcgatttc
    10801 cggactgacg cccaacacca acaagaaatt ttcgactatc tgatgtcgct cgcggctttc
    10861 ctgcgccagc ttctctcctt ctgccgttaa aaacacgcca cgccagggga tcatttcaat
    10921 cagccccatg gttgccagcc gcttaagcat tttagccacc gtcggttgcg aaactcccag
    10981 acgagcagcc atgtccacct gacgagcttc ccccacttcc ctgatcaagt cagaaatcag
    11041 ctcaacgtaa tcatcaataa gctcgcgccg atgcgcctct ctgacctggc ggaacccttc
    11101 aacgtgctct tccacgttca ctaactgcgt cacttttttt gctattggcg tacctgcgcg
    11161 acgactcatt gtgcttcctc attatggtga cgcatacata gcgttctcat ttcgtgcgca
    11221 cattgtaaac cagagttgcg aaggtacaaa aaattaacgt tttagcaata gctatataat
    11281 atagcctgtg ctatatctgt atgtaatgca atcatccctc aaggagcgac gggattagca
    11341 agtcaggagg tcttatgaat gagttcaaga ggtgtatgcg cgtgtttagt cattctccct
    11401 ttaaagtacg gttaatgctg ctctctatgt tgtgcgatat ggtcaacaac aaaccgcagc
    11461 aagataaacc ttcagataaa tagcggcgtc gcggtacgcc gcttcactcc tgcttttatg
    11521 caggcataac gcgttttggt ctgaaaaaaa ccactttttg tcggatttgc aatccccttc
    11581 gcaaaagatt tgttcgtcag tagttgacct gaacggcggc tcgctctatc ttcttgcagc
    11641 cctgcgtata ttgcggctcg cggatgcgga cccctttccc ctcttcacgc actcttgcag
    11701 gtattgaccc ttgacgccag ggtaagcaca tggcgtttgt tacgatagtg gcatatgaat
    11761 ttaaccctca aagaatcgct tgttacccgt agccgggtat ttagcccgtg gactgcgttc
    11821 tactttttac agtcgctatt aattaacctc ggcttaggtt accccttcag tttgctctac
    11881 accgctgcgt ttacggctat tttgcttttg ctatggcgaa cattgcctcg cgtacaaaaa
    11941 gttctggtcg gtgtcagttc gctggtggcg gcttgttatt tcccttttgc tcaggcctac
    12001 ggcgcgccta atttcaatac attgctggca ttgcactcca ccaatatgga agagtcgacc
    12061 gaaatcctga cgatttttcc gtggtacagc tacctggtcg gcttatttat ttttgcgctc
    12121 ggcgtaatag caatcaggcg aaaaaaagag aatgaaaaag cgcgctggaa taccttcgac
    12181 agcctgtgtc tggtattcag tgtggcgaca ttttttgttg ctcccgtgca aaacctggcc
    12241 tggggtggcg tatttaaact gaaagatact ggctatccgg tatttcgttt tgctaaggat
    12301 gtcatcgtca ataataacga ggtgattgaa gagcaagaac ggatggcaaa actttccgga
    12361 atgaaagata cctggacggt cactgccgtt aagccgaagt atcagaccta tgtggtagtg
    12421 atcggtgaaa gcgcgcgtcg cgatgccctc ggtgcctttg gcggtcactg ggacaatacc
    12481 ccgtttgcca gcagcgttaa cggtttgata tttgctgact acattgctgc cagtggctcc
    12541 acgcagaaat cgcttggctt aacgctcaat cgcgtagtcg atggcaaacc acagtttcag
    12601 gataactttg tcaccctggc aaatcgcgcg ggcttccaga cctggtggtt ttccaaccag
    12661 ggccaaatcg gcgaatacga tactgctatc gccagcatcg ccaaacgtgc agatgaagtg
    12721 tacttcctga aagaaggtaa ttttgaagca gataaaaaca cgaaagacga agcgttactg
    12781 gatatgaccg ctcaagtgct ggcgcaagag cactcgcaac cgcagctgat tgttctgcat
    12841 ctgatgggct cgcatccgca ggcctgcgac aggacacaag gcaaatacga aacctttgtg
    12901 caatcgaaag aaacgtcgtg ctatctctat accatgacgc aaacggacga tttactgcgc
    12961 aagctgtacg atcagttacg caacagcggc agcagcttct ctctggttta cttttctgac
    13021 cacggtctgg cctttaaaga gcgcggcaaa gacgtgcaat accttgccca tgatgataaa
    13081 tatcagcaaa atttccaggt gccttttatg gtcatttcca gcggcgataa agcgcatcgg
    13141 gtgattaaag cccgccgctc agccaatgac ttcttaggct ttttctcgca gtggacggga
    13201 attaaagcga aggaaataaa tatcaaatac ccgtttatat ctgagaagaa agccgggccg
    13261 atatacatca ccaacttcca gttacagaag gtggattaca accatctcgg aaccgatatt
    13321 ttcgacccga aaccttaaaa acaaaaatcc gccccgagag gcggattttt atatcaccaa
    13381 agtgattaga agcggtaacc aacaccggca atccaggtgc ctacgtcaac gctacgaata
    13441 cggctctgct cgtaagagaa gtccagagca acgttttcca tcgggttgaa ctgcagacca
    13501 gcaccgtagg agaaaccgta gtcgctggtg tcgtgtttgt aggtcgggta ttcagtggtc
    13561 tggaatttac cataacccac acccactaca ccgtagatgc ttgcccagtc gttaatgcgg
    13621 taagccggac cagcagtgat gccgtagtac tggtttttgt tgtagtcacc agagcttgca
    13681 gtacggcttt tctcggtgta agtgaaagaa ccgatcacac ccagcgggct gttgtcttct
    13741 tcatagcggt atttcaggtt gaaaccgccc attttgttca tttggccctg agcgtcgctc
    13801 tgtgcgtaac cgccagttac ggtagaagtc gcagctacgg aagtacctgc ggtgaaagcc
    13861 agaactgcgg ccagtgctga aagacatgca atttttttca taaccacctc aaatgtgatt
    13921 caaataagtc ctaagtttta aatatatcaa aaattaatgg gaaactcttc gcgatttgtg
    13981 atgtctaacg ggccatttca tgtaacagaa cgtttccata caccgctatc catctaaatt
    14041 taaatcactt tttcagagaa ctgcgtaagt attacgcatg ttttccctgt cattcatcca
    14101 gattattcct aatcaccaga ctaatgattc catcaatcct ggcgcatttt agtcaaaacg
    14161 ggggaaaatt ttttcaacaa atgctcaacc agcattgggt atatccagta cactccacgc
    14221 tttacttaag tctagatatt tgtgggagaa aggatgcctg gttcattacg taaaatgccg
    14281 gtctggttac caatagtcat attgctcgtt gccatggcgt ctattcaggg tggagcctcg
    14341 ttagctaagt cactttttcc tctggtgagc gcaccgggtg tcactgcgct gcgtctggca
    14401 ttaggaacgc tgatcctcat cgcgttcttt aagccatggc gactgcgctt tgccaaagag
    14461 caacggttac cgctgttgtt ttacggcgtt tcgctgggtg ggatgaatta tcttttttat
    14521 ctttctattc agacagtacc gctgggtatt gcggtggcgc tggagttcac cggaccactg
    14581 gcggtggcgc tgttctcttc tcgtcgcccg gtagatttcg tctgggttgt gctggcggtt
    14641 cttggtctgt ggttcctgct accgctgggg caagacgttt cccatgtcga tttaaccggc
    14701 tgtgcgctgg cactgggggc cggggcttgt tgggctattt acattttaag tgggcaacgc
    14761 gcaggagcgg aacatggccc tgcgacggta gcaattggtt cgttgattgc agcgttaatt
    14821 ttcgtgccaa ttggagcgct tcaggctggt gaagcactct ggcactggtc ggttattcca
    14881 ttgggtctgg ctgtcgttat tctctcgacc gctctgcctt attcgctgga aatgattgcc
    14941 ctcacccgtt tgccaacacg gacatttggt acgctgatga gcatggaacc ggcgctggct
    15001 gccgtttccg ggatgatttt cctcggagaa acactgacac ccatacagct gctggcgctc
    15061 ggcgctatca tcgccgcttc aatggggtct acgctgacag tacgcaaaga gagcaaaata
    15121 aaagaattag acattaatta aatttacatt tctgtatggt tatgcataac catgcagaat
    15181 ttctcgctac ttttcctcta caccgtattt atatatcgaa ttatgcaaaa gcatatttat
    15241 tccgaaaatt cctggcgagc agataaataa gaattgttct tatcaatata tctaactcat
    15301 tgaatcttta ttagttttgt ttttcatgct tgttaccact attagtgtga taggaacagc
    15361 cagaatagcg gaacacatag ccggtgctat acttagtctc gttaattact gggacataac
    15421 atcaagagga tatgaaatta tgagtaccgc taaattagtt aaatcaaaag cgaccaatct
    15481 gctttatacc cgcaacgatg tctccgacag cgagaaaaaa gcaacagtag agttgctgaa
    15541 tcgccaggtt atccagttta ttgatctttc tttgattacc aaacaagcgc actggaacat
    15601 gcgcggcgct aacttcattg ccgtacatga aatgctggat ggcttccgca ccgcactgat
    15661 cgatcatctg gataccatgg cagaacgtgc cgtgcagctg ggcggtgtag ctctggggac
    15721 cactcaagtt atcaacagca aaaccccgct gaaaagttac ccgctggaca tccacaacgt
    15781 tcaggatcac ctgaaagaac tggctgaccg ttacgcaatc gtcgctaatg acgtacgcaa
    15841 agcgattggc gaagcgaaag atgacgacac cgcagatatc ctgaccgccg cgtctcgcga
    15901 cctggataaa ttcctgtggt ttatcgagtc taacatcgaa taaatccatc gctgatggtg
    15961 cagaacttta gtacccgata aaagcggctt cctgacagga ggccgttttg ttttgcagcc
    16021 cacctcaacg cacttattta gtgcatccat ctgctatccc cagctgatta agtaaatttt
    16081 ttgtatccac atcatcacac aatcgttaca taaagattgt tttttcatca ggttttacgc
    16141 taaataatca ctgtgttgag tgcacaattt tagcgcacca gattggtgcc ccagaatggt
    16201 gcatcttcag ggtattgccc tataaatcgt gcatcacgtt tttgccgcat ctcgaaaaat
    16261 caatgagttg caaaattggc acgatttttt catatatgtg aatgtcacgc aggggatcgt
    16321 cccgtggata gaaaaaagga aatgctatga agtctgtatt aaaagtttca ctggctgcac
    16381 tgaccctggc ttttgcggtt tcttctcatg ccgcggataa aaaattagtt gtcgcaacgg
    16441 ataccgcctt cgttccgttt gaatttaaac agggcgataa atatgtgggc tttgacgttg
    16501 atctgtgggc tgccatcgct aaagagctga agctggatta cgaactgaag ccgatggatt
    16561 tcagtgggat cattccggca ctgcaaacca aaaacgtcga tctggcgctg gcgggcatta
    16621 ccatcaccga cgagcgtaaa aaagcgatcg atttctctga cggctactac aaaagcggcc
    16681 tgttagtgat ggtgaaagct aacaataacg atgtgaaaag cgtgaaagat ctcgacggga
    16741 aagtggttgc tgtgaagagc ggtactggct ccgttgatta cgcgaaagca aacatcaaaa
    16801 ctaaagatct gcgtcagttc ccgaacatcg ataacgccta tatggaactg ggcaccaacc
    16861 gcgcagacgc cgttctgcac gatacgccaa acattctgta cttcatcaaa accgccggta
    16921 acggtcagtt caaagcggta ggtgactctc tggaagcgca gcaatacggt attgcgttcc
    16981 cgaaaggtag cgacgagctg cgtgacaaag tcaacggcgc gttgaaaacc ctgcgcgaga
    17041 acggaactta caacgaaatc tacaaaaaat ggttcagtac tgaaccgaaa taataacgct
    17101 acacctgtaa agcgcactgg cagttccctc tcccccctgg ggagaggatt agggtgaggg
    17161 gcgcaaaccc gctccggggc cattaattac cctgaatttg attatttaca ccacggtaac
    17221 aggaacaaca tatgcagttt gactggagtg ccatctggcc tgccattccg cttctgattg
    17281 aaggtgccaa aatgaccctg tggatttcgg tcctcggtct ggctggcggt ctggtaatcg
    17341 gattgctggc aggttttgca cgcaccttcg gtggttggat agccaaccac gtcgcgctgg
    17401 tctttattga agtgatccgc ggcacaccta tcgtcgtcca ggtgatgttt atctatttcg
    17461 ccctgccgat ggcgtttaac gacttacgca tcgacccatt tactgcggcg gtggtcacca
    17521 tcatgatcaa ctccggcgcg tacattgcgg aaatcacgcg tggtgcggtg ctgtctatcc
    17581 acaaaggttt tcgtgaagca ggactggcgc tcggtctttc acgttgggaa accattcgct
    17641 acgtcatttt accgctggca ctgcgtcgta tgctgccgcc gctgggtaac cagtggatca
    17701 tcagcattaa agacacctcg ctgtttattg ttatcggcgt ggcggaactg acccgtcagg
    17761 ggcaagaaat tattgccggt aacttccgcg cccttgagat ctggagcgcc gtggcggtgt
    17821 tctatctgat tattaccctg gtgctgagct ttattctgcg tcgtctggaa agaaggatga
    17881 aaatcctgtg attgaattta aaaacgtctc caagcacttt ggcccaaccc aggtgctgca
    17941 caatatcgat ttgaacattg cccagggcga agtcgtggtg attatcgggc cgtccggttc
    18001 cggtaaatcg accctgctgc gctgcatcaa caaactggaa gaaatcacct ccggcgatct
    18061 gattgtcgat ggcctgaagg tcaacgatcc gaaagttgac gagcgcctga ttcgccagga
    18121 agcaggtatg gtgttccagc agttttacct cttcccgcat ctgacggcgc tgggaaacgt
    18181 catgtttggt ccgctacgcg tgcgtggcgc gaacaaagaa gaggcggaaa aactggcgcg
    18241 tgagctgctg gcgaaagtcg gtctggcaga acgtgcacat cactaccctt ccgaactttc
    18301 tggtggtcaa cagcagcgtg tggcgattgc ccgcgcgctg gcggtgaagc cgaaaatgat
    18361 gctgtttgat gaaccgactt ccgctcttga cccggaactg cgccatgaag tgctgaaggt
    18421 tatgcaggat ctggctgaag aagggatgac gatggtgatc gtgacccacg aaatcggttt
    18481 tgccgagaaa gtagcttcgc ggctgatctt tatcgacaaa ggccggattg cggaagatgg
    18541 caatccgcag gtgttgatca agaacccgcc gagccagcgc ttgcaggaat ttttgcagca
    18601 cgtctcttaa taagacacat tgcctgatgc gctacgctta tcaggcctac aggatatctg
    18661 gcaacttatt aaaattgcat gaacttgtag gacggataag gcgttcacgc cgcatccggc
    18721 aaaaaagccc gcacgttgtc agcaacctgc ttaatatccc ttcctccctt tcacccgaaa
    18781 gggaggcaca ccagattcct ctcatttaaa atcgcccctc ccccagcatc tatacttatc
    18841 tttttgctct gttttctcac tggaggagtc atgcggtgga tcctgttcat cctcttctgc
    18901 ctgctgggcg cacctgccca cgcggtatcc atacccggcg ttacaaccac aacgacaacg
    18961 gactcaacga ctgaaccggc cccggaaccg gatatcgaac aaaaaaagcg gcctatggcg
    19021 cactggcgga tgtgctggat aacgacacct cgcgtaaaga gttgatcgac cagttgcgta
    19081 ctgtcgccgc gacgccccct gcggaaccgg tcccgaagat cgtgccgccg acgctggttg
    19141 aagagcaaac cgtgctgcaa aaggtcaccg aagtcagccg ccattatggt gaagcccttt
    19201 ccgcccgctt cgggcaactt tatcgcaata tcaccggctc cccgcataag ccatttaatc
    19261 cacaaacctt cagcaatgcg ctgagccatt tttcaatgtt agcggtatta gtgtttggtt
    19321 tttactggct gattcgcctg tgcgcactgc cgctatatcg caaaatgggt cagtgggcga
    19381 ggcaaaaaaa tcgtgagcgc agcaactggt tgcagcttcc ggcgatgatt attggggcgt
    19441 ttattatcga cctgctgtta ctggccctga cattatttgt cggccaggta ttaagcgaca
    19501 acctgaatgc aggcagtcgc accatcgctt tccaacaaag tttgtttctc aacgcctttg
    19561 ccctgattga atttttcaaa gccgtactac gcctgatttt ttgcccaaac gtggcggagc
    19621 tgcgcccgtt cacgattcag gacgagagcg cccgttactg gggtcgtcgc ctgagctggt
    19681 taagcagcct gattggctat ggcctgattg tggccgtgcc gattatctct aatcaggtga
    19741 atgtacagat aggtgcgctg gcgaacgtca tcattatgct gtgcatgacc gtctgggcgt
    19801 tgtacctgat cttccgaaat aaaaaagaga ttacccagca tatgctcaac tttgcggagc
    19861 attcgctggc ttttttcagc ctgtttatcc gcgcctttgc gctggtgtgg cactggctgg
    19921 caagcgccta ttttatcatg ctgtttttct tttcgttgtt cgatccgggc aacagtctga
    19981 aatttatgat gggtgcaacg gtgcgcagcc tggcgattat tggtatcgca gcgtttgttt
    20041 ccggtatgtt ttcccgctgg ctggcgaaaa ccatcaccct ctcgccacat actcagcgta
    20101 actatccgga gctacaaaaa cggctgaatg gctggctttc ggcggcgctg aaaacggcgc
    20161 gtattctgac cgtctgcgtg gcagtaatgt tgctgttgag cgcatgggga ttgttcgatt
    20221 tctggaactg gctgcaaaac ggcgcggggc agaaaaccgt agatatcctg atccgcatcg
    20281 cactcattct tttcttctcg gcggttggct ggacggtgct cgccagtttg atcgaaaacc
    20341 ggctggcttc ggatattcat ggccgcccgc tacccagcgc ccgtacgcgt accctgctga
    20401 cgctgttccg taacgcgctg gcggtgatta tcagtaccat caccatcatg attgtgttgt
    20461 cggaaatcgg cgtcaatatc gcgccattgc tggcaggtgc cggggcatta ggtctggcta
    20521 tctcgtttgg ttcgcaaacg ctggtaaaag atattatcac cggggtattt attcagtttg
    20581 aaaacggcat gaacactgga gatttggtga ctatcgggcc gttgaccggc acagtggaac
    20641 ggatgtcgat tcgctccgtg ggcgtgcgcc aggataccgg ggcgtatcac atcattccgt
    20701 ggtcttcgat aaccaccttt gctaacttcg tccgcggtat tggttcggta gtggcaaact
    20761 acgatgttga tcgccatgaa gatgctgata aagccaatca ggcactgaaa gatgcggtaa
    20821 cggaattgat ggaaaacgaa gaaattcgcg ggctgattat tggtgaaccg aactttgccg
    20881 ggattgtcgg cttaagcaat accgcgttta cactgcgtgt ttcgttcacc acgctgccac
    20941 tcaaacagtg gacggtacgc tttgccctcg acagccaggt gaaaaaacat ttcgacctgg
    21001 cgggcgttcg cgcgccagtg cagacttatc aggtgctgcc tgctccgggc gcgaccccgg
    21061 ctgaaccgct gccgccgggg gagccaacgc tttaacgttg gcgattaaca aaacggcggc
    21121 gctgctcgtc gttcataaag gtccaggcaa taaagcgact ctgcttttgc ccctgggcca
    21181 tctctttttt taccaccttc accgcgccca cgtcggtcag agcacgatac aacggcggta
    21241 agttttcacc acgagatacc agcgaggtaa accacatcac ctgcttcgcg aagcctttgc
    21301 tctcttcaat catctttttg ataaagttga cttcaccgcc ttcacaccac aactcctgtt
    21361 gctgaccacc aaagttcagt gcatcgtctt tgttcagccc caggttacgg cgtttacgct
    21421 cactacctgc ccgcgccgca gcggcggaat cgtggaatgg cgggttacac aaggtcgcgt
    21481 cgtattgctc gtttttgtgg atgatgccgt taaaaatcgc cccactctct ttttgccgac
    21541 gcagacgaat ggcgcggtta agccccggat tagcactgat aatcgcctgc gcactgctta
    21601 acgcctggct gctggtttca ctaccggtaa aacgccagcc atattcatgt acgccaatca
    21661 gcggataaat acagttcgca ccaacgccga tatccagaat gctggcattc gctggaattg
    21721 ttccgcttgc ttcagccagt aaatcggcaa ggtgatgaat ataatccgcc cggcccggta
    21781 ctggtgggca gagaaaaccg tcggggatat cccagttcgc tacggcgtaa aaatgggcca
    21841 gcaacgcctt attgagcgcc ttcaccgcca gcggattggc aaagtctacg ctttgctccc
    21901 ctgcgggtgt aagcgtgagg aattgcctga gttcaggatt gacctgacaa agcgtggcga
    21961 gatcgtagcg gctgtgatga cggttgcgcg gatgcaaccc cggtttctgg gcggacatag
    22021 cattctcctt taagcagcgg cgtaagatac ccgcataacg cgacagggta aatatctggc
    22081 tgcgtttatc attccccaaa accttaacat ggctatggtt tatggcaatt catcataaaa
    22141 atcagcatta tgcatgtcat tgatttgtct tattttgtaa aaatatatcg ttcaaaaaat
    22201 aagcatttct ctgtcaattt tttgcgtcat ttgcatcgcg caggcgacct acacttatca
    22261 ggcactaccc gttgtattta tgaggatgtc attatgaaaa agtgcctcac actactgatt
    22321 gccaccgtcc tgagcggaat ctctctcacg gcttatgccg cgcaaccgat gagtaacctg
    22381 gacagcggtc agctacggcc cgccggtacc gtttcggcaa ctggcgcatc aaacctaagc
    22441 gatctggagg ataaactggc agaaaaagcg cgcgaacaag gcgcgaaagg ttatgtcatt
    22501 aattccgcag gcggaaatga ccagatgtta ggtactgcaa ccatctacaa ataaccccac
    22561 caacgtaact gcattcgcct ctggatgcag ttttcgcctg ttttgtaatc aaaaaacatc
    22621 aaatatgaac gcaatgtaaa taaatgtatt tctttttcgc gcaatgggtg atagaaaatc
    22681 gcatcaagtg ataatgctta tcaaaattat tatcacttgc acgaacacta tcacgggatt
    22741 aacagtggca tcgcatccgc agagatgctt tctcgtggca gtgaaaattt caacatataa
    22801 gaaaaagtca cctgcaaaat ggaaaacaat cgcaatttcc ctgccagaca atttcattcg
    22861 ctcacgttct ttgccggtct ttgtattggt attacgcctg tggcacaggc actcgccgcc
    22921 gaagggcaag ctaacgcgga tgacacgctg gttgtcgaag catcaacgcc ttcgctttat
    22981 gcgccacaac aatctgccga tccgaaattc tcgcgtccgg tagcggatac tacccgcacg
    23041 atgacggtga tttctgaaca agtgattaaa gatcagggcg caaccaacct taccgacgcg
    23101 ctcaaaaacg tccccggcg
//
LOCUS       DAJTSI010000057        22768 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000057 DAJTSI010000000
VERSION     DAJTSI010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22768
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.057"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1442
                     /locus_tag="OO327_001987"
     CDS             <1..1442
                     /locus_tag="OO327_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6018534.1"
                     /translation="DWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRRNNC
                     YQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWEISQ
                     YNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFATGHL
                     GIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQPYL
                     VINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNCNGN
                     YMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPITVSI
                     DEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLEALL
                     ACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGAGPE
                     ITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            1808..2875
                     /locus_tag="OO327_001988"
     CDS             1808..2875
                     /locus_tag="OO327_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6018535.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2872..5607
                     /gene="rbbA"
                     /locus_tag="OO327_001989"
     CDS             2872..5607
                     /gene="rbbA"
                     /locus_tag="OO327_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR6018536.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREARINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            5607..6731
                     /locus_tag="OO327_001990"
     CDS             5607..6731
                     /locus_tag="OO327_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6018537.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            6790..7065
                     /locus_tag="OO327_001991"
     CDS             6790..7065
                     /locus_tag="OO327_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCR6018538.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            7062..7421
                     /locus_tag="OO327_001992"
     CDS             7062..7421
                     /locus_tag="OO327_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR6018539.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(7541..7942)
                     /gene="nikR"
                     /locus_tag="OO327_001993"
     CDS             complement(7541..7942)
                     /gene="nikR"
                     /locus_tag="OO327_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR6018540.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(7948..8754)
                     /gene="nikE"
                     /locus_tag="OO327_001994"
     CDS             complement(7948..8754)
                     /gene="nikE"
                     /locus_tag="OO327_001994"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR6018541.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            complement(8751..9515)
                     /gene="nikD"
                     /locus_tag="OO327_001995"
     CDS             complement(8751..9515)
                     /gene="nikD"
                     /locus_tag="OO327_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR6018542.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(9515..10348)
                     /gene="nikC"
                     /locus_tag="OO327_001996"
     CDS             complement(9515..10348)
                     /gene="nikC"
                     /locus_tag="OO327_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR6018543.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGH
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(10345..11289)
                     /gene="nikB"
                     /locus_tag="OO327_001997"
     CDS             complement(10345..11289)
                     /gene="nikB"
                     /locus_tag="OO327_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR6018544.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(11289..12863)
                     /gene="nikA"
                     /locus_tag="OO327_001998"
     CDS             complement(11289..12863)
                     /gene="nikA"
                     /locus_tag="OO327_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR6018545.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(12974..13561)
                     /gene="acpT"
                     /locus_tag="OO327_001999"
     CDS             complement(12974..13561)
                     /gene="acpT"
                     /locus_tag="OO327_001999"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR6018546.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(13616..14665)
                     /locus_tag="OO327_002000"
     CDS             complement(13616..14665)
                     /locus_tag="OO327_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6018547.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            14799..16016
                     /locus_tag="OO327_002001"
     CDS             14799..16016
                     /locus_tag="OO327_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6018548.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(16020..16577)
                     /gene="dcrB"
                     /locus_tag="OO327_002002"
     CDS             complement(16020..16577)
                     /gene="dcrB"
                     /locus_tag="OO327_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR6018549.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(16650..17315)
                     /locus_tag="OO327_002003"
     CDS             complement(16650..17315)
                     /locus_tag="OO327_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR6018550.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            17536..17781
                     /gene="tusA"
                     /locus_tag="OO327_002004"
     CDS             17536..17781
                     /gene="tusA"
                     /locus_tag="OO327_002004"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR6018551.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(17883..20081)
                     /gene="zntA"
                     /locus_tag="OO327_002005"
     CDS             complement(17883..20081)
                     /gene="zntA"
                     /locus_tag="OO327_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR6018552.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPAKGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(20155..20781)
                     /locus_tag="OO327_002006"
     CDS             complement(20155..20781)
                     /locus_tag="OO327_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR6018553.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            20922..21281
                     /locus_tag="OO327_002007"
     CDS             20922..21281
                     /locus_tag="OO327_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR6018554.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(21284..21553)
                     /locus_tag="OO327_002008"
     CDS             complement(21284..21553)
                     /locus_tag="OO327_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR6018555.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(21543..22139)
                     /gene="rsmD"
                     /locus_tag="OO327_002009"
     CDS             complement(21543..22139)
                     /gene="rsmD"
                     /locus_tag="OO327_002009"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR6018556.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            22289..>22768
                     /locus_tag="OO327_002010"
     CDS             22289..>22768
                     /locus_tag="OO327_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="cell division protein; signal recognition protein
                     receptor; functions in the targeting and insertion of
                     membrane proteins; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR6018557.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSSEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     "
BASE COUNT         5419 a         5922 c         6222 g         5205 t
ORIGIN      
        1 atgattggca aatagaacgt ttccaacaat ctattcaaga tgacaaaatt tcatttacca
       61 tacaaacgaa tcattcagaa aaatatagta tgctatcggg catgcgcgct catattctac
      121 gtaggaataa ctgttatcag tttattgtga caattaatag taaaaattac ggttgccctc
      181 ttgataatac cgatattaat tggtgcagca ttgtttattt acttaataat atgacagtaa
      241 atgataatgc taatgatgtt gcagttactg agtcgtacaa acccatctgg aactgggaaa
      301 tatcgcaata caatgtattt gatattaaat tcgaaacaat tataaaaccc cagttcgctg
      361 acagaaccta tttctccaat tgttcacctg ttgatcccac aagtaccagg ccgacctatt
      421 ttggcgatac cgatggctca gtcggtgcag ttttgtacgc gctttttgca acaggacatt
      481 tgggaatcat ggctgaagga gaaaatttcc ttagccagtt gcttaatata gaagatgaag
      541 ttctcaacgt attgctaagg gagaacttta atgagcaact tgacactaat gttaatacaa
      601 taatcagtat actgaacaga agagataatg ttttagaaag tcttcagcct tatttggtta
      661 ttaataaaga cgcagtcacg ccatgtacgt ttcttggcga ccaaaccggc gatcgattta
      721 gtaatatctg tggcgatcaa tttatcatcg atttgttaaa acgcataatg agtatcaatg
      781 aaaatgttca tgtattagct ggaaatcatg aaacgaattg caatggtaac tatatgcaaa
      841 acttcacgcg catgaaacca cttgatgagg atacctacga cgggataaaa gattatcctg
      901 tatgtttcta tgaccccaaa tacaaaataa tggcaaatca tcatgggata acttttgatg
      961 atcagcgaaa acgttacatt ataggaccga taactgtctc tattgatgaa atgaccaatg
     1021 ctctcgatcc ggtcgaatta gcggaaatta ttaataaaaa gcatcatgct ataattaatg
     1081 gcaaaaagtt taaaactagc cgggctatct cttgtcgatc ctttaatcgt tatttttctg
     1141 tttcgacaga ttacagacct aaacttgaag cactattagc atgttcacaa atgttaggta
     1201 taaatcaagt tgttgcacat aacggaaatg gtgggagaga acgcataggt gaaacgggga
     1261 cagttttagg tcttaacgcc cgtgacagta aacatgctgg aagaatgttc agcatgcata
     1321 attgccaaat taaccctggt gcagggccag agataaccac tccctggaag tcttaccagc
     1381 atgaaaaaaa cagaaatgga cttatgcctt tgattagaag gcgcacaatg ttacagctct
     1441 gaaccataga caacatgaat attaatgtta ctatctggtt gagactgttg ataaaacgta
     1501 aaaaggaatg ctctgttcct gcaactgaac aggcgtgaaa tgcgatccaa caggatggat
     1561 accgttttgt cagccagttt tatagatcac acttatgagt atgcgtgttt catttacggc
     1621 caggaatgct gatgatgaca acattactac ccgttttcac taagccttcc ccgttggcgc
     1681 tcaatgctct gcgcgctggt cgtatttgtc gtttccttct tatcccggat ggaagaatcc
     1741 gctgaacgtc gttcggcggc agttgtaaca cgcgtcgttt gcttacaggg attatggcgg
     1801 gtcgattatg gataagagta agcgccatct ggcgtggtgg gttgtcgggg cactggcggt
     1861 ggctgccgtc gtggcgtggt ggctgttgcg cccggcgggt gtgccggaag gctttgctgt
     1921 cagcaatggg cgcattgaag cgacggaagt ggatatcgcc agcaaaattg ccgggcgtat
     1981 cgacaccatt ctggtgaaag aaggccagtt tgttcgcgaa ggcgaagtgc tggcgaagat
     2041 ggatactcgc gtgttgcagg aacagcgact ggaagccatc gcgcaaatca aagaggcaca
     2101 aagcgccgtt gccgccgcgc aggctttgct ggagcaacga caaagtgaaa ctcgtgccgc
     2161 acagtcgctg gtcaatcaac gccaggccga actggactct gttgccaaac gtcatacgcg
     2221 ttcccgctcg ctcgcccaac gaggggctat ttctgcgcaa cagctggatg acgaccgcgc
     2281 cgccgctgag agcgcccgag ctgcgctgga atcagcgaaa gcccaggttt cggcttctaa
     2341 agcggctata gaagcagcac gcaccaatat cattcaggcg caaactcggg tcgaagcagc
     2401 gcaagccact gaacggcgca ttgccgcaga tatcgatgac agtgaactga aagccccgcg
     2461 tgacggacgc gtgcagtatc gggttgccga gccaggggaa gtgctggcgg caggcggtcg
     2521 ggtgctgaat atggtcgatc tcagcgacgt ctatatgacc ttcttcctgc caaccgaaca
     2581 ggcgggcacg ctgaaactgg gcggtgaagc ccggctgatc ctcgatgccg cgccagatct
     2641 gcgtattcct gcaaccatca gttttgtcgc cagtgtcgcc cagttcacgc caaaaaccgt
     2701 cgaaaccagc gatgaacggc tgaaactgat gttccgcgtc aaagcgcgta tcccaccgga
     2761 attactccag cagcatctgg aatatgtcaa aaccggttta ccgggcgtag cgtgggtgcg
     2821 ggtgaatgaa gaacttccgt ggcctgacga cctcgtggtg aggttgccgc aatgacgcat
     2881 ctggaactgg ttcccgtccc gcctgtcgcg caactggcgg gcgtgagcca gcattatgga
     2941 aaaaccgttg cgctgaacaa tatcactctc gatattccgg cccgttgcat ggtcgggctg
     3001 attggtccgg atggcgtcgg gaagtcgagt ttattgtcac tgatttccgg tgcccgcgtc
     3061 attgagcagg gcaatgtggt ggtgctgggt ggcgatatgc acgacccgaa gcatcgccga
     3121 gacgtctgcc cgcgcatcgc ctggatgccg caggggctgg gcaaaaacct ctaccacacc
     3181 ttgtcggtgt atgaaaatgt cgattttttc gctcgcctgt tcggtcacga taaagcggag
     3241 cgggaagcgc gaattaatga gctgctgacc agcaccgggt tagcaccgtt tcgcgatcgt
     3301 ccggcaggta aactctccgg cgggatgaag caaaaacttg ggctgtgctg cgcgttaatc
     3361 cacgacccgg aactgttgat ccttgatgag ccaacaacgg gggttgaccc gctctcccgc
     3421 gcccagttct gggatctgat cgacagtatt cgccagcggc agagcaatat gagcgtgctg
     3481 gtcgccaccg cctatatgga agaggccgaa cgcttcgact ggctggtagc gatgaatgcc
     3541 ggagaagtgc tggcaaccgg cagcgccgaa gagctgcggc agcaaacgca aagcgccacg
     3601 ctggaagaag catttattaa tctgttaccg caagcgcaac gccaggcgca tcaggcggta
     3661 gtgatcccac cgtatcaacc tgaaaacgca gagattgcca tcgaagcgcg cgatctgacc
     3721 atgcgttttg gttccttcgt tgccgttgat cacgtcaatt tccgcattcc acgcggggag
     3781 atttttggat tccttggttc gaacggctgc ggtaaatcca ccaccatgaa aatgcttacc
     3841 ggactgctgc ccgccagcga aggtgaggcg tggctatttg ggcaaccggt tgatccaaaa
     3901 gatatcgata cccgccgtcg tgtgggctat atgtcgcagg cgttttcgct ctataacgaa
     3961 ctcaccgtgc ggcaaaacct ggagttacat gcccgtttgt ttcacatccc ggaagcggaa
     4021 attcccgcgc gagtggctga aatgagcgag cgttttaagc tcaacgacgt tgaagatgtt
     4081 ctgccggagt cattgccgct cggcattcgc cagcggcttt cgctggcggt ggcggtgatt
     4141 catcgcccgg agatgttaat cctcgatgag cctacttctg gtgtcgatcc ggtggcgagg
     4201 gatatgttct ggcagttgat ggtcgatctc tcgcgccagg acaaagtgac tatcttcatc
     4261 tccacccact ttatgaacga agcggaacgt tgcgaccgca tctcactgat gcacgccgga
     4321 aaagtgcttg ccagcggtac accgcaggaa ctggttgaga aacgcggagc cgccagtctg
     4381 gaagaggcat ttatcgccta tttgcaggaa gcggcagggc agagcaacga agccgaagcg
     4441 ccgcccgtgg tacacgacac cacccacgcg ccgcgtcagg gatttagcct gcgccgtctg
     4501 tttagctaca gccgccgcga agcgctggaa ctgcgacgcg atccagtacg ttcgacgctg
     4561 gcgctgatgg gaacggtgat cctgatgctg ataatgggtt acggcatcag tatggatgtg
     4621 gaaaacctgc gctttgcggt gctcgaccgc gaccagaccg tcagtagcca ggcgtggaca
     4681 ctcaacctct ccggttcccg ttactttatc gaacagccgc cgctcaccag ttatgacgag
     4741 cttgatcgtc ggatgcgtgc gggcgatatc acggtggcca ttgagatccc gcccaatttc
     4801 gggcgcgata tcgcgcgtgg tacgcctgtg gaactcggcg tctggatcga cggagcgatg
     4861 cccagtcgtg ccgaaacggt aaaaggttac gtgcaggcaa tgcaccagag ttggcttcag
     4921 gatgtggcga gccgacaatc gacacccgcc agccaaagcg ggctgatgaa cattgagacg
     4981 cgttatcgct ataacccgga cgtaaaaagc ctgccagcga ttgttccggc ggtgatcccg
     5041 cttctgctga tgatgatccc gtcaatgcta agcgccctta gcgtggtgcg ggaaaaagag
     5101 ctagggtcga ttatcaacct ttacgtgacc cccaccaccc gtagcgaatt tttgcttggc
     5161 aaacagctgc cgtacatcgc gctggggatg ctgaactttt tcctgctctg cggcctgtcg
     5221 gtgtttgtgt ttggcgtacc gcataaaggc agtttcctga cgctcacctt ggcggcgctg
     5281 ctgtatatca tcattgccac tggaatgggg ctgctgatct ccacctttat gaaaagccag
     5341 attgccgcca ttttcggaac ggcgattatc acattgatcc cggcgacaca gttttccggg
     5401 atgatcgatc cggtagcttc gctggaaggg cctggacgtt ggatcggcga ggtttacccg
     5461 accagtcatt ttctgactat cgcccgcgga acgttctcga aagcgctgga tctgactgat
     5521 ttgtggcaac tttttatccc gttgctgata gccatcccgc tggtgatggg cttaagcatc
     5581 ctgctgctga aaaaacagga gggatgatgc gccatttacg caatattttt aatctgggta
     5641 tcaaagagtt gcgcagtctg ctcggtgata aagcgatgct gacgctgatt gtcttctcgt
     5701 ttacggtgtc ggtgtattcg tcagcgaccg ttacgccagg atcgttgaac ctcgcgccga
     5761 tcgccattgc cgatatggat caatcgcagt tatcgaaccg gatcgttaac agcttctatc
     5821 gtccgtggtt tttgccaccg gagatgatca ccgccgatga gatggatgcc ggactggacg
     5881 ccggacgcta taccttcgcg ataaatattc cgcctaattt tcagcgtgat gtcctcgccg
     5941 gacgccagcc ggatattcag gtgaacgtcg atgccacgcg catgagccag gcatttaccg
     6001 gcaatgggta tatccagaat attatcaacg gtgaagtgaa cagctttgtc gcgcgctacc
     6061 gtgataacag cgaaccgttg gtatcgctgg aaacccggat gcgctttaac ccgaaccttg
     6121 atcccgcgtg gtttggcggg gtgatggcga tcatcaacaa cattaccatg ctggcgattg
     6181 tattgaccgg atcggcgctg atccgcgagc gtgaacacgg cacggtggaa catttactgg
     6241 tgatgccgat aacgccgttt gagatcatga tggcgaagat ctggtcgatg gggctggtgg
     6301 tgctggtggt atcgggatta tcgctggtgc tgatggtgaa aggtgtactg ggcgtaccga
     6361 ttgaaggctc gatcccgctg tttatgctgg gcgtggcgct cagtctgttt gccaccacgt
     6421 caatcggcat ttttatgggg acgatagcgc gttcaatgcc gcaactgggg ctgctggtga
     6481 ttctggtgct gctgccgctg caaatgcttt ccggtggatc cacgccgcgc gaaagtatgc
     6541 cgcagatggt gcaggacatt atgctgacca tgccgacgac acactttgtt agcctcgcgc
     6601 aggccatcct ctaccggggt gccggattcg aaatcgtctg gccgcagttc ctgacgctga
     6661 tggcaattgg cggcgcgttt ttcaccatcg cactgctgcg attcaggaag acgattggga
     6721 caatggcgta aggaagggaa acttgacttc atactgtgat gtcataaagt gaagtcataa
     6781 ggacatgcca tgagaaccaa ggtacaggct ttacggaaga aacaaaaaaa tacattggat
     6841 caaatattta aaactcctgt tcctcaaggg atcaagtggt ctgatataga gtcactggtt
     6901 aaagcattag gcggagaaat taaggaagga agaggttcgc gttgcaagtt catactaaat
     6961 atgagcgttg cgtgtttcca tcggcctcat ccgtcgccag ataccgataa aggcgctgta
     7021 gaaagcgtgt gtgactggtt actaagtata ggggtaaaac catgatcaaa ctgaagacgc
     7081 caaactctat ggagattgcc gggcaaccag ctgttattac ctacgtgcca gaactgaatg
     7141 catttcgtgg caaattcctg ggcttgtcag gttattgtga ttttgtctct gacagtattc
     7201 aggggctgca aaaagaagga gagctttccc tgcgagagta tcttgaggat tgtaaagctg
     7261 cgggtattga gccgtatgcg cgtacagaaa agattaagac ctttacgtta cgctatccag
     7321 aatcgctgag tgaacgcctt aataatgcgg ctgcgcagca acaagtctct gttaatacct
     7381 atattattga aacactgaat gagcgtttga atcatttata aattttagcg acgtacatta
     7441 cgcagaatat ctgcctttac aggatctatc tatgcaactg aacttaattt tataaattaa
     7501 gttcagttaa tatcttttct ttccttgttt tagaaataac tcaatcttcc ttcggcaggc
     7561 actgcaaatg cccgtgccgc acgccgcgct gggcgataac gtcatcggca aaatgctgca
     7621 cgtcacccat gtcacctttc aacacggcga tttccagaca gtcgtcgtgg ttgatgtgca
     7681 catgtagcgt ggcgacggag aggtcgtggt gatgatgctg ggtggaaaca atacggctgg
     7741 ctaagtcgcg tttttcgtgt tcatacacat acgacagcac cgcgaaacct tgtgtgccgt
     7801 gctgctgggt agcttcctgc gccagggcac tacgcagaat atcgcggata gcttcggaac
     7861 ggttgttata accacgacgc tggctcaggc tgtccagcgt ctccagtaaa tcgtcatcaa
     7921 gcgtgatggt gactcgttgc attggcgtta aaccttttct gaggtgcgac ggcgcacggg
     7981 gaatgcgggt aataccgcgt tttgtagcac acgtccggcg tcagaggaaa aggttaattt
     8041 atctcccacc gcctgggttt cggcgatttg tccgttgtcc atcaccatca ctcgctggca
     8101 aaagcgttcc accaggcgta agtcatgagt gatgaacagg caggcagtgc caaactgttg
     8161 ttgtagcttt ttcagcaggc gaatgacgcc cgcctgtaac acgagatcaa ggttagaaac
     8221 ggcttcatcc agaatcagca atttcggttc gaccgccagc gcgcgtgcca ggcagacgag
     8281 ctggagctgg ccgccgctta actgcggtgg gcgtttgtcg agaacgctgt catcgagatc
     8341 gaccgccttc agcatttcgc tggcgcgcgc cagttgttcg gattttttca gtgatagcag
     8401 gtggcgcatc ggttcacgca ggatctcgcg cacggttttg cgcggattca cggcactgat
     8461 ggagtcctga aacaccatct gaatatcacg gcggaaggct ttacgttggg cgcgattaag
     8521 tttcgccagc gattcgccgt gccagctaat attcccttgt gagggagact ctaagcctac
     8581 cagcagccgc gcgagggtgc tttttccgca gccgctgcgt cccagcaggg cgacggtttc
     8641 gccgcttttc agggtcaggg aaacgttatt cagcaccgcc tgatgttgat gttttccgct
     8701 aaatccaccg tgggcatagt gatgggaaag gccgcagacg ttaagtaagg tcatgatgcc
     8761 agctccatac cgtagagggc gagatgagcg gaaaccaggc tgcgcgtcac cgtatgtttg
     8821 ggggcgttaa acagcgtttc tacatcgccc tgttcgacaa ttttaccttg tgacatcacc
     8881 gccacgtcat ccgccagacg cgccacaacg cccatatcat gggtgaccag cagcattccc
     8941 ggcgcttgtt tttgcataat gctttccagc agatcgagga tgcgcgcctg tgctaccacg
     9001 tcgaggtcgg tggtcggttc atcggcgatg ataaacggtg attcacacag caccgccatc
     9061 gcaatcatca tgcgctgcaa catgccgccg ctcatctcga acgggtacag cttcagcacg
     9121 cgcgcggcgt tttccagccc caccgcttct atggcagcgg taagcgtagc gtcatcggcg
     9181 ggtttcccta acgccaggca ggtttcccgc gcgtgggtgt gcatggtgtg cagtggatta
     9241 aaggcgctgc gcgggttctg catgatggtg gcaattttga tgccgcgcag ggcgtaaggc
     9301 gaaaccggtt tgccatcggc taaaatttcc cccgccgtct ggcgaacgcc agcgggcaaa
     9361 atgcccagcg tcgcggcgca ggttaatgat ttcccgctgc cgctaccgcc gactaacgcc
     9421 agcacgcgcc cgcgttgcag ggttaacgat acaccgtgta ccagcggctg cgcggcctgt
     9481 agcgtgatat tacgtagttc aatctgttgc ggcattagtg tgcgtgctcc gtcaccagat
     9541 gaggatccag atgatcgcgc agtgcgtcac ccaccaggtt aaaggccatc acgctgataa
     9601 acagcgccag ccccggccag aacatttgca gcggctgggt ccagatatac tggcgcgcgt
     9661 cgttaatcat cacgccccat tcggcggtcg gcgcggtcac accgaggcca aggaaagaca
     9721 tccccgcgac gtgcagcatc atatggccga tatccagcgt tgccagcacc agcagcgaag
     9781 ggatcaccgc gcctgccaga tgatcgacaa acacccgcac atggcccgca ccggaaagcc
     9841 gtgacgccag cacaaactcg cgttggcgta gtgaaatcac caagctgcgc accatgcgtg
     9901 cataccacgc ccagtgcgac agggcgatgg cgataattac gttggtcagc ccggtgccga
     9961 gcacgccaac cataaagaac gacagaatcg aggtcgggaa ggtcataaac atatcggcga
    10021 cgcgcatggt ggcctgatca acgtgcccgc caatcaaccc ggcgctgccg ccaataacca
    10081 gccctaatgt cagcaccagc agcaggcagg ccattaccga accgagcgac acgcgggtcg
    10141 ctgccatcag ccgcgagaaa atatcgcgac ctaagtgatc ggtgcccagc cagtgctgcg
    10201 catccggcga aagcaggcac gacggcaaat caattgcctg tggatcgtac ggctgccacc
    10261 actggctggt gagcgcaatc agcgccagca gggcgataat gatcagcgcc aggcgtaccg
    10321 accagcggga agagaggaaa aagttcacgc gtgcgctcct tcatgacgac gaatgcgcgg
    10381 gtccagcgcg gcgttgagca aatcgacaat caaattacag accacaaaaa ccaccaccat
    10441 catcagcgta aagcactgaa tcaccggata gtcacggtta aaaatcgccg acaccgcata
    10501 gcgcccgacg ccaggccagg caaagatgtt ttcgataatc atcgtcccgc caatcaattc
    10561 gccaatgtgc atccccacgg cggtgatcat cggcagcgag gcattgcgca gaatatggcg
    10621 gcgttcggtc tgtttgtcat tcaggccgcg tagccgcgcc caggtgacgt gacgctgacc
    10681 ggcgacgtcc agcatactgg cgcgcagcag tcgcgcgtta atcgccagcg acataaaggc
    10741 gatggaaacc gcaggtaaaa tgatgtgctg ccagctgccg tagcccatcg cgggtagcca
    10801 ttgcagatac accgaaaacg ccatcactag caaaaatgcc agccagaagt tgggcatcga
    10861 cacgccaaga aacgcgataa agcgcacggc gaaatccggc aaacgatcgc ggtggcgcgc
    10921 cgcccagata ccgagcggta cggaagtgag cagaattaat accagcgccg cacctgcaag
    10981 ttccagcgtg gcgggcagga agttcagcat atcgtccagt accgggcgtt ggctggcgaa
    11041 tgagatacca aagtcaagat gcagcgcctt ccacaaccag gtaccgtact ggacgtacag
    11101 cggctgatcc agcccgagca tggtgcgggt agaggccagc atctccggcg tcggcggcag
    11161 gttagataaa cgcagataat cgagcgccgg gtcgccggta ccgaggcgca gcatcagaaa
    11221 aatgatcacc gaggcggcga gcaccatcgg gatcagcagc agaaagcggc gtaatacgta
    11281 acgcaacatt aaggtttcac cggtttaatc tgttcgaacg gaatttcggt ggcgatcggc
    11341 gcgtagggga tattacccag ctccggtttt gataccacca tcatcgagat gtagctgata
    11401 ggcagataaa ccgcctcgtc atgcagacgg gtcagaatgt cgcgatacag cgcctgacgt
    11461 tgcgtttcgt catgggtcgc cagcacttcg ccgatctctt tatcaatcag cggcttgtcg
    11521 gctaatcctt gctgtgcctg gaagtcagcg tgcgacggta cgcgcattga actcaggaag
    11581 gcgtgtggat cgtatggcgc gccccaggtg cggtggaaaa tcatgccaaa acgaccgtcg
    11641 cgctgacgag catagatact gctctcttct tcgccaatca gcgagacatc tgcgccaatc
    11701 tggcgcatat cagcctgaat gatttccgcc atcgatttgc ttaacgcatc ggtgccgatg
    11761 aacgacagtt caatgcgcag cggctgacca tttttctcgc ggatgtcttt gcccgcaggc
    11821 agcgtccaac cggctttttc cagcaactct ttcgctttct gcggatcgta ctggcgcggt
    11881 ttcaggccga gattagcgta aggcacagaa ggggcaaaca gggtgtcggc gacctgctgg
    11941 gtgccatata acgcgttatc aatcagcgat tttttgttta ccgcgtaatt aagagcttca
    12001 cgtactgcca gctcgttggt gggggctttg gcggtattga gcgccagcat cacggtttcg
    12061 atcggctgtg acagttgggt gtggtaagcc ggattctggc taaagcgggc gaaggtatcg
    12121 agcggtaata acccttcgtt tccgtacagc aggtcgatat cgccagtttc aaacgccacc
    12181 gcgcgggtag tcgggtccgg gatgacgtta aaggtgatct ttttaatcgc tggcttttcg
    12241 ccccagtagt tttcgttacg gacgaagaca tcgtactgat ttagtttcga ttcctgcaaa
    12301 atccacggtc cggtgccaat cggcgcttta attccgttca tggtttcatg gtttttaaac
    12361 tgcgagggag cgataaaacg gaaaggacgg ggcagggcca gttcttgcag gaaaggatag
    12421 taggcgcttt tcagggtaat ttgcagctct gttttattga gtgctttaac atcaacaatc
    12481 tggtttgcca gctccagcca ggcgtgacgt tgacggttat cgagcactgc gcggaagttt
    12541 tctgccgccg cctcggcatc gaacggttca ccgttggaga atttcacgtc atcacgcagg
    12601 gtgaaggtcc aggttttacc atcttctgaa tgagtccagc tttttgccag ccacgggatc
    12661 accgaaccgt ctgcctgata tttcaccaat ggttcataaa ccatgctctg ggcgaacatc
    12721 tggttaggcg tgtaaaggtg cgggtttagt ggcccgacat tcaccggcca ggcggtggtg
    12781 atttcatctg gtgcagcggc atggacgata aaagacgcac aagccagcag cgcaaataga
    12841 gtgcggcgga gtgtggagag catggtaacc ccaatggatt aaaatagatg gcggaaataa
    12901 gtatgacgat tttaagtatt cgtcatactg attacctgtt ctggatcaat agatgggcag
    12961 tcgggtgggc ggatcagtta actgaatcga tccattgcac actgtcggcg gtgagcgtaa
    13021 agggggtggg ggtgcagatc gcaaggctta aattttctag ctgacaatgg ctgactgaca
    13081 gcgaggagtg ataggtgcta tctacgctga cgatttgcca ggcgctgccg ccacgctgtt
    13141 taacgatggc ttctttgcgc gtccagatgc gccagaacat ttccagttgc tgatcaggat
    13201 gcacggcgtc catttcagcg tgttccccga ggctgaacac ggcgttcgcc agccagcgcc
    13261 agttgacgcg cgggcgaatc acttcgatat cgcagccgac ttcgccttca tcgctcaaca
    13321 gcagagcgat atcgtcaccg ctatggctta agttgaacca tagcggcgtt tccggcgcga
    13381 atgcaggttt gccttgttcg ccatagatga tctccggtag cggggaaagc gtgtgcgaaa
    13441 gcaatgtacg ccccgccagc cagcgttcgc gtcgtggacc ttgcggtgct tgctcgcgta
    13501 aacccggtgg cagtggagct gcgcttaagg tcgaaacttt ccccagaact atccgataca
    13561 tatcagggcc aacgtttaat ggaaaatgaa agtgcgtatc gtatcacttg tcgcctcatc
    13621 ccggtaaccg acttttcggt ctgcccggcc ccagtaaaat cgccagtttg ctgccacctt
    13681 tcgtcgtttc catccagatt ttacacacgc tggttaatgg caccgaaagt agcatcccta
    13741 ccgggccgag cagccatccc cagattaaca atgaaagaaa taccaccatg gtggacatcc
    13801 ccaggcgatg gcccatcatc cgtggttcta aaatattgcc gatgaccata tggaccacta
    13861 aaaacaatgc gccgaccaga atacattcgt aaacaccatt aaacagcagc acctgaatca
    13921 ttggcggtac ggcggaaatt accgcgccga tattgggcac gtagttgagc aaaaacgcca
    13981 gtactgccca catcagcgca aactgtacgc ccattagcgc cagccccagc cagacgatga
    14041 cacctgtcca taaactgagt agcgtcttta acgccagata atgcgaaacg cctttaagtg
    14101 cgcggtgtaa tcctgcgatg tgaatctgtg ggttattcag cgcaaaacgc attttgtaag
    14161 ggacgtggcg cacttcaaac agcataaaaa ctacggtcat caccagcaaa agcacgctcg
    14221 ccattgcccc ggaaagcccg gtcattagcg ctgtggtgaa ggtaaccact ttttccgagt
    14281 ccatccgctg cagcattcgc tccggcgaca tatgcaaatt aagaaaaggc aacatctcct
    14341 gcaatttaaa aagtttgcgc gtcagctcct tattaaactt cggcagcata gagataaatt
    14401 cgttaaacga tgccgccagt acgccgacca gcgcggttag tgcgatcagc atcaccacca
    14461 ctacaatcgt aatggcaacg gggcgttgta ctccccgacg aataaaccag gtaacgagcg
    14521 ggttgaggac gatggcaaaa aacagcgcca gtaacagctg cacaatgata tctgccgctg
    14581 cgtgaatgcc cgcgaggatc actaccagcg aggccaactt gagcagaatg tgcatgcccg
    14641 ttttatcggg ttgaggggtt tccattgggg cttccttgtg actttttgtg ttaagtgtag
    14701 cgcgggagtc acgccagcaa tattctgatt cttactgctg attttcacgt cagcacatgg
    14761 taaaaatgaa acactgttgt aaaaatgtgg tgatcctcat gcccgaaccc gtagccgaac
    14821 ccgcgctaaa cggattgcgc ctgaatttgc gcattgtctc cattgtcatg tttaacttcg
    14881 ccagctacct caccatcggg ttgccgctcg ctgtattacc gggctatgtc catgatgtga
    14941 tgggctttag cgccttctgg gcaggattgg ttatcagcct gcaatatttc gccaccttgc
    15001 tgagccgccc tcatgccgga cgttacgccg atttgctggg acccaaaaag attgtcgtct
    15061 tcggtttatg cggctgcttt ttgagcggtc tggggtatct gacggcagga ttaaccgcca
    15121 gtctgcctgt catcagcctg ttattacttt gcctggggcg cgtcatcctt gggattgggc
    15181 aaagttttgc cggaacggga tcgaccctat ggggcgttgg agtggttggc tcgctgcata
    15241 tcgggcgggt gatttcgtgg aacggcattg tcacttacgg ggcgatggcg atgggtgcgc
    15301 cgttaggcgt cgtgttttat cactggggcg gcttgcaggc gttagcgtta atcattatgg
    15361 gcgtggcgct ggtggccatt ttgttggcga tcccgcgtcc gacggtaaaa gccagtaaag
    15421 gcaaaccgct gccgtttcgc gcggtgcttg ggcgcgtctg gctgtacggt atggcgctgg
    15481 cactggcttc cgccggattt ggcgtcatcg ccacctttat cacgctgttt tatgacgcta
    15541 aaggttggga cggtgcggct ttcgcgctga cgctgtttag ctgtgcgttt gtcggtacgc
    15601 gtttgttatt ccctaacggc attaaccgta tcggtggctt aaacgtagcg atgatttgct
    15661 ttagcgttga gataatcggc ctgctactgg ttggcgtggc gactatgccg tggatggcga
    15721 aaatcggcgt cttactggcg ggggccgggt tttcgctggt gttcccggca ttgggtgtag
    15781 tggcggtaaa agcggttccg cagcaaaatc agggggcggc gctggcaact tacaccgtat
    15841 ttatggattt atcgcttggc gtgactggac cactggctgg gctggtgatg agctgggcgg
    15901 gcgtaccggt gatttatctg gcggcggcgg gactggtcgc aatcgcgtta ttactgacgt
    15961 ggcgattaaa aaaacggcct ccggaacacg tccctgaggc cgcctcatca tcttaaaact
    16021 tactgaataa ccagcgtatt aatgatgttt tctgcggtgg tttgcgcttt ttgctgatcg
    16081 tcagctggca gcgtaatttg catggtcagc agttgattac ccacgttacc cagaataacg
    16141 gaagagtacg ccgtctggcc tttcgcggag ataatactgt ctaactgctg cattttgtga
    16201 cctttcagct caatggcttt attggttacc acttgcagct gcggatcgcg gctacgttgc
    16261 tgatcttcca gacgcttcgc caacaccgcc agatcttctt tcggatcatc gcccatgatg
    16321 acgatgactg ctttctgccc ggtggcgtcg gaccagacat gcatgttatt ggcctgcgtt
    16381 cccagcttac cgctctggtc ggtcatatcc gctggcagcg agaaacttaa cttgccatca
    16441 agcaggttga cgggattccc ggtagcgtta ctttccgcaa ccgaaccctg cgccgtagcg
    16501 ttagtgtctt tatcatcaca ggccgcaagc cccataacca gcaggccaat tccgacatat
    16561 ttaaccagat tgcgcattga cttcttcctt tcgataaacg gccataacgg ctcattcatc
    16621 catcttatca caactctgat aacgaacctt taactcgcct gcaaagcgtt gatttcggat
    16681 ttatctgcca gtcttttcaa cagcatattg agtaatacgc catacattgg caggaagaaa
    16741 acgatactga ttaacacttt gaaacagtaa tcgaccagcg cgatttccat ccagtgttca
    16801 gccataaagg catccgggct acgccagaag gcaatgaaga aaaaggccag cgtgtcgctg
    16861 acgttaccga acagtgtgga cgctgtcggt gccagccacc agcggcgact ctgacgcagg
    16921 cggttaaaaa cgtgcacgtc gaggatttgc cccagcgcgt aggccatgaa actggcggtg
    16981 gcgatacggg cgacaaacag gttgaagtgg gcgagtgcgc cgaatccctg ccaggaaccc
    17041 atatagaata gcgacgagat gacgtaggag attaataacg cagggatcat taccgcgaag
    17101 ataatgcgtc gggccagcgg tgcgccaaaa atacgcacgg tcaggtcggt agcaagaaaa
    17161 ataaacggaa agctaaacgc gccccaggtg gtatggaaac caaaaatgga gacgggaagc
    17221 tgaaccagat agttactgga ggtgatcacc agaagatgaa ataacgataa ccagaacaac
    17281 gccttatagc gttgagtttg cgagaaaacg ttcatattgt acctttttga ttaaccattg
    17341 gggtgaggga acccaatacg tacgacacgt ctgttacctt aacataataa cgtgcttacc
    17401 cttttttcga gccgccgcat gatactgctt tacgttgaca atgcaatggt taattttcac
    17461 gcaatcgtta acctggtttg cttacggact cacagggcgt aaactagcgc cgttttttta
    17521 agtgatgaga agaaaatgac cgatctcttt tccagccctg accacacact cgacgcgctt
    17581 ggcctgcgct gcccggaacc ggtgatgatg gtgcgcaaaa ccgtgcgcaa tatgcagcct
    17641 ggcgaaacgt tgctgattat cgccgacgat ccggccacta cccgcgatat tcctgggttt
    17701 tgtaccttta tggaacacga actggttgcg aaagagacgg atggactgcc ttatcgttat
    17761 ttgattcgta aaggcggttg ataggggctg attggcttcg atgccgcctt ttcctcaccc
    17821 cctaaccctc tccccagagg ggcgagggga ccgatcgcgc tcaatgttgc gatcggtttg
    17881 ccttatctcc tgcgcaacaa ccttaacgca ttcgccgtca ccagcaccgt cgcacccgta
    17941 tctgccagca ctgccagcca caacccggtc atccctaaaa gcgtggtgac gaggaagatc
    18001 cctttcagtc ccagcgcaat ggtgatgttc tggcggatat tggcgtgagt ggcgcgtgcc
    18061 agttcaatca tttgcaccag gccgcgcagg tggttatggg ttaatgctgc gtcggcggtt
    18121 tccagcgcca cgtctgtgcc gctacccata gcaatcccga tggcggcagc tttcatcgct
    18181 ggcgcgtcgt taataccatc accgaccatc gccaggggcg catgttgatt cagcttggtc
    18241 accgctttga ctttatcttc cggcaatagg cccgctttaa actccagccc cagctccccg
    18301 gcaattgccg ccgctgcgcg tggattatcg ccggtgagga tcactccttt aacccccagc
    18361 gcgttcagtt cactgatggc agttgtagcg tcggcgcgca gggtatcctg caatgcaatg
    18421 atacccagca cgtcatcatt acgtactacc agcacaaccg tttgcccggc gctttccagt
    18481 tcattaatca aaccagcaaa tgcatcagcg ggatgtttcc cggcagcgca tatcaacacg
    18541 cgctcaccat taacctgcgc ttcaatgcca gacccgacca gtgcccgctg tgattcggcg
    18601 gtgggaatgg cgagttcagc aacctgtgct tcgcgtacga tggcttgcgc cagtggatgc
    18661 gtcgcgcctt gctcgaccgc cgccgccagt gtcagcagtt cagattcact aatacccttt
    18721 gccggatgaa tcgcggtaac gcgcggttta ccgacggtca gcgtaccggt tttatcaaac
    18781 gccacttggg taacacgacc tagttgttcc agcgccgcac cgcctttaat taacgcccca
    18841 cgacgcgctg ctgctgccag cccggaggtt atcgccgcag gcgttgagat aactaacgca
    18901 cacgggcaac caatcagcag cagcgtcaga cctttataaa tccactcctg ccagctggcg
    18961 gcaaacagca gcggcggcac tagcgttacc agcagggcga cggccataat cgctggcgta
    19021 taaatacggc tgaaacggtc gataaaccgc tcaatggggg cgcggcgctc ttcggcttct
    19081 tcgatcagtt tcagaatccg gtcaatggcg ctggcacccg gttctgacag cacttccagc
    19141 gttaccagac ggtctacgct ggtggctcct gcaggaactt tatcgcccgt cgcgcgctcc
    19201 accggaatgg attctccggt tagggcgctt tcatcaaaac tggcaaacgg tgagagcagt
    19261 ttaccgtcgg caggcaaacg cccacctgcg gcgacttcaa tcacatcgcc aggccgcagg
    19321 ctgttaatcg ccacctcttc ccgctcaccg ttacgcaggc gcgtggcggt ttctggtttc
    19381 agcgccatta acgcgctaac gccctgacgc gcgcggctgg cggcccagcc ttccagtcgt
    19441 tcaccaatca aaaacagcag caacaccatc gcagcttcag ccgttgcgcc aataaacagt
    19501 gcaccaatag cggctacgct cattaaggtt tcaatggcga agtagctgcc ggatttgatc
    19561 agccgtaatg cctgacgagc aatcgggtac agcccaacta gcgtggtcgc gataaacgcc
    19621 agttgcccga acggatgatt gaactgctcc agaccccagc tgattgccat catcacgata
    19681 agcgtaatca gcggcagatt ctctttcagg cgcgatgctt gtggctcgtc ggcggcctgc
    19741 tcatcgcgca gggaatagcc cgctttttgc accgcagatt caacctgggc acggatgtca
    19801 ttgtcggcat caaccaccag tttttcggtg gcgaacaaca cctgcacctg attcacgcct
    19861 gcaagctggc gcacggcatt ttctaccttg cgcgcacagg cggcgcagtc catgccgctg
    19921 actttccagc tatagcgggt gccggagacg ttttcagaga gcgttggcga gctggagcat
    19981 gcgccgtcgc agcaacagtc gttggcgttc tgtaccgtgg ttagcggttt gaacgcagca
    20041 aattgagggg ctttcttgcc gtgattgtca ggtgtcgaca tggcatcctc cggttaagtt
    20101 ttttctcatt aaccgaagga tacactctgg agtcgactcc agagtcaagt tttatcagag
    20161 atacagtgag cggacgatga ggaagtgacc ggcaaagtag caggccgcag caatcgcgtt
    20221 atccgcacgg aagcgtcggc gatagtggct ccccagccag acaaagttac tgataaacag
    20281 caacgaagcg ccaacaaacg cagagagcgc cggagcggtc ggacggaaga accacagttc
    20341 acctgccagc cacaccatca ccagcgtcat gccgataaac gtgcagatag gccaacggta
    20401 ctcttccagc cgcgtccaga taatcgccag tagcagcgca cccagaacca gcaacaccag
    20461 tggcagaggc cagaagaaag agagcgtcat ctgactggcg aaatagatgg tgtacagcag
    20521 gtgcgagagg aaaaacgcgc cgatggcgta catcagacgt tgacgtggca acagggttag
    20581 cgcatcgccc agcagtgagg cgcacagtcc cgccagcacc agatagctaa tggcgtcgaa
    20641 catcggtgct tgccaggcca gtaacagcag gagaagaagg gttaacggtt taaacaccca
    20701 gcgttgccag gttggcccac gatacgatgc atccacagat agccatgcgg aaagacagac
    20761 agcgataaac gaccaaagca tcttagttcc ttgaattcgt tattttttcg taagcagaat
    20821 gcctacggtc ttgcttccag tttagtggcg agggtggacg gatgacaaca ccataactgg
    20881 cagggtatgc ttaattccgc attttccgat gagggataaa gatgagtaaa ccacctcttt
    20941 tctttattgt tatcattggc ttaattgtcg tcgccgcatc gtttcgtttt atgcaacagc
    21001 gacgggaaaa agctgataat gatatggctc cgctccagca aaagctggtg gtggtgagca
    21061 acaagcggga aaaaccgatt aacgatcgcc gttcgcgcca gcaggaagtg actccggcag
    21121 gcaccagtat gcgctatgag gcaagcttca aaccgcaaag cggaggaatg gagcagacgt
    21181 ttcgcctcga cgcccagcag taccacgccc tgacagtggg cgataaaggt acgctgagct
    21241 ataaaggaac gcgctttgtt agctttgttg gcgaacaata aggttatttt ttaactttaa
    21301 atttcttctg ccagaccagc agttcaaaca cgccgaaaag gaaaatccgc accttttcgg
    21361 cggtggtcat ctgcgggcca tctttcggta aagtggattt caatagcgcc agctgcatac
    21421 catgcatcag cacggtaaaa atcagcgcca cgttaacgaa aatatttagc gggcgcggga
    21481 agggatgcac cagattgagg attaaaaatc cccaaacgca gagcattaac aaacgaccaa
    21541 tattaatcag catcactttc tccttgtgct tcgtgttgat acagccgata agccacctga
    21601 cccgccactt tttcccgatg taatgaccag tttgccggaa cagtgggcag accgttttcg
    21661 acttcgcttt cgacataaat cagggcttcg tcagccagcc agccgttatc ttccagtaaa
    21721 tttatcgtct cttctaacaa gccacggcgg aacggtggat cgacaaacac gatattatgc
    21781 ggtgtgcctt tttgcgccag gaatgacatt gcgttgctgt tcaccacgcg tgcattgcct
    21841 gcttttagtg tcgccagatt cttaattaac tgctgagaaa ccacgcgatc catctcaatc
    21901 aacgttgccc ccgcagcgta gcgcgataac gcttccagcc ccagcgcgcc gctcccggcg
    21961 aagcaatcca gacattgggc gtcaacaatg accggagcca gccagttaaa caacgtttcg
    22021 cgtacgcggt cggtggtggg gcgcagacct gggctatcag gaaccgggag tttacggcct
    22081 cgccactgcc cgccaataat gcggatttgg ccgctgccgg aatgattcgg ttttttcatg
    22141 ataaattgct caatccgcca gataacacat acttgcaggc ggtgatgtga attaagttaa
    22201 gtgatagact atttcatcat ttttttagct gctatgtaca tagcgttaac gctgtgccat
    22261 gaagcaacag cgaggagtgt agtcgcaaat ggcgaaagaa aaaagacgtg gctttttttc
    22321 ctggctgggc tttggtcaaa aagagcagac cccggaaaaa gagacagaag ttcagaatga
    22381 acaaccggtt gtagaagaaa tcgttcaggc gcaagagcct gcgaaggctt ctgaacaagc
    22441 cgttgaagag cagccgcagg cgcatactga agccgaggcg gaaacttttg ctgccgacgt
    22501 tgtggaagtc actgaacagg ttgttgaaag tgaaaaagcg cagcctgaag cggaagttgt
    22561 tgcacagccg gaaccggtcg tagaagaaac gccggagcca gtggctatcg aacgtgaaga
    22621 gctgccgttg ccggaagacg tcaacgccga agaggtttcg tcagaagagt ggcaggctga
    22681 agcggaaacc gtagagattg tcgaagcggc agaagaagaa gcggctaaag aagaaatcac
    22741 cgacgaagag ctggaagcac aggcgctg
//
LOCUS       DAJTSI010000058        22412 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000058 DAJTSI010000000
VERSION     DAJTSI010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.058"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            82..2682
                     /locus_tag="OO327_002011"
     CDS             82..2682
                     /locus_tag="OO327_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HCR6018558.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            2673..3656
                     /locus_tag="OO327_002012"
     CDS             2673..3656
                     /locus_tag="OO327_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6018559.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQHNIISPAGGTLPLSIPDDADSIRE"
     gene            3754..4294
                     /locus_tag="OO327_002013"
                     /pseudo
     CDS             3754..4294
                     /locus_tag="OO327_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4302..4817
                     /locus_tag="OO327_002014"
     CDS             4302..4817
                     /locus_tag="OO327_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6018560.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4783..5520
                     /locus_tag="OO327_002015"
     CDS             4783..5520
                     /locus_tag="OO327_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR6018561.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5631..6641
                     /gene="pyrD"
                     /locus_tag="OO327_002016"
     CDS             5631..6641
                     /gene="pyrD"
                     /locus_tag="OO327_002016"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR6018562.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6815..7357
                     /gene="zapC"
                     /locus_tag="OO327_002017"
     CDS             6815..7357
                     /gene="zapC"
                     /locus_tag="OO327_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR6018563.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="OO327_002018"
     CDS             complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="OO327_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR6018564.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8707..10815
                     /gene="rlmKL"
                     /locus_tag="OO327_002019"
     CDS             8707..10815
                     /gene="rlmKL"
                     /locus_tag="OO327_002019"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR6018565.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10827..12734
                     /locus_tag="OO327_002020"
     CDS             10827..12734
                     /locus_tag="OO327_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6018566.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12864..14117
                     /gene="pqiA"
                     /locus_tag="OO327_002021"
     CDS             12864..14117
                     /gene="pqiA"
                     /locus_tag="OO327_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR6018567.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14122..15762
                     /gene="pqiB"
                     /locus_tag="OO327_002022"
     CDS             14122..15762
                     /gene="pqiB"
                     /locus_tag="OO327_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR6018568.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15759..16322
                     /gene="pqiC"
                     /locus_tag="OO327_002023"
     CDS             15759..16322
                     /gene="pqiC"
                     /locus_tag="OO327_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR6018569.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16578..16745
                     /gene="rmf"
                     /locus_tag="OO327_002024"
     CDS             16578..16745
                     /gene="rmf"
                     /locus_tag="OO327_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR6018570.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="OO327_002025"
     CDS             complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="OO327_002025"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR6018571.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17402..19162)
                     /locus_tag="OO327_002026"
     CDS             complement(17402..19162)
                     /locus_tag="OO327_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR6018572.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19347..19799
                     /gene="matP"
                     /locus_tag="OO327_002027"
     CDS             19347..19799
                     /gene="matP"
                     /locus_tag="OO327_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR6018573.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="OO327_002028"
     CDS             complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="OO327_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR6018574.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="OO327_002029"
     CDS             complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="OO327_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR6018575.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22005..>22412
                     /gene="sxy"
                     /locus_tag="OO327_002030"
     CDS             22005..>22412
                     /gene="sxy"
                     /locus_tag="OO327_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCR6018576.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEV"
BASE COUNT         5721 a         5258 c         5873 g         5560 t
ORIGIN      
        1 tatcgaacca tcaatgacta cggggcggaa acgccgcaac tcaactgtaa atcgtaagcc
       61 gtcttcagtt aagagagcga gatgtataga actcatcgac aacacagcct gttaagctct
      121 ggtggagtgc catcgtttat tggtgggctg gtggtgtttg tgtcggcagc gttcaatgca
      181 caagctgaaa cctggttcga tcctgctttt ttcaaagatg atccctcaat ggtggccgat
      241 ttgtctcgtt tcgaaaaagg acaaaaaata acgccagggg tttatcgtgt cgatattgtt
      301 ctgaatcaga caattgtaga tacgcgcaac gtcaattttg ttgagataac gccagagaag
      361 gggattgccg cctgtttgac gactgaaagc ctggatgcaa tgggtgttaa tactgatgcg
      421 tttccggctt ttaaacaact ggacaaacaa gtgtgtgtgc cattggcgga gattattccg
      481 gatgccagcg taacgtttaa tgtgaataaa ctccgtctgg aaatttcagt accgcaaatc
      541 gccatcaaaa gtaatgcccg tggttatgtc ccccctgaac gttgggatga agggatcaac
      601 gcgctattac tgggatattc gtttagcggg gctaacagta ttcatagcag cgcagacagt
      661 gattctggcg acagctattt tctgaattta aacagtggcg ttaatttagg cccatggaga
      721 ttacgcaaca attcaacatg gagtcgcagt agtggccaaa ccgcagaatg gaagaatctc
      781 agcagctatt tgcagcgggc ggttattcca ctaaaaggcg aactgaccgt aggtgatgat
      841 tatactgcag gcgatttttt cgatagtgtc agctttcgtg gtgtgcagct ggcgtcagat
      901 gacaacatgc tgccagacag cctgaaaggg tttgcgcctg tggtgcgtgg tatcgccaaa
      961 agcaatgccc agataacgat taagcaaaat ggttacacca tttaccaaac ttatgtatcg
     1021 cctggtgctt ttgaaattag tgatctctat tccacgtcgt cgagcggtga tttgttagtt
     1081 gaaatcaaag aagcggacgg cagcgtcaat agctacagcg taccgttttc cagcgtgcca
     1141 ttactccagc gtcaggggcg aatcaaatac gcggtgacac tggcgaaata cagaaccaat
     1201 agtaatgaac agcaggagag caaatttgcc cagaccacgt tgcagtgggg cggaccgtgg
     1261 ggaacgacat ggtatggtgg tggacaatat gctgaatatt accgtgccgc catgtttggt
     1321 ctgggattta accttggcga tttcggagca atttcgttcg atgcgaccca ggcgaagagt
     1381 acgctggcag accaaagcga acataaaggt cagtcatatc gttttctgta tgccaaaacg
     1441 ctcaaccaat tgggcactaa ttttcaattg atgggctatc gctattcgac gtcgggtttc
     1501 tacacccttt ccgacaccat gtataaacat atggatggct acgaatttaa tgacggtgat
     1561 gatgaagata cgccgatgtg gtcgcgttat tacaatttgt tttacaccaa acgtggcaaa
     1621 ctgcaggtca atatctccca gcaattaggc gagtacggtt cgttttattt aagtggtagc
     1681 cagcaaactt actggcatac cgatcaacag gatcggctat tacagtttgg ctacaacacg
     1741 caaattaaag atctctcgct gggggtttcc tggaactaca gtaagtcccg tggtcaacct
     1801 gatgctgatc aggtgtttgc actaaatttt tccctgccgc tcaatctgtt gctccccaga
     1861 agtaatgata gctataccag gaaaaaaaat tacgcctgga tgacctctaa caccagtatc
     1921 gataacgaag ggcacattac acaaaacctg ggtttaacgg agacactact cgatgacggt
     1981 aacctgagct acagcgtgca acagggatat aacagcgagg ggaaaacggc taatggtagc
     2041 gccagcatgg actacaaagg ggcgtttgca gatgcccgag tgggctacaa ctacagcgat
     2101 aacggcagtc aacaacaact gaactacgct ctttcaggca gtttagttgc ccattcacag
     2161 ggcattaccc tggggcaatc gctgggggaa actaacgtgc tgattgcagc accaggcgca
     2221 gagaatactc gtgtggcgaa cagcaccggg ctgaaaactg actggcgcgg atataccgtt
     2281 gtcccctatg ccacttctta tcgggaaaat cgaatcgcac ttgatgcggc atcgttaaaa
     2341 cgtaacgtgg atcttgaaaa tgcagtagtc aacgtggttc ccaccaaagg ggcgttggtt
     2401 ctggcggagt tcaatgccca tgcgggagca agggtattaa tgaaaacatc aaagcagggt
     2461 ataccgctgc gttttggcgc gatagcgacg ctggacggca tacagactaa tagcggcatt
     2521 attgatgatg atggctcgct ctatatgtcc ggtttgcccg ctcagggagc gataactgta
     2581 cgctggggcg aagctcccga tcaaatttgt catatcagtt accagcttac cgaacaacaa
     2641 attaactctg cgattacgcg aatggatgcc atatgcagat aatctttgga gaaaaatgcg
     2701 tgttattact acgactattt tttgccgccg tcttaatgct atggtgcgct caaactgctg
     2761 cttatatcgg gcagtgtcat accactcagg ggaatccgta tattggcgtc aattttggcg
     2821 ttaaaactct ggatgaagaa gaaaatacgg ctggagtagt taaagacaaa ttttatcagt
     2881 ggaacgaatc gaatgattat tatgtttcct gtgattgcga taaagacaat gtcagaagtg
     2941 gccgatgggc attcgccgcg gattcaccgt tagtctattt aggcgacaac tggtacaaaa
     3001 ttaatgacta tcttgccgcc aaagttttat tgcaggttaa aggcagttct cctactgcgg
     3061 ttcctttcga aaacgtgggc acaggggcag atacacgatg gcatatttgc gatcccggcg
     3121 gtcaacgttt aggtggccag ggggctagcg gtaatagcgg tagcttttcc ctgaaaatat
     3181 tgcagccgtt cgttggttcg gtcgtcattc ctcctatggc gctggcgcga ttatttgaat
     3241 gctacaacat acccgcaggt gattcctgca cgactactgg tacaccggtt ttagtgtatt
     3301 acctgtctgg tacgatcaat tcacttggct catgttccgt caatgccgga gaaacaatcg
     3361 aggttgatct gggcgacgta tttgcggcta actttcgtgt tgtagggcat aagcctcttg
     3421 gggccagaac ggcagaactt gcaattccgg tcaggtgtaa cacgggaaac gcagggttag
     3481 ttaacgtcaa tctgagtctg acggcaacct cagaccccag ctatccccag gcgattaaga
     3541 cgtcacgtcc tggcgtgggc gtggtggtga ccgatagcca gcacaacatt atttcccctg
     3601 ctggtgggac actaccgctc tctattcctg atgatgcaga cagtatccgc gaatgaatgt
     3661 ctatccagtc agcacgacaa gcgtaccacc agaaaccggg cgatttgaag ccacggcaac
     3721 ggtgagaata aattttgatt aacgcgtgaa cgtatgaaga aaaaaacgat atatcagtgc
     3781 gttattttgt tctttagcct tcttaacatc catgtcggga tggctgggcc tgaacaagtc
     3841 agtatgcata tttatgggaa tgtggtcgat cagggctgtg atgtcgccac caaaagtgca
     3901 ttacaaaata ttcatattgg tgattttaat atcagtgatt ttcaggccgc gaataccgta
     3961 agcactgctg ctgatttgaa tattgatatc accggttgtg ccgctggtat tactggcgcg
     4021 gacgtccttt ttagcggcga ggctgacacc cttgcgccga cactgctcaa actaactgac
     4081 acaggcggaa gcggtggtat ggcaacgggg attgccgtgc attcttgatg cgcaaagtca
     4141 gcaagaaatc ccgctcaatc aggtccagcc tcttacgccc ttaaaagccg gggataacac
     4201 actcaaatat caacttcgtt ataagtccac aaaggcggga gcaacgggcg gtaatgcgac
     4261 ggcggttctc tattttgatc tggtttacca gtgaaggagt gatgttgaaa cggataatct
     4321 ggattctgtt cttactggga ttaacggggg gctgtgagct atttgcccat gatggcacgg
     4381 tcaacattag cggatcgttt cgccgtaata catgcgtgct ggcacaggat agcaagcaaa
     4441 tcaatgtgca gttaggcgat gtctcgctga cacgtttttt tcatgggaat tatggcccgg
     4501 agaaatcttt catcattaat ttgcaggatt gcggaacaga tgtcagcacg gttgatgtca
     4561 ccttttcagg aacccccgac ggagtgcaga gcgaaatgct gtccattgaa agtggtacgg
     4621 atgcagccag tgggctggca attgcgattc tggatgatgc gaagatattg attccgctta
     4681 accaggcgag taaagactac agcctacata gcggtaaggt gccattaact ttttatgcgc
     4741 aattgcgacc tgtcaatagc gatgtgcagt cgggtaaagt gaatgccagc gcgacatttg
     4801 tacttcatta tgactaatac gtggaacaga ttagcgctct tgattttcgc cgttttatcg
     4861 ctgctggtgg cgggtgaatt gcaggcagga gtggtggtcg gcggaacgcg atttatcttt
     4921 ccggcagaca gagaatcgat atctatttta ctgactaata ccagtcagga atcttggctt
     4981 attaacagca aaatcaatcg cccaacgcgt tgggccgggg gggaagcgtc gacagtgcca
     5041 gcactattac tggccgctcc gccacttatt ctcctgaagc ccggtactac aggcacgttg
     5101 cgcttgctga gaacggaaag cgacatcttg cctgtggatc gcgaaacgct atttgagtta
     5161 agcattgcca gcgtgccatc cggcaaagtt gaaaatcaga gcgtaaaagt ggcgatgcgc
     5221 tcggtattta aactgttctg gcgacccgaa ggtttgccgg gcgacccgct ggaagcttat
     5281 caacaattac gctggacacg gaattcgcag ggtgtacaac tcactaaccc aacgccttac
     5341 tacattaacc tgattcaggt gagcgtaaat ggtaaagcgt taagtaatgc gggagtcgtg
     5401 ccgcctaaaa gccagcgtca gacaaactgg tgtcaggtaa tagcaccgtg tcatgtcgcc
     5461 tggcggacga taaatgatta cggcgggttg agtgcaaaga aggagcaaaa tctgccctga
     5521 aacaggttct gaaaacgttt gcgttttttt tgccgcaggt caattccctt ttggtccgaa
     5581 ctcgcacata atacgccccc ggtttgcaca ccgggaatcc aggagagttc atgtactacc
     5641 ccttcgttcg taaagccctt ttccagctcg atccagagcg cgctcatgag tttacttttc
     5701 agcaattacg ccgcattacc ggaacaccgt ttgaagcact ggtgcggcag aaagtgcctg
     5761 cgaaacctgt taactgcatg ggcctgacgt ttaaaaatcc gcttggtctg gcagccggtc
     5821 ttgataaaga cggggagtgc attgatgcgt taggcgcgat gggatttgga tcgatcgaga
     5881 tcggtaccgt cacgccacgt ccacagcctg gtaatgacaa gccgcgtctc tttcgtctgg
     5941 tagatgccga aggtttgatc aaccgtatgg gctttaataa tcttggcgtt gataacctcg
     6001 tagagaacgt aaaaaaggcc cattatgacg gcgtcttggg tattaatatc ggcaaaaata
     6061 aagatacgcc agtggagcag ggcaaagatg actatctgat ttgtatggaa aaaatctatg
     6121 cctatgcggg atatatcgcc atcaatattt catcgccgaa taccccagga ttacgcacgc
     6181 tgcaatatgg tgaagcgctg gatgatctct taaccgcgat taaaaataag caaaatgatt
     6241 tgcaagcgat gcaccataaa tatgtgccga tcgcagtgaa gatcgcgccg gatctttctg
     6301 aagacgaatt gatccaggtt gccgatagtt tagttcgcca taatattgat ggcgttattg
     6361 caaccaatac cactctcgat cgttctctgg ttcagggaat gaaaaattgc gatcaaaccg
     6421 gtggcttaag tggtcgtccg cttcagttaa aaagcacaga aattattcgc cgcttgtcac
     6481 tagaattaaa cggtcgctta ccgatcatcg gtgttggcgg catcgactcg gttatcgctg
     6541 cgcgtgaaaa gattgctgcg ggtgcctcac tggtgcaaat ttattctggt tttattttta
     6601 aaggtccgcc gctgattaaa gaaatcgtta cccatatcta attatttctt cgaattcctc
     6661 tttataagat aatcagggct ttattttcag acctggttgt tttatattca tcgttgttgc
     6721 ttatttagac attttgtact tttattgttg aggttattaa gcgaagcgac aatggattgt
     6781 gttgctgcgg ttttataggg taggggagag gcagatgcga attaaaccag acgataactg
     6841 gcgttggtat tacgatgaag agcacgatcg aatgatgctc gatttagcca atggtatgct
     6901 atttcgctca cgttttgcgc gcaaaatgtt gactccagat gccttttctc ccgcaggctt
     6961 ttgtgttgat gatgccgcgc tctatttctc ttttgaagaa aagtgccgcg attttaattt
     7021 atctaaagac caaaaagccg aactggtgct gaatgcactg gtagcaattc gttatctcaa
     7081 accgcaaatg ccgaaaagct ggcattttgt ctcccatggt gaaatgtggg tgcccatgcc
     7141 gggcgatgcg gcctgcgttt ggttgagcga tactcacgag caggtcaatt tgctggtcgt
     7201 tgaatctggc gaaaatgccg cgctatgcct gctggcacaa ccctgcgttg ttattgcggg
     7261 tcgcgcgatg cagttgggcg acgccattaa aatcatgaac gacaggctga aaccgcaggt
     7321 gaatgttgac agcttcagcc tcgaacaggc agtctaacgc gtcagcttaa gggcagtctt
     7381 cggtacacag ctacagcaaa gaatggtgcc atcatcgccc attgctgatt ttttcagcgg
     7441 tgtgacttcg ccttctaaaa gctgaacacg gcaacttcca caaatgcccg cgcggcaaga
     7501 ataagggata cgaattccct gattttctaa ttgctccagc aacacttgtt ggttatttcc
     7561 acgaaatgcc tgtccctgcc agtcaatatc tacatttgcg tccggttgtt gcgtgatgtt
     7621 ggcggtatca tcagcggcag ctgcgccgta aatttttgcc ggagccgttg ccagaatttc
     7681 cacctcatcg ccaacgcgaa tcacgccgct attacgggca attaaattct gaccaaaatc
     7741 gacatcgccg ttatcctggg cagtgcggaa agattgcaat gtttttaatg gttcgcctgc
     7801 cggatgtttt tgcccttttt ctgggctgac ggtggtgaaa atacagcggc tacaaggttt
     7861 aaccacatca aacaccacat caccaatgcg aatcactttc cagctatctt cttcccaggc
     7921 tgacgcgccg gaaaccacca gattggggcg gaattgctcc atttttacac tggccggaca
     7981 acgttgttgg agatcacgta acgaggcttc gttagcaaga aggtaaggat agccatcagc
     8041 aaatgacaga ggtacagtgt tgtggcgttt cacgcgccgg gtcatttgtg gccccaccca
     8101 gcgtaattgc acttcgcggg agaaaaatcc acttagccat ttgttgatcg cgtctggcgc
     8161 aattcgcgcg gtaaaatgtg tgccccaaac ttcggttggt gcgtcttgtg tggcgaaatc
     8221 agcaaaacga acatatgcgc tactgccatc tggtgcggtg agatgcaagc catcatgcac
     8281 aggtgaagga gtaaaccgta ccatctgggg aaactggcga gcggtaataa acgtaccgtc
     8341 aggttccgtg atcataaaga tgcgatcgaa ggccagacca ctgacatcag ccagagcatg
     8401 tgtaagacca atgccgcgca tcgatttaac aggatgaata aaaagccggg ttaatgtcgc
     8461 cacggtgcgg tcctcaaatg aaaataagcc ctcaacttta tgacatgacg cgcttattag
     8521 ctataatgcg caacaatttt cttagcgcct gtcccgttag gctattttat ttgtcagttt
     8581 ggccctgggt tgtgctcgaa atcctcacgt acttgtgtac gctccggttt ctccgcgctc
     8641 tccatgtcca gactgactgc aacaattacg cctactgcgg taggctctta gagtaaaagt
     8701 gacgatatga attctctgtt tgccagtacg gcccgtgggc tggaagagct gttaaaaact
     8761 gaactggaaa atctgggggc cgttgaatgc caggtggttc agggtggggt ccatttcaag
     8821 ggcgacacac ggcttgttta ccagagcctg atgtggagcc gcctggcctc gcgtattatg
     8881 ttgccgctgg gcgagtgtaa ggtttacagc gatttagacc tctatctcgg tgttcaggcg
     8941 atcaactgga cagagatgtt taatcctggc gcgaccttcg ctgtccactt cagtggtttg
     9001 aatgacacca tccgcaacag tcagtacggc gcgatgaaag tgaaagacgc gatcgtcgat
     9061 gctttcacac ggaaaaatct gccgcgtcca aatgttgatc gcgatgcgcc ggatatccgc
     9121 gttaacgtct ggctgcataa agaaaccgcc agtatcgctc ttgatctgag tggtgatggt
     9181 ttacatctgc gtggctatcg cgatcgtgct ggtattgcgc cgatcaaaga aaccctggca
     9241 gccgctattg tgatgcgatc cggctggcag tcaggaacac cgctgctcga tccgatgtgt
     9301 ggttccggta cgttgctgat tgaagcggcg atgctggcga ccgatcgcgc accaggcttg
     9361 caccgtgggc gttggggctt tagcggctgg gcgcagcatg atgaagctat ctggcaggaa
     9421 gtgaaagcgg aagcgcaaac tcgcgcccgt aaaggcctag ctgagtatag ctctcatttt
     9481 tacggttcgg acagcgacgc acgggtgatt caacgtgcgc gcactaacgc ccgtcttgcg
     9541 gggattggtg aactgatcac ctttgaggtg aaagatgtcg cgcaactgac caatccgctg
     9601 ccgaaagggc cgtacggtac agtgttgagc aacccgccat acggtgaacg tctggacagc
     9661 gaaccggcgc tgattgcgct gcatagtctg ctgggccgaa tcatgaaaaa ccagttcggt
     9721 ggctggaatc tctctttgtt tagtgcctcg ccggatctgc taagctgcct gcagctgcgt
     9781 gcagacaaac agtacaaggc gaaaaacggc ccgctggact gcgtacagaa aaattaccac
     9841 gttgccgaaa gcacaccaga cagtaaaccg gcaatggtag cggaagatta cgccaaccgc
     9901 ctgcgtaaga acctcaaaaa attcgagaag tgggcacgcc aggaagggat tgaatgttac
     9961 cgcctgtatg acgccgatct gccggaatat aacgttgccg ttgaccgtta tgccgactgg
    10021 gtggtggtgc aggagtatgc gccgccaaaa actattgatg cgcacaaagc gcgtcagcgt
    10081 ctgttcgata ttatcgctgc aaccatttcg gtactgggga ttgcgccaaa caaactggtg
    10141 ctgaaaaccc gtgaacgcca taagggcaaa aaccagtacc agaaactggg cgagaagggc
    10201 gagtttcttg aagttaccga atataacgct cacttgtggg tgaacctgac ggattatctc
    10261 gatactggtc tgttcctcga tcaccgtatc gcccgccgta tgctcggtca gatgagcaaa
    10321 ggcaaagatt tcctcaacct gttttcttat accggcagcg ccaccgtgca tgcgggatta
    10381 ggcggtgcac gcagcaccac caccgtggat atgtcgcgta cttatctgga atgggcagaa
    10441 cgcaacctgc gtctgaatgg cctgactagg cgtgcgcatc gcctgattca ggccgattgc
    10501 ctggcgtggc tgcgtgaggc aaatgaacag ttcgatctga tctttatcga tccgccaacc
    10561 ttctctaact caaaacgaat ggaagatgcg tttgatgttc agcgcgatca tctggcgctg
    10621 atgaaagatt tgaaacgtct gctgcgtgca ggtgggacga tcatgttctc gaacaacaaa
    10681 cgtggcttcc gtatggatct cgacggcctg gcaaaactgg gactgaaagc acaagaaatt
    10741 acgcaaaaaa cgctctccca ggatttcgcc cgtaaccgcc agatccacaa ctgctggctg
    10801 attaccgcag cctgaaagga atagtaatgt cattaatcag tatgcatggc gcatggctgt
    10861 cgttcagcga cgcgccgctt ctcgataacg cagaactgca tatcgaagat aacgaacgtg
    10921 tttgtctggt gggccgcaac ggcgcaggaa aatcgacgtt aatgaaaatc ctcaaccgtg
    10981 aacaagggct ggatgacggt cgcattattt atgagcaaga tttgattgta gctcgtctgc
    11041 aacaggatcc gccgcgtaac gttgagggta gcgtttatga tttcgttgcc gaaggcattg
    11101 aagaacaagc ggaatatctg aaacgctatc acgatatttc gcgcctggtg atgaacgacc
    11161 cgagcgagaa aaatctcaac gaactggcga aggttcagga acagctggat caccacaacc
    11221 tgtggcagct ggaaaaccgc atcaacgaag tgctggcgca actggggtta gatcctaacg
    11281 ttgcgctgtc gtcgctttcc ggcggctggt tgcgtaaagc ggcattagga cgcgcgctgg
    11341 tgagcaatcc gcgcgtattg ctgctggacg aaccgactaa ccatctggat atcgaaacca
    11401 tcgactggct ggaggggttc cttaaaacct tcaatgggac gattattttc atctcccacg
    11461 accgttcgtt tatccgcaat atggcgacgc gcattgttga tctcgatcgc ggcaagctgg
    11521 tgacctatcc agggaattac gaccagtacc tgcaggaaaa agaagaagcc ctgcgcgtgg
    11581 aagaactaca aaatgccgag ttcgatctca aactggcgca ggaagaagtg tggattcgcc
    11641 aggggatcaa agcacgccgt acccgtaatg aaggccgcgt acgcgccctg aaagcgatgc
    11701 gtcgcgaacg tggtgaacgt cgcgaagtga tgggtaccgc aaagatgcag gtggaagagg
    11761 ccagccgctc cggtaagatc gttttcgaaa tggaagacgt ttgctaccag gttaacggta
    11821 agcaactggt gaaagatttt tctgcccagg ttctacgtgg cgacaaaatt gccctgattg
    11881 gtccgaatgg gtgcggcaaa accacgctgc tgaaactgat gctcggtcag cttcaagcgg
    11941 acagcgggcg tattcacgtt ggcaccaaac tggaagtggc ttatttcgat cagcaccgcg
    12001 cggaactgga tcccgataaa acggtgatgg ataaccttgc cgaaggtaag caagaggtga
    12061 tggttaatgg caagccacgc cacgtattgg gctatttgca ggactttctg ttccatccga
    12121 aacgggcgat gacgccagta cgtgcgtttt ctggcggtga gcggaaccgc ttgctgctgg
    12181 cgcgtttgtt cctcaaacca agcaacttat tgattcttga cgaaccgacc aacgatcttg
    12241 atgtcgaaac gctggaactg ctggaagaac tgatcgacag ctatcagggc acggtattgc
    12301 tggttagcca cgatcgtcag tttgtcgata acaccgttac agaatgctgg atcttcgaag
    12361 gcggcggtaa aattggtcgt tatgttggcg gttatcatga tgcccgtggt cagcaagaac
    12421 aatatgtggc actcaaacag cctgcggtga aaaaaagcga agaagccgcc gcgccaaaag
    12481 cagaaactgt aaaacgcagc agtagcaaac taagctataa attgcagcgc gaactggagc
    12541 agctaccgca attgctcgaa gatctggagg cgaagctgga agccctacag acgcaagtgg
    12601 cggatgcttc cttcttcagt cagccgcatg agcagacgca aaaagtgctt gctgatatgg
    12661 ctgctgcaga gcaggagctg gagcaagcct ttgaacgctg ggagtatctt gaagcgttaa
    12721 aaaatggtgg ctgatcgcaa aatagtcgat ttaaacagag acagctgaca acggtaaaat
    12781 tgcctgatga actgggttta tcaggcatga aaaccaacac tttgttagtc atctgtaaaa
    12841 tagcgcatca ttaaggagta ccaatgtgcg aacatcatca tgccgcgaag cacatcctgt
    12901 gctcgcagtg tgacatgctg gtggcgttac cgcgccttga gcatggtcag aaagcggcat
    12961 gtccccggtg tggcacaacg ttaaccgtgg cgtgggatgc ccctcggcag cgtcccaccg
    13021 cctatgcgtt ggctgcactg ttcatgctgt tgctgtccaa cttgtttcct tttgtgaata
    13081 tgaacgttgc aggagttacc agtgaaatta cattactgga aattcccggc gtgctttttt
    13141 ctgaggacta cgccagcctc ggcacctttt tcctgttgtt tgtgcaactg gttcccgcgt
    13201 tttgtctgat aaccattctg ttactggtga atcgcgcgga attaccggtc cgtttaaaag
    13261 agcaactggc acgggtgctt tttcaactca aaacctgggg aatggcggag attttcctcg
    13321 cgggtgtgct ggtcagtttc gttaaactga tggcttacgg cagcattggg gtaggcagca
    13381 gctttctccc ctggtgttta ttttgtgtcc tgcaactgcg cgcttttcag tgcgttgatc
    13441 gtcgctggtt atgggacgac atcgccccga tgccagaact gcgccagccg ctaaaaccag
    13501 gcgtcacggg gatacgtcag gggctgcgtt cgtgctcctg ttgtacggca atccttcctg
    13561 ctgatgaacc cgtgtgcccg cgttgtagta ccaaagggta cgttcggcgt agaaacagcc
    13621 tgcagtggac actcgcgctg cttgtaacgt ccatcatgct gtatcttccg gctaatattt
    13681 tgcccatcat ggtgacggat ttattaggct cgaagatgcc gtcgacgatt ctcgctgggg
    13741 tcattctgtt atggagcgaa ggctcttatc ccgtcgctgc ggtgatcttt ctggccagta
    13801 ttatggtgcc aacgttaaag atgatcgcca tcgcgtggct gtgttgggat gccaaagggc
    13861 atggcaagcg cgacagtgaa agaatgcatt tgatttatga agttgttgag tttgtaggcc
    13921 gctggtcgat gattgacgtt ttcgttatcg cggtgctctc ggcgctggtg cgtatgggag
    13981 gtttaatgag tatttatccg gcaatgggtg cattaatgtt tgctttagtc gtcataatga
    14041 caatgttttc tgctatgacg tttgacccgc gtttgtcgtg ggatcgtcaa cctgaatcag
    14101 agcatgagga gtcctgacag tatggaatct aataatgggg aagccaaaat ccagaaagtg
    14161 aagaactggt ctcccgtgtg gatatttcct atcgtcacgg cgctcattgg ggcctgggtt
    14221 cttttttatc attacagcca tcagggaccg gaagtgaccc tgatcaccgc gaatgcggaa
    14281 ggaattgaag gtggcaaaac caccattaaa agccgtagcg ttgacgtcgg cgtggttgaa
    14341 agcgccacac tggctgatga tttgacgcac gttgaaatca aagcgcggct gaattccggt
    14401 atggaaaaat tgctgcataa agacaccgtc ttttgggtgg tgaaaccgca gattggtcgc
    14461 gaagggatta gcggcctggg aacgctgctg tctggagttt atatcgaact gcagccaggc
    14521 gcgaaaggca gcaaaatgga taaatacgat ttgctggact caccaccgtt ggccccgcct
    14581 gatgcgaaag gtatccgtgt gattctcgat agcaaaaaag ccgggcagct ctcgccagga
    14641 gatccggtgc tgttccgtgg ctatcgggta ggttcggttg aaaccagcac cttcgatacg
    14701 caaaaacgca atatcagtta tcaactgttc atcaatgcac cttatgaccg actggtgacc
    14761 agcaatgttc gcttctggaa agatagtggc attgcggttg atctgacgtc agcggggatg
    14821 cgtgtggaga tgggctcatt gacaacgctg cttagtggcg gtgttagctt tgatgtgccg
    14881 gaaggtctgg atttagggca gccagtggca ccgaaaacag ctttcgtttt gtatgatgat
    14941 cagaagagca ttcaggattc gttgtacacc gatcacattg attatctgat gttctttaaa
    15001 gattcggtac gcggtctgca accgggagct ccggtagaat tccggggtat tcgcctgggt
    15061 accgtaagca aagtgccatt ctttgcgccg aatatgcgtc agacatttaa cgatgattac
    15121 cgtattccgg tactgattcg tatcgagcca gagcggctga aaatgcagct tggcgaaaat
    15181 gcggatgttg ttgagcacct tggcgaattg ttgaaacgtg gtttacgcgg atcgctgaaa
    15241 accggaaacc tggtcactgg tgcactgtat gttgatctcg atttctatcc aaatacgcct
    15301 gcaataaccg gtattcgtga atttaatggt tatcagatta tcccgactgt tagcggcggc
    15361 ttggcgcaaa tccagcaacg actgatggaa gcgttggata agatcaacaa actgccattg
    15421 aatccgatga ttgaacaggc aaccagtacg ctttctgaaa gtcagcgcac aatgaaaaac
    15481 ctgcaaacga cgctggatag catgaacaag atcctcgcca gccagtcgat gcagcagttg
    15541 ccgacggata tgcagtcaac gttgcgtgaa ttgaatcgca gcatgcaggg cttccagcct
    15601 ggctccgcag cctacaacaa gatggtggcg gatatgcagc gccttgatca ggtgttgcga
    15661 gaactgcaac cggtgctgaa aacgctcaat gagaagagta acgcgctggt atttgaagcg
    15721 aaggacaaaa aagatccaga gccgaagagg gcgaaacaat gaaaaagtgg ctagtgacga
    15781 ttgcagcact gtggctggcc ggatgcagct ccggcgaaat taataaaaac tattaccagt
    15841 tacctgtggt gcagagcggt acacaaagta ccgccagcca gggcaatcgt ctgttatggg
    15901 tagagcaggt cactgttcct gactatctgg cggggaatgg tgtggtttat caaaccagtg
    15961 atgtgaagta tgtgattgcc aacaacaact tgtgggccag cccgttggat caacagttgc
    16021 gcaacaccct ggttgccaac ctgagtacgc aactgcccgg ctgggtggtt gcctcccagc
    16081 ctctgggaag cgcccaggac acgctcaatg ttaccgtaac ggagtttaac ggtcgctatg
    16141 atggcaaggt cattgtcagt ggtgagtggc tgttgaacca ccagggacaa ctgatcaaac
    16201 gtccgttccg tctggaagga gtgcaaactc aggatggtta cgatgagatg gttaaagtgc
    16261 tggccggtgt ctggagtcag gaagccgctt ctattgcaca agagataaag cgtctacctt
    16321 aattataaag atttgtaaat ataaccgtct ccggtatgtt gcctgaggcg gtttttttgt
    16381 ttctaacgtg cggaaaaatt tgttcctctt cacatttttt gtacaaccga catgcccgtg
    16441 tagctcacaa atatgacagt ggcgtgaatt ttgcgcattg acggcagtta tgattcgcgg
    16501 tattgcttaa ctgtgattgc acatttagta atcactgttt tcttttccac cagaaaccag
    16561 tatgagggaa acgaggcatg aagagacaaa aacgagatcg cctggaacgg gcacatcaac
    16621 gtggttatca ggccggcatc gccggacgct caaaagaaat gtgtccctat cagacgctga
    16681 gtcaaaggtc acaatggctg ggaggctggc gagaagccat ggcggacagg gtagtaatgg
    16741 cctgattctg tctctttaaa aagaaacctc cgcattgcgg aggtttcgcc ttttgatact
    16801 ctgtctgatg ataatcagaa ggcagacgta tcctggaaca gaccgacttt caggtcgctg
    16861 gcggtataga tcagacgaca atcaaccagc acttcgccat ctgccagacc cataatcaga
    16921 cgacgattaa caatgcgttt aaagtgaata cggtaggtca cttttttcgc tgtcggcagt
    16981 acctgaccag tgaatttcac ttcgccaacg cccagcgcgc ggcctttacc ttcgccgccc
    17041 agccagccga ggtagaaccc taccagctgc cacattgcgt ccaggcccag gcatcccggc
    17101 ataaccggat cgccaatgaa gtggcatccg aagaaccaca gatccggatt gatatccagt
    17161 tctgcttcaa catacccttt gtcgaagtta ccacccgttt cggtcatttt gaccacacgg
    17221 tctatcatca gcatgttcgg tgctggcaat tgcgggcctt tagcgccaaa cagttcaccg
    17281 tgaccagagg caagaaggtc ttcttttgta taggattcgc gtttatctac catgttctct
    17341 gtaagcctta ttttattgaa gcacgcagga tagctaacac gtgtacgctg aacaagtccg
    17401 atcagttcgg aataaaccag ttcagccaac gtaatggcca tggaaaacgg tgacgtcctt
    17461 cctgttgcga tgcttgcgcg atacgttcct ggatggtttg catcagcgtc gtttggcctt
    17521 cgccatccca caccagattt aataataacg gcagtgcgtc agtcacatcg tctactgccc
    17581 agatggtgaa tttgccttct tctaccgctt tcaccagttc actgtgaaga cttaaatggc
    17641 gaacgttagc agtggggata atgacacctt gtttccccgt taactcacgt tgctggcaaa
    17701 tagcaaagaa gccttcgatt ttctcattta aaccaccgac cggctggacg cggccgaact
    17761 gatcgactga acctgtgata gcgatactct gattcactgg cacatcagcg agggcgctta
    17821 tcagggcgca gagttcagcc atcgaggcac tatcgccatc cacttcactg tatgactgct
    17881 caaatgtcag cgatgctgag aaggggatct gttgctcaag ctgtagttcc gacatcagga
    17941 acgcctgcat gatcatcatc cctttcgcat ggatattgcc gccaagctcc gctttgcgtt
    18001 cgatgtcggt gaattcacca tcgccaatat gcacaacgca gctaatgcga gaaggttcgc
    18061 caaaagcgcg tggatgaccc ggaaattcaa tgaccgaaag ggcgttaatt tgcccgatgc
    18121 gttcgccttc ggtttcaatc aggatttgct cctgaaggat ctcatcctgc atacgttcag
    18181 cgaggaaacc ttcgcgccat tcacgctgct gcagcattaa gtttagctgc tcgccggaga
    18241 aggtgtcgcc atcacacagg gaggcgacct ctttacactg gcggaggatc cactgcgggc
    18301 taagcggaag tgtttcttgt tcaccggtgt agcgtgctgc ttcgcggata agtaccggcc
    18361 aagcatccgc tcccggggca ggtaagtgat tatgtctggc ggtaaatgtc acccagcgac
    18421 accactggct tactgactcc gcatcgacaa tctgcagagt atcttcaaat tcgctataaa
    18481 tagcctgctc tgaaagctct ggctccatct cctggaaatc agccaatgat tcgcgttcgc
    18541 ctaccagaat gactttcagc ttcaatggca ttgaaggcac agagacgggg agagggcgcg
    18601 actcatcaaa cgcaacccag tcaaaacgct cgcggttaac gatatttttc agccgcatcc
    18661 acagcagagg ttgcgccagc agtgtacgca aagagatgat gagaataccg ccatttgcct
    18721 gatgcaccag accaggctgc agggtaatgt cgccattaaa ctggcgcagg cagccaaaga
    18781 gttgctccgc ttctacccag tcggcagcga caacttgcgt taaagtcgca aaattatcat
    18841 ctgcactcac tgcgtgacgt aagcggatgg agtggccgga aacctcatag tgatcgccca
    18901 ccagttggcc tgcatcgctt tgtagcgtac gcgcggcatc ggcaataaga ttcagatact
    18961 cagactcttc cggggcctta gccagcataa aagaggagga tgctcgcgta tgcagcaact
    19021 gctccagcgc aaattgcagt cgcggttgag tatcactgaa taaaggatcg ttttcgtcaa
    19081 tcaaatgtgg ctgagcaaat atttcctgat agctatcggt atcaggaacc aggtcacgcc
    19141 atgcaagttt cgtaatggtc aaagttgatg ttttttagtc tgttgtcaaa gccgcgatta
    19201 taccgtaacc ggcactacag cacacgtaga aagcaccgac aatactcctg gctgggcgtt
    19261 aaagctcaca ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag
    19321 agttacacgg taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg
    19381 gttggaaatg gaagtatctg gtgaagaagc atcgcgaagg ggagttaatt acccgttaca
    19441 tagaagccag tgccgcccag gaagccgtcg atgtgttgct ctcgctcgaa aatgaacccg
    19501 tgctggtaaa tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc
    19561 agaccattcg ggccagacgg aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa
    19621 aatcgatcga tctggaattt attgtctggc aacgtctggc tggtcttgca cagcgtcgcg
    19681 gtaaaacgct ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat
    19741 acgcgaataa aatgtcttct cttaagcagg atctgcaggc attgctgggt aaggaataac
    19801 tgacgaaagt cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc
    19861 taccagacga taacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg
    19921 atctctacgc gacgatccgg agccaagcag tcgatcagtg cagcacgctg tttcacgttg
    19981 tcacaggtgt tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct
    20041 gccgggatac ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga
    20101 ccctggttgt aagcgtcaga accgatgcgg tcagtgtaac ccagaacaac tacggaaccg
    20161 tctttcggat ctaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc
    20221 ggtttcaggg ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg
    20281 gtctgtactt ccggtgccgg agctggagca actaccggag ctgcttcgcc ctgaccgaaa
    20341 cggtaggaaa cacccaggct cagcagaccg ttgtccggac gagtaccgat ggtgtttgcg
    20401 tcaccgatgt tgttggtcca ctggtattcc agacgggtag cgatttcagg agtgatcgcg
    20461 tactcaacac cacctgcgaa aaccggagaa acgccagtgt cgtggtcttt aaaggatgcg
    20521 ccaccaggta cgttagcctt ggtgtctgca cgccatacca taccacccag acgagtgtag
    20581 atgtccagat cgtcagtgat tgggtaaccc agtttagcgg tcagctgaac gccctgagct
    20641 ttgtatgcgc cgttgatgtt gtcgcctttg tacggcatac gacctaacca gtcgtaaccc
    20701 atttcaaaac caacatacgg gttaacctgg taaccaccaa aagcacctgc acccagttgg
    20761 ttttcgtggg tcggaccatt gttaggaata aaaccagtgt catggtactg ggaccagccc
    20821 agtttagcac cagtgtacca ggtgttatct ttcggagcgg cctgcgctac ggtagcgaaa
    20881 ccagccagtg ccactgcaat cgcgatagct gtctttttca ttttttgcgc ctcgttatca
    20941 tccaaaatac gccatgaata tctccaacga gataacacgg ttaaatcctt caccggggga
    21001 tctgctcaat attaactcta ccgatatctt cggcttatgc cgagcacccc tggcgatgta
    21061 aagtctacaa cgtagttgaa aacttacaag tgtgaactcc gtcaggcata tgaaaaaaaa
    21121 gtcttgtata aggtatgttt aatctttttg tcagcgacaa tttacagaaa aaaaacgcgg
    21181 aaaccgcttc agacaagcct tcgcaaggaa aattagtcac gactgaaagc attggctggg
    21241 cgacaaaaaa agttctagga ttaatcctaa atttacttaa tgatacaaat tagagtgaat
    21301 ttttagcccg gaaagttgtc tcgttgtgtg agaggatgcg cttaccggac gcataataaa
    21361 ccccatagcg ttaccttcat ttgccgcatc aacaagttca gcatgctctt cttcagtcaa
    21421 atcatctgtc aaccaaccga tcaccacact gtaattgccc gtgcgtaaag cgcgaaccat
    21481 tgactccact gtgtggcaag gggagagctg gctaatctgc attactttcg ttaagggtag
    21541 cccagatgcc tgaacccatt cccgactcag tttttgttgc ggtgttaacc agagttgcca
    21601 gcgcgattgc tgaccgagtt gctgtaacaa tggcaacagt agaagttgcg tcatcatggg
    21661 ctgatcttcg cgatagacaa cttcactgat aagcccggct gtagtgtttt ccgtagagac
    21721 acgcgcaatt ttacttgctg cggatgagaa cgacgaagaa cgatgtgcat agcctgaagt
    21781 gtacataatc aatccagccc ctgtgagtta ctgtatggat gtgcagtaca tccagtgaca
    21841 acaaagatca accctatttt cggaaagagc ctcgcaaatt ttgtcgttgg tgacgggaaa
    21901 acataaatta atcttgcccc ttaagaataa gttgcctatt ttcgtagtta acggatccgt
    21961 taatgtgaat cattctttta tgttatgatt ttaaaaggaa ttttatgaaa agcctctcct
    22021 ataagcggat ctataaatca caagaatacc tggcaacgtt gggcacaatt gaataccgat
    22081 cattgtttgg cagttacagc ctgaccgttg acgacacggt gtttgcgatg gtttctgatg
    22141 gtgagttgta tcttcgggct tgtgagcaaa gtgcacagta ctgtgtaaaa catccgcctg
    22201 tctggctgac atataaaaag tgtggccgat ccgttaccct caattactat cgggttgatg
    22261 aaagtctatg gcgaaatcaa ctgaagctgg tgcgtctgtc gaaatattct ctcgatgcag
    22321 cgctgaaaga gaaaagcacg cgcaataccc gggaaagact gaaagatttg cccaatatgt
    22381 cttttcatct ggaagcgatt ctcggggagg tg
//
LOCUS       DAJTSI010000059        22281 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000059 DAJTSI010000000
VERSION     DAJTSI010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.059"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1028)
                     /locus_tag="OO327_002031"
     CDS             complement(<1..1028)
                     /locus_tag="OO327_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6018577.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEA"
     gene            complement(1130..2110)
                     /locus_tag="OO327_002032"
     CDS             complement(1130..2110)
                     /locus_tag="OO327_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR6018578.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPCYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2118..2828)
                     /locus_tag="OO327_002033"
     CDS             complement(2118..2828)
                     /locus_tag="OO327_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCR6018579.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3045..4868)
                     /gene="typA"
                     /locus_tag="OO327_002034"
     CDS             complement(3045..4868)
                     /gene="typA"
                     /locus_tag="OO327_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR6018580.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5241..6650
                     /gene="glnA"
                     /locus_tag="OO327_002035"
     CDS             5241..6650
                     /gene="glnA"
                     /locus_tag="OO327_002035"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR6018581.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6823..7872
                     /gene="glnL"
                     /locus_tag="OO327_002036"
     CDS             6823..7872
                     /gene="glnL"
                     /locus_tag="OO327_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR6018582.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7884..9293
                     /gene="glnG"
                     /locus_tag="OO327_002037"
     CDS             7884..9293
                     /gene="glnG"
                     /locus_tag="OO327_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR6018583.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9405..9515
                     /locus_tag="OO327_002038"
     CDS             9405..9515
                     /locus_tag="OO327_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR6018584.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9744..11117)
                     /gene="hemN"
                     /locus_tag="OO327_002039"
     CDS             complement(9744..11117)
                     /gene="hemN"
                     /locus_tag="OO327_002039"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR6018585.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11306..11815)
                     /gene="yihI"
                     /locus_tag="OO327_002040"
     CDS             complement(11306..11815)
                     /gene="yihI"
                     /locus_tag="OO327_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR6018586.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDECLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            12397..13029
                     /locus_tag="OO327_002041"
     CDS             12397..13029
                     /locus_tag="OO327_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR6018587.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13411..16197)
                     /gene="polA"
                     /locus_tag="OO327_002042"
     CDS             complement(13411..16197)
                     /gene="polA"
                     /locus_tag="OO327_002042"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR6018588.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16561..17493
                     /locus_tag="OO327_002043"
     CDS             16561..17493
                     /locus_tag="OO327_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6018589.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17534..18964)
                     /locus_tag="OO327_002044"
     CDS             complement(17534..18964)
                     /locus_tag="OO327_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR6018590.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(19119..19745)
                     /gene="dsbA"
                     /locus_tag="OO327_002045"
     CDS             complement(19119..19745)
                     /gene="dsbA"
                     /locus_tag="OO327_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR6018591.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(19762..20748)
                     /gene="srkA"
                     /locus_tag="OO327_002046"
     CDS             complement(19762..20748)
                     /gene="srkA"
                     /locus_tag="OO327_002046"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR6018592.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(20825..21094)
                     /locus_tag="OO327_002047"
     CDS             complement(20825..21094)
                     /locus_tag="OO327_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR6018593.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            21164..21748
                     /gene="mobA"
                     /locus_tag="OO327_002048"
     CDS             21164..21748
                     /gene="mobA"
                     /locus_tag="OO327_002048"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR6018594.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            21730..22257
                     /gene="mobB"
                     /locus_tag="OO327_002049"
     CDS             21730..22257
                     /gene="mobB"
                     /locus_tag="OO327_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR6018595.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5515 a         5583 c         5334 g         5849 t
ORIGIN      
        1 tttgcttcgc cgataggtgc aaaaaatacg gctcgctgtg taaagactat cgcgccaaag
       61 cccagcgtac atgccatccc taaatagacc ggcatacttt cgtgcggcag cataatcaac
      121 agtacgagcg cagcggtact gatgataaag gtgtagcata gatatttact cggcgatttc
      181 aggatcttat ctgaaatcat gccaccaatc ggtccaccaa tcattttcag acagtattgg
      241 ttaatgatgc cgtaagcccc caccagcgca acgggcaata gatagatgtt tttcaggaat
      301 ggaatgaaga atgtcaggcc acagtaaacc gcgtaaacga agaagacgtt gaaagcgata
      361 agccagatgg ttttgtctct cagcactgag gtcatactgg tgttttgcga tgctccgtct
      421 tcttttttca cctcaacgga cggtgcctct tctttgtcat tcaggacaaa gaaaataata
      481 atgcctacgg caatcacaat aagggaatag aaccagatgc ctgctttaaa acctaataag
      541 ccactgccaa accaggtaaa tactgccaac gcagaaaatg ccaccacggt atcgacaatg
      601 ccacgccctg tttcgaagaa gccaaacaac cgaccttgtt gttcgctatt gcccaatcga
      661 cttaccgact tgagcaagac cggccagttc atcatgtcgc aagtaacgcc aaatagcgcc
      721 cagacaaaga ggatgcccca ataccccggc attgtcgtca aataaacacc cagtaatcct
      781 gtcgcaatga gtgagaatga catggtgtat ctgcgcggta gtttatcggc aaaataaata
      841 gacagaaaaa agcccactgt ggtgacaaat gagtttaccg acatagcatt accaatttga
      901 ccattggtca aatggaaata ttcctgcatc gggatataaa acgcatcttt cagcgacggt
      961 aatttataaa ttgtaccgcc acacagtgtt aatagactaa ataacgccca tttctttttc
     1021 gtgagcataa catcccctaa gcgattttta aaatcactgt tttaaaggca ataagatacc
     1081 gaccattcat atggaatggt tttattaact gcgtatgagt taaaacttat tagtggttca
     1141 gaagatggtt tgcctcttgt ttgatttgtt gtagtacgtt acgcacatga tttatctgat
     1201 taattgcatc ttctttttct ttacgtaacc gcgcatccag taaatgattt tctggtaggg
     1261 gtgtttcact catctggcga taagggatcc acggattatc atcttcgtct tcaaagcgga
     1321 acgccgacgc atagtaatca acctcttctt ccaggccata tgccgtcacc tgcggctcat
     1381 catcgccaag gcagataagg tgcgttaata acgctttgaa tggcatatca ccctgacctg
     1441 aaatacaggc tttatgaccc aggccaccct gttctttaac gatcaaggca tctttaatat
     1501 ggacctgggt aatatgcggt gccatcgttt ttaaggcgtc gatgggatgc tcatttgcat
     1561 tgatcatgtt agcaaaatca aacagtaagg aaagagactc catctcactt tctttgacca
     1621 gcgacaccag ctcatgactt tttaaatctt catgctgctc aatcgtaaaa gtcaggccgc
     1681 tgtcctgata cgtttcccgt acataggcaa tgtcgttagc gataatcgat aatacgtcgc
     1741 gcaaattacc ttcataacat ggataaaaac gtacggacgt tgccccagtt ttcaacgcga
     1801 tggcgacggc ttcgtcaata gatgccttat ctgaagcgct ggtttcaata tgaatatcaa
     1861 ggttcagacg gcgggcttta tccccaaagg cagagagttc tttatcgtcc atattaccaa
     1921 gagaaaaacg ctcgccatcc agaacatgga ttttgacgcc acgtagatta ttttcactgg
     1981 cgatatcaag taaatcagca ggcaacactc tttccagccg catatttaaa tgaaaggcat
     2041 aagcatgtaa ataaagagga agagtgtcga cacgttgtag aatctttctt gcgttattta
     2101 ttgtcaccat aaaacaccta cagattaatg atgaggtcta cggcctcaaa tcgccaatat
     2161 tcaatatcta tctggacaat attgccttgc tggtcggcgc gatgcttttc aattaagatc
     2221 gctggcatac ccgcagaacc gccaagatac ttgctgatat cgcccggcat tctgacgggt
     2281 ttgaaaatca atttttttac taccgtctct ttttggtagc acttttccca gactgcagaa
     2341 aatgagtggt tttcaagttg ttcaataaaa cccggagcaa cttcaggatt aatatatgtt
     2401 tcgtgataga aaactttatg tccttccagc gccccccagc cggtgatgcg atagagttca
     2461 gttgatggcg tcactgcaat cagtggcgcg agcgtctcgg gaatatcgct ggtacggttt
     2521 ttctcggtaa acccccaaga aggctctcgc ccttgctcaa ttgcggcccg ctgaaagctc
     2581 gccgacagct ccggactgta attaaatcgc ggctgggtca cgaaccaccc cttacgatcc
     2641 ttacggaaga ttttggattc tgcctcgagg ttcaacaacg cctggcgcag cgtcatacgt
     2701 ttaatgccca gcagttctcc cagctctctt tctgaaggga gttttccacc cggcgtggtg
     2761 atgccatctt tcaaccaccg atccagtttc tcttttgcat tttctacggt ggattgattc
     2821 tccgccattg tttaatcctt ttggtttaaa ccagatgaag catcttacgc ttacgccaaa
     2881 aaagaaagca attaacggtg tatgactttg cagataattg tgacaaagct ctctattgac
     2941 gtaatcacag acttataagg gagatactcg cattggtgtg cgctcacgac aggtaaaaaa
     3001 aaacctgcca tcgctggcag gttttttatg actaaaaaac gaaattaatc gtctttcggt
     3061 gcgcggttgg cgcggcgacg atcgttttcc gtcaggtgac gtttacgaat acggatagag
     3121 gtcggagtca cttctaccag ttcgtcatca tcgatgaact ccagagcttg ttccagagtc
     3181 atgcggatag gcggaaccag aacaacagct tcgtcagtac cggaagcacg catgttggtc
     3241 agtttcttac cggtcaggca gtttacagtc aggtcgttag agcggctatg aataccgata
     3301 atctgacctt cgtaaacttc tgcaccgtga ccgaggaaca gcttaccgcg atcctgcaga
     3361 ccgaacagcg cgaacgcgac agctttaccc tgaccgttag agatcagtac accgttctga
     3421 cgctgaccca cttcgcccgg acgtacgtcg tcgtagtggc tgaaggtgga gtacagcaga
     3481 ccagtaccgg aagtcatggt catgaactca gaacggaagc caatcagacc acggcttggg
     3541 atcacgtagt cgagacgtac gcggccttta ccgtctggat tcatgttttt caggtcgcct
     3601 ttacgttcgc ccagcgcctg catcacagaa ccctgatgct gttcttcaac gtccagcgtc
     3661 acgttttcat acggctcttg tttacgaccg tctatttcac ggaagattac tttcggacgg
     3721 gataccgcca gttcgaaacc ttcacgacgc atgttttcga taagaacaga gaggtgcagt
     3781 tcaccacgac cagaaacgcg gaacgcatcg gcgtcttcgg tttcttctac gcgcagcgca
     3841 acgttgtgta ccagttcttt gttcagacga tcgaggatct gacgagacgt tacgaactta
     3901 ccttctttac cgcagaacgg cgaggtgtta acgcagaaga acatagaaac ggtcggctca
     3961 tcaacggaga gtgccggcag cgcttcaacg ttttgcgtgt cgcaaacggt gtcagaaatg
     4021 ttcagttcgc ccaggcccgt gatcgccacg atatcgccag cttccgccag atcggtttcg
     4081 atacgttcca gaccgaggtg gcccagcact ttaccgactt tcgcgttacg ggttttgcct
     4141 tcgctatcga tgatagtaac ctgctggttc ggcttcactt taccacgctt gatgcggcca
     4201 atgccgataa cgccaacata gctgttgtaa tcgagctgag aaatctgcat ctggaacgga
     4261 ccgtcaaggt caacgtccgg cgcaggaacg tggtcaacaa tcgcctgata cagcggggtc
     4321 atgtcttccg ccatatcttc gtggtccaga cccgcgatac cgttcagcgc agaagcgtaa
     4381 acgatcggga agtccagctg ctcgtcggtc gcgtcgaggt taacgaacag atcgaatacc
     4441 tgatccacaa cccaatcagg acgcgcgcca gggcggtcaa ctttgttgat aacaacaatc
     4501 ggcttcaggc cgtaagcaaa cgcttttttg gttacgaagc gcgtttgcgg catcgggccg
     4561 tcaaatgcgt caaccaccag cagcactgag tctaccatgg acattacacg ttcaacttca
     4621 ccaccgaagt cggcgtgccc cggggtatca acgatgttga tacggtaatc attccatttg
     4681 atagcggtgt ttttcgcgag gatggtaatc ccacgctctt tctccaaatc gttggagtcc
     4741 atcacgcgct cttgggtttc ggcacgagag tcgaacgtac cggattgttg gagcagcttg
     4801 tctaccaggg tggttttacc atggtctacg tgcgcgatga tggcgatatt acgcaatttt
     4861 tcgatcacaa ctttgcctca ggcattagaa atagcgcgtt attgtacacg gattaatcgc
     4921 actacaaaac aggatcacaa acatcctccg caaacaagta ttgcagagtc cctttgtgat
     4981 cgctttcacg gagcataaaa agggttatcc aaaggtcatt gcaccaatat ggtgcttaat
     5041 gtttccattg aagcactata ttggtgcaac attcacatca tggtgcagcc cttttgcacg
     5101 atgttaagca tgataacgcc ttttaggggc aatttaaaag ttggcacaga tttcgcttta
     5161 tcttttttac ggcgacacgg ccaaaataat tgcagatttc gttaccacga cgacaatgac
     5221 caatccagga gagttaaagt atgtccgctg aacacgtact gacgatgctg aacgagcacg
     5281 aagtgaagtt tgttgatttg cgcttcaccg ataccaaagg taaagaacaa cacgtcacta
     5341 tccctgctca tcaggtgaat gctgaattct tcgaagaagg caaaatgttt gacggctcct
     5401 cgattggcgg ctggaaaggc attaacgaat ccgacatggt gctgatgcca gacgcatcca
     5461 ctgcagtgat tgacccgttc ttcgccgact ccaccctgat tatccgttgc gacatccttg
     5521 aacctggcac cctgcaaggc tatgaccgtg acccgcgctc tattgcgaag cgcgccgaag
     5581 attacctgcg ttccactggc attgccgaca ccgtactgtt cgggccagaa cctgaattct
     5641 tcctgttcga tgacatccgt ttcggatcat ctatctccgg ttcccacgtt gctatcgacg
     5701 atatcgaagg cgcatggaac tcctccaccc aatacgaagg tggtaacaaa ggtcaccgtc
     5761 cggcagtgaa aggcggttac ttcccggttc cgccggtaga ctctgctcag gatattcgtt
     5821 ctgaaatgtg tctggtgatg gaacagatgg gtctggtggt tgaagcccat caccacgaag
     5881 tagcgactgc tggtcagaac gaagtggcta cccgcttcaa taccatgacc aaaaaagctg
     5941 acgaaattca gatctacaaa tatgtcgtgc ataacgtagc gcaccgcttc ggtaaaaccg
     6001 cgacctttat gccaaaaccg atgttcggtg ataacggctc cggtatgcac tgccacatgt
     6061 ctctgtctaa aaacggcgtt aacctgttcg caggcgacaa atacgcaggt ctgtctgagc
     6121 aggcgctgta ctacattggc ggcgtaatca aacacgctaa agcgattaac gccctggcaa
     6181 acccgaccac caactcttat aagcgtctgg tcccgggcta cgaagcaccg gtaatgctgg
     6241 cttactctgc gcgtaaccgt tctgcgtcta tccgtattcc ggtggtttct tctccgaaag
     6301 cacgtcgtat cgaagtacgt ttcccagatc cggcagctaa cccgtacctg tgctttgctg
     6361 ccctgctgat ggccggtctt gatggtatca agaacaagat ccatccgggc gaagccatgg
     6421 acaaaaacct gtatgacctg ccgccagaag aagcgaaaga gatcccacag gttgcaggct
     6481 ctctggaaga agcactgaac gaactggatc tggaccgcga gttcctgaaa gccggtggcg
     6541 tgttcacgga tgaagcgatc gatgcgtaca tcgctctgcg tcgcgaagaa gatgaccgcg
     6601 tgcgtatgac tccgcatccg gtagagtttg agctgtacta cagcgtctaa tagttgaagt
     6661 tgtactaccc ggcgcacaac gccgggattt agttgccgtg gaaactttca gcccatctct
     6721 gcatgggctt ttttctccgt caattctctg atgcttcgcg ctttttatcc gtaaaaagct
     6781 ataatgcact aaaatggtgc aacctgttca ggagactgct ttatggcaac aggcacgcag
     6841 cccgatgctg ggcagatcct caactcgctg attaacagta ttttgttaat tgatgacaat
     6901 ctggcgatcc attacgccaa ccccgccgcg caacaactgc tcgcccaaag ctcccgcaaa
     6961 ttgtttggta cgccgttacc ggaactgttg agctacttct cattaaatat cgagctgatg
     7021 caagaaagtc tggaggcggg gcaaggcttt accgataacg aagtgacgct ggtcatcgat
     7081 ggtcgctcgc atatcctttc tgtgacggcc cagcgtatgc cggacggcat gatcctgctg
     7141 gagatggcac cgatggataa ccagcgccgc ttaagccagg aacaactaca acatgctcaa
     7201 caagtagctg cccgtgattt agtgcgcggc ctggcacatg agattaaaaa tccgcttggc
     7261 ggtttacgtg gcgcggcgca gttactcagc aaagcgttac ctgacccatc actactcgaa
     7321 tataccaaag tgattatcga acaggcggac cggctgcgaa atttggtcga ccgtctgttg
     7381 gggccgcagc tgcccggtac gcgcgttacc gaaagtattc acaaagtggc tgaacgcgtg
     7441 gtaacgctgg tgtcgatgga actgccggac aacgtgcggt tgattcgtga ttacgatccc
     7501 agcctgccgg aactggcgca cgacccggat caaattgaac aggtcttgct gaatattgtg
     7561 cgcaatgcgc tacaggcgct ggggccggaa ggcggtgaaa tcattctgcg tacccgcacc
     7621 gcgtttcaac tgaccttaca cggcgagcgc taccggctgg cggcgcggat tgatgtggaa
     7681 gataacgggc cgggcattcc gcctcatttg caggatacgc tgttttaccc gatggtcagc
     7741 ggccgcgaag gtggcaccgg gcttggctta tccatcgccc gtaatttgat tgatcagcat
     7801 tcaggcaaaa ttgaatttac cagttggcca ggtcataccg agttctcggt ttacctgcct
     7861 atcaggaaat aaaggtgacg tttatgcaac gagggatagt ctgggtagtc gatgacgata
     7921 gttccatccg ttgggtgctt gaacgtgcgc tcgctggagc gggtttaacc tgtacgacat
     7981 ttgagaacgg cgcggaagta ctggaggcgc tggcgagcaa aacgccggat gtgctgcttt
     8041 cagatatccg tatgccggga atggacgggc tggcgctgct caagcagatt aaacagcgcc
     8101 atccaatgct tccggtcatc attatgaccg cacattccga tctggatgct gccgtcagcg
     8161 cctatcaaca aggggcgttt gattatctgc ccaaaccgtt tgatatcgac gaagcagtgg
     8221 cgctggttga gcgcgctatc agtcattacc aggaacagca gcagccgcgt aatattcagc
     8281 ttaacggccc aacgaccgat atcatcggcg aagcgcaagc catgcaggac gtgttccgta
     8341 ttatcggtcg gctttcgcgt tcttctatta gcgtgctgat taacggcgaa tccggcaccg
     8401 gtaaagaact ggtcgctcat gccctgcatc gccacagtcc gcgcaccaaa gcgccgttta
     8461 tcgcgctgaa tatggcagct atcccaaaag atttgatcga atcagaactg tttggccacg
     8521 agaaaggcgc gtttactggc gcgaatacca ttcgtcaggg gcgttttgaa caggccgatg
     8581 gcggcacgtt atttctcgac gaaattggtg atatgccgct ggatgtgcag acgcgtttgc
     8641 tgcgcgtgct ggcagacggt cagttttatc gcgttggcgg ctatgcgccg gtgaaagtgg
     8701 atgtgcggat tatcgctgcc actcaccaga atcttgaaca gcgggtgcag gaaggtaagt
     8761 tccgtgagga tctgttccac cgcctgaacg ttatccgcgt tcatctgccg ccgctgcgcg
     8821 aacgtcggga agatattccc cgtctggcgc gccatttttt acaggttgcc gcgcgcgaac
     8881 tgggcgtaga agcgaagttg ctgcatccgg aaaccgaagc tgctctgacg cgtctggcgt
     8941 ggccaggcaa cgtgcgccag ctggaaaaca cctgccgctg gctaacggtg atggccgccg
     9001 ggcaggaagt gttgattcag gatttgcccg gcgaactgtt tgaatcaacg gttgcggaga
     9061 gtacttcgca aatgcaaccg gacagttggg cgacactttt agcgcagtgg gcagacagag
     9121 cgctgcgttc cggtcatcaa aatctgcttt ccgaagcgca gccagagctg gagcggacgt
     9181 tactgacgac cgcgttgcga catacgcagg ggcataaaca ggaagcggcg cggctactcg
     9241 gctggggccg caacactctg acgcgtaagt taaaagagct ggggatggag tgattcacgg
     9301 cttgtgtgta aagattgatt attgagcgca aattgctggt attttgcgct ttactgttcc
     9361 gataagttca gtatgatctt gcccggaaaa cggggagagt cattatgctg gaatcaataa
     9421 ttaatctggt atcgagtggc gcagttgaca gccacacacc gcaaactgcc gttgctgccg
     9481 tgctgtgtgc cgcgatgatt gggctgttta gctgaagctc gttgtttatg ccggatgcgg
     9541 agaaagtcgt aggcctgata agacgcgacc agcgtcgcat caggcatcat gctcagatgc
     9601 ctgatgcggc gtaaacgcct tatccggcct acaaaagcaa taatttcaac aggttttgtt
     9661 gcccagcgca tcaggcaatc ttgttctcag cacattgatg gcgctttcgt tttctacttt
     9721 gtaaacgaag cgccattcac tctttaaatc acccgtgaga actgctgcat ccgcgctttc
     9781 tggcgcagat aggtatcaaa gcacatgcaa atgttgcgga tcagcaagcg acctttcgcc
     9841 gtcacctgta ttcccttctc atccacatcc accagcccat cttttgctaa cggggcgagc
     9901 agcttgagat cttccgcaaa gtaatcagcg aagtgcaaat cccactgttt ctcaagaggg
     9961 gcgtaatcca gacggaagtt gcagatgagc gacttaatca catcgcggcg aatacagtca
    10021 tcacgcgtta gcgcaatacc acgccacagc gcattgcctt gttcatacac ttgctgatag
    10081 tactgcttca actctttctg gttctgcgcg tagcagtcgc caatcatgct gatggcggaa
    10141 acgcccatcc ccagcagatc ggtatcgccc tgagtggtgt agccctggaa gttacgatgc
    10201 agcacgcctt cacgctgggc caccgccagc tcgtcatcag gacgggcaaa gtgatccata
    10261 ccgataaact gatagcccga ttgcgtcagg aaggcgatgg tttcctgcag gatatcgagt
    10321 ttttgctgcg gactcggcag gtcagcatct ttgattttgc gctgagcagc aaaaatggtc
    10381 ggcagatgcg cgtagttaaa aacgctcaga cgatcggggt tcagctccgc cacacgtttc
    10441 agggtaaagg cgaaactctc cggcgtctgt ttcggcaggc cgtaaatcag gtcgatgttg
    10501 gtggaggtaa aaccaatctc acgcgcatgg ttaagcagtg caaagatgaa ctcttcatcc
    10561 tgctcgcggt taaccagacg ttgcacttct ttgttgaagt cctgcacgcc catgctcagg
    10621 cgattaaagc cttcggcgcg taaatgatcg agtacatcca gttcgatttc ccgcggatcg
    10681 acttcgatcg aaatctccgc atcggtattg aaatggaagt tttcgcgcag cagcttcatc
    10741 agacggctga tttgcgcttt attcagatac gtcggcgttc cgccgcccca gtgcaattgg
    10801 ctgacgtgac gcccggcgaa cagcggtgca cgatggacga tttcttgctc cagcgcgtcc
    10861 agatactgat cggccttgtg ctgctggcga gtaacaatct tattgcaacc gcagaagtaa
    10921 caaagcttat ggcagaacgg gatatgtacg tagagagata atggacgctc aggatagcgc
    10981 gccacggctt gtaaaaacgc ctgttcgccg aagtcttctg aaaactccag cgcggtcggg
    11041 tacgaggtgt atcgtggccc ggaatagtta tatttctgga tcagggccag atcccagtcg
    11101 atttgctgta cagacatgct cactccttcc gatggcgtct ctggcggcta cggcgtaccg
    11161 gcggatgccc gtttcgggtc atcagccggt tgcgcagcca cttttgtcgc cgcgacaacc
    11221 gctgtagttt aacgaataac cacaccagat aacatataac cggaagggtt atcagcagaa
    11281 cgggaaggcc cacggcggca atccgttagt tgccccgcag cagacgcatc atgtcttctt
    11341 gcttctcgtc ttcttcctct tcttcgtcat cgtcataaga gaggccgagt ttctgcatca
    11401 actcatcaat acgatccagt ttagcatcca cccaggattg ctcttcggcg ctcagggttt
    11461 cgcctgcttc cagacgttcc agcagcgcat ccagacactc atccgtttcc agtaattcca
    11521 actccgcctg cggtgaaagc ataggtttct cactcttcgg tttgtgctgt ttggtgactt
    11581 tttcagtcac tcccaatgga ataggggttt tactgccaat acgtggatct tttggtgcgt
    11641 tctggccttt gctgcctgac gtggtgtttc cgcccgctgc gcggctgccc ggcgcatgac
    11701 cacgacgttt tttctgacgc ttacggtcac gagcttcctg atccagctcc tcgcgtgttt
    11761 ttcgacgcgc ttttgcatga cctttgctgc gtgagtttga agatgatggt ttcataatgg
    11821 ttcgtctttt agccgtttta ttcagtatag atttgcggcg gaatctaaca gaaagcaagc
    11881 aaagaaaaaa ggcgacagat tactctgtcg ccttttttcc tgactcataa cccttaacgg
    11941 gtcttacaat ccttgcaggc cgccaacaca ccctgttttt ccattagccg gaacaatccg
    12001 tgttgattcc atttccgttt aattacgtct ccggacgttt gtcttcctga caatcctctg
    12061 tcttcgcctc ctggcgctcc tgaccctcat cctgagtcat tgttcctgtt agcgtcctcg
    12121 ctctatgggt gctactttac gcctttgctt taaacaaaca atcaaccgcc atcagtattt
    12181 gcacattttc agagttttat ataaaagcaa ttcactgatt tatatagctt atttgacatg
    12241 catcgcagaa ttttctctgc tatttcgcat aaagactatc ggcaatgtct cacaaagcgt
    12301 atggctttta cttacaacgc tcacgccgaa aaatccgcct tttattatgg ttcaggtata
    12361 atcgccacaa agctttgact gacggagacg accgctttga ctaatttgaa ttatcaacag
    12421 acgcattttg tgatgagtgc gcctgatatt cgccacctac cttccgatac cggaattgaa
    12481 gtggcttttg caggccgttc caacgcaggt aaatccagcg cgctgaacac gctgactaac
    12541 cagaaaagcc tggctcgtac ctcaaaaacc ccagggcgca cccagcttat caacctgttt
    12601 gaagtggctg acggcaagcg tctggttgac ttgcctgggt acggttatgc ggaagtcccg
    12661 gaagagatga agcgcaaatg gcagcgtgcg ctcggcgaat acctcgaaaa acgtcagagc
    12721 ctgcaaggtc tggtggtgct aatggatatt cgccatccgc tgaaagattt ggatcagcag
    12781 atgattgagt gggcggtaga cagcaatatc gccgttctgg tgctgctgac caaagcggac
    12841 aaactggcaa gcggcgcacg taaagcgcta ttgaatatgg tgcgtgaagc tgtactggcg
    12901 tttaacggtg atgtgcaggt tgaaacgttt tcttcgttga agaaacaagg cgtagacaag
    12961 ctgaggcaga aactggatac ctggtttagc gagatgcagc ctgtagaaga aacgcaggac
    13021 ggcgaataat tttcttgcct taatgcttgt gccggatgtg gcgtatccgg cccgtaaatt
    13081 caatagatta caattctacc gttggcctga taaacatagc gcatcaggca ttacggatct
    13141 tttctttcgc ccaataaaaa acgccccagt cattactgac tggggcggct aaaatattca
    13201 gccaaatccg attacgtgaa gtaaaaggtc tgaaagatag aacatcttac ctctgtaccc
    13261 tacgcgacta actttactct tttttgttca gttcagaaag cactttttgt aatttttttt
    13321 cagttgttgc ataggaattt ctaatagcca tcacaaaacg tgacagctta tgttgcttac
    13381 ttacgaaaaa agggcatgtt caggcgaatc ttagtgcgcc tgatcccagt tttcgccact
    13441 ccccacttcc accagcaacg gcacatccag acgggtacag ttttccatca gttgatgaat
    13501 ctgcttcgcg acggcatcaa catcatcttt atgaacttca aataccagtt catcgtgtac
    13561 ctgcatgatc atacgtacac gcggttgctc agcctgtaac cacgcatcaa cggcaatcat
    13621 cgcccgtttg ataatgtcgg cggcggttcc ctgcattggc gcgttaatgg ctgcacgttc
    13681 agccgctgca cgacgagcac cattgctgga tttgatatcc ggcagataca gacggcgtcc
    13741 gtccagcgtt tcaacgtagc ccagctcttt cgcctgagca cgggtgcgtt ccatatactc
    13801 cagcacgcca gggtagcgtt cgaagtaaag gtccatgtac ttctgcgctt ctttacgtgg
    13861 aatgttcaat tgccgcgcca gaccgaaagc actcatgcca taaatcagac caaagttgat
    13921 cgctttcgcg ctacggcgtt gctcgctggt gacggtttcc agtggcaaac caaacacttc
    13981 tgccgccgtt gcccggtgga tatcttttcc ttccgcgaat gcggtcagca agcctttatc
    14041 acgcgagaga tgcgccataa tgcgcagttc aatctgcgag tagtccgctg agacaatcac
    14101 ataatcctct ggcgcaataa acgcctggcg gatacgacga ccttcttcgt tacgcaccgg
    14161 aatgttttgc aggttaggat cggttgacga taaacgtccc gttgcagtta ctgcctggtg
    14221 ataagaggta tgcacacgcc cggttttcgg gttgatcatc agcggcagct tgtcggtgta
    14281 agtcgacttc agcttcgcca gaccacgata ctccagaatc acttttggca acggatagtc
    14341 cagcgccagt tcttccagta cctcttccga cgttgacggc gcgccacccg gcgttttctt
    14401 cagcggttta ataccctgtt tttcaaacag aatggtttgc aactgcttgg tggaagaaag
    14461 gttaaattct tcacctgcaa tttcatgcgc tttcttttcc agctcagcca gacgcaaggt
    14521 gagctcttca gaatgattgt gcagcacttt cggatcgatc ttcacaccgt tacgttcaat
    14581 gcgtgaaagc accggcacca gcggcatttc gatattctcg aagacgttca acggcccttt
    14641 gtgtttctgc agatccggcc acattttcag atgcaactgc aaggtgacat ctgcatcttc
    14701 ggcggcgtaa cgtccggcct cttcgagggc aatctggtta aaggtcagtt gatttttgcc
    14761 tttacccgca atctcttcaa aagtgatggt tttgtgcttc aaccaacgtt ccgcgaggct
    14821 gtccatatcg tgacgcccgg caacgctatt gagaatgtag gactccagca tggtatcaaa
    14881 cgcaatccca cgcagttcaa tgccgtaatt cgccagaata ccgcgatcgt atttcaggtt
    14941 ttgcccgacc ttcagcgcct tttcatcttc cagcagcggt tttagcaact cgagtgcacg
    15001 ctcgtgagag atttgatcgg gcgcatcaag ataatcatga gcaaccggaa tatatgccgc
    15061 tacgcctggc tcgatagcaa aagaaagccc gaccaggtta gcagagatgt tatcaaggct
    15121 gtcggtttcg gtatcaaatg caaataccgg cgctttttcc agcttcgcaa tccacgcttt
    15181 cagtgtttct tcatcaagga tagtgacgta gttgtcataa gaaatcaccg ttgccgtcac
    15241 ttctggcgct tcgtctgcaa cactggtttc ctgaggcttc gcggctgttt ttgccccttt
    15301 ggcctgtaac catttgccca cttcgacatc agcagtccag cgtttgaact catacttttt
    15361 gaacagcccc aacaactctt ccgctgccgg ttgctgcact tccagttgtt cacaggtcag
    15421 ctccagttca acatcggttt taatcgtcgc caactggtat gagagataag caacttcttt
    15481 gttttgctcg agcttcgctg ccattgtttt cgcgccacgg aagctcaacc cagcaatttt
    15541 ttctggctcg gcatacagcg tatccagccc gccaagacct tgcagcaatg cctgcgcggt
    15601 tttttcaccg acgcccggta cgccaggaat gttatcagag gagtcaccca tcagcgccag
    15661 gaagtcgatg atcaattctg gcggcacgcc gtacttattc accacctctt ccggtccgag
    15721 gatggtattc gtcatggtat tgataagcgt aatatttggc gtcaccagct gcgccatatc
    15781 tttatcgcca gtgctaatca gcaccggacg cccggctttt tcggcttcgc gcgccagagt
    15841 accgataacg tcgtccgctt ctacgccaga aaccgccagc agcggcagtc ccatcgcttt
    15901 aaccatcgcg tgcagaggtt cgatttgtgc tcgcagatcg tccggcattg gcgggcgatg
    15961 tgatttgtaa tgttcaaaca gttcatcacg aaaggttttt cccttggcgt caaagaccac
    16021 cgctgcatgc gtcggtttat attgcatgat cagactgcgc agcatgttga ggacaccata
    16081 catcgcaccg gtcggctcgc ctgcgctgtt agtcagcggg ggaaacgcgt gatatgcgcg
    16141 ataaagataa gatgaaccat ctacaaggat aagtggattt tgggggatct gaaccataat
    16201 gtccgtgcct gtttatcaga ttatgggtaa aggatgccac agacaggatg aaaacatgag
    16261 tttttcaccg cttttcgtga aaatcttttg tagatctgct ggatcgctcg caaatcgtca
    16321 tgtggataac tttgtgcata aattatttac atcgctttta atttattatc atgcttttta
    16381 cagcatgatg tttgtctttg tttttcatgc agttaaaaat aaatccatcc ctaataattg
    16441 agataatggg attttttcta atgtggatag atatgaatta tttttctcct taaggatcat
    16501 ccgttatttg ggtcattttc tgctaaaatc tgcgccttgc gcttctttag tgcactgtct
    16561 atggctaacc ttttgaataa attcattatg acgagaatac tcgctgcgat aacccttttg
    16621 ctgagtatcg tattgaccat tttggtcact attttttgtt ccgtcccgat catcattgcc
    16681 gggattgtaa aactattgct acctgtgcca gtcatttggc gaaaggtttc gcgtttttgt
    16741 gattttatga tgtattgctg gtgtgaaggt ctggcggtat tactgcatct gaacccacac
    16801 ctgcaatggg aagttcacgg gctggaaggg ttaagtaaga agaactggta tctgcttatt
    16861 tgtaaccacc gtagttgggc agatattgtc gtactgtgcg tgctgtttcg taagcacatt
    16921 ccgatgaata aatatttcct caagcaacag ctggcctggg tgccatttct tggcctggcg
    16981 tgctgggcgc tggatatgcc gtttatgaag cgttattccc gcgcttattt attacgtcat
    17041 ccggagcgac gtggtcaaga tgttgaaacc actcggcgtt cttgtgaaaa gtttcgcctg
    17101 catcccacca ccattgtcaa tttcgttgaa ggctcccgtt tcacgcaaga aaaacatcag
    17161 caaacccact ccacttttca aaacttgttg ccaccaaagg cggcaggcat tgcgatggcg
    17221 ctaaatgtac tgggtaaaca attcgataaa ctgcttaatg ttacgctgtg ttatccggac
    17281 aataaccgtc agccgttctt cgatatgtta agcggtaaac tgacgcggat tgtcgtacat
    17341 gtggatttac agcctattgc cgatgagtta catggcgact acatcaatga caaaagcttt
    17401 aagcgccatt ttcagcaatg gctgaactca ttgtggcagg aaaaagaccg gcttctgacg
    17461 tctctcatgt catcacagcg tcagaataag taagttttac tcgcctctga atgagcagag
    17521 gcgagtgagt attttaatga acaaaacgtc cggcacgaga cataaattct tcttcgctca
    17581 tctcctgtcc gttaaaaaca actttacccg gtgtataacg cagctgcagc gaggcggtat
    17641 tgttgtcaat cgtgattaac tgaaacatct gacctaatgt catcatcccg ctgatttgtt
    17701 tatcagcctg cttttgcgct ttttccgcat ccattccttc agataaatta agctgttttg
    17761 cggtttctgt gacgacatta agcggtaact tcacatcaaa attgagcgat ttgatatctt
    17821 tgtttgtgga tgatgaagat ttggctgggt cggcaatact gatatccaga ttggcattta
    17881 gttcgccgag tgcgttcttc catcttaccg gttgtttaat ggtcggctca cttttttgta
    17941 ataacggtaa atactctttg aacaatgcag cattcacttc ttgcagagca acttcatcgt
    18001 ttgctaactc agggtgtgca gcaagctgct tctgcatcgc aatgttatac tggatgataa
    18061 attggcgcac tgctgaggga tcaatagatt cagcaatcaa tgaccactca ccactaccga
    18121 gttgctgatt cccttttgtt aacttatcaa gttcgtaggt cagattgaca ttgatataat
    18181 ctttgtcttt aacgcgatcc agcctggttt ttgcaacgaa agcatcgatt tgggcaacgt
    18241 cttccccgtg attgataatg ttaatctgat taaatttcga ctcgctttca ccaaccggaa
    18301 atttcttttc ttctaatcgc gccagagaat caatggtaaa actttttgcc gttactttgt
    18361 tcattggtga taattgcaga acaatatctt cgactttgcc ttcaatatcc aggttagaca
    18421 atgaactacc atcaccatta aaagtaaact ggccctcacc aaaagaaaat tttccatact
    18481 ctgcaggggc aacatctaat gtcgtagaga atgagccacc aaacgcgaat cgcaatgtcg
    18541 cttccacggg cgatttattt ttcgcggcga taaatagcgg ttgtgtcagt tcattattca
    18601 ctaagttcac ttttgccgct gccagtgctg ggatgacatt accatgcatt aacattgtga
    18661 tgggcaacgg accgtgctca acatccacat caaatacaac tttctggcct ggcttgatat
    18721 tgagatcggg tgcaccgtta tcgaatgtca tttgcatttg aaaacgggaa ttaaaaaagc
    18781 ctttgtcaaa gtttttataa cttaacgtca tatcgtaggc atgttcacct ttctttttcg
    18841 catcgttaaa atctttaata aacttttcga taccaggctg aatttgcgta cctgtgtacc
    18901 atgtgccacc accccagatt acggctaacg caacaataac acctgtagcg gacttacgta
    18961 tcatataaat attccctgat aaaacagcaa aactttgaat atccacttat gctgaaataa
    19021 tgtccagcgg caggatgcat tatcagaatg tatttatatt tattaaataa taaaaaaagc
    19081 ccgtgaatat tcacgggctt tatgtaattt acattgaatt atttttcctc ggacagatat
    19141 ttcaccgtat cagcatactg ctgaacaaaa acatccatat tgctggtatc cataccctgc
    19201 ggattcagct gatatttacc gttaacaaac atcgccggaa caccacgcaa ttgcacatca
    19261 gctgcggctt tttcctgctg agcgaccaga gatttcacca cgaagctgtt ccacgccgcg
    19321 tcgtactctt cacctttaat acctgcattg ataaatacat cgcggatatc agatgctgaa
    19381 cgaatggtct gggttttctg tacgccttca aacagcggaa cagtcacttt gtcttccacg
    19441 cccagcgcca tcgccacagc ccatgcctga gtcagatctt tgcccaggtc accacccatg
    19501 aagttgacgt ggtatttagt catcttcacg ccttccggca gttttttctt cacattatca
    19561 gaaatatgca gaacttcttc aaactgatag cagtgcgggc agaagaaaga gaaaaactcc
    19621 agcacttgcg gcgcgccagc taccggtttt tccagggtag tgtactgttt accatcttca
    19681 tactgcgccg ccgatgcgct aaacgctaaa actaaaccag ccagcgccag ccaaatcttt
    19741 ttcatgatct actctctccg attaatacat aggtgttaat tgcaaagggg gttcttgtag
    19801 aacttttgcc tgttctataa aagtcgccgt ctgtcgtagc cagtaatctt ccccggttaa
    19861 ccacgggaaa tttttcggga acgcgggatc agcccaatgc cgcattagcc aggcaagata
    19921 ataaaccaaa cgcatggcgc gtaaaggttc aatcagtccg atttcagcgg tgtcgaactc
    19981 gctaaattct tcataagctt caataatagt ttccagttgc atccgctgct gggctttatc
    20041 gccattgagc aacatccaca aatcctgaat agctggacca ttacgtgcat catccagatc
    20101 aacaaacatt ggaccatcgc gccagagaat attcccggcg tggcagtctc catgtagccg
    20161 cagaacggtg aaatcttccc gccagtgtgc tgtaacggcg gcgatcagct catctgtcgc
    20221 tttcagaaat gccgctttca acccggaagg tatcagtgta gcgtcctcaa acagcttgcg
    20281 aggctcaatg agatattcat tcagaccgat ggtcgggcga tgaataaaaa gctgtttgcg
    20341 ccccgtctgg tgcatacgcc ctaaataacg cccaaccgct tccatctgat cgatattatc
    20401 agcttcgaac tggcgaccac cgacgcttgg aaaaacagcg aaataaaatc cctgatgatt
    20461 caataaagtc tgaccgttaa aggccacagg tgctgcgacc ggaacttcat catttaccag
    20521 ctgcaacgca aattgatgtt cttcgaggat ttgatcggct gtccaacgtt cagggcgata
    20581 aaatttgacg acaaaacgtc gacgctcttc gtcctgaaat tgatagacac ggttttcata
    20641 gctgttaagc ggggtaagac cggaatccac ccggatccct tgcttaaaca gagcgtccat
    20701 gatggtatcc gggtgtagtg tctggaaagt aaaagcgctg ttgttcatcc catcatccgg
    20761 aaaacattac gaatgatgaa ggatatcatt tcacgatgat tttggtggcg ccgcttacaa
    20821 gcttttactc tttaattacg ccgcgagcgc gtaacagcgc cgttttgaag tcttcttcat
    20881 aatctttctg caaacccggg atcaccgcat ctttcgcgga atcacgcatt ttcaggtgat
    20941 agatcagaat gtcatccgtc aaatccgcca gttcgccgtc aaaacctgac tctttcgcca
    21001 gtttctgcaa aaattgcaac aagttaaagt ctggctcttt ttgccaggct ggctggagga
    21061 gttcaataac ttcattcaga cgtttacatt tcatagtaat gctccttact cttgagacag
    21121 acacgttagc agggtcaatc ccacaataaa agaggcgata tcggtgaatc taatgacgac
    21181 gataacaggc gttgtgctgg caggcggtaa agccagacga atgggcggcg tagataaagg
    21241 attgctggac ttaaatggca aaccgctgtg gcaacatgtc gctgacgcgc ttaagacgca
    21301 gctctctcac gtcgtggtta atgctaatcg tcatcaggaa atctatcagg caggcggtct
    21361 gaaagtgatt gaagattcac tggcggatta cccaggccct ctggcaggaa tgctttcagt
    21421 aatgcagcag gaagctggtg agtggttttt gttttgtccg tgcgatacgc cttacattcc
    21481 ccatgattta gccgcccggc ttaatcatca gcgcaaagat gcgcctgtcg tgtgggtcca
    21541 tgacggtgaa cgcgatcacc cgactattgc tctggtaaac cgcgctattg agcctttatt
    21601 actggaatat ctgcaagcag gagaacgccg ggtaatggca tttatgcgtc tggctggcgg
    21661 tcatgcggtt gatttcagcg atcataaaga tgcatttgtt aacgtgaata cgccagagga
    21721 gcttgcccga tggcaggaaa aacgatgata ccgttactcg cctttgccgc gtggagtggt
    21781 accggaaaaa ctacgctttt gaaaaaactg atcccggcat tatgcgccag agggatccgt
    21841 ccggggctga ttaagcatac gcaccatgat atggatgttg ataagccagg caaagatagc
    21901 tatgagctgc gcaaggctgg cgcggtacaa accatcgttg ccagccagca acgatgggcc
    21961 ttgatgacgg aaacaccaga cgaagaagag ctggatctac attttctcgc aagtcgaatg
    22021 gatacctcaa agctggattt gattctggtc gaagggttta agcatgaaga gatcgcaaag
    22081 attgtgctgt ttcgcgatgg agccggacat cgacctgaag aattagtgat agacaggcat
    22141 gttattgctg tagccagtga tgtgccgctt aatcttgatg tcgcattact ggatattaat
    22201 gatgttgagg ggctggctga ttttgtggtg gagtggatgc aaaagcagaa cggataagca
    22261 agagtaggct ggatcagacg c
//
LOCUS       DAJTSI010000060        21731 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000060 DAJTSI010000000
VERSION     DAJTSI010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21731
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.060"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..465
                     /gene="tnpA"
                     /locus_tag="OO327_002050"
     CDS             <1..465
                     /gene="tnpA"
                     /locus_tag="OO327_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR6018596.1"
                     /translation="RRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEKLRTYFSNVCADF
                     AAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRPDIAVRYYYKGVL
                     WSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFTAHRIKD"
     gene            complement(504..851)
                     /gene="grxD"
                     /locus_tag="OO327_002051"
     CDS             complement(504..851)
                     /gene="grxD"
                     /locus_tag="OO327_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR6018597.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            1186..2013
                     /gene="mepH"
                     /locus_tag="OO327_002052"
     CDS             1186..2013
                     /gene="mepH"
                     /locus_tag="OO327_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR6018598.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            2141..2722
                     /gene="sodB"
                     /locus_tag="OO327_002053"
     CDS             2141..2722
                     /gene="sodB"
                     /locus_tag="OO327_002053"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR6018599.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(2868..4037)
                     /locus_tag="OO327_002054"
     CDS             complement(2868..4037)
                     /locus_tag="OO327_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6018600.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(4203..4292)
                     /gene="ynhF"
                     /locus_tag="OO327_002055"
     CDS             complement(4203..4292)
                     /gene="ynhF"
                     /locus_tag="OO327_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR6018601.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
     gene            4591..5616
                     /gene="purR"
                     /locus_tag="OO327_002056"
     CDS             4591..5616
                     /gene="purR"
                     /locus_tag="OO327_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR6018602.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(5613..6545)
                     /locus_tag="OO327_002057"
     CDS             complement(5613..6545)
                     /locus_tag="OO327_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6018603.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            6658..7869
                     /locus_tag="OO327_002058"
     CDS             6658..7869
                     /locus_tag="OO327_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR6018604.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            8160..9308
                     /gene="cfa"
                     /locus_tag="OO327_002059"
     CDS             8160..9308
                     /gene="cfa"
                     /locus_tag="OO327_002059"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR6018605.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(9348..9989)
                     /gene="ribE"
                     /locus_tag="OO327_002060"
     CDS             complement(9348..9989)
                     /gene="ribE"
                     /locus_tag="OO327_002060"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR6018606.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            10204..11577
                     /gene="mdtK"
                     /locus_tag="OO327_002061"
     CDS             10204..11577
                     /gene="mdtK"
                     /locus_tag="OO327_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR6018607.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(11618..12874)
                     /locus_tag="OO327_002062"
     CDS             complement(11618..12874)
                     /locus_tag="OO327_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR6018608.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            13182..13258
                     /locus_tag="OO327_002063"
     tRNA            13182..13258
                     /locus_tag="OO327_002063"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13216..13218,aa:Val,seq:gac)
     gene            13263..13339
                     /locus_tag="OO327_002064"
     tRNA            13263..13339
                     /locus_tag="OO327_002064"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13297..13299,aa:Val,seq:gac)
     gene            13447..13752
                     /locus_tag="OO327_002065"
     CDS             13447..13752
                     /locus_tag="OO327_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR6018609.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            13878..15300
                     /locus_tag="OO327_002066"
                     /pseudo
     CDS             13878..15300
                     /locus_tag="OO327_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(15312..16124)
                     /locus_tag="OO327_002067"
     CDS             complement(15312..16124)
                     /locus_tag="OO327_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018610.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(16128..16913)
                     /locus_tag="OO327_002068"
     CDS             complement(16128..16913)
                     /locus_tag="OO327_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCR6018611.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(16910..17578)
                     /locus_tag="OO327_002069"
     CDS             complement(16910..17578)
                     /locus_tag="OO327_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR6018612.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(17642..18280)
                     /locus_tag="OO327_002070"
     CDS             complement(17642..18280)
                     /locus_tag="OO327_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCR6018613.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(18293..20394)
                     /locus_tag="OO327_002071"
                     /pseudo
     CDS             complement(18293..20394)
                     /locus_tag="OO327_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(20415..21041)
                     /locus_tag="OO327_002072"
     CDS             complement(20415..21041)
                     /locus_tag="OO327_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCR6018614.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGVLKIIEWKDITV"
     gene            complement(21497..21706)
                     /gene="fumD"
                     /locus_tag="OO327_002073"
     CDS             complement(21497..21706)
                     /gene="fumD"
                     /locus_tag="OO327_002073"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCR6018615.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         5431 a         5632 c         5046 g         5622 t
ORIGIN      
        1 cggcgtggaa ggcattgtgt tttcctgatg catgttcacc tggtctttgt caccaaatac
       61 cgacgccaga tttttgacca tgacacgaca gaaaaactac gcacttactt ttcaaatgta
      121 tgtgctgatt ttgcagctga actggttgaa atggatggcg aaccagatca cgtccatttg
      181 ttaattaact atcctcccaa actggcgata tccagtctgg taaacagcct caaaggcata
      241 tcgaataggt tactgcgacg agatcgacca gatattgcag tcaggtatta ctacaaaggc
      301 gttttgtgga gtcctggcta ttttgccagt agctgcgaag gtgcgccaat atccgtcatc
      361 cgccagtaca ttgaacaaca gcaaacacct ggtcaggtgg aaaaccgcgc cttatatccc
      421 cgccctgaag gacgaggttt tacggcgcac cggataaagg actgacggat gcccgttcgc
      481 atccgtcagt attgcaggac ggattattcc gcgtccggct cttcagactt gtatttagcg
      541 gcagtttctt tgatcagctg ctgcagttcg ccacgctgat acatttcgat cacgatatca
      601 caaccgccga ccagctcgcc gtcaacccac agttgcggga aggtcggcca gttagcatat
      661 ttcggcagtt ccgcacgaat gtccggattc tgcagaatat caacataggc aaaacgttcg
      721 ccacatgcgg caagcgcctg gactgcctgg gcagagaaac cgcagctcgg cagtttcggt
      781 gaacctttca tgtacagcag gatcgggttt tcagcaatct ggcgttggat tttttcgata
      841 gtggtgctca ttgtcttgct tcctcaaact tctttacggc agtaatctga cattgtagcg
      901 ggtcagtgcg gcatcggaaa ataacatttt catcacgctt ttgctatttt atccctttgc
      961 tctatttgtt gcatttcaaa tattcgtttc ttctctgatg cgcggcaaac tggcgtattt
     1021 ataaccattt ttagctttca ctgctgcatt tttttgcgct cgccaacgaa acgtattttt
     1081 taacaataaa agctattaac tttctcttct tctatgcatt agaatcatca agttttgtaa
     1141 atcagacgca ggcatgatag acctgcctta acagagggac gctcagtggc gcggataaac
     1201 cgtatttcga tcacgctctg tgctttactt tttaccaccc tgcctttaac gcctatggcc
     1261 catgcttcaa agcaagccag ggagagttct gctaccactc atatcaccaa aaaagcagat
     1321 aaaaagaaaa gcacggcaac caccaaaaag acccagaaag ccagccagaa aacagcgaaa
     1381 aaagccgcca gtaaaagtat gaccaaaagc aaaaccgctt cttctgttaa aaaatcttcc
     1441 attaccgctt ctaaaaacgc caaaactcgc agcaaacacg ccgtcaataa aacggcctca
     1501 gccagcttta ccgaaaagtg taccaaacgt aagggctata aatcgcattg tgtgaaagtc
     1561 aaaaatgccg cgtcaggaac ccttgccgac gcgcacaaag cgaaggtgca aaaagccacg
     1621 aaagtggcaa tgaataaact gatgcagcaa attggtaagc catatcgttg gggcggcagc
     1681 tcaccgcgta ccggttttga ttgcagcggc ctggtttatt acgcttataa agatttggtg
     1741 aaaattcgta ttccgcgtac ggcgaatgaa atgtatcacc tgcgtgatgc aggacctatc
     1801 gaacgtagtg agctgaaaaa cggcgacctg gtctttttcc gtactcaggg acgcggtaca
     1861 gccgatcatg tcggcgtgta tgtcggcaac ggcaaattta ttcagtcacc gcgcacaggt
     1921 caggaaattc aaatcacttc tctcagtgaa gactactggc agcgccacta tgttggcgct
     1981 cgtcgggtaa tgaccccaaa aacacttcgc taaaacttta ccctgttgtt acggcaacag
     2041 ggtaagttca tcttttgtct caccttttaa tttgctaccc tatccatacg cacaataagg
     2101 ctattgtacg tatgcaaatt aataataaag gagagtagca atgtcattcg aattacctgc
     2161 actaccatat gctaaagatg ctctggcacc gcacatttct gcggaaacta tcgagtatca
     2221 ctacggcaag catcatcaga cttatgtcac taacctgaac aacctgatta aaggtaccgc
     2281 gtttgaaggt aaatcactgg aagagattat tcgcagctct gaaggtggcg tattcaacaa
     2341 cgcagctcag gtctggaacc atactttcta ctggaactgc ctggcaccga acgccggtgg
     2401 cgaaccgact ggaaaagtcg ctgaagctat cgccgcatct tttggcagct ttgccgattt
     2461 caaagcgcag tttactgatg cagcgatcaa aaactttggt tctggctgga cctggctggt
     2521 gaaaaacagc gatggcaaac tggctatcgt ttcaacctct aacgcgggta ctccgctgac
     2581 caccgatgcg actccgctgc tgaccgttga tgtctgggaa cacgcttatt acatcgacta
     2641 tcgcaatgca cgtcctggct atctggagca cttctgggcg ctggtgaact gggaattcgt
     2701 agcgaaaaat ctcgctgcat aataactgat ggcaaatgca gcattgcctg atgcgcttcg
     2761 cttaccatgc caacgcggat gaggcgttaa cagcgcattc ggcaagataa atcgcacgtt
     2821 gtcagcaact gtaacgcaga aggttatcct tctgcgtttt tgtttaatta gctgttagca
     2881 acgcaaactg tttcaggttg ttttctggct gacataaaca ccagcaataa tgccagtccc
     2941 gcgacaatcg cccccgtcac cggcacaaag ctgtatccca gcccagcgga aattaccgca
     3001 ccaccagcag ctgctcccag cgcatttcca agattaaagg caccaatatt gactgatgaa
     3061 gacagacctg gcgcttcact ggcgacatgc atcacgcgca tctgtaacgg aggtacaacc
     3121 gcaaaggttg ctgcgcccca caccaccatg ctaatagctg cgccgaactc attgcgggcc
     3181 aggaacggga ttgccagcat aatcaccatc agcagcaaca aaaagccttt caacgtgccg
     3241 ttaactgaac gatctgccag tttgccgccg agatagttac cgatagagaa tccgacacca
     3301 atcagcacca gcattgccgt gacgaacacc ggtgttgcgt gggtaatact ttgcagtacc
     3361 ggagagatat aggtgtagag ggtaaacatt gcaccagctc ccagtaccgt cgtcagcaat
     3421 gcagacagca cctgcggacg cattaatacc gccagctctt ttttcacttc aggtcgtgcc
     3481 cctgtaccac ctttaggtaa tgagaagaac agacttacca ttgaaatcac tcccagcccc
     3541 gccgttgcca gaaatgacat ccgccagccg atggtttcac ccaaccaggt cgccgccggc
     3601 acgccaccga tatttgccag ggttaacccc ataaacatag tggcaactgc gctggcctgt
     3661 ttatgttttg gcaccacgct tgcggccacg actgaaccca aaccaaaaaa tgctccgtga
     3721 ttcaggctgg tcaaaatacg tgaaagcatc agggtcatat aatccggcgc gatggcggaa
     3781 agaacgttgc ctagcgtgaa aattgccatc aggaaaatca acgcactgcg gcgggcacga
     3841 tgagaaagta gaagcgtcat cagcggcgcg cccaccatta cgccaactgc ataggcactg
     3901 attaacattc cggcagcggg aatcgagaca tccacaccgc gcgcaatgac gggcaacaag
     3961 cccattggcg agaactccgt tgtcccgata ccaaacgcgc caatcgccag cgccagcaac
     4021 ggatagttaa ttttcatgcc ttatctccac ctcttcgcgt cattacgcga tattcattaa
     4081 agtggcgaaa gcatgacagc aatcacaaaa aaatgaaaat aacaaaaaga gaaaacactt
     4141 ttgccatttt gctaacaaac aggaaggaga tgcgagggag aacgcgctcc ctcgagagga
     4201 aatcagtgca gcgcggcagt caaacccacg gctacgatca aaccgaggac gataaccgtt
     4261 gttaccagtg aaaatttaag gtcggtgctc atcaagtttt ctcctttttt attaccacac
     4321 aaaaagtgat attacgcatt tttacacact gtgatgaaaa aatctcccgt catttataat
     4381 gataagtgtt tttaccactt ccccttttcg tcaagatcgg ccaaaattcc acgcttacac
     4441 tatttgcgta ctggccattg accccttcct gacgctccgt gtcgtttttc cggcgtaccg
     4501 caacactttt gttgtgcgta aggtgtgtaa aggcaaacgt ttaccttgcg attttgcagg
     4561 agctgaagtt agggtctgga gtgaaatgga atggcaacaa taaaagatgt agcgaaacga
     4621 gcaaacgttt ccactacaac tgtgtcacac gtgatcaaca aaacacgttt cgtcgctgaa
     4681 gaaacgcgca acgccgtgtg ggcagcgatt aaagaattac actactcccc tagcgcggtg
     4741 gcgcgtagcc tgaaggttaa ccacaccaag tctatcggtt tgctggcgac cagcagcgaa
     4801 gcggcctatt ttgccgagat cattgaagca gttgaaaaaa attgcttcca gaaaggttac
     4861 accctgattc tgggtaatgc gtggaacaat cttgagaaac agcgggctta tctgtcgatg
     4921 atggcgcaaa aacgcgtcga tggtctgctg gtgatgtgtt ctgagtaccc agagccgttg
     4981 ctggcgatgc tggaagagta tcgccatatc ccaatggtgg tgatggactg gggtgaagca
     5041 aaagctgact tcaccgatgc ggtcattgat aacgcgttcg aaggcggcta catggccggg
     5101 cgttatctga ttgaacgcgg tcaccgcgaa atcggcgtca tccccggccc gctggaacgt
     5161 aacaccggcg caggccgcct tgccggtttt atgaaggcga tggaagaagc gatgatcaag
     5221 gtgccggaaa gctggattgt gcagggtgac tttgaacctg aatccggtta tcgcgccatg
     5281 cagcaaatcc tgtcgcagcc gcatcgccct actgccgtct tctgtggtgg cgatatcatg
     5341 gcaatgggcg cactttgtgc tgctgatgaa atgggcctgc gcgtcccgca ggatgtttcg
     5401 ctgatcggtt atgataacgt gcgcaacgcg cgctatttta cgccggcgct gaccacgatc
     5461 catcagccaa aagattcgct gggtgaaaca gcgttcaaca tgctgttgga tcgtatcgtc
     5521 aacaaacgtg aagaaccgca gtccattgaa gtgcatccgc gtttgattga acgccgctcc
     5581 gtggctgacg gcccgttccg cgactatcgt cgttaatcac ccgttgcggg agtctcttcc
     5641 ggctcccgca gccactcctt attcagcgtc tcactatcgc tgagatactc aagcagccag
     5701 gttaacgcag gcgacatatc attttgctgc catgtcagac aacatgccga atccgggaag
     5761 gggttttcca gttcaagtgc tacccacttc ccctcattaa gccacggttt ggcgaaatgt
     5821 gttggcacca tccctatgca taatcctgcc gagatacagg ttgccgatga ttcccagtca
     5881 ggtacgacaa gtcttttttg gttatccagc aaccaggtaa tacgtttagg tagcgttcgc
     5941 gaggtgtctt cgcgcaccaa cgacggccag ttgcgcaacg tatcatcgct gaacgggcca
     6001 tccatcaacg ccagcgggtg gtgactggca acaacgcaac tccagcttag catccccata
     6061 tcccggaagg cataacgacc gcctaccgga atcgcgcgtg ttgcgccaat tgccagttcc
     6121 acgcgcccgt cggaaagcgc atcccagaca ccgttgaaca cttcctgaaa gacaagaagt
     6181 tcgacatcat caaaatggcg ataaaaatca acgatcatct gccgtgtacg ttctggcctg
     6241 acaatattat ccactgcgat agctaactga ccgcgccagc cgttcgctat ctgctgacat
     6301 tgctggcggg tgatctgcat ttttttgaca acagagcgcc cttctttgag aaaccacgct
     6361 ccagcagcgg tcagctccac atcacggtgc cgtcgttcaa agagcggtac cgccagccac
     6421 tcttccagct gacgcacggt atagctgacc gcagaaggaa cacgatgcag ctcctgtgcc
     6481 gcagcgctaa aactaccatt acgcgctacc gcatcaacaa cttcgagtga atattctgac
     6541 cacatagtct gcctgcaaaa tttttgaaac cagtcatcaa atattaccgt ttcacaacac
     6601 taatttcact ccctacactt tgcggcggtg tttaattgag agatttagag aatatacatg
     6661 caacctggga aaagattttt agtctggctg gcgggtttga gcgtactcgg ttttctggca
     6721 accgatatgt atctgcctgc tttcgccgcc atacaggccg acctgcaaac gcctgcgtct
     6781 gctgtcagtg ccagccttag tctgttcctt gccggttttg ccgcagccca gcttctgtgg
     6841 gggccactct ccgaccgtta tggtcgtaaa ccggtgttat taatcggcct gacaattttt
     6901 gcgttaggta gtctggggat gctgtgggta gaaaacgccg ccacgctgct ggtattgcgt
     6961 tttgtacagg ctgtgggggt ctgcgccgcg gcggttatct ggcaagcatt agtgacagat
     7021 tattatcctt cacagaaagt caaccgtatt tttgcgacca tcatgccgct ggtgggtctg
     7081 tctccggcac tggctcccct gttaggaagc tggctgctgg tccatttttc ctggcaggcg
     7141 attttcgcca ccctgtttgc cattaccgtg gtgctgattc tgcctatttt ctggctcaaa
     7201 cccacgacga aggcccgtaa caatagtcag gatggtctga cctttaccga cctgctacgt
     7261 tctaaaacct atcgcggcaa cgtgctgata tacgcagcct gttcagccag tttttttgca
     7321 tggctgaccg gttcaccgtt catccttagt gaaatgggct acagcccggc agttattggt
     7381 ttaagttatg tcccgcaaac tatcgcgttt ctgattggtg gttatggctg tcgcgccgca
     7441 ctgcagaaat ggcaaggcaa gcagttatta ccgtggttgc tggtgctgtt tgctgtcagc
     7501 gtcattgcga cctgggctgc gggcttcatt agccatgtgt cgctggtcga aatcctgatc
     7561 ccattctgtg tgatggcgat tgccaatggc gcgatctacc ctattgttgt cgcccaggcg
     7621 ctgcgtccct tcccacatgc aaccggtcgc gccgcagcgt tgcagaacac tctgcaactg
     7681 ggtctgtgct tcctcgcaag tctggtagtt tcctggctta ttagtatcag cacgccattg
     7741 ctcaccacca ccagcgtgat gttatcaaca gtagtgctgg tcgcgctggg ttacatgatg
     7801 caacgttgtg aagaagttga ctgcccgaat catggcaatg ccgaagtcgc tcatagcgaa
     7861 tcacactgat ctatatcgat atacttatac ttaggctgct aacaaaattt tgttgtatga
     7921 ttgaaattag cggcctatac taatttcgag ttgttaaagc tacgataaat attatgtttt
     7981 tacggggaca ggatcgttcc cgactcacta tggatggtca tttcggcaag ggttcctcct
     8041 ttccctctgt tctacgtcgg attatagact cgcggttttt tctgcgagat ttctcacaaa
     8101 gcccaaaaag cgtctacgct gttttaaggt tctgatcacc gaccagtgat ggagaaacta
     8161 tgagttcatc gtgtatagaa gaagtcagtg taccggatga caactggtac cgtatcgcca
     8221 acgaattact tagccgtgcc ggtatagcca ttaacggttc tgccccggcg gatattcgtg
     8281 tgaaaaaccc cgattttttt aaacgcgttc tgcaggaagg ctctttgggg ttaggcgaaa
     8341 gttatatgga tggctggtgg gaatgtgacc gactggatat gttttttagc aaagtcttac
     8401 gcgcaggtct cgagaaccaa ctcccccatc atttcaaaga cacgctgcgt attgccggcg
     8461 ctcgtctctt caatctgcag agtaaaaaac gtgcctggat agtcggcaag gagcattacg
     8521 atttgggtaa tgacttgttc agccgcatgc ttgatccctt catgcaatat tcctgcgctt
     8581 actggaaaga tgccgataat ctggaatctg cccagcaggc gaagctcaaa atgatttgtg
     8641 aaaaattgca gttaaaacca gggatgcgcg tactggatat tggctgcggc tggggcggac
     8701 tagcgcacta catggcatct aactatgacg taagcgtggt cggcgtcacc atttctgccg
     8761 aacagcaaaa aatggctcag gaacgctgta aaggcctgga tgtcaccatt ttgctgcaag
     8821 attatcgtga cctgaacgac cagtttgatc gtattgtttc tgtggggatg ttcgagcacg
     8881 tcggaccgaa aaattacgat acctattttg cggtggtgga tcgtaatttg aaaccggaag
     8941 gtatattcct gctccatact atcggttcga aaaaaaccga tctgaatgtt gatccctgga
     9001 ttaataaata tatttttccg aacggttgcc tgccctctgt acgccagatt gctcaatcca
     9061 gcgaacccca ctttgtgatg gaagactggc ataacttcgg tgctgattac gatactacgt
     9121 tgatggcgtg gtatgaacga ttcctcgccg catggccaga aattgcggat aactacagtg
     9181 aacgctttaa acggatgttt acctattatc tgaatgcctg cgcaggtgct ttccgcgccc
     9241 gtgatattca gctctggcag gtggtgttct cacgcggtgt tgaaaacggc cttcgagtcg
     9301 ctcgctaaag gctattctat cgccccctct tcgggggcga gttcagatca ggcttctgta
     9361 cctggttgat tcatggcatt ttctcgtgcc gccagcacac gttctaccgt atctaccact
     9421 gcctgagttt gtggatcgat ttcaatgttg acgcgtgcgc caagtttttt cttcccaaga
     9481 gtcgtgcgtt ccagtgtttc cggaattaaa tggacgcaaa aacgcgttgg cgtgacttcg
     9541 ccgacggtca ggctaatacc gtcgatgcca ataaatcctt tgtacaggat atatttcatc
     9601 aactgactat cctggacttt aaaccagatc tggcgattat tttccgaggt taatattttc
     9661 gccacttcag cggtggtcat aatatgacct gacattaagt gtccgccaat ttcatcactg
     9721 aatttcgccg cacgctcaac gtttacccaa tcccccactt ttaaatcgcc aagattggta
     9781 atgcgtaacg tttctttcat caggtcaaaa ctgacatggt tgccgttaat ttccgtcacg
     9841 gtcaggcagc aaccgttatg cgccacggaa gcaccggttt ccaggccgtc cagcatgtgg
     9901 tcgggtaact ccaccacatg cgtacgaaaa tttggtttct cgtcaatcga caccagtttt
     9961 gcggtgccct gtacaatccc cgtaaacata cttacaactc ctgaaatcag ttaagacatt
    10021 ctgttcagca caatagcagg tggaaaacgc ccttaccagt gaaggggtaa gaatggctat
    10081 tttttcactg gagaattaat aaatcctcgc tacaatagac tgaatttccc ctgcttcttc
    10141 tttttgctgc ccattcaggc ggctttttag tctctcatat aactacaaat aaaaggtgtt
    10201 cacgtgcaga agtatatcag tgaagcgcgt ctgttattag cattagcaat cccggtgatt
    10261 ctcgcgcaaa tcgcccaaac tgcgatgggt tttgtcgata ccgtgatggc gggcggctat
    10321 agtgccaccg acatggcggc ggtcgctatc ggtacttcta tctggcttcc ggcgatcctc
    10381 tttggtcacg gactgctgct ggcattaacg ccggttatcg cgcaattaaa tggttccggt
    10441 cgacgtgagc gcattgcgca tcaggtgcga caaggtttct ggctggcagg ttttgtttcc
    10501 gttctcatta tgctggtgct gtggaatgca ggttacatta tccgctacat ggaaaacatc
    10561 gatccggctc tggcggacaa agccgtgggt tatctgcgtg cgttgttgtg gggcgcgccg
    10621 ggatatctgt tcttccaggt tgcccgtaac caatgtgaag gtctggcaaa aaccaagccg
    10681 ggtatggtaa tgggctttat cggcctgctg gtgaacatcc cggtgaacta tatctttatt
    10741 tatggtcatt tcggtatgcc tgagctcggt ggcgttggtt gtggcgtggc tactgcggcg
    10801 gtgtattggg tcatgttcct tgccatggtt tcttacatta aacgcgcccg ctccatgcgc
    10861 gatattcgta acgaaaaagg caccgctaag cccgagcctg cggttatgaa acgactgatt
    10921 caactcggtt tgccgattgc gctggcactg ttctttgaag tgacactgtt tgccgtcgtg
    10981 gctctgttag tgtctccgct cggtattgtt gatgtcgcag gacaccagat tgccctgaac
    11041 tttagttcac tgatgtttgt gcttccaatg tcgctggcgg cagcggtaac tatccgcgta
    11101 ggttatcgtc tgggtcaggg ctcaacgctg gatgcgcaaa ccgctgcgcg gaccgggctt
    11161 atggtgggtg tctgtatggc aaccctgacg gccattttca cggtttcact gcgggagcaa
    11221 atcgccctgt tgtacaacga caatcccgag gttgtaacgc tggctgcgca tttgatgttg
    11281 ctggcggcgg tatatcagat ttctgactca atccaggtga ttggcagtgg gattttgcgt
    11341 ggttataaag atacgcgttc cattttctat atcaccttta cagcttactg ggtgctgggc
    11401 ttgccaagcg gctatattct ggcactgacc gatctggtcg ttgaacctat ggggccagca
    11461 ggcttctgga taggctttat tatcggcctg acatcggcag ccattatgat gatgctgcgt
    11521 atgcggttcc tgcaacgtat gccgtcagcc atcattctgc aacgagcgtc ccgctaataa
    11581 agacaaggcg caaccttcac gggttgcgcc tgtgttttta cgcaggctgg agcgttgcgc
    11641 caatcccgtc ttcgtctggc tgtaatttca gagcgttaca cagagtttgc cgactggcat
    11701 ctgttatctc aacgcgtata tccccgttaa cttccccttt cacattgatg aagtcaggtg
    11761 aaatttcttt cgcactggtt tgcaggcaaa tcgctccctc tccgcgcagg cgtttcactt
    11821 tcagttgcac atgcgttgcc gcaccagtga tcagccacag ttttccgcca ttcagattga
    11881 tgttaccaac aatgaaagtg acgctgtcac cttttaccgt actgccgtcg agaatcaaca
    11941 caccgccctg ctgtacagta atcgttgatt gttgcagctc tgcgccataa ccaataatca
    12001 ttgaagcgcc gtccagtaat gcaacactcc cggtcagttc aaccggtgcg cgatcttcct
    12061 ctacgacaaa attttcgtcc gcgctggtgg gtgacaacag gatcactgtt ccaccttgta
    12121 ttaccgcatt ttccagcgta ccggcatgca cttcagcccg cccccccaca ttcaccgaca
    12181 ggttttgcgt cttaccggaa ctgtagagct gaaggccatc cgtccccaga cgataaatgg
    12241 atccattctc aacaatagtg tcagagtctt caccaccgag attattcatg gtgccaccag
    12301 caagcaacgt atcactggct ttcccggcca gcataaaaac gccgccctga ttaatctcac
    12361 aattgctggc ttctccactt ggatacacca acaattcacc accttcatct accacgaccg
    12421 ctgaggttgt cccattaacc aacaggccgc cctcttgatc gagaatgatt ttttccgcgc
    12481 gatgtccttc cagtacacgc aggttaccgc cattttccag caacaaaccg caggcataac
    12541 cctgatcaac gctgaattca ccttcgggat ggcggccatt aaccgtagcg agcgtagaga
    12601 gactaattgc gcctccctcg gactgaacaa tatctgtggc aatggcaccg tcgctgaccg
    12661 atagcgtgcc attgttaccg tactgtgtgc atttggcgat cccaccggtt gccactttca
    12721 accagccagc ccccgtgacc acggtatttt cggctcttcc gccagcttca acaatcaact
    12781 cgccttcagt aagttgcgtc gcgccaagga cctcgccggt cttaacaatt tgcacattcc
    12841 cgtcgctcat caagtttttc gcatcagatc ccatgattta ttcctttgct gcatctgtgt
    12901 gcctttattg ctacctaagt gtaaaggcta cggaggattt atccacgaca gatttgagat
    12961 ggtggcaaac aactctgttt aaactctgat acacgaatta ttgggttgta tcagatgtaa
    13021 atgcgatcct gaataaaaat cgcccttgca aatcaacaaa atatagccaa tttgcttaaa
    13081 agctctgcaa acggtcgaaa tagcgcagaa aattacgttt tgcctcttgc cacctttcca
    13141 ctctgccgct aatattcgtc cccgttgtca cctacaacgt tgcgttcata gctcagttgg
    13201 ttagagcacc accttgacat ggtgggggtc gttggttcga gtccaattga acgcaccatc
    13261 ctgcgtccgt agctcagttg gttagagcac caccttgaca tggtgggggt cggtggttcg
    13321 agtccactcg gacgcaccag attttcttaa tctggtcttt tcctttttcc ctctgtttct
    13381 tctctgtatc caatacgtta aaagatttac actgtcttcg tatgcgttat cagaaggaga
    13441 atcgctatgg caactttgtt acaacttcat tttgctttta atggcccatt tggcgacgca
    13501 atggctgagc agcttaaacc acttgctgag tcgattaatc aggaacctgg ttttctgtgg
    13561 aaggtatgga cagaaagtga aaagaaccac gaagccggtg ggatctacct tttcactgat
    13621 gaaaaaagcg cccttgccta tctggaaaaa catactgccc gactgaaaaa tctcggcgtt
    13681 gaggaagttg tcgccaaagt tttcgatgtc aatgagccac ttagtcaaat caatcaggca
    13741 aaactcgcct gacagaattt aatcaagggc ggttagcgcc cttttcatcc ctgtctgaaa
    13801 tttctcaaat tctaaaaatc tcaaccaaac ttatctgata acactaaatt cgaaagaatg
    13861 cgtacaggta agtaacaatg aaaaaaattg ctattgtggg tgccgggcct acggggatct
    13921 acaccttatt ctcgcttcta cagcaacaaa ctccactttc tatttctatc ttcgagcagg
    13981 ctgacgaggc cggtgtcggg atgccataca gtgatgagga aaactcaaaa atgatgctgg
    14041 caaatattgc cagtattgaa ataccgccga ttaattgtac gtatctcgaa tggctacaaa
    14101 agcaagaagc cagccatctc cagcgttatg gcgttaaaaa agaaaccttg cacgatcgtc
    14161 agtttttacc gcgaattctg ctgggcgaat atttccgcga tcaattttta cgattagtag
    14221 accaggcacg acagcaaaaa tttgcagtgg ctgtttatga atcatgccag gttaccgatc
    14281 tgcaaattac aaatgctggc gtcatgctcg ctacaaatca ggatttaccc agcgagacgt
    14341 ttgatttagc ggtgattgcc acgggtcacg tctggcctga tgaagaagaa gcaaccagaa
    14401 cgtattttcc cagcccgtgg tcaggcctga tggaagcaaa ggtcgatgcg tgtaacgtgg
    14461 gtattatggg aacatccttg agcggactgg atgcggcaat ggcagtggct attcagcatg
    14521 gttcgttcat tgaagatgat aaacaacacg tcgtttttca ccgcgataac gcaagtgaaa
    14581 agctaaatat tacgttgatg tcgcgcacgg gtattttacc cgaagccgat ttctattgcc
    14641 ctattcccta cgagccctta cacatcgtca ccgatcaggc attaaatgct gagattcaaa
    14701 aaggtgaaga gggccttttg gatcgggtat ttagattgat agtagaggaa atcaagtttg
    14761 ctgatccaga ctggagccaa cgcatagcct tagagagcct gaatgtcgat tcctttgctc
    14821 aagcctggtt tgccgagcgc aaacaacgcg acccatttga ctgggcagaa aaaaatctcc
    14881 aggaagtcga acgcaataaa cgagaaaaac atactgttcc ctggcgttat gtcattctgc
    14941 gcctgcatga agccgtacag gaaattgttc ctcatctgaa tgaacacgac cataaacggt
    15001 tcagtaaagg ccttgccagg gttttcatcg ataattatgc ggcaatccct tcagagtcta
    15061 ttcgtcgact actggcctta cgtgaagcgg ggatctgaaa tccctgatgt tggcgaagat
    15121 tatacgttac agcaacccga agatattcgt ggacgcgtag cgttcggcgc gttgccctgg
    15181 ttgatgcacg accagccttt cgttcaggga cttacggcat gtgcagaaat tggtgaggcg
    15241 atggctcggg cggtcgtaaa gcctgcatcc cgtgctcgtc ggcgtctttc gtttgattaa
    15301 aggtaaagct atcagcactc aacaaactga tgctgttcat taagacgata gcggactccg
    15361 gttcgaatat tgttttcacc ttcaatggcg acggcgaagc gaactctttc gccgtcatga
    15421 tatgccagtg cggcgcttcc ccctagcccg agaataattg agtcgaccac gcccgtgctc
    15481 actaccgttg aattttcacc gctggcaatg atgtgtacat tgtcgccgct gttggcaatt
    15541 ctggcgtttg cgccaaagct ggcgatttgc accaggtcac cattactggc gacatgacac
    15601 cgttcaccca atgtacaaac gcgtactcgc attccggtat tcgcaatccg actactatct
    15661 ccagcgctgc ttatccgcga agaattaccg gttacagcaa cccgtgcgcg ttcaccggag
    15721 ctgccaatgt gactgttaaa gccgacactt ccaatccgca cgctataacc catactggca
    15781 atttgcgcgg catacccagc ggttgcgatt cgggcgttat cctcacaaca gccgatgcga
    15841 gcatgatcgt cgctgcgggc aaactgactg caaaatggta tctctccggg gtctgtggcc
    15901 agtttttgca tagaggaaac ttcctgatgc gcgaagtttt catccgccag ccattttttc
    15961 cacgcatact caaccagact ctccgcccag tccgtatatc cttcctcaat cagtgcatga
    16021 cgaatatcgg cataactacc gccgcgtggg aaatggcgca ggaaccagcg atacatcacc
    16081 gctccgatac gccattctcg caggtctgct cgtgtaatga tcattcgtta gtgccggtga
    16141 tagccatcga ccatgctttt aaaggtttcg tgtggcgtac gccccgtggt gcaaagataa
    16201 agatgaccga agataaaaaa gaggcttata aatgccagag caaaatgcgc ctgcaataac
    16261 cagtatctta cgccaggaaa cacatctccc acggcttgcg gatagagaca caacagcccc
    16321 gtcaatagta acaacggcag caatccatac atgacaccaa cataggcgac ctgctgtaag
    16381 ggattaaatt tagactgtgt tgttgccggg aaaggatgtt cttccccctg cataatgcca
    16441 aacaaataaa atcgcgtttg ttttgccgct cgttccagcc acccctgacg acgaatgcga
    16501 tagtggtgac cattatcccc aacggcattg atcagcacaa agccgagcca gcatgccagt
    16561 aacaaaaatc cgcaaacttc atgcaccgca accagacttt taaccgcagt tgcgcccacc
    16621 atcgcaaagt gatttatcag cccacttgcc agcaacaata caaagagtaa cgcattcgac
    16681 cagtgccata atctgaccgc tttgctgtaa agataaacct tctcaccatg atcggtggct
    16741 gatttcttca cgccacgagc acgaagcaag gcgtgcagcc ccagcaccaa ccacatccca
    16801 accagcaata ctccggcaat gatcaaccac accggccaaa attcgggagt gaactgtggc
    16861 acatagttcg ctaactggct ctgaaactgt tcagcatgtt gcgacgggtt catacatttt
    16921 cctttttaat caaatgttga ccgaaccggc gatacagatg cggttttccg gcaccaggta
    16981 gctgatattg atagtattta ttatcctgca accacgcctg aatttctggg ctatcttcgc
    17041 gaccaaagat caatgcgtgt tccggacagg cgctgacgca aatgggcgga aagccttttg
    17101 ccagtcggga ctcagcgcag aaatcacatt tatctgccac tttagtgaca gggttaaggt
    17161 agcgaaccag gtacggacaa gcgccaatgc aataactgca gccgatgcac tgcgattttt
    17221 ctacccgcac gatcccctgc tcatcacgcc acgatgcccc cgtcgggcat acgtcgatgc
    17281 acggagcatc ttcgcagtgc tgacatgact ggcgaaaaaa gtgatactgc gtttcgttgt
    17341 cgttatcggt gactggaatg tgtgctatcg ataaacggct tccctgagcg gggacgtggt
    17401 tagttttacg gcatgcgcgg gcgcaaatat tacagccatt gcaccgtgac tcgtcatgaa
    17461 tcatggcata ccgaacttcc ttttcttgcc tcgtgttcgc taacagagaa gacgcagaac
    17521 cagtaaaaaa gattactgtt cccatcccca gaacaaattt gcgtcgagtg aaggacatct
    17581 gattatcccc taatgcagtg gctgcaactg tccaccctat ccatgacatc tccctgaaat
    17641 ttcaggggtt ctgaaactgc tctacctcga accattcgca taagccataa gccttgccat
    17701 aggtcataaa tcgctcgcta aacaagtaca acaccgccgt cgatgactcc acctgccgta
    17761 tatcggcata ttcaggtgca acatccatcg cggcgatcgc ggcttcaatc tgcgcttcct
    17821 ggaagtaata tggcgcttgc atcagcatgg cgactttgta aggacgagga tacgtctggc
    17881 actcaaaacg aacggcgtgt gcaatggcgc gacaaatgtc ctgctccaca acttgcaaag
    17941 acatcgccgc gtagttttca ctcatggcct gcgttgagta atagtaatca tcctgacttc
    18001 ccgtcataca agcgatatcc gcgtagtctc cgttctgctt aatgctttcc agcaaaactg
    18061 acagttcctc ttctgcaacc gaatagggtg gcgagagaaa cacggtgcgc gccaccagct
    18121 gcccagccgc agaatgctgg cgaatgaact cagccaggat ttcagcaggt tctggttcgg
    18181 ttaccgtatc aacggggtga cgcatcccct gcaaccattg ccgcttcgcc tcgtcaactt
    18241 cgctgacttt cgctaacggc aattcatcct gatggttcat cttacccatt ccttacgcag
    18301 gcagtagatt gtgtgctgcc agatcggcgg caatatcttc cagccccaga cgctgcaatg
    18361 tttcgcgggt tggacaacca agctgtgggt cccagcccat ttctttgtag aacatcgtga
    18421 gcgaagcatg catatcgtca cgatccattt tgtcagtacc ttcagtaaag accgggatct
    18481 gcggatcctt gtcgaaaacc caggaacaga taagatcgtg ttcattacgc atatctttgg
    18541 tttgcatcgg ttttaccgtg taggcacgat gcaacgtaaa gatacgctct gcagctaaat
    18601 ctaatttttc ctgagtcatc tcttcgccgg tgatcgcttt gaagaatttg gcttcaagcg
    18661 ccagatcgcc ccggtaatta cggcttttca gcggcgaaac ggtcattggc cagacccagt
    18721 tgcacagagt gacggcgtta tgcaaacaga cccgcaacag cgaccacttt gcatatttaa
    18781 tttttgcgtc attgattggc gtgtagtttt tggtttcatc gtaagcatct tcagaaccaa
    18841 aaagttcttt cgccacttca cgttgcagtt tcaatggcaa gccggaacca ataaagttga
    18901 tatgggtatg cgtcatgcag tcacggttga acatacactt aacaatggaa ccgacctgcg
    18961 ctgacgcttc attggcatgg tgaaccggat agccaaacgg cgaccagagt ttatttttcg
    19021 cgtagcccca gtactcttta cccaaattcc agcgttctgc gatggcatat gaaccatcag
    19081 ccaggtgact cagctcaccc acacgatgcg ccagacggta gtaaaaatct ttaatgaagt
    19141 taacgtcacc cgcttccagt tgatcccagc gaatttctgc atactcttca gctggcagaa
    19201 cacgcttgaa cacacctttg ctgtaacaat aagtaaagtc gcgatgcaac tgcccgtagt
    19261 tacaccatag gccgtagtca tcgaacaaat tcagaccaac caggttacca atcacacgac
    19321 catcgtcttt atcttcaaaa tctttcggcc cgttcgggaa gatggtggta tggacaaagt
    19381 ttgctacaca agtgttacca cctgtgctgg gcacgccaaa ctctttcacc cgaggaatat
    19441 tcatttgggt catacaacgg atcgggcaag agtggcagcc gctcattttt actgtgtatt
    19501 tctctgccgc cggtcctaag tcaaaaacgg atttataggt acgaaagccg accgtattct
    19561 gattgcctgg cggaatttca cccgtttcaa tcggaccacc ttcagccgcg ccccaaaaca
    19621 gccctttacg tgctgtccag cgtgacttgg gatctgaata ctctgcccac gattgtggcg
    19681 tacttggcac gacatggttg ttattcgcac caataagttc agtcatcatg taatcattga
    19741 gacgcttcat ctcctgacga tcggcaatgt tgaccccttt cgtcccttca accgcaatcg
    19801 ctttcaggtt tttcgaaccc attattgcgc cagttcccgc accgccgctg tggttacggc
    19861 tattcaacat gccagagaga ggaataaggt tttccccagc ctgaccaata gccgccacac
    19921 aggtttccgg gctggtcaat cgacaaattt cttccgtcgt tgcgcgcgtc ccttttcccc
    19981 ataagaaatc ggctttttcc aggctaactt tgtcatcttt aatcttcagc cataccggtg
    20041 atttcgcctt cccttcgata ataatgacgt cgtatccagc gaatttcatt tgcgctgcaa
    20101 aaaagccacc catatgggca tcgcgactaa atttcctttg gtaaaagtag aaagtgaggt
    20161 gatatttacg cgagaactac agggggcacc agatccaggt aatgggccgg tagcaaagac
    20221 taatttattc gcttcatcga aaggtttcgt gcctagcggt acttcgtcat acataatttt
    20281 gtagccgaag cccatgccac cgacaaaact tttaaactta ctggaatctt cgagggtaat
    20341 atttcctgtc gtgagattga ctcttaatat attacctgtc caaccgttag ccatgatttt
    20401 ttcctttgca agattcacac agtaatatct ttccactcga taatttttaa cacccctgtt
    20461 gggcaggcgt ttgcgcattc accgcataac acacatttcg aggatttttt actttcggta
    20521 tttacggtgg ccatcatcca cgggcatgcc gtagtacagg cgctacagcc aatacaacgt
    20581 ttatgatcga cggtaataca gccttctttc tgctgccagg taatcgcacc aatcgggcag
    20641 acgttcatgc attgcggctc tttgcattga cggcaggtgt ccgcggtata gttgagatcg
    20701 ccatacaggc cgccgccaga gccaaccccg ttgtcgccaa agaaataatt gcgatggatt
    20761 ttgatacggg agaagaatgt ccctactgag ccatcgttga agttggtaca agagatttca
    20821 cagcggtggc aacccgtgca tcgcgctcgc tgggtcacca gcaccccttt gggtgtattt
    20881 atcaacccta ctgtgccgct atcaatatct tcctgtttgc aacccaaaag tgacagtaac
    20941 gcaggggcaa ttgttagacc agcaaggcct tttcctgata tacgcaagaa ttctaatcgc
    21001 gttaagccaa tatctaatag tggacgatca accgggttca tttatttcgc atcctcttcg
    21061 caaacacagt gctgacaatt cagcagcctg atattaagca ttcaactaat acgctgctga
    21121 ctctgtcccc aacaccagat attttcccta acccgatagt gtatgagaaa agatttaacg
    21181 ccgataaact aatcctttat ggttaggaga aaaaataatg tgattattct gagcccttaa
    21241 cattgatcgt tatcaattaa aattacaaag caagaaatta taagcgaact gatatttatt
    21301 atcatttgaa ataaatttaa cttaagcgac aatcatttgt gacgttcatt gcagaaaata
    21361 aaaataacat tatcgctata aattaaaata cataccgaaa ttcgtataat ttcagcagtt
    21421 gttatttaga atgattaact ggaccgcaac tgaagcaaga atatcaggat gaagcttacc
    21481 gcctcatcct gaatggtcat tttaccagtg cgttaatgac ggtttccatc gcctgctttt
    21541 ccagctcact gcgctgaatg cgtttattgg ctaatgctgt acgtaatacg cctgcgataa
    21601 caatatgctt tggttcctgc cccagatcgc gcatttccag cacaacttta ccgacaactc
    21661 tgcacatttc tcggtacagt tcatcctctt ttgtccgatt tcccattctt tatacctatt
    21721 tatcatgcca a
//
LOCUS       DAJTSI010000061        21564 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000061 DAJTSI010000000
VERSION     DAJTSI010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.061"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..695
                     /gene="hemF"
                     /locus_tag="OO327_002074"
     CDS             <1..695
                     /gene="hemF"
                     /locus_tag="OO327_002074"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR6018616.1"
                     /translation="MPASATAHRPELAGRSFEAMGVSLVVHPHNPYVPTSHANVRFFI
                     AEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVYPRYKKWCDDYF
                     YLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVERRKAMAYGERE
                     RNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEYDYQPKDGSPEA
                     ALSEFIKVRDWV"
     gene            complement(701..1753)
                     /gene="eutR"
                     /locus_tag="OO327_002075"
                     /pseudo
     CDS             complement(701..1753)
                     /gene="eutR"
                     /locus_tag="OO327_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(1799..2299)
                     /gene="eutK"
                     /locus_tag="OO327_002076"
     CDS             complement(1799..2299)
                     /gene="eutK"
                     /locus_tag="OO327_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR6018617.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(2312..2971)
                     /gene="eutL"
                     /locus_tag="OO327_002077"
     CDS             complement(2312..2971)
                     /gene="eutL"
                     /locus_tag="OO327_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR6018618.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            4153..4944
                     /locus_tag="OO327_002078"
     CDS             4153..4944
                     /locus_tag="OO327_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018619.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            5664..6059
                     /locus_tag="OO327_002079"
     CDS             5664..6059
                     /locus_tag="OO327_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6018620.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            6077..6340
                     /locus_tag="OO327_002080"
     CDS             6077..6340
                     /locus_tag="OO327_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018621.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            6363..6905
                     /locus_tag="OO327_002081"
     CDS             6363..6905
                     /locus_tag="OO327_002081"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018622.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            7044..7331
                     /locus_tag="OO327_002082"
     CDS             7044..7331
                     /locus_tag="OO327_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018623.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            7370..7576
                     /locus_tag="OO327_002083"
     CDS             7370..7576
                     /locus_tag="OO327_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018624.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            7888..8373
                     /locus_tag="OO327_002084"
     CDS             7888..8373
                     /locus_tag="OO327_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6018625.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            8354..8632
                     /locus_tag="OO327_002085"
     CDS             8354..8632
                     /locus_tag="OO327_002085"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018626.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            8625..10085
                     /locus_tag="OO327_002086"
     CDS             8625..10085
                     /locus_tag="OO327_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6018627.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            10119..10928
                     /gene="murI"
                     /locus_tag="OO327_002087"
     CDS             10119..10928
                     /gene="murI"
                     /locus_tag="OO327_002087"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR6018628.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(11047..11741)
                     /locus_tag="OO327_002088"
     CDS             complement(join(11047..11495,11495..11741))
                     /locus_tag="OO327_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6018629.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(12260..13633)
                     /gene="eutA"
                     /locus_tag="OO327_002089"
     CDS             complement(12260..13633)
                     /gene="eutA"
                     /locus_tag="OO327_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR6018630.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(13608..14087)
                     /gene="eutP"
                     /locus_tag="OO327_002090"
     CDS             complement(13608..14087)
                     /gene="eutP"
                     /locus_tag="OO327_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR6018631.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(14100..14435)
                     /gene="eutS"
                     /locus_tag="OO327_002091"
     CDS             complement(14100..14435)
                     /gene="eutS"
                     /locus_tag="OO327_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR6018632.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(14728..17007)
                     /gene="maeB"
                     /locus_tag="OO327_002092"
     CDS             complement(14728..17007)
                     /gene="maeB"
                     /locus_tag="OO327_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR6018633.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            17296..18246
                     /gene="tal"
                     /locus_tag="OO327_002093"
     CDS             17296..18246
                     /gene="tal"
                     /locus_tag="OO327_002093"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6018634.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            18266..20269
                     /gene="tkt"
                     /locus_tag="OO327_002094"
     CDS             18266..20269
                     /gene="tkt"
                     /locus_tag="OO327_002094"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6018635.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(20372..21415)
                     /locus_tag="OO327_002095"
     CDS             complement(20372..21415)
                     /locus_tag="OO327_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR6018636.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
BASE COUNT         5661 a         5314 c         5193 g         5396 t
ORIGIN      
        1 cgatgcctgc ttccgccacc gctcatcgcc cggaacttgc cgggcgcagt ttcgaggcga
       61 tgggcgtttc actggtagtg catccgcata acccgtatgt tcccaccagc cacgcgaatg
      121 tgcgtttttt tattgccgaa aaaccgggtg cagagcccgt ttggtggttt ggcggcggct
      181 tcgatttaac ccctttctat ggttttgaag aagacgccat tcactggcac cgcaccgccc
      241 gtgacctgtg ccagccattt ggtgaagacg tttatccccg ttacaaaaag tggtgcgacg
      301 attacttcta cctcaaacat cgcaacgaac agcgcggcat tggcgggctg ttctttgatg
      361 atctgaacac gccagatttc gaccactgtt ttgcctttat gcaggcggta ggcaaaggct
      421 acaccgacgc ttatttacca attgtagagc gacgtaaagc gatggcctac ggcgagcgcg
      481 agcgcaattt tcagctctac cgtcgcggtc gttatgtcga gttcaatctg gtctgggatc
      541 gcggcacgct gtttggcctg caaactggcg ggcgcaccga gtcgatcctg atgtcaatgc
      601 cgccactggt acgctgggag tatgattatc agccaaaaga tggcagccca gaagcggcgt
      661 taagtgagtt tattaaggtc agggattggg tgtaactccc tcacccccat tcccgcatcc
      721 gctgatgcaa cgtcaacgac ggcttctcgg caaacagctg ctggtaatcc gtggcaaatt
      781 gccccagatg ccagaatccc cactgcatgg cggcgtcttt taccgttgtg ctttgcgacc
      841 acgggcttat cagttcgcgg cgtacgacgt tcaggcgaat gcgtttcagc cacgcgtttg
      901 ggccaatgcc taaaatagcg tgaaacgcgt tttgtagcgt gcggcgactg acatgcagtt
      961 gattacacaa atccagcacc gtcaccggtt cggacatgtt ttccagcaca tattcacggg
     1021 cgcgggaaag caaccgacgg taactctgat gactgatgct ttccgccgtc accattggtt
     1081 gcgcttcttc cagcatcgcc cccatcgcca ttagcaaatt atcccccagc acttttcgca
     1141 ctgcaggctg atggagattt tccgggttct cgcaaaacgt cgccagcgcc tgttggacaa
     1201 agccccacag cgcggcttta tgctgctctt tcacttccag cgccgactgg ctacgcagca
     1261 tatgtaatac ccgatccgga ttgtgcaaaa agttagcctg acaggtgatg acatcttcag
     1321 acagcactac gcccaggatc gtataatcgt ccggcgtact taattcaaac tcggttccac
     1381 cggggcgcgt cgctatttct gcgcttccca gacattgcga accgataaat ccctgctcac
     1441 cgcgcgtcgc cggaatgcca aaccagaacg agttcggcca gaccaggcac gactgacgca
     1501 gcgccagacc ggtgtattca cgaaaaacct gaatatcatc gagtagaatt tccgtaaatt
     1561 caccatgaaa cttgcccgga tgcagctgat cgtaaatctg ctgctaggcg gtaatcgtca
     1621 aagcatgttc atagacatcc gttgtctgtc gttgatgaac attatccact tcgaccttcg
     1681 gcgtgagctt caggttttcg ggtaagggtt catgataaag atggtgcaaa ttggctgtac
     1741 gggtcttttt catgatgtta atgccgggtg ttgtaggaca cccggcacct ccgacaggtt
     1801 aatggggctt gagacgataa cgactactgc gtttacgtaa cgtcccggca gaaaagagct
     1861 gttcgagcgc atttctggct ttttccagcg gccagccaaa gtgggcggcc acttctcctg
     1921 ccgtcattcc ctgacatact gatgttaaca gcgccaacaa ctcgtcagca gattccgcga
     1981 cgataactgg cgcgtcgggt tcctttacgg gttgcttcgg ctggcggtta aagccagtaa
     2041 ccagccactg ggtgtcatcg tctggccgac cgatctcctt acggctgatg acacgcccgg
     2101 tacgcatcgc ggcagcacaa cctgcgtcca gcgccgcacg acacgccgcc agatcgcctt
     2161 ccaccaccag cgttaagcga ccagggtcaa gcacttcgtg actgagcaga cgaacgttag
     2221 ctgctttgag catggcatcc gctgcatcta tcgcggcgac catgccgtcc acttccagca
     2281 atcccagtgc attgatcatc ggcaacctcc gttacgcacg ctggattgga ttacgcgcga
     2341 tttccagcac tgcatcggta aaggcgttac aggctgcttt acacgcggcc tggctaccgg
     2401 ttaaaaatgc tgccgagtag ttggtttcag acggtggcgg gacgtaggtc accagctgta
     2461 cgtcggcaga tttcaacgcg gcatcaatgc cgtaggtcgc ttccagcggc ggtgccacca
     2521 gatacgccat cggatcgcca agcgtgatcc cggcggttga tgaaagataa gaaccggtac
     2581 gcgaaaccac atgcgccagg aaggcggtgt tttctgcgtc attggcccac tggaaagctg
     2641 cgccattttc aatatgtgca accatcgcat ccagaccggc acgcacttcc gccgggtttg
     2701 gcccaccaag cataatcagc acttcaccgg cagtcggtga cgggccgtgt gccgctcccg
     2761 catacagcga gcgaccatat accacttcga ccatcgcttg tttggtcgct tcgtcagccg
     2821 caatgtaggt tacgtcgtca gaatcggcag aaatcagccc gaggctacga atatgcggcg
     2881 gcaattttag ttcacgcgca aattcagcgt taacggaggc aatcacccgc atggcggtta
     2941 ccgacggtcg aatcaaatct aaagctggca tgatgcctcc ttatcgggtc atgttgatgc
     3001 cggatgcttt ctgctccagc atacgtttcg ccaaatccac aatgacggca cagcagcacg
     3061 aaagagaagc aaaagtcagc tacctggtac actaggcagc gggtcagcaa aggggggcaa
     3121 atgccccttt tttcatgtca gataatcaga tcattacctc aataaactaa taatcacatc
     3181 gcggttacaa ccagtttgat agtgatagct caaacccaga cgtggcgctg gctgcaagca
     3241 aaataagccg caaaaatgcg accccatgtt tactagccgc aaaaatgcgg tcctatttgc
     3301 cagaaccttt tattattcaa ttggttagtg caagaaaaag cgtgattact gttcaagaag
     3361 tatccagaaa ggtcatgatt ttgacgctga aaaaaaatag gtgggcattt ttgcccactc
     3421 actagccgca aatttgccca ctcactagcc gcatttttgc ggcccaaagt aataaataca
     3481 gagaaataat aaatacagat cagcgccaag atttttcgtc ttttcggcgg aatcatgaga
     3541 ctagtgctta cgtcctgata acctccagag cagaccttaa cttctgtgca gcaggactac
     3601 gctcattgcc gctcggctag gtcaaatcct cacgataaag ctgttcggat tgtgaccata
     3661 atgttaaagg actctttaaa tcattccttg acgaattaac tagctggctt aagcggaata
     3721 gcatgcagga taaaaacaaa accagcttag cagtggggag agcttcgctt cgctcgtctc
     3781 ctggggccaa tgctcaatga aacgacgtaa ggcacggagc gctcaccata ggtagacaca
     3841 taaacatctg agagcgcacc ttctatcgcg ctacgcgctc aataaaaacc aacgacaccg
     3901 ccacactgca agaccaatgg tttttatcgc gtatacagac cagacaggaa acagcagcaa
     3961 agcagcgtat agccaggtgc agacagcaca ctcacacgac gcacagcagg cttaagtgat
     4021 cactcaggaa actttatccc cgccgccgat caccgcgaac tatcatgacc gcgccaaatt
     4081 cgaaaggaat ggcccataga acgcgatgaa cacacataag cgctacactt acttaacgtc
     4141 gccacagagc gattgagagc gcttaaaatc gatgcgattt cattaaattt ttctcactcg
     4201 cctgaatggt ggcgatcagg actgatcgca acattgcccc gcctcgatat tgagcgcata
     4261 atagatacat acattaagcg gaaaagagat aagtttattg tgaaaatcag ggggttacat
     4321 atcatgcgca tatcagaaca acaacggcag atcctcgact atctggcaaa aaacgaatca
     4381 atcaccgcac gacaagccgc agatctcgtt tatggtggca atgttacccg cgtccaggtt
     4441 gacgccgccc gccgctcact catgacgatg gttagtaatg ggctaatcag gaaacaaggc
     4501 cgcgctttcg tagctgaaag acatatcaat cacgcttata gccaggacgt tgaagaattg
     4561 cgccgccaga tcctttgggt attcagcaaa cagggtcagg agtatttcct ttctgaacac
     4621 ccaaatttcg ccgccaaaga gttaacagta agcggattgt cgtgcgcact ggattatctt
     4681 gtcggtgaag gttgcgttac ttacaagcag ctatggcacg ccctgaatac gcttgtagaa
     4741 gatggtttac tcattgccac gcgtgagaac atcgaatact ggataatgcc gcgttcaggt
     4801 cgccgcaatc atgcacggta tatttcaggc aacagataca gaacgccgga ctttaatccg
     4861 acacaggaag atcgggaccg tgccgaaatg atgtgcaggg aacaggagaa aagaattatg
     4921 gataagttat tttcgatgca gtgaacacaa gcgggcgcaa tgcccgtttt tattgcctta
     4981 ttccacgcct gacgaatcaa tttgatcaca cctgagaaaa tgcatagcca gaagttaagg
     5041 ccgttatatt gcgattggga gcgttttaaa cggtatcaat ttaattaatt ctgatgtggt
     5101 gatttgcagg caaaaaaaac ccgccggaga agcgggaaat agagacatat cgttgtagtt
     5161 attaacagat cactttgagg tcgagggcgg ctaaccctcg ctaatattat cccgctatta
     5221 ccttgcgggt gcaaataatc ttttattgct ttgatgattt aataaatagc actttttgct
     5281 aacaaaaacc gctgtcaata tatggccaaa aatgtcaaca gaaaaaataa ggtttataat
     5341 attaagtatc acatgggtaa tgggttcagg gaatcggaat ccaccgcctc acggaaattc
     5401 aaaatttgct tcttctttcc tccgtgggcg gcttcttttt cgtatcgagg tatttatgaa
     5461 agcaataaca ttagaacagt tcgcagcaat ggcgaatgta tcgaccagaa cgctgaggcg
     5521 ctggctaaaa aaaccatcaa ccggagtccc tttgccattt accccacgcg gtactcgcca
     5581 attattctgg gagcacgaat gcaaagaatg gttgtacaga acagaacgat aataatcacg
     5641 catcacattt tgaggtaaac atcatgacca atatcgaaag attagaacgc tgtgccgata
     5701 ttatgcgacg cagatggatt tatgaccctg aagcgggttt attgtttagc cgtgaaacaa
     5761 aagacgttat caaaggcagc atgaccaata aagggcattt ggttgtaacg gtacatgaag
     5821 gtaaattcaa agtgacactc acctatcaga aagcctgcta tgtatacaca tacggcgctt
     5881 atgacgagac attatatgaa gtgcaccacg ttaatttgaa caaacaggat aaccgcatca
     5941 agaatattcg cctgatgccg aaagaaaagc accgccggat acataaaaac gtccgcaaac
     6001 ttattcggct gggccacaca caatggcttc aaccgcaatt aattgaacaa tcactataaa
     6061 cacaggagta aataccatgc attcattaga cagttatttt cagcgcacga cagcccctaa
     6121 atcagccgcc caggaacgcc gtgaggaatt ccatgagaaa gtgatgcgtt ctgctgatta
     6181 tattgcggac aagttcgtgg aaacggttcg tccacttgtt gatgaagtag ctgataaact
     6241 tcagtctgaa atgccggaag atatggaagg cactgcaaag cggcgactca tttgcgaatt
     6301 aagtcgtcgt tttggcgtgt caataagcgc ttttaaataa tcgaaaaaag gggggaaaga
     6361 taatggacgc atttgaggta ctggcaaata aaggcgctga actggtagca cagcgtgata
     6421 aaaccgccaa cgaaggggaa cgcgcactgc taaataaaca gattaaagcg atccgtatgg
     6481 cccagtttaa attaatcagt aatgaagtta tcgaaacaat taaaccgcag atcccacaaa
     6541 tcgttgctga cgctattaaa gccgccggac tggagaaacg cattgccggg ctaaaaacga
     6601 acgcagagaa aatggagatg tacaaacaga gcggtgctcc gaatcccgat gagtacttct
     6661 caccggatgt tgagtttatg gcgcaggttg aggaacgtct gggtagctgt ctcaccgagg
     6721 aacagcgtcg ctattttgat ggagtggata gttcggcagg tattgacctg aactcgtatt
     6781 tcggtcgtga aattgagcat tttgatgccg ccccgtctat gccggagcca gaaccagaaa
     6841 tggccaccga tcctgaagct gagcaacgga aacgcgtatt caatgcgata gggattcggt
     6901 actgatcgga taaaagaaag ccgggaatta ccccggctga cttaacaatg tgaccaatga
     6961 acaaatcgga caagacaaac aggataagca gtctcgaaac aatgttcggg aactatatta
     7021 gcacaagtcc ggagaaaatc atcatgttgg cactacgcat catgcagggc atcgctaaga
     7081 ccctcgcaga acatgtctta gacctgaaac attcaccact gagcaaacag gccatgaagc
     7141 gccaaacgct tcgtctgtgg gctgaatatt cgcttggaac tatcaataaa atcatcgaca
     7201 tgaaatcggg gccgagcaac cagagcgcgg aggaaatgga gttcatcagg cgtttaattc
     7261 tcattcgcag agacattcat agccagttac acagcgtggg aatcgatatt aatgatggga
     7321 cgggcgatta attttcggac aagtactatg cgcgcgcgag gagctgaaca tgaagcaaga
     7381 tattgcagac aggctggaga tactcgaagg ccagcgggca gaagcgaaac aactacgtaa
     7441 gcaggccaga agagcgcaca ggaacaacga agcagagtta ctaaccaaat acatcagttt
     7501 caccaattac tgcatctacg agtgttacaa ggaagatgca gaggactggc tggattcatt
     7561 gccagaacag tactgagcag aataacacca cgtcatgttg ttctgaggtg agcatatcac
     7621 ctctgttcag gtggccaaat ttagtgtgcc acttcagcat caccgttgaa atcacaataa
     7681 cgattttttt gtaaatatgc tctcacatat gtaggaatag accaggttgg cattgaaaca
     7741 catctaccat gaccataata aagtaatttt ttgatatgtt tatggtttta tattaattaa
     7801 aatatatcta aatttgatag ttttaaaatt gcaaaatcca gattgtgttt gtaatgttta
     7861 atctgattgt attgagtggc gtttattatg aaaaataaat caggtaattc ttgttataga
     7921 gaagcagtcg atattcatag ctcacttgag ctttcattta tattgcataa atatcatttg
     7981 cgtgtagatg agaagaaatc tcgaaatgta ttcagcgcca tacgtgatga gctcatcctt
     8041 gatggggatt cacgacaaaa cttagctact ttctgtcaaa catggcttga tgatgaaatc
     8101 cgtgagttgc ttaatttagc tattgataaa aacatgattt ataaagatga atatccacaa
     8161 atggcggaac ctgaaggtcg ctgtgtgcgt atgctttccg attcgtggag tttccccgat
     8221 tcgcgacaca ccttaggttg ctcaaccatt ggttcatctg aagctgctat gctcggccgc
     8281 ttggcgttaa aatggcagtg gtgtaaaaaa cgcgaagtac aaggtaaatc aaccgaacct
     8341 gatttgcgat cctgtgcaaa tatgctggca taaattctgc cgttactgtg atgtgaaatt
     8401 gtgtgaaatt ccacttgatg atcatcacct gataatgaat gctaaagagg ttttgaagca
     8461 tgtcgatgaa aacactattg gtgtcgttac gactttaggt attacttatt tttgtaaagc
     8521 agattgcgtt agtgttttta agataagtgt taaagttaat attagttttt atatttacat
     8581 cgtaattata tgtttctatt ttaaaaatgt tcgagagtat aaaaatgtct aatgctaata
     8641 tcgcacccaa aagtaagaaa atgacagtgg ggacactcgc tatcatgaat ataactgctg
     8701 tagtaagtct acgatttata ccttctgaag cagaatacgg cttaggtgcc attttttact
     8761 atacctttgc tgcaattatg tttttagtac ctgttgcctt tgttgcggct gagctagcta
     8821 caacatatcc acaaaaaggg ggggcatttc gatggatagg agaagcattt ggggctcgct
     8881 ggggttttgt aggaatgctt atgacctggc tacaagtcat cccctacttt ccaacagtac
     8941 tgacatttgg tgcagtctcg gttgcattta ttgatgttga tatgggggtg gcagaatcaa
     9001 ttgccagtaa taaatggtat attttatttt tcgtactatt tgtttactgg ggagctattt
     9061 gtgttgcatt acgtggcgtt ggtattttct cgcaggtatc taagtggtgc ggtattatag
     9121 ggacaattat tccagctatt atccttgtta ttcttggttt ctcctatctg tggttcagcg
     9181 gtaaaccagc ggtgatccca ttaggttggg gtgatctttt ccctaatata gctaacttcc
     9241 agaatattgt tcttgccgcc agcatatttc ttgcttatgc agggatggaa atgaatgcgg
     9301 tccatgttaa cgatttagat aatccaacta aaaaataccc aattgcaatt accatcgctt
     9361 ctataggtac tattgcaata ttcctgctca gtactttagg ggtcgccttt attattccta
     9421 cagataaaat tagtctaact caaagtttgt tggttgccta cgatatgctg ttcgaatggg
     9481 ctggagtgcc atggcttggc tctgtgatgg catttatgct agcaattggc gtactgggtg
     9541 gggtggttac ttggattgca gggcctaata caggtgtatt agcgatcgcg aaagctggtt
     9601 atttacctaa gtttttccaa aaaaccaatc gtcatggaat ggggcaccat ctaatgtttg
     9661 tacaaggtat aattgtctca gttctttctg ttacttttgt gataatgcct tcagttcaag
     9721 cagcttttca gattttatca cagttaacag ttatgctcta tttagttatg tatatgctca
     9781 tgttcgctag tgctatctat ctacgacatt ctcaacctga tacaaaacgc ccatatcgag
     9841 cccctgcttt atttttctgg gcaacacttg gattcttagg gtcttttctg gcttttgtat
     9901 taagttttat tccgcccgag caaatcccaa ctggtagtcc aaccagctat gttctttatt
     9961 tgttagtatt agttgcccta tttttattaa ttccaatatt aatttacgcc ttccgaaaat
    10021 ctgactggaa agatccaaac tctgatttcg aaccattttc ttgggaaaaa agaactagta
    10081 attaaatttt ccataagcta caaaagatga ggttaaatat gtttagtgct gaatctccaa
    10141 ttggtgtaat agactcaggt gtcggtggat tgacatcggt aaaggaaata attaatttgc
    10201 ttcctggtga agatattatc tattgtggag ataatatgaa tgcaccttat ggcaaccgtt
    10261 ctgccgatga tatcatcagt ttgactaaaa aaatgctgac ttttttacag tcaaggaatg
    10321 tcaaactagt tgctgtcgca tgtaatacta tatcctcaac tttggaatcc gaagaataca
    10381 gcggatatgc taagagcttt ccgtttccta tcttatccat cattgaacct gcagtagaag
    10441 atgtgattcg ccagcaatat aaaaatgttg gtattatcgc taccgagttt actattaaaa
    10501 cggggtgtca caaagagcta ataaaaaaat tgaattcaac aataaatgta tttggagaac
    10561 caagcaaaaa tcttgcaatg ttaattgagg aaggtaatct taatgctcct gcaatcttga
    10621 atgacatcaa aaaacatgtt aaccatttaa tttccctcca tccagtaaat gaaattattc
    10681 tcggctgtac tcactatcca attgttcaga atttttttga agcagttgca ccggatataa
    10741 aatttattaa tcctgcacat gatcaggcta tctctattaa aaatcacttg ggacagctaa
    10801 atttattaaa ctcctcaaat ataggaactt tgcaaataaa tactagtgga agtatggaga
    10861 tctacaaaac ggtactaggg gaattgagca ttacgaaacc tcatacattc agtatcaggc
    10921 agttttaaat ttatccggta caccgtaaaa tcccgtcctt ctgaactgca ccccaaaagt
    10981 tggacaccaa ctgagtaagg tgcattttat ggacaaaccc agttacatac tgggtagtgc
    11041 atccaattag tagaacatgt gtttttcgat aaacgctccg atcacttttt cgtggatctc
    11101 aactgagcgt gagaagcaga ttgttttacg acccaaccgc ttaatgcggg tgcgtagcgt
    11161 cagattatta cgctcaatgc gttgggtgaa tattttgccg gtcagatgct tattcttcgg
    11221 cacctcccgg ccatagctgc cccagtcatc gctggtcagc atgccgatgt tgaagggtgt
    11281 aagcagtgcc agtagctccc ggcacgtttg atcggttcgg ggaccaaaag tgtaggccag
    11341 tacaccgcct gttttggtgt tgtacgcgta cccgagccaa tgttgccggg ctttactgcc
    11401 aacgtagctc cattgctcat caagctcgca gataaacgcc acatcagcat gggcaacagg
    11461 cgaagaagtt attcgctttg gcgcgagttt tttaaagtcc ggatgacggt gttactacca
    11521 attttcagtg tcctggcggt atcgcgaacc ccggcaccat taaaggccat ttcagttatc
    11581 agctctttca tacccggctt acgtgcttga taagtataag tgaactgaaa cacacggtgg
    11641 cagtcacggc agcgaagtct gtcacggcct ttagggttct gaccatggcg gtaaacctga
    11701 gctgactgac aacggggaca atgaatatta acgctggtca tgagataacc tcaaaagccc
    11761 gtattataca tcagattcaa ctaattagag gcatcaccca tccagcatta taaaacatac
    11821 taacaataac taaattataa taatcacaac ttacaatcaa ataataaatg cataagatta
    11881 acaatgattt tttcgggtta tatttatcta aaaaagcaga acattatgta acttattatt
    11941 ttctaagcga aaatttattg tgcagtcaga ctaacaaact tgcgtaccac ccgaagaaca
    12001 tgcgaagatt taagtattct ttcttttttg gtttttattt ttcattttct cttttcttct
    12061 ctttccggtt ctgttctggt tttggttttt tcctttttat ttttattctt tcttcagggt
    12121 tatgctaaaa caccaaaaca tttttcacca caaaagcatc caatcatctt gccagaccaa
    12181 tcaccttaac cggatacact gccggatata atcccactaa cacccagaca ccaagcacca
    12241 tacctgataa ctaccgtatt caggaaggaa atgcgagtga tttcaccgtc accggcacaa
    12301 ccgatccgcc aaaaagaggc gtaccaatgt cgatatagtc ccccgcgcgg acaatcactt
    12361 cgtcaatgac tgccaacggg agttgttgta gctgtgggcg caacaacatg cccagagctt
    12421 taccaaagtc ctgcccggcc accaccagca ggggatgcgg attcggaaaa cgcgcgacga
    12481 aatcgaccag cgcgttgatg accgtcagaa ccgcggcgta acgcacaggc agcgaggcgg
    12541 gaagcgccag cacgtacacg tcagttttgg gatcaagatc cagctgaagc agcgcctgtt
    12601 gccaggcact caccagatcc gtttcatcaa tcgggatcgc caccggcaaa ttgcgcagcg
    12661 gcagttgtac gccctccagc cagattgtgc tgccagagag cgaaagggta tgtgcacccg
    12721 cgccaatcac cgtggagcgt acggtttgcg ccggaaactg cacattcatc tcacgcaggc
    12781 gcggatggtc atgcagcgcc gttgccagaa gtgggccaat atcggcaaaa cagaacgggt
    12841 tggcgggctg gtggcgataa cattcgccca cgcctccaga aagcgtaatg atttcgggcg
    12901 taacacctgc gggcagcaaa ccggtttgca tcaatgcctg cgcgagcggc gaaagcgttc
    12961 cgtcaatcac ttcgacaatc agcgccgcca tccgccgggt aacctgcacc agctgcgcgc
    13021 cggtcagcga acgggcgtca gtgcctgcac cgaagcactc atccacaatc atctgccccg
    13081 gtttatgagc gtaaaccacg cgcccctggc tgtcggtttc cagcaggcga ccaccgacgt
    13141 tgagacaggc agtgccgctg atttttccgg catcgaacag ggcgtagttc gcggtgccac
    13201 cgccgatatc gatattcagt acccgacaca gccgttgttc agaaagggtt tgcgccccgg
    13261 ctccgtgacc ggcgatcacg gattcgaggt gcggcccggc gctggcgacg acaaaatcgc
    13321 ccagcgactg tttttccttc atcacttttt ccatcagctg tgcgaccagg ctttcggtaa
    13381 actggccttc cggcagctgg gcaatgatgg tttcgcggat gcgctggctt tcggttttgt
    13441 cggctggcac gctggcgggc acggacgccg ttaccgggat cgattcatca cattcggtga
    13501 tggtaaaacc cagtagatcc gccacttcac gggcttccgg ggtaataatg ctggcgcgca
    13561 gaaccacgga cattgcctgt tcgccgcgcg cgtgtgcttc acgaatatca ttcgctgtga
    13621 tgagtttttt cacctgcttc ctcctgtttg gtgagagatg ccagataatc caccagttgc
    13681 tgcacgcttt gcgggtcgtg gctgtttagc tcgaaaatcg gctcttcgaa acccgtctca
    13741 agcaataact ttcgcgttgc ggcgacgtca gcatctggca tgtccgtttt actgatgacg
    13801 gcgatttgcc gtttgctgac gccaatatcc agcaacccgg caggcaaccg actttccggg
    13861 tcatttgcgc catggacata gatcagcatg tctacatcct gtagcgtggt aattaaggcg
    13921 tgataccagc ggggatggct aaaatattca cccggcgtat caatatcgcc cttatcatta
    13981 aattccacgg ccagtgtttt tctggcgagg gtataattcc cctgcaacgc attaaataac
    14041 gttgtttttc ctgccccgac gctacccaca aacgcaattc gtttcatgaa tgcctcgtat
    14101 taacttttgg tcatttcaca aagcgtataa tttaataagc gacctaaacc gctgacggtt
    14161 tgtgataacg cttcttctac tgcgcccacc gagccataaa tcaccaatgc accgctgaag
    14221 cggtcgagaa aaccgatatg cacatcagcg gctttcagcg ctaaatcacc ggcgatcatc
    14281 gccgtttcgc cgggagttag cgtcataatg ccaattgcgc ccgcatccgg aacgccgatt
    14341 ttcttcgcca gttcctcacc gggatgcgcg atgagatgcg ccagtgtgac ctgtttgccg
    14401 ggcacaaatt cctgaatgat gcgttcttta tccatgagtc gccgcctgtg aaaaactttc
    14461 gtgtatcgtg acaatttttc gtcagagtaa ataacaaaat tcggcaagcg cgtttaaaag
    14521 gtgaggtaga tcaataagaa tggagagata tttactgtta tgaataaact ttcatatgcg
    14581 acgcatgaat ataaataaaa taatatcgag atattttata tactgatttt tttattattc
    14641 gcaaactcag taataagctt tgctaatata tttcaggaaa accgcttacc ctgaatattc
    14701 agggtaagcg tgagagttaa gaatgaatta cagcggttgg gtttgcgctt caaccacggc
    14761 cagcgccacc atattgacga tacgacgcac cgatgcgatc ggcgttaaca cgtgaaccgg
    14821 tttcgccacg cccatcagca ccggaccgac agtcacacct tccgagctgg aaacgcgcag
    14881 taagttgtaa ctaatgcggg cagcttccat gttcggcatc accagaatat tggcagaacc
    14941 tttcaaaggg ctgtccggca tacggtcgtt gcgaatcgct tccaccagcg ctgcatcgcc
    15001 gtgcatttca ccatcaatca tcagatctgg cgcacgatcc ctgaccagtt ccagcgcctg
    15061 acgcattttg ctcgacgacg ggcagtcaga agaaccaaag ttggagtgcg acaacagagc
    15121 aacgcgcggc tcaataccaa aacgacggac agtttctgcc gccatcaagg tgatctccgc
    15181 cagctcttct gcgtctggtt cgtcattgac gtaggtatcg gcaataaagg tgttaccact
    15241 cggcagcagc agcgcattca ttgcacctgc ggtgtgaacg ccatcgcgat aaccaaagac
    15301 atttttcacc acactaaaat gttcatgata atcgcccacc gtaccgcaga tcattgcatc
    15361 ggcttcccca cgctgaacca tgatcgcgcc gatcactgtc gggttactga tcagcgcacg
    15421 ctgcgcctgt tcctgagtga caccgcgacg cttcatgatc tggaagtatt cggtccagta
    15481 ctctttaaag cgcggatcgg attcgttatt gacgatttca aaatcaacgc ccgctttgat
    15541 ctgcaagccc agtttctgaa tgcgcatttc gatcacgttc ggacgaccga taaggatcgg
    15601 tttcgccagt cccagcgtta ccagttcctg agtcgcatgc agaacgcgcg cctcttcccc
    15661 ttccggcaga actacgcgct tcggcgcttt gcgagcctgg gagaaaatcg gcttcataaa
    15721 caggttggtt ttgtaaacga actcagtcag cttgtcgatg tagacgtcga aatcagcaat
    15781 cggacgcgtc gccacgcccg actccatcgc cgcttttgcg accgcaggag cgatcttaac
    15841 gatcaagcgc ggatcaaacg gttttggaat gatgtattcc ggaccaaagc tcagatcctg
    15901 aacgccatac gctgaagcca ccacttcact ctgttctgca tgggccagtt ctgcaatcgc
    15961 gcgtaccgcc gccagtttca tctcttcgtt gatggcggtt gcgccaacgt ccagcgcgcc
    16021 acggaagatg aacgggaagc aaagaacgtt gttcacctgg ttcgggtagt cggaacggcc
    16081 ggtacagatg atggcatccg gacgcacttc tttcgccagc ggcggcagaa tttccggttc
    16141 cgggttcgcc agcgccagga tcattggtgc ccgagccatt ttcttcacca tttcctgggt
    16201 cagcactttc gggccggaac agcccaggaa aatatccgca ccttcaatca catcatcgag
    16261 ggtacgtttg ccgtcatcca ccaccgcata tgcggctttg gtttccgcca tatttggctc
    16321 acggccctga tagataacgc cttttgaatc gcaaaccacg atgttatgtt tttgcagccc
    16381 cagcgctacc agcaggttca tacaggcgat tgctgcggca cccgcgccgg aaaccaccat
    16441 ccgcacgtcg gagatgtttt tctccaccac gcgcaagccg ttgaggatgg cggcagtgct
    16501 gatgattgcc gtgccgtgct gatcgtcgtg gaataccgga atattcatcc gctcgcgcag
    16561 tttctgttca atatagaaac attctggcgc tttaatgtct tcgaggttga tgccgccgaa
    16621 ggtcggttcg agcgcggcga cgacttcaat aaatttgtcc gggtcgagtt catcaacttc
    16681 aatgtcaaat acatcaatcc cagcgaattt cttaaataga acgcccttgc cttccatcac
    16741 cggtttgcct gccagcgcgc caatgttgcc taaccccagc accgccgtac cgttagagat
    16801 caccgcaacc aggttaccgc gcgcggtata tttgtaggct tttagcgggt ctttttcgat
    16861 ttcaagacaa ggtgcggcaa cgcctggtga gtaggccagc gccagatcgc gctgcgttgc
    16921 cagaggcttg gttggagaaa cctggatttt ccctggaact ggaaattcat ggaaatcaag
    16981 tgcactttgt tttaactggt catccatttg gttgttcctt tcacgtaacg ttcacaaata
    17041 aagtgtgtgg gcaacagccc ctgcccacaa cgtggcgcac attattaccc tgccggagtc
    17101 tacagacttt gagcaagtcc aaactctcac cattaatata atgttttggt aataatccta
    17161 taacactgat gttacctgct taatccagca ataccatgcc tgtctgctat gcttttttga
    17221 tgcgtttagc gaaatttctc agaagtgtga attaacgcac tcatctaaca ctttactttt
    17281 caaggagtat ttcctatgaa cgagttagac ggcatcaaac agttcaccac tgtcgtggca
    17341 gacagctgcg atattgagtc cattcgccat tatcatcccc aggatgccac cactaatcct
    17401 tcgctgttac tcaaggctgc cggattatca caatatgagc atttaataga cgatgctatc
    17461 gcctggggta aaaaaaatgg caagacccag gaacaacagg tggtcgcagc gtgtgacaaa
    17521 ctggcggtca atttcggtgc tgaaattctc aaaatcgtac ccggttgcgt gtcaacagaa
    17581 gttgatgcac gcctctcttt tgataaagaa aagagtattg agaaggcgcg ccatctggtg
    17641 gacttgtatc agcaacaagg cgttgagaaa tcacgcattt tgatcaaact ggcttcgacc
    17701 tgggaaggaa ttcgcgcggc cgaagagctg gaaaaagaag gtattaactg caacctgacg
    17761 ctgctgtttt cttttaccca ggcgcgggcc tgtgcggaag caggcgtttt tctgatttcg
    17821 ccgtttgtag ggcgtattta tgactggtat caggcacgca agccgatgga cccgtatgtg
    17881 gtggaagaag atccgggcgt taaatcggtg cgcaatatct acgactacta taagcaacac
    17941 cactatgaaa ccattgtgat gggcgcgagc ttccgtcgca ccgaacaaat cctcgcctta
    18001 accggctgcg atcgactgac tatcgcaccg aatttgctga aggagctgca ggaaaaagtt
    18061 tctccagtgg tacgtaaatt aatcccgcct tctcagacgt tcccacgccc agcacccatg
    18121 agcgaagcgg agttccgttg ggagcacaat caggatgcga tggcggtaga aaaactgtct
    18181 gaaggcattc gtctgttcgc tgttgatcaa cgcaaactgg aagatcttct tgccgccaaa
    18241 ctataaacca gccacggagt gttatatgtc ccgaaaagac cttgccaatg cgattcgcgc
    18301 actcagtatg gatgctgtac aaaaagccaa ttctggtcat cccggcgcgc cgatgggcat
    18361 ggctgatatt gccgaagtgc tgtggaacga ttttcttaaa cataatccta ccgacccaac
    18421 ctggtatgat cgcgaccgct ttattctttc caacggtcac gcgtcgatgc tgctctacag
    18481 tttgctacat ctgaccggtt acgacctgcc gctggaagaa ctgaagaact tccgtcagtt
    18541 gcattcgaaa accccaggcc acccggagat tggctatacg ccaggcgttg aaaccaccac
    18601 cggcccgctt ggacaaggtt tggcgaacgc cgtcgggctg gcgatagcag agcgtacact
    18661 ggcggcgcag tttaaccagc cagaccatga gatcgtcgat cacttcacct atgtgtttat
    18721 gggcgacggc tgcctgatgg aaggtatttc ccacgaagtc tgttcgctgg caggcacgct
    18781 gggactaggc aagctgattg gtttttacga tcacaacggt atttccatcg acggtgaaac
    18841 agaaggctgg tttaccgacg atacggcaaa acgttttgaa gcctatcact ggcatgtgat
    18901 ccatgaaatc gacggtcacg atccgcaggc ggtgaaggaa gcgatccttg aagcgcaaag
    18961 cgtgaaagat aagccgtcgc tgattatctg ccgtacggtg attggctttg gttcgccgaa
    19021 taaagcaggt aaggaagagg cgcacggcgc accactgggg gaagaagaag tggcgctggc
    19081 acggcaaaaa ctgggctggc accatccgcc atttgagatc cctaaagaga tttatcacgc
    19141 ctgggatgcc cgtgaaaaag gcgaaaaagc gcagcagagc tggaatgaga agtttgccgc
    19201 ctataaaaag gctcatccgc aactggcaga agagtttccc cgacggatga gcggtggttt
    19261 accgaaggac tgggagaaaa cgactcagaa atatatcaat gagttacagg caaatccggc
    19321 gaaaatcgct acccgtaagg cttcgcaaaa tacgcttaac gcttacgggc cgatgctgcc
    19381 tgagttgctc ggcggttcgg cggatctggc tcccagcaac ctgaccatct ggaaaggttc
    19441 tgtttcgctg aaggaagatc cggcgggcaa ctacattcac tacggggtgc gtgaatttgg
    19501 catgaccgct atcgccaacg gcatcgcgca ccacggcggc tttgtgccgt ataccgcgac
    19561 gttcctgatg tttgttgaat acgcccgtaa cgccgcgcgg atggcggcac tgatgaaagc
    19621 gcggcagatt atggtttata cccacgactc aattggcctg ggcgaagatg gtccgacgca
    19681 ccaggctgtt gagcaactgg ccagcctgcg cttaacgcca aatttcagca cctggcgacc
    19741 gtgcgatcag atggaagcgg cggtgggctg gaagctggcg gttgagcgcc acaacggacc
    19801 gacggcactg atcctctcaa ggcagaatct ggcccaggtg gaacgtacgc cggatcaggt
    19861 taaagagatt gctcgtggcg gttatgtgct gaaagacagc ggcggtaagc cagatattat
    19921 tctgattgcc accggttcag agatggaaat taccctgcaa gcggcagaga aattagcagg
    19981 agaaggtcgc aatgtacgcg tagtttccct gccctcgacc gatattttcg acgcccagga
    20041 tgaggaatat cgggagtcgg tgttgccttc taacgttgcg gctcgcgtgg cggtggaagc
    20101 aggtattgcc gattactggt acaagtatgt tggtctgaaa ggggcaattg tcgggatgac
    20161 gggttacggg gaatctgctc cggcggataa gctgttcccg ttctttggct ttaccgccga
    20221 gaatattgtg gcaaaagcgc ataaggtgct gggagtgaaa ggtgcctgat ggtgatcgcc
    20281 ggatgctgta tgttcccctc accctaaccc tctccccaga agggcgaggg gacggtccgt
    20341 gcgcttttta gggccgcatc tgcgattttg tttaacgggt gatccacaac gtgggcctgg
    20401 cgtctggccc atgccagtta tcgcaggtag gttctgcggc gtaacgcacc aggcgaaaac
    20461 gctgaccgtc aaagcgccag cgcgcctgaa tgccacaatc gcttaatccg cgccctttcg
    20521 ctaaggtcac cagttcacgc gatttctcat caaatgttgc gttcatcagt tccagttcat
    20581 tcgtctcctg gccgttgttg aacggcaaac gcaaccgaac cgggcgcgag gctagcggct
    20641 ttttacgcga cacaatccat gccaaatcaa tggtgttata ggcccctgcc tcacagctaa
    20701 tcatcatcag tgctttatca tcggtcagcg cagtgacatt cacctcacga cgtaacggat
    20761 caagcgagca gcgcagacca ttcatccgcc agtttccata atccagcaaa tcgttacgtt
    20821 cttcgagtga gagtggcgtc ggcgttgggt taaccaccgc gacctctttc agcgcaggcg
    20881 cgggcggtac gctgagaggc ggttcgtccc ctttcttgat ccacgcggtt tcactgccaa
    20941 cgcgcttttg ctgagcatca ataaacaaca acgccgcttt taagccgctc agagaaatgg
    21001 tctgattgcc gtcgcgtaag gtgatcgcct tcccttcctg aattatctgc aaaaacgcgg
    21061 tgatggttgc cgtatcatcg gttactaata accatggtga aatccgccac ttgtcaccac
    21121 ttaatgccaa cggctcacca tctaatagca gccgtggcgc tatctcccct tcagacgcat
    21181 ccggcgactt caacccaccg cgctcaatac gtaaaacggc atcggtatgc gccccagcgc
    21241 tgcggctcag ggtcatcacc agtccattat gatcgcccgt attgcgcgcc acacaaaaat
    21301 tttgattatt acaggtgacc tgccagtcgg aaaacgcccg ttgcgctggt gccgcccaca
    21361 ccaaagacgt cggcaacaaa gcgaaaaaaa agagaaggaa aatgcgatag cgcatggacg
    21421 gtacgacccc agaagttcaa acaaacagca caagtcgtat cttcgttgcg caggtagcgc
    21481 ggctcaatcg gatttgtcgg attgagccaa taataaacaa cgaaatatta ttgctatttt
    21541 tcagtccatt aaatgtgaca tttg
//
LOCUS       DAJTSI010000062        21512 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000062 DAJTSI010000000
VERSION     DAJTSI010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.062"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="OO327_002096"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="OO327_002096"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR6018637.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="OO327_002097"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="OO327_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR6018638.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /locus_tag="OO327_002098"
     CDS             complement(2649..3200)
                     /locus_tag="OO327_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR6018639.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="OO327_002099"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="OO327_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR6018640.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5211
                     /gene="cybC"
                     /locus_tag="OO327_002100"
                     /pseudo
     CDS             4829..5211
                     /gene="cybC"
                     /locus_tag="OO327_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="OO327_002101"
     CDS             complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="OO327_002101"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR6018641.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="OO327_002102"
     CDS             complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="OO327_002102"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR6018642.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8410..10065)
                     /gene="treC"
                     /locus_tag="OO327_002103"
     CDS             complement(8410..10065)
                     /gene="treC"
                     /locus_tag="OO327_002103"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR6018643.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10115..11536)
                     /gene="treB"
                     /locus_tag="OO327_002104"
     CDS             complement(10115..11536)
                     /gene="treB"
                     /locus_tag="OO327_002104"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCR6018644.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11655..12602)
                     /gene="treR"
                     /locus_tag="OO327_002105"
     CDS             complement(11655..12602)
                     /gene="treR"
                     /locus_tag="OO327_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR6018645.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12787..12840
                     /gene="mgtL"
                     /locus_tag="OO327_002106"
     CDS             12787..12840
                     /gene="mgtL"
                     /locus_tag="OO327_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR6018646.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12981..15677
                     /gene="mgtA"
                     /locus_tag="OO327_002107"
     CDS             12981..15677
                     /gene="mgtA"
                     /locus_tag="OO327_002107"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR6018647.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="OO327_002108"
     CDS             complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="OO327_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR6018648.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="OO327_002109"
     CDS             complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="OO327_002109"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR6018649.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="OO327_002110"
     CDS             complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="OO327_002110"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR6018650.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="OO327_002111"
     CDS             complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="OO327_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR6018651.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18170..18565)
                     /locus_tag="OO327_002112"
     CDS             complement(18170..18565)
                     /locus_tag="OO327_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6018652.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="OO327_002113"
     CDS             complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="OO327_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6018653.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19481..20074
                     /locus_tag="OO327_002114"
     CDS             19481..20074
                     /locus_tag="OO327_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR6018654.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20219..20671
                     /gene="tabA"
                     /locus_tag="OO327_002115"
     CDS             20219..20671
                     /gene="tabA"
                     /locus_tag="OO327_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR6018655.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20794..>21512
                     /locus_tag="OO327_002116"
     CDS             20794..>21512
                     /locus_tag="OO327_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6018656.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNN
                     SQVSCKGSVDSTITDLLEKAL"
BASE COUNT         5196 a         5324 c         5668 g         5324 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgctg gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgctgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggcgcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaaaagaagc gcaggctgct
     5161 gcagagcaac tgaaaacgac ccgcaacgcc tatcaccaga agtatcgtta attcctcatt
     5221 tccctgttgc ctgcactcag gtaacaggga acttatcatc gcaaatgatg caccacctga
     5281 ttgctgctgc cgcgccagat cagggaaggg tcttttaaat cctgcacaaa tttgccgtcg
     5341 accagcacgt taatcaaatc aacaacctgc atttgcgcag cgttgagttc gtcgagttta
     5401 tagcctgtcc acacccagat gtctttaccc ggacactcgg cgcggatgcg ttttaccagt
     5461 ttcagaatat ccggcacgtt ttgcggatgc agcggatcgc cgccggagag ggaaatcccc
     5521 tggcgtttga tacgagtgtc attcagatct ttaatgatct tgtcttccat tgctttggta
     5581 aatggctgac cagaatttac ccgccatgtg cttttgttat agcaaccggg gcattcatga
     5641 acacacccgg agacaaacag ggtgcagcga gtgccggggc cgttgacgat gtcgacagga
     5701 tagtactgat gataattcat tatttcgcct ggcttatcag tccgcgtgta ggctgcatcc
     5761 ggtaatcaat gcctgatgcg acgctgtcgc gtcttatcag gcctacagcc gttgccaaat
     5821 gtaggccgga taaggcgttt acgccgcatc cggcatttgc ttagcgcaga gtgaagatta
     5881 acctatctgc ccattcccca aatgtttaac gcggcgctta acttcttcct gcttaccagc
     5941 gttaaacgga cgtgcatccg ggctacctaa atatccgcac acgcggcgag ttaccgacac
     6001 acgggaggcg tcatggttac cacatttcgg gcaagtgaag cctttgctgg tgcactcgaa
     6061 ctcaccggta aagccacact cgtagcactc atcaatcggt gtattggtgc cgtaatacgg
     6121 tacatgctga tagctgtaat cccagacatc ttccagcgct ttcaggttgt gctgaatgtt
     6181 tggatactcg ccgtagcaaa tgaaaccacc gttcgccagc ggcgggtaag gcgcttcaaa
     6241 gtcgatcttg tcgtacgggt tcaccttctt ctccacatca aggtggaaac tgttggtgta
     6301 gtaaccttta tcggtcacgc ccggcaccac gccaaactca gcagtatcga gacggcagaa
     6361 gcggtcgcac aggttttcac tcggcgtgct gtaaagactg aaaccataac ccgtttcttc
     6421 tttccactga tccactgcct gacgcagacg ttcaacaatg gcgatacctt tcgcgcgaag
     6481 cttctcgttg tcgtaaacat gctcgccgct gaacagcgcg ttaatggttt cgtggatgcc
     6541 gatgtaaccc agcgaaatag acgcacgacc gtttttgaag atttcagaaa catcatcgtc
     6601 agcattcaga cgtacgccac aagcaccttc catatagagg atcggggcga cgcgcgcttt
     6661 cacgccttcg agacgagcga tacgggtcat cagtgcctta cgtgccagca ccagacgttc
     6721 atccagcagc ttccagaagg tggcttcatc gccttttgct tccagagcaa tacgcggcag
     6781 gttcaggctg atcacgccga ggttgttacg accatcgtgg atctgctcgc cgttttcatt
     6841 ttcccacacg ccgaggaagc tgcggcagcc catcggggtt ttaaacgaac cggtgacgtt
     6901 cactacctga tcgtagttca ggatatccgg atacatgcgc ttgcttgcgc actccagcgc
     6961 cagctgtttg atgtcgtagt tcggatcgcc ttttttatgg ttcaggccat cgcgaatcgc
     7021 aaacaccagt ttcgggaaca ccgcagtttt acggttttta ccgaggcctg cgatacggtt
     7081 acgcaggatc gattcctgaa tcaggcgcga ttcccagctg gtgcccagac caaaaccaaa
     7141 ggttacaaac ggcgtctgac cgttggcggt gtgcagggtg tttacttcgt actccagcga
     7201 ctggaaggca tcgtagcact ctttgatggt acgagagtta gcgtagcctt cggcgtccgg
     7261 gatgttccac tcttctgcgg tcttgcgatg tttgttgtag ctggcggtaa caaacggcgc
     7321 cagcacttca tcgatacggt taatggtggt gccgccataa atatggctgg cgacctgagc
     7381 aataatctgc gcagttaccg cggttgccgt agagatcgac ttcggcggtt caatctcggc
     7441 gttccccatt ttaaaaccct gggtcagcat gcctttcagg tcgatcaaca tgcagttgaa
     7501 catcgggaag aacggcgagt aatcgagatc gtgatagtga atatcgccac gctcatgtgc
     7561 ctgcaccacg tcacgcggca gcaggtgctg gcgtgcatag tgtttagcca cgatcccggc
     7621 cagcaggtcg cgctgggttg gaatcacctt gctgtctttg ttggcgtttt cgttgagtaa
     7681 cgaggcgttg gtctgctcga ccagaccacg gatctcctgg ttcaggcgac cgcgtttttc
     7741 acgttcaatg tcgcgatcgt gacggtactc gatgtaagca cgagccagtt gtttgtatgg
     7801 acccgacatc agctggtttt caactgcggt ctggatctcg ttgatatcca cctggttgcg
     7861 cccctgcatc tgctcgttga caaccgcggc aacagtggcg caataatcgg catcatcgac
     7921 ttccgctgct ttagctgcac gcagaatcgc ttctttgatg cgctctgatt taaacggcac
     7981 tttgcagccg tctcgtttca tcacatgcgg tgtcatgatc gctccatatt tttaagaaca
     8041 ggttatccac agaaattggg aaaggcgttt tccggttttt tcgtttcgtt ttccggtgct
     8101 ttccgcaatc gccatccgct tatccacaac gccgcacctt tttcgtgggg agctgtagta
     8161 gcaattatag tcgattaata caacatattg ggttgggacg cattttaaag tctatatata
     8221 gtgctttgca tcaaggatgt tttaactttt tttgatgtag ctcaaagtaa aaagcagagc
     8281 gtacggatga cgggcgctac agcgttatgt aaatttttta atgaatttgc tggttgaaaa
     8341 atcaacaaaa acaacatgct gacagacaaa accccggaat gaccggcatt atcggggctt
     8401 agggaagatt tacttctgta accaccagac agcctcaaaa ggccgtaaat tcatggcaca
     8461 gggttgtggt gaggcttctt cgtagttatg catcacaagc tgccagttgc cgcgcatttg
     8521 ccctggctgc cagggttgga tctcacggct aaggttggca atgaccagca aggtttgccc
     8581 cttccattca cggcgatagc accacaatac agggctgttt ggcagcagat cctggtaatt
     8641 gccccatgtc aggatggctt cttgcttacg cagtgcgatt aacttttggt aggtgtaaaa
     8701 cacagaggag tcatcggcca gcgcggcttc tacgttgatt tgttgatagt tatcgcctag
     8761 gccaatccac ggttcgccag ccgtaaaccc ggcattatcg ccgttgctcc attgcatggg
     8821 cgtgcggctg ttgtcacggg atttactggc gaggattgcc aataactcgt cggcatcacg
     8881 accatcgttg cgcagctcgg caaacatatt gaggctctcc acgtcgcgat agtcagtaat
     8941 gcgcgtgaaa tgcgggttgg tcatgccaat ctcttcgccc tggtagatat acggcgttcc
     9001 ctgcatgcca tgcagcacca tcgccagcat ttttgccgca ggcacgcggt attcaccttc
     9061 atcaccaaag cgagaaacaa tgcgcggctg atcgtggtta caccagaaca aggcattcca
     9121 tgccacgttg tgcattcctt gttgccagtg gcggaacaat gttttcaacg ccacaaagtc
     9181 aggtttagcc agcgtccatt tttcaccacc gggataatcg accttcaggt gatgaaaatt
     9241 aaaggtcatc gacaattcac tgcctgtcag tgccgcgtat cgctggcaat gctcaaggct
     9301 ggtggaggac atttcaccta cggtcattaa cccgcgtggc gtaaacacat cgcggttcat
     9361 ctcgtgcaaa aactcgtgtg ctcgtggccc gtcggtgtag aagcgacgcc cgtcgccatc
     9421 caggtcttca gggaaacgcg ggtctttgga gatcagattc accacatcca ggcgcaaccc
     9481 gtcgacccca cgatcggccc agaattcaca gacttttttc agctctgcgc gtaccgctgg
     9541 attctcccag ttgagatccg cctgttctgg tgcaaagaga tgcaaatagt actgttcgct
     9601 ttccgcatgc cagcgccacg cactaccgcc aaattttgaa cgccagttgt tcggtggcgt
     9661 ttctggttcg ccatcgcgcc agatataaaa ctggcggtaa gggctttctt tgttcagcgc
     9721 ctcgcgaaac caggcatgtt gggtagaggt atggttaaac accatatcga gaatgatacg
     9781 aatcccgcgc gattttgcct gcgtcaccag ttcgtcaaaa tcgtccagcg tgccgtaggt
     9841 gggatcaatc gccgtatagt tcgctacatc gtaaccgtta tcgacctggg gagagacata
     9901 aaagggggtt agccagatgg catcaacgcc cagtttatgc agatagtcca ggcgttggat
     9961 aacgccacgt aaatcgccgg tgccgctacc cgtggtgtcc tgaaaactct ttggataaat
    10021 ctggtagata acgccgtttt gccaccagtg gggaagatta gtcattacgt tattcctgca
    10081 aatgcgaggg ggcgcaattg cgccccgaag aaaattaaac aatgtccagc gtgcccaggc
    10141 ggtatttccg ctgatagata aacgaggtga gtacaatcgg gatgatgatg gcgatagcca
    10201 ttgccagcgc aaatacctgc cagtagctcg gttgaataga gagaattccc ggcaggcccc
    10261 ctacgccgat accattcgcc ataacgccgt tcaggccgca tagcaatcct gccagaccag
    10321 aaccaatcat cgcgcacagc atcgggaagc gatatttcag gttgatgccg tacattgcag
    10381 gctcagtgac cccaagccag gcggagatag cggcaggcac ggagatctcg cgttcattgt
    10441 gcttgcggct ggaaatgata atgcctatca cggcggagcc ctgagcgata ttcgacagcg
    10501 caatcagcgg ccacactggc gtgccaccca tgctttgaat catctgcaaa tcaatagcaa
    10561 gggtggtctg gtgtacgccg gtgatcacca gcggcgcgta caggaagcca aacaatgccg
    10621 caccaatcgg agcaaagctg ccggtcatca ggtgacgtac cgcaaaggca acgccatcgc
    10681 caatcatgcg accaaacgga ccaatcagcg catgggcgag gaacaccgcg aggatcagcg
    10741 aacagacggg caccaccacc agatagaggt aatccggtac gatgcgttta aggcgagttt
    10801 caataacgcc cagcgccagc ccggctaaca gtgccgggat cacctgcgcc tgatagccca
    10861 ctttggcgat gctgaacatg ccaaagtccc acacttccgg cagctgctgc ccgagcagat
    10921 aagcgttcat cagctggggg gaaaccagtg tcacgccaag cacgatacca aggatcggcg
    10981 tgccgcccat ttttttcacc gctgaccagc aaatcccgac cggcaggtag aagaaaatcg
    11041 cttcaccgat caaccacaga aaatcgtaga tcgtttgcag ggaagggtac atttgcgcca
    11101 gcgtctgacc gttgctcatg ggcaaatcgc cgatcacatt gcgaaaaccg aggatcaaac
    11161 cgccgctaat caacgcgggc agcaacggga agaagatctc cgcgaaatga gagatcaact
    11221 gctcatgcca tttcatattt tgccgggcgg ctttttttac ctgctcttta tcaacctgcg
    11281 cctgtccggt tgacgctatt agtgcttgat agtaatcacc cacgttggtg ccaatcacca
    11341 cctgaaattg cccggcattg gtgaaacagc ctttcaccat ggggagttgc tcaatttctt
    11401 tcggtctggc attggccggt tggttgagga caaagcgcag gcgagtaata cagtggctca
    11461 ccgtcgcaat attgccgcgc ccgccgacca gttcaatcaa ccgatcgata tccgtttggt
    11521 ttattttgct catcataaag ccccatggca gatgacattt ttggttggct gcagaatata
    11581 ttgcgcggaa aaatttaaaa acgggaacgt tcccgaaacg cagcgaagat cacaatttat
    11641 cgttcaggaa acgatcagga cagggtggcg gggatgatga tttgttgcgg ttcgctgcgc
    11701 ccggttacct gtgcgatcaa ttggcaggcc gcctggcgtc cagcttcggc gtagccagga
    11761 tctacggtga cgatctccgg atgaaggaat ttcattaacg gcgtattacc tacgctcgcc
    11821 agttgcaagg tgtcgatgcg ttgctcttgc aggtatttac ttgcgccaag agccagcgtg
    11881 tcggttgcgc acagtaacgc ggtagtttca ggcgtaatca cttttgctac gttttcatag
    11941 ccttgcttca tagcaagccc tggcagagcg gcaacgggat gcagtttatg cgctttgcag
    12001 aacgccaggt aggcttcgtg acgtcgcttc ccggtcgtca cgtcactatg cggcacgccg
    12061 agataactga tattgcgatg cccctggtca tagagccgtt gcatcagaat cttgattgcc
    12121 ccttcgtcgt cataacagac cgaagcaaag ccttttgcgt cacgcgccag cagaaccagc
    12181 gatgactgcc agtgggctaa catttcttcg gttatgccgg taaaaccgaa cagcactacg
    12241 ccgtcgatat tacgccgttt cagcaccccc aaatgttcgg caactaattg tggggaaaac
    12301 tgattttcca tcatgattgg atcgtaacct tgttcataga acgctggcag catggtttga
    12361 acggcgagat tttctgacaa cgaatccaga cgggtaacaa tgatggcgac cactttatcg
    12421 ctttgcccac gcatagcgcg cgcagagcgg gaaggggaaa atccatgctg attcatcact
    12481 gcttcaacac gctcgcgcgt gcgctgactc acgccgcttt cgttattcag cacccgggaa
    12541 accgtagatt tccccacgcc gcttaagcgc gcgatgtctt tgatggtcag ccgattttgc
    12601 atcctgttgt cccataacgt gttgtttaat tatttgagcc taactttacc cgtgcattca
    12661 gcaatgggta aagtctggtt tatcgttggt ttagttgcca gcaggtatta tatcgccata
    12721 aatgctacga atattattgg attctcctta ttatttgcgg cgcttttttc acttaccgga
    12781 ggttatatgg aacctgatcc cacgcctctc cctcgacgga gattaaaact tttccggtaa
    12841 gcccgtcttt tcacggcgtt accggatgcg taaggccgtg acgttttaat gtccctgctc
    12901 agctttatta ccttcaggta aggcttcgcc acgcctgaag acatttctgt actgtttcag
    12961 acagtgcgga gggactcctt atgtttaaag aaatttttac ccggttcatt cgccatttac
    13021 cttcccgtct ggttcatcgc gatccattgc ctggcgcgca gcagacagtg aatacggcgg
    13081 tcccgccgtc cttaagtgcg cattgcctga aaatggcggt gatgcccgaa gaagaattgt
    13141 ggaaaacgtt cgacacccat ccggaaggat taaatcaggc ggaagtggaa tctgcccgcg
    13201 aacaacatgg tgataataaa ttacccgccc aacaaccgtc gccgtggtgg gtacatttat
    13261 gggtctgcta tcgcaacccc tttaatattt tgctcaccat tcttggtgct atttcttacg
    13321 ccacggaaga tttatttgcc gcaggtgtta tcgcgctaat ggtcgctatt tctacgttgc
    13381 tgaactttat tcaggaagct cgttccacca aagcggcaga tgccctgaaa gcgatggtca
    13441 gtaataccgc gacggtactg cgagtaatta acgacaaagg cgaaaatggc tggctggaga
    13501 tcccgataga ccagctggtg cccggcgata ttataaaact ggcggcgggg gatatgatcc
    13561 cggcagattt acgtatcttg caggcgcggg atctgttcgt cgctcaggcg tcgttaaccg
    13621 gtgagtctct gcccgtggaa aaagccgcta ctactcgcca gccggagcac agcaatccgc
    13681 tggagtgcga caccctgtgt tttatgggca ccaccgtggt gagcggcacg gcacaagcaa
    13741 tggtgattgc tacaggagcc aacacctggt ttggtcaact ggcggggcgt gttagtgagc
    13801 aggaaagcga gccgaatgcc tttcagcaag ggatcagccg cgttagtatg ctgctgattc
    13861 gctttatgct ggtgatggct ccggtggtgt tgcttatcaa cggttacacc aaaggcgact
    13921 ggtgggaagc ggcactgttt gcgctttcgg tcgcggtagg cttaacgccg gaaatgttgc
    13981 cgatgattgt tacctcgacg ctggcgcgcg gcgcagtaaa gctgtcgaaa cagaaagtga
    14041 tcgtcaaaca tctggatgcc attcagaact ttggcgcaat ggatattctg tgcactgata
    14101 aaaccggcac cctgacgcag gacaaaattg tgctggagaa tcataccgat atctccggta
    14161 aaaccagcga acgcgtgctg catagcgcgt ggttgaacag tcattaccag accggactta
    14221 aaaatctgct tgataccgcg gtgctcgaag gtacggatga agagtcagcg cgttcgctgg
    14281 ccagccgttg gcaaaaaatt gatgagattc cgtttgattt cgagcgtcgt cggatgtcgg
    14341 tggtagtggc agaaaatacc gaacaccatc agctggtttg caaaggtgca ttgcaggaaa
    14401 tcctcaatgt gtgttcgcag gtgcgtcaca atggcgagat tgtaccgctc gatgacacca
    14461 tgctgcgtaa gattaagcgg gttactgata cgctgaatcg tcaggggctg cgcgtggttg
    14521 cggtggcgac gaaatacctg ccagcgcgtg aaggagatta ccagcgggcg gatgaatccg
    14581 acctgatcct cgaaggatat attgctttcc ttgatccgcc aaaagagaca accgctccgg
    14641 cactgaaggc attaaaagcg agtggaatta ccgtaaaaat ccttactggc gacagtgagt
    14701 tagttgcagc gaaagtgtgc catgaagtgg ggctggatgc gggagaggtg gtcattggta
    14761 gtgatattga aacgctatct gacgatgaac tggcaaatct cgcacagcgc accacgctgt
    14821 ttgcccgcct gacgccgatg cataaagaac gcattgtgac tttactgaag cgcgaagggc
    14881 atgtggttgg ctttatgggc gatggtatta atgatgcgcc cgccttacgc gctgcggata
    14941 tcggcatttc tgtggacggc gcggtagata ttgcccgtga agcggctgat atcatcctgc
    15001 tggaaaaaag cctgatggtg ctggaagagg gggttattga gggacgtcgc actttcgcca
    15061 acatgctgaa atacatcaaa ttgacggcga gttctaactt cggtaatgtg ttcagcgtgc
    15121 tggtggcgag tgctttcttg cccttcctgc cgatgttgcc gttacacttg cttattcaga
    15181 acctgctgta cgatgtgtca caggtggcga tcccgtttga taacgtcgac gacgagcaaa
    15241 ttcaaaagcc gcagcgttgg aatccggcgg atctggggcg ctttatggtc ttcttcggac
    15301 cgatcagctc gattttcgat attctgacgt tttgcttgat gtggtgggta ttccatgcca
    15361 acacgccgga aacgcaaacg ctgttccagt cgggctggtt tgtggtggga ttactgtcgc
    15421 aaacgctgat tgtgcatatg atccgtaccc gccgtgtgcc gtttattcag agctgtgcat
    15481 cgtggccgtt aatgatcatg accgtgatcg tgatgattgt cgggatcgca ttgccgtttt
    15541 caccgctggc cagttatctg cagctgcagg cgctgccgtt aagctatttc ccgtggctgg
    15601 ttgcgattct ggcagggtat atgacgttaa cccagttggt gaaagggttc tatagccgtc
    15661 gttacggctg gcagtaaaga ataaactggg catggtggcc cggattcaga ttgcagacaa
    15721 agtgagcttt gttcatgccg gatgcggcgt aaacgcccaa tccggcctac aaattcaata
    15781 aattgcagga atcacgtagg cctgataagc gtagcgcatc gggcgatttt gcttttgtca
    15841 tcagacttaa tccgggcatg atagcccgga tttccatcaa gattagcgac gaacagcgat
    15901 tgcttcgatc tcaatcttca cgtctttcgg cagacgggca acttcaacgc aagaacgtgc
    15961 cgggaaagtg gcgttgtgtt cggtgaagaa ggcttcgtaa gtggcgttta cggttgcgaa
    16021 gtcgttcaga tctttcacga acacagtggt tttaacgatg tcgcccactt tcaggccagc
    16081 ggcttcgacg attgctttta cattatccag cgactgacgt gcctgtgcag cgacgtctgc
    16141 cggtacttca ccagttttcg gatttaccgg gatctgaccg gaggtgatga tcatattgcc
    16201 cagatcaacg ccctgtacgt aaggaccgat agctgccggt gcattttccg tcgcgatagt
    16261 tttgctcatg atttctcctt tattacagcg gtaaaaagtc tggctcatta tagggagcca
    16321 gacttttatt accaaccgca attaattggc cagcaccaca ttatgggaaa actctttttc
    16381 acagtatttg catttaagcg cgatgtcatt ggcgcgtttt cgcacggcaa agctggatga
    16441 aaccggttcg gcatggctga tacagttgct gttcgggcag accagcacat tgtcgatgcg
    16501 ctctggcaga cttgggcgcg atttacccac cacttcatag ttgtcgatac ggttaaccgt
    16561 ggcttgcggt gcatacaatg ccagttggtc tacttgctct tcactcaaaa aggtattttc
    16621 gattttgatc agatctttgc ggcccatctc gccagaaggc aaattcagac caatggtgat
    16681 gcgctgatct gtttcggtca acttgaacag actcaacagc ttaaaaccga tctgggcggg
    16741 gatatggtca attaccgtgc cgcgtttaat agcttcaacc tgcaatttat tatcgtgtgt
    16801 catctctatt tccccttaca gtaccagatc gcgattcaga accagtgcca gtaacgcctg
    16861 gcgagcgaaa atcccgttgc ctgcttgctg gaagtaccag gcgtgaggcg ttttatccac
    16921 atccgtcgca atctcatcaa cacgcggcag cggatgcagc actttcatat tggctttggc
    16981 gttgtggaga tcgctggcgc gaagaacaaa ctgcgctttc acgttggcgt actcggacgg
    17041 gtccagacgc tctttttgca cgcgggtcat gtacaggatg tctacttccg ccatcacttc
    17101 ttcaatagag ctgtgcagac tccaggcgat ccctttttca tcgagcatat ccagaatgta
    17161 ttgcggcatt gccagcgcat ccggcgcgat aaagtaaaaa cggttgccgt cgaacttcgc
    17221 tagcgcctgg gtcagggagt gaacggtgcg gccatatttc aggtcaccaa ccattgcgac
    17281 gtggagattg tccagacgcc cctgggtttc ctgaatggtg aataagtcca gcaaggtttg
    17341 cgtcggatgt tggttggagc catcaccggc attcagtacc ggtacattgc cagaaaattc
    17401 ggtggctagg cgtgccgcac cttcctgcgg atgacgcatc actatcgcat cgacgtaagt
    17461 gctgataacc gaaatggtat cggccagcgt ttcgcctttt ttacccagtg atgtattggc
    17521 gctgtcggag aagcccacca cgctggcacc caggcggtgc atggaagttt cgaaagagag
    17581 gcgggtacgg gtagaggctt cgaagaaaca gctggcaatg actttgtgct tcaacagctc
    17641 tggttgcggg tttgctttca gtttcgccgc tgtcgccagc accagattaa ggtcatcgcg
    17701 actaaggtcg tttatggaaa tgatatgttt ctgatatagc ggattagcca tcttttatct
    17761 cctgacacct aggcaaaaaa aagcccctcg attgaggggc tgggaatggg tgatcaacgg
    17821 gaagaaaaac ggcaggccag cgtctttttt cagacgcggt aagacaaaat gtcgaacaca
    17881 ctgaaccata catcctcccg gcaaattgtc cggcattata ctcatagcca gaagcggtgc
    17941 aagcatttga tgcaatattt tgtcagcgca aacggtttat ttgaattaaa agtcaaaata
    18001 tatgcatttt tattttctat gattctggag gggaagtgtc gttcggtatc aataattgca
    18061 gacatttcct gcttttttct ttttcatttt cacgcaatca gaaatgcatt ttattccatt
    18121 catccgactt atagggcgga gggtgaaaac gaacggctaa cactattgct tatagctcag
    18181 ggatacgcgc tatcacttta atttcaaaat caaagcctgc cagccaggta acacccaccg
    18241 ccgtccagtt tggataaggt ggggcgctaa atatttcatt tttcaccgtc atgatgtctt
    18301 cagattgttt ttccggatcg gtatgaaagc tcgtaacatc aatgatatcg tcaaaagtgc
    18361 atcccgcagc tgccagggtc gcatgcaaat tatcaaaggc cagtctgact tgttgctgaa
    18421 aatcgggttc tggtgttccg tcctctcgac ttcctacttg cccggaaaca aacagcaaat
    18481 cgccggaacg aatagccgca gaataacgat gctcagcata cagtgaatgt cggccagcag
    18541 ggaaaacagc ggttctttct accatttggt tatcctcaag atttacgaca tgaacagaag
    18601 atttatattt atcgggagcc gcttttaggg ggacgacgtt agtaaacaaa acccagacat
    18661 catggataat ggctgggctt aattgagcgt agtcggttat gcgccaaacg cgccatcaat
    18721 ggtatgcatc gcgccggtaa caaaactggc ttctggccct gctaaccatg cgaccatacc
    18781 agcgacctct tccggttgcc catgtctttt gatggccata aaactatgca acatatcgcg
    18841 cattggcccg ttggcgggat taacgtcggt atcaattggt cctggctgga cgacgttgat
    18901 ggtgatccct cgcggtccaa aatcacgggc cagcccgcgc gccatgcctt gcagggcaga
    18961 tttactggcg gcataagcag ccatgcctgc aacaggcata cgatcgccat tcacggagcc
    19021 gatgattaag atgcgcccgc cttcgggcat ctgccgggcg gcttcaacag aggcatgata
    19081 aggagcatga atattgattt tgaaaaggcg atcaatatcg tcggcattta attccagggc
    19141 atcgccaaag acgccaatac ctgcatttac caccaggata tccaatgcgc cgctcttacg
    19201 aacgacatca ataacagcgt ctctgtcagc actatctgtg aatactgctg tcgctccagt
    19261 ctcttgtgcc agacgttcag cggcatcttt cgaccccgca taggtgaatc gtacattggc
    19321 cccatcggtg acgaaacgac gtacgatagc ggcaccgatt ccacgactgc caccgaggat
    19381 gagaactgtc ttaccggtaa aagcgcccat aaggactcct tgatttatta tgtaacatgc
    19441 attacaaaac tgttttaact ttctgtcaac aggttttgta atggtcacta aaaaacaatc
    19501 tcgcgttcca ggtcgtccca gacgtttcgc tcctgagcag gcagtctctg cggcaaaagt
    19561 gctttttcac caaaaaggtt tcgatgcagt aagtgtctct gaagttactg attatcttgg
    19621 tattaacccc ccgagtctct acgcggcttt tggcagtaag gctgggttat ttagccgtgt
    19681 actcaatgaa tatgtcggta cggaagctat tccgcttgcc gatattcttc gtgatgatcg
    19741 tccggtaggc gagtgcctgg ttgaggtatt aaaagaagcg gcgcgcagat atagccaaaa
    19801 cggcggctgc gctggctgta tggttcttga aggtattcat agtcatgatc cacaagcgcg
    19861 tgatattgcc attcaatatt atcacgccgc agaaacgacc atttatgact atatcgccag
    19921 gcggcatcca caaagcgcac aatgtgtgac tgattttatg agtaccgtga tgtcagggct
    19981 ttctgcgaag gcacgggagg ggcactccat cgaacaactc tgtgcaacag ctgccctggc
    20041 aggggaagcg ataaaaactc ttctcaagga gtgatgctgg ccttgatccg aaaggcggga
    20101 acgcgcctgc cgataagttg tgataagaca ataattcacg cgttaaggct ggcggaattg
    20161 atcatctttt cgtataacga tagaaatgaa acattgtttt aattaaggag tggaaaagat
    20221 gatcatcgga aatattcata atcttcagcc gtggctaccc caggagttac gccaggcgat
    20281 tgagcatatc aaagcacacg ttacggcaga aacgccaaag ggcaagcatg atatcgaagg
    20341 caatcgtctg ttttatctta tctcggaaga tatgaccgag ccgtacgaag ctcgccgtgc
    20401 ggagtaccat gcccgctatc ttgacattca gattgtgtta aaaggtcagg aaggcatgac
    20461 cttcagcacg caacctgcag gcgcgccgga taccgactgg ttagctgata aagacatcgc
    20521 atttttgccg gaaggcgttg atgagaaaac agttatccta aatgaaggtg attttgttgt
    20581 gttttatccg ggggaagtgc ataaaccgct gtgcgcagtg ggcgcgccag cccaggttcg
    20641 caaagcagta gtgaagatgc tgatggcgtg atgacttttc gccgtaaata actccaggtt
    20701 tacggcgagt ttgtgaaaag agcgtttttt gatatttttt tgtgagtaaa atttgtaatg
    20761 cttagacgtt cttattaact caaggagttc gtcatgggca aaatatcaga tttgaattat
    20821 tctcaacaca ttacattagc cgacaatttt aaacaaaaaa atgaagcctt agatacctgg
    20881 tatgtgggaa tgaatgattt tgcccgaatt gccggggggc agaatagcag aagcaatatt
    20941 ctttctccta gagcattttt ggagtttttg gcaaagatat ttaccctggg ttatgtggat
    21001 tttagcaaac gctccaacga agcgggtaga aatatgatgg ctcatattga gttctcatct
    21061 tattctaaag atactgatgg caatgaaaaa atgaagtttt acatgaataa tcctgaaggg
    21121 gagcgagcgg atttatctaa ggtgaagata gaaattacac ttgcctcagc ttctactaaa
    21181 gggattcgtg aaggacatac agtcattata tttaaacaat ctgatggttc gactaatcgt
    21241 tatgaaggga agtcttttga aagaaaagat gatagttcat tacacctgat tactaacaag
    21301 gttctggcgt gttaccaacg cgaagctaac aagaaaatag cgcgtctatt aaataatcat
    21361 cagaagttaa ataatataca ggagttaaat gattcgcagg agttaaataa ttcacagaag
    21421 ttaaataatt cgcaggagtt aaataactcg caggtgagtt gtaaaggttc agttgattct
    21481 acgattacgg atttattaga aaaagcattg aa
//
LOCUS       DAJTSI010000063        21469 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000063 DAJTSI010000000
VERSION     DAJTSI010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21469
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.063"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="OO327_002117"
     CDS             complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="OO327_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR6018657.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQAPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQ"
     gene            1794..2540
                     /gene="mipA"
                     /locus_tag="OO327_002118"
     CDS             1794..2540
                     /gene="mipA"
                     /locus_tag="OO327_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR6018658.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2630..3391
                     /locus_tag="OO327_002119"
     CDS             2630..3391
                     /locus_tag="OO327_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR6018659.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3442..4326)
                     /locus_tag="OO327_002120"
     CDS             complement(3442..4326)
                     /locus_tag="OO327_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR6018660.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="OO327_002121"
     CDS             complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="OO327_002121"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR6018661.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5747..6160
                     /gene="msrB"
                     /locus_tag="OO327_002122"
     CDS             5747..6160
                     /gene="msrB"
                     /locus_tag="OO327_002122"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR6018662.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6202..6474
                     /locus_tag="OO327_002123"
     CDS             6202..6474
                     /locus_tag="OO327_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR6018663.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6844..7920
                     /locus_tag="OO327_002124"
     CDS             6844..7920
                     /locus_tag="OO327_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6018664.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7947..9326
                     /locus_tag="OO327_002125"
     CDS             7947..9326
                     /locus_tag="OO327_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6018665.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9343..10388
                     /locus_tag="OO327_002126"
                     /pseudo
     CDS             9343..10388
                     /locus_tag="OO327_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10409..11245
                     /locus_tag="OO327_002127"
     CDS             10409..11245
                     /locus_tag="OO327_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR6018666.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11250..12197
                     /locus_tag="OO327_002128"
     CDS             11250..12197
                     /locus_tag="OO327_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCR6018667.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12207..13187
                     /gene="ydjG"
                     /locus_tag="OO327_002129"
     CDS             12207..13187
                     /gene="ydjG"
                     /locus_tag="OO327_002129"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCR6018668.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13324..14082
                     /locus_tag="OO327_002130"
     CDS             13324..14082
                     /locus_tag="OO327_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6018669.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14200..15558
                     /locus_tag="OO327_002131"
     CDS             14200..15558
                     /locus_tag="OO327_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6018670.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="OO327_002132"
     CDS             complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="OO327_002132"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR6018671.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="OO327_002133"
     CDS             complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="OO327_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR6018672.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAPNYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="OO327_002134"
     CDS             complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="OO327_002134"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR6018673.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19503..20054
                     /locus_tag="OO327_002135"
     CDS             19503..20054
                     /locus_tag="OO327_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR6018674.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20171..21214
                     /gene="selD"
                     /locus_tag="OO327_002136"
     CDS             20171..21214
                     /gene="selD"
                     /locus_tag="OO327_002136"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR6018675.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21219..>21469
                     /locus_tag="OO327_002137"
     CDS             21219..>21469
                     /locus_tag="OO327_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310496.1"
                     /note="decatenates replicating daughter chromosomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR6018676.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTK"
BASE COUNT         5459 a         4939 c         5526 g         5545 t
ORIGIN      
        1 tgctgttcca ggacgtagaa cagatggacc gggtttgccg ccacttctac atgatcgaag
       61 ttgaacacgc gggagaggat cttaaacgca aaacgcgtcg acagaccgtt catcccttca
      121 tcgacaccgg cgtagtcacg atattcctga tacgacttgg ctttcggatc ggtgtctttc
      181 agactttcgc catcataaac ccgcatcttt gaataaatgc tggagttttc tggctctttc
      241 aggcgcgaaa gaatggaaaa acgtgacagt gtttcgagcg tgccaggggc gcatggggcg
      301 tgagtcaatt cactgtgatt aagcaatttc tcgtagattt tgatctcttc ggaaatgcgc
      361 aagcaatacg gcaccttcac gatgtaaaca cggtcgagga aggcttcgtt gtttttgtta
      421 ttacggaaag tgacccattc ggacttgttc gagtgggcga gaataatccc gttgaacggc
      481 agggcggaga taccttccgt cccgttgtag ttaccttcct gagtggcggt taacaaggga
      541 tgcagcactt ttatcggtgc tttaaacatc tcaacgaatt ccatgatccc ctgattggcg
      601 cggcacagcg caccggaata gccgtaggcg tccgggtcat tctgcgcgta gtgttcgagt
      661 ttacgaatat cgactttccc aaccagcgcg gagatgtcct ggttgttctc atcaccgggt
      721 tccgttttgg cgatagcaat ttgttgcaga attgacggcc agaccttcac tacccggaac
      781 ttagtgatat cgccaccaaa ttcatgcagg cgttttgccg cccatggcga catgatggtg
      841 ccgagataac ggcgaggaat gccatactct ttttccagaa tctgagcatc ttcctgcgga
      901 ttgaaaagac agaacggatg atcgttgacc gggctacgct caccgttggc gctcaataca
      961 taaatcggta cgagctgcat taatgatttc agtcgctcag caagcgatga tttaccccca
     1021 cccacaggcc ccagcagata caggatttgt ttcttctctt ccagcccctg agccgcgtgt
     1081 ttcagataag agacaatctg ttcaatcgcg tcttccatgc cgtaaaactc ttcaaatgcc
     1141 ggataacgtg caatgacccg gttagaaaag agtcgagaaa gtctgggggc ctgggctgta
     1201 tcgaccatga caggctcacc gatagccatc aatagccgct cagccgcgtt ggcataagca
     1261 ctgcgatctt gccgacaagt ggtaagaaac tcctgcagtg tgaactcttc gtccttggca
     1321 gcttcatatc gctggcgata gtgatcgaat atattcatgg tatgccgtcc tttcgttttt
     1381 tagcacaggt taagagccgt tcatatgaat agtagaggct cccggaagag gtaaactgaa
     1441 cgacgtctct tgctaaaaca cagcaactct catgccattt tatcgacgat gagaacaacg
     1501 tgcccggtga tacaccttca aaattaagcg tagatggcaa atggaaaact tgcctgcacg
     1561 gctttcttaa tttcaatggc atatcaacaa ctcatccata aattttgcat aattaatgta
     1621 aagaccaggc tcgccagtaa cgctaaatcc atttggctgt aagcgcggtg tcatccgcgg
     1681 caggaaaatt aaacagttac tttaaaaaat gaaaacgtaa aaaggttggg tttcgatgta
     1741 ttgacgggta aactttgtcg cccgctaaac atttgtttaa ggaatgatta attgtgacca
     1801 aactcaaact tctggcactt ggagtgctta tcgcaacgtc tgcaggcgta gcgcacgctg
     1861 aaggtaaatt ttccctgggc gcaggcgtag gtgtcgttga gcacccatat aaagattacg
     1921 ataccgatgt ttacccagta ccggtaatca actatgaagg cgataacttc tggttccgtg
     1981 gcttaggtgg tggttactac ctgtggaatg acgcaacgga taaactttca attaccgctt
     2041 actggtcgcc gctttacttc aaagcgaaag acagtggcga tcaccaaatg cgtcacctgg
     2101 atgaccgtaa gagcaccatg atggctggtc tgtcttatgc tcactttacc cagtacggtt
     2161 acctgcgtac caccctggct ggcgataccc tggataacag caacggcatt gtctgggata
     2221 tggcctggtt gtatcgttac accaacggtg gcctgaccgt gactccgggt attggtgtgc
     2281 agtggaacag cgaaaaccag aacgaatact attatggcgt atcgcgcaaa gagtccgctc
     2341 gcagcggtct gcgtggctat aacccgaatg acagctggag cccttacctt gagctgagcg
     2401 ccagctacaa cttcctcggc gactggagtg tttacggtac cgcgcgctac acccgtctgt
     2461 ctgatgaagt tactgacagc ccgatggtgg ataaatcctg gactggcctg atttctaccg
     2521 ggatcaccta caaattctga taatgcatct ttccagggca ttttgtgcat aaaaacaggg
     2581 cgtcacgccc tgttttgcat tacggtggtg caatacctgg gaggaaacaa tgcaacaaaa
     2641 aatgattcaa tttagtggcg atgtctcact gccagccata gggcagggaa catggtatat
     2701 gggcgaagat gccagtcagc gcaaaacaga agttgctgca ctacgcgcag gcattgaact
     2761 cggtttaacc ctcattgata ccgccgaaat gtatgccgat ggcggtgccg aaaaggtggt
     2821 tggggaagca ttaaccggtc tgcgagagaa ggtctttctc gtctctaaag tctatccgtg
     2881 gaatgctggc gggcaaaaag cgataaatgc atgcgaagcc agtttacgcc gtctcaatac
     2941 tgactatctc gatctctact tattacactg gtctggcagt ttcgcttttg aagagactgt
     3001 tgctgcgatg gaaaaattga tcgcccaggg aaaaatccgt cgctggggcg tttctaacct
     3061 tggttatgcc gatatgcagg aactctggca gctgccgggg ggaaatcagt gtgccactaa
     3121 tcaggtgctt taccatctcg gttcacgagg gattgagtac gatctactcc cctggtgcca
     3181 gcaacagcag atgccggtga tggcttacag tccattagcc caggccgggc ggttgcgcaa
     3241 tggactgtta aaaaacgcgg tagtcaacga aattgcacat gtccaacaac atgcagctgc
     3301 gcttgaggtc gaactttcgt cagcggaatt agctatgctg gataaggcat atccggcacc
     3361 aaaaggaaaa actgcgctgg atatggtgtg atgttgatac gtgccggatg catcgcaccc
     3421 ggcacacaac attaacgcaa attaacgttt ggcaacgcga atggattgcg ccagatgtgc
     3481 aggtttctct ttggtgactt tttgcgtttc tgaagcgtaa gccgtttcta cacaaacaaa
     3541 ggtcttgtag ccatcgtccg gcatgtcgcc catgctaact gaaagcgccg gtcccgggtt
     3601 ccagccgaca acgttcagat gatgctggtg gcctacggcg ataatacgat tcagcgcttc
     3661 atcattaatc acgctgcaat cttgcggatt cagatacacg cgatcggtac ggtcagggaa
     3721 ggtctgaata ccgtcggtca gtacatcttc tttcgcgtca ttcactttat caatgaagcg
     3781 atcgcccagc ccactgacgc ttaccttagc gatatcaccc acgttaaagt aggtatgcag
     3841 ggcagaggtg gtttcaaatt cgccatgtga ttcaagatcg atttcacagg ttttacccac
     3901 gcggaaatgc gctaacagcg taaagtcgtg cggccagaat tttttcgtct cttcgctttg
     3961 tgtcaattca aaagtcagcg ctacgccatc agcatcttca tgatgtgatt tcagcgtcca
     4021 cggcaggttg cgggcaaaac cgtgcgcagg cagaccttgt tgtgccgccg gaccaaacca
     4081 cggccagcaa accggtacgc caccgcgaat agcgacgcca tttttgaacg gtgtgttgtt
     4141 gctcaaccac agaacttctt cttcacccgc aggtttccac gagagaaggt gtgcgccctg
     4201 taatgcaaaa gaggctttta cctggggatg atcgaccaca atgaggtcca gttcatccag
     4261 tttacgacgg gagaggacag gggagatttg ttcgatgacc ggaagggcaa aaattttctt
     4321 aatcatgacg cagtccttta acttcatttt atcaggtaaa aaaaagagcg accgaagtcg
     4381 ctctttttag atcacagtgt catctcaact tatttggaga tgtgagcgat caggtccaga
     4441 actttgttgg agtaaccggt ttcgttgtcg taccaggata ccagtttcac gaagttgtcg
     4501 ttcagagcga taccagcttt agcatcgaac acggaagtgc aaacttcgcc gttgaaatcg
     4561 gtagatacta cgtcatcttc ggtgtagccc agaacgcctt tcatttcgcc ttcagcagca
     4621 gctttaacgg cagctttgat ctgctcgtaa gttgcagctt tttccagacg aacggtcagg
     4681 tcaactacgg atacgttcgg ggtcggaacg cggaacgcca taccagtcag tttgccattc
     4741 agttctggca gtactttacc tacagcttta gcagcaccgg tagaggacgg gatgatgttc
     4801 tgggaagcgc cgcggccgcc gcgccagtct ttgtgagacg ggccatcaac ggttttctga
     4861 gtagcggtag tagcgtgaac ggtggtcatc agaccttcga tgatgccgaa gttatcgttg
     4921 ataactttag ccagcggagc caggcagttg gtggtgcagg aagcgttgga aacgatgtcc
     4981 tggccagcat atttgtcgaa gttagcgcct ttaacgaaca tcggagtgtt gtctttagac
     5041 ggaccagtca taaccacttt cttcgcacca gcggtgatgt gtttacgagc agtttcgtca
     5101 gtcaggaaca gaccagttgc ttcagcgaca acgtcaacac ccacttcgtc ccatttcagg
     5161 ttagccggat cacgttcagc ggtaacacgg atttttttac cgttaacgat cagatgaccg
     5221 tctttcactt caacggtacc gtcgaaacgg ccgtgagtgg agtcatattt cagcatgtat
     5281 gccatgtaat cagcgtctaa caggtcgttg attgcaacga tctcgatgtc agaacgtttc
     5341 tgagcagcac ggaaaacaat gcgaccgata cggccaaaac cgttgatacc tactttgata
     5401 gtcatatatt ccaccagcta tttgttagtg aataaaaggt tgcctgtaaa attacaaaaa
     5461 ccttacgcag cgtcaagcgg aatcgtgtca atcattgcga caaatcaatc ctgtgcctaa
     5521 gcattacgcg actgactcgc ctcacttttc ctttgggctt gagaccacat ggggtcggcg
     5581 ccccgaattt taaagggcaa ttacgataaa aatgtgattt tcatcacaat ttaggtgcag
     5641 ccatttcgtc gagatcaagt ttaggacaaa attttgccag ttggtgttaa tgttttgtta
     5701 gaatcagtca cgtaatgtga gcacgattaa agtgagatgt gagtaaatgg cgaataaacc
     5761 ttcggcagaa gaactgaaaa aaaatttgtc cgagatgcag ttttacgtga cgcagaatca
     5821 tgggacagaa ccgccattta cgggtcgttt actgcataac aagcgtgacg gcgtatatca
     5881 ctgtttgatc tgcgatgccc cgctgtttca ttccgaaacc aagtatgatt ccggctgtgg
     5941 ctggcccagt ttctacgaac cggtgagtga agaatccatt cgttatatca aagacttgtc
     6001 acatggaatg cagcgcatag aaattcgttg cggtaactgt gatgcccatc tggggcatgt
     6061 ctttcccgac gggccgcaac caacaggcga acgttattgt gttaactctg cttctttacg
     6121 cttcactgat ggcgaaaacg gcgaagaaat caacggttga aaaaacgatt cagcaactta
     6181 ttccacagga gcggaaaaat tatgaatctt gatgacatta tcaacagcat gacgcctgag
     6241 gtataccagc gtttgtcgac cgccgttgaa ctggggaaat ggcctgatgg cgttgcgttg
     6301 accgaggaac aaaaagagaa ttgcctgcaa ctggtgatgc tgtggcaagc ccgccataat
     6361 accgaagcac agcatatgac aattgacaca aatggtcaga tggtcatgaa gagcaaacag
     6421 cagttaaaag aagattttgg tatctcagca aagcctattg ccatgtttaa gtaagtggtt
     6481 catccccttt ttgccgcggc tatgaagggg atagccaaca atgaccccac gactcgtctc
     6541 aattatctcg acccgacctg gctaagtccc agtccgatct cttgcagtcg cgttcacgcc
     6601 tgaatgactt ctcctgaact taagtctcag acctatttgg ccggtaatcc ctctcgaatt
     6661 tgctcttttt gcgtcccgcc ttccaccaac ctgtaacaga cccagccatt ttctgcgtaa
     6721 ttgtgatttg agttgtaaaa tgcaggtcat tatctgtgtt gtgcagattt aatattgacc
     6781 tcaaaaatca tgtgggtaag tatgatggcc atctaagcaa aacaaaacac agaggatgac
     6841 gcaatgaaag cactggctcg gtttggcaag gcctttggcg gctacaagat gattcatgtc
     6901 ccacaaccca tttgtggccc ggaagacgta gtgactgaaa ttaaagccgc ggcaatctgc
     6961 ggcgcagaca tgaagcacta caatgtcgat agcggttctg atgagtttaa ctctatccgc
     7021 ggccatgagt tcgcaggttg tattgcgcag gttggtgaaa aagtcaaaga ctggaaagtg
     7081 gggcaacgcg tcgtgtcgga taacagcggt cacgtttgcg gcgtttgtcc ggcctgtgaa
     7141 cagggtgatt ttctgtgttg tacagaaaaa gtaaaccttg gtctggataa caatacctgg
     7201 ggcggtggtt tttccaaata ttgtctggtt cctggtgaaa ttctcaaaat tcatcgtcat
     7261 gcgttgtggg aaatccctga tggtgttgat tatgaggacg cagccgtact tgaccctatc
     7321 tgtaatgcct ataaatccat cgcgcagcaa tcgaaattcc ttcctggtca ggatgttgtc
     7381 gtcattggca ctggcccact cgggctgttt tccgtacaaa tggcgcggat tatgggggcg
     7441 gtaaatatcg tcgtcgttgg tctgcaagaa gatgtggcgg tccgcttccc ggttgcaaaa
     7501 gaactggggg cgacggcagt agtaaatggt tctaccgaag atgtggtggc gcgctgccag
     7561 caaatttgtg gcaaagataa tctgggactg gtgattgaat gctccggtgc caatatcgca
     7621 ctgaaacaag ccatcgaaat gctccgcccg aatggggaag tggtacgcgt tggaatgggc
     7681 ttcaaacctc ttgatttctc gattaatgac attaccgcct ggaataaaag catcattggg
     7741 catatggcct atgactccac ctcatggcgt aacgctatcc ggctattagc cagcggcgct
     7801 atcaaagtca aaccgatgat cacgcatcgt atcggcctgt cgcaatggcg cgaagggttt
     7861 gatgcgatgg tcgataaaac cgcaatcaaa gtgatcatga cttacgactt tgatgaataa
     7921 ctactctcct gaaggaacgt tatcgcatgg aacagataac aaaaccgcat tgtggtgcac
     7981 ggctggatcg cttaccggat tgccgctggc attcgtcaat gtttgccatc gtcgcgtttg
     8041 gcttgcttgt ctgctggagt aatgccgttg gtggcttgat cctcgcgcag ctgaaagcgt
     8101 tgggctggac agataattcc actacagcca cattctcagc aatcacgacc gccggaatgt
     8161 ttctcggtgc gctggtgggc ggcatcattg gcgacaaaac cggtcgcaga aatgcgttca
     8221 tcctctatga ggccattcat attgcctcga tggttgtcgg tgctttctca ccgaatatgg
     8281 attttcttat tgcctgccgt tttgtgatgg acgttgggct gggagcttta ctggttacac
     8341 tgtttgctgg tttcaccgaa tatatgcccg gtagaaatcg cggaacgtgg tccagtcggg
     8401 tttcctttat tggcaactgg tcatatccgc tctgctcact gattgcgatg ggactcacgc
     8461 cgctgattag tgcagagtgg aactggcggg tacaactgct tattcctgca atattgtcgc
     8521 ttatcgctac ggcgctggcc tggcgctact ttcctgaatc cccgcgctgg ctggaatcgc
     8581 gcggacggta tcaggaagcc gagaaagtca tgcggagtat agaagaaggc gtcatacgcc
     8641 agacgggtaa acctttgccg cctgtggtta ttgctgatga cggtaaagcg ccacaagcgg
     8701 tgccgtattc agccttactg acaggagtat tactgaaacg cgtgatatta ggctcttgtg
     8761 tgctgattac catgaacgtt gtgcagtaca cactaattaa ctggttgcca acaatattca
     8821 tgacccaggg gattaattta aaagactcga ttgttttaaa taccatgagt atgtttggcg
     8881 cgccatttgg tatttttatt gccatgctgg tgatggataa aattccgcgt aaaacaatgg
     8941 gtgtggggct attaatcctg attgcggtgc tcggatatat ctattcactg caaaccagta
     9001 tgttgctcat aacgctgatt ggtttcttcc tgattacttt cgtctatatg tacgtttgct
     9061 atgcctcggc agtgtatgtc cctgaaatct ggccgacaga ggccaaactc cgtggctccg
     9121 gtctggcgaa tgcggtaggg cgaatcagtg gtattgccgc accttatgcc gttgcagtgc
     9181 tgctcagtag ttatggtgta acgggagtct ttattcttct gggggctgtt tcaaatattg
     9241 tcgcaattgc tatcgccacc attggaattg aaaccaaagg tgtctccgtt gaaagtttaa
     9301 gtattgatgc agtagccaat aaataatacg ggtggattca aaatgaaaaa ttcaaaagca
     9361 atattgcagg tgccgggcac aatgaaaatt atttcagcag aaataccagt gcctaaagaa
     9421 gatgaagttt tgattaaagt agaatatgtc ggtatttgtg gttcagatgt acatggtttt
     9481 gaatcaggcc cgtttattcc gcctaaagac ccaaatcaag aaattggcct gggtcatgaa
     9541 tgcgccggga cggttgtggc tgtgggaagc cgcgtgcgca aatttaaacc gggggatcgg
     9601 gtaaatatcg aacctggcgt tccttgcggt cactgtcgtt actgtctgga aggcaaatat
     9661 aacatctgcc cggacgttga ttttatggcg acacaaccca actaccgcgg cgcattaacg
     9721 cactatctgt gtcatccgga gagctttact tacaaactgc ccgacaatat ggacacgatg
     9781 gaaggggcgc tggtggagcc tgccgcagtc gggatgcatg ccgcgatgct ggcagatgtt
     9841 aaaccgggta agaagataat tattctggga gcaggttgta ttggtttgat gacgttgcaa
     9901 gcgtgcaaat gcctgggcgc aacggaaatt gccgtcgttg atgtactgga aaaacgtctg
     9961 gcaatagggg aacagcctgg tgcgacagtg gttattaatg gcgcaaaaga ggacactatt
    10021 gcacgctgtc agcaatttac cgaagacatg ggcgcagata ttgttttcga aacagcgggt
    10081 tctgcggtca ccgttaaaca ggcaccttta atctggtaat gcgcggcggt aaaattatga
    10141 ttgttggtac tgtacccggc gattcggcaa tcaatttcct caaaatcaat cgcgaagtca
    10201 ctatccagac ggtattccgc tatgccaatc gttatccggt cacgattgaa gctatttctt
    10261 cagggcgatt cgatgtgaaa tcgatggtga cgcatattta cgattatcgg gatgtacaac
    10321 aggcatttga agagtcagtt aacaacaaac gcgacattat taaaggcgtt attaaaatta
    10381 gcgattaatt aatacgggag aacagaacat gctcgcagat atcagatatt gggaaaacga
    10441 tgccacaaat aaatattatg caattgccca ttttaacgta tggaatgcag aaatgttgat
    10501 gggcgttaaa gacgcagccg aagaagcgaa atccccggtt attatttctt ttagtacagg
    10561 ttttgtcggt aacacctcat ttgaagattt ctctcacatg atggtatcaa tggcacaaaa
    10621 agcaacggtg ccggtaataa ctcattggga tcatggtcgg agtattgaga ttattcataa
    10681 cgcctggact catggtatga attcattaat gcgtgatgct tctgcatttg atttcgaaga
    10741 aaatattcgt ttaaccaaag aggctgttga tttctttcat ccgctgggta ttccggtaga
    10801 ggcggaatta gggcatgtcg gtaatgaaac cgtttatgag gaagcgctgg cgggctatca
    10861 ctataccgat cctgaccagg cggctgaatt tgttgaacgt acgggctgtg actcattggc
    10921 tgtcgccatc ggcaaccagc atggggttta tacgtcagag ccgcaattga actttgaggt
    10981 cgtcaaacgc gtacgcgatg ctgtttctgt tccgctggtt ttgcatggcg catcggggat
    11041 cagtgatgcc gacattaaaa ctgcaatttc gttgggtatt gcgaaaatca acattcatac
    11101 ggagctctgt caggccgcaa tggtagcagt gaaagaaaac caggatcagc ctttcctgca
    11161 tttagaacgt gaagtacgta aagctgtaaa agaacgggca ttggaaaaaa ttgaactgtt
    11221 cggttctgat ggcaaagcgg aataatgata tggataatct cgacgttatt tgtataggtg
    11281 ccgctattgt tgatattcca ttacaaccgg tcagtaaaaa tatctttgat gtagattctt
    11341 atcctcttga aagaatcgca atgagcaccg ggggcgatgc aataaatgaa gcaacaatta
    11401 tttctcgtct ggggcatcgt accgcattaa tgagccgcat tggtaaagat gcctcagggc
    11461 aatttattct cgaccattgt cgcaaagaga atattgatat tcaaagcctg aaacaggacg
    11521 tcagcataga tacatctatt aacgttggac tggtgacgga ggatggcgag cggacgtttg
    11581 tcacgaaccg taacggcagt ttgtggaaat taaatattga cgatgttgat tttgcgcggt
    11641 ttcctcaggc gaaattatta tcactggcca gtattttcaa cagtcctcta ttggatggta
    11701 aagcgctaac agaaattttt acgcaagcga aagcccggca gatgatcatt tgtgccgata
    11761 tgatcaaacc gcggttgaat gaaacgctgg atgatatttg cgaagcattg agttatgtcg
    11821 attatctgtt tccaaatttt gccgaggaaa aattactcac agggaaagag acactggatg
    11881 aaattgctga ctgctttctt gcgtgcggcg taaaaacggt ggtgattaaa acgggtaaag
    11941 acggctgctt tatcaagcgt ggtgacatga cgatgaaggt gccggcggtc gcaggaataa
    12001 ccgccatcga caccattggc gcgggcgata actttgcttc aggttttatt gcggcactgt
    12061 tagaaggcaa aaatctgcgt gaatgcgcac gctttgccaa tgcaacggcg gctatctcgg
    12121 ttctaagcgt cggtgccacc accggcgtaa aaaacagaaa gctggtcgaa caattgctgg
    12181 aagaatacga aggataatga aggcaaatga aaaagatacc tttaggcacg acggatataa
    12241 cgctttcgcg aatggggttg gggacatggg ccattggcgg cggtcctgca tggaatggcg
    12301 atctcgatcg gcaaatatgt attgatacga ttcttgaagc ccatcgttgt ggcattaatc
    12361 tgattgatac tgcgctagga tataactttg gcaatagtga agttatcgtc ggtcaggcgt
    12421 taaaaaaact gccccgtgaa caggttgtag tagaaaccaa atgcggcatt gtctgggaac
    12481 gaaaaggaag tttattcaac aaagttggcg atcggcagtt gtataaaaac ctttccccgg
    12541 aatctatccg cgaagaggta gaagccagct tgcaacgtct gggtattgat tacatcgata
    12601 tctatatgac gcactggcag tcggtgccgc cattttttac gccgatagct gaaactgtcg
    12661 cagtgcttaa tgagttaaag gctgaaggga aaattcgcgc tataggcgct gctaacgtcg
    12721 atgctgacca tatccgcgag tatctgcaat atggtgaact ggatattatt caggcgaaat
    12781 acagtattct cgaccgggca atggaaaacg aactgctgcc gctatgtcgt gataatggca
    12841 ttgtggttca ggtttattcc ccgctagagc agggattgtt gaccggcact atcactcgtg
    12901 attacgttcc gggcggcgct cgggcaaata aagtctggtt ccagcgtgaa aacatgctga
    12961 aagtgattga tatgcttgaa cagtggcagc cactttgtgc tcgttatcag tgcacaattc
    13021 ccactctggc actggcgtgg atattaaaac agagtgattt aatctccatt cttagtgggg
    13081 ctactgcacc ggaacaggta cgcgaaaatg tcgcggcact gaatatcaac ttatcggatg
    13141 cagacgcaac attgatgagg gaaatggcag aggccctgga gcgttaaata ctcaacacga
    13201 ttcacctctg tttctgtgaa aactgtgatg aaaatctgtg attcaaatag gttattttga
    13261 aagtaaacat cggttatgcg ataatcgcgc taatgtgatg taaaaagata caggggtgta
    13321 atcgtggcgg caaaagacag gattcaggca attaagcaaa tggttgccaa cgataaaaag
    13381 gtgacagtct caaatttgag tgggattttt caggtaaccg aagaaaccat tcgccgcgat
    13441 cttgagaagc tggaagatga aggctttttg accagaacct atggtggtgc tgttttaaat
    13501 acagcggtgt tgacggagaa tatccatttt tataagcgcg cttcatcgtt ttatgaagag
    13561 aagctgctta ttgcacgcaa ggcactaccc tttatcgaca ataaaaccac catggcagcc
    13621 gattccagta gtaccgtaat ggaattgctc aaattgttac aggaccgtag tgacctgacg
    13681 ttgctaacca actcggcgga agcgattcat gttctggctc agtcagaaat taaagtcgtt
    13741 tcaacgggtg gggaactaaa caaaaataca ctttccctgc aaggaagaat tactaaagag
    13801 atcatcagcc gctaccatgt cgatatcatg gtaatgagtt gtaaaggtct tgatattaac
    13861 agcggcgcgc tggactctaa tgaagcggaa gctgaaatca aaaagacaat gatccgtcag
    13921 gcgacagaag ttgcgttatt ggttgatcac tctaagtttg atcgtaaagc ttttgtccag
    13981 ttagctgact ttagtcatat taattatata ataactgata aatcaccggg tgcagagtgg
    14041 atagcatttt gcaaagacaa taatatccag ctggtgtggt aatttaagca cacgttgtca
    14101 atattatttg ttcctgcgac tggaaataaa atgatgataa agatgcggca agtgaatttg
    14161 ccgcaattgc taccttttat agcaaatccg aggcacccaa tggaacaata tgatcaaatt
    14221 ggcgcaagac tggaccgctt gcctttggcc cggtttcatt atcgtatatt tggtataata
    14281 agctttagtc tgttattaac ggggtttttg agttactcag gtaatgtcgt cttagcaaag
    14341 ctggtaagca atggatggtc aaataatttc ctctatgccg cctttacctc ggcattaatg
    14401 tttggttatt tcatcggctc acttactggt gggtttattg gtgactactt tgggcggcgc
    14461 agggcgtttc gcataaatct tctcatcgtc ggtattgctg caacaggggc cgcttttgtc
    14521 cctgatatgt actggctcat tttctttcgc ttcctgatgg gaacaggaat gggggcgctg
    14581 attatggttg gctatgcctc atttacggag tttatccccg cgacggtgcg tggaaaatgg
    14641 tccgcgcggc tctcatttgt tggtaactgg tcgcccatgc tgtctgcggc gataggcgtg
    14701 gtggttatcg ctttttttag ttggcgaata atgtttcttc tgggtggtat tggcatactg
    14761 ttagcctggt ttctctcagg taaatacttt atcgagtcgc cacgatggct ggaagggaaa
    14821 gggcaaatcg ccggtgcaga aagccaactt cgtgaagtag agcagcaaat tgaaagggag
    14881 aagagtattc gtttacccca gcttactttg aaccagagca acagcaaggt taaggtaatc
    14941 aagggtacct tcaggctcct gtttaaaggg gaaatgttac gacgtacatt agttgcgata
    15001 actgttttaa ttgcaatgaa catttcgctt tataccatta ccgtatggat accgaccata
    15061 tttgttaact ccggcattga tgtcgataaa tcaatattaa tgaccgctgt tattatgatt
    15121 ggtgctccgg taggaatatt tattgcggca ttaattattg atcattttcc tcgtcgatta
    15181 tttggctccg ccttacttat tattattgcc gtgttaggct atatctattc aagtcagact
    15241 acagagtggg cgattttaat ctatggtctg gtgatgatct tctttttata catgtatgtt
    15301 tgcttcgcgt cggcggttta tatcccggag ctttggccaa cgcatttacg cctgcgcggt
    15361 tcgggtttcg ttaatgccgt cggacggatc gtcgcagttt tcacgcccta tggcgttgcg
    15421 gcattattaa cacattatgg gtcgatcacg gtgtttatgg tgcttggtgt catgttagtg
    15481 ctctgtgcgc tggttctctc catttttggc atcgaaacgc ggaaggtgtc gctggaagag
    15541 atttctgagg tgaattaaat ctttcgttga tggcatcaat tgccagatgt accactgacg
    15601 tgttttattt ggcctacaat gccagtacag aattgtaggc cagcgtaaaa ttacccctgc
    15661 gtctcttccc agtctgccag cgtatatagc gttgccccag ctgctgacat ctccataaac
    15721 gcgtgtgcac tgtcctgggg ctggatattc acgccacgac agccatcggt aatcacgttt
    15781 accttataac ctaactgtaa cgcgtccagc acggtaaact tcacgcaata gtcagtagcc
    15841 aggcccataa cgatcaattc atcgatttca tgatcgcgca accagtcatc aagcgcggtt
    15901 ttctgccgac gtccgttatc aaaaaaggca ctgtaactgt caactaaagg attttcgcct
    15961 ttatggaaca ccgctgcgat ctctttttga ttcagtaacg gatgtaattg tgcgccttca
    16021 ctgttctgca cacagtgttc aggccagaaa gtttgccgca ggccgccgag ttggcctggc
    16081 gtataaggct ctacgccgtg ctggctggca aagctgccgt gattcgccgg atgccagtcc
    16141 tggctggcga taaccgcttc tccgcgcgac tggcaccagt caataaggcg gttagcaacc
    16201 tccaccgtac tgtcaccttc cggtacggcg agcgcgccac cagcacagaa atcattttgt
    16261 aaatcgacca gtaacagggc gcgatggggc attgcagtct ccttaatcat ccggcgtcag
    16321 ttcgccgcgc aggttttggc tcatggcctt gcgaatggtt tcagtatcca gttcctggct
    16381 cagcaggtaa tgcagtttgg ttagcgtggc ttctacagtc atatctgcac cgtcaataac
    16441 gccggcatgg gcgagggcgt taccggtggc ataaccactc atattcactt taccggacat
    16501 acattgtgtc aggttgacca ccacaatacc gcgatcgctg gcttcttgta attcctgcag
    16561 gaaggctttg ttttgtggcg cattacccac gccataggag cgcagaatca atgctttcac
    16621 cggttggcgc agaaaattgc gcacgacgtc agcagaaatc cctggataaa tcgtcaccac
    16681 gccaattggt tgtggcgtga ttggatgaac gattaattcc ccttcaccgt gcggggcggg
    16741 tggcgtattc aaacgacgaa tatggatacc agcttccagt aacggaggaa ggtttggaga
    16801 ggcaaacgca tcaaaaccat cggcatgggc tttggtagtg cggttgccgc gatacaatcg
    16861 gttattgaaa aagagcgtta cttcgttgat cggataattc ggcgcaacgt acaacgcatt
    16921 cagcagatta atttgtccgt cagagcgtaa ctcagccagc gggatttgtg accctgtcac
    16981 aatgaccggt ttaccgagat tctcgagcat gaacgacagc gcagaggcgg tatacgccat
    17041 cgtgtcggta ccgtgtagaa tgacaaaacc atcatagtcg tcatagtgcg ctttaatatc
    17101 ttcagcaata tgctgccagt cttccggcgt catatcagaa gaatccatca acggcgtata
    17161 ttcatgaatg gtgaaatctg gcatctccgg gcgatggaat tccggcatca gcgccagttg
    17221 gcgttgtaga tgacctgaca ccggaatata accctgctcg gaacgctgca tcccgatggt
    17281 cccgcccgtg taggcgacgt aaattgattt cttttgcatg atattgatat gaatgatatc
    17341 gaaagagcaa aagtatatac gtgaggcagg aggagcgcac ctgatactat gcggataagg
    17401 aaatatgggg cgagacagcg ggaagaggat tcgtcgaggt aaaaaataat gtggccgacg
    17461 atctgtcggc cactcagtac aagacttaac gcacgttggc gcaggtcagg caaaacgcat
    17521 aacggttttg tgggtcgtta aacgcggcca gcttgtcgct ttcactttta acggtagagg
    17581 ctaccgaggc aagcggtgca ggtaacatgg cctggaacgc atctggcaac attgcccgga
    17641 cagaaccaga catgttgtcc atcactttgt cgaagaaggt cggttcatca acgtagtatt
    17701 ccagatgcca ctgtttcact tttgcgagct ctgctgcttt ggcaaccgca tcatcgaaat
    17761 ccccgagact atcgaccagc ccgttagctt ttgcatcctg accggtccag acgtggccct
    17821 gggcgatttt atcaatttgc tccggcgtcg aatgacgcgc atcagcaacc agcgtgataa
    17881 agcgtttata gccattctca atgcttaatt gcatcatctg ctgcgcttcc ggcggcagtg
    17941 ccctggtgat agaaacatcc gccagcggtg aagttgagac accatcagta tgaacaccaa
    18001 tcgaatccag actattttct acggtggtga tcacgccgaa gataccgata gaaccggtca
    18061 gggtgctggg gttagccaca atgtaattag ctggcgtgga aatccagtaa ccaccagatg
    18121 ccgccatgcc gcccatcgat acaaccacag acttacccgc tgcccgggct gctgccagtt
    18181 cagcgcgaat cacttcagac gcggtaacgc tgccgcctgg gctattaaca cgcaggacaa
    18241 tcgctttcac tttcgggtca aggcgagcgt cgcggatttg tgccgcagtg gtatcaccgc
    18301 caacattccc ctgagtttcc tcgccatcca taattgcgcc attagcgaag acgacaccga
    18361 tgctgtcacc ggtatctgcc ggcgttttca atgcgtaatc gtaataactg atggcgcgat
    18421 aatttttatc agtcttactc cagccgaact ctttggtcag agctttttcg atttcggcac
    18481 tcgatgccag tgcatcgacc agcttgtttt ccagtgcata tttcgcggta tcgccaccgg
    18541 ttttggttaa accctcaagc aacccttgcg cgccagggaa tacctgctga gcagggatct
    18601 gccggttagc ggcaacagta ttcagatagt tttgccacag ctcaccaatc cagcggctgt
    18661 cagcttcgcg ggctgccggt gacatatcat cacgaataaa cggttcaacg gcagatttat
    18721 acgtacccac gcggaacaca tgggtggaaa ctttcagctt atccagcaac gatttgtagt
    18781 acagaccgtt ggtagcaaaa ccgtgcagat caaccacgcc ttgcggtgac agccaaattt
    18841 tattggcgaa actggcgaga taatattgcc cctggctgta gttctcgcca acggcataaa
    18901 ccggtttccc gctgtcacga aactctttca gggctttgcc gatgtactgc atagacggct
    18961 ggtcgccgcc tgcgaagttt ttcagatcca tcacgatacc ggtgatattg cggtcgtcct
    19021 tcgcctggcg aatggtgttg acgatatcaa acagtgagtt ttcctgcaga cgatcggaac
    19081 tggcaccaag cagctggcgg cttaatttac taaaccgctg agaactgtct ggtttatcga
    19141 cgatcacacc agaaatgtcc agcagcagtg cgccacgact ggccgtttct ttcgaatcac
    19201 caccactgac ctgcatccag atccccacac caaccagtac gaggaaaata aagaacaggt
    19261 taagtaccat ttcacggacg aaattcagca gacgccacgt ccatttaaaa aatccggcaa
    19321 taaatcgcca aagggttcgc atgtattctc ccaacttaag gtcacacctg tcacaggcgc
    19381 aatagatgtg gttatcgtaa tgacccaacc ggcacttgtc agcaggaatt gcggactgtg
    19441 ctgtaacaaa atccactctc gtgttaattt tgtgaataaa tatcacgaca ggagttaatc
    19501 aaatggatgc actcgaacta ttgatcaatc gccgtagcgc ctcccgcctg gctgaacccg
    19561 cgccaacggg tgaacaactg caaaacatcc tgcgtgcggg tatgcgtgcg ccggaccata
    19621 agtccatgca accgtggcat ttttttgtga ttgaagggga agggtgcgag cgtttcagcg
    19681 ccgtactgga acagggggcg attgctgccg gtagtgatga taaagctatc gacaaagccc
    19741 gtaatgcgcc gttccgcgca ccgctcatca tcacggtggt ggcgaaatgc aaagaaaatc
    19801 ataaagtccc gcgatgggaa caggaaatgt ctgccggatg cgcggtcatg gcgatgcaaa
    19861 tggcagccgt tgcccaggga tttggcggca tctggcgcag tggcgcatta actgaaagtc
    19921 cggtagtgcg tgaagcattc ggttgccgtg agcaggataa aattgtcggt tttctctacc
    19981 tcggcacgcc gcagttgaaa gcatctacgt cgattaacgt cccggacccg acgccgtttg
    20041 taacttattt ctgataattg tcgcgaaact gtctggattg tgagcaaagg cgcaggaatt
    20101 cagacactct cacttatcac ttcacggaat gagggttacc atacccggat tgcattgaca
    20161 ggagatgtcc atgagcgaga actcgattcg tttgacccaa tacagccacg gagctggttg
    20221 cggctgtaaa atttccccaa aagtgttgga aaccatcctg cacagtgagc aggcgaagtt
    20281 tgttgatccg aatttgcttg tgggtaatga aacccgcgac gatgcggcgg tgtacgatct
    20341 gggcaatggc accagcgtta tcagtaccac cgacttcttt atgccgatcg ttgataatcc
    20401 tttcgatttt ggccgcattg cggcgactaa cgccatcagc gatatcttcg cgatgggggg
    20461 caaaccgatt atggcgattg cgatcctcgg ctggccgatt aacaaacttt ccccggaaat
    20521 tgcccgcgaa gtaaccgaag gtggacgcta tgcatgccgt caggcgggta ttgcgctggc
    20581 tggcggtcac tccatcgatg cgccggagcc gatttttggt ctggcggtaa cggggatcgt
    20641 accgaccgag cgggtgaaga aaaacagtac cgcacaagcc ggatgcaaac tgttcctgac
    20701 gaaaccgctg gggatcggcg ttcttaccac ggctgagaaa aaatcactgt tgaaaccaga
    20761 acatcaggga ctggcgacgg aagtgatgtg ccggatgaac atcgcaggcg cgtcctttgc
    20821 caacatcgaa ggcgtaaaag cgatgaccga cgttacgggc tttggtctgc tgggccactt
    20881 gagcgaaatg tgtcaggggg ctggtgtgca ggcacgcgtc gactatgaag cgatcccgaa
    20941 actccccggt gttgaagagt acattaagtt gggcgcagta cctggcggca ctgaacgtaa
    21001 ctttgccagc tacggtcatc tgatgggtga aatgccgcgt gaagtgcgcg atctgctgtg
    21061 cgatccgcaa acttctggcg gtttgctgct ggcggtcatg ccggaagcag aaaatgaggt
    21121 taaaactaca gccgccgagt ttggcattga actgacggcg attggcgaac tggtgccagc
    21181 gcgcggcggt cgtgccatgg ttgagatccg ttaatttaat gcggttgttt attgccgaaa
    21241 aaccgagtct ggcgcgcgcc attgctgatg tcctgcccaa accgcaccgg aaaggcgatg
    21301 gctttatcga gtgcggtaat ggtcaggtgg tgacttggtg tatcggtcac ctgcttgagc
    21361 aggcgcagcc agacgcctac gacagccgct atgcgcgctg gaatcttgcg gatttgccga
    21421 ttgtcccgga aaagtggcaa ttacagccac gaccctccgt gaccaaaca
//
LOCUS       DAJTSI010000064        21253 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000064 DAJTSI010000000
VERSION     DAJTSI010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21253
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.064"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            18..791
                     /locus_tag="OO327_002138"
                     /pseudo
     CDS             18..791
                     /locus_tag="OO327_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445615.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(851..2149)
                     /gene="eno"
                     /locus_tag="OO327_002139"
     CDS             complement(851..2149)
                     /gene="eno"
                     /locus_tag="OO327_002139"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR6018677.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2237..3874)
                     /gene="pyrG"
                     /locus_tag="OO327_002140"
     CDS             complement(2237..3874)
                     /gene="pyrG"
                     /locus_tag="OO327_002140"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR6018678.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4102..4893)
                     /gene="mazG"
                     /locus_tag="OO327_002141"
     CDS             complement(4102..4893)
                     /gene="mazG"
                     /locus_tag="OO327_002141"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR6018679.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4989..7223)
                     /locus_tag="OO327_002142"
     CDS             complement(4989..7223)
                     /locus_tag="OO327_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR6018680.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7271..8572)
                     /gene="rlmD"
                     /locus_tag="OO327_002143"
     CDS             complement(7271..8572)
                     /gene="rlmD"
                     /locus_tag="OO327_002143"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR6018681.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8629..11385
                     /gene="barA"
                     /locus_tag="OO327_002144"
     CDS             8629..11385
                     /gene="barA"
                     /locus_tag="OO327_002144"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR6018682.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11617..12957)
                     /gene="gudD"
                     /locus_tag="OO327_002145"
     CDS             complement(11617..12957)
                     /gene="gudD"
                     /locus_tag="OO327_002145"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6018683.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12978..14318)
                     /locus_tag="OO327_002146"
     CDS             complement(12978..14318)
                     /locus_tag="OO327_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6018684.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14320..15672)
                     /gene="gudP"
                     /locus_tag="OO327_002147"
     CDS             complement(14320..15672)
                     /gene="gudP"
                     /locus_tag="OO327_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR6018685.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16106..16555)
                     /locus_tag="OO327_002148"
     CDS             complement(16106..16555)
                     /locus_tag="OO327_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR6018686.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16573..17355)
                     /gene="truC"
                     /locus_tag="OO327_002149"
     CDS             complement(16573..17355)
                     /gene="truC"
                     /locus_tag="OO327_002149"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR6018687.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17355..17684)
                     /locus_tag="OO327_002150"
     CDS             complement(17355..17684)
                     /locus_tag="OO327_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR6018688.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18306..18851)
                     /gene="syd"
                     /locus_tag="OO327_002151"
     CDS             complement(18306..18851)
                     /gene="syd"
                     /locus_tag="OO327_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR6018689.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTNLASNLAEFLNQLKPLL"
     gene            18919..19767
                     /gene="queF"
                     /locus_tag="OO327_002152"
     CDS             18919..19767
                     /gene="queF"
                     /locus_tag="OO327_002152"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR6018690.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19879..21243
                     /gene="ppnN"
                     /locus_tag="OO327_002153"
                     /gene_synonym="ygdH"
     CDS             19879..21243
                     /gene="ppnN"
                     /locus_tag="OO327_002153"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR6018691.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHHTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5228 a         5671 c         5067 g         5287 t
ORIGIN      
        1 ttgcagccca accaactatt gtcccacaat tacagcaaca agttacgggt cttacgagta
       61 gcttaaactc acaagaaaag aaagaactga cgcacaagtt agaatctatt ttcaataata
      121 cccaagtgca aattgctgta ttaattgttt ccacaaccaa agatgagaca attgaacaat
      181 atgctacaag agtttttgac aattggcgtt taggagatgc caaacgtaat gatgggatac
      241 tgatcgttgt tgcctagtcg gatcgcactg tccgcatcca ggtaggttat gggctggaag
      301 aaaaggtaac cgatgctctg gctggagata tcatccgtag caacatgata cccgcattta
      361 aacaacaaaa attagctaag ggattagagt tagctataat cgctttgaat aaccaactca
      421 cttcacaaca ccaatatccg gctaatcctt cagaaagtga atcaacgtct tccactgatc
      481 attattactt tgctattttt tgggtatttg cagtgatgtt cttccctttc tggttttttc
      541 atcaaggcag taatttttgt cgcgcatgta aaagtggagt ctgtatttca gcaatccatc
      601 ttttagattt attcctgttc tccgataaaa ctttttccat tgctgtattt tccttctttt
      661 ttactttcac catatttatg gtctttacct gtttatgtgt acttcagaaa agagcatctg
      721 gtagaagtta tcattcagac aacagcggtt ccgctggagg ctcaagtggc ggcgcatccg
      781 gccgctggta actccaccat aaaaaatgcc agcccgaagg ctggcattat caaatcagat
      841 aaagtcagac ttatgcctgg cctttgatct ctttacgacc gttgtacggt gctttttcgc
      901 ccagagcttc ttcgatacga atcagctggt tgtatttagc aacacggtca gaacggctca
      961 tagaaccagt tttgatctgg cctgcagcag taccaacagc cagatcagcg atggtagcat
     1021 cttcagtttc accagaacgg tgagagataa ctgcagtgta gccagcatct ttcgccatct
     1081 tgattgcagc cagagtttcg gtcagagaac cgatctggtt gaatttgatc aggatggagt
     1141 tagcgatacc tttttcgata ccttctttca ggatcttggt gttggttacg aacaggtcgt
     1201 caccaaccag ctggattttg tcgcccagaa ctttggtctg gtatgcgaaa ccgtcccagt
     1261 cagattcgtc cagaccgtct tcgatagaaa cgatcgggta ctgtttggtc agttcttcca
     1321 ggaagtgagt gaattcttca gaggtgaacg ctttgttgcc ttcgccagcc agaacgtatt
     1381 taccatcttt gtagaattca gaagctgcgc agtccatcgc cagagtgatg tctttgccca
     1441 gttcataacc agcagcttta acagcttcag cgataacagc cagagcttca gcgttggaac
     1501 ccaggttcgg cgcatagcca ccttcgtcac caacagcagt gttcatgcct ttcgctttca
     1561 gaacttttgc caggtgatgg aaaacttcag aacccatgcg gatggcttct ttcacagttt
     1621 tcgcgccaac cggctgaatc atgaattcct ggatatcaac gttgttgtca gcgtgctcac
     1681 caccgttgat gatgttcatc atcggaaccg gcatggagta tttgcccgga gtgccgttca
     1741 gttcagcgat gtgctcgtac agcggcatac ctttagcagc tgcagcagct ttggcgttag
     1801 ccagagatac agccaggatt gcgttcgcgc cgaatttgga tttgttttcg gtgccgtcca
     1861 ggtcgatcat gatcttgtca atgccagcct gatctttagc atctttgcca atcagcgcct
     1921 gagcgatcgg gccgtttacc gcagcaacag ctttggttac gcctttaccc aggaaacggg
     1981 atttgtcgcc atcgcgcagt tccagagctt cacgggaacc agtagaagca cctgacggag
     2041 cagctgccat accgacgaaa ccaccctcca gatgtacttc ggcttcaaca gtcgggttac
     2101 cacgggagtc gatgatttca cgaccgatga tttttacgat tttggacatt aggttttcct
     2161 caagtcacta gttaaactga aactccagac aaacaacgcg tacccaaggt acgcgttgcc
     2221 gttctaactt ttttacttac ttcgcctgac gtttctggaa ctcgctggcg gctttcacaa
     2281 agcctgcaaa cagcgggtga ccatcacgtg gagtagaagt aaactccgga tggaactggc
     2341 aagccacgaa ccacgggtga ttcggaactt cgatgatctc gaccaactga tcatccccgg
     2401 aacggcccgc aacgcgcaga cctgcatctt caatctgttt caacagcatg ttgttgactt
     2461 cgtaacggtg acgatgacgc tcaacaattg tcggcgcatt gtatagctgg cgaaccaggc
     2521 tatcgtcaac caactggcac tgctgtgcgc cgagacgcat ggtaccgccg agatcgctct
     2581 tctcgctacg aacttcaacg ttgccgtttt catcgcgcca ctcggtaatc agcgccacaa
     2641 ccgggtactt acagtctggc acaaattctg tagagttggc gttctccatg ttggcaacat
     2701 ggcgagcgta atcaattaac gccacctgca tacccaggca aatgcccaga taaggaatat
     2761 tgttctcacg cgcaaaacgc gcggtcgtaa tcatgccttc tacgccacga tagccgaaac
     2821 cgccaggtac gaggattgcg tccagacctt taaggatttc aacgccgcgc gtttcaacat
     2881 cttgtgaatc gatcagtttg atgttgacgc tgacacgatt cttcagccca ccgtgtttca
     2941 gtgcttcgat cactgattta taagcatccg gcagttcaat gtacttgccg accataccga
     3001 tggtgacttc acttaccgga ttcgcttctt cgaagataac ctgttcccat tcggacagat
     3061 ttgcttccgg gcagtttaag ctgaatcgtt tacaaatata atcgtccagc ccctgagatt
     3121 tcaacaggcc cggaatttta tagatggaat cgacgtcttt cagagaaata accgcttttt
     3181 ccggaacatt acagaacaat gcaatcttcg cacgttcgtt cgccggtaca gcgcgatctg
     3241 aacgacaaat caggatgtca ggctggatac cgatggagag cagctctttt acagagtgct
     3301 gagtcggttt ggttttgact tcaccagacg ctgccatgta cggcaccagc gtcaggtgca
     3361 taaacagagt gtgctcacgg ccaatttcaa cagccatctg gcgaatagct tcgaggaacg
     3421 gcaaggattc gatatcacct actgtaccgc cgatttctac cagtactacg tcatgacctt
     3481 cgccaccttc cagcacgcgc tctttgattg cgttagtgat gtgcggaata acctgcacgg
     3541 ttgcgccgag gtagtcaccg cggcgttctt tacgcagaac gtcagagtag atacgacccg
     3601 tggtgaagtt gttgcggcgg ctcattttgg tacgaatgaa acgctcgtag tgccccaggt
     3661 ccaagtcggt ttcagcgccg tcttcagtaa cgaacacttc cccgtgttgg attgggctca
     3721 tagtacctgg atcgacgttg atgtaaggat ccagtttcat gatggtcaca ttgaggccac
     3781 gggcttcaag aatggctgcg agggaggctg cggcaatgcc tttacccaga gaggatacga
     3841 ccccgccggt cacaaaaata tagttcgttg tcatgctgaa cctgagaagt taggttgaaa
     3901 agacgatgga ataaccaaga cgggaaagca gtatacccga acatgacctg tgccacaaac
     3961 tttcattatc cctcctcttc gccagcgcac tattgaaatc aggagtgaga aaatagcccc
     4021 tttggggtaa atgtttttga cgcaaatcaa gcgcttgtca tttaaaaaat gacacaaatg
     4081 gcgcttgacc gcgtaattcc cttagagatc aatttcctgc cgttttacct gttgccagac
     4141 ttcttccatt gtttcgaggt caacacctgt catttccagt ccacgcgcgg caacaatacg
     4201 ctccacttcg cgaaaacgac gctcgaattt ttcgttcact ttttgcaatg cgatttctgc
     4261 tttcgtccct aaatggcgag ccagattaac cgtggcaaac agcaggtccc ccatttcctc
     4321 ctccagttta gcctggtcga caacagcctg ccgcgcctcg tacatcacct cgtcgatctc
     4381 ttcgtagact ttatcaacca ccgggccaag cgtcgtccag tcgaagccaa cgttggcgca
     4441 acgtttctgg attttttgcg cccgcattaa agccggtaaa ctacgaggaa tatcgtccag
     4501 cgccgaatgc tgcgctttct gcgcgcgctc ttcggttttg atttgctccc aacgggcaag
     4561 cacttcacta ctgttttcgg cagaactatc agcaaaaaca tgcggatggc gacgctctaa
     4621 tttatcgcta atagcagcgc aaatatcatt aaagtcaaag cgcccttctt cctgagccat
     4681 ttgcgcgtaa aacaccacct ggaatagcag atcgcccagt tcaccgcgaa gatcgtcaaa
     4741 atcttcacgg gcgatggcgt ccagcacttc gtaggtttct tcaagggtgt aaggcgcaat
     4801 ggtggcaaat gtctgctctt tatcccacgg gcagccgttt tccggatcgc gcaggcgctg
     4861 cataatagtg agcaaacggt cgatttgatt cattgaattg tcctgaaaat tgcgggtctg
     4921 tcaggtgtaa taccttcgag taaaagatat cgctggatac ggctgacgcc atatccagct
     4981 atgtaagact aactcccgtg caaccgacgc gcgtcgataa catccggcac ctggttgagt
     5041 ttacccagca cgcgccccag cacttgcagg ttgtaaatct caatggtcat gtcgatggtc
     5101 gccagttgct gtttggtgtc gctacggctg gcaacgccaa gcacgttcac cttctcgttg
     5161 gcgagaatgg tcgtgatatc acgtaacaac ccactacgat cattagccac cacgcggacc
     5221 accagcgaat atccggcgga gtagctctca ccccataccg cgtcaacaat gcgttctggc
     5281 gcatgggagc gcagttccgc cagttgttcg caatcggcgc ggtgtactga aataccgcgt
     5341 ccctgggtaa tgaagccgac aatctcatct ccaggaatcg gctggcagca gcgcgcgatg
     5401 tggtgcatca ggttaccaac accttcaacc accacacgac cgttatcttt actgcggttt
     5461 tgcggtgtgt agcttttttg ctgaagctgt ttcagcgcgg cggcgtcctg ctcttcggca
     5521 ctcggcttat taaattgcga ttgcaggaag ttcaccatct gattgagacg gatatccccg
     5581 ccaccaatcg ccgccagcaa ctcgtcgaca tcattgaagt tgtaacgcgg cagcagatgt
     5641 ttttctgctt ctttcaggct gatccccaga tgttccagct cgtcgtcaag gatttgccgc
     5701 ccagccagaa tgtttttgtc acggtcctgt ttacggaacc aggcgtgaat tttcgaacgc
     5761 ccacggctgg ttgtgacgta accgaggttt gggtttaacc agtcacggct ggggttcggc
     5821 tgtttctggg tgataatttc aatctggtcg cccatttgca gctggtaggt gaacggcaca
     5881 atgcgcccgc caatttttgc cccgatgcag cggtgtccga catcactgtg gatgtggtaa
     5941 gcgaagtcca gcggcgttga tcccgcaggc aaatcaacga catcaccttt cggcgtaaag
     6001 acatacaccc ggtcgtcaaa cacctgacta cgtacttcgt cgagcatttc gccggaatca
     6061 gccatctctt cctgccacgc aatcagttta cgcagccagg caatccggtc ttcatgtccc
     6121 gaacgtgcgc cgccagctgc cgcgccctct ttatatttcc agtgcgcagc aacacccaac
     6181 tccgcatctt catgcatctg tttggtgcgg atttggatct caacggtttt tccacccggc
     6241 cccagaacca cggtatgaat agactgataa ccgtttggtt tcgggttagc gacgtaatcg
     6301 tcaaactcat ccggcaggtg gcgatagtga gtgtgcacta tccccagtgc ggcataacaa
     6361 tcctgtaaac gctcggcgac aatacgtacc gcacgcacat caaacagctc atcgaaggcg
     6421 aggttctttt tctgcatttt gcgccagatg ctgtagatgt gtttcggacg accatacact
     6481 tcagctttaa cgccttcagc tttcatctca gcgcgcagat ggccgacaaa ctcttcgata
     6541 tagtgttcgc ggtcgagacg gcgttcatgc agcagttttg cgatgcgttt gtattcggtc
     6601 gggtggagat aacggaagca gtaatcttcc agctcccatt tcagttgccc aatcccaaga
     6661 cggttcgcca acggcgcgta gatattggtg cactcttttg ccgccagtac acgttcatct
     6721 tccggcgcat cttttacttc gcgcagatga gcaatacgct ccgccagttt gatgactacg
     6781 cagcgaaaat catcgaccat cgccaataac atccggcgaa cgttatcgac ctgttcggag
     6841 gaaacagaat cagtgtgcgt cgctttcagc tggcggatcg ccgccatatc acgcacgccg
     6901 tgaataaggt taacaaccga cttaccgacg ctctcacgca gcacatcttc gctgactacg
     6961 ttggcatcag ccagagggaa cagcagcgcc gcccgcagcg tgtcaatgtc catacttaat
     7021 gtcgagagga tctccaccat ctcaacacca cgccacaata acagactggc atccggatgc
     7081 ccctgcgtct gttgcagaca atacgcccag gtttcggcta agcactcaca cgacttctgg
     7141 ctggtaatac ccagacttgc gatccatttt tccggatcaa attcaccagc cttattgata
     7201 tgtgcacttc ttaccgcaac catcgtcctc tcctttaggg accagacctg ccgaaatcgg
     7261 caaatcgtaa ctatttaacg cgcgagaaaa gtaccatcga ttccagatgt cccgtgtgtg
     7321 ggaacatatc cagcatcgcc agtcgcgcaa tggtatatcc tgcttttaat aacgcttcgc
     7381 tatcccgagc cagcgttgca gggttacagg atacataaac tatacgaata ggttccagtt
     7441 ttataatttg ctgcataaca cctgcggcac ctgctcgcgc cgggtccagc aacactttat
     7501 cgaagccgtt tttcgcccac ggctgctttg tgacatcttc ttcaagattt tcgtgataaa
     7561 acgtcacatt ctgtaagcca ttaagacgcg cattctgctg gcctttttcc accagcgcag
     7621 gaacaccttc tacaccgacc acactggcag cttgtgtcgc caatggcagt gtaaagttgc
     7681 ccataccgca gaacagatcc agtacgcgat cttcaggttg cacatccagc cattccaacg
     7741 cacgcgctac cattttttgg ttcacacccg cattgacctg aataaaatcg cgcgggctaa
     7801 aagttaagcg caacccgttt gagtcatacc agggcatctc accagagacg gtttcgagta
     7861 tctcactatc gggggcgaga tacagatcca ggccttcaga atgcgaaaag cgttccagtt
     7921 tttcgcgatc tgccgaactt agcggtgcgg tatggcgcaa aatcatcagc gtgccgctgg
     7981 ttgcctgtac cagttcaaca tgaccaagat ggcgcatagc ttgtaagctg cccagacatg
     8041 ccctgacttt gggcagcaat gcttcaagtt ggggcgctaa aatggggcat tgtttgacgt
     8101 cgacaatgtc actggagccc gctttgcgaa accccatctg aagttgctgt gttttcggta
     8161 agtagttcag acttaaacgc gcgcggcggc gatagcccca gggaacatcg gcgatcactt
     8221 cagagacatc gtgtttcatt aatcgggcga gtgccgcact tttgcttcgc tgctgtaaat
     8281 ccacgctggc gtgttgttgc tgacagccgc cgcatacgcc aaaatgagga cagcgtggcg
     8341 tttcgcgttc cgggctatcg cttaaccggc gtacgacttt agcgcgggca tactgttttt
     8401 tatcttcagt aacagcaact tccgcgtttt cctgcggcaa taatccgggg ataaataacg
     8461 ttttgccgtt atgtcgcgcc acgccctgac caaaagagtc gaggtcgttg actgaaacgg
     8521 ttatgatctg acgcgtcgtc gtgcgtcgtt ttgcagagta gaattgcgcc attggcgaga
     8581 ctttctcaat ttaacagtgt gaccttaatt gtcccataac ggaactccat gaccaactac
     8641 agcctgcgcg cacgcatgat gattctgatc ctggcaccga ccgtccttat tggtttattg
     8701 ctgagtatct ttttcgtcgt gcatcgctat aacgacttgc agcgtcaact ggaagatgcc
     8761 ggtgctagca ttattgagcc gcttgcagtt tctactgaat atggcatgag cctgcaaaat
     8821 cgcgaatcta tcggtcagtt aataagcgta ctgcatcgtc gccattccga tattgttcgc
     8881 gcgatttcgg tttatgatga aaataaccga ctctttgtca cctccaattt tcatcttgat
     8941 ccctcatcaa tgcagctcgg cagcaacgtg ccgtttcctc gccagctcac tgtcactcgt
     9001 gacggcgata ttatgatcct ccgcacgccg attatttctg agagttactc ccccgacgaa
     9061 tcgcccagta gcgatgccaa aaatagtcaa aatatgttgg gatatattgc gcttgagctg
     9121 gatcttaaat cggttcgctt gcagcaatat aaagagatct ttatttcctg cgtgatgatg
     9181 ctgttttgta tcggtattgc gcttattttt ggctggcgct taatgcgcga tgtaaccggt
     9241 ccgattcgca acatggtgaa taccgtcgac cgcatccgtc gcgggcaact cgacagccgg
     9301 gtggaaggat ttatgctcgg cgagctggat atgctgaaaa acggtatcaa ctcgatggca
     9361 atgtcgctgg ctgcttatca cgaagagatg cagcacaata tcgaccaggc gacgtccgat
     9421 ctgcgtgaaa cgctggagca gatggaaatt cagaacgttg agttggatct ggcgaaaaag
     9481 cgcgcccagg aagcggcgcg tattaaatcc gagtttctgg caaatatgtc acacgagctg
     9541 cgtacaccac tgaatggtgt tattggcttt acccgcctga cgctgaaaac agaattaaca
     9601 ccaacgcagc gcgatcacct gaatacgatt gaacgttcgg caaataattt gctggcaatt
     9661 attaatgatg ttctcgactt ctcgaaactg gaagcaggta agctgattct ggaaagtatt
     9721 ccattcccac tacgcagcac gctggatgaa gtcgttactc tgctggcaca ttcttctcac
     9781 gataaagggt tggaactgac gctcaatatt aaaagcgacg tgcctgataa cgtgatcggc
     9841 gacccactgc gattacagca aatcatcact aacctggtgg ggaatgcaat taaattcacc
     9901 gagaatggca acattgatat tctggtagaa aaacgtgcgc tgagtaatac caaagtgcag
     9961 attgaagtgc agattcggga taccggcatt ggtattcctg aacgcgatca atcgcgctta
    10021 ttccaggcct tccgacaggc tgatgccagt atttcccgcc gtcatggtgg caccgggctg
    10081 gggctggtga ttacacaaaa actggttaat gaaatgggcg gcgatatttc gttccatagc
    10141 cagccgaatc gcggttcaac tttctggttc cacattaatc tcgatctgaa cccgaacatt
    10201 attatcgaag ggccatccat ccagtgcctc gcaggtaagc gcctggccta tgtcgaacca
    10261 aactccgcag cagcgcaatg cacgctggat attttaagtg aaacgccgct ggaagtggtt
    10321 tatagcccaa cgttctccgc gctgcctccc gcgcattacg acatgatgtt gttaggcatc
    10381 gcggtgacct tccgcgagcc gctaacaatg caacatgagc gattagcgaa agcggtatcg
    10441 atgaccgatt tcctgatgct ggcacttcct tgccatgcac aagttaatgc tgaaaaactc
    10501 aagcaagatg gtatcggcgc gtgtctgctg aaaccattaa cacctacgcg cctgttgcct
    10561 gccctgacgg aattttgtca tcacaaacaa aacacgcttt tgcctgtaac cgatgaaagt
    10621 aagctggcaa tgacagtcat ggcggttgat gacaaccccg ctaacctgaa acttatcggc
    10681 gcattgctgg aagatatggt gcaacatgtg gaactttgcg atagcgggca tcaggcagtt
    10741 gaacgggcga aacagatgcc gttcgatttg atcttaatgg atattcaaat gccggacatg
    10801 gatggcattc gggcctgcga actcatccac caactcccgc atcagcgaca aacgccggtt
    10861 atcgcggtaa cggcgcatgc aatggccggg caaaaagaga agctgcttgg cgcagggatg
    10921 agcgattatc tggcgaaacc gattgaagaa gagcgattgc ataatttgtt gttgcgctac
    10981 aagcctggca gcggtatttc gtctcgcgtc gtgacgcccg aagtcaacga aattgtggtg
    11041 aacccgaatg cgaccctcga ctggcaactg gcactacgcc aggcagcagg aaaaaccgat
    11101 ttagcgcgcg atatgctgca aatgttactc gatttcctgc ctgaagttcg caacaaagtt
    11161 gaggaacagc tggctggaga aaacccggaa ggcctggttg atttgattca taaactgcat
    11221 ggcagttgcg gctatagcgg cgtaccacgt atgaagaatc tctgccagct gatcgaacaa
    11281 cagctacgta gcggtactaa agaagaagat ttggagccgg agctgctgga actgttggac
    11341 gagatggata atgtcgcgcg cgaagccagc aaaattctcg ggtaacggac gtcttgtcga
    11401 gtagcaatcg gctttttatg acgctggttt cagattggtg acaaagtgcc tgtgtttatg
    11461 ccggatgcgg cgttaacgcc ttatccggcc tacaaaacca attaaattca atgaattaaa
    11521 aaatgatgta gacctgatac atagcacaac aggctatttt gcgtttagca tcagtctcaa
    11581 accggctcca gatagagccg gttttggttt tctgtcttaa cgcaccatgc acgggcgctt
    11641 gttatcgaac gtccagccag gaatcagata ctgcattccc atcgcatcgt cacgcgcgcc
    11701 aagcccgtgt ttctgataca gctcatgggc tttcatcact tgatccatat cgatttctac
    11761 acccagcccc ggtttttctg gcacctgtac cagcccgcct ttgatctcaa acggttcttt
    11821 ggtcaggcgc tgattacctt cctgccagat ccagtgcgta tcaatagcag taattttacc
    11881 cggtgcagcg gcggcaacat gggtaaacat cgccagggaa atatcgaagt ggttgtttga
    11941 gtgtgaaccc caggtcaggc caaattcatg gcacatttgc gccacacgta ccgaaccttg
    12001 catagtccag aaatgcggat ccgccagcgg gatatcaacg gattgtaacg agagcgtatg
    12061 gcccatctgc cgccagtcgg tggcgatcat attggttgca gtcggcaggc cagtcgcgcg
    12121 acggaactct gccatcactt cacgcccgga gaaaccttgc tccgcaccac acggatcttc
    12181 tgcataagcc agcgaacctt tcaggtattt accaatttta attgcttcgt tcagcgacca
    12241 ggcaccgtta ggatcgagcg taatacgcgc ctgcgggaag cgttgcgcca gtgccacaat
    12301 agactcggct tcttcttccc cggccagtac accgcctttc agtttgaaat cgttgaagcc
    12361 atatttttca tacgccgctt ccgccaggcg caccaccgca tccggcgtca tcgcttcttc
    12421 atgacgcagg cgataccagt cgcatgagtc atccggctgg ctttgatacg gcagaggtgt
    12481 ggctttgcga ttaccgacga agaacagata accgagcatt tcgacttcgc tacgctgttg
    12541 accatcgccc agcagcgatg ccacgtttac ccccagatgc tgccccagca gatccagcat
    12601 tgccgcttct atcccggtaa ctacatgaat agtggtacgc aaatcaaatg tctgcaaacc
    12661 gcgcccacta gcatcacgat cggcaaaagt attacgcacc agcgtcagaa cgtttttgta
    12721 ttcacccagc gttttaccta ccaccagcgg aatcgcatct tccagcgttt tacggatttt
    12781 ctcgccgccg ggaatttccc ctacgccagt gtgaccggaa ttatctttga taatcacaat
    12841 attacgcgtg aagaacggtg cgtgtgcacc actcagattc atcagcatac tgtcatgacc
    12901 cgccaccggg atgacctgca tttcagtaac aacaggcgtc gtaaattgag aactcatatt
    12961 tatgtccttt ttcagaatca atgacggccg aaaacgggac gtttacggtc aaaggtccag
    13021 ccggggatca ggtactgcat cggacctgcg tcgttacgcg caccgcaagg cagacgttta
    13081 taggcctcat gtgccttttg tacctgttcc cagtccagtt ccacgcccag accgggcgca
    13141 tcaggaacgg caatttttcc gtttttaatc tccagcggat ttttggtcag gcgacaatcg
    13201 ccctcctgcc aaatccagtg ggtatcgata gcggtaggat tacccggtgc cgccgcgccc
    13261 acatgggtaa atatcgccag agagatatcg aaatggttgt tggaatggca gccccaggtc
    13321 agcccccagt cgtcgcaaag ctgcgccacg cggactgcac cggaaagcgt ccagaagtgc
    13381 ggatcggcaa gtggaatatc taccgcattg agcatcaccg catgacccat ttcgcgccag
    13441 ttggtggcga tcatgttagt cgcgacgggc aagccggtcg cccgtcgaaa ctccgccatc
    13501 acttcacgtc cggagaagcc ctgttctgcg ccgcatggat cttcggcata ggtaagaaca
    13561 tcattcagcc ctttgcataa agaaatggct tcatcaagca gccatgcacc gttgggatca
    13621 acggtaatcc gcgcatccgg gaagcttttc ttcaatgcac gaacagtgtc gatttcttgc
    13681 tcgccaggta acaccccgcc cttaagtttg aaatctttaa agccatagcg atcctgtgag
    13741 gcttccgcca gacgcacaac ggcttcgctg ttcatcgctt tctgatggcg caactgatac
    13801 cactcatggt tgcccggcgt attttccaga taaggaagat cggttttggt ccgatcacct
    13861 atataaaaca gataaccgag gacggtaata gtctcgcgtt gcttgcctgg ccccaacagt
    13921 tcgcagaccg gaacattcag cgccttacct agcaggtcaa gcaaggcggc ttccagcgcc
    13981 gccacggcat taacgcgcaa ttcaaaagtc caggcacctt tgccaaaggt atcaaaatcg
    14041 gctgcctgat tacctttatg tacctgctga accactttat tcaggcgcgc aacttcctgg
    14101 cccagaacca tcggaatagc atcgaccagt gtttgataaa tcacctctcc gcccggcgct
    14161 tcaccaatgc cggtatgccc ggcgttatcg gtgagtacca cgatattgcg ggtgaagtat
    14221 gcgttatgcg caccaccaat attgagcaac atgctgtcat gtccggcgac cggaatgact
    14281 ttcatatcag taataacagg actggattgt gtcgccatct tattgccccg cgacaggttt
    14341 cagctcgata cgcttgatat cgcccaccag caccaggtag ctcagtaccg cgattagggc
    14401 atgaacacca acataaatca gcgccccatt aaacgagcca gtcgtgccaa cgatataacc
    14461 aattgcgatt ggcgtgacga tgccagaaat gttaccgaac atgttgaaca ggccaccgga
    14521 aagaccgctg atctcttttg gcgcggtatc tgccattact gcccaaccca gcgccccgat
    14581 gcccttaccg aagaaggcca gcgccataaa gccgatgatc atccactcaa cgttgacgta
    14641 gttgcagaac accatcacca tcgacagcaa catgcccatt acgatcggtg ttttacgcgc
    14701 aatgttcagc gatcccgtgc ggcgcatcag ccagtcggaa ataattccac ccagcacacc
    14761 gccgataaaa ccgcaaaccg ccggaacgga agccacaaag cccgctttca gaatcgacat
    14821 cccacgcgcc tgcaccagat aaaccggaaa ccaggtaatg aaaaagtaag tcagggcgtt
    14881 gatacagtac tgaccgatat aaacgccgat catcatccgt gacccgagca gctgtttgat
    14941 ctgcccccac ttcacgctga acggcacttt aactttggtg ctttgctgat ccatattgat
    15001 cagcgcgcca cccgcggcga tatactccag ctctttctga tttacccccg gatgttgatt
    15061 tggctcgtgg atgactttca accagataaa gctgatgaca atgcccagac cgcccataaa
    15121 gaagaagacg tgtgaccagc ccacttcatg cgtcagccag cccataatcg gcgcgaagat
    15181 caccgttgcg aagtattgag cggagttaaa aatcgacacc gccgttcccc tttcctgcac
    15241 cggaaaccag gccgcaacaa tgcgactgtt gccggggaaa gatggcgctt cagcaagccc
    15301 gaccaggaag cgcagagtaa acagggcaac gataatgccg aatccactaa agatatcgac
    15361 gaagccttgc agcaaggtaa acatcgacca gataaagatc gaccagaagt agacgcgttt
    15421 tgaaccaaaa cgatccagca accaaccacc agggatctgc ccgataacat aagcccatga
    15481 gaaggcagag aacacatagc ccattcccac gggatcaagg ccgatatctt tggccatttc
    15541 cgaaccggca atagaaagcg tagcgcggtc gccgtagttg aaggatgtga cgataaacaa
    15601 catcaccact atccagtaac gagcattcgt gcgtttttcc acactgctcg cagcctgact
    15661 taaagaactc attgttgcac tcctgaaaat tcgcgttagc cacgctcact ctggactgcg
    15721 acatcgccag gaaatcagag gtgacgtagg gtgttttttg ccgtttttat aggtcgttcg
    15781 ccgaatacgg cgcgtgttta tatctggcaa tagcagtata aaaagcgcgc catagcggct
    15841 caccgtgcaa caacacaaca ttaatgcgtt caatgaggcc cgattttggc attagccctg
    15901 gacggtggaa tccacttcac ggaaatgaaa acaagaacaa gaaaggaagg gtaaaaacga
    15961 agaaataaag aagagtatga aatggatcgc ttgactctag gcaaacgcca gtaaaaatcc
    16021 gcgctatgaa gcagttttta ctggcatttg cctgaaaaga ttcgattcag caccgctaaa
    16081 acgacattta ccgctcgctg acatatcagg acaacagcgt gccccactgt tcgacccacg
    16141 gatttgattc cgtttccggt tccgggttct cactggcgtc aatcaacagc atttcgccaa
    16201 cccgctgagc gctctgttcc tgcaacaagg catcgaactg tttgccgcca ttgcaaaaat
    16261 ttacataact gctgtcgccg agcgcaatca cgccataacg cagattcggc tggaagccca
    16321 gactatcttt gattccctga aagagcggca caatgctatc aggaaggtcg ccctgcccgg
    16381 tcgtggacgt aaccaccaga acatacttat cctgataggg cagccagtcg cttaattcag
    16441 gatcttcaaa taccgttgct ttgtggccct gtgcggtcag aattgcttct gcttcttccg
    16501 ccactaacag cgaatttccg tacatggtgc cgacaaaaat accaatttcc gccataccgt
    16561 tttctccctg gattaggaac ttatctctcc atcctgaccc gatggcgcac tgaactcaac
    16621 cctttcattt tcaggaagca gaccgcgcca gccaaattgt gataacgcct gcatccaggt
    16681 gtcgtccagg cccgcgtgaa tagtcagcgg ctcgccagta aaaggatgcg tcaatgacaa
    16741 ctgactggca tgtagcatta accgctggag gccaaaatgc tcagcaccgc tgcgattctg
    16801 gcgtaaatcg ccatgtttgc tatcgccaat aatcggatga cgcaaatggg caagatgtcg
    16861 tcgaagctga tgtttgcgtc cggttttcgg ctccagttcc accaggccgt agcgcgtggt
    16921 cgggtaacgt ccggtcgcta ccggcatttc tacggtcgcc agaccgcgat aatgcgtcac
    16981 tgctggctgc gggcctttat cttcgcgggc aaatttatca gcgattttgt ccagttcttc
    17041 caccagtgga taatccaaca ccgcttcttc catcaaccag ccgcgcacaa tcgcatggta
    17101 acgtttctgg atttggtgct gttcaaactg ttgtgccagc agccgtccgg cctcgctgga
    17161 taatcccatc aacaacacac cagaagtggg tcggtccaga cgatgagcag taaaaacatg
    17221 ctgtcctatc tggtcacgca cggtttgcat gaccactact ttctcgtcgc gatccagcca
    17281 gctacggtga accagccagc cggagggttt atttaccgca accagccatt catcctgata
    17341 gagtatttcc agcattagct cgcatcatcc gcaaaaagag catccagttt ttccaactca
    17401 gccagaataa gcgcgcgttg cggatggtcc gtcgccagcg ccatttcata atagggtgca
    17461 acagcaaaag cgcccggtaa cggctggttg ttatttaaca aatcgtgcat tcgcgggatc
    17521 agcccccact gcaaccactc cagtggttcc atggtgtcca taaagaacgg ttgggtacta
    17581 ttaaattgat gcggctgggg ttcatcgttt cgccagtgct gatgttcacg cagtaacgct
    17641 tcaagcgcct gcaactggag acgaacgcgg tcatgagtgg tcataaagca acctcaataa
    17701 gaaaaactgc cgcgaaggat agcaggaata aaaaaaggga gcactgtatt cacagtgctc
    17761 ccggttcgtt tcgcagcatt ccagctactt ttgttgctcc ctgctcatcc ttgacaactt
    17821 ttcctctggc cttgcggcca atcgttcatc ctgaactatt gcttcctgct cacaccaccc
    17881 cgatgtgata cttcatcctg aagtgtccct ggccttcctg acccaccgaa tcatcctgac
    17941 cggttctcat tctccatcct ggaggtgtcc tttaacgcgt cctgcgtcat cctcttcgct
    18001 tcatccagaa gcctttccct gaaacaccat cctggtgtgt cctgcagaag tgtcatcatc
    18061 ctgatgttca cttcgttgtc tgactccctg tcgacgaaga tagaatcgtc tttttcgcga
    18121 agtcttacaa ggcgcttaca gacaatgcct taaggaaaat ttcatatgaa agttaagaat
    18181 taacaatcaa cattttgatt ttaaatgaaa ataacgattc ttatcttttt aagtattcgc
    18241 ttatttcctg gcgatctcct acagagtctg taagagatct ctcacaacca gatggggtat
    18301 ttggattaca gaagaggctt aagttgatta aggaattccg caagatttga ggctaaattc
    18361 gtccgtttac gtgttcccag cgtctctttg cacacttcac cggagagatt acacaccgag
    18421 atgacctcta actcttcttc cagcgtcgcg ataaatagcg tgggcggcag ttttagcctt
    18481 ttctgagtca ccagatgacc aatcagattt tcctgcaccc gacggaagtc gtcttcactc
    18541 caggtttgca gcaatgtcag tttgatatcg ccaaactgcg cgtgcatatc cccggcaaac
    18601 tgagtcgtat aaaaggtatg aattgtgggt tgtatcacaa tatcaaaagc gcgttcaacc
    18661 gcgtttacat tttgttcccc ggtaaacggc tggggttgcc agtatacggc atcttcggta
    18721 gttgaaataa tgcacggtga aggaacaccg tacaattcct cacttaacgg ccagctttta
    18781 tgctcttcat gccacgcatc gcagtagcgt gcagtaaaat ctttcagggc ttgtgcggtc
    18841 aaatcgtcca ctaatttctc tcttcactta aaccagatac acttgtcgtt tagtttatct
    18901 ggtttatgac ggtgaaacat gtcttcttat gcaaaccatc aggcacttgc gggcctgact
    18961 cttggaaaat caaccgatta ccgggatacc tatgacgcca gcctactgca aggcgttcca
    19021 cgcagcctga atcgcgaccc gctgggtctg aaagcggata acctgccttt tcacggtacg
    19081 gatatctgga cgctgtatga actttcctgg ctgaatgcga aaggtttgcc gcaggtcgct
    19141 gtcggtcatg ttgaacttga ttacaccagc gtaaatctga ttgagtcgaa gagttttaag
    19201 ctctatctca acagttttaa ccagacgcgt tttaataact gggatgaggt gcgccagacg
    19261 ctggagcgcg acttaagcac ttgcgctcag ggtaagatta gcgtggcgtt atatcgtctt
    19321 gatgaactgg aaggccagcc gataggtcat ttcaatggca cttgcattga tgaccaggat
    19381 atcactatcg ataactatga attcactact gactatctgg agaatgccac cagcggtgaa
    19441 aaagtagtgg aagagacgct cgtcagccac ctgctgaaat caaactgcct gatcacccat
    19501 caaccagatt ggggttcgat ccaaattcag tatcgtggac gccaaattga cagagaaaaa
    19561 ctgctgcgtt acctggtctc attccgtcat cacaacgagt tccacgaaca gtgcgtggaa
    19621 cgcatcttta atgacctgtt acgcttctgc cagccagaga aattgagcgt ttacgcacgt
    19681 tatacccgtc gtggcggtct ggacattaac ccgtggcgca gtaatagcga ttttgtccca
    19741 tcgaccacaa gactggttcg gcaataaatt ttttctcaat tttgcgtgct ggattcacgc
    19801 agaaggttgt gaaaggtcat caggcagggc tattgtaatc aaagggaatg acgatattcg
    19861 tcccataagg agtttttctt gattacacat attagcccgc ttggctccat ggatatgttg
    19921 tcgcagctgg aagtggatat gcttaaacgc accgccagca gcgacctcta tcaactgttt
    19981 cgcaactgtt cacttgccgt actgaactcc ggtagtttga ccgataacag caaagaattg
    20041 ctgtctcgtt ttgaaaattt cgatattaac gtcctgcgcc gtgaacgcgg cgtaaagctg
    20101 gaactgatta atcccccgga agaggctttt gtcgatgggc gaattattcg cgctttgcag
    20161 gccaacttgt tcgcggttct gcgagacatt ctcttcgttt acgggcaaat ccatcatacc
    20221 gttcgttttc ccaacctgaa tctcgacaac tccgtccaca tcactaacct ggtcttttcc
    20281 atcttgcgta acgctcgcgc gctgcatgtg ggtgaagcgc caaatatggt ggtctgctgg
    20341 ggcggtcact caattaacga aaacgagtat ttgtatgccc gtcgcgtcgg gaaccagctg
    20401 ggcctgcgtg agctgaatat ctgcaccggc tgtggtccag gagcgatgga agcgccgatg
    20461 aaaggtgctg cggtcggaca cgcgcagcag cgttacaaag acagtcgttt tattggtatg
    20521 acagagccgt cgattatcgc cgctgaaccg cctaacccac tggtcaacga attgatcatc
    20581 atgccggata tcgaaaaacg tctggaagcg tttgtccgta tcgctcacgg catcattatc
    20641 ttccctggcg gtgtgggtac ggcagaagag ttgctgtatt tgctgggaat tttaatgaac
    20701 ccggccaaca aagatcaggt tttaccattg atcctcaccg gcccgaaaga gagcgccgac
    20761 tacttccgcg tactggacga gtttgtcgta catacgctgg gcgaaaacgc gcgccgccat
    20821 taccgcatca tcattgatga cgccgctgaa gtcgctcgtc agatgaaaaa atcgatgccg
    20881 ctggtgaaag aaaatcgccg tgatacaggc gatgcctaca gctttaactg gtcaatgcgc
    20941 attgcgccag atttgcaaat gccgtttgag ccgtctcacg agaatatggc taatctgaag
    21001 ctttacccgg atcaacctgt tgaagtgctg gctgccgacc tgcgccgtgc gttctccggt
    21061 attgtggcgg gtaacgtaaa agaagtcggt attcgcgcca ttgaagagtt tggtccttac
    21121 aaaatcaacg gcgataaaga gattatgcgt cgtatggacg acctgctaca gggttttgtt
    21181 gcccagcatc gtatgaagtt gccaggctca gcctacatcc cttgctacga aatctgcacg
    21241 taatctcccc tcc
//
LOCUS       DAJTSI010000065        21251 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000065 DAJTSI010000000
VERSION     DAJTSI010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21251
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.065"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1853)
                     /gene="glyS"
                     /locus_tag="OO327_002154"
     CDS             complement(<1..1853)
                     /gene="glyS"
                     /locus_tag="OO327_002154"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR6018692.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQV"
     gene            complement(1863..2774)
                     /gene="glyQ"
                     /locus_tag="OO327_002155"
     CDS             complement(1863..2774)
                     /gene="glyQ"
                     /locus_tag="OO327_002155"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR6018693.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(2869..3168)
                     /locus_tag="OO327_002156"
     CDS             complement(2869..3168)
                     /locus_tag="OO327_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR6018694.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3343..4338
                     /gene="wecH"
                     /locus_tag="OO327_002157"
     CDS             3343..4338
                     /gene="wecH"
                     /locus_tag="OO327_002157"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR6018695.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4380..4817)
                     /locus_tag="OO327_002158"
     CDS             complement(4380..4817)
                     /locus_tag="OO327_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCR6018696.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(4863..5204)
                     /locus_tag="OO327_002159"
     CDS             complement(4863..5204)
                     /locus_tag="OO327_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018697.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5373..6827)
                     /gene="xylB"
                     /locus_tag="OO327_002160"
     CDS             complement(5373..6827)
                     /gene="xylB"
                     /locus_tag="OO327_002160"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR6018698.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(6899..8221)
                     /gene="xylA"
                     /locus_tag="OO327_002161"
                     /pseudo
     CDS             complement(6899..8221)
                     /gene="xylA"
                     /locus_tag="OO327_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8587..9579
                     /gene="xylF"
                     /locus_tag="OO327_002162"
     CDS             8587..9579
                     /gene="xylF"
                     /locus_tag="OO327_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6018699.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9657..11198
                     /gene="xylG"
                     /locus_tag="OO327_002163"
     CDS             9657..11198
                     /gene="xylG"
                     /locus_tag="OO327_002163"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCR6018700.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     ITQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11176..12357
                     /gene="xylH"
                     /locus_tag="OO327_002164"
     CDS             11176..12357
                     /gene="xylH"
                     /locus_tag="OO327_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCR6018701.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12435..12965
                     /locus_tag="OO327_002165"
     CDS             12435..12965
                     /locus_tag="OO327_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR6018702.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLAD"
     gene            13077..13613
                     /locus_tag="OO327_002166"
     CDS             13077..13613
                     /locus_tag="OO327_002166"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR6018703.1"
                     /translation="MIGIDNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(13721..14545)
                     /locus_tag="OO327_002167"
     CDS             complement(13721..14545)
                     /locus_tag="OO327_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCR6018704.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            14865..16895
                     /gene="malS"
                     /locus_tag="OO327_002168"
     CDS             14865..16895
                     /gene="malS"
                     /locus_tag="OO327_002168"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR6018705.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17073..18326
                     /gene="avtA"
                     /locus_tag="OO327_002169"
     CDS             17073..18326
                     /gene="avtA"
                     /locus_tag="OO327_002169"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCR6018706.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18477..18950)
                     /locus_tag="OO327_002170"
     CDS             complement(18477..18950)
                     /locus_tag="OO327_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR6018707.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19052..19900)
                     /gene="yiaJ"
                     /locus_tag="OO327_002171"
     CDS             complement(19052..19900)
                     /gene="yiaJ"
                     /locus_tag="OO327_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCR6018708.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20100..21098
                     /gene="yiaK"
                     /locus_tag="OO327_002172"
     CDS             20100..21098
                     /gene="yiaK"
                     /locus_tag="OO327_002172"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCR6018709.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21110..>21251
                     /locus_tag="OO327_002173"
     CDS             21110..>21251
                     /locus_tag="OO327_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR6018710.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYA"
BASE COUNT         5336 a         5414 c         5222 g         5279 t
ORIGIN      
        1 acctgcatcg ccagtttaat ttcttccggc tctttcaggg tagaggcatt cacgcgatcg
       61 ctcagcactt cgtcagattt cgccaggatg ttagatacac gtttgttcgc cgccgccagt
      121 gctgcagctg cttccagggt acggaagtgc gataccgctt tcatgcgggc atcgaaatca
      181 gccggacgag tcggacgacg cgccagtacc gcctggatgg tgtcaacggt ataaccttcg
      241 tcctgatacc aggcgcggaa gcgaccgagc ataaagtcga taacatcatc aactacgttg
      301 gcattagtca gcttatcgcc atacagacgc accgcttctt cggtcagcgt ttgcagatca
      361 aggttgaggt tcttctcaac gataattcgc agcacgccaa gtgcggcacg acgcagcgca
      421 aacgggtctt tgtcgccttt cggatgctga ccgataccga agatacccgc cagagtatcc
      481 atcttatcag caatcgccag cgcacaagct accgggttgg acggcaggtc atcaccggca
      541 aagcgcggct gatactgctc attcagcgcg acggctacgt cttccgcttc gccatcgtga
      601 cgcgcgtagt gcatccccat aacgccttgg gtgtcggtga actcgaagac catgttggtc
      661 atcaggtcac acttagacag caggcccgca cgggttgcgt ggttaacgtc agcgccaatc
      721 tgttcggcga tccagccagc cagcgcctgg atgcggtcag tcttgtcacg cagcgtaccc
      781 agctgttgct ggaacagcac ggtttgcagg cgcggcaggt tatcttccag acgttttttg
      841 cggtcggtgt tgaagaagaa ctcggcatcc gccagacgtg gacgaacgac tttctcgtta
      901 ccagagataa tttgctgcgg atctttcgat tcgatgttgg caacgaagat aaagttcggc
      961 agcagtttgc cgtcgttcgc atacaccggg aagtacttct ggtcaccttt catggtgtaa
     1021 accagggctt cagccggcac cgcgaggaat ttctcttcga attttgcggt cagaacgacc
     1081 ggccactcca ccagcgaagc cacttcttcc agcaggcttt cgcttaagtc agcgttaccg
     1141 ccaatcttac gcgctgcttc ttcggcatcg gctttaatct tcgctttacg ttcttcgtaa
     1201 tcggcgatga ctttcccgcg ctcacgcaga atttccggat actgatcggc gttgtcgatg
     1261 gtgaattccg gttcgcccat aaagcggtgg ccgcgaatca cgcgatcgga ctgaatgccc
     1321 agaatggttg ccggaatgac tttgtcgccc agcagcaggg tcacggtgtg caccggacgc
     1381 acgaagtgca cgtcgcttgc accccagcgc atcagtttcg ggatcggcag tttcgccagc
     1441 gaagtcgcaa ccatattcgg cagcagtgct tcggtgcttt cgcccttcac atgggcgcga
     1501 tacagcagcc attcgccttt atcggtagtc agacgctcag cctggtcaac ggtaatacca
     1561 caaccacgcg cccaaccttc tgccgctttg ctcggttttc cttcagcgtc gaacgcctgg
     1621 gcaatcgccg ggccgcgttt ttcgatttca cgatctggtt gtgcttccgc caggttagct
     1681 actttcagcg ccagacgacg cggagcagca aaccattgaa cggtgccgtg tgcgaggcct
     1741 gcgttatcca gctccgcagt aaagttcgca gcaaaggact ctgccaggct gcgcagtgct
     1801 tttggtggca gctcttcagt gccgatttcc accagaaaag ttttctcaga catagccgcc
     1861 tcttacttat ctttgttgca catcgggaag ccgagggctt cacgggaagc gtaatatgct
     1921 tctgccactg ctttggtcag ggtgcgaatg cgcagaatgt agcgctgacg ctcggtgacg
     1981 gagatggctt tacgcgcatc cagcaggttg aagctgtggg cggctttcag aatacgctcg
     2041 taggctggca gcggcagcgg attttccagc gccagcagct gctgcgcttc tttctcgtac
     2101 tgctcaaagc aggtgaacag gaagtccaca tccgcgtatt cgaagttgta agtggactgc
     2161 tccacttcgt tctgatggaa cacgtcgccg taggtggttt tacccagcgg gccgtcgctc
     2221 cagactaggt cgtaaacgct gtctacgccc tgaatgtaca tggccagacg ttccagaccg
     2281 taggtgatct cgccggtaac cggtttacac tccagaccac caacctgctg gaagtaagtg
     2341 aactgcgtca cttccatgcc gtttagccac acttcccagc ccagtcccca ggcccccaga
     2401 gtcgggtttt cccagttatc ttccacgaaa cggatgtcgt gaatagtcgg gtccatgccc
     2461 agctctttca gagaaccgag gtacagctcc tgaatattgt ccggcgatgg cttaatgacc
     2521 acctggaact gatagtagtg ctgtaaacgg ttggggtttt cgccgtagcg accatcggtc
     2581 gggcgacgag aaggctgaac ataagcagcc gccatcggtt ctggccccag cgcgcgcaga
     2641 caggtcattg ggtgagaggt tcccgcgccg acttccatgt ccaatggttg aacaatggtg
     2701 cagccctggc gagcccagta atcctgtaag gtcaggatca agccctggaa ggtcctggta
     2761 tcaaactttt gcatattatt tcgtgctgga tacgtgtgga tttaaaggaa gggatcagta
     2821 tacccgctgg atggaagata tacagtacga aacgggaaaa agcagggctt aacgcatgga
     2881 aagatgcaaa aaatggccat ctgcgtcaaa agaacagaca aactgctctt tacggaaggt
     2941 ataaccgcgc atttcatagc ttccctggaa ctgttcgaag gcggtgacgt cgattttttg
     3001 ctcgccggtg ttataacgtt ttgccgcctg atccttgcat aacgcttcca tattcaacga
     3061 acgcagagga tcaaccctta cccgttgtgc tttctgcacg ggtgacggtg tagaacaacc
     3121 cactagcacc ataagcgcca ttgccggaaa gaaagcgttc atcatcatta aattaccgtc
     3181 tggtttgcag gtgagtctta ttatttatat cggtatagtg gctgatatct tacgaatttc
     3241 cttaagcctc tataggctgt agtgataaaa ctcagattat tccagtgcgc caaccaaatg
     3301 atttatcggt cagacttttt ttaaccagat acagagacac agatgcagcc caaaatttac
     3361 tggattgata acctgcgagg gatagcgtgt ttaatggtgg tgatgattca caccactacc
     3421 tggtatgtga ccaatgctca tagtgttagc cccgtcacat gggatatcgc caatgttctg
     3481 aattctgcct ctcgtgtcag cgtgccgcta tttttcatga tttccggcta tctctttttt
     3541 ggcgaacgca gcgcccagcc gcgccatttc ttgcgtatcg gcttatgtct gattttttat
     3601 agcgcaatcg cactgctcta cattgcgctg tttacctcca tcaatatgga gttagcgctg
     3661 aaaaacctgc tgcaaaagcc agtgttttac cacttgtggt ttttcttcgc gattgcggtg
     3721 atttatctgg tttcaccgct gattcaggtg aagaacgtcg gcggaaaaat gttgctggtg
     3781 ctaatggtgg tgattggcat tatcgctaac ccaaacacag tgccgcagaa aattgacggt
     3841 tttgaatggc tgccaattaa cttatatatc aatggcgata ctttttacta cattctgtat
     3901 ggcatgttgg gccgcgctat agggatgatg gacacacagc ataaagcact gtcgtgggtg
     3961 agcgccgcgc tgtttgcgac gggggttttt attatctctc gcgggacatt atatgaattg
     4021 cagtggcgcg gaaattttgc cgatacctgg tatctttact gtgggccgat ggtttttatc
     4081 tgcgcaatcg cgctattgac tctggttaaa aacacgctgg atacgcgtac cattcgcgga
     4141 cttggcttaa tctcccgcca ttcattgggt atatacggat tccacgcctt gattatccat
     4201 gcgctgcgca cccggggaat tgagcttaaa aattggccaa tactggatat tatttggatc
     4261 ttttgcgcga cgttggcagc gagtttgtta ctttctatgc tggtacaacg aatcgacaga
     4321 aacagattag tgagttaagt aaaagcccgt tcacattgga ctgaccgggc ttacgtgaat
     4381 tattcgctgt attcttcctg ggtaacctgt gtttcttttg gcgggtttat tccggcatca
     4441 cgaatattct tctgcaccgc tactgcacca aacaagctta agaagaaagc cagtccataa
     4501 aaaccttttt cactgagtaa taatgtcgca ttccacagac ctaccatcag tagagcaaca
     4561 gagataataa agacagtcag gcaggtcata taataaatgg aagtggtcgg tatgccttca
     4621 tacttgtccc gaacggtctt ttgataagac gcagcagaaa acagtcctaa caccagtacg
     4681 gcaaaataat accctttttc atttaactgc atctctgcat tccatagccc taacaggtag
     4741 gtaacgatac caccaacgag agctatccat gacacaatac taaaggccgg tgaataggtt
     4801 gatattttgt tgtccatcac agtattcctt tcattcctga atgtgtaaga gctttatgtt
     4861 gctcataccg agcgaaacaa acgcatttga ccaaacaagc agacaaaaaa ggcgatgggg
     4921 tagatcacca tttcataagc attgataggt gcatttaaaa caccgataaa caagagaccg
     4981 atagtgatta acgcaactaa ctggcagata tgggtaaaaa aattatccag ttgcccgagt
     5041 ttttctgcgc gaagatatgc atagttacca aaagttgctg tcattaacac gccaagaaaa
     5101 tagccttttc cacttagcaa tgggcatgag atccatagcc caaccagata aaccagcgcc
     5161 ccgacaacaa ataatagttt tgccacagtt tttgaggttt tcataacgat ctccatatct
     5221 accagcgata cattacgagt aaccaacgaa agacaaaact gaaaaatgcc attaacaaat
     5281 gattttcaga ataaattcat actaaatatt aattaattag tgagatatat agatgtgaat
     5341 tatcccccac ccggtcaggc aggggataac gtttacgcca ttaatggcag aagttgctga
     5401 tagaggcgac ggaacgtttc acgtcgtggc tgataagcgg cataacgctg cgcatctggt
     5461 agatgcgact gttctaacgg tagctgcggc aacaactcaa tgagcgattt ctctggattc
     5521 gccgcaatct gcgccagcct tgctgcgccc agtgctggcc ccacatcccc ccccgtacgg
     5581 taatcgagct gctgaccgct gatatccgcc agcatctgac gccagtactc actacgcgcc
     5641 ccgccaccaa tcaacgtaac actttgcggt ttaatgccgc aggcatgcac gacatccatg
     5701 ccatctgcca gcgcataacc cacgccttcc agcactgctc gcgccagttc attggggcca
     5761 tgttgatgag tcaaaccaaa gaaaaccccc ttcgcctggg gattattgtg tggtgtacgc
     5821 tcgccggaaa gataaggcag aaaccaaact ggctcggcac tttcatcagc ctgttgagct
     5881 gcagcgatta aagctgggac attgcacagg ccggttaatt tcgcggccca atccagacac
     5941 gatgctgcac tcagcattac agacattaaa tgccaacgct gcggtagcgc atggcaaaag
     6001 ctgtgtacag cgctttctgg cttgcttaag aacccttcgc tgacagcaaa atagaccccc
     6061 gacgtcccca gcgataacat tgcctgatta gcatcaacca ttcccacacc aactgcacca
     6121 gctgcattgt cgccaccgcc tgcgacaact ggcaccgtcg ccatacccca cgctttcgca
     6181 acttcaggta acaaagcacc agtaatttcg ctgccttcgt ataatgcggg catctggtca
     6241 cgagataagt cgcaagcctg cagcatgacg tcactccagt cacgctttgc gacatccagc
     6301 cacatggtgc cagctgcgtc agacatatcg ctggcaaact cccccgtcat acgcagacgc
     6361 aagtaatctt tcggtaataa tactttgtcg atttgacgga atatctccgg ctcatgccgc
     6421 tgaacccata gcaatttagg cgcagtaaat ccgggcatca tcaggttgcc ggtaatcact
     6481 cgtgattgcg gaactctcgc ttccagcaaa gtgcactctt gcgcacagcg cccgtcgttc
     6541 cacaaaatgg cagggcgcaa tacccgttgt tgagcatcca gtaaggttgc tccatgcatc
     6601 tggccggcaa tacccaatgc tttaacgtcc tgcagagaat gctgatcgcc cagagctttc
     6661 attgcgcgat cagttgcctg ccaccactgt tccgggtctt gttccgacca aagtggatgc
     6721 gggcgcgaaa cggtcagctt ttccgtttgc gaagcaacca cctcaccctg ctcgttgagc
     6781 aaaataactt ttacgcccga ggtgccaaga tctatcccga tatacatatc gatcgttcct
     6841 taaaaaaatg cccggtatcg ctaccgataa ccgggccaac ggactgcaca gttagccgtt
     6901 atttgtcgaa cagataatga tttaccagat tttccagttg ctcctggcga ccactctgat
     6961 gcaccggaga caaattatgt tcctgagcat atttggctaa atctgccagt gacatttggc
     7021 ctttcaggat ttgctggccc aattcgctat tccagccgga ataacgctgc gcgatgcgtt
     7081 tatccagctc gccatcttca atcatgcgcg ctgcaatttt cagcgccagt gccatcgtat
     7141 ccatcgcgcc gatatgaccg taaaacagat catatttatc agtactttga cgacgtactt
     7201 tggcatcgaa gttcagacca ccggtggtga aaccgcctgc tttgagaatt tcatacatca
     7261 ccagcgcatt ctcttccaca ctgttcggga actggtcggt gtcccagccc agttgcgcat
     7321 cgccacggtt ggcgtcgaca gaaccgaaca ggccaagcgc aatggcggtg gctatttcat
     7381 gatggaaaga gtgacctgcc agcgtcgcgt ggttagcttc aatgttcagt ttaatctctt
     7441 tttccagacc aaactgtttc aggaagccat agaccgtcgc ggcatcgtaa tcatattgat
     7501 gtttagtcgg ttcttgcggt ttcggttcga taagcaacgt gccctagaag ccgattttat
     7561 gtttatgctc aaccaccatc tgcataaagc ggcccagttg ttcacgctcc tgacgcaagt
     7621 cggtatttaa cagcgtttcg taaccttcac gaccacccca caggacatag ttttcaccgc
     7681 ccaatttatg ggttgcttcc atcgctgtaa caacttgcgt tgccgcccag ctgaagactt
     7741 caggatctgg gttcgtcgcc gcacccgcgc catagcgagg gtttgtaaag cagttagcgg
     7801 ttccccacag cagcttcacg ccgctctctt cttgcttggc tgccaggaca tcaaccattt
     7861 gcgcaaaatt attgatgtac tcttttaacg acgcgccctc aggggaaaca tccacatcgt
     7921 ggaagcaata aaatggcaca tgtaacttgt ggaaaaactc aaatgcgaca tctgctttac
     7981 gcttcgccaa cgccagtgcc tcaccaggct gctgccacgg acgattaaac gcccccacac
     8041 caaacatgtc cgccccgttc cagcagaagg tgtgccagta gcaggcggca aaacgcaagt
     8101 gctcttccat acgcttaccc aacaccagtt cgtcgggatt gtagtgacgg aatgctaacg
     8161 ggtttgagga ttttgagcct tcataacgaa cgcgatcgag ctggtcaaaa taggcttgca
     8221 tattgaactc cataatcagg taatgccgcg ggtgatggat gatgtcgtaa tattgggcac
     8281 tccctttcaa ttgctcaatt atgttatttc acactgctat tgagataatt cacaagtgtg
     8341 cgctcgctcg caaaataaaa tgggatgatg aaaatgggta attcctcgaa gataaaaatg
     8401 caataagtac aattgcgcaa caaaagtaag atctcggtca taaatcaaga aataaaccaa
     8461 aaatcgtaat cgaaagataa aaatctgtaa ttgttttccc ctgtttagtt gctaaaaatt
     8521 ggttacgttt atcgcggtga ttgttactta ttaaagctgt cctctaacta cagaaggccc
     8581 tacaccatga aaataaagaa cattctactc accctttgca cctcacttct gcttaccaac
     8641 gttgcggcac acgccaaaga agtcaaaata ggtatggcga ttgatgatct ccgtcttgaa
     8701 cgctggcaaa aagatcgaga tatttttgtg aaaaaggcag aatctctcgg tgcgaaagta
     8761 tttgtacagt ctgcaaatgg caatgaagaa acacaaatgt cgcagattga aaacatgatt
     8821 aaccggggtg tcgatgttct tgtcattatt ccgtataacg gtcaggtatt aagtaacgtt
     8881 gtaaaaaaag ccaaacaaga aggtattaaa gtattagctt acgaccgtat gattaacgat
     8941 gcggatatcg atttttatat ttctttcgat aacgaaaaag tcggtgaact gcaggcaaaa
     9001 gccttggtcg atattgttcc gcaaggtaat tacttcctga tgggcggctc gccggtagat
     9061 aacaacgcca agctgttccg cgccggacaa atgaaagtgt taaaacctta cgttgattct
     9121 ggaaaaatta aagtcgttgg tgaccaatgg gttgatggct ggttaccgga aaacgcattg
     9181 aaaattatgg aaaacgcgct aaccgccaat aataacaaaa ttgatgctgt agttgcctca
     9241 aacgatgcca ccgcaggtgg ggcaattcag gcattaagcg cgcaaggttt atcagggaaa
     9301 gtagcaattt ccggccagga tgcggatctc gcaggtatta aacgtattgc tgccggtacg
     9361 caaactatga cggtgtataa acctattaca ttgctggcaa atactgccgc agaaattgcc
     9421 gttgaattgg gcaatggtca ggagccaaaa gcagatacct cactgaataa tggcctgaaa
     9481 gatgtcccct cccgcctcct gacaccgatc gatgtgaata aaaacaacat caaagatacg
     9541 gtaattaaag acggattcca caaagagagc gagctgtaag cgttacgccc cggcgcggag
     9601 cgggggcgtg atttctctcc atgccgcgtg aatgaattgg cttaggtgga gtcgttatgc
     9661 cttatctact tgaaatgaag aacattacca aaaccttcgg cagtgtgaag gcgattgata
     9721 acgtcagctt gcggttgaat gctggcgaaa tcgtctcact ctgtggggaa aatgggtctg
     9781 gtaaatcaac gctgatgaaa gtgctgtgtg gtatttatcc ccatggctcc tacgaaggcg
     9841 aaattatttt tgcgggagaa gagattcagg cgagtcacat ccgcgatacc gaacgcaaag
     9901 gtatcgccat cattcaccag gaattggccc tggtgaaaga attgaccgtg ctggaaaata
     9961 tcttcctggg taacgaaata acccacaatg gcattatgga ttatgacctg atgacgctac
    10021 gctatcagaa gctgctcgca caggtcagtt tatccatttc acctgatacc cgcgttggcg
    10081 atttagggct tgggcaacaa caactggttg aaattgccaa ggcacttaat aaacaggtgc
    10141 gcttgttaat tctcgatgaa ccgacagcct cattaactga gcaggaaacg tcggttttac
    10201 tggatattat tcgcgatcta caacagcacg gtatcgcctg tatttatatt tcgcacaaac
    10261 tcaacgaagt caaagcgatt tccgatacga tttgcgttat tcgcgacgga cagcacattg
    10321 gtacgcgtga tgctgccgga atgagtgaag acgatattat caccatgatg gtcgagcgag
    10381 agttaaccgc gatttaccct aatgaaccac ataccaccgg agatgaaata ttacgtattg
    10441 aacatctgac ggcatggcat ccggtcaatc gtcatattaa acgagttaat gatgtctcgt
    10501 tttccctgaa acgtggcgaa atactgggta ttgccggact cgttggtgcc ggacgtaccg
    10561 agaccattca gtgcctgttt ggcgtgtggc ccggacaatg ggaaggaaaa atttatattg
    10621 atggcaaaca ggtagatatt cgtaactgtc agcaagccat cacccagggg attgcgatgg
    10681 tacccgaaga cagaaagcgc gacggcatcg ttccggtaat ggcggttggt aaaaatatta
    10741 ccctcgccgc actcaataaa tttaccggtg gcattagcca gcttgatgac gcggcagagc
    10801 aaaaatgtat tctggaatca attcagcaac tcaaagttaa aacgtcgtcc cccgaccttg
    10861 ctattggacg tttgagcggc ggcaatcagc aaaaagcgat cctcgcccgc tgtctgttac
    10921 ttaacccgcg catcctcatt cttgatgaac ctaccagggg tattgatatt ggcgcgaaat
    10981 atgagatcta caaattaatt aaccaactcg tccagcaggg tattgccgtt attgtcatct
    11041 cttccgaatt acctgaagtg ctcggcctta gcgatcgtgt actggtgatg catgaaggga
    11101 aactaaaagc caacctgata aatcataacc tgactcagga gcaggtgatg gaagccgcat
    11161 tgaggagcga acatcatgtc gaaaagcaat ccgtctgaag tgaaattggc cgtaccgaca
    11221 tccggtggct tttctgggct gaaatcactg aatttgcagg tcttcgtgat gattgcagct
    11281 atcatcgcaa tcatgctgtt ctttacctgg accaccgatg gtgcctactt aagcgcccgt
    11341 aacgtctcca acctgttacg ccagaccgcg attaccggca tcctcgcggt aggaatggtg
    11401 ttcgtcataa tttctgctga aatcgacctt tccgtcggct caatgatggg gctattaggt
    11461 ggcgtcgcgg cgatttgtga cgtctggtta ggctggcctt tgccacttac catcattgtg
    11521 acgttggttc tgggactgct tctcggtgcc tggaacggat ggtgggtcgc gtaccgcaaa
    11581 gtcccttcat ttattgtcac cctcgcgggc atgttggcat ttcgcggcat actcattggc
    11641 atcaccaacg gcacgactgt atcccccacc agcgccgcga tgtcacaaat tgggcaaagc
    11701 tatctccccg ccagtaccgg cttcatcatt ggcgcgcttg gcttaatggc ttttgttggt
    11761 tggcaatggc gcggaagaat gcgccgtcag gctttgggtt tacagtctcc ggcctctacc
    11821 gcagtagtcg gtcgccaagc tttaaccgct atcatcgtat taggcgcaat ctggctgttg
    11881 aatgattacc gtggcgttcc cactcctgtt ctgctgctga cgttgctgtt actcggcgga
    11941 atgtttatgg caacgcggac ggcatttgga cgacgcattt atgccatcgg cggcaatctg
    12001 gaagcagcac gtctctccgg gattaacgtt gaacgcacca aacttgccgt gttcgcgatt
    12061 aacggattaa tggtagccat cgccggatta atccttagtt ctcgacttgg cgctggttca
    12121 ccttctgcgg gaaatatcgc cgaactggac gcaattgcag catgcgtgat tggcggcacc
    12181 agcctggctg gcggtgtggg aagcgttgcc ggagcagtaa tgggggcatt tatcatggct
    12241 tcactggata acggcatgag tatgatggat gtaccgacct tctggcagta tatcgttaaa
    12301 ggtgcgattc tgttgctggc agtatggatg gactccgcaa ccaaacgccg ttcttgattt
    12361 tgataaaaat tttctcaaag ccggttacgt attaccggtt ttgagttttt gcatgattca
    12421 gcaggaaaag aaccatgttt actaaacgtc accgcatcac attactgttc aatgccaata
    12481 aagcctatga ccggcaggta gtagaaggcg taggggaata tttacaggca tcacaatcag
    12541 aatgggatat tttcattgaa gaagatttcc gcgcccgcat tgataaaatc aaggactggt
    12601 taggagatgt cgtcattgcc gacttcgacg acaaacagat cgagcaagcg ctggctgatg
    12661 tcgacgtccc cattgttggg gttggcggct cgtatcacct tgcagaaagt tacccacccg
    12721 ttcattacat tgccaccgat aactatgcgc tggttgaaag cgcatttttg catttaaaag
    12781 agaaaggcgt taaccgcttt gctttttatg gtcttccgga atcaagcggc aaacgttggg
    12841 ccactgaacg cgaatatgca tttcgtcagc ttgtcgccga agaaaagtat cgcggagtgg
    12901 tttatcaggg gttagaaacc gcgccagaga actggcaaca cgcgcaaaat cggctggcag
    12961 actgactaca aacgctgcca ccgcaaaccg ggattattgc cgttactgac gcccgagcgc
    13021 ggcatattct gcaagtatgt gaacatctac atattcccgt accggaaaaa ttatgcgtga
    13081 ttggcatcga taacgaagaa ctgacccgct atctgtcgcg tgtcgccctt tcttcggtcg
    13141 ctcagggcgc gcggcaaatg ggctatcagg cggcaaaact gttgcatcga ttattagata
    13201 aagaagaaat gccgctacag cgaattttgg tcccaccagt tcgcgtcatt gaacggcgct
    13261 caacagatta tcgctcgctg accgatcccg ccgttattca ggccatgcat tacattcgta
    13321 atcacgcctg taaagggatt aaagtggatc aggtactgga tgcggtcggg atctcgcgct
    13381 ccaatcttga gaagcgtttt aaagaagagg tgggtgaaac catccatgca atgattcatg
    13441 ccgagaagct ggagaaagcg cgcagtctgc tgatttcaac caccttgtcg atcaatgaga
    13501 tatcgcaaat gtgcggttat ccatcgctgc aatatttcta ctctgttttt aaaaaagcat
    13561 atgacacaac gccaaaagag tatcgcgatg taaatagcga ggtcatgttg tagcgatgat
    13621 gagaattgtc ggcgtcacat cagggaactc agcacagcca cctgatgcga gcaaaagcct
    13681 tatccgaccg acaaatcaga taaggctcga ggaaatgaaa ttacatatgc gccgcgatta
    13741 accgttggtt atcctggtac attgcgaaca ggtagttgtt ataactcttc cctttggtcg
    13801 agtagccctt cagcttgtga atcatcgctg tggcagtcac ttcctgatcc gctttacgca
    13861 gctgtgcacg cgatttacgg aacgaagagt aagccgggtg cgtattcagg ttagtgacat
    13921 aggcgctcac cgattctttg acagaactaa actgtgagta ccctttcact ttacccggcg
    13981 cattggtaca acgtcctttc atgcatttca tgccgaacag attgttattg ttgcgcgcca
    14041 gcttcgacgt tccccaaccg ctttctgctg cagccatcgt cgccaccata ctggtaggga
    14101 taatgtctac gcgttcaagc aaggtattcc acgggatttt tcgcgtatta ccagaccact
    14161 tcaccttgta gcgtttggcg atgtctttca gacgcgcacg ctcagcaggt gcccattggc
    14221 cctgatactg ttttgaaatc agccagttac gttctgcagt aatggctgca ttttggctgg
    14281 taatgtaagg cattacggtc cggagaaacg cctttttcct tggtgttccg gaagggtatt
    14341 ttcgcaaatc aggaagtgaa ctactctttg cactattgcg agaatactct tgtttactgc
    14401 ttacctgttt attactagct ttagttaagt gggacttttg actcgctgtt gttgtgtgcg
    14461 tcttcgctaa cacctcactc gaaaacacca gagtgagtaa cataagaatc atcgccccat
    14521 atcgtcgtat gggagtcaaa atcatcaggt ctcctggtcg gatttaatca ttccaacacc
    14581 ttatattttt cacaaatttg agagttgaat ctcaaatcat atcaaaaata gctgtcaaga
    14641 gcaccccaag gaatagtcca aatctgaaac tatgtcacga gttaacgatt cagattggcg
    14701 ctaaatcgca gaaaatgtgg gggttatcgc aaaattcagc cgttttttgc gcgcgatcgc
    14761 tcacccttgc ttctcatcct gtggacttac cgctcaggga tgagttttgt ctggcttatc
    14821 gctggcaaac tgtctgaaat cgcagcaata aggactcatc caccatgaaa ctcgccgcct
    14881 gttttctgac actccttcct ggcttcgccg ttgccgccag ctggacttct ccggggttcc
    14941 ctgcctttag cgaacaggga acgggaacat ttgtcagcca cgcgcagttg cccaaaggta
    15001 cgcgtccact cacgctaaat tttgaccaac agtgctggca gcctgcagat gcgataaaac
    15061 tcaatcagat gctttccctg caaccttgta gcaacacgcc gcctcaatgg cgattgttca
    15121 gggacggcaa atatacgctg caaatagaca cccgctccgg tacgccaaca ttgatgattt
    15181 ccatccagaa cgccgccgaa ccggtagcaa acctggtccg tgaatgcccg aaatgggatg
    15241 gattaccgct cacgctggat gtcagcgcca ctttcccgga aggagccgcc gtacgggatt
    15301 attacagcca acaaattgcg atagtgaaga acggtcaaat aacgttacaa cccgctgcta
    15361 ccagcaacgg tttactcctg ctggaacggg cagaaactga cacatccgcc cctttcgact
    15421 ggcataacgc cacggtttac tttgtgctga cagatcgttt cgaaaacggc gatcccagta
    15481 atgaccagag ttacggacgt cataaagacg gtatggcgga aattggcact tttcacggcg
    15541 gcgatttacg cggcctgacc aacaaactgg attacctcca gcagttgggc gttaatgctt
    15601 tatggataag cgccccattt gagcaaattc acggctgggt cggcggtggt acaaaaggcg
    15661 atttcccgca ttatgcctac cacggttatt acacacagga ctggacgaat cttgatgcca
    15721 atatgggcaa cgaagccgat ctacggacgc tggttgatag cgcacatcag cgcggtattc
    15781 gtattctctt tgatgtcgtg atgaaccaca ccagctatgc cacgctggcg gatatgcagg
    15841 agtatcagtt tggcgcgtta tatctttctg gtgacgaagt gaaaaaaacg ctgggtgaac
    15901 gctggagcga ctggaaacct gccgccggac aaacctggca tagctttaac gattacatta
    15961 atttcagcga caaaacaggc tgggataaat ggtggggaaa aaactggatc agaacggata
    16021 tcggcgatta cgacaatcct ggattcgatg atctcactat gtcgctggcc tttttgccgg
    16081 atatcaaaac cgaatcaact accgcttctg gtctgccggt gttctataaa aacaaaacgg
    16141 atactcacgc taaagtcatc gaaggcttta cacctcgcga ttacttaacc cactggttaa
    16201 gtcagtgggt ccgcgactat gggattgatg gttttcgggt cgataccgcc aaacatgttg
    16261 agttgcccgc ctggcagcaa ctgaaaaccg aagccagctc cgcgcttcgc gaatggaaaa
    16321 aaactaaccc cgacaaagca ttagatgaca aacctttctg gatgaccggt gaagcctggg
    16381 gccacggcgt gatgcaaagt gactactatc gccacggctt cgatgcgatg atcaatttcg
    16441 attatcagga gcaggcggcg aaagcagtcg attgtctggc gcagatggat acgacctggc
    16501 agcaaatggc ggagaaattg cagggtttca acgtgttgag ctacctctcg tcgcatgata
    16561 cccgtctgtt ccgtgaaggg ggcgacaaag cagcagagtt attactatta gcgccaggcg
    16621 cggtacaaat cttttatggc gatgaatcct cgcgtccgtt cggtcctaca ggttctgatc
    16681 cgctgcaagg tacacgttcg gatatgaact ggcaggatgt tagcggtaaa tctgccgcca
    16741 acgtcgcgca ctggcagaaa atcagccagt tccgcgcccg ccatcccgca attggcgcgg
    16801 gcaaacaaac gacactttcg ctgaagcagg gctacggctt tgttcgtgag catggcgacg
    16861 ataaagtgct ggtcatctgg gctgggcaac agtgactttt ccggcttccc gttcgtcagt
    16921 acctcgggaa gccgccaacc aggataaaat gtcagcccta atcagcgttg cgggataaag
    16981 caccgctcac tcttcaacag accgatttgc accccagcaa atgtagcgtt attgttacct
    17041 tccttgctac agagttcgac agatatcccg ctatgacatt ctcccttttt ggtgacaaat
    17101 ttacccgcca ctccggcatt acgctgctga tggaagatct gaacgacggt ttacgcacgc
    17161 ctggcgcgat tatgctcggc ggcggtaatc cggcgcagat cccggaaatg caggactact
    17221 tccagacgct actgaccgac atgctggaaa gtggcaaagc gactgatgca ctgtgtaact
    17281 acgacggtcc acaggggaaa acggagctac tcacactgct tgccggaatg ctgcgcgaga
    17341 agttgggttg ggatatcgaa ccacagaata ttgcactaac aaacggcagc cagagcgcgt
    17401 ttttctactt atttaacctg tttgccggac gccgtgccga tggtcgggtc aaaaaagtgc
    17461 tgttcccgct ggcaccggaa tacattggct acgctgacgc cggactggaa gaagatctgt
    17521 ttgtctctgc gcgtccgaat attgaactgc tgccggaagg ccagtttaaa taccacgtcg
    17581 attttgagca tctgcatatt ggcgaagaaa ccgggatgat ttgcgtctcc cggccgacga
    17641 atccaacagg taatgtgatt actgacgaag agttgctgaa gcttgacgcg ctggcgaatc
    17701 aacacggcat tccgctggtg attgataacg cttatggcgt cccgttcccg ggtatcatct
    17761 tcagtgaagg gcgcccgcta tggaatccga atatcgtact gtgcatgagt ctttccaagc
    17821 tggggctgcc tggctcccgc tgcggcatta tcatcgccaa tgaaaaaatc atcactgcca
    17881 tcaccaatat gaacggcatt atcagcctgg cacctggcgg tattggtccg gcgatgatgt
    17941 gtgaaatgat taagcgtaac gatctgctgc gcctgtctga aacagtcatc aaaccgtttt
    18001 actaccagcg tgttcaggaa actatcgcca tcattcgccg ctatttaccg gaaaatcgct
    18061 gcctgattca taaaccggaa ggagccattt tcctctggct atggtttaag gatttgccca
    18121 ttacgaccga gcagctctat cagcgcctga aagcacgcgg cgtgctgatg gtgccggggc
    18181 acaacttctt cccgggactg gataaaccgt ggccgcatac gcatcaatgt atgcgcatga
    18241 actacgtacc agagccggag aaaattgagg cgggggtgaa gattctggcg gaagagatag
    18301 aaagagcctg ggctgaaagt cactaaaaat tgcctgatgc gctacgctta tcaggcctac
    18361 atgatttctg caatatattg aatttggaag aatttgtagg ccggataagg cgtttacgcc
    18421 gcatctggca taaacaaagc gcactattcc ggcattgaga aacgccggaa aacgtttcaa
    18481 acaggctgct gctgtagccg atgccgctgc actttctcga catccatgca ctgcaacgcc
    18541 tttgtcgggc aggcttcaac acaggccggc cccgtctccc gatgccagca cagatcgcac
    18601 ttaatcgccc tcgccttttt gcgcgaagaa acgacctcca tcgcaccaaa cgggcaagcc
    18661 agcatacagc ttttacagcc aatgcaacgt gtttgttcaa cgaaaatatg cccatgttcg
    18721 cggcttatcg cgtcaacagg gcagacattc gcgcacggtg catcttcaca ctgatgacag
    18781 gctactggcg tggtccagct gtggtcttta atgacacgaa tacgggaaat aaactcgtct
    18841 ggtgacaacg cagcgcaatc ctgattctcc tgatgcgaca ctgcgcaagc cacttcacag
    18901 gtacggcaac cgatacattt cgtcgcatcc gcaataataa accggttcat gatcattcct
    18961 ttgcactaaa acagcagagt gccagagagc gcgtccctac tgctttgatc caacgaagga
    19021 aatgttaaag ttttggcact tgtccaaagc gttatgtgat cgcgcccagg tcatccctga
    19081 cagtaaatcc cagttcatta gaaatagcct gcgcggtttc acgcagtggt ttcaggagat
    19141 ttttctctcc cacctgtttc agacgtgatg tcgaaagcga aatcgacacg gcgtacggca
    19201 cccgcccatg aatatcaaac accggaacag caatacagga gacgccgagt tcgttttctt
    19261 ctctgtccat cgccgctccg ctttcacgaa tgtgcgccag ttcgtcgaac atcgcgggca
    19321 gctcggtaat ggtattgcgg gttaacggct ggatctcatg ctgatggttt tcccagtatg
    19381 acttcacgta gtccgggtga ccaaacgcca tgtagatctt gcccattgcg gaacagtaga
    19441 gcggcatatg ctggccaata taggcacggg ttcgcagcat cccggttgtg ggttccagct
    19501 tataaatcaa aatagcgtga tcgtcttcgc ggctggagaa gttaatggtt tcaccagtgg
    19561 cgatgttcag tgcctcaaga tgcggagcgg cgatatgaat gatattcagc gaagacagcg
    19621 ccttctgccc gacggcaata aatttggtag tcaggcggta actccctgcg gcgggcgcgg
    19681 tggtcacata gccgcaggac tgtaatccct gcaataagcg atggacggta ctcttattta
    19741 aaccagccag ctccgaaaga tgcgccaacg gacaaccgtt tggatagttg ctcaaaatct
    19801 caatcagcat caacccgcga aacagactct ggcttccggc tggacgctct ttttcctgcg
    19861 ccatctcgtt ctcttttttt cccatcactt ctttccccat tttgtcgcgt cctgatggta
    19921 gcgcaaagtg tgccgtagtt cacgatctcg acagataatt tataaccact tgatttttat
    19981 gtcttttgaa attcatcaat cagattgctt tgttaaaaag tgatcgatat atttgaaatc
    20041 tagttccgca tattgaaatt ttaagccaaa aaagcgatca aaaaacaagg aagcctagga
    20101 tgaaagtgac atttgagcag ttaaaagcag cctttaatcg ggtcttaatt tcacgcggcg
    20161 ttgacagcga aacggctgac gcctgtgcag agatgttcgc ccgcaccacc gaatccggcg
    20221 tttattctca cggcgttaat cgtttccctc gtttcattca acaactggaa aacggcgata
    20281 tcattcctga tgcccaaccc aaacgtataa ccagcctcgg cgcaattgaa cagtgggatg
    20341 cccagcgttc gatcggcaac ctgacggcga aaaagatgat ggatcgcgcc attgaactgg
    20401 ctgccgatca cggtattggt ctggtggcac ttcgtaatgc caaccactgg atgcgcggcg
    20461 gcagctacgg ctggcaggcg gcggaaaaag gctatattgg catttgctgg accaactcca
    20521 tcgccgtaat gccgccgtgg ggcgcaaaag agtgtcgcat cggcactaac ccgctgatcg
    20581 tcgccattcc ttccacgccg atcaccatgg tcgatatgtc gatgtcgatg ttctcttacg
    20641 gcatgttaga agttaaccgc ctggcaggcc gtcagctccc ggtcgatggt ggctttgatg
    20701 atgagggcaa tttgaccaaa gaacctggcg ttatcgagaa gaatcgccgc attttgccga
    20761 tgggctactg gaaaggttct ggcatgtcga ttgtgctgga tatgatcgct actctccttt
    20821 ccgacggcgc atcggttgcc gaagtcaccc aggacaacag cgacgaatac ggcatttcac
    20881 aaatttttat tgccattgaa gtggacaagc ttatcgacgg tcccacccgc gatgccaagc
    20941 tgcaacgtat catggattac gttactaccg ctgaacgcgc tgacgaaaac caggccatcc
    21001 gcttacccgg ccacgaattt actaccctgc tggccgaaaa ccgccgtaac ggcatcactg
    21061 ttgatgacag cgtgtgggcc aaaatccagg cgttatgagg agataagtca tgatttttgg
    21121 acatatcgcg cagcccaatc cgtgccgttt gcccgccgcc attgaaaagg cgctcgattt
    21181 tctgcgcgct accaacttca acgtcctgga gccgggcgtt gtcgaaatcg acggcaagaa
    21241 tatttatgcg c
//
LOCUS       DAJTSI010000066        20972 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000066 DAJTSI010000000
VERSION     DAJTSI010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20972
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.066"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..122
                     /locus_tag="OO327_002174"
     CDS             <1..122
                     /locus_tag="OO327_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCR6018711.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
     gene            138..686
                     /gene="wcaF"
                     /locus_tag="OO327_002175"
     CDS             138..686
                     /gene="wcaF"
                     /locus_tag="OO327_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR6018712.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            712..1833
                     /gene="gmd"
                     /locus_tag="OO327_002176"
     CDS             712..1833
                     /gene="gmd"
                     /locus_tag="OO327_002176"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR6018713.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            1836..2804
                     /gene="fcl"
                     /locus_tag="OO327_002177"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             1836..2804
                     /gene="fcl"
                     /locus_tag="OO327_002177"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR6018714.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRT
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            2804..3283
                     /gene="gmm"
                     /locus_tag="OO327_002178"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             2804..3283
                     /gene="gmm"
                     /locus_tag="OO327_002178"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR6018715.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            3280..4503
                     /gene="wcaI"
                     /locus_tag="OO327_002179"
     CDS             3280..4503
                     /gene="wcaI"
                     /locus_tag="OO327_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCR6018716.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            4506..5942
                     /gene="cpsB"
                     /locus_tag="OO327_002180"
     CDS             4506..5942
                     /gene="cpsB"
                     /locus_tag="OO327_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR6018717.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            6135..7505
                     /gene="cpsG"
                     /locus_tag="OO327_002181"
                     /gene_synonym="manB"
     CDS             6135..7505
                     /gene="cpsG"
                     /locus_tag="OO327_002181"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR6018718.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            7560..8954
                     /gene="wcaJ"
                     /locus_tag="OO327_002182"
     CDS             7560..8954
                     /gene="wcaJ"
                     /locus_tag="OO327_002182"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR6018719.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            8956..10434
                     /gene="wzxC"
                     /locus_tag="OO327_002183"
     CDS             8956..10434
                     /gene="wzxC"
                     /locus_tag="OO327_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCR6018720.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            10614..11894
                     /gene="wcaK"
                     /locus_tag="OO327_002184"
     CDS             10614..11894
                     /gene="wcaK"
                     /locus_tag="OO327_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCR6018721.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            11891..13111
                     /gene="wcaL"
                     /locus_tag="OO327_002185"
     CDS             11891..13111
                     /gene="wcaL"
                     /locus_tag="OO327_002185"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCR6018722.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            13122..14516
                     /gene="wcaM"
                     /locus_tag="OO327_002186"
     CDS             13122..14516
                     /gene="wcaM"
                     /locus_tag="OO327_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCR6018723.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            14691..15584
                     /gene="galF"
                     /locus_tag="OO327_002187"
     CDS             14691..15584
                     /gene="galF"
                     /locus_tag="OO327_002187"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCR6018724.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            15957..17042
                     /gene="rfbB"
                     /locus_tag="OO327_002188"
     CDS             15957..17042
                     /gene="rfbB"
                     /locus_tag="OO327_002188"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR6018725.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            17042..17941
                     /gene="rfbD"
                     /locus_tag="OO327_002189"
     CDS             17042..17941
                     /gene="rfbD"
                     /locus_tag="OO327_002189"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR6018726.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            18000..18878
                     /gene="rfbA"
                     /locus_tag="OO327_002190"
     CDS             18000..18878
                     /gene="rfbA"
                     /locus_tag="OO327_002190"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR6018727.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            18883..19428
                     /gene="rfbC"
                     /locus_tag="OO327_002191"
     CDS             18883..19428
                     /gene="rfbC"
                     /locus_tag="OO327_002191"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR6018728.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            19444..20700
                     /gene="wzx"
                     /locus_tag="OO327_002192"
     CDS             19444..20700
                     /gene="wzx"
                     /locus_tag="OO327_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HCR6018729.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            20730..>20972
                     /locus_tag="OO327_002193"
     CDS             20730..>20972
                     /locus_tag="OO327_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR6018730.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGA"
BASE COUNT         5303 a         4912 c         5591 g         5166 t
ORIGIN      
        1 gtgctgacgc caaaaaagtc caacggcaga tattacatgt acctggcttc tgggcagaat
       61 tatcctggca tttacgccaa cgtacgacag gtaagactaa agccttatat aacaaagtct
      121 gaatataagg aaaaccaatg caagatttaa gcggtttctc ggtgccgaaa gggttccggg
      181 gcggcaacgc tattaaagtg caattatggt gggcagtaca ggcaacaata tttgcctggt
      241 cgccacaagt attgtatcgc tggcgggctt ttttattacg tttattcgga gcaaaaatag
      301 gaaaaaacgt agttattcgt ccgtcagtaa aaattaccta tccgtggaaa ttaaccttag
      361 gtgattacgc gtgggttggc gatgacgtca atttatatac cctcggtgaa ataaccattg
      421 gcgcacattc ggtgatatcg caaaaaagtt atttatgcac cggtagccac gaccatgcaa
      481 gtcaacattt caccattaac gccacgccta ttgtgattgg cgagaaatgc tggctggcaa
      541 ccgatgtctt tgttgcccca ggcgtcacaa tcggcgacgg caccgtcgtg ggtgcacgaa
      601 gcagtgtttt taaatcgctt ccggcaaatg tggtttgccg ggggaatccc gcagtggtga
      661 tacgcgaacg cgttgaaact gaataaattc aaaaatacag aggaataaga catgtcaaaa
      721 gtcgctctca tcaccggtgt aaccggacaa gacggttctt acctggcaga gtttctgctg
      781 gaaaaaggtt acgaggtgca tggtattaag cgtcgcgcat cgtcattcaa caccgagcgc
      841 gtggatcaca tttatcagga tccgcacacc tgcaacccga aattccatct gcattatggc
      901 gacctgagtg atacctctaa cctgacgcgc attttgcgtg aagtacagcc ggatgaagtg
      961 tacaacctag gcgcaatgag ccacgttgcg gtctcttttg agtcaccaga atataccgct
     1021 gacgtcgacg cgatgggtac gctgcgcctg ctggaggcga tccgcttcct cggtctggaa
     1081 aagaaaactc gtttctatca ggcttccacc tctgaactgt atggtctggt gcaggaaatt
     1141 ccgcagaaag aaaccacgcc gttctacccg cgatctccgt atgcggtcgc caaactgtac
     1201 gcctactgga tcaccgttaa ctaccgcgaa tcctacggca tgtacgcttg taacggaatt
     1261 ctcttcaacc atgaatcccc gcgccgcggc gaaaccttcg ttacccgcaa aatcacccgc
     1321 gcaatcgcca acatcgccca ggggctggag tcgtgcctgt acctcggcaa tatggattcc
     1381 ctgcgtgact ggggccacgc caaagactac gtaaaaatgc agtggatgat gctgcaacag
     1441 gaacagccgg aagatttcgt tatcgcgacc ggcgttcagt actccgtacg tcagtttgtg
     1501 gaaatggcgg cagcacagct gggcatcaaa ctgcgctttg aaggcacggg tgttgaagag
     1561 aagggcattg tggtttccgt caccgggcat gacgcgccgg gcgttaaacc gggtgatgtg
     1621 attatcgccg ttgatccgcg ttacttccgt ccggcagaag ttgaaacgct gctcggcgac
     1681 ccgaccaaag cgcacgaaaa actgggctgg aaaccggaaa tcaccctcag agagatggtg
     1741 tctgaaatgg tggctaatga cctcgaagcg gcgaaaaaac actctctgct gaaatctcac
     1801 ggctacgacg tggcgatcgc gctggagtca taagcatgag taaacaacgc atttttatcg
     1861 ctggccattg tgggatggtc ggttctgcca tcaagcggca gctcgaacag cgcggtgatg
     1921 tggagctggt attacgtacc cgcgatgaac tgaacctgct ggacagccgc gccgtgcatg
     1981 atttctttgc cagcgaacgc attgaccagg tctatctggc ggcggcgaaa gtgggcggca
     2041 ttgttgccaa caacacctat ccggcggatt tcatctacca gaacatgatg attgagagca
     2101 acatcattca cgccgcgcat cagaacgacg tgaacaaact gatgtttctc ggatcgtcct
     2161 gcatctaccc gaaactggca aaacagccga tggcagaaag cgagttattg cagggcacgc
     2221 tggagccgac caacgagcct tacgccattg ccaaaatcgc cgggatcaaa ctgtgcgaat
     2281 cttacaaccg ccagtatgga cgcgattacc gcactgtcat gccgaccaac ctgtacgggc
     2341 cgcatgacaa cttccacccg agtaactccc atgtgatacc agcattgctg cgtcgcttcc
     2401 acgaggcgac ggcacagaat gcgccggacg tggtggtatg gggcagcggt acaccgatgc
     2461 gtgaattcct gcacgtcgat gatatggcgg cggcgagcat tcatgtcatg gagctggcgc
     2521 atgaagtctg gctggagaat acccaaccga tgctgtcgca cattaacgtc ggcacgggcg
     2581 ttgactgcac tatccgcgag ctggcgcaaa ccatcgccaa agtggtgggt tacaaaggtc
     2641 gggtggtttt tgatgccagc aaaccggatg gtacgccgcg caaactgctg gatgtgacgc
     2701 gcctgcatca gcttggctgg tatcacgaaa tctcactgga agcggggctt gccagcactt
     2761 accagtggtt ccttgagaat caagaccgct ttcgggggga atgatgtttt tacgtcagga
     2821 agactttgcc actgtagtgc gctccacacc gcttgtctct ctcgacttta ttgtcgagaa
     2881 cagtcgcggc gagtttctgc ttggcaaaag aaccaaccgc ccggcgcagg gttactggtt
     2941 tgtgccggga gggcgcgtgc agaaagacga aacgctggaa gccgcatttg agcggctgac
     3001 gatggcggaa ctggggctgc gtctgccgat aacagcaggc cagttttacg gtgtctggca
     3061 gcatttttat gacgataact tctccggcac ggatttcacc actcactatg tggtgctcgg
     3121 ttttcgcttc agagtagcgg aagaagagct gttactgccg gatgagcagc atgacgatta
     3181 ccgctggctg acgccggacg cgctgctcgc cagtaacgat gttcacgcta acagccgagc
     3241 ctattttctc gctgagaagc gtgccggagt acccggatta tgaaaatact ggtctacggc
     3301 attaactact cgccggagtt aaccggcatc ggcaaataca ccggcgagat ggtggaatgg
     3361 ttggcggcac aaggtcatga ggtgcgggtc attaccgcac cgccttacta cccgcaatgg
     3421 caggtgggtg agaactattc cgcctggcgc tacaaacgag aagaaggggc cgccacggtg
     3481 tggcgctgcc cgctgtacgt gccaaaacag ccgagcaccc tgaaacgctt gttgcatctc
     3541 ggcagttttg ccgtcagcag tttctttcca ctgatggcgc aacgtcgctg gaagccggat
     3601 cgcattattg gcgtagtgcc aacgctgttt tgcacgccgg gaatgcgcct gctggcgaag
     3661 ctctctggtg cgcgtaccgt gctgcatatt caggattacg aagtggacgc catgctgggg
     3721 ctgggccttg ccggaaaagg caaaggcggc aaagtggcac agctggcaac ggcgttcgaa
     3781 cgtagcggac tgcataacgt cgataacgtt tccacgattt cgcgttcgat gatgaataaa
     3841 gccatcgaaa aaggcgtggc ggcggataac gtcatcttct tccccaactg gtcggaaatt
     3901 gcccgttttc agcatgttgc agacgccgat gttgatgccc ttcgtaacca gcttggcctg
     3961 ccggataaca aaaaaatcat tctttactcc ggcaatattg gtgaaaagca ggggctggaa
     4021 aacgttattg aagctgccga tctcctgcgc gatgaaccgc tgatttttgc cattgtcggg
     4081 cagggcggcg gcaaagcgcg gctggaaaaa atggcgcagc agcgtggact gcgcaacatg
     4141 caatttttcc cgctgcaatc gtatgacgct ttacctgcac tgctgaagat gggcgattgc
     4201 catctggtgg tgcaaaaacg cggcgcggca gatgccgtat tgccgtcgaa actgaccaat
     4261 attctggcgg taggcggtaa cgcggtgatt accgcagagg cccacacaga actggggcag
     4321 ctttgcgaaa cctttccggg tattgcggtt tgcgtagaac cggaatcggt cgaggcgctg
     4381 gtggcgggga ttcgtcaggc gctcctgctg cccaaacata acacggtggc acgtgaatat
     4441 gccgaacgca cgctcgataa agagaacgtg ttacgtcaat ttataaatga tattcgggga
     4501 taattatggc gcagtcgaaa ctctatccag ttgtgatggc aggtggctcc ggtagccgtt
     4561 tatggccgct ttcccgcgta ctttacccca agcagttttt atgcctgaaa ggcgatctca
     4621 ccatgctgca aaccaccatc tgccgcctga acggcgtgga gtgcgaaagc ccggtggtga
     4681 tttgcaatga gcagcaccgc tttattgtcg cggaacagct gcgtcaactg aacaaactca
     4741 ccgagaacat tattctcgaa ccggcagggc gtaacactgc acctgccatt gcactggcgg
     4801 cgctggcggc aaaacgtcat agcccggaga gcgacccgtt aatgctggta ttggcggcgg
     4861 atcatgtgat tgccgatgaa gacgcgttcc gtgccgccgt gcgtaatgcc atgccttatg
     4921 ccgaagcggg caagctggtg acctttggca ttgtgccgga tctaccagaa accggttatg
     4981 gctatattcg tcgcggtgaa gtgtctgtgg gtgagcagga tacggtggcc tttgaagtgg
     5041 cgcagtttgt cgaaaaaccg aatctggaaa ccgcccaggc ctatgtagca agcggcgaat
     5101 attactggaa cagcggcatg ttcctgttcc gcgccggacg ctatctcgaa gaactgaaaa
     5161 aatatcgccc ggatatcctc gatgcctgcg aaaaagcgat gagcgccgtc gatccggatc
     5221 tcgattttat tcgtgtggat gaagaagcgt ttctcgcctg cccggaagag tcggtggatt
     5281 acgcggtcat ggaacctacg gcagatgctg ttgtggtgcc gatggatgcg ggctggagcg
     5341 atgttggttc ctggtcttca ttatgggaga tcagcgccca caccgccgag ggcaacgttt
     5401 gccacggcga tgtgattaat cacaaaactg aaaacagcta tgtgtatgcc gaatcaggcc
     5461 tggtcaccac cgtcggggtg aaagatctgg tagtggtgca gaccaaagat gcggtgctga
     5521 ttgccgaccg taacgcggta caggatgtga aaaaagtggt cgagcagatc aaagccgatg
     5581 gtcgccatga gcatcgggtg catcgcgaag tgtatcgtcc gtggggcaaa tatgactcta
     5641 tcgacgcggg cgaccgctac caggtgaaac gcatcaccgt gaaaccgggc gagggtttgt
     5701 cggtacagat gcaccatcac cgcgcggaac actgggtagt ggtcgcggga acggcaaaag
     5761 tcaccattga tggtgatatc aaactgcttg gtgaaaacga gtccatttat attccgctgg
     5821 gggcgacgca ctgcctggaa aacccgggga aaattccgct cgatttaatt gaagtgcgct
     5881 ccggctctta tctcgaagag gatgatgtgg tgcgcttcgc ggatcgctac ggacgggtgt
     5941 aaacgtcgca tcaggcgctg tttgtcggat gcggcgtgaa cgccttatcc gacctacggt
     6001 tcggttttgt aggcctgata agacgcggca gcgtcgcatc aggcaatgaa tgcgaaaccg
     6061 cggtgtaaat aacgacaaaa ataaaattgg ccgtttcggt cagggccaac tattgcctga
     6121 aaaagggtaa cgacatgaaa aaattaacct gctttaaagc ctatgatatt cgcgggaaat
     6181 taggcgaaga actgaatgaa gatatcgcct ggcgcattgg tcgcgcctat ggcgaatttc
     6241 tcaaaccgaa aaccattgtg ttaggcggtg atgtccgtct caccagcgaa accttaaaac
     6301 tggcgctggc aaaaggttta caggatgcgg gcgtcgatgt gctggatatc ggtatgtccg
     6361 gcaccgaaga gatctatttc gccacgttcc atctcggcgt ggatggcggc attgaagtta
     6421 ctgccagcca taatccgatg gattacaacg gcatgaagct ggtgcgcgag ggggctcgca
     6481 cgatcagcgg tgataccgga ctgcgcgacg tccagcgtct ggctgaagcc aacgactttc
     6541 ctcccgtcga tgaaaccaaa cgcggtcgct atcagcaaat caacctgcgt gacgcttacg
     6601 ttgatcacct gttcggttat atcaatgtca aaaatctcac gccgctcaag ctggtgatca
     6661 actccgggaa tggcgcagcg ggtccggtgg tggacgccat tgaagcccgc tttaaagccc
     6721 tcggcgcgcc cgtggaatta atcaaagtac acaacacacc ggacggcaat ttccccaacg
     6781 gtattcctaa cccgctgctg ccggaatgtc gcgacgacac ccgcaatgcg gtcatcaaac
     6841 acggcgcgga tatgggcatt gcctttgatg gcgattttga ccgctgtttt ctgtttgacg
     6901 aaaaagggca gtttatcgag ggctactaca ttgtcggcct gctggcagaa gcgttcctcg
     6961 aaaaaaatcc cggcgcgaag atcatccatg atccgcgtct ctcttggaac actattgatg
     7021 tggtgactgc tgcaggcggc acgccggtaa tgtcgaaaac cggacacgcc tttattaaag
     7081 aacggatgcg caaggaagac gccatctacg gtggcgaaat gagcgcccac cattacttcc
     7141 gtgatttcgc ttactgcgac agcggcatga tcccgtggct gctggtcgcc gaactggtgt
     7201 gcctgaaagg aaaaacgctg ggcgaactgg tgcgcgaccg gatggcggcg tttccggcaa
     7261 gcggtgagat caacagcaaa ctggcgcaac ccgttgaggc gattaaccgc gtggaacagc
     7321 attttagccg tgaggcgctg gcggtggatc gcagcgatgg catcagcatg acctttgccg
     7381 actggcgctt taacctgcgc acctccaata ccgaaccggt ggtgcgcctg aatgtggaat
     7441 cgcgcggtga tgtgccgctg atggaagcgc gaacgcgaac tctgctgacg ttgctgaacg
     7501 agtaatgtcg gatcttccct taccccactg cgggtaaggg gctaataaca ggaacaacga
     7561 tgacaaatct aaaaaagcgc gagcgagcga aaaccaatgc atcgttaatc tctatggtgc
     7621 aacgcttttc agatatcacc atcatgtttg ccgggctatg gctggtttgc gaagtcagcg
     7681 gactgtcatt cctctacatg cacctgttgg tggcgctgat tacgctggtg gtgttccaga
     7741 tgctgggcgg catcaccgat ttttatcgct catggcgcgg tgttcgggca gcgacagaat
     7801 ttgccctgtt gctacaaaac tggaccttaa gcgtgatttt cagcgccgga ctggtggcgt
     7861 tcaacaatga tttcgacacg caactgaaaa tctggctggc gtggtatggg ctgaccagca
     7921 tcggactggt ggtttgccgt tcatgtattc gcattggggc gggctggctg cgtaatcatg
     7981 gctacaacaa gcggatggtc gccgtagcgg gggatttagc cgccggacaa atgctgatgg
     8041 agagtttccg taatcagccg tggttagggt ttgaagtggt gggcgtttac cacgacccga
     8101 aaccgggcgg cgtttctaac gactgggcgg gtaacctgca acagctggtc gaggatgcaa
     8161 aagcgggcaa gattcataac gtctatatcg cgatgcaaat gtgcgacggc gcacgagtga
     8221 aaaaactggt ccatcaactg gcggatacca cctgttcggt gctgctgatc cccgacgtct
     8281 ttaccttcaa cattctccat tcacgtctcg aagagatgaa cggcgttccg gtggtgccgc
     8341 tgtatgacac gccgctttcg ggggttaacc gcctgctaaa acgtgcggaa gacattgtgc
     8401 tggcgacgct tattctgctg ctgatctccc cggtgctgtg ctgtattgcg ctggcggtga
     8461 aactcagttc accagggccg gttattttcc gccagactcg ctatggtatg gatggcaagc
     8521 cgatcaaagt gtggaagttc cgttccatga aagtgatgga gaacgacaaa gtggtgaccc
     8581 aggcgacgca gaacgatccg cgcgtcacta aagtggggaa ctttctgcgc cgcacctcgc
     8641 tggacgaatt gccgcagttt atcaatgtgc tgaccggggg gatgtcgatt gtcggtccac
     8701 gtccgcacgc agtagcgcat aacgaacagt atcgccagct cattgaaggc tacatgctgc
     8761 gccataaagt gaaaccgggc attaccggct gggcgcagat taatggctgg cgcggcgaaa
     8821 ccgacacgct ggagaaaatg gaaaaacgcg tcgagttcga ccttgagtac atccgcgaat
     8881 ggagcgtctg gttcgatatc aaaatcgttt tcctgacggt attcaagggc ttcgttaaca
     8941 aagcggcata ttgatatgag cttacgtgaa aaaaccatca gcggcgcgaa gtggtcggct
     9001 attgccacgg tgatcatcat cggcctcggg ctggtgcaga tgaccgtgct ggcgcggatt
     9061 atcgacaacc accagttcgg cctgctgact gtgtcgctgg tgattatcgc gctggcagat
     9121 acgctttctg acttcggtat cgctaactcg attattcagc gaaaagaaat cagtcacctt
     9181 gaactcacca cgctgtactg gctgaacgtc gggctggggc ttgtggtatg tgtggcggtg
     9241 tttttgttga gtgatgtcat cggcgacgtg ctgaataacc cggacctggc accgttgatt
     9301 aaaacattgt cgctggcgtt tgtggtgatc ccccacgggc aacagttccg cgcgttgatg
     9361 cagaaggagc tggagttcaa caaaatcggc atgatcgaaa ccagcgcggt gctggcgggc
     9421 ttcactttta cggtagttag cgcccatttc tggccgctgg cgatgaccgc gatcctcggt
     9481 tatctggtca atagtgcggt gagaacactg ctgtttggct actttggacg caaaatttat
     9541 tgccccggtc tgcacttctc gctggcgtct gtggcaccga acttacgttt tggtgcctgg
     9601 ctgacggcgg acagcatcat caactatctc aacaccaacc tttcaacgct ggtgctggcg
     9661 cgtattcttg gcgcgggcgt ggcaggggga tacaacctgg cgtacaacgt ggccgtcgta
     9721 ccgccgatga agctgaaccc aatcatcacc cgcgtgttgt ttccggcgtt tgccaaaatt
     9781 caggacgaca ccgaaaagct gcgtgttaac ttctacaagc tgctgtcgat agtggggatt
     9841 atcaactttc cggcgctgct ggggctgatg gtggtgtcga ataacttcgt accgctggta
     9901 ttcggcgaaa agtggaacag cattattccg gtgctgcaat tgctgtgtat tgtgggtctg
     9961 ctgcgctccg tgggtaaccc gattggttcg ctgctgatgg cgaaagcgcg ggtcgatatc
    10021 agctttaaat tcaacgtatt caaaacattt ctgtttattc cggcgattgt tattggtggg
    10081 cagatggcgg gcgcaatcgg cgtcacgctc ggtttcctgc tggtgcagat tatcaacacc
    10141 attctgagtt acttcgtgat gattaaaccg gttcttggtt ccagttatcg ccagtacatc
    10201 ctgagtttgt ggctgccgtt ttatctctcg ttgccgacgc tggtggtcag ttatgcgctg
    10261 ggcattgtgc tgaaagggca actggcgctg gggatgctgc tggcggtgca aatagccgcg
    10321 ggcgtgctgg cgtttgtggt gatgattgtg ctgtcacgcc atccgctggt ggtggaagtg
    10381 aagcgtcagt tttgtcgcag cgaaaaaatg aaaatgcttt tacgggcggg gtgaattgct
    10441 gttttcggtt ccgacaacgg ttgccggatg cggcgtaaaa cgccttatcc gtcctacggt
    10501 tctgtgctcg ggacctgtag gcctgataag acgcgccagc gtcgcatcag gcaaaaaacg
    10561 gcaacgaaca caacaaacac agcctcacct ggcatcaaat ttatgaggaa aaaatgaaat
    10621 tacttattct gggcaaccac acttgcggca atcgtggcga cagcgccatc ttgcgcggct
    10681 tacttgatgc catcaacatc ctcaatccac ataccgaagt ggacgtgatg agccgctatc
    10741 cggtcagttc ttcctggctg ctcaaccgcc cggtaatggg cgatccgctg ttcctgcaga
    10801 tgacacaaca caatagcgcg gcgggcgttg tcgggcgcat taaaaaagtc ctccgtcgcc
    10861 gctaccagca tcaggtattg ctctcacgcg tcaccgacac tggcaagctg cgcaatatcg
    10921 ccatcgccca gggattcacc gacttcgtgc gcctgctgtc aggttacgac gccattattc
    10981 aggtcggcgg atcgtttttt gtcgatctct acggcgtgcc gcagtttgaa catgcgcttt
    11041 gcacatttat ggcgaaaaag ccgctgttta tgattggtca cagcgtcggc cccttccagg
    11101 atgagcaatt taaccaactg gcgaactatg tctttggtca ctgcgacgcg ctgatcctgc
    11161 gcgaatcggt cagccttgat ttgatgaaac gcagaaatat caccaccgca aaagtggaac
    11221 atggcgtcga taccgcgtgg ctggtcgatc accacaccga agacttcacc gccagctatg
    11281 ccgttcaaca ctggctggac gttgccgcac aacagaaaac agtggcaatt accctgcgcg
    11341 aactggcacc gttcgacaaa cgtctcggca ccactcaaca agcgtatgaa aaagcctttg
    11401 ccggggtggt caatcgcatt ctcgatgaag gctatcaggt gattgcgctc tccacctgta
    11461 cgggtatcga cagctataac aaagacgacc gcatggtggc gctcaatctg cgccagcaca
    11521 tcagcgatcc tgcccgttac cacgtagtga tggatgaact caacgatctg gaaatgggca
    11581 aaattctcgg agcctgtgaa ctcaccgtcg gtacgcgcct gcactccgcc attatctcaa
    11641 tgaactttgc tactccggcg attgccatca actatgaaca taaatccgcc gggattatgc
    11701 agcagctggg actgccagag atggcgattg atattcgtca tttactggac ggcagcctgc
    11761 aagcgatggt tgcggatacc ttaggccagc ttccggtgct gaacgctcga tttaacgaag
    11821 ccgtcagtcg cgaacgtcag acgggaatgc agatggtgca gtctgtgctt gagcgcatcg
    11881 gggaggtgaa atgaaggtcg gcttcttctt attgaaattc ccgctgtcgt cggaaacctt
    11941 cgtcctcaat caaattaccg cgtttattga tatgggcttt gaagtggaga ttgtcgcttt
    12001 gcaaaaaggc gatacagaga acacccacgc ggcatggacg aaatacaacc ttgtcgccag
    12061 aacccgctgg ttacaggacg aacctgcggg caaagtagcg aaactgcgcc accgcgccag
    12121 ccagacctta cgtggcattc atcgtaaaaa tacctggcat gcgctcaacc tcaaacgcta
    12181 tggtgccgag tcgcggaacc tgattttgtc tgccatttgt ggtcaggtcg caacaccgtt
    12241 tcgcgccgat gtgttcatcg ctcattttgg cccggcgggg gtaaccgcag caaaactacg
    12301 cgaactgggt atcattcgcg gcaaaattgc caccatcttc cacggtattg atatctccag
    12361 tcgggaagtg ctcaaccact acactcccga atatcaacaa ctgtttcgcc gtggcgacct
    12421 gatgttaccg ataagcgatc tgtgggccgg aaggctgcaa aaaatgggct gccccagaga
    12481 aaaaatcgcc gtatcgcgta tgggcgtgga catgacgcgc tttagcccgc gtccggtgaa
    12541 agcgcccgca acgccgctgg aaatcatatc tgtcgcacgc ttaaccgaga aaaaaggcct
    12601 gcatgtggcg attgaagcct gccgtcagtt gaaagagcag ggcgtggcat ttcgctatcg
    12661 catcctcggc attggcctgt gggaacgacg cctgcgcacc atcatcgaac aatatcaact
    12721 ggaagatgtg gtagagatgc cgggctttaa accgagccat gaagtgaagg agatgctcga
    12781 cgacgcggat gtcttcctgt tgccatcggt aacgggcgcg gatggcgata tggaaggcat
    12841 tccggtggcg ctaatggaag cgatggcggt cggtattccg gtggtttcta ctctgcatag
    12901 cggaattcct gaactggtgg aggccgataa atccggctgg ctgctgcctg agaacgatgc
    12961 tcgcgcactg gcacaacgtc tggcggcgtt tagccaactg gataccgacg aactggctcc
    13021 ggtcgtcaaa cgtgcgtgcg aaaaagtcga acacgatttt aaccagcagg tgattaatcg
    13081 agaactcgcc agcttgctgc aggctttata gaggagaacg tatgccattt aaaacactct
    13141 cccgacgcac cttcctgacg gcaagctcgg cgcttgcctt cctccatacc cctttcgctc
    13201 gcgcacttcc cgcccgacaa agcgttaaca ttaacgacta caacccacat gactggatcg
    13261 cctcatttaa acaagccttc agcgaagggc aaacggtcgt cgtgcctgcc ggattcgttt
    13321 gtgacaatat caacaccggc atcttcatcc ctcccggcaa aacgttacac atccttggaa
    13381 gcctgcgcgg caacggcaga gggcgatttg tcttacagga cggcagccag gtgacagggg
    13441 gagagggcgg cggtatgcat aacattaccc tggatgtgcg tggttctgac tgcaccatca
    13501 aagggctggc gatgagcggc tttggcccgg taatgcagat ttatattggc ggcaaaaaca
    13561 aacgggtcat gcgcaacctg accatcgata acctcactgt cagccacgct aattacgcca
    13621 tcttacgcca gggatttcat aaccagatta tcggtgccaa cattaccaac tgtaagttca
    13681 gcgacttaca gggcgacgcc attgaatgga acgtggcaat taacgacagt gatattttga
    13741 tatctgacca tgtcatcgag cgtatcaact gtaccaacgg caaaatcaac tggggcatcg
    13801 gcataggcct tgcgggaagc acttatgata acaactaccc ggaagaccag gcagtgaaaa
    13861 actttgtcgt ggcgaatatc acgggatcgg attgtcggca gttgatccat gttgaaaatg
    13921 gtaaacattt tgttattcgt aatatcaatg cccgcaatat cacgccggat ttcagtaaga
    13981 aagcgggcat tgataacgcg acagtcgcta tttacggttg tgacaatttc gtgattgata
    14041 atattgaaat gattaatagc gccgggatgt taatcggcta tggggtaatt aaaggcaaat
    14101 atctctcgat accgcaaaat ttccgagtga ataatattca actggataat acccatcttg
    14161 cttataaatt gcgcggcatc caaatctccg ccgggaatgc cgtctccttt gtgtcgctaa
    14221 ctaacattga gatgaagcgt gcctcgctgg agttacacaa caagccgcaa catcttttta
    14281 tgcgaaatat caatgtgatg caggaatcct cagttggacc agcattgagc atgaacttcg
    14341 acatgcgcaa agacgttcgc ggcgtcttta tggcgaaaaa agaaacactg ctgtctcttg
    14401 caaatgttca tgcggtgaat gaaagagggc aaagctccgt cgatatcgac aggattaatc
    14461 accatattgt taatgtggaa aagattaact ttagattgcc ggaacggagg gagtagattt
    14521 gcgaccattc ctggaaaaat ggagccatac ttaggaacaa tgctactgca atccacaacg
    14581 aagcggcgta acatcacaag taattcagta atcaattcag ggtaattgat gctggcgaaa
    14641 aaaatcgaac aagctataat tcagcaacca ttttacaggt ggatgaaata atgacgaatt
    14701 taaaagcagt tattcctgta gcgggtctcg ggatgcatat gttgcctgcc actaaggcga
    14761 tacccaaaga gatgctacca atcgtcgaca agccaatgat tcagtacatt gttgacgaga
    14821 ttgtggctgc agggatcaaa gaaatcctcc tggtaactca cgcgtccaag aacgcggtcg
    14881 aaaaccactt cgacacctct tatgagttag aatcactcct tgagcagcgc gtgaagcgtc
    14941 aactgctggc ggaagtacag tccatctgtc cgccgggcgt gatcattatg aacgtgcgtc
    15001 agggcgaacc tttaggttta ggccactcca ttttgtgtgc gcgacctgcc attggtgaca
    15061 acccatttgt cgtggtactg ccagacgttg tgatcgacga tgccagcgcc gacccgctac
    15121 gttacaacct tgctgccatg attgcacgtt tcaacgaaac gggccgcagc caggtgctgg
    15181 caaaacgtat gccgggtgac ctctctgaat actccgtcat ccagactaaa gagccgctgg
    15241 accgtgaggg taaagtcagc cgcattgttg aatttatcga aaaaccggat cagccgcaga
    15301 cgctggactc agacatcatg gccgtaggtc gctatgtgct ttctgccgat atttggccgg
    15361 aactggaacg tactcagcct ggtgcatggg gacgtattca gctgactgat gctattgccg
    15421 agctggcgaa aaaacaatcc gttgatgcaa tgctgatgac cggcgacagt tacgactgcg
    15481 gcaaaaaaat gggctatatg caggcgtttg tgaagtatgg cctacgcaac ctgaaagaag
    15541 gggcgaagtt ccgtaaaggt attgagaagc tgttaagcga ataatgaaaa tctgaccgga
    15601 tgtaacggtt gataagaaaa ttataacggc agtgaagatt cgtggcataa gttttgctca
    15661 ttgaattttc ctgccgttgc cgtttttata aagcctataa aaacaagaag ttacatagct
    15721 catattattc aaatttttcc aggattttcc ttgtttctac ggctgtttgg taagacaatt
    15781 aacgtttgaa tttttcggat ttggcgcgag tggctaacgc tcgccacatc gttgagatgc
    15841 atgcagtgca ctggtagctg ttaagccagg ggcggtagcg tgtattaata cctctattaa
    15901 tcaaatctgg agcagtctat ttcacagcat gctctatagc tatatggaat aaaaaagtga
    15961 agatacttgt tactggtggc gcagggttta ttggttctgc tgttgttcgt cacataataa
    16021 ataatacgca agatagtgtt gttaatgtcg ataaattaac atacgccggg aacctggaat
    16081 cactcgctga tgtttctgat tctgaacgtt atgcttttga gcatgcagat atctgcgatg
    16141 ctgtagccat gtcgcgtatt ttcgcacagc accagccaga cgcggtgatg cacctggcgg
    16201 cagagagcca cgttgaccgt tcaataacgg gccctgcggc atttattgaa accaatattg
    16261 tggggactta tgttctttta gaagcggcgc gcaattactg gtctgctctg aatgatgaaa
    16321 agaaaaaaag tttccgcttt catcatattt ctactgatga agtttatggt gacttacccc
    16381 atccggatga agcaaataat aacgaagcgt taccgctatt tacggaaacg acagcttacg
    16441 cgccaagtag cccgtattct gcgtctaaag cttccagcga tcatttggta cgtgcctgga
    16501 agcggactta tggtttacca accattgtga ctaattgctc gaacaattat ggtccttatc
    16561 acttcccgga aaaacttatt ccattggtta ttctgaatgc tctggaaggt aaggcattac
    16621 ctatttatgg taaaggggat caaattcgcg actggttgta tgtagaggat catgctcgtg
    16681 cgttatatac cgtcgtaacc gaaggtaaag cgggtgaaac ttataacatt ggtggacata
    16741 acgaaaagaa aaacatcgac gtagtgctca ctatttgtga tttgttggat gagattgtac
    16801 cgaaagagaa atcttatcgt gagcaaatta cttatgttgc cgatcgcccg gggcatgacc
    16861 gccgttacgc cattgatgcc gataaaatta gccgcgaatt gggctggaaa ccgcaagaaa
    16921 cgtttgagag cgggattcgt aaaacggtgg aatggtatct ggctaataca aattgggttg
    16981 agaatgtgaa aagcggtact tatcagtcgt ggattgaaca aaactatgag ggccgtcagt
    17041 aatgaatatc ctgcttttcg gcaaaacagg gcaggtgggt tgggaactgc agcgtgctct
    17101 ggctcctctg ggtaacttga ttgcgctcga tgttcactcc actgattatt gtggagattt
    17161 cagcaacccc gaaggtgtgg ctgaaaccgt caaaaaaatt cgccccgatg ttattgttaa
    17221 tgctgctgct cacactgcag tagacaaagc ggaatcagaa ccgaatttcg cgcaattact
    17281 taacgcgaca tgcgtcgaag cgattgcaaa agcagctaat gaagtcggag cctgggttat
    17341 acactactcc actgattatg ttttcccagg caacggtgac acgccatggc tggaaacgga
    17401 tgcaacagca ccgctaaatg tctacggtga aaccaagcta gctggggaaa aagcgttaca
    17461 agaacattgc gcaaagcatc ttattttccg taccagctgg gtatacgctg gtaaaggaaa
    17521 taactttgcc aaaacgatgt tgcgcctggc aaaagaacgc gaagagttgg ctgtaataaa
    17581 cgatcaattc ggcgcaccaa cgggggctga attactggct gattgcaccg ctcatgccat
    17641 tcgcgtggcc gcaaataaac cagaagttgc tggtttgtac catctggttg ctggtggtac
    17701 aacaacctgg cacgattatg ccgctctggt atttgaagaa gcgcgcagag cggggatcaa
    17761 ccttgctctt aacaaactta atgccgtgcc gacaacggcc tatcccacac cagcacgtcg
    17821 tccccataat tctcgcctca ataccgaaaa atttcagcag aactttgcgc ttgtcttgcc
    17881 tgactggcag gtgggcgtga aacgtatgct caacgaatta tttacgacca cggcaattta
    17941 acaaattatt gcatctcgct catgatgcta gagcgagatg aattaaaagg aatggtgaaa
    18001 tgaaaacgcg taaaggtatt attctggctg gtggttccgg cactcgtctt tatcctgtga
    18061 caatggctgt gagcaaacaa ctgctaccga tttatgataa accgatgatt tattatccgc
    18121 tttcaacgct tatgttggcg ggtattcgag atattctgat tatcagtacg ccgcaggata
    18181 caccacgttt tcaacaattg cttggtgatg gaagccagtg ggggcttaat ctgcaatata
    18241 aagtacaacc gagtccggat ggtctggcgc aagcatttat cattggtgaa gaatttatag
    18301 gtggtgatga ttgtgcgctg gtacttggtg ataatatctt ctatggtcac gacctgccga
    18361 aattaatgga taccgctgtt aacagagaga gtggcgcaac ggtttttgcc tatcacgtaa
    18421 atgatcctga gcgctacggt gtggtggaat ttgatgataa cggtacggca attagtctgg
    18481 aagaaaaacc gcaggaacca aaaagtaact atgcggttac tgggctttat ttctatgaca
    18541 atgacgttgt agaaatggcg aaaaacctta aaccatctgc acgtggcgaa ctggaaatta
    18601 ccgatattaa ccgtatttat atggaccaag gacgtttgtc tgtcgctatg atggggcgtg
    18661 gttatgcatg gctggacacg gggacacatc aaagtcttat tgaagcaagc aacttcattg
    18721 caacaattga agagcgtcag gggctgaaag tttcctgccc ggaagaaatt gctcaccgta
    18781 aaggctttat tgatgctgag caagtgaagg tattagccga gccgctgaag aaaaatgctt
    18841 atggtcagta tctgctgaaa atgattaaag gttattaata aaatgaacgt aattaaaact
    18901 gaaattcctg atgtgctaat ttttgaacca aaagtatttg gtgatgagcg gggctttttt
    18961 atggaaagct tcaatcagaa agttttcgaa gaggctgtag gacgaaaggt tgagtttgtt
    19021 caggataacc attctaaatc aactaagggt gtgttacgcg gactgcacta tcagctggaa
    19081 ccttatgctc aaggaaaact ggtacgctgt gttgtcggtg aagtttttga cgtagctgtt
    19141 gatattcgga aatcatcacc aacatttggc aagtgggttg gtgtaaattt gtcggcggag
    19201 aataagcgtc aattatggat accggaagga ttcgctcatg ggttttgcgt tcttagtgat
    19261 gaagcagaat ttgtctacaa aacaaataat ttctattcaa aaatgcaaga gcgtgggatt
    19321 ttatggagcg acaaaagtat aaatatagaa tggccggttc aaaatccatt gctttctgat
    19381 aaagatatta atggtcaaaa atttgtagat gctgattatt ttatatgata aagaaatgta
    19441 ataatgagca taataaaaaa tagtgtctgg aacctttttg gctatgcaat accaacttta
    19501 attgctatcc cctcgctagg atttctcgct cgaggtttgg ggcctgaagg tttcggtgtt
    19561 tatacaattg caattgcact tgttgggtat gctggaattt ttgatgtagg cctgactcgc
    19621 tctgttatta gagaaattgc aattcatcgt gataatcatc atgaaagaac caaggtaatt
    19681 tcaacaagta catctttttt ggtgctattt tcatgctttg gtgctttttt attattgatt
    19741 ttctctgatg gaattgttaa ttatctaaaa atttctggtg ttgaacatag tgatatacaa
    19801 ctagcattta aactgttggc tatttgcatt ccattattta ttctaaatca attatggtca
    19861 gccattcttg agggggatga aaaatttggc attgtaaata ttcaaaaatc tatatcaagc
    19921 tcttgcatcg cgggaattcc ggccatattt gttttttata gtgctacatt gtcggcagcg
    19981 gttgctggtt taatatttgc aagagttatt tcgattttag tctctgccta ttatgtcagg
    20041 aatgatatta aaatttcggg ggttcatttt tgttataaaa cctttaaacg actctttttc
    20101 tttggcggtt ggatgacagt aagtaatatc ataagtccgg tcatggtgta ttttgatcga
    20161 tttatagtgt caaatattat gggggcagat aaagttgcat tttattctgc accagcggag
    20221 gttattctaa aattaggaat aatacctgca gcaatcggga gggcagtgtt tccaaggtta
    20281 agtaacatca aagactttaa agaatttaaa cgtaatgtaa ataaatcatt gcttttaatg
    20341 tttctaatct gtttgccggt gataatcata ggcttgttat attcagggct tgtattgaaa
    20401 atatggtttg gtgagaatta tcaaattaat tcctttaata tattaaatgt gttattgatc
    20461 ggttttttct tcaatgcgct ggcaatgata ccattctctg caatccaggc attaggaaaa
    20521 tctaaaatta ctgctttgat tcattgtgct gaattggttc cttatttagc ccttttgtat
    20581 tttatggtcg aaaaatatgg gttactgggt gccgcaatat cctggagcat acgtgtaatt
    20641 ttagatgctc tgttattaca atggctttat actagaatgt gttctgtata tgaaaactaa
    20701 aattaaccaa ttactgagtc acttggataa tgaatagtaa tatttacgct gtcattgtga
    20761 catataatcc cgaacttaaa aatctgaatg cactgatcac tgaactaaag gagcaaaatt
    20821 gttacgttgt agtagtcgat aataggacta atttcacctt gaaagataaa ttagcagaca
    20881 ttgaaaaagt tcatttaatt tgtcttggta ggaatgaagg tatagcaaaa gcacaaaata
    20941 ttgggattcg atattctctt gaaaaagggg ca
//
LOCUS       DAJTSI010000067        20777 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000067 DAJTSI010000000
VERSION     DAJTSI010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20777
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.067"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..579
                     /locus_tag="OO327_002194"
     CDS             <1..579
                     /locus_tag="OO327_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6018731.1"
                     /translation="GYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAM
                     ASQRAKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKML
                     FSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVS
                     LPDFSHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="OO327_002195"
     CDS             597..1235
                     /locus_tag="OO327_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6018732.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="OO327_002196"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="OO327_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR6018733.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHNELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="OO327_002197"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="OO327_002197"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR6018734.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="OO327_002198"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="OO327_002198"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR6018735.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="OO327_002199"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="OO327_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR6018736.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="OO327_002200"
     CDS             complement(5552..6613)
                     /locus_tag="OO327_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR6018737.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="OO327_002201"
     CDS             complement(6610..8007)
                     /locus_tag="OO327_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR6018738.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDALLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGALLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="OO327_002202"
     CDS             8163..9161
                     /locus_tag="OO327_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6018739.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="OO327_002203"
                     /pseudo
     CDS             complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="OO327_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10217..11298)
                     /locus_tag="OO327_002204"
                     /pseudo
     CDS             complement(10217..11298)
                     /locus_tag="OO327_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="OO327_002205"
     CDS             complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="OO327_002205"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR6018740.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="OO327_002206"
     CDS             complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="OO327_002206"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCR6018741.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14232..14855)
                     /locus_tag="OO327_002207"
     CDS             complement(14232..14855)
                     /locus_tag="OO327_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6018742.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(14852..15274)
                     /locus_tag="OO327_002208"
     CDS             complement(14852..15274)
                     /locus_tag="OO327_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6018743.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSHLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(15296..16071)
                     /locus_tag="OO327_002209"
                     /pseudo
     CDS             complement(15296..16071)
                     /locus_tag="OO327_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(16089..17141)
                     /locus_tag="OO327_002210"
     CDS             complement(16089..17141)
                     /locus_tag="OO327_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR6018744.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17172..18014)
                     /locus_tag="OO327_002211"
     CDS             complement(17172..18014)
                     /locus_tag="OO327_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR6018745.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(18001..18882)
                     /locus_tag="OO327_002212"
     CDS             complement(18001..18882)
                     /locus_tag="OO327_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR6018746.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18903..20186)
                     /locus_tag="OO327_002213"
                     /pseudo
     CDS             complement(18903..20186)
                     /locus_tag="OO327_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(20200..>20777)
                     /gene="ycjM"
                     /locus_tag="OO327_002214"
     CDS             complement(20200..>20777)
                     /gene="ycjM"
                     /locus_tag="OO327_002214"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCR6018747.1"
                     /translation="NWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAIL
                     LSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAVY
                     HELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHVN
                     ITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5070 a         5667 c         5053 g         4987 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgagcaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc tcacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca acgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcgacttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagagtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtaccg
     5701 tcagaagtcc agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaagcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cctttcaaac gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcatc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgcgccagtt tggactcgtc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagacggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gcatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag caaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actctagcga tccagcccaa
     9481 tgcgcgtata cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc gcgattttcc
     9781 agcgctgcat attcgccgtg tctttgcccg gttcatcaac gtagtgataa ccataattac
     9841 ggaaaccgcc ggtcaggccg aaactgaaat catcgctttc gaggaactga taatgcacct
     9901 ccagctccgg gcgatcaaac ctcgttccac gtttacccgc actataatct accggccctt
     9961 cctgataata ggccagagca attctccacg gcccgttggc ggcattaaaa tagactgaag
    10021 gctccgccag cccatccata tcttcgccat aaccctcgac gttttctatt tcgtacatcg
    10081 caccgatatt aaagtgccag tcgctctttt cctcggcctg tgcgcaggcc attcccgcac
    10141 acatcaccag tgcggtacag ggtaataact ttttttgttc cttgtattat cctgggggaa
    10201 tgccttcccc ctgtatttag cgaattgcta tttccgtttc tgcatcaaag aaatgacatt
    10261 tcgtcatatc aaaatgaata gtgatatttt ctcctgcatg ataatcattt aacgcaccag
    10321 cacggaccac taactcgtgc ccccaaccgt ggtgtagagc ataaattccg caccggttaa
    10381 ttctgccacg ctaattttgg cggaaatgtt attttcctct tgcgcgtccg gatgaatatc
    10441 ttccgggcgt attcccatca cgatgggctt atgcaaactt tcctgtgttt tcagaaccgc
    10501 taatttctct tcgagaatgg ttaatttaag cgtttccgta acgaatttat cgccatcgat
    10561 cgtgccgcga ataaaattca tcgctggtga tccaataaat ccggcaacaa acatattcgc
    10621 gggttggtta taaacggttt tcggcgcacc tacttgctga acaatcccgt ctttcataat
    10681 cacaatccgc gccgccatgg tcatcgcttc ggtctgatcg tgggtcacgt agatcatggt
    10741 ggtgttcagt ttctgatgca gcttgctgat ctctgcacgc atttgtacgc gcagcttggc
    10801 atcaaggtta gagagcggtt catccattaa aaacacgccc gcttcgcgca caatcgcccg
    10861 cccgagcgcc acacgctggc gttgcccgcc ggaaagcgcg cccggcttgc gtttcaggta
    10921 ctcacgcagg ccgagaattt gcgccgccca gttcacccgc tcatcaatca cctctttggc
    10981 gattttttgc atcttcaggc caaacgccat gttgtcgtaa accgtcatat gcggatacaa
    11041 cgcgtagttc tggaacacca tcgcgatatt gcgtgctttg gctggaacgt cattcattcg
    11101 tttgccgtcg atcaacagat cgccgccgct gatctcctca agcccggcaa tcatgcgcag
    11161 ggtggtcgat ttaccgcagc ccgacgggcc gacaaatacg atgaactctt tatcggcaat
    11221 ttccaggttg aagtccttca ccacatgcac ctggttatcg tagatttttt gaatatgttg
    11281 taacgaaagc tgagccatgt tgattccttt gctatacgtt ttgccagaag gccgataacc
    11341 gcgaccaggt cagggagtcc gttgagggca acagtaattg cgccccggtt aagcccgcac
    11401 cgatccccac cgagcgcata ccgctggcgt taatggcgtc aatgcccgcc tgcgcatctt
    11461 caatgccgat acatgcctgc ggcggcacgc ccagcccggc acaggcagcg agaaagattt
    11521 ccggatccgg tttcgagttt ttaagttggg aagcatccgc gcagaaggtg aaaaactcac
    11581 gcagctccag cgccgctaaa atcgtcggcg cattcaggga gacggaagcc agccctaccg
    11641 gaatctgctg tgcacggagc tctgccagca aattgcgaat accgggcagc acagcattaa
    11701 ccgtcagctc gcgcagtgag tggacgtaaa gcagattttt gcgatacgcc agttgcgccc
    11761 gctcctgcgg gttaaagtcg ccctctttgc ccccgtgttg cagaatgcgc cgcagagact
    11821 catcgcggct gatccctttt agagattcgt taaactgcgc atcaatactg atgccaattt
    11881 cagcggcaat ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg attacaccat
    11941 ccagatcgaa aattacccct tgcagtttca ttcatcctcc tgatgcgtgg tagcggtccc
    12001 attgaagggc aaaataaaat cacccaaaca gaaaacagat tcttcagcca cggatataag
    12061 ctgaccgtcc aaacgcagtg aaacgggcgc agaagttcga atcgcaatac gctgcgcgtc
    12121 gagagtgacc tgtaattcgc agccctgcca gaacagaggg aaagacaact gttgccactg
    12181 ctcaagtaac gccggattta gatgcaattc accgtcacgc acgctcaccc cggcaaaacc
    12241 ctgaatcgcc cccagccaga tagcgccagt tgcggcagca tggataccat catcacaact
    12301 atgcggatca gcaccaagat cgatttcagt cccctcgcgc cagaactgat aactttgggt
    12361 cagcaggcca cagcgtgcag caacaatgcc gtggattgct ttacttaatg acgagtcgtg
    12421 aatagtgcgc ggttcataaa attgcagatt ggcaagacac gatgccgatg agaactgctc
    12481 tggcagcatg taattgagca tcaccacatc agcttgtttg aggatctgca tctcgttcac
    12541 ttctgcgcgt gaataatcca gcagaatggt ttgcttcccc gccgccgctt tgtatttcgc
    12601 cagattaatc gccggcttag tcataaacga atcatcctgc ggcaaaacac cgtcgggctg
    12661 aatttctggc agccgcagtt ctttaaggaa catttcggca cgatgaataa acgcgtcgtc
    12721 actacagcca aactggcggg caatactcag cgcctgctgg acgttgtaat acaccatata
    12781 gctggtgaag gcgttattat tgacatgttc ggtatattcg tctggcccaa taacatcatg
    12841 aatttccaga cggtcgttaa ccctcaccgc gcggctaatc cagaactttg cagtttccag
    12901 aagtagcgcc atgccttcat gcgcaatgaa actttcatcc cccgtggtct gccagtattg
    12961 aataaccgcc caggcgatat cggccaccag atgatgttcc gcctgcgccg aggccacttt
    13021 ttgccgcagc ccggtgcgaa tgttaatggc ggcaaattcc ggcgtctctt cttcgccgct
    13081 gcgcgcgctt tcccacggaa atagcgcgcc ctgccagccg ttgcgtcgcg ctttctcctg
    13141 cgcgcctggc aagttgtgcc agcgataacg cagtaaactt cgggcaaccg tcggatcgct
    13201 aaacagatgg aacggcagca aaaatacttc tgtatcccag aaaacgtggc ctttgtagcc
    13261 ttcgccggtt aagccttttg ccgcaatact gctgcgctcg ccgtgagccg gcgtcatgat
    13321 gcgcagatga taaagcgcat aatctaacgc ttgctgatcg tgcgcatcgc cgccatttac
    13381 cgtgatacgg cgtttctgcc accattgacg ccagttttct gttgatgctg caagatgttg
    13441 gtcgtaactc tgttgcgcgc acatttcaag ctggcgaagc gacacgctgc cccactcgtc
    13501 taaagcagct tgcctgtcat cccgccagtc gatccacacc agtttttgca acgtcactgt
    13561 ctcgcctgca tgaagctgcg tactggtatg ttgcagtaaa cggcgctctt tggcggtata
    13621 gcattgctgc acatcaccgc tcacctggca gcaacaactg atggccacat cactgcgccc
    13681 atcctgggtg gtgtagatcc cctgcatcaa atgctgacca aacacccgca cctgggtttc
    13741 gtcgagatgc tggcgaccgt ggttggtttg cgtggcgtca atgcctgttg aaatcagcac
    13801 tgacgcgtcg gcgtccagtg gcataatagt gatttccagc gcaatgagcg gcagttggtc
    13861 tgccgaaaca aagcgacggc tggcgatggt gtaacctgcg ccgttgctgg tacgccagac
    13921 aacgctacgg cgtaattctc cactggcaaa gtcaagttca cgctgccagg cttcgcggga
    13981 taacgagaaa acctcaccat ttatggcaat ctccatcccc aagatatcag gcagattcac
    14041 cagttcgttg atttcacctt ttcccgcccg atgatacagc cccgccagat acatccctcg
    14101 cgtctggcgc gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc cgttcccctg
    14161 cgccatcagc gatgcatact tgttcagggt atgctggctg aaatggggtt ctgataacgt
    14221 tactggcctg gtcataattc cacacgcgtc cctgtttctg cggattgata taacgccgcc
    14281 accagttgct ggatgatgta cccctgctcg gcgtcggcta tcatcacggg cttgccctgt
    14341 acgtgattga taaaggcttc catgctgcgc aaatggcggt tgtcgtctgc catttcccgt
    14401 tgcatcagcg tcattaattc accgttgtta tcggtgtaga tatgtgctgg aaacagcgtc
    14461 gcaccggctt tatcaccaca aaagctgacg ttcataatca actgttcgcg gatgttgagt
    14521 gcaaatgacg tttccagcca cagaatgccg ccgttatgaa attcaatggt gccaaacagc
    14581 gaatcttcga cgctgtaagt tgccggatcc cactcgccaa attgaccaca gctcttttgc
    14641 gtgccgatct tttgaaaact atgcgcattc acgcttttca ccgccggaaa gcccagcaca
    14701 tacatcgcag catccagcat atgaatgccg atgtcgatca gcaggccacc accctgcagt
    14761 tctttattgg taaagacacc ccagccggga acgccgcagc gacgcagggc gcgggcggtg
    14821 gtaacgtaaa tctctcccaa aacgccgttg gtcacctgtt cacgcagctg ttgcgtatcg
    14881 agcgcaaacg atggtgaaag tcgtaggcca gcaccttgcc cagtttgcgc gcggtatcgc
    14941 acatttccca cgcctgttct gtcgtcatgg cgggcggttt ttcgcacatc acatggcagc
    15001 ctgcttccag tgccatcagg gtatgttcgt aatgaaaacg gttaggtgag ccgacgctaa
    15061 ccacatcagg tttcaccgcc agcagcatgg cctgcggatc gtcccacacg gatgcattcc
    15121 cgtatttttc tgccagcgcc tgcgcctggg aaaggtggct gtcacagaca gccaccagtt
    15181 ccagatcgtt gcgggtgcag tagtacgaag catgaacctt atccgccacc tggcccgcgc
    15241 ctattatcgc gacccgcagc ggagagcttg tcattgcact tttcacaaag acctcttagc
    15301 aggtacgcaa ccaggccaac gaatcacggt acgcctgggc aggatcttct gcccggatac
    15361 gcccttcata caccacataa ccctgatagt tatcggcgcg cagctgttca aacagcgcgt
    15421 ggaaatcgag ggtgccactg cccggctggt aacgatggtt atcggcaata tgcacatgac
    15481 cgagcaagtc acggttgtcg tgcagcgcct gcgccaggtt atcttcttcg atgttcatgt
    15541 gatagaaatc gccgataatc tgcacatgtt taagattgtt ttcgacgatg taacgacgag
    15601 catcggcgag ggtgttgatc atatgatcct gatagcggtt taacggttcg agatacacca
    15661 cggttccggt acgcgcggcg acctgttcca gtacgcgcag ggaatcactc accagtttgc
    15721 ggtcgccgtc caggctacgc ggcgaggtca tcggcggtaa gcggaaggta aacatgcccc
    15781 acgctgccgg aaccccccca cttctgccag cgcctcgaga atgcgttcga tctgctttaa
    15841 gccattaaga cgacgctctt cgataaagtc accaatccag ccgtcatagc cgccgcaggc
    15901 ggtggtcact ggtaaaccgg tttctttgat cgccgctttg acttcttcga tgttgttgac
    15961 cagcagtttg ccgtcaatct caaaaccatc gaagcccatc tctttgatat aacgaaatct
    16021 ctcgcggata ttttccggaa agaatgcctg attctgtgtg ccgattttca ttttttcatt
    16081 cctttgaatt aaaacgtaac gcccattttg atgctctgtt ccggatgctg atcgacatac
    16141 tgcatgtagc tttccgggct gttggcaaag gtcactaccg ggtcgattaa atcttcgcaa
    16201 ttgagataac cgttcatcag cagttcccag caggtttctt caatacgctt acggctccag
    16261 cgcggataat ccgggttcgg ttcgctgcac gcgcgggaga aaacaatttt ggcgttattg
    16321 aaatgcgctt cgcgtccgag gttaaaacct tcagcaaatg gtttggcaaa cgcgacatag
    16381 gagatggtgc cgccgtaggc caggccgcgc agcgccgatt gcagcgcgtc ggcgtaaccg
    16441 ctggtttcga taatcacatc agccccctgc ttgccggtca gcgttttgat ctctttgcct
    16501 acgtcagtgc caatggggtt aaggcagaaa tcagcaccgt ggcgacgggc aatatcacag
    16561 cgatgggcga taggatcgac gccaattacc accgatgcgc cagcgcgttt cgccagttgg
    16621 atggcaattt gaccgatcgc gccaagccct accaccacga caaaatcccc tacgcgtacg
    16681 ttggcatcgc gcacgccgct catggcaaac tgcgccgggt cgtaacagac ggcgtttttc
    16741 caggagctgc cttgcggcat tttgcgcagc ttgtagttat tcactgcgtt aatgatgacc
    16801 gtctcggaga gcgggccgta gccgcatacg ctgtcgccca ccgcgtagtc tgtaacgtcg
    16861 ctgccgcact cgataatgtc gccaaccacc atgttgccaa gctggaattt gccaaactca
    16921 atgccgcgcg gcgcatctgc cggacgcggg gtgaacatct gccactcgcc gttaaagtct
    16981 tcatcgataa acgggctggc ggcgcggaag tcgaccactt ccgttccgtg tttcggtgcg
    17041 ccgaaacgca cgcggatctt cacttcattg gctaaaatcg cccgatcttc atactcaacc
    17101 agcgcagcaa cacgcggtgc tgtggctact aactttttca tgtaaaactc cttgtaaaga
    17161 aactggccgc tttagccctt cacgccaccg gcggtcaaac cactcttaat aaaacgttcc
    17221 aaaagcgcat acataatcac caccggcaat gcggtcacca gcgaggccgc catcatccgt
    17281 ccccagatgt aatcaggcgt actgaacagc gcgttcaggc ccactggtaa ggtgaaattg
    17341 ctggcgctgg agaggaaaat cgacgcaaac agatagtcgt tccacgccac cataaagcag
    17401 tagacgaaca ccgatatcag cccggacatc gccaggggca cggtgatacg gaagatgatt
    17461 tgcagacggt tgaggccatc catcatcgcc gcttcttcga tctcatcagg gatggtgtcg
    17521 aagtaacttt tcagcataaa cacggcggtg ggcagggttt gcgtcaccat ggtaatgatc
    17581 agcgccatct cggtgtcgta aataccaagc gcggtgatga ttttgaacag aggcaccacc
    17641 agcaaaatgc cagagaacat atacaccgtg taaaagctgg cgttgatggt catccggcct
    17701 ttaaagcgca ggcgggaaag cgcgtaagct ccgagaatgc cgagaaatac cgccaccacg
    17761 gaggaaacca ccgacaccac catgctgtta cggaagtagt cgacaaacgg gaaaatcatc
    17821 gggttaaaaa tgtcgacgta atgctccagc gtccactgtt gcggcagcag cgtaggatgc
    17881 agcgatatcg cctctttcgc gcttttgaac gaggtcatca gcatcacaaa aaacggaaac
    17941 agcgtgatga tgagaaacag cgccagcccg cagtaaaaac cgatacgact gagcgtgcgt
    18001 ttatttgttg ccattgaggt tcacccgttt cctggtcagc agaatgacag cgaaaataat
    18061 gatgaagagc accaccgaga tcgccgccgc tttccctaag tcattaaagg caaaggcggt
    18121 tttgtagaga tagacaccaa gaatatcgac cttggttgtc agcagataaa catcagcgaa
    18181 catgtagaac atccagatgg tgcgcagggt caccaccgtc gccaggaccg gcataattgc
    18241 gggcagcgtg acgatgcgaa accgctgcca ggcattagcg ccatccatct ctgccgcttc
    18301 gtacagcgat ttgtcgatgg tctgcaaaat cgccagaaac gagataaagg catacgggaa
    18361 gtagcgccag atggcgaaca gcaccaccag cacaaagcta ctgcccggat tatcaaacca
    18421 cagcggtgcc tgctcataga gatgcagaag atcgacgccg aggtagttca caatgccgta
    18481 gccgttgttg aacatgtatt tccaggcaaa caccagcgaa atggacggcg ttacgtagga
    18541 gaggatcacc agcgaacgcg cggttttgcg caggcggaac tcgcggttga aaaacatcgc
    18601 cacggccagc ccgagcacgg tgctgcccgc caccaccagc gccgtatacc agaccgtcat
    18661 ccacagcgag tgccagaagc cgggatcgga gagaatgcgc acatagttac tcaccccaac
    18721 aaacgttgac tcgatgttgg gattgagcgg cagacgtaaa aaactgattt cgatattcga
    18781 caccatcggc cacgccacca gaccgcccag cagtaataag ctgggcgcga gaagcagcag
    18841 cgcaaacggc atatcggaac gacctgaaaa aagcctgttc attgcggatt cctgtcctgg
    18901 gtttagtgct gttcgatcag ttcatccagc tttttctggc tcgcttgcag cgtgccaggg
    18961 agatcggctt tacccacggt gacgttatgc accatggagc tcaccacacc agaacccgtc
    19021 acatcgccca tgcgggtaaa gtttttatcc cctactgcgc caaagacctg aatatttggc
    19081 agttcaccga ttagctgatt cggtagttca cccagcgcct taataacgtc gttgtctttc
    19141 caggtggcgg tagtgacaac cgctttattc accggcagcg cagcacctgg cgacatcatc
    19201 acccagtcgg caatgttgtc cgcctgctcc ataaaggtga caaatttttc tgctgcttcg
    19261 gtcttttgtc cggcggtaat ggtcagcgag gtcaacatgc cgtagaccgc agagtttttc
    19321 tcggttggca ccacgaaacc gacgtttttc gggtcgcctt ctttaatcac agccggaagg
    19381 atataggtgg agtaaatcgc catcggcgcg gtgccgttca taaaggcgtc tttcacttcc
    19441 atgatgtcgt tagaacccgg catggtgttg gcagcaaggt cgcggtaata ggtcagcgcc
    19501 tgcatcatct ctggtgtatc aagggtgatt ttgccttcgg cgttaaagac gttggcctag
    19561 ttggataacg caaactggga gaaggattgt tccgtcagca cgctttctgc cgtaggcagc
    19621 gcaataccgt attttttatt cgccgggtca ttcagtttct gtgcaacgtc cagcagctgt
    19681 tgccagtttt tcggctcctc cagccccgct tttgccagca catctttgcg ataccagatg
    19741 ccgccaatcc aggcgctgac aggaacaccg gtccatgcgc taccatcttc ggtacgcaca
    19801 atacgcagta cgccatcgta aaacgcgcct tcaccaacgt tgctgatgac cgtggcaacc
    19861 gctttgcgat cgataagctg ctctttatcc ataactttgg cgtagtcatg gctggtttcg
    19921 atcacttccg gcagcgagcc gctacgtgaa agggtgatga ctttggtgtt atgagcgtct
    19981 tcttccacgg gcacttgttt aacggtgata cctgggtttt ctttttcaaa acgggcaatc
    20041 aatttgctga taactgcctg acgctcctgc tccaccgaag aatgcataaa ttcgatggat
    20101 acatgggttt tattttcttc tttacagcct gaaatcaggg cgcaggaaac cagtgctgat
    20161 aacagcacga tttttgattt aatcatgaga tgttcctttt tatttaatcc acataacctg
    20221 ccaggggcgc agcgttattt cattacccaa tatatcaact tcactaatca gatcccgacc
    20281 atgcagatca gtaatattta catgctgaat atttttactg acattaaaca atccagtcag
    20341 acaattacca tcagcgttac ttctttgaat acgcattacg gatgaattaa ccgtatcgat
    20401 ggtaaaatta ttatccggat gaaactcgtt atggctgcga cgaagtgtaa ttaaacgcga
    20461 caactcatga tataccgcat gccttaatgt agcttcatcg ttcagttctc gtgttatctc
    20521 ttcactgtga tattttttac ggttaatcgc acggttatat ccgagttttt cgacacctgc
    20581 gtaatcatta cgcgagccaa gaatactttg aatatatatc gcgggtacac cggggaaact
    20641 taacaaaatc gcatgggcaa ggataaacct ggtgcaacgt tcttcatcgc tactctcgcg
    20701 gcggcttaac gcattcatat aggtgacatt catctcatag ggactgcgcg tgccgtcggg
    20761 attatttttc cagttta
//
LOCUS       DAJTSI010000068        20691 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000068 DAJTSI010000000
VERSION     DAJTSI010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20691
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.068"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            81..350
                     /locus_tag="OO327_002215"
     CDS             81..350
                     /locus_tag="OO327_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCR6018748.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            344..709
                     /locus_tag="OO327_002216"
     CDS             344..709
                     /locus_tag="OO327_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR6018749.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(710..1498)
                     /gene="bioH"
                     /locus_tag="OO327_002217"
     CDS             complement(710..1498)
                     /gene="bioH"
                     /locus_tag="OO327_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR6018750.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1536..2219
                     /gene="gntX"
                     /locus_tag="OO327_002218"
     CDS             1536..2219
                     /gene="gntX"
                     /locus_tag="OO327_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR6018751.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2278..2853
                     /gene="nfuA"
                     /locus_tag="OO327_002219"
     CDS             2278..2853
                     /gene="nfuA"
                     /locus_tag="OO327_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR6018752.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3214..4530
                     /gene="gntT"
                     /locus_tag="OO327_002220"
     CDS             3214..4530
                     /gene="gntT"
                     /locus_tag="OO327_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6018753.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="OO327_002221"
     CDS             complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="OO327_002221"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR6018754.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6669..9062)
                     /gene="malP"
                     /locus_tag="OO327_002222"
     CDS             complement(6669..9062)
                     /gene="malP"
                     /locus_tag="OO327_002222"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR6018755.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9674..12379
                     /gene="malT"
                     /locus_tag="OO327_002223"
                     /pseudo
     CDS             9674..12379
                     /gene="malT"
                     /locus_tag="OO327_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="OO327_002224"
     CDS             complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="OO327_002224"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCR6018756.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="OO327_002225"
     CDS             complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="OO327_002225"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR6018757.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14849..16447
                     /gene="rtcR"
                     /locus_tag="OO327_002226"
     CDS             14849..16447
                     /gene="rtcR"
                     /locus_tag="OO327_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCR6018758.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16429..17187)
                     /locus_tag="OO327_002227"
     CDS             complement(16429..17187)
                     /locus_tag="OO327_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR6018759.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17204..18034)
                     /gene="glpG"
                     /locus_tag="OO327_002228"
     CDS             complement(17204..18034)
                     /gene="glpG"
                     /locus_tag="OO327_002228"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR6018760.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18079..18405)
                     /gene="glpE"
                     /locus_tag="OO327_002229"
     CDS             complement(18079..18405)
                     /gene="glpE"
                     /locus_tag="OO327_002229"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR6018761.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18595..20100
                     /gene="glpD"
                     /locus_tag="OO327_002230"
     CDS             18595..20100
                     /gene="glpD"
                     /locus_tag="OO327_002230"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR6018762.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20369..20599)
                     /locus_tag="OO327_002231"
                     /pseudo
     CDS             complement(20369..20599)
                     /locus_tag="OO327_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         4889 a         5693 c         5217 g         4892 t
ORIGIN      
        1 gtcagtagat actctaccct gcttaagtgc tactttgtgc tatcttgcat gtaacacaaa
       61 gtagcactta gggaactgat atgcagacag tgaaattacg gcaacaagga ggagccatga
      121 tcgttactat cccacgagat cttgccatcg atcttggctg gtcaccgggc acagagctta
      181 ccgtggagaa aaaaggcgat agcgttaatt tgcgcgccac agagcacaaa ccgcgtgggc
      241 gtcttaccgt cgcgcagttg ttgagccaaa tcgatgaaag cgaaattacg gagctaaatc
      301 agtccacaga aggatgggct gaagggaaaa aaggtaatga agcatggtaa aggcacggac
      361 gccacatcgt ggtgagatct ggtattttaa ccctgatccg gttgccgggc atgaacttca
      421 ggggccacat tattgcattg tggtaacgga caaaaaactc aacaatgttt taaaagttgc
      481 tatgtgctgc ccgatttcaa caggggcaaa tgcagcacgt tccacagggg tgacggtgaa
      541 cgtcctcccc cgtgatacgc aaaccggtaa cctgcatggc gttgtacttt gtcaccagct
      601 aaaagccgtc gatcttattg cccgtggcgc taaatttcat accgttgccg atgaaaaatt
      661 gattagtgaa gttatcagta aactggtgaa tttaatcgac ccacaataat tagctctcac
      721 ttaccaccaa caccctctgc ttcaacgcca ccagcaggtg acaaaactcg accggatgcg
      781 aaataaacgg cgcatgggcc gctttggcga agatatatga ttcgctgtga ggccaaagtt
      841 tatccagcat cggcaccact ttgcgcggca ccagaccgtc gagatagcca tacaatcgca
      901 aaaacggcat ggacacgttt tgcagcggct gacggagatc gaccgttttc aggatttcca
      961 gcccgccatt aagcacgtca acctccggca tcggtaacgc cagaacggtt ttcttcaacg
     1021 cccgcgcatc ctggcgcgcc gtttcagtcc ccatggtttg taacgccagg aaccgctcca
     1081 ctgtacgctg aaaatcatca ctgagttgct gctgaaatcc cgccagcacg tccggtttta
     1141 tccccggcca ctcgtcacga gcactaaaac aaggtgacga cgccacggtg accagcgcct
     1201 gaacacgctc gggatgggtt aacgcaatct ggcttgccac cagcccgccc agactccagc
     1261 ctaaccaaat ggctttatca ggtgcctgtt gcagcacggc ttcggccata tcagcaagtg
     1321 acagcgcacc aaatccccgg ctacgcccga agccgggcag gtcaacaagg tgcagcgtaa
     1381 aatgcgagct aagttcctcg tcaatgcaac gccacacttc ggcattcagt ccccatccgt
     1441 gcagcagcac aagatgaaca ttcccctgac ctttggtctg ccaccagatg ttattcatcc
     1501 gctattgttc tcttttgact tacaaggatg aacatatgct aacagtaccg ggattatgct
     1561 ggctatgccg aatgccactg gcgttaggtc attgggggat ttgttcggtc tgttcacgcg
     1621 ccacccgcac agataaaacg ttatgcccac aatgtggatt acccgccaca cactcccatc
     1681 ttccctgcgg tcgctgcctg caaaaaccgc cgccctggca aagactggtc acggttgccg
     1741 actatgcgcc gccgttaagt ccgcttatcc accagcttaa attttcccgg cgcagtgaaa
     1801 tcgccagcgc cctgtcacgt ctgttactat tggaagtctt acacgctcgt cgcaccaccg
     1861 ggttgcaatt gccggatcgc atcatcagcg ttccgttatg gcagcggcgt cactggcgtc
     1921 ggggatttaa tcagagcgat ttgctgtgtc agccgttatc acgctggttg cactgccaat
     1981 gggatagcga agccgtcaca cgtacacggg ccactgcgac ccagcatttt ctcagtgccc
     2041 ggctgcgcaa gcgcaacctg aaaaatgcct ttcgtcttga attgcccgtg caaggtcgcc
     2101 atatggtgat tgtggatgat gtcgttacca ccggaagtac cgtcgcagag attgcgcagt
     2161 tgcttttacg caatggtgcg gcgactgtcc aggtctggtg cctttgtcga accttgtaga
     2221 gcctcgatga tgggcgtatt ataaccaact aaaatagtca actattaggc cattactatg
     2281 atccgtattt ccgatgctgc acaagcgcac tttgccaaac tgctggcaaa tcaggaagaa
     2341 gggacacaaa tccgcgtatt tgtgattaac cctggcacgc ctaacgctga atgtggtgtt
     2401 tcttattgtc cgccggatgc cgtggaagcc accgacactg ccctgaaatt tgacctgctg
     2461 accgcgtatg ttgatgagtt aagcgcacca tacctggaag atgcagagat cgactttgtt
     2521 accgaccagt tgggttctca gttaacgctg aaagccccga acgccaaaat gcgtaaagtg
     2581 gcagacgatg caccgctgat ggagcgcgtg gagtatatgc tgcagtcgca gatcaaccca
     2641 cagcttgctg gtcacggtgg tcgcgtttcg ctgatggaaa tcaccgaaga cggttacgcc
     2701 attctgcaat ttggcggcgg ctgtaacggt tgttccatgg tcgatgtgac gctgaaagaa
     2761 gggatcgaga agcagctgct gaacgaattc ccggagctga aaggtgtgcg cgatctcacc
     2821 gaacaccagc gcggcgaaca ctcctactac taagttatcc cctcttttgt agattgcccg
     2881 atgcgacgct aaagcgtcat ttcgggccta cgttgaacat gtgccagacg ttggcacagc
     2941 attgctttgg caaatccctc ccttcgtttc cccgcgataa tatgaccaac ctctcataat
     3001 ttaaatttac cccgctctgg tgattctcaa acgccaaatg ttacccgtat cattcacatg
     3061 ggtaccaaac atactcctga catctgacta caataattag ttttagtggg tatcagtcgt
     3121 ggtgccgcaa tatctctgtt cccgattggg ataattagag tttgtcgtca gaaaattgac
     3181 gttacccata acaaatgaaa ggccaggtaa atcatgccat tagtcattgt tgctatcggt
     3241 gtaatcttgt tgttgctcct gatgatccgc ttcaaaatga acggcttcat cgctctcgtc
     3301 ctcgtggcgc ttgctgttgg attaatgcaa ggaatgccgc tggataaagt tattggctcc
     3361 atcaaagccg gtgtcggcgg gacgctcggt agccttgccc tgatcatggg ttttggcgca
     3421 atgctgggca aaatgctggc agactgcggt ggcgcacaac gtatcgccac cacgctgatt
     3481 gccaaatttg gtaaaaagca catccagtgg gcggtggtgt taaccggttt taccgttggt
     3541 tttgccctgt tctatgaagt gggcttcgtg ctgatgctgc cgctggtgtt taccatcgcg
     3601 gcttccgcga atattccatt gctgtatgtt ggtgtaccaa tggcggctgc actgtctgtg
     3661 acccacggct tcctgccacc gcatccgggc ccgactgcga ttgccaccat tttcaatgcc
     3721 gatatgggta aaaccctgct gtacggtact attctggcaa tcccgaccgt gattctcgcc
     3781 ggtccggttt acgctcgcgt gttgaaaggt atcgataagc caatcccgga aggtctctac
     3841 agcgcgaaaa ccttcagcga agaagagatg ccgagctttg gcgtcagcgt ctggacctct
     3901 ctggtgccgg ttgtgctgat ggcgatgcgt gcgattgccg aaatgatcct gccgaaaggt
     3961 cacgctttcc tgccggtagc ggagttcctc ggtgacccgg taatggcaac gctgattgcc
     4021 gtgctgattg cgatgttcac ctttggtctg aaccgtggtc gttcaatgga tcagattaac
     4081 gacacgctgg tttcttccat caaaatcatt gcgatgatgc tgttgatcat cggtggtggc
     4141 ggtgcgttca agcaggtgct ggtagacagc ggcgtggaca aatacattgc ttccatgatg
     4201 cacgaaacca acatttctcc gctgctgatg gcctggtcga ttgctgccgt actgcgtatc
     4261 gcgctgggtt ccgcaaccgt tgcggcaatc actgcgggtg gtatcgcggc accgctgatt
     4321 gcaacgacgg gtgttagccc agagctgatg gttattgcgg tgggttccgg tagtgtgatt
     4381 ttctctcatg tgaacgatcc gggcttctgg ctgttcaaag agtactttaa cctgactatc
     4441 ggcgagacca tcaaatcctg gtcgatgctg gaaaccatta tctcggtgtg cggtctggta
     4501 ggctgtctgc tgctgaatat ggtgatttga taacactgac tgccggatgc atcgcatccg
     4561 acatcaaccg cactctactt cttcttcgct gcagctctgc gccgtctgtc caaatccttc
     4621 agcaacttgt tcacgccatc atcggcaaac atcgactcaa gcgttgcgga aagcttgcgt
     4681 cgccagtttt tatactggta actggtgcca ggaatattca ccggttcggc catatccagc
     4741 cagtcttccg gctgtagtcc taacagagca ctgttactgt cggcaatgta gcgctgcaaa
     4801 ccacggttca gcgtcggcgt catcgacatc aacgatgcct tatgcccggc acgttttggc
     4861 agacaaccat atttatgcag tgcatccagc agcccttgct tcgccagttc gcgatcctga
     4921 tacagaccgc gcagtaccac ttcatccgga tacagcccca gggttttgcc cagcgttaga
     4981 tccccgctct cccagtaacc acgcagcgtt ggcaggtcat gtgtcgccgc aaccgccatc
     5041 gactgctccg gatacgcttt cggtgcacgg aacgtcttct cgtggtcgtt ttcgaaatag
     5101 agcactttgt aagagtacac accgctgctg cgcagcttac caacaatctc taccggtacg
     5161 gtaccgagat cttcaccaat caccatacag cgatgacgtt tactttcgag tgccagaatc
     5221 gagagcagat catccaccgg atagtgaaca tacgcgccct gatctgccgt ctcgccatac
     5281 ggtatccacc acaaacgcag catcgacatc acatggtcaa ttcgtaatgc gccgcagttt
     5341 tgcatattgg cacgcaacag ctcgataaac ggttcatagg cacgcgcggt gatgatatgc
     5401 gggtccattg gcggtaatcc ccagttctgc cccaacgggc cgaggatatc cggcggcgcg
     5461 ccaaccgatg ctttcaggca atatagttca cggtcacacc aggtttccgc cccaccttcc
     5521 gctacgccaa ccgccagatc acgatacaag ccaatcggca tttcatagcc ctggcttatc
     5581 tcccagcagg cggcaaactg gctgtaagcc agccactgca accagagata aaaatcgacg
     5641 tcatcacgat gttcttcgca gaactgacgc acttctggtg aatccacgtt cttatacatc
     5701 tctggccatg caggccagcc ccagcgcatt tcgtcctctt tcacttgctg ggcatgtagc
     5761 gcatcaaagg cagcctgcca gaacaggctg tcgccctgct ctgcaacaaa ctggcgaaac
     5821 gcggccatct gctcatcatc acgttgcgcg aaacctttcc acgccattcg taatgctgtc
     5881 atttttaggg cggtaaccgt ggagtaatcg acccagtcgg catcgcgcgc ctgttgcagc
     5941 gtctgttgcg tggtcggcaa ctgccaccag gcctgagcct cttcgctaag atggaaatct
     6001 tcaacggcgt taacgtcgat ataaatcaca ttcagccaac ggcgagaaga cgggctgtat
     6061 gggctggcgc tctccggatt tgccggatag agcgcatgaa tcgggttcag gccaatgaac
     6121 gacccgccac gttttgccac atccaccagc atcgctttga gatcgccaaa atccccaata
     6181 ccccagtttt tttccgatcg cagcgtataa agctgaacgc aggcacccca cagcttttgt
     6241 ttattcagca aagcctgcgg ttcgtaacaa cgtttcgggg cgacaatcac ccggcaatgc
     6301 gcgcgctggt cgtcctgggt gagtgttagc gtgtgataac cttccggcag cttcgtcggt
     6361 agattgaacg ctttgccccc cgttacatgg cctttgtact gcgttccttc ttcggtggtc
     6421 agctgccagc tatattcgcc gctgccctcc accaccatcg gcattttttt gccgctggta
     6481 taaaccatga cattcgggac tggcgttacc gccactttcg tggcggtacg ttgatgcatc
     6541 gcgtcaagca aacgccgttt ggtttcggcg ctaatcgact gcggtttacc gtgggcattg
     6601 atgtaattgg ggctaatccc cgccgccagc gcggcattat ccagacgttt gctttccatc
     6661 gagcttcctt agcgttttgc ctgccagata cgagcctgat aatcgcgaat agagcgatcc
     6721 gagctaaaca taccgcagcg ggcggtattg aggatcgccg cgcgagtcca ggcctcctgg
     6781 tcgcggtaca gcacatccac ctgcttttgt gcctcaacat aggctgcgaa atccgccatc
     6841 accagatacg gatcgccgcc ctgtttgccg atactgtgca gcatctggtc gaaggcatgc
     6901 ttatcgccgt cgctgtattt accgctttcc agctctttca atactgcgtc cagcacctta
     6961 tctttcttcc gccatttcac cgggtcgtag cctttggcca gaatagcctt cacttgttcc
     7021 acggtatgac caaaaataaa gatattttct tcaccgactt tctcggcgat ttcaacgttc
     7081 gcaccatcca gcgtaccgac agtaagcgca ccattgagcg ccagtttcat attgccggta
     7141 ccggaagctt ctttacctgc agtcgaaatt tgttcggaga tatccgccgc cgggatcagt
     7201 ttttccgccg ccgaaacgca ataatccggc aggaacacca ccttcaactt atcgccaacc
     7261 agtggatcgt tgttgatcac gtcagccact ttgttgatcg caaagataat attcttcgcc
     7321 aggtagtagc ccggtgccgc tttcgcgccg aagaggaaga cgcgcggtac gcgatcagcc
     7381 tgcaggtttt cacgaatttc tttgtacaac gccagaatat gcagcagatt caggtgctgg
     7441 cgtttgtact catgcagacg tttgatctga atatcgaaaa tcgcctgtgg attgatctca
     7501 ataccggtac gaactttcac aaactccgcc agacagactt tattcgcctg cttgatctcg
     7561 cgatattgct gacggaattt cgcatcatca gcgaattttt ccagattgat cagctgatcg
     7621 agatcgttag cccactcttt ttgcagtgat ttatccaaca gagccgccag tgcagggttg
     7681 cactgtttga tccagcgacg tggggtaata ccgttggtaa cgttatggaa tttgttcggc
     7741 catagctggt gatattccgg gaacagatct ttcaccacca gatccgagtg cagcgccgca
     7801 acaccgttca ccgcgaaacc gccaaccaca cacaggttcg ccatatgcac ttgtttatcg
     7861 tgcaccaccg ccagtttggc ccacactttt tcatcgcccg gccaggtttt ctccaccagc
     7921 gttttaaagc gagtattaat ttcgttaata atctgcatat ggcgcggcag taagcctttc
     7981 accagtttca catcccagcg ttccagcgct tctggcatca gggtatggtt ggtgtaagcg
     8041 aaagttttgc tggtgatggc ccaggcgtca tcccagctca tctggtgctc atcggtcagc
     8101 acgcgcagca gttctggaat cgcgatagtt ggatgggtat cgttcagctg aataacttcg
     8161 taatccgcca gttcgtgcag tttacgcccc gccagatgat ggcgacgcaa aatatccgct
     8221 accgaacagg cacactggaa gtattgctgc atcaggcgca gctttttacc ggcagtatgg
     8281 ttgtcgtttg gatagagaac tttggtcagt ttttccgcat tgatgccttg ctgttcggcg
     8341 cgcaggaaat caccgtcgtt aaatttagtt agatcaaacg gatgcgcatg cgtcgcctgc
     8401 cacagacgca gcggctgcgc cacgccatta cgatagccga caacggggag atcccacgct
     8461 tgaccggtaa tggtaaactc cggctcccag cgtccgtctt tcatcacttt accgccaatc
     8521 cctacctgca catccagtgc ttcgttgtgg cggaaccacg ggtagttact gcgatgccag
     8581 tcatccggcg cttcaacctg tttgccatcg acaaaagact ggcggaacaa accatattga
     8641 tagttcagac cgtaacccgt cgcagactga ccgacagttg ccattgagtc gaggaagcac
     8701 gccgccagac gtcccagacc accgttaccc agcgccgggt cgatctcttc ttccagcagg
     8761 tcagtcaggt tgatgtcata agccttcaac gaatcctgta catcctgata ccagccgaga
     8821 ttcaacaggt tgttgcccgt cagacgacca atcaaaaact ccattgagat gtagttaaca
     8881 tgtcgctgat tcgccaccgg tttggcgaat ggctgagcac gcagcatttc ggccagtgct
     8941 tcactcactg ccagccacca ctggcgagga gtcatttcag ccgcagaatt taagccataa
     9001 cgctgccact gacgtgaaag cacttcctga aattgcttat cgttaaaaat aggttgtgac
     9061 ataggagttc cacttttctt agattttcaa cacaacgtta tcgctagttt gccaggctcg
     9121 atgttgacct tcctcatcct gcgggggatt aggcagggag gagttgcggg gatgagcaag
     9181 gaaatgtgat ctcgaccact taaagctagt gcaaaccaca ggattagcag caaatcaatg
     9241 ccatacagcg cagaaaatct gtatctaagt gcaaaaaatg gccgttgcgt attttcaaaa
     9301 agcggaaggt aactctataa attaagtaaa ggagtgaaac agtttcacaa gtaaaatatc
     9361 cagtgtgctc catctcattc ttaatagatt tattaagatc atctttttag atggcacttt
     9421 catcaggaat gaagaagaaa cccttgctta aatgaatctg atgaacataa gggaaaccag
     9481 tattcacgct ggatcagcgt cgttttaggt gagttgttaa taaagatttg gaattgtgac
     9541 acagtgcaaa ttcagacaca taaaaaaacg tcatcgcttg cattagaaag gtttctggcc
     9601 gaccttataa ccattaatta cgaagcgcaa aaaaaataat atttcctcat tttccacagt
     9661 gaagtgatta actatgctga ttccgtcaaa actaagtcgt ccggttcgac tcgaccatac
     9721 cgtggttcgt gagcgcctgc tggctaaact ttccggcgcg aacaacttcc ggctggcgct
     9781 gatcacgagt cctgcgggct acggaaagac caccctcatt tcccagtggg cggcaggcaa
     9841 aaacgatatc ggctggtact cgctggatga aggtgataac cagcaagagc gtttcgccag
     9901 ctatctcatt gccgccgtgc agcaggcaac caacggtcac tgtgcgatat gtgagacgat
     9961 ggagcaaaaa cggcaatatg ccagcctgac gtcactcttc gcccagcttt tcattgagct
    10021 ggcggaatgg catagcccac tttatctggt catcgatgac tatcatctga tcactaatcc
    10081 agtgatccac gagtcaatgc gcttctttat tcgccatcaa ccagaaaatc tcaccctggt
    10141 ggtgttgtca cgcaaccttc cgcaactggg cattgccaat ctgcgtgttc gtgaccaact
    10201 gctggaaatt ggcagtcagc aactggcatt tacccatcag gaagcgaagc agttttttga
    10261 ttgccgtctg tcatcgccga ttgaagccgc agaaagcagt cggatttgcg atgacgtttc
    10321 cggttgggcg acggcactat agctaatcgc cctctccgcc cggcagaata cccactcagc
    10381 ccataagtcg gcacgccgcc tggcgggaat caatgccagc catctttcgg attatctggt
    10441 cgatgaggtt ttggataacg tcgatctcgc aacgcgccat tttctgttga aaagcgccat
    10501 tttgcgctca atgaacgatg ccctcatcac ccgtgtgacc ggcgaagaaa acgggcaaat
    10561 gcgtctcgaa gagattgagc gtcaggggct gtttttacag cggatggatg ataccggcga
    10621 gtggttctgc tatcacccgc tgtttggcaa cttcctgcgc cagcgctgcc agtgggaact
    10681 ggcggcggag ctgccggaaa tccaccgtgc cgccgcagaa agctggatgg cccagggatt
    10741 tcccagcgaa gcgattcatc atgcgctggc ggcaggcgat gcgctgatgc tgcgcgatat
    10801 tctgcttaat cacgcctgga gtttgttcaa ccatagcgaa ctgtcgctgc tggaagagtc
    10861 gcttaaggcc ctgccgtggg acagtttgct ggaaaatccg cagttagtgt tattgcaggc
    10921 gtggctaatg caaagccaac atcgctatgg cgaagttaac accctgctag cccgtgctga
    10981 acatgaaatc aaggacatca gagaaggcac catgcatgca gaatttaacg ctctgcgcgc
    11041 ccaggtggcg attaacgatg gtaatcagga tgaagcggaa cggctggcaa aactggcact
    11101 ggaagagctg ccgccgggct ggttctatag ccgcattgtg gcaacctcgg tgctgggtga
    11161 agtgctgcac tgcaaaggcg aattgacccg ctcactggcg ctagtgcagc aaaccgaaca
    11221 gatggcacgc cagcacgacg tctggcacta cgccttatgg agtttaattc agcaaagtga
    11281 aattctgttt gcccaagggt tcctgcaaac cgcgtgggaa acgcaggaaa aagcgttcca
    11341 gctgatcaac gagcagcatc tggaacagct gccaatgcat gagtttctgg tgcgcatccg
    11401 agcacaactg ttatgggcct gggcacggct ggatgaagcc gaagcgtcgg cgcgtagcgg
    11461 gattgaagtc ttgtcgtctt atcagccaca gcaacagctt cagtgcctgg caatgttgat
    11521 tcaatgctcg ctggcccgtg gtgatttaga taacgcccgt agccagttga accgtctgga
    11581 aaacctgctg gggaatggca aatatcacag cgactggatc tctaacgcca acaaagtccg
    11641 ggtgatttac tggcaaatga ccggcgataa agccgccgct gccaactggt tgcgtcatac
    11701 ggctaaaccg gagtttgcga acaaccactt cctgcaaggt caatggcgca acattgcccg
    11761 cgcacaaatc ttgctgggcg agtttgaacc tgcggaaatt gttctcgaag aactcaatga
    11821 aaatgcccgc agtctgcggt tgatgagcga tctcaaccgt aacctgttgc tgcttaatca
    11881 actgtactgg caggccggac gtaaaagtga cgcccagcgc gtgttgctgg acgcattaaa
    11941 actggcgaat cgcaccggat ttatcagtca ttttgtcatc gaaggcgaag cgatggcgca
    12001 acaactgcgc cagctgattc aacttaatac gctgccggaa ctggaacagc atcgcgcgca
    12061 gcgtattctg cgagaaatca atcaacatca tcggcataaa ttcgcccatt tcgatgagaa
    12121 tttcgttgaa cgtctgctaa atcatccgga agtgccagaa ctaatccgca ccagcccgtt
    12181 gacccaacgt gaatggcagg tcctggggct gatctactcc ggttacagca atgaacaaat
    12241 tgcgggcgaa ctggaagtgg cggcaaccac catcaagacg catatccgca atctgtacca
    12301 aaaactcggc gtcgcccacc gtcaggctgc cgtgcagcac gcgcagaaac tgctgaagat
    12361 gatgggttat ggggtgtgag tttagccgga taatgcattg gatccggttt acatctttac
    12421 atcattcaat actcacccgc gttacgccat ctgtttctat caaaccaaac cgcaccggca
    12481 agaaacgctc caccaccgcg atattggtca gcagatggca tgaaggatgg gcgaccgtaa
    12541 attctcccgc gcccgccagc gccatcggca gtaccagttg gtcggcgaga tattccccca
    12601 ccgccgctgg gcttgccagg tagcgtttca cctctttcac taactgcgcg gctaccacct
    12661 cggcactgac gcgcttttca ccgacgacga aaaagcgttc agtgatattt tcactttcga
    12721 cttccagcga gacggtatta cccggcccct ggtcgcgcgg caggttatga atattctgtt
    12781 catgcaggga aaaaatcccc gccagtgtag cgatttcacg ctcagcaaca tggcgcggca
    12841 caccagctaa tagaacttct ccacgcatct gcacaatgtt cccgcgctcg ccaagttgca
    12901 aggtgctaaa cgatgccacg ggcgagactt ccgttgccac cacaccgcct ccggcaggat
    12961 aaaaaccgtg acgtaacagc gtggtttgct gatgaattcc tattttcgcc agcagcggct
    13021 ccagcacccg gcggataaaa tccgcaggcg gcgccgacgg gttatcggtg ccgccgctca
    13081 cttcaacacg cgaaggtcca tcggcaaacc acagcgcggg cagcaccgtt tgcagcacca
    13141 gcgtacaact tccggcgcta ccgatagcaa agcggtaatc gccgccgcgc acggtgccag
    13201 gccggaagac cagacgctgc gatcccagct ccgcgccttc caccgttgcc ctacaaattt
    13261 ccgcagccgc ttttaccgcg gtcagatgct ggcgcagcag ccccggtttc gcccgtccgg
    13321 ctcgaatgcc agtgatggta aatggctggc cggttatcat cgacaggctc agcgccgagc
    13381 gcaggatctg cccgccgcct tcgccctgtg cgccatccag cgcaatcatc cttttcatca
    13441 tttatccttt tacgcacacc acctgacgca gggtgtagat aacttccacc agatcgcttt
    13501 gtgccgccat caccgcatca atatctttat acgccatcgg gatttcgtcg atcacttcgg
    13561 catctttacg gcattccaca tgcgcggtgg cacgaatttg gtcttccacg ctgaacagtt
    13621 ttttcgcttt agttcggctc attacccgcc cggcaccgtg gctgcacgaa cagaacgact
    13681 cttcatttcc cagcccacgg acgataaagc tttttgctcc catcgaaccg ggaataattc
    13741 catattgacc agcacgcgca gacaccgcac ctttacgcgt cacgtagatc tcttcaccaa
    13801 agagctgttc tttttgcaca tagttgtggt gacagttgat ctcttccatc gccagcgttt
    13861 gtggctgtct gaccgttttc tgcgtaatgc tctgcaacgc cgttaccaca ttttccatca
    13921 tcgcatcgcg gttaaggctg gcgaaaagct gcgcccaggc cacggctctt aggtaatcat
    13981 caaagtattc cgtaccttcc ataaagtacg ccagatcacg cgacggcaac gtctcaagtt
    14041 ggtcctgcat ctctttttgc gccagatcga taaagtaagt cccgatggcg ttaccaattc
    14101 cgcgtgaacc ggagtgcagc ataatccaca cctgctccga ctcatcaagg cagatttcaa
    14161 taaagtggtt acccgttccc agcgttccca ggtgtttata gttgttagtg ttaaggaaac
    14221 gcggatattt ttgcgttaac cactgataac cggcctccag ctcagcccat ttcgcatcga
    14281 cgttaaaagg cgggttttcc catgcgcctt tatcacgttt acaacgcccg gtagtacgcc
    14341 cgtgtggtac ggccgtttca atcgcttgac gcagctccgc caggttttca ggcaggtctg
    14401 ccgccgttaa cgcggtacgc agcgcgttca ttccacagcc aatatccacg cccaccgccg
    14461 ccggaataat cgcccctttg gtcgggatca cgctaccaat ggtggaacct ttacccaggt
    14521 gtacatcagg cattaccgca atatgtttga aaataaacgg catcttcgcc gtattaataa
    14581 gttgctgacg cgcatcggct tctaccggca cgcctttggt ccacattttt accggggcat
    14641 tttcagtggt cagtaattcg taattcattt tgtttttctc ttttcgttgt ttgcggtcct
    14701 gataattgca accgtcgtgc cagaaaatta aataagcaga ttaatttatt gtttctaaaa
    14761 agattatatc tttatatccg taaacggaga tttcccgcaa agccgattta ccgataatga
    14821 aatatcgcct tttataagga tatctaagat gcgtaaaaca gtggcttttg gctttgtcgg
    14881 taccgtgctg gattatgccg gacgcggcag tcagcgctgg tcaaaatggc gtccgtcact
    14941 ctgtatatac cagcaagaat cgttggtcat cgatcgactg gaattattgc acgatgcccg
    15001 ctcgcgctcg ctatttgaaa cgcttaaacg cgatatcgcc agcgtttcgc cagaaacaga
    15061 agtggtgggc gttgagattg aactgcataa cccgtgggat ttcgaagagg tttacgcctg
    15121 cctgcatgat ttcgcccgtg gttacgagtt tcagccagaa aaagaagact atttaattca
    15181 catcaccacc ggcacccacg tcgcgcagat ttgctggttt ctgctggcgg aagcgcgtta
    15241 tctacccgcc cggttgatac aatcttcaac tccgcgcaaa aaagaacagc cgcgcggccc
    15301 aggtgaagtg acgattatcg atctcgattt aagccgttat aacgccatcg ccagccgctt
    15361 tgccgaggaa cggcagcaaa cgctcgattt cctcaaatcc ggtattgcca cgcgtaaccc
    15421 ccacttcaac cgcatgattg agcagatcga aaaagtggcg attaaatccc gcgcgccgat
    15481 tctgcttaac ggcccgaccg gcgcaggtaa gtcatttctg gcgcgacgca tcttcgagtt
    15541 aaaacaggca cggcatcagt ttagcggcgc gtttgtggaa gtgaactgcg ccaccctgcg
    15601 cggcgatacc gcgatgtcga cgctgtttgg tcatgtgaaa ggcgcgttta ccggggcgcg
    15661 ggaatctcgt gaaggattat tacgcagcgc caacggcgga atgttgtttc ttgatgagat
    15721 tggcgaactg ggcgcagacg aacaggcaat gctgctgaaa gctattgaag agaaaacctt
    15781 ttacccgttt ggcagcgatc gccaggtgag cagtgatttt cagcttatcg ccggaacggt
    15841 gcgcgatttg cgccagctgg ttgccgaagg caaattccgc gaagatctgt acgcgcggat
    15901 caatctctgg accttcaccc tgccgggtct acgccagcgc caggaagata ttgaaccgaa
    15961 cctggattat gaagtggagc gccacgccac actcactggt gacagcgtgc gttttaacac
    16021 cgaagcgcgg cgtgcctggc tggcctttgc aacatctccc caggcaacat ggcgcggtaa
    16081 ctttcgcgaa ctttctgcca gcgtcacgcg gatggccacc tttgccacta gcggacgcat
    16141 cactctggac gtggtggaag atgagataaa ccgtctgcgc tataactggc aggagagccg
    16201 cccctccgcg cttacggcgt tgctgggcgc agaggcagaa aacatcgatc tcttcgaccg
    16261 tatgcaactg gaacacgtta tcgctatctg ccgccaggca aagtcgcttt ctgctgccgg
    16321 acgacagctt tttgatgttt cgcgccaggg caaagccagc gtcaatgacg cggatcggct
    16381 acgcaaatac ctggcgcgtt ttggtctgac gtgggaagcc gtgcaggatc agcacagctc
    16441 cagttgaata tggtggtccg tcagcacctg catcacgctt actggcggcg gggcgtcggt
    16501 gtagacggca tcaaccatgc tgatgctccc catattgacc atcgcattac ggccaaattt
    16561 tgaatggtca accaccagca taacgtggcg cgagttctca ataatggcgc gcttggtacg
    16621 aacttcgtgg taatcgaact ccagcagcga gccgtcgctg tcgatgccgc tgatccccag
    16681 aatgccgaaa tcaaggcgga actgggagat aaaatcgagc gtcgcttcgc caatgatccc
    16741 gccatcgcgg ctacgtaatt cgccaccggc gagaataatg cggaaatctt cttttaccat
    16801 caacgtgtta gcaacgttga gattgttggt gacaatgcgc aaattgctgt gattgagcag
    16861 cgcgtgcgcc accgcttccg gcgtggtgcc gatatcgata aacagcgtcg agccattggg
    16921 gatttgctcc gccactttgc gggcgatgcg ctctttttct tcggtctggg tggctttacg
    16981 atcgtgccac ggcgtgttaa ccgaactgga aggcagcgcc gcgccgccat gatggcgcag
    17041 gatcaggttt tgctctgcca gctcgttgag gtcgcggcga atagtctgcg ggctgacgga
    17101 gaagtgctcc accagttcct cggtactgac ataaccctgc tgtttaacca gttcgataat
    17161 accgttgtga cgttgtgttt gtttcattta taaatccctg gaattatttt cgttttcgcg
    17221 cattgagcga atcaacaaaa gccatcgcta aacccacggc taatccggcg atgtgtgctc
    17281 cgttcgccat cgacatccca aacaaatcaa accatccggc gacaatccag atcagcgcaa
    17341 agataattaa cccacgttgc aggtaaatgc cactttgcgg atcgcgttcg ccacgtagcc
    17401 agacgtagcc catcagcgca tacaccacgc cagaaagccc gccaaaccac ggcccgctga
    17461 atttttgctg catatagccg cttaacaggg cgctgataag cgtaatgaca attagcttac
    17521 cgctaccgag gcgtttttcc accgcgccgc cgagatacca ccaccaaagc aggttaaaga
    17581 ggatatgcat cagcgagaag tgcattaacg cgtgggtgaa gtaacgccag aactcaaatt
    17641 tcagtgttgg atcgaatggc caggccagcc ataacatcac ttcctgatcg ccgagaattt
    17701 gcatggcaat aaacaccacc acgcaggcga tcatcatcac ccaggtcacc ggacctgcgc
    17761 gttcacgcaa ggcagcaaag aaaggataac ggcgataatg caggccactg ccggtatggc
    17821 ctgccagcca gctggccgcc agataacgcg gatctgccgg gttttcgaga aaacgcgcca
    17881 gctccgcccg cacgcgctcg gcctgggact catccgccag ccagacatcg ctttggttat
    17941 gttgttgaat cgtgaggata acaccctgcg tcgccatgta atcaacaaac gcctgcgcca
    18001 cgcgggggtt agcaaaagag gtaatcatca acatcgttgc tgtcgcttat tccacacaaa
    18061 aggggacagt ataaagcgtt acgcgccgta cgccacctct gcgggaaact gtcgctgcca
    18121 ggcttcaaag ccgccgtcaa tgctatagac cacatcgtag ccctgttgca gcagatactg
    18181 cgccgcccct ttgctgctat tgccgtgata acacatcacc atcaccggag tgtcaaagtc
    18241 gttatcacgc ataaacgcgc ccagcgtgtc gttggttaaa tggaaagcct gcaccgcatg
    18301 ccccatagcg aaactctgtg gatcgcgaat atcgaccagc accgcctctt tttcctgcaa
    18361 cttctggtgc gcgtcggcaa cgttaataca ttcgaactga tccatgcgtc tctctttctt
    18421 tacaaacaag tgggcaaatt taccgcacag tttacgtcga agcggcagat aaacgccata
    18481 atgttataca tatcactcta aaatgttttt tcaatgttac ccaaagcgcg attctttgct
    18541 aatatgttcg ataacgaaca tttatgagct ttaacgaaag tgaatgaggg cagcatggaa
    18601 accaaagatc tgattgtgat aggtggcggc atcaatggtg ctggtatcgc ggcagacgcc
    18661 gctggacgcg gtttatccgt gctgatgctg gaggcgcagg atctcgcttg cgcgacctct
    18721 tccgccagtt caaaactcat tcacggtggc ctgcgctacc ttgagcacta tgaattccgc
    18781 ctggtcagcg aggcgctggc tgaacgtgaa gtgctgctga aaatggctcc gcatatcgcc
    18841 ttcccgatgc gttttcgcct gccacatcgt ccgcatctgc gcccggcgtg gatgattcgc
    18901 attggtctgt ttatgtacga tcatctgggt aaacgcacca gcctgccggg atcaactggt
    18961 ttgcgttttg gcgcaaattc agtgttaaaa ccggaaatta agcgcggatt cgaatattct
    19021 gactgttggg tagacgacgc ccgtctggta ctcgccaacg cccagatggt ggtgcgtaaa
    19081 ggcggcgaag tgcttactcg gactcgcgcc acctctgctc gccgcgaaaa cggcctgtgg
    19141 attgtggaag cggaagatat cgataccggc aaaaaatata gctggcaagc gcgcggcctg
    19201 gttaacgcca ccggcccgtg ggtgaaacag ttcttcgacg aggggatgca tctgccttcg
    19261 ccttatggca ttcgcctgat caaaggcagc catattgtgg tgccgcgcgt gcatacccaa
    19321 aagcaggcct acattctgca aaacgaagat aaacgcattg tgttcgtgat cccgtggatg
    19381 gacgagtttt ccatcatcgg caccaccgat gtggaataca aaggtgatcc gaaagcagtg
    19441 aagattgaag agagtgaaat caattacctg ctgaaagtgt ataacgcgca ctttaaaaag
    19501 cagctaagcc gtgacgatat cgtctggacc tactccggtg tgcgtccgct gtgtgatgat
    19561 gagtccgact cgccgcaagc cattacccgt gattacaccc ttgatatcca tgatgaaaac
    19621 ggcaaagcac cgctgctgtc ggtattcggc ggtaagctga ccacctaccg aaaactggca
    19681 gaacatgcgc tggaaaaact gacgccgtat tatcagggta ttggcccggc gtggacgaaa
    19741 gagagcgtgc tgccgggtgg tgccattgaa ggcgaccgcg acgattacgc cgctcgcctg
    19801 cgccgccgtt acccgttcct gactgaatcg ctggcacgtc actacacgcg cacttacggc
    19861 agcaacagcg agctgctgct cggcaatgcg ggaacggtaa gcgatctcgg ggaagatttc
    19921 ggtcatgagt tctacgaagc ggaactgaaa tacctggtgg atcacgaatg ggtccgccgc
    19981 accgacgatg ccctgtggcg tcgcacaaaa caaggcatgt ggctgaatgc ggatcaacaa
    20041 tctcgtgtga gtcagtggct ggtggaatat acgcagcaga agttgtcgct ggcgtcgtaa
    20101 attaacgtaa ggtgatcagg tcagatttca atctggcctg agactgatga caaacacaaa
    20161 actgcctgat gcgctacgct tatcaggcct acgtggttta tgcaatatat tgaatttgca
    20221 tggtcttgta ggccagataa gacgttcacg tcgcatccgg catgaacaaa gcgcactttg
    20281 tcaaaaatct aacctacttt ttaattcagg aaattaccgc aaagcccacg ccatcatacc
    20341 caacgtaaca aaactcaggc acgttcccca ctcgccccga gaaaaatagc attaatgcgc
    20401 ccagcgtcag cataaaaata ttgagcagaa ggtaaattgt tggcgtaaag agtaaattgc
    20461 aaacctcagg aatagcggca agcccagccc ccagcatggc aataaccgct gcaaaaaatt
    20521 gcgtcagtat tgggatgccg aaaaaagtca ttaccagcga ggtcaaaatc cattttctat
    20581 tttgcattat tctttccatt ctttctgaat ggcgaaatta tactccccga gtccccttgc
    20641 cccttctgga cacttttccg aaatgatggc gaaaaaaacg ggaccctttg g
//
LOCUS       DAJTSI010000069        20521 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000069 DAJTSI010000000
VERSION     DAJTSI010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.069"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="OO327_002232"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="OO327_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR6018763.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    687..812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="OO327_002233"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="OO327_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HCR6018764.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="OO327_002234"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="OO327_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR6018765.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="OO327_002235"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="OO327_002235"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR6018766.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="OO327_002236"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="OO327_002236"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR6018767.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4449..4814
                     /locus_tag="OO327_002237"
     CDS             4449..4814
                     /locus_tag="OO327_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR6018768.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4964..5482
                     /locus_tag="OO327_002238"
     CDS             4964..5482
                     /locus_tag="OO327_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR6018769.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5472..6697
                     /gene="dgcN"
                     /locus_tag="OO327_002239"
                     /pseudo
     CDS             5472..6697
                     /gene="dgcN"
                     /locus_tag="OO327_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6713..7195
                     /locus_tag="OO327_002240"
     CDS             6713..7195
                     /locus_tag="OO327_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR6018770.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVIATP
                     "
     gene            complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="OO327_002241"
     CDS             complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="OO327_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR6018771.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="OO327_002242"
     CDS             complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="OO327_002242"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR6018772.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="OO327_002243"
     CDS             complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="OO327_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR6018773.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="OO327_002244"
     CDS             complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="OO327_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR6018774.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="OO327_002245"
     CDS             complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="OO327_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR6018775.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10939..11730
                     /locus_tag="OO327_002246"
     CDS             10939..11730
                     /locus_tag="OO327_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR6018776.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11751..13037
                     /locus_tag="OO327_002247"
     CDS             11751..13037
                     /locus_tag="OO327_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6018777.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="OO327_002248"
     CDS             complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="OO327_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR6018778.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13808..14686
                     /gene="nadK"
                     /locus_tag="OO327_002249"
     CDS             13808..14686
                     /gene="nadK"
                     /locus_tag="OO327_002249"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR6018779.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14772..16433
                     /gene="recN"
                     /locus_tag="OO327_002250"
     CDS             14772..16433
                     /gene="recN"
                     /locus_tag="OO327_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR6018780.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16582..16923
                     /gene="bamE"
                     /locus_tag="OO327_002251"
     CDS             16582..16923
                     /gene="bamE"
                     /locus_tag="OO327_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR6018781.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16985..17275)
                     /locus_tag="OO327_002252"
     CDS             complement(16985..17275)
                     /locus_tag="OO327_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR6018782.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="OO327_002253"
     CDS             complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="OO327_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR6018783.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17873..18355
                     /gene="smpB"
                     /locus_tag="OO327_002254"
     CDS             17873..18355
                     /gene="smpB"
                     /locus_tag="OO327_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR6018784.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18570..18932
                     /gene="ssrA"
                     /locus_tag="OO327_002255"
     tmRNA           18570..18932
                     /gene="ssrA"
                     /locus_tag="OO327_002255"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19137..20378
                     /locus_tag="OO327_002256"
     CDS             19137..20378
                     /locus_tag="OO327_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6018785.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5299 a         5357 c         4816 g         5049 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggctaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg ggcgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt caccttgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 accatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatttgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgctcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acaggcaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat agataaattt acatcccctt taaaaacaat ccgctatgct ttgaaaaagg
     4441 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
     4501 agattgatcc ttatactcac gcgccaacct tgaccagcac cgactggtat gatgtcggta
     4561 tggaagatgc gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg
     4621 cggatcgcgg tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg
     4681 attttactta tgcccgggga ctttccggta aaagctttcc tgccagctgc aacaatgttg
     4741 aaaatgccag ccaactgcat gaagtatggc aaaaaagggc tgatgaaaat gccagcacaa
     4801 tacgattaaa ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt
     4861 tttatttctc acattgatga cggtcgcatg aaaaaatgat attttaatga gcatcctgaa
     4921 aaaatatcga tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt tttctcaccg
     4981 actgttctta cttctgattc tcctgctgac gggagcacca atactcgctc aagagccttc
     5041 tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc
     5101 tgcgctatca gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct
     5161 acaagagaat gccgcgacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga
     5221 ggcgatgatt tcaggctgca atggttttta ttttggcaac gagtcaccga cattccagat
     5281 ggaattaacc gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga
     5341 gtgcattatt ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt
     5401 aaacctggat gcctcatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc
     5461 acggaagaaa aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag
     5521 cattacgcaa catcagtatg accagcatat ttatcactat gatgctgatc tggttgctgc
     5581 tttccgtgac ctcggtgctg accctgaaac agtacgcgca aaaaaacctg gcactgacag
     5641 cagcaacaat gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa
     5701 ctgaaacact ggcagcgctg ggccagcaag ggcattttca actgcagaag tacgtgataa
     5761 gcagcaaaat attctggcat cctggcatta cccccgtaag gatccaggcg atactttcag
     5821 caatttcata agccactggc tcttccctgc ccccatcatt cagccgattc gtcacaatgg
     5881 tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt tcaatcagcc attttatctg
     5941 gttttcgctc gccgtactga ccggttgtat tctgctggca tcaggcatcg caattaccct
     6001 cacccgccat ttgcacaatg gcctggtgga agcactgaaa aatatcaccg atgtcgtaca
     6061 tgatgtgcgt tccaaccgca atttttcccg acgagtttcg gaagaacgta tcgctgagtt
     6121 tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt ggcagcttcg
     6181 tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta catgacccat taaccgggct
     6241 ggctaaccgc gcagcgtttc gtagcggcat caatacgttg atgaacaatt ccgatgcccg
     6301 aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca atgatacctg
     6361 gggtcatgcg acgggcgata gggtcttgat tgaaatcgca aaacggttag ctgaatttgg
     6421 cgggctgcga cataaagcat accgcctggg cggcgatgaa ttcgctatgg tgctctatga
     6481 tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa tctttaatct
     6541 cccgtttgat cttcataatg gccatcagac caccatgaca ttaagcattg gttacgcgat
     6601 gaccattgag cacgcctctg cggaaaaatt acaagagctt gccgatcaca atatgtatca
     6661 ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg atatgataaa
     6721 gcacctggta gcacccctga ttttcacctc actaatactg actggctgcc agtcccctca
     6781 gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta ctgaatccgc
     6841 cggcgactgg tcgctgggct tatcagatgc cattctgttc gcaaaaaatg actacaaatt
     6901 gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct cgacagggct
     6961 aacacatgcc cgtatggatg gacacaccga taactatggt gaagacagtt acaacgaagg
     7021 cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctattggtg gacaaattcc
     7081 acgcagcaat ctcaccacac agggtttagg aaaaaaatat cccatagcca gtaacaagac
     7141 cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attgcaaccc cttaaaacac
     7201 gagcaaacag acaaaaaaag ggccagccaa ttggccagcc cttcttaaca ggatgtcgct
     7261 taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt acgctcacgc
     7321 aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat gctgtcaact
     7381 accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat tttacgaaca
     7441 gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc gaatgcctgc
     7501 agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc acccggacgg
     7561 aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat gttgctcata
     7621 atttaatctc ttatcctggg taaactgatc tctcgggggc ttacgccatc ccatcatgtt
     7681 tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct tgctcttcag
     7741 tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc agcgactgtt
     7801 tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc ggaacttcca
     7861 tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct tcagcaaagg
     7921 aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg gaaacggagt
     7981 caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg attgaccatt
     8041 cttcgtcaat ttcggtttgg atcacgcgct catctatacc ttcgtagcga ccgcacacca
     8101 gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc ttgcgtccct
     8161 gtggtgacag ataaatcacc tttgcgcctt cacccgccgc ggcttttgct gcatgaatgg
     8221 cgtcccgcaa gggttgcacc atcattaaca tccccggtcc gccgccgtaa ggacgatcgt
     8281 ccacggtacg gtgccggtca tgcgtgaagt cgcgaggact ccaactctgg atgctcagca
     8341 ggccattttt aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg aacatttcag
     8401 gaaacaggct aattatgcca atccacatag cgccgccttt taccgtttat ccggtggttt
     8461 aaaaaccagg atcccaatct acttcgattg aacgagtagt gagatcgact ttcttgataa
     8521 cctgcccatc gaggaacggt acgagacgtt ccttgatacc aaacgcatct ttcaggtttg
     8581 ccttaatgac gagaacgtca ttagatccgg tttccatcat atcaacgact ttaccgagat
     8641 cgtagccttc agtggttact acctggcagc ccatcaggtc tttccagtag tagtccccct
     8701 cttcaagctg aggcagctgc gatgaatcca cgacaatttc acaattcgtc agcaggttcg
     8761 ccgcatcacg atcgtcaacg cctttcagct tgatgatcat gtcctgattg tggtgcttcc
     8821 agctttccag ctggacttgc tgccactgac ccgccttctg gataaaccag ggctgatagt
     8881 caaaaatgct ttcggcgtct tcggtggaag aaaacactct gagccaccca cgaataccgt
     8941 aagacgaacc catttttccc aaaacgatgg gatcaacagg tgcttgcgcg gtgagttgtt
     9001 tgctcatcat gaccaccgtg acagattaag ctgctttgtt tacttctttg atcagcgcag
     9061 caacgcgatc agaaatagtt gcgccctggc caacccagtg agcgatgcga tccagatcca
     9121 ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg cgctcgatga
     9181 agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac ctggtagaac ggacgctttt
     9241 tagcgccgtg acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg aataaaacaa
     9301 ccggacccca tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa ttttactgat
     9361 ttctggagaa aatgcaatca gcagttaata actctgtcgc ataaggtcat ttagcgacca
     9421 ggaaatcccg gcggcatcat tcccttcatg cttctcatca tcttcgccat tccgcctttc
     9481 ttcattttct tcatcatgcg ctgcatgtcg tcgaactgct tcagaaggcg gttaacgtcc
     9541 tgtacctgca tcccgcaacc ggcggcgata cgacgcttac gcgaaccttt gatgatttct
     9601 ggcttagcgc gctctttcat cgtcattgag ttgatgatgg cttccatacg caccagcact
     9661 ttatcgtcca tctgcgactt gacgttatcc gggatctgcc ccatgcccgg cagtttgccc
     9721 atcaagctgg ccatgccgcc catatttttc atctggcgca gttgctcaag aaagtcgttg
     9781 agatcgaagc cgtcgccttt ttttagcttg ctggctaatt tctctgcctg cgcgcggtca
     9841 actttgcttt cgatatcttc gatcagcgac agtacgtcgc ccatgccgag aatacgcgac
     9901 gcgatgcggt ccggatggaa cggctccagc gcctcagttt tctcgccaac accgaggaac
     9961 ttgatcggtt tgccagtgat gtgacgaata gagagcgccg caccgccgcg ggcatcgccg
    10021 tccactttgg tcaacactac gccggtaagc ggtaacgctt cattgaatgc ttttgccgta
    10081 ttggccgcat cctgaccggt catggcgtca accacaaaca gggtttcaac cgggttaatc
    10141 gacgcatgga cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag acgaccagcg
    10201 gtatccacca gcagcacgtc gtagaatttc agtttggctt ctttcagcgc cgcgttaaca
    10261 atatcaaccg gcttctgacc aacatcagaa gggaagaaat caacgcccac ctgctctgcc
    10321 agcgtctcaa gctgtttgat tgccgccggg cgatagacgt cagcagaaac caccagcact
    10381 ttcttcttgt gcttctcgcg caggaaatta ccgagtttac caacgctggt tgttttaccg
    10441 gcaccttgca ggcctgccat cagcacgacc gcaggcggtt gcgcagccag attcagggtc
    10501 tggttctctt cgcccatcgc cgcaaccagt tcgttacgga cgattttgac gaactcctgc
    10561 cccggcgtca ggctcttatt aacttcatga ccaaccgctt tctcttttac gcgattgata
    10621 aactcacgca ctaccggcag agcgacgtcc gcctccagca gcgccatgcg cacttcgcgc
    10681 agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg
    10741 cgcgacaaac gatcggttaa attatcaaac attgtctctc gcctggggtg gaaacggttg
    10801 gccgcaatcg cgacacatca tcagtatttt gccgcagtat aacatgaagg cgtctttgtt
    10861 gttatgcaac ggttggagca gcgttcacct gacgctatac tgcttctctt tcttattgct
    10921 caaactgtcg acattactat gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc
    10981 agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg gcggctggcg gcgcatggct
    11041 attatttctg cggtcattgc gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg
    11101 cccgacggtg atagcggaca aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg
    11161 gtgatctgta cggtaatgac cattgtggct tctcgcaatc gtggctggtt gctgctaccc
    11221 attgtctatg cctttgcgct tatcaacctg gcgctggcga ccttcatgcc caatgaatac
    11281 atcacccatc tggaagcaac gcctgggatg ctgatgcaca ttggcttatc gctcttttcc
    11341 tatgccacgc taattatcgc cgccctgtac gcgctgcaac tggcgtggat tgattaccaa
    11401 ctgaagaaca agaagctggc atttaaccaa gaaatgccgc cattgatgag tatcgagcgt
    11461 aaaatgttcc acatcacgca gattggcgtg gtgctgctaa cgctcacgct ttgcactggc
    11521 ctgttctaca tgcacaacct atttagcatg gaaaatatcg acaaggctgt gctctctatc
    11581 gtggcgtggt ttgtctatat tgtgctgctg tggggacatt accatgaagg atggcgtgga
    11641 cgccgcgtcg tctggtttaa cgttgcgggc gcggtaattc tgacactggc ctacttcggc
    11701 agccgaattg tccagcagtt aatcagctaa acccagaaaa ggagtttccc ctggaacaca
    11761 tttctactac tacgttgatc attattctga tcatcatggt ggtcatttca gcctattttt
    11821 ccgggtccga aaccggaatg atgaccctca accgctatcg tcttcgacat atggcgaaac
    11881 agggtaatcg ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac cgcctgataa
    11941 gcctggtgtt aatcggcaat aacctggtca atattcttgc ctccgcgctc ggcactattg
    12001 tcgggatgcg tttgtacggc gatgcgggcg tggcaattgc gactggtgtg ctgacttttg
    12061 tcgtactggt ttttgctgag gtattgccga aaaccattgc cgcgctgtac ccggaaaaag
    12121 tcgcttatcc gagtagtttt ctgctggctc cgctgcaaat tttgatgatg ccgctggtct
    12181 ggttgctgaa tgctatcacc cgtatgctga tgcgcatgat gggtatcaaa accgatatcg
    12241 tggttagcgg ctctttgagc aaagaagagt tgcgcactat cgtgcatgaa tcgcgctcac
    12301 aaatttcccg tcgcaatcag gatatgctgc tgtcggtgct cgatctggaa aaaatgaccg
    12361 ttgatgacat catggtaccg cgcagtgaaa ttatcggtat tgatatcaac gatgactgga
    12421 aatctattct gcgccaactc tcccactcac ctcacgggcg catcgtgctt taccgtgatt
    12481 cgctggacga cgccatcagt atgctgcgag tacgtgaagc ctggcggttg atgtcggaga
    12541 aaaaagagtt caccaaagaa accatgctgc gcgccgcgga cgagatctat tttgtgcctg
    12601 aaggtacgcc gctcagcacg cagttggtaa agtttcagcg caacaaaaag aaagtcggcc
    12661 tggtcgtcaa cgagtatgga gacattcagg ggctggtgac ggttgaagat attctggaag
    12721 agattgttgg cgatttcact acgtcgatgt cgccaacact tgccgaagag gtcacgccgc
    12781 aaaacgacgg ttcggtgatt atcgatggca ccgccaacgt gcgggaaatc aacaaagcct
    12841 ttaactggca tctaccggaa gatgatgccc gcacggttaa tggcgtcatt cttgaggcac
    12901 tggaagagat ccctgtcgca ggcacccgcg tgcgtattgg caagtacgat atcgatattc
    12961 tcgacgtaca ggacaatatg attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg
    13021 agagtgtggc ggagtaacga aaacggccct gcattcgcat gcagggccgt gaattattac
    13081 gaaaacagaa attacgcttt tgctttcgct acagttacca tcgccgcacg aatcgtacga
    13141 ccattcagcg tataaccctt ctgcataatg cccagtacgt tacctggcgc aacgtcatca
    13201 gattccacca ttgcgatagc ctgatgcaca ttcgggtcca gcggcacgtt agtttcggcg
    13261 atcacttcaa caccaaactt acgcacaaca tccagcatcg acttcagcgt cagctcaatg
    13321 ccttcaacca tcgcagacat atccgggtta gctttatcag ccacttccag cgcacgatcc
    13381 aggctatcaa tcaccggcag caattcgttg atgaatttct ccagcgcgaa tttgtgggct
    13441 ttttcaatat ccagttcagt acgacgacgc aggttttcca tttcggcttt tacgcgcaaa
    13501 ataccgtcac gttcacgggt ctgggcttca gccagctgag cttcgagatt cgcaattttt
    13561 tcatcgcgcg gatccacctg ctcagcagaa gcttctggct caactgcctc aatctcttcg
    13621 tgctgatcca tgataatttc ttccggggct tgcccctcag gcgttttctg ttctttacta
    13681 ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct aacttcgctt attatgggga
    13741 tcagtttcag ggtttcaagg gaagcactca cattgtcatc aatcttcgca acaaggacct
    13801 cggaaaaatg aataatcatt tcaagtgtat tggcattgtg ggacacccac ggcaccctac
    13861 tgcactgaca acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt acgaggtcat
    13921 cgttgagcaa caaatcgctc acgaactgca actgaagaat gtgaaaactg gcacgctcgc
    13981 ggagattggg caactggctg atctcgcggt agtcgttggt ggcgacggta atatgctggg
    14041 cgcggcgcgc acgctcgccc gttacgatat taaagttatt ggaatcaacc gtggcaacct
    14101 gggtttcctg actgaccttg accccgataa cgcccagcaa caattagccg atgtgctgga
    14161 aggtcactac atcagcgaga aacgtttttt gctggaagcg caagtctgtc agcaagattg
    14221 ccaaaaacgc atcagcaccg cgattaacga agtggtactt catcctggca aagtggcgca
    14281 tatgatagag ttcgaagtgt atatcgacga gatctttgcg ttttctcagc gatctgatgg
    14341 gctaattatt tcgacgccaa caggctccac cgcctattcc ctctctgcgg gcggtcctat
    14401 tctgaccccc tctctggatg cgattaccct ggtgcccatg ttcccgcata cgttgtcagc
    14461 acgaccactg gtcataaaca gcagcagcac gatccgtctg cgtttttcgc atcgccgtaa
    14521 cgacctggaa atcagttgcg acagccagat agcactgccg attcaggaag gtgaagatgt
    14581 cctgattcgt cgctgtgatt accatctgaa tctgattcat ccgaaagatt acagttattt
    14641 caatacatta agcaccaagc tcggctggtc aaaaaaatta ttctaatttt acgccagcct
    14701 ctttactgta tataaaacca gtttatactg tacacaataa cagtaatggt ttttcataca
    14761 ggaaaacgac tatgttggca caactgacca tcagcaactt tgctatcgtt cgtgagcttg
    14821 agattgattt tcatagcggc atgaccgtaa taactggcga gaccggtgcg ggtaaatcta
    14881 ttgcaataga tgccctcggt ctttgtctcg gtggtcgcgc tgaagccgac atggtgcgta
    14941 ccggcgctgc tcgcgctgac ctgtgcgccc gtttttctct gaaagatacg ccagcggccc
    15001 tgcgctggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt cgtcgcgtga
    15061 tcagcagtga tggtcgctcc cgtggtttca tcaacggtac agctgttcct ctgtcacaac
    15121 tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat caattactca
    15181 ccaaacctga gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc tctctacttc
    15241 aggaaatgac cgcacgttat cagttgtggc atcaaagctg ccgtgacctc gcgcatcatc
    15301 aacagttaag tcaggaacgc gccgcccgtg cggaactgct gcaataccaa ttaaaagaac
    15361 ttaacgaatt taatccgcag cccggagagt ttgagcaaat cgacgaagag tacaaacgtc
    15421 tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta atggccgacg
    15481 gtgaagacgc aaacctgcaa agtcagcttt acacggctaa acaactggtg agcgaattga
    15541 ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaagct accatccaga
    15601 ttgctgaagc cagcgacgaa ctgcgccact actgcgatcg tctggatctc gatcccaacc
    15661 gactgtttga acttgaacag cgcatctcaa aacagatttc gctggcacgt aaacatcacg
    15721 tcagccctga ggcattacca cagtattacc agtcgctact ggaagaacag cagcaactgg
    15781 acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat catcagcagg
    15841 cactggaaac cgcgcgcgca ttacaccaac aacgccagca atatgcagaa gaacttgcac
    15901 agctgatcac cgacagtatg catgcgctct caatgccgca tgggcagttt acgattgatg
    15961 tcaaatatga cgagcatcac ctgggcgctg acggtgccga tcgtattgag tttcgggtaa
    16021 ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt ggtgaattgt
    16081 cccgcatcgc actggcaatc caggtcatca cggcgcgtaa aatggaaacc ccggcactga
    16141 tttttgatga agtggatgta gggattagcg gtccaacagc ggcagttgtc ggcaaactgc
    16201 tgcgtcaact cggcgaatca actcaggtga tgtgtgttac ccacctgcca caagtcgcgg
    16261 gatgtggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg acagaaacac
    16321 atatgcaatc tctggataaa aaagcgcggt tacaagagct ggcgcgcctg cttggtggca
    16381 gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcagcg taaacttttt
    16441 tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc aaaaggtttt
    16501 aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta ctgctcgggc
    16561 ccgaaaagga atcaaatcac tatgcgctgt aaaacgctga ctgctgcagc agcagtacta
    16621 ttgatgttaa ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc tgacatcaac
    16681 caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat gacgcaacaa
    16741 caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac gaatacctgg
    16801 ttctatgtct tccgccagca accaggtcat gaaggtgtaa cgcagcaaac gctgacgctg
    16861 acctttaaca gtagcggtgt gttgaccaat attgataaca aacctgcgct gagtggtaac
    16921 taataataaa gttgctctca aagacgttaa aaaaggtgct caatgagcac cttttttctg
    16981 tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc ggatcggcaa
    17041 tgagagggcg ataaatctcc acccgatcgc catcatgcac actatcgctt agttttgccg
    17101 gacggctgta aatgccgact ttgtttttag ttaaatcgat atcggtacgc aattccagca
    17161 agccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc actcgctgca
    17221 ggtattgctt ctcaggtagc gcataagcca cctcaacggc aattttacct agcactgtaa
    17281 acctctttcg cacgaaccgt aaaagcctgg accatattag ccgccagctc tttaaacacg
    17341 cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg aaactcgata
    17401 cgacacgcct cctggctcag cggcgtaaac ttccatccac caatcaattt cttgaacggc
    17461 ccatccacca gattcataag aatactttgg ttactggtca actggttgcg ggtagtaaac
    17521 gttttgctga tcccagcctt agagacatct accgccgcag tcatctgccc aggagtggac
    17581 tccagaatcc gacttccggt acaacctggc aaaaactgag gataagactg aacgtcattc
    17641 actaactgat acatttgctc cgcgctgtag ggtaccagtg cggtccggct aatctgaggc
    17701 ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc tgtaaaaaaa
    17761 acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt gtcccccgca
    17821 ggattgatat ggggtatttt cgatttcaga ttaccgatga ttcacgacgc ttatgacgaa
    17881 gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg cccgtcacga
    17941 atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg aagttaaatc
    18001 cctgcgcgca ggaaaagcca atatcagcga cagctacgtc cttctgcgtg acggagaggc
    18061 atttctgttt ggcgctaaca tcacgccaat ggccgtggcc tccacgcatg tggtgtgcga
    18121 tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat tgtacggtcg
    18181 cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa atgcctggtg
    18241 caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt cagatatcaa
    18301 agaacgcgaa tggcaggtgg ataaagcacg catcatgaaa aacgcccacc gttaaacctg
    18361 cactccaatt attgaccagt tcctcaccgc gcctccctct ccggcggcgc gaatgaacat
    18421 cttattggct atcacatccg acacaaatgt tgccatccca ttgcttaatc gaataaaaat
    18481 caggctacat gggtgctaaa tctttaacga taacgccatt gaggctggtc atggcgctca
    18541 taaatctggt atacttacct ttacacattg gggctgattc tggattcgac gggatttgcg
    18601 aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa aaatagtcgc
    18661 aaacgacgaa aactacgctt tagcagctta ataacctgct tagagccctc tctccctagc
    18721 ctccgctctt aggacgggga tcaagagagg tcaaacccaa aagagatcgc gtggaagccc
    18781 tgcctggggt tgaagcgtta aaacttaatc aggctagttt gttagtggcg tgtccgtccg
    18841 cagctggcaa gcgaatgtaa agactgacta agcatgtagt accgaggatg taggaatttc
    18901 ggacgcgggt tcaactcccg ccagctccac caaaattctc catcggtgat taccagagtc
    18961 atccgatgaa gtcctaagag cccgcacggc gcaagccctg cgggcttttt tgtgctccga
    19021 atttgtcccg cgaagtctga tgccaactaa ttaaatccga tccttttagg caccttgtta
    19081 ggcacttcat aaagctatat tgtctttgag gtgcctaaaa ctatggaaac ccggcaatgg
    19141 caagacaaac caaacctcta tccgttaagg aaatcgaatc tgctaaaccc aaggaagcgg
    19201 actacgttct ctatgatggc gatggccttg agctactcat taaatccagc gggagtaaaa
    19261 tctggcagtt tcgctacatt cgccctgtca ccaagacacg agcaaagaaa agcataggcc
    19321 cttacccgtc agttaccctt gccgatgcca gaaactaccg tgcagagtcc cgctcactcc
    19381 tagctaaaca gattgatccg caggaacatc agcaagaaca attgcgtagt tcgctggaag
    19441 ccaaaaccaa tactttccag ctcgtagctg aacgttggtg gaatgtgaag aaagccagtg
    19501 tgacagagga ctatgcggaa gatatctggc gctctcttga aagagatgtc tttcctgcga
    19561 ttggcgacgt tagcgttaca gatattaaag ctcatacact ggttcaggcc gtacaaccgg
    19621 ttcaggccag aggagcactg gaaacggttc gtcgcctgtg tcaacgtatt aatgaggtca
    19681 tgatctatgc ccagaacaca gggctgattg atgctgttcc cagcgttaat atcggtaaag
    19741 ccttcgaaaa gcctcagaaa aagaacatgc ccagcattcg accggatcag ctatctcaac
    19801 tgatgcagac aatgcgaaca gccagcatta gcctttccac acgctgcctg ttcatgtggc
    19861 aacttcttac tattacccgc cctgccgaag cggctgaagc tcgctgggaa gaagtagaca
    19921 tagaagcgca agagtggaag attcccgcag cacgcatgaa aatgaaccgc gaccatactg
    19981 ttccattgtc agatgaagca atagctgtgc tggagatgat gaaaccgcta agcggcaacc
    20041 gagaatttat ctttcctagc cgtatcaagc ccaaccagcc tatgaacagt cagactgtta
    20101 acgcctcact aaaacgcgca ggttttggtg gtgtgcttgt ttcgcatggt ctgagatcaa
    20161 ttgccagtac ggctcttaat gaacaaggct ttccgcctga tgccattgag gcagcattgg
    20221 cccatgtgga taagaatgag gttcgtcgtg cttataaccg cagcgattac ctggaacagc
    20281 gtcgcccgat gatgcaatgg tgggcaaatt ttgtgatggc agcggatcgt ggaagcatga
    20341 ttgaaggtgg gataaaagga atgaagctag ttgggtgact aaatagctgc gcggcatagt
    20401 gtcccacttt cagccagccc ttgcaggtta agccaccaga cagtaaacgg cgggtgtcgc
    20461 gttattaggc gcagctattt agtagtaata ttaggattgg tgattctgca ctccagaagt
    20521 t
//
LOCUS       DAJTSI010000070        20304 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000070 DAJTSI010000000
VERSION     DAJTSI010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.070"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..444)
                     /locus_tag="OO327_002257"
     CDS             complement(<1..444)
                     /locus_tag="OO327_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCR6018786.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLL"
     gene            complement(499..1350)
                     /gene="manZ"
                     /locus_tag="OO327_002258"
     CDS             complement(499..1350)
                     /gene="manZ"
                     /locus_tag="OO327_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR6018787.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1363..2163)
                     /gene="manY"
                     /locus_tag="OO327_002259"
     CDS             complement(1363..2163)
                     /gene="manY"
                     /locus_tag="OO327_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR6018788.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVLGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2226..3197)
                     /gene="manX"
                     /locus_tag="OO327_002260"
     CDS             complement(2226..3197)
                     /gene="manX"
                     /locus_tag="OO327_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR6018789.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3660..5210
                     /gene="yoaE"
                     /locus_tag="OO327_002261"
     CDS             3660..5210
                     /gene="yoaE"
                     /locus_tag="OO327_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR6018790.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5214..6812)
                     /locus_tag="OO327_002262"
     CDS             complement(5214..6812)
                     /locus_tag="OO327_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6018791.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6943..8307)
                     /gene="sdaA"
                     /locus_tag="OO327_002263"
     CDS             complement(6943..8307)
                     /gene="sdaA"
                     /locus_tag="OO327_002263"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6018792.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8491..9069)
                     /locus_tag="OO327_002264"
     CDS             complement(8491..9069)
                     /locus_tag="OO327_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR6018793.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVTP"
     gene            complement(9073..10434)
                     /gene="pabB"
                     /locus_tag="OO327_002265"
     CDS             complement(9073..10434)
                     /gene="pabB"
                     /locus_tag="OO327_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR6018794.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10508..10687
                     /locus_tag="OO327_002266"
     CDS             10508..10687
                     /locus_tag="OO327_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR6018795.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10807..11166)
                     /locus_tag="OO327_002267"
     CDS             complement(10807..11166)
                     /locus_tag="OO327_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR6018796.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11528..11872)
                     /locus_tag="OO327_002268"
     CDS             complement(11528..11872)
                     /locus_tag="OO327_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6018797.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            12004..13914
                     /locus_tag="OO327_002269"
     CDS             12004..13914
                     /locus_tag="OO327_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR6018798.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13972..14667
                     /gene="tsaB"
                     /locus_tag="OO327_002270"
     CDS             13972..14667
                     /gene="tsaB"
                     /locus_tag="OO327_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR6018799.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14707..15288
                     /gene="yeaY"
                     /locus_tag="OO327_002271"
     CDS             14707..15288
                     /gene="yeaY"
                     /locus_tag="OO327_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR6018800.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15494..17179
                     /gene="fadD"
                     /locus_tag="OO327_002272"
     CDS             15494..17179
                     /gene="fadD"
                     /locus_tag="OO327_002272"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR6018801.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17261..18376
                     /gene="rnd"
                     /locus_tag="OO327_002273"
     CDS             17261..18376
                     /gene="rnd"
                     /locus_tag="OO327_002273"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR6018802.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18430..19395)
                     /gene="yeaX"
                     /locus_tag="OO327_002274"
     CDS             complement(18430..19395)
                     /gene="yeaX"
                     /locus_tag="OO327_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR6018803.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIVGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19451..20068)
                     /gene="yeaW"
                     /locus_tag="OO327_002275"
                     /pseudo
     CDS             complement(19451..20068)
                     /gene="yeaW"
                     /locus_tag="OO327_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
BASE COUNT         5006 a         5077 c         5267 g         4954 t
ORIGIN      
        1 gagaagctta taaatttttt ccagatcgtc gatatttcga acgcggatta acagccgacg
       61 ctgttctaat tgcatcacca acacgccatc ttccgacaag ttcatcgctt tgattcggct
      121 atattcaatc cagacattgg cgaagaaaaa acctttttgt ttaaagatga tcttcggaat
      181 gcggatccag aatatataaa aacccatcag agccagtgca cttaataacc atgttgttat
      241 tagcgcgcca tgattcgtga cgttgttata gataagaatg acaatcaatc cgacgaagat
      301 aacgctatcg atgcgaccac gccggagcaa aggaattgcc agcagggtgg ggccgttacg
      361 gcggggcatg atgaactgat cgtagatcgc gaaggccagg agtgcggcga tgaaaagaat
      421 cagtaccagg tccgtgattg tcattaatcc tccagataaa aaaacggggc caaaaggccc
      481 cggtagtgta cgacagtctt acagtcccag caggccgcaa gcgtaaccag cgataccgat
      541 gacgaagaag ccaacgatga tccacagcgg gttaactttt ttgcgcagca gccacataca
      601 agcaaatgtc agcagcagtg gtacaaggcc tggcattaac tggtcaagaa tagtttgcac
      661 ggtggtgaca cgggtctgtc catcctgccc ggtgatggtc gaaaccacca gcgggatgtt
      721 gacatgtgtc cacttgttaa ccaatgcccc catgacaaac aggccgagga tagacgcccc
      781 ttccgtcagt ttttgcagga agccaccacc catatcttta acgatatcga tacctttgga
      841 gtaaccatac gctacgccgt agtaacgggt tgccagacgc accaggttaa acaggatgaa
      901 gaacagcagc ggacctaaca ggctgccgct catcgcgata ccggcaccca gtgctgcaaa
      961 taccggacgt acggttcccc agaagatcgg gtcgcctaca ccagccagcg gccccatcaa
     1021 accgactttg ataccgttga tagcaccgtc atcgatctct gcgccattag cacgctgttc
     1081 ttccagcgcc agggttacgc cgagaatcgg tgcagccacg aacggctggg tgttaaagaa
     1141 ctccaggtga cggcgaatag cttgtttacg agcttcgttg ttctcagggt agaggcgacg
     1201 aattgccggt accatagaga agcagaaacc cagtgcctgc atacgttcga agttccatga
     1261 accctggaag aggttagaac gcaggaatac gccacgaata tcactttgag tgagtttttt
     1321 ctcggtggta gtttgagttg tatcaaccat ttcgctcacc tgttagtcca gttcgttatc
     1381 gagatcgttg ttaccagctg cctgagcagg cgcaccggct acgcggttgt atttcgggct
     1441 aagttggatg tagagcactg ccataacagt accaagcaca cccagagcaa ccaggttaaa
     1501 gttggtgaat gctgcggtta cgaagccgag gtagaagaac ggcatcaggt agccagcacg
     1561 catcatgttg ataaccatcg cataaccaac caccacgatc atgcccccgg cgatattcag
     1621 accattggtc accacttccg gaatcgcatt cagcatgttc tgtacttcgc tggtaccaac
     1681 agacagcgca acgatgacgg ccggaatagc cacacgcatt gcttgcagga acagagaaga
     1741 aacgtggatc caggaaatcg ctgtcaggtt gccgttatca gcagccttat ccgcagcgtg
     1801 ctggaaagca acggtaatag tacgaacgat gatggtcagt acctggcccg cagcggccag
     1861 agggattgcc agtgcgatac ctgcaccaat gctctgatga cctgcgataa ccagaatggt
     1921 agaaatgata gaagccagag cggcgtcagg cgcaactgca gcaccgatgt tcatccagcc
     1981 cagcgcgatc atttccagcg taccaccgat aataataccg gttttcatat ccccaagaac
     2041 gatacccacc agggtacacg cgattagcgg acggtgaaac tgaaattcat cgaggattga
     2101 tcccatacct gcgatacagg ctacgataaa taccagcaca atttgaagag tggtaatctc
     2161 cattgtactt ctcctgttta cgtaagactt aagtgtgaaa actgaatgat aatcaacaca
     2221 atacgttact tatcgatttt gctgatcaga tccatcattt tcagtttcgg atcggtggaa
     2281 accttacgga cttccagctc aataccgcgt gcattcagtt ttttgaacgc ctcgatatct
     2341 ttttcatcaa ccgaaaccgc gttattcacc tgggttttac cctgacggaa tgccatacca
     2401 ccgacgttaa cagaggtgat tttcacgccg ccttcaacga gacgctctac atctgttggg
     2461 ttggtaaata acagcattac gcgttcgcca gcatatttcg ggttgttgta gacgcgaatc
     2521 attttggcaa catcaactac gtgtgctgtt acgcccggag gtgcaacctg ggtgagcagt
     2581 gtcttacgaa cggtatccgc agccacttca tcactaacaa caataatacg ggagacattg
     2641 gtttctttgg tccagcgggt ggcgacctga ccgtgaatca gacggtcgtc gatacgcgca
     2701 aggccaataa ccatgtagtc gtttggcccc attggttttg ccggagttgg agccgctttt
     2761 ggtgctgcgg cagcgggtgc tggcgcggct ttttcaaccg gtttggcttt cagtgctttc
     2821 acgccttcac ggcctgtttc tactgccagc gcaaccagtt catcaaagct cgggtcatca
     2881 tcacgggcca ttaacgtttc cacgagcatt ggaatgttaa cgcctgcaat gacttcataa
     2941 tgctctttgt cgacgacaat gcggctggca gcattgaacg ggctgcctcc ccatgtatca
     3001 acgagaaaca gcacgccttt agtggtgtcg agttttgcca actgagcgtt gtatttttca
     3061 atcagcgttt cggcattttc acctggaacg aaatcgatcc agccgacgtt ttcctgctcg
     3121 cctaacagca tttctgccgt tttaagcaac tgctctgcag cccaaccatg tgtgcctata
     3181 acaatagcaa tggtcacttg ctacctcctt tattatcgtt aacacctcaa cgtgccagat
     3241 gtatttttga atcgtcgtcc acaatcgaat cgattcagat aagggctaca accaaaaacc
     3301 cctgcgtttc gcgatttatt ttagatatcg aaaaaattat tttatgtgat gaagatccgt
     3361 aatttaactt tcgattatca gaaatttcga aaggtaaaat atccttgtca cattcgtttg
     3421 caaaggaagg taaatctttg ccaaatcaga ggcgtctctg atatgtttaa ctcccgttta
     3481 acaaccatgg agtatagggc agtagcccgc agtatggatc gtcaccgacg tcatttcagc
     3541 atcaggcctt ttaacgcctg cctttctggc actctatgcc gcacctttcg tttgcatttt
     3601 gtcgttacgc ctgcattatt tctggcgtcg aatagctatt ccttaagcag gagcttgtca
     3661 tggaattctt aatggacccc tcaatttggg cggggctact cacgcttgtt gttctcgaaa
     3721 ttgtgctggg tatcgataac ctggtcttca tcgccattct tgctgacaaa ctgccgccaa
     3781 aacaacgcga taaagcgcgt ttgctggggt tatcactggc gctgattatg cgtctggggc
     3841 tgctgtcgct gatttcatgg atggtcacgc tgaccaaacc gctatttacc gtcatggatt
     3901 tctccttctc cggacgcgat ctgattatgt tgttcggggg gatattcttg ctgttcaaag
     3961 caacaaccga actgcatgaa cggctggaaa accgcgatca tgattccggc cacggtaaag
     4021 gctacgccag tttctgggtg gtcgtcacac agattgtcat ccttgacgcc gtcttctcgt
     4081 tggatgcggt aattactgca gtagggatgg ttaaccatct gccggtgatg atggcggcgg
     4141 tagtgattgc gatggcggtt atgttgctgg catcgaaacc gctgacgcga ttcgttaacc
     4201 agcaccccac ggtggtggtg ctctgtctga gcttcctgtt aatgattggt ctgagtctgg
     4261 tggcagaagg tttcggtttc cacattccga aaggttacct gtatgccgcg attggcttct
     4321 cgatcattat cgaagtgttt aaccagattg cgcgtcgcaa ctttattcgt caccagtcga
     4381 ctttgccgct gcgagcgcgt actgccgatg ccatcctgcg tttgatgggc gggaaacgtc
     4441 aggccaatgt tcagcacgat gccgataacc cgatgccgat cccggaaggt gcatttgccg
     4501 aagaagaacg gtacatgatt aacggcgtac tgacgctggc gtcgcgttct ctgcgcggga
     4561 tcatgacgcc gcgcggtgaa ataagctggg ttgacgctaa tctcggggtc gatgaaatcc
     4621 gcgagcaact gctctcttca ccgcacagtc tgttcccggt atgtcgcggt gaactggatg
     4681 aaatcatcgg tatcgtacgt gctaaagaac tgctggtggc gctggaagag ggcgttgatg
     4741 tggcggcgat tgcttcggcg tctccggcga ttatcgtccc ggaaaccctc gatccgatca
     4801 acctgctggg cgtgctgcgt cgcgctcgcg ggagctttgt tatcgtgacc aacgagtttg
     4861 gtgtggtaca aggcttggtc acgccgctgg atgtgctgga agccattgcg ggtgaattcc
     4921 cggacgctga cgaaacgccg gaaatcatta ccgatggtaa cggctggctg gtaaaaggcg
     4981 gtacagattt gcatgccttg cagcaggcgc ttgatgttga gcaccttgcc gatgacgatg
     5041 atatcgcgac ggtcgcgggc ctcgtgatct cggcaaatgg tcacattccc cgtgtgggcg
     5101 atgtgattga tgtagggcca ctgcatatca ccatctttga agccaatgat tatcgtgttg
     5161 atctggttcg cattgttaaa gagcaaccgg cgcacgatga agatgagtaa gcgttaacgt
     5221 aacgacataa tgggcgtgat atgtccatta tgccgggcgg gcggcggttg gctgcccgcc
     5281 agccatttgg gaaaatcacg tagcggcatc ggctgtgcgt acaaataccc ttgcaaaata
     5341 tgcacgccat gacggcgcaa atacttcgcc tgttcttgcg tttccacgcc ctccgccacc
     5401 agttcaatat tcagtctttg ccccagcgcg atgatgatat cggtcaccgt cgagttaacc
     5461 gcgtcagaac ctatagctgc ggtaaatgac ttatcaattt tcagcacgtc aggacgtaat
     5521 gtttcaagcc aggaaaacga gctgttgccg gtgccgaagt catcaatcgc cagtttgacg
     5581 tttttacgat gcagctcgcg ggcaatccga taatcaacat ccagtaaggc atcgcgttcg
     5641 gtgatttcga ggatcagttg ctgaatcggg tgagcgctaa accagtactg attgagatct
     5701 tttatcagca caccacggcg aaaatggctg ggtgcgacgt taatgccaac atgaaactga
     5761 ctactcatcg ggaaaacatg gtgctgacga atggtttctg ccatcacata gtgggtcagt
     5821 ggcacaatta aatgatgttc ttccgcgata ggaataaaca catccggtga aatccagccc
     5881 tgacgcggat tattccagcg cagcaaaatc tctacaccaa tacattgctg gctgcgcgca
     5941 ttaagcaaag gctgacagaa caattcgaac tcatgttgcg ccaggcccag attaatttcg
     6001 cgggaaaagc tcatccggta agcggtcgcc agccaggcga tatagcccac cagtaaactt
     6061 agcagcaccg ccagcggtaa ttgtgttgga agatagtgcc atgccagcgc cgtagccccc
     6121 ggaccattta cgttaatggt aaaaggaaag ctttgcgaag aaacctgata gttttcattg
     6181 tcttcaggtt gcggaaggga atctaccagc ccattaccat agagcaaatg ccgtttgtcc
     6241 accgtcaggc tggcgctact gatttgcggc agttgtggct caaggagcat tgccgtcagt
     6301 aagtcgatgt taatcatctc tatgacccca gcattgctac tgcccgctgc aggcgtccat
     6361 tgaatcaaaa ccggactgcc tttaatcagg gcacggtcga tcgtcaggcg taaaagtggt
     6421 tgcggtgcag gaagttcagc cagaatgtcc acgacgggca cattgcgata accaaaaatg
     6481 ctggagcaat aaagtgtgcc gtcttgcacc aggccaatgg atcgcacagt ttggagtttt
     6541 gccgcctgtt tacgtaatgg caaatgggcg acagagcagg gcagaccaat cagcggaagc
     6601 aagactttgc tacctgcctg taggggaagc agtactttat ctaattcctc tacagcgtga
     6661 ttggcgaatt gtactacccg ttgttgattt aagttacgct gtgaaataaa tcgcacgctc
     6721 agggtcgaag cgagcgtaac gagtgcgcaa atcgtacaaa caatcattcg attacggcga
     6781 taggttttaa tgatccgttg tgctttttgc atgcgggcca ccctgtaagc gtatggcgac
     6841 aaacgcctga cgcaacagtg gaagtgtagt ggggaatatt acagcagacg agaaagcggg
     6901 tataaattcg cccatccgtt gcagatgggc gagtaagaag tattagtcac actggacttt
     6961 gattgccaga ccaccgcgtg aggtttcgcg gtatttggcg ttcatgtcct taccggtttc
     7021 gtacatcgtt tcgatgacct tatccagcga gacgcgcggt gcactggtgc ggcgcagagc
     7081 catccgtgcg gcgttaatcg ccttcacaga ggcaatggca ttacgctcaa tgcacggcac
     7141 ctgaacctgc cctgcaaccg ggtcgcaggt taaaccaagg ttgtgttcca tgccaatttc
     7201 cgccgccacg caaacctgtt ccgggctacc gcccagcagt tctgcaagac ccgcagcagc
     7261 cattgaacag gcaacaccca cttcgccctg gcaaccaact tccgcaccgg aaatagaggc
     7321 gttcatttta tacaatgcac caatcgcgcc cgctgccata aagtaacggg tatagatgtc
     7381 tgggctgacc gattcaataa agtggtcata gtaagccagc actgccggaa cgataccgca
     7441 ggcaccgttg gttggcgcag ttaccacacg accaccggcg gcgttttctt cgttaactgc
     7501 cagcgcaaac atgtttaccc agtcaatgac attcatcgga tcgttagaca gtttatcgct
     7561 ggaaactaac atccggcgca gggcagacgc acgacgtggc acgcgcagcg ggcctggcag
     7621 tacaccttcg gtgttcatcc cgcgatcgat acatgcctgc atggtttgcc agacatgcgc
     7681 gaaatactcg tcgatctctt tcttgctgtg cagcgccagt tcgttctgca tagcgagacc
     7741 agacagcgaa tagccggttt cattacagta cgcgagcagt tcggtggcag atttgaacgg
     7801 atacggcacg ctcacttcgt tggcagcatc ctgaccaaag tgttcttcat cgacgataaa
     7861 accgccgccg atggaataat aagttttgct gtagacgact tcatcgccgt tataggcgtg
     7921 gatttgcata ccgttttcat gcagcggcag gttgccgtta tgaaaacgca tcccgttgtc
     7981 gcgcgggaaa tccacttcat gccgtccctg tgccagcagc agacgttcgc gctcttttac
     8041 gtcgcgaata aaaccgggaa tactgtcgat atccacggtg gcaggttcgt tacctgcaag
     8101 acccataata atggcgatat cggtgtggtg gcctttaccc gtcagcgaca gtgaaccata
     8161 aacgtccacg gcaacgcgag taacgctatc cagtaagcct ttttcgacca gatcatcgac
     8221 gaactgttta cccgccttca taggccctac ggtatgggaa gatgagggac caatccccac
     8281 cttaaacatg tcgaatagac taatcacgat aatactcctg acaaggtgac tggacttcca
     8341 gtaacgaact aataacagcg catagtgtaa gagggaacgc ggcgactggc ttaactattc
     8401 acatgaatta aactaatcaa aaaccgcgat ttaactaata ttgcgatgat tgtctcaagt
     8461 agtgttttgt agatgtaaag ataagtatag tcagggggtc acaccaattt gcagcgccag
     8521 ctcacgaatt atgcctgcgg tcattcccca tacaaaatac tgttcgtacc aggacagcca
     8581 tacccgatgt gaatcgccac ggcggtagat atctaaaggg tgataacgac ccagatgtaa
     8641 tgcctgggcg agcggcattt caaacaccgc cgagacttca tcttcactgg cgcgatacgg
     8701 cagatcgggc gggataatgc cgaccactgg ggttacttga tagccagtta cgctatcgac
     8761 gggcggcagc acgccgataa cttcaacggc ggaaggcggt atagcgacct cttcttcagc
     8821 ttcgcgcagc gcggcggcga taactgatgc gtccgtgtca tcgactgcac ctccagggaa
     8881 tgccacttgt ccagcgtgtt tacgcagatg aattgaacgc tgagtcagca acaaccccgg
     8941 ttgcggtcga cggacgatgg ggattaacac ggcagcctga cgatgattta ggggttcccg
     9001 gttaatttgc gggcgcaaaa gttgaaagcg agataaaaaa tcatcaagcg tcaggctacg
     9061 gtattccacg tcttacttct ccagttggcg taatatctta ttaactttat caaaagtttc
     9121 ctgatattcc gcttcttcct ggctatcggc gacaattcca ccgcccgcag agcagtatat
     9181 ttgtccgtta atggcagtca gcgtgcggat agtaatgctg gtatccatgt tgccgcaaaa
     9241 gctcaaatag ccaatgctgc cgcaccaggc attacgtcgc tgcggttcca gttcgtcgat
     9301 aatttccata gcccgtactt tcggagcccc ggttattgag ccaccaggaa aagctgcgcg
     9361 cagcagatcg ctggcgtgta actgttctgg tagtcgcgcc gttatagtgc tgaccagatg
     9421 atgcacggca gggaagggtt ccaccacgaa gagctctggt acttttaccg aaccggctac
     9481 ggcaacacga ccgatatcat tacgcattaa atcgacaatc atcagatttt cggcacgatc
     9541 tttcgctgag ttcgccagtt tttctgcttg tttgctatct tcctgaggat cgggcaggcg
     9601 tggtagcgtg cctttaatcg ggcgagtctg gatttcacta ttatcacaaa gaataaaccg
     9661 ctctggcgaa aggcttaaaa ttgcaccctg ttcaagacgt aaaaaagcgc taaatggcgc
     9721 gcggttggcc tgattaagct gaaggaatgc ctgccattca tcgccagaat aggtcgcatg
     9781 aaaacgctgg gcgagattca cctgatagca atcaccgctg tgcagatatt cctgtacctg
     9841 gcgaaatttt tcgccgtact gctcgtgggt catattggat tgccagtcgg aagtgagcgt
     9901 gaaatcttcc tgcggcgaga attgctggct ttccagccag gcccgacggg cattgacatc
     9961 attatgactc agcaaagaaa ctgtatgacg ctggtggtcg acaatgagcg cccaatcgta
    10021 gatacccact gccatatccg gcagaacgat atcttgttcc gcaatttctg gcagtgactc
    10081 aaaacggcgg cccagatcgt agccaaacaa ccccagtgcg ccgccctgaa atggcaaatc
    10141 ttcgttatgc gttgggcgaa tgtctgcgcg atccagcacc tgctggagca cctgtagcgg
    10201 gtcatcagtg gtcgttgtgc gtttttcgct ttcactaaca acggtttctt tacctaaagt
    10261 ggttaaagtg caaatcggat cggcgaccac aatatcaaag cggctatacg gatgatcggc
    10321 atagccggag tgtaaaagca tcgcccacgg caggtggctt aagcgggaga aataaaattc
    10381 agcggcgtcc tgacgccagg gtaaagtaat cacagtggga gataacgtct tcatcagtcc
    10441 tgactctact ggctatgtgc cgggtagcat gaaataatta gcgccaacaa tttagcagga
    10501 gttaacaatg tttgcaggtt taccttcact catccatgaa cagcagcaaa aagctgtcga
    10561 gcggatccag gaactgatgg cacaagggat gagtagcggc caggcaatcg cgctggtggc
    10621 agaagagctg cgtgccaacc actccggcga gctaatcgtg gcgcgttttg aggatgaaga
    10681 cgagtaacac actaatccgt atcgatttcg ttatagaagc cgatgatcat ttgattgtga
    10741 gaaaaaggac gctttttata tgtgattaaa agcgtcctga cccatttatg ctgatataaa
    10801 tttaactcag accagtgctt ttagttcttc ctgcatatat gttgaaaagt attcaggatt
    10861 tttaatcaca gagcgttcac ctgtctccat tttttttgcc catccaacca ttttttccgt
    10921 gaacccaggg ttccagcctt cctggtcagc tcgcgccgta atatcagcgg cactcgcggg
    10981 tactcccagt tcttttaaat atttaaagat tcccttcgcc gtgctttcat tcatcgaact
    11041 tggtgttggc gctgatacat ccatggcacc aatgaaatcc agggctttat ctattacagc
    11101 aggcatattc ttatccttaa aattaaccat gttatcgata aggctatata tgaccttaat
    11161 ttgcatctaa tatcaaaggg attataacga tatggcacga gttaacctgt tgacattcag
    11221 gtgttttaaa ggtagcagct catggtgtgt tatccagtaa gatggagaaa attaacggtc
    11281 agcccgcacg ctataaccag attgttcagc taagaaagaa aaccagagtt gctaatcaaa
    11341 gggcgaacac aacggcatgg ttatgtttat ttatatagtt agttttatag ataaaattga
    11401 aattataaat ataaataatg aattagcaga agcatcaaca gtaatatctt attctctggt
    11461 cgatttatgt aaaaccgacc agtattatcg ataaattatc gtctgactta ttttgcttcg
    11521 ataaagctta caccgcagcc acaatcttaa tttcaacttt atacttcggg ttcatcaaac
    11581 ccgcttgtac cgtgcagcgc accggcgcat gacccgcgac aacccaggca tcccacgctt
    11641 tattcatcgc cgcgaagtcg tttttatcgg ccaggaaaat ggtggcatcc agaatgctcg
    11701 atttattgct gccctgtttt tccagcacgg cgtcaatctg tgccagcgtg ttggcggttt
    11761 gctcaaaggc atcggcgtcg aggttttccg gtacaccagt gtagtagagc gtgttgttgt
    11821 ggattactac atcagaccag cgggcttcag catcgatacg aacgatagtc atcattttgt
    11881 cctcattata cttccatttt cgtgctgcaa agactgccac aatgtgaggt tgccgtcacc
    11941 acttcaactg gcgaaagcgc cctcatcctg acataatccc tgttcaaatc aacagggggt
    12001 agtgtgacgg acgattttgc accagacggt cagctggcga aagcgatacc aggctttaag
    12061 ccgcgagaac cacagcgaca gatggcggta gccgtcaccc aggcgataga aaaaggccag
    12121 ccgctggtgg tggaagcagg aaccggtacg ggcaaaacct acgcttacct ggctcctgcg
    12181 ctgcgggcga aaaagaaagt cattatctcg accggctcaa aagcgttgca ggatcagctc
    12241 tacagccgcg atttgccaac agtctcaaag gcattgaaat atacgggcaa cgtggcgctg
    12301 ctgaaagggc gctcaaacta cctctgcctc gaacgtctcg aacagcaggc gctggcgggg
    12361 ggcgatctgc cggtacaaat cttaagcgat gtgatcctgc tgcgctcctg gtctaatcaa
    12421 acagtcgatg gtgatatcag cacctgcgtc agcgtggcgg aagattcaca ggcgtggccg
    12481 ctggtcacca gcaccaacga caactgtctt ggcagcgact gcccgatgta taaagattgc
    12541 tttgtggtca aagcacgtaa aaaagcgatg gacgccgatg tggtggtggt aaaccatcat
    12601 ctctttctgg cggatatggt ggttaaagag agtggatttg gcgagttgat ccctgaagcg
    12661 gacgtcatga tcttcgacga agcccaccag ctaccggaca ttgccagcca gtattttggt
    12721 cagtcactct ccagtcgaca actgctcgac ctggcaaaag acatcaccat cgcctaccgc
    12781 accgaattaa aagacaccca gcagttacaa aagtgcgctg atcgtcttgc ccagagtgcg
    12841 caggattttc gtctgcaact cggtgagcca ggttatcgcg gtaacctgcg tgagctgtta
    12901 gctaatccgc aaattcagcg ggcattttta ctgctcgatg acaccttgga actttgttat
    12961 gatgtggcga aactgtcact ggggcgttcc gccttgctgg atgcggcatt tgagcgcgcc
    13021 acgttgtatc gcacacggct gaagcggcta aaagagatca atcagccggg ctacagctac
    13081 tggtacgaat gcacttcgcg ccattttact ctggctctca cgccgctcag cgtggcggat
    13141 aaattcaaag agttaatggc gcaaaagccc ggtagctgga tcttcacctc agcaacgctg
    13201 tcggtgaacg acgatctgca tcatttcacc tcgcggcttg ggatagaaca ggcggagtcg
    13261 ttgctattgc caagcccgtt tgattacagc cgccaggcgt tactctgtgt gccgcgcaat
    13321 ctgccgcaaa ccaaccagcc aggttctgct cgccagttag cggcaatgct gcgaccgatc
    13381 atcgaagcta acaacggtcg ttgttttatg ctttgtacct cgcacgccat gatgcgcgat
    13441 ctggccgagc agttccgcgc taccatgacg cttcccgttt tgttgcaggg ggaaaccagt
    13501 aaaggccaac tgttgcagca gtttgtcagt gccggtaatg cgcttcttgt ggcaaccagt
    13561 agtttctggg aaggggtgga cgtgcgtggc gatacattgt cattggtaat tatcgacaaa
    13621 ttgccgttta cctcgccgga tgatccactg ttaaaagcgc gcatggaaga ttgtcgtttg
    13681 cgcggtggcg acccgttcga tgaagtgcaa ctaccagatg ccgtcattac tctcaaacag
    13741 ggggtaggtc gactgattcg cgacgccgac gatcgtggcg tgctggtgat ttgtgactat
    13801 cggctggtga tgcgtcctta cggcgcgacg tttctcgcca gtctgccgcc cgcgccacgc
    13861 acccgtgaca ttgcccgtgc ggttcgtttc cttgcgatac catcctccag gtaatttgtt
    13921 acggagtatg gtatgatgcg cgccttttta ttgatctaag cacgaagacc catgcgaatt
    13981 ctggctatcg ataccgcgac agaggcctgc tctgtcgccc tgtggaacga cggtactgtc
    14041 aacgctcatt ttgagctttg ccctcgtgaa catactcaac gaatcttacc gatggtgcag
    14101 gatatcctga ccaccagcgg aacttccctg actgatatta acgctctggc ttacgggcgc
    14161 ggccccggta gctttaccgg cgtgcgcatt ggtattggca tcgcgcaagg gctggcgctt
    14221 ggcgcggaat taccgatgat tggcgtctcc acgctaatga cgatggcgca aggggcgtgg
    14281 cgcaaaaacg gcgcaacacg cgtgctgtca gccattgatg cgcgaatggg cgaagtttac
    14341 tgggccgaat atcagcgtga tgaaaacggt atctggcacg gtgaagaaac cgaagccgta
    14401 ctcaaacccg aactcgttca tgaacgaatg caacagcttt ccggcgaatg ggtgacggta
    14461 gggacgggct ggcaagcctg gccggatctc ggtaaagaga gcgggctggt tttgcgcgat
    14521 ggcgaagtgt tactccccgc tgcagaagat atgctgccga ttgcttgtca gatgtttgcc
    14581 gagggtaaaa cggtggcggt ggaacatgcc gagccggttt atttgcgtaa caacgtcgca
    14641 tggaagaaac ttccgggcaa agaatgaatc ttaagagtat gccctgagaa aaaaaggagt
    14701 cgcatcatgg cggttcaaaa gaatgttatc aaaggcatac tggcaggtac gtttgcgcta
    14761 atgctgagcg gttgtgtcac tgtgccggac gccattaaag gcagcagtac cacgccgcaa
    14821 caagatttag ttcgggtgat gagtgcgccg cagctgtacg ttggtcagga ggcacgcttt
    14881 ggtggcaaag tggttgcggt acaaaaccag caagggaaaa cccgcctgga aattgctacc
    14941 gtaccgctgg acagcggagc taggccgacg ctgggagaac cttctcgcgg tcgcatttat
    15001 gccgatgtga acggttttct ggacccggtg gatttccgtg gacaactggt tacggtagtc
    15061 gggccaatca ccggtgcggt tgacggcaaa atcggcaata cgccctataa atttatggtg
    15121 atgcaagtaa cgggttacaa acgttggcat ttaacccagc aggtgattat gccgcctcag
    15181 ccgattgatc catggtttta tggcggtcgt ggctggccct atggctacgg cggatggggc
    15241 tggtataatc ccggccccgc gagagtacaa acagttgtaa ccgaataatt gcttgttttt
    15301 aaagaatgca gaaacagcgg ctggtccgct gtttctgcat tcttacggta aagataaaaa
    15361 taaatagtga cgcgcttcgc aaccttttcg ttgggtaatt atcaagctgg tatgatgagt
    15421 taatattatg ttaacggcgt gtatatcatt tggggttgcg atgacgacga acacgcattt
    15481 tagaggtgaa gaattgaaga aggtttggct taaccgttat cccgcggacg ttccgacgga
    15541 gatcaaccct gaccgttatc aatctctggt agatatgttt gagcagtcgg tcgcgcgcta
    15601 cgccgatcag cctgcgtttg tgaatatggg ggaggtgatg accttccgca agctggaaga
    15661 acgcagtcgc gcgtttgccg cttatttgca acaagggttg gggctgaaga aaggcgatcg
    15721 cgttgcgttg atgatgccta acttattgca atatccggtg gcgctatttg gcattttgcg
    15781 tgccgggata atcgtcgtaa acgttaaccc gttgtatacc ccgcgtgagc ttgagcatca
    15841 gcttaacgat agcggcgcat cggcgattgt tatcgtgtct aactttgccc acactctgga
    15901 aaaagtggtt gataaaaccg ccgttcagca cgtaattctg acccgtatgg gcgatcagct
    15961 atctacggca aaaggcacgg tagtcaattt cgttgttaaa tacatcaagc gcttggtgcc
    16021 gaaataccat ctgccagatg ccatttcatt tcgtagcgca ctgcataacg gctaccggat
    16081 gcagtacgtc aaacccgaac tggtgccgga agatttagcc ttcctgcaat acaccggcgg
    16141 caccacaggt gtggcgaaag gcgcgatgct gactcaccgc aatatgctgg cgaacctgga
    16201 acaggtcaac gccacctatg ggccgttgct gcatccaggt aaagaactgg tagtaacggc
    16261 actgccgctg tatcacattt ttgccctgac cattaactgc ctgctgttta tcgaactggg
    16321 tgggcagaac ctgcttatca ctaacccgcg tgatattcca gggttggtaa aagagttagc
    16381 gaaatatccg tttaccgcta tcactggcgt taacaccttg ttcaatgcgt tgctgaacaa
    16441 taaagagttc cagcagctgg atttctccag tctgcatctt tccgcaggcg gtgggatgcc
    16501 tgttcagcaa gtggtggcag aacgctgggt gaaactgacc ggacagtatc tgctggaagg
    16561 ttatggcctg accgagtgcg cgccgctggt gagcgttaac ccgtatgata ttgattatca
    16621 tagcggtagc atcggtttgc cggtgccatc gacggaagcc aaactggtgg atgacgacga
    16681 caacgaagta ccaccgggtc aaccgggtga gctttgtgtc aaaggaccgc aggtgatgct
    16741 gggttactgg cagcgtccgg atgctacaga tgagatcatc aaaaatggct ggttacacac
    16801 cggcgacatc gcggtgatgg atgaagaagg attcctgcgc attgtcgatc gtaaaaaaga
    16861 catgattttg gtttccggtt ttaacgttta tcccaacgag attgaagatg tcgtcatgca
    16921 gcatcctggc gtacaggaag tcgcggctgt tggcgtacct tccggctcca gtggtgaagc
    16981 ggtgaaaatc ttcgtagtga aaaaagatcc atcgcttacc gaagagtcac tggtgacttt
    17041 ttgccgccgt cagctcacgg gatacaaagt accgaagctg gtggagtttc gtgatgagtt
    17101 accgaaatct aacgtcggaa aaattttgcg acgagaatta cgtgacgaag cgcgcggcaa
    17161 agtggacaat aaagcctgag cgttaagtca gtcgtcagac gccggttaat ccggcgtttt
    17221 ttttgacgcc cactaaagag aaaacaattt gaattaccaa atgattacca cggacgatgc
    17281 gctggcttct ttgtgtgaag ccgtccgtgt ctttccggcg atagccctgg atactgaatt
    17341 tgttcgtacg cgcacttatt acccgcagct ggggttgatt caacttttcg atggcgagca
    17401 tctggcgcta attgatcctc tcgggatcac cgactggtca ccgctgaaag cgatcctgcg
    17461 cgatccgtcc atcacaaaat ttctccatgc aggcagtgaa gatctggaag tgttcctcaa
    17521 tgtctttggc gaattaccac aacccttgat tgacacgcaa atccttgctg ccttctgcgg
    17581 acgcccgatg tcatggggtt tcgcttccat ggtggaagag tattccggcg ttacgctgga
    17641 caagagtgaa tcgcgcaccg actggctggc cagaccgctg accgaacgtc agtgtgaata
    17701 cgcagcggcg gatgtctggt atctgttacc gatcaccgcc aagcttatgg tagaaacgga
    17761 ggcctccggc tggctacctg cggcgctgga tgaatgccgc ctgatgcaaa tgcgtcgtca
    17821 ggaagtcgtt gcgccggaag atgcctggcg tgatatcacc aatgcctggc agttacgcac
    17881 acgccaactg gcctgtctgc aactgttagc cgactggcga ctgcgcaagg cgcgagagcg
    17941 tgatctggcg gtgaactttg tcgtgcgtga agagcatttg tggtcggtcg cgcgttatat
    18001 gccgggaagt ttaggcgaac tggacagcct gggtttatcc ggtagcgaaa tccgctttca
    18061 cggtaaaacg ctgctggcgc tggtggaaaa agcgcagaca ttgccggaag atgccttacc
    18121 gcagccgatg cttaacctga tggacatgcc tggttatcgt aaagcgttta aagcgattaa
    18181 gtcgctgatt actgacgtga gcgaaacgca taagatcagc gccgaattgc tggcatcgcg
    18241 tcggcaaatc aaccaactgc tgaactggca ctggaaactg aaaccgcaga acaatttgcc
    18301 ggagctgatt tccggctggc gtggtgagct gatggcggaa gcattacaca atttattgca
    18361 ggaatatccg cagtaaaatc ttccgaagcc ggactgggcg cgctcagcgc cacatccggc
    18421 tttggcaaac tacaaatcca acaccaggcg tttacccttc gcacgcgaac aacagatcaa
    18481 catactttgc tggctggcac gctcttcatc gctaaaatac tgatcccgat ggtcagcttc
    18541 accttccagt attgccgttt cgcaggttcc gcatacccct tcacgacata aacattccac
    18601 tttcgcggct ttattatttt caataacctg caaaatagtc atctcttccg gcaccacaaa
    18661 ctcttttccg gaacgggcaa gcaccagggt aaatgcatcg ccggttttgt cttcgatagc
    18721 aaattgctca aagtgcagtg tatcggcggc gatgtccaga cgcgcagctt cacttcttac
    18781 cgcttcaatt agcgcctcgg ggccacaggt ataaacgtgt gtgccaggtt cgatatccgc
    18841 caataatcgc gctaattcca gtcgtgttcc ggttgatgaa tgatgcaaat ggactttctc
    18901 agcctgcgga tgctggacta gctcagcacg aaatgcgcaa ctttctggat ttcgcgagca
    18961 gtaatgtagc tgccagtcga tgtcgctgtg ttgcaactct gccatgtgcg acaggaaagg
    19021 ggtgatacca ataccgccca cgataaacag atgctttctg gctgagggaa ttagcgcaaa
    19081 caggttatta ggcgttgaga tcgttaaccg atcgcccact tttacctgct gatgcaaaaa
    19141 gcgggaaccg ccgcgcgagt tttcttccag tcgaacggca atctgataac tagaggtgtt
    19201 atgcggtgaa ctcagtagtg aatacgcatt gctgtactgg ttatcaccat cgctcatctg
    19261 cactaggatg tgacttcctc cggtgaacgc aggcagcggt ttgccatcgg ttgccaccag
    19321 cgtgaagcgt ttcacctgtt cggtaagggg ttcaacctgg ctcacctgta cttcaaacat
    19381 ttgatagtct gacattgctg tactccatca aaaatcagcc cagcggcgga aaataccgcc
    19441 gccgatgtca ttagtcctta aacacctgcg ccagcagatt atggaaatgg gcgataccat
    19501 gttcggaaat gccactaccg ctactgtcgg ccatgatgcg cccctgacca cgatagccac
    19561 gcgatttcag ccctttctgt acgctttcaa ccagacgtaa atcttccgga tggaacacat
    19621 cgcgatacca ctcaatcagc gatttttgct cgtcggttaa ctcttcattg gtgaagtaaa
    19681 tatcgtagtt ttgcagggta gtttcagaat ccaccgggaa ttcataaatg accgtcatca
    19741 tccctttgat cggggtgacg ttcagcatcg tgcacggcca cagccagaaa ccgtggaatg
    19801 ccgcatccgt accctcttca aatttaaacg actgttcgga cggtttggcg aaaccgtatt
    19861 gcagcgtcca gttaccgtgc atggtgtgcc agtaacgatc aacctgtacg gagtcggaga
    19921 aacctagatg cgccggacca cagtgatagc actcgagata gttatcgaca atgttcttcc
    19981 agttggcagg cgtgcgggtg gtaaagcggg ccgctagttt cagattgtgg acttccgggc
    20041 aggcttccag cactttcgcg cccagaccgg tgtattgatt cacttgaagt acgaaaaaaa
    20101 accgggagga cattggatta ttcgggttct gatgggatta gatttggtgg ggcttgcaag
    20161 cctgtagcgc aaattttagt cgttaatcaa tgaaacgcga aagatagtaa aaaattgctt
    20221 ttgtttcatt gaaaatacga aaaacaaaaa cactgcaaat catttcaata acagcttcaa
    20281 aaaacgttca aaaccgataa caac
//
LOCUS       DAJTSI010000071        19902 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000071 DAJTSI010000000
VERSION     DAJTSI010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19902
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.071"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(3..1262)
                     /gene="rho"
                     /locus_tag="OO327_002276"
     CDS             complement(3..1262)
                     /gene="rho"
                     /locus_tag="OO327_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR6018804.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(1347..1448)
                     /gene="rhoL"
                     /locus_tag="OO327_002277"
     CDS             complement(1347..1448)
                     /gene="rhoL"
                     /locus_tag="OO327_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR6018805.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(1589..1918)
                     /gene="trxA"
                     /locus_tag="OO327_002278"
     CDS             complement(1589..1918)
                     /gene="trxA"
                     /locus_tag="OO327_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR6018806.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            2049..3314
                     /gene="rhlB"
                     /locus_tag="OO327_002279"
     CDS             2049..3314
                     /gene="rhlB"
                     /locus_tag="OO327_002279"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR6018807.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            3448..4932
                     /gene="gppA"
                     /locus_tag="OO327_002280"
     CDS             3448..4932
                     /gene="gppA"
                     /locus_tag="OO327_002280"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR6018808.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(4979..7000)
                     /gene="rep"
                     /locus_tag="OO327_002281"
     CDS             complement(4979..7000)
                     /gene="rep"
                     /locus_tag="OO327_002281"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR6018809.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            7087..7368
                     /gene="ppiC"
                     /locus_tag="OO327_002282"
     CDS             7087..7368
                     /gene="ppiC"
                     /locus_tag="OO327_002282"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR6018810.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(7414..8889)
                     /gene="ilvC"
                     /locus_tag="OO327_002283"
     CDS             complement(7414..8889)
                     /gene="ilvC"
                     /locus_tag="OO327_002283"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR6018811.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            9039..9929
                     /gene="ilvY"
                     /locus_tag="OO327_002284"
     CDS             9039..9929
                     /gene="ilvY"
                     /locus_tag="OO327_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR6018812.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMANQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(9926..11470)
                     /gene="ilvA"
                     /locus_tag="OO327_002285"
     CDS             complement(9926..11470)
                     /gene="ilvA"
                     /locus_tag="OO327_002285"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR6018813.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(11473..13323)
                     /gene="ilvD"
                     /locus_tag="OO327_002286"
     CDS             complement(11473..13323)
                     /gene="ilvD"
                     /locus_tag="OO327_002286"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR6018814.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSLPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(13388..14317)
                     /gene="ilvE"
                     /locus_tag="OO327_002287"
     CDS             complement(13388..14317)
                     /gene="ilvE"
                     /locus_tag="OO327_002287"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR6018815.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(14337..14600)
                     /gene="ilvM"
                     /locus_tag="OO327_002288"
     CDS             complement(14337..14600)
                     /gene="ilvM"
                     /locus_tag="OO327_002288"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR6018816.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(14597..16243)
                     /locus_tag="OO327_002289"
     CDS             complement(14597..16243)
                     /locus_tag="OO327_002289"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR6018817.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(16246..16296)
                     /locus_tag="OO327_002290"
     CDS             complement(16246..16296)
                     /locus_tag="OO327_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018818.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(16382..16480)
                     /gene="ilvL"
                     /locus_tag="OO327_002291"
     CDS             complement(16382..16480)
                     /gene="ilvL"
                     /locus_tag="OO327_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR6018819.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            16832..18311
                     /locus_tag="OO327_002292"
                     /pseudo
     CDS             16832..18311
                     /locus_tag="OO327_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(18336..18674)
                     /gene="maoP"
                     /locus_tag="OO327_002293"
     CDS             complement(18336..18674)
                     /gene="maoP"
                     /locus_tag="OO327_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR6018820.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            18793..19632
                     /gene="hdfR"
                     /locus_tag="OO327_002294"
     CDS             18793..19632
                     /gene="hdfR"
                     /locus_tag="OO327_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR6018821.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(19728..19803)
                     /locus_tag="OO327_002295"
     tRNA            complement(19728..19803)
                     /locus_tag="OO327_002295"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19768..19770),aa:Trp,seq:cca)
     gene            complement(19812..19888)
                     /locus_tag="OO327_002296"
     tRNA            complement(19812..19888)
                     /locus_tag="OO327_002296"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19852..19854),aa:Asp,seq:gtc)
BASE COUNT         4642 a         5312 c         5152 g         4796 t
ORIGIN      
        1 atttatgagc gtttcatcat ttcgaagaaa tcgtcattgg tcttggtcat ggccagttta
       61 ttaatgagga attccattgc atcgatttcg cccatcgggt gaatgatttt gcgcaggatc
      121 cacattttct gcagttcttc ctgagtcgtg agcagctctt ctttacgggt accggaacgg
      181 ttgtagtcga tagccgggaa gacgcgtttt tcagcgatct tacgagagag gtgcagttcc
      241 atgttgcctg tacctttaaa ctcttcgtaa ataacttcgt ccattttaga accggtatcg
      301 ataagcgccg tcgcgataat ggtcaggctg ccgccctctt ccacgttacg cgccgcacca
      361 aagaagcgtt tcggacgatg aagggcattg gcgtccacac caccggtcaa cactttacct
      421 gacgccggaa caacagtgtt gtaagcgcgc gccagacgag tgatggagtc gagcagaatg
      481 ataacgtctt tcttgtgctc aaccaggcgt ttggccttct cgatcaccat ttccgcaacc
      541 tgaacgtggc gagatgcggg ttcgtcaaag gtagaagcaa caacttcacc ttttaccaga
      601 cgctgcatct cggttacttc ttccggacgt tcgtcgatca gcagaaccat cagcacacaa
      661 tccgggtggt tgtaagcaat gctctgagca atgttctgca gcagcatagt tttaccggct
      721 ttcggcggtg ccacaatcag accacgctga ccacgaccga taggtgatgc cagatccagt
      781 acgcgagcag ttaaatcttc agtagaaccg ttaccacgtt ccatacgcag acgagagttt
      841 gcgtgcagcg gggttaagtt ctcaaagagg attttgttgc gggcgttttc aggtttgtcg
      901 aagttaactt cgttaacttt cagcagcgca aaatagcgtt caccttcttt cggcgggcga
      961 atcttaccag agatggtatc accagtgcgg aggttgaaac ggcggatttg gctaggggaa
     1021 acgtagatgt catcaggacc ggcgaggtag gagctgtctg cggaacggag gaaaccaaat
     1081 ccatcctgca atatctccag tacgccatca ccaaagatat cttcgccact ctttgcgtgc
     1141 tgcttcagga tggcaaaaat aatgtcctgc ttacgcatgc gagccaggtt ttccagcccc
     1201 atattttcgc cgagagtgat cagctcagaa accggcgtat tctttaattc ggtaagattc
     1261 atagtggtgt gagttcttaa acttggggta aatctcgaac ttaatgttgt gaatggtatg
     1321 gcaggatcat ccatgcctgt ttacggtcat cgactcatgt cttttcgctg cctggtcaca
     1381 ggagagtacg cagaactgaa acgacaagac ggattgagtg acgagccaga aatctgttca
     1441 cttcgcattt aagataaaac gggaagcgtt ggaaattaca agattcaaac ttaataaggt
     1501 atgtttaata cgaagtcaac actaagttag catgactcac gccgggcgtc cagtttttag
     1561 cgacggggca cccgaacatg aaattccctt acgccaggtt agcgtcgagg aactctttca
     1621 actgaccttt agacagtgcg cccactttgg ttgccgccac ttcaccgttt ttgaacagca
     1681 gcagagtcgg gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt
     1741 tcagttttgc aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg
     1801 cgatcatttt gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg
     1861 ctttgagtac atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat
     1921 ataactccac aggaataagc ctggcgtgtt ggtttcgttg ttggtgtaac attaaccaac
     1981 taaaggttga ctttatttca ccggatacgc tttcgtaaag caatagtaag ctgatattct
     2041 accacactat gagcaaaaca catttaacag aacagaagtt ttccgacttc gccctgcatc
     2101 cgaaggttgt agaagccctt gaaaaaaaag ggtttcataa ctgtacgccc attcaggcac
     2161 tggcccttcc gctgacgctg gcgggtcgtg acgtagccgg gcaggcgcaa accggtaccg
     2221 ggaaaacgat ggcgtttctt acgtcaacgt ttcattatct tctctctcat cctgcgattg
     2281 ccgatcgcaa ggtgaatcaa ccgcgtgcct taattatggc accgacgcgt gaacttgccg
     2341 tgcagatcca cgccgacgcg gaaccgctgg cggaagctac cggcctgaag ctgggcctgg
     2401 cttacggtgg tgatggctat gacaaacagc tgaaagtgct ggaaagcggc gttgacattc
     2461 tgattggcac cacgggtcgt ttaattgact acgccaagca gaaccacatt aacctcggtg
     2521 ccattcaggt agtggtactg gacgaagccg atcgcatgta cgatctaggc tttattaaag
     2581 atatccgctg gctgttccgc cgtatgccgc ctgcaaatca gcgcctcaac atgctgttct
     2641 ccgccacgct ttcgtaccgg gtacgtgaac tggcgttcga gcagatgaac aatgccgaat
     2701 atattgaagt ggaaccggaa cagaaaacgg gccaccgtat taaagaagag cttttctacc
     2761 cttctaacga agaaaaaatg cgtttgctgc aaacgctgat cgaagaagag tggccagaca
     2821 gagcgattat tttcgccaat accaaacacc gttgtgaaga gatctggggc cacctggcgg
     2881 cagatggtca tcgtgtcggt ttgttgacag gcgatgtcgc gcagaaaaaa cgtctgcgta
     2941 ttcttgatga atttacccgt ggcgatctgg atattctggt tgccaccgac gttgccgcgc
     3001 gtggtttgca tattccggca gtgacgcacg tctttaacta cgatttaccc gatgactgtg
     3061 aagattacgt tcaccgtatt ggtcgtacag gtcgcgcagg cgcaagcggt cactctatca
     3121 gcctggcgtg tgaagagtat gcattgaatt tgcctgctat tgagacctat attggtcact
     3181 caattccggt aagcaaatac aatccggacg cattgatgac cgatctgcca aaaccgctgc
     3241 gcctcacgcg cccgcgcaca ggcaatggtc cgcgtcgtac tggcgctccg cgtaatcgtc
     3301 gtcgttcagg ttaaataaaa tgtcatactc aaaatcattc aaactgtatc aagatggcaa
     3361 gagaatgaat cccgatgagc ttactgtagt aagtgattcg ggtgaaagaa tgcagccaac
     3421 acagagacag attgaaggat gaagagtatg ggttccacct cgtcgctgta tgcagccatt
     3481 gatctcggtt cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag
     3541 acgctgacgc gaattaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgac
     3601 ctctctaatg aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg
     3661 caagatatcc ctccctcgca aattcgcgtt gtcgctacgg cgacattacg acttgccgtc
     3721 aatgcgggtg attttattgc caaagcgcag gaaatcctcg gttgtccggt acaggtgatc
     3781 agcggtgaag aggaagcgcg tctgatttat cagggcgttg ctcacaccac tggtggtgcc
     3841 gatcagcgcc tggtggtgga tataggcggt gccagtactg aactggtaac cggcacgggt
     3901 gcacaaacca cctcgttgtt cagcctgtcg atgggctgcg tcacctggct ggaacgctat
     3961 tttgccgatc gtaatctggg gcaagaaaat tttgatgctg cagaaaaagc ggcacgcgaa
     4021 gtgttacgtc cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggtgct
     4081 tccggcaccg tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt
     4141 accctggaaa agttgcagca attgaaacag cgagccattc attgcggtcg gctggaggaa
     4201 ctggagattg acgggctgac gctggaacgt gcattagtgt tcccgagtgg tctggcgatc
     4261 ctgatcgcca tttttaccga actgaatatt cagtgtatga ccctggcggg cggtgcgctg
     4321 cgtgaaggcc tggtctacgg catgttgcat ctggccgtcg agcaggatat tcgcagccgt
     4381 acactgcgta atattcaacg ccgctttatg atcgatattg atcaggcaca gcgcgtagcc
     4441 aaagttgcgg ctaacttctt cgatcaggtg gaaaacgaat ggcatcttga agcaataagc
     4501 cgcgatttgc tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt tgacttcaaa
     4561 caagctccgc aacacgctgc ttatctggtg cgtaacctgg atcttcccgg ttttaccccc
     4621 gcacagaaga aactgctggc gacgctactg ctcaaccaga ctaacccggt cgatctctca
     4681 tcgctgcatc agcaaaatgc cgtaccaccg cgcgtcgcag aacaactctg ccgtttactc
     4741 cgcctggcta tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta
     4801 caggctaacc atgaactgtt gaccttgacg cttccgcaag gttggctaac ccaacatccg
     4861 ctgggtaaag agattattgc tcaggaaaac cagtggcaga gctatgtcca ctggccgctg
     4921 gaagtgcatt aatgagtaag tgccggatgc gatgctgacg catcttctcc ggccttgatt
     4981 atttccctcg ttttgccgcc atcatcgctt tcagattcgc cagatggctt tgccctttct
     5041 gcatccgttc ttcggcactg accactttgc gctcctgttc ccaaatcaga tcatcctgcg
     5101 gcagctccag caaaaagcgg ctcggctccg ggcgcaccag ttcgccgtac tgacggcgtt
     5161 ctttacacag cgtaaaggtc aattccttct gggcgcgggt aatgccgaca taggccagcc
     5221 gccgctcctc atcgatatta tcttcatcga tactgctctg gtgcggcaaa aacccttctt
     5281 ccataccgac catgtagaca taaggaaact ccagcccttt tgacgcgtgg agagtcatca
     5341 gttgcacctg atccagctct tcttcactct caccacgctc catcatgtcg cgtaaagtaa
     5401 agcgcgtcac cacctgggtg agcgtcatcg gctcatccag ttcgctgcct tccagcatct
     5461 ccgtcatcca gctaaacagt tggttgacgt tcttcatgcg catttcggca gctttcgtgc
     5521 tgggcgatgt ttcgtacagc caggattcat aatccatgcc gtggatcaga tcgcgcaccg
     5581 cggcaatcgg ctcccgctcc gccagacgct ggatttctgc caaccagtga gtgaagcggg
     5641 tcaatgcttc ataaccacgt ccgctaagcg tctgactcag gcccatatca aagctggcgg
     5701 taaacatgct tttattgcgc gtcatcgccc actcacccag ctttttcagc gtcgccgggc
     5761 caatctctcg cttcggcgtg ttaacgatac gcagaaatgc gctgtcatcg tccgggttag
     5821 tcagcacgcg cagataagcc agcaagtcct tgatttcagg acgagagaaa aacgacgtac
     5881 caccagaaat tttgtacggg atgcggtttt gcatcaggaa tttttcaaac acccgcgact
     5941 gatagttacc gcgataaaga atagcgtaat ctttgtactg cgttttattg acgaagtgat
     6001 gcgcaatcag ttcaccagtg acgcgctcgg cttcatgttc ttcgttattc gcgcttaaaa
     6061 cttttagctc tgtgccgtaa cccagttcgg agaacagacg cttttcaaag acgtgcgggt
     6121 tattggcgat caggatgttc gccgctttca aaatacgtcc ggaagagcgg tagttctgct
     6181 caagcttaat caccttcagc gccggaaaat cctggctcag cagcaccagg ttttgcggac
     6241 gtgcgccgcg ccaggagtag atcgactggt catcgtcacc caccacggta aagcgcgcgc
     6301 ggctgcccac cagcagtttc accagttcat actggctggt gttggtgtcc tgatattcgt
     6361 ccaccagcag gtagcgaatt ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca
     6421 gaagtagcgt cggcaataaa atcagatcat cgaagtcgag cacattacag gctttcaggt
     6481 gtgcatcata cagcccataa caatgggcaa aaatacggtc ccgctcgcca atcgcactgg
     6541 ctgccgcctg ggacggtgtt ttgagatcgt tcttccagtt agagatggtc gaaatcaact
     6601 gttgcaggag aactttgtca tcttcaatca gcccttcggt caactctttg agcaacgcca
     6661 gctgatcggt atcgtcaaac agcgaaaagt tcgccttcat ccccagcgcc gcatactcac
     6721 gcttgatgat atccagcccc agcgtgtgga aggttgagat catcagccca tgcgcctctt
     6781 tgcgacccag cgtctgtccg acacgctctt tcatctcgcg cgccgcctta ttggtaaagg
     6841 tcaccgccgc gatatgtcgc gcctgataac cgcacccgcg gatcagatgg gcgattttat
     6901 tggtgataac acgagtttta ccggaacccg cacccgccag caccaggcag gggccggtaa
     6961 cgaattcgac agcttgttgt tggccggggt ttagacgcat aggaaaatcg ctcaatgaaa
     7021 gtcgggaggg gaaaataaca gcgtggtagt atagccagcc tcatccttac cactcaaggc
     7081 acgatcatgg caaaaacagc agcagcactg catatccttg taaaagaaga gaaactggct
     7141 ctggatcttc tcgtgcagat taagaacggt gccgatttcg gcaagctggc gaagaaacac
     7201 tccatttgcc catcaggcaa acgcggcggt gatttaggtg aattccgcca gggtcagatg
     7261 gttccggcgt tcgataaagt tgtgttctct tgtccggtac tggagccaac cggcccgctg
     7321 cacacccagt tcggttacca catcattaag gtgctgtacc gtaactaata gcaaggcctt
     7381 ctccgggaga aggccttgaa ctctgaatcg ctattaaccc gcaacagcaa tacgtttcat
     7441 atccgtcata tagccgcgca gtttcttacc tacttgctca atcgcatggc tgcgaatcgc
     7501 ttcatttaca tcacgcagct gcgcgttatc taccgcacct tccggaatag ctttacccaa
     7561 atcgcccggt tgcagttctg ccataaacgg tttcagcagc ggcacacaag cgtaagagaa
     7621 cagatagtta ccgtactcgg cagtatcaga gataaccacg ttcatttcat acagacgctt
     7681 acggacgatg gtgttggcaa tcagcggcag ttcgtgcagt gattcatagt aagcagattc
     7741 ttcgatgatg ccggaatcaa ccatggtttc gaacgccagt tcaacgcccg ctttcaccat
     7801 cgcgatcatc agtacgcctt tatcgaagta ctcctgctcg ccgattttgc cttcatactg
     7861 cggcgcggtt tcgaaggcgg ttttgccggt ctcttcacgc caggtcagca gtttcttatc
     7921 atcgttggcc cagtccgcca tcataccgga ggagaattcg ccggagatga tgtcgtccat
     7981 atgtttctgg aacagcggtg ccatgatctc tttcagctgt tcagacaacg cgtaagcacg
     8041 cagtttcgcc gggttagaga gacggtccat catcagtgtg ataccgccct gcttcagcgc
     8101 ttcggtgatg gtttcccaac cgaactgaat tagtttttct gcgtatgccg gatcggtgcc
     8161 ctcttccacc agcttgtcga agcacagcag cgagccagcc tgcaacatac cgcacaggat
     8221 ggtttgctca cccatcaggt cagatttcac ttccgcaacg aaggacgatt ccagcacgcc
     8281 cgcacggtga ccaccggttg ctgccgccca tgctttagcg atcgccatgc cttcgccttt
     8341 cggatcgttt tccgggtgaa cggcaatcag cgtcggtacg ccgaatccac gtttgtactc
     8401 ttcacgcact tcggtgccag ggcatttcgg cgcaaccatg actacggtga tgtctttacg
     8461 gatctgctcg cccacttcga cgatgttgaa accgtgcgag taacccagcg ccgcgccgtc
     8521 tttcatcagt ggctgtacgg tgcgcactac atcagagtgc tgcttgtccg gcgtcaggtt
     8581 aaccaccaga tccgcctgtg ggatcagttc ttcgtaagta cccactttaa aaccattttc
     8641 ggtcgcttta cgccaggacg cgcgcttctc ggcaatcgct tctttacgca gagcgtagga
     8701 gatatcgaga ccagaatcac gcatgttcag accctggttc agaccttgtg cgccacagcc
     8761 gacgatgact acttttttac cctgaaggta gctcgcgcca tcggcgaatt catcgcgccc
     8821 cataaagcga catttgccca gctgtgccag ctgctgacgc agattcagtg tattgaagta
     8881 gttagccatg gtgattcctc gtgatgttgt gcttcttatt gttcggttcg cgtttgcgag
     8941 attgaattca ctatatgaca ggaaatttat tgcggaaatt gatatattca caacgtcaca
     9001 ttgcaatttc tgcaacgtca acattgaggg ctgcgccatt ggatttacgc gatctgaaaa
     9061 ccttcctgca tctggcggaa agccgccatt ttggccgcag cgcgcgggcg atgcacgtta
     9121 gcccgtccac gctctcacgg cagattcagc gcctggaaga agatctcggc cagccgctgt
     9181 ttgtgcgcga taaccgcacg gtgacgctga ctgaagcggg cgaagaactg cgcgttttcg
     9241 cccagcaaac gctgttgcag tatcagcagc tgcgccacac catcgaccag caagggccgt
     9301 tgctctatgg cgaattacat atcttctgct cggtgaccgc cgcttacagc catctgccgc
     9361 cgattctgga tcgcttccgc gcggaacacc cgtcggtgga gatcaaactc actaccggtg
     9421 atgcggcaga tgcaatggaa aaagtcgtca caggtgaagc tgatctggcg attgccggga
     9481 agccagagac tctgcccggc gcggtggcgt tttcgatgtt ggagaatctg gcagtggtgt
     9541 tgattgcacc cgcattgccc tgcccggtgc gtaatcaggt ttctgtcgaa aagccggact
     9601 ggtcaacggt accgtttatc atggccaatc aggggccggt gcgccgccgc attgagttgt
     9661 ggttccgacg caataaaatc agtaacccga tgatttacgc cacggttggc gggcatgaag
     9721 cgatggtatc gatggtggcg ctcggctgtg gcgtggcatt gttgccggaa gtggtgctgg
     9781 aaaacagccc cgaaccggtg cgtaaccgcg tgatgatttt agagcgcagc gatgagaaaa
     9841 cgccgtttga gctgggcgtg tgtgcgcaga aaaagcggct acatgagccg ctaattgagg
     9901 cattctggaa gattttgccg aaccattaac ccgccaaaaa gaacctgaac gccgggttat
     9961 tggtttcgtc gtggcaatcg tagcccagct cattcagccg ggtttcgaaa tccggttcat
    10021 ggtcgccaag ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt
    10081 ggaacaaaga aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg
    10141 gtgattccgg gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc
    10201 cgaccatata gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt
    10261 agccgccgtc gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc
    10321 gcacgccgac aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga
    10381 ccgatcgccc gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg
    10441 tcaccgccaa caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca
    10501 ggccgtggaa gttcacgttg gcaccggaaa gaatatgcgc cagccgttcg ccgcgaatgt
    10561 tgtgctgggc gatatatttt ttcattcccg ccagcgccag cgcgccagag ggttccgcca
    10621 ccgcacgcac atcttcgaat aaatccttca tcgccgcaca gatcgcatcg ctatcgacgg
    10681 tgatgatgtc gtcgagatac tcctggcata aacggaaggt ttcgtcaccg atgcgtttta
    10741 ccgcgacacc ttcggcgaac agtcccacgc gtggcagatc aaccggatga cccgcatcca
    10801 gcgctgcttt caggcaggcg gagtcttccg cttctacggc gatcactttg atttgcggca
    10861 tcagttgttt gatcagcacc gctacgcccg ccgccagacc gccgccgccg actggcacaa
    10921 atacgcggtc gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg
    10981 caatcaccat cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt
    11041 cgatcgcttt agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga
    11101 atccacgtac cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct
    11161 tcacgcctaa ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag
    11221 tgatcacgcc gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc
    11281 gcagcttaaa gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa
    11341 gacgcgacga cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg
    11401 gcgcgcgcag cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg
    11461 agtcagccat tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct
    11521 ggttgccagg ctggcgtagg cgcacagggc aaaggaaacc tgacgttcac ggtttttcgg
    11581 cgtccaggct ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc
    11641 gcttacctgt aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc
    11701 aatcagacca atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc
    11761 agaggtgccg ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat
    11821 tgatttcagg aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc
    11881 ttcatagcgg atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac
    11941 cgcgtcgtcc tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc
    12001 gacgcccgcg gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag
    12061 gccgccgtct ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc
    12121 atcgagagaa tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg
    12181 gcctgcgcgg aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc
    12241 cagagtttgc ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc
    12301 gcgatccagt tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg
    12361 gtatttttgg gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc
    12421 gatatcactc atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac
    12481 cgtgttggta gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt
    12541 gctagcaata ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa
    12601 ttcaacaatg cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag
    12661 cagcgagccg ttgcccggca gcgacaggcc cagcgcttcg gtcaggcagt tcattgagtt
    12721 agcggtaaac atcccggagc aggagccgca ggtcggacac gcggaacgtt caacctgatc
    12781 gctctgggag tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag
    12841 tttgattatc tgatcggaca gtttggtttt cccggcctcc atcgggccgc cggaaacaaa
    12901 gatcaccgga atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca
    12961 gttagagatg cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc
    13021 agcgatcagt tcgcgagatg gaagtgaata aagcatcccc ccgtggccca tggcaatccc
    13081 atcatccacc gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc
    13141 ggcgaccagt ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga
    13201 gttcacaacc gcgataatcg gcttgccaaa atcggcgtcg gtcattccgg tggcgcgcca
    13261 cagcgcacga gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg
    13321 catactttat ttactcccag tgtctgtctc gtaaatggga cggtgcgtgc cgtcccattt
    13381 tttgtattta ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga
    13441 agaggccgaa gaaggcttgc tgaatgcgtt tggtaaccgg gccacaacgg ccttcgccaa
    13501 tctgaatacc gtctacgctg cgcactggcg tgatttctgc cgccgtaccg gacataaaga
    13561 cttcatccgc caagtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca
    13621 gctctttcgc cagtttgatg atggcatcac gggtaatacc cggcagcgcg gaggaggtga
    13681 acggtggggt gaacagcaca ccatctttca cttcaaacag gttttcgcct gcgccttcag
    13741 agatataacc gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc
    13801 tacccaccag cagggaagag agatagttac caccggcttt tgccgcagtt gggatggtgt
    13861 ttggtgctgc gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg
    13921 cgcccagata cgctccccac gggaaagcgg cgataatcac atcggttgag tatcccgctg
    13981 gcgggtttac tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg
    14041 tgagattgtt tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgtg
    14101 aaaccgggaa gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc
    14161 ggaatacaac cggtcctttg tgtgagtcgt agcaacggat gccttcaaaa accgaggtgc
    14221 catagtgcag cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc
    14281 cattgaacca aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag
    14341 gcgcggattt gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc
    14401 agtttattta actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg
    14461 atatttatat tttgtgcatc gctggcggca gccatattca ttgagcagac gtggaaacca
    14521 cgatgacgca ccacgcgtaa aacacgttct aaggtttccg gattgaagcg agccgataca
    14581 ttgacctgat gttgcatcat gataatttct ccaacatttc tgaattactg gcacctggcg
    14641 gcaccagcgg ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat
    14701 cactgttcag catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc
    14761 cagggatgcc gaaggcgctg gctaacatga ggaaatcggg gttatcggta agggtggttt
    14821 cgctgtatcg ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt
    14881 tatcgagtaa gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca
    14941 cattcatcat gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga
    15001 cttgtgcgcc aactgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga
    15061 tgaaattttc cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca
    15121 catctgtggt cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg
    15181 gagcgtagat agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg
    15241 cgcagtgttg ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat
    15301 ttaaatcacc ttgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga
    15361 tatccatatg gataacactg gcgtgtggtg cgaaggtgtt cagtttgccg gtcacccggt
    15421 catcaaaacg tgcacccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg
    15481 ctttggttcc gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc
    15541 ccagcccttt cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag
    15601 ccggaactgc ctgcgccatc cccacgccac cgccaacgta cagcatcggt ttttgcgctt
    15661 ttgccagcat ctggcgcgct tgctcgactt cggcatgtgg gaaagtcact tcgttttcaa
    15721 cggtggtgaa ccacggttcc agatcgccgc tggctaactg gatatctttt gggatatcga
    15781 ccagaaccgg accaggacga cctgagctgg caacgtcgaa tgcttcagcc atgatgcgcg
    15841 gcaactcttc cagagactgc accagaaagc tgtgcttggt acaggctaac gacaatccca
    15901 ggacatccac ttcctgaaat gcgtcggtgc cgataaacgg tgcggacacc tgaccagtga
    15961 tggcaacgac agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg
    16021 cgcccggacc agacgtggcg atacatacgc cagttttacc ggtagcacgg gcataaccga
    16081 tagccgccat tgccgcaccc tgctcatgtc ggcacagcaa gtgctccacg ccgccgtcat
    16141 acaatgcatc gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac
    16201 cctgtgcccg caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct
    16261 gaatcttgag aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt
    16321 aaggtcaaaa aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc
    16381 tctaagcctt tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc
    16441 taatcacgac caggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt
    16501 gttcgaagga atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg
    16561 gcctattttt atgatggctt taataatttc aatagaaatt gttgtttttt aaaaagaaaa
    16621 tagtgagtga aaaaattcaa tttcagtata aatgttgttc ccttcctcgt agttcatcct
    16681 ttgtccgctg catttttgcg agcatcattc caccgccaga acaactcaac ttcacttgca
    16741 caaataaaca ggaatcagcc actaaaattc acgaattagc gggctacagc agcgatatct
    16801 tatgcacata atggtgactc aaggagggtt tatgtcactg tcaattgttc atacccgcgc
    16861 agccctggaa gtaaatgcgc ccccaatcac tgttgaggta catatcagta aaggtctacc
    16921 cggcttaacg atggtgggct taacagaaac aacggtgaaa gaagctcgcg atcgcgtgcg
    16981 cagcgccatt atcaatagcg gatatgaata tccggcgaaa aaaatcacca tcaaccttgc
    17041 tccagccgat ctgccaaaag aagggggacg atatgattta cctatcgcca ttgcgttgct
    17101 ggcggcctca gaacagctta cagccaataa gttagatgaa tatgaattag tcggagaact
    17161 agcgcttaca ggcgctctgc gtggcgttcc cggtgcaatc tccagtgcaa ctgaagctat
    17221 taagtcgggc agaaaaatta tcgtcgcgaa agataacgaa gatgaagtgg ggctaattaa
    17281 cggtgaagga tgcctgatag ccgatcatct gcaggctgtc tgtgcgtttc tggaaggtaa
    17341 gaacactctc gaacgcccga aaccaactga tgcagtatcc cgggcgctac aacatgatct
    17401 cagtgatgtt gtcggtcagg agcaaggaaa acgaggactg gaaattaccg ccgctggtgg
    17461 gcacaacctt ttactgattg ggccgccggg aacaggtaaa acaatgctcg ccagccgtat
    17521 taatggcctg ttgccagatt taagcaatga agaggcactg gagagcgctg cgatattaag
    17581 tctggtaaat gctgaatcag tacaaaaaca atggcggcag cgcccgttcc gctcacctca
    17641 tcacagtgcg tcgttaactg cgatggtggg cggtggtgca attccagggc caggtgaaat
    17701 ttcgttggcg cataacggcg tgctttttct tgatgagcta cctgaatttg aacggcgtac
    17761 actggatgcc ttgcgagagc cgattgaatc cgggcagatc catctttcac gcacacgagc
    17821 aaaaataacc tatccagccc gtttccagct tgttgcggcg atgaatccca gccctaccgg
    17881 acattatcag ggaaaccata accgctgcac gccagaacag acattacgtt atctcactgg
    17941 agatcccatt accacccccc ggcattttga gtaaaacggt agtgccggga gaaagcagcg
    18001 ccaccgttaa acaacgcgta atggccgcca gagagcgcca atttaagcgg cagaataaac
    18061 tgaatgcctg gctggatagt ccggaaatac gccaattctg caaacttgag agcgaagatg
    18121 cgatgtggtt ggaagaaacg ctgatccatc tggggttatc gattcgtgcc tggcagcggt
    18181 tactgaaagt tgcacgaacc attgctgata ttgatcagtc tgacattatc acacgtcagc
    18241 atttgcagga ggcagttagc tatcgtgcga ttgaccgttt gctcatccat ctgcaaaaac
    18301 tactgacata aaaaaagggc atttcgccct ttttattaat cgtcagaatc ggtgtagtct
    18361 tcagcacctt caacctgcgg tttaccgccg gaaagggtgt gaaaacgttt tggacgctta
    18421 atacgcgtca tatacttgga ccacacgcgt tctgcttctg tcactggctc acgttcgcca
    18481 cggcatactg ctacgaagag tttctcttcc tcggtaaccg gctcgcgttt gccaagatcc
    18541 aactcattga aggcataacc atgacgctca agcagttgtg cctctttgat ggtgaaatca
    18601 ccatgacgag agaatccacg tggataatgt ttattgtcga aatatcgatt agtcgtcgta
    18661 aagctttccg ccatcctgca cgctcctaat tctttgaccg agctagttat ggcgcggagt
    18721 attagttacg cttgacagag tgtaaaacaa aacatttaaa tcataacgac aaataatttt
    18781 gcggagagca ctgtggatac ggaattgtta aaaactttcc tggaagttag ccgaacgcgt
    18841 cactttggtc gagcggctga atcgctctat ctgacccagt cagcagtgag ctttcgaatc
    18901 agacaactgg aaaatcaact gggtgtgaac cttttcaccc gccacagaaa caatatccgt
    18961 ttaaccgctg ccggtgaaaa actactgcct tatgcagaaa cgctcatgag cacctggcag
    19021 gccgcccgca aggaggtggc gcatacctca cgacataacg agttttctat cggtgccagc
    19081 gcctcgttgt gggaatgtat gcttaatcag tggttgggac gcttgtatca aaatcaggat
    19141 gcccatacag gcttacagtt cgaagcgcga attgcccaac ggcagtctct ggtaaaacag
    19201 ctgcatgaac gccagcttga tcttcttatt accactgaag cgcccaaaat ggacgaattt
    19261 agtagtcagt tgctggggta tttcacttta gcgctttata ccagtgcccc ttctaaacta
    19321 aagggagatc ttaactatct gcgactggag tgggggccag attttcaaca gcatgaggca
    19381 ggtttgatcg gtgctgacga agttcccatt ctgacaacca gttctgctga actggcacag
    19441 caacagattg cgatgcttaa tggttgcacc tggctacccg tcagctgggc gcgtaaaaaa
    19501 ggcggcctgc ataccgttgt cgatagcaca acactttcac ggccgcttta tgccatatgg
    19561 ctgcaaaata gcgataaaaa tgcgttgatt cgcgatcttt tgaaaattaa cgtgctggat
    19621 gaagtgtatt aatctgaatg gctggcaagg atgccggtag aaggatttac ttcggagagg
    19681 attatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg caggggcgga
    19741 gagactcgaa ctcccaacac ccggttttgg agaccggtgc tctaccaatt gaactacgcc
    19801 cctaattagg gtggcggaac ggacgggact cgaacccgcg accccctgcg tgacaggcag
    19861 gtattctaac cgactgaact accgctccac cgaattcttt ta
//
LOCUS       DAJTSI010000072        19886 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000072 DAJTSI010000000
VERSION     DAJTSI010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19886
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.072"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            154..354
                     /locus_tag="OO327_002297"
     CDS             154..354
                     /locus_tag="OO327_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR6018822.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            344..565
                     /locus_tag="OO327_002298"
     CDS             344..565
                     /locus_tag="OO327_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018823.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            558..778
                     /locus_tag="OO327_002299"
                     /pseudo
     CDS             558..778
                     /locus_tag="OO327_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            780..1013
                     /locus_tag="OO327_002300"
     CDS             780..1013
                     /locus_tag="OO327_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018824.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            1019..1318
                     /locus_tag="OO327_002301"
     CDS             1019..1318
                     /locus_tag="OO327_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR6018825.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            1315..3063
                     /locus_tag="OO327_002302"
     CDS             1315..3063
                     /locus_tag="OO327_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6018826.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVVIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            3352..3630
                     /locus_tag="OO327_002303"
     CDS             3352..3630
                     /locus_tag="OO327_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018827.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            3627..3932
                     /locus_tag="OO327_002304"
     CDS             3627..3932
                     /locus_tag="OO327_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018828.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWE
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            3942..4103
                     /locus_tag="OO327_002305"
     CDS             3942..4103
                     /locus_tag="OO327_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR6018829.1"
                     /translation="MTAQIAAYGQLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            4407..5564
                     /locus_tag="OO327_002306"
                     /pseudo
     CDS             4407..5564
                     /locus_tag="OO327_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            5604..6176
                     /locus_tag="OO327_002307"
     CDS             5604..6176
                     /locus_tag="OO327_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR6018830.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            6178..7389
                     /locus_tag="OO327_002308"
     CDS             6178..7389
                     /locus_tag="OO327_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR6018831.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            7386..7724
                     /locus_tag="OO327_002309"
     CDS             7386..7724
                     /locus_tag="OO327_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR6018832.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            7721..8017
                     /locus_tag="OO327_002310"
     CDS             7721..8017
                     /locus_tag="OO327_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCR6018833.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            8017..8457
                     /locus_tag="OO327_002311"
     CDS             8017..8457
                     /locus_tag="OO327_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6018834.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            8441..8623
                     /locus_tag="OO327_002312"
     CDS             8441..8623
                     /locus_tag="OO327_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018835.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            8679..9005
                     /locus_tag="OO327_002313"
     CDS             8679..9005
                     /locus_tag="OO327_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR6018836.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            8989..10650
                     /locus_tag="OO327_002314"
     CDS             8989..10650
                     /locus_tag="OO327_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR6018837.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            10664..10945
                     /locus_tag="OO327_002315"
     CDS             10664..10945
                     /locus_tag="OO327_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018838.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(11742..13202)
                     /gene="phoQ"
                     /locus_tag="OO327_002316"
     CDS             complement(11742..13202)
                     /gene="phoQ"
                     /locus_tag="OO327_002316"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR6018839.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(13202..13873)
                     /gene="phoP"
                     /locus_tag="OO327_002317"
     CDS             complement(13202..13873)
                     /gene="phoP"
                     /locus_tag="OO327_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR6018840.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(14042..15412)
                     /gene="purB"
                     /locus_tag="OO327_002318"
     CDS             complement(14042..15412)
                     /gene="purB"
                     /locus_tag="OO327_002318"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR6018841.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(15416..16057)
                     /gene="hflD"
                     /locus_tag="OO327_002319"
     CDS             complement(15416..16057)
                     /gene="hflD"
                     /locus_tag="OO327_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR6018842.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(16093..17199)
                     /gene="mnmA"
                     /locus_tag="OO327_002320"
     CDS             complement(16093..17199)
                     /gene="mnmA"
                     /locus_tag="OO327_002320"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR6018843.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(17253..17714)
                     /gene="nudJ"
                     /locus_tag="OO327_002321"
     CDS             complement(17253..17714)
                     /gene="nudJ"
                     /locus_tag="OO327_002321"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR6018844.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(17724..18377)
                     /gene="rluE"
                     /locus_tag="OO327_002322"
     CDS             complement(17724..18377)
                     /gene="rluE"
                     /locus_tag="OO327_002322"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR6018845.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            18549..19799
                     /gene="icd"
                     /locus_tag="OO327_002323"
     CDS             18549..19799
                     /gene="icd"
                     /locus_tag="OO327_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR6018846.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
BASE COUNT         5186 a         4874 c         5304 g         4522 t
ORIGIN      
        1 cctttcgttt ttgtgtgttg acacgttaat tacacctcag gtaatgtatt tatgtgtagt
       61 gacacattac acacaatcaa cacaaaaagc aaagccccgc actgtaggag cagtaacggg
      121 gcttctaacc aacaacgtaa actaggagcc gttatggttg ccgtaaatca tataccacac
      181 cttgtacaca cacaaacggc ctttgtgtgg cgttttctgg cactgagtgc cggagaatcc
      241 caaatcatcc acgtaaccgc ttggacggaa cgcgaagcgc gtagccgttg cccgtccggt
      301 tgtgttgctg tattcgccgc ccgtattcgt cagggggaaa cctatgcaca ataaaaccac
      361 accgaacgca gcagccgccg cactcactac gctgatgcac gcgcttattg atattgaatg
      421 cacggcagag cttgcgcagg gagaagaaca gaaagaccgg acacagttcg ccctggaatg
      481 tatccgatac atcgcaacgc ggtcactgaa tgacgctaaa aatattcttg ttgccgattg
      541 tgaaaatggg gggggttatg cgtgatgatc gttttaattc cctgaaacag gaattttccg
      601 gcgttcctga tgatgcggct gatgcgcttt cgtcaatgcc agaacttatt agagcggctt
      661 ttttcttact ttccacgaga gaatataaat caacgggctt gatgtactga atatcgccgc
      721 cgattatgct gatttcgtga cagaggttat tttaagaaaa acgacggacg gggattaata
      781 tgcgtgatat ttaccacgaa acaatagacc gcgcatttct tgcactttct cacagtgaaa
      841 acatgctgga aatattgcgc atatggcttg aaacacttgg cgacaatgaa cgcgacaaac
      901 aaaaatcaag aattgccacg gcattaataa cgcttcttga gcctgtaata atggaactgc
      961 aagaaataga tctattgcac gacagatata aagaacaaca caccggagaa taaaaataat
     1021 gaaacttaaa tattctggct taactgccag tggcaacact caccctaaat ttacgcgcgg
     1081 tgatatttac cgcgaccagt acggcggcat ggtaacgatt aaaggcgtgg cagaacggcg
     1141 catcacctac cgccgtgaag gttacgaata tgattgcgtg atgcctgttt atcaattccg
     1201 gcgtgatttc acgctggtac aggcagcgcc ccgcagtaaa ccaaccagca gggagaaagc
     1261 acgcgccaat attcaggaaa taaaaaagat gcttaacgta ttcaggggta aaaaatgaaa
     1321 ctggcaccga acgtaaaaaa acagccacgc ggaataaaac acaaagacac agaggtaatt
     1381 atctttgcgg gtagtgatgc ctggtcgcac gcgaaacaat ggcaggagca ggatggcccc
     1441 gcatccggcg ataatgtgcc gcctgtgtgg cttgggccaa atcagcttgc cgaacttgat
     1501 gcactgaaaa ttgttccgga tggaaaaaaa cgcgtaaggc tgtaccaggc cggagaactg
     1561 gatttggtgg agaccaaaaa gattggtcag aagctggcgg cggcagatat tcaggacgca
     1621 aatttttacc ccgaaggaat gcacgtccag aagtgtgaaa actggcggcg ctatctgaat
     1681 gctgagcgtg aaaatattgc cgcagggctt accatgccgg agcagaaaaa tacgcaactg
     1741 gcacaaatgg cagacagtga gcgcgcgcag ctgcttgccg agcgctttga tggcgtttgt
     1801 gtgcatcagg aaagtgaaat cgttcacgta tggcgcggcg gggtatggtg tccggtcagc
     1861 acaatggaac ttagccgcga aatggtggtg atctattcag agcacagggc cactttcagc
     1921 aagcgcgtaa tcaataacgc cgtggaagcg ttaaaagtta ttgccgaacc aatgggcgag
     1981 ccgtccggcg atttgctgcc gttcgccaat ggtgcgcttg acctgaaaac gggggaattt
     2041 tccccgcaca cgccggagaa ctggatcacc acgcacaacg gcattgagta cacgccacca
     2101 gcaccagggg agaacatccg cgataacgcg ctaaactttc ataaatggct tgagcacgca
     2161 gccggaaaag accagcgcaa gatgatgcgt atatgtgccg cgctgtacat gattatggcg
     2221 aaccggtacg actggcagat gtttattgag gccaccggag acggcgggag cggtaaaagt
     2281 acattcacac acatagccag ccttctggca gggaaacaga acacggtaag cgctgaaatg
     2341 acatcgcttg atgatgctgg tggacgtgcg caggttgtcg ggagtcgtct tatcgtcctg
     2401 gctgaccagc cgaaatatac aggcgaagga acgggcatca agaaaatcac gggcggtgac
     2461 cccgtggaaa ttaacccgaa atacgaaaag cgtttcacgg ctgtaatcag ggcggtggtg
     2521 ctggcgacca acaataaccc gatgatattc accgaacggg ccggaggtgt ggcacgtcgt
     2581 cgcgtgattt tccgtttcga caatatcgtt agtgaggccg aaaaagacag ggagctaccg
     2641 gaaaagattg cggctgaaat ccctgtcatt atccgccgat tgctggcgaa ctttaccgac
     2701 tctgagaaag cacgggcttt actactggaa cagcgtgacg gtgatgaagc tctggcaata
     2761 aagcaacaga cggatccggt tattgagttt tgccagttcc tgaattttct ggaggaagca
     2821 cgcggcctga tgatgggcgg cggtggcgat tcagtgaagt acacgaccag aaacagcctt
     2881 taccgcgtct atctggcgtt tatggcgtac gcaggcagga gcaaaccgct aaacgtaaat
     2941 gactttggca aggctatgaa gccagccgcg aaagtttacg gacatgaata tattacgcgg
     3001 aaagttaagg gagtaacgca gactaacgca ataacaacag acgagtgcga cgcgttttta
     3061 taattttttg taattgctgt ctaccctgtc tacctgagta aagaaaaata catttaattc
     3121 agtgcattaa gttaggtaga tagccttttt ttactgtcta cccactatct accctctcta
     3181 cctgatttta cctgaatcag acagggaggt agacacgggg tagatagtgg ataaaagcac
     3241 tctaccccgc tgaaagcagc gccattactg gcatgagggc cactaaggta gataaggtag
     3301 acaaggggag gcacaactca aaacttttta aacgaggggg taaaaataaa tatgcacact
     3361 tcagggaaat ttaataaatc attcaaaaaa cgcagagaca gaacagaacc gaaatatcgc
     3421 gcgttagaca tgacagagca cgctttaaag gtggcaatca gaacgataga ccgccacgcg
     3481 ggggaaggat acgcgaaagc acatcccgaa ctgataagcg cattcatgac cacgacggcg
     3541 gcaaattttg ccacgctgac agagcgggag attgccgaag cggaacaggt aacaacaatc
     3601 aacgttaaaa ccggagaggt ggaatcatga gcgaaccaaa atttggtgaa aaattttaca
     3661 agcacaacgg acggatcacg attctgcaaa taagcgcagc tacaccaggc tggtgggttg
     3721 aaacagacga aggatcctca cccgtggcat catgggagct ttgtgtgctt agttaccctg
     3781 accgcgatgt gtatcaggat attctgcctg tgatatccac tgacaaaggc atgaaacccg
     3841 tagacatcaa aaacatgggg tttcagtgcg taatgctcac tgaaaaaatg atggaagaga
     3901 tgaaaaagaa caatgccgga tcgcttcact gaggtggcaa tatgacagca caaattgcag
     3961 cttacgggca gctggtgggc gacccgctgg taaaacagag cagcaagggc acaccggagc
     4021 cgccaacgga gaactacgca gacctgagaa atgcagtaaa tcgcacttac gcagacatgg
     4081 ggctgagcaa attagcagag taggaatatc agaaccacac cacgcagccg gagcaatccg
     4141 gctttttgtt attttttgta aattatttgt tcgtggttgt tccacgttgt tcactgagcg
     4201 gatcggcata ttttacccga actgaatcat gattattctc gcccgtggtg ccaggacgct
     4261 ggggccactt tcccgcctgt taatgtgctc gccaatattc attaccaggc gggaaaacga
     4321 tcggtgcgat tactgatttc cttatgaaaa acggttgagt gtttgccgcg tcctggagtt
     4381 ccttacttaa ccccaggact ttttttatgc agagaataat cgaattacgc cagcagaaaa
     4441 ccgccattaa aaatcagatg cgcgacatgc tggagaacgc tgaaaaagaa aaccgcagtc
     4501 ttaacgatgc tgagggcgca aaatttgacg aattacgcgc taaagctgaa tccctcgata
     4561 aagacatttc ccgccttgaa gccattgcag acgaagagcg cagcaagcca ggtaaaagca
     4621 gccagaccac tgaccccgca gaactacgta actacattct gaccggtgaa acccgcgcat
     4681 taagtacagg cgttcccgct gatggtggtt ataccgttat ccctgaactg aacaccgaaa
     4741 tcatgcgaat gctggcggat gaatccacca tgcgtcgcat ctgtaccgtg aagaaaatca
     4801 gcagcaacga gtttaagcag cttgtttccg ctggcggtgc gaccgttaac cacggtgaag
     4861 agggtaaggc acgcgaacag accagcaccc cgcagattaa cgaggtgagc attaagctgt
     4921 atccggtcta tgcgtacccg cgcaccacac aggaaatcgt ggatttttcc gatgtggaca
     4981 tcctttcatg gctgacgggt gagattggcg acaccttcac ggaaaccgaa gaaagcgatc
     5041 tggttgtggg cgacggtgac aaaaaagcaa aaggtttttt atccgtaccc cgtgcagaga
     5101 agaacgacaa agagcgtgat tttggtacgt tgcaggtaat taaaccttcc gaatctctgg
     5161 cgtggacatc tgcggacccg ctgatcgacc tgaaatttgc attacgtaaa aaataccgca
     5221 aaaacgcggt ctgagtggtt aactccacga cggcggcaaa acttcagaag gtgaagaacg
     5281 cgaacggtga ttatatctgg cgcgaccgtt tacaggcggg tgatcctgat acgttgctgg
     5341 gccttccggt cgaatatctg gagtttatgc ctgataacgt tattgccctg ggtgacttca
     5401 aacgcggtta ctacattgtt gatcacgaaa caggtgttcg caccagaccg gacaacctga
     5461 cagaaccagg attcatcaaa attttcacgc aaaaatattt aggcggtggc gtggtggatt
     5521 cgaacgcgat caagattctg gcactgccgg aagacgacga ttaacagcat acagaagggg
     5581 ctaaaaagcc cctttagtgt tttatgggtg aaaaaattat gaagagtatg gaaatccggt
     5641 catcggaaat caccaccagc ggagccggta cgctgacggg ctacgttgtt cgctgggata
     5701 agctttcaga actgttatgg ggtgagtttt acgaaaaatt ccagcggggg gcgtttactg
     5761 aatggcttgc ggcgggtaat gacgttcgcg gcctgtatga gcatgaccac agcatgttac
     5821 tggggcgcac ccgttccggc acgctgaaac tggaagagga cgaaacaggg ttacgctttg
     5881 aactgacccc accggatacc agtacagggc gcgacgttat cgaactggtt aagcgtgggg
     5941 acatctcggg gatgagcttt ggctttcgct cccgtaagga tgtatgggat accacaacag
     6001 atccatgcgt gcgcaccgtg ctggtggcgg aactgtacga aattaccgtt acatcggtac
     6061 cggcttaccc cgattccggc gtggagctgg cccgccgttc cctgtatgag cagcaccccg
     6121 aaaaaatgcc gcgtgcggat aatcgccgct ggtgggcgga tttagcgggg gtgtgatatg
     6181 tggcctttca gacgaaaaaa agagcagcgc agcatgacgc ttgatgaatt tatggcgctg
     6241 gccggcacat cgaacacggg ggcgggtgag tacgtatcat ctgggacagc ggaatcactc
     6301 cccgccgtca tgaacgccgt cacggtcatc tctgacgcgg tggctaccat gccgtgttac
     6361 ctgtacctgg tacgcaatga gaaggggaag gaggcccgcg aatggcttga ttctcatccg
     6421 gtcgatcaca tcctcaacga gcgcccgaac gcgtggcaga caccctacca gtttaagcga
     6481 atgatgatcc gctactgcct gttaagtggt aatgcttatg cggttattca gtgggggcgt
     6541 gatggttttc cggcggcttt acatccttac ccgccgcagt cggtgaacgt ggagcagaca
     6601 ggtgatcata actggcgcta ctgcatcact gacgcctaca ccggaaacat ccgcaactat
     6661 ttaccgtggg aggttctcca ccttcgttac tccacggatg acggttttat ggggcgctca
     6721 cctgtaacca tctgccgcga atcgctgggg cttgggctgg cccaacaacg ccacggcgcg
     6781 agcgtgatgc gtgatggcat gatggcggca ggggttatca cgtcaggcga atggctggac
     6841 ggcgtgaagg gcaaacaggc attagccgca ctggaacgct acaaaggggc cagaaacgcc
     6901 ggaaaaacgc ccatccttga aggggggatg agctatcagc agctgggcat gagtaatcag
     6961 gatgccgaat ggctggcctc ccgtcgcttc accattgaag acatcgcccg aatgttcaac
     7021 gtctcgccga tttttttgca ggaatacagc aacagcacct acagcaactt cagcgaggca
     7081 agccgcgcat ttctcaccat gacgatgcgc ccgtggctgg cgaactttga gcagcagata
     7141 aaaaacgcct tgctggtggc ctcgcctgta cctggtatcc ggtatcaggt ggagtttgac
     7201 agcgcggacc tgttacgggc cacacctggc gaacgctttg ccacctatga gcgcggcata
     7261 aaatccgacg ttatgtgtcc gaacgaagcc cgcgaacgtg aagggctgtc accgcgtgat
     7321 ggtggtgatg agttcagcca ggcatggaaa caggaagtaa aaatcagcga gggagaaaaa
     7381 ccggaatgaa catagggcga ctgcgtgaca ggataacgat tcagaccctg aaacagacca
     7441 gggatataac cggcgaaata ctcgaaacgt gggaggacgg tcacacactc tgggcaagcg
     7501 tgaacatggt cagcagcaag gaggccattt catcgggcgc agagctggcg attggtaccg
     7561 taaggatctg gatacggtac cggaaggaca tcaacgccac cagccggata aaggtcagta
     7621 cggggccgct ggcggggcgt gtactgaata tcatcgggca gccgctgccg gatgccgcca
     7681 ggacacgcct tgaaattctt tgtcgtgagg gcgcggaaaa atgacagaag aacttatcac
     7741 cctggaagaa gtgaaactcc attgccgcat cgatggcgac gatgaagacc agttaatcag
     7801 cggatacatt gccgcatcgc ttgaggcgtg ccagatacac ataggcaggc gctttgatga
     7861 cgggctggcg ttcacgccag ccataaagat tggctgcatg atgtttatcg ctcacctgta
     7921 tgaaaatcgc cagattgtcg cggataacgc aaaaacacgc gtacccatga cgataggcgc
     7981 actctggacg gcttaccgtg atgtgggggg gtactgatgc cgtggcaacc attaagacga
     8041 tgcacagaac caggctgtaa caggcgcgtt aaatccggca agtgtgagga gcacaggcgg
     8101 gcggcatggc gtgcagagga tgccagacga ggacaccgcc gcgcgcgtgg atattccaga
     8161 cagtgggaca aataccgcgc catgtatctg agtaaaaacc cgttatgcgt gcgatgcctt
     8221 gagaagggga tatatacgcc cgccgtggtg gtggatcaca ttatcccgat taatggcggt
     8281 gatgatgttc tcttctggcc cgaatggaac catcaaccat tgtgccaggc gtgtcataac
     8341 cagaaaacga aatggcttga tccggcaaca aaaagcaagc gtgccgcagg tggatttcgt
     8401 gaagaggaag aacgggctgc taaccgcaat aactggatgt atgacgctga tgaatgagcg
     8461 ggaacaaaac cgccttatcc gtggacttat aaggcagcgt gacgcatgga agacacagga
     8521 aacagggcat aaagataaag cgtcaggacg cgcagaacgc atcacagcga agcgattaac
     8581 cgaccgtgac cgcgaggtta tggaatgttt ccgcaatcgc tgatgaggct gtatgacggg
     8641 gtggggggcg ttttcaggac aaacccgacc ccgcctacct ggatgatatc gccgtgaagc
     8701 agtggcggga aaaatcgcgg cagcttgcgg aacggggaga cctgaccccc gccgactgga
     8761 gcaatctgga actgtattgc gtcaactact ccatttaccg gaaagccgtt gcagaccttg
     8821 cggcgcgcgg gttcagcatt gttaacagtc agggcggcga gagcagaaac cccgcattaa
     8881 gcgcaaaatc cgacgctgaa agagtgatga taaaaatggc ctccttgctc ggttttgacc
     8941 cgataagccg ccgtaaaaat ccaccggaaa cagaagaaga ggacgagctt gaccgcctgg
     9001 aataagtacg cagaagacgt aaaaacgggc aaaattccgg cctgtaaacg gttaaaacag
     9061 gctgttaaac ggtacttttc ggaccttaaa agcccccttt acacgttcga tcgtgaggtt
     9121 gtggagcggt ttattgcctt ttccagggtg tgcccgcacg taaaaggacc gatgcgtggc
     9181 agacccattg agctggagcc gtggcagcag ttcgcctttg cgtgcatcct cggctttaag
     9241 gttaaggcca ccggacggcg caaatacacg agcgccttta tcgaagtgcc gcgcaaaaat
     9301 gcgaaatcca cgaccgccgc gattctggct aactggtttc tgattatgga gaacggtcag
     9361 caggatatct acaccgcagc ggtgagccgt gaccaggcgc ggatcgtgtt tgataatgcg
     9421 cgtcagatgt gccttttatc ccgaccgtta cgcaggcggg tgaatattca ggcgcataag
     9481 gtgatacacc cgaaaagcaa cagcctgtta aagccgctgg cagcaaaagc ggcaaccatc
     9541 gaggggacaa accccagtct ttccatcgtg gacgaatacc acctacaccc cgataacggc
     9601 gtttattccg cgcttgagct ggggatgggc gcacgtcccg aagggctgtt atttgccatt
     9661 accacatcgg gcagtaacgt cgtttcagcc tgtaaacagc actatgatta ttgctgccag
     9721 atcctggacg gcgaagaggt taacgattca atttttgtac tgatttacga actggacgac
     9781 gaaaacgagg ttgatgatcc ggcgatgtgg ataaaggcga accctaatat tgatgtttcc
     9841 gtggatcgtg aaaaactggc ctcaaccatc cagaaagcgc ggggtattcc gtcgcagtgg
     9901 gtggagatga tgacaaagcg atttaatatc tggtgtcagg ggactacccc gtggatgggt
     9961 aatggtgcat gggctgagtg tgccggaacg ttcacggagg aagatttaca cggtcaggag
    10021 tgttacgcgg ggctggactt atcatcaacc agcgatattt ccagcgtatg ctatgccttt
    10081 ccggtcggta aaaccattat gctgatttcc cgtcattatc tgccggagtt ccagctacag
    10141 aaccccgcca ataaaaaccg cgctgtctat cgccagtggg ctaaagcggg ctggatacgc
    10201 acaacgccag gtgactgcat tgattacgac cggatcagag atgacatcat gcaggatgcg
    10261 gagaaattta atatcaggct ggtgggcttt gatacgtgga acgccacgca tctcaggacg
    10321 caattacagg gggctggttt tgaggtggag ccgttcccgc aaacctacct cagattcagt
    10381 ccggcggcga aatcgttcga agtttttgtt aaccgcaggg tgattgtgca tcgtggcgat
    10441 ccggtgttgt cctggtcgat gagtaacgtc gtgatgcaga gtgacgcgaa cgccaatatc
    10501 aagccgaaca agaaaaaatc accgaacaag atagacccga gcgtagcggc gctgatggcg
    10561 tttggtacat tccaggcaga gcatgaggat tttgcttttg atataagcga cagccaccgc
    10621 cagaaactgg aagaatttag cggggtgtaa tgaggtcagc agcatgacag aagccgaaat
    10681 actgcgatta atccgccgcg tctctggaat cagccagcag gctgacgaac agaccacgca
    10741 gccggacagc gtgacagccg aaaattatgc gcgtgttgtt gctgaggtga tgcgccgtga
    10801 tggtatccag cttaatgatg cggatatgcg cgacatacgg atccgcgttc tggaaatgct
    10861 ggcctacaat cgccgcgttg aactgtatcg ggagaaggag aaaatcacgt accactggaa
    10921 gaagccggag cggttacggc ggtaactcac tgagattaaa gcaaagcgca aaattgcgct
    10981 ggctacatcc ctcacatctg aggactgtta accagcaggc cggaatatat tcagaaacta
    11041 cgaaattttc gcagtttaat tactcgtcag cttaatcgct aacccgctct gatattttcg
    11101 gaaacctcaa tttgaggaag tcggcgaggt aactgtttga tattcccgat agcgcaaaga
    11161 tgcgcggtcg ggcagaatga ccaccacctc aatcgtgacg gagagtgaca accagtgaca
    11221 caccgtgaca aaaagacggt ttaaccgttc attaaaaatc gacgtgattt tgtgctttcc
    11281 ggatgacgtg gccaacgtca ttttttagcg aaaaattctg attaaggtca ctggctgaac
    11341 ggtcataatg accacgcaga ccatcgaggc aaccatagcg atcatcgtta cggttggagc
    11401 ttttccgcca ttggtggaaa aggtgcacat ctggatcatc attacggtga tcaagggtat
    11461 gtaaaaccca ccagcctgat taaaggttaa ccggaaaaaa aatccggtag ccccgttatg
    11521 gggatcccca tatcgacatt aacgcccctc atgaattggt gcgcttcccc ctggaaagat
    11581 tacccgcctg tatatttctt gtgtctattt gttccacgtt gtttcataca gtgcaccgaa
    11641 cggtgtagtt aatggtgtag tcaatttgcg atttttggca ctttttcaat gaaaattgtt
    11701 tgatcgaatc aatgacttga tgtagtggta aaaggacata tttattcatc tttcggcgca
    11761 gaatgctggc gaccaaaaat cacctccatc cgcgcaccgc ccagcatgct ctctccggcg
    11821 acgattttac cctcatattg ctcggtgatt tcgcgggcta ccgccagccc tacaccttgc
    11881 ccagggcgta aagtatcaac ccgttgacca cggtcgaaaa tgacctctcg cttgcttaat
    11941 ggaataccgg ggccatcatc ctcgaccaca atatagagat gctcgtcggt ttgccttgca
    12001 gaaatttcga caaactcgag gcaatattta caggcattat ccagcacgtt gcccatcacc
    12061 tcgacaaaat cgttctgctc accgacaaag ctgatctctg gcgaaatatc gagagagata
    12121 ttgacccctt tgcgttgata cactttgttc agcgctgagg tgagattgtc cagcagtggg
    12181 gcgaccggat gcagctcgcg gctgagcaat gtcccgccgc gcatactggc acgatgcagg
    12241 tagtagccaa tttgctgtga aatgcggctg atttgctcca gcattaccgg ctcagcatca
    12301 ctgacgctca tcttttcact acgcagagaa cgcagcgtac tttgcagcac cgccagtggc
    12361 gttttcagac tatgggtcag gtcggtgagc gtcgtacggt atttgtcgta acgttcgcgt
    12421 tcacttttta acaatcggtt caggtttcgt accagactgg tcagttctcg cgttgtggct
    12481 ggattgagca attcgcggtt atgttcttcc agttcgcgga cttcttttgc cagggcttcg
    12541 atggggcgta aactccacca ggcggcgacc cacagcagcg ggatcactaa cagcagattg
    12601 gctgagagca catagataaa ccagctccag accatatagg aactttttag ctccaccgga
    12661 atggtatcca ccaccacaat ggttaatttt ggcatccgcg atgttgccgg gtagacgttt
    12721 accgccaccg agtgggtcat ctccgcgtcg tcatcatctt cccgcacttc ctgcaactgt
    12781 tgctgtatcg aatgatctcc actcagcaag aggctggtat cgttaacatc cgcttcaatt
    12841 tcatgaaaac cattcgattt cagccagtca ggctggatca ttttcatcag ccagggcacg
    12901 tcacgttgcg cccataaaag ctgcccgttc tcatcataaa ttagcgtcat ggtggggctt
    12961 tgcttgtcga tattttcggg taactcgaca tgcaacttat tgttttccca ctgcgcaagg
    13021 gtatagaaca gattgctctc gccacgtaac agccgaaacg tagttttatc gaaactgacg
    13081 ctataaccga tcagcgcgac cattccgtag gcaagcgaaa gcaccagtac taccgctgcc
    13141 gttgccaaca gaaaacgtac ccgcagcgag agcgggaaaa aaagacgcag taattttttc
    13201 atcagcgcaa ttcgaacagg tagccctggc cgcgaacggt ggtaatgact tcttgcggat
    13261 attgtgcctg aatttttttg cgcagacgtc ccatcagtac atcaatggta tggctttccc
    13321 gcagctccgc atccggatag agttgtagca ttaacgaatc tttgctgacc actttgccat
    13381 tattgcgtat caacgtttcc ataatggtgt attcgaacgc ggtcagtttg atcacttcgt
    13441 cattaataga taattcacgg cgagagagat caacctgaaa cggggggagc gaaatgacct
    13501 gtgaagccag accgctatta cgccgcatta atgcctgcat tcgcgccatc acctcttcaa
    13561 tatgaaacgg tttagtcacg taatcatcag caccggcact taatacttcg actttgtcct
    13621 gccagctttc acgggcggtt aataccagaa tcggcagtga aacatcgttg ctacgccagc
    13681 ggcgaatcag tgacagaccg tcctcgtcag gcaatccgag atcgacaatc gcaatatccg
    13741 gtaaatgttc attgagataa taatcggctt ctttggcatc ttctgcgtca tcgacctgat
    13801 gaccagcatc ctgaatctga actttaaggt ggtgacgtaa caacgcattg tcttcaacaa
    13861 ccagtacgcg catttttatt tctccctgtc ttaattatta aaatagtgta acgcgattat
    13921 gtgtttatgg gggtaaacat taaataaacc agcggggagg ggaggtaaag tgaaaaaaga
    13981 aaaagtggat aatcttaata agcaggccgg acagcatcgc catccggcac tgatttgagg
    14041 cttatttcag ctcatcaacc atcgtgatag ctcgaccaat atagttagcc ggcgtcatcg
    14101 ctttcaggcg ggctttctct tcttctggca acgccagacc atcgataaac tgcttcatgc
    14161 cttcggcgtc aacgcgctta ccgcgagtaa gctctttcag cttctcgtac ggtttttcga
    14221 tgccatagcg acgcataact gtctggattg gttctgccag cacttcccag ttgtgatcca
    14281 gctcatccag cagatggtca cggttcactt ccagtttgct cacgcctttc agggtggatt
    14341 gatacgcaat cagcgcataa ccgataccca cgccgaggtt acgcagcacg gtggagtcgg
    14401 tcaggtcacg ctgccagcgg gaaaccggca gtttgcttgc cagatgctgc aataccgcgt
    14461 tggaaaggcc cagattccct tcggagtttt cgaagtcgat cgggttaact ttatgcggca
    14521 tggtggaaga accaatctca ccagcaatgg ttttctgttt gaagtggtta agggcgatat
    14581 aaccccagac gtcacggtca aagtcgatca gaatggtgtt gaagcgcgca acgcaatcaa
    14641 acagttcggc aatgtagtcg tgcggttcaa tctgggtagt gtacgggttc cattgaatac
    14701 ccagcgaggt gacgaactct tcgctgaact gatgccagtc aacttccggg taagcggcga
    14761 tgtgggcgtt atagttaccg accgcgccgt tgattttgcc gaggatctcc acctggttaa
    14821 gctggcggtg ctggcgctcc atacggtagg cgacgtttgc catctcttta ccgatggttg
    14881 acggcgtggc tggctgaccg tgggtacgag acagcagcgg gatatcgcgg tactgaacgg
    14941 cgagatcttt aatgccatca atcagttgac gccagtacgg caggatcact tcatcacgcg
    15001 cggttttcag cattaatgcg tgggagaggt tattgatatc ttccgaagta caggcaaagt
    15061 ggatgaattc agaaaccgcg tgcagttccg ggatctccgc cactttttct ttcaggaaat
    15121 actcaaccgc tttaacgtcg tggttagtgg tacgctcgat ggttttgatg cgtgcggcat
    15181 cttcttcgct gaaattggcg acaattgcat caaggtaacc gattgcgtcg gcagcaaaag
    15241 caggaacttc cttgatcgct gcgtgcgcgg ccagtttttg cagccaacgt acttcaactt
    15301 gtacacggaa tttcagcaaa ccatattcgc tgaaaatccc gcgcagcgcg ctgactttat
    15361 cgccgtagcg tccatcgaca ggggaaacgg cggtcagtga ggataattcc atagatcaca
    15421 actccggggt taaatgagca agaatttgtt ttgcctgagt ggtcaggcga ttacgagaaa
    15481 acatcagttg tagacgtccg ccgccgacct ggtgccagag cacggcggcg cgaatgcctg
    15541 ccagcagggt tgcgcgaact ttcgcctgca cttgtgggct ttgcagtaca gcaggggaac
    15601 cggtgacctg aatgcgcggg ccaagcgggc tgatcacatc aacatagata gcagccatcg
    15661 cgctcatcag cgtttcggac tgtaaatcga agtgttcgag ctggcgttgc aggccgttga
    15721 tccggttgcc cagagtgtcg agcgcgcctt tcgctgagga gagtttgcgc tcaagcacca
    15781 tcaagctgag tgtgtagcgg gttaattcgg cgtttaagcc ctggcaactg ctggcattga
    15841 gcacgccgag cagggtttcc agcccgacgc gcaggttggc ttcgctaccg ccaaaaaccg
    15901 ccagcgtcga gctggggttc atatcaataa tactgttgag tgagacgtgt agcgcatcgg
    15961 catcacaatg cccctggtga gcgagttgtt gcaccaggcg tgccgactga caaataccgg
    16021 ccagggcgag ggtgatgtca tagtaattct ttgccacgtt cactgcttcc ttgttttaag
    16081 taaagataat aatcagaccg gcagcggcag acgctgctca ataataccgc caccgaggca
    16141 cacttcaccg ttatagaaga cggcagactg gcccggcgtc acggcggcaa ccggctcatc
    16201 gaaaatcact ttaatgcgat cgtcgtccag cgccttgacg gtgcacggaa tgtcggtctg
    16261 gcgatagcgg gtttttaccg tgcaacgcat agtgccggtg aatggttcgc gatcgaccca
    16321 gtgcagctgc tgggcaatca acccgacaga catcagccgc gggtgttcat ggccctgagc
    16381 gacaaccaga atgttgtttt cgacgtcttt gtccaccaca taccacggtt cttcggtacc
    16441 ttctttggtg ccaccgatac ccagaccttt acgctgaccg agagtgtgat acatcagccc
    16501 ctggtgctca ccaatttcat cgccatcgac ggtaatgatt ttgcccggtt gcgccgggag
    16561 ataacggccc aggaactcgc ggaatttacg ttcgccaatg aagcaaatgc cggtagagtc
    16621 ttttttcttc gcggtgacca gaccaagatc ttcagcaatc ttacgcacct gcggtttttc
    16681 cagttcgcct accgggaaca ggctttgcgc aatctgctca tggctgagcg tataaaggaa
    16741 gtagctctgg tctttattgc tgtccagacc acgcagcagg cggctcttgc catcgacatc
    16801 ggcccgacgt acgtaatgac cggtagcgat ataatcggca cctaaatctt cggcggcaaa
    16861 ttcgaggaag gctttaaatt tgatctcctt gttgcacaga atatccggat tcggcgtgcg
    16921 accggcttta tattcggcaa ggaacagttc gaagacgttg tcccagtact cggcagcaaa
    16981 gttaacggtg tgcagttcaa tgccgagctt gtcgcagacc gcctgggcat cagccagatc
    17041 cgctgctgct gtgcaatatt cctcaccgtc gtcttcttcc cagttcttca taaacaggcc
    17101 ttcgacctga tatccctgtt gttgcaacag ccaggcagaa acggaggaat cgacaccgcc
    17161 ggacatgccg acgattactt tttttgcggt ttcagacatt ggatcactca cgacattgaa
    17221 cttcaaggcg gcgtattcta tcacgcagcc ttttagatga caccctttgt aaaaggccag
    17281 ttaaaatcgc caatcatctc cagcggataa cgttgcccgc tttgataaca acgaatactt
    17341 tccgccacca gcggcgaacg aagatttgac gcctgtaaaa tttcttcggc gctgacccaa
    17401 cggcagcagt cgatatcgct gtcatgaggc tgggtcgggc atatttgctc aagctcaatg
    17461 gcaaagagga aacgcaaaaa cggcgtttta tctggcgcaa tccactgatg catacgaata
    17521 aagtgttgcg gctgcgcgct gatgccggtt tcttcccaca gttcacgggc ggcggcttcc
    17581 actaaggttt catcggcttc cagatgcccg gcaggttggt tccataacgc tttaccatta
    17641 atcgtctctt caacgactaa aaatttgcct tctgcgtgca ccacgcaagc aacggtaacg
    17701 tgcggtttaa acattttgca tccttaatct gtcacttctc gccattcgcc attggcaaga
    17761 ttatccaaag agtaatctcc catcgcatag cgaatcagtc gcagcgtggg gaaaccaaca
    17821 tgggcggtca tgcggcgcac ctggcgatta cgtccttcat ataaggtgat cttcagccag
    17881 ctggtgggaa tacttttgcg ttcacgaatt ggtggattcc gcggccataa ccacgcgggt
    17941 tcgtcaacca gctccgcgcc tgcgggcagg gtagggccat catttaaggt tacgccattg
    18001 cgcaaggctt caagtgcgtc ttgtgtggga ataccttcca cctgcacata atagattttt
    18061 ccggtgcgtt tacccggctg ggttaaacgc gcctgcaacg cgccgttatt ggtcagcacc
    18121 agcaaccctt cgctatcgcg gtcaaggcga cctgctgcat aaacgccctg aaccgggatg
    18181 aattctttta atgttttgcg tccggtttca tcggtgaact gcggaagaac atcgtagggt
    18241 ttattgaaga ggatcacacg cgtgggctgg ttttcatgtt tacgcctggt agaacgttgc
    18301 gagctgaatc gcttaacctg gtgatttcta aaagaagttt tttgcatggt attttcagag
    18361 attatgaatt gccgcattat agcctaataa cgcgcatctt tcatgacggc aaacaatagg
    18421 gtagtattga caagtcaatt acaaatcatt aacaaaaaat tgctctaaag catccgtatc
    18481 gcaggacgca aacgcatatg caacgtggtg gcagacgagc aaaccagtag cgctcgaagg
    18541 agaggtgaat ggaaagtaaa gtagttgttc cggcacaagg caagaagatc accctgcaaa
    18601 acggcaaact caacgttcct gaaaatccga ttatccctta cattgaaggt gatggaatcg
    18661 gtgtagatgt aaccccagcc atgctgaaag tggtcgacgc tgcagtcgag aaagcctata
    18721 aaggcgagcg taaaatctcc tggatggaaa tttacaccgg tgaaaaatcc acacaggttt
    18781 atggtcagga tgtctggctg cctgctgaaa cccttgatct gattcgtgaa tatcgcgttg
    18841 ccattaaagg cccgctgacc actccggttg gtggcggtat tcgttctctc aacgttgccc
    18901 tgcgtcagga actggatctc tacatctgcc tgcgtccggt acgttactat cagggcactc
    18961 caagcccggt taaacaccct gaactgaccg atatggttat cttccgtgaa aactcggaag
    19021 acatttatgc gggtatcgaa tggaaagctg actctgccga cgccgagaaa gtgattaaat
    19081 tcctgcgtga agagatgggc gtgaagaaaa ttcgcttccc ggaacattgc ggtatcggta
    19141 ttaagccgtg ttctgaagaa ggcaccaaac gtctggttcg tgcagcgatc gaatacgcaa
    19201 ttgctaacga tcgtgactct gtgaccctgg tgcacaaagg caacatcatg aagttcaccg
    19261 aaggcgcgtt taaagactgg ggctaccagc tggcgcgtga agaatttggc ggtgaactga
    19321 tcgacggcgg cccgtggctg aaagttaaaa acccgaatac cggcaaagag atcgtcatta
    19381 aagacgtgat tgctgatgca ttcctgcaac agatcctgct gcgtccggct gaatatgatg
    19441 ttatcgcctg tatgaacctg aacggtgact acatttctga cgctctggca gcgcaggttg
    19501 gcggtatcgg tatcgcccct ggagcaaaca tcggtgacga atgcgccctg tttgaagcca
    19561 cccacggtac tgcgccgaaa tacgccggtc aggacaaagt aaaccctggc tctattattc
    19621 tctccgctga gatgatgtta cgccatatgg gctggactga agccgcagac ctgattgtta
    19681 aaggtatgga aggcgcgatc aacgccaaga ccgtaaccta tgacttcgag cgtctgatgg
    19741 aaggcgctaa actgctgaaa tgttcagagt ttggtgatgc gatcatcaag aatatgtaat
    19801 cactacatgt gttaaatatt taaacgggcg tataacacgc ccgttgtttt atttatgtgg
    19861 atattattaa tagcatatcg agcata
//
LOCUS       DAJTSI010000073        19698 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000073 DAJTSI010000000
VERSION     DAJTSI010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.073"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(151..1584)
                     /gene="hldE"
                     /locus_tag="OO327_002324"
     CDS             complement(151..1584)
                     /gene="hldE"
                     /locus_tag="OO327_002324"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR6018847.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="OO327_002325"
     CDS             complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="OO327_002325"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR6018848.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="OO327_002326"
     CDS             complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="OO327_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR6018849.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6038..6658
                     /locus_tag="OO327_002327"
     CDS             6038..6658
                     /locus_tag="OO327_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR6018850.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6722..7960
                     /gene="cca"
                     /locus_tag="OO327_002328"
     CDS             6722..7960
                     /gene="cca"
                     /locus_tag="OO327_002328"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR6018851.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     IEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="OO327_002329"
     CDS             complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="OO327_002329"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6018852.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9035..9403)
                     /gene="folB"
                     /locus_tag="OO327_002330"
     CDS             complement(9035..9403)
                     /gene="folB"
                     /locus_tag="OO327_002330"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR6018853.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9508..10125
                     /gene="plsY"
                     /locus_tag="OO327_002331"
     CDS             9508..10125
                     /gene="plsY"
                     /locus_tag="OO327_002331"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR6018854.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="OO327_002332"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="OO327_002332"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            11276..12119
                     /gene="ttdA"
                     /locus_tag="OO327_002333"
                     /pseudo
     CDS             11276..12119
                     /gene="ttdA"
                     /locus_tag="OO327_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            12116..12721
                     /gene="ttdB"
                     /locus_tag="OO327_002334"
     CDS             12116..12721
                     /gene="ttdB"
                     /locus_tag="OO327_002334"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR6018855.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            12769..14232
                     /gene="ttdT"
                     /locus_tag="OO327_002335"
     CDS             12769..14232
                     /gene="ttdT"
                     /locus_tag="OO327_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR6018856.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="OO327_002336"
     CDS             complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="OO327_002336"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR6018857.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15526..15741
                     /gene="rpsU"
                     /locus_tag="OO327_002337"
     CDS             15526..15741
                     /gene="rpsU"
                     /locus_tag="OO327_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR6018858.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            15852..17597
                     /gene="dnaG"
                     /locus_tag="OO327_002338"
     CDS             15852..17597
                     /gene="dnaG"
                     /locus_tag="OO327_002338"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6018859.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            17792..19633
                     /gene="rpoD"
                     /locus_tag="OO327_002339"
     CDS             17792..19633
                     /gene="rpoD"
                     /locus_tag="OO327_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR6018860.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         4683 a         5506 c         5031 g         4478 t
ORIGIN      
        1 gttgttactg ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc
       61 cttacccttg ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct
      121 acgaagcgag atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt
      181 gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt
      241 ggcccagact tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag
      301 taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac
      361 ccagtcgacc gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg
      421 gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg
      481 gtcacccagt ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca ggatgtcaaa
      541 gacgccattg gtcatcacca ctttttcacc gcgtttacgc gctgccgcta cggccagctt
      601 cagttcctct tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc
      661 cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc
      721 attggcaaag aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac
      781 gccaatcacc gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat
      841 atgcagcggc gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa
      901 cagagccgag agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc
      961 ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc
     1021 gccgcggtag cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt
     1081 acgcgccagt tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag
     1141 caccagcgcg ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc
     1201 aacaccttcg aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac
     1261 ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac
     1321 gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag
     1381 gcgtgcatta gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg
     1441 ttcttcgatg gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt
     1501 ggggccgtac cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc
     1561 aaactctggc agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat
     1621 aataccatac ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc
     1681 tctgcggtga agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga
     1741 agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata
     1801 atgtcgtttt gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc
     1861 ggtttttcat gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt
     1921 ccgccttcat cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc
     1981 attttctcgc gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc
     2041 ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca
     2101 cgcgcacgca ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca
     2161 aatgcttctg cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc
     2221 acttcataca aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc
     2281 agacgcagat aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt
     2341 gggcaatcat ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc
     2401 agcttgccgt agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc
     2461 ttaccgtagc gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct
     2521 tctgccagcc aggttaagtg atcgctcact ttcattaccg gtagcgtacc ggcgatatcc
     2581 gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgt
     2641 tcttcgtcat cttccggcac gcgcagcaaa tactggcgca gttcatcacg gtaggcatcg
     2701 gtcgccgtcg gctgataaag ggtatttgga tcgagcaatt catccagcaa taatggataa
     2761 cgcgctagct ggctggcaat catcggcgat gcggcacaca gagaaatcaa atgtttaagc
     2821 gccgcaggga attcactgag caattctaaa taggttgtgc gggtaacaat ccccaccagc
     2881 aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc
     2941 agatgtggca tcagatggtc gagcacctga cgtcctcgcg gcccgatagt acgcttatcc
     3001 agctctttgc ggaaatcagc aatcatcgtt aggacctgtt tgcgatcatc ctcgctaaga
     3061 tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac
     3121 tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac
     3181 acccggcgca cattggtcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca
     3241 aagtccatcg cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtttgggtt
     3301 tgttcgtcgt taatgctttg cagcaggttt tccagacgcc gcaggaacag atatgccact
     3361 cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg cggcaatggc gctgagcgtt
     3421 ggcagtaaag agcgcgactg cagcaacggt tcgcgcccac cgcgaatgag ctggaacacc
     3481 tgaacgataa attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa
     3541 ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg
     3601 ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca
     3661 tagacgcctt cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg
     3721 cgtccctgct cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt
     3781 tcgccaaacg gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga
     3841 tccagcactt taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc
     3901 cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag
     3961 gagaaattca gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct
     4021 tcgccctgcg cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca
     4081 cgcgccgcaa caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca
     4141 gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc
     4201 cgcagctcgc gcattaaccc ggcttcgtcg ctcacattac tgagcgcctc ctgcaaccac
     4261 acgctgtaat gttgccattc gtcagcctgt ggcggttggc tttccagttc cgtcagccac
     4321 tctggatgcg caatcacgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc
     4381 tgtgcactaa gtgattcctc ggctaaaagc tctggcagcc gctcaacaac ggtctgccag
     4441 tactgctgta acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg
     4501 ttttccgctg tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat
     4561 ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc
     4621 ttgcgcgacg acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcat
     4681 caggcgtggc aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag
     4741 ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctaatttt
     4801 gccctgcgct ttggcatcta aaaacggctg ccatgctttg cttaccagcc attcggtcaa
     4861 cgccagcttc gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga
     4921 agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag tgagtactcg ctttacgcgg
     4981 cacaatgcca ccgaacagca tcagtgtatg acggactagg ctaatggctg ccagcacctg
     5041 ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa
     5101 caccagctcg agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa
     5161 aatggtggtc ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc
     5221 cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt
     5281 cagcaccgcg cgcgtgtcgc cgctaagcag ctccagttcc agctcgcaga taggctcagc
     5341 aaattcaccc gctttcactt ccccctggtc gagagcgatt tcaattcggc tgccatcgat
     5401 ctctactagc catttttcgc gataaaaatc ggtgctaaac agcgactgca cgcgggaagc
     5461 gagatcggca ggcaattcgc cgttcggcca gatttccgtc ggtaactgcg ccaggtcgag
     5521 cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct
     5581 tcctgcaact ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat
     5641 atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg
     5701 gtcatggtgc tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg
     5761 attaacaata aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca
     5821 cataagtgac gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg
     5881 cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaaga
     5941 cctgacacag aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta
     6001 ctatcgttcc actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg
     6061 attaacttta ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt
     6121 ttctgacgaa ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg
     6181 cacggttaac gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc
     6241 ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga
     6301 gccaagcctg cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa
     6361 actcaccaat atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc
     6421 gcagagcgac agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct
     6481 gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac
     6541 catcatcatg caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg
     6601 tctggtactg ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat
     6661 cgccttcttc gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg
     6721 cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca ttgttaggac tatcggtcaa
     6781 agacagagat tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca
     6841 gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct
     6901 ggcacgtacc gaacggaaat ccggttccgg ttacaccggt tttacttgct ataccgcacc
     6961 ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca
     7021 ggacgataac ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct
     7081 gttgcgccat gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg
     7141 ttttgctgcg cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat
     7201 gcgcgagatg acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga
     7261 gacggaaagc gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg
     7321 cggcgcactt cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcacctgc
     7381 caggtggcat ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc
     7441 gatgctgagt ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg
     7501 actgacgccg ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt
     7561 agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact
     7621 ggtggctgag tttcacgatc tcatccgcac cttcccaatg ctgaacccaa aaaccatcgt
     7681 caaattattt gattccatcg atgcctggcg taaaccgcag cgtatcgagc aactggcgct
     7741 gaccagcgag gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg
     7801 ccgttggttg cgcgaaggct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga
     7861 agcgggattt aaaggcgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt
     7921 agccagctgg aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttagc
     7981 acgaaccgtc ccctcgcccc ttcggggaga gggttagggt gaggggaaca gaccagcacc
     8041 ggtgcgaaca atcaccccca ccccggccct ctccctacag agcgagggag aaaacccggc
     8101 ctgctcccga accttttctc aatcaaaaga acacgacata caccgcagcc gccacaataa
     8161 agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg aaggttttaa
     8221 tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc gggatatcgc
     8281 cggttgtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg
     8341 gtaccgccag caggaacgag aactcggaag cagcgtaacg gctcacgccc atcagcatcc
     8401 cacctgaaat ggtcgccccg gaacgggaga aacccggcca cagcgccaga cactggaaac
     8461 agccaatcat aaacgcctga cgataggtca tatcatcaag acccggcgca cgcggctctt
     8521 tcggcttcag gcattcggcg gcaatcagca acaaaccgcc aacgaccagc gcatacatca
     8581 catttatcgg gttaaacaat gacttaatcg tgtcgtggaa taatagcccc agtaccaccg
     8641 ccggaatcat ccccagcaaa atgtggatca gcgttaaacg acctttgctt tcaccttcgt
     8701 gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa cagacgccgc cagaacatca
     8761 ctactaccgc cagaattgat cctaactgga tcacaacttc aaaggttttc gccgtgtcgc
     8821 cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat accggcagaa
     8881 attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcgag tgcatatcgc
     8941 tcatcaataa acccctaaat tattaaaatg taccgcttgt ccgaactact gcagtatgac
     9001 caggttataa ccgtttggtt taacagctgt aaaattaatt attttctttc agattattgc
     9061 cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc ttgctgagtt
     9121 tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc tcttcagcca
     9181 cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt tctgcaatgt
     9241 cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg ttatcccatg
     9301 ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg taaacaccaa
     9361 tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc tgcctgcttt
     9421 ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat tcatcgttga
     9481 acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat gatcctcatc
     9541 gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg tgggctgccc
     9601 gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg tatcggtggc
     9661 aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt gcctgtctgg
     9721 ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat cgccgcctgt
     9781 cttggacaca tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt tgctaccgct
     9841 tttggagcaa tcgcaccaat tagctgggat ctcaccggag taatggcggg aacctggtta
     9901 ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc actgattgct
     9961 ccgttttatg tctggtggtt taagccacaa ttcaccttcc cggtttcgat gctctcttgc
    10021 ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca ggagacaaaa
    10081 atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct ggtggatcta
    10141 catgacctga tagccttcat cgggcttgcc cagccgttgc tggcaccatg ccgccagaaa
    10201 ttccgcgcag acacgaattt catgctgcga tagagcagct cccggtaaac agcccagata
    10261 ttggcgcttt gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc
    10321 aacacatccc actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact
    10381 atctcgccgc tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg
    10441 ttccccaact cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga
    10501 cgacttaatt cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg
    10561 cataatattc ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca
    10621 ttaatacgaa tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat
    10681 tcaaaatgca cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata
    10741 tggctgcgcc caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg
    10801 atttgcgtga cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg
    10861 taacaacgtt ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt
    10921 gttgccagcg tattttcaag gatttggatc cgcttggtga caaatgcagg cgtttgccca
    10981 agcgtcgccg ctgcagcact aaaactcccg ctgtggacga tttctaccag tacctgcaag
    11041 tctttggcta aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag
    11101 tgtatttcgc aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg
    11161 ttgattcacg aactgttaat tacatattaa cggtggcaaa aaatataaaa ccacattttt
    11221 agtgttagtt tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat
    11281 gagcgaaagt aataagcaac aggcagtgaa taagttgaca gagattgtcg ctaactttac
    11341 cgccatgatt tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga
    11401 tgccgaaacg tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa
    11461 agcgattgac ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa
    11521 agtcggttcc cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga
    11581 agaggcaacc gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa
    11641 caccggcaaa aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa
    11701 tgacgatgcg gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc
    11761 gaaagtgtta atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat
    11821 ctccaccctc gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc
    11881 ggtggaaacc gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca
    11941 tcccaatcca aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg
    12001 gattggtcca caagggctga ccggcaacag ttcagtgatg ggcgcatcgt cgcggcacgc
    12061 tgctggttca tgccgatctc acctttgaaa atctgtctca cacccggagc gcgttatgaa
    12121 aaagatcctg acaaccccga tcaaagctga agatctgcaa gatattcgcg tcggcgatgt
    12181 gatctacctg accggtacgc tggtgacctg ccgcgacgtt tgtcaccgcc gtttgatcga
    12241 actgaaacgt ccgatccctt acgatctcaa cggcaaagcg attttccacg ctggccccat
    12301 cgtgcgcaaa aacggcgaca aatgggagat ggtctccgtc ggcccgacaa ccagtatgcg
    12361 tatggaaagt tttgaacgtg aatttattga gcagaccggc gtaaaactgg tggttggcaa
    12421 aggtggtatg gggccgctga ccgaagaagg ctgccagaaa ttcaaggcgt tacatgtgat
    12481 tttcccagca ggctgcgcgg tgctggcggc aacccaggta gaagagattg aagaagtgca
    12541 ctggacagaa ctcgggatgc cggagtcact gtgggtctgc cgggtcaaag agttcggccc
    12601 gctgattgtc tctattgata cccacggcaa caacctgata gccgaaaaca aaaagctgtt
    12661 cgccgaacgc cgcgatccca tcgtggaaga gatctgcgag cacgtccatt acatcaaata
    12721 accctcccgg agaggctcac ccctctcctt tttcgcaggc ataacacgat gaaaccttcc
    12781 actgaatggt ggcgatatct tgcgccgctg gcggtcatcg ccattattgc tctacttccg
    12841 gttcccgcag ggctggagaa ccatacctgg ctctactttg ccgtttttac tggcgtgatc
    12901 gttggactga tcctcgaacc cgtgccgggt gccgtggtgg cgatggtggg tatctccatc
    12961 atcgccatac tctctccctg gctgctgttc agccccgaac agctcgccca gccaggcttt
    13021 aagttcaccg ccaaatccct ctcctgggca gtttctggtt tttctaattc ggttatctgg
    13081 ctcattttcg ccgcctttat gtttggcaca ggctatgaaa aaaccgggct tggacgccgc
    13141 attgcgctga ttctggtgaa aaagatgggg catcgcacgc tgtttctcgg ctatgcggtg
    13201 atgttctccg agctgatcct ggcacctgta acaccgtcca actcggcgcg tggtgcgggt
    13261 attatctatc ccatcatccg taacctgcca ccgctctatc aatcacaacc aaacgacagc
    13321 agttcgcgca gcattggctc gtacatcatg tggatgggga ttgttgccga ctgcgtgacc
    13381 agcgccattt tcctgacggc gatggcacct aacttgctgt taattggact gatgaaaagc
    13441 gcatctcacg ccacgctgag ttggggcgac tggttcctcg ggatgttgcc gctcagcatt
    13501 ttactggttc tgctggttcc ctggctggct tacgtgctgt acccgccggt actgaagtct
    13561 ggtgatcagg tgccgcgctg ggcagagacg gaactgcagg caatgggccc gctctgttcg
    13621 cgtgaaaaac ggatgctggg gctgatggta ggcgcgctgg tgctgtggat tttcggcggt
    13681 gattatatcg atgccgcgat ggtcggttac agcgtggtgg cgctgatgct gcttctgcgc
    13741 attatcagtt gggacgacat tgtcagtaat aaagcggcat ggaacgtttt cttctggctg
    13801 gcctcgctta tcaccctcgc gaccggactc aacaataccg gttttattag ctggtttggc
    13861 aaactgttag caggcagctt aagtggttat tcgccgacga tggtgatggt gacgttgatt
    13921 gtggtgtttt atctactgcg ctactttttc gccagcgcca cggcgtatac ctgcgccctc
    13981 gcaccgatga tgatcgccgc cgcgctggcg atgccggaaa tcccgctgcc ggtgttctgc
    14041 ctgatggttg gcgcggcaat tggtctgggg agcattctta caccatacgc caccggaccc
    14101 agtccgattt actacggtag tggttatctg ccaacggcgg attactggcg actgggggcg
    14161 atttttgggc tgatattcct cgtgttgctg gtgattaccg gcttactgtg gatgcccgtg
    14221 gtgttgcttt aagtgaggac ggtgccggaa attgctctcc ggcacccaat agttttacgc
    14281 agccggtaac tccgccagcg gccagcgcgg acgcacgcta acgccgagat ccgccgtcgc
    14341 gcctgcttta aaccgcacca ttccggcata ggcgatcatc gcgccgttat cagtacaaaa
    14401 ttccggacgc gcgtagaaca cttcgccgcg gcgttttttc atcatttcag ccagcttcgc
    14461 ccgtaacgtg cggttagcac tcacgccgcc cgccatgacc agtcgcttaa agcccgtctg
    14521 atccaacgct cgcttacact taatcatcaa cgtatcgacc accgcatctt caaaggcgcg
    14581 ggcgatatca gcacgcgtct ggtcgtcggt gccgttgtca cgaatcgtgt tcgccgcaaa
    14641 ggttttcaga ccagaaaagc tgaaatccag ccccggacgg tcggtcatcg gacgcgggaa
    14701 aacaaagcgc ccggcagtac cctgagccgc cattttcgac agtaacggtc cgccaggata
    14761 atccagcccc agcagcttcg cggttttatc aaacgcttca ccggcggcat catcgataga
    14821 ctcgccgagc agctcgtact gaccaatgcc agtcacgctg attaactgcg tatgaccgcc
    14881 ggacaccagc agcgcgacaa acggaaattc cggcgggtta tcttccagca tcggcgctaa
    14941 cagatgccct tccatatggt gtaccgggat tgccggaacg ttccaggcaa acgccagaga
    15001 acgccccacg gttgcgccaa ccagcagtgc gccgactaat ccagggcctg cggtataggc
    15061 cacagcatca atgtcttttg ccgttaaacc agactccttt agcgccgcct ggatcaacgg
    15121 tacggtttta cgcacatgat cgcgggaggc cagttcaggc acgacgccgc cgtagtcagc
    15181 gtgcaatttc acctgactat acaattggtt ggctaacaaa cctttttcat cgtcgtaaat
    15241 ggcgatgccg gtttcatcgc aggaagtttc aatacccagt acacgcatga cttgttttac
    15301 ctcgctttat taccgcgcag tgtaggacca atgcgggttg atgtaaaact ttgttcgccc
    15361 ctggagaaag cctcgtgtat actcctcacc cttataaaag tccctttcaa aaaaggccgc
    15421 ggtgctttac aaagcagcag caattgcagt aaaattccgc accattttga aataagctgg
    15481 cgttgatgcc agcggcaaac cgaattaatc aaaggtgaga ggcacatgcc ggtaattaaa
    15541 gtacgtgaaa acgagccgtt cgacgtagct ctgcgtcgct tcaagcgttc ctgcgaaaaa
    15601 gcaggtgttc tggcggaagt tcgtcgtcgt gagttctatg aaaaaccgac taccgaacgt
    15661 aagcgcgcta aagcttctgc agtgaaacgt cacgcgaaga aactggctcg cgaaaacgca
    15721 cgccgcactc gtctgtacta attccccgag agcgttgctc tccgatcaga ccgagttgta
    15781 gttgtaaggc cgtgcttccg aaaggaatgc gcggcttatt ttcgtttatg aattgctaaa
    15841 aatcggggcc tatggctgga cgaatcccac gcgtattcat taatgatctg ctggcacgca
    15901 ctgacatcgt cgatctgatc gatgcccgtg tgaagctgaa aaagcagggc aagaatttcc
    15961 acgcgtgttg tccattccac aacgagaaaa ccccgtcatt caccgttaac ggtgagaaac
    16021 agttttacca ctgctttgga tgtggcgcgc acggcaacgc gatcgacttc ctgatgaact
    16081 acgacaagct cgagttcgtc gaaacggtcg aagagctggc agcaatgcac aatcttgaag
    16141 tgccatttga agcaggcagc ggccccagcc agatcgagcg ccatcagagg caaacgcttt
    16201 atcagttgat ggacggtctg aatacgtttt accaacaatc tttacaacaa cctgttgcca
    16261 cgtctgcgcg ccagtatctg gaaaaacgcg gattaagcca cgaggttatc gctcgctttg
    16321 cgattggttt tgcgcccccc ggctgggaca acgtcctgaa gcggtttggc ggcaatccag
    16381 aaaatcgcca gtcattgatt gatgcgggga tgttggtcac taacgatcag ggacgcagtt
    16441 acgatcgttt ccgcgaacgg gtgatgttcc ccattcgcga taaacgcggt cgggtgattg
    16501 gttttggcgg gcgcgtgctg ggcaacgata cccccaaata cctgaactcg ccggaaacag
    16561 acattttcca taaaggccgc cagctttacg gtctttatga agcgcagcag gataacgctg
    16621 aacccaatcg tctgcttgtg gtcgaaggct atatggacgt ggtggcgctg gcgcaatacg
    16681 gcattaatta cgccgttgcg tcgttaggta cgtcaaccac cgccgatcac atacaactgt
    16741 tgttccgcgc gaccaacaat gtcatttgct gttatgacgg cgaccgtgca ggccgcgatg
    16801 ccgcatggcg agcgctggaa acggcgctgc cttacatgac agacggccgt cagctacgct
    16861 ttatgttttt gccggatggc gaagaccctg acacgctggt acgaaaagaa ggtaaagaag
    16921 cgtttgaagc gcggatggag caggcgatgc cactctccgc atttctgttt aacagtctga
    16981 tgccgcaagt tgatctgagt acccctgacg ggcgcgcacg tttgagtacg ctggcactgc
    17041 cattgatatc gcaagtgccg ggcgaaacgc tgcgaatata tcttcgtcag gaattaggca
    17101 acaaattagg catacttgat gacagccagc ttgaacgatt aatgccaaaa gcggcagaga
    17161 gcggcgtttc tcgccctgtt ccgcagctaa aacgcacgac catgcgtata cttatagggt
    17221 tgctggtgca aaatccagaa ttagcgacgt tggtcccgcc gcttgagaat ctggatgaaa
    17281 ataagctccc tggacttggc ttattcagag aactggtcaa cacttgtctc tcccagccag
    17341 gtctgaccac cgggcaactt ttagagcact atcgtggtac aaataatgct gccacccttg
    17401 aaaaactgtc gatgtgggac gatatagcag ataagaatat tgctgagcaa accttcaccg
    17461 actcactcaa ccatatgttt gattcgctgc ttgaactgcg ccaggaagag ttaatcgctc
    17521 gtgagcgcac gcatggttta agcaacgaag aacgcctgga gctctggaca ttaaaccagg
    17581 agctggcgaa aaagtgattt aacggcttaa gtgccgaaga gcgatcggga agcccccgac
    17641 agccgcactg agaggcagcg gcaaatatat aagtacgccc tcgtaattat cgttggcggt
    17701 aaacaaccgt tggatttcag cgttaacggc tgaaggacat cgggtcaatc gcccaacacc
    17761 aacctcatga aataagtgtg gataccgtct tatggagcaa aacccgcagt cacagctgaa
    17821 acttcttgtc acccgtggta aggagcaagg ctatctgacc tatgccgagg tcaatgacca
    17881 tctgccggaa gatatcgtcg attcagatca gatcgaagac atcatccaaa tgatcaacga
    17941 catgggcatt caggtgatgg aagaagcacc ggatgccgat gatctgatgc tggctgaaaa
    18001 caccgcggac gaagatgctg ccgaagccgc cgcgcaggtg ctttccagcg tggaatctga
    18061 aatcgggcgc acgactgacc cggtacgcat gtacatgcgt gaaatgggca ccgttgaact
    18121 gttgacccgc gaaggcgaaa ttgacatcgc taagcgtatt gaagacggga tcaaccaggt
    18181 tcaatgctcc gttgctgaat atccggaagc gatcacctat ctgctggaac agtacgatcg
    18241 tgttgaagca gaagaagcgc gtctgtccga tctgatcacc ggctttgttg acccgaacgc
    18301 agaagaagat ctggcaccta ccgccactca cgtcggttct gagctttccc aggaagatct
    18361 ggacgatgac gaagatgaag acgaagaaga tggcgatgac gacagcgccg atgatgacaa
    18421 cagcatcgac ccggaactgg ctcgcgaaaa atttgcggaa ctacgcgctc agtacgttgt
    18481 aacgcgtgac accatcaaag cgaaaggtcg cagtcacgct gccgctcagg aagagatcct
    18541 gaaactgtct gaagtattca aacagttccg cctggtgccg aagcagtttg actacctggt
    18601 caacagcatg cgcgtcatga tggaccgcgt tcgtacgcaa gaacgtctga tcatgaagct
    18661 ctgcgttgag cagtgcaaaa tgccgaagaa aaacttcatt accctgttta ccggcaacga
    18721 aaccagcgat acctggttca acgcggcaat tgcgatgaac aagccgtggt cggaaaaact
    18781 gcacgatgtc tctgaagaag tgcaccgcgc cctgcaaaaa ctgcagcaga ttgaagaaga
    18841 aaccggcctg accatcgagc aggttaaaga tatcaaccgt cgtatgtcca tcggtgaagc
    18901 gaaagcccgc cgtgcgaaga aagagatggt tgaagcgaac ttacgtctgg ttatttctat
    18961 cgccaagaaa tacaccaacc gtggcttgca gttccttgac ctgattcagg aaggtaacat
    19021 cggcctgatg aaagcggttg ataaattcga ataccgccgt ggttacaagt tctccaccta
    19081 cgcaacctgg tggatccgtc aggcgatcac ccgttctatc gcggatcagg cgcgcaccat
    19141 ccgtattccg gtgcatatga ttgagactat caacaaactc aaccgtattt ctcgccagat
    19201 gctgcaagag atgggccgtg aaccgacgcc ggaagaactg gctgaacgta tgctgatgcc
    19261 ggaagacaag atccgcaaag tgctgaagat cgccaaagag ccaatctcca tggaaacgcc
    19321 gatcggtgat gatgaagatt cgcatctggg ggatttcatc gaggatacca ccctcgagct
    19381 gccgctggat tctgcgacca ccgaaagcct gcgtgcggca acgcacgacg tgctggctgg
    19441 cctgaccgcg cgtgaagcaa aagttctgcg tatgcgtttc ggtatcgata tgaacaccga
    19501 ccacacgctg gaagaagtgg gtaaacagtt cgacgttacc cgcgaacgta tccgtcagat
    19561 cgaagcgaag gcgctgcgca aactgcgtca cccgagccgt tctgaagtgt tgcgtagctt
    19621 cctggacgat taatcggtag gccggatcag gcgttacgcc gcacccggca ctagtctctc
    19681 tgcacaaacg ccaccttt
//
LOCUS       DAJTSI010000074        19697 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000074 DAJTSI010000000
VERSION     DAJTSI010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.074"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            206..1303
                     /gene="mltA"
                     /locus_tag="OO327_002340"
     CDS             206..1303
                     /gene="mltA"
                     /locus_tag="OO327_002340"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR6018861.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1541..2347
                     /gene="tcdA"
                     /locus_tag="OO327_002341"
     CDS             1541..2347
                     /gene="tcdA"
                     /locus_tag="OO327_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR6018862.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2398..2841)
                     /gene="csdE"
                     /locus_tag="OO327_002342"
     CDS             complement(2398..2841)
                     /gene="csdE"
                     /locus_tag="OO327_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR6018863.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2841..4046)
                     /gene="csdA"
                     /locus_tag="OO327_002343"
     CDS             complement(2841..4046)
                     /gene="csdA"
                     /locus_tag="OO327_002343"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR6018864.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4238..4465
                     /locus_tag="OO327_002344"
     CDS             4238..4465
                     /locus_tag="OO327_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR6018865.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            4816..5733
                     /gene="gcvA"
                     /locus_tag="OO327_002345"
     CDS             4816..5733
                     /gene="gcvA"
                     /locus_tag="OO327_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR6018866.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5752..6147
                     /locus_tag="OO327_002346"
     CDS             5752..6147
                     /locus_tag="OO327_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR6018867.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6140..7240
                     /gene="rlmM"
                     /locus_tag="OO327_002347"
     CDS             6140..7240
                     /gene="rlmM"
                     /locus_tag="OO327_002347"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR6018868.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7284..8015)
                     /gene="fucR"
                     /locus_tag="OO327_002348"
     CDS             complement(7284..8015)
                     /gene="fucR"
                     /locus_tag="OO327_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR6018869.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(8073..8495)
                     /gene="fucU"
                     /locus_tag="OO327_002349"
     CDS             complement(8073..8495)
                     /gene="fucU"
                     /locus_tag="OO327_002349"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR6018870.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8497..9916)
                     /gene="fucK"
                     /locus_tag="OO327_002350"
                     /pseudo
     CDS             complement(8497..9916)
                     /gene="fucK"
                     /locus_tag="OO327_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            complement(10025..11800)
                     /gene="fucI"
                     /locus_tag="OO327_002351"
     CDS             complement(10025..11800)
                     /gene="fucI"
                     /locus_tag="OO327_002351"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCR6018871.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11833..13149)
                     /gene="fucP"
                     /locus_tag="OO327_002352"
     CDS             complement(11833..13149)
                     /gene="fucP"
                     /locus_tag="OO327_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR6018872.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            13696..14343
                     /gene="fucA"
                     /locus_tag="OO327_002353"
     CDS             13696..14343
                     /gene="fucA"
                     /locus_tag="OO327_002353"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR6018873.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14371..15519
                     /gene="fucO"
                     /locus_tag="OO327_002354"
     CDS             14371..15519
                     /gene="fucO"
                     /locus_tag="OO327_002354"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR6018874.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15572..16327)
                     /gene="xni"
                     /locus_tag="OO327_002355"
     CDS             complement(15572..16327)
                     /gene="xni"
                     /locus_tag="OO327_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR6018875.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(16438..17805)
                     /gene="sdaB"
                     /locus_tag="OO327_002356"
     CDS             complement(16438..17805)
                     /gene="sdaB"
                     /locus_tag="OO327_002356"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR6018876.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17863..19152)
                     /gene="sdaC"
                     /locus_tag="OO327_002357"
     CDS             complement(17863..19152)
                     /gene="sdaC"
                     /locus_tag="OO327_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR6018877.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         4865 a         4821 c         5197 g         4814 t
ORIGIN      
        1 gtggatgcgc actatactcc gctcgcgttt tcatgcaacc ttttttttac cccgatcacg
       61 gatttagcat gattttgaac aaaaacaaac cactaaagcc tattttttgt gcaaaatttg
      121 cgtcagggag aggctgctca tttcattaaa cgcggcggtt tgttatcttc gttgcgcctt
      181 attttttaac ctgaagaaga gaacaatgaa aggacgttgg gtaaagtacc ttcttatggg
      241 cacggttgtg gcaatgcttg ccgcctgctc ttccaaacca accgatcgcg gacagcaata
      301 taaagacggg aaatttaccc agcctttctc tctggtgaac cagccagatg ccgttggcgc
      361 gccgattaac gccggtgatt ttgccgagca aattaaccat atccgtaatt cgtcaccgcg
      421 tctgtatggc aaccagagta atgtttataa cgcggtgcaa gagtggctgc gcgcaggcgg
      481 tgatacccgc aatatgcgcc agttcggcat tgatgcctgg cagatggaag gtgccgacaa
      541 ctatggtaac gtgcagttta caggctatta cacgccggta attcaggcgc gccatacccg
      601 ccagggcgag ttccagtatc ctatttaccg tatgccgcca aaacgtggtc gtctgccgtc
      661 tcgtgcggag atctacgccg gggcattggg cgataaatac atcctcgctt acagcaactc
      721 tctgatggat aacttcatta tggatgtgca gggtagtggg tatatcgact ttggtgatgg
      781 cagtccgctt aactttttca gctatgcagg gaaaaacggt catgcctatc gcagcattgg
      841 taaggtgctg atcgaccgtg gcgaagtgaa aaaagaagat atgtcgatgc aggcgattcg
      901 tcattggggc gaaacacaca gtgaagccga ggttcgcgag ctgctggaac agaacccgtc
      961 tttcgtcttc tttaaaccgc aatcttttgc accggtgaaa ggggcaagtg cggtgccgct
     1021 ggttggccgt gcttctgttg cctccgatcg ttcaattatt ccgccaggta ctaccttgct
     1081 ggcagaagtg ccgctgctgg ataataacgg caaatttaac ggtcagtacg aattgcgtct
     1141 gatggtggcg ttggatgtcg ggggcgcaat caaaggccaa cacttcgata tctatcaagg
     1201 gatcgggccg gaagccggac accgcgcagg ttggtacaac cactatggac gggtttgggt
     1261 gctgaaaacg gctccgggcg caggtaacgt ctttagcggc tgatgtgata ttctgtatgc
     1321 cgtttttacg gatatgacag gggaggggta acctcacatc ctgattattg actaagtgcg
     1381 cgttgttcat accggatgcg gcgtgaacgc cttatccggc ctacaaatca tgcaaatcca
     1441 aaatattgca ggagttatgt aggcctgata agcgtagcgc atcaggcatt tatgcgttta
     1501 gggtgaggga taacctcgcc ctttttaatt ctgaggtgct atgtctgtgg taattagtga
     1561 tgcatggcgt cagcgttttg gtggcacagc gcgtctgtat ggtgaaaaag cgttgcaact
     1621 gtttgctgac gcgcatattt gtgtggttgg tatcggtggt gtcggttcct gggcggcgga
     1681 agcgctggcg cgcacgggga ttggcgcaat cacgcttatc gatatggatg atgtgtgcgt
     1741 caccaatacc aatcggcaaa ttcacgctct gcgcgataac gtcgggctgg caaaagcgga
     1801 agtcatggcg gagcgcattc gccagattaa cccggagtgc cgtgtaacgg tggtggatga
     1861 tttcgtgacg ccggataacg tagcgcaata tatgagtgcc ggttattcgt acgtgattga
     1921 tgccattgat agtgtacggc ccaaagcggc gctgattgct tattgtcggc gcaataaaat
     1981 cccgctggtc acgaccggtg gcgcgggtgg gcagattgac ccgacgcaga ttcaggttac
     2041 cgatctggcg aaaacgattc aggatccgct ggcggcgaag ttgcgcgagc gcctgaaaag
     2101 cgattttggc gtagtgaaaa acagtaaagg taagctcggc gtggattgcg tgttttctac
     2161 tgaagcgctg gtgtacccgc agtcagacgg tacggtgtgt gcgatgaaag ccacggcaga
     2221 agggccgaag cggatggatt gtgcatctgg atttggcgcg gcaacgatgg tgaccgccac
     2281 ctttgggttt gttgcggttt ctcatgcgct gaagaagatg atggcgaaag cggcgcgtca
     2341 gggttaaaca atcaggtcgg atgagacgcg aacgtcttat ccgacccggt tctgtgctta
     2401 aacctgcttc gtcgcagcga taatcgcctc gcttaacgca tttaacccct ggctgcgtga
     2461 ggcgctaagc tgcgcacgta atcccagctc atcaaacaat gccagcggcg actgtgcctg
     2521 taattcggcg gcggttttcc cctcaacggc agtcaacaac accgccaaca ggccgcgcac
     2581 aatgcgccct tcgctgttgc caaagaagtg cattttgccg ttttcagcca cggtatatcc
     2641 cagccagacg cggttttcgc atccggcaat ctctttagcc tgcgctttta actcgtctgg
     2701 caaagccgga agctgtttcc ccagcatgat caactggcga tatttatctt cccattgcga
     2761 caacggtgcg aaggtattgc gtaacgtttc tgcggttacg gttgtgccga acggatgtcc
     2821 ggcgaattgc gggtttgtca ttaatccacc aataattcca gcgcgcggtc aacggcattc
     2881 accagcgcat ccacatcact ctttgtatta tatggcgcaa atgaggcgcg cagtgtgccg
     2941 gttacgccta attctgccag tagcggctga gcgcaatgtt gcccggcccg caaggcaata
     3001 ccgtactccg ccagtagcgt caccatatcg ctgtgatgaa cgccagcaaa atcaaaggcc
     3061 agcaggctgg aatcctggca gcggaatgaa cgaaagcctg gacgtttcgc cagcgcttct
     3121 tctgcaagcg ttgctaagct acggctccag ctttcggcct ggttgatatc gtaatctgcc
     3181 agccattcca gcgccgcgct taatcctatg acgccagcga catttggcgt tcctgcttcc
     3241 agtttccacg gcgcagattg agtggtgaaa ccgtcaaaac tcacttcgtg aatcattttg
     3301 ccgccaccca gccagggcga catcgcttcc agcagttctg atttgccata cagcacgccg
     3361 atacccgtcg ggccatacag tttgtgacca gagaaagcat agaaatcgat atccagttgc
     3421 tgaacatccg cggggaaatg caccgtcccc tgagcaccat caaccatcac caccatcccg
     3481 gctgaatgag caaaggtaat cgctcgcgcc agatccgggc aaccgccagt cacgttcgac
     3541 atctgaccca acgccagaat ccgactacgg ggagtaatca gctctggcaa caaatcgaca
     3601 tccggcagtc gctgcgcatt aagcggcaat ttcaccactt tggctctagt ttgttgggcg
     3661 accatcagcc aggggacgag gttagcgtgg tgttctgcca cgctgacaat aatctcatcg
     3721 cccggttgca gacgcggacg cgcatagcat tgtgccacca tgttgatgga ttcagtggtg
     3781 ccgcgcgtcc agacgatagt tttatcatcc ggtgcattca gtaattgcgc cactttctct
     3841 cgtgcagctt cataacgcgc ggtcaggcgt tgggcttcgg caaactggct gcgatggacg
     3901 tttccggcgc tcaggctgta aaactgtcga gtggcttcaa ccacggcttc aggtttaagc
     3961 gcggtcgcgg cgctgtcgag atagacgccc gcatcctgta gtgcgggaaa ctgggcgcga
     4021 aactgcgcgg gattaaaaac gttcatggta ctcctcggct tgatgcaccg atcgtgacgc
     4081 aaaattcact ttgatacaag gagagtgcta accatcagaa ttatctggat atcctggcga
     4141 agccgcgtaa gtaacatatg ctgaatagtg ttaggcgtta attttagcct gttaatcaat
     4201 actattaaga tttattaatc gttttaagga gaagatgatg aaaaagactg ccgcaattat
     4261 ttctgcctgt atgctgactt ttgccctgag cgcctgttcc ggttcgaact atgtgatgca
     4321 caccaatgac ggacgtacca tcgtctctga cggcaaacca cagactgata acgatatcgg
     4381 tatgatttcg tataaagacg ctaatggcaa caaacagcag atcaaccgta ctgacgtgaa
     4441 agagatggtc gaactggaac agtaattcgc gatcgcaagg taaaaaaaag caccgcaatt
     4501 aggcggtgct acattaatca ctatggacag acagggtaaa tgtacaggaa gtgaaaaaag
     4561 gtagctttgc taccatggtc tgaatcgcag accaattgca aacacaacaa cacaacatca
     4621 caaccgtaag ccaaaagttc accagaatac gcattccgat aaaacttttc gttccggctc
     4681 aggaagtgcc gccactatag gtatttgctg gtagaagctc aacggacaat ttataatggc
     4741 tcagattaaa aaaactaata ggttacacag tgtgatctaa ttgttaaatt catttaacat
     4801 caaagtttaa aagccatgtc taaacgatta ccaccgctaa atgccttacg agtttttgat
     4861 gccgcagcac gccatttaag ttttactcgc gcagcagaag agctttttgt gacccaggcc
     4921 gcagtaagtc atcaaatcaa gtctcttgag gattttttgg ggctaaaact gttccgccgc
     4981 cgtaatcgtt cactcctgct gaccgaggaa gggcaaagct atttcctcga tatcaaagag
     5041 atattttcgc aattaaccga agcgacgcgt aaactccagg cccgtagcgc caagggggcg
     5101 ttgacggtca gtttactccc cagtttcgcc attcattggt tggttccgcg actttccagc
     5161 tttaattcag cttatccggg aattgacgtt cgaatccagg cggttgatcg tcaggaagat
     5221 aagctggcgg atgatgttga tgtggcgata ttttatggtc ggggcaactg gccggggcta
     5281 cgggtggaaa aactgtacgc cgaatattta ttgccggtgt gttcgccgct actgctgaca
     5341 ggcgaaaaac ccttgaagac cccggaagat ctggctaaac atacgttatt acatgatgcg
     5401 tcacgccgtg actggcagac atatacccga cagttggggt ttaatcatat caacgttcag
     5461 caagggccaa tttttagtca tagcgccatg gtgctgcaag cggctattca cgggcaggga
     5521 gtggcgctgg caaataacgt gatggcgcaa tctgaaatcg aggccggacg tcttgtttgc
     5581 ccgtttaatg atgttctggt cagtaaaaat gctttttatc tggtttgtca tgacagccag
     5641 gcagaactgg gtaaaatagc cgcctttcgc caatggatcc tggcgaaagc cgctgctgaa
     5701 caagaaaaat tccgctttcg ttatgaacaa taatttacgt agggtacgac catgaccagc
     5761 cgttttatgc tgattttcgc cgccattagc ggcttcattt ttgtggcact gggcgctttt
     5821 ggtgcgcatg tgttaagtaa aaccatgggg gccgttgaga tgggctggat ccaaaccggt
     5881 ctcgaatacc aggcgtttca tacgctggcg atcttaggtc tggcggtggc aatgcagcgt
     5941 cgcatcagta tctggtttta ctggagtagc gttttcctcg cgttaggcac ggtgctgttc
     6001 agcggcagcc tttattgcct ggcgctgtcc catctgcgtt tgtgggcgtt tgtcacaccg
     6061 gttggcggcg tgagcttcct tgcgggctgg gcgttaatgt tagttggtgc tatccgttta
     6121 aagcgcaagg gcgtaagtca tgaataaggt tgtattgctg tgccgtccgg gctttgaaaa
     6181 agagtgcgcc gcagaaatta ccgataaagc cggccagcgg gaaattttcg gttttgcccg
     6241 cgtgaaagag aatgcgggtt atgtcattta tgaatgttat caacctgatg atggcgataa
     6301 gttaatccgt gagctgccgt tcagttcatt aatttttgcc cgccagtggt ttgtggtggg
     6361 ggaactcctg cagcatttgc cgccagaaga tcgtattacc cccattgtcg gcatgttaca
     6421 gggtgtagta gagaagggcg gtgaactgcg tgttgaagtt gccgatacca acgaaagcaa
     6481 agagttgctg aaattctgcc gtaagttcac tgttccgcta cgcgctgcct tgcgcgatgc
     6541 tggggtgctg gcgaactatg aaacgccgaa gcgtccggtt gtgcatgtat tctttattgc
     6601 accaggatgc tgctataccg gttactcata cagcaacaat aattcgccgt tctatatggg
     6661 cattccgcgc ctgaaatttc cggcagaggc gccgagtcgt tccacgctca aactggaaga
     6721 ggcatttcat gtgtttattc ctgcggatga gtgggatgaa cgcctggcga acgggatgtg
     6781 ggcggtggat ttaggcgctt gccctggcgg ctggacctac caactggtga agcgcaacat
     6841 gtgggtttat tccgtcgaca acggcccgat ggcgcaaagt ctgatggata ccgggcaggt
     6901 gacgtggctg cgggaagacg gtttcaaatt ccgtccgaca cgcagcaata tctcctggat
     6961 ggtatgcgat atggttgaaa aaccggctaa agttgcggca ttgatggcgc agtggctggt
     7021 taatggctgg tgccgtgaaa ccattttcaa tctcaaactg ccgatgaaaa aacgctacga
     7081 agaagtatca cacaatctgg cgtatattca ggcacagctt gatgaacatg gcataaatgc
     7141 tcagattcag gcgcggcagt tgtatcacga tcgcgaagaa gtgacggtgc acgtccgccg
     7201 catctgggct gcggtgggtg gtcgtcgcga cgagcgataa caggcaaggc cggggcgtcg
     7261 tctccggcct gctacccttt ccgtcaggct gttaccaaag aagttgcaac ctggcgctca
     7321 tcagaaataa tgtgcgttac ctcatccaga tgcccgatgc gggcttcccc tgaacgatta
     7381 aatttacttt tatcaatcaa taacaacgat tgcgcggcac gttttaatag catcgatttg
     7441 taatcagcgt tgatcgcatt ggagtcccac agtgcgccgc tgctatcgat cccttcacaa
     7501 gaaaaaataa acagatcgat ttccagcgat ttaagttggg aaatcagcga gggattgacg
     7561 taacagccat atttgcgctc aagtgtgccg ccggaactga tcagttgaat gcgttcgcgt
     7621 ttaccgagtt catggcaaat cggatggcta ttggtgaaga cctgaatgtt gatgtcaggc
     7681 aactggcgtg ccagatacca gcaagttgaa ctggcgtcta aggctatcac catcccttct
     7741 tcaatccacg cgagcgcctc gcgcgcgata tctgctttat gcgcataatg gcttttcagc
     7801 ctgatgtgaa agggatcgcc actgtcttga ttttgacggt ggatatattt agcgcgtcca
     7861 tgattgcgca gaattttacc ctgcgtctgt aattcattga gatcgcgacg aatggtttct
     7921 ttactgacct ttagctgttc agagagagct tccgtggtca ggctggtatg gttcagcagc
     7981 aggtcgacta tcgcttgctg gcgtgccgct ttcatatttt tcaccccatc attcgataac
     8041 cgcccgcgtc atgcgggcgt accggtatga gattacggtg ttaccccttt ttttaaaaga
     8101 atattcccgt acttcgctcg ttcgcctgtg ataacgatcg caaaggcttt ttgcgcccgt
     8161 tcataaaacg caaaacgatt gatgcggatg atgtcaggac acggggcttg tagtgaaagc
     8221 gcattacggt aacgtcgttc tacttcagga tcgagagtgt caccctctac cgccgccatc
     8281 atcaccagcg gcggggcata actgtccagc tcaaataacg ggataatcgc ctggagcaag
     8341 tcgctcacca acaggccatc agcgcggatc acctgcggtt ccatcgaatg ggcgggaaag
     8401 tgagcatcgg aaaaaataat ttcatctcca tgtcccatct ctgccagcac tttcaatagt
     8461 tcgggagaaa ttaacggcga aattgttttc agcatttcac acttcctcta taaattcagg
     8521 ttcagtttgc gggtagaaat aacggtactg ataatgaatc tgtgcacggg cttcttccgg
     8581 gctgttaaat tcccctacgc cataccaacc gaacagcgca gctcctgcga cggtcgtttc
     8641 ggcatcgtcg agaactttta ccggaatatc aagcatattg gctttaatct gattccacaa
     8701 tgtgttgcga cttcctccac cgactaacaa taattcatag gctttaaagt gcccgatttt
     8761 ttccagcatc tgtagattgc gctgtaattg cgcagttaac ccttccagcg ccgcgcgata
     8821 gaaatgcccc cgcgtggtat taagcgtcac tccttgccag ccagcgttct gacacgacaa
     8881 taaatcacac tgcattttca caccatccgc gccaggtgcg atcagacgag cttcttcaat
     8941 caacatttgc cagggtgttt cagccgtcca gaacagtttt ctcacccatt ccagcacgcc
     9001 ggatgccagc cattgcatac ctgggttata caaccatgcc tggctatcta gttcgcaggt
     9061 ggaaccggcg tactgactta acagcgaagt atcaacctag gcgctgcgaa ccattaaaat
     9121 ttcccatgta ccggaggaga gcacgggttc attttgttcg gcaccagcgc caaaaagggc
     9181 gaactgggta tcgtgacctg cggaaatcac cggtatgcca acgggtaagc cgagcattgc
     9241 tgcggcgttg ttctgtagcg taccaatctg ttcacccgct tccaccagac gagggaagag
     9301 tcggcgtgga ataccggtgg cttgtaaaat ttgcggactg aaatcgcgtt gctggatatc
     9361 cagcatctgg ctggttccgg ccatcgtgat atcagtagtg aattcgctgg ttaaacggtg
     9421 gttaatcagc gacgaaatga agagccaggc gtgcgcgcgt tccagcagtt gtggatgatt
     9481 ttctttcaac cacaccaact tatataacgt attgaaacta aaggctccga cgccagaaat
     9541 agcctgcaac cgctgtgcgg agattaaccg ttcaatattg tccataaccg ctgctgttcg
     9601 cggacatttc cagctaatga tcggatagag cagattgcct tgcttatcta ccagagcgcc
     9661 atccacacta aaggtggtga cggcgatacc gcggatgtgg cattcagtca gttcactatt
     9721 gatttgccga cagcaatcag caaagcgttg caaaatggca gtctaaagac cactggtgcc
     9781 aggtgttgtt ttccatcgcg atatcgctgg cattaggcgt tgaggcgcgg gcaacaattt
     9841 ttccctgccg attaaccgcg atggccctga cattggtcgc gccacagtcg agtaccagga
     9901 taacttcttg tttcataatc gctcctgcaa tatagccgga taacattgtt tatccggcta
     9961 accactcttg cttgttcagg cccggtagcc ggagcgaccg ggcatcacat cagggtgtaa
    10021 tgtattaacg cttgtacaac ggaccgtagt tctggcaagc gcggtaatcc tggccttcaa
    10081 tatccatgcc gtgcgcagcc caggcagaag gacgatacac tttggtctct tcaacgttgt
    10141 gcatacatac cgggatacgc agcatggagg caagggtgat aaagtctgcg ccaacgtggc
    10201 cgatggtcag aactccgtgg ttggcacccc agttcgccat taccgaatac acatccgtaa
    10261 acggcccttt accagtgagg cgcggtgcaa accaggtggt tggccaggtt gagttggtgc
    10321 gtttgttgag gatgtcatgc acatccttcg gcaattccac gctccagcct tccgcgattt
    10381 gcagtaccgg tcccaggcct ttgatgatgt tgacacgagt catggtgaac gggacgccgc
    10441 cttcggtaag gaagcgggaa gagtaaccgc cgccacggaa gtattcgtgg atcgccgggc
    10501 accattcggt agcggcgagg caagcgtcag cctcttgctg agagatttcc cagtgcggct
    10561 tcatcgtcgg gttaccttcg ctgtcgcgtt gtttacagga accgtccagc gcagcagaac
    10621 ctgagttgat caaatggatg atgccgtgtt ctgccagtcc gtccagttta tgccccgtta
    10681 cacgctcaat tgcttctggt gaccagtagg tacgcacatc ggcaaatacc tgagcggtgc
    10741 cggtgagctg gtgacccatt agcattgcca cgccgttaag actgtcgttt tcggtcgcca
    10801 cgacaaaggg ttcgcgcacg ccattccagt caaatgaact gttgaggatc gcttcggcgg
    10861 tgtcaccatt gggatattga tcggtccagt gacgttgccc ctggaagccc gcagcgatgg
    10921 cgttgtagcc aagtgattct tccacgcgac caatatcggc cagtttgctg ttgccttgca
    10981 tcatgtcgcg gatacacatc gccatcagta aactttcgcg cagaactgcg cggctttgct
    11041 cggcattgcg ttgatactgt ttgttatttt catcttcgcc atagcggaag tttttatcag
    11101 cccaggccag tgccatttcc agttcggctt cgtcgtaaat cttctgatcg atacggcgac
    11161 gcagttcggt catatccacc gcctggactt tcattcccag ccaggattca aagaagttgt
    11221 gatcaacaat agaaccggcg atacccatcg aaacgccgcc cagcgacaga tagcttttac
    11281 ctttcatgct ggcgacggcc aaaccggcgc gggcaaagcg cagcagtttt tcttcaacat
    11341 cggcaggaat cgatgtgtca tcggcatcct gaacgtcatg accgtaaatg gagaatgccg
    11401 ggatgccttt ctggctgtga gctgccagag ccgcagccag gtaaacagcg ccggggcgtt
    11461 cagtgccgtt aaagccccaa atggctttcg ggcgggttgg atccatgtcg atggtttcac
    11521 tgccatagca ccagcaaggc gttaccgtaa tggtgaggcc tacattctgg ctgctgaatt
    11581 tttcttcgca agcagcggct tcagccatac ccgcgataca ggtatcggaa atgacacact
    11641 cgacggcagc tccgcaggca tggcgcagtt tctcggtcag cagtgcggcc gtagctttcg
    11701 ccatattcat tgtttgttct tcaagcgact cacgaacacc catgcgacga ccgtcaataa
    11761 ccgggcggat accaattttc ggtaagctga tttttttcat tacagattcc tcactttatt
    11821 cggaaaatat gttcagttag ttgccgtttg agaacggaaa cgggcaaaga taaagatgac
    11881 cgcgaagcag agtgcgggga tcagttcagc agtggggatg ttgcccgccg cgtcactgac
    11941 aaaacccatg accggagtga caataccgcc gccaataatg gtcataacga tgaaggacga
    12001 accgtatttg gtgtcctggc cgagattctt aatgcccagc gagaagattg ttgggtactg
    12061 aatcgacata aaggcgctgc ataaagtcag ggctattaag cccacatgac cgccagcgaa
    12121 ggctgagatc aggcacagtg ccatagcgat taatgcgtag gcggccagga ctttgtgtgg
    12181 tgcgaagcga ctgatgagcc aggtaccggt gaaacgacca ataaagaagc acaccatggt
    12241 tccggttaaa tagttagcgg caaagcctgc agtcatacct agaatttctt ctacagcgta
    12301 gcgaatcaaa tagctccagc aggccgtttg tgcgccgaca tagcagaatt gcgctaatac
    12361 cgcccagcgc cagtgacgaa tacgcgccag acgagaaagc gatgcggaga acgatccttg
    12421 tttggcgtca ctgtgattat cactctgcaa tgccgggaat ttcgtcagca tgatcagcag
    12481 ggcgaccagt aacacgatag ccacgatgat catataaggt gtctgtaccg ataataccag
    12541 gctgtgttta tacgcactca attgctctgg agacatttta tcgagaacgt cttgcgattg
    12601 atgtggcacg ttagacaaaa taagactttg cccaaagaca accgcgataa ttgcgccaaa
    12661 cgagttaaat gtttgcgcaa gatttaagcg gaagtgacta ctgctttccg gccctaatac
    12721 cgtaacaaaa gggtttgcgg cagtttccag acaacctaat ccggctgcaa taataaatag
    12781 gccaactaaa aataaggtgt agttcattat ttctgcagcg ggccagaata atgcagcgcc
    12841 caaggcatat aaaaataatc cggtaataat ccctgcttta taactgagtt ttttcatcaa
    12901 tatcccagca gggattggga taatgaaata accaaagtaa aaggccgatt ggatcaggcc
    12961 agcctggaaa tttgtcagcg taaaagcctg ctggaattga ggtaataaaa tgtcgttaag
    13021 gttattggct accgcccaaa gaaaaaacag tgagcacagc agcgcgaatg gaataatgta
    13081 acttctgctt tgccctgcat ctttatctac cgcacggtaa ctctgcgttt gtattgatgt
    13141 gtttcccata gcatcctctt aggttcagaa gcttaagcac gtaatcggtt atgttttcaa
    13201 aagtgcaaaa ccagcgagtt cgggcttcat atcggtgaat atttgcctgt ctgaaatgaa
    13261 ctcatggatt cattttcaaa taaaaaacgc tctgtaatat gacggcggtc acacttatta
    13321 gctggaaggt gactttatgt gactaccatc actttaataa tcgaaatttc atgcccgttt
    13381 cagaaaataa atcataaaaa acacaaacca gatgaccgta ttaattgttt gtgccgaaaa
    13441 cgggcatttc tggcgagggg aaatggaaaa atgaccgttt tgataaatat ttttaagagc
    13501 ggtcattttt aacggggtta taaaaagaga acttgcagtt atttactgtg attagttgag
    13561 ctaggtcaca aaatataagc ttgtgaattt ctttcttgtt aatcaggaag aagatggtgg
    13621 gtaattaaac ggctaattca atagtgtgaa aggaacaaca ttattgccct gttttgaatc
    13681 agagagaggt agctaatgga acgaaataaa cttgctcgtc agattattga cacttgcctg
    13741 gaaatgaccc gcctgggact gaaccagggg acagcgggga acgtcagtgt acgttatcag
    13801 gatgggatgc tgattacgcc tacaggcatt ccatatgaaa aactgacgga gtcgcatatt
    13861 gtctttattg atggcaacgg taaacatgag gaaggaaagc tcccctcaag cgaatggcgt
    13921 ttccatatgg cagcctatca aagcagaccg gatgccaacg cggttgttca caatcatgcc
    13981 gttcattgca cggcagtttc cattcttaac cgatcgatcc ccgctattca ctacatgatt
    14041 gcggcggctg gcggtaattc tattccttgc gcgccttatg cgacctttgg aacacgcgaa
    14101 ctttctgaac atgttgcgct ggctctcaaa aatcgtaagg caactttgtt acaacatcat
    14161 gggcttatcg cttgtgaggt gaatctggaa aaagcgttat ggctggcgca tgaagttgaa
    14221 gtgctggcgc aactttacct gacgaccctg gcgattacgg acccggtgcc agtgctgagc
    14281 gatgaagaga ttgccgtagt gctggagaaa ttcaaaacct atgggttacg gattgaagag
    14341 taatttcgta aagcaacaag gagaaggatg atggctaaca gaatgattct gaacgaaacg
    14401 gcatggtttg gtcggggtgc tgttggggct ttaaccgatg aggtgaaacg ccgtggttat
    14461 cagaaggcgc tgatcgtcac cgataaaacg ctggtgcaat gcggcgtggt ggcgaaagtg
    14521 accgataaga tggatgctgc agggctggca tgggcgattt acgacggcgt agtgcccaac
    14581 ccaacaatta ctgtcgtcaa agaagggctc ggtgtattcc agaatagcgg cgcggattac
    14641 ctgatcgcta ttggtggtgg ttctccacag gatacttgta aagcaattgg cattatcagc
    14701 aacaacccgg agtttgccga tgtgcgtagc ctggaagggc tttccccgac caataaaccc
    14761 agtgtaccga ttctggcaat ccccaccaca gcaggtactg cggcagaagt gaccattaac
    14821 tacgtgatca ctgacgaaga aaaacggcgc aagtttgttt gcgttgatcc gcatgatatc
    14881 ccgcaggtgg cgtttattga tgctgacatg atggatggta tgcctccggc gctgaaagcc
    14941 gcgacgggtg ttgatgcgct cactcatgct attgaggggt atattacccg tggcgcgtgg
    15001 gcactaaccg atgcactgca cattaaagcg attgaaatca ttgctggggc gctgcgagga
    15061 tcggttgctg gtgataagga tgccggagaa gaaatggcgc tcgggcagta tgttgcgggt
    15121 atgggcttct cgaatgttgg gttagggttg gtgcatggta tggcgcatcc actgggcgcg
    15181 ttttataaca ctccacacgg tgttgcgaac gccatcctgt taccgcatgt catgcgctat
    15241 aacgctgact ttaccggtga gaagtaccgc gatatcgcgc gcgttatggg cgtgaaagtg
    15301 gaaggtatga gcctggaagg ggcgcgtaat gccgctgttg aagcggtgtt tgccctcaac
    15361 cgtgatgtcg gtattccgcc acatttgcgt gatgttggtg tacgcaagga agacatttcg
    15421 gcactggctc aggcggcact ggatgatgtt tgtaccggtg gcaacccgcg tgaagcaacg
    15481 cttgaggata ttgtagagct ttaccatacc gcctggtaaa tgcgctgatg tgataatgcc
    15541 ggatacgatt tgcggcgtat ccggctcgcc gttaccgtac caaccgcaat tgctgtaaat
    15601 tgccgtcgat atgcaaatcg gtttgtaagc gggcaatatc gcggcacaga aacgccatct
    15661 ctttatgggt ttctaatttt ttgcgccact tttcggcaac cgcatccaga ttctcatata
    15721 tcccttccag actctgaaac tcgaccagca gctgcgtggc gctttttggt ccgattcccg
    15781 caacacccgg tacctttgaa ctgctgatcc ccgcaagtcc ccagtaatcg ggcaactgct
    15841 gcggttgaac gccaaattct ttatcgataa atggcgcatc cagccaacgt ttctggaagt
    15901 aatcacgaat acgtaatgtc ggtgaaagta actgacagta gcctttatct gtcgaaacaa
    15961 tcgttgcctg atgcccggcc tgtgtcactt tgaccgccag tgtggcggct aagtcatcgg
    16021 cttcgttgcc gctggctgac cagcacggga cgccgcgttg ctcaaaggcg gcgcgtaatg
    16081 caggcatctc gtcgtgcaac tcttccggca ttggcggtcg atccgctttg taatctggta
    16141 aacgctgatg acgccagccg ctactgcggt tttcatcatc aaaaacggcg accgcgtggg
    16201 ttggctggct gtgcataatg agctgatcga gcgcatgctg gcaggtttcg acacagggcg
    16261 aaccctgaac ggcatgaatg cgacggataa gattcagtgc atcgacaata agcaaatgaa
    16321 cagccacgat aaccccctta aagatcaggc gataagggta acattcgatg ccggaacagg
    16381 ctatggctgg ttgagaaaac aggaagaggc ttcgcaaaac gaggcctctg ggagagatta
    16441 atcgcaggca acgatcttca tcgccaggcc gccgcgagag gtttcgcggt acttggcgtt
    16501 catgtcttta cctgtttcgt acatggtttc gataacttta tcgaggcaga cgcgcggttc
    16561 gctggtacgg cgcagtgcca tacgcgcggc gttcaccgct ttcactgctg caatggcgtt
    16621 acgctcaatg cacggtacct gaacctgccc cgcgaccggg tcacaagtca gaccgaggtt
    16681 gtgctccatc gcgatttccg ctgcgatgca aacctgcgcc gggcttgcgc ctaacagttc
    16741 tgccagacca gctgccgcca tagagcacgc cacgccaact tcaccctggc aacccacttc
    16801 ggcaccagaa atcgacgcgt tcatcttata aagagaacca atggcgctgg ctaccagcag
    16861 gtaacgagcc agtgagttag cgttcacttc gcggataaac ttatcgtagt acgccagcac
    16921 agccgggata atcccgcacg caccgttagt cggcgcagtc accacgcgac cgccagcagc
    16981 gttctcttcg ttcactgcca gtgcaaacat gttgatccag tcaacaaccg ccatcgggtc
    17041 agtggtggtt ttatcctggc tgaccagcat ccggcgtagt gccgcagcac ggcgtggaac
    17101 gcgcagtttg ccaggcaaca cgccttcggt ggaaataccg cgctcaatac cgccacgcat
    17161 gacttcccag acgttcgcca ggtgctgttc cagctcttct ttactgtgca gcgccagctc
    17221 gtttttcatc atcaggccag agagtgacag cccggtttcc tgacaatgtt tttgcagatc
    17281 ggctgctgaa ctgtacggat aaggaacttc aaccggtgcg ctatcctgct ggccaaaatg
    17341 ctcttcatca acgataaagc caccgccaat agagtagtaa gtctggctgt aaacgacttt
    17401 atcgcccgcc agcgcggtaa tgcgcatacc gttttcatgc agagaaaggt tgtctgcatg
    17461 gaagttcatg cactgatcaa ccgggaactc cacttcatgc tgaccgtttg ccagcatcag
    17521 gcgaccatga gtattcacat cctgaataaa accggggatg gaatcgatat ccacggtatc
    17581 cggcaggtta cccgccaggc ccataataat ggcgatatct gtgtggtggc ctttaccagt
    17641 cagagagagc gagccgtaca cgtcaaccac cacgcgggtc acgtctttaa gcaggttacg
    17701 ggcaatcaga tcgtcggtaa attgtttacc cgctttcatt ggtccaacgg tatgagaact
    17761 ggaagggcca atgccgattt tgaaaatatc gaatacgcta atcataggaa atacatcgcg
    17821 ttaaaacgga ggaagcgccg cccgaaagcg gcgcgaaagg acttagctga acagagagta
    17881 gaagattgcg gagattgcaa tcagacccat cacgacaacg aatacgttgc tgatgtgacc
    17941 gctgtactta cgcattgccg gtactttctg aattgcgtac atcggcatca ggaacaggat
    18001 catcgcgatg attgggccgc ccagggtttc aatcataccc aggatgctcg ggttcagggt
    18061 ggcaacaatc caggtcgtta ccagcatgaa cagcgcagtg atacggttca gcttgttgat
    18121 ttcgatagac ttacctttac cacgcagaga tttaatcacc ataccgttga agccttcacg
    18181 tgcgcccagg tagtggccga ggaaggattt ggtgatagcg ataatcgcga taatcggagc
    18241 catccacgcg ataaccggtg cgttaaagtg gttagccagg taagacagaa tcgagatgtt
    18301 ctgctcttta gccgcagcca ggtctgccgg agtcaggctc agtacgcagc tgaagacgaa
    18361 gaacattacg gtcagcacca tcatgatgtg tgcgaatgcc aggatcttcg agcatttctg
    18421 ttctgccata tcgccgtact cttcacgctt cgcaacggcg aaagaagaga tgatcggaga
    18481 gtggttgaac gagaacacca ttaccggaat tgccagccac agggtcatcc acagaccgtt
    18541 tccggttgca gatgcagtgt ccagagacag cgtttccagt gctgcgccgt tccactgcgg
    18601 gatcaggtac agagccagca gcatcagtac gccaacaaac gggaatacca gaatactcat
    18661 cgctttaacg atcatctgct caccaaagcg aacgatggtc atcataccca cgatcaggat
    18721 cagcgacaga atcgcacgcg gcggtggcgt catacccagc tggtgagaca tgaagctttc
    18781 aacggtattg gtgattgcca cgctataaac cagcaggatc gggtagatag cgaagaagta
    18841 gagcagggta atcagtttac ctgcgccaat accaaagtgt tcttctacaa cctcggtgat
    18901 gtcttcgccc gggtttttac cagacagtac gaagcgagtc aggccgcggt gagcaaaaaa
    18961 cgtcatcggg aacgcaagga tagccatgat gatcagcggg atcataccgc caacaccggc
    19021 gttgattggc aggaacagca cgcccgcgcc gattgccgtg ccgtaaaggc ccagcatcca
    19081 catggtgtct gtcttgcgcc aggcactacg agagtctttc gacgcaatcg tgctggtttg
    19141 agtcgtttcc atctatttct cctggaggaa tgcaaataat tcagctttta gccagttcag
    19201 cccgggaagg tgcctgaccg taaaaatgaa atctgtctat cggcgatggc ttaatcattt
    19261 accgccgtaa taatttgagg gcggaaagat acacacaagt tatgtatcta tcagtgatct
    19321 tgatcccaaa tacaataatc tgcttggaag atagattatc ccaacgcttt actctgtaaa
    19381 tcaaaagtaa aagagaattt aagggcaata tttgcccatt aaacgacatc tgacttcttc
    19441 cggggcgcgc aatatatgta ggttgaactg aaaaaatcaa tacttttagt taatcatggc
    19501 gataatttct ttttcatagc gataaaatac ggtttatgaa aatagggaaa cgtttgtgtt
    19561 gcaacataaa ttgtatgaca tattaactgt gatgatattt gtcgcgtgag ggtggtaaga
    19621 aacattcata taacaaatga aatgagttga tgagtaagtg accaaaaacg agaaaacccg
    19681 gcaacaaacg tcaccgg
//
LOCUS       DAJTSI010000075        19336 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000075 DAJTSI010000000
VERSION     DAJTSI010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.075"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..2095
                     /gene="pbpC"
                     /locus_tag="OO327_002358"
     CDS             <1..2095
                     /gene="pbpC"
                     /locus_tag="OO327_002358"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCR6018878.1"
                     /translation="ARYLEALINYEDRWFWKHPGVNPFSVARAAWQDLTSGRVISGGS
                     TLTMQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILYLNRAPFGGTLQGIGA
                     ASWAYLGKSPANLSYSEAAMLAVLPQVPSRLRPDRWPERAEAARNKVLERMAAQGVWS
                     REQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTLDAGLQRRLEELAQNW
                     KGRLPPRSSLAMIVVNHTDMRVRGWVGSVDLNDDSRFGHVDMVNAIRSPGSVLKPFVY
                     GLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPINMSEALVRSLNLPAVQVLEA
                     YGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAAAYTAFARHGKAGKLR
                     LQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPLAWKTGTSYGYRDAWA
                     IGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLSRSANLPEDPRPNSVT
                     RGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEGINGIRFPIWLDDKGQ
                     RVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICPPYGHDAQLPLQLTGV
                     RDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRNVTLHLTDKGDYQLLV
                     MDDVGQIATVKFVMQ"
     gene            2245..2676
                     /gene="ndk"
                     /locus_tag="OO327_002359"
     CDS             2245..2676
                     /gene="ndk"
                     /locus_tag="OO327_002359"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR6018879.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2826..3980
                     /gene="trmG/rlmN"
                     /locus_tag="OO327_002360"
     CDS             2826..3980
                     /gene="trmG/rlmN"
                     /locus_tag="OO327_002360"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR6018880.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4265..5278
                     /gene="rodZ"
                     /locus_tag="OO327_002361"
     CDS             4265..5278
                     /gene="rodZ"
                     /locus_tag="OO327_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR6018881.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5305..6432
                     /gene="ispG"
                     /locus_tag="OO327_002362"
                     /gene_synonym="gcpE"
     CDS             5305..6432
                     /gene="ispG"
                     /locus_tag="OO327_002362"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR6018882.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6535..7809
                     /gene="hisS"
                     /locus_tag="OO327_002363"
     CDS             6535..7809
                     /gene="hisS"
                     /locus_tag="OO327_002363"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR6018883.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7827..8447
                     /gene="yfgM"
                     /locus_tag="OO327_002364"
     CDS             7827..8447
                     /gene="yfgM"
                     /locus_tag="OO327_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR6018884.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALTGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8458..9636
                     /gene="bamB"
                     /locus_tag="OO327_002365"
     CDS             8458..9636
                     /gene="bamB"
                     /locus_tag="OO327_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR6018885.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9754..11226
                     /gene="der"
                     /locus_tag="OO327_002366"
     CDS             9754..11226
                     /gene="der"
                     /locus_tag="OO327_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR6018886.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11296..11511
                     /locus_tag="OO327_002367"
     CDS             11296..11511
                     /locus_tag="OO327_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR6018887.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="OO327_002368"
     CDS             complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="OO327_002368"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR6018888.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13040..14506
                     /gene="guaB"
                     /locus_tag="OO327_002369"
     CDS             13040..14506
                     /gene="guaB"
                     /locus_tag="OO327_002369"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR6018889.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14575..16152
                     /gene="guaA"
                     /locus_tag="OO327_002370"
     CDS             14575..16152
                     /gene="guaA"
                     /locus_tag="OO327_002370"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR6018890.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16247..16786)
                     /locus_tag="OO327_002371"
     CDS             complement(16247..16786)
                     /locus_tag="OO327_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR6018891.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16802..17320)
                     /locus_tag="OO327_002372"
     CDS             complement(16802..17320)
                     /locus_tag="OO327_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6018892.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17631..17822)
                     /locus_tag="OO327_002373"
     CDS             complement(17631..17822)
                     /locus_tag="OO327_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCR6018893.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17840..17992
                     /locus_tag="OO327_002374"
     CDS             17840..17992
                     /locus_tag="OO327_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018894.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            18174..19313
                     /gene="pdeF"
                     /locus_tag="OO327_002375"
                     /pseudo
     CDS             18174..19313
                     /gene="pdeF"
                     /locus_tag="OO327_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
BASE COUNT         4553 a         4705 c         5259 g         4819 t
ORIGIN      
        1 ggcacgttac cttgaagcgc tgatcaatta tgaagatcgc tggttctgga agcatccggg
       61 ggttaatcca ttctcggtgg cgcgcgcagc atggcaagat ctcacttcgg gacgggttat
      121 ttccggtggc agcacgctca ctatgcaggt tgctcgtctg cttgatcctc accccaaaac
      181 atttggtggc aaaattctcc agctctggcg cgcgttgcaa ctggagtggc atttgtctaa
      241 gcgtgaaatt ctgatcttgt atcttaaccg cgctccgttt ggcggtacgt tgcaggggat
      301 cggtgcggca agttgggctt atctcggaaa atcgcctgcg aatttaagct attccgaggc
      361 ggcaatgctg gcggtgttac cgcaagtgcc cagccgactg cgcccggatc gttggccgga
      421 gcgtgccgaa gccgcgcgta ataaagtgct cgaacggatg gctgcgcaag gtgtgtggtc
      481 ccgtgagcag gtaaaagagt caagggaaga acccatctgg ctggcacccc gacaaatgcc
      541 gcaactggca ccgctgtttt cgcgcatgat gctcggtaaa agcaaaagcg acaaaatcgt
      601 tactacgctg gatgccggtc ttcaacgacg tctggaagaa ctggcgcaaa actggaaagg
      661 gcggttgcca ccgcgcagct cactggcgat gatcgtggtt aatcataccg atatgcgtgt
      721 tcgcggctgg gtgggatcgg ttgatctcaa cgatgattca cgctttggtc atgttgatat
      781 ggtcaatgcg atccgatcgc caggatcggt gctcaaaccg tttgtttatg gtctggcgct
      841 ggatgaaggc ttgatccacc cggcatcact gctgcaagac gtcccccggc gcaccggtga
      901 ttatcgacca ggtaactttg atagcggttt tcatggcccg atcaacatga gcgaggcgct
      961 ggtgcgctcg ctgaacttac ctgctgtgca ggtgctggaa gcctatggac cgaaacggtt
     1021 tgcggcaaag ttacgcaatg ttggattgcc gttatatttg cccaacggcg ctgcgccgaa
     1081 tctttcactc attctcggcg gcgctggtgc aaaactggaa gatatggcgg cagcgtatac
     1141 cgcgtttgct cgccacggca aggcaggcaa attgcgctta cagcctgacg atccgctgct
     1201 tgaacggcct ttaatgtcgt cgggggcggc gtggatcatt cgccggatta tggctgatga
     1261 agcgcaaccc ttgccggata gtgccttgcc gcgcgtcgcc ccactggcat ggaaaacggg
     1321 caccagctat ggctatcgtg acgcctgggc gattggggtt aacgctcgct atgtcattgg
     1381 gatctggact ggcagacctg acggcacgcc cgttgttggt cagtttggct ttgccagtgc
     1441 cgtaccattg ttgaatcagg tcaataatat cttactgtcg cgcagtgcga atctgccgga
     1501 agacccgcgc ccgaactcag tcactcgtgg cgttatctgc tggccgggcg gacagtcttt
     1561 gccggaaggt gacggtaact gtcgccgccg cctggcaacc tggctgctgg atggcagcca
     1621 gccgccaacg ctgttactgt cagagcagga agggattaat ggcattcgct tccccatctg
     1681 gctggatgac aaagggcagc gcgtcgccgc tgactgcccg caggcgaagc aagaaatggt
     1741 aaatgtctgg ccgctaccgc tggaaccctg gctgcccgca tcagaacgtc gcgctgtgcg
     1801 cttaccaccg gcctcgacaa tttgcccgcc atacggtcac gatgcccagc tccctctgca
     1861 gctgacaggc gttcgcgatg gcgcgattat taaacgttta ccgggcgcag cggaagcgac
     1921 tttgccgttg caatcaagtg gaggggcagg tgaacgctgg tggtttctga atggcgaacc
     1981 gttaactgaa cgcgggcgca acgtgaccct gcatttgacg gataaaggtg attatcaatt
     2041 gctggtgatg gatgatgtgg ggcaaatcgc gacagtgaaa tttgtcatgc aatagtctgt
     2101 tttgtccctg attgttgcta aaactcattt tattttaaaa aaaatgttac cttcatcaat
     2161 agtcaacggc cctgttgctc attataatcc gcgccatctc gtacgctggt acagacaaca
     2221 acagaacaat ttacagaggt aaaaatggct attgaacgta ctttttccat catcaaaccg
     2281 aacgcggtag caaaaaacgt cattggtaat atctttgcgc gctttgaagc tgcagggttc
     2341 aaaattgtcg gcaccaaaat gctgcatctg accgttgaac aggcacgtgg cttttatgct
     2401 gaacacgatg gaaaaccgtt ctttgatggt ctggttgaat tcatgacctc tggcccgatc
     2461 gtggtttccg tgctggaagg tgaaaacgcc gttcagcgtc accgcgatct gctgggcgcg
     2521 accaatccgg caaacgcact ggctggtact ctgcgcgctg attacgctga cagcctgacc
     2581 gaaaacggta cccacggttc tgattccgtc gaatctgccg ctcgcgaaat cgcttatttc
     2641 ttcggcgaag gcgaagtgtg cccgcgcacc cgttaataat ttcgtaaatg ccgcgtgcaa
     2701 acgtggcatc cgtgcgccag aatttgtaca atgcagcgcc cccggacgag cagccgctca
     2761 ccggggcgtt tcttttttca accctccagg ggccataacg tgtaataacg aggccggaat
     2821 agcatatgtc tgaacaatta gtcacacctg aaaacgtcac cacgaaagat ggaaaaatca
     2881 acctgctgga tctcaaccgt cagcagatgc gggagttttt taaagattta ggtgaaaaaa
     2941 ccttccgcgc cgatcaggtg atgaagtgga tgtatcacta ttgctgcgac aactttgatg
     3001 agatgaccga catcaacaaa gtgttgcgcg gcaaactgaa agaggtggcg gaaatccgcg
     3061 caccggaagt ggttgaagaa cagcgttcat ccgacggcac cattaaatgg gcgatcgccg
     3121 ttggcgatca gcgcgtcgaa acggtgtata tcccggaaga cgaccgtgcc acgctctgcg
     3181 tctcttcgca ggtggggtgt gcgctggagt gtaaattctg ttccaccgcc cagcagggct
     3241 ttaaccgcaa cctgcgggta tcggaaatta ttggccaggt gtggcgtgcg gcgaaaatcg
     3301 tcggcgcagc aaaagtcacc ggtcagcgcc caatcactaa cgtcgtgatg atgggcatgg
     3361 gcgagccgct gctcaacctg aacaacgtcg ttccggcgat ggaaatcatg cttgatgatt
     3421 tcggttttgg cctgtctaaa cgtcgcgtga cgctttccac ttccggtgta gttccggcgc
     3481 tggataaact gggcgatatg atcgacgttg cactggcaat ttccctgcac gcgccgaacg
     3541 acgaaattcg tgacgaaatt gtgccgatca acaaaaagta caatatcgaa acgttccttg
     3601 ctgcggtgcg ccgttatctg gagaaatcca acgccaatca gggccgagtc actattgaat
     3661 acgtgatgct tgaccacgtt aacgacggca ctgaacacgc gcaccaactg gcggaactgc
     3721 tgaaagatac gccgtgtaag atcaacctga tcccgtggaa cccgttcccg gacgcgccgt
     3781 atggacgcag ttcgaacagc cgtatcgacc gtttctcaaa agtgctgatg agctacggtt
     3841 tcaccactat tgtgcgtaaa acgcgtggtg atgatatcga tgctgcctgt ggtcagttgg
     3901 cgggcgatgt tatcgaccgt acgaaacgta ctctgcgtaa acggatgcag ggtgaagcca
     3961 tcgatattaa agcggtctga tagccattac gccacggtta ctttatgtac cgtggcgtaa
     4021 tcttttatga atgaagtatt aagttgcaat tgtctggtca atattaacgg tgcgtttttg
     4081 ctgactttaa ggcagtatgt aacgatgcaa cagtaagtta gcctcagtga atgtgggctt
     4141 tgtcacgagc acacagacgg tcttatactg tatgataacg gttaacttaa cggatgtttc
     4201 gcggtgtggg tgagcatgat ggttcaccgg catctcaatt ctcatttaaa cgtacctgca
     4261 gcgaatgaat actgaagcca cgcacgacca aaatgaagca cttactaccg gcgctcgcct
     4321 gcgtaatgct cgcgaacaac taggacttag tcagcaggcc gttgccgagc gactttgcct
     4381 gaaggtttcc acggtacgcg acattgaaga agataaggca cccgccgatc ttgcttcaac
     4441 attcctgcgc ggatatatcc gctcttatgc gcgtctggta catattccag aagaagaact
     4501 gctgccaggg ctggaaaagc aggctccact tcgggctgca aaagttgcgc caatgcagag
     4561 tttttccctc ggtaaacgcc gcaaaaaacg cgacggctgg ctaatgacct tcacctggct
     4621 ggtgttgttt gtggttatcg gcctgagcgg tgcctggtgg tggcaagacc acaaagctca
     4681 gcaggaagag atcaccacta tggccgatca atcttcggcg gaactgagca gtaatagcga
     4741 gcaggggcag agtgttccgt taaatacgtc gacaactaca gacccggcta caacgtccac
     4801 gccgccagcg tctgtggata ctaccgcaac caacacgcaa acacctgccg taactgcgcc
     4861 agcaccagct gttgatccgc aacagaatgc ggttgtttcg ccttcgcagg caaatgttga
     4921 taccgccgcg accccggtac caacggcagc aacaacgcca gatggtgctg cgccgttgcc
     4981 aaccgatcag gctggcgtga ccacgccggc ggctgatccg aatgcgctgg tgatgaactt
     5041 tactgccgat tgctggctgg aggtcactga tgctaccggt aaaaaattgt ttagcgggat
     5101 gcagcgtaaa gacggtaatt tgaatttaac cggccaggca ccgtataaac tgaaaattgg
     5161 tgcgccagcc gcagtacaga tccagtatca agggaaacct gtcgatctga gtcgttttat
     5221 cagaactaac caggttgcgc gtctgaccct caatgccgaa caatcaccgg cgcagtaaca
     5281 gacgggtaac gcgggagatt tttcatgcat aaccaggctc caattcaacg tagaaaatca
     5341 acacgtattt acgttgggaa tgtgccgatt ggcgatggtg ctcccatcgc cgtacagtcc
     5401 atgaccaata cgcgtacgac agatgtcgaa gcaacggtca atcaaatcaa ggcgctggaa
     5461 cgcgttggcg ctgatatcgt ccgtgtctcc gtaccgacga tggacgcggc agaagcgttc
     5521 aaactcatca aacagcgggt taacgtgccg ctggtggctg acatccactt cgactatcgc
     5581 attgcgctga aagtagcgga atacggcgtc gattgtctgc gtattaaccc tggtaatatc
     5641 ggtaatgaag agcgtattcg catggtggtt gactgtgcgc gcgataaaaa cattccgatc
     5701 cgtattggcg ttaacgccgg atcgctggaa aaagatctgc aagaaaagta tggcgaaccg
     5761 acgccgcagg cgttgctgga atccgccatg cgtcatgttg atcatctcga tcgcctgaac
     5821 ttcgatcagt tcaaagtcag cgtgaaagcg tctgacgtct tcctcgctgt tgagtcttat
     5881 cgtttgctgg caaaacagat cgatcagccg ctgcatctgg ggatcaccga agccggtggt
     5941 gcacgcagcg gggcagtaaa atccgccatt ggtttaggtc tgctgctgtc ggaaggcatc
     6001 ggcgacacgc tgcgtgtatc gctggcggcc gatccggtcg aagagatcaa agtcggtttc
     6061 gatattttga aatcgctgcg tatccgttcg cgagggatta acttcatcgc ctgcccgacc
     6121 tgttcgcgtc aggaatttga tgttatcggt acggttaacg cgctggagca acgcctggaa
     6181 gatatcatca ctccgatgga cgtttcgatt atcggctgcg tggtgaatgg cccaggtgag
     6241 gcgctggttt ctacactcgg cgtcaccggc ggcaacaaga aaagcggcct ctatgaagat
     6301 ggcgtgcgca aagaccgtct ggacaacaac gatatgatcg accagctgga agcacgcatt
     6361 cgtgcgaaag ccagtcagct ggacgaagcg cgtcgaattg acgttcagca ggttggaaaa
     6421 ataataacgt gatgggaagc gcctcgcttc ccgtgtatga ttgaacccgc atggctcccg
     6481 aaacattgag ggaagcgttg agggttcatt tttatattca gaaagagaat aaacgtggca
     6541 aaaaacattc aagccattcg cggcatgaac gattacctgc ctggcgaaac ggccatctgg
     6601 cagcgcattg aaggcacact gaaaaacgtg ctcggcagct acggttacag tgaaatccgc
     6661 ttgccgattg tagagcagac cccgctattc aaacgtgcga ttggtgaagt caccgacgtg
     6721 gttgaaaaag agatgtacac ctttgaggat cgcaatggcg acagcctgac tctgcgccct
     6781 gaagggacgg cgggctgtgt acgcgccggc atcgagcatg gtcttctgta caatcaggaa
     6841 cagcgtctgt ggtatatcgg gccgatgttc cgtcacgagc gtccgcagaa agggcgttat
     6901 cgtcagttcc atcagttggg ctgcgaagtt ttcggtctgc aaggtccgga tatcgacgct
     6961 gaactgatta tgctcactgc ccgctggtgg cgtgcgctgg gtatcttcga acacgtaact
     7021 cttgagctga attctatcgg ttcgctggaa gcacgcgcca attaccgcga tgcgctggtg
     7081 gcattccttg agcagcataa agaaaagctg gacgaagact gcaaacgccg catgtacact
     7141 aacccgctgc gcgtgctgga ttcaaaaaat ccggaagtgc aggcgcttct caacgacgct
     7201 ccggcattag gtgattatct ggacgaggaa tctcgtgagc attttgccgg tctgtgcaaa
     7261 ctgctggaga gcgcggggat cgcttacacc gtaaaccagc gtctggtgcg tggtctggat
     7321 tactataacc gtaccgtttt cgagtgggtg actaacagtc tcggctccca gggcaccgtg
     7381 tgtgcaggcg gtcgttatga cggtcttgtg gaacaactgg gcggtcgtgc aacaccggct
     7441 gtcggttttg cgatgggcct cgaacgtctt gtattgttag tacaggccgt taatccggaa
     7501 tttaaagccg atcctgttgt cgatatatac ctggtggctt caggtgctga tacacaatct
     7561 gcggctatgg cattagctga gcgtctgcgt gatgaattac tgggcgtgaa attgatgacc
     7621 aaccacggcg gcggcaactt taagaaacag tttgcccgtg ctgataaatg gggtgcccgc
     7681 gttgctgtgg tgctgggtga gtctgaagtg gctaacggca cagcagtagt gaaggatttg
     7741 cgctctggtg agcaaacggc agttgcgcag gatagcgtag ccgcgcattt gcgcacgtta
     7801 ctgggttaag gaaggagaag gacagcgtgg aaatttacga gaacgaaaac gaccaggtag
     7861 aagcggttaa acgctttttt gctgaaaatg gcaaagcact ggctgttggg gggattttgg
     7921 gcgttggcgc actgactggc tggcgctact ggaacagcca tcaggttgat tctgcacgct
     7981 ccgcttctct tgcctatcaa aatgcggtta ccgcagtgag cgaaggcaaa ccggatagca
     8041 tcccggcggc ggaaaaattt gctgctgaaa ataaaaatac ttatggtgcg ctggcttctt
     8101 tggaacttgc gcagcaattt gttgacaaaa atgaactaga gaaagctgcc gcccagttac
     8161 aacaggggct ggcagacacg agcgatgaaa atctcaaagc cgtgataaat ctgcgtcttg
     8221 ctcgcgttca ggtacagctc aagcaggctg atgccgcgct gaaaaccctt gataccatca
     8281 aaggtgaagg gtgggctgcc attgttgccg acttgcgtgg tgaagcattg ctgagcaaag
     8341 gtgataagca aggtgcgcgt agtgcatggg aagcaggcgt gaaaagcgat gttactccgg
     8401 cactgagcga aatgatgcag atgaaaatta ataatttgtc catctgagag ggacccgatg
     8461 caattgcgta aattactgct gccaggactg ctttccgtta cccttttaag cggctgttcg
     8521 ctgtttaaca gcgaagaaga tgtggtaaag atgtccccat tgccaaccgt tgaaaaccag
     8581 tttacgccga ccacggcgtg gagcacttcc gttggtagcg gcattggcaa cttctattcc
     8641 aatcttcatc cggcactggc ggacaacgtt gtctatgcag cggaccgcgc tggtttggta
     8701 aaagcgctga atgcggatga tggcaaagaa atctggtctg tcagcctggc cgagaaagat
     8761 ggctggttct ctaaagagcc tgcattactt tctggcggtg tgaccgtgtc tggtgggcat
     8821 gtctacattg gcagcgaaaa ggcgcaggtt tacgcgctga ataccagcga tggtactgtg
     8881 gcatggcaaa ctaaagtcgc gggtgaagca ctttcgcgcc cggtggtcag cgacggtctg
     8941 gtgttaatcc acaccagtaa cggtcagtta caagcgctga acgaagctga cggcgctgtc
     9001 aaatggacag ttaacctcga tatgccttcg ctctctttgc gtggcgagtc tgcgccggca
     9061 acggcttttg gtgcggccgt cgtgggtggc gataatggtc gcgtcagcgc agtgctgatg
     9121 gaacagggcc agatgatttg gcagcagcgt atttcccagg cgaccggttc taccgaaatt
     9181 gaccgtctga gcgatgttga cacgactccc gtcgttgtta acggcgttgt tttcgcgctg
     9241 gcctataatg gtaacctgac ggcgcttgat ctgcgcagtg gtcagattat gtggaaacgc
     9301 gaactgggtt cggtgaatga tttcatcgtc gatggcaatc gcatctatct ggtcgatcaa
     9361 aatgaccggg tgatggcgtt gaccattgat ggcggcgtta cgctgtgggc acaaagcgat
     9421 ctgctgcatc gcctgctgac ttctccggtg ctgtataatg gcaacctggt ggtcggtgac
     9481 agtgaaggtt atctgcactg gattaacgtc gaagatggtc gtttcgttgc ccagcaaaaa
     9541 gttgatagtt ccggtttcca gactgaaccg gttgccgctg acggcaaact gctgatccag
     9601 gcaaaagacg gaaccgtgta ctctattaca cgttaatcgt ctctgtcgtt cactttgaaa
     9661 acggctcctg gacaggggcc gttttcctgt ttttaacaac gacgcgaata tagcgtgcgt
     9721 tgtctgatga tttataaaaa tgaggcttta aacatggtac ctgtggtcgc gcttgtcggg
     9781 cgccctaacg taggaaaatc cacgttattt aaccgtctaa ctcgcacccg agatgcgctg
     9841 gttgcggatt tcccgggtct gactcgtgac cgtaagtacg gtcgtgcgga aattgaaggc
     9901 cgtgagttta tctgtattga taccggcggg attgatggca cagaagacgg tgtagaaacc
     9961 cgcatggcgg aacagtcgct gctggcgatt gaagaagcgg acgtcgtact gtttatggtg
    10021 gatgcgcgcg cgggcctgat gccggcagat gaagcgattg ccaaacatct gcgctcccgt
    10081 gaaaaaccga ccttcctggt ggtaaacaaa actgacggtc tggatcccga tcaggcagtg
    10141 gttgatttct actcgcttgg tttaggtgaa atctacccga tcgccgcgtc tcacggtcgt
    10201 ggcgtattaa gtctgctgga gcatgtgctg ctgccgtgga tggaagatct cgcaccgcaa
    10261 gaggaagtcg acgaagacgc tgaatactgg gcgcaatttg aagcggaaga gaacggcgaa
    10321 gaagaagagg aagacgactt cgacccgcaa agtctgccga tcaaactggc gattgtgggt
    10381 cgtccgaacg taggtaagtc tacactcact aaccgtattc ttggagaaga gcgcgttgtt
    10441 gtttacgaca tgcctggcac gacgcgtgac agcatctaca tcccaatgga acgcgatgga
    10501 cgtgagtatg tgctcattga caccgctggc gtacgtaaac gcggcaaaat caccgatgct
    10561 gtagagaaat tctccgtaat caaaacgttg caggccattg aagacgccaa cgtggtgatg
    10621 ttagtgattg atgcgcgcga aggtatttcc gatcaggatc tctcgctgct gggctttatt
    10681 ctcaatagtg ggcgctcact tgtcattgtg gtgaataagt gggatggcct gagccaggaa
    10741 gtgaaagagc aggtgaaaga aacgctggac ttccgtctgg gctttatcga ttttgctcgt
    10801 gtgcacttta tctctgcctt gcacggcagt ggtgttggta acttgtttga atcagtacgt
    10861 gaagcgtatg acagctccac ccgtcgtgtg gggacctcta tgctgacgcg catcatgacg
    10921 atggctgttg aagatcacca accgccgctg gtacgcggtc gtcgtgtgaa gctgaaatat
    10981 gcccacgctg gtggttataa cccgccgatt gtggtgattc acggtaatca ggtgaaagac
    11041 ctgcctgatt cctacaagcg ctacttgatg aactacttcc gcaaatcgct ggacgtaatg
    11101 ggatcgccga ttcgtattca gttcaaagaa ggggaaaacc cgtatgcgaa caagcgtaac
    11161 accctgacgc caacccagat gcgtaaacgt aagcgtctga tgaagcacat caagaaaaat
    11221 aaataattat ccgtaagtat tttatctgcc attccctcta tgaatgtaga gggaatggct
    11281 accggaggaa ttcatatgga actgcattgt ccaaaatgtc agcatgtgct tgatcaggat
    11341 aacggccatg cgcgttgccc atcctgcggg gaatttattg aaatgaaagc tctttgtcca
    11401 gactgccatc aaccgttgca ggtgttaaaa gcctgcggtg cagtagatta cttctgtcag
    11461 cacgggcatg gcttgatatc gaaaaaacgc gttgaattcg tgctggctta atgaaccttt
    11521 ttacgcgatt tttttaccgg ctggatgttt tttacttcac tttctatcca gccgtcttcc
    11581 agacgtgtgg ttagcatttc acccgtttta acttgcttca cttttttcag tacattgccg
    11641 tcagtagcag tagtaacgct gtatccacgc gccagcgttg acagtgggct tacggcttcg
    11701 aggtgcgtta ctgcattacc gaaacgttca cgcgtggcgc taagctgtgc gcgcaggatt
    11761 tctgctaaac gatattccag ttgctgaatg cgcgtttgcg tgcgatgaat cttcggctgt
    11821 ggattttgct gattcagccg ctgtgttaat cgctgctgct gttgcccggc acgcttaagc
    11881 tgattttcca gcgcaaagct catccgtttt tgcaggcgtt caagcatggt ttgctggcgt
    11941 gccagccgga gctgcggatg ctgctgctgc aagcgatgat gaatctgcgt aaagcgacgt
    12001 gtacggttgg cgagataata atccatcgcc atctccagcc gttgacgggc cgattgcacc
    12061 tggcgcagta actcttgctg attacggctc actacttcag cggcggcaga cggcgttggc
    12121 gcacgcagat cggcaacaaa atcggcaatg gtcacatccg tctcatgccc gacggcgctg
    12181 acgaccggaa tgcggctggc aaaaatcgcc cgcgctacgc gttcgtcgtt aaaactccat
    12241 aaatcttcca gcgaaccgcc gccgcgccca acgatcaaca cgtcgcactc attgcgctga
    12301 ttcgccagtt caatggcgcg aacaatttgc cccggcgcgt catcgccctg aacggaggtg
    12361 gggtagatga tcaccggtag agaaggatcg cgacgtttta acacatgcaa aatatcatgt
    12421 agcgcagcac cggtttttga ggtgatcaca ccaacgcaat gcgcagggga gggaagtggt
    12481 tttttgtatt gctgatcgaa caaacattca gcctgcaact tcgctttgag ctgttcgtac
    12541 ttctgttgca gcagcccttc accggccggc tgcatactct caacgattat ctggtagtcg
    12601 ccgcgcggct cgtagagcgt aatattggcg cgaactaaaa cttgttgccc atgctgcgga
    12661 cggaaggtga cccggcggtt gctgttgcgg aacatcgcgc agcgtacctg ggcggtgtcg
    12721 tctttgagtg taaagtacca gtgaccggaa gccggttgtg tgaaattaga gatttcgccg
    12781 ctgatccaaa cctgtcccat ctcatgctca agcagcagac gaaccgtttg attcaggcga
    12841 ctaacggtaa aaattgcagg ggattgagaa ggtaacatgt gagcgagatc aaattctaaa
    12901 tcagcaggtt attcagtcta tagtaacccg cccttcgggg atagcaagca ttttttgcaa
    12961 aaaggggtag atgcaatcgg ttacgctctg tataatgccg cggcaatatt tattaaccac
    13021 tctggtcgag atattgccca tgctacgtat cgctaaagaa gctctgacgt ttgacgacgt
    13081 tctcctcgtt cctgctcatt ctaccgttct gccgaatact gctgacctca gcacccagct
    13141 gacgaaaact attcgtctga atatccctat gctttccgca gcaatggata ccgtaacgga
    13201 agcgcgcctg gctattgctc tggctcagga aggcggtatc ggctttatcc acaaaaacat
    13261 gtccattgaa cgccaggcag aagaagttcg ccgtgtgaaa aaacacgaat ctggtgtggt
    13321 gactgatccg cagactgttc tgccaaccac gacgctgcgc gaagtgaaag aactgaccga
    13381 gcgtaacggt tttgcgggct atccggtcgt taccgaagaa aacgaactgg tgggtattat
    13441 caccggtcgt gacgtgcgtt ttgttaccga cctgaaccag ccggttagcg tttacatgac
    13501 gccgaaagag cgtctggtca ccgtgcgtga aggtgaagcc cgtgaagtgg tgctggcaaa
    13561 aatgcacgaa aaacgcgttg aaaaagcgct ggtggttgat gacgaattcc acctgatcgg
    13621 catgatcacc gtgaaagact tccagaaagc ggaacgtaaa ccgaacgctt gtaaagacga
    13681 gcaaggccgt ctgcgtgttg gtgctgcagt tggcgcaggt gcgggtaacg aagagcgtgt
    13741 tgacgcgctg gttgccgctg gcgttgacgt tctgctgatc gactcctccc acggtcactc
    13801 agaaggcgtt ttgcagcgta tccgtgaaac ccgtgctaaa tatccggatc tgcaaatcat
    13861 cggcggcaac gtggcaacag ctgcaggtgc acgcgctttg gcagaagctg gttgcagcgc
    13921 ggttaaagtc ggtatcggcc ctggttctat ctgtacgact cgtatcgtta ctggcgtagg
    13981 tgttccgcag atcactgccg ttgctgacgc agttgaagcc ctggaaggca ccggtattcc
    14041 ggttatcgca gatggtggta ttcgcttctc cggcgacatc gccaaagcta tcgccgctgg
    14101 cgcaagcgcg gtgatggtag gttccatgct ggcgggtact gaagaatctc cgggtgaaat
    14161 cgaactctac cagggccgtt cttataaatc ttaccgtggt atgggttccc tgggcgcgat
    14221 gtccaaaggt tcctctgacc gttatttcca gagcgataac gctgccgaca aactggtgcc
    14281 ggaaggtatc gaaggtcgcg tagcttataa aggtcgcctg aaagagatca ttcaccagca
    14341 gatgggcggc ctgcgctcct gtatgggtct gaccggctgt ggtactatcg acgaactgcg
    14401 tactaaagcg gagtttgtac gtatcagcgg tgcgggcatt caggaaagcc acgttcacga
    14461 cgtgaccatt actaaagagt ccccgaacta ccgtctgggc tcctgattct cttcgcccga
    14521 tttcatgtcg ggcgatttat attatctgtt tcacttgcct cggaataagc gtcaatgacg
    14581 gaaaacattc ataagcatcg catcctcatt ctggacttcg gttctcagta cactcaactg
    14641 gttgcgcgcc gcgtgcgtga gctgggtgtt tactgcgaac tgtgggcgtg ggatgtgaca
    14701 gaagcacaaa ttcgtgactt caatccaagc ggaattattc tttccggcgg cccggaaagt
    14761 actactgaag aaaacagtcc gcgtgcgccg cagtatgtct ttgaagcagg cgtaccgata
    14821 ttcggcgttt gctatggcat gcagaccatg gcaatgcagt tgggcggtca cgttgaagcc
    14881 tctaacgaac gtgaatttgg ctacgcgcag gtagaagtcg taaacgacag cgcattggtt
    14941 cgcggtatcg aagatgcgct gaccgcagac ggtaaaccgc tgctcgatgt ctggatgagc
    15001 cacggcgata aagttaccgc tatcccgtcc gacttcgtca ccgtagccag caccgaaagc
    15061 tgcccgtttg ccattatggc taacgaagaa aaacgcttct atggcgtaca gttccacccg
    15121 gaagtgactc acacccgcca gggtatgcgc atgctggagc gttttgtgcg tgatatctgc
    15181 cagtgtgaag ccctgtggac gccagcgaaa attatcgacg atgctgtagc tcgcatccgc
    15241 gagcaggtag gcgacgataa agtcatcctc ggcctctctg gtggtgtgga ttcctccgta
    15301 accgcaatgc tgctgcaccg tgctatcggt aaaaacctga cttgcgtatt cgtcgacaac
    15361 ggcctgctgc gtctcaacga agcagagcag gttctggata tgtttggcga tcactttggt
    15421 cttaacattg ttcacgtacc ggcagaagat cgcttcctgt cagcgctggc tggcgaaaac
    15481 gatccggaag caaaacgtaa aatcatcggg cgcgttttcg ttgaagtatt cgatgaagaa
    15541 gcgctgaaac tggaagacgt gaagtggctg gcgcagggca ccatctaccc tgacgttatc
    15601 gaatctgcgg cttctgcaac cggtaaagca cacgtcatca aatctcacca caacgtgggc
    15661 ggcctgccga aagagatgaa gatgggcctg gttgaaccgc tgaaagagct gttcaaagac
    15721 gaagtgcgta agattggtct ggagctgggc ctgccgtacg acatgctgta ccgtcatccg
    15781 ttcccgggac caggccttgg cgttcgtgtt ctgggtgaag tgaagaaaga gtactgtgac
    15841 ctgctgcgcc gtgctgacgc catcttcatt gaagaactgc gtaaagcgga cctgtacgac
    15901 aaagtcagcc aggcgttcac cgtcttcctg ccagtacgtt ccgttggcgt aatgggcgat
    15961 ggtcgtaagt atgactgggt tgtctctctg cgtgctgtcg aaaccatcga ctttatgacc
    16021 gcacactggg cgcatctgcc gtacgatttc ctcggtcgcg tttccaaccg cattatcaat
    16081 gaagtgaacg gtatttcccg cgtggtgtat gacatcagcg gcaagccgcc agctaccatt
    16141 gagtgggaat gattttgatc cctgcactat gaatgaacaa aaccctccgt tactacagag
    16201 ggttttttat cttcaagaat cataggcttg aagttactaa catcgattaa ttaaaccagc
    16261 tgtccgattt gttctcttct gctttgccca cgcttttcat cagatcgcga ccgccttcag
    16321 tcatatttct gtttgcgtca gcttcagatt gcaccacatc ggtttgcgca gctttgtgct
    16381 tcagttcctg atcgataaat tcgttttcgc gcttaacgcg ggcttcttct ttcgccagcg
    16441 ccagtttttg tttctgaatc tctaagctgc gtagctcatc ttcataactt tgatcgcgtt
    16501 ttttgtccgc agtggcttcg gcgtccagtt tatcctgacg agctttctta tttgccgctg
    16561 ccgttgccgc tcttttgttc gcggcggcct gggcatttgc gcgacgttgc ttctcttgct
    16621 ggatttccct gttgcgctcc gcgacccatt cgtcatgctg cctttgctct tcatttttac
    16681 cttgctgttc cgcttctgcg acagcagaga gttgatcctg caatgatgag gctatagccg
    16741 gatagcttaa ggaggctaag atggcgcaaa gaaaaacttt cttcatgact cctcctgatt
    16801 attagctctt ttcaggacat ttggtatttg gctgaatacg cgtttcgtta tacgttgtgg
    16861 taataacaac ggctaaacct gtcgtaaact ggcactcttt accctcctgg gtggaggtat
    16921 acactttggt gccttcctta tatgttaaag aaacaccttc cactaaggtt ttatcattca
    16981 ccatagaacc cgctgccgcg cctacagctc cgccgccaac tgcccctgcc gtcgttccgg
    17041 aattgctgcc agaaccgacg ttgtggccga taacaccgcc agcgactgcg ccaataagcg
    17101 cgccgaaggc ttgtgcgttc cgtttatttt gggagttgtc tacggcaact tttgcgggaa
    17161 gaatggaaat aatattaacg gttttagttt cttgtttggt attcagttga tcggtttgat
    17221 aaacatcggc ggcatgatcg tcagcatttg actggcatcc tgccagagtg aatgacgcta
    17281 acattgccac aggcagaaga cattttttaa atttcatcac tattccttgt tatacacatt
    17341 acgaaaaata agacatttaa gatcatcaaa agaggaaagt aaatgtattg cgtataaagg
    17401 gatagattaa atttattaaa attcacgcag ggaataattt ataaaaatat atcattacaa
    17461 catatatatt aattttatat ttatttcgca gccaaacagg ataaacatca gtcaggacaa
    17521 agacgtaaaa tttaagatga aagaatatct gccgctttga taatcccaaa gagcaaaagc
    17581 atccgacagc gaagtgccgt tatgttctgt gaaagtggag aaggtaaccg ctaacgttgt
    17641 accggtgatt cgacggagag tgtggattgc tgagcttctt ttttgctctg atggtgctgc
    17701 caggcaccga cggacgagta gataaaacgg ccaaagaaga agataaagct gatgagcagt
    17761 acgatacggg tcatgcgact gttaaatcgg tgtcgttttc gcatactagt tgcctgactc
    17821 acaaaaggtt ccttgaagta tgtcccacgc tgtggacggt acttacatta aggcacaaca
    17881 ggacaaaatg gtcaattctt cgttatgtaa aaaagcgtca gtggatacat attttaatgt
    17941 tatggaagtt aatttaatta tttacaatga tgatgtaata atgatgaatt aacatgataa
    18001 acgtaataat tcattaatca gggttatttt gtttgatata tatcaattgg aactttcata
    18061 tgacacttag aatcaatgct ctctatagtg atgaataatc atcattcgaa gtcaggtggg
    18121 atgcctgtct gaatacacct ccttcaggat gtgggggatt ctgctgagca tctatgaaac
    18181 tgaatgcaac ttatataaaa atacgtgata aatggtgggg gcttccgctg ttcctgcctt
    18241 ctttaatctt gcccattttc gcccacatta atactttcgc gcatatttct tccggtgagg
    18301 tttttctctt ttatctgcct ttggcactga tgatcagcat gatgatgttt ttcagctggg
    18361 cggcattgcc agggatcacc ttagggattt ttgtccgcaa atatgcagag ctgggttttt
    18421 acgaaacgct ctcattaacg gctaatttta ttatcattat cattctctgt tggggcggtt
    18481 acagggtctt tactccccgg cgtaacaacg tttcacatgg tgatacccgt ttaatttccc
    18541 agcgtatatt ctggcagata gtgtttcctg caacgctgtt tctgatactt ttccagtttg
    18601 ctgcatttgt aggattactg gcgagcagag aaaatctggt cggtgtcatg ccttttaacc
    18661 tcgggacttt aatcaattat caggccttac tggtgggtaa tctgatcggt gtcccgctgt
    18721 gctacttcat cattcgggta gtgcgaaatc cattttattt acgtagctat tattcgcaat
    18781 taaaacagca ggttgatgcc aaagtcacca aaaaagagtt cgcgctctgg ctactggcat
    18841 taggtgcttt attgttgctg ttatgcatgc cgttaaatga aaaaagcaca atttttagca
    18901 ccaattatac cttgtcatta ttgctgcccc tgatgatgtg gggagcaatg cgctatggtt
    18961 ataagctgat ttcgctgctc tgggcggtcg tgttgatgat cagcatccac agctatcaaa
    19021 attacattcc catttatcct ggctatacca cgcagctgac cataacctcc tccagttatc
    19081 tggtattctc ttttattgtc aattatatgg ctgtactggc aactcgtcag cgagcggtag
    19141 tcagacgcat tcagcggctt gcgtatgtgg acccggtggt tcatctgcca aatgttcgcg
    19201 ccctgaatcg cgcgttgcgt gatgccccct ggtctgcgct ttgttattta cgcatccctg
    19261 gcatggaaat gctggttaag aactatggca tcatgctgcg gattcaatat gaggtgtact
    19321 ggcaatagcg gacact
//
LOCUS       DAJTSI010000076        18946 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000076 DAJTSI010000000
VERSION     DAJTSI010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18946
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.076"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1418
                     /gene="kdpA"
                     /locus_tag="OO327_002376"
     CDS             <1..1418
                     /gene="kdpA"
                     /locus_tag="OO327_002376"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCR6018895.1"
                     /translation="LPLNPQQLPGLSWDLALNTAVSFVTNTNWQSYSGETTLSYFSQM
                     AGLTVQNFLSAASGIAVIFAFIRAFTRQSMSTLGNAWVDLLRITLWVLVPVALLIALF
                     FIQQGAQQNFLPYQAVNTVEGAQQLLPMGPVASQEAIKMLGTNGGGFFNANSSHPFEN
                     PTALTNFVQMLAIFLIPTALCFAFGEVTGDRRQGRMLLWAMSVIFVICVGVVMWAEVQ
                     GNPHLLALGADSSINMEGKESRFGVLVSSLFAVVTTAASCGAVIAMHDSFTALGGMVP
                     MWLMQIGEVVFGGVGSGLYGMMLFVLLAVFIAGLMIGRTPEYLGKKIDVREMKLTALA
                     ILVTPTLVLMGAALAMMTDAGRSAMLNPSPHGFSEVLYAVSSAANNNGSAFAGLSANS
                     PFWNCLLAFCMFVGRFGVIIPVMAIAGSLVSKKSQPASSGTLPTHGPLFVGLLIGTVL
                     LVGALTFIPALALGPVAEYLS"
     gene            1441..3488
                     /gene="kdpB"
                     /locus_tag="OO327_002377"
                     /pseudo
     CDS             1441..3488
                     /gene="kdpB"
                     /locus_tag="OO327_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            3497..4069
                     /gene="kdpC"
                     /locus_tag="OO327_002378"
     CDS             3497..4069
                     /gene="kdpC"
                     /locus_tag="OO327_002378"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCR6018896.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4062..6742
                     /gene="kdpD"
                     /locus_tag="OO327_002379"
                     /pseudo
     CDS             4062..6742
                     /gene="kdpD"
                     /locus_tag="OO327_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            6739..7424
                     /gene="kdpE"
                     /locus_tag="OO327_002380"
                     /pseudo
     CDS             6739..7424
                     /gene="kdpE"
                     /locus_tag="OO327_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
     gene            7699..7803
                     /locus_tag="OO327_002381"
     CDS             7699..7803
                     /locus_tag="OO327_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCR6018897.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            8114..10311
                     /gene="speF"
                     /locus_tag="OO327_002382"
                     /pseudo
     CDS             8114..10311
                     /gene="speF"
                     /locus_tag="OO327_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            10308..11627
                     /gene="potE"
                     /locus_tag="OO327_002383"
     CDS             10308..11627
                     /gene="potE"
                     /locus_tag="OO327_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR6018898.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(11683..13323)
                     /gene="pgm"
                     /locus_tag="OO327_002384"
     CDS             complement(11683..13323)
                     /gene="pgm"
                     /locus_tag="OO327_002384"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR6018899.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDKANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(13349..13894)
                     /gene="seqA"
                     /locus_tag="OO327_002385"
     CDS             complement(13349..13894)
                     /gene="seqA"
                     /locus_tag="OO327_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR6018900.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            14079..14843
                     /gene="ybfF"
                     /locus_tag="OO327_002386"
     CDS             14079..14843
                     /gene="ybfF"
                     /locus_tag="OO327_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6018901.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            14983..15276
                     /gene="ybfE"
                     /locus_tag="OO327_002387"
     CDS             14983..15276
                     /gene="ybfE"
                     /locus_tag="OO327_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR6018902.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            15416..15946
                     /gene="fldA"
                     /locus_tag="OO327_002388"
     CDS             15416..15946
                     /gene="fldA"
                     /locus_tag="OO327_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR6018903.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            16156..16242
                     /gene="uof"
                     /locus_tag="OO327_002389"
     CDS             16156..16242
                     /gene="uof"
                     /locus_tag="OO327_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR6018904.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            16235..16681
                     /gene="fur"
                     /locus_tag="OO327_002390"
     CDS             16235..16681
                     /gene="fur"
                     /locus_tag="OO327_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR6018905.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(16765..17091)
                     /locus_tag="OO327_002391"
     CDS             complement(16765..17091)
                     /locus_tag="OO327_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018906.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(17128..18534)
                     /gene="chiP"
                     /locus_tag="OO327_002392"
     CDS             complement(17128..18534)
                     /gene="chiP"
                     /locus_tag="OO327_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCR6018907.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         4369 a         4779 c         5067 g         4731 t
ORIGIN      
        1 atctgccgct taatccacag cagttgccgg ggctgtcgtg ggatctggcg ctgaataccg
       61 ccgtcagctt tgtcacgaat accaactggc aatcttatag cggtgaaacc acgttgagct
      121 atttcagcca gatggcaggc ttaacggtac aaaactttct ttctgccgcc agcgggattg
      181 cggtgatttt tgccttcatc cgtgcgttta ctcgccagag catgagcaca ctcgggaatg
      241 cctgggtcga tctgctacgc atcacgttat gggtgctagt ccctgtggcg ttgttgatcg
      301 cactgttttt tattcaacaa ggtgcgcagc aaaactttct gccctatcag gcagtgaata
      361 ccgttgaagg agcgcaacag ctgttaccca tgggaccagt agcttctcag gaagcgatca
      421 agatgctcgg tactaacggc ggtggcttct ttaatgccaa ctcgtcgcat ccgtttgaaa
      481 acccaaccgc actgaccaac ttcgtgcaga tgctggcgat cttcttgatc ccaacagcgc
      541 tgtgctttgc ctttggtgaa gtgacgggcg atcgccgcca ggggcgcatg ttgctgtggg
      601 cgatgtcagt gatttttgtc atctgcgtag gcgtggttat gtgggcagaa gttcagggta
      661 atcctcatct gctggcgctc ggcgcagaca gcagcatcaa tatggaaggt aaagagagcc
      721 gtttcggcgt gctggtcagt agcctgtttg cggtcgtgac tacggcggcg tcctgtggcg
      781 cggtgattgc gatgcatgat tcgtttaccg ctctcggcgg catggtgccg atgtggctaa
      841 tgcaaattgg tgaagtggtg ttcggcggtg tcggttctgg tctttacggc atgatgctgt
      901 ttgtcctgct ggcggtgttt attgccgggc tgatgattgg tcgtacaccg gaatatctgg
      961 gtaaaaaaat agacgtacgc gagatgaaac tgactgcact ggcgattctg gttactccaa
     1021 cactggtact gatgggcgcg gcgttggcga tgatgaccga cgccggacgt agcgccatgc
     1081 ttaaccctag cccgcatggt tttagcgaag tgctgtacgc cgtgtcgtcc gccgctaaca
     1141 acaacggcag cgcctttgcc ggattaagcg ccaactctcc gttctggaac tgtttactgg
     1201 cgttctgcat gtttgtcggt cgcttcgggg tgattatccc ggtgatggca attgccggtt
     1261 cgctggtgag taaaaagagc caacccgcca gctccggcac gctgccaacg cacggcccgc
     1321 tgtttgttgg cctgttaatc ggcaccgtgt tgctggttgg cgcactgacc tttatccctg
     1381 ccctggcgct tggtccggtg gcggaatatc tctcctgatg atattgagtg agcactgaat
     1441 atgagtcgta aacaactggc gctattcgaa ccaacacttg tcgttcaggc gctgaaagaa
     1501 gcggtgaaaa aattaaaccc gcaggcgcaa tggcgcaatc cggtgatgtt tatcgtctgg
     1561 atcggcagtc tgctgaccac ctgtattagc atcgcgatgg caagcggtgc gatgcctggc
     1621 aatgcgctgt ttagcgcggc aattagcggc tggctgtggg tcaccgtact gttcgctaat
     1681 ttcgccgagg cgctggcaga aggccgcagt aaagcgcagg ccaacagtct taaaggggtg
     1741 aaaaaaactg cctttgcccg caagctgcgt gagccgaaat atggcgctgc ggcggacaaa
     1801 gttcctgccg atcaactgcg taaaggtgac atcgtgctgg tagaagctgg cgatattatc
     1861 ccctgcgatg gtgaagtcat tgaaggcggc gcatcggtcg atgaaagcgc catcaccggg
     1921 gaatcggcac cggtgatccg tgaatccggc ggcgattttg cctccgttac cggcggcacg
     1981 cgtattcttt ctgactggct ggtgattgag tgtagcgtta accccggtga gacatttctg
     2041 gatcggatga tcgcgatggt ggaaggcgca cagcgacgca aaacaccgaa cgagattgcc
     2101 ctgactatcc tgctgattgc tctgaccatc gtctttttac tggcaaccgc cacgctgtgg
     2161 ccgttttccg cgtggggcgg taatgccgtg agtgtaacgg tactggtggc gctgctggtc
     2221 tgtctgatcc caaccactat tggtggcctg ttgtcagcga tcggcgtcgc cgggatgagt
     2281 cggatgctgg gcgcgaatgt gattgccacc agcggacgtg cagttgaagc ggcaggtgac
     2341 gttgacgttc tgctactgga taaaaccggc accatcacac tcggtaaccg tcaggcgtcg
     2401 gagtttatcc ccgcgcaggg cgtggatgaa aaaacgctgg ctgacgccgc acaactggct
     2461 tcgctggctg atgaaacgcc ggaaggtcgc agtattgtga tcctcgccaa gcaacgtttt
     2521 aacctgcgcg agcgcgatgt gcagtcgctc catgccacct ttgtaccgtt tactgcgcaa
     2581 agccggatga gcgggatcaa catcgacaac cgcatgatcc gtaaaggttc tgtcgatgcc
     2641 attcgccgcc atattgaggc taacggcggt cacttccctg ccgatgttga tcaaaaagtc
     2701 gatcaggttg cgcgtcaagg agccacgctg ctggtggtgg tggaaggttc tcgtgtgctg
     2761 ggcgttattg cgctgaaaga tatcgtcaaa ggcggtatta aagagcgctt cgcccagctg
     2821 cgcaaatggg cattaaaacg gtgatgatta ccggcgataa ccgtctgact gccgccgcga
     2881 tagctgcgga agcgggtgtc gatgattttc tcgccgaagc gacaccggag gccaagctgg
     2941 cattgatccg tcagtatcag gcggaaggtc gtttggtagc gatgaccggc gacagcacca
     3001 acgatgctac ggcgctggcg caggcagatg tcgcggtggc gatgaactcc ggcacccagg
     3061 cggctaaaga ggcgggcaat atggtggatc tcgactctaa cccgaccaag ttgatcgagg
     3121 tggtgcacat tggcaaacag atgctgatga cccgtggctc gctgaccacc ttcagcattg
     3181 ccaacgatgt ggcgaaatac ttcgccatta ttccggcggc gttcgcggca acatatccgc
     3241 agttaaatgc gctgaacatc atgcgcctgc attcgcccga ctccgccatt ctcagtgcgg
     3301 tcattttcaa cgccttgatt atcgtctttt tgattcctct ggcgttaaaa ggcgtgagtt
     3361 ataaaccgct taccgcttct gccatgttgc gccgtaactt atggatttac ggtctgggtg
     3421 ggctgctggt gccgtttatt ggtatcaaag tcattgattt actgctaacc gtttgtagtc
     3481 tggtgtgagg tttaccatga gtggattacg tccggcatta tcgacatttc tctttctgtt
     3541 attgattact ggcggcgttt acccgctgct gaccaccgca ctggggcaat ggtggtatcc
     3601 ctggcaggcc aacggttcgt tgattcgtga aggtgatacg gtgcgcggtt cggcattaat
     3661 cgggcaaaat tttaccggca acggctattt tcatggtcgc ccgtcggcaa cagcagaaat
     3721 gccctataat ccacaggctt ctggcgggag caatctggca gtcagtaacc cggagctgga
     3781 taaacagata gccgcacgcg ttgccgcatt acgggccgct aacccggacg ccagcacgaa
     3841 cgttccggtt gaactggtga cggcgtcggc aagcggactg gacaataaca tcaccccgca
     3901 agcggcggcc tggcagatcc cacgcatcgc aaaagcgcgt aatctcagcg ttgaacagct
     3961 cacgcagctg atcgcaaaat acagccaaca accgctggtg aaatatatcg gccagccggt
     4021 cgtcaacatt gtcgaactca atctggcact ggataagctt gatgaataac gaacccttac
     4081 gtcccgaccc cgaccgtctg ctggaacaaa ctgccgcgcc gcatcggggg aagctgaaag
     4141 ttttcttcag tgcctgtgca ggcgtcggga agacctgggc gatgctggca gaagcccagc
     4201 gactgcgggc gcaagggctg gatattgtgg ttggtgtggt agaaacccat gggcggaaag
     4261 ataccgccgc catgcttgaa gggctggctg ttctgcctct gaaacgccag gcgtaccgtg
     4321 ggcggcatat cagcgagttt gatctcgatg ccgccctcgc ccgccgtccg gcgctgatct
     4381 taatggacga actggcgcac agtaatgcgc caggttctcg tcatcctaaa cgctggcagg
     4441 atatcgaaga actgctggaa gctggcattg atgttttcac aaccgtcaac gttcagcatc
     4501 tggaaagtct gaatgatgtg gtcagcggcg tcaccggaat tcaggtacgg gaaaccgtgc
     4561 ccgatccttt tttcgatgcc gccgacgacg tggtgctggt ggacttgccc ccggacgatc
     4621 tgcgccagcg gctgaaagaa ggcaaagtct atattgccgg gcaggcggag cgcgccattg
     4681 aacatttttt ccgcaaaggc aatctgatcg ccctgcgcga actggcactg cgccgtactg
     4741 ccgatcgcgt tgatgagcaa atgcgcgcct ggcgggggca tcctggcgaa gagaaagtct
     4801 ggcacacgcg cgacgcgatc cttttatgca tcggccataa caccggcagc gaaaactggt
     4861 ccgcgcggcg gcgcggctga cgtcacggct ggggagcgtc tggcacgcgg tgtatgttga
     4921 aacccctgcc ctgcatcgct taccggagaa aaaacgtcgg gcaattctca gcgccttacg
     4981 tctggcgcag gagctgggcg cggagacggc aacactttct gatccagcgg aagagaaagc
     5041 ggtagtgcgt tatgcccgtg aacataatct cggcaagatt attctcggtc gcccagcctc
     5101 gcgccgctgg tggcgtcggg aaacatttgc tgaccgactg gcgcgcatcg cccccgatct
     5161 cgatcaggtg ctggtcgcgc ttgatgaacc acccgcccgc acgattaaca acgcgccgga
     5221 tagccgctca tttaaagaca agtggcgggt acagattcag ggatgcgtgg ttgccgccgc
     5281 gttatgcgcc gttatcacct taattgccat gcagtggctg atggcgtttg atgccgccaa
     5341 cctggtgatg ctgtatctgc ttggcgtggt ggtggcgcta ttttatggac gctggccttc
     5401 ggtggttgcc accgtcatta atgtggtgag tttcgatctc ttttttatcg ccccacgcgg
     5461 cacgctcgcc gtctctgatg tgcaatatct gctgaccttc gcggtgatgt taaccgtcgg
     5521 gctggtgatc gggaacctta ctgctggtgt gcgttatcag gcgcgggtag cccgttaccg
     5581 cgagcaacgc acacggcact tatatgaaat gtcgaaagct ctggcggtgg gccgcagtcc
     5641 gcaggacatc gccgccacca gcgaacaatt tattgcctcc acgtttcatg cccgcagtca
     5701 ggtgttgttg cccgatgaca acggtaaatt gcagccgtta acacatccgc aaggaattac
     5761 gccatgggac gatgccatcg cgcagtggag ttttgataaa ggcctgcctg cgggcgcggg
     5821 caccgacacg ttacccggtg taccgtacca gattttgccg ctaaaaagcg gcgagaaaac
     5881 ctacgggctg gtggtggtgg agccggggaa tctgcgccag ttgatgatcc cggaacagca
     5941 gcgcctgctg gagactttta cgctgttagt cgccaatgcc cttgagcggc tgacgctaac
     6001 cgccagcgaa gaacaggcgc ggatggcaag cgaacgtgaa cagatccgca acgccctgct
     6061 ggcagcgctt tcgcatgatt tacgcacgcc gcttacggtg ctgtttggtc aggcagaaat
     6121 cttaacgctc gatctggcaa gcgaaggatc accccacgcc cgccaggcca gcgagattcg
     6181 tcagcatgtg ctgaacacta cccgactggt gaataatcta ctggatatgg cgcgaattca
     6241 gtccggcggc tttaatttga agaaagagtg gttaacgctg gaagaagtag tcggcagcgc
     6301 gctgcaaatg ccggaaccgg gtttatcgtc gcccatcaat ctttctctgc cagaaccgct
     6361 gaccttaatc cacgttgacg ggccactctt tgaacgggtg ctgattaatc tgctggagaa
     6421 cgcggtgaaa tatgcgggtg cgcaggccga aattggtatc gatgcccacg ttgagggcga
     6481 aaatctacaa ctggatgtct gggataacgg ccccggtctt ccgccaggcc aggagcagac
     6541 gatatttgat aagtttgctc gcgggaataa agagtcggcg gtgccgggtg tcgggcttgg
     6601 gttggcaatt tgtcgggcga ttgtggatgt acacggcggc accattaccg cgttcaaccg
     6661 accggaaggt ggtgcctgtt ttcgtgttac acttccccag caaaccgctc ctgaacttga
     6721 agactttcat gaggatatgt gacaaacgtt ctgattgttg aagatgaaca ggctattcgt
     6781 cgctttctgc gcacggcgct ggagggcgac gggatgcgcg tctttgaggc cgaaactctg
     6841 caacgcggct tgctggaagc ggcaacccgt aagccagatt tgattattct cgatctcggc
     6901 ctgcccgatg gtgatgggat tgagtttatc cgcgacctgc gccagtggag cccggtgccg
     6961 gtgattgtgc tttccgcacg cagcgaagag agcgacaaga tcgccgcgct ggatgccgga
     7021 gcggatgatt atctgagtaa gccgtttggt attggcgaat tgcaggcccg tctgcgcgtc
     7081 gctttacgcc gccactccgc cactccgcca ccgccgcgcc cgatccgctg gtgaaatttt
     7141 ccggtgttac cgtcgattta gccgcccgcg tgattcaccg gggtgaggaa gaggtgcatc
     7201 tcacaccaat tgagttccgc ctgctggcgg tgctgctcaa caatgccgga aaagtactca
     7261 cccagcgcca gctccttaac caggtgtggg ggccaaacgc ggtcgaacac agtcactatt
     7321 tgcgtattta tatgggacat ctgcgacaaa aactggaaca ggatccggcc cggcctcgcc
     7381 acttcattac tgaaaccggt attgggtatc ggtttatgct ttgaattgtt attcaataaa
     7441 ttcgcttaaa tagtgattaa tttattatta aacctgcgcc gaatattatt atttggcagg
     7501 tttaatttct taacgcaaat ataaaacacg aaattacaac atttaagtaa caaaacagca
     7561 tctaatatgg cgatattcat aattaatgat ttattatctg gggtatgatc gttttttgtt
     7621 gatcttcttc acagattata gccatttcat ggatagaata actctacctt caactgacac
     7681 agcaagcggt aaaggtaaat ggaaaataat aaccgcttaa tgcctcatat aaggcggaca
     7741 acccatatca tgaagtttgc ccatcgtaat agttttgact ttcatttctt taatgcccgc
     7801 tagtcttctg actaaagggc accccaacgt acaggtctcc ctgactttaa gcattacagg
     7861 ttaatacctg tattcctcgg tgctcatata ctgctaaccc ttttaaactc taaataattc
     7921 gagtcgcagc acttgcaact tgaggtatga cgagtatagc cagttaccgg gctggtctgg
     7981 gttattgcat ctgcaaaaag caaactactg atttatttat cagcggtgga gctttgcttt
     8041 ttttccgacg tgatcgattt ctcctttgag aaattgagga cctgctatta cctgaaataa
     8101 agagatgaat aaaatgtcag aattaaaaat tgccgttagc cgttcttgcc cggattgttt
     8161 ttccactcat cgtgcatgcg tgaatattga cgaaagtaat tatattgacg ttgccgccat
     8221 tattttatca gtcaatgatg ttgaacgtgg aaaactcgat aaaatagacg ctactggcta
     8281 tggcattcct gtttttattg caacggaaaa tgaagaacgt gtaccagcag aatatctgcc
     8341 acgtatttct ggtgtcttcg agcattgcga atcacggaaa gagttttatg gacgtcagtt
     8401 agaaaccgct gctagccatt atgaaacgca actgcgcccg cccttcttcc gcgcactggt
     8461 cgattatgtc aatcagggca atagcgcttt tgactgcccg ggtcatcagg gaggtgagtt
     8521 tttccgtcgt catccggcag ggaatcagtt cgtggagtac ttcggagaaa cgctgttccg
     8581 ttccgactta tgcaacgcag acgtagcaat gggcgatctg ctgattcacg aaggcgcgcc
     8641 atgcattgca cagcaacatg cggcaaaagt gtttaatgcc gataaaacat acttcgttct
     8701 aaatggcact tcatcttcta acaaagtggt tttaaacgcc ctgctaacac cgggtgatct
     8761 ggtgctgttt gatcgcaata accacaaatc taaccaccac ggagcgttgc tacaggctgg
     8821 tgcaacaccg gtttatctgg aaacggcacg taacccgtat ggctttatcg gtggcattga
     8881 tgcgcactgt tttgaagaaa gttacctgcg tgagctgatc gcggaagtcg caccgcagcg
     8941 ggcaaaagag gctcgtcctt tccgcctcgc tgtgattcag ttaggcacct acgacggtac
     9001 gatttataac gcccgccagg tggtggataa aattggtcat ctgtgtgact acattctgtt
     9061 tgactccgca tgggttggct atgagcaatt tatcccgatg atggctgact gttcgccgct
     9121 gttgctggat cttaatgaga acgatccggg tattctagtt acgcaatccg tacataaaca
     9181 gcaggctggt ttttctcaaa cttcacaaat ccataaaaaa gacagccaca tcaaagggca
     9241 acagcgttat gtaccgcaca aacgcatgaa caacgccttt atgatgcacg cctccaccag
     9301 cccgttctat ccgctgtttg ccgcactgga tatcaacgcc aaaatgcatg aaggtgtcag
     9361 cggtcgtaat atgtggatgg attgtgtggt aaatggcatt aatgcccgca aactgatcct
     9421 cgataactgt cagcatattc gtccgttcgt acctgaactg gtggatggta aaccctggca
     9481 gtcgtatgaa acagcgcaaa ttgcggttga tctgcgcttc ttccagtttg taccagggga
     9541 acactggcat tcttttgaag gctatgcaga gaatcaatac tttgtcgatc cttgcaaact
     9601 gttgctgaca accccaggta ttgatgcacg taacggcgaa tatgaagcgt tcggtgtacc
     9661 cgcgacgatt cttgctaact tcctgcgcga aaatggcgta gtgccggaaa aatgcgatct
     9721 taacgccatc ctcttcctgc tgactccggc agaagatatg gccaaacttc agcaacttgt
     9781 gccctgctgg tacgcttcga aaaactgctt gagtccgacg cgccattagc agaagtgctg
     9841 ccttccatct acaaacagca tgaagagcgc tacgccggtt ataccctgcg tcagttgtgc
     9901 caggaaatgc atgatttgta tgcccgccac aacgtgaaac aactgcaaaa agagatgttc
     9961 cgcaaagcgc acttcccaca cgtcagcatg aatccgcaag aagccaacta cgcctattta
    10021 cgcggtgaag tggagctggt tcgtctaccg gatgccgaag gtcgtatcgc tgccgaaggg
    10081 gcgcttcctt atcctccggg tgtgctgtgt gttgttccgg gtgaaatctg gggtggtgct
    10141 gttttgcgtt acttcagcgc tctggaagaa gggatcaacc tgctgccagg ttttgcaccg
    10201 gagctgcagg gtgtctatat cgaagaacat gatggtcgta agcaagtttg gtgctatgtc
    10261 atcaagcctc gtgatgcgca aagcaccctg ttgaaagggg aaaaattatg agtcaggcta
    10321 aatcgaacaa aatgggcgtc gttcagttaa ccatactgac gatggtcaac atgatgggct
    10381 ccggtatcat catgctgccg acaaagcttg ccgaagttgg gacaatttca attatctcct
    10441 ggctggtgac agccgtaggc tcaatggcac tggcatgggc attcgcaaaa tgcggtatgt
    10501 tcagccgtaa atcaggtggt atgggcggtt atgccgagta tgctttcggt aaatccggta
    10561 actttattgc gaactatacc tacggcgtct cactgctgat tgctaacgtc gcgattgcta
    10621 tttcggcggt tggttacggc accgaactgc tcggcgcgag tttgtcgcca gtgcagattg
    10681 gtcttgcgac catcggggtg ctgtggattt gtaccgtggc taactttggt ggtgcgcgca
    10741 ttaccgggca aatcagtagc attaccgtgt gggggggcat tattccggtg gtaggcctgt
    10801 gcattatcgg ctggttctgg tttagcccga cgctttacgt tgattcctgg aatccgcatc
    10861 atgcaccgtt cttcagtgca gtaggttctt ccatcgctat gacgctgtgg gcttttcttg
    10921 gtctggagtc tgcgtgtgcg aatactgatg tagtggaaaa cccggaacgt aatgtgccaa
    10981 tcgcggtact cggcggtacg ttaggtgcgg cggtgattta tatcgtctcc accaacgtga
    11041 ttgcagggat tgtgcctaat atggagctgg caaattcaac ggcaccattt ggcctggcct
    11101 tcgcacagat gtttacacca gaagtgggta aagtcattat ggcgctgatg gtgatgtcct
    11161 gctgcggttc gctgcttggc tggcagttca ccattgccca ggtgtttaaa tcttcatctg
    11221 atgaaggcta cttccctaaa attttctccc gtgtaaccaa agtggatgca ccggtgcagg
    11281 gaatgttgac cattgtgatt atccagagcg ggttggcgct gatgacgatt agcccgtcgc
    11341 taaacagtca gttcaacgtg ctggttaacc tggccgtggt gaccaatatc attccgtata
    11401 ttctgtcaat ggcggcatta gtcattattc agaaggtcgc taatgtgccc ccctcaaaag
    11461 cgaaagttgc aaactttgtt gcttttgttg gcgcgatgta tagcttttat gcgctgtact
    11521 catccgggga agaagccatg ctgtacggtt ccatcgtgac cttccttggt tggacactgt
    11581 atggtctggt atcaccacgc tttgaactga aaaataaaca cggttaaaaa agcaaagtaa
    11641 aaaagggcga tcttgcgacc gctctttttt tattaaatgt gtttacgcgt ttttcagaac
    11701 ttcgctaaca atctcaaccg cttctttctc aatctgcttg cgatgttctt caccgaggaa
    11761 gctttcgcag tagatcttat atgcgtcttc cgtgcctgac ggacgcgcgg cgaaccagcc
    11821 gttgtcagtc atcactttca gaccgccaat agaagcaccg ttgcccggag cagcagtcag
    11881 gcgcgcggtg atcgggtcac ctgccagggt gctggcgctc accatttccg gagacagctt
    11941 agacagcgcc gctttttgtg cggaagtcgc agctgcctgc aaacggttgt agctcggcgc
    12001 accaaagcgt tttgccagtt cgttgtagtg ttcctgcggg ttcttaccgg tgacagcggt
    12061 gatttccgcc gccagcagac acataatgat gccgtctttg tcggtggacc acggcgtgcc
    12121 gtcgaaacgc aggaaggaag cccctgcgct ctcttcgccg ccaaagccga agctgccgtc
    12181 gaacagacca tcaacaaacc atttgaaacc taccggtact tctaccagct tacggcccaa
    12241 gtcattgacc acacggtcga tcatcgcaga agaaaccagc gttttaccga cagcaacatc
    12301 tttgccccac tgcggacgat gctggaacag gtagttgatc gccaccgcca ggtagtggtt
    12361 cggattcatc aaacctgccg gagtgacgat accgtgacgg tcataatccg ggtcgttagc
    12421 aaacgccaga tcgaacttat cacgcagtgc cagcagaccc gccatcgcac actcggagga
    12481 gcagtccata cggatcgcac cgtctttatc gaggtgcata aagcggaagg tttgatcgac
    12541 ctgatcgtta acgatggtca ggttgaggtt gtaatactcg ccaatgcgct tccagtattc
    12601 gataccggaa ccgcccaacg gatcaacgcc cagcgtcagg cccgctttct gaatcgccgc
    12661 catatcaacg atatcggcca gcccttccac gaacggctgc accagatcct gctctttcac
    12721 atagccggat gccatcgctt cgtcgaggga gatacgcttc acgcctttca ggccatcggc
    12781 catcagtgcg ttggccttgt cttccaccac tttggtgacg ttagtatcag ccgggccacc
    12841 atttggcgga ttgtacttga taccaccatc ttccggcggg ttatgggacg gtgtaatcac
    12901 gataccgtct gccagcgggc cacctttttt attgtgaacc aggatggcat tggaaacggc
    12961 aggcgtcgga gtgaagccat tgttttcctg cacaatgaca tcaacgccgt tcgctgccag
    13021 cacttccagt acggaaatga acgcaggctc ggacagggcg tgagtatctt tacccacata
    13081 gcaagggcca gtgatgccgt ttttcgcacg ttcttcagca attgcctgag cgatcgccag
    13141 aatgtgcggc tcgttaaagc tgtggcgcgc tgcactgcca cggtgaccgg aagtaccgaa
    13201 tttcaccgcg tgctccgcat tccctgcttc tggtttcagt acataatatt gcgccgtcag
    13261 ttgggcgacg ttaatcaaat cactctgttg tgcaggttgg cctgcacgat tgtggattgc
    13321 cattgctttg tcctttgtct gcaacgtttt agatagttcc gcaaaccttc tcaatcaatt
    13381 ccgccgggaa ttgcatcgac tgcatgatgt gttcgatcat gctgcattta cggccggtgt
    13441 tggtgttggt gatcacccaa tacggcgtgc ctggcacatg tttcggcttg gtctgattac
    13501 catttttcag cagcgtttgt tcatctgccg caaagtacac gcgtgtacga ccgtgcaacg
    13561 attccgttgc ttcggcaaac gcctgggcgt caagagaata tagtgtagac aacagcagca
    13621 taaagcgatt gaccgctcgc ttttgctctg cgtattcgtc cgaaagcaga agttcacgca
    13681 ttgcgcgaac cttgtcttta atcgttttga ccggcttcgc ttcgacgata gcaggtgacg
    13741 caacgcgaac ctctttcgtc accggagcag caggctgtga tgcggcggaa aatttcaaca
    13801 tacgccgtaa aatgtcggat gcgctctcgc cgatatgctt agtgtggctg gcaatatagc
    13861 tgtagagttc atcatcaact tcaatcgttt tcatcttaat ccagtgcagt gtcttagctg
    13921 aatacaagtc gccaagagta tatgaataaa tcccggcagc ggatagcgtc aagcctggct
    13981 tgcagaaaaa ctcggaataa accttatttg ttgcagccct gtggcacaat ggtcaacata
    14041 ataccctaac ccgacaacgc gaaaaaaaga actttgccat gaaattaaat atccgcgcgc
    14101 aaactgcaca aaaccagcac aataattctc ccatcgttct tgtccatggt ctgtttggta
    14161 gcctcgacaa ccttggcgta ctggctcgcg atctggtaaa cgatcacaat atcatccagg
    14221 ttgatatgcg taaccacggt ctttcaccga gagagccggt aatgaattac ccggcgatgg
    14281 cgcaggatct tgttgatacc ctggatgcac tgcagatcga caaagcaaca tttatcggtc
    14341 actccatggg cggtaaagcg gtaatggcac ttactgcact ggcccccgat cgcatcgata
    14401 aactggtggc gatcgatatc gcaccggtcg actatcacgt acgccgtcat gatgagattt
    14461 tcgcagctat caacgcggtc agtgaatcgg acgcacaaac tcgccagcaa gcagcagcaa
    14521 taatgcgcca gcatcttaat gaagaagggg tgattcagtt tctgttgaaa tcttttgttg
    14581 acggggagtg gcgctttaac gtgccggtat tgtgggatca gtatccgcat attgtgggtt
    14641 gggagaaaat cccggcatgg gatcaccctg ccctgtttat ccctggcggc aattctccgt
    14701 atgttagcga gcagtaccgt gatgatttac tggcacaatt tccacaggca cgggcgcatg
    14761 tgattgcagg cgcgggtcac tgggtccatg ctgaaaaacc ggatgcggta ttacgcgcta
    14821 tccgtcgcta tctcaatgat taacgcaact gattaaaaac ccagcgtccg cgtcgctttc
    14881 cgcgcgtggg cgttggcgtg actcatcgcg ctgatgtatt atggcgcgct atccatccgg
    14941 gcagaggcct ggctgattgt ttcccccgaa gtcaccaaga tcatggccaa agaacaaacg
    15001 gaccgtacga cattagatct gttcgcgcac gagcgtcgac cgggacgacc gaaaactaat
    15061 ccgctttcgc gcgatgaaca gctgcgtatt aataaacgca accagctaaa acgcgacaaa
    15121 gtacgtggcc ttaagcgtgt cgaactgaag ctgaacgcgg aagctgtcga ggcgctgaac
    15181 gagctggcgg aatcgcgtaa tatgagccgt agcgaactga tcgaagagat gttaatgcag
    15241 caactggcgg cgctgcgtag tcagggcatc gtttaaattt ccactttcat gtagcacagt
    15301 gtgcagtcct gctcgtttgc tgattccgca agactgcctg ttctgctatg attgccttta
    15361 tccgtgggca attttccacc cccatttcaa taagtttcaa gaggttattt cactcatggc
    15421 tatcactggc atctttttcg gcagcgacac cggtaatacc gaaaatatcg caaaaatgat
    15481 tcaaaaacag cttggtaaag acgttgccga tgtccatgac attgcaaaaa gcagcaaaga
    15541 agatctggaa gcttacgaca ttctgctgct gggcatccca acctggtatt acggcgaagc
    15601 gcagtgtgac tgggatgact tcttcccgac tctcgaagag attgatttca atggtaaact
    15661 ggttgcgctg tttggttgtg gtgaccagga agattacgcc gaatatttct gcgacgcatt
    15721 gggcaccatc cgcgacatca ttgaaccgcg cggtgcaacc atcgttggtc actggccaac
    15781 tgcgggctat catttcgaag catcaaaagg tctggcagat gacgaccact ttgtcggtct
    15841 ggctatcgac gaagaccgtc agccggaact gaccgctgaa cgtgtagaaa aatgggttaa
    15901 acagatttct gaagagttgc atctcgacga aattcttaat gcctgatgtg atgcggcgta
    15961 gactcatgtc tacgccgtat taatagataa tgccaatcaa aataattgct acaaatttgt
    16021 aacttttgct gttgtaccta tacaatgtcc cagtgttcaa gtggccttgc cgttgtaaat
    16081 gtaagctgtg ccacgttttt attaacaata tttgccaggg acttgtggtt ttcatttagg
    16141 cgtggcaatt ctataatgat acgcattatc tcaagagcaa attctgtcac ttcttctaat
    16201 gaagtgaacc gcttagtaac aggacagatt ccgcatgact gataacaata ccgccctaaa
    16261 gaaagctggc ctgaaagtaa cgcttcctcg tttaaaaatc ctggaagttc ttcaggagcc
    16321 ggacaaccat cacgtcagtg cggaagattt atacaaacgt ctgatcgata tgggtgaaga
    16381 aattggtctg gctacggtat atcgcgtact gaaccagttt gacgacgctg gtatcgtcac
    16441 ccgccacaat tttgaaggcg gtaaatccgt atttgaactg acacagcaac atcaccacga
    16501 tcacctgatt tgcctcgact gcggcaaggt tatcgaattt agtgatgatt ccatcgaagc
    16561 gcgtcagcgt gaaattgccg caaaacatgg cattcgcctg actaaccaca gtctctatct
    16621 ttacggtcac tgtgccgaag gcgattgccg cgaagatgag cacgcgcacg aaggcaaata
    16681 agccagcctg aacgagaaaa gccaacctgc gggttggctt ttttatacaa gggaaataag
    16741 aaaagcgaga gttacagctc tcacttattt gttattactg cgtatttcct gccagacctt
    16801 atcacaatcg gctttcaccg cctgatcatt tccggtttgc gttgcacgca gacactgctg
    16861 ataatccagt acgcgtacac tttcctggtc ggcaaactgc tgatgttgtt tctctttctt
    16921 cagcacgttt aacacgctct ggcaggcttc aattttttct ggcgaacctt gcgcggtgtt
    16981 gatacaggcg ctgtatgcct ctttcagacg gctgtcttcc tgtggtgcgg ttgattgcgc
    17041 acaagctacc agccccgatg ccatcatggc gatgagaatc agttttttca ttgaatgcgg
    17101 caggcacaaa acctgccgcg tcgggcatca gaagatggtg aatggtgcga ttaccataaa
    17161 tttcacgtca cgctcatcct ggaagatgtt accgtaaccg ccgccccagc ttgggatgtc
    17221 ggagtggttg tcgtattcgg tgaagtgcag tttgaacatc gtgcctttgg cgcgaccgtc
    17281 ctgaatggtg tagaccgcat ccaggctgta ggcagactct tcaatagtcc ggtttttgtc
    17341 gtagtacgca tccggattgc tctgccaggt cgcaggttta gcatcccatg cgtaaacgta
    17401 ggaagcgccg atggcgaagc ctggaagatt ccagtttttc aggtcataca tcgcactgaa
    17461 gaagaccgct ttttcgccgt tagcgttgaa gtcggaacgg ttatcccacc agatatccag
    17521 gcggccgttg gaggaagcgt aggttggggt catacgttgc aggaagtatc cctgctgact
    17581 gtcagcctta atccaggtgc cttcgaggcg caaatccact acgtcagccg cccggtaacc
    17641 aaaggtcaac gcttgcagcc aggcggtgcc gtcataaaga tcgttgacgc tgcgatcgtc
    17701 aactttatcg cgggtaccat agaactggta gctggtggtt aacgggctac cggcgatatc
    17761 aaatttgtag ctggctttgg caaaatattg atcgatatac ccttccgcct gaccaaatgc
    17821 cgcttccagt acgaagttat ttttgaagtc gtatttcgca ccgatggagt gcagataatc
    17881 aactttggtg gttttatcgt tctgataaaa ctcatccatt tccagatgcc acggcgcttt
    17941 gtattcgttg gtccacatgt aggagaaact caaggcacca gcatcgccgt aatcaaaatt
    18001 cgccccggct tccgccccct gataagtacc cggcataaag ctccagtgcg gcgctaacag
    18061 cgtttgaccg gttggctgaa tgtaacctgc cctcgcccaa accggaccgt atttaaattt
    18121 ggccgcagct ttatacaggc ttataccgct tttgtcgccg gaccagtctt cgtcataggc
    18181 tttattactt tttgaaaacg cgatttcgtt cgggtgggag ctgtcgccgt tttccgccat
    18241 ttcaatcgcc gtaaacgcag caatatcaag gccgaacata tcagcagcat aaccggactg
    18301 aaaatcgagg ttggcattcc aggtggagtg agaaaggttg gttttgtatt tgtcgccgtc
    18361 ggtaacatct ttacggtcgc gttcacgctg ccagtaatag ataccgccgg ttaaggttga
    18421 atcgtcgatg aatccttcgg cgcgagcctc cggcattgcc ataaagcccg acattgctgt
    18481 aacaccggca atagccagcg ccagcgtact acgtttgcca ctaaacgtac gcatgggtta
    18541 atcctctttg acgtataaat tgctgcacca aaggtgaaca gcgaaaaaaa ataaaaatta
    18601 aaaaagtagc gttgagaaaa gtctcgctga ctacaggaac agactatttt tcgcgacgcg
    18661 aattatcaat ctctttctgt aaccagaatg taagaacatg agcaaccgca catattttga
    18721 tgatttttgt tacaaggctt aagtttgcgg ttgaaaggct tgcagaagaa taaaaacgtg
    18781 aaattccagc aagatgaaag cggttacatt ttatttaatt atgttttgtg attttaaatt
    18841 tattttatgg ataaattgcg aagcggaatt aattcgcgaa gaaaagcaga aaaaacgccg
    18901 cagagcgcgg cgtttgggat tgtttgctat ttcttgccgg atgcgg
//
LOCUS       DAJTSI010000077        18836 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000077 DAJTSI010000000
VERSION     DAJTSI010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18836
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.077"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            383..1093
                     /locus_tag="OO327_002393"
     CDS             383..1093
                     /locus_tag="OO327_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR6018908.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(1143..2117)
                     /gene="ghrB"
                     /locus_tag="OO327_002394"
     CDS             complement(1143..2117)
                     /gene="ghrB"
                     /locus_tag="OO327_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR6018909.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(2221..2871)
                     /locus_tag="OO327_002395"
     CDS             complement(2221..2871)
                     /locus_tag="OO327_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR6018910.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            3034..5232
                     /locus_tag="OO327_002396"
     CDS             3034..5232
                     /locus_tag="OO327_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR6018911.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALAGGYTGHLGDY
                     STGAAQAIMPYVVGGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGL
                     SYFSALRDSGKKLICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWH
                     DEAFLARCTTGYAVFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTML
                     MAGWGMQRQQFGEQKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGDPTRRSAVLSSM
                     QGSLPGGCDAVDKIPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQD
                     TNRLIRAWQKPELVVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMK
                     QVVPPRYEARNDFDVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVE
                     LPPFAEFWQANQLIEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYG
                     YPDCPGHPMWLEPDEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVT
                     IHPDDAQARGITEGDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGI
                     CKNGAVNVLTKDLPSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(5201..5641)
                     /locus_tag="OO327_002397"
     CDS             complement(5201..5641)
                     /locus_tag="OO327_002397"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6018912.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(5638..6201)
                     /gene="tag"
                     /locus_tag="OO327_002398"
     CDS             complement(5638..6201)
                     /gene="tag"
                     /locus_tag="OO327_002398"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR6018913.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            6359..7057
                     /locus_tag="OO327_002399"
     CDS             6359..7057
                     /locus_tag="OO327_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6018914.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            7286..8494
                     /locus_tag="OO327_002400"
     CDS             7286..8494
                     /locus_tag="OO327_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6018915.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            8818..10509
                     /gene="eptB"
                     /locus_tag="OO327_002401"
     CDS             8818..10509
                     /gene="eptB"
                     /locus_tag="OO327_002401"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR6018916.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            10601..10677
                     /locus_tag="OO327_002402"
     tRNA            10601..10677
                     /locus_tag="OO327_002402"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10635..10637,aa:Pro,seq:cgg)
     gene            11421..13028
                     /gene="dppA"
                     /locus_tag="OO327_002403"
     CDS             11421..13028
                     /gene="dppA"
                     /locus_tag="OO327_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCR6018917.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            13336..14355
                     /gene="dppB"
                     /locus_tag="OO327_002404"
     CDS             13336..14355
                     /gene="dppB"
                     /locus_tag="OO327_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR6018918.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            14365..15267
                     /gene="dppC"
                     /locus_tag="OO327_002405"
     CDS             14365..15267
                     /gene="dppC"
                     /locus_tag="OO327_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR6018919.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            15278..16261
                     /gene="dppD"
                     /locus_tag="OO327_002406"
     CDS             15278..16261
                     /gene="dppD"
                     /locus_tag="OO327_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR6018920.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            16258..17262
                     /gene="dppF"
                     /locus_tag="OO327_002407"
     CDS             16258..17262
                     /gene="dppF"
                     /locus_tag="OO327_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR6018921.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(17292..18563)
                     /locus_tag="OO327_002408"
     CDS             complement(17292..18563)
                     /locus_tag="OO327_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6018922.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         4472 a         4712 c         5014 g         4638 t
ORIGIN      
        1 taagaatagt cgggaaaagc agacacagcg gggtgaagtc agggcatgct ctggctatga
       61 ttcgattcag cctgtcattt agcgtgactt atagccacct aacgatctga taaatgccga
      121 tatatctctt atcactcctg ttttgtaaag atttcgcaaa tctaaccttc tgcgaaggcg
      181 taaatctctg gctacaaaaa aggcaacggc actatcattt tttaacaatg aaacaacaaa
      241 ttaaccttgt ttcattttct ccatgatttt accaggaaga ttacgtagaa agggtatcct
      301 gcaagcctgt cctggactat ccttgtcacg tcagacacgc gtgtgtcgtt gtgcgctttt
      361 tttgggtgaa aggagtaaga aaatggcgac aggaaagtcc tgctctcgct ggtttgcgcc
      421 tcttgcggcg ttattaatgg tagttagcct gagtgggtgt tttgataaag aaggcgatca
      481 gcgtaaagcg tttatcgact tcctgcaaaa tacagtgatg cgtagcggtg aacgtctacc
      541 aaccctgact gccgatcaga aaaaacagtt tggtcctttt gtctctgatt acgcgattct
      601 gtatggttat tctcagcagg tgaatcaggc gatggattcc ggtctgcgcc cggtcgtaga
      661 cagcgttaac gcaattcgcg taccgcagga ttacgttacg caaagcggtc cgctgcgtga
      721 aatgaatggt tcgctgggtg tgctggcgca acaactgcaa aatgcgaagt tgcaggccga
      781 tgctgcgcac tctgcgctaa aacagagcga tgacctgaaa ccggtatttg atcaggcatt
      841 caccaaagtg gtaacgacgc cagctgatgc attgcaaccg ttgattccgg cggcacaaac
      901 tttcacgcaa caactggtga tggttggaga ctacattgct cagcaaggta ctcaggtgag
      961 ctttgtcgca aatggcatcc agttcccgac ttcacagcag gcaagtgaat ataacaaact
     1021 gattgcgcca ttaccggcac aacatcaggc gtttaatcag gcctggacta cagcagttac
     1081 tgcaacccaa taaagagtaa aagcccgagc gattatcggg ctttacttca cgcagtcgca
     1141 gcttagtccg cgacgtgagg attcacacag ttcttctcaa cctttccttg taacgcatca
     1201 atcaaattat ccacggcaca ggcggccatg ccataacgcg tctcatgggt ggcagatcca
     1261 atatgcggta ctgcgacgac gttggccatt gagagcaacg gcgaatctac ggacagtggc
     1321 tcttgttcga agacatccag cccggcggcg tgaatttcgc ctttctgcaa tgctgcgatc
     1381 agtgcatttt cgtcaaccac cgggccacgt ccggcattaa tgaaaatggc ggaggatttc
     1441 attttggcga attgttctgc gccaaacaga tgatgcgtct catcagttaa cggcaggatc
     1501 aggcaaacga aatctgactc ttgtaacaga gtatccaaat cgcagtagcg ggcgttgaag
     1561 cgttcttctg cttctttatg gtggcggcgc gcgttataga ggatgggcat gttgaagcca
     1621 aagtgcacac gttgtgccag cgccatgccg atccgtccca tcccgacaat gcccagtgtt
     1681 ttatggtgaa cgtcagtgcc gtaccagtcc gggcctatgc tcgcggtcca ttcgcctgct
     1741 tttacccgtt ctgctacctc cacaacccga cgagcggtag acaacaccag cgccatcagc
     1801 gtatcggcga cggtttctgt taatacggtt ggcgtgtgca tcagcagaat ttttcgggcg
     1861 gtaagcgcat cgacatcaaa attgtcatag ccgacggaga tcgttgatgt ggcacgcagt
     1921 ttcggcattt tttccagcaa tgcggcatct acattctcgt ttgaacccag taaaccttca
     1981 gcttctgcaa aaattgctgc attttgaacg acggtttgtg ggctgaggtt tgccacctgg
     2041 tgaacggtga aatgcgcttg caggcgttgc agtaaatcat caggtaaggc tttgtagagg
     2101 ataacggacg gcttcatgct tctctccatt cactgataag gcgatcacct tagcctgaaa
     2161 tatgatgaag gaaaactgaa gaatgccagg tgatttctgc atcacctggc atgaaaggga
     2221 ttacagcggg cttaaggtaa tttctacacg gcggttttgc gccttacctt ctgcggtgct
     2281 gttgctggcg attgggttag ccgggccaag gccctgagta cggatgcggc tggcgtccac
     2341 gccctgggtg atcaacgcgc tggcaacgga atccgcacgc tgctgggaga gacgcatgtt
     2401 caggtcgtga ccccccgtgc tgtcggtata accaatcacg ttaaccgccg ttttcggata
     2461 ctctttcagc accattgcca cgccggtcag ggtgttagcg cctgccggtt tcaggggagc
     2521 gctgctgctg tcgaaggtca cattgttcgg catattgagg ataatgttat ccccgctgcg
     2581 ggttacgcta acaccagtgc cgcgcatttt gtcacgcagc ttcgcttcct gcacatccat
     2641 gtaataaccc acgccgccgc ccagagctgc gcctgctgct gcgccaatca gcgcgccttt
     2701 accgcgatct ttcttcgaag aagagagcgc accaataccc gcgcccacga gagagcccag
     2761 acctgcgccg atagcagatt tgcctgcttc gcgttcgccg gtgtaagggt tagttgtgca
     2821 gccagatacc gccagagcgc cactcactac ggcggcaagt aagataaaca cgtttcttca
     2881 ttgttaatcc ttaataacct ttttattctt tgtcacgggt tccgtggcgg gagattatgc
     2941 cgcgtgaaca tgaagattat tcctgggaat actcggaaat ttgtaagtaa tatttaactg
     3001 ctcaatacat ctaacctttc aggagtcttc ggtttggcca actcatcctc acgatattcc
     3061 gttctgactg ccgcccactg ggggcccatg ctggttgaaa ccgacggcga aaccgtgttt
     3121 agctcgcgtg gcgcgttagc cacaggaatg gaaaactcct tgcagagcgc ggttcgcgac
     3181 caggttcaca gcaatacgcg ggtacgattt ccaatggtgc gcaaaggctt tctcgcgtca
     3241 ccggaaaacc cgcaaggcat tcgtgggcag gatgaatttg ttcgcgtgag ttgggatgag
     3301 gcgctggcag gcggttatac cgggcatctg ggggattatt cgaccggtgc ggcacaggcg
     3361 atcatgccgt atgtcgtagg tggcagcgaa gtttatcaac agcaaaccag ttggccgctg
     3421 gtgctggaac atagcgatgt cgtggtgttg tggagtgcta acccactcaa tacgctgaaa
     3481 attgcgtgga atgcatccga tgagcagggg ctttcttact tttccgcact gcgtgacagt
     3541 gggaaaaagc tgatctgcat tgatccaatg cgatcggaaa ccgtcgattt ctttggcgat
     3601 aaaatggagt gggtggcacc gcacatgggc accgatgttg cgctgatgct ggggatcgcc
     3661 catacgctgg tggaaaatgg ttggcacgac gaagcgtttc tggcgcgttg caccacaggt
     3721 tatgccgtct tcgcctctta tttgctgggc gagagtgacg gaatagcgaa aaacgccgaa
     3781 tgggcggcag agatttgtgg tgttggcgca gcgaaaatcc gcgagctggc ggctattttc
     3841 caccaaaata ccaccatgct gatggcaggc tggggaatgc agcgccaaca gtttggtgag
     3901 caaaaacact ggatgatcgt cacgctggca gcaatgttgg ggcaaatcgg cacacccggc
     3961 ggcggttttg gtctttctta ccattttgcc aatggtggtg accccacgcg gcgttctgcg
     4021 gtgctctctt ccatgcaggg cagcttgccg ggtggctgcg atgcggtgga taaaatccct
     4081 gttgcccgca ttgttgaagc actggaaaac cctggtggcg catatcaaca caacggtatg
     4141 aaccgacatt tcccggatat tcgttttatc tggtgggcgg gcggtgccaa ctttactcat
     4201 catcaggata ccaatcgtct gatccgtgcc tggcaaaaac cggagctggt ggtgatctct
     4261 gaatgcttct ggacggcggc ggcgaaacac gcggatattg ttctgcctgc gactacctct
     4321 tttgagcgta atgatctcac catgaccggt gattacagta atcagcatct ggtgccgatg
     4381 aagcaagtgg tgccgccacg ctatgaagcg cgtaatgatt ttgatgtttt tgccgagttg
     4441 agtgaacgtt gggagaaggg cggctatgcg cggtttacgg aaggaaaaag tcagctgcaa
     4501 tggctggaaa cgttttataa cgttgcccgg cagcgcgggg caagccagca ggttgaattg
     4561 ccgccatttg ctgagttctg gcaagccaac cagttaattg agatgccgga aaacccggac
     4621 agcgagcggt ttattcgctt cgctgatttt tgccgcgatc cgctggcgca tccgttaaaa
     4681 accgccagcg gcaagattga aatcttctca cagcgtattg ccgattacgg ttacccggat
     4741 tgccctgggc atccaatgtg gctggagccg gacgaatggc agggcaatgc cgaaccagaa
     4801 cagttgcagg tactttctgc ccatccggcg caccgcctgc acagccagct gaattacagt
     4861 tctctgcgcg aattgtacgc ggtagcaaac cgtgagcctg tcaccattca tcctgacgat
     4921 gctcaggcgc gcggcataac cgaaggggat atggtgcggg tgtggaactc ccgtgggcag
     4981 atccttgccg gagcggtcat tagcgaggga attaaacctg gcgtgatttg cattcacgaa
     5041 ggggcatggc cggatctgga tttaaccgct gacggtattt gtaaaaacgg cgcagtgaac
     5101 gtgctgacca aagatctccc cagctcgcgg ctggggaatg gctgtgcggg taatacgacg
     5161 ctggcatggc tggaaaaata caacggtccg gaactgacac ttacagcgtt tgaaccaccg
     5221 gccagctcat aatccaggtg ggtagttggg tttcatcctg ccatgcgcaa tcgacaatgt
     5281 gaaaaccctg cgcacggtaa aaatctatcg ccggttgatt tttttgataa acctccagca
     5341 tcagatgggg atagcgctgc tgcacatact gcatcagcgc cttaccaata ccgcgtctga
     5401 cggccttcgg tgcgacaaac attgctgcca gaaatcggcc ttccataatg ctgacaaaac
     5461 cgagaagctt accgtcctct tcccagaccc agttttgcgc gttggcaaga taggcatccc
     5521 gcaccagcgg aatgcagtca cgccagtaat tcgcttttat aaagggatgc ccccaggttg
     5581 tactttccag ccacagttcg aggatcgcgg ggagttctga acgttgcgct tcccgaatca
     5641 tggtttattt cccagatagc aacagcagcc aaccacatga tcattcacca gcccacatgc
     5701 ctgcataaag gagtaacaga ttgtggtgcc gacaaactta aaaccacgtt ttttcagtgc
     5761 cttagatagg gcgtcggagg cggacgtaga tgtgggaatt tcgctcaacg ttgtggcttg
     5821 tgtcacctgt ggctgatgat ttacaaacga ccagacaaag tcgggaaacg gttcgccgtt
     5881 ctgttccatt tgcaggtacg cccgcgcatt accaataatt gcctgaattt tccctcgatg
     5941 gcggataatc ccggcgtcct gtaccagtct ttcgacatcc tcttcctgca ttgctgcgac
     6001 cttcaccgga tcgaactgat gaaagtaggc gcgatagttt tcgcgttttt tgaggacggt
     6061 gatccacgat aacccagcct gctgcccttc aaagcagatc atttcgaaca gttttttact
     6121 gtcagtttca ggcacgcccc actcattatc atggtaggca atataaagcg ggccctgact
     6181 cacccagccg caacgttcca tactttccct cgctatgtac ccgatcgtaa aaatttcagc
     6241 cgcttttctt gacgcatcgg tgaaaaagtc aatatttaat acagggctac tatgcccgat
     6301 atccttcata caatgacaat aatattgccg aattcggctc ttccctggag tcgtgttaat
     6361 gatcataaaa aaaagcggtg gtcgctggca gctaagcctg ctggcgagcg tggtaatcag
     6421 tgcctttttt ctcaacacag cttacgcctg gcaacaagaa tatatcgttg atacgcaacc
     6481 cggacattcc acagagcgtt acacctggga tagtgatcat caacctgatt acaacgatat
     6541 tttgtcgcaa cgtattcaaa gtagccaaag ggcgctggga ctggaagtca atctggcgga
     6601 agaaactcct gtggatgtga ccagcagtat gagtatgggc tggaattttc ctttatatga
     6661 acaggttaca accggcccgg tcgcggcatt acattacgat ggcacaacca cctcgatgta
     6721 taacgagttt ggcgacagta ctaccacgct gaccgatccg ttatggcatg ccagcgtgag
     6781 tagcttaggc tggcgtgttg actcccggct tggcgatctc cgaccctggg cgcaaatcag
     6841 ctataaccag caatttggcg agaatatctg gaaggcgcaa tcaggcctga gccggatgac
     6901 ggcgacaaac cagaacggca actggctgga tgtcaccgta ggcgctgata tgttgctcaa
     6961 tcaaaatatt gccgcctatg ctgcgctaac tcaggcagaa aataccacta ataatagcga
     7021 ctatctgtat acgatggggg ttagcgccag attttaacgt aacagtcaca attgaaacca
     7081 ttgaataaca acagttgtgg cgatagtggg tgctaactta ccaaataata aatttggtga
     7141 ataattgtcg cgtcattcat tcctgaacta aggcatttca ttccgttctg atggcatttc
     7201 atgccgtttt tccccaagca taaaatgcac ttcgttatgg ttgtcggcag agatttttcc
     7261 tttttattac tgcaggaata ctgccatgac accttcaaat tatcagcgta cccgctggct
     7321 gacactcatc ggtactatca ttacccagtt tgcgctgggg tcggtttata cccggagcct
     7381 gtttaatggc gcgctttctg ccaagctgga tgcgccggta agccaggtcg ctttctcttt
     7441 cggcttgtta agtctggggc tggcaatttc gtcttctgtt gcgggcaaat tgcaggaacg
     7501 ttttggcgtt aaacgcgtca ccgtggcttc cggcattttg ctgggattag gcttcttcct
     7561 gaccgcgcat tccaacaacc tgatgatgct gtggttaagc gccggtgtgc tggtcggtct
     7621 ggcggatggc gcgggttacc tgctgacgct ctctaactgc gtgaagtggt tcccggagcg
     7681 taaaggtctg atctccgcgt tcgctatcgg ttcttatggt ctgggtagcc tgggtttcaa
     7741 atttatcgac acgcagctgc tcgaaacggt gggtctggaa aaaacctttg tgatttgggg
     7801 agcgattgcg ctggtgatga ttgttttcgg cgcaacgtta atgaaagacg caccaaaaca
     7861 ggaagtgaaa accagcaatg gtgtggtgga gaaagattac acgctggcag agtcgatgcg
     7921 taaaccgcag tactggatgt tagcggtaat gttcctgact gcctgcatga gcggcctgta
     7981 cgtgattggg gtagcgaaag atatcgccca aagtctggca caccttgatg tggtttccgc
     8041 agccaatgca gtaacggtta tttccatcgc taacctttca ggtcgtctgg tgctgggcat
     8101 tctgtctgac aaaattgccc gtatccgtgt tatcaccatt ggtcaggtga tttcgctggt
     8161 gggtatggcg gccctgctgt ttgcaccatt aaatgcagtg acgttctttg cagcgattgc
     8221 ctgcgtggca tttaactttg gcggcactat taccgtcttt ccatcactgg tcagtgagtt
     8281 cttcggcctc aataacctgg cgaaaaacta cggtgtgatt tacctcggtt tcggtatcgg
     8341 tagcattttt ggttcgatta tcgcctcact gtttggcggt ttctatgtga ccttctacgt
     8401 gattttcgcc ctgctgattc tgtcattggc gctttctacg acgattcgtc agccagagca
     8461 gaaaatgttg cgtgaggcgc atggctccct ttaatgctca gccgcgagct actgggcttc
     8521 agcttccgtg tgcatcaaac gcatcaacga aatgtataaa caatgactcg taaaatttgg
     8581 gccagttaac tggccctttt tactgctttt tgtaacgcta aagtctcttt tcaaacttgc
     8641 atttttgtaa atttgtgctt catgcacact ctttccccac actttttcgc tttgctgtgg
     8701 tctacttatt cgcgcgtgta gattttactt atctgactac ctccgcactt ttcccctgcc
     8761 gggcctgaaa agccgctaag cagggtgtta tcacctgttt gtccagggtt tgtttgcatg
     8821 agatacatca aatcgattac acagcagaag ctgagctttt tgcttgcaat ctatattggc
     8881 ctttttatga atggcgcggt tttttatcgc cgcttcggca gctatgcgca cgattttacc
     8941 gtctggaaag gcgtttctgc tgttgttgaa ctggccgcca ccgtactggt gaccttcttt
     9001 ttactacgtc ttctatcgct gtttggccgc cgcagctggc gtattctggc gtcgctggtg
     9061 gtgctctttt ccgcaggtgc cagctattac atgaccttcc ttaatgtggt cattggttat
     9121 ggcatcatcg cttccgtcat gaccaccgat atcgacctgt caaaagaagt tgttggtctg
     9181 aactttattc tctggttaat cgccgttagt gcattgcctc ttatccttat ctggaataac
     9241 cgctgtcgct acaccttgct ccgacaactg cgaaccccag ggcagcgtat tcgcagcctg
     9301 gtggtcgtcg tactggcggg tattatggtt tgggcaccga ttcgtttgct ggatatccag
     9361 cagaagaaag tggagagggc gaccggcgtt gatttgccga gttatggcgg tgtcgtagcg
     9421 aactcttatc tgccatcaaa ctggctttct gcgttggggc tgtatgcctg ggcgcgggtc
     9481 gatgaatctt ccgataataa ttcattgctt aatccggcga agaagtttac ataccaggct
     9541 ccacaaaacg ttgatgatac ttatgtcgtg tttatcatcg gtgaaaccac gcgttgggac
     9601 catatgggta ttttcggcta tgagcgtaat accacgccga aactggccca ggagaaaaat
     9661 ctggcggcgt tccgtggtta ctcctgtgat accgcaacca aactctcact gcgttgcatg
     9721 tttgtacgtc aggggggcgc ggaagataat ccgcagcgca cattaaaaga acagaacatt
     9781 ttcgcggttc tgaagcagtt aggattcagt tctgacctct acgctatgca gagcgaaatg
     9841 tggttctaca gcaacacgat ggcagacaac attgcttatc gtgagcagat tggtgcggag
     9901 ccacgtaatc gtggcaagcc ggtagatgat atgttgctgg tagacgaaat gcagcaatcg
     9961 ctagggcgca acccggatgg taagcatctg atcattctgc ataccaaagg ttcgcatttt
    10021 aactacaccc agcgttatcc gcgtagcttc gcgcagtgga agccggaatg tattggtgtt
    10081 gatagcggct gtaccaaagc gcagatgatc aactcctatg acaactcggt gacctatgtg
    10141 gatcacttta tctccagcgt gattgatcag gttcgcgata agaaagcgat tgtgttctac
    10201 gcagctgacc acggtgagtc aattaatgaa cgcgagcacc tgcacggcac gccgcgtgaa
    10261 ctggcaccgc cggagcagtt ccgcgtaccg atgatggtct ggatgtcaga taaatatctg
    10321 gaaaatccgg ccaatgcgca ggcgtttgcg cagctgaaaa aagaagccga catgaaagtg
    10381 ccacgccgtc acgtagagct gtacgacacc atcatgggtt gtcttggcta tacttcaccg
    10441 gatggtggaa ttaacgaaaa caacaactgg tgtcacatcc cgcagacaaa agtggcagcg
    10501 gctaactaag cggcctgctg actttctcgc cgatcaaaag gcattttgct attaagggat
    10561 tgacgagggc gtatctgcgc agtaagatgc gccccgcatt cggtgattgg cgcagcctgg
    10621 tagcgcactt cgttcgggac gaaggggtcg gaggttcgaa tcctctatca ccgaccaaat
    10681 tcgaaaagcc tgctcaacga gcaggttttt ttgcatttgt agctcatgag gatgagaacc
    10741 tccgggggca gggaggctcg actcgacgta ggcctgataa gacgcgccag cgtcgcatca
    10801 ggcaacggca ccgcacttat tgtcggatgc ggcgcgaccg ccttattcga ccaacacacc
    10861 acacattttc ctgccaactt tgcgccccat atctcactat ccattaacgg atttgtgaca
    10921 gaaaccattg tcttttttta tatttgctta aatctttttt gcgttactta tggctggcgc
    10981 tggcattttc cgctgtgcgt tttagcgttc atggtattaa tcgctcagat atccaccttt
    11041 tcctcagatt tttttcatta aatgcataaa acttaatcac caaatgttgc taaatattaa
    11101 gctgattgtt ctggttatgt tggagtagca caaatttctc tgctgcaaat agctgtttga
    11161 agttttttta atctttgttt gtggattctc accgttggtg taaatcccgg ttggctgtat
    11221 tgacgttttc acattctgtt gacagattgt aggtcacgag gggcatttta tggaggatcc
    11281 gcactgttac actgatgtta attagtacgg catccccacc tcataacgtt gacccgaccg
    11341 ggcaaaaaac aaaaaaggtc aggcagcgac aacccactgc aaagggttaa aacaacaaac
    11401 atcacaattg gagcagaata atgcgtattt ccttgaaaaa gtcagggatg ctgaagcttg
    11461 gtctcagcct ggtggctatg accgtcgcag caagtgttca ggctaaaact ctggtttatt
    11521 gctcagaagg atctccggaa gggtttaacc cgcagctgtt tacctccggc accacctatg
    11581 acgcctcttc cgtcccgctt tataaccgcc tggttgaatt taaaatcggc accaccgaag
    11641 tgatcccggg cctcgctgaa aagtgggaag tcagcgaaga cggtaaaacc tataccttcc
    11701 atctgcgtaa aggtgtgaag tggcacgaca ataaagaatt caaaccgacg cgcgaaatga
    11761 acgccgatga cgtggtgttc tcgttcgatc gtcagaaaaa cgcgcaaaac ccgtaccata
    11821 aagtttctgg cggcagctac gaatacttcg aaggtatggg cttgccggag ctgatcagcg
    11881 aagtgaaaaa ggtggacgat aacaccgttc agtttgtgct gactcgaccg gaagcgccgt
    11941 tcctcgctga cctggcaatg gacttcgcct ctattctgtc aaaagaatat gctgacgcga
    12001 tgatgaaagc cggtacaccg gaaaaactgg acctcaaccc aatcggaacc ggtccgttcc
    12061 agttacagca gtaccaaaaa gattcccgta tccgctacaa agcgtttgat ggctactggg
    12121 gcaccaaacc gcagatcgat acgctggtct tctctattac ccctgatgct tccgtgcgtt
    12181 acgcgaaatt gcagaagaat gaatgccagg tgatgccgta cccgaacccg gcagacatcg
    12241 ctcgcatgaa gcaggataaa tccatcaacc tgatggaaat gccggggctg aacgtcggct
    12301 acctctcgta taacgtgcag aaaaaaccgc tggatgacgt gaaagttcgc caagcactaa
    12361 cctacgcggt gaacaaagac gcgatcatca aagcggttta tcagggcgcg ggtgtatcag
    12421 cgaaaaacct gatcccgcca accatgtggg gctataacga cgacgttcag gattacacct
    12481 acgatcctga aaaagcgaaa gccttgctga aagaagcggg tctggaaaaa ggtttctcca
    12541 tcgacctgtg ggcgatgccg gtacaacgtc cgtataaccc gaacgctcgc cgcatggcgg
    12601 agatgattca ggcagactgg gcgaaagtgg gcgtgcaggc caaaatcgtc acctacgaat
    12661 ggggtgagta cctcaagcgt gcgaaagatg gcgaacatca gacggtaatg atgggctgga
    12721 ctggcgataa cggggacccg gataacttct tcgccaccct gttcagctgc gccgcctctg
    12781 aacaaggttc taactactca aaatggtgct acaaaccgtt tgaagatctg attcaaccgg
    12841 cgcgtgctac cgacgaccat aacaaacgcg ttgaactgta caagcaagcg caggtggtga
    12901 tgcacgatca ggctccggca ctgatcatcg ctcactccac cgtgtttgaa ccggtacgca
    12961 aagaagtcaa aggctatgtg gttgatccat taggcaaaca tcacttcgaa aacgtctcta
    13021 tcgaataatt aaaagccata caagactgat ggcaaaggca aaactgcctg atgtgctccg
    13081 cttatcaggc ctacgaaaat tctgcaatgt attgaatttg cacgattttg taggccggat
    13141 aaggcgttaa cgccgcatcc ggcataaaca aagcgcactt tgtcaacaat ctgtataccc
    13201 ggtggcgctg tgcctctgcg tgcagcgcca tccggcagca atacttcatt ctctgtccga
    13261 caagggcggg aattgatttg tgagcaatac agacacgcag ttccaggctg cgagtcacta
    13321 tagagaatcc gggttatgtt gcagtttatt ctccgacgtt tgggactcgt catccccacg
    13381 tttatcggta ttacccttct cacatttgcc tttgtccaca tgatcccggg cgatccggtg
    13441 atgatcatgg caggcgaacg tgggatctcc cctgagcgtc acgtgcagct gctggctgaa
    13501 ctcggcttag ataaaccgat gtggcagcag tatctccatt acatttgggg cgtaatgcac
    13561 ggcgatctag gcatttcaat gaaaagccga attccagtat gggaagagtt cgtgccgcgc
    13621 tttcaggcca cgctggaact tggcgtctgc gcgatgattt ttgctacggc agtcggtatt
    13681 ccggttggtg tgctggctgc ggttaaacgc ggttccattt tcgatcacac tgcagttggc
    13741 ctggcgctga ccggctactc gatgccgatc ttctggtggg gcatgatgct gatcatgctg
    13801 gtttcggtgc actggaacct gacgcccgtc tccggtcgcg tgagcgatat ggtgttcctc
    13861 gatgactcca atccgttaac cggttttatg ctaatcgaca ccgccatctg gggtgaagac
    13921 ggcaacttta tcgatgccgt cgcccatatg atcttgcccg ccattgtgct gggtactatt
    13981 ccgctagcgg tcattgtgcg tatgacacgc tcctcgatgc tggaagtgct gggcgaggat
    14041 tacatccgca ccgcgcgcgc caaagggcta acccgcatgc gggtgattat cgtccatgcg
    14101 ctgcgtaacg cgatgctgcc ggtagtgacc gttatcggcc tccaggtggg aacattgctg
    14161 gcgggggcga ttctgaccga aaccatcttc tcgtggcccg gtctggggcg ctggttgatt
    14221 gacgcactgc aacgccgcga ctatccggta gtgcagggcg gcgtattgct ggtggcgacg
    14281 atgattatcc tcgtcaactt gctggtcgat ctgctgtacg gcgtggtgaa cccgcgtatt
    14341 cgtcataaga agtaagggga catcatgtca caggttactg aaaataaagt gattagcgca
    14401 ccggtgccga tgaccccgtt acaggagttc tggcactatt ttaaacgcaa caaaggcgcg
    14461 gtcgtcgggc tggtttacgt cgccatcgtg ctgttcatcg cgatctttgc caactggatt
    14521 gcgccttata acccggcgga tcagttccgc gatgcgctgc tcgccccgcc agcctggcag
    14581 gaaggcggca gtatggcaca cttgctgggc accgatgacg tcggtcgtga tgtgctgtcg
    14641 cggctgatgt acggcgcgcg tttatcgctg ctggttggct gtctggtggt ggtgttgtcg
    14701 ctgattatgg gcgttattct cggcctgatc gccggttact tcggcggcct ggttgataac
    14761 attatcatgc gcgtggtcga tatcatgctg gcgctgccaa gtctgctgtt ggcactggtg
    14821 ctggtggcga ttttcggccc gtcgattggt aacgccgcgc tggcgctgac cttcgttgcc
    14881 ttgccgcact atgtgcgctt aacccgcgcc gccgtgctgg tggaggtgaa tcgcgattac
    14941 gtcaccgcct ctcgcgtggc aggtgccggg gcgatgcgcc agatgtttat taacatcttc
    15001 ccgaactgcc ttgcgccgct gattgttcag gcgtcgctcg gtttctctaa cgccattctc
    15061 gatatggctg ctcttggttt cctcggcatg ggggcacagc cgccaacgcc tgagtggggc
    15121 accatgctct ccgacgtgtt gcagttcgcg caaagcgcct ggtgggtcgt gaccttccct
    15181 ggtctggcga tcctgttgac ggtgctggca tttaacctga tgggtgacgg tctgcgtgac
    15241 gcgctcgatc ccaaactaaa gcagtaagag gttcgagatg gcgttattaa atgtagataa
    15301 attatcggtg catttcggcg acgaaagcgc accgttccgc gccgtagacc gcatcagcta
    15361 cagcgtaaaa cagggcgaag tggtcgggat tgtgggtgag tccggctccg gtaagtcggt
    15421 cagttcactg gcgattatgg ggctgattga ttatccgggc cgcgtaatgg cagaaaaact
    15481 ggagtttaac ggccaggatt tgcagcgtat ctcagaaaaa gagcgccgca acctggtggg
    15541 tgccgaagtg gcgatgatct tccaggaccc gatgaccagc cttaacccgt gctacaccgt
    15601 gggtttccag attatggaag cgattaaagt gcatcagggc ggcaacaaaa gtacccgccg
    15661 tcagcgggcg attgacctgc tgaatcaggt cggtattccc gatccggctt cgcgtctgga
    15721 tgtttacccg catcagcttt ccggcggcat gagccagcgc gtgatgatcg ccatggcgat
    15781 tgcctgtcgg ccaaaactgc tgattgccga tgaaccgacc accgcgctgg acgtgaccat
    15841 tcaggcgcaa atcatcgaac tactgctgga gctacagcag aaagagaaca tggcgctggt
    15901 gttaattacc catgacctgg cgctggtggc ggaagctgcg cataaaatca ttgtgatgta
    15961 tgcaggtcag gtagtagaaa cgggcgatgc acacgccatc ttccatgcgc cgcgtcaccc
    16021 gtatactcag gcattgctgc gtgcgctgcc agaatttgct caggacaaag aacgtctggc
    16081 gtcgttgcca ggtgttgttc ccggcaagta tgaccgcccg aacggctgcc tgcttaaccc
    16141 gcgctgcccc tatgccactg acagatgtcg cgctgaagaa ccggcgctga atatgctcgc
    16201 tgatgggcgt cagtccaaat gccattaccc acttgatgat gccgggaggc ccacactatg
    16261 agtacgcaag aggccacctc gcaacaaccg ctgttgcagg ctatcgacct gaaaaaacat
    16321 tatccggtga agaaaggcat gttcgtgccg gaacgtctgg tgaaggcgct ggacggcgtt
    16381 tcgtttaacc ttgaacgtgg caaaacgctg gcagtagtgg gcgaatctgg ctgcggtaaa
    16441 tcgaccctcg gtcggttgct gacgatgatc gaaacgccca ccggcggcga gctgtattat
    16501 caggggcagg atctgctcaa gcacgatccg caggcgcaga agctgcgtcg gcagaaaatc
    16561 cagatcgtct tccagaatcc ctacggttcg ctgaatccgc gtaagaaagt tgggcaaatt
    16621 ctcgaagagc cgctgcttat taacaccagc ttaagcaaag aacagcgtcg ggaaaaagcc
    16681 ctgtcgatga tggcaaaagt cggcctgaaa accgagcact atgaccgcta tccgcacatg
    16741 ttttccggcg gacaacgtca gcgtatcgct attgcccgtg gtctgatgct cgacccggat
    16801 gtggtgattg ccgatgagcc ggtttctgcg ctggacgtct cagtgcgtgc gcaggtgctg
    16861 aatctgatga tggatttgca gcaggagttg gggctttctt atgtctttat ctcccacgac
    16921 ctgtcggttg tggagcacat tgctgatgaa gtgatggtga tgtacctagg ccgctgcgtg
    16981 gagaaaggaa cgaaggacca aatcttcaat aacccgcgcc atccgtacac ccaggcgctg
    17041 ctctccgcga cgccacgcct gaacccggac gatcgtcgtg aacgcatcaa actcaccggt
    17101 gaactgccaa gcccgctgaa tcctccgccg ggttgcgcct tcaacgcccg ctgtcgtcgg
    17161 cgcttcggcc cctgcaccca gttgcagccg cagctaaaag actacggagg tcaactggta
    17221 gcttgttttg ctgttgatca ggatgaaaat ccgcagcgtt aactcacaaa accggggcca
    17281 tgcgctccgg tttactcaat cagcttcaac agtggcgaca agcatagcaa aatgccgtaa
    17341 agcagaatca gccaggcttt aaatccgcga agtttttcca gttgtgcgac tttatagatc
    17401 aggaaaaacg gaatgaggca cgacacaatg ccatataacg ggcttcccaa ctgaaagaac
    17461 accagcactg atacacgaaa cgaaacccaa atcgtcaacg taatgacgat aaaagcgcag
    17521 atcgccagag tcagcatgcg tgagttaatt ttcttggtat caataattcg gcttaacaga
    17581 ttgagaataa tgcctttaat agcctcgtgg aaaccgagat aaatgccaaa gaatgcggtc
    17641 agtacggcaa agatattcag caccgtagag gtgatatgaa tgatatgtcc agggatcacc
    17701 tgcgcggcca gtgccagtgc cgagatattt tgttcaaagg cagaaacggc ttcttcgtgg
    17761 ctaattgaga aggtaaacga aaaggcaaaa aacaggatca ccgcgatgag cgtgatataa
    17821 ctaattcggt gggtacgcag cgcgagccgg gttgccagta ctttatctgc ttcccgttta
    17881 cgataggcaa tattcattgg gttaagtacc tgaataaata ccgcagaaaa gaagcaaaat
    17941 ggaatggtaa gcaggacatc gcggaaaaag acggaggctt gcgggaaggc ggttatattg
    18001 gcgaaattcc agtgcgggat catcgcaaaa ccgaacacga caataatccc tactttgacc
    18061 accaccattg gcccagaaat cttaaacagt aatcgttcac caccagacgc aatcgctatc
    18121 agtacggcga aaatagcgac tttataaagt agagattgtg aaagatcggc atcggttaaa
    18181 ccgaaggttt tcaggtacga ggcgctgtcg aaaaccacgg agagagagta gataaaaatc
    18241 ccgtggataa tcatcaaaaa gtagataacc ccgaggaaaa ttccccagtt cttccccagg
    18301 taatgactga taatatcggt gtagtcatta caggaatcgc tttcagaaag ggtttttaaa
    18361 taaatgtcct gcactaccca ggtgacagga taagcaatga tcgctgcggt aataaatacc
    18421 caaattccct tcaagccaat ttgtactggc atcagcacgg ttccggcacc aatcgccatg
    18481 cctatgcata ataaaaccca gccgaagtcg tagcgtgtaa acggcaattt ctgattgtgt
    18541 ttcgatagtg tgttgtgctg cataaagtcc tgtccagaaa gaagtcggcc tcgcaaaaat
    18601 gaataaaccc agtttctctt gcgcaacagg tggagaagtc gaatacgcag tatgtaaaaa
    18661 atgaggcaac agattcactg gggaggtcgc gaggaggcgc aattctacaa aattgcgcat
    18721 ttcactacca gtctggagtc agcccggata tgaactgcaa ctattgcaaa taaagaaatt
    18781 aagctggaag gcatcgcgca atgaggaaga ttgccgggtg ctgacagtaa aaagca
//
LOCUS       DAJTSI010000078        18803 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000078 DAJTSI010000000
VERSION     DAJTSI010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18803
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.078"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(139..3654)
                     /locus_tag="OO327_002409"
     CDS             complement(139..3654)
                     /locus_tag="OO327_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6018923.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLLVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQDISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4107..5369)
                     /locus_tag="OO327_002410"
     CDS             complement(4107..5369)
                     /locus_tag="OO327_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6018924.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
     gene            complement(5567..5642)
                     /locus_tag="OO327_002411"
     tRNA            complement(5567..5642)
                     /locus_tag="OO327_002411"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5607..5609),aa:Phe,seq:gaa)
     gene            complement(5748..6455)
                     /locus_tag="OO327_002412"
     CDS             complement(5748..6455)
                     /locus_tag="OO327_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR6018925.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            6853..8988
                     /gene="speC"
                     /locus_tag="OO327_002413"
     CDS             6853..8988
                     /gene="speC"
                     /locus_tag="OO327_002413"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR6018926.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFISMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="OO327_002414"
     CDS             complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="OO327_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR6018927.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="OO327_002415"
     CDS             complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="OO327_002415"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR6018928.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(11639..11914)
                     /locus_tag="OO327_002416"
     CDS             complement(11639..11914)
                     /locus_tag="OO327_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR6018929.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="OO327_002417"
     CDS             complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="OO327_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR6018930.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            13155..13874
                     /gene="trmB"
                     /locus_tag="OO327_002418"
     CDS             13155..13874
                     /gene="trmB"
                     /locus_tag="OO327_002418"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR6018931.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            13874..14200
                     /locus_tag="OO327_002419"
     CDS             13874..14200
                     /locus_tag="OO327_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR6018932.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            14384..15103
                     /locus_tag="OO327_002420"
     CDS             14384..15103
                     /locus_tag="OO327_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR6018933.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            15279..16325
                     /gene="ansB"
                     /locus_tag="OO327_002421"
     CDS             15279..16325
                     /gene="ansB"
                     /locus_tag="OO327_002421"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR6018934.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            16442..17404
                     /locus_tag="OO327_002422"
     CDS             16442..17404
                     /locus_tag="OO327_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR6018935.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKQDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(17505..>18803)
                     /locus_tag="OO327_002423"
     CDS             complement(17505..>18803)
                     /locus_tag="OO327_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR6018936.1"
                     /translation="DSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAA
                     ITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNV
                     LANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVL
                     IVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAF
                     LDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILES
                     VVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIF
                     MDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGV
                     KIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         4900 a         4584 c         4643 g         4676 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagctgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg atatcttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca tcaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcatcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaagagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aagcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcgcct gcggaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc tcaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt gggcgtcttt ccgtttaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcattttcat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aatcaccgta tggacagcta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcgatat caaatgaact
     3781 ggttgtacaa ggttcagaac acaagctcat taaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa taactacctg tgttggacgg ggataccaga
     3901 gcagccgaaa acaccggggg ttattacctg ctctcatgga aaccatatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacgt gacgaaggga ctttaatgga agccggagta
     4021 acttgtcaga aacatcaatc ttcgtcatca tttacaaaga aaaataatta gttaaagcag
     4081 tccgggctga ttgcccggac tgcttactat tttaatggat tgttaatctt tgcatattca
     4141 aacgggctga tacccttctc ccggttcgca tctagaaaat cggcccacca ttgcaacatc
     4201 agtcgccgtt cttccagatg ttctgcttta tggatatacg cagcacgtac accattacgc
     4261 tcctgatggc tcatctgacg ttccacagca tcgcgggacc acaaacctga ttcaatcaat
     4321 gcactgcacg ccatcgcccg gaatccatgg ccacagactt cctgggttgt gtcatacccc
     4381 atcaccctca gggctttatt tacagtattt tcactgattg gtttacgcgg gtcatgtgca
     4441 cccgtaaaga tcagaccatt ttcaccagcc catgtctgaa gttctgccag tatggctaca
     4501 acctgtttgc tgagtggcac aagatgtggt gttcgcatct tggatccccg gtgtgagaat
     4561 tttacgcctg gcagaggttc gcgttcggct ggaatagtcc acatggaatt gtcaatatca
     4621 atttccgacc agcgggcata tctgagttca ctggaacgaa taaaaaccag taaattcagc
     4681 ataactgcca gtcgggtgac aggctggcct ttataaccgt caatacgcga cagaagttca
     4741 ggcaggcgtg aaagctccag agcagggcga tgctggcgtt ttaccgtggt caatgcacca
     4801 gccatatcca gagccgggtt atatctgatg atgccagact gtacggcgta acgcattaca
     4861 gcagatattc tttgctgcag acgactggcg atttcataaa tttgttttgc ttctgcagca
     4921 cgaacaggga taagcaggtc cggggtattg agtgttgtga tatcccggtt gccgataaag
     4981 gggaaaacgt gtgtctcgag gcttttgagt actttatcgc tgtgtgattg tgcccattgt
     5041 ttattactgg catgccagcg tcgtgcaata tgttcaaaag agagctgtaa atcagggaca
     5101 acttttttaa ctcttttctg ttcgcaaggg tcaatcccct ctgaaatcag ttttcgggcc
     5161 tcatcccgtt ttgtacgagc ttcggagaga gaaacttctg gatacacacc aagtgccaga
     5221 gtcttctcct tacccagaat acgataacgg agacgccagt aacgggaacc attaggatga
     5281 accagcagga acatgccggc cccgtcagtg agtttataag ccttgtcagt gggctttgca
     5341 gcccggattt ttgcgtcagt cagtgccata gttcagcctt ctgaatgcgt taaagagggt
     5401 acaaattctg tcgaactggg gtatacccgc aattataccc acacaatggg gttgatgtgg
     5461 ggggaatctg gttgagttcg gtagaattga tttggaggaa gaacacttaa atcatggtgt
     5521 tctgaaacga aaaacggacc tccgtggagg tccgttgata tgaatttggt gcccggactc
     5581 ggaatcgaac caaggacacg gggattttca atcccctgct ctaccgactg agctatccgg
     5641 gcaacggggc gcattaaacc tgattcgcct cgtctcgtca atcaaatttc ttcaatttac
     5701 tgcagactgc acaatctatc atcactttgc cgctattgca cgcattgtca ggcaaaccag
     5761 gcggtccagg cggcggaaag cggcattgcc agaaggagtg ctggaagcat gttgaccacc
     5821 gggaacattt taatgccaca gatgcgtaat ccggttgcca gcagcaataa accgcctacg
     5881 gcgctgaagt ctgccatcat cgacggtgtg atcagcggta atatcagcgc ggcagcccac
     5941 gccagcgtta actggatgat cagtaatggg atactaatta ccgacaccgc aatacccagc
     6001 gagcaggcga agatcatcgc cgtaaagaaa tcaagaaatg acttggcgat taaaatactc
     6061 ggatcgccgg tcatcccttc gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac
     6121 aggacaataa tcgcgacata attctgaata aaagattcat gcgctggctt cttacgtgag
     6181 tgacgaaaca gattttgtgc tttggcgact gctgtattga cacctttctc cagcaaacaa
     6241 atttcgccaa ttagagcgcc aagtagcgta gctaaaacca tcgctggaag gttggcgcat
     6301 ttcacaacca gtaaaatacc aatccccagc gatgccagac caaaaataga tgtcatggag
     6361 acgcggatac gttccggtaa gcgttggctg agcagtgcgc cgagaacgcc acccagtaag
     6421 actgcactag cgttgataaa agggccgatg accacaagag ttcctgttag ctgtattgtt
     6481 tgattttctt attatgaact ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg
     6541 cttgcacgcc ttagcgaaag gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt
     6601 ttgcccgatg cacgccatct ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt
     6661 ttccctttct atgttactgc tcatgcggtg aagttaacgc acgctcactg caggacaaca
     6721 gtaaaatcag agcgtttctg cttttactga tgtctggcgg tcggagctgg tgaccagttt
     6781 gacccatatc tcatggggca gggttttcca ccttgtccgg tattcttact tccccgaaac
     6841 gggtttgcgc ttatgaaatc aatgaatatt gccgccagta gtgaactggt atcccgactt
     6901 tcttctcatc gccgcgtggt ggcgttggga gatactgatt ttacggacgt cgcggcagtc
     6961 gtcattaccg ctgcggatag tcgcagtggc attcttgcgt tgcttaagcg caccggtttt
     7021 catctaccgg tgtttttgta ttccgaacat gctgttgaat tacctgcggg cgttacggcg
     7081 gtaatcaacg gcaacgagca gcagtggctg gagctggaat ccgcagcctg tcagtatgaa
     7141 gagaatttgc tgccaccgtt ttatgacacg ctgacgcagt acgttgagat gggcaacagc
     7201 acctttgctt gccctggaca tcaacatggt gcgttcttta aaaagcatcc tgccggacgc
     7261 catttttacg atttctttgg tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta
     7321 aaattgggcg atctgcttat tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc
     7381 aaagtctttc atgccgataa aacctatttt gtgctgaacg gcacatcggc agcgaataaa
     7441 gtggtgacga atgcgctgtt aacgcgtggc gatctggtgc tcttcgaccg taacaaccat
     7501 aagtcgaatc atcacggcgc gctgattcag gcggggacga cgccggtcta tctggaagct
     7561 tcacgtaacc cgtttggttt cattggcggt attgatgcgc actgttttaa tgaagagtat
     7621 ctgcgccagc aaattcgcga cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc
     7681 ctgacgatta ttcagctggg aacctatgac ggcactgtct ataacgcccg tcaggtgatc
     7741 gataccattg ggcatctgtg tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa
     7801 cagtttatct cgatgatggc ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat
     7861 ccggggattt ttgtgaccca gttggtgcac aaacagcagg ctggattctc acagacgtcg
     7921 cagatccata aaaaagataa ccatatccgc ggacaggcgc gtttttgccc gcataagcgg
     7981 ttgaataacg cctttatgct ccatgcttct accagccctt tctatccgct gtttgctgca
     8041 ctggatgtta acgccaaaat tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc
     8101 gttgagttgg gtattgagtc gcgcaaggct attcttgcgc gctgtaagct gttccgcccg
     8161 tttatcccgc ccgttgttga tggcaaattg tggcaggatt atccgacatc agtgttagcc
     8221 agcgaccgcc gttttttcag ttttgagccg ggggcgaagt ggcacggctt tgaaggatat
     8281 gccgcggatc agtattttgt tgatccgtgc aagctgttac tcactacacc aggtatcaat
     8341 gccgaaaccg gcgaatatag cgactttggc gttccggcga cgattctggc gcactatctg
     8401 cgtgagaacg gcattgtgcc ggagaagtgc gatctcaact ccattctgtt cttattaact
     8461 ccggcggaaa gccacgagaa gctggcacaa ctggtggcga tgctggcgca atttgaacag
     8521 catattgagg atgactcgtc gctggctgag gtgttgccga gcgtttataa caaatatccg
     8581 gtgcgctatc gcgactacac cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc
     8641 agtttcgacg tcaaagacct acaaaaagcg atgttccgcc agcagagttt cccgtcagtg
     8701 gtgatgaacc cccaggatgc gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg
     8761 attcgtgatg ccgaagggcg aattgcggca gaaggggcgt tgccttatcc acctggcgtg
     8821 ctttgcgtgg tacccgggga agtctggggt ggggcggttc aacgttattt ccttgcgctg
     8881 gaagaaggcg tgaatttgtt gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa
     8941 accgatgcgg atggcatgaa acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac
     9001 gggtcacctt ctggcgaccc gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc
     9061 tgtcgggaca cgaacgtgtt tatatttgaa catcgccatg aacgcgaagg ccagaaccac
     9121 ggaataacca gcgaaaatca accatacggt ctgccagtcg gtaatgccgt tttgggtgta
     9181 catctcaaca actttaccgc tcacgatgcc gccgaggata cagccgaagc cgttagtcat
     9241 catcaggaac atcccttgtg cactggcgcg aattgccggg ctaacttctt tttcgacaaa
     9301 caccgaacca gagatgttga agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac
     9361 cagcagtaca gtaccgaacg gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat
     9421 ccacgccaca atactgatca tcattacgtt cttaataccg tagcggctta agaagaacgg
     9481 gatggtcaga atgaacaggg tttcagagat ctgcgaaatc gacatgatga ttgacgcatg
     9541 ctgcacgatg aagctgctgg caaacatcgg atctttgtcg aagctgtgca ggaaggtatt
     9601 accgaacatg ttggtaatct gcagttccgc gcccagcagc attgagaaga tgaagaagat
     9661 tgccatacgc ttgtttttaa acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct
     9721 ctgattcgct tgctgtttag caaccggaat atgcggcaga gtcagggtaa acagaaccag
     9781 aatggcggaa agtgctgcgc caatatacag ctgcatgtgg cttaattcga agccagacag
     9841 gctcaccacc cacattgcca tgataaagcc gatggtgccc cagatacgga ttggcgggaa
     9901 gtcagtaacg atatccatcc cggcattttg caggcgatag taagagatgg tgttgattaa
     9961 cccaagcgtt ggcatataag caaacgagtt aatcaatatc acaaggaaca tcgcttccgg
    10021 cgtcgtgacc tgtgccgcca tgaacagcgt gatagcgcca atggtgtggc aaatggcata
    10081 tacccatttc gcacttaacc atttgtcggc cacaatcccc agcagcgcag gcataaatac
    10141 cgcggcgata cccagtgagc tataaactgc gccaatagaa gcaccgtcaa acttcagggt
    10201 aacaaacata taggagccga gggtcgtcag ccaacttccc cacagacaga actgcagaaa
    10261 agagaggatt ttcagctgca gcttaagatt catgttaatt tcctcacatc gtgatgcgga
    10321 tgagtgtttg aaaaagcaca tttgaccgtg cgagatgatg ggattctatc aacttcgcgg
    10381 actggttttt tgttacctgt ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat
    10441 tgtgatgtgg ataacatttt tgcccctgag catcgtcagg ggcggttaat ggaattatcg
    10501 gcggcggtag gatttttgcg cggtattcac tttataaaga taacggcgag attccgcaga
    10561 gggatggcgg gtcgtcagcg tctgataaac atcgcccggc gtcatggtgt taataatatt
    10621 ggcagcctga atcttatcat tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata
    10681 ggcggtgatg acggcataac gccgcgatgt tgggttatca attccgccga gataaacatt
    10741 gttcagcatc gccagatacg cggtgccggt atcaatattg ctggcaggat caaacaagaa
    10801 actgcggctc ggcgtgccgg atttcccctg cgagcggaac acatctttcc cggcagtatt
    10861 ttgtaccacc tgcattaatc ccagcgcatc ggaacggctg accgcatacg ggttaaagga
    10921 agattcagtc tgcataattg ccagaatcag cgactcatca acgccatatt tccgtgacgc
    10981 ctggcggacc atgccgagat atttgtgcgc acgtttatca aggtggttcg gcaccatgtt
    11041 aatggtgacg ctgtagatga tacgtagccc gttgctgcgg cttttcagac ggtttttcag
    11101 cagataatcc gcgaagttgc ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc
    11161 caccacctga ccgtaaagga aaggttcttt cgaaattgta atatcatcaa cgtcggaata
    11221 gagatcgacc gaactcggat cgtcacccat cagcaacgtt ttgataattg cccggcgcaa
    11281 atgcgcggca ggttctgtcc cggcgatggt ttcgatagta atcgtaccgt catcgaagtt
    11341 gatgtggctg cgggtctgat attgatcggt gtatttcacg tagtccttag gaccggcgat
    11401 caccacctct ttgaagcccc agatgttctc aatattgtgg gcaaattgcc ccatcagaat
    11461 atcaaaaccg ttggtatctt tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt
    11521 cgaacaggag atgagcaacg gcgcaatcaa agccagcgcg agatattttt tcatcattcc
    11581 gggtgcgtgt tgtgtttgtt tttatgcaag ctgccggagg cgtgctccgg cactgttttt
    11641 attttttatc ttccggcgta tagccctcga tatgcacctc tttaccctcg aacaggaagt
    11701 tgaccatctc ctgctccagc agcttgcggt gctcggcatt catcatgttg agtttctttt
    11761 cattaatcag catggtttgt ttgtgctgcc actgcgccca ggcttctttg gagatctcgt
    11821 tatagatgcg ttttcccagc tcgccggggt acagctgaaa atcctgacct tctgcttcac
    11881 gttgcaggaa agtacaaaaa atcgttctgc tcataaatca tcctctttat cgactcacgc
    11941 gctaaaccgg cgcgccagtg cgtaactgct gtaacaaacg ctccacggga gccgccaggc
    12001 caactgacgg cggttgcgct aagttatacc agagcgcatt gccttcatcc atgcagccgg
    12061 tgaatgacga cacgggaagc cacataggca caatatctaa gtggaaatgg ctgaaggtat
    12121 ggcgaaacgc ggtcagttgc gtcaggttat cggcagcaat ctgccgttgt gccagccaat
    12181 gccgcaaact ttcttcgtcg gcaaactgcg ggaaacagta taaaccgccc cacaatccgc
    12241 tcggcggacg ctgcgccagc aatacttcat cttcgtgctg taacaggaga aagtagccgg
    12301 tgcgctccgg cagcgtctgt ttcggttttt tgcccggata aagcgaccag ctattgttgg
    12361 tggcggcaat acatccgttt tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc
    12421 aaatcatcgc gcccaaatcc atcatcgcct gattaaaccg ttccacgccg accgcgggcg
    12481 tcacctgctc gctcaaactc catagtttat tctcgacctc ttttttccca ggccagccgc
    12541 ttacagcata gcagcgcgcc agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct
    12601 tacccagaga aagcgagaga atcgcgcctg cggtggaacg cccgacgccc ggtaacgccg
    12661 cgacttcttc aaaggtttcc gggaatttac caccgtgtaa ggtcgccact tgttgtgccg
    12721 ctttatgcag gttgcgcgcg cgggcgtaat agccaagccc ggtccacaag tggagaactt
    12781 cgtcgagcgg cgcattggcg agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa
    12841 aataggggat aacggtcgca acctgagttt gttgcaacat cacttctgag agccatactt
    12901 tgtagggcgt cttgtcaatt tgccagggca gcgttttccg cccgtattta tcgtaccagt
    12961 ccagaacctg ggctgaaaat tgcgacgctt gcatggtcac cgacttcact gttgttgggg
    13021 gcaagattgc agcacagcga cggcagggtg taaaccggaa ctttccgcag ccacggcctt
    13081 atcatgcttg catccggcaa ctaactttgg ataatgcccg ttttcagaac actttcacaa
    13141 gcgactaaat ctttatgaaa aacgacgtca tttcaccgga atttgatgaa aacggccgcc
    13201 cactgcgccg tatccgtagt tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac
    13261 atgcgctgga aaactactgg ccggtgatgg gcgttgagtt cagtgaagat atgctggatt
    13321 tccccgcgct ttttggccgt gaagcgccgg tgacgcttga gattggtttt ggcatggggg
    13381 cgtcgctggt ggcaatggct aaagatcgcc ccgagcagga cttcctcggc attgaagtgc
    13441 attcaccggg cgttggtgcg tgcctggctt ctgcgcatga agaaggttta agcaacctgc
    13501 gcgtgatgtg tcacgatgcg gttgaagtgc tgcataaaat gattcctgac aattcattgc
    13561 gcatggtgca gctctttttc cctgacccgt ggcacaaagc gcgccataat aaacgccgta
    13621 tcgttcaggt gccgtttgcc gaactggtaa aaagcaaact gcagctgggg ggcgtattcc
    13681 atatggcgac cgactgggaa ccttatgcgg aacatatgct tgaagtgatg tcttctattg
    13741 acggttataa aaacctgtca gagagcaatg attacgtacc gcgtccggca tcacgtccgg
    13801 tgacgaaatt tgaacaacgt ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg
    13861 agagggtgaa ataatggcaa agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga
    13921 cgaattccag gaattaggat tttcggtggc atggcgattc ccggaaggta catcggaaga
    13981 acagattgat aaaatcgttg atgactttat taacgaggtt atcgaaccga acaaactggc
    14041 ctttgacggc agcggttatc tggcctggga aggtctgatc tgcatgcagg aaatcggcaa
    14101 atgcaccgaa gaacatcagg cgattgtgcg taagtggctg gaagagtgca aactggatga
    14161 ggtacgcacc agcgaacttt tcgacgtttg gtgggactaa gaaagcatac gggcgatgac
    14221 aaatgcaaaa ctgcctgatg cgctacgctt atcaggccta cagagatgca cgatcgagta
    14281 ggcgggataa ggcgtttacg ccgcatccgg catggaaaac gcgtactttg ttatcaatct
    14341 ggggccagca tatgctggcc tgatttgttc ttgagggaag actatgatgc gcaaaatgct
    14401 gctggcggca gcactttcag tgacggcaat gaccgctcac gccgactacc agtgcagcgt
    14461 cacgccgcgt gacgatgtga ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg
    14521 caatctggtg atcacgccag acggcaacgt gatgtataac ggtaagcaat attccctgaa
    14581 tgccgcccag cgcgagcagg cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg
    14641 gattgatgaa ggcgcgaaaa gccgcgtcga gaaagcccgt attgcgctgg ataaaattat
    14701 cgttcaggag atgggcgaca gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca
    14761 gcttaaagag cagatgaatc gcattatcga aacgcgcagc gatggcctga cgttccacta
    14821 taaagccatt gatcaggttc gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg
    14881 aattttacag gacagcatta atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa
    14941 cccattacag aatgtgctgg gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg
    15001 gaaaaagcag gaaaaagatt tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac
    15061 tctggaagat agccgcaaag ccctggtcgg gaatttaaaa taatcctcta ttttaagacg
    15121 gcataatact tttttatgcc gtttaattct tcgttttgtt acctgcctct aactttgtag
    15181 atctccaaaa tatattcacg ttgtaaattg tttaacgtca aatttcccat gcaaagctaa
    15241 gggataatgc gtagcgtcca cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg
    15301 cacttgccgc actggttatg ggttttagtg gtgcagcatt ggcattaccc aatatcacca
    15361 ttttagcaac cggcgggacc attgccggtg gtggtgactc cgcaaccaaa tctaactaca
    15421 cagcgggtaa agttggcgta gaaaatctgg ttaatgcggt gccgcaacta aaagacattg
    15481 cgaacgttaa aggcgagcag gtagtgaata tcggctccca ggacatgaac gataatgtct
    15541 ggctgacact ggcgaaaaaa attaacgccg actgcgataa aaccgacggc ttcgtcatta
    15601 cccacggtac cgacacgatg gaagaaactg cttacttcct cgacctgacg gtgaaatgcg
    15661 acaaaccggt ggtgatggtc ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc
    15721 cattcaacct gtataacgcg gtagtgaccg cagctgataa agcctccgct aatcgtggcg
    15781 tgctggtggt gatgaacgac accgtactgg acggtcgcga tgtcaccaaa accaacacca
    15841 ccgacgtagc gaccttcaag tctgttaact acggtcctct gggatacatt cacaacggta
    15901 agattgatta ccaacgtacc ccggcacgta agcacaccag cgatacgcca ttcgatgtct
    15961 ctaagctgaa tgagctgccg aaagtcggca tcgtttataa ctacgctaac gcatccgatc
    16021 ttccggctaa agcactggta gatgcgggct atgatggcat cgttagcgct ggtgtgggta
    16081 atggtaacct gtataaatcc gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg
    16141 cagtagtgcg ttcttcccgc gtaccgacgg gtgctaccac tcaggatgct gaagtggatg
    16201 atgcgaaata cggcttcgtc gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc
    16261 tgcagctggc tctgacgcaa accaaagatc cgcagcagat ccagcagatt ttcaatcagt
    16321 actaatcacc tcgtcccggc atcgtgccgg ggctttttca cttcagactt acgtccattg
    16381 ccaattttca ttaccctaat gataatcacc ggaataaatt attccgcgcg agggttttcg
    16441 ggtgaaaaag caatggattg ttggcacggc gctgcttatg ttgatgactg gtaatgcctg
    16501 ggcagatggc gaaccgccaa ctgaaaatat cttaaaagat caattcaaaa agcagtatca
    16561 cggcattctc aagcttgatg ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc
    16621 cacctggtca gcggaaggcg atgtctcttc cagtgacgat ctctatacct gggtcggtca
    16681 gttggcagat tacgaactgc tcgaacagac ctggacgaaa gataaaccgg taaaattctc
    16741 ggcgatgtta accagtaaag gaacgcccgc gtctggctgg tcggtgaact tttactcttt
    16801 tcaggcggca gccagcgatc gtgggcgggt ggtcgacgat atcaaaacga ataataaata
    16861 tctgatcgtg aatagcgaag atttcaatta tcgctttagt cagcttgaat ctgcgttgaa
    16921 tacccagaag aactcgattc ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat
    16981 ggtggcggcg cagaaagcag cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat
    17041 gacccgcgaa gaagccttta aaaaaattca ccaacagcgt gatgagttta ataaacagaa
    17101 cgatagcgag gcgttcgcgg ttaaatatga caaagaggtt tatcaaccgg cgattgcggc
    17161 atgtcataaa cagagtgaag agtgttatga agtgccgatt cagcagaaac aagatttcga
    17221 tatcaacgaa caacggcgac agacttttct gcaatcacaa aaactcagcc gtaaattgca
    17281 ggatgactgg gtaacgctcg aaaaagggca atatccgtta acgatgaaag tttcagaaat
    17341 aaacagtaaa aaagtcgcga ttttgatgaa aattgacgat atcaaccaag ctaatgaacg
    17401 ctagaaaaaa gataccgaac aactgcggcg caatggggtg attaagtaac tcatgttaag
    17461 tgcctttgcc ggataatata aaaagttatc cggcaaatta ttttttacat tagccctagt
    17521 agctgtggaa tgaacaagga tatttgcgga atgtaagtga tcatcatcaa cgctactagc
    17581 aatgcagcat aaaatagcag caatggtttg attaaatgtt gaatcttcac acccgagata
    17641 gaacaaccaa caaatagcgc actacccacc ggcggtgtta acagcccaat acaaaggttg
    17701 gccaccatca taataccgaa atgtactggg tccattccca gctcttgtgc aatcggcaag
    17761 aagatcggtg taaagattag tacagccggt gtcatgtcca taaagatgcc gacaattaat
    17821 aagacgatat taatcagcag caatatgatc agtgggttat cagaaatccc catcaatgcg
    17881 tcgctgatca tataaggaat atcagcatta gtcattgccc aggacatgcc cacggaaaaa
    17941 ccgatcaaca gtaatacaat cgaagtggta acaaccgact ccagaatcag tttgggtaaa
    18001 tggcgccatt ttacttcccg gtaaatcagc accgagagaa taaatgtgta gacaacggca
    18061 attgctgacg cttcagttgc ggtaaaaatg ccgcccagaa tgccgcccat cacaatgaac
    18121 accagaagca aacttggtaa ggcatccaga aacgctttac aggcctgagc aaaggttgct
    18181 ctttctgaaa gcgggtagcc acggcgttta gcaataatgc cgcagactat cattaccgca
    18241 agacccatta agatccctgg caaatatccc gccataaaca gcgatgctac agaaacgccg
    18301 ccagctgtca gggaaaacac aattaacacg tttgagggcg gtatcagtaa tccagtaata
    18361 caagatgaga cgtttacagc ggtagaaaat gccggatcat aaccttcttt ggtctgaatg
    18421 ggattcagtg tgccaccaac ggcagcagct gccgcaacgg ctgaaccaga aatggaacca
    18481 aacatcatat tcgccaggac gttcacatga ccaagcgaac caggaacgcg gccaaccatt
    18541 acctgggcga ggttaatcaa acgtatcgca ataccgccgc tgttcatcag tgttccggca
    18601 aagatgaaaa aggggattgc caacagggca aagttatcca gtccattagc cagtcgttgc
    18661 gaaacagtga ttgccgcgat atcccaggga aacatcaaaa gcattgaagc tacagtggca
    18721 ataccgattg agaacgaaat gggcacacca ataaagacca gcacaaaaaa acagccaaac
    18781 agtgtaatcg cgatataact atc
//
LOCUS       DAJTSI010000079        18474 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000079 DAJTSI010000000
VERSION     DAJTSI010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.079"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            11..706
                     /locus_tag="OO327_002424"
     CDS             11..706
                     /locus_tag="OO327_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR6018937.1"
                     /translation="MNSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRS
                     LDINPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGL
                     TLGIGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTF
                     SARILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETA
                     DTEPFAVWLEKHA"
     gene            856..1371
                     /gene="luxS"
                     /locus_tag="OO327_002425"
     CDS             856..1371
                     /gene="luxS"
                     /locus_tag="OO327_002425"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR6018938.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(1435..2973)
                     /gene="emrB"
                     /locus_tag="OO327_002426"
     CDS             complement(1435..2973)
                     /gene="emrB"
                     /locus_tag="OO327_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR6018939.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(2990..4162)
                     /gene="emrA"
                     /locus_tag="OO327_002427"
                     /pseudo
     CDS             complement(2990..4162)
                     /gene="emrA"
                     /locus_tag="OO327_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            complement(4289..4819)
                     /gene="emrR"
                     /locus_tag="OO327_002428"
     CDS             complement(4289..4819)
                     /gene="emrR"
                     /locus_tag="OO327_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR6018940.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(4910..5245)
                     /gene="ygaH"
                     /locus_tag="OO327_002429"
     CDS             complement(4910..5245)
                     /gene="ygaH"
                     /locus_tag="OO327_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR6018941.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(5235..5972)
                     /gene="ygaZ"
                     /locus_tag="OO327_002430"
     CDS             complement(5235..5972)
                     /gene="ygaZ"
                     /locus_tag="OO327_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR6018942.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(6096..7275)
                     /locus_tag="OO327_002431"
                     /pseudo
     CDS             complement(6096..7275)
                     /locus_tag="OO327_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(7467..8459)
                     /gene="proX"
                     /locus_tag="OO327_002432"
     CDS             complement(7467..8459)
                     /gene="proX"
                     /locus_tag="OO327_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR6018943.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(8517..9581)
                     /gene="proW"
                     /locus_tag="OO327_002433"
     CDS             complement(8517..9581)
                     /gene="proW"
                     /locus_tag="OO327_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR6018944.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(9574..10776)
                     /gene="proV"
                     /locus_tag="OO327_002434"
     CDS             complement(9574..10776)
                     /gene="proV"
                     /locus_tag="OO327_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR6018945.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(11130..12089)
                     /gene="nrdF"
                     /locus_tag="OO327_002435"
     CDS             complement(11130..12089)
                     /gene="nrdF"
                     /locus_tag="OO327_002435"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR6018946.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(12099..14243)
                     /gene="nrdE"
                     /locus_tag="OO327_002436"
     CDS             complement(12099..14243)
                     /gene="nrdE"
                     /locus_tag="OO327_002436"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR6018947.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDDTLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(14216..14626)
                     /gene="nrdI"
                     /locus_tag="OO327_002437"
     CDS             complement(14216..14626)
                     /gene="nrdI"
                     /locus_tag="OO327_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR6018948.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(14623..14868)
                     /gene="nrdH"
                     /locus_tag="OO327_002438"
     CDS             complement(14623..14868)
                     /gene="nrdH"
                     /locus_tag="OO327_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR6018949.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(15215..15445)
                     /locus_tag="OO327_002439"
     CDS             complement(15215..15445)
                     /locus_tag="OO327_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018950.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            15597..15941
                     /locus_tag="OO327_002440"
     CDS             15597..15941
                     /locus_tag="OO327_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR6018951.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(15978..16427)
                     /gene="alaE"
                     /locus_tag="OO327_002441"
     CDS             complement(15978..16427)
                     /gene="alaE"
                     /locus_tag="OO327_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR6018952.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            17095..17499
                     /gene="stpA"
                     /locus_tag="OO327_002442"
     CDS             17095..17499
                     /gene="stpA"
                     /locus_tag="OO327_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR6018953.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(17546..18070)
                     /gene="ygaP"
                     /locus_tag="OO327_002443"
     CDS             complement(17546..18070)
                     /gene="ygaP"
                     /locus_tag="OO327_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR6018954.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(18080..18379)
                     /locus_tag="OO327_002444"
     CDS             complement(18080..18379)
                     /locus_tag="OO327_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6018955.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT         4709 a         4654 c         4712 g         4399 t
ORIGIN      
        1 gaggctgcaa atcaatagca acgtgctgca gattgaaaac gaactgtatg cgccgattcg
       61 tccaaaacgc gttacccgca gcggcgagtc accttctgat gcgctgttac gtggcggcat
      121 tgaatatatt gaagtgcgtt cgctggacat caacccgttc tcgccgattg gtgtagatga
      181 acagcaggtg cgattcctcg acctgtttat ggtctggtgt gcgctggctg atgcaccgga
      241 aatgagcagt agcgaacttg cctgtacgcg cgttaactgg aaccgggtga tccttgaagg
      301 tcgcaaaccg ggcctgacgc tgggtatcgg ctgcgaaacc gcgcagttcc cgttactgca
      361 ggtgggtaaa gatctgttcc gcgatctgaa acgcgtcgcg caaacgctgg atagcattaa
      421 cggcggcgaa gcgtatcaga aagtgtgtga tgaactggtt gcctgcttcg ataatcccga
      481 tctgactttc tctgcccgta tcttaaggtc tatgattgat actggtattg gcggaacagg
      541 caaagcgttt gcagaagcct accgtaatct gctgcgtgaa gagccgctgg aaattctgcg
      601 cgaagaggat tttgtagccg agcgcgaggc atctgaacgc cgtcagcagg aaatggaaac
      661 cgctgatacc gaaccgtttg cggtgtggct ggaaaaacac gcctgacaga aaagaaaaag
      721 gccactcggg agtggccaaa atttcatctc tgaattcagg gatgatgata acaaatgcgc
      781 gtctttcata tactcagact cgcctgggaa gaaagagttc agaaaatttt taaaaaaatt
      841 accggaggtg gctaaatgcc gttgttagat agcttcacag tcgatcatac ccggatggaa
      901 gcgcctgcag ttcgggtggc gaaaacaatg aacaccccgc atggcgacgc aatcaccgtg
      961 ttcgatctgc gcttctgcgt gccgaacaaa gaagtgatgc cagaaagagg gatccatacc
     1021 ctggagcacc tgtttgctgg ttttatgcgt aaccatctta acggtaatgg tgtagagatt
     1081 atcgatatct cgccaatggg ctgccgcacc ggtttttata tgagtctgat tggtacgcca
     1141 gatgagcagc gtgttgctga tgcctggaaa gcggcaatgg aagacgtgct gaaagtgcag
     1201 gatcagaatc agatcccgga actgaacgtc taccagtgtg gcacttacca gatgcactcg
     1261 ttgcaggaag cgcaggatat tgcgcgtagc attctggaac gtgaggtacg catcaacagc
     1321 aacgaagaac tggcactgcc gaaagagaag ttgcaggaac tgcacatcta gtcagtaaac
     1381 tatcttcaca attaattgaa aaaagccagt tcaaatgaac tggcttagtt gtacttagtg
     1441 cgcaccgcct ccgccgccac ctgcgccaaa tggcggttta gcaaaccaca ccagccccag
     1501 cagtacgagg aatatcccgg ctgacatcca gaagatctca ttggcggaaa taatcagccc
     1561 ctgattggtg atctgctggg caatccagcc tgatgcctgc tgttgcgtca tcccaagccc
     1621 ttccagttga ctgtacatcg cctgggcatt cgggttgaac gggtttaccg actcagtcaa
     1681 ctgcgcatgg tgcatcgact cgcggttggt ccacatggtc gtggttatcg acgtgccgat
     1741 agaccccgcc agcgttcgcg taaagttaga gaggctcgat gccgccgcca gtcgttccgg
     1801 tggcaaacca gacagcgtaa tggtggtcag cggcataaag aagcagacca ccgcaaaccc
     1861 ctggataaac tgcggccagg ccgacgcgca aaaatccata cctggttcaa aggtataggc
     1921 acgccagtag aagcagacgg catacataat aaagctgaag gttaccagcc gccgcatatc
     1981 cagtttatgc gcgaagcggc cgataatcgg cgacagaatc accggaataa tccctaccgg
     2041 cgcagaggcc aaacctgccc aggtcgccgt gtaaccgtag acctcctgca acaactgcgg
     2101 cagcagaaca atagcgccga agtagagcat atacgcgagg ctgatacaca agcagccgat
     2161 ggtgaagttg cgcgacttaa acaacgacag atcgactatc gggttatcgt cggtcagctc
     2221 ccagacaatc aggaagcaga tagccaccac cgccaccacg gtaagaatga taatttcctg
     2281 tgatgaaaaa cagtccagct ctttaccgcg gtcgagcata atctgcaggc tgccgatacc
     2341 aataaccagc agtgccagcc ccacggcatc aatccgccgc cgttcggtgc gggtttcacg
     2401 tccgcgcagg gtttgcagtg tcatcaacac caccgccacg ccaatcggca cgttgatgaa
     2461 gaatatccag ccccagtggt aattatcgct gatataaccg ccgaggatcg ggccgcaaat
     2521 tggcgcgaca atcaccgtca tcgaccacaa cgccagcgcg atcgagcgtt tggctggcgg
     2581 gtagttattc agcaatagac tttgcgaaag cgggatcaac ggcccggcga caatcccctg
     2641 aatcacgcgg aagaagatca gcatattcag gctgctggag acaccacacg cccaagacgc
     2701 aatagcaaag gcgatggtgg accaaaggaa cagtttcact tccccgacgc gctttgccag
     2761 ccagccggta agcgggatcg agatggcatt cgccaccccg aaagaagtga ttacccacgt
     2821 tccttggctg agcgatgagc ccagattccc ggcgatagtg gggatcgcca cgttagcaat
     2881 ggtggagttc agcacctgca tgaatgtcgc cagtgacagc gcaatcgtca taatgaccag
     2941 ttgcgcgcct tccagcggtt tttgctgttg catcacacgc acctctggat tagccagcgt
     3001 tagcttttac gatatcgtcg atcagtttat tgacaggtgc caggctgatt tcacgcgcgg
     3061 tacttaccgc gaccggtgtt gaacgtactt tatttgccag tacctgaccg tcacggttag
     3121 tggtattgac gctcaccagc gtggacaaac caatgcgcag cgggtattgc tccagctgtt
     3181 tctggtccag ttcgatacgc acaggtagac gctgaacgac tttgatccag ttaccggtcg
     3241 cattttgcgc cggaagcagt gagaacgcgc tacctgtgcc catatccaga ccaaccactt
     3301 taccggtgta tttcacatca tcgccgtaaa tatcggtggt gatggtgacc ggctgaccga
     3361 tgcgcatatt ggcaatctgc gtctctttaa agttggcatc cacccacata ttggtggctg
     3421 gaacgaccgc catcagcggc gtcgttgggc taatttgcgc acccggttgc acggcacgac
     3481 gggaaacata accggtcatc ggactgacaa tacgggtacg ctccagcgcc agctaggcgt
     3541 tacgtacttc ggtggcagcc tgttgcacgg caggctgatc ttccagttta gtccccagaa
     3601 tcatcgcctg attggcattg tattgttgaa tagcggcgtc cagttgcgcc tgggcactgg
     3661 tgacggcgtc gcgggcgtgt tgcagctctt cgcgaccaat caggttggca ttgcccagcg
     3721 gcacacggcg gttgtagtcg ctttgtgctt tcgcgagggc gattttttgc acctcaatat
     3781 tcgcctgcaa ctgtttgctg ttaatcatca gctggtgggt ttggcgaacg ctggaagcca
     3841 gtgcagtttt ggctttttca aacgcctggc gagcatctgt cgggtcgaga gtgaccagaa
     3901 catcgccttc ttttacaaaa tcggtgttat cggcccagac tttcgtcacg ctgccagaca
     3961 cctgagacat aatttgaatc tgattccctg ccacgtatgc gtcatcggtt tcttcgaaat
     4021 gacgcagtac caaaaaccaa taaatcccta tcgctacggc aataattata aagagcaagg
     4081 tgagaaggag gagcagacgc ttacgtttgc cgctcttctt taccggttgc tgcggggttt
     4141 gagtctccgc atttgcgctc atattgttct ccacgatctt cttaactcat cggctgagcc
     4201 gacctgcttg ccaaaaaggc cagcatgttg cgatgctggc cggtcatttt ttcttttata
     4261 aatctggatt tttgagcgag atgacgcgtt agctcatcgc ttcgagaacc acaccgtctt
     4321 gttccatctg gtcgagacgg gagagcaatt tgcgggtgat ttgctcgagc tgatcttttt
     4381 ctgttgtgct gagcgcggac cagagttgat gcaggcagtt atgctgcggt ggtaaaacct
     4441 cgcgcaaaaa ctcgtgacct ttttccgtta attgcagatg caggcagcgg cgatcgttat
     4501 cgcttttacg acgttcgatc caaccgcgtt tttccagttc atcggcaata cgcgtcgcgt
     4561 tggtacggga tgatccaaga gcacaactta attcagaagg ctgaatactg tggttttcct
     4621 gagactccag cgtaatcaac gccataaaca acgtctcgtt aatcccctga gccttcagca
     4681 ttttattgcg gttctccagc agcttgcttt gcatgtgcat gcaaagacga gtcagaagga
     4741 tctcctgata aggaaaatct tcgtggcggc tggcgcgaaa ttttagcatt tgttcaatgg
     4801 gcgtaaacga actatccatt tgggtatgac ctcattaatt tcagccgata tagtaacgac
     4861 agtgacaaat aaagtaaatg tattgtttta gaaaaatgat tcttgtgggt tatataatcg
     4921 ccatcacttt ccaggcgagc ccataggcca gcgcactaag cagtgttggg atgataatgc
     4981 tgcgtgtttt atagaaactg gcacccagta ccgcgaagcc gaccagcgtg ggcacgaaac
     5041 ggcgtgtatc gtgcatcact tctggtgcgg tagagacaac cagcagagcg catatcgagg
     5101 cgatgccaat ggtgtcgagc aaaataccta ccgcgccacg tttggttggg cgggcattac
     5161 ccacacgcag gcgcagcggc aaatagcgga agcaataatt cgccgcgcca accagtaacc
     5221 caagcagcag aacctcatag ctcatcgggc gctccttgcc agaatgcctg gattaacgca
     5281 gtgaggcagc cacagacaat gcctgccaga atggcgacgg gaatagaaaa tagcgttacg
     5341 cctgcaaggg caccaactaa agctgcggta acgcaaagag attgtttgcg ctggaaagag
     5401 gcgagcagga aactcataaa gagtgccgga agcataaaac ctaatgcagc ttcaacggcg
     5461 ggataacctt gcagcaagcc gctgccggag aatgccccta ttaccgtacc aaatacccac
     5521 gatgaccatg aactgaaggc aatgccgatc atccagttct cgctccagcg gcgattattg
     5581 cgtaccagtt ttgcggtggc ggcggcaaaa acctcatccg tcaggccaaa cgcccatagg
     5641 gcggttttcg atttttgcag acgctgaata atacggctac gcagtgacgg gccatacaaa
     5701 acatggcgaa catccattgc catgacgctc agtgcagcaa cccacaaact actcccggct
     5761 gccagcatcg cggtaatgac gaactggctc gcgcctgcat aaatgatgca ggagaaaaaa
     5821 acgctttcga gaggagagaa tcccagacgg gtcgcattca gaccgaacgc aaaggccacc
     5881 ggaatataac taataacaat cggtaaactg tctttgcatc cttccatgaa ggtcgccgaa
     5941 ccaggagcag gctgtggagt agggctttcc atagagtgta cgcttaacga ttgttaatat
     6001 tataaataga ctgaatgaat atcttaacct tatcagactg atgggcttct taacaccctt
     6061 ataagtgtaa agccacgaaa acggttgctg attgcttaat ttgcggcttc ctgacgatga
     6121 aaacctcgcc accagaccag taagttaacc agggcaatcg tcgcgccagc cagacaaacg
     6181 ccagcccaac cgccatgttg ccaggctgag gctgaaatta gcgaaccggc ggcaccgcca
     6241 ataaagtagc tggtcatgta acctgcggtc aggcgattac gcgcatcagg atgtattcga
     6301 taaattaccg tctggttagt gatatgcacg ccctgtacgg tgaggtccag caccagaatg
     6361 ccgataatca acgccagtac ggaagtgtga ccaaaccaga ttgccagcca tgaaagtaac
     6421 agcagaagca gaccgaaagt tgtggtgcga tttgcccttg tcggcaaaac cgcccgccgg
     6481 acgagcgccc aacgctccgg cagctcccgc aagcccaaac agaccaatgt caccatcgct
     6541 gtagttaaaa ggtggagcgg caagcaaaaa ggccattgag gtccagagaa tgctgaagtt
     6601 ggcaaaggtc atgcagccca gcaacgcgcg ggtgcgcagg atcttatcgc tgatgaacat
     6661 gctgaaaacg gaacccaaca actgtgggta gttgaggtgg gtttctgatt tcatttgtgg
     6721 cagaccacgc cataatgcca gcgccatcag tgccattaac accgaagcaa cccaaaagac
     6781 ggtgcgccag ccgccgaggt ttgccagtaa cccggcaacg gttcgtgcca gcaatatccc
     6841 caacagcaga ccgctcataa tggtgccaac cactttgccg cgtttatccg gtgaagccag
     6901 cgtcgctgcc agcggaacca gaatttgtgc cacgactgag aataaaccgg ttaatgccgt
     6961 accgaggatc atcatcgcca gtgactgact gctggcagtg attaacatac cgcccgctgc
     7021 cagtaaggtc atcgagacaa tcaggcggcg gcgttcaaac atatcaccga ggggaacaag
     7081 aaacagtaga cctgcggcat agcccaactg cgcggcggta acaataaagc ctgccgaact
     7141 ggcggaaagg gaaaagttac gcgcgatggt gtcgagcaat ggctgggcgt aatagttact
     7201 ggctaccgcc agaccggtgg cgatagacat cagcacgatc agcgccgggc taagctcatg
     7261 attaggttta gtcattattt ttgttcatca ggtgatgatt aagctggaaa tcaataatag
     7321 cggatgttga tgaatatggg atgaaagata gcttgttgaa atgtgcgatg tggtttgtag
     7381 gcatgatagg acgcgtcagc gtcgcatcag gcattgtgta cagagcggcg gatgaggcga
     7441 aaacgcctta tccgcccgaa taaaaattac ttctgcgctg ccagcgcctc attcacccag
     7501 ccatcgaact gctgctggtg ggctttgatc cagccatcaa cgtggccctg aatatcgcct
     7561 tctgaggctt tgccgtcatg cataatggcg ttctgggcgt taatatcggc cactggcaac
     7621 tgcataatgg caaacagttt ggctgccgcc ggatttttct cggtccaggc tttgttggca
     7681 acgatatgca tggtgctgac cgggaagccg taattcgcgc cattcggcag tttggtatcg
     7741 gcatttttat cgcccggcag tgcggagaac ggcacctgca accagaccac atctttccct
     7801 ggcttcagct cattactcac ccagtacggc gtccaggtgt aataaaacac cggtttgccc
     7861 tctttgtagc gactgatggt gtcggccatc atcgctgcgt agttcccctg attatgcgtc
     7921 acggtgttgg tcagttcata cgcggcaagc tggtggttga tcgcaccttc gcagccccag
     7981 ccagggttac aaccggttaa atccgcttta ccgtcgccgt tggtatcgaa cagtttggcg
     8041 atcttcggat ctttcagttg tgcgatgttg gtgattttgt attggtcggc ggttttctta
     8101 tcgatcaggt aaccctgtgc tgcgccgtta acaaataccc cttcacgata aaatttctta
     8161 tcgccaccgg cagcttcgta catgttgtca tggagtggcg tccagttcac ggcggtgaag
     8221 gttgcatcgc cggaagcaag cgaggtgtag ccaacgttgt aatccacttc gctgggtttg
     8281 ttgacggtat aacctaattt ctccagcgca cgactgacca gcagcgtctg gaaggtttct
     8341 tcagtgatgg tgctctgaac tggattaaca gtaatgcctt tgcccggcag atcggcagca
     8401 aaagtttgtg tagagataag cgtggcaaac gctgtcgcaa aaagtacgct atgtcgcatt
     8461 gttattcctt tttttggcag ggtgataatg cccggcgtca ccgggcaagt gcagagttac
     8521 ttaatgaatg ggcgggtcag cagaccaaca gggccagtgg tgtaccagcg acggttgccg
     8581 cgactgcgtg agtcgcgccc aacggcctgc gtcaggcgat cgaggataat ggcgaggatc
     8641 acaatcccga cgccgccaac ggtggcaagc cccatatcca gacgaccgat accgcgaagt
     8701 accatctgac ccagcccgcc gacggcaatc atcgaggcaa tgaccaccat agaaagggcc
     8761 agcatcagcg tctggttaac gcccgccata atggtcggca tcgccagcgg taactgaact
     8821 ttgaacagca tctggcgcgg gctggcaccg aatgagcgcg aggcttcaat cagatccgcc
     8881 ggaacctggt taatccccag aatggtcaga cggataatcg gcggcagcgc aaagatgatc
     8941 gtcaccacca cgcccggcac gttaccgata ccaaatagca tgacgattgg caccagataa
     9001 acaaacgctg gcgtggtctg catggcatca agcagtggac gaataatttt cgccgctcgc
     9061 ggacttctcg ccagccatat ccccaacggc aaaccgatga cgatacagaa cagcagggca
     9121 gttaacacca gcgccagagt caccattgcc tgcgaccagg caccgattgc gccgatggca
     9181 atcagcgaga ccagcgtcgc cacgcccatt ccgaccccgg aaatctgcca ggtgatgaga
     9241 gcgaaaacga taatcgccac tggcgctggc atacccagca gcaattgctg gaaaccgttg
     9301 aggatataat caaccggaac gcgcacgccc tggaagacgg gacggaaatg ggtaacaacc
     9361 cagtcgatcc cttcagtgac ccaactgtcg agcgggatca gcgttttatg gaacggatcg
     9421 agaatattaa aatgctcgac gtttggcgca ggcgtactgg tcagccagtc agcaccaccg
     9481 ccgtcagccg gtgcagtcgt cggtgtaccc caggcgtctg cggattgcgc ggcactgtcc
     9541 gccgctggcg tggtatccca cggattattt tgatcagcca ttatttaccc cctcacgatc
     9601 taaagcgcgc agcagcattc ctttcgaaat gatgccgaca tactgttggt cctcgtcgac
     9661 cacgggcacc gcacagggtg cctgtccgac atgagagagc aactcgctaa gaggcgtttg
     9721 tgcatcgact gctaacggcg catcaatcag cgccgcatca agaccttgct gctgcgttaa
     9781 cgcggtttta agcgaatcga tggagactgc gccgacaaac ttattaccgc gttcgataac
     9841 gtagccatat tcgcgatctt catcctgcaa taatttcagt gccgaacgtg ggccgaagcc
     9901 aggagtttta cgaattaagc cattcggtgt ccggcgggca atatctttcg cactgaacac
     9961 ctgactaata tcaacgccac ggaagaaggt acggacataa tcattcgccg gattattgag
    10021 aatttcatcc ggtgtgccga cctgtaccac ttcaccattt tgcataatgg caattcggtc
    10081 gccaatacgc atggcttcat caagatcgtg ggaaataaag acaatggtgc gctgatgttt
    10141 cgcctgtaat tttaccagct catcctgcat ctcggtgcga attaatggat cgagcgccga
    10201 gaaggcttcg tccattaata atatatccgg attaatcgct aacgcgcggg ctaatcccac
    10261 acgttgacgc atcccgccag agagttcatc cgggtagctg tgggcataat tttccagccc
    10321 gacctgacgc agtgcatcaa gggctttttc ccggcgttct tcggcattaa ttccggccaa
    10381 ttccataccg aacgcagtat tgtccagcac ggtcatatgc ggcattaagg caaaggactg
    10441 gaagaccatc gcaatctttt ttctgcgcac ctcacggagt tcggcgtcgg atattttggc
    10501 aatatccaca ccatcaatca gcacttgccc gcgggtgggt tcaatcaggc gattgagaag
    10561 gcgtaccatt gtggatttac ccgagccgga taatcccatg atgacaaata tctcgccttc
    10621 ttcaatggcc agactggcgt cttttacgcc aagcgatagc ccagtttttt ccagaatttg
    10681 ttcttttgaa agtccttgtt cgatatattt gaacgctcgc tgtggatgct caccaaatat
    10741 tttataaaga tttttaattt ttaatttaat ggccatgcaa tagaatgatt tctttatttg
    10801 tttatgtcga tatattaccc aatgggaata gtcacttttt ctaccctaac atactgagaa
    10861 tctgaggcaa cccctgaggg caaatgtggc atgaatattg atgcgagcaa atcgccggaa
    10921 ttttccgtga tataagggct gagagcaaat cgagaaaagt tgatattttt tgtcatagat
    10981 agcgaaaaaa cgcctgaata ttgtagtttc aggcgaataa tgcaacagag tatcagtgta
    11041 gatcactaca gaatatttgc gtgataaaaa gctatttggc gggaattatt tccctgctgc
    11101 gggtagtgat atttttgaaa ataacaccct cagaaattcc agtcttcatc ttctgtttca
    11161 accgctttcc ccatcacata agaggagcct gaaccggaaa agaaatcgtg attttcatcg
    11221 gcattcggcg aaagcgcggc gaggattgcc ggattcactt ccgccatttc tgcgggaaat
    11281 aacggttcgt agcccagatt catcaaagcc ttattggtgt tgtaacagag aaacgctttc
    11341 acatcgtcag cccacggggt ttcggcgtac agctcatcgg tgtattgcaa ctcgttgtcg
    11401 tagagttcca gcaacaaatc gaaggcgaaa ctcttcaact cttcacgttg tcccagagat
    11461 atcttttcca tgtttttctg atatttatag cctatgtagt aaccgtggac tgcttcatcg
    11521 cggataatca gacggatcag gtccgcggta ttggtcagct ttccgcggct ggaaaaatac
    11581 atcggcagcc agaaaccgga atagaacaaa aaagattcaa gaaacacact ggcgattttc
    11641 tttttcagcg gatcatcacc gcgataatgt tgctgaataa tctgagcttt tcgctgcaac
    11701 ggtgcgtttt cttcactcca ggcgtaggcg gcatcgacat ctttggtctg gcacagcgtc
    11761 gagaaaatcg aactgtaaga gcgggcatga accgcttcca taaagctgat attcgataat
    11821 accgcctctt catgaggcgt gagcgcatcg ggcatcagcg aaggcgcgcc gataacattt
    11881 tgcagcgtgt cgagcagcgt caggccagta aaaacgcgca tcgtcagttg ttgttctacg
    11941 acagttaatg tctgccaggc gggaatatcg ttcgacagcg gcaccttttc tggtagccag
    12001 aaattgctgg tcaggcgatt ccacacctcc agatctttat cgtcagatat cttgttccag
    12061 ttgatggcgc tgatacgtga gagtttcata gatattcctt aaagtgcaca ggagacgcag
    12121 ccttcaattt cagtgccttc cagcgccatc tgacgcaggc ggatgtaata gagcgttttg
    12181 atacctttgc gccaggcgta aatctgcgct ttgttgatat cgcgagtggt ggcggtatcg
    12241 gggaaaaaca gcgtcagcga cagcccctga tcgacatggc gagtcgcttc cgcgtaggtg
    12301 tcgatgatct tttctgcgcc aatttcgtaa gcgtcctgat acagcgccag attttcgtta
    12361 gtcataaacg gggcagggta gtaaacgcgt cctgttttgc cctctttgcg tatctctact
    12421 ttcgccacaa tcggatgaat actcgacgta gcatggttga tataagagat agacccggtt
    12481 ggcggcaccg cctggagatt ctggttgtat atgccgtagc gcatcacgtc gtcgcgcagt
    12541 tgcgcccaca tctcacgggt aggtaacgta atacagctgc gggcaaacag ttcgccaact
    12601 ttcgccgttt tcggctgcca gttaccttgc agatattggc tgaaatattc accactggca
    12661 tagcgtgact gtttgaaccc ggcgaaggtt tcaccgcgtt cgcgcgccag caacatcgag
    12721 gtacgcagtg cgtgccaggt gatggtatag aaatagagat tggtgaaatc cagggcttcc
    12781 ggcgaaccat aagcgatgcc ttccctcgcc agataaccat gtaaattcat ctgccccagt
    12841 ccgatggcgt gcgaggcggc atttccggct tcgatggacg gcacgctgcg gatatgactc
    12901 atatctgaca ccgccgttaa accgcgcacg gcagtctcta ccgtgcggga aaaatcgggg
    12961 gaatccatgg tgtgcgcaat attcaacgaa cctaaattgc aggaaatatc atggcctgtg
    13021 cgggcatagt cgagattctc gtcatactct gaggcgctgt taacctgcaa aatttctgag
    13081 cagagattac tcatatttat gcgcccggcg atagggttag cacggtttac tgtgtcttca
    13141 tacatgatgt agggatagcc ggactcaaac tggatttctg ccagtcgctg gaagaaatca
    13201 cgggcgttga ggtatttttt gcggatgcgt tcatcggcaa ccagttcgtc atagtgttcg
    13261 ctaatggcga tatcggcaaa cggcttgcca taaactcgct caacgtcata gggcgaaaac
    13321 agcgccatct gcgcattctc ttttgccaga tggaaagtga tatccgggat caccacgccc
    13381 agcgacagtg ttttaatgcg gattttttcg tcggcatttt cccgtttcgt gtcgagaaaa
    13441 cgcagaatat cgggatgatg agcatgtaaa tagactgcac ctgccccctg acgcgcgccg
    13501 agttggttgg cataggaaaa tgcgtcttcc aacattttca tcaccggaat tacgccagaa
    13561 gattgatttt caatacgttt aattggcgcg cccgcttccc gcagattcga cagcaaaaat
    13621 gctacgccgc cgccgcgttt cgacagctgc agtgcggaat ttaccgcccg accaatcgac
    13681 tccatattgt cttcaatacg cagcaaaaaa caggaaacca gttcgccgcg ctgctgctta
    13741 ccgcagttga ggaatgttgg cgtggctggc tgaaagcgtc ctgacagcat ttcatcggtc
    13801 agttgcaacg ccagcgtatc atcgccttgt gccagcgtca gcgccaccat cgtgactcga
    13861 tcggcaaaat cttccagata acgtttaccg tcgaatgtct tcaacgtata gctggtgtaa
    13921 aacttccatg ccccgaggaa tgtctggaaa cgaaaaccgc tggtgtgtgc gtgggcaaac
    13981 agcgtaatga caaagtcgcg agagtagcga ttaagaacgc tttcatcata gtaaccttcg
    14041 ttgaccagcc agttcaggcg ctgctgctgg ctactgaagg tcacactgtt cggacgcaca
    14101 tgcgtcgcaa taaaggcgtc aacggcctgg cggtctttat cgaactgaat gcgacctgcg
    14161 ctatcgtaaa gattaagcat cgcattcagc gcgtggtaat ccatcgtttc ctgcgtcagg
    14221 cattctgcgg ttgtcgttgc caaaattcgg ttactccttt acgaacgttt tcgatatcgc
    14281 tttgcgtacc catgagttca aaacggtaca gccacggcac gccgcatttc cgggcaatca
    14341 catctccggc gcggccatac gcctcaccaa agttgcgatt accagaagca ataacgccgc
    14401 gaagcaacgc ccggttgtgc tcgtcgttta aaaagcgaat tacctgtcgt ggcaccgcgc
    14461 cagccgtacc gccgccgccg taagagggca cgatcaggat gtaaggctcg tctacctgaa
    14521 tccgttcccg ctcattgagc gggatgcgca ccgcgggcag acctaaacgt tcgataaaac
    14581 gctgcgtgtt ttcggagctg ctggagaagt agacgagctg gctcatgcac tggccgcgtg
    14641 tggcgctgga tgcagacggt taatcatgtc cggacggaaa ccagaccagc taagatcgcc
    14701 agcaatcact accggcaact gacgaaagcc ctgagcacgc aacgcttccg ctgcttcagg
    14761 aacgcgatcg acattaatca tttcaaaatc aaagccccgg ttttccatcg cccgtttggt
    14821 ggcgtggcac tgaacgcaat cgttacgagt ataaatagtt atgcgcatga ttcgtatttc
    14881 cgtttaaaat gaagatacgg cgcgatgata cgcgtcgggt tgtctctctg ttgatacaga
    14941 gatactagat gtagttgaaa aaagattcaa ctacacaata tatagcaaat agtgatcgaa
    15001 attaccctgg atatgagcgt gacgggggag ggggattttt gtgattcacc aggcaaaaag
    15061 aaaccccgaa gacaggcttc ggggtcaaag acgcgtattt attatcattt ttgcactacg
    15121 atttgcgcat gcttaacagt gcgccgataa aaatacctac cgcagctgct gtacccacgc
    15181 tacaccaggg tctttcacga acaaaagaat ccgctcagcc aacggcatcg cgcgcggctt
    15241 gctggacgcg agtacgacca tgcattcgcg ccctggtttc tttcagcaac gcctgagctt
    15301 tactgcgtgc ggcttcagct tcccctttgg cgtcgctgcc ccaggacttc aatacagatt
    15361 cgaggctgtc agctaattga ttgacatcat tctggatatc ctgcacgcca tcatcaacgt
    15421 cgttgcggtt cggtcggtta aacatatgat cccccattag aattcgatgt aattttcagt
    15481 gtagaccata attttcttaa ctgaggttca tcgcggcttt atggactttt ctgaaagcgt
    15541 tgtgaatgcg caatcaggta aggtaggccc gcggttgaaa ataacgagga tcaaatatgt
    15601 atttacgacc agacgaggtg gcgcgcgtac ttgaaaaagt cggttttact gtcgatgtgg
    15661 taacccagaa agcgtatggt taccgccgtg gggaaaatta tgtctatgtt aatcgcgaag
    15721 cgcgaatggg acgtaccgct ctggtaattc atccaacatt aaaagaacgc agttcaacac
    15781 tggctgaacc cgcttccgat attaaaactt gcgatcatta ccagcaattt ccgctctatt
    15841 tagcaggcga gcgacacgag cattacggta tcccgcatgg ctttagttcg cgtgttgcgc
    15901 ttgaacgtta tttgaatggt ttatttggcg aagccagtta aagacgcgac tggcgatgcc
    15961 agtcgcgaaa agaagagtca ggcttttacc tgctggtaac ggctgacttt aaacagtcgg
    16021 cggcaataat cgaggaagta gccataaacc gcccccatca acatcgaaac aacgatgttt
    16081 gaactgaccg ccgccataat ctgatgccag tctgcgccca ccactaacaa gatcgccaca
    16141 tacaccggtg actggaacgt cacataagcc aggatatcag ccagattttt tatccagccc
    16201 gacgggctaa ctttgcgtgc cgcgcgcata aacagatcgc ggtacatacc gtatggccat
    16261 gcaattaaga tgttcaccgg aatcgctacc aatctggaat aaaaagactg ttcgaagctc
    16321 attccggaga ggaaaacttc aatacacatg ttcacgacag aacagtaaac aaccatcgcg
    16381 aacgtatctg caactgcatg acgcaagcgt gactgcggtg agaacatgct aatgctcctt
    16441 aataaaagcg taatgggatg ttaatggaga tgaaagcagt ggatctcatt gccatctgca
    16501 tttttgcata gcgtatatcg ctgtctatga ctaatcaatt agtgaaaaat ttttatttag
    16561 ttagtttttg tagataaaat attccgtaac ggctcgtttt ttgtacattg tggattttta
    16621 catgccaata tgatttattt tcaattaaat caattagata tgatcttgcc tttaatttca
    16681 ctaatgaaag tttggggggg aggtaacgct ataagcgtag agaattcggt gtttgtcatg
    16741 accgggtcat ttacagcatt tttgatgcca gaaaatgtac attaacagca gaaagcacca
    16801 gtgaggggtg ttttctttta ttctgttata ttgcattttt attcacttcg gttttgtcag
    16861 gcttgcggag ttagcgagca gagagcgcca ctctgttcac caataaccta aattactcat
    16921 caaaatggct aatatataat cttgaaatta ttctgagagt ttcagaaaat aacagctgaa
    16981 ataatctcgc gcaggactgt aaatagatta aattttgtgg aaatataata agtgatcgct
    17041 tacactacgc gacgaaatac ttttttgttt tggcgttaaa aggttttctt tattatgtcc
    17101 gtaatgttac aaagtttaaa taacattcgc accctccgtg cgatggctcg cgaattctcc
    17161 attgacgttc ttgaagaaat gctcgaaaaa ttcagggttg tcactaaaga aagacgtgaa
    17221 gaagaagaac agcagcagcg tgaactggct gagcgtcagg aaaaaattag cacctggctg
    17281 gagctgatga aagctgacgg aattaacccg gaagagttat tgggtaatag ctctgctgct
    17341 gcaccacgtg ctggtaaaaa acgccagccg cgtccggcga aatataaatt cactgatgtt
    17401 aacggtgaaa ctaaaacctg gaccggtcag ggccgtacac cgaagccaat tgctcaggcg
    17461 ctggcagaag gtaaatctct cgacgatttc ctgatctaat tactgaggcc ggatgtcgct
    17521 gctagggcgc gtccggcgtc gcttctcaag ctcgttggtt ccacggcatc ttatctaaca
    17581 accttgccat tccacaaaaa ccgctgattc ctgcaaacag taacccggca ccaacaaagc
    17641 cacttaataa gaagaaaccg ctatttacgg tataacccag tacaacgccg tttaatatta
    17701 aaccacccgc agcgatctgc acctggcgca ttaacggcaa gggttgagat ttatttaccg
    17761 ctactggcaa tccagctctt ttccagccat caatcccatc ttcgagtaaa aatatttctg
    17821 cgggggcggc aatcgctgct aatttatcgg cgttattact ggtgcgttta cctgcctggc
    17881 agtggaatat aatttgctcg cgacgtaatt taggtggaag acctgactgt tccagcacgg
    17941 ataatggagc cagatctgct tcaggaatat gttcacgaag atattcatca gcgtcacgaa
    18001 tatcgattaa ctttgcgccg cgtgcgatta attcttgtgc atcatgcggc gaaatggttg
    18061 tcaaagccat agtgactcct tacggacaat agacattttt cagggtggcg ataatggcat
    18121 ttaccgcctc atttttaatg gaatatagaa tgcgttgggc atcccgttgg ctgttgataa
    18181 gcccttcgtc ccgcatacga gcgagatgct gtgatgtcgc agaggcgctc agtccggtaa
    18241 tgcgcgtcag ctctcccgcg ctggtgccgg gggaaccgct aagcatgcac agaatcagca
    18301 accgtttagg gtggctcatt gctttcaata aggccgctgc ctgttcggta ctggcctgta
    18361 attgcgcgag ttcagtcata tttatttaag tattttctaa attaagtaaa ctctaaacta
    18421 aaaatgcaac atataccagc ctcagcagcg taaatgagag taaaagcgta agct
//
LOCUS       DAJTSI010000080        17781 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000080 DAJTSI010000000
VERSION     DAJTSI010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17781
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.080"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..246)
                     /gene="yefM"
                     /locus_tag="OO327_002445"
     CDS             complement(<1..246)
                     /gene="yefM"
                     /locus_tag="OO327_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCR6018956.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            529..570
                     /gene="hisL"
                     /locus_tag="OO327_002446"
     CDS             529..570
                     /gene="hisL"
                     /locus_tag="OO327_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR6018957.1"
                     /translation="MTRVQFKHHHHPD"
     misc_feature    546..669
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            716..1615
                     /gene="hisG"
                     /locus_tag="OO327_002447"
     CDS             716..1615
                     /gene="hisG"
                     /locus_tag="OO327_002447"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR6018958.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1621..2925
                     /gene="hisD"
                     /locus_tag="OO327_002448"
     CDS             1621..2925
                     /gene="hisD"
                     /locus_tag="OO327_002448"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR6018959.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2922..3992
                     /gene="hisC"
                     /locus_tag="OO327_002449"
     CDS             2922..3992
                     /gene="hisC"
                     /locus_tag="OO327_002449"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR6018960.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3992..5059
                     /gene="hisB"
                     /locus_tag="OO327_002450"
     CDS             3992..5059
                     /gene="hisB"
                     /locus_tag="OO327_002450"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR6018961.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5059..5649
                     /gene="hisH"
                     /locus_tag="OO327_002451"
     CDS             5059..5649
                     /gene="hisH"
                     /locus_tag="OO327_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR6018962.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5649..6386
                     /gene="hisA"
                     /locus_tag="OO327_002452"
     CDS             5649..6386
                     /gene="hisA"
                     /locus_tag="OO327_002452"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR6018963.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6368..7144
                     /gene="hisF"
                     /locus_tag="OO327_002453"
     CDS             6368..7144
                     /gene="hisF"
                     /locus_tag="OO327_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR6018964.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7138..7749
                     /locus_tag="OO327_002454"
     CDS             7138..7749
                     /locus_tag="OO327_002454"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR6018965.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7935..8912)
                     /gene="wzzB"
                     /locus_tag="OO327_002455"
     CDS             complement(7935..8912)
                     /gene="wzzB"
                     /locus_tag="OO327_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR6018966.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9058..10178)
                     /gene="ugd"
                     /locus_tag="OO327_002456"
                     /pseudo
     CDS             complement(9058..10178)
                     /gene="ugd"
                     /locus_tag="OO327_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10428..11834)
                     /gene="gndA"
                     /locus_tag="OO327_002457"
     CDS             complement(10428..11834)
                     /gene="gndA"
                     /locus_tag="OO327_002457"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR6018967.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11943..12302)
                     /locus_tag="OO327_002458"
     CDS             complement(11943..12302)
                     /locus_tag="OO327_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018968.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12299..12805)
                     /locus_tag="OO327_002459"
     CDS             complement(12299..12805)
                     /locus_tag="OO327_002459"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018969.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFILVCIIFFRGDRCILN
                     SNIFILNT"
     gene            complement(12951..13514)
                     /locus_tag="OO327_002460"
     CDS             complement(12951..13514)
                     /locus_tag="OO327_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6018970.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13721..14659)
                     /locus_tag="OO327_002461"
                     /pseudo
     CDS             complement(13721..14659)
                     /locus_tag="OO327_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14722..15096)
                     /locus_tag="OO327_002462"
     CDS             complement(14722..15096)
                     /locus_tag="OO327_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HCR6018971.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15100..16248)
                     /gene="wzy"
                     /locus_tag="OO327_002463"
     CDS             complement(15100..16248)
                     /gene="wzy"
                     /locus_tag="OO327_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HCR6018972.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16268..17173)
                     /gene="rfbG"
                     /locus_tag="OO327_002464"
     CDS             complement(16268..17173)
                     /gene="rfbG"
                     /locus_tag="OO327_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HCR6018973.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(17166..>17781)
                     /locus_tag="OO327_002465"
     CDS             complement(17166..>17781)
                     /locus_tag="OO327_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HCR6018974.1"
                     /translation="IRNEFIKKLSCYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGL
                     REKIHVTEGQTPFKSSVTISSGTMVSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGI
                     LVHIIPDIEMVHAIGDKSVKICGINIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIRE
                     VVFSLIHTTLIIATQKNKIEYMKKHILATLDGIRGITGGGRYNA"
BASE COUNT         5178 a         4115 c         3813 g         4675 t
ORIGIN      
        1 aataatgtcc ttttccgttc ctttgcctga tttcaggcta tcgattgagt ccatcaatct
       61 ccgggcgtta gcgggggaac gcagtagata agccgtctct tccagcgaat tgtattcttc
      121 gagtgacatc agaacacaag cctctccatt ctgacgagta atgaggatcg gggcatgatc
      181 ttcaacggct ttcatcattg ttgccgacaa attctgacgc gcttcgctgt agctaattgt
      241 acgcatgtaa atctcctatt ttgtacagtt cattgtacaa tgataggtgt taattaacta
      301 tttattaatt agtttgtaga tcaagacatt gccagcgaga cgaaaaatca gactttgctc
      361 tttttggtgc tgtcaagtta gaggacagtc ctcttagccc cctcctttcc ccgctcattc
      421 attaaacaaa tccatcgcca taaaatatat aaaaaagccc ttgctttcta acgtgaaagt
      481 ggtttaggtt aaaagacatc agttgaataa acattcacag agatttttat gacacgcgtt
      541 caatttaaac accaccatca tcctgactag tctttcaggc gatgtgtgct ggaagacatt
      601 cagatcttcc agtggtgcat gaacgcatga gaaagccccc ggaagatcac cttccggggg
      661 cttttttatt gcgcggttgt taacggttca gacaggttta aagaggaata agaaaatgac
      721 agacaacact cgtttacgca tagctatgca gaaatccggc cgtttaagtg atgactcacg
      781 cgaattactg gcgcgctgtg gcattaaaat caatcttcac acccagcgcc tgatcgcgat
      841 ggcagaaaac atgccgattg atattctgcg cgtgcgtgac gatgacattc cctgtctggt
      901 aatggacggc gtggtagacc ttgggattat cggcgaaaac gtgctggaag aagagctgct
      961 taaccgccgc gcccagggtg aagatccgcg ctactttacc ctgcgtcgtc tggatttcgg
     1021 cggctgccgc ctttcgctgg caacgccggt tgatgaagcc tgggacggtc cgctctcctt
     1081 aaacggtaaa cgtatcgcca cctcttatcc tcacctgctc aagcgttatc tcgaccagaa
     1141 aggtatctct tttaaatcct gcttactgaa cggttctgtc gaagttgcgc cgcgtgccgg
     1201 gctggcggat gcgatttgcg atctggtttc caccggtgcc acgctggaag ctaacggcct
     1261 gcgcgaagtc gaagttatct accgctcgaa agcctgcctg atccagcgcg atggcgaaat
     1321 ggaagaatct aaacagcaac tgatcgacaa gctactgacc cgtattcagg gtgtgatcca
     1381 ggcgcgcgaa tcaaaataca tcatgatgca cgcaccgacc gaacgcctgg atgaagtcat
     1441 cgccctgctg ccaggtgccg aacgcccaac cattctgccg ctggcgggtg atcaacagcg
     1501 cgtagcgatg cacatggtga gtagcgaaac cctgttctgg gaaacgatgg aaaaactgaa
     1561 agcactgggt gccagttcaa tcctggtcct gccgattgag aagatgatgg agtgatcgcc
     1621 atgagcttta acacaatcat tgactggaat agctgtactg cggagcaaca acgccagctg
     1681 ttaatgcgcc cggcgatctc cgcttctgaa agcattaccc gcaccgttaa cgatattctc
     1741 gataacgtga aaacgcgtgg cgatgaggcc ctgcgtgaat acagcgcgaa gtttgataaa
     1801 accacggtta ccgcgctgaa ggtgtctgct gatgagatcg ccgccgccag cgaacgcctg
     1861 agcgacgagc taaaacaggc gatggcggtg gcagtaaaga gtattgaaac cttccacact
     1921 gcgcaaaaac tgccgccggt agatgtagaa acgcagctag gcgtgcgttg ccagcaggtc
     1981 acgcgcccgg tggcttcagt gggtttgtat attccgggcg gctccgcccc gctcttctca
     2041 acggtattaa tgctggcaac tccggcgcgt attgcgggct gtaaaaaagt ggtgctgtgc
     2101 tcaccgccgc cgattgccga tgaaattctt tatgcggcgc agctgtgcga tgtgcaggac
     2161 gtgtttaacg tcggcggcgc acaggccatt gccgcgctgg cgtttggtac ggaatctgtg
     2221 ccgaaagtgg acaaaatctt cgggccgggt aacgcctttg tcaccgaagc aaaacgccag
     2281 gtaagccagc gtctggacgg tgcggcgatc gatatgcccg caggcccgtc ggaagtgctg
     2341 gtgattgctg acagcggcgc tacgccggat tttgtggctt ctgatttgct ttctcaggct
     2401 gaacacggcc ctgactcaca ggtaatttta ctgacccccg atgctgatat ggcgcgtcgc
     2461 gttgccgagg ccgtcgaacg ccaactggca gaactgccgc gtgccgaaac cacccgccag
     2521 gcactgaacg ccagccgcct gatcgtgact aaagatttag cgcagtgcgt ggagatctcc
     2581 aaccagtacg gcccggagca cctgatcatt cagacccgca acgcccgcga actggtcgat
     2641 ggcatcacca gcgccggttc ggtatttctt ggtgactggt caccggaatc ggcaggcgac
     2701 tacgcctccg gcaccaacca cgtgctgccg acttacggtt acaccgccac ctgttccagc
     2761 ctcgggctgg cagatttcca gaaacgcatg accgtacagg aactgtcgaa agaaggtttc
     2821 tccgcgctgg cttcgaccat tgaaacactg gccgccgccg agcgcctgac cgcccacaaa
     2881 aatgccgtta ctttgcgtgt taacgtcctt aaggagcaag catgagcacc gtgactatta
     2941 ctgatttagc gcgtgaaaac gtccgcaacc tgacaccgta tcagtcggcg cgtcgtctgg
     3001 gcggtaacgg cgacgtctgg ctgaacgcca acgaatatcc cacagccgtg gagtttaagc
     3061 ttactcagca aacgctcaac cgctacccgg aatgccagcc aaaagcggtg attgaaaatt
     3121 acgcgcaata tgcaggcgta aaaccggaac aggtgctggt cagccgtggc gcggacgaag
     3181 gtattgaact gctgatccgc gctttttgcg aaccgggtaa agacgccatc ctctactgcc
     3241 cgccaacgta cggcatgtac agcgtcagcg ctgaaaccat tggtgtcgaa tgccgcacag
     3301 tgccgacgct ggaaaactgg caactggact tacagggcat ttccgacaag ctggacggcg
     3361 taaaagtggt ctatgtttgc agccccaaca accccaccgg gcaactgatc aatccgcagg
     3421 attttcgcac cctgctggag ctaacccgcg gtaaggcgat tgtggttgcc gatgaagctt
     3481 atatcgagtt ttgcccgcag gcatcgttag ctggctggct ggcggaatat ccgcacctgg
     3541 cgattttgcg taccctttcg aaagccttcg cgttggcggg gcttcgttgc ggatttacgc
     3601 tggcaaacga agaagtcatc aacctgctga tgaaagtgat ccccccctac ccgctctcga
     3661 cgccggttgc cgacattgcc gcacaggcgt taagcccgca gggaatcgtc gccatgagcg
     3721 aacgggtggc gcaaattatt acagaacgcg aatacctgat tgccgcactg aaagagatcc
     3781 cctgcgtgga gcaggttttc gactctgaaa ccaactacat tctggcgcgc tttaaagcct
     3841 ccagtgcagt gtttaaatct ttgtgggatc agggcattat cttacgtgat cagaataaac
     3901 aaccctcttt aagcggctgc ctgcgaatta ccgtcggaac ccgtgaagaa agccagcgcg
     3961 tcattgacgc cttacgtgcg gagcaagttt gatgagtcag aagtatcttt ttatcgatcg
     4021 cgatggaacc ctgattagcg aaccgccgag tgattttcag gtggaccgtt ttgacaaact
     4081 cgcctttgaa ccgggcgtga tcccggaatt gctgaagctg caaaaagcgg gctacaagct
     4141 ggtgatgatc actaatcagg atggtctggg aacacaaagt ttcccgcaag cggattttga
     4201 tggcccgcac aacctgatga tgcagatctt cacctcgcaa ggcgtgcagt ttgatgaagt
     4261 gctgatttgt ccgcacctgc ccgccgatga gtgcgactgc cgtaagccga aagtaaaact
     4321 ggtggagggt tatctggctg agcaagcgat ggatcgtgcc aacagttatg taattggcga
     4381 tcgcgcgacc gacattcaac tggcggaaaa catgggcatt aatggtttac gctacgaccg
     4441 cgaaaccctg aactggccaa tgattggcga gcaactcact aaacgagacc gttacgccca
     4501 tgtagtgcgc aacaccaaag agacgcagat tgacgtccag gtgtggctgg atcgcgaagg
     4561 tggcagcaag attaacaccg gcgttggctt ctttgatcac atgctggatc agatcgccac
     4621 ccacggcggt ttccgcatgg aaatcaatgt caaaggcgac ctctatatcg acgatcacca
     4681 caccgtcgaa gataccggcc tggcgctggg cgaagcgtta aaaattgccc tcggcgacaa
     4741 acgcggtatt tgccgctttg gttttgtgct gccgatggac gaatgccttg cccgctgcgc
     4801 actggatatc tctggtcgcc cgcacctgga atataaagcc gagtttacct accagcgtgt
     4861 gggcgatctc agcactgaaa tgatcgaaca cttcttccgt tcgctctctt acaccatggg
     4921 cgtgacacta cacctgaaaa ccaaaggtaa aaacgatcat caccgtgtag aaagcctgtt
     4981 caaagccttt ggtcgtaccc tgcgccaggc catccgcgtc gaaggcgaca ccctgccctc
     5041 gtcgaaagga gtgctgtaat gaacgtggtg atccttgata ctggctgcgc caacctgaac
     5101 tctgtgaagt ctgccattgc gcgccacggt tatgaaccca aagtcagccg tgacccggac
     5161 attgtgttgc tggccgataa actgttttta cccggcgtcg gcaccgcaca agcagcgatg
     5221 gatcaggtgc gtgagcgcga gctgtttgat ctcatcaaag cctgtaccca accggtgctg
     5281 ggcatctgct tagggatgca actcctggga cgacgcagcg aagagagcaa tggcgtcgac
     5341 ttgctgggca tcatcgacga agacgtgccg aaaatgaccg actttggtct gccactgcca
     5401 catatgggct ggaaccgcgt ttatccgcag gcaggcaacc gcctgtttca ggggattgaa
     5461 gacggcgcgt acttttactt tgttcacagc tacgcaatgc cagtcaatcc gtggaccatc
     5521 gcccagtgta attacggcga accgttcacc gcggcggtac aaaaagataa cttctacggc
     5581 gtgcagttcc acccggagcg ttctggtgcc gctggcgcta agttgctgaa aaacttcctg
     5641 gagatgtgat gattattccg gcattagatt taatcgacgg caccgtggtg cgtctccatc
     5701 agggcgatta cggcaaacaa cgcgattacg gtaacgaccc gctgccgcgc ttacaggatt
     5761 acgctgcgca aggtgccgaa gtgttgcacc tggtggatct gaccggggca aaagatccgg
     5821 ctaagcgtca aatcccgctg attaaaaccc tggtcgcggg cgttaacgtc ccggtgcagg
     5881 ttggcggcgg tgttcgtagt gaagaagacg tggcggcgtt actggaagct ggcgttgcgc
     5941 gcgtggtggt cggttccacc gcagtgaaat caccggacgt agtgaaaggc tggtttgaac
     6001 gtttcggtgc cgatgcgctg gtgctggcac tggatgtccg tattgacgag caaggcaaca
     6061 agcaagtggc agtcagcggc tggcaagaga actcgggcgt ttcactggaa caactggtgg
     6121 aaacctatct gcccgtcggc ctgaaacatg tgttgtgtac cgatatctcg cgcgacggca
     6181 cgctggcggg atctaacgtc tctttatatg aagaagtgtg tgccagatat ccgcaagtgg
     6241 cgtttcagtc ctccggcggc attggcgaca ttaacgatgt tgctgcccta cgtggcactg
     6301 gcgtgcgcgg cgtaatagtt ggtcgtgcat tactggaagg taaattcacc gtgaaggagg
     6361 ccatcgcatg ctggcaaaac gcataatccc gtgtctcgac gttcgtgatg gtcaggtggt
     6421 gaaaggcgta cagtttcgca accatgaaat cattggcgat atcgtgccac tggcaaaacg
     6481 ctacgccgaa gaaggtgcag atgaactggt gttctacgat atcaccgctt ccagcgatgg
     6541 ccgtgtggta gataaaagct gggtatctcg cgtggcggaa gtgatcgaca ttccgttttg
     6601 tgtggcgggt gggattaagt ctctggaaga tgccgcgaaa attctttcct ttggcgcgga
     6661 taaaatttcc atcaactccc ctgcgctggc agacccaaca ttaattactc gcctggccga
     6721 tcgctttggc gtgcagtgta ttgtggttgg tattgatacc tggtacgacg ccgaaaccgg
     6781 taaatatcat gtaaatcaat ataccggcga tgaaagccgc acccgcgtca ctcaatggga
     6841 aacgctcgac tgggtagagg aggtgcaaaa acgcggcgcc ggagaaatcg tcctcaacat
     6901 gatgaatcag gacggcgtgt gtaacggtta cgacctcaag cagctgaaaa aagtgcgtga
     6961 agtttgccac gtcccgctga ttgcctccgg tggcgcgggc accatggaac acttcctgga
     7021 agccttccgc gatgccgacg ttgacggtgc gctggcagct tccgtattcc acaaacaaat
     7081 aatcaatatt ggcgaattaa aagcgtacct ggcaacacag ggcgtggaga tcaggatatg
     7141 ttaacagaac aacaacgtcg cgaactggac tgggaaaaaa ccgacggtct gatgccggtg
     7201 attgtgcaac acgcggtatc tggcgaagtg ttaatgctgg gctacatgaa cccggaagcc
     7261 ttagacaaaa ccatcgaaag cggtaaagtc accttcttct cgcgcactaa acagcgactg
     7321 tggatcaaag gcgaaacctc gggcaatttt ctcaatgtgg taagtattgc cccggactgc
     7381 gacaacgaca cattactggt gctggcgaat cccatcgggc caacctgcca caaaggcacc
     7441 agtagctgct tcggcaacac cgctcaccag tggctgttcc tgtatcaact ggaacaactg
     7501 ctcgccgaac gtaaatatgc cgacccggaa acctcctaca ccgccaaact gtatgccagc
     7561 ggcactaaac gcattgcgca gaaagtgggc gaagaaggcg tggaaaccgc actggcagca
     7621 acggtacatg atcgctttga gctgaccaac gaggcatctg acttgatgta tcacctgctg
     7681 gtgctgttgc aggatcagga tctggattta acgacagtaa ttgagaacct gcataaacgg
     7741 catcagtgag ttacgggctg agcaggtgtg atgttgttgt cacacccgga gccattgccc
     7801 gatgcgcaaa cttatcgtgc ctacacatta tctcaacaca cgtaggccgg ataagatgca
     7861 ccagcatcgc atccggcaaa aaaaacgggc atggtgtcac caccctgccc gtttctctta
     7921 aatgcacaat aatattactt cgcgttgtaa ttacgcagag cgttacgccc caacacaatc
     7981 cccgcgccaa ccatgccgcc cagaagcact gccagaatca aggtgattgc ctttttcgga
     8041 ctatcgcgac gaataggtaa cgtcggtttc atcacatagc ggtaagcatg aatatcaaga
     8101 tcatcaaact ttaatttttc aatatccaac aggttttgac gtgtctgata gtagtttggt
     8161 gagaacacca acggacgagt cgcttcatgc ttaatcatcg actccagcgc ttcgctccct
     8221 agaaggaaca acgtatcttg cgtcacatct tcagtctgct gaacctgtgg ctttgtcacc
     8281 tgcgcctgat tcgcatactg caacgcttcc tgaatctgac ggatacgcag atctttctgc
     8341 tcctgcgcga ccacttcctg ggttcttaaa gagtcctgca agtttttccg tcccagagca
     8401 atgttgtcct tgagatcctt ttctagctct tgattcactt tatcatcaac ttgctgaatg
     8461 tattgggcca acttcatttg tgcgccctct gcagtttgcc caacataaga aacagtcaat
     8521 ggtaattgct ggttcttaac agaaggttcg atggtaagtt tttctggctc ttcctgatta
     8581 tccagcgttt ctgctaatgc agagaaggca gaactgaagc gaccaattaa cgtctcctgc
     8641 aaatccgata ctttcggtgc agcctgacca tagataacat tcatggcatt gttatagcca
     8701 gcaatttgcc ccacgtcggg ctgagtgata attgctgttg acgtccattt ctccttcgct
     8761 actgccaaat aaccaatagc cagggcaata gccacaatga cggaaatgat aattgtcatc
     8821 ttgccacgcc acaactgcac tagtaaatca atcaaatcaa tctgttccgg gtcatggttt
     8881 tgcccagaaa cattattatt ttctactctc atcattaccc taactgacgg aaatttccaa
     8941 gtccgcatag tttagcgata atccccgtga atgctatagg ataagtgata aaaataatga
     9001 attattgcgg aggtaacaaa agacaatcag ggcgtaaata gccctgataa caagatgtta
     9061 gtcgctgcca aagagatcgc gggtgtagac cttatctgcc acatccttaa gctcttctgc
     9121 catacggttg gagataatga cgtcggcttg ttgtttgaag gtggcgagat cacgttccag
     9181 gcgagagttg aagaatgagt cttctttcat caccggctcg tagatgatca cttcaacgcc
     9241 tttcgccttg atacgcttca taatcccctg aatagaagac gcacggaagt tatctgaccc
     9301 gctcttcata atcagacgat aaatacccac cacttgcggt ttgcatcgac aattgccgag
     9361 atcaggttat tcggcacaga ctggtagttc gccagtaact gcttggtatc tttcggcaga
     9421 caataaccac cataaccaaa cgacggattg ttgtaatggt tgccaatacg cggatcgaga
     9481 caaacgcctt cgataatctg gcgagtattc agacctaaac tttctgcata actatccagt
     9541 tcattaaagt atgctacgcg catcgccaga taggtattag cgaaaagttt aatcgcttct
     9601 gcttcagtgg agtcggtaaa cagggtcggg atattttgct taatcgcgcc ttcctgtaac
     9661 aacgcagcaa aacgttctgc gcgttctgaa cgctcaccga tgacaatacg tgacggatgg
     9721 agattatcgt aaagggcttt accttcgcgg aggaattccg gagagaaaat aatattttca
     9781 gtacgatatt tcttatgcat cgctgcggta aaaccaacgg gaaccgttga tttgatgacc
     9841 ataaccgcat aaggatttat ctcaactacg tctttaatta ctgattctac actggatgta
     9901 ttgaaataat tagttttagg atcatagtcg gttggagtgg cgatgatgac ataatcagca
     9961 tcccggtagg cttcattttt atctaatgtg gcattaaagt gtattttatc tgattgcaaa
    10021 aactgctgaa tttccttatc aacaatagga gatatccgat cattcagcat agcaacgcgt
    10081 gacggtaaaa tatctaatgc cacaacctca tgattttgtg cgattagaag cccgtttgac
    10141 aagcctacat agccagtacc ggaaatggtg attttcattt catgctctca gaattaactt
    10201 aactgtgaat catgatgttt ttagcatcgt gataagatgc taaaagtttt aacgctacgt
    10261 tgtcagatgg tcaacgcaac gaaaaacaat tcagattttc tttataagaa tagctgatgt
    10321 ttattaagtt aatattaagc agtgaaattt agtctataag atatttggca aaaaaaagcc
    10381 cggtgcaata tacgccgggc ctcaatttta ttgttggtta aatcagatta atccagccat
    10441 tcagtatgga acacaccttc tttatcaatg cgcttataag tatgcgcccc gaaatagtca
    10501 cgctgtgcct ggatcaggtt cgcaggcaga accgcggcac ggtagctatc gtaataggca
    10561 accgcagcgg cgaaggtcgg aaccgggata ccgttctgta ctgcataagc gacgacatcg
    10621 cgcagcgcct gctggtagtc atcggcaatt tgcttgaagt acggggccag cagcaggtta
    10681 gcgatctgcg gattttcggc ataagcatcg gtgattttct gcaggaactg cgcacggatg
    10741 atgcagccag cacggaaaat cttcgcgatt tcgccgtagt tcagatccca attgtattct
    10801 tcagacgcag cacgcagctg agagaagccc tgagcgtaag aaacgatttt gcccagatac
    10861 agcgcactgc gaactttttc gatgaactca gccttgtcgc ctgctgactg agcttgcgga
    10921 ccagagagaa ctttagatgc ggcaacgcgc tgatctttca gagaagagat ataacgtgca
    10981 aacacagact cggtaatcag cgacagcggt tcgccgagat ccagcgcgct ctggctggtc
    11041 catttaccgg tgcctttgtt tgctgcttca tccagaatca catcaaccag gtagttaccg
    11101 tcttcatctt ttttggtgaa gatatcttta gtgatgtcga tcaggtagct gctaagttca
    11161 ccgttattcc actcggtgaa agtctgggcc agttcttcat tagatagatt caggccacct
    11221 ttgagcagag aatacgcttc agcaatgagc tgcatatctc cgtattcaat accattgtga
    11281 accatcttca cataatggcc tgcgccgtcg gcaccaatat aagtaacgca tggctcgccg
    11341 tcttctgcca cggcagcgat tttagtcagg ataggcgcaa ccagctcata agcctctttc
    11401 tgcccgccag gcataataga aggacctttc agcgcgccct cttcaccgcc ggaaacaccg
    11461 gtaccgatga agttaaagcc ctctgctgaa agctcacgat tacgacgaat ggtgtcctgg
    11521 aagaaggtat taccaccatc aatgatgatg tcacctttat cgagatatgg tttcagggaa
    11581 tcaatcgcag catccgtgcc tgcacctgct ttcaccatta acaggatgcg acgaggcgtt
    11641 tccagagatt caacaaactc tttcaccgta tagtaaggag ccagtttctt gcctggattt
    11701 tcagcaatca cttcttccgt cttttcgcgg gagcggttga aaatagagac ggtataacca
    11761 cgactttcga tgttgagcgc aaggttgcgc cccatcactg ccataccgac tacgccgatc
    11821 tgttgctttg acattgttta ctcctgtctt ctatattatt aaccgtctat taaccgttat
    11881 attattaacc gtcgtgattt tcatcttcta atcaaggaat acatatggtt acagcatgct
    11941 tcttacttta tagcgataag tatgccattg ggtgtatcca taagaacttt gaattttgga
    12001 tacttttctg gattaaaatt agcatagctt cgtttagata taacaataaa cgaactatta
    12061 aaagatttca tcttctctat taaatcatcc tgtgggatta cctcacactc gggattacta
    12121 atagtccaat cactatatct tgctaatcca aagacgtcac caattgccat ccctttatag
    12181 aagtaaatac catcctccaa gcctaattgg ataagattat ctccatataa ctcactatat
    12241 tcacttgctt tcttcatcag ttcatagcta tagcgacttt ctaagcgtga attaaaattc
    12301 aagtattcag aataaaaata tttgaattca aaatacaacg atcgccccga aaaaaaataa
    12361 tacacacaag gataaaatat cgtcccaact tgtaatttgt aatgttgata tcttttatta
    12421 aacttgcaat tgagatcgca acctgcaata ctgacaatgg gtaaatgggt gataaatacc
    12481 tgtcaccttg cgtaacagaa aaccaaaata agacatatgt taaaacaatc gagaaaaata
    12541 cccaaagtat tttattattc ctaagtgtaa agacagacag tatagcataa aaaacaacat
    12601 cactgccaac tttattaatt gcttgttgca aatctaatgt ggcaggactt ataccatgtc
    12661 gagcttgttc tgctgtttga acttgtagat caatttcatt ccataggtag tgaccaaata
    12721 tatttcctcc agcaggacta aaaggatcac cagagatcca aaaactacgg acataccaat
    12781 aaatgccacc aagaattgtt gccaagctaa aaataagaaa tgtctgtatg tttctctttt
    12841 tcgctatcaa tataacaaat atgatggctc catatatggc accaaatagt tttacgccac
    12901 aagcaattcc aataaaaaaa ccacataaaa aaattaattc attttgtgtt ttattgtttt
    12961 ctgagcgaat atatataaga atactggctg cagcaaaaca aagatagcgt taaatcaata
    13021 tatgcataac caagatgatc tttaagtgga ttgatagtaa atagtaggat tgtagcaata
    13081 atgcctggat aataagtttt cgtaatttcc catgaacagc caacaagtcc acaggctatt
    13141 acaaaaaaac agatatttgc taaaaactga gctaacctta catctcctaa ctgtaaacct
    13201 aaagccatta aaagatctgc attttgcggg aacagaggaa aacgcaaata ttgttcaaca
    13261 acaattgcat ggtgctcaac taatgaacga gccaaaggta agtgatacat agtatcatcc
    13321 caatcaccgg gagctcgcag ggacgataac aatgtaacca caaaaatcaa aacaagaaat
    13381 aaaataacaa acagcgtact gaactcaata ttatttattg ccagccatct aaattgctga
    13441 cgtttaataa tgcttaaaac ccaacacacc gtaaaaatta aaaagatagc aatcttatta
    13501 aattgcccga ccatgcccag aaagtaaatt atcaaaatat ttaaagacat ccctatgcat
    13561 aggcaataaa aaataaagaa aatttgttaa gtcctgccac cccctcctta aaaaaagatg
    13621 acaatacaat attaccaatg acaaaaaatg tcattccaaa agcaaaacaa tacacaacat
    13681 acaaatacaa cggcaattca atcatagtaa aatactctat ttacattatc agtttatagt
    13741 ttttttcaga aagataaaat aatgccttca tttcacgtct agttgttgta tttgaatgca
    13801 aaataatgcc acatacaaat gccattattg acgataacat gatggaggca gttaaaaagg
    13861 cagtcggaaa tcttctcacc aaacctgtat caatataatc aacaatgatg ggcaatccta
    13921 aaattataga caaagcagca agtaaaacgc cgataatact aaaaaagtat agtggccgtt
    13981 caagcgagta aagtttaatt attgttttca gaatcttgaa cccatcactc caagttgata
    14041 gtttgctcac actcccttca gatcgctcgc catattttgt attaacctca ccatacttca
    14101 tacgtaattc taaagcatga attgtaagct cagtttcgat ttcaaatccc cttgatagac
    14161 atggaaaaga tttaacatag cgtctggaaa atacacgata ccccgacaac atatcagtaa
    14221 aattcccttt aaagatagtc tgaacagtac cagtcaacaa tttatttcct cagcgatgcc
    14281 cttttctgta tgtattttga tctccattct cttgacgaca tcctacaacc atatccaatt
    14341 gattttttat gagatggtta atcatttctg gagcagaact tgcatcataa gtgtcatctc
    14401 catcaaccat tagataaata tccgcatcta catctgagaa catacgtctt acgacattcc
    14461 ccttgccttt cagaggaaca gagtgaactg ttgctccagc atcctctgcc acttgtgctg
    14521 tactgtcagt cgaattatta tcaaatacat atatagaagc agttggaata ttcgttttga
    14581 aatcatttat gacttttgct attgccttct gctcattata acaaggaata attacagcga
    14641 tattaaaaga gtgatacata agcccctcat ataaactatt tagctaatgt gcaagtaatt
    14701 gtgtttttaa caaatcgttt actaacgaaa aatgaaatat tttgatgcgc aatatcctaa
    14761 aaccaaacta aaaagagaaa aggtgaacaa tgtaaatatt ggagataaag ctaagagatc
    14821 aaataaaacg ccgaataaat aactcatcgc ccccataaaa aatataaata taaaatatct
    14881 agccttggtt gcagatactt taaatgagca tcttgcattt gcaaaaaaag aaaaggttgc
    14941 tgcacatata aaaccaacaa tgttacttcg gccttgacca aaagctaaat tattataaca
    15001 cgcataaaat accccccaat gtaaagcagt attgacaatt ccaattaaaa aataactaaa
    15061 aaaagaactg gtcaataact tccctatttt taacatcctt tattttgctc cagaagtgag
    15121 gttattacta atttggatat tttctataga aaatacccat aaaactacaa cgcctataat
    15181 aaaaattccc atgggtgtaa aaagaaatgg attagttata gaaacggtaa tactgaaaaa
    15241 aagtattaac gacatcatta atgatgtttt tgtactattt aaaaaatagc ctttcaaaaa
    15301 gataagcaac ataggaacta atgatataaa tacaccaata acgcctgttt tctgaagtat
    15361 ctcaagaggt actatttcaa tccttaatcg atctaatata aatgaaccaa atcctcttcc
    15421 aaacaagaac gtcgctaaat caacattttt ataataaaaa tataaatcat taaatctaac
    15481 agagtcagaa tcttcactgc gtgatccgac catgtaaagc ataattataa atagaacgct
    15541 gcccaatatt attataatag tatttaaata tagcttatgt aatttaaaac tggctaataa
    15601 cagtgataat gttgtaaaaa gtaatacacc tcttgattct gtcaataata tcgcaagcac
    15661 tattatgaga cattttaaat attttttgtc gacaaaactg ataatcgcac cgacaccaaa
    15721 aaagtagaag gatttggaaa aaaaagcccc atcgggacga aaaaagaact cgctagtcaa
    15781 tgataagtgt tcataaatta aattaaaatt taacaaaccg aaattcaaca aaattatgta
    15841 tgaaatatat aacagtgatc catataatgc aaatgcatta actattttaa taaaaatctc
    15901 acatgaaaat acttttaaag tatatcttag tgtaaataga tatataagtg acagcataaa
    15961 aaggtatggt tgcacatcaa acagtatatc atttatttta ttaccagcta ataagccgac
    16021 aaagaagcca taaaaaggaa ataaaagcaa ccatataaaa agactgacac atttttgggt
    16081 gattgaaaaa ttgaatgtaa ctaaattaaa aattaaaagt ataaaactaa atacaaataa
    16141 taaatatcgc aaagggaata ttcccaatgg ctccagtaat cttccaccac caccaataat
    16201 cagttcaata cataaaaatg ttataaaaat tttatttata ttattcatat tcgtaaggtg
    16261 atgtttttta aaatatgttt aaaatcaaca ttaataaaat ataaatttta tcagcctttt
    16321 tccgccttaa cttccaaaaa cttaaagcca agatcaaatt gcctatataa cctttaccca
    16381 attgattatt cacaaagcta tcatcagcta aggcattagc tattttagtt acttcttttc
    16441 gataccactt gtttctcacc aaaccaagcc gtttgtattt tgctttaaaa ctaatgtttg
    16501 ctcccacttg gttgttttca tgctgtctgt acagcattgt tggttttcta tcaatatacc
    16561 acgaataatt tctcgtccgc gcaaatgaat ataaaaacca atcatgcaga cagatatctt
    16621 gtgcagcatt tttattattt atgatgaact tcttaaattc aataagcgtt tcttttttta
    16681 taacatatgt acaccccgga cctgctgctt caaaaaaata atcgaattga gtttgcgact
    16741 gagccttatc aactaaattt tttcgccctg atggataata tgcaattaca ttgctggaat
    16801 aacaattcgc attattaaat accaagcagt caattgcatt ttttatttta tctttatacc
    16861 aaatatcgtc ctgatcacta aaacagatat aatcatagtt ttcaagatcg acatctttaa
    16921 ttaatctata aaaattttta cctgcccccc caaacttatt gacgcttgga agtatattta
    16981 taattgaggg atatgataat tgaaattctt cacaaatatt taatgtatta tcagttgata
    17041 aatcatctga tataaaaatt gagatgtcta catctttttg ttcaataata ctttttaatt
    17101 gttcctcaat ccagcattcg ccattatacg tagccataag tacagctact cttttttgat
    17161 cagaactatg cattataacg accgccccca gtaattcctc ttattccatc cagcgtagcc
    17221 aaaatatgtt ttttcatata ttcaatttta tttttttgag ttgcgataat taaagtcgta
    17281 tgaattaaag aaaaaacaac ttccctaata gagagtaaaa gaggcacatg attttttcta
    17341 agcaaaagaa atgcatttct tactcgataa taacgacgta ctggcgagtg aattggtatg
    17401 ttaatcccac agatttttac tgacttatcc ccaatagcat gaaccatttc tatatcagga
    17461 atgatatgaa ctaatatgcc atagtttaag catctaagac accattctgt atcgacataa
    17521 tcaataaaaa gttcctcatc catcattcca acaatctcaa aaacttcttt tgaaaccatg
    17581 gttccggatg agattgttac tgaactttta aacggtgtct gtccttcagt gacatgaatt
    17641 ttctctcgaa gaccattttt tttgatatta caaataggat aataatgtga cttatctcta
    17701 tcaataaaaa ctgggccagc tatttttgca ttttcattat ccatatagca ggaaagtttt
    17761 ttgataaact cattgcgaat a
//
LOCUS       DAJTSI010000081        17639 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000081 DAJTSI010000000
VERSION     DAJTSI010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17639
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.081"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            98..554
                     /locus_tag="OO327_002466"
                     /pseudo
     CDS             98..554
                     /locus_tag="OO327_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            614..721
                     /locus_tag="OO327_002467"
     CDS             614..721
                     /locus_tag="OO327_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR6018975.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(702..1433)
                     /gene="yehW"
                     /locus_tag="OO327_002468"
     CDS             complement(702..1433)
                     /gene="yehW"
                     /locus_tag="OO327_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR6018976.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(1438..2364)
                     /gene="yehX"
                     /locus_tag="OO327_002469"
     CDS             complement(1438..2364)
                     /gene="yehX"
                     /locus_tag="OO327_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR6018977.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(2357..3514)
                     /gene="yehY"
                     /locus_tag="OO327_002470"
     CDS             complement(2357..3514)
                     /gene="yehY"
                     /locus_tag="OO327_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCR6018978.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(3521..4438)
                     /gene="osmF"
                     /locus_tag="OO327_002471"
     CDS             complement(3521..4438)
                     /gene="osmF"
                     /locus_tag="OO327_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR6018979.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(4690..6987)
                     /gene="bglX"
                     /locus_tag="OO327_002472"
     CDS             complement(4690..6987)
                     /gene="bglX"
                     /locus_tag="OO327_002472"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR6018980.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            7183..8898
                     /gene="dld"
                     /locus_tag="OO327_002473"
     CDS             7183..8898
                     /gene="dld"
                     /locus_tag="OO327_002473"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6018981.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(8936..9868)
                     /gene="pbpG"
                     /locus_tag="OO327_002474"
     CDS             complement(8936..9868)
                     /gene="pbpG"
                     /locus_tag="OO327_002474"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR6018982.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(10042..10629)
                     /locus_tag="OO327_002475"
     CDS             complement(10042..10629)
                     /locus_tag="OO327_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR6018983.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            10799..11377
                     /locus_tag="OO327_002476"
     CDS             10799..11377
                     /locus_tag="OO327_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6018984.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(11494..12255)
                     /locus_tag="OO327_002477"
                     /pseudo
     CDS             complement(11494..12255)
                     /locus_tag="OO327_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(12308..13744)
                     /gene="mdtQ"
                     /locus_tag="OO327_002478"
                     /pseudo
     CDS             complement(12308..13744)
                     /gene="mdtQ"
                     /locus_tag="OO327_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            13968..14051
                     /gene="yohP"
                     /locus_tag="OO327_002479"
     CDS             13968..14051
                     /gene="yohP"
                     /locus_tag="OO327_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCR6018985.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(14422..15369)
                     /gene="dusC"
                     /locus_tag="OO327_002480"
     CDS             complement(14422..15369)
                     /gene="dusC"
                     /locus_tag="OO327_002480"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR6018986.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            15608..16006
                     /locus_tag="OO327_002481"
     CDS             15608..16006
                     /locus_tag="OO327_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR6018987.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            16003..16698
                     /locus_tag="OO327_002482"
     CDS             16003..16698
                     /locus_tag="OO327_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR6018988.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            16828..>17639
                     /gene="cdd"
                     /locus_tag="OO327_002483"
     CDS             16828..>17639
                     /gene="cdd"
                     /locus_tag="OO327_002483"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR6018989.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPL"
BASE COUNT         4135 a         4890 c         4461 g         4153 t
ORIGIN      
        1 cagaagcaga aaaagcttat tatgcttcca tcggaaacga tgatctggca gcctgagttc
       61 acagataaaa cactctccag gaaacccggg gcggttcagc ggcaatctac atagcctgcg
      121 aacgtggatc aaagagcgcg gtcaggatta ccccgcccag acactcacca cacatctgtt
      181 tattcctctg cgccgacggc ttcagtgcca acaaccgact ctccaggcgc tgctggcgat
      241 cctcgacggc gtactgatca actacatcgc catttgtctg gcttcggcac gtaaaaaaca
      301 gggtaaagat gcgctggtgg ttggctggaa tattcaggat accacccgtc tgtggctgga
      361 gggctggatt gccagtcaac aaggatggcg cattgatgtc ctcgctcgcc cactcgctca
      421 atcaactacg ccctgaacta ttcgaaggcc gtacattgct ggtgtggtgc ggtgaaaatc
      481 gaacctccgc ccaacagcag caactcacca gttggcaaga acaaggccat gatattttcc
      541 cactcggtat ttaatgattc gttaacaaat gcgctttact gtacaatcct ttcgttaaca
      601 taaggagtgc attatgcgca tagctaaaat tggggtcatc gccctgttcc tgtttatggc
      661 gttaggcgga attggtggcg tcatgctcgc aggttatacc tttattttgc gtgctggcta
      721 agcgcctgca ccagcctttc aaacaggcgg tctgcgatga tcgccgccag tgccaccagt
      781 aacgccccct ggatcacata cgcggtatta aatccgctaa gcccgatgat gatgggcgta
      841 cccagcgtac tggcccctac cgttgaggcg atcgtcgccg taccaatgtt gataatcacc
      901 gaagttcgca cgcccgccag aatcaccgga gccgccagcg gtagctcgac cttacgcagt
      961 cgctgacctc gactcattcc catacctttc gcaacttctg tcacgctggg atcaatcgct
     1021 cccagcccgg caagtgtcac ctgcaggatg ggcagcacac cgtaaaggat caaggcgata
     1081 atcgctggtt gcagaccaaa gccgatcacc ggaacggcaa tcgccagcac tgcgacaggc
     1141 ggaaaagtct gtccaacggc ggcaatagtt tccaccagtg ggcgaaattc cgcgccccac
     1201 gggcgagtaa cagcaattcc ggcacctgtg ccaatgatca ccgcaaacaa actcgaaatt
     1261 cccaccagcc agaaatgagc cagtgccaga gctgcaaaac tttcttgctg ataaacgggt
     1321 cgtggcagtt gtgggaacaa ggcagcaaac agcggctggc tgtaaggcag ccagaaaatc
     1381 agcgccacaa aaagagcaat gagccagaac agcggatcgc gcaacatctt catacgctta
     1441 cgcctccacc agcagatcct gaaaatgcag cgtgccgcaa ggctggccct gcgtgttcac
     1501 caccggcagc acctcgcatc cccgcgcgac aaacagggag agcgcatcgc gtagcgtcat
     1561 ctcttctgcc agtgcctcac cttctgcccg ttcttcgcga cgcacgtaat ccgccacgct
     1621 acgtaacgaa agcaggtgca cccccagttc actacgtcca aaaaactggc ggacaaaatc
     1681 attcgccgga cgagtcagca tcgtcagcgg attcccctgc tgcaccactt caccgtgatc
     1741 catcaatacc agatgttctg ccagccgtag cgcctcatca atatcatgag tgaccagcac
     1801 aatagtgcgc cccagcaaac ggtgaatgcg cgtcatctct tgttgcaacg cgccgcgcgt
     1861 taccgggtcc agtgcgccaa aaggttcatc catcagtaag acttgcggat cggcagccag
     1921 tgcacgcgcc actcccacac gttgctgctg accaccggaa agctgatgcg gataacgctc
     1981 acgcaaattt gactccagcc ccagtagcgc cattaattcg tcgatacgat cgtcaatccg
     2041 cgcccgcgac catttttgta attgcggcac ggtagcgatg ttttgcgcca cgctccagtg
     2101 ggggaacagg ccaatagatt gaatggcata gcccatccgg cggcgcaact ccagtactgg
     2161 cagcgagcga atttcttctc cggcaaagcg gatcactccg ctgtcatgct ccaccaggcg
     2221 gttaatcatt ttcagggtgg tggatttgcc ggagccagat gtgccaatca gcaccgaaaa
     2281 actcccttcc tgaaaattga gattgagatc gttaacggct ttttgtgcgc cgaacagttt
     2341 gctgacatgg ctaaattcaa tcattacgtt tcaccttcag cagtgcgata agcaaatcga
     2401 acagcgcgtc ggtaagcacc gccagaacaa ttaccgggat cacccccagc aacactaaat
     2461 caatggcgct gcttagcagc ccctggaaaa ccagcgcacc aaaaccgcct gcgccgatta
     2521 acgccgcaat caccgccata cctacagttt gcaccatcac cacccgcagg ctgcgcagaa
     2581 ataccggtaa cgccagcggt aactgaacat gcaggaaacg ctgcgccccg ctcatcccca
     2641 tcgctctggc gctctccagc acatcgcgcg ggatctggtt caaaccgact accacgcctc
     2701 gcaccagcgg cagcaaggca tagagcacca gcgcaatcag tgcgggtgtc attccggttc
     2761 ctgctatgcc gagcttcccc agccacggaa aggccgtaac cagcgcggca agcggcgcaa
     2821 tcaacaggcc aaagagcgcc accgaaggca cggtctgaat gacattgagc agagaaaaaa
     2881 ttgccccctg ccgcgcagtg gaaaagtagc accagatgcc caacggcaca ccaatcacta
     2941 acgcaggcag caccgcacca aacagcaacg tcagatgttg tgccagcgcg tcgtcaaaca
     3001 catcctgacg gttggcgtat tctttcatta gtgagagatc gttaagcgtg ccggagtaca
     3061 gcaaccacag cggaataatg gcaatctgca tatgcaacaa ccagcgccac agcggatgcg
     3121 tggagattcg gcggatggca tcgctacagg ccagcaatgc cagcgccgca gccagccaga
     3181 aacctctgcc gaggctggta cgcgccagcg cactgccatt ttgcgccagt tgggtcgccg
     3241 cctttccagc tccccacacc aacaatacga agacgaattg cgccagaatg agtgcaaaaa
     3301 tgctgccttt tttagcgggg acaaaacagg ctgtcagcca ggcgcaacca acgcccacca
     3361 gcatccagat cgtttgcggc cacagttgcc agagatgaag cccttcaccc gagactaaac
     3421 gattaggcgc gtaactgata aacggcagcg ccgctgcgat tgccgtcagc aacaacagca
     3481 gcgccagaac aggattaata cgtagataag tcacgggaaa ttacttcgcc cacccttttt
     3541 gtttcaggta gtcggcagcc acttttttgg catccagtcc ttccactgca atgctggcat
     3601 tcagttgctg caatgttttt gcatcgaggc tggcgaagac tggctgtagc cactgtgcca
     3661 tttgcggata ctccttcaac accgattcac gcaccactgg tgcaggcgcg tagataggtt
     3721 gcacgccttg cggatcgctt aaggtttgca gccccagcgc cgcgaccgga ccgtcagtgc
     3781 cgtaagccat tgcggcatta acgccagagg tttgctgggc agcggcttta atcgtcaccg
     3841 ccgtgtcgcc accagccagt gacagcaact gatcctgacc gagcttaaag ccataggctt
     3901 tttcaaacgt gggtaacgca tcggcgcgtt cgataaactc tgccgaggct gccagtttga
     3961 aggtgccgcc ctcttgcaga taacgactca ggtcggcaag cgaagtgagt ttgtttttct
     4021 ctgccacatc ctgacgcacg gcgatagtcc aggtgttatt tgcaggcgcg ggcgtcagcc
     4081 agattaactt gttgtgctcc gcatcgagct ttttgacttt ctcgtagccc tgcccggcat
     4141 ttttccacgc tgcatcgttt tcatctttaa agaagaaagc gccattgccg gtatattccg
     4201 gatagatatc cagttcatcg gaagtaatcg ccccgcgccc cacaggagtc gttccgagtt
     4261 gcactttatt caccgtcggt acgccgtggc tttcgagtac ctgcaagatg atgttgccga
     4321 gcagcgcgcc ttcggtatcg atttttgaac cgactttaac gggggaagcc gcctgtagcg
     4381 gcaggctcac ggctgccaac ataaccagtg aacctgccca gagctttaag agtggcatga
     4441 tgctttcctc attcttttac tgttgttttc agcgaattaa gagaaaagca tagttgataa
     4501 tgacagagtt agcctgagcg gaaggattca gttgcagaat cagataaata cttaagaggc
     4561 atattcggtc cggcgtttcc tgccggatgc ggcgcgagcg ccttatccgg cctacaaagg
     4621 gcgaaaattc agcaagttaa gatatgaaca tcaggcaaag ggcgcaaacg tcgcgccctt
     4681 actaaagcat tacagcaact caaactcgcc tttcttaacg cgtgcggaat cagtgccgat
     4741 aaatacattg aacttgccag gctcggcgtc atatttcatc tgttgattcc agaacttcag
     4801 cgcctcaata tcgatcggga agctgacagt ctgagtttcg cccggtttca gggtgatttt
     4861 ctcaaagcct ttcagctgtt tcacagggcg actcatggaa gccgtcacat cctgcaagta
     4921 catctgcact accgtggccc cctcgcgctt accggtgttc gtcacctgca cgctggccgt
     4981 cactttgccg tcacgcttca tggtcggcgc agaaagtttc acatcagaga cggtgaaagt
     5041 ggtatagctc agaccatagc cgaacggata aagcgctccg ttagcttcat caaaataacg
     5101 cgaagtatat ttgttcggct tgtcggcatt atacggacga ccggtgttca gatggctgta
     5161 gtacaccggg atctgcccga cagaacgcgg gaaggaaatt ggcagcttgc cggacgggtt
     5221 gtaatcgcca aacaatacat cggcaattgc attaccgcct tcagtccccg caaaccaggt
     5281 ttccagaatc gcatcagcct gctgatcttc tttcaccagc gccagcggac gcccgttcat
     5341 cagcaccagc accagcggtt taccggtggc tttcagcgcc gcaatcaagt cacgttggcc
     5401 ttgcggaata gtgatatcgg tccggctgga ggcttcgtgc gccatcccct gtgcttcacc
     5461 gactacagcc accaccacat cagattgttt cgccgtctgc accgcttcat caatcatctc
     5521 ttgcggcgaa cgcggatcga ctttgaccgc ttcttcatac tgattcagga aatcgataat
     5581 gcctttgtca ctggtaacgt tcgccccttt ggcatacagc actttaccgt tttcaccgac
     5641 ggaattttta atcccggtca gtacggtcac ggattgatcg gcaacaccgg ctgcggacca
     5701 gctgcccatc acgtcacgtt tactgtccgc cagtggccca accaccgcaa tggtggccga
     5761 ttttttcagc ggtaacgttt cgagacggtt tttcagcaac accaggcttt cgcgcgccac
     5821 ttcacgcgct tctttacggt gcaggcggct ttcggcattg gtatccaccg ggtcagactc
     5881 tttcggcccc aaatggctgt atgggtcgtt aaacaacccc atatcatatt taacgttcag
     5941 tacatggcgg gcagcatcgt ccagctctgc catggtcact ttgccggatt taatcaaccc
     6001 aggcagatac ttcgagtagt actcgtcgct catactcatg ttgattccgg atttcagcgc
     6061 cacgcgcacc gcatcttccg ggtctgccgc cgtgccatgt ttaatcagct ctttgattgc
     6121 accgtgatcg gaaacggtga tgcctttaaa gccccactgg tcgcgcagaa catctttcag
     6181 cagccaggaa tcggaggtgg ctggcgtgcc gttcagcgag ttcagcgcca ccatcaccgc
     6241 gccgctgcct gcgtccagcc ccgctttgta cggcggcata taatcattaa acaggcgctg
     6301 cggactcata tcgacggtgt tgtactcttt accgccttct accgcgccgt atgcggcaaa
     6361 gtgtttgacg ctggtcatca ccgagtaacg atctgccggg cttttaccct gcatcgcttc
     6421 caccatggtt ttacccatta ttgaggtgag atacgtatct tcgccaaaac cttcggaagc
     6481 acgtccccag cgcggatcgc gcgagacatc gaccatcggt gcccaggtca tattcaggcc
     6541 atcatctgcc gcttcataag cagagacacg cccgaccgtt ttcaccgcat cgaggttaaa
     6601 agacgaggcc agaccgaggc taatcgggaa caccgtgcgc tgaccgtgca gcacgtcgta
     6661 agcaaagaaa agaggaattt tcaggcggct taattccatc acctgatcct gcatggcgcg
     6721 gatatcctga cgggttacgg tgttgaaaat cgccccaacc tgaccgtctt tgatcatctc
     6781 gcggatcgcc tctttcggat tatccgggcc tacgctgatt aagcgcagct gaccaatttt
     6841 ctcatcaacc gtcattttct tgagcagatc ggtgacgaac gcatcccgcg cttcaggcgt
     6901 taatggatgg ttaccgaata aatcatccgc cagtgctggc tgcaggacca gactcaccgc
     6961 gattcctact gaacatagcc atttcatatg gatttttctt cctttgttgc ccgacgtggc
     7021 agcgaaaatg gtgcaaaaac cgtagtttgc cataagcatg atggagagag aaaaagaatg
     7081 ctcagtttat tgtctgaatt ttcaaaatat tcactcgctg aattgtcata caaggcgcta
     7141 ttctagtttg tgatattttt tcgccaccac aaggagtgga aaatgtcttc catgacaaca
     7201 actgataata aagccttttt gaatgaactt gcccgtctgg tcggtcattc acacctgctc
     7261 accgatcccg caaaaacggc ccgctatcgc aagggcttcc gttctggtca gggcgacgcg
     7321 ctggctgtcg ttttccctgg ctcactactg gaattgtggc gggtgctgaa agcctgcgtc
     7381 accgccgaca aaattattct gatgcaggct gccaatacag gcctgaccga aggatcgacg
     7441 ccaaacggta acgactatga tcgcgatatc gtgatcatca gcaccctgcg tctcgacaag
     7501 ctgcacgttc tcggcaaggg cgaacaggtg ctggcctatc cgggcaccac gctctattcg
     7561 ctggaaaaag ccctcaaacc gctgggacgc gaaccgcatt cggtgattgg atcatcgtgt
     7621 ataggcgcat cggtcatcgg cggtatttgt aacaactccg gcggctcgct ggtgcaacgt
     7681 ggcccggcgt ataccgaaat gtcgttattc gcgcgtatag atgaagatgg caaactgacg
     7741 ctggtgaacc atctggggat tgatctgggc gaaacgccgg agcagatcct tagcaagctg
     7801 gatgacgatc gcatcaaaga tgacgatgtg cgtcacgatg gtcgtcacgc ccacgattat
     7861 gactatgtcc accgcgttcg tgatattgaa gccgacacgc ccgcacgtta taacgccgat
     7921 ccggatcggt tatttgaatc ttctggttgc gccgggaagc tggccgtctt tgcggtacgt
     7981 cttgatacct tcgaagcgga aaaaaatcag caggtgtttt atatcggcac caaccagccg
     8041 gaagtgctga ccgaaatccg ccgtcatatt ctggctaact tcgaaaatct gccggttgcc
     8101 ggggaatata tgcaccggga tatctacgat attgcggaaa aatacggcaa agacaccttc
     8161 ctgatgattg ataagttagg caccgacaag atgccgttct tctttaatct caagggacgc
     8221 accgatgcga tgctggagaa agtgaaattc ttccgtccgc attttactga ccgtgcaatg
     8281 caaaaattcg gtcacctgtt ccccagccat ttaccgccgc gcatgaaaaa ctggcgcgat
     8341 aaatacgagc atcatctgct gttaaaaatg gcaggcgatg gcgtcggtga agccaaatcg
     8401 tggctggtgg attatttcaa acaggccgag ggcgatttct ttgtctgtac gccggaggaa
     8461 ggtagcaaag cgtttttaca ccgtttcgcc gctgcgggcg cagcaattcg ttatcaggcg
     8521 gtgcattccg atgaagtcga agacattctg gcactggata tcgctctgcg gcgtaacgac
     8581 accgagtggt atgagcattt accgccggag atcgacagcc agctggtgca caagctctat
     8641 tacggccatt ttatgtgcta tgtcttccat caggattaca tagtgaaaaa aggcgtggat
     8701 gtgcatgcgt taaaagaaca gatgctggaa ctgctacagc agcgcggcgc gcagtaccct
     8761 gccgagcata acgtcggtca tttgtataaa gcaccggaga cgttgcagaa gttctatcgc
     8821 gagaatgatc cgaccaatag catgaatccg gggatcggta aaaccagtaa acggaaaaac
     8881 tggcaggaag tggagtaaaa attacggatg gcagagtatc gccatccgaa ttcacttaat
     8941 cgttctgtgc cgtctgcccc gccgccgcca tttgggcggc tttttgtttt ttatagctca
     9001 acgctgctgc cggcacaggc atcactttac cggtttcaat ccaggtacgc aggcggctgg
     9061 catcggcaaa atgggtatat ttgccaaacg cgtccatcac taccagcgcc accggtttat
     9121 tattgataac cgtacgcatc accagacaat ggcccgccgc attggtaaag ccggttttgg
     9181 ttaactgaat attccagtta tcgcgataca ccagatgatt agtattgcgg aacggcagcg
     9241 tatacgtcgg gttagagaag gttgccatat cttcccgggt agtacttaac tgcccgatca
     9301 acggatattg tttgctggca atgagcagtt tggttaagtc acgggcagtt gaaacgttat
     9361 gcaccgacaa tccggtaggt tcaacaaagc gcgtgttgtt cattccgagc gatttcgctt
     9421 tcgcattcat tgccttaata aaggctttgt aaccaccggg ataatggtgc gcaaggcttg
     9481 ccgccgcgcg gttttctgaa gacatcagcg ccagcaacag catatcttta cggctgattt
     9541 cgctattcag tcgtacgcgc gaatagaccc ctttcatctc cggcgtctgg ctgatatcca
     9601 cttttagttt ttcatccagc ggcagtcgtg catccagcac aaccatcgcg gtcattaatt
     9661 tgctgataga cgcaatcgga cgcaccagat ccgggtggtt cgaatagatc actttgttgg
     9721 tattcagatc aacaatcatc gcgctaccgg aggcaatttc cggttgtgaa gcggtggtag
     9781 cggctgccgt tttcgcaacg gcctgcggtg caaaaggcac agccagcatc agggccaagc
     9841 taaataaaga aactcgaaat ttcggcatga tgagcattca gatagtggtt cacgcgcacg
     9901 ggttgcgcac cgccggagta aggatttact gaggctagcg acgccatcat aacgagcaaa
     9961 aagtgcgatc gtcaaaggag aatcgtgagt aaatgctgca ttgctgacat ttacgccagc
    10021 aatgcaacgt caaagaactt tctagaacaa acgataaccg tagccccata gtataacggt
    10081 tagggtgagc agcacttcca gtaccagcac gccaatcgcc agcgtcgaac tggagaagct
    10141 aaggccttcc tctttgttga tattcaggaa gctcggaata ccaaggtaaa gcaggtagcc
    10201 ggtgtaaaac agcgccaccg tgccgaccag cgcacacaac cagaccagtg gataaagcgc
    10261 caccagaccg cttaaaaaca gcggagttgc aacgtagccc gcgaagacca tgcagtgcgc
    10321 aagagacgga cgctgcgggt aattgcgcgc catccaccag atgacccgcc ccatcaccgc
    10381 aaccccagcc agcataacgc cgtaaaacaa gacagccagc gccagtccgg taaaccagga
    10441 taacttcagg atagtgccat cgccaaaatt ccagccaatc tgtgtagtgc caatgaaggc
    10501 gcaaatcacc ggaatcgccg ccatcagcaa aacgtggtgg gtgtaatggt gagaaatcgt
    10561 ttcgttttcg cgattaatca cctgcatttc acgatcggga tgggaaaaca gtccccagac
    10621 atggctcata ccgccccctt gttgtgagtt catgaacctg acagttcaag tataagccag
    10681 cttgtgatta ttttttgttc atccatgtaa tttgcaccgt ctgatgtatg tcctttttca
    10741 gcgtgaatga ttcacagggt gtatgcttac aggcaaccaa agggagacaa actggcctat
    10801 ggatctcaat acactcatct cacaatatgg ttatgccgcg ctggttatcg gtagcctggc
    10861 ggaaggtgaa accgtgactt tgctgggagg cgttgcggca catcaggggc tattaaagtt
    10921 cccactggtg gtactttctg tggcgcttgg tggcatgatt ggcgaccagg tgctctatct
    10981 gtgcgggcgg cggtttggcg gcaagctgtt acgccgtttc tcgaaacatc aggataaaat
    11041 tgagcgggca cagaaactta tccaacgcca tccgtatctg tttgtcattg gtacgcgctt
    11101 tatgtatggc tttcgggtga ttggcccgac gctgattggt gccagccagc tgccgccgaa
    11161 aatctttctg ccgctgaata ttctcggcgc atttgcctgg gcgttgattt ttaccactat
    11221 tggttacgct ggtggtcagg tgattgcgcc gtggttgcac aatctcgacc agcatttgaa
    11281 gcactgggtc tggttgattc tggtcgtggt tctggtggtg ggcgtgcgct ggtggctgaa
    11341 acggcgcggg aagaaaaagc cggataatca ggcgtaaagc agttgccaca gataagcatt
    11401 cactccacat ccggcaccgg gtgttccatg cctgatgcga cgctggtgtc ttatctggcc
    11461 taacccattg aaatatccac gcacctcact gcgctattct gggttgaact gcggattcgc
    11521 caacataaag ccgccatcca ctatcaacga ctgccctgtc gtgtaatttg cgccctccga
    11581 acaaagccac accaccaggc tggcaatctc atgcgttgcg ccaaaacgcc gtagaggaat
    11641 cgaaggctcc gcgtcgggct tcacgtcgct atcatccatg ctgttcattg gcgtggctat
    11701 cgccccaggc gcgactgcgt tcaccaaaat cttatgcctg accaactcca gcgccatcgt
    11761 tttggttaac ccgccgagcg catgtttagc ggctgtgtag gcgctggcat ccggcagcgg
    11821 cgtatgttca tgtaccgacg taatgttgat gatgcgaccg ccctgccctt atttcaccat
    11881 ctgacgagcc gcaatttgcg agcataaaaa tgcaccatca acatcaacgg taaaaatctt
    11941 gcgccactca tcaaaagcca tatcaagaaa cggcgctttg gtcattgcac ccgcattatt
    12001 caccagcaca tcaatacgcc caaatcgctg gatgagtgtc tccagcgcct gcgcaccttc
    12061 tggcagattg ccgagatcca gttgcacgat ctccgcacgt acgccgtggg taaccacctc
    12121 acgcgcggta tcttttgccc cttcttcatc tgagtgccag gtaatgccaa tatcaaaccc
    12181 ctgctgcgcc agtaataacg cgcactcttt gccgatcccc gaatcggaag cggtaattat
    12241 cgcgacctat gccatcgagt tctccactta acgctgaata aacgttaagt atagaaggcg
    12301 catatcatca gcgtttgtaa cccccgccca gcgcaccggt gagtcgaatg gaggcatcca
    12361 gccactgccc ttgcaataac aggccattgg cccgctcacg cagcgccggg attctggctt
    12421 cgctgacgcg ggaaccagca atgatgcccg cgttaaagcg ggcctgcgca agacctacca
    12481 cacgcaaggc atcgcgctca atttgcgcct gatgctggtt tttctccgcc agtgtctgaa
    12541 cctgactggc tgcccgcgcc acgtcattca ctgcttcaac caccgctttg ttgtagctgg
    12601 cgatagacaa gttgctttcg gcttttgcga tatcgagatt ggcgttaaga cgaccactat
    12661 cgaaaatagg tagcgtcagg cctgccgtaa cgcccatttg ctgcgcggaa tgacggaaca
    12721 gatcgcttaa gtgcaacgca tcctgttgca ggaaggccat caggttgatg tcaggataaa
    12781 atgccgcttt tgccgcatca atagtgctta acgatgactc aacgtaccag tgcgccgcct
    12841 gcaaatctgc ccgccgggcc agtaaggagt accccagttc atcaggaagc tggcttgcca
    12901 ctttcggcaa cgcgaccggt ttaagcttca atgacttagt ctggtgattt gtgagtgcat
    12961 ttaaccgtgc ctcaataatt ttcattttcc ccgcgacatc gttgagctgc tgccgggttt
    13021 tgctggcatt aatatcggtt tccacaccgt caactgaaga agtaatcccg ttctgatata
    13081 gctggcgatc ggtcgcgata atggtgttct gctctttttc tatttgctgc aagaccgtgt
    13141 ttaacgccgc ctgggtttgc cactcccagt acaggcgggc tacgctgcca gccagcaatt
    13201 ggcgggtttg ctcgcgttcc gccgcccgtg ctttaaccgt acccaggcgg gcagtaacct
    13261 ccgcccgatt ctttccccag atatcgagat gccagcccgc cgttaagcca aaagttccgt
    13321 tggtgtacca cggaccggtc gtacctgcgg ccggatcgtt cagagcaaac ggccccatta
    13381 gcccttctgc cgacattttt tgccgctcca tatccgccga aaagtcgatc tgcggaccat
    13441 cctgagtggc aactgccttc gcctgggctt cagctagcta aatgcgctgt tcagccacct
    13501 gcatatccgg tgcgttctgt agtgcattgt taattaatga agtgagctga ttatcgtgat
    13561 actccagcca ccattggctg tctggccaac catttttcag cgccgtgggt aatgcggtgt
    13621 caacttgtgc agcgggcgtt tgctggctta acgcctggcg ggtttcatgc ataggcgcac
    13681 acccggccag catcagtaac agcggaaaac aggcgatggc tggataaaag gaatcacgat
    13741 tcatggggga ataatcaggt aagaaacggt gcgcggagat taccgtgtgt tgcgatatat
    13801 tttttagttt cgcgtggcaa tacatcagta gcaataaaac gacataccca gaaaaatata
    13861 gactaagtaa atgatatctt ccgatttatc ttaatcgttt atggataacg gcaaagggct
    13921 tcgttttttc ctatacttat tcagcactca caaataaagg aacgccaatg aaaattatac
    13981 tctgggctgt attgattatt ttcctgattg ggctactggt ggtgactggc gtatttaaga
    14041 tgatatttta aaattaatta atgtcatcag atccgaaaat aacgagaata tttcagtctc
    14101 tcatcctgtt gcgctcctgt catgtgcatt gcttcatata atcactggcg caaggagcgc
    14161 gcagggggcg accaatcgcc gccgccccct gcccccccgg gctctggcga acaaaatcgc
    14221 cgctgcgcgg tgccctcggc ttatccctta cggctaccgg gtcgggcgcg aggtaacatc
    14281 cctgtaaaac gcgccctcag cccacatcca tgtgggctgc cccggccttc agggaacgcc
    14341 tcggcgattt tgacgccacc aaacaaccgt gcggcctatt gataaagagc taacacattg
    14401 tcaaaaacat cactatggtt tttagagttt ctcgatatca attgcctgaa tagcccttgc
    14461 aatatcaggg gagttattca acacccgaac atgctgaaat aattccgttg cttcatcgta
    14521 ttctttacgc aaataactca accactgttt aatccgcgca acgtgatata acccggtatc
    14581 gccctgcttt tccagacggg tatatttttg cagcaaagca accacctccg gccacggcat
    14641 tcgcggttcg ttatatttta ccacccggct caggttggga atattgagcg ccccgcgacc
    14701 aatcatcact gcgtcgcagc cgctgatcgc catgcattgt tgcgcgctct gccagtccca
    14761 gatttcaccg ttggcaatca ccggaatatt cagccgctgg cgaatctcgc caatcgcctg
    14821 ccagtcaata tgctccgcgc ggtaaccctg ctctttcgtc cgcccatgca ccaccagctc
    14881 cgtagcgcca gcctgttgaa ccgcatcggc gatttcaaat ttcttctcac cgctgtccca
    14941 gcccagacgc actttcacgc tgacaggcaa atgcgccggt acagcttcac gcatcgcttt
    15001 tgccccctgg tagatgagtt caggatcttt gagtaacgtc gccccgccgc cgctaccgtt
    15061 aaccgttttc gacgggcagc cgcaattgag atccacgccc cagaaaccta actccaccgc
    15121 acgggcggcg ttctctgcca gccattgtgg gaactgacct aacaactgca cgcgcaccag
    15181 cgtaccagat ggtgtccggc tggcgttttg tagctcaggg caaatgcgat gaaagacttt
    15241 taccggcagc agttgatcca ccacgcggac aaactcggtg atgcacagat cgtagtcgtt
    15301 aacttcggtc agcaattcac gcaccagaga gtcaagcact ccctccatcg gtgccagtaa
    15361 cacacgcata tcatcacccg caaaaaaatg aggcgctatg ttagcgccta tggtcagcgg
    15421 attaaaggtc tgcaaagagg gattgctgca aactgcaatc cctctctatg cttgatgtat
    15481 gcctgagttc gctgctcatt gcgtaatacc gggtagcgaa aaaataagcg ctgatttctt
    15541 aatgtgatcg gtagcacgtt ttacgaatta attgtatgat gaatccatct catctggggt
    15601 gttgattatg agtaagacac tgaacattat ctggcaatat ttacgcgctt tcgtcctgat
    15661 ttatgcctgc ctgtatgcag gcattttcat tgcttccctg ctaccggtaa ccattccggg
    15721 cagcatcatc gggatgctga tcctgtttgt cctgctggcc ttgcaaattc ttccggcgaa
    15781 atgggtcaat ccggggtgct acgtactgat tcgctatatg gcgctattgt ttgtgccgat
    15841 tggcgtaggc gtcatgcaat attttgattt gctccgcgcg cagtttggcc cggtagtggt
    15901 ttcctgtgct gtcagtacgc tggtggtttt tctggtggtg agctggagtt cacaactggt
    15961 acacggtgaa cgtaaagtcg taggtcagaa aggatcagaa gaatgatggc gaatatctgg
    16021 tggtcattac cgctgacgtt gattgtcttt tttgccgccc gcaaactggc ggcacggtat
    16081 aagtttcccc tgcttaaccc gttgctggta gcaatggtgg tgatcattcc ttttttaatg
    16141 ctgaccggca tctcttacga cagttacttt aaaggtagcg aagtgctaaa cgatctgctg
    16201 caaccggcgg tcgtcgcgct ggcctatcct ttatatgagc agctacacca gatccgcgcg
    16261 cgctggaaat cgatcatcac catctgtttt atcggcagcg tggttgcgat ggtgacgggg
    16321 acttccgtgg cattgttgat gggcgcttca ccggaaatcg ccgcgtcaat cctgccgaaa
    16381 tcagtcacca cgcctattgc aatggcggtt ggcggcagta ttggcggtat tccggcaatc
    16441 agcgcagttt gcgtgatttt cgtcggcatc ctcggcgcgg tatttggcca tacattgctt
    16501 aatgcgatgc gtattcgtac caaagctgcg cgcggtctgg caatggggac tgcctcgcac
    16561 gccctcggta cggcgcgctg cgccgagctg gattatcagg aaggtgcatt tagttcgcta
    16621 gcgctggtgt tatgcgggat aattacttcg ctgatcgcac cgttcctttt cccgattatt
    16681 ctggcagtaa tgggctaaaa tttgcgatac gtcgcgcatt tttgatgtaa gtttcacgtg
    16741 ttgcataatt aatgagattc agatcacata taaagccata acgggttcgt aaactgttat
    16801 cccattacat gattatgagg caacgccatg catccacgtt ttcaaaccgc ttttgcccaa
    16861 cttgcggata acttgcaatc tgcactggaa cctattctgg cagacaagta cttccccgct
    16921 ttgttgaccg gggagcaagt ctcatcgctg aagagcgcaa cgggactgga cgaagacgcg
    16981 ctggcattcg cactacttcc gctggcggcg gcctgtgcgc gtacgccatt gtcgaatttt
    17041 aatgttggcg caattgcgcg cggtgtgagc ggaacctggt attttggtgc caatatggaa
    17101 tttattggtg caacaatgca gcaaaccgtt catgccgaac aaagcgcgat cagccacgct
    17161 tggttgagtg gtgaaaaagc gcttgcagcc atcaccgtta actatacgcc ttgtggtcac
    17221 tgccgtcagt ttatgaatga actgaacagc ggtctggatc tgcgtattca tttgccgggc
    17281 cgcgaggcac acgcgctgcg tgactatctg ccggatgcct ttgggccgaa agatctggag
    17341 attaaaacgc tgctgatgga cgagcaggat cacggctatg cgctgactgg tgatgcgctt
    17401 tctcaggcgg cgattgcagc ggcaaaccgt tcgcacatgc cttacagtaa gtcaccaagc
    17461 ggtgtcgcgc tggaatgtaa agacggtcgt attttcagcg gcagctacgc tgaaaacgcc
    17521 gcattcaacc cgactctgcc gccgttgcag ggggcgttaa ttctgttgaa tctcaagggt
    17581 tatgattacc cggatatcca gcgcgcggtt ctggcagaaa aagccgatgc gccgttgat
//
LOCUS       DAJTSI010000082        17397 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000082 DAJTSI010000000
VERSION     DAJTSI010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17397
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.082"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..956)
                     /locus_tag="OO327_002484"
     CDS             complement(<1..956)
                     /locus_tag="OO327_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR6018990.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGS"
     gene            1202..1828
                     /gene="gstB"
                     /locus_tag="OO327_002485"
     CDS             1202..1828
                     /gene="gstB"
                     /locus_tag="OO327_002485"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HCR6018991.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="OO327_002486"
                     /pseudo
     CDS             complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="OO327_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="OO327_002487"
     CDS             complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="OO327_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR6018992.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3646..4971
                     /gene="rimO"
                     /locus_tag="OO327_002488"
     CDS             3646..4971
                     /gene="rimO"
                     /locus_tag="OO327_002488"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR6018993.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLMSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5018..6346)
                     /locus_tag="OO327_002489"
     CDS             complement(5018..6346)
                     /locus_tag="OO327_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6018994.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6354..8696)
                     /locus_tag="OO327_002490"
                     /pseudo
     CDS             complement(6354..8696)
                     /locus_tag="OO327_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(8874..9785)
                     /gene="gsiD"
                     /locus_tag="OO327_002491"
     CDS             complement(8874..9785)
                     /gene="gsiD"
                     /locus_tag="OO327_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR6018995.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9788..10708)
                     /gene="gsiC"
                     /locus_tag="OO327_002492"
     CDS             complement(9788..10708)
                     /gene="gsiC"
                     /locus_tag="OO327_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR6018996.1"
                     /translation="MLYYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10726..12264)
                     /gene="gsiB"
                     /locus_tag="OO327_002493"
     CDS             complement(10726..12264)
                     /gene="gsiB"
                     /locus_tag="OO327_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR6018997.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12284..14155)
                     /gene="gsiA"
                     /locus_tag="OO327_002494"
     CDS             complement(12284..14155)
                     /gene="gsiA"
                     /locus_tag="OO327_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR6018998.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14142..15107)
                     /gene="iaaA"
                     /locus_tag="OO327_002495"
     CDS             complement(14142..15107)
                     /gene="iaaA"
                     /locus_tag="OO327_002495"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR6018999.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15311..16546
                     /gene="moeA"
                     /locus_tag="OO327_002496"
     CDS             15311..16546
                     /gene="moeA"
                     /locus_tag="OO327_002496"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR6019000.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            16546..17295
                     /gene="moeB"
                     /locus_tag="OO327_002497"
     CDS             16546..17295
                     /gene="moeB"
                     /locus_tag="OO327_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR6019001.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
BASE COUNT         4426 a         4640 c         4114 g         4217 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgatcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattgctgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atctgccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcaggatc cagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaatacg ttatatcgcc gtatgataag ttcggtgatt
     1021 aataacgcac acacaaactt aaagaaaccg taagttggta aagtttttaa agtttatgca
     1081 atatcctgcc gcttcgctgc ttctggtgtc atttttttgc cagctgttgc ttaaaagtta
     1141 actttacttc accatcggag tcctgttcag tctgtggctg cctgtcggaa aaggggtgag
     1201 tatgattacg ctgtggggtc ggaataattc aactaacgta aaaaaagtat tgctgacgct
     1261 cgaagaactg gaactacctt atgagcaaat tctcgcgggc cgtgagtttg ggataaatca
     1321 cgatgctgat tttctggcga tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga
     1381 aagtgatctc attctttggg aatcaaacgc cattgtccgc tatctggcgg cacagtacgg
     1441 gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa aatggatgga
     1501 ctgggcaaac cagacgctca gtaatgctca tcgcgggatc ctgatgggat tagtcagaac
     1561 accaccggaa gagcgcgatc aggccgccat tgatgccagt tgcaaagagt gcgacgccct
     1621 gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg acgagtttgg
     1681 tgtgggcgat atcgctatcg caccgttcat ctacaatttg tttaacgttg gcctgacctg
     1741 gacaccgcgt ccaaatctgc aacgctggta tcagcaactc actgaacgcc ccgcagtgcg
     1801 caaagtggtg atgattcccg ttagctaatt gcgtgggcta actttaagta attccccact
     1861 ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat cacgaattcg
     1921 ctgccctctg tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt tgacgctcat
     1981 cacaatgaca tctttatctt tcagctccca ataaataatt tttgctgcca ctgggggaat
     2041 ttatcgctgt tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca
     2101 ggttgctcgg tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag
     2161 ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt
     2221 tcatcaccac cgcgcgggcc atgttcattc agccacagtg cattactcca cggattcatc
     2281 gccattcctt gcggattacg aatgccataa gaccagatct cggcgcgcgc accggattcc
     2341 tttataaaag gattatcatc cgggatttcg ccctggtcgg tcagacgcac cagtttgccc
     2401 tgtaatttat ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa
     2461 agataacctt taccgtcgaa taccagccgc ccgccaaaat ggttgccggt agacagtttt
     2521 ggcatctggc ggaagacggt gcggaagtcg gtcacttttg agagatcatc acttaagcgg
     2581 ccataaccca cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag
     2641 atgcggcgag actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg
     2701 tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata atccttttcc tgcttgccag
     2761 tgacgcaact cgccgccgcg cagagtgatt aacataccgt gattatcggg taaaaaggcc
     2821 agtgcccagg gatggtcgag tttgtcttgc agtacttcga catttaccgt tgcaggagcc
     2881 gcccagagag cggaagaaag acaaataagg ggcacaagga aaaaggattg tcgatgcata
     2941 acgcctccgg attgactctg gcttaaagcg tagtcagtgg aggagataac aaattcattt
     3001 ttacaaaaac ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct
     3061 caagcatggc gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt
     3121 caagcttatc ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac
     3181 atactgcggc tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat
     3241 ccagatgaag attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt
     3301 tatctcgtag atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca
     3361 cgtatgcagc gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc
     3421 tgtcaacgaa catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc
     3481 tattttttga gcaaaatgct gtccaggatc aattcttcac gccgaattaa caaaatttac
     3541 atttccttcg cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta
     3601 gaatccctgc cctgaccttt cctgtaactg aatatttttt aagctatgag caaagtaact
     3661 ccccagccga aaatcggctt tgtttccctt ggctgtccga aaaaccttgt cgattcagag
     3721 cgtattctca ccgaactccg cactgaaggt tatgacgtgg taccgagcta tgacgatgcg
     3781 aacatggtga tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa
     3841 gccattggtg aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg tctgggggca
     3901 aaagaagatc agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc
     3961 tatgagcagg ttctggagca cgttcatcac tacgtgccaa aaccgaaaca caacccattc
     4021 ctgagcctgg tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa
     4081 atttcggaag gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac
     4141 ctgatgagcc gtccgattgg cgaagtgtta agtgaagcga aacgtctggt agatgcgggc
     4201 gttaaagaga ttctggtgat ctcgcaggat acttccgcct atggcgttga tgttaaacat
     4261 cgtactggct tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag
     4321 ttatcgaaac tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac
     4381 gacgtcatcc cactgatggc agaaggcaaa atcctgccgt atctggacat tccgttgcag
     4441 cacgccagcc cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactg
     4501 gcgcgcatca aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt
     4561 gtcggcttcc ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa
     4621 gcacgtctgg atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat
     4681 gccctgcctg accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag
     4741 ctgcagcagc agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg
     4801 attatcgacg aagtggacga agaaggcgcg attggtcgca gcatggcaga tgcaccggaa
     4861 atcgacggcg cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt
     4921 gtgaaagtcg agcacgccga tgagtacgat ttgtggggta gccgggttta attaattcag
     4981 gccgggcaaa atcatttgtc cggcctgcga ataatgatta ctgccagcta atctgtgccc
     5041 tgccgttttt cttactctta tacagccgct catccgccag cattttcaag cgttcgacgg
     5101 tagtacattc atttgaatgc gctacaccga tactggtgct taaattaatg cttcccacct
     5161 ggaaatgaac gatttctttt agtatcgata gcaactcttc cagcgtgccg ccagtataaa
     5221 tcaccgcaaa ttcgtcacct ccgaagcggt agactttacc tttatctacc gggatctgtg
     5281 atttaaggat tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt
     5341 cgttaatgtt tttgaattta tcgacgtcaa acagacacaa ataaccgcct ttttccggca
     5401 gcgcttttaa atcttcatca aaggattttc tgcccattgc tttggtcagg tcgtccagga
     5461 acgccgagtt atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc
     5521 catccagtac gcgttcaaaa tggttattaa tgatggcttc tttctccaca ataccacgat
     5581 taatcactct gcgtaccaga tagaggcaac tggcacccaa tatcactgcc accaatgcgg
     5641 caaagaaaat aatcaatgta caaacccgca aataaacatc ttttttatac gcaaatacgt
     5701 acaatccggt cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg aagatggcgt
     5761 ttttagcatt atattcatca ccggtcaccg gtttaccgtt cttaaataga gagaacatca
     5821 ggttctcgct gtccatcacc agcgattttg acagtaaaga aaaacgctcc agaccaagca
     5881 aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt
     5941 caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg
     6001 cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag
     6061 gaccgacatc gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa
     6121 ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc
     6181 gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat
     6241 acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa
     6301 aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt aaactactct
     6361 tttactaatt ttccactttt atcccaagcg gagaatggca tgggtttctg gtacagatac
     6421 ccctgagcct gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg
     6481 ccttccgcaa tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt
     6541 tttcccgcag taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga
     6601 ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc
     6661 cgtacccctt gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg
     6721 atcacggttt cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg
     6781 atggtggtaa aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata
     6841 tgcagtaaga aatcatcctt cagcgacgca cgttgactaa aatgtctgaa ttaacccatt
     6901 gccgttttaa taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta
     6961 atgaacttca gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc
     7021 gcgctcagaa tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg
     7081 ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg
     7141 agtttcgcgt catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc
     7201 gccagtttca gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg
     7261 gcagacttat ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc
     7321 gctattttac gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca
     7381 ttgaatgtca cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc
     7441 ctgacgattt tattgcgttc atcaacacca aacaccgcct ccacggcttc cagaccacac
     7501 agtttgatga tccccacagc cagatactga ttattgttgg actcaataat cgccatattt
     7561 ttaataaaac cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca
     7621 atttcggcat ctttcatttc cgcgatcgaa tttttgatgc tgataatctc tttcgcatcg
     7681 ccttcgcgaa ttttcagagc ctcgatctga tccgttgagt cgggcaaaaa cgccagcgta
     7741 ttaaccagct cggtcatttc agaaaattgc cgacggaaca cacgagtggt catgaaatat
     7801 accaccagca acacgataat aatgaacgca aacggtagat aaaaaagttt atctgatgcg
     7861 ttcaataaat taccgccagg ataataaaaa tagatttcga acaatgcatt atttggcact
     7921 gctgatgtct ttgcaatata ctccgtatcg acagaatcaa taatattata attaccttcg
     7981 ctattataaa taatcagttt ttcacgagcg agttgattct tggcaatgat ctcatgacct
     8041 tgtttcaaaa atagaacttc atcattagcg ctgatttgat caagatactc gttcagatac
     8101 cgccagttta aatcgacatg aattttatct accgctaaca aatcgtgatc tttgccgttg
     8161 atacgtagcg taaaagagat acatttttta cccggacgat caaagcaatt gtatgccgga
     8221 ttccagctgt tgttagagtc atctgaagcc accgaccacg gtcgtttcga tgggttaaaa
     8281 tcggcaaaaa taacccgccc tgactcgtcg acaagcggtc catttacttg ttcctgatga
     8341 taaacaacga ttttatcatt cgccctccgc gaaaagagat aatatttatt atcatcaagt
     8401 accagtccca ccgagtctaa aaatggcatc atttccattt tcagaatcac ggcgcgttga
     8461 atctcctctg tattttttaa taaatacagt gagtccttta atgtcagggc cgaacgttca
     8521 atatctttaa agaaatagcc aacgtttttc tgaataagtt caaagcggct atcggcacgt
     8581 tttaccgcgc gttccgatac cagttgatag ttaatcatga aaaaaagacc aataaatgac
     8641 agtgctgcca ataaaaataa aattatcagc cttatctttt cgtataaact cagcatgtta
     8701 atacatttaa caaaataact atctgaacgc tgtgccgctg caaaaatagt cccttgctgc
     8761 ggctttttaa ccaaattacc cgattctaca aaatcagcag aaagttacaa cacttgctta
     8821 gggcaataat ttcctgtgag gtgattaccc tttcaagcaa tattcaaacg taactatcct
     8881 ttaattttcg gatccagcgc atcgcgtaaa ccatcgccca acaaattgaa cgccagtacg
     8941 gtcagaaaaa tagccagggc cggaaaaaca gcgacatgcg gcgcgataac catatccgct
     9001 cgtgcctcat tgagcattgc tccccactct ggtgtcggcg gctgcgcacc gaggccgaga
     9061 aatgacaggc tggcggcaga gataatcgag gtgccaatgc gcatggtgaa aaacaccacg
     9121 atagaagaga cggtcccagg caggatatga cgcaataaaa tggtcatatc gctggcacca
     9181 atactgcgtg ctgactcaat aaaggtttgc tgtttcaaca ccagcgtgtt gccgcgcacc
     9241 aggcgggcaa acgcggggat ggaaaaaatg gcgactgcga taatcacgtt agcaatgccg
     9301 cttcccaaca ccgcaacaac agcgatcgcc agtaaaatac ccgggaaggc aaacagcaca
     9361 tcgcaaatgc gcatgatcag ccgatcccac cagccttcat aatatccagc caacaagccc
     9421 agcaacgtcc ctatcgccac accgataaac acggcaaaca cgcccgccgc cagcgagatt
     9481 tgcgcaccaa ccaggacacg gctgaaaatg tcgcgcccca gtgaatcgac gccaaaccag
     9541 tgctgcaaag aaggtccgtt attcagattg tcataatcaa aataattttc ggcgtcatag
     9601 ggagcgatcc agcgtgcaaa aatggccacc acaatcaata aaataacgaa taatgcggcg
     9661 gtcatcgcca tatgctgacg gcgaaatcgt cgccagaatt catgccacgg tgtacgtacc
     9721 tggtcaggtt tgaccagtgg catggcgttt aacaccgcct gacgtcgcca gttaaatagt
     9781 cgcatcctta cttgtacctg atagccgggt taatggcggc gtaaagcaca tccaccacta
     9841 agttgataag aataaattcc agcgagaaaa gcagaatttc cgcctgaatc accgggtaat
     9901 cgcgcatttc tacagagtca acgagtaagc gtccaagtcc gggccagttg aaaactttct
     9961 ccaccacgat agaaccaccg agcaaaaagc caaactgtaa gcccatcatg gtcactaccg
    10021 ggatcatcgc gttgcgtagc ccgtgtttga ggacaaccca ggtttcgctc acccctttcg
    10081 ccctcgcggt acgcatataa tcttcgctta aaacgtcgac aaatgacgca cgggtaaagc
    10141 gcgccatcac ggcggccact gccgcgccga gcgtcaggga gggtaaaatg tagtgctgcc
    10201 aactgtctgc tcccacagta ggcagccagc ccagttcaac ggagaatacc tgaattaaaa
    10261 acatccccag cgcaaatgcc ggaaacgaga tccccgacac cgcaatggtc atactcaatc
    10321 gatccggcca acgattacgc cagacggcgg cgataattcc cgccgccata ccaaatatca
    10381 ccgcccagac catactggtt atggtcagcc atagcgttgg cataaagcgg ctggcaatct
    10441 catcggcaac cggacgacgc gacaccatcg acaggccaaa atccccctgc acagcattgc
    10501 tgatatagtg ccagaactgg tgatacagcg gctgatccaa ccccagctgc tgacgcacca
    10561 gttctataac ctgcgcatca gcttcgggcc cggcaatcaa tcgcgccgga tcgccgggca
    10621 gcatgtggac aaataaaaac accagcaccg agacgataaa cagcgtcgga atcaacccca
    10681 gtaatcgttt gataacgtaa taaagcattc cactccctgc gttgcttatt gcaaatccgc
    10741 gtcttcaaag ctgaagccgg tatctggcat gatccaaaaa ccggtcaggt ttttactgtg
    10801 tgccgacacc agtttttcta ccaccagcgg gatccacggc gattcttgcc agatgatatc
    10861 ctgcgccgcc ttatataagc gggtcttttc cgccggatca ttcgttttca gtgcctgagc
    10921 caggaagtca tccacctgtt tattgctgta aaacgcggta ttaaacagcg tcggtggcca
    10981 gttttgcgag gcaaacagcg gcgatagtgc ccagtcggct tcgccggttg aagccgacca
    11041 gccagtgtag aacatccgca cgccactctc tttttgccct ttaccttcaa cttctgccgc
    11101 ccgctgtccg gcatccatcg cggtcacctg ggctttaatc ccgacctgcg ctaactgctg
    11161 ctgggtaaat tgcagcactt tctgcgcggt gctgtggtta tgtgacgacc acagcgtggt
    11221 actgaaaccg ttgggatatc ccgcctcttt cagtaattcg cgcgctttca ctggatcgta
    11281 aggccacggt ttataacttt gcgcgtaggc gatacttggc ggtaccacac cagtagctgg
    11341 agttgcatag cccgcaaagg caactttcac cagcgccgga cggttaatgg cgtaattcag
    11401 cgcctcacgg accttcgggt tatcgaacgg cttttgcgtc acgttcatac tgatataacg
    11461 ctgcataatt gacggactgg ccatcaactc gatatttttg tttttctcca gcagtgtggc
    11521 ctgctcgtag ggaatgggga aagcaaactg cgcttcaccg gtttgcagca ttgccgcgcg
    11581 ggtgttgtta tccgccaccg gacgccaggt tatgctgtcc agtttgggca atcctggctg
    11641 ccagtaaccc gcgaattttt tcaccttcac aaaatcggtc tgattccagg tatccagttc
    11701 atacggtccg gttcccaccg gatgaaaacc aatctccttg ccatattttt ccagcgctgt
    11761 cggtgaaatc atcgcggtcg ccggatgggc aagaatatta ataaacgctg agaacggctg
    11821 tttgagggta atctttaccg ttgtcggatc gatcgcctcc gttttggcaa tattcttata
    11881 caggttatag cgtttaagat gattcgccgg atcgctggcc cggtccagat tcgcttttac
    11941 cgccactgcg ttgaaatcgg tgccatcctg gaatttaatt ccttcccgca atttcacggt
    12001 gtaagtaatg ccgtcatcgg aaacggtata actctccgcc agcacgtttt tcagtttcat
    12061 ctctttatcc agaccgaaca gcccctggta aaacgatttc gctacggcct gagataacgt
    12121 gtcattcgcg tcatacggat cgagcgtggt gaaattcgat cctaccgcca ccaccacatc
    12181 tttggcagcg aatgcacaag atgccatcaa cgctgtcgca atgcccagcg ccactaaccc
    12241 actacggtgt acagctcttg ccattttatt ctccgcctga atgttatcta cgcatgaatg
    12301 cgtattctga ttgtggttgt gcgacgtaat gccccggccc gacgcattgc aacgagacgg
    12361 ctgccacctc ttcgccacgc agatgaatat tgctgggaag atcgtccgac agcagtacac
    12421 gctgcggtcg ttgtcgggac ggttcagcga ccggaactgc cgccagtaat ttacgcgtat
    12481 aaggatgctg cgggttttcg aagaccgcgc accgtgaacc aatttcaaca atttgcccga
    12541 gatacatcac cgccacacga tgactaatcc gctctaccac agccatatcg tgggagataa
    12601 acagatacgc aatgccgaaa tcacgctgga gatcgagcaa caagttgata atctgcccgc
    12661 gaatagaaac atccagcgcc gaaacggctt cgtcggcaat gatcactttt ggattcaatg
    12721 ccaacgcgcg agcaatgcag atgcgctggc gctgaccgcc ggaaaactca tgcgggtaac
    12781 gccaggcatg ttcaggtaac aggcccacgc gctccagcaa ccacgcaacg cgtgcagccg
    12841 cgtctttacc tggcaataaa ccgtgtacac gcagcggttc gataatcgaa tcaccgatgg
    12901 tctgacgtgg gtccagcgaa gcgtaagggt cctgaaaaat aaactgaata tcccggcgca
    12961 atgcctgaag tttgccaggt gacaaggtat cgattcgctg accgttaaag ataatttcgc
    13021 cgccctgcga ttcgaccagg cgcagcaacg cccgcccggt agtggattta ccgctgccag
    13081 actcgcccac cagcgatagc gtttcgccag gccagagatc aaaactgact ttctcaacgg
    13141 catgcacttc ccgcgttacg cgattcaaca aaccgctgcg caaagggaaa cgggtgacaa
    13201 gattacgcac ccgtaaaaca ggttcgccat ccaccaccgt tttctgctcg atggggggtt
    13261 cctgtttcgc tggatgttca agcgatatca atgggaaacg tcggggataa tctaaccctt
    13321 tcatcgcacc aagttgcgga acagcagcta acagcgcacg ggtgtaagga tgttgcggtg
    13381 catgaaaaat ctgttcgacg gtacccgttt ccaccgcctc gccctgatac atcaccagta
    13441 cccgatcggc aatctctgcc accacgccca tatcgtgagt gataaagata acgcccatcg
    13501 acatctcttt ttgcaatact ttgattaatt gcaggatctg cgcctgaata gtgacatcca
    13561 gcgcggtggt tggctcatcg gcaatcagca ccgccgggcg gcatgacagc gccatcgcaa
    13621 tcatcactcg ctggcgcatc ccgccagaga gttgatgcga ataacgtgaa agaatggttt
    13681 gtgcctccgg aatgcgtacc tgatccagca tccgcttcgc ctcgaccatc gcttcttcac
    13741 gactggcgtt ctgatgcaga cgaattgatt cggcaatctg ttcacccaca gtaaatactg
    13801 ggttcagcga tgtcatcggc tcctgaaata tcatcgccat atccgcaccg cgcacatggc
    13861 gcatttgtgc agcgctctgc tcgctaagtt caatcacatc acgactgcgc cgccgcaaca
    13921 gcattttatc gcactgtact aaaccgcccg cctgttccaa cagacgcatc aatgccagcg
    13981 cagtcactga cttaccggag ccggattcgc ctacaattgc cagcgtctca ccgcgttgca
    14041 gactaaaaga gagattgcgg accgcagcta ttttctgctg gtcctgcata aaggcaatat
    14101 taagattttc aaccgccagc acattaccgg catcaagttc atcactgtgt ggcaacggtg
    14161 tccccttttt cacggtagat accggtggtt ggcgtatcgc ctgcgtagcc ccaggcgcga
    14221 tacattcctt cggtgttaaa cggtagcgcg acattccctt catggtcgat agcgattaag
    14281 ccaccgctac cgccaagcgc agggagtttt tccattacta cccgctcgca ggcttccgcg
    14341 agacttaatc cgccgtaatc cattaacgcg gcgatgtcat atgccgccag cgcgcggatg
    14401 aagacttcgc ctgtgccggt acaagaaacc gccacactgg cgttattggc gtagcatccg
    14461 gcacccacta agggactatc gccaactcgt ccgggtaatt tattggtcat tccgcctgtg
    14521 gacgtggctg ccgccagatt gccgtctaaa tccaacgcca cggcccccac ggtgcccatt
    14581 ttctgttttt catccagtgg cgcaccgcta tggtcgagga ctgttgcccc ttcctcgcgc
    14641 gctgccagta gttgttcata acgcaaaggc gtggagaaaa tctccggtga gacgcgctcc
    14701 atgccatgag caaacgcaaa attttctgcc ccttcgccaa tcatcatcac atgcgggctt
    14761 tgctccatca ccagccgggc ggcaagaacc ggattacgca gatgactaac gcccgccacc
    14821 gcaccggctt tcagggtgtt accatccatc acacaggcgt ccagttcatg ggtttcatca
    14881 cgcgtaaaga cagcgccaat tccggcgtta aacagtggac actcttccag cagacgcacc
    14941 gcttccgtca ccacatccag cgcactttcg ccggcttcca gcattttctg cccggtttca
    15001 acaatggcag acaacgcctc gatgtagcgt aattcctgtt gcagactcat ctgcgcgcgg
    15061 ctaattgcac ctgcgccacc atgaattgca atgactgctt tgcccatagt attcgtccat
    15121 taagggtggc tcgccacttt tgctataaat atcagaattt ttatgaatta ctcattcaat
    15181 ttttgaatat agaaagatgg atatggcatg taaaggcagg agccaacgct acgcccgagg
    15241 tggcatacag tctatggcgt ttttctgaca taataggcaa attcgatttt gcctccgcag
    15301 gagtgttttc atggaattta ccaccggatt gatgtcgctc gacaccgcgc ttaatgagat
    15361 gctttctcgc gttaccccac tgaccaccca ggaaacgctg ccactggttc agtgttttgg
    15421 tcgtattctg gcgagcgatg tcgtttcgcc actggatgtc ccggggtttg ataactccgc
    15481 tatggacggc tacgcggtgc gtttagccga tattgcctcc gggcaaccgc tgcccgttgc
    15541 cggtaaatcc tttgccggtc agccatacca tggtgaatgg cctgcgggta cctgcattcg
    15601 tattatgacc ggtgcgccgg tgccggaagg ctgcgaagcg gtggtgatgc aggagcagac
    15661 tgaacaaacg gacaatggcg tgcgttttac tgctgaagtg cgtagcgggc aaaatattcg
    15721 ccgtcgcggt gaagatatct ctgcaggtgc ggttgttttc ccggcgggaa ctcgcctgac
    15781 taccgcagag ctgccagtaa ttgcttcact ggggattgcc gaagttccgg tgattcgtaa
    15841 agtgcgtgta gcgctttttt ctaccggtga tgaactccag ttgcccggtc agccgctggg
    15901 cgacggccaa atctacgaca ccaaccgtct cgccgtacac ctgatgttag aacagctggg
    15961 ttgcgaggta atcaacttag ggattatccg cgacgatcca catgccctgc gcgccgcatt
    16021 tattgaagcc gacagccagg cggatgtagt gatcagttcc ggcggtgttt cagtgggtga
    16081 agcggattac accaaaatga ttcttgaaga gctgggggag atcgccttct ggaagctggc
    16141 gattaaacca ggtaaaccgt tcgcgttcgg taaactcagc aatagctggt tctgcggcct
    16201 gccgggcaac ccggtttcag cgacgctgac cttctatcaa ctggtacagc ctttgctggc
    16261 aaaactaagc ggcaacaccg ccagcggcct gcccgcgcgc cagcgcgtac gcacagcgtc
    16321 ccgcctgaaa aaaacgccag gacgtcttga tttccagcgc ggcgtgctgc aacgcaacgc
    16381 cgacggtgaa cttgaagtga cgaccaccgg acatcagggt tcacatatat ttagctcctt
    16441 tagtctcggc aactgcttta tcgtgctgga acgcgatcgc ggcaatgtgg acgtgcgcga
    16501 atgggtggaa gtagaaccgt ttaacgcgtt gttcggaggc ctgtaatggc ggaactcagc
    16561 gatcaggaga tgctgcgcta caaccggcaa atcattctgc gcggttttga ttttgacggt
    16621 caggaggcgc taaaagattc tcgtgtgctg gtagtcggcc tcggtggact cggctgcgca
    16681 gcttcgcagt atctggcaag cgccggtgtc ggcaacctga cgctgctcga cttcgacacg
    16741 gtttcgctct cgaatctgca acgccagaca ctgcacagtg atgccacggt cgggcaaccg
    16801 aaggtggaat ccgcccgtga cgccctgacg cggatcaacc cacatatcgc gattacgcca
    16861 gtcaatgcac tgctagatga cgcagaactt gcagcattga ttgctgaaca cgatctggtg
    16921 ctcgactgta cggataacgt tgcggtacgt aatcaactga acgcaggctg ttttgccgcg
    16981 aaggtgccgc tggtttccgg cgcggcaatt cgtatggaag ggcaaatcac ggtcttcact
    17041 tatcaggacg gcgaaccgtg ctatcgctgc cttagccgtt tgtttggcga gaacgcgtta
    17101 acctgcgtag aagcaggcgt gatggcaccg ttgataggcg taattggttc gttgcaggca
    17161 atggaagcga tcaaactgct ggcaggttat ggcaaaccgg ccagcgggaa aatcgtcatg
    17221 tacgatgcga tgacctgtca gtttcgcgaa atgaaactga tgcgcaatcc ggggtgtgag
    17281 gtgtgcgggc agtaattgcc acctccaagc ctgataagac gcgacaagcg tcgcatcagg
    17341 catttgctcc agacgccaga tgcggcgtga acgccttatc tggcctacgg ttcgggc
//
LOCUS       DAJTSI010000083        17386 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000083 DAJTSI010000000
VERSION     DAJTSI010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17386
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.083"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(42..773)
                     /gene="rlmB"
                     /locus_tag="OO327_002498"
     CDS             complement(42..773)
                     /gene="rlmB"
                     /locus_tag="OO327_002498"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR6019002.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(953..3394)
                     /gene="rnr"
                     /locus_tag="OO327_002499"
     CDS             complement(953..3394)
                     /gene="rnr"
                     /locus_tag="OO327_002499"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR6019003.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3433..3858)
                     /gene="nsrR"
                     /locus_tag="OO327_002500"
     CDS             complement(3433..3858)
                     /gene="nsrR"
                     /locus_tag="OO327_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR6019004.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4063..5361)
                     /gene="purA"
                     /locus_tag="OO327_002501"
     CDS             complement(4063..5361)
                     /gene="purA"
                     /locus_tag="OO327_002501"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR6019005.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5465..5662)
                     /locus_tag="OO327_002502"
     CDS             complement(5465..5662)
                     /locus_tag="OO327_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR6019006.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5744..6748)
                     /gene="hflC"
                     /locus_tag="OO327_002503"
     CDS             complement(5744..6748)
                     /gene="hflC"
                     /locus_tag="OO327_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR6019007.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6751..8010)
                     /gene="hflK"
                     /locus_tag="OO327_002504"
     CDS             complement(6751..8010)
                     /gene="hflK"
                     /locus_tag="OO327_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR6019008.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYSNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8096..9376)
                     /gene="hflX"
                     /locus_tag="OO327_002505"
     CDS             complement(8096..9376)
                     /gene="hflX"
                     /locus_tag="OO327_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR6019009.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9452..9760)
                     /gene="hfq"
                     /locus_tag="OO327_002506"
     CDS             complement(9452..9760)
                     /gene="hfq"
                     /locus_tag="OO327_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR6019010.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9846..10796)
                     /gene="miaA"
                     /locus_tag="OO327_002507"
     CDS             complement(9846..10796)
                     /gene="miaA"
                     /locus_tag="OO327_002507"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR6019011.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSV
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10789..12636)
                     /gene="mutL"
                     /locus_tag="OO327_002508"
     CDS             complement(10789..12636)
                     /gene="mutL"
                     /locus_tag="OO327_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR6019012.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12646..13983)
                     /gene="amiB"
                     /locus_tag="OO327_002509"
     CDS             complement(12646..13983)
                     /gene="amiB"
                     /locus_tag="OO327_002509"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR6019013.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14002..14463)
                     /gene="tsaE"
                     /locus_tag="OO327_002510"
     CDS             complement(14002..14463)
                     /gene="tsaE"
                     /locus_tag="OO327_002510"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR6019014.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14435..15982)
                     /gene="nnr"
                     /locus_tag="OO327_002511"
     CDS             complement(14435..15982)
                     /gene="nnr"
                     /locus_tag="OO327_002511"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR6019015.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15981..17120
                     /gene="queG"
                     /locus_tag="OO327_002512"
     CDS             15981..17120
                     /gene="queG"
                     /locus_tag="OO327_002512"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR6019016.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17103..17156)
                     /locus_tag="OO327_002513"
     CDS             complement(17103..17156)
                     /locus_tag="OO327_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019017.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3944 a         4958 c         4367 g         4117 t
ORIGIN      
        1 aaaccatcca aatctggatg gcttttcata attcagagga actagctgcg ctgacgaacc
       61 gcttcaaata agcaaattcc ggttgcaacc gaaacgttca gggaagaaac acttcctgcc
      121 attgggatgc tgatcaactc atcgcaatgt tcacgggtca ggcgacgcat accttcacct
      181 tccgcgccca tcaccagcgc caggcgtccg gtcattttgc tctgatagag tgtatgatcc
      241 gcctcgcctg ccgtaccgac gatccagata ttctcttcct gcaacatacg catggtgcgc
      301 gcaaggttag tcacccgaat cagcggaacg ctttctgccg cgccgcaggc tacttttttc
      361 gccgtagcgt tgagctgtgc ggagcgatct ttcggcacaa tcaccgcatg aacgcctgcg
      421 gcgtccgcac tacgcaggca cgcgccgagg ttgtgcggat cggttacgcc gtcgaggatc
      481 agcaggaacg gttgatcgag cgaagcgatc agatccggca gatcgttttc ctgatactga
      541 cgtcctggct tcacgcgggc gataatgccc tgatgcacgg caccgtcgct tttctcgtcg
      601 agatattggc ggtttgccaa ctggataacc acgccctgag actcaagggc gtgaatcagc
      661 ggtaacagac gtttatcttc acggcctttt aaaataaaga cttcctgaaa acgttcaggg
      721 gcgcgctcca gcagggcctg cactgcgtgg atgccgtaaa tcatttcgct cattaatgta
      781 ctcgttgttt acgttttgcc tgatgcacta cgtttatccg gcctacatga tctctgcaat
      841 atattgaatt tgcgtgatta tgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa
      901 caaagcgcat tttgtcagca atctaaccct cttcttttaa agagggtatt gatcactctg
      961 ccactttttt cttcgccgca cgcttcgctt tggtcgctgc ggctattttc tgcgttttcg
     1021 ccgatggctt tttcgctttt ctcgcgtctt tcttcgccgc tttcggcttc gtttttttct
     1081 caccgcggaa ggcgctgtct ggctcaaagt ttaccttttt accgacctga caacgcttgc
     1141 cgcctttttt gcctgcatcg ccttttttcg ctttctcgcg cgccgtttta ccgacgttgc
     1201 gcggtgcgcg ttcactggag atcagactaa agtcgatttt gcgctcgtcc atattaaccg
     1261 cttcgacgcg aacttccacg cgatcgccca ggcgataagt ctggccgctg gattccccca
     1321 tcaggcgttg ccctacctgg tcaaagcgat agtagtcatt gtccagcgaa gagacatgga
     1381 ccagaccatc aatgaacaag tcgtccagac ggacgaagaa gccaaagcca gtgacgctgg
     1441 aaattacgcc tttaaagacg ttacctacct ggtcgagcat gaagtcacac ttcagccagt
     1501 cagcgacatc gcgcgttgct tcgtcggcac gacgttccgc catcgaacag tgctgaccca
     1561 gttgcaacat ctcttccatc gaataatggt agccgccggt ttcagtggtg ttgccctgat
     1621 gcccctgctc tttcgccagc agatatttaa tggcgcggtg cagcgtcagg tcaggataac
     1681 gacgaatcgg cgaagtaaag tgcgcatagg actgcaatgc cagaccgaag tgaccacggt
     1741 tttctggatc gtaaatcgcc tgtttcatcg agcgtagcag catggtttgc agcatttctg
     1801 catcaggacg gtcggcaacc gactccagca gttccgcgta gtcacgcggt tccggcttgt
     1861 taccacccgg cagctccagc cccagctccg ccagcactga acggaaagag gtaatcgctt
     1921 cggtgctcgg cttgtcgtga atacggaaca gtgccggttc tttcgctttc tcaacgaaac
     1981 gcgccgccga gatattcgcc agaatcatgc actcttcaat taatttgtgc gcgtcgttac
     2041 gctgggtctg ttcgatacgt tcaatacggc gttcagcgtt gaaaatgaac ttcgcttctt
     2101 cgctctcaaa tgagatccca ccgcgttctt cacgggcttt atccagcact ttatagaggt
     2161 tatgcaactc ttcgagatgc ttaaccagcg gggcgtactg ctcgcgcaga tcctgatcgc
     2221 cctgcagaat atgccagact ttggtgtagg tcagacgcgc gtgagagctc atcaccgctt
     2281 cgtagaattt gtagcccgtc aggcggcctt tcgacgaaac cgtcatctcg cacaccatac
     2341 acaggcggtc tacctgcggg ttgagcgaac acaggccgtt agagagcact tccggcagca
     2401 tcgggataac ctgcgaaggg aagtacaccg acgtgccacg gttacgcgct tctctgtcca
     2461 gcggcgttgg cggacgcaca tagtagctga cgtcggcaat cgcgacccat aaacgccagc
     2521 cgccgccgcg ttttttctcg cagtaaactg catcgtcaaa gtcacgggcg tcttcgccat
     2581 caatggtgac cagcggtaaa tcgcgcaaat cgacacggcc cgcttttgct tcttccggca
     2641 cttcttcttt cagccccgca acctgttgct caacagcctg cggccagata tacggaattt
     2701 catgggtacg cagagcgata tcaaccgcca tgccggtgcc catattgtcg cccagcactt
     2761 cgacgatttt acccaccgct ttggtgcggc gagtcggacg ctgagtcagt tcgactacga
     2821 ccacaaagcc catccgcgcg cccatgatct gatcgggcgg gattaagata tcgaagctca
     2881 gacggctatc gtcaggaacc acaaagccga cgcccgcttc ggtaaagtag cgaccaacaa
     2941 tctggctggt ttttggcacc agtacgcgga caatacgcgc ttcacgacga cctttacggt
     3001 cagcacccag cggctgcgcc agcacctgat cgccatgaat gcaggttttc atctgctcgc
     3061 tggagagata caaatcatct ttacgccctt caacccgcag aaagccgtag ccatcacggt
     3121 gaccaataac ggtacctttc accaggtcga ggcgttccgg cagcgcatag cactgacgac
     3181 gagtgaagac cagttgacca tcgcgctcca tcgcgcgcag gcggcgacgc aggccttcaa
     3241 gctgctcttc gccttcaatg tgcagttcta ccgccagctc atcacggctg gccggttttt
     3301 cacgtttggt taaatgttcg aggataaatt cccgactagg gatgggattc gcgtattttt
     3361 cagcttcgcg ttcctggaaa ggatcttgtg acatctcggt tcctccgttg tcatctctga
     3421 tgaagatttt cgtcactcca ccagcaataa tttataaagc ggttgattct cttcaaccaa
     3481 atcggcaagc gtgtagttat ccagttccgt aagaaaactt tgcacggcct tagaaagcgc
     3541 ctgtttcaac cgacaggcag gtgtaatgtg gcaaaactca ctgctgcaat tcaccagcga
     3601 taagggctcc agctcgcgca ccacatcacc aatacgtatc gcactcgccg gtttacccag
     3661 gcgaatgccg ccattttttc cacgaacagc agtcacgtag ccggcacgac taagttgatt
     3721 gattattttg accatatgat tacgggagac gccgtagacg tcagtcactt cagaaatact
     3781 ggtcatccgc ccttctggca atgacgccat gtagatcagc gcacgtaatc cgtaatcagt
     3841 gaaactcgtt aactgcacat caacctcaaa agggaaatcg ggaaaaaata catttatatt
     3901 gatgataaac cagccacaag ctgtgtcgct aatttatttc agttcgggga aggaaaaaag
     3961 cgaggattta acactgtgtc ggatagcgta atggcttaac cgacacagca atgtatcaca
     4021 ctgttagccc ggcaggcaaa atatctgcca ggcgtaccag aattacgcgt cgaacgggtc
     4081 gcgcagaatc atggtttcag tacgatccgg accggtagag atgatatcga tcggcacacc
     4141 ggtcagctct tcaatacgct tgatgtagtt cagcgccgcc tgcggcaggc cgctacgatc
     4201 tttcacgccg aaagtggatt cagaccagcc cggcatggtt tcgtaaatcg gctctacacc
     4261 tttccagtcg tcagctgcca gcggagtggt agtcacttcg cgaccatccg gcatacggta
     4321 agccacgcag agtttaacct ctttcaggcc atccagaacg tccagtttag tcaggcagaa
     4381 gccagacagg gagttcagct gtaccgcacg acgaacggca acggtgtcca gccagccggt
     4441 acgacgacga cgacccgtag ttgcaccgaa ttcgttaccc tgcttgcaga ggaactcgcc
     4501 agtttcatca aacagttcgg ccgggaaagg acctgcacct acacgagttg agtaagcttt
     4561 gaggataccc agaacgtaat caacataacg cgggcccagg ccggaaccgg tcgccacgcc
     4621 accagcagtg gtgttggaag aagttacgta cggataagta ccgtggtcga tatccagcag
     4681 cgtaccctgc gcaccttcga acatgacgaa atcgccacgc tgacgcgcct ggtcgagcag
     4741 gtcagaaacg tcaaccacca tagaagtcag gatgtcggca acagccatcg tatcatccag
     4801 aactttctgg taatcaaccg cttcagcttt gtagtagtta accaactgga agttgtgata
     4861 ttccatcact tctttcagtt tttcagcgaa ggtttctttg tcgaaaaggt cgccaacacg
     4921 cagaccgcga cgtgccactt tatcttcata agcaggcccg ataccacgac cggtggtgcc
     4981 gatcgctttc gcgccacgcg ctttctcacg cgcgttatcc agagcaacgt gataatcaag
     5041 gatcagcgga catgcttcag acagcagcag acgctcacga acggggatgc cacggtcttc
     5101 cagttctttc atctctttca tcagcgcggc cggagacagc acaacaccgt taccgatgat
     5161 gctggttaca ttctcgcgga gaatacctga tggaataaga tggagaacgg ttttttcacc
     5221 gttgattacg agagtatggc ctgcgttgtg accgccctgg tagcgtacaa catatttagc
     5281 ccgttcagtc agaagatcga cgatcttacc tttaccttcg tcaccccatt gggtgcccag
     5341 tacgacgacg ttgttaccca tttttcaaaa tcaccgtttg cttaaaaatg gattctacca
     5401 tcgctttttc agagttacag cactttttgc actcaaaaat gacctcgatc agtctgtttt
     5461 ttgttcagcc aatcgttttc ctcaacatgt agtagaccac aacgcccgca accacaagtc
     5521 caccgccaaa acgacgtaaa atattatcgg gcaaattggt catcgcagag atcatcttct
     5581 tccatgcctt cgggtaaagc atcggcccta aaccttccag taccaaaacc agggcaagcg
     5641 ccagccagat tgtcgaattc attcattatc cttatagaaa aagaaaacca ccgacatccc
     5701 tgaggatgcg gtggctttat tgacctgtac cgcagtcgtt atattaacgc gttgcggaag
     5761 tcggcgtctt catgtagcgg aagaaatcgc tatccgggct catgaccatc acgtcctgat
     5821 tgccagagaa gctgttctca taagcacgca ggctacggat gaatgcgtag aagtccggat
     5881 ctttactgaa tgcatcagca aacagtttgg ctgcttcggc atcgccttca ccacgcataa
     5941 tgcggccctg acgctcagct tctgccagcg ttctggtcac ttcatagtcg gcagtagcgc
     6001 gcagtttttc cgcttcttcc tgaccttgtg aacggtgacg acgcgctacc gcttcacgct
     6061 cggcgcgcat acggttgtag atcgcttcag acacttcggt cggcaggttg atctgcttga
     6121 tacgcacatc gacaacttca atacccagcg ccgccatact gttcgggttg atgaccggaa
     6181 ctttgccctt cgtctcagcc gttacgcgct ctgccgcttc ggcaatggcg ttatctgctg
     6241 ccggggtagt aacttcatct tctgtacccg cagaaccgga gttcagcgcg tcacgtactt
     6301 cgagggtcag acgaccacgg gaatcggtga cgatatcttt cacgtccagg cgaccaattt
     6361 cagaacgcag acggtcagag aacttacgtt tcaacagcac ttccgcttgc gaaatgtcgc
     6421 caccacccgt tgccaggtag taacggctga aatcgctgat gcgccatttg atgtaagagt
     6481 cgacgatcag gtctttcttc tctttggtca caaagcggtc ggcctggttg tccatggtct
     6541 gaatacgtgc gtcgagcatt ttcaccgttt caatgaacgg tatcttgaaa tgcagaccag
     6601 gctcataaac caaaggtttg ttgtcatcgt cacgcagtac cttaccaaaa cgcagcgtaa
     6661 taccgcgctc accttctttg acgacaaaga cagacatgta aagcactacc agcacgatga
     6721 tgataatcgc gataactgac ttacgcatcg ttattccccc tgacgctggt agtcgttacg
     6781 ctgcgcgttg gcgcggcgtt ggtccataat atcgccctga ctggtggacg acgtgttgct
     6841 tgctccactg gttgtggagg aagaggctgg cggcagacgc agcagattgc tggcaccgtt
     6901 atcgctcttc gccgcagggg cgttaccacc tttcagcatc tggtctaacg gcagaaccat
     6961 caggttgcca cctttatcgt taaccagcac tttgcgggtg ttacccaaca ctttttccat
     7021 cgtctcgata tacagacgct cgcgagtaat ttccggcgcg gctttatatt ccggcagaag
     7081 tttagcaaag cgcgccactt caccctgagc ttccaggatg gtctgggcct tgtacgcacg
     7141 cgcctcttcg aggatacgtt gcgcctgacc gttcgcacgc ggctgaactt cgttggaata
     7201 cgcttctgct tcacgaatgt attgctgttc gttttcacgc gcggcaatcg catcgtcaaa
     7261 cgccgctttt acttcttccg gcggacgagc agcctggaag ttgacgtcca gcagcgtgat
     7321 acccatgtca tacggacgaa tcgtctcttc cagttcgcgc tgagtatcgc tacgaatcac
     7381 ggtacgacct tccgtcagaa tgcggtccat ggtgtattta ccgataactc cacgcagggc
     7441 gctgtcggta gcctgacgca ggctgtcatc cgggctggtc acgctataca gatatttttc
     7501 cggattggtg acgcggtact gcacgttcat ctcaacgcgc actacgttct cgtccgacgt
     7561 cagcatcaca ccagaagcgg ccagttcacg cacggcttcc acgttcaccg gtttgacttc
     7621 gtcgataaac gtcggtttcc agttcagacc cggctcaacc agatggctga atttaccaaa
     7681 gcgtgttacc acgccgcgtt cggcttcttt aatggtatag aaaccactgg ccgcccagat
     7741 aatgacaatc gctgccgctg cgatggtaac gacacgaccg ccaagctgcg ggcgcgggcc
     7801 ttgcgatgaa ctgccaccgc cagatccggt gcctttaccg ccgcccagac caccgagctt
     7861 tttgctcagt ttgcggaaga tatcatctaa atcaggtggc ccttgatcgc gaccgccttt
     7921 gtttccattt ccctcagagt tgccgccagg tttgctgctt ccccacgggt cgcggtcttg
     7981 tccgttatta ccgggctgat tccacgccat gtttgtgctc catatttgtt atgcggtgat
     8041 ccctgttgtc tttcgacgcc agaggatatg actccacgct tcagacgcta cgccgttaga
     8101 tcaggtaatc gatcaacgcc ggttcttgtt tacagaggcg acgccagtca acgatcggca
     8161 tacgaacttg cagacttacg ctgccgtcct cctccatcca ctctttttct attgcctgaa
     8221 gctgataaaa acgactcctc agacgccctt cctgcggtgg cagacgcaat gtatgctgcg
     8281 ccacctcgcc ggaaagccgc tccgtcaaag cctgaaaaag ctgtggtatc cccgctccgg
     8341 tctgtgcgga aagccagaca cggatcggtt tgttctcttc gtcccgatca atacgcggtt
     8401 cgaaatcttc cagcatatcg atcttgttca tcaccagcag ggttgggatc tcgtgagcgt
     8461 cgatctcttc aagaaccgta ttcaccgctt cgatgttttc ttgtacacgc acatccgccg
     8521 catcaatgac gtgcagcagt aatgtggctt gccgcgtctc ttgtaacgtg gctttaaatg
     8581 ccgccaccag atcgtgcggc aggtggcgaa taaaccctac ggtatctgca agtacggttt
     8641 caccgacatc tgcaacgtca atacgccgca acgtcgggtc gagggtggca aacaactggt
     8701 ctgccgcgta gacccgcgct tcggtgatgc gattgaaaag ggtagattta ccggcgttgg
     8761 tatatcccac cagcgaaaca gtaggaacgt cggctttgat acgcgattgc cgcccctgct
     8821 cacgctgctt ttcaactctt tccaggcgcg actgtatctg cacgatgcga ttacgcaaca
     8881 aacgacggtc ggtttcgagc tgggtttcac ccggaccacg caaacctatc ccgcctttct
     8941 gtctttcaag gtgggtccag ccacgcacca ggcgcgtagc cagatggcgc agctgcgcca
     9001 gctcaacctg caacttaccc tcatgggtac gcgcacgttg ggcgaaaata tctaaaataa
     9061 ggccggtgcg gtcgataaca cgacactcgc acaaacgctc caggttacgc tcttgcgccg
     9121 ggctcagggc atggtcaaaa agaacgaccg aagcacccat cgctttgaca gcttccgcaa
     9181 tttcaactgc tttaccttca cctacaaaat actttgggtg cggcgcttta cggctaccgg
     9241 taatcacctg caatgcttcg acaccggcgg aagagaccag agattcaaac tcctggaggt
     9301 cttccatatc tttgtcttgc gtaaaataga tgtgtaccag taccgcctgc tcaccagcat
     9361 cataacggtc aaacaagcgt ataaccctct aaatagatca gcggggaacg caggatcgct
     9421 ggctccccgt gtaaaaaaac agcccgaaac cttattcggt ttcttcgctg tcctgttgcg
     9481 cggaagtatt ctgcgcgctg ctaccatgat ggtagttact gctggtaccg ccaccggcgt
     9541 tgttactgtg atgagaaacc gggcgagatg ggacaacagt agaaatcgcg tgcttgtaaa
     9601 ccatctggct gaccgtgttt ttcaacagga tcacgaactg atcaaaagac tcgatttgcc
     9661 cttgcagctt aataccattc accaaataaa tagaaactgg aacacgttcc cgacgcagtg
     9721 cgttcaggaa cggatcttgt aaagattgcc ccttagccat tctctctttt ccttatatgc
     9781 ttatttgtac tttgaacctt tcgattctga aaaaattgcg cacgatacgt ctcaattgta
     9841 cacattcagc ctgcgatagc accaacaacc tgtaatactt cgtcacgcgc ctgttctggt
     9901 ttttcactgt caagccagtg aaccccttcc caaccacgca gccaggttat ctgccgcttc
     9961 gccaactgtc tcgtggcgca aacacctcga taaaccattt catcgtatga gatttcgcct
    10021 tcaaggtaag accacatctg gcgataaccc acgcaacgaa tggaaggcaa gtccgtatgc
    10081 aaatctcctc gggcaaaaag cgcccggact tctgcttcaa aacctgaagc caacatctga
    10141 tgaaaacgct gctcaatgcg ttgatggagc agttcacggc tcgccggggc gatggcgaac
    10201 tgatgcacct gatacggtag agcgtctcct gacgtttgcg tcagttccgt taaagtttta
    10261 cccgaaatga aaaaaacttc cagtgcccgg gaaagccttt gtggatcatt tggatgaatc
    10321 cttgctgccg caaccggatc tacctcctga agttgacgat gcaatgactc ccaaccttgc
    10381 tctgccgcct gttgctcaat tctggcccgc acttccgggt ctgccgacgg tagcggcgac
    10441 aacccttcca gcaatgcctt gaaatacaac atcgtaccgc ccactaacag tgggatccgc
    10501 cccgccgcgg tgatatcggc catttccgcc agcgcatcgc ggcgaaaatc agcagccgag
    10561 taagcctgcg acggatcgcg aatatccagc aatcggtgcg gcgcggcgag taactcttca
    10621 gcgttcggct tcgccgtccc gatatccatc cctttgtaaa taaggacaga atcaacgctt
    10681 atcaactcta ctggtaaaat tttacgcagc tcaatggcta acgccgtttt accggaggcc
    10741 gtcggcccca tcaaaaaaat cgccttaggc aggctcgcct tactgatatc actcatcttt
    10801 cagggctttt atcgccggat gtaaatcaac agattgtaac agaccacccg gcggcgtttt
    10861 cacaagttgc ggacataacc gttccacgtc cgccagcagg gttatggcct gtgccattga
    10921 ccactgcgca tgttcgctca tcagatttcg tgcaatccac tgcgcaatat tgccaggttc
    10981 gaatacggac tgcttcgcca ggtagcctat cagttcagga atcaagattt gtaaattttg
    11041 ttggcgtaag ggtaaaggca cggccctgat ggtcacatgc tgtgcatctg actggaaatc
    11101 aatacccaat tccgctaggg cagactgcgc tttttctaat gccgattttt cttcgccaga
    11161 aacttttagc cgcaacggaa tcagcagcgg ctgggcgcaa acgggcgctt cacccggcgt
    11221 caattgtgcc tgacgcagcc aacgttctgc cactggcaag gataaaagtg aaatgttgcc
    11281 atcgcgctcc agcaacgcac agtcggaatg gacgatagtc agtacccgac caaaactctg
    11341 actgttcgcc gcaagtgcag gttcctgcgg ttccggcgct tttggttttt gcatcggcgc
    11401 gggcgtttgc aaaagctggc gatacacttc accttgctgt ttctggtagc ctggctgcgc
    11461 attcggccag ggggcagccg gacgactgcc tgaggcgggc gcaggagagt agcgcggagc
    11521 taccggctca cgagctgccg gttctgcaaa gtgattgcgc cccgccgcca cgcggttttc
    11581 cgggatggca cgcggtgcgg gttgtggttc atcgtccagc ggtagcggcg tttccagttg
    11641 ctgttgtagc acgctcagca cgccctgata gataaaatca tgcaccagac gcgactgatg
    11701 gaaacgcact tcgtgtttgg cggggtgtac gttgacgtcc acctgatgcg ggtcgatctc
    11761 cagatacaac acaaatgccg gttgctgatc ggcccccagt ttgtcttcgc aggcctggcg
    11821 gatcgcatga ttgatcaggc gatcgcgcat catgcgacca ttcacgtagc aatactgaat
    11881 ttccgccagt gcgggcgtag tgtgatttgg atcggccacc cagccgcgca gggtgagatc
    11941 gccgtgttgc cattcaatcg ccagcgcttg ttcgagaaaa gcggtgccgc aaatcgcgcc
    12001 taagcgccgt tctttttgcc cgccttccgg cactgcgcgg tactgacgca caattttacc
    12061 gttatgcgac aggttgatcg tgacgtcgaa acgcgccagc gcaatgcggc ggatgatttc
    12121 atcaatatgg ctaaattcgg ttttctcggt gcgcaggaat ttgcgccgcg ccggggtgtt
    12181 gtagaacaga tccagcacct ccagcgtcgt tcccaccgga tgcgctgccg gtttaaccgt
    12241 cacgtccatg tcgcgtcctt cggcataggc ctgccaggct tcctgctgtt ctgcggtgcg
    12301 tgaagtgagc gtcaggcggg aaaccgaact gatactcgcc agcgcctcac cgcgaaagcc
    12361 caggctgata atggcttcga gatcgtccag agaggcgatt ttactggtgg catgacgagc
    12421 cagcgccagc gccagctcat cttttttgat accgcagccg ttatcacgaa tgcggataag
    12481 tttcgcccca ccgcgttcga tatcaatatc gatacgcgtc gcacctgcat cgaggctgtt
    12541 ttccactagt tctttgacta ccgacgcagg tcgctcgacc acctcacctg cggcaatctg
    12601 gttcgccagt tgtggcggta agacctgaat tggcatcaat cgtccttagt ttggcagcgt
    12661 gcgatctggc gtcgtcaccg tactggcagt ttgtgccgtt gcaccctgcg gcgcagattg
    12721 catcggatgc gcaaggaagt aattgcgcag gcctttgtaa atggcttctg ccagctgttg
    12781 ttggtaatcg tcgctcgcca gcaaacgttc ttcgctgttg ttgctgataa aaccggtttc
    12841 gaccagtact gatgggatat ccggcgagcg cagaacgcca aggctggcgt gttctggtcg
    12901 acgtttatgt atttcgccaa tgcgttgcaa ctgactgatc atactggtcg ctacatcata
    12961 ccctacccgc tgggaatgac cgaactgtaa atccagcacc gcctggctta aataggggtc
    13021 agactgactg ttcgccagca catcacccgc cccacccagc agctccgaca gtttctcgtg
    13081 ctgctccagc cagctggcca tttcactgtt ggcgcgacgg ttagagagca cccatacgga
    13141 agcgccagtc gcactacggt tcggtgcggc atcagcgtga atcgacacga ggaaattggc
    13201 gttttgctta tgtgccacat cactgcgccc catcaccgag ataaagtaat ccccgtcacg
    13261 ggttaaaacg cctttaaaca tcggatcgtc attgagcaaa gtacgcaatt tacgcgcgat
    13321 ggcgatggtg acatttttct cccgcgtacc accggggccg atagcgccag ggtcctgacc
    13381 gccgtgtccg gcatcaatag cgatgataat tttatcgcca gtgttagccg tcgcgcgcgc
    13441 tgccggacgc gttaccgtat tactgctgat aacacccgta gtgcggttac tttccgtttt
    13501 aaacggattg cgcgccggtt cgctgacgcg cggtgcgaca accgcaggcg tttcaacgcg
    13561 tttcgcaacc acgggcggcg gaggaggcgg tggcggcaca tcggcgttaa tcgtaaagac
    13621 gacagtgtaa ttgctgccat tctgccgctt caccgcttcg gttttaccgt tttcggtaag
    13681 atcgaccacc agccgtagcg tttgtgcatc tttaggcgtt ccagagcgaa tcgccttcac
    13741 cagattattg ccgctgaaca acaacggcag tccctgaatc atgcccgttt gtttgatatc
    13801 gagcgccacg gtgcgtttgc tttgatggct aaacgcataa tcaggatcgc caataaaact
    13861 caacgttatc cgcgcctgtt gattaccgtt agaaacctga atatcagaga gcgtcgcggc
    13921 acccaccggc gtgcacagca gcagcagcgt cgctaccaac caatttctga tgcgatacat
    13981 catcccgcca cctttcaaag gttaaccggc taaacgcgcc agcaacaatt cacccgcaga
    14041 ggaaaccgca ctcacgcgcg cctcacggcc ttgtgcctga taatcaatgt gtatttcgac
    14101 atccgggtca ggaagaacac ctgtaccttg ttgtggccac tccaccaggc agatggcatc
    14161 gttggcaaaa taatcgcgga tccccataaa ctccagctcc tcgggatcgg caaggcggta
    14221 caaatcaaag tgatagacca ttaagttgtc gagcgtatag ggttcgacca gcgtataagt
    14281 ggggcttttg acattaccct gatgacccag agcctgtaaa aagccccggc taaaggtggt
    14341 tttacctgcg cctaaatcgc catacagata gattacggtt gcgccatcgc aggctttcgc
    14401 tacccgctcg cccaggtcta atgttgcctg ctcatcaggg agcggaatta ctcgattcat
    14461 catggttttt atcagtcact tccgggttaa caatacgctg tagcgtggaa aagagatcgg
    14521 ttgccagcat cccgcgcgtt ccaaaacgcg ccgccagtac gtcagctgcc gcaccgtgcg
    14581 cgacacagcc tgcacaggct gcatcatacg gcgacagttt ttgcccaagc aatgcgccaa
    14641 taataccaga gagcacatcg cccatgccgc cgctcgccat gcctgcattt ccggcatcaa
    14701 taatgcctaa agcgtcagga tgggcggcga ccacggttcc ggcacctttc agcaccgcta
    14761 cgccgccata acgttgtacc agacgtttgg cgcaatgtaa gcggtcactt tcaatttcag
    14821 cgacggaaca gcctaacaac cgtgcggcct cgctaggatg cggcgtgatc acgcgattgt
    14881 gacgcttatc gggattgatt gccagcaggt tcaatgcatc ggcatcccac aacatcggtt
    14941 tgcgaaaatt ctcaactttt tgcagggctt ttttccccca ctcttgctgg cccagaccgg
    15001 gaccaatcac caccacatcg gcccattcca ggctttcggt aagagagtcc atagtcagtt
    15061 catgcaccat caattctggt cgtgcagtca gcagcggcgc aatgttctca ctgcgggtca
    15121 gtactcggac taaaccagca ccagcacgta gcgccgcttc ccccgtcata cgaatagccc
    15181 ccgccgtgcc gtgatcgcca ccgataatca ccagccgccc gtgatcgcct ttatgcgaag
    15241 tcgggcgacg cggtttcagc cagtgagaaa gttgttctgc cgaaaaccgc tgaattttcg
    15301 tctcctgacc tgccagccag ctatccagcc ccagtgagtc aaaatgcagt tgtcctgtaa
    15361 catcccgcgc ttttccagtg agcaagccta gtttcagcgc aataaaagtg atggtgtgat
    15421 cggcgttgat cactgcgcct ggcgtagcgc cggtttcagc cagcaggccg gaagggatat
    15481 caaccgccac aatcggcgca ggatgggaat tagcgtggtc gattaactgg ctaatggatt
    15541 cgcggggcgc ttgctgcaag ccggtaccga gcagcgcatc aacaatcaga tctaccgatt
    15601 cgggccagac aatattcgaa gcatggatct cgccacccgc gtttaaccat gcttcgcgtg
    15661 ccagcgcggc ctcttccggc aacggtttgt cgctctcctg ggccaacaac gtgacctcaa
    15721 tgccgaccgc tttggccagt cgcgcgacca cgtagccatc gccgccgtta ttaccatgac
    15781 cgcacagcac cagccagtgg cgggcgtcag gatacgccga acgacacacc tggaatgcgg
    15841 cctcgccagc gcgaagcatc agctcataga gtgtgagccc cagcacatct gccgcctcgc
    15901 gttctccgcg gcggatatcg tcggcgtacc agacggtgtg tggtatactt acggggtttt
    15961 tcttcattgt atggtccgtc atgtcagagc ccctcgatct caatcagtta gcgcaaaaaa
    16021 ttaaacagtg ggggctggaa ctgggctttc agcaggtagg tattaccgat accgatctca
    16081 gcgagtccga gcccaaactg caagcatggc tggacaaaca ataccacggc gaaatggact
    16141 ggatggcacg tcacggtatg ctgcgcgctc gcccccatga gttattaccc ggtacgctgc
    16201 gtgtaatcag cgtgcggatg aattaccttc ctgctaacgc cgcatttgcc agcacgctga
    16261 aaaaccccaa actcggctat gttagccgtt atgcgctggg ccgtgactat cacaaacttc
    16321 tgcgcaaccg actcaaaaag ctgggcgaga tgattcagca acattgtgtt tcgctgaatt
    16381 ttagaccgtt tgtcgattct gcgcctattc tcgagcgccc gttagctgaa aaagctgggc
    16441 tcggctggac aggtaagcac tcacttatcc tcaatcgcga ggccggttcg ttcttctttt
    16501 taggcgaatt gctggtcgat attccgctgc ccgtggatca accagtcgag gaaggatgcg
    16561 gcaaatgcgt ggcctgtatg acgatttgcc cgaccggtgc catcgtcgag ccatataccg
    16621 tcgatgctcg ccgctgtatc tcttatctca ccatcgaact tgaaggggcg ctcccggaag
    16681 agttgcgacc gttaatggga aaccgtattt acggttgcga tgactgccag cttatctgcc
    16741 cgtggaatcg ctattcgcaa ctcactacag aagacgattt cagcccgcgt aagccgctac
    16801 acgcaccgga actcattgag ttatttgcct ggagcgaaga gaagttttta aaagtcacgg
    16861 aaggatcggc gattcgccgt ataggtcacc tgcgctggct gcgtaatatc gccgtggcat
    16921 taggcaatgc cccttgggat gaaacgattt tgacagcgct ggaaagtcgt aaaggtgagc
    16981 acccacttct cgatgagcac atagcgtggg cgattgcgca gcaaatagag agacgaaatg
    17041 cgtgcatagt cgaagtgcaa ttgccgaaaa aacagcgtct ggttcgggtg attgaaaaag
    17101 ggttaccgcg tgacgcctga ttcatccaca gcttgtgaat aaaaataaaa acgcattgca
    17161 attcaagacg aataaaatcg tcaagtgatc gactcgacaa aacggaatct tgatttttaa
    17221 cttatttatc aataggttaa aaaaatacca ttcaccgtgc gaagtttctt tgaaattaag
    17281 attacagcct gaggctgtgg ataactctgt ttacaagagt ttgtcagaga caacaaaaac
    17341 cagcaataac gcatcgcgtc gctgtagata ttttattgag agaaga
//
LOCUS       DAJTSI010000084        17265 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000084 DAJTSI010000000
VERSION     DAJTSI010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17265
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.084"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            445..1011
                     /gene="mntP"
                     /locus_tag="OO327_002514"
     CDS             445..1011
                     /gene="mntP"
                     /locus_tag="OO327_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR6019018.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(1008..1817)
                     /gene="rlmA"
                     /locus_tag="OO327_002515"
     CDS             complement(1008..1817)
                     /gene="rlmA"
                     /locus_tag="OO327_002515"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR6019019.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1983..2192)
                     /gene="cspE"
                     /locus_tag="OO327_002516"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1983..2192)
                     /gene="cspE"
                     /locus_tag="OO327_002516"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6019020.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(2205..2348)
                     /locus_tag="OO327_002517"
     CDS             complement(2205..2348)
                     /locus_tag="OO327_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR6019021.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(3019..3306)
                     /locus_tag="OO327_002518"
     CDS             complement(3019..3306)
                     /locus_tag="OO327_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR6019022.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            complement(3381..3524)
                     /gene="mgrB"
                     /locus_tag="OO327_002519"
     CDS             complement(3381..3524)
                     /gene="mgrB"
                     /locus_tag="OO327_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR6019023.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            3683..3922
                     /locus_tag="OO327_002520"
     CDS             3683..3922
                     /locus_tag="OO327_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019024.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            complement(4065..4856)
                     /gene="kdgR"
                     /locus_tag="OO327_002521"
     CDS             complement(4065..4856)
                     /gene="kdgR"
                     /locus_tag="OO327_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR6019025.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            5033..6406
                     /locus_tag="OO327_002522"
     CDS             5033..6406
                     /locus_tag="OO327_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6019026.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            complement(6452..7333)
                     /gene="htpX"
                     /locus_tag="OO327_002523"
     CDS             complement(6452..7333)
                     /gene="htpX"
                     /locus_tag="OO327_002523"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR6019027.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(7525..9573)
                     /gene="prc"
                     /locus_tag="OO327_002524"
     CDS             complement(7525..9573)
                     /gene="prc"
                     /locus_tag="OO327_002524"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR6019028.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(9593..10291)
                     /gene="proQ"
                     /locus_tag="OO327_002525"
     CDS             complement(9593..10291)
                     /gene="proQ"
                     /locus_tag="OO327_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR6019029.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(10388..10885)
                     /gene="msrC"
                     /locus_tag="OO327_002526"
     CDS             complement(10388..10885)
                     /gene="msrC"
                     /locus_tag="OO327_002526"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR6019030.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            11015..12298
                     /gene="yebS"
                     /locus_tag="OO327_002527"
     CDS             11015..12298
                     /gene="yebS"
                     /locus_tag="OO327_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCR6019031.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            12267..14887
                     /gene="yebT"
                     /locus_tag="OO327_002528"
                     /pseudo
     CDS             12267..14887
                     /gene="yebT"
                     /locus_tag="OO327_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            14967..16406
                     /gene="rsmF"
                     /locus_tag="OO327_002529"
                     /pseudo
     CDS             14967..16406
                     /gene="rsmF"
                     /locus_tag="OO327_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            16524..16760
                     /locus_tag="OO327_002530"
     CDS             16524..16760
                     /locus_tag="OO327_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR6019032.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            16865..17056
                     /locus_tag="OO327_002531"
     CDS             16865..17056
                     /locus_tag="OO327_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCR6019033.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGSCF
                     PIEDFIDGFWQPAQEYGDF"
     gene            complement(17081..>17265)
                     /locus_tag="OO327_002532"
     CDS             complement(17081..>17265)
                     /locus_tag="OO327_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HCR6019034.1"
                     /translation="EWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHRV
                     DIGNLHYIDTGAVFGG"
BASE COUNT         4187 a         4354 c         4161 g         4563 t
ORIGIN      
        1 gtatcaactc aataacttat gaatttagcc aaagctatgt ttagtgtatt tttaataatc
       61 agacatagct taggctatat tatctcttcc cttatttgtt atttatttta acgtttcatt
      121 gatatataaa cataaatgaa aatcgttatc aataaagcaa tgaaataata tattccaaca
      181 gttgttttat attctcaaaa tatgttaagg ttgcgccctc atttggggag tagccgattt
      241 ccagattccg gaaatgtacg tgtcaacata ctcgttgcaa aacgtggcac gtacggactg
      301 aagactttca gtcaggcgag accatatgca catcaatcgc tatgcctgca tgaacgcgaa
      361 aacccatcgc ggcttgttgt tcatgtatgc atgtttgctg ggggcgatga tgtgttttat
      421 ggataccccg gtcaggacgt tgtcatgaat atcactgcta ctgttcttct tgcgtttggt
      481 atgtcgatgg atgcatttgc tgcatcaatc ggtaaaggtg cccccctcca taaaccgaaa
      541 ttttctgaag cattgcgaac cggccttatt tttggtgccg tcgaaaccct gacgccgctg
      601 atcggctggg gaatgggcat gttagccagc cggtttgtcc ttgaatggaa tcactggatt
      661 gcgtttgtgc tgctgatatt cctcggcggg cgaatgatta ttgagggttt tcgtggcgca
      721 gatgatgaag atgaagagcc gcgccgtcgg cacggtttct ggctactggt aaccaccgcg
      781 attgccacca gcctggatgc catggctgtg ggtgttggtc ttgctttcct tcaggtcaac
      841 attatcgcga ccgcattggc cattggttgt gcaaccttga ttatgtcgac attagggatg
      901 atggttggtc gctttatcgg ctcaattatt gggaaaaaag cggaaattct cggcgggctg
      961 gtgctgatcg gcatcggcgt ccagatcctc tggacgcact tccacggtta ataagaacgc
     1021 tgccagaggt gaatattaaa gtccgtctgg cagtcgaaca cttcttttgc tgccagtgtt
     1081 tgccagactt ctggcttcgc acgccaggca aacggcgtca tctgcaataa tgcgacggct
     1141 tcatcaccgc gaagacgcat cggataacac aactccgcac tctgctgtaa tgtaaaacct
     1201 tccagttgtt ctgcatgagg tgcatgaaga tgtacttcat tgtaaatcag ccccttcagc
     1261 tccatcaaat gtcgcggtcc cggcgtggca gtaatgaccc agccgccggg cttcactact
     1321 cgtgctaatt cttctgcttt acacggcgcg taaatacgta ttatggcgtc catactggta
     1381 tcggaaaacg gcaaacggtg gctggaagcg acacaaagag tgacctgcgg atagcgtttc
     1441 gccgccgctt ttatcgctac cttcgagaca tccaggccaa acgtggtgat ttcgggcaac
     1501 gcatcggcaa atgcgtgtgt gtaataccct tcaccacagc caatatccag caccgccgtg
     1561 gccttttcat caagccgttc ccgcagttgg gcgacaattg catcacgcag cggctgataa
     1621 tgtccggcat ctaagaatgc gcggcgtgct tgcatcattt ccgcgctgtc gcccggatca
     1681 cgagaccgtt tatgctgaac gggcagcaga ttgacatacc cttctttcgc catatcaaac
     1741 tgatgtcgtt ggggacagat atagctgttt ttttcacgcg aaagaggctg atggcaaagt
     1801 ggacaggaaa acgacatgac aactccggca ggatactaaa ggccgcaagt gtaacgcgaa
     1861 tcacgcggcg ggcaaatatc cgaacgccag gagcgaagat aaagcatagt aaaaagcctc
     1921 gcattcgacg aggctttata gcgagattga agcgtattca cacttcagat cagtggattc
     1981 gatcagatag ctgttacgtt aacagctgcc ggacctttct ggccgtcctg aatttcgaac
     2041 tcaacgttct gaccttcagc cagagttttg aagccattac cctggatagc ggagaagtgt
     2101 acgaacacat ctttgctgcc atcagccgga gtaatgaagc caaaaccttt agactcgttg
     2161 aaccacttaa cctgaccttt aatctttgcc atttgaaaaa ttccttagat tgttttcttc
     2221 gcccgcaggc ataacataga taaaactgac acattactgc atgaggcacc aatataaggc
     2281 tcggcagaga agcggtattc aacgtcaacg tgtttactca ggacttcttt actgaaaatg
     2341 ccacacataa acagaactgt acctcgttta acccgaaatc ttttatcaca tacaacgtta
     2401 attatggcaa gccattttta aacatatctc gatcagacgc acaaatctcg atacgtcttc
     2461 cacttttttt gcacacttat gcaacggaat acgcgccgat atatcattgt gcttaacctt
     2521 gccagttcag gcagatactt aaactggcgt agtttctaac atagttcaat aagttcagtt
     2581 gttccaggcc agagggagaa gttattacat agtgcgtgca atatcacatt ttctgctatg
     2641 caatgaataa aaagttatat cactttttct cataaaacag tcagttaacg gctatcaatt
     2701 accctaaaga gaagtcaatc cccaaaggga ttgtaaattt aaaataagaa aaattgatga
     2761 gtgagcaaaa aaaatcaaga gagaaacgtt tcgctaataa ataataccac gcttaatcac
     2821 aaatctgatg taaacatcct aataaattaa tgaggaaaat atctttttct gatatcccgt
     2881 tacgcaaata aataatatga taattatttg aagtcagacc agagttttaa tttgaagcag
     2941 ttgcaccatg acagtgcgta taatcaggat gaattgagta acccttcaac gacggggtta
     3001 ctcaagattg aggaaggatt atcgttcggc gaccaggcga ataatatctt cgtcatcaac
     3061 cgatttttga ctctcaatgg tctttttatc tgctttctct ggttcgtttg cttcgcacaa
     3121 acgacgtaac agtgcatttt gacgtttttg ctgatccagc aacgcctcaa gcagttcaat
     3181 ctgttcgtta gtccgtgaac tggcacgatt gataaaaaac cacaagatga gcccgataag
     3241 aagaaccacc accgatacaa ccaaagacgc aatgttcatc acgcctgaat ttacaacttc
     3301 gttcatttca ccacctctga gtagaggcgc tattctacca ctgctggaga ggaagaaaat
     3361 ctagtgctga aaaaatgata tcaccacggg ataaactggt taatggcaca aattccgctg
     3421 aaaaattgta catcctgatc gcacatcatg ttgaatacct gcgcccaaag cagcaagcaa
     3481 gccaacacca cgacaaccag aacgacccat cgaaactttt tcactccaat ctccgcttca
     3541 cctaccttat gtcattaaac tagcatgcgt tagttaaaca gcgcctaact atgtcgatat
     3601 tcgtgctttt tcggaatgag tagcttgctt cagcgcatat tgccgctacg attaagcgaa
     3661 cataaaaaga gaagagattg taatgcgatt catcattcgc acagttatgc tgatagcact
     3721 ggtatggata ggattattac tcagcggata tggcgttttg attggaagca aagagaatgc
     3781 cgcaggatta ggtttgcaat gtacctatct gactgccaga ggcaccagca cggtacaata
     3841 cttgcacact aaaagtggtt tcttggagat aacagattgc ccactgttac gtaaaagcaa
     3901 tatcgtggtc gataatggtt aagggtaata aataaccaga ttctcactca tgagtaaaat
     3961 attcttatag caagcgcgtt taatataacg caccttcaac tttatttttc tcatgctaaa
     4021 taaagaacct gtctgtttga tattccgaca ggttcttact ctcatcagaa cggatagtcg
     4081 tgataaccca tttgggcaga aattttgcgc gctgcggtat gcaacattgc gacatattcc
     4141 tgtaaacgct cttcagagaa acgcaacgtc gggaaggaga tgctcaaacc ggcaatgacc
     4201 acgccaaagc gatcaaatac cggtaccgca atgcattgca gcccttcttc ctgctcttcg
     4261 ttatcttctc catacccctg ctcgcgcacc tggtccagaa cgggtaataa cgcttctgta
     4321 ctggtgatgg tccgctcggt actgcgttta tactccacgc cctcaagaat ttgcttcact
     4381 tcatcgcgat cgcgccatgc cagcagtacc ttaccaatcg cggtgctgta cagcggatta
     4441 cgacgcccaa tccgtgaata catgcgcaaa ttgtacatag agtcaatttt gtgaatgtaa
     4501 acaatactgt cttcgtccag tgcgccgagg tggatagttt ctttggtcag gcgggagatc
     4561 tcacgcatct ggatatctgc gctacgaatt aaatcgacgt tttgtaacgc gcgagcgccc
     4621 agttcaaaca atttcagggt cagggaatat ttctccgact ccccttcctg cgccacataa
     4681 cctaaggttt tcatggtctg taaaaagcga taaacggtgc tttttgacat catgacgcgc
     4741 tgcgacagct cggttatccc tatttcgcgc tcttcaccca gcgcctgcaa aatgccaaaa
     4801 acttttagca cggaagatac agaatcaggc tgtttatcca gatctgcgtt agccatttat
     4861 cacctcattg cgagtgtttt ataaaaatca gaactgtttt ttattataat ttcgcaccag
     4921 ggtggtcgca atccatcttt tgccggttag ttacaattct gcgacatcca ccgtgaatat
     4981 cagtgctaga atcatacccc tgttgattat tcaccaaaga tataaaattc ctatgccaaa
     5041 agttcaggcc gacggcctgc cattgcccca gcgatacggt gcgatattaa ccattgtgat
     5101 tggtatttcg atggctgtcc ttgacggcgc aatcgccaac gtcgccctgc caacaattgc
     5161 cacggacctt catgccacac cagccagttc catctgggta gtgaacgcct atcaaatcgc
     5221 cattgtcatc tccctgctct cgttttcgtt tctgggcgat atgtttggct atcgacgtat
     5281 ttataaatgc ggtctggtcg tttttctgtt gtcttcactg ttctgcgccc tttctgattc
     5341 gctgcaaatg ctcacccttg cgcgtgtcat acaaggtttc ggcggtgcag cgttgatgag
     5401 cgttaatacc gcacttatcc gcctgatcta tccacaacgt tttctgggga gagggatggg
     5461 cataaactcg tttattgttg ccgtctcttc tgctgccggg ccgacaattg ctgcagcaat
     5521 cctctccatc gcatcctgga aatggttatt tttaatcaac gtaccgttag gtattatcgc
     5581 cctgcttctg gcgatgcgtt ttctgccacc caatggttct cgcgccagta aaccccgttt
     5641 cgacctgccc agcgccgtga tgaacgcgtt aaccttcggc ctgcttatca ctgcgttgag
     5701 tggtttcgct caggggcaat cgctgacgtt aattgctgcg gaactggtgg taatggttgt
     5761 tgttggtatt ttctttattc gccgccagct ttctcttccc gtaccgctgc taccggtgga
     5821 tttactgcgt atcccgctgt tttcactttc tatttgcaca tctgtttgct ctttctgcgc
     5881 acaaatgctg gcaatggttt ccctgccctt ttacctgcaa accgtgctcg ggcgtagtga
     5941 agtcgaaaca ggtttacttc tgacaccgtg gccgttagca acgatggtga tggctcctct
     6001 ggcaggctat ttgattgaac gcgtacatgc aggattgctg ggtgctttag ggttgttcat
     6061 catggctacg gggctttttt ccctggttct gctgcccgcg tcacctgcgg atatcaatat
     6121 tatctggccg atgatcttat gtggcgctgg atttggctta ttccagtcac ccaataacca
     6181 caccattatt acctccgctc cgcgcgaacg tagcggtggg gccagtggca tgttaggaac
     6241 ggctcgtcta ctgggtcaga gtagtggcgc ggcgctggtg gcgctgatgc taaatcagtt
     6301 tggtgataat ggtacgcacg tctcgctgat ggctgcggct attctggcag tgattgctgc
     6361 ctgtgtcagt ggtttacgta tcactcagcc acgagccagg gcataaaaaa agcgcgtcga
     6421 tcaggacgcg ctttttagta tttacttcat attacttcag gtattcaccc gtacgcagag
     6481 cttcaatacg tttatccagc ggcgggtgag tcatgaacaa ctcactgagc gatttcgact
     6541 taccgttaat gcagaaagcc atcatgctgg ttgcttcttg cggttcatag ctggttttca
     6601 ggcgctgcag cgcggcaatc attttctcgc gaccaaccag ttttgccgaa ccggcatctg
     6661 catggaattc acgatgacgc gagaaccaca tggtgataat gctcgccaga ataccaaaca
     6721 ccagttccag aaccgttgca accgcaaagt agatcagcgg gttgccgttg ctctcttcac
     6781 cttcatcacg atttccgccc ataaaacccg cggcaagctg cgccagaata cgggaaataa
     6841 agataacgaa ggtgttcacc acgccctgaa tcagcgtcat ggtgaccata tcaccattgg
     6901 cgatgtggct gatttcgtga gcaattaccg cctcggcttc atccgggctc atgttctgca
     6961 gcaaaccggt gctgacagca accagagagg catcacggcg cgcaccggtt gcaaaagcgt
     7021 tgatgtccgg cgcatggtag atagccactt gcggcatagc gatccccgcc tgacgagcct
     7081 gggttgctac agtattgacc agccaacgtt ccctttcgtt acgcggttgc tcgatcactt
     7141 ccccgccaac agatcgtaat gccatccatt tggacatcag aagcgaaacg aaggaaccac
     7201 caaaaccgaa cagcaaggcc atgatcatca gcccctgaac gctgctcgac tgtatccctg
     7261 tcaggctcag taccagcccg aaaacgacca ttacggccag gttcgttagc aggaagagcg
     7321 cgattcgcat cataattttc tttttacctc agtttaacaa aacgcaatat gcgataccca
     7381 catcgtatgg gttacgcgac tattttcaag tctggatagt gcgtaagtca ccagaaagac
     7441 acaactttac attttgtagc atctgattta cggcatcttg ccgctgttaa aaaatcaggc
     7501 acaatttctt gtgcctgatt gatattactt gacgggagcg ggttgttccg cgggtctggc
     7561 tttttcaagc ttcgccagat cgagtgcgat attcaccgtc tcatccagat aaggatccgg
     7621 ctcctggtaa tctttcgata gatcatccag cttcttcaac tccggtttac cttctcgttt
     7681 aaagcgttcg ttcaaacgcg ccagacgcgt cgcatcatct tcattattct ctttctcacg
     7741 cacagcgtag ttcagagaaa cgatattgcg cttgtccttc atagcgttga agcgcgcgat
     7801 atccttcatg atgttctgga actcaggatc tttcgcgata cgcgcattat gttccttcag
     7861 cagctccggt tcaaaggcag ttaaatctcc tgatttcaca taagtcgcgg catcgatgct
     7921 atcccacggc agcgcgttat cttcgaattt ctcacccgtt tccgtttctt cattacccgt
     7981 cggcatgatg atgtctggtg ttacgccttt acgttgcgta ctgccgccgt taacgcgata
     8041 gaatttctgg atcgtgtact gcacagaacc cagcgctggc cattcaggac gtaacatctg
     8101 atcgtaaata cggttcaatg aacggtattg ctgaacggtg cctttaccaa acgtcggttc
     8161 acccacaacc agcgcacgac cgtaatcctg cattgccgcg gcaaagattt ctgaagccga
     8221 agcactgaaa cggtcaacca gtaccaccag cgggccttta tagaacacct gcccgtcggt
     8281 atcgctgtct tcacgaacct tgccgttgtt atcgcggacc tgaacaatgg gacccgcagg
     8341 aataaacaga cctgagagcg ataccgcttc ggtcaacgcc ccaccgccat tgctacgcag
     8401 gtcgatgatc acactgctga cattctgttt ttccagtttc tgcagttgaa ctttgacatc
     8461 gtctgtcaaa cccacataga agcccggaat atccagcacg ccgactttct ctttaccgac
     8521 ggtcttcacc gacattttaa ccgcgcggtc ttcgagacga atacgttcac gggtcaacgt
     8581 tacagtacgg gtcttggtcc ctttaccagc aggtaaaatt tccagacgaa ctttactgcc
     8641 cttcggccct ttaattaagg caaccacatc atcaagacgc cagccaatca cgtcaaccat
     8701 cggcttgcct gtttgaccaa caccgacaat tttgtcacca acgctgatag ctttactctt
     8761 cgctgccgga ccacctgcca ccatcgaatt gataacggtg tagtcatcat ccatttgcag
     8821 cactgcgcca ataccttcca gtgacaaact catttcagtg ttgaactgtt cggtattacg
     8881 cggggaaaga tagttggtat gcggatcgat ttcacgcgca aacgccgtca ttgccagcga
     8941 gaaaacatct tcgctgttgg tttgcgccag acgacgaatg gcaaatttgt agcggcgtgt
     9001 cagggtttca cgaatttctt tatccgtttt tcctgtcagc ttcaggctta actcgtcgaa
     9061 tttgacttta ctgtcccact gcgcgttcaa ctcagcctcg tttttcggcc agggcgcttt
     9121 gctgcggtca aggttataag tgtcgttgcc ggtgaaatcc atcggctttt ccagtaccga
     9181 caaagcgtac tggtaacgtt caaaacggcg cttttgcgcc agattgtaga gatcgtagaa
     9241 aacgtcgagt ttgcctgaac gcagttcatc gcctaactcg gttttctttt tcgcgaactg
     9301 ttcaacatcg cttgccagca gcacattgtg gctgtaatcg agcagattca ggtagcggtc
     9361 aaagattttg gccgaaaatg cctgatcgag gtcgaactgg cgataatgag aacgggtgaa
     9421 gcgcgacgtt acgcgctcac ttaccgtcgc atgctgcgtc tcttccttta ataccggaat
     9481 ttgatcagca cgcgtgatat cttctacagc gaaggtctgg cctgctattg caagcaggcc
     9541 agctaacgcg gtaagcctaa aaaacatgtt catgcctggc ccggcctccg tttcagaaca
     9601 ccaggtgttc tgcgcgcaca atcaaagaca tacccgaatt cagctggacg cggacgccgt
     9661 ctttggtgat ttctaatacg gtggcatcca tcgcgttttg acccgctttc accttcaggg
     9721 cttgtccgac agtcagagct gaaatgtcag aaaccggggt gtgctgttct tcgcgaggtg
     9781 cttttactgt ttttggcgct ttctctaccg gcttttgcgc acgaggttta cgttcagcgc
     9841 cttctttgcg gcgtggcgtg gtcggacgtg gcttgcgttc gcggcgcggt gcgtcttctt
     9901 tctcaccagc agctgcggca gcttcgcgtt ttttcgcttg ctgttcagca cgctgtgcct
     9961 gaacacgcgc tttcgcttct tcaagctgct tgcgagcatg ctctacatgt tgctcgtcca
    10021 gctcaccgca tgggttgccg tcaagatcga cacgcgttgc gccgggttta acaccgtaaa
    10081 gataacgcca gctcgaagtg tagagacgta aagcggatcg taattgcgtt ttgctcaggt
    10141 tcatttcccc ggcaacacga tcgaccaaat cctgaaaaat accaattttc agcggacgcg
    10201 cttcaccttc cgcactgaaa cagtggggaa aacgttcggc cagaaacgcg attacttctt
    10261 tactgctatt caacttaggt tgattttcca tgaaatttcc tgattacaac ggacgtagcc
    10321 aacaagcgca ggcatgaaca ggcgtcatta taatgacgct accagtaaat gctacgttat
    10381 ccgttgatta tcctgcgacg ctcgcaaaga attttttgta atccgtcgtt gcaagcactt
    10441 tttcaagctg tgccacaagc tgacgtaagc cttgctcgtc ctcgtctgta aagcgagcga
    10501 agacagtact atcgatgtcg agaacaccaa taatctgatt tttcaccacc agcggcagaa
    10561 caatttcaga attactcgcc gcatcacagg caatatgccc gtcaaacgcg tgaacatcct
    10621 cgatacgctg cacttgattg cgggcaaccg ctgtgccgca caccccgcgc ccgacgggta
    10681 tccggacaca ggcaattttg ccctgaaatg gtccgagtac cagtgtatcg tcctcaagca
    10741 aataaaaacc tgcccagtta acgtcagtga gacgctcgta taacaacgca ctggtgttcg
    10801 caagcgttgc cagaaaactg gtttctcccg ccatcagcgc gttaaagtcg cgatttaaat
    10861 ccgcgtaaaa ttctgttttg ttcattactt aatcacttgg ttgtcttacc tggaactgcc
    10921 agcctattaa aataagcatt aaatgcgtta atgctcaaga tcattcccat catgggttaa
    10981 gattaatgtt aattcttatt acatttggca cgtcatggct cttaacacac cacaaattac
    11041 gccgacaaaa aagataacag tgagggcaat cggcgaggaa ctgccgcgtg gtgattacca
    11101 acgttgcccg caatgtgaca tgctgtttag cctgcccgag ataaattctc atcaaagtgc
    11161 ctattgtccg cgctgtcagg caaaaattcg cgacgggcgc gactggtcgc taacgcgcct
    11221 ggcggcaatg gccttcacca tgctgttgct tatgccgttt gcctggggcg aaccgctgtt
    11281 gcatatctgg ctgttaggca ttcgtattga cgccaacgtc atgcaaggca tctggcaaat
    11341 gaccaaacag ggcgatgcga taacgggatc aatggtcttt ttctgcgtta ttggtgcccc
    11401 cctcattctg gtgtcctcca tagcatattt atggtttggt aaccgactgg gaatgaattt
    11461 acgtccggta ctgctgatgc ttgagcgact taaagagtgg gtaatgctgg atatctacct
    11521 ggtcggcatt ggcgttgctt ctataaaggt acaggattat gcccatatcc aggcgggtgt
    11581 cggcttgttc tcttttgtgg cgttggtgat tttaacgacg gtgacgttgt cacatcttaa
    11641 tgttgaggag ctgtgggagc gatattatcc gcagcgcccc gctacgcgta gggacgagaa
    11701 acttcgtgtc tgtcttgggt gccattttac cggctatcct gatcagcgtg gtctctgccc
    11761 gcgttgccat atcccgctac gcctgcgtcg tcgtcatagt ttgcaaaaat gctgggcggc
    11821 gctgttagcg tcaatcgttt tgttgttacc agccaaccta ttgcctattt ctatcattta
    11881 tctgaatggt ggccggcagg aagatacgat tctttccgga attatgtcgc tggcaagtag
    11941 caacattgcg gttgcgggaa tcgtgtttat cgccagtatt ctggtaccgt ttactaaagt
    12001 gatcgtcatg ttcactttac tgttgagcat tcattttaaa tgccagcaag gtttacgcac
    12061 acgcattctg ttactgcgga tggtgacctg gattggacgc tggtcgatac tcgacctgtt
    12121 tgtcatatct ttaaccatgt cgctgattaa tcgcgatcag atcctcgctt ttactatggg
    12181 accggctgcg ttttattttg gcgctgccgt aattttgact attcttgctg tggaatggct
    12241 ggacagccgc ttactttggg atgcacatga gtcaggaaac gcccgcttcg acgactgaag
    12301 cgcagattaa aaataaacgc cgtatctcac ctttctggct gctgcctttc atcgcgctaa
    12361 tgattgccgg ttggctgatt tgggacagtt atcaggaccg gggtaatacc gtcaccatcg
    12421 actttatgtc ggcggatggt attgttccgg gccgtacgcc tgttcgttat cagggcgttg
    12481 aagtcggaac agtgcaggat atcagcctca gcgacgatct tcgtaagatt gaagtcaagg
    12541 tcagcatcaa gtccgatatg aaagatgcgc tgcgcgaaga gactcagttc tggctgatga
    12601 cgccaaaagc atcgttggca ggtgtctccg ggctggacgc cctcgtcggt gggaactata
    12661 tcggcatgat gccgggtaaa ggtaaagagc aggatcactt tgtcgcgctc gatacccaac
    12721 cgaaatatcg gctggacaat ggcgatctga tgatccacct gcaagccccc gctctcggtt
    12781 cgctgaacag cggttcattg gtctatttcc gcaagatccc ggtgggaaaa gtctacgact
    12841 atgccatcaa tcccaacaag caaggcgtgg tgattgatgt cctgatcgag cggcgtttta
    12901 ccgatctggt gaaaaaaggt agccgtttct gaaacgtttc cggcgttgat gccaacgtca
    12961 gtatcagtgg cgcgaaggtg aaactggaaa gtctggcggc actggttaac ggtgcgattg
    13021 ccttcgattc acctgaagag tcgaaacctg ccgaggcgga agataccttt ggtctgtatg
    13081 aagatctggc ccacagccag cgtggagtaa taataaaact ggaactgccg agtggagcag
    13141 gattaaccgc cgactcgacg ccgttaatgt atcaggggct ggaagtcgga cagctgacta
    13201 aactggattt aaatcctggt ggtaaagtca ctggggaaat gaccgttgat ccctgcttcg
    13261 tgaaaatacc cgcatcgaat tacgcaaccc gaaattatcc cttagcgatg ctaatctcag
    13321 cgccctgctg accggaaaaa cctttgagtt ggtgcccggc gatggcgagc cacgcaaaga
    13381 gttcgttgtt gtgccgggcg aaaaagccct gctgcaagag ccggacgttc tgacactgac
    13441 cctgacagca ccggaaagtt acggtattga tgcgggtcag ccgctcattc ttcacggcgt
    13501 gcaggtaggc caggttatcg atcgtaaact caccagcaaa ggcgtcacct ttaccgtcgc
    13561 catcgagcct cagcatcgag aactggtaaa aggcgatagc aaatttgtcg tcaacagccg
    13621 tgtcgacgtg aaggtggggc tggatggcgt tgagtttctc ggtgccagcg cctcagaatg
    13681 gattaacggc gggatacgta ttctgccggg cgataaaggc gagatgaaag ccagctatcc
    13741 actgtatgcc aatctggaaa aagcgctgga gaacagcctt agcgatttac ccaccacaac
    13801 catgagtttg agtgcagaga cgctgccgga tgtgcaggca ggatcggtag tgctgtaccg
    13861 taaatttgaa gttggtgaag ttattaccgt gcgtccgcga gctaacgcgt tcgatatcga
    13921 tctgcacatt aaaccggaat atcgcaacct tttgaccagc aacagcgtgt tctgggcaga
    13981 aggcggggcg aaagttcagc tgaatggtag tggcctgacc gtacaggcat ccccgctctc
    14041 cagagcatta aagggggcca ttagcttcga taacctcagt ggtgccagcg ccagtcagcg
    14101 taaaggcgac aagcgaattc tgtatgcttc cgaaacagcg gcccgtgcgg ttggcaggca
    14161 gattacgctt cacgctttcg atgccggaaa actggcggtc gggatgccaa ttcgctatct
    14221 cggtattgat atcgggcaaa tccagacgct ggatctgatt accgcacgca atgaagtaca
    14281 ggcaaaggcg gtgctctatc cggaatatgt ccagaccttt gctcgcggtg gtacgcgctt
    14341 ctcagtggtc acaccgcaaa tctcggcagc tggcgttgag catctagata ctatcctcca
    14401 gccgtatatc aacgtcgaac caggccgtgg caatcctcgt cgcgactttg aattagaaga
    14461 ggccaccatt actgattcgc gttacctaga tggtttaagc attattgttg aagcgccgga
    14521 agccggttcg ttaggcatcg gtacgcctgt gctgttccgt ggtctggaag taggtacggt
    14581 taccggaatg acgctgggga cattgtcaga tcgcgtgatg attgcgatgc gcatcagtaa
    14641 acgctatcaa cacctggtgc gtaacaattc cgtcttctgg ttggcatcgg gttacagtct
    14701 ggactttggt ctgacgggcg gcgtagtgaa aaccggcacc tttaaccaat ttatccgtgg
    14761 cggcatcgcc ttcgccacgc ctccgggtac gccacttgca ccgaaagccc aagaaggcaa
    14821 acacttcctg ttgcaggaaa gtgaaccgaa agagtggcgt gaatggggaa ctgcgcttcc
    14881 caaataatgc ccactgctcc ggcgtgcctg cgccggagcg cttatgctaa actgcgcgcc
    14941 tgtttttttg ccagtggtac atgctcgtgg cccaacacac cgtttatttc ccggacgcgt
    15001 ttctgacaca aatgcgcgaa gctatgcctt cgacgctctc atttgatgat tttcttgccg
    15061 cctgtcagcg cccgttgcgc cgcagcattc gcgttaatac gctgaaaatc tccgttgctg
    15121 atttcctgca attaaccgct ccttatggct ggacgcttac gccaattccg tggtgtgaag
    15181 aaggtttctg gattgaacgc gacaatgaag atgcattgcc attgggtagt accaccgagc
    15241 atttaagtgg cctgttttat attcaggaag ccagttcaat gttgcccgtc gccgccttgt
    15301 ttgctgacgg taatgcacca cagcgggtga tggatgtcgc tgctgcgcca ggctcaaaaa
    15361 cgacgcaaat tgccgcgcgg atgaataacg aaggggcaat ccttgccaat gagttttccg
    15421 ccagttgggt aaaagtgtta catgccaata tcagccgctg tggcatcagt aatgttgcgc
    15481 tcacacattt tggtggccgc gtgtttggtg cggcagtgcc agaaatgttt gatgccattt
    15541 tgctggacgc tccctgctcc ggcgaaggcg tggtgcgtaa agatcccgat gcgctaaaaa
    15601 actggtcacc cgaaagcaat caggaaatcg cagctaccca acgggaactg attgacagcg
    15661 cctttcatgc attacgtcct ggcggtacgc tggtttactc gacctatacc ttaaaccggg
    15721 aagaaaacga agccgtttgc cggtggctga aagagactta ccccgacgaa gtagagtttt
    15781 taccacttgg cgatctcttc cctggtgcaa acaaggcgct gaccgaagaa ggttttttgc
    15841 atgttttccc acaaatttac gactgcgaag gcttcttcgt tgctcgtctg cgtaaaactc
    15901 aggcgatccc cgccttaccc gccccaaaat acaaagtggg caatttcccg ttcagcccgg
    15961 tgaaagatcg cgaagccgga caaattcgtc aggcggctgc aagtgttggc ttaaactggg
    16021 atggaaacct gcgactctgg caacgcgaca aagaactgtg gttgttcccg gtaggcattg
    16081 aagccctgat cggtaaagtc cgattttctc gcttagggat taaacttgcc gaaacgcata
    16141 acaaaggtta tcgctggcag catgaagcgg ttattgccca tgcctccccc gacaatgtaa
    16201 acgcttttga actgacaccg caggaagcgt aggagtggta tcgcgggcgc gatgtttacc
    16261 cgcaagccgc gccagtggcg gatgacgtgt tggttacttt ccagcatcag ccgattggtt
    16321 tagccaaacg gattggttcg cgactgaaaa acagctaccc gcgtgaactg gtgcgggacg
    16381 ggaaactttt taccagtaac gcatgacagc gcacaaaaaa agcgcacttt ttgactggca
    16441 cattcgccgg cctcaactag gctgaaaaat tgtgcgatcg gactggtcgt actacaatca
    16501 gcagctaaat ggagagcacg aagatgaaaa ccagtgtgcg cataggcgct tttgaaatcg
    16561 acgacggcga attacacggt gaatcgccgg gtgatcgaac gttaaccatt ccttgtaaat
    16621 ctgaccccga tttatgtatg caactggatg cctgggatgc tgaaaccagt atccctgccc
    16681 tgctaaatgg cgaacactct gtcctttacc gtacccgtta cgatcaacaa tctgatgcct
    16741 ggattatgcg tcttgcctga tccaaaaaga acccgtcggc atggcgggtt atttgtcctg
    16801 gttattcccc cgttgtaaaa tctctcctaa acttaacggt acggcaccac acttcgggga
    16861 tgaaatgttc gcgctggtac tttttgtttg ctacctggat ggcggttgtg aagatattgt
    16921 tgtggatgtc tacaacacgg aacagcagtg tctttattct atgagcgatc aacggatccg
    16981 ccatggcagt tgttttccga ttgaggattt tatagatggt ttctggcaac cagcacagga
    17041 gtatggtgat ttttaattat tgcagttgca caagagtcag ttagccccca aagacagcac
    17101 cggtatcaat ataatgcagg ttgccaatat ccacgcgatg tcgcaacggt gtatgaccaa
    17161 accagaaatg atcagcacct gtaattccct gcccttttgg gcgttcacct aatcgcgagc
    17221 ggctccacaa gacctgatgc aaatcaacgt ccttttgcca ttcat
//
LOCUS       DAJTSI010000085        17200 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000085 DAJTSI010000000
VERSION     DAJTSI010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.085"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..329
                     /locus_tag="OO327_002533"
     CDS             <1..329
                     /locus_tag="OO327_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR6019035.1"
                     /translation="RGRARRKEYWMFILVNIIFTFVLGLLDKMLGWQRAGGEGILTII
                     YGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFNCQAGTPGENRFGPD
                     PKLEQE"
     gene            complement(536..1432)
                     /gene="yhaJ"
                     /locus_tag="OO327_002534"
     CDS             complement(536..1432)
                     /gene="yhaJ"
                     /locus_tag="OO327_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCR6019036.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            1537..2238
                     /locus_tag="OO327_002535"
     CDS             1537..2238
                     /locus_tag="OO327_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR6019037.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2261..2425
                     /locus_tag="OO327_002536"
     CDS             2261..2425
                     /locus_tag="OO327_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019038.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2637..3947)
                     /locus_tag="OO327_002537"
     CDS             complement(2637..3947)
                     /locus_tag="OO327_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR6019039.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AITDAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3975..5306)
                     /locus_tag="OO327_002538"
     CDS             complement(3975..5306)
                     /locus_tag="OO327_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR6019040.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLVWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(5581..6945)
                     /gene="tdcG"
                     /locus_tag="OO327_002539"
     CDS             complement(5581..6945)
                     /gene="tdcG"
                     /locus_tag="OO327_002539"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6019041.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(7017..7406)
                     /locus_tag="OO327_002540"
     CDS             complement(7017..7406)
                     /locus_tag="OO327_002540"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR6019042.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7420..9714)
                     /gene="tdcE"
                     /locus_tag="OO327_002541"
     CDS             complement(7420..9714)
                     /gene="tdcE"
                     /locus_tag="OO327_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCR6019043.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9748..10956)
                     /gene="tdcD"
                     /locus_tag="OO327_002542"
     CDS             complement(9748..10956)
                     /gene="tdcD"
                     /locus_tag="OO327_002542"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR6019044.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10982..12313)
                     /gene="tdcC"
                     /locus_tag="OO327_002543"
     CDS             complement(10982..12313)
                     /gene="tdcC"
                     /locus_tag="OO327_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR6019045.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12335..13324)
                     /gene="tdcB"
                     /locus_tag="OO327_002544"
     CDS             complement(12335..13324)
                     /gene="tdcB"
                     /locus_tag="OO327_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR6019046.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13423..14361)
                     /gene="tdcA"
                     /locus_tag="OO327_002545"
     CDS             complement(13423..14361)
                     /gene="tdcA"
                     /locus_tag="OO327_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR6019047.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            14551..14895
                     /gene="tdcR"
                     /locus_tag="OO327_002546"
     CDS             14551..14895
                     /gene="tdcR"
                     /locus_tag="OO327_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR6019048.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            15151..15691
                     /locus_tag="OO327_002547"
                     /pseudo
     CDS             15151..15691
                     /locus_tag="OO327_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15713..16060
                     /locus_tag="OO327_002548"
     CDS             15713..16060
                     /locus_tag="OO327_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019049.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            16229..16879
                     /locus_tag="OO327_002549"
     CDS             16229..16879
                     /locus_tag="OO327_002549"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019050.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELT
                     KEIYKSSSKINNCLNRIKLPEMKEFSSKKTYDYIDTIIEDYENIKENAYLVVPQINYT
                     MDLNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
BASE COUNT         4721 a         4382 c         3852 g         4245 t
ORIGIN      
        1 tccggggtcg tgcgcggcgc aaagagtact ggatgtttat tctggtcaac atcatcttta
       61 cgttcgtgct ggggctgctg gataaaatgt taggctggca acgcgctggt ggcgaaggca
      121 tcctgacgat aatctacggt attctggtgt ttttaccgtg gtgggcggtt cagttccgcc
      181 gcctgcacga caccgaccgc tcggcgtggt gggcactgct attcttaatc ccgtttatcg
      241 gctggcttat tatcatcgtc tttaactgcc aggcgggtac gccaggcgaa aaccgctttg
      301 gaccagatcc aaaactggaa caagaataat gattaagcac cttgtaaaag gtgcttcaaa
      361 ttgttgacaa agtgcgcttt gtttatgccg gatgcggcgt aaacgcctta tccagcctac
      421 aaattgccaa aattcaatat attgcagtga ctgcgtaggc ctgataagcg tagcgcatca
      481 ggcagttttg cgtttgtcag cagtcagaag caccttgtga aaggtgctta atagattatt
      541 ttccagcgaa caactttgga atttcccgca gacaccagga tttcgcttcc cccatactgt
      601 cacgacgcca ggccataata atatcgatct cgctggtcga ttccgggctg acgacacgca
      661 accgcccttc cgcaatatct ttttcgacca tcggatacgg catcgtcgcc acgccaagcc
      721 ccgccagtaa tgcctgacgt ttatcttcaa tcgtgctcac cgttaagcgc ggctgtttgt
      781 ccagcagctg tacggtcaac accgggcgct cacgagcggt atccgctacc gcaattccac
      841 gatatttcac acgcgtcact tcagataacg gttccggctc ctgatgaatc gggtgatcag
      901 gcgcggcaac gtagacgttc attaacgtat agagcttgcg cgagttgatc tccgacgagg
      961 aacgaaaatg catatccggc gcgataacga tatccgcccg cccctgctcc agccgttccc
     1021 acgcccccgc cagcacttct gtgatgattg ccagttgggt attggctttt gccgccagtt
     1081 tgtcgattaa cgggaaaaag gcaggtgtcg gtaccagcgc ttcggtcaca atggtgagat
     1141 gcgtttccca accgcgcgcg agagcttccg catcagtagt cagtttatct gcggcttcca
     1201 gcaaaacgcg ccctcgctcc agcaacatcc tcccgacatt ggtgaatttg gtgcgatggc
     1261 ccgagcggtc aaacagcacc acatccagct cttcttccag tttttgcatg gtgtagctaa
     1321 gtgcggaagg cacgcgtccc agctcatccg ctgccgccgc aaaactgccc cggcgatcga
     1381 tcgcatccat aacccgtagt gcttccagcg ttaatgccct ttctttggcc atttcgttct
     1441 cattcaggaa atttgaacat accgggcaga atatctggct aacaatgtag cgtccagccc
     1501 cttaccataa aaggaagtaa agagaggtca agaattatga ttactacccg aactgccagg
     1561 cagtgtggac aagcagacta cggatggttg caggcccggt atactttttc ctttggacac
     1621 tacttcgacc cgaaattgtt aggctatgcc tccctgcgtg tgcttaacca ggaagtgctg
     1681 gccccaggtg ccgcctttca gccgcgaacc tatcccaaag tcgatatttt aaatgtgatt
     1741 ctggatggag aagcagagta tcgcgacagc gaaggcaatc atgttcaggc cagcgccgga
     1801 gaggcgttgc tgctctctac ccagccgggt gtcagctata gcgaacacaa tctcagcaaa
     1861 gacaaaccgt taacgcgaat gcagctttgg ctggatacct gcccgcagcg agagaatccg
     1921 ctgattcaaa agctggcgct taatatggac aagcagcaat taatcgcctc gccagagggt
     1981 gcgatgggaa gcctgcaatt acgccaacaa gtgtggctgc accatatcgt gctcgacaaa
     2041 ggcgaaagtg cgaatttcca gttgcatggg ccacgcgcgt atttgcaatc gattcacggg
     2101 aaatttcatg cgcttacgca tcatgaagag aaagcggcgc taacctgcgg tgatggggcg
     2161 tttattcgtg acgaggctaa cattacgctg gttgccgatt ccccactgcg cgctttgctg
     2221 atagatttgc ctgtctagtt gtttttacag ggagatgatg atgagtaaaa aatcgaccaa
     2281 aaagcgccag ccagcgaagc ccgtggtggc gaaagaacct gctcgcaccg ccaaaaattt
     2341 tggctatgaa gagatgttga gcgagctgga agctatcgtc gcggatgctg aaacgcgttt
     2401 agccgaggat gaagctaccg cgtaaagaca ggttgccgga tgcggcgtaa acgccttatc
     2461 cggcctacaa aacacaacaa catcaacaaa ttaaacaccc cgtaggccgg ataagatgcg
     2521 ccagcatcgc atccggcatc aggtacaaaa cacaacaaca tcaacaagtt gagcaccccg
     2581 taggccggat aaggtgcgcc agcatcgcat ccggcacaat actcaaaacc tggcgtttat
     2641 ctggccttgc tcgccataat ctcgataatc tgccgatccg tttgctgcat cgaatggctt
     2701 gctaacgcac acaggttggc aatcgactgc tcaacatcat gcgccacgat cccttcattg
     2761 ccggtcacgg cggtatcatc cagcgccatt aacaccgctt tccacgcagc cgaagcactg
     2821 gtcgaaacct tcatcgcgca gctgttcgac gcaccatcgc aaatcatgcc gctgacatcg
     2881 ccgatcatac tgctgatcgc catcgagata gtttcataac gcccatccac cagccatgcc
     2941 atcccggcgg cggcccccat tgctgcggtc gttgcggcac aaagtgcaga caaacgcggt
     3001 aactggttat ggatgtaaat tgcgctcaaa tgagaaagca tcagcgcacg cgccagtcgt
     3061 tcatcatccg ctccgaagtg ttctgctaca accaccacag gcattattgc agtgatcccc
     3121 tggttacccg agccggagtt actcatagcc ggaagcgtag cgccgcccat acgcgcatcg
     3181 gatgccgcgc tggtacgaat cacaatggat gaagagagat ctttcgccag caaaccgcgc
     3241 tcgcactgtt tttccagcgt cgcgccaata tgcagccccc acttaccgct caaaccttcc
     3301 tgcgataacg cacaatttag cttcgcggaa tcgagaataa agcggatcgc cgcaaatggg
     3361 acttcattga cgaacttcag gatctcagcc agcgtcgttc tggaaagcac cgtcagcggc
     3421 gactcttgct cgccctctgc cacacacgcc tggtgggtaa acaccacacc attgtgcgtc
     3481 tcaatatgca caatgttggt atgcccgccg acgatggtga cacacgccca cttctcaccg
     3541 ttccagactt tggcgcgtga gaagaggatt tcattgcaag gttcctggat cttaacagag
     3601 actttccccg ccgccagcag tgctttggca tcggtaattg cctgcgcagt tgcgtctttc
     3661 agcacttcca gcccggcgtt ggcatttcca cctaacgccc ccagcgccgc cgcaatcggc
     3721 agccccacca ttcccgtgcc gggaacggtg acgcccagac cgttcttcat cagatttggc
     3781 gaaacccagg cttctacacg ttcaaccgga ccttccagtt ctgccgcagc aaccgccgcc
     3841 gccagcgcca gtgaaatcgg ttcagtacat cccagcgccg gttttacttc ctcctgaacg
     3901 gcgaggatgt aacgctgcca taacggattt aaagtcgaat caaacatatt aaaaacctta
     3961 aaatttcagg taaatcaaga aaatgccagg aacagagaaa cacaaagcaa caaaccagtg
     4021 acgataatca ggtacagaga catccctttg tatttgtgca atgccggtac tttgtaaacc
     4081 agccacgccg ggatcaggca ccctaccatg ccgaaaatcg ggctacagat agaggtgaag
     4141 ctcaacaccg gtgcgttcag tacgatggcg ctccagacca gcaaaatggc gaaaatcatg
     4201 atgccgcgct gaacaagatt ttcgttaatc ttctcggcag gcatcttgcg acgcaggatg
     4261 ttcattacga tcccttgcgt tgcttcgcga aagcctaaat agacgccaaa gaatgcagtc
     4321 atgacagcaa agatattgag aatgacgctg accactttca cccacgctgc gccgtcaccg
     4381 ctaataaact gtgcggcaat cgccagcgcg gaaatattct gctcgtaggc ttttaccgct
     4441 tcgtcatgtc ccatcgccag cgtgaacgac acggcgtaga aaaagacggt gacaaacaaa
     4501 atgccaaacg cgatattcat tgcccgcaat gctttatgcc gcgccacttc aattgatttt
     4561 tcccgcgagc gataagagat caccatcggg cttaacgtct ggataaacag aatcgacgtc
     4621 agggtaaacg gcagcgtaat aatggcgttt ttcaccagca gccccagcgg cggtagcgaa
     4681 ccgacgttat acagatgcca cattcccacc atcgacacgc ccagcgcagc gactaccagc
     4741 agcttggtca gcaccatgtc ggtcgaaatt ttgaataaca atttctcgcc gcgtgaagag
     4801 atcgccacca gaatgcaaat cagtaccaga ccataaaagg gactgtctga cagcaaccct
     4861 tccgtcacgc cgaaggtatg cagataggaa gcactatcgt tggtgatggc ggtggaatag
     4921 acgaacatcc aaatcaccag cattacgaaa tagagcgcac ctaacaggat gccccagttt
     4981 ttacctaaat aaccgctaat gacactcggg taatctttac attctggtga ttctgccagc
     5041 gtattaataa acaaccgctg aaacagatac attgccgggt aaccaatcac cgatgagagc
     5101 aaaaataccc acaatcccat caaaccgacc tgcaccggga gaaaaacaat ccccgcgcca
     5161 atcgccatcc cgatactcat aatcacccag ccggtgtcag tactgtcgaa tttgatcgct
     5221 tctcgccact cgctctcact cattccagca cgacccgccg gggtcgaagc gtctgcaatg
     5281 acgcctttat tcgatgcaat ttccataatt tctcgctcaa tattttgtag ggcttattat
     5341 ttttttccga gccgcatcaa ggcgatatgc ggtctacgtt tttgcaggcg agttattaga
     5401 ataaagaaat gatacgcgcg aggtcagaga aaatcttcac aatcaagact ttaaaataac
     5461 ggagatggat aagaattttc tactaaatta atcacaagag aatattcatt ctctatttgc
     5521 ggcagatcac aaaaaaaagg tgcacatttg tgcacccaag gatgaaagct gacagcaatg
     5581 tcagccgcag accactttaa tggccagtcc tccgcgtgat gtttcgcggt atttatcgtt
     5641 catatctttg ccggtttcat acatcgtctc gatcacttta tcgagtgaaa cacgcggtgc
     5701 cgaggtgcgg cgcatcgcca tccgcgcggc gtttactgct ttcacggcat taatggcatt
     5761 acgttcaatg cacgggattt gtacctgtcc ggcaaccgga tcgcaggtca gcccaaggtt
     5821 atgctccatc gcgatttccg ccgcattgca tacctgcgcc ggactgccgc ccagtagttc
     5881 agttaaccct gccgctgcca ttgaacaggc cacgccaatc tccccctgac agccgacctc
     5941 cgcgccagag atggaggcgt tcattttata cagcgcgcca atagcccccg ctgccagaaa
     6001 atagcgggca attgaccgct cgtttaccgg acgacggaac ttatcgtaat aagccagcac
     6061 tgccggaata atgccgcacg caccgttagt cggtgccgtt accacgcgcc cgccagctgc
     6121 gttttcttca ctaaccgcca gcgcgtacat gttgatccag tcgatgacat tcatcggatc
     6181 gttagagatg ttatcgctag aaaccagctg acgacgcagc gctacggcac gacacggcac
     6241 attgagcgga ccaggcagca cgccttcagt gttcatcccg cgttcaatac cgtcatgcat
     6301 cacttgccag atacgggcaa accctgcgtc aatttccgct ttgctgcgca gcgccagctc
     6361 gttgtgcatc atcaggccag atatagacag gccgttgtaa tcacacattt tcagcagttc
     6421 acctgctgag tggaaatcgt aaggtacgga cgtttcgaca tcgtgcgaca ggccgaagtg
     6481 ttcttcttcg acaataaacc cgccgccgac agagtaatag gttttactta atagcgcttc
     6541 ctgtcccttc caggcagtga tccgcattcc gttctcatgg cgaggcaaca tttcgggatg
     6601 gaagataatg ttctttgcta caggaaaatc aacaatatgc gcaccagatg ccactggcag
     6661 ccgcccgctg cgcgttacta actctataaa tgcagggatc tcatcaatga caacatcctg
     6721 cggactgttt cctgccagtc ccatgataat ggcgacatcc gtggcatggc ctttgcccgt
     6781 cagtgacaac gacccgtaca gatcgaccac aatatggctc gtcgcggtta ataagccgct
     6841 actttccagc cgatcaataa aactttttcc ggcattcatt ggccccacgg tatgcgaact
     6901 ggagggacca atcccaattt tgaaaatatc gaatgcacta atcatatcca caccctcgga
     6961 ttgccgttca gtgaagtgga gcggaacgac cttgcgaccg tcccgctcac gaggctttac
     7021 gcactacgta ctgcgatagc ttcaatttcc agcttcacat ctttcggcaa acgcgcaacc
     7081 tgcacacagc tccgggtcgg gtaggtcgcc tgatgctcat cgaagaactg cttatagact
     7141 tcattgatgg tggcaaaatc attcagatcg gtgatgaaca cggtcatctt gatgatatcg
     7201 cccacgctca gcccggcagc aaccacgatc gctttgacgt tttcgaggct taaacgcgcc
     7261 tgatcttgca catcagccgg gatctcaccg gtctgcgggc aaaccggaat ttgcccggag
     7321 gtgaagacca tgctgcctaa atcaacgccc tgaacataag ggccgattgc gcctggggca
     7381 cgttgcgttt cgataatctt tttcatacat cctccggcgt cagagcgcct gggtaaaagt
     7441 acgtgaaata acatcctgtt gctgttcacg ggtcagtgcg ttgaagcgca cggcgtagcc
     7501 agagacacgg attgtcaggt taggatattt ttccgggtgc tcgatggcat ccagcagcat
     7561 ttcccgattc attacgttga cgttgaggtg ttgaccgcct tcgacatccg cttcgtggtg
     7621 gaaataccca tccagcaggc cgacaaggtt ggttttacgt actggatctt ctttgcccag
     7681 cgccgcagga acgattgaga aggtgtacga gatcccatct ttggcgtagg tgaacggcag
     7741 tttcgccacc gacgtcaatg aggccacggc acctttgcgg tcacgaccat gcatcgggtt
     7801 agcgcccggc gcgaacggtg ttccggcgcg acgaccgtcc ggcgtattac cggttttctg
     7861 gccgtacacc acgttagaag tgatagtcag aatcgactgg gtagggacgg cgttgcgata
     7921 ggttggcagc gctttaattt tcttcataaa gcgttcaacc aggtcgcagg caatgctgtc
     7981 tacgcgctcg tcgttgttgc cgtactgcgg atattcaccg tcgatttcaa agtccaccgc
     8041 caggccgttt tcgtcacgga ttggtttcac gcgggcatat ttgatggcag acagggagtc
     8101 cgtcgccacc gacaggcccg cgatgccgca tgccatagtg cgatagacat cacgatcgtg
     8161 cagcgccatc agcgaagctt cgtagctgta cttgtcgtgc atgtagtgaa tgatattcag
     8221 cgcgctgatg tactgcaccg ccagccagtc catgaagtga tcgaggctgt ccatcacttt
     8281 gtcgtaatcc agcacgtcgt ccatcagcgg tgctgttttc ggcccgacct gaatcttcag
     8341 cttctcgtcc accccgccgt taattgcgta gagcagcgtt ttcgccaggt tagcgcgtgc
     8401 accaaagaac tgcatttgct taccaatcac cattgggctg acgcagcagg caatcgcgta
     8461 atcgtcgctg ttgaagtcag tacgcatcag atcgtcattt tcatactgca aggaagaggt
     8521 gacgatcgac acctgcgcgg catatttttt gaaggcaatc ggtaattctt ccgaccaaag
     8581 aatggtcagg ttaggttccg gtgccggccc catagtgtgc agggtgtgca aatagcggaa
     8641 ggagtttttg gtcaccagcg tacgaccgtc cagccccatc ccgccgatca cttccgtcgc
     8701 ccagattggg tcgccggaga acagcgaatc aaattccggt gtacgcagga agcgtaccat
     8761 acggatcttc atgatgaagt gatcgatcag ttcctgtgcc tgctgctcat tgaggacgcc
     8821 agctttaaag tcgcgctcaa tgtagatatc caggaacgat gccgtgcgcc ccagcgacat
     8881 cgcgccgcca ttttgcgatt tcactgccgc cagataagcg aagtagagcc actgcaccgc
     8941 ttcctgtgca ttctgcgccg ggcgagagat atcaaagcca tatttcgccg ccatttcctg
     9001 aatctgcaac agcgcatgac gatgctctgc cagctcctca cgcagacgga tggtggcttc
     9061 cagatcctcg cctttttcca gacgagactg gagatcggca aattgcagtt cgcgttcacg
     9121 caccagataa ctgatgccat acagcgctac gcggcgatag tcaccgataa tgcgcccacg
     9181 accatagcca tctggtaaac cggtcagcac gccagattta cggcagcgca gcatatccgg
     9241 tgagtaaaca tcaaatacgc cctggttatg ggttttacgc agatcggtaa acagatattc
     9301 aaattcactg tccatttcac ggccataggc gtggaatgaa cttttaatca tattaatgcc
     9361 accgaacggg tgtagcgcac gtttcaacgg cgcatccgtt tgcaggccaa caattttttc
     9421 cagcggctgg ttaatatatc ccgcatcatg agcggtaatt gtggtggcaa tattggtatc
     9481 gaaatcaacc ggcgcgtggg ttgcgttttc gatacggatg ccttccatta ccttttccca
     9541 caattccgtg gtggcaggcg tcgcttcggc gagaaaagat tcatcgcctt catacggtgt
     9601 atagttatgt tgaataaaat cgcggacatt aatttcgctt ttccagtccg tacctttaaa
     9661 gccaagccat gcgtcagcgt acagcttatc gctggtatca atatctacct tcatgaaaaa
     9721 taatctctct acaatacttc aactaaatta tgcaaattct gcgggcgcgt taactttacc
     9781 taaatgaatg gcatccaaag caatcatttt ttcttcgtta gtcggaataa cgacgcaaat
     9841 gacatgcgca ttttcactgg aaacaattcg ctcgccaaaa gagttagaac gattattcat
     9901 ttctgtatca atctctacgc ctaatacagc caaatgttcc atgaccagac gacgaattaa
     9961 acttgaattc tctcctattc cgccggtgaa tataattcca tccagccgac gtaatgaagc
    10021 tgcatgtccg gcaatatgac gggcaattcg gtgaacaaag gttttaattg ccagttgcgc
    10081 gcgttcgtga ccttcatgcc aggctttttc cagaacacgt aaatccgaag aaagaccgga
    10141 aatacctaat aatcccgact ctttattcac tacgcgttcc aggtcaccca ggctctggtt
    10201 ggtttggctg gcgacccagg acatcgcacc aaagtcgaca tcgccactgc gggtacccat
    10261 catcaagcct tccagcggcg tcattcccat tgaggtgtca acactctgac cgttgcgaac
    10321 tgcgcagatt gacgcgccat tgccaagatg cgccacaacc aggccggagt catcttccgc
    10381 cagattcagc agcgaatgtg cgcgctggga aacatagcgg tgcgacgtgc cgtggaaacc
    10441 ataacggcgt acacctaact cttcataata tttccacggc aggccgtata aataagcttc
    10501 cggagccatc gtctggtgga aactggtatc aaataccgcc acctgagtta cgcccggaaa
    10561 taattgctgc gccgattcaa taccacttaa atttgcgtaa ttatgcaggg gtgccagtgg
    10621 agaaacgcga cggatattat caatgacttc atcggtaata atggcggact cggtaaaaat
    10681 actgccgccg tgagcgatgc ggtggccaat taaggccacg ctgtcattta aattccgttt
    10741 ttccagttca aatgcaattg ccttcaatgc accttcgtag ctgtggtgag ccagcggtgc
    10801 tggctctccc ccatttacgg ataagaatgc attttccgag ttaataccgt cggcaatacc
    10861 tgacattaat acttcacaat cgctggcatc gagtacggaa aacttaatcg aagacgaacc
    10921 acagttaata accaaaacaa ccggaaattc attcatctct tttctcatcc tgagttacgg
    10981 attaaaacag tttgtatacg atgttcagga tggtcagcag accaatcacg gtaacaaaca
    11041 cgttatccag acgaccacgg tatttcgcca gagacggcgc tttacggata gcatacatcg
    11101 gcaacaggca cagcagggat gcgataatcg gtgcgcccat ggcttcaatc aggtcgagga
    11161 tgttcgggtt ggcgtaggca acaacccagg tggagcccat gatgaagatc atgctgagag
    11221 tattcagttt acccagcgac actttggttt tgtcaccttt ataaccgaac ttcagaatca
    11281 gaccattcaa gccttccagc gtccccagat agtgaccgaa gaaagatttg aagatagcca
    11341 cgagtgcgat gatggaagcc gcatattcca gtgtaatcgc gaacgttgtt ttcgtaccgg
    11401 tcatggacgc aaagtggtta gccagataag aaagcactgg aatattctgc gctttggctt
    11461 ccgccatgtt ggccggagac agagtaaaca ggcagctaaa ggcaaagaac atcaccactg
    11521 caaccatcag catgctggca cgagaaatga tttgggaaca tttacgttcg gtgaagtcgc
    11581 gaccgaagtc tttctcatac tcttcacgtt tagaaaccac gaaggaagag acgattggcg
    11641 agaagttaaa ggagaaaacc atgatggaaa tccccagcca gacagtgatc aggataccgt
    11701 catgaccggt taacgacagc gaaccgaggt caacctggtc gataactgca gagttccagt
    11761 aagggatcag cgacagagaa atcagcacca ggctggcgat aaacggccat accaggtagc
    11821 tcatcacttt aaccatcaga tccttaccaa accagatgac gaaagccatc agcagcaaca
    11881 ggaacagcgc cacaaagccg cgattcagcg gtgcaaatcc gagctggttt tcccagaacg
    11941 tcataaaggt attggtaata gtaacgccat aaatccacag cagtgggcaa atcgcgaaga
    12001 agtacaggaa cgtgataacc acgccgccag ttttaccaaa atgctcttcc accgtttccg
    12061 taatgttgcc ggaagggtta gagccagaaa gacacagacg cgccagcgcc cggtggcaat
    12121 aaaatgcgat ggggtatgcc aataccaaca tcagaagaat cgggatcagt ccgccaaaac
    12181 ctgcgcggat agggaagaac agcaccccgg cgccgattgc cgtaccaaac aagcctaacg
    12241 tccatgtggt atctgattta cgccaggacg attgttttgt ctggctggat acaatgctat
    12301 ctgaagtact catatcctat cctcaacgaa ttaattaagc gtcaacgaaa ccggtgattt
    12361 gagagacgcg agaaagatcg atattgccgc cggaaataat actgacggtt tttctatttt
    12421 gaatatattg gtctaattta ccgcttaata atgcagcaca tgccagagcg cctgcgcctt
    12481 cggtgacgac tttattgcgc tgaattaagg caatcatact gtttctgatt tcgtcttcgc
    12541 tgaccagcac gatgtcatcg actaattcac gaacgatttc gtaagttaaa ttacccgggc
    12601 gggagacatc acaaccatcc gccagggtgc cggtagttcg gtgcgtggtt atttctccgg
    12661 agtggaaaga agccgccatg ccgtgaacgt tttctgactg tacgccaata acacgaatgg
    12721 tcgggttaat agatttaatt gccaccgcaa taccagcaat taaaccacca ccaccaattg
    12781 gcacaatcac gttatcgaca tcatagagat cttccataat ttccagacca atcgttccct
    12841 ggccagcaat cactttcgga tcatcgtaag gtgggataaa aatacgacct tccatttcga
    12901 caatttcgct cactttagcg atagtgtcgt tgaagttatc accatgcaga acgacttctg
    12961 cggagtagtc gcacgttgcc gctactttgg attttggcgc accttttggc atcaccactt
    13021 taccgtcgat acccagcatc gcgcaggaga gcgaaacccc ttgcgcatgg ttgcccgcag
    13081 aacaggccac cacgcctttg cgtttttccg catcggtcag tgaacttaat ttattaaatg
    13141 cgccacgaat tttaaatgaa cccgtacgct gcatattttc aaacttcagg aatatttcac
    13201 ctttgcaacg ttcactaaaa tagttggagc gaggcatgcc tgttttataa attcgcccag
    13261 ccagtcgttg tttcgcttca ataatatcat caatagcaac cggcagatcg tatgtaatat
    13321 gcattataaa acctcttcgc ctgaattaaa tatgtaggta accgtaaccg acacctgcaa
    13381 aacagacagg tggattattt atgtttaaat aaaaacaaac aactaaccaa cttctattaa
    13441 ttgccttcgt ctacacccat tataagatga atactctttg gctaattcca ccaaaaccga
    13501 tgctgctttt ttaatacgat aatttttcga ccataccgcg gcatattgtg ccacaggtaa
    13561 tgtttcttca accggaatag taataaattg attagaacca aaaggtgacg tcatatcaca
    13621 aggaattaca gttaagaaat cagcattgag aacaagatta taaattgtca cgactgagtc
    13681 ggttttaacg atgttttcaa tactgatgcc atttctttgt aacgtagtaa gcagttcgct
    13741 gtagtacccc atattagttt gtggcaacac ccactgttcg ttcttcaacg actccagcgt
    13801 ggtggtgccg gtgcatgttc gggacttact ggctaccagc acaaactcgg actcgaacag
    13861 cggctcaaca tgtaaatcct gaagcttcat ttctgcactt aacgtaccaa tcgcaaaatc
    13921 cagtcgaccg tcgcggattg ccggtaagaa cgaagacagt tgcgcttcat acatagaaac
    13981 ctgcgctttc gggaacacct ctttgaactt gttgatcatc cctgacataa aagtaaaacc
    14041 aatcaatgaa ggaaaaccaa atgagacttc caccaccgct tcagaagaca taccgcttat
    14101 ctcattaacc atatttttca tttcacgggt aatggattcg gaacgggaga gtaacaattg
    14161 accggcaggt gttaatgtta caccggtatt tttccgcacc actaattcca caccaaaata
    14221 atcttcaata tcgttaatga ttttactgac ggccggttga gttaacccta attcttttgc
    14281 agccgagccg atagagccac ttctaatgac ttcctgaaag actaccaggt gctgcgtttt
    14341 cggaagaaga atagtgctca taacgaccta cgttaattac ctcattgacg gcatgaagtg
    14401 tatcaaaatg aaataaacag gatatgtgcg accactcaca aattaacttt cattactttc
    14461 caaggtatcg ttattacaat taaacatctt taaatatcaa caagttaaag cataaaaatc
    14521 agcataaacc ctgattttta tcaaaaaaaa atgacgggga taaccatatt ttatggcgat
    14581 aacatcattc gttatgtggt taatacaaaa aaggggctga gaccatattt caagcaatta
    14641 ccggataatt accaggcaaa atttgagtta aatttaatga gcaagttctc aaattttatt
    14701 ataaataaac cattttcagc gataaatact gcggcacgtc acattttttc acgctatttg
    14761 ttggagaaca aacatttatt ttatcaatat tttaaaattt cgaatacagg tattgatcat
    14821 ctcgaacaat tgattaacgt caactttttc tcttctgaca ggacgtcatt ttgtgaatgc
    14881 aatcgttttc cataaattct tctcccctca taggcgacga atagcatttt gtgttgagga
    14941 tcacaaaacg aataattgct ggtcgccgcg ataaggtcag acgaagtcaa caagggggat
    15001 tttcactgtg ctttacatca gcgtaggaca cagaatgttg cttcgtttac tgtaacgccg
    15061 ggtaaatgag cgttttttga tagtgcgtaa gtgtcgtgcg aggaaaataa gcatctatta
    15121 ttgtttgtca tgacgacaag gcaggcatca atgaaaggct tcccaattgc acatattttt
    15181 cacccttcaa tcccgccaat gcacgcagtg gttaacaatc acaatagaaa tattgattat
    15241 tggacggtaa aaagaaagtt tgcagaaatt gtctccacca atgacgttaa taaaatttac
    15301 agtataagta atgaactccg gagagtatta tctgcaatta ctgcattgaa tttctatcaa
    15361 ggcgatgttc cttctgtcat gattccgaat ccaaccggaa aatatgagtc cattcatcat
    15421 agatatttct acaggagaac atgatgatta tatcatacaa acattagatg taggcacttt
    15481 tgcacctttt ggtgaacaat gtacttgctc agccgtcaat aaaaaagagc tggaatgtat
    15541 taaggagacg atttctaagt attgcgcaaa gttcacccaa aaagaagcca tcttaacacc
    15601 ccctaggcac tttaataaaa cctgcattac ttcagattgt tggcaaattc tctttttttc
    15661 acccgatcat tttaataatg atttttattg attatttata aaggataaaa taatgttccc
    15721 tgtttcatca attggtaatg atattagtag tgatttagtc cgtcgaaaaa tgaatgacct
    15781 tcctgaaagc cccataggaa ataacctcga agcactagca ccaggtatag aaaaactaaa
    15841 acagacctct attcaaatgg tcactttact taacgcgtta caaccaagtg gaaaatgcat
    15901 tatcactggt gattttcaaa aagaattagc gtacttacaa aatgtaattc tttataatga
    15961 ctcgtctctt cgtctggatt ttttaggtta taacacccaa attattcaac gatcggacaa
    16021 tacctgcgaa cttaccatta atgaaccgtt aaaaaactag gaaatatcta caggcaatat
    16081 caatgttaat tgcccattaa aagatattta caatgaaatc aggaggttga acgtaatctt
    16141 tagttgtggg actggagata tagttgatct atcctctctg gacttacgta atgtcgattt
    16201 agattattat gatttcacag ataaacatat ggctaatact attctaaatc attttaaatt
    16261 ggattcaaca aattttacta atgccaacat gtttcaggtt aattttgtta gttcaacaca
    16321 aaacgccaca atctcctggg attatttact aaaaataacg cctgttttaa taagcattag
    16381 cgatatgtat tctgaagaaa aaatcaagtt tgtcgaaagt tgtttaaatg agcttggaga
    16441 cattaccgaa gaacaattaa aaattatgag atttgcaatt ataaaatcta taccaagggc
    16501 aactcttaca gataaattag aaaatgaatt aacaaaagaa atatataaaa gctcatcgaa
    16561 aatcaacaat tgcttgaaca gaattaaatt accagagatg aaagaattct catcaaaaaa
    16621 aacatatgat tacatcgata caatcattga agattatgaa aatatcaaag aaaatgctta
    16681 tctggtcgtc ccccaaatta attatactat ggatttaaac atagaagact ctagctcaga
    16741 agagttaata ccctcgagaa agacgaaaat tctccggaca atggctttga ggtcggggaa
    16801 tataacacat atgaagcata taactcagag aatcaatatt ttaccagaga ggactatacg
    16861 tataattacg accttttaaa tgcaatatag acttaataac ttcttacaaa gtctattatt
    16921 aataccgtag atattcatct taccctgtgc accaaaagca aaaacttatc gacgcgggtt
    16981 tgaaactgat ggcaaccgca aaaatgcctg atgcgctacg cttatcaggc ctacgccatc
    17041 tctgcaatat attgaatttg cgtgcttttg taggcaggat aaggcgttca cgcagcatcc
    17101 ggcatgaata aagcgcactc aacaatctga aacccgccgg aacggtttat tacgtacatc
    17161 aggtgaaact gaccgataag ccgctttctt ttgggtatag
//
LOCUS       DAJTSI010000086        17091 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000086 DAJTSI010000000
VERSION     DAJTSI010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17091
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.086"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..510
                     /locus_tag="OO327_002550"
     CDS             <1..510
                     /locus_tag="OO327_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HCR6019051.1"
                     /translation="PFREKPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDG
                     FTQPIVVTHTDKNAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATI
                     RHNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADR
                     QYSRAWTVK"
     gene            complement(511..1671)
                     /gene="ybdL"
                     /locus_tag="OO327_002551"
     CDS             complement(511..1671)
                     /gene="ybdL"
                     /locus_tag="OO327_002551"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HCR6019052.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1780..2868
                     /gene="hcxA"
                     /locus_tag="OO327_002552"
     CDS             1780..2868
                     /gene="hcxA"
                     /locus_tag="OO327_002552"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCR6019053.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(2878..3075)
                     /locus_tag="OO327_002553"
     CDS             complement(2878..3075)
                     /locus_tag="OO327_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR6019054.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3257..5362)
                     /gene="cstA"
                     /locus_tag="OO327_002554"
     CDS             complement(3257..5362)
                     /gene="cstA"
                     /locus_tag="OO327_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCR6019055.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWLKAFSPDAKVGFLVIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5543..5956)
                     /gene="entH"
                     /locus_tag="OO327_002555"
     CDS             complement(5543..5956)
                     /gene="entH"
                     /locus_tag="OO327_002555"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCR6019056.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTLLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(5959..6705)
                     /gene="entA"
                     /locus_tag="OO327_002556"
     CDS             complement(5959..6705)
                     /gene="entA"
                     /locus_tag="OO327_002556"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCR6019057.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALTFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6705..7562)
                     /gene="entB"
                     /locus_tag="OO327_002557"
     CDS             complement(6705..7562)
                     /gene="entB"
                     /locus_tag="OO327_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCR6019058.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7576..9186)
                     /gene="entE"
                     /locus_tag="OO327_002558"
     CDS             complement(7576..9186)
                     /gene="entE"
                     /locus_tag="OO327_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCR6019059.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCSIPAAHNYTMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9196..10371)
                     /gene="entC"
                     /locus_tag="OO327_002559"
                     /pseudo
     CDS             complement(9196..10371)
                     /gene="entC"
                     /locus_tag="OO327_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            10663..11619
                     /gene="fepB"
                     /locus_tag="OO327_002560"
     CDS             10663..11619
                     /gene="fepB"
                     /locus_tag="OO327_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR6019060.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(11623..12855)
                     /gene="entS"
                     /locus_tag="OO327_002561"
     CDS             complement(11623..12855)
                     /gene="entS"
                     /locus_tag="OO327_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCR6019061.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPELPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            12966..13970
                     /gene="fepD"
                     /locus_tag="OO327_002562"
     CDS             12966..13970
                     /gene="fepD"
                     /locus_tag="OO327_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCR6019062.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            13967..14959
                     /gene="fepG"
                     /locus_tag="OO327_002563"
     CDS             13967..14959
                     /gene="fepG"
                     /locus_tag="OO327_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCR6019063.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAAVLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            14956..15771
                     /gene="fepC"
                     /locus_tag="OO327_002564"
     CDS             14956..15771
                     /gene="fepC"
                     /locus_tag="OO327_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6019064.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(15768..16901)
                     /gene="fepE"
                     /locus_tag="OO327_002565"
                     /pseudo
     CDS             complement(15768..16901)
                     /gene="fepE"
                     /locus_tag="OO327_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
BASE COUNT         3994 a         4865 c         4442 g         3790 t
ORIGIN      
        1 ccctttcgtg aaaagccagt ggattgcgtt ctgtgggtta aaaacagcca acttatgcct
       61 aatgattaca acccaaacaa cgtggcacca cccgaaaaaa aactgctgca aaaatccatt
      121 gaaatagatg gttttaccca acccattgtg gtcacacata cggataaaaa tgccatggaa
      181 atcgtggatg gttttcaccg ccatgaaatt ggtaaaggct catcatcatt aaaattacgc
      241 ttaaaaggtt atttgccggt gacctgtctg gaaggtacgc gtaatcaacg catcgccgcg
      301 actattcgtc ataatcgcgc tcgtggacgc catcaaatca ccgccatgtc ggaaatcgtc
      361 cgtgaactta gccagttggg atgggatgat aataaaattg gcaaagagct gggaatggac
      421 agcgacgaag tgctgcgact gaagcaaatt aacggcctgc aagagttgtt tgccgaccgt
      481 caatattccc gcgcctggac ggttaaatag ctaaagctgg cgcaggcgtt cagctgctgc
      541 cagcaacgtc gattccttct tggcaaaaca gagacgaatc agtttatgtg ggaagggatc
      601 ggcgcaaaac accgacagcg gaatcgccgc tacgccgtgc tcctgcgtca gccactggca
      661 aaactcaaca tcatccaggg tagaaaccgc gctgtaatcc accagcaaaa agtatgtacc
      721 ttcacacggt aaaatctcca gccggctttc atttaaagca ttcaccagaa tatcgcgctt
      781 ctggcgataa aagtccggta acgcaagata atgctcaggt tctgcacgta gcatatcagc
      841 aagcgccagc tgtgccgggg tattcaccga aaaggtcaga tactgatgta ccttgcgaat
      901 ttcggcgctg atgggcgctg gcgcaacaca ataacccact ttccagccag tcatatgata
      961 ggtcttgcca aatgaagaaa ccgccactgc ccgctcacgc agctgcggat gcgccagcac
     1021 actggcatgg ccctgttgtg aaaagttgat gtgctcgtag acttcatcgc taatgacaaa
     1081 aatctcgtgc ccggcgatcg cctgccacaa agcggcgaaa tcagcctgct gccagacagt
     1141 tgcactgggg ttatgcggag tgttgaggat caccagtctg gtgcgctcgc ttaataatgc
     1201 ggcaaattcc tgccagtcaa cgcgaaaatg cggtggttgc agtgccatac gtttcactat
     1261 tcccccagaa agcgcgatgg cgggggcgta actgtcatag ctgggatcaa aacaaatcac
     1321 ttcatcgcca ttgcgcacca gtgcggtaat cgccgcgtat aacgcttccg tcgcccctgc
     1381 cgttacggtg atatcgctat cggcatctgg ttgatagcca tacaaacgtt ccgttttctg
     1441 agcaatcgcc tcgcgcaagg cctgcacgcc ggtcatgggc gcgtattggt ttgccccctg
     1501 ggcaacgtgg tacgccagcc gctcctgtaa atagcgcgga ccatcaaaat caggaaagcc
     1561 ttgcgacagg ttaatcgcct ggtgttgctg cgccagcgcg ctcatctggg tgaaaatagt
     1621 ggtgccaagt tgtggaagtt tgctttgtgg aatcagaggg ttatttgtca tttttattgt
     1681 acctgacgaa gttgcgtgtt gaaggcacta taacacgatg ccagtatttg gcaatcaaga
     1741 cgtttagatg tctaaataaa acaataagga caacacaaca tgcctcacaa tcctatccgc
     1801 gtggtcgtcg gcccggctaa ctacttttca catccaggaa gtttcaatca cctgcacaat
     1861 tttttcactg atgaacaact ttctcgcgcg gtgtggatct acggcaaacg cgccattgct
     1921 gcggcgcaaa ccaaacttcc gccagcgttt ggacttccag gggcaaagca tattttgttt
     1981 cgtggtcatt gcagcgaaag cgatgtacag caactggcgg ctgagtccgg tgacgatcgc
     2041 agcgtggtga ttggcgtcgg cggcggcgca ctgctcgaca ccgcgaaagc cctcgcccgc
     2101 cgtctcggtc tgccatttgt tgccgctccg acgatcgccg ccacctgcgc agcctggaca
     2161 ccgctctccg tctggtataa cgatgccgga caggcgctgc attatgagat tttcgacgac
     2221 gccaatttta tggtgctggt ggaaccggag attatcctca atgcaccgca acaatatctg
     2281 ctggcgggga tcggtgacac gctggcgaaa tggtatgaag cggtggtgct ggctccgcaa
     2341 ccagaaacgt tgccgctaac cgtgcggctg gggatcaata atgcgcaggc cattcgcgac
     2401 gtcttgttaa acagtagcga acaggcactt gccgatcagc aaaatcagca gttaacgcaa
     2461 tcattttgcg atgtggtgga tgctattatt gctggtggtg ggatggtcgg tggtctgggc
     2521 gatcgtttta cgcgtgtggc ggcggctcat gccgtgcata acggtctgac tgtcctgccg
     2581 caaaccgaga agtttcttca cggcaccaaa gtcgcttacg gaattctggt gcaaagcgcc
     2641 ttgctgggtc aggatgatgt gctggcgcaa ttaaccgggg cgtatcagcg ttttcatctg
     2701 ccgactacgc tggcggagct ggaggtggat atcaataatc aggcggagat cgacaaagtg
     2761 attgcccaca ccttgcgccc ggtggagtcc attcattacc tgccagtcac gctgacacca
     2821 gatacgttgc gtgcagcgtt cgaaaaagtg gaatcgttta aagcctgacg tgcaaattta
     2881 gcagcagcgc gcgccgcctt ttccaccgta gcgcgcgtcc tgccgctcac ggaaaaactc
     2941 ctcgtaggtc atcggcgttt gatcgggatg attaacccgc atatgttcga catagttgtc
     3001 gtaatcaggc ataccaatca tcagcttcgc cgcctgacct aaatattttc cggcttttgc
     3061 cagtgaatca aacatcttgg cctccgctaa ccatgttgct gaaagagtgc cggatgcggc
     3121 gtgaacgctt tatccggcct acaaatgcta tcaacgcact cggtcagtcc cctcgcccca
     3181 tcggggagag ggttagggtg agggggaacc ctcggcacta tgccaacatt cgccaacatc
     3241 ccccctcact ctgactttag tgcgcgcctt tcgcctgcgc tacgatctcc tcgacatttt
     3301 ccggcatcgg ctcatacggc gtttctttcg ccgttggctt cggatctttc aatgccgcaa
     3361 gtgccgtctt aatcgagaac agtgccagga ccaccacgac caccataaag aagatggtta
     3421 acccggcatc cagacggttg ttgaacacca gttgcgccag ctgtgactca gtatactgcg
     3481 acggaatatt gccgctgtcg atcattgcct ggaacttgtt agcaatgacc aggaagccga
     3541 ctttcgcatc cgggctaaac gctttcagcc agtcagcggt cagggtacaa atcagcagcc
     3601 aggccgttgg taccagcgcc acccaggcgt aacgttgacg tttcatcttg aacaacacca
     3661 cggcacagag catcagcgcc atccctgcca gcatctggtt ggcaatacca aacagcggcc
     3721 acagagtgtt aataccgccc agcggatcga ccacgctctg atggaggaag tagccccacg
     3781 ccagtacgca cagcgctgtt gccagcaggt tagcaggcag tgaatcggtc cgcttcaggc
     3841 caggagacac cacgcccagc agatcctgca acataaagcg cgcagcacgc gtacccgcat
     3901 ctaccgccgt cagaataaac agagcttcaa acagaatggc gaagtgatac cagaacgcca
     3961 catccatcat accacccagc gcaccgtgca ggatgtaagc catccccacc gccaacgtcg
     4021 gcgcaccgcc cgcacgggaa atgatcgact gttcacccac ttcgctggca atctggttga
     4081 gcgtatctgg tgtaatgcta aagccccagc tactcaccac ctgcgcggca gaagcgacta
     4141 catccgccgt ccctgccgga gccagcaccg ccattgggct gttcatggca aaatacacgc
     4201 ccggatcgat gatacaggca gaaactagcg ccataatcgc cacgaaggat tccatcaaca
     4261 tcccaccgta gccgataaag cacgcctgcc cttcgttcgc cagcatcttc ggcgtggtgc
     4321 cggaagagat cagcgcatgg aagccagaca ccgcaccaca ggcgatggtg ataaacagga
     4381 acgggaacag gttaccggtc cataccgggc cagtgccatc gacaaatttg gtcagcgcag
     4441 gcatggtcag cgtcgggcgc ataatcaaaa tgcctaccgc cagaccgacg atagtcccga
     4501 ttttcaggaa ggtcgagaga tagtcacgcg gggccagcag caaccacacc ggcagcaccg
     4561 ccgccacaaa accgtagccc accagcatcc aggtcagctg cacgccggta aagtcaaagt
     4621 acggtgccca ggtcggactt tctgccaccc agccgccaga gataatggcg aaaatcagga
     4681 acaccaggcc aatcactgac acttcgccaa tgcggcccgg acgcagatag cgcaggtaga
     4741 ttcccataaa aatcgccagc ggaatggtga acgcaacggt atacgttccc cacgggctat
     4801 gagtcagggc tttcaccacg atcatcgcca gtactgcaag gataatgacc atgatcataa
     4861 agcaggctac cagcgcaatc accccggcag ttggccccat ctcttctttg accagctcac
     4921 ccagcgagcg accgtcacgg cgcgtagaaa caaacagcac catgaaatcc tgcaccgcac
     4981 cggcgagaac caccccggcg agcagccaga tcatccccgg caggtagccc atttgcgctg
     5041 ccagcaccgg ccccaccagc ggccctgctc cggcaatggc cgcaaaatgg tgaccgaaca
     5101 gcactttctt gtccgtcggc acatagtcca gcccgtcgtt atggcgcacc gctggcgtca
     5161 tacgcgtcgg gtcaaccgcc agcacatttt tggcgatata cagcccataa aaacggtaag
     5221 cgatcagata gatacagacc gacgccacca caatccacag cgcgttgatc tgttccccac
     5281 gatttaaagc aatgtatccc agagcaaatg ctcccattac agagagcact gtccagacga
     5341 ggtatttccc tgatttgttc atagttgtta tccgtgtgcg tgtccataga gatgttacat
     5401 tttgtttcta taacatcttt gtacgattta acaacacggt aaccatgtaa atcggagttg
     5461 aaaccaatat ttaacttaac aatgttaact cgatcactcc gttaaccgag ttttcctgca
     5521 atctcattta attctgtcgg gctcatccca aaactgccgt acccagccga caagtgcagc
     5581 aacgccgccc ctgttcatcg aaaacgacga tttcccaact ctgattttgc ctcccaagat
     5641 gcagcggctg gcagacgccg cgtaccttgc cttcagacac cgggcgatga tgggtggcgt
     5701 taagttccgt gcccaccaca cactgcccgt cacgggtcat cataaatccg gccatcgatc
     5761 ccagcgtttc agccagcgcc gccgacgcgc cgccatgcag caggccaaac ggctgatgag
     5821 tacgggtatc aaccggcatt tcggcttcca gcacatcatc tcccagaagg gtatacacaa
     5881 ttcccagatg cgccaccatt gtgttatcgc tggtggcgtt cagttcgtcg agcgttaaat
     5941 ggcgtttcca gatcatgctt atgcccccag cgttgagccg ccatcgacca caatatcctg
     6001 tagggtgata tggctggcga ggtcagaggc gaggaacaaa atcgtgttgg cgatctcttg
     6061 tggacgggcg attttcccca gcggaatgcc gagtttaaac tgctcgccaa agccgcgaat
     6121 acgctgttct tcggcgtcat cgctcaccca cagcgtgcgt tgcatatcgg tgtcggtgga
     6181 gccaggcgaa accacattac agcgcacgcc gctacccgcc agttccagcc cgacgctcaa
     6241 cgccaggctt ttcagcgccg ctttcgatgc gccataagca ctcataccaa tacgcgccgt
     6301 gtgtgcggcg tcggacgcca cagtgacaat cgccccgccc cgctgacggc gaaactggtt
     6361 catggtttgc tggaacaggt taaacgcacc gccgacgtta accgcaaaag tctgctgcca
     6421 gtcctcttta ctgagctgat cggtcgcgcc catgcgcaaa attcccgccg cattgatcag
     6481 cacgtccagt cgctccgttt cagctaacag tcgctgacat acctgcgcca cctgcccggc
     6541 gtcggcaaca tccattactt cggtcgcaaa gggatattgc tcctgagtga acgcttgatc
     6601 aaaacctgta actttcgctc ccgcctcaac aaacgtcagc gccgtggcgt agccgatacc
     6661 tttacctgcg ccggttaccc agacattttt accgctgaaa tccattattt cacctcgcgg
     6721 gagagtagct tccaccaggc gtcgatggtc gggtttttcg ccagcatgac aaagtcgata
     6781 tcaccatgca ctttgcgcca gcgcgccgcc agcgccatca tgcgcaccga atccagaccg
     6841 tagtcgatca ggttgtcgtc atcgaacggt tcatcggact cgtccagcaa cggcaggatc
     6901 acctcacgca gcgccgcttt gctggcgggg ataggtgctg gcagtaattc ttcggtcatc
     6961 accacccggc cagaacgtcc ggccacatat ttcagcgaca tcaaatgctc gtcacggctg
     7021 aaatcagcca gcgcatccgc caccataaac ggtttaatat cgcgcataaa tgcgtcggtt
     7081 gcggtggtca tacagccaat gtgggcatat actcctgtaa taatcagctg gttacgtcca
     7141 ctctctttga gcatttgctc cagcggagaa cgatgaaacg cgctgtagcg ccacttcacc
     7201 agcaccgtgt cgtcggcatc tggcgtcagg cgatccacca ccttttgctg ttccggcgag
     7261 cgggtcaggc ccggccccca catatcattc aacagcgccc gatcttcatc gctctgctct
     7321 ttcggctggg cggtgtaata aaccgggata ttgtgctgtt tgcagtagtc gcgcagcgca
     7381 gcaatattcg cgatcacctg ctccatcatc gggcagttct cgccccagaa gctgacaaaa
     7441 tagtcctgca tatcatggat taacaacgcg gcacgttgcg gttcaaaggc ccagtcaact
     7501 ttattctgcg gaatatcgtg agactccggc agtgcgtaag cctgtaattt tggaatagcc
     7561 atcgtgttct ctccttcagg ctgatgcgcg tgacgccagc cactgacgta attgtttttt
     7621 atcgactttc ccgaccgccg tcagcggaag tgaatccaca cactccacgc gatccggtaa
     7681 tttaaattcg gcaataccct gttcacgcag gaaacgacgc acctgcaccg cgcgcagcgg
     7741 ctcttttacc accagataag cacagctttt ttcgcccatc agctcatttt ccatgctcac
     7801 cagtgcggca tagatcactg ccggatggct cagcagcagg ttttcgatat cttcggcagc
     7861 gatcttctcg ccgccacggt tgatctgatc tttctcgcgc ccctgcacgg tgatgtaacc
     7921 ctctggatca atagagatca gatcgccgga acagtaaaaa ccgttggcat caaaggcact
     7981 ggcattgtgc tgcggacttt tgtaatagcc acggaaggtg tacggcccgc gcgtcatcag
     8041 gcgtccaact tccccttgcg gcagtggatt tccttcggca tcggcaaccc acacttcatc
     8101 gtccggacac attgggtaac cctgggtatg gataattttc tccgcgctat catcaagacg
     8161 ggtgtagttc accagccctt ccgccatgcc aaacacctgc tgcaactggc agccaatctc
     8221 agcgggaata cgcgctgcaa gcgtggcaga aagacgtgcg ccgccgacct gtaacagttt
     8281 cagcgaggca agctgcgccc ggctttcgcc ttcagccagc gcctgcaacc acaggctgac
     8341 tgccggcggc accagcgcgg taacgttaac ctgatgtttt gcaatcaacg ggaagcaaag
     8401 cgtggcgctg ggatcggccg ccagaacaac cgttcctccg gcaagaaaga cgcccagcga
     8461 tcctggcgaa ctcatagtgt agttatgagc cgccgggatc gagcacaggt agcgcgtctg
     8521 ttgtgtgaac tgacaaatct cggcgctacg acgcacgctg tagtaatagt cgttatgagt
     8581 gcgcgggatc agtttcggtg tgccggtggt gccgccggaa agctggaaat aggccacttc
     8641 atcagcaggt gatggcgtgg cagtaaaacc ctctgccgga tggttaatcg catcctgcaa
     8701 gttatgctca ccgctgtcgt tgtgcagttg caccacgcga atggaagaat gctctgcaac
     8761 aaatgtgttg aggaaatcat ccccgctaaa tagcgcatgc tggcgatcgg caatcagtaa
     8821 cgccggttca atctggctgg cgtaggcgtt cagttcacta cgctgatggc tgaacagcgc
     8881 cagcaccggc gcgacgccca gtttcagcag cgcgaaaaag gtaatgtaaa gttcagcgac
     8941 gttacccagt tgtaccagcg cggtttcacc aggtttaatg ccctgacggc gcaaactaca
     9001 ggcgaggtta tccgccgcct gattcagctc ccgataactc aactgccgct cgccgtcgat
     9061 aaccgcgatg ctgtcactcg cagcgtggcg agtcagaatg tcggtcagtg gcaaatcctg
     9121 ccagtagcct ttttcccgat agcgacgggc aaactcttcc ggccagcggg tgaatggaat
     9181 gctcatcctc gctccttaat gcaatccaaa aacgttcaac atggtagaaa gtttgacgcc
     9241 tgtttcgcgc cactcaccca acggtgacga cgcaggcaca atccccgctc cggcaaacag
     9301 acgcacctga ttttcccgca gcttcgcgca gcggatggtc accacccatt cgccgttacc
     9361 ttcgctgtca caccaaccca caatgccgcc aaacagttcg cggtcgaacg gctccagttc
     9421 agcaataacc tgggtcgcgg cctgatgcgg gaaaccgctc agcgcggggg tcggatgcag
     9481 cagacaggcc agagtcagtg cgttttcttg cgaattcgct ttaccttcaa agggagttgc
     9541 gagatgccac agcgtcggcg tggtaatcaa ttgtggagag gagggaacgt gtaactcact
     9601 actgcgttcg cgcagtacct ctttcatcgc ctgagtcccc agttcatgtt catggcgatc
     9661 tttttctgac gccagcagac gattacccgc ttcgcgatcg agcacttcat ccggctgacg
     9721 acgcgcggaa ccggctaacg gaatggagct aaaacgctcg ccgtctttac gtagcagcag
     9781 ttccgggctg gcccccagca ggacgccacc atcagccagc gggacatgga agttgtaact
     9841 aaccgggttt tgcgcaatca accgttccag caatacgcca ctatcaatgg cggcgtcagt
     9901 ggtgatatca atcaatcgtg acaacaccac tttgtcgacc tgcggcgtgg cggtaagtgc
     9961 ggcggcgcgg gcacccatct gttcaaacgt ggtttgttca ggaattgcct agcgttccac
    10021 cacgttcagc gactggctgc gggtaaaaca gcgggctgag gtctgttttt cctgacggga
    10081 gaacgactgc taggattcgg gaatatacag cgacgaaggc tgacgtggat cgaagggaat
    10141 cgccccgacc atcaccggat ttttgatgcc ctgcgctttg gcatcggcaa acagcgcggc
    10201 gagtttttgc tggaagggac tgtcgggcga atccccgttc acagccggtt catcgaaacg
    10261 ggcgaaacat cctgacgtcg taaaactgcg gtacggcgac ataaagaaaa agcgattggg
    10321 cgcaagtgtt gccatggtct gctgtacttc ctcagccagt gacgtatcca tatcatcctc
    10381 cacaaaatga taaaggcttt aataatgatt atcacttata ttttcggccg ctaacctaaa
    10441 agcaaacgcg cactatgtca actcttgagg taacgcaatc tgcaactcgg ggttgcatct
    10501 ggttgtggtt acaatgaaaa tgagaagcat tattgatgaa ttcgcataag cgcaatgtga
    10561 tgtcctgcgc cgttttgccc cctctcccca aagggacgga ttgtgcgctt tgtcgaattt
    10621 gtcattacgc ccttaacctt attaataaca ggaagctgac ttgtgagact cgccccgctc
    10681 taccgcaacg cccttctatt aacaggactt ttgctttcag gaatagccgt agttcaggcc
    10741 gccgactggc cgcgtcagat tactgacagc cggggcactc ataccctgga aagccagccg
    10801 cagcgtattg tttccaccag cgtcaccctg accggatcac tgctggcgat tgatgctccg
    10861 gtgatcgcca gcggcgcgac cacgccgaat aaccgcgtcg cagatgacca gggcttttta
    10921 cgccagtgga gcaaggtggc gaaagaacgc aaactgcaac ggctctatat cggcgaaccg
    10981 agcgccgaag ccgttgccgc gcaaatgccg gatctgattt taattagcgc aaccggcggg
    11041 gattcggcgc tggcgctgta tgatcagctt tccaccatcg ccccgacatt aatcatcaat
    11101 tacgacgaca aaagctggca gtcgctgtta acgcagcttg gcgaaattac cgggcatgag
    11161 aaacaagcgg cagagcggat tgcacagttt gataagcaac tggcagcggc gaaagagcaa
    11221 atcaaattac cgccgcagcc ggtcactgcc attgtctata ccgccgctgc acacagtgcc
    11281 aatctctgga cgccggaatc agcacaaggg cagatgctgg aacaactcgg ctttacgctg
    11341 gcgaaattgc ccgcagggtt aaacgccagc caaagccagg gcaaacgcca tgacatcatt
    11401 cagcttggtg gggaaaatct ggctgcgggg ttaaacggtg aatcgctatt cctgttcgct
    11461 ggtgatcaga aagatgccga tgcgatttat gccaatccac tgctcgcgca cctgcctgcg
    11521 gtacaaaaca agcaggttta tgcgctggga accgagacgt tccgcctgga ttactacagc
    11581 gccatgcaag tgctggaacg gcttaaggcg ctgttttaag cattaactgt cggacgctgt
    11641 cacctgcggc ggcgtctggc gaaaacgtcg caactccacc agcaccagca ataacaacac
    11701 gccgataatc aacaaaccaa aaccgctcgc gcttgcggaa gcaaccggtg tcatcatcgc
    11761 gcccaaacca cccagcagcg ccgcgcctat ggcatcgccc gtcacgttct gcgccgtcca
    11821 caaaccgtta atccgcccta acatcgcttc cggggtttgc gtttgcagca ttgtatattg
    11881 cagcaacgaa ctaaccgcgc tcagccagcc gaacagcgcc agacaaacca cgcctaaaat
    11941 ccacatcggc atcaggccaa acagaccaat ggcgaggaac gatcccagcg tggagagcag
    12001 catcaataac cctggtcgcg cactatgtgc cagcttcccg ctggttaacg caccaatagc
    12061 cgcgccgagc gggatcgccg cgtagagaaa accaatctgt gccgctgaca tctgccagtt
    12121 gtcagccagc gccggataca gtaccagcac cgcgctcgcc atcgtcaata aaccacccag
    12181 cagcgggctg gcaagcagaa aacgaaatcc tgccagtaat gatttcaacg gatgctcaag
    12241 cggctgcggt ggcggtggca actccggaag gcttaataac ggtaacaagg taataaacgt
    12301 gcccgccgcc gccagcccgt agttccaggc tacgccgccg gtcgccagca ataaaccgcc
    12361 aatcatgggc gaaatcaccg accccaggcg cacggtcaac atggtgatcg ccccggcctg
    12421 cattaagttt tcacgcccta ccagtgcaga tgttgccgcc agtagcgccg taacaccaag
    12481 tgatgcgaaa aaaccatccc ataaaccaag taaatagatt gccagcaatg acggctccgg
    12541 cagcagtgca ttaaggcaca gtccaatgaa gccaatgcca caggtgccgc gcgccagcaa
    12601 aatcactttt ttgcgttcat agcgatccgc cagcacaccg ccaaccatca ggccaacaaa
    12661 catcgcgccg ccggtcagcg tcaccgaaag ccccacctgc caggtagaat gggtcatcat
    12721 ctggatctgc accggcaccg cgacgccgag caaacccaga gacacaattg agataaaacg
    12781 agcgaggaat actgcgcgaa acgccgggtg cgttttcaac aggctgaggt taagcagcca
    12841 ggattgttta ttcattacaa tgccttgcca tcaaatattt taaataccta ttcttaggct
    12901 gcacatgtta acatatccaa ataagatcga taacgataat taatttcatt atcatggaag
    12961 ttcgtatatc tggttctgtt gccgtgacac gcgccattgc cgtgcccgga ttgctgttat
    13021 tactgattat cgcgacggca ttaagcctgc tcattggggc aaaatcactc cccgcttccg
    13081 tagtgctgga ggccttctcc ggcacctgcc agagcgccga ctgcaccatc gtgctcgacg
    13141 cgcggctgcc gcgtaccctt gccggtttac tggcaggcgg cgcgcttggc cttgccgggg
    13201 cgttaatgca aaccctcacc cgaaacccac ttgccgaccc cggcttgctt ggcgtgaacg
    13261 ccggagccag ctttgccatt gtgctgggtg cggcgctgtt tggttactca tccgcacagg
    13321 aacaactggc gatggccttc gccggggcgc tggtggcctc attgattgtt gcctttaccg
    13381 gcagtcaggg tggagggcag ttaagtccgg tgcgtttaac cctggcgggc gtggcgctgg
    13441 cggcggtgct ggaaggactg accagcggca tcgccctgct taatcctgac gtctacgatc
    13501 aactgcgttt ctggcaagcc ggttcgctgg atattcgcaa tctacatacc ttaaaagtgg
    13561 tgctgatccc ggttctgatc gccggagcaa ctgcgctatt actgagtcgc gcgctgaaca
    13621 gtttgagcct cggcagtgac accgcgacgg cgctgggcag tcgcgtggcg cgcacacagt
    13681 tgattggtct gctggcgatt accgtgcttt gtggtagtgc gacggcggta gttggcccga
    13741 ttgcctttat cggcctgatg atgccgcaca tggcgcgctg gctggtaggt gccgatcatc
    13801 gctggtcgct gcccgtcacg ctacttgcta cccctgccct gctgctgttt gccgatatca
    13861 tcgggcgggt gattgtgccc ggcgaactgc gcgtttctgt ggtcagtgcg tttattggtg
    13921 caccggtgct gatcttcctc gtgcgacgta aaacgcgagg tggtgcatga tttacgtctc
    13981 tcgccgatta ctcatcacct gtttgctgct gatttccgcc tgtgtggttg caggtatctg
    14041 gggattacgc agcggtgccg tcacgctgga aatctcgcag gtattcgccg cgttgatggg
    14101 cgatgcgccg cgcagtatga cgatggtggt caccgaatgg cgtttaccac gcgtgctgat
    14161 ggcgctgttg attggcgcgg cgctgggcgt cagcggcgcg atttttcagt cgctgatgcg
    14221 taacccgctc ggcagccctg acgtaatggg ctttaacacc ggggcgtgga gcggcgtgct
    14281 ggtggcgatg gtgctgtttg gtcaggatct gacggctatc gcgctggcag caatggtggg
    14341 cggcattatc acttcgctgt tggtctggct gctcgcctgg cgtaacggta tcgacacctt
    14401 tcggttgatt attatcggta tcggcgttcg cgccatgctg gtggccttta atacctggct
    14461 gttgctgaaa gcgtctttag aaacggcgct aacagcaggt ttgtggaatg ccggatcgct
    14521 caacggcctg acgtgggcaa aaacctcgcc ttccgcaccc atcattatat tgatgctcat
    14581 tgccgccacc ttgctggtgc ggcgaatgcg cttgctggag atgggcgatg ataccgcctg
    14641 cgcgctgggc gtcagcgtcg agcgttcgcg tctgttaatg atgctggttg cagtggtgct
    14701 taccgctgcg gcaacggcgc ttgccgggcc gatttccttt attgctttag tcgcgccgca
    14761 cattgcccga cgcattagcg gcaccgctcg ctgggggctg acccaggcgg cgctgtgcgg
    14821 agcgctgtta ctgctggcgg ccgttctctg cgcccaacaa ctgtttatgc cgtatcaact
    14881 tccggttggc gtcgttaccg tcagcctcgg cggtatttac cttatcgtct tgttaattca
    14941 ggagtctcgc aaaaaatgac cgaatcagta gcccgtttgc gcggcgaaca gttaaccctg
    15001 ggatatggca aatataccgt tgcggaaaat ctgactgtag aaattcctga tggtcacttc
    15061 acggcaatta tcggaccaaa tggctgtggt aaatccacgt tactgcgtac cttaagccgc
    15121 ctgatgacgc ctgctcacgg gcatgtctgg ctggatggcg agcacattca acattacgcc
    15181 agtaaagaag ttgcacgccg gattggtctg ttggcgcaaa atgctaccac gccgggcgat
    15241 atcaccgtgc aggagctggt ggcgcgtgga cgttatccgc atcaaccgct gtttacccgc
    15301 tggcgcaaag aggatgaaga agcggtaacg aaagcgatgc aggccacggg aataactcat
    15361 ctggcagatc aaagcgtgga taccctttct ggcggacaac gccagcgagc gtggatcgcg
    15421 atggtgctgg cccaggaaac ggcaattatg ctgctcgacg aacccacgac ctggctggat
    15481 atcagtcatc agattgatct actggaactg ttaagcgaac tgaaccgcga gaaaggctat
    15541 accctggcgg cggtgctgca cgatcttaat caggcctgtc gttacgccag ccatttgatt
    15601 gcattgcggg aagggaaaat tgttgctcag ggagcgccga aggagattgt cactgctgaa
    15661 ctgattgagc gcatttatgg tctgcgatgc atgatcattg acgatccggt agccggaatg
    15721 ccgcttgtgg tgccgctcgg acgaacggca ccttcaaccg caaatagtta aactaagtgg
    15781 tctgccatca tggcatcctg ttttctggat gccatcgcat agcgcaataa cacgctacca
    15841 caagccacca tcccgccgat cagcgcggaa aggatcacaa taatcgcctt acccggaccg
    15901 tcttttttca ctggcaatga cgggcttaac tgatatttaa acggcgtaaa attcacatcg
    15961 ttgacatttg cttttgttaa ttgctcgaca agatactgcc gattacgtaa ttcaccgttc
    16021 agttccgcaa cgtcagtaac cgctttttca atttccagtt tgcgttcaat accgtctgcg
    16081 ccgagggaaa tagagaaatc ggggtcatct ttaacggcct gaccattact gtatacgggc
    16141 tttttaattc ctgccgcgtt ggcaatgtcg agtgaataat tgaggcgctg aatgtttgca
    16201 tcaagttgat ttttcgtttt aatgcgatcc tgagccagtt tttctttttc aaactgggtt
    16261 ttgatctcca gtttattacg gacgttttct atcgactctt tcaccaccaa cgcagagata
    16321 taatcgatat acccgctcaa aacggtctgc gcctctttac tggttggcgc ggtaaaactt
    16381 agcgtccagg atgtatacag tgacggttca tcttttttct tactagcatt gtcatcaacc
    16441 gctttcattt tttcgctcaa tgcaacaata gcgcgatgca aatccagttc atcgattttc
    16501 gcctctttta attagtccat cacataaggt gatgaacgca ggtactcttc cagcaagcta
    16561 acagactgaa acttcttgat aaacaggtta aatgcaccgc cacggtcgat attaacatcg
    16621 agatcaagaa cgcggagttt ggtaaaggtt ttctccaggt cctgccactg aatggcttcg
    16681 gcgggagtaa taacggctga gctggtccat ttttgcggca ggatgaaaga gatcagtaaa
    16741 cctgcgcagg caaatgcaaa aacgaccgcc atgaccgttt ttttggcccg ccataaaact
    16801 tcgattagat taagtaaatc aatttcatta ttactgggcg acgccagagg atactcagaa
    16861 aaatgagcgt cacttccctg tttaatattc aatgatgaca tggggtataa cctgaaaatg
    16921 agtccaatag gggaaaatct ccacggcgct cactttacca gttggtgaga attatccgaa
    16981 gctgaagttt gtaaattcct tccactgaat gcaacattca ctctatttaa gagaaatatt
    17041 catcaaacct ataaatcaaa caaataaaaa acccataatt attttctgta a
//
LOCUS       DAJTSI010000087        16738 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000087 DAJTSI010000000
VERSION     DAJTSI010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.087"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            297..509
                     /gene="cspH"
                     /locus_tag="OO327_002566"
     CDS             297..509
                     /gene="cspH"
                     /locus_tag="OO327_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCR6019065.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            951..1256
                     /locus_tag="OO327_002567"
     CDS             951..1256
                     /locus_tag="OO327_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019066.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            1363..2007
                     /locus_tag="OO327_002568"
     CDS             1363..2007
                     /locus_tag="OO327_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR6019067.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            2004..2750
                     /locus_tag="OO327_002569"
     CDS             2004..2750
                     /locus_tag="OO327_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR6019068.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            2750..4846
                     /locus_tag="OO327_002570"
     CDS             2750..4846
                     /locus_tag="OO327_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR6019069.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            4892..6031
                     /locus_tag="OO327_002571"
     CDS             4892..6031
                     /locus_tag="OO327_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR6019070.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            6019..6465
                     /gene="etp"
                     /locus_tag="OO327_002572"
                     /gene_synonym="yccY"
     CDS             6019..6465
                     /gene="etp"
                     /locus_tag="OO327_002572"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR6019071.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            6485..8651
                     /gene="etk"
                     /locus_tag="OO327_002573"
                     /pseudo
     CDS             6485..8651
                     /gene="etk"
                     /locus_tag="OO327_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(8766..10064)
                     /gene="appA"
                     /locus_tag="OO327_002574"
     CDS             complement(8766..10064)
                     /gene="appA"
                     /locus_tag="OO327_002574"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR6019072.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(10144..10236)
                     /gene="cbdX"
                     /locus_tag="OO327_002575"
     CDS             complement(10144..10236)
                     /gene="cbdX"
                     /locus_tag="OO327_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR6019073.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(10248..11384)
                     /gene="appB"
                     /locus_tag="OO327_002576"
     CDS             complement(10248..11384)
                     /gene="appB"
                     /locus_tag="OO327_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6019074.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            complement(11396..12940)
                     /gene="appC"
                     /locus_tag="OO327_002577"
     CDS             complement(11396..12940)
                     /gene="appC"
                     /locus_tag="OO327_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR6019075.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(13074..13931)
                     /locus_tag="OO327_002578"
     CDS             complement(13074..13931)
                     /locus_tag="OO327_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR6019076.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13928..14326)
                     /gene="hyaE"
                     /locus_tag="OO327_002579"
     CDS             complement(13928..14326)
                     /gene="hyaE"
                     /locus_tag="OO327_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR6019077.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            complement(14323..14910)
                     /locus_tag="OO327_002580"
     CDS             complement(14323..14910)
                     /locus_tag="OO327_002580"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR6019078.1"
                     /translation="MSEQHVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            complement(14907..15614)
                     /gene="hyaC"
                     /locus_tag="OO327_002581"
     CDS             complement(14907..15614)
                     /gene="hyaC"
                     /locus_tag="OO327_002581"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCR6019079.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            complement(15633..>16738)
                     /locus_tag="OO327_002582"
     CDS             complement(15633..>16738)
                     /locus_tag="OO327_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HCR6019080.1"
                     /translation="NWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNV
                     MIPDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFN
                     NVLPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLN
                     EQERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQST
                     LGRILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTE
                     APRGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPL
                     EILRTLHSFDPCLACSTHVLGDDGSELISVQVR"
BASE COUNT         4203 a         4427 c         4233 g         3875 t
ORIGIN      
        1 ttattagaca tattttactt ccttcatttt gggcctttcg gcaaacaggg ctttaataca
       61 gaaaccactt agtgttcagg agagagactc aaaagaaggg gataatgcct gataatgaga
      121 actgctttag taaactactt tgtatgctgt ctgtctttca aaccgacgca gctattaagg
      181 catgataatt gagattaagc aaattttatt ttagccgtcc ggcggtctta aatatcgctg
      241 gctttatttt attaaccccg tatagtgcag cgcagtattt tcagtaagga atttgtttgt
      301 cccgtaaaat gacaggaatt gtcaaaacct ttgatcgcaa aagcggcaaa ggattcatta
      361 tcccctccga cggtcgtaaa gaagtccagg tccatatttc cgcattcact ccccgcgacg
      421 cagaagtgct tataccagga ttacgcgtgg aattttgccg cgtaaatggc ctgcgaggac
      481 caacagcggc aaatgtttat ctctcttaat atgacgctgc ttgtttaaag ctgactggtt
      541 ttattggaat agttcaatag tcaacaccat acattataac atcagttact tttccactaa
      601 gaaactaatc catttaagcg aatatctctg cacctaatag ttataaatgc catttaacag
      661 ccagttatag taccacttga aactatatta ccaacaataa ttaatagctt tcgtaacaat
      721 tttcttatct ttaattcgta tttttctaag atttatctta tgctcacaag tcgtatttcc
      781 agagggaaat cgttaacaat cttatatttc cctctgcgcg cctttcccac ccgcagtata
      841 tagtgctagc ggtatgagga aagggagatt tcacaaggat gttatactca aagtagacaa
      901 tcctgatctc aagcgtacgt attgtccatg cgatgaataa aggaaaagtt atgaaacaca
      961 agctttctgc aatccttatg gccttcatgt tgacgacgcc agctgcgttt gctgcccctg
     1021 aagcagcaaa cgggaccgag gcaacgacgg gtactactgg caccacaacg accactacag
     1081 gtgcaaccac gactgcggct accactagtg gcgtggctgc tggcgctgtc ggcacggcga
     1141 cagttgtcgg tgtggcaacg gctgttggtg tggcgacgtt ggcagtagtt gcagcaaatg
     1201 acagtggcga tggcggttct cataacacct caaccaccac gtccactacg cgctaattct
     1261 gcttaaacgc gtcgaaattc aagttaaata atacccggtt gcgatattcg taccgggctt
     1321 tagagatttg cattttttta attaaccttt caaggattaa aggtgcgccc tcttatttta
     1381 tcgattttcg cactatttct tgcgggatgt acgcacagcc agcaaagtat ggtcgataca
     1441 tttcgcgcca gccttttcga taatcaggat atcactgtag cggctcagca gatccaggcg
     1501 ttgccttatt ccacgatgta tttacgcctt aatgaagggc aacgaatctt tgtggtactg
     1561 ggatatatag aacaagaaca aagcaaatgg ttatcccagg ataacgccat gctggttacc
     1621 cacaatggac gtcttttaaa gaccgtcaaa cttaataata atctgctgga agtgacaaat
     1681 cccgggcggg cccctctgcg taacgcgctg gcaataaaag atggcagccg ctggacgcgc
     1741 gatattctct ggagtgaaga caaccatttt cgctctgcga ccctaagttc tactttttcc
     1801 tttgctggat tagagacgct gaatattgcg ggtcacaatg tgctgtgtaa tgtctggcag
     1861 gaagaggtga cttccacgct gccagaaaaa cagtggcaaa acacattctg ggtcgattcg
     1921 gctactggcc aggttcgtca aagtcggcaa atgttaggcg caggggttat tcccgtagaa
     1981 atgacgtttc ttaaacccgc accatgaata aattacagtc gtatttcatt gccagcgtac
     2041 tttacgtaat gacaccccat gcctttgcgc aaggaacggt gactatttat ctgcctagcg
     2101 aacaacagac actttccgtt ggccccgtgg aaaatgttgt ccagttggtg acacaaccgc
     2161 aactgcgcga tcgcctctgg tggcctggtg ccttgctgac cgactctgcg gcgaaagcta
     2221 aagcgcttaa agactaccaa catgtcatgg cacagcttgc ttcctgggaa gcagaagctg
     2281 acgacgatgt ggcggcaacg attaaatccg tgcgccagca gttactcaat ctcaatatca
     2341 ctggtcgctt accggtcaaa ctggaccctg attttgtacg agtggatgag aacagcaatc
     2401 ccccgttggt cggggactat acgctctata ccgtacagcg tccggtgact attactctgc
     2461 tgggtgcggt ttctggggct gggcaattgc cctggcaggc aggccgtagc gttaccgatt
     2521 atctgcaaga tcacccccgc cttgcaggcg cagacaagaa taacgttatg gtgattacgc
     2581 ctgagggtga gactgtcgtc gcccctgttg ccctgtggaa taaaagacat gtggaaccac
     2641 cgcctggcag ccagctttgg ttgggctttt ctgcgcatgt actgcccgaa aaatatgctg
     2701 atttaaatga ccagattgtg tctgttttaa cgcaacgtgt tcctgactga tgaagaagaa
     2761 ttcttatctt ttaagctgcc tggccattgc cgtctccagt gcctgtcatg ctgaagtatt
     2821 aacctacccg gatccgctgg gttcgtcgca atcagacttt ggcggcacag gattgttgca
     2881 gatgccaaat gcgcgcatcg caccggaagg tgaattcagc gtcaactacc gggataacga
     2941 tcaataccgg ttctactcca cctccgtggc gctgttccca tggctggaag gcaccattcg
     3001 ttatacggat gtgcgcacac gcaaatatag ccagtgggaa gatttcagcg gcgatcagtc
     3061 atacaaagac aaatcattcg attttaaact tcgcctgtgg gaagaaggtt actggctacc
     3121 gcaagtggcg tttggtaaac gtgatattgc tggtacgggt ttgtttgacg gtgaatatct
     3181 ggtggccagc aagcaagcgg ggccatttga tttcaccctc gggatggcat ggggctacgc
     3241 cggtaatgcg ggcaatatta ccaacccgtt ttgccgggtg agcgataaat attgtcatcg
     3301 cgcagagtct cacgatgcgg gcgatatcag ctttagcgat atctttcgtg gcccggcttc
     3361 catctttggc ggcattgagt atcaaacgcc gtggaatccc ctgcgtctga aactcgaata
     3421 cgacggcaac aattaccaga atgatttcgc tggcaaactg cctcaggcaa gccatttcaa
     3481 cgtcggtgca gtttatcgcg ctgccagctg ggcagacctc aacctcagtt atgaacgcgg
     3541 aaacacgttg atgtttggtt tcactttacg gaccaatttc aacgatctgc gccctgccct
     3601 acgcgatacg ccaaaaccgg cctggcagcc tgcgccagag tctgaaggtt tgcagtacac
     3661 cacggtagca aaccaactta ccgccctgaa gtataacgcg ggctttgacg cgccagaaat
     3721 tcagctacgc gataagacgc tgtatatgtc tggtcagcaa tacaaatacc gtgactctcg
     3781 cgaagcggtc gatcgtgcca accggattct ggtgaataac ctgccgcaag gcgttgagaa
     3841 gattagcgtg acgcaaaagc gcgagcatat ggcgatggtg actaccgaaa ccgacgtagc
     3901 cagcctgcgc aaacagctgg ccggcacagc gcctggtaaa tcagagcaac tgcaacaaca
     3961 acgtgttgaa gcagaagatc tttctgcctt tggtcggtgc taccgtattc gtgaagatcg
     4021 ctttagctac tctttcaacc caacactttc acagtcgctg ggcggcccgg aagatttcta
     4081 tatgttccag ctggggctga tgtccagcgc ccgctactgg tttaccgacc acctgctgct
     4141 tgatgacggt attttcacca atatttacaa caactacgac aagtttaagt cttcactgtt
     4201 acccgcggac tctaccctgc cccgcgtgcg cacgcatatc cgtgattacg ttcgcaatga
     4261 cgtttatctc aacaacttgc aagcgaatta ctttgccgac ttaggcaatg gtttctatgg
     4321 ccaggtgtat ggcggttatc tggaaacgat gtatgccggt gtcggttccg agcttcttta
     4381 tcgcccgcta gatgccagct gggcgctggg tgtggacgtt aactacgtga agcagcgtga
     4441 ctgggacaac atgatgcgct tcaccgatta ttccacgccc acaggtttcg tgacggctta
     4501 ctggaacccg ccgacgctca atggcgtact gatgaaactt agcgttgggc aatatctggc
     4561 aaaagataaa ggcgcaacga tcgacgtcgc caaactcttt gacagcggcg tggcggtagg
     4621 ggtatgggcg gcaatcagta acgtatctaa agatgactac ggcgaaggcg gctttagtaa
     4681 aggtttttat atctcgattc cgttcgactt gatgaccatt ggacctaacc gcaaccgcgc
     4741 ggtggtttcg tggacaccat tgacgcgtga tggtggacaa atgctgtcac gcaaatacca
     4801 gctcgatcca atgacggcag agcgagaagt accggttgga caataacctg acgtaattca
     4861 taacattaca ttgactgata aatataaacc aatgaaaaag aacattttta agttttcggt
     4921 actgacattg gctgttctgt ctttgacagc atgtaccctt gttcctggtc agaatttatc
     4981 gaccagtaac aaggatgtca ttgaactacc tgacaatcag tatgacctgg acaaaatggt
     5041 caatatctat cccgtcacgc caggattgat tgatcagctg cgcgccaaac cgattatgtc
     5101 gcaggctaac ccggagctgg agcagcaaat cgcaaactat gaatatcgta tcgggattgg
     5161 tgatgtgttg atggttacgg tctgggatca cccggaacta accacccccg cagggcaata
     5221 ccgtagcgcc agtgataccg gcaactgggt taacgccgat ggtgcgattt tctaccccta
     5281 tattggtcgc ctgaaagtgg ccgggaaaac acttacccag gtacgcaacg agattacagc
     5341 gcgtctcgat tcagtcattg aaagcccgca agtcgatgtc agcgtcgcgg cgttccgatc
     5401 gcaaaaagcg tacgtcaccg gcgaagtcag caaatcgggt cagcagccga ttaccaatat
     5461 tcctctgacc atcatggatg cgattaacgc cgcgggcggt ctgaccgcag atgcagactg
     5521 gcgcaacgtg gtgctcaccc agaatggcgt aaagaccaaa gtgaacctct atgcgctgat
     5581 gcagcgtggc gacttgcgcc agaacaaact gctgcacccg ggcgatattt tatttattcc
     5641 gcgcaacgat gatctgaaag tcttcgtgat gggtgaagtg ggtaaacaga gcacgctgaa
     5701 aatggaccgc agcggtatga ccctggcaga agcgctgggt aacgctgagg gtatgaatca
     5761 ggatgtcgct gatgcgacgg ggatttttgt tattcgtgca acgcaaaaca agcagaatgg
     5821 caaaatcgcc aatatttatc agctgaatgc gaaggacgca tcggcaatga tactcggaac
     5881 ggaattccag cttgaacctt acgacattgt ttatgtcact accgcgccgc tggctcgttg
     5941 gaaccgtgtg atttctctgc tggtaccgac gatttccggg gtgcatgacc tgacagagac
     6001 ttcacggtgg attcaaacat ggcccaacta aaatttaact caatcctagt ggtttgtacc
     6061 ggcaacattt gccgttcgcc cattggcgag cgcttgctgc gtaaacgtct gccgggcgta
     6121 aaagtcaaat cggcaggtgt tcatggtctg gtaaaacacc ctgcagatgc gacagcggca
     6181 gatgttgccg ctaatcatgg cgtatctctt gaaggacatg ccggacgtaa gctcactgct
     6241 gagatggcac gaaattacga tctgattctg gcaatggagt cggaacatat tgctcaggtt
     6301 accgcaattg ctcccgaggt ccgcgggaag acaatgctgt ttgggcaatg gctggaacag
     6361 aaagagatcc cggatcccta tcgtaaaagt caggacgcat ttgaacatgt ctacagtatg
     6421 ttggagcgcg ccagtcagga atgggcgaag cgcctcagcc ggtaatttga gtttataaaa
     6481 tacgatgaca actaaaaata tgaatacgcc accaggcagc actcaggaaa atgagatcga
     6541 tctgcttcgt ctggtcggcg agttatggga tcaccgtaag tttattatca gcgtgaccgc
     6601 gttattcacg ctgatcgctg ttaagcacac caatttatca ggcagatact ctggtccagg
     6661 ttgagcaaaa acagggcaac gccattctca gcggcctgag cgatatgatc cctaactcat
     6721 cgcccgagtc tgcaccggag atccaactgc tgcaatcgcg catgattctc ggtaaaacca
     6781 ttgctgaact gaatctgcgc gacatagttg agcagaagta ttttccgatt gtgggtcgcg
     6841 gctgggcgag attaaccaaa gaaaaactag gtgagctggc gatcagctgg atgcatattc
     6901 cacaactgaa tggtcaggat cagcaactga cactcacggt tggggaaaac ggccactata
     6961 cactggaagg tgaagagttc accgtcaatg gtatggtcgg ccagcgtctg gaaaaagatg
     7021 gcgttgcgct gactatcgcg gacattaagg ccaaaccagg aacacagttt gtcctgagcc
     7081 agcgtaccga actggaagcg attaacgcat tgcaggaaac ctttaccgtt agcgaacgca
     7141 gtaaagaaag cgggatgctg gaacttacca tgactggtga tgatccccag ttgattactc
     7201 gtattctgaa cagcatcgct aacaactatt tgcaacagaa tatcgctcgc caggcggcgc
     7261 aggattcaca aagccttgaa ttcttacagc gccagttacc tgaagtgcgc agcgagctgg
     7321 accaggcgga agaaaaactc aacgtttatc gccagcagcg cgattcggtt gaccttaacc
     7381 tggaagccaa agccgttctt gagcagattg tgaacgttga taatcaactc aatgagctga
     7441 ccttccgcga ggcagagatc tcccagctgt ataagaaaga tcacccaact tatcgtgcgc
     7501 tgctggaaaa acgccagacg ctggagcaag aacgcaaacg cctgaataag cgggtatcgg
     7561 caatgccttc cacccaacag gaagtgttgc gtttaagtcg tgacgtagaa gcgggccgtg
     7621 cggtatatct gcaattactt aaccgccagc aggagttgag tatttcgaaa tccagtgcca
     7681 ttggtaacgt gcggattatc gacccggcag tcactcagcc gcagccagtg aaaccgaaaa
     7741 aagcgttgaa tgtggtgctt ggttttattc ttggcctgtt tatttcggtg agtgccgtgc
     7801 tggcgcgtgc gatgttgcgt cgtggtgtag aagccccgga acaactggaa gagcacggca
     7861 tcagcgttta ttccactatc ccaatgtccg agtggctgga taaacgcacc cgtctgcgta
     7921 agaaaaattt attttctaat cagcagcgcc atcgtactaa aaatatcccc ttcctggcgg
     7981 tggataaccc ggcggattct gctgtggaag ccgtacgtgc gctacgaacc agtctgcatt
     8041 tcgctatgat ggagactgag aataacattc tgatgatcac cggtgcgacg ccagacagtg
     8101 gtaaaacgtt tgtcagttca actctggcag cggtgatcgc ccagtccgat caaaaagtgt
     8161 tatttattga tgccgactta cgccgtggtt attcgcataa cctgtttacc gtgagtaatg
     8221 aacatggctt gtcggaatat ctggcaggta aagatgagct caacaaagtg atccagcatt
     8281 ttggcaaagg agactttgat gtgattactc gcggtcaggt gccacctaac ccgtctgaac
     8341 tgctgatgcg cgatcggatg cgtcaattac tggaatgggc gaacgaccat tacgatctgg
     8401 tgattgtcga tacgccgccg atgctggcgg tgagcaatgc tgcggtcgtg ggacgctctg
     8461 tcggcaccag cctgctggtt gcgcgttttg gcttgaacac cgccaaagag gtgagcttgt
     8521 caatgcagcg tctggaacag gctggcgtta atattaaagg cgcaatcctc aatggtgtga
     8581 ttaaacgcgc cagcaccgct tacagttacg gctataacta ttacggttat agttactccg
     8641 agaaagagta aaactgaaag tgaccggatg cggcgtgaac gccttttccg gtctacgcat
     8701 tagacagttc ttcgtttgtc atcagcctca gagcattcag gtaactgaat gctctttttt
     8761 atgcattaca aactgcacgc cggtatgcgt gcttcattca cgatttgcgt aaaacctgcc
     8821 aacgaacaca tgccctgcgc atttcgctct tcacatcctg ccagggtcag tttcacctct
     8881 ccgggcggcg tatttaatga cagcggcgtt ttatcacgca tctgctgtaa agtctggaag
     8941 accagcgaaa cctgaatcca ctggctgtta tcgcttagcc gacgccagcg ttcaaacacc
     9001 agttcaccac ctggcggcgt gttatccggc tgacctggaa gcgtccagtt gagctccagt
     9061 gcgccgccga gatttgccag attagtatcg tgtccggcaa taaacagcac tgaagtgggt
     9121 aatgtcacac catacgcctg tttttgcggt ggatggggcg tcaacgctgc catgatcaaa
     9181 tccaataacg gggtggcgcg actgcgggca acctccggcg tgcgttgtag taaataaaat
     9241 tgcgcgttat gcaaacttag caaggtgttc cactggtgtg aatcggtgat ccttccccac
     9301 cccggctccg gcattccttg tgcctgttgc aggagaaata tctccgtcag cattgatgcg
     9361 aggcttaccg caccggttaa tgagacattg tcggcgctca ccttgagttc cgatggtaat
     9421 gcctgcgtta atgaacagct ttcgtcctgt ttctcacggt taaggcacaa gtttgattgc
     9481 gggaaattaa gcacccgttc cagttcgcga aacaccgttt gccgatgccc ggtaaagtca
     9541 gcaattgacc ctcctgccct gcagaggatc gcgtcagtca cgttcgcgtt atccagttgg
     9601 caaacaccag tttttagagg attaaataac ggatcgggac tggacgtatc tgcctgggta
     9661 tgtacggtta ttgcacagtc aggtgccagc ccggcggcga aggcttcgcc tgttttacgg
     9721 gtacgctcgt cgacatcagc aataatcgcg acctgagcag actgcgggca gccctttttc
     9781 gccagcaatc cgtcggctac cagacgctgg cgttggtaat gtccgagata ggcgattagc
     9841 tcaccaccgc gcggtgtcag ccaacccagt tttaccggcc aggttggcca tgcgtctggg
     9901 gtgacatcct gcatcagttg cgtggccttg gttggagcac gcacaccatg acgactgaca
     9961 atcaccatac tttccagctt cagctccggc tcactctgag cgaatgcaga ttgcggggtt
    10021 aacggaatca gaagagataa aaatgggatt aagatcgctt tcatcgatat gtttccgctt
    10081 ttcatatcta acattattga ttgcctgatg cgatgctaat gcgccgcatc aggcctacgt
    10141 tcgttaactt ttcagacgcg ggtccagcca taccaacaca agggtggaga gcgaacacat
    10201 caacaaaatg ccgacgaacc aaagtaaata ccacatgttc ctgctcctta gtacaactcg
    10261 tttttgttac ggcggagagt ttctgttgtc atgcgtcccc acattttgta gtagctccag
    10321 agagtgtaga gcaacacaat gggcaaaaat atcagcacga ttaccaacat aatgctcagc
    10381 gtcagctgac tggaagtgct gtcccacaac gtcaggctgg agattggact cacacttgac
    10441 ggcatgacaa aggggaacag cgtgatgcct gccgtgaaaa tcacgccaaa ttgcatcaat
    10501 gatgccatca aaaatcccca acccgggcga ccacgataaa tcgccatcac cgtcagcaat
    10561 gggcagaaga accccagcag cgggaagatc cacaaaacgg gcgattcgac gaaattattc
    10621 atccaggcac caggtagcac tgccaccagt ttcattaacg gatgggaagg accgttagca
    10681 tcctgggcga gcagtacaaa gccatcaata ccgacccaca gccagtaacc tgccagcaaa
    10741 aagcacagca tcaccaacag tgcagcgcgt ttagtcgcca gctgtgaacg cagatgaatc
    10801 acaccaacag ttttcagttg taaccagacg ccaccttgca aaatcaccat cccaaggctg
    10861 agcaaaccac acaataaagg gaatggcgtc agcagttgcc agaagctgcc gagatactcc
    10921 acgcgtaatt gcggtgtgaa ggcaaacggc acgccgagca acaagttgcc gaaggcgata
    10981 ccgaagacta ccggcggcac cagactgccg atgaccagac cggcatccca cattttacgc
    11041 cagcgtgcat cggcaatttt tccgcgataa tcaaaggcca gcggacggaa gaacagcgag
    11101 cacagcacca ggatcatcgc cacataaaag ccggaaaacg ccgctgcata cactctgggc
    11161 caggctgcaa ataatgcccc acctgcgagg atcaaccaga cctgattgcc ttcccagtgc
    11221 gcaccaacgc tattaatcac tatccggcgt tcatcatcat tacgcgccac cagcggcagc
    11281 aaacagccga tccccatgtc aaatccgtcg gagatcataa agaccaccag gatcacgcca
    11341 atcagcagcc accagatgaa gcgcaatgtt tcataatcaa acatgatttt ctcctttacc
    11401 cctgttgctg cgtcggttgt tcactctgca tcgcgctcgg ccccagacgg gcatatttct
    11461 gcatcaggta gacttcggcg attaagaaca gggtgtaaag ccctacgatc atgatcagtg
    11521 agaaagccag ttgtcctgtg gttaaagcgg agtgcgcgga gtatgtcggt aagatgtcct
    11581 gtatcgccca cggctgacga ccaaactcgg tcataaacca cccggcttca atcgcaatcc
    11641 acggcaacgg caaactccag agcgccattt tcagcaccca gcgatgctgg tcgattttgc
    11701 cacgcagcgt ctggacaagc gcaatcagca tcaccagtag cagcagggaa ccacagccca
    11761 ccatgatgcg gaaactccag aataccggcg caacctgagg tatcgcgcca cgcatcgccg
    11821 cctggtactg tgcggctgtg acatgattca tatccggcgc atagcgggag agcaacatgc
    11881 cgtaaccaag gtcgccttcc agctcccgga atgcctgcaa cacatgcggc tcacgattgc
    11941 cttgagatat ttcctgcatt aacagccagg ccatacgtcc gcgttgcaag cgtgggtagg
    12001 tttcagccat caaattcttc agacccggca cgggtttatc taatgagtga gtggcgagga
    12061 tccctagcag cgcgggaatt ttgatggcaa aggcgttacg ctcttgatcc tgttccggcc
    12121 aggcaaccac atggaacggt gcaggagcag gttccgtttg ccactcccct tccatcgccg
    12181 ccagttttac cggttgtact tgcgcgactt catacgcaga actgtctccg agttgcaggg
    12241 taccgataat cgccagagta ccgaagacgg aaccgatggc aaacgagcgt aatgcgacat
    12301 cgcgctcccg tccgcgcagt aaataccagg cgctgatcgc cataataaac atggccccgg
    12361 tcacgtagcc cgccatgacg gtgtgcacaa atttcacctg gctgaccggg ttaaagacca
    12421 gctcgctgaa gctggtcatc tccatacgca gggtgtcgat atcaaaatgc gcaccggtcg
    12481 ggtattgcat ccaaccgttg gcattcaata tccacaatgc agagagattt gaaccgaacg
    12541 ccaccagcca cgtcaccagc aggtgctggt atttattcag ccgttgccag ccgaagaaga
    12601 acagcccgac aaaggtggat tcgaggaaga aggccattaa tgcttccatc gccagtggtg
    12661 cgccaaaaat atcgcccaca tagttggaat agaatgacca gttagtacca aactgaaact
    12721 ccatggtcag gccggtagcc acgccaagag caaaattgat accgaagagc ttaccccaga
    12781 agcgcgtcat atcgcggtag attgttttgc cggtaaccac gtaaatggtt tccatgatag
    12841 ccagcaaaaa aatcagcccc agggtaaggg gtacaaataa aaagtgatac agcgcggtca
    12901 gagcaaactg ccagcgcgat aaatcaatga catcccacat gcgcactcct gtaggcgaag
    12961 agagaaaaat aacccgataa tgttagatct ccctctttat cctgaaactc tacgaccaga
    13021 ggtattcgcc gattgcgata ttcataagac tgaaagcgat acttaccgtc tttttacgtc
    13081 ggtgcagctt ccgccagcca ctgacatacc tcgctaagcc gctgcgcaga gtcgaccaaa
    13141 tcttctggcg ctgccagcac cacttccggt attgggcaga tttcataact ttccagtaac
    13201 gggcctttta aggtgtccgt acagcgtaaa tgccagacat ggcgtaaccc cgtggcgttg
    13261 atatagctct cgccatagcc aatggtacga atctgaatat ttcccggccc acagagacgc
    13321 gagagaaaaa gccgatcagc ctcgctgatg ggtaattgcg tcagattaat gctgtgcggc
    13381 tgcgcgtcgg ggttacgtac atgtgccagt aactcatgcg ccaacggtag gccattcatc
    13441 aggccatcaa tgggcggcgg taacagcgaa tctgtcggca agagattttg cgttgccgcc
    13501 tgccacaacg ccagcggcgc gcagccagcc tccagtttgt cctccagcaa cttttcgccg
    13561 cgacgtctgc gcacccgcca taatccgcag aagatcgcct cctgtatttc acttttactg
    13621 tcgtcagcct gctgaatgcg cactgacact tccccttccc cgagaagcgt attgataaag
    13681 tgacgatcgt cggcgttgag atttaacaga tcgactgcca gcacctcgcc caacggcggc
    13741 tggcgttcgc ttagttgatg ttgtaactcg tttaacagcg caatcacctg cgggctgtga
    13801 gcacattgct ccaaggccgc catactcggt tcatcattca cctgacaggt gatcggcagt
    13861 ggattcatgc tgaaactgtc atcgttcggt tgcgttcctg gccccagcag atggaaaaaa
    13921 gtttcgctca tgaggcacgc tcctgctgcg gttcgacaag cccgcgcatc aggtttatca
    13981 gttccgccca cgggtgaata ccattcagca cgccgcgata gtttccgccg gtaaacacta
    14041 aagtggcagg aaagcgaaag gcgccaaagc gatcgccgat ggcttcgctc tgctcaaggt
    14101 cagcaatcgc cacctgccat gtatagtcgg gaaactcgtg cagtaattcg ccaatcatta
    14161 ccggattatc gctgacctct ggcgtgcgtt tcgggtcact gcttaataac accacgccgt
    14221 ctggcgcttg cgtaagccag tcgtcaagac gggattcact gaccggcgtc cagccgcgcg
    14281 ccagcattcg ttgccacaac gcatcaaatg gcgtgtcgtt gctcatccct gctcctcctg
    14341 tgtcatccgg tactggcgca agcgaacgcc ttcgtaattt tccatcgaca gacagtcata
    14401 attgagacaa cgcgattcat tagccggttg cggcacactt ccccacgcag ccaactgcgc
    14461 cagcgccgat tgttccgcag cgggcagttg ctcccgtgcc agttcgctca ggctgccgcc
    14521 gtagtcgtcg agcatcgcgg gttgcagccc gacgagggca atatgtgctg gcagatgtcc
    14581 gcggatatct gccagcgcca gcacttcgga gaaactgttc tgatgcaggc tcattttctt
    14641 cgcgctgaga taggccggaa tgcgttctcc ggcataggtt cgcagcgttc caggttccag
    14701 cccgtagtca atggcatcga gaatcaacag atggctggcg ctttcgacat accccagcaa
    14761 gttcagtccc tgagtaccgc catcgacaat ctccacatac tcgggccagt ggtaatgggc
    14821 atacagccgt tccgccaccc gcacgccgaa gccttcatcg gcccacagca ggttgcccag
    14881 ccccatgacc accacgtgtt gctcgctcat gaacgctcct tgttacttat tttgccaaat
    14941 ttgtggctac ggtagccgtt gaccatggtg gagatcaccg tgtcgtcgga catgatgtct
    15001 tcacgcagcg ccatgtagac atgaccgatc acaaacgccc caatcagcca catccccagc
    15061 cgatgccagc tgtgaatgtc cattgagttg ccacccgtcc agtagaaaaa ttccaccaca
    15121 taacggaacg gcgcaaaaat agcgtactgg ctgtgttcgc tgtacagcgc aaaaccggtg
    15181 atgatcataa agaccgacat caggaaatag ccgaacatcg ccgcctgggc gatggggtta
    15241 tggcctatat cggcactcgg acgttttgcc agaaacagat accagcggat ttcataccac
    15301 acgccctgcc accagctttt acgccatacc ggcacgataa acagctcgcg ggagtaacga
    15361 ttgccaacaa aagcccagta gatccgcatc agcaagacca cggtaaaaat catcccggcg
    15421 ctgaagtgaa ttaacctgat gtaacccata tagaacagat acgtcgcctc gccactgacg
    15481 gaaggtagcg gcttgccgat aaagtaaccg gtgaccatca acaccgccat gcataacacc
    15541 gtcagccagt gccagatacg aactggtgct tcaaagacat agtggctgac aacgttgtcg
    15601 cttttctgtt gcatgatgat tctccttcgc tgttaacgca cctgcacgga gatcagctcg
    15661 ctaccgtcgt cgcccagcac atgagttgaa caggcgaggc acggatcaaa gctgtgcaga
    15721 gtacgcagta tctccagcgg ttgctcgggg atcgccattt tggtgttcat cagcgccgct
    15781 tcataagcgc caatctgccc tttgggatcg cgcggactgg cgttccaggt ggtcggcacc
    15841 acgcactggt agagatcaat cttgccatcg cgaatggcgg cccagtggcc taacgctccg
    15901 cgcggcgctt cggtaaagcc gacgccacgg cactctgtcg gccaggttgc aggttcccat
    15961 ttttcagtgg aagcagtggc gagattgccg tttttcaggt tggtcatcag cttgtcgaag
    16021 aaatactgca acttacctgc ggcccactgc gcttcgtgcg cgcggcacaa aatgcggccc
    16081 aacgttgact ggataccgga aagcggcagg ttcaacgccg acatcatgcg atcgaccgac
    16141 tcaacggtcg cagcatcgcc tttgtgataa gcgattaacg tgcgtgccag cggccccact
    16201 tccatcgcgt taccgcgcca gcgtggcgct ttgatccacg agtagcgttc ctgttcattc
    16261 agctgctgaa tgttggtatc gctgcctttg acatcgccgg ggttgtacca cgggtcggta
    16321 atgccatcga atggatgacg cccgacctga tcgttgggat aacgatacca ggcgtgatcg
    16381 acaaactcct gcacctgctg cggatcaacc aaatccactg gcagcacatt gttgaagtcg
    16441 ccgttaatca ccgcgccgcc aggcatcagc agacttttct caccaaagtc gttggcaata
    16501 tccgggaatg cgccgtagct gaggacacat ttatcagaaa gacccgtgcc gatttcgctc
    16561 cacggtttgt tgaactgacc gatggctaag gcgtcgggga tcatcacgtt gttaatgaag
    16621 tctgccgtgc gggtgataat cgactgcacc aggttcaggc gttccatatt gaccgccccg
    16681 accgcgccgc tttcgtcaat gttaatggcg caaggcatcc caccgacaat ccagtttg
//
LOCUS       DAJTSI010000088        16628 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000088 DAJTSI010000000
VERSION     DAJTSI010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16628
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.088"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(157..1392)
                     /locus_tag="OO327_002583"
     CDS             complement(157..1392)
                     /locus_tag="OO327_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR6019081.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1539..1615)
                     /locus_tag="OO327_002584"
     tRNA            complement(1539..1615)
                     /locus_tag="OO327_002584"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1579..1581),aa:Pro,seq:tgg)
     gene            complement(1658..1744)
                     /locus_tag="OO327_002585"
     tRNA            complement(1658..1744)
                     /locus_tag="OO327_002585"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1708..1710),aa:Leu,seq:cag)
     gene            complement(1765..1840)
                     /locus_tag="OO327_002586"
     tRNA            complement(1765..1840)
                     /locus_tag="OO327_002586"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1805..1807),aa:His,seq:gtg)
     gene            complement(1899..1975)
                     /locus_tag="OO327_002587"
     tRNA            complement(1899..1975)
                     /locus_tag="OO327_002587"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1939..1941),aa:Arg,seq:ccg)
     gene            complement(2078..3463)
                     /locus_tag="OO327_002588"
     CDS             complement(2078..3463)
                     /locus_tag="OO327_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6019082.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3654..4394)
                     /gene="rffM"
                     /locus_tag="OO327_002589"
     CDS             complement(3654..4394)
                     /gene="rffM"
                     /locus_tag="OO327_002589"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR6019083.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4397..5749)
                     /gene="wzyE"
                     /locus_tag="OO327_002590"
     CDS             complement(4397..5749)
                     /gene="wzyE"
                     /locus_tag="OO327_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR6019084.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5746..6825)
                     /gene="rffT"
                     /locus_tag="OO327_002591"
                     /gene_synonym="wecF"
     CDS             complement(5746..6825)
                     /gene="rffT"
                     /locus_tag="OO327_002591"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR6019085.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6822..8072)
                     /gene="wzxE"
                     /locus_tag="OO327_002592"
     CDS             complement(6822..8072)
                     /gene="wzxE"
                     /locus_tag="OO327_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR6019086.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8074..9204)
                     /gene="rffA"
                     /locus_tag="OO327_002593"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8074..9204)
                     /gene="rffA"
                     /locus_tag="OO327_002593"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR6019087.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9209..9883)
                     /gene="rffC"
                     /locus_tag="OO327_002594"
                     /gene_synonym="wecD"
     CDS             complement(9209..9883)
                     /gene="rffC"
                     /locus_tag="OO327_002594"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR6019088.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9861..10742)
                     /gene="rfbA"
                     /locus_tag="OO327_002595"
     CDS             complement(9861..10742)
                     /gene="rfbA"
                     /locus_tag="OO327_002595"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR6019089.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10761..11828)
                     /gene="rffG"
                     /locus_tag="OO327_002596"
     CDS             complement(10761..11828)
                     /gene="rffG"
                     /locus_tag="OO327_002596"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR6019090.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11828..13090)
                     /gene="wecC"
                     /locus_tag="OO327_002597"
     CDS             complement(11828..13090)
                     /gene="wecC"
                     /locus_tag="OO327_002597"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR6019091.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13087..14217)
                     /gene="wecB"
                     /locus_tag="OO327_002598"
     CDS             complement(13087..14217)
                     /gene="wecB"
                     /locus_tag="OO327_002598"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR6019092.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14273..15319)
                     /gene="wzzE"
                     /locus_tag="OO327_002599"
     CDS             complement(14273..15319)
                     /gene="wzzE"
                     /locus_tag="OO327_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR6019093.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15331..16434)
                     /gene="wecA"
                     /locus_tag="OO327_002600"
     CDS             complement(15331..16434)
                     /gene="wecA"
                     /locus_tag="OO327_002600"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR6019094.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
BASE COUNT         4280 a         4685 c         4053 g         3610 t
ORIGIN      
        1 tgccaccgca cgtaaaggct ttgccggata cgatcaccca aaccagtatc cacagaacgc
       61 tgcaatttat tgaatttgca cgatcatgta ggccggataa ggcgtttacg ccgcatccgg
      121 caatcaaccg caggcggctt ccaaatggcc gcttttttac ttattcacca ccatcaaatg
      181 tgcctgcata atgtcactgg ccgggcgacc gtgcgccagc aaatcagcca ttgctttaag
      241 atatggcggt agatggcgga aataacgctg atacccggca cacaaataat tcagccccgg
      301 tttgccgctg gcatcgagca tgaagcggtg tttcgggcag cctccccaac atgcttttaa
      361 cacgttacaa ctgcgacact gcgccggtaa ttgcttaaat ttatcttcac caaacacctg
      421 ctgttgcggg gaatcgatca tttctgcaat tgtttgctga tgcatattcc ccagccgata
      481 ttgcggataa acatagtgat cgcaggcgta aacgtcgccg ttgtgctcaa caatcaccga
      541 gcgcccacag gttggctgat gatggcaaac cgcacccggc gcaccgacaa aattggcaaa
      601 cgcccattcg atattcatca cgaaaatctt gccgacgtcg cgtttgatcc agtggtcaaa
      661 aatcgccacc agaaattcac caaattcatc ggggcgcaca gaccattccg tcagttcccc
      721 ctgaatatca ccaggcgcat gtagtttcag tccatcgcgg acagctgttt catcagccag
      781 gcgctcgacc accggaataa actggatgaa ttcgactccc gcatcgcaca aaaaatcata
      841 aacctgtaac ggttgctgcg cgctggtgcg gttgacgcag accagcacgt tatagtcgac
      901 atgatgtttt tgcaggagcg tcagggcacg catcaccagt ttatgcgtgg gtctgccgcc
      961 tttggtcacg cgatattgat tgtggatctc agccggacca tccagcgata acccaacaag
     1021 aaaattattt tctgccagaa atgcacacca ttcgtcatcg agcagcacgc cgttagtctg
     1081 gaaactgtta cttatcttcc tgccagcacc atatttcgcc tgtagcgcca cggcacggcg
     1141 gtaaaactcc agcccgagta gcgttggttc gccgccctgc caggtaaaag ccacttcgtt
     1201 ttgcagttcg ctggcggcga tatagtgacg gatatacgct tccagcgtgt catcgtccat
     1261 atgcgtgact ggcttttcgc ggtaaaggga ttgtttttcg agataaaaac agtagtcaca
     1321 gttcagatta caatcggaac cgctcggttt cgctatcaca tgaaaagcac gcgttggaac
     1381 ctgttgcagc atggtcgctc cttgcctgaa gtcatccttc aatacgttaa gggcgaaatc
     1441 ataccgtggg gcaatgagtt attacgggcg tttattgcga agggagggtg acgaaatgca
     1501 cagaaaacaa aaaaccccgc cgaagcgggg ttcaaaattg gtcggcgaga gaggattcga
     1561 acctccgacc cactggtccc aaaccagttg cgctaccaag ctgcgctact cgccgaaata
     1621 ctgctttttg aatttttagt tcaattcttt aaagtcgtgg tgcgaggggg gggacttgaa
     1681 cccccacgtc cgtaaggaca ctaacacctg aagctagcgc gtctaccaat tccgccacct
     1741 tcgcattgtc acaacttcta ataatggggt ggctaatggg attcgaaccc acgacaactg
     1801 gaatcacaat ccagggctct accaactgag ctatagccac cactacaaat cttgttacgc
     1861 ggtattacta ccaccgcagc tcaagcgctg ggactaaatg gcgcgcccga caggattcga
     1921 acctgagacc tctgcctccg gagggcagcg ctctatccag ctgagctacg ggcgcttagc
     1981 gccgttgcgg gggtggataa tacggacttc ccacccctct gtctagtccc tttttaaata
     2041 aattgctcgt ttggttacgg tttgtgcgtt ttgctgctta ttcctccagt ttatgcgctt
     2101 taccgtggcg attaagccca aaaactttat aaatcgccgt caccgccagc ataaagatga
     2161 tgccgacaaa cagcgacata cgcgtatctt cattaaagta catgccgatc aaaacgcaaa
     2221 tcaggaatgc cattgttacg tagttggccc acgggaacag gattgagcgg aacggatggc
     2281 tggcaatcgc cgctttatgt gcacgccgaa aacgcagctg gcttatcaat atcacaaacc
     2341 atggcaccat ccccggaagc acgctggcac tgtagacgta gacaaacaca cgctgcggat
     2401 tgggaatgat gtagttcagg catgagccaa ttagcagaat agcaatagat actgccacac
     2461 ccgcaaccgg tacgccgtga cgggaaactt ttgccattgc cgccggtaac tgacggtttt
     2521 tcgccagtgc gtagagcata cgtccgcaac tgtacatgcc gctgttacag ccagagagcg
     2581 cagccgtcag caccacaaag ttgataatgc ccgccgctgc ggtaataccg attttggcaa
     2641 aagtcagtac gaacgggctg ccgttgctgc caatttcatt ccacgggaag atggtgacga
     2701 taacgaaaat cgcgcctacg tagaaaatca ggatccgcca cagcactttg cctacggcac
     2761 tgcgcagcgt cacctgcgga ttcttcgctt caccggcagt aatgccaatc agctccacgc
     2821 cctggtagga cgccaccaca atacacagag cggtcaggaa ccctttccag ccacccgcaa
     2881 agaaaccgcc atgctctgtg agattgctaa aaccaatcga ctgcccgcca ttgccaaagc
     2941 caaagaaaat cacgcccagg ccaatgacaa tcatcacgat aatcgtggtg actttgatca
     3001 tcgcgaacca gaactcgatt tcgccgtaca accgtaccgc cgccagattc gccaacgcca
     3061 ccagcgccac cgcgatcaat gcgggtatcc actgcgccat ctccggggac caaaactgga
     3121 cataaacgcc aatggcggtg atttctgaga tccccaccgc catccacata aaccagtaag
     3181 accaggcggt gagatagcca aaaaacgggc tcatataacg atgcgcataa acggcgaacg
     3241 aaccggtaac cggttcgagg aacaacattt cgcccattga acgcatgatg aaaaaaacga
     3301 acagcccggc gatgatatag gccaacaata cggatggccc ggcccatttc agggtactgg
     3361 cggcccccat aaacaggccg acgccaatgg tgccccccag ggcgatgagt tcgatatgtc
     3421 gagcttccag cccacgctgt agctctggtt tgttatctgc cataaatcct cgtgttgtgt
     3481 ttgcatgctt tccggtgtta ccggttatcg ttatgggtac atcgagtgtt gcgaatgttt
     3541 tcgtaattca ggagaaatgg caaataaagc attaaaaatt tgaatgcttt gtgtaataaa
     3601 aaagcagaca ggcgacgaag tgaccactcc gtcgctttac aaagagagga aaatcagagg
     3661 ttgccggtgt agtgccagcg taaataacgc agcaaacgaa gctgacgctt aatgcggctc
     3721 ggctgcgaaa gcaggcggta gagccactcc agtcccagcg tttgccagat tttcggtgcg
     3781 cgttttacgt gaccggtgaa aacatcgtaa gtcccgccaa cgcccatata cagcgcatct
     3841 ggatgcacca gacggcagtc gcgcatgaag atctcctgct ttggcgatcc catcgcaacg
     3901 gtgacgattt gcgctccgct ggcatgaatg cgttcaaaca gcgcctgacg ctgctcgggt
     3961 ttaaaataac catcctgact gccaacgata ttcacattcc attggttgcg cagtttagct
     4021 tcagtttgcg ccagcacttc aggtttaccg cccacaagaa ataccggcgt cccttctttg
     4081 cctgcgcgcg ccatcagctc ttcccagaga tcggcaccgg caacgcggga aacctgcgcc
     4141 tgcgggtact ttttacgtac tgaacgtaca atgctgatgc catccgcata tttaaattcg
     4201 gcagcgttaa ttaactccct gacctcggcg ttatcttcaa tggtcagcat tttttcggca
     4261 ttaatggcaa ccagcgttcc ctgcttaagc tgcccgtcag caaacagata atcgagggca
     4321 tgctgcatat cacgccaacc aatcaactgt aagccacgca gcgtataggt cggtgccgtg
     4381 gtgttgttat tcattgttat ccttcaacct gcgtccggag cgatgatttt gtacgtttat
     4441 gaatgagtcc ggcgctttca aaaagccagt acaacagttt tgcgatcatc agacatgcgc
     4501 cgaagaccac gataaaaaag actacgcgtg agacaaacga atccagccct tcacgtgcca
     4561 gcacgatcat attgaagatc gccccaaagc agaaactgtg caatatcgca gccttatagc
     4621 gattagtctc gcggttgccc agctcataca gccagtcgaa ccatttgatg atcagtccaa
     4681 ccacaatcgc cccgagtggg atgaacaacg cgccgcccat caccaccagt gagcctataa
     4741 gcgtaggcga gatcgccagt ccggagtggt tattcagtac ttcccaggta aagtaattgg
     4801 ctgagttcag caccatactc gggcgccccg gccacagcca ggaagggata aagacataga
     4861 aatcgcggac aattggagcc aggccctgga agtcgatgtt gtcgtagttc tgcaacagca
     4921 acgccagatt ctcccacggt gaaaaggtgt cgcgagtgag atagagaaac gtatagaacg
     4981 cttcatcgcc gctcacattc attccatagc gttttaacgc cagccagaac atgccaacaa
     5041 tccccagcac gcccgccgcc gccagcatcc acaacgaaat ccagccgcga ataatgccaa
     5101 taaacaggaa gatagcgaat gcgatgatga tattggcgcg agtgccgccg acaatcatat
     5161 aagtcagcaa gccaaaggcg accgtgctga cgaggaaaaa cagccacgct ttgctgtcct
     5221 ggcgcagaaa gtagaccacc agcatcgccg ggatgaaaaa gtaaaagaag cgttttaacg
     5281 ccacgccgga gacttcactg gaaaagatct gactgtagga gttaagccgg aacagcaaaa
     5341 agccgttgtg catgaagaag atgccgacgc ttaccagcgc gatacccatc aggatcaccc
     5401 acgtaagatt ggtctccacg cggttcatgg taaacagcgg acggcgcggt acatcagcaa
     5461 cgcgtttgcg taggcgggtt ttgtaggtga catagtaaac cgcgtagaag cagcccgcag
     5521 aaagcaacac ctgcaacaag atttctggcg gcgcgacacc aacatcaaag cgaaatacca
     5581 gcacgctggt cagcgggaag ccgaagaaaa aggtgagcaa aaacagcaat gaaaagaaga
     5641 cattgaagtt aaagcgcaca cggcgaaact caaaccaggt cagcgtggca ataaacagcg
     5701 tgcagagcag ccagaccaca aacaggccac tgaattgcag cagactcatg cgacctcccc
     5761 ggcggcaatc gccaacgccc gctgccagcc ttgtagatag ttagggctaa agaaggcaat
     5821 ggtgttttta tccgccgacg ccaactggcg ctgcgcttca cgcacaatat cctcgttgag
     5881 atcgtcggta gtaaacagca ctggcaaatg ctgttccgtc atatcctgcc agaacggatt
     5941 ttccctgtta agcacacaag gaatgcccgc ctgaatcagt aagcaaagcg taccaatgcc
     6001 ctgctggcgg gcaaaaataa agtaaccaag atcgcactga cgaagtagcg ccagataggc
     6061 gtcaaattcc agtttttcgc tcaaaatttg cagattttct tcgctgaata actccagccc
     6121 cgcctgacga acttcctcaa tgtacgcttc gttattaggc ggatatccca tcggcaccac
     6181 cacttttacc gtatcgccaa attgctgatg aacggcacgt aaagcggcga catgctcatt
     6241 gctgcggtcg ccggagttcc ccaccagaat ggtcattttt ccttcacgtt gccgatcgtt
     6301 cgccatcgta ttgagagaag ggtccatccg cgtcgggaag tacagcagtt ctccccgcac
     6361 ctttgggtgt gttttggcaa aaaagctcaa atcgccgcgg gtggcaaata cacagccgac
     6421 tcgcttttgc gccaggcgac gtagtgggta aaaaagctta tatctcaagc cactggaaag
     6481 ctcatacagg tctgcccccc agatatgcca gaaaaactgg ctgggcttaa tgccaccact
     6541 cagcagagcc agccacagtg tgggattgaa ctgaccgtgg aaaaaaaatc gctgctgacg
     6601 gttagctttt gctttcgcga tgaccgcttc cgccagcgat tttttcccag ggaaaaattg
     6661 cacagaaagc gccggacagc tatcacttaa gccgtcgtcc ttgccaacaa ccataaactc
     6721 gcgcgcgtgc tcgctcgtcg cggccagcgc gtcattgaaa aaccgcaaaa cggttcggtt
     6781 atggtgaggg atatccgatc ccagtacgtg aatcagtaca gtcatgcccg cctacgccag
     6841 agtaaaaaca cgccacaaca aagagaaaaa tagacgatat aagttgccat atatgcctgc
     6901 gccgcgccca gtgcaccatg cgcagggatt agccagtggg caaataccat caataaagtg
     6961 aactggctga cttccgccag aatataaaac cgcagtgacg ctttggcgat caccagataa
     7021 ccaaagacat aagcgcccac ttttaacaca tcacccacta actgccaggc aaagagatcg
     7081 cgcatagcgg taaatttatt cgacaacagc aaccagatag caaaatcacg cagcaaccag
     7141 acggtaaaac tcgctgccgc cactgccggt aagacgaatt tcagcgattt aaccacttcc
     7201 cgggtgatat cgcgcttttc cgttagccgc gacaacgtgg gcagcaaata tacgctgaac
     7261 gatgccgtaa taaattgcag gtaggcatcg gaaatactgc tcaccccttg ccagatcccc
     7321 acttcatccc agctatactg cgccgccagc agtttacgca tcatgatgta agcaacaggc
     7381 aaggtcaccg acgtaatcaa cgccatgagc gtaaatttgc tcaactgccc tgccagaccg
     7441 ttatcccagc tgggtttcag atagcttaac gggatgacac cacgtttaat caacatgatg
     7501 gcggcaggaa ttaccaccag cgcgggaatc agcgccagac ccagcaacgc cccttcataa
     7561 ccgcccaaac ggtaactgac gtagtacgcg agaacgccaa tcaagctgcc gacaatcagc
     7621 gataacgcat tacctgcggc atcgcgaaag cctttcatca gcgccagtaa caggttgccc
     7681 caggcgatcc ccatttgcac cagcgccact aaacgcacca gcccctgata gtcggtatta
     7741 ccaaacaatc cctggctgat tggcgcagct gccagcacaa aaaccagcgc catcagcgta
     7801 gagaaaccaa gtaccatcgc tgatgaagtg ccgaccacgc ggcgcagctg ttgcggatta
     7861 tcatggtact gggcaacgta tttggttaca ccgttaaaga tgccagcccc ggcaagcacg
     7921 ccgagcacgg taatcaactg gcggaaattt gccgccagcc caagccccgc cggaccaaat
     7981 gacaccgcca gcaacttacc gaccagtaac ccggcaccaa tcttgaccag tgtactggcc
     8041 gccgtccaca aggacgcttt tgccaacgac atatcaggag aagtagttca acaaagtcgc
     8101 aattaccgta cgctgattga cgggcgacag gttgtagaac agcggcaggc gcagcaggcg
     8161 ctcgctctct ttggtggtgt agcgatcttc accgtggaac tcaccaaagt gttcccccgc
     8221 agggcaaccg tgcagcggaa tgtaatgaaa caccgccatg atttccgctt ctttcagaaa
     8281 gttaatcaac gcgctccggt catcaatatc ccgcagttta atgtagaaca tatgcgcgtt
     8341 ctgcacgcag ccatcgggaa tcgacggcag ctcgatacgc ccggctttcg ccagaggcgc
     8401 taacgtatcg tagtagtttt gccacagcgc cagacgttgc tggttgatac gatccgctgc
     8461 ttccagttgc gcccacaggt acgcagcttg cagatcggac atcaaatagc tggagccaat
     8521 atcgcgccag gtatatttat cgacctgacc acggaagaac tggctgcggt tagtgccctt
     8581 ttcacggatg atctcggctc gttcgattaa cgctttatcg ttaatcagcg tcgcgccgcc
     8641 ttcaccaccc gccgtgtagt ttttggtttc atggaagcta aagcagccaa tatgaccaat
     8701 ggttcccagt gcacgccctt tgtaagtgga catcacgccc tgagcggcat cttccaccac
     8761 aaacaaatta tgctttttcg ccaacgccat aatggtgtcc atttcgcagg ccacacccgc
     8821 gtaatggacc ggcacgataa cgcgcgtttt gtcggtgatc gctgcttcaa tcaacgtttc
     8881 gtcgatgttc atggtgtccg ggcgaacatc cacaaagacg attttcgcgc cacgcagcac
     8941 aaaggcattg gcggtggaga caaaggtgta gctcggcatg atcacttcat cgccaggctg
     9001 gatatcgagc agcagcgccg ccatctccag cgaagcggtg caggacggcg tcagtaacac
     9061 tttggcgctg ccaaaacgtt gctccagcca ctgctggcag cgacgggtaa aaccgccatc
     9121 gccacacagt ttgccgctac ccattgccga ctgcatatag tcgagttcgg ttcccaccac
     9181 cggcggtgcg ttaaatggaa tcatgtgatc acctgtataa ccagtacgcg gtgctttcta
     9241 cattcgcacc actttgtatg tatcgtttaa gcgcggcggt gttgcccatt tgggtcgcca
     9301 cccgcaaagt tgttttaccg cgagcatacg cccagtttag cgccgtttgc atcagctcag
     9361 cacctgcacc gcgtccagcc agcaggccaa ttcgcgcatc tgtcgcattg agttcccgta
     9421 aagagacata gccgcgaata tcgccggacg ccgcacgtag aatcagacat tgatgatcaa
     9481 aggtgccgcg cacggcattt tcaatccact gtgcataaaa gcgaccgctg gcgtcaggcg
     9541 cataccacgg cgcacgaaaa cggctttgcg caaatgcggc gctagctaac tgacgtaatg
     9601 cgggaatatc ggtctcttgt gccacgacag caccgctatc actgacattg ttcacgggta
     9661 gcgccaaatc aacttcacct tctaccaggg agaatcccag ctgttgcagg gcatccagtt
     9721 cccccgtatt tgatgccgca attttggcct gcacccgtga ccatggcgct aaggcgtctg
     9781 acgtcaggag cggtgcttca gacgtaatgc gcacgatggc gctgttaaca ccaaagaagg
     9841 cgttttccca ggttagtggc tcaatactgg cgcggacggg cacgaagtaa ctccagcaga
     9901 tattggccgt agccagtttt cgctaatgaa ctggcagcac gcttcacacc ctcgtcatcg
     9961 agccagccgt tacgccaggc gatctcttcc aggcaggcaa tcttaaagcc ctggcgtttt
    10021 tctactgtct gcacaaaagt gctggcctca atcaggctgt cgtgagtgcc ggtatccagc
    10081 caggcaaatc cgcgcccgag cagttcaacg gtcaggttgc ccgcctcgag gtacatctgg
    10141 ttgatggagg taatctccag ttcaccgcgc tccgacggct tcacctgctt tgcgtactcc
    10201 acgactttac tgtcgtagaa ataaagcccg gtcaccgccc agtttgactt cggctgtttt
    10261 ggcttttctt caagcgagat agcgcggaaa ttgtcgtcaa attccaccac gccaaagcgt
    10321 tccgggtcca tcacctgata gccaaaaacc gtcgcccctt ccgtgcgcgc cgcaacatga
    10381 cgcagcttcg gactgaagcc ctgaccgaag aagatgttat cgcccagcac cagacaagaa
    10441 ggttcaccat taaggaaggt ttcaccgatg ataaaggcct gcgccagacc gtccgggctg
    10501 ggctgttcgg catattccag ctggataccg aactcactac catcgcccag caggcgctgg
    10561 aaataacctt tatcttccgg cgtagtgatg atgagaattt cgcggatacc ggccagcatc
    10621 agcaccgaca gcggatagta aatcattggc ttatcgtaaa tcggcaacag ttgcttcgat
    10681 acgccgcgcg taatcggatg caatcgggta ccggaaccgc ccgccaggat aatacccttc
    10741 atgtttgcct ccgctgaaaa ttagcctttc agacctaaac gctcaccctg atagctgccg
    10801 tcctgcacct gcttccacca gctttcatta gccagatacc actgaaccgt tttacgcatt
    10861 ccactttcaa aggtttcctg cggcagccag ccaagttcac gggcaatttt cgaagcatca
    10921 atggcataac gcagatcatg ccccggacgg tcagcgacaa aggtgatcag gtcacgatag
    10981 tacgccacgc cgtgcggctt gttcgaagcc agttcttcca gcagctcgca aatggtttcc
    11041 acaacatcga gattcttacg ctcgttgtga ccaccaatat tataggtttc accgactttc
    11101 ccggtggtcg ccacgcaata cagcgcgcgg gcgtgatctt ccacatacag ccagtcacgg
    11161 atttgctgcc cgttgccata taccggcagc ggtttacccg ccagcgcgtt gaggatcatc
    11221 agcgggatca gtttttccgg aaagtggtaa ggaccgtagt tattcgagca gttggtgatc
    11281 agcgtcggca gaccgtaggt ccgcagccag gcgcgcacca gatggtcgct gctggctttt
    11341 gacgcggaat aagggctgct cggcgcatac ggcgtggttt cggtgaagaa atcatccgtc
    11401 gagtgcaggt caccatatac ttcgtcggtg gagatatgat gaaaacggaa cgctgatttt
    11461 ttatcttccg tcagcgtatt ccagtaagcc cgcgccgctt caagcaatgt ataagtcccg
    11521 acaatgttgg tttcaataaa cgctgccggg ccgtcaatag aacggtcaac atggctttcg
    11581 gctgccagat gcatgacaca gtctggctga tgctcagtga atacgcgtgc cagttctgcc
    11641 cgatcgcaga tatcaacttt ctcaaaggca aagcgctcgc tttgcgcgac cggtgccagc
    11701 gacatcaggt ttccggcgta ggtcagctta tcgaccacta ccaccgcatc gctcgtttcg
    11761 ttgatgatat aacgcaccag cgccgagcca ataaacccgg caccacctgt tatcagaatt
    11821 tttctcatca gcgccagact cctttggcat cgacgacata ctgctgatgg acattgtcgc
    11881 cattgataac tttgaactga ctatgatcga ccagcatcac cagcacgtct gccgttgcca
    11941 gcgcctcgtc aagctgcgcc agagtacaaa gcccggtcag ttttttcggc aactggtgga
    12001 tgttaggctc aacaaccagc gtttcgccgc tgtgccactg ggcgatcagt tcagcgattt
    12061 ccatcgccgg gctttcgcgc aggtcatcaa tattcggttt aaacgccaga ccaaagcagg
    12121 cgattttcag ttcactggct cgtttatcgg tagcagcaag gcagtcggca accgccgctt
    12181 tcacctgatc gataacccag aacggtttgt gatcgttaac ttcgcgcgcg gtacggataa
    12241 gccgcgcctg ctgggggttc tgtgccacga taaaccacgg atcaacagca atgcagtgac
    12301 cgcccacgcc agggccaggc tgaagaatat taacgcgagg gtgacgattc gccaggcgaa
    12361 tcagttccca gacgttaatc ccctgatcgg cacaaatcag cgacaattca ttagcaaaag
    12421 cgatattcac atcgcggaag ctgttttcgg tgagcttaca catttccgcc gtccgcgagt
    12481 tagtgacgac acactcacct tcgaggaaaa ttttgtacaa ttcgctggcg cgggccgaac
    12541 aaaccggcgt cataccacca atcacgcgat cgtttttaat cagctcgacc ataacctggc
    12601 ccggtaatac acgttccggg cagtaagcaa tgttgacgtc cgcctgctcg cccacctgct
    12661 gtgggaaagt cagatccggg cgcatctctg ctaaccattc tgccatcttc tcggttgacc
    12721 ccaccggcga ggtggattca aggatcacca gcgcgccttt tttcagcact ggcgcaatgg
    12781 agcgagcagc cgattcaacg taggtcatat ctggctcatg atcgccttta aacggcgtag
    12841 gtacagcaat cagccaggca tccgcttcaa ctggcgtcgt gctcgctcgt aaaaaaccgc
    12901 cttctacggc agtttttact acgctcgcca aatcaggctc gacgatatgg atttcgccac
    12961 gattgatggt atcaaccgcg tgttggttga tatcgacacc aatcacctgt ttttgccgtg
    13021 aggcaaacgc tgctgctgta ggcagcccga tgtaacccag tccgataaca gaaatggtcg
    13081 caaaactcat agtgatatcc gattattttt taacgcttcc agaatgcgag agcatgcctg
    13141 accatcacca tacgggttat gggcgcggct catagcttga tattcgtttt cgtcttttaa
    13201 aagacgcgtc acttcctcga caattcgctg cttatccgtg cctaccagac gcaccgtacc
    13261 cgccgtcacc gcttccggac gctcagtggt atcgcgcatc accagcaccg gtttccccag
    13321 cgaaggcgct tcttcctgaa tgccgcctga gtcagtcaaa atcagccagg cgtggttcat
    13381 cagccagaca aacggtaaat actcctgggg atcgatcaga atgacatttt tcacatgccc
    13441 cagaatgcga ttgaccggct ctctgacgtt cgggttgaga tgcaccggat agacaatctg
    13501 gatgtcctgg tgcgtggtgg cgatgtctgc cagcgcgtgg cagatttctt caaagccacg
    13561 accgaaactc tcacgcctat gaccggtcac cagaatcatc tttttatcgg gggcgataaa
    13621 cgggtaattt gccgccagtt ctgaacgcag cgtgtcgctg ctcatcacct ggtcacgcac
    13681 ccataacagt gcatcaatga ctgtattacc ggtaatgaag attcggctat ccgcaacgtt
    13741 ttcacgcagc aagttttgcc gggaagtttc ggttggagag aagtgataca tcgccagatg
    13801 cccggtcaat gtacggttag cctcttccgg ccacggcgaa tagagagcgc ccgtgcgcag
    13861 accagcctca acgtgaccaa caggaatacg ctgataaaac gccgccaggc tggttgccag
    13921 cgtcgtcgtc gtatcgccgt gaaccagcac gacgtctggt ttgaactcgg caagaatagg
    13981 ttttagccct tccagaatcc gacaggttat ctctgtcagg ccctgtcctg gctgcattat
    14041 gttgagatcg tagtcaggta caatggaaaa gagtttcagt acctgatcaa gcatctcccg
    14101 atgctgcgca gtgacgcaaa ctttagcctc aaaaaaagga tcttttgcca acgcatgcac
    14161 caacggagcc atcttgatgg cttccgggcg cgtaccaaat acagtcagta ctttcacatc
    14221 gattctcttc gaataagcgg cgagcgcctt tgcgctcacc gcagcagtgt tgctatttcg
    14281 agcaacggcg ggttaatgcg acaccagccc cgatcagccc cccgacaatg ccccacataa
    14341 tcatcaggaa ggcacgacgt gggctatcgc gttttaccgg ttcttccggc gtacgcaaat
    14401 agcgataggt ctgaaaacgc ggatccaggg ttggaccaac attcagggtg tttaacatgg
    14461 ctcgattctg atcatagtcg agatcaaagg ccggaccgac ggcctgtaaa ttttccagtc
    14521 gagcctggag cattggacgc ccaagcagga acatttctga atcaggtaat tcctcggcag
    14581 gcacatctgt cgcactgcgc gaaatattat gctgctcagc aattttcagc gcctgctcaa
    14641 tactgttcat ccggcggtcg tagatggctt tcgccacctc ttcctgacgc ttcacctgag
    14701 ctttcatctg gatggtgcgt gccgcccatg cgcctttcag ctcatcattc agatggctgg
    14761 ctgcacgctg gctggcaaaa gcaacatact gacgtaacag gttattagcg tcaggcgcgg
    14821 tttcggcaat aagcttcacg ctgtcattga ccgcgcgagt aaagtctccg gggataaact
    14881 ggatgttgtt aatcatttca tccagcaacg ccgcatcggc tttgctgttg cccaccatcc
    14941 gctgtttgta atagtcggtt tgcagccaga actctctgcg ggtatcccac gaggccagtt
    15001 gcataacaaa ctctttatag gcttcgtcca tgaccgatgg ttgatcggca gaagccatgt
    15061 ttgaacggac atccaggtta cgcaaaaatt gctgctgcga gtaatatccc cccagcatat
    15121 tcaccgttgg acgatcggta atcgccgtcg agctccactc ctgacgagca aaaaaagtat
    15181 acgccagcgc gattaacgca aacgccagcc ccatgccaat aatccatagc ttcccagccc
    15241 acaaggtacg aaacaaccca cgaatatcca gttcattttc agcgtcttcg gccggtttcc
    15301 caggcattgg ttgtgtcatc acatcctcat ttatttggtt aaattggggc tgccaccacg
    15361 atttctacgc agtctgcgtt ttacgcgctt aataaagcga gcaactttcc aggcacgctt
    15421 aatgcaatat ccatagagga agaatgctag caaaaagagc accagcatga cccactccgg
    15481 gacaaaatga gaatattctg ccagcacgcc aatggaagcg agcagtgctg cggcaagggt
    15541 aatcagcaca aacgcctgac gggaagtaaa cccggcacgc atgatcaaat ggtgaatatg
    15601 ctgacggtca ggagagaatg ggctcatgcc tttacgcagg cgacggtaca taatcgccac
    15661 catatccatt agcggaatgg cgattatcca caaagcggta accgggctga tgggatgggt
    15721 tttgccctgg gtcgtttcga gcaggatcca gataacggta aaaccaatca gcgtactgcc
    15781 cgcatcaccc ataaagactt tgtagcggcg acccaggata ccaaggttaa gcatgatgta
    15841 tggcaggatg gcggcgatca tcgcaaagca ccagattgcg aggctggttt gcccgtcgaa
    15901 ccacaaaatc ataccgattg ctgcaaacga gacgcaggac aacccgccca gcaagccatc
    15961 aatgccatca accatgttga acgcattaat ggccgcccag acggcaaata gcgtcaggaa
    16021 gtaaccaaac ggtccgagca ccatctccca ggagccaaag atataaccca ggctactgag
    16081 ataaagtttg ccgaacacca tcataacaat gccaacagcg gcctgtatgg tggcacggat
    16141 ttttacgctg atatcaaaac gatcatccag cgcgccaatg aaaacaagca caccggcaca
    16201 agcgagatag agagatgcat gcggaatata gtaatcgaca attccgaacg tgaagcaaat
    16261 ccctgcgtaa accgaaatcc ccccaacgag aggtatcaat ccctggtgac gtttgcggaa
    16321 gtttggttta tccactaaac cgactttttt tgccacctta cgggcaaaaa acagaaacag
    16381 tgtcgtgaat aaaaaaatac tgatgagatc agtactcact gtcagtaaat tcacaatgca
    16441 tgctctcaga gaaaattatt agcagaagta taaccacgaa gacctttatt cagaagggaa
    16501 agtccgttcc gaacccacca gccctgtgca aaaatcaacc atttgatatg caaattgcat
    16561 aacatgccag caaattccag gaattttcct aacatcgcca gcgcgacatt aagattacag
    16621 atataaaa
//
LOCUS       DAJTSI010000089        16389 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000089 DAJTSI010000000
VERSION     DAJTSI010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.089"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(87..2063)
                     /gene="speA"
                     /locus_tag="OO327_002601"
     CDS             complement(87..2063)
                     /gene="speA"
                     /locus_tag="OO327_002601"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR6019095.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="OO327_002602"
     CDS             complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="OO327_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR6019096.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2339..2554
                     /locus_tag="OO327_002603"
     CDS             2339..2554
                     /locus_tag="OO327_002603"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019097.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2858..4012
                     /gene="metK"
                     /locus_tag="OO327_002604"
     CDS             2858..4012
                     /gene="metK"
                     /locus_tag="OO327_002604"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR6019098.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4449..5843
                     /gene="galP"
                     /locus_tag="OO327_002605"
     CDS             4449..5843
                     /gene="galP"
                     /locus_tag="OO327_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR6019099.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5920..6417
                     /locus_tag="OO327_002606"
     CDS             5920..6417
                     /locus_tag="OO327_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR6019100.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6512..7219
                     /gene="endA"
                     /locus_tag="OO327_002607"
     CDS             6512..7219
                     /gene="endA"
                     /locus_tag="OO327_002607"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR6019101.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7299..8030
                     /gene="rsmE"
                     /locus_tag="OO327_002608"
     CDS             7299..8030
                     /gene="rsmE"
                     /locus_tag="OO327_002608"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR6019102.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8043..8993
                     /gene="gshB"
                     /locus_tag="OO327_002609"
     CDS             8043..8993
                     /gene="gshB"
                     /locus_tag="OO327_002609"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR6019103.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9102..9665
                     /locus_tag="OO327_002610"
     CDS             9102..9665
                     /locus_tag="OO327_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR6019104.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9665..10081
                     /gene="ruvX"
                     /locus_tag="OO327_002611"
     CDS             9665..10081
                     /gene="ruvX"
                     /locus_tag="OO327_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR6019105.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10257..11237)
                     /locus_tag="OO327_002612"
     CDS             complement(10257..11237)
                     /locus_tag="OO327_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR6019106.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQRVGEGRL"
     gene            11255..11959
                     /gene="yggS"
                     /locus_tag="OO327_002613"
     CDS             11255..11959
                     /gene="yggS"
                     /locus_tag="OO327_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR6019107.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11977..12543
                     /gene="yggT"
                     /locus_tag="OO327_002614"
     CDS             11977..12543
                     /gene="yggT"
                     /locus_tag="OO327_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR6019108.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12540..12830
                     /locus_tag="OO327_002615"
     CDS             12540..12830
                     /locus_tag="OO327_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR6019109.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12838..13431
                     /locus_tag="OO327_002616"
     CDS             12838..13431
                     /locus_tag="OO327_002616"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR6019110.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13424..14560
                     /gene="hemW"
                     /locus_tag="OO327_002617"
     CDS             13424..14560
                     /gene="hemW"
                     /locus_tag="OO327_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR6019111.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14874..15837
                     /locus_tag="OO327_002618"
                     /pseudo
     CDS             14874..15837
                     /locus_tag="OO327_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15882..16385
                     /locus_tag="OO327_002619"
     CDS             15882..16385
                     /locus_tag="OO327_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR6019112.1"
                     /translation="MIFNRLKLAVDRVIATFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
BASE COUNT         4059 a         4220 c         4123 g         3987 t
ORIGIN      
        1 agcagctaga ttatttttaa gaatatataa aaagaaagcg ggccgattta cgtaaataaa
       61 tcgacccgcc agcggaataa tcttaattat tcatcttcaa gataagtata accgtacaaa
      121 cctgcctcga actcttcaag gaactgttgt tgcagttcag catcaagatc ggttttcttc
      181 acttgatcgc ggaactgggt taacagcgtt ttcggatcga gctgtacata ttgcagcatg
      241 tccgccacgg tatcgccttc gtcagacagt tctacttcta cgctaccgtc agggaacacg
      301 aacacgtcaa ctgcttcggt atcaccgaac aggttgtgca tattgccaag gatctcctga
      361 tatgcaccga ccataaagaa accgagcatc ggcggattct ctggatcgta ctccggcatt
      421 ggcattgtcg tggcaatacc atcaccatca atatagtggt cgatagcacc gtcagagtca
      481 caggtaatat ccagcagcac agcgcgacgt tccggcactt gatccagccc ttccagcggt
      541 agaaccggga acaactggtc gatcccccat gcgtccggca ttgactggaa cagcgagaaa
      601 ttgacgtaca ttttgtccgc catacgttcc tgcagttcgt cgattatcgg acgatgcgca
      661 cggttttgcg gatccagttg cttctgcact tcatggcaca tgctcagata gagttgttct
      721 gcccatgcac gttcttgcag gctaaaggtg ccggaagagt agccgatatg aatgtcgtgc
      781 agatccatct ggctgtcgtg taaccattca cgcaaagaac ggcgagttcc cggttcgtgc
      841 atttcctgcc aggtttccca catgctttgc agcgcacgcg gcgcatcttc tacgggcgcg
      901 gtcggcaccg tatattcgtt acgttccacg ccgatgatat tcgacaccag cacggtatgg
      961 tgcgcagtca ccgcacggcc cgattcggtg attaccgtcg gatgcggcag accattttct
     1021 tcacacgcat cgccaatcgc ccagatgatg ttgttggcgt attcattgag gccatagttt
     1081 acggagcagt cagactgcga acgggtgcct tcataatcca cgcccagacc gccgccgacg
     1141 tcgaagcact gaatattgac gcccagcttg tgcagttcca catagaaacg cgcggattca
     1201 cgaacgcctg tcgcgatatc gcgaatattc gccatctgcg aaccgaggtg gaagtgcagt
     1261 agttgcaggc tgtcgagacg cccggcttca cgcagggttt caaccagttg cagtacctga
     1321 gtcgcagcca ggccgaactt cgatttttcc ccgccggagg actgccattt acccgagccc
     1381 tgcgaagcca gacgtgcacg cacgcccaga cgaggaacga cattcagacg ttctgcttca
     1441 tccagcacaa tggcgatttc tgacatcttc tcaatgacca gatagacctt gtgccccatc
     1501 ttctcgccaa ttaatgccag gcggatatat tcgcggtctt tataaccgtt gcagacgatg
     1561 acgctacgag tcatgccagc atgtgccagc actgccatca actcggcttt ggaaccggct
     1621 tccagaccca gcggttcacc cgaatgaatc agggactcga tcacgcggcg gtgctggtta
     1681 actttgatcg gataaacaag gaagtaatcg ccgttatagc cgtaggattc cctcgcacgt
     1741 ttgaacgcgg cgttaatgga acgcaaacgg tgctgcagga tctgtgggaa acagaacagt
     1801 gcaggcagac gctgaccctg tgcttcacga gttttcacta actgcgcgag atcgacgcga
     1861 gcttccggga cgtccgggtc cgggcacacg ctgatgtggc ccagctcgtt aacgtcatag
     1921 tagttattgc cccaccaggc aatattgtaa gtacgcagca tcttgctggc ttcctgggag
     1981 ctcattgcaa cctcctgcat ggagcgtagt acaccgtgtt cgcccgctga cgaaggcaaa
     2041 cccatagaca tgtcgtcaga catagcgaac ctcaaattat tttattaagt gtaaaacagt
     2101 taacgactgt cgcagcctgg aactgcgata acaacccata agcacatgga ttttccagca
     2161 gtgtatgctg acgctccgac tgcgcaaccg gtttcttttt cataacatta ttaagcacat
     2221 aaccgaacgt aagtgtgaca gttcggcgaa accacgagaa aactcttgtt tttacaagag
     2281 cgcccttgtt cagtcctcag taactgtaac cagctcttga atcctgagaa gcgccgagat
     2341 gggtataaca tcggcaggta tgcaaagcag agatgcagag tgcggggaac gagtcttcac
     2401 cagaacggtg agacaggtta agcagcagac aacggttcat tatttcgtat cacctccacg
     2461 gccgcctgtt aagacgaacc cacaagccaa aactctgatt tcaacccggc tggaagtggc
     2521 aacacgaaag aaacgtcgtg tgctttttat ttaagccgcg cgccgcgttt tataccccac
     2581 aatggcagaa aattgcaaaa gataaatacg cagaatgccg gcattgtcag gaaaaatttc
     2641 cagccacgtt tttaacagaa tgagacacga ttcaaaaaaa agtggaaatc gggtgaagaa
     2701 ttgacctaaa atagccatcc agatgttaat ccatccatac cgattaacac tcagactgcc
     2761 agtgttttta acctgcagag tcgtggtagg atccgctacc acagaaaatc cacacaacag
     2821 tttgagctaa ccaaattctc tttaggtgat attaaatatg gcaaaacacc tttttacgtc
     2881 cgagtccgtc tctgaagggc atcctgacaa aattgctgac caaatctctg atgccgtttt
     2941 agacgcgatc ctcgaacagg atccgaaagc acgcgttgct tgcgaaacct acgtaaaaac
     3001 cggcatggtt ttagttggcg gcgaaatcac caccagcgcc tgggtagaca tcgaagagat
     3061 cacccgtaac accgttcgcg aaattggcta tgtgcattcc gacatgggct ttgacgctaa
     3121 ctcctgtgcg gttctgagcg ctatcggcaa acagtctcct gacatcaacc aaggcgttga
     3181 ccgtgccgat ccgctggaac agggcgcggg tgaccagggt ctgatgtttg gctacgcaac
     3241 taatgaaacc gacgtgctga tgccagcacc tatcacctat gcacaccgtc tggtacagcg
     3301 tcaggctgaa gtgcgtaaaa acggcactct gccgtggctg cgcccggacg cgaaaagcca
     3361 ggtgactttc cagtatgacg acggcaaaat cgttggtatt gatgctgtcg tgctttccac
     3421 tcagcactct gaagagatcg accagaaatc gctgcaagaa gcggtaatgg aagagatcat
     3481 caagccaatt ctgcccgctg aatggctgac ttctgccacc aaattcttca tcaacccgac
     3541 cggtcgtttc gttatcggtg gcccaatggg tgactgcggt ctgactggcc gtaaaattat
     3601 cgttgatacc tacggcggca tggcgcgtca cggtggcggt gcattctctg gtaaagatcc
     3661 atcaaaagtg gaccgttccg cagcctacgc agcacgttat gtcgcgaaaa acatcgttgc
     3721 tgctggcctg gccgatcgtt gtgaaattca ggtttcctac gcaatcggcg tggctgaacc
     3781 gacctccatc atggtagaaa ctttcggtac tgagaaagtg ccttctgaac aactgactct
     3841 gctggtacgt gagttcttcg acctgcgccc atacggactg attcagatgc tggatctgct
     3901 gcacccgatc tacaaagaaa ccgcagcata cggtcacttt ggtcgtgaac atttcccgtg
     3961 ggaaaaaacc gacaaagcgc agctgctgcg cgatgctgcc ggtctgaagt aatctttctt
     4021 cacctgcgtt caaaggccag cctcgcgctg gcctttttct tttacctcac cgcggtggat
     4081 aggcgttcac gccgcatccg gcaaaaaaac tgcccgcaca ataacatcat tcttcctgat
     4141 cacgtttcac ggcaaattac catcacaact gaaaccgatt acaccaacca caccagcaaa
     4201 atatttgtaa tattttcata ttattattca gctttcacag ttgttacatt tcttttcagt
     4261 aaagtcttaa ttgcagatga cagcgtttaa tctatgatga tataactcaa ttattttcat
     4321 gcccttaaat cataactaag ataaatgtta gtgtaagcga ttacactgat gtgatttgct
     4381 tcacatcttt ttacgtcgta ctcacctatc ttaattcaca ataaaaaata actatattgg
     4441 agggcatcat gcctgacgct aaaaaacagg ggcggtcaaa caaggcaatg acgtttttcg
     4501 tctgcttcct tgccgctctg gcgggattac tctttggcct ggatatcggt gtaattgctg
     4561 gcgcactgcc gtttattgca gatgaattcc agataacttc gcacacgcaa gaatgggtcg
     4621 taagctccat gatgttcggt gcggcagtcg gtgcggtggg catcggctgg ctctccttta
     4681 aactcgggcg caaaaagagc ctgatgatcg gcgcaatcct gtttgttgcc ggttcactgt
     4741 tctctgcggc tgcgccaaac gttgaagtac tgattctttc gcgcgttctg ctggggctgg
     4801 cggtgggtgt ggcctcttat accgcaccac tgtacctgtc agaaattgcg ccggaaaaaa
     4861 ttcgcggcag tatgatctcg atgtatcagt tgatgatcac tatcgggatc ctcggtgctt
     4921 atctttctga taccgccttc agctacaccg gtgcatggcg ctggatgctg ggtgtgatta
     4981 tcatcccggc aattttgctg ctgattggtg tcttcttcct gccagacagc ccacgttggt
     5041 ttgccgccaa acgccgtttt gttgatgccg aacgcgtgct gctacgcctg cgtgacacca
     5101 gcgcggaagc gaaacgcgaa ctggatgaaa tccgtgaaag tttgcaggtt aaacagagtg
     5161 gctgggcgct gtttaaagag aatagcaact tccgccgcgc ggtgttcctt ggcgtactgt
     5221 tacaggtaat gcagcaattc accgggatga acgtcatcat gtattacgcg ccgaaaatct
     5281 tcgaactggc gggttatacc aacaccaccg agcaaatgtg ggggacagtg attgtcggcc
     5341 tgaccaacgt acttgccacc tttatcgcaa tcggccttgt tgaccgctgg ggacgtaaac
     5401 caacgctaac gctgggcttc ctggtgatgg ctgctggcat gggcgtactc ggtacaatga
     5461 tgcatatcgg tatccactct ccgtcggcgc agtatttcgc catcgccatg ctgctgatgt
     5521 ttattgtcgg ttttgccatg agtgccggtc cgctgatttg ggtactgtgc tccgaaattc
     5581 agccgctgaa aggccgcgat tttggcatca cctgctccac cgccaccaac tggattgcca
     5641 acatgatcgt tggcgcaacg ttcctgacca tgctcaacac gctgggtaac gccaacacct
     5701 tctgggtgta tgcggctctg aacgtactgt ttatcctgct gacattgtgg ctggtaccgg
     5761 aaaccaaaca cgtttcgctg gaacatattg aacgtaatct gatgaaaggt cgtaaactgc
     5821 gcgaaatcgg cgctcacgat taatctcctc aggcttcctc ccgtcgcgga ggaagccgcc
     5881 tcttgcagtc atcttttctt cgctctatcc tctgccgcta tgaaaacatc ccgtctccct
     5941 atcgccatcc aacaggccgt tatgcgccgc ctgcgggaaa aactcgccca ggccaacctg
     6001 aagctagggc gtaactaccc ggagccaaaa ctctcttaca cccagcgcgg aacctccgcc
     6061 ggaacagcct ggctggaaag ctatgaaatc cgcctcaatc ccgttttgct gttggaaaac
     6121 agtgaagctt ttattgaaga agtggtaccg cacgaactgg cacatttgct ggtatggaaa
     6181 catttcggcc gcgtagcgcc acatggcaaa gagtggaagt ggatgatgga aagcgtgctg
     6241 ggcgttcccg cccgtcgtac gcatcagttc gaactgcaat ccgtgcgtcg caacaccttc
     6301 ccctaccgct gtaagtgcca ggaacatcag cttaccgtac gccgccataa tcgcgtagtt
     6361 cgtggcgagg ccatctatcg ctgtgttcac tgcggtgaac agctggttgc gaaataacca
     6421 cctgaactat caggaacttt cctgatctgg ctgattgcat accaaaacaa ctttcgctac
     6481 gttgctggct cgttttaaca cggagtaagt gatgtaccgt tatttgtcta ttgctgcggt
     6541 ggtactgagc gcagcatttt ccggcccgac gttggccgaa ggtatcaata gtttttctca
     6601 ggcgaaagcc gcggcggtaa aagtccacgc tgatgcgccc ggtacgtttt attgcggatg
     6661 taaaattaac tggcagggca aaaaaggcgt tgttgatctg caatcgtgcg gctatcaggt
     6721 gcgcaaaaat gaaaaccgcg ccagccgcgt agagtgggaa cacgtcgttc ccgcctggca
     6781 attcggtcac cagcgccagt gctggcagga cggtggacgt aaaaactgcg ctaaagatcc
     6841 ggtctatcgc aagatggaaa gcgatatgca taacctgcag ccgtcagtcg gcgaggtgaa
     6901 tggcgatcgc ggcaatttta tgtacagcca gtggaatggc ggcgaaggcc agtacggtca
     6961 atgcgccatg aaggtcgatt tcaaagaaaa agctgccgaa ccaccagcgc gtgcacgcgg
     7021 tgccattgcg cgcacctact tctatatgcg cgaccaatac aacctgacac tctctcgcca
     7081 gcaaacgcag ctgttcaacg catggaacaa gatgtatccg gttaccgact gggagtgcga
     7141 gcgcgatgaa cgcatcgcga aggtgcaggg caatcataac ccgtatgtgc aacgcgcttg
     7201 ccaggcgcga aagagctaac ctacactagc gggattcttt ttgttaaccc ctaccccacg
     7261 cgtacaaccg cgtggggaga cgacgcggat ttttaactat gcgtatcccc cgcatttatc
     7321 atcctgaacc actgaccagc cattctcaca tcgcgctttg cgaagatgcc gccaaccata
     7381 tcgggcgcgt actgcgcatg gggccggggc aggcgttgca attgtttgac ggtagcaacc
     7441 aggtctttga cgccgaaatt accagcgcca gcaaaaaaag cgtggaagtg aaggtgctgg
     7501 aaggccagat cgacgatcgc gaatctccgc tgcatattca cctcggtcag gtgatgtcgc
     7561 gtggtgaaaa aatggaattt actatccaga aatcgatcga actcggtgta agcctcatta
     7621 cgccactttt ttctgagcgc tgcggcgtta aactggatag tgaacgtctg aacaagaagc
     7681 ttcagcagtg gcagaagatt gcaattgctg cctgtgagca gtgtggtcgt aaccgggtgc
     7741 cggaaatccg tccagcgatg gatctggaag cctggtgtgc agagcaggat gaaggactga
     7801 aactgaatct tcacccgcgc gccagtaaca gcatcaatac gttgccttta ccggttgaac
     7861 gcgtccgcct gctgattggc ccggaaggcg gtttatcggc agatgaaatt gccatgactg
     7921 cccgctatca atttactgat atcctgttgg gacctcgcgt tttgcgtaca gagacaactg
     7981 cgctcaccgc cattaccgcg ctacaagtac gatttggcga tttgggctaa cggagaagaa
     8041 taatgatcaa gctcggcatc gtgatggacc ccatcgcaaa catcaacatc aagaaagatt
     8101 ccagtttcgc tatgttgctg gaagcacagc gtcgtggtta cgaacttcac tatatggaga
     8161 tgggcgatct gtatctgatc aatggcgaag cccgcgccca tacccgcacg ctgaacgtga
     8221 agcagaacta cgaagagtgg ttttcgttcg tcggtgaaca ggatctgccg ctggccgatc
     8281 tcgatgtgat cctgatgcgt aaagacccgc cgtttgatac cgagtttatc tacgcgacct
     8341 atattctgga acgtgccgaa gagaaaggga cgctgatcgt taacaagccg cagagcctgc
     8401 gcgactgtaa cgagaaactg tttaccgcct ggttctctga cttaacgcca gaaacgctgg
     8461 ttacgcgcaa taaagcgcag ctaaaagcgt tctgggagaa acacagcgac atcattctta
     8521 agccgctgga cggtatgggc ggcgcgtcga ttttccgcgt gaaagaaggc gatccaaacc
     8581 tcggcgtgat tgccgaaacc ctgactgagc atggcacttg ctactgcatg gcgcaaaatt
     8641 acctgccagc cattaaagat ggcgacaaac gcgtgctggt ggtggatggc gagccggtac
     8701 cgtactgcct ggcgcgtatt ccgcaggggg gcgaaacccg tggcaatctg gctgccggtg
     8761 gtcgcggtga acctcgtccg ctgacggaaa gtgactggaa aatcgcccgt cagatcgggc
     8821 cgacgctgaa agaaaaaggg ctgatttttg ttggtctgga tatcatcggc gaccgtctga
     8881 ctgaaattaa cgtcaccagc ccaacctgta ttcgtgagat tgaagcagag tttccggtgt
     8941 cgatcaccgg aatgttaatg gatgccatcg aagcacgttt acagcagcag taacccacct
     9001 tagcgagaag gatctcgttg agactctgag tgacagcgcc cttctttcca cgcatactgg
     9061 gcgctgttgc ttttttgaac caggaaacag aacctctgac aatgaattta cagcatcact
     9121 ttcttattgc catgcctgct ctccaggatc cgattttccg tcgttccgtg gtctacattt
     9181 gcgaacataa taccaatggt gcaatgggga tcatcgtcaa caagccgctg gaaaatctca
     9241 aaattgaagg gattctggaa aagctgaaga tcacgccgga gccgcgtgat gaatcaatcc
     9301 gtctggataa accggttatg ctcggcggcc cactggctga agatcgcggg tttattttgc
     9361 atactccgcc ctccaatttt gcttccagca ttcgcatttc agacaacacg gtgatgacca
     9421 cttcccgcga tgtgctggaa acgctcggca ccgataaaca accgtctgac gtattggtgg
     9481 ctctgggcta tgcctcctgg gaaaaaggtc aactggaaca agaaattctc gataacgcgt
     9541 ggctaacggc cccggcggat ctgaatattc tgttcaaaac gccgattgcc gaccgctggc
     9601 gcgaggcggc aaaactgatt ggtgtggata ttctcaccat gcctggtgtg gcaggacacg
     9661 cctgatgagt ggaaccttac tcgccttcga cttcggcacc aaaagcattg gcgtagcggt
     9721 cggccaacgc attaccggca ccgctcgccc tttgcctgca attaaagcgc aggacggtac
     9781 gccggactgg aacctcatcg aacgtttact gaaagagtgg cagcctgacg aaatcatcgt
     9841 cggtttgccg ctgaatatgg acggcaccga acagccgtta accgccagag cgcgcaaatt
     9901 tgccaaccgt attcatggtc gtttcggtgt tgaagtaaag ctccatgacg agcgtcttag
     9961 cactgtggaa gcccgttccg gtctttttga acagggcggc tatcgggcgc tcaacaaagg
    10021 caaagttgac tctgcctctg cggttattat tctcgaaagc tatttcgagc agggatatta
    10081 aggcgatcta aaagcctggc ggatagtcgt taccgccatc ttgccggatg cggcgtgaac
    10141 gccttatcca gcacacatct ggcagcggct gtaggtctga taagacgcgt tagcgtcgca
    10201 tcagacattt attgccttta taggccctat aaacttgcgc atcgggcatg gcaacgtcac
    10261 aaacgccctt cccccacccg ctgctgataa ctctgctgaa acgttaacat ccccaccttc
    10321 tgcccggttt gaataacatg cggtaactgg tgggttttcc cttcgcgaat caaattcccc
    10381 accgcgggag tgttaatcag taattcaaac agcgccacgc gtccttcctg tttatccact
    10441 tccagctttt gtgacagcac tgcccgtaaa ctacccgcaa gttgattacg tacgggatct
    10501 ttttcctgcg ccggaaatga atccaccagt cgctcaactg cctgcgcggc accacgtgta
    10561 tgtaaggttg ccagcaccag atgcccggtt tctgccgccg tcagcgccag acggattgtc
    10621 tcactgtcac gtagctctcc gagcaaaatc acatcaggat cttcccgcaa tgcagcccgc
    10681 aaccccgatg cgaacgtcat acagtgcaaa ccaatttccc gctgctggat caaacatcgc
    10741 tggctggcat agagatattc cacaggatct tccagcgtca gaatatgcgc atcggcatgt
    10801 tgattaagat agccaaccat cgccgccagc gtggtagatt tgccactccc cgtagccccc
    10861 gtcaccagaa tcaggccatt ctcgctcttg agtaattccg gcaataccgg tggtgcacca
    10921 agctgttcga gctgtggaca gtgcgaaggt aacaaccgta atgccagaga aataccttgc
    10981 cgttgcgcga acgcactgcc acgcaaccgc tggttttccg ccagcgacac ggcaaaatcc
    11041 agctggccat tttccagcaa tattgcccgc tgatcgtcat ccagccactc ccgcagtagc
    11101 tcttcgacgt ccggcgcatc aaacggcgca gcttccattc tgccacgaat gcgccatcgt
    11161 gcgggccagg cgctgcacag gtgtagatcc gagacgttat gctttacact aagggccaca
    11221 atttcttcca tattcatact aagatcctcg gaaaatgaac gatattgcgc ataacctggc
    11281 acaggtccgg gacaaaatct cagcggctgc aacgcgttgc ggccgttctc cagaagaaat
    11341 tacgctgctt gcagtcagta aaacaaaacc tgcgagcgcc atcgcagaag ccattgatgc
    11401 cgggcagcgt caatttggtg aaaactacgt tcaggaaggg gtagataaaa ttcgccactt
    11461 tcaggaactg ggcgtaacag gattagaatg gcattttatt ggcccgttgc agtctaataa
    11521 aagccgccta gtggcagagc atttcgactg gtgtcatacc atcgaccgtt tgcgcattgc
    11581 tacccgcctc aacgagcagc gcccggcaga acttccccct cttaacgtac tgattcaaat
    11641 taatattagt gatgaaaaca gtaagtccgg gattcaactg gcagaactgg acgagctggc
    11701 agcggcggtc gctgaactac cgcgtttacg tctgcgcggg ctgatggcaa tccctgcgct
    11761 tgagtcagaa tatgtaaggc agtttgaagt tgcacgccaa atggctgtag catttgccgg
    11821 actgaaaacg cgctacccgc atatcgacac gctctctctg ggaatgtcgg acgatatgga
    11881 agccgccatt gcggcaggta gcacgatggt tcgtatcggc actgcaattt ttggtgcgcg
    11941 tgattactct aaaaaataag gaattaaagg aacgccatga atacgttgac tttcctgctt
    12001 tcaacggtca ttgagctgta tacaatggtg ctgttattac gcatctggat gcagtgtgct
    12061 cattgtgatt tttacacccc cttctcacag tttgtagtga aggtagcgca gccaattatc
    12121 gggccactgc gccgcgttat tccggcaatg ggtcccattg acagcgcctc gctgctggtt
    12181 gcctatattc tcagttttat caaagccatt gtgctgtttg aagtggtgac cttcctgcca
    12241 atcatctgga ttgccggttt actgattctg ctgaaaacca tcggcttgct gattttctgg
    12301 gtcctgctgg tgatggcgat tatgagctgg gtaagccagg gtcgtagccc gattgaatac
    12361 gtgctgattc agctggccga tccgctgctg cgcccgattc gccgcttgct accggcaatg
    12421 ggtgggattg atttctcgcc gatgatcctc gttctgctgc tgtatgccat caatatgggt
    12481 gtcgcagaag tattacaggc aaccggaaac atgctgctgc cggggctgtg gatggcgtta
    12541 tgagtgccgt aacagttaat gatgacggtc tggttttacg gctctatatt cagccgaaag
    12601 ccagccgtga ttctattgtc ggtttacatg gcgacgaagt taaagtcgcc attaccgcgc
    12661 cgccggttga cggccaggcc aacagtcatc tggtgaagtt tctcggtaag caattccggg
    12721 ttgccaaaag ccaggtggtg attgaaaaag gcgaacttgg ccgccacaaa caaattaaaa
    12781 tcattaatcc gcaacaaatc ccgccagaaa tcgcggcgtt aattaattag gtatcctatg
    12841 caaaaagttg tcctcgcaac cggcaatgcc ggtaaagtgc gtgagctggc gtcgctgctt
    12901 agcgactttg gtcttgatat cgtggcccaa acagacctcg gcgttgattc cgctgaagaa
    12961 accggcctaa cctttatcga aaacgcgatt ctgaaagcgc gccatgcggc aaaagtgacc
    13021 ggtttaccgg caattgccga cgactctggt ctggcggtag atgcgctggg tggcgcgccg
    13081 ggtatttact ccgcgcgtta ttccggtgaa gacgcgaccg atcaaaagaa tctgcaaaaa
    13141 ctgttggaaa cactgaaaga cgtaccggac gaccaacgtc aggcgcgttt ccactgcgtg
    13201 ctggtatatc tgcgtcacgc ggaagatccg actccgctgg tatgccacgg cagctggccg
    13261 ggcgtgatta ctcgcgaacc agcgggcact ggtggctttg gttatgatcc aatcttcttc
    13321 gtaccttccg aagggaaaac cgccgccgaa ctgacccgcg aagaaaagag cgccatttcc
    13381 caccgtggtc aggcgttgaa actgctgctg gacgctttac gtaatggtta aattaccgcc
    13441 gctgagtctc tacattcaca tcccgtggtg cgtgcagaaa tgcccgtact gcgatttcaa
    13501 ctctcacgcg ttgaaaggag aagtgccgca cgacgattat gttcagcatc tgctttgcga
    13561 tctggacaat gatgtggctt acgctcaggg ccgtgaagta aagacaatct ttattggcgg
    13621 tggtacgccg agcctgcttt ccggcccggc gatgcaaacg ctgctagacg gcgtgcgtgc
    13681 gcgtttgccg ctggcagcgg atgcagaaat tactatggaa gcgaacccag gcacggtaga
    13741 agcggatcgc tttgtcgatt atcagcgtgc tggtgtaaac cgcatctcta ttggtgtgca
    13801 gagttttagc gaagaaaagc tgaaacgact tgggcgtatt catggcccgc aagaagcgaa
    13861 acgagcggca aagctggcga gcggtttagg gttacgtagc tttaaccttg atttgatgca
    13921 tggactaccg gatcaatcac tggaagaggc gcttggcgat ctacgccagg ccattgaact
    13981 gaatccgccg catctttcct ggtatcaact gactatcgaa cctaatacgc tgtttggctc
    14041 gcgaccgccg gtgctgccgg acgatgacgc gctgtgggat attttcgaac aggggcatca
    14101 gttattaacc gcagcgggtt atcagcaata tgaaacttcc gcttacgcca aacccggtta
    14161 tcagtgccag cacaatctca actactggcg ctttggtgac tacatcggta ttggctgcgg
    14221 cgcgcacggc aaagtgacct tcccggatgg gcgcattctg cgtaccacta aaacgcgtca
    14281 tcctcgtggt tttatgcaag gaaggtatct ggaaagccag cgtgatgtcg aagccgcaga
    14341 taagccgttt gagttcttta tgaatcgctt ccgtctgctg gaggccgcgc cgcgcgtgga
    14401 gtttattgcg tataccgggc tttgcgaaga tgtgattcgc ccacagttag acgaggcgat
    14461 tgcccagggt tatctcaccg aatgtgcgga ttactggcag ataacggaac atggtaaatt
    14521 attcttaaac tcacttcttg aattattcct cgctgagtaa tcattaatgc gcctgcgggc
    14581 gcattaatca cttcttttat ccccccgtta attaacctcg gcagaaatat atttcttact
    14641 gaataaatat cgacgacatt tattatatat atttagtgaa tatttactaa tcattcattg
    14701 attataaata tgtacaacct ggcgcacaaa tttaatattt aatttctgac tttatttcaa
    14761 gttgtgataa acatagacaa gttgtcagac atgttaacca atttgtcgca caaatcactg
    14821 ataagcacaa ttagatatca accaatttcc atccttcaac agggagtttt tttatgaaaa
    14881 tgaggtcatt tacccgctca ttagtctgcg catccttgct ggctctggtt agcactggcg
    14941 taaacgccgc agaaaaagtg gcgttaaagt tagcacacaa cctggaaaga agtcatgttg
    15001 tacatcagag ttttgaagag ctggcgaaag aggtaaaaca gctgtcaaaa ggtaatatgg
    15061 tgatccgtat ttatcccagc agccagatgg gtaatgcgcg agaaactatg gaattgttgc
    15121 aaaatggcgc actggatatg accaaaggtt ccgccagtga tctggagtct tttgataata
    15181 tttatgctat ctataatttg ccgttcttat taagttgttt ttggtgaagt tggtaaagaa
    15241 attatggact caacaaaaga caaaggcttt tttgctttat cagcttatgt agcgggtact
    15301 cgcagtttct atgcgaaaaa accgattaca aagccagaag atcttaaagg actgaaaatt
    15361 cgtgttcagc caagtccgac caccatcaaa atgattgaac tgatgggtgg ttcacccact
    15421 ccaatttcat ttggcgaagt ttatactgcg atgcagcaag gtgtagtaga tggcgcagaa
    15481 aataacgtgc cttcctggat gcagactcga catattgaaa ttgccaaagt tttttctgaa
    15541 gatgaacacg cctcaattcc tgactttttg gttatctcca ccaaaacatg gaataaatta
    15601 accccagaac agcaacaaat cctcgaaaca gcggcgaaga aatccgaagc ttatcaacaa
    15661 aaattgtggg aaaaaattga cgctgacaca cgtgctcagg cgaaagctat ggggggtgaa
    15721 attgtaaaag tggataaagc gccattccgt aaagccgtgc aacctctgtt tgacgacttc
    15781 aaaaaagatc caaaacaagc tgctttgtta gagaaattcg acaacgctgc tcaataatat
    15841 tccgggccag cctctggccc tgattttcca ggatgcttac aatgatattt aaccgcctga
    15901 aacttgcggt agaccgcgtc attgccacgt tttctgtcgc cgtaatgctt gctctggttg
    15961 tatgcgtagt ctggcaagta tttagccgct acgtacttaa ccagccaagt acattaaccg
    16021 atgagctggc acgctttttg atgatctggg taggattatt aggtgctgca tataccgtcg
    16081 gcgcgcagcg acatctctct attgatttat ttgctctcgc cctgaataag cgcaaacaat
    16141 tacttcttag tatagtcatt aacgttctca ttttaggttt tgccggttcg gtcattgtga
    16201 ctggtggcct gaaattaatt gataaaacac tggcaacatc acaggtatct gccgctatgc
    16261 aaataccgat gggatacgta tatatcattc tgccattaag tggagtggta atgatgtttt
    16321 atgccttgtg ttttattaat caaagcattc aacaactgaa acaacctgcg caggaggcca
    16381 gttaatgga
//
LOCUS       DAJTSI010000090        16273 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000090 DAJTSI010000000
VERSION     DAJTSI010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16273
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.090"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..73)
                     /locus_tag="OO327_002620"
     CDS             complement(<1..73)
                     /locus_tag="OO327_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HCR6019113.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQL"
     gene            complement(171..1280)
                     /gene="anmK"
                     /locus_tag="OO327_002621"
     CDS             complement(171..1280)
                     /gene="anmK"
                     /locus_tag="OO327_002621"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR6019114.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQLVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1554..2021
                     /gene="slyB"
                     /locus_tag="OO327_002622"
     CDS             1554..2021
                     /gene="slyB"
                     /locus_tag="OO327_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR6019115.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="OO327_002623"
     CDS             complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="OO327_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR6019116.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2703..2939
                     /locus_tag="OO327_002624"
     CDS             2703..2939
                     /locus_tag="OO327_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR6019117.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2942..3799
                     /locus_tag="OO327_002625"
     CDS             2942..3799
                     /locus_tag="OO327_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6019118.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3799..5811
                     /locus_tag="OO327_002626"
                     /pseudo
     CDS             3799..5811
                     /locus_tag="OO327_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5812..6333)
                     /gene="sodC"
                     /locus_tag="OO327_002627"
     CDS             complement(5812..6333)
                     /gene="sodC"
                     /locus_tag="OO327_002627"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR6019119.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6414..7310)
                     /locus_tag="OO327_002628"
     CDS             complement(6414..7310)
                     /locus_tag="OO327_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR6019120.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7359..7598)
                     /locus_tag="OO327_002629"
     CDS             complement(7359..7598)
                     /locus_tag="OO327_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR6019121.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7701..8300
                     /gene="nemR"
                     /locus_tag="OO327_002630"
     CDS             7701..8300
                     /gene="nemR"
                     /locus_tag="OO327_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR6019122.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8337..9434
                     /gene="nemA"
                     /locus_tag="OO327_002631"
     CDS             8337..9434
                     /gene="nemA"
                     /locus_tag="OO327_002631"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCR6019123.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9515..9922
                     /gene="gloA"
                     /locus_tag="OO327_002632"
     CDS             9515..9922
                     /gene="gloA"
                     /locus_tag="OO327_002632"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR6019124.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10025..10672
                     /gene="rnt"
                     /locus_tag="OO327_002633"
     CDS             10025..10672
                     /gene="rnt"
                     /locus_tag="OO327_002633"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR6019125.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10765..12879
                     /locus_tag="OO327_002634"
     CDS             10765..12879
                     /locus_tag="OO327_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR6019126.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGL
                     SATVRSASDVAAFLGGDRPVTVVNPPTMRHPQIRIVVPVANMDDVSSVASGTGEDSHA
                     GREGSIWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVD
                     AAHFESTSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLEL
                     GIDMGAVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVEC
                     MFAGRLENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRTAPWKDLPRRVFDAT
                     LDMLSGRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPE
                     GEEKAGSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSAQLPF
                     WRGEGNGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATG
                     IVPGSRHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASD
                     DSIVARIPDTDGKLPDAAIFLF"
     gene            12889..14181
                     /locus_tag="OO327_002635"
     CDS             12889..14181
                     /locus_tag="OO327_002635"
                     /inference="COORDINATES: protein motif:HMM:NF020083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019127.1"
                     /translation="MLQIVREAVGSSALFAARFRECAARALLMPGRTPGHRTPLWQQR
                     LRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQISDVTTTTP
                     SPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELLDPQVIRQ
                     VEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLENLLAVKR
                     IFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLRYLRAHAL
                     VTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRSLQAAREA
                     TRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQLAGVGLPA
                     SLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKAG"
     gene            15131..15223
                     /locus_tag="OO327_002636"
     CDS             15131..15223
                     /locus_tag="OO327_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR6019128.1"
                     /translation="MNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(15267..>16273)
                     /locus_tag="OO327_002637"
     CDS             complement(15267..>16273)
                     /locus_tag="OO327_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6019129.1"
                     /translation="QWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPRFKKRGQNAAF
                     RYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWYISIQTESEVS
                     TPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQLSRKVKFSNNW
                     QKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSKSAAGTVSQPG
                     RNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRCACCGHTAKEN
                     RLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA
                     "
BASE COUNT         3711 a         4298 c         4269 g         3995 t
ORIGIN      
        1 ccagttgatt aaaggcgcta caaccggaga gaaggaccgg caaaatgata attaacagtt
       61 ttttcatagt catatcccga agactttcct ggtccggagg gcaaataccc tccttaatct
      121 tccaagtgta acggcagaca cgataagaaa aaattcagtt aattctgata tcacgagttg
      181 gcggggaaaa tagcccccag taccgtctcc tggcttgcgc cagtgacgga aggcagattt
      241 cctggtaatc ccgccagcgt ccgccaggca agccaggcga aagccaatgc ttccatgtca
      301 tcgccactaa tgccaacggc atcggtggtg gtgacttctg tgcctagcag taataccgcc
      361 agacgcgcca tgagtagcgg attacgacta cctccaccac ataccatcaa tcgttcgcag
      421 ccaccgctca acaaaacttg ttcagaaatg gtcacggcgg tgagttctgc cagtgtggcc
      481 tgaacatcac ggggatcaac acccggaaaa tggcgtaaat ggcgctccag ccagccgtag
      541 ttaaagtatt cgcgtccggt gcttttcggt gcaggttgcg agaaatacgg gtcgctgagc
      601 atattttgca gcagtgggag aataacttta cccgcccgtg cccactcggc atctttatcg
      661 taaggtttac ctgcctgacg ccagatccag gcatccatca gcatgttacc aggaccggta
      721 tcgtagccgc caaccagctg cccaggaatg agcagtgaca gattggcgat gccgccaata
      781 ttgagcacca ttcgtcgctc ggttgggtga gccagcaagg catgatggaa cgcaggtacc
      841 agcggtgcgc cttgtcctcc caaggcaata tcgcgacggc gaaaatcacc gacaaccgta
      901 attccggtgc gtgccacaat ttgattgtta tcgccaatct gtaaagtgtg tggtgccacg
      961 cccgtcggtt catgccagac ggtttgaccg tgacagccga tcgcaactat atctctcgcc
     1021 tgcaggtttt gttccttaag caaggcattg accgcatcag caaaaagtcg cccgagttga
     1081 gtatcaagct gtccaaactg cgaaagtgta agctgctggc cctggcaaat atccagtaca
     1141 gcctgtttca gagacaccgg gatcggccag ctcaagcttg ccagctgtgc gacccggtgt
     1201 tcatcaattg tcgccaacac aacatcaaca ccatcaaggc tggtgcctga cataacgcca
     1261 ataaagcggc ccgatttcat agttcatcct ttttcaatct gacgtttgcg caccactcaa
     1321 acataaactt ttcgtgaata ccatgcggaa tgaccgattt ttaccgttgg tagtaaaaca
     1381 ttatcttcaa atcaataatc atcatgaatg ttttgtttat aattggttga tcctactttc
     1441 attatgattt gctcatattt ggtagaacat gtaaccatgg attcacatat gccatatact
     1501 ttgaccatga gggatgcttg cgtggcgttt catggtgaac aggagatttt tcaatgatta
     1561 aacgcgtatt ggttgtttca atggtaggtc tgtctcttgt cggttgtgtt aataacgaca
     1621 ccctgtcagg ggatgtttat accgcttctg aagcgaaaca agtacagaat gtcagctatg
     1681 gcaccatcgt taacgtacgt ccggtacaga ttcagggcgg tgatgattcc aacgttatcg
     1741 gtgcaattgg cggtgctgtt cttggtggtt tcctggggaa tactgttggt ggcggaaccg
     1801 ggcgttctct ggctactgca gcaggcgctg ttgcaggtgg cgtagctggt cagggcgtac
     1861 agagtgcaat gaacaaaacg cagggtgtcg agctggaaat tcgtaaagac gatggtaata
     1921 ccatcatggt ggtacagaaa caaggcaaca ctcgtttctc tccgggccaa cgtgtcgtac
     1981 tggccagcaa tggcagtcag gtgaccgttt ctccgcgcta aataagtttg cgtgtggtca
     2041 ggttactgac cacacgcccc cttcatttca ccctttggcc tgtaactcaa tgatattatg
     2101 ctcaagtttt gcgatgagcg taatcagttg ctccagttcc tctgcggaga tgccatgtaa
     2161 tatttccgcg cgggttttgt taataacagc ttccatttcg ctgatcagtg gctctgcctt
     2221 ttccgtcagt ttaatacgtt tagcccgacg atcgctggca caagtttgac gcgaaattaa
     2281 ccctttttct tccagttggt ccagagtacg gaccagtgat ggctgctcga tgccaatcgc
     2341 ttttgccagt tgaatttgcg actggtctgg aggtaactga tggatattgt gtaacgtaac
     2401 ccaatgggtt tgtgttaact ccagcggttt caggcgatgg tctatcagag cacgccatat
     2461 gcgcaccaac cgtgccagat cagaacctag tggcgattcc aatttcatct ccttataatt
     2521 agcttgctaa gatattatgc ggcttttaga atagtgtgca gcaattgtat tgctaaaaca
     2581 aatgtattgc tgcatttggt taccgtcaga catatttttc agaaattgcg cgtaaatttt
     2641 tcgcacttaa agaatattta ttaatctaac gcaatatatt cggtcgtaaa ggaatctact
     2701 ttgtgaagtt tatgctcaat gcaacaggat tgcccttaca agatctggtg ttcggtgcgt
     2761 ccgtctattt tcctccgttt ttcaaagcat tcgcgtttgg attcgtcatc tggcttgtcg
     2821 tacaccgcct gcttcgtggc tggatctacg ccggcgatat ctggcatccc ttgttaatgg
     2881 atttatcgct gtttgcgatt tgcgtttgcc ttgctctggc aatactgatt gcgtggtaac
     2941 tatgtcaatt aaaacaatta agtatttctc aacaatcatt gtagcgatag ttgcggttct
     3001 tgccggatgg tggctgtgga attattacat gcaatctccg tggacacgcg acgggaaaat
     3061 acgcgcagaa caggtttcta tcaccccaca ggtgtcggga cgtattgttg agctgaatat
     3121 aaaagacaac caactggtta acgcggggga acttctgctc acgatcgata aaacgccctt
     3181 tcagatcgcc gagctgaacg cacaggctca gctggcgaaa gcgcaatctg acctcgcaaa
     3241 agccaataac gaagcgaatc gtcgtcgtca tctctcacaa aattttattt ttgccgaaga
     3301 actcgatacc gctaacctca atgttaaagc gatgcaggcc agtgttaatg ccgcacaggc
     3361 gacgctgaaa cagacacaat ggcaactggc gcaaacggaa attcgcgccc cggtgagtgg
     3421 atgggtgact aacctcacta cccgcatcgg cgattacgct gacaccggga aaccgctgtt
     3481 tgcccttgtc gacagccact cgttttacgt cattggttat tttgaagaaa ccaagttgcg
     3541 ccatatccgc gaaggtgcac cagcacaaat taccctctat agtgataaca aaacgttaca
     3601 gggtcacgtt tccagtatcg gtcgggcgat ttatgatcag agcgttgaaa gtgactcaag
     3661 ccttattccg gatgtgaaac ctaacgtccc ctgggttcgt ctcgcccaac tcgttcccgt
     3721 ccgctttgcg ctggataaag ttccaggcga tgtcacgctg gtgtcgggca ccacctgtag
     3781 cattgccgta ggtcaataat gaacgcatcg tcatggtcct tgcgcaattt gccctggttc
     3841 agggccacgc tggcgcaatg gcgttatgcg ttacgcaata ccattgccat gtgtctggcg
     3901 ctgacggttg cctattattt gaatctggat gaaccctatt gggcgatgac ctcggctgca
     3961 gtggttagct ttcccaccgt tggcggtgtc atcagcaaaa gcctcggacg catggctggc
     4021 agtttactgg gagccattgc ggcactgctt cttgccggac atacgctcaa tgagccgtgg
     4081 ttttttctat tgagcatgtc ggcgtggctt ggcttttgta cctaggcctg tgcgcacttc
     4141 acgaataacg tcgcgtatgc atttcaactg gcgggctaca cggctgccat catcgccttt
     4201 ccgatggtta atattactga ggccagccag ctgtgggata tcgctcaggc gcgcgtttgc
     4261 gaggtgatag tcggtatttt gtgcggcggc atgatgatga tgatcctgcc tagcagttcc
     4321 gatgctactg cccttttaac tgcattgaaa aacatgcacg cccgattact ggaacatgcc
     4381 agtttactct ggcagcctga aacaaacgat gccattcgtg cagcacatga gggggtgatt
     4441 gggcagatac tgaccatgaa tttgctgcgt atccaggctt tctggagcca ctatcgtttt
     4501 cgccagcaaa acgcgcgcct taatgcgcta ctccaccagc aattacgtat gaccagtgtc
     4561 atctccagcc tgcgacgtat gttgctcaac tggccctcac cgccaggtgc cacgcgagaa
     4621 attctcgaac aattgctgac ggcgctcgcc agttcgcaaa cagatgctta caccgtcgca
     4681 cgtattatcg ccccgctacg cccgaccaac gtcgccgact accggcacgt cgccttctgg
     4741 cagcgactac gttatttttg ccgcctttat ctgcaaagta gtcaggaatt acatcgtctg
     4801 caaagcggtg tagatgatca tgccagactc gcacggacat ccgctctggc tcgtcatacc
     4861 gacaacgccg aagctatgtg gagcgggctg cgtacatttt gtacgttgat gatgattggc
     4921 gcatggagta ttgcatcgca atgggatgcc ggtgccaatg cattaacgct ggcagcaatt
     4981 agctgcgtac tctactccgc cgtcgcagca ccgtttaagt cgttgtcact gctgatgcgc
     5041 acgctggtgt tactttcgct attcagcttt gtggtcaaat ttggcctgat ggtccagatt
     5101 agcgatctgt ggcaattttt actgtttctc tttccactgc tggcgacaat gcagcttctt
     5161 aaattgcaga tgcccaaatt tgccgcactg tgggggcaac tgattgtttt tatgggttcg
     5221 tttatcgctg tcactaatcc cccggtgtat gattttgctg attttcttaa ctataatctg
     5281 gcaaaaatcg ttggcgttgc gctggcttgg ctagcgttcg ccattctgcg tccaggatcg
     5341 gatgctcgta aaagccgccg ccatattcgc gcgctgcgcc gggattttgt cgatcagcta
     5401 agccgccatc caacactgag tgaaagcgaa tttgaatcgc tcacttatca tcacgtcagt
     5461 cagttgagta acagccagga tgcgctggct cgccgttggt tattacgctg gggtgtagtg
     5521 ctgctgaact gttctcatgt tgtctggcaa ttgcgcgact gggaatcgcg ttccgatccg
     5581 ttatcgcgag tacgggataa ctgtatttca ctgttgcggg gagtgatgag tgagcgtggc
     5641 gttcagcaaa aatcactggc ggccacactt gaagaattac agcggatttg cgacagcctt
     5701 gcccgtcatc atcaacctgc cgcccgtgag ctggcggcaa ttgtctggcg gctgtactgc
     5761 tcgctttcgc aacttgagca agcaccaccg caaggtacgc tggcctcgta attacttaat
     5821 tacaccacag gcatagcgtt caccgccacc gcccagcggt ttaggttgat cggacatatt
     5881 atcgccgcca acgtggacca tcagcgcctt gtctttgatt tcatccagtg atttcagacg
     5941 aggcgcgatg acggcatcgg tagctttgcc gtcattattg acgaccagtg caggcagatc
     6001 gcctaaatgc ccggtacctt ctggcccttc atgtttaccg gtattttgtg gatcaagatg
     6061 cccgcctgcg gattccgcgg cgctggcttt gccatctttg gtggctggct ggcagcttcc
     6121 tttggcatga atatggaagc catgttcacc ggggggtaat gctttcagat cgggtgaaaa
     6181 ctccagacct ttatcggttt cagtaatggt gacgctacca attgactgcc caaccccttg
     6241 cgacgtgacg aggttcatct cgactttttc actggcagct tgtgcgccgg tcgcaacaac
     6301 cagcgccaga atggccagac taaaacgttt cataggacct ccgttcattg tgcattcctg
     6361 aataagtgta aaccagtgac acatttttga acggcggcta ttcctaaaag tgcttacggt
     6421 acgtcgtacc ccagtgcagc tttacggata cgaaaccatt gttgacgggt cattttcagt
     6481 gtttctgctt cgacagctgc ccgtacgcgc tcaattttac ctgagccgat aattggcagc
     6541 ggctgcgacg gtaaacgtaa tacccaggcg taaaccacct gttcaatcga gcccgcgttt
     6601 aactcctccg ccaccacagc cagttcatca cgcagcggct ggaaataatc atcattaaac
     6661 agacgaccac caccaaggca ggaccacgcc atcggacgaa cacgcagttg ttgtagttgg
     6721 tcgagcgtgc catccagcag taacggctga tgcaccgggg atatttccac ctgattagtg
     6781 gcaagggtaa acggcagacg tgattgcaac agggcaaatt gcgcaggcgt aaagttcgat
     6841 acgccaaaat gacgcacttt gccgctctga tgcagatgtt tgaacgcgtc cgccacttca
     6901 tcggcatcca ttaacgggtc tggtcggtgg attaacagca gatccagatg atcggtcgcg
     6961 agattaatta gcgactgttc ggcgctctta atgatgtgat tgcggtcagt gatgtaatga
     7021 ccaatgacgt tttcttcacg cgcggtcgtc gcgataccgc atttactgac gatttccatc
     7081 cgttcacgca ggtgaggtgc cagtttcagt gcctcgccaa acgccgcttc gcactgatag
     7141 ccaccataaa tatcagcatg gtccacggtg gtcacgccga gatccagatg ctcttcaata
     7201 aaactgacca gctggcgggc ggacatattc cagtccatca atcgccagta gcccatcaca
     7261 aaacgggaaa actccgggcc ttccggcgca atagtaatac gctgaaccat aatcgcttcc
     7321 tcttatcaga tataagagga gtatacgcaa gattaggttc aaaagagtga tggttgctcc
     7381 ggttcgtctg atgacgctgg cttatttgcg cgtaatttgc gcattaatcg ctgccgacaa
     7441 aggcgcagca cctcttgttt ttcgccatcg ctcattttat tccagttaaa acgctcatcc
     7501 cgactacgaa aacagccgcg acaaaacccg cgttcgtcag actggcaaat tccccggcac
     7561 gggctctgga cgggaaagaa ctctaattgc tccgccacct cgccctcctc agataagatt
     7621 attaccatta ttgaagctgt taatgtccaa agtagcaact ttgcttgcac tagaccaact
     7681 ggtctactac actccaacgc atgaacaaac acaccgaaca tgatactcgc gaacatctct
     7741 tggcgacggg cgagcaactt tgcctgcaac gtggattcac cgggatgggg ctaagcgaat
     7801 tactaaaaac cgctgaagtg ccgaaagggt ccttctatca ctactttcgc tctaaagaag
     7861 cgtttggcgt tgccatgctt gagcgtcatt acgccgcata tcaccagcga ctggctgagt
     7921 tgctgcaatc cggcgaaggt aactaccgcg accgcatact ggcttattac cagcaaacac
     7981 tgaaccagtt ttgccaacat ggaaccatca gtggttgcct gacagtaaaa ctctctgccg
     8041 aagtgtgcga tctgtcagaa gatatgcgca gcgcgatgga taaaggtgct cgcggcgtga
     8101 tcgccctgct ctcgcaggct ctggaaaatg gccgtgagaa ccattgttta accttttgtg
     8161 gcgaaccgct acaacaggca caagtgcttt acgcactatg gcttggcgcg aatctgcagg
     8221 ccaaaatttc gcgcagtttc gagccactgg aaaacgcgct ggcccatgta aaaaacatta
     8281 ttgcgacgcc tgccgtttag caggcatttt ttatcaccag acgaccggga gcctttatgt
     8341 catctgaaaa actgtattcc ccactgaaag tgggcgcgat cacggcggca aaccgtattt
     8401 ttatggcacc gctgacgcgt ctgcgcagta ttgaaccggg tgacattcct accccgttga
     8461 tggcggaata ctatcgccaa cgtgccagtg ccggtttgat tattagtgaa gccacgcaaa
     8521 tttctgccca ggcaaaagga tatgcaggtg cgcctggcat ccatagtccg gagcaaattg
     8581 ccgcatggaa aaaaatcacc gctggcgttc atgctgaaaa tggtcatatg gccttgcagc
     8641 tgtggcacac cggacgcatt tctcacgcca gcctgcaacc tggcggtcag gcaccggtag
     8701 cgccttcagc acttagcgcg ggaacacgta cttctctgcg cgatgaaaat ggtcaggcga
     8761 tccgcgttga aacatccatg ccgcgtgcgc ttgaactgga agagattccg ggtatcgtca
     8821 atgatttccg tcaggccatt gctaacgcgc gtgaagccgc ttttgatctg gtagagctcc
     8881 actctgctca cggttatttg ctgcatcagt tcctttcccc ttcttcaaac catcgtaccg
     8941 atcagtacgg cggcagcgtg gaaaatcgcg cacgtttggt actggaagtg gtcgatgccg
     9001 ggattgaaga atggggtgcc gatcgcattg gcattcgcgt ttcgccaatc ggtactttcc
     9061 agaacacaga taacggcccg aatgaagaag ccgatgcact gtatctgatt gaacaactgg
     9121 gtaaacgcgg cattgcttat ctgcatatgt cagaaccaga ttgggcgggg ggtgaaccgt
     9181 atactgatgc gttccgcgaa aaagtacgcg cccgtttcca cggtccgatt atcggcgcag
     9241 gtgcatacac agtagaaaaa gctgaaacgc tgatcggcaa agggttaatt gatgcggtgg
     9301 catttggtcg tgactggatt gcgaacccgg atctggtcgc ccgcttgcag ctcaaagctg
     9361 agcttaaccc acagcgtgcc gaaagtttct acggtggcgg cgcggaaggc tataccgatt
     9421 acccgacgtt gtaatccaac attgcgagcg gcgtaaagcc gccgctatac taaaacaaca
     9481 ttttgaatct gttagccatt ttgaggataa aaagatgcgt cttcttcata ccatgctgcg
     9541 cgttggcgat ttgcaacgct ccatcgattt ttataccaaa gtgctgggca tgaaactgct
     9601 gcgtaccagc gaaaacccgg aatacaaata ctcactggcg tttgttggct acggcccgga
     9661 aaccgaagaa gcggtgattg aactgaccta caactggggc gtggataaat acgaactcgg
     9721 cactgcttat ggtcacatcg cgcttagcgt agataacgcc gctgaagcgt gcgaaaaaat
     9781 ccgtcaaaac gggggtaacg tgacccgtga agcgggtccg gtaaaaggcg gtactacggt
     9841 tatcgcgttt gtggaagatc cggacggtta caaaattgag ttaatcgaag agaaagacgc
     9901 cggtcgcggt ctaggcaact aatctcctgc cgggcgtgaa ctcatcgcgc ccgcatcttt
     9961 actgcatcga caagtaatat ttgtcataat gcgcgctgca atttatccgt attaagagaa
    10021 tcagatgtcc gataacgctc aacttaccgg tctgtgcgac cgttttcgtg gtttttatcc
    10081 tgttgtgatc gatgttgaaa cagccggatt taacgccaaa accgatgcgc tgcttgagat
    10141 tgccgccatc accctgaaaa tggatgaaca aggctggctg atgccggaca ccacattaca
    10201 tttccacgtc gaaccatttg tcggcgcaaa tttgcaacca gaagccctcg ccttcaacgg
    10261 cattgacccg aacgatcccg atcgcggcgc ggtcagcgaa tacgaggcgc tgcacgaaat
    10321 ttttaaagtt gtacgtaaag gtattaaagc gagcggctgt aaccgcgcca ttatggtggc
    10381 gcacaacgcc aattttgatc acagctttat gatggccgcc gcagaacgcg cctcgctgaa
    10441 acgtaacccg ttccaccctt tcgccacttt tgacactgct gcactggccg ggctggcact
    10501 cggacaaacc gtattgtcaa aggcttgcca gaccgctggc atggacttcg acagcaccca
    10561 ggcgcactcc gcgctgtacg acaccgaacg cactgctgtg ctgttttgtg aaatcgtcaa
    10621 ccgctggaaa cgtctgggag gctggccgct acctgccgcc gaagaggtgt aatcgagtcg
    10681 atgcctgatg acatgcaatg attcaggcat ctatagtgag gctattccac gcatcaagca
    10741 tgatattcac ggggaatagc gttaatggca gataatccag acccttcatc gctcctgccg
    10801 gacgtgtttt caccggcgac ccgcgactgg tttcttcgcg cctttaaaca gccgaccgct
    10861 gtccagtcgc aaacctggca tgtggcggcg cgaggcgaac atgcgctggt gattgcaccg
    10921 actggctccg ggaaaacgct ggcagcattt ctctacgccc tcgatcggct cttccgcgaa
    10981 ggcggcgaag atacccgcga ggcgcataag cgtaaaacct cacgcatcct ctatatttca
    11041 ccgataaaag ccctgggcac cgacgttcag cgcaacttgc agatcccgtt gaagggtatt
    11101 gccgatgaac ggcggcggcg cggcgaaacg gtaattattg atgaagtcca cgcggtagcg
    11161 ggcagtaaac gtggtgcgca tctggcgtta agtctggagc ggctcgatgc gctgctccac
    11221 acctcagcac agcgaattgg cctttctgcc actgtgcgct cagccagcga tgtggcagca
    11281 tttcttggtg gcgatcgccc ggttacggta gtcaacccgc ccacaatgcg ccatccgcag
    11341 atacgaattg tcgtaccggt cgccaatatg gatgatgtct catcggtcgc cagcggcacc
    11401 ggcgaagaca gccatgccgg ccgggaaggc tccatctggc catatattga aacgggtatc
    11461 cttgatgaag tgttgcgcca tcgctcgacc attgtcttta ctaattcacg cgggctggcg
    11521 gaaaaactga cggcacgatt aaatgagctt tacgccgcac gcttacagcg ttccccgtct
    11581 atcgccgttg atgcggccca tttcgagtcg acctccggcg caacctctaa ccgtgtacaa
    11641 agtagcgacg tttttattgc ccgctcacac cacggctccg tctctaaaga acaacgagca
    11701 atcaccgaac aggcgctgaa atcgggtgaa ttacgctgcg tggtcgcaac ctccagtctt
    11761 gaactgggga ttgatatggg cgcggtggat ctggtgattc aggtggcaac gccgctttct
    11821 gttgccagtg ggttacaacg cattggtcgc gccggacatc aggttggcgg tgtatctaaa
    11881 gggctgtttt tcccccgtac ccggcgtgat ttagtcgatt ccgcagtcat tgtagagtgt
    11941 atgttcgcag gcaggctgga aaacctgaca ccaccgcata atcctctcga cgtccttgcg
    12001 cagcaaaccg ttgccgccgc ggcgatggat gcattacagg tagacgaatg gtactcccgc
    12061 gtacgccgta ccgcaccgtg gaaagatctg ccaagacgtg tttttgacgc cacgctggat
    12121 atgctttccg ggcgctatcc ctctggcgat ttttctgctt ttcgccccaa actggtctgg
    12181 aacagggaga ccgggatatt gaccgcccga cctggcgctc aattgttggc ggttaccagc
    12241 ggcggcacca ttccggatcg tggcatgtat agcgtgttat tacccgaagg tgaagaaaag
    12301 gccggttcgc ggcgggtggg tgaactggat gaggagatgg tatatgagtc gcgggtgaac
    12361 gacattatca ctctcggcgc tacctcatgg cggatccagc aaatcacccg cgatcaggtg
    12421 attgtgactc ctgctccggg tcgttctgcc cagctcccct tctggcgtgg tgaaggtaac
    12481 ggacgtccgg ctgaattagg cgagatgatc ggcgattttc ttcatttact ggcggatggc
    12541 gcgttctttt ccgggactat tcccccgtgg ctggcagaag aaaatacgaa cgccaatatt
    12601 caggggttga ttgacgagca acgcaacgcg acgggcatcg ttccggggag tcgccatctg
    12661 gtcctcgaac ggtgccgtga tgaaattggc gactggcgta ttattttgca ctctccctat
    12721 ggaagacggg tgcatgaacc ctgggcgctg gcgattgccg ggcgaataca tgccctatgg
    12781 ggcgctgacg cgtctgtggt cgccagtgat gacagcattg ttgcgcgcat tcctgacact
    12841 gatggtaaat tgcccgacgc cgcgattttt ttgttttaac cagaaaagtt gctgcaaatt
    12901 gtccgcgagg cggtaggcag ctcggcactt ttcgccgccc gttttcgcga atgcgccgcg
    12961 cgggcattat taatgccggg ccgcacgccg ggccatcgca ccccgctttg gcaacaacgg
    13021 ctgcgcgcca gtcagttgct ggaaatcgct cagggatatc cggattttcc ggtcattctc
    13081 gaaaccctac gcgaatgtct gcaagatgtt tatgatcttc ccgcactgga acgtttgatg
    13141 cgtcgcctga acggtggcga aattcaaata tccgatgtaa cgaccactac gccctcgcct
    13201 ttcgccacaa gtttattgtt cggctatgtc gcggaattta tgtaccagag cgacgccccg
    13261 ctggcagagc gccgggcatc cgtactgtcg ctggacagcg agttactgcg caatctactc
    13321 ggacaggtcg atccggggga attactcgac ccgcaggtca ttcgccaggt ggaagaagag
    13381 ttgcaacgac tggctcctgg tagaagagcg aaaggtgaag aaggattgtt cgacctgctg
    13441 cgcgaactgg ggccaatgac cgttgaagac ctggcgcaac ggcatacagg cagcagtgaa
    13501 gaggttgcgt cgtatctgga aaatcttctt gcagtaaaac gaatcttccc agcgatgatt
    13561 agcggacagg agcgtcttgc ctgtatggat gatgccgcca ggctgcgtga tgccctcggc
    13621 gtacggctac cagagtcatt gccagagatt tatttacata gagtcagtta cccgcttcgc
    13681 gacctctttc tgcgctatct ccgggctcat gctctggtca cggctgaaca actggctcat
    13741 gagtttagtc tcggtattgc cattgtcgaa gagcagcttc agcaactgcg tgaacagggt
    13801 ctggtgatga atctgcaaca agacatctgg gtgagcgatg aagtatttcg tcgtctgcgt
    13861 ttgcgctcgc tgcaagccgc cagagaagcg acgcgtcccg ttgcagccac gacctatgcg
    13921 cgattgctgc tggaacgtca gggcgtatta cccgccaccg atggtagccc ggcgctcttt
    13981 gcctcaacat ctccaggcgt ttatgagggc gtagatggag tgatgcgggt gatcgaacag
    14041 cttgccggag tcggtttacc cgcctcactc tgggaaagcc agatcctgcc tgcccgcgta
    14101 cgcgactatt cgccagaaat gctcgatgaa ttactggcaa ccggagcggt tatctggtcg
    14161 ggacaaaaaa aagctgggtg aagatgacgg cctggtggca ctgcatctac agaaatatgc
    14221 cgcagaatcg ttcactcccg ccgaagcgga tcaggcgaat cgttcggcgc tgcagcaagc
    14281 gataatcgct gttctggctg acggaggagc ctggtttgca caacaaatca gccaacggat
    14341 acgcgataaa atcgtcgaat cggttgattc ctcggcactg caagaggcgt tatgggcgct
    14401 ggtctggcaa ggcgtcatta ccagcgatat ttgggcaccg ttacgcgccc tcacccgcag
    14461 cagttccaac gcacgcacct caactcgccg cagtcaccgg gctcgtcgtg ggcgtcctgt
    14521 ctatgcacaa cccgtctcgc cgcgggtatc ttacaacaca ccaaatctgg ctggacgctg
    14581 gtcgttattg caggtggagc cactaaacga taccgaaagg atgctggcgc tggcggaaaa
    14641 tatgctcgac cgctacggca tcatcagtcg tcaggcggtg atagccgaaa atatccctgg
    14701 cgggtttcca tcgatgcaaa cactttgtcg aagtatggaa gactccgggc gaattatgcg
    14761 aggtcgtttt gtagaaggtc tgggtggcgc gcaattcgct gaacgtctga ctattgaccg
    14821 attgcgcgat ctggcgacac aagccacgca aacacgccac tatacaccag tggcgctctc
    14881 cgccaacgat ccggctaatg tgtggggaaa tcttctgccc tgggctgcac atccggcaac
    14941 gctggtacca acgcgtcggg cgggtgcgct ggtggtcgtt tctggcggca aattgttact
    15001 ctatctggcg caaggcggca aaaaaatgct ggtctggcag gaaaaggagg aattactcgc
    15061 cccagagatt ttccaggcgc tgaccaccgc actgcgtcgc gaaccaaggc tgcgctttac
    15121 gctaacagaa gtgaatgatc ttccggtccg gcaaacgccg atgtttacgc tgctgcgtga
    15181 ggcgggattt tcaagttcgc cacaagggct ggattgggga tagttgacat cctccacgcc
    15241 ctgaaagacg tggttccctg ctacgttcaa gctgtcgcct gaatcatttc ggtgggttcc
    15301 tgcttcaacg agcggcctga ctgcaccatc tctccacagg caagaacggc gtgccccgcc
    15361 gctaaaatgt tacgagcgcc atttacatcg gcgttcgctg tatatccaca tacctggcat
    15421 ctgaatttac tttgtgacag gcggttttct ttcgctgtat gaccacagca cgcgcaacgc
    15481 tggcttgtgt acgctggcgg aacagcaagc acctgaccgc cactccagag ctgcttgtac
    15541 tcaagctggc ggcgcatttc ataccagccc tgatccagta tcgaacggtt taaacctgat
    15601 tttgcccgga cattgcgccc cggctggctg accgtacccg ctgctgactt tgacatgttg
    15661 ctgaccttca aatcctcaat gactatcatt gcgtggtttt tgctgacggt cgttgtgact
    15721 ttgtgaaggt agtccctgcg gatatttgcg atacaggaat gcagtcgctg tattttgcgt
    15781 ttctgcttct gccagttgtt gctgaatttg accttccggc ttaactgtcg ctgaagtctc
    15841 gccagcgtct tctggttttt ttgaaaactg tttacaggct caaagactgt gccatctgac
    15901 agcgtggcga gtttagccac gccagcatcc agcccgatca ttgatgctga agggtgaacc
    15961 ggagttgata cttcactttc tgtctgaata ctgatgtacc acgtaccgca ggactggctg
    16021 acagtgacat ttttcacaac tcccgtgacc tgacggctat tccggtagcg catccagcca
    16081 agtttcggca gaaaaatacg gctgttttcc tgatcgagct taacgtcctg cgggtagcgg
    16141 aatgcagcat tctgtccccg ctttttgaat cggggaaaag cagcccgctt ctggaagaag
    16201 tttttgtaag cccgctcaag gtctttcagt gactgttgca atggctgtga gggtgaatct
    16261 ttaagccatt gcg
//
LOCUS       DAJTSI010000091        16026 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000091 DAJTSI010000000
VERSION     DAJTSI010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16026
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.091"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(77..1178)
                     /gene="gldA"
                     /locus_tag="OO327_002638"
                     /pseudo
     CDS             complement(77..1178)
                     /gene="gldA"
                     /locus_tag="OO327_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            complement(1189..1851)
                     /gene="fsa"
                     /locus_tag="OO327_002639"
                     /pseudo
     CDS             complement(1189..1851)
                     /gene="fsa"
                     /locus_tag="OO327_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(1863..4364)
                     /gene="ptsP"
                     /locus_tag="OO327_002640"
                     /pseudo
     CDS             complement(1863..4364)
                     /gene="ptsP"
                     /locus_tag="OO327_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            4673..5752
                     /locus_tag="OO327_002641"
     CDS             4673..5752
                     /locus_tag="OO327_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR6019130.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            5767..6087
                     /locus_tag="OO327_002642"
     CDS             5767..6087
                     /locus_tag="OO327_002642"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR6019131.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            6138..8435
                     /locus_tag="OO327_002643"
     CDS             6138..8435
                     /locus_tag="OO327_002643"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR6019132.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            8401..9279
                     /locus_tag="OO327_002644"
     CDS             8401..9279
                     /locus_tag="OO327_002644"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR6019133.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            9281..9622
                     /locus_tag="OO327_002645"
     CDS             9281..9622
                     /locus_tag="OO327_002645"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR6019134.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(9609..10460)
                     /locus_tag="OO327_002646"
     CDS             complement(9609..10460)
                     /locus_tag="OO327_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6019135.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10609..12342)
                     /locus_tag="OO327_002647"
     CDS             complement(10609..12342)
                     /locus_tag="OO327_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR6019136.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(12525..15176)
                     /gene="ppc"
                     /locus_tag="OO327_002648"
     CDS             complement(12525..15176)
                     /gene="ppc"
                     /locus_tag="OO327_002648"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR6019137.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(15528..>16026)
                     /locus_tag="OO327_002649"
     CDS             complement(15528..>16026)
                     /locus_tag="OO327_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HCR6019138.1"
                     /translation="LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMD
                     IRPQPGMTLNELNGLLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKL
                     LGAKTEVVNYCTEAPFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIH
                     HFCWH"
BASE COUNT         3607 a         4108 c         4423 g         3888 t
ORIGIN      
        1 gcgtaggcct gataagcgta gcgcatcagg caattttgcg ttcaaactcc cggacaagcc
       61 gggagtttgg agtaggttat tcccactctt gcaggaaacg ctgaccgtac tggtcagcta
      121 ccagcagtgc ggcgtaaacc tgatctggcg tcgcgccgcc aggcatgttg tggatggttt
      181 caccttctgc acaagatgct tttgcaacaa tgcgcatttt cgccgggaca tcttctttaa
      241 tatccagttg agcgagagtt attggcaaac ctaccgcatg gctaagtgca gctacggttt
      301 cgatttcctc caccggcgcg ttttccagta ccagctgcgt cagcgtacca aatgccactt
      361 ttcaccgtga taatagtgat gcgcgtccgg gatagcggtc aggccgttat gcaccgcgtg
      421 agccgccgcc agccgccgct ttcaaaacca acgccgctca aataggtgtt cgcttcaatc
      481 acgcgctcca gcgccggggt tactacatgc tgttcggcgg caagcatcgc tttttcgcct
      541 tcttccagca gggtgttgta gcacagttca gccagtgcca gcgccgcctg ggtgcacttg
      601 ccgcccgcca tggtggtcgc gccgctacga gagcaagcac gcgcttcaaa ccaggttgcc
      661 agcgcatcgc cgatacccgc tgctaacaga cgtgcaggtg cgcccgcgac gattttggtg
      721 tcgacaatga ccatattcgg gttatttggc aacagcagat agcggtcaaa ctcaccctca
      781 tcggtgtaga taacagacaa tgcgctgcac ggtgcatcgg tcgaggcgat agtcggtgcg
      841 atcgctaccg gaacacccat gaaatgtgcc agtgctttgg cagtatcgag ggtttttccg
      901 ccaccgatac cgagaattgc gccacactgc gcagtctccg cgatgccacg cagacggtcg
      961 atctcatttt gcgaacattc accgccaaac ggcgtaattt ctactaccag tccagcatct
     1021 ttaaagcttt tctcgacagt ggattgagca aaaactaaaa caaatttgtc acccaccact
     1081 aaccagcgtt ctgccagcgg cttcaggtat tcgcccagac gattaatcac atcagcgccc
     1141 tggatgtatt tacccggtga ttgaataatg cggtccataa ttgctccttt agagatgagt
     1201 agtgccaaat gcggcattcc agtcgtgttc gaacttctct atagctgact ctaccgcagg
     1261 ggtgttgagc atttgttgcg ctacatctaa gggcagggtg atggattcac atcctgccag
     1321 taaacagtcc agcgcctgac gcggcgtttt aaagctggct gccagcacca tgctttctgg
     1381 cgcgtgcatt tctaacagcg tttgcagctc ctgaaccgta cgaatgccgt ccccgccctg
     1441 ggcatctacg cggttaacat acggagcaac gtattttgcc cctgccagtg cggctaataa
     1501 cccttctgcg gcgctatata cagcggtgcc aagtgtagta ataccctatt ttttcagtat
     1561 tttaattgct gccagacctt cggaagtcac cgggattttc accacaatac ccggaatagc
     1621 gtcgcgcagg cgcttcgctt cttccaccat cccctgcgcg tcgcggctca tggtctgagc
     1681 aaacagaatg ccctcatcac caatcgcttt ttgcagacgc ggcagcactt cccagatgga
     1741 ctccttgctg gcagcgataa tgctcgggtt agttgtcacc ccggcaatcg ggaatatgcg
     1801 tgccagacgt tcgacttctg cgacgttagc ggtgtccaga tacagttcca tgatgtttcc
     1861 ttttacagtt ccagttcatg ttgcagcagg ctggcgatag cgtcggcaga ggcggcgtta
     1921 accagcgcgt tacggaattc ttcgtgcatg atgcggcgag cgaggcgcga gaaaatgcgc
     1981 atgtgctgat cgcccgcagc gtgtttgttc agggttaaca taatgatgaa ttgcgcttca
     2041 tcatcgcccc agcgcaccgg cgcttgcaga cgcgccacgc tgatggtgga ttgctcaatg
     2101 tgttctgatt tgctgtgtgg aatggcaaaa ctaaagccca gaccggtaga gaaaacggcc
     2161 tcgcgcgccc ataagtcggc ttccagttta cgcggatagc ggcagcggcc cgccagcagc
     2221 aggttatcgg tcatgccttt gagcacttct tctttgctgc gccagtcact ttccagcgtg
     2281 atgcactcgg cggtgaccag cggtgcgtcc tgttgggtca tgcggaattg cgccagcagg
     2341 tgttccactt ccagcgaagt acggcaggcc attgcctggt tgagcaactt gcggcactca
     2401 cggctatcaa gttgcgccat ccgcgctttc gccgccggaa ttgatggtgc gctcatgctg
     2461 agttcatcca gccctaagcc gaccagcaac ggcagcacgg aacctttcgc tcccagctca
     2521 ccgcacagac caatccattt accctggcga tgcaccgcct gcacgtcgta atcgagcgcc
     2581 cgcaagaatg ccggattcag gctgttgtag tgacgagtaa ccttagcgtt atcgcgatcc
     2641 actgccagca gatattgcgt caggtcatta ctaccaatac taaagaaatc aatctcttcg
     2701 cagcattgat cgatgatgaa catcaccgac ggcacttcca gcataatgcc gagctggatc
     2761 ttctcatcaa acggaatgtg ttcgttacgt agttgctgtt tggcttctgc cagtttttct
     2821 ttcacccata agatctcttc cattgaggag atcatcggga tcatgatttt caggctgccg
     2881 tgagcggagg cgcgcaggat cgagcgtagt tgtgtggtaa ataacgacgc gtattcttca
     2941 taaatacgca cggcgcgata accgaggaac gggtttgcct ctgcgggaat gtttagataa
     3001 tcaacgggtt tgtcgccgcc aatgtccata gtgcgcacaa taatgctgcg tccgttggca
     3061 gactccagcg cctgacaaaa aatgttgtac agctcgcttt cgcccggtgc actggtgcga
     3121 tccatataga gcatttcagt gcggaacaaa ccaacgcctt ccgcaccatt gccgaatgcg
     3181 gcctgcgctt ccacggagtg agcgatgtta gcggcaattt caatgcggat accgtcagcg
     3241 gtacgggctt gttgggtcag ctagacacgc tgttgctcac gcagggcgtc ctgtacgcgg
     3301 gcttcttgct gataataacg agctacggct tctcctggct caaccacaat cgccccggcg
     3361 ttgccgtcga tataaatcgt ttgatgctgc cacggagtga gggcatcaat atccacacca
     3421 accagcgtcg gaatgttgaa cgaacgggca aggatcaccg tatgtgaggt ggtgccgccg
     3481 cttttcagta acaatccttt gaggtgattt ttatccagtt cgaggaactg gctgggggtc
     3541 agttcatcag ccatacaaat ggcggactgc gtcagtttgc ccggtgccgg gaagcgttgc
     3601 tcaccgtaga tttgctggag taactggaag cagacgtcgc gtacgtccag ggcacgttct
     3661 tgcaggtatc tgctgctgga acgggcaaac tcttcgcaaa agtgattcgc actggcgaca
     3721 atcgcttcgg cgcagcttaa tccggcgctg acgcctgcca gtaaatgttc gtgcagggaa
     3781 gtatcgccag ccagggatcg gtgcgcttcc agaatcgcgc tggtagcacc gtcgctatcc
     3841 agcagacgaa actcaatgtt tttcagtacc agcgtcaggc cgttttccag tgcggattgc
     3901 tcggcgtcaa cgtctttggc tgcgggaaga ttactcagcg cattgagatc taaagaagag
     3961 atcggcgtca gaatgccgcc tgcactaccg ctgcacacgg tgcgggcgcg gataatttgc
     4021 ggattcagat tggtcagtga aaccggcagt ggttccagtt cgtcagattt aacttccgcc
     4081 agcggcgcat cgcagtgggg gaattcatcg cgcagccatt ggcttaaacg ctggtgccct
     4141 tcctgttcgt cggccccgga aatcagtagc tggcagttat cacccaccag cgtatcggtg
     4201 ccaatcagcg ccagagcgct tttggcgttg cctttacggt cagtacgcag gttatgccac
     4261 tcaatttgag atgaaaaagt attacacagc gtttcaacgt ggcttgccgg acgcgcatgt
     4321 acgccgttag gtagctcaca aataaattcc acaatcaggg ccattgcctt tctcccggta
     4381 cggttttctg tttacaggat aagagcgcac ggtaacggcc tgccatgtga caaatctgcc
     4441 aaaagctgga caaatgtaat gtaaccgtca atttgcgacg cgtctcacaa gacgccgttt
     4501 tgcggcatgc ttccggatta tcgcaagtta tgaggcggat cgcatttttg tactgatatt
     4561 acaaaagtcc agtaaatggc ctttttatcc actgtttgct ccgcctgcga ttgcctattg
     4621 tttcctccat caaaaatatg gacatgggct tttgcaagcc cgggagcagc ttatgaatga
     4681 gttggtgcag atcctgaaaa atacccgtca gcatttaatg acgggcgttt cacacatgat
     4741 tcccttcgtg gtatcgggcg gtattttgct ggcggtttcc gtcatgttgt atggcaaagg
     4801 cgcagtgccg gatgccgtag ccgatccgaa tttgaaaaaa ctgtttgata tcggcgttgc
     4861 aggtttgacg ctgatggtgc ctttcctcgc cgcttacatt ggttactcca ttgcagagcg
     4921 ttctgcgctg gctccgtgcg ctatcggtgc ctgggttggt aacagctttg gtgcgggctt
     4981 ctttggtgcg ctgatcgccg ggattatcgg cggcatcgtg gtgcattacc tgaagaaaat
     5041 tccggtgcat aaagttctgc gctcggtgat gcccatcttc attattccta tcatcggcac
     5101 actgattacc gcaggcatca tgatgtgggg cttgggcgag cctgtagggg cgttgaccaa
     5161 cagcctgact cagtggcttc aggggatgca gcagggcagc attgttatgc tggcggtgat
     5221 catggggctg atgctggcgt tcgatatggg cggtccggtt aacaaagtgg cctatgcctt
     5281 catgctgatt tgcgttgctc agggtgttta taccgtggtg gccattgctg ccgtgggtat
     5341 ttgtatcccg ccgctgggga tggggctggc gacgctgatt ggtcgtaaaa atttctccgc
     5401 agaagagcgc gaaaccggca aagcggcact ggtgatgggc tgtgttggcg ttactgaagg
     5461 ggcgattcct ttcgccgctg ccgatccgct gcgtgtcatt ccttccatta tggtcggttc
     5521 agtttgtggt gcagtaactg cggcgctggt cggtgcgcag tgctatgcag gctggggtgg
     5581 tctgattgtg ctgccggtgg ttgaaggtaa gttgggttat atcgcagcag tggctgtcgg
     5641 agcagtggtg acggctgttt gtgtgaacgt gctgaaaagt ctggcgcgta aaaatgggtc
     5701 ttcgactgat gaaaaagaag acgacctgga tttggatttt gaaattaatt aattgaggaa
     5761 cgagccatga cgaaaattat tgcagtaacc gcatgtcctt caggtgttgc ccatacctat
     5821 atggcagcag aggcgctgga aagtgccgcg aaagcgaaag gctgggaagt gaaagtagaa
     5881 acccaggggt caattggtct ggaaaatgaa ctgactgcgg aagacgtggc gagcgctgat
     5941 atggtgattc tgaccaaaga tatcggcatc aagtttgaag agcgttttgc tggcaagacc
     6001 atcgtgcgcg tcaacatcag cgatgcagta aaacgtgctg acgccattat gagcaaaatt
     6061 gaagctcacc tggcgcaaac cgcttaagtt cttctcccgc tcgcaagggc gggttcgctt
     6121 tcccacagga gttcctcatg acgaatcgta tctctcgcct caaaactgca ctgtttgcca
     6181 atacccgtga aatctcgctg gagcgggcgc tgctttatac cgccagccat cggcaaaccg
     6241 aaggcgaacc ggtgatattg cgccgggcga aagcaacagc gtatatcctt gaacatgttg
     6301 aaatttcgat tcgtgatgaa gaactgattg ccggtaaccg caccgtaaaa ccgcgcgccg
     6361 ggattatgtc gccggaaatg gacccttact ggctgctgaa agagctggat caattcccga
     6421 cgcgtccgca ggaccgcttt gctatcagcg aagaagataa acgtatctac cgtgaagagt
     6481 tgttcccgta ctgggaaaaa cgttcgatga aagatttcat caacgggcag atgacagatg
     6541 aagtaaaagc cgcgaccaac acgcagattt tcagcatcaa ccagacggat aaaggccagg
     6601 ggcacattat tattgattac ccacgcctgt tgaatcacgg tctgggtgag ctggtggcac
     6661 agatgcagca acattgtcag caacagccgg agaatcactt ttatcaggcc gcgttgttac
     6721 tgctggaagc ctcgcagaaa cacattttgc gttacgccga actggcggaa acgatggcgg
     6781 caaactgcac agatgcccag cgtcgcgaag agctgctgac tattgcggag atctcccgcc
     6841 ataacgcgca acataagccg cagacgttct ggcaggcgtg ccagttattc tggtacatga
     6901 acatcattct gcaatacgaa tccaacgcca gttcgctgtc tttagggcgc ttcgaccagt
     6961 atatgttgcc gttctatcag gcatctttaa cccagggcga agatccggcg ttcctgaaag
     7021 aactgctcga atctttatgg gtgaaatgca acgacatcgt gctgttgcgc tccaccagta
     7081 gcgcgcgtta tttcgccggt ttcccgaccg gctataccgc actgctcggc gggttaaccg
     7141 agaacggacg tagcgcggtg aacgtgcttt cgttcctttg ccttgacgcc tatcaaagcg
     7201 tgcaattacc gcaaccgaac ctcggcgtgc gcactaacgc cttgatcgac acgccgttcc
     7261 tgatgaaaac cgccgaaacc attcgcctcg gcaccggtat tccgcaaatc tttaacgatg
     7321 aagtggtggt gccagcgttc ctcaaccgtg gcgtttcgct ggaagatgcg cgcgactatt
     7381 ccgtagtggg ctgtgtggaa ttatctattc ccggcagaac ctacggcttg catgacatcg
     7441 cgatgtttaa tctgctgaaa gtgatggaaa tctgcctgca tgaaaatgaa ggcaatgctg
     7501 cgctgactta tgaaggttta ctggagcaga tccgcgccaa gatcagccac tacatcaccc
     7561 tgatggttga gggcagcaat atttgtgata tcggccatcg cgactgggca cctgtaccgc
     7621 tgctctcgtc ttttatcagc gattgtctgg aaaaaggtcg cgatattacc gatggcggcg
     7681 cgcgttataa cttctccggc gtacagggaa ttggtatcgc caacctgagc gattctctcc
     7741 atgcgttgaa agggatggtt tttgagcaac agcgtttaag ttttgacgaa ttgctgtcgg
     7801 tattaaaagc caactttgca acgccagaag gcgaaaaagt ccgcgctcgc ttaattaacc
     7861 gctttgagaa atacggtaac gatatcgacg aggtggataa cattagcgcc gaactgttgc
     7921 gccactactg caaagaagtg gaaaaatacc agaacccgcg cggcggttac ttcacgccgg
     7981 gatcgtatac cgtttctgct cacgttccgt tgggatcggt ggttggcgcg acgccagacg
     8041 gtcgttttgc cggggaacaa ctggcagacg gcggcttgtc acctatgctg ggtcaggacg
     8101 cacaagggcc aacggcggta ctgaagtcag tcagtaagct cgataatacg ctgctgtcta
     8161 acggtacgtt gctgaacgtg aaattcactc cggcgaccct ggaaggtgaa gcgggattac
     8221 gcaaactggc cgacttctta cgggtgttta cccagcttaa gttgcagcat attcagttta
     8281 acgtggtgaa cgccgacacg ttgcgggaag cgcaacagcg cccacaagat tatgccgggc
     8341 tggtggtgcg cgttgccgga tacagcgcct tctttgtcga actgtcgaag gagatccagg
     8401 atgacatcat ccgccggaca gcgcatcagc tgtaacgttg tggaaacgcg ccgcgatgat
     8461 gtggcgcgca ttttcaacat tcagcgttat tcactgaatg acggcgaggg cattcgtacg
     8521 gtggtctttt ttaaaggctg tccgcatctt tgcccgtggt gtgctaatcc ggagtcgatc
     8581 tccggcaaaa tccagacggt acgcagagag gcgaaatgtc tgcactgtgc gaaatgtttg
     8641 cgtgatgcgg atgaatgccc ctccggggcg tttgaacgga ttggtcgcga tatcagcctt
     8701 gacgctctgg aacgggaagt gatgaaagat gacattttct ttcgcacgtc cggcggcggc
     8761 gtcacgcttt ctggcggcga agtgttaatg caggcggagt ttgctacccg ttttttacag
     8821 cgactgcggc tgtggggtgt ctcatgcgcc attgaaactg ccggagacgc gccagcaagc
     8881 aagctgttac cgctggcgaa attgtgcgat gaagtgctgt tcgatttaaa aattatggac
     8941 gcgactcagg cgcgggatgt ggtgaagatg aacctgccac gcgtgctgga gaatctgcgt
     9001 ttgctggtga gtgagggtgt caacgtgatc ccgcgtttac cgttgatccc tggtttcacg
     9061 ctcagccggg agaatatgca gcaggcgctg gatgtactga tcccgctgaa aatcaggcag
     9121 atccatctgt taccgtttca tcagtacggc gaaccgaaat accgcctgct ggggaaaaca
     9181 tggtcgatga aagaggtgcc tgcgccgtcg tcagccgatg tgacaacgat gcgcgaaatg
     9241 gcagaacggg ccggatttca ggttaccgtg ggaggttaaa atggcatacc tggtggcagt
     9301 aaccgcctgc gtaagcggcg tggcgcatac ttatatggcg gcggaacggc tggaaaagtt
     9361 gtgccagtta gagaagtggg gcgtcagcat tgaaactcag ggcgcgctgg gaacggagaa
     9421 tcgtttagcg gacgaggata tccgtcgggc ggatgttgct ctgttgatta cggatatcga
     9481 gcttgccggt gccgagcgat ttgaacattg ccgctatgtg caatgcagca tctacgcatt
     9541 cctgcgtgag ccgcagcggg taatgagcgc ggtgcgcaaa gtgctttctg cgccgcagca
     9601 aacccatctt attctggagt agtcggtttt tctgtcagtt ggctgtggta ctgccggcga
     9661 tattccgacg gcgagtgttc ggtgttttta cgaaacagac ggcagaagta gttgctgtcg
     9721 acaaagccgc aggcgtgcgc cacttctttt accttcaggt catagccttt caataacgtc
     9781 ttagcgtgct ccagtcgcgt gtgattcagg tattcgttaa aaccaatggc ccccgttttt
     9841 tgaaacaggt gcgagagata gtttggtgaa atatagaatg cctgtgcgac agattcgcgg
     9901 gtaagcgcgg aggcatagcg ttcgtcgata taatcgcgaa tagcttcaaa tagtgcctgg
     9961 ctgcgtgagg cggtctggat ttggctgccg agcaaatcgc ggcagtggct aagcaggctg
    10021 gctacgataa gccttgccgt ttgctgctcc tgcggctgca tttgcatttc attgagcgtt
    10081 tgtagcagaa aagaacctat gcgtgggccg cgtcgggcga cgtgttgctt cgccagattt
    10141 tgatattgtt tgccatccca ttgcacgacg ctgaagccga gttgctgttt gccaaacagc
    10201 acgctaaagg tggtagcggg tgttttccat tgtgggaaat tccagccacc agcggcaacg
    10261 tacagcacat cgccgggaac taacgttgct ccagcgccgg tatcaacaaa ctcaccttcc
    10321 agcacaattt ccagccgtgg aaaatcgacc tgatacgcaa gatcgggcac cgggccgtta
    10381 ctgctggcaa agtaaatctg gcgcagggac agcgggccgt tgatcaggcg ggagagcaga
    10441 tagctgacgt cgtgatacat gttattccct taggtatgta gacgggaaaa cagtaactgg
    10501 cattattaaa aaaggccacc tgaaaagtgt ggcctgaggg gagttcgatg taaacattgc
    10561 ggcagtatcg catccggcaa atagcgccag acgcgataca aaggtcatct actgattacc
    10621 cacctgatcg ccatacggca gagtgtcgta atcgatcagc gcgtttttct tgtacgggtt
    10681 accaatccag cgggtagttt ctttgaactg cggattcacc actgaacggg ttggatcgta
    10741 accgtcgtaa gataagcccg ccaaatctga ccaggtgtgg atcagttccg ccaggctgta
    10801 tttacgatcg acatcctgtg agaaatcacg gggatgagtc gcttgccatt tttccgacgt
    10861 ccacagcagg aacggaatgg tgtacatgtg gcgcgtcggg ttgtcttcgt tacgcccctg
    10921 agttttgtgc ggcggcgtgt cgtaaacctc ttcaccgtgg tcagagaagt aaaccaggaa
    10981 tccgttcggg tctgccgctt taaagtcttt aatcaggctg gcaaccacat gatcgttata
    11041 caggttagcg ttgtcataat cgttatatga ttccagctct tctgcgctta atcctggcgg
    11101 aacatgatcg gtattgccat caaacttgcc ctgattttcc gggtagcggt atttgtattt
    11161 gatatgcgta cccagcagat gaacgatgat cagtttcttc ggcgcagggt cattcagcac
    11221 atcctggaac ggcttcagca cgttggtgtc gtattcacgc gcactctgcg tacgttgctg
    11281 gttcatgtag tactgcttgt cggtctggcg cgaaaatacc gtcagcatgg tattgcgggc
    11341 ggtcatcgtc tgctggttgg tgatccagaa ggttttataa cccgcctgtt tcatcatgtt
    11401 catcagcgac ggctgcgtca gatacagatc cgggttcttt tcattggcaa aggtcagcgc
    11461 ctgttgcagg atttcaatgg tgtacggacg agacgtaact acgttattaa acacggtcag
    11521 attcggatcg gttttatgca gcgcatccag ctccggcgtg gtttcacgcg gataaccgta
    11581 cagactcatg cgtccgcgct gggtcgactc gccaatcacc agcactaaag tgcgcggttc
    11641 gttacccgat tcatctttga aattagccag tggcggcaag gcattatttt cattcagtaa
    11701 ctttgttagc gagtttagtt gctgacgata ctgataatag ccggtcagga attgccacgg
    11761 tgcggcaggc tccatacgcg aggctaggtt atccaacgtt ttctcaaacg gcttgttttt
    11821 gataaacgta ttcatggcga tcggatgcag aatcaagcca taaagcaggg caaaagagac
    11881 aacataacgc cacggctttg gaatatagac cgggcgcagg cgtgtccaca gcagaactgc
    11941 caccgccgta taggccagcg cgataagcac aattttcagg ctgaaatact ggcttaaata
    12001 ctcgctggct tcgttggtgt tggtttcgaa catcacaaac agaacgctct gcgagaactc
    12061 ctgcccgtag atgacgtagt agcacagcgc cgccagagag gccgtccata gcaccacgcc
    12121 gatcactgcg gcaataattt taatccgctt cggaaagagg aataccggga tcaaccacag
    12181 cgagctgaat aacagcgagt cgcgaatgcc gttggtgcca ctataaccac tgatgtaaat
    12241 aatggcctgt agcagagtag agaaaaacca aaagtagagc agtgcccaac ccagggcttt
    12301 ccagctagaa aggggtttag cctggacttc tgtggaatgc atagtaagaa cctgtcttga
    12361 aaaaaatatc gccgaatata acgacaattc cttaaggata tctgaaggta tattcagaat
    12421 ttgaataaaa tgcagacaga aatatattga aaacgagggt gttagaacag aagtatttca
    12481 gaaaaccctc gcgcaaaagc acgagggttt gcagaagagg aagattagcc ggtattacgc
    12541 atacctgccg caatcccggc aatagtgacc attaacgctt gttcgacgcg aggatccggt
    12601 tcctggcctt ctttttctgc ctggcgggag cggtgcagca actcggcctg caatacgttc
    12661 agcgggtcgg tgtaaatatt ccgtagctga atagactctg caatccacgg cagatcggcc
    12721 atcagatggg aatcgttggc aatcgccagc accactttga tgtcttcttc ttgcaggttg
    12781 cgtaactctt tacctaacgg ccacagtgct ttgtctacca gacgttgatc atagtattcc
    12841 gccaaccaca ggtctgcttt ggcgaagacc atctccagca tgccgagacg cgtcgagaag
    12901 aatggccaat cgcggcacat ggcttccagc tcgctctgtt tgccatcttc gaccactttt
    12961 tgcagcgccg tacctgcacc cagccaggcg gggagcatca ggcggttttg cgtccaggca
    13021 aaaatccacg gtatggcgcg cagcgactcg acgccgccgg ttgggcgacg tttcgccgga
    13081 cgtgaaccca acggcagttt gcccaattct tgttccggcg tagcggagcg gaagtaaggc
    13141 acaaaatctt tgttttcacg tacgtagcca cggtagagat cgcaggagat gactgacagt
    13201 tcatccataa tgcgacgcca gctctctttc ggctcaggtg gtggcagcag gttggcttcc
    13261 agaatcgccc cggtgtaaag cgacaggctg ctgacggtga tttctggcag accatattta
    13321 aagcggatca tctcgccctg ttcagtcacg cgcaggccac ctttcaggct tcctggcggt
    13381 tgtgacagca gcgccgcatg ggcaggtgcg ccgccgcgac caatggaacc gccgcgaccg
    13441 tggaacaacg tcagctcaat acccgctttt tcgcaggttt tgattaatgc atcctgtgcc
    13501 tgatattgcg cccaggaagc tgccatcacg cccgcatctt ttgctgagtc ggaatagcca
    13561 atcatcacca tctgtttgcc ctgaatcagg ccgcgatacc agtcgatatt gagcagctgg
    13621 gtcatgacat cgttggcgtt gttcaggtca tcgagggttt caaacagcgg agcaaccggc
    13681 atcgcaaacc cgatacccgc ttctttcagc agcaggtgga cagccagtac gtcggacggc
    13741 gttttcgcca tcgagatcac gtaggcggca atggagcctt gcggcgcttc ggcaatcacc
    13801 tggcaggtat cgagcacttc gcgcgtttcg gcgcttggtt gccagttgcg cggcagaagc
    13861 ggacgtttgg agttcagttc gcggatcagg aacgcctgtt tgtcggcctc tgaccagctt
    13921 tcgtagtcgc cgataccgag gtagcgggtc agctcgccca gcgcttcggt atggcgggtg
    13981 ctctcctgac ggatatcaat acggaccagc ggtacgccga aacatttcac gcggcgcagg
    14041 gtgtcgagca gatcgccgtt ggcgataata cccatgccac acgcctgaag tgactggtag
    14101 caagcgtaga gcggttccca cagttcttcg ttttgtgtca gcagcccttc tggttttggc
    14161 agttcttcgc ctttcaggcg cgcttccagc catgcctgtg tcgccatcag gcgagaacgc
    14221 aggtttttca tcagatagcg atacggttct gcggcacctt cttcgccaac cagcgccagc
    14281 agttcagggg tcgcttcaac catcgacagt tcagaaacca gaacctgaat atctttcagg
    14341 aacagatcgg tggctttcca gcggctgagc agcaggacgt ggcgggtgat atcggcagtg
    14401 acgttcgggt tgccgtcgcg gtcaccgccc atccacgagg taaaacgtac cggaacaaat
    14461 tcgacgggca gtttgtagcc gagattctct tccagttgtt cgttcagttc gcgcaggtaa
    14521 ttcggtacgc cttgccacag gctgttttct actacggcaa agccccattt ggcttcatct
    14581 accgggcttg gacgcagctt acggatttca tcggtatgcc atgactgggc gatcaactgg
    14641 cgcaggcgac gcatcagctg gttgcgttcg tagtcagcga tatctttgtt atcgagctgt
    14701 tttaaacagg cgttcacttc caccattttg tggatcagtg tacgacgggt aatttcggtt
    14761 gggtgagccg tgaggaccag ttccagcgac agcgattcca ctgctttttt gatggtgtct
    14821 tcgctcagtt ccggctggtt tttcagttta cgcagggtgc gggcgatcac ttccgggttg
    14881 ctggcagctt cgcctttcgg cgaaatgctg tggtattgct cggcggtgtt ggccaggttc
    14941 aggaactgac taaacgcacg cgcaacgggc agcagctcgt cgttcgacaa attttgtaag
    15001 gtggtgagca actcctggcg gttagcatca ttgccagcgc gtgaagattt cgacaactta
    15061 cggatagttt ctacgcgttc aagaatgtgt tctcccaacg catccttgat ggtttctccc
    15121 agcactttgc cgagcatact gacattacta cgcaatgcgg aatattgttc gttcatatta
    15181 ccccagacac cccatcttat cgtttgatag ccctgtatcc ttcacgtcgc attggcgcga
    15241 atatgctcgg gctttgcttt tcgtcgtctt ttataaagcc acgtaaaagc ggtgacgtca
    15301 aatgctgcga aatcgcttca gcaaacgaat aaatagcagg aatttacgcc attaaattca
    15361 cgacgcttta aataagcgta acttatggaa atgttaaaaa atcgccatac ataacaccaa
    15421 aggtgtaggt cggataagat gcgcaagcat cgcatccgac attattgcgg caccggagtt
    15481 tggcaacggt gccggatgcg gcgcgagcgc cttatccggc ctacgtttta atgccagcaa
    15541 aaatggtgaa ttacctggat aatcaactca cgggtgggtt taataaaccg cgtctccaga
    15601 tattcatcag gctgatgagc ttgattgatg gaacccggac cgagcaccag cgtcgggcat
    15661 aacgtttgaa taaacggcgc ttcggtacag taattcacca cttcggtttt tgctccgagc
    15721 aatttctcaa ccacttcaac cagttgatga ttcggtgggc attcatagcc agggatcggc
    15781 ggatgcagct cgtcgaccgt cagacgaccc ggccagcgtt cgctcaccgg agccaatgca
    15841 tcgttgagca aaccattaag ttcattgagt gtcatgccag gctgcggacg aatatccata
    15901 tgcaactcac agcaagcgca aatacggtta gaagcgtcgc caccgtgaat atgcccgagg
    15961 ttgagcgtag ggtatggcac ggtaaatgct tcgtagtgat aacgttcttt caggttatcg
    16021 cgcaat
//
LOCUS       DAJTSI010000092        15920 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000092 DAJTSI010000000
VERSION     DAJTSI010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15920
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.092"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            436..1494
                     /gene="citC"
                     /locus_tag="OO327_002650"
     CDS             436..1494
                     /gene="citC"
                     /locus_tag="OO327_002650"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR6019139.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1509..1805
                     /gene="citD"
                     /locus_tag="OO327_002651"
     CDS             1509..1805
                     /gene="citD"
                     /locus_tag="OO327_002651"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR6019140.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1802..2710
                     /gene="citE"
                     /locus_tag="OO327_002652"
     CDS             1802..2710
                     /gene="citE"
                     /locus_tag="OO327_002652"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR6019141.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2721..4253
                     /gene="citF"
                     /locus_tag="OO327_002653"
     CDS             2721..4253
                     /gene="citF"
                     /locus_tag="OO327_002653"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR6019142.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4257..4807
                     /gene="citX"
                     /locus_tag="OO327_002654"
                     /pseudo
     CDS             4257..4807
                     /gene="citX"
                     /locus_tag="OO327_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            4782..5660
                     /gene="citG"
                     /locus_tag="OO327_002655"
     CDS             4782..5660
                     /gene="citG"
                     /locus_tag="OO327_002655"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCR6019143.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTCFLAQI"
     gene            5711..7173
                     /gene="citT"
                     /locus_tag="OO327_002656"
                     /pseudo
     CDS             5711..7173
                     /gene="citT"
                     /locus_tag="OO327_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7287..8093
                     /gene="rna"
                     /locus_tag="OO327_002657"
     CDS             7287..8093
                     /gene="rna"
                     /locus_tag="OO327_002657"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR6019144.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8322..8732
                     /gene="rnk"
                     /locus_tag="OO327_002658"
     CDS             8322..8732
                     /gene="rnk"
                     /locus_tag="OO327_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR6019145.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8963..10201)
                     /locus_tag="OO327_002659"
     CDS             complement(8963..10201)
                     /locus_tag="OO327_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HCR6019146.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNVQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10422..10850
                     /gene="uspG"
                     /locus_tag="OO327_002660"
     CDS             10422..10850
                     /gene="uspG"
                     /locus_tag="OO327_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR6019147.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10971..12536)
                     /gene="ahpF"
                     /locus_tag="OO327_002661"
     CDS             complement(10971..12536)
                     /gene="ahpF"
                     /locus_tag="OO327_002661"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR6019148.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12781..13344)
                     /gene="ahpC"
                     /locus_tag="OO327_002662"
     CDS             complement(12781..13344)
                     /gene="ahpC"
                     /locus_tag="OO327_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR6019149.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13716..14462
                     /gene="dsbG"
                     /locus_tag="OO327_002663"
     CDS             13716..14462
                     /gene="dsbG"
                     /locus_tag="OO327_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR6019150.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14672..15574
                     /locus_tag="OO327_002664"
     CDS             14672..15574
                     /locus_tag="OO327_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6019151.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15721..15920
                     /locus_tag="OO327_002665"
                     /pseudo
     CDS             15721..15920
                     /locus_tag="OO327_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         3952 a         4115 c         3850 g         4003 t
ORIGIN      
        1 ggaaatgcca gtctttggaa aaatgcaaac tgtttattct cgttaagctg caacattgtt
       61 ttccttgcgg gttgtcccga gcctctttga cggatgtaat taaaatgttg aatcaaaata
      121 catagatatt aattatgatc gtggcaaatt ttattcatta aatcaattaa atcacttaaa
      181 cctgcgagtc atttcgtaat tacattaatt aaaaaactta aaaccattaa ataaataaaa
      241 aaccactaac tctatgtgaa ataaatcaaa atttcacgcc gaaatactcc ttaggatgta
      301 tagcaaaagg agaaaaagat atacctcgat cacccccctt tctcccaagt gaaaataaaa
      361 ggttatcagt ttgcaacatt gaacaacatt cgttgcaaat cgataacaac atgcaccttc
      421 aggatactat ttattatgtt cggcaatgat attttcaccc gcgtaaaacg ttcagaaaat
      481 aaaaaaatgg cggaaatcgc ccaattcctg catgaaaatg atttgagcgt tgacaccaca
      541 gtcgaagtat ttattaccgt aacccgcgat gaaaagctta tcgcgtgcgg tggaattgcc
      601 ggaaatatta ttaaatgcgt tgctatcagt gaatccgttc gcggtgaagg actggcgctg
      661 acattagcca ctgaactgat aaacctcgcc tatgagcggc acagcacgca tctgtttatt
      721 tataccaaaa ccgaatacga ggcgctgttc cgccagtgcg gtttttccac gctgacctgc
      781 gtacccggcg tgatggtgct gatggaaaac agcgccacgc gactgaaacg ctatgccgaa
      841 tcgctgaaaa aatttcgtca tccagggaac aagattggct gcattgtgat gaacgccaat
      901 ccctttacga atggtcaccg ttatctgatt caacaagctg cggcacagtg cgactggttg
      961 catctgtttt tagtcaaaga agattcttca cgcttcccct atgaagaccg gctggatctg
     1021 gtgttaaaag gcaccgccga tattccacgc ctgactgtgc atcgcggctc agaatacatc
     1081 atctcccgcg ctacgttccc ttgctacttc attaaagaac agagcgtcat taaccattgt
     1141 tacaccgaaa ttgatctgaa gattttccgt cagtacctcg ctcccgcgct tggtgtaact
     1201 caccgctttg tcggtactga acccttttgt cgcgttaccg cccagtacaa ccaggatatg
     1261 cgctactggc tggaaacgcc gactatctcc gcagcgccca tcgaactggt tgaaattgag
     1321 cggctgcgtt accaggagat gccgatatcc gcttcccggg tacgtcaact gctggcgaaa
     1381 aacgatctca cggctatcgc gccgctggtc cctgcagtca cgctgcatta tttgcagaac
     1441 ctgcttgagc actcccgcca ggacgcggca gctcgtcaaa agacccccgc atgagaaaca
     1501 ggtgaaaaat gaaaataaac cagcccgccg ttgcaggcac ccttgagtct ggggatgtga
     1561 tgatacgcat cgccccactc gatacgcagg atatcgacct gcaaatcaat agcagcgttg
     1621 agaaacagtt tggcgatgca attcgcacca ccattctgga cgttctcgcc cgctacaacg
     1681 tgcgcggcgt acagctgaat gtcgatgaca aaggcgcact ggactgcatt ttacatgcac
     1741 gactggaagc cctgctggca cgcgccagcg gtatcccggc tctgccatgg gaggattgcc
     1801 aatgatttcc gcttcgctgc aacaacgtaa aactcgcacc cgccgcagca tgttgtttgt
     1861 gcctggtgcc aatgccgcga tggtcagcaa ctccttcatc tacccggctg atgccctgat
     1921 gtttgacctc gaagactccg tggcattacg tgaaaaagac accgcccgcc gcatggttta
     1981 tcacgcgctg caacatccgc tgtatcgcga tattgaaacc attgtgcgtg tcaacgcgct
     2041 ggattccgaa tggggtgtta acgacctgga agccgtcgtt cgcggtggtg cggacgttgt
     2101 gcgtctgccg aaaaccgata ccgctcagga tgttctggat attgaaaaag agatcctgcg
     2161 tatagaaaaa gcctgtggtc gtgaacccgg cagcaccggc ctgctggcgg cgattgaatc
     2221 tccgctgggc attacccgcg cagtggaaat cgctcacgct tccgagcgtt tgatcggtat
     2281 cgccctcggt gcagaagact atgtgcgcaa cctgcgtaca gaacgctccc cggaaggaac
     2341 tgaactgctg ttcgcacgct gttccatttt gcaggccgcg cgctctgcgg gtattcaggc
     2401 gttcgatacc gtctattccg acgctaacaa cgaagccgga ttcctgcaag aagccgccca
     2461 catcaaacag ctgggctttg acggcaaatc gctgatcaac ccgcgtcaga ttgatctgct
     2521 gcacaacctc tacgcaccga cccagaaaga agtggatcac gcccaccgcg tcgtagaagc
     2581 cgctgaagcc gccgctcgcg aaggcctcgg cgtggtttcc ctgaacggca agatggtgga
     2641 cggtccggtt atcgatcgcg cccgtctggt gctctcccgt gcagaacttt ccggcatccg
     2701 cgaagaataa ggcaatcaaa atgacgcaga aaattgaaca atctcaacga caagaacggg
     2761 tagcggcctg gaatcgtcgc actgaatgcg atcttgccgc tttccagaac tcaccaaagc
     2821 aaacctacca ggctgaaaaa gcgcgcgatc gcaaactgtg cgccaacctg gaagaagcga
     2881 ttcgtcgctc aggtttacag gacggcatga cggtttcctt ccatcacgct ttccgtggcg
     2941 gtgacctgac cgtcaatatg gtgatggacg tcatcgcgaa gatgggcttt aaaaacctga
     3001 ccctggcgtc cagctccctg agtgattgcc atgcgccgct ggtagaacac attcgccagg
     3061 gcgtggttac ccgcatttat acctctggcc tacgcggtcc actggcggaa gagatctccc
     3121 gtggtctgct ggcggaaccg gtgcagatcc actctcacgg cggtcgtgtg catctggtac
     3181 agagcggcga actgaatatc gacgtggctt tcctcggcgt cccgtcctgt gatgaattcg
     3241 gtaatgccaa cggctacacc ggtaaagcct gctgcggctc cctcggctat gcaatggttg
     3301 atgccgacaa cgcaaaacag gtcgtgatgc ttaccgaaga actgctgcct tatccgcata
     3361 atccggcaag cattgagcaa gatcaggttg atttgatcgt caaagttaat cgcgttggcg
     3421 atgctgcaaa aatcggcgct ggtactaccc gtatgaccac taacccgcgc gaactgctta
     3481 ttgcccgtag cgctgcggat gtgattgtca actctggcta cttcaaagaa ggtttctcca
     3541 tgcaaaccgg caccggcggc gcttcgctgg cggtaacccg tttcctggaa gacaaaatgc
     3601 gtagccgcga tattcgcgcc gacttcgccc tcggcggtat taccgcgacg atggttgatc
     3661 tgcacgaaaa aggtctgatc cgcaaactgc tggatgtgca gagctttgac agccatgctg
     3721 cgcaatcgct ggcccgtaac cccaatcaca tcgaaatcag cgccaaccag tacgctaact
     3781 ggggttcgaa aggcgcatcg gttgatcgtc tcgacgtggt ggtactgagc gcgctggaaa
     3841 ttgacaggca gttcaacgtt aacgtgctga ccggttctga cggcgtactg cgtggcgctt
     3901 ccggtggtca ctgcgatacc gcgattgcct ctgcgctttc catcatcgtc gcgccactgg
     3961 tacgcggtcg tattccgact ctggtggata acgtgctgac ctgcatcacc ccgggctcca
     4021 gtgtcgatat tctggtcaca gaccacggta tcgcagttaa cccggcacgt ccggaactag
     4081 cagaacgtct gcaggaagcg ggcattaaag tggtttccat tgagtggctg cgcgaacgtg
     4141 cgcgtctgct gaccggtgaa ccacagccga ttgaattcac cgaccgcgtc gttgccgttg
     4201 tgcgttaccg cgatggctcg gtgatcgatg ttgtgcatca ggtgaaggaa taagccatgc
     4261 acctgcttcc tgaactcgcc agccaccatg cggtgtcaat tcccgagctg ctcgtcagcc
     4321 gggatgaaag gcaagcacgg caacacgcct ggctcaagca ccatcctgtt ccactggtct
     4381 cctttaccgt ggttgcgcct gggccgatta aagacagcga ggtcacacgc cgaattttta
     4441 atcatcgcgt gacagccttg cgtgccttag ccacaaagca gggctggcaa attcaggagc
     4501 aggctgcact ggtttccgcc agcgggccgg agggcatgtt gagcattgcc gccccggctc
     4561 gcgacctcaa gctcgccacc attgagcttg aacatagtca tcctctcggg cggttatggg
     4621 atatcgatgt cctgacgccc gaaggcgaaa ttctctcccg ccgcgactat tcactgcgcc
     4681 tcgccgctgc ctgttgtgcg agcaaagcgc agccgtctgc gcgcgtggaa aaacccatca
     4741 actgaccgat ttactcaacc gcatggaggc actgctgaac gatgtcgatg cctgcaacgt
     4801 caactaaaac cacaaagctt gcgacgtcat taatcgatga gtacgccctg ctgggctggc
     4861 gcgccatgct gactgaagtc aatctgtcac cgaaaccagg cctcgtggat cgcattaact
     4921 gcggtgcgca caaagatatg gcgctggaag atttccaccg cagcgcgctg gcgattcagg
     4981 gctggttacc ccgtttcatt gaatttggtg cctgtagtgc agaaatggca ccggaagcgg
     5041 tactccacgg attacgccca attggtatgg cttgcgaagg tgatatgttc cgcgccactg
     5101 cgggcgtaaa cacgcataaa ggcagcattt tttctttagg gctgctatgt gcggcaattg
     5161 gccgtttgct tcagctcaac cagccggtta cgccaacaac cgtttgttct acggcggcaa
     5221 gtttctgccg tggcctgacc gatcgcgaac tgcgtaccaa taattcacga ctgacggcag
     5281 gtcaacggtt gtaccagcag cttggcctta ccggcgcacg cggtgaagcc gaagcaggtt
     5341 atccactggt gatcaaccac gccttgccgc attacctcac tctgctggat caggggttag
     5401 atcctgaact ggcattgctc gataccttgc tcctactgat ggctaccaac ggcgatacca
     5461 acgttgcatc gcgcggtggc gaggggggcc tgcgctggct acagcgcgag gcgcaaacat
     5521 tattgcaaaa agggggcatt cgaaccccca ccgatctcga ttatctccgg cagttcgaca
     5581 gggagtgtat cgaacgaaat ctcagtccag gcggcagtgc cgacctgctg atccttacct
     5641 gttttttagc acagatttaa ttatttaagc acttgataaa tttggaaata ttaattttcg
     5701 gagaacccgt atgtctttag caaaagataa tatatggaaa ctattggccc cactggtggt
     5761 gatgggtgtc atgtttctta tccctgtccc cgacggtatg ccgccgcagg catggcatta
     5821 cttcgctgtg tttgtggcaa tgattgtcgg catgatcctc gagccaattc cggcaacagc
     5881 gatcagtttt attgcggtta ctatttgcgt tattggcagt aattacctgc tctttgatgc
     5941 caaagaatta gctgacccag cgtttaatgc gcaaaaacag gcgctgaaat ggggcctggc
     6001 tggtttttcc agcaccacgg tatggctggt atttggcgca tttatttttg cattagggta
     6061 tgaagtttcc gggttaggtc gtcgcattgc ccttttcctg gtgaaattca tggacaaacg
     6121 cacgctgacg ttgggttatg cgattgtcat tatcgacatt ctgctggcac cgtttacacc
     6181 gtccaacacc gcgcgtaccg ggggtacggt ttttccggtc attaaaaacc tgccgccgtt
     6241 gtttaaatca ttcccgaacg atccgtccgc gcgtcgtatt ggcggctatt tgatgtggat
     6301 gatggtcatt agtaccagtc tgagttcgtc catgtttgtc accggtgcgg accaaacgtg
     6361 ctgggtctgg agttcgtcag caaaattacc ggtatccaga ttagctggtt gcagtggttc
     6421 ctctgcttcc tgccggttgg ggttatcttg cttatcattg cgccgtggct ttcctacgtg
     6481 ctgtacaaac cggaaatcac acacagtgaa gaagtggcaa cctgggcggg tgatgaacta
     6541 aaaaccatgg gtgcgctgac acgcagagag tggacgctga ttggccttgt attgctcagc
     6601 ttaggtttgt gggtatttgg cagtgaagtc attaatgcta ctgcggttgg tctgctggca
     6661 gtttcgctaa tgctggctct gcacgttgtg ccgtggaaag acattacccg ctataacagc
     6721 gcatggaaca cgctggtcaa cctggcaact ctggttgtga tggctaacgg cctgactcgt
     6781 tctggtttta ttgactggtt cgccggtacc atgagtacgc acctggaagg attctcacca
     6841 aacgcaacgg tgattgtact ggttctggtg ttctactttg cacactacct gtttgccagc
     6901 ctgtctgcgc acaccgcaac catgctgccg gttattctgg ccgtcggtaa aggtattccg
     6961 ggcgtaccaa tggaacaact gtgtatcctg ctggtgctgt ctatcggtat catgggctgt
     7021 ctgacgccgt atgcaaccgg tcctggggtg attatttacg gctgtggcta tgtgaaatca
     7081 aaagattact ggcgtcttgg cgcaatcttc ggggtgattt acatctctat gttgctgttg
     7141 gttggctggc cgattctcgc catgtggaac taatacgttc gccaaacgct gaataaatca
     7201 tcacgcccgc caggtaagcc cacctggcgg gcttttttat gatttaatag atagtattca
     7261 gtgtatgtat gagttccaca cccattatga aagcattctg ccgtaacgcc gcgttgctcg
     7321 cggtttctct gcttccctta tcttctgcca acgccgtagc gttgcaggca aaacagtatg
     7381 gcgattttga tcgctatgtc ctggccctct cctggcaaac cggattttgc cagagtcaac
     7441 acgatcgaaa tcgtaacgaa cgagatgaat gtcgcctgca aaccgaaacg accaacaaaa
     7501 ctgattttct gaccgtacat ggtctgtggc caggattgcc taaatcggtt gctgcccgtg
     7561 gtgttgatga acgccgctgg atgcgcttcg gttgcgctac tcgcccaatc ccgaatctac
     7621 cagaagcacg cgccagccga atgtgttcat cgccggaaac cgggttatca ctggaaacgg
     7681 ccgctaaact aagtgaagtc atgccaggag ctggcggacg ttcctgcctg gaacgctacg
     7741 aatatgccaa acacggtgcc tgctttggtt ttgatccgga cgcatacttc ggtacgatgg
     7801 tacgcctgaa tcaagaaatt aaagagagcg aagccggaaa attccttgcg gataattacg
     7861 gtaaaacagt gagccgccgt gactttgacg ccgcctttgc caaaagctgg ggaaaagaga
     7921 acgtgaaagc agttaagcta acgtgccagg gtaaccctgc gtatttgact gaaattcaga
     7981 tctcgatcaa agctgacgcc atcaacgctc cgctttctgc aaactcattc ttgccacaac
     8041 ctcacccagg taactgtggc aaaacctttg tgattgataa agcgggttat taatcctctg
     8101 atttacaaaa cacgctaatt cgtcttccat aaccatgccg attaagaatt atacataatg
     8161 cgctgcattg aggaattccc tgaatgtgac gcaaatcact tcaaagagtg agcaacagtc
     8221 agtatcatca tgtgagttaa accctcgccg cctgacggtg agggttttct tttgggtatg
     8281 tttatctgcg acactcgcag taccgacgac atggagtaaa aatgtccaga ccaactatca
     8341 tcattaacga cctggatgcc gaacgcatcg atattctgct ggagcaaccc gcctatgctg
     8401 gtttgccaat cgccgacgcg ttaaacgcag agttggatcg cgcccaaatg tgttcgccag
     8461 aagagatgcc acacgacgtg gtgacaatga acagccgggt taaattccgc aatcttagcg
     8521 atggcgaagt gcgtgtgcgc acgctggtgt atccggcaaa aatgaccgat agcaatactc
     8581 agctttccgt tatggctccg gtaggtgccg cactgctggg gctgcgcgtt ggcgattcca
     8641 ttcactggga acttccgggc ggcgttgcaa cccaccttga agtgctggaa ctcgaatacc
     8701 agccagaagc tgctggcgac tacctgcttt aatcccatct gcacaagcga taactcgctt
     8761 tgcccagagg atgcactcgc atcctcttta cgcaaaatca gcaatttttc ctggttctca
     8821 ctgttcactt ttgctgcaaa attattccgt ttcgtttgtg ggaataacca tggcagaagc
     8881 attttacatc ctgataggat ttctgattat ggcggcgatt atcgtcatgg ctgtccttta
     8941 cctcgaaaac cactcctgac gatcatattg ttccccccgg catcgcattc accagacctg
     9001 aaaccgcctt ctgcgccgcc tctgcgcttt gtgcaccggg taccagaatc accttacggc
     9061 actcctcttc acgtttttcg aaaatctcat atccccgggc ggcctcttca aacggcatat
     9121 agtgggtaac aatttcttct ggtttcagta atcctttctc aattaacggt aataattctc
     9181 ccagccatgc gtgaacgtgg gtctgtccca ttttaaacgt caacccttta tcaaaggcgt
     9241 cgccaaacag gaaaccgtga ataaatccag cgtagacgcc cggtacgcta acaatgccgc
     9301 cacgcctgac cgccgcaata cactggcgca acgctttacc gctgctgccc tccagtttca
     9361 ggttagtcag caccgtttcc gtggtgctgc ctttcgcttc aaaaccgacg gcgtctatta
     9421 ctgcatccac gccccggtga cctgccgttt gttcaataat tgactgtgcc ggatcgctat
     9481 cttcatcgaa attaatcggg atcgcgccgt agcggtcagc ggcgaaacgc aagcggtagg
     9541 gatgatgatc aacaacaaaa atctgttccg caccgagcaa ccgtgcacag gcgattgtca
     9601 acaatcccac aggaccagca ccatagactg caacgcttga accttgttgg atctgcacat
     9661 tttttgctgc ctgccatgcc gttggcagaa tatcagaaag gaaaagggct ttatcatctg
     9721 aaagcaaagg cggtacttta aacggcccca cattcccttt cgggacgcgg acatattccg
     9781 cctgcccgcc aggaacgcca ccatacaggt gactataacc aaacaatgcc gcgggagcag
     9841 gtatctgttt tttattgagc gcagcgcctt tacccgcatt ggtattttcg caggcggcat
     9901 attgctgtaa tcgacagaaa aaacagtcgc cacaagcaat gacgaacgga attaccaccc
     9961 ggtcgccttt ttgcaaattt tttacgtcct tcccggtttc aacgacttcc cccataaatt
    10021 catgaccaaa aatatcgcca tgtttaacct ggggtatttt gcctcgataa agatggaggt
    10081 cagagccaca gatcgccgtt accgtaatac gcagaataat atcatctgcc tgttcaatgc
    10141 ccggatcggg aacattttct acctgaacgt gatgtgggcc gtgataagtc aatgctttca
    10201 tgcaacctcc agagacagtt caaaaaagag tcagtcttta agggtagcta tcacagcggg
    10261 aaaacgggcg gtaccgcagg ttttgggaga attcctagtg ttcagtgaga atatattaat
    10321 cacagaaacc gttcgtaagg aaaaagaacc agacaaatat cagggataaa tattataatc
    10381 atggagaatg atttgttcat gattaacagg gagaaagggt tatgtataag gcaatcatta
    10441 tgccagttga tgtatttgaa atggaattga gcgacaaagc tgttcgccac gctgaattcc
    10501 tcgcccagga tgacggagtt attcatctac ttcacgtact acccgggtca gccagcctga
    10561 gcctgcaccg ttttgccgct gatgtgcgtc gttttgaaga gcatctgcaa catgaagcag
    10621 aagaacgtct gcaaacgatg gtcagccact tcaccatcga tccttcccgc attaaacaac
    10681 atgtccgttt tggtagcgtg cgggatgaag tcaatgagtt ggcagaagaa ctgggggctg
    10741 atgttgtagt tatcggttct cgcaacccat cgatttcgac ccatctgtta ggttctaacg
    10801 cctcgagcgt gatccgccac gccaatctgc ctgtactggt cgttcgctaa cagataaaag
    10861 tatttatgcc gccctgcctg gaacatcccg gcagggcttt ttaatgccat aaaaaagccg
    10921 ccaggtttga ccccggcggc taagcaattg caggtgaatc ttacttcttc ttatgcagtt
    10981 ttggtgcgaa tcaggtagtc aaaagcactc agagaggctt tggcaccttc gccagtggcg
    11041 atgatgatct gcttgtacgg aaccgtcgta cagtcacccg ctgcgaacac gcctttgacg
    11101 ttggtttcac atttcgcatc aatgataatc tcgcccatgc ggttacgttc gactgcgcct
    11161 tccagccagt tggtgttcgg cagcagacca atctggacaa aaataccggc cagttcgatg
    11221 ttgtgaatat tgccgctgac acgatctcga tattccagac caacgacttt gctgccgtcg
    11281 cctttcactt ccgtggtttg cgcattcaga ataatgtcga cgtttttcag gctgcgcagt
    11341 ttgtcctgca gaacctggtc ggctttcatt tctggcgcaa attccagcag cgttacgtgc
    11401 tcaacgatac ccgccaggtc gattgccgct tccacgccgg agttaccgcc gccgataacc
    11461 gctacgcgtt tgcctttaaa cagcgggccg tcgcagtgcg ggcagtaggt cacgccttta
    11521 gtgcgatact gatcttcgcc cggaacgttc atgttgcgcc attttgcacc cgtcgccacg
    11581 ataatgctgc gtgctttcag taccgcgcca gaagctgttt caatctggtg tagaccacct
    11641 tcaaccgctg ccgggatcag tttgctggcg ctctggctgt cgatcacatc aacgtcgtat
    11701 tcatcaacgt gaactttcag tgcgcctgcc aacttctgcc cttcagtctt cggtacagaa
    11761 atgtagtttt cgatatcaac ggtatcgagg atctgaccac caaaacgttc gcccatcaga
    11821 ccggtacgga tgcctttacg tgcggagtaa attgctgccg ctgcacccgc cgggccggaa
    11881 ccaacgatta atacgtcata agcatcacgc ttgttcagct cttctgccgc acgattttcc
    11941 gcgccagtat caattttggc aacgatttca gtcaacgtca tgcggccctg accaaactct
    12001 ttcccgttta cgaacactgc cggaacgccc atcacgttgc gatcggtgat ttcgttctgg
    12061 aaggtgccgc cgtcaattgc agtgtgcttg atgcgcgggt tcaatacgct catcaggttc
    12121 agcgcctgca ccacgtccgg gcagttatgg caagagagcg agtaataggt ttcgaattca
    12181 aaatcaccgt caatatggcg aatctgctcc agcagagact gcgcttcttt cgacggatga
    12241 ccaccggtcc acagcaacgc cagtaccagc gaggtgaact cgtggcccag cggggagcct
    12301 gcaaaacgtg gtccctggtt ggaacctggg ttggtgatca ggaaagacgg cttacgcact
    12361 ggcaagctgt tatcttcttt aaaggtgact ttgtctgaca gttctgcgat ttcagccaac
    12421 agttccttga tttctgccga tttagcgctg tcatccagcg tggcaattaa ctcaacaggc
    12481 ttggtcaatt tctcaaggta agccttgagt tgagttttca tatttgtgtc gagcatgttt
    12541 atctcctggg ctttaaacat catcatgcaa gcggcctctg cggctacctg gatgcagctt
    12601 gcatcatggt gcggggaaga aaatcgggtg ccgcagcacc cgaagaatta ggtgaatttc
    12661 cgaagtattt cgcgtcagag caaggcgact agcctgtgaa tccccgggag cttacacaag
    12721 taagtgaccg gggtgagcgg gcgacgccaa cgccgctatg gcgtgaaaga cgaaggaaat
    12781 ttagatttta ccaaccaggt ccagagacgg agccagagtt gcttcacctt ctttccattt
    12841 agccgggcaa acttcacctg ggtgagaagc tacgtactga gctgctttga ttttacgcag
    12901 cagatcagac gcgtcacggc caatgccttc agcggtaact tcgattgcct ggatgatacc
    12961 ctgcgggtca acaacgaagg tcgcacggtc agccagacct tcatcttcac gcatgttgtc
    13021 gaagttacgg gtcagggcgc cagtcgggtc gccgatcatc gcatatttga ttttagcgat
    13081 ggtttcagag ctgctgtgcc atgctttgtg ggtgaagtga gtatcggtag atactgcgta
    13141 tacgtctacg cccagtttct gcagttcttc gtagtggtca gcaacgtcac ccagttcggt
    13201 cgggcatacg aaagtaaagt cagccgggta gaagaagaag acgctccagc ggccttcggt
    13261 atctttttcg gtgatttcga tgaattcgcc gtttttgaat gcctggtttt taaaaggttt
    13321 aattttggtg ttaatcaagg acatctatac ttcctccgtg ttttcgatga gatgtaaggt
    13381 aaccaatttt tgccgattcg gctaatgcgt ttccattatc aaatcgataa gttttacctt
    13441 acaaccttcg taagacaact ttgtgaactt tgcaccgcca gcggcgataa aaagtaaaat
    13501 ggcccccttt ccaggtgacc gtgtaatctg aactaccctt tggtatcttc agcgcctgcc
    13561 tgcaggctga ggtgttgccc ttacaaatgc aacaacgaca tggattacaa caccctcata
    13621 aacaaagggc aatcacctga tctaagctct tacctatgac agtgataggt tatgcctttt
    13681 actcgacttt tgcactgact gaaaagaaca aattaatgtt aaaaaagata cttttactgg
    13741 ctctgcttcc tgcaatcgcc ttcgcagagg aacttccttc cccagtaaaa gcgattgaaa
    13801 aacagggcat tacaatcatc aaaacgttcg atgcccccgg aggaatgaaa ggttatctcg
    13861 gaaagtatca ggatatgggc gtcaccatct acctgactcc cgatggtaag cacgctatct
    13921 ctggttacat gtacaacgag aaaggtgaaa acctgagtaa cacacttatc gaaaaagaaa
    13981 tttacgcacc agccggacgc gaaatgtggc aacggatgga acaatcccac tggctcctcg
    14041 acggtaaaaa agatgcgccg gtcattgtct acgtcttctc cgatccgttc tgcccatatt
    14101 gtaaacagtt ctggcagcag gcgcgcccgt gggtagattc tggcaaagtg caattaagaa
    14161 cattgttggt tggggttatc aagccagaaa gcccggcgac agcagcggca attcttgcct
    14221 ccaaagatcc cgcaaaaacc tggcaacaat atgaagcctc tggttgcaag cttaagctaa
    14281 acgtgcctgc aaacgtaagt acagagcaaa tgaaagtgtt aagtgacaat gagaaactga
    14341 tggacgatct gggggcaaat gtcacgccgg ctatctatta catgagtaag gaaaatacgc
    14401 tgcaacaggc cgtggggttg cccgatcaga aaacgcttaa tatcattatg ggaaataaat
    14461 aaaaggtaat tcatggatgg ttgaactata tccctgcttt tcaaccatcc taaaaaagca
    14521 tgaatttctc gctctaccct aagtggctgt gctattttag aaaaacttaa tcctgagtta
    14581 agcaaaaaaa cataatcaat aaaaatacat acgcaaaaaa cattgattaa gtgaatatac
    14641 catggaagaa aaatataaac cggagtagtg tatggccaat ctctacgact tgaaaaagtt
    14701 cgacttaaat cttcttgtca tatttgagtg tatttaccag catttaagca ttagcaaagc
    14761 tgctgaatca ctatatatca ccccatcagc cgtgagccag tcgttacaac gcttacgtgc
    14821 gcaatttaat gaccctttat tcattcgctc aggaaaaggt atcgcgccga cgacaacagg
    14881 gttgaatctg catcaccatc ttgagaaaaa cctcaaaggc cttgaacaaa caattaatat
    14941 agtcaataag tccgaactaa agaaaaactt tattatctac ggccctcagc tcatttcttg
    15001 ctctaataat agtatgctga tccgctgcct acggcaagat gcctctgtcg agattgagtg
    15061 ccatgatatc ctcatgtcag cagagaatgc tgaagaactg ttggttcacc gcaaagccga
    15121 tctggtgatc acccaaatgc cagtgatcag ccgttccgtc atttgcatgc cattgcatac
    15181 aattcgcaat actctaattt gtagtaataa gcatccgcga attactgaca atagcactta
    15241 tgaacaaatt atggcagaag agttcaccca attaatagcc aaatcagcag gagttgatga
    15301 tattcaaatg gaaatagacg agaagtttat gaatcgcaaa atttcatttc gtggttcctc
    15361 tttgctgaca ataattaata gcattgcagt tacagattta ctgggaatag tcccctatga
    15421 actttataac taccaccgtg actttcttaa tttaaaagag ataaaactag aacatccgtt
    15481 accttcaata aagctttaca tttcctataa taaatcttct ttaaataatt tggtgttctc
    15541 ccgatttatt gaccgtctaa atgagagttt ttgatatata actatagaca gctactataa
    15601 ctttcatcta tttattcaca gcgcgaatga aaaataaaac actctgcttt acagtgttta
    15661 ctttataatt aacagtctta tctcactgtt caccctgtaa cgttcagaga gcgtcttcag
    15721 atgtctattt ataaaattcc tcttccgctc aatattctgg aagctgcacg agagcgtatt
    15781 acctagacac tcaacaccct accccgcgtt tgtgtctcat tttctggcgg caaagactct
    15841 ggtttaatgc tgcatctgac agccgaactt gcccgacaaa tgggcaaaaa aatctgcgtt
    15901 ttgtttatca actgggaggc
//
LOCUS       DAJTSI010000093        15821 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000093 DAJTSI010000000
VERSION     DAJTSI010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15821
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.093"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..353)
                     /gene="mukE"
                     /locus_tag="OO327_002666"
     CDS             complement(<1..353)
                     /gene="mukE"
                     /locus_tag="OO327_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR6019152.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQ"
     gene            complement(334..1656)
                     /gene="mukF"
                     /locus_tag="OO327_002667"
     CDS             complement(334..1656)
                     /gene="mukF"
                     /locus_tag="OO327_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR6019153.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(1653..2438)
                     /gene="cmoM"
                     /locus_tag="OO327_002668"
     CDS             complement(1653..2438)
                     /gene="cmoM"
                     /locus_tag="OO327_002668"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR6019154.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            2574..3353
                     /gene="elyC"
                     /locus_tag="OO327_002669"
     CDS             2574..3353
                     /gene="elyC"
                     /locus_tag="OO327_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR6019155.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(3330..4223)
                     /locus_tag="OO327_002670"
     CDS             complement(3330..4223)
                     /locus_tag="OO327_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR6019156.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(4377..5123)
                     /gene="kdsB"
                     /locus_tag="OO327_002671"
     CDS             complement(4377..5123)
                     /gene="kdsB"
                     /locus_tag="OO327_002671"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR6019157.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(5120..5302)
                     /gene="ycaR"
                     /locus_tag="OO327_002672"
     CDS             complement(5120..5302)
                     /gene="ycaR"
                     /locus_tag="OO327_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR6019158.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(5354..6586)
                     /locus_tag="OO327_002673"
     CDS             complement(5354..6586)
                     /locus_tag="OO327_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR6019159.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(6623..7609)
                     /gene="lpxK"
                     /locus_tag="OO327_002674"
     CDS             complement(6623..7609)
                     /gene="lpxK"
                     /locus_tag="OO327_002674"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR6019160.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHEDVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(7606..9354)
                     /gene="msbA"
                     /locus_tag="OO327_002675"
     CDS             complement(7606..9354)
                     /gene="msbA"
                     /locus_tag="OO327_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR6019161.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(9391..11650)
                     /locus_tag="OO327_002676"
                     /pseudo
     CDS             complement(9391..11650)
                     /locus_tag="OO327_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(11857..12141)
                     /gene="ihfB"
                     /locus_tag="OO327_002677"
     CDS             complement(11857..12141)
                     /gene="ihfB"
                     /locus_tag="OO327_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR6019162.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(12301..13974)
                     /gene="rpsA"
                     /locus_tag="OO327_002678"
     CDS             complement(12301..13974)
                     /gene="rpsA"
                     /locus_tag="OO327_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR6019163.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGAAVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(14085..14768)
                     /gene="cmk"
                     /locus_tag="OO327_002679"
     CDS             complement(14085..14768)
                     /gene="cmk"
                     /locus_tag="OO327_002679"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR6019164.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(14941..15705)
                     /gene="ycaL"
                     /locus_tag="OO327_002680"
     CDS             complement(14941..15705)
                     /gene="ycaL"
                     /locus_tag="OO327_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR6019165.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         3958 a         4287 c         3776 g         3800 t
ORIGIN      
        1 tgctgggtga aaatcccctc attcgccagc cgttccgggc tgagatagag ataacagagg
       61 attttcccga ccatcatatc cagttccgac aagacggaac gcgggataag agtggtggaa
      121 cgtgggcgta aatagaagaa cccttccggt gcgcgaataa gctcaacgtt ataacgcgcg
      181 taaaactctt ccagatattc ctgaaaatcc atcaggaatg catgattatc cagttcgtcg
      241 aggccaatat ggcggcctga acgtaaggcg ctgtccagcg ccggaaataa cggattcgcc
      301 agcgcctgcg ccagcttaac cggcatcact tgttcaatat ttgtcgatga catgcgcctg
      361 taccttggct ccgtaatcat taatcggctg ccatttcgct ggcagtccgg tgaaatctgc
      421 ttgcgctacg ccaagacgta ccgcctgatc aataacaata cgcgcaacgt caaagtgacg
      481 tgcacgcgga tactgtgaca gatattcgcg taccaccaga ccaagatcca gcggcacttg
      541 tctggttttg tacacggcaa gttgttcttc gatgatcgcc gccagctgtt cgcggatctc
      601 gttaaactct tcgtattcca gatcctcagg aagttcccca gtcacttctt catcgcgcag
      661 tgccatctct tcgtcacgca tatccagcag acgatcggca ttggcataag ttagcgccca
      721 cggctcatca aaataggttt gtaccgactg acgtaaccgc tgagcaaaga cgcggttttt
      781 atccatatcg atcgcggtac gaataaattt gtgtacgtgg cggtcgtagc caatccacaa
      841 gtcgatggat tgctggcccc aactgataat acgatcgagt ttgctctgca gatcgaacac
      901 cagacgatcg acgaaatgca gatcgtcatg ggtcatcgtc gcatcctgaa tgcgcaacag
      961 attagcctgc aatttgtcgc ctgccgcttc cagcgtatcc tgcaattcac gcagcgttcc
     1021 ggaagtttcc gaaagcaaca attcacagct ggaaatcgcc gcccgccagt ctttgttcag
     1081 caactgggcg atatcgtcct tcacctgctg ctgctgttcg tccatcagac gttgcgtcag
     1141 gtcgatactg tcgaaaattt ctgctaccga atatttcagt ggcgcataga cattacggtg
     1201 ccagtgaaat tcatcaccgc cctcttcggc ggcatctgct gcgcgtttga gctcacccgc
     1261 cacaatcgac aactgcatag aaagacgcag cgtagaaaac tcgcgctgac ggatgtagta
     1321 gtcagtaatg ccgatgccga gcggcgtcag acggtaaatt gcgttccctt ccgcctgctc
     1381 gctggtaaag cggttcagca gacgttgacg caccatgtcg ttgatcgcgt tattggcgcg
     1441 cacgccgatg gtttcgctgg tttgctcaaa cgcatcactc acatggcgga atgcatccac
     1501 cagctcgcct tcactcatct caccatccag acgctcgccg ttcagcgtgg caaccgccag
     1561 cagaaaagag agtcggtcta ccggcagcga gatggagaag tcattttttc tggcccaggc
     1621 aaccagttcg gggactgtct gggaaaattc actcatactt tatccttgct ctgcggtttg
     1681 cgcgcggtga catgaatata acgccctagg gtaatatacg gttcctgacg gcaataacgc
     1741 gtttctaatt caagtaatgc ttcatagcag tcgcgctgct ggtacttctc gcgcagataa
     1801 tcatgaaaca cgcgaacgcc tgtcttaccc ataatttgcc aaccagcttc ttccagccac
     1861 agataaacct gcgccgggtc gcgtggataa tctggcgaaa gcgtccgttt tttctttttc
     1921 ggcattcccg cctgcacgta atcaaaattc ccggcgacca tgttatgcat caacaaacca
     1981 tgcgcattgt agaacattaa cgacaacact ccgcctggac gcaacactga ccacagggtc
     2041 tgcaatacac tgcggggatc ggccacccac tcgagcaccg catggaacaa tatcagatca
     2101 acgggcgttt ccaaatgcga agcaacatcc tgagcggcgc aatgtataaa ttgcatgttg
     2161 tcgctcacac cttttgcttc tgccgcctgt tttgcgcggt cgatcatctg cgcagaaaga
     2221 tcgcataaaa taacctgatg cccgcgctcg gccattttga ttgcggtctg cccttctccg
     2281 ccgccagcat ccagcacacg cagtttttgc gggcccattt ccgccagcac gcgatcgaga
     2341 tcctgccaca gaatagcctg tcgaagctgc cctttggtgg tgccgtaaat gttacgggaa
     2401 aacttttccg caatatcatc aaaattgcga tcctgcattg ctttctccac cggttggcta
     2461 agacgttttc tgataaagac gctattttgt cacagccacg gcgagaatga acccgtctgg
     2521 tgtaatatcg ctagcgatcc cttgctatat ggttaaaaaa ggaaccagaa aggatgcttt
     2581 ttacactgaa aaaagtgatt ggcaatatgt tgctgccact tccgctgatg ttgttaatta
     2641 ttggcgctgg cctggcgctg ctgtggttta gccgttttca aaaaaccggg aagattttta
     2701 ttagcattgg gtggctggca ctcttgttat tgagtttgca accggtagcc gaccgcctgc
     2761 tgcgtcccat cgaaagcacc tatccgacat ggaataattc gcaaaaagtg gactatatcg
     2821 tggtgctcgg aggtggttat acctggaacc cgcagtgggc accgagctct aatttaatca
     2881 ataacagtct gccacgcctg aatgagggta tccgtttatg gcgcgaaaat ccgggttcaa
     2941 aactgatctt cacgggaggc gtagcaaaaa ccaatacggt gagtacagcg gaagtaggtg
     3001 ccagagttgc gcaatcgctg ggcgtaccgc gcgagcaaat tatcaccctg gatttgccaa
     3061 aagataccga agaagaagct gcggcagtga aacaggcgat tggtgacgcc cctttcctgc
     3121 tggtgacctc cgcatcacat ctgccgcggg cgatgatctt ttttcagcag gaagggttaa
     3181 atccgctacc agccccggcg aatcagttgg ccatcgactc gccgcttaat ccgtgggaac
     3241 gggcaatccc ttctccggtc tggctgatgc atagcgatcg tgttggctat gaaacgcttg
     3301 gccgcatctg gcagtggctg aaaggtccgt caggcgagcc acggcaagag tgattttgat
     3361 gccagatcga agttgcgccg actaaatcgt ccggtattaa ccagttgcgc aacttcatcc
     3421 cataacacat acaaccaacg ccgccagatg aacgactccg ccactggcgc gcgttgcagg
     3481 taactccaga gcaaatcttc cgccagagaa ttatccatta gtcggaacag ttcgtattct
     3541 cgcggtgccc aaagcattag tcccggcccg accatcgcca gtaactgatc gctgcgcgaa
     3601 tctttcagca tgctgcgtaa acagaagtta ccgtgaatca acacacaatt gtcgttaaag
     3661 ccttcaaata atgccgggag acattcgcga gtgcgaaaca ggatccgctt atcctgcatc
     3721 gtcagaccgg tgttattgaa ctgattgagc gtggtccata gcacttcaac atgttgccgg
     3781 taccatgagg gccagaaatt ttcctgagta ttgtcgaccg cgccgacgca accgcgactg
     3841 tcctgacggt gccaggccag taaggcttca acgatttggt ctttgagctg ttcccagcgt
     3901 tctggtgttc gggctggtgc ctccaccgaa acaccacgca tccgctccag tagcaggaca
     3961 tcagggccgg ggtgttcttc atgcgtcatc acaccataaa tagtcggcat acggacagtc
     4021 ccacttcttg ccagcatggt ggttttccat gccagttgtc gggcttttcc gggcgtacta
     4081 aagcttcttg ccattaacgg cattgggttt ccctggctgt catacaaagc ccacaacgcc
     4141 gtatccgctt tttcattgac gcactcaata cgactgagtt tttcgcccag taaatggctt
     4201 aattcggcac gcagctgttc cattcagatt accctcataa aagctactgc tttcataatg
     4261 agcgccaata taaaagatgt cacctgaatg agatcaattg ttgaaaagaa aaattgagga
     4321 aaatcgtaac ggtacgacac tcctcccaaa attggctgaa gtgtcgtgaa gtgaaattag
     4381 cgcatttcag cgcgaacgcg ctcaagatct tcaggggtat ccacacctgt gccaggaact
     4441 tcctgagcaa cagcaacatg gattttttcg ccgtaccaca gaacacgaag ctgctctaac
     4501 atttcgatgt gttctaacgg acttgcctgc cagttgacgt aacgacggat aaagcctgca
     4561 cggtagccat aaataccaag atgacgcagg aagttatcgc caacggtttc aaggccttct
     4621 gcaaaacgat cacgatccca aggaatggtg gcacgtgaga agtacagtgc atacccttca
     4681 gcgtcgagaa ccactttcac cgcattcggg ttaaacgctt cttccgcatt gtggattggc
     4741 accgccagag tcgccatacc cacctgacgc tgagcgaggt tatcagcaac ctgacgaatg
     4801 attgtcgcag ggatcatcgg ttcatcaccc tgcacattaa cgatcaccgt gtcgtcgctg
     4861 aatgcgcatt tttcgacaac ttccgccaga cgttctgttc ctgactgatg atcggcgcgc
     4921 gtcatacata cttcaccgcc agcggcttca acggcgcggg caacatcatc atgatcggtt
     4981 gccacgatga tgcgctcggc acctgattca cgcgcgcgtt caagaacatg aacaatcatg
     5041 ggtttgccgt taatatcaac caatggttta ccgggcagac gcgtggacgc gtagcgcgcg
     5101 ggaataatga ccacaaaact catgatttac tctcatcagc agtcagcacg cgggcttccg
     5161 tttccagcaa cactggaatg ccatcgcgga gcgggaaagc caggttatcc agtttgcaaa
     5221 taagctcttg tttttcctgg ttataccaaa gttttccgtt gcaaaccggg caggcaatga
     5281 tttcaagcaa acgatgatcc atagttcctc cagatggatc gggttatgaa tgcataaatc
     5341 ttatcataat catttatgcg acggggtcta tttcccagcc agtgcggcaa tcagtaaaga
     5401 gaccttgcgg gcagcgtcct aatgtcaccc gcgttgcctg ctgccagctc gcgaaatcag
     5461 taatcgcctg acgtaaccct ttttgcagca ttgtcgttgg tttaatgcct tcctgtaacc
     5521 acagagagat aacttcaagg atgcctgtct ggcgatgcat tttggcatcc attcgcccaa
     5581 ctaattgccc acgatgtaat aatggcagaa caaaatagcc atactggcgt ttcggcgctg
     5641 gggtatagca ctccagccgg tagctaaaat caaaaagctg ctctgcgcgt ttgcgatccc
     5701 agacaacagg atcaaaaggc gaaagtaccg cgctgtgcgt tgcagtgagc tttccggcca
     5761 gcgcacgctc gagtagcggc agcaaatcag catgcagcca aagattgccc aatttttcaa
     5821 catgcacagc aatgatttgc tgctgttcag ccctcgcttc gcgccacgcc gccagtgccg
     5881 gacgtttcag ccgatagtaa tctgccagcc actgttcgcg gaatattccc aggctacgcg
     5941 cactgttatc cagcatgatg atttctgctt ctgtttgcga aacgagatcg cgctcatcat
     6001 cccagtcagg catgacacgg tgggttaaat cataaacgcg ctggaagttg cgccgttcaa
     6061 tcaccatcac ctttccggca gtaaataaac cttccagatg ccgtttatgc ggcttccatt
     6121 cccaccagcc gcttgcacct ttacgaggat gctcaaaatc ggctgaacgt accggccctt
     6181 tatcatgaat atgctgaatt aactgtgcaa tttccgcctc atgttcctgc atccaggcgt
     6241 ctttgtattt ccagcccatt ttttcaggtg ccagcatgcg gtggcgaata agacgaaagt
     6301 cgctacgcgg cataaagcag gcttcatgcg cccagtattc cattaattcg ccacgcgcca
     6361 gagactcatc cagccactgg gcaggataat ttcccagacg actgaaaagc accagatatg
     6421 gactacgggc aacaatattg atggtatcga tttgcagcaa ggacatgcgg gagatcgttg
     6481 ccggaatatc ctccaacgac gctcgacggc ggggtttgtt taacaggcct tgtgcggcaa
     6541 ggtgaagatt acgcgcatca gcaagggaga ggtgcggcag cgacattcat gactccatca
     6601 atcgaacgct gccgcggcgt aactagttgc cagaagccag cgaggttagt tgcgtaagca
     6661 gtttcgctgg ttcatcacct gaaagctgtg cgtctacagg caaataccac caattttctt
     6721 ctgcaaaggc ccggcatttc accgcatctt tttcagtcat taccagcgtt tgcccggcgc
     6781 ttaccaacgc actgacatcc tcatggttca aagactgatg atcggccagc ggtacacatt
     6841 tttccggttg tacgccacac atcttcagcg tggcaaaaaa gcgcggcgga tgcccaatcc
     6901 ccgccatcgc cactacatgt tcaagctgag caacgtcaca acgcgtaccg gtacgtaaat
     6961 tcaccgcctg acccggcagc agatgcatgg ggatttcacc gctgcgaggg acaccgccgt
     7021 tgacgattac cgcatcaacc gactttaagc gccccgctcg ctcacgcatt ggccccgccg
     7081 gcaaccacca gccattgcca aagcgacgca caccatcaat aacgacaatt tccacatcac
     7141 gcgccagacg gtaatgctgt aaaccgtcgt cggttacgat gatctgcaca tcagggtgtt
     7201 gcgccagaat ggcttttacc gcatcagaac gaacgggaga aaccgcaaca ggcgcatcag
     7261 tgcgttgata aatcaacaca ggttcatcac ccgcctgtgc tgtggtggta tctgccgaca
     7321 ataacagcgg ataagattca gccttaccac catatccccg cgatacgacc cccacgcgaa
     7381 taccgcgctg ttgcaactgt tccaccagcc agacaacgac cggggttttt ccgttgccgc
     7441 ctgcggtgag attaccaacc acgacaaccg gtacgggggc acgccaggcg cgcttcagtt
     7501 ttagtttata gcaaagacgg atcgcgccac tcaccaggcc atacaaccag gagagtggca
     7561 gcaatagccg ccacaaaggg gattcaccag accagatttt ttcgatcatt ggccaaactg
     7621 cattttgtga agttgcgcgt aaacgccgcg gtgctcaagc aaatcgttat gcgtaccgcg
     7681 ttccacaatg acaccatcct cgacgaccac gatttcgtct gccttttcaa tggtagacaa
     7741 gcggtgggca atcaccagag aggtacggtt tttctgcaac tcatccagtg ccgcctgaat
     7801 cgcacgttcg gattcggtat ccagagccga ggtagcttca tccagaatca gaatcgggct
     7861 atcacgcagc aaggctcgag cgatagcaat acgctgacgc tgaccgccag agagcagcac
     7921 gccgttttca ccaatcactg tatcgagacc gttatccatc ttattgatga agtccatggc
     7981 gtaggccatc cgcgccgctt cttcaatttg ctcacggctg tactgttcag tccgtgcgta
     8041 agcaatgttg ttagcaaccg tatcgttaaa cagatggaca ttctgcgaca ccagagcaac
     8101 ctggttacgt aacgacgcca gggtatactc gcgcagatcg tgaccatcca tcaggatttc
     8161 gccttcatca atatcgtaaa aacgcgtgat caggctggcg atggttgatt tacctgaacc
     8221 agagcgtcca accagagcaa ccgtcttccc tgccggaatt ttcaggttga tgttacgcaa
     8281 tgcgggtacg tcacgtcccg gataagtaaa ggtgacattg cggaattcca cgtcgccagt
     8341 cgcacgctcg atcacgcgct taccttcgtc tttctcctgc tcactgtcca gaatggtaaa
     8401 cagcgtctga caagccgcca taccgcgctg gaactgggcg ttaacgttgg tcagcgattt
     8461 cagcggacgc atcagtgcaa tcattgaaga gaaaacaacg gtaatcgtac cggcagtcag
     8521 gttatccata acacttggga agctcgccgc atacagaaca aacgccagcg ccaaagaggc
     8581 gatcagctga atgatcggat cagagatgga agaggctgaa accattttca tcccctgaag
     8641 acgcattcgg ttactgactt tatcgaagcg tttcgtttcc acttcctgac caccgaaaat
     8701 caatacttct ttgtggccct tcagcatttg ttctgcactg gtggtcactt gccccatggt
     8761 gttctgcatg tttttactga tgttgcgaaa acgcttcgat actacgcgaa tcgcaatcga
     8821 aacaatcggt gccagcacaa tcaaaatgat cgacagttgc caactgtaat agaacatcat
     8881 gatgaacagg ccgatgatcg acgcaccttc acgcacaaca gtaatcagtg cgccggaaga
     8941 agaagaagca acctgttcgg aatcgtaggt gatacgtgac aacagcgtac ccgttgactg
     9001 tttgtcaaag aatgaaactg gcattcccat catgtgacca aacaggcggc gacgcatggt
     9061 cattaccacc tttcctgata cccaggagat acagtagctg gagacatagc tggtgatacc
     9121 acgtaaaatc atcagtccga tcaccaccag cggcatccac accagcacgg agcgatctgt
     9181 tttaccaaag ccatcatcaa gaagtggctt aaggagcgat aacatgaagg tatcgctggc
     9241 tgcgttgagg attaacgcta cgcccgccac gatcagaccc gctttgaaag gcgcaatggt
     9301 tggccacagt cggcggaatg tctgccacgt agagagatct ttgtcgttat gcattcaaaa
     9361 aaccagcatt tgttgaaata gccgcatatt ctacccgtta tccactggca cgccaaacca
     9421 ctgatgatac caacgaggta aaatttgctc tctgaggctg ctaatccgcc agccttgtgc
     9481 tgaaaaattg accgtcactt gcccctgatg tggagtatca agccattgat atcctttctg
     9541 ttgatagcga tgcttaactt tgttagaggg caatcgccat gcgttatagc gcgatgccga
     9601 tgcgagtgcc acttttccat tcactcgctg aattaatggc aacgatgatg aggtattact
     9661 gccatggtga ggtacctgaa gcaatgttgc ctgcacttgc tgccagtaac ggcttagcat
     9721 cttttgttca gctggggctt caatatcacc ggttagaaga atgctattcg tcccgtcatc
     9781 aaccttaacc acacaggaat ggttatttcc cttatcgttg ctaccttgta aaggccagtg
     9841 cgcgctgaaa cgcaatcctt gccactgcca cgcttcgcca cgcacacagg gctgatgatg
     9901 ttcccagttt aacggacttc tgatccataa catcggccat atatgcaata ttgaatccag
     9961 ccctccccgg tgatccagat gttcatggct cagaataacg ccttccggtt caagattatg
    10021 ccagtggagc caggggataa tcagttgttg cccactatcc ccttcaggcc agtcctgtgt
    10081 catagagaat cgctttgccg tttctggcta tcaccattgc cagcccttgc ccgacatcaa
    10141 gcatgtacag ctgccactcg tcaggtcgag gtttttgcca cagcggccag cacatcagca
    10201 agcctccagc cacacacatt gctggcaacg ttcgccaggc gtttaatcgc cataccacca
    10261 gtaagaacca tggggaaaat gatagccatt gccaacattc aacaatgttg atccaccctt
    10321 ccggcaaact ctttaacccc cagaaaagta aagccaaaga ccggtcggca agaaaccata
    10381 acccctgttc caggattaac ggcccgctaa aatgcacaac catcgcggcg aggatcaacg
    10441 gaaccgtgat aaatgtcacc aagggaattg caaatagatt tgcaataaac gaggtcagac
    10501 taatgccatg aaatatgacg atttgcatgg gcataagcag gagtgtgatt cccagttgca
    10561 gatggatgag ggaaacaact gcacgcaata ccggcggcag ttgccactca ggacagggaa
    10621 accactgata ccaaaaaatc agtgccgcga ccgcagcggc agagagccat aaactttgcg
    10681 agagaatggc aacaggatcc atcagcaaaa ttgccgccag acaacatatc catacatccc
    10741 agccactcca ctgtcgccca cttaacttaa gcattcccca cgtagcaagc gccaccacgg
    10801 tacgcaatgc aggaggttgc attcccgtca accaggcata aaaagcagca cagcagattc
    10861 cgccaattaa tggtatttgc cagtggatcc agcgcccagg cagaaaaatt tgtccactgc
    10921 gaatgagtcc ggcagccagc aacgccgcaa aagcgatgtg caatcccgat atcgccatta
    10981 aatgcgccgt tccagtatcg cgcatgatat ttttgatttc tttggggacg gataaccgtt
    11041 cccccatacc taaaccaaga ataaccgcat tccacggata gggttgcagc gttgtttgta
    11101 gtgacgccag atactgtgca cgcaggctac aattcggttc aatgacactt gcctgcagaa
    11161 aacggccggt gagcggctga tgctgggcaa tggcataacg ctggctatca aagccgccat
    11221 cattaagttg gccatgaact gcacgaactt tgagtttcat tgaccattgt tgtccagcac
    11281 aaaccgcttg cggaagttat tcgccataca gcacaaggcc tggcgcaggg aatatacgtc
    11341 gaccttgtag ataagtaatt tgaccgtaat gagtggtcat gccgtcagtt gcagtgatct
    11401 caacaattgc atcctgcgtc gcgccagtta aggtttctcc tgcccacaaa atttgctttg
    11461 ctgataatat gccccaaacg aaaaacagca acgtcagcgc gatataacgg acggttttaa
    11521 cagggataaa tgccagtacg caggcgaaga gagtcagaaa cgcaagggtt aatgtcctag
    11581 gcaattgggg caaaatcagc aacggaaaaa ttccgcaaat tatgcatacg ccgactgtcg
    11641 ttattttcat gcacccctcc ctgtttatgg aaagtgtgca actttgttga gcggttgtct
    11701 ggtgggaatt ttgcgttccc cgaggcacat tcagaagaat ttaacgaaag ggtgaaaact
    11761 gctcaaaact ctgcgaggca gcttccagtt cctgattaga gaattgaaaa aagcacccga
    11821 caggtgcttt tctcgcgttc aagtttgagt aaaaacttaa ccgtaaatat tggcgcgatc
    11881 gcgcagttct ttaccaggtt taaagtgagg aacgtatttt ccttccagtt ctactttatc
    11941 gccagtcttc ggattacgtc cggtacgtgg tgcgcggtag tgcaaagaga aactgccgaa
    12001 accgcggatt tcaatacgct cgccctgcgc aagagtcgag gccatatgct ccagcatctc
    12061 ttttactgca tcttcaaccg tcttggcggg aatgtgcgat tgctgggtgg caagtctttc
    12121 tatcaattct gacttggtca tgattcctcc ggttccttaa aggcaaatta agcggctgct
    12181 gttgctgcat tgattaaggg cggccgtagc cgcccttagt gcttgattac aggacgaaac
    12241 ctgcaatctg tcaagtaaac tcaacaaact tcggaataaa aatcccgaag agtcagagaa
    12301 ttactcgcct ttagctgctt tgaaagcttc agccattgcg ttgttggaga agtttgcatc
    12361 ttcctgtttg ttaacagttg cgattgcatc tttctcgtca gcttcgtctt tcgcacgaac
    12421 agacaggctg attgcgcggt ttttacgatc aacgccggtg aatttagctt caacttcgtc
    12481 gccaacgctc agaaccaggg tagcgtcttc aacgcggtca cgggatgctt cagaagcacg
    12541 caggtaacct tcaacgccgt cagccagttc tacggctgcg cctttagcgt caactgcagt
    12601 tactttaccg gttacgatag cgcctttctt gttcagagca acccagttgt tgaacggatc
    12661 ttctgcgagc tgtttaacgc ccagggagat acgttcacgt tctgcgtcaa cctgcagaac
    12721 aactgcagcg atttcgtcgc cttttttgta ttcacgaact gcttcttcgc ctgcaacgtt
    12781 ccaggagatg tcagacaggt gaaccaggcc gtcgatgccg ccgtccaggc cgatgaagat
    12841 accgaagtca gtgatagact tgattttacc ttcaacacgg tcgcccttgt tgtgggtttc
    12901 cgcgaactgc tgccacgggt tagctttgca ctgtttcaga cccagggaga tacgacgacg
    12961 ttcttcgtcg atatccagaa ccataacttc cactacatcg ccaacgttaa caactttgga
    13021 cgggtggatg tttttgttgg tccaatccat ttcggaaacg tgtaccagac cttcaacgcc
    13081 ttcttcgatt tcaacgaagc agccgtagtc agtcaggttg gtcacgcgac cagtcagttt
    13141 ggtaccttcc ggataacgtt tagcgatagc tacccacgga tcttcgccca gctgtttcag
    13201 gcccagggat acacgggtac gttcgcggtc gaacttcagc actttaacag tgatttcgtc
    13261 gcccacgttg acgatttcgc tcggatgctt aacgcgtttc caggccatgt cagtgatgtg
    13321 cagcaggccg tcaacgccgc ccagatcaac gaatgcaccg tagtcagtga ggttcttaac
    13381 gataccttta acttccatgc cttcctgcag gttttccagc agctgatcgc gctctgcgct
    13441 gttttcggat tcgataacgg cacgacgaga aacaacaacg ttgttgcgct tctgatccag
    13501 cttgattact ttaaattcaa gctctttgcc ttccaggtgc agagtgtcac gcaccggacg
    13561 aacgtctacc agagaacctg gcaggaacgc acgaataccg ttcagctcaa cagtgaagcc
    13621 gcccttaact ttgccgttga taacaccggt aacagtttca gcatcttcgt aagctttttc
    13681 cagcgtgatc caggcttcgt gacgtttagc tttctcacgg gacagcagag tttcaccgaa
    13741 gccgtcttct actgcgtcca gagcaacgtc aacttcgtca cctacctgga tttccagctc
    13801 gccctgggcg tttttgaact gctcagccgg gatggcggac tcagatttca gaccagcgtc
    13861 aaccagtact acgtctttgt cgatagcaac aacaacgcca cgaacgatag aacccgggcg
    13921 ggtttcgatt tcttttaagg actcttcaaa gagttgagca aaagattcag tcatgtttaa
    13981 tcttcaggtt tatatttaac gtccacctgg ctccatgccg gatggggttg tttaacatac
    14041 ccgctgtcat tccattgcaa cgggggtact gcaaattcgg tcgcttatgc gagagccaat
    14101 ttctggcgcg cgtattgtag cgctttttca atcaattgct caatgcttaa ggtggtggaa
    14161 tccaacacta aagcatcggc tgccggaacc agtggcgcta ccgctcggtt acgatcgcgg
    14221 tcgtcgcgtt ctttgatctc ggccaaaagg cgctcgaagt taacactaaa gcccttctcc
    14281 tgcaactgta gcatgcggcg atgcgcacgt tcttccgagg aggcgtcaag gaaaattttc
    14341 actggtgcat cagggaatac caccgttccc atgtcgcggc catcggcaat cagacctggt
    14401 aattcgcgaa acgcgcgttg gcgacgcaat aatgcttcac gaacgcgtgg gaatgccgcg
    14461 acttgtgaag ccgcattcgc cacttcctga gtacgaattt cgccgctgac atcttcccct
    14521 tcgaggatca cttccagatt gccattggtc gacacaaaac gtacatccag atgggatgcc
    14581 agcggtacca gcgcatcttc cgacgcaaca tcaacatggt gatgtaatgc cgccaatgcc
    14641 agtacgcgat aaattgcacc cgagtccagc agatgccatt gcaacgcttc cgccatagcc
    14701 ttacacaagg tgcctttccc tgcaccgctt gggccatcaa tggtaataac cggggcaatt
    14761 gccgtcatct ttatctcctt aaaacaggcg taccgttaac ataaccgcgc gcattatacg
    14821 cgccgacgcc acaaatcgtt aacgttgtgt gcaaaaacca gttttgtctg tattcagatg
    14881 gggatttatt gcgcaattat agcgggctgg cagaagacca gcccgaaaga tgacaatgat
    14941 ttacttacca gaggcgatac gatcacggat gtgttgcgca cgctctgttg atggtgggtg
    15001 agagtcaaac atggactggg tgcgaccgcc atccaggcta gccagttttt caaagctgcc
    15061 aaccagcccc tgggtgctga tgccacgttt cttcaacaga tcaaaagaga aatcatctgc
    15121 ttcggactct ttatcacggg agtatttagc attaatagcg ccttctgcga tatcacccaa
    15181 ttgtgagcgg gaaagctggg aagccacacc gctggtagct gaaatggcat cgcgtgcggc
    15241 aacgatcgca taagaagctt tcatttcagc cagcgagtga cccaacgcga cgtggcccag
    15301 ttcatggccc agaacacctt caatttcgtt gtcattcatc atgtccatca ggccactgta
    15361 gacacgaaca cagccgttcg ccatcgccca tgcgttgacg tcgctggtca tataaacctt
    15421 atagttgacc ggcgtgccat taatattgtt acccaatgct ttggcgattt ttgccagacg
    15481 tttaccgtac ttgctggatt tacttgcgac ttgattgccg ctgtccattt cggcacagcc
    15541 ctgattggca atcgcttttg catcggcatc gcttaatgtt gctgctttat aggcatttaa
    15601 accggagctg atagccatat tggtatcaat accgtgggta ttttgacacc ctgtcagtaa
    15661 tgctgcagag gtcgcaatcg ccagcagtaa tttagtattc ttcatgtgaa tcatccgttg
    15721 attaatcata tataaatatt atgtatcaac aacttacatt gacaagcgac gctaccatta
    15781 atcaaaatga ctcacaaggt ccgaaaaaaa acgccgacaa t
//
LOCUS       DAJTSI010000094        15410 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000094 DAJTSI010000000
VERSION     DAJTSI010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.094"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1175)
                     /locus_tag="OO327_002681"
     CDS             complement(<1..1175)
                     /locus_tag="OO327_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6019166.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRL"
     gene            complement(1374..1458)
                     /locus_tag="OO327_002682"
     tRNA            complement(1374..1458)
                     /locus_tag="OO327_002682"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1422..1424),aa:Leu,seq:caa)
     gene            1654..2673
                     /gene="ahr"
                     /locus_tag="OO327_002683"
     CDS             1654..2673
                     /gene="ahr"
                     /locus_tag="OO327_002683"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR6019167.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2802..4304
                     /locus_tag="OO327_002684"
     CDS             2802..4304
                     /locus_tag="OO327_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR6019168.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4423..5505)
                     /gene="lptG"
                     /locus_tag="OO327_002685"
     CDS             complement(4423..5505)
                     /gene="lptG"
                     /locus_tag="OO327_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR6019169.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5505..6605)
                     /gene="lptF"
                     /locus_tag="OO327_002686"
     CDS             complement(5505..6605)
                     /gene="lptF"
                     /locus_tag="OO327_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR6019170.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6872..8383
                     /gene="pepA"
                     /locus_tag="OO327_002687"
     CDS             6872..8383
                     /gene="pepA"
                     /locus_tag="OO327_002687"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR6019171.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMTNHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8517..8960
                     /gene="holC"
                     /locus_tag="OO327_002688"
     CDS             8517..8960
                     /gene="holC"
                     /locus_tag="OO327_002688"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR6019172.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8960..11815
                     /gene="valS"
                     /locus_tag="OO327_002689"
     CDS             8960..11815
                     /gene="valS"
                     /locus_tag="OO327_002689"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR6019173.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11869..13065)
                     /locus_tag="OO327_002690"
     CDS             complement(11869..13065)
                     /locus_tag="OO327_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCR6019174.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13258..13761
                     /locus_tag="OO327_002691"
     CDS             13258..13761
                     /locus_tag="OO327_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6019175.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13807..14223)
                     /gene="rraB"
                     /locus_tag="OO327_002692"
     CDS             complement(13807..14223)
                     /gene="rraB"
                     /locus_tag="OO327_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR6019176.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14385..15398
                     /gene="argF"
                     /locus_tag="OO327_002693"
     CDS             14385..15398
                     /gene="argF"
                     /locus_tag="OO327_002693"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR6019177.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3977 a         3932 c         3968 g         3533 t
ORIGIN      
        1 atcaacctac gctccccaag atgttccgcc ttatggatgt aagctgctcg cacagagcta
       61 cgctcctggt gactcatctg ccgctctacc gcatccctcg accataatcc cgactcaatc
      121 aacgaactac aagccatcgt cctgaagcca tgcccacaaa cctcgacctt cgtgtcatac
      181 cccattacgc gcaaagcctt gttcaccgta ttctcactca tcggcttacg tggatcgtga
      241 tcgcccacaa aaatcagctc tcgatttccg ctcatgcttt tgatcttttc caaaatactt
      301 agggcctgcc gcgacaaagg tacaaggtgt ggtgtccgca tcttcgagcc acgctgagaa
      361 tgcttgactc cttccagttg ctcacgctca cctgggattg tccacatagc cgtttcaaaa
      421 tctatttctg accagcgggc aaaacgcagc tcgctcgaac ggatgaaaac caataaagtg
      481 agttcaaccg ccagtcgagt taacggtctg ccagaatagt gatctatgcg atgaagtaat
      541 tcaggtatac ggttcagttc aagagctgca cggtgctgtc ttttcgccgt agcaaccgca
      601 cctgcaatct cttgcgcggg gttgtagtcg attaagccgc tctgaacggc aaagcgcata
      661 atcgcggtag tacgctgctg taaacgggcg gcaacttcga gccgcccgga tgactcgact
      721 gctttaatgg gtacaagaag atcccgcgtc ttcagttccg caatgttccg cttaccgata
      781 gcagtaaaga gattatcctc cagacttttc aaaacacgag cgctatgcga tgccgaccac
      841 ttctgattgc tggcgtgcca gtctctggcg actacttcaa acgttatcgc ctcttgttcc
      901 tgttctacct taacagcttt cttgttttca ctgggatcga caccattagc taaaagctta
      961 cgagcctcat cccggcgtgc cctggcatcc gccaacgaca cttcggggta tttccccaat
     1021 gccagcatct tctctttgcc gccaaagcga taacgtagcc gccagtattt ggagccgtta
     1081 gggtgaacca gcaaaaccat gccttcaccg tcggtcagct tataggcttt tgcttcaggc
     1141 ttagccgaac gaaccttcac atcactcaga gccatgatga gtatcctttc aagggttctg
     1201 tgtgggtaca aacattatcg aaccgggaaa tacccgcagt tgtacccgca tcagtaagtt
     1261 gatgtagatt gaatcaggtt gacttaggtt gagtgaaaaa gcgaggaaag ccttgcggat
     1321 actggatttt aggcacaaaa aaagacgtcc gttgacgtct attgatgttc cgatggtgcc
     1381 gaaggccgga ctcgaaccgg cacgtatttc tacggttgat tttgaatcaa ctgcgtctac
     1441 cgatttcgcc acttcggcac tgaagaggta tgcggaaaac gttgtggatt atacctgtcg
     1501 cacgccacca tgcaagcgcc agcttgcacc cacctcgcta agtgctgaaa ttttcagcat
     1561 tacactcagc gatatgtaac ctttgtcaca ctccaggcac cccgccctgc catgctctac
     1621 acttcccaaa caacaccaga gaaggcccca aaaatgtcga tgataaaaag ctatgccgca
     1681 aaagaagcgg gcggcgaact ggaagtttat gagtacgatc ccggtgagct gaagccacaa
     1741 gatgttgaag tgcaggtgga ttactgcggg atttgtcatt ccgatctgtc gatgatcgat
     1801 aacgaatggg gattttcaca atatccgctg gttgccgggc atgaggtgat tgggcgcgta
     1861 gtggcactcg ggagcgccgc gcaggataaa ggtttgcagg tcggtcagcg tgtcgggatt
     1921 ggctggacgg cgcgtagctg tggtcactgc gacgcctgta ttagcggtaa tcagatcaac
     1981 tgcgagcaag gtgcggtgcc gacaattatg aatcgcggtg gctttgccga gaagttgcgt
     2041 gcggactggc aatgggtgat tccactgcca gaaaatattg atatcgagtc cgccgggccg
     2101 ctgttgtgcg gcggtatcac ggtctttaaa ccactgttga tgcaccatat cactgctacc
     2161 agccgcgttg gggtaattgg tattggcggg ctggggcata tcgctataaa acttctgcac
     2221 gcaatgggat gcgaggtgac ggcctttagt tctaatccgg cgaaagagca ggaagtactg
     2281 gcgatgggtg ccgataaagt ggtgaatagc cgcgatccgc aggcactgaa agccctgtcg
     2341 gggcagtttg atctcattat caatactgtg aacgtcagcc tcgactggca gccttatttt
     2401 gaggcgctga cctatggcgg taatttccat acggtcggtg cggttctcac gccgctgtct
     2461 gttccggcct ttacgttaat tgcgggcgac cgcagcatct ctggttctgc taccggcacg
     2521 ccttatgagc tgcgaaagct gatgcgcttt gccgcccgca gcaaggttgc gccgacaacc
     2581 gaactgttcc cgatgtcgaa aattaacgac gccattcagc atgtacgcga tggtaaagcg
     2641 cgctaccgag tagtcctgaa agccgacttc tgatcccttt gccgggtgct gtcgcccggc
     2701 acttaaatct ctttggaagg cgtctcgcaa tctgctgaat ttagcggtga agagtgcaca
     2761 gccgttgcct atactccagg caggaaactg gaggaaatct catgagcgaa cccctgttaa
     2821 ttgcccgcac gccggacaca gaactgtttt tactgccggg aatggctaac cgtcacgggc
     2881 tgattactgg cgcaacgggg acgggtaaaa ccgttacgct gcaaaaactg gcagagtcat
     2941 tgtcggaaat cggcgtgccg gtgtttatgg ctgatgtgaa aggcgatctg accggtatcg
     3001 cgcaggcagg aacggcgtcg gaaaaactgc tcacaaggct taaaaatatc ggcgtcaatg
     3061 actggcaacc gcatgccaat ccggtggtgg tgtgggatat ctttggcgag aaaggccaca
     3121 cggtgcgggc gacggtttcg gatctggggc cgctgttgct ggcgcgactg ttgaatctca
     3181 acgatgtgca atctggcgtg ctgaatatta tcttccgcat tgcggacgat cagggattgt
     3241 tgctgctcga ctttaaagat ttgcgggcga ttacccagta catcggcgat aacgccaaat
     3301 ccttccagaa tcagtacgga aatatcagta gcgcatcggt tggtgccatc cagcgcggat
     3361 tactgtcgct ggaacagcaa ggcgcagcac acttctttgg tgagccgatg ctggatatca
     3421 aagactggat gcgcaccgat gccaacggta aaggcgttat caatatcctc agcgccgaga
     3481 aactttatca gatgccgaaa ctgtacgccg ccagcctgct gtggatgctt tcagagttgt
     3541 atgaacaatt gccggaagcg ggcgatctgg agaaaccaaa actggtgttt ttcttcgacg
     3601 aggcacatct gctgtttaac gatgcaccgc aggtactgct ggataagatt gagcaggtga
     3661 taaggcttat tcgctcaaaa ggcgtgggcg tctggttcgt ttcgcaaaac ccgtctgata
     3721 ttccggataa tgtgttcggc cagctcggta atcgtgttca gcacgctttg cgggctttta
     3781 cgccaaaaga tcagaaagca gtgaagacag cggcgcaaac catgcgggcc aatccggcat
     3841 ttgataccga aaaggcaatc caggaactgg ggaccggcga ggcgttaatc tcgtttctcg
     3901 atgcaaaagg aagtccttct gtggtggaac gggcgatggt gatcgcacct tgttcgcgaa
     3961 tggggccggt gacggaagat gagcgtaatg gcctgattaa tcactccccg gtgtatggca
     4021 aatatgaaga tgaggtggac cgcgagtccg cctatgagat gctgcaaaaa ggctttcagg
     4081 ccagtaccga gcagcaaaat aatccccccg tgaaagggaa agaggtggcg gtggatgacg
     4141 gtattcttgg tggattgaag gatattttgt ttggcactac cggaccacgc ggcgggaaga
     4201 aagatggtgt ggtgcaaaca atggcgaaaa gcgccgctcg ccaggtgacg aatcagattg
     4261 tacgcgggat gttggggagt ttgctggggg ggagaaaaag ataagtcaga cgtgatctgt
     4321 gcccctcacc ctaaccctct ccccaaaggg gcgaggggac cgttcgaact cgtatttgac
     4381 tttgtcaaca ataataaagg gagctttcgc tcccttgtta gattacgatt ttctcattaa
     4441 cagccacagg ctgattaaga agaagctggc acttggcaac agtgcgccga tgatcggcgg
     4501 gatgccgtaa accaacgtca gcgggccgaa gatctggtcc agtacgtaga agacaaaacc
     4561 gaagctgata cctgtgacca cacgcacgcc catcggtacg ctacgcagtg ggccaaagat
     4621 aaacgacagc gccatcagca tcatcaccgc cacggatagc ggctggaaga ttttgctcca
     4681 catgttgagc tgataacgtc cggcatcctg accgctcgac ttcagatact tcacgtagtt
     4741 gtgcaaacca ctgatggaga gtgcatccgg gtccagcgcc accacgccca gtttgtctgg
     4801 cgtgaggtcg gttttccagg tgccgctcac cgtctgcgaa ccggtaatct gtttcggatt
     4861 ggtcagatca gattcatcaa cctgcgacag acgccagact ttatgttccg ggtcaaactt
     4921 cgcggtagcg gcataacgta cggattgcag acgacgattc tcgttaaagg cataaatact
     4981 gatgccacct aacacctcgt cacctttaac ccgctcaatg tagacgaagt tgttgccatc
     5041 tttcgcccat aaaccttgct gggtagagag caacgagccg ccgtacatcg cctgcgcacg
     5101 gtagttacgc gccatctgtt cgccctgcgg cgcgacccat tcgccaatcg ccatcgtcag
     5161 caagaccagt ggaatggcgg ttttcatcac cgacagcgct acctgcatac gggtaaaacc
     5221 agaagcctgc atcaccacca gttcgctgcg ctgcgccagc atcccaagac caagcaacgc
     5281 cccaagaaga gccgccatcg ggaagaagat ctgcacatct ttcggcacgc tcagcaaggt
     5341 atacattcct gcgcctaacg cgtcgtaact cccctgcccg gcttttttca gctgatcgac
     5401 aaatttgata atgcccgaca gcgacaccag catgaacagc gtcatcatga tggtggtgaa
     5461 aatagtttta ccgatatagc ggtcaagtac gccaaaaggt tgcatcacac cgctccttta
     5521 cgcgaaaaac tggcgcgcag gcggcggacc ggcacggtgt cccaaaggtt gagaacaatc
     5581 gccaaagcca gataaatcag gttaacggtc cacatccaca gtgtcgggtc cagcttacct
     5641 ttaccgccgt tcgatttcag ggaggtctgg atcaggaaga aaagtagata cagcagcatg
     5701 gctggcagca tcgacagtac gcgtccctga cgcgggttaa ccacgctcaa cggtacaacc
     5761 ataagtgcca tcataaacac ggtgaatacc aacgtgatac gccagttcag ttctgcgcga
     5821 gcacgatcgg tgtcagtgtt ccacaatgtg cgcatgtcca tctggtcggt atcgttcggg
     5881 tcgagcgcca ccgcctggtg accaatgatc gcctgataat cctggaaatc cgtaatgcgg
     5941 aaatcacgta acagtgcagt gccttcgaag cgcgttccct ggttgagagt gacgacctgg
     6001 gagccgtcgc gcagctgggt taaatgtccg gaatcggcca ccaccacaga aggacgtgca
     6061 ttaccttttg gtcgaatttg tgcgaggaac acatctttga aatcgctgcc gtcaacgctt
     6121 tcgatgaaca gcaccgagct gccattagtc gcttgctgga attgcccttg cgccagcgcc
     6181 gccatgccag ggttcgcttt cgcttctgct aacacttcat cctgatgacg cgatgaccac
     6241 ggtcccgccc acatcacgtt aaccgccgcg acgattgccg tgaataccgc aaggatcatt
     6301 gccgctttca ccagaaccgc tttgctcaag ccgcaggcat gcattaccgt aatttcactt
     6361 tcggtataca gtttgcccag cgtcatcagc agcccgagga acaggcttaa tggcaggata
     6421 agctgcgcca tttccggcac gcccaacccg agaagggaga gcaccagatt cgccggaata
     6481 tcgccgtcaa ccgctgcgcc gaggatcctc actaactttt gacagaagaa gatcaaaagc
     6541 aagatgaaga gtatcgccag ctggcttttg agcgtctccc gcaccagata tcttatgatt
     6601 atcactttaa atacgcccgt aaaaactcgt cttttgcagg attttagctt gtttcatggc
     6661 ttaaacgtca tttattctct tgagtcgtcg aaatcgtcgc taagataatt atactcaacg
     6721 gattcacctc tcagattttg ttctgacgtg ccaatgccgt aataacgtta agattaacac
     6781 gaagtcatcg caacagcgga catgagttac gaaagcttgc aattctatct gtagccaccg
     6841 ccgttgtctt taagattcag gagcgtagtg catggagttt agtgtaaaaa gcggtagccc
     6901 ggagaaacag cggagtgcct gcatcgtcgt gggcgtcttc gaaccacgtc gcctttctcc
     6961 gattgcagaa cagctcgata aaatcagcga tgggtacatc agcgccctgc tacgccgggg
     7021 cgaactggaa ggaaaaccgg ggcagacatt gttgctgcac catgttccga atgtactttc
     7081 cgagcgaatt ctccttattg gttgcggcaa agaacgtgag ctggatgagc gtcagtacaa
     7141 gcaggttatt cagaaaacca ttaatacgct gaatgatact ggctcgatgg aagcggtctg
     7201 ctttctgact gaactgcacg ttaaaggccg taacaactac tggaaagtgc gtcaggctgt
     7261 cgagacagca aaagagacgc tctacagttt cgatcagttg aaaacgaata agagcgaacc
     7321 gcgtcgtccg ctgcgtaaaa tggtgttcaa cgtgccgacc cgccgtgaac tgaccagcgg
     7381 tgagcgcgcg atccagcacg gtcaggcgat tgccgccggg ataaaagcag cgaaagatct
     7441 cggtaatatg ccgccgaata tctgtaacgc cgcttatctc gcctctcagg cgcgccagct
     7501 ggctgacagc tacagcaaga atgtcatcac ccgcgttatc ggcgaacagc agatgaaaga
     7561 gctgggaatg cactcttatc tggcggtcgg tcagggttca caaaacgaat cgctgatgtc
     7621 ggtgattgag tacaaaggca acgcgtcgga agatgcacgc ccaatcgtgc tggtgggtaa
     7681 aggtttaacc ttcgactccg gcggtatctc catcaagcct tcagaaggca tggatgagat
     7741 gaagtacgat atgtgcggcg cggcggcagt ttacggcgtg atgcgtatgg tcgcggagct
     7801 gcaactgccg attaacgtta ttggcgtgct ggcaggctgt gaaaacatgc ctggcgggcg
     7861 tgcctatcgt ccgggcgatg tgttaaccac catgtccggt caaaccgttg aagtgctgaa
     7921 caccgatgcc gaaggccgcc tggtactgtg cgacgtgtta acttacgttg aacgttttga
     7981 gccggaagca gtgattgacg tggcgacgct gaccggtgcc tgcgtgatcg cgctgggtca
     8041 tcatattact ggtctgatga cgaaccataa tccgctggcc catgaactga ttgccgcgtc
     8101 tgaacaatcc ggtgaccgcg catggcgctt accgctgggt gacgagtatc aggaacagct
     8161 ggagtccaat tttgccgata tggcgaacat tggcggtcgt cctggtgggg cgattaccgc
     8221 aggttgcttc ctgtcacgct ttacccgtaa gtataactgg gcgcacctgg atattgcagg
     8281 aaccgcctgg cgttctggta aagcaaaagg cgcaaccggt cgtccggtag cgttgctggc
     8341 acagttcctg ttaaaccgcg ctgggtttaa cggcgaagag taagtttgcg ttttaccctc
     8401 accctaaccc tctccctaag ggagagggaa ccgatcgagc gcaatagaaa ttcattgctc
     8461 atctttcagg gtgaggaatg cagcaatatt taaatccacc acaagaagcc ccatatatga
     8521 aaaacgcaac gttctacctt ctggacaatg acaccaccgt cgatggctta agcgccgttg
     8581 agcaactggt gtgtgaaatt gccgcagaac gttggcgcag cggtaagcgc gtactcatcg
     8641 cctgtgaaga tgaaaagcag gcttaccggc tggatgaagc cctgtgggcg cgtccggcag
     8701 aaagctttgt tccgcataat ttagcgggag aaggaccgcg cggcagtgcg ccggtggaga
     8761 tcgcctggcc gcaaaaacgc agcagcagcc cgcgcgatat attgattagt ctgcgaacaa
     8821 gctttgcaga ttttgccacc gctttcacag aagtggtaga cttcgttcct tatgaagatt
     8881 ctctgaaaca actggcgcgc gaacgctata aagcctaccg cgtggctggt ttcaacctga
     8941 atacggcaac ctggaaataa tggaaaagac atacaaccca caagatatcg aacagccgct
     9001 ttacgagcac tgggaaaagc agggctactt taagcctaat ggcgatgaaa gccaggaaag
     9061 tttctgcatc atgatcccgc cgccgaacgt caccggcagt ttgcatatgg gtcacgcctt
     9121 ccagcaaacc atcatggata ccatgatccg ctatcagcgc atgcagggca aaaacaccct
     9181 gtggcaggtc ggtactgacc acgccgggat cgctacccag atggtcgttg agcgcaagat
     9241 tgccgcagaa gaaggtaaaa cccgtcacga ctacggccgc gaagctttca tcgacaaaat
     9301 ctgggaatgg aaagcggaat ctggcggcac cattacccgt cagatgcgcc gtctcggcaa
     9361 ctccgtcgac tgggagcgtg aacgcttcac catggacgaa ggcctgtcca atgcggtgaa
     9421 agaagttttc gttcgtctgt ataaagaaga cctgatttac cgtggcaagc gcctggtaaa
     9481 ctgggacccg aaactgcgta ccgctatttc tgacctggaa gtggaaaacc gcgaatcgaa
     9541 aggttcgatg tggcacatcc gctatccgct ggctgacggc gcgaaaaccg cagacggtaa
     9601 agattatctg gtggttgcga ccactcgccc ggaaaccctg ctgggcgata ccggtgtagc
     9661 tgttaacccg gaagatccgc gttacaaaga tctgattggc aaatatgtca ttctgccgct
     9721 ggttaaccgt cgtattccga tcgttggcga cgaacacgcc gacatggaaa aaggcaccgg
     9781 ctgcgtgaag atcacaccgg cgcacgactt taacgactat gaagtcggta aacgtcacgc
     9841 cctgccgatg atcaacatcc tgacctttga cggtgatatc cgtgaaagcg cccaggtgtt
     9901 cgataccaaa ggtaacgaat ctgacgttta ttccagcgag atcccggcag agttccagaa
     9961 actcgagcgt tttgctgcac gtaaagcagt cgttgctgcg gttgatgcac tcggcctgct
    10021 ggaagaaatt aaaccgcacg acctgaccgt tccttacggc gaccgtggcg gcgtggttat
    10081 cgaaccgatg ctgaccgacc agtggtacgt gcgtgccgat gtactggcga aaccggcagt
    10141 tgaagcggtt gagaacggcg acattcagtt cgtaccgaag cagtacgaaa acatgtactt
    10201 ctcctggatg cgcgatattc aggactggtg tatctctcgt cagctgtggt ggggtcaccg
    10261 tatcccggca tggtatgacg aagcgggtaa tgtttatgtt ggccgcaacg aagaagaagt
    10321 gcgcaaggaa aataacctcg gtgccaacgt tgccctgcgt caggacgaag acgttctcga
    10381 cacctggttc tcttctgcac tgtggacctt ctctaccctt ggctggccgg aaaataccga
    10441 cgccctgcgt cagttccacc caaccagcgt gatggtatct ggtttcgaca tcatcttctt
    10501 ctggattgcc cgcatgatca tgatgaccat gcacttcatc aaagatgaaa atggcaaacc
    10561 gcaggtgccg ttccacactg tttacatgac cggtctgatt cgtgacgacg aaggtcagaa
    10621 gatgtccaaa tccaagggta acgttattga cccgctggat atggttgacg gtatttcgct
    10681 gccagaactg ctggaaaaac gcaccggcaa tatgatgcag ccgcagctgg cggacaaaat
    10741 ccgtaagcgc accgagaaac agttcccgaa cggcattgag ccgcacggca ccgatgccct
    10801 gcgcttcacc ctggcggcgc tggcgtctac cggtcgtgac atcaactggg atatgaagcg
    10861 tctggaaggc taccgtaact tctgtaacaa gctgtggaac gccagccgct ttgtgctgat
    10921 gaacacagaa ggtcaggatt gcggcttcaa cggcggcgaa atgacgctat cgctggcgga
    10981 ccgctggatt ctggcggagt tcaaccagac tatcaaagcg taccgcgaag cgctggacag
    11041 cttccgcttc gatatcgccg caggcatttt gtatgagttc acctggaacc agttctgcga
    11101 ctggtatctg gagctgacca agccggtaat gaacggtgga acagaagccg aactgcgcgg
    11161 tactcgccat acgctggtga ctgtactgga aggtctgctg cgcctcgcgc atccgatcat
    11221 tccgttcatc accgaaacca tctggcagcg tgtgaaagta ctttgcggta tcactgccga
    11281 caccatcatg ctgcagccgt tcccgcagta tgatgcatct caggttgatg aagccgcact
    11341 ggccgacacc gagtggctga aacaggcgat cgttgctgta cgtaacatcc gtgcagaaat
    11401 gaacatcgcg ccgggcaaac cgctggagct gctgctgcgt ggttgcagcg cggatgcaga
    11461 acgtcgcgta aatgaaaacc gtggcttcct gcaaacgctg gcgcgtctgg aaagcatcac
    11521 cgtgctgcct gccgatgaca aaggtccggt ttccgttacc aagatcatcg acggcgcaga
    11581 gctgctgatc ccgatggctg gcctcatcaa caaagaagat gagctggcgc gcctggcgaa
    11641 agaagtggcg aagatcgaag gtgaaatcag ccgcatcgag aacaagctgg caaatgaagg
    11701 ctttgtcgcc cgcgcaccgg aagcggttat cgcgaaagag cgtgagaagc tggaaggtta
    11761 tgcagaagcg aaagccaagc tgattgaaca gcaggctgtt atcgccgcac tgtaatcaac
    11821 acagtgatga aaacgaaggc cggagcatgc tccggccttt tttatctctt acagaaatgg
    11881 taaagaaggc ataactcccc gagaaatact ggctaagaag cctttatctg gttgttcttc
    11941 tttattgatt aaaggaaggt catccaaatc gcccagcaaa tacgtatttt ttgcctgcca
    12001 gtcaatcatc catattttca gcagtgggta agccagacca cccgtaatcc cggatatcac
    12061 caccaacgca cacatgcgat aaagcatacc gtggtacgtt aaagttgagc gaaaacgaat
    12121 acgcccatca ttcagtgaca ggttgctcat aaaatgattt cgcaacgaca ccgttaaata
    12181 actggtgcta acagcaatcc caaaataata aattaactgc gcgattatca ttttacgttg
    12241 catttccata aattgctgta aattctcaat atcatcattt gcatacccag aactatcata
    12301 ctcatttaat atttgatcga agataatata accagcaaca gcgagaaaag gtaataaagc
    12361 taaaaatgcg aggatggcat attttataca atacgcagta tctaatttca ccttgaaacg
    12421 atgtataccg aaactggtat tgctccagag aaaactcatt accaggctat ataaagtacc
    12481 attaaaaata ccaatggaaa caataccaac tattgccatc agaaccacag atataataac
    12541 actacttgaa ctattggcat gtagcatctt tgtcgagata aagaaaacag tccccatccc
    12601 aatggccatt aaaatcggca agaaaaaggt tacccaccag aaccctttca aagagcaatt
    12661 aaaactaaat cttacgccgt tgagactggt cattaaggcc tgataacgca gtccttttgc
    12721 tgccataaat ataagcaaaa ccaacagtaa caaagtcaaa acacagccaa caagcggcat
    12781 atctgctgac actgtcatta agattgcgaa atagaaaaaa acaaaaacaa gacaactaac
    12841 aaaaacattc ccaccggtaa ttccataaga aaatcgttgt ccgttaactt ccatattagc
    12901 ataaagataa cgcttacatt tcattaatgc ccatggtaaa tagataccta gcgttataat
    12961 cgttaacaac acattcacca cacaaataag aaaatatctc tcacctgtac catgaaaaac
    13021 aaaagaatgg gacggaacat ccatttcatt aataacttga gccataaaaa tcccctgaat
    13081 taaaaaatcc attgatataa tttatagata cagacagtaa aatgttctgc cacgttacaa
    13141 tactcttatc agtaataggc tggcaaaaac tttattgctt tataagacgc ttgcgcacgt
    13201 aagaagagag tggtattaaa caaatgtata attactttat ttttattggg ttaaactatg
    13261 aataacattg cgccgcaatc acctgtaatg cgtcgcctga cgctgcagga taatcctgct
    13321 atcgccagcg tcatccgtca ggtttacgca gagtatggtc ttaccgccga taaaggctac
    13381 accgttgccg atccgaatct cgacgaactg tatcaagtgt acagccagcc tggccatgct
    13441 tattgggtgg ttgaatacaa tggcgaagtg gtcggcggcg gcgggattgc gccattaaca
    13501 ggtagtgaag cggacatttg cgaactgcag aaaatgtact ttcttcccgc tattcgcggt
    13561 aaagggctag cgaaaaaact ggccttaatg gcgatggagc aggcacgaga gatgggcttc
    13621 aaacgctgct atctggaaac gaccgctttt ttaaaggaag ccattgcgct ttatgagcat
    13681 ttgggctttg agcatatcga ctatgcgctt ggctgcacgg gccatgtcga ttgtgaagtg
    13741 cggatgctac gcgaactcta aaaaaatgcc cgctagtatg tgctatcggg cattttaatc
    13801 aattaattag tggcgaaccc cgtcatcgtc ttcatcgaca aaatcttcat cgtcgccatc
    13861 ttcgccgtta ggatcttcaa agtaagtgcc ccaaccgtcg tactcaacgt caaatttctc
    13921 cgccagcgtc atcagttgtt caacctgggc atcgatcaga tcggcattca acgcgcactc
    13981 gctgaggatg tcgcagcaaa tcacgatatc accatcttca acttccagct cttctggatc
    14041 ggtcacttcg taaccgagtt taaacgcttc aactgccgct ttttccagag tttccagatc
    14101 gtctgcggaa agatggtgtt cgatggtgta cagcgcgtcc ggatcgctgc catcttccag
    14161 taattcttca ataatcaaac gtgtttcttc acgctgttct tccagttgtt ccgggtttgc
    14221 catggctcat tcctcttaat gtcctgccga tactcttatt gtcacacacc actgacattg
    14281 cctccacctt tgtgacaaag atttatgctt tagacttgca aatgaataat aatacacata
    14341 aattgagttt taattcattg aggcgttagc cacaggaggg atctatgtcc gggttttatc
    14401 ataagcattt cctgaaatta ctcgatttca cgccagctga actcaacagc ctgctgcagt
    14461 tagccgcgaa gctgaaagct gataagaaaa gcggtaaaga agaagccaaa ctcactggta
    14521 aaaacatcgc gctcatcttc gaaaaagact cgactcgtac ccgatgctct ttcgaagttg
    14581 ccgcatatga ccagggggct cgcgttactt atcttggccc aagcggtagt cagattggtc
    14641 ataaagagtc gattaaagac actgcccgcg tgcttggtcg catgtatgac ggtattcagt
    14701 atcgcggcta tggtcaggag attgtcgaaa cactggcgga atacgctggc gtgccggtat
    14761 ggaacggcct gaccaatgag tttcacccca cgcagttgct ggcggatctc ctcaccatgc
    14821 aggagcattt acccggtaaa gcattcaacg aaatgacgct ggtctatgca ggtgacgcac
    14881 gtaacaacat gggcaattcg atgctcgaag ctgcggcgct taccggtctg gatttgcgtc
    14941 tggtcgcgcc acaggcatgc tggccagaag ctgcgctggt tacggaatgc cgcgccctgg
    15001 cacaacaaaa tggcgggaat attacgctga cggaagatgt agctaaagga gttgaaggtg
    15061 ctgactttat ctataccgat gtatgggtgt cgatgggtga agcaaaagaa aaatgggctg
    15121 aacgcattgc attactgcgt gattatcagg tgaacagcaa gatgatgcag ttgaccggta
    15181 acccggaggt caaattcctc cattgcctgc ccgcgtttca tgacgaccaa acgacgcttg
    15241 gcaaaaaaat ggcggaagag tttggcctac atggcggaat ggaagtgact gatgaggtct
    15301 tcgaatctgc cgccagcatt gtatttgatc aggcggaaaa ccgcatgcat accatcaaag
    15361 cggtgatggt cgcgacgctc agtaaattaa ataattaata tctctataaa
//
LOCUS       DAJTSI010000095        15392 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000095 DAJTSI010000000
VERSION     DAJTSI010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15392
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.095"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..623)
                     /locus_tag="OO327_002694"
     CDS             complement(<1..623)
                     /locus_tag="OO327_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6019178.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTL"
     gene            complement(763..1950)
                     /gene="metC"
                     /locus_tag="OO327_002695"
     CDS             complement(763..1950)
                     /gene="metC"
                     /locus_tag="OO327_002695"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR6019179.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            2202..2936
                     /gene="exbB"
                     /locus_tag="OO327_002696"
     CDS             2202..2936
                     /gene="exbB"
                     /locus_tag="OO327_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6019180.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            2943..3368
                     /gene="exbD"
                     /locus_tag="OO327_002697"
     CDS             2943..3368
                     /gene="exbD"
                     /locus_tag="OO327_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR6019181.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(3640..4524)
                     /gene="yghA"
                     /locus_tag="OO327_002698"
     CDS             complement(3640..4524)
                     /gene="yghA"
                     /locus_tag="OO327_002698"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR6019182.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            4715..5209
                     /locus_tag="OO327_002699"
     CDS             4715..5209
                     /locus_tag="OO327_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR6019183.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            complement(5249..6289)
                     /gene="gpr"
                     /locus_tag="OO327_002700"
     CDS             complement(5249..6289)
                     /gene="gpr"
                     /locus_tag="OO327_002700"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR6019184.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            6446..7333
                     /locus_tag="OO327_002701"
     CDS             6446..7333
                     /locus_tag="OO327_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6019185.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            7452..7739
                     /locus_tag="OO327_002702"
     CDS             7452..7739
                     /locus_tag="OO327_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR6019186.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            7928..9046
                     /gene="hybO"
                     /locus_tag="OO327_002703"
     CDS             7928..9046
                     /gene="hybO"
                     /locus_tag="OO327_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR6019187.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            9049..10035
                     /gene="hybA"
                     /locus_tag="OO327_002704"
     CDS             9049..10035
                     /gene="hybA"
                     /locus_tag="OO327_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR6019188.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            10025..11203
                     /gene="hybB"
                     /locus_tag="OO327_002705"
     CDS             10025..11203
                     /gene="hybB"
                     /locus_tag="OO327_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR6019189.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            11200..12903
                     /gene="hybC"
                     /locus_tag="OO327_002706"
     CDS             11200..12903
                     /gene="hybC"
                     /locus_tag="OO327_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR6019190.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            12903..13397
                     /locus_tag="OO327_002707"
     CDS             12903..13397
                     /locus_tag="OO327_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR6019191.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            13390..13878
                     /gene="hybE"
                     /locus_tag="OO327_002708"
     CDS             13390..13878
                     /gene="hybE"
                     /locus_tag="OO327_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR6019192.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            13871..14212
                     /gene="hypA"
                     /locus_tag="OO327_002709"
     CDS             13871..14212
                     /gene="hypA"
                     /locus_tag="OO327_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6019193.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            14346..14594
                     /gene="hybG"
                     /locus_tag="OO327_002710"
     CDS             14346..14594
                     /gene="hybG"
                     /locus_tag="OO327_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR6019194.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(14717..>15392)
                     /gene="yghU"
                     /locus_tag="OO327_002711"
     CDS             complement(14717..>15392)
                     /gene="yghU"
                     /locus_tag="OO327_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR6019195.1"
                     /translation="LLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIPALRDHT
                     HNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGGFGHFYH
                     YAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGNVVLGGV
                     YDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDASDFETNT
                     EDKRQG"
BASE COUNT         3609 a         3920 c         4115 g         3748 t
ORIGIN      
        1 agcgtgccta acaagccagc ggttaacaag gcaattggca ggatcatcag gaacgtcatt
       61 acctgatctt catggcgttt aacgaacgga atcatactta aggcatagcc aaaactggtt
      121 accacgctga cccacagcaa tccacttaac cagttgaaaa actggaagcg gcggtttggc
      181 agaccggaaa ttcccgccat ggttggcagc agcgtacgga caaatgcaag aaaacgtcca
      241 gccagcagcg ccagcagacc gtggcggtca aacatgcagg tggcgcgctg gtgatattta
      301 gcaggaagct gtgccagcca gcctttcacc gttttggtat tccctaacca gctcccctga
      361 atataactta gccagcagcc cagacttgct gcggcggtca gaatcgcaat cgtaggcaga
      421 aaatccataa ccccttgggc aatcaatgcg cctgccagta tcaacaagct gtcgcctggc
      481 aaaaatgagg cgggcagcag gccgttttct aaaaacagcg tggcaaacat gacaaagtaa
      541 acaacgctaa caatatgagg atccgccagc gcggcaaagt cgtgttgcca gagcgcagcg
      601 atgatatctt gaataacagc catggacttt cctgtggaac agcgtttaag cctttatctg
      661 cctattgtac tcctgaatcg tccgggacgc cttgatcccg gacgcaacaa tttaagactt
      721 ttcacaataa aatgtctgca aaattgtcca aaagtggcaa tgttatacaa tccgtgcaaa
      781 accggcgtcc agatcggcaa tcagatcgtc gacatcttcc agaccaatat gcaggcgaat
      841 caaggtcccg ctaaaatcga tctcgccttg tgggcgaatg gcggcgatat gttctggttg
      901 atttgccagg atcaacgatt catacccgcc ccacgagtag gccatgctga ataaactgaa
      961 gttatccaga tagttcgcca gctcttcatc attgagtttt ttcttaagca caaaggaaaa
     1021 tagcccgctg ctgcctgtaa agtctcgttt ccagaattcg tgtcctttac tgccaggcag
     1081 ggcagggtgg ttaactcgcg caacttgcgg atgttctgcc agccattcgg ccactttcag
     1141 actgctttca tgatgttgac gcaaacgcac acctaatgtg cgcaggccac ggctggttat
     1201 ataggcggta tcggcatcga ccatctggcc catcagatag gcattttccc gtagctgctc
     1261 ccagcaacgg gcattgcaca cggcagtgcc aatcatcgca tctgaatgcc caaccagata
     1321 tttggtggcg gcctgaatag aaacatcgat gccaaaatcc agcgccttaa acagcacacc
     1381 ggctgcccag gtgttgtcga tcataatgat ggcatccggc accacactgc gcacggcggc
     1441 aacaatcgcc ggaacgtcgt ggacttccat agtgatggaa cccggcgatt ccagaaacac
     1501 gattttagtg tttggctgca gatgcttaac gatatcggca ccaatcagcg gatcaaacca
     1561 cgatgtcgtt acgcccagtt tgctgaggat tttgctacag aaatcctgac tcggttcata
     1621 ggcggtgttg gtcatcaaca catgatcgcc ctgttcgaca aaagcaagaa tcgaattagc
     1681 aaccgctgcc gccccgcagg gaaatagcgc gcagcctgcg ccaccttcca gttcacacat
     1741 cgcttgttgt aaggaaaaat gggttaacgt tccgcgccgt ccatagaaca actcgccatt
     1801 ggcgcgattg cgcgtcgcgt gctttttggc ttccatactc tcaaatacca gcgaagaagc
     1861 gcgctgaatc acgctattta ccgcgccaag agtgtatttt ttgctgcgtc ctgcattcac
     1921 cagttgagta tcaagctttt tgtccgccat gtcgggattc ctgtttttat acgtctggat
     1981 gtctaaacta gcatgaatat tcgcgggcgc atcctgaaga attggcataa agcagaaaat
     2041 tttcgcaaat ggcgtgctgg tgactttttt actgcgtaaa cgcaaggaaa agaaagcaaa
     2101 ggtacggcag tatgcaaata gtaatgagaa cgactatcaa ttcgacgtcg ttttgatatt
     2161 attatgcgca gattttgtga cttgcgtcct ggagatacac agtgggtaat aatttaatgc
     2221 agacggacct ttccgtctgg ggtatgtatc agtacgccga tattgtcgtt aagtgcgtga
     2281 tgattgggct tattttggcc tctgtagtca cctgggcaat cttcttcagt aagagcgtag
     2341 agttcttcaa tcagaagcgt cgccttaagc gcgagcagca actgctggct gaagcacgtt
     2401 ccttaaacca ggccaacgat atcgccgctg attttggtag caaaagctta agcctgcatt
     2461 tgctcaatga agcgcagaat gagctggaac tgtcagaagg cagcgacgat aacgaaggta
     2521 ttaaagaacg taccagcttc cgtctggagc gtcgggtcgc cgcagtgggt cgtcaaatgg
     2581 gccgcggtaa cggctatctg gcaaccatcg gcgcgatttc tccgttcgtt ggtctgtttg
     2641 gtacggtctg gggcatcatg aacagcttta ttggtatcgc gcaaacgcag accactaacc
     2701 tggcagtcgt tgcgccgggt atcgcagaag ctctgttagc aacggcaatc ggcctcgtgg
     2761 cagcgattcc tgcggtcgtt atctataacg tatttgcacg ccagattggc ggctttaaag
     2821 cgatgctggg tgatgttgca gcgcaggtat tgttgctgca aagccgtgac ctagatctgg
     2881 aagccagcgc cgctgcgcat ccggttcgtg tcgcacaaaa attacgcgca ggataatgtc
     2941 cgatggcaat gcatcttaac gaaaacctcg acgataacgg cgaaatgcat gatatcaacg
     3001 tgacgccgtt tatcgacgtg atgttggttc tgctgattat ctttatggtg gcggcaccgt
     3061 tagcgacggt agatgtgaag gtgaacttgc ctgcttctac cagcacgccg cagccgcgcc
     3121 cggaaaaacc ggtttatctg tcggtgaagg cagacaactc gatgtttatc ggtaacgatc
     3181 cggtcaccga tgaaacaatg attacggcgt tgaatgcgtt aaccgaaggc aagaaagaca
     3241 ccaccatctt cttccgagcg gacaaaaccg tcgattacga gacgttgatg aaggtaatgg
     3301 atacgctgca tcaggcgggt tacctgaaga taggtctggt cggcgaagaa accgccaaag
     3361 cgaagtaaag tagaattgcc tgatgcgcta cgctcatcag gcctacaaaa tctattgcaa
     3421 catgttgaat cttcgtgcgt ttgtaggccg gataaggcgt tcacgacgca tccggcatta
     3481 ggtgctcaat gcctgatgcg ctacgcttat caggcctaca aaatctattg caacatgttg
     3541 aatcttcatg cgtttgtagg cggataaggc gttttcgcca catcaggtaa gagtgaattc
     3601 acaatgatgc ccggttgctt ttcacaaccg ggcatttttt taacctaaat gctcgccgcc
     3661 gcacacgccg tgcacttctg cggtgacgta gctcgactcc tgacttgcca gataaacata
     3721 tacaggggcc agttccgccg gttgccccgc acgcttcatc ggcgttttct gaccaaactg
     3781 cgggatctta tcctgcgttt gtccgccgga aatttgcagt gccgtccaga tagggcctgg
     3841 cgcgacaata ttcacccgaa tacctttctc cgcgacctgt tttgccaggc cacggctgta
     3901 gttcagaatc gccgccttcg tagccgcata gtccagtaaa tgcggacttg gctggtatgc
     3961 ctggattgac gaagtggtga tgacacttgc acctttcggt agcaggggga tcgcttcctg
     4021 ggttagccag aacagcgcga aaacgttaat ggcaaaggtc ttttgaaact gttcgctggt
     4081 gaggtctgca atatccggaa tggcaacctg tttcccggcg accagcgcca taatatccag
     4141 cccgcctaac gccttgtgcg cttcgtgaac cagcgaacgg gcgaatttct catcgcttaa
     4201 atcgcctggc agcagaacgg ctttgcgtcc gcattcttca atgatctttt tcacatcctg
     4261 agcgtcttct tcttccacgg gaagataact gatcgccacg tcagcccctt cacgcgcgta
     4321 agcgatggcg gcagcgcgac ctattccgga atcgccccct gtcaccagtg ctttacgatc
     4381 tttcaggcga ccgctaccaa cataggtttt ctcgccgcaa tccggtatcg gtgtcatctt
     4441 cgcctggatg cctggcgtcg gttgtttctg tttgggatat tcaccagtgt aatactgcgt
     4501 ggtcgggtct tttaaatgag acatcgtttt tctcccttca ggttcaacgt cctttaaggg
     4561 tagacgctct cgatgcgttg ataagggaac caggaagatc cctaaacctc agaattatgc
     4621 gacaaaggtt taacgtatat gttgatttgc tgttgcgtgc tgtttactca attgcgatat
     4681 actgttgccc gttttaacta cacgacagga atgtatggaa cgttttcttg aaaatgcaat
     4741 gtatgcttct cgctggctgc ttgcccccgt gtactttggc ctttcgctgg cgttagttgc
     4801 cctggcgctg aagttcttcc aggagattat tcacgtactg ccgaatatct tctcgatggc
     4861 ggaatcagat ttgatcctcg tgttgctgtc gctggtggat atgacgctgg ttggcggttt
     4921 gctggtgatg gtgatgtttt ccggttatga gaatttcgtc tcacagctgg atatctccga
     4981 gaacaaagag aagctgaagt ggctggggaa aatggacgca acgtcgctga aaaacaaagt
     5041 agcagcgtcg attgtggcaa tttcttccat tcacttactg cgcgtcttta tggatgcgaa
     5101 aaatgtcccg gataacaaac tgatgtggta cgtcattatc catctgacgt ttgtgctttc
     5161 tgcatttgtg atgggctatc ttgaccgact gactcgtcat aatcactgat ctgattcggg
     5221 cgcggttcgc gcgcccgtta ttaacaggtc atttatcgga cgacgcctgc cacagattca
     5281 gctcgccatc ggcgatatgc tgatcaatct gcgccagctc ctcggtgcta aatgtcagat
     5341 tattcagcgc ctgcacgttc tcctctagtt gctccgcgcg gctggcacca atcaataccg
     5401 acgtcacgcg atcatctttc agcaaccagc ttaacgccat ttgcgccatt gattgtccac
     5461 gctgctgtgc catttcattc aataagcgca ggctgttgag gttggcttcg gtaagcattt
     5521 tcggcgtcag accacgaact ttattccctt cacgatgcat ccgtgaatct tgcggaatgc
     5581 cgttgagata ttttccggtc agcaatccct gagccagagg agtaaaggca atacagccca
     5641 cgccgttatt ttgcagggta tccagcaggc cgcttttatc cacccagcgg ttcagtaaat
     5701 tgtacgaagg ttgatgaatt aaaagcggaa ttttccactc gtgcagcaac tcgaccattt
     5761 tttgcgtccg ctctggcgag taagaggaga tcccgacata cagcgcctta ccgctttgta
     5821 ccgcatgagc cagcgcagag gcggtttctt ccatcggcgt attttcatcg acgcgatgag
     5881 agtaaaagat atcgacatac tcaagcccca tacgcttcag gctttggtcg aggctggcga
     5941 gcaggtattt acgtgaaccg ccagagccgt acgggccggg ccacatatcg tagccagcct
     6001 tggtagagat aatcagttca tcgcgataag cagcaaaatc ctcgcgcagc aggcgaccaa
     6061 agttctcttc tgcgcttcct ggaggcggcc cgtaattgtt ggctaaatca aagtgcgtaa
     6121 tgcctaaatc aaacgcttta cgcaggattg cacgctgtga ttccagcgcg ttaacgtgac
     6181 cgaaattgtg ccataaaccg agcgataatg cgggcaggcg taaaccactt tttccgcaat
     6241 agcggtactg catctgcccg taacgttcgg gattcgctaa ccagaccatg acctctcctt
     6301 tccaccgttc aatttcgaaa caatgtttct agtttagcga ttcgccagcg cgtatcccgt
     6361 agtctggctc acagagtgac gaaaaactgg gcaaaaacac gcgcttatgc tttgcttaaa
     6421 aaaacaccag ttgaggaatg caacgatgcc gcgtttaacc gccaaagatt tcccacaaga
     6481 gttgttggat tactacgact attacgttca cgggaaaatt tcgaaacgtg agttcctcaa
     6541 ccttgcggcg aagtatgcgg tgggcgggat gacggcatta gcgttgtttg atttgctcaa
     6601 gccaaattat gcgctggcga ctcaggtaga gtttaccgac ccggagattg ttgctgagta
     6661 catcacgtat ccttcgccaa atggtcacgg cgaggtacgg ggttatctgg tgaaacccgc
     6721 aaaaatgagc ggcaaaacgc cagccgtggg ggtggtgcat gagaatcgtg gactgaatcc
     6781 gtatatcgaa gatgtggcac ggcgagtggc gaaggcgggg tatatcgccc tggcacctga
     6841 cggattaagt tccgttggag gttatccggg aaatgatgat aaaggtcgtg agctgcaaca
     6901 gcaggttgat ccaaccaaac tgatgaatga tttctttgcc gcaattgagt ttatgcaacg
     6961 ctatccgcaa gcgacaggta aagtgggtat taccggattt tgctatggcg gtggcgtatc
     7021 gaacgcggcg gctgtcgcgt atccggaact ggcctgcgcg gtgccgtttt atggtcgtca
     7081 ggcacccact gccgatgtgg cgaagattga agcgccttta ctactccact tcgcggaact
     7141 ggacacccga atcaacgagg gctggcctcc ttacgaggcg gcgttgaaag acaataataa
     7201 ggtttatgag gcttatatct atccgggggt taatcacgga ttccataatg attccacgcc
     7261 ccgttatgac aaatctgccg ccgatcttgc ctggcaaagg acactgaaat ggttcgataa
     7321 atatctctcc tgataggttt atctcttacg ggattacgtc ttaaacaagt atgaaaaaat
     7381 agcgtgcgca aaagtcgttc tttgcctaaa atatcgttat atatggcgat atataatgaa
     7441 tgaggtgaac gatgaataac cattttggta aaggcttaat ggcgggatta aaagcaacgc
     7501 atgccgacag tgcggttaat gtgacaaaat tctgtgccga ttataaacgc ggttttgtat
     7561 taggctactc acacaggatg tacgaaaaga ccggagatcg ccagcttagc gcctgggaag
     7621 cgggtattct gacgcgccgc tatggactgg ataaagagat ggtaatggat ttctttcgtg
     7681 agaataattc ctgttctacg ttgcgctttt ttatggccgg ttatcgcctc gaaaattgat
     7741 caaacatacg tattatcttg ctttaattaa ttacactaat gcttcttccc ttcgttttag
     7801 cgccccgccg cagtatcatg atatcgataa ccataataaa tgtgtggtaa atggcgcatc
     7861 gatcgcatta ttgattttgc gattgaggca aaatatatgc caggttctcg caacggaata
     7921 actataaatg actggagata acaccctcat ccattctcac ggcattaacc gtcgtgattt
     7981 catgaagctt tgtgcagcat tagccgccac catggggtta agtagcaaag ccgctgcaga
     8041 gatggccgaa tcggttacta acccgcagcg tccgccagtt atctggattg gcgcgcagga
     8101 gtgcaccggt tgtacggaat ctctgcttcg tgcaacgcat ccaacggtag aaaacctcgt
     8161 gctggagact atctctctgg aatatcacga agtgctttcc gccgccttcg gtcatcaggt
     8221 cgaagagaac aaacataacg ctctcgagaa gtacaaaggg cagtatgtgt tagtggtgga
     8281 tggttccatc ccattaaaag ataacggtat ttattgcatg gttgccggtg agccgattgt
     8341 ggatcacatc cgcaaagcgg cggaaggctc agcagccatt atcgctattg gttcctgctc
     8401 cgcgtggggc ggtgttgccg cagctggagt taacccaact ggcgcagtca gcttgcaaga
     8461 agttctgcca ggcaaaaccg ttatcaatat tccgggctgc ccgccgaacc cgcacaactt
     8521 cctcgcgacc gttgcgcaca tcatcactta cggcaaaccg ccgaaactgg atgacaaaaa
     8581 ccgtccgacc ttcgcctatg gccgtctgat tcacgaacac tgcgaacgtc gcccgcactt
     8641 cgatgctggt cgttttgcca aagagttcgg tgatgaaggc caccgcgaag gctggtgctt
     8701 gtaccacctc ggctgtaaag ggccagaaac ttacggcaac tgctcaacgc tgcaattctg
     8761 cgatgttggc ggtgtgtggc cggtggcgat tggtcacccg tgctatggct gtaacgaaaa
     8821 aggtatcggc ttccataaag gcatccatca gcttgccaac gtcgaaaatc agactccgcg
     8881 ttcacagaaa ccggatgtta acgctaaaga gggcggcaac gtctctgcag gcgctattgg
     8941 tttgctcggc ggtgtggttg ggctggttgc cggtgtcagc gtgatggcgg tgcgtgaact
     9001 gggtcgtcag caaaagaaag ataacgctga ctcacgggga gaataaccgt gaacagacgt
     9061 aattttatta aagcagcctc ctgcggggca ttgctgacgg gcgcgctgcc gtctgtcagt
     9121 catgcggctg ctgaaaaccg cccgccaatt ccgggatcgc tggggatgtt gtacgactcg
     9181 accttgtgcg taggctgcca ggcttgcgtc accaagtgtc aggatatcaa cttccctgaa
     9241 cgtaacccgc aaggggaaca gacctggtcg aacaacgaca aactgtcgcc gtataccaat
     9301 aacatcattc aggtgtggac cagcggcaca ggggtcaaca aagaccagga ggagaacggc
     9361 tacgcgtaca ttaagaaaca gtgtatgcac tgcgtcgatc cgaactgtgt ctctgtgtgc
     9421 ccggtctctg cactgaaaaa agatccgaaa accggcattg tccattacga caaagacgtg
     9481 tgcaccggtt gccgttactg catggtcgcc tgtccgtaca acgtgccgaa gtacgactac
     9541 aacaacccgt ttggtgcgct gcataagtgc gagctgtgca accagaaagg tgtggaacgt
     9601 ctcgataaag gcggtctgcc tggctgcgta gaagtgtgcc cggcgggcgc ggtgattttc
     9661 ggtacgcgtg aagagctgat ggcggaggcg aaaaaacgtc tggcgctgaa gcctggcagc
     9721 gaataccact atccgcgtca gacgctgaaa tctggcgaca cttacctgca tacggtgccg
     9781 aaatattatc cgcatctgta cggcgagaaa gagggcggcg gtactcaggt tctggtactg
     9841 acgggtgtgc cttatgaaaa tctcgacctg ccgaaactgg acgatctttc taccggtgcg
     9901 cgttccgaaa atattcaaca caccctgtat aaaggcatga tgctaccact ggctgtgctg
     9961 gcgggcttaa ccgtgctggt tcgtcgcaac accaaaaacg accatcacga cggaggagac
    10021 gatcatgagt catgatccac aaccgctggg cggcaaaatc atcagtaaac cggtcatgat
    10081 ttttggaccg ttaatcgtca tctgtatgct cctgattgtg aagcgtctgg tgttcggtct
    10141 gggctctgtc tctgacctga acggcggctt cccgtggggc gtgtggatcg cgtttgacct
    10201 gctgattggc accggctttg cctgtggcgg ctgggcgctg gcgtgggcgg tatacgtctt
    10261 taaccgtggg caataccatc cgctggtgcg tccggcgctg ttggcgagtc tgtttggtta
    10321 ctcactgggt ggcttgtcga tcactatcga cgtgggtcgc tactggaacc tgccgtactt
    10381 ctacattccg ggtcacttca acgtgaactc ggtactgttc gagacggcgg tctgtatgac
    10441 catctacatc ggcgtgatgg ctctggagtt tgctccggca ctgtttgaac gtctgggctg
    10501 gaaggtgtcg ctacaacgtc tgaacaaggt gatgttcttc atcatcgcgc tcggtgcgct
    10561 gctgccgacc atgcaccagt cttcaatggg gtcgctgatg atctcggcgg gctacaaggt
    10621 gcatccgctc tggcagagct atgaaatgtt gccgctgttc tcgctgctga cggcgttcat
    10681 catgggcttc tcgattgtca tctttgaagg ttcactggtg caggcgggtc tgcgtggcaa
    10741 cggtccggat gaaaagagcc tgttcgtcaa gctgaccaac accatcagtg tgttgctggc
    10801 gattttcatc gggctgcgct ttggcgagct tatctatcgc gacaagctgt cgttagcgtt
    10861 tgccggtgac ttctactccg tgatgttctg gattgaagtc ctgctgatgc tcttcccgct
    10921 ggtcgttctg cgtgtggcga agctgcgtaa tgattcccgt atgctgttcc tgtcagcact
    10981 gagcgcactg ttaggttgtg caacctggcg tctgacctat tcgttggtgg cattcaaccc
    11041 gggcggcggt tacgcctact tcccgacctg ggaagaactg ttgatttcta ttggttttgt
    11101 ggctattgag atttgcgctt acatcgtact cattcgtcta ctgccgatac ttcctccttt
    11161 aaaacaaaac gatcataatc gtcatgaggc gagcaaagca tgagccagag aattactatt
    11221 gatccggtaa cccgtattga ggggcattta cgcatcgatt gcgaaatcga aaatggcgtc
    11281 gtttcgaaag catgggcttc cggtaccatg tggcgcggca tggaagagat cgtgaaaaac
    11341 cgcgatccgc gcgatgcatg gatgattgtg caacgtatct gtggcgtatg tactaccact
    11401 cacgcgctgt cttccgttcg tgcggcagaa agtgcgctga atatcgacgt tccggttaac
    11461 gcgcaataca tccgtaacat cattctggct gcgcacacca cgcatgacca tatcgttcat
    11521 ttctatcagc tttcggcgct ggactgggtg gacatcactt ctgcactgca agctgaccca
    11581 accaaagcct ccgaaatgct gaaaggcgtt tcgacctggc atctgaacag tccggaagag
    11641 ttcactaaag ttcagaacaa gatcaaagat ctggttgcca gcggtcagtt gggtattttc
    11701 gccaatggct actggggtca cccggcgatg aaactgccgc cggaagtgaa cctgattgcg
    11761 gtagcgcact acctgcaggc gctggagtgc cagcgtgacg ctaaccgcgt cgtggcgctg
    11821 ctgggcggta aaacgccgca cattcagaac ctggctgtgg gtggtgtcgc gaacccaatc
    11881 aacctcgacg ggttgggcgt gttgaacctt gagcgcctga tgtacatcaa gtctttcatc
    11941 gacaaactga gcgactttgt tgagcaggtt tataaggttg ataccgcggt tatcgccgcg
    12001 ttctacccgg aatggctgac gcgcggtaaa ggtgcggtga actacctgag cgtgccggaa
    12061 ttcccgaccg acagcaaaaa cggcagcttc ctgttcccgg gaggctacat tgagaatgcg
    12121 gatctgtcct cgtatcgtcc gatcacttct cattccgatg aatacctgat caaaggcatt
    12181 caggaaagcg cgaagcactc ctggtataaa gacgaagcgc cacaggcgcc gtgggaaggt
    12241 accaccattc cggcttatga tggttggtct gacgacggga aatattcctg ggtgaaatca
    12301 ccgactttct acggcaaaac ggtagaagtg ggtccgctgg ctaatatgct ggtgaaactg
    12361 gcggcaggtc gcgaatctac ccagaacaaa ctgaatgaaa tcgttgcgat ttatcagaaa
    12421 ctgactggca acacgctgga agtggcacag ctgcactcca cgctgggccg tattattggt
    12481 cgtaccgttc actgctgtga attgcaggat atcctgcaaa accaatacag tgcactgatc
    12541 accaatatcg gcaaaggcga tcacaccacc tttgtgaagc cgaacattcc ggcaacgggt
    12601 gagttcaaag gtgttggctt cctcgaagcg ccgcgcggta tgctctctca ctggatggtt
    12661 attaaagacg gtatcatcag caactaccag gcggttgttc catcaacctg gaactctggt
    12721 ccgcgtaact tcaatgatga cgtcggtcct tacgagcagt cgctggtggg tacaccggtt
    12781 gccgatccga ataaaccgct ggaagtggtg cgtaccattc actccttcga cccgtgcatg
    12841 gcctgtgcgg tacacgtagt ggatgccgac ggcaacgaag tggtttcagt gaaggttctg
    12901 taatgcgtat tttagtctta ggggtcggca atattttgct gaccgatgaa gccatcggcg
    12961 tgcggattgt cgaagcgtta gagcaacgat acattctgcc ggattatgtt gagatcctcg
    13021 atggcggcac ggcgggaatg gagctgcttg gcgacatggc aaatcgcgat catctgatta
    13081 ttgcggatgc cattgtgtcg aaaaagaaca ctccgggaac gatgatgatc ctgcgggatg
    13141 aagaagttcc ggcgttgttt accaacaaaa tctctccgca tcagcttggc ctggccgacg
    13201 tattgtcggc cctgcgcttc accggtgagt ttccgaaaaa gctgaccctg gtcggcgtga
    13261 tcccggaatc gctggagcca cacatcggct taacgccgac ggttgaagca atgattgaac
    13321 ctgcgcttga gcaggttctg gctgcgctgc gcgaatcagg cgtggaagcc atcccacggg
    13381 aggcgattca tgactgaaga gatagcaggt ttccagacct ccccgaaggc gcaagtacag
    13441 gcagcgtttg aagaaattgc ccggcgttcg atgcacgatc tctcttttct gcatccttca
    13501 atgccggtgt atgtttctga ttttacgctg ttcgaaggtc agtggacggg gtgtgtgatc
    13561 accccgtgga tgctgagtgc agttatcttc cccggcccgg atcaactctg gccgctgcgc
    13621 aaagtgagtg aaaaaattgg tctgcaactg ccgtatggca ccatgacctt taccgttggc
    13681 gaactggacg gtgtttcgca atacctctcc tgttcgctga tgtcgccgct ttcgcacagc
    13741 atgtcgattg aagaaggcca acgcctgacg gatgactgcg cacgaatgat cctttcgttg
    13801 ccagtcacca atccggatgt accacacgca gggcgtcgcg ccctgctgtt tggccgcagg
    13861 aggggcgaaa atgcatgagt tgtcgctttg ccagagcgcc gttgaaatta tccaacggca
    13921 ggcggagcag cacgatgtta agcgcgtcac cgccgtgtgg ctggaaattg gcgcgctctc
    13981 ctgcgttgag gagagcgccg tccgttttag ttttgaaatt gtctgccacg gaacggtggc
    14041 gcaagggtgc gatttacata tcgtctataa acccgcccag gcctggtgct gggattgcag
    14101 ccaggtggtg gagattcatc agcacgatgc gcagtgtccg ctctgtcacg gcgagcggtt
    14161 gcgtgtcgat accggcgatt cgctgatcgt caaaagtatt gaagttgaat aagactgagg
    14221 acaacgccta aaaagcgcac ggacggtccc ctcgcccctc cggggagagg gttagggtga
    14281 gagggaacac ccgctaaagg ttctttttcg cgtttgtccg gcaatcgaaa taaccggagt
    14341 taacaatgtg tattggcgtt ccaggccagg tgctggctgt cggtgaagat attcaccagc
    14401 ttgcgcaggt tgaagtatgt ggtatcaagc gcgacgtgaa tatcgccctg atttgcgaag
    14461 gtaaccctgc cgatctgctg ggccagtggg tgctggtaca cgtcggattt gccatgagca
    14521 tcatcgacga agatgaagcc aaagccacat tagacgcact gcgccaaatg gattacgaca
    14581 ttaccagcgc gtgatgatta gcttttgtag gcctgataag acgcggcagc gtcgcatcag
    14641 gcattgtgca cgattgccgg atgcggcgtg aacgccttat ccggcctgga tggcttgctg
    14701 cgacgaacac caacccttac ccctgacgct tatcttctgt atttgtctca aaatcactgg
    14761 cgtcatggcg ctcatgcaac tgctcgttca gcggcccgtt ggtgcggtta acaatacgcc
    14821 cacgtttcac tgccggacgt tcgcccactt ctttcgccca gcgttgcaca tgtttataac
    14881 ttcccgcatc cagaaactct gcggcatcat acacgccgcc gagcaccacg ttgccaaacc
    14941 acggccaaat cgccatatcc gcaatggtgt actcatcgcc cgcaacaaac ttatgctgcg
    15001 ccagttgctt atccagcacg tcgagcaggc gcttggcttc catggtaaag cggttgatgg
    15061 cgtactcaat tttcaccggc gcgtaatggt aaaagtgacc aaaaccaccg ccgaggaacg
    15121 gcgcggcacc ctgtaaccag aacagccagt tcaacgtttc agtccgtttt gccagatcct
    15181 gcggcaggaa gtagccaaat ttctccgcca gataaagcag gatcgaacca gattcaaaca
    15241 cgcggatcgg cggattatgc gtatgatcgc gcagcgccgg gatcttcgag tttgggttca
    15301 cttcgacaaa gccgctggag aattgatcgc catcgccaat acgaatcagc caggcgtcgt
    15361 actctgcacc agtaacgccc agcgccagta gc
//
LOCUS       DAJTSI010000096        15175 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000096 DAJTSI010000000
VERSION     DAJTSI010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15175
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.096"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            529..1941
                     /gene="pykF"
                     /locus_tag="OO327_002712"
     CDS             529..1941
                     /gene="pykF"
                     /locus_tag="OO327_002712"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR6019196.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            2252..2488
                     /gene="lpp"
                     /locus_tag="OO327_002713"
     CDS             2252..2488
                     /gene="lpp"
                     /locus_tag="OO327_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR6019197.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(2551..3555)
                     /gene="ldtE"
                     /locus_tag="OO327_002714"
     CDS             complement(2551..3555)
                     /gene="ldtE"
                     /locus_tag="OO327_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR6019198.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(3704..4120)
                     /gene="sufE"
                     /locus_tag="OO327_002715"
     CDS             complement(3704..4120)
                     /gene="sufE"
                     /locus_tag="OO327_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR6019199.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            complement(4133..5353)
                     /gene="sufS"
                     /locus_tag="OO327_002716"
     CDS             complement(4133..5353)
                     /gene="sufS"
                     /locus_tag="OO327_002716"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR6019200.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            complement(5350..6621)
                     /gene="sufD"
                     /locus_tag="OO327_002717"
     CDS             complement(5350..6621)
                     /gene="sufD"
                     /locus_tag="OO327_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR6019201.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            complement(6596..7342)
                     /gene="sufC"
                     /locus_tag="OO327_002718"
     CDS             complement(6596..7342)
                     /gene="sufC"
                     /locus_tag="OO327_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR6019202.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            complement(7352..8839)
                     /gene="sufB"
                     /locus_tag="OO327_002719"
     CDS             complement(7352..8839)
                     /gene="sufB"
                     /locus_tag="OO327_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR6019203.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            complement(8848..9216)
                     /gene="sufA"
                     /locus_tag="OO327_002720"
     CDS             complement(8848..9216)
                     /gene="sufA"
                     /locus_tag="OO327_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR6019204.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            complement(9764..9952)
                     /locus_tag="OO327_002721"
     CDS             complement(9764..9952)
                     /locus_tag="OO327_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR6019205.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            complement(10052..10462)
                     /gene="menI"
                     /locus_tag="OO327_002722"
     CDS             complement(10052..10462)
                     /gene="menI"
                     /locus_tag="OO327_002722"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR6019206.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            complement(10459..13515)
                     /locus_tag="OO327_002723"
     CDS             complement(10459..13515)
                     /locus_tag="OO327_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR6019207.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            13904..15016
                     /gene="ydiK"
                     /locus_tag="OO327_002724"
     CDS             13904..15016
                     /gene="ydiK"
                     /locus_tag="OO327_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR6019208.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
BASE COUNT         3647 a         4018 c         3695 g         3815 t
ORIGIN      
        1 tcagcatata tcaatctaac caattaaaca aaaatcaaac aaaatcagac aaataacgcg
       61 ataaattatt ttaactgtag caattgagcg atgatatatt tatacaccgg atgaactttc
      121 acttatcctc acactgacaa cttcggcacc agacgttgcg caaacagcga agtttttgcg
      181 taaccttttc cctggaacgt taaatctttg ataacaattt attgtctaac aagttgtata
      241 ttttttgaaa cgctgttttt gttttccttt tggattaatt tcagcgtata atgcgcgcca
      301 attgactctt gaatggtttc agcactttgg actgtagaac tcaacgactc aaaaacagac
      361 actcacgttg ggctgagaca caagcacaca ttcctctgca cgctttttcg atgtcaccta
      421 tccttagagc gaggcaccac cactttcgta ataccggatt cgctttccgg cagtgcgccc
      481 agaaagcaag tttctcccat ccttctcaac ttaaagacta agactgtcat gaaaaagacc
      541 aaaattgttt gcaccatcgg accgaaaacc gaatctgaag agatgttagc taaaatgctg
      601 gacgctggca tgaacgttat gcgtctgaac ttctctcatg gtgactatgc agaacacggt
      661 cagcgcattc agaatctgcg caacgtgatg agcaaaactg gtaaaaccgc cgctatcctg
      721 cttgatacca aaggtccgga aatccgcacc atgaaactgg aaggcggtaa cgatgtttct
      781 ctgaaagctg gtcagacctt tactttcacc actgataaat ctgttatcgg caacagcgaa
      841 atggttgcgg taacgtatga aggtttcact actgacctgt ctgttggcaa caccgtactg
      901 gttgacgatg gtctgatcgg tatggaagtt accgccattg aaggtaacaa agttatctgt
      961 aaagtgctga acaacggtga cctgggcgaa aacaaaggtg tgaacctgcc tggcgtttcc
     1021 attgctctgc cagcactggc tgaaaaagac aaacaggacc tgatctttgg ttgcgaaaaa
     1081 ggcgtagact ttgttgctgc ttcctttatt cgtaagcgtt ctgacgttat cgaaatccgt
     1141 gagcacctga aagcgcacgg cggcgaaaac atccacatca tctccaaaat cgaaaaccag
     1201 gaaggcctca acaacttcga cgaaatcctc gaagcctctg acggcatcat ggttgcgcgt
     1261 ggcgacctgg gtgtagaaat cccggtagaa gaagttatct tcgcccagaa gatgatgatc
     1321 gaaaaatgta tccgtgcacg taaagtcgtt atcactgcga cccagatgct ggattccatg
     1381 atcaaaaacc cacgcccgac tcgcgcagaa gccggtgacg ttgcaaacgc catcctcgac
     1441 ggtactgacg cagtgatgct gtctggtgaa tccgcaaaag gtaaataccc gctggaagcg
     1501 gtttctatca tggcgaccat ctgcgaacgt accgaccgcg tgatgaacag ccgtctcgag
     1561 ttcaacaatg acaaccgtaa actgcgcatc accgaagcgg tatgccgtgg tgccgttgaa
     1621 actgctgaaa aactggatgc tccgctgatc gtggttgcta cccagggcgg taaatctgct
     1681 cgcgcagtac gtaaatactt cccggatgcc accatcctgg cactgaccac caacgaaaaa
     1741 acggctcatc agttggtact gagcaaaggc gttgtgccgc agctggttaa agagatcact
     1801 tctactgatg atttctaccg tctgggtaaa gaactggctc tgcagagcgg tctggcacac
     1861 aaaggtgacg tcgtagttat ggtttctggt gcactggtac cgagcggcac tactaacacc
     1921 gcatctgttc acgtcctgta atattgcttt tgtgaattaa tttgtatatc gaagcgccct
     1981 gatgggcgct ttttttattt aatcgataac cagaagcaat aaaaaatcaa atcggatttc
     2041 actatataat ctcactttat ttaagatgaa tccgatggaa gcatcctgtt ttctctcaat
     2101 ttttttatct aaaacccagc gttcgatgct tctttgagcg aacgatcaaa aataagtgcc
     2161 ttcccatcaa aaaaatattc tcaacataaa aaactttgtg taatacttgt aacgctacat
     2221 ggagattaac tcaatctaga gggtattaat aatgaaagct actaaactgg tactgggcgc
     2281 ggtaatcctg ggttctactc tgctggcagg ttgctccagc aacgctaaaa tcgatcagct
     2341 gtcttctgac gttcagactc tgaacgctaa agttgaccag ctgagcaacg acgtgaacgc
     2401 aatgcgttcc gacgttcagg ctgctaaaga tgacgcagct cgtgctaacc agcgtctgga
     2461 caacatggct actaaatacc gcaagtaata gtacctgtga agtgaaaaat ggcgcacatt
     2521 gtgcgccatt tttttgtctg ctatttacca ctactgcgtc gcgcgtaaca tattcccttg
     2581 ctctggttca ccattctgcg ctgactctac tgaaggcgca ttgctggctg cgggagttgc
     2641 tccactgctc accgaaaccg gataccctgc ccgacgatac aacgctttat cgactaactt
     2701 ctgatctaca gccttattgt ctttaaattg cgtaaagcct gctggcagtg tgtatggcat
     2761 tgtctgaacg ttttgctgtt cttctgctga tagtggtcga tgtacttcaa cataacgcat
     2821 cccgttaggt tccacggaat atttcaccgg ttcgttgatc actttcaccg gcgttcccgt
     2881 ccgcacgctg gagaacaagg ctttaatatc cggtgcattc atgcgaatac accctgaact
     2941 gacgcgcaaa ccaacgctgt ccggcgcact ggtgccatga atgaggtatt cgccattacc
     3001 atgcgcgagg cgcagtgcgt aacgtcctag cgggttattt ggtccggcag gaacgactgg
     3061 cggtaattta atgccacgct ccagcgaacg ctgacgaatg cctgccgtag gcgtccaggt
     3121 tgggttaggg attttctgcc caacacgcgt ttccatcacc ggcgtttcca gcccctgcaa
     3181 tccaatacct attggataaa cctgcacaat attttctccc ggcggataat aataaaggcg
     3241 cagctctgca aggttaacga taatcccctg acgcggtgca tcaggtaata acagttgtga
     3301 aggaatagtt atcgtcgtac caggttttgg caccggggcg atagtgttat tggcttcaag
     3361 gatcaacatt gctgcagtat caaaacgtcg ggcaatagcc tgaaggtttt tatccccttc
     3421 ttgcaccgta tacgtttgat tttgcccaac cagtcggctt ccggttggtg gtagcggata
     3481 atcaaccgcc caggcagcct ggatggcgct aaaagcgccg ataagcgtga gtgtaagcaa
     3541 agacgcgcgt ttcattgtaa acctcctgta tttgccggag actcacgctg aaacgtcgga
     3601 tggtgcttat gttcacctga aaccaaaaca ctcctgtgcc ggtcagtgta aacattgacc
     3661 atccggcaat gtgagccaac cggatgaaag ctgtcccttt agtttagcta agtgcagcgg
     3721 ctttggcgcg aattgcgcga atcatcgctt ccagaccttg tgaacgagat ggggtgagat
     3781 gttgggtgag cgccattttt tcaaaccacg gacgcacatc gaaattgaca atatcctgcg
     3841 gcgtcatctg atcgtagaga ataaagacga ccgcaataag ccctttcaca atcgccgcat
     3901 cgctgtcgcc ctgcaattca ataattccct gcgcattctg gcgcatgaca atccacacct
     3961 gactctggca gccctgaatg ctattttgtg ggcttccgtc ttcggcgcgt aattctggca
     4021 gacgctggcc cagctcaata atgtagagat atttctcttc ccagttggcg cagcgtaaaa
     4081 aattacgcag caatttttct ttatccggca ataaagccat agtgcctccc tgttatccca
     4141 gcagacggtg aatacgttgc aggccggtca ccagacgctc cacttcttca tgggtgttat
     4201 acatggccag cgacgcccga cacatcgcag ggacgttgta ataggccatt aatggcatag
     4261 cgcagtgatg tccggtacgc acagcaatgc cgtaattatc gagaaaactg ccaacatcat
     4321 aggcgtggtg tttaccgaga ttaaaagcaa taacgccaag cctgttttgt tggccataga
     4381 gagtgagatc cggtacagat tccagctgtg atagcgcata atgcatcaga ttctgttcat
     4441 actcggctat gctattaagc cccagcgccg aaacatactc cagcgccgcg ccaagaccaa
     4501 tgatgccccc ggtattgggt gtaccggctt caaaccgcca tggtgctttg gtccaggtag
     4561 tgccttcact caggctgacg gtggcgatca tagaaccgcc cccttcccac ggcggcatct
     4621 cctgcaacaa ggcttctttc acataaagaa tgccaattcc ggtggggcca tacagtttat
     4681 gcccggagaa cacgtaaaag tcgcaatcca gcgcctgaac atccaccgga tgatgcatca
     4741 ccgcctgagc gccatccacc agcacttttg cgccatgctg gtgcgcaagc gtgatcattt
     4801 ccgccagtgg attttctgtg ccaagcacgt tggagacatg agtaattgcc agcaggcgag
     4861 ttttctcatc aaacagcgta ggcagcgtct ccagttgcag cgtaccgtcg gggttgagag
     4921 ggatcacacg cagctctgcg ccaacgcgtg cgcaaagcat ctgccagggg acaatgttag
     4981 cgtggtgctc catctgactg atgatgatgt tatcgcccgc ccgcacattg ctgttgcccc
     5041 agctattggc gaccagattg atcccttccg tcgtgccgcg gacaaacacc agctcttccg
     5101 ccgaacgggc attaataaac agcgatgccc gcttgcgcac gttctccatt ttctcggtcg
     5161 cctgggcgct taaggtatga ataccgcgat gcaccgccgc gtagccatga cgataaaact
     5221 cggcctcggc gtcaatcacc tggctcggtt tctgtgcact ggcggcgctg tcgagataag
     5281 ccagcggcaa accgtttacc tcacgcgaaa gcaccggaaa gtcggcccgc actttgtcga
     5341 cggaaaaagt catcttgcac ctcctggcag ccgttgaccg attcgggcca gcacctgctg
     5401 tttaagcccc tcatcacgca gtgcttccgt cagttcggca gcaaaggcgt aaatgatcat
     5461 ctgctgggca tcctgctgat tgatcccgcg cgagcgcaga tagaacatct gttcatcatc
     5521 aatacgcccc accgtcgcgc cgtggctgca tttcacatca tctgcataga tttccagctg
     5581 cggttttgta tccacttccg ccagtttgcc catcagcaga ttattgttgg tcatctgacc
     5641 atccgttttg atggcgtgct gcgcgacgtt gatcaaaccg ttaaataccg cgcggccttt
     5701 gtcgctgacg atagttttgt gcaactgtcg gctgttacaa aaacctttat tgtgttccag
     5761 ccaggtacgg gtatcacaca cctcgttttt caccggcatc gccaggctat tgatccgcag
     5821 cgtgctgttt tcgccattga gttgcgtact ggtgttgtgt cgtaacactg cgccacccag
     5881 caggaaacta tggctaaatg cggtggcatc gtcagccagc aacaaatcgt tatgggcaaa
     5941 gtggtgactg accgggtttt caaacgccag cttgatatgc tgcaagtggg cgttcgctgc
     6001 gacattgata gtgaaccgtg ccccggtaaa atgacgagca tcattaaggc tgacaaaatg
     6061 ttcgatcacc gttgcttcgg caccttccgc cagatccaga tgatgccggt aatgggcagt
     6121 gttcacctct tcacctgcca cgccctgggt gatatgcatt aacagcaatg gctttgccgg
     6181 ccgttgaccg cgcttcacgg cgatatgcgt cacgctttgt gccaggcttt ccgtcaaatg
     6241 cagaaacact tccgcctgaa tagcgtcggg taacccctga cggtcgtcgt taatgctcac
     6301 ttcatatccg ctgccttcaa ttgcatcgct cagcgcgggc acgtaacgtc catcgacaaa
     6361 caccagccgc acggcgtcta acgttaacgc taaggcatca cgctgctgtg gggatatatc
     6421 tcccgcaatg ctgacaaact gactattggt cagcccttcc agcggcgtat atttccagtt
     6481 ttcatgttta cgtgtcggca gtccggtacg cagcaattgt tgtaaatgct gctgtgcttg
     6541 cggtgagcgt tttgtccctt cagcttcaaa caagtgatgc cactgttgca gcgcattact
     6601 gctgttcggt aagccagcca taaccctgct cctccagttg tttgaccaac gtgaaatcgc
     6661 cggatttcac aattcgtccc tgatatagca catgaacgta atcaggcttg atgtagtcga
     6721 gaatgcgttg gtagtgcgta acaatgatga atgagcgctt gccatcacgc agcgagttca
     6781 cgccatcggc gaccactttt aatgcgtcaa tatccagccc ggagtccgac tcatcaagaa
     6841 tgcataactc cggttccagc accgccattt gcaaaatatc gttgcgcttt ttctcgccgc
     6901 cggaaaaacc aacgtttacc gaacgggtta ataaatcttc cggcatcttc aggagagcga
     6961 ttttctcttc catcaaatcc tgaaaatcaa agcggtcgag cgtttcctgg ccgcgatagc
     7021 tgcgcactgc attaagtgcc gtttgcagga aaaactggtt actgacgcct ggaatctcca
     7081 ccggatactg gaaggccata aagatgcctt cgcctgcacg atcttccggc gacagtgcaa
     7141 gcaaatcttt gcctttgaac tcaaccgtgc cgcccgtcac ttcataatct tcgcgcccgg
     7201 caagcgttgc cgataaggta cttttgcccg aaccgtttgg ccccataatg gcgtgaactt
     7261 cgccgggacg aacgtcgagg cttaatccac gcaggatagc tttatcttcc acgctgacgt
     7321 gtaaatcttt aatacttaac atgtttattc cttatccgac gctgtgttca agactgatgg
     7381 cgaggagttt ttgtgcttca acggcaaatt ccagcggcag ttctgagaac acgtctttgc
     7441 agaaaccgtt aacaatcatc gagatggcgt cttcttcgct gatcccgcgt tgcaggcagt
     7501 aaaacagttg atcttcacca atacgtgatg tcgttgcctc gtgctccagt tgtgcgctgt
     7561 tattgcgaca ttcgacatac gggaaggtat gcgccccaca attagcgcca atcagcattg
     7621 agtcgcactg agtgaaattg cgcgcattgg ttgccgtcgg catgattttc actaagccgc
     7681 gataactgtt ctgactatgt ccggcagaga tccctttcga gataatggtc gatttggtgt
     7741 ttttaccgat gtggatcatc ttggtgccgg tatccgcttg ctgatgaccg ctggtcagcg
     7801 ccactgagta aaactcacca atggagttat cgccgcgcaa aatgcagctg ggatatttcc
     7861 acgtaatcgc tgacccggtt tctgattgcg tccatgacat tttgctgttt tcgccttcgc
     7921 acaaagcacg cttggtgacg aagttgagaa taccgccggt gttgttatcg ccagggaacc
     7981 agttttgtac cgtggaatat ttcacctcgg cgtttttatg gatgatgact tccaccactg
     8041 ccgcgtgcaa ctgatagctg tcacgcaccg gagcggaaca gccttcaatg tagctgacgt
     8101 agctgtcttc gtcggccacc agaattgtgc gctcaaactg cccggttttt tccgcgttaa
     8161 tgcgaaaata ggtggaaagt tccatcgggc agcgcacgcc tttaggcaca taaataaacg
     8221 taccgtcaga ggctaccgcc gcattaagtg ctgcaaagaa gttgtcattc cccggcacca
     8281 cggtgccgag atatttacgc accagttccg ggtgatcgtg gatcgcctca ccaaaggaac
     8341 agaaaataat tccctgctcc gccagttttt cgcgataagt ggtggcaacc gaaacagagt
     8401 cgaaaatggc atccaccgcc acctctttgc cttcccgcac gggaacgccc aactgctcaa
     8461 acgccgcctc cacctcttta cttaaaaagg cgttcgcgcc agtttgctgc accgcgccag
     8521 gttcagacgc gcaagtgtcg tcacaattac cgcacgatgg tgctgagtag tagctgtaat
     8581 cctgataatt cagcttgtcg tagtgcgctt tcaaccagtg cggttcttcc atctccagcc
     8641 atgcgcgata ggcgtttaga cgaaactcca gcatccactc cggctcatta cgcttcgccg
     8701 aaattgcgcg caccacctct tcgtttatcc cctttgccag ctcatcggtg gctaactggg
     8761 tgaagaatcc ttctttatag ttcagcgggc cgccggtcca ggttttgaca tcgtcagttg
     8821 cttcagtatt acgagacata gtaccgccta taccctaaag ctttcgccac agccacattc
     8881 attctgggct ttagggttgt gaaatttgaa tatctgatta agtccttcac gaacgaaatc
     8941 gacttccgtg ccatcaataa acggcatcga ttgcagcggg acaaacagct tcgcgccgtc
     9001 gtgttcaaac agcagatcgt ctttgtccgg ctcgctaaca ctgtcgagca catagccaaa
     9061 gcccgcgcag cccgtttgct tcacacctaa gcgcacgccg accatacctg gctgctttgc
     9121 caccagctcg cggatgtgta tcgccgctgc gggtgtcagc gttaagcctt gccaggcgaa
     9181 atcctgtggg ttaaaggttc ctgaatgcat gtccatctat ttacctcact tcatcgcttt
     9241 cagcgtataa cagcatgtta gtgataatga ttatcagttc aacccagcaa acgcaggggc
     9301 tttaccgtaa aacattcttt ttgcctgctt ttaataagca tagcccctga tgtgtgggtt
     9361 aacaggcacg ctaagaatta ggtatctcat tgttagataa tggttattat ctaaggagca
     9421 ttaaggctgt gatgaaaaag aaaaagttgt attgaaaatg actatttaag agataggtaa
     9481 aaaagtgcag cgttcagaaa taagaaaacc cttaagtctg tgcgacacag gcttaagggt
     9541 ttctacccca tccggcgctt atctccggca ctctcagtgg cttagctctt gaaggggcga
     9601 taagaataat ctcataaagc taacccgccg ttttaacaca aactgcgatt agtattattt
     9661 ttgaacaata tcaggcggta gataagcagt attaagaagg tcatcgaacc tggacggagg
     9721 ttaatccagg tcgatttggc gaacttgcgg cattaagtca ggatcaatgc acgcccagac
     9781 gccaggcgaa gtagatttct tcttttaatt cagcagaaga gagagtaagc aggtcagcaa
     9841 attcaagttc tagttgtttc agacgtttga gatattgggc aggtgaaaga ttgctctggt
     9901 cacggcgtaa aaattcaatg gccagctggg tgggatcaag ttgagtagac atagcatcct
     9961 cgcttttaga caagacctgc acagtatacc accgtttact gtgcagataa tgaccaaaag
    10021 caatatgcgt cacacttttc tggtgacaac gtcacaaaat ggcggtcgtc aatcgtgacg
    10081 aacagcacaa acgccctttc tcatcgaaga tttcaatctg ccagacctgg tgacgcgaac
    10141 cgagatgcaa cggtttacat atgccgcgca cccgcccttc tcgtgccgag cggacgtgat
    10201 tagcattgat ttccagacca accacttttt gctcaccttc ggtacataaa taaccggcaa
    10261 cggaaccgat actttcggcc agtaccacgg atgctcctcc atgcagcaac ccgaaaggct
    10321 gctttgtccg cgagtctact ggcattgtcg cttcaagggt gtcatcacca atatgttcaa
    10381 agcgaatatc caggaacccc accatgtttc cttcacccat agcattcagt gcttccaggg
    10441 tgattttccg tttccatatc atttaataat ctccagtaaa gcctgcacag gatggcgtac
    10501 ccccgtgcct tcaacccgtt ttacctggct acggcaggaa tatccggtcg ccagacagcg
    10561 gttacgcggc agtcgctgca tagcctgatg ccaggataac tcatagatcc cgagcgaatt
    10621 ttcatggttt ttcgcttcat gtccgtaagt ccctgccatg ccgcagcaac ccacgctgac
    10681 attttccagt ttcgcgccaa aacgggcaaa tatcgcggcc cattgtgctg gcgcgcccgg
    10741 caaagcggta acctcggtac agtgaccaaa gaaataccat gattcaccgc tgactgtagc
    10801 cactggatgt gactcaagtg cgcttgccag ccattcattc gcaagtaaga cgttaaactc
    10861 gccacgttcc tcgcccagcg ccagtttata ttcatcgcga taacaaagta ccagcgccgg
    10921 atcgacgccc accattggca tcccaagctt agccatacgg ttgaggaaat ccgccgtctt
    10981 tttcgccgtc ttcgcaaaac gattaagaaa gcctttgata tgctgggctt tgccatttgg
    11041 cgaaaatggc agtaacacag gctggaaacc taatttttca accagacgga caaaatccgc
    11101 caccacttgc gcatcgtaat agctggtaaa ggggtcctgc accaccaaca ccgtgcgcgc
    11161 tttctgctct gcattgagag cttcaagctg ttccagcgtc atgtttgccg agcgatgccc
    11221 caccatttgt tgttgtagcg aggggaccga cagcagtggc aaatcaacca tgccgatatg
    11281 tttttccgag agtttgcgca ccagcggctg gttaatgaag aagttaaagg ttttcggcgc
    11341 gcgtgccatc agcggtgcgt agctctcgac cgtagcaacg aggtggtcgc gaagcgggcg
    11401 taaataacgg gtgtgataga gctgcagaaa acgagagcga aactccggca catcaatttt
    11461 gatggggcac tgggtcgaac aagctttaca ggccagacag cccgacatcg cctctttgac
    11521 ttcgtgtgag aagtcatatt cgcctttatt cgcatgccag ctattgcgcg tgcgggcaat
    11581 taacgtccgc aaactgacgc cggattcagg cagttcttgt tccagtttga gtgggtcaac
    11641 gccgcgatcc gccaacaagc gcagccattc acgcaccagc gttgcgcgcc ctttcggtga
    11701 atgaatccgg ttctgggtga tcttcatcga cggacacatc ggactacggg catcaaagtt
    11761 gaagcataaa ccgttgccgt tacactccat cgcaccgcgc cactgctggc gtaccgcaat
    11821 ggggatctgc cgatcgaatg taccgcgctt caccgcgtcc actttcatca tcggcgcatc
    11881 gagaccttct ggcgggcaaa tcttccctgg gttgagtcgg ttatgcgggt caaatgccgc
    11941 tttcactttg cgcagttctg caaaaagttc ctcaccgaaa aacgccgggc tgtattcagc
    12001 gcgaaaacct ttgccgtgct cgccccacaa caaaccaccg tatttcgcag tcagcgccac
    12061 cacgtcatca gagatttgct tcatcaaaat ctcttgttgc ggatcgcaca tatccagtgc
    12121 cggacggacg tgcaagacac ctgcgtcgac gtgaccgaac ataccgtagc ttaagccgtg
    12181 gctgtcgagc agcgcgcgaa attcagcaat ataatcggcc agatgttccg gcggtacgca
    12241 ggtatcctca gcaaacggaa ttggcttagc ggcacctttg gcattgccaa gcagaccaac
    12301 ggcttttttg cgcatcgcat agatacgttc aacccccgcc agctcgcggc acacctgcca
    12361 gccaatcaca cctgcctgtt ggctggcaat cagctcatca agccgcacac agagtgcatt
    12421 aactcgctcg tcaatcagcg cctcatcatc accagcaaat tccacaatgt tcagtccgag
    12481 catctctttg tcaggcatat cggtaatcaa ctcgctgacg gaatgccaga caatatcttc
    12541 ccgcgccaga ttcagcactt ttgagtccac cgtctctacc gaaagcgccc gcgcctcaac
    12601 cataaacggc gcgttacgca gcgcagagtc aaaagagtca tatttgacat tcaccagacg
    12661 gcgcacttta ggcaagcgcg taatatccag ccgcgcttcg gtaataaagg ccagcgtccc
    12721 ttctgaaccc gtcagaatgc gcgtcaggtc gaactcggtc atctcatcgt taaagacatg
    12781 acgcagatcg taaccggtaa gaaagcggtt aagtttgggg aagttgtcga tgattaactg
    12841 gcgttgctga cggcaacgtt gataaaccgt gttataaatt cgcccgattg tggtattgga
    12901 tttacccagc gtttccgcca gctcgacggg taaaggttgc gtatcgagaa tatcgccccc
    12961 caacaacacc gcgcgtacgc caagtacgtg atctgacgtt ttgccataga ccagcgatcc
    13021 ctgaccggat gcatcggtat tgatcatccc accgagcgtt gcccggttgc tggtcgaaag
    13081 ttccggcgca aaaaagtagc cgaacggttt caggtactga ttgagttggt cttttatcac
    13141 cccggcctca acgcgcaccc agccctcttc agggttaatt tcgatgatgc ggttcatatg
    13201 gcgggacata tcaacaataa tcccctggtt gagcgcctga ccgttagtgc cggtgccgcc
    13261 gccgcggggg gtaaagatca gcgatgaata gcgttcctgc gcggcaagac gggcgatcag
    13321 cgccacatct gcggttgaac gcggaaatac caccgcatcg gggagaagtt ggtaaatact
    13381 gttgtcggtc gacattgtca gacgatcggc ataacttgtc gccgcatcgc cggtaaaacc
    13441 ttgttgctcc agctcttgca aaaaattaag caccagttga acgacgccgg gtgccaggga
    13501 aatctgtgga atcattatat tgaccctttc ctgcggtctg tgatgtaggt cgatacacta
    13561 ttctttcagg cggctgcaat agcgcactga aaggtgatgt ttatttactc tatggatttc
    13621 gagttgcagg aaggcggcaa gcgagtgaac tccaggagct tacaatagta agtgactggg
    13681 gtgaacgaac gtagccgcag cacatgcaac ttgaaatacg acgagtaaat cgtttgcgtg
    13741 ttgcctgagt tgttgtacca catttttttc taacacgccc atcagaatta agggcagaat
    13801 cggcctgtta aaaaccgctg aaattgctca tcattatgca ggtgagtttc gcgtgttcac
    13861 gtcgcgtcga cgatttgacg cacaaaaaag gtgaaaagta gttatggtaa atgttcgtca
    13921 gcccagggat gtcgcacaaa ttctgctttc ggtgctgttt ttagccatca tgattgtggc
    13981 atgtctgtgg attgttcaac cctttattct cggctttgca tgggccggta cggtggttat
    14041 cgccacctgg ccggtattgt tacgtttgca aaagatcatg tttggccgcc gctctctcgc
    14101 cgttctggtg atgacgctgt tattagtgat ggtgtttatc atccctattg ctttgctggt
    14161 taacagtatc gtcgacggca gcggcccgct aattaaagcc atttccagcg gcgacatgac
    14221 gttacccgat ctggcgtggc ttaataccat tccggtgatt ggcgcgaagc tgtatgcagg
    14281 ctggcacaac ttgctggata tggggggtac ggcgatcatg gcgaaagtcc gcccttatat
    14341 tggcaccacc accacctggt tcgttgggca ggcggcgcat atcgggcgct ttatggtgca
    14401 ttgtgcgctg atgcttctct tcagtgccct gctgtactgg cgcggtgaac aggtggcaca
    14461 aggcattcgc cattttgcaa cccgtctggc aggcgttcgc ggagatgccg ccgtgctgct
    14521 ggcggcacag gctatccgcg cggtggcgct gggtgtggtg gtgacggcgt tagtacaggc
    14581 agtgcttggc ggtatcggcc tcgccgtatc cggcgtacct tatgcaacgt tgctaacggt
    14641 gttaatgatc ctctcctgcc ttgtccagct tggcccgttg ccggtactga ttccggcgat
    14701 tatctggctc tactggaccg gcgataccac ctggggaacg gtattgttag tgtggagcgg
    14761 tgtggttggc acgctggata acgtcatccg cccaatgtta attcgcatgg gtgccgattt
    14821 accgctgatc ctgatcctct ctggcgttat tggtggtttg attgctttcg ggatgatcgg
    14881 tctgtttatt ggtccggttc tgttagccgt ttcctggcgt ctgtttgccg cgtgggtgga
    14941 agaagtcccg ccaccgacgg accaaccgga agaaattctc gaagaacttg gcgaaatcga
    15001 gaaaccgaat aagtaatttc tcatcaggcg gctctgccgc ctgattgtta atcaccgcta
    15061 attgatattt gcttgctctt ccccatattt tcctgcttac cgccattcag ctggtagtac
    15121 ctgtcgcaaa ttctttacag tttttaaact aatgagacga atctgatcga cgcaa
//
LOCUS       DAJTSI010000097        14980 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000097 DAJTSI010000000
VERSION     DAJTSI010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14980
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.097"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(523..1464)
                     /gene="fabY"
                     /locus_tag="OO327_002725"
                     /gene_synonym="yiiD"
     CDS             complement(523..1464)
                     /gene="fabY"
                     /locus_tag="OO327_002725"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR6019209.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1509..1946)
                     /gene="dtd"
                     /locus_tag="OO327_002726"
     CDS             complement(1509..1946)
                     /gene="dtd"
                     /locus_tag="OO327_002726"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR6019210.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1943..2815)
                     /locus_tag="OO327_002727"
     CDS             complement(1943..2815)
                     /locus_tag="OO327_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR6019211.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2809..3408)
                     /gene="yihX"
                     /locus_tag="OO327_002728"
     CDS             complement(2809..3408)
                     /gene="yihX"
                     /locus_tag="OO327_002728"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR6019212.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3507..4292)
                     /locus_tag="OO327_002729"
     CDS             complement(3507..4292)
                     /locus_tag="OO327_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6019213.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4326..5222)
                     /gene="yihV"
                     /locus_tag="OO327_002730"
     CDS             complement(4326..5222)
                     /gene="yihV"
                     /locus_tag="OO327_002730"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCR6019214.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5390..6286
                     /gene="yihU"
                     /locus_tag="OO327_002731"
     CDS             5390..6286
                     /gene="yihU"
                     /locus_tag="OO327_002731"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCR6019215.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6310..7188
                     /gene="yihT"
                     /locus_tag="OO327_002732"
     CDS             6310..7188
                     /gene="yihT"
                     /locus_tag="OO327_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCR6019216.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            7205..8446
                     /gene="yihS"
                     /locus_tag="OO327_002733"
     CDS             7205..8446
                     /gene="yihS"
                     /locus_tag="OO327_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCR6019217.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTVYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8584..9486
                     /locus_tag="OO327_002734"
     CDS             8584..9486
                     /locus_tag="OO327_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HCR6019218.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            9685..11721
                     /gene="yihQ"
                     /locus_tag="OO327_002735"
     CDS             9685..11721
                     /gene="yihQ"
                     /locus_tag="OO327_002735"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HCR6019219.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11767..13141
                     /locus_tag="OO327_002736"
                     /pseudo
     CDS             11767..13141
                     /locus_tag="OO327_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13184..14587
                     /locus_tag="OO327_002737"
     CDS             13184..14587
                     /locus_tag="OO327_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6019220.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14655..>14980
                     /gene="ompL"
                     /locus_tag="OO327_002738"
     CDS             14655..>14980
                     /gene="ompL"
                     /locus_tag="OO327_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HCR6019221.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYL"
BASE COUNT         3675 a         3854 c         3736 g         3715 t
ORIGIN      
        1 aattgattta atagcgtttg cttcggtgat ttattactca ttagttgttt ttctaaatta
       61 acaacagaat aactttcttg tttaatgaga ttaatattgg caaccattat agcctccttc
      121 ccacggttta agagcaaaca tacaacataa tgattcataa catacagagc agtaaatgaa
      181 tgatactgac aatgtatgcc agcccattat ctttgcataa tatttgtgag ctatagattt
      241 taaaaatatt tatgaaaaaa gtatggcacc agagggggtt ttgactatca aaaatcgctc
      301 aaacctgaac taatatgatg agttaagcat tatctgcaat aattgccact cagctctaca
      361 aataatattt aaatgctcct gaacagtgat gtagcaagac acgtatggtg ccaacgaaga
      421 ataaaaattt ataaagttct tattcaatat aaccggaata gtcagggact gatgtaaatg
      481 accggaatga atgagagagg cagcaccgcc cctctccata agctactctt cttcgttccc
      541 gccctcttca tacgggccaa atggcttcgc gggcagaacg atatacgtgc cttcaaacac
      601 tgcacccggc gtctcgtcgc caaagatttc gacctgcatc tgcacccgtg cttttcgtcc
      661 gcgcgccaga cggtcgagat cgccgcttaa ggcaccaagg tcggctaccg catggggttt
      721 accgctaatc ggtttgctgt agcggatatg cgcatccgcc agaataatcg ttccgccgag
      781 gtggcgttca cgcagcatca gccagataag cccccaaccg gtgagcgtcg ccagcgagaa
      841 taaactcccg gcaaacagcg tatggtgcgg attctgattg ccggtttccg gcatggtggt
      901 gataaatttt tgcccggtat attgttgaat acgcacgccc attttttcac taagcgggat
      961 atgttcgtac cacgcctgtt gcagctgcgc gcaccagtcg ccgcgatgca aaatatcatc
     1021 cagagtggcg acgggcttaa tcatcaaaaa atggcgaatc ggcgtggtgg ttggcgtggt
     1081 gatttctccc tgattaacaa accccagctt ggcgaaaaac tccaccgcgt cttcacgggc
     1141 gctacaggtc acgcgcttaa cgccttcctg acgcgccacc gactccaggg tcatcgccat
     1201 cagcgttcct aaccctttgt cctgcacgtc cgggtgaacg accataaagc gaatggacgc
     1261 ttcattgtcg gcattaatat acaatcggcc taccgccacc agattaccct gctcgtcgac
     1321 gaccatctgg tgatgcgcca tcgcatccca cgcgtcgcgt tccgaacctt ttggttgatg
     1381 caggggctta cgcaacattt cccagcgaaa gtgatagtaa cgctctaact cttcttctgt
     1441 ttgtggaacc tgaaggtgat acatagcgat actctctctt gtttcccgtg acaaccctgg
     1501 aagctggctc atacctgcaa ccagaatgtc acggggccat cattgaccag cgatacctgc
     1561 atatcggcag cgaagcgtcc tgtttgcgtg ttcatctctt gctggcggca gcgttcgacg
     1621 aaatagtcat ataacgcctc tgcgcgatcc ggtgatgcgc ctttggagaa acttgggcgc
     1681 atcccccgtt cggtatctgc ggcgagggta aactgggaaa ccaccagtac actgccgccc
     1741 gcctgttgca cgttgagatt catcttgcct tcggcatcgc taaagatgcg gtagccgagc
     1801 acacgctcgc acagacggtt tgctttctgt tcgtcgtcat ccttttcgac acccaataac
     1861 accaaaagtc ccgcgccaat ttcgcccgtc acttctccct ccacggtgac gctggcacgg
     1921 gttacgcgtt gaattaatgc aatcatggtt cgtcgtcttc ttcttgttca gctgcttgtt
     1981 ttagtttgcg gtattccccg agagtgacag taatttccgc gccaagcaag acgatacacc
     2041 atgtccagta gacccaaaca aagagaatgg ggatcaccgc cagcacaccg taaatgagct
     2101 gatatgacgg gaacatggtg atataaagcg cgaaaccttt ctttcctgct tcgaacagga
     2161 gtgcggcgac aaacgcgccg acaatcgcgt cgcggttagg tacgcggatg gtaggaacaa
     2221 tgctgtacag caaccagaag gagatccacg acaacagcaa cggaaaaata cgcagcacgt
     2281 tgtcgatgac agtattgaga tcgctcgccc agcgcaggga gagcaaatag gaactgatcg
     2341 ccagactggc ccctgccagc agcggcccca gcgttaaaat catccagtac acggcgaacg
     2401 agtaaatttt tggtcgcgct cgtttactgc gccagatggt attcaacgcg ctatcgatgg
     2461 agtacatcaa caataacgcg gtgacgatca gcccgcacgc cccaacggcg gtcatcttgt
     2521 tggaattagc aacaaattgt tcgatatacc gctgaataac atcgccagta gcaggcagaa
     2581 agttggcaaa aataaagtga cgtaactgaa tgctgacgtc ggaaaacatg ggaaaagcgg
     2641 cgaaaagcgc aaaaacaacg gcaaccagcg gcactaatga gagcaacgac acataggcaa
     2701 ggttacctgc cagggttgtc atgttgtcct catcaatacg ttgccagagt agttttagcc
     2761 aggcccatag tggacgggta cggtgcctgg ctttgtcctg aatggttttt agcataacac
     2821 cttcgcgaaa tagtccggga tggtggtttt atctttcacc agaatactgg taatgcctag
     2881 ctgattagct ccttctatat tatcggcgtt atcgtcgaaa aagaccgtat cgctgggtga
     2941 aaaaccttcc gcctgcaaaa catgctggta aattcgtgct tcaggtttgc gcatccccag
     3001 atcttgcgac agatagatat ggtcagcagc atcacgaatt tccgggtatt cttccggcca
     3061 gaaggtggta tgcaggcggt tggtattgga aagcaccacc acgcgatgcc cctgctcacg
     3121 cagtttatgc atgatggcga tcacttccgg gcgcagcgca acaaacaccg cctgccagcc
     3181 gtgagagaac tgctcgtagc ttagcggtag agccatctca tgacacagcg cctctgcgaa
     3241 cgcttcgtcg ctaatttccc cacgctcatg ctgatgaaac gcctccccca tatgaaaact
     3301 cttcttaagc gatgccagcg gaatacgcgt taaatcgctc caggctccca gcacacggtt
     3361 aaagtcgata tcgacaatca cattacctaa atcaaagata tagagcattt ttgcctcctt
     3421 tcgcgccaca agaaattaac tgtagcggga aaggatgggt ttggctatgt ggcaaggtat
     3481 gaagccaggt taacggtcac aaaaatctac gcgtcttcct ggggaagaat gatttcaatt
     3541 tggctgtctt gtaagcgtga ttttagcgca gcgggcggca gctcgtcggt aaagagcgca
     3601 gtgacctgtg ccacgttacc aatttcaacc gccgccgaag catgatactt agtgtgatcg
     3661 gcgaccagca gaatatttct cgcgtgcgcc atcatcgttt tcaccacgtt agcttcgttt
     3721 acatcaaact ccatcaacgc gccatcgctc tcaatcgccc caacgcttgt taccagataa
     3781 tcagcacgaa aatcagccac aaaggacgcc gctgaagggc caatgatccc gctattatga
     3841 gagcgcaacg taccgccggg caccatcact tcaaagcgcg ggttgtggta aagaatatgc
     3901 gccacacgca ggctgttggt gattatccgc aaatgattat ggttaagtaa cgcccgggca
     3961 acatgctcaa cagtcgtacc aatggtgata aatattgttg aaccatcagg aatatagtct
     4021 gccaccgctt cggcaatcgc tttttttttc tcggtttgcg aaacctcacg ctgctcgaac
     4081 gccgtattaa cgacgctgga agcccgaccc gcgccaccgt gatggcgcgt aatcaggcct
     4141 tgctcgctta atttacgaat atcccggcgg accgtctgcg tggagacatc cagcagattt
     4201 gccagctcat caatattcat atacccacgc tcggcgatca gcatgaggag ttggtcgtgc
     4261 cgcgggttac cggttagttc ggtaaggctc atgaaaattc ctcgaaaaac catcaccctg
     4321 gcattttata caaaaagtga caaaaaagat cgggtttgat cacagtcagg gatcccggct
     4381 cgtccaccgg gacgtgtgca ttttaacgcc gctacaccgc tggcgaagcg gactgactcc
     4441 gctaaatccc cacttgttgc cagcgccacc gccaaagcgc cgtgaaaaac atctcccgca
     4501 cctgtggtat ctaccacatc aactttgaag gccggttgat gctgacgccc accattttcc
     4561 agccagtcgc agcctgcgct accctgggtc acatagacat gaccatttgt gagcgtttgt
     4621 gcctgtttta gcgcactggc catctctttc acgcccgtta agcgcgccag acccggttct
     4681 gaaaaggccg cgtgatcgct taatgccacc agctcactga tatcctgcgg cgtaatgtcc
     4741 ccgtccagaa cggtcatcac acccgcctga cgggccaggg tgaaggcttt tttagcgccg
     4801 tcgtgccagc gtacatctgc cagcacaaca tcccactgag agaaatcaat ttcctccaac
     4861 cactctgcgt caggcagcag atccgggctg gggtagttaa tgattatccg ctcgcctttg
     4921 gtatccacca tgatggcgga ttgcgaagat ttcgcctggt tataccgttt ggtgtaacgg
     4981 gtgttaacgc cccaggattc cagctctgcc agcaggctgt tgcccgcgtc gtcatcacct
     5041 acgcgaccaa taaaatcgac ctgcgccccc agccttgccg ccgcaaccgc tgccgtcgcc
     5101 gctggcccgc caccaacttc cgtataattt ctcgccacgt atttaccgct ctccgtcggt
     5161 aacccttcca cgtaatagat gcgatccatc acggttatac ctacacaagc aacacgaatc
     5221 atggtcattc cttaaacatt ttgggatgcg ttcattttaa tttcaccaat tgtttaaaaa
     5281 gtgacggctg tcaatttttt gacgataaat tacaaattcg atcaaaaaca gacaagcaaa
     5341 gcatgtcata aatgacaaaa agtgacatca ctgtattcag gagagggtta tggcagcaat
     5401 cgcgtttatc ggtttaggac aaatgggttc gccaatggcg agcaatttat tgcagcaagg
     5461 gcatcaactt cgcgtctttg atgtgaacgc cgaagctgtg cggcatctgg tagacaaagg
     5521 tgcgactccc gccgccaacc cggcgcaggc cgctaaagat gccgaattta tcattaccat
     5581 gttgccgaat ggcgatctgg tgcgcaacgt gttgttcggt gaaaacggcg tttgcgaagg
     5641 cttatctacc gatgcgctgg tcattgatat gtccaccatc catccgctgc aaaccgataa
     5701 attgattgcc gatatgcaag ccaaaggctt cagcatgatg gatgttccgg taggccgtac
     5761 ttctgcaaat gccattaccg gtactctgtt actgctggct ggcggcaccg ctgaacaagt
     5821 tgaacgtgcc acaccgatcc tgatggcgat gggcagtgag ttgatcaacg ctggcggtcc
     5881 gggcatgggg atccgcgtta agctcatcaa caactacatg agcatcgcgc tcaatgcgct
     5941 ttcggcagaa gccgccgttt tgtgcgaagc cctgaatctt cccttcgatg ttgccgtcaa
     6001 agtgatgagc ggtaccgccg ccggtaaagg tcacttcacc acttcctggc caaacaaagt
     6061 cctcagcgga gatctttctc ccgccttcat gatcgatctt gcccataagg atcttggcat
     6121 cgcccttgat gtcgccaacc agctgcatgt gccaatgccg ctgggggccg cctcacggga
     6181 ggtttatagc caggcgcgcg cagcgggtcg cggtcgccag gactggtccg ccattctgga
     6241 acaggtccgt gtcagtgccg ggatgactgc caaagtaaaa atgtaacgac tggataaagg
     6301 aataaatgaa tgaataagta caccatcaac gacattacgc gcgcatcggg cggttttgcc
     6361 atgctggcgg tcgatcagcg cgaagccatg cgcatgatgt ttgccgcggc tggggcaccc
     6421 gctccggtag ccgatagcgt tttaactgat ttcaaagtta acgctgcaaa ggccctctcg
     6481 ccttatgcct cggcgattct ggtagatcaa caattctgct accgccaggt ggttgagcaa
     6541 aacgcgattg ccaaaagttg cgccatgatt gtcgccgccg atgagttcat tcctggcaac
     6601 ggtattccgg tcgatagcgt ggttattgac cgcaaaatca atccgctaca gatcaaacaa
     6661 gacggtggca aagccttaaa actgctggtg ctgtggcgta gtgatgaaga tgcgcagcaa
     6721 cgtctggata tggtgaaaga gttcaacgaa ctgtgccact cacacggtct ggtaagcatc
     6781 attgagccag tcgtccgccc accgcgtcgt ggcgataaat tcgatcgcga acaagcgatc
     6841 atcgatgccg ccaaagagct gggcgacagt ggcgctgacc tctacaaagt tgaaatgccg
     6901 ctttatggca aaggtccgca acaagagctt ctctgtgctt cacaacgtct gaatgaccat
     6961 atcaatatgc catgggtgat cctctcttcc agtgtcgacg aaaaactgtt cccgcgtgcc
     7021 gtacgcgtgg caatgacggc gggcgcatcg ggattcctgg caggccgtgc agtctgggca
     7081 tcggtcgtcg gtttaccaga caacgagctg atgctccgtg acgtttgcgc accgaaatta
     7141 caacaacttg gcgatatcgt cgacgaaatg atggctaaac gccgctaaga tgaggataaa
     7201 aggaatgaaa tggtttaaca ccctaagcca caaccgttgg ctggaacagg aaaccgaccg
     7261 catctttgat tttggtaaaa attccgtagt gccgactggt tttggctggt taggcaataa
     7321 agggcaaatc aaagaagaga tgggcaccca tctgtggatc acagctcgta tgttgcacgt
     7381 ttattccgtt gctgcggcga tgggtcgacc tggcgcttac gcgttggttg atcacggtat
     7441 taaagccatg aacggcgcac tgcgtgataa aaaatatggc ggctggtatg cctgcgtgaa
     7501 tgacgagggc gtggtggatg cctccaaaca gggctatcaa cacttctttg ctctgctggg
     7561 tgccgccagc gccgtcacaa cgggtcaccc ggaagcgcgc aagctgctcg attacaccat
     7621 tgagattatc gagaaatatt tctggagcga agaagagcag atgtgcctgg agtcctggga
     7681 cgaagccttc agcaaaaccg aagagtaccg cggcggcaat gccaatatgc acgcggtgga
     7741 agctttcttg attgtttatg acgtcactca cgacaaaaaa tggctggatc gcgcgattcg
     7801 cgtggcttcc gtgattatcc acgacgtcgc cagaaataat cattatcgcg ttaacgaaca
     7861 cttcgatacc cagtggaatc cgctgccgga ttacaacaaa gataacccgg cgcatcgctt
     7921 ccgcgcgttc ggaggtacac caggccactg gatcgaatgg ggccgtttaa tgctgcacat
     7981 ccatgctgcc ctggaagccc gttgcgaaca accaccagca tggctgctag aagatgccaa
     8041 aggtctgttt aacgccaccg tgcgcgatgc ctgggcaccc gatggagcgg acgggattgt
     8101 ttataccgtt gactgggaag gaaaaccggt ggtccgcgaa cgtgtacgtt ggcctatcgt
     8161 cgaagcaatg ggtacggtct acgcgctcta caccgtcact ggcgatcgcc agtatgaaac
     8221 ctggtatcaa acatggtggg agtactgcat taagtacctg atggactatg aaaatggttc
     8281 ctggtggcag gagctggatg cggacaataa ggtcatcacc aaagtctggg acggcaaaca
     8341 ggatatttat cacctgctgc attgcctggt gatcccgcgt atcccgttag cgcccggcat
     8401 ggctccagcg gttgcagcgg gtctgctgga tattaatgcg aaataactga tctgaaaata
     8461 gggtgcgggt tttcccctca ccctaaccct ctccccaaag gggcgagggg accgtccggt
     8521 gcagtttttg ggtttgacat cagcctcacg ggaggctgta tgtcgttaat taaggtgcct
     8581 tgtatgcaaa tcaccaatat gcattgcagt ggacagaccg tttcacttgc cgcgggggat
     8641 tatcacgcga ccattgtgac cgtcggtgct ggtctggctg aattaacctt tcagggttgt
     8701 catttagtca tcccgcacaa acccgaagag atgccgctgg cgcatctggg taaagtgctt
     8761 attccgtggc cgaaccggat cgccaacggc tgttaccgtt atcaggggca ggaatatcag
     8821 ttacccatta atgaacatag ttcaaaagcc gctattcacg ggctgcttgc ctggcgagac
     8881 tggcaaatta gtgaattaac caccaccagc gttaccctga cagcttttct gccgcccagt
     8941 tacggctatc cgtttatgct ggcttcacaa gttgtttatt cgctcaacgc acacacgggt
     9001 ctttccgttg agatcgcaag ccagaatatc ggcactgtcg cagcgcctta tggcgtgggt
     9061 attcatcctt atttaacctg caacttaaca tctgttgatg aatatctgtt ccagcttccg
     9121 gcaaaccagg tttatgccgt cgatgaacac gtaaatccga caacgcttca ccacgttgat
     9181 gagctggatt taaatttcac gcaggcgaaa aaaatagccg cgacaaaaat cgaccacacc
     9241 tttaaaaccg ctaatgattt gtgggaaatg accattaccc atccacagca ggcgctttcg
     9301 gtaagccttt gttccgatca accctgggtg caggtttata gcggtgaaaa attacagcgt
     9361 cagggactgg cagttgaacc tatgagttgc ccgcctaacg cctttaattc aggtatcgac
     9421 ttattattac tggaaccagg aaaaccgcac cgcctgtttt ttaatattta cggacagaga
     9481 aaataacttg tattgattag cagtcaaaat cattgaagaa catttcactc gctaaaggca
     9541 acattatgta tcttcaattt attaagttaa aaacagctca gcttctgtaa agtttttata
     9601 tatcagtttt atgagaatca ttttacataa gcctgtaata acaggaaggc aacaatctcg
     9661 ttctcaactc tatggagatt agttatggat acgccacgtc cacagttatt agattttcaa
     9721 tttcatcaga ataacgacag ttttacccta cattttcaac aacgtcttat tttaacccat
     9781 aacaaagata atccttgttt atggattggc tcaggtatag cggatatcga tatgttccgc
     9841 ggtaatttca gcattaaaga taaactacag gagaaaattg cgcttaccga cgccatcgtc
     9901 agccagtcac cggatggttg gttaattcat ttcagccgtg gttctgacat tagcgccacg
     9961 ctgaatatct ctgccgacga tcagggccgt ttattgctgg aactacaaaa cgacaacctt
    10021 aaccacaacc gtatctggct gcgccttgcc gctcaaccag aggaccatat ctacggctgc
    10081 ggcgaacagt tttcctactt cgatctgcgc ggcaaaccgt tcccgctatg gaccagtgaa
    10141 caaggcgttg gtcgcaacaa acaaacctat gtcacctggc aggccgactg caaagaaaat
    10201 gcgggcggcg actattactg gactttcttc ccacagccta cgtttgtcag cacgcagaag
    10261 tattactgcc atgttgataa cagttgctac atgaacttcg actttagcgc cccggaatac
    10321 catgaactgg cgctgtggga agacaaagct acgctacgtt ttgaatgtgc tgacacatac
    10381 atctccctgc tggaaaaatt aactgccctg ctgggacgcc agccagaact gcccgactgg
    10441 atttatgacg gggtaacgct cggcattcag ggcgggacgg aagtgtgcca gaagaaactg
    10501 gacaccatgc gtaacgcggg cgtgaaggtt aacggcatct gggcgcagga ctggtctggg
    10561 atccgtataa cctcttttgg caagcgcgtg atgtggaact ggaagtggaa cagcgaaaac
    10621 tacccgcaac tggattcacg cattaagcag tggtatcagg agggcgtgca gttcctggcc
    10681 tatatcaacc cgtatgttgc cagcgataaa gatctctgcg aagaagcggc acaacacggc
    10741 tatctagcaa aagatgcctc tggcggtgac tatctggtgg agtttggcga gttttacggc
    10801 ggcgttgtcg atctcactaa tccagaagcc tacgcctggt tcaaggaagt gatcaaaaag
    10861 aacatgattg aactcggctg cggcggctgg atggctgact tcggcgagta tctgcccacc
    10921 gacacgtacc tgcataacgg cgtcagtgcc gaaattatgc ataacgcctg gcctgcgctg
    10981 tgggcgaagt gtaactacga agcccttgaa gaaacgggca agctcggcga gatcctcttc
    11041 tttatgcgcg ccggttctac cggtagccag aaatactcca ccatgatgtg ggcgggcgac
    11101 cagaacgtgg actggagtct cgacgatggc ctggcgtccg tagtcccggc ggcgctgtcg
    11161 ctggcaatga ccggacatgg tctgcatcac agcgacattg gcggttacac caccctgttt
    11221 gagatgaagc gcagcaaaga gctgctgctg cgctggtgcg atttcagcgc cttcacgccg
    11281 atgatgcgca cccacgaagg taaccgtcct ggcgacaact ggcagtttga cggcgatgcg
    11341 gaaactatcg cccattttgc ccgtatgact accgttttca ccaccctgaa accttatctg
    11401 aaagaggccg tcgcgctgaa tgcgaagtcc ggtctgccgg tcatgcgccc gctgttcctg
    11461 cattacgaag acgatgcaca tacctattcc ctgaaatatc agtacctgtt aggccgcgac
    11521 attctggtcg ctccggtaca cgaagaaggt cgcagcgact ggacgctcta tctgccggag
    11581 gataactggg tacatgcctg gacgggtgaa gcgttccggg gcggggaagt taccgttaat
    11641 gcgcccatcg gcaagccgcc ggtcttttat cgcgccgata gcgaatgggc ggcactgttc
    11701 gcgtcgttaa acagcatcta atcacatccg cccgtgggga aactcatggg caaagggaga
    11761 acatcgatga gtcacatcac aacggaagat ccagcaactt tacgcctgcc ctttaaagag
    11821 aaactctctt acggtattgg cgacctggcc tctaacatcc tgctggatat cggtacgctt
    11881 tatcttttga agttttatac cgacgttctg gggctgccag gcacctatgg cggcattatc
    11941 tttttgattt caaaattctt tactgcgttt accgatatgg gtaccggcat tatgttggat
    12001 tcccgacgca agatcggtcc aaaaggtaag ttccgtcctt ttattctgta tgcgtcattc
    12061 ccggtcacct tactggcgat cgccaacttt gtcggcacac cgtttgatgt caccggtaaa
    12121 acggtgatgg ccactattct gtttatgctt tacggactgt ttttcagcat gatgaactgc
    12181 tcctacggcg caatggtccc ggcaatcacc aaaaacccca acgaacgcgc ctcactggcg
    12241 gcatggcgtc agggcggcgc tacgctgggc ctgctgctgt gcacggtggg attcgtgcca
    12301 gttatgaatc ttatcgaagg taatcagaaa cttggctata tcttcgccgc cacgctgttt
    12361 tcactgttcg gcctgctgtt tatgtggatc tgctactcag gcgtgaaaga gcgttatgtc
    12421 gaaacccaac caaccaatcc ggcgcaaaag cctggcctgt tgcaatcttt ccgcgcaatt
    12481 gccggtaacc gcccactgtt cattctgtgt attgccaacc tctgcacctt aggggcgttt
    12541 aacgtcaagc tcgccattca ggtctattac acccagtacg tacttaacga tcccatcctg
    12601 ttgtcgtata tgggattttt cagcatgggc tgtattttca tcggcgtgtt cctgatgcct
    12661 ggcgcagtca gacgttttgg taagaagaag gtctatatcg gcggcctgct gatttgggtg
    12721 ctgggcgatc tgctcaacta tttcttcggc ggcggctcgg tcagctttgt ggcgttctcc
    12781 tgcctggcgt tcttcggctc agcgcctgaa ctgggcgctg gtttccgaca ccgtcgagta
    12841 cggcgagtgg cgcaccggcg tgcgttcgga aggcacggtc tacaccggct tcaccttctt
    12901 tcgcaaagtg tctcaggcgc tggctggttt cttccccggc tggatgctga cgcaaattgg
    12961 ctatgtgccg aacgtcgcac aggctgacca caccattgaa gggttacgcc agttgatctt
    13021 catctaccca agcgcactgg cggtagtcac cattgtggcg atgggttgct tctacagcct
    13081 gaacgagaag atgtatgtcc gcattgtgga agagatagaa gcccgtaaac gcacggcgta
    13141 ataataataa acgacgccct gcggggcgtt ataaggagtg attatgtctg accataatcc
    13201 actgacatta aaactgaatc tgcgggaaaa aatcgcctat ggtatgggcg acgtcggttc
    13261 gaatttaatg ctctgcatcg gtactctgta tctcctcaaa ctttataccg atgaattagg
    13321 gatgcctgct tactatggcg ggattatctt tctggtcgcg aagtttttta ccgcgtttac
    13381 cgatatgctc accggatttt tactcgactc gcggaaaaat attgggccta aaggcaaatt
    13441 tcgtcccttt attttatatg cagcagttcc ggcggcgtta attgcaacgc tccagtttat
    13501 cgccaccacc ttttgtttgc cggttaaaac gacgattgcc accacgctgt ttatgatgtt
    13561 tggactttca tacagtctga tgaactgctc gtatggtgcg atgatcccgg caattaccaa
    13621 aaacccgaat gagcgcgcgc agctcgcggc ttaccgccag ggtggtgcaa ccatagggct
    13681 attgatttgt accgtggcgt ttattccgtt gcagtcgctt ttttctgact caaccgtcgg
    13741 ttatgcctgt gcggcactta tgttctccat tggcggcttt atttttatga tgctgtgcta
    13801 cagaggcgtc aaagagcatt atgtggacac agcgccaacc ggacataaag ccagtattct
    13861 caaatctttt tgcgcgatat ttcgtaatcc accattgctg gttttatgca ttgctaacct
    13921 gtgtactctg gcggcattta atatcaaact ggcgattcag gtctattaca cccagtatgt
    13981 gctgaatgat attaatttat tgtcgtggat gggattcttc agtatgggat gcatcctcgt
    14041 cggcgtatta ctggtgccag taactgtaaa atgttttggt aaaaaacagg tttatttagc
    14101 tggcatggtg ctatgggcgg tgggtgatat actgaattat ttctggggaa gtaactcttt
    14161 cactttcgtc atgttctctt gtgtcgcctt ttttggcacg gcctttgtaa acagcctgaa
    14221 ctgggcactg gtaccagata ccgtagatta cggtgaatgg aaaaccggta ttcgtgccga
    14281 aggttctgtt tataccggtt ataccttctt tcgtaaaatt tctgccgcac ttgctggctt
    14341 cttgccaggc attatgctga cgcaaattgg ttatgttccc aacatcgcgc aaagcgatgc
    14401 gacattacag ggtttgcgtc agctcatttt tatctggcct tgtgcactgg caattattgc
    14461 tgcattgacg atgggattct tttacacact caatgaaaaa cggtttgcat tgattattga
    14521 ggaaatcaac caacgcaaaa ataaagagat ggcgacagaa aaaaaaacgg cttccgtaac
    14581 gttataaatg tcaatagccg ctatttccat cctggtgggt ggcggcctcc ctacgtttaa
    14641 aaaatggact tattatgaaa aagattaatg caataatttt attatcttct ctgacctctg
    14701 cctcggtatt tgctggcgca tacgtcgaaa acagggaggc atataatctt gcctccgatc
    14761 agggcgaggt tatgctacgt gtgggttata acttcgatat gggcgcgggt attatgttaa
    14821 ccaataccta cacctttcag cgagaagatg aactaaaaca tggctataat gaaattgaag
    14881 gttggtatcc tttattcaaa ccaaccgata aattaaccat ccagcccggt ggcttaatta
    14941 atgataagag tatcagttcg ggtggtgcag tgtatctgga
//
LOCUS       DAJTSI010000098        14822 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000098 DAJTSI010000000
VERSION     DAJTSI010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14822
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.098"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            256..780
                     /locus_tag="OO327_002739"
     CDS             256..780
                     /locus_tag="OO327_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6019222.1"
                     /translation="MKRSIIAAAVFPSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            861..1535
                     /locus_tag="OO327_002740"
     CDS             861..1535
                     /locus_tag="OO327_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6019223.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            1551..4013
                     /locus_tag="OO327_002741"
     CDS             1551..4013
                     /locus_tag="OO327_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6019224.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGWNTRIGA
                     ISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVW
                     ANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGR
                     SGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDVTTPRRQ
                     IYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPV
                     ATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQK
                     YRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTD
                     QRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQ
                     QGLSCTITFGKEIDESRNYICQ"
     gene            4029..5062
                     /locus_tag="OO327_002742"
                     /pseudo
     CDS             4029..5062
                     /locus_tag="OO327_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(5144..5482)
                     /gene="rcnB"
                     /locus_tag="OO327_002743"
     CDS             complement(5144..5482)
                     /gene="rcnB"
                     /locus_tag="OO327_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCR6019225.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5701..6531)
                     /gene="rcnA"
                     /locus_tag="OO327_002744"
                     /pseudo
     CDS             complement(5701..6531)
                     /gene="rcnA"
                     /locus_tag="OO327_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            6653..6925
                     /gene="rcnR"
                     /locus_tag="OO327_002745"
     CDS             6653..6925
                     /gene="rcnR"
                     /locus_tag="OO327_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCR6019226.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      7017..7113
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7148..7936
                     /gene="thiM"
                     /locus_tag="OO327_002746"
     CDS             7148..7936
                     /gene="thiM"
                     /locus_tag="OO327_002746"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR6019227.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7933..8733
                     /gene="thiD"
                     /locus_tag="OO327_002747"
     CDS             7933..8733
                     /gene="thiD"
                     /locus_tag="OO327_002747"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR6019228.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8798..9619
                     /locus_tag="OO327_002748"
     CDS             8798..9619
                     /locus_tag="OO327_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR6019229.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            9667..10413
                     /locus_tag="OO327_002749"
     CDS             9667..10413
                     /locus_tag="OO327_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6019230.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAAEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10387..11352)
                     /locus_tag="OO327_002750"
     CDS             complement(10387..11352)
                     /locus_tag="OO327_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HCR6019231.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11349..12352)
                     /locus_tag="OO327_002751"
                     /pseudo
     CDS             complement(11349..12352)
                     /locus_tag="OO327_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            12392..13444
                     /gene="fbaB"
                     /locus_tag="OO327_002752"
     CDS             12392..13444
                     /gene="fbaB"
                     /locus_tag="OO327_002752"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR6019232.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13752..14606
                     /gene="gatY"
                     /locus_tag="OO327_002753"
     CDS             13752..14606
                     /gene="gatY"
                     /locus_tag="OO327_002753"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR6019233.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14635..>14822
                     /locus_tag="OO327_002754"
     CDS             14635..>14822
                     /locus_tag="OO327_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HCR6019234.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPA"
BASE COUNT         3949 a         3411 c         3710 g         3752 t
ORIGIN      
        1 aatcatttat ataaatgatt tcgtcttttt tattgatatc aacaatacca tttacataaa
       61 tctacattaa ttaatagatg tgataaatat ttaatattct taagtatttg ttgtatttat
      121 attgcattgg ttaatttgtt ttaaatcaat aaaacaatat gtttacattg cggcagcttt
      181 tttgatgtga gaaatttgtt tccaagagat acagactctt aacaaccaaa aataaatttt
      241 attaaggaac atattatgaa acgttcaatt attgctgccg ctgtctttcc ttcttttttt
      301 atgagcgctg gagtatttgc tgcagacgtt gataccggaa cattaaccat taaaggaaat
      361 attgcagaat ctccgtgtaa attcgaagcg ggtggtgatt cagtaagtat taatatgccg
      421 actgtaccaa ccactgtctt tgaaggtaaa gctaaatatt ctacctatga tgatgcagtc
      481 ggtgtaacca gcagcatgtt aaaaattagc tgcccgaaag aagttgctgg tgtaaaactc
      541 tcgttgatca caaacgacaa aataaccggt aacgataagg cgatagccag tagcaacgat
      601 accgtaggtg ataacagcga tgtcctagat gtttctgcac cttttaacat tgagagttat
      661 aaaacagcgg aaggtcaata tgctattccg tttaaagcaa aatacctgaa actgacagat
      721 aactcagtgc aatcaggtga tgtgttatct tctctggtta tgcgtgtggc gcaggattaa
      781 taaataaaat tgcaagtttc tccaggtaat aaacatggcc gctatcccca tggcggcttt
      841 ttaatttaag aggcattaag atgaaaggat tattatcttt actcattttt tctatggtcc
      901 ttcctgcaca tgccggaatt gttatctacg ggacgcgcat tatttacccg gcagaaaata
      961 aagaagttat ggtgcagttg atgaaccagg gaaatcgttc ttcgctgctg caggcgtgga
     1021 ttgatgatgg cgatacgtcg ttaccaccag aaaaaattca ggttcctttc atgttaacgc
     1081 caccagtggc aaaaataggg gcaaattccg ggcagcaagt aaaaatcaaa attatgccga
     1141 ataaactgcc cactaataaa gaaagcattt tttatctgaa tgttctgtat attccaccaa
     1201 atagtccaga gcaagaaggc aagaatgcac tgaagtttgc gatgcaaaac agaattaagt
     1261 tgttttaccg gccagcgggt attgctccgg taaataaagc gacctttaaa aagttactgg
     1321 taaatcgcag tggcaatgga ttggtgataa aaaatgactc agctaattgg gtgacgattt
     1381 cggatgtcaa agctaataat gttaaagtca attatgaaac tattatgatt gcccccttag
     1441 aaagtcagag tgttaatatc aaaagtaata atgcaaataa ctggtatctg accattatca
     1501 atgaccacgg caactatatt agtgacaaaa tttaattgca ggagctgcct atgttgagaa
     1561 tgaccccact tgcatcagca atcgtagcgt tattgctcgg cattgaagct tatgcagctg
     1621 aagaaacctt tgatacccat tttatgatag gtggaatgaa agaccagcag gttgcaaata
     1681 ttcgtcttga tgataatcaa cccttaccgg ggcagtatga catcgatatt tatgtcaata
     1741 agcaatggcg cgggaaatat gagattattg ttaaagacaa cccgcaagaa acatgtttat
     1801 caagagaagt tatcaagcgg ttaggcatta atagcgataa cttcgccagc ggtaagcaat
     1861 gtttaacatt tgagcaactt gttcagggtg ggagctatag ctgggatatc ggggtttttc
     1921 gtctcgattt cagtgtcccg caggcttggg tggaagaact ggaaagtggc tatgttccac
     1981 cggaaaactg ggagcggggt attaatgcgt tttatacttc ttattatgtg agtcagtatt
     2041 acagcgacta taaagcgtcg ggtaacaaca agagtacata tgtacgtttt aacagcgggt
     2101 taaatttact ggagtggcaa ctgcattctg atgccagttt cagtaaaaca aataacaatc
     2161 caggggtgtg gaaaagcaat accctgtatc tggaacgtgg atttgcccaa tttctcggca
     2221 cgcttcgcgt gggtgatatg tacacatcaa gcgatatttt tgattctgtt cgcttcagcg
     2281 gtgtgcggtt gtttcgtgat atgcagatgt tgcctaactc gaaacaaaat tttacgccac
     2341 gggtgcaggg gattgctcag agtaacgcgc tggtaactat tgaacagaat ggttttgtgg
     2401 tttatcagaa agaggttcct cctggcccgt ttgcgattac agatttgcag ttggccggtg
     2461 gtggagcaga tcttgatgtt agcgtgaaag aggcggacgg ctcggtaacc acctatctgg
     2521 tgccttatgc agcggtgcca aatatgctgc aacccggcgt gtcgaaatat gattttgcgg
     2581 cgggggcgag caagcaaagt gattttgtcc aggcaggtta tcagtatggt tttaataact
     2641 tattgacgct gtatggcggc acgatggttg ctaataatta ctatgcgttt accctcggaa
     2701 cgggttggaa cacgcgcatt ggcgcaattt ccgtcgatgc cacgaagtcg catagtaaac
     2761 aagacaacgg cgatgtgttt gacgggcaaa gttatcaaat tgcctacaac aaatttgtga
     2821 gccaaacgtc gacgcgtttt ggtctggcgg cctggcgtta ttcgtcgcgt gattaccgga
     2881 catttaacga tcacgtttgg gcaaacaata aagataatta tcgccgtgat gaaaacgata
     2941 tctatgacat tgccgattat taccagaacg attttggccg taaaaatagc ttttctgcca
     3001 atatgagcca gtcattgcca gaaggctggg gttctgtgtc cttaagtacg ttatggcgag
     3061 attactgggg gcgtagcggc agcagtaagg attatcagtt gagttattcc aataactggc
     3121 ggcggataag ctataccctc gcggcaagcc aggcgtatgg cgagaatcat catgaagaga
     3181 aacgttttaa tatttttata tcaattccct gtgattgggg tgatgacgtt acgacgcctc
     3241 gtcggcaaat atatatgtct aactcaacga cgtttgatga tcagggtttt gcctcaaata
     3301 atacgggatt atcaggaaca gtagggagtc gggatcagtt caattatggt gtcaacctga
     3361 gtcatcaaca tcagggaaat gaaacgacag ctggggcgaa tttgacctgg aacgcgccgg
     3421 ttgcgacagt gaatggcagt tatagtcagt cgagtactta tcgacagact ggagccagtg
     3481 tttcaggggg cattgtcgcc tggtcgggtg gcgttaatct ggcgaaccgt ctttccgaaa
     3541 cgtttgctgt gatgaatgcg ccaggaatta aagatgctta tgtcaatggg caaaaatatc
     3601 gcacaacaaa ccgtaatgga gtggtggtat acgacggaat gacaccttat cgggaaaatc
     3661 acctgatgct ggatgtgtcg caaagcgata gcgaagcaga attacgtggc aaccggaaaa
     3721 ttgccgcccc ttatcgcggc gcggttgtac tggttaattt tgataccgat cagcgcaagc
     3781 cctggtttat aaaagcgtta agagcggatg gtcaaccatt aacgtttggt tatgaagtca
     3841 atgatatcca tggtcataat attggcgttg tcggccaggg aagccagtta tttattcgca
     3901 ccaatgaaat accgccatcg gttaatgtag caattgataa gcaacaagga ctttcatgca
     3961 caatcacctt cggtaaagag attgatgaaa gtagaaatta tatttgccag taatagtcag
     4021 gtggttctat ggaaattcgc ataatgctat ttatattaat gatgatggtt atgcctgtga
     4081 gctatgcggc atgttatagt gagttatctg ttcagcacaa tttggttgtt caggggaatt
     4141 ttgcacttac tcaaacacaa atagcgacat atgagcataa ttttaatgat tcgtcatgcg
     4201 tgagtacaaa tactatcacc cctatgagcc catcggatat tattgttgga ctttataacg
     4261 ataccattaa attaaattta cattttgaat ggaccaataa aaacaacatc acgttgtcaa
     4321 ataatcagag cagtttcacc agtggttatt cggtaacggt gacacctgcg gccagtaatg
     4381 caaaagtgaa tatttctgcg gggggcggtg gttcagtgat gattaatggt gttgcgacat
     4441 tatccagtgc ttcatcatcg acacgcggga gtgccgcagt acaatttcta ctgtgtttat
     4501 taggtggcaa gtcatggggt gcatgtgtaa atagctacag aaatgcattg gcacaaaatg
     4561 caggtgtcta ttccttcaat ctgacattgt catacaaccc gataaccaca acctgcaaac
     4621 aggacgattt attaattact ttagacagta ttcccgtttt acaattacca gccacaggta
     4681 acaaagcaac aataaatagt aaaaaaaggg atattattct gcgttgtaaa aatttattag
     4741 gtcaacaaaa tcaaacatca cggaaaatgc aggtgtattt atcaagttct gacttgttaa
     4801 ccaacagcaa cacaatactg aaaggtgcgg aagataatgc gtaggattta ttcttgaaag
     4861 taatggttcg ccagtcacac ttttaaatat cactaacagc agtaaaggat acacaaattt
     4921 aaaagaaatt gcggcaaagt caaaacttac agatacaacg gtttcaattc cgataacagc
     4981 cagttactac gtctacgata caaacaaagt taaatctggc gcactggagg caaccgcatt
     5041 aatcaacgtg aaatacgact aataacaagc aaaacgagca agtggctaat aataaaaaat
     5101 aacaaggtgt ctgcatttcc ccctcatgat gagggggctt tttttagcga tgataaaaaa
     5161 tctcaccgtc gtaggcttta atgattttac cgtcggtgtc gctgatcaac acgtacgcgc
     5221 cacccatata ggtccagtgc gtcccggcat caggcgcggg caggttacgt aactgccact
     5281 gcttaatgtt gtactcatcg gtgcggtaca tctctgggac ggtatcacct ggtttgaaat
     5341 gcttaaagtc agcaatgaaa ctacttaact catacttaga aattcccgag gtattggtcg
     5401 aacccgcggt ggctggtgcg gcccaggcgg cactggtaac cagcaaaagc acacccagca
     5461 acatcttatt tttaatggtc ataaactctc cactttgggg atctgtaata tcagtcgcat
     5521 atctttcctg tcatacaata taactgacaa gcgatatttt gttagtgatc ccatgatttc
     5581 gtaaaaagtg taaatgaaag gatgccctaa aagcatcctt agcgagcaga gagtcagcgg
     5641 aaatagctga tttaagaatc atcctgggct gaagataaca tgcaagccta aaggattgag
     5701 ttatcgcatt atgcccatga agccatgtac gcccatatac acaccgacta agccaatcaa
     5761 cagactggaa aaataggggg cgcgtttagc gagagtgtta aatccgctcc agcgttttgc
     5821 gacctgctga acgctgattg ctgcgccaac gcctacggtg acaagcgtta acgccaggcc
     5881 aatgctgaaa ctgacgacca gtgttgcgcc cagtgtcagg gctttcaact gaatgcatat
     5941 caacagcacg gtaattgctg ccgggcaggg gataaagcca ccggttaagc caaataacaa
     6001 aatttgccag ttgatgacct ctctaccatc aaagcgtcgt ttaatgtcat tggcatgggc
     6061 tcgtgcatgg gcatcctgat actcgccatg ttcgtgatgg tgatgatgtc cttggtcgtg
     6121 gtgatgttcg tgatcgtgat gatggtgatg atgctcataa tcatgcccgt gcatattctc
     6181 cagccagttg cgttcgccgc gctaggtacg ccagaacatc cagaacgcgg tgccaataat
     6241 gatcactgcg gaaatcagct ggagccacgg ttctgctgat tgagcagtaa agcgcttact
     6301 gatcaccatc ccgccaaagg caatcaacca gaccaccgcg gtatgcgaaa tggttgctgc
     6361 cagtccgagc atcaccgcct gtttaatggt gcctttgatg gcgatgataa acgccgccat
     6421 catcgttttt gagtgccctg gttccaggcc atgaagcgca ccaagtaaga tggcgctggg
     6481 gatgaagaac caggcgtttc cttgctgaag aagagttgta aattcggtca tgataataat
     6541 tcttagtatt aattcggcaa tctgattcta ctccccccca gtacctgata ctaccccccc
     6601 agtagattaa tagtgctatg atttttcata ttcttgttaa ccaggtgttg ccatgtctca
     6661 tacaatccgt gataaacaga aactgaaagc gcgtgccagt aagattcagg gccaggtcgt
     6721 ggcgctcaag aaaatgctcg acgagccgca cgaatgcgct gcagttttac aacagattgc
     6781 tgctatccgt ggagcggtaa acggtctgat gcgggaagtg attaaaggtc atctgacgga
     6841 acacatcgtt caccaggggg atgagctaaa acgtgaagaa gatctggatg tcgttctgaa
     6901 ggtgctggat tcatatatca aataatttat taacgcgatt gtaaaactgc cgtttttcct
     6961 cgtttacaac gggggcgctg gacattacca tcctcctctg cgatttatca tcgcaaccaa
     7021 acgactcggg gtgcccttct gcgtgaaggc tgagaaatac ccgtatcacc tgatctggat
     7081 aatgccagcg tagggaagtc acggaccacc aggtcattgc ttcttcacgt tatggcagga
     7141 gcaaactatg caagtcgacc tgctgagttc agcgcaatct gcgcacgcgt tacacctttt
     7201 tcaccaacat tcccctcttg tgcactgcat gaccaatgat gtggtgcaaa cctttaccgc
     7261 caataccttg ctggcgctcg gtgcatcgcc agcgatggtt atcgaaaccg aagaggccag
     7321 tcagtttgcg gctatcgcca gtgccttgtt gattaacgtt ggcacactga cgcagccacg
     7381 cgcacaggcg atgcgtgctg ccgttgagca agcaaaaagc tctcaaacac gctggacgct
     7441 tgatccagta gcggttggtg cgctcgatta tcgccgccat ttttgtcatg aacttttatc
     7501 ttttaaaccg gcagcgatac gtggtaatgc ttcggaaatc atggcattag ctggcattgc
     7561 taatggcgga cggggagtgg ataccactga cgccgcagct aacgcgatac ccgctgcaca
     7621 aacactggca cgggaaactg gcgcaatcgt cgtggtcact ggcgaggtgg attatgttac
     7681 cgatggacat cgtatcattg gtattcacgg tggcgatccg ttaatgacca aagtggtagg
     7741 aactggctgt gcattatcgg cggttgtcgc tgcctgctgt gcgttaccag gcgatacgct
     7801 ggaaaatgtc gcatctgcct gtcactggat gaaacaagcc ggagaacgcg cagtcgccag
     7861 aagcgagggg ccaggcagtt ttgttccaca tttccttgat gcgctctggc aattgacgca
     7921 ggaggtgcag gcatgaaacg aattaacgct ctgacgattg ccggtactga tccgagtggt
     7981 ggtgcgggga ttcaggccga tcttaaaacc ttctcggcac ttggcgctta tggttgctca
     8041 gttattactg cactggtggc gcaaaatacc cgtggcgtac agtcggtgta tcgcattgag
     8101 cctgattttg tcgccgccca gctcgattcg gtgttcagcg atgtgcgaat cgataccact
     8161 aaaatcggta tgttggcgga aaccgatatt gttgaagcgg tggcagaacg gttgcaacgt
     8221 tatcagatcc aaaacgtggt actcgacacc gttatgctgg caaaaagcgg cgacccgctg
     8281 ctttcacctt cggcggttgc tacgctgcgc agtcgattat tgccacaggt ttcattaata
     8341 acgccaaact tgcccgaagc tgccgccttg ctcgacgcgc cacacgcgcg caccgaacag
     8401 gaaatgctgg aacaagggcg atcgctgttg gcgatgggct gtggcgcagt gctaatgaaa
     8461 ggtggtcatc tggatgatga gcaaagcccg gactggctgt ttacccgcga gggtgaacaa
     8521 cggtttaccg caccgcgcat tatgaccaaa aacacccacg gcactggttg tacactctct
     8581 gcggcgttgg ctgcactacg cccgcgccat acaaactggg ctgacaccgt acaggaggca
     8641 aaaatctggc tttcatcggc gttagcccag gccgacacgc tggaagttgg tcacggtatt
     8701 ggtccggttc accacttcca cgcctggtgg tgagcaccgt actatagctg ctttggttat
     8761 tgtcagcctt tggcactgac attcagggat gaaaccaatg cagctaagaa ttaccagtcg
     8821 aaaaaaactc accgctctat tatgcgctct tgggctaatt tccatcgttg caatttatcc
     8881 gcgtcagacg gtgaattttt tctattcgac agcagttcag attacagact acatccactt
     8941 ctacggttat cgcccggtta aatctttcgc tattcgcatt cctgccagtt acaccattca
     9001 cggattagat gtttcgcgct ggcaggagcg tatcgactgg cagcgggtgg caaaaatgcg
     9061 cgacaacgat atccgcttac agtttgcttt tattaaggct acggaaggcg aaaagctggt
     9121 ggacccctat ttttcgcgta actggcaatt aagccgcgaa aatggcctgc tgcgcggggc
     9181 gtatcattat ttctccccgt cggtatccgc ttcagttcag gcgcgattat ttctgcaaac
     9241 ggtggatttc tcacaaggcg atttccctgc cgtgctggac gtagaagaac ggggaaaatt
     9301 atcggcaaaa gaattacgca agcgggtaag tcagtggcta aaaatggtcg aaaaacgcac
     9361 gggaaaaaag ccgattattt actcaggagc cgttttttat cacaccaatc tggcgggcta
     9421 tttcaatgaa tatccgtggt gggtggctca ctattatcaa cgtcgtccgg acaatgacgg
     9481 catggcctgg cgcttctggc agcattccga ccgtggacag gtagatggca tcaatggtcc
     9541 ggtggatttt aatgtgttta atggcacggg gatgagttgc aggcattcgt tgatgggatt
     9601 aaagaaacgc cttaaataag aagcagtgat atactcccac cagactccct ggcaggaaaa
     9661 agacaaatgg aacaagcgca tacccagctg attgcccaac tgaacgagcg tattttagcg
     9721 gcggataaca cgccgcttta tattaagttt gccgaaacgg taaaaaatgc cgtgcgcagc
     9781 ggggtgctgg agcatggcaa tattttgccc ggtgagcgtg atttaagtca gttaaccggc
     9841 gtttcgcgca ttacggtgcg caaggcgatg caggcgctgg aagaagaggg cgtggtgacg
     9901 cgttcgcgcg gttacggcac gcagatcaac aacatcttcg aatactcgtt gaaagaagcg
     9961 agaggttttt ctcagcaggt ggtattgcgc gggaaaaagc ccgatacgct atgggttaat
    10021 aagcgtgtcg tgaaatgtcc cgaagaagtt gcgcagcagc tggcggccga agcgggaagt
    10081 gatgtctttt tgcttaagcg tattcgctat gtcgatgaag aagcggtatc gattgaggaa
    10141 tcgtgggttc cggcgcattt aattcatgat gttgatgcca tcggaatttc tctttatgac
    10201 tatttccgca gccagcacat ttacccacaa cgtacgcgtt cccgcgttag cgcccggatg
    10261 ccggatgccg agtttcagtc acatattcag ttagatagca aaataccggt gctggtgatc
    10321 aagcaagtgg cgcttgacca acagcaacgg cctattgagt acagcattag ccactgtcgc
    10381 agcgatctat acgtttttgt gtgcgaggag tagttcctcg cgcgttggcg cacaatcacc
    10441 gccccgatgc ccgacaaccc acgacgccac tgcattgccc agtaatacgg catccgccag
    10501 tggcagacca gaggccagcc cggcaagtac gccacctgca tgactgtcgc ccgccccaat
    10561 ggtgtctaca acttgcgttg gaaatgccgg aatgcagcca gaagcgtcgt tgctgaaata
    10621 ccatgcgcct tctttatcga gacgaacgat caacggcgcg gcaaatttct cctgccattg
    10681 cttgccaagt gttgttatct ctgcggataa agcaaaacgt tcggcggcaa tctcagcctc
    10741 ttgacgattg agtgacacta aaggtcgaca ggccatgatc cgcgccagta atgcgtccgg
    10801 gatatcgcca atacgtgggc aaaaatcgat aaacggcgtc acgtcttgca gcttttccag
    10861 ccattccact aacaattcgc cgcagggcga ggccagttga taaccggaaa aatagagcag
    10921 gttgccaggc gcaacggtta atcgtgccaa ccattggcga ttccattgat tttccacacc
    10981 gctgaatgac ataaaagtgc gttcgccatc cggctcaacc agtgccagac accagccgtt
    11041 atcgctttcg gcgttatcga tcaggctgat taacccctct ttcgccatcc ggttgcgaat
    11101 aatctccgcc cacacgccct gaccgagcgg caaggcatta cccgcttcga tgccgaggcg
    11161 ctttaacgcc acggcaatat tcagtgcgca gccgccaacg ttaacgctct gctgtttcag
    11221 ttcgatatcg cacccacgcc aggggagggc ataagcgtcg gcgatcacgt caatgaccgc
    11281 cgcgccgacg accatcaccg gctgacgcgt ggttaattca ggcagcagtg tgtgtaatcg
    11341 agcgccgctc ataccgcctc ccgttgttga cgatatttcg ccagcgctgt ggcatagcgg
    11401 ttgaaatcaa gctgatttac cgcatccagc tccgccttta acgcaggatc gacagcgtta
    11461 atgccatgca acgcgccgca aatcgccgtc gccatagcac ctatggtgtc tgtgtcgccg
    11521 ccaagattgg cgcacaggac ggcgcagcgg tttgggtcgg tttgtgccag ttcaactagc
    11581 gcaatggcgc acggaacgga ctcaatagtg ctggtgcctg cgccaacgac ctggtaaagc
    11641 tgttcgctgg cggattcggt gccgtcggca ttacgcacaa ttttcagcgc aatctccaga
    11701 cgtgccgcca gtgaggcgct gaacgtggtg gtgcgttttt gctgtgcatg tcgcgcaatt
    11761 gaaggcaatg aatcaacaat cgctgaccag cgttctccgt caatggcacg agaaatcgcc
    11821 catgcgatga ctaccgcgcc tgcaaccgcc agatcggatt tatgggtcgg gctggacgcc
    11881 agcgccacat catcaataaa ggaatcaaca tcacgcgctg gaagcaaaca acctaatggc
    11941 gagacgcgca tcgctgcgcc gttggttacg ccgttgtttt ccagttcagc aacgggttta
    12001 ccgtcgcgaa tggcgttaag cgcaatcttc gaggtcggac ctagtacgtt tttgttaaag
    12061 gcgtcgaaac gcaacgccca gtcgagaata ttacgcccaa tcagattcgg atcgatcttg
    12121 ccttcacatt ccagtaacgc atccgccaga cacaggccat cgaggtatcg tcggtgaatt
    12181 cggcgcggtt aaaataacag gccgcgttat tctcctttgg tccaggcaga aaacggtcaa
    12241 tccagccaaa gtgtgcttta acgcggctgc gtggccaaag ctcggagggc atccccatcg
    12301 catcccctaa cgcctgccca taaagagcac cgagaatacg ttctgttttc atttaacttc
    12361 cccttgaatc ggaatttacg ggagcatagt aatgacagat attgcgcagt tgcttggcaa
    12421 agacgccgac aaccttttac agcaccgttg tatgactatt ccttctgacc agctttatct
    12481 ccccggacat gactacgtag accgcgtgat gattgacaat aatcgcccgc cagcggtgtt
    12541 acgtaatatg cagacgttgt acaacaccgg gcgtctggct ggcacaggat atctttctat
    12601 tctgccggtt gaccagggcg ttgagcactc tgccggagct tcatttgctg ctaacccgct
    12661 ctactttgac ccgaaaaaca ttgttgaact ggcgatcgaa gcgggctgta actgtgtggc
    12721 atcaacttac ggcgtgttgg tgtcggtatc gcggcgctat gcgcatcgca ttccatttct
    12781 cgtcaaactt aatcacaacg agacgctaag ttacccgaat acctacgatc aaacgctgta
    12841 tgccagcgtg gagcaggcct tcaacatggg cgcggtggcg gttggtgcga ctatctattt
    12901 tggctcggaa gagtcacgtc gccagattga agaaatttct gcggcctttg aacgcgcgca
    12961 cgagctgggc atggtgacag tgctgtgggc ctatttgcga aactccgcct ttaagaaaga
    13021 tggcgttgat taccatgttt ccgccgacct gactggtcag gcgaaccatc tggcggcaac
    13081 cataggtgca gatatcgtca aacaaaaaat ggcggaaaat aacggcggct ataaagcgat
    13141 taattacggt tataccgacg atcgcgtgta cagcaagtta accagcgaaa acccgattga
    13201 tctggtgcgt tatcagttag ctaactgcta tatgggccgg gctgggttga taaactccgg
    13261 cggtgctgca ggcggtgaaa ctgacctcag cgatgcagtg cgtactgcgg ttatcaacaa
    13321 acgcgcaggc ggaatggggc tgattcttgg acgtaaggcg ttcaagaaat cgatggctga
    13381 cggcgtgaaa ctgattaatg ccgtgcagga cgtttatctc gatagcaaaa ttactattgc
    13441 ctgatacgcc tcatacgaac agcaccaccc cgtaggacgg ataagacgtt tacactgcat
    13501 ccggcaacct atgcctgatg cgacgcggaa gcgtcttatc atgcctacat agcactgcca
    13561 cgtatgttta caccgcatcc ggcataaaaa cacgcgcact ttgctacggc ttccctatcg
    13621 ggaggccgtt tttttgcctt tcactcctcg aacaattttc atattgttgt ttttgcgatc
    13681 attatctcga catttaaaag caaataattt cattatattt tgaaatcgaa agcaaacgac
    13741 aagatatgaa aatgtacgtg gtatcgacaa agcagatgct gaacaacgca cagcgcggcg
    13801 gttatgcggt tccggcattc aatattcaca atctcgaaac gatgcaagtg gtggtggaaa
    13861 ccgctgccaa cctgcatgcg ccggtcatca tcgccggaac gcctggcaca tttactcatg
    13921 ctggtacaga aaatctgttg gcgctggtca gcgcgatggc gaagcaatat caccatccac
    13981 tggcaattca tctcgaccat cacacgaaat ttgacgatat cgctcagaag gttcgttctg
    14041 gcgtgcgctc agtcatgatt gacgcctcgc atttgccttt tgcgcaaaat atttcacggg
    14101 tcaaagaggt ggtggatttt tgccatcgct ttgatgtcag cgtcgaggcg gagctggggc
    14161 aacttggcgg ccaggaagat gatgtgcaag tcaatgaagt cgatgcgttg tacaccaacc
    14221 ccgcacaggc gcgtgaattt gccgaggcaa ccgggattga ttccctggcg gtcgccatcg
    14281 gcacggctca cggtatgtat gccagcgcac cggtgcttga tttttctcga ctggagaaca
    14341 ttcgccagtg ggtgaactta ccgctggtgc tgcatggcgc gtcaggatta tcgactaagg
    14401 atattcagca aaccatcaaa ctggggatat gcaaaatcaa cgttgcaacg gagctgaaaa
    14461 atgccttctt gcagtcgtta aaaaattacc tgaccgagca ccccgaagcg accgatcccc
    14521 gggattattt gcagtcggct aaatccgcaa tgcgcgatgt ggtgagcaaa gtgattgccg
    14581 attgtggctg cgagggcagg gcataacgca cctgccattt aacaaggaaa aaacatgaaa
    14641 acgttaattg cccggcataa agctggtgaa catatcggca tatgttcagt ctgttctgcc
    14701 catccgttgg ttatcgaagc ggcgctggca tttgatcgca acagcacgcg caaagtgctg
    14761 attgaagcaa cgtcaaacca ggtcaatcaa tttggcggtt ataccggaat gacaccggca
    14821 ga
//
LOCUS       DAJTSI010000099        14479 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000099 DAJTSI010000000
VERSION     DAJTSI010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.099"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(113..379)
                     /locus_tag="OO327_002755"
     CDS             complement(113..379)
                     /locus_tag="OO327_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR6019235.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            653..4177
                     /gene="nifJ"
                     /locus_tag="OO327_002756"
     CDS             653..4177
                     /gene="nifJ"
                     /locus_tag="OO327_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR6019236.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4544..5673
                     /gene="ompN"
                     /locus_tag="OO327_002757"
                     /pseudo
     CDS             4544..5673
                     /gene="ompN"
                     /locus_tag="OO327_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5814..6248
                     /gene="uspF"
                     /locus_tag="OO327_002758"
     CDS             5814..6248
                     /gene="uspF"
                     /locus_tag="OO327_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR6019237.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6424..7359
                     /gene="ttcA"
                     /locus_tag="OO327_002759"
     CDS             6424..7359
                     /gene="ttcA"
                     /locus_tag="OO327_002759"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR6019238.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="OO327_002760"
     CDS             complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="OO327_002760"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR6019239.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="OO327_002761"
     CDS             complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="OO327_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR6019240.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            10571..11803
                     /gene="dgcM"
                     /locus_tag="OO327_002762"
                     /pseudo
     CDS             10571..11803
                     /gene="dgcM"
                     /locus_tag="OO327_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="OO327_002763"
                     /pseudo
     CDS             complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="OO327_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
     gene            complement(12641..14095)
                     /locus_tag="OO327_002764"
     CDS             complement(12641..14095)
                     /locus_tag="OO327_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCR6019241.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14092..>14479)
                     /locus_tag="OO327_002765"
     CDS             complement(14092..>14479)
                     /locus_tag="OO327_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6019242.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3861 a         3651 c         3417 g         3550 t
ORIGIN      
        1 acaccattag cgaaatgctg aaagaaggtg cacaagtgga tctgaccgcg aaccagttaa
       61 cgctggcgac cgcgaaacag acattaactt ataagctggc ggatttaatg aattaatagc
      121 tgccacagct cccggcggca agtgactgtt cgctacagcg tttgccgttg ggtaatgcac
      181 acatcccaat cgccgtacca tccagttgac gggcaacaga aagcgaaccg ccgatcattg
      241 cacaatttgc ttctccacta ctggacatcg acgcttttaa acttggcgct acgtgcgccg
      301 cagtcgcctg ctgaacaggc tcactactac acgccgacaa caataaagcg gcacacccta
      361 cccaaaacgc tgctcgcatc tcttcttcct ctgatcttca agccaaacga caccgccata
      421 aataataggc agcacagagg gcggcgtcga gagctgtcct gcgcgttgcc ccgcattttt
      481 acttttttat ggctattttt ttgccctctg tttgatcaaa acattcatta ctctgatgtg
      541 ggggacacaa aagcgaaaat gcagaagaaa gccatttgct aaaattgaag gattactact
      601 gggcgcgcag caatttcgtg cgcccctcat ttgcgcaatg taagggtgtc atatgattac
      661 tattgacggt aatggcgcgg ttgcttcggt gacgtttcgc atcagtgaag ttatcgccat
      721 ctaccctatt acccccagtt ccacgatggc agaacaggct gatgcctggg ccggaaacgg
      781 cttgaagaac gtttggggag acacaccacg cgtggttgaa atgcagtcgg aagcgggtgc
      841 tatcgctacc gtgcatggcg ctttgcagac gggtgccctt tcaacatcgt ttacgtcatc
      901 gcagggtttg ctgctgatga tcccgacgct gtacaaactg gcaggcgaac tgacgccatt
      961 tgtcctgcat gtagcggcac gtaccgttgc cacacatgca ctttctattt ttggcgatca
     1021 ttccgacgtt atggcggtgc gccagacggg ttgcgcgatg ttgtgtgcgg ctaacgtcca
     1081 ggaagcgcaa gactttgctc tcatttcgca catcgcaacg ctgaagagcc gcgtgccatt
     1141 tattcatttc tttgatggtt ttcgcacgtc ccacgaaatc aataaaattg tcccgctggc
     1201 cgatgacact attcttgatc tcatgccgca ggccgaaatt gatgctcatc gcgcccgggc
     1261 gctcaacccg gaacatccgg tgatccgcgg tacgtccgcc aatcctgaca cttatttcca
     1321 gtctcgcgaa gccaccaatc cctggtacaa cgcggtctat gaccatgttg aacaggcgat
     1381 gaatgatttc gctgccgcga caggtcgtca gtatcagccg tttgagtatt acgggcatcc
     1441 gcaagcggaa cgggtgatta tcctgatggg ctctgccatt ggcacctgtg aagaagtggt
     1501 tgatgaattg ctaacccgtg gcgaaaaagt tggcgtgctg aaagttcgtc tgtaccgtcc
     1561 cttctccgcc aaacacttgc tgcaagctct gccgggatcc gtacgcagcg tggcggtact
     1621 ggacagaacc aaagaacccg gtgcccaggc agaaccgctc tatctggatg tgatgaccgc
     1681 actggcagaa gcctttaata atggtgagcg cgaaaccctg ccccgtgtca ttggtgggcg
     1741 ctatggtctt tcatccaaag aatttggccc ggactgtgta ctggcggtat ttgccgagct
     1801 caacgcggct aaacctaaag cacgctttac ggttggtatt tacgatgatg tgaccaatct
     1861 gtcactgccg ttgccggaaa acactctgcc aaactcggcg aaacttgaag ccttgtttta
     1921 tggccttggt agtgatggca gcgtttccgc aaccaaaaac aatatcaaga ttatcggtaa
     1981 ttccacgccg tggtacgcac agggctattt tgtttacgac tccaaaaaag cgggcggcct
     2041 gacggtttct caccttcgcg tgagcgaaca gccgattcgt tccgcttatc tcatttccca
     2101 ggctgatttt gttggctgcc accagttgca gtttatcgat aaatatcaga tggctgagcg
     2161 tttaaaacct agcggcattt tcctgcttaa tacgccgtac agcgcagatg aagtgtggtc
     2221 gcgcttgccg caggaagttc aggccgtgtt aaaccagaaa aaagcgcgct tctatgtgat
     2281 taacgcggcg aaaatcgccc gcgaatgtgg cctggcggcc cgtattaata ccgtcatgca
     2341 gatggctttt ttccatctga cgcaaattct gcctggcgat agcgccctcg cagaattgca
     2401 gggtgcgatt gccaaaagtt acagtagcaa aggtcaggat ctggtggagc gcaactggca
     2461 ggctctggcg ctggcgcgtg aatccgtaga agaagttccg ttgcaaccgg taaatccgca
     2521 cagcgccaat cgaccgccag tggtttccga tgccgcccct gatttcgtga aaaccgtaac
     2581 cgcggcgatg ctcgccgggc ttggtgatgc cctccccgtt tcggcgctgc cgccagacgg
     2641 cacctggccg atgggcacca ctcgctggga aaaacgcaat atcgccgaag agatccccat
     2701 ctggaaagag gaactctgta cccaatgtaa ccactgcgtc gccgcttgcc cacactcagc
     2761 tattcgcgca aaagtggtac cgcctgaagc gatggaaaac gcccctgcca gcctgcattc
     2821 gctagatgtg aaatcgcgtg atatgcgcgg gcagaaatat gtcttgcagg tggcaccgga
     2881 agattgcacc ggttgtaacc tgtgcgtcga agtttgcccg gcgaaagacc gtcagaatcc
     2941 agagattaaa gccatcaata tgatgtctcg ccaggaacat gtcgaagaag agaaaatcaa
     3001 ttacgatttc ttcctcaacc tgccagaaat cgaccgtagc aaactggaac gtattgatat
     3061 tcgtacatcg cagctgatta caccgctgtt tgaatattca ggtgcttgcc ccggttgtgg
     3121 cgagacgccg tatattaaat tgctgactca gctctatggt gaccggatgt tgatcgctaa
     3181 cgccaccggc tgttcttcaa tttatggcgg taacctgccc tctacaccgt ataccaccga
     3241 tgccaacggt cgtgggccgg catgggcgaa ctctctattt gaagataatg ccgaatttgg
     3301 ccttggtttc cgcctgacgg tcgatcaaca ccgtgtccgc gtgctgcgtc tgctggatca
     3361 atttgccgat aaaatcccgg cggaattact gacggcgttg aaatcagacg ctacgccaga
     3421 ggttcgtcga gaacaggtcg cagctttacg ccagcaactc aacgatgttg ccgaagcaca
     3481 tgaacttctg cgtgacgcag atgcactggt ggaaaaatca atctggctga ttggtggcga
     3541 cggctgggct tacgatatcg gctttggcgg tctggatcat gtattgagtt tgacggaaaa
     3601 cgtcaacatt ctggtgctgg atacgcaatg ctattccaac accggtggtc aggcgtcgaa
     3661 agcgacaccg ctgggtgcag taactaagtt cggcgagcac ggcaaacgta aagcgcgtaa
     3721 agatcttggc gtcagtatga tgatgtacgg tcatgtttat gtggcgcaga tttctctcgg
     3781 cgcgcagctg aaccagacgg tgaaagcgat tcaggaagcg gaagcgtatc cgggaccatc
     3841 gctgatcatt gcttatagcc cgtgtgaaga gcatggttac gatctggctc tcagccacga
     3901 ccagatgcgc caactcactg ctaccggctt ctggccgcta tatcgctttg atccgcgtcg
     3961 tgccgatgaa ggcaaactgc cgctggcgct ggattcacgc ccgccgtcag aagcactgga
     4021 agaaacgtta cttcacgagc agcgtttccg tcgtctgaat tcgcagcagc cagaagtggc
     4081 agaacagtta tggaaagatg ctgcagctaa tttgcaaaaa cgctatgact tcctagcaca
     4141 aatggccgga aaagcggaaa aaagcaacac cgattaaatg ctctggataa ggattatcca
     4201 attctaaaaa aaaagcccgg acgactgttc gggcttgtct ttttatatgt tggaaaatca
     4261 gtggcaatgc aatggcccaa cagaaaataa aatctgtgac aataaaggca tataacccgc
     4321 gcagaataac gtataggaaa ttaaaagtat aatttttatt ttgtatgtat tatcttcgat
     4381 acattcacca gacttattat catttcagag gaattatttg attaaggttt tacttaaggc
     4441 gtaacaaatg atatttatcg gctaactgaa cttctccttt aggatgtttt cactccccga
     4501 aatggggata aaaggcaaat aaaataacta aaggatttat tcaatgaaaa gaaaagtact
     4561 ggcactttta attcctgccc tgctcgccgc aggtgctgca catgcagccg aagtttataa
     4621 taaagacggc aacaaattag atctgtatgg caaagttgat ggcctgcatt atttttctga
     4681 taattcagcg aaagatggcg accagagcta tgcgcgtctg ggttttaaag gcgaaaccca
     4741 aattaacgat caacttactg gctacggtca atgggaatac aatattcagg caaacaacac
     4801 tgaatcttca aaaaaccagt catggacccg tctggcattt gccggactga aatttgcaga
     4861 ttacggttct ttcgattacg gacgtaatta tggcgtaatg tacgacatcg aaggctggac
     4921 cgatatgctg cctgaatttg gcggtgactc ttataccaat gcagacaact ttatgactgg
     4981 tcgagccaat ggcgtcgcga cttatcgtaa tactgatttc ttcggtctgg taaatggtct
     5041 gaacttcgcg gtgcagtatc aaggtaacaa cgaaggagcc agtaatggtc aggaaggcac
     5101 caataacgaa cgcgatgttc gtcatgaaaa cggtgacggc tggggtcttt ccacaacata
     5161 tgatttaggc atgggcttta gcgctggtgc ggcatacacc tcttctgacc gcaccaatga
     5221 ccaggttaac catactgcgg cgggtggtga taaagcagac gcgtggactg ctgggctaaa
     5281 atacgatgct aacaatattt acctagcaac catgtattca gaaacgcgta atatgacccc
     5341 gtttggcgac agcgattatg cagttgcgaa caaaacccag aactttgaag ttactagtac
     5401 cagtttgatt tttgtctgcg tccggcagtc tctttcctga tgtctaaagg ccgtgacctt
     5461 cacgctgctg gtggtgcaga caacccggca ggtgttgatg ataaagatct ggttaaatac
     5521 gccgatgttg gcgcgactta ctatttcaat aaaaacatgt ccacctatgt tgactataaa
     5581 atcaacctgt tggatgaaga tgacagtttc tacgctgcca acggcatctc taccgatgat
     5641 attgtcgctt taggtctggt ttatcagttc taaatcctcc tgcccgctgt tatggcgggc
     5701 tttttctgct tattcttcct cctttgatct aaattaaaaa tgtgaactcc gtcattacac
     5761 aaaaagtgtc atctggcgtt acactttatg cggatactaa aacaggaggt tttatgaaca
     5821 gaacgattct tgtccctatc gatatttcag attcagaatt aactcaacgc gtgattagcc
     5881 acgttgaggc agaggcaaag attgatgatg cagaggttca tttcctgacg gtaatacctt
     5941 cactgcccta ctatgcctct ctgggtttag cgtattccgc agaattaccg gcaatggatg
     6001 acctgaaagc ggaagccaaa tcgcaactgg aagagatcat taaaaaattt aaactgccaa
     6061 ccgacagagt gcatgtccat gttgaggaag gctcgcccaa agaccgcatt ctggaattgg
     6121 cgaagaagat ccccgctcat atgatcatca ttgcttccca tcgaccggat atcaccactt
     6181 atctgctcgg ttccaacgcc gcagctgtag tgcgtcacgc agagtgctcc gtgctggttg
     6241 tgcgctgaca ctaacgcccg cacattgctg cgggcttttt gattcatttc gcaaatgtgc
     6301 tgacattttc ccctctaatc cgtaccatac acgccacagt ttttatatca gatttcttat
     6361 gctgagcgtt ccggcatgac gcatactctt ctgatgccat ataacgaatt gagtcgcttt
     6421 taaatgtcgc aaaatcaaga aattagtaag aaagaacaat acaacctgaa caaattacaa
     6481 aaacgtctgc gtcgtaacgt gggcgaagcc attgctgact tcaatatgat tgaagaaggc
     6541 gatcgcatca tggtttgcct ctccgggggt aaagacagct ataccatgct ggagattctg
     6601 cgcaatttgc agcaaagcgc gccaatcaat ttttcgctgg tggctgttaa cctcgatcaa
     6661 aagcaaccgg gcttcccgga acacgttctg cccgagtatc ttgaaaagct gggcgttgag
     6721 tacaagattg ttgaagagaa cacttacggt atcgtgaaag agaagattcc agagggcaaa
     6781 accacttgct cactgtgttc tcggctacgt cgcggtatcc tttatcgcac cgcaacggaa
     6841 ctggggacga cgaagatcgc gttgggtcac catcgtgacg atatcctgca aacgttgttc
     6901 ttaaatatgt tctacggcgg caagatgaaa ggtatgcctc cgaaactgat gagcgatgat
     6961 ggcaaacata tcgttattcg tccactggcc tactgccgcg agaaagatat tcagcgattt
     7021 gccgatgcaa aagcgttccc gattattccg tgcaacctgt gcggttcaca gcctaacctg
     7081 caacgtcagg tgattgctga catgttgcgt gactgggata aacgttatcc agggcgtatc
     7141 gagacgatgt tcagcgcgat gcagaatgtg gtgccgtcgc atctgtgcga taccaacctg
     7201 ttcgatttca aaggcattac ccacggttct gaagtggtta acgggggtga tctggcgttt
     7261 gatcgcgaag agatcccact acaaccggcg ggctggcagc cagaagaaga tgaaaatcag
     7321 ttggatgagt tacggctgaa tgtggttgaa gtgaaataac caggatagcg cccgatgcgc
     7381 aagcgtatcg ggctactctt atggaggccg gataagacgc ggccagcgtc gcatctggca
     7441 atcccgaata agatgtttac tcttgcaccc ggcaattcaa catttcatta ttttaataac
     7501 cgcacccggc acgtttttcc tttaatcttc ccgccctgta actgtttcca tgctttatga
     7561 gcaacagcct gacggaccgc gacatagaca tgcgccggat gcacggcgat tttgccaata
     7621 tctgcgccat caagcccgat atctcctgtc agtgcaccta atacatcacc cgggcgcatt
     7681 ttggcttttt tcccgccatc gatacacaac gttgccattt ctgcttccag cgtcacaatg
     7741 gaactattag ctggcggcgt ttgccagtta agttttatct gcaacatgtc agaaatgata
     7801 ttggcccgct gtgcttcttc cggagcacag aaactgatcg ccagaccgct atttcctgca
     7861 cgagctgtac gaccgatgcg atgtacatga acttcagggt cccacgccag ctcaaagttc
     7921 accaccagct caagcgattt aatatccaga ccacgcgcag caacatcagt cgcaaccagt
     7981 acacgggcgc taccgttagc aaaacgtacc agggtctgat cgcgttgctc cagatcgccg
     8041 tgtaacgaca atgcactttg ctctacttca ttcagcgcgt cgcagacagc ctggcaatct
     8101 tttttggtat tgcaaaacac cacacaagag gatggctgat gcaagcttaa taaccgttgc
     8161 aacagaggaa ttttgccttt actggatgtc tcataaaatt gttgttcaat gggtggcaaa
     8221 gcatctgttg agtcaatttc aatcgccaaa ggatcgcgtt gcactcgtcc gctgattgcg
     8281 gcgatggctt ccggccaggt tgccgaaaac agaagcgtct gtcgagatgc aggcgcaaaa
     8341 cggatgacat catcaatggc atcgctaaat cccatatcca gcatgcggtc ggcctcatcc
     8401 atcaccaaag tattcagcgc atccagtgat accgtgcctt tttgcaagtg atccagcaaa
     8461 cgacccggcg ttgccacgat aatatgcggc gcatgttgca gcgaatcacg ctgcataccg
     8521 aacggttgac caccgcacaa cgtcaaaatt ttggtatttg gcagaaaacg cgccagccga
     8581 cgcaattcac ctgccacctg atccgccagt tcacgcgtag gacacagcac taaagcctgg
     8641 gtttgaaata gcgacgcatc aatttgctgt aacaagccga ggccaaaagc cgccgttttg
     8701 ccgctgccgg ttttcgcctg cacgcgaaca tcttttccgg caaggatcgc cggaagcgcg
     8761 gcggcctgca ccggcgtcat ggttaaataa cccaactcat taaggttcgt gagttgggcg
     8821 ggaggcaaaa cattcagggt agaaaaagcg gtcacaatct attctcgtgg tcatcgacgc
     8881 aaagttagca ggcgcgtatc ctcgcagatc tacgctcacg atgcgacaat ttaatcggtt
     8941 cttcatcggg tggtgggtca ggcatgggtt gcgggcgagg gatcggatcg ggcactggaa
     9001 caggatcgcc aggaatcggt tcagggacag gaatttgcaa ataaataagt gtcgtcatat
     9061 ttccctccgg tcattgggtg gactcttaaa gggtagacgc tgataaataa caggcaaaaa
     9121 aaagccgact catcaaagtc ggcgtcgtac gaatcaattg tgctatgcag taattcaaaa
     9181 aaggaagtaa gacaatatgg agcgcaacgc ccatcgcttg acgttgcatt cacctgcaag
     9241 ggagatattg ccctgaatgg gtagagagtt tattgacttc gctcaaactt tgcggcgttt
     9301 ttgtatacag acagccggaa aaattgcttt tgttacaacc atttactacg gtgcaaccat
     9361 aaagcaacac caccaataag aacaactaac agaatacaaa aaattgaaaa tccgaattgc
     9421 cacccgccgc cagggatccc accaaggttg acgccaaata acccggtcag aaaggtactg
     9481 ggtaaaaaga ccattgccat caacgacatt gtataggtac gacgagctaa attttcctgc
     9541 atcacctgag cgatttcatc cgccatcacg ccagtccgtg ctatacaggc gtcgatttcg
     9601 tcaaggccgc gcccaaggcg atcggcaata tcctgcatcc gacggcgttg gtcatcgctc
     9661 atccacggca aacgttcact ggcaagacga gcataaacat cacgttgcgg tgccatatag
     9721 cgacgcatca caattaattg tttgcgcagc agagccagga atccacgcgg tggaatttgc
     9781 tgatcaagga gattatcttc aaggtcgata attttatcgt gcagctgctc gataaattca
     9841 ctggaatgat cggtcaacgc atcgcacaca tccaccagcc atcccccgca atcggtcgga
     9901 cccgtgccct cttccagatc gctcaccaca tcgtccagcg ccagcacttt gcgttgtcgg
     9961 gtcgaaacaa ttaaccgccc gtccatatat acacgcatgg cgaccagttg atcggggcgt
    10021 tcatcggtgc tgccgtttat acagcgcaat gtaatcagcg tgccttcacc gaaacggctg
    10081 actcggggcc gtgtgctctc gcccgccagc gcatcacgta cgttattggg aagcagcggt
    10141 gttgtcgcca gccattgggc gctatcatgg tgtacataat taaggtggag ccagcaggga
    10201 tgcgcttcat caatcacatc tgtattttcc agcggtttaa cgccgcctct accatccagc
    10261 atccaggcaa atactgcatc cgggacatta acgtccgatc ccttaatcgc ttccactgtg
    10321 cctccatcat caacgcatta ttttgtagtc tagccttctg gccctgttac gcaacatctc
    10381 atcaccccat taccctgaaa tgattaataa aattctgtct aaattgaata caaaaagcaa
    10441 aatgcttttc cgtatacaaa ccgtgtgaag tgttaaatag cgtctatcat tatcagaatt
    10501 atctgatcat atgacgtggc ttttttgcga tcggatagca acaaaaattg ataaaaataa
    10561 cgggatctca atgattacgc acaacttcaa taccctggac ttactcacca gtcctgtctg
    10621 gattgtttcg ccctttgagg aacagttaat ttatgccaat agcgcggcgc gactgttgat
    10681 gcaagacctc acgtttagtc agctacgaac cggaccctat tccgtctcct cacaaaaaga
    10741 actgccgaaa tacctctccg atctgcaaaa ccaacacgat attatcgaaa tcctcactgt
    10801 tcagcgtaaa gaagaggaaa cagcattaag ctgtcggctt gttttgcgag agctgacaga
    10861 aacagaaccg gtgattattt tcgaaggtat cgaagcgccg gcaacgctgg gtttaaaagc
    10921 cagtcgctcg gcaaattatc agcgcaaaaa acaaggtttt tatgcgcgct tttttctgac
    10981 taactctgca ccaatgttgt tgattgaccc gtcacgagat ggacaaatcg tcgatgctaa
    11041 cctcgccgcg ctcaatttct atggttataa ccatgaaacg atgtgccaga aacataccta
    11101 ggaaataaat atgcttgggc gtcacgtcat gcctatcatg catgaaattt cgcatttacc
    11161 cggcggtcat aaccctttga attttgttca taaactggcg gatggatcta ctcgtcatgt
    11221 gcagacctat gccggaccga ttgaaattta tggcgacaag ctcatgttat gtattgtgca
    11281 tgatattact gagcaaaaac ggctggagga gcagctggaa catgctgctc accatgacgc
    11341 gatgaccgga ttactgaatc ggcgacagtt ttatcacatt acggaaccag gccaaatgca
    11401 gcatctcgcc atcgctcagg attacagctt gttgctcatc gacaccgatc gttttaaaca
    11461 cattaacgat ctctatgggc attctaaagg tgatgaggtg ttatgcgccc tcgcccgccc
    11521 cctcgaaagt tgcgctcgca aaggcgattt ggtgtttcgt tggggaggcg aagagtttgt
    11581 cttattgcta ccaagaaccc cactggatac cgcgctttcg ctggctgaaa ctatccgcgt
    11641 aagcgtggca aaagtgagta tttcgggctt accacgcttt accgtcagca ttggtgtggc
    11701 gcatcacgaa ggaaatgaaa gcatcgatga actgtttaaa cgcgttgatg atgctttgta
    11761 tcgggcgaaa aatgatggac gcaaccgcgt gctggcggca taagccgcgg atgcgtctcg
    11821 agatcaacga ctgcgcttag cgtggcgctc ccagttttct tgcttcgcct gcgccgtttt
    11881 acgtagtgcg acgtaacacg ccccgctgcc gccatgatgc ggtagcgcgg tgcaatatgc
    11941 ctgaacatca tcaaattcgg tcagccagcg cgccacatag ctgcggacaa tattggcatg
    12001 tgatttatct tcccgccctt taccatgaat aatcagcaca ttacgcaaac catccgccag
    12061 ggcttgttga atgaaactga acaccatttt gcggcactct tccaccggct ggcgcaaaag
    12121 attcaggctc gcctgttgcg gatatttacc actgcgcagc ttatccagca ccccatgcta
    12181 caacccttcc tgccgaaact ccagcggctg acttagcggg atgatgtcga gaaatccggt
    12241 agtgaggaaa ttatcaagct gcagcgtgtc gatacgctgc ggcgcacgtt ggttacgcgt
    12301 tggatgccag tggacatctg tagcacgttt cagcggctgg acatcttcca tggcgtcaag
    12361 aaacagcgat ttgtcgtcaa ggttcatgta acctcctatg caccttcaca cgtcgtgatt
    12421 cagacgggtt aattgttcat acttactgca tcgttccctt cacagattga aacaatactc
    12481 tacttcatac ctgtgagtat ctgcgcaggc ggggcatttt atactagcct gttagcagta
    12541 tcgaacagtt tagcccgagt ttaattcagc acaggctccg atcaggatga tttattcata
    12601 gcaggaacca acaaaaatgc gataaataac agaccactga ttagcagaaa ggtaccgaaa
    12661 atgccatagt ttgcgcctcc ctcagcatca attgactcaa taatttggtt gaagccagta
    12721 tttccgatgt ctggtcactt ctactgcgat taaaaaataa agcacttctg gaaatgaagt
    12781 cattcaccgt atggggaaca gaaaccattt tttcacccat tctcgaaaaa tgaaaattag
    12841 ttcgattatt gagaatagag ccacacaggg caataccaat cgcgccccca agattacgtg
    12901 ttaaattaaa tacaccagag gctaacttca gccgttcttt tggaattccc cccagcgtta
    12961 atgttacgat gggggccatc gcaaactgct gcgaaatccc tctaattgcc tgtggaaaca
    13021 gtaattcctg ccatccccat tcgtgggtta taggggtaaa aagatacatt gaaaacacaa
    13081 atccgcccag ccccgccatc aacagccatc gcagatttat ttttttactt aaccagaagt
    13141 aaaagggaac agaaaataac tggaaaatac ctgttgtgca aacggcaaaa cctatctctt
    13201 cggcatttaa tccgcgaatc tgccccaaaa acacaggtat taaatataca gtagagaata
    13261 tcccaacccc tccagaaaaa gaaaaaaaac atcccagagt gaaattttta tccttaaaag
    13321 catgaagatc cattatcgga ttggatattg taagtgtacg cgcagcaaat aaaataaaac
    13381 tcaccaatgc cagaactgat gtcagcaaaa tagtattatc atccagccat ccccagcgcg
    13441 ctccttcttc aagggtatat tccagacatc ccagagtcat ggccaacagg ataatactcg
    13501 gataatcagc aactttcagt aaagagagat caggtttatc aaaattaacc agaaaaggaa
    13561 tactcaaaac taaatatatg ccaggtaata tattgatata aaatagccat cgccagtcga
    13621 ggttatcagt gagccagccc cccagagttg gccctagcgt cggtgacagt gaggccagcg
    13681 cactgactac cgcagcagcc aaccccaact ctttaccctg gtaataaata aacgccatcg
    13741 tgaaaactaa agggatcatg gatgcgccag cagctccctg taaagcacga aacaaaatca
    13801 tgatctgaat attccatgca agtccacacg cgatactcat taaagtaaat attcccgcgg
    13861 atagagtaaa aagccaacga gtggaaaata cccgtgacag ccacccagag agcggaataa
    13921 caataatctc agcaattaaa tagctggtct gaagccaggc catttcgtct ttacctgcgg
    13981 acagaccacc acctatttca tttagggatg acgatacaat ttgaatatca agctgagcaa
    14041 taaataatcc catacacata ctggcgaaag caatatgttt acgcatagac atcatctctc
    14101 atcaacactg acggtgacgg acagtcccgg gcgaagaagt tgctttaatt cctttgcgtc
    14161 gtcaaatgca atccgtaccg ggacacgctg aacaattttg gtgaaattgc cagtggcatt
    14221 ttctatcgga atcaagctga aactggctcc tgtcgctggg gaaaggcttt caatgtgacc
    14281 gtgaaacact tcccctttga gaatatcagc acggatctca gccttcattc ctggcttcat
    14341 tcccgctatt tggttttctt tataatttgc atcaacccac aactcactga ctggtactag
    14401 tgaaactaac gatgtgccgc cttccaccca actgcctgta tgggcgctgc ggtttgcgac
    14461 aatgccatca atcggcgca
//
LOCUS       DAJTSI010000100        14316 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000100 DAJTSI010000000
VERSION     DAJTSI010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14316
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.100"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..3051
                     /locus_tag="OO327_002766"
                     /pseudo
     CDS             1..3051
                     /locus_tag="OO327_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3061..6707
                     /locus_tag="OO327_002767"
                     /pseudo
     CDS             3061..6707
                     /locus_tag="OO327_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6768..7175
                     /locus_tag="OO327_002768"
     CDS             6768..7175
                     /locus_tag="OO327_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019243.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            7717..8805
                     /locus_tag="OO327_002769"
     CDS             7717..8805
                     /locus_tag="OO327_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCR6019244.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8816..11095
                     /locus_tag="OO327_002770"
     CDS             8816..11095
                     /locus_tag="OO327_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019245.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLLEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            11088..12224
                     /locus_tag="OO327_002771"
     CDS             11088..12224
                     /locus_tag="OO327_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR6019246.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            12221..14224
                     /locus_tag="OO327_002772"
     CDS             12221..14224
                     /locus_tag="OO327_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019247.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
BASE COUNT         3546 a         3755 c         3655 g         3360 t
ORIGIN      
        1 gggattatgc gaataatgtc attattattc tgccgtctgg cattgcacct gactacttgg
       61 agcaatattc cagatttgaa ttacgcctac gtaaacattt atcactggcg gaagagtctc
      121 tctggcaaaa atgtgcacaa aaacttattg ccgcaattcc acatattcca gaatggcggc
      181 aaccattaat tgctttgtta ttacccgaaa aaccagaaat tgcacatgaa attgcccagc
      241 atctactggg gcaaaaaaaa ttaccctcgc ttgagtggtt aaaaatagtg gcgactgatg
      301 agcacattct tgcctcatta gaaaaatatc acgagccata tgccattttt gatgattact
      361 attgtggtgc gatatggtca gccaccgtat tacaggagca aggtgttgca gccctgcccc
      421 gatttgctcc ctatgccgca agtgactact gcgccgatgt gttgcgtcat atcaatcatc
      481 cgttcgcatt aacactgctt atacgtgtag ccgggcatac taaacgctgt cacgatcgaa
      541 tgacgaaagc ctgcgctgcg ttcccacacg cagcactggc agcactggcg gaacttctag
      601 tgcaaaaaga agagaatagt tggcgcatta tgctaatgac gatgtttatc tcgcaaccag
      661 cactggcaga acaggtcatt ccctggcttt cgacacccgc agttgcagtg ctgaaatcat
      721 gccagcacca actgacacag ccctcaaacc atgccagcgc cgatctactg ccagccatag
      781 tagtctcccc accatggatt tcgaaaaaga aaaaatcgcc gattccggtg ctggatttag
      841 cgccattagg catcgagcca atctgttatc tgacagaaga aatcagtaat caacttttgg
      901 cgaaatatat ctggtattca aaacacatca cggttagcca tgaagaaagt actaccaacc
      961 tgttggcaag gatgggtttt caacgacgga tcgctggtac atatattaaa gctcccgaag
     1021 cggtagttga ggcatggcta aatgaagatt attcaacctt actaagtgaa tttaaggtgt
     1081 ttcattcacc taccgggcat tattggcagt tggggatttt gacaacattg ccgctggagg
     1141 ctggagaaag cagtaaaagc atggaatgcc cttactctat ctccacatac cgataccgaa
     1201 tacgccatgt tacattttgg actcaaaggt ttacctggtt tagttaactc aattacacgc
     1261 tatccacagg aagccttgcc catcacgaat tacttcgcag cgagtgagct ggctcctgcc
     1321 gtcgcccgtg ccttcaacaa actcaaaacg ctacgcgaaa acgcccgcac ctggctgttg
     1381 aaatacccgg aacatgccct taccggcctg ctgcctgcgg cgctcggcaa agccggtgaa
     1441 gcacaggata acgcccgcgc tgccttgcgt atgcttaccg aaaacggtca tcagccatta
     1501 ctgcaagaaa tcgcccggcg ttataaccag tcggaagtaa cagacgcggt gaacgctctg
     1561 cttgcgctcg atcccttaga taatcacccg acaaaaatcc ccactcttcc gaccttttat
     1621 cagccatcga tctggacgcg cccggtatta aaagcaaacg cccaatcact gccagataac
     1681 gccctcctcc acctcggcga aatgctccgc ttccctcagg aagaggctct gtatccggga
     1741 ttattgcagg tgaaagacgc ctgtaccgct gactctctgg ctgaatttgc ctgggatctg
     1801 tttaccgcct ggcagaccgc tggcgcgccg tcgaaagaga gttgggcgtt cactgcgtta
     1861 ggcgttctcg gtaacgatga caccgcccgc aaactgacgc cattaatacg cgcctggcct
     1921 ggtgaatccc agcataaacg cgccaccgtt gggttggata ttctcgccgc tatcggtagt
     1981 gatatcgccc tgatgcagct taacggcatt gctcagaaac tgaaattcaa agcattacag
     2041 gagcgggcaa aagaaaagat tgccgacatt gccgagagcc gcgaactcac ggtagcggag
     2101 cttgaagatc ggttagcacc ggatctcggt ctggatgata acggttcgtt gctgctggat
     2161 tttggcccac gccagttcac cgtcagcttt gatgaaacct taaaaccgtt tgtgcgtgat
     2221 gtttccggca gccgcctgaa agacctgcca aaaccaaaca aaagcgatta tgagtcacag
     2281 gcgaacgatg ctgttaaccg ctacaaattg ctgaaaaaag atgcgcgtac cgtcgcggcc
     2341 cagcaggtag caaggcttga atccgccatg tgcctgcgtc gccgctggtc gccggaaaac
     2401 ttccaactct tcctggttgt gcatccgctg gttcgccact taacccgccg tctgatttgg
     2461 ggcgtttata gcgccgacaa ccagctactg gcctgtttcc gggtagcgga agataacagc
     2521 tacagcaccg ctgacgatga tcttttcacc ctgccggaag gcgatatctc tatcggcatt
     2581 cctcacgttc tggaaatatc accgacggat gctgtcgcct ttggtcagct ttttgccgac
     2641 tacgaactgc taccaccgtt ccgtcagctt gaccgtaaca gttacgccct gacagaagcc
     2701 gagcgcaacg ccagcgaact gacccgctgg gcaggcagaa aatgcccgag cggccgggtc
     2761 atggggctgg caaataaagg ctggataaag ggcgaaccac aggatggagg ctggatgatc
     2821 aaacctctcg gtcgctggtc gttaatcatg gaaatcgacg aaggttttgc ggtgggtata
     2881 tcaccagccg aacttagcgc tgagcagctc ttaagcaagc tgtggctatg ggaaggcaaa
     2941 gcggaaagct atggctgggg gagtaattca acacaggaag cgcagttctc cgtactcgat
     3001 gccatcaccg ccagcgagct aattaacgat attgaagccc tgtttgaata aggaaagagc
     3061 atggacaagg aattaccgtg gctggcggat aacgcccaac tggaactgaa atataaaaaa
     3121 ggcaaaacac cactcagtca tcgcaattgg ccgggcgaac cagtgcctgt tatcactgaa
     3181 agcatcattc agacactggg tgatgaattg ctacaaaaag ctgagaagaa aaaaaacatt
     3241 gtctggcgtt atgaaaattt ttcaccggag tggcagtccg ccattacgca ggccatcaac
     3301 ttgatcggcg aacacaaacc ctcagtcccg gcccgcacaa tggcggcact agcctttatc
     3361 gcgcaaaatg acaaccaaca gttgctcgac gaaatcgtcc aacaagaggg gctggaatat
     3421 gcgactgagg tggtgattgc acgccagttt attgcacggt gttatgagag tgatcctctg
     3481 gtagtcacat tgcagtatca gaacgaggac tatggctatg gttatcgctc agaaacctat
     3541 aacgaattcg atctccgact gcgtaaacac ctctctctgg cagaggaaag ctgctggcag
     3601 cgttgcgccg acaaactcat tgccgcactg ccaggaataa ccaaagttcg ccgccctttt
     3661 attgcgctga tcctccccga aaaaccagaa atcgccaatg agttggtaag ccttgaatgc
     3721 ccgcggactc attttcattc taaggagtgg ttaaaagttg ttgctactga ccccaaagcg
     3781 gtgagaaaac tcgaacgcta ctggagccag gatatattta gcgatcgaga agccagctac
     3841 atgtcgcatg aaaaccactt cggctacgcg gcctgcgctg cccttttgcg cgaacaagga
     3901 ctggcagtcg ttccgcgcct cgcgatatat gcccataaag aagattgcgg cagtctgctg
     3961 gtacaaatta accatccgca agtcatccgc accttgctgt tagttgctga taaaaacaaa
     4021 cccagcctgc aacgtgtggc taaatacagt aaaaacttcc cccatgcgac actcgccgca
     4081 ctggcagaac tgctggcgtt aaaagaacca ccagcccgcc caggttatcc aatcatcgaa
     4141 gacaaaaagc tgcctgcaca gcaaaaagct cgagatgaat actggcgtac gctgttacaa
     4201 acgctgatgg catcgcagcc acaactggca gcagaagtga tgccgtggtt aagtactcaa
     4261 gcccgggcag tgctgaatag ttatttatcg gcaccgccca aaccagttat tgatagcacc
     4321 gataacagcc aaatgcctga aatcctcgtt tcaccaccat ggcgtgggag aaagaaaaag
     4381 acttccatac gtcacttttc gctgcctgat cttacgcttg caccgcaaac gtacaggtcg
     4441 ccgaataaac actggacgcc ggaacagcaa actgcaattg accattttag cgccatgtct
     4501 tttgacgaac gtcttgctac gagaggagcg gatatcttct tgcgggaatt aggttttgac
     4561 gttcatagcc tggaatttaa taagcgtctt cttaatggac agttggatgc ctttgatgct
     4621 cagcaactgc aagaatacac atcaatagga ctattgcgta cttatcacgc gcatcaggcc
     4681 aaatctaaac agtaccggga agacgccacc aatgcattac tggcacaaga tagcattgcg
     4741 ttgatgggag ggtggcgaac ttttcagcac ggattttact atggtaatga acagcgttgg
     4801 gggatttgga atctgtacct tatcgcgcag atgccgcgcg aaatggcggt atcgtgctgg
     4861 caacaaattg tcgaggctga ttttcattac accggggtgg aatatttgtt aagtgtgctg
     4921 ggtaccgatg ctttgcctgg attaaacacc gcctttgccc gacatccaaa agagatattt
     4981 ccgctgctga ttcatttcgg cgcaacagaa ctggccctgc ccattgcccg cgtctggcac
     5041 ctttttgcgg gccagcgtaa tctggctcgc cagtggattt tacaatggcc ggaacatacg
     5101 gctactgcac ttatccctct tgtcttcacc aaatctagcg ataaaagcga agctgcatta
     5161 cttgccctgc gtttacttta cgaacatggg catggcgaat ggctacaaac tgtagccaat
     5221 cgttggcagc gtacagatct atggcctgcc ctggaacaat tacttaaaca gagtccaata
     5281 gagatttacc cggcacgcat tccaaaagcc cctgatttct ggcatccgca aatgtggtcc
     5341 aggccgcgcc ttatcactaa taatcaacct gttaccgatg acgctctgga aattatcggc
     5401 gaaatgctgc gctttaccca ggggggacgt ttttatagcg ggctggaaca actgaaaacg
     5461 ttctgccagc cacaaacgct ggcagctttt gcctgggatc tcttcactgc gtggcaacaa
     5521 gctggtgccc ccgcaaaaga caactgggca tttctggcgt taagtctctt tggtgacgaa
     5581 agcacggcac gggatctaac gacacatatc ctcgcctggc cacaagaagg caaatctgcc
     5641 cgtgcggtca gtggcatgaa catccttacc ctgatgaata atgatatggc gctgatgcag
     5701 ctgcatcata tatcgcaacg ggcgaaatcc cgccccttac gtgataacgc ggcggaattt
     5761 cttcaggtag tcgcagaaaa tcgcgggcta agccaggaag agttagcgga cagattagtc
     5821 ccaacactgg gccttaatga tccgcaggcg ttgagttttg attttggtcc ccggcagttt
     5881 accgttcgct tcgatgaaaa cctcaacccg gttatctttg atcagcaaaa cgttcgccag
     5941 aaaagtgttc cccggttgcg cgccgatgac gatcaactga aagcgcccga ggcactggcc
     6001 cgactaaaag ggttaaaaaa agatgctact caggtgagca aaaacctgct cccgcgtctt
     6061 gaagctgccc tacgtaccac ccgacgctgg tcgctggcag attttcattc tctgtttgtt
     6121 aatcatccct ttacccgtct ggttacccag cgattaatat ggggggttat ccggcaaatg
     6181 aaccgcgtcg tttactcaac gcctttcgtg tggccgcaga gggggagttc tgcaatgcgc
     6241 aagatgagcc aattgacctg cctgcggacg ctctgattgg cattgcccac ccgttagaaa
     6301 tgacagcaga aatgcgcagt gaatttgcac agctttttgc cgattacgaa attatgccgc
     6361 cttttcgcca gttgtcgcgc cgcacggtgc tgctcacacc tgacgagtca accagtaaca
     6421 gcctgactcg ctgggaaggt aaatccgcta ccgttgggca acttatggga atgcgataca
     6481 aaggctggga gtcaggttat gaggacgcat ttgtctatga cctgggtgag taccggctgg
     6541 tccttaagtt ttcacccggt tttaaccact acaatgttga tagcaaagcg ctaatgagct
     6601 tccgttctct tcgagtgtac cgtgacaata aatccgtcac ttttgccgaa cttgatgtgt
     6661 ttgatttgag tgaggcgtta agcgcacctg acgtcatttt ccattaaaac aaagcgggca
     6721 gtgatgcgac tgcccgttat ctacacgact taccagcggg gaaagcgatg atcgtgcaaa
     6781 aagagctggt tgctatttac gattatgagg tccctgtacc tgaagatccg ttttccttca
     6841 gacttgagat ccataaatgc tctgaattat ttacaggttc cgtctaccga ctggaacggt
     6901 tccggctacg tccaacattt catcaacgtg atcgagagga tgttgacccg ctaataaatg
     6961 atgcgttgat ttatataaga gatgagttta ttgatgagca aaaattacga ggcgaatcac
     7021 ctgaacctgt aatagtaatt tttaattgta aactacagaa tatattcaac caacaaatag
     7081 aattatatac tctagataat tcaagtcgca gcacatgcaa cttgaagtat gacgagtata
     7141 gctttattaa aggggggaac ggtattcccc cttagatgcg tttcaatttg cccgttactt
     7201 caacaaatca atcaaccgta acagagccac taatagctcc agtacagcaa tgagcacggt
     7261 taacttttgt gtcagcgtgt ccatccgctc tccttattaa ggagcacaac accagcacct
     7321 tacccttaca ctgcctgtcg atactggttt acgtttgttg agtcgccatg ttgcactccg
     7381 gatcagagca ctacctgcgg caccacccgt ccggtagcca ggacttgaaa taggttcgat
     7441 ccggcgcaac ttcccaacag tcaggattat aaatctcatt atcttaattg caacactgtc
     7501 cagaggtggt tataattcgt tcgttggaac acgatgttgc acaggcagtg gtgttaggcc
     7561 tgaaaatagt aaataaaaga acctgcctca ccagcagttt tttttattta ctgtgatctg
     7621 ctttccagat atttttcgct caaacaacta atgcgccaaa catttattgc gcgtaaaata
     7681 tcgtttattt cattaataca tttcagggat gaatatatgt caccgcagaa taatcatctg
     7741 cagcgtccgc ctgctgctgt gttatacgcc gatgaactgg caaaattaaa acaaaatgat
     7801 aacgcacctt gcccgcccgg ttggcagtta agtttgcctg cggcccgtgc ttttatcctt
     7861 ggcgatagcg cgcaaaatat tagccgtaaa gtggtgatta gtccctccgc tgtcgaacgt
     7921 atgttagtca ctcttgccac cggacgcggt ttgatgttgg tgggggaacc tggcaccgca
     7981 aaatctctcc tgtctgaatt actggcaacc tctattagcg gcgatgccgg gttaaccatc
     8041 cagggcggcg catctactac cgaagatcaa atcaagtacg gctggaacta cgccctgctt
     8101 atcaatcatg gtccatcaac ggaagcgctg gtccctgccc cgctttacca gggaatgcgc
     8161 gacggcaaaa tcgtccgttt tgaagagata acgcgtacac cgctggaagt gcaagactgt
     8221 ctgttaggta tgctttctga cagggtgatg acggtgccgg aactcactgg tgaagccagt
     8281 cagctctatg cgcgagaagg ctttaatatt attgccactg ccaatacccg cgatcgcggc
     8341 gtcaatgaaa tgagtgctgc gctaaagcgt cgcttcgatt ttgaaactgt ctttccgatt
     8401 atggatttcg cccaggagct ggagcttgtc gccagtgctt cggcgcgttt gctggcccat
     8461 agcggtattc cgcataaagt gcctgacgcg gtactggagt tactggtccg caccttccgc
     8521 gatctgcgtg ccaacggcga aaagaaaacg tcaatggata cactaacagc gattatgtcc
     8581 accgctgaag ccgtgaacgt ggcccacgcc gtaggcgtcc gcgcctggtt tttagcgaat
     8641 cgcgcaggtg agccagccga cctggtggac tgcattgccg gaaccatcgt caaagataac
     8701 gaagaagatc gcgctcgtct gcgccgttac ttcgaacagc gtgtcgccac acataaagaa
     8761 gctcactggc aggcttatta tcaagcccgc caccgcctgc cgtgaggaaa gatgcatgag
     8821 cgagccgtta attgtcggca tccggcatca tagtccggcc tgcgcccggc tggtgaaatc
     8881 gttaatcgaa agccagcggc cacgatacgt gttgattgaa ggccctgctg attttaatga
     8941 ccgggtagac gaactgtttt tagcccacca gcttccggta gctatttaca gttattgcca
     9001 gtatcaggac ggtgcagccc ccgggcgtgg tgcctggacg ccatttgctg aattttcgcc
     9061 ggagtggcag gcgctacaag ccgatcgtcg tattcaggca caaacttact tcatcgattt
     9121 gccttgctgg gcgcagagtg aagaagagga cgattcgcct gatacgcaag aggaaagcca
     9181 gaccttgctg ctgcgtgcca cccgcatgga taacagcgac accctgtggg atcacttgtt
     9241 cgaagatgaa agccagcaaa ctgcattacc ctctgcactg gcgcactatt ttgcccaact
     9301 gcggggcgac tcccccggcg atgctctaaa tcgtcagcgc gaagccttta tggcccgctg
     9361 gattggatgg gcgatgcagc aaaataatgg cgacgtgtta gttgtctgcg gtggctggca
     9421 cgctccggca ctggcaaaaa tgtggcgcga atgtccgcag gtaattaaca cgccagaatt
     9481 gccgtcgctg gcagatgccg ttacaggttg ttatctcacc ccctacagtg aaaagcgcct
     9541 tgatgtgctg gcaggatacc tttcaggaat gcctgccccg gtctggcaaa actggtgctg
     9601 gcagtggggc ttacagcagg cgggagaaca actgctaaaa acggttctca cccgtttgcg
     9661 ccagcacaag ttgcctgctt cgaccgcgga tatggctgcc gctcatctgc atgcgatggc
     9721 actggcacag ttgcgcggtc atacactacc gttacgcact gactggctgg atgccatagc
     9781 aggatcgctg attaaagaag ccctgaatgc gccgttgccg tggagctatc gcggcgttat
     9841 tcatcccgat accgatccga ttctgctaac gttgatagac acattagcgg gtgacggatt
     9901 cggtaaactt gccccttcta cgccacaacc gcctctgcca aaagatgtca cttgcgaact
     9961 ggaacgtacc gcaatctctc ttccggcgga gcttacctta aatcgcttta accccaatgg
    10021 actagcgcaa agtcaggtgt tacatcggct ggcaatactg gagatcccag ggattgtacg
    10081 ccagcaggga agcacactga cacttgcagg caacggtgaa gaacgctgga aattaacccg
    10141 cccgcttagc cagcatgcgg cattgattga ggccgcctgc tttggtgcca cactcctgga
    10201 agccgcacgc cataaattag aagccgatat gctggacgcg ggtggaatcg gcagcattac
    10261 cacatgtctt agccaagcgg cgtcagcggg tctggcgtcc ttcagtcaac aattactgga
    10321 gcaactcaca ttattaatcg cccaggaaaa tcaatttgcc gaaatgggcc aggcgctgga
    10381 agtgctatat gccttatggc ggctggatga aattagcggt atgcaaggcg cgcagatatt
    10441 acaaacgacg ttatgcgcgg ctatcgatcg cacgctgtgg ctgtgtgaat ccaacggcag
    10501 accggatgaa aaggagtttc acgctcactt gcatagctgg caagcgttgt gtcatatcct
    10561 gcgcgatcta catagcggtg tgcaactccc cggcgtttcg ctttctgcgg cggtagcctt
    10621 gctggagcga cacagtcagg cgattcatgt cctggcactg gatcgcggcg cgactcttgg
    10681 tgcgctgatg cgtctggaac atcccaacgc tagtgccgaa gcggcgctga cgatgctggc
    10741 gcagttatcc ccggcacaat ccggcgaggc gctgcacggt ttgctggcat tagcccgtca
    10801 tcaactggcc tgtcagccgg catttatcgc cggtttcagc agtcatttaa atcaactaag
    10861 tgatgccgat tttaccaatg ccctgcccga tttacgcgcg gcaatggcct ggctaccgcc
    10921 acgagaacgc gggacgctgg cgcatcaggt gcttgagcat tatcaactgg cgcaacttcc
    10981 cgtttcggca ctgcaaatgc cgttgcattg tccaccgcaa gccattgcac atcatcaaca
    11041 actcgaacag caggcactgg catcgctgca acactgggga gttttccatg tctgaactga
    11101 acgatcttct gaccacccgt gagctacaac gctggcgatt aattcttggc gaagcggcag
    11161 aaacgacgct ttgtgggctg gatgacaacg cccggcagat agatcacgcg ctggagtggc
    11221 tgtatgggcg cgatcctgaa cggctccagc gtggtgaacg ctctagtgga ttaggtggct
    11281 caaatctcac cactcctgag tggatcaaca gtattcacac gctgtttccg caacaggtga
    11341 ttgagcggct ggaaagcgat gccgtactgc gctacggcat tgaagatgtg gtgacaaatc
    11401 tcgacgtgct ggaacgtatg cagccttccg aaagcctgct acgcgccgtt ttgcacacca
    11461 aacatctgat gaaccccgaa gtactggctg ccgcccgcag gattgtgcgc caggttgtgg
    11521 aagaaattat ggcgcgactg gcaaaggaag ttcgtcaggc tttttctggt gtccgcgatc
    11581 gtcgtcgccg ctcatttatt ccactggcgc gaaactttga tttcaaaagt actctgcgcg
    11641 ccaacctgca acactggcac ccgcaacacg gcaagttgta tatcgaatcc ccccgcttta
    11701 acagccgcat taaacgccaa agcgagcaat ggcaactggt cttactggtt gatcaaagcg
    11761 gatcgatggt cgattcggtg atccactctg cggtgatggc ggcctgttta tggcagttac
    11821 ccggcattcg tacccatctg gtggcgtttg acacaagcgt cgttgatctc acggcagacg
    11881 ttgccgatcc ggtagagtta ttaatgaaag tgcaattagg cggcggaacc aatatcgcca
    11941 gtgccatgga gtatggtcgg caacttattg aacaacctgc aaaaagcgtc attatcctcg
    12001 tgagcgattt ttatgaaggg ggttcatcat cattgctgac gcaccaagtg aaaaagtgtg
    12061 tccagagcgg catcaaagtg ctgggactgg cagcgctcga tagcacagca acaccttgct
    12121 atgaccgcga tacggcccag gcgctggtta atgtcggcgc acaaatagcc gccatgacac
    12181 cgggcgagct ggcatcatgg cttgcggaga atcttcagtc atgaattcac tacgtccgga
    12241 attattagaa ctgacaccgc aggcactgac ggcgttaagc aatgccggtt ttgttaagcg
    12301 cagccttaag gaactggaaa atggcaacgt cccggaaatc agccatgaga acagcgcttt
    12361 aatcgccacc ttcagtgacg gtgtccgtac ccagctggcg aacggccagg cactgaaaga
    12421 ggctcagtgc acctatgggg ccagcggcat gtgccgtcat cgcgtgatgc tggtgttaag
    12481 ttatcaacga ctttgtgcta ccgcccagcc tacgggaaaa gaagaggaat gggatccggc
    12541 aatctggctg gaagaactgg ctacccttcc cgatgccacc cgcaaacgcg cacaggcgct
    12601 ggtcgctaaa agcatcacca ttgagttgtt ctgtgcgccg ggtgaaattc cctctgcccg
    12661 cttaccgatg agcgatgtgc gtttttattc ccgcagcagt attcgtttcg cccgttgtga
    12721 ttgtattgaa ggcacacttt gcgaacatgt cgtactggca gtacaggcct ttgtccaggc
    12781 aaaagcgcag caagcggaat tgactcattt aatctggcag atgcgcagcg aacacgtcac
    12841 atcatctgac gatccgtttg ccagcgaaga aggcaaaacg tgtcgtcaat atgttcaaca
    12901 actaagccag gcattatggc tgagcggcat cagccagccg cttattcact acgaggccgc
    12961 tttcagccgc gcgcagcaag cagcggaacg ctgcagctgg cgatgggtga gtgaatcgct
    13021 acggcaactg cgcgccagcg ttgatgcctt ccacgcccgc gccagccact atcatgccgg
    13081 agaatgctta cgtcagcttg cggcattaaa cagtcgatta aattgcgcac aagagatggc
    13141 ccggagcgac agtgttggtg aagttcctcc tgtgccgtgg cgcacggtcg ttggctctgg
    13201 cattgccgga gaagcaaagc ttgatcatct gcggctggtg tctttaggta tgcgttgctg
    13261 gcaggatatt gagcattatg gtttacgcat ctggtttacc gatcccgaca ccggcagtat
    13321 tttgcacctt tcgcgcagtt ggccgcgaag tgaacaggaa gactcaccgg cagctacgcg
    13381 tcggctgttt agttttcagg ctggcgcact ggcgggtggg caaattgttt cacaagcagc
    13441 aaaacgcagt gccgatggcg agctgctgtt agctacccgc aaccgcttaa gcagcgttgt
    13501 accgctgtcg cctgatgcct ggcaaatgtt gagcgcgccg ttacgccagc cgggcattgt
    13561 ggctttgcgg gaatatttac gccagcgtcc ccccgcctgc atacggcctc ttaatcaggt
    13621 cgataactta tttattctgc cggtcgctga gtgtatttcg ctcggttggg atagcagccg
    13681 ccagacgctg gatgcgcagg taatcagcgg cgaaggtgaa gataatctgc tgacgttatc
    13741 actgccagcc tcagccagcg cgccttatgc cattgaacgc atggcggcgc ttttgcaaca
    13801 aacagacgac cccgtgtgtc tggtttctgg ctttgtcagt tttgttgatg ggcaattgac
    13861 actggaacca caggtaatga tgacaaagac tcgtgcctgg gcgctggacg cagaaactgc
    13921 gcctgtggtg gtatcactac cttctgccag cgttctgcct gtgccgtcta ctgctcatca
    13981 gttgctgatg cgctgccagg cgttacttat tcaactgctc cataacggct ggcgctatca
    14041 ggaacagagc gctattaatc aggcagagtt gctggcgaat gacctcaccg cggtgggttt
    14101 ttatcggctg gcacatgtgt tggcacaatt tcgtaataca gaaagcgagg cacgggtaga
    14161 agcaatgaat aacggtgttt tgctttgcga acaattattc cccatgcttc agcaacaagg
    14221 atgaaatagt gctttttact aagagttcta ctccagttcc ggactgctca cgccacggta
    14281 ttaggcatat cctatatagc ccctggtgag agtcac
//
LOCUS       DAJTSI010000101        14180 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000101 DAJTSI010000000
VERSION     DAJTSI010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.101"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..441)
                     /locus_tag="OO327_002773"
                     /pseudo
     CDS             complement(1..441)
                     /locus_tag="OO327_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(416..1216)
                     /gene="otsB"
                     /locus_tag="OO327_002774"
     CDS             complement(416..1216)
                     /gene="otsB"
                     /locus_tag="OO327_002774"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCR6019248.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(1383..2369)
                     /gene="araH"
                     /locus_tag="OO327_002775"
     CDS             complement(1383..2369)
                     /gene="araH"
                     /locus_tag="OO327_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR6019249.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2384..3898)
                     /gene="araG"
                     /locus_tag="OO327_002776"
     CDS             complement(2384..3898)
                     /gene="araG"
                     /locus_tag="OO327_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCR6019250.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(3968..4957)
                     /gene="araF"
                     /locus_tag="OO327_002777"
                     /pseudo
     CDS             complement(3968..4957)
                     /gene="araF"
                     /locus_tag="OO327_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            5754..6257
                     /locus_tag="OO327_002778"
     CDS             5754..6257
                     /locus_tag="OO327_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR6019251.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6337..6588)
                     /locus_tag="OO327_002779"
     CDS             complement(6337..6588)
                     /locus_tag="OO327_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR6019252.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(6703..6789)
                     /gene="azuC"
                     /locus_tag="OO327_002780"
     CDS             complement(6703..6789)
                     /gene="azuC"
                     /locus_tag="OO327_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR6019253.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            7052..7375
                     /locus_tag="OO327_002781"
     CDS             7052..7375
                     /locus_tag="OO327_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR6019254.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7546..8043
                     /gene="ftnA"
                     /locus_tag="OO327_002782"
     CDS             7546..8043
                     /gene="ftnA"
                     /locus_tag="OO327_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR6019255.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(8080..8319)
                     /locus_tag="OO327_002783"
     CDS             complement(8080..8319)
                     /locus_tag="OO327_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR6019256.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            8509..9720
                     /gene="tyrP"
                     /locus_tag="OO327_002784"
     CDS             8509..9720
                     /gene="tyrP"
                     /locus_tag="OO327_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR6019257.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(9782..10447)
                     /gene="yecA"
                     /locus_tag="OO327_002785"
     CDS             complement(9782..10447)
                     /gene="yecA"
                     /locus_tag="OO327_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR6019258.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(10643..10729)
                     /locus_tag="OO327_002786"
     tRNA            complement(10643..10729)
                     /locus_tag="OO327_002786"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10693..10695),aa:Leu,seq:taa)
     gene            complement(10742..10815)
                     /locus_tag="OO327_002787"
     tRNA            complement(10742..10815)
                     /locus_tag="OO327_002787"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10781..10783),aa:Cys,seq:gca)
     gene            complement(10870..10945)
                     /locus_tag="OO327_002788"
     tRNA            complement(10870..10945)
                     /locus_tag="OO327_002788"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10910..10912),aa:Gly,seq:gcc)
     gene            complement(11097..11645)
                     /gene="pgsA"
                     /locus_tag="OO327_002789"
     CDS             complement(11097..11645)
                     /gene="pgsA"
                     /locus_tag="OO327_002789"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR6019259.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(11702..13534)
                     /gene="uvrC"
                     /locus_tag="OO327_002790"
     CDS             complement(11702..13534)
                     /gene="uvrC"
                     /locus_tag="OO327_002790"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR6019260.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(13531..>14180)
                     /gene="uvrY"
                     /locus_tag="OO327_002791"
     CDS             complement(13531..>14180)
                     /gene="uvrY"
                     /locus_tag="OO327_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR6019261.1"
                     /translation="VLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCRTNA
                     VDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYLSKG
                     AAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLMITK
                     GQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ"
BASE COUNT         3550 a         3677 c         3278 g         3675 t
ORIGIN      
        1 caatgcgatt attcacccca cgtttgcgta attcatgcgc aaagggcaac aggtgataat
       61 cgtggatcca gataatgtca tcgtcttgca acagcggcag taatttatct gccagcaacg
      121 catttacgcg tagatagccg tcccaggcag gacgctgaaa ttgcaccaga tcgagccgat
      181 aatgaaaagc gggccagaga acggcattgg agaattggtt gtagtattcg tcaaggtcct
      241 gttcgctgag gttaaaagag gcccacgtaa tgttaccttt tttagcggct gatcctcatt
      301 ccctgtttca ccactccagc caaaccacag tccgcctgcg gctttcagtg cccccagtat
      361 gccaacggca aggccaccgg cactggcggc gtgctcgtct ggtggtgcaa tccggttaga
      421 tactacgact aaacgactca tagtcatcac tcctgttatt ttctcttttt tgttgtaatg
      481 cggcggttat catttcaagc cagctccaga catccggcac acccgccagt cgccatgatg
      541 cctgagttgc acctgtgcca atttttactg acattccgcc cagtcggtta acgactgcga
      601 agccagattc atcggttaaa tcatcgccca gaaatacggg cgttcgcccg ataaaaggag
      661 cttcctgcat aaaggctgca atcgcttcac ctttactggt acctctcggt ttgatctcga
      721 caacacactt tccctgctgt aacgccattt gtggccagat ctgagtaata cgttgcgcta
      781 atgtcattaa tgcgtcttca tgctgcggag cctgacgata atgcagcgca aaagccatcc
      841 ctttcgcctc cagctccgcg ccgggatact gagcgatgac cgtatgcagt tgcacgctaa
      901 tatcacgcgc aatcgcatcc ggcagatgaa cgatatgtgt tttaccattg atgtcacggc
      961 gctccgcccc atgcacgccc gctaacggga agcgataagg ttttgccagt gcgtcaagct
     1021 ccaccattga gcgccctgat atcaatgcca atgcaccatc acttgcggtt gccagtagct
     1081 gtagtccttg cagaatattg tcaggcacga cgacctgatc gggatgcggt ttgatttccg
     1141 ccagcgttcc atcaagatca aaaaaccagg catatttcgc ggatagttca ggggtttcgg
     1201 ttaacggttc tgtcacccgg ctctcctcct tctcttttct tattgtgttg cgcagaccgc
     1261 gctttacaga catgtaagta tagacagtgt gacggaggtc gccatttgca acgaagaaca
     1321 gccaaccgtt gtggcggctg gctgtggtgg gaaaaaacgt taagttgttg tggaaaaaag
     1381 catcagacag tgcgcttcgc tttttgcttg taacggtcga agatcaccgc tgccagcagg
     1441 attaagccgc gaaccacgta ctgcgcgaaa ggagaaatat taagcaggtt catggcgttt
     1501 tccacggtgc ctaaaattaa gatacccgcc accacatatg agatttttcc gatgccacct
     1561 ttcagagaaa cgccacctaa aacgcaggcg gagataacaa taagctcata accaatcgac
     1621 gtcattggct gcccactagt catacgtgaa gccagaataa ttccggctat cgcagatacc
     1681 aggccagaga gaacaaagat aataattttg gtgcgaacaa ccggtacacc cgccagacgc
     1741 gcagcctctt cgttcccgcc aatcgccagg gtattacgac caaaggtggt tttattcagc
     1801 agcaaaccaa agataatcag acacgcgacg gtgagccaga ttggcgcagg cagaccgaac
     1861 cagttagcgt aaccaagggc aaagaagctt tcatcttcga taccgaccgc tttaccgtct
     1921 gaaatgatgt acgccagacc tcgaacaatc tgcatcgttg ccagtgtcgt gatcagagca
     1981 tttattttca gtttggcgat aacaaagcca ttgaccaggc cacagagaac gcccagcaac
     2041 aaacccgctg ccacgccaat ccacaggctt tcagtcaggt tgataaccac cgccgtggtg
     2101 acacccgcac aggcaattac ggaggcgaca gaaaggtcaa agtcaccgga agcaaggcag
     2161 aacaacatgc cacacgccac catccccgac atggaaattg ccaggcccaa ccctttcata
     2221 ttaatgaagg tggcaaaatt tgggacaaaa atggcacagg cgataaagag caccgcaaac
     2281 accaccagca tgccgtactg atcccagata cgcccgaagc tgaatgacga cttaggtgcg
     2341 ccagaccccg atgtagaaac agaagacatc atactctcct tactcaggca actgcctggc
     2401 tgactttagg catcgcaagg ctcagtgcct gacgctcatc tgcctgctcg tgtaacaatt
     2461 caccggcgat ttcgccttcc cgcatcacca caatccggtc ggcaacgccg aggacttcag
     2521 gtaagtcgct ggaggcaaac agcaccgcca cgccctgcgc cgccagcgca taaattacgt
     2581 tatatatttc gtgcttagcg ccaacatcaa tgccgcgcgt aggttcatcc agcaaaatga
     2641 ccttcatctc ttccgataac cagcggccca gaatggcttt ttgctgattt ccgcctgaga
     2701 gattcatgat cagttgctcc gcgcccggcg ttttgatgtt gagcgaacga atgtgctgat
     2761 cggcattgtt ttcttcccaa ctgttgttga ttacacaacc gccgagcaca tgtttgcgtc
     2821 tggcactgat gttgatattg tcgcgaacgg agtgcacggg aataatgcct tccgctttgc
     2881 gagcttccgg gcagagcatc atgcctgcgg caatggcgtg gctcggttta cgaatatcga
     2941 tcggctgttg gtcgatataa acctgaccgg cggtgatttg cgtcccgcca aacaagcctt
     3001 tcattaattc gctacgcccc gcccctacca gaccaaacag cccaacaatt tcaccactgc
     3061 gaaccgccag acttattggc gtacgcacgc ctagtgcttt cacagcatca agacgcaggc
     3121 gctcctcgcc ataactacga ggttgccagc cgtagatatc gccaatgtcg cgcccgacca
     3181 tcgcctgcac cagcgcgtcg tggtcaacct gctgcatatc ggtaaaggtt ttgacataac
     3241 gtccatcttt aaagacagtg atggcatcgc tgagggcaaa tatttcttcc atacggtgag
     3301 aaacgtataa gatcacccgc ccctcttttc gcagttcacg aataacgcgg aaaagattgc
     3361 cgatttcgcg ggcagaaagg gagctggttg gctcatcaaa ggcgataatt ttagcgttac
     3421 gcgccagcgc tttggcgatt tcaaccatct gccactgacc aatggcgaga tatttcagcg
     3481 gcgtgtccgg gtcaatatcc ataccaagat gtttaagttg taaacccgcc tcataattca
     3541 gcaatgagcg attcacaatg ccgcctttat gcggcagctg gccgagatag atgttttccg
     3601 cgacggtcat ttccggcacg agatgcagtt cctggtaaat aatcgccaca cccgcgttaa
     3661 gtgctgcggt cgtgtcggaa aaggacattt cctgcccatt aatcactaca gaacccgtgg
     3721 ttggcgcata gttgccgctg aggattttta agagagttga ttttcctgcg ccattttcac
     3781 ccatcaacgc atgaacctga ccggcatagc agtcaaaact aatatccgtc agcgccttaa
     3841 cgccgggaaa cgttttaccg atgccgcgaa atgagagata cggggtagac tgttgcataa
     3901 cgtctccgtg aatcactggt cgttcacacc ccctctgcat catgcagagg ggaatttttc
     3961 cggcaaatta cttaccgcct aaaccttttt tctccagttc ttctttaaag ttgtcacgcg
     4021 tgatcagtac cacgtctgta acttcggtaa attttggcgt ttcaacgtct tttgctaccc
     4081 agttgtaaag catttcgctg gatttatagc catgtacgtc cgggcttggc agcagggaac
     4141 cgtagaagcc ggttgcctgt gctttagaca gttcgcttac tgcatccaca ccgttaatac
     4201 caataccgat gatatcggct gctttaaagc cctgaccttc ggttgcacgt acgccgccaa
     4261 gtacggtgct gtcgttcata ccgacgatca gccaatgttt aacttccgga tgttgaacca
     4321 gcattgagtt ggcagcgtca aatgcccccg ggatgtcgtt agatttggta ggtacctgat
     4381 aaatttgttt ttccgggaat cctgcagctt tcagtgcgtc catagaaccc gttgtacggc
     4441 ggcgggcggt atccagttcg ttggcggtaa tcgccatcac tgcgctttct ttgacatccc
     4501 agccacgttt ctgcatctct ttatacagtt cctagccctg acgttcgcca attttagtcg
     4561 ccgccatcat caccagagga acggtatcca ttggcttacc tttggcgtta acaaactggt
     4621 catccactgc aatgactttc atatcgtagc cacgcgcttt cgcgacgatg gcagagccga
     4681 gtttcgggtc cggagtacaa ataacgaaac cttttgcgcc actggctgcc aggctgtcga
     4741 tcgcgttcaa tgttttttcg ccatccggca cggcaatctt aataacctca aaccctaaat
     4801 ccttcccggc tttatcggca aacttccatt cggtctggaa ccacggctct tccggttgct
     4861 tcaccagaaa accgagcttc aggttctccg ccatagcgga ttgtgacata acggctgcca
     4921 gaccaatggc tgccagggct ttagtaaatt tgtgcatggt tctctccagc tttagtgtcg
     4981 ttttgtgtag ggcaaaaacg aatgacattc gttaaattaa tcggaaaaca aagcattacc
     5041 ttttatctaa aagataagtg accgtgttga catagtttta gcgagaaatt aattctccat
     5101 aggagagcaa tatcacatcg cagaattaca gtgagaacgt gcataaattt agcgggaaaa
     5161 gacataagga aaagccaatt tgtcagacaa attgttgaat gcacagcaga ttaatccata
     5221 agattagcct ggaaatcctt gttgtcttcg gtacccatgc gggatgtctt ctttttaacc
     5281 agtcaatagg ccgcattacc tggcgttgag tttttgaaat ggtgtaataa ccacaactca
     5341 aagatgtgga aaatgcgcgt cattcatttc gccattaatt atcactgtgc tcattaatta
     5401 acagaacacg tataatgaga gccatctcgc aaaaatgaaa aaacgtttta taaaatcatc
     5461 acttcatcat gaattcaaat tcatcgatta atatcaacaa gatacaaaaa gcactatcat
     5521 taaaattcat tgcatttaca ttgatttcac caatgaaatg taaaaatata taaacttgat
     5581 gatttaagca ttttcttata accgttcaga cgttattctt atttcagatc atcgtcagaa
     5641 ttgactccac gatcacattt cggaccggca gaaaggaatt attctgcaaa cagtaattat
     5701 ggtgttttga tttatcttgc acctctccac ttctggatat aaggatatta ggtatggcaa
     5761 ccgctggaat gcttctcaaa ctcaactctc aaatgaaccg cgagttttac gcatccaatc
     5821 tctaccttca cctgagtaac tggtgttctg aacagagtct gaacggcacc gccactttcc
     5881 ttcgcgccca ggcacagagt aatgtgaccc aaatgatgcg catgtttaac tttatgaaga
     5941 gtgtcggcgc tacccccatc gttaaagcca ttgatgttcc aggtgaaaaa ctgaactctc
     6001 tggaagaact gttccaaaaa acgatggaag aatacgagca acgttcaagc actctggcgc
     6061 agttagccga tgaagcgaaa gaactgaatg atgattcaac cgtcaatttc ctgcgcgatc
     6121 tggaaaaaga acagcagcat gatggcctgt tgctgcaaac cattcttgat gaagtgcgca
     6181 gtgcgaaact tgcgggtatg tgccctgtgc agaccgacca acatgttctg aatgtcgtgt
     6241 cacaccagct gcattgatca tacatcggcg ctaatacatt gcgccgatga agtttttgag
     6301 aaaccgctgc ctcatctgtt tgaagcagcg gtttttttaa tgggattcac cctgtggggt
     6361 aaacttgagt tcaatcagcg cgatggcttt ttggattgcc cgcatggtga ccgggtctgc
     6421 ggcagcggga tgatgagtaa agtcgatatt cttcagctga ctggacattt tttcacgaac
     6481 ttcaacgggc gcgattacat cgagaacatc cagaatttgt ttgataacca actggcaagc
     6541 taccacatca gaaaccagtt cctgatcggc attcagcggc tgggacatcg taaactcctg
     6601 atagcatttt gaaagccgtt atagtagcgg cttcacatct tcagcgatag tcacatccac
     6661 cgtcatcagg acacaaaaaa acctgccgga gcaggttttt tgttatcgga acatattgcc
     6721 tggcggtacg tctttgaacg tcttgcaata gttattgaac atacttttca ggattttgcg
     6781 cagtttcatc gcggcactcc gaccatttgt tatacaggtg ttattgtctt tgcgcttata
     6841 atatgacaac catcacaaaa atcaatcttt atgtgataca aatcacataa ataccccttt
     6901 aatgttataa aaatgataat caaaaaacag cccccctatt tctgacacct acagatggca
     6961 agaaatagcg cctgccaggc gtcttttccg gccattgtcg cagcgctgta acgcgtaaaa
     7021 tagtgctttc tcttactctt ctggctggac catgagactt ctgattctga ctctttcatt
     7081 aataacgctg gcggggtgta cggttactcg ccaggcccat gtgagcgaag ttgacgcggc
     7141 aacaggtatt gtacggttgg tttatgacca ggcttttttg cagcatgccc ataccgatcg
     7201 ttatgtcagt cgcgggatcg ccgatcgcgc atgccagcag gaaggctata cccacgcgat
     7261 cccctttggt cagccagtag gcaactgtag cctttttgcg ggttccttat gtttgaatac
     7321 ggaattcact ttgtcatatc agtgccatca ttccgccttc cctgtctttc tctaaatatt
     7381 ttaatcagcg aggggatctt cgctgattaa agaaatagcg gtaatgcgtt ttattcccat
     7441 tcgtattttt aataattgaa gtttatattt taccttttgc aaataataaa ataacaaatt
     7501 atagtgacgc cacagcaaca aatataacct ttgtggagca ctatcatgct gaaaccagaa
     7561 atgattgaaa aacttaatga gcagatgaac ctggaactgt actcttcact gctttatcag
     7621 caaatgagcg cctggtgcag ctatcatacc ttcgaaggtg ctgccgcgtt cctgcgccgt
     7681 cacgcccagg aagagatgac gcatatgcag cgtctgtttg attacctgac tgataccggc
     7741 aatttaccgc gtattaatac cgttgaatct ccgtttgctg aatattcctc acttgatgaa
     7801 ttattccagg aaacctataa acacgaacaa ttaatcaccc agaaaattaa cgaactggct
     7861 catgctgcaa tgaccaatca ggactaccca acatttaatt tcctgcagtg gtatgtttct
     7921 gagcagcatg aagaagagaa actgttcaaa tcgattattg ataaattaag cctggcaggc
     7981 aaaagcggcg aaggtctgta ttttatcgac aaagaactct ctaccctcga cacacaaaac
     8041 taatgctctc cggcggcagg tttctgccgc cgtttcaaat cagtgacggc aaatcttact
     8101 ctgatcggtc gaaaaacctt cttctgaagg tataaatttg ccttttgctg ccagaaacgc
     8161 taccagctct cccgctgtca ccccttctgc cgagcacgtg tgaaaacgtg cctgttcacc
     8221 aaaacgcgct ttaatcgcag cttccagact ggcatgcgta tattgctcgc ctgattcaat
     8281 catcatattt aacacttcat gaccgtgaat agagtccatc gttcctcctc aaaaaaagcc
     8341 tagcgtagcg attaccgctt atgaagactt tgcgccagcg caggactgaa tgcttttatt
     8401 gtacatttat atttacacaa tatgtaacgt cggtttgacg aagcagccgt tatgccttaa
     8461 cctgcgccgc agatatcact cataaagatc gtcaggacag aagaaagcgt gaaaaacaga
     8521 accctgggaa gtgtttttat cgtggcggga accacaattg gcgcaggcat gctggcaatg
     8581 ccgctggctg cggccggtgt tggttttagc gttacgttaa tcttgttgat tgggctttgg
     8641 gcgttgatgt gctacacggc gctattactg ctggaggtgt accagcatgt tccggcagat
     8701 accggtctgg gcacgctggc aaaacgctat ctgggacgct acggtcaatg gctgacgggc
     8761 ttcagtatga tgttcttaat gtatgctctg actgcagcat acatcagcgg tgccggtgaa
     8821 ttgttggcct ccagcatcag cgactggaca ggtatttcaa tgtcggcaac cgctggcgtg
     8881 ctgttgttca cttttgttgc cggtggcgtg gtttgtgttg gaacatcgct ggtcgattta
     8941 tttaaccgtt ttctgttcag cgccaaaatt atttttctgg tggtgatgct ggtattgctg
     9001 ctaccgcata ttcacaaagt gaatctttta accctgccgt tgcaacaggg gctggcactg
     9061 tctgcaatcc cggtgatttt tacgtcgttt ggttttcacg gtagcgtgcc gagtattgtc
     9121 agctatatgg atggcaacat tcgtaagcta cgctgggtgt ttataatcgg tagtgccatc
     9181 cccctggtgg catatatttt ctggcaggtg gcgacgcttg gcagcattga ttcaacaacc
     9241 tttatgggat tgctggctaa tcatgctgga ttaaacgggc tgttacaggc gttacgcgaa
     9301 atggtggcct ctccgcatgt tgagctggcg gtgcatttat ttgctgattt agccctcgcc
     9361 acgtcatttc tcggcgttgc gttaggctta tttgattatc tggctgattt gtttcagcgt
     9421 tcaaataccg ttggtggacg attgcaaact ggtgcaatta cctttctgcc gccgttggcg
     9481 tttgcactgt tttatccacg aggatttgtg atggcgctgg gttacgccgg tgtggcgctg
     9541 gcggtactgg cattgattat cccttcacta ttgacctggc aaagcagaaa gcacaatcct
     9601 caggcgggtt accgggtcaa aggtggtcgt ccggcgctgg tggtggtgtt tctctgtggt
     9661 attgctgtga ttggcgtgca atttttgatt gcggcagggt tgttaccaga agtggggtga
     9721 tcagatagcc tcaaattcct tattgggtgc cagaattaac gctgacaccc aatgtgacct
     9781 cttaatgcag gcagcactgc ttaaatttct taccactgcc gcacggacaa ggatcgttac
     9841 gccccggttt ctcttctgct ttgatcggtt gctgaacagc tttttcctgc ggatgcgcca
     9901 tccagtatgc atgtaaatca agcgccgcca gtcgaatggc atctacgctc tcttcaaacg
     9961 cttctggcga cattttctct acccgctcga agttttcctc agtaccgtgc agcgcaatcg
    10021 cctccagcgc tggttttaaa gaatcgggca acgttgacca gtcagaaagt gccacgcccc
    10081 gcatatagcc aaagcaccac tcctcaacaa tcgtcagctc gctgccatca acttctcgca
    10141 agccgaataa cggctcaaac tgctccggga attcgttcag acgctctgcg gtatcggcca
    10201 tatgttgaaa agccaggttc ataaagcgcg tcatctcttt ctctgacgcc caacgcggca
    10261 catagtcagc cccaccccac acggcaacca gccactgttc tggttcaatc tcttgcggag
    10321 aactcaacac cgccgtcaat aaaccgtcca gctccgccac atcaaggatg gcgtggtcag
    10381 tgttgtattt ggtcagaata tcgtccagcc attccaactc actttcgttt aacggtcccg
    10441 ttttcatacg cttttccttg tggatctcaa ctcgccagca cctatcttac atgccggtcc
    10501 gtatcagaga tactttttga gtggctttgc cggtgattaa aaattaagga gggtgtaacg
    10561 acaagttgca ggcacaaaaa aaccacccga aggtggtttc acgacactgc ttattacttt
    10621 gattttattc ttatctttcc catggtaccc ggagcgggac ttgaacccgc acagcgcgaa
    10681 cgccgaggga ttttaaatcc cttgtgtcta ccgattccac catccgggct cgggaagaaa
    10741 gtggaggcgc gttccggagt cgaaccggac tagacggatt tgcaatccgc tacataaccg
    10801 ctttgttaac gcgccaaatt cttcaggcct ttcagccaga catccgcttg acgccgatgt
    10861 cttttaaact ggagcgggaa acgagactcg aactcgagac ctcgaccttg gcaaggtcgt
    10921 gctctaccaa ctgagctatt cccgcattca tcaagcaatc agttaatcac ttgattttat
    10981 tatcgtctgg caatcagtgc cgccgttcga tgcgttgcat tctacttacc tggcgcgatg
    11041 agtcaacgat atttttcacc acttttgatc gtttgctgaa aattacgccg aaacgatcac
    11101 tgatcaagca aatctgcacg cgcagcgctc aaatattgca acattgacca cagagtcagt
    11161 accgcagcca caaagaaaag tgcaataccg acgtactcaa cccaaatgtt cggacgccac
    11221 agcagccagg ccaacgccac catctgggca gtggttttca ctttcccaat ccaggagacg
    11281 gccacgctac tgcgtttacc caactccgcc atccattcgc gtagcgcaga aataataatt
    11341 tcacgggcga tcatcgttgc cgccggtaat gtcacccacc agctgtgata atgctcggtt
    11401 accagcacca tggcgatagc cacgagaact ttatctgcca cagggtcaag gaaagcacca
    11461 aaccgggtac tctggttcca gcggcgtgcc agaaaaccat cgaaccagtc agtcaccgcc
    11521 gcgacgcaga aaatgagcgc ggcggcaaac ggcgaccagg tgacaggcag ataaaagacc
    11581 aatacaaaga atgggataag gatgacacgg aacagtgtaa gcaacgtagg gatattaaat
    11641 tgcataatga cgggtaacta tctgttgtca gtaagattac ccctatgttg ctacagagac
    11701 atcaatgttt caacgaccag aagatctttt ctgccagacc ttgcgaaata cccggcactt
    11761 ttgcaatttc ctcgacgctg gcgttacgta aaccttgcaa accgcccata tatttcaaca
    11821 acatttgccg acgttttggc ccgacgcctt caatggtttc cagggaactg gtatttttga
    11881 ccttcgcccg ttttttacgg tgcccgccaa tcgcgtgatc atgtgattca tcgcgaatat
    11941 gctggataac atgcagcgcg ggtgaatctg gcggcaaact aaatccctca ccttccggct
    12001 caaagaacag cgtttccagt ccagccttac gatctgctcc tttggcaacg ccaagtagca
    12061 gcggatgatt tttatcccat gagacatcca gttcggcgaa gacatttttc gcctgcgcaa
    12121 gctggccttt gccgccgtcg ataaggatca catccgggat cttactgtcg tcaatggctt
    12181 taccataacg ccgacgcagc acctgattca tcgccgcata atcatcgccc ggcgtgatgc
    12241 ctgtaatgtt atagcgccga tactccgcac gcagcgggcc gttagcatca aacaccacac
    12301 aggaagcgac ggtttgttcg cccatggtat ggctgatgtc aaagcactcc atccgcttca
    12361 cttccggcaa tttcaacacg ctggcaagcg cggtcagtcg ctggtgaacg gtagattgct
    12421 gcgaaagttt gctggttaag gccgtcgccg cattggtgcg cgcgagtttc agataacgcg
    12481 ccctatcgcc gcgaggtttg gtttgaacat taatcttgcg tcccgccagt tctgaaaggg
    12541 aatcggcgag cagcgtttta tcgctaagat taaaatcgag caggatctca cccggtaagg
    12601 tgcgcatctg gctgccttgt aaatagaact gacctacgaa ggtttctacc acctcgctca
    12661 gttccgtacc gccaggcact ttcgggaaat agctgcggct gccgagcact ttgccctgac
    12721 gaatgaacaa tacgtggaca caagccatgc ccgcatcgaa cgccacacca ataacgtcaa
    12781 ggtcgtcgcc ggtattggaa acaaactgtt tttcggtgac gcgtcgcacc gcctgaattt
    12841 ggtcgcgaat acatgccgct tcttcaaact ccagattctg gctggcagtt tccatacggc
    12901 taatgagttg cgtaagcacc tgatcatctt tgccagacaa aaacaggcgc acatactcga
    12961 cctgctgagc gtattcttct tcactcacca gtccttcaac gcacggtccc agacagcgcc
    13021 ctatctggta ttgcagacac ggacgcgagc gattgcgata aacactattt tcgcactggc
    13081 gaatggggaa aatcttttgc agtagcgcca gtgtttcacg tacggcatag ccattcggga
    13141 acgggccgaa atattcacct ttggcatgct ttgcaccacg atgcatcgcc agacgcgggt
    13201 gggtatcgcc actcaggaag ataaaaggat aagatttatc atcgcgtagc aaaacgttgt
    13261 aacgcggctg atagagtttg atgtagttgt gttccagcaa cagcgcttcg gtttctgtat
    13321 gcgtaaccgt tacatcaatt tgctggatct gggcgaccag cgcttcggtt ttgcgcgaag
    13381 cgaggttgct gcggaaatag ctggaaagcc gttttttcag gtctttcgct ttgccgacat
    13441 agataaccgt accaccagca tcgtacatgc gataaacgcc tggctggctg gttacggttt
    13501 ttaaaaacgc ttttgcgtca aactgatcac tcactgactt gataatgtct ccgcattaca
    13561 cagaccatgg cgaattgcca ggtgagtcag ctcaacatcg ccatgaatgt ttagtttact
    13621 gaacatacga tagcggtagc tgttcaccgt tttcggactg agattgagct gttctgagat
    13681 ctcattgacc ttctggccct tagtgatcat cagcataatc tgcaattcac gttcagacaa
    13741 actggcaaat gggctttctg ttttttctgg ttcgatctgg cttaacgcca tttgttgagc
    13801 gatatcagaa gcaatgtaac gctgccctga atagacagaa cgaatcgcac tcacgacttc
    13861 ctgcggagcc gcgcctttgc tgaggtagcc agcagcacct gcctgcatga ctttcgctgg
    13921 taaagggttt tctgtatgga ccgtaagcat gatgatttta acatcagctg tggaacgcgc
    13981 gattttacgc gtcgcctcaa gaccgccaat gcccggcata ctcatgtcca ttagcaccac
    14041 gtcaacggca tttgtccggc accacttaac ggcgtcttca ccgcacgatg cctctccgac
    14101 gacttttata cccttaatat cttccagaat gcgtcgtatc cctgcgcgca ccagttcgtg
    14161 gtcatcaaca agtagaacgt
//
LOCUS       DAJTSI010000102        14022 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000102 DAJTSI010000000
VERSION     DAJTSI010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14022
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.102"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            558..1349
                     /locus_tag="OO327_002792"
     CDS             558..1349
                     /locus_tag="OO327_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019262.1"
                     /translation="MTTRITFSMTSDETLRIVDEYCHTHKLSRSKVINALLSATAPVL
                     NDINCYYQLAGELQSRLLNGVYQRDLPRKRNVVSAEKYCLEIWENKLFTRRILEFDSS
                     NGVLYALKHKRHYRRDKMIGRVESRCIKDICEYQMQLSGEKTKYACFIYIERTIYNHD
                     NPSDKIPVKAAVGNAVILLAKDVIYNEYFFDLRQSFFVSVTDLMVSGAKGIPETQTYP
                     DVYCWIPLFSINSGVLITPVYKIDPLKPVTVKKPDKITVVCNYRE"
     gene            1735..1941
                     /locus_tag="OO327_002793"
     CDS             1735..1941
                     /locus_tag="OO327_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR6019263.1"
                     /translation="MNTPVSLMDDQMVDMSFITQLTGLTDKWFYKLIKHGAFPPPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            2027..2599
                     /locus_tag="OO327_002794"
     CDS             2027..2599
                     /locus_tag="OO327_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019264.1"
                     /translation="MQQLENRCDLLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGSVISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(2601..2885)
                     /locus_tag="OO327_002795"
     CDS             complement(2601..2885)
                     /locus_tag="OO327_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019265.1"
                     /translation="MVVLNTPGKYQYDLRNHLLVNLFPIALDAEITTSNVNKHQLHRS
                     NIPAALSGNSGITDYVPFSAERNAAMVIWRTAIDSRPAVCNDEEGTPGTL"
     gene            2856..2999
                     /locus_tag="OO327_002796"
                     /pseudo
     CDS             2856..2999
                     /locus_tag="OO327_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            4168..5187
                     /locus_tag="OO327_002797"
     CDS             4168..5187
                     /locus_tag="OO327_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6019266.1"
                     /translation="MNNEQMLIKCRDPRKCRFYAEYLNHKYPNEFPHFAPDNPQNCIR
                     NVNLFLKEKYCNVSPPPFGTFGTTEEEALNNEINELEIIAGQAILPEESFLWLKTDEL
                     ATFFTWTTIYLSKEDHTKFPKINHNSSGIKNGIEVRKIRTVTDETLNTYKKSNLPDNP
                     SSHNERIKVIISYFDSCHSLINKNAKSIYLEELRQAWLKKKSACQKFKWLLPDDDEMC
                     QWFWTRLKQKQDEKHPVHNPVTRWFIPSSTKEYYLASLVAFALWKEAPDTIELFRTRI
                     NHAWHQKKQKDKYRSDGKKAINIHIRSNVKEMLDELRTVYGLRTGEFFEMLITEKYLE
                     HKRRK"
     gene            5297..6169
                     /locus_tag="OO327_002798"
     CDS             5297..6169
                     /locus_tag="OO327_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCR6019267.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            6503..9625
                     /gene="ag43"
                     /locus_tag="OO327_002799"
     CDS             6503..9625
                     /gene="ag43"
                     /locus_tag="OO327_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCR6019268.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARAQGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTGGQ
                     WIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVH
                     GGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTIN
                     KNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRDGW
                     QVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAFSV
                     VEGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADS
                     GAAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLA
                     GTTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGALTGNGSVEKSGSGTLTVSNTTL
                     TQKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNN
                     ADRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFIQGNKL
                     QAGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVSG
                     ENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSVTAGVY
                     GAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLIHNASGLWADIVAQGTRHSMKASS
                     DNNDFRVRGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNASYVKFGHG
                     SAQHVRAGFRLGSHHDMNFGKGTSSRDTLRGSAKHSVRELPVNWWVQPSVIRTFSSRG
                     DMSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARVRENITLGVQASYAHSINGSSAEGY
                     NSQATLNVTF"
     gene            9746..12262
                     /locus_tag="OO327_002800"
     CDS             9746..12262
                     /locus_tag="OO327_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019269.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALTSTASALAGFILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRTIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAISQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPAPEWYCSSLCHETETLCQDIYERSYTGFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLTVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKVMKGTLVTSLALVAVTTSPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGKMVNRLEEAFPPELPA"
     gene            12338..12793
                     /locus_tag="OO327_002801"
     CDS             12338..12793
                     /locus_tag="OO327_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019270.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNGEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKNVN"
     gene            complement(12912..13123)
                     /locus_tag="OO327_002802"
                     /pseudo
     CDS             complement(12912..13123)
                     /locus_tag="OO327_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13204..14022
                     /locus_tag="OO327_002803"
     CDS             13204..14022
                     /locus_tag="OO327_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR6019271.1"
                     /translation="MQLVSRFGYANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     TYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
BASE COUNT         3772 a         3470 c         3463 g         3317 t
ORIGIN      
        1 aataacagac attacttaac aaagaacatt acagtcagtg tgtacattac agatttatat
       61 tcaggtgttg cgggactaca ttacagcaga gaggggtaag gacagagtta caagtactac
      121 tagagagaat atcactgcac agatacttct atgagtatat ctttatgaga gatccgtcta
      181 caggattggg ttataaggtg tatttacaag gtataatcac aggatcattt acatgaccat
      241 acttctgtaa ataatactaa ttaaccttaa ttattttttt atggtacatg aactacctgt
      301 agctgtaacg ataaacaaat cacccaaaat gaaaaatact ggttatcaca gttcagagaa
      361 ctcgtatgag tgtccccctg agcaatgctt cattttctgt gattctgtgt gatagagtta
      421 tactgagtac agtggcagta acactgcttt ccatcacttg aggataaaat tcattaaaaa
      481 attaattgca tgaattaaca ggttttttta tgaaatgtta tgcatctcaa ccaatatgat
      541 taacagaaaa cttcatcatg acaacacgta taacgttcag tatgaccagt gatgagacgc
      601 tgcgaattgt ggatgagtac tgtcataccc acaaactttc gcgctctaag gtaatcaacg
      661 cattactcag cgccacagca cctgtcctta atgatattaa ctgttattat caacttgcag
      721 gcgaactgca gtcacgctta ttaaatggag tgtatcaacg ggatcttcct cgcaaacgca
      781 atgtcgtcag tgcagagaag tattgtctgg aaatctggga aaacaaactt ttcaccagaa
      841 gaatcctgga gtttgacagt tcaaatggtg ttctgtacgc gctgaaacat aaaagacact
      901 atcgccggga taagatgatt ggaagggttg aaagtcggtg tatcaaagac atttgcgaat
      961 accagatgca gctatccggg gagaagacga aatatgcctg ttttatttat atcgaaagaa
     1021 caatatacaa tcacgataat ccatctgata aaatacctgt aaaagcggct gttggtaatg
     1081 cagtcatcct gctggcaaag gatgtgatat ataacgagta cttttttgat ttgcggcagt
     1141 cattttttgt gagcgttaca gatctgatgg tctctggcgc aaaaggtatt ccggagacac
     1201 agacatatcc ggacgtttat tgctggatcc ctcttttttc cataaattcc ggcgtgctca
     1261 ttacccctgt atataaaatt gaccctctga aacccgtaac ggtaaaaaaa ccggataaga
     1321 tcactgttgt ttgtaattac agagaataat ttcagccttc taccttcacc gcgacagtgt
     1381 ttacctctgc tttatcttcc gttcagtaaa tacaaaccac cgctctggca ggtacagggc
     1441 gtggtttatc tccgatactc ccctgtgcag taataatcat cactgaaaat actgatcccg
     1501 tcacggcaaa acgccatccc atattccggc aacacaaaat catcttccgt ttataccgga
     1561 ttcactgaca aaacagacaa cctgtattgc ctgtacacaa cagtttctct cctggcagga
     1621 atattcccct actcattttt tacgtttctc cccggttgtt atgccggtga aggtggtgcg
     1681 tcgttttcat caccaacgcc ggttgagatt aacaacatcc ggaggaacat tctcatgaat
     1741 accccagttt cactgatgga tgaccagatg gtcgacatgt cgtttatcac tcagctgacc
     1801 ggcctgaccg ataagtggtt ttataaactc atcaagcatg gagcctttcc gccccccatc
     1861 aaactgggcc gtagctcccg ctggctgaaa agcgaagtgg aagcctggct gcaggcgcgt
     1921 attgcacagt cccgtccgta attcctgacc gttatccgtt ccaccgcagc aaaggcatga
     1981 tccggccccc ggaggcattt tgctgcgcgc taaaggagag aatattatgc aacaacttga
     2041 aaacaggtgt gacctgctgc tcatccagca tcagaaatgg atgacaagcg tgacgaggct
     2101 tatcgtcgct catggaatgg ggtctccgca tcttcatggt tatcaccgtc tgaccctggc
     2161 acactttttt ctgccggaaa aaggctccgt catttcggtg gccccccagg gactctatca
     2221 ggtcgtcaac ccgggaacac caccgttcat tcctgccatt caggaagggt taatgaccag
     2281 cattcagacc catgaaatca tgctgcttac acattttaat ctgggcggcg ttttactcag
     2341 tgagctacat cgtctgggtg aaaaccggct ggcaaacagg ctgaacagcc tgctccggcg
     2401 tttcgacgat cgtgatctct accacacgct gatatggctg tgctggtatg atctgatgtg
     2461 tgcccactcc atgcagccct ggactgaaga gttaaagcat aaatcacatg cagagcttga
     2521 aaactgggct gtggcgcgca aacgggaaaa gcgcgagctg gaactgatga ttgacgagta
     2581 cctgttatac gcctgctgat tcataacgtt cccggggttc cttcctcatc gttacacaca
     2641 gccggacggc tatctatggc tgtccgccaa atgaccatcg ccgcatttcg ttccgctgaa
     2701 aacggtacgt agtcagtgat accggaattt ccggagaggg ccgcaggtat attgcttctg
     2761 tgcaattgat gcttatttac attggatgta gttatttctg catccaatgc tatcggaaag
     2821 agattaacca gcaaatggtt cctcaaatcg tattgatatt tacccggcgt attgagcacc
     2881 accatctgac actccgttcc cgcatcaaac gactggaccg aaaaacgatt tacttttcac
     2941 gtgctgtgga aattcacgaa aaaattattg ggtcattcat cgaaaaacat atgttctgat
     3001 gactggagtc atcctccaga aatatacgtc aactgaacaa tatcgatttt ttatgggatt
     3061 ctatacactt ttccatatcc ctctgaaaat cagtcatcag ctcaccaaca cctcttctga
     3121 aacttgtatt aaacttattg cttaaataga tgatataaca tctcttccat tgcactgaat
     3181 cggcgaaaac tgccgtttca ataatctcaa caatattatt tatcggctaa ctttatcagt
     3241 aaacaatgga atgtcacact gatgaaatga acattcagag taagatgata aaattcatat
     3301 aaacgccatt gctgaatatt atttatttca tacagataaa cgataaaata caaaagggta
     3361 tatttctcaa tgtaacagtc aattattcat ctccactggc gagcacggtc agcacacaaa
     3421 caaagttagt cctcctgctt aatcattgcg tatcagaaac taaaaaagaa aaaattctgc
     3481 agacaactta acaatcttca tcacatttcc tgaagacaga catatccaca aacggtagtc
     3541 agcgttgaat taacaagtct tacagagcta tacgtagctt ataaagccac tcaatgttca
     3601 tcatcacccg actacttatc tggaatacca ccagtataaa ctcatcaata aaaacacctg
     3661 ttgttaaaat aagaatactg tcagcagtaa attattaaaa caacatctgt ccacactatc
     3721 tgttctttta acgccaacgg cagcgaaaat agtgcatcac agccttcttt tttctttcgc
     3781 caccactcag acaaacacaa cactcacacc cacaccacaa aacagaatca aaacataaaa
     3841 ttaacaataa cagtcaaaag gaaaacacag aatattgagg tggactgtca ccaggacagc
     3901 gcatacgacg catgtgatca gcacatcccg ttcttcgctg gcatccccaa aatgcagaac
     3961 aaacaatcat aacattatga atataaaaga aaatatgatt tttcaccatg aaaaaaacat
     4021 caatgtatct ctgtataaaa ataatgataa catgacataa acatttaaaa acataaataa
     4081 acagcaacaa aaacatttga taatcactta gtgattaaat acaattaaga cgattttgtt
     4141 ataagagagg caccatggag tgagataatg aataacgaac agatgcttat caaatgcaga
     4201 gatccccgta aatgtcggtt ttatgccgaa tatctgaatc acaaataccc taatgaattc
     4261 cctcactttg cgcctgacaa cccacaaaat tgtatcagga atgttaattt atttttaaag
     4321 gaaaagtatt gtaatgtttc acctcccccc tttggtactt ttggtactac tgaagaagaa
     4381 gcactcaata acgaaattaa tgagcttgaa attattgccg ggcaggcaat actgcctgaa
     4441 gagagttttc tctggttgaa aactgacgaa cttgcgactt tctttacctg gactactatt
     4501 tatctgtcga aggaagacca tacaaaattc ccgaaaataa accataattc atcaggaata
     4561 aaaaacggaa ttgaagtcag gaaaatacga acagttaccg atgaaacgtt aaacacttac
     4621 aaaaaaagta accttccgga taatccatca tcccataacg aacgaataaa agtaatcatt
     4681 tcatattttg actcctgcca ttctttaatt aataaaaatg caaaaagcat atacctcgag
     4741 gaactcaggc aggcgtggct taaaaagaaa agcgcttgtc aaaagtttaa gtggctcctt
     4801 cctgacgatg atgaaatgtg tcagtggttc tggacaagac tgaaacaaaa acaggatgaa
     4861 aaacatccag tacataaccc agtgacccgg tggttcatac catcttcaac gaaagaatat
     4921 tatcttgcct ctcttgtcgc ctttgcttta tggaaagaag cgcctgatac cattgaactg
     4981 ttcagaacac gaataaatca tgcctggcat cagaaaaaac aaaaggacaa atatcggagt
     5041 gatggtaaaa aggccattaa tatacatata agaagcaatg tgaaagaaat gcttgatgag
     5101 ctcagaactg tttacggtct gagaacagga gagttttttg aaatgctgat tacagagaaa
     5161 tatcttgaac acaagcgaag gaagtaattc attacctgtt acattctaaa taatctcaga
     5221 cagatattat ctccgtgaat cccccgccac ctttccggtg cgcggggttt tgtctttttt
     5281 caccgggaat acatgtatga atccgtctga tgccattgag gcaattgaaa aaccgctctc
     5341 ctccctgcct tactcacttt cccgtcacat cctggaacat ctgcgcaaac tcaccagtca
     5401 cgaacccgtg attggcatta tgggtaaaag cggggccggt aaatcttcac tctgtaatgc
     5461 actgtttcag ggggaggtca caccggtcag tgatgttcac gccggcaccc gggaagtgca
     5521 gcgcttccgt ctgagtggtc acggtcacag catggttatc actgacctgc ccggggtggg
     5581 cgagagccgg gacagggatg cagagtatga agccctgtac cgtgacattc tgcctgaact
     5641 ggacctggtg ctgtggctga ttaaagccga tgaccgggcc ctgtctgtgg atgagtattt
     5701 ctggcgacac atcctgcagt gcggacatca gcaggtgctg tttgtggtga cgcaggctga
     5761 caaaacggaa ccctgccatg aatgggatat ggcaggtatt cagccttctc ccgcacagga
     5821 acagaacatt cgtgagaaaa cggaggcggt attccgtctg ttccggcccg tacatccggt
     5881 tgttgccgta tcggcccgca ccggctggga actggatacg ctggtcagtg cgctcatgac
     5941 agcgcttccc gaccatgccg ccagccccct gatgacccga ctgcaggacg agctgcgcac
     6001 ggagtctgtc cgctctcagg cccgtgaaca gtttaccggt gcggtggacc ggatatttga
     6061 cacagcggag agcgtctgtg ttgcctctgt tgtccgtacg gccctgcgcg ctgttcgtga
     6121 caccgtggtc tctgttgccc gcgcggtatg gaactggatc ttcttctgaa cctgttgtgg
     6181 atgatgtcct ccctgcctct gagtctgctc acaaaagcgc tgttttcgtt actgtctctc
     6241 ttgtccgtgc aatagactga taatagaata aaacgatcga tacctatttt accgatcgtt
     6301 aatatcgatc gttatgctaa taataactcc tgttagcaac gtgcgcagat acacacagac
     6361 atgagattca gggaacaaca gagccacacg tcagaaactt ccgtcagctg gacctccggc
     6421 actgtaaccc tttacctgcc ggtatccaca tctgtggata ccggcttttt tattcaccct
     6481 cactctgatt aaggaaatgc tgatgaaacg acatctgaat acctgctaca ggctggtatg
     6541 gaatcacatt acgggcgctt tcgtggttgc ctccgaactg gcccgcgcac agggtaaacg
     6601 tggcggtgtg gcggttgcac tgtctcttgc cgcggtcacg tcactcccgg tgctggctgc
     6661 tgacatcgtt gtgcacccgg gtgaaacagt gaatggcgga acactggtaa accatgacaa
     6721 ccagtttgta tccggaacag ctgatggcgt gactgtcagt accgggcttg agctggggcc
     6781 ggacagtgac gaaaacaccg gcgggcaatg gataaaagcg ggtggcacag gcagaaacac
     6841 cactgtcacc gcaaatggtc gtcagattgt gcaggcagga ggaactgcca gtgatacggt
     6901 tattcgtgat ggcggagggc agagccttaa cggactggcg gtgaacacca cgctggataa
     6961 cagaggtgag cagtgggtac acgggggagg gaaagctgcc ggtacaatta ttaaccagga
     7021 tggttaccag accataaaac atggcggact ggcaaccgga accatcgtca acaccggtgc
     7081 agaaggtggt ccggagtctg aaaatgtgtc cagcggtcag atggtcggag ggacggctga
     7141 atccaccacc atcaataaaa atggccggca ggttatctgg tcttcgggga tggcacggga
     7201 caccctcatt tacgccggtg gtgaccagac ggtacacgga gaggcacata acacccgact
     7261 ggaggggggt aaccagtatg tacacaacgg tggcacggca acagagacgc tgataaaccg
     7321 tgatggctgg caggtgatta aggaaggagg aactgccgcg cataccacca tcaaccagaa
     7381 aggaaagctg caggtgaatg ccggcggtaa agcgtctgat gtcacccaga acacgggcgg
     7441 tgcactggtt accagtacag ctgcaaccgt caccggcaca aaccgcctgg gagcattctc
     7501 tgttgtggag ggtaaagctg ataatgtcgt actggaaaat ggcggacgcc tggatgtgct
     7561 gaccggacac acagccacta atacccgcgt ggatgatggc ggaacgctgg atgtccgcaa
     7621 cggtggcacc gccaccaccg tatccatggg aaatggcggt gtactgctgg ccgattccgg
     7681 tgccgctgtc agtggtaccc ggagcgacgg aaaggcattc agtatcggag gcggtcaggc
     7741 ggatgccctg atgctggaaa aaggcagttc attcacgctg aacgccggtg atacggccac
     7801 ggataccacg gtaaatggcg gactgttcac cgccaggggc ggcacactgg cgggcaccac
     7861 cacgctgaat aacggcgcca tacttaccct ttccgggaag acggtgaaca acgataccct
     7921 gaccatccgt gaaggcgatg cactcctgca gggaggcgct ctcaccggta acggcagcgt
     7981 ggaaaaatca ggaagtggca cactcactgt cagcaacacc acactcaccc agaaagccgt
     8041 caacctgaat gaaggcacgc tgacgctgaa cgacagtacc gtcaccacgg atgtcattgc
     8101 tcagcgcggt acagccctga agctgaccgg cagcactgtg ctgaacggtg ccattgaccc
     8161 cacgaatgtc actctcgcct ctggtgccac ctggaatatt cccgataacg ccacggtgca
     8221 gtcggtggtg gatgacctca gccatgccgg acagattcat ttcacctcca cccgcacagg
     8281 gaagttcgta ccggcaaccc tgaaagtgaa aaacctgaac ggacagaatg gcaccatcag
     8341 cctgcgtgta cgcccggata tggcacagaa caatgctgac agactggtca ttgacggtgg
     8401 cagggcaacc ggaaaaacca tcctgaacct ggtgaacgcc ggcaacagtg cgtcggggct
     8461 ggcgaccagc ggtaagggta ttcaggtggt ggaagccatt aacggtgcca ccacggagga
     8521 aggggccttt atccagggga ataagctgca ggccggtgcc tttaactact ccctcaaccg
     8581 ggacagtgat gagagctggt atctgcgcag tgaaaatgct tatcgtgcag aagtccccct
     8641 gtatgcctcc atgctgacac aggcaatgga ctatgaccgg attctggcag gctcccgcag
     8701 ccatcagacc ggtgtaagcg gtgaaaataa cagcgtccgt ctcagcattc agggcggtca
     8761 tctcggtcac gataacaacg gcggtattgc ccgtggagcc acgccggaaa gcagcggcag
     8821 ctatggcttc gtccgtctgg agggtgacct gctcagaaca gaggttgccg gtatgtctgt
     8881 gaccgcgggg gtatatggtg ctgctggcca ttcttccgtt gatgttaagg atgatgacgg
     8941 ctcccgtgcc ggcacggtcc gggatgatgc cggcagcctg ggcggatacc tgaatctgat
     9001 acacaacgcc tccggcctgt gggctgacat tgtggcccag ggaacccgcc acagcatgaa
     9061 agcctcatcg gacaataacg acttccgcgt ccggggctgg ggctggctgg gttcgctgga
     9121 aaccggtctg cccttcagta tcactgacaa tctgatgctg gagccgcaac tgcagtacac
     9181 ctggcaggga ctctccctgg atgacggcca ggataacgcc agttatgtga agttcgggca
     9241 tggcagtgca caacatgtgc gtgccggctt ccgtctgggc agccaccacg atatgaactt
     9301 tggtaaaggc acctcatccc gtgacaccct gcgcggcagt gccaaacaca gtgtgcgtga
     9361 actgccggtg aactggtggg tacagccttc tgttatccgc accttcagct cccgggggga
     9421 catgagcatg ggtacagccg cagccggcag taacatgacg ttctcaccgt cacagaatgg
     9481 tacgtcactg gacctgcagg ccggactgga agcccgtgtc cgggaaaata tcaccctggg
     9541 cgttcaggcc agttatgccc acagcatcaa cggcagcagc gctgaaggtt ataacagtca
     9601 ggccacactg aatgtaacct tctgataatt ctgcattgcc tctctgtggt cccggtcatg
     9661 atgaccggga cccggacggg ggaaaacgtt tcagtgccac attcactggc attcacaata
     9721 acatgatatt catcacggag tgactatgtt acagatagtc ggcgcgctga ttctgctgat
     9781 cgcaggattt gccattcttc gccttttgtt cagagcatta accagcacag cgtctgcgct
     9841 ggcagggttc atattgctgt gtctgttcgg tccggcttta ctggctggct atatcactga
     9901 acgcataacc cggttattcc atattcgctg gctggcaggc gtatttctga cgattgccgg
     9961 aatgatcatc agcttcatgt ggggacttga tggtaaacat atcgcactgg aggctcatac
    10021 ttttgactct gtaaaattta ttctgaccac cgctctcgcc gctggtctgc tggctcttcc
    10081 cgtgcagata agaaccattc agcagaacgg gctcacacct gaagatatca gcaaggaaat
    10141 taacgggtat tactgctgtt tttatactgc ttttttcctt atggcgtgtt ctgcatacgc
    10201 accattgatc gcattgcagt tcgatatttc accctcactg atgtggtggg gcgggttgtt
    10261 gtactggctg gctgcattag tgacgctgct atgggcggcc agccagatcc aggcgctgaa
    10321 aagactgacc agtgccatca gccagacact ggaagaacaa ccggtgctca acagtaaatc
    10381 gtggctgagc agtttgcaaa acgattacag ccttcctgaa acgctgacgg agcgcatctg
    10441 gctgacactc atttcacaac ggatttcccg gggagaactg agggaatttg aactggcaga
    10501 cggaaactgg ttactgaaca atgcctggta tgaaagaaac atggcagggt ttaacgaaca
    10561 gttgaaagag aacctgtcat tcacacctga tgaactgaaa acgctcttcc ggaaccgcct
    10621 gaatttatca ccggaagcga atgacgattt tctcgatcgt tgcctggacg gcggtgactg
    10681 gtaccccttt tcagaaggcc gccgttttgt atcattccac cacgtggatg agcttcgtat
    10741 ctgtgcctcc tgcgggctga cagaagtaca tcatgccccg gaaaatcata agccggctcc
    10801 ggaatggtac tgctcctctc tttgtcacga aacagaaaca ctgtgtcagg acatttatga
    10861 acgttcttac accggtttta tttccgatgc aacggcgaat ggtctgattc tcatgaaact
    10921 gccggaaacc tggagtacaa atgagaaaat gtttgcttcc ggagggcagg gacatgggtt
    10981 tgccgctgaa cggggaaacc atattgtcga cagagtccgt ctgaaaaacg cacggatcct
    11041 cggtgataat aatgccagaa atggagcaga cagactggtc agcggaacag aaatccagac
    11101 gaaatattgt tcaactgcag cccgtagcgt cggtgcggca ttcgacggac aaaacggaca
    11161 gtatcgttac atgggaaatc atggtcccat gcaactggaa gtcccccgtg atcagtatgc
    11221 cggcgctgtg gaaaccatga agaataagat ccgcgaaggt aaagtacccg gtgtaaccga
    11281 tcccgcagaa gcgtcccggc tgattcgtcg gggacatctg acttataccc aggcccgtaa
    11341 tatcacccgg ttcgggacca tcgaatcggt cacttatgat attgccgagg ggtcggttgt
    11401 cagtctggcg gccggaggga tcagttttgc cctgacggca tcggtcttct ggctcagcac
    11461 cggcgatcgc gatgctgccc tgcagacagc tgctgtccag gcaggaaaaa ccttcacccg
    11521 cacactggct gtctacgtca caacccagca acttcaccgg ctcactgttg ttcagggtat
    11581 gctgaagcat attgattttt cgacggccag cccgactgtc cggcaggcgc ttcagaaggg
    11641 gaccggtgca ggaaatatca gtgccctgaa caaagtgatg aagggtacgc tggtgacatc
    11701 tctggcactg gtagctgtca caaccagccc tgacatgatc aaaatgttgc ggggacggat
    11761 ctccggtgcg cagttcatca ggaatcttgc cgtggcatct tccggtgtgg caggtggtgc
    11821 tgtcgggtca gtggcgggcg ggatattgtt cagtccactg ggaccatttg gtgcactgac
    11881 agggcgtgtg gttggcggtg ttctgggggg aatgattgcc tccgctgtat caggaaaaat
    11941 tgccggagcg ctggttgaag aagatcgcgt caaaattctg gcaatgattc aggagcaggt
    12001 gacatggctt gccggcagtt tcctgctgac cggacatgag attgaaaatc tgaacgcgaa
    12061 tctggcccgt gttatcgatc agaatgctct ggagatcatt ttcgccgccg gtatacaaca
    12121 acgggccgcg accaatatgt taatcaaacc actggtggtc agtatcatca ggcaacgccc
    12181 cgtcatggaa tatgatgcat cccatctcgg caagatggtt aaccggctgg aagaagcatt
    12241 ccccccggag ctcccggcat aacgtgtgtt ctgctccttt taggttttat cacctgattt
    12301 gccagtgtca tcgtcatgac tcagagaggt aaataccatg attcacctgt tcaaaacctg
    12361 catgattacc gccttcattc tggggttaac gtggtctgcc ccactccggg cacaggatca
    12421 acgttacatc agtatacgca atacagatac gatatggctc ccgggaaata tttgtgctta
    12481 ccagttccgg ctggataatg gcggaaacgg tgaaggattt ggccccctca ccatcactct
    12541 gcaactcaaa gacaaatatg gtcagacgct ggtgaccaga aaaatggaaa cggaagcttt
    12601 tggtgacagt aatgccacgc gaaccacaga cgcatttctg gaaacggagt gcgtggaaaa
    12661 tgtcgccaca accgaaatca ttaaagcaac tgaagaaagt aacggccatc gtgtcagtct
    12721 gccgttatcg gttttcgatc cccaggacta ccatccactg ctgattaccg tttccggaaa
    12781 aaatgttaac tgatccctga agtcagtttc atgcttcagc ctctgccaca acgtatggca
    12841 gaggctattt atcaggagaa accagatatg ccgggttaca cacagagtat gtcctggctg
    12901 aaggatcttt ctcatacggg caggcggttg ctgtcataac cgcttaccgc aatgtgttta
    12961 tccaggatga cccgggaatg catttccgtc gggttatccg caacgcggaa ggtcagcgac
    13021 gctgacgcag ccggaactct gaagctgatg ccggaaaaca gcttaatgcg tggctcgcca
    13081 gtggagggct tctcagacaa taaacttcat tgatttcatc catcaggccg cgtcttctcc
    13141 gggagacgcg gcttttttca tttgttgcac cagtaaatct taaccaccga taaggagcaa
    13201 agtatgcaat tagtcagtcg ttttggttat gcgaaccaga tacgccgtga ccgtccgctg
    13261 acacatgaag agctgatgca ccatgtgccg gggattttcg gggaagagaa acacacgtcc
    13321 cgaagccaga actatacgta catccccacc atcaccgtac tggaaagtct gcagcgggaa
    13381 ggcttccagc cctttttcgc ctgccagacc cgtgtgcgcg acccgggccg ccggggatac
    13441 acaaaacaca tgctgcgtct gcggcgggcc ggagagataa acggagaaca tgtccctgaa
    13501 attattctgc tcaactccca tgacggtacc tccagctacc agatgctgcc gggttacttc
    13561 agattcgtct gccagaacgg gtgtgtctgt ggtcagtctc tgggggaagt gcgtgttccg
    13621 caccggggaa acgtggtgga gaaagttatt gaaggggctt acgaagtggt gggcgtgttt
    13681 gaccggattg aggagaaacg tgatgccatg cagtcgctgg tcctgccgcc accggcacgc
    13741 caggcgctgg cacaggcggc actgacttac cgttatggtg acgaacatca gcccgtcacc
    13801 accgccgaca ttctgacgcc acgacgccgg gaggattacg gtaaggacct gtggagtact
    13861 tatcagacca tccaggagaa tatgctgaaa ggcgggattt ccggccgcag tgcaaaagga
    13921 aaacgtatcc acacccgtgc cattcacaac atcgacaccg atattaagct caaccgcgca
    13981 ttgtgggtga tggcagaaac gctgctggag agcctgcgct ga
//
LOCUS       DAJTSI010000103        13964 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000103 DAJTSI010000000
VERSION     DAJTSI010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13964
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.103"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="OO327_002804"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="OO327_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6019272.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLVLLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="OO327_002805"
     CDS             2219..2767
                     /locus_tag="OO327_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR6019273.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="OO327_002806"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="OO327_002806"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR6019274.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="OO327_002807"
     CDS             complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="OO327_002807"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR6019275.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSSDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="OO327_002808"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="OO327_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR6019276.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKTVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="OO327_002809"
     CDS             complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="OO327_002809"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR6019277.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="OO327_002810"
     CDS             complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="OO327_002810"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR6019278.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9765..12380
                     /gene="pepN"
                     /locus_tag="OO327_002811"
     CDS             9765..12380
                     /gene="pepN"
                     /locus_tag="OO327_002811"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR6019279.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="OO327_002812"
     CDS             complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="OO327_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6019280.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13351..>13964)
                     /gene="ssuC"
                     /locus_tag="OO327_002813"
     CDS             complement(13351..>13964)
                     /gene="ssuC"
                     /locus_tag="OO327_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCR6019281.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         3550 a         3563 c         3527 g         3324 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggtctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg ggaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga gctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgctgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcaa ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agttttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggagcttt gtgaacaccg
     6361 tcatatttcc ataggaacgt gatgatattt acagcaattt ttatctattt atatgattta
     6421 cttatattta aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc
     6481 acaaaatcct ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag
     6541 ctaagaaaaa actaatccgc aataagagta cgggttattt tttgtgcgta aatgttgcaa
     6601 tgtaatattc cgtaacagga tgatcgttat ggcgggggtg caaatagatt gcttgactta
     6661 acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg
     6721 aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt
     6781 tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg
     6841 gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca
     6901 tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga
     6961 tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga
     7021 ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa
     7081 agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg
     7141 ggttttcaaa cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct
     7201 gcgcgaaatc ggcttcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt
     7261 cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca
     7321 gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca
     7381 gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc
     7441 cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata
     7501 cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt
     7561 tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg
     7621 tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc
     7681 gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc
     7741 gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg
     7801 ggtaagtgtc tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa
     7861 attgttgccc ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg
     7921 tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta
     7981 caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc
     8041 ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga
     8101 gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata
     8161 aacacttgtc cacccgtaaa tgggggggat acctatgtta cctggcatct gcaatcagac
     8221 aagcagaatt cgcaaatgca gcgaaagatt tcggaattaa aggtaaaaaa gggagccgat
     8281 tagctccctg atgatattaa ctggcttttt taatatgcgg aaggtcgaac gctttgcgca
     8341 gcgcccgaac aaacgcttta tcatggcaga tagttttgcc agggctgtca gaaagtttcg
     8401 ccaccggttt accgttacac tctaccaact taatcacaat attcaggggt tttacctggg
     8461 ggatatcgca ggtcaggcga gtcccaatac caaaacttaa ttgcacgcgg gaagagaagt
     8521 ggcggtatag ctcaaccgct ttgcgtaaat ccagattgtc agagaaaacc agcgttttac
     8581 tctgtggatc aattcccagc ttttcataat gtgcaatggc tttttcaccc cattcaaccg
     8641 ggtcgccaga gtcatgacgc aggccctgat accgactagc gaactcgaca ccgaaatcac
     8701 gcaggaaagc atccatagtg atgcagtcgg ttaatgcaat gccaagttgg tcgggatact
     8761 cttccagcca ggcagcaagt gcagctcgct ggctgttggc tagatccggg ctgatttgct
     8821 gatgtgcctg gaaccattcg tgtgcctgtg ttcccatcgg cgtgagggaa agccgacgcg
     8881 ccagatcgta gttgctggtg cccacgaacc aggattcctg ttgcagacgc ttaacgatgg
     8941 tttcttgtac ttcgcgagaa aaacggcgac gggtgccaaa atccatcaga tggaagcgcg
     9001 acatatcaag accggcggtt aacgccgaga agtcgactaa tttgctttcc agcgtgtcga
     9061 gggcttgcgc aacgtcggcc tgcggtgagc gatagcgatg taccatttca ctgataaccg
     9121 ccagcaaagg aacttcccag aggatgactt cacgccacgg accgcttaag cgaatatcca
     9181 gcttgccatt atcgttggac acggtgactt gttccgggtt aaagcggaac tcgcgtaacc
     9241 agttaagata gtcggcctta aagaaaggca gggcagagag ccactgatat tcatcatcct
     9301 gcaggcgcag gtgctgcatc gcctgaatct gttcacgaat agcatcggca taaataccca
     9361 gcagatcgtc acctcggcaa cgaaactccg ccgcgacatg cacatcgtaa tagtgatgaa
     9421 acacggcttg ctgcatatgc aacttataag catctgtatc cagcaacgag tgcagaacag
     9481 gagaagcgaa ttgtgtcata ggtgcgcagt agcgtcctcg tacaggagcg tttagtacaa
     9541 taaacatctt caggaactgc tggagtatac cttgtttcgc aatttattga accccgatca
     9601 caccatatgc cacctttctg gtcgatggca ttacgcggtg tatgttataa aaatgtagca
     9661 ataaaggcgt ttgtacctga aaagatgaag attctgcata gcgcgattta cgcaacagga
     9721 atagactgaa caccagactc tataaaagat gctaaaggtt atttatgact caacagccac
     9781 aagccaaata ccgtcacgat tatcgtgcgc cggattacca gattactgat attgacttga
     9841 cctttgacct cgacgcgcaa aagacggtcg ttaccgcggt cagccaggct gtccgtcatg
     9901 gtgcatcaga tgctccgctt cgtctcaacg gcgaagacct caaactggtt tctgttcata
     9961 ttaatgatga gccgtggacc gcctggaaag aagaagaaga gggcgcactg gtcatcagta
    10021 atttgccgga gcgttttacg cttaagatca ttaatgaaat aagcctggcg gcgaatacgg
    10081 cgctggaagg gctttatcag tcaggcgatg cgctttgcac ccagtgtgaa gccgaaggtt
    10141 tccgccatat tacgtattat ctcgaccgcc cggacgtgct ggcgcgtttt accaccaaaa
    10201 ttattgccga taaaatcaaa tatcccttcc tgctttccaa tggtaaccgc gttgcgcaag
    10261 gcgaactcga aaacggacgc cattgggtac agtggcagga cccgttcccg aaaccgtgct
    10321 acctgtttgc gctggtggca ggcgactttg atgtactgcg cgataccttt accacgcgtt
    10381 ctggtcgcga agtagcactg gagctgtacg tcgatcgcgg caaccttgat cgcgcgccgt
    10441 gggcgatgac ctcgctgaaa aactccatga aatgggatga agaacgcttt ggcctggagt
    10501 atgacctcga catctatatg atcgtcgcgg tggatttctt caatatgggc gcaatggaga
    10561 ataagggtct gaatatcttt aactccaaat atgtgctggc ccgcaccgac accgccaccg
    10621 acaaagatta cctcgatatt gaacgcgtta tcggccatga atatttccat aactggaccg
    10681 gtaaccgagt gacctgccgc gactggttcc agctcagcct gaaagaaggt ttaaccgtct
    10741 tccgcgatca ggaattcagc tctgaccttg gttcccgcgc agttaaccgc atcaataatg
    10801 tgcgcaccat gcgcggattg cagtttgcgg aagacgccag cccgatggcg cacccaatcc
    10861 gcccggacat ggtcattgag atgaacaact tctacaccct gaccgtttac gagaagggcg
    10921 cggaagtgat ccgtatgatc cacaccctgc tgggcgaaga aaacttccag aaagggatgc
    10981 aactctattt cgagcgtcat gatggtagcg cagcgacctg tgacgacttt gtgcaggcga
    11041 tggaagatgc gtcgaatgtt gatctctccc atttccgccg ttggtacagc cagtccggta
    11101 cgccggttgt gaccgtcaaa gacgactaca atccggaaac tgagcagtac accctgacca
    11161 tcagccagcg cacgccagcc acgccggatc aggcagaaaa acagccgctg catattccgt
    11221 ttgccatcga attgtatgac aacgaaggta aagtgatccc gttgcagaaa ggcggtcatc
    11281 cggtgaattc cgtgctgaac gtcacccagg cggaacagac ctttgttttt gataatgtct
    11341 acttccagcc ggtgcctgcg ctgctgtgcg aattctctgc gccagtgaaa ctggaatata
    11401 agtggagcga tcagcaactg accttcctga tgcgtcatgc gcgtaatggt ttctcccgtt
    11461 gggatgcggc gcaaagtttg ctggcaacct acatcaagct gaacgtcgcc cgtcatcagc
    11521 aagggcagcc gctgtctctg ccggtacatg tggctgacgc tttccgcgcg gtgctgctcg
    11581 atgagaagat tgatccggcg ctggcggcag aaatcctgac gctgccttct gtcaatgaaa
    11641 tggctgaact gttcgatatc atagacccga ttgctattgc cgaagtacgc gaagcactca
    11701 ctcgtactct ggcgactgaa ctggcggatg agctgctggc tatttacaac gcgaattacc
    11761 agagcgagta ccgtgttgag catgaagata ttgcaaaacg cactctgcgt aatgcctgcc
    11821 tgcgcttcct tgcttttggt gaaacgcatc tggctgacgt gctggtgagc aagcaatacc
    11881 atgaagcaaa caatatgacc gatgcgctgg cagcgctttc tgcggcggtt gctgcacagc
    11941 tgccttgccg tgacgcgctg atgcaggagt acgacgataa gtggcatcag gacggtctgg
    12001 tgatggataa atggtttatc ctgcaagcca ccagcccggc ggcgaatgtg ctggagacgg
    12061 tgcgtggtct gttgcagcat cgctcattta ccatgagcaa cccgaaccgt attcgttcgt
    12121 tgattggtgc gtttgcgggc agcaacccgg cagcgttcca tgccgaagat ggcagcggtt
    12181 accagttcct ggtggaaatg cttaccgacc tcaacagccg taacccgcag gtagcttcac
    12241 gtctgattga accgctgatt cgcctgaaac gttatgatgc caaacgtcag gagaaaatgc
    12301 gcgcggcgct ggaacagttg aaagggctgg aaaatctctc tggcgatctt tacgagaaga
    12361 taactaaagc actggcttga tacttttgag ccaaaatgcc tgatgcgacg cttgacgcgt
    12421 cttatcaggc ctacaactcc gttgaaattg ctggttttgt aggctggata aggcgttcac
    12481 gccgcatccg gcagccgtgc ccaactgcct gatgcgatgc ttgacgtgtc ttatcaggcc
    12541 tacaagtccg ttgaaaatgc tggttttgtg cgccacatcc ggcatattaa ccatgacgcc
    12601 gaatcagttg ttcagaatgc ccacgccgca ttacccgttg caatacttcc gcttccagct
    12661 ccgctaatcg caccgaccct aatcgacgcg gacgggggat atccactgtc aaatccagac
    12721 taatttttcc ctcttcaatt aacaacaccc ggtcagccat tgctaccgct tcgctcacat
    12781 catgcgtcac caacagtacg gtaaagccgt gctgctgcca aagtgacaca atcaaatcct
    12841 gcatctcgag tcgcgttaag gcgtccagtg ccccgagcgg ttcatcaagc aacaataaac
    12901 ccggtcgatg aatcaacgct cttgccagcg ccacacgctg tttctgcccg ccagaaagtg
    12961 cggcaggcca ttccccggcg cgattctcca gtcctgccgc agccaacgcc cgacgcgcgg
    13021 catcgcgcca ctgaccttta aggcctaacc caacgttatc aatcaccgat ttccacggca
    13081 gcagacgcgc atcctgaaac atcattcgcg tatcatcctg aatttcagcc agcggtgtgg
    13141 tgcccgctaa cacatcgcct gcggttggcg tttccagacc ggccagcagg tgcagcaggg
    13201 tacttttacc accaccgctg cggcccacca ccgccacaaa ctgacctgcc ggaatatgta
    13261 aatccagttg gttcaggacg atattttccg cgtaatgttt gcttactgca ttgagcaaca
    13321 atggtgtgcc ctggttcaga cgagcagtat tcatatcgtg gcctccttca aatgataagc
    13381 cgggttccag cgcaaccaga ggcgctctaa cagctgcgcg ctgacgtcag caagtttgcc
    13441 gagcagggcg taaagaataa tagcgactac caccacgtct gtttgcaaaa actctcgcgc
    13501 attcatcgcc agataaccaa tgccggaatt ggcagaaatg gtttcggcaa caatcagcgt
    13561 cagccacatc aggcccaacg caaaacgcac gccgaccata attgagggca gggcatcggg
    13621 caggatcaca tggataaaca gcggtatgcc ggataatcca tagctacgcg ccatctccac
    13681 cagcccgcga tcgatattac ggatgccatg ccaggtgttg atataaatgg ggaacagcgt
    13741 accgagcgcc accagaaaga ttttcgcgga ttcatcaatg ccaaaccaca aaatcaccag
    13801 cgggatcagc gccagatgcg gcacgttgcg caacatctga attgaggtat ccagcagcct
    13861 ttctccccag cgcgacagtc cgctaatcag tcccagaatc aggcccagcg atccgccaat
    13921 tgaaaagcca atcagcgccc gccaggaact gatcgccaga tgct
//
LOCUS       DAJTSI010000104        13881 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000104 DAJTSI010000000
VERSION     DAJTSI010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13881
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.104"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(88..624)
                     /gene="ssb1"
                     /locus_tag="OO327_002814"
     CDS             complement(88..624)
                     /gene="ssb1"
                     /locus_tag="OO327_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR6019282.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            878..3700
                     /gene="uvrA"
                     /locus_tag="OO327_002815"
     CDS             878..3700
                     /gene="uvrA"
                     /locus_tag="OO327_002815"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR6019283.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3735..4091)
                     /locus_tag="OO327_002816"
     CDS             complement(3735..4091)
                     /locus_tag="OO327_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6019284.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4095..4511)
                     /locus_tag="OO327_002817"
     CDS             complement(4095..4511)
                     /locus_tag="OO327_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR6019285.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4622..5335)
                     /gene="aphA"
                     /locus_tag="OO327_002818"
     CDS             complement(4622..5335)
                     /gene="aphA"
                     /locus_tag="OO327_002818"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR6019286.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5737..5941
                     /locus_tag="OO327_002819"
                     /pseudo
     CDS             5737..5941
                     /locus_tag="OO327_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6444..7637)
                     /gene="tyrB"
                     /locus_tag="OO327_002820"
     CDS             complement(6444..7637)
                     /gene="tyrB"
                     /locus_tag="OO327_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR6019287.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7890..8969)
                     /gene="alr"
                     /locus_tag="OO327_002821"
     CDS             complement(7890..8969)
                     /gene="alr"
                     /locus_tag="OO327_002821"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR6019288.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9022..10437)
                     /gene="dnaB"
                     /locus_tag="OO327_002822"
     CDS             complement(9022..10437)
                     /gene="dnaB"
                     /locus_tag="OO327_002822"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6019289.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10520..11503
                     /locus_tag="OO327_002823"
     CDS             10520..11503
                     /locus_tag="OO327_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR6019290.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11669..11911)
                     /gene="pspG"
                     /locus_tag="OO327_002824"
     CDS             complement(11669..11911)
                     /gene="pspG"
                     /locus_tag="OO327_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR6019291.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12045..13082)
                     /gene="dusA"
                     /locus_tag="OO327_002825"
     CDS             complement(12045..13082)
                     /gene="dusA"
                     /locus_tag="OO327_002825"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR6019292.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13445..>13881)
                     /locus_tag="OO327_002826"
     CDS             complement(13445..>13881)
                     /locus_tag="OO327_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HCR6019293.1"
                     /translation="LTFVNACQEHDDNSLKLFASLLISLSYSSAYADLSETVYINENN
                     ESYLKAQFEKLYQRDMKKYLGEMKRLADGGEMNFDGYLDKMSHLVNEGTLDPDILSKM
                     RDAAPQLISFAKSFDPTSKEEIKILTDTSKLIYDLFGVKSEK"
BASE COUNT         3528 a         3558 c         3387 g         3408 t
ORIGIN      
        1 ctccataacg gaggcttctt ttttatggct ggcagatgct ttaatcatcc accttaaaac
       61 aatataacct attgttttaa tgacaaatca gaacggaatg tcatcatcaa agtccatcgg
      121 cggctcgtta gacggcgctg ccggagcgga ctgctgcggg cgagactgcg cgccgccgct
      181 gaactgattg ccaccctgcg gctgctgagg ctgaccccaa ccgccctgcg gctgaccacc
      241 accgatattg ccacctgccg gagcgccacc accctgacga ccacccagca tctgcatggt
      301 gccgccaacg ttcaccacga cttctgtggt gtagcgatcc tgaccggatt gatcggtcca
      361 tttacgggta cgcagctgac cttcgatata aacctgagaa cctttacgca gatattcgct
      421 cgccacttct gccagtttgc cgaacagcac aacgcggtgc cattcagtct gttctttcat
      481 ctcgccggtc gctttatcac gccaggattc ggaagtagcc agcgtaatgt tggcaactgc
      541 gccaccattt ggcatgtagc gtacttccgg gtcctgaccc agattaccaa cgagaataac
      601 cttgtttacg cctctgctgg ccatgttcgt gtctcctgaa aaaaatcgtt ctgaataagt
      661 gtaaacgcgc gattgtacca ttaccaatag cgcttttact atgttgtgac ctcggttccg
      721 ggaaacaaac ctggccagac attgttacac aacactccgg ataatgcatt ccaatactgt
      781 atattcattc aggtcaattt gtgtcataat taaccgtttg tgatcgccgg tagcaccatg
      841 ccaccgggca aaaaagcgtt taatccggga aaggtgaatg gataagatcg aagttcgggg
      901 cgcccgcacc cataatctca aaaacatcaa cctcgttatc ccccgcgaca agctcattgt
      961 cgtgaccggg ctttcgggtt ctggcaaatc ctcgctcgct ttcgacacct tatatgccga
     1021 agggcagcgc cgttacgttg aatccctttc cgcctacgcg cggcagtttc tgtcactgat
     1081 ggaaaagccg gacgtcgatc atattgaggg gctttctcct gccatctcaa ttgagcagaa
     1141 atcgacgtct cataacccgc gttctacggt ggggacaatc accgaaatcc acgactattt
     1201 gcgtttgttg ttcgcccgcg tcggcgaacc gcgctgtccg gaccacgacg tcccgctggc
     1261 ggcgcaaacc gtcagccaga tggtggataa cgtgctgtcg cagccggaag gcaagcgtct
     1321 gatgctgctc gcgccaatca ttaaagagcg caaaggcgaa cacaccaaaa cgctggagaa
     1381 cctggcaagc cagggttaca tccgtgctcg tattgatggc gaagtctgcg atctttccga
     1441 tcctccgaaa ctggaactgc aaaagaaaca taccattgaa gtggtggttg atcgcttcaa
     1501 ggtgcgtgac gatcttaccc aacgtcttgc ggagtcgttt gaaaccgcgc tggaactttc
     1561 cggtggtaca gcggtagtgg cggatatgga cgacccgaaa gcggaagagc tgctgttctc
     1621 cgccaacttc gcctgcccaa tttgcggcta cagtatgcgt gaactggagc cgcgactgtt
     1681 ttcgtttaac aacccggcag gtgcctgccc gacctgtgac ggccttggcg tacagcaata
     1741 tttcgatcct gaccgcgtga tccaaaaccc cgagctgtca ctggctggcg gtgcgatccg
     1801 tggctgggat cgccgcaact tctattactt ccagatgctg aaatcgctgg cagatcacta
     1861 taagttcgac gtcgaagcgc cgtggggcag cctgagcgcg aacgtgcata aagtggtgtt
     1921 gtacggttct ggcaaagaaa acattgaatt caaatacatg aacgatcgtg gcgatacctc
     1981 catccgtcgt catccgttcg aaggcgtgct gcacaatatg gagcgccgtt ataaagagac
     2041 agaatccagt gcggtacgtg aagaattagc caagtttatc agcaatcgcc catgcgccag
     2101 ctgcgaaggg acgcgtctgc gtcgggaagc gcgccacgtt tatgtcgaga atacgccgct
     2161 gcctgctatc tccgacatga gcatcggtca tgcgatggaa ttcttcaaca atctcaaact
     2221 cgctggtcag cgggcgaaga ttgcggaaaa aattcttaaa gagatcggcg atcgcctgaa
     2281 attcctcgta aacgtcggcc tgaattacct gacactttcc cgctcggcag aaacactttc
     2341 cggcggtgaa gcccagcgta tccgtctggc gagccagatt ggtgcgggcc tggttggcgt
     2401 tatgtacgtg ctggacgagc cgtctatcgg cctgcaccag cgcgataacg agcgcctgtt
     2461 gggtacgctt atccatctgc gcgatctcgg taataccgtg attgtggtgg agcatgacga
     2521 agacgcaatt cgcgccgctg accatgtgat cgatatcggt cctggtgcgg gtgttcacgg
     2581 cggtgaagtg gtcgcagaag gtccgctgga agcgattatg gcggtgcctg aatcgttgac
     2641 cgggcagtac atgagcggta aacgcaagat tgaagtgccg aagaaacgcg ttccggcgaa
     2701 tccagaaaaa gtgctgaagc tgacaggcgc acgcggcaac aacctgaagg acgtgacgct
     2761 cacgctgcca gtcggtctgt ttacctgcat cacaggggtt tcaggttccg gtaaatcgac
     2821 gctgattaac gacacactgt tcccgattgc ccaacgccag ttgaatggtg cgaccatcgc
     2881 cgaaccggca ccgtatcgcg atattcaggg actggagcat ttcgataaag tgatcgatat
     2941 cgaccaaagc ccaattggtc gtactccacg ttctaacccg gcgacctata ccggcgtgtt
     3001 tacgcctgtg cgcgaactgt ttgcgggcgt accggaatcc cgtgcgcgtg gttatacgcc
     3061 aggacgtttc agctttaacg tccgtggcgg gcgctgcgaa gcctgtcagg gcgacggtgt
     3121 gatcaaagtg gagatgcact tcctgccgga tatctacgtg ccgtgcgatc agtgcaaagg
     3181 taaacgctat aaccgtgaaa cgctggaaat taagtacaaa ggcaaaacca tccacgaagt
     3241 gctggatatg accatcgaag aggcgcgtga gttctttgat gcggtgccag ctctggcgcg
     3301 taagctgcaa acgttgatgg acgttggtct gacgtacatt cgcctggggc agtccgcaac
     3361 cacactttct ggtggtgaag cccagcgcgt gaagctggcg cgtgagctgt caaaacgcgg
     3421 caccgggcag acgctgtata ttctcgacga gccgaccacc ggtctgcact tcgccgatat
     3481 tcagcaactc ctcgacgtgc tgcataaact gcgcgatcag ggcaatacca ttgtggtgat
     3541 tgagcacaat ctcgacgtga tcaaaaccgc tgactggatt gtcgacctgg gaccagaagg
     3601 cggcagtggc ggcggcgaaa tcctcgtctc cggtacgcca gaaaccgtcg cggagtgcga
     3661 agcttcgcat acggcacgct tcctcaagcc gatgctgtaa tcgttaaggc cgctttctga
     3721 gcggcctttt cctttcagag ttgtaccagc aatttacgct tttcttccgg tagtaaattc
     3781 actgcctgct gataagacgc atccaccaga taatagattt gcgaatccgg cagcgaaccg
     3841 tcgagataca cggtgctcca gtgcgcttta ttcagatggc ggctaggacg cacatcgctg
     3901 tgctgctgac gtagcagctc cgccagctcc gggctggttt tcagcgaaac agctgggcga
     3961 ttttctactt ctttcaccat cgcaaacagt acatcttcca ctttgatctg cgtcgctttc
     4021 cagtcgttat gcacgctctg ttctgcgctt ggttttgcca tgcaatattg tagcaactcc
     4081 gaaatggtca ttttttactc cccttgtagt gtcgcgatga tgcgacgcga tccgccgtgg
     4141 atgcgatgtt cccccagcca gatgccttgc caagtgccgg tctgaatacg ccctttatgt
     4201 accggcaata caagcgatgt tcccagcatt gaggatttga tatgagaagg catatcgtct
     4261 gctccctcat agtcatgctc ataatttccg ttgtcgggaa cggtgcggag gaaaaaacac
     4321 tccatgtcgt ggcgtacggt gggatcgcag ttctcattaa gtgtcagaga ggcggaggta
     4381 tgttgcagca acagatgcag taagccgatg ttaacgcgcg gcatatcagc cagctgattc
     4441 agaatttcat ccgttaccag atgaaaccca cgagatttgg cgctaagcgt gagcgtcttt
     4501 tgataccaca tgtgctgctc cttgataaaa ctctcttaat cagtttgcag caagacagcg
     4561 aaaggataaa ggtgtgatta aaaaaacagc attcgggaga gcatcactct ctcccgctct
     4621 gtcagtattc tgaattgacg atcacctctt caccaaacgc acccgcttgt ggcaagggtt
     4681 tgtaggtaga gttggaggcg cgcagaatgc ggataccacg agcgccgaca tcgcgtgcgg
     4741 cggtaatatc gttatcggaa tcgccataaa aaattcggat atttttatcc tgcagccatt
     4801 gcgattttgt attttgccct ggtttatcgc ccgcaaagat taccggattc atgttggtgg
     4861 caggaatatg aaaattatcc gccagcgttt ttgaaaccgt ttctgttttc gtcgggctac
     4921 gaccagtcac aaagaagatc gcgtcaccgc ggcgtacatg catatcaatc agctggcgag
     4981 cgacctcttt tggaatgctg aattcatccc agccattatt cattttttcc cagaacacag
     5041 gatttttcag ataatcttcg ctttctggcg agaaggtttt tttgccgcgc cagaaccccg
     5101 gactggaaaa aagtaccgtg tcatcgatat caaaccccac cgccattggc ggacgccctg
     5161 cgaggctatt ttcaatttgt gcgaccgaaa cccaatgaat tggtgcctgt tcagcaagcc
     5221 tggcaacgtt agtcccaggg ttaagcggtg aaggagatga ggccagggca acagcggaac
     5281 tgtttagagc gaacaataag caaacggcac tgattgcctg tgtgatcttg cgcatatttt
     5341 tccctaaata gtcagtatgt tgaaactttt tattgtgaga tttgttgcaa aaaccatctg
     5401 accataacgc cagctgccgg ggaaaggaag gattatctgc ggtttttgtg atgaaaagca
     5461 ggatgattat atttaaccca gtaaatacgt tcctgtcttt ttttgtataa aaaatgttta
     5521 ttactcgatg taaggataca ttaattttac gtttattatg atgaatcctc ggcaaatgag
     5581 cgaaatttga tagaacattt atctgctgta cgatttaatg aacttaatga tacagcatta
     5641 caaaaacaat cgaagtttat aaagatgatt tctgattgac cagcccccta ccctacaatg
     5701 gtgattaatt ttattactcg cgatggagat gatgatatga atatatcata tgtgaatagc
     5761 aacaaaacga catctttacc tgttgagcta gatgcactaa ataataaaga tatctcttat
     5821 gccaaagatt tctttttata catagaaaca cagttaaaaa ttgcaaaaga tttttgtaga
     5881 cctggagaag aagtatcaag ttctattgca agtaaagttt ttcacgcatt tattgattta
     5941 gttaacaaaa tcaggggtaa gaaagacttt atgtatattt gcacgctttg ctgttttgcc
     6001 gaggaggtta aaggtgatta ttctcattac aggacctttt tatttgatat tggtaatcaa
     6061 tataaggtta aacttacaca aagcggaaaa aagagttctc tttaacatta gaatttaacg
     6121 atactataat tgaatctcag aaagtcacag gcaataaggc aaagcatatt cttgaggata
     6181 tagaaatatt ctatcgtaac aaacccgata cttattatta aatttaataa tggttagatg
     6241 tgcagaaatt attactccac acgaaaaaat agggaaagga attccagata attgggataa
     6301 acttaacaat aaaagaactg acaacgtttt attatctctc acgtagaaca acggcatcaa
     6361 aaaaatcatc tacaaagaaa tagtgaaagc gacgacagtt taatttcact gcaggctggg
     6421 taactccagc ctgctttcct gcattacatc accgcagcaa acgcctttgc cacacgttgt
     6481 acatttgccg tatttaaccc ggcgacacac atgcgaccgc tggcgatgag atagacacca
     6541 aattcttcac gtagtcggtc aacctgagcg gcacttaaac cggtataact gaacatgccg
     6601 cgctgattaa gcagataatc gaaattgcgt tctggcatct ctgtgcttaa taccttcacc
     6661 aattcctgac gcattgccag aatgcgagta cgcatctctt ctacttccgc cagccagctg
     6721 gctttcaatg cctcgtcatt cagcactgca gccaccacct gcgcaccaaa attcggcggg
     6781 ctggagtagt tgcggcgaac tgttgctttc aattgcccca gtacgcggcc agcggcttcg
     6841 gcatcttcac acagaacaga aagtccgccg acgcgctcgc cgtaaaggga gaaaattttc
     6901 gagaacgaat tgctcaccag agcgggtaat ccagcactgg caatggcgcg aatagcgtag
     6961 gcatcctctt ccataccggc actaaatcct tgataggcaa tatcgaggaa tgggataagc
     7021 tcgcgggctt tgagaatttc aatcaccgca tcccactgat cattagtgag atcggcaccc
     7081 gttgggttgt ggcaacatgg atgcagcaac acaatactgc gggcaggtaa tgttttcagc
     7141 gtcgccaaca ggtcattaaa gcgcacgctg ttagtcactt cgtcatacca ggggtaagta
     7201 ctcacttcga atccagcccc ggcgaatatt gctacgtggt tttcccaagt aggatcgctg
     7261 acccagacgc ctgattccgg gaagtagcgt ttcaggaaat ccgcgcccac tttcaatgcc
     7321 cctgagccgc caagggtttg aatggttgct acgcgctgtt gttgcagtac cggatggtcc
     7381 gcaccaaaca gcagcggcgc aatggcatgg cgatagctgt taagcccttc catcggtaaa
     7441 taaagcgaag cgccatgggg ctgcgcattc aggcgcgctt ccgcctccgc cacggctttc
     7501 aattgtggaa taattccgtc ttcgttgtag tacagaccga tacttaaatt cactttgtcg
     7561 ctgcgagggt cttctttaaa acgctccata agcgtaagaa tcgggtcgcc agcgtaggcg
     7621 tcaacttttt gaaacacgcg atggttctcc aggtttacgg gcaggtggtt aaaacacaat
     7681 aaaccggaag aaggcgaaga tcgagtggat gttcaggagc gaacggcaat tagcaacaga
     7741 gtgagactaa tgataaacgt acatccgcca gaggcacgac ctttataaga gcatgaaaga
     7801 acatcaactt attgaatttt taggatttta ttggccggat aaggcattca cgccgcatcc
     7861 ggcacagaca atcaaatatt acagaacgat taatccacgt atttcatcgc gacccttgaa
     7921 gtcaggcgcg taataagttc gtaagcgctt actttcgtca tttcagcgat acgttctacg
     7981 ggcaaacctt cgccccataa aatgaccgga tccccggctt tgtcctgcgc ctgtggacct
     8041 aagtctacgc agatcatatc catcgccacg cgcccgacaa tcggtacttc gcgaccgttc
     8101 accagcactg gcgtaccgga cggcgcggcg cgcggataac catcgccata gcccatcgcg
     8161 actacgccaa gacgggtatc acgttcgctt acccaggttc caccataacc aacaggctct
     8221 ccggctttat gctcacgcac ggcaatcagg ctggaggtta gtgacatcac tggctgacag
     8281 ccaaaatcgg caccggtgga gcgatcttcc agcggcgaga cgccataaag aatgatgccc
     8341 gggcgcaccc agtcaaaatg cgactgtggc cacagcagaa tgccacccga cgcggcaatg
     8401 gaacgttgac caggtttgcc ttcgcaaaag gtattaaaga tagcgagttg tttctcggtt
     8461 gcgccacatt ttggttcatc cgcgcgcgca aaatggctga cgatattcac cggctgacga
     8521 acgtttttgc actgggtcag gcgatgataa aacgcctcag cctgttccgg ccttacgccc
     8581 agacggtgca taccggtatc gagtttcatc cagacggtaa ccggctcgtc caagctagcc
     8641 tcttccagcg cagccagctg ttcttcgtta tgcacggcgg tatgaaaatg ttgcgcagaa
     8701 atcgtcggca gatctctggc atcaaaaaag ccttcgagta acagtacagg tttggtgatt
     8761 ccccccgcac gcagtcgcag agcttcttcg agacgggcta cgccaaaggc gtcagcatcg
     8821 gggagcgttc gcgcggtctc aagaagaccg tgaccataag cgttcgcttt caccaccgca
     8881 accattttac tggcaggtgc cagttcacga agacgttgca ggttgtgtcg cagagcgcgg
     8941 cggttaatca caacagttgc cgcttgcatt tgtgttcctt gataattgtt tgctttaatt
     9001 acctaattca taaaataatt attattcgtc gtcgtactgc ggccccgcat agttgtcgaa
     9061 gcgcgaccat tgaccgttaa aggtcaggcg taccgtcccg attgggccgt tacgttgttt
     9121 accgataata atttccgcga tgccttttaa atcactgttt tcgtgataca cctcatcacg
     9181 atagataaac atgatcaagt ccgcatcctg ctcgatagag ccagattcac gcaggtcgga
     9241 gttgaccgga cgtttgtcgg cacgttgttc cagagaacgg ttcaactggg acagcgccac
     9301 caccggcacg ttcagttctt tcgccagtgc tttcagcgag cgggagattt ctgcaatttc
     9361 cagcgtacgg ttatcggaaa gcgccggtac gcgcatcagt tgcaggtagt cgatcatgat
     9421 aagcccgatg ccgccgtgtt cacgggcaat acggcgtgcg cgggaacgca cttccgttgg
     9481 cgtcaggccg gaagagtcat cgatatagat attgcgtttt tcgagcaaaa tacccatggt
     9541 gccggaaatg cgcgcccagt cttcatcatc gagctgcccg gtacggattt tagtctggtc
     9601 aacgcgcgac agcgacgcca gagaacgcat catgatctgt tctgatggca tctccagcga
     9661 gaagataagt actggtttat cctgcaacat tgccgcgttt tcgacgaggt tcatcgcaaa
     9721 tgttgtttta cccatcgacg gacgcgcggc gacgatgatc aaatccgacg gctgcaagcc
     9781 agcggttttt ttgttgagat cgtcataacc ggtgtttacc ccggtaacgc catcgtgtgg
     9841 ctgctgaaac aactgctcaa tacgcgccac ggttgcgtcg agcacatcgg cgatgttctt
     9901 cggcccttcg tctttgtttg cacgactttc ggcaatttta aagacgcggg attcagcaag
     9961 gtccagcaga tcttcgctgg tacgcccctg cggatcaaaa ccggcttcag caatctcatt
    10021 cgcaaccgag atcatctcac ggacaacggc acgttcacgc acgatgtccg cataagcact
    10081 gatgttcgcc gcacttggcg tattttttga cagctctgcc agataagcaa aaccaccgac
    10141 gctatcgagt tgcccctggc gttccaacga ttccgcaagg gtaatcagat cgataggact
    10201 accgctttcc tgcaaacgcg ccatttcagt aaagatatga cggtgtgggc gggtgtaaaa
    10261 atcgtctgct accacacgct cggctacatc atcccagcgt tcgttatcca gcattaaacc
    10321 gcccaacacc gactgctccg cttcgatcga gtgcggaggc actttcagcc cggcaacttg
    10381 tggatcgcgt tcgcggggtt cagcctgctg tttgttgaag ggtttatttc ctgccatagt
    10441 gaatggagtt accgaaataa agaatgggtc gaaactttac catatgaagc agaccctgac
    10501 gatacgttct ggaggacaca tggcaacacg aattgaattt cacaagcacg gtggcccgga
    10561 agtacttcaa gccgtagagt tcactcctgc cgatccggcg gagaatgaaa tccaggtcga
    10621 aaataaagcc atcggcatca attttatcga cacgtatatc cgcagcggcc tttacccgcc
    10681 gccatcgcta cccagcggat taggtaccga agcggcaggc atcgtgagta aagtcggcag
    10741 tggtgtaaag catattaagg ctggcgatca tgtagtctat gcgcagtcgg cgttaggcgc
    10801 ttacagctct gtgcataaca ttaatgcgga taaagcggcg attctgcctg cggcaatttc
    10861 ttttgagcaa gctgcggcat ccttcctgaa aggcttaacg gtttattatc tgctgcgcaa
    10921 aacctatgaa attaaacccg atgaacagtt cctgttccac gcagcggctg gcggcattgg
    10981 cttaattgcc tgccagtggg caaaagccct gggcgcgaaa ctcatcggca ccgtaggaac
    11041 cgcgcaaaaa gcgcagagcg cgctaaaagc cggcgcgtgg caggttatta actatcgtga
    11101 agagaatctg gtcgagcgat tgaaagagat caccggcggt aagaaagtgc gcgtagtgta
    11161 cgattccgtg ggcagagaca cctgggaacg gtcgctggat tgcctgcaac gccgcggctt
    11221 aatggtcagt tttggcaact catcaggtgc ggttaccggt gtgaacttag gcattctcaa
    11281 tcaaaaaggc tcgttgtatg tgacacgccc ttccctgcaa ggctatatca ccacgcggga
    11341 ggaattaacc gaggccagta atgaactgtt ctctttgatt gccagcggtg tgattaaggt
    11401 cgatgtcgcc gagcagcaga aatatccgct gaaggatgcg cagcgtgcgc atgagattct
    11461 ggaaagccgg gcgacgcaag gttccagcct gctgattcca taaaagaaat agggcttcca
    11521 cctgggaagc cctttctttt tatagttcgg ctgtatgtag ggtacagcac gatgaatctg
    11581 ttagaggcgc aatagtgaca gatttgatta tcaattccta ttttgttcta aggataaaac
    11641 cttaggttgt gatcatccgc acaatccctt agtaacgcca gcggtcataa cgctgatatt
    11701 tcggcatttt tggtgcttta atcgccttaa taacccacac caccgcaatc gccagtagta
    11761 accacggcag caacttaatc atcaatgcca gcataccgcc gaggaacata atggccgtcg
    11821 ccacaaccag cgcggcgata atgcccagca acgaaacgcc ggtgaccatc agcatgacaa
    11881 aaaagccaat cacaaaaagt agttccagca tgatgctctc ccaaatatga aatctcttgc
    11941 tggcattaca agaatcatgc caaaaataat ctattgattt aacagcaaaa cgccccgcga
    12001 cggtgcgcag ggcgtggtga atttgactac tttttggtga aaagttaacg cttatccgcc
    12061 accagtttga gcgcgtgttc cagcacgtta atgtcagcac ccgctttatg ggcattttca
    12121 cttaaataac gccgccactg ccgcgcgcca ggaataccct ggaacaaacc cagcatatgc
    12181 cggataatat ggccgagata cgtcccctgg ctgagttcat gctcaatgta cggatacatg
    12241 gcacgcacta ctgccaccgg atcggcatcg gtatccgagg aaccaaagat ctcccggtct
    12301 accgccgcca gaatacccgg attctgatac gcctcgcgcc cgaccatcac gccatccata
    12361 tgttgcaaat gcgctttagc ttcttccagc gacttgatac caccgttaat cgacattgtc
    12421 agatgcggga agtcacgctt cagttgatac acacgcggat aatcgagcgg cgggatttca
    12481 cggttttctt tcgggcttaa ccccgaaagc caggctttac gtgcgtggat gatgaacatc
    12541 tcacactcgc ctttgccgga aacggtattg atgaaatcgc agagaaattc atagctgtcc
    12601 tggtcgtcga tgccaatacg cgttttcacc gtcaccggaa tcgacaccac atcgcgcatc
    12661 gctttcacgc agtcggcaac cagctgcgca ttacccatca gacacgcacc aaacatgccg
    12721 ttctgcaccc ggtcagacgg gcagccgaca ttcaggttga tctcatcata tccgcgcgct
    12781 tctgccagct ttgcacactg cgccagcgcc gccggatcgc taccgcccag ttgcaacgct
    12841 accggatgtt cttcttcact gtacgccaga taatcacctt taccgtgaat tatcgcccct
    12901 gtggtcacca tttcggtata aagcaacgta ttgcgggaaa gcagacgcaa gaaatagcgg
    12961 caatgtctgt ccgtccagtc gagcattggt gcaacactaa accgaccact ccagtgaacg
    13021 tcagtttttt caggcattgc gctggtttgg ttgatttttt gcatttcaga attaccgtgc
    13081 attttcaaat gtagagatta ttttatcgat atatcatggg gttatgttat tcagcttcac
    13141 tgttcaggag gctcaatagc ggggtactat accataacaa caggaagcgc ctgtctcatt
    13201 gcaaaagaaa attgagatcc tctcaaggca tgaagctctc acgaagtgat ggaaataatc
    13261 ttattagccg ttaactttgt taaggccaat gataaacaat ccaggttcga cgataaataa
    13321 aaaatcacac attaaactct ggtgatatat ctccctgcta atagcattga tagagaaaaa
    13381 agaacccaat aaatattggg tccttttata taatgccttc catactatcg aagaacttca
    13441 catattattt ctccgattta accccgaaca agtcataaat taatttagaa gtgtctgtaa
    13501 gtattttaat ctcttccttt gaggttgggt caaacgactt cgcgaagcta attaattgtg
    13561 gtgcagcatc tcgcattttg cttaaaatat caggatcgag cgttccttca ttcaccagat
    13621 gtgacatctt atccagatag ccatcaaaat tcatttctcc cccatctgcc agacgcttca
    13681 tctctcccag gtacttcttc atatcacgtt gatataattt ttcaaactga gctttcaggt
    13741 agctctcatt attttcatta atatacactg tttctgataa atctgcgtag gcactagaat
    13801 atgaaagtga aattaacagc gaagcaaata attttaagct gttgtcatca tgttcctggc
    13861 aggcattaac aaacgttaaa a
//
LOCUS       DAJTSI010000105        13618 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000105 DAJTSI010000000
VERSION     DAJTSI010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13618
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.105"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OO327_002827"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OO327_002827"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR6019294.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OO327_002828"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OO327_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR6019295.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OO327_002829"
     CDS             complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OO327_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR6019296.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3807..3995
                     /locus_tag="OO327_002830"
     CDS             3807..3995
                     /locus_tag="OO327_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR6019297.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4007..4759
                     /gene="bcsQ"
                     /locus_tag="OO327_002831"
     CDS             4007..4759
                     /gene="bcsQ"
                     /locus_tag="OO327_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR6019298.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            4756..5115
                     /locus_tag="OO327_002832"
     CDS             4756..5115
                     /locus_tag="OO327_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR6019299.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            5112..7373
                     /gene="bcsA"
                     /locus_tag="OO327_002833"
                     /pseudo
     CDS             5112..7373
                     /gene="bcsA"
                     /locus_tag="OO327_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            7384..9723
                     /gene="bcsB"
                     /locus_tag="OO327_002834"
     CDS             7384..9723
                     /gene="bcsB"
                     /locus_tag="OO327_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCR6019300.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            9730..10836
                     /gene="bcsZ"
                     /locus_tag="OO327_002835"
     CDS             9730..10836
                     /gene="bcsZ"
                     /locus_tag="OO327_002835"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR6019301.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            10913..11254
                     /locus_tag="OO327_002836"
     CDS             10913..11254
                     /locus_tag="OO327_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR6019302.1"
                     /translation="MDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRRSIDDIER
                     SLQNDRLAQQAEVLENQGKWAQAAALQRQRLALGPGSVWITYRLAGRSCAAIQRWRQQ
                     SGLRLHGAGIT"
     gene            11169..11420
                     /locus_tag="OO327_002837"
                     /pseudo
     CDS             11169..11420
                     /locus_tag="OO327_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCSC C-terminal domain-containing protein"
     gene            11502..13490
                     /gene="hmsP"
                     /locus_tag="OO327_002838"
     CDS             11502..13490
                     /gene="hmsP"
                     /locus_tag="OO327_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR6019303.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
BASE COUNT         3195 a         3474 c         3716 g         3233 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcttggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      841 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaaatc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1021 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccagg
     1321 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aggcaaccag atgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtaac gcggaatggg catcagcaga
     1621 aacgcggcaa acaccaaatt gaggagcggg tggaagttaa gatatcccgc ccacaacagg
     1681 ccaaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gataataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcattcccc attgttctgg
     2101 ggcaagcagg cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacatagcgt cagggcttgc tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggc aagagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggtacatag cgactaaact tctgcccttg
     2521 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2581 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3001 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctcaa gcaacaatga
     3061 aaattgttta tcgttattat ttccgggatt aattaccaaa agcgaacaat ggtttaacct
     3121 gctccattta ttcattttat ccaaccatga gcgaagccgc tcggcaggaa tattttgcca
     3181 tgcgttattt gcgcaaacaa gaataaaaag gtaattatgg ggatcaatag aacactgcaa
     3241 atcacgggcc aaatagtata gacctttttc atgatttagc attgaaaata attttatttt
     3301 ttccggccct tgagaatcat ctaattgaaa gattttcgcc ggatcgctgt ccatgctaat
     3361 gaccgcgacg tgtgcggttt cagcctggga tgcaattgtt tgattcgcca gactgatagc
     3421 atcttcatgg cgatcgacgt taaaccacca gacgccgcct gctggcatat gtcgcagctc
     3481 atcccataat gatgagatac cgatagagaa tacagggtcc acaatgtccc tcatgtcacc
     3541 attttgtctg attatcagtt tactagcgaa agctaagaaa taaacctacc attgaaatta
     3601 aaacttatca gatttagcat gtgaatcaat ttagaaggtc acaagacgtg tgattttagt
     3661 cttttcgttt ttaacttttt ctgttgcgta acggaaagtc aaaaagtgag caaattcccg
     3721 tctcgccgta aacaataaac cggcgaaatg ctcacggtta gaattgtctc atgaatggta
     3781 cggttatttc atagggatca agcaaaatga ataacaatga accagatact ctgcctgatc
     3841 ccgcgatagg ctatatcttc cagaatgata ttgtggcgtt aaagcaggca ttttcactgc
     3901 ctgatattga ttatgccgat atttcccaac gcgaacagtt ggccgcggca ttaaaacgct
     3961 ggccgttgct ggcagagttt gcgcaacaaa agtaggggat tggtgaatgg ccgtactggg
     4021 attgcagggg gtgcggggag gcgtggggac aacaaccatc accgccgcat tagcctggtc
     4081 attacaaatg ttgggagaaa atgtcctggt ggtcgatgcc tgcccggaca acttgttgcg
     4141 cctgtcattt aatgttgatt ttacccaccg tcagggctgg gccagagcga tgctggatgg
     4201 ccaggactgg cgtgacgctg ggttgcgcta cacctcgcag ctcgatttgc tgccttttgg
     4261 tcagttatcc attgaagaac aagaaaatcc acagcactgg caaacccggc tgagcgatat
     4321 ttgctccggc ttacagcaac taaaagccag cgggcgttac cagtggattt taatcgactt
     4381 accgcgtgat gcctcgcaga taacccacca gctgctgagt ttgtgcgagc actcgctggc
     4441 aatcgtcaat gtggatgcca actgccatat ccgactgcat cagcaagcgc tgccggatgg
     4501 cgcacatatt ttgattaatg acttccgtat tggcagtcag gttcaggacg atatttacca
     4561 gctttggttg caaagccagc gccgattact gccgatgctc attcatcgtg atgaagcgat
     4621 ggctgaatgc ctggcggcta agcaaccagt aggtgaatat cgcagtgatg cgctggcggc
     4681 tgaagagata ctgacgctgg cgaactggtg cctgttgaac tactccgggc tgaaaacgcc
     4741 agtcgggagt gcatcatgag tatcctgacc cggtggttgc ttatcccgcc ggtcaacgcg
     4801 cggcttatcg ggcgttatcg cgattatcgt cgtcacggtg cgtcggcttt cagcgcgacg
     4861 ctcggctgtt tctggatgat cctggcctgg atttttattc cgctggagca cccgcgctgg
     4921 cagcgtattc gcgcagaaca taaaaacctg tatacgcata tcaacgcctc gcgtccgcgt
     4981 ccgctggacc cggtccgtta tctcattcaa acatgctggt tattgatcgg tgcatcgcgc
     5041 aaagaaacgc cgaaaccgcg cagcgggcat tttcaggtct gcaaaatatt cgtggacgtt
     5101 accatcaatg gatgaacgag ctgcctgagc gcgttagcca taaaacacag catctggatg
     5161 aaaaaaaaga gctcggtcat ttgagtgccg gggcgcggcg gttgatcctc ggtatcatcg
     5221 tcaccttctc gctgattctg gcgttaatct gcgttactca gccgtttaac ccgctggcgc
     5281 agtttatctt cctgatgctg ctgtgggggg tagcgctgat cgtacggcgg atgccggggc
     5341 gcttctcggc gctaatgttg attgtgctgt cgctgaccgt ttcttgccgt tatatctggt
     5401 ggcgttacac ctctacgctg aactgggacg atccggtcag cctggtgtgc gggcttattc
     5461 tgctcttcgc tgaaacgtac gcgtggattg tgctggtgct cggctacttc caggtagtat
     5521 ggccgctgaa tcgtcagccg gtgccattgc cgaaggatat gtcgctgtgg ccgtcagtgg
     5581 atatctttgt cccgacttac aacgaagatc tcaatgtggt gaaaaatacc atttacgcct
     5641 cgctgggtat cgactggccg aaagacaaac tgaacatctg gatcctcgat gacggcggca
     5701 gggaagagtt tcgccagttt gcgcaaaacg tgggggtgaa atatatcgcc cgcaccactc
     5761 atgaacatgc gaaagcgggc aacatcaaca atgcgctgaa atatgccaaa ggcgagttcg
     5821 tgtcgatttt cgactgcgac cacgtaccaa cgcgatcgtt cctgcaaatg accatgggct
     5881 ggttcctgaa agagaaacag ctggcgatga tgcagactcc acaccacttc ttctcgccgg
     5941 acccgtttga acgcaacctg ggacgttttc gtaaaacgcc gaacgaaggc acgctgttct
     6001 atggtctggt gcaggatggc aacgatatgt gggacgccac tttcttctgc ggttcctgtg
     6061 cggtgattcg tcgtaagccg ctggatgaaa ttggcggcat tgctgtcgaa accgtgactg
     6121 aagatgcgca tacttctctg cgtttgcacc gtcgtggcta tacctccgcg tatatgcgta
     6181 ttccgcaggc ggcggggctg gcgaccgaaa gtctgtcggc gcatatcggt cagcgtattc
     6241 gctgggcgcg cgggatggta taaatcttcc gtctcgataa cccgctcacc ggtaaaggac
     6301 tgaagtttgc tcagcggctg tgctacgtca acgccatgtt ccacttcttg tcgggcattc
     6361 cacggctgat cttcctgact gcgccgctgg cgttcctgct gcttcatgcc tacatcatct
     6421 atgcgccagc gttgatgatc gccctattcg tgctgccgca tatgatccat gccagcctga
     6481 ccaactcgaa gatccagggc aaatatcgcc actctttctg gagtgaaatc tacgaaacgg
     6541 tgctggcgtg gtatatcgca ccaccgacgc tggtggcgct gattaacccg cacaaaggca
     6601 aatttaacgt caccgccaaa ggtggactgg tggaagaaga gtacgtcgac tgggtgatct
     6661 cgcggcccta catcttcctt gttctactca acctggtggg cgttgcggta ggcatctggc
     6721 gctacttcta tggcccgcca accgagatgc tcaccgtggt cgtcagtatg gtgtgggtgt
     6781 tctacaacct gattgttctt ggcggcgcag ttgcggtatc ggtagaaagc aaacaggtac
     6841 gccgatcgaa ccgcgtggag atgacgatgc ccgcggcaat tgcccgcgaa gatggtcacc
     6901 tcttctcgtg taccgttcag gatttctccg acggtggttt ggggatcaag atcaacggtc
     6961 aggcgcagat tctggaaggg cagaaagtga atctgttgct taaacgcggt cagcaggaat
     7021 acgtcttccc gacccaggtg gcgcgcgtga tgggtaatga agttgggctg aaattaatgc
     7081 cgctcaccac ccagcaacat atcgattttg tgcagtgtac gtttgcccgt gcggatacat
     7141 gggcgctctg gcaggacagc tacccggaag ataagccgct ggaaagtctg ctggatattc
     7201 tgaagctcgg cttccgtggc taccgccatc tggcggagtt tgcgccttct tcggtgaagg
     7261 gcatattccg tgtgctgact tctctggttt cctgggttgt atcgtttatt ccgcgccgcc
     7321 cggagcggag cgaaacggca caaccatcgg atcaggcttt ggctcaacaa tgatgataac
     7381 gcgatgaaaa gaaaactatt ctggatttgt gcagtggcta tggggatgag tgcgttcccc
     7441 tctttcatga cgcaggcgac gccagcaacg caaccactga tcaatgctga gccagctgta
     7501 gccgcccaga cggaacaaaa tccgcaggtg gggcaagtga tgtcaggcga gcagggagct
     7561 gatgccccca tcgtggcgca gaacggtcct tcgcgtgatg tgaagctgac ctttgcgcaa
     7621 atcgcgccgc cgccgggcag catggtgcta cgtggtatta acccgaacgg cagcattgag
     7681 tttggtatgc gcagcgatga agtggtgacg aaggcgatgc tcaacttcga atacacgcca
     7741 tcgccatcgt tactgcctgt ccagtcgcag ttaaaggttt atctcaatga tgagctgatg
     7801 ggcgtgctgc cagtgaccaa agaacagttg ggtaaaaaaa cgctggcgca aatgcccatt
     7861 aatccactgt ttattaccga cttcaaccgt gtgcggctgg agtttgtcgg ccattatcag
     7921 gacgtgtgcg aaaacccggc cagcaccacg ctttggctgg atgttgggcg gagcagtgga
     7981 ctggatctga cctatcagac cctgaatgtg aagaatgacc tgtcacactt cccggtgcca
     8041 ttctttgacc cgcgcgataa ccgtactaac accttgccga tggtctttgc gggtgcgccg
     8101 gatgttgggc tgcaacaagc atctgccatt gtcgcctcgt ggtttggttc gcgttctggc
     8161 tggcgtgggc agaacttccc ggtgctctat aaccaattgc cggatcgcaa tgccattgtc
     8221 tttgcaacca acgacaaacg gccggacttc ctgcgcgatc atccggcggt aaaagccccg
     8281 gtgattgaga tgattaacca tccgcagaat ccttacgtca aactgctggt ggtgtttggt
     8341 cgtgacgaca aagacctgtt gcaggcagcg aaaggtatcg ctcagggtaa cattctgttc
     8401 cgtggtgaaa gcgtggtagt gaatgaagtg aaaccgctgc taccgcgtaa gccgtacgat
     8461 gcgccgaact gggtacgtac cgatcgtccg gtcacatttg gcgaactgaa aacctatgaa
     8521 gaacagttac aatccagcgg ccttgagcca gcagcgatta acgtttcgct aaacctgccg
     8581 ccggatctct acctgatgcg cagtaccggc attgatatgg atattaatta ccgctacacc
     8641 atgccgccgg tgaaagacag ttcgcggatg gatatcagcc tgaataacca gttcctgcaa
     8701 tccttcaacc tgagcagcaa acaggaggcg aaccgtctgc tgctgcgtat tccggtatta
     8761 caaggtttgc tggatggcaa aacagatgtc tctattccgg cgctgaaact gggcgcgacc
     8821 aaccagctgc gcttcgactt tgagtatatg aacccgatgc caggcggttc ggtagataac
     8881 tgtattacct tccagccggt gcagaatcat gtggtgattg gtgacgattc caccatcgac
     8941 ttctcgaagt attaccactt catcccgatg ccggatctac gcgcctttgc taacgcgggc
     9001 ttcccattca gccggatggc ggatctgtcg caaaccatca ccgtgatgcc gaaagcgcct
     9061 aacgaagcac agatggaaac gttgctgaat actgttggtt ttatcggcgc acagacgggc
     9121 ttcccggcga ttaatctgac ggtgaccgat gatggcagca ccattcaggg caaagatgcc
     9181 gacatcatga tcatcggtgg tatcccggac aaactgaaag acgataagca gatcgaccta
     9241 ttggtgcagg cgaccgaaag ctgggtgaaa acaccgatgc gccagacccc gttccccggc
     9301 attgtgccgg acgagagcga tcgcgcggca gaaacccagt caacgctgac ctcttccggt
     9361 gcgatggcgg cggtgattgg cttccagtcg ccgtataacg accagcgcag cgtgattgcg
     9421 ctgttggcag atagcccacg cggttatgaa atgcttaacg atgcggtgaa cgatagcggc
     9481 aaacgcgcca ccatgttcgg ttcggtcgcg gtgatccgcg agtccggtat caacagccta
     9541 cgtgttggcg acgtttatta cgtaggtcat ctgccgtggt tcgagcgctt gtggtatgcg
     9601 ctggcaaacc atccgattct gctggcggtg ctggcgacta tcagtgtgat attgctggca
     9661 tgggtactgt ggcgtctgct gcgaattatt agtcgtcgtc gtcttaaccc ggataacgag
     9721 taattgaaga tgaatgtgtt gcgtagtgga atcgtgacga tgctgttgtt ggctgccttt
     9781 agtgttcagg cagcctgtac ctggcctgcc tgggagcagt ttaaaaagga ttacatcagt
     9841 caggaagggc gcgtcatcga ccccagcgac gcgcgcaaaa tcaccacctc cgaagggcaa
     9901 agttacggta tgttctttgc cctggcggct aacgaccgtg cagctttcga taatattctc
     9961 gactggacgc agaacaatct cgctcagggt tctttaaaag aacgtttgcc cgcctggctg
    10021 tggggcaaga aagagaacag taagtgggaa gtgctggaca gcaattcggc ctccgatggt
    10081 gatgtctgga tggcctggtc gttgctggag gcggggcgtt tgtggaaaga gcagcgttat
    10141 accgacatcg gcagcgcgtt gctaaaacgt atcgcgcggg aggaagtggt gacggtgcct
    10201 gggctgggtt ccatgttgtt accgggcaaa gtgggttttg ctgaggataa cagctggcgt
    10261 tttaacccca gctacatgcc gccgacgctg gcgcagtatt tcacccgctt tggcgcgccg
    10321 tggaccacgc tgcgcgaaac caatcaacat ttattgctgg aaaccgcccc gaaaggcttt
    10381 tcgccagact gggtgcgcta tgagaaagac aaaggctggc agctaaaagc cgaaaaaaca
    10441 ttgatcagca gctacgacgc tatccgcgtt tacatgtggg taggcatgat gcctgacagc
    10501 gatccgcaaa aagcgcggat gctcaaccgg tttaaaccga tggcgacatt cactgagaaa
    10561 aacggttatc cgccggaaaa agtggatgtg gctacgggga aagcgcaggg taaaggaccg
    10621 gtcggttttt ctgccgccat gctgcctttt ttacaaaacc gcgatgcgca ggcagttcag
    10681 cgccagcgcg tggccgataa ctttcccggc agcgatacct attacaacta tgtgctgacc
    10741 ctgtttggac aaggctggga tcaacaccgt ttccgcttct cgacaaaagg tgagttatta
    10801 cctgactggg gccaggaatg cgcaaattca cactaaacat attcacgctt tccctcggtc
    10861 tggccgtcat gccgatggtc gaggcagcac caaccatcag cagaccctgc ggatggacag
    10921 cggcaacact aatgccgtgc gcgggctggc gaatatttac cgccagcagt cgccagaaaa
    10981 agctgaagcg tttatcgcct cgctctctgc cagccagcgg cgcagcattg atgatatcga
    11041 acgcagcctg caaaacgacc gtctggcaca gcaggcagag gtactggaaa accagggcaa
    11101 atgggcgcag gcggcagcac ttcagcggca acgactggcg ctgggccccg gcagcgtatg
    11161 gattacttac cgactggcag gaagaagctg cgcggcaatc caacgatggc ggcagcagtc
    11221 agggcttcgg ctacacggcg cgggcattac ttgaacgacg tgttacttcc aactggtttg
    11281 ttggcacggc aattgatatc cagcaggtga aagattacgc acccagccat ttcctgctct
    11341 acgtacgtta ttccgccgcc ggatggcagg gtgacatgga tttaccgccg cagccgctga
    11401 taccttacgc cgactggtaa gttttcagat agcgcctctc ttaatgccgc tgcgatcggg
    11461 tatactcggg cggcaatctg ggatttccgg ggggagacaa tttgcgcgta agtcgctcgt
    11521 taacaatcaa gcagatggca atggtggcag ccgttgtcct ggtgttcgtt tttatttttt
    11581 gcaccgtttt gctgttccat ctggtccagc agaatcgcta taacacggct acgcaactgg
    11641 aaagcattgc tcgctctgtc cgcgaaccct tatcttcagc tattttgaaa ggcgatattc
    11701 ccgaagcgga agctattctt gccagcatta aaccggcagg cgtggtcagc cgtgccgatg
    11761 tagtgctgcc taaccagttc caggcgctgc gtaaaagttt tattccagag cgtccggtgc
    11821 cggtaatggt tactcgcctg tttgagctac cggttcaaat ctcgctgggc gtctactcgc
    11881 tcgaacgtcc ggcaaatccg cagccaatag cctatctggt gctacaggcg gattccttcc
    11941 gtatgtataa gttcgtgatg agcactctct caacgttagt gaccatttac ttacttttgt
    12001 cgcttatcct gaccgtcgcc atcagctggt gcattaaccg cctgattttg catccgttac
    12061 gcaatattgc tcgcgaactt aacgccatcc cagcccagga gcttgttggt caccaactgg
    12121 cattaccgcg tctgcatcag gacgatgaaa tcggtatgtt ggtgcgcagt tacaacctca
    12181 accagcaatt gctgcagcgc cattatgaag aacagaacga aaatgcgatg cgcttcccgg
    12241 tgtcggattt gccgaacaaa gccttgctga tggagatgct ggagcaggtt gtcgcgcgta
    12301 aacaaaccac cgcgctgatg atcatcacct gtgaaaccct gcgtgatact gcgggcgtgc
    12361 tgaaagaggc gcaacgagaa attctgctgc tgacgctggt ggaaaaactc aaatcggtac
    12421 tgtcgccacg tatgatcctc gcgcagatta gcggttatga ctttgctgtc attgccaacg
    12481 gtgtacagga accgtggcac gcaatcactt tgggtcagca agtactcact atcatgagcg
    12541 agcgcctgcc gattgaacgt attcaactcc gtccgcactg tagcattggc gtggcgatgt
    12601 tctacggcga tctcaccgcc gaacagcttt acagtcgcgc tatttctgcg gcatttaccg
    12661 ctcgccataa aggcaagaat cagattcagt tctttgatcc gcagcagatg gaagcagccc
    12721 agaagcggtt gacggaagag agcgatatcc ttaatgcact ggaaaatcat cagtttgcaa
    12781 tttggttaca gccacaggtc gagatgacca gcggtaaact ggtcagtgcg gaagtgttac
    12841 tgcgtatcca gcaaccggat ggcagttggg acctgccgga tggcttaatc gatcgcattg
    12901 agtgctgtgg gctgatggtt accgtcggtc actgggtgct ggaagagtcc tgtcgattgc
    12961 ttgcagcctg gcaagagcgc ggcattatgc tgcccttgtc ggtaaacctc tctgcgctgc
    13021 aactgatgca cccgaatatg gtggcggata tgctggaact gttaacccgc tatcgcattc
    13081 agccgggaac actgattctg gaagtgacag aaagccgacg tattgacgac cctcatgctg
    13141 cggtggcaat cctccgtccg ctgcgcaatg ccggagttcg ggtggcgctg gatgatttcg
    13201 gcatgggcta cgcagggctg cgtcagctgc agcatatgaa atcgttgcca atcgacgtac
    13261 tgaaaatcga caaaatgttt gttgaaggct tgccggaaga tagcagcatg attgctgcaa
    13321 ttatcatgct ggcgcagagc ctgaacttac aaatgattgc cgaaggcgtg gagactgaag
    13381 cacaacgaga ctggctggca aaagcgggcg ttggtattgc ccagggcttc ctttttgctc
    13441 gcccactccc tattgaaatc ttcgaagaga gttacctgga agaaaagtag ctaccccaaa
    13501 ctgattacaa aactttaaaa agtgctggtt tgtgcgagcc agctcaaact ttttaacctt
    13561 tttgtttcaa ttatgatcca ggtacatttc tgtgatgttg tctgggtgtt attttaag
//
LOCUS       DAJTSI010000106        13611 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000106 DAJTSI010000000
VERSION     DAJTSI010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13611
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.106"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(172..1197)
                     /gene="tdh"
                     /locus_tag="OO327_002839"
     CDS             complement(172..1197)
                     /gene="tdh"
                     /locus_tag="OO327_002839"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR6019304.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="OO327_002840"
     CDS             complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="OO327_002840"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR6019305.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="OO327_002841"
     CDS             complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="OO327_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HCR6019306.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKRKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3839..4771
                     /gene="rfaD"
                     /locus_tag="OO327_002842"
     CDS             3839..4771
                     /gene="rfaD"
                     /locus_tag="OO327_002842"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR6019307.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4781..5827
                     /gene="rfaF"
                     /locus_tag="OO327_002843"
     CDS             4781..5827
                     /gene="rfaF"
                     /locus_tag="OO327_002843"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR6019308.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5831..6823
                     /gene="rfaC"
                     /locus_tag="OO327_002844"
     CDS             5831..6823
                     /gene="rfaC"
                     /locus_tag="OO327_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR6019309.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6820..8028
                     /gene="rfaL"
                     /locus_tag="OO327_002845"
     CDS             6820..8028
                     /gene="rfaL"
                     /locus_tag="OO327_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCR6019310.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8064..9206)
                     /locus_tag="OO327_002846"
     CDS             complement(8064..9206)
                     /locus_tag="OO327_002846"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HCR6019311.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9215..10228)
                     /locus_tag="OO327_002847"
     CDS             complement(9215..10228)
                     /locus_tag="OO327_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HCR6019312.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTVIKYNV
                     MKG"
     gene            complement(10253..10960)
                     /gene="rfaY"
                     /locus_tag="OO327_002848"
     CDS             complement(10253..10960)
                     /gene="rfaY"
                     /locus_tag="OO327_002848"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR6019313.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10986..11993)
                     /gene="waaO"
                     /locus_tag="OO327_002849"
                     /gene_synonym="rfaI"
     CDS             complement(10986..11993)
                     /gene="waaO"
                     /locus_tag="OO327_002849"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR6019314.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHIFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(12036..12833)
                     /gene="rfaP"
                     /locus_tag="OO327_002850"
     CDS             complement(12036..12833)
                     /gene="rfaP"
                     /locus_tag="OO327_002850"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR6019315.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12826..>13611)
                     /locus_tag="OO327_002851"
     CDS             complement(12826..>13611)
                     /locus_tag="OO327_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR6019316.1"
                     /translation="FYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQT
                     EAERFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSI
                     EALASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADL
                     LLHPAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEIL
                     RKALTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
BASE COUNT         3874 a         3034 c         2777 g         3926 t
ORIGIN      
        1 tgtgagatat tatttttaca ggtgagagag tcacttcagg gtagagagat aatgagaaca
       61 gctatataaa agatcagcgc aattacgaac tctttattac actgacagga ttttacataa
      121 ttaacagatt atcctcagat aaaagggctg gaataccagc ccttgttcgt gttaatccca
      181 gctcagaata actttcccgg actggcccga acgcatagcg tcaaagccct tctggaaatc
      241 atcgatagag aaacgatggg taatgatcgg cgagagatcg aggccagact gaatcagcgc
      301 cgccatcttg taccaggttt caaacatctc acgaccgtaa atgcctttaa tgaacaagcc
      361 tttaaagatc actttggtcc agtcgataga catatcagac ggtggaatac ccagcatcgc
      421 aatacgaccg ccgtggttca tggtatcgag catggtacga aacgctggcg gcgcgccgga
      481 catttccaga ccgacatcaa agccttcggt catgcccagt tcagccatca catcattaag
      541 gttttctttc gcgacgttaa ccgcacgggt gatacccatt ttgcgcgcca gctcaaggcg
      601 gtattcgtta acatcggtga tcaccacatt gcgtgcacca acgtgtttcg ccaccgccgc
      661 agccataata cctatcggac ctgcaccaga aaccagcaca tcttcgccca ccagatcgaa
      721 cgacagcgcg gtatgcacgg cgttaccgaa gggatcaaaa atggaagcca ggtcgtcgga
      781 gatattgtcg gggattttga aagcgttgaa cgccggaatc accagatatt cggcgaagca
      841 gcccggacgg ttaacaccga caccaatggt gttgcggcac agatgggtac gaccgccacg
      901 acagttgcga cagtgaccgc aggtgatatg gccttcgcca gaaacgcgat cgccgatttt
      961 gaagcctttc acttcctgac caatacctac cacttcaccg acatattcat ggcccacgac
     1021 catcggcacc gggatggttt tttgcgacca ctcatcccag ttatagatgt gaacgtcagt
     1081 cccgcagatg gctgttttac ggattttaat cagcagatcg ttatgcccga gttccggtac
     1141 agggacgtcg gtcatccaga tgccctcttc cgctttcagt ttggataacg ctttcatctc
     1201 acatcctcag gcgataacgc ccagttgttt accaatacgc gtgaatgctt ctactgcacg
     1261 cgtaatttgc tcaggggtat gcgccgcaga catctgggta cgaatacgcg cctgaccttt
     1321 cggaacgacc ggatagaaga aaccggtgac gtaaatgccc tctttttgca gctctcgggc
     1381 aaatttctgc gccactaccg catcaccaag catgaccgga ataatggcgt gatcggctcc
     1441 cgccagggta aagcccgccg ccgacatttg ctcacggaac tgacgcgcgt tcgcccacag
     1501 acggtcacgc aactcgctac ccgcttcaac catctccagc actttgatgg aagcggcgac
     1561 aattgctggt gccagcgagt tggagaacag gtacggacga gaacgctggc gcagccactc
     1621 aaccacttct ttgcgcgccg cggtataacc accagaagcc ccgcccagcg ctttaccgag
     1681 cgtgccggtg atgatgtcga cacgccccat cacatcgcag tattcatggg aaccacgacc
     1741 attttcaccg acaaaaccga ccgcgtggga gtcgtctacc atcaccaggg catcgtattt
     1801 atctgccaga tcgcaaacgc ccttcagatt ggcaatcacg ccgtccatcg agaacacacc
     1861 atcggtggca atcagcacat gacgcgcacc ggcttcacgc gcttctttca gacgagcttc
     1921 aagctcctgc atatcgttgt tggcatagcg atagcgttta gctttacaca gacgcacacc
     1981 atcaataata gacgcgtggt tcagtgcgtc ggagataatg gcgtcttccg cgcccagcag
     2041 cgtttcaaac aggccaccgt tagcatcaaa gcaggaagag tagagaatcg catcttccat
     2101 ccccaggaat gccgccagtt tttgttcaag ctctttatgg ctgtcctgag tgccgcaaat
     2161 aaaacgcacc gaagccatgc cgaaaccgtg agaatccatt cccgcctttg ctgccgcaat
     2221 cagatccgga tgattcgcca gcccgagata gttgttggca caaaagttaa tgacgtggct
     2281 tccatcagcc acggtgatat ctgcttgctg cgcagacgta ataatgcgct cttctttaaa
     2341 caacccttcc gcccgtgcgg tttccagatc gttggttaac tgctgataaa attctccacg
     2401 cattgcgatt ctccagacag ggcaaattcc agcacatatt acccaaactt ataggtcgcg
     2461 acgagatgat gcgttaacac ttctgcaaaa ttcaggataa ataacgtgta tcttgatgtc
     2521 tctcaacacc tacagatagt agaaaaactg tacagttttg gctgagcata atgatgagtg
     2581 attttactgc attgatataa aaagaaaaaa tgatgccaaa gcatcatttt tcctatctca
     2641 gttggcatca aaacgctacc cactaatgga gggaaaccta ttttcttgaa aggaaaattc
     2701 tcagcgcctg catttttgcc cctaaagtgt tactcctgaa gcagcgaaac aaatcgaacc
     2761 attttcctct ccccagatag tttaggttga agagatcgga acgtttgtaa tagcacatga
     2821 caaaaatgcc ctggtcatcg tccacaatgt tattgtttag tgttattttt tgactttcca
     2881 gaactaattt atagaattct ttccatttat gctgtgaacc aacaattgcg cccccaataa
     2941 tatatacatg attgccaatc atgaaatcaa aagcctgttg ttgagatgta acagtaagcc
     3001 ctttttttat agtgaaaaga tgcatcttat tttcatcaaa tgggaaatcc caaattttca
     3061 atcctcgagt tacatttggc ttacggcaat agccaaaatc tatccacgca acaagaggcg
     3121 ttttcactaa acccatgttg atagctttat ttacaaaata ggccttaagg ttacatacca
     3181 aaacatactc tggtgaccag tactctgggt tttttaattg tcgaggctca agtctatttg
     3241 taaatgactc atctttttga attttttcga tacgacttct gatatatcta aatttccttt
     3301 ttatatcgat aacgataacc gttgttggtt ttccgttgcg aatcgcttca atccttggtt
     3361 tcaaatcggg agaagtgaaa ataatcatct cattttcaag tgcggccaga cgctcaaagt
     3421 aactaaaata aacatcaact gaacgagcta gtttttcacg gaacccttta ttagcagtcc
     3481 aatcccctct accaatgtca aaatatgcag tgataattgt ggtggatgat ttcattacta
     3541 ataagaccat taatataaaa aaagaattat atcataaata ttaacagaat gtatactgta
     3601 tattatccat tcaaatgatt ccgttattta tcatacatcg gaatattgat actaaagcac
     3661 tattcacatg caaaaccaac atccgccatg aaggactagc taaaacccaa actagtttgt
     3721 tgcaattagc atccttgcac ctctatgtaa agggctgaag ggattcggat gtgatggtat
     3781 gattacagac attcgtgtct gagattgtct ctgactccat aattcgaagg ttacagttat
     3841 gatcatcgtt accggcggcg cgggctttat cggcagcaac atcgttaagg ccctgaatga
     3901 taaaggcatc accgatattc tggtggtgga caacctgaaa gacggcacca agtttgtgaa
     3961 cctggtggat ctggatatcg cggactatat ggataaggaa gacttcctga tccagattat
     4021 ggctggcgaa gagttcggtg atgtcgaagc gattttccac gaaggcgcat gctcttccac
     4081 caccgagtgg gatggcaagt atatgatgga taacaactat caatactcca aagagctgct
     4141 gcactactgt ctggagcgcg aaatcccgtt cctgtatgcc tcttccgcag ccacctacgg
     4201 cggacgcacc tccgacttta ttgaatcccg cgagtacgaa aaaccgttga atgtctacgg
     4261 ttactcaaaa ttcctgtttg atgaatatgt tcgtcaaatc ctgccagaag cgaactcgca
     4321 gattgttggc ttccgctatt tcaacgttta tggaccgcgt gaaggccata aaggcagcat
     4381 ggcgagcgtc gctttccatc tcaacaccca gcttaataac ggtgaatcac cgaagctgtt
     4441 cgaaggtagc gagaacttca aacgtgactt cgtctatgtg ggcgacgtgg cagatgtaaa
     4501 cctgtggttc ctggaaaatg gcgtttccgg tatcttcaac ctcggcaccg gtcgtgcaga
     4561 atccttccag gcagtagcag atgctacgct ggcttatcac aagaaaggcc agatcgaata
     4621 cattccgttc ccggataagc tgaaaggccg ctaccaggcg ttcactcagg cagatctgac
     4681 aaatctgcgc gcggcgggtt acgacaaacc gttcaaaacc gttgctgaag gtgtaacgga
     4741 atacatggcc tggctgaatc gcgacgcata agagctctgc atgaaaatac tggtgatcgg
     4801 cccgtcttgg gttggcgaca tgatgatgtc gcaaagtctc tatcgcacgc tccaggcgcg
     4861 ctatccccag gcgataatcg atgtgatggc accggcatgg tgccgtccat tattatcgcg
     4921 gatgccggaa gttaacgaag ctatccctat gcctctcggt cacggagcgc tggaaatcgg
     4981 cgaacgccgc aaactgggtc atagcctgcg tgaaaagcgc tacgaccgcg cctacgtctt
     5041 accaaactcc ttcaaatctg cattagtgcc tttcttcgcg ggtattcctc atcgcaccgg
     5101 ctggcgcggc gagatgcgct acggtttact caacgatgta cgcgtgctcg ataaagaagc
     5161 ctggccgcta atggtggaac gctatgtcgc gctggcctat gacaaaggca ttatgcgtac
     5221 cgcacaagat ctgccgcagc cattgttatg gccgcagttg caggtgagcg aaggtgaaaa
     5281 atcatatacc tgtaatcaat tttcgctttc atcagaacgt ccgatgattg gcttttgccc
     5341 gggtgcggag tttggtccgg caaaacgctg gccacactac cactatgcgg aactggcaaa
     5401 gcagctgatt gatgaaggtt atcaggtggt tctgtttggc tctgcgaaag atcatgaagc
     5461 gggcaatgag attcttgccg ctttgaatac cgagcagcag gcatggtgtc ggaacctggc
     5521 gggggaaaca cagcttgatc aagcggttat cttgattgca gcctgtaaag ccattgtcac
     5581 taacgattct ggcctgatgc acgttgcggc ggcgctcaat cgtccgctgg ttgccctgta
     5641 tggtccaagt agcccggact tcacaccgcc gctatcccat aaagcgcgcg tgatccgtct
     5701 gattaccggc tatcacaaag tgcgtaaagg tgacgctgcg gagggttatc accagagctt
     5761 gatcgacatt actccccagc gcgtactgga agaactcaac gcgctattgt tacaagagga
     5821 agcctgacgg atgcgggttt tgatcgttaa aacatcgtcg atgggcgatg ttctccatac
     5881 gttgcccgca ctcactgatg cccagcaggc aatcccaggg attaagtttg actgggtggt
     5941 ggaagaaggg ttcgcacaga ttccttcctg gcacgctgcc gttgagcgag ttattcctgt
     6001 ggcaatacgt cgctggcgta aagcctggtt ctcggccccc ataaaagctg aacgcaaagc
     6061 gtttcgtgaa gcgctacaag cagagaacta tgacgcagtt atcgacgctc aggggctggt
     6121 aaaaagcgcg gcactggtga cacgtctggc gcatggcgta aagcatggat tggactggca
     6181 aaccgctcgc gaacctttag ccagcctgtt ttacaattgt aagcatcata ttgcaaaaca
     6241 gcagcacgcc gtagaacgca cccgcgaact gtttgccaaa agtttgggct atagcaaacc
     6301 gcaaacccag ggcgattatg ctatcgcaca gcattttctg acgaacctgc ctacagatgc
     6361 tggcgaatat gccgtatttc ttcatgcgac gacccgtgat gataaacact ggccggaaga
     6421 acactggcga gaattgattg gtttactggc tgattcagga atacggatta aacttccgtg
     6481 gggcgcgccg catgaggaag aacgggcgaa acgactggcg gaaggatttg cttatgttga
     6541 agtattgccg aagatgagtc tggaaggcgt tgcccgcgtg ctggccgggg ctaaatttgt
     6601 agtgtcggtg gatacggggt taagccattt aacggcggca ctggatagac ccaatatcac
     6661 ggtttatgga ccaaccgatc cgggattaat tggtgggtat gggaagaatc aagtagagtg
     6721 tcgttctaca agtatgtctc ttgcagattt gccagctcaa accgtttttc aaaatttaaa
     6781 tcttgaaata ataaccaata agttgacatc ggagataaga tgacctcaac attatttttc
     6841 tctctcgaga aaaaaaactg gatagcgtac tggaacagag ctctcgtatt cttattcatt
     6901 accacctatt ttttgggtgg gattacaagg tataaacatc ttattgttat tcttatgaca
     6961 ataacgacaa tcgtctatct ctgcaaacgg ccaaaacact atctctcact atttaaaaca
     7021 tttctttttg gtagtgttgc catattaact attgctgcat tgctgtcact tcttcaatcc
     7081 cctgatgcag gtgctagcat gaaggaagtt ttcaaagcta ttattgagaa tactttacta
     7141 tgcacaatag caataccggt catattgaga gacgagaaaa gagaagatgt cgaaaaaatc
     7201 gttttcttct catttattag tgcgttgggc ttacgctgtt tttctgaatt gattacctat
     7261 tataaggact atcaacaagg gataatgcca ttcgcagatt atagacaccg tagcatttct
     7321 gactcgatgg tctttttatt ccctgcattg ttaaatctct ggcttatcaa atcagcaaaa
     7381 taccgcattt cttttgtggt tctaagcgtt atttttattt ttctgatatt aggaacttta
     7441 tccagagggg cctggctttc cgtgttagtc attggattaa tatggattct gatgtttaaa
     7501 caatggaagt tactattagt aggagtaatg gtttccatca ttgcattgtc ggttattttc
     7561 acacataagg agatgaccgc aaagctaacg tataaacttc aacaaactaa tagttcttat
     7621 cgctatgcaa atggtactca aggcagcgca ctcgatctaa tattagaaaa tcctgttatt
     7681 ggttatggtt acggtaacgt tgcatataaa gatgtctata ataaacgtgt cattgattat
     7741 ccagaatgga cctttagaca atcaataggg ccacataatt ttgcgctatt catctggttt
     7801 ggcactggtt tattagggct ggtaagtctt atgatgctat actgtgcaat attgaaagag
     7861 tgtataaaaa atggcgtcaa gaataaatat cgctcaccat ataatgcata ttatataatc
     7921 ttactatctt ttataggtta ttttgttatc cgtggaaacg tagaacaaat tgaaccaaat
     7981 ttattaggcg tttacgccgg cttattatta gcgatgaaaa acaagtaaaa aataaaaaag
     8041 gctgcataat gcggcctttt tatttattca aaccaattat gaataacctc ttcgaatctt
     8101 tgagttacgc catcccaact gtagtgatca aacacaaagt cctgaccttg ctttgcaact
     8161 gctgttaatt caggattcgc taacgttttt aaaatatcgc tgcttatgga gtctgcagtc
     8221 ataggttctt ttaaatggaa tcccgtagta ttttctttga caaattccgt catcccccca
     8281 cgcgtactca ctaaaacagg cttaccagca cccatagctt caattgccac catgcaaaat
     8341 ggttcctgga actgggaagg aatgacgact aaatctgcca gtgggtaata acaatacatc
     8401 ttttcaggcg gtatgcttcc taacataatg caacgatcct tcaaacgttt tgcaatttcc
     8461 ctgacattgc gttgatatgc acctttgtca cttttactca tttctgtata atcaccaatc
     8521 acaactaatt ttaaattttt gtgagcagcg gccaattttt cgaatgattg catcaacagc
     8581 aatatgcctt tgtcagggac gatacgccca gcataaaaaa taattttctc ttcaggagag
     8641 atattaagtt cgcttctagt gatcggctga aaattagatt gataggtttc taaatcaatt
     8701 ccatttggta caatctcaat atctgcattt gctaaataag attggtaata ctttttcaag
     8761 tataaactag ggactatcat tttaacattt tgctcaagca attttggttc aaatgcatta
     8821 tgcatatgta taaccactct tgcctgagga gctagcttac gaatttgagt gtatagcttc
     8881 atgctattat gaactacaat aacactttct ttcgttatag gaaaatcatg tgcaatattc
     8941 aatattctct gtgaatatgg aagaggatca agtcttgtcc atttttgaaa taaccgctta
     9001 tatatgcgac tgaatccaac cctatgaata ctacaattat cattaacaaa ggtgtaattt
     9061 gagtatcctg gatttttgat acaaacaata cgatttggaa aattggtacg ctgagcaacc
     9121 tgatacatcc acgtttcaac agcagccgct ccgcgaggag gaattgaaaa aataggagta
     9181 accgtaaata ttattttatc aaccatctcg tatattaacc tttcataaca ttatatttaa
     9241 ttactgtaaa aacaccatcg agaaattttt tctggtaaag caagtgttta tatttttctt
     9301 tgtgctcatg ttttttaaca gcttttttat aaggtatatt tctccatggt gatatattat
     9361 aaatattacg aaaataatct gcagaggcgt agttcgccca atcatgccat ggcttagtta
     9421 taccagtata atgtatgaag acagtgtcat cattaataaa ccgggtgtaa tattcactat
     9481 ttttttcttc aaattctgac tttatagtat aaatacaatt atattttctc ggtaatattt
     9541 tagcatgatg tagaaacatt aagtttaaaa tatcttgatc cgggtacttt aattttttta
     9601 taattgactc atctgataat agatcaaaga atttttccgt tagtctttgt tttaaccatt
     9661 cccgtaaatt aatatacatt acaccagagt taaaatagct accgttaaaa tcctcattac
     9721 acaaacgctc agcgctttta ctttgcatag catctacatc aattaccact gccccatatt
     9781 catctttaaa ctctaatgct attaactcgt tcaatgaacc cttacagacg atatcagcat
     9841 caagatataa taatgaatcc aatcgcgcag aaaaataatc aaatgaaagc aaacgataat
     9901 aaatagatac tggccagata tttgattgag gtagcgcctt caatggctca gaatcaatat
     9961 gatataattt aattactgtt tgatacttcg ttgctaattg tgataataat tcaatatact
    10021 ctttgtctaa gtaatcagcg attatgtgaa aggtaaattt gttttgttta ttcttttcaa
    10081 gaattgaagc aattgaaatc gcagccccaa cctggtagtt ctcatcaata ccccatgaga
    10141 cattaaacgt ttcccgttca tcaagtttaa cggggcgctt gtccagttca attatatctt
    10201 taaattgagt aagatgttta aaatccaatt taattaccct tcatcataat cattatttgc
    10261 tcttcaatcc tagtttgact tttacatccc tgattacttt tttaattttc tttttgaata
    10321 taagatatgt atagccaaag tctttcaatt cattttttat attatagtgc cgttcaagat
    10381 caatgcgatc ttttgctttt aaaacagcgg ttgttttttt acctgaaagg tcaattagtc
    10441 ttaaaccttt ttcggatact ataaaatttc ctttatgagg atcgccagac accattccat
    10501 gctgatgcaa ctcttttatc gattctgata attgatcctt cagatcttca gaaatttcca
    10561 aatactcatt taacccgaca ccttcaatat actcaatgag cataatataa taatgagcga
    10621 aatttaatgt tttacgttca gctagcaaaa aatagtcatt aattgattgg atgccttcac
    10681 cacgtacccg atcggtttga taaattaaat tttcataata atcttttttt atgcaagatt
    10741 ttaagaaacg ttcggtcatt ttatgctttg gcgcataaac tttaagtaca agaggtccac
    10801 gagcagtgtc aataagaatg actttagtat catcaataga acgaaaaact tttaatgttt
    10861 taaccctgca agttaaaaag tcatttagaa cttgctcata aaagggatca ttatctttta
    10921 tgaaaacttt caacccattt atcgttttat tatatatcat aatgaattat tttaacctta
    10981 aatctttaga agcatttttc tttataatac tttaaataat taaaaatgcc tgccatataa
    11041 tgcttttgtt taaatttatg ttttgcacaa taccgatact gattgctatt tacaggttta
    11101 agtaaatctt ctttactcca cggagaagct gctttggcga tcaaaaaact acgtgaaacc
    11161 ggataatcag cccactcatg ccagggtttt gtaggtccca catagtgtat aaacacggtg
    11221 tcatcattga ctggattatc aactttgtct tttaattcat agttaatact atatcgggta
    11281 ttatatttct ccgaaataaa cttcacttta ccattcaata atacattcag tacatcttga
    11341 tcaaggtggg tgattttact tacccaatct gggtcacgca acatttcaat agcttttgac
    11401 gaaatgttat ttagattcca ctcatcaata tttatcagta aaaaaccagc attgaaatat
    11461 ccagaagcta actgtggtgt agttaatacc gaggctcgat tttgccacca ttcaatatct
    11521 ctttcagcta caactgcggc aatttcatta gtagaaaatt gataatctaa gagttcttta
    11581 atactaccct tgcaagcaat atctgcatca agatatagta ttttttcatg tttatgatag
    11641 aaataatctg caattataaa tcgaaaatat gttgcgtaag tccagttttt cgtgcttggt
    11701 aatgacttca gcttatcaca attgataaca taaatattaa ttcttgaatt atattgtttt
    11761 gctaaatcag aaaaatataa tttgtcttta tcactgatat aatctgtgaa aatatgaaat
    11821 tcacaagaga tttctctatt gtttaataga atagatgcga tggctacacc acatccaaaa
    11881 agaaaatttc tgtcaatacc aaatgctata tcaagagtat ttttagatct tatattttga
    11941 taatgaaaat tatactcaaa aaggatgatg tcactatcat tgagttgaga catatttatt
    12001 ctgtatccaa aattttggca aaaataagtt acgggttaca acgattttcg aatcgttctt
    12061 tccctgattt ttgtggcttt tgcttctgct tgcgacagca gtccttgttc ctgcttgaga
    12121 atgtctttaa gcggggcggc aaaatacacc ttcataaagc gccagatatc ccgctgagtc
    12181 aggccaatat tcattgaaga aaaataaagc cctataagat ctttatcgcg ccaacgacct
    12241 ggaacgcgcg tgcgaagctg cgcccggtgt aggtcaatta ccgaaatttt taactcctct
    12301 tccttaccgg aaaaaggcaa gtgcagcagg aaatgacaga tataacagtc acgatggtta
    12361 atgcccgcag catgcatatc gcgcaccatc gtcgcgacac gcttaataag catacgcttt
    12421 acgcgaacat ctggtgggtt ggtcgcccag tcagcacagt aatcttccag actgatggtt
    12481 ggtgtcagat cttcggtaat aataaacgag gttctggtca gcggattcat gcctttttcg
    12541 ccgaatgcca ccccatacat agtatcaacg ccgacatccc gcagtcgatg aatcgcattc
    12601 cattcgcggt ctgcgcctaa tactggcatc cgcaatgaga gtaaattttt gattatctct
    12661 ttcagggtcg tgccgcgatg ccatttgaga aaatagcttt ttcccgccat ttcaaagcgc
    12721 agagtacggc gagtttccag ttcacgaaat acctcaccct gcaaggtttt aacttcctca
    12781 aaaggatctt tgccgcgcca taacgtggca aacggctctt taagttcaac catccagacc
    12841 acccgttatg atatccgccg ctttctctgg cagactgtat aaatcttgtg tatcagcata
    12901 atgtcgcgca ttttccgccc aagcctggcg caatgaagat tgcgttaacg ctttgcgtaa
    12961 aatctcattc aatgtttcct ggcggaatgg ctcagcaata gcctcgccgc aattagcgtc
    13021 gacaatataa tgcgcatagc cacaaacggc agttgttagt accggtaatc ctgcagtaat
    13081 cgcttccagc agcacaattc ccgccgcttc ctggtaggca ggatgcagta ataaatccgc
    13141 cgccgccatt aattccgaga catcgttgcg ccccgagaag aagtgaacat tactgcgcac
    13201 gccgcgtttt tctgccagtg cctcaaattt tcgcggttta tcctgcccaa caacatatag
    13261 caatgtgttg tggcgcagcg aatccggtaa cgaagcaagt gcttcaatgg aacgatcggc
    13321 acctttacgc gtgaagtctg aaccgacctg caacaataaa tattgttgtt cggttattcc
    13381 attcttctta cggaagattt cacggctatt ggctggctgc tggctatatt tacgatcagg
    13441 ataaatcccc ggtggcagaa tatgaaaacg ctccgcttca gtctgataat gtttctggaa
    13501 atcggcgatt tgcttatctg tcagcatcag cagctgtgtc ggcttgccct gttcgaaggt
    13561 tgcccgctca aaggcggcat aatggcgata acgtgacgtc aggcgataga a
//
LOCUS       DAJTSI010000107        13248 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000107 DAJTSI010000000
VERSION     DAJTSI010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.107"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(16..998)
                     /gene="kdgT"
                     /locus_tag="OO327_002852"
                     /pseudo
     CDS             complement(16..998)
                     /gene="kdgT"
                     /locus_tag="OO327_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
     gene            complement(1258..1878)
                     /gene="sodA"
                     /locus_tag="OO327_002853"
     CDS             complement(1258..1878)
                     /gene="sodA"
                     /locus_tag="OO327_002853"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR6019317.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2163..3197
                     /gene="rhaT"
                     /locus_tag="OO327_002854"
                     /pseudo
     CDS             2163..3197
                     /gene="rhaT"
                     /locus_tag="OO327_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3194..4060)
                     /gene="rhaR"
                     /locus_tag="OO327_002855"
     CDS             complement(3194..4060)
                     /gene="rhaR"
                     /locus_tag="OO327_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCR6019318.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(4116..4952)
                     /gene="rhaS"
                     /locus_tag="OO327_002856"
     CDS             complement(4116..4952)
                     /gene="rhaS"
                     /locus_tag="OO327_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR6019319.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5240..6709
                     /gene="rhaB"
                     /locus_tag="OO327_002857"
     CDS             5240..6709
                     /gene="rhaB"
                     /locus_tag="OO327_002857"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR6019320.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6706..7965
                     /gene="rhaA"
                     /locus_tag="OO327_002858"
     CDS             6706..7965
                     /gene="rhaA"
                     /locus_tag="OO327_002858"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR6019321.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QHHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8207..9031
                     /gene="rhaD"
                     /locus_tag="OO327_002859"
                     /pseudo
     CDS             8207..9031
                     /gene="rhaD"
                     /locus_tag="OO327_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            9041..9355
                     /gene="rhaM"
                     /locus_tag="OO327_002860"
     CDS             9041..9355
                     /gene="rhaM"
                     /locus_tag="OO327_002860"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR6019322.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            9656..10102
                     /locus_tag="OO327_002861"
     CDS             9656..10102
                     /locus_tag="OO327_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR6019323.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            10113..11564
                     /locus_tag="OO327_002862"
     CDS             10113..11564
                     /locus_tag="OO327_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCR6019324.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            11554..12624
                     /locus_tag="OO327_002863"
     CDS             11554..12624
                     /locus_tag="OO327_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR6019325.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            12624..>13248
                     /locus_tag="OO327_002864"
     CDS             12624..>13248
                     /locus_tag="OO327_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR6019326.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDEWPQ"
BASE COUNT         3158 a         3458 c         3491 g         3141 t
ORIGIN      
        1 attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgctg ctctggcttt
       61 gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgaccgc
      121 cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc
      181 aatcagcaca ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt
      241 gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat
      301 tactgccaca cccaacagga tccccagcaa accagtctgg gcaatcacag tcaaatcaat
      361 ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa
      421 ttctcgtaat tcagggtcaa gattccccag ggcaaagccc accaggaacg gtaatactgc
      481 gccgacgaaa acatgcggtt caaacgaggc aatcccggca gtgcccagaa taatcatcgt
      541 catgagcggc ccggactcca acgacatcaa cacaaatgcc cagcttcttc tttcgtaccg
      601 tactgctgca tgatggaagc gtaaagtccg ccgttggtca tatccatcgc cgccaccagc
      661 gccagcgttg aaagtccggc aaagaatcca acttcaacac catgttccgg aataatgcgc
      721 gaggcaattg ccgcaaccac ccacgcgacg gcaattttag ttaccaccag cgtaccggat
      781 ttacgcagta ccgttcccgt cgcgcttaat tttattgacg cccccatgca aaaaaaccac
      841 accgccagaa tgggcaccgt accggtaatc atcccgttgg tgaatgatcc aaaatatttc
      901 cccgcccccg gcgagaaggt gtggcacagt gcgccaagga atagcgggac gagcatcatc
      961 ccccccggga ttttctcaat cgagcgtttt atctgcattt ccatcacctg tcggcatatt
     1021 tttaagggct gatgcgtaat tcctcaatgg agcatcagtg ttgatggaaa cgaagataga
     1081 cattcattta atcaataaaa gtgatcaaaa tcacatcaat tgaaacgctg ttttattttt
     1141 ataggaccat tgtttttatt ttggaattga tcacaaaaaa accaccgccg ttggcgatgg
     1201 ttcatttttt aagctgatat gcggcctata cgcctcattg cagcaggcgg caaatgatta
     1261 ttttttcgcc gcaaaacgtg ccgctgcttc gtcccagttc accacgttcc agaactcttt
     1321 gatgtagtcc ggacggcggt tctggaattt caggtagtaa gcatgttccc acacatccag
     1381 gcccagaatc gggaagccgg aagcgccaga aatagcttca cccatcagcg gagaatcctg
     1441 gttagcagta gaaaccaccg ccagtttatc gcctttcagc accagccatg cccagccgga
     1501 accaaagcgg gaagctgccg ctttttcaaa ttctgctttg aagttatcaa cggagccgaa
     1561 gtcacgttcg atagccgctt tcaggtcacc ctgcagggtg gtgccttttt tcagaccttt
     1621 ccagaacagg ctgtggttag cgtgaccgcc agcgttgttg cgcagtacgg ttttcttgtc
     1681 tgctggcagc tggtccagtt tggtgatcag ctcttcaacc ggcaggttgg caaattctgg
     1741 caggctttcc agcgccgcgt tggcgttgtt tacgtaggtc tgatggtgtt tggtgtggtg
     1801 gatttccatg gtctgcttat cgaagtgcgg ttccagggca tcgtaagcat acggcaggga
     1861 tggcagggta tagctcatat tcatctccag tattgtcggg cggccgattg ttaatgccgc
     1921 gtaagcagtt ggttcattat agttaattaa atgatattga aaatgattat caatgccgta
     1981 cttttcgtaa gggtatggtt ttgcaggaaa atgcccgaga tgtgaagcaa atcacccact
     2041 taatgccgtg attgccagta aatcgacaac ggcggcaaca ggcgaaaggt taatcgacag
     2101 cacgattttt acactcatct cgtcggagat gtgaagcgac gaaaaatgat gaggataaga
     2161 agatgagtaa cgcgattacg atggggatat tttggcattt gatcggcgcg gccagtgcag
     2221 cctgttttta cgctccgttc aaaaaagtaa aaaaatggtc atgggaaacc atgtggtcag
     2281 tcggtgggat tgtttcgtgg attattctgc cgtgggccat cagcgccctg ttactaccgg
     2341 atttctgggc gtattacagc tcgtttagtc tctctacgct gctgcctgtt tttctgttcg
     2401 gcgctatgtg ggggatcggt aatatcaact atggcctgac catgcgttat ctcggcatgt
     2461 cgatgggaat tggcatcgcc attggcatta cgttgattgt cggtacgctg atgacgccaa
     2521 ttatcaacgg caatttcgat gtgctgatta acaccgaagg cggacgcatg acgttactcg
     2581 gcgttctggt ggcgctgatt ggcgtaggga ttgtaactcg agccgggcag ttgaaagagc
     2641 gcaagatggg cattaaagcc gaagagttca atctgaaaaa agggctggtg ctggcggtga
     2701 tgtgcggcat tttctctgcc gggatgtcct ttgcgatgaa cgccgcaaaa ccgatgcatg
     2761 aagccgctgc cgcacttggc gtcgatccac tgtatgttgc tctgccaagc tatgttgtca
     2821 tcatgggcgg cggcgcgatc attaacctcg gtttctgttt tattcgtctg gcaaaagtga
     2881 aggatttgtc gctaaaagcc gacttctcgc tggcaaaacc gctgatcatt cacaatgtgt
     2941 tactctcggc actgggcggg ttgatgtggt atctgcaatt ctttttctat gcctagggcc
     3001 acgcccgcat tccggcgcag tatgactaca tcagttggat gctgcatatg agtttctatg
     3061 tattgtgcgg cggtatcgtc gggctggtgc tgaaagagtg gaacaataca ggacgccgtc
     3121 cggtaacggt gttgagcctc ggttgtgtgg tgattattgt cgccgctaac atcgtcggca
     3181 tcggcatggc gaattaatct ttctgcgaat tgagatgacg ccactggctg ggcgtcatcc
     3241 cggtttcccg ggtaaacacc accgaaaaat agttactatc ttcaaagcca cattcggtcg
     3301 aaatatcact gattaacagg cggctatgct ggagaagata ttgcgcatga cacactctga
     3361 cctgccgcag atattgattg atggtcattc cagtctgctg gcgaaattgc tgacgcaaaa
     3421 cgcgctcact gcacgatgcc tcatcacaaa atttatccag cgcaaaggga cttttcaggc
     3481 tagccgccag ccgggtaatc agcttatcca gcaacgtttc gctggatgtt ggcggcaacg
     3541 aatcactggt gtaacgatgg cgattcagca acatcaccaa ctgcccgaac agcaactcag
     3601 ccatttcgtt agcaaacggc acatgctgac tactttcatg ctcaagctga ccgataacct
     3661 gccgcgcctg cgccatcccc acgctaccta agcgccagtg tggttgccct gcgctggcgc
     3721 taaatcccgg aatcgctccc tgccagtcaa gattcagctt cagccgctcc gggcaataaa
     3781 taatattctg caaaaccaga tcgttaacgg aagcgtagga gtgtttatcg tcagcatgaa
     3841 tataaaagag atcgccacgg gtaatgcgat aaggacgatc gttgagtaca tgcagaccat
     3901 taccgcgcca gacaatcacc agctcacaaa aatcatgtgt atgttcggca aagacatctt
     3961 gcggataacg gtcagccaca gcgactgcct gctggtcgct ggcaaaaaaa tcatctttga
     4021 gaagttttaa ctgatgcgcc accgtggcta cctcggccag agaacgaagt tgattattcg
     4081 caatatggcg tacaaatacg ttgagaagat tcgcgttatt gcagaaagcc atcccgtccc
     4141 tggcgaatat cacgcggtga ccagttaaac tctcggcgaa aaagcgtcga aaagtggtta
     4201 ctgtcgctga atccacagcg ataggcgatg tcagtgacgc tggcctcgct gtggcgtagt
     4261 agatgtcggg ctttcatcag tcgcaggcgg ttcaggtatc gctgaggcgt cagtcccgtt
     4321 tgctgcttaa gctgccgatg tagcgtacgc agtgaaagag aaaattgatc cgccacggca
     4381 tcccaattca cctcatcggc aaaatggtcc tccagccagg ccagaagcaa gttgagacgt
     4441 gatgcgctgt tttccaggtt ctcctgcaaa ctgcttttac gcagcaagag cagtaattgc
     4501 ataaacaaga tctcgcgact ggcggtcgag ggtaaatcat tttccccttc ctgctgttcc
     4561 atctgtgcaa ccagctgtcg cacctgctgc aatacgctgt ggttaacgcg ccagtgagac
     4621 ggatactgcc catccagctc ttgtggcagc aactgattca gcccggcgag aaactgaaat
     4681 cgatccggcg agcgatacag cacattggtc agacacagat tatcggtatg ttcatacaga
     4741 tgccgatcat gatcgcgtac gaaacagacc gtgccaccgg tgatggtata gggctgccca
     4801 ttaaacacat gaatacccgt gccatgttcg acaatcacaa tttcatgaaa atcatgatga
     4861 tgttcaggaa aatccgcctg cgggagccgg ggttctatcg ccacggacgc gttacaagac
     4921 ggaaaaaaat ccacactatg taatacggtc atactggcct cctgatgtcg tcaacacggc
     4981 gaaatagtaa tcacgaggtc aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa
     5041 aacgtcgatt tttcaagata cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac
     5101 cacaattcag caaattgtga acatcatcac gttcatcttt ccctggttgc caatggccca
     5161 ttttcctgtc agtaacgaga aggtcgcgaa ttcaggcgct ttttagactg gtcgtaatga
     5221 aattcagcag gatcacatta tgacctttcg caattgtgtc gccgtcgatc tcggcgcatc
     5281 cagtgggcgc gtgatgctgg cgcgttacga gcgtgaatgc cgcagcctga cgctgcgcga
     5341 aatccatcgt tttaacaatg ggctgcatag ccaaaacggt tatgtcacct gggatgtgga
     5401 tagcctggaa agtgccattc gccttggatt aaacaaggtg tgcgaggaag ggattcgtat
     5461 cgatagcatt gggattgata cctggggcgt ggactttgtg ctgctcgacc aacagggtca
     5521 gcgtgtgggc ctgcccgttg cttatcgcga tagccgcacc aatggcctga tgccgcaggc
     5581 acaacagcaa ctcggcaaac gcgatattta tcagcgtagc ggcatccagt ttctaccctt
     5641 caatacgctt tatcagttgc gtgcgctgac ggagcaacaa cctgaactta ttccacacat
     5701 tgctcacgct ctgctgatgc cggattactt cagttatcgc ctgaccggca agatgaactg
     5761 ggaatatacc aatgccacca ccacacaact ggtcaatatc aatagcgacg actgggacga
     5821 gtcgctactg gcgtggagcg gggccaacaa agcctggttt ggtcgcccga cgcatccggg
     5881 taatgtcatt gggcactgga tttgcccgca gggtaatgag attccggtgg tcgccgttgc
     5941 cagccatgat accgccagcg cggttatcgc ctcgccgtta aacggttcac gcgccgctta
     6001 tctctcttct ggcacctggt cattgatggg cttcgaaagc cagacgccat ttaccaatga
     6061 cacggcgctg gcagccaaca tcaccaatga aggcggggcg gaaggtcgct atcgggtgct
     6121 gaaaaatatt atgggcttat ggctgcttca gcgagtgctt caggaacggc aaatcaacga
     6181 tctcccggcg cttatcgccg cgacacaggc acttccggcc tgccgcttca tcatcaatcc
     6241 caatgacgat cgctttatta atcctgacga gatgtgcagc gaaattcagg ctgcgtgtcg
     6301 ggaaacggcg caaccgatcc cgggaagtga tgctgaactg gcgcgctgta ttttcgacag
     6361 tctggcgctg ctgtatgccg atgtgttgca tgagctggcg cagctgcgcg gtgaagattt
     6421 ctcgcaactg catattgtcg gcggcggctg ccagaacacg ctgctcaacc agctatgcgc
     6481 cgatgcctgc ggtattcggg tgatcgccgg gcctgttgaa gcctcaacgc tcggcaatat
     6541 cggcatcgag ttaatgacgc tggatgaact caacaatgtg gatgatttcc gtcaggtcgt
     6601 cagcaccacc gcgaatctga ccacctttac ccctaatcct gacagtgaaa ttgcccacta
     6661 tgtggcgcag attcactcta cacgacagac aaaggagctt tgcgcatgac cactcaactg
     6721 gaacaggcct gggagctggc gaaacagcgt ttcgcggcgg tggggattga tgtcgaggag
     6781 gcgctgcgcc aacttgatcg tttacccgtt tcaatgcact gctggcaggg cgatgatgtt
     6841 tccggttttg aaaacccgga aggttcgctg accgggggga ttcaggccac aggcaattat
     6901 ccgggcaaag cgcgtaatgc cagtgagcta cgtgccgatc tggaacaggc tatgcggctg
     6961 attccggggc cgaaacggct taatttacat gccatctatc tggaatcaga tacgccagtc
     7021 tcgcgcgacc agatcaaacc agagcacttc aaaaactggg ttgaatgggc gaaagccaat
     7081 cagctcggtc tggattttaa cccctcctgc ttttcgcatc cgctaagcgc cgatggcttt
     7141 acgctctcgc atcccgacga cagcattcgc cagttctgga ttgatcactg caaagccagc
     7201 cgtcgcgttt cggcctattt tggcgagcaa ctcggcacac catcggtgat gaacatctgg
     7261 atcccggatg gtatgaaaga tatcaccgtt gaccgtctcg ccccgcgtca gcgtctgctg
     7321 gcagcactgg atgaggtgat cagcgagaag ctaaaccctg cgcaccatat cgacgccgtt
     7381 gagagcaaat tgtttggcat tggcgcagag agctacacgg ttggctccaa tgagttttac
     7441 atggggtatg ccaccagccg ccagactgcg ctgtgcctgg acgccgggca cttccacccg
     7501 actgaagtga tttccgacaa gatttccgcc gccatgctgt atgtaccgca gttgctgctg
     7561 cacgtcagcc gtccggttcg ctgggacagc gatcacgtag tgctgctgga tgatgaaacc
     7621 caggcaattg ccagtgagat tgtgcgtcac gatctgtttg accgggtgca tatcggcctt
     7681 gacttcttcg atgcctctat caaccgcatt gccgcgtggg tcattggtac acgcaatatg
     7741 aaaaaagccc tgctgcgtgc gttgctggaa cctaccgctg agctgcgcaa gctggaagcg
     7801 gcgggcgatt acactgcgcg tctggcactg ctggaagagc agaaatcgtt gccgcggcag
     7861 gcggtctggg aaatgtattg ccaacatcac gatacgccag caggtagcga atggctggag
     7921 agcgtgcggg cttatgagaa agcgattttg agccagcgtg ggtaaacact gccggatgcg
     7981 gcacgagcgc cttatccggc ctacgggtcg aatgtccatg cgggctgtca ccctcacccc
     8041 ggccctctcc ctgaaagggc gagggagaaa aacatagcct cgtgcggcaa cggagctgaa
     8101 caccgctccg gacagtcccc tcgcccctac ggggagaggg ttagggtgag gggaacaagc
     8161 cgcaccatgt ctaaacaagg caacctaatt tacaggaaca cagaacatgc aaaacattac
     8221 ccagtcctgg tttgtccagg gaatgatcaa agccaccacc gatgcctagc tgaaaggctg
     8281 ggacgagcgc aacggcggca acctgacgct gcgcctggat gacgccgata tcgcgcttta
     8341 tcacgacaac ttccacccac aaccgcgcta tattccgctc agccaaccca tgcctttact
     8401 ggcaaatacg ccgtttattg tcaccggctc cggcaaattc ttccgtaacg tccagctcga
     8461 tcctgcagct aacttaggcg tcgtaaaagt cgacagcgat ggcgcgggct accatattct
     8521 ctgggggtta accaacgaag ccgttccaac ttctgaactt ccggctcact ttctctccca
     8581 ctgcgagcgc attaaagcca ccaacggtaa agatcgggtg atcatgcact gccacgccac
     8641 caacctgatc gccctcacct atgtactgga aaacgacacc gcggtcttta ctcgccaact
     8701 gtgggaaggc agcaccgaat gtctggtagt attcccggat ggcgttggca ttctgccgtg
     8761 gatggtgccc ggcacggacg aaatcggcca ggcgaccgca caagagatgc aaaaacattc
     8821 gctggtgttg tggcccttcc acggtgtgtt cggttccgga ccgacgctgg atgaagcctt
     8881 tggtttaatc gacaccgcag aaaaatcagc acaagtatta gtgaaggttt attcgatggg
     8941 cggcatgaaa cagaccatca gccgtgaaga gttgatagcg ctcggcaagc gcttcggcgt
     9001 tacaccacta gccagtgcgc tggcgctgta aggagcaaac atgatccgca aagcctttgt
     9061 catgcaggta aaccccgacg cccacgaaga gtatcagcgt cggcataatc ccatctggcc
     9121 ggaactggaa gcagtgttga aatctcacgg tgtgcataac tacgccattt acctcgacaa
     9181 agcgcgtaat ctgctgtttg ccatggtaga gattgaatct gaagaacggt ggaatgcggt
     9241 agccagtact gatgtttgcc aacgttggtg gaaatatatg accgatgtta tgcccgctaa
     9301 cccagataac agcccggtga gtagcgagct gcaagaagtg ttttacctgc cgtaattgcc
     9361 caatgtgccg gagatgcctc cggcacaata ttttaaattt cgatttgaaa aattaaacat
     9421 attacatctc ccggtaagtt atatttccct gatacattgt gagtaaatca caaaaataat
     9481 gaataaccca ttaatgattc atgtggttta tttaaataac cattatgtgc attactccgc
     9541 aaatctgacc tttcactctg tctcaaattg tcattgttta ccgacatatc gcctctcatc
     9601 acttaatcgc tccaccatta caattgatta aaatatattt tatggagtcc gattaatggc
     9661 agctcttact gcaagctgta ttgacctgaa tattcagggc aatggcgctt attccgttct
     9721 gaagcagttg gcgacaatag cgttacaaaa cggttttatc accgactcac accagttcct
     9781 gcaaaccctg ctgctgcgcg aaaaaatgca ctctactgga tttggttccg gtgtcgcagt
     9841 gccgcacggt aaaagcgcct gcgttaaaca accgttcgta ttattcgccc gcaaagcgca
     9901 ggctattgac tggaaagcca gcgatggcga agacgtcaat tgctggatct gcctcggcgt
     9961 gccgcaaagc ggcgaagagg atcaggtcaa aatcatcggc acactgtgtc gcaaaattat
    10021 tcaccaggaa tttattcatc aactgcaaca gggcgatacc gaccaggtgc tggccttgtt
    10081 aaatcaaacc ctcagctcat aaggaagtgg cgatggagtc atccttacgt attgtcgcga
    10141 tcaccaactg ccccgccggg atcgctcaca cctacatggt ggcggaagcc ctggaacaga
    10201 aagcccgttc tctcggccat accataaaag tggaaactca agggtccagt ggcgttgaaa
    10261 accgcttatc cagcgaagag attgccgctg ccgattacgt cattcttgct accgggcgtg
    10321 gcctgagcgg tgatgatcgc gcgcggtttg ccgggaagaa agtttatgag attgccatct
    10381 ccctggcgtt gaaaaatatc gaccagattt tcagcgaatt accgacaaac tcgcagcttt
    10441 ttgccgcaga tagcggcgtg aagcttggca aacaggaagt gcaaagcggc agcgtgatga
    10501 gccacctgat ggcgggcgtc tctgcggcgc tgccgtttgt catcggcggt ggtatcctgg
    10561 tggcgcttgc caacatgctg gtgcagttcg gtttgccgta taccgatatg tcgaaaggcg
    10621 cgccgtcgtt tacctgggta gttgaatcca tcggttacct tggcttcacc tttatgatcc
    10681 ccatcatggg cgcttatatt gcctcgtcga ttgccgataa accggctttc gctccggcgt
    10741 ttctggtttg ctatctggcg aacgacaaag cgctgctcgg cacccagtcg ggtgcgggat
    10801 tcctcggcgc ggtggtgctg gggctggcga ttggctattt cgtcttctgg ttccgcaaag
    10861 tgcgccttgg caaagcatta caaccgctgc tcggttcaat gctgatcccg ttcgttactc
    10921 tgctggtttt cggcgtgctc acttactacg ttatcgggcc ggtgatgtcc gacctcatgg
    10981 gcgggctgct ccacttcctg aataccattc ctccatcaat gaagtttgcg gcagcgtttc
    11041 tggtcggtgc gatgctggcg ttcgatatgg gcggccccat caataaaacc gcctggttct
    11101 tctgcttctc actgctggaa aaacacattt atgactggta cgccatcgtc ggcgttgtcg
    11161 cgttgatgcc gcccgttgcg gcgggtcttg cgacgttcat tgcgcccaaa ctgtttaccc
    11221 gacaggaaaa agaggctgcc agtagtgcca ttgtggtcgg tgccacagtc gcaaccgaac
    11281 cggctatacc ttacgctctg gcagctccgt tgccgatgat taccgccaat acgctggcgg
    11341 gcggcattac cggcgtgctg gtgatcgctt tcggaatcaa acgtctggca cctggtttgg
    11401 gtatctttga cccgctgatc ggcctgatgt cgccggtagg ttcgttttat ctggtgctgg
    11461 cgattggcct cgcgctgaac atttcattca ttatcgtcct gaaaggattg tggttacgcc
    11521 gcaaagcgaa agccgcgcaa caggagcttg tccatgaaca ttgagttact gcaacagttg
    11581 tgcgaagcca gcgccgttag cggcgatgaa caggaagttc gcgacatcct gataaacacg
    11641 ctggaacctt gcgttaatga aatcaccttt gatggtctgg gcagctttgt tgcccgtaag
    11701 ggaaataaag gtccaaaagt tgccgttgtc gggcatatgg atgaagtcgg ctttatggtc
    11761 acccacatcg acgagagcgg ttttctgcgt ttcaccacca ttggtggctg gtggaatcag
    11821 tcgatgctca accatcgggt aaccatacgc acacacaagg gagtgaaaat ccctggtgtg
    11881 attggtttcg tcgcgcctca tgcgttaacg gaaaagcaaa ggcaacagcc gctgtcattt
    11941 gatgagatgt tcattgatat tggcgcaaac agtcgcgatg aggtggaaaa acgcggtgtg
    12001 gcgatgggcg attttattag cccggaagcc aattttgcct gctggggcga agataaagtg
    12061 gtcggcaagg cgctggataa ccgcatcggc tgcgcaatga tggctgaact attgcagacg
    12121 gtgaataatc ccgaaattac gctgtatggc gtcggcagcg tggaagaaga agttgggcta
    12181 cgcggggcac aaacctcggc ggaacacatt aaaccggatg tagtgatcgt gctggatacc
    12241 gctgtcgcag gcgatgttcc gggcattgat aacattaaat acccgctgaa actgggccag
    12301 gggccggggc tgatgctgtt tgacaagcgc tacttcccca accagaaact ggtagcagcg
    12361 ttaaaaaact gtgccgcaca taacgattta ccgctgcaat tttccaccat gaaaaccgga
    12421 gcgacggatg gcgggcgcta caacgtgatg ggcggcgggc gtccggttgt tgcgctgtgc
    12481 ctgccaaccc gctatctgca cgctaacagc ggcatgattt caaaagccga ttacgatgct
    12541 ctgctcacgt tgatacgggg ttttctgacg accttaaccg cggagaaagt caacgcgttt
    12601 agccagttcc gtcaggtgga ttaatgctaa acgaacgcca gttaaagatt gtcgatctgc
    12661 tggagcaaca gccgcgcacg cctggtgagc tggcgcaaca gactggcgtt tcaggcagga
    12721 ccatcctgcg tgatattgac tatctcaact ttacccttaa cggcaaagcc cgcattttcg
    12781 ccagtggcag tgcgggctat cagctggaaa tcttcgagcg ccgcagcttt tttcagttgc
    12841 tgcaaaagca cgataacgac gatcggctgc tggcgctgtt attactgaat actttcactc
    12901 cccgtgcgca actcgcctcg gcgcttaatt tgccagaaac gtgggtagca gagcgtctgc
    12961 cccagttaaa acagcgttat gaacgcactt gttgcctggc cagccgccct ggtttgggcc
    13021 atttcattga tgagacagaa gagaaactcg ttatcttgct ggcgaacttg ctgcgcaaag
    13081 atccgttttt aattccgctg gcgggcataa cacgagacaa ccttcagcat ttatccacgg
    13141 cctgcgacaa ccaacaccgc tggccgctca tacagggtga ttatctctcc agcctgattc
    13201 tggcgattta cgccctgcgt aatcaactga ccgatgagtg gccgcaat
//
LOCUS       DAJTSI010000108        12993 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000108 DAJTSI010000000
VERSION     DAJTSI010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12993
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.108"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(128..607)
                     /gene="folK"
                     /locus_tag="OO327_002865"
     CDS             complement(128..607)
                     /gene="folK"
                     /locus_tag="OO327_002865"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR6019327.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="OO327_002866"
     CDS             complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="OO327_002866"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR6019328.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="OO327_002867"
     CDS             complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="OO327_002867"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR6019329.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="OO327_002868"
     CDS             complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="OO327_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR6019330.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="OO327_002869"
     CDS             complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="OO327_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR6019331.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="OO327_002870"
     CDS             complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="OO327_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR6019332.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4980..7409
                     /gene="hrpB"
                     /locus_tag="OO327_002871"
     CDS             4980..7409
                     /gene="hrpB"
                     /locus_tag="OO327_002871"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR6019333.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7605..10139
                     /gene="mrcB"
                     /locus_tag="OO327_002872"
     CDS             7605..10139
                     /gene="mrcB"
                     /locus_tag="OO327_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR6019334.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10359..12602
                     /gene="fhuA"
                     /locus_tag="OO327_002873"
     CDS             10359..12602
                     /gene="fhuA"
                     /locus_tag="OO327_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR6019335.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12653..>12993
                     /locus_tag="OO327_002874"
     CDS             12653..>12993
                     /locus_tag="OO327_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6019336.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPW"
BASE COUNT         3023 a         3307 c         3615 g         3048 t
ORIGIN      
        1 tgggattgat atttatctgc gggaatttat attatgtttc tgcaaaaatg tttaaactaa
       61 aatttttatt aaggaaagag aggatgtatg attttcagaa aatatttata ttcgtaatat
      121 aaataaatta ccatttgctt aatttgtcaa atgctcttgt atgtaagact tcacgcaacg
      181 tctccccatc aggaaacgcc agctccggtg caatctcaaa cagcggccac agcataaatc
      241 cacgattctt catatcgtag tgcggaacgg tcaggcgttc ggtatttatc acttcattac
      301 caaacagcat gatatcgaga tccagcgtgc gtggtcccca gcgttcagct ttacgaacac
      361 gaccctgttg cagttctatg cgctgtgtat ggttgagtaa ctcttccggg gcgggcgaag
      421 tttcaagcgc cacggcggcg ttgaggtaat cgggttgatc ttgcggcccc agcggtgggg
      481 tgcggtaaaa cgaagaaacg gcaagaatgc ggctttcagg gatatcgcct aatgctttca
      541 gggcagcatt gacctgctcc agcggagagg ccagattgct gcctatggca atatacgcca
      601 ctgtcatgtg gtaccctcac gacgcggtgc gcgtttgcgt ggacgacgag tacgacgacg
      661 cggtgacggt tcttcatcca gctcgttaag catccctttt tgatctggtg gtgcggaaac
      721 ctggaactca ccccaccatt tcaccagacg ctgcagttca gcattacgtt caacttcagc
      781 tcgcaaggcc aacaggtcat aagccgcacg gaacttagga tgctccagca gtttccatgc
      841 gcgtttaccc tgacgacggg acatacgcaa ctgcaactgc cagatatcgc gggttaacgt
      901 cgtcagacgt ttcgggattg ccagtgaacg gcaggcttcg tccagcacgt cgttcatcgc
      961 cagcgcgaaa gcgtcgtgat aggtcaggcc gctttcctgg gcgatcttct gtgccgtctc
     1021 cagcagtggg taccagaaca tggcggcaaa caagaacgcc gggttcacgc gcatatcgtt
     1081 atggatacgc gtatcggtat tcttcagcac ctgttcaatg atccgctcca tcgggctgtc
     1141 gccattttcc gtgaagtagc gggtaatggt cgggaacagc ggctggaaca gatgatattc
     1201 acacaacagc ttataggttt cgtaaccgta gcccgcttgt agcagtttaa gcgattcttc
     1261 aaacaggcgt gccggtggga tatcgttcag cagggtagcg aggcgaggga tcggttctgc
     1321 ggtttccggg ctgatgcgca tacccaattt ggcggcaaaa cgtaccgcgc gcagcatacg
     1381 taccggatct tcacggtagc gcgtttccgg gttaccaatc agacggataa cgccgtcctt
     1441 cagatccttc ataccaccaa cgtaatcacg gacggtaaaa tccgctacgc tgtaatacag
     1501 gctgttgata gtgaaatcgc ggcgctgggc gtcttcttcg atggagccga aaatgttgtc
     1561 gcgcagcaac atgccgtttt gcccgcgttg ggaggtcgtg cggtcgctga cgttaccttc
     1621 gtggtgtcca cggaaggtcg caacttcgat aatctccggg ccaaacatta catgagccag
     1681 acggaaacgg cgacccacca ggcggcagtt acggaacagt ttgcgcacct gctcaggcgt
     1741 ggcgttagtg gttacgtcaa aatctttcgg ctttttgcca agtaacaggt cgcgcacgcc
     1801 gccgccaacc agccaggctt cgtatcccgc tttgttgagc ctgtacatta ccttcagggc
     1861 attttcactg atatctttgc gggaaatagc atgctgctca cgcgggatca ccgtcacctg
     1921 tggacgggcg actgcctgtt cagcctcgct ttcctcgcgg cttagcacct tgcggcaaaa
     1981 attagcgact cgggtaaaaa tagtacacct cggtagtgtc aaacatcatt caggacaaaa
     2041 aaatagcggc taatcatagc tcagcatgac gcatttgaga atgttgaatt tacaattgcc
     2101 gactcgggca cggcggtcag catccagttt ttgacggctg actgaaggat ttgctcgacg
     2161 ctgaaatcct gccagtgtgc ttctgcctgc tgccccagaa attgaagtgc cgcgattagt
     2221 accgggcgtg gatcgccttt cggcaacgca ggcgcatgat tctgcttgga aagtttagcg
     2281 ccttgtggat taagcgccag cggcagatga atgtaatctg gcactttcca gccaaaaagc
     2341 tggtacagcg ggatttgcct tactgttggt tcaatcagat cagccccacg cactatttct
     2401 gtaacgccct ggaaatgatc atcaaccaca acagccaggt tgtaggcgaa caacccatca
     2461 cggcgatgaa tgataaaatc ttcccgtgcc agtttttcgt cggcgtgaat aatgcctcgc
     2521 agcaggtcag taaattgcgt gaccggatgc tgctggcgga tacgcactgc ggcgttgtct
     2581 ggtccatgat gcaacacccg gcaatgaccg tcgtaaatac cgccaatgct ttgaatacgc
     2641 gcacgcgtac aggtgcagta ataacttagt ccttgttcat gtaaccaggc gagtgcttca
     2701 cgataggcgt cgtgacgttg cgattgccag agaacatcgc cgtcccagtg cagaccgtaa
     2761 tgttccagct ggcgcaggat agtttctgcg gcaccgggaa cttcacgagg cgggtcgata
     2821 tcttctatgc gtaccagcca gcgaccttgc cgggcgcgag cctgcaaata gctgccgagc
     2881 gcggcgatca gagagccaaa atgaagctcg ccggaaggag agggggcgaa gcggccaata
     2941 tactgtgtgt ctgtcatctc tttgaacaaa aaataaggcg ggagcatttc ccgcctgtgg
     3001 taaacgtgat ggaacggctg taattagcca gccatctgtt tttcgcgaat ttcagccagc
     3061 gttttgcagt cgatgcacag atcggctgtc gggcgcgctt ccagacggcg aataccaatt
     3121 tcaacaccgc aggattcgca gtagccgaaa tcttcgtctt ccactttttt cagcgtcttc
     3181 tcgatctttt tgatcagctt acgctcgcga tcgcggttac gcagttcgag gctgaactct
     3241 tcttcctggg ctgcacggtc taccgggtcc gggaagttgg ctgcttcatc ctgcatatgt
     3301 gtaacggtgc gatcgacttc atccctgagt tgattacgcc atgcttccag aatacgacgg
     3361 aagtgcgcca gctgggcttc attcatatac tcttcgcccg gcttctcctg atatggttcc
     3421 accccagcga tggcgagaat actcagggac gatgttttac ggttttgccc ttcttgcatg
     3481 ttgcttctcc ttaacacgca ctatcgatcc ccatgttcgg gggaaaaatg aggccgctat
     3541 aaatagcaga tgcttttccg gatagcaatt atctaaacgt aacacttgac aactgtgtga
     3601 ggaaaagcgt atttgcgcac gcgaccagaa tgtaaattaa ccagttactt actttactac
     3661 aatgtaaccg gcagtgattt tttaagagcc atgccttcag cagaaatttc cgctttgtaa
     3721 gccagaattt ctacccccct ctgttgagct tctgacaata gttgcgcgta tttctcatcg
     3781 atgtggcgcg cgggtgaaaa ccgtgtaatg gctgaatgca gcacggcgaa aaaaataacc
     3841 gcacgctggc cttcagccgc tacgctcatc aactcccgaa ggtgtttctg acctcgttca
     3901 gtgaccgcat cgggaaaata tccctgttcg ttctccgcta acgtaaccga tttcacttca
     3961 atatagcagt ctggacgcga atccgcctgc aacataaagt caatacggct gcgttcggag
     4021 ccgtatttta cttcgctttt cagcgagcta tagcctgaca gttctgaaat tgattcatta
     4081 aggatagcct ctttcgtcaa cctgttagcc caaagcgtat tgacgcaaat aaatgcgccg
     4141 ctctggcttt gagttaattc ccaggtgtgt gggtatttcc gtttggtgtt gtctgaagtc
     4201 gaataccaga cggtatcgcc aggcgttgca caaccggtca tcgcacccgt attcgggcag
     4261 tgtagcgtta attcgcgacc atcgggtgtg atcacatcgg ctaaaaaacg tttgtaacgc
     4321 tgaattagcg tcgcgcgctg tagaggggga gaaaattcca ttgcgacaat ccttgttatt
     4381 gcgttagcgc ccagcgtttt agcggcgtgt agcgtgtgcg tccacgggca aacgaggagg
     4441 cgtaaagggt gaactccgtc accgcataag accagttaaa acccggcggc gggaatgtca
     4501 ccgcctcgct ggcgtcgcgc aataaggtaa tatgtggatg aaacggacga ttgctttgaa
     4561 aacaaccgct gcgggcagcc tgtgaacgga gcatattcgc cagctggatt aagccgcgtg
     4621 gaggctgacg catccctaac cacaccacac gcgaacgcag ccattgtccg gcgtcatcaa
     4681 gcgtgagtgt gaaaccaggt tgacgaatcc gtccggctaa aagagaaagc gccttctctt
     4741 tctctgcgct cacttcgcct aaaaatgcca gagtcaggtg caaattatcg gcggcgaccg
     4801 gacgtcccgc ctcaggtggg aagtgtgtgg cgcgccaatg gataatctgt tcgcggattt
     4861 ctgcaggtaa gtcgatagca aagaacagac gttgcggttc agacatgtga ggcactcggt
     4921 tatgaattac cgcgatgcta caatgtggcg cgaagaatgt taaccctctg gagcgttttg
     4981 tgtcgtcgtt gcccgttgct gccgtcttac ctgaattact taccgctctc gattgtgcgc
     5041 cgcaggtatt attaagtgcg ccgaccgggg ccgggaaatc aacctggctg ccgctgcaac
     5101 tgctggcgca tcccggcatt aacgggaaaa ttatcctgct ggagccgcgt cgtctggcgg
     5161 cgcgtaacgt cgcgcaacgg ctggcggagc tgcttaacga aaagccaggc gataccgttg
     5221 gctaccggat gcgtgcgcaa aactgcgtcg ggctgaatac ccgcctggaa gtggttaccg
     5281 aaggcgtgct gacgcggatg atccagcgtg acccggaact gagcggtgtt ggattggtga
     5341 tccttgatga gtttcatgag cgcagtttgc aggcggattt ggcgttggcg ctgttactcg
     5401 atgtgcaaca aggtctgcgt gatgacctta aactgctgat tatgtcggct acgctggaca
     5461 atgaccgctt gcagcaaatg ctgccggaag cgcccgtcgt catctcagaa gggcgctcgt
     5521 ttccggttga acgccgttat ttaccgctgc ccgcgcatca gcgttttgac gaagccgttg
     5581 cggtagccac cgccgaaatg gtgcgtcagg aaagtggatc attactgtta tttttacctg
     5641 gcgtcggaga aattcagcgc gtgcaggaac aactggcttc gcgcatcggc agtgatgtgg
     5701 tgctctgccc gctgtatggc gtgttgtcac tgaacgatca gcgaaaagcg atcctcccgg
     5761 caccgcaagg gatgcgcaaa gtggtgctgg cgaccaatat tgctgaaacc agtttaacca
     5821 tcgaaggtat tcgtctggtg gtggattgtg cccaggagcg tgtggcgcgc tttgatccgc
     5881 gcacggggct tacgcggttg attactcaac gcgttagtca ggcatccatg acgcagcgtg
     5941 ccgggcgcgc cgggcgtctg gagccgggta tcagcctgca tttaatcgcc aaagaacaag
     6001 cagaacgcgc cgcggcgcaa agtgagccgg agatcttaca aagcgatctt tccggtttgc
     6061 tgatggaatt actgcaatgg ggatgcagcg atccggcgca gatgagctgg ctggatcaac
     6121 cgccaacggt gaatctactg gccgcgaaac gcctgttaca aatgctgggg gcgctggagg
     6181 gtgaacggct tagtgcgcaa gggcaaaaaa tggcagcgct gggtaacgat ccgcgtttag
     6241 cggcaatgct ggttaacgca aagagcgatg acgaagctgc taccgcggca aaaattgccg
     6301 ccattctcga agagccgcca cggatgggca atagtgacct gggcgtggcg ttttcgcgca
     6361 atcaaccagc ctggcagcaa cgtagtcagc aactgttaaa acgcttaaac gtacgcggcg
     6421 gtgaggcaga cagttcgctt atcgcgccgc tacttgccgg ggcgtttgcc gatcgcattg
     6481 ctcatcgccg tgggcaagat ggacgctatc aactggcaaa cagcatggga gcgatgctcg
     6541 atgccgacga cgcgctaagc cgccacgaat ggttgatcgc accgttatta ttgcagggca
     6601 gcgcctcgcc ggatgcgcgg attttactgg cgctaccggt cgatattgat gaattagtac
     6661 aacgctgtcc gcagctggtg cagcaatctg acaccgtgga atgggatgac gcgcaaggta
     6721 cgctgaaagc ctggcgtcgg ctacaaatcg gtcagttgac ggtgaaagtg cagccgctgg
     6781 cgaaaccgtc agaagacgag ttgcatcagg cgatgcttaa tggcatccgt gataaaggtt
     6841 taagcgtgct caactggacg gcggaagcgg aacagctacg cttgcgtttg ttatgcgccg
     6901 caaagtggtt gccggaatat gactggccag cggttgatga tgaaagtttg ttggcgacgc
     6961 tggaaacgtg gctgctgcca catatgaccg gcgtacattc actacgcggc ctgaaatcac
     7021 tcgacattta tcaggcacta cgcggattac ttgattgggg aatgcagcaa cgtctggata
     7081 gtgaattgcc tgcgcattac actgtgccga cgggaagccg gatcgccatt cgttatcatg
     7141 aagataaccc gcccgcgctg gcggtgagaa tgcaggagat gtttggtgag gccactaatc
     7201 cgacgatcgc ccaggggcgc gtgccgctgg tgctgaagtt gctttcccct gcccaaaggc
     7261 cgctgcaaat cacgcgtgat ttgggggcct tctggaaagg ggcgtatcgt gaggtgcaaa
     7321 aggagatgaa agggcgttat cccaaacacg tctggccgga cgacccggca aacacggcac
     7381 cgacgcgacg gacgaaaaag tattcgtaag gttggttttc tccctctccc tgtgggagag
     7441 ggccggggtg agggcatcag cgcgcacgtt caccctcacc ctaaccctct ccctcaaggg
     7501 agaggggacc gatcgagcgc aaattttgag agatatcttc ttctgtcttg taacagaaga
     7561 acagaaaatc gggcttttgc gcctgaatat tgcggagaaa aagcatggcc gggaatgacc
     7621 gcgagccaat tggacgcaaa gggaaaccga cgcgtccggt caaacaaaag gtaagccgtc
     7681 gtcgttacga agatgacgat gattacgacg attatgatga ctatgaggat gaagaaccga
     7741 tgccgcgcaa aggtaagggc aaaggcaaag ggcgtaagcc tcgtggcaaa cgcggctggc
     7801 tatggctact gctaaaactg gctatcgttt ttgccgtgct gatcgccatt tacggcgttt
     7861 atctcgatca aaaaattcgt agccgtattg atggcaaggt ctggcaactg cccgcggcag
     7921 tttatggccg aatggtcaac cttgaaccag atatgaccat cagcaagaac gaaatggtga
     7981 agctgctgga ggcaacacag tatcgtcagg tgtcgaaaat gacccgtcct ggcgaattta
     8041 ccgtgcaggc caacagcatt gagatgatcc gccgtccgtt tgatttcccg gacagtaaag
     8101 aagggcaggt gcgtgcgcgt ctgacctttg atggtgatca tctggcgacc atcgtcaata
     8161 tggagaacaa ccgtcagttc ggtttcttcc gtcttgatcc gcgtctgatc accatgatct
     8221 cctcgccaaa cggtgagcag cgtttgtttg tgccgcgcag tggtttccct gatttgctgg
     8281 tggatacttt gctggcgaca gaagaccgtc atttttacga gcatgatgga atcagtctct
     8341 actccattgg gcgtgcggtg ctggcaaacc tgaccgcagg tcgcacggtg cagggtgcga
     8401 gtacgctgac gcaacagctg gtgaaaaacc tgttcctctc cagcgagcgt tcttactggc
     8461 gtaaagcgaa cgaagcttac atggcgctga tcatggacgc gcgttacagc aaagaccgta
     8521 ttcttgagct gtatatgaac gaggtgtatc tcggtcagag cggcgacaac gaaatccgcg
     8581 gcttcccgct ggcaagcttg tattactttg gtcgcccggt agaagagcta agcctcgacc
     8641 agcaggcgct gttagtcggt atggtgaaag gggcgtccat ctacaacccg tggcgtaacc
     8701 cgaaactggc gctggagcga cgtaatctgg tgctgcgtct gctgcaacag caacagatta
     8761 ttgatcaaga actctatgac atgttgagtg cccgtccgct gggggttcag ccgcgtggtg
     8821 gggtgatctc tcctcagcca gcctttatgc agctggtgcg tcaggagttg caggcaaaac
     8881 tgggcgataa ggtaaaagat ctctccggcg tgaagatctt cactaccttt gactcggtgg
     8941 cccaggacgc ggcagaaaaa gccgccgtgg aaggcattcc ggcactgaag aaacagcgta
     9001 agttgagcga tcttgaaact gcgattgtgg tcgtcgaccg ctttagtggt gaagttcgtg
     9061 cgatggtcgg aggttctgag ccgcagtttg cgggctacaa ccgtgcgatg caggcgcgtc
     9121 gttcgattgg ttcccttgca aaaccagcga cttatctgac ggctttaagc cagccgaaaa
     9181 tctatcgcct gaacacgtgg attgccgatg cgccaatcgc gctgcgtcag ccgaatggcc
     9241 aggtctggtc accgcagaac gatgaccgtc gttatagcga aagcggcaga gtgatgctgg
     9301 tggatgcgtt gacacgttcg atgaacgtgc cgacggtaaa tctggggatg gcgctggggc
     9361 tgcctgcggt tacggagacc tggatcaaac tgggcgtacc gaaagatcag ttgcatccgg
     9421 ttccggcaat gctgctgggg gcgttgaact taacgccaat cgaagtggcg caggcattcc
     9481 agaccatcgc cagcggtggt aaccgtgcac cgctttctgc actgcgttcg gtaatcgcgg
     9541 aagatggtaa ggtgctgtat cagagcttcc cgcaggcaga acgcgctgtt ccggcgcagg
     9601 cggcgtatct gacactatgg accatgcagc aggtggtaca acgtggtacg ggtcgtcagc
     9661 ttggggcgaa atacccgaac ctgcatctgg cagggaaaac agggactacc aacaataacg
     9721 tagatacctg gtttgcaggc attgacggca gcacggtgac catcacctgg gtaggccgtg
     9781 ataacaacca gccgaccaaa ctgtatggag ccagcggggc aatgtcgatt tatcagcgtt
     9841 atctggctaa ccagacgcca acgccgctga atcttgttcc gccagaagat attgcagata
     9901 tgggcgtgga ctacgacggc aactttgttt gcagcggtgg catgcgtgtc ttgccggtct
     9961 ggaccagcga tccgcaatcg ctgtgccagc agagcgagat gcagcagcag ccgtcaggca
    10021 atccgtttga tcagtcttct cagccgcagc aacagccgca acagcaacct gctcagcaag
    10081 agcagaaaga cagcgacggt gtagccggtt ggatcaagga tatgtttggt agtaattaac
    10141 atctaagcgt gaaataccgg atggcgagtt gccatccggt aaaataacat cccatctaag
    10201 atattaaccc tttcttttca tctggttgtt tattaaccct tcaggaacgc tcagattgcg
    10261 taccgcttgc gaacccgcca gcgtttcgaa tattatctta tctttataat aatcattctc
    10321 gtttacgtta ttattcactt tacatcagag atataccaat ggcgcgttcc aaaactgctc
    10381 agccaaaaca ctcactgcgt aaaatcgcag ttgtagtagc cacagcggtt agcggcatgt
    10441 ctgtttatgc acaggcagcg gttgaaccga aagaagacac tatcaccgtt accgctgcac
    10501 ctgcgccgca agaaagcgca tgggggcctg ctgcaactat tgcggcgcgg cagtcagcta
    10561 ccggcactaa aaccgatacg ccgattcaaa aagtgccaca gtctatttct gttgtgaccg
    10621 ccgaagagat ggcgctgcat cagccgaagt cggtaaaaga agcgcttagc tacacgccgg
    10681 gtgtctctgt tggtacgcgt ggcgcatcca acacctatga ccacctgatc attcgcggtt
    10741 ttgcggcaga aggccaaagc caaaataact atctgaatgg cctgaagttg cagggcaact
    10801 tctataacga tgcggtcatt gacccgtata tgctggaacg cgctgaaatt atgcgtggcc
    10861 cggtttccgt gctttacggt aaaagcagtc ctggcggtct gttgaatatg gtcagcaagc
    10921 gtccgaccac cgaaccgctg aaagaagttc agtttaaagc cggtactgac agcctgttcc
    10981 agactggttt tgactttagc gatgcgttgg atgatgacgg tgtttactct tatcgcctga
    11041 ccggtcttgc gcgttctgcc aatgcccagc agaaagggtc agaagagcag cgttatgcta
    11101 ttgcaccggc gttcacctgg cgtccggatg ataaaaccaa tttcaccttc ctttcttact
    11161 tccagaacga gccggaaacc ggttattacg gctggttgcc gaaagaggga accgttgagc
    11221 cgctgccgaa cggtaagcgt ctgccgacag actttaatga aggggcgaag aacaacacct
    11281 attctcgtaa tgagaagatg gtcggctaca gcttcgatca cgaatttaac gacaccttta
    11341 ctgtgcgtca gaacctgcgc tttgctgaaa acaaaacctc gcaaaacagc gtttatggtt
    11401 acggcgtctg ctccgatccg gcgaatgctt acagcaaaca gtgtgcggca ttagcgccag
    11461 cggataaagg ccattatctg gcacgtaaat acgtcgttga tgatgagaag ctgcaaaact
    11521 tctccgttga tacccagttg cagagcaagt ttgccactgg cgatatcgac cacaccctgc
    11581 tgaccggtgt cgactttatg cgtatgcgta atgacatcaa cgcctggttt ggttacgacg
    11641 actctgtgcc actgttcaat ctgtacaatc cggtgaatcc cgatttcgac ttcaatgcca
    11701 aagatccggc aaactccggc ccttaccgca ttctgaataa acagaaacaa acgggcgttt
    11761 atgttcagga tcaggcgcag tgggataaag tgctggtcac cctaggcggt cgttatgact
    11821 gggcagatca agaatctctt aaccgcgttg ctgggacgac cgataaacgt gatgacaaac
    11881 agtttacctg gcgtggtggt gttaactacc tgtttgataa tggtgtaaca ccttacttca
    11941 gctatagcga atcgtttgaa ccttcttcgc aagttgggaa ggatggtaat attttcgcac
    12001 cgtctaaagg taagcagtat gaagtcggcg tgaaatatgt accggaagat cgtccgattg
    12061 tagttactgg tgccgtgtat aatctcacta aaaccaacaa cctgatggcg gaccctgagg
    12121 gttccttctt ctcggttgaa ggtggcgaga tccgcgcacg tggcgtagaa atcgaagcga
    12181 aagcggcgct gtcggcgagt gttaacgtag tcggttctta tacttacacc gatgcggaat
    12241 acaccaccga tactacctat aaaggcaata cgcctgcaca ggtgccaaaa cacatggctt
    12301 cgctgtgggc tgactatacc ttctttgacg gtccgctttc aggtctgacg ctgggcaccg
    12361 gtggtcgtta tactggctcc agctatggtg atccggctaa ctcctttaaa gtgggaagtt
    12421 atacggtcgt ggatgcgtta gtgcgttatg atctggcgcg agtcggcatg gcgggctcca
    12481 acgtggcgct gcatgttaac aacctgttcg atcgtgaata cgtcgccagc tgctttaaca
    12541 cttatggctg cttctggggc gcagaacgtc aggtcgttgc aaccgcaacc tttcgtttct
    12601 aatttctctt ttggggcacg gatttccgtg cccatttcac aagttggctg ttatgcagga
    12661 atacacgaat cattccgata ccacttttgc actgcgtaat atctcctttc gtgtgcccgg
    12721 gcgcacgctt ttgcatccgc tgtcgttaac ctttcctgcc gggaaagtga ccggtctgat
    12781 tggtcacaac ggttctggta aatccactct gctcaaaatg cttggtcgtc atcagcctcc
    12841 gtcagaaggg gagattcttc ttgatgccca gccgctggaa agctggagca gcaaagcgtt
    12901 tgcccgcaaa gtggcttatt tgccgcagca gcttcctccg gcagaaggga tgaccgtgcg
    12961 tgaactggtg gcgattggtc gttacccgtg gca
//
LOCUS       DAJTSI010000109        12953 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000109 DAJTSI010000000
VERSION     DAJTSI010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12953
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.109"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..586)
                     /locus_tag="OO327_002875"
     CDS             complement(<1..586)
                     /locus_tag="OO327_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCR6019337.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGF"
     gene            complement(657..2771)
                     /gene="fusA"
                     /locus_tag="OO327_002876"
     CDS             complement(657..2771)
                     /gene="fusA"
                     /locus_tag="OO327_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR6019338.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2868..3338)
                     /gene="rpsG"
                     /locus_tag="OO327_002877"
     CDS             complement(2868..3338)
                     /gene="rpsG"
                     /locus_tag="OO327_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR6019339.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3435..3809)
                     /gene="rpsL"
                     /locus_tag="OO327_002878"
     CDS             complement(3435..3809)
                     /gene="rpsL"
                     /locus_tag="OO327_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR6019340.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3935..4222)
                     /gene="tusB"
                     /locus_tag="OO327_002879"
     CDS             complement(3935..4222)
                     /gene="tusB"
                     /locus_tag="OO327_002879"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR6019341.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(4230..4589)
                     /gene="tusC"
                     /locus_tag="OO327_002880"
     CDS             complement(4230..4589)
                     /gene="tusC"
                     /locus_tag="OO327_002880"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR6019342.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4589..4975)
                     /gene="tusD"
                     /locus_tag="OO327_002881"
     CDS             complement(4589..4975)
                     /gene="tusD"
                     /locus_tag="OO327_002881"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR6019343.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4975..5697)
                     /locus_tag="OO327_002882"
     CDS             complement(4975..5697)
                     /locus_tag="OO327_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6019344.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5864..6676)
                     /gene="fkpA"
                     /locus_tag="OO327_002883"
     CDS             complement(5864..6676)
                     /gene="fkpA"
                     /locus_tag="OO327_002883"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6019345.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKFSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6897..7115
                     /locus_tag="OO327_002884"
     CDS             6897..7115
                     /locus_tag="OO327_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR6019346.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVAAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7164..7754)
                     /gene="slyD"
                     /locus_tag="OO327_002885"
     CDS             complement(7164..7754)
                     /gene="slyD"
                     /locus_tag="OO327_002885"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6019347.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7849..8049)
                     /locus_tag="OO327_002886"
     CDS             complement(7849..8049)
                     /locus_tag="OO327_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR6019348.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(8059..9796)
                     /gene="kefB"
                     /locus_tag="OO327_002887"
                     /pseudo
     CDS             complement(8059..9796)
                     /gene="kefB"
                     /locus_tag="OO327_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9796..10347)
                     /gene="kefG"
                     /locus_tag="OO327_002888"
     CDS             complement(9796..10347)
                     /gene="kefG"
                     /locus_tag="OO327_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR6019349.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10478..12391
                     /locus_tag="OO327_002889"
     CDS             10478..12391
                     /locus_tag="OO327_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6019350.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12391..>12953
                     /locus_tag="OO327_002890"
     CDS             12391..>12953
                     /locus_tag="OO327_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6019351.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEAC"
BASE COUNT         3166 a         3540 c         3198 g         3049 t
ORIGIN      
        1 ggaagccagc cagttccagg attttcgctt cccactctgc gtcgccttcc agcgctttca
       61 gagcagaacc acgaacgatc ggagtgtcgt cgcccgggaa gtcgtactga gacagaagtt
      121 cacgaacttc catttcaacc agttccagca gctcttcgtc atcaaccatg tcgcatttgt
      181 tcaggaacac gatgatgtac ggaacgccta cctgacgacc cagcaggatg tgctcacgag
      241 tctgcggcat cgggccgtca gtcgcagcaa ctaccaggat cgcgccgtcc atctgagcag
      301 caccggtgat catgttttta acatagtcgg cgtgccccgg gcagtctacg tgtgcgtagt
      361 gacgggtcgg ggtgtcatat tcaacgtgag aagtgttgat ggtgatacca cgagcttttt
      421 cttccggcgc gttatcgatc tggtcgaatg cacgagcagc accgccgtag gttttagcca
      481 gtacggtggt gattgcagcg gtcagagtag ttttaccgtg gtcaacgtgg ccgatagtac
      541 caacgttaac gtgcggtttt gtacgttcaa atttttcttt agacacggct atattcctta
      601 ctatagtgct ctccccttca ggagagagca cgggactttg gtattaaccc ttaggcttat
      661 ttaccacggg cttcaattac ggcctgagca acgttactcg gcgcttcatc atacttcagg
      721 aattccatag tgtatgatgc acgacctttg gtcagagaac gcagctgagt tgcgtatccg
      781 aacatttcag acagcggtac ttcagcgtgg atcttaacgc cagtaacttc agattcctga
      841 cctttgagca taccacgacg acggctcaag tcaccgataa cgtcaccggt gttctcttcc
      901 ggagtttcta cttcaacctt catgatcggc tcaagcagaa ctggtttcgc tttcttaaag
      961 ccttctttaa aggcgataga agcagccagt ttaaacgcca gttcagagga gtcaacgtca
     1021 tggtaagaac cgaagtgcag acgaataccc atgtctacta ccgggtagcc tgccagcgga
     1081 cctgctttca gctgttcctg gataccttta tcaacggccg ggatgtattc gccagggatt
     1141 acaccacctt taatgtcgtt gatgaactcg tagcctttcg ggtttgaacc cggctccagc
     1201 gggtacatgt cgataacaac atgaccatac tgaccacgac caccagactg tttcgcgtgt
     1261 ttaccttcaa catcggtaac tttctggcgg atagtttcac ggtaagcaac ctgcggttta
     1321 cctacgttcg cttcaacgtt gaattcacgc ttcatacggt caacgatgat gtcgaggtgc
     1381 agttcgccca tacccgcgat gatggtctgg ttagattctt cgtcagtcca tacacggaaa
     1441 gacgggtctt ctttagccag acggcccaga gccagaccca ttttttcctg gtcagctttg
     1501 gttttcggtt caactgcgat ggagattacc ggctcaggga attccatacg ttccagaatg
     1561 atcggcgcat ccgggtcaca cagggtgtca ccagtggtta cgtctttcag gccgatagca
     1621 gcagcgatgt cgcccgcgcg aacttctttg atctcttcac gtttgttagc gtgcatctga
     1681 acgatacgac cgaaacgctc acgtgcagct ttcacggagt tcagtacggt atcaccagag
     1741 ttaaccacac cggagtaaac acggaagaag gtcaggttac caacaaacgg gtcggtagcg
     1801 attttgaacg ccagtgcaga gaacggctcg tcatcacttg cgtgacgttc agccggagtg
     1861 tctttaccgt cgtccaggat accgttgatc gcaggtacgt caaccgggga tggcaggtaa
     1921 tcaattaccg catccagcat cgcctgaaca cctttgttct tgaacgcaga accacaggtt
     1981 accaggatga tttcgttgtt cagaacgcgc tgacgcagag cacctttgat ttctgcttca
     2041 gtcagttctt caccacccag gtatttttcc atcagctctt cagaagcttc agctgcggat
     2101 tcgatcaggt tctggtgcca ttcgttagcc agttcaacca tgtctgccgg gatatcttcg
     2161 tattcgaagg ttacgccctg gtcagcgtca ttccagttga tagctttcat tttcaccagg
     2221 tcaacaacac cggtgaaatg ttcttcagca ccaatcgcca gctgcagcgg aaccgggttc
     2281 gcgcccagac gggttttgat ctggttaaca actttcagga agttcgcacc cacgcggtcc
     2341 attttgttaa cgaacgcaat gcgcggaact ttatatttgt ttgcctgacg ccatacggtt
     2401 tcagactgcg gctgaacacc accaactgcg cagtaaacca ttaccgcacc atcgagaaca
     2461 cgcatggaac gttctacttc gattgtgaag tcaacgtgcc ccggggtgtc gatgatgttg
     2521 atgcgatgcg gctcatactg cttagccata ccagaccaga atgcagtagt cgcagcggaa
     2581 gtgatggtaa taccacgttc ctgctcctgc tccatccagt ccatggttgc agcgccgtca
     2641 tgaacttcac cgattttatg gtttacaccg gtgtagaaca gaatacgttc ggtagtagtg
     2701 gttttaccgg cgtcgatgtg cgcactgata ccgatgttac ggtagcgtgc gatgggtgtt
     2761 gtacgagcca tttgtttcct cgtttatctt ttaggcgttc aatttaagta gcccaaagcg
     2821 ggctgcttac tggaagcgcc cgcctggtga ctaaaactcc gaagggatta ccaacggtag
     2881 tgtgcgaacg ccttgttggc ttcggccata cggtgaacgt cttcacgttt cttaactgca
     2941 gtacctttgt tttctgcagc atcagaaagt tcgttcgcca ggcgcagagc catggattta
     3001 tcaccgcgtt tacgagcagc ttcaacgatc caacgcattg ccagagcatt acgacgaacc
     3061 ggacggactt caactggtac ctgataagta gaaccaccaa cgcggcgaga cttaacttct
     3121 acagtcgggc gcacgttttc gagagctact tcgaatgctt ccagttcaga tttaccagag
     3181 cgctgagcca gggtctccag cgcgctgtat acgatagatt cagcagtaga ttttttacca
     3241 tctaccatca ggatatttac aaatttagcc agcagttctg atccgaactt cggatccggc
     3301 agaattttac gctgaccaat gacgcgacga cgtggcatgg aaatactccg ttgttaattc
     3361 aggattgtcc aaaactctac gagtttagtt tgacatttaa gttaaaacgt ttggccttac
     3421 ttaacggaga accattaagc cttaggacgc ttcacgccat acttggaacg agcctgctta
     3481 cggtctttaa cgccggagca gtcaagagca ccacgtacgg tgtggtaacg aacacccggg
     3541 aggtctttaa cacgaccgcc acggatcagg atcacggagt gctcctgcag gttgtgacct
     3601 tcaccaccga tgtaggaagt cacttcgaaa ccgttagtca gacgaacacg gcatacttta
     3661 cgcagcgcgg agttcggttt tttaggagtg gtagtatata cacgagtaca tacgccacgt
     3721 ttttgcgggc atgcttccag cgcaggcacg ttgcttttcg caactttgcg agcacgtggt
     3781 ttgcgtacca gctggttaac tgttgccatt aaatagctcc tggttttagc ttttgcttcg
     3841 taaacacgta ataaaacgtc ctcacacaat atgaggacgc cgaattttag ggcgatgccg
     3901 aaaaggtgtc aagaaatata caacgatccc gccatcacca ggccatctgg ctggagtgct
     3961 taaccgtaag tctgacgaaa tcagtatagt caatgggaat gatgtcgttc aaaatttgac
     4021 cagtcaaacc gcgggcaata aggtcttcgt tcagggcata gaccttaatg ggggcattac
     4081 gcagactttc aaggtagcgg ttaccgtcaa ctgcggcagt tacgccatct tgcaataata
     4141 gcagttcgtc tccttcactg agcagacgca gcagcgcagc aaaatccgtc agccagggtg
     4201 aacgatgtaa cgtgtgcagc ataaacgcct caaaacctca aaataacatc gtagttggcg
     4261 agttcgcggc gtaaggcatc tgcttcgagc ggcgtggctt cgacaacaaa gggtgtctgc
     4321 ggatctaacc cgcgttcgcg cagtgaagcc gcacaaaccc agcactgttc aatgtcgtac
     4381 agactcaaca atttaaaagt ggcaatgtaa tcacgcgcca gcactgcatc gggcttttgt
     4441 cctgacagca gctgaaaaac gccatcagca ataaagaaga cggccagatc gtcagttaat
     4501 gcggaagttg ccagtaaagc atctaaacct tcccgaccag cagctgtacc atgaggtgca
     4561 gtagaaaaaa caaacgcaat tcgtttcatc agaactgtac caccctgtca caggtcagcg
     4621 aggcttccgc cagcgcgcca agtccgctta aggtaaatcc cggctgaagg tttgacgaag
     4681 ccagccccag ccttcctgct tcagtttcat cgacgatacc gcggcgtaac gctgccgcta
     4741 cgcagatatt cagcgccaca ccatgttgcg cattcaactg ttgccagccg cgcacaaggt
     4801 caaattcgtc gcttgccggt gaggtcagtt ggttagcgtt atagacccct tcccgataga
     4861 aaaagacgct gcttaactta tggccttctg ctatcagcgc ctgcgcaaac tgaaaagcac
     4921 tactcgcctg ttgcgtaccg tatgctggcc cggtcaccac gatggcaaaa cgcattactt
     4981 atcttgcccc tgaaaatcac cgctcttgaa ctggcggatg tagagataga cagtgtgttt
     5041 ggagatgttc aggcggtcag caacctggtt gatcgcatct ttaatatcga agatcccttt
     5101 ctcgtagaga ttcagcacga tctgacgatt tttggcgtta ttagaaacat tgcgatcggc
     5161 attcacttct tcgatggtga actccagcgt ttgggtaacc agatcttcaa cagaagaggc
     5221 aaagttgacg cttgaaccga catccggggt ttctggcggc acaaaagtgc tcataatctg
     5281 cgagaaggga acatcaagat tcatattgat gcacagcaga ccaattacac gctgttcgcg
     5341 gttacgaatc gcgatagtca gggacttcat taatacgccg cttttggcgc gagtaaagta
     5401 gcatttagaa acgctgctat ccgctcccgt catatcgtgc agcatacgta gcgccaggtc
     5461 agtaattggc gaaccaatct tccggcctgt atgttcaccg ttagcaatgc gaatggctga
     5521 acattttaga tcctgcaaag agtgcaaaac gatttcacag tgggagccaa taagcatcgc
     5581 taacccgtcc accaccgctt cgtaggattt cagaatatca aaatcggtct ggtcgaaagg
     5641 acgttgatcc agtaaatcca actcactggt ttcgttggtt aaaagcgacc tggacatgaa
     5701 aaaaaacact ccttttcagg agcctgtcgt taacttttca gggcaggctc attaatgatg
     5761 cgggtaacta aattaataca gcggaggttc cgctttccag cactaattat atccggcctg
     5821 taataaaaaa accgccgcct ggtcaggcgg cggttcttaa tgcttatttt ttagcagaat
     5881 ctgcggcttt cgcatcagct tccggctttg catcagcctt cggcgctggt ttcacatcca
     5941 gcagctctac gtcaaacact agggtagaat tcggtgggat ccccggaaca cccgctttgc
     6001 cgtaagccag ttctggtgga ataaccagtt tgatcttacc gcctttcttg atgttcttca
     6061 gaccttctgt ccaacccggg ataacaccgt ccagacggaa agaaagcggt tcaccacggg
     6121 tgtaagagtt gtcgaactct ttaccgtcga tcagcgtacc tttgtagttc actacaacag
     6181 tatcgctgtc tttcggtgct tcgcctttac cggcttctac tacctgataa accagaccag
     6241 ttgaagaggt tttcacacct ttctctttgg caaatttctc gcggtactct ttaccttttg
     6301 cttcgttatc agccgcgtct ttttccatct tcgcctgagc agaagacttc acgcgagctt
     6361 cgaatgcttg cagagtctgt tcgatctctt ggtcggagaa tttgctctta tcagcaaatg
     6421 catcctgaac accagcgatc agctgatctt tatccagttt gatgcccagt ttttcttgtt
     6481 ctttaagaga gttttccatg taacgaccca gcgaagcacc cagtgcataa gctgatttct
     6541 gatcgtcatt tttgaacgat gctttgctgt ctgcagttgt agcaggtttt gcagcttcag
     6601 cagcaaaagt gattggtgca tgcagggcaa cggccattgt ggtcgccagt agcgttactt
     6661 taaacagtga tttcatccat atctccagga tcggggcatc tcaccccagg gttaactatt
     6721 atcagaaggg tactataaag cgttgtcgaa caaatctaca tacagacacg ccctattatc
     6781 atctattttc agactctttt tgtttaaatt agtttcgatg accgcgaaat gagtgctgtc
     6841 tcgggcagca aagttaagta gaatccgcgg cgaccattcg acaaaagagg tgaatcatgc
     6901 aggatttatc attggaagca cgcctggcag agctggagag ccgactggct tttcaggaaa
     6961 tcaccattga agaactgaac gtcacggtgg ccgctcatga aatggagatg gcgaaactgc
     7021 gcgatcatct gcgtctgctg accgagaagt taaaagccag ccagccgtcg aacatcgcgt
     7081 cgcaggctga agaaacgcca ccgccacatt attgaggcgt aaaaaaagcg gggattcccc
     7141 gcttttttgt caccttttcg gtattagtgg caaccacaac cgccgttgcc tttaccgcca
     7201 cagcagcctt cgccaccgtg ttcatgaccg tgatcgtggc catgaccgcc gcagcaaccg
     7261 tcgtggtcgt gatcgtggtg atgatcgtgc gcgccgtgaa cgtggccatg agccagttct
     7321 tcttcggtcg cttcgcgaat cgccacaact tcaacgttga atttcaggtt ctgaccggcc
     7381 agcatgtggt taccatcaac cactacgtga tcgtcttcaa ccgcagtgat ttcaaccggt
     7441 accggaccct ggtcagtttc agccaggaaa cgcataccta cctgcagttc atcaacgccc
     7501 ataaatacgt ctttaggaac acgttgcact aggttttcgt cgtactgacc gtaagcgtcg
     7561 ttcgcgccaa cagcgacatc aaatttgtcg ccaacttcat gaccttccag cgccgtttcc
     7621 aggccagaga tcagggaacc gtgaccatgc aggtagtcca gcggcgcact caccggagac
     7681 tcatcaacca acacaccgtc ttctgtacgt acctgatagg ccaggctgac caccaggtct
     7741 tttgctactt tcatgatatc tcctgagcat gggaagaata gtggcgcaga ttgtagcgga
     7801 attctgcagc cgtgtactca ctagcttaaa aaaactcggc gcatatcgct agtccggatg
     7861 aaaaatcccg atcacttgct catctttgtg aacgtgatcg cgggcttctt tgtctgcttc
     7921 tcgcatctga tgtccgcact taacacattc aacaatatca atattatttt cgcgccacat
     7981 cgccattgaa tcctgcgcct gacaggccgg gcattttgcg cccgctataa aacgttttcg
     8041 gattgccatc cttaccctct actcaaattc atcccagccg tccagctggc gtcgttcttg
     8101 ttgcatttca cgctggaaaa tctcttccag ttcgcgtcgg gcttccctgg cgcgagaaat
     8161 ttgtacggta tcagcatgca tcgggattaa ctcccgcagc attcgcatat ccaggcggcg
     8221 aaaatgcagt tgcgcccgct gtgcctgatg cggatgcatg ccaagcgtga ccagcgtctt
     8281 gcgccccagc tctaacgcac tggagaatgt ttcacgggaa aactgcgtca cccctgcctg
     8341 taataactca tgcgcttcca cacgtccgcg cgctcgcgca agaatatgca aatgcggaaa
     8401 gtgctgctgg catatttcca ccagcttcat ggtgtcttcc ggctcgttac aggtaatgac
     8461 gatagactca gcggcctctg cacccgcaga acgtaaaaga tcgacctgcg tggcgtcgcc
     8521 gtaataaact ttgtagccat atttacgcat caggttaacg gcgctgatat cccgctccag
     8581 cacggtaatg cgcattttat ttgccatcag caaacgacca atcacctgac caaaacgccc
     8641 gaagcccacg acaatgacct ggggtttatc atcgttgacc cacggttttt cgtcttcttc
     8701 ttccggtccg ttaaactggc gggatagcca tttatccacc agcttcatca gcaacggcgc
     8761 catctggtcg ccctggaata aacgttgtga agaagcggta gaaaagagga caaaggcaaa
     8821 ctcaccgccc tgactcaaca cgccagcaaa ctgcatccgc tctgagctac gcacgccata
     8881 caatcgcgcc agcagataca gcacgagaat tttcaccgcc accagcacaa ccacgcttat
     8941 cactacccac aacagatggg tataaagcac cccgaggttg agcgacatgc cgacagagat
     9001 aaagaacaaa ccgagcagca agcctttgaa gggatcgata gccgtttcca gttcatggcg
     9061 atattcactt tccgccagca gcacgcccgc aataaatgta ccgagcgcca tcgacagccc
     9121 cagcgcatcc ataaacaatg cggaacccaa caccagcagc agcgtcgcgg cggtgaacac
     9181 ttcccgcacg ccagaagctg caataaagcg gaataccgga cgcagtaaat agcgcccacc
     9241 aatcagcatg ccgacaaact ccagcacctt catgccgatc ttcatccagt cgaaatgttc
     9301 gtctgccgac cccgccaaca acggcactaa cgccagtgct gggatcactg ccagatcctg
     9361 aaacagcaga accgaaaacc cgagctggcc ggattcgctg cgattcatcc ctttctcacg
     9421 catcaattgc aacgccattg cagttgaaga catcgcaaag ccaatgccac cgaccaccgc
     9481 cgcctgccag gcgaaatccg tcagcatcaa taatcccgcc agcaacgccg cgcttaacag
     9541 cacctgtgcc gcgcctacgc caaaaatcga acgccgcagt tgccaaagtt tggagggatt
     9601 caactcaagg ccgatgataa acatcaggaa taccacgccg agttccgaaa agtggaggat
     9661 ctcatcgacg tcgctaataa accccagccc ccacgggcca attgcaatcc ccgccagcaa
     9721 atatcccaac acagcgccaa tccccagccg cgaagagaaa cagcactcct gcgagtaaaa
     9781 aatcggaacc ttccatcagc ggcctcctgg agacagcgga tttgccaacc agtcaccgta
     9841 ggctctggcg tggctcgcca gctcctttgc gctttgccgt ctcgcccagt aaataatgat
     9901 gggacttaac caatgcatcc ggcacatgcc cgccgccagt tcaaaggggc gcagcacatc
     9961 gctcatcggg tagcgattca gcgcgtcata acggtaagca ctttccggct cgccggtggt
    10021 aatcacgtta cgccagtact ttcccgccag ttggtttcct cccggcccgc tggcaaaacc
    10081 acgacttaat acccggtcca gccactcttt cagtagcgcc gggcagctat aggtataaag
    10141 aggatgctga aagacaatca cctcgtgctc gcgcagtaat gcctgctcac gggggatatc
    10201 aataaaaaaa tcgggatagt gcgcgtaaag gtcgtgcacg gtaacattgc tgagctgcgt
    10261 ggccggttta agcagtaccc ggtttgccac cgagtcctga gattccggat gggcatacag
    10321 cagcaaaact ttcgctggct gagacatcat ccccctcccg gtatggtttt tgtgtatagt
    10381 cgctgttttg ggctaccatt gcgcccggtg cggcagctcg cccatacatt acattatcat
    10441 aatgataagt taacatagtc tgaacatacg gcgccttatg attgttttct cctcgttaca
    10501 aattcgtcgc ggcgtgcgcg tcctgctgga taatgccacc gccaccatca accccgggca
    10561 gaaagtcggc ctggtgggta aaaacggctg tggtaaatct accctgctgg cattgctgaa
    10621 aaatgaaatc agcgccgacg gcggcagcta cacctttccg ggaagctggc aactggcgtg
    10681 ggtgaatcag gaaacgccag cgttaccgca agcggcgctg gaatatgtca ttgacggcga
    10741 ccgtgaatat cgtcaactgg aagcgcagct gcacgacgcc aacgaacgta acgacgggca
    10801 cgccattgcg accattcata gcaagctgga tgctattgac gcatggagta ttcgctcccg
    10861 tgccgccagc ctgctgcacg gcctcggttt cagcaatgaa caactggagc gcccggtaag
    10921 tgatttctcc ggtggctggc gtatgcgtct taaccttgcc caggcgctga tttgccgttc
    10981 agacttgctg ctgctcgacg aaccgactaa ccacctcgat ctcgatgccg ttatctggct
    11041 ggaaaaatgg ttgaagagct atcagggcac gctgatcctg atctctcacg accgcgactt
    11101 cctcgatccg atcgttgata aaattattca tatcgaacaa caaagcatgt tcgagtacac
    11161 cggcaactac agttcgtttg aagtacagcg cgccacccgt ctggcgcagc aacaagcgat
    11221 gtacgaaagc cagcaggaac gcgtggcaca tctgcaaagt tatatcgatc gtttccgtgc
    11281 caaagccacc aaagcgaagc aggcccagag ccgcattaag atgctggaac gtatggagct
    11341 gattgccccc gcacacgtcg acaacccgtt ccgctttagc ttccgcgcgc cggaaagcct
    11401 gccaaatccg ttactgaaga tggaaaaggt cagcgcaggc tatggcgatc gcattattct
    11461 cgactcgatt aaattgaatc tggtccccgg ctcgcgcatt ggtctgttag gccgcaacgg
    11521 cgcaggtaaa tcgacattaa tcaaactgtt agccggtgaa cttgcgccag tcagcggtga
    11581 aattggtctg gcgaaaggga tcaagctcgg ctacttcgcc cagcatcaac ttgaatacct
    11641 gcgcgccgac gaatcaccta ttcaacatct ggcacgttta gcgccgcagg agctggaaca
    11701 aaaactgcgt gactacctcg gcggctttgg tttcctgggc gataaagtaa ccgaagaaac
    11761 gcgccgcttc tccggtgggg aaaaagcccg cctggtgctg gcattaattg tctggcagcg
    11821 tccgaatctg ctgctgctcg acgaaccgac taaccacctt gacctcgaca tgcgtcaggc
    11881 actcaccgaa gcattaatcg agtttgaagg cgcgctggtt gtcgtttcgc acgaccgtca
    11941 tttgctgcgt tccaccactg acgatctcta cctggttcac gatcgtaaag tcgaaccgtt
    12001 cgacggcgat ctggaagatt atcaacagtg gttgagcgac gtacaaaagc aggaaaacca
    12061 gaccgacgaa gcgccaaaag agaacgcgaa cagcgcccag gcacgtaaag atcagaagcg
    12121 tcgggaagcg gagctgcgtg cgcaaaccca gccgctgcgt aaagagattg cccgtctgga
    12181 aaaagagatg gagaagctga acgcgcaact ggcgcaggcg gaagagaaac tcggcgacag
    12241 cgaactgtat gaccagagcc gtaaagcgga gttgaccgcc tgcctgcaac agcaagccag
    12301 cgccaaatcc ggcctggaag agtgcgaaat ggcgtggctg gaagcccagg agcagcttga
    12361 gcagatgctg ctggaaggcc aaagcaactg atggcgcaga taacgacgac cgatgccaat
    12421 gaatttagca gcagtgctga attcacccct atgcgcggct ttagcaattg tcatctacaa
    12481 actatgctgc cgcgtttgtt tcgtcgtcag gtgaaattca ccccgtactg gcagcggctg
    12541 gagttgcccg acggcgattt tgtcgatctc gcgtggagtg aagaccccgc acaggcgaaa
    12601 cataaaccgc gtttagtggt gtttcacggg ctggaaggca gtctcaatag cccttacgcc
    12661 cacggtctgg ttgaggcggc gcaaaagcgc ggctggctgg gcgtggtgat gcattttcgc
    12721 ggatgcagcg gtgaaccaaa ccgtatgcac cgcatttacc attcgggcga aaccgaagac
    12781 gccagttggt ttttacgctg gctgcaacgc gaatttggtc atgcgccaac ggctgccgtc
    12841 ggctattcgc tcggcggtaa tatgctggcc tgtttgctgg caaaggaagg caatgatctc
    12901 ccggttgatg cggcggtgat tgtctctgcg ccgtttatgc tggaagcctg tag
//
LOCUS       DAJTSI010000110        12906 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000110 DAJTSI010000000
VERSION     DAJTSI010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12906
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.110"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="OO327_002891"
     CDS             complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="OO327_002891"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR6019352.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1806..3224)
                     /locus_tag="OO327_002892"
     CDS             complement(1806..3224)
                     /locus_tag="OO327_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR6019353.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3378..3722)
                     /locus_tag="OO327_002893"
     CDS             complement(3378..3722)
                     /locus_tag="OO327_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCR6019354.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="OO327_002894"
     CDS             complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="OO327_002894"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR6019355.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4712..5647)
                     /locus_tag="OO327_002895"
     CDS             complement(4712..5647)
                     /locus_tag="OO327_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCR6019356.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5727..6842
                     /gene="yneK"
                     /locus_tag="OO327_002896"
     CDS             5727..6842
                     /gene="yneK"
                     /locus_tag="OO327_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR6019357.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6992..8182
                     /gene="ydeA"
                     /locus_tag="OO327_002897"
     CDS             6992..8182
                     /gene="ydeA"
                     /locus_tag="OO327_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR6019358.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8207..8872)
                     /gene="marC"
                     /locus_tag="OO327_002898"
     CDS             complement(8207..8872)
                     /gene="marC"
                     /locus_tag="OO327_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR6019359.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9084..9518
                     /gene="marR"
                     /locus_tag="OO327_002899"
     CDS             9084..9518
                     /gene="marR"
                     /locus_tag="OO327_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR6019360.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9538..9921
                     /gene="marA"
                     /locus_tag="OO327_002900"
     CDS             9538..9921
                     /gene="marA"
                     /locus_tag="OO327_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCR6019361.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9953..10171
                     /gene="marB"
                     /locus_tag="OO327_002901"
     CDS             9953..10171
                     /gene="marB"
                     /locus_tag="OO327_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR6019362.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="OO327_002902"
     CDS             complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="OO327_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCR6019363.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11365..12483
                     /gene="ydeE"
                     /locus_tag="OO327_002903"
     CDS             11365..12483
                     /gene="ydeE"
                     /locus_tag="OO327_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCR6019364.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            12610..12705
                     /gene="mgtS"
                     /locus_tag="OO327_002904"
     CDS             12610..12705
                     /gene="mgtS"
                     /locus_tag="OO327_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HCR6019365.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         3400 a         3299 c         2949 g         3258 t
ORIGIN      
        1 gtcaagctta caacattaac aggcgctgca tgtgacacct gttaatgata aaggaatata
       61 ggatgttgta actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac
      121 ggtgttatta gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca
      181 ggtcattagc aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt
      241 ctttttctgc caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct
      301 ggctccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt
      361 ctccgttacg ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg
      421 ccggaagtgt gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga
      481 acttggtcat accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga
      541 aacgcccggt gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg
      601 gaataatttc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
      661 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac
      721 cgttgaggat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
      781 tgagcggata tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa
      841 acaggtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
      901 gtttagccac ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
      961 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
     1021 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga
     1081 tgatccaacc tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg
     1141 tcagttttgc cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc
     1201 tgatacccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
     1261 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac
     1321 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac
     1381 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc
     1441 cagaattcag atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca
     1501 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
     1561 gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc cgtttgaatt
     1621 ggtgcgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga
     1681 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
     1741 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
     1801 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
     1861 ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc
     1921 gttcggtacg atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt
     1981 tgttgccagg tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga
     2041 ccatcgacag gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc
     2101 accagcccct tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta
     2161 tcgccacatt catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc
     2221 atgaccgtca gatgttgtgt ctgtttcagc gatggacttt tcagcgcctc ataaagaccg
     2281 gaccgtgagt aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt
     2341 agcaaattga tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca
     2401 ataccgagac gtgcggagaa catttccgga atggagaacg gcgaaccgac cgagatatca
     2461 atcaccgaat ttgccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat
     2521 gttacctgcg gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga
     2581 ctccccgctc cgccaatgac taccgaggca ataaccgaca caataaacgc gatggcgggc
     2641 atcatctgtt ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa
     2701 ggcacgatca acacgcccgt tgagaattgc tcactgaacc agtccgcaag caaaggccag
     2761 aaatccagac tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg
     2821 gcaatcagac agtaattaaa tagccgtaaa gcacttactg gctcatactt atttttcccc
     2881 agacgcttat cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa
     2941 ttgataacca gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc
     3001 acataactga tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc
     3061 gccattacgc cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca
     3121 ccaataaaat agaacagcgt ggttagcgca aaaatagcga tgctattacg tagcagatga
     3181 ccttcctgga acaaacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata
     3241 caattgcggg tggcaattat ctcaacgtga taagtaaatt ctcataaagg atagcatgtt
     3301 cgctaacagc ggcggcaaat aaaggtaccc tgggaatgct attttatcgt tttccgcaat
     3361 tctcatggct gattcatcta tacaaccagc cagtgataga ttaccgccac gatataacgt
     3421 tgttgttctt cgcttaacga aacaccctgc ggaatgttat gccacttagc cagacaaccg
     3481 cgacagcagg tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt
     3541 tgcttaccgt cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca
     3601 tgttgttcga tgactggcgc gcctttctcc aggcaatact gacgctcttt catgcccaga
     3661 cgaaagcgag agcgaaattt tgaacgggat aaacgcgcga agagtggatc gagagactgc
     3721 attaataaac cgaacgccct aactgtttcg tcaattgttc aaggacggcg atacccgcaa
     3781 gcgagttacc tgcatcatcc agttccggac tccagactgc gatggccatt tcatgcggaa
     3841 caatcgccac aataccgcca ccaacgccag atttcgccgg tagtcccacc cgccaggcaa
     3901 actcccccgc gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgcgcct
     3961 gcattggcgt caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga
     4021 caaacgtccg ggccagctct acacagctca ttttcagagc gcagtaatga aagtagtttt
     4081 gcagaacggt tgtcacgtca tgatggaaat tgccaaacga cttcatcagc caggcgatag
     4141 ccgcatttcg cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg tatgaaatat
     4201 cagacacacc gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttagtc
     4261 gcccttgcaa catatcgcag accaccagcg caccggtatt aatgaacgga ttacgcggaa
     4321 taccctgttc catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc
     4381 cgacgcgttg ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca
     4441 ctttggaaat agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc
     4501 cgtcaacggt acagatagca atccccaatc gggaaccgtc tactgtagcc agcgccggaa
     4561 tataatccgc gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta
     4621 aaattgcatt atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac
     4681 ctgcgagtat atcagagctg aatatgtcgc gtcagatccg gtctttccac actgtctgga
     4741 tattacagaa ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca
     4801 cgccaccaaa ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc
     4861 attccagacg tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg
     4921 aaaggccgaa ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg
     4981 tgattgcagc aacggggcca aacatttctt cccgaaacgc ggtcatttct ggggtaacat
     5041 tcgccagaac cgttggcgga tagtagttac ctgctccagc catcttttcc ccgcccagta
     5101 acaaacgcgc accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat
     5161 caaaacgagc cattggtccg agagcgttct cttcatcacg gggatcgccc attttcaagg
     5221 ctgccgcagc tgacacaaaa cgttcggtaa atgccgaagc aattccctct tcgataataa
     5281 agcgttttgc cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt
     5341 taaccgccag ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt
     5401 ccagtacgca ttttttcagt gccgctccag cacgaacact tccggtcacc gtgacagcag
     5461 caatgcgcga gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat
     5521 atacgccttg cgggattccc gcatctttaa acacttgggc aatgagctga gcacagccca
     5581 tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc
     5641 cacgcatcac ctgcctttga tagttcttgc agctcaaatg ccctatatag tgatgttccg
     5701 atgacttatg actatatggg gcaaatatgg ttacgccagt aagcatctgc aattacatat
     5761 ctcttcctga tgattttccc gctcgtaata ttgcacctca agtaaaagaa gttttaaagg
     5821 attttattga tgcactcagt accataatat gtgatgaaga gtggcgcacg tctttaaaca
     5881 ccaactcagc cacaaaaaag atatttaaca accttgataa cttatcatat attcagagga
     5941 cttcttttcg gggtaacgac acactataca atgaaaaggt tcagtttaaa ctcacttatc
     6001 ctgtcaagaa tggaagacac aaagaaaata ttgaatttca ggtagtaata aatttaagcc
     6061 ctatttattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
     6121 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
     6181 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
     6241 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
     6301 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
     6361 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
     6421 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
     6481 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
     6541 tagcagaaca agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat
     6601 tgcactatca ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag
     6661 catcattaag tgatgataaa attatcaaac atatggcgcg caggaccctc aaactgtttg
     6721 taaaaaatga aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct
     6781 cccctgtcag accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt
     6841 aaaccgtgat gttaccgact ctctgactcg gaacagaatc agcgtcagag aaacggaaaa
     6901 cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat
     6961 cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt
     7021 ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt
     7081 tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt
     7141 cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt
     7201 aatgaccagt caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc
     7261 cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg
     7321 tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc
     7381 tccggccggg aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat
     7441 ggtcttaggt ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt
     7501 cttcgcgatt ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt
     7561 actgcctagt gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc
     7621 attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag
     7681 ctatatcgag ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt
     7741 actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa
     7801 tcaatatgcg tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact
     7861 gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat
     7921 cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc
     7981 taccgacgtc gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc
     8041 gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc
     8101 ggtgcctgct tttgctgcgt taatatggtc aatcattata tttcgccgct ggccagtgac
     8161 actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt
     8221 taatgatttc caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc
     8281 ccatcaggcg ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg
     8341 agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa
     8401 ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa
     8461 tcatcgctat ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat
     8521 tggcactggg ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg
     8581 ctttctgttg cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa
     8641 ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg
     8701 ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg
     8761 cactgttcat gttgcctgcc aggccaagaa atagcgcgac agttgttaat gggttagcta
     8821 acggcagcaa caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt
     8881 gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt
     8941 cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg
     9001 gcatcggtca attcattcat ttgacttata cttgcctggg caatattacc ccctacaact
     9061 aattacttgc caaggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc
     9121 cattgggtcg cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc
     9181 tgtctccgct ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg
     9241 cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc
     9301 gtatgctgga tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac ccgaatgata
     9361 agcgcggcgt actggtaaaa cttaccacca gcggcgcggc aatatgcgaa caatgccatc
     9421 aattagttgg ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagttg
     9481 caacacttga gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg
     9541 tccagacgca atactgacgc tattaccatt catagcattt tggactggat cgaggacaac
     9601 ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatggcac
     9661 ctgcaacgga tgtttaaaaa agaaaccggt cattcattag gccaatacat ccgcagccgt
     9721 aagatgacgg aaatcgcgca aaagctgaag gaaagtaacg agccgatact ctatctggca
     9781 gaacgatatg gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa ttactttgat
     9841 gttccgccgc ataaataccg gatgaccaat atgcagggcg aatcgcgctt tttacatcca
     9901 ttaaatcatt acaacagcta gttgataacg tgacaacgtc actgaggcaa tcatgaaacc
     9961 actttcatcc gcaatagcag ctgcgcttat tctcttttcc gcgcagagcg ttgcggaaca
    10021 aaccacgcag ccagttgtta cttcttgtgc caatgtcgtg gttgttcccc catcgcagga
    10081 acaaccaccg tttgatttaa atcacatggg tactggcagt gataagtcgg atgcgctcgg
    10141 cgtgccctat tataatcaac acgctatgta gtttgttctg gccccgacat ctcggggctt
    10201 attaacttcc cacctttacc gctttacgcc accgcaagcc aaatacattg atatacagcc
    10261 cggtcataat gagcaccgca cctaaaaatt gcagacccgt taagcgttca tccaacaata
    10321 gtgccgcact tgccagtccg actacgggca ccagtaacga taacggtgca acccgccagg
    10381 tttcatagcg tcccagtaac gtcccccaga tcccataacc aacaattgtc gccacaaacg
    10441 ccagatacat cagcgacaag atggtggtca tatcaatagt aaccagactg tgaatcatgg
    10501 atgcggaacc atcgagaatc agcgagacaa caaagaaggg aatgattggg attaaagcgc
    10561 tccagattac cagcgacatc accgccggac gcgttgagtg cgacatgatc tttttattga
    10621 agatgttgcc acacgcccaa ctaaatgctg cagccagggt caacataaag ccgagcatcg
    10681 ccacatgctg accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg
    10741 cgatccccgc caattgtttg ccatgcagtc gctccccgaa agtaaacgcg ccaagcacga
    10801 tagtaaaaaa cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc ataccaaagt
    10861 taatggcgca gaacagaaag gcaaactgcg caaaactgat ggttaatcca taccccaaga
    10921 gcaaattcag tggtactttc ggtcgtgcaa caaaaaagat ggccggaaaa gcgaccagca
    10981 taaagcgcaa accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca
    11041 caaaatttag cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca
    11101 ttctaccgcc tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct
    11161 ttaacagttg attcgttagt cgccggttac gacggcatta atgcgcaaat aagtcgctat
    11221 acttcggatt tttgccatgc gtccgatggt taataacaac gacgaggcaa gaagggcgct
    11281 ggtagagcgt cgtagggata agttcatttg tctgtccggc agtgcgtttc gttatgtttt
    11341 attttagaga tgtaaagaaa tagcatggct acgctgccat ttatgaccat ttacttgagt
    11401 cgccagtaca gcctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt
    11461 ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat
    11521 atgttactgg caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac
    11581 gtgacgctgg ttgtgctctt ttttgccctc attaactgcg cctattctgt ttttgctacc
    11641 gtgctgaaag cctggtttgc cgacaatctt tcgtccacca gcaaaacgaa aatcttctca
    11701 atcaactaca ccatgctaaa cattggctgg accatcggtc cgccgctcgg cacgctgttg
    11761 gtgatgcaga gcatcaatct gcccttttgg ctggcagcta tctgttccgc gtttcccatg
    11821 cttttcattc aaatatgggt aaagcgcagc gagaaaatca tcgccacgga aacaggcagt
    11881 gtctggtcgc cgaaagtttt attacaagat aaagcactgt tgtggtttac ctgctctggt
    11941 tttctggctt cttttgtaag cggcgcattt gcttcatgca tttcacaata tgtgatggtg
    12001 attgctgatg gggattttgc cgaaaaggtg gtcgcggttg ttcttccggt gaatgctgcc
    12061 atggtggtta cgttgcaata ttccgtgggc cgccgactta acccagctaa catccgcgcg
    12121 ctgatgacag caggcaccct ctgtttcgtc atcggactgg tcggttttat tttttccggc
    12181 aacaacctgc tattgtgggg tatgtcagct gcggtattta ctgtcggtga aatcatttat
    12241 gcgccgggcg agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat
    12301 ttttccgccc agtctttagg ctggcttggt gccgcgatta acccattagt gagtggcgta
    12361 gtgctaacca gcctgccgcc ttcctcgctg tttgtcatct tagcgttggt gatcattgct
    12421 gcgtgggtgc tgatgttaaa agggattcgt gcaagaccgt gggggcagcc cgcgctttgt
    12481 tgatttaagt cgaacacaat aaaaatttaa ttcagccttc gtttaggtta tctctgctaa
    12541 tatctttctc attgagatga aaattaaggt aagcgaggaa acacaccaca ccataaacgg
    12601 aggcaaataa tgctgggtaa tatgaatgtt tttatggccg tactgggaat aattttattt
    12661 tctggttttc tggccgcgta tttcagccac aaatgggatg actaatgaac ggagataatc
    12721 cctcacctaa ccggcccctt gttacagttg tgtacaaggg gcctgatttt tatgacggcg
    12781 aaaaaaaccg ccagtaaacc ggcggtgaat gcttgcatgg atagatttgt gttttgcttt
    12841 tacgctaaca ggcattttcc tgcactgata acggatcgtt gacacagtag catcagtttt
    12901 ctcaat
//
LOCUS       DAJTSI010000111        12662 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000111 DAJTSI010000000
VERSION     DAJTSI010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12662
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.111"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            128..1825
                     /gene="maeA"
                     /locus_tag="OO327_002905"
     CDS             128..1825
                     /gene="maeA"
                     /locus_tag="OO327_002905"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6019366.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1959..2969
                     /gene="adhP"
                     /locus_tag="OO327_002906"
     CDS             1959..2969
                     /gene="adhP"
                     /locus_tag="OO327_002906"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCR6019367.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3115..3399
                     /locus_tag="OO327_002907"
     CDS             3115..3399
                     /locus_tag="OO327_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6019368.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="OO327_002908"
     CDS             complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="OO327_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR6019369.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="OO327_002909"
     CDS             complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="OO327_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR6019370.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="OO327_002910"
     CDS             complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="OO327_002910"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7630..7632),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6019371.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8449..9330
                     /gene="yddG"
                     /locus_tag="OO327_002911"
     CDS             8449..9330
                     /gene="yddG"
                     /locus_tag="OO327_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR6019372.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9585..10685
                     /gene="ompC"
                     /locus_tag="OO327_002912"
     CDS             9585..10685
                     /gene="ompC"
                     /locus_tag="OO327_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6019373.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNKRTDNSHLYGADDTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            10849..11334
                     /locus_tag="OO327_002913"
     CDS             10849..11334
                     /locus_tag="OO327_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR6019374.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYINHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            11444..12433
                     /locus_tag="OO327_002914"
     CDS             11444..12433
                     /locus_tag="OO327_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR6019375.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNN"
BASE COUNT         3341 a         3127 c         2941 g         3253 t
ORIGIN      
        1 acgattaaac accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac
       61 gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt
      121 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      181 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      241 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      301 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
      361 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
      421 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
      481 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
      541 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
      601 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
      661 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
      721 aacgaacaac caacagctgc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
      781 cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
      841 gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
      901 ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac
      961 agtcggcaca ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat
     1021 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
     1081 ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt
     1141 tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
     1201 gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt
     1261 gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga
     1321 agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa
     1381 cccgacgtct cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc
     1441 gctggtcgcc actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat
     1501 cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg
     1561 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc
     1621 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt
     1681 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa
     1741 aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
     1801 cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt
     1861 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
     1921 cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga
     1981 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
     2041 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
     2101 ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg
     2161 gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag
     2221 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
     2281 atgccggata cagcgttgat ggcgggatgg aggaagagtg catcgtggtc gccgattacg
     2341 cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg
     2401 tcaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct
     2461 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca
     2521 aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atggatgcag
     2581 atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg
     2641 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
     2701 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
     2761 atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc
     2821 aggatctaac cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc
     2881 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
     2941 gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgactccc atgttcgggt
     3001 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
     3061 aacaataatg atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa
     3121 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
     3181 agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg
     3241 accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt
     3301 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg
     3361 gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ctataaagcg
     3421 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
     3481 cgttcacttc aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc
     3541 atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     3601 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3661 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3721 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3781 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     3841 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     3901 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     3961 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     4021 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4081 gcgacgaaaa tcgcaatgcc gaagaagggg tgcaaaatgc gtcccatctg cggcgtaccg
     4141 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4201 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4261 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4321 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4381 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4441 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     4501 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4561 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4621 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4681 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4741 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4801 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     4861 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     4921 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
     4981 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     5041 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     5101 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     5161 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5221 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5281 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5341 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5401 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5461 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5521 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5581 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5641 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5701 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5761 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     5821 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     5881 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     5941 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     6001 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     6061 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     6121 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6181 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6241 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6301 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6361 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6421 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6481 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6541 cgggttaaag ccctggcaga aataaccggt aactttgcct tcatccatca tgttgaaata
     6601 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6661 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6721 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6781 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     6841 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     6901 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
     6961 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7021 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     7081 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     7141 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7201 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7261 ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc
     7321 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaatg ccagacagga acgtaatgtc
     7381 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7441 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7501 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7561 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7621 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7681 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7741 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7801 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     7861 gtcagaacct ggcgcacgat atttcgggta gcgcagacgg ttttcactgt tgacgtaatc
     7921 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     7981 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     8041 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     8101 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     8161 tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8221 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8281 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8341 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8401 ataaaattca gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa
     8461 aaagcaacgc tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt
     8521 cgcggtgtca gcgaggggct cggcccggtc ggcggcgcag ctgctatcta ttcattaagc
     8581 gggctgctgt taatcttcac ggttggattt ccgcgtattc ggcagattcc gaaaggctat
     8641 ttactcgccg ggagtctgtt attcgtcagc tatgaaatct atctggcgct ttccttaggg
     8701 tatgcggcga cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc
     8761 ctgacaattc tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca
     8821 ggattattat tagccctggt cggcgtctgc tgggtgttag gcggtgagaa tgggttacat
     8881 tatgatgaaa tcatcaataa tatcaccacc agcccattga gttatttcct ggcgtttatt
     8941 ggtgcgttta tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac
     9001 ggaattaccg tttttgtcct gctaacggga gcatgtctgt ggatttacta ttttctcacg
     9061 ccacaaccag aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt
     9121 accttaggat ttgcttatac tgcatggaat gtcggtatat tgcatggcaa tgtcaccata
     9181 atggcggtag gctcgtattt tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc
     9241 agcgccccgc tgtcgttctc gttctggcaa ggcgcactaa tggtctgcgg cggttccctg
     9301 ctctgctggc tggcgacacg tcgtggttaa aataattgcc gggtctacgg cccggcagtt
     9361 aatcagataa attccgacaa tattaaaaaa taaatttcaa ataccagtaa ttcaccttat
     9421 atctcatttc gacttcgatc acatatcgat aaataatatt attctatttt tctcattgtt
     9481 tttaatcacc attctatttt aaaaacagta tgtcatttat aaatgacgcc gtttaaaatt
     9541 cgtttagaaa atacgtttaa cgataattaa aaggattctg ttaaatgaaa ttaaaaatag
     9601 ttgcggtggt tgtaactggt ttgttagctg cgaacgtagc acacgctgcc gaagtctata
     9661 acaaggatgg taataaactc gacctttatg gcaaggttac cgctctacgt tattttactg
     9721 atgataagcg tgacgatggt gataaaactt atgcccgtct cggctttaaa ggagaaacgc
     9781 aaatcaatga tcaaatgatt ggttttggtc actgggaata tgattttaaa ggctataacg
     9841 atgaagccaa cggctcgcgc ggcaacaaga cccgtctggc ctatgctggt ttaaaaatta
     9901 gtgaatttgg ctctctggac tatggtcgta actacggtgt cggctacgac attggttcat
     9961 ggaccgatat gttgccagaa tttggtggcg atacctggag tcagaaagat gtcttcatga
    10021 cataccgtac caccggtcta gcaacctatc gcaactacga tttctttggc ttaattgaag
    10081 ggctgaactt tgccgcgcaa tatcaaggca aaaataaacg tactgacaac agtcatcttt
    10141 atggtgctga cgacacgcgt gccaacggtg acggtttcgg tatctcctca acttatgttt
    10201 atgatggctt tggtatcggt gcggtatata ccaaatccga tcggacaaat gcgcaggaaa
    10261 gagccgcagc taatcctctc aatgcctccg gtaagaatgc agaactatgg gccacaggta
    10321 taaagtatga tgccaacaac atctactttg cagctaatta cgatgaaaca ttaaacatga
    10381 ccacctatgg ggatggttat atctctaaca aagcacaaag ttttgaagtg gtggcgcaat
    10441 atcaattcga cttcggcttg cgcccatcac tcgcttacct gaaatcgaaa ggcagagatc
    10501 tgggccgcta cgccgaccag gacatgattg agtatatcga cgttggtgcg acgtatttct
    10561 tcaacaaaaa tatgtcgacc tatgttgatt ataaaatcaa cctgattgat gaaagcgact
    10621 ttacccgtgc cgtagatatt cgcaccgata acatcgtcgc tacgggcatt acctatcagt
    10681 tctaacgtcc tctcctgacc agcccttctc tgggctggtt tttgatttaa gtgctgctgt
    10741 atgactcctt aagtaaaaat gagcaattct tgatatttct ggtcattcac ctcaattaat
    10801 attattccat atcctccggg gacatcttac tataatattt attgatgagt gattatgacc
    10861 aatattaata cagcttgtgt aaaaaataat gccagttatc aacttaataa cgcattacct
    10921 aacaaagaaa ccatctccag caatttttgt gaacgactag aacaatgggg taataaatcg
    10981 gttaataatg gtgaagaaag agcaattgcc gtggagcgaa ttaaagaagc ttacaattcg
    11041 aacatggcat ctcttgattt gtcatatctt gatttgagtg aattaccgcc tatcccctcg
    11101 acagttaata cgttaaatct ggaaaacaat tgtctaactt gtcttgactt agctgacaat
    11161 gccagtctcg ttaatatcaa cctcagcgtt aataaaatta acacgataac ctttccaaaa
    11221 gaaccaaaac tggaaaacat ttatattaat cataacaatc tggaaagttt agatttaaaa
    11281 aatcagcatt cattggttaa tctggaagcg caaaataata gcctaaaaaa atgatatttc
    11341 tgatagttat aaactgaaat ttcttaatct tgattataac aagctagcat cgctggatgt
    11401 ctcccggcaa gaatctctga ttgagttaag tgcccatcac aatatgatca ctgaccttat
    11461 attacacaat catcccagga tgaaaacaat cactttaaac gacaaccata ttgcacattt
    11521 aaacgccaaa aacactacaa aactggaata tttaaactta agcaataaca atttactgcc
    11581 aaccaatgac attgatcaac taatatcatc aaaacatctt tggcatgtat tagttaacgg
    11641 catcaacaat gatccacttg cacaaatgca gtactggact gcagtaagaa atataattga
    11701 tgacactaat gaagtgacca ttgatttatc aggacttaat ttaaccactc aaccaccagg
    11761 gctgcaaaac ttcacctcta tcaatcttga taataaccaa ctcacacatt ttgatgcaac
    11821 caactatgat agactcgtaa agctaagtct gaatagtaat gctcttgagt caataaattt
    11881 tcctcaaggc agaaatgtaa gtattacaca tatatctatg aataataatg ctctcagaaa
    11941 tattgatata gataggcttt catcagttac ttattttagt gcggcacata atcaactaga
    12001 gtttgtgcaa ttagaatctt gcgaatggct gcaatacctg aatctcagcc ataatcaatt
    12061 aactgatatt gttgcaggta ataaaaatga actcttactg ctggatctat cccataataa
    12121 actaacaagt ttacacaatg tcttatttcc caacttgaat acgttactta ttaacaacaa
    12181 tttgctttct gaaattaaaa tattctatag caacttccgc aatgttcaga cattaaacgc
    12241 tgctaacaac cagttgaaat atataaatct tgatttcctg acttatcttc catctatcaa
    12301 aagtttaaga ctggacaata ataaaataac ccacattgat actaataata catccgatat
    12361 tggaacttta ttccccataa taaaacagag caaaaactta aattttttaa atgtttctgg
    12421 gaagaacaat tgacctacta tgcagctcat gttatttaat ttgttttccc cagcacttaa
    12481 gcttaatact ggcctggcaa ttctttcgcc tggtgcattt gaagttcact ctgacggaat
    12541 agatgcggat aacgaattgt ttcactatac tattaaaaaa gcatataccc catataatat
    12601 acacacttac aagacagagg aagttgtaaa ccagaggaat ataaaagtta aaaacatgac
    12661 ct
//
LOCUS       DAJTSI010000112        12635 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000112 DAJTSI010000000
VERSION     DAJTSI010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12635
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.112"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            758..841
                     /locus_tag="OO327_002915"
     CDS             758..841
                     /locus_tag="OO327_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR6019376.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(895..2247)
                     /gene="gorA"
                     /locus_tag="OO327_002916"
     CDS             complement(895..2247)
                     /gene="gorA"
                     /locus_tag="OO327_002916"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR6019377.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="OO327_002917"
     CDS             complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="OO327_002917"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR6019378.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3364..5406
                     /gene="prlC"
                     /locus_tag="OO327_002918"
     CDS             3364..5406
                     /gene="prlC"
                     /locus_tag="OO327_002918"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR6019379.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5414..6166
                     /gene="rsmJ"
                     /locus_tag="OO327_002919"
     CDS             5414..6166
                     /gene="rsmJ"
                     /locus_tag="OO327_002919"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR6019380.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6215..7684)
                     /gene="dtpB"
                     /locus_tag="OO327_002920"
                     /pseudo
     CDS             complement(6215..7684)
                     /gene="dtpB"
                     /locus_tag="OO327_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(8002..8436)
                     /gene="uspA"
                     /locus_tag="OO327_002921"
     CDS             complement(8002..8436)
                     /gene="uspA"
                     /locus_tag="OO327_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR6019381.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8830..9165
                     /gene="uspB"
                     /locus_tag="OO327_002922"
     CDS             8830..9165
                     /gene="uspB"
                     /locus_tag="OO327_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR6019382.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9236..10735)
                     /gene="pitA"
                     /locus_tag="OO327_002923"
     CDS             complement(9236..10735)
                     /gene="pitA"
                     /locus_tag="OO327_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR6019383.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            10967..12166
                     /locus_tag="OO327_002924"
     CDS             10967..12166
                     /locus_tag="OO327_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR6019384.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3109 a         3169 c         3356 g         3001 t
ORIGIN      
        1 taacagaaat gacatattgc gctcctgatt gttgcaggta gtgtctctct tcgaagcgga
       61 taagtcaaaa acatatatga cttaacgaat gtgtaagtgc agaggaaggt aataggtgtg
      121 aattttgagt tggctattca tttgaaaggc ggtttgaatc aggacgtaaa aaaaccgact
      181 ttgcgtcggt tttttactct tcagcctgag ttggtggctc tggctggagt gagaatgccc
      241 acatctaacg gttcaatact attagccagt ggctaacatt gcaagcgtta gcgatttctt
      301 cagctggaga agggacagtt tgctatacat ggtggtgaaa gaggatttat atactttcat
      361 cgggtcgctg ccgtaccaag ccttgtccga atccggacgt aaaaaaaccg acttcgcgtc
      421 ggttttttta ctcttcagcc cagagttggt ggctctggct ggagtgagaa tgccatcatc
      481 taacgcatca atgctaatac catactgaaa ctattgcaag gttatgctgg ttatttagcc
      541 taattacact gtatgattat ccagttagct ctgaggcatt ttcactctgg caatgcgcat
      601 aaacgctttc aaagtcctgg tcagaagtac gggtggtgcc gttaactgat gctctggccg
      661 gagtgagatt gcccacatct aacaatgagg catgcgccgt gacaggcagt ggatgagtaa
      721 gcggatgcat tctcactcca tcgcatggag aaaacgggtg attgataaag caatcatcgt
      781 tcttggggcg ttaattgcgc tgctggaact gatccgcttt ctgcttcagc ttctgaactg
      841 atagcggaaa cgtaattaag ggctaagagc acactactct tagcccttta acatttaacg
      901 catggtcacg aactcttctg ccgccgttgg gtgaatggcg acggtattgt cgaagtcttt
      961 tttggttgcg cccatcttca gcgccaccgc gaagccctgc aacatttcgt ccataccaaa
     1021 gccaatgccg tgaataccga caatcttctc ttctggccca acgcacacca gcttcatgcg
     1081 gcacggctgg cggtgagtgg tgacggcggt atacatcgcg gtgaaagagg atttatacac
     1141 tttcacctga tcgtcgccgt actgctcgcg cgcctgcggt tccgttaaac caacagtacc
     1201 aatcggcgga tggctgaaga ccacggtcgg aatgttgctg taatccagat gctcatccgg
     1261 cttgttatta aacaggcgtt cagagagacg gcgacccgct gcaactgcca ccggtgtcag
     1321 ttccactgca cccgtgttat cgcccaccgc gtaaatacct tcaatattgg tgttttgata
     1381 tttatcgacg acgatatagc ctttttcgtt ggttttaacg ccagcggctt cgaggttgat
     1441 gttgtcattg gcaggctcgc gaccaatcgc ccaaatcagg caatccaccg tttcactgcg
     1501 accatcttcc agctccagcg tcaggctacc atcggcattt ttcactaccg ctttcgggat
     1561 ggcgttggta tgcaactgtg ggccttcggc gttcatcact tcgaccagcg tttcagagat
     1621 catcgggtcg aagctgcgca gcggcgcatg tttacgcaca aacagatgcg ttttcgcgcc
     1681 gaggccgtta atcacgccag ccagctcaac ggcgatgtaa cccgcgccaa caaccgccac
     1741 gcgctctggc aaagcaggaa gggcgaagaa gccatcggaa tcaataccgt attccacgcc
     1801 tggaatatcc gggtggctcg gacgaccgcc ggtggcgatc agaatatgat cggccgtgat
     1861 ggtttcgccg ttaacttcca gcgttttggc atcaacgaag cgggcaaagc ctttgattac
     1921 atcaacgtta tttttaccga gcacgttttc ataggaagta tgaatacggt cgatataggc
     1981 ggtacggctg gcgatcaacg tttcccagtt gaacttgttg atggtggtat cgaaaccata
     2041 atcagggccg tacatatgga tcgcttcacg gatttgcgcc gcgtgccaca tcactttttt
     2101 cggcacgcag ccgacgttca cgcaggtgcc gcccagctct ttggcttcaa tcagtgcgca
     2161 tttctggccg tacatagccg cgcggttgat ggaggcgata ccgccgctgc cgccgccgat
     2221 ggcgatgtaa tcatagtgtt tagtcatgac atattgtcct tatcgttgat taccgcgatt
     2281 gtagcgcgag acgtaatagg tgccagcaat ggctgcaatt actccggcac gatccagctt
     2341 acggtggcgt gcccggtacc tgccggaacc agtttgctgt gcagccacga cagcacgtta
     2401 ttcatctgct gttccagctt ccacggcggg ttaatcacaa tcatgccgga agcggtcatg
     2461 ccacggcgat cgctgtctgg cagcaccgcc agttcaattt gcagaatttt gcgaatgccg
     2521 gtcgcttcca ggtcgtggat catgcgctta atttgctggc gcagcaccac cggataccac
     2581 aatgcgtaag tgccagtggc gaaacgtttg taaccttctg ctatcccgct gaccaccgcc
     2641 tgatagtcgg ttttcatttc atacggcggg tcgataagga ttaaaccacg gcgagaaacc
     2701 ggcggcagtt tggccttaag ttgctggaaa ccgtcggctt tttcgacgcg tgcacggctg
     2761 tctttctgaa atccagaacg cagcaacggg taatcgctcg ggtgcagttc ggtcagttgc
     2821 aggctgtctt gttcgcgcag tagctggcga gcaatcaacg gcgaaccggg gtagtaacgc
     2881 aactggccgc tacggttgaa gtgttttacc acattgatgt acgcctccag ttctgcgggc
     2941 aaatcgtcct gctgccagat acgggcgata ccttcgagat attcgccggt acgctcggca
     3001 tgttcgctac ctaactgata acgcccggcc cctgcgtggg tgtcgagata gagaaacggt
     3061 ttatctttct ctttcagcga ctcgatgatc aggctctgaa cggtatgttt aaggacgtcg
     3121 gcgtggttgc cagcgtgaaa gctgtggcga taactgagca tgggtaaagg tgttccggta
     3181 agtaaaaatt ggcccgtatt cggggcgcac aaaagcctat caggacagta taccgaaaag
     3241 aggccgccgc cgcgaaagcg taacgtttct cattgaaatt cactacactt aaccccatgc
     3301 tacacacatt atgtaaagcg cctgttgagc gcttccttaa cctctttaac caggactgcg
     3361 ctaatgacga atccgttact gactcccttt gaattgcctc cgttttctaa aattctcccg
     3421 gaacatgtcg ttccagccgt gactaaggca ttgaacgact gccgcgaaaa tgtggagcgc
     3481 gtagtagcgc aaggggcacc gtacacctgg gaaaatctct gccagccgtt ggcggaagtg
     3541 gacgatgttt tggggcgtat cttctccccg gtcagccacc tgaactcggt gaaaaatagc
     3601 ccggaactgc gtgaagcgta cgaacaaacc ctgccgctgc tgtcggaata cagcacctgg
     3661 gtagggcaac atgaagggct gtataaagcg taccgcgacc tgcgcgatgg cgatcattac
     3721 gccacgctga acacggcgca gaagaaagcg gttgataacg ccctgcgtga ttttgaactc
     3781 tctggcatag gtctgccgaa agagaaacag cagcgttacg gcgaaattgc tacccgtctt
     3841 tctgaactgg gcaaccagta cagcaacaac gtcctcgatg cgacgatggg ctggaccaaa
     3901 ctcgttaccg acgaagcggg gctggcgggg atgccagaaa gcgcgctggc tgcggcaaaa
     3961 gcccaggccg aagcgaaaga gctggaaggc tacctgctga cgctggatat cccaagctac
     4021 ctgccggtaa tgacctactg cgacaaccag gctctgcgcg aagagatgta tcgcgcttac
     4081 agcacccgcg cctccgatca aggcccgaat gccggtaagt gggataacag caaggtgatg
     4141 gaagagatcc tcgcgctgcg tcacgaactg gcgcaactgc tgggctttga aaactacgcc
     4201 tttaaatccc ttgccactaa aatggcagaa aacccgcagc aggtgctgga tttcttaacc
     4261 gatctggcaa aacgcgcgcg tccgcaaggc gaaaaagagc tggagcaact gcgtgccttc
     4321 gctaaagccg aatttggtgt cgatgagttg cagccgtggg atatcgctta ctacagcgaa
     4381 aaacagaaac agcacctcta cagcatcagc gacgagcagc tgcgtccgta cttcccggaa
     4441 aacaaagcgg ttaacggcct gtttgaagtg gtgaaacgta tttacggcat caccgctaaa
     4501 gagcgtaaag atgttgatgt ctggcatccg gatgtacgtt tcttcgaact gtatgacgaa
     4561 aataacgaac tgcgcggcag cttctacctc gatctgtatg cccgtgaaaa caagcgcggc
     4621 ggggcgtgga tggatggctg cgtaggccag atgcgtaaag ctgatggttc tctgcaaaaa
     4681 ccggtcgcgt atttgacttg taacttcaac cgcccggtaa atggtaaacc ggcgctgttc
     4741 actcacgacg aagtgatcac cctgttccac gagttcggtc acggcctgca ccatatgctg
     4801 acccgcatcg aaaccgctgg tgtttccggt atcagcggtg tgccgtggga tgcggtcgaa
     4861 ctgccgagtc agtttatgga aaactggtgc tgggagccgg aggcgctggc gtttatctct
     4921 ggtcactatg aaaccggcga accgctgccg aaagagttgc tggataaaat gctggcggcg
     4981 aagaactacc aggcggcgct gtttattctg cgtcagctgg agttcggcct gtttgatttc
     5041 cgccttcatg ccgagttccg cccggatcag ggggcaaaaa tcctcgaaac tctggcagaa
     5101 atcaagaaac tggttgccgt ggtgccatct ccgtcctggg gccgtttccc gcacgctttc
     5161 agccatattt tcgccggtgg ttatgcggca ggttactaca gctacctgtg ggctgacgta
     5221 ctggcggcag atgctttctc gcgctttgag gaagagggca ttttcaaccg tgaaaccggg
     5281 cagtcgttcc tcgacaacat tctgagccgt ggcggttcag aagagccgat ggatctgttc
     5341 aaacgcttcc gtggtcgtga accgcagctg gatgcgatgc tggagcatta cggcattaag
     5401 ggctgatcat tcagtgaaaa tctgcttaat tgatgaaaca ggcgccggag acggcgcctt
     5461 atctgttctg gcggcccgct gggggctgga gcacgatgaa gacaacctga tggcgctggt
     5521 gttaacgccg gaacatctgg aattgcgcaa gcgtgatgag ccaaaacttg gcggcatctt
     5581 tgttgatttt gttggcggag cgatggcgca ccgacgcaaa ttcggcggtg gtcgcggtga
     5641 ggcggtggcg aaagcggtgg gcattaaagg cgattatttg ccggatgtgg tggacgccac
     5701 cgcagggctg gggcgcgatg cctttgtact ggcttcagtc ggctgccgcg tgcggatgct
     5761 ggagcgtaat ccagtggttg ccgcgctact cgacgacggc ctggcgcgtg gttatgcgga
     5821 tgcggaaatc ggcggctggt tgcaggagcg gttgcagtta attcacgcct ccagcctgac
     5881 ggcgctaacc gatattaccc cacgcccgca ggtggtctat ctcgatccga tgttcccgca
     5941 taagcagaaa agcgcgctgg tgaaaaaaga gatgcgtgtc tttcagtcgc tggtggggcc
     6001 ggatcttgat gccgatggat tactggagcc tgcacgcttg ctggcgacca aacgcgtggt
     6061 ggtaaagcgc ccggactacg cgccaccgct ggcgaatgtc gccacgccaa acgcggttgt
     6121 gactaaaggg catcgctttg atatctatgc aggtacgcca gtgtaaaagt aaagcagggt
     6181 agcgataacg ctaccctgct ttctgcaaga ataattaatg gctttccggc gtcgcaatca
     6241 tgcgtttcag ccacggcacc atcaacagca tcactactgc aacgcccagc gtgaccagac
     6301 caatcttacc aaacacgttg gtatagacgg gcaacgtctc aagcggatcg gtaatgttgt
     6361 ccggcaccgc ggtaaatgtt gccacatagc cgcccagcaa gaacgcggca gcctgcgtca
     6421 ggaaccacat cccgagaata aagcccatca aatgctgcgg caccagggca gcaatcatcg
     6481 ccaggccaag ggcgctaata aacaattcac ccaggctcta gaataaatac accagcacga
     6541 taaaccatgg cgatgtcagc ccctggtcat ccgcaaacca cattcccgca gcagcagccg
     6601 tcaaaaagcc cagtgagcac ataaacatgc tgagagtaaa tttcatcggc atcgagaggt
     6661 ctttgccttt gttacccaga tgcgtgtaaa tgcctgccag tattgggctg gcgagtacca
     6721 cccagaacgg gttaagcgcc tggaagctga ccgggttgat ggaaaaaccg agaatttcat
     6781 gatgcacgtt gttgatggca aagaagttca gcgatgttgg catctgggcg tagagaatgt
     6841 aaaacaccac cgcttcgagc atcaggacaa aggcgacaaa cattttattg cgcccggttt
     6901 tatccagctt gaatgcctga cgaaagaaga tgatagtgac gacgatggag agaacaatca
     6961 gcaccagatt ggcgacttct acgttgtgca tcagccatgc acatacgaag atcatcacca
     7021 cgctgccaag taatacgtac aacagtttgc tgaagctcat cggcttgaag tcgggttcag
     7081 aaccaatgtc tttcaccatt ccacgacagg cgatgtaaac cagtaatgcg ataattaacc
     7141 ccgccccgca caggttgtag gtgactgaat aaccgaatct atcagcgatc acaggggcca
     7201 gcgataacgc tatcaacgag ccgatattga tcgacatata gaacagggtg aatgcgccat
     7261 caagccgcgg atctttcggc ggatagcact tcgaaagcaa gctggctggg ttagctttaa
     7321 acaggccgtt accgacagcg atagtcccca gggcgatgaa aatcaggtca ggcttaagta
     7381 gcgacatgcc ggtcatgaag tagccaatcg ccagcacaag tgctccaaga acaatggtgc
     7441 gtttggtccc caacaggtgg tcgccgacat agccgccaat ggaaatgagg ccatagacca
     7501 gtgcagcaaa agcaccaaaa gtgacaaaag cctgctcttg cgagaatcca agctgtttaa
     7561 cgaagaaaac cgccagtacg ccctgcacgc cgtagtagcc gaatcgctcc cataactcga
     7621 caaaaaagat catgaaaaat gggcgaggtt gctgcagcat ccccatgggt gttgttgtat
     7681 taatatttat taaccttcca ataccatcct gaaaagtatg aacgcgtaag ggataacgcg
     7741 cgaaaaagtt tacatctgtc agagattctt aatattgatg cggtgaatag attaatacac
     7801 tattacaaca gaaaataacc agctgatcac actaatgatg gaaaatatga tggtgttaaa
     7861 ttttgtattg gcaggagatt ttgattgcgt acgcagatcg taggccagat aaggggttta
     7921 cgctgcatca ggtaacaagg cgcgccaaaa agcaaaacgc ccgcatatgc gggcgttcag
     7981 gaacacgtcg tggagggatg attattcttc ttcgtcgcgt agcggaacaa tcagcatatc
     8041 aacgtgaacg gtgttgatca gctgacgtgc ggaagacatc agtttgctcc agaagtcctg
     8101 gtggtgacca caaaccacca gatccatatc gtatttcttg attgcatcga ccagaacctg
     8161 gcctaggtcg ccgctgccgc tcagggtttc agtgattgga tagcctgcat tagtggaaag
     8221 ctcggtcagc gcatgatgtg tctcttcaga gatgcgtttc tgcatatcac ccagattcac
     8281 atcaataagc ccggtgtata ggtcagagta gtttacatct acgtggatca gagaaacttt
     8341 cgcattgtag gggcgagcca tagagactgc tttctctacc agaactttgc tttccgggga
     8401 gagatcgact gcgatgagaa tgtgtttata agccatagtg ttactccttc cataaagttg
     8461 tcgatgactg gccagctagc gtttcttgtg ccgttacgtc acccgcgtcc tgcgaactac
     8521 gtgcgcgggg ctaacccagc cgtaaagatt cagtgttaag accatcctta ccttatagcg
     8581 acccggatga tccgtcaatc cgccttgctt acccgttaga taaacaaatt ttcgacatga
     8641 atattatatt gatagtggtt aaccttctgg aaaaaaaaca acctgatctc ctacactatc
     8701 tatagagccg ctcggatgtt ggtcaccaca aaagcggttt ctccaggacc tggtgttaac
     8761 tgattggcag cgtatcggga gcggtagccc cggggtggaa ctccgcgggc aggtcgccgg
     8821 ggaggaggta tgataagcac cgtcgcatta ttttgggctt tatgtgtcgt ttgcattgtt
     8881 aacatggcgc gctatttctc atcactacgc gcgttgttag tggtactgcg taactgcgat
     8941 ccattgctct atcaatatgt tgatggaggg ggctttttta cctcacatgg ccaacccaac
     9001 aaacaggtgc gtctcgtttg gtatatctat gcccaacgtt atcgcgatca tcacgatgat
     9061 gagtttattc gccgctgtga gcgggtgcgt cggcagttta ttctgactag cgcattgtgt
     9121 ggtctggtgg tggtcagcct gattgcattg atgatttggc attaagcact tcagcaataa
     9181 taacgtataa aaaaagcggg ccagtaaact gacccgctcg ttttggtgcg tacgattaca
     9241 ggaactgcaa ggagaaccag tacagcccgc cggaaagtag taccgcagcc ggaagggtaa
     9301 acacccaggc catcagaata ctggttacgg ttttacgctg taagccgccg ccatctacca
     9361 ccatcgtccc cgcgacagaa gaggagagta cgtgagtggt ggaaaccggc atcccggtat
     9421 aactcgccag gccgattgac actgccgccg tcatctgggc agacatcccc tgagcgtagg
     9481 tcatgccttt cttaccgatt ttctcaccga tagtcgttgc cacacgacgc cagccaatca
     9541 tcgtaccgat acctaacgcc agcgcgaccg ccatgatgat ccacaccggc gcatactcga
     9601 tggtgctaag catgtcggac ttcagtttct tcaacaggcg ctgatcgtca gcactcacac
     9661 caggcatctt caccactttg tcgatagtgt cagaaacgca cagcataatg cggcgcatct
     9721 ggctacgttg atcaagcgac agcttgtcgt agctttccac atcggtggtc aacatacctt
     9781 tcaggcggtt gagcgcgtta atggtattcg acggatggca gtggaactcc gcaggttgcg
     9841 ttgcgccagc ttccggagcc ggtactaact gatcagcacc ggtagcctgt ttgagcagcg
     9901 caggatgctg ctcaaagtaa gcttcgacgt tgttgatggc atcacgggta cgggtgattt
     9961 cgtagccagt ggcattcatg ttcaccacga agcctgctgg cgcgacgcca atcaatacca
    10021 acataaccag accaatgcct ttctgaccat cgttcgcgcc gtgcgaaaac gccacgccga
    10081 tagcggaaag gatcagcgcg atacgcgtcc agaacggcgg ctttttcttg ccgtctttct
    10141 tttcacgctc cgctggggtc aggtggatac gggcgcgttt cttggtgccg ctccagtagc
    10201 gacgcagcaa gaaaatcaga ccgccagcaa acaccaggcc gacaataggg gaaacgatca
    10261 gagaaccgaa aatacttaat actttcggga tattgagtgc atccaccact gacgtcccgg
    10321 tcatcaacgc attggttaaa ccaatcccga tgatcgcgcc aatcagcgta tgagagctgg
    10381 atgcaggtaa accaaagtac caggtaccca ggttccagat aatcgccgcc agcaacatag
    10441 agaacaccat ggcaaggcca tgagacgatc ccatattaag cagcagatcc gtcggcagca
    10501 tatgcacaat ggcataggca acactcagac cacccagcaa aacacccaaa aagttgaaca
    10561 ccgccgccat aaccacggcg agctgagaac gcatcgcgcg ggtatagata acggttgcca
    10621 cggcgttggc tgtgtcatgg aaaccattga tggcttcgta gaacagcaca aaagccagtg
    10681 caagcaataa taacagcccg gtatgcaaat ccaggccagc aaacaaatgt agcataggac
    10741 gttacgccat tttgaggaca tgaacgcggc gcattatcag tgactttcgc ggcgcgggca
    10801 aagtgaaata tagacttttt ttgatttgcc tcctgtatgg atttcactca aaaaataatt
    10861 atcttatata attcaggcaa atacttcctt ttagtaatat tgatgctggt gcgaccactg
    10921 aggaatcttt acaattcacg cccgtttttt ctaagaggag cgcaacgtgg aaaggtttga
    10981 tgccattatt ataggcgctg gtgcggcggg tatgttctgt tctgcgctgg caggtcaggt
    11041 aggacgccgg gttctgctga tcgataatgg taaaatacta gggcgcaaaa tccttatgtc
    11101 tggcggtggg cgctgcaact ttaccaacct ttatgtcgaa ccaggcgctt atctgagcca
    11161 gaatccgcat ttttgtaagt ctgcactcgc acgttttacc cagtgggatt tcattgatct
    11221 ggtcaataaa cacggtatcg cctggcacga gaaaacgtta gggcaactct tctgcgatga
    11281 ctccgcgcag cagattgtcg acatgctggt ggatgagtgc gagaagggca atgtaacctt
    11341 cagattgcgt agcgaagtgc tgagtgtggc gaaggatgaa acaggcttca cgcttgatct
    11401 gaacggcatg actgtcggtt gcgaaaagct ggtcatcgcg accggtgggc tgtcaatgcc
    11461 ggggctgggc gcatcgccgt ttggttataa gattgccgaa caatttggcc tcaacgtgct
    11521 gccgacccgc gcgggtctgg tgccattcac tctgcataaa ccgttgctcg aagagttaca
    11581 ggtgctggcg ggcgtggcgg tgccttccgt gattaccgct gaaaacggca ccgttttccg
    11641 tgagaactta ctcttcaccc accgcggctt atctggaccg gcggtgttgc agatttcaag
    11701 ctactggcaa ccgggtgaat ttgtcagtat caatctgcta ccggatgtgg acctcgaaac
    11761 cttcctgaat gagcagcgta acgcacatcc gaatcaaagc ctgaaaaaca cactggcggt
    11821 tcatctaccg aagcggttgg ttgaacgctt acagcaactc gggcaaatcc cggatgtttc
    11881 gctaaaacag ctcaacgtgt gtgaccaaca ggcactgatt agcacattga ccgactggcg
    11941 cgtacaaccc aacggcactg aaggctatcg cactgccgaa gtgacgctcg gcggcgtgga
    12001 caccaacgaa ctttcttcac ggacgatgga agcgcgcaaa gtgcctgggc tgtacttcat
    12061 cggtgaagtg atggacgtta ccggctggct ggggggctat aacttccagt gggcgtggtc
    12121 gagtgcatgg gcttgtgcgc aggatttggc gtcaattgtg aaataaccat gacgttttcc
    12181 cgtcagccgt ttgtatatgt cgttattaag tcattttggt acggttctgc atccgacatt
    12241 ctggtgttaa ctcgctgttt tgttagcgaa ttaacgctat tgccgttgtg gttattttat
    12301 agtgtattta ttctcagaga atatatatta ctgaaataaa aaacacaaag aataaccaaa
    12361 ggaaattatt tgaaacgtaa aaggtatata tttttaatct gtaaaattta aaatcacctc
    12421 ctctggattg aatcaacttg ctctatttaa gacgattgtt tttacatttt caccacctgt
    12481 atcaggtggt gtttttttat tggcggcaat gcctttatct tatttttatt acgttgtaaa
    12541 tatggctatt cctaaatgaa atttgatttg ctcagtttat ttgtgatgtc aggggagtgc
    12601 ttgccatgag agtcgtgata ttattattca tcatt
//
LOCUS       DAJTSI010000113        12499 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000113 DAJTSI010000000
VERSION     DAJTSI010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12499
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.113"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..726)
                     /locus_tag="OO327_002925"
     CDS             complement(<1..726)
                     /locus_tag="OO327_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCR6019385.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFDQAEPHAEKMPTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPTNVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRHLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKEEGRL"
     gene            complement(742..1074)
                     /gene="cusF"
                     /locus_tag="OO327_002926"
     CDS             complement(742..1074)
                     /gene="cusF"
                     /locus_tag="OO327_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCR6019386.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1098..2480)
                     /gene="cusC"
                     /locus_tag="OO327_002927"
     CDS             complement(1098..2480)
                     /gene="cusC"
                     /locus_tag="OO327_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCR6019387.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            2637..3320
                     /gene="cusR"
                     /locus_tag="OO327_002928"
     CDS             2637..3320
                     /gene="cusR"
                     /locus_tag="OO327_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCR6019388.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3310..4752
                     /gene="cusS"
                     /locus_tag="OO327_002929"
     CDS             3310..4752
                     /gene="cusS"
                     /locus_tag="OO327_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCR6019389.1"
                     /translation="MVSKPFQRPFSLATRLTFFISQATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVAPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            4902..7139
                     /gene="nrfB"
                     /locus_tag="OO327_002930"
     CDS             4902..7139
                     /gene="nrfB"
                     /locus_tag="OO327_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HCR6019390.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            7126..10094
                     /gene="nfrA"
                     /locus_tag="OO327_002931"
                     /pseudo
     CDS             7126..10094
                     /gene="nfrA"
                     /locus_tag="OO327_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10095..10985
                     /locus_tag="OO327_002932"
     CDS             10095..10985
                     /locus_tag="OO327_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCR6019391.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11168..11929
                     /gene="envY"
                     /locus_tag="OO327_002933"
     CDS             11168..11929
                     /gene="envY"
                     /locus_tag="OO327_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR6019392.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(11975..12051)
                     /locus_tag="OO327_002934"
     tRNA            complement(11975..12051)
                     /locus_tag="OO327_002934"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12015..12017),aa:Arg,seq:tct)
     gene            12294..>12499
                     /locus_tag="OO327_002935"
     CDS             12294..>12499
                     /locus_tag="OO327_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR6019393.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3030 a         3266 c         3186 g         3017 t
ORIGIN      
        1 caacctacct tcctctttcg cgaccacgtt atctttggcg atattcattc ccgcgcgcag
       61 atcaaacgcg gtgatcacgc catcaatggg cgctttgagc gtaaagcgag tctggatttt
      121 ttgcgtggcg atcaggtggc gaatatccgc ctccggcatt cccgccagtc gcagccgctc
      181 aagaatgcct tcagtctggg tcgccgtacc gccggtttcg cgcaacagta aatactcact
      241 ctgcgcttcc acccagtcgg gaatggtcag gtcgagaagc ggtgtgccct tttgtacttt
      301 atcgcccacg gtaagcggat acaccttgtc gataaacccg gcagcgcgag cctgcacaat
      361 ggcatactga tactcgttgt aactgacatt cgtcgggaaa ctctgggcaa aagtcagcgg
      421 cccgcgcgtg acggtagccg ttttcacccc cagattctgc gtctgagtcg ggtcaatgcg
      481 cacaccagac gcagaactct cttcatcggc atatttcggc accagatcca tatccataaa
      541 cggcgattta cctggtttat cgaaccgcgt attgggatac atcgggtcgt accagaataa
      601 gattttacgt tctgcggtcg gcattttttc tgcgtgcggt tccgcctggt caaaccaggt
      661 aaaacctgcc gctgaaataa taccgcccgc gatcatgctg ccgataataa gcgcgatttt
      721 tttcatctca ttaaacctgg gttactggct gactttaata tcctgtaata aagaaaggtt
      781 gccctgctgg acaaaattaa acgccacttt gtcgccggtt ttaatttcac tcattttcgt
      841 ctgcggggtg atggtaaagc gcatggtcat ctccggccag ttgacggcag caatcggatc
      901 gtgatggatg gtgatttttt tgctttccag atcaacacct tttaccacac cggtggcact
      961 aatgacctgt ggttgtgctt cgctcatggt ttcatgatga tgttcgttag cctgggcatt
     1021 aaagccaata acggtaaaca gactgaacat tgcgacttgc agtgcttttt tcatttattc
     1081 tctcctggag ttaaaaatta ttgctgccaa ccgcccccta aggcggtata caaataaatt
     1141 tcgttaacct gacgggcata attcaggtca agtaaagttt gtcgggttgc aaataaagaa
     1201 cgctcggcat ccagcacttc cagataactt accgcgccgt gctgatataa tgcccgcgcc
     1261 cgttgcaggg taatttgcag cgacgccaga taacgctgct gggcgctgat ttgatcgtcc
     1321 aggctttgac gtaatgccag cgcatctgcc acctctttaa aggcgttctg gattttctgt
     1381 tcataattca ccaccgactg ctgctggcga atttcggcga tatccagatt ggcctggttg
     1441 cgtccggcat taaaaatggg gatctcaatt ttaggaataa aattccacat cccgctgcta
     1501 gcgttaaata atgacgacag atcgctgctg gcggttgata ttccgctggt caggctgata
     1561 gacgggaaaa atgccgcacg cgcagcacca atattagcat tagccgccat taacgcgtgt
     1621 tcagcttcca taatatccgg gcgctgcaat aagatttgcg acgacaagcc cgccggtaat
     1681 ttaacgcttt gcaggctgcc gctgtttact gtctgcgctt gcggtagctt gccgtagctt
     1741 cccaataaca gttgcaatgc attattcgcc tgcgccagtt ccccctgacg tttagcgatg
     1801 tcactgcggg tactttctat caccccgcga gcctgttcca gcgccagaac attgctgcta
     1861 ccggtcaaca gttgtttttc gacaaacgca tatgactgct gataattacg cagcgtttct
     1921 tcggctattt gcaattgcgc atacgccagc tgctgattga aatagctttg cgcgacatta
     1981 gaaaccagca aaatatgcac cgcgcgctgg gcttcctcag tggctaaata attttggcgc
     2041 tcggcttcgc tcatgttctt taagcgaccg aaaaaatcga gatcaaagct ggcgttaagg
     2101 ccagtcgaga actcccgcgt cgtggctgaa tcgcctttaa gattgccgct ccagctgccg
     2161 ctgccctcgc cattgagctg tgggtagcgg tcggcatcgg tcagacgata ttgcgcccgt
     2221 gcctcctgca ctttcagcgt cgccatgcgc aaatcccggt tattcaccag cgcctcgcta
     2281 atcagcgtct tcacctgatt atcaacaaaa aaggtgcgcc agcccacgtt ctggtagtta
     2341 tccgctgcgt taaccaggcc gttctggctg agtgagaact gctgcggtac gggcattacc
     2401 ggacgctgat aatccggtgc cagtgaacaa ccagttagcg caagggccac acaaaatggc
     2461 agaagtttac aaggagacat aggctcataa tttctggtga ttttatgtcg ccaactttac
     2521 tcgccaggct ctgattttcc ggtgacagga aaatgacaaa attgtcattt tgccaataag
     2581 cgattgccat ctgatcccgc tactctagaa ttgcccgggc aacatgcgga ggaaatatga
     2641 aactgttgat tgtcgaagat gaaaagaaaa ccggagaata cttgaccaaa gggttaaccg
     2701 aagccggttt tgtggtcgat ttggccgaca acgggctgaa tggctaccat ctggcgatga
     2761 ccggtgatta tgacctgata atcctcgata ttatgctgcc agacgtgaac ggctgggata
     2821 tcgtgcgcat gttgcgctcc gccaataaag ggatgccgat tctgttgctt accgcgcttg
     2881 gcaccattga acatcgcgtc aaggggctgg agttgggggc agatgactac ctggtgaaac
     2941 cgttcgcttt tgctgaactg ctggcgcggg tgcgcactct cctgcggcgc ggggcggcgg
     3001 tgattatcga aagtcagttt caggttgccg atctgatggt cgatctcgtc agccgcaaag
     3061 tcacccgcag cggcacgcgc atcactctga ccagtaaaga atttactctg ctggagtttt
     3121 ttctccgcca tcagggcgaa gtgctgcccc gctcgcttat cgcctcgcag gtatgggaca
     3181 tgaatttcga cagcgacact aacgccattg atgtggcggt gaagctgcta cgcggcaaaa
     3241 tcgacaacga ctttgagccg aagctgattc aaaccgtgcg cggcgtgggt tacatgcttg
     3301 aggtgccgga tggtcagtaa gccatttcag cgcccgtttt cgctggcaac ccgcctgacc
     3361 ttttttatca gccaggccac catcgcggcg tttttcgcct ttgcatggat cataatccac
     3421 tcagtaaaag tgcattttgc cgagcaggac attaatgatt taaaagagat tagcgccacc
     3481 cttgaacggg tactaaatca ccctgacgaa acgcaagccc gacgcttaat gacgctggaa
     3541 gatatcgtca gtggttattc caacgtgttg atttccctgg cagatagcca cggcaaaacg
     3601 gtgtatcact cccccggtgc gcctgatatc cgcgagtttg cacgtgatgc catacccgat
     3661 aaagacgcgc ggggtggcga ggtgtatctc ctttccggcc cgacgattat gatgccaggc
     3721 cacggtcacg ggcatatgga acacagcaac tggcggatga ttaacttgcc ggttggcccg
     3781 ttggtggacg gcaaaccgat ttatacgctc tacatcgcgc tgtcgatcga ttttcatctt
     3841 cattacataa atgatttgat gaataaactt attatgaccg catcggtaat cagcatcctg
     3901 atcgtcttta tcgtgctgtt ggcggtacat aaaggtcacg cgccgatccg cagcgtcagc
     3961 cgtcaaatcc agaatatcac ctcgaaagat ctcgacgttc gccttgaccc gcagaccgtg
     4021 ccgattgagc tggaacagct ggtactgtcg ttcaaccata tgatcgagcg tattgaggat
     4081 gtctttactc gccagtccaa tttctcagcg gatatcgccc acgaaattcg cacaccaatt
     4141 actaatctca ttacgcaaac ggaaatcgcc ctcagccagt cgcgcagcca gaaggagctg
     4201 gaagatgtgc tctactctaa tctcgaagag ctgacgcgaa tggcgaaaat ggtcagcgat
     4261 atgctgtttc tcgctcaggc cgataacaac cagctaatcc ccgaaaagaa aatgctcaac
     4321 ctggcggatg aagtcggcaa agtgttcgat tttttcgagg cgctagcgga agatcgcggc
     4381 gtggagttgc ggtttgttgg cgacaaacgt caggttgcgg gcgatccgtt gatgctgcgt
     4441 cgggcgctaa gcaacctgct ttctaatgcc cttcgttata cgccaaccag agagacaatt
     4501 gtagtacgct gtcagacggt cgatcaccag gtgcaagttt ccgtcgaaaa ccccggtacg
     4561 cccattgcgc ccgagcactt accgcgattg tttgaccgtt tctatcgcgt tgccccctcc
     4621 cgccagcgaa aaggtgaagg tagcggtatt gggctggcga tagtgaaatc gattgttgtc
     4681 gcgcataaag gcacggttgc ggtaacgtca gatgcgcggg ggacaaggtt tgttatcaca
     4741 ttacccgctt aattattcag caaacggcaa cttttataac cagtgtaaaa ataacgtgcc
     4801 gcaatagtca tacatattaa tggtaaaaag ctgtcacaat tcataaaaaa ccttaatata
     4861 cgccacccta aacataacca gcgttaatgt aaggtttttg tgtggactgg cttcttgatg
     4921 tttttgctac ctggctctac ggcttaaaag taatcgcgat aacgttagcg gtcatcatgt
     4981 tcatcagcgg gctggacgat ttttttattg atgtcgtcta ctgggtacgc cgcattaaac
     5041 gcaagttgag tgtttatcgc cgctacccgc gaatgagtta ccgcgaactg tataaaccag
     5101 atgaaaaacc gttagcgatt atggttccgg cgtggaatga aacgggcgtc atcggcaata
     5161 tggccgagct ggcggcgacc acgctcgact acgaaaacta tcatatcttt gttggcacct
     5221 accccaacga ccccgatact caacgtgatg ttgacgaagt gtgcgctcgc tttccgaacg
     5281 tgcataaggt agtctgcgcg cgtcctggtc ccaccagcaa agccgactgc ctgaacaacg
     5341 tgctggacgc catcacccag tttgaacgta gcgccaattt cgcttttgct ggttttattc
     5401 tgcatgacgc cgaagatgtg atttcaccga tggaattgcg tctgttcaac tatctggtcg
     5461 agcgtaaaga tctgattcag atcccagtgt atccgtttga acgcgaatgg acgcacttca
     5521 ccagcatgac ttacattgat gagttttcag aactgcatgg caaagatgtt ccggtgcgtg
     5581 aagccctcgc cggacaagtg cccagcgcag gcgtcggcac ctgtttcagc cgccgcgccg
     5641 tgaccgcact gttagctgac ggtaacggta ttgctttcga cgtgcagagt cttactgaag
     5701 attacgacat tggcttccgc ctgaaagaaa aaggtatgac ggaaattttt gtccgttttc
     5761 cggtggtgga cgaagccaaa gaacgcgagc agcgtaaatt tttacagcac gcacggacgt
     5821 caaacatgat ctgcgtgcgc gaatatttcc ccgatacctt ttcgactgcg gttcgacaaa
     5881 aatctcgctg gatcatcggc attgttttcc agggctttaa aacccataaa tggacctcca
     5941 gcctgacgct gaactacttt ctctggcgcg accgcaaagg ggcaatcagt aactttgtca
     6001 gcttcctcgc gatgctggtg atgatccagc ttttgctgtt gctggcgtat gaaagtttgt
     6061 ggcccgatgc ctggcatttc ctttctattt ttagcggcag cgcatggtta atgaccctgc
     6121 tgtggctaaa ctttggcttg atggttaacc gcatcgtgca gcgagtgatt ttcgtcactg
     6181 gctactacgg cctcacgcag ggcctgcttt ccgtcctgcg tcttttctgg ggcaacctga
     6241 ttaactttat ggctaactgg cgcgtgctga aacaggttct tcagcacggc gatccgcgtc
     6301 gcgtcgcgtg ggataaaaca acgcatgact tccccagcgt cactggcgat acccgctcgt
     6361 tgcgcccgtt aggtcaaatt ctgctggaaa atcaggtcat cactgaagaa caactcgata
     6421 cagcactgcg taatcgcgtc gaaggtctac gcctgggcgg ttcaatgctg atgcaggggc
     6481 tgattagcgc cgagcagctg gcacaggcgc tggcagagca aaacggcgtg gcgtgggaat
     6541 ccatcgatgc ctggcagatc ccgtcctcgc tgattgccga aatgccagcc tccgtagcgc
     6601 tgcattatgc agtactgccg ctgcgtctgg aaaatgatga gttaattgtc ggcagtgaag
     6661 atggtattga cccggtttcg ctggcggccc tgacgcgtaa agtcggacgc aaagtgcgtt
     6721 acgtcattgt tctgcgggga caaattgtca cagggttacg ccactggtat gcacgccgac
     6781 gcggtcacga tccgcgggca atgttgtaca atgcggttca gcatcagtgg ctcacggaac
     6841 agcagaccgg tgaaatctgg cggcaatatg tgccgcatca gttcctgttc gccgaaatac
     6901 tgaccacgct cggtcatatt aatcgttcag caattaacgt gttgttattg cgccatgaac
     6961 gcagttctct gccgctcggc aagtttttgg tcaccgaagg cgttatcagc caggaaacgt
     7021 tggatcgcgt cctgacaatt caacgcgaat tacaagtttc gatgcaatca ctattactca
     7081 aagcaggttt aaacacagaa caggttgcgc aactggagtc cgaaaatgaa ggagaataac
     7141 cttaatcgcg tcatcggatg gtctggttta ctgctgacgt ctttattgag taccagcgca
     7201 ctcgcagaca atatcggcac cagcgcagaa gagctggggc tgagcgatta tcgccatttt
     7261 gttatttatc cccgtctcga taaggcgctg aaggcacaga aaaataacga cgaagcaacc
     7321 gccatccgcg aatttgaata tatacaccaa caggtgccgg ataatattcc gctgacttta
     7381 taccttgcgg aagcctatcg tcattttggt catgatgacc gggcgcggct gttgcttgag
     7441 gatcaactga aacgtcaccc aggagatgcc cgacttgagc gcagtctggc ggctattcca
     7501 gttgaagtga aaagcgttac gactgttgaa gaactgcttg cccagcaaaa agcgggcgac
     7561 gctgcgccga ccctgcgttg tcgcagtgaa gtcgggcaga atgccctgcg gctggcacaa
     7621 ttacctgtcg ccagagcgca actgaacgat gcgacgtttg ctgcatcgcc ggaaggaaaa
     7681 acgctgcgaa ccgatctgct gcaacgggca atctacctga aacaatgatc ccaggcagat
     7741 acgctataca atgaagcacg ccagcagaac acattaagcg cggcagaacg ccgtcagtgg
     7801 tttgacgtgc ttcttgccgg gcagctggac gatcggatcc tggcactgca atcacagggg
     7861 atcttcaccg atcctcagtc atatattact tacgcgaccg cgctggctta tcgtggcgaa
     7921 aaagcacgcc tccagcatta tctcattgaa aataagccgc tgtttaccac gggcgcacaa
     7981 gagaaaagtt ggctctatct gttatctaaa tacagcgcca accccgttca ggcattggcg
     8041 aattatacgg tgcagtttgc cgataaccgc cagtatgttg ttggcgcgac gctaccggtg
     8101 ctgttaaaag aaggtcagta cgacgcagcg caaaaactgc tcgccaccct ccccgccaat
     8161 gaaatgcttg aggagcgtta tgctgtcagc gtggcgaccc gtaacaaggc tgaatctctg
     8221 cgtctggcac gattgctgta tcagcaagaa ccgacaaatc ttccccgcct ggatcaactg
     8281 acctggcaac tgatgcagaa cgagcagtca cgcgaagctg ccgatttatt gctgcaacgc
     8341 tatcctttcc agggcgatgc gcgtgtcagc cagactttaa tggcgcgact ggcgtctctg
     8401 ctggaaagtc atccttacct agcaacgccg gcgaaggtgg cgattttatc gaaaccctta
     8461 ccgctggcgg agcaacgtca gtggcaaagt cagttgccgg gtattgcaga taattgcccg
     8521 gcaatagttc gcttgctggg cgatatgtcg ccttcctacg atgctgccgc ctggaaccgt
     8581 ctggcaaagt gttatcggga cacgctaccc ggtgtggcgt tgtatgcatg gcttcaggcc
     8641 gaacaacgac aaccgagcgc ctggcaacat cgtgcggtag cctatcaggc gtatcaggtt
     8701 gaggactacg ccaccgcact ggcggcctgg cagaaaatca gtcttcacga catgagcaat
     8761 gatgatctgc tcgccgccgc caacaccgct caggcagcgg gaaatggcgc agctcgcgat
     8821 cgctggcttc aacaggcaga acaacgtgag ctgggaaaca atgctcaata ctggtggctg
     8881 catgcgcgac gttatatccc agctcagcca gaactcgcac tgaacgatct cacgcgctca
     8941 atcaatattg ccccttctgc caactcttac gttgcgcggg cgacaattta tcgccaacgt
     9001 cataatgttc cggccgcggt gagtgatttg cgcgccgcgc tggaactgga accaaataat
     9061 agcaacaccc aggcagcgct tggttacgcc ttgtgggata gcggtgatat cgcacagtcg
     9121 cgggaaatgc tcgaacaggc gcataaaggg cttccggacg atccggcact gatccgacaa
     9181 ctggcctacg tgaaccagcg tctggatgac atgcctgcga cgcagcacta cgcccggctc
     9241 gtgattgatg acattgataa tcaggcataa ccccactgac cccagagcaa aatcagcaac
     9301 gcttcaattt ccgccgtttg catgaggagg tcggtcgccg ctggacgttc agtttcgatt
     9361 cttccatcgg cttgcgttcc ggcgcaatga gtaccgctaa caataatgtc ggcggtgcag
     9421 cgcctgggaa aagctatcgt agctacggac aactggaagc cgagtaccgc atcggacgca
     9481 atatgctgct ggaaggcgac ctgctctcag tttatagccg cgtctttgcc gataccggag
     9541 aaaacggggt gatgatgccg gtgaaaaatc cgatgtccgg caccggtctg cgctggaagc
     9601 cgctgcgcga tcagatcttt ttcctcgccg tcgaacagca gttgccgctg aacgggcaaa
     9661 atggcgcatc cgataccatg ctgcgcgcca gcgcctcatt ctttaatggc ggcaaataca
     9721 gcgacgaatg gcacccgaac ggttcaggct ggtttgccca aaacctgtac ctcgatgcgg
     9781 cgcaatatat ccgccaggat attcaggcgt ggacggcaga ttatcgcgtc agctggcatc
     9841 agaaggtagc taacggacag actattgagc cttacgctca cgttcaggac aacggctatc
     9901 gtgataaagg cactcagggc gcgcagcttg gcggtgtcgg ggtccgctgg aatatttgga
     9961 ccggcgagac gcactacgac gcctggccgc acaaagtcag tctcggcgtc gagtatcaac
    10021 atacctttaa ggcgattaat caacgtaacg gagagcgcaa caacgcgttt ctcaccattg
    10081 gagtgcactg gtaaatgcgt aagttcattt tcgtattgct gacactgctt ttggtcagcc
    10141 ctttttcctt tgcgatgaaa ggtattatct ggcaaccaca aaaccgagat agtcaggtta
    10201 ccgataccca gtggcagggg ctgatgagtc agttacgttt tcaaggcttc gataccctgg
    10261 ttttgcaatg gacccgttac ggcgatgcat ttacccagcc agaacagcgc acgttattgt
    10321 ttaagcgtgc cgcagctgcg caacagactg gcctgaagct gattgtcggg ctgaacgccg
    10381 atccggaatt ttttatgcac cagaaacagt cgtctgcagc gctggaaagc tatcttaatc
    10441 gcctgctggc tgccgatctc cagcaagcca gattatggag cgccgcgcct ggcataacgc
    10501 cggatggctg gtacatcagc gcggaaattg acgacctgaa ctggcgcagc gaagccgccc
    10561 gtcaaccttt gctaacatgg ttaaacaacg cgcagcggct gattagcgat gtttcagcaa
    10621 aaccggttta tatcagtagt tttttcgccg gaaacatgtc gcccgatggc tatcgccaac
    10681 tgctggaaca agttaaagca accggagtta atgtctgggt acaggatggc agcggcgtgg
    10741 ataaacttac cgctgaacag cgtgaacgtt atctacaggc cagcgccgat tgccaaagct
    10801 ccgccccagc aagcggcatt gtttatgaac tgtttgtcgc cggcaaaggc aaaaccttta
    10861 cagcgaaacc gaaaccggac gcagaaattg cctcgctgtt agcgaaacgt tcctcttgcg
    10921 gtaaagacat gctctatttc tctctgcgct atttgcccgt cgcgcacggc attctcgagt
    10981 attaaatccc ctccaggtaa gtcgggtacg acctggctta cctctttcgc tctcgtcaga
    11041 attccatcaa gattaattgc taaaaagcat gctaaagaaa gaagatcgtt atcggcgaac
    11101 gggccgatca ctcatgcagg aagcatagat cctttgagcg gagtttctga tatctgagga
    11161 gtgcgaaatg caattgagca gcagtgaacc ttgcgtggtg atcctgaccg aaaaagaggt
    11221 agaggtaagc gtcaataacc atgctacgtt tacccttccg aaaaactacc tggccgcctt
    11281 cgcgtgcaac aataacgtca ttgaactctc aacgttaaat cacgtattaa tcacccacat
    11341 caaccgtaac atcatcaacg attatctgtt gtttttaaat aagaaattaa cctgtgtaaa
    11401 gccctggtcg cggctagcaa ccccggttat cgcttgtcat agccgtacac cggaagtgtt
    11461 ccggctagcc accaaccaca gcaagcagca atccagcaaa ccctgcgaag cggagttgac
    11521 gcgcgcatta ctttttaccg tattgtctaa ctttcttgag caatcgcggt ttattgccct
    11581 actgatgtat atcttacgca gcagcgtccg cgacagcgtc tgccgcatta ttcaaagcga
    11641 tattcagcat tactggaatc tgcgaattgt cgccagttcg ctatgtttaa gccccagcct
    11701 gctcaaaaag aaattaaaaa acgaaaatac cagctatagc cagattgtca cagagtgtcg
    11761 tatgcgttac gccgtacaga tgttattgat ggataacaaa aatatcactc aggtggcgca
    11821 attatgtggc tatagcagca cgtcgtactt tatctctgtt tttaaggcgt tttacagcct
    11881 gacaccgttg aattatctcg ccaaacagcg acaaaaagtg atgtggtgaa gggcaaagcg
    11941 gaaacggata agacgggcat aaatgaagaa gaaatggcgc gccctgcagg attcgaacct
    12001 gcggcccacg acttagaagg tcgttgctct atccaactga gctaagggcg cgttgatacc
    12061 gcaatgcggt gtaatcgcgt gaattatacg gtcaaccctt gctgagtcaa tggcttttga
    12121 tctggttgct gaacaagtga acgaccgcgt ctgattttct gatttatttc gctatagcgg
    12181 caaacaaacg cacaccgctg cgcgtctgaa tcaagaaaac ccgtattttc atgtatcaaa
    12241 gtacaattta ccgccctaac ggaaaattgt ccgctcctat gagactggta actatgaaac
    12301 caacgtcggt gatcattatg gatactcatc ctatcatcag aatgtctatt gaagttctgt
    12361 tgcaaaaaaa cagtgaattg cagattgtcc tgaaaacgga tgattaccgc ataaccatcg
    12421 attatctccg aacccgtcct gttgatttaa tcattatgga tatagacttg cccggaacag
    12481 acggttttac cttcctgaa
//
LOCUS       DAJTSI010000114        12365 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000114 DAJTSI010000000
VERSION     DAJTSI010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.114"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1038
                     /locus_tag="OO327_002936"
     CDS             <1..1038
                     /locus_tag="OO327_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR6019394.1"
                     /translation="FFPNLVDHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIH
                     GVWFDEEPPYSIYGEGLTRTNKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMT
                     IYDAEHYTDEQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHF
                     YVIGGMDFGWDHPQAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTA
                     WPHDGNQHEKGGGEQLKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFK
                     VFNTCEPFFEEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKI
                     PAPIRPIARRT"
     gene            1039..3204
                     /locus_tag="OO327_002937"
     CDS             1039..3204
                     /locus_tag="OO327_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR6019395.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQFDVVRPEVRKLVAEMRRNPVDVLFRPKDGANPDAADVLMGMYRTDMR
                     HNTAKIAVNVGVREQIESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDAN
                     SKQMDKSDAKHCTVINALSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVY
                     VAEYYEVEEEKEKVFIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRV
                     YKSIITCTQILKDREKIAGEHIPIVPVYGEWSFAGDKECYEGVVRLTKDGQRLRNMIM
                     SFNADIVARSPKKKPTFFPEQIEGYEYMYGGNDDYPYYLQNRTDENGNDLPIGPISYM
                     ENPEVPQANAYMLEAATNAVKEVASLGVDAQAANGQVAFDTVNQLNMRADLETYVFQD
                     NLATAMRRDGEIYASMVNDIYDVPRHVTLTLEDGSEKDVQLYAQVVDYQSGNVVTLND
                     IRGRYECYTDVGPSFQSMKEQNRAEIQELLTKVPQGTPEFQMLMLQYFTLLDGKGVEM
                     MREYANKQLVMMGLKKPETPEEMEMVQQAQQQPQQPSAEQIQAQGILLQGQAELLKAE
                     NQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMDLDKQAELREYLRLVGQFQQQRSKDAR
                     ANAELLLKDADQTHSQRMDFANLMRQVQIPSGGVAETPQ"
     gene            3218..4129
                     /locus_tag="OO327_002938"
     CDS             3218..4129
                     /locus_tag="OO327_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HCR6019396.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VRKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            4129..5424
                     /locus_tag="OO327_002939"
     CDS             4129..5424
                     /locus_tag="OO327_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HCR6019397.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            5469..5699
                     /locus_tag="OO327_002940"
     CDS             5469..5699
                     /locus_tag="OO327_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019398.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            5677..6177
                     /locus_tag="OO327_002941"
     CDS             5677..6177
                     /locus_tag="OO327_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HCR6019399.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            6177..7595
                     /locus_tag="OO327_002942"
     CDS             6177..7595
                     /locus_tag="OO327_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HCR6019400.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSIANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            7595..8443
                     /locus_tag="OO327_002943"
     CDS             7595..8443
                     /locus_tag="OO327_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019401.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            8443..8898
                     /locus_tag="OO327_002944"
     CDS             8443..8898
                     /locus_tag="OO327_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCR6019402.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            8901..9593
                     /locus_tag="OO327_002945"
     CDS             8901..9593
                     /locus_tag="OO327_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR6019403.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            9603..10934
                     /locus_tag="OO327_002946"
     CDS             9603..10934
                     /locus_tag="OO327_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR6019404.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPHDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            10935..>12365
                     /locus_tag="OO327_002947"
     CDS             10935..>12365
                     /locus_tag="OO327_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR6019405.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSAL"
BASE COUNT         3314 a         3075 c         3253 g         2723 t
ORIGIN      
        1 ttctttccga accttgttga tcaccttctg gttaagcatc acacggctga tggcgttgaa
       61 gatggcattt caatctgcta cttcaagcca tactcgcaag gccgtgctcg ctggcagggt
      121 gacacaatcc acggcgtgtg gtttgacgaa gagccaccat acagcattta tggcgaaggt
      181 cttacccgta ccaacaaata cgggcaattc tcaattctga cgtttacccc gctgatgggg
      241 atgtctgacg ttgttaccaa gttcctgaag aaccccagca agtcgcagaa agtggtcaac
      301 atgaccatct atgacgctga gcactatacc gacgagcaga aagagcaaat catcgcatca
      361 tatcccgagc atgagagaga ggcgcgtgct cgcggtattc ctacgatggg tagtggtcga
      421 atcttccaga taccggaaga gacgattaag tgtcagccgt tcgagtgtcc tgatcacttc
      481 tacgtaattg gcgggatgga tttcggatgg gatcacccac aggcgcaagt tcagctttgg
      541 tgggataagg aggcagacac aatctacgtt tcacgcgtgt ggaaggcgaa agaaaaaaca
      601 gccgttcagg cgtggggagc cgttaaatca tgggcgcata aagtgccaac cgcatggcct
      661 catgatggaa accagcacga gaagggcggc ggtgagcagc tcaaagggca gtatgcagac
      721 gctggattta tgatgttgca ggagcatgcg acatggcctg atggcggtaa cgctgttgag
      781 ccaggcatca ctgaattgcg cgacatgatg ctcgatggtc gcttcaaagt attcaatacc
      841 tgtgagccat tctttgagga gttccgcctc tatcaccgtg atgaaaacgg gaaaatcgtc
      901 aagcttaacg acgacgtgct atccgccgtt cgctatgcat acatgatgcg ccgcttcgca
      961 aaaatgatgc gcgacatcaa aaaaccaaaa gagaaaaaga taccagcccc aatcaggccc
     1021 atcgcacgga gaacttaaat ggccgacgaa aacagactca attccattct gtgtaagttt
     1081 gacgcagact ggatggcgag cgatgaagcc agaaccgagg cgacaaatga cctgtatttt
     1141 agccgagtgt cgcaatggga tgactggcta tcaaactaca ctaccctgca atatcgcgga
     1201 caattcgatg ttgttcgccc ggaggtcagg aaactggtcg cagagatgcg ccggaaccct
     1261 gtcgacgttc tcttccgacc caaagacggc gctaatcctg atgcagccga tgtattgatg
     1321 gggatgtatc gtactgatat gcgccataac acggcaaaga ttgccgttaa cgttggcgtt
     1381 cgtgagcaga tagagtcagg cgttggtgca tggcgtctgg tcacgcagta cgaagacaac
     1441 gatccaacaa gcaacaatca ggtaatccga cgcctgccaa tccatgaagc ctgctcacac
     1501 gtcatatggg acgccaacag caagcagatg gataagagcg acgctaagca ctgcacggtg
     1561 attaacgcct tgtcgcgcaa tggctggaaa gagttcgcag aggattacgg tattgacccg
     1621 gataccctgc catctttcca gaatcctaac gatacatggc tattcccgtg ggtgtcgaat
     1681 gatgtcgtct acgtcgctga gtattacgag gtagaagagg agaaggagaa ggtcttcatc
     1741 taccgcgacc cgctgacagg tgagccggtc agctattacc agcaggatat caaagacgtc
     1801 atcgacgacc tggctaatcg tggattcatt aaggtagcag agcgcaaggt gaagcgtcgg
     1861 cgtgtgtata agtcgatcat cacctgtacg cagatactga aagaccgcga gaagatagcc
     1921 ggagagcata ttccaatcgt tccagtgtat ggcgaatggt cattcgctgg tgacaaggag
     1981 tgctacgagg gtgtggtaag gctgacgaaa gacggtcaac gccttcgtaa catgattatg
     2041 tcgttcaacg ccgatattgt tgctcggtca ccgaagaaga aaccgacctt cttccctgag
     2101 caaatcgaag gctacgaata catgtacggc ggaaatgatg actatccgta ctatctgcag
     2161 aacaggactg atgaaaacgg taacgacctg ccgattggtc caatctccta catggaaaac
     2221 cctgaagtgc cgcaagccaa cgcttacatg cttgaggctg ccaccaacgc agtgaaagaa
     2281 gtggctagtc ttggcgtgga tgcgcaggcg gcaaatggtc aggtcgcttt tgataccgtc
     2341 aatcaactga acatgcgggc agaccttgag acatacgtgt ttcaggataa cctggctacc
     2401 gcaatgcgac gtgatggcga gatttatgcc tcaatggtca acgatattta tgacgttcct
     2461 cgtcatgtaa cgctgacact tgaagatgga agcgagaaag acgttcaact ctatgcgcaa
     2521 gttgtcgatt accagtccgg caatgtggtc acactcaacg acattcgcgg tcgatatgag
     2581 tgctatacag acgttgggcc atccttccag agtatgaagg aacagaaccg cgcagagatt
     2641 caggagttgc tcaccaaggt tccgcaaggt actccagagt tccagatgct gatgctgcaa
     2701 tacttcacgc tgcttgacgg taaaggcgtc gagatgatgc gagagtacgc gaacaagcaa
     2761 ctggtgatga tggggctgaa gaaaccagaa acacctgaag agatggagat ggtgcagcag
     2821 gcacaacaac agccgcagca gccatcagca gagcaaattc aggcgcaggg tatccttctg
     2881 caaggtcagg ctgaattgct caaggcagag aaccaacagg cgcagattca ggttgaagct
     2941 gccaaggttg aagcccaaaa ccaactcaac gccgcgaaga ttgcagaaat attcaacaat
     3001 atggacctcg acaagcaggc agaactgcgt gagtacctca ggctcgtagg tcaattccag
     3061 caacagcgca gcaaagatgc tcgtgctaac gctgagctgc ttcttaaaga tgcagaccag
     3121 actcattcac aacgcatgga tttcgcgaat cttatgcgtc aagttcaaat cccctccggc
     3181 ggagtagccg agacacctca ataagagaga gttaatcatg gaccaaacca ccgacattca
     3241 ggcttctgaa gaattaaccc tgcccggcaa tcatgcagcg gcatctgctg atggcttagt
     3301 tgtcgataat gccaacgaca gcgcaggtca ggaagaaggc tttgagattg tccggaaaga
     3361 cgatgagaaa ccaaaacaag acccggcaac taatgctgaa tttgcccgtc gccgcatcga
     3421 acgcaaacgc cagcgtgagc ttgagcagca gatggaagcg gttaagcgtg gagagttgcc
     3481 ggagcacctg cgggtgaacc ctgagttacc aaaacaacca gaccctaacg attatctttc
     3541 cgaagatgca ctggctaagt acgactatga ccagagccgc gcactggctg ccttccagca
     3601 ggcaaacagt gaatggcaga tcaaggctat ggacgcacga agccaggctg tcgccgagca
     3661 gggtcgcaaa actcaggagt tcacccagca atcagcgcaa tacgtcgagg cggcccgtaa
     3721 gcactacgag gcagcggaaa agctcaatat ccctgactat caggagaaag aggatgcatt
     3781 catgcaactg gtgccgccag cagtcggtgc cgacatcatg cgcctcttcc cggagaaatc
     3841 cgccgctctc atgtatcacc ttggtgctaa tcctgagaaa acacgccagt tgctggcgat
     3901 ggacgggcaa tccgcgctga ttgaactcac tcgactgtca gaacgtttaa ctctcaagcc
     3961 tcgagccaag cctgtttcag aagccccgtt acctgatgaa cccattcagg gacatgctgt
     4021 tgctgcaaat atctctgcga ttgaaaagca gatggaggcg gcagcaaaca aaggggatgt
     4081 agagacgtac cgcaagctca aggcgcaact gaataaagga attcgataat ggcattaaat
     4141 gaaggtcaac tggtcacgta tgctctggat gaaatcatcg gaaccgtcca gaacctgacg
     4201 ccaatggcgt caaaagtgac aaaatacccc cctccggcag aatccatgca gcgttcaagc
     4261 aacaccgtgt ggatgcctgt tgagcaggaa gcgccaaccc agactggctg ggatttaact
     4321 ggcaacgcaa ccgggattct ggaactctcc gtgaagtgca acatgggcga tccggataac
     4381 gatttcttcg agcttcgtgc agatgacctg cgtgatgagc gttcttaccg tcgccgcatc
     4441 caggcatccg ccaaaaaact ggcgaataac attgagtcag cgattgccaa acaggcaact
     4501 gaaatgggct cgcttgttgt tcacgatacc cgcgcaattg gtccatcgac tggcctgcct
     4561 ggctgggatt ttgtgtctga tgcagagcga ctgatgttct cccgtgagct aaaccgcgac
     4621 atgggcatca gttacttcct gaaccctgac gattaccgca aagcaggccg caacctggta
     4681 gatggtgaca tcttcgggcg cgttcctgaa gaagcatatc gaaacggtac tattcagcgt
     4741 cagattgctg gctttgatga aattcttcgc tcaccgaaac ttccggcagt taccaagtca
     4801 accgcgactg gtgtaactgt ttctggtgcg cagaagttta agccgcaggc atacaccctt
     4861 gataccgatg gtaacaaaga gaacgtcgac aaccgtgttg caacggtgac cgtatcctcc
     4921 accaccggat ttaagcgcgg cgacaaaatc agcttcattg gtgtgaagtt cctgtctcag
     4981 atggcgaaga atgtgctgac tgatgacgcg actttctcaa tcacccgtgt gatcgatggt
     5041 actcacatcg aaatcacgcc gaagccgatt gcgctggatg acgcgtcact gacaaaagaa
     5101 gagaaggctt acgctaacgt aaacacctct cttgctgata ccactccggt aaacgttctg
     5161 aacgtggcaa caaccaccgc taacgtgttc tgggctgatg actcaatccg cctgctgtct
     5221 cagccgatcc cggtaaccca tgaactgttt gctggcatga aaacgtcttc cttcagcatt
     5281 ccaggtattg gtgttaacgg catcttcgca acgcagggtg atatcaacac tctgtctggt
     5341 aagtgccgta ttgctgtgtg gtattcagca tgtgctgtac gaccagaggc aattggtgtt
     5401 ggtctgccta accagaccgc gtgataacca gagggagctt cggctcccgt ttttatctgg
     5461 agacaagcat gacacacatg atctttcgtc atggcgacat gaagaagtgg aaaggcgttg
     5521 gctacgactt tgaaatcgtg aaagccgaag agcttcagga atatctggat gctggctggt
     5581 tttcacatcc tgatgacctt ttgaaggatg ttgcagagcc agagccagaa gaaaaacagc
     5641 gtaaaaagcc tggtcgaaaa cctaaggcgg cagcagatga acctgacaac gaagggtgat
     5701 ttagttcttg cggcattacg taagctcggt gttgcatcaa atgccacgtt aaccgatgtc
     5761 gaaccgcagt ctatggaaga cggcgtcaac gaccttgaaa tgatgatggc tgaatggctt
     5821 ggcggtgatg cgtcacctgg tatcaacgtt ggctacattt ttgctgatgc agatgttgct
     5881 ccggatccgg gcgatgagca cggtttatca aataacgcta tcaatgccgt cattttcaac
     5941 cttgcctgcc gaattgctcc agattatgcg ctggaagcgt ctgcaaaact tataaccact
     6001 gccagatacg ggaaagagcg actcgtcaaa ctgtctgcaa tggacagggc aaaagcagct
     6061 aaatgtaagt ccggttatcc aaaccgtatg cctgttggta gtggtaacca gttggcgaag
     6121 tggaatggtt ggaattactt ccaccgaaag gaaccttgcg ataacgggag cgaataatgc
     6181 ctattcagca acttccgctt atgaaaggtg tcggcaaaga cttccgaaac gccgactata
     6241 tcgactatct gccagtgaat atgctggcta cacccaaaga aatcctgaac agcagcggat
     6301 atcttcgctc attcccgggc attgccaaac gttctgacgt gaacggtgta tctcgcggcg
     6361 tcgagtacaa catggcgcag aatgctgttt atcgcgtgtg tggcggcaag ctgtacaaag
     6421 gagaaagtga agtcggtgat gttgccggaa gtggtcgtgt atcaatggcg catggtcgga
     6481 catcacaggc ggtaggcgtt aatggtcaac tggtcgagta tcgctatgat ggcacggtca
     6541 aaaccgtctc aaactggcct acagacagtg gattcactca gtatgagtta ggttcagttc
     6601 gcgacattac gcgcttacgt gggcgttatg cgtggtcaaa agacggaact gattcatggt
     6661 ttatcactga ccttgaagac gaatctcatc ctgaccgtta cagcgcacaa tatcgtgccg
     6721 agtctcagcc tgacggcatc atcggtatcg ggacatggcg agacttcatc gtctgctttg
     6781 gttcatcgac tattgaatat ttctccctga ctggcgcaac caccgttggt gccgctttgt
     6841 atgttgcaca accatcgctg atggtgcaga aaggcattgc cggaacttac tgcaaaacgc
     6901 cattcgctga ttcgtatgcg tttatcagca atccggcaac gggtgcgccg tctgtgtata
     6961 tcatcgactc cggtcaggtg tcaccaattg ccagcgcgag cattgagaaa atcctccgct
     7021 cctacactgc tgatgaactg gctgatggtg tgatggaatc gctgcgattt gatgctcatg
     7081 agttgctgat tatccacctt ccgcgtcacg tcctcgtgta cgacgcatct tcaatcgcca
     7141 atggcccaca atggtgtgtg ctgaaaacag gtctgtatga cgatgtgtac cgcgctatcg
     7201 acttcgttta cgaaggcaat cagataacgt gcggcgataa gctggaatct gttaccggga
     7261 aactgcaatt cgatatcagc agccagtacg acaagcaaca ggaacacctg ctgtttactc
     7321 cactcttcaa agcggataac gccagagtgt tcgaccttga ggttgaatcg tcaactggcg
     7381 ttgcgcagta cgccgaccgc ctttttctct ctgcaaccac tgactgcatc aattacgggc
     7441 gtgagcagat gattgagcag aatgaaccgt tcgtttacga caagcgtgtt ttgtggaagc
     7501 gagtagggcg catcaggaaa aatgtcggct tcaaattgcg cgttatcacg aagtcacctg
     7561 tcactctgtc tggcgctcag ataaggattg agtaatggct gattcgaatc tcaatgtgcc
     7621 ggtaatcatc caagctacgc ggctcgatac atcagtcctt ccacgcaata tcttctcgca
     7681 gtcgtatctg ctttacgtta tcgcacaggg cactgatgtt ggtaacgtgg ctaacaaagc
     7741 caacgaggcc ggacagggcg cttatgatgc acaggtcagg aacgatgagc aggatgtgat
     7801 tctcgctgac catgagcagc gaatttctgc tgcggaagca acgcttgtta atcatgagga
     7861 gcgaatcaga caggcagaat caactcttca ggaccatgaa acacgaatag ctcagaatga
     7921 aagcgatatt tcgtcgcttg acacaagagt tcagtcgctg gagtcgcagg tttcagacca
     7981 tgaaacgcgc atcgatgctc tggagtatgc aaccacacgc aagaagtcag aggttgttta
     8041 ctctggtgta tctgtaacca tcccgacagc gccgaccaac cttgttagcc tgctgaaaac
     8101 gctcacaccg tcatccggct cgttggcacc attcttcgac accgttaaca acaagatggt
     8161 tgtgttcaac gagaacaaaa ccctgctctt caagttgtcg attgtcggca cgtggcccag
     8221 cggaaccgcc aacaggtcaa tgcagctaac cttttccggc tctgttcctg acacactggt
     8281 cagcagtcgt aatgcggcga caacaaccga caacatcctg ttagctacgt tcttcagcgt
     8341 ggataaagac ggctttcttg ccacaaatgg cagtacgtta accattcagt cgaatggtgc
     8401 ggcgtttact gccacaacca tcaagataat cgcggagcag tgatgattac attcaaacca
     8461 acgcgaaaca tcgacctgat agaagcagtc ggaaatcacc ctgacattat cgccgggagc
     8521 aacaacggtg atggatacga ctacaaacct gaatgccgtt actttgaggt gaacgtgcac
     8581 ggtcagtttg gcggcattgt ttactatcag gagattcagc cgctgacatt agattgccac
     8641 gccatgtacc tgccagagat tcgcggcttc agcaaggaaa ttgggctggc gttctggcga
     8701 tacattctga ctaacaccac cgttcagtgc gtaacatcgt tcgctgcacg caaattccga
     8761 cacggtcaga tgtactgcgc aatgattggc cttaagcgtg taggaaccat caagaaatac
     8821 ttcaaaggcg tggatgacgt gactttttac agcgccacac gcgaagaact aatcgacttc
     8881 ctgaatcacg ggagatagcc atgttatatg catttaagct gggcagaaaa ctgcgcggcg
     8941 aggaacctta ttgccctgaa aaaggcggga aaggtggcag ttctgataaa agcgcaaagt
     9001 atgcagcaga agcccagaag tatgctgcag acctgcaaaa tcagcagtgg cagacgatca
     9061 tgaaaaacct tgctccgttc acgcctcttg cggagcagta tgttaaccag cttcagaacc
     9121 tttccagttt agaaggtcag gggcaggcac ttaatcagta ttacaactct aagcagtata
     9181 aagaccttgc agggcaggcg cgttaccaga gtcttgctgc tgcggaggcg acgggtggac
     9241 ttggttcgac agccacaagc aatcaactgg ctacgatcgc gccgacactc ggtcagtctt
     9301 ggttatcaaa tcagatgagc aattacaaca atctggcaaa cgttgggctt ggtgcgctgc
     9361 aaggtcaggc aaacgccggg cagacgtacg ccaacaacat gagcagcatt gcacagcaaa
     9421 gcgcagctct tgccgctgct aatgccaata aaccatcaag tcttcagact gcaattagcg
     9481 gtggcacgtc tggtgcgatt gccggtgcag gtcttgccag ccttttggga acatcaacgc
     9541 cttggggcgc tggtattggt gctggtatcg gattgcttgg ctcgttgttt taagggggaa
     9601 tcatggctac ttggcaagga acaaacggcg gattgttggc tggtatcggc ggcgtcaact
     9661 caaacgctcc gagcgtaaat gacatcggca atacgcttca gcttatcagg cagaacaatg
     9721 atattgagcg ttcaggcgct aacaatgttg ggctgactgc tttgcaaggt ctttcaggta
     9781 ttgcgggtgt ttttcagcag gaaaagcagg ctcagcggca gaaagaattt cagcaggcgt
     9841 acgctaatgc ttatgcgtct ggtgatcgcg gtgctttgcg tcagttggct actcaatatc
     9901 cagaccagat tgaatccgtt cgtaaaggca tgggattcat tgatgaagag cagcgtaatt
     9961 ctatcggcac cttagcggct ggcgcacgcc ttgcgtcatc gtctccagaa gcaatgcaat
    10021 catggctgca aaacaacgcc ggtgagttag ctcgtgttgg cgttaatcct catgacgtcg
    10081 ctcagatgta ccaacagaac ccgcggcagt tcggcgaatt tgtcgatcac ctggggatga
    10141 acagtctcgg gcccgaaaaa tactttgacc tacaggataa aatgcagggt cgccaggtta
    10201 ccatgcgcgg tcaggatctg gattcgcaaa ccgccgctcg gaatcaggca atcacaatgc
    10261 gcggacaaga tatccaggcg aatttaggtc agcagcgcat taatctggac gcagaaacaa
    10321 accgcattaa caacgaaaat aagcgccttg accggatgct atcagcagaa actaacgacc
    10381 tgaagcgcca ggaaatacag agccgcatag cagccaacaa ccagcagttg cagcagaagc
    10441 agcaagcgct aaatgatggc tacaaagacg gcatcaacac cctcacaacc agcatgttca
    10501 ctctgaacga tatcgttagt tctccttcac ttaagagcat tacaggctta cgtggagtaa
    10561 tccccaacgt tccaggctca caggctgcag acactcaggc acgacttgat acctttaaat
    10621 cacaagcata cctgacagcg gttcaggcca tgcgaggcat gggcgcactt tctgatgccg
    10681 agggcaaaaa gctcgaccag gctgttggtt cgctgcagaa ctcgcagagc gaggagtcct
    10741 ttcgtcgcaa cgctggcgtc atcctgaaca cgctcaacca gaagcgtaat gaggcggttg
    10801 gtaagtacgt tcagcaaaac ggtatcaagc gagtggaagc gcctcaggct tctatagatt
    10861 acctgaagca gcaccccgag ctgtcaatcg acttcattaa tcgctacgga tatcttccat
    10921 ctttggggca gtaaatggct aattaccgtg atttgttaga gcaggctggc gcacgttacg
    10981 gtgtgccaga agggttgatg actgcactgg gtgccaagga gtcttcttac aaccctgccg
    11041 cagtaagctc cgccggggct gtaggattga ctcaggtcat gcctgggaca tggcgtgata
    11101 tgggttatac cgatgagcaa atgcaaaacc ccgaatatca ggctgacgct ggcgcgcgct
    11161 atctggcaaa gatgtaccag cagtttggta actggcgtga ctctcttcag gcttatcacg
    11221 acggtcccgg caacgttatg aaggcaaagc gtggtgaata tacgccagga cctgaaggcc
    11281 gcggttacgt tgatgatcgc tttgctcaat gggctggtga cccggtgaca gactcaacag
    11341 tcgaacagcg cgccacctct gcaaaggtac atcctcagca agaccctaac aacccgtttg
    11401 cacaactgga agtacagtca tccgaacaag tatcggcatc aggtgtgcag tcagacccaa
    11461 ataatccatt tgctcagatt gagcagcagg cagccagtca gcagccacct caacccgtaa
    11521 gttctgtcgc accgaaacct gttcagcaac aaacagttaa tcaggccaat aatgcaccaa
    11581 cacgggaaga accatcattg atgcaacaag ctggcgattg gctcactggt ggtcaaagtg
    11641 cagggcaaat tgcagaacag gctggtcgtg gtctggtaaa cataccattt gacgtattgc
    11701 agggtggcgc aagtctgatt aatgcaatca gtcaggggct tggggggcca aaagtattgg
    11761 atgatgttta tcgtccagta gacagaccga cagaccccta cgcgcaagct ggagagtcaa
    11821 taggcggtta tcttgttcct ggagcaggtg tagctggaaa catggtcatt ggttctctcg
    11881 ctgacgcggc gaatcaacgg ggtgattttg ccgaaaatgc cgctattaat gccggactta
    11941 gcattgctac gcatggcctg ataaatggcg ttacccgtgg tgttcgtggt gcatcaaata
    12001 taattagtgg caataaaaca tctgcacaga gagcgaccac tgcgccaaca gaaacatcac
    12061 cattctccgg tgatgccgct gcagcaacaa atcctgcggt tcatgccgca gaggcaagag
    12121 tagcacaagg tgtaccaatg acgcctgcga cgaagaaccc agaggaagtc gttcgcacag
    12181 tagcagcaca aaaaaggcca aatctcgctt catcgcttga tgaactagat atcaatcctc
    12241 aggctgaagt tctggagtct gctgaaaggc ttaatgttga ttcattactc ccttcacact
    12301 tttccgggaa cgagcaatac aaggcagttg agcaagcaat caagtcccgt gcgggttctg
    12361 ctcta
//
LOCUS       DAJTSI010000115        12219 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000115 DAJTSI010000000
VERSION     DAJTSI010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12219
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.115"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..492)
                     /locus_tag="OO327_002948"
     CDS             complement(<1..492)
                     /locus_tag="OO327_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6019406.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(523..1215)
                     /gene="fimC"
                     /locus_tag="OO327_002949"
     CDS             complement(523..1215)
                     /gene="fimC"
                     /locus_tag="OO327_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR6019407.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1435..1977)
                     /gene="fimA"
                     /locus_tag="OO327_002950"
     CDS             complement(1435..1977)
                     /gene="fimA"
                     /locus_tag="OO327_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR6019408.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIVSSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2458..3324
                     /gene="folD"
                     /locus_tag="OO327_002951"
     CDS             2458..3324
                     /gene="folD"
                     /locus_tag="OO327_002951"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR6019409.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3326..3538
                     /gene="ybcJ"
                     /locus_tag="OO327_002952"
     CDS             3326..3538
                     /gene="ybcJ"
                     /locus_tag="OO327_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR6019410.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3646..4155
                     /locus_tag="OO327_002953"
     CDS             3646..4155
                     /locus_tag="OO327_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6019411.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4191..5576)
                     /gene="cysS"
                     /locus_tag="OO327_002954"
     CDS             complement(4191..5576)
                     /gene="cysS"
                     /locus_tag="OO327_002954"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR6019412.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNSDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5750..6244
                     /gene="ppiB"
                     /locus_tag="OO327_002955"
     CDS             5750..6244
                     /gene="ppiB"
                     /locus_tag="OO327_002955"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR6019413.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6247..6969
                     /gene="lpxH"
                     /locus_tag="OO327_002956"
     CDS             6247..6969
                     /gene="lpxH"
                     /locus_tag="OO327_002956"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR6019414.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7087..7596
                     /gene="purE"
                     /locus_tag="OO327_002957"
     CDS             7087..7596
                     /gene="purE"
                     /locus_tag="OO327_002957"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR6019415.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFNFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7593..8660
                     /gene="purK"
                     /locus_tag="OO327_002958"
     CDS             7593..8660
                     /gene="purK"
                     /locus_tag="OO327_002958"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR6019416.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8797..9690)
                     /locus_tag="OO327_002959"
     CDS             complement(8797..9690)
                     /locus_tag="OO327_002959"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR6019417.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9687..10334)
                     /locus_tag="OO327_002960"
                     /pseudo
     CDS             complement(9687..10334)
                     /locus_tag="OO327_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10337..11569
                     /gene="allC"
                     /locus_tag="OO327_002961"
     CDS             10337..11569
                     /gene="allC"
                     /locus_tag="OO327_002961"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCR6019418.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQE"
     gene            11580..>12219
                     /gene="allE"
                     /locus_tag="OO327_002962"
     CDS             11580..>12219
                     /gene="allE"
                     /locus_tag="OO327_002962"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HCR6019419.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT         2969 a         3158 c         3114 g         2978 t
ORIGIN      
        1 agcgctactc aacaacgcta tctgaggaat agtgatgttc aggcgcatcg ctgcaaaatc
       61 aaagttaatt gtggcatcag gtacagcttt cagtaaattg atgcatttat tgttttgctg
      121 ctgggctaac tcgggaaatg cactgctatt aaggccaatt cgttcaagga gtacctggtt
      181 aaaacagggc attaacccac ctgatttatc acctgtattt tctgttgtcg attcaaatac
      241 gatatcctgt gaaccaatga actcatcatt acgccagaga tccacccgat acacgcccgc
      301 aggttgatga ttacctctct caaagcgcga taaatcagca acggatgtgc cattctctaa
      361 taaaaatgcc gggttgaaat aactttccgc atagcttaaa aaggagggta aaatggcaag
      421 agatgaataa caaattccgg ccagacacca ggtatacctc tgcggaatat ccgtagtagt
      481 gggtattttc ataattagct ctgatattgt cagatgattc ggttagttta agttcacttc
      541 tctgaccggg gttactgaac cataatcatt aacggtctga aatttcagct ttccctgcac
      601 tcctgagggt aagggaattt gtgaactaac tctgggtgcc gccatagcat tccccaactt
      661 ttggctgccc acttgcaggt taacgagggt gacataaaaa ggtgatggat tatgaacatt
      721 gatatagtta ccggatcgcg aaaacttgag tgtgtccggc gcttctgcgg gtaattcttg
      781 taattgaatt ggacggagaa ataatttcat gcgcgataaa atcgccagtt gcaaaacatt
      841 cctgccgttc aatgcatttt tatctaccga agggatcgtc ttaacattca tccagaacag
      901 agactcacga tctgccgcca gcggtggacc ggtgtaaata atacgcaaag tattttcgct
      961 tttgggttca ctaacaaata gtggcggtgt aatgatgaat gacttttctt ttacaccgct
     1021 gctgttttca atccacgaat tgaccagaaa gagctcattg gtatggctat ttttaatcca
     1081 taccgcagtc tgtttagcat cagcgggata aataatacgc gtggcaccta atgcgatccc
     1141 tccggcagca tgggcgctgg ccgaaatgat taaataaaat atcatgagca tcaataactt
     1201 tattttagtc atcatagtta atacgccttg tgtattcctg acggcaaaaa taaggagtat
     1261 aaagaaatga ttgctttggc ttcttacaac acatcattcc cttttcattt aataaagcgg
     1321 catgatacaa agtgaacgtg gtttcgattt acattgccac cataccgctt cagacaaccg
     1381 gtacattact ttgttataag acgtgatata taattcaaaa caacgtggtt ttgattattc
     1441 gtatctcata ataaaagtcg cgtcagcatt tgcttgccct gctgatgcac ttgtaccggt
     1501 gcctttataa cgtgcagaaa aatgaagaac gttggtcccg gggatcaagt tctggttggt
     1561 tgagaagcta ttcccatcag gtttgagaat tgcggatgta ttatcaagta tttcaatacc
     1621 gacacccgtt gctgttgttg tatttgtact ggatacaatg gccaataaat tatcattgat
     1681 tgcatcagcc tgaccagaaa atgcaacggc agcattagcg gcaacaacag gatcgcagtc
     1741 gttcaactga atggtgaatg gaattaatgc agaggtatta ccaacagcat tgaaaatatc
     1801 ggtacgatat tgaccgagtg tgacaacctg gtctgccgag tcagtattca ctgaacaggc
     1861 agcattcacc agttcgcctt caaagtgaat tgtaccgcca tctacaacgg cagcataact
     1921 acccgtagcg gcgaatagtg cggcagccag cgcagacgaa ataaatctta atttcatata
     1981 tattccttca atctcattta tcaactccac atccgtatat aaccgattac tttatttaag
     2041 acactgatag tagtaaaatt cctttttatc ctctaagaat gtcttaattg aaaatatgca
     2101 ctctattcta aaaaatagag agccccgtta gatgaatact tccgcgcaaa atatattcac
     2161 cacaaatata gacctgaagc ggtaaattac caggctgaaa attcttttta tattgtcagg
     2221 tatttcttaa attatcttaa tccttaaaca aggaaataaa tcagttccag atttacaacg
     2281 ccatcatgta cgaaaaatga agctttcaat ctcagcgacc tcagcgacgg tgcgcctaac
     2341 cttcgcaaga ggtcgcttca cgcgataaat ctgaaacgaa acctgacagc gcgccccgct
     2401 tctgacaaaa tagacgcatc cccttcgacc tacgtaacag atggaatcct ctctctgatg
     2461 gcagcaaaga ttattgacgg taaaacgatt gcgcagcagg tgcgctctga agttgctcaa
     2521 aaagttcagg ctcgtattgc agccggactg cgggcaccag gactggccgt tgtgctggtg
     2581 ggtagtaacc ctgcatcgca aatttatgtc gcaagcaaac gcaaggcttg tgaagaagtc
     2641 gggttcgtct cccgctctta tgacctcccg gaaaccacca gcgaagcgga gctgctggag
     2701 cttatcgatg cgctgaatgc cgacaacacc atcgatggca ttctggttca actgccgtta
     2761 ccggcgggta ttgataacgt caaagtgctg gaacgtattc atccggacaa agacgtggac
     2821 ggtttccatc cttacaacgt cggtcgtctg tgccagcgcg cgccgcgtct gcgtccctgc
     2881 accccgcgcg gtatcgtcac gctgcttgag cgttacaaca ttgatacctt cggcctcaac
     2941 gccgtggtga ttggcgcatc gaatatcgtc ggtcgcccaa tgagcatgga actgttgctc
     3001 gcaggttgca ccaccaccgt gactcaccgc ttcactaaaa atctgcgtca tcacgtagaa
     3061 aatgccgatc tgttgatcgt tgccgttggc aagccaggct ttattcccgg tgactggatc
     3121 aaagaaggcg caattgtgat tgatgtcggc atcaaccgtc tggaaaatgg caaagttgtg
     3181 ggcgacgtcg tgtttgaaga cgcggctaaa cgcgcctcat acattacgcc tgttcccggc
     3241 ggcgttggcc cgatgacggt tgccacgctg attgaaaaca cgctacaggc gtgcgttgaa
     3301 tatcatgatc cacagggtga gtaacatggc gacattttct ttaggtaaac atccgcacgt
     3361 tgagctgtgc gacttgctga aactggaagg ctggagcgaa agcggcgcgc aggcgaaaat
     3421 cgcgattgcc gaaggccagg tgaaagtcga cggtgcggtt gaaacgcgca aacgctgcaa
     3481 aatcgtcgcc ggtcagacag tgagttttgc aggacacagc gtacaggttg ttgcctgatc
     3541 cctttcccgg cctgtatcaa caggccggtt ctcttttcag acttttgccc tctacgcccc
     3601 gttcatccat gctgatagca tcgacatttt gccgaagcga ccatcatgcc aaccgttatt
     3661 acgcacgccg ccgttcccct ttgcattggt ttagggttgg gttcgaaagg tctgttattt
     3721 gccggaatca tcctggcgat gctgcccgat gccgacgtat tgtcgtttaa atttggcgtt
     3781 gcttacggca atgtttttgg tcatcgcggt tttacccatt cgctggtgtt tgcgtttgtt
     3841 gtcccgctct tatgtgtgct gattggacgt cgatggttca gggctgggct gattcgctgc
     3901 tggctgtttt taaccgtctc gttgctatcg cacagcttac tggattcggt aaccactggc
     3961 ggtaagggcg ttggctggct gtggccgtgg tcagatgaac gctttttcgc tccctggcag
     4021 gtgattaaag tcgcgccgtt tgcattgtct cgttacacca cagcgtacgg gcatcaggtg
     4081 attatttccg aattgatgtg ggtgtggttg ccggggatgc tactgatggg aatgttgtgg
     4141 tggcgcagac gataaccgga tgcgaaaact cgcatccggc aattgcgcaa ttacttacga
     4201 cgccaggtgg tcccttgcgg gccatcttcc agcacgatcc ccatctcgtt aagacggtca
     4261 cgtgccgcat ctgccgccgc ccagtctttc gctttacggg catccagacg ctgttgaatt
     4321 aacgcttcaa tctcagccac ttcgctgtcg tctgcctgcg cgccgctttg caggaacgct
     4381 tccggttctt gctccagcag gcccaataca gcggaaagtt tacgcaggtg agatgccatt
     4441 gcattcgccg ctgccatatc ttctgctttc agacggttca cttcacgcgc catatcaaac
     4501 agcacggaat aggcttccgg ggtgttgaaa tcgtcgtcca tcgcttcaat aaagcgcgct
     4561 tcaaacgctt cgccaccggc aggcgcaacg gttttatctg tgccgcgcag cgcagtgtag
     4621 aggcgctcca gcgccgcacg cgcctgcttc aggttctctt cgctgtagtt cagctggctg
     4681 cgatagtggc ccgacatcag gaagtaacgc acggtttccg cgtcgtagta tttcagcaca
     4741 tcgcgcacgg taaagaagtt acccagcgat ttggacatct tctcgcggtc aaccatcacc
     4801 attccggagt gcatccagta gttcacatac tgaccatcat gggcacaggt ggactgcgcg
     4861 atttcgttct cgtggtgcgg gaacatcagg tctgaaccgc cgccgtggat atcaaagtgg
     4921 ttacccagct gcttgcagtt catggccgaa cattcaatgt gccagccagg acgacccgcg
     4981 ccccacggag acggccagct cggttcgccc tctttcgaca tcttccacag aacgaagtcc
     5041 atccggttgc gtttgtcatc aaccacgtca acgcgcgcgc cagcctgcag ctggtcgaga
     5101 tcctgacgcg acagcacgcc ataagttgga tcggtcggga cgtcgaacat cacgtcgctg
     5161 ttgtccgcca cataagcgtg acctttggcg atcagttgtt cagtgagttc aataatttct
     5221 gcgatatggt gcgtcgcgcg cggctccata tccgggcgca gaatgttcaa agcatcaaaa
     5281 tctttgtgca tttcggcgat catgcgatcc accagcgcca caaagctttc gccattttca
     5341 ttggcgcgtt tgatgatttt gtcgtcgata tcggtaatgt tgcgcacata cttcagttta
     5401 tagccgagga aacgcagata gcgcgcaacc acgtcaaaag caacaaaggt acgcccgtga
     5461 ccgatatgac agagatcgta aacggtgatt ccacacacgt acatgccgac ttccccggcg
     5521 tgaataggct taaattcctc tttttggcgt gtcagagtat tgaagatttt tagcatcgaa
     5581 gattccgttt agacatgtgt gggtaattga gttgcgtata atacccatat ttcccgcgcg
     5641 aatcagcata cattgcgtga tgatccgatc gtgcggttat gctataacac caccctatat
     5701 atgacccgaa ctgggttgaa gcaccaatca aacggaacag gatgcaaaaa tggttacttt
     5761 ccacaccaat cacggcgata ttgtcatcaa aacttttgac gataaagcac ctgaaacagt
     5821 taaaaacttc ctggactact gccgcgaagg tttttacaac aacaccattt tccaccgtgt
     5881 tatcaacggc tttatgattc agggcggcgg ttttgaaccg ggcatgaaac aaaaagccac
     5941 caaagaaccg atcaaaaacg aagccaacaa cggcctgaaa aatacccgtg gtacgctggc
     6001 aatggcacgt actcaggctc cgcactctgc aactgcacag ttcttcatca acgtggttga
     6061 taacgacttc ctgaacttct ctggcgaaag cctgcaaggt tggggctact gcgtgtttgc
     6121 tgaagtggtt gacggcatgg acgtggtaga caaaatcaaa ggtgttgcaa ccggtcgtaa
     6181 cggtatgcac caggacgtgc caaaagaaga cgttatcatt gaaagcgtga ccgttagcga
     6241 gtaatcgtgg cgacactctt tattgcagat cttcatctct gcgtggaaga accggcgatc
     6301 accgccggtt ttctgcgttt tttagcgggg gaagcccgca aggccgacgc gctgtatatt
     6361 cttggcgatc tgtttgaagc atggattggc gacgacgatc ccaacccact ccatcgccag
     6421 atggcggcag cgatcaaagc ggtgtccgat tccggcgttc cctgttattt cattcatggc
     6481 aaccgtgatt ttctgctcgg caaacgcttt gcccgtgaaa gcggcatgac attattgccg
     6541 gaagaaaagg tgctcgaact ttatggtcgc cgggtgttga ttatgcatgg cgacacgctg
     6601 tgcaccgatg acgcgggtta tcaggctttt cgcgccaaag tccacaaacc ctggctgcaa
     6661 atgctattcc tcgccctgcc gttgtttgtg cgcaaacgca ttgccgcgcg aatgcgcgcg
     6721 aacagcaaag aagccaacag cagtaaatcg ctggcgatca tggacgttaa ccaaaacgct
     6781 gtggtcagtg cgatggaaaa acatcaggtg caatggctaa tccacgggca tacccatcgc
     6841 ccggcggtgc atgaacttat cgccaaccag caacctgctt ttcgcgtggt actgggtgcc
     6901 tggcatacgg aaggttcgat ggtgaaagtc acggctgatg acgttgagct gattcatttt
     6961 ccgttttaaa aaacccacaa ctttgctgat ttcacagcca cgcaaccgtt ttccttgctc
     7021 tctttccgtg ctattctctg tgccctctaa agccgagagt tgtgcaccac aggagtttta
     7081 agacgcatgt cttcccgcaa taatccggcg cgtgtcgcca tcgtgatggg gtccaaaagc
     7141 gactgggcta ccatgcagtt cgccgtcgaa atcttcgaaa tcctgaatgt cccgcaccac
     7201 gttgaagtgg tttctgctca ccgcaccccc gataaactgt tcaacttcgc cgaaagcgcc
     7261 gaagagaacg gttatcaggt gattattgcg ggcgcaggcg gcgcagcgca cctgccaggc
     7321 atgattgccg ccaaaacgct ggtgccggtg ctgggcgtgc cagtacagag cgccgcactg
     7381 agcggtgtcg atagcctcta ctccatcgta caaatgccgc gcggcattcc ggtgggtacg
     7441 ctggcgattg gtaaagctgg cgcggcaaac gcggcattac tggcagcaca aattctcgcg
     7501 actcacgata aagagctaca ccagcgtctg aatggctggc gcaaagccca gaccgacgaa
     7561 gtgctggaaa acccggaccc gcgaggtgcg gcatgaaaca ggtttgcgtc ctcggtaacg
     7621 ggcagttagg ccgtatgctg cgtcaggcag gcgaaccgtt aggcattgct gtctggccgg
     7681 tcgggctgga cgctgaaccg gcggcggtgc cttttcaaca aagcgtgatt accgctgaga
     7741 tcgaacgctg gccggaaacc gcattaaccc gcgagctggc gcgtcatccg gcctttgtga
     7801 accgcgatgt gttcccgatt attgctgacc gtctgactca gaaacagctt ttcgataagc
     7861 tccacctgcc gaccgcgccg tggcagttac ttgccgatcg cagcgagtgg cctgcggtgt
     7921 ttgatcgttt aggtgaactg gcgattgtta agcgtcgcac tggtggctat gacggtcgcg
     7981 gtcaatggag tttacgcgcc aatgaaaccg aacagttacc ggcagagtgt tacggtgaat
     8041 gtattgtcga gcagggcatt aacttctctg gtgaagtatc gctggttggc gcgcgcggct
     8101 ttgatggcag caccgtgttt tatccgctga cgcataacct gcatcaggac ggtattttgc
     8161 gcaccagcgt cgcttttccg caggccaacg cacagcagca ggcgcaagcc gaagagatgc
     8221 tgtcggcgat tatgcaggag ctgggctatg tgggcgtgat ggcgatggag tgttttgtca
     8281 ccccgcaagg tctgctgatc aacgaactgg ctccgcgtgt gcataacagc ggtcactgga
     8341 cacaaaacgg tgccagcatc agccagtttg agctgcatct gcgggcgatt accgatctgc
     8401 cgttaccgca accagtagtg aataatccgt cggtgatgat caatctgatt ggtagcgatg
     8461 tgaattatga ctggctgaaa ttgccgctgg tgcatctgca ctggtacgac aaagaagtcc
     8521 gtccggggcg taaagtgggg catctgaatt tgaccgacag cgacacatcg cgtctgactg
     8581 cgacgctgga agccttaatc ccgctgctgc cgccagagta tgccagcggc gtgatttggg
     8641 cgcagagtaa gttcagttaa gcaggtgctc atttgattgc cggatgcggc gtaaacgcct
     8701 tatccggctt accgctccag tacccattgt aggcctgata agacgcgtca agcgtcgcat
     8761 caggcatggt gcaccgattg ccggatgctc tcatcactac agcgaaatac aggtccccgc
     8821 ttcgcccgcc agcgtctctt caattcgcga taacgcccca atccacgcgg gtttactacg
     8881 gcttctgaca taaccactca ccgccgttac cttcggcccc atcgaaccat cggctttggc
     8941 aaatggcgct aactcatccg gtgtggcatg gcgaatggca cgttgctgcg gcgttcccca
     9001 gttttcatat accgcatcag catcggtgag gatcaccagt ccatcggcat taatctgctc
     9061 ggcgagcaac gcagcggcga gatctttatc aatcaccgct tcactccctg ccccatcttc
     9121 cgtcacaggc acaccgccgc cgccactgca aatcaccaca tgcccctctt tgagcaacaa
     9181 ctcgatggct tcgctgtcga gaattttacg cggttgcgga gacgccacca cccggcgcaa
     9241 atatttacca tcacgtttca tctgccagcc gtaagccgct tccagtgcct cttgttcttc
     9301 tggctgataa accggaccaa taaatttctc tggctgcaaa aacgccggat catcaggcga
     9361 aacctcaatg cgcgtcagca ccgtcgtaac aggcggcatc tgcggctgtg cgctcaagct
     9421 ctgcgccagc atatagccaa tcatcccctg gctttccgcc accagtacat ccagcggata
     9481 cggttctacc tctttccacg ccagattctg taacgccagc agccccacct gcggcccgtt
     9541 gccgtgaaca atcgccagcc gataagaacg ggccaggcgt gccagcgcgg gtacagcact
     9601 ggcgatattg cgatattgat tttccgccgt cagcgcctca ccgcgctgga gtaaggcgtt
     9661 gcccccaaga gctacaacca gtgttttcat ggttttcctt gtaataattg ttggccaagc
     9721 cagaacccca gcagcgtatc tgcccctgac gtatgcccca gcgccagcag cgaatcaatc
     9781 gcaacagcgg tacgtttcgg gcaactcaga gcatggacaa agtgcaggag tggcgaggcg
     9841 aaatatcctt gcgcggcata acgtaaataa ctgacgctca ccgctgtggt aacgagttga
     9901 agattgtcgg aacaggcaaa aaacgggcga ccggcgcgcg catctaaagc gccataatac
     9961 caggccgcca gcagcattcc gctcaacgtg tcatcatgac ttggcgttaa tccggggcct
    10021 ttacccagcc agtgccgcca gtcggtctta acgccattga gcgcggcctg aaaacagtga
    10081 cgaaactggc gtaattcagc gggcagcgga tcgcttgccg ccatcgccag tggcccgaaa
    10141 agtccggttt cctccgcgcg ttgcatccat gcagctgcaa gcggttgagg atgcgaaggc
    10201 ggcgtaatac gcagcaaaca ataacgctgt ggctgtttaa ccgtgaaacg cccgaggcga
    10261 atcccttgag ccacaacctg tggtcgttca ttgccgcata atccaccgca taacgcatcg
    10321 aattgcgtgc ggcgaaatta cacattcccg tcaagctata gaagaaacgc tgccctggct
    10381 ttcctctttt ggcgctgacc caacgggtgg gatgacccgt ttactttatt cgccggaatg
    10441 gctggaaacc cagcagcaat ttaaaaaaag aatggcagca agtgggctgg aaacacgttt
    10501 cgatgaagtg gggaatttat acggtcgcct gagtggcacc gaatatccac aggaagtggt
    10561 tctgagcggt tcgcatatcg ataccgtggt taacggcggt aacctcgacg ggcaattcgg
    10621 cgcgctggcg gcgtggctgg caattgactg gttgaaaacg caatacggcg caccgttacg
    10681 tacggtcgaa gtggtggcga tggcagaaga agaaggcagc cgcttcccgt atgtcttctg
    10741 gggcagtaaa aatatctttg ggctggcgaa tcctgacgac gtgcggaata tctgtgatgc
    10801 caaaggaaat agttttgccg atgcgatgaa ggcttgcgga tttactctgc cgaacgcccc
    10861 actaactccg cgtcaggcta taaaagcctt tgttgaactg catattgaac agggctgtgt
    10921 gctggaaagt aatgggcaat caattggcgt ggtgaatgca attgtcgggc agcgtcgcta
    10981 tacggtgacg ctgaacggcg aatcaaacca tgcaggcacc acgccgatgg gttatcgtcg
    11041 tgatacggtt tacgctttca gtcgcatttg ccatcagtcg atcgaaaaag cgaaaaagat
    11101 gggcgatccg ctggttctga cctttggcaa agtagagccg cgcccgaata cggtaaatgt
    11161 ggtgccgggt aaaaccacgt tcaccattga ttgtcgtcat accgacgctg ccgtgctgcg
    11221 cgatttcacc caacagttag aaaacgacat gcgggcgatt tgcgatgaaa tggacattgg
    11281 tattgatatc gatttatgga tggacgaaga acccgtgccg atgaataagg agctggtcgc
    11341 caccctgaca gaattgtgtg aaagagaaaa actgaattac cgggtgatgc acagtggtgc
    11401 cgggcacgac gcgcaaattt tcgcgcctcg cgtaccaacc tgcatgattt ttatccccag
    11461 catcaacggg atcagccata acccggcgga acgcaccaat attactgacc ttgccgaagg
    11521 ggtcaaaacg ttggcactca tgctttatca acttgcctgg caggaataag gagtcataaa
    11581 tgggatattt aaataacgtc accggttacc gcgaagattt actggctaac cgtgcgattg
    11641 ttaaacacgg taatttcgca ctgttaaccc cggatggtct gataaaaaat attattccgg
    11701 actttgaaaa ttgtgacgcg acaatcctct ccacgccaaa gctgggtgcc tcttttgttg
    11761 attatctggt cacactgcat caaaacggtg gcaaccaaca gggcttcggt ggcgaaggca
    11821 ttgaaacgtt cctgtatgtg atctctggaa atatcactgc caaagccgaa ggcaaaacat
    11881 ttgccttaag cgaaggtggc tatctttatt gcccaccagg ctccttaatg acgtttgtta
    11941 acgcccaggc cgaagacagc caaatctttt tatataagcg ccgctatgtt ccggtagaag
    12001 gccatgcacc gtggctgttt tctggcaatg ccagcgaact ggaacgcatt cactatgaag
    12061 gtatggatga tgtgatcctg ctggattttc tgcccaaaga gttaggtttt gatatgaaca
    12121 tgcatatcct ctcttttgca ccaggtgcca gccacggtta tatcgaaaca cacgttcagg
    12181 aacatggtgc ctatattctt tccggtcagg gggtttata
//
LOCUS       DAJTSI010000116        12184 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000116 DAJTSI010000000
VERSION     DAJTSI010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.116"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..602)
                     /locus_tag="OO327_002963"
     CDS             complement(<1..602)
                     /locus_tag="OO327_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR6019420.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            1122..2021
                     /locus_tag="OO327_002964"
     CDS             1122..2021
                     /locus_tag="OO327_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6019421.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            2070..3083
                     /gene="yhjD"
                     /locus_tag="OO327_002965"
     CDS             2070..3083
                     /gene="yhjD"
                     /locus_tag="OO327_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR6019422.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            3494..4816
                     /locus_tag="OO327_002966"
     CDS             3494..4816
                     /locus_tag="OO327_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR6019423.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(4998..7058)
                     /locus_tag="OO327_002967"
     CDS             complement(4998..7058)
                     /locus_tag="OO327_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6019424.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(7128..7895)
                     /gene="pdeH"
                     /locus_tag="OO327_002968"
     CDS             complement(7128..7895)
                     /gene="pdeH"
                     /locus_tag="OO327_002968"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR6019425.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTSASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            8127..9056
                     /gene="kdgK"
                     /locus_tag="OO327_002969"
     CDS             8127..9056
                     /gene="kdgK"
                     /locus_tag="OO327_002969"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR6019426.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(9152..10648)
                     /locus_tag="OO327_002970"
     CDS             complement(9152..10648)
                     /locus_tag="OO327_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6019427.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTNIPPAQ"
     gene            complement(10869..12155)
                     /gene="dctA"
                     /locus_tag="OO327_002971"
     CDS             complement(10869..12155)
                     /gene="dctA"
                     /locus_tag="OO327_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR6019428.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
BASE COUNT         2919 a         3309 c         3001 g         2955 t
ORIGIN      
        1 caggaggaga tatttaacat cattgccgcc tgggtgcgat ttttcacttc cagacggcga
       61 tacagggatt ccagatgcgc ttttaccgtt ccggtactga tattcagcgc tctgccgatc
      121 tccttatttg attcgcccgc cgctaacatg gttaaaatct cccgctggcg ggcgcttaac
      181 gatttgagat ctttaatgtc cttttccggc gtcgtccgcc agtctccagg cagaaacatc
      241 atccccatcg ccgcactatt taccgccaac gcaaatgtct cgacggttga atcgcgaggc
      301 acaatggcca gcacattaaa atggataact tcctgtaacc accgtttatt gcaatccgtc
      361 gccgtgatta acaccttaac ctcaggaaac tgcaccacgg ttttttgcag caaccagtag
      421 caaaactcac catcctgatc gccatcgagc ataactaagg cttcagggta actttccagc
      481 ttttgccata actcgtctgc ctgactgacc ccctgaatac tcactcctgg aatacgctgc
      541 tgtaaactga tttttattcc atgaataaat attgactgcc tgtcaaacat gactatttgc
      601 ataactgaat ctccacctga atacgttaaa aagacttaag tagtggaagg gtattacccg
      661 cgagaaaaaa taagaattcg ccatttggcg gtggccattc tacagagatg acgcgtagaa
      721 aatagttacc gatataaata gttacagcta aacgcctgaa attacatgtc gagggcacta
      781 tttaaaacaa ttttgaggat ttccttatat tggtggttag tacgcatgca attaaaaatg
      841 aaattccgcg actacaagcc aaaataacaa atggcaatga gacaaaaata agcacaaata
      901 gccaatacgt cctctattca ctttaaaggg aatcactgaa aaatacgctc tgtttaaggg
      961 gattcacctt tctcataaag ctattccgcc cttttcctgc tgagaaatcg ccacattcgt
     1021 catgacaaca ttgtgaaacc cggcattaga tgttagaaaa aacaataaac aatgcgatat
     1081 gcgcgttact gtttcgctgt gaacaacaat cggtcaaaga aatggataaa attcacgcaa
     1141 tgcagttgtt catcaaagtc gcggagctgg aaagtttttc ccgcgcggcg gatttctttg
     1201 ctttgccaaa gggaagtgtt tcgcgccaga tacaggcact ggaacatcaa cttggcaccc
     1261 agcttctcca gcgcactacg cgacgggtca aactcacgcc agaaggcatg acctattatc
     1321 aacgggcaaa agatgtgttg agtaatctca gcgaactgga cggtctgttt caacaggatg
     1381 ccaccagtat cagcggtaaa ttacgcatcg acatcccgtc aggaatcgcg aaaaacctgt
     1441 tactgccgcg tctgtcggaa tttctctatc agcatccggg aattgagctg gaactgagta
     1501 gccataaccg tccggtagat attcttcatg atggttttga ttgcgtgata cgcactggcg
     1561 cgttaccgga agatggcgtt atcgcccgtc ccctcggcaa actaaccatg gtcaactgtg
     1621 ccagtccgca ctatctgacg cgctttggtt atccgcaaag ccccgatgat ctgacttcac
     1681 acgcaatagt gcgttataca ccgcacctgg gtgtacatcc gttaggtttt gaggttgcca
     1741 gcgttaatgg cgtccagtgg tttaagtctg gcggcatgtt gacggtaaac agtagcgaaa
     1801 actatctcgc cgccggtctt gccggtctgg ggattattca gatcccgcgc attgccgtgc
     1861 gcgaagccct gcgtgccggg cggcttattg aagtgttgcc aggctaccgt gccgagccgc
     1921 tctccctttc gctggtttac ccgcagcgtc gggagctttc ccggcgtgta aacctgttta
     1981 tgcagtggct ggctggcgta atgaaagagc acctggactg accgactata ctttttaaga
     2041 atgaccacga cagaaggaca aaagagcgga tgacgcagga aaacgagatc aaacgtccca
     2101 cccaggatct ggagcacgag ccgattaagc agttggataa tagcgagaaa ggcggcaaag
     2161 ttagccaggc gctggaaacc gtcaccacca ctgccgaaaa agtccaacgc caaccggtta
     2221 ttgcgcacct tattcgcgcg acagaacgct ttaacgatcg gctgggtaac cagtttggtg
     2281 cggctatcac ctatttctca tttttgtcga tgatcccgat tttgatggtg tcgtttgccg
     2341 cagggggctt tgtgctggcc tcccatccga tgttgctaca ggatatcttc gacaaaattc
     2401 tgcaaaacat cagcgatccg acactagccg ccacgttgaa aaacaccatc aacaccgccg
     2461 ttcagcagcg tacgactgta gggcttgtcg gcctggcggt ggcactttat tccggcatca
     2521 actggatggg taacctgcgt gaagcgattc gtgcccagtc gcgcgatgtc tgggaacgct
     2581 cgccgcaaga tcaggagaag ttctgggtaa aatatctgcg tgattttatt tcactgattg
     2641 gtttgttgat tgcgctgatt gtgacgcttt cgatcacctc ggttgccggt tcggcgcagc
     2701 aaatgattat tagcgccctg cacctgaaca gcattgagtg gctgaaaccg acgtggcgat
     2761 tgattggcct ggcgatttcc atcttcgcca actatctgct tttcttctgg atcttctggc
     2821 ggttgccgcg ccaccgtccg cgcaaaaaag ccctgattcg cggaacattt ctggctgcta
     2881 ttggttttga agtgattaaa atcgtgatga cctacaccct gccatcgctg atgaaatccc
     2941 cctctggcgc agcatttggt tccgtgctgg ggctgatggc atttttctac ttcttcgccc
     3001 gtttgacgct gttttgcgcg gcgtggattg ccaccgccga atataaagac gacccgcgaa
     3061 tgccagggaa aacgcagcct taaaataggc cggatgcggc gtaaacgcct tatccggcat
     3121 acgattttat tcggcctggc tccccgtagg ccggataaga tgcgccagca tcgcatccgg
     3181 ctataatgcg cacataacct cttgaaactc ttccccagag cctcttcagc catctatttg
     3241 ggagcaaaca atttcactcc aactcataac cccagcatat aaatccagtt ggtaactttt
     3301 atttaacctg aaaccagttt tatccactat ttataaaatt atgtgaagca tttcatagaa
     3361 gaaaaatcac tggcctaaac attatcccct ttttgcctgg tttttgacca tttccgcgat
     3421 ttgttacaca ttgaaatatc acttttgctg tgcgtaatat ggctattcgt tagccaaaaa
     3481 ataagaaaag attatgcaag caacagccac aacactcgac cacgaacaag aatacacgcc
     3541 gatcaactcg cgtaataaag tccttgtcgc ctctctcatt ggcacggcca ttgagttctt
     3601 cgacttttac atttacgcca ctgcggccgt tattgtgttt ccgcatatct tcttcccgca
     3661 gggcgatcca acggcagcaa cgctacagtc gctcgccacc ttcgccatcg ccttcgtcgc
     3721 gcgccccatt ggctctgccg tttttggtca ttttggcgat cgcgttgggc gtaaagcgac
     3781 gctggtcgcc tcgttgctaa cgatggggat ttcgaccgtg gtgattggtc tgctgccggg
     3841 ctatgccacg attggtattt tcgccccact gctgctggcg ctggctcgat ttggtcaggg
     3901 tctgggctta ggcggtgaat ggggcggcgc ggcgctgctg gcgactgaaa acgccccacc
     3961 gcgcaaacgt gcactgtatg gctccttccc acagctaggc gcaccgattg gcttcttctt
     4021 tgccaacggc actttcttgc tgctttcctg gctactgacc gatgagcagt ttatgagctg
     4081 gggctggcgt gtgccattta tcttctcggc ggtgctggtc attatcggcc tgtatgttcg
     4141 cgtgtcgctg catgagtcgc cggtgtttga gaaagtcgct aaagcgaaaa aacaggtgaa
     4201 gatcccgctg ggtacgctgc tgaccaaaca tgttcgcgta accgtactgg gtacgttcat
     4261 tatgctggca acctatacgc tgttttacat catgacggtc tactctatga ccttcagtac
     4321 cgccgccgcg ccagttgggc ttggcctgcc gcgtaacgaa gtgttgtgga tgctgatgat
     4381 ggcagttatt ggttttggcg tgatggtgcc agtcgccgga ttactggctg atgcctttgg
     4441 tcgccgtaaa agcatggtga tcatcaccac gctgatcatc ctgttcgcgc tgttcgcctt
     4501 taacccgctg ctcggttctg gcaacccgat tctggttttt gccttcctgc tgctggggtt
     4561 aagtctgatg gggctgacct tcgggccgat gggtgcgctg ttaccagagc tgtttccgac
     4621 agaagtgcgt tacaccggag catcgttctc ttacaacgta tcgtcgattc tcggggcttc
     4681 cgttgcgccg tatatcgcag cctggttgca ggctaactac gggttaggtg cggtggggtt
     4741 atatctggcg gcgatggctg gcttgacgtt aatcgccctg ctgctgaccc atgagacgcg
     4801 acatcagtcg ttgtaataag tgtgaaaagc ctgatgcgct acgcttatca ggactgaatt
     4861 tccatacaat atattgaatt cacaaggatt tgtaggccgg ataaggcgtt cacgccgcat
     4921 ccggcatgaa caaagcacac tttgccaaaa atctaaatct gtttaattgc cggatgtcag
     4981 acatccggca attatcatca cttcttcatc tgcgacaata tcgtccgaca ctgattagcc
     5041 tccccttcgg aaggtgagat cagtgccagt aacgccgccg ccggtgttac cagcgttgcc
     5101 agtgccgcag caacagcacc acgggcaatc agcggtccgg ctttcacccc agcctgcgga
     5161 tttttaaacg tcccacgcac atacagcggc gaacgcagtg tgataatccg aatcccttta
     5221 ctctccggat caatagtcaa atccagctgt tccgaagcaa aacttgccgt gccggtaaca
     5281 ttaatcaacg cgttctcagt atcgaaagca aaaatctgcg ggcgcgccac gccgttggca
     5341 atattcagat tcgccgccgc gcagttcacc cgcacctcat cgtcaccaaa tatcgcaccg
     5401 acaatgtagt tgccgacatt cagcccaaca atctccatca ggttgcggct caccagcccg
     5461 tcattcatca acagtttcag gttgccgtta ctgttgccta aaagcgccgc caccgagtta
     5521 ccgctaccgc gtagttccgc gtcaccgttc atttccccca gcgtcttctg catcagttcc
     5581 acatcgggca tcagttcttt cagtttcagt cgacgagcct gaatatctgc ccgcccctgc
     5641 atcggctttt tatcgccttc cagatgaata ttcgccgcaa tgctgccgcc cgccatgcca
     5701 aatttcagcg gttgcaggcg caggtcagca tttttgagga tgatatgagt agaaagatcg
     5761 ctaatcggca ggctactgcc atgctcaatg cgccgccctt tgaagcgaac atcggcatcc
     5821 ataacgtccc atttatcggt ttcgaagcgg tcataaggca gcactttgcc cgcaggctga
     5881 acgctttttt cgcccttctt ctgttcagac cgtttcgact tttctgcccc tttcccggaa
     5941 tcaacgccaa tcaacggtcc caggtccgcc agccgcaatt gccgcgactc gacatcacct
     6001 tccagttttg gtcgtggctt tccggtggtg tagaccagag aaccgtggat atcgctatcg
     6061 ccaattcgcc cattaaaacc gcgataatca aagaccgacg atttttcagt gtcgattttc
     6121 gctaccagcc gaccatccgt ttcaaacggc ggggtatcgg gcagcagaac gcccgtcagt
     6181 tcatagagat cacccagtga atcgccagaa aatttaagcc gtaaatcgac accgcccatc
     6241 ttcattgggt cattcacgac gccatcaaaa gcaacacggg tattacctga acggaaatca
     6301 gcctgtaccg gaaacggcgt cccttcgcca cgcagcgcca gcataccgcc tatttttccc
     6361 gtacccgtga gcggttcacc gttataacgt ccctgcgcct tcaggccaaa aatgtaatcg
     6421 cccacctttt ctttatccgc tttacctttc gatccagtaa cttcgctgaa tggcagcggc
     6481 ttgcctaacg gatcaacaaa aatttccaga tccgctttgc ttactttgtc atcaatggcg
     6541 atccgccctt gatcgaaaag aatattatcc agccgaaacg accatgccga cggctttgca
     6601 ttcgcgtctt tgttgtcatc gttggcaaga ttaaacgtcc agttattgtt cttttcagag
     6661 aggcgaatca ggcgcgcgtc gggcttttcg agcttgatcc acggcagcca gaccgttttg
     6721 gtcagcagcg ccagcggtgc cagcgttgcc tctactcgcg gcaaatgcac catcgtgact
     6781 tcgggaatat ccggtggatt accaagaatg atgtcttctg catgtacatg aggccacggc
     6841 acccagctgc gccagccagt ttcttgtttt tgccgctccc acaccacgcc cagattgcca
     6901 cggatagcga acggacgatt caactccgca gagactttct ggttgatggt cggtttgagt
     6961 cgattccagt caaaagttgc aatcaaaatg atcgccacga caatcaacaa caagaaagcc
     7021 cctgaaatcg cagcggttat tttgcctgcc ttgctcatcg ttcgcttcct catattcttc
     7081 ctgtgccagt cctaaagata gtccagcccg gcggaaaatg aggcagctta tagcgccaga
     7141 accgccgtat tcagcgtttc catcggtgcc gggcgtgaaa gaaaccagcc ttgtgcggcg
     7201 aatgcgggcg agttctgaac atcacgccac tcttccggtg tttctacgcc ctcgacaatc
     7261 accccgcgac aatagcgatt cattagatgt aaaagctgag aaaagagtgt gcggccttcc
     7321 ggcgactgac gcagcatcac aaacagttct cgcgcgattt tgatgtagtc ataacgcact
     7381 tcacttagtg cagagaaatt tgccatcccg gtaccaaaat catccagcca cagcgggcca
     7441 aattcacaca tcgaggcaga ggttgaatct ttcggcagac ggatatgctc caccagttcg
     7501 aaacgcagcc agggaagacg ctcaatctgg cgcaggattt ttggttgctg acgcagggcg
     7561 atgagcgtag ggccatcaat attgaccgat gccagcaggc cgtgctctac aaagaagtcg
     7621 gctttttgcg ccagcaaatc aatctgctct ttcacaacct ccatccgatg gctgacggtg
     7681 atttcagtaa aatagcgatc cggcggcagg cgttgcgaag ggttcaaggg atgcgtgacc
     7741 accgttaata gctccacggc cattaaccgc ccgcatgttt gatagatcgg ctgccaggtg
     7801 taagcacgct cacactgcaa ccaaaaacgc cgttcctgca agctctcgat gcttgcttca
     7861 gggttgctta ttcgctggat aacctgcctt atcatcaaag atgtcctgtg tttaaaagtg
     7921 atgctgcccg gactcgacaa agattatcgg cgcgtaaggg cagaacttta ccgcctggcc
     7981 ggggtaaatt tcctcactcc acaacggaat ctttcaggaa tacgttctgg ctcaaaaaaa
     8041 taatggaaca ctgttttaat atggttgacc agcaaaccac cacagcgcaa actaacgcta
     8101 attttttaca gatcaggttc acgactatgt ccaaaaagat tgccgtgatt ggcgaatgca
     8161 tgattgagct ttccgagaaa ggcgcggacg ttaagcgcgg tttcggcggc gataccctga
     8221 acacttccgt ctatatcgcc cgtcaggtcg atcctgcggc attaaccgtt cattacgtaa
     8281 cggcgctggg aacggacagt tttagccagc agatgctgga cgcctggcac ggcgagaacg
     8341 ttgatacttc cctgacccaa cggatggaaa accgtcttcc gggcctttac tacattgaaa
     8401 ccgacagcac cggcgaacgt acgttctact actggcgtaa cgaagccgcc gccaaattct
     8461 ggctggcgag cgagcagtct gcggcgattt gcgaagagct ggcgaatttc gattatctct
     8521 acctgagcgg gattagcctg gcgatcttaa gcccgaccag ccgcgaaaag ctgctttccc
     8581 tgctgcgcga atgccgcgcc aaaggcggaa aagtgatttt cgacaataac tatcgtccgc
     8641 gcctgtgggc cagcaaagaa gagacacagc aggtgtacca gcaaatgctg gaatgcacgg
     8701 atatcgcctt cctgacgctg gacgacgaag acgcgctgtg gggtcaacag ccggtggaag
     8761 acgtcattgc gcgcacccat aacgcgggcg tgaaagaagt ggtggtgaaa cgcggggcgg
     8821 attcttgcct ggtgtccatt gctggtgaag cgttagttga tgttccggcg gtgaaactgc
     8881 cgaaagaaaa agtgatcgat accaccgcag ctggcgactc tttcagcgcc ggttatctgg
     8941 cggtacgttt gacgggcggc agtgcggaaa acgcggcgaa acgcgggcac ctgaccgcaa
     9001 gtaccgttat tcagtatcgc ggcgcgatta tcccgcgtga ggcgatgcca gcgtaaacgc
     9061 agcatatgca aaaggctgga taaagtgtta cccctctatc cagctcatcc ataaatacct
     9121 gatggttgtg ctaactcatc accatcaggc attactgtgc aggtggaata ttcgtcactt
     9181 caggatgtac gtcatccgtg gcgacagagg tggtcgcagc ggcggtagat ggggccatga
     9241 tttgatccca ggccgcctgc aacgctttca tgttaaattc cggctcgcct ttcggctgca
     9301 gcagtattaa cgccatatca ttcgacaact gctgacgcag atcctgattt aacatctcca
     9361 cagtcaggct gttaaggaaa tcctgccgca gtttctgata ctgctccggc gcgatatcga
     9421 cgacctgatt ttgcaacgaa cgcatccgct ggcccatcag aatatcggta tcagcccgtg
     9481 cataggcggc aaacagtttc tgcagctcca gtttcttttg cgccactaac gcattgaact
     9541 cttcttccgg cagaccttta tcgcgaacct tcgccagttc acgcgccacc agattaaggt
     9601 tgctgttcag cttgtcgttt ggtgattcga tgttgatggc acactgcgca cgcagataca
     9661 gcacacggca gtcaaatcca agaccgatgt ctttgctgtc actggcgctt aacgcttgct
     9721 gaacatgcca gaacagcgcc tcgcgggcca ggtccgcacg ccagtagcgc agcagtgcgg
     9781 ctgattcacg aatcggctgc cacggcgtat cccacatgat agataaccgg tcctgacgca
     9841 ccgcgtcagt cataatactc accgcttccg cacgcagcgg agaaagcgtc ggcaccggag
     9901 ctggcgtttc acgtttgcct ttcagttcgc caaacgtttt gttgatttgg tcgacaaccg
     9961 agcgcgcatc cacgtttccc accaccagta gcgtcattgc atccggggtg taccatttct
    10021 gatagaaatc tttaattttt tccgcttcaa cgggttgttt cagcggatcg gcaggatcgt
    10081 gacctaacaa ggttgaccct ttcagacgat agcgccacca gccctcttta gtatcggcag
    10141 gccaggttgc caccatgtcc tgactttgca gcgcgtggtt gatggtttct ggtgtgatgg
    10201 tcaatttgcc agtggcattt gccagataag agagcgcttc tttcagcaaa tcgttacggt
    10261 tattgggcaa actcagatta aacagcgtgg tgtcataaga gacaattacc ggcggcatcg
    10321 ggcgtttagg gtcgatcccc tgctgccaca atgaacgcgc ctgtgctgcg tcaaggccac
    10381 cgctttgcgt tagcgcaata cgagggatgg cgtgactgta accgctctgt tgtgtacttt
    10441 cggcgagcga accggtatta accagcaggc gaatttcaac acgatcgctg ggacgctggg
    10501 gggtggtcag cacttgccac tgtaaaccgt tggaaagcgt cccctgttgc catgctggat
    10561 caggctggag cgcatctgcc tgcacatagc cagcagtggc catcatcagc aaaccgcccg
    10621 ctaaaagtcg aatttttatg ccctgcatgt gaacccctga tcaactatcc tggtaataaa
    10681 aaaactgccc gttgtcggtc agtctaaatg acgttaaaaa cacttcgtgt tagaccgcaa
    10741 gaacatgaaa atgtcacgga agaagtgaaa taaacctgaa ctcacccagg tggtaagttc
    10801 aggcacaggg gtcaattatg cgcaaacacc cgcactcggg gaagggagtg cgggcataag
    10861 tggtgagatt aagaggataa ttcgtgcgtt ttgccatccg gcgcacgatt attcagcaca
    10921 tcgtccagtt ttttgtggtc cagttctttc acccacttag caacgacaat ggtcgctacg
    10981 ccgttaccga ccaggttagt tagcgcacga gcttctgaca taaagcggtc gataccgagg
    11041 atcagcgcca gacccgctac cggcaaatgg cccaccgcag agagcgtcgc cgccagcacg
    11101 ataaagccac tacccgttac ccctgccgcc cctttagaag aaagcagcaa cacgattaac
    11161 agcgtgattt ggtggacgat atccatctga ctgttagtgg cctgggcgat aaacaccgcc
    11221 gccattgtca ggtatatcga tgtgccatca aggttaaacg agtagcctgt cgggatgacc
    11281 agccccacca ccgatttacg gcagccgagt ttctccatct tgtcgagcat acgcggcagc
    11341 gccgactcgg aagatgaagt ccccagtaca atcagcagtt cttcacggat gtagcggata
    11401 aatttgaaga tactgaaacc agtcgcttta gcgattgaac ccaataccag caccacaaac
    11461 aggatacagg taatgtagaa acagataatc agctgcccca gttgcaccag tgtgccgacg
    11521 ccgtatttac cgatggtaaa cgccattgcc ccgagcgcac caataggtgc cagacgcatg
    11581 atcatattga tgatgccgaa gatgacctgc gagaaacttt cgatcacgtt aaaaatcagt
    11641 tggcctttgc tgcccagacg gtggagcgca aaaccaaaca gtacggcaaa cagcagcacc
    11701 tgcagaatgt taccgctggc aaatgcgcca atgacgctcg ccgggatgac atccataatg
    11761 aaggcgacaa tgccctggtc tttcgcctga tcggcgtaaa ccgctaccgc tttcgcatca
    11821 agcgttgccg gatcgacgtt cattccggca ccaggctgca cgacgttaac gatgataaga
    11881 ccaataatca gcgcgatggt actgacaatt tcaaagtaaa gcagtgcgac tgcgccggta
    11941 cgaccgaccg ccttcatgct ttccatgccc gcaatgcccg ttacgacggt acaaaagatg
    12001 acaggagcga tgatcatctt aatgagctta acgaagccgt cgccaagcgg tttcatttgc
    12061 tcgcctattt caggatagaa atggccaagg agaataccaa tggctatcgc tgtcaggacc
    12121 tgaaagtaaa ggcttttaaa cagagaggtt ttcatagggt gtcctttagt aaaaccacag
    12181 gtct
//
LOCUS       DAJTSI010000117        12155 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000117 DAJTSI010000000
VERSION     DAJTSI010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12155
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.117"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(128..556)
                     /gene="mxiM"
                     /locus_tag="OO327_002972"
     CDS             complement(128..556)
                     /gene="mxiM"
                     /locus_tag="OO327_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCR6019429.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            complement(540..947)
                     /gene="mxiM"
                     /locus_tag="OO327_002973"
     CDS             complement(540..947)
                     /gene="mxiM"
                     /locus_tag="OO327_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCR6019430.1"
                     /translation="MINQINASNALQQRLNSEEVVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            complement(940..1635)
                     /gene="mxiN"
                     /locus_tag="OO327_002974"
     CDS             complement(940..1635)
                     /gene="mxiN"
                     /locus_tag="OO327_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCR6019431.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            complement(1577..2104)
                     /locus_tag="OO327_002975"
     CDS             complement(1577..2104)
                     /locus_tag="OO327_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCR6019432.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            complement(2101..2826)
                     /gene="mxiJ"
                     /locus_tag="OO327_002976"
     CDS             complement(2101..2826)
                     /gene="mxiJ"
                     /locus_tag="OO327_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCR6019433.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            complement(2832..3125)
                     /gene="mxiI"
                     /locus_tag="OO327_002977"
     CDS             complement(2832..3125)
                     /gene="mxiI"
                     /locus_tag="OO327_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCR6019434.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            complement(3138..3389)
                     /gene="mxiH"
                     /locus_tag="OO327_002978"
     CDS             complement(3138..3389)
                     /gene="mxiH"
                     /locus_tag="OO327_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HCR6019435.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            complement(3397..4512)
                     /gene="mxiG"
                     /locus_tag="OO327_002979"
     CDS             complement(3397..4512)
                     /gene="mxiG"
                     /locus_tag="OO327_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCR6019436.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKKIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            complement(4521..4979)
                     /gene="ipgF"
                     /locus_tag="OO327_002980"
     CDS             complement(4521..4979)
                     /gene="ipgF"
                     /locus_tag="OO327_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCR6019437.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            complement(4979..5341)
                     /gene="ipgE"
                     /locus_tag="OO327_002981"
     CDS             complement(4979..5341)
                     /gene="ipgE"
                     /locus_tag="OO327_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCR6019438.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            complement(5351..6967)
                     /locus_tag="OO327_002982"
     CDS             complement(5351..6967)
                     /locus_tag="OO327_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCR6019439.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            7281..8765
                     /gene="icsB"
                     /locus_tag="OO327_002983"
     CDS             7281..8765
                     /gene="icsB"
                     /locus_tag="OO327_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCR6019440.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            8778..9167
                     /gene="ipgA"
                     /locus_tag="OO327_002984"
     CDS             8778..9167
                     /gene="ipgA"
                     /locus_tag="OO327_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCR6019441.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            9183..9809
                     /gene="ipgB1"
                     /locus_tag="OO327_002985"
     CDS             9183..9809
                     /gene="ipgB1"
                     /locus_tag="OO327_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCR6019442.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            9866..10333
                     /gene="ipgC"
                     /locus_tag="OO327_002986"
     CDS             9866..10333
                     /gene="ipgC"
                     /locus_tag="OO327_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCR6019443.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            10339..12081
                     /gene="ipaB"
                     /locus_tag="OO327_002987"
     CDS             10339..12081
                     /gene="ipaB"
                     /locus_tag="OO327_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCR6019444.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
BASE COUNT         4058 a         2430 c         1708 g         3959 t
ORIGIN      
        1 tggagccagc tcaattatgt tacgttcttt atcttttaca agctcaacgt taactggttc
       61 atgtccacta gagtaaatgt aaaacatatt acttgtattt agacctttat atttactcac
      121 aatacaatta tatccctcga attaatttta atttaacttc cccatccttc tctataagga
      181 tggtatcatt tatcttatta ccttttaaaa caaccccgtc tttcagcata gaaaatagtt
      241 tatctatagc tatcttttgt gcatccatga agcacccatc tacttcctta acattaaagt
      301 tgcccatgaa tttacctgca ttaaaactat aaaccgcctt accactaata catttagagt
      361 aaacatttat acttttattg gttgtattaa acgaccataa taaatcattt ggaatagaaa
      421 aataatcctt acttacagga acaatatgcc attctttttc agaattagat gatgacttta
      481 aagcacaccc actcagtgtt aatagcaata tacttaaaat aaatattttc aacttattac
      541 taccatgtcg aatcatctgc ctctctccct gcttcaaaga cactaattaa tgaatcgaag
      601 gttgtagtct taatatagaa atcaatattg ttatatatgg acaatgatgc ataaaatccc
      661 tcatgcaaat cgacatattg atttgcatct atgagtttat taagaagtga acttttttgt
      721 gcagctgtaa gataaggaaa acgtgaattg aataattgtt caatcttatt gtgcagttca
      781 tcattatcta tggaatttaa ttcactctgt gagaaatatt gcattatttc atagattata
      841 tcctcatcaa atgattgcga agatgataat ctctcattta aattaacaac ttcttcacta
      901 tttaatcttt gctgcagagc attgcttgca tttatttgat taatcattaa caggattctc
      961 ttttaacaag tcttctgcaa tttgtctcag tttcttatgt gctaactctt tgaaatattt
     1021 tttatcattt tttgttaatt tttccgcaag ttctatttta acacctgata ttacatcctg
     1081 aggactaaac tccactactt gattaccact acagaatata taattattcc cttcagaata
     1141 ttttaaaata atcttgacat ctgagcgata tttatggaga tcaagttcta actttttcct
     1201 cagtgctaaa ttacacttgg gcaaaaccac ttttaattct gttactgtat tacgcaacga
     1261 actaagccac tgctccaata aagtgctaat gatcctctca tcatgtaaat tttctgaaag
     1321 caaactagtt atatagtcct caatatttct tctattttca ttttgcttta acttccattc
     1381 acaaatatag tcaataatat gctcaaaagc agtctgtata ccaatttgat aaccatcaca
     1441 tacagcatct atacgcagtg attcagcttt tttcgtagcc tcctttataa cacaaattgc
     1501 tttcttctta ctctcagtgt ctctatcttt tatggtttta cataattcct tttcaattct
     1561 ctttataaca acaccatcat aggcatgatg tctggatacc ggtaaagtgc ctttttgcat
     1621 attgcatacc ttcatttaat cgggacaaat ttctttctac aggcgtataa tttccctcca
     1681 aagcaattgg attaaataat aagtttattc tttcatatat accaacagat atattttcct
     1741 tccacgcaat aagatgccaa aaaccacttc gaatagcggc ctccccttgc ttactgattt
     1801 tgcccccata ataactaata accatctcac tatgattaat taaccgagac tcttgactac
     1861 cgataaaata agcaacagct ggaagtaaat tccagttggc aacaaacata gcggttaccc
     1921 cgcttaaagg ttcaatatcg caatttagat catatttatt aattatgaga ttattaatct
     1981 cacttctgat tacgccattt tctaacagtt cagaaggcaa attcaaccga tttctattta
     2041 tatagaacgc tggatcaaaa attattgaaa gatatttttt ataaattcca tccattctta
     2101 tcatattttg tttctcttga accaccctgt tttgaatgcc cataccaaaa gaatgacaac
     2161 tagtacagcc atcccgagaa ataaatatat tacttcattt gttaaaaatt ccgatttaac
     2221 ttccttaaca ggttgtacat ttgtataaac atattcttct ttcggagtta atatgacaga
     2281 tatattttca tacttaacat cagaaaaggt gtttttcaaa aatcgcttaa tattactaac
     2341 taatagttca gactctttcg gtgagtcata tatagcgatt actgatatat gcatcggttt
     2401 tgaagatata tttttttctt caagatcata gctaacatgt atttttgccg aaataacacc
     2461 accaatagaa actaaagact gttccagccg ttgctcaata gcactatata aacgggcctt
     2521 ttcagctctt ggagaagaca ctaatgaatc tgtaggaaac atttgtgaga tatcaactct
     2581 ctccggattt ggcaaatcgt acatgcgcat caaatcaact gccgatgcaa atgtcccctt
     2641 ttcgacttgt accgagatcc cctgtttacc tccatcaact tttctagcag taatattatg
     2701 gcgttctagc acagatatta tttcatttgc ctgtctttga gataaattag aaattaactc
     2761 ttcacgttgc tcacatccaa tcaacatcag caacaagaat aaaataaaac ctttatacct
     2821 aatcatataa attaagactt taataaagtt tcaacagccg aaactgtttt tcttgctaac
     2881 gtgccagcta atgatactcc tattgaataa tttgagagcg tcgtctgtaa cttggccaaa
     2941 gattctgggt ttaatgattc tggattgctt accatctcca ttatttgtga tacttgaata
     3001 tctgtatcca tcttaatatc actatattta gccgacacga cgtcttccag acttgatatc
     3061 tcttgagatt gaaaatcact ggctttgata atatcaacct gattgactgg ataaatgtaa
     3121 ttcatgaatg ctccctatta tctgaagttt tgaataattg cagcatcaac atccttaatc
     3181 actttcactg tattggattg cgcgttccta tataatgtat attcagataa tttactttgg
     3241 tattcagcca gcaactgtgg attcgaagga tttttagcta atttatctag tgccaatgtt
     3301 agttcacctt gtaatgtttg agttccatca tcaaaagttt cagataatga actcaatgtc
     3361 caatcatcat tcggtactgt aacactcatt ttatcctcac ttatttttat ctaaaaaaaa
     3421 ccagtgttta tcattcaaca ttacataact gtctttgctg ttaagatatg atttaccttt
     3481 aaaatcatca tctatcaaaa gtacagaaaa ctcaatatac tgatctccat atattttttt
     3541 atgttcagaa atcagtctat taatgagctc aagcttttca tctgtcagtt cttctctgac
     3601 agaatatgta actttattat tttcacaaat ctctttatac tggacattta cctttgtgaa
     3661 aataccttta gcatcccccc ttgcattatg atcgctaaga tagttgaatt taatattttt
     3721 gatatacgga aattcaacaa tcagagcatt tttcaattta tcaagttcgg tatcttttga
     3781 actatttctt tctttactta aaaacaccag taattccgct ttatcactca ctaaattcaa
     3841 cacatacaaa ttgataaatg ggaatcgttg attgatatat tgttgaattc tatttatttc
     3901 tttgtttgac accagaatat atctgcattt ggtaaatccg gttttattta aggctacaga
     3961 cgcccatacc gcatcatttt gtgtttcggc aagaatataa ttgcactgtc cagtgttgac
     4021 tattccatat ctcttatcat ctataatttc agcaatttct tttacttcat ctttctttaa
     4081 agagagggaa aaaattataa ttaacacaac aataacagca aagaaaaaaa acaccgagtg
     4141 gttcttatac attccgttta gtatatggcc tctcgattta tcctccctca tatttttaag
     4201 gtgaaaagaa atcccgtcat attcaaatga attaaattgt gaattgatat caatttgctc
     4261 acctttttca tcactaaatt gacagatatt atctttgctt atggtcaaat aaattatacc
     4321 atccgattta ctatctgtta caggaataat attttctggg aaattgtcgt ttccaaactc
     4381 aagtgtttct atcgtccgtc cgagaacaat taagttattc ccataatata acggaaactc
     4441 atccccgaca ccgtttgtga gtttcactaa taacctgaat ggtgcaagat ttgagttctt
     4501 tgcctcagac ataaatcctc ttatatcctt cgattattct gcttgctctc tgctgctatc
     4561 cgcaaatagc gtctatagat atcctctgcg tactttaatc tttctttttt ctttttcgga
     4621 gacgttcctg cattataggc tccaacagcc tcccaccctc ttccatacat catcataaac
     4681 tcatttaata actttgctgc ataatgtaca gaaagacatg gatgactaat tagcatttcc
     4741 tcagaatatc ccatttctct aagtctttta gaatgaaagt catttatttg cattatacca
     4801 taatctttac ttccattgtt attaacattg actgcagatt tattaaatcc ggattctttt
     4861 tccgcaatcg cttttaacaa acttgatgga atattgtacc tttcaccagc cttatcccaa
     4921 caatcagctc tccctaaata tgggataaaa cacagcaaaa taaatacgaa acgggacatt
     4981 aatacccctt cattcttcgc gcaaattcat ccttcagcca acgcacacgc gaaatataat
     5041 attctgtatt aacatataca tcctcgaatt catttatccc ggtaagatct aatcgcgcaa
     5101 ttagatttcc gccttcatca tcagtagcta agcatatctt ttctgagtaa tttaggctca
     5161 acgcatatat aagatcattt atattttcag gcaatgcaca aaaagggcat aataaattta
     5221 ttgaatctcc ttctttttca atatatatta gcacatcatc atcaagcatg attgcttgat
     5281 catcgatatc aattaaaggg atacccaagg ctcggcaaat aacatctgct aaatcttcca
     5341 tatattcctc ttatacaaat gacgaatacc ctttcaccat attccatatt tttgggtccc
     5401 ctattctttc agaataagat agctctaacg aagatagcgg caattttttc atgactttat
     5461 ttccgggcac accagtattc atctcttgga tttccatatt accactattc attagaatag
     5521 tagaaaataa tcttttctct tctgaggata atttactatt taattgagaa aactgacctg
     5581 tttcatgttt tcttattatt tctcttttaa tttcagcatc ctgcatacct gttctgtcct
     5641 tcccactctt acaattccag caaggtacag cacctatagt ataagccaat agagtcattc
     5701 tttgtgacaa tttatacggc tctccattat catttttttg taactttttg gttacaatct
     5761 cttttatctg gttagccaga tatatcacat cattcttaca tggtggattt ttttctattg
     5821 cttctgcagc ccaacctcca atcacaccat ttttgaggaa attatccccc aacaaagaac
     5881 atattgattc atcattaagc ttgtcaacat tcctccagcc taagcccatt tttagcgcta
     5941 attcatttac accaaagtta aatgtcacca cttttagatt aacgcttact tctttcagaa
     6001 gaccatcact attccgaatt aataatttcg ttggcttccc ccttttagag tttaaccctt
     6061 ttaatgcgtt cacctgatct tttaacatac tctcctctcc cccggttaaa ctggtcggcg
     6121 tcagaagaga agtcgaaaca atctttaaat ctactgtttt accagacaaa gcctgtgata
     6181 ataactctgg tctgctatat aatgctgcgc ttactaactc ctcagcttta ttacgagcag
     6241 caacagctct ttcagatgag ttttttttca atccataggc tgatataact ccatgtctga
     6301 tacccgaaaa aatttccttc ccctcatcat ccaccacttt tgaaagccac atattcgcga
     6361 tatgatcact ctctctcgta gaagcacaac aaattccttt tccattatac tctttgacaa
     6421 atatattttt attccctatc ttcatatgag aggcaggagt taattgaaaa ccataatttt
     6481 tcccatgatg actaatattc ttgtcgaccg gtccccaatt cttattattt aaataatcag
     6541 taaatatttg ttttattttc gtttgcaatt cctttttatt tttaatgcct agctgtgctc
     6601 ttatctcttc acttattact ttattaagct cattaatatg tcttttatta acttcaccaa
     6661 gtttataggt atctaatcgt tccatcatgg ctttaatttt tgacgtatca ccacctgcca
     6721 actttattgc ctcgcaaaaa acttcagctc tgtcacgcgc aaacaaaagg ctttttcctg
     6781 ttaagcttgt ttggttctgt aaatataatc gattaagcgc ttcgatcccc ttatttcttt
     6841 ctccattttt aactctttga tatgaaatca cgcttacacc tttgtgcttt ccggtttctt
     6901 ctgcgccttt ataggaatct ccgctttgaa atgaaacctg atgcaatccc aaattagtta
     6961 tgtgcattta attatcctca gtcataaatg gggcattgat tgcagttttg tacctcgttt
     7021 tacaacaaaa aagatactta ttcaacaggg ttctctcttt ttctataaca ctccgagcct
     7081 gatacatcag gcactttatc ttgtgggatt tcatgatgaa acgagcacta catatgctca
     7141 cgaggtacat attatgtaca gcaaaagaaa atttgattat gttaacaggc tcaagtttct
     7201 aaatttatta attgaatata ttatatacca aatacatttt acatccccac aatcaccaag
     7261 taatggagag ttaataaagt atgatcctca aaattagcaa tttcattgac gcaagcaata
     7321 caaaagggcc tatacgcgtt gaagatacag agcatggacc tatattgata gcacagaaat
     7381 tcaacctaaa ggatctcttt ttcagaacat taagcactat taatgcaaag ataaattcac
     7441 agatattaaa tgaacaacta aaaaattatc gactagagaa tcaaaaatcc ttattgcttt
     7501 tcctcaatac attagctagc gaaaaatctg cagaaagtgc gtttgccgcc tatgaagcag
     7561 ctaaaaactc aattcaacac tctttcacag gtagagacat caaattaatg ttgaataccg
     7621 cagagcgttt tcatggcatc ggtacagcca aaaatcttga aagacattta gtttttcgtt
     7681 gctggggaaa tagaggcata acccatttag ggcatactag tatcagtata aaaaacaatt
     7741 tactacagga accaactcat acctatctca gctggtatcc cggcgggaat gttacaaaag
     7801 atacagaaat aaactacctt tttgaaaaac gctcaggtta cagtgtcgat acctataagc
     7861 aagacaaact aaatatgatt tcagaccaaa cagccgaaag acttgatgca ggccaagagg
     7921 ttagaaactt attaaattca aaacaagatc aaaataacaa caaaaaaata ttttttccca
     7981 gagctaatca aaaaaaagac ccctatggtt attggggggt cagtgctgat aaagtataca
     8041 ttccattatc aggtgataat aaaacaaagg atggtaaaat cagccataac ctttttggcc
     8101 ttgatgaaac aaacatgtca aaatttatat gcaaaaagaa agcagatgct ttcagacaat
     8161 tggcaaatta taaattaatc agtaaatctg aaaactgtgc tggtatggca cttaatgttc
     8221 ttaaagcagg aaattctgaa atatactttc cactccctga cgttaaattg gttgctacac
     8281 ctaacgatgt atacgcgtat gcaaataaag ttaggcaacg cattgaaagt ctcaatcagt
     8341 cttataacga aataatgaag tatattgaat ctgattttga tctttccaga ttgactcaat
     8401 tacgacgcag ctatcttaaa agtttcaata aaattaacct tatccacaca ccaaaaacat
     8461 tcaagccttt atcaatttca ctttacaaac atcctactga aaatgtatct tcagaagact
     8521 ttgatgcggt catcaacgct tgccactcat acttagtaaa atcagcaccc agcaatatga
     8581 ctcgtgtatt aaacgaatta aaaaccgaag caacagataa aaaagaagaa atcatcgaaa
     8641 aatcaataaa aattattgat tattacaatt ctcttaaatc gccagatctt ggaaccaaac
     8701 tttacattca cgacttatta cagatcaata agcttttatt gaataactct cattctaata
     8761 tatagaaggc catagaaatg tgtcgcaaac tatatgataa actttatgaa ataacaggtg
     8821 caaagcttga ttttaacgat aaaaatcaag cctttatatt gctcgaagaa caaatacctg
     8881 tttgtataac agataatgat gaatacatct ttttgaccgg acttcttaat gaacatgaac
     8941 tttttacaga aaatattatt aatccagaac acatactcat ccttaactac tctttgagta
     9001 gagattatgg ttcttctatc tgtcttctcc ctgatactca tcaatgtgtt ttgaccaaga
     9061 aacactacaa aaaatatctt tcacctgatg aactcattga atctttgtat gaatttttat
     9121 tttgtattaa actaaccatt gcaaacatca cttcagaagt gaactaacat atagggggta
     9181 tcatgcaaat tctaaacaaa atacttccac aggttgaatt cgcgattcct cgcccatcat
     9241 ttgattcgtt aagtcgcaat aagcttgtaa agaaaatatt atctgttttt aacttaaaac
     9301 aacgctttcc tcagaagaat tttggatgcc cggtgaatat aaataaaatc agggacagcg
     9361 tcatagacaa aataaaagat agtaattcgg gaaatcaact attttgttgg atgagccaag
     9421 agcgaaccac ttatgtctct tcaatgataa acagaagtat agatgaaatg gccatacaca
     9481 atggagttgt tttgacctca gataacaaaa aaaacatatt tgcagctatt gaaaaaaaat
     9541 tcccggatat aaaacttgat gaaaaatcag cacaaacatc tatcagtcat acagcactaa
     9601 acgagattgc ctcatctggc ctcagagcaa agattttaaa acgctatagc agtgacatgg
     9661 atttgtttaa cacacaaatg aaggatctta caaatctagt atccagttcc gtctacgata
     9721 aaatatttaa tgaatcaaca aaagttttac aaatagaaat aagcgcagag gttctaaagg
     9781 ctgtataccg tcaaagcaat acaaattaaa tcttttatat taaatcttat acttattaaa
     9841 gtaaagcaga caaaaaggag accttatgtc tttaaatatc accgaaaatg aaagcatctc
     9901 tactgcagta attgatgcaa ttaactctgg cgctacactg aaagatatta atgcaattcc
     9961 tgatgatatg atggatgaca tttattcata tgcttatgac ttttacaaca aaggaagaat
    10021 agaggaagct gaagttttct tcaggttttt atgtatatac gacttttaca atgtagacta
    10081 cattatggga ctcgcagcta tttatcagat aaaagaacag ttccaacaag cagcagacct
    10141 ttatgctgtc gcttttgcat taggaaaaaa tgactataca ccagtattcc atactggaca
    10201 atgccagctt cggttgaaag cccccttaaa agctaaagag tgcttcgaac tcgtaattca
    10261 acacagcaat gatgaaaaat taaaaataaa agcacaatca tacttggacg caattcagga
    10321 tatcaaggag taattattat gcataatgta agcaccacaa ccactggttt tcctcttgcc
    10381 aaaatattgg cttccactga gcttggagac aatactatcc aagctgcaaa tgatgcagct
    10441 aacaaattat tttctcttac aattgctgat cttactgcta accaaaatat taatacaact
    10501 aatgcacact caacttcaaa tatattaatc cctgaactta aagcaccaaa gtcattaaat
    10561 gcaagttccc aactaacgct tttaattgga aaccttattc aaatactcgg tgaaaaatct
    10621 ttaactgcat taacaaataa aattactgct tggaagtccc agcaacaggc aagacagcaa
    10681 aaaaacctag aattctccga taaaattaac actcttctat ctgaaactga aggactaacc
    10741 agagactatg aaaaacaaat taataaacta aaaaacgcag attctaaaat aaaagaccta
    10801 gaaaataaaa ttaaccaaat tcaaacaaga ttatccgaac tcgacccaga gtcaccagaa
    10861 aagaaaaaat taagccggga agaaatacaa ctcactatca aaaaagacgc agcagttaaa
    10921 gacaggacat tgattgagca gaaaaccctg tcaattcata gcaaacttac agataaatca
    10981 atgcaactcg aaaaagaaat agactctttt tctgcatttt caaacacagc atctgctgaa
    11041 cagctatcaa cccagcagaa atcattaacc ggacttgcca gtgttactca attgatggca
    11101 acctttattc aactagttgg aaaaaataat gaagaatctt taaaaaatga tctggctcta
    11161 ttccagtctc tccaagaatc aagaaaaact gaaatggaga gaaaatctga tgagtatgct
    11221 gctgaagtac gtaaagcaga agaactcaac agagtaatgg gttgtgttgg gaaaatactt
    11281 ggggcacttt taactatcgt tagtgttgtt gcagcagctt tttctggagg agcctctcta
    11341 gcactggcag ctgttggttt agctcttatg gttacggatg ctatagtaca agcagcgacc
    11401 ggcaattcct tcatggaaca agccctgaat ccgatcatga aagcagtcat tgaaccctta
    11461 atcaaactcc tttcagatgc atttacaaaa atgctcgaag gcttgggcgt cgactcgaaa
    11521 aaagccaaaa tgattggctc tattctgggg gcaatcgcag gcgctcttgt cctagttgca
    11581 gcagtcgttc tcgtagccac tgttggtaaa caggcagcag caaaacttgc agaaaatatt
    11641 ggcaaaataa taggtaaaac cctcacagac cttataccaa agtttctcaa gaatttttct
    11701 tctcaactgg acgatttaat cactaatgct gttgccagat taaataaatt tcttggtgca
    11761 gcgggtgatg aagtaatatc caaacaaatt atttccaccc atttaaacca agcagtttta
    11821 ttaggagaaa gtgttaactc tgccacacaa gcgggaggaa gtgtcgcttc tgctgttttc
    11881 cagaacagcg cgtcgacaaa tctagcagac ctgacattat cgaaatatca agttgaacaa
    11941 ctgtcaaaat atatcagtga agcaatagaa aaattcggcc aattgcagga agtaattgca
    12001 gatctattag cctcaatgtc caactctcag gctaatagaa ctgatgttgc aaaagcaatt
    12061 ttgcaacaaa ctactgcttg atacaaataa ggagaatgtt atggaaattc aaaacacaaa
    12121 accaacccag attttatata cagatatatc cacaa
//
LOCUS       DAJTSI010000118        12151 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000118 DAJTSI010000000
VERSION     DAJTSI010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.118"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            126..995
                     /locus_tag="OO327_002988"
     CDS             126..995
                     /locus_tag="OO327_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCR6019445.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1198..1551)
                     /locus_tag="OO327_002989"
     CDS             complement(1198..1551)
                     /locus_tag="OO327_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR6019446.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1689..3335)
                     /gene="groL"
                     /locus_tag="OO327_002990"
     CDS             complement(1689..3335)
                     /gene="groL"
                     /locus_tag="OO327_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR6019447.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3379..3672)
                     /locus_tag="OO327_002991"
     CDS             complement(3379..3672)
                     /locus_tag="OO327_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR6019448.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3948..5204
                     /gene="yjeH"
                     /locus_tag="OO327_002992"
     CDS             3948..5204
                     /gene="yjeH"
                     /locus_tag="OO327_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR6019449.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5220..5696)
                     /gene="fxsA"
                     /locus_tag="OO327_002993"
     CDS             complement(5220..5696)
                     /gene="fxsA"
                     /locus_tag="OO327_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR6019450.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6033..7469
                     /gene="aspA"
                     /locus_tag="OO327_002994"
     CDS             6033..7469
                     /gene="aspA"
                     /locus_tag="OO327_002994"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR6019451.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7587..8888
                     /gene="dcuA"
                     /locus_tag="OO327_002995"
     CDS             7587..8888
                     /gene="dcuA"
                     /locus_tag="OO327_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR6019452.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9004..9342
                     /gene="cutA"
                     /locus_tag="OO327_002996"
     CDS             9004..9342
                     /gene="cutA"
                     /locus_tag="OO327_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR6019453.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9318..11015
                     /gene="dsbD"
                     /locus_tag="OO327_002997"
     CDS             9318..11015
                     /gene="dsbD"
                     /locus_tag="OO327_002997"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR6019454.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPSGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWPGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11052..11627
                     /locus_tag="OO327_002998"
     CDS             11052..11627
                     /locus_tag="OO327_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6019455.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11734..11809
                     /locus_tag="OO327_002999"
     tRNA            11734..11809
                     /locus_tag="OO327_002999"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11767..11769,aa:Phe,seq:gaa)
BASE COUNT         2930 a         3140 c         2980 g         3101 t
ORIGIN      
        1 ttctgtttat atagagtgag cacggctatt tatgttattt taacaactat tactaccaag
       61 cccatttatc tggcttataa attaaaatta attatttaat tgagaaatgg ttagggagaa
      121 cctacatggc cataagcatc aagggtgtta ataccggtgt tattcgcaag agtaataatt
      181 tcattgcact ggcgctgaaa attaaagaac ctcgcaataa agaatcgttg ttctttatgt
      241 ctgtcatgga actgcgcgac ctgctgattg cgctggaaag tcgtctgcat caaaagcata
      301 aactggatgc tgctgcacgc cttcagtatg agcaagctcg cgataaagtc attaagaaaa
      361 tggcagaaaa catcccagaa attctggttg atgagcttaa aaatgccgat attaatcgtc
      421 gggttaacac gctggaactt accgataatc aaggcgaaaa tcttaccttc gtattaacgc
      481 tacatgacgg tagcaaatgt gagttagtcg tcaatgagtt gcagattgaa atgctggcgc
      541 gagcaatcat tcatgcaatt aataatgcgg aaatgcgtga actggcatta cgtattacct
      601 cactgttaga tttcctgccg ttatacgacg ttgactgcca ggacaatggc aatctggaat
      661 atgacaccta ttcccaaccg gaatggaaac ataacttgtt cgaccattat ctggcggtgc
      721 tttaccgctt taaagatgaa agtggcaaag aacaatttag cggcgcagtg gtgaaaacgc
      781 gtgaagcaac gccgggtaaa gaaatagagg cgatcactcg ccgaatgctt gatttcagcc
      841 cacgactgaa aaaactcgcg ggtgtgccat gtcaggtcta tgttcgcacc gtagcggcga
      901 ataacgccca accgctaacc caggatcaat gcttacgcgc gctgcatcac ttacgtgtac
      961 aatccaccag caaaactgca ccacaggcga aataatctca tctttgctaa tgcgaggctg
     1021 gagccatcca gcctgaaatg actgataacg tgatcgttgg tcatgccgga tgcggcgtaa
     1081 acgccttatc cggcctacaa attctcaagc attcaatcag ttgcaatgcc tgctgtaggc
     1141 ctgataagcg aagcgcatca ggcaataata cgtttgtcac ccagcctctg gatcacatca
     1201 gttcgggcac ttataaacct gaccgataat ctggctatcc gtcgggacaa aactggacaa
     1261 cattccctgc gacgggctgc tgatgccata aatcacgtta ccgcccattg cagccgcctg
     1321 gttgcgcaga tcgtttgctg cgccgcgcat agaaccgccc tcttctccgt gttgcccgga
     1381 aagccagttg ctttgcttac ctgtcgcagt accaatcagc tggcactctg cgcctggctg
     1441 ctcgtccaca atgcgtacac tctgaccggc agcactcaat tcgttgctgg agctacaacc
     1501 cgccatcagt agcgcggcac cgacaatccc tgctaagtat tttacgtgca tgttattccc
     1561 cataatcaat gagctggacg ccactcgcgt catccgtgtc tgaatcttat actagaaaga
     1621 tgaccaaaag aaaaaccccc agacatttct gcccgggggt ttgtttattt ctgcgaagtg
     1681 cagggcaatt acatcatgcc gcccatgcca cccatgccgc ccataccgcc agcagcgcct
     1741 aagtcagctg catcgttttt cggcaggtcg gtaaccatgc attcggtggt gatcatcagg
     1801 ccagccacag aagctgcgta ctgcagagca gaacgggtta ctttggttgg gtccaggata
     1861 cccatgtcga tcatgttgcc gtattcttcg gttgctgcgt tgtaaccgta gttgccgtcg
     1921 ccgcctttaa cggtgttagc aacaacagac ggttcttcgc cgcagttcag gacgatctga
     1981 cgcagcggag cttccattgc acgcagtgca actttgatac ccacgttctg gtcttcgttc
     2041 tgaccacgca ggtcagccag tttagacgct acgcggatca gcgcaacacc accaccagca
     2101 accacgcctt cttctaccgc agcacgggtc gcgtgcaggg catcttcaac gcgtgctttt
     2161 ttctctttca tttcaacttc ggtagcagca cctactttga taactgcaac gccgcctgcc
     2221 agtttcgcta cgcgctcctg cagtttttca cggtcgtagt cagaagttgc ttcttcaatc
     2281 tgctgacgga tctgagcaac acggccctgg attgcagctt cttcacccac gccatcgatg
     2341 atagtggtgg tgtctttgtt gatcacaaca cgtttagcct gacccaggtc ttccagggtt
     2401 gctttttcca gctccatacc gatctcttca gagattacgg taccgccagt cagggttgcg
     2461 atatcctgca gcatagcttt acgacgatcg ccgaagcccg gagctttaac cgcagcaact
     2521 ttcacgatgc cacgcatggt gttaacaacc agagttgcta gcgcttcgcc ttcaacatct
     2581 tcagcgatga tcagcagcgg tttgcctgct ttggcaacag cttccagaac cggcagcatt
     2641 tcgcggatgt tggagatttt cttgtcagcc agcaggatga acgggctttc cagttctact
     2701 gcgccagttt ccggcttgtt gatgaagtaa ggagacaggt agccacggtc gaactgcata
     2761 ccttcaacca cgtccagttc gtcctgcaga ccggtaccgt cttcaacggt gataacgcct
     2821 tctttaccga ctttgtccat cgcttcagcg atcagtttac ctacggtttc gtcggagtta
     2881 gcggagatgg taccaacctg agcaatcgct ttagaatcag agcacggtac ggacagcgct
     2941 ttcagttctt caactgcagc ggtaaccgct ttgtcgatac cacgtttcag gtccatcggg
     3001 ttcatgcccg cagcaacagc tttcagacct tcagtgatga tagcctgagc cagtacggtt
     3061 gcagtggtgg taccgtcgcc tgcagcgtcg ttcgctttgg aggcaacttc tttcaccatc
     3121 tgcgcaccca tattttcgaa cttgtcttcc agttcgattt cacgagcaac ggaaacacca
     3181 tctttggtga tggtcggtgc accgaaagat ttatccagaa ctacgttacg gccttttgga
     3241 ccgagggtaa ctttcactgc atctgccagt acgtttacgc cgcgcagcat tttcacacga
     3301 gcgtcgttac cgaattttac gtctttagct gccattatct ttattcctta aattcgtatg
     3361 ttcagtgtcg tgcgcggatt acgcttcaac aattgccaga atgtcgcttt cggacatgat
     3421 caacacttct tcattgtcga tcttctcgga tttcacaccg tagccatcgt tgaaaataac
     3481 gatgtcgcca actttcacgt ccagcggctt cacttcgcca ttttcaagga tacggccatt
     3541 gccgacagcc agcacttcgc cgcgggtgga tttagccgct gcagagccgg tcagaacgat
     3601 gccgccagca gatttagttt caacttcttt acgcttgacg atcacgcgat catgcaatgg
     3661 acgaatattc attgataact ctcctttgag aaagtccgta tctgttatgg gtgacgccgg
     3721 agcttacgtg gtttcccggc tggtgaccag agaaatgggg atgaggcttc gccccttcaa
     3781 gggggaaaaa gaaaaaaaat tctgatcagc acaaaatcgg gtgaaaaccc tgattcacct
     3841 cacatttcat cgcaatttct tcatcgccgt cataagcgaa tctgattgtg ctaccatcga
     3901 aaatctacgc agttgcccaa aatttgggcg caatcggata tcaacccatg agtggactca
     3961 aacaagaact ggggctggcc cagggcattg gcctgctatc gacgtcatta ttaggcactg
     4021 gcgtgtttgc cgttcctgcg ttagctgcac tggtagcagg caataacagc ctgtgggcgt
     4081 ggcccgtttt gattatctta gtgttcccga ttgcgattgt gtttgcgatt ctgggtcgcc
     4141 actatcccag cgcaggcggc gtcgcgcact tcgtcggtat ggcgtttggt tcgcggcttg
     4201 agcgagtcac cggctggctg tttttatcgg tcattcccgt gggtttgcct gccgcactac
     4261 aaattgccgc cggattcggc caagcaatgt ttggctggca tagctggcaa ctgttgttgg
     4321 cagaactcgg tacgctggcg ttggtgtggt atatcggtac tctcggtgcc agttccagtg
     4381 ctaatctaca aaccgttatt gccggactta tcgtcgcact gattgtcgct atctggtggg
     4441 cgggcgatat caaacctgcg agtatcccct tccccgcgcc aggaaatatc gaacttaccg
     4501 ggttatttgc tgcgttatca gtgatgttct ggtgttttgt cggtctggaa gcatttgccc
     4561 atcttgcctc ggaatttaaa aatccagagc gtgattttcc tcgtgctttg atgattggtc
     4621 tgctgctggc aggattagtc tactggggct gtacggtagt cgtcttacac ttcgacgcct
     4681 atggtgaaca aatggcggcg gcagcatcgc ttccaaaaat tgtagtacag ttgttcggtg
     4741 taggagcgtt atggattgcc tgcgtaattg gctatctggc ctgctttgcc agtcttaaca
     4801 tttatataca gagcttcgcc cgcctggtct ggtcgcaggc gcaacataat cctgaccact
     4861 acctggcacg cctctcttct cgccatatcc cgaataatgc cctcaatgcg gtgcttggct
     4921 gctgcgtggt gagcacgttg gtaattcatg ccttagagat caatctggac gctcttatta
     4981 tttatgccaa tggcatcttt attatgattt atctgttatg catgctggca ggctgtaaat
     5041 tattgcaagg tcgttatcga ctactggcgg tggttggcgg gctgttatgc gttctgttac
     5101 tggcaatggt cggctggaaa agtctctatg cgctgatcat gctggtgggg ttatggctgt
     5161 ttctgccaaa acgaaaaacg ccggaaaatg gcataaccac ataatccggc gtttcgacat
     5221 taatcctggc gatcgtcttt atgatcaagg cggtcgcggt catcatcctt tcgctgatac
     5281 tcaccatcaa aagtattacc gccaccggtc ccggcgctaa aaccgccgcc tggcatgcga
     5341 gaaaagcgca aatgtggcat caacttcact gtcagatgct tttgcaccgg cggcaataaa
     5401 agcagaagac cgaggaagtc ggtgaaaaag cccggtaata aaagcagcaa accggcaata
     5461 atcagcgaaa cacttttaat catctccgcc gcagggtttt cacccgccgc cattttttgc
     5521 tgcatcagca cgaaattctt aaagccctgg ttacgtacca gtgacatacc gataactgac
     5581 gtgaatataa ccagcacgag ggtcagcaat acccccaata catgggcaac ctgaataaag
     5641 attgaaatct caatatagac ataaaggaaa atggcaataa aaggtaacca gcgcaaaggt
     5701 ttctcctgta atagcagccg gttaaacccg gctacctgaa tgggttgcga atcgccttca
     5761 gcttatattg tggtcattag caaaatttca agatgtttgc gcaactattt ttggtagtaa
     5821 tcccaaagcg gtgatctatt tcacaaatta ataattaagg ggtaaaaacc gacacttaaa
     5881 gtgatccaga ttacggtaga aatcctcaag cagcatatga tctcgggtat tcggtcgatg
     5941 caggggataa tcgtcggtcg aaaaacattc gaaaccacat atattctgtg tgtttaaagc
     6001 aaatcattgg cagcttgaaa aagaaggttc acatgtcaaa caacattcgt atcgaagaag
     6061 atctgttggg taccagggaa gttccagctg atgcctacta tggtgttcac actctgagag
     6121 cgattgaaaa cttctatatc agcaacaaca aaatcagtga tattcctgaa tttgttcgcg
     6181 gtatggtaat ggttaaaaaa gccgcagcta tggcaaacaa agagctgcaa accattccta
     6241 aaagtgtagc gaatgccatc attgccgcat gtgatgaagt cctgaacaac ggaaaatgca
     6301 tggatcagtt cccggtagac gtctaccagg gcggcgcagg tacttccgta aacatgaaca
     6361 ccaacgaagt gctggccaat atcggtctgg aactgatggg tcaccagaaa ggtgaatatc
     6421 agtacctgaa cccgaacgac catgttaaca aatgtcagtc cactaacgac gcctacccga
     6481 ccggtttccg tatcgcagtt tactcttccc tgattaagct ggtagatgcg attaaccaac
     6541 tgcgtgaagg ctttgaacgt aaagctgtcg aattccagga catcctgaaa atgggtcgta
     6601 cccagctgca ggacgcagta ccgatgaccc tcggtcagga attccgcgct ttcagcatcc
     6661 tgctgaaaga agaagtgaaa aacatccaac gtaccgctga actgctgctg gaagttaacc
     6721 ttggcgcaac agcaatcggt actggtctga acacgccgaa agagtactct ccgctggcag
     6781 tgaaaaaact ggctgaagtc actggcttcc catgcgtacc ggctgaagac ctgatcgaag
     6841 cgacctctga ctgcggcgct tatgttatgg ttcacggcgc gctgaaacgc ctggctgtga
     6901 agatgtccaa aatctgtaac gacctgcgct tgctctcttc tggtccacgt gccggcctga
     6961 acgagatcaa cctgccggaa ctgcaggcgg gctcttccat catgccagct aaagtaaacc
     7021 cggttgttcc ggaagtggtt aaccaggtat gcttcaaagt catcggtaac gacaccactg
     7081 ttaccatggc agcagaagca ggtcagctgc agttgaacgt tatggaaccg gtcattggcc
     7141 aggccatgtt cgaatccgtt cacattctga ccaacgcttg ctacaacctg ctggaaaaat
     7201 gcattaacgg catcactgct aacaaagaag tgtgcgaagg ttacgtttac aactctatcg
     7261 gtatcgttac ttacctgaac ccgttcatcg gtcaccacaa cggtgacatc gtgggtaaaa
     7321 tctgtgccga aaccggtaag agtgtacgtg aagtcgttct ggaacgcggt ctgttgactg
     7381 aagcggaact tgacgatatt ttctccgtac agaatctgat gcacccggct tacaaagcaa
     7441 aacgctatac tgatgaaagc gaacagtaat cgtacagggt agtacaaata aaaaaggcac
     7501 gtcagatgac gtgccttttt tcttgtgagc agtaacttaa aaataacaat ctaatatcaa
     7561 cttgttaaaa aacaaggaag gctaatatgc tagttgtaga gctcatcata gttttgctgg
     7621 cgatcttctt gggcgccaga ttggggggaa taggtattgg ttttgcaggc ggattggggg
     7681 tgctggttct tgccgctatt ggcgttaaac ccggtaacat cccgttcgat gtcatttcca
     7741 ttatcatggc ggttatcgcc gctatttctg ccatgcaggt tgctggcggt ctggactatc
     7801 tggttcatca gacagaaaag ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac
     7861 cgatcgtgac ctatttcctg actatctttg ctggtactgg caacatctct ctggcgacac
     7921 tgccagttat cgctgaagtt gcgaaggaac aaggcgttaa accttgccgt ccgctctcta
     7981 ctgcagtggt atccgcgcag attgcgatca ccgcatcgcc aatctcagcg gcagtggttt
     8041 acatgtcttc cgtgatggaa ggtcatggca tcagctacct ccatctgctc tccgtggtca
     8101 tcccgtccac cctgctggcg gttctggtga tgtccttcct ggtcactatg ctgttcaact
     8161 ccaaactctc tgacgatccg atttatcgca agcgtctgga agagggcttg gttgaactgc
     8221 gcggtgaaaa gcagattgaa atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc
     8281 tgggcgtagt tggcgtggtt atctatgcaa tcatcaacag cccaagcatg ggtctggttg
     8341 aaaaaccgct gatgaacacc accaacgcaa tcctgatcat catgctcagc gttgcaactc
     8401 tgaccaccgt tatctgtaaa gtcgataccg acaacatcct caactccagc accttcaaag
     8461 caggtatgag cgcctgtatt tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt
     8521 ccaacaacat cgactggatc aaagataccg ctggtgaagt gattcagggt catccgtggc
     8581 tgctggccgt catcttcttc tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa
     8641 aagcactgat gccgatggct ctggcactga acgtttcacc gctgaccgct gttgcttctt
     8701 tcgctgcggt gtctggtctg ttcattctgc cgacctaccc gacgctggtt gctgcggtac
     8761 agatggatga cacgggtact acccgtatcg gtaaattcgt cttcaaccat ccgttcttca
     8821 tcccgggtac tctgggtgtt gccctggccg tttgcttcgg cttcgtgctg ggtagcttca
     8881 tgctgtaatg atcaatcgcg gggcgttcac gccccgcttt ctttcccgcc gactaacatc
     8941 cttcccccgt ccgttgtata gtgacctctc tcttgcggtt ccatctgttc ttgcgaggtg
     9001 tttatgcttg atgaaaaaag ttcgaatacc acgtctgtcg tggtgctatg taccgcaccg
     9061 gatgaagcga cagcccagga tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc
     9121 gcgaccttga tccccggcgc tacctctctc tattactggg aaggtaagct ggagcaagaa
     9181 tacgaagtgc agatgatttt aaaaactacc gtatctcacc agcaggcact gctggaatgc
     9241 ctgaagtctc atcatccata tcaaaccccg gaacttctgg ttttacctgt tacacacgga
     9301 gacacagatt acctctcatg gctcaacgca tctttacgct gattctgcta ctttgcagca
     9361 cttccgtttt tgccggatta ttcgacgcgc cgggacgttc acaatttgtc cccgcggatc
     9421 aagcctttgc ttttgatttt cagcaaaacc aacatgacct taatctgacc tggcagatca
     9481 aagacggtta ctacctctac cgtaaacaga tccgcattac gccggaacac gcgaaaattg
     9541 ccgacgagca gctgccgcaa ggcgtctggc atgaagatga gttttacggc aaaagcgaga
     9601 tttaccgcga tcggctgacg cttcccgtaa ccatcaacca ggcgagtgcg ggagcgacgt
     9661 taactgtcac ctaccagggc tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa
     9721 ccgttccgtt aagcgaagtg gtcgccaaca acgcagcgtc acagcctgtg tctgtttcgc
     9781 agcaagagca gcacaccgcg caattgccct tttccgcgct ctgggcgttg ttgatcggta
     9841 ttggtatcgc ctttacgcca tgcgtgctgc caatgtaccc actgatttct ggcatcgtgc
     9901 tgggtggtaa acagcggctc tccactgcca gagcattgtt gctgaccttt atttatgtgc
     9961 aggggatggc actgacttac acggcgctgg gtctggtagt tgccgccgca gggttacagt
    10021 tccaggcggc gctacagcac ccatacgtgc tcattggcct cgccatcgtc tttaccttgc
    10081 tggcgatgtc aatgtttggc ttgtttactc tacaactccc ctcttcgctg caaacacgtc
    10141 tcacgttgat gagcaatcgc caacagggcg gctcacctag cggtgtgttt gttatggggg
    10201 cgattgccgg actgatctgt tcaccatgca ccaccgcacc gcttagcgcg attctgctgt
    10261 atatcgccca aagcgggaac atgtggccgg gcggcggcac gctttatctc tatgcgttgg
    10321 gcatgggcct gccgctgatg ctaattaccg tctttggtaa ccgcttgctg ccgaaaagcg
    10381 gcccgtggat ggaacaagtc aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct
    10441 tcctgctgga gcgagtgatt ggtgatgtat ggggattacg cttgtggtcg gcgctgggtg
    10501 tcgcattctt tggctgggcc tttatcacca gcctacaggc taaacgcggc tggatgcgcg
    10561 tggtgcaaat aatcctgctg gcagcggcat tggttagcgt gcgcccactt caggattggg
    10621 catttggtgc gacgcatacc gcgcaaactc aaacgcatct caactttaca caaatcaaaa
    10681 cggtagatga gttaaatcag gcgctcgttg aagccaaagg caaaccggtg atgttagatc
    10741 tttatgccga ctggtgcgtc gcctgtaaag agtttgagaa atacaccttc agcgacccgc
    10801 aggtgcaaaa agcgttagca gacacggtct tacttcaggc caacgtcacg gccaacgacg
    10861 cacaagatgt ggcgctgtta aagcatctta atgtccttgg cctaccgaca attctctttt
    10921 ttgacggaca aggccaggag catccacaag cacgcgtcac gggctttatg gatgctgaaa
    10981 ccttcagcgc acatttgcgc gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg
    11041 aggaaaatag cgtgcaacgt gaagatgtac tgggagaagc cctgaaatta ttagaattac
    11101 aagggattgc caacaccacg ctggagatgg ttgctgaacg tgtggattat ccactggacg
    11161 agctacgccg cttctggcca gacaaagagg caatcctcta cgatgcgctg cgttatctta
    11221 gccaacagat agatgtctgg cgtcgtcagc tgatgctgga cgaaacgcaa accgccgaac
    11281 aaaagctgct ggcacgttat caggcgttat cggagtgcgt taaaaacaac cgctatccgg
    11341 gctgtctatt tatcgctgcc tgtacgtttt atcccgatcc tggccaccct attcatcaac
    11401 tggccgatca gcaaaaaagc gctgcctacg atttcaccca cgaactgtta accacgctgg
    11461 aagttgacga tccggcgatg gtagcaaagc agatggaact ggtgctggaa ggctgtttaa
    11521 gccgaatgct ggtgaatcgt agccaggcgg atgtcgacac tgcacatcgg ctggcggaag
    11581 atatcctgcg cttcgcccgc tgccgccagg gtggtgcatt gacctgacag aaacacagaa
    11641 aagaagcgat ttgccgcaat cttaagcagt tgaatcgctt ttactgaaat taggttgacg
    11701 agatgtgcag attacggttt aatgcgcccc gttgcccgga tagctcagtc ggtagagcag
    11761 gggattgaaa atccccgtgt ccttggttcg attccgagtc cgggcaccaa attcatatca
    11821 acggacctcc acggaggtcc gtttttcgtt tcaggacgcc acgatttaag cgttctgcct
    11881 ccaaatcaat tctaccgaac tcaaccagat tctccccaca tcaccagcaa tttgcgagca
    11941 tatcccaatt tgggaaaatt tgttcctgag ctatagcact aactgacgtg aaatgtcgtg
    12001 cggccccgtg atgctgttga acgtcaaatg acgtcatcag gagcgtaacg tatccataaa
    12061 gcacaacacc ggtctgaacg ccgactgatg agattttctg aatgagaaca aagagaaatg
    12121 tgtcagtccg cttgctcatg caaagactaa c
//
LOCUS       DAJTSI010000119        12130 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000119 DAJTSI010000000
VERSION     DAJTSI010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12130
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.119"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OO327_003000"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OO327_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR6019456.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQTLPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="OO327_003001"
     CDS             complement(2138..2584)
                     /locus_tag="OO327_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR6019457.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="OO327_003002"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="OO327_003002"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR6019458.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OO327_003003"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OO327_003003"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR6019459.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="OO327_003004"
     CDS             complement(5347..6009)
                     /locus_tag="OO327_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR6019460.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="OO327_003005"
     CDS             6182..6595
                     /locus_tag="OO327_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR6019461.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OO327_003006"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OO327_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR6019462.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OO327_003007"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OO327_003007"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCR6019463.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="OO327_003008"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="OO327_003008"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR6019464.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OO327_003009"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OO327_003009"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR6019465.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OO327_003010"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OO327_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR6019466.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="OO327_003011"
     tRNA            complement(9861..9948)
                     /locus_tag="OO327_003011"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="OO327_003012"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="OO327_003012"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR6019467.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..>12130
                     /locus_tag="OO327_003013"
     CDS             11489..>12130
                     /locus_tag="OO327_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HCR6019468.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQR"
BASE COUNT         3114 a         3076 c         3163 g         2777 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgtctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggctggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgacgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcgggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaaat ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagtag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccatgaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaga
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt
//
LOCUS       DAJTSI010000120        12114 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000120 DAJTSI010000000
VERSION     DAJTSI010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.120"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..372
                     /locus_tag="OO327_003014"
     CDS             <1..372
                     /locus_tag="OO327_003014"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019469.1"
                     /translation="NTTLMSWDAVLYDLRSEKIISKDNYLTDLNRRHLELVLHTNPNP
                     SGSVTKILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHYQSSSFSRSQFN
                     ALINLLAQQSRFADLKLSNYK"
     gene            complement(460..1104)
                     /gene="cheZ"
                     /locus_tag="OO327_003015"
     CDS             complement(460..1104)
                     /gene="cheZ"
                     /locus_tag="OO327_003015"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCR6019470.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLVQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1115..1504)
                     /gene="cheY"
                     /locus_tag="OO327_003016"
     CDS             complement(1115..1504)
                     /gene="cheY"
                     /locus_tag="OO327_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCR6019471.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1519..2568)
                     /gene="cheB"
                     /locus_tag="OO327_003017"
     CDS             complement(1519..2568)
                     /gene="cheB"
                     /locus_tag="OO327_003017"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCR6019472.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2571..3431)
                     /gene="cheR"
                     /locus_tag="OO327_003018"
     CDS             complement(2571..3431)
                     /gene="cheR"
                     /locus_tag="OO327_003018"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCR6019473.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3450..5051)
                     /gene="tap"
                     /locus_tag="OO327_003019"
     CDS             complement(3450..5051)
                     /gene="tap"
                     /locus_tag="OO327_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCR6019474.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5097..6758)
                     /gene="tar"
                     /locus_tag="OO327_003020"
     CDS             complement(5097..6758)
                     /gene="tar"
                     /locus_tag="OO327_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCR6019475.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6903..7406)
                     /gene="cheW"
                     /locus_tag="OO327_003021"
     CDS             complement(6903..7406)
                     /gene="cheW"
                     /locus_tag="OO327_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCR6019476.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7427..9391)
                     /gene="cheA"
                     /locus_tag="OO327_003022"
     CDS             complement(7427..9391)
                     /gene="cheA"
                     /locus_tag="OO327_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCR6019477.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9396..10322)
                     /gene="motB"
                     /locus_tag="OO327_003023"
     CDS             complement(9396..10322)
                     /gene="motB"
                     /locus_tag="OO327_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCR6019478.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10319..11206)
                     /gene="motA"
                     /locus_tag="OO327_003024"
     CDS             complement(10319..11206)
                     /gene="motA"
                     /locus_tag="OO327_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCR6019479.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11333..11911)
                     /gene="flhC"
                     /locus_tag="OO327_003025"
     CDS             complement(11333..11911)
                     /gene="flhC"
                     /locus_tag="OO327_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCR6019480.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11914..>12114)
                     /locus_tag="OO327_003026"
     CDS             complement(11914..>12114)
                     /locus_tag="OO327_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCR6019481.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         2784 a         3388 c         2934 g         3008 t
ORIGIN      
        1 aacactacgc taatgagttg ggatgccgtg ctctatgatc tacgtagtga aaaaattatc
       61 agtaaagata attacttgac cgacttaaac cgcagacatc ttgaacttgt tcttcatact
      121 aaccccaatc caagcggttc ggtaacgaag attttacgca ctatttttac taagcaggta
      181 aagagtttag ggccggaact ctgtcatttc ttgcaccatg aactgacgaa aaaacgttat
      241 gaagtatggc atcactacga tgtactgcac taccagtcat cgtcattttc tcgttcacaa
      301 tttaatgcat tgataaattt actggcgcaa caatcacgct tcgctgatct aaaattatca
      361 aactacaaat aaccatcctc cacgtactcc ctgaacgata cccgccaccc tgctatcggc
      421 ttggcatcat cgcgcaggcc gaagttcagg caatacaaat caaaatccaa gactatccaa
      481 caaatcgtcc acctgatcct gactggctac cacgccggct ttgctggtat cgacctgagg
      541 tccattaagc aaactctggt tttcacgttt tggacgcgac tcctgttccg ggatgttttc
      601 caacagcacc atcagcaact ggcgttcgat ctcctgaatg acatccatca tccgcttaat
      661 aacctgcccg gtgagatcct gaaaatcctg cgccatcatg atttccagca gttgcgcgtt
      721 agtaaagctg gtatgcgcgg gtacatctgc cagaaattgt cgtgtatctg ttaccagttc
      781 acgggcgtcg gcaaggtcaa tcggatcggc aaaccagtca tcccaacgtt gggttaacgc
      841 ttttgctgat ttctccattt gatcctgatg cggttgtgac gcctcaacac tgttcagcgc
      901 ccgctccgca gcctgggcgg tcatctgcac aacatagtac aaacgatcgc gcgcatcggg
      961 gatggcttcc gccgcttcgg caatggcctg aaccagcccc agttcccgca aactgtcgcg
     1021 cagcatatgc gtcaggctgc cgatgcgcgc aatgatatcg ccagctgaat gctcgtcagc
     1081 aggtttgatt gatggttgca tcatagtcgc atcctcacat gcccagtttc tcaaagattt
     1141 tgttgagttt ttcctccagc gtcgcggcgg taaatggctt caccacatag ccactggccc
     1201 ccgcttgcgc cgcagcaatg atgttctctt tcttcgcttc tgcagtcacc attaacactg
     1261 gcaatgccga catcgcgcca tccgcacgaa ttgttttcag caattccagg ccatccatat
     1321 tgggcatgtt ccagtcggag ataacaaatc cataaccgcc tgcctgcaac ttattgagag
     1381 cgtcgacacc atcttccgct tcctcaacat tattgaatcc cagctctttc agcaggttac
     1441 gcactatgcg tcgcatggtg gaaaagtcat ccacaaccaa aaatttaagt tctttatccg
     1501 ccatttcaca ctcctgattt aaatacgtat cgcctgtccg gcactaattg ttgccaacat
     1561 ttgctggctt acctggctaa gatcgaccac ttcgcagaca ccacccatat tgatggcctc
     1621 gcgcggcatg ccgaacacca cgcaacttgc ttcgttttgc gcaagggtcc atgcccccgc
     1681 ctgacgcatc gccaacattc ccgccgcgcc gtcgttgccc ataccggtca ggatcacccc
     1741 aaccgcatta cgccccgcct gtttggcgac agaatggaac aacacatcta ccgaaggccg
     1801 atgacggtta accgccgggc cgtcgtgaat tttgatttga taatttgcgc cactacgcgc
     1861 cagttccata tgccgatcgc ccggcgcaat ataggcatgc cccggcaaga cacgttctcc
     1921 gtcttcggct tctttaaccc cgatctggca aagcttatta agtctgtcgg caaaagagcg
     1981 ggtgaaaccg ggcggcatat gttgggtaat taacaatgcc gggctggaaa gcggcaacgg
     2041 ttgcagtacg tgacgaattg cctcagttcc acccgttgaa gcaccaatcg caatcagttt
     2101 ttcagaactc aacaacggcc ctgccttcag cgttgtcggt gctgacaatg gcttatgtgc
     2161 tgcaaggctc gcctttgctg ccgtacgcac cttttcagca atcatttcgc tatacgccag
     2221 cattccttcg cgaataccca gttgcggttt ggtgacaaaa tctattgccc ccagctccag
     2281 cgcgcgcagc gtgacttctg atcctttgcc ggtcaaggaa gaaaccatca caacgggcat
     2341 tggacgcaaa cgcattaatt tttcgaggaa atccagtccg tccatccgcg gcatttcaac
     2401 atccagcgtc agcacatcgg gattgaattt cttaatcaag tcacgcgcaa ccagcggatc
     2461 aggcgcggtc gccaccattt ccatgtcgct atggctgttg atgatttctg tcatgatctg
     2521 gcgcatcagt gccgaatcat cgacagataa caccctgatt ttgctcatcg ttaatcctta
     2581 cttagcgcat acaccgtctg accacgcagc gtgaagcggc gctcgaggtg gctaaagttt
     2641 tcagagtgac ccgcaaacag caatccgtcg ggtttaagga gcggaacaaa gcggcgcaaa
     2701 atctcctgct gggtagtttg atcgaagtag atcatgacgt tacgacagaa gatcgcatca
     2761 aacggccccg gcacggtgta ctgtttcgcc agtaaattca gcggggcaaa atcaacatag
     2821 ttcgccagct cctgacgcac gcgtaccagc ccttcatgcg gtcccgtccc tcgcatgaaa
     2881 taccgttgca gttgctgcgg cgtcaggttt ttcaactctt catggcgata gataccgctt
     2941 ctggcttttt ccagcacttc ggtgtcgata tcactggcaa acactttcca gcgtccgggc
     3001 acggtgccca atgtgtcagc cagagtcatc gcaatgctgt acggctcttc gccggtcgaa
     3061 gccgccgcgc tccatacgcg atcctcgcca gaacggcgac gtgcgtgatc cgcgagcaga
     3121 gggaaatgat gagcctcacg gaaaaatgcc gtcagattcg tggtcagcga attgataaac
     3181 gcctgccact caccgctgtg ctgattagat tccagcaagt tcagataatg accgaaatcc
     3241 gtcagtccca gagaacgcaa acgacgaacc agtcggttgt aaaccatgtc gcgtttatgg
     3301 tcagccagaa cgatcccggc tcgttgatag atcaattgac ttatccgccg aaaatgcgcg
     3361 tcggaaagcg ccaggcgctc ggtcatctgt aacaataaag acgtttgccc acagggcaga
     3421 gatgaagtca tagcgccttc tcaatcactt caggatacca ctggcgcaat ttgtaactgc
     3481 gccgactcat gtcgtgctac ttcatgttct tcaagggtaa ataccgccac acgcgacgaa
     3541 agatggtcgg cctgattcgc cagttgttcc gttgccaccg ccgcttcttc taccagcgag
     3601 gagttctgct gagtcacctg atccatctgg ctgacagcct gtgcaacctg ctcaatcccc
     3661 cgccgttgtt cttccgacgc cgaagcaatt tctcccataa tgtcgttcac gcgggtcacc
     3721 gaactgacaa tatcggtcat ggtcgcggcg gcgttattca ccagtttcga accctgctgg
     3781 acacgattga ctgactcttc gatcagccct ttgatctctt ttgctgcctg cgcgctacgg
     3841 ctggcaagat tgcgcacttc acctgccact accgcaaaac cacgcccctg ctctccggcg
     3901 cgagccgctt ccactgccgc attcagggcc agaatattgg tctggaaagc aattccgtcg
     3961 ataacgctga taatgtcgcc aattttttgc gagctggtgg cgatctcctg catggtgtga
     4021 gtcatggtac tgacctgaac accgcccgcc tgcgccgttg tcgcggcatt ttttgccagt
     4081 tccgacgcct gtcgtgcgtt atcggcgttt tgccctaccg tggcggttaa ttgctccata
     4141 ctggcggccg tttgtgccag cgatgccgcc tgctgttcgg tacgacttga gagatcgtta
     4201 ttgcctgcga caatctccgc gatcccaatg tgcatctcat ggcttccctt acgcacatca
     4261 ctcaccgtcc cacgcaaagc ctgctgcatg gtcttcaggc tggcaaaaat ggcggtgatc
     4321 tcattacgac catataccgc aatcggacgc gccagattac ccgcagcaat gctgtcaaaa
     4381 tggctaccga taatggccag tggttgaaca atcatcttgc gcgtccacca cagcgcactg
     4441 ctgatgtaga tcgccgcaac aataatcatg ctgataaaca ccagcgccga aatctgatag
     4501 ttacgctggc tttgcgcact ggcggcttcc agcacatggt tgatctccag ctgccaggcc
     4561 tcaaagttaa cgtcaaacgc attctgtgat ccctgcaccg gcgcagtgag gaaatccgaa
     4621 agttgattac tttcaagcca ggtcgcctga tgttcgagat cgttgtgcca gcgggcaaaa
     4681 cttttctccg tctctttttg caatgccctg acgtgctcgt tgcccgcagt catcgccata
     4741 aaacttttaa acagcgtggt ggattgcgtc agactggcgc gcgccgtcgt catcaacgtt
     4801 ttaatgtcat ccgccggata gctaagcgct gtcagagtgc ctgctttgtt cagcgcggta
     4861 ctggcctgta acattaccgc ccgagtttgc gctaatgccg cacgttgctg attgctctgc
     4921 tcgccgtgat tcagtcgttg caaatcgtcg cgaaatgccc aaaaagacat gccgttactg
     4981 ccaatctgca agatcccgca gagaatcaaa attaaaaaca gcgtggtcga aattcgaata
     5041 cgattaaaca tcaacgctcc ccatcaggcg gcaatgaccg cgttagtaaa aactcgtcaa
     5101 aatgtttccc agtttggatc ttgttcagta attcgcagtc gtggctgtgc cggtggttgc
     5161 tcactggcag gacgggatgg tgtttgcggt ttattggtga gtgggctggc tgccagacgg
     5221 aacgcggaaa ccgcttgcgt taaacgactc gcctgttctt ccagcgcagc tgcggcggca
     5281 gctgattcct gcaccagcga tgcgttctgt tgcgtgacgc gatccatttc cgaaaccgcc
     5341 aatgcgactt gatcgatgcc acggctctgt tcatccgatg ccgatgcaat ctcgcccata
     5401 atgtcagtca cgcgagtgac agcattgacg atattgttca ttgtttcccc ggcgctttcg
     5461 accagcaccg aaccggtatc aatgcgcgag acggagtctt caatgagggc tttgatctct
     5521 tttgccgcca gggcgctgcg actggcaaga ttacgcactt cacccgccac cacggcaaaa
     5581 ccacggccct gttcacccgc acgcgcggct tcaaccgcgg cattcagcgc gaggatatta
     5641 gtctggaagg caataccgtc gataacgctg ataatgtcgg caattttctt cgaactatcg
     5701 gcgatctcat gcatcgtttt cactacgcca tccaccactt tgccgccgtg ctgggcggtg
     5761 tcggaggcac tttgcgccag ttgcgaggcc tggcgggcgt tatcggcgtt ttgcttcact
     5821 gtcgcggtga gctgctccat gctggcggca gtttcttcca gcgcggatgc ctgctgttca
     5881 gtacgggagg aaagatcggt gttgcccgcc gcaatttcac gggtaccggc atagatggca
     5941 tctgaacctt cgcggacatg agtgacggtg tcagtcaaag agcgttgcat atgtgaaacg
     6001 ctctgcgcca ggtcacccat ttcactgcgc ccgtcaatgg tcagggtatt cgccaggtta
     6061 ccaccggcga tttcgcaaat gtgagcaata atttttgcca gcggagtaag caacatacgg
     6121 cgaatgccgt accacgccac cagcagaatc aataccacca ccagcgcgat aaccgccagt
     6181 tgccactggg caaatcggta atcatctgcg ttgtcagtga cgatatcgcg atacagtttt
     6241 tcactgctga gggcgtactg agcaaacgct tcgcccattg cattttgcat tccctgggtt
     6301 ggctgagcga aataagctcc agtattgcca taatcaagat aatcaatcag ttcagttaac
     6361 gctgtgtaat agtttttata tttttcatca aaattacggc tggtagcgac catttcaggt
     6421 aacggtgcca tgcttttgaa ttttttataa tgcgtcgcgg cctgggccaa tgttttcctg
     6481 gcgctatcga gcaattcaac tttggcgtta ctttgttgat tggaggaatc catcatcatc
     6541 cgcaccgctg aacgactcag gttaatgcgc gtttgcagca ttaaatccca ggttgacgtc
     6601 agctcgccct gctgttcccg taattgattg gaaaccacaa agctcttctg gctatggtga
     6661 agggaagaaa aaaacaaact gccggaaata agctgtaaca gtgcgaatac ccccagcacc
     6721 attaccaaca gcgtgactac gcggatacgg ttaatcataa ggcaccttcc tgaaaacaag
     6781 ttgatctcgt tatcggcaag aaggggggaa actttattgc tgatgccacc cgccgcgaaa
     6841 ttgaaataaa aaacccgatg cgcagatcat cgggttcatt tcaattgagg aaatcgggag
     6901 aattacgcca cttctgacgc cgcgctatct aacagcgcca tctcttcgct gttcagcagt
     6961 ttttcgatgt tcaccagaat caacatacgg tcgcccagtg cgcccagtcc agtgagatat
     7021 tctgttgaaa gcgtcacggc aaactctggt gccggacgaa tttgctccgc cgtcaatgaa
     7081 agcacgtctg aaacgccgtc aaccacgatg ccgaccaccc gctgtccgag attcaggacg
     7141 ataactaccg tgttgtcgtt atagtccaca tccacctggc tgaacttaat tcgtaagtca
     7201 acaatcggca caataacgcc gcgcagattc gtgacgcctt tgataaacgc tggcgtgttc
     7261 gcaatccgtg ttacctgatc gtagccacgg atctcctgca ctttcaggat atcaataccg
     7321 tactcttcat caccaagggt aaataccaga aattcctggc ctgacggctc gctggccagc
     7381 tttgttacat tcgtcatacc ggtcatattg ttaccttttt actcattcag gcggcggtgt
     7441 tcgccatacg ttgttcgcgg tttatcgcct gcaaggcgga gacatcaaca atcagtgcca
     7501 cgctgccgtc gccaagaatg gtcgcagcag aaatgccggg gactttgcga tagttacttt
     7561 caaggttttt aaccacaacc tggtgttgac caattaattg atccaccagc aaggcgtagc
     7621 ggcgaccgcc actttgtaag atcaccacaa ttccctgggt ggcttcggtt ttcgcgcccg
     7681 cgacgttgaa cactttccac agttcgacga tgggcagata ttcaccccgc acttccagca
     7741 cccgctcgcc gccggccagt ggatggagat cggcttcacg gggttgcagt gattccataa
     7801 cagcattcag cggcagaatg aaaacttcat ccgcaacgcg tacggacatg ccgtcgagga
     7861 tggccagcgt cagcggcagt aaaatgcgga tcgtagtgcc agtaccctgc ttcgactgga
     7921 tttcgacatg accgcccatc tcctggatat tacgtttaac gacgtccatg ccgacgccgc
     7981 gcccggagac gtcggtgacc tgctctgccg tggagaagcc tggtgcaaat atcagcattg
     8041 caacttcgtc gtcgctcatg ttttcgctga cagtcaaacc ttgcgaggcc gcttttgcca
     8101 gaattcgctc acggtttagc cccgccccat cgtcggtcac ttcaatgcaa atgttgccgc
     8161 cctgatgttc ggcagacaga attaaatttc cgacgctgtt tttacctgcg gcgagccgtt
     8221 tttctggcag ttcaataccg tgatcgaggc tattgcgtac caggtgggtc agcgggtcga
     8281 taatgcgttc tatcaggctc ttgtcgagct cggtggaact gcccaccagc gtcagttcta
     8341 cctgcttgcc gagttttccc gccagatcac gcaccagccg gggatagcga ctaaagacat
     8401 attccatcgg catcatgcga atcgacatca ctgattcctg caaatcacgg gcgttacgtt
     8461 gtaactgccc catgctggta atcaaatcac catgattaac cgggtccagt tcgctggaac
     8521 gctgggcaag catggactgg gtgataacca gctcgccgac gaggttaatt aattgatcaa
     8581 ccttttctac cgctacacgg atgctggtgg attcactgct gcgcgtcgtt ttttcccgct
     8641 ccacgcggcc ggttggcgct tgttcggctg ccagtttaag cacaggtggg gtagctattt
     8701 ttggcgaaac ttctactgtt tcaaaggtaa tctgatcggc ttcaatcaca aaacagagta
     8761 ccgctgtgat gtcatcttcg gcgatatcgc ccggtaatat tgccgagaga gaatccgccc
     8821 ctttcaccac gtcatttaac gttgtcagat gccccagctc ttcttccagc aggtcgactt
     8881 cgccggcctt caggcgcgaa aggataattc gtcgcggcaa ctgactgcga ctttgctcat
     8941 cttgcggttc acttttggca accacactta atcgggtcac tgcggatggc gtttcgcctt
     9001 tcgcttctaa tgccagttga cgcaaggcct ggcagatata atcgtagctg gcggcatccg
     9061 gctcttgcga ctgtttataa gcgtcgagct gttcttgcat gatgtccttc gtttccaaaa
     9121 acagattgat aatgtcggtg ttgagttgca tctcacctcg tctggcttca tcgagcaggt
     9181 tttccatcag atgcgtggtt tcctgcaaaa cgctgaagcc aaaagttcct gcccctcctt
     9241 tgatcgagtg ggcagcccga aagatggcat tcaattgttc ggcatctggc gcttccggct
     9301 gcaaaaccag cagatgctgc tccatatcag ccaacagttc gtccgcttca tcaaaaaatg
     9361 tctgataaaa atcgcttata tcgatgctca cgctgtcacc tcggttcggc tgatggcatt
     9421 gtgggaacac tgacctgtgg tgcaacctca ggtttttcta gggcgcttac tggctcattc
     9481 tggctttcgg cgttttcatg caaaatggcc tgttcggctt gtttgttcag taccaacagg
     9541 ctgatgcgac ggttgacggc atcatcaggt ccgcgatcgc ttaagcgcat cgttgccgcc
     9601 atgccgacga cacgtaacac tttgccgcta tccaaccctc cgaccatcag ttcgcggcgg
     9661 gatgcattgg cccgatcggc agaaagctcc cagttgctgt aacctttctc accgctggcg
     9721 tagggaaaat catcggtatg acctgaaagg ctaatacggt tgggaatacc gttcagcaca
     9781 ggcgcgatgg cgcgcagaat gtcgcgcata tagggttcga catcggcact gccggttcta
     9841 aacatcgggc gattctggct atcgatgatc tgaatacgta gaccttcctg gaccagatcg
     9901 attttgagat ggagacgtaa cgcccgcagt ttcggatcgg actctatcaa ctggtcgaga
     9961 tcaccccgca atttccgcaa tcgactttgc tccatgcgtt ttttcagctc ttcgatgttc
    10021 ggctgcttat tcacttcccc ctggctttgg gtgtaatcat caccaccgcc gggaattggg
    10081 ctttcactat tggaaatgcg atcgccgccc gtaaccgctg tcgccagtgg agtccggaag
    10141 tactccgcaa tctgaatcaa ctcttttggg ctggagatgg agatcagcca catcaccaga
    10201 aaaaaggcca tcatcgcagt cataaagtcg gcataagcaa tcttccacga tccatgtgct
    10261 gccccgtggc ttttggcttt gcgtcgtttg acgacaataa tcggatgcgc ttgattcttc
    10321 atgcttcctc ggttgtcgtc tgttgttgcg gatttttcac cgcacgcaca tgctcttcca
    10381 gttcaataaa cgacggacgt tcgctggaat agagcgtttt gcgaccaaac tcaacggcga
    10441 taggcggtgc gtaaccgttc agattagaaa gcagagtgac tttgacgcac tgcatcattt
    10501 tgctggtttc ggcgcttttc tgacgtaaaa cactcgctag tggggaaata aatccgtaag
    10561 ccaataaaat accgaggaaa gtccccacca tcgcatgtgc gataagcgcc cccagctcgg
    10621 cggcaggacg atcggctgaa cctaacgcgt gaacgccccc cattacagcc gcgacaatac
    10681 caaacgccgg aagtgagtcc ccgaccagcg ccagactgtt cgccgggact tctgcctcgc
    10741 tttcgtgcgt ctcaatctct tcatccatca gggcttcgat ttcgaaggtg ttcatgtgac
    10801 cgctgataat caggcgcaga taatcgacga taaaatcaag catgacgcta tccgcgagga
    10861 tgcgtgggta gctggcgaag atctcgctct cacggggatt ttcaatatca cgttccagcg
    10921 aaaacatccc catctgccgc gatttcgcca tcaaccgata aagcagagcc agcagatcca
    10981 tatacattgc tttggtgtat ttggagcgac gaaacagcaa cggcagcgct ttcagcgtgc
    11041 ctttaatcgc tttgccatta ttgccgacga taaacgaccc aatccctgca ccggcaataa
    11101 tcaccagttc agcgggttga tagagtgctc caaggcttcc accggtcatc aaataaccgc
    11161 cgaaaactgt accgagaaca accaggtaac ctaataagat aagcacgaca tcatccttcc
    11221 actgttgacc atgacaggat gttcagtcgt caggcgttaa cgcgcgattg gggaaaaaaa
    11281 aagcagcggt acgtcgttac cgctgctgga atgttgcgcc acaccgtatc agttaaacag
    11341 cctgtactct ctgttcatcc agcagttgtg ggataatatc ggcaggattc tgggaaagtt
    11401 tacgtctttt tactgcccgg gatggcggtt gacataagct gcaggcaaag ctgccaacag
    11461 gctggtgagc gtgggtaata aaattgccgc cgcagcagtt gcagctggaa agttgcagta
    11521 atccactttc aacaaaccgc accaatgtcc aggcacgggt taatgccagc agtggtcctt
    11581 cttctgcttg tgggcactgt tcaaggtata aacggtaggc tttgatcacc gcatcgacgc
    11641 cattacacaa accggttttc agtaaaaact gccatgcatt acagaacatc gaagcatgaa
    11701 cgttttgttc ccaggtcata aaccagtcgg ttgagaatgg cagcatgcct ttcggcggtg
    11761 ggcttccgcg cagttcttta taaagtttta tcaggcgtcc gcgacttaac tgtgtttcgc
    11821 tttccagcat ctgcaaacga gcgcccaggg tgatcaattc cattgccagc tgaatatccc
    11881 gcgcttcctg aacaatgctt ttttcactca tgatcaggcc cttttcttgc gcagcgcttc
    11941 ttcaggctga ttaacatcat tcagcaagcg tgttgagagc atgatgccgg tatgaatttg
    12001 ctggagatcg tcaacgcggg aatcttgcgt caactgagta atcgtctggt ggctgtcaaa
    12061 acggaagtga caaaccagct gattggtttc tgccagctta accatttgcg gaag
//
LOCUS       DAJTSI010000121        12101 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000121 DAJTSI010000000
VERSION     DAJTSI010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12101
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.121"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..116)
                     /locus_tag="OO327_003027"
     CDS             complement(<1..116)
                     /locus_tag="OO327_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6019482.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQV"
     gene            492..2132
                     /gene="trg"
                     /locus_tag="OO327_003028"
     CDS             492..2132
                     /gene="trg"
                     /locus_tag="OO327_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCR6019483.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2170..3093)
                     /locus_tag="OO327_003029"
     CDS             complement(2170..3093)
                     /locus_tag="OO327_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6019484.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3310..4653
                     /locus_tag="OO327_003030"
     CDS             3310..4653
                     /locus_tag="OO327_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6019485.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4878..6533
                     /gene="opgD"
                     /locus_tag="OO327_003031"
                     /gene_synonym="mdoD"
     CDS             4878..6533
                     /gene="opgD"
                     /locus_tag="OO327_003031"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR6019486.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6673..6897
                     /locus_tag="OO327_003032"
     CDS             6673..6897
                     /locus_tag="OO327_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR6019487.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6960..7496
                     /gene="rimL"
                     /locus_tag="OO327_003033"
     CDS             6960..7496
                     /gene="rimL"
                     /locus_tag="OO327_003033"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR6019488.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQTLIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7491..8471)
                     /locus_tag="OO327_003034"
     CDS             complement(7491..8471)
                     /locus_tag="OO327_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019489.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8595..9587
                     /gene="tehA"
                     /locus_tag="OO327_003035"
     CDS             8595..9587
                     /gene="tehA"
                     /locus_tag="OO327_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR6019490.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9584..10177
                     /gene="tehB"
                     /locus_tag="OO327_003036"
     CDS             9584..10177
                     /gene="tehB"
                     /locus_tag="OO327_003036"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR6019491.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10480..11136
                     /locus_tag="OO327_003037"
     CDS             10480..11136
                     /locus_tag="OO327_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCR6019492.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYNNYSDLKETTSAT
                     GKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIA
                     QRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRL
                     YFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK
                     "
     gene            complement(11154..11660)
                     /gene="tnpA"
                     /locus_tag="OO327_003038"
                     /pseudo
     CDS             complement(11154..11660)
                     /gene="tnpA"
                     /locus_tag="OO327_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            11668..12101
                     /locus_tag="OO327_003039"
                     /pseudo
     CDS             11668..12101
                     /locus_tag="OO327_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         3138 a         2824 c         3037 g         3102 t
ORIGIN      
        1 acctgggttc gggggacacc aaaacgtgcc gagatgatcc tgtaaccatc atcagtagtg
       61 aagtagtgat tcacgacttc aaggcgcttt tcaaaagggt attttggctt tgacatatta
      121 ggggctattc catttcatcg tccaacaaaa tgggtgcagt acagttgata gaaatatcgg
      181 tcggggcctt cgtctttctg attcccggtt agcctgaaaa cagaaagtct caggcacccg
      241 caggcatcct atgaggtttc cttagggacg aaaataatca cttcacgaaa ttgcgtgctg
      301 ttttccagaa tttttcgtca ttcgggttag ccagtttagc cattcgttac tctcttcatt
      361 ccaatagcat taattttctc tgcaataatt gttgtaaaaa tgtgacgcaa agaggttttt
      421 ggtcataagt aattaccgtc aagtgccgat gactttctat caggagtaaa cctggacgag
      481 agacaacggt aatgaataca actccctcac agcgattagg ttttttgcat cacatcaggc
      541 tggttccgtt atttgcctgc attcttggcg gtatcttagt tctattcgca ttaagttctg
      601 ctctggctgg ctatttcctc tggcaggccg atcgcgatca gcgtgatgtt actgcggaga
      661 ttgagattcg gaccgggtta gcgaacagtt cagatttttt gcgttcagcc cggatcaata
      721 tgattcaggc cggggctgcg agtcgtattg cggaaatgga agcaatgaag cgaaatattg
      781 cgcaagccga atcggagatt aaacagtcgc agcaaggtta tcgtgcttat cagaatcgac
      841 cggtgaaaac acctgctgat gaagccttcg acactgaatt aaatcaacgc tttcaggctt
      901 atatcacggg tatgcaacct atgttgaaat atgccaaaaa tggcatgttt gaagcgatta
      961 tcaatcatga aagtgagcag atccgaccgc tggataatgc ttataccgat attttgaaca
     1021 aagccgttaa gatacgtagc accagagcca accaactggc ggaactggcc catcagcgca
     1081 cccgcctggg tgggatgttc atgattggcg cgtttgtgct tgccctggtc atgacgctga
     1141 taacatttat ggtgctacgt cggatcgtca ttcgtccact gcaacatgcc gcacaacgga
     1201 ttgaaaaaat cgccagtggc gatctgacga tgaatgatga accggcgggt cgtaatgaaa
     1261 tcggtcgctt aagtcgtcat ttacagcaaa tgcagcattc actggggatg acagtaggga
     1321 ctgttcgaca gggcgcggaa gagatttatc gtggcaccag cgaaatttca gctggcaatg
     1381 cggacctgtc atctcgcacc gaagaacaag cggcggctat cgaacaaact gccgccagca
     1441 tggagcaact cactgcgacg gtgaaacaga atgcggataa cgcgcatcat gccagcaaac
     1501 tggcgcaaga ggcttctatt aaagccagcg atggcgggca gacggtttcc ggtgtagtaa
     1561 aaacgatggg cgctatctcc acgagttcga agaaaatttc tgagatcacc gccgtcatca
     1621 acagtattgc tttccagacg aatattctgg cactgaatgc tgccgttgaa gccgcgcgag
     1681 cgggtgagca agggcgtgga tttgccgttg tcgccagcga agtacggaca ctcgcaagcc
     1741 gcagcgctca ggcagcgaaa gagattgaag gcttgatcag tgaatcagtc aggttaattg
     1801 acctggggtc ggatgaggtg gcaacggcag ggaaaaccat gagcactatt gttgatgccg
     1861 tcgcgagtgt cacacatatc atgcaggaaa tcgccgccgc ctcggatgaa caaagtagag
     1921 gcataactca ggttagccag gcgatttctg aaatggataa ggtgacgcaa cagaatgctt
     1981 ctctggtaga agaggcctca gcagcggcgg tgtcccttga agaacaggct gcacgattaa
     2041 ctgaggcggt gaacgtattc cgtctgaaca aacattctgt gtcggcagaa cctcgcggag
     2101 cgggtgaacc agttagtttc gctacggtgt gaaaatgttc aaggagggat cgacagatcc
     2161 cttcaccttt cagaacggca ttgattttcg aatagcgtta atcatcaact ggcaaccaga
     2221 agagaacgtc gcatctacgc gggtcagtat tccaatcggt tcgcctgcac catgtcccgg
     2281 aacaggcagg gccaccagcg tggcatgacg caggtcgtct tttacagcgc cagaagggac
     2341 aaaccacacg taatcgtatt caacggtaag ttgacgagat agcgaagtag acagcgtttc
     2401 gatacaaccc gaaggaattt tacagccctg gctctgcact aatgcatctg aatgctggcg
     2461 tggcgcagtg ccttctggtg atacaacgac cggccattcc agcacccggc ttagcgttac
     2521 gttctcctga agtagcgggt gattagggcg gacaaccagc ttcaacgatt caagaaacag
     2581 cagttcgtaa ttaagcccgg tcatcagttc aggatctgac atccgaccaa tgccgatatc
     2641 gatttccccg gtttttaaac ccgccagaat catagggtta ctcattgtcg caacttgcaa
     2701 ggtcgtctct ttttgttgct gatgaaactg acctataacc gaaggtaata tccccagtgc
     2761 cgcagtaggt agtgcaccaa ccctgacgac atcattatta agaccttctt tacgatgaag
     2821 cgactgtccg gcagtgttga tggcgtcaag aactctgact gcatgcgtta aaaattgttc
     2881 gccgggtaag gtaagttgcg ccccctgacg accacgctca aacaagcgag cgcccgtcag
     2941 ctgctccagt tcattcaatg tattagagag cgcaggttga ctcaaattaa gggtttcagc
     3001 tgcgcgcccc aaagttcctt gttgtgcgac agctacgaat gtatgaaggt ggcgcaaacg
     3061 gatgcgctga ctaaacagac tatttttttc cataagcgat gttaaaaacg aagcggtgtc
     3121 gctgacaagt gaagttgttt gattatgata acttgattgc aaaatattat taacaattaa
     3181 agcaattatg ttacagcaaa atggataata ttgatgtttt cgcggcgaga tcacagtttg
     3241 taaattcttc ccgccagagt gaatgcggtt acctacactc cagattactg accactggag
     3301 gcagacacta tggcgaacag catcacggcg gatgagattc gggaacagtt ttcgcaggca
     3361 atgtcagcca tgtaccagca agaagttccg caatatggca cgctgctgga actggtagct
     3421 gatgtgaatc tggctgtgct ggaaaacaat cctcaactgc acgaaaaaat ggtaaatgca
     3481 gacgagctgg cgcgactgaa tgttgaacgt catggggcga ttcgcgttgg gactgcacaa
     3541 gagcttgcta ctcttcggcg gatgtttgcc attatgggga tgtacccggt gagctattac
     3601 gatctctcgc aggcaggggt gccggtacat tcgacagcat ttcggcccat tgatgatgct
     3661 tctctggcgc gtaatccctt ccgcgttttt acatccttac tccgccttga gcttatcgag
     3721 aacgaaattt tgcgccagaa agcggcggag attctgcgtc agcgcgatat cttcacccca
     3781 cgttgtcgac aactgttaga ggaatatgag cagcagggcg gttttaacga aacacaggca
     3841 caggagtttg tgcaggaagc cctggaaacg tttcgctggc accagttagc aacggtagat
     3901 gaagaaacct atcgcgcctt gcacaacgaa catcggttga ttgctgatgt ggtctgtttt
     3961 cctggatgcc atatcaacca cctgacgcca cgtacgctgg atattgaccg ggtgcagtcg
     4021 atgatgcctg aatgcggaat tgaacccaaa attctcatcg aggggccgcc gcgccgcgag
     4081 gtaccgattt tactacgcca gaccagcttt aaagcactgg aagagacggt gttgtttgcg
     4141 gggcagaaac agggcacgca taccgcgcgc tttggtgaaa ttgagcagcg tggcgtggca
     4201 ttaacgccga aagggcgaca actgtatgat gatcttctgc gtaacgctgg aaccgggcag
     4261 gataatctca ctcaccaaat gcatttacag gaaaccttcc gcacttttcc tgacagtgag
     4321 tttttaatgc gtcagcaagg gttggcatgg ttccggtacc gtctgacgcc ttcgggtgag
     4381 gcgcatcgtc aggcgattca tcctggagac gatccacagc ccttaattga acgtggttgg
     4441 gtagtggcgc aacccatcac ctatgaagat ttcttgcccg ttagcgcggc ggggatcttc
     4501 cagtcaaatc tgggtaatga aacgcagaca cgcagtcacg gtaatgccag tcgcgaagca
     4561 tttgagcagg cgttgggttg tccggttttg gatgagttcc agctttacca ggaagcggaa
     4621 gaacgcagta aacgtcgctg tggtttgctt taaattctga ccatccgcct ttgcaaaaat
     4681 ttgcctgatt ttacaaacga atcaggctca tcccatcgac ataaaaaaaa tgccgattta
     4741 tgcatattct ctcagttcaa caattggatt attaataaat attgtctaga gtgagcggtc
     4801 ataaataagc actttcttgc cgctgaaaac gaccagcgcg ggaccattca caacaccaga
     4861 aggactcact ttcaggtatg gatcgtagac gatttattaa aggttcaatg gctatggccg
     4921 ccgtgtgcgg taccagcggc attgcttctc ttttttctca ggcagcattc gcggcagatt
     4981 ctgatattgc cgacgggcaa acccagcgtt ttgacttctc cattctacag tcaatggcgc
     5041 acgacttagc gcaaacagcg tggcgtggtg cgcctcgtcc gttacctgac acgctggcga
     5101 caatgacgcc gcaggcttat aacagtattc aatacgacgc cgaaaaatcg ctctggcata
     5161 acgttgagaa ccgtcaactg gacgctcagt tcttccatat gggaatggga ttccgtcgcc
     5221 gcgttcgtat gttttctgta gatccagcaa cacatctggc gcgtgaaatt cactttcgcc
     5281 cggagttgtt caaatacaac gatgcaggtg ttgataccaa acaattagaa gggcaaagcg
     5341 atctcggctt tgccggtttt cgcgtgttta aagcccccga actggcgcgc cgtgatgtag
     5401 tatcatttct cggcgcgagt tatttccgcg ccgttgatga tacatatcaa tacggtttgt
     5461 cggcccgcgg cctggcgatc gacacttaca ccgacagtaa agaagagttc cccgacttta
     5521 ccgccttctg gtttgatacg gtaaaaccgg gggcaactac ctttaccgtt tatgcgttgc
     5581 tcgatagcgc cagcattact ggtgcctata agttcactat ccattgtgag aaaagtcagg
     5641 tgattatgga tgtggaaaat cacctgtatg cgcgcaaaga cattaaacag ctgggcattt
     5701 cgccgatgac cagtatgttc agctgcggta ctaatgaacg tcggatgtgc gatacaattc
     5761 atccgcaaat tcatgactct gatcgtctgt ccatgtggcg gggcaacggc gagtggattt
     5821 gccgtccgct gaataatccg caaaaattgc agttcaatgc ttacaccgac aacaacccga
     5881 aagggtttgg tttattgcaa ctggatcgtg acttctccca ttatcaggac attatgggct
     5941 ggtataacaa acgcccaagt ctgtgggtgg aaccgcgtaa caagtggggt aagggcacca
     6001 tcggcctgat ggaaatccca acaacgggcg aaacgctgga taacattgtc tgcttctggc
     6061 agccagaaaa agctgtaaaa gcaggtgatg agtttgcatt ccagtatcgt ctgtactgga
     6121 gtgcgcaacc gcctgttcat tgcccattag cgcgcgttat ggcgacgcgt accggcatgg
     6181 gcggtttccc ggaaggttgg gcgccaggtg aacactatcc cgaaaaatgg gcgcgtcgtt
     6241 ttgccgtcga tttcgttggt ggtgatctga aagctgccgc gccaaaaggc attgagccgg
     6301 tgattacgct ttccagtggg gaagcgaagc aaatcgaaat tctctatatt gaacccatcg
     6361 atggttatcg tattcagttt gactggtatc cgacttcgga ctccactgat ccggtcgata
     6421 tgcggatgta tctacgttgt cagggggacg ctatcagtga aacatggctg tatcagtatt
     6481 tcccgccagc gccggataaa cgtcagtatg ttgacgaccg cgtgatgagt taatcgtttt
     6541 ttcttcggca ccttcttcgg gaggtgccgt ctggttaaac acgatcccgc tcgcattttt
     6601 ccctaagtta aatgagtaat ctgatggtgt gtatttcaga tacaccgtgt cagccactaa
     6661 cagggagtgc gtatgtttcc agaataccga gatttaatat cccgtctgaa aaacgaaaat
     6721 cctcgcttta tgtccttgtt cgataaacac aataaacttg atcatgaaat tgccagaaag
     6781 gaaggttccg acggtcgagg gtacaatgcg gaagtggtcc gcatgaaaaa acaaaagcta
     6841 cagttaaaag atgagatgct caaaatcctg cagcaggaga gcgtcaaaga ggtgtaaact
     6901 ttcctaagcc gccagccagg cggctttttt aacaacagca tggattgact ggagataaga
     6961 tgactgaaac gataaaagta agcgaatcac ttgaattaca tgctgttgcg gaaagtcacg
     7021 tcacacctct ttatcagtta atctgtaaaa ataaaacctg gttacagcag tcgctaaact
     7081 ggccgcagtt cgttcagagt gaagaggaca cgcgaaaaac ggtgcagggt aatgtgatgt
     7141 tgcatcaacg cggctatgcc aaaatgttca tgattttcaa agaagatgaa cttatcggcg
     7201 ttatctcgtt taatcgtatt gaaccactga ataaaactgc tgaaatcggc tactggctgg
     7261 acgaatctca tcaggggcag gggatcattt ctcaggcgct gcagacattg attcatcatt
     7321 acgctcagtc tggtgaactt agacgcttcg tgatcaaatg tcgggtggac aatccgcaaa
     7381 gcaaccaggt cgctttgcgc aatggtttta tccttgaagg ttgcctgaaa caggctgagt
     7441 tcctgaatga tgcctatgat gatgtgaact tatacgcgcg tattatcgat tcataataac
     7501 cctgccagcg gcgttcgcgt aatgcgctcc tcgccgttaa tgacttttgg accacggaca
     7561 tgaaccgtgt cgccatcaaa ggcttcaacg acggcgtggt cggttaactc cacatggttt
     7621 tcaattatca ccgcacctgt aatacggctc tcaccttgta ttacaacgtg ctcgtcgagc
     7681 agaatcggtc caccacgtac cacggcattt ccgccaatca agacatgatg ttttaacaca
     7741 caattacctt ccacaatggc atattccgcc acctgcgaac tgtaatgaat cgtcggaatg
     7801 gcatcttctt ctatgccagc tttcacctgc gcgtgaccgt agactttagc gcaatcgcat
     7861 agccagacgt tgttctcttc attaccttca acactggcaa aatcaaaaac ttcggcgcga
     7921 tgttcaataa aagcataccg gacgacggca tcgccataaa tttgtgcctg gtggacaata
     7981 cgcgaggcgc taacccttgc gcgatcataa atttggagca ggagttgatg gtcgggcgtt
     8041 aagccttgtg cggcgacgat catagaatgt tgatcaatta aggcgtgacc aaatattcta
     8101 cattgcacgt aaactaatga gtcatgaatg gtgacactgt cacttatata tgcaccctgg
     8161 ctgatttcac tattatcgat ccagacgtta tccgttgcgt aaacctctcc ccacagcacg
     8221 ctggtgccgg tgatacgcgt attaccggaa atcaccgttc cgccgaacgc aatagcattc
     8281 tggtcgtaaa tccagcaatt tccttcctgt gctaacaccg tttcgcgatc gatccagccg
     8341 ccagcggttc ctgctatcac atcgttaaaa tcgctaatgg cgataatctg ccgtaataac
     8401 acactttttt tagtgccatc ttcctgataa ctaaaggctc gctgttcttc acttaggcga
     8461 tatttgcgca tagcgttttc ccacaggtga cttactataa ccgtagcaaa ttctgcggct
     8521 ctggctatgc tcgagaaaat tccataaaat gcatttcaaa tatactttat aaattaaaca
     8581 aaatgagtaa gaagatgcag agcgataaag tgctcaattt gccggcaggc tactttggta
     8641 ttgtgttggg gacgataggg atgggatttg cctggcgcta tgccagccag gtttggcagg
     8701 tcagccactg gttaggggat gggctggtga ttctggcgat gatcatctgg ggattattga
     8761 ctagcgcatt tattacccga ctcatacgct ttccgcatag cgtgctggcg gaagttcgcc
     8821 atccagtgct gagcagtttt gtgagtttgt ttccggcaac gacgatgctg gtggcgattg
     8881 gttttgttcc gtggtttcgc ccactggcgg tgtgcatgtt cagttttggt gtcgtggttc
     8941 agttggctta tgccgcctgg caaactgcgg gattatggcg cggatctcac cctgaagaag
     9001 ctaccacgcc tggactgtat ctgccgacag ttgccaacaa ctttatcagc gcaatggcct
     9061 gtggtgcgtt gggctacacc gacgccggtc tggtgttttt aggcgcaggc gttttctcat
     9121 ggctaagcct tgaaccggtg atcttgcagc gtctgcgcag ttcgggagaa ttacccacgg
     9181 cactgcggac atcactcggc attcagctcg ctcctgcgct ggtggcctgt agtgcctggc
     9241 tgagcgtcaa cggcggcgag ggtgacacgc tggcgaaaat gcttttcggt tatggactgc
     9301 tgcaactgct gtttatgcta cgtctgatgc catggtatct ctcccagcca tttaatgctt
     9361 cattctggag tttctcgttc ggcgtatctg cactggcaac caccggtttg catctgggga
     9421 gtggcagcga taatggattt ttccatacgc tggcggtgcc gctgtttatc tttaccaatt
     9481 ttattattgc aatactgctc atccgtactt ttgcgcttct gatgcaggga aaattgttag
     9541 tcagaaccga gcgcgccgtt ttaatgaaag cagaggacaa agaatgatca ttcgtgacga
     9601 aaactatttt actgataaat atgaattaac ccgcacacac tctgaagtac tggaagcggt
     9661 gaaagtggtt aaaccgggta aaacgctgga tctgggctgt ggcaatggtc gtaacagtct
     9721 ttacctggca gccaatggtt atgatgttga cgcatgggat aaaaatgcca tgagtatcgc
     9781 caacgtcgag cgcattaaat ccattgaaaa tctggataat ttacacaccc gggtcgttga
     9841 tctgaataac ctcacatttg atggacagta cgattttatt ctttcgactg tggtgctgat
     9901 gttccttgag gctaaaacca tcccagggtt gattgccaat atgcaacgtt gcactaaacc
     9961 tggtggttac aacctgattg tggcggcgat ggataccgct gattatccat gtaccgtcgg
    10021 cttcacgttt gccttcaaag agggagaatt acgtcgatat tacgaaggct gggagagggt
    10081 gaaatacaat gaagacgtcg gcgagctgca ccgcaccgac gccaacggta atcgtattaa
    10141 actgcgtttc gccacgatgc tggcacgtaa aaaatgaccc ggtaagcaca aaacgcgtga
    10201 aaattcccca cgctgagatg atttactgtt cttcttttcg gtaagcatat tttttatcga
    10261 agggatgtga aattaatcac agtagtcgaa gttttttagc agcttaactt actgaaattt
    10321 aactactgat gattgactta gccccttttt cggcattgac tatgtcgtct gaaaaggggc
    10381 tgaaaaattt attttcacca acactttttt tgccacaaca cgaagcggcg ctttttgcta
    10441 taacttagaa agtaatataa tcatctcagg aaactattca tgcgtaccac atcatttgcg
    10501 aaagttgcag ctttatgcgg cttgttggct ctgtctggtt gtgcctctaa aatcacccag
    10561 ccagataaat ataacaatta ctctgattta aaagaaacaa cctcagcaac aggtaaacct
    10621 gttttacgtt gggtagaccc gagttttgat caaagcaaat atgacagcat cgtctggaac
    10681 ccaatcactt attatccggt accaaaacct tctacccagg tagggcagaa agttctggat
    10741 aaaattttga actataccaa caccgaaatg aaagaagcga tagcgcagcg taaaccagtg
    10801 gttaccaccg ctgggccgcg tagtctgatt ttccgtgggg ccattaccgg tgtagatacc
    10861 agcaaagaag ggctgcaatt ctatgaagtg gttcctgttg cattagtggt tgcggggacg
    10921 caaatggcta caggccaccg taccatggat actcgcctct attttgaagg tgagctgatt
    10981 gatgcagcga ctaataaacc ggttatcaaa gtcgttcgtc agggcgaagg taaagacctg
    11041 aataacgaaa gtacgccaat ggctttcgaa aatattaaac aagttattga tgacatggcg
    11101 accgatgcca ccatgtttga cgttaacaaa aagtagttcc agacgcgcca tcgttagatg
    11161 gcgcttttta tccggtgcgc cgtaaaaccc catccttcag ggcggggata taaggcgcgg
    11221 ttttccacct gaccaggtgt ttgctgttgt tcaatgtatt ggcggatgac ggatattggc
    11281 gcacctccgc agctactggc aaaatagcca ggactccaca aaacgccttt gtagtaatac
    11341 ctgactgcaa tatctggtcg atctcgtcgc agtaacctac ccgatacgcc tttgaggctg
    11401 tttaccagac tggatatcgc cagtttggga ggttagttaa ttaacaaatg gacgtgatct
    11461 ggttcgccat ccatttcaac cagttcagct tcaaaataag cacatacatt tgaaaagtaa
    11521 gtgcgtagtt tttctgtcgc gtcatggtca aaaatctggc gtcggtatct ggtgacaaag
    11581 actaggtgaa catgcatcag gaaaacacaa tgccttccac gccggatatc ggtttctttt
    11641 ttcacagacc aaagtatcat ttgacctgtg aaacgattac aggcatttaa attccagtta
    11701 agaccaggtg gtcaacagga gtgtgaaatg aggcgcttcg caggcgcatg tcgtttcgtt
    11761 ttcaatcgtg ctctggcacg tcagaatgag aatcataagg ccggtaataa atacatccct
    11821 tacgggaaaa tggcttcctg gctggttgag tggaaaaatg ccactgaaac gcaatggctt
    11881 aaagattctc cctcacagcc attgcaacag tcactgaaag accttgagcg ggcttacaaa
    11941 aacttcttcc ggaagcgggc tgcttttccc cgattcaaaa agcggtgaca gaatgatgca
    12001 ttccgctacc cgcagggcgt taagctcgat caggaaaaca gccgtatttt tctgccgaaa
    12061 ctgggctgga tgcgctaccg gaatagccgt caggtcacgg g
//
LOCUS       DAJTSI010000122        12062 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000122 DAJTSI010000000
VERSION     DAJTSI010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12062
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.122"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(4..380)
                     /gene="rnpB"
                     /locus_tag="OO327_003040"
     ncRNA           complement(4..380)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO327_003040"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(413..1558)
                     /gene="garK"
                     /locus_tag="OO327_003041"
     CDS             complement(413..1558)
                     /gene="garK"
                     /locus_tag="OO327_003041"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR6019493.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(1655..2545)
                     /gene="garR"
                     /locus_tag="OO327_003042"
     CDS             complement(1655..2545)
                     /gene="garR"
                     /locus_tag="OO327_003042"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR6019494.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALVCYYEKLAKVEVTR"
     gene            complement(2575..3345)
                     /gene="garL"
                     /locus_tag="OO327_003043"
                     /pseudo
     CDS             complement(2575..3345)
                     /gene="garL"
                     /locus_tag="OO327_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(3361..4695)
                     /gene="garP"
                     /locus_tag="OO327_003044"
     CDS             complement(3361..4695)
                     /gene="garP"
                     /locus_tag="OO327_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR6019495.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5070..6641
                     /gene="garD"
                     /locus_tag="OO327_003045"
     CDS             5070..6641
                     /gene="garD"
                     /locus_tag="OO327_003045"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCR6019496.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMAPRTELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="OO327_003046"
     CDS             complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="OO327_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCR6019497.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLSRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            7766..9046
                     /gene="kbaZ"
                     /locus_tag="OO327_003047"
     CDS             7766..9046
                     /gene="kbaZ"
                     /locus_tag="OO327_003047"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HCR6019498.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            9069..9542
                     /gene="agaV"
                     /locus_tag="OO327_003048"
     CDS             9069..9542
                     /gene="agaV"
                     /locus_tag="OO327_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCR6019499.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            9553..10332
                     /locus_tag="OO327_003049"
     CDS             9553..10332
                     /locus_tag="OO327_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCR6019500.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            10322..11131
                     /locus_tag="OO327_003050"
     CDS             10322..11131
                     /locus_tag="OO327_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR6019501.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            11218..11652
                     /locus_tag="OO327_003051"
     CDS             11218..11652
                     /locus_tag="OO327_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR6019502.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            11649..>12062
                     /locus_tag="OO327_003052"
     CDS             11649..>12062
                     /locus_tag="OO327_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR6019503.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ERLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         2879 a         3252 c         3077 g         2854 t
ORIGIN      
        1 aacaggtgaa actgaccgat aagccgggtt ctgtcgtgga cagtcattca tctaggccag
       61 caatcgctca ctggctcaag cagcctaccc gggttcagta cgggccgtac cttatgaacc
      121 cctatttggc cttgctccgg gtggagttta ccgtgccacg gactgttacc agccgcgcgg
      181 tgcgctctta ccgcaccctt tcacccttac ctgatcccgc ttgcacgggc catcggcggt
      241 ttgctctctg ttgcactggt cgtgggtttc ccccccaggc gttacctggc accctgccct
      301 atggagcccg gactttcctc ccctccaccc gtctcccccg aagaggacga cgacgaagcg
      361 gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt tgcgcgccct tgtcaccccg
      421 tgttgcgcat tccaatcgcc agtgtcgcgg caatattacg tgaagcacgg cagatattgt
      481 cataagcccc gcggaatgct tcgtccaacg tacctatgct ggtcaatacg ctgaagaccg
      541 catcaatgcc atgctgatgt acaacgccaa catcatcggt caggctaccc gcaatgccaa
      601 tcaccggttt atggtacttc ttcgccacgt ttgcgacgcc aatcggtacc ttgccgtgaa
      661 tactctggct gtcaatacgc ccttcaccgg tgatcaccag cgtacaatcg tgaatatgtt
      721 cctccagatt cagcgccgta gtgacgattt caataccact tttcagttcc gcaccaagaa
      781 acgccatcag cgccgcaccc ataccgcccg cagctcctgc gccggggaca tcttttacat
      841 caacatgcag cgctttttta atgacatcgg catagtgaga gaggttattg tccagctcaa
      901 caatcatcgc ttcactggct cccttttgtg ggccaaagat gcgcgatgcg ccgttatcgc
      961 ccaccagcgg attggtgaca tcacaagcga cgcgaatgac gcaatctttt aagcgcggat
     1021 cgaggccgga aatatcaata tcattcagag tattaagact accgccgcca aaaccaattt
     1081 cattgccgtt ggcgtcgcat aatttcgccc ccagcgcctg taccatgcct gcgccgccat
     1141 catttgtagc gctaccgcca atgccgataa taatgtttgt cgcgccgctc tccagcgcct
     1201 gcaggattaa ctcgcctgtg ccgcgtgaag tggtcacgag tggatcgcgt ttttccgcag
     1261 gtaccagctc cagcccactg gccgccgcca tttcaataaa cgcggttttg ccatcgccgg
     1321 agatccccca actggcattc actttctcgc ccagcggccc tgtaacccag gcgtgacgtt
     1381 cagaaccctg ggtggctgca atcatcgctt ccaccgttcc ttcgccaccg tcggcaaccg
     1441 gaatagaaac gtactgtgca tcaggaaaaa tttcccgaaa tcctttttct atcgcctgcg
     1501 caacctcgct ggcagataaa ctttctttat aagagtctgg ggcgattacg attttcatac
     1561 ctatgcctgt taccacatga cgccggaggg cgtttctctt attcggcctg gattccaggc
     1621 ccggattgca atacgccatc cgggcacgac gtcattaacg agtaacttcg actttcgcca
     1681 gtttttcgta gtagcacacc agggcgctat gatccgccgt tcctaaacca tctgctcgca
     1741 gtgcctgcat catctccata accgcagctg tgagcggcag ttgcgcgccg acgccgtggg
     1801 aagtatccag cgcatttgcc agatccttaa tatgcagatc aatacggaag cccggcttga
     1861 agttgcggtc catcaccatc ggcgctttgg catccagcac ggtactgccc gccagtccac
     1921 cgcgaattgc ctgatacacc aggtccgggt taacgcccgc tttagttgcc agcgttaacg
     1981 cttctgacat cgcggcaata ttcagcgcca caatgacctg attcgccagt ttggtgacgt
     2041 tacctgcacc aattgccccg gtatgcacca cggaacccgc cattgctttc atcaaatcat
     2101 agtatttgtc gaaaatagcc ttgtcgccgc ccaccatcac tgacagcgta ccgtcgatgg
     2161 ctttcggttc accgccgctc accggagcat ccagcatatc aatgcctttc gctttcagcg
     2221 cttcgctgat ttcacggctt gccagcggtg cgatagaact catatcgatc aataccgtac
     2281 ctggcttcgc gccttcaata atgccattct cacccagcgc cacctctttc acatgagggg
     2341 agtttggcag catggttatg atgacgtcgc actgttcagc gatcgcttta gccgtagagg
     2401 ctgtttctgc acctgcagca atcacgtcag caatagcttc tgggttacgg tcagcaacca
     2461 ccagcgagta acctgctttc agaaggtttt tactcattgg tttacccata atccccaggc
     2521 caataaaacc aactttcata gtcatatcaa tcatctctct tgttgcggtg gtggttattt
     2581 tttaaaggta tcagccagtt tatgagtggc agagcggaag acgccgagat cgctgccgac
     2641 agccacaaac gtcgcgcccc attccagata acgacgcgca tcggcttcga ccggcgcgag
     2701 gataccgctg ggtttgccgt gcgcgctggc acggttaaaa atgtgctgaa ttgctttttg
     2761 tacatccggg tgtgatgcat tgccgagatg gcctaatgcc gcggccagat cgctggggcc
     2821 gacgaagatg ccgtctacgc cttcggtagc ggcaatggca tcgacgttat ctacgccctg
     2881 ctgactttct atctggacca gaatagtgat gttcttgttc gactgagcga aataatccgc
     2941 cacggtgcca aacatattgg cgcggtgaga aacggagacg ccgcgaatgc cttccggtgg
     3001 gtaacgggtt gatgccaccg cctgctctgc ttcctctttt gtttctacaa aaggaatcag
     3061 gaagttatag aaaccgatat ccagaagacg cttaataatt tccggctcgt tggtcggcac
     3121 tcacactact ggcgcgctgg cgctgccttt caaggccatt aactgcggaa taaacgtgga
     3181 gatatcgttt ggcgcatgtt cgccatccag caccagccag tcaaacccag ccaaaccaag
     3241 aacttcagtg ctaatcgggt tagagagtgc tgaccagcaa ccaatttgta cctgtttcgc
     3301 agccagtgcg gctttgaatt tattcgggaa aacatcgtta ttcatcgctt atacctttgc
     3361 ttatttctgc aattccatac gtttaatgtc gccaactacg aagaggtagc agaccatcgc
     3421 catcagcgct gaacatccca cgaaaaccag tgctgcattg aaggagtgca gttcacttac
     3481 caggtagcca atcaccagtg gagtgacaat ggaggcaaca ttgccaaaga cgttaaagac
     3541 gccgccacag aggccaacaa tctctttcgg cgcggtgtca gaaatcaccg gccagcccag
     3601 agcaccaaat cctttgccaa aaaaagccag cgccatcagc atgaccacca gcgtggtgtt
     3661 gttggtgtag ttacataaga tgatggtgga agccagcaac attcccagca caatcggtag
     3721 cttacgtgcc agggtcaggg ataaaccgcg tttgatcaga taatccgaga agacacctcc
     3781 cagcacgccg cccgcaaaac cacacagtgc cggaatcgag gcgaccagac ccactttcag
     3841 aatcgacatg cctttttcct gcaccagata aatcgggaac caggtgagga agaaccaggt
     3901 gatggtgttg ataaaatatt gtccgaaaaa tacgcccaac atcatgcggt tagagagcaa
     3961 ttgcttgatg taatgcagtt tgggaccgct tgctgccgcg ctgcccggct ttttgtggtc
     4021 catatcgacc accgcgccat tttcagagat aaacttcagc tcttcctcag acatacgtgg
     4081 gtgatctgtc gggttatgaa tcaacttgat ccacagcgcc gtcagcacaa aacaaatcac
     4141 ccccataacg gtaaagacgt gctcccatcc ccaggcgaaa gtcagccagc caagcagagg
     4201 cgaaaagagc gccagcgaga aatattgtgc cgagttaaag atggcggagg cagtaccacg
     4261 ttctttcgtc gggaaccagg cggcgacaat acgggcgttc gccgggaagg atggagcttc
     4321 cgagaatccc agcataaagc gcataaagaa catggagatc cctgcccaag ccagcgggaa
     4381 catatcaaca aagccttgca ggaaggtgaa cagcgaccag aaaaagaggc tgtaggtgta
     4441 aacttttttc gagccaaact tatcaagcaa ccagccgccg gggatttgca tcagcaagta
     4501 ggcccagcca aaagcggaga agatgtaacc catcgaaacc gcacttaact gcaactcttt
     4561 tgccacttcg gtaccagcaa tagacagcgt tgcacgatcg gcgtagttaa cggcggtaac
     4621 aataaaaata atcagtaata ttaaatagcg ggtatgcaca cctttccttt tttcgtcaac
     4681 ggtgtccaga atcattttat ttacctcggg tacttatgct gatttttatt attatgggga
     4741 aggtgttatt tataagtttc atttatgccg taacgacaat gaactcggga attagtataa
     4801 gcagcgcgag aataataatc attgtgcaaa tgctaattta attaatactt tttaaatatt
     4861 attttgagca tatgcacata aggttgcgcg ctaaagcaca gatttgcgct ttaccttgcc
     4921 gggcggcact gcaatcccta aaatgattga cattgatcac atttctgcgt ttaaactcct
     4981 gacattctta tttcacccaa tgaaatcatt tatttttaaa tgagaccagg tcctcatttt
     5041 aataacccct ggctggagaa tattgcacaa tggccaacat cgaaatcaga caagaaacgc
     5101 caactgcgtt ttatataaaa gttcacgaca cagataatgt ggcaattatt gttaatgata
     5161 atggcctgaa agcaggaacg cgttttccgg atgggctgga attaattgaa catattcccc
     5221 aggggcataa agtcgcattg ctggacattc cggctaatgg tgaaattatt cgttatggcg
     5281 aagtgattgg ttacgccgtg cgtgcaatcc cacgcggaag ctggatcgac gaatcaatgg
     5341 ttgtactacc ggaagcgccg ccgttacaca cgctgccact ggcaaccaaa gtcccggaac
     5401 ccttaccgcc gctggaagga tacacctttg agggctatcg caatgccgat ggcagcgtgg
     5461 gcaccaaaaa cctgctcggc atcaccacca gcgtccactg tgtggcaggc gtggtggact
     5521 atgtagtaaa aatcattgaa cgcgatctgc taccgaaata cccgaacgtc gatggcgtgg
     5581 tggggctgaa tcatttgtac ggttgtggcg tggcgattaa cgcaccggca gcagttgtac
     5641 ctattcgtac cattcacaat atttcgctga accctaactt tggcggcgaa gtaatggtga
     5701 ttggcctggg ttgtgaaaag ttgcagcctg agcgcctgct gactggaacg gatgatgtgc
     5761 aagctattcc agtagaaagc gccagcattg tcagtttgca ggatgaaaag catgtcggtt
     5821 ttcagtccat ggtcgaggat attttgcagg tcgccgaacg ccatctacaa aaactgaatc
     5881 aacgtcagcg agaaacctgt ccggcttcag aactggttgt cggcatgcat tgcggtggca
     5941 gcgatgcgtt ttctggcgta acggcaaacc cggcggttgg ctatgcgtct gatctactgg
     6001 tgcgctgcgg cgcaacggtg atgttttcag aagtcacgga agtgcgtgac gcgatccatc
     6061 tgctgacacc acgcgcagtg aacgaagagg tcggcaaacg gctgctggag gagatggagt
     6121 ggtacgataa ctatctcaat atgggaaaaa ccgaccgcag cgccaaccct tcgccgggca
     6181 acaagaaagg cggtctagca aacgtggtag agaaagcgct cggctccatt gctaaatcgg
     6241 gcaaaagtgc aattgttgaa gtgctgtcgc ccggtcaacg cccgactaaa cgcggattaa
     6301 tttacgccgc gacgccagcc agcgattttg tctgtggcac gcaacaggtg gcttcgggta
     6361 tcaccgtgca agtgtttacg accggtcgtg gtacgccgta cggcctgatg gcggtacccg
     6421 tcattaaaat ggcaccccgc accgagctgg cgaaccgctg gtttgattta atggatatta
     6481 atgcgggcac catcgctacc ggcgaagaaa ctattgaaga ggagggctgg aagttgttcc
     6541 actttattct cgacgtcgcc agcgggaaga agaaaacctt ctcggatcaa tggggactgc
     6601 ataaccagct ggcggtgttt aacccagcac cggtgacctg ataattcaat tcgtattatg
     6661 cccgatagca cacgtttatc gggcatattg agtcacagcg aagacgcgtg atccccgacc
     6721 agaatcactt caaccccagc ctttcgcagt ccttccagac tatccgcagg aatgccttca
     6781 tcaacaatga tcatgtcgat acgttgagta tcaatgatct tatgtaaact ggaacgattg
     6841 aatttactgg aatcggtgac cacgatgatc cgttccgcaa cttcgcacat ccgacggctt
     6901 aaacgggctt catcttcatt atgtgtgctg acgccgcgct ccagatcgat cgcatctaca
     6961 ccaagaaaca gcatatcgaa gtggtaattt tgcagcgatt gctcagcctg atcgccgtaa
     7021 aaagattgcg actgacggcg caaatgcccg ccggtcatca gcagctcaac gccttccgct
     7081 tccagcaacg cattagccac gttcataccg ttggtcatcg caattacgtc agtgtacttg
     7141 cgcatcagac gagcaatctc aaaagtggtg gtcccggaat cgaggatcac ccgatgacct
     7201 ggctgaatca actcaacggc agctttcgca acgctgcgtt tcatcgcggt gttcagtgcg
     7261 cttttatctt ccactgatgg ctcgactgac ggcgtcgtgc tatcgcagat caacgcgcca
     7321 ccataggcac gcacagcgat cccctgcttt tccagaaacg ccagatcatt gcggatcgtc
     7381 acagtagata cgccatacaa tgccgacaga tcgttaacct gcacactccc ttgctgtcgc
     7441 agacgctgaa tgatctgttc tcgtcgctcg ctggtgcctg tcactcgctt ctcacctgaa
     7501 gcgtcggtat tactcatagt aagtcctttc gtaaaacttt cgtttcattt cgttttgcct
     7561 attaacgcct ttctattaag caaatgcaag cccaccttgc ccattggcgc aagctactct
     7621 cgtttcagtg actttcatta cgtttctttt gtgaatcaga tcagaaaact attatctttc
     7681 gttttatttt tatctcacca tgacgcagta tcaattgaaa caaaacgaaa gatcaatatc
     7741 gcagtaatct gaactggaga ggaaagtgaa acatctgaca gaaatggtga gacagcacaa
     7801 agcgggcaaa acaaatgcaa tttatgccgt ttgttccgca catccgctgg tgctggaggc
     7861 tgcaatccgc tacgccagtg caaaccaaac gccgttactg attgaagcaa cctccaatca
     7921 ggtagaccag ttcggcggtt ataccggaat gacgcccgcc gattttcgcg gctttgtttg
     7981 tcagctcgcc gactcgttga atttcccgca ggatgcgctg attctgggtg gtgaccatct
     8041 ggggccaaac cgctggcaaa acctgccagc cgctcaggca atggccaatg ccgatgattt
     8101 gattaaaagc tacgttgcgg caggattcaa aaaaatccac cttgattgca gcatgtcctg
     8161 tcaggacgat ccaattccct taactgatga catcgtggct gaacgcgccg cccgtctggc
     8221 gaaagtggcg gaagaaacct gtcgcgaaca ctttggcgaa gccgatctgg agtatgtcat
     8281 tggtaccgaa gtgccggtac ctggcggcgc gcatgaaacc ttaagcgagc tggcggtcac
     8341 cacgccggat gccgcccgcg ccacgctgga agcccatcgt cacgcctttg aaaagcaagg
     8401 tttgaatgcc atctggccac gcattattgc cctggtggtt caacccggcg tcgaattcga
     8461 tcacaccaac gttattgatt atcagcccgc caaagcgagc gccttaagcc agatggtcga
     8521 aaactacgaa acgctgattt tcgaagcgca ctctaccgat tatcaaacgc cgcaatctct
     8581 gcgccagctg gtgattgacc actttgccat tctgaaagtt ggcccggcgc tgaccttcgc
     8641 cctgcgtgaa gccctttttt ctctggcggc gattgaagaa gaactggtac cagcgaaagc
     8701 ctgttccggt ctgcgtcagg tgctggaaaa cgtgatgctc gaccgcccgg aatactggca
     8761 aagccactac cacggtgacg gcaacgcgcg tcgtctggcg cgtggttaca gctactcaga
     8821 tcgcgtgcgc tattactggc cggacagcca gattgatgac gctttcgctc atctggttcg
     8881 taatctggcg gattcaccaa ttccgctacc gctcatcagc cagtatctgc cgctgcagta
     8941 cgtgaaagtt cgctccggcg agctgcaacc gacgccacgg gaactcatta taaaccatat
     9001 tcaggacatc ctggcgcagt accacacagc ctgtgaaggc caataagcaa aacaaagagg
     9061 aacatgctat gccaaatatt gttttaagcc ggattgatga acgcttgatt cacggtcagg
     9121 tcggcgttca gtgggtcgga tttgcggggg caaatctggt gctggtagcc aacgatgagg
     9181 ttgccgaaga tccggtacaa caaaacctga tggaaatggt actggcagaa gggatcgccg
     9241 tacgtttctg gacgctgcaa aaagttatcg acaacattca tcgcgccgcc gatcgacaga
     9301 aaatcctgct ggtttgtaaa acacccgccg atttcctgac gctggtgaaa ggtggcgttc
     9361 cggtgaatcg cattaacgtt ggcaatatgc actacgccaa tggcaaacaa caaatcgcca
     9421 aaacggtttc tgtggatgcg ggcgatatcg cagcatttaa cgacctgaaa gccgctgggg
     9481 tggaatgctt cgttcagggc gtcccgacag agcctgctgt ggacctcttt aaattacttt
     9541 gagggattca tcatggaaat cagcctgttg caggcatttg cgttgggcat tatcgccttt
     9601 atcgctggcc tagatatgtt taacggctta acccatatgc accgcccggt ggtcctcggc
     9661 ccgttggtcg ggctggtact tggcgatctg cataccggaa ttttaaccgg cgatacgctg
     9721 gaactagtgt ggatggggct ggccccgctg gcgggcgcac agccgcctaa cgtgattatc
     9781 ggtactatcg tcggcacggc gtttgccatt actactggcg tgaaacccga tgtcgcggtc
     9841 ggtgtcgccg tacctttcgc tgtcgcagta cagatgggga ttaccttcct gttctcggtg
     9901 atgtctggcg tgatgtctcg ctgcgaccgg atggcggaga acgccgatac tcgcggcatt
     9961 gaacgcgtga actatctggc actgctggca ctcggcactt tctattttct ctgcgctttc
    10021 ctgcctatct acttcggcgc ggaacatgca aaaaccatca ttgatgtcct gccgcaacga
    10081 ttaatcgacg gcctcggcgt cgcgggcggc atcatgccag caatcggttt tgccgtgctg
    10141 ctgaaaatca tgatgaaaaa cgtctacata ccctacttca tcctgggctt tgttgccgca
    10201 acctggctca agttaccggt gctggctatt gctgccgccg cgctggcgat ggcgctgatc
    10261 gatctgctgc gtaaatctcc tgaaccgact caacctgcgg cacagaaaga ggaatttgaa
    10321 gatggcatct aatcaaacca ccctgccgaa cgtcagcgaa aatgaagaaa cactgctgac
    10381 tggcgtcaat gaaaacgtgt atgaagatcg gagcattgcc gcggagctga cgaaaaaaga
    10441 tatcaaccgc gtcgcctggc gttccatgct gttacaagcg tcttttaact acgaacgtat
    10501 gcaggcttct ggctggttgt acggtctgct gcctgctctg aaaaagatcc acactaataa
    10561 acgcgacctg gcgcgcgcca tgaaggggca tatgggtttc ttcaataccc atccgtttct
    10621 ggtgacgttt gttatcggca ttatccttgc gatggagcgt tctaagcagg acgttaacag
    10681 tattcagagc accaaaattg ccgtcggtgc gccgcttggc gggattggcg atgcgatgtt
    10741 ctggctaacg ctactgccga tttgtggcgg gataggcgca agcctcgcgc tacaaggctc
    10801 tattcttggc gcagtcgtct ttattgtgct gttcaacgtg gtacacttgg ggctgcgttt
    10861 tggtctggcg cattatgctt accgcatggg cgtggcggcg attcccctta ttaaagcgaa
    10921 taccaaaaaa gtcggccatg ctgcgtcaat cgttggaatg acggtaatcg gcgcactggt
    10981 ggcaacctat gttcgtttaa gcaccacgct ggaaatcacg gcgggcgacg cagtagttaa
    11041 gttacaggct gatgttatcg acaaactgat gcctgccttc ttaccgctgg tctacaccct
    11101 gaccatgttc tggctggtac gccgcggcta gagtccgctg cgcctgattg cggttaccgt
    11161 ggttctaggg atcgtcggta aattctgcca tttcctttaa atacaatgag gtattagatg
    11221 ttaagtatta ttttgacagg gcatggcggg tttgccagtg gcatggaaaa agcaatgaag
    11281 caaatcctcg gcgatcaatc gcagtttatt gccatcgatt ttcccgaaac ctcaagcgcc
    11341 gcgctgctta catcgcagct tgaagaggcc atcgcccaac tggattgtga agacggcatt
    11401 gtttttctga cggatttgct gggcggcacg ccgtttcgcg tagcttcaac gctggcgatg
    11461 caaaaaccgg gctgtgaagt gattaccggc accaatttgc aactgctact ggagatggtg
    11521 ctggagcgcg aagggttaag cggtgaagaa ttccgtgtac aggcgctgga gtgtggacac
    11581 cgagggctga ccagtctggt ggacgagtta ggccgctgcc atgaagaatg tccggtcgag
    11641 gaaggaatat gacacacgtt ctgcgcgcca gaaggctgct gactgaagag ggatggttcg
    11701 atgaccatca gttgcgtgtt gctgacggtg tcatcgcagc aatcgaaccg attccagtgg
    11761 gcgtgactga acgcgatgcg gaacggctct gccccgctta catcgacacc catgtacacg
    11821 gtggtgcggg cgttgatgta atggatgacg cgccggatgt actcgacaag ctggcaatgc
    11881 acaaggcacg cgaaggtgtc ggcagttggt taccgaccac cgtaaccgcg ccgcttaata
    11941 ccattcatgc ggcgctgaaa cgtattgctc aacgttgcca acgcggcgga cctggtgcgc
    12001 aagtgctggg gagttatctc gaaggaccgt acttcacgcc gcagaataaa ggcgcgcatc
    12061 cg
//
LOCUS       DAJTSI010000123        11924 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000123 DAJTSI010000000
VERSION     DAJTSI010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11924
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.123"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..2341)
                     /locus_tag="OO327_003053"
     CDS             complement(<1..2341)
                     /locus_tag="OO327_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR6019504.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTEGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTLYEARVPVSNSFAPNITFSVLY
                     TRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGELVNVE"
     gene            complement(2275..2898)
                     /locus_tag="OO327_003054"
     CDS             complement(2275..2898)
                     /locus_tag="OO327_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCR6019505.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2895..4583)
                     /locus_tag="OO327_003055"
                     /pseudo
     CDS             complement(2895..4583)
                     /locus_tag="OO327_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4732..7359)
                     /gene="gyrA"
                     /locus_tag="OO327_003056"
     CDS             complement(4732..7359)
                     /gene="gyrA"
                     /locus_tag="OO327_003056"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR6019506.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7506..8228
                     /locus_tag="OO327_003057"
     CDS             7506..8228
                     /locus_tag="OO327_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR6019507.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8356..10290)
                     /gene="yfaL"
                     /locus_tag="OO327_003058"
     CDS             complement(8356..10290)
                     /gene="yfaL"
                     /locus_tag="OO327_003058"
                     /inference="COORDINATES: protein motif:HMM:NF007285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HCR6019508.1"
                     /translation="MMEITANVDTRSTTTEHGRDIEMRADGEVAVDAGVDTQWGALMA
                     DSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDVADIFPYASSLWVGD
                     GATFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNASLFNGDGTLVNATD
                     GVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAGDTLTVNGDYTGGGT
                     LLFDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQFQ
                     NNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPEPDPTPDPDPTPDPDP
                     TPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDGQTLNLRVIGGRYHY
                     TAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGGYSDNQGDSRSNMTGTRADN
                     QNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDVSEHEDGVDRYHSSGIIASLE
                     AGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDDIQTRLGLHSEWRTA
                     VGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKLRGEIKVGVTGNISQRVSLRGSVAW
                     QKGSDDFAQTAGFLSMTVKW"
     gene            complement(10300..11895)
                     /locus_tag="OO327_003059"
     CDS             complement(10300..11895)
                     /locus_tag="OO327_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AidA family protein"
                     /protein_id="HCR6019509.1"
                     /translation="MTNNASGGAVFLQQGAEFSLLPENETGMTLFANNTVTGEYNNGG
                     AIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNAINNIANDGKGG
                     AIYTINNDVYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTIINNTAF
                     TNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPSTAAGG
                     FMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFTG
                     EMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQNQAELNVGSTQQTF
                     VHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSYVLAGAQSMALTGD
                     IVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSGS
                     SGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASGT
                     LMVSDNSQLGDTHYNRQVIFTDK"
BASE COUNT         2757 a         3307 c         2945 g         2915 t
ORIGIN      
        1 attcgacatt gaccagttca ccaggctggt aatgggtttt gtccgttttc acccggatat
       61 caagctgagg aacggcaact ttgatcccgg cgttctgaaa actgtactga ccattacggg
      121 tatacagcac cgaaaaagtg atgttaggcg caaaggaatt gctcactggt acccgggctt
      181 catacagggt atcgtttaaa cgttgtagcg ttagccagtt tgccggatgc gaaagcagcg
      241 actgctgttc cacgcgatcg cgttccagcg tcaataacgc ttcatcaatt ggctccggga
      301 aagtaatcag catcttcgcg gtttcgcctg gctggtacag cgttttatcc gccacgatat
      361 ctaccgtacc agtatgcgcc gtgctgccct taccgctgac ggcatgactt aacccagcga
      421 gaattaagcc gtctttatcg cgtaatgtca gattgtagtt gccaggttta gcgaaattga
      481 cggtaaagga tttgccgcct gacggtagct ctccgctatg gctcgtgcgg tcttcgagac
      541 gcaaccattc atacgtaaca ggaacctgtt ttgaagattc cagcgcggca taacggaaca
      601 caaccgactc gccgctatta ctgtattgtg cggcggtact taaagagtaa tgcgccaggc
      661 cgcgttcaat gaggatctct ttggtggtgg tgacgcgata cgccgcgccg tcactggcgg
      721 agacggttaa caaatagcgg ctcggtttat cggcggcggg gagatttaac gccacatgac
      781 cgctggcgtc ggacaccgtt tcgctgcctt ccagcgacac ggggaaacgt ccggcataac
      841 gcaaatcgtt accgaccatt gataattgct gagcgcgcaa acttaactgc acgcgggcat
      901 tttttaccgg ctcgccatcc gggtagagca gttgcagttt gccgctgacc gcttcgccag
      961 ttttgaactc ttttttgtcg agagctaaac caatctcgaa atgtggcttg atgtagtttg
     1021 ccacgcgaaa actgctgcta tagacctgat tgcggtaagc aagacgtaac tcataacctc
     1081 cggcaacggc attttctggc aggcggaaac ttccctgccc gccattgcgc gcatccagcg
     1141 tgacatcgac ggtttgcaac agactgccgt tagcgtccag caccgaaagc ttcgccgggg
     1201 cgctgacgat gggggatgaa tgcaacggat cgcggaactc gcggcccatc actttaacat
     1261 cgacacgatc gcctgcgcga tatagcgggc gatcggtaaa aatatacaag cgggtgttgt
     1321 agatttcgct ttcgtagaag aagttctcgg agacaaaaac gccgccttca gcatccttac
     1381 ccagaatgta tgaacgttct ggcgatatat gttgtaactg caaagtaccg ctgtcatcgg
     1441 tcaccccgcg ggtcatcacg ccaagacctt cagtccacaa gatctctgag ccgggcttcg
     1501 cttcaccctg ttttttaccc gcggtccata ccagaagctc gttacctgac actttgctaa
     1561 gcgccacggt atcggaaaca aacaccaccg tcgtcgcccg atacccacct accatcgcct
     1621 cgacgaggta cagccccggc tcttgttggc cgagaggaat ataaatatta cccggctgcg
     1681 gcgagatgaa attgctggat gcgccttcca gttttacccc ttgctgcggc tcgaccggtt
     1741 tagcctgcca tagtggataa cggaactgtt ccaccaaggg atattttttc agcggggaaa
     1801 actggttgtt ctgtacataa cgggaaggtt taataatggc attgccgaga tgtaattcgg
     1861 gtaatgcctg agtcacattc tgccgtgact gagaagagaa agtacgctgc atcacgcggc
     1921 gagatttgcc gtaccagtta tcccacagcc aggtcagcgt attgttcagc ccgtcgccca
     1981 gatattgcgg ttgcaccaca atgcgatgca ggtttttctg ctggcgcaaa aatgccatcg
     2041 ggtcaggaat acgatatagg cgaacgtcca cgccgccgta ctcttccatc tgatagcgcc
     2101 gataatcacg ccccggcgct tccagtcgca ctttcgcctc ttcactgctg ctaaaactgc
     2161 tgtcagcaag caaaaagaat gttcccccgg cgggcggcgc atagttactg gaaggaagcg
     2221 aatcatcagc tttagcaagc cctgttccca caagagacag ggacaagcac gctgctatcg
     2281 cgccagaaaa tttaaacgat aaatgccaat gaaattggga ttggaatcgt tgggtatcca
     2341 tcgggtgtcc ttccatgtca taagttgttg cagactgact gcgcgcattc cgttgtcagt
     2401 tttcgtggcg cttccggtgt ggtagatgac gtaacgcccc atccagacca ttaagtgctg
     2461 ggcatcgccc tgatcgaaaa aaatcatatc gccaggcagc gcctggttta tgtcttggcc
     2521 aataaactgg ctgttgtact gaatcaaatt aatcgcggtc acgtaggggc cgtttttccc
     2581 gctcccctga ttccagtttt gcgccagttg acgctgttca ggtgttagcg tcatctctgg
     2641 cggcaaatac tggctagata aaccgttact ttttagccat ttactgtcgt gaactttcag
     2701 cgtctcgttc gccgcaaatc gcaccaggcc cgcacaatcc tgctgatacc agcgtggact
     2761 tggcccctgg cggagctgtt cttgtgcaat gcgtacaaac caggcgcgaa acagcctgga
     2821 ttgttcgaca ttcagcattt cgctatgggc aacaacacaa cacagccagc aaatcagcgc
     2881 cagcagcccg tgtctcatag cggctgccag gttatgggta gccactgcca ggcgacaccg
     2941 ggttccatct gggccagctt catgacataa cgcggttgtt gagataaagc gtccagcttc
     3001 ggcattaata aagtttgtgc ggcgttataa aaaaccggtt cgagattctt cggcagactg
     3061 gtcaacgttt cgttacgcag cagtttcgcc acgccttgtg gattgatata gagcggaacg
     3121 atgccatcag tgggtattac atccaccatt gccgggcggg ttttattcag tgtttgcagg
     3181 gcgttattaa ccagcgtgtc atcgagggag aaaagcagcg ttttgttttg catcgccagc
     3241 gacacacgga aaaaataatc cgacattaat tgatcgggtt gcgccgcctg cgctttcgga
     3301 tactgaccgt atcgggaact cacttcgcga caccagattt gcgcttcgcc ctgctgagtc
     3361 tggcttaccg gcaaaacacc ttctggcgct ttgctttcgt gcgcaccaat attttgcgta
     3421 aacagtttcc ctggcaattg cgcctgttcg gcagtgccat caaactgacc gacaaacagc
     3481 ggggtttgca attttgagtc ttcataccag cacagccccg cggcaccgtc taacgcccca
     3541 ttcagcttgt cgttttcctg gctgatgtgc gaaagcatct cttcggcaat accgtgtgaa
     3601 tacggcaccg ccacacagaa gctggctccg gcaggcatac tgttccagac cggcgtaaaa
     3661 tcgaaactgg catctacgct ggcggattca tcatttaacg ccacaaaact gtgccagccg
     3721 tcgttaccca tttcgaagcg tacgccagca aaagaaggca ttaaccgctg gtagccaaaa
     3781 cccagcaacc tggcgctgac cacgatgcgc aggcgtactg gcgttttttc ggctgtacgc
     3841 tcttccaggc caaagctggc ttgccagcgt tttttaccgc tcaacaaatc acttgcgatc
     3901 gccgtggctt cggtatcctg ctgatcatct ttaaacaaca tatccgtact ggaaaacacc
     3961 agcatcttgt cctgatatgt cgcgaacatc agggcgttat tgccgttata gcgcaactga
     4021 taaacaggaa tagtttcact atttatcttg atgctactga tttccgtttt gcttaactag
     4081 ctgtcgctgg tagcggcaaa caacaatggc tccagcagtt tgcttaaacc actgcgctgg
     4141 atcagcacca tgtaatgtga aagatggcct tgtttatcgt gccatagcgc cgcctgcgcg
     4201 ggctgatcta agagtgacga aaagagctta tctttcagcg tgagatcgtg ttcatagaca
     4261 atgcggcgaa tgctgccttc aatgcccaga cgatcggcat gattctgata atagaaaacg
     4321 aaatcttcgc tcagaacatc gtggagaaac ggaatggtga ggagatcttt gggaagctgg
     4381 ctcagagagt cgctgtctag aaagaggtcc ggctcattga gatcgatttg cagattgttg
     4441 tgcaccacca gcggcgacaa cgttttttct ggcccactgc cagcatattg caacgcccag
     4501 acgccagcgg aaagcagtgc tattgcgcca aaacctacaa gaccatagaa ccgccagcct
     4561 ttcgcctttt tttcaccact cattgccaca ttccttgtgt atagccagcc attttttacg
     4621 ggcacagcca aactttaccg cgccctaata cgacaaaagc ccagactttg cagcctggac
     4681 ttttcaattc aaacaaggga gatagctccc ttttggcatg aagaagtaaa attattcttc
     4741 ttctggctcg tcgtcaacgt ccacttccgg agcaatttca tcatcccctt ccgcggcact
     4801 gccgtcgatg gtatccagat cttcctcgtc aaccggttca gcaacacgct gcagacccac
     4861 tacgttttca tcttccgcag tacggatgag gatcacgccc tgggtgttac ggcccacgat
     4921 gctgatttct gaaacgcgag tacgtaccag cgtaccggca tcggtgatca tcatgatctg
     4981 gtcgcagtca tctacctgca ccgcgccaac cactaaaccg ttacgttcgg taaccttgat
     5041 ggagataacc cctttcgtcg cacgcgactt ggttgggtat tccgccactg cagtacgttt
     5101 accgtaaccg ttttgcgttg cggtgaggat tgcgccatcg ccacgaggca cgatcagaga
     5161 gacgacttta tcgccttcac ctaagcgaat accgcgcaca ccagtggtat tgcagcccat
     5221 cgcacggaca gaagactctt taaagcgcac cactttacct tcggcggaga acagcattac
     5281 ttcatcttcg ccactggtca gatcaacgcc gatcagctca tcgccgtcaa ccagtttgat
     5341 cgccacttta ccggcggtac gcagacggtt gaattcggtg aggacggttt tcttcacagt
     5401 accgttagcg gtcgccatga agactttcac gccttcttca aactcggtca ccggcaggat
     5461 cgcggtgata cgttcgtcct gttccagcgg tagcaggttg acgatcggac gaccgcgcgc
     5521 gccacgagtg gcttccggca actggtaaac tttcatcgaa tagacgcgac cacggctgga
     5581 gaagcacaga atatggtcgt gcgtgttcgc caccagcagt cggtcgataa agtcttcttc
     5641 tttaatacgt gcggcagatt tacctttccc gccacgacgc tgcgcttcgt attcagaaag
     5701 cggctgatat ttaacgtagc cctggtgaga gagcgtcacg accacatctt cctgggtgat
     5761 cagatcttcc aggttgatgt ctgcgctgtt ggcggtgatt tcagtacgac gtttgtcacc
     5821 gaactgttca cgaaccagct ccagctcttc gcggatcact tccatcagac gatcggcgct
     5881 accaagaata cgcaacagtt ccgcgatctg atccagcagc tctttgtatt cgtcgagcag
     5941 tttttcgtgc tcaaggccgg tcagtttctg caaacgcaga tccagaatcg cctgagcttg
     6001 ctgttcggtc aggtagtaca gaccatcacg cacgccgaac tctggctcca gccattccgg
     6061 acgcgcagca tcgtcgccag cacgttccaa catggcggca acgttgccca gctgccacgg
     6121 attagcaacc agcgcagttt tcgcttctgc aggcgtcggc gcatgacgga tcagttcgat
     6181 gatcgggtca atgttcgcca gcgccacggc taatgcttca aggatatgag cacgatcgcg
     6241 agctttacgc agttcgaaaa tagtacgacg ggtcaccact tcacggcggt gacgaacaaa
     6301 cgccgcgatg atgtctttca ggttcatgat cttcggctga ccatggtgca atgccaccat
     6361 gttgataccg aaagaaacct gcaactgggt ctgggagtag aggttgttga gcacaacttc
     6421 accgaccgca tcgcgtttca cttcaatcac gatgcgcata ccgtctttgt cagactcgtc
     6481 acgcagcgcg ctgatgcctt ccacgcgttt ttcttttacc agttccgcaa tcttctcgat
     6541 caggcgcgct ttgtttacct gatacggaat ttcgtggacg ataatggttt cacgtccggt
     6601 tttggcgtca acttccactt cagcacgggc gcggatatat accttgccgc gaccggtacg
     6661 gtaagcttct tcaataccgc gacgaccgtt aatgattgcc gccgtcggga agtccggccc
     6721 cgggatgtgt tccatcagcc cttcaatgct gatgtcttca tcatcgatat acgccagaca
     6781 accgttgatg acttccgtca ggttgtgcgg cgggatgttg gttgccatac ctacggcgat
     6841 accggaagaa ccgttcacca gcaggttagg aattttggtt ggcatgacgt cgggaatttt
     6901 ttccgtgccg tcatagttat caacgaaatc gaccgtctct ttttcgagat cggccatcag
     6961 ttcatgggca attttcgcca gacggatttc cgtataacgc attgccgccg cagagtcgcc
     7021 gtcgatggaa ccgaagttac cctgaccgtc taccagcatg taacgcagcg agaatggctg
     7081 cgccatacgg acgatcgtgt cataaaccgc cgagtcacca tggggatggt atttaccgat
     7141 tacgtcacca acgacacggg cagatttttt ataggctttg ttccagtcat tgcctagtac
     7201 gttcatggcg taaagtacgc gacggtgtac cggcttcagg ccatctcgga catctggcag
     7261 cgcacggcca acaatgaccg acatcgcata atccagatag gagctcttta gctcttcctc
     7321 aatgttgacc ggtgtaattt ctctcgcaag gtcgctcatc taaccgctat ccctctactg
     7381 tatcccggat tcaaaggtcg caaattataa cacagccgcg cagtttgagg taaacctata
     7441 cgctttattc acatccaatg cctgatatac tcgtttgtct tgccaattac ggagtagaag
     7501 tgccaatgaa tgccgaaaaa tcgccggaaa accataacgt agaccacgaa gagatcgcta
     7561 aatttgaagc cgtcgcctcc cgctggtggg atcttgaagg tgagttcaaa ccgttgcacc
     7621 gcattaaccc gctgcgtctg ggctatattg ccgagcgtgc tggcggttta tttggcaaaa
     7681 aggtgctcga tgtcggttgt ggcggcggca ttctggccga gagtatggcg cgcgaaggcg
     7741 cgacggtgac cggtctggat atgggctttg agccattgca ggtggcaaag ctgcacgcgc
     7801 tggaaagcgg cattcaggtg gattacgtgc aggaaaccgt ggaagagcac gcggcaaaac
     7861 atgccgggca gtatgatgtg gtgacctgca tggagatgct ggagcacgta cccgatccgc
     7921 agtcagtggt cagagcctgt gcgcaactgg tgaaaccagg cggcgatgtc tttttctcga
     7981 cgcttaaccg caacggcaag tcatggctga tggcggtggt tggcgcggaa tatattttgc
     8041 gcatggtgcc caaaggtacg catgatgtga agaagtttat taaaccggca gagctgcttg
     8101 gctgggtgga tcagaccagt ttaaaagagc gacatataac cgggctacat tacaacccga
     8161 tcactaattc ctttaagctc ggccccggcg tggatgtgaa ctatatgctg cacacgcaga
     8221 ataagtgagg ttgatgtttg gccgcgccaa tgcctgatgc gacgcttgca gcgtcttatc
     8281 aggcctacaa atgctctccg taggccagat aaggcgttta cgccgcatcc ggcaattgtg
     8341 atggtcagac agcgattacc atttcaccgt catcgacaaa aaccctgccg tctgggcaaa
     8401 atcatcactc cctttctgcc acgccacgct gccgcgcagg gacactcgct gactgatatt
     8461 gcccgtgact cccactttta tttcaccccg tagcttcacc gcatcgtcac tgatagtgct
     8521 gccatcttct tcaatttccg tcgcatgggg atcgtgatag taattcagat ctaatgttgg
     8581 tgtgacacca accgcggtac gccattcgct gtgtaaaccc agccgcgtct gaatatcatc
     8641 cccctgcgat tgtgacacgc gcgcatggtt agcggcggta aaatcatcct gctgcacgcc
     8701 ctgataaatc acctgcgcct gcggttcaat caccacacca cgccccggta accactgata
     8761 cccagcttcc agcgaggcga taatccccga cgaatggtaa cgatccacgc catcttcatg
     8821 ttcggaaaca tcattgttaa accacgcgta ttgcagccag ctatccagcc aggccccctg
     8881 cttctgatta ccgtgctgat accagcttga ggtcagccca acggcataac cgtggttctg
     8941 gttatcggcg cgagttccgg tcatattcga gcggctgtcg ccctgattat cactgtagcc
     9001 accaaccgcc ccaagcatcc actcgccatc cgtgccccag cgcccgctaa acaagccgcc
     9061 gctaagctgc accgtagaag tgtcttcctg ctgagccagt tgccctgccg ccgtgtaatg
     9121 ataacgtccg ccgataacac gtaaattcag cgtctgacca tcgccaccag cgtgatcgcg
     9181 tcgctccatc ataaacgcct gatttgccgc ccgcaggtta ttgagataac cgccaacttt
     9241 ggcattcagc accggctggt aagcaggcgt aggggccggg tcgggggttg ggtcaggatc
     9301 cggcgtggga tcaggatcgg gagtcgggtc tggctcaggt ggcgatggcg gcgttacttc
     9361 ttgcgatcgc agataccagt cgttgttatc ttccactagc gtgtagtcat acgctcccat
     9421 gttgacgtag ccactgccag ccagactgaa ctgtgcattg ttttgaaact gcgtgggatc
     9481 agctgcgaaa tcaaccactt taatgcctgt cgatgtcggc tcaccaatcc ctgtaatgga
     9541 gttaaccacc acagttgtgt tgccagcagt attaccgttc atcaccaatt gatcgcttac
     9601 cgagtcatcg ccgtttaatt cgctgtcgaa tagtagcgta ccgccgccgg tataatcacc
     9661 gtttaccgtc agcgtatcgc cagcgacgcc attttgcagg ctaaccgcac cggacgtatt
     9721 ggtcagatca ccattaaccg tcacgtcagc aagataggtc aggctgtcag tttcaaggtt
     9781 ggtattaagc tcgcctgtca acgtcacgcc atcggtagca tttaccagcg tcccatcgcc
     9841 gttaaatagt gaggcattaa gggcgacgga agtatcctgc ccggtcagac gcaaaaccgt
     9901 accatcgctg atgtcgatag tgccgctgga agtagcatca attgactgaa tatcctgatc
     9961 cgcgccagta acgaacgttg ccccatcacc aacccacagt gacgaagcat aaggaaagat
    10021 atccgcaaca tcgcctttca gggtgccttc ttcgacacgt accgccgact gcgttgtacc
    10081 gctggcggtc agctccagcg tacccgcccc cgttttagta aatgtgctac cctcatcctg
    10141 atgctgcccg ctgctgtcag ccatcagtgc gccccactgc gtgtctaccc ccgcatcaac
    10201 tgccacttca ccgtcggcgc gcatttcaat atcacgccca tgctcagtcg tcgtagagcg
    10261 agtatcgaca ttggcagtaa tctccatcac gctttcttgt tacttatcgg taaagataac
    10321 ctggcggtta tagtgggtat ctccaagctg cgagttgtcg ctcaccatca gcgtaccgga
    10381 ggcgatttgt gttgtgccca aataactatt attgtttgcc agacttacct gcccgtcgct
    10441 cgcatcgacc accacgtaga cgccatcttt cccgtcgccg ccgatatgca aattgtcgcc
    10501 acctgcaaga tctaccacat cctgactgcc gataaccgtt ccgctgctgc cactgacttc
    10561 aaggccatca ttgtatgatg tgccgctctg ccaagtggag aaatcagaga gatcgagcac
    10621 accgccgttt aacacgatcg actgcggatc gtcctggaga gcggtaagat ctgccgcgtc
    10681 gccttccagc gaaagcaccg caccatcatc aacgactata tcgccggtta gcgccatcga
    10741 ctgcgcccct gccagcacgt agctgccgtt ttgcgcaatg gtgagctgac cagcaccttc
    10801 gatgatgcca gcaaaactgc cctgattaac agtaacgttg ccaccagcat cgatatttaa
    10861 agtgccattc tgaaagcccg tcaatgcgtg cacaaaagtt tgttgggtcg agccaacgtt
    10921 tagctcagcc tgattctgat actgatcaat actccctatc gtcagaccgt agcagtcttg
    10981 cggatcgtcc tggcaatgcg tatcgccgac attcatcagg gagttgctgc gacccagggt
    11041 aacttcaccg ttttcaatct gcatctcacc agtaaagtca ttgttatctg cattaagtac
    11101 cagatcgccg gaacctgttt tggtgattaa cccggtacca gcaatagagt caacagctcc
    11161 gtcattctct gtattgccga taaccagcgt ttttccgtcg gcaatatcaa aggtaacttc
    11221 acttaagccg agatacataa agccacccgc cgcagtggag ggaccatctc catagcctgc
    11281 tgcgctattg ttctcatcga ctaacacgcc gccgttctgg ctgtagctgt catcaacaga
    11341 aatatcaata agatagggag ccgtcgcgct attggtatat atcgccccgc cataaccttc
    11401 ggctgtgtta tttgtaaagg cggtgttatt tattatcgta taacctgaag gatgcttgct
    11461 gtcgctatta ttatcggtaa catcgattgc cccgccatcg ccatcactgt aacttgttga
    11521 tgtatatgcc tggttattgt taaaaacatc atcacttaaa tagacgtcat tattaatggt
    11581 ataaattgcg ccacctttgc catcattagc gatattattg atggcgttag tgacacgtaa
    11641 atcgacggca ccagtatcat tagtaccaga agaatagatt gcgccaccat agccgcctgc
    11701 gacgttaccg gaaaaaataa catcagtaag attcagcgtt gagttttctt tagcaaatat
    11761 ggccccgccg ttattatatt ctcctgtaac ggtgttattg gcaaacagag tcattccagt
    11821 ttcattttct ggtaatagtg aaaattccgc tccttgttgc aaaaatacgg ccccaccgct
    11881 ggcattattg gtcatatccg aaaaaaccag ccattgccca tcgg
//
LOCUS       DAJTSI010000124        11783 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000124 DAJTSI010000000
VERSION     DAJTSI010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11783
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.124"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            38..778
                     /locus_tag="OO327_003060"
     CDS             38..778
                     /locus_tag="OO327_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR6019510.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            837..1223
                     /locus_tag="OO327_003061"
     CDS             837..1223
                     /locus_tag="OO327_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR6019511.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            1232..1561
                     /locus_tag="OO327_003062"
     CDS             1232..1561
                     /locus_tag="OO327_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR6019512.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            1533..4574
                     /locus_tag="OO327_003063"
     CDS             1533..4574
                     /locus_tag="OO327_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6019513.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            4574..4903
                     /locus_tag="OO327_003064"
     CDS             4574..4903
                     /locus_tag="OO327_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6019514.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            4903..5601
                     /locus_tag="OO327_003065"
     CDS             4903..5601
                     /locus_tag="OO327_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR6019515.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            5606..6349
                     /locus_tag="OO327_003066"
     CDS             5606..6349
                     /locus_tag="OO327_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6019516.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            6286..6888
                     /locus_tag="OO327_003067"
     CDS             6286..6888
                     /locus_tag="OO327_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR6019517.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            6949..10428
                     /locus_tag="OO327_003068"
     CDS             6949..10428
                     /locus_tag="OO327_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR6019518.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDVQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            10496..11095
                     /locus_tag="OO327_003069"
     CDS             10496..11095
                     /locus_tag="OO327_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR6019519.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHRSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            11159..>11783
                     /locus_tag="OO327_003070"
     CDS             11159..>11783
                     /locus_tag="OO327_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6019520.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMA"
BASE COUNT         2770 a         2791 c         3790 g         2432 t
ORIGIN      
        1 acgtaccaga tcacgtacac gaattaagga ggtggtaatg accacaccaa atccactggc
       61 aaagacgaaa ggtgcgggga cgacgttctg gatgtatacc ggcaacggcg atgcgtttgc
      121 gaaccctttg tcggacactg actggctgcg tcttgcgatg gtgaaggatc tgcaacctgg
      181 cgaaatgacc gctgatgcag aagatgacac ttatctcgat gatgaagatg cagactggaa
      241 aacgacaacc caggggcaga aatccgttgg tgatacttcg gcgacgctgg cctggcgtcc
      301 gggtgacagc gggcagaaaa aactggttca gttgttcgac tccggtgaag tctgcgcgtt
      361 tcgtatcaaa tatcccaacg gcactgttga tgttttccgc ggctggctga gctcactggg
      421 taaaaccatt gcctcaaaag acgtgatgac ccgcactgtg aaaatcagcg gtgtggggcg
      481 tccgtatctg gcagaggaag gcactgaaac cgtgggtgtt accggactga cggtggcacc
      541 ggcatctgtc agtgtcaaag cgggagcaac caccacactg acctttacag taaaacctga
      601 cggggccagt gacaaagcga tcagtgtgca ttcgtcagat cctcagactg cttcggtgac
      661 cctgagcggg cttgtggcca cggtgaaagg cgtgaagcag ggcagtgtca gcatcgtggg
      721 catgacctct gacggagagt ttgtggcagt ggctgcggtg accgtcagcg caccataaca
      781 ggacgatact catcattgcc ccggttatcc ggggcttttt gcatccggag aacatgatgt
      841 ttctgaaaca ggatacgttt aattatgaaa aacagtccgt ggtgctcagt gagctgtccg
      901 ggctgcagag aattgaatat ctgacgtttg ttcagcagcg aacggcaaag tttgatgcac
      961 aggagggaga actgccggag gctgaacgac agattgcttt tctgcgtatg ggaatggata
     1021 tcaatgcctg gctggtttcc cgctcactgt ggaatgctga gcagtctcag gatgttgaga
     1081 cgctttgcgc atccattatg acaacatggt cgtatgatgc gctgggcgcg ggggcggaga
     1141 gggttctgtc gctgagcggt atggggacca ttgagaatgc cggggatgat gatcatgagg
     1201 cgctgacgcc ggaaaagtcc tgacgcggga aatgcagttt gtcatgcggc ttgcccggga
     1261 gttccggcgg gcagactggc ggcggatgct gtcggatatg tcggccactg agcttggtga
     1321 gtggggcgat tatttccgga tgcagagctt cagtgatgtg tggatggatg cgcagtttgc
     1381 ctcgctgaag gcattgatcg tgagaatggt gtccggcagc agcgatgctg cggtggctga
     1441 tttcagcctt ttaccggaag agaacgggat accggagcga acggacgaag aactgatgca
     1501 tcttggggaa ggtatttccg gaggtgtgcg ttatggacca gatagccaac ctggtcattg
     1561 atttggggat tgatgcggca gagtttaaaa atgaaattcc ccgtatcaaa aaccttctga
     1621 atggtgcagc cagcgatgca gaacggtctt ctgcccgtat gcagcgtttt atggagcgtc
     1681 agactcaggc ggcccggcag acaatgcagg cggcttcttc ggctgcaaca gcagcatcag
     1741 cccatgcgca gacggtggag aagaatgcac gggctcatga acgcatggcc cgtgaggtgg
     1801 aacaaacccg tctgcgcgtg gatgccctga atcagaaaat gcgcgaggaa caggcgcagg
     1861 ccagggcact ggcggaggcg caggataaag cggctgccgc cttttatcgc cagattgaca
     1921 gtgtgaaaca ggccggtgcg gggcttcagg aattacagcg tattcagcag cagatccgac
     1981 aggccagaaa cagtggcggg gttggtcagc aggattatct ggcgctgatt tcggagatca
     2041 cggcgaaaac ccgtgccctg acgcaggcag aggaacaggc cacccggcag aaagcggcgt
     2101 ttatccgcca gcttaaagag caggcaaccc gccagaatct gtcgtcttct gagttgcttc
     2161 gcgccagggc ggctcagctg ggggtaagca gtgctgcaga agtgtatatc cgcaaaatgg
     2221 agcgggcagg aaaagccaca cattcgctgg ggctgaaaag tgcggcagcc cggcgggagc
     2281 tgggggtgtt aatcagtcag atggcgcgag gcaattttgg tgcgctgagg ggatccggga
     2341 taacgctggc taaccgtgcc ggatggacag gcgcactgat gtcgccgaaa ggcatgatga
     2401 ctggcggcgt tattggcgga cttgtcgcgg cggtcctggg tctgggtaaa gcctggcatg
     2461 acggccggaa ggagggcgag aaatttaacc gtcagctggc gctgacggga cattatgccg
     2521 gtgtcactgt cgggcagttg tggaaactca gccgggccat atccgggaat ggtatcacgc
     2581 aacatgcggc agccggtgcg ctggcacagg tggtgggcag tggggcattt catggaaacg
     2641 atattggtat ggtggcgaaa gctgccgcac agatggagcg atcggttggc cagtcggtca
     2701 gcgataccat aaatcagttt aagcggctga aggatgatcc tgtaaatgcc gcgaaggcgc
     2761 tggacaatgc gctgcatttt ttgaatgcca ctcagcttga gcagatacgc gtccttgggg
     2821 agcaggggcg gtccagtgat gcggcccgga tcgccatgtc tgcgctggca gaggaaaccg
     2881 gtaaacgcac atctgatatt gataataatc tcaatgcgct gggtagtacg ctgcaaacct
     2941 tgtctgactg gtggaagcag ttctgggatg cggccatgaa tattggtcgt gaagactcgc
     3001 tggatgcgca gattgatgcg ttacaggaaa aaattcagcg cgcgaaaaaa tatccgtgga
     3061 caaacgcctc cacacaggtg gagtacgatc agcagcgtct taacgatctt caggagaaaa
     3121 aacgccggaa ggatttgcag gatgcaaaag cgcaggcaga acggaattac caggagcaac
     3181 agaaacgccg gaatgctgaa aatgccgcgc tgaaccggat gaatgaaacg gaagctgcac
     3241 gacatcagcg ggaaattgcg cgtattaatg ccatgcagta cgccgaccag gctgtcaggg
     3301 atgcggcgat acagcgtgaa aatgaacgtt acgaaaaagc cattaagaaa aatacacggg
     3361 caacccgtaa tgatgaggcc acccggttat tgctgcagta cagtcagcag caggcacagg
     3421 tggaagggca gattgctgct gccagacagt cagcaggcat tgccactgaa aggatgactg
     3481 aagcgcataa acagcttctg gccctgcagc agcgcatcag cgacctggac gggaaaaagc
     3541 tgacggcaga tgaaaagagt gtgctggccc gtaagaatga gctgattcag gcgctgacgc
     3601 tgctggatgt gaaacagcag gagctgcaga aacagacagc gcttaacgac ctgagaaaaa
     3661 aaacggttca gctgaccagc cagctggcag acaaagaacg tgcactgcgt gagcagcaca
     3721 atctggatat tgccactgca ggtatggggg ataagcagcg gcagcgctac caggcacagt
     3781 tgcgcatccg gcaggaatac cggcaacagt tgcaacagct tgagaatgac agtcgccaga
     3841 aaggcactta cgggacggag gactaccgga gggctgagga ggtgctgaag gggagcctga
     3901 agcgacaact gaatgaaaac aaacgctact ggcaggaact ggaagtggcg cagggcgact
     3961 ggaaaaacgg tgccatgcgg gcgtttcaga attttacggc ggatgcggat aatgcggcgg
     4021 gaacggcaga acagatgttt acagtggcat tcagcagtgc cggtaatgca ctggcgacat
     4081 tctgtaccac cggtaagctg aattttaaat ccttcacctc ttccctgttg tcagatatgg
     4141 ccagaattat ggcacagatg gccatgatgc aggcggtaaa gggcgtcggt tctttattcg
     4201 gcttcacgac taatgctgat ggcggtgttt accagtctgc tgatttgagt cgctacagtg
     4261 gcacggtggt taaccgtccg acgttttttg cttttgcaaa aggcgcgggt gtgatggggg
     4321 aagcgggacc tgaagccatt ctgccactgc gtcgcggtgc tgatggtaag ctgggggttg
     4381 tggcggatac tggtggttca ggcatggtga tgtttgcccc gcagtacaac attgagatca
     4441 ataacgacgg cacgaacggg cagataggtc cggctgccct gaaggtggtt tatgacctcg
     4501 ggaaaaaagc ggccgcggac tttatgcaac agcaggcccg tgatggtggc cggttaagtg
     4561 gagcataccg gtaatggaga cgtttcactg gaaagtgcgc ccggatatga atgtggtatc
     4621 agagccgaaa gtggtgacag tgaagctggg cgatggttat gaacagcgtc gtgcggcggg
     4681 actgaataac cagttgtcga cttacagcgt gacgatacgt gttcgtaaag gtgaacaccc
     4741 atctttaaaa gcctttctgg aacggcacgg tggcgtccgc gcatttcagt ggacgccacc
     4801 ttatgactgg aaactgatca gggtggtttg tcgtaaatgg tcggcaagcg tgggggcgtt
     4861 atgggtgact gtaacggccg attttgaaca ggtggtgaac tgatgcagga tatccggcag
     4921 gaaacactga atgaatgcac ccgtgcggag cagtcagccc gggtggagct ctgggaaatc
     4981 gatctgacag aggtcggtgg tgagcgttat tttttctgta atgagcagaa cgaaaaaggt
     5041 gagccggtca cctggcaggg gcggcagtat caggcatacc ccattcaggg gtcggggttt
     5101 gaactgaacg gcaggggctg tgctgcccgt ccgacactga cggtctctaa cctgcacggc
     5161 atggtcaccg ggatggcgga agatctgcag agtctggtcg gcggaacggt ggtccggcgt
     5221 aaggtttacg cccgttttct ggatgcggtg aacttcgtca acggaaacag cgacgccgat
     5281 ccggaacagg aggtgatcag ccgctggcgc atcgagcagt gcagcgaact gagcgcggtc
     5341 agtgcctctt ttgtgctggc cacaccaacg gagacggatg gcgcggtttt cccggggcgt
     5401 atcatgctgg cgaatacctg tatgtggact taccgttctg atgagtgtgg ttacacgggc
     5461 agggcagtgg ctgacgagtt cgacaaacca acaacggata tccggaagga caaatgcagc
     5521 aagtgtatgc gcgggtgtga gttgcgcaac aataccggta atttcggcgg tttcctttcc
     5581 atcaataaac tttctcagta aatccatgac acagacagaa tcagcgattc tggcgcacgc
     5641 ccggcgatgt gcgccagcgg agtcgtgtgg cttcgtggtg agaacgccgg agggagacag
     5701 gtatcttccc agcgagaata tctccggtga gccggaggaa cggttccgga tggctccgga
     5761 ggactggctg cgggcacaaa tgcagggtga gattgtggca ctggtccaca gtcatcccgg
     5821 tggtctgccc tggctgagtg aggctgaccg gcggctgcag gtgcagagtg atttgccgtg
     5881 gtggctggtc tgccggggga cgattcacaa gttccgctgt gtgccgcatc tcaccgggcg
     5941 gcgctttgag cacggggtga cggactgtta cacgctgttc cgggatgctt atcatctggc
     6001 ggggattgag atgccggatt ttcatcgcgg ggatgactgg tggcgtcacg gtcagaatct
     6061 ctatctggat aatctggagg ccacagggct gtatcaggtg ccgttgtcat cagcacaacc
     6121 gggcgatgtg ctgctgtgct gttttggttc atcggtgccg aatcatgccg ccatttactg
     6181 cggcgatggt gagctgctgc accatattcc tgaacaactg agtaaacgag agaggtatac
     6241 cgacaaatgg cagcgacgca cacactccct ctggcgtcac cgggaatggc acgcatctgc
     6301 ctttacgggg atttgcaacg atttggccgc cgcatcgacc ttcgtgtgaa aacgggggct
     6361 gaagccatcc gcgcactggc cacacagctc ccggcgtttc gtcagaaact gagtgacggc
     6421 tggtatcagg tgcgcattgc cgggcgtgat gcaggtgaaa ccgaattgtc tgcccgtctt
     6481 aatgagccgc tggcaaatgg tgcagtgatc cacatagtac cgcgtctggt gggagctaaa
     6541 agtggcggtg tgtttcaggc ggtgctgggg gcagctgtta tggcggttgc tatatggatg
     6601 ccgggggtag gaattatggc gagtaatctg ctgttttctc tcggtgccag tatgacgctt
     6661 ggcggtgttg cacagatgct ggcaccgaaa gccagaactc cccgtacaca gacaacggat
     6721 aacggcaaac agaacacctg tttctcctca ctggataaca tggttgccca gggcaatgtt
     6781 ctgcctgttc tgtacggtga aatgcgcgtg gggtcgcggg tggtatctca ggagatcagc
     6841 acggcagacg aaggggatgg tggtcaggtt gtggtgattg gtcgctgatg caaaatgttt
     6901 catgtgaaac cgcctgcggg cggttttgtc gtttatggag cgtgaggaat gggtaaaggc
     6961 agcagtaagg ggcatacccc gcgcgaagcg aaggacaacc tgaaatccac gcagttgctg
     7021 agtgtgattg atgccatcag cgaagggccg gttgacggtc cggtggatgg attaaaaagc
     7081 gtgctgctga acggtacgcc ggtcctggat agcgagggga agacaaactt ttccggtgtt
     7141 acggtggtgt tccgcgccgg cgagcaggag cagacaccgc cggaggggtt tgaatcttcc
     7201 ggctcagaga ctgtgctggg tacggaagtg aaatacgaca cgccgatcac ccggaccatc
     7261 acgtcggcaa acattgatcg tctgcgcctg accttcggtg tgcaggcact ggtggaaacc
     7321 acctcaaagg gggaccggaa tccgtcggaa gtccgcctgc tggttcagat acagcgtaac
     7381 ggtggctggg tgacggaaaa agacatcacc attaagggca aaaccacttc gcagtatctg
     7441 gcctcggtgg tggtgggtaa cctgccgccg cgcccgttta atatccggat gcgcaggatg
     7501 acaccggaca gcaccacaga ccagctgcag aacaaaacgc tctggtcgtc gtacaccgaa
     7561 atcatcgatg tgaaacagtg ctacccgaac acggcgctgg tcggcgtgca ggtggattca
     7621 gagcagttcg gtaaccagca ggtgagtcgc aattatcatc ttcgcgggcg cattctgcag
     7681 gtgccgtcga actataaccc gcagacgcgg caatacagcg gtatctggga cggaacgctt
     7741 aagccagcat acagcaacaa catggcctgg tgtctgtggg acatgctcac tcatccgcgc
     7801 tacggcatgg ggaaacgtct tggtgcggca gatgtggaca aatgggcgct gtatgtcatc
     7861 ggccagtact gcgaccagtc agtgccggac ggatttggcg gcacggagcc gcgcatcacc
     7921 tgtaacgctt acctgaccac acagcgtaag gcgtgggatg ttctcagtga tttctgctcg
     7981 gcgatgcgct gtatgccggt atggaacggg cagacgctga cgttcgtgca ggaccgaccg
     8041 tcggataaag tgtggaccta taaccgcagt aatgtggtga tgccggatga tggcgcgccg
     8101 ttccgctaca gcttcagcgc cctgaaggac cgccataatg tcgttgaggt gaactggatt
     8161 gatccggaca acggtcatga gacggcgaca gagcttgtgg aggacacgca ggccattgtc
     8221 cgttacggtc gtaacgtcac gaagatggat gcctttggct gtaccagccg gggacaggcg
     8281 caccgcgccg ggctgtggct gattaaaacg gaactgctgg aaacgcagac cgtggacttc
     8341 agcgtcggcg cagaagggct tcgccatgta ccgggtgatg tcattgaaat ctgcgatgat
     8401 gattatgccg gtatcagcac cggtggtcgc gtactggcgg tgaacagcca gacccggacg
     8461 ctgacgctcg accgtgaaat cacgctgccg tcctccggta ccacgctgat aagcctggtt
     8521 gacggaaatg gcaatccggt cagcgtggag gttcagtccg tcaccgacgg cgtgaaggtg
     8581 aaagtgagcc gggttcctga cggcgttgca gaatacagcg tgtgggggct gaagctgccg
     8641 acgctgcgcc agcgcctgtt ccgctgcgtg agtatccgtg agaacgacga cggcacgtat
     8701 gccatcaccg ccgtgcagca tgtgccggaa aaagaggcca tcgtggataa cggggcacac
     8761 tttgacggtg accggcgcgg cacggtgaat ggtgtcacgc cgccagcggt gcagcatctg
     8821 accgccgaag tcaccgcaga cagcggggaa tatcaggtgc tggcgcgatg ggacacgccg
     8881 aaggtggtga agggtgtgag cttcctgctt cgcctgaccg tggcagcgga cgacggcagt
     8941 gagcggctgg tcagcacagc caggacgacg gaaaccacat accgcttcag gcagctggcg
     9001 ctggggaatt acagtctgac agtccgggcg gtaaatgccc gggggcagca gggcgatccg
     9061 gcgtcggtat cgttccggat tgcggcaccg gcagcgcctg tcactattga actgataccg
     9121 gggtattttc agataacggt ggtcccgaaa ctggctgtat atgacccgac ggtgcagttt
     9181 gagttctggt tttcggaaaa gcggattgcg gatatcaggc aggttgaaac cagcgcccgc
     9241 tatcttggca cggcgctgta ctggatagcc gccagtatca atatcaggcc gggccatgat
     9301 tattattttt acgttcgcag tgtgaacacc gttggcaaat cggcatttgt ggaggccgtc
     9361 ggtcgggcga gcgatgatgc ggaaggttat ctgagttttt ataaagggtt gatcaataaa
     9421 acgcatctcg gcaaggagtt gtggacgcag attgataacg gtcagcttgc gccggacctg
     9481 actgaaatca ggacgtccat tacgaatgtc agcaatgaaa tcacgcaaac cgtcaataaa
     9541 aaactggaaa atcagagtgc tgcaatccag cagatacaga aagttcaggt tgatacaaat
     9601 aataacctga acagcatgtg ggctgtgaag ctgcaacaga tgaaggacgg acgcctttat
     9661 attgcgggta tcggtgccgg tattgagaat acgccagcag gaatgcagag tcaggtgctg
     9721 ctggcggcag acaggattgc gatgattaat cctgcgaatg gcaacacaaa gccgatgttt
     9781 gttggtcagg gcgatcagat attcatgaac gacgtgttcc tgaaacgcct gacggctccg
     9841 accattacca gcggcggtaa tcctccggca ttttccctga cacctggcgg acggctgacg
     9901 gcgaaaaatg ccgatatcag cggtaacgtg aacgcgaact ccgggacgct caacaacgtc
     9961 acgattaata agaactgtcg ggttctggga aaattgtccg cgaaccagat tgaaggcgat
    10021 ctcgttaaaa cagtgggcaa acctttccca cgggactccc gggcaccgga gaggtggcca
    10081 tcagggacca ttaccgtcag ggtttatgac gttcagccgt ttgatcggca aattgttatt
    10141 cccgcggtgg cgtttcgcgg tgctaaacat gagcggaaga ataacaatat ttattcgtca
    10201 tgccgcctga tagtgaagaa aaacggtgct gaaatttata accgaacgac cctggataat
    10261 acgctgatat atacgggtgt tattgatatg cctgccggtc acggtcacat gacgctggag
    10321 ttttctgtat cggcatggct ggtaaatggc tggtatccca cagcaagtat cagcgatttg
    10381 ctggttgttg tgatgaagaa atccacagca ggtatcagta tcagctgaat tttataaccc
    10441 agatacgggc accagaaatg gtgccttttt tattgcagaa aagcgagagg taattatgcg
    10501 taaactttat gctgccattt tgtccgcagc catctgtctg gctgtatccg gtgcgcctgc
    10561 atgggcgtct gaacatcggt ccacgctgag cgcggggtat cttcatgcct cgacgaacgc
    10621 tcccggcagt gatgatctga acgggattaa cgtgaaatac cgttatgagt ttacggacac
    10681 gctggggctg gtgacgtcat tcagctatgc caacgctgaa gatgagcaaa aaacgcatta
    10741 cagtgatacc cgctggcatg aggattccgt gcgtaaccgc tggttcagcg tgatggcggg
    10801 gctgtctgtg cgcgtgaatg aatggttcag cgcgtatgcg atggcgggcg tggcttacag
    10861 ccgtgtgtcg accttctccg gggattatct ccgcgtaact gacaacaagg ggaaaacgca
    10921 cgatgtgctg accgggagtg atgacaaccg ccacagcaac acgtctctgg cgtggggggc
    10981 tggcgtgcag tttaacccga ccgaatccgt ggccattgac cttgcttatg aaggttccgg
    11041 cagtggcgac tggcgcactg acggtttcat cgtgggtgtc ggttataagt tctgattagc
    11101 caggtaacac agtgttatga cagcccgctg gttcaggcgg gctttttgtg gggtgaatat
    11161 ggcagtaaag atttcaggtg tactgaaaga tggggcgggt aaacctgtcg taaattgtgc
    11221 gattgaactg cgggccagaa gaaccagtcc gaccgttgtg gcacacgttg ttgccacttg
    11281 cgtgacggac aataacggtg cttatgtgat tgaggctgag ccggggtatt acgaggttgc
    11341 gcttcactgt aacggctggc agccaacccg tgtcggggat attgatgtgg caccgactga
    11401 tgcaccgggg acactgaacg cgtttctgaa tgcaccaaag gatggtgatt tacgtccgga
    11461 ggtgatgaag cgctttgagg aaatggtggc gcaggcgcag cagagtgccg gggctgcagc
    11521 cggaaacgca cagcagacgg cgcaggatgt ggcggcagcc gcaacggccc gtgatgatgc
    11581 acaacgtttt gcggagaaag cccgacagga tgcaaccgtc acagctgagg acagaaaggc
    11641 cactgcggaa gatgtgacaa gcacaggagc aaatgcagcc gcagccggac agagcgcaca
    11701 ggatgccgca ggttatgccc gcgcagcaga acaggccaaa aatgacattg atgctgcgct
    11761 gaccggcact ctgaaaatgg cta
//
LOCUS       DAJTSI010000125        11655 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000125 DAJTSI010000000
VERSION     DAJTSI010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.125"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            597..794
                     /locus_tag="OO327_003071"
     CDS             597..794
                     /locus_tag="OO327_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019521.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="OO327_003072"
     CDS             complement(744..1937)
                     /locus_tag="OO327_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6019522.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="OO327_003073"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="OO327_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR6019523.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="OO327_003074"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="OO327_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR6019524.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="OO327_003075"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="OO327_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR6019525.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="OO327_003076"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="OO327_003076"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR6019526.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="OO327_003077"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="OO327_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR6019527.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10573..10716)
                     /locus_tag="OO327_003078"
     CDS             complement(10573..10716)
                     /locus_tag="OO327_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019528.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10955..>11655
                     /locus_tag="OO327_003079"
     CDS             10955..>11655
                     /locus_tag="OO327_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6019529.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQW"
BASE COUNT         2956 a         2897 c         2992 g         2810 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgcccagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctaccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcataaagt ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc
    10501 cggcccggag gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg
    10561 ctataggcaa agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt
    10621 ctgaataacg cagtgatgga gaacttcttc ggcacactgc aaaatcgcat cgtggtcgat
    10681 cgctaattcg tcgtgaatca ctgaacgatg ttccatgcga acagatattc gatcatatcg
    10741 aaatattaaa cattacccct ccccacattg ggcaaaaaaa aacaaaaaac gatcaattag
    10801 tattgattca cataatctct catcattaat gagaacattg tcacatattt acaatataga
    10861 actgttactt ttagcaagct gttagtaacc aaccgttact aataatttta attcttaata
    10921 ctttgttcat tcaataaatg agttgagaga atatatgaat aaaatatact cccttaaata
    10981 tagtgctgcc actggcggac tcattgctgt ttctgaatta gcgaaaagag tttctggtaa
    11041 aacaaaccga aaacttgtag caacaatgtt gtctctggct gttgcaggta cagtaaatgc
    11101 agcaaatatt gatatatcaa atgtatgggc gagagactat cttgatcttg cacaaaataa
    11161 aggtattttc cagcccggag caacagacgt aacaatcact ttaaaaaacg gagataaatt
    11221 ctctttccat aatctctcaa ttccggattt ttctggtgca gcagcgagtg gcgcagctac
    11281 cgcaatagga ggttcttata gtgttactgt tgcacataac aaaaagaacc ctcaggccgc
    11341 agaaactcag gtttacgctc agtcttctta caaggttgtt gacagaagaa attccaatga
    11401 ttttgagatt cagaggttaa ataaatttgt tgtggaaaca gtaggtgcca ccccggcaga
    11461 gaccaaccct acaacatatt ctgatgcatt agaacgctac ggtatagtca cttctgacgg
    11521 ttcaaaaaaa atcataggtt ttcgtgctgg ctctggagga acatcattta ttaatggtga
    11581 atccaaaatc tcaacaaatt cagcatatag ccatgacctg ttaagtgcta gtctatttga
    11641 ggtcacccaa tggga
//
LOCUS       DAJTSI010000126        11521 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000126 DAJTSI010000000
VERSION     DAJTSI010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.126"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(217..292)
                     /locus_tag="OO327_003080"
     tRNA            complement(217..292)
                     /locus_tag="OO327_003080"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(257..259),aa:Ile,seq:cat)
     gene            851..3103
                     /locus_tag="OO327_003081"
     CDS             851..3103
                     /locus_tag="OO327_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019530.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGPTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            3439..4371
                     /gene="csiD"
                     /locus_tag="OO327_003082"
     CDS             3439..4371
                     /gene="csiD"
                     /locus_tag="OO327_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCR6019531.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            4391..5659
                     /gene="lhgO"
                     /locus_tag="OO327_003083"
     CDS             4391..5659
                     /gene="lhgO"
                     /locus_tag="OO327_003083"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCR6019532.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5682..7130
                     /gene="gabD"
                     /locus_tag="OO327_003084"
                     /pseudo
     CDS             5682..7130
                     /gene="gabD"
                     /locus_tag="OO327_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            7138..8424
                     /gene="gabT"
                     /locus_tag="OO327_003085"
                     /pseudo
     CDS             7138..8424
                     /gene="gabT"
                     /locus_tag="OO327_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            8662..10062
                     /gene="gabP"
                     /locus_tag="OO327_003086"
     CDS             8662..10062
                     /gene="gabP"
                     /locus_tag="OO327_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCR6019533.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            10083..10745
                     /gene="csiR"
                     /locus_tag="OO327_003087"
     CDS             10083..10745
                     /gene="csiR"
                     /locus_tag="OO327_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCR6019534.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10746..11195)
                     /gene="kbp"
                     /locus_tag="OO327_003088"
     CDS             complement(10746..11195)
                     /gene="kbp"
                     /locus_tag="OO327_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR6019535.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(11279..11437)
                     /locus_tag="OO327_003089"
     CDS             complement(11279..11437)
                     /locus_tag="OO327_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR6019536.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2961 a         2830 c         2874 g         2856 t
ORIGIN      
        1 tttgcccgct tggcatagcg ggcatttttt ccaggtactt ttgaatgagt actgatgaat
       61 aaatacattg cagtggcgtg ccacgtacca aaacaccagc cctcattcga aaccacccac
      121 cgcacttctt ccttgaaatg gcgttagtca tgaaatatag accgccatcg agtacccctt
      181 gtacccttaa ctcttcctga tacatgaatg atgatttggt ggcccttgct ggacttgaac
      241 cagcgaccaa gcgattatga gtcgcctgct ctaaccactg agctaaaggg ccttaagtat
      301 gcaataacaa tacttataaa ccacgcaata aacatgatga tcttatgatg taaataacag
      361 atttttatgc gttcccattc accttgggtc gtgattacac gcgacataaa acccgacacc
      421 gcctccattc gcaaagtcga tactcgcagt caacaagcaa atgttaataa ttagcactat
      481 ctatagttat catcgattca atgatacttt gtaatgattt tgtatctaat aatataactt
      541 tattacatta gctgaagagt tttcgcatca ttatgatatc tgttactttt cactccataa
      601 aaataaactt catatagcaa tatattcttt catagatctt attctgctaa tcattagttt
      661 cgtatgcgcg atttttgaca gttgcctctc cagaccacat cgataattaa aaaacagatt
      721 taaacattat ccttttccat atagatattg gataaaaata ggacatctgt ttgcaattac
      781 tttcacaaca attaaacatt tttatgtttt cgtatacatc atattactct accattagag
      841 gaacgttatt atgttttcta taaaaccagg acctggaaat ttacctatcg acaaccccac
      901 attgttatca tggaacatta ctgatgggga tctaaattcc aaatttaata cattagaata
      961 tctaaactgt ataacaaata ttattaatgc ttgtggagtt tatcctcagg atttaaaaga
     1021 cagagaaatt atatcaactt ttcacgcaga aaaagtcatt aatgatctgt taaaaaacga
     1081 ttataaaatt tccctttctc cagatacaac ttaccgagag ctgaataaag ctgcacagag
     1141 tagcattaca gcgccagaca ggataggaga agggaaaaca tgggtttatc aacgagatac
     1201 aatggttgaa agaggtgata acagtggtgt tcatcagtat ggtccagctg aacatttcac
     1261 tcacattata tctgacaaac cgtccccaaa agataagtat gttgcatatg ctattaacat
     1321 tcctgactat gagctagcag ccgatgtata taatattaac gtgacgtcac cttccggaca
     1381 gcaagaaaca tttaaaatac taatcaatcc agaacatcta cggcaaacac ttgagcgtaa
     1441 atctcttact gctgttcaga aatcacaatg tgaaatcatc acccccaaaa aacctggcga
     1501 agcgattctt catgctttta atgccaccta ccagcaaatc agggaaaata tgtctgagtt
     1561 tgcacgttcc cattatgggt atatacaaat ccctccagtg acaactttcc gcgccgacgg
     1621 accagaaact cccgaagaag aaaaaggtta ctggtttcac gcttatcaac ccgaagatct
     1681 ttgtaccatc cataatccaa tgggggattt gcaggatttt atcgcattgg ttaaagatgc
     1741 taaaaaattt ggtatcgata tcattcctga ttataccttt aactttatgg gaatcggggg
     1801 aagtggtaaa aatgacctgg attatccctc tgctgatata cgagcgaaga tcagtaaaga
     1861 tatagaaagt ggtatccctg gctattggca aggtcaggtt ttgattccat tcattaaaga
     1921 tccagtaaca aaagaacgta aacaaatcca tccagaagat atacatctca ctgcaaaaga
     1981 cttcgaagca agtaaagata acatctctaa ggatgaatgg aaaaacctcc atgcattaaa
     2041 agaaaagagt ttaaatggaa tgcctaaaac aacccccaaa agtgaccagg ttattatgtt
     2101 gcaaaatcaa tacgttcgtg aaatgcgaaa atatggcgta cgaggtttac gttatgatgc
     2161 agcaaaacac tcaaaacatg aacaaataga aagatcaata accccaccgc ttaaaaatta
     2221 taatgagcga ttacacaata ctaacttatt taacccaaaa tatcatgaaa aaaccgttat
     2281 gaattacatg gaatatctgg taacttgtca gttgaatgaa gaacaaatgt catcgctgct
     2341 ttatgaaaga gatgatttaa gcgccattga tttttcattg ctcatgaaga cgataaaagc
     2401 cttttcattc gggggagatc tcctaaccct tgcatcaaaa ccgggttcca caatctcaag
     2461 catcccatca gaaagacgga tattgattaa cattaaccac gattttccta acaatggcaa
     2521 tcttttcaat gactttctat ttaaccatca acaagatgaa caattagcaa tggcatatat
     2581 ggccgctctc ccgttcagca ggcctttagt ttactgggat ggccaggtat taaaatcaac
     2641 gactgaaatt aaaaattatg atgggcccac gcgtgtcggt ggtgaggcgt ggcttaataa
     2701 aggttgctct acctatcagc agctctacaa tgaattccac gcattatata tagataaagc
     2761 aggaatatgg agcgcatttg agggtgtatt tgcaactaaa aacgttctgg cctttagtcg
     2821 tggggattct gtgaacatta atcactctcc tcatgatgga ctagttataa taaataaagg
     2881 aaacgaagaa gttgaaggta cctggcctaa caaattgcaa cctggaatat ataaaaacat
     2941 ggggagtaat agcgttaaca ttattattaa taatacccga aaaattatcc ccccttgtaa
     3001 agtatttacg cttagaggcg gatctctaaa tatcaatatt cctaggcgta gcgctcttct
     3061 tttagggaaa acaggagaac cgccgaacta tctctattta taatttaatg ttatatctgc
     3121 cccgataaaa cggggcagat aatatgttta gtttactaac ggtcattttg tagtgaagcc
     3181 atttactgtt ttttatcgac cagataatct gttctctaat gttaactccc cctaacctgt
     3241 tgctttagtt attcatttcc tgtctcactt tgccttaata ccctacgtta aatgttacta
     3301 atttgttgct tttgatcaca ataagaaagc aatatgtcgc ttttgtgcgc atttttcaga
     3361 aatgtagata tttttagatt atggctacga aatgagcatc gccatgtcac cctacatctc
     3421 ataagaggat cgcttctgat gaatgcactg accgccgtac aaaataacgc tgtcgattca
     3481 ggccaggact atagcggatt cactctcatc ccgtcggcgc aatccccgcg tctgctggaa
     3541 ctcaccttca ccgaacagac gaccaaacag tttctcgaac aggttgccga atggcccgtg
     3601 caggcgctgg agtacaaatc gtttctgcgt tttcgggtag gcaaaattct tgacgatctg
     3661 tgtgcgaatc agctgcaacc attgctgttg aagaccctgc taaaccgtgc tgaaggtgcg
     3721 ctgttgatca atgcggtagg tatcgatgat gtggcgcagg cagatgagat ggtgaagctg
     3781 gcaacggcgg tagcgcatct gattggtcgc tcaaatttcg atgcgatgag cggtcagtat
     3841 tacgcgcgtt tcgtggtgaa aaatgtcgat aactcagaca gctatctgcg tcagccgcac
     3901 cgcgtgatgg aactgcacaa cgacggcacg tatgtcgaag agatcaccga ttacgtgctg
     3961 atgatgaaaa tcgatgactg ggaacatctg gaccactatt tccgccatcc actggcgcgt
     4021 cgcccgatgc gctttgccgc gccgccgagc aaaaacgtca gcaaagatgt tttccatcca
     4081 gtgttcgacg ttgatcaaca gggtcgcccg gtgatgcgct atatcgacca gttcgtccag
     4141 ccaaaagatt ttgaagaagg cgtgtggttg agcgagcttt ccgatgccat tgaaaccagc
     4201 aaaggcattc tttctgtacc cgttcccgtt ggcaaattcc tgttgataaa caacctgttc
     4261 tggctgcacg gtcgcgaccg ctttactccg cacccggatc tgcgccgtga actgatgcgt
     4321 cagcgtggct atttcgctta cgccactcac cactaccaga cgcatcagta agcgcaaagg
     4381 aattgagcgg atgtatgatt ttgtgattat tggcggcggc atcatcggca tgtcgaccgc
     4441 catgcaactg attgatgttt atccggatgc ccgcattgcg ttgctggaaa aagagtccgg
     4501 cccggcctgt caccagacgg gccacaacag cggcgtgatc catgccgggg tctattacac
     4561 gcccggcagc ctgaaggcgc agttttgcct ggcggggaac cgtgccacca aagccttttg
     4621 cgatcaaaac ggcattcgct atgacaactg cggcaagatg ctggtcgcca cgtctcagct
     4681 cgaaatggaa cggatgcgcg ccttatggga acgcacagcg gcgaacggta tcgagcgcga
     4741 gtggataaac gccgtggaac tgcgcgagcg cgaaccgaat atcaccgggc ttggtggcat
     4801 ttttgtgccg tccagcggca ttgtcagcta ccgcgaagtg acggcggcaa tggcaaaaat
     4861 cttccaggcc agaggcggcg agattattta taacgccgaa gtcagcgccc tcagtgagca
     4921 taaaaacggc gtggtgatac gcacccgtca gggcggtgaa tatgaagcat cgacgctgat
     4981 tagctgttcc gggctgatgg ctgaccggct ggtgaaaatg ctcggcctcg aaccgggctt
     5041 tattatctgc ccgttccgtg gcgagtattt ccgccttgcg ccggagcata accagattgt
     5101 taaccacctg atttacccca ttcccgaccc cgcgatgcca tttttgggcg tgcatctcac
     5161 ccgcatgatc gacggcagcg tgaccgtcgg gccaaacgcg gtgctggctt tcaaacgcga
     5221 aggctatcgc aagcgcgact tctcatttag tgacacgctg gagattttga gctcgtcggg
     5281 gattcgccgg gtgctgcaaa accatctacg ctcaggactg ggcgagatga aaaactcgct
     5341 gtgcaaaagc ggctatctgc ggctggtgca aaagtattgt ccccggcttt cgttaagcga
     5401 tctccagccc tggcccgccg gtgtgcgggc gcaggcagta tcgccggacg gcaagctgat
     5461 tgacgatttt ctgtttgtca ccaccccacg cacgatccac acctgcaatg cgccctcccc
     5521 ggcagcgaca tcagcaattc ctattggtgc gcatattgtc agcaaggtac aaacgctgtt
     5581 ggcaagccag agtaaccccg gacgcacgct gcgagcggca cgtagtgtgg atgccttaca
     5641 cgccgcattt aatcaataac ctttgaaaac aggacgtaac aatgaaactt aacgacagta
     5701 acttattccg ccagcaggcg ttgattaacg gggaatggct ggacgccaac aatggtgaag
     5761 tcatcgacgt caccaatccg gcgaacggcg acaagctggg tagcgtgccg aaaatgggcg
     5821 cagatgaaac ccgcgccgct atcgacgccg ccaaccgcgc cctgcccgcc tggcgtgcgc
     5881 tcaccgccaa agaacgcgcc accattctgc gcaactggtt caatttgatg atgcagcatc
     5941 aggacgattt agcgcgcctg atgaccctcg aacagggtaa accgctggct gaagcgaaag
     6001 gcgaaatcag ctacgccgcc tccttcattg agtggtttgc tgaagaaggc aaacgtattt
     6061 atggcgacac cattcctggt catcaggccg ataagcgcct gattgttatc aagcagccga
     6121 ttggcgtcac cgccgccatc acgccgtgga actttccggc ggcgatgatt acccgtaaag
     6181 ccggtccggc gctggcggca ggctgcacca tggtgctgaa gcccgccagt cagacgccgt
     6241 tctctgcgct ggcgctggcg gaactggcga ttcgcgcggg cattccggct ggcgtgttta
     6301 acgtggtcac cggttcggca ggcgtggtcg gtaatgaact gaccagcaac ccgctggtac
     6361 gcaaactgtc gtttaccggt tagaccgaga ttggccgcca gttaatggaa cagtgcgcga
     6421 aagacatcaa aaaagtgtcg ctggagctgg gcggcaacgc gccgtttatc gtctttgacg
     6481 atgccgatct cgataaagcc gtggaaggcg cgctggcctc gaaattccgc aacgccgggc
     6541 aaacctgcgt ctgcgccaac cgtttatacg tgcaggacgg cgtgtatgac cgctttgccg
     6601 aaaaattaca gcaggcgggg agcaaactgc acatcggcga cgggctggag aaaggcgtca
     6661 ccatcgggcc gctgatcgat gaaaaagcgg tagcaaaagt ggaagagcat attgccgatg
     6721 cgctggagaa aggtgcgcgt gtggtttgcg gcggtaaagc gcacgaacgc ggcggcaact
     6781 tcttccagcc aaccattctg gtggacgttc cggccaacgc caaagtgtcg aaagaagaga
     6841 cgttcggccc cctcgcccca ctgttccgtt ttaaagatga agccgatgtg attgcacaag
     6901 ccaatgacac cgagtttggt cttgccgcct atttctacgc ccgtgattta agccgcgtct
     6961 tccgcgtcgg cgaggcgctg gagtacggca tcgtcggcat caataccggc attatttcca
     7021 atgaagtggc cccattcggc ggcatcaaag cctcgggtct gggtcgtgaa ggttcgaagt
     7081 atggcatcga agattactta gaaatcaaat atatgtgcat cggtctttaa ctggagaatg
     7141 cgaaggaaca gcaataaaga gttaatgcag cgccgcagtc aggcgattcc ccgtggcgtt
     7201 gggcaaattc acccgatttt cgctgaccgc gcggaaaact gccgggtgtg ggacgtttaa
     7261 ggccgtgagt atcttgattt cgcgggcggg attgcggtgc tcaataccgg gcacctgcat
     7321 ccgaaggtgg tggccgcggt ggaagcgcag ttgaaaaaac tgtcgcacac ctgcttccag
     7381 gtgctggctt acgagccgta tctggagctg tgcgagatta tgaatcagaa ggtgccgggc
     7441 gatttcgcca agaaaacgct gctggttacg accggttccg aagcggtgga aaacgcggta
     7501 aaaatcgccc gcgccgccac caaacgtagc ggcaccatcg cttttagcgg cgagtatcac
     7561 gggcgcacgc attacacgct ggcgctgacc ggcaaggtga atccgtactc tgcgggcatg
     7621 gggctgatgc cgggtcatgt ttatcgcgcg ctttatcctt gcccgctgca cggcataagc
     7681 gaggatgacg ctatcgccag catccaccgg atcttcaaaa atgatgccgc gccggaagat
     7741 atcgccgcca tcatgattga gccggttcag ggcgaaggcg gtttctacgc cgcgtcgcca
     7801 gcctttatgc agcgtttacg cgctctgtgt gacgagcacg ggatcatgct gattgccgat
     7861 gaagtgcaga gcggcgcggg gcgtaccggc acgctgtttg cgatggagca gatgggcgtg
     7921 gcgccggatc ttaccacctt tgcgaaatcg atcgcaggcg gtttcccgct ggcgggcgtc
     7981 accgggcgcg cggaagtgat ggacgctgtt gcaccgggcg gtctgggcgg cacctatgcc
     8041 ggtaacccga ttgcctgcgt ggctgcgctg gaagtgttga aggtgttcga gcaggaaaat
     8101 ctgctgcaaa aagccaacga tctggggcag aagctaaaag acggattgtt ggcgatagcc
     8161 gaaaaacacc cggagatcgg cgacgtacgc gggctgggat cgatgattgc catcgagctg
     8221 tttgaagatg gcgatcacag caaaccggac gcaaaactca ccgccgagat cgtggcacgc
     8281 gcccgcgata agggcctgat tcttctctcc tgcggcccgt attacaacgt gctgcgcatc
     8341 cttgtaccac tcaccattga agacgctcag atccgtcagg gtctggagat tatcggtcag
     8401 tgttttgatg aggcgaagca gtagcaccgt ccccatgcag gaggcgacgc tgcgcgtctt
     8461 gtccggccta cgagaatcca agtcggataa ggcgtttacg ccgcatccga caatctgtac
     8521 gtgaacagga ataaatctat gtcggccgga taaggcggag ccgctctccg gcaaaaagaa
     8581 tcaataacaa ttaaacgcgt gacccgacgc gggaaatgtc ggggcgctct cccaagtgac
     8641 acactttcga gaggattcag gatggggcaa tcatcgcaac cacacgagtt aggcggcggg
     8701 ctgaaatcac gccacgtcac catgttgtct attgccggtg ttatcggcgc aagtctgttt
     8761 gtcggttcca gcgtcgccat cgccgaagcg gacccggcgg tattactggc ctatctgttc
     8821 gccggattac tggtggttat gattatgcgg atgctggcgg aaatggcagt tgccacgccc
     8881 gataccggtt cgttttccac ctatgccgat aaagccattg gccgctgggc gggctatacc
     8941 atcggctggt tgtactggtg gttttgggta ctggttatcc cactggaagc caacatcgcc
     9001 gccatgatcc tgcactcgtg ggttccaggc attcccatct ggttattttc cctcgtcatt
     9061 accctcgcct taactggcag taatttatta agcgttaaaa actacggcga atttgagttc
     9121 tggctggcgc tgtgcaaagt catcgctatc ctggccttta ttttccttgg tgcagtcgca
     9181 attagcggtt tttaccctta tgccgaagtg agcgggatct caagattgtg ggatagcggc
     9241 ggctttatgc ccaacggttt cggtgcggta ttaagcgcga tgttgatcac catgttctcg
     9301 tttatgggcg cagaaattgt caccattgcc gccgcggaat ccgacatgcc ggaaaaacat
     9361 attgtccgcg ccaccaactc ggttatctgg cgtatttcta tcttctattt gtgctctatt
     9421 tttgtcgtcg tggcattaat accgtggaat atgccggggc tgaaagccgt tggatcttat
     9481 cgctcggttc tggaattgct caatattccc catgcgaaat taatcatgga ctgtgtgata
     9541 ttactttccg taaccagttg cctgaactcg gccctgtata ccgcctcaag gatgctctac
     9601 tccttaagcc gtcgcggtga tgctcccgcg gtaatgggta aaatcaaccg cagtaaaacc
     9661 ccgtacgtgg cggtgttact ctccaccggc gcagcattct taacggtggt ggtgaactat
     9721 tacgcgcctg cgaaggtatt taaatttctg atcgacagct ccggcgctat cgccctgctg
     9781 gtttatttag tcatcgccgt ttcacagttg cggatgcgca aaattctgcg agcagaagga
     9841 agcgaaattc gcttgcgaat gtggcttgat ccctggctca cctggctggt catcggcttt
     9901 attacctttg tgttggtagt gatgctattc cgtccggcgc aacagttaga agtgatctcc
     9961 accggcttat tagcgatagg gattatctgt accgtgccaa ttatggctcg ctggaaaaag
    10021 ctggtattgt ggcaaaaaac acccgttcat aatacgcgct gataatgatc aggagtcaca
    10081 ccatgaccat tacgtctctg gatggctatc gctggctgaa gaacgatatt attcgcggta
    10141 attttcaacc ggatgaaaaa ttacggatga gtttactgac atcgcgttat gcacttggcg
    10201 ttgggccgtt gcaggaagca ctttctcaac tggtggcgga acggctggtc acggtggtga
    10261 atcaaaaagg gtatcgggtg gcgtctatgt cagagcagga gctgctcgat attttcgacg
    10321 cccgcgccaa tatggaagcg atgctggtca gtctggcgat tgcccgcggt ggcgatgaat
    10381 gggaggcaga cgttctcgca aaagcgcatc tgctcagtaa gctggaagcc tgtgacgcca
    10441 gcgagaaaat gcttgatgag tgggatctgc gtcatcaggc gtttcataca gcaattgtgg
    10501 cgggctgtgg ttctcactat ttgctgcaaa tgcgtgaacg gttgtttgat ctggcggcgc
    10561 gttatcgatt tatctggctg cggcgaacgg tgctttcggt ggaaatgctg gaggataaac
    10621 acgatcagca ccagaccctg actgcggcgg tactgccgcg agatacagcg cgcgccagtg
    10681 agttaatgcg ccagcattta ctgacgccaa ttcccattat ccagcaggcg atggctggca
    10741 attaactact cttccggaat acgcaacact tgccccggat aaattttatc cgggcttttt
    10801 agcatcggtt tattcgcttc gaagatttta ttgtacagat tagcgttacc gtagacctgt
    10861 ttggaaatgg cactcagagt gtcgccagac ttaacggtat aaaactggct ggcagtggct
    10921 ggtgtcgccg ttttcacctg atcatcaaca ctggcaatac cggaaatatt ccccaccgca
    10981 acaaggattt tctccttcgc ctcctgactc aggccgtcac cagtgaccgt cgctttgccg
    11041 tcggcaattt gaatgttcac tttatcggca tcaggtatac cggttttgct cagatgctcc
    11101 tgcaccttct tcgcctgatc gtctttatcg tgctgacctg taaccgcatc ccagagtttt
    11161 tctccggcat ctttaacaaa attgaacaga cccatagcta cctcattcgt taacggaaac
    11221 actcagaaag tgtagcagag gaacgcggcg cgttaacgcc gcgggggtga gcacaggttt
    11281 aatcgcgggt ttgcacccag aacgcgtgaa tcaaaccggg gatataccca agcagtgtca
    11341 gcagaatatt aataatgaac gcccaaccga accctttacc gagcagcacg ccgatcggcg
    11401 gcagaatgat ggtgatgacg attctccaga aacccatatg tactccctat aagaaaatta
    11461 ctcattgttt aaaaagagat tttatctctt aagcgtagta attttagcgg agcctgccag
    11521 t
//
LOCUS       DAJTSI010000127        11184 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000127 DAJTSI010000000
VERSION     DAJTSI010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.127"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..609)
                     /gene="hdhA"
                     /locus_tag="OO327_003090"
     CDS             complement(<1..609)
                     /gene="hdhA"
                     /locus_tag="OO327_003090"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCR6019537.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNSIAPGAILTDALKSVITP"
     gene            complement(721..1749)
                     /gene="malI"
                     /locus_tag="OO327_003091"
     CDS             complement(721..1749)
                     /gene="malI"
                     /locus_tag="OO327_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR6019538.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1924..3516
                     /gene="malX"
                     /locus_tag="OO327_003092"
     CDS             1924..3516
                     /gene="malX"
                     /locus_tag="OO327_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR6019539.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3526..4698
                     /gene="malY"
                     /locus_tag="OO327_003093"
     CDS             3526..4698
                     /gene="malY"
                     /locus_tag="OO327_003093"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR6019540.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4802..5803
                     /gene="add"
                     /locus_tag="OO327_003094"
     CDS             4802..5803
                     /gene="add"
                     /locus_tag="OO327_003094"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR6019541.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5839..6879)
                     /locus_tag="OO327_003095"
     CDS             complement(5839..6879)
                     /locus_tag="OO327_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6019542.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            7122..7247
                     /gene="blr"
                     /locus_tag="OO327_003096"
     CDS             7122..7247
                     /gene="blr"
                     /locus_tag="OO327_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR6019543.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7520..7735
                     /gene="ydgT"
                     /locus_tag="OO327_003097"
     CDS             7520..7735
                     /gene="ydgT"
                     /locus_tag="OO327_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR6019544.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7821..8261
                     /locus_tag="OO327_003098"
     CDS             7821..8261
                     /locus_tag="OO327_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR6019545.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            8338..8919
                     /gene="rsxA"
                     /locus_tag="OO327_003099"
     CDS             8338..8919
                     /gene="rsxA"
                     /locus_tag="OO327_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR6019546.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8919..9497
                     /gene="rsxB"
                     /locus_tag="OO327_003100"
     CDS             8919..9497
                     /gene="rsxB"
                     /locus_tag="OO327_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR6019547.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9490..>11184
                     /gene="rsxC"
                     /locus_tag="OO327_003101"
     CDS             9490..>11184
                     /gene="rsxC"
                     /locus_tag="OO327_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR6019548.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIAR"
BASE COUNT         2598 a         2877 c         2817 g         2892 t
ORIGIN      
        1 tggtgtaata acggatttca gggcatcggt taatattgcc cccggcgcaa tgctattcac
       61 ccgaatattt ttttcaccca ggtcaaatgc catatttctg accagatgac tggccgcagc
      121 tttagatgat gcataggaag tcatgtttat atttttattt tctgccgcca tagaagtgat
      181 ggtcagaata acgccacctc catttttttc catttctggc gcaacaagtt gtgacagatg
      241 gaaaaaagaa aacacattca gttcataagc acggcgaaaa tctgccattg gcatatcaaa
      301 cggtttagga ccaccgccac cggcgttgtt aaccaggata tcaactttac ccagattact
      361 gacggcaaag tctgccagtg cagagagttc ctgttcggaa gtaatatcac aacgccaggc
      421 aaatgcctga ccatccagtt gttgaatttc gtctacaaca tggttagctg cgtcggcgtt
      481 aatatcactg accaccacag atgcgccagc tgtcgcgaat gtaatggcga tttctttacc
      541 aatacctgca cccgcacctg tgatgatggc gcattttccg tcgagtctca ggttgtcaga
      601 attaaacaca taaacctcct gtgaacttca gtaaaaataa aaggcaccgc ctgtgtgcca
      661 cattttttat cattaacctt gaggaaagga cactaccaag tatagcagat aaagagagga
      721 ttatttcgcc gcaatgagcc gggcgggaat aataagattg cgtgaatgcg tctcttcatg
      781 ggtgattttt tgcatcatgc gatccgcaag tgtgatacca agttcccgcg ctggcgtgct
      841 ggcccaggta acaggtatat catcaagtgt ggttggtgtc gcatcggtaa atgccgccag
      901 cgaaacctgt tgctcaaagt aacgatcgac tccgctttcc ccgctttgcc gccctgcttt
      961 cagcaaacca aaccatgccc ccatcgcaat agtttcgtta tagcaaacca cggcactgat
     1021 ggtcgggtta tgacgtaaaa gcgccgtgat agcttccgcg gcttgcttct ggctggaagt
     1081 gcactccaac acccaatcgc tgtgaaacgg caggccaaat tttagtagag ttgcacaata
     1141 gccccccacc cgttctgcac gggttaatga ggaactttgc cctcccagcc aggcgatccg
     1201 ctgatgccca ttgcgaatga gatgctccgt caacaactgt gcagcctgca tgttgtccgg
     1261 gcgaaccgta tcaacatcat caagataact ggcacgggaa gcgaaaatca ccgggatagc
     1321 tttttcttct gccatccgcc gcaggtcatc gctgcttcct gcagccccgg caattaccac
     1381 accatcgaca ccctgattca gtaacagtga aaaccgctgt gccagctgct cgccgtcttt
     1441 accgccgtga agcaaaaaaa ccatccgtcc ctgtgcttcc agagcttccg tcaatccggc
     1501 cgtcaattcg gcgtaaaacg gcgcagataa atcacggacg atcaaaccaa tgacgccgct
     1561 ttgcccgccg cgcagcgccg acgcctggcg attgcgcaca aatcccagct cttcaatggc
     1621 ggcgttaacg cgttctcctg tggcggtaga gattcgccct ttgccattaa gcaccagcga
     1681 aacggtactt accgacacgc ccgcagccag cgcaacatca tgaatggtta tttttttggc
     1741 ggtagccatg cagatgacct actccctgat tatgtgacag ataaaacgtt ttacctttta
     1801 tttatcttat acccgctatt atcgttgcgt aatgtgattt atgactcact aaaatttgat
     1861 aaaacgtttt atcttctcgc gcaatttact gaatccagat tgttctctac gaggagtcgt
     1921 tttatgacgg cgaaaacagc accgaaagtc acgctgtggg agttcttcca gcagttaggc
     1981 aaaaccttca tgttacccgt ggcattattg tcgttctgcg gcattatgct cggcattggt
     2041 agttctctta gcagccatga tgtcataacc ctgatcccgg tcctgggcaa ccccgtgttg
     2101 caggctatct ttacctggat gagtaagatt ggctcgtttg cttttagttt cctgcctgtc
     2161 atgttctgta tcgccatccc gctgggcctg gcacgcgaaa ataaaggcgt agcggcattc
     2221 gctggcttcg tcggttatgc ggtaatgaac ctcgcggtaa acttctggtt gaccaataaa
     2281 ggcattctgc caaccacaga tgccgccgtt ctgaaagcca ataacatcca gagcattctt
     2341 gggatccagt cgatcgatac cgggatcctc ggtgcggtga tcgccggtat tatcgtctgg
     2401 atgctgcatg agcgtttcca taatatccgc ctgccggatg cgctggcatt cttcggcggt
     2461 acgcgcttcg taccaattat ctcctcgctg gtgatgggcc ttgtcggcct ggtgattcca
     2521 ttagtctggc cgattttcgc catgggtatt agcggcttgg gccatatgat aaacagcgcg
     2581 ggtgatttcg gaccgatgct gtttggtacc ggtgaacgtc tgctgttgcc gtttggtctg
     2641 catcacattc tggtggcatt aattcgcttt accgacgcag gcggcacgca ggaggtctgc
     2701 ggtcaaaccg tcagcggcgc actgaccatc ttccaggcgc aattgagttg cccgaccact
     2761 cacggttttt ctgaaagcgt cacgcgtttc ctttcgcaag gtaaaatgcc tgcgtttctc
     2821 ggcggtctac caggtgcagc gttagctatg tatcactgcg cgcgcccgga aaatcgccat
     2881 aaaattaaag gtctgctgat ttctggcctg atcgcctgcg tcgttggcgg cactaccgaa
     2941 ccgctggaat tcctgttcct gttcgtagcg ccagttctgt atgtcatcca cgcgctgtta
     3001 accggcctcg gcttcaccgt catgtctgtg ctcggcgtca ccatcggtaa taccgacggc
     3061 aatatcatcg acttcgtggt gttcggtatt ttgcatggtc tgtcaaccaa gtggtacatg
     3121 gtgccagtgg tggcggcaat ctggtttgtc gtttactacg tcatcttccg tttcgctatc
     3181 acccgcttca atctgaaaac cccggggcgc gatagcgaag ttgccagctc aatcgaaaaa
     3241 gccgttgccg gtgcgccggg taaatcaggt tacaacgttc ctgcaatcct cgaagcatta
     3301 ggcggtgccg acaatattgt cagcctcgat aactgcatta cccgtctgcg tttgtctgtg
     3361 gaagatatgt cgcttgttaa tgtgcaggca ctgaaggaca atcgggcaat tggcgtagta
     3421 caacttaatc aacataacct gcaggttgtt atcgggccac aagttcagtc agtaaaagat
     3481 gaaatggccg gtctgatgca tactgtccag gcataaggat aagatatgtt cgatttttca
     3541 aaggtcgtgg atcgtcatgg cacatggtgt acacagtggg attatgtcgc tgaccgtttc
     3601 ggcactgctg acctgttacc gttcacgatt tcagacatgg attttgccac tgccccctgc
     3661 attatcgagg cgctgaatca gcgcctgatg cacggcgtat ttggctacag ccgctggaaa
     3721 aacgatgagt ttctcgcggc tattgcccac tggttttcca cccagcatta caccgcaatt
     3781 gatacgcaga cggtggtgta tggcccttct gtcatctata tggtttcaga actgattcgt
     3841 cagtggtctg aaacaggtga aggcgtggtg atccacacgc ccgcctatga cgcattttac
     3901 aaggccattg aaggtaacca gcgcacagta atgcccgttg ctttagagaa gcaggctgac
     3961 ggctggtttt gcgatatggg caagctggaa gccgttttgg cgaaaccaga atgtaaaatt
     4021 atgctcctgt gtaacccaca gaatcctacc gggaaagtgt ggacgtgcga tgagctggag
     4081 atcatggctg acctgtgcga gcgtcatgat gtgcgggtta tttccgatga aatccatatg
     4141 gatatggtct ggggcgagca gccgcatatt ccctggagta atgtggcgcg gggagactgg
     4201 gcgttgctga catcgggatc gaaaagtttc aatattcccg cgctgaccgg tgcttacggg
     4261 attatagaaa atagcagcag ccgtgatgcc tatttatcgg cactgaaagg acgtgatggg
     4321 ctttcttccc cttcggtact ggcgttaact gcccatatcg ccgcctatca gcaaggtgca
     4381 ccgtggctgg atgccttacg cgtctatctg aaagataacc tgacgtatat ctcagataaa
     4441 atgaacgccg cgtttcctga actcaactgg cagatcccgc aatccactta tctggcctgg
     4501 cttgatctac gtccgttgaa tattgacgac aacgcgttgc aaaaagcgct tatcgagcaa
     4561 gaaaaagtcg cgatcatgcc ggggtatacc tatggtgaag aaggtcgtgg ttttgtccgt
     4621 ctcaatgccg gctgcccacg ttcgaaactg gaaaaaggtg tggctggatt aataaacgcc
     4681 atccgcgctg ttcgctaacc ccaattgcgc aacgtaaaaa atcgttgcgc aatcgtggat
     4741 ttttaccctg ctttgttttt ataatggtgc gcacttttat atccagaaaa agagtgcgac
     4801 catgattgat accaccctgc cattaactga tatccatcgc caccttgatg gcaacattcg
     4861 tccccagacc attcttgaac ttggccgcca gtataatatc tcgcttcctg cacaatccct
     4921 tgaaacactg attccccacg ttcaggtcat tgccaacgaa cccgatctgg tgagctttct
     4981 gaccaaactt gactggggcg ttaaagttct cgcctctctt gatgcctgcc gccgcgtggc
     5041 atttgaaaac attgaagatg cagcccgtaa cggcctgcgc tatgtcgagc tgcgtttttc
     5101 accaggctac atggcaatgg cacataagct gcctgtagcg ggtgttgtcg aagcggtgat
     5161 cgatggcgta cgtgaaggtt gccgcacctt tggtgtgcag gcgaagctta tcggcattat
     5221 gagccgaacc ttcggcgaag ccgcctgtca gcaagagctg gaggcctttt tagcccaccg
     5281 tgaccagatt accgcacttg atttagctgg tgatgaactt ggattcccgg gaagtctgtt
     5341 cctttctcac ttcaaccgcg cgcgtgatgc gggctggcat attaccgtcc atgcaggcga
     5401 agctgccggg ccggaaagca tctggcaggc gattcgtgaa ctgggtgcgg agcgtattgg
     5461 acatggcgta aaagccattg aagatcgggc gctgatggat tttctcgccg agcaacaaat
     5521 tggtattgaa tcctgtctga cctccaatat tcagaccagc accgtagcag agctggctgc
     5581 acatccgctg aaaacgttcc ttgagcatgg cattcgtgcc agcattaaca ctgacgatcc
     5641 cggcgtacag ggagtggata tcattcacga atataccgtt gccgcgccag ctgctgggtt
     5701 atcccgtgag caaatccgcc aggcacagat taatggtctg gaaatggctt tcctcagcgc
     5761 agaggaaaaa cgcgcactgc gagaaaaagt cgccgcgaag taacaaaaat ggatggtgca
     5821 aattgcacca tccatttttc acgcaaggca caacgtcgcg cgatgtttgg cggattcgat
     5881 gcccagctca atcaactcca ttacctggat tgcctggctt gccggaaccg gattttcacc
     5941 atcgccattt aacgcatcac gaatcgccgc atagtaagcc ggataattcc caggcaccgt
     6001 caacagcgtt tcttcgacac gttcctcacc ttccacgcgg gtaagtacgc catcacgcat
     6061 atcgtagccc cagtcttcct gcggcagacg ctcgccattt ttcagacgtt cttcctgtgg
     6121 atcgaggcca tatttcacat aactgcctcg ggatccatgc acgatatacc gtgctgactc
     6181 agcggctgcc agcatggtac cgtgtaaaat gactcgccgc tgtggatagg acaagatggc
     6241 gtggaaataa tcggtcgact gcgctccggg ccgtaactgt gccaaatcta ccgtcatgct
     6301 gaccggtaaa ccaaatagcg taatggcctg atcaagaaga tgcggtgcta aatcgtacca
     6361 gataccgctg cctgggccgc cctgttcacg ccaacgatcg cgcacctgcg gacggaagcg
     6421 gtcaaaatga gactcaaagt aagcaacttc acccagcacg ccttccgcga gcaaaccttt
     6481 tagcgtcaag aaatcgctat cccaacggcg gttatggaat acagacagca cacgccccag
     6541 gctttttgcc agcgcatcca gctctcgcgc ttgtgacagt gtcacggtaa agggtttatc
     6601 aacgaccaca tgtttacccg cctcaagcgc cgctttggct aacgggaaat gggtatcgtt
     6661 gggtgtagga atgacaatca ggtctatgtt gggatcgtta aacagatgct tcggctcaga
     6721 gacaaccgta accgttggcc agtcggcttt tacttttgtt tcatcactgc tggagattac
     6781 cgccagttcc agcccgggcg tgcccgcaat caggggcgca tggaaggttt tgctcgcata
     6841 accataccca atcaacccaa cacggatgtt gtcgctcatg tcattgcctc tcattgaagt
     6901 atgatggcta tttgacacta tcctttaccc acgctcaaca gtttaataac ctgccagcaa
     6961 taagggatgt tgtttaactt aagtcaaaaa aatagcgaat tttccaacga caaaagctaa
     7021 atatcgcaaa aacctcagta aaaatcttgc tggagctatt attgctaagt aacatttacc
     7081 ccctgaagtt aatggatcaa tcaagagaga tgtgggctgt aatgaatcgt cttattgaat
     7141 taacaggttg gatcgttctt gtcgtttcag tcattcttct tggcgtggcg agtcacattg
     7201 acaactatca gccacctgaa cagagtgctt cggtacaaca caagtaagct ctgcacttgt
     7261 ggagcgacat gctgcccgtc cgggtgcatg ttttcacttg tcggatatta aaccaggaat
     7321 ttattatctt gttcgatgtt gttggtgatt gtcagggata gtaaagcgcg ttactctttt
     7381 cagagcagtt tttcatccct gaatatttgc catcagcgca ttcttattat tgtaataaca
     7441 caagcgttca cttttggtga aattttatca tcactaataa tacctacccc tttggtatta
     7501 attattttat ggacctttta tgactgttca ggactactta ttaaaatttc gcaaaatcag
     7561 ttcactcgaa agtctggaaa aactctacga ccatcttaat tacaccctga cggacgatca
     7621 ggaactgatc aatatgtatc gtgctgccga tcaccgtcgc gcagagctgg tttctggcgg
     7681 gcgtttgttt gacctcggcc aggtaccgaa gtccgtctgg cactatgtcc aataaagaaa
     7741 gtagcgatcg gttataaaag ccttataata acgcccctaa aattagattt gcctgtttgc
     7801 gtcgctattc tggagaacct atgaccacca caacgccaca acgtattgga ggctggttgc
     7861 ttggcccgtt ggcctggctt ttagtcgctc tgttgagtac gacgctggcg ttgttgctgt
     7921 ataccgctgc gttatcttct ccgcaaacat ttcaaacgct cggcggacaa gcactgacga
     7981 cacaaatctt atggggcgtt tcttttatta ccgctatcgc catgtggtat tacacactgt
     8041 ggttgaccat cgctttcttt aaacgtcggc gttgtgttcc taaacactat attatctggc
     8101 tgcttatttc cgtattgctg gcagtaaaag cattcgcttt ttcacccgtg gaagttggca
     8161 tagccgttcg ccagttgctg tttactttgt tggcaaccgc attgatcgtc ccctatttca
     8221 aacgctcgtc gcgggtaaaa gcgacgtttg tgaatccgta ataaccttac agttaacctg
     8281 ttgtcgcctg ctctggatta acggataata ggcggctttt ttatttcagg ccgaaaaatg
     8341 actgactacc tgttactgtt tgtcggaact gtactggtca ataactttgt actggtcaag
     8401 tttctcggtc tctgtccgtt tatgggggtt tccaaaaaac tggaaaccgc gatgggcatg
     8461 gggctggcaa caacgtttgt gatgacgctg acgtctattt gcgcctggct tatcgatacg
     8521 tggattttga tcccacttaa tctgatttac ctgcgcaccc tggcatttat tctggtgatt
     8581 gctgtggtcg tgcagttcac cgagatggtg gtgcgcaaaa ccagcccggt gctttaccgt
     8641 ctgctgggga tttttttgcc gcttatcacc accaactgtg cagtgctcgg cgtggcgttg
     8701 ctgaatatca atctcgggca caatttcttg cagtcggcgc tgtacggttt ttccgccgct
     8761 gtcggtttct cgctggtgat ggtgctcttc gccgccatcc gcgaacgcct tgctgtggct
     8821 gatgtcccgg caccttttcg cggtaatgcc attgcgttaa ttaccgcagg tcttatgtct
     8881 ctggccttta tgggctttag tggtttggtg aagttgtaat gaatgctatc tggattgccg
     8941 ttgccgccgt gagcctgctg ggcctggcgt ttggcgccat tctgggttat gcctcccgcc
     9001 gttttgcggt ggaagacgat ccggtcgttg agaaaattga cgaaatctta ccgcagagcc
     9061 agtgtggtca gtgcggttat cccggctgtc gcccctacgc ggaagccatc agctgtaacg
     9121 gtgaaaaaat caaccgttgc gccccaggtg gcgaagctgt gatgctaaaa attgccgagt
     9181 tgcttaatgt cgagccgcag ccgctggatg gcgaagcgca agagctaacg cctgcgcgga
     9241 tggtggcggt tattgatgaa aataactgta ttggctgcac taaatgtatt caggcttgtc
     9301 cggtagacgc catcgttggc gctacccgtg tcatgcatac ggtaatgagt gatctctgta
     9361 cgggctgcaa tttatgtgtt gatccgtgcc cgacgcactg catctcgttg caaccggtcg
     9421 cagaaacacc tgactcctgg aaatgggatc tgaacaccat tcccgtgcgt atcattcccg
     9481 tggaacacca tgcttaagtt attctctgca ttcagaaaaa ataaaatctg ggatttcaac
     9541 ggcggcatcc atccaccgga gatgaaaacc cagaccaacg gtacccccct gcgccaggta
     9601 ctactggcgc agcattttgt tattccactg aaacagcata ttggcgctga aggtgagttg
     9661 tgcgttagcg tcggcgataa agtattgcgc ggccagccgc ttacccgtgg tcgcggcaaa
     9721 atgctgcctg ttcacgcgcc cacctcgggt accgttacgg ctattgcgcc ccactctacg
     9781 gctcatcctt cagctttagc tgaattaagc gtgattattg atgccgatgg tgaagactgc
     9841 tggatcccgc gcgacggctg ggtcgattat cgctctcgca gtcgcgaaga gttaatcgag
     9901 cgcatacatc agtttggtgt tgccgggctg ggcggtgccg gattcccgac aggcgttaaa
     9961 ttgcagggtg gtggagataa gattgaaacg ttgattatca acgctgctga gtgcgagccg
    10021 tacattaccg ccgatgaccg tttgatgcag gattgcgcgg ctcaggtcgt agagggtatt
    10081 cgcattcttg cgcatattct gcagccacgc gaaattctta tcggcattga agataacaaa
    10141 ccgcaggcga tttccatgct gcgcgcggtg ctggcggact ctaacgatat ttctctgcgg
    10201 gtgattccaa ccaaatatcc ttctggcggt gctaaacaat taacctacat tctgaccggg
    10261 aagcaggttc cacatggcgg gcgttcatcc gatatcggcg tattaatgca aaacgtcggc
    10321 actgcttatg cagtgaaacg tgccgttatt gatggcgagc cgattaccga gcgtgttgta
    10381 accctgactg gcgaagcaat cgctcgcccg ggcaacgtct gggcacggct ggggacgcca
    10441 gtgcgtcatt tattgaatga tgccggattc tgcccctctg ccgatcaaat ggtgattatg
    10501 ggtggcccgc taatgggctt taccttgcca tggctggatg tcccggtcgt aaagattacc
    10561 aactgtctgt tggctccctc tgccaatgaa cttggcgaac cacaggaaga acaaagctgc
    10621 atccggtgta gcgcctgtgc tgacgcctgc cctgtggatc ttttgccgca acagttgtac
    10681 tggttcagca aaggtcagca acacgataaa gctaccacgc ataacattgc cgactgcatt
    10741 gaatgtgggg cttgcgcgtg ggtttgcccg agcaatattc ccttggtgca atatttccgt
    10801 caggaaaaag ctgaaattgc ggctattcgt caggaagaaa agcgcgccgc agaagccaaa
    10861 gcgcgtttcg aagcgcgcca ggctcgtctg gagcgcgaaa aagcgactcg ccttgaacga
    10921 cataagagcg cagccgttca acctgcagcc aaagataaag atgcgattgc tgccgctctg
    10981 gcgcgggtga aagagaaaca ggcccaggct acacagccta ttgtgattaa agcgggcgaa
    11041 cgcccggata acagtgcaat tattgcagaa cgggaagccc gtaaagcgca agccagagcg
    11101 aaacaggcag aactggagca aactaacgac gcagcaaccg ttgctgaacc acgtaaaact
    11161 gccgttgaag cagctatcgc ccgc
//
LOCUS       DAJTSI010000128        11110 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000128 DAJTSI010000000
VERSION     DAJTSI010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.128"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..459)
                     /gene="intS"
                     /locus_tag="OO327_003102"
     CDS             complement(<1..459)
                     /gene="intS"
                     /locus_tag="OO327_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCR6019549.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKSSSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(621..695)
                     /locus_tag="OO327_003103"
     tRNA            complement(621..695)
                     /locus_tag="OO327_003103"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Arg,seq:cct)
     gene            complement(769..1701)
                     /locus_tag="OO327_003104"
                     /pseudo
     CDS             complement(769..1701)
                     /locus_tag="OO327_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1995..2750
                     /gene="mlaA"
                     /locus_tag="OO327_003105"
     CDS             1995..2750
                     /gene="mlaA"
                     /locus_tag="OO327_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR6019550.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2932..3990)
                     /locus_tag="OO327_003106"
     CDS             complement(2932..3990)
                     /locus_tag="OO327_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019551.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTK
                     YQNEINQLSALGVNFDDI"
     gene            complement(4356..5696)
                     /gene="fadL"
                     /locus_tag="OO327_003107"
     CDS             complement(4356..5696)
                     /gene="fadL"
                     /locus_tag="OO327_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR6019552.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6068..6352
                     /locus_tag="OO327_003108"
     CDS             6068..6352
                     /locus_tag="OO327_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR6019553.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVKSEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            6532..7842
                     /gene="fadI"
                     /locus_tag="OO327_003109"
     CDS             6532..7842
                     /gene="fadI"
                     /locus_tag="OO327_003109"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR6019554.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWP
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7842..9986
                     /gene="fadJ"
                     /locus_tag="OO327_003110"
     CDS             7842..9986
                     /gene="fadJ"
                     /locus_tag="OO327_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR6019555.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGVVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            10189..10674
                     /gene="sixA"
                     /locus_tag="OO327_003111"
     CDS             10189..10674
                     /gene="sixA"
                     /locus_tag="OO327_003111"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR6019556.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2778 a         2542 c         2747 g         3043 t
ORIGIN      
        1 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
       61 aatctcaagg ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcagt
      121 cgcataccct actgaccaga cttgcttctt gtgttcgtac cattccttgt aaatcgcact
      181 aaatgaattg ttgttagacg aagacttttt cgcctttacc ggatcgatgc cagccgagat
      241 gtctttcctc gcagtccatg ctttatccct tgcctcctgc aaagtcataa gcggatattt
      301 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
      361 cccggataca gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
      421 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa
      481 aattatttta tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac
      541 catgacgaac tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg
      601 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
      661 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcgagtata cccgctaatg
      721 gactgcgggg taagtacgct gccgctcgat tgcttaaacc ctcgccattt atgctgggtt
      781 tttatcattt ttcttaatgt tttccgcacg ttctgctttt tggcgtgctt ctgctttacg
      841 cttgttgctc atgtcgttac gaatctgtgc atgactcatt aacgcgaaga taaaggtgcc
      901 gccgcagatg ttccccgcta aagtaggtag tgcgaagggc cagatgaaat cgctccagtg
      961 cagcgtaccg ttaaacacca gatagaggat ttcaacagaa ccgaccacga tatgggtggt
     1021 gtcacccagg gcaataagcc aggtcatcaa tataatcacc acaatctttg ccgcacctgc
     1081 tgcaggaaac atccatacca tagtggcgat cagccagccg gaaatgatcg cgttggcaaa
     1141 catctcgctg ggggtgttct tcatcacatc catgccgatt ttgacaaatg catcgcgagt
     1201 ttcttcattg aagataggca tatattcaaa tgcccacgca gcaataccag tcccgagaat
     1261 attacccagc agcacgactc cccataaccg cataagtaag ccaacgttgc tcattgtcgg
     1321 tttttgcatg acgggtagta ccgcagtcac ggtgttttcg gtaaataatt gctggcgggc
     1381 cattatgacg ataataaaac caaaggtata accgagattc tccagcaaga agctgcccgg
     1441 cacaccttcc agttcggcat gaaatatccc ttttgccagt aacgaagcgc ccatcgacag
     1501 acccgccgca atggctgacc acagtagcgc cattgcgtcg cgttctagct ctttttcacc
     1561 atcctggcgg atatgctcat gaattgccat cgctcgggag gggagtcggt cttcatctat
     1621 ttctattttt ttgccgcgct ctttttcttc gctctaaact tcaatttcgt cgctgtgttg
     1681 atcaattttg tcgttgtcca tggtctcttc tcttgaatta gcacgtatag ctaaagcgta
     1741 gcggcttttt tgctcgcaac tggcgggagt tactctgaaa atgtagaaaa ggctgcgttt
     1801 gcctttttct gtttctatag aatcaagtag cctacagggc ggcgattacc aggctatgat
     1861 caaatcagca aatcagggcg tctggacatc agttgacgtg ctgttacaat cgcccacacc
     1921 taaacaggcg gatacggtat cgttccgtca tggatggcaa actgcataag ccataaaaaa
     1981 acagggagac atttatgaag cttcgcctgt cggcgcttgc tctgggaact acgcttctgg
     2041 tggggtgtgc gagttccggt acagatcagc aagggcgttc tgacccgtta gaagggttca
     2101 accgcaccat gtacaacttc aacttcaacg tattagaccc gtatattgtt cgaccggtcg
     2161 ctgtcgcctg gcgtgattat gttccgcaac cggcgcgtaa tggtttgagc aactttactg
     2221 gcaaccttga agaacctgcg gtgatggtta actacttctt gcagggcgac ccttatcagg
     2281 ggatggtcca ctttacccgc tttttcctga acaccatttt ggggatgggc ggctttattg
     2341 atgttgcagg gatggcgaac ccgaaactgc aacggactga acctcaccgc ttcggtagta
     2401 cgcttggtca ttatggcgtg ggttatgggc cttacgttca gttaccgttc tacggtagct
     2461 tcacgctgcg tgatgacggt ggtgatatgg cggatgctct ttacccggtt ctttcctggc
     2521 tgacctggcc gatgtctgtg ggtaaatgga cgcttgaagg gatcgaaacc cgcgctcagc
     2581 tgctggattc cgatggtctg ctgcgtcagt cgtccgatcc ttatattatg gtgcgcgaag
     2641 cgtacttcca gcgtcatgat ttcatcgcta atggcggcga actcaaaccg caggaaaatc
     2701 cgaacgcgca agcgattcag gatgatttaa aagatatcga ttctgaataa gaaacaaata
     2761 aaaaaggtga gtcgcaaaac tcaccttttt tgttgttatc cctcaattac cacaagtaaa
     2821 aggagataaa ccgacaatga cccggacgta atgatgtaaa aatactgatg tttgtcactg
     2881 actatttccg atattcaggc gttgaaaaat caaaaaactg gaaaaataat tttaaatatc
     2941 atcaaaatta acacctagtg cagatagttg attaatttca ttttgatatt tcgtctggcg
     3001 attttgaagt cttttttcaa ctttatcttc gagattctta aagtctttgt atttattttc
     3061 aggatggtca acgtcaactt tgttagcgaa taaagtagaa attatttctt ttacattatc
     3121 gcgtctaaat tttgcatcgt ccacttgaaa attatatctt attgctcgac ctaaacgttt
     3181 aaaaacttcg ttaggttctc tcagtctttc cccgttgagc tcgtttacaa cctgatgatc
     3241 ggaaccagtt gcaaatgatt ctgccatgat agctttactg ctttcaaacc acgctgaagc
     3301 actcctggcg ttttcactac aatattgggt gagactatca ccattaatac gaattcctct
     3361 ttgaatgtgg tagtcgtcct gatcgtagta accgagagat aagtaatcat ttccgttttt
     3421 atttttaatt acaatagcga gagagtcgcc ttttttatct ttaccgtacc aggtaaataa
     3481 gtcagttggt tcgcttttcc cgctctcatc aaattccgtt accgtatgtc tgagtttgtg
     3541 tgatatctta tcgaagataa aaccaggatc tccatcatat atttctttta atgcgctgaa
     3601 gatcatttcc ttgttattga tatcatgagt cttctcaact atgttgtcat taatgatagt
     3661 acggacgagt tctttgtctt tatagttcgc aggtaattct attggttctt ggttgtcgat
     3721 accaatagta atatggtctg tatcattgga gccaatatca atataagtaa ctgaggaatt
     3781 attaatatta gatctataag gatcgtcgtt tactcgtatg gcgtttttta taagctcaag
     3841 tagatgttta atacaggtgc ttattccttc cttaccatta aaaaggtttt tgaactcttg
     3901 tactaatcca ttaatttcat cattgttatt ttcattgatg gattctgggt aagctgcgct
     3961 ggtattgttg attgaaatag atggtagcat gattttattc catgttgatt gatttgttac
     4021 aaatatatat tacctaatga aatagactat caaaaaacgc tcatttattt agaacctatc
     4081 tttaatttga gtgtataagc gcacaatagc caggggggtt gagtatcctg gaaatgcaga
     4141 aaagtaaact ctttgagaag gacgagcaga acgcttgaaa atatgcattt cccttcaggt
     4201 ttgtctgtgg ataccactta ctgatttgaa agggcaagct gccatcaggc tgtttgcaaa
     4261 actcaggagg caagcaatga ggaaactact ttcggtgaag tgggggagtt aaagtcacct
     4321 gcatagcagg tgactttatc caggcgaacg cgttatcaga acgcgtagtt aaagttagta
     4381 ccgaacagcc aggctttacc ttcagactcg aactggtatg ggccttcgtt aattttcacg
     4441 ctctgaccgt gcatataaga aacaccaacg tcgactgaag catctttatt aaatgcgtaa
     4501 gtcgtacctg cactcagcca gaaacggtcc tggtccggaa tggagataga acgattctga
     4561 gccggaactg ggctgtcatc aaaggcgata ccggtacgga aggtccagtt atcatcgtag
     4621 taataagtgg tacccaacgc gatgcggtaa gcatctttaa agccttcatg tttctggaac
     4681 agcgtgtcgc cactggttga ggtcgctttc agctgctgga actgactcca gctggtgtaa
     4741 gccaggctat agtgaatcgc ccactgtgga tcaacacggt tataacctga cacttcccac
     4801 atttcaggca ggttcagcgt cagataaccc gattgcgttg cgccacctgt cgcggtagga
     4861 attggcaaac cgtagttatt aaacgcacga ttaagatcgc tgctgtagtt acctttgaag
     4921 tcaattttca cttcagaacg gtaggtcagt gcatagcggt tatttttatc cagttcatac
     4981 aggattccgg cgttccagcc aaagccccac tggttaccgt tcagatgagc gattttggta
     5041 ttactgtcga taccgttggc ggtagctgcc aatgcttgcc cttgaggagt ttgcccggca
     5101 ggagattgca taatctgacc agcaaccaac tgccccagat cgcctgcgaa acgttcaatt
     5161 ttcgcgcgag cgtagatggc gttgaaacca agaccaaagc tccatgcatt atttaagcga
     5221 tacgcaccgc ttaagttcag gttcatggtt tcaaggtcag ttgtaccccc gacagagccg
     5281 cctgcataag tatcattaaa ctctgttgcc agaccatagt tagaggtaat agaagcgccc
     5341 caaccaaatt ggtcgttaat cggtgcaaca aagtgcatgt tcggaaccca tgccgtaggc
     5401 gcgatgttat cggctttcag gctacgacca gacggagacg ttccgctgat attcacatcc
     5461 gggtcaatat aaaccgcacc cgcagaaaat gtcgggcggt caaacatggt aatcaatgcg
     5521 gggttacggc taacgttacc tgcatcatcg gcaattgcgc cttcccctga ataagcccgg
     5581 cccaggccag aggaagaaaa ttcgtttaac tgaaagcctg ccgaccaggc ctgggtggag
     5641 ataagtgcca ctgcgactgc gagagcagac tttgtaaaca gggttttctg gctcatgacc
     5701 ataacctcaa tgatttattt ttatacaaac tatgttacgt gctgtaacag gagcgcgaag
     5761 tgtagggtct gaggtacatc ttagaaatca gaccagtggc gagagtatag gtcggaccag
     5821 ctagaatgtt gcaagtatgt ttccatcttt tttcaaatat gatctaagaa acgcgatcca
     5881 gctggcaaaa tttggaggtt gcgcactcgc ggattcgggt gaattttgtt ttagatcatt
     5941 tttaagtgtg atttcggtca cttatccgat tttgcagaat taacaactgg agcagcactt
     6001 tccggaagcg taaaatatcc acaacgttta tctggcacct tgggtgcaca tacagaggaa
     6061 atcaacgatg agtaaatgta gtgctgatga aaccccggtt tgctgctgta tggatgttgg
     6121 caccattatg gacaactccg actgcaccgc gtcttacagc cgcgtgtttg caaaccgcgc
     6181 agaagcagaa caaacgctgg cggcgctgac tgaaaaatcc cgtagcgtga aatccgaacc
     6241 gtgcaaaatc tccccgactt tcactgaaga gtctgacggt gtgcgcctgg atatcgattt
     6301 cacttttgcc tgcgaagctg aaatgctgat cttccagctg ggtctgcgtt aattcctctc
     6361 gggtacgtac gcccctgtca gttgctggca ggggcgtttt cttttctttc ttctgtcttc
     6421 actgtgtcat agctcccact ttcctttttc tccgcttggc taaatgtaaa aaaatggtta
     6481 agactatgat caggtcagac cactttattt atttttttac aggggagtgt tatgggtcag
     6541 gttttaccac tggttacccg ccagggcgat cgtatcgcca ttgttagcgg tttacgtacg
     6601 ccttttgccc gccaggcgac ggcttttcat ggcattccca cggttgattt agggaagatg
     6661 gtggtaggcg aactgctggc acgcagcgag atccccgctg aagtgattga acagctggtc
     6721 tttggtcagg tcgtacaaat gcctgaagcc cccaacattg cgcgtgaaat tgttctcggt
     6781 accggaatga atgtgcatac cgatgcttac agcgtcagcc gcgcttgcgc taccagtttc
     6841 caagcggttg caaacgtcgc agaaagcctg atggcgggaa ctatccgagc ggggattgcc
     6901 ggtggggcag attcctcttc cgtattgcca attggcgtca gtaaaaaact ggcgtacgtg
     6961 ctggttgatg tcaacaaagc tcgaaccatg agccagcgcc tgaaactctt ctctcgcctg
     7021 cgtttgcgcg acttaatgcc cgtaccgcct gcggtagcag aatattctac tggcttgcgg
     7081 atgggcgaca ccgccgaaca aatggcgaaa acctacggca tcacccgaga acagcaagat
     7141 gcattagcgc accgttcgca tcagcgtgcc gctcaggcat ggccagacgg aaaactcaaa
     7201 gaagaggtga tgactgcctt tatccctcct tataaacaac cgcttgtcga agacaacaat
     7261 attcgcggta attcctcgct tgccgattac gcaaagctgc gcccggcgtt tgatcgcaaa
     7321 cacggaacgg taacggcggc aaacagtacg ccgctgaccg atggcgcggc agcggtgatc
     7381 ctgatgactg aatcccgggc gaaagaatta gggctggtgc cgctggggta tctgcgcagc
     7441 tacgcattta ctgcgattga tgtctggcag gacatgttgc tcggtccagc ctggtcaaca
     7501 ccgctggcgc tggaacgtgc cggtttgacg atgagcgaac tgacattgat cgatatgcac
     7561 gaagcctttg cagctcagac actggcgaat attcagttgc tgggtagtga acgttttgct
     7621 cgtgaagtac tggggcgtgc acatgccact ggcgaagtgg acgatagcaa atttaacgtg
     7681 cttggcggtt cgattgctta cggacatccc ttcgcggcga ccggcgcgcg gatgattacc
     7741 cagacattgc atgaacttcg ccgtcgcggc ggtggatttg gtttagttac cgcctgtgct
     7801 gccggtgggc ttggcgcggc aatggttctg gaggcggaat aatggaaatg gcatcagcgt
     7861 ttactcttaa tgttcgtctg gacaacattg ctatcatcac catcgacgta ccggatgaga
     7921 aaatgaatac cctgaaggcg gaatttgcct cgcaggtgcg cgccattatt aagcaactcc
     7981 gtgaaaacaa agagttgcgc ggcgtggtgt ttatctccgc gaaaccggac aattttatcg
     8041 ccggagcaga catcaacatg attggcaact gcaaaacggc gcaagaagcg gaagcactgg
     8101 cgcggcaggg ccaacagttg atggcggaga ttcatgcttt gcccattccg gttatcgcgg
     8161 ctattcatgg cgcttgcctg ggcggtgggc tggagctggc gctggcgtgc cacggtcgcg
     8221 tttgtactga cgatcctaaa acggtgctcg gtttgcctga agtacaactt ggattgttac
     8281 ccggttcagg cggcacccag cgtttaccgc gtctgatagg cgtcagtaca gcattagaga
     8341 tgattctcac cggaaaacaa cttcgggcga aacaggcatt aaagctgggg ctggtggatg
     8401 acgttgttcc gcactccatt ttgctggaag ccgctgttga gctggcgaag aaggatcgcc
     8461 catcttcccg ccctctacct gtacgcgagc gtattctggc ggggccgtta ggtcgtgcgc
     8521 tgctgttcaa aatggtcggc aagaaaacag aacacaaaac tcaaggcaat tatccggcga
     8581 cagaacgcat cctggaggtt gttgaaacgg gattagcgca gggcaccagc agcggctatg
     8641 acgctgaagc tcgcgccttt ggcgaactgg cgatgacgcc gcaatcgcaa gctttgcgta
     8701 gtatcttttt tgccagtacg gacgtgaaga aagatcccgg tagtgatgca ccgcctgcgc
     8761 cattaaacag cgtgggaatt ttaggtggtg gcttgatggg cggcggtatt gcttatgtca
     8821 ctgcttgtaa agcggggctt ccggtcagaa ttaaagatat caacccgcag ggcataaatc
     8881 atgcgctgaa gtacagttgg gatcagctgg agggcaaagt tcgccgtcgt catctcaaag
     8941 ccagcgaacg tgacaaacag ctggcattaa tctccggaac gacggactat cgcggctttg
     9001 cccatcgcga tctgattatt gaagcggtgt ttgaaaatct cgaattaaaa caacagatgg
     9061 tggcggaagt ggagcaaaat tgcgccgctc ataccatttt tgcttcgaac acgtcatctt
     9121 taccgattgg tgatatcgcc gctcacgccg cgcgacctga gcaagttatt ggcctgcatt
     9181 tcttcagtcc ggtggaaaaa atgccgctgg tagagattat tcctcatgcg ggtacatcgg
     9241 cgcaaaccat cgccactacg gtaaaactgg cgaaaaaaca gggcaaaacg cccattgtcg
     9301 tgcgtgacaa agccgggttt tacgtcaatc gcatcttagc gccttacatt aatgaagcta
     9361 tccgcatgct gaccgaaggt gaacgggtag agcacattga tgccgcgcta gtgaaatttg
     9421 gttttccggt aggcccaatc caacttttgg atgaggtagg aatcgacacc gggactaaaa
     9481 ttattcctgt actggaagcc gcttatggag aacgttttag cgcgcctgca aatgttgttt
     9541 cttcaatttt gaacgacgat cgcaaaggca gaaaaaatgg tcggggtttc tatctttatg
     9601 gtcagaaagg gcgtaaaagc aaaaaacagg tcgatcctgc catttacccg ctcattggcg
     9661 cacaagggca ggggcgactc tccgcaccgc aggttgctga acggtgtgtg atgttgatgc
     9721 tgaatgaagc agtacgttgt gttgatgagc aggttatccg tagcgtgcgt gacggggata
     9781 ttggtgtggt atttggcatt ggttttccgc catttctcgg tggaccgttc cgctatatcg
     9841 attctctcgg cgcgggcgaa gtggttgcaa taatgcaacg actggccacg cagtatggtt
     9901 cccgttttac cccttgcgag cgtttggtcg agatgggcgc gcgtggggaa agtttttgga
     9961 aaacaactgc aactgacctg caataagaag gtcaaagcta tatgaatccg cgctgaatgg
    10021 cggagtgttg gtcaaaatgt aaacgcatat tgactatact tacgccattg aggtaaaaaa
    10081 cagcgtttca ttcggtgaat ggataaggca caatgccggc caccgttttc tttctctggt
    10141 ttcagatgaa agaaaacggg cgaatctggt taacaaaagc ggtgcaatat gcaagttttt
    10201 atcatgcgtc acggcgacgc agccctcgat tccgccagtg attcggttcg tcctctgacc
    10261 actaatggtt gtgacgaatc tcgcctgatg gcgaactggc tgaaaggtca aaaagtggaa
    10321 atcgaacgcg ttctggtgag cccgttcctg cgagccgagc aaacactgga agaagtaggg
    10381 gattgtctga acctgccttc cagtgcagaa gttctgccgg agttaacgcc ctgcggcgat
    10441 gttggtctgg tcagtgccta tttgcaggcg ttgaccaatg aaggtgttgc ttcggtactg
    10501 gtgatctctc acttaccgtt agtcgggtat ctggttgccg agttatgtcc gggcgaaacg
    10561 ccgcctatgt ttaccacctc ggccattgcc agcgtaacgc ttgatgaaag cggaaacggt
    10621 acattcaact ggcagatgag cccgtgtaat ctgaagatgg caaaagctat ctgatgtgtt
    10681 ccaaattatg cccaatgagg tagattcatt gggcataatt gctcattagg cagcggatat
    10741 ctgtcaacct cactactatc gaagactctc cttatctata aataaaacac tttctacatt
    10801 cattcggaaa ttattcttct acgcgtatct tgattttgcc caaagttaca tgttgaaatg
    10861 aataatatct gtttgacact cattgtcatt tattaataca gataatagag aaaatagagt
    10921 tgaataatca ggcaggatgt aattgaaact tgattaaatt ctggctatag tgtttatata
    10981 ttatattgtc aaaagtggat aaatgccaat cgcattatat gttcattgtt tgttttttaa
    11041 gctgttgata ttcattattg atatactttc tattttttct aaatacttat tccgtatttt
    11101 ttaaggatgt
//
LOCUS       DAJTSI010000129        11046 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000129 DAJTSI010000000
VERSION     DAJTSI010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11046
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.129"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            409..1239
                     /locus_tag="OO327_003112"
     CDS             409..1239
                     /locus_tag="OO327_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR6019557.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="OO327_003113"
     CDS             complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="OO327_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR6019558.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2368..3828)
                     /locus_tag="OO327_003114"
     CDS             complement(2368..3828)
                     /locus_tag="OO327_003114"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR6019559.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="OO327_003115"
     CDS             complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="OO327_003115"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR6019560.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYKVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     IR"
     gene            5433..6776
                     /gene="gntP"
                     /locus_tag="OO327_003116"
     CDS             5433..6776
                     /gene="gntP"
                     /locus_tag="OO327_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCR6019561.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="OO327_003117"
     CDS             complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="OO327_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCR6019562.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="OO327_003118"
     CDS             complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="OO327_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCR6019563.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="OO327_003119"
     CDS             complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="OO327_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCR6019564.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(9005..>11046)
                     /gene="fimD"
                     /locus_tag="OO327_003120"
     CDS             complement(9005..>11046)
                     /gene="fimD"
                     /locus_tag="OO327_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCR6019565.1"
                     /translation="IGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKW
                     QHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVI
                     HGIARGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFT
                     VPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLA
                     DRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQ
                     LVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTV
                     TQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRD
                     QMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLSY
                     SVQTGYAGGDDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHANG
                     VTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNTL
                     ADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQSS
                     GIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2685 a         2826 c         2682 g         2853 t
ORIGIN      
        1 acgtagatga aaatacttgt caccgctgct acttaattcc ggtgggaaat tatttatttc
       61 cgcataatca gctatgtatt atttgaaaca attagtttgt aaataatata aattgataac
      121 atatcaaata catcaaaaat accattcaca ggcattatta atatagacct caatatagca
      181 atatcaaatt ccaattagta ataagatatg cttttaaatt gcatgcaaat aattttaaat
      241 gagcgttttt tgatacttta gagtgtttcc taccctttaa tatcactgtg tagagttgat
      301 atcactacaa cagaaacatg catgtcattt gtttacttct ctttttacta cagaaaaatt
      361 tatctatttt tctaaacatc agttaaatac tcataaaagg taactattat gtctatgcca
      421 ttaagcaatg ccgctaataa ccaattcaat acaaataatc acttccttca tcatccaaaa
      481 gtcgacagtg agttaacacg tcgatatgaa tatgcacgcc tggatacgga aaatatctat
      541 cttctgcctc ttgctcgtgg caataaccat aattacgatg gaaaaagcgt tgtcgaaatt
      601 agaaaactta atattggcaa acagtcctgg ccatttaatt atgtgacaga tacatgcaga
      661 gaattcaatg gtattacaac aacaggacgt atgctctatc gaaacttgaa aatcacctca
      721 gctttagacg aaatatatgg tggtatatgt aaaaaggcgc atgctgctac agagttagca
      781 gaaggtttac ggctaaattt atttatgaag agccctttcg atcctgttga ggactataca
      841 gttcatgaaa taacattagg ccctggttgc aatgttcctg gttatgccgg aaccacgata
      901 ggatatattt caacccttcc agcttctcag gcaaaaagat ggaccaatga gcagccaaga
      961 attgatatct atattgatca gataataact gtctcagggg tagcaaattc ttccggattc
     1021 gcccttgccg cattacttaa tgctaatatt gagatgggaa atgacccaat cattggtata
     1081 gaagcctatc ctggtaccgc ggagatccat tcaaaaatgg gatataacgt tatccccggt
     1141 gatgaagatg cgccactcaa acgcatgact ctgcaaccgt catctcttcc agaactgttt
     1201 gaattaaaaa atggtgaatg gaattatatt ggtaaatagc cactgagaag aatgagtact
     1261 aatattcagg ttatacagat atttaaacaa aaccctgcaa tttacaaagt aatactcatt
     1321 gcgacttctt aatttaagaa agcgcaagga acgtttaccc ttgcgcttat tataataagt
     1381 caggcgtcga ctttatccag cggccatgaa tcaaagagat agccgtcaaa ataaatatcg
     1441 tcaacgttcg agaattccag cagacgttgc ttaacgtttt ccagatgctg ccacattgcc
     1501 agcttcgccg ctcgggcatc ttttttgatt aacgcggcga ggatctgttt gtgatcgccc
     1561 aaccactctt tgcgatacag gctgtcatcc agatggctgt gcaactggat ccacattgga
     1621 ttgttttccc gccactgcca ggactgacgg aataactcca ccagcatgct gttatgcgtt
     1681 gcttcagcaa tggcgagatg gaactgcatg tcaccgcttt cgctgctacc cggcgcgctg
     1741 gaagccagtt cacgctcttc cagttgcaat gcctgacgca ttttgacgat atcttcgcgg
     1801 gtagcctgca gtgcggcaaa ctcggcgatg ttactttcca gtaactgacg cgcctgtaac
     1861 agctcaaaag gacccgcatc attgcagacg ttggcatccg gactgtctgt gttgtggctg
     1921 cctgagctat caagcacata gataccggca ccccggcgca cttccaccag ccctttgatc
     1981 tccagcatga tcagcgcttc acgtaccacc gtccgcgtga catcaagcat ttctgcaatt
     2041 tcacgctccg gcggcagccg ttcgccagga ttgtacggcg tctttatgat cagatcgtgg
     2101 atcatcgccc cgacttcctg gtaaggtctt tgcgcagagg tgacagattt catggtgttg
     2161 tgtccgttag gtgcggttaa tcaaattttt gccggatgcg gcgtgaacgc cttatccggc
     2221 ctacaacggg caattttacg gcccccgtta atctcaaact ataccattag cactggaagt
     2281 tggcacgtac aggtgggcaa aaaaacgaaa actggtcaac caaatatcag tttgattgac
     2341 cagtaagtct gtagggtaat cagttagtta cagcgcagcc acacactcgc gcgcaccgcg
     2401 ttcgcacagc tgctgatatg ccgacgtcac cgcgccaaca aagtcggcat tctgcggcag
     2461 atcatcggca aaaataccgc tcaggcccag cagcgctttc acgcggtctg cgccctgata
     2521 ctgcgcgtta atcttctgga actcggccag catcgggtcg accacgtcaa tggcattacc
     2581 ctgctcatcc acgccctggg tgtaacccat ccagccagcc acgcccagcg ccaggtgacg
     2641 ccagctgccg ccgttttgca ggtgcagacg caccgggtcc agtagacgct gcggtaactt
     2701 ctggctgccg tccatcgcaa tctgccaggt acggtgacgc agagacgggt tgctgaaacg
     2761 ctcgatcagc agcgtcgcat aggcgttcag gtccgtacct tccggcatcg acagcgttgg
     2821 cgcttgttcc tgcatcatca gggcaaaggc cgctttgcga taatccgggt tagtcatggt
     2881 gtcggcaatg gtttcatagc cgccgaggta accgaggtac gccaggaaag agtggctgcc
     2941 gttcagcata cgcagcttca tcatttcgaa cggcacaacg tctgcaacga actgtgtgcc
     3001 cactttatcc cagtccgggc gaccattaac gaagttatct tcaatcaccc actgacggaa
     3061 cggttcgcag gcaatggcgc acgggtcgta aacacccagc tggtcagcaa tttcctgtaa
     3121 ggtttctggc gtcgccgccg gaacgatgcg gtcaaccatg gtgcacggga aggtgacgtt
     3181 ctcttcaatc catgccgcta gctgcgggtc acgcgcctga gccagcccca gtaccgcgac
     3241 cttcgccaca tggccgtttt cacgcacgtt atcgcaggac atcaccgtaa atgctttcag
     3301 ccctttttca cgacgcagac gcaaggcttc gacgatgtaa ccaatcgcgg acttcggcgc
     3361 agtcgggttt tccagatcgt gcttgatcag cgggttattg agatccagtt gaccgcttgc
     3421 cgcatcagcg cagtagcctt tttccgtgac cgttagagag acaatcgccg tttgcggacg
     3481 cgccatcgcg ttgagaatac cttcgcagcc gtcgatttcc ggatgcagcg cttctttcat
     3541 cgaaccgata attttcagct cggtgctttc tgcgcctttt tccgctacgg tgtacagcag
     3601 ttgctgtttt ttcaggtttt cgatcagcat gcggtcgttg cctggcatca ggttaacttc
     3661 gcagatgccc cagtcgctgt cggtgctttc cagcagatga tgggtataca gcgcctggtg
     3721 cgcgcggtga aacgccccgc aaccgagatg cacaatgcgt gattccagac gagaatgatc
     3781 ccatgacggg cgggcaaccg gcagattgct gtcaacaata gtagtcatcg taaactccag
     3841 aatccgtctt cagagagtga cggcaaggaa gaaggaaccg gcacggcgcg cagccatgcc
     3901 ggtgggtatt aacggataaa gaaagcgcgc tggatcgcca gttcgacacc gcgaacttcg
     3961 gccaggcctt tcagacgacc aattgcggag taaccagggt tggttttctt cttcaggtcg
     4021 tccagcatct gatgaccgtg gtccggacgc atcgggatca ggtcttcttt gccttccgct
     4081 ttacgacggt gttcttcttc aacaatcgct ttcaccactt tgtacatatc aacgtcaccg
     4141 ttcaggtgcg ccgcttcgtg gaaggttttc gggttatctt cacgcatggt ggagcgcaga
     4201 tgggtgaagt aaatacgcgg accgaactgc ttgatcatat caaccagatc gttgtcagca
     4261 cgcacgccgt aggaaccggt gcacatggtg aaaccgttcg ccatgctgtt tacggtatca
     4321 accatccact gcatatcttc aatggtggaa acaatgcgcg gcaagccgag gatcgggcgc
     4381 ggcggatcgt ccgggtgaac cgccatacgc acgccaactt cttcagcaac tggaataatc
     4441 gctttcagga agacggcaaa gttttcgcgc agtttggctt tgtcgatatc tttgtacagc
     4501 tccaggtgct tacggaactg gtcgagggta tacccttctt ccgcgcccgg cagaccggca
     4561 atgatgttac gcgtcagacg cgctttgtct tcgtcgctca tagtggcgaa gcgttcagct
     4621 gcctgagcaa tttcttcttc ggtgtaatcc gcttccgcgc ctggacgttt caggatatgc
     4681 atttcgaatg cagcgaattc gatctggtcg aagcgcagag ctttggagcc gtctggcagc
     4741 acgtattcga ggtcagtacg ggtccagtcg agcaccggca tgaagttgta gcacacggtg
     4801 cgaatgccac actgcgccag gttgcgcaga gtctgctgat agttagcgat ccactgctca
     4861 tagttgccag tgtgggtttt gatatcttcg tgaattggta cgctttctac gacagaccac
     4921 accaggcctg cgtcttcaac gatcgccttg cgtttgagga tctcttctac ggaccatact
     4981 tcgccgttcg ggatatggtg cagcgcggta accacgccag ttgcgcccgc ctgacggaca
     5041 tcagctaaag aaaccggatc gtttgggccg taccagcgcc aggtctgttc catgattttt
     5101 cctctttcac atagtgggtt ccacagcaaa gagacgcgga accttggagt tgtttaggta
     5161 tccggatgaa caaccagacc gcagccgtgg cgttgcatcg gatgagatgg cgtataagtt
     5221 ctaccttttg gtaagacatg tcaaccagaa ttctgaaatt ggttaaccgc gtcgcaagaa
     5281 tttcactctc atggttgttg ctgcaaattg cagcaaaggg taatcgatgg ttattgatca
     5341 gaggatgtga cattcatcgc aacaatggtt gaccaattta cataacatgt cgcccaaaat
     5401 aacaacggtt caaccaagta aggtaaaacg ttatgcatgt gcttaacatt ctctgggtgg
     5461 tattcggcat tggcctgatg ctgatactga atttgaagtt caaaatcaat tcaatggtgg
     5521 ctttgttggt ggcggcgctg tccgtcggga tgctggcagg catggatttg atgtcgctgc
     5581 tgcacaccat gaaagcgggc ttcggcaaca cgctgggaga actggctatc atcgtggtgt
     5641 tcggtgcggt catcggtaaa ttgatggtcg actccggcgc ggctcaccag atagcacata
     5701 cgctgctggc gcgtctcggt ctgcgctatg tacagctgtc ggtgattatc atcggcctta
     5761 ttttcggtct ggcgatgttc tatgaagtgg cctttatcat gttagcgccg ctggttattg
     5821 ttattgccgc cgaagctaaa attccgttcc tgaaactggc gatcccggca gtagcagctg
     5881 ccactaccgc acattcactg ttcccaccgc agccgggtcc ggtggcgctg gtgaatgctt
     5941 atggcgcgga tatggggatg gtttatatct atggcgtact ggtgacgatc ccaagtgtaa
     6001 tctgcgcagg tctgatcctg ccgaagttcc tcggcaatct tgagcgccca acgccatcat
     6061 tcctgaaagc agatcaaccg gtagatatga ataatctgcc ctctttcggc gtttcgattc
     6121 tggtgccgct gatcccggcg atcattatga tctctaccac catcgccaat atctggctgg
     6181 taaaagatac ccctgcctgg gaagtggtta actttattgg ttcctcgccg attgcgatgt
     6241 ttattgcgat ggtggttgca ttcgtactct ttggcaccgc gcgtggtcat gacatgcagt
     6301 gggtgatgaa cgcttttgaa agcgcggtga agagtattgc aatggtgatt ctgatcatcg
     6361 gtgcgggtgg cgtgctgaag cagaccatca tcgacaccgg cattggcgac accatcggca
     6421 tgttgatgtc ccacggcaat atctcgccct acattatggc atggctgatc actgtgctaa
     6481 ttcgtctggc gacgggtcag ggtgtcgttt cggcgatgac cgccgccggg attatcagtg
     6541 ctgcaatcct tgatccagca accggtcagc tggttggcgt gaatccggcg ctgctggtac
     6601 tggcgacggc tgcgggttcc aacaccctca cccacattaa tgatgcatct ttctggttgt
     6661 tcaaaggtta ctttgacctg tcagtaaaag acacgttgaa aacctggggt ctgctggagc
     6721 tggtcaactc cgtggttggg ctgattattg tgctgattat tagcatggta gcgtaaaaga
     6781 acaccagagc ctgccaatgg caggctcaga ctgatgacta aagtctaaaa atgtgcccgg
     6841 acagtcccct cgcccctccg gggagagggt tagggtgagg ggaacagacc agtaaagtgc
     6901 gctttgttta tgccagatgc gacgctgacg cgtcttatct ggcctacaaa gggctaacgt
     6961 gcaggttttg taggtctgat agcgtagcgc ctcaggtacc agcattagca atgcgctgta
     7021 atttttttat tgataaacaa aagtcacgcc aataatcgat tgcacattcc ctgcagtcac
     7081 ctgccctccg gtacgtgcgt aatttgccgt taatcccaga cttaccgccg aagttcctac
     7141 tgctcctaac gataccgtgt tattcgctgg aataatcgta ccgttgcgcg tcaactgtac
     7201 gccgacgccc tgcgctggtg aaaacgacgc ggtattggtg aaaatcgagt tgcccgcatc
     7261 tgcggttgtg ccggagaggt aataccccag gttttggctt ttcgcacaat aaacggtaag
     7321 aggaattggc actgaaccag ggtagtccgg cagagtaacg gtgacatcac gagcagaaac
     7381 atcacaaccg ccagtgggca ccaccacatc attattggcg taaatattcc acacaaactg
     7441 aaaatcatcg ctgttatagt tgttggtctg tcgcaaaata agcacggcaa ttaatgaacc
     7501 agctttaata gccaccccgc ccgcactgct cacaggcgtc aaataaagcg ccaccggcca
     7561 cggcttatcc gttctcgaat tataaacaac ccgcggcgtt tcgctggtgg tcggaaatgg
     7621 atagctactg ccactatatt ttacggtccc ggaaaaatta gataacacgc cgccgtaagc
     7681 cgagcctcgt tgcagtgtga catagtctgt aatggtttcc ggataatcgt tatggcaaaa
     7741 gatttgcgtc gaaagatcta cgaccaggtt ttgccccaca ttcacgacag gcgcaaggtt
     7801 tacataaaca ttagcgctgc caccgccaat agggatagcg gtaccattgg cggttttaca
     7861 ggcgaatgac caggcattta ccgaccagcc catcagcagt acagcaaaca gggtaatagc
     7921 tcgtttcatt acaattatct cttcgggttc agctgtaggt ataggtgatg ctaatcactg
     7981 cctgaatggt tccctgagtg gctccgccat ttaccgtcaa tgctctgacc tgtaacggga
     8041 agtgcgctga ttgtgaggaa tcatccacct gaactgtttt ggttgcgcca gtattcaatg
     8101 tgttgccact gtcatcctgt agctctaact ggatgttttg cgcggtcccc tggtttttat
     8161 aatatccggt gctgtcggct gccccgctga agctggcagt gaccctcgac gttcccaccg
     8221 gacaattagt caactcaagc gcaacatcat gccaggccga tgccgccccg gcagacataa
     8281 gactgaaaga ataaagatcg ccgagatcta ccgtggcatt ggtggtggaa accgtacacg
     8341 gtttggcgac gaccttaccg ttcaccgtga tggtgacatc ggctgcctgt atcgtcgcac
     8401 ttgcgagcgc caacattgcc gccaatacat acccacgttt gcaccatttc atgagcatct
     8461 ccagttactg atattcaaga gtgaaggtag ccgtagcatt gatatgcccc gcagtgacag
     8521 gcacctgtgt cgccattagc cgggcgtaaa aattcagcgt atttggttta cccggcgtca
     8581 gggtcgtcca cgaaatcgca gacgatggag cattaagggg tatttgattt tgctgctcat
     8641 tcagaagctg tattcccagt cccgcagccg ctgacaccgt attttcaagt gcaagcaggt
     8701 tggcattgtg gctatctgca acgccggtaa acccaacttt tacggcagaa acggcattac
     8761 cacagggtga cagcaaaata cgaaatggaa cgacaggagt cgtcgcgcca atgttgttaa
     8821 attgcttcgc cgcgttttcc atcagatcaa cagtaaaatt ggttgattca gcggccacac
     8881 tacagccgtt atctctgaca tagccgcgga tagtaatcgt gctatccgca gccaaaacgc
     8941 gacttaccgc cagccacaaa aaagcgcaca gaagataaaa aggtttgttt ctcatcacgc
     9001 ccccttaacg acattcagct gatagctggg ttaataactg ctgctgactc tctggtggca
     9061 gttgataatt ggcgacacag tgagcatttt cctcttctcc ccatttcacc tgaacttttc
     9121 ccgctaaagg cattccgctg aggtaaacct gaccattatc cgcaacaatg ccgctactct
     9181 ggctactctc tgatgtcacc atcgccccaa acggcagcgg cttattattg tgagttagcg
     9241 tcatgagcag ttttatccca acgcgcgctt taaactctgc tcgcacgatc gccccacgag
     9301 tgggaacaac gttagcgacc gcgttatcta aatcgacgtt atcagccaag gtattggtat
     9361 ccagcgccac tctattttcc cgatattcag tggcataagg cagcacggca taaccacgcc
     9421 agtcggtacg cacccccgtc tggttttcga cttttgcatc ttttgcgcca ggcgctttaa
     9481 caagcaccac cgtatcgttt aacggctgcc ccagcgttac gccattggca tgagccaata
     9541 ccccaccgct gactccgtaa tagagctgct taatatcatc gctatggctg taaccgatat
     9601 tggcattgcc gtaaccaccg cgataattca gcgtggcgta gcctgtactt ccgctattac
     9661 catcgtctcc cccggcatag ccggtttgca cgctataact gaggttgttg tcttccagca
     9721 acgtaccgta tacaccagcc agattggtca tccgaccgtt gagatcgtgt gacatgctgt
     9781 agctggcact ggcatgtcgc cactgagatt tactgtcaga acgcagccag tggctgaaag
     9841 gaatattgac gttaagcgct aacatctgat cccgtccttt ttgccaggcg tttttcgtca
     9901 ggctatagct gagcgtccag ttgatatctt cgaacgcagt atttaatcca gcctggaatt
     9961 gctcatcgac attactcgtt ccccaataag tttgatggct accactcaaa tacagtgttg
    10021 atgtgcgccc gagttgctga gtaacggtga gctgtaattt cccgcgtttg ttataagcga
    10081 ggttgtaata gtcggtgaat ttcggcttaa cctgaataac tccatcctgt gtttcgatgt
    10141 tgtagccatt cattcgactg tatgttgtat cagcgaaatt aaaatatccg ctggtcgaat
    10201 aacggtaacc cactaactga atattcgtgc ctgattcatt gagcgatttg ttatagagaa
    10261 aacgcaccga ttgtccgtca tgctgactgt catcgggaag tgtggaatta gcctgcgtca
    10321 tatccacaga cagagcgccc agtgcctcca tgtttttccc gataccgaaa ttaaaagcac
    10381 gataacgatc cgccagttgc gttccaccat atattgtcca gccagccgga aggccgtgga
    10441 gtaatgtact ctggaaaaag cggggttttt cctgctgcgc atttccacta cggtattctc
    10501 ctgccgtaat ggaataacga gtatgccctt cacgttgcaa aagcgggact gacgaatagg
    10561 gtacggtaaa aatctgcgtg ctgccgtcag cctctttgat cgttacctgc aagtcaccac
    10621 tattacctgc ggcatagata tcgttgatgg taaaaggccc cggtggcacc gtactattat
    10681 aaatgccata tccattttgt ttaatagtga cctgtgcagt accacgagca ataccgtgga
    10741 tcaccggggc aaatcctctt tggctatcgg gtaacatatt gtcatctgag gccaattgtg
    10801 cgccgcgaaa gttaatacca tcgaaaatat cgccctgggt ataaccatca cccaacgtca
    10861 gccgggaacg taaaggaatt atgtctcgct caatccaggt aatgatatgc tgccatttat
    10921 ttttgctacc tgatgaactg tcgctactgt tataactcca ggtggtattg tcgcgtaaac
    10981 gccacgcacc aatatttaac ccactctgta ggtttaaata tgcataatgg ctgttacccc
    11041 caatcc
//
LOCUS       DAJTSI010000130        10890 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000130 DAJTSI010000000
VERSION     DAJTSI010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10890
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.130"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..270)
                     /locus_tag="OO327_003121"
     CDS             complement(<1..270)
                     /locus_tag="OO327_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR6019566.1"
                     /translation="MYRAAATHPKIKLLQFKLIPCPGMNKAIPSLIADFTGELHRLIS
                     EHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLMVLLLNNDVWC"
     gene            complement(364..926)
                     /locus_tag="OO327_003122"
                     /pseudo
     CDS             complement(364..926)
                     /locus_tag="OO327_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1130..1336)
                     /locus_tag="OO327_003123"
     CDS             complement(1130..1336)
                     /locus_tag="OO327_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCR6019567.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1443..2006)
                     /locus_tag="OO327_003124"
     CDS             complement(1443..2006)
                     /locus_tag="OO327_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR6019568.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3381..5216)
                     /locus_tag="OO327_003125"
     CDS             complement(3381..5216)
                     /locus_tag="OO327_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019569.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5317..5604
                     /locus_tag="OO327_003126"
     CDS             5317..5604
                     /locus_tag="OO327_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR6019570.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5576..7105
                     /locus_tag="OO327_003127"
     CDS             5576..7105
                     /locus_tag="OO327_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCR6019571.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7275..8492)
                     /locus_tag="OO327_003128"
     CDS             complement(7275..8492)
                     /locus_tag="OO327_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6019572.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8859..9845)
                     /locus_tag="OO327_003129"
     CDS             complement(8859..9845)
                     /locus_tag="OO327_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019573.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(9842..10370)
                     /locus_tag="OO327_003130"
                     /pseudo
     CDS             complement(9842..10370)
                     /locus_tag="OO327_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
     gene            10437..10890
                     /locus_tag="OO327_003131"
                     /pseudo
     CDS             10437..10890
                     /locus_tag="OO327_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3379 a         2507 c         2082 g         2922 t
ORIGIN      
        1 acaccagaca tcgttattaa gtagcagcac cataagataa ggagtggcat cgttagccac
       61 agcttcccta ctccagaggt aatataaagg ggtgggctcg actgatttat ctttacgtcg
      121 cttacactgc aaatgttcag aaattagtct atgcagctca ccagtaaaat cagcaatcag
      181 agaaggaatt gccttattca taccagggca aggtattaat ttaaactgta ataatttaat
      241 tttaggatgt gtagcagcag cccgatacag agttgcaagg acacactttt gccagagggc
      301 gttacaggaa agcttaatgt ttgattctgt atacataata aatcacctta tagttacaat
      361 aggtcaaaaa ccgctgtagc caaagttacg ctggcctgat gctttagtgt cgagcttcgt
      421 caaataatcc agacgtacca ataagcgctg gtactgctct gggaaattag gatcgttaat
      481 atccaggatg taacgtccat tagcagggaa atgaactaat ccctggtgat cttccagttc
      541 caaccctaag gcactgtacc aggctccggt aattaaccct ctcagggtgt cagagcgctc
      601 atagtttccc agatggtaat aggcgtcttt gttaaaaaaa ggcagacgtg ataatgacat
      661 ttaaaggact gcgtatattc tttcgcccag ataatacaaa ccgacgttgc gtaggtgcgt
      721 ttgccttgtt gttttttacg gtgttgatct gcattaattt tcgcattcag cgagttacgg
      781 aaacgagata tctctccggg gatcaggtta gggaaacagg taatgatatc cccgttgtca
      841 atcacaggag ggtaatggag atccccccgt attgctgtta ttctggaagt gtttttaata
      901 ttcgtattga tagttctcag agatatatca cactctagat attttaatta agtatcagtt
      961 cgatatcggc atattgtaca ctacctcagg tagatgatga gatcagatac agggatcaac
     1021 tgacggtgag cgcagtcccc acattttatc cagggcttac tgcatattcc caacagccat
     1081 tcgttagcca ggtagacgaa taccctgatt tgccgctggt tttgctttcc tatcgaaggg
     1141 cccatacatc atcacgccca cgaaacaacc gacgaaataa cgcaactttc tcatccgtgg
     1201 ataatacgaa aacgcactgc acagggctct gcggtttacg tcgccattca atcccatgag
     1261 cttcaagtaa tgagaccagc cgggtatttt ctgcccgcag tactgctagt tcatcgctgt
     1321 cagatttata aaccatatgc gttatcactt gtacctgtct cccggcggca ccacgactga
     1381 aattcgtgtg aaggcaaatc cacgcgataa cttacgatag ttcggacata agcccccata
     1441 attcagttat gtcgtggtcg accccggtta cttttttcgg gaagcctgct acggcccaga
     1501 ataataccgt gttctttctg atcctgttta gccagatagc taagggcgta acgtaagtcg
     1561 tctaccgcag atttatcact atagtgaatc acgtgatcga tacgcaccgg gtatttgtct
     1621 ttagcccggc acagatgaaa ataaccctcc ccttccgtga tccgcctcca gatatctccc
     1681 agttgcctgg atatctgata agagttcttg tgacgttgtc catccaggta gccgataaaa
     1741 tgaatatgaa gccctttgtc tggtgtatat tccattaccc agtaatatcc cgccaacatc
     1801 gtctgggttt cgctaagtaa acggtatatt tccatacaca tactgtgctt acaggaatgc
     1861 ccgaaactgg gcgtgtcttt cctgtaagca aaatcaattc tgaaaggtaa cagtttagaa
     1921 aaacgttgaa acataccatc catgtgttca ttcacgtctt tcagaatcat gaaatccatt
     1981 ttgtaattgg gattggcatt atacatttta tagtccttac tttataaaac tcacaggaag
     2041 agagaattac gtaaatactg agagtagaga aacgccgtac cacggcatta ataaacgaag
     2101 taaaccagca gcacagacaa cctgataaaa ctgttttgta ttaccgcatt actgtgatga
     2161 ggtaatactt cagaatacag tgagactctc ttaaactgag aaccagtacg aattacaatg
     2221 gaaaaacaca cctcatagaa ttgagtttca ggaagcacca ctaaattctt tacaggaagt
     2281 aatctgacat tcacaaaact cagtatcggc tagctacagg caacatcgct attaatattg
     2341 tcgaaccatt aagtaaagcc gagagagacc acgggtattt aatcattagc ataaaagaat
     2401 aatggtatac agaacatacc cacacaccat aagaagattg ttccatacag aaaagatcat
     2461 atttacctat acctataaac tgtaacggag tcataacacg ctctaatagt gttccgatac
     2521 gtaaccagtg ttaagtacgt acaggacaac attacacaca taattcacca tacagcttcc
     2581 cccatatgct tcactgcgta gtgtatggag atatatgcat tacacacata cagaatactt
     2641 agcataaaga ggagtgacat taccacgtaa agagataagt tatacaacaa acatacaaac
     2701 aatacggcac agacacacaa taccataact ccaactaata atactaatta accttaatta
     2761 tttatggtat atgaaataaa tgtgattctc ccggaactaa ctaacctaac cagacagatg
     2821 atcactgtat aaaaacttca gttaatactc atttgttttg tcgactcagt atataatgag
     2881 tgaccagtaa tcaggataat cacacttaca taacctgtaa aaaagaacaa gaagaataag
     2941 ccactaacat aatgcaaaat ggcatctgaa tcgatcgcag tttggcattc gaatcgatca
     3001 tgatttggcc caatccgtga tcgaatagag cggaatacgc aacataattc accagacaag
     3061 agcttacacc gaattacaga ttatgtatta atgtcaaaac tggcatattt tacagataca
     3121 atgaacatgc aacccatcaa tctaataggg gcggtatgta catctgccca tgaatgggat
     3181 ggcgctaaga atacaaaaca attagacata gatcacaaaa acacaccatt ctaattgaaa
     3241 aacaaaacaa acaaataaat catactatta tagaattttc tcttgccaga catagataat
     3301 acccctgcta attttgctaa tggttagcaa aattagcagg gtatataaag attattgacc
     3361 caaaacacac tcagatgaat tcacgacagc tcaggagggt tattcctgat gtcatcttca
     3421 caaataatgg ttaacgcatc atatatcatt ctgcgtctgt tcctgttttc ggccttatcg
     3481 ccatacaaca tcatcacatt tttaatttca tcgaccggtg atttcacata gtgaactaat
     3541 acaccaagct tctcaacaca ttgcagtaaa gtatatttct tactgcctgt gaaaagccct
     3601 atcatagttt ctttacgttc gcgactggat aatactttgt tttccataat gacttctgcc
     3661 agcgtcttac catcataaac atactcttta tctatcagaa tatttatctt atccagcacc
     3721 ttctctacgt catcaagcaa acctgtaata tatgggggcg caatatatgg tttcgtactg
     3781 atcttttcaa gcatacaatt atacatctta ataacacgca tcagggtaaa attgttcgca
     3841 tcatactgta tttcaccctc cttatataca ggatcaataa gtggtatttt ttcaataaat
     3901 gcatcctgat aggcttttac atggtcaagc caataaaaca acgagccata ctttatattt
     3961 tttctattta ctttaacctc cagtgctgtc cgaagcaacg ataatgcccc cctgacatat
     4021 cctgaaggta attcaccaaa caatcctttt ctttccatgt catcataatg ctgcagcaca
     4081 tcattaattt tatttttcct tatcagacaa atgtacaccg caagtgtttc gtagcagctg
     4141 gtcacatgaa cagcctcatt ctgcaactgc ccaaaatagc ttataatctt tttcagatat
     4201 gcgtcatcac aaccattctt atcattaata ttaaatacaa gatctttcag acgggaaata
     4261 tattcctgag atttatcatc aagtgaaaca ctataacgag acaatgttcc ttctgcactg
     4321 ttttgaaact tataaaacat ttccagtaat ttttttgcat aaggcgttgt atcaaagctg
     4381 gtaaagtcat aaagatattg cgcagcgtaa ccatacaaac catgaatcca gagtccttct
     4441 ctcaccaaca ctctgagata atatgcaagg ctttgaagta tcttcgtctt tcgttgtata
     4501 tcaagtaaga cacttaatga ataaaatgca ttatagaatt tatcaatatt cctgagcact
     4561 ttctccagtt catccttatc cagcttcttc tcccggtaat cctttttata ttcatttttg
     4621 atagccatga tatattgttg catcaggctc ttgcttttcc cgttatcctt ccgtatagtt
     4681 cctgtatatt tccccagagc atacagcttt ccattttcat gaaaatcttc cttgttgaat
     4741 cccagttcag ccagatattc ccgaagataa cgtttaacag cagcttcatc gctttctgta
     4801 tcagcgtgat caagacaagg acaatacgat ttctgtaaaa aattataaat atgataataa
     4861 aaagcagagt cattctctgg tctgtatgta ttatacatat ctacaacaag aaaaataacc
     4921 ccaaaagtgc gcagacaata gctgcgaagg aattcaggtt ttagtgtgga atgcactgga
     4981 taagaattca tcatgtaata aaaatgttca tatcgggcat atatgatccg ccgttgctcc
     5041 tgactgaaca ccccttcagc atcaataata cgactgatct cattataacg ttgatcccag
     5101 aatttttgat gcatttcaaa attggtactc ttccgcttgt gtgccggatt ctcgccttcc
     5161 ttaaacaacc ccaaaacagt acaaatatca tacaacatat ttgtaaatgt gcgcatatgc
     5221 tgtgctctcc ggttggtttg cattttatcg cagaggacat tctgagctta acgtatcaaa
     5281 ctgacacaac gacatgcaat gcatatggag aacaatatgt caaatcaaac cggacagctt
     5341 tacacgcctt cacgcatcct gcgtatggct gaacttaccc gactgctggg tatatcaaga
     5401 tctactattt acgtgaaaat aaatccggcc tcgaaatacc acgaccctac ctttcccaaa
     5461 ccaataaggc ttggtagtgc atccgtaggc tggcgggcat ctgccatcga tgagtggctg
     5521 atgttacata cggcaccagc ctggtctgaa cctgaaaaca aactaaagga acttaatgat
     5581 gacgacaaaa ctatcactcc ctgaacttga cacaactaaa ctgcctgaca ggtgccaggc
     5641 attacttgat gaaatgcatg aagaaacagg aatacgccgt gaaatacttc tgtctgttat
     5701 gctgacggtc atggctgcct cggtccagga tacgcatgaa gttgaacttt ccggagggca
     5761 acgtaccagc cttcagatat ttatgtgtct ttcatcagca tcaggcagtg gaaaaacctc
     5821 tgcctgcgca aaattaatcg ctcctgttca tgaaacagaa gaagaactac atcaggcata
     5881 tatcgacgat aaaaaaaact atgaccgaat gatggaaatg tggacaacag ataaaaaaat
     5941 cctggagcgg agatataaaa aggaaattga aagatccccg gaaaatgctg ctgctgcacg
     6001 agcagcactt gaaaagtgta tagcaaataa acccgaacct cccgtacaac aggttctcat
     6061 cgtgaatgac gcgacaccag aagggatagc tttgaaactc agccagtcac cctctcttct
     6121 gttgctgtct gatgaaggag gtactattct ggataagcgt ttcgaacgca aatcggcact
     6181 gtacaacaca ttatggagtg ggcaaccagt caacgttgaa cgggcatcca gacccgggtt
     6241 ccggctgaat gatgtccgtc tcactatact tatactgact caaccggtaa tatttaataa
     6301 gttttttact ctcactggcg atcagatccg gggcaatggc tttctggccc ggttgctatt
     6361 ttgcgaaccc ggagcaatta aaataatgac gacagaaccg catactgcca ctcctgttgt
     6421 gacgcaacaa tgtgcaagct gttttcgtaa gaaaagtttt ggcagtcaga tcagagactc
     6481 tctgagagca tccagagaaa gacgtgcaaa aagtaaagag cgcatctgca tgacattatc
     6541 aaatgcagca tcccgcgcac tggagcgctt tcacaaagaa aatatggata ctgtcagaca
     6601 gaatcctcat atgggtacat ttgaagacat tattgtcaga aaaagagagc aggttgtcag
     6661 gatagcagca cttctggaac tggagaaaga tccagactcg acagtaataa cgctggaaag
     6721 tactaatagc gccatttatc tcattgaatt ttatttcaag catcttatat ataaacttga
     6781 atcccttcgg gaaatatccc cggctgagaa acttgataaa tggcttcaga aaagaattat
     6841 cacaacagca ggttacatat tccagaaaag ttatatttta caatatgccc catatgctct
     6901 caggaaaaaa tgtgttctgg atgaagcact tgacatactg gcggaacaaa ggaaaataag
     6961 gattgatgat aacctggttg tttatatagg aaacacgatt acaccatccg aactggcaaa
     7021 aaaactaaat attcctgcat tcgatgccgg agttttcatt tgtgatcatc aaaacatact
     7081 gaagtatcac cgaaacagac tatagatact atcggacaaa aaataaatta tcacaagtgt
     7141 attattcgtt ataataatga ataatcgtta tctgagtgca atgccaggtt ttcattacga
     7201 actggcattg catttatttt tcaaaaaaga aagcagtaac catttggcta ctctgagtat
     7261 gccagtccat gaaactaagc gaattcctta gtgtttttag caagttgaga catcacataa
     7321 tccgcccatg cctgcatcat cggtacacgc tgctccagta gatcagtacg atgatatgcc
     7381 gcctcaacct tatttttcag cgtatgagcg agcgcccttt ccgccagatc ccgcgaatac
     7441 ccctgttcac tacaccagtc cctgaatgtt gagcgaaaac catgtgccgt ggcaactcgc
     7501 cccggaatgt cactgacggc tttcttttta cgcagaaaac ttgtcaacac catatcggaa
     7561 aggatctgct gctttctggg tgaagggaac accagttcat catgcaggcc acgtatattt
     7621 tccagaatgt aaatagcctg ccgggataaa ggaacacgat gctgtagcct agctttcatt
     7681 ctttctgcag gtatagtcca tacccgctta tgaaaatcaa tttcagccca gcgcattccc
     7741 ctggcttcgc ccgagcgagt tgctgtaagt atcaccatta ataacagtgc gcgggtaaca
     7801 ttataaggtt catcactata cacactggtc gccacaaaaa gcggtaactg cctccagggc
     7861 attgcgggtt ggtgttcatc acgtcctctt gtctgctgag gaagcaaatg gtcaaccaca
     7921 tcaacaggat ttgccacaca aaaaccgtgc gcccatcccc actgcataac aacatgaatg
     7981 cgctgtttaa cccggcttgc cgtttctgac aaggttaacc agactggacg cagtgtttct
     8041 gccacgtccg cagccgtaat cgaatccagc gtttttgctc ccagttgagg aaacgcgtaa
     8101 ttctcaagcg tcgataacca ctgccttaca tgctttggat tctcccatcc aggagacagt
     8161 tctgcatgta cacgcctggc tgcatcggca aatgttggga taacgacttt ctcagattca
     8221 gcctttttaa tctccagagg atcatcacct gcagcaagtt gctctcgcat tatccgggca
     8281 gtacgtgcag cttcagcaat actgacctct gggtaagttc ccaatccagc attacgtctt
     8341 ttttgtgtca ccggacttac ataacgaaaa acccatttcc cccgcccctt tactgaagaa
     8401 ggatgaaggg tcagtccggt aattccccca tggggcaatg gtttgtcatc aggtttgata
     8461 tgtcttgctt tcgtatccgt caatactgcc atacgctaat tcccgtcact ctggtatgcc
     8521 atccagtatg ccattagtcc ctcgcttcac taagaataca ttagataaca tcggacaaca
     8581 aaaagtgtaa tacattgttt ataaagaaat tatataacac ccctggatta agtcagattt
     8641 atttcaggcg gtccccctca ccgccatatt taaagaagag cgcgtacgaa agtacgggct
     8701 tttttttcgt atattgtaca caccgggtgc ccccgcccac gtctgttagg gcgaggggaa
     8761 attgtgcaca tcaactgtct ggttactcag tcaacaatca aaatgcgtaa gatagtacag
     8821 aacatatatg cgttatctgg tcacttcctg taacttcctc acttattaaa atgcccccaa
     8881 cggaaataca tgtcacatgc catcataaaa tcgttatgcc agaggaatcg acaatggaaa
     8941 aaatagtaaa ttaagcacgg tacatagcag agtaaaaaat aggcttcaaa ccaaatccgc
     9001 cattccacaa gcgttgccaa taccgacacc agcgtaatcc acatcatttc aataacccgc
     9061 aatatgaata accgaagcca aagcggcttg gcaaaaaagg tcgacaacgt attttcactg
     9121 gagaaaaaca gatcgatttc ctgcaaaaaa aacctgccca gaaaagcggg ccgcttggag
     9181 aagcgcagaa aaaacagatt aatcgccaac actacgccac aaagaatgag caatgcaatt
     9241 atcgatttaa acgttaacca ctgcccctct gtcacaaaag aatcactgtt acgcagcggg
     9301 ctgatagtaa gcaggttaac aaccgttgag atgcacaccg aaagcagcac atagcgagtc
     9361 agacttttat attttacttt tttcgctact gaaatagcga cctgcttgac ataactggtg
     9421 cggatcatcc gaaacagcag aaagatgacc aacggcccaa gtaacgaaat caggaacagc
     9481 gcatataacg agggtagaag ctggtatagc ccccaactca ttgcactcaa catgattaac
     9541 gacagaaatc ctgccaccag aggttgtgta agaaataatt ctattttgtg gttctgcttt
     9601 ttcttaaacc acgaaaaatc gatattcttc gtgacgcatt tataagccca gtacgccttg
     9661 agatcaaaaa taaaattata cagcaccagg gccagaatcc cactaactac accaatatcc
     9721 tctgatggaa aagtaaaccc tgcatttttc caataaaagc ctatcagaat aagtccaata
     9781 gcataaatgt atatcagatt tcgtttatcc tgaaagcgaa taaaagttaa ataatcagga
     9841 atcatttgaa tacctttgca tcactaaatg ctttacgaca atgcataact cgttgctgat
     9901 ccacgccagc aaaatcattt aacatattcc ccaaataggg cttattactt atttcaagga
     9961 aagctaccgg ggttggcata ttcagaatat gtatcgagag attcagtcca tttcaccgta
    10021 tgagttaagt gtaatgagag attttctttg ataattgacg gagcgacctc gctttttgct
    10081 gttacgttca tcagaacctg atgctctggc gaagcgatat ccagtccggc aagataatcg
    10141 cgcattgcct gaacgccgtc ttccatcaaa cgcgtgtgcc aggcaccgct cacaccgagt
    10201 ttaaccggtt cgtatccagc ggccatcagc agcgtggtaa attcattcaa cgcagcctgc
    10261 gttcccccaa taacctgctg gcgcggcgtg ttatcacagc taatatccag cgcaatgcct
    10321 gattccgtaa tcatcgtctg cagttgttcg cggttgatgc ctttaaccgc ctgatcttac
    10381 ccagcaatag tggacacgcg gctaagtgag taaactctca gtcagaggtg acttacatga
    10441 caaaaacagt atcaaccagt aaaaaacccc gtaaacagca ttcgcctgaa tttcgcagtg
    10501 aagccctgaa gcttgctgaa cgcatcggtg ttactgccgc agcccgtgaa ctcagcctgt
    10561 atgaatcaca gctctacaac tggcgcagta aacagcaaaa tcagcagacg tcttctgaac
    10621 gtgaactgga gatgtctacc gagattgcac gtctcaaacg ccagctggca gaacgggatg
    10681 aagagctggc tatcctccaa aaggccgcga catacttcgc gaagcgcctg aaatgaagta
    10741 tgtctttatt gaaaaacatc aggctgagtt cagcatcaaa gcaatgtgcc gcgtgctccg
    10801 ggtggcccgc agcggctggt atacgtggtg tcagcggcgg acaaggataa gcacgcgtca
    10861 gcagttccgc caacactgcg acagcgttgt
//
LOCUS       DAJTSI010000131        10813 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000131 DAJTSI010000000
VERSION     DAJTSI010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10813
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.131"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..621
                     /locus_tag="OO327_003132"
     CDS             <1..621
                     /locus_tag="OO327_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR6019574.1"
                     /translation="LVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKM
                     KPSDAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSP
                     FLGWLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIA
                     IACAAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            621..1397
                     /gene="lldR"
                     /locus_tag="OO327_003133"
     CDS             621..1397
                     /gene="lldR"
                     /locus_tag="OO327_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR6019575.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            1394..2584
                     /gene="lldD"
                     /locus_tag="OO327_003134"
     CDS             1394..2584
                     /gene="lldD"
                     /locus_tag="OO327_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR6019576.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            2780..3253
                     /gene="trmL"
                     /locus_tag="OO327_003135"
     CDS             2780..3253
                     /gene="trmL"
                     /locus_tag="OO327_003135"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR6019577.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(3306..4127)
                     /gene="cysE"
                     /locus_tag="OO327_003136"
     CDS             complement(3306..4127)
                     /gene="cysE"
                     /locus_tag="OO327_003136"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR6019578.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(4207..5226)
                     /gene="gpsA"
                     /locus_tag="OO327_003137"
     CDS             complement(4207..5226)
                     /gene="gpsA"
                     /locus_tag="OO327_003137"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR6019579.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(5226..5693)
                     /gene="secB"
                     /locus_tag="OO327_003138"
     CDS             complement(5226..5693)
                     /gene="secB"
                     /locus_tag="OO327_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR6019580.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(5756..6007)
                     /gene="grxC"
                     /locus_tag="OO327_003139"
     CDS             complement(5756..6007)
                     /gene="grxC"
                     /locus_tag="OO327_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR6019581.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(6149..6580)
                     /locus_tag="OO327_003140"
     CDS             complement(6149..6580)
                     /locus_tag="OO327_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR6019582.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            6825..8369
                     /gene="gpmM"
                     /locus_tag="OO327_003141"
                     /gene_synonym="pgmI"
     CDS             6825..8369
                     /gene="gpmM"
                     /locus_tag="OO327_003141"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR6019583.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            8379..9662
                     /gene="envC"
                     /locus_tag="OO327_003142"
     CDS             8379..9662
                     /gene="envC"
                     /locus_tag="OO327_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR6019584.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQHAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            9666..10625
                     /locus_tag="OO327_003143"
     CDS             9666..10625
                     /locus_tag="OO327_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR6019585.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(10612..10813)
                     /locus_tag="OO327_003144"
                     /pseudo
     CDS             complement(10612..10813)
                     /locus_tag="OO327_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         2555 a         2920 c         2922 g         2416 t
ORIGIN      
        1 ctggttgccc gtatgccacc agtggtcagc gaggctacag cctatgccgc cgtgtttaag
       61 tttgactggt tctctgccac cggcaccgcc attctgtttg ctgcactgct ctcgattgtc
      121 tggctgaaga tgaaaccgtc tgacgctatc agcaccttcg gcagcacgct gaaagaactg
      181 gctctaccca tctactccat cggcatggtg ctggcgttcg cctttatctc gaactattcc
      241 ggactgtcat caacactggc actggcactg gcgcacaccg gtcatgcatt caccttcttc
      301 tcgccgttcc tcggctggct gggggtattc ctgaccgggt cggatacctc atctaacgcc
      361 ctgttcgccg cgctgcaagc caccgcagca caacaaattg gcgtctctga tctgttgctg
      421 gttgccgcca ataccaccgg tggcgtcacc ggtaagatga tctccccgca atctatcgct
      481 atcgcctgtg cggcggtagg cctggtgggc aaagagtctg atttgttccg ctttactgtc
      541 aaacacagcc tgatcttcac ctgtatggtg ggcgtgatca ccacgcttca ggcttatgtc
      601 ttaacgtgga tgattcctta atgattgttt tacccagacg cctgtcagac gaggttgccg
      661 atcgtgtgcg ggcgctgatt gatgaaaaaa acctggaagc gggcatgaag ttgcccgctg
      721 agcgccaact ggcgatgcaa ctcggcgtat cacgtaattc actacgcgag gcgctggcaa
      781 aactggtgag cgaaggcgtg ctgctcagtc gacgcggcgg cgggacgttt attcgctggc
      841 gtcatgacac atggtcggag caaaacatcg tccagccgct aaaaacgctg atggccgatg
      901 atccggatta cagtttcgat attctggaag cccgctacgc cattgaagcc agcaccgcat
      961 ggcatgcggc aatgcgcgcc acacctggcg acaaagaaaa gattcagctt tgctttgaag
     1021 caacgctaag tgaagacccg gatatcgcct cacaagcgga cgttcgtttt catctggcga
     1081 ttgccgaagc ctcacataac atcgtgctgc tgcaaaccat gcgcggtttc ttcgatgtcc
     1141 tgcaatcctc agtgaagcat agccgtcagc ggatgtatct ggtgccaccg gttttttcac
     1201 aactgaccga acaacatcag gctgtcattg acgccatttt tgctggtgat gccgacgggg
     1261 cgcgtaaagc aatgatggcg caccttagtt ttgttcacac caccatgaaa cgattcgatg
     1321 aagatcaggc tcgccacgca cggattaccc gcctgcccgg tgagcataat gagcattcga
     1381 gggagaaaaa cgcatgatta tttccgcagc cagcgattat cgcgccgcag cgcaacgcat
     1441 tctgccgccg ttcctgttcc actatatgga tggtggtgca tattctgaat acacgctgcg
     1501 ccgcaacgtg gaagatttgt cagaagtggc actgcgccag cgtattctga aaaacatgtc
     1561 cgacttaagc ctggaaacga cgctgtttaa tgagaaattg tcgatgccgg tagcactggc
     1621 accggtgggt ttgtgtggca tgtatgcgcg tcgtggcgaa gttcaggcag ccaaagcggc
     1681 ggacgcgcat ggcattccgt ttactctctc gacggtttcc gtttgcccga ttgaagaagt
     1741 cgcgccagcc atcaagcgcc caatgtggtt ccagctttat gtactgcgcg atcgcggctt
     1801 tatgcgtaac gcgctggagc gagcaaaagc agcgggttgt tcgacgctgg ttttcaccgt
     1861 ggatatgccg acaccgggcg cacgctaccg tgatgcgcat tcaggtatga gcggcccaaa
     1921 tgcggcaatg cgccgctact tgcaggcggt gacgcatccg caatgggcgt gggatgtggg
     1981 cctgaacggt cgtccacatg atttaggtaa tatctcagct tatctcggca aaccgaccgg
     2041 actggaagat tacatcggct ggctggggaa taacttcgat ccgtccatct catggaaaga
     2101 ccttgagtgg atccgcgatt tctgggatgg cccgatggtg atcaaaggga tcctcgatcc
     2161 ggaagatgcg cgcgatgcag tacgttttgg tgctgatgga attgtggttt ctaaccacgg
     2221 tggccgccag ctggacggtg tactctcttc cgctcgtgcg ttgcccgcta ttgcagatgc
     2281 ggtgaaaggt gatatcgcca ttctggcgga tagcggaatt cgtaacgggc ttgatgtcgt
     2341 gcgtatgatt gcgctcggtg ccgacaccgt actgctgggt cgtgctttcc tgtatgcgct
     2401 ggcaacagcg ggccaggcgg gtgtagctaa cctgctaaat ctgatcgaaa aagagatgaa
     2461 agtggcgatg acgctgactg gcgcgaaatc gatcagtgaa attacgcaag attcgctggt
     2521 gcaggggctg ggtaaagagt tgcctacggc actggctccg atggcgaaag ggaatgcggc
     2581 atagtcgttt gcgccccctc accctaaccc tctccctcag ggagagggga ccgttcggcg
     2641 ctgtatgtac tccctcactc tgaaacgaca ccgcactctt tttttctccc tcgcccctcc
     2701 ggggagggcc ggggtgaggg gaaaaggccg cacggttcac caattttctg ctatcctgcc
     2761 cccgcactaa gggggcagta tgctaaacat cgtactttac gaaccagaaa ttccgccaaa
     2821 tactggcaac atcatccgtc tttgcgctaa taccggcttt cgtctacata tcatcgaacc
     2881 gatgggattt gcctgggacg acaagcgcct gcgccgcgcg gggctggact atcacgagtt
     2941 taccgccgtt acgcgtcatc atgactatcg cgcgttcctc gaagcagaaa atccccagcg
     3001 cctgtttgcc ctcaccacga aaggtacgcc tgctcacagc gccgtaagct atcaggatgg
     3061 cgattatctg atgttcggcc cggaaacacg cggcctgcca gcgagcattc ttgatgccct
     3121 gcccgctgaa caaaaaattc gcattccgat ggtgccggac agccgcagca tgaatctgtc
     3181 caatgcggtg tcggtagtgg tgtatgaagc ctggcggcag ctgggatatc cgggagcgtt
     3241 gttgagagat tagttactgt atgccggata gatgattaca tcgcatccgg cacgatcaca
     3301 ggacattaga tcccatcccc atactcaaat gtatggttaa taccgttgaa atgctggtcc
     3361 atatccattg atggcttatc gctgtctggt ttaccgacaa tacgagccgg aacgccagcg
     3421 gcggttgtat gcggcggcac cggttgcagc accacggaac ctgcgccaat cttcgcgccg
     3481 cgaccaactt caatattgcc gaggattttc gcgcccgcgc caatcatcac accttcacga
     3541 attttcgggt gacggtcacc accagattta cccgtaccgc caagcgtcac agattgcaga
     3601 atcgatacgt cgttttcaat taccgccgtt tcacccacaa cgatgcccgt ggcgtggtcg
     3661 agcataatgc cacggccaat ttttgctgcc gggtgaatat cgacctggaa cgtcacagaa
     3721 acctggtttt gcagaaagat tgccaatgcg cgacgcccct gattccacaa ccagtgaccg
     3781 atgcgatagg cctgcaaggc atgaaaaccc ttcaggtata acaacggggt tgagtattta
     3841 tcgactgccg ggtcgcgggt acgcaccgcc tgaatatcac aggccgcaga ggcgatcatt
     3901 tccgggtcag cggcgtaggc ttcttcaacc acttcacgga tagcaatagc aggcataatt
     3961 ggcgatgaca gcttgttcgc cagcatgtag ctcagtgcac tgccaaggtt ttcgtgcttg
     4021 agtagcgtcg cgtggtaaaa actggccagc attggctcac agtccgccag cgttctggct
     4081 tcggctttaa tattgttcca gacaatttcc agttcttcac acgacattgc ttactccaca
     4141 cgatgagata atgaccggcc cgttctgcgc gggccgggtc atagcggtaa caaaggttcc
     4201 ctggggttag tgactgctgc gctcgtcctt gcgtgcacga cctaataatg tcaatgctgc
     4261 ctcgcgcgcg ttttttccgc aatataatac ttgataaatt tcctcggtta ttggcatttc
     4321 aacgccgaag cgatgcgcca gttcgcggac ttctttcgta ttgcggtagc cttccaccac
     4381 ctgaccaatc ttctcctgcg cgctttgtac atccatgccc tgaccgagca tcataccaaa
     4441 acggcggtta cgcgactggt tgtcggtaca ggtaagcacc agatcgccca gccccgccat
     4501 gcccataaag gtggcaggat cggcacccag cgccgcacca agacgcgaca tttcagccag
     4561 cccacgggtg atcagcgccg tacgcgcatt cgcaccaaaa ccgataccgt cagacatccc
     4621 cgcgccaatg gcaatgacgt ttttcaccgc gccgccaagc tgcacgccaa tgaaatccgg
     4681 gttgctgtaa acgcggaaac ttttgccgca gtgcagcagc tgctggagat catcggcaaa
     4741 ggtctgatcg gtcgaggcca gcgaaatagc tgtcggtaaa cctgccgcca gttctttcgc
     4801 aaacgttggg ccagagataa ccgccagcgg aatttgatcg cctaaggcct cacgcgccac
     4861 gtcctgtaac agacgtccgg tttccgcttc cagccctttg gtcgcccaca ccagacgcgc
     4921 atcaggacgc atcagcggtt taatctggcg cagcacttca ccaaagacat ggctgggtac
     4981 gacgacgaga atattacgac tggctgccag cgcagtggcg agatcgcttt caagatggag
     5041 cgtatcggga aaaggcacat cggggagaaa cgcggcgtta cagcggtcgc gttcaagcgt
     5101 tgcgatatgt tcagggtcat ggccccagag gacaacctcg tggccatttc ttgccagggt
     5161 aatggcaaga gcggtgccgt acgagccggc accgatcaca gtcattgaag cattacgttg
     5221 gttcatcagg catcctgatg ttcttcagta ccttcgccag cctgctgctg caaatagttc
     5281 atgaacagcg catcgaagtt aaccggcgca aggttcagtt gcgggaatgt accgcgggat
     5341 accatgctgg tgatgcactc acgagcatac gggaacagaa tgttcgggca gtatgcgccc
     5401 aggcaatgtg ccatctgggt gccttcgata cccgcgatgg agaaaatacc gccctgctga
     5461 acttcacaca ggaacgcggt ttcttcgccc aaggaggccg ttacggtaac acgcagtacc
     5521 acttcgtata cgtcatctgc cagttgggaa gatgccgtat ccagatcaag tttaacttct
     5581 ggttgccaat ctttctggaa aacgtgcggc gcgttcggcg cttcgaaaga gatatccttg
     5641 gtataaatac gttggatctg gaaagtcatt tcagtgttgt tttgttctga catgtgtaga
     5701 aaacccttaa gtgttgtcct taaatactgc gcaatgtaat accaacgttc acacgttatt
     5761 tcagcagggg atccagtcca ccacgtgcat ccaatgcata caagtcatca cagccgccaa
     5821 tgtgctgtgc gtcaataaaa atctggggaa ccgtggtgca accgctgcgt ttgatcatct
     5881 cttcacgctt ggcggcattg ccatcgatcg gcaactcctg gaaactcacg cccttgctgc
     5941 tcagcagtgc ttttgcacga tggcaatacg ggcaggtttc tttggtatag atttcaacat
     6001 tggccataat ttagctcctg tttttttacc ctgaaatttc atgttgcagg gcggctgcaa
     6061 aatggagtgt ccccagaagc ttataacaat aagtcgctgg gggcctaatt gcagctaacg
     6121 gcctgcatca tgaaagacga caggtaaatt atttgccgcg caccagaggc aagttttcgc
     6181 cagcccagcc agcaacgccc tctttcagca cgaatacttg cgcaaaacca gctttcgtca
     6241 gcgcgtttgc aggctcctgg cactgcatgc cagaaccgtc taccacgata accgatttgt
     6301 ctttgtgctt ctcaagctca ccaacattgt tggctttgat ttcgctcggc aacagattga
     6361 tagaacctgc gatatggcct ttacggaagt catcacgctg acgtaaatcc acaaccacag
     6421 cgtcttcttt gttgatcaga cgcgtagctt caccacgagt aatcaccttc actttcgagg
     6481 tcaggctctt aaacgtagtc acaagaaccg ccaccagtaa cgcgatccag gcgatactca
     6541 gtatgggatg acggccaaca aattgcataa tttcttgcat ggggggtaac aactcccgac
     6601 gtagtgatta aaaaaaccag gaaaggagta tacctgcgcg gtgcggcaaa tacagccagc
     6661 gcgttaactg gaatgcaatt ttgcggggca cgacgaaaaa aaagcgcaaa tctggcatcg
     6721 gcttgccgcg agcggtcgta tattttgatc ttcagaggct attttatcga ttcagctgta
     6781 gtaaaattac gcaaattttg actcttgagt atgaggttgt cgcaatgtcg gtttctaaaa
     6841 aacctatggt actggtgatt ctggatggct atggctatcg tgaagaacag caggataacg
     6901 ccatttttag tgctaaaacc ccggtaatgg atgcactgtg ggccaatcgt ccgcataccc
     6961 tgatcgacgc ttccggtctg gaagtcggtc tgcctgaccg tcagatgggt aactccgaag
     7021 taggccacgt taacctgggt gccggccgca tcgtgtatca ggacctgact cgtctggacg
     7081 ttgaaatcaa agatcgcgct ttctttgcta atccagtgct ggctggcgcg gtacataaag
     7141 cgaaaaacgc aggtaaagca gtacacatta tgggtctgct ctccgcaggc ggcgtacaca
     7201 gccacgaaga tcacatcatg gcgatggtag aactggcagc tgaacgcggc gcagaaaaaa
     7261 tctacctgca cgcattcctt gacggtcgcg acaccccgcc gcgcagtgct gaatcctcgc
     7321 tgaaaaaatt tgaagagaaa tttgctgcgc tgggtaaagg ccgcgtagcg tccatcattg
     7381 gtcgttacta cgcgatggac cgcgataacc gttgggatcg cgttgaaaaa gcctatgacc
     7441 tgctgactct ggcgcagggt gagttccagg ccgatactgc cgttgctggt ttgcaggcag
     7501 cttatgctcg cgacgaaaac gacgagttcg tgaaagcgac cgttattcgt gcagaaggtc
     7561 agccagatgc ggcaatggaa gacggcgatg cgctgatttt catgaacttc cgtgctgacc
     7621 gcgcgcgtga aatcactcgt gcattcgtga acgctgattt cgatggcttc gcgcgtaaga
     7681 aagtggttaa cgtcgatttc gtgatgctga ccgaatacgc cgctgacatc aaaactgcgg
     7741 ttgcttaccc acccgcttcc ctggttaaca ccttcggcga gtggatggcg aaaaacgaca
     7801 aaactcagtt gcgtatttcc gaaaccgaaa aatatgccca cgttactttc ttcttcaacg
     7861 gtggcgtaga agagtcgttc aaaggcgaag atcgcattct gatcaactca ccgaaagtgg
     7921 ctacctacga tctgcaaccg gaaatgagct ccgcagagct gaccgaaaaa ctggttgcgg
     7981 ccatcaagag cggcaaatac gacaccatta tctgtaacta tccgaacggc gacatggtag
     8041 gtcacaccgg ggtgatggaa gcggcggtta aagcggttga agcactggat cactgcgtgg
     8101 aagaagtcgc gaaagcggtt gagtccgtgg gtggacaact gctgatcacc gctgaccacg
     8161 gtaacgctga acagatgcgc gatccggcaa cgggtcaggc acacacggca cacaccaacc
     8221 tgccagttcc gctgatttac gttggtgata agaacgtgaa agcggttgca ggcggcaaac
     8281 tttctgacat cgcgccgacc atgttgtcgc tgatgggtat ggaaatcccg caagagatga
     8341 ctggtaagcc gctgttcatc gtggaataat ccctccccat gaggggaaag gcgattaata
     8401 ccatgacacg ggccgtgaaa ccgcgcaggt ttgcaatcag gcccatcatc tacgccagcg
     8461 ttcttagcgc tggcgtattg ttgtgcgcct tttccgccca cgcggatgag cgtgaccaac
     8521 tcaaatctat tcaggccgat atcgccgcaa aagagcgcgc ggtacgccaa aagcaacaac
     8581 aacgcgcaag cctgctcgca caattgaaaa agcaggaaga agcgatctct gaagccaccc
     8641 gtaagctgcg cgaaacgcaa aacacgctca atcaactcaa taaacagatt gatgagatga
     8701 acgcgtcgat tgccaaactg gagcagcaaa aagccgccca ggagcgcagc ctcgccgcac
     8761 aactggatgc cgcattccgt cagggcgagc ataccggtat tcagctaatt ctcagcggtg
     8821 aagaaagcca gcgtggacag cgtttacagg cttatttcgg ctatctcaac caggcgcgac
     8881 aagaaaccat tgcccagttg aagcaaacgc gtgaagaagt caccatgcag catgctgaac
     8941 tggaagagaa acaaagcgag caacaaacgc tgttatatga acagcgcgcc caacaggcga
     9001 aactgactca ggctctgaac gagcgtaaaa agacgctggc ggggctggaa tcttccatcc
     9061 agcaaggtca gcagcagcta agtgaactgc gtgccaacga atcccgtctg cgtaacagca
     9121 ttgcccgtgc tgaagccgcg gcgaaagcgc gtgctgaacg agaagcgcgt gaggcccagg
     9181 cggttcgcga gcgccagaaa gaagcgacgc gcaaaggcac cacctacaaa ccgaccgaaa
     9241 gcgaaaaatc gctgatgtcc cgaactggtg gcctgggtgc gccgcgtggt caggcattct
     9301 ggccggttcg cgggccgacg ctgcatcgct atggcgagca gctacagggt gaattacgct
     9361 ggaaagggat ggttatcggt gcctctgaag gtactgaagt taaagcaatt gccgacggtc
     9421 gagtgattct ggctgactgg ctgcaaggtt acggtctggt ggtggtggtt gagcacggta
     9481 aaggcgacat gagtctttac ggctataatc agagcgcact ggtgagcgtt ggttcgcagg
     9541 ttcgcgcggg ccagccaatt gcactggtgg gcagcagtgg cggtcagggt cggccttcac
     9601 tctatttcga aattcgccgc cagggtcagg cggtcaatcc acagccgtgg ttgggaagat
     9661 aagttttgtt tccatttcgt cgtaacgttc ttgcatttgc cgctctgttg gcgctctcct
     9721 cccccgtact tgctggcaaa cttgccatcg tcattgatga ttttgggtat cgcccgcaca
     9781 acgaaaacca ggtgctggcg atgccttccg ctatctccgt cgctgtatta cccgattcac
     9841 cgcacgccag agagatggcg accaaagcgc ataacagcgg tcacgaagtg ttgattcatc
     9901 tcccgatggc accgttgagt aaacagccgc tggagaaaaa tacgctacgc ccggagatga
     9961 gcagcgacga aattgagcgc attattcgta gtgcggtcaa taacgtgccc tatgccgtgg
    10021 ggatcaacaa ccacatgggc agcaagatga cctctaacct gtttggtatg cagaaagtga
    10081 tgcaggcgct ggagcgttac aatctttact tcctcgacag cgtaaccatc ggtaataccc
    10141 aggcgatgcg cgccgcgcaa ggtactggcg tgaaggtgat caaacggaag gtgttcctcg
    10201 acgattcgca gaacgaagcg gacatccgtg tgcaatttaa tcgcgcaatt gatctggcgc
    10261 gtcgcaacgg ttcaaccatt gccattgggc atcctcaccc ttcaacggta cgcgtgctac
    10321 aacagatggt ttataacctg ccgccagaca ttacgctggt gaaagccagc agcttgctga
    10381 atgaaccgca ggttgatact tctacaccgc cgaaaaacgc tgtgcctgat gcaccgcgta
    10441 atccgttccg tggcgtgaag ctgtgcaaac cgaagaaacc gatagaacct gtttatgcta
    10501 atcgcttctt tgaagtatta agcgaaagca tcagccagag cacgctgatc gtttacttcc
    10561 agcatcagtg gcaaggctgg ggcaaacagc ccgaagcggc gaagtttaac gctagcgcaa
    10621 attaaaggcg ctacgcgtaa tgcgatggtg cgaccgcgtt ttgtcgcgcc attgccataa
    10681 gcggaaagac cacagtaacg cctggtaacc gaccttcacg ctgcgcacat tggtaatcag
    10741 gcgcttatac atacccggaa gtaaatatct cggcaatcat ccgttggcgg gtaagaatat
    10801 ccggctcttt gcg
//
LOCUS       DAJTSI010000132        10775 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000132 DAJTSI010000000
VERSION     DAJTSI010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10775
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.132"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="OO327_003145"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="OO327_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCR6019586.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="OO327_003146"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="OO327_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCR6019587.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="OO327_003147"
                     /pseudo
     CDS             complement(941..1408)
                     /locus_tag="OO327_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="OO327_003148"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="OO327_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR6019588.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="OO327_003149"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="OO327_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR6019589.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="OO327_003150"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="OO327_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR6019590.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="OO327_003151"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="OO327_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR6019591.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="OO327_003152"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="OO327_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR6019592.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="OO327_003153"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="OO327_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR6019593.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="OO327_003154"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="OO327_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR6019594.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="OO327_003155"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="OO327_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR6019595.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="OO327_003156"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="OO327_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR6019596.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="OO327_003157"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="OO327_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR6019597.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="OO327_003158"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="OO327_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR6019598.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="OO327_003159"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="OO327_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR6019599.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="OO327_003160"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="OO327_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR6019600.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="OO327_003161"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="OO327_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR6019601.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="OO327_003162"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="OO327_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR6019602.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="OO327_003163"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="OO327_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR6019603.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="OO327_003164"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="OO327_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR6019604.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="OO327_003165"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="OO327_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR6019605.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="OO327_003166"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="OO327_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR6019606.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="OO327_003167"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="OO327_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR6019607.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..>10775
                     /gene="rplO"
                     /locus_tag="OO327_003168"
     CDS             10518..>10775
                     /gene="rplO"
                     /locus_tag="OO327_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR6019608.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVE"
BASE COUNT         2866 a         2526 c         2853 g         2530 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgtgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc
      421 tgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atctgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
      781 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaatcg
      901 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
      961 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaaggc agcgggtttt
     1021 ttaatgattc ttttcctctc atgagcagac caataactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggtaagaagg cccatgaatg ctaggcgcca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgtcg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaacaa ttgcccccca taacgcatcg gctaaaccat
     1261 caggatgaca aacttgatag aacaaaagac ctgaccagag taacgggcac gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccaga aaaaaagtag ggctgataaa caagcgtaca
     1381 aaattagaaa gggaagggta gtgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagag agcgctgatt tgttaacctg attccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatgc agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcagaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctattgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtaca ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcttgg gtattgtaaa
     5221 accatggaac tctacttggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcatt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggaaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattattgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttacaaa ggcaaggggg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactcta caacaccctg
    10081 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10201 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcttgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaagtctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctatctgac ctggctaaag tagaa
//
LOCUS       DAJTSI010000133        10731 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000133 DAJTSI010000000
VERSION     DAJTSI010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10731
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.133"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(111..1775)
                     /gene="glnS"
                     /locus_tag="OO327_003169"
     CDS             complement(111..1775)
                     /gene="glnS"
                     /locus_tag="OO327_003169"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR6019609.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1978..3924)
                     /gene="nagE"
                     /locus_tag="OO327_003170"
     CDS             complement(1978..3924)
                     /gene="nagE"
                     /locus_tag="OO327_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR6019610.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            4257..5057
                     /gene="nagB"
                     /locus_tag="OO327_003171"
     CDS             4257..5057
                     /gene="nagB"
                     /locus_tag="OO327_003171"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR6019611.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5117..6265
                     /gene="nagA"
                     /locus_tag="OO327_003172"
     CDS             5117..6265
                     /gene="nagA"
                     /locus_tag="OO327_003172"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR6019612.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6274..7494
                     /gene="nagC"
                     /locus_tag="OO327_003173"
                     /gene_synonym="nagR"
     CDS             6274..7494
                     /gene="nagC"
                     /locus_tag="OO327_003173"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR6019613.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7542..8294
                     /gene="nagD"
                     /locus_tag="OO327_003174"
                     /pseudo
     CDS             7542..8294
                     /gene="nagD"
                     /locus_tag="OO327_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            8691..10355
                     /gene="asnB"
                     /locus_tag="OO327_003175"
     CDS             8691..10355
                     /gene="asnB"
                     /locus_tag="OO327_003175"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR6019614.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNVKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
BASE COUNT         2676 a         2770 c         2711 g         2574 t
ORIGIN      
        1 atgtaggcct gataagcgaa gcgcatcagg caatttagcg tttgaaattt attgtctatt
       61 tattgccgga tgcgacgtaa gtgcctcacc cggcttggta gaacttaaaa ttactcgcct
      121 actttcgccc aggtatcacg cagcccaacg gtgcggttaa ataccggttt ttcggccgtg
      181 gaatggcggc tgtcgaggca gaagtaacct tcacgctcaa actggaatgc tttacccgca
      241 accgcatctt tcagcgacgg ttcagcaaag ccctgtttga tcaccagcga ttccgggtta
      301 atcaccgaca ggaaatcatc cgcagcacct gggttaggca cgctgaacag acgatcatac
      361 aaacggattt caaccggcag cgcatgtgcc gcgctcaccc agtgaataac acctttgact
      421 ttacgaccat ctgccggatc tttgcttaag gtgtcggcgt cataagtaca gaagatggtg
      481 gtgatattac cttcggcatc tttctccacg cgttcagcct taatgacgta agcattacgc
      541 agacgcactt ctttacccag caccagacgt ttgtactgct tgttagcttc ttcgcggaaa
      601 tcggcgcgat cgatccaaat ttcaccgcta aacggcacct gacggctccc catttccggt
      661 ttgttcggat ggttcggcat ggtgaccatt tcgccttcgc cctgatagtt ttcgataacc
      721 agtttcaccg gatcgataac cgccattgcg cgcggcgcat tttcgttgag atcttcacgg
      781 atgcaggatt ccagcgacgc catctcaatg gtgttgtcct gcttggtcac gccgatgcgt
      841 ttgcagaact cacgaataga agccgcagtg taaccacgac gacgcagacc ggaaatggtc
      901 ggcatacgcg ggtcatccca gccttcaacg tgcttgtcgg tcaccagcag gttcagctta
      961 cgcttggaca tcacagtgta ttccagattc aggcgcgaga attcgtactg gcgcggatga
     1021 acaggaatgg tgatgttatc cagtacccag tcgtacagac gacggttgtc ctggaactca
     1081 agcgtacaca gagagtgcgt aataccttcc agcgcatcgc tgatgcaatg ggtgaagtcg
     1141 tacatcgggt agatgcacca cttgttgcca gtctggtggt gttcagcaaa tttaatgcgg
     1201 tacagcaccg gatcgcgcat tacgataaat ggcgacgcca tgtcgatttt cgcacgcagg
     1261 caggctttac cttcttcaaa accaccggca cgcatttttt cgaacagcgc caagttctct
     1321 tcaacgctgc ggtcgcggta cgggctgttt ttacccggct gcgtcagggt accgcggtat
     1381 tcgcggatct gttccggtgt cagttcatca acgtacgcca ggcctttatt gatcagttcg
     1441 atcgcatagg cgtggagctg atcaaaataa tcggaggagt aacggacgtt accagaccag
     1501 tgaaaaccta accactctac gtcgtttttg atcgaatcga catactcgat atcttctttt
     1561 accgggttag tgtcgtcgaa acgcaggttg cactggcctt tatagtcctg ggcgatcccg
     1621 aagttcaggc agatagattt cgcatggcca atatgcagat agccattcgg ctccggcggg
     1681 aaacgggtgt gtactgtgat gtgcttacca ctggccagat cttcatcgat gatctgacgg
     1741 ataaagttag tcgggcgggc ttctgcctca ctcatcgtgg attcctcaaa gcgtaaacaa
     1801 cgtataacgg cgtatgatct tataagcggg acaggctgac aactgttagt tttttaaaat
     1861 tgataatcta acaaggatta tggggatgat tgatgaaaaa aaaagcggca gagaagatct
     1921 ccaccgcttg tcattgttgg atgcgacgct gccgtgtcgc atcaggcata aagcagatta
     1981 ctttttgatt tcatacagcg gtgtttgacc cgccacaaca tggccctgag ctttaatgag
     2041 caagccactg aaatcgtcga tattgctgca aaccaccggg ctaatcatcg agcgggcgtt
     2101 agcgttcagg taatccagat ccatttccag aatcggttgc cctgcgctta cctgcgcacc
     2161 ctcttccacc agacgtttaa agcctttacc ttccagcgct acggtgtcga tacccatatg
     2221 gacgacgatc tccgcgcctt tttcggtttc caggcagaac gcgtggttgg tgttgaagat
     2281 tttcacgatt gtccctgcgg ctggtgatac gacgatttta tctgtcggtt tcaccgccac
     2341 accgtcaccc accgctttgc tggcgaatgc ttcgtcagga acctgatcca gtgccacgac
     2401 atcaccggta atcggcgata ccagctccgc gatagatacc gcgtttggta cagcctgcgg
     2461 ttttgctaca ggcgcggcag ttgccggagt tgcttcagct gacgcagcgg ctaccggacc
     2521 acgggcaacg actttcttca tcgcatcgcc gatggattct gctttcgcgc caacaatcac
     2581 ctgaatagtc tgtttgttca gtttcactac accagaagca cccagacgtt tacacatcgt
     2641 atcgttgacg cgggcagagt cagccacggt caggcgcaga cgggtaatac aggcatcaat
     2701 cgctttcagg ttgtcagtac cgccaaccgc agcaatatag ttggttgcca gttgagtcag
     2761 accttcttca gtattgctgt tggcttcttc agtaacgatc tcgtcttctt tatcttcacg
     2821 acccggcgtt ttcaggttga acatgcggat aaccaaactg aacaccacga agtagatagc
     2881 gaagaagata acgcccatca ccagcagcat ccagacgttc tggctggcgg caggcaggtt
     2941 atacatcaac gcgtagtcga tagcacccgc agagaaagag aagccggcat ggatacccag
     3001 cagcgttgcc acaaacaggc tgataccggt cagcagtgcg tgcaggaggt acagcagcgg
     3061 cgcaaggaac atgaacagga attccagcgg ctcagtcaca ccggtcagga acgcagtaac
     3121 agcaacagaa agcagcatcc cgccaaccat cggacgacgc tctttcggtg ctgcgaagta
     3181 catcgccagc gccgcacccg gcagaccgaa catcatgatc gggaagaagc cggacatgaa
     3241 catccccgcg gtgccgtcac cagcgtagaa acggttgatg tcgccgtgga aaaccgtacc
     3301 cgccgcgttg gtgaattcac caatctggaa ccaggcgatg gtgttcagca cctgatgcag
     3361 accggttggg atcagcagac ggttgatgaa accaaagata ccggaaccca gcgcgcccgc
     3421 agaaacgatc cactcgccgc ctgcatggat agcgtgctgt accggcggcc agacgtaacc
     3481 aaaaatggcc gccagcacca gacagaagaa gccggtggcg atcggcacaa agcgtttgcc
     3541 gccgaagaag ctcaggaagt ccggcagttt aatatcggac caacggttat aggctgcgcc
     3601 accaaccaga ccggtaatga tacccgccag tacacccatg ttaatttctg ggttgatggt
     3661 caccatcgct ttggttaaca caaagtaacc taccgcaccc gccagcgccg ccgcacctgc
     3721 gctgtctttc gaccagctgg atgccacacc gatggcgaag attaatgcga ggttatcaaa
     3781 aattgcaccg cccgcctggg caataaacgc aacgttaagt aaatctggct gaccgaatcg
     3841 cagcaacagc gccgccaccg gcagcaccgc gatagggagc tgtaacgccc taccgagtcg
     3901 ctggaaaaaa cctaaaatat tcatcttatt ccccctacga gaaccctatt tggctcgttt
     3961 aaagccgtat ttttattttg ctgcaaattt tactgccgat gttctgtaat cagattgtta
     4021 gatcatctgc tacagagtgt gtgaaaattt aattcgcatc gcaaattaaa cgcgtgtctt
     4081 ttgtgagttt tgtcaccaaa tatcgttatc atcactccct tttactggct aaaccagaaa
     4141 acttatttta tcattcaaaa aatcaagtcg gattgacgcc tgtctgcgca aatccaggtt
     4201 acgcttaaag atgtctaatc cgccaacggc ttacatttta cttattgagg tgaataatga
     4261 gactgatccc cctgactacc gctgaacagg tcggcaaatg ggctgctcgc catatcgtca
     4321 atcgtatcaa tgcgttcaaa ccaactgccg atcgtccgtt tgtactgggc ctgccgactg
     4381 gcggcacgcc gatgaccacc tataaagcgt tagtcgaaat gcataaagca ggccaggtca
     4441 gctttaagca cgttgtcacc ttcaacatgg acgaatatgt cggtctgccg aaagagcatc
     4501 cggaaagcta ctacagcttt atgcaccgta atttcttcga tcacgttgat attccagcag
     4561 aaaacatcaa ccttctcaac ggcaatgccc cggatatcga tgccgagtgc cgccagtatg
     4621 aagaaaaaat ccgttcttac ggaaaaattc atctgtttat gggcggtgta ggtaacgacg
     4681 gtcatattgc atttaacgaa ccggcgtctt ctctggcttc tcgtactcgt atcaaaaccc
     4741 tgactcatga cactcgcgtc gcgaactctc gtttctttga taacgatgtc aatcaggtgc
     4801 caaaatatgc cctgactgtc ggtgttggta cactgctgga tgccgaagaa gtgatgatta
     4861 tggtgctggg tagccagaaa gcactggcgc tgcaggccgc cgttgaaggt tgcgtgaacc
     4921 atatgtggac catcagctgt ctgcaactgc atccgaaagc gatcatggtg tgcgatgaac
     4981 cttccaccat ggagctgaaa gttaagactt taagatattt caatgaatta gaagcagaaa
     5041 atatcaaagg tctgtaattg ttatccctgc cctggttcct tgctcagggc aatatttttt
     5101 aaaatcgggg ggcagaatgt atgcattaac ccagggccgg atctttaccg gccacgaatt
     5161 tcttgatgac cacgcggttg ttatcgctga tggcctgatt aaaagcgtct gtccggtagc
     5221 ggaactgccg ccagagatcg aacaacgttc actgaacggg gccattctct cccccggttt
     5281 tatcgatgtg cagttaaacg gctgcggcgg cgtacagttt aacgacaccg ctgaagcggt
     5341 cagcgtggaa acgctggaaa tcatgcagaa agccaatgag aaatcaggct gtactaacta
     5401 tctgccgacg cttatcacca ccagcgatga gctgatgaaa cagggcgtgc gcgttatgcg
     5461 tgagtacctg gcaaaacatc cgaatcaggc gttaggtctg catctggaag gtccgtggct
     5521 gaatctggta aaaaaaggca cccataatcc gaattttgtg cgtaagcctg atgccgcgct
     5581 ggtcgatttc ctgtgtgaaa acgccgacgt cattaccaaa gtgaccctgg caccggaaat
     5641 ggttcctgcg gaagtcatca gcaaactggc aaatgccggg attgtggttt ctgccggtca
     5701 ctccaacgcg acgttgaaag aagcaaaagc cggtttccgc gcggggatta cctttgccac
     5761 ccatctgtac aacgcgatgc cgtatattac cggtcgtgaa ccgggcctgg cgggcgcgat
     5821 cctcgacgaa gctgacattt attgcggtat tatcgctgat ggcctgcatg ttgattacgc
     5881 caacattcgt aacgctaaac gccttaaagg cgacaaactg tgtctggtta ccgacgccac
     5941 cgcgccagca ggtgccaaca ttgaacagtt catttttgcg ggtaaaacaa tatactaccg
     6001 taacggactt tgtgtggatg agaacggtac gttaagcggt tcatccttaa ccatgattga
     6061 aggcgtgcgt aatctggtcg aacattgcgg tatcgcactg gatgaagtgc tgcgtatggc
     6121 gacgctctat ccggcgcgtg cgattgacgt tgaaaaacgt ctcggcacgc tcgccgcagg
     6181 taaagtagcc aacctgaccg cattcacacc tgattttaaa atcaccaaga ccatcgttaa
     6241 cggtaacgag gtcgtaactc aataagagaa agtatgacac caggcggaca agctcagata
     6301 ggtaatgttg atctcgtaaa acagcttaac agcgcggcgg tttatcgcct gattgaccag
     6361 tacgggccaa tctcgcggat tcagattgcc gagcaaagcc agcttgcccc cgccagcgta
     6421 accaaaatta cgcgtcagct tatcgaacgc gggctgatca aagaagttga tcagcaggcc
     6481 tccaccgggg gccgccgcgc tatctccatc gtcaccgaaa cccgcaattt ccacgcaatc
     6541 ggcgtacggc ttggtcgtca tgacgccacc atcactctgt ttgatctcag cagcaaagtg
     6601 ctggcagaag aacattaccc gctgccggaa cgtacccagc agacgctgga acatgccctg
     6661 ctgaatgcca ttgctcagtt tattgatagc taccagcgca agctgcgcga gctaatcgcc
     6721 atttcggtga tcctgccagg gcttgttgac ccggacagcg gcaaaattca ttacatgccg
     6781 catattcagg tagaaaactg ggggctggta gaagcactgg aagaacgttt taaagtgacc
     6841 tgtttcgttg gtcacgatat ccgtagtctg gcgctggcgg agcactactt cggtgcaagt
     6901 caggattgcg aagactccat tctggtgcgt gtccatcgcg gaaccggggc cgggattatc
     6961 tctaacgggc gcatttttat tggccgcaac ggtaacgtcg gtgaaattgg ccatattcag
     7021 gtcgaaccgc tgggtgaacg ctgccactgc ggcaactttg gctgcctgga aactatcgcc
     7081 gccaacgctg ccattgaaca acgggtgttg aatctgttaa agcagggcta ccagagccgt
     7141 gtgccactgg acgactgcac catcaaaact atctgcaaag ccgcgaacaa aggcgatagc
     7201 ctggcctcgg aagtgattga gtatgtcggt cgtcatctgg gtaaaaccat cgccattgct
     7261 atcaacttat ttaatccgca aaaaattgtt attgctggtg aaatcaccga agccgataaa
     7321 gtgctgctcc ctgctattga aagctgcatt aatacccaag cgctgaaggc gtttcgtact
     7381 aatctgccgg tggtacgttc tgagctggat caccgctcgg caatcggcgc ttttgcgctg
     7441 gtaaaacgcg ccatgctcaa cggtattttg ctccagcatt tgctggaaaa ttaatgtgct
     7501 tttatagtgg cgcttattgt tgtcaatatt ctgggtagtc catgaccatt aaaaatgtaa
     7561 tttgcgatat cgacggcgtg ctgatgcacg ataacgtcgc cgtaccgggt gcagcggaat
     7621 ttttgcacgg gattatggat aaaggcctgc cgctggtgtt gctgaccaac tatccttcgc
     7681 agactgggca agatctggcg aaccgctttg ccactgccgg tgtcgatgtg ccggatagcg
     7741 tgttttatac ctctgcgatg gcgaccgccg atttcctgcg tcgctaggaa ggcaagaaag
     7801 cgtatgtggt gggcgaaggc gcactgattc atgaactgta caaagccggt ttcactatta
     7861 ccgatgtgaa ccctgatttc gtgattgttg gcgaaacacg ttcctacaac tgggacatga
     7921 ttcataaagc agcctatttc gtcgctaacg gtgcacgctt tatcgccacc aacccggaca
     7981 cccacgggcg cggtttttat cccgcttgtg gcgcgttgtg tgcagggatt gagaaaatct
     8041 ccgggcgcaa accgttctat gttggtaagc ccagcccgtg gatcatccgc gcggcattaa
     8101 acaaaatgca ggcgcattcg gaagaaacag tgattgtcgg cgataacctg cgtaccgata
     8161 ttctggcggg cttccaggcc ggtctggaga cgattctggt gctttctggt gtttcatcgc
     8221 tcgacgatat cgacagtatg ccgttccgcc caagctggat ttacccgtca gtcgctgaaa
     8281 tcgacgttat ctgaatacca cccacgtctg cggacgtggt tggctctgat gacaaacgcc
     8341 aaactgcctg atgcgctacg cttatcaggc ctacgcagct cctgcaatat attgaatttt
     8401 catgcttttg taggccggat aaggcgttca cgccgcatcc ggcatgaaca aatcgcactt
     8461 tatcagcaat ttggcccccg taaaatataa ccattcaata aaacccgcta aaaattatcg
     8521 attcatcaat aaatacatcc tcacaccaga ccctttttca ccaatcgaca atcaccatta
     8581 cgttttttat ttttgcactg ccaaatagct tgcgcacagt gctgctttgc ggcatttttt
     8641 taaacaagca aacacaacaa gcaacaaata ccaggttaac ggagaaggtt atgtgttcaa
     8701 tttttggcgt attcgatatc aaaacagacg cagttgagct gcgtaagaaa gcactcgagc
     8761 tgtcacgcct gatgcgtcat cgtggcccgg actggtccgg tatttatgcc agcgataacg
     8821 ccattctcgc tcacgaacgt ctgtcaattg ttgacgttaa cgcgggggcg caacctctct
     8881 acaaccaaca aaaaaaccac gtactggcgg taaacggtga aatctacaac caccaggcat
     8941 tgcgcgctga atatggcgat cgttatcagt tccagacagg gtctgactgt gaagtgatcc
     9001 tcgcgctgta tcaggaaaaa gggccggaat ttctcgacga cttgcagggc atgtttgcct
     9061 ttgccctgta cgacagcgaa aaagatgcct acctgattgg tcgcgaccat ctggggatca
     9121 tcccactgta tatggggtat gacgaacacg gtcagctgta tgtggcctca gaaatgaaat
     9181 cgctggtgcc agtttgccgc acgattaaag agttcccggc ggggagctat ttgtggagtc
     9241 aggacggcga aatccgttct tattatcatc gcgactggtt cgactttgat gcagtgaaag
     9301 ataacgtgac cgacaaaaac gagctgcgtc aggcactgga agattccgtt aaaagccatc
     9361 tgatgtctga tgtgccttac ggtgtgctac tttctggtgg tctggattcc tcaattattt
     9421 ccgctatcac caagaaatac gcagcccgtc gcgtggaaga tcaggaacgc tctgaagcct
     9481 ggtggccgca gttacactcc tttgctgtag gtctgccggg ttcaccggat cttaaagcag
     9541 cccaggaagt ggcaaaccat ctgggcacgg tgcatcacga aattcacttc actgtacagg
     9601 aaggtctgga tgccatccgc gacgtgattt accacatcga aacttatgat gtgaccacta
     9661 ttcgcgcttc aacaccgatg tatttaatgt cgcgtaagat caaggcgatg ggcattaaaa
     9721 tggtgctgtc cggtgaaggt tctgatgaag tgtttggcgg ttatctttac ttccataaag
     9781 cgcccaacgt taaagaactg catgaagaga cggtgcgtaa actgctggcc ttgcatatgt
     9841 atgactgcgc gcgcgccaac aaagcgatgt cagcctgggg cgtggaagca cgcgttccgt
     9901 tcctcgacaa aaaattcctt gatgtggcga tgcgcattaa cccgcaggat aaaatgtgcg
     9961 gtaacggcaa aatggaaaaa cacatcctgc gtgaatgttt tgagtcatac ctgcccgcaa
    10021 gcgtggcctg gcggcagaaa gagcagttct ccgatggcgt cggttacagt tggatcgaca
    10081 ccctgaaaga agtggcggcg cagcaggttt ctgatcagca actggaaact gcccgcttcc
    10141 gcttcccgta caacacgccg acctcaaaag aagcgtatct gtaccgcgag atctttgaag
    10201 aactgttccc acttccgagt gccgctgagt gcgtgccggg cggtccttcc gtcgcgtgtt
    10261 cttcagctaa agcgatcgaa tgggatgaag cgttcaaaaa aatggacgat ccgtctggtc
    10321 gtgcggttgg cgttcaccag tcggcgtata agtaagaaat gcggtactgc cccggagaaa
    10381 tccggggctg ttttagttat tgcgctgctt gttgattctg atatgcagta aggaaatgtt
    10441 ccgggtttgc cacatcactg tttgacaact catcactgta ggccagtatt tcatagatta
    10501 atttcccacc actattgttg aattccgaat ttcatcctgt acgacaaaag aatggctgta
    10561 gggtggggaa aaacacaaag taaacaataa ttgacgaata tagcgccacg ctgttcgcaa
    10621 cctaaccaaa cagtcacttt cgagcaattt tccttgaaaa agaggttgac gctgcaaggc
    10681 tctatacgca taatgcgccc cgcaacgccg ataaggtatc gcgaaaaaaa g
//
LOCUS       DAJTSI010000134        10464 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000134 DAJTSI010000000
VERSION     DAJTSI010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.134"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            3..203
                     /gene="cspG"
                     /locus_tag="OO327_003176"
     CDS             3..203
                     /gene="cspG"
                     /locus_tag="OO327_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001990088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCR6019615.1"
                     /translation="MTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRTLNEN
                     QKVEFSIEQGQRGPAAANVVTL"
     gene            214..402
                     /locus_tag="OO327_003177"
     CDS             214..402
                     /locus_tag="OO327_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR6019616.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            377..607
                     /gene="ymcE"
                     /locus_tag="OO327_003178"
     CDS             377..607
                     /gene="ymcE"
                     /locus_tag="OO327_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HCR6019617.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGKM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            597..770
                     /locus_tag="OO327_003179"
     CDS             597..770
                     /locus_tag="OO327_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCR6019618.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(819..1892)
                     /locus_tag="OO327_003180"
     CDS             complement(819..1892)
                     /locus_tag="OO327_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR6019619.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1994..4705)
                     /gene="torS"
                     /locus_tag="OO327_003181"
                     /pseudo
     CDS             complement(1994..4705)
                     /gene="torS"
                     /locus_tag="OO327_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            4788..5816
                     /gene="torT"
                     /locus_tag="OO327_003182"
     CDS             4788..5816
                     /gene="torT"
                     /locus_tag="OO327_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCR6019620.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5789..6481)
                     /gene="torR"
                     /locus_tag="OO327_003183"
     CDS             complement(5789..6481)
                     /gene="torR"
                     /locus_tag="OO327_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCR6019621.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6568..7740
                     /gene="torC"
                     /locus_tag="OO327_003184"
     CDS             6568..7740
                     /gene="torC"
                     /locus_tag="OO327_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCR6019622.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7740..10280
                     /gene="torA"
                     /locus_tag="OO327_003185"
                     /pseudo
     CDS             7740..10280
                     /gene="torA"
                     /locus_tag="OO327_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10277..>10464
                     /gene="torD"
                     /locus_tag="OO327_003186"
     CDS             10277..>10464
                     /gene="torD"
                     /locus_tag="OO327_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR6019623.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         2524 a         2685 c         2788 g         2467 t
ORIGIN      
        1 aaatgactgg tttagtaaaa tggtttaacg cagataaagg ttttggcttt atcactcctg
       61 atgatggcag caaagacgtt ttcgtccatt tcaccgccat ccagagcaat gaattccgca
      121 cgctgaacga aaatcagaaa gttgaatttt ctattgagca ggggcaacgt ggccccgcgg
      181 cagcgaacgt tgttacgctc taaggttgcc gttattactc aacatctcca tttccgctgt
      241 ccatgttgtc atggttcaca gtaccgtaca tcggcattcg atgtgacgga gcgaaaccct
      301 ttgggcgcta agtgtatttt ttgtaaatcg actatgatca cctttgataa cgtcgcgctg
      361 caaatacgca ctgaccatgc gccgctggat ttcacaaaat aatatcaggc tccctcgtgg
      421 agcctttttt atatctgcct tatttttctt caacgctgta tgtatagtaa gcgataacct
      481 gttgattatt gaatctttcg ggaagatggc ttataacatt tcttacctga ccagggtacc
      541 gggaaccaac accttactgg cgtgttgctg tctttcaaga ccagacgagg ttaacagtga
      601 atattgaaga gttaaaaaaa caagccgaaa cggaaatcgc cgactttatc gcgcaaaaaa
      661 tcgccgagct gaacaagaat acagggaaag aagtctctga aattcgcttc atcgcacgag
      721 aaaaaatgac cgggcttgaa agttatgatg tcaaaatcaa aataatgtga ttttgtgaac
      781 atcaccccgt gcgaggtgat gttccgcttg ttgctaattt agtgaccaat cattggcgct
      841 tgtggaatta agcgtcggta caattcctcc ggcaccgggc tttgccatac tcccgcatac
      901 attgcgtaac caatcaccgc aaacataatc cccagaacca gtagcgtcat taaccagcca
      961 gacaacgcaa aggctttttt atttgctgca ggtttttgca gtgaaaaggt caatgttgag
     1021 gctaccggac atgactctac gcaagtcata cagccggtac attccactgt tcgtacctga
     1081 attaatttat ctaccgggat ccgtgatggg cacttttttg cgcatttgcc acaatcgata
     1141 caactttcgg cattgcgacg aatcttaaac ggcgacaata gcgaaaccac gcccatcagc
     1201 gcgccatatg ggcaaagata acgacaccag gcatggcgaa taaacaggct ggcaatcagc
     1261 aaaacggtca cgctgattaa tgtcgcggtc cccatatgac gaaagaaatc gagcatttta
     1321 acgtccatca ccacgctata gggcgacaac ataaaatagt gaatcgcctg agcgggcatc
     1381 aataacgcga tatagagaaa taaactcaac agcaaatact tcacgccgcg cagaggaata
     1441 tccagccagc ggggaaggac acattgccga ccaaacagtt tgttaccgag atcgccgatt
     1501 aattcagaaa gcgtaccaac cgggcataac catgagcaaa aggccttttt gagtaataga
     1561 ctgatgacga taaaagcgac caataacagc atcgcggcgg cgtggacgga cggtaactga
     1621 cctgttgcaa ggctatattt cagattcatc agaccggcaa tcggtagcca gccttcgata
     1681 cctcccggtc tggcgacaaa tgtcgtacta cctgccgttt cgtaatagcg cacccaatac
     1741 cagaacgtga tggcaatata aatattcatt gccaatagta agaattgcgt cgctttacgc
     1801 caggtcgtgg cattgcgcca gtcattccac ggtaatttgc cgcccgtcgt gcctggccgc
     1861 cgctgccagc gggttctttt tttctctgcc atgattttgc cagtccgtta agttgcatac
     1921 caaatgccac tattctagtt attcttaact ggctgatatt gattcaaatc gcgttcaggt
     1981 ctttcttatt caatcatgct tccagagcgg caacactgcg tgtaatttct tcgtgtggaa
     2041 ggggggcaga taatggctgc tgctccagtt gtgcgcatag ctgactggcg ctacgcattc
     2101 ccagactcga gcaactgctt tttagctgat gcgcggcacg ctttattttc tcgctgtcct
     2161 ggctggcgcg ggcaatgtcg atttcatcga gaagcggcag ggcatgttgt ttaaataatg
     2221 ccagccattc gtggatcttc tccgtcccca ttaactgagc gtcttcattg agttgcgaga
     2281 catccagtgg tagatcgtta ttggcttgca gttggagata gtgcgccagt aactgaccga
     2341 gcacttcacg cggcaccggt ttagggataa tcccgcgaaa tagtgaactg gtacgctggc
     2401 gcagcgtttc gtcaatgaca tgggcgctaa agccaatcaa aaccagcgac ggatattgct
     2461 gtgccagttg ccgggcaagg gtaatgccgt cgatatccgg cagatcaaaa tccaccagtg
     2521 cggcagcaaa cggttcgcta ttttgcagtg tctctaaagc ctgcgcggca ttgccaatag
     2581 caacaacctg cgcaccactg gtgttcagca tctcaacggt aattcgctgg gttagcgggt
     2641 tatcttcaat taacagcaaa cgtaaaccgt caagacgcac cgcctgattg gctgtttttg
     2701 gcacgggtgc agtggcaaca cgtaacggca agcgtaaaca aaaacagctt ccaacctccg
     2761 gcgtgctggt ggcgctcagt tcgccgccca tcgcctgggc cagacggcta ctgatagtca
     2821 gccccagccc ggtgccgccg cgtttgccgc ttacctgcac aaatggctgg aagatttctg
     2881 ccagtttcgc gggatcaata ccgcagccgc tgtcttccac ttcgaccagc cattgctcgc
     2941 catcagtgcg actacgcagg ataatgtgcc cttcgtcagt aaaacgcagg gcattgctca
     3001 ataggttggt aatgacctga cgaatacgtc gtggatcgcc cattaacgcg ctcggtacat
     3061 catcggcaat tgccgttgcc aggcgaatcg ggcgaccttt cacccgtccg ctcattaatt
     3121 gcagggtact ttccagcagc gggcgcggtt caaagggctc atcgctgact gaaacatttt
     3181 tgccacctgc ttcgatagcc gaataatcga gaatatcgtt gaggatggtc agcaacgatt
     3241 cgccagagtc ggtaatcgcc tgcaaatcat cacgctgggc gttaagtgcg gggttatctg
     3301 ccagcagttg agcagtgccg agaataccgt acagcggtgt gcggatctca tggctcatcg
     3361 ccgtcagaaa cgccgatttt gcctggctgg ctttttctgc ttccgcccgt gcctgtcggt
     3421 gttctatcac cagttcctgc aattcagctg tacgcgcttt gacctgcgcc gccagctgtt
     3481 cgcggtggcg attcagtgca tgaacactgc tgcgaaacgc atccatcagc cgcccgatgg
     3541 tatccagctc ccgtacgcca gcggtttccg ggaaagggga gtcaatatca ccgtccagca
     3601 gccgttgcag cgcctgcgtt tgttcggcaa gtggacgcgt gaatgagcga taaaccacgc
     3661 gccagaggat cataatcagt gcgcaaagtg aaaccatccc cagcaataac aggctgtatt
     3721 gcccgcgtgc actggctttt tccagatgtg ccagtccatg ctgattgtgc agctcaatgg
     3781 tgtcgaccag ctgactgact tcgctactaa actgcgcgaa ctgggcgata ttattttggg
     3841 cgagagtttg caggtgattg ctgatttcac tgtcctgctg gaacagcgcc aacagatcgc
     3901 tatattggct aacggtagtt aacgttgttg cgacctgcgc acgaactccc ggatcttcaa
     3961 tgcgtatttg ccgacgttgc agaattttca ccgcattatt gagctgcttt tccagcgttg
     4021 gtgcattttt ctggatctgc tccagcccca gattcatcac catttgctgc acccgcagag
     4081 cgctaaggcg cagttcattc atctggttaa catactcaag atcgatatca atcagccgat
     4141 cgagtgcact ttcagcagcc tgacgctgat cttgttcgat caaatcgtaa atcccggcct
     4201 gggtcgctcc agcggaagtt gtcgcattat tcgcctgacc ttgctccagg cgtgcgatct
     4261 catcggcggc agctatctgc tgactgagtt gccgttgttg ctggcgtagt tgcagacgcc
     4321 gccccaccag ttccccttgc tgacgtaatg aacgggagat ctcctgctcc tgttgttcaa
     4381 tagcggtggt atcaaaacct tgttcccgta acgcttgcag caacgcatta atcttcaggc
     4441 tttatgcggt gagcattcgc ccctgcgcct gccacatctt ttcgttatcg gcactggtca
     4501 ggttctgcgc ggcgaaaagt tcccaggcgc tggcttcgct caactggcgc gccatattca
     4561 tggtaggaat caatgcctga gtattgtctt tttccacctg gctgataaag cgcaggttgt
     4621 accatcccac cagggtactg gtcagggtta acagcgccat cagggcaaag cccatccaga
     4681 gtcttcgggt cagggttaaa ttcacggtcg gtgcacttaa ggtgaaaaag gttgagtcgc
     4741 aaagtggaat gcatctagca taaagcctta ttattgatga ggctatcatg cgcgtactgc
     4801 tatttttact tctttccctt ttcatgttgt cggcattttc ggctgataac ctgttgcgct
     4861 ggcatgatgc gcagcatttc acggtgcaag cctctatgcc gcttaaagcc aaacgcgtat
     4921 ggaaactgtg cgcgctttat cccagcctga aagattcata ctggttatcg ttgaactatg
     4981 gtatgcagga ggctgctcgc cgctacggtg tggatttaaa agtgctggag gcaggcggct
     5041 acagccagtt ggctacccag caagcacaaa tcgaccagtg taaacagtgg ggcgcagagg
     5101 ccattttgct cggtagtagc acgacgtcat ttcccgacct gcaaaagcag gtagcaaatc
     5161 tgccggtgat cgaactggta aatgctcttg atgatcccca ggtgaaaagc cgcgttggtg
     5221 taccctggtt tcagatgggc tatcaacctg ggcgatattt ggtgcaatgg gcgcacggta
     5281 aaccactgaa cgtgctgctg atgcccggac ccgataacgc cgggggcagt aaggagatgg
     5341 tcgagggttt tcgcgcagcc attgccggaa gcccggtgcg tattgttgat attgcgcttg
     5401 gtgataacga tattgaaatc cagcgcaacc tgttgcagga gatgctggaa cgccacccag
     5461 aaatcgacgt cgttgccgga acggccattg cggcagaggc ggcaatgggt gaagggcgta
     5521 acctgaaaac gccgcttacc gtggtgtcgt tttatctttc acatcaggtg tatcgcgggc
     5581 tgaagcgggg aagagtgatt atggctgcca gcgatcaaat ggtctggcag ggggaactgg
     5641 cagttgagca ggccatcagg caattacagg ggcaatcggt gtctgataat gtcagcccac
     5701 cgattttagt tctgacgccg aaaaatgccg accgagaaca tatccgccgc tcgctgtcac
     5761 cggggggatt tcgtccggtc tatttttatc agcacacatc agcggctaag aaataacctt
     5821 caccatgttg cgtcaccagt aaatccgcgc tgagtttatg acgtaaacga cgaattaaca
     5881 catcgacggt gcgcaggtca gggttttcca cccgacgcgc agaaagcata cgcagcagac
     5941 gctcacggct gagaatttcg cccggattag tcacaaatgc caccaacatt tcatactctg
     6001 cgcgggtcag tttaatcggc tcgccatccc gctccagcgt atggcgcgac acattcaggc
     6061 aataacccgc aaagcgatag cagtagtcct gagtgtgcgg ttgagcttgt cgcgcgaggt
     6121 cgattcgcca gagcagattt ttcacccgta ctaccagttc gcgcagttcc agcggtttgg
     6181 tgacgtaatc gtctgcgccc atttccagcc caacaatacg gtcaatccga tcgctgcgtc
     6241 cggtaaccag aataatcccc accgttgagc gttctcgcag ggcgcgggtt aacatcaggc
     6301 cattttcatc gggtaagttg atatccagca gaattaaatc taccggctga ttctgcataa
     6361 tttcccgtag cccggcaccg ctcgccgtaa cggaaacggt atacccctcc tgagtgaagt
     6421 aggattgtaa tcgcgcctgg gtaaccggct tatcttcaac aataacaatg tgatgtggca
     6481 tcagagggtt ttactcattc tgttcatatc tgttcatatt tgctcattaa gatcgcttca
     6541 ctaaaccata attctacagg ggttattatg cggaaactct ggaacgcgct acgccgaccc
     6601 agtgctcgtt ggtcggtact ggcgctggtc gcaattggga ttgtgattgg cattgcgctg
     6661 attgtattgc cacacgttgg gatcaaagtc accagcacaa ccgaattttg tgtcagttgc
     6721 cacagtatgc aaccggtgta tgaagaatat aaacagtcgg tgcatttcca gaacgcctcc
     6781 ggcgtgcgag ctgaatgcca tgactgtcat atcccgccgg atattccagg catggtgaag
     6841 cgcaaactgg aagcgagcaa cgacatttac cagaccttta ttgcccactc cattgataca
     6901 cctgaaaaat tcgaagccaa acgcgcggaa cttgccgagc gtgaatgggc gcgaatgaaa
     6961 gaaaacaact cggcaacctg tcgctcctgc cataactacg atgcgatgga tcatgccagg
     7021 cagcatcctg aagcggcacg tcagatgaag gtggcagcga aagataatca atcctgcatc
     7081 gactgtcata aaggtattgc ccaccagtta ccggatatga gtagcggctt ccgtaagcag
     7141 ttcgatgagc tgcgcgccag tgctaatgac agtggtgaca cgctgtactc tattgatatt
     7201 aagccaattt atgcggcgaa aggcgataaa gaagcctctg gttctctgct gcctgcttcg
     7261 gaagtgaaag tccttaaacg tgacggcgac tggctgcaaa ttgaaattac cggctggacg
     7321 gaaagcgccg gacgtcagcg tgtactcacc caattcccag gtaaacgcat ctttgttgcc
     7381 tcgattcgtg gtgatgtgca gcagcaggtg aaaacgctgg agaaaaccac cgttgccgac
     7441 accaataccg agtggagcaa gttgcaggcc accgcgtgga tgaagaaagg cgacatggtg
     7501 aacgatatca aaccgatctg ggcctatgcg gattcgttgt acaacggcac ctgtaacgag
     7561 tgccacggcg caccggaaat cgcccacttt gacgctaacg gttggatcgg cacgctcaac
     7621 ggcatgattg gctttaccag tctcgataaa cgtgaagaac gcactttgtt gaaatatctg
     7681 caaatgaatg cgtctgacac cgcaggtaag gctcacggcg ataagaagga agaaaaataa
     7741 tgaacaataa cgatctcttt caggcatcac gtcggcgttt tctggcacaa ctcggcggct
     7801 taaccgtcgc cgggatgctg gggccgtcat tgttaacgcc gcgccgtgca actgcggcgc
     7861 aagcggcgac tgaggctgtc atctcgaaag agggcattct taccgggtcg cactgggggg
     7921 ctatccgcgc gatggtgaag gatggtcgct ttgtggcggc aaaaccgttc gaactggata
     7981 aatatccgtc gaaaatgatc gccggattgc cggatcacgt acacaacgcg gcacgtattc
     8041 gttatccgat ggtacgcgtg gactggctgc gtaagcgcca tctgagcgac acctcccagc
     8101 gcggtgataa ccgttttgtg cgcgtgagct gggatgaagc cctcgacatg ttctatgaag
     8161 aactggaacg cgtacagaaa actcacgggc cgagtgcctt gctgaccgcc agtggttggc
     8221 aatcgacggg gatgttccat aacgcttcgg ggatgctggc gaaagctatt gccttgcatg
     8281 gtaatagcgt tggtacgggc ggagattact ctaccggtgc tgcgcaggtg atcctgccgc
     8341 gcgtagtcgg ttcaatggaa gtgtatgaac agcaaacctc ctggccgctg gtattgcaga
     8401 acagcaaaac cattgtgctg tggggctccg atttgctgaa aaaccagcaa gcgaactggt
     8461 ggtgcccgga tcacgatgtt tatgaatatt acgagcagct aaaagcgaaa gtcgccgccg
     8521 gtgaagttga ggtcatcagc atcgatccgg ttgtcacatc cacccatgag tatctggggc
     8581 gcgagcatgt gaagcacatt gcggttaacc cgcaaactga cgtgccgctg caactggcgc
     8641 tggcacatac gctgtacagt gaaaacctgt acgacaaaaa cttcctcgct aactactgtg
     8701 tgggttttga gcagttcctg ccgtatctgc tgggtgagaa agacggtcag ccgaaagatg
     8761 ccgcatgggc tgaaaaactg accggcattg atgccgaaac cattcgtggg ctggcgcggc
     8821 agatggcggc gaacagaacg caaattattg ctggctggtg cgtgcagcgt atgcagcacg
     8881 gtgaacagtg ggcgtggatg attgtggttc tggcggcgat gctggggcaa attggcctgc
     8941 caggtggtgg ttttggctgg cactacaacg gcgcaggcac gccggggcgt aaaggcgtta
     9001 ttctgagtgg tttctccggc tctacgtcga ttccgcctgt tcacgacaac agtgactaca
     9061 aaggctacag cagcactatt ccgattgccc gttttatcga tgcgatcctc gaaccgggga
     9121 aagtgatcaa ctggaacggt aaatcggtaa aactgccgcc gctgaaaatg tgtatttttg
     9181 ccggaactaa cccattccat cgccatcagc agatcaaccg cattattgaa ggctggcgca
     9241 agctggaaac ggttatcgcc atagataacc agtggacctc aacctgccgc tttgccgata
     9301 tcgtgctgcc tgcgaccacg cagtttgagc gtaacgatct cgaccagtac ggcaaccact
     9361 ccaaccgtgg cattatcgcc atgaaacagg tggtgccgcc gtagttcgag gcgcgcaacg
     9421 actttgatat tttccgcgag ctgtgccgtc gctttaatcg tgaagaagcc tttaccgaag
     9481 ggctggacga aatgggctgg ctgaaacgca tctggcagga aggtgtacaa caaggcaaag
     9541 gacgcggcgt tcatctgcca gcgtttgatg acttctggaa taacaaagag tacgtcgagt
     9601 ttgaccatcc gcagatgttt gttcgccacc aggcattccg cgaagatccg gatctcgaac
     9661 cgctgggcac gccgagtggc ctgattgaga tctactcgaa aaccatcgcc gatatgaact
     9721 acgacgattg tcaggggcat ccgatgtggt ttgagaaaat cgaacgctcc cacggtgggc
     9781 ccggctcgca gaagtatccg ttgcatctgc aatctgtgca tccggatttc cgacttcact
     9841 cgcagctatg tgagtcggaa acgctgcgtc agcaatatac ggtagcgggt aaagagccag
     9901 tgttcattaa cccgcaggat gccagcgcgc gcggtattcg taacggtgat gtggtacgcg
     9961 tctttaacgc tcgcggtcag gtgttggcag gagcagtagt ttctgaccgc tatgcacccg
    10021 gcgtggcgcg aattcacgaa ggggcatggt acgatccaga taaaggcggc gagcctggtg
    10081 cgctgtgcaa atacggtaac cccaacgtgc tgaccatcga catcgggact tcgcagctgg
    10141 cgcaggcgac cagtgcgcac actacgctgg tggaaattga gaagtacaat ggaacagtgg
    10201 agcaggtgac ggcgtttaac ggccccgtgg agatggtggc gcagtgcgaa tatgttcccg
    10261 cgtcgcaggt gaaatcatga ccacgctgac agcacaacag attgcctgtg tttacgcctg
    10321 gctggcgcag ttgttctccc gtgaactgga cgatgaacaa ctgacgcaaa tcgccagtgc
    10381 gcagatggct gaatggtttt cgttgctgaa aagcgaaccg ccgctcactg cggcggtgaa
    10441 tgatctggaa aacagtattg ccac
//
LOCUS       DAJTSI010000135        10428 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000135 DAJTSI010000000
VERSION     DAJTSI010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10428
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.135"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            132..575
                     /locus_tag="OO327_003187"
     CDS             132..575
                     /locus_tag="OO327_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6019624.1"
                     /translation="MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            811..1629
                     /gene="ybhA"
                     /locus_tag="OO327_003188"
     CDS             811..1629
                     /gene="ybhA"
                     /locus_tag="OO327_003188"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCR6019625.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1630..2688)
                     /gene="modC"
                     /locus_tag="OO327_003189"
     CDS             complement(1630..2688)
                     /gene="modC"
                     /locus_tag="OO327_003189"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR6019626.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2691..3380)
                     /gene="modB"
                     /locus_tag="OO327_003190"
     CDS             complement(2691..3380)
                     /gene="modB"
                     /locus_tag="OO327_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR6019627.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3380..4153)
                     /gene="modA"
                     /locus_tag="OO327_003191"
     CDS             complement(3380..4153)
                     /gene="modA"
                     /locus_tag="OO327_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6019628.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     TTQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4320..4469)
                     /gene="acrZ"
                     /locus_tag="OO327_003192"
     CDS             complement(4320..4469)
                     /gene="acrZ"
                     /locus_tag="OO327_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR6019629.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4598..5386
                     /gene="modE"
                     /locus_tag="OO327_003193"
     CDS             4598..5386
                     /gene="modE"
                     /locus_tag="OO327_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR6019630.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5454..6926
                     /gene="modF"
                     /locus_tag="OO327_003194"
     CDS             5454..6926
                     /gene="modF"
                     /locus_tag="OO327_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR6019631.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7187..8203
                     /gene="galE"
                     /locus_tag="OO327_003195"
     CDS             7187..8203
                     /gene="galE"
                     /locus_tag="OO327_003195"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR6019632.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8213..9259
                     /gene="galT"
                     /locus_tag="OO327_003196"
     CDS             8213..9259
                     /gene="galT"
                     /locus_tag="OO327_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR6019633.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9263..10411
                     /gene="galK"
                     /locus_tag="OO327_003197"
     CDS             9263..10411
                     /gene="galK"
                     /locus_tag="OO327_003197"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR6019634.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
BASE COUNT         2672 a         2753 c         2621 g         2382 t
ORIGIN      
        1 ttgccgaaga agatccagct tctgcaacca ggccattcag aactgaagtg aagcgcagtc
       61 gactgttaat tgacgagtat ctgttaatca ggaaaatagc agacgtccag aacgaatggt
      121 tcggcctttg catggatctt gcactggtaa caggacagcg caaaggagat ctggcggcta
      181 tgagatggga ggatataaga gatagcaggc tatacgtgga acaacaaaaa acaggggcaa
      241 aaattcgcat ttctctcccc accacgattt ccagattgaa tttaacactt gccgatgtac
      301 tggataactt gaaaaaaata aacggtaaaa acgaaaagtt actcggaggg aagacagcca
      361 ggacaattgc agcacagttc aggattgccc gagacacatc cggattgaaa tgggaaggtg
      421 acccaccgcc atttcatgaa atccgaagtt tatccggtcg cctgcactca gcagaaaaag
      481 gcagtgattt cactcaggcg ctccttggac atcgttcatc cagcatgacg gataaatacc
      541 gtgacggacg agggagggaa tggaaagaca tttagaatta atttcgaatg atttcgaaat
      601 gccacttgta attaattgat tataaagaat taattcctaa agagcatttg cttcatgaat
      661 gctcctttgc atttagcaac tgactgtaac agctaaaatt agtcgctttt ggcggtgaat
      721 gctgaattaa aacgcagaat tttgtccgat agtggtatcg gtgtaccatt cgcgcagacg
      781 aaaattctta accttaatct ggaatacgcc atgaccacac gcgtgattgc tctcgactta
      841 gacggcacct tattgacccc gaaaaagacc ttgcttccat catcgataga agccctggcc
      901 cgcgctcgcg aagcaggcta tcaattaatc atcgtcacag gtcgccatca cgtcgctatt
      961 catccttttt atcaggcgct ggcgctggat acacctgcta tttgctgtaa tggcacctat
     1021 ttgtatgatt atcatgcaaa aaccgtgctg gaagcggacc caatgcccgt taataaagcc
     1081 ctgcaactca ttgagatgct gaatgaacac cacattcacg gtctgatgta tgtcgatgat
     1141 gcaatggtct atgagcaccc gaccgggcat gtcattcgca cgtctaactg ggcgcaaacc
     1201 ctgccgccgg aacagcgtcc gactttcaca caagtcgctt ctctggctga aacgacgcaa
     1261 caagttaacg ccgtatggaa gttcgccctg acgcacgatg acctgccgca attgcagcat
     1321 tttggtaagc atgtcgaaca tgaactggga ctggagtgtg aatggtcctg gcacgatcag
     1381 gttgatattg cacacggcgg caacagcaaa ggtaaacgtt tgacgaaatg ggttgaggcg
     1441 caaggttggt cgatggaaaa cgtcgtggca ttcggcgata actttaatga tatcagtatg
     1501 ctggaagccg ctggtacagg cgtggcgatg ggcaacgccg atgacgcggt aaaagcgcgc
     1561 gccaacattg tgattggtga taacaccacc gacagcattg cccagttcat ttatagccac
     1621 ctgatttaat caggcggtta tcgacacact tttaatttgc gcgtacagcc acaggccagg
     1681 tttgatcgcc agttcatccc tggcccacgg gctgatacgc gcccacagcg ttttaccgcc
     1741 gacttccagc tccacttcca cctggccgtt gtcgtcataa ctattaacaa cttttgcccg
     1801 caatacgtta cgaatgctgg tttgctgcgg cggttgtaaa accagagaaa catcggaagc
     1861 ctgaatgcgg atacgtagcg cagcttgcag cggttcgccc agcttattga cccacaaatg
     1921 ctgatcgccc agcgccagcg cggtcatcgc gtaatgcgga tgatgctcaa gcaccgtcac
     1981 tttcagaatg ctactttgtt gctctttcgg cagccacgga ttcatcacgc tactgcccca
     2041 cacttcctcc agcgcgccaa aggctttcac ctgaccgttt tccagtacca tcactctgtc
     2101 tgccagatgg aggatctcat ccagcgaatg gctgacatac aacatcggaa tgttgatttc
     2161 ccgtgtcagc cgttgcagat aaggcaacag ttcgcgttta cgcggaatat ccagtgacgc
     2221 cagcggttca tccagcaaca gcaattccgg tgctgtcagc aaagcccgac caatcgccac
     2281 gcgctgtttt tcgcctccgg acaggctgcc tggtaaacgg tcaagcaacg gttcaatgcc
     2341 taaaagcgcc accagcttat cgaactgatc gaccatactt ttcgacatgc cgtagcgcag
     2401 attgccacgc actttgtaat gcgggaacaa ccgcgcatcc tgaaaaacat agccaacacg
     2461 acgcttttcc ggcgtcaggc agataccttt ttcggcatca tttagtaccc gcccattgag
     2521 gacaatccgc cctttttgcg ggcgcgtcag tccactgatg gcgttaatca gcgaagtttt
     2581 tccggcaccg gagacgccaa agatagcagt gatgccattg gcgggcagcg tttcattaat
     2641 ggtcaggcaa tggttgccca acgtctggga aaaattcagc tccagcatga ttagcgcccc
     2701 gcccgttcac ggctgattct ggccagccat tctgaaatca acagggagat catcgccagc
     2761 gcaatagaaa taatgcacag tctcgccgcg ccactttccc cgccgggggt ctggatcagg
     2821 gtatacatgg cagaaggaat ggttcgcgtt tcgctaggaa tgttcgacac aaaggtgatg
     2881 gttgcaccaa actcaccgag agaacgagca aaagccagta ccgtaccaac aataattccc
     2941 ggtaaggtca gcggtaacgt gatagtaaag aaaacgcgcc agcgcccggc acctagtgtt
     3001 cttgcggcct gttccagttt gacatcaacc ccttccagcg ccagacgaat tgcccgcacc
     3061 atcagcggaa acgacatgac ggcggcagcg agaaccgcgc cgcgccagct aaaggcgaag
     3121 gtaataccaa accagtcata cagacgttca ccgataaatc cgcgccgtcc catcgaaact
     3181 aataataagt aaccgacgac cacgggcggt aacaccagcg gtagatgcag tacgctgtcg
     3241 agcagagctt tgcccggaaa cgtgcaacgc accagtaacc aggcaaaaaa gatcccaaac
     3301 ggcaggctaa acagcacagc cagggaagaa actttcaggc ttaataaaac tgcctgccat
     3361 tctggatcgg tcagtatcat tacttggttg taaatccgta acgtttaaag atttccgctg
     3421 cctgcggtcc cttcagataa tcataaaatg ctttcactgt cgcattgtta tgcccttcca
     3481 caacagcaac cggatattcc acttttttat gtgaatcttc cgggaaggtg gcaaccactt
     3541 ttaccccttt gctggcaact gcgtcagaac cgtagacaat gcccagaggc gcttcgttac
     3601 gttcgaccag cgccagcgcc ccacgaacat cttccgctgg ggccagtttc ggagagagcg
     3661 tatcccatgc gcccagtttt tgcagtgctt cttttgcata aatgccagcg ggaacatgtt
     3721 ccggatcgcc aaccgccagg cgaccgccat tcagcagcga agtccagtta gttttgctgt
     3781 cgatggtgaa atctttctgc tcgctggctt tcggtgctac aacgaccagg ctattgccga
     3841 gcagtgtctg acgcgtagca gtatcgatcg cttttttatc aaccgcataa tccatccatt
     3901 tctgatcggc agaaataaac agatccgcag gggcacccgc ttcaatctga cgggcgagag
     3961 tagatgacga agcgaaagaa gaaaccacat ccacgccttt ctctttttta tactgcgtag
     4021 taatgtcctg cattgcgtta gtcagtgatg cggcggcgaa caccgtgatt ttcccttcat
     4081 ctgccagtgc attgccagca actgcgaaag agagtgccgc cccggcaaac aagttcaacc
     4141 atttacgagc cattggtaac cccttaatgt aacgttatgt aggcgacaat ataacgacta
     4201 actcggaatt ttcccagcag ttattgctaa cctttgtgag gtagataaga aaaaatatcg
     4261 gcaggaaaag caggaagttg agaaaaagaa aatgcccgac taagcgggca ctcagggaat
     4321 caatgatttt gtccgggctg gtctttttta ccaacaccag aaaagatgtt gaatacttca
     4381 ccaagaccgt aaatcagacc caggatgatg gccatcacga caggtaccat gattacggcg
     4441 aataccagac tttttaataa ctctaacatg gtcaactcca gatatagtca tgagactatt
     4501 ctaaccgcta agcacagaaa agcactcccc ttttgtgcgg tcagctttgc gtggcgttcg
     4561 ttttccgtca caataagact tttgccagga cattgttatg caggccgaaa tccttctcac
     4621 ccttaagctc caacaaaaat tattcgccga cccgcgccgc atttcgctac taaaacacat
     4681 tgcgctttcc ggttccatta gccagggagc gaaagatgcc ggtatcagct ataaaagcgc
     4741 ctgggatgcc attaacgaga tgaatcagtt aagtgagcat attctggtcg agcgcgcaac
     4801 aggcggtaaa ggtggcggag gcgcagtact gacccgctat ggtcagcgac tgattcagct
     4861 ctatgactta ctggcgcaaa tccagcaaaa agcctttgat gtgttaagtg acgatgacgc
     4921 cctgccgctg aacagcctgc tggccgcgat ctcacgtttt tcactgcaaa ccagcgcccg
     4981 taaccagtgg ttcggtacca tcaccgcccg cgatcatgat gacgttcaac agcatgttga
     5041 tgtcttactg gctgacggaa aaacacgcct gaaagtcgca attaccgcac aaagcggcgc
     5101 acgtctgggg ctggatgaag gcaaagaagt gttgatattg ctaaaagcgc cgtgggtagg
     5161 cattactcag gacgaagcgg tcgcacaaaa cgccgacaac caattaccgg gtattattag
     5221 tcatattgag cgcggcgcag agcagtgcga agtattaatg gcgctacccg acgggcaaac
     5281 actgtgcgcc acagtgccgg taaatgaagc gacttctctt cagcaagggc agaatgtcac
     5341 ggcctacttt aatgccgaca gcgtgattat cgccacgctg tgctaagcgt gttgacaatt
     5401 tgttatgaaa cacgtatccc tgtcagtaat cgctgcacaa aatgggatat aaaatgtcat
     5461 cgttgcaaat tttgcaaggc acgtttcgtc ttagcgacac aaaaacgctg caattgcctc
     5521 agctaacgtt aaacgcgggt gatagttggg cgtttgtcgg ttcgaatgga agtggaaagt
     5581 cggcgctggc ccgcgcgctg gcgggggaac ttccgctttt gaaaggtgaa cggcaaagcc
     5641 agttttccca catcactcgt ctctccttcg agcaattgca aaagctcgtc agcgacgaat
     5701 ggcaacggaa taacaccgat atgctcggcc ctagcgaaga ttacaccgga cgcactacgg
     5761 ctgagattat tcaggatgaa gtaaaggatg caccgcgttg catgcaactg gcgcagcagt
     5821 tcggtattac cgccctcctc gaccgacgct ttaaatacct ttccactggc gagacgcgaa
     5881 aaaccctgct gtgtcaggcg ctgatgtcgg agcctgactt gttgattctt gatgagccgt
     5941 tcgatggcct ggatgttgcc tcacgtcagc agctggctga gcgactcgcc tcgttacatc
     6001 agtccggtat tactttggta ctggtgctca atcgcttcga taagatcccg gaatttgtcc
     6061 agtttgctgg cgtgctggcg gattgtacgt tagcggaaac tggcgctaaa gaggaactgc
     6121 tccagcaagc actcgtcgcg caacttgcgc atagtgaaca gcttgaaggt gtgcaactgc
     6181 cggagccgga tgaaccttca gcacgtcacg ccttacccgc caacgaatcg cgcattgtgc
     6241 tgaacaatgg cgtggtttct tataatgatc gccccattct taataacctt agctggcagg
     6301 tgaatccagg cgaacactgg caaattgtcg ggccaaatgg cgcgggaaaa tcgacgttat
     6361 taagcctgat tactggcgat catccgcaag gttacagcaa cgatttgacg cttttcggcc
     6421 gacgtcgcgg cagcagcgaa accatctggg acatcaaaaa gcatatcggt tacgtcagca
     6481 gtagtttgca tctggattac cgggtcagca ctaccgtgcg taatgtgatt ctttctggct
     6541 attttgattc gattggcatt tatcaggtcg tttcggatcg ccagcaaaaa ctggtgcagc
     6601 agtggctgga tattctcggc attgataaac acacggctga cgctccgttc catagtcttt
     6661 cctggggaca gcagcgtttg gcgctgatcg tccgcgcact ggtgaaacat ccgacgttgc
     6721 ttattctcga tgaaccacta caggggcttg atccgctcaa tcgccaactt atccgccgtt
     6781 ttgttgatgt gctgattagc gaaggtgaaa cgcaattgtt gtttgtttcg caccacgctg
     6841 aagatgcgcc tgcctgtatt acccatcgcc ttgagttcgt gccggacggt ggactctatc
     6901 gctatgtgct gacaaaaata tattgagtcg gtagtgctga ccttgccgga ggcggcctaa
     6961 gcgcactctc cggccaacgg ttcgacgcat gcaggcatga aaccgcttct tttttcagat
     7021 aaaaagcgca atcattcata aaccctctgt tttataatca cttaatcgcg cataaaaaac
     7081 ggctaaattc ttgtgtaaac gattccacta atttattcca tgtcacactt ttcgcatctt
     7141 tgttatgcta tggttatttc ataccataag cctaatggag cgaattatga gagttctggt
     7201 taccggtggt agcggttaca ttggaagtca tacctgcgtg caattactgc aaaacggtca
     7261 tgatgtcatc attcttgata acctctgtaa cagtaagcgc agcgtactgc ctgttatcga
     7321 gcgtttaggc ggcaaacatc caacgtttgt tgaaggcgat attcgtaacg aagcgttgat
     7381 gaccgagatc ctgcacgatc acgctatcga caccgtgatc cacttcgccg ggctgaaagc
     7441 cgtcgccgaa tcggtacaaa aaccgctgga atattacgac aacaatgtca acggcactct
     7501 gcgcctgatt agcgccatgc gcgccgctaa cgtcaaaaac tttattttta gctcctccgc
     7561 caccgtttat ggcgatcagc cccaaattcc atacgttgaa agcttcccga ccggcacacc
     7621 gcaaagccct tatggcaaaa gcaaattgat ggtggaacag atcctcaccg acctgcaaaa
     7681 agcccagccg gactggagca ttgccctgct gcgctacttc aacccggttg gcgcgcatcc
     7741 gtcgggcgat atgggcgaag atccgcaagg cattccgaat aacctgatgc catacatcgc
     7801 ccaagttgct gtaggccgtc gcgactcgct ggcgattttt ggtaacgatt atccgaccga
     7861 agatggtact ggcgtacgcg attacatcca cgtaatggat ctggcggacg gtcacgtcgt
     7921 ggcgatggaa aaactggcga acaagccagg cgtacacatc tacaacctcg gtgctggcgt
     7981 aggcagcagc gtgctggacg tggttaatgc cttcagcaaa gcctgcggca aaccggttaa
     8041 ctatcatttt gcaccgcgtc gcgaaggcga ccttccggcc tactgggcgg acgccagcaa
     8101 agccgaccgt gaactgaact ggcgcgtaac gcgcacactc gatgaaatgg cgcaggacac
     8161 ctggcactgg cagtcacgcc atccacaggg atatcccgat taaggaacga ccatgacgca
     8221 atttaatccc gttgatcatc cacatcgccg ctacaacccg ctcaccgggc aatggattct
     8281 ggtttcaccg caccgcgcta agcgcccctg gcagggggcg caggaaacgc cagccaaaca
     8341 ggtgttacct gcgcacgatc cagattgctt cctctgcgca ggtaatgtgc gggtgacagg
     8401 cgataaaaac cccgattaca ccgggactta cgttttcact aatgactttg cggccttgat
     8461 gtctgacacg ccagatgcgc cagaaagcaa cgatcctttg atgcgttgcc agagcgcgcg
     8521 cggtaccagc cgggtgatct gcttttcacc ggatcacagt aaaacgttgc cagaactgag
     8581 cgttgcggca ttgacggaaa tcgtcaaaac ctggcaggag caaaccgcag agctgggaaa
     8641 aacgtacccg tgggtgcagg tctttgaaaa caaaggtgcg gcgatgggct gctctaaccc
     8701 gcatccgcac ggacagattt gggcaaatag cttcctgcct aacgaagctg agtgcgaaga
     8761 ccgcctgcaa aaagaatatt ttgccgaaca gaaatcacca atgctggtgg attatgttca
     8821 gcgcgagctg gcagacggta gccgtaccgt tgtcgaaacc gaacactggt tagccgttgt
     8881 gccttactgg gctgcctggc cgttcgaaac gctactgctg cccaaagccc acgttttgcg
     8941 gatcaccgat ttgaccgacg cccagcgcag cgatttggcg ctggcgttga aaaagctgac
     9001 cagtcgttat gacaacctct tccagtgctc cttcccctac tctatgggct ggcacggcgc
     9061 accatttaat ggcgaagaga atcaacactg gcagctgcac gcgcactttt atccgcctct
     9121 gctgcgctcc gccaccgtac gtaaatttat ggttggttat gaaatgctgg cagagaccca
     9181 gcgagacctg accgcagaac aggcagcaga gcgtttgcgc gcagtcagcg atatccattt
     9241 tcgcgaatcc ggagtgtaag aaatgagtct gaaagaaaaa acacaatctc tgtttgccaa
     9301 cgcatttggc taccctgcca ctcacaccat tcaggcgcct ggccgcgtga atttgattgg
     9361 tgaacacacc gactacaacg acggtttcgt tctgccctgc gcgattgatt atcaaaccgt
     9421 gatcagctgt gcaccacgcg atgaccgtaa agttcgcgtg atggcagccg attatgaaaa
     9481 tcagctcgac gagttttccc tcaatgcgcc cattgtcgcg catgaaaact atcaatgggc
     9541 gaactacgtt cgtggcgtgg tgaaacatct gcaactgcgt aacaacagct tcggcggtgt
     9601 ggacatggtg atcagcggca atgtgccgca gggtgccggg ttaagttctt ccgcttcact
     9661 ggaagtcgcg gtcggaaccg tattgcagca gctttatcat ctgccgctgg acggcgcaca
     9721 aatcgcgctt aacggtcagg aagcagaaaa ccagtttgtt ggctgtaact gcgggatcat
     9781 ggatcagcta atttccgcac tcggcaagaa agatcatgcc ttgctgattg actgccgctc
     9841 actggggact aaagcagttt ccatgccgaa aggtgtggct gtcgtcatca tcaacagtaa
     9901 cttcaaacgt accctggttg gcagcgaata caacacccgt cgtgaacagt gcgaaaccgg
     9961 tgcgcgtttc ttccagcagc cagctctgcg cgatgtcacc attgaagagt tcaacgctgt
    10021 tgcacatgag ctggacccaa tcgtggcgaa acgcgtgcgt catatcctga ctgaaaacgc
    10081 ccgcaccgtt gaagctgcca gcgcgctgga gcaaggcgac ctgaaacgta tgggcgagtt
    10141 gatggccgag tctcatgcct ctatgcgcga tgatttcgaa atcaccgtgc cgcaaattga
    10201 cactctggta gaaatcgtca aagctgtgat tggcgacaaa ggtggcgtac gcatgaccgg
    10261 cggcggattt ggcggctgta tcgtcgcgct gatcccggaa gagctggtgc ctgccgtaca
    10321 gcaagctgtc gctgaacaat atgaagcaaa aacaggtatt aaagagactt tttacgtttg
    10381 taaaccatca caaggagcag gacagtgctg aacgaaactc ccgcactg
//
LOCUS       DAJTSI010000136        10305 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000136 DAJTSI010000000
VERSION     DAJTSI010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10305
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.136"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            21..770
                     /gene="yjfP"
                     /locus_tag="OO327_003198"
     CDS             21..770
                     /gene="yjfP"
                     /locus_tag="OO327_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6019635.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(767..1522)
                     /gene="ulaR"
                     /locus_tag="OO327_003199"
     CDS             complement(767..1522)
                     /gene="ulaR"
                     /locus_tag="OO327_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR6019636.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(1630..2694)
                     /gene="ulaG"
                     /locus_tag="OO327_003200"
     CDS             complement(1630..2694)
                     /gene="ulaG"
                     /locus_tag="OO327_003200"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR6019637.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            3049..4446
                     /gene="ulaA"
                     /locus_tag="OO327_003201"
     CDS             3049..4446
                     /gene="ulaA"
                     /locus_tag="OO327_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR6019638.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            4462..4767
                     /gene="ulaB"
                     /locus_tag="OO327_003202"
     CDS             4462..4767
                     /gene="ulaB"
                     /locus_tag="OO327_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR6019639.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            4777..5241
                     /gene="ulaC"
                     /locus_tag="OO327_003203"
     CDS             4777..5241
                     /gene="ulaC"
                     /locus_tag="OO327_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR6019640.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            5255..5905
                     /gene="ulaD"
                     /locus_tag="OO327_003204"
     CDS             5255..5905
                     /gene="ulaD"
                     /locus_tag="OO327_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR6019641.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            5915..6769
                     /gene="ulaE"
                     /locus_tag="OO327_003205"
     CDS             5915..6769
                     /gene="ulaE"
                     /locus_tag="OO327_003205"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR6019642.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKACDWVKARMA
                     KAGMVEAA"
     gene            6769..7455
                     /gene="ulaF"
                     /locus_tag="OO327_003206"
     CDS             6769..7455
                     /gene="ulaF"
                     /locus_tag="OO327_003206"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR6019643.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(7584..7859)
                     /locus_tag="OO327_003207"
     CDS             complement(7584..7859)
                     /locus_tag="OO327_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6019644.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            8186..8581
                     /gene="rpsF"
                     /locus_tag="OO327_003208"
     CDS             8186..8581
                     /gene="rpsF"
                     /locus_tag="OO327_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR6019645.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            8588..8902
                     /gene="priB"
                     /locus_tag="OO327_003209"
     CDS             8588..8902
                     /gene="priB"
                     /locus_tag="OO327_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR6019646.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            8907..9134
                     /gene="rpsR"
                     /locus_tag="OO327_003210"
     CDS             8907..9134
                     /gene="rpsR"
                     /locus_tag="OO327_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR6019647.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            9176..9625
                     /gene="rplI"
                     /locus_tag="OO327_003211"
     CDS             9176..9625
                     /gene="rplI"
                     /locus_tag="OO327_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR6019648.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(9696..10259)
                     /locus_tag="OO327_003212"
                     /pseudo
     CDS             complement(9696..10259)
                     /locus_tag="OO327_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
BASE COUNT         2437 a         2546 c         2736 g         2586 t
ORIGIN      
        1 cagacaaaaa ggagagacag atgattgaaa tagaatcacg cgagctggca gatattcccg
       61 ttcttcatgc ttatcctgtc gggcaaaaag ataccccgtt accgtgcgta attttttatc
      121 acggctttac ttcatccagc ctggtgtata gctattttgc cgttgcgctg gcgcaggctg
      181 gtttgcgggt gatcatgccg gatgcgcccg atcacggtag ccgttttagt ggcgacgaag
      241 cgcggcggtt aaatcaattc tggcaaatct tgctacaaag tatgcaggaa ttcactactt
      301 tacgtgcggc aatagccgaa gaaaactggc tgcttgatga ccgtctggca gtcggtggcg
      361 cgtcgatggg cgcgatgacg gcactgggaa ttaccgctcg ccaccccacg gtgaaatgta
      421 ccgccagcat gatgggatcg ggctatttta catcgctcgc ccgttcactg tttccaccgc
      481 tgatacctga aacggcagca cagcagaatg aattcaataa aattgtcgcg ccgctggcag
      541 agtgggaagc gacaaaccac ctggaacaac ttggggacag acctctactg ctgtggcatg
      601 gcctcgacga tgacgttgtg cctgccgacg aatcactacg tttgcagcag gccttaagcg
      661 agacgggacg ggataaactg ctaacctgtt catggcagcc aggcgtgcgt caccgcatta
      721 cgcctgaggc gttagatgct gccgtgacat ttttccgcca gcatctttaa acacgcagaa
      781 tgctgacccc ttgcgcttcc agttgttgca ggatttccgg gttagcattt ttgccggtga
      841 tcagcatatc gatttgatcg gcacggctaa aaagcattcc cgcgcgttcg ccaatcttac
      901 tgctatcaac cagtaccacc agtttcccta ccacgctcag catcttctgc tctgccattg
      961 ctgtcagcat atcggtttta tacaaccctt ctgcggtcag cccttttccg ctggtaaaca
     1021 tccagtgccc ggcatagaga ctgttttcgc tgccctgcgg gcttaaagtg atggactgac
     1081 ttttgttgta ctgtccaccc ataatgatca cgctgtcatg ttcttgatcg atcaggtaat
     1141 ttgccagcgg tagataatta gtgatgattt gcactggctt gccacacatt tcccgcccaa
     1201 gcagaaacgc ggtggagccg cagttgatga ctacgctttc gcccggatta accagctgcg
     1261 aggccgcttt agcaatacgt actttttcat cgtgattctg cgcctgatgc agattcatcg
     1321 gcgtccagcg cgggcgctgt tgggtaatag cttctgcgcc attgcgcacc tttttcagtt
     1381 tgccgctttc gccaagttta ttgatatcgc gtcgcgcagt ggcaggcgaa attcccagac
     1441 gctcaacgac tttctcaacg gtcacaaagc ccaattgtgc gagcatttcc aggaggattt
     1501 gatgtctttg tgcttcagtc atgagctatt ccgataaaat ttgatttttt tagatgatat
     1561 ttgaaatagc caggaaatac tacgccggat agcgcgaact tctccacgct atccggtata
     1621 aacgacagat tacaggaatg acttgaacgg cagatccggt tcaatagtga agcaatcatc
     1681 gaaaccgcgc ggatagtggt actcgaagtt gtctttatcc agcggccagg taaatttgcc
     1741 gccaacctgc cagataaacg gcttgaagcc atacttcaga cgatcttttt tgatctccca
     1801 cagcacgcgg atctcttgcg gatcggcctg gaagtttgac cagatgtcgt ggtggaacgg
     1861 gataaccact ttcgcattca gggcttcacc catacgcagc atatcggcgc tggtcatttt
     1921 gtcggtaata ccgcgcgggt tctcaccata cgatcctaac gccacatcga tctgatgttc
     1981 gttaccgtgc ttcgcataat agttagagta gtgggaatcg ccactgtgat atagggaacc
     2041 gccaggggtt ttgaacaggt agtttactgc gcgatcgtcc atgccatctg gcagtacgcc
     2101 agccgctttt tgatcggcag gcagggtgat cagtgcagta cggtcgaaag cgtcaagcgc
     2161 atgaatttca atgtctttca cttttacaac atcgcccggt ttgaccacga tgcaacgctc
     2221 tttcggtacg ccccagccaa tccacaaatc aacgcaggtt ttcggtccaa tgaatggtac
     2281 gtcatcagca cagttctgca tcacggcagc agcgacgtta acgtcgatat gatcgttgtg
     2341 atcgtgagtc gccagtaccg cgtcgatctg gcgaatcgca aacggatcaa gaacaaacgg
     2401 ggtggtgcgt aggtttggct gcagtttttt cacgccagcc atgcgctgca tctggtgacc
     2461 ctgtttcatt aacgggttac cgtgactttg tttgccagtg ccgcaccaga aatcaacgca
     2521 aacgttggtg ccaccttctg atttcaacca gatcccggtg cagcctagcc accacatcgc
     2581 aaatgtgcca ggggcgacct gctcttgttc aatttcttca ttcagccagc taccccactc
     2641 cgggaaagtg ctcaggatcc aggattctcg ggtgatactt ttcactttac tcatcgccat
     2701 ttaccttcat gatagttcaa ttcgaatcaa catgtgattg gttttgatta atcctgacac
     2761 tattccttca ggaaggtaat gaccattttt tgactttttg ccagggaagt tgttgttgat
     2821 tttgagtggg gaaagattta atggaatgtg tagttaaatt aactaaatga atttaaataa
     2881 ataattgttt cgttgtgtga atcccactct atccatgtgg aattatttgc gggtcgcgtc
     2941 acatttaatc ataaataatc ttgttgtgat tacttttgaa aattagagtg agtgcacaac
     3001 attccgggtg tgcggaatac ccggttacct cttcttcagg agatcgttat ggagatcctc
     3061 tacaacatct ttaccgtgtt ttttaaccag gtcatgacca atgccccgtt gttgctgggt
     3121 attgtgacct gtctgggcta catcctactg cgcaaaagtg tcagcgttat tattaaaggc
     3181 acgattaaaa ccataattgg tttcatgttg ttgcaggcag ggtccggcat cctcaccagc
     3241 accttcaaac cggtggtggc gaaaatgtcc gaagtctacg gcattaacgg cgcaatttcc
     3301 gatacttacg cgtcgatgat ggcaaccatc gaccgcatgg gcgatgccta tagctgggta
     3361 ggttacgcgg tgttgttagc gctggcgctg aacatctgtt acgtgctgct gcgtcgcatt
     3421 accggtattc gtaccatcat gctgactggt catatcatgt tccagcaggc ggggctgatt
     3481 gccgttacgt tgtttatttt cggctactcc atgtggacaa ccattatctg cacggcgatt
     3541 ctggtttcgc tctactgggg cattacctcc aacatgatgt acaagccgac tcaggaagtg
     3601 acggacggct gcggtttctc catcggtcac cagcaacagt ttgcatcatt aattgcctat
     3661 aaagttgcgc cgttcctcgg caaaaaagag gaaagcgttg aagacctcaa actgccaggc
     3721 tggctgaata ttttccacga caacatcgtc tccacggcga ttgtcatgac catcttcttt
     3781 ggtgccattc tgctctcctt cggtatcgac accgtgcagg cgatggcagg caaagtgcac
     3841 tggacggtct atatcctgca aaccggtttt tccttcgcgg tggcgatctt catcatcact
     3901 cagggtgtgc gcatgtttgt ggcggaactc tctgaagcgt ttaacggtat ctcccagcgc
     3961 ctgatccctg gtgcggttct ggcgattgac tgtgcggcta tttatagctt cgctccgaac
     4021 gccgtggtct ggggctttat gtggggcacc atcggtcagc tgattgcggt tggcatcctg
     4081 gtcgcctgcg gttcctcgat cctgattatc ccaggcttta tcccgatgtt cttctccaac
     4141 gccaccatcg gcgtgttcgc taaccacttc ggcggctggc gtgcggcgct gaagatttgt
     4201 ctggtgatgg ggatgatcga aatctttggt tgcgtctggg tggtgaaact taccggtatg
     4261 agtgcctgga tggggatggc ggactggtcg attctggcac cgccgatgat gcagggcttc
     4321 ttctccatcg gtatcgcctt tatggccgtc atcattgtaa ttgcactggc ttatatgttc
     4381 ttcgctggcc gcgcactgcg cgcagaagaa gatgcagaaa aacaattggc agaacagtct
     4441 gcttaataag gagttttgat tatgaccgta cgtattctgg ctgtgtgtgg caacggacaa
     4501 ggcagttcca tgatcatgaa gatgaaagtg gaccagtttt taacccaatc aaacattgac
     4561 cacacggtaa atagctgcgc ggttggcgaa tacaaaagcg agttgagcgg cgcggatatc
     4621 atcatcgctt ctacgcacat tgcgggcgaa atcaccgtga ccggcaacaa atacgtggtt
     4681 ggcgtgcgca acatgctctc tcctgccgac tttggcccga aactgctgga agtgatcaaa
     4741 gcgcatttcc cgcaggatgt gaagtaagga cgcgccatga aattacatga ttcgctggcg
     4801 gagaataaat ccatccgcct gcaggctgaa gctgagacat ggcaggacgc agtaaaaatc
     4861 ggcgttgacc tgctggtggc ggcggatgtt gtcgagccgc gttactacca ggcgattctg
     4921 gatgcggttg aacagcacgg tccgtacttt gtgctcgcac caggcctggc aatgccgcac
     4981 ggtcgcccgg aagagggcgt taagaaaacc ggttttgcgc tggtgacgct gaaaaagccg
     5041 ctggagttca accacgaaga taacgacccg gtggatatcc tcatcaccat ggcggcggtc
     5101 gatgccaaca cgcaccagga agtgggcatc atgcagatcg tcaacctgtt tgaagatgaa
     5161 gaaaatttcg accgtttacg tgcctgccgt accgagcagg aagtactgga tctcattgac
     5221 cgcaccaacg cggcagctta agaaggaatt gaagatgtca ttaccgatgt tgcaagtcgc
     5281 gctggacaac cagactatgg atagcgccta cgaaaccact cgcctgattg ccgaagaagt
     5341 cgacattatc gaagtgggta ccattctgtg cgtgggcgaa ggcgtgcgtg cggttcgcga
     5401 tctgaaagcg ctctacccgc acaaaatcgt gctggcagac gccaaaattg ccgatgcggg
     5461 caaaattctt tctcgtatgt gcttcgaagc caacgctgac tgggtgaccg tgatttgttg
     5521 tgcggatatc aacaccgcca aaggtgcgct ggacgtagca aaagagttta acggcgacgt
     5581 gcagatcgaa ctgaccggtt actggacctg ggaacaggcg caacagtggc gcgacgcagg
     5641 tattcagcag gtggtttatc accgcagccg tgacgcgcag gccgctggcg tggcgtgggg
     5701 tgaagccgat atcactgcga tcaaacgtct ttccgatatg ggcttcaaag tcaccgtcac
     5761 cggtggcctg gcgctggaag atctgccgct gttcaaaggc attccaattc atgtgtttat
     5821 cgccggtcgc agcatccgtg atgccgcttc tccggttgaa gccgcgcgtc agttcaaacg
     5881 ttccatcgcc gaactgtggg gctaaggagc ggatatgttg tccaaacaaa tcccgcttgg
     5941 catctatgaa aaagcgctcc ccgccgggga gtgctggctg gaacgcctgc gactggcaaa
     6001 aacgttgggc ttcgattttg tcgagatgtc ggtagatgaa actaacgagc gcctgtcgcg
     6061 ccttgactgg agccgcgaac agcgtctggc gctggtgaat gctattgttg aaaccggcgt
     6121 gcgtgtgccg tccatgtgcc tttctgccca tcgtcgtttc ccgttgggca gcgaagatga
     6181 cgcggtgcgg gcgcaggggc tggagattat gcgtaaagcc atccagttcg cccaggacgt
     6241 tggcattcgc gtgatccagc tggcgggcta tgacgtttac tatcaggaag ccaataacga
     6301 aacgcgtcgt cgtttccgtg acggcctgaa agagagcgtt gagatggcga gccgcgcgca
     6361 ggtgacgctg gcgatggaga tcatggatta tccgttgatg aactccatca gcaaggcgct
     6421 gggttacgcg cactatctca acaacccgtg gttccagctc tacccggata tcggcaacct
     6481 gtcggcgtgg gacaacgatg tgcagatgga gttgcaggcc ggaatcgggc atatcgtcgc
     6541 ggtgcatgtg aaagacacca aaccgggcgt cttcaaaaac gtgccatttg gcgaaggtgt
     6601 ggtggatttc gaacgttgct tcgaaacgct caaacagagt ggctattgcg ggccgtacct
     6661 gattgagatg tggagcgaaa cggcggaaga cccggcggca gaagtggtga aagcgtgtga
     6721 ttgggtgaaa gcgcgcatgg ccaaagcagg catggtggag gcggcataat gcaaaagcta
     6781 aaacagcagg tgtttgaagc caacatggat ctgccgcgct atgggctggt gacctttact
     6841 tggggcaacg tcagcgctat cgaccgcgaa cgcgggctgg tggtgatcaa gcccagcggc
     6901 gttgcctacg aaaccatgaa agcagacgat atggtggtgg tcgatatgag cggcaaggtg
     6961 gtggaagggg agtatcgccc atcttccgac actgcgacgc atctcgaact ctaccgtcgt
     7021 tacccgtcgc ttggcggcat tgtccatacc cactccactc atgccaccgc atgggcgcag
     7081 gcggggctgg cgatcccggc attaggcacc acgcacgctg actacttctt tggtgatatc
     7141 ccgtgtacgc gtggattaag cgaagaagag gtgcagggtg agtatgaact gaacactggc
     7201 aaagtgatta tcgaaacgct gggcaacgcc gagccgctgc atacgccggg aattgtggtg
     7261 tatcagcacg gaccgttcgc ctgggggaaa gatgcccacg acgcggtgca taacgcggta
     7321 gtgatggaag aagtggcgaa aatggcgtgg attgcgcgca gtattaatcc acaactcaat
     7381 catatcgaca gctacctgat gaataaacac ttcatgcgta agcacggtcc taacgcttat
     7441 tacgggcaga agtaaaacac gcgctgcgga aatttccttc ctcgggagat aactggtcta
     7501 attccgcagc cgtttttcaa aaaaagcccc ctgcgaaggg ggcaaagcaa actatggcaa
     7561 tgtttcgttg gttatacctg gtgctagcga taaatatccg cgctggcgtg catattgccg
     7621 ttactccccg gttcccgcat cagaattacg tggtagtacg ttgcgccttg cgcatctgtt
     7681 tcttcattta atgcctgacg tgcttcgctt tcagtggcga aattatgatt gatataaatc
     7741 acgcctaagc tttgcacatc gtccatattt ctggcctggt ggttattaat ttcaatggct
     7801 gcccatgtat ttgcacttag caaaagcaca gccagaaggg ctaaaactcg actgaacata
     7861 gatacctcct cgacggctga ctttgtgtgc tctcctctgt gatgatcttc tgatttaatt
     7921 ttaatcaatg ataaagaagt tgatggtgac catttctgat gcagttgttc aaaaaaacac
     7981 tgtgacggaa tgtgatgaac ttcaaatcag cggtttagag gttatttgcg aaaggggaga
     8041 tttatttctg ctccgccctt gagtttagcg gggtatgcca gtactataac gcgtcatttt
     8101 tcagccgacc tttaacacgt tccttgcctc cccgggattc ggctgaccca gacaggaggc
     8161 tgaataatcc gtaaggagca attcgatgcg tcattacgaa atcgttttta tggtccatcc
     8221 tgaccagagc gaacaggttc cgggcatgat cgagcgctac actgctgcca tcactggtgc
     8281 agaaggcaag atccaccgtc tggaagactg gggccgccgt cagctggctt acccgatcaa
     8341 caaactgcac aaagcacact acgttctgat gaacgttgaa gctccgcagg aagtgatcga
     8401 tgagctggaa actaccttcc gcttcaacga tgccgttatc cgcagcatgg ttatgcgtac
     8461 caagcacgct gttaccgaag catctccgat ggttaaagcg aaagacgagc gccgtgagcg
     8521 tcgcgatgat ttcgcaaacg aaaccgctga tgatgctgaa gctggggatt ctgaagagta
     8581 atttctgatg accaaccgtc tggtgttgtc cggcaccgtg tgcaggactc cccttcgaaa
     8641 ggtcagtcca tcaggaattc ctcactgcca gttcgtgctt gagcatcgtt ctgtgcagga
     8701 ggaagccggc tttcaccggc aggcatggtg tcaaatgccc gttattgtta gcggacacga
     8761 aaaccaggcc attactcaca gtataacggt cggcagtcgc ataaccgttc aggggttcat
     8821 ttcatgccac aaggcaaaga acggactgag caaaatggtt ttgcatgccg agcagattga
     8881 attgatagat tctggagact agccatatgg cacgttattt ccgtcgtcgc aagttctgcc
     8941 gtttcaccgc ggaaggcgtt caagagatcg actataaaga tatcgctacg ctgaaaaact
     9001 acatcaccga aagcggtaag attgtcccaa gccgtatcac cggtacccgt gcaaaatacc
     9061 agcgtcagct ggctcgcgct atcaaacgcg ctcgctacct gtccctgctg ccgtacactg
     9121 atcgccatca gtaatcggtc acggtccatt aatacgactt tgagaggata aggtaatgca
     9181 agttattctg cttgataaag tagcaaacct gggtagcctg ggtgatcagg taaacgttaa
     9241 agcgggctat gctcgtaact tcctggtacc gcagggtaaa gctgttccag ctaccaagaa
     9301 aaacattgaa ttcttcgaag cacgtcgcgc tgaactggaa gctaaactgg ctgaagttct
     9361 ggcagctgct aatgctcgcg ctgagaaaat caatgcactg gaaactgtta ccatcgcgtc
     9421 taaagctggc gacgaaggta aactgttcgg ttccatcggt actcgcgaca tcgctgacgc
     9481 tgtaactgca gctggcgttg aagtggctaa gagcgaagtt cgtctgccga acggcgttct
     9541 gcgtaccact ggcgaacacg aagtgagctt ccaggttcac agcgaagtat tcgcgaaagt
     9601 gatcgtaaac gtagtagctg aataattcgt tattcaacga gacgtaaaaa cgccgaccat
     9661 tggtcggcgt tttgctttct atttttcgtc aggcattaat ttcgcaagta gatccattca
     9721 ccattctctt gtgtccataa gtcaggatgg tttgctggcg ttagatgcat acgatcactc
     9781 cttatgtctc ggtcctgcgg cactcgctcg aaacctaaac ccgagtagat tgcggctgaa
     9841 tttgataagg aacgtagacc aattttcaaa tcatggggta aatcagaata cgcgtttagc
     9901 aatgcagcaa tgatgaatcc gagggcatta gaaacgcctg taacagtgtg aagttggtcg
     9961 atataaagat cgattgttgg tagttcagat gagcagcaat taggcatttg ggcaggaagg
    10021 gttacaacgt atcctatcgt ttggccggta tagtctggag tactataacc aggtcccaaa
    10081 acaacctcgt gtattgtgta tttactgttg ggttcgaagg attcgacatc ttgcaataaa
    10141 tatttcagtt ctgcggttaa cctcgcaccc gcgtaagctt tattgtattt ctgggcgtga
    10201 atattatcta atgtagcttc atggttccat ccaaaagaat tatcaccatt cattgttact
    10261 gaatcgccac gggtttaaca gacacctcag agtcatttaa gatgg
//
LOCUS       DAJTSI010000137        10237 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000137 DAJTSI010000000
VERSION     DAJTSI010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10237
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.137"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..597)
                     /gene="rspA"
                     /locus_tag="OO327_003213"
     CDS             complement(<1..597)
                     /gene="rspA"
                     /locus_tag="OO327_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCR6019649.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAV"
     gene            complement(803..1129)
                     /locus_tag="OO327_003214"
     CDS             complement(803..1129)
                     /locus_tag="OO327_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCR6019650.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1264..1605
                     /locus_tag="OO327_003215"
     CDS             1264..1605
                     /locus_tag="OO327_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCR6019651.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            1671..2200
                     /gene="speG"
                     /locus_tag="OO327_003216"
                     /pseudo
     CDS             1671..2200
                     /gene="speG"
                     /locus_tag="OO327_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2203..2913)
                     /locus_tag="OO327_003217"
     CDS             complement(2203..2913)
                     /locus_tag="OO327_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCR6019652.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3021..3326
                     /locus_tag="OO327_003218"
     CDS             3021..3326
                     /locus_tag="OO327_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCR6019653.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            3525..3845
                     /locus_tag="OO327_003219"
     CDS             3525..3845
                     /locus_tag="OO327_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005091980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase"
                     /protein_id="HCR6019654.1"
                     /translation="MSKNDRMVGISRRTLVKSTATGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVIWGSCSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQGTRLFARSLH
                     PPAY"
     gene            4405..4983
                     /locus_tag="OO327_003220"
                     /pseudo
     CDS             4405..4983
                     /locus_tag="OO327_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(5670..6659)
                     /locus_tag="OO327_003221"
     CDS             complement(5670..6659)
                     /locus_tag="OO327_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019655.1"
                     /translation="MTTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
     gene            complement(6744..7235)
                     /locus_tag="OO327_003222"
     CDS             complement(6744..7235)
                     /locus_tag="OO327_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019656.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQPYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYGRRQMNKRAEAERIAKEQRLQAE
                     RMREENMTDAEWQKELQRREQVKAERRTYGESLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
     gene            complement(7556..8056)
                     /locus_tag="OO327_003223"
     CDS             complement(7556..8056)
                     /locus_tag="OO327_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019657.1"
                     /translation="MTSDNKQEYTIHIFGQDITSQDGLFRLDEIRRVGIQIGKVEDNR
                     STEVARFMRTKAGRSLTNPENGFVKKINMGHLGTSWLADPVAAIEFGRWLDLGYGLAV
                     TRAFVSMTSSATVQSAVASAEDIKPETKEFIQRHGTGLYIAGREVSQAEYSDYQAVTA
                     GRCEWR"
     gene            complement(8046..8561)
                     /locus_tag="OO327_003224"
     CDS             complement(8046..8561)
                     /locus_tag="OO327_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019658.1"
                     /translation="MNTELPVTNRQFLIILLTNAESSVDCCRYNLDLLKLSRWYAQAE
                     DWDYEKIREMYAEPIQSYLQTHTEEELKRGLQDIFYFNNPYTCEGDSEEMDWIASRSD
                     ADLKHISNAMMQVESKWSRRLQVLTEDYENGTFDKLVPPDAERLQAKADQEQRFKSFA
                     EALAGGASYDF"
     gene            complement(8992..9360)
                     /locus_tag="OO327_003225"
     CDS             complement(8992..9360)
                     /locus_tag="OO327_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019659.1"
                     /translation="MARPIATHDNTFTKAYLQQHCGDLLSFDGQGDLSGWLDDVLTGA
                     GRLNESMASNTKPVSPYLILTQLLTHDTLTVSAVQESLSRKRVALGEPMVSTRYARYV
                     YATVVSASKSVQYHASKAGS"
     gene            complement(9692..>10237)
                     /locus_tag="OO327_003226"
     CDS             complement(9692..>10237)
                     /locus_tag="OO327_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6019660.1"
                     /translation="YQFTRHTAHTTSEARRWASLVSVVTGARSAEVCHLTKRDIVTLD
                     NGLVCIDINEDGDGKSVKNKHSVRLVPLTDGAYGFDLTSFLSWVDMQPDEGPLFGMTP
                     SAYSSWFNSRVLTEALGDSQDVSLHSLRHWLATRMKERGVNLVDAQGILGHSSQSITY
                     DLYGKGHAVGRLADVLKTALL"
BASE COUNT         2622 a         2616 c         2525 g         2474 t
ORIGIN      
        1 taccgcgtca aacaatttcg gcatgaaatc gaggtatttc tccgtcgacc acagctgctc
       61 ttccggccac tgtcctttgg ttgcgggttc ataagccaga cctttacctt tcgacatgcc
      121 gtaggtggtt ttcataccag ggattccgca ctgcacgcgg atggctttga atcccagctc
      181 ctgatgacgg gcataatcat ccagagcttc atcaatactg tgaccggtgg tatggcaata
      241 aaccatcacc ccttcacgag acgcgccgcc gagtaactgg taaagcggca tgttggcagc
      301 tttggcttta atatcccaca gcgccatatc aaccgctgaa atggccgaca tcgtaaccgg
      361 accgcgacgc cagtaagcac ctttatagaa aaactgccag atatcttcga tacggtgcgc
      421 atcgcgacca ataagccgcg gacaaagatg atcctgcaaa taagaggcca cggaaagctc
      481 acgtccattg agggtggcat ccccaaggcc cgtaataccg tcctcagtgg tgatttttaa
      541 tgtgacgaaa ttacgccccg gacaggtaac aaaaacttca gcctttacga tcttcatgtt
      601 cgattccttg catcgcttgt cgtgatgcat gaaatctacg caactgagct actgccatac
      661 aagtatgaag atcgaaaaaa gccggagtga tcacaaaaaa aggcgtatat ttgcgctgtg
      721 aatggttgac aaaagatgaa atagaatacc ttttgtcagc tgacacttcc tcgtatctta
      781 ttgataaaat ggatttatgt tcctacgtgc gcccccagcc cgcaacaatg atcaacattc
      841 cgcaaagcgc aatcaacgca cccgtccagt cataaagact tagtttcacg ccatccacaa
      901 cgcgcagcca catcaatgcc gtgcagacat aaacgccacc ataagccgcg taaacacgcc
      961 cactcgccgc aggatgcaac gttaacaacc agacaaacag cgccagtgaa atccccgccg
     1021 gaagcaacag ccagatactg gcgtttcgtt ttaaccacaa ccagggcaga aagcatccaa
     1081 taatttcaca cagcgcagta gcaaaaaata gtaacgttgt tttaatcatc tttgtcactt
     1141 attgacatca tgtatagtta tagggcgaca taatatcatc aatataaaca tcctcctggt
     1201 acgttttgcg ttcgcagtgg atggtgtaga atcaccttta atcattcata cagggaatga
     1261 attatgaaaa tcactctcag caaacgaatc ggcctgctcg cttttctgct gccttgcgca
     1321 ctggcattga gcacaactgt tcatgccgaa actaacaaac tggtgattga gtctggcgac
     1381 agtgcacaaa gccgccagca cgccgctatg gaaaaagagc aatggaatga cacgcgcaat
     1441 ctgcgccaga aagtgaataa acgcactgaa aaagagtggg ataaagccga cgccgctttt
     1501 gataaccgcg ataaatgtga gcaaagcgcc aacatcaatg cctactggga gcccaatact
     1561 ttgcgctgcc tggaccgtcg aactggccgc gttattaccc cctaacctgt tattgattta
     1621 aggaatgtaa ggacacgtta tgccaagcgc ccacagttaa gctacgcccg ctggagcgtg
     1681 aagatttacg ctatgtacat caactcgaca ataacgccag tgtgatgcgt tactggtttg
     1741 aggaacccta cgaagccttt gttgaactct ctgatctgta tgataagcgc atattcacga
     1801 tcagagcgaa cggcgctttg tggtggaatg tgacggcgaa aaagccggtc tggtggagct
     1861 ggtggaaatt aaccatgttc atcgccgggc agaatttcag ataattatct ccccggagta
     1921 tcaggggaaa ggtctggcaa cccgtgccgc caaattagca atggactatg gcttcaccgt
     1981 tctcaatctc tataagctgt atctgatcgt cgataaagag aatgaaaaag cgattcacat
     2041 ttaccgcaag cttggcttta cggttgaagg tgaattgatg catgagtttt ttattaatgg
     2101 tcaatatcgt aatgccattc gcatgtgtat attccagcat cagtatctgg cagagcacaa
     2161 aacaccgggt cagactctcc tgaagccgac cgcacaatag cattaataat aatcgatcgt
     2221 atttttgatg gtgtaaaccc gttcgacggc gggttttact ccttcatcaa caatgatcag
     2281 ctgacagtcc actggattgg cgtgactgtc atattcacag ctctgtttta cattaccaac
     2341 tcgttgatta tttagtaaag taaccgctgt gtaatctaat tttttgatcg gatccgttga
     2401 tggcgtggcg ctgacagata atgttttgtc gttacttttc gtggttttac ccagcggata
     2461 cccctgatca tcatagcgat attccatctg catttgtttg ctgctggctt taatcacgaa
     2521 gccattatca tcggtttccc aactcacccc ggcagaaggt aattctgcta gctggcattt
     2581 tccctgtaaa cgtactctct tctccagcgt ctcggcatca cggtaataat tggcgtccag
     2641 taccagagcg accacggtat tattttccag atccagtaat tcgagtgaat caaaacagcc
     2701 ttcttccgac aaagtcccag aaacacgttt cgtcacttca ccttgctcat ccattaatgt
     2761 ctgagtgaaa tcttttaccg gaccacgcag cggatcaaaa tcgaattcat tagagaaact
     2821 ggccatctca ggggtaaatg aaagtgttac ctctgtgcgg tcacatcctg tgaggaatat
     2881 cgcgagtaag catggtagta atttgtattt cacaacagtc accagagagt agagatgatt
     2941 ctcaatcata gtagcaaata cagtacttta cacgttaaat gctatgctta aagaagttat
     3001 cttcgcgtaa ggagcttatg atgaaactct caacctgctg tgccgcgctt ctgctcgccc
     3061 tcgcctcacc cgcggtactc gccgcgccgg gatcttgtga gcgcatacag agcgatatat
     3121 cacagcgcat tatcaataat ggcgtacccg aaagcagctt cacgttaagt attgtaccca
     3181 atgaccaggt tgatcagcct gattcccagg tcgtcgggca ttgcgctaat gatacgcata
     3241 aaattctcta caaccgcacc accagcggta acgtctctgc tcccgcgcag tctacccagg
     3301 atggcgcgcc tgccgaaccg cagtaacaca ttatcgactg aacgccggat atgacacatc
     3361 cggcgatttg aacatacaac ataatctcac cttattactc atacccttct attgatgtgg
     3421 attaataatt cttaacccaa aatgggtaga ctccctctat tgttagcgcg ctaaatattc
     3481 aatgtataaa catttatata acaataaaga acagggagtg agttatgtcc aaaaatgacc
     3541 gaatggtggg catcagccgc agaacactcg ttaaatcaac cgcgacaggt tctctggcgc
     3601 tggctgcagg cggtttttct ttaccgttca ccctgcgcag tgcagcggca gcggtacaac
     3661 aggccagcga aaaagtgatc tggggttcct gttccgtcaa ctgtggtagc cgctgtgcac
     3721 ttcgtctaca tgttaaagat aatgaagtga cctgggtgga aactgacaat accggcagcg
     3781 atgagtacgg caaccatcag ggtacgcgcc tgtttgcgcg gtcgctccat ccgccggcgt
     3841 attaatcatc ccgatcgctt gaatgaccca atgaaacgcg tgggtaaacg cggtgaaggc
     3901 aaattcgaac ggattagctg ggatgaagcc ctggatacta tcgccagtag cctgaaaaaa
     3961 accgtcgaac aatatggcaa tgaagctgta tatattcagt actcttcggg gattgttggc
     4021 ggcaatatga cccgctcttc gccatcagcc tcggcggtca aacgcctgat gaactgctac
     4081 ggcggttcac tcaatcagta tggctcctac agcaccgcgc aaatttcctg tgccatgccc
     4141 tacacctacg gcagtaatga tggcaacagc accacggata ttgaaaacag caagctggtc
     4201 gtgatgtttg gtaacaaccc ggcagaaacc cgcatgagcg gtggtggcat cacttatctt
     4261 cttgaaaaag cgcgcgagaa atcgaacgcc aaaatgattg ttatcgatcc gcgatatacc
     4321 gatacggctg ccggtcgtga agaggaatgg ctccctattc gcccgggcac cgatgccgcg
     4381 ctggttgcgg gtattgcctg ggtattgatt aacgaaaatc tcgttgatca accttttctc
     4441 gataaatact gcgtcggcta tgacgaaaaa accttacccg cagatgcacc caaaaatggt
     4501 cactataaag cctatattct tggtgaaggt gacgataaaa cagcaaaaac gccgcagtgg
     4561 gcttcgcaaa ttaccggtat cccggtagac cgtatcatca aactggcgcg tgaaattggc
     4621 acaacaaaac ccgcttatat ctgctagggc tgggggccgc aacgccaggc aaacggcgag
     4681 ctgactgcac gcgctattgc tatgttacct attttgacgg gcaatgtcgg catcagcggc
     4741 ggaaatagtg gcgcgcgtga atcgacctat accattacga tagaacgcct accggtgctg
     4801 gataatccgg tcaaaacgtc aatctcctgc ttcagctgga cggatgctat cgatcatggc
     4861 ccgcaaatga cggcactccg cgacggtgtc cggggcaaag ataaactgga tgtgcccatt
     4921 aagttcatct ggaactacaa ggacttgaag cggaccctta gtaggcctga ggtcctgcat
     4981 tgagtcaaga ccttgtgtca ttctttgtgc cagacttgtg tcattagacg ggatgtctag
     5041 acggctggtc aggaccctcc ggtggcctcg gtctggactt gaggcgggct atgggggaag
     5101 tctgctggct taatgtatta gatggtcatt cacatttttc tgtcagaaac catcggtccc
     5161 actgtgagta caaacctaag cccccagaag cccacccaca gccttagaca gagtgtcgaa
     5221 tacattcaca ccgagtcgac cggagaggac catatagagg cccacagaga ggaacacgag
     5281 tttatagacc agagtcagga gggaccttag gtcttcggaa gtcatggaca taaagccccc
     5341 cccctatctt ctattgcact accgcactag acaactagta caaaacaaag gcccagacca
     5401 ttggtccaga ccgtagttag ttaatcacag tgacaagcca ccacctgaag tcctgctgat
     5461 attcagtcca gacttacctc tgctcatggt cttacctccc tcggtctcct gaccttgggt
     5521 atcgactaca gcttgctgag cgtcatctgg aggagctaca ggaggagcta caggaggcgg
     5581 gaccgaagcc ccaccttgag tcccaccacc acctgtcagg cccttggcga tggacctgaa
     5641 gcctttacta atgctcttac ccattggtct tacgcttcgg tcttggtgaa cttcaggcgt
     5701 ttgactacag gcaggacggc agtagctgag ccagtgccca tcttgatggt cgtagcagct
     5761 tcagcgacaa cgaacacttt accgaggatt tccaagtcgt gggactgaga gtcgccatca
     5821 cgtgaaagga cgaccgtaat attctcaccg tcgtgacgga agcagccgaa gctcagggcc
     5881 gtgaagcgtt tagccagcat gaatacaccc ttgtcttcac cgttgtaata ggcgatgtct
     5941 gtccccagca tatcgacgat agcgtcttca ccggagaagc cttgcttagc agccagacgg
     6001 gacagtgctt tcagttggct ctcatgagcg ataataccgt agtcagacac ctcagaaccg
     6061 aaagaagact ctgtgttctc atacagagcg tccatgatag cgtctgcctt agcggtcagg
     6121 ctaccagtca ggacaggaag ctctacggtc ttcagcagct tagaggattc cagaacgtcg
     6181 accatgcgag cgttgatggt acggaccaca ttctcacaga tgttcatagc gtcctgctga
     6241 agtgtgccct gagtgaatcc ggtggatgac ttggcgtccc agagcttggt ctgctcagag
     6301 atgagaatag cctgctcgat tggttcgacc acgatttctt gtccagacgg aacggtcacg
     6361 acttcaggtc gcttgccacc acggacagga acgaccacag agcggtcagc cagagtgtga
     6421 gcaatgattg gggtcggtaa gctgttacgg gtcggcagga cttctacagg gaacgggatg
     6481 ttctcttgag cgtctttaag tacaccgttc agcatagcct taaccgtctt agctaactgg
     6541 agggcataga cctgcgggtc agcgttagtc ggcagacctt cattgacctg aatgttgtcg
     6601 gtatcggtga tgcgtactgg ctcaatgcca aactggaaag cgtctttaat cgtagtcata
     6661 ataatcaatc tcctaaatta gtttaaatac tgtgagtcct caaaactgag gagtccctaa
     6721 catcgggtca gggactgtgt gttttacaag ctgtccatcc agttaccgcc tgactcaagg
     6781 tcagcgacaa tagcggcacg ggagcgacca gcggaatggg tagcactgcg gaggctctca
     6841 ccataggtcc ggcgttcagc tttgacctgt tcgcggcgct gtagctcttt ctgccactct
     6901 gcatcggtca tgttctcttc acgcatacgc tcagcctgaa gtcgttgctc tttagcgata
     6961 cgctcagctt cagctcgttt gttcatctgg cggcgaccat aggcggtcat caagtcatta
     7021 ctgaagaagt tgccaatggt cacgccagct tcagccagag cttccagtaa ctccattgcc
     7081 tcgcggctgg tagacggcag gcctgacagg tagcccggaa tgagggtctt gatagcgagc
     7141 gacagcggat agggctgacc gtccagactc acagtgtttt cataaaggtt gatgtcggtg
     7201 actgacagct tccagttgtt gttgttgaac atcataggta attctcctct tgagagttga
     7261 gactatcggt atcgcagacg cagtagcgcc tacgggtctc cccgcatcgg tcatctcaaa
     7321 aggtttaaaa ttaggtttgg tatggtcagg actgcttagc aaaggctcgg cgtaactgat
     7381 tgtctaaatc acggtccacc agcatagcta tcagctcgtc tcgactgtag taagggcgct
     7441 cagggtcttc gactccaagt ctcagcagag acagcagatg acgtgtgggc aggccacggt
     7501 aacgaagggt cgctttgccg ataggtgtac gagggtcaat catagccatg attacttacc
     7561 tccactcaca gcgaccagcg gtcacagctt gatagtctga atactcagcc tgtgagactt
     7621 cacgtccggc gatgtagagg cctgtgccat gacgctggat gaattccttg gtctccggct
     7681 tgatgtcctc agcagacgct acagcagact gcacggtggc gctggaggtc atagacacga
     7741 aggctcgggt tactgccagt ccgtacccaa ggtccaacca ccgaccgaac tctatagcgg
     7801 ctactgggtc agccagccac gaggtcccta agtgacccat gttaatcttt ttaacaaacc
     7861 cgttttctgg gttggttaga cttctaccag ccttggtcct catgaagcga gcaacctcgg
     7921 tactacggtt atcttctacc ttaccaatct gaatgcctac ccggcgtatc tcatccagtc
     7981 gaaacaatcc gtcctgactg gtgatgtcct gaccgaagat atgaatggtg tattcctgct
     8041 tattgtcaga agtcataaga ggcacctccc gccagagctt cggcaaagga cttgaagcgc
     8101 tgttcttggt cagctttcgc ctgaaggcgt tcggcatcag gtgggaccag cttatcaaag
     8161 gtgccgtttt cgtaatcttc agttaggacc tgaaggcggc gggaccattt gctctccacc
     8221 tgcatcatcg cgttagagat gtgcttcaag tcggcatcag agcgtgacgc tatccaatcc
     8281 atctcttctg agtcaccctc acaggtgtaa ggattgttga aatagaagat gtcctgcagg
     8341 ccacgcttca gctcttcctc ggtgtgtgtc tggaggtatg actggatagg ctccgcgtac
     8401 atctcccgga ttttctcata gtcccagtct tccgcctggg cataccatcg ggataacttc
     8461 agcaggtcta agttatagcg acagcaatcg acactggact ctgcattggt cagcaggatg
     8521 attaagaact ggcggttagt tactggtaat tcggtattca tacaaagatg ctcctttggt
     8581 aaagcacttt ctgtctccac atggagtccg caccattcag acgcatccta aacggaccca
     8641 tcagaaagtg cttaacgaaa gtgaatggtt aaatgttgac ggtctggagt gaccatgttc
     8701 tccatgtacg tataaaggtg cgaccgcatg tcaggacgag ctgtaaggac tccagatggt
     8761 gagcggtgat agacttaagc caatgtagtg agtagacgag ctactaaccg agacgactaa
     8821 agaacgctaa cgagctggag gtcgatacag gaggtctgtc agaggcggtc acgggccttg
     8881 attacttagg agagatactt aatgtcggtt gaccagatgc tcgcatgttc tgacgcatca
     8941 gactcagagg cggaggctgg tctccctata gtgatagttt agggtccgta atcatgaacc
     9001 agctttactg gcgtggtact ggacggattt actggcgctg acgacggtag catagacgta
     9061 gcgagcgtag cgagttgaaa ccataggttc acccaaggca acacgcttac ggctcaggga
     9121 ctcctgcaca gcgcttacag ttaatgtgtc gtgcgttagt agttgggtca gaatcaagta
     9181 tgggctgaca ggcttggtat tgcttgccat tgattcgttc aggcgaccag caccagtaag
     9241 gacatcatcc agccaaccac tcaggtcgcc ctgaccatcg aatgacagca ggtccccgca
     9301 gtgctgctgc aagtaggctt tggtgaacgt gttgtcgtga gttgcgattg gtcgtgccat
     9361 agagtatctc ctgtagttga attagttggt tcccctatag tgataatcaa gtccatcact
     9421 ggtgatgcat acagtagcgc gacaggatgt gactcacagg ttgaaccgaa tgtcaagagg
     9481 agaccatagg aatttggagg gactggagtt taggacgcag tttaaataag accacagaca
     9541 gacttagagt cggacagccg aaggcggacc agtgggccta tagtcaggag ggattatgat
     9601 tattatcacc ctccctaact tcagtcagac ttcagtgacc caaggtctca accatcaagc
     9661 cgtatatggt ctccaccttg ggtctcccta cttaaagtaa agctgtctta agcacatcag
     9721 ccagcctacc cacagcgtgt cctttcccgt acaggtcata cgtaattgac tgactgctgt
     9781 gacctaagat gccttgagcg tccaccagat tgactccacg ctctttcatt cgggtcgcca
     9841 gccaatgtct cagactgtgg agagagacat cctgagagtc tcctagggcc tcagtcagga
     9901 cccttgagtt aaaccagcta gagtaggcgc tcggagtcat cccgaagaga ggaccctcat
     9961 caggctgcat gtctacccac gacaggaagg acgtcaggtc aaacccataa gcgccatcgg
    10021 ttaacggcac aagtcggact gaatgtttgt tcttcacaga cttcccgtcc ccatcttcat
    10081 tgatgtcgat acagaccagt ccattgtcca aggttacaat gtcacgttta gtcaggtggc
    10141 agacctcagc agaccgagcg ccagtgacca cagacaccag agacgcccat ctacgggcct
    10201 cagaggtcgt gtgagccgtg tgtcgtgtga actgata
//
LOCUS       DAJTSI010000138         9964 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000138 DAJTSI010000000
VERSION     DAJTSI010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9964
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.138"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..448
                     /locus_tag="OO327_003227"
     CDS             <1..448
                     /locus_tag="OO327_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HCR6019661.1"
                     /translation="VDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTL
                     PIGSEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFV
                     IGFPLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            733..1356
                     /gene="rcsA"
                     /locus_tag="OO327_003228"
     CDS             733..1356
                     /gene="rcsA"
                     /locus_tag="OO327_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR6019662.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(1400..1588)
                     /gene="dsrB"
                     /locus_tag="OO327_003229"
     CDS             complement(1400..1588)
                     /gene="dsrB"
                     /locus_tag="OO327_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR6019663.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            1751..1978
                     /gene="yodD"
                     /locus_tag="OO327_003230"
     CDS             1751..1978
                     /gene="yodD"
                     /locus_tag="OO327_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR6019664.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            2276..3091
                     /locus_tag="OO327_003231"
                     /pseudo
     CDS             2276..3091
                     /locus_tag="OO327_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(3088..4782)
                     /gene="yedQ"
                     /locus_tag="OO327_003232"
                     /pseudo
     CDS             complement(3088..4782)
                     /gene="yedQ"
                     /locus_tag="OO327_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(4953..5135)
                     /locus_tag="OO327_003233"
     CDS             complement(4953..5135)
                     /locus_tag="OO327_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR6019665.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(5214..6131)
                     /locus_tag="OO327_003234"
     CDS             complement(5214..6131)
                     /locus_tag="OO327_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR6019666.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            6304..7224
                     /gene="yedA"
                     /locus_tag="OO327_003235"
     CDS             6304..7224
                     /gene="yedA"
                     /locus_tag="OO327_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCR6019667.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(7213..7683)
                     /gene="vsr"
                     /locus_tag="OO327_003236"
     CDS             complement(7213..7683)
                     /gene="vsr"
                     /locus_tag="OO327_003236"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR6019668.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(7664..9082)
                     /gene="dcm"
                     /locus_tag="OO327_003237"
     CDS             complement(7664..9082)
                     /gene="dcm"
                     /locus_tag="OO327_003237"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR6019669.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(9149..9689)
                     /locus_tag="OO327_003238"
                     /pseudo
     CDS             complement(9149..9689)
                     /locus_tag="OO327_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(9729..>9964)
                     /locus_tag="OO327_003239"
     CDS             complement(9729..>9964)
                     /locus_tag="OO327_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019670.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT         2447 a         2630 c         2380 g         2507 t
ORIGIN      
        1 tgtcgatcct ggcagccatc tcaatatgcc cgttttagcg cgcatcatgg atatgctggc
       61 attactgctg ttcctgacat ttaacggtca tttatggttg atttcactgc tggtcgatac
      121 ctttcacacc ctgccgattg gtagcgaacc gttgaacagc aatgcgtttc tggcacccac
      181 caaagcaggg agtttgattt tccttaacgg gctgatgctg gcgttaccgc tcattactct
      241 gttgctgacc ctgaatctgg cattaggttt actgaatcgt atggccccgc aattatccat
      301 ttttgttatt ggatttccat taactctgac tgtcggcatc tctttaatgg cggcattaat
      361 gccgttaatt gcgccttttt gcgaacattt attcagtgaa atttttaatt tgctggctga
      421 tattattagt gaattaccat taatataatt ccgtaacgtt tatcatgcta ccctaaggat
      481 tatccgaaaa ataatatctg cgaacatctt ccaggatact cctgtagcga aatatttgtt
      541 ttaagctcac tcacatatcg caacatttac tttaagacaa ttccaggcaa attatacaac
      601 actttacggg atagtaagtc cgcctgaaaa attgcgagag tggcgcatta ggtgacccat
      661 gttgttccgt ttagtcatga tgaaatattc aggtaagggg aattatcgtt acgcattgag
      721 tgagggtatg ccatgtcaac gattattatg gatttatgta gttacacccg actaggttta
      781 accgggtatc tgttgagtag aggggttaaa aaaagagaaa tcaacgacat tgaaaccgtt
      841 gatgaccttg ccatagcttg tgattcacag cgtccttcag tggtgtttat taatgaggac
      901 tgtttcatcc acgatgcttc taacagtcag cgcatcaagc acatcattaa tcaacatccc
      961 aatacgttat ttatcgtttt tatggcaatt gccaatgttc attttgatga atatctattg
     1021 gtcagaaaaa atttattgat cagttctaaa tcgattaaac cggaatctct cgacgatatc
     1081 cttggcgata ttctgaaaaa agagacaacg ataacctcgt ttttaaatat gccgacgtta
     1141 tcattgagcc gaaccgaatc gagtatgttg cgaatgtgga tggcaggtca gggaaccatt
     1201 caaatctccg accaaatgaa tatcaaagcc aagaccgttt catcgcataa aggtaatatt
     1261 aaacgtaaga tcaaaacgca taataaacag gttatctacc atgtcgtccg actgacggat
     1321 aatgtgacta atggtatttt tgtcaacatg cgctaacaca ttctgactgg tggtttccca
     1381 ccagtcaggc tgaataagat tactctgctt tctcaacaaa gataccgtcc tgatgccctg
     1441 cttcattaaa gaaccagatg ccgagcgggt agtcttccag cgcaaccagg tacattgtgc
     1501 cttcactaaa ctcctcaact gccagtacca ctccaggacg acgcggaccg ccatccgttt
     1561 tgactgttac ccgatcattc accttcatcg ttttcctcct gtggctttgt gccagtgtag
     1621 aacaatttcg tttttctggc agcgccaagc gcgcgcgagt gctgattttc tcgacggtct
     1681 atacttaaga gatgccaggc ggacttaacg actggcggca acaacagagt aacggttgcg
     1741 aggaaagatg atgaaaaccg caaaagtgta cagcgatacc gcaaaacgtg aggtcagcgt
     1801 cgatgtcgat gccctgctgg cggcgatcaa tgaaattagc gaaagcgaag ttcatcgcag
     1861 ccagaacgat tctgaacacg ttagcgtcga tggacgtgaa tatcatacat ggcgtgaatt
     1921 ggcggatgcc ttcgaactgg atattcatga cttcagcgtc tctgaagtga atcgttgaat
     1981 gcacaataaa aaaatcccga ccctgagggt gtcgggatga aacttgctta agcaagaagc
     2041 acttaaaaaa ttcgttacac caggaaatct gatgtgttca tcaccttatc tgcaattttt
     2101 ttcgctgaca agaaaatatt cgcgctatga atgattaacg ctacttatga atagaaatgt
     2161 gacgcgcgtc accccttggt ggctctggtg tcaacgggat cgctcccgaa aaaatcatct
     2221 ttgattattt gtgctgtttt gagaatcatc ctggtaaggg gagtactgcg cgaccatgtt
     2281 ttcaattcaa caaccactac tggtttttag cgatcttgat ggcaccctgc tggacagtca
     2341 tagttatgac tggcaaccgg cagccccctg gctcagccgt ttacacgaag ccaatattcc
     2401 cgtcattctc tgtagcagta aaactttagc ggaaatgctg tacttgcaaa aaatgttggg
     2461 gctacaaggc ttaccgctga ttgcagagaa tggcgcagtg atccagcttg ctgaacaatg
     2521 gcaggatata gacggttttc cacgcatcat ctcaggtatt agccatggcg aaatctgcca
     2581 ggttttaaat acgctacgcg agaaagaaca ttttaaattc acgacttttg atgatgtcga
     2641 cgatgcaacc attgccgaat ggacgggatt aagccgtagc taggcggcgc tgacgcagct
     2701 gcatgaggcg tcggtaacgc taatctggcg cgacagtgac gagcgtatgg cacaatttat
     2761 cgctcgtctg aacgaactgg gcttgcagtt tatgcagggt gcgcgcttct ggcacgtact
     2821 ggatgcctct gccggaaaag atcaggctgc caactggatt atcgcgacct atcaacaatt
     2881 gtcaggcaga cgcccaacca cacttggcct gggcgatggg ccaaacgatg cgcctttact
     2941 ggaggtaatg gattacgcgg tgattgtgaa agggctaaac cgtgaagggg tacatctgca
     3001 tgatgaggat ccggcccgcg tctggcgaac gcagcgtgaa ggaccggaag gctggcgtga
     3061 agggctggac cattttttct ccgcccgtta agcgttatcg ctcgcgaata cccgattgcg
     3121 cccggcctgt tttgcgaggt aaagccgacg gtcggccaat gactggagtt gttcaaaatc
     3181 ataatcaccg gtttcctcgc tgctacttac ccccagcgag gcactgatgc gtatcgttgt
     3241 actcttggcg atcagcatct ctttttcatt taacttcagg cgaatacgtt ctgcgacctc
     3301 cgcagcctgc gtcagattcg cgcctggcag aatcacacaa aactcctcac caccgacccg
     3361 cccggcaacg tcctgcgcac gcaaggaact gctaattaat ccggcagcat gagaaagaac
     3421 acggtcgccc gcctgatgac caaagcggtc attaatcgct ttaaaatggt caagatcgac
     3481 ctgaatgaca gaaaaaggat gttggtgcgt ctgacacaat ttagcgagcg gacgggcttt
     3541 ttcgaacagt gcgccacggt tatataaacg cgttaaggtg tcgtgccacg cctgccactg
     3601 caacgagctt tgcagaacat acatgttgct gaccatccgg cgaatcacat accaggagag
     3661 gagtaacatg gtggtaaaga gcgcccacag cagggttaat gcaatgctga tactgccgaa
     3721 atcgccgcgc acgccttcgc ttagcgtatg gacgcgcgcc agcacaccgt cgaaatggtc
     3781 cagacgttcc cagctaacat agcgactgtc catacgaatg ccgccccgcg tgtcatgttc
     3841 cattgcctgc gccagcaagg ccagttcacg aggatcaaaa atattccctg ttggatggtc
     3901 aggattggaa gaggtcaaaa atctcagctt actgtcatag agctgatact caccatcgag
     3961 gtttttatcg atggcgtttc ttaaaaattg ctgcatagta cgcacgggaa tactcatccc
     4021 cagcacgcca taccagtagt tattactgtc taccggaaca ctgacggtaa cctgcggttc
     4081 agtattgctg gcgtgttccg gttgcgaagt gaaccagcgt accgcgcggt gacgattttc
     4141 tcgttgcgaa tggccgataa accagggttg ggtgacatag ccgtaataac gcgttggtac
     4201 attgcgcgta aacaaggtcg gctgcgtcga aacgtaaaat ccagcacgcg agacatacat
     4261 cgcctgttca accatcgacg aggagttgtg cgccagtcgc agcaagtaac caacttccag
     4321 tgcagcggta atttcattgt cgaggctttc attttcgcga gacaggaaat tcccctcgct
     4381 gactaatgca tccgatacgc cattgactgg cagggtgcgc cgtcggttga gttcgatttg
     4441 ccaggcgtgc tcgtcgcgat gctgttcgaa ctcggttacg gcattacgca gagaggtgaa
     4501 atccagtggc gccacgagag cttcgcgcat gccattacgc aaaaagacca gtttgtcgac
     4561 gttatactgc aaatgcttat cgagcgcgtt ggctacgttt tccagatgat tacgctagct
     4621 ggagatatag gcatcttcca gcaccaccac ttcacgccag gtgagcaagg tggaaaaaag
     4681 caataccacg ataaagcaga gattaacgac atgaccgggc cccaggcggc gtgcgagttt
     4741 tttcaaccag ctctggtttt ccatttttgt ctcgtgctgc accctgacga ctcccaacct
     4801 gcttttttat gattctggca taacccggca atagcgtacc gcaaaacagc gataatttta
     4861 taatggtggc atgataagac gcgactatca ttcattgcag gcattgggac accaggccgg
     4921 atgtgatccg gcctggtgtt aatgataccc tttcaaactt cttccgcaga acgactcccc
     4981 tcccccggaa caagatcggt ttcatgaaaa gcttcccgct tgaccccgta accgtcatac
     5041 caacgacact caaccatacc gctggagtat ccagtgacaa tcatccgcgg gccgccctct
     5101 ttaaccgtaa cttcctcact aaccataaag ctcatactcg cctccttttt tcgagtgaaa
     5161 cttgttcacc ttagttgaag atggcgaatt ttgcataata gccagtgcga gtattaatgt
     5221 gcctgaccgc gcattttcgc tactgctttc actcccagca ccacgatgcc gccgatgata
     5281 aatccaagaa tcagatttaa aacagtcggt aatatcatcg ccaccactgc actttgctgc
     5341 ccggcgatat gttcaatagc gtgatgcagc ggcgcaatac catgtaccac aatcccgccg
     5401 ccgacgagga acatcgccag cgtgccgaca atcgataatg ctttcatcag ccaaggcgcg
     5461 ataatcaata accctttacc taatgcctgc atcagcgcgc tagatttttc cgccagccaa
     5521 taccctaggt catcaatctt aacgataacc cctaccagac cgtagacgcc cacggtcact
     5581 accagcgcga tgcctgaaag caccagcacc tgattaagca acggcgcctc tgccacaatc
     5641 cccagcgtga tggcgacgat ttccgcagac aaaataaaat cggtacgaat cgccccttta
     5701 attttgtcct tttcaaactt cagcggatcc tgcgccgcca gcttctccag acgctgctgg
     5761 ctctgcgctg gatcttcttt atgtttacgc gcctccagca tatgcagcac tttctctacc
     5821 ccttcaaagc agagaaacgc gccaccaatc atcaacagtg gcgtaatcgc ccacgggata
     5881 aacgcactga tgatcagcgc cagcggcacc agaatcactt tattgatcag cgatcctttc
     5941 gccacgcccc agaccacggg aagttcccgg ttggcccgca cgcctgaaac ttgttgcgca
     6001 ttgagcgata aatcatcccc taatacaccg gcggttttct tcgccgccag tttgcccatc
     6061 acagagatat cgtccagcag tgtggcgata tcatcgagca acgtcagtaa actactcccg
     6121 gccaaaagca ttccctctca tttttattgt tgaagggtgt aagtatgaag caaaaacggc
     6181 agctctgata cttgcttcac aggtcaacat ttatttaaca atcaagtgca attatatctc
     6241 tcgccagcag aatgattttc acgtttacta tgaaccacct ttttatttcc gccgtgaggg
     6301 attatgcgtt tccgccagtt gttaccgctt tttggcgcgc tgtttgcgtt gtatatcatt
     6361 tggggctcaa cctattttgt cattcggatt ggcgtgggaa gctggcctcc gttaatgatg
     6421 gcgggcgttc gattccaggc agccggtatt ttattgctgg catttttgct actgcgcgga
     6481 cacaaacttc ccccgctacg tccgctgctc aatgccgcgc tgattggcct gttattgctg
     6541 gctgtcggta atggcatggt gacggttgcc gaacatcaaa atgttccttc cggcatcgcc
     6601 gccgtagtga ttgcaaccgt gcccctcttt accctgtgct tcagtcgcct gtttggcatt
     6661 aaaacgcgca aactggaatg ggtgggtatt gccattgggc ttgccggaat catcatgctc
     6721 aatagcggtg gaaatttaag cggcaatccg tggggcgcga ttctgatttt aatcggctcg
     6781 attagctggg cgtttggctc agtttatggc tcgcgcatta ccttacctgt agggatgatg
     6841 gcgggtgcga ttgagatgct ggcggcaggc gtggtgttaa tgatcgcatc gatgattgcg
     6901 ggtgaaaaac tgacggcgct cccttccctg tcaggcttcc ttgcggtcgg ctatctggcg
     6961 ctgtttggtt cgattatcgc cattaacgct tatatgtatt taatccgtaa tgtcagtccg
     7021 gctctcgcca ccagctacgc ctacgttaac ccggtggtcg cggtcttgct gggtacggga
     7081 ctgggtggcg aaacgctgtc gaagattgaa tggctggcgc tcggcgtaat tgttttcgcg
     7141 gtggtactgg tcacgttggg aaaatatctc ttcccggcca aacccgttgt cgcgccagtt
     7201 attcaggatg catcaagcga gtaaatgaat cccctgcgtg tcgatctgcg cgctggcacc
     7261 ttcgccgcag atccactctt ccagacgctc ggtaagcgcc gcatccgtca gcttctcgcg
     7321 cctacgtaac gcacactccc agacaatcag tacgcgccag ccgagctcct gcaagcgact
     7381 gatatcgcgg cgatcgcgtt caacattttt acctatcttc tccagccaga actcagttcg
     7441 agtcgcaggc actttaaaca gatagcagtg atgatgatgc cagaagcagc cgtgggtaaa
     7501 tatcacgcag cgatattcat caacgacaaa atccgggcgt ccgggcagac tggcgtcctg
     7561 aacgcgaaac gcaaggccct gcccggttaa caaactggcg aggcgcttct caatcgccgt
     7621 atcacgcgtg gcaatcgcgc gcatattttt gctgcgggtg gccttatcgt gaacgtcgac
     7681 catgttgtgc ctcttgctga cgcaacgcca ccgcctgttt gatttttggc tcaagcagtt
     7741 ttgccaccgc ggcaaagacc ggcacgacca ccgagttgcc gaactggcga taggcctgag
     7801 tgtccgaaac cggaatgcgg aatttcgctt ctcccggcgc ttcaaaaccc attaagcgcg
     7861 cgcattcacg aggcgttaac cgacgtggac gatgttgctg attcagcgga tcgtcaaagt
     7921 ctttctcacc cgtggccata tcccaaccgc gatcgattaa aatttccgcg ccatctttgt
     7981 aataacgagc agacagcgta cgggtgacgc tttgcggatt gttcggataa accattccat
     8041 aaccgaagcc gttaccgcgc gcctgatgtt ttttcgcata tcgatagagg tacttccaca
     8101 gcaccggcgt caggatatat ttcgcctcaa ccatcgggtc cagcagctgc gccagcgtca
     8161 ctcgctgtgc agggaaacat tcgctgatat cacgcagagt aaaatcggct ttcagattca
     8221 gatcgcgacg aaaacccacc agcacgatgc gttcacggtg ctgcggcagg aaatgtttac
     8281 catcgatgat tttcggatcg tccggcccgt tatcttctgc atcggcaacg tcatagccca
     8341 gttcgtccag cgtctgcatg atgatgcgga acgtttttcc cttatcgtga ctcttcaggt
     8401 ttttgacgtt ttcgagcaca aacatcgccg gacgacgcgc gtcgataatg cgcaccacat
     8461 caaagaacag cgtgccctgg gtatcgcagg caaaaccgtg cgcccgcccg agcgagttct
     8521 ttttcgatac gccagccagc gaaaatggct gacaagggaa accggccagt aaaacatcgt
     8581 gttcaggaat gtgttgacga atatgttccg ccgccgcctc atcactcacg ccttcttgat
     8641 gactgagggt gatgtcgcgg atatcttcat taaaatgatg cgtcgccggg tcgcaataat
     8701 ggttggcttt ataagtgcgt accgcatgtt tgttccattc gctggtaaac acgcactgtc
     8761 caccaatcga ttcaaaacca cgacggatgc cgccaattcc ggcaaataga tcgataaagc
     8821 gaaacgcata atgcggatga tgttccggtg gtttgggtaa caacgtttgc agatgggcaa
     8881 actctttctc acttaaacgg tgccatgccg agtcattcgc cagcgcacgt tttaaaattg
     8941 ccgcgctcca gtgattctca cccacaccat taagctgcgc caccagcatt ttaacatcat
     9001 aaatttgcag cagtttctcc agcattgcct gtgcggcatt cccggtgctg tatgaatcgg
     9061 ttactgatat attttcctgc atagatttaa ccggccatct gagatggccg acaggttaac
     9121 ataattactg ccgtttaggg ccacgcgctc agccagcggg tgaaaacgca tctatcacct
     9181 tgtgatcgat accttcattc tcccccgcca gttcggcacc gagcttcgcc atcaaacagc
     9241 gccaagctca atgcccctga aacggcaaat acgcgcgcca ggccaatcgc ccccagggct
     9301 tccagccgat ccgcatcctg gacgatttta gcttccgttg ttaagggggc gatttgcgcg
     9361 ctgaaactgt gagcggcaat ggcatgacaa acggcctcaa ttttctccgc cggaaattgc
     9421 acgaactctt cacggagcag gcgacgcgtc tcttctgccg ccaggattga agaacgctgc
     9481 cgttgcggat gatttttcgc caggctcacg atatcgtgaa aataacaggc ggttaaaatc
     9541 accagcatat cgacatcgtc atctgccgcc agtttctgtg ccgtagccca gacacggcga
     9601 aaatgacaca ggtcatgcgc cgcgtcctga tgttgatgat ggtttttcaa ccagttctcg
     9661 aactatgcct gccagtgttg taactccata cgcgctccgt tgccaaagag tctcaacctt
     9721 agcagttttt attcatagcg tctgcaacgt gcgggacgag gtcgggtgta ccatgcaatg
     9781 ccgcctaaca aggcaccaac cagggccata aacagaaatc cgaccaatgc tcctaaccac
     9841 ttccccgcgg tagatcctaa atcactttca aatcccgaca cgggggcatc ggggatccgg
     9901 ctcatcaccc agatgtatcg cgccattgcc cagacgaaat agccacacca tgtatagaaa
     9961 gccc
//
LOCUS       DAJTSI010000139         9935 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000139 DAJTSI010000000
VERSION     DAJTSI010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9935
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.139"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1023)
                     /gene="bglB"
                     /locus_tag="OO327_003240"
     CDS             complement(<1..1023)
                     /gene="bglB"
                     /locus_tag="OO327_003240"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCR6019671.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVG"
     gene            complement(1042..2919)
                     /gene="bglF"
                     /locus_tag="OO327_003241"
     CDS             complement(1042..2919)
                     /gene="bglF"
                     /locus_tag="OO327_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCR6019672.1"
                     /translation="MTELAGKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGQLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(3053..3874)
                     /gene="bglG"
                     /locus_tag="OO327_003242"
     CDS             complement(3053..3874)
                     /gene="bglG"
                     /locus_tag="OO327_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR6019673.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(4161..4886)
                     /gene="phoU"
                     /locus_tag="OO327_003243"
     CDS             complement(4161..4886)
                     /gene="phoU"
                     /locus_tag="OO327_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR6019674.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4901..5674)
                     /gene="pstB"
                     /locus_tag="OO327_003244"
     CDS             complement(4901..5674)
                     /gene="pstB"
                     /locus_tag="OO327_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR6019675.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5766..6656)
                     /gene="pstA"
                     /locus_tag="OO327_003245"
     CDS             complement(5766..6656)
                     /gene="pstA"
                     /locus_tag="OO327_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR6019676.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(6656..7615)
                     /gene="pstC"
                     /locus_tag="OO327_003246"
     CDS             complement(6656..7615)
                     /gene="pstC"
                     /locus_tag="OO327_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR6019677.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7702..8742)
                     /gene="pstS"
                     /locus_tag="OO327_003247"
     CDS             complement(7702..8742)
                     /gene="pstS"
                     /locus_tag="OO327_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR6019678.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8990..>9935)
                     /locus_tag="OO327_003248"
     CDS             complement(8990..>9935)
                     /locus_tag="OO327_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6019679.1"
                     /translation="GYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYY
                     TSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYN
                     CNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADV
                     YIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGI
                     LNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVY
                     GEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         2562 a         2586 c         2294 g         2493 t
ORIGIN      
        1 tccaaccgga tcaatttgcc acccccactc tgaacttttc agatgcggat tggggatcat
       61 attcagtatg ttgccctgcg catttttatt aatgctttcg tcgtgggaaa cacaaccagt
      121 catgtaataa ctaaaagaga tgaaatctac agtatgtttt aaatcttctg cgtcactttc
      181 agtcatctca atggtgatat tgtggtcgcg gaagaaacgc tgcatatagc cgggatattg
      241 gccacgcgcc tgaacatcac caaagaacat ccagcgccgg ttctcttcca tggcctgcaa
      301 catatcctgt ggctggcagg tgagggggta aaccagccca ccgagaagca tattgccgat
      361 tttcgcttcg gggatcaggc tatgataggc tttaactgcc cgcgcactgg caaccagttg
      421 atgatggatc gcctgataaa cttccgcctc gccactctct tctgccagcc ccacgcccgt
      481 gaatggcgcg tgtaacgaca tattgatttc attaaacgtc agccataacg ctactttatg
      541 ttggtagcgg gtaaagaccg tgcgggcgta atgctcgaag tgatcgatga ccgcccgatt
      601 agcccaaccg ccgtagtttt tcaccagccc atatagcatt tcgtaatggg ataacgttac
      661 cagcggcttg atccccgcct gcgccatttc atcaaacagc cgatcgtaaa acgctaaccc
      721 cgcttcattc ggttcggctt cgtcgccctg agggaaaatt cgcgcccagg caatggaaat
      781 acgcagacag gtgaagccca tctcggcaaa taacgcgata tcttccgggt aacggtaata
      841 aaaatcgatg gcgacatctt tgatattctc tttccccagg atgcgcggtt ccatttttcc
      901 cattacgcca tgaggctgta aatctgaggt cgtgatccct ttgccatctt ccagccaggc
      961 accttccacc tgattggcag ctgttgcgcc accccaaaga aatgtttctg gaaatgcttt
     1021 cataattaac tccttttatc gttagcgaat gatggataac agcggttcac ctgcgcttat
     1081 ctgcgccgtg ccgtggggta atacgtctgt aaaatcatcg ctattactga ttaataccgg
     1141 cgtcgtcaga tcaaatccgg cctcgcgaat agcagggata tcaaaagaaa tcagccgatc
     1201 gcctgtattg accttgtcac ccacgttgac gtgagcggaa aagaatttgc cgtccagttt
     1261 tacggtgtcg ataccgacat gaatcaggat ctccacacca tcatctgact caatgccaat
     1321 ggcgtgtaat gtggcgaaca acgaagcaat tcgacccgca accggagaac gcacttcacc
     1381 aaccgagggc agaatggcga tacctttacc caacaggcca ctggcaaacg tggtatcagc
     1441 gacgtgaatg agcggcacaa tctctcccgt catcggtgaa cagataccgc cctgctcagg
     1501 tggtgtaata acctctggtg ttttctcttg cggggcaccc tgcgctggct gacgtttact
     1561 ggtgatgaaa tgaagcatca acgtaccaac aaatgcgcaa cctatggcaa tgacaccgcc
     1621 aataacgctg gcccagacgg tgaaatcaat ccccgttgac gggatggtct gcatgaaggt
     1681 gaaaatactt ggcaaaccaa aggagtaaac tttcgtttgc gcgtagccaa tgatggtggc
     1741 ccccaatgct ccactgatac aggcgataac aaaggggtac ttacgcggca ggttgacgcc
     1801 atataccgct ggttcggtga taccaaacag acccgtcaac gccgctgatc ccgccaccac
     1861 ttttttctgc gcatcgcgtt cgcagaggaa gacgccgagc gccgccccga cctgcgccat
     1921 aatggcgggc attaacagcg ggatcatggt gtcgtagccc agcacggtga agttattgat
     1981 acacagcggc accaggcccc tgtgcagtcc gaacatgacg aagatttgcc agaagccgcc
     2041 cattaccgcg cccgcaaatg caggaaccgc ctgataaagc cagagataac cggcggcaat
     2101 catttcgctt atccaggttg atagctgccc caccagcaga aaggtgacgg gtgtgataac
     2161 catcagacat agcaatggtg tgaagaaatt tttgattgcc gacggtaacc acgcattaag
     2221 tcggcgttcc agaatgctgc acaaccaggc agaaaaaata atgggaataa ccgatgacga
     2281 gtaattcaac aatgtgaccg gaatacccag gaaatccagc cccggcgcat ccgctttttg
     2341 cccgttctcg aaggcagtca gaattaatgg atgcactaac gctccaccaa tcaccatggc
     2401 agtaaatgga ttaccgccga agcgtttgcc cgcggtgtat cccaggatta tcgggaagaa
     2461 cccaaacaag gcatcactgg cgctgaataa aattaaataa gtaccacttt gttcggtcgt
     2521 ccactgaaaa gtgagcgcca gagccagcat acctttcagg atcccggttg ccgccatcag
     2581 accgatcaga ggcgtaaaaa tacctgaaat aacataaaca aagcggttta gcagattacc
     2641 tttctcatca ttttccggtg cctgttgcgc tttttcgcca aggcctgcca cgctgttaac
     2701 cgccaggaag acatcggcca catggttacc tatgaccacc tgaaactgac caccgctttc
     2761 caccaccata ataataccgg gggtcttttt cagtacctca gcttgcgctt tgctttcatc
     2821 ctttaactta aaacgtaatc gcgttgcgca atgcatcaga ctcacaatgt tatctgcgcc
     2881 cccgactcct gcgactattt ttccggctaa ctccgtcata acttgccctc taccgctttg
     2941 cggcaaaact ccaaaaaaaa acctgaaaaa aacggcctga cgtgaatcaa gcaatttttt
     3001 tcaggttttg cccgcttagt gcggtaacaa tcctttactc agtaataata tttcagtgtt
     3061 ctttgcgcac gcgctctata tttatggcta aaaacataat ctctgcgggt gaaattttac
     3121 gttgatactg caaaccaata aaaatggcga tccgttccgc acattgccat gcttgcgggt
     3181 aattttgttt tactgcttgt tgtaatgatt catcactatc gttaattgaa gcatgttcaa
     3241 gaatacgcca ggataaaaac ttcagatgtg taaccagtcg ctgataactc aagctttctt
     3301 cctggtaatt aaggctgaac tgaaatttta ttaattgcag catttcgcgc attaactgcg
     3361 tgacacctgc aacatcctcc atatttccgc tcatttgggc actgaccaga tgcatggcaa
     3421 taaagcccac ttcatctttc ggtaactgca cgcccaaccg tttatcaata atggttaacg
     3481 cttcttcccc tagctggaac tctttcgggt aaagccgctg gatatcccac agcaacacgt
     3541 tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt tagcgagata taaatactgt
     3601 cctgtaattt tcccaagcgc tcctgcgcta aagagataag acgatcacag gttgccatca
     3661 cctcaagagg aatatgactt aagagttcgc ttaatcgccc gttcagttca tgactgctca
     3721 aggcatactc tttttctatt ccacttgagt taattctttc gccagcgcgt ttttgaaagc
     3781 caattccgcg ccccatgacg actttttccc gctgttgatc atcaataacc accacaacat
     3841 tattgttgag aattttggtg atttgcatgt tcatagcaag gacctttttt ataaacaaaa
     3901 aaaaaccgac ttcaccagta ttctctggtt atgtcaggtt ttgcctgcga atgcagtaac
     3961 aatccagtca tttattaatg gtttttataa cgaacatcca ggttcggaaa ttaatttaat
     4021 tgcgtgcaga gaaaatagca atgcgctatt gataaaaata tgaccatgct cgcagttatt
     4081 aactttgtgt aattttagga atttataaag ttatatataa caaatcccaa taattaagtt
     4141 attgggattt gtctggtgaa ttatttgtcg ctatcttttc ccgccagcag tttatccagc
     4201 tcatcaccgc ctacatgacg gaaatcctga cccttcacgt agtagaagat aaactcacaa
     4261 atattctggc agcggtcgcc gatacgttcg attgaacgcg cacagaacag cgcggtaagt
     4321 acgctcggaa tagtgcgcga atcttccatc atgtaggtca tcagttgacg aacaatacct
     4381 tcgtattcct gatcgacttt tttatcttca cgataaatac gtaccgcttc gtcaatgtcc
     4441 atccgcgcga acgcgtccag cacgtcgtgc agcatctgga tggtatgacg gcccagcgac
     4501 tccagactta ccagcaacgg ctgatgctgc tgggagaatt tctccagcgc agtacggcag
     4561 attttgtccg ccacgtcgcc aatacgctcc agctcggcaa tggttttact gatcaccata
     4621 accagtcgca ggtcgctcgc cgtcggctga cgtttggcga taatgcgcac gcaggcttca
     4681 tcgatcgcca cttccatcat gttgacgttc ttgtcgcctt cgatcacgcg cttcgccaga
     4741 tcgctgtcct ggttatgcat cgcggtgatt gcatcagaaa gctgctgctc caccatgccg
     4801 cccatggtca tcacctgcgt gcggatactt tccagttcgg cgttgaactg gccggaaata
     4861 tgtttattaa gattgagact gtccataacg cactcctgaa tcaaccgtaa cgaccggtga
     4921 tgtagtcttc tgtttgtttc ttcgctggct tggtgaacag atcgtccgtg ttgctgaact
     4981 caatcaattc gcccaggtac ataaatgccg tgtggtcgga acaacgcgca gcctgctgca
     5041 tgttgtgggt gacgatcacc acggtgtaat cctgcttcag ttcggtgatc agctcttcaa
     5101 tacgtccggt agagatgggg tcgagcgccg aacacggttc gtcgagcagc agcacttccg
     5161 ggcgaatggc gataccacgc gcaatacaca gacgctgttg ctgaccacca gagagagagt
     5221 aaccgctctg gtgcaattta tctttggttt cgttccacaa tgcggctttg gtcaatgccc
     5281 actgcacgcg ctcgtccatg tcggcacggg agagcttctc aaacagacga acgccaaagg
     5341 cgatgttgtc gtagatggac atcgggaacg gcgtcggttt ctggaacacc atgcccactt
     5401 tcgcacgcag cagggcgata tcctgagagt tggtcaggat gttgtcgcca tcaagcagaa
     5461 tttcaccttc cgcacgctgc tccgggtaca gttcaaacat tttgttgaag gtacgcagca
     5521 gcgtcgattt accgcagccg gacggcccga taaacgccgt aacctggttt ttggcgatat
     5581 ccaggttgat gtttttcagg gcatggaatt tgccgtagta gaagttcaaa ttacgaacct
     5641 gaattttact cggggcagtt tcaaccatac tcattgcaat cttttcctca tcgtacctga
     5701 tgcttttcgc taatcaggct taccgtaggg ccgggtaagg cgttcacgcc gcatccggaa
     5761 aagtgttaac cgtgtttatt cttcgcaaaa acaacgcgcg ccagaatgtt cagcagcagg
     5821 acgcacaggg taatgatcaa taccccggcc caggccagtt gttgccattc cgcaaacggg
     5881 ctcatcgcaa acttaaagat cgtcaccggc aggttggcga tcggctgcat catgtccgtg
     5941 ctccagaact ggttggagag cgcggtaaac agcagcggcg cggtttcacc ggcaatacgg
     6001 gcaatcgcca gcaggatacc ggtcataatc ccggacaccg acgctttcag cgtaatcgcg
     6061 gagatcatct tccacttcgg tgtacccagc gcataagccg cttcacgcag gctgtccggc
     6121 accagtttca gcatgttctc agtggtgcgg ataacgatcg gcacctgcaa cagcgccagc
     6181 gcaattacgc ccgcccagcc ggagaagtgc tccatctgtg ccaccacgat ggtgtagaca
     6241 aacagaccaa ccacaatcga cggcgcggag agcagaatgt cgttaatgaa gcgaatcact
     6301 tctgccagcc aggatttacg accatattcc gccagataaa tccccgccat aatgcccagc
     6361 ggcgtaccga ataccgtggc ccacaaaatt aacagcccgc tacccgccag ggcgttcgcc
     6421 agaccaccac cttccgtatt gggcggcggc gtcatttcag tgaacagcgc cagcgacata
     6481 ccgtcgatac cgcgagtgat ggtggacatt aaaatccaga tcagccagaa caggccgaag
     6541 gccatcgtcg ccatcgagag cgtcagcgca atacggtttt tgaggcggcg acgcgcctgc
     6601 attttgcggc gagattcagc cagcgccgca gtggtttgca tttcaaccat agccattagc
     6661 gtgccccctc attcttagcc aggcgcataa tcataaactt cgatgcggcg aggacgatga
     6721 aggtaatcac aaacaggatc aggcccagtt ccatcagtgc ggcaacgtgc agaccggatt
     6781 ccgcttccgc aaattcgttc gccagcgcag aggtgatact attgcccggc atgtacagcg
     6841 aggcgctgtc gagctggtag gtgttaccga tgataaaggt caccgccatg gtttcaccga
     6901 gcgcgcggcc cagccccagc atgatgccgc cgataacacc atttttggtg aacggaagaa
     6961 cgatacgcca gataacttcc caggtggtgc agccaatacc gtaggccgac tctttcatca
     7021 tcaccggggt ttgttcgaac acatcacgca ttaccgccgc aatgtacgga ataatcatga
     7081 tggcgaggat cacgcctgcc gcgaggatac cgataccaaa tgcggggcca gagaacagcg
     7141 cgccaacaat cgggatattc gacatgatat tgccgaccgg ctcctgaaag taaacggcga
     7201 acagcggcgc aaagataaac aggccccaca tgccgtaaac gatacttgga atggctgcca
     7261 gcagctcaat ggcgataccc agcgggcgtt tcagccagcc aggcgcaagc tcagtcagga
     7321 acagggcgat accgaaactc accgggacgg cgatcagcag cgcgataaac gaagtcacca
     7381 acgtaccgta gatcggcacc agcgccccgt agatatcgtt cggtgcatcc cactctttgg
     7441 tccataggaa agccagacca aatttctgaa tgctcggcca ggaggagatg atcagagaga
     7501 caataatgcc acccaacatc aatagcacaa tcagcgccgc cagttttacc agcacgctga
     7561 aaattatgtc gcccttttta cccggtgggt taaaagcggg cttggttgca gccataagtt
     7621 actcttcagt taaacgcgtt tacaaagtca ttttgtaatg ccggatgcgg cgtaaaacac
     7681 cgtacccggc ctggagtttt attagtacag cggcttaccg ctactgtctt taatattggt
     7741 cttccacgca gcgcgaacct gttcaactac actatccggc aggctggcgt aatccaggtc
     7801 gttcgcctgt ttagccccgg ttttgtacgc ccagtcgaag aatttcagca cctctgtgcc
     7861 ttgttctggt ttcttctgat ctttgtggat cagaatgaac gtggtagagg taataggcca
     7921 tgcatcttcg cctttctggt tggtcagatc ctgagcgaag gttttgctcc agtctgcacc
     7981 ttttgctgca ttagcgaagt tttcttcggt cggactaacc ggtttaccat cagcggagat
     8041 cagtttggtg tacgccaggt tgttctgctt cgcgtaagca tattcaacat aaccaattgc
     8101 acccggcaga cgctgaacga acgcggcgat accgtcgtta cctttaccgc ccagaccgat
     8161 cggccatttt acggtagagc cagtaccaac gttgtttttc cactcttcgt tcactttcgc
     8221 caggtagctg gtgaagacga aggaagtccc ggagccatct gcgcggcgta ctacagcaat
     8281 gttttgtgaa ggcagtttca gacccggatt cagtttggcg atggcttcat catcccactt
     8341 cttgattttg cccaggtaga tgtcgccgag ggttttacca tccagcacca gttcgccaga
     8401 cttcagccct ggaatgttaa ccgccagcac cacgccgcca atcacggtcg ggaactggaa
     8461 cagaccttcc tgagccagtt tttcgtcaga cagcggcgcg tcagaggcac caaaatcaac
     8521 ggtattagcg ataatctgtt ttacgccacc ggaagaaccg ataccctggt agttaacttt
     8581 attaccggtt tctttctggt aagtgtcagc ccatttggca tacaccggcg cagggaaggt
     8641 tgcacctgca cctgtcaggc ttgcttctgc aaacacagag aaagcactca tcgataaggt
     8701 cgcggcgaca acagttgcga cggtggtacg cataactttc ataatgtctc ctgggaggat
     8761 tcataaagca ttgtttgttg gctacgagaa gcaaaatagg acaaacaggt gacagttata
     8821 tataaggaat atgacatttt tatgacagaa aggaattacc aaataactta ctgattttaa
     8881 atgtgaagat gaaaattcca catgccatct ttttgtcatg atgcatttaa aagtgataaa
     8941 gaaatgggtg agatgacaac gatgtgatga acaatcgttg tcagacctat tagcgcacaa
     9001 tctcaacagt tatcgtcgct gttgcagtaa attgccccgg ggctgggcga gctccagtaa
     9061 ggctcgcagg agcagctgaa aaagttacac tacctgattt ttcaccatac actgttgatt
     9121 tatccatgtc tgttggcaac tcaccaccgt tcactttgat ttccttatta tttttgtcgt
     9181 aaatttttat ccccacgtta gagtttgaag ttacgaccat tgaattgtcc gtattgggtt
     9241 cggtagcata aaaagacgta ctgatatcat cggtattcag tatccccagg ttttcacact
     9301 caatagtgac tgttttagta attttatgtg ttgttaccgc acttccaaca acagatgaaa
     9361 aatctgcagc tggtatttct ttaaaatcaa aacaaactgg ctttaaatta tttatttcgc
     9421 aactttgcgg agctgtaata tcgccccgaa tataaacatc agccatcggc tgtgagtaat
     9481 ccaccggggt gtcacggcta attgttccat acaatgtcac tatctttgtt tcaggaataa
     9541 tcaattttcc tgcaaatgtc ttcttgatat aaaaatatac tatcgcttta gccccactgg
     9601 atatacttaa aggttcaatg cggttgcaat tgtactttgt attaccagac gggttgttag
     9661 catgatattc aaaaggtgca ttcacatacc caaccccaag aatatctata gcaatcccaa
     9721 catcaaggtt cggattaaga tcataataat taatattaga gtgaacccca gcataagtta
     9781 atgcctggtt tatttttgac gtataataga tataggggaa ttctcctacg ttaccagtat
     9841 cacaatgaca gcgtccaaga tatgaggatc cattagttac agtggtgtta aatgtcgttc
     9901 caaccttatt ttcgttcgcg gtaataagat atccc
//
LOCUS       DAJTSI010000140         9917 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000140 DAJTSI010000000
VERSION     DAJTSI010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9917
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.140"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            326..1321
                     /gene="pgl"
                     /locus_tag="OO327_003249"
     CDS             326..1321
                     /gene="pgl"
                     /locus_tag="OO327_003249"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR6019680.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1362..2315)
                     /locus_tag="OO327_003250"
                     /pseudo
     CDS             complement(1362..2315)
                     /locus_tag="OO327_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2499..3551
                     /locus_tag="OO327_003251"
     CDS             2499..3551
                     /locus_tag="OO327_003251"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCR6019681.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            3627..5060
                     /locus_tag="OO327_003252"
     CDS             3627..5060
                     /locus_tag="OO327_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCR6019682.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5243..7423
                     /locus_tag="OO327_003253"
     CDS             5243..7423
                     /locus_tag="OO327_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR6019683.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(7564..8847)
                     /locus_tag="OO327_003254"
     CDS             complement(7564..8847)
                     /locus_tag="OO327_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR6019684.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(8982..9863)
                     /locus_tag="OO327_003255"
     CDS             complement(8982..9863)
                     /locus_tag="OO327_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6019685.1"
                     /translation="MTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMSKIKAI
                     RRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTNPV
                     AATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCEMKWSD
                     IVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLS
                     SGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMA
                     SQYRDDRGREWDKIEIK"
BASE COUNT         2400 a         2317 c         2518 g         2682 t
ORIGIN      
        1 atttgtgccg aaagtgagtt gattgttgcg cgaatgggaa gttggccaga agttgttatg
       61 gcatagtgtt tacggtcgtt gatacaggta aacaaaagcg gggagagtca tactccctga
      121 atagtcgcga aaatatctgt ggcattgtca aattcgtgtc ggtgactaat attttagtaa
      181 tatgccattc gaaatggagt gctaacttat tgatttaatt gatgtgcgat attgtatttt
      241 atcgtgtttt gttgtctgtt ttttatccag taagtgttgt tatatgtatg aaagataagg
      301 gatttatcat ttttgaggtg tgttcatgaa gcaaacagtt tatatcgcca gccctgagag
      361 ccagcaaatt cacgtctgga acctgaatca tgaaggcgca ctgacgctga cacaggttgt
      421 cgatgtgccg gggcaggtgc agccgatggt ggtcagcccg gacaaacgtt atctctatgt
      481 tggtgttcgc cctgagtttc gcgtcctggc gtatcgtatt gccccggacg atggcgcact
      541 gacctttgcc gcagagtctg tgctgccggg tagtccgacg catatttcca ccgatcacca
      601 ggggcagttt gtctttgtag gttcttacaa tgcgggtaac gtgagcgtaa cgcgtctgga
      661 agatggcctg ccagtgggcg tcgtcgatgt agtcgagggg ctggacggtt gccattccgc
      721 caatatctca ccggacaacc gtacgctgtg ggttccggca ttaaagcagg atcgcatttg
      781 cctgtttacg gtcagcgatg atggtcatct ggtggcgcag gaccctgcgg aagtgactac
      841 cgttgaaggg gccggcccgc gtcatatggt attccatcca aacgaacaat atgcgtattg
      901 cgtcaatgag ttaaacagct cagtggatgt ctgggaactg aaagatccgc acggcaatat
      961 cgaatgtgtc cagacgctgg atatgatgcc ggaaaacttc tccgacaccc gttgggcggc
     1021 tgatattcat atcaccccgg atggtcgcca tttatacgcc tgcgaccgta ccgccagtct
     1081 gattaccgtt ttcagcgttt cggaagatgg cagcgtgttg agtaaagaag gcttccagcc
     1141 aacggaaacc cagccgcgcg gcttcaatgt tgattacagc ggcaagtatc tgattgccgc
     1201 tgggcaaaaa tctcaccaca tctcggtata cgaaattgtt ggcgagcagg ggctactgca
     1261 tgaaaaaggc cgctatgcgg tcgggcaggg accaatgtgg gtggtggtta acgcacacta
     1321 accgctgatt taaccggcgc agtctctcct gcgccggtgt attaacttat ctcctgtaac
     1381 gcatgtctct ggcgttcgac gatattggtc cacaaattgt ctttatcgtc agtccacaaa
     1441 ttaatcagca aggaaaaaaa gcgttcagct gccggagaaa gcacggcatc tttgcggcga
     1501 ataatcccca atgtccgacg tataacgggt tccaccagtg ggataccaat aatcgaagaa
     1561 tagggcgcat ggggcattgc cagaccagga agcgccgaaa tccccagtcc tgcctccact
     1621 agtcctaatg acgtcgaaag atgacgcact tcgtaaaacc agtccagctt ccagggcttg
     1681 tcggctagct gttgttctat caacaatcgg ttgccgctgg aggaacgtac gccaatcatt
     1741 ttgtaaccca ctaactcctg ccattctaca agctactttt tggccaatgg atggtcacgc
     1801 cgacaggcca gcacgaatgg ctcgttgacc agtggggtaa aatcaatgga tgaatttgtg
     1861 acgttgttca tgtttatgcc aaagtctgat tcgttacaca gcaccgattc catgcaatta
     1921 ttcgtgcctt gttccagaat ccgcacttta atattgggat acagctcatt aaatttaccg
     1981 attgccagcg gtaaaaaata aaatactgcg gtcggaatac acgctaatgt caccatacca
     2041 cgatgataag cattcatatc acgaatatta aaaagcgttt catcgaattt ctttattaat
     2101 tcccgcgcct ccgggagcaa cctttttcct gcttttgtta acgtaacctt gcgagttgta
     2161 cgctcaaata gttgaatatg taaatcttcc tccatctttt ttattcttcg cgttaatgca
     2221 ggttgcgtaa tattgagtaa tttagcggca ttgttaaatg aactggactc tgccagtatg
     2281 acaaatgcct tcatacttga taattcatgc tttattatta ctccggaaaa tggaagcgac
     2341 gattttgggg ggctggtcgt taaaaatttt aactgcattt agccaactta aattaatgaa
     2401 aaaatgttat taatcgttga gctaaagtca ttagagatgc tttgtcctta atgtaaccat
     2461 atcgcaataa gttatgtttt taaattgagg gcattattat gaaaaaaata ccctgcgtga
     2521 tgatgcgagg tggaacctcg aggggcgcgt tcctgttagc ggaacattta cccgaagatc
     2581 aaacgcagcg cgataaaata ttgatggcaa ttatgggttc cggtaacgat ctggaaattg
     2641 acggtattgg cggcggtaat ccactgacca gtaaagtcgc cattattagc cgttccagcg
     2701 atccgcgtgc tgatgtcgat tatctgtttg ctcaagttat cgtccatgag caacgtgtcg
     2761 ataccacgcc taactgcggc aatatgctgt ctggcgtagg ggcattcgcc attgaaaatg
     2821 gtttgattgc agcgacttcg ccagttaccc gtgtacgtat ccgcaacgtc aatacgggta
     2881 cgttcatcga agctgatgtg caaacgccaa atggtgttgt cgagtacgag ggtagcgcca
     2941 gaattgacgg cgtaccgggt actgccgcac cggttgcgct cactttcctg aatgccgctg
     3001 gaacgaaaac tggaaaagtt ttcccgactg ataatcagat tgattatttt gacgatgtcc
     3061 cggtgacctg tatcgatatg gcgatgccag tcgtcattat tccggctgaa tatctgggaa
     3121 aaacaggtta tgaattaccg gcggaactgg atgccgacaa agcattatta gcccgcattg
     3181 aatctatccg gctacaagcg ggtaaagcaa tgggcttagg tgatgtcagt aatatggtta
     3241 tccctaaacc tgtgcttatt tctccagcgc agaaaggcgg ggcaattaat gtgcgttatt
     3301 ttatgccgca ttcttgccat cgcgcgctgg cgataaccgg tgctattgct atttccagta
     3361 gttgtgcatt ggaaggcacc gtcacccgac aaatcgtccc ttctgtagga tatggcaata
     3421 tcaatattga acaccccagt ggtgcgctcg acgttcattt aagtaatgaa ggtcaggatg
     3481 ccacgacgtt acgcgcatct gttattcgga caaccagaaa aatattttcc ggtgaagttt
     3541 atcttccctg aaaaaattcg ttgtcaggat aaggacaatc aataaaggac ttctgtatga
     3601 gtcatacaga aagaacagga ttttaaatga ataagaaatc gttatggaag ctaattctga
     3661 tattagcgat cccatgtatt attggtttta tgccagctcc ggcaggatta agcgaactgg
     3721 cgtgggtgct ttttggtatt tacctggcgg ccattgtggg tctggttatc aagcctttcc
     3781 cggaacctgt cgtactgtta attgccgttg ctgcctcaat ggtggtggtc ggtaacttat
     3841 ccgacggtgc gtttaagacc accgccgtat taagcggtta ctcttcaggt actacctggc
     3901 tggtgttctc ggcgtttacc ttaagcgccg catttgtgac caccggttta ggtaaactta
     3961 ttgcctatct gctgattggt aaaatcggta acaccatgct gggtctgggt tacgttacgg
     4021 tattcctcga tctggtactg gctccggcaa caccgtctaa caccgcgcgt gcgggcggca
     4081 ttgtgttacc gatcatcaac agcgtggcgg tggctttggg gtccgaaccg gaaaaaagtc
     4141 cgcgtcgtgt cggacattac ctgatgatgt ccatttacat ggtcaccaaa accaccagct
     4201 atatgttctt taccgcaatg gcggggaaca ttctggcgct gaaaatgatc aacgacattc
     4261 tgcacctgca aattagctgg ggtggatggg cgctggccgc cggattgccg ggcatcatta
     4321 tgctgctggt caccccgctg gtaatttaca ccatgtatcc accagaaatt aaaaaggtgg
     4381 ataacaaaac catcgctaaa gcgggccttg ccgaactggg accgatgaaa atccgcgaaa
     4441 aaatgctgct cggtgtcttt gtgctggcgc tactgggctg gattttcagt aagtctctgg
     4501 gggttgatga atccactgtg gcaatcgttg ttatggcgac catgctgctg ctgggtatcg
     4561 ttacctggga agacgtggtt aaaaataaag gcggctggaa taccttaatc tggtacggcg
     4621 gtattatcgg cttaagctcc ttattatcga aagttaaatt tttcgaatgg ttagctgaag
     4681 tctttaaaaa taacctggca tttgatggtc acggtaacgt tgctttcttc gttattattt
     4741 tcctcagcat catcgtgcgt tatttcttcg cttccggtag tgcctatatc gttgctatgt
     4801 tacctgtatt tgccatgctg gcgaacgtct ccggcgcacc gttaatgtta accgcgctgg
     4861 cactgttgtt ctccaactcc tatggcggca tggttactca ctatggcggc gcggcaggtc
     4921 cggtcatctt tggcgtgggt tacaacgata ttaaatcctg gtggttggtc ggtgcggtac
     4981 tgacgatatt aaccttcttg gtgcatatca ccctcggcgt gtggtggtgg aatatgctga
     5041 tcggctggaa catgctgtaa atatactcgt catacttcaa gttgcatgtg ctgcgtctgc
     5101 gttcgctcac cccagtcact tacttatgta agctcctggg gattcactca cttgtcgcct
     5161 tcctgcaact cgaattattt agagtatatc catttattat ctttctgcgc acttcacggt
     5221 gcgcagatat ctggagcatt tgatgatcaa gttatctgaa aaaggcgtgt ttctcgccag
     5281 taataacgaa ataattgccg aagaacattt caccggcgaa attaaaaaag aagaagcaaa
     5341 aaaaggcact attgcctggt ctattctctc ttctcataat acgtccggaa atatggataa
     5401 acttaaaatt aagtttgatt cattagcctc tcacgatatt acctttgttg gtattgtaca
     5461 gaccgctaaa gcgtccggaa tggaacgttt cccgctgccg tatgtgctga ccaactgcca
     5521 taactcactc tgcgccgtcg gcggcaccat taacggtgat gaccatgttt ttggtttatc
     5581 ggcagctcag cgttatggcg gtatttttgt gcctccgcat attgcggtca tccatcaata
     5641 tatgcgtgag atgatggctg gcgggggcaa aatgatcctc gggtcagaca gccacacccg
     5701 ttacggtgca ttagggacaa tggcagtcgg tgagggtggc ggtgagttgg taaaacagct
     5761 gcttaatgac acctgggata tcgactatcc gggcgtggtt gcggtgcatc tgaccggaaa
     5821 acctgcgccg tatgtggggc cacaggatgt ggcgctggct atcattggcg cggtgttcaa
     5881 aaacggttac gtcaaaaaca aagtcatgga gttcgttgga ccgggcgtta gcgcgctctc
     5941 taccgatttc cgtaacagcg ttgacgtgat gaccacggaa accacctgtt taagctccgt
     6001 ctggcaaact gatgaagaag tgcataactg gctggcgctg cacggtcgcg gtcaggatta
     6061 ctgccagctt aaccctcaac cgatggcgta ctacgatggc tgcatcagcg ttgatttaag
     6121 cgccatcaaa ccaatgattg cgctgccgtt ccacccgagc aacgtgtatg aaatcgacac
     6181 actgaaccag aacctgaccg acattctgcg tgagattgaa attgagtccg aacgcgtggc
     6241 gcacggtaaa gccaaactct cgctgctgga taaagtggaa aatggtcgcc tgaaagtgca
     6301 gcaggggatt atcgcgggct gttctggcgg taactacgaa aacgtcatcg cggcggcgaa
     6361 tgcactgcgc ggtcaatcct gtggcaatga caccttctcg ctggcggttt acccgtcatc
     6421 acagccggtg tttatggatc tcgccaaaaa aggtgtggta gcagatttga ttggcgcagg
     6481 cgcaatcatc agaaccgcat tctgtggccc atgctttggc gcgggcgata cgccaatcaa
     6541 caacggtttg agtattcgcc acaccacgcg taacttcccg aaccgcgaag gctctaagcc
     6601 agccaatggg cagatgtcag cggtggcgtt gatggacgct cgttctatcg ctgcgactgc
     6661 ggcaaacggt ggctatttaa cctctgccag cgaacttgat tgctgggaca acgtgccgga
     6721 gtacgccttc gatgtaacgc cgtataaaaa ccgtgtttat cagggctttg tgaaaggggc
     6781 aactcagcaa ccgctgattt acggaccgaa cattaaagac tggccggaat tgggtgcgct
     6841 gactgacaat atcgtcctga aagtgtgctc gaagatcctc gacgaagtca ccaccaccga
     6901 cgaactgatt ccttccggtg aaacctcttc ttatcgttca aatccgattg gtctggcggg
     6961 gaatgtcagc gagctgacag aggtgtttgc gcgcattaag cagattgctg gtcaggagca
     7021 tattgatccg ctgcaaactg aaattggcag catggtctat gcggtgaaac caggcgatgg
     7081 ttctgcgcgt gaacaggcgg cgagctgcca gcgtgtgatt ggcggtctgg cgaatattgc
     7141 cgaggagtac gcgactaaac gctaccgttc taacgtcatc aactggggga tgttaccgct
     7201 gcagatggcg gaagtgccaa cctttgaagt gggggattac atttacatcc ctggcattaa
     7261 agcggcgctg gataatccgg gtacgacgtt taaaggttat gtgatccatg aagatgcgcc
     7321 ggtaacggaa attacgcttt acatggaaag cctgacggct gaagagcgcg agattatcaa
     7381 ggcgggtagt ttgattaact tcaataaaaa ccgtcagatg taaaaagcgc catgtgaatg
     7441 taggtcgcat tcggcactta ttgtcggatg cgatgctggc gcatcttatc cgacctacaa
     7501 atcgcatcga accgtaggcc ggataaggcg tttacgccgc atccggcaaa tagttaatag
     7561 ctcttacttc ttctcctctg caaccacttt gctgcccacg ccgcggttat tgtattccca
     7621 catgcggttg tagttagtgt cattcagatt gcgctgtatt tcgtcgttat catctacgct
     7681 gccggtatta cccgcaaacg gacgattaga gatcaccgca tcggcccacg gtttagccgt
     7741 gttaaaacct tcgttgatgg cgctatcacg gatcaccacc tgaccgttgg tattagcatc
     7801 aacatccagc gagcggccca gttgcgccac gccatcaccg gaagcattga aacggctgtt
     7861 tacggcgagg aaaccgtaat agatgttaga cagcgtagcc ggtgcaaaca catatgcttc
     7921 ttgctgggta cgtgagttca ccacgcggaa ttcggtgtta tcgaacacca ctgcgccgcg
     7981 accagaaacg atatccacat ccccttcaat atagctgttg gtcaccagcg tacgcggctg
     8041 acggttggtt tccagacggt tctgcacacc gctgttggtg acaaagaagg tgttctgacg
     8101 accgagaatg ttaacgttgt taatctgtac ttggtcaccg tcagtacgca gtgctaccgc
     8161 cggatggtta cctgcatcta cgctatcgcc cagcgtgttt tcgatggtca gattttgcag
     8221 ttgcagtcca ttgttttgtg accagaagac cgcagaacac agaacaccga tactgtcgct
     8281 gcgtttgctc tggcagctat cgtacatata ccacgctggt ttacctggca tatatttgcc
     8341 gcgcgggttg acgtcgtgac gccagtcggc agggctcatg cccccatcaa gggaaagccc
     8401 aatcttcaca tcaatcggtt tttcacctgt accgtacaga gtgattccac ctggagcagc
     8461 agggacatac accgttccct gatactcacc aggcatcacg gcaatatact ggcgcttgtt
     8521 ggtgcgcttg ataattgccg catctaccgc cgcctgaatc gtggtatgcg ttacaccttg
     8581 agtacccgcc gggccgacaa caaagtcagg ttgcgcaggc agggttatcg gggacggatt
     8641 ccacgctgca gcacctggtg tcagggatgc aaaatagtgt tgcgcatcga aattcagcgc
     8701 ttcttttgcc gacagaatcg ggcgagaaga ggtaccaggc gcagtttgat cagaaggacg
     8761 ttgatcgggt ggtgttgagc tacaggcggt cagcgtcacg ccaaaagcca atgccagcgc
     8821 cagacgggaa actgaaaatg tgttcacagg ttgctccggg ctatgaaata gaaaaatgaa
     8881 tccgttgaag cctgcttttt tatactaagt tggcattata aaaaagcatt gcttatcaat
     8941 ttgttgcaac gaacaggtca ttatcagtca aaataaaatc attatttgat ttcaattttg
     9001 tcccactccc tgcctctgtc atcacgatac tgtgatgcca tggtgtccga cttgtgcccg
     9061 agaagatgtt gagcaaactt atcgcttatc tgcttctcat agagtcttgc agacaaactg
     9121 cgcaactcgt gaaaggtagg tggatcccct tcgaaggaaa gacctgatgc ttttcgtgcg
     9181 cgcataaaat accttgatac tgtgccggat gaaagcggtt cacgacgagt agatgcaatt
     9241 atggtttctc cgccaagaat ctctttgcat ttatcaagtg tttccttcat tgatatcccg
     9301 agagcatcaa catgcaatgt tgtagggatg gcaattttta cgcctgtttt gctttgctcg
     9361 acataaagat atccatctac gatatcagac cacttcattt cgcataaatc accaactcgt
     9421 tgcccggtaa caacagccag ttccattgca agtctgagcc aacatggtga tgattctgct
     9481 gcttgataaa ttttcaggta ttcgtcagcc gtaagtcttg atctccttac ctctgatttt
     9541 gctgcgcgag tggcagcgac cgggtttgtt gttatatggc cttcagctat tgcctctcgg
     9601 aatgcatcgc tcagtgttga tctgattaac ttggctgatg ccgccttgcc ctcgtctatg
     9661 tatccattga gcattgccgc aatttctttt gtggtgatgt cttcaagtgg agcatcaggc
     9721 agccccctcc ttattgcttt aattttgctc atgtaattta tgagtgtctt ctgcttgatt
     9781 cctctgctgg cgaggatttt ttcgtagcga tcaagccatg aatgtaacgt aacagaatta
     9841 tcactgttga ttctcgctgt cagaggcttg tgtttgtgtc ctgaaaataa ctcaatgttg
     9901 gcctgtatag cttcagt
//
LOCUS       DAJTSI010000141         9905 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000141 DAJTSI010000000
VERSION     DAJTSI010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9905
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.141"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(160..900)
                     /gene="pflA"
                     /locus_tag="OO327_003256"
     CDS             complement(160..900)
                     /gene="pflA"
                     /locus_tag="OO327_003256"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR6019686.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(1092..3374)
                     /gene="pflB"
                     /locus_tag="OO327_003257"
     CDS             complement(1092..3374)
                     /gene="pflB"
                     /locus_tag="OO327_003257"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR6019687.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3429..4286)
                     /gene="focA"
                     /locus_tag="OO327_003258"
     CDS             complement(3429..4286)
                     /gene="focA"
                     /locus_tag="OO327_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR6019688.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4692..6452)
                     /locus_tag="OO327_003259"
     CDS             complement(4692..6452)
                     /locus_tag="OO327_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR6019689.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6582..7208
                     /locus_tag="OO327_003260"
                     /pseudo
     CDS             6582..7208
                     /locus_tag="OO327_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            7407..8495
                     /gene="serC"
                     /locus_tag="OO327_003261"
     CDS             7407..8495
                     /gene="serC"
                     /locus_tag="OO327_003261"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR6019690.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8566..9849
                     /gene="aroA"
                     /locus_tag="OO327_003262"
     CDS             8566..9849
                     /gene="aroA"
                     /locus_tag="OO327_003262"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR6019691.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         2520 a         2368 c         2483 g         2534 t
ORIGIN      
        1 ttcgatgggg aagaacttca cgcgagctat tatcagtcgt tattatctca ggtacgggat
       61 atggtagatg cactataaga tgtgttaaaa acgctgtagc agaatgaagc gcggaataaa
      121 aaagcggcaa ctcaataaag ttgccgcttt acgaggaaat tagaacatta ccttatgacc
      181 gtactgctca agaatgcctt tcacgcgttc catggtctct ttcttcggtg gtttaacacc
      241 atcgagtttg tattcttcac ccatcgccac ccatttgtgt ttgcccagtt cgtggtaggg
      301 gagaagttcg attttctcaa cgttacccat atcacgggta aattcaccga ggcgatgtgc
      361 cgaatcgtca tcgtcagacc agcctgggac aacaacgtag cggatccaca ccttcacatt
      421 tttgttcgcc agatatttag cgaactccag cgtgcggtgg ttggaaactc caaccagatt
      481 ttggtggatc tcgtcgttca tctgtttgag atcgagcatt accaggtcgg ttacttccag
      541 cagttcatca atcaccggat cgtaacgacg aacaaaaccg ttggtatcca gacaggtatg
      601 aatgccttct tttttacagg cgcggaacca gtcacgaaca aactcagctt gcaggattgc
      661 ttcaccgccg gatgcggtaa cgccgccgcc ggaagcgttc ataaagtggc gataggtcac
      721 cacttccttc atcaaatctt caacggtaac ttctttaccg ccatgcgtgt cccaggtgtc
      781 gcggttatga caatacaggc agcgcatcag gcagccctgg aaaaaggtga taaagcgaat
      841 acctgggccg tctacggttc cacaggattc aaaggagtga atgcgaccaa taactgacat
      901 tgcggtgttt ctccagatgt ggcccatctg aggccgtgtt ggtgcgcagc tcgaaggcta
      961 cgtcgagtct gttttggcag tcaccttaaa gtatagatag ctgacaaaaa aggctctcgt
     1021 gctaaaaaag gccccacttt cgtggagcct ttattgtacg ctttttactg tacgatttca
     1081 gtcaaatcta attacataga ttgagtgaag gtacgagtaa taacgtcctg ctgctgttct
     1141 ttagtcagcg agttgaaacg tactgcgtag ccagatacac ggatggtcag ctgcggatat
     1201 ttttccgggt tttccatcgc gtcgagcagc atttcacggt tcatcacgtt aacgttcagg
     1261 tgctgaccac cttcgatgga tgcttcgtgg tggaagtaac catccatcag accagccagg
     1321 ttggtcttac gaacttcgtc gtctttaccc agtgcgttcg gaacgataga gaaggtgtag
     1381 gagataccat ctttagcgta agcaaacggc agtttagcaa cggaagtcag agacgctaca
     1441 gcacctttct ggtcacgacc gtgcatcggg ttagcacccg gtccgaacgg cgcgccagca
     1501 cgacgaccgt ctggggtgtt accagttttc ttaccataca caacgttaga agtgatggtc
     1561 agaacagact gagtcgggat agcgtcacgg taggtgtgca gtttctgaat tttcttcatg
     1621 aaacgttcta ccaggtcaac agccaggtca tctacacgcg gatcattgtt accaaactgc
     1681 gggtattcgc cttcgatttc gaagtcgata gccagaccgt cttcgtcacg aatcggttta
     1741 actttcgcat atttgattgc agacagggag tcagcagcaa cggacagacc agcgatacca
     1801 cacgccatgg tgcggataac gtcacggtcg tgcagcgcca tcagagaggc ttcgtagctg
     1861 tacttgtcgt gcatgtagtg gatgatgttc agtgcagtga tgtactgttt agccagccag
     1921 tccatgaagt gatccatgcg ctccatcact tcatcgtagc tcaggagatc gcctttgatc
     1981 ggttcagact tcggaccaac ctgcattttc agtttttcgt caacgccgcc gttgattgcg
     2041 tacagcatgg ttttcgccag gtttgcacgc gcaccgaaga actgcatttg tttaccaacg
     2101 atcatcgggc ttacgcagca agcgatagcg tagtcatcgt tgttgaagtc cggacgcatc
     2161 aggtcatcgt tctcatactg cagagaagag gtgtcgatgg acactttagc ggcgaatttc
     2221 ttgaagttca gcggcagttt ttcagaccac agaatggtca tgttcggttc cggagacggc
     2281 cccatagtgt acagggtgtt caggaaacgg aagctgtttt tggtaaccag ggtacgacca
     2341 tcgaggccca taccaccgat agattcggtt gcccagattg ggtcgccaga gaacagttca
     2401 tcgtattccg gagtacgcag gaagcgaacc atacgcagtt tcatgaccag gtggtcaacc
     2461 atttcctgcg cttcttgttc ggtgatcttg ccagctttca ggtcacgttc gatgtacaca
     2521 tccaggaagg tggaggtacg accgaaggac attgcagcac cgttctgaga cttaacagca
     2581 gccaggtagc cgaagtaagt ccactggata gcttcctgag cgttggtagc cggaccagag
     2641 atgtcgtagc cgtatttcgc agccatttct ttcatctgac ccagagcgcg gtgctgttca
     2701 gcgatttctt cgcgcagacg gatagtctgt tccaggttta cgccgttttc cagatcagcc
     2761 tgtagagagg tgaactgagc gtatttgtct ttcatcaggt agtcgatacc gtacagcgca
     2821 acgcgacggt agtcaccgat gatacggcca cggccataag catctggcag accggtcaga
     2881 acaccggatt tacggcaacg caggatgtcc ggagtgtaaa cgtcgaacac gccctggttg
     2941 tgagttttac ggtattcagt gaagattttt ttgatcatcg ggtccagttc gcggttgtac
     3001 gctttgcagg aaccttcgat cattttgata ccaccgaacg ggataagagc acgtttcagc
     3061 ggagcttcag tctgcagacc aacaactttt tccaacgctt tattgatgta gccagcgtcg
     3121 tgagaggtga tggtggaagc aacagcggtg tcaaaatcaa ctggcgcgtg agtgcggttt
     3181 tccagtttaa caccttccat tactttgtcc cacagggtgg tggtcgcttc agtagcgcca
     3241 gccaggaagg actcgtcacc ctcgtacgga gtgtagtttt tctgaatgaa gtcacggacg
     3301 tttacttcat tctgccagtc acctttggta aaaccttccc aggctgtggc taacttttca
     3361 ttaagctcgg acatgtaaca cctaccttct taagtggatt ttttatttac tgcgtacttc
     3421 gacaaccatt aatggtgatc gttttcacgc aggtaaatga cccagtatgt caacccaacc
     3481 aacaaaccac cgccgataat attaccgatc gtaaccggga tcaggttatc agtgatgaag
     3541 ttcatcacgg tcaggtgaga aaaattttcc ggtgcagaac cgacagcggt ccagaattcc
     3601 ggagatgcga agtcgcggat tacaataccc atcgggatca taaacatgtt tgcgatactg
     3661 tgctcaaaac cgctggcaac aaacatcgcg accggcagca ccataatgaa cgctttgtcc
     3721 atcaggctgc ggccagaata actcatccat actgccagac ataccatcag gtttgccagg
     3781 ataccaagac agacggcctc aataaaagta tggtgcactt tgtggtcggc ggtttgtagg
     3841 acgtttagtc cccattgacc atttgcggtc atatactcgc cggaaagcca cattaaaagt
     3901 acaaacagca gtgcgccgac caggttgcca aaatagacat ttagccagtt tttagccaac
     3961 tgacaccagg tgatgcgccc actcgcctta gcaacaacaa tcaacactgt ggaagtaaag
     4021 agatcggctc cgcagacaac acaaagaatc agccccagag agaagcaaat gccgccaacc
     4081 agttttgcca tgccgaaggg cattgtgcct gtgccagtgg ttgctgtgat atagaagacg
     4141 aatgcgattg agatgaaaac accggcggta atcgccagat agaaagtctt aagcggatgt
     4201 ttcgttgctt tatagacacc cgcctcttcg gccactttgg ccattgcagc aggaagtaaa
     4261 agatcaaaag ggttgtcagc tttcacacta actctctctt tattaagtcg gcgacgagat
     4321 actaacaaag cattatagat gagaaattga tatagatcat atctcgcctg gcttataggc
     4381 ccgtaactcg catggttttt atgcaaatac ggagtaacta tttgattatc caaataaaaa
     4441 taaattttaa aaattaacaa atgagttgaa ttttttccgc atcctccgct aaaacagtta
     4501 attaaaaggg agcatcaggc gaataaagta acaatatcga tcgtatttat taaatacaaa
     4561 ttaccgatat ttaaccttat aattacaatt attttattaa tgcaaatata tgtaaagcgg
     4621 ggcattaaaa aaacgccccg taatataact cagactaatc attaagccta cattgcgtag
     4681 gctatttgat tttattttgc ccagaatgct gctttggcgc gctgcagctt ttcgtaggcc
     4741 ttcaacaacg actgatgtgc agcaaacgcg tgcagatcgc tatctaccgg ttgcaggccg
     4801 taaaacgccg cttcgccgct cattgccgca ctggcggctt ctacggcatc tgcgccgtac
     4861 atgcgaacaa acgcattcag atattgcagc ggctggcgat cttcttcctg tgccagtaat
     4921 aacaacgttt gcaggcagcg ataatagttg gcgcgttccg gactaaatac tgatgagtta
     4981 aactccatcg tccattcggt ccagaccaga gcctgttcca gatcgccacc agccagcgcc
     5041 agcatggctt ttaattcacc gatacgcagg gtgtaccaac cgttatccga cccggtcgcc
     5101 agacccaaca gctcacgcac gcgggtaaag tcatcaaaac cttcttcatc cagttgctcg
     5161 atgaggttca ggtaatcttc tttttcccac tcgctgcctg gtagcgaaag aatcgtttca
     5221 cgtaaatggc tgcccatact gttattcgcg agccacagat cttcagccgg ataaatatcg
     5281 gacatgccag gcacgataat acggcaagca taaacgccca gatgctcgta atcggcaata
     5341 taaacttctt tatcttcttt gttgaagata gccatcagcg tagcaaactc ttcttccgtg
     5401 gtgccggaga aattccagtc cacaaacgga taatcggcat cctgcttgaa caggtcccag
     5461 gagattaaac cgctggaatc gataaagtgc gtttcgaggt tggtatgttc agcgacttct
     5521 tcatcatcga aggttggcgg agtaaacaca tccaaatctt tcaggccacg accttgcagc
     5581 agctcggtca cggtacgttc cagtgctacg ccaaaatcag gatgcgcacc gaaagaggca
     5641 aagcaggtgc cgtttgcagg attaaacagt accacgcaaa tcaccggata ctggccgcca
     5701 agggaaccat catatgcgaa gatcgggaaa ccttctgttt ccagtgtttc gatcgcttca
     5761 accaccgctg ggtaacgcgc cagcacgtct gccgggattt ccggcaagct gatgctttca
     5821 gcaataatgc ggtttttcac gtagcgttcg aaaacttcgg acaacccctg aacgcgtgct
     5881 tcgttgcggg tattgccagc ggacataccg ttagaaacgt acaggttacc aatgatattc
     5941 atcggaatat aaacggtctg attgtcggac tggcgcgtaa acggcagacc gcaaatacca
     6001 cgatcttcgt taccggattg taggtcaatc agcatactgc cggtcaattc attctccgga
     6061 tcgtaaaatg cgcgcagacg gtcatcgagc agcccttctg gcacatcgtc attttcgctc
     6121 agtgggaacc atttttcgtt gggataatgc acaaatggac cgttggcgat ggtttcgccc
     6181 agccagaaat cagcaaaaaa gtagttggtt gagagacgct cgaaatattc accgagtgca
     6241 gaagccagcg ccgctttctt ggttgcgcct ttaccgttgg taaaacacag tgcgcactct
     6301 ttgtcgcgaa tatgtacaga ccagacgtta ggcacgggat tcagccagga ggcctcttca
     6361 atctgaaagc cgaggtctga aagtttttgc tggaagcgag cgatggaatc ttccagagcg
     6421 gcatctttgc cggggataaa tgtttgcgtc atgaaaatca ctttagtcgt acggaaagcg
     6481 cgcaataata cgggttttat ctcaaaggcg ctatcaccgc cgccatgccg atgaactgtt
     6541 gactatgctt ttagcggata aaccacgtta agagcataaa aatgaaggcg ttcgatctcc
     6601 accgtatggc atttgataaa gtgccttttg atttccttgg cgaagttgca ctacgtagtc
     6661 tttatacctt tgtactggtt ttttgttcct caaaatgacc ggaagacgcg gtgtgcgaca
     6721 gatgtcgctg tttgaagtgt taatcattct cacgctgggt tcagcggcgg gagatgtggc
     6781 gttttatgat gatgtgccga tggtgccggt acttatcgtc tttatcactc tggcgttgtt
     6841 ataccgcctg gtaatgtgat ggcgagctgg cctggtcgaa actcaataac tccaacatga
     6901 cggaatttga gttctttatg gagctacgat tgcgtggcgt ggagcagctg gggcaggtac
     6961 gtctggcgat tctcgaaacc aacgggcaaa tcagtgtcta tttctttgaa gatgacaagg
     7021 tgaaaccggg tttacttatt ttacccagtg attgtacgca gcgttacaaa gtggtgccgg
     7081 agtcggcgga ctatgcctgc attcgttgta gtgaaatcat tcatatgaac gcgggggaaa
     7141 aacaattatg tccgcgctgt gcaaatccag aatggacgaa ggcaagtcgg gcaaaacggg
     7201 tgacctgaca gtaaaaacat cggctttttg ctaataatcc gagagattct tttgtgtgat
     7261 gcaagccaca tttttgccct caacggtttt actcattgcg atgtgtgtca ctgaatgata
     7321 aaaccgatag ccacaggaat aatgtattac ctgtggtcgc aatcgattga ccgcgggtta
     7381 atagcaacgc aacgtggtga ggggaaatgg ctcaaatctt caattttagt tctggtccgg
     7441 caatgctacc ggcagaggtg cttaaacagg ctcaacagga actgcgcgac tggaacggtc
     7501 ttggtacgtc ggtgatggaa gtgagtcacc gtggcaaaga gttcattcag gttgcagagg
     7561 aagccgagaa ggattttcgc gatcttctta atgtcccctc caactacaag gtattattct
     7621 gccatggcgg tggtcgcggt cagtttgctg cggtaccgct gaatattctc ggtgataaaa
     7681 ccaccgcaga ttatgttgat gccggttact gggcggcaag tgccattaaa gaagcgaaaa
     7741 aatactgcac gcctaatgtc tttgacgcca aagtgactgt tgatggtctg cgcgcggtta
     7801 agccaatgcg tgaatggcaa ctctctgata atgcggctta tatgcattac tgcccgaatg
     7861 aaaccatcga cggtatcgcc atcgacgaga cgccagactt cggcaaagat gtggtggtcg
     7921 ccgccgactt ctcttcaacc attctttccc gtccgattga cgtcagccgt tatggtgtaa
     7981 tttacgctgg cgcgcagaaa aatatcggcc cggctggcct gacaatcgtc atcgttcgtg
     8041 aagatttgct gggcaaagcg aatatcgcgt gtccgtcgat tctggattat tccatcctca
     8101 acgataacgg ctccatgttt aacacgccgc cgacatttgc ctggtatcta tctggtctgg
     8161 tctttaaatg gctgaaagcg aacggcggtg tagctgaaat ggataaaatc aatcagcaaa
     8221 aagcagaact gctatatggg gtgattgata acagcgattt ctaccgcaat gacgtggcga
     8281 aagctaaccg ttcgcggatg aacgtgccgt tccagttggc ggacagtgcg cttgacaaat
     8341 tgttccttga agagtctttt gctgcaggcc ttcatgcact gaaaggacac cgtgtggtcg
     8401 gcggaatgcg cgcgtctatt tataatgcca tgccgctgga aggcgttaaa gcgctgacag
     8461 acttcatggt tgagttcgaa cgccgtcacg gttaatgccg aaattttgct taatccccac
     8521 agccagcctg tggggttttt atttctgttg tagagagttg agttcatgga atccctgacg
     8581 ttacaaccca tcgctcgtgt cgatggcact attaatctgc ccggttccaa gagcgtttct
     8641 aaccgcgctt tattgcttgc ggcattagca cacggcaaaa cagtattaac caatctgctg
     8701 gatagcgacg acgtgcgcca tatgctgaat gcattaacag cgttagggtt aagctatacg
     8761 ctttcagccg atcgtacgcg ttgcgaaatt atcggtaacg gcggtccatt acacgcagaa
     8821 ggtgccctgg agttgttcct cggtaacgcc ggaacggcaa tgcgtccgct ggcggcagct
     8881 ctttgtctgg atagcaatga tattgtgctg accggtgagc cgcgtatgaa agagcgcccg
     8941 attggtcatc tggtggatgc gctgcgcctg ggcggggcga agatcactta cctggaacaa
     9001 gaaaattatc cgccgttgcg tttacagggc ggctttactg gcggcaacgt tgacgttgat
     9061 ggctccgttt ccagccaatt cctcaccgca ctgttaatga ctgcgcctct tgcgccggaa
     9121 gatacggtga ttcgtattaa aggcgatctg gtttctaaac cttatatcga catcacactc
     9181 aatctgatga agacgtttgg tgttgaaatt gaaaatcagc actatcaaca atttgtcgta
     9241 aaaggcgggc agtcttatca gtctccgggt acttatttgg tcgaaggcga tgcatcttcg
     9301 gcttcttact tcctggcagc agcagcaatc aaaggcggca ctgtaaaagt gaccggtatt
     9361 ggacgtaaca gtatgcaggg tgatattcgc tttgctgatg tgctggaaaa aatgggcgcg
     9421 accatttgct ggggcgatga ttatatttcc tgcacgcgtg gtgaactgaa cgctattgat
     9481 atggatatga accatattcc tgatgcggcg atgaccattg ccacggcggc gttatttgca
     9541 aaaggcacca ccacgctgcg caatatctat aactggcgtg ttaaagagac cgatcgcctg
     9601 tttgcgatgg caacagaact gcgtaaagtc ggcgcggaag tggaagaggg gcacgattac
     9661 attcgtatca ctcctccgga aaaactgaac tttgccgaga tcgcgacata caatgatcac
     9721 cggatggcga tgtgtttctc gctggtggcg ttgtcagata caccagtgac gattcttgat
     9781 cccaaatgca cggccaaaac atttccggat tatttcgagc agctggcgcg gattagccag
     9841 gcagcctgaa tgaacaacgg gcaataaata gccaaatctt tctttatcaa aacgtcggca
     9901 cattg
//
LOCUS       DAJTSI010000142         9890 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000142 DAJTSI010000000
VERSION     DAJTSI010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9890
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.142"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..303
                     /locus_tag="OO327_003263"
     CDS             <1..303
                     /locus_tag="OO327_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="catalyzes the fromation of 2'O-methylated cytidine
                     or 2'O-methylated uridine at position 32 in tRNA; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR6019692.1"
                     /translation="MAVQVIAYEVRMAWLATQENGEQVEHEETPYPLVDDLERFYGHL
                     EQTLLATGFIRENHPGQVMNKLRRLFTRARPESQELNILRGILASIEQQNKGNKAE"
     gene            573..1061
                     /gene="iscR"
                     /locus_tag="OO327_003264"
     CDS             573..1061
                     /gene="iscR"
                     /locus_tag="OO327_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR6019693.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            1173..2387
                     /gene="iscS"
                     /locus_tag="OO327_003265"
     CDS             1173..2387
                     /gene="iscS"
                     /locus_tag="OO327_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR6019694.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            2415..2801
                     /gene="iscU"
                     /locus_tag="OO327_003266"
     CDS             2415..2801
                     /gene="iscU"
                     /locus_tag="OO327_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR6019695.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            2818..3141
                     /gene="iscA"
                     /locus_tag="OO327_003267"
     CDS             2818..3141
                     /gene="iscA"
                     /locus_tag="OO327_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR6019696.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            3237..3752
                     /gene="hscB"
                     /locus_tag="OO327_003268"
     CDS             3237..3752
                     /gene="hscB"
                     /locus_tag="OO327_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR6019697.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            3769..5619
                     /gene="hscA"
                     /locus_tag="OO327_003269"
     CDS             3769..5619
                     /gene="hscA"
                     /locus_tag="OO327_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR6019698.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            5621..5956
                     /gene="fdx"
                     /locus_tag="OO327_003270"
     CDS             5621..5956
                     /gene="fdx"
                     /locus_tag="OO327_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR6019699.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            5968..6168
                     /gene="iscX"
                     /locus_tag="OO327_003271"
     CDS             5968..6168
                     /gene="iscX"
                     /locus_tag="OO327_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR6019700.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            6227..7510
                     /gene="pepB"
                     /locus_tag="OO327_003272"
     CDS             6227..7510
                     /gene="pepB"
                     /locus_tag="OO327_003272"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR6019701.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            7652..8428
                     /gene="sseB"
                     /locus_tag="OO327_003273"
     CDS             7652..8428
                     /gene="sseB"
                     /locus_tag="OO327_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR6019702.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(9247..9729)
                     /gene="sseA"
                     /locus_tag="OO327_003274"
                     /pseudo
     CDS             complement(9247..9729)
                     /gene="sseA"
                     /locus_tag="OO327_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
BASE COUNT         2432 a         2456 c         2739 g         2263 t
ORIGIN      
        1 atggcggttc aggttattgc ctatgaagtg cgtatggcct ggctggcaac tcaggaaaat
       61 ggcgagcagg tcgagcatga agagacgccg tatccgctgg tcgatgatct ggagcgtttt
      121 tatggtcatc tggaacaaac gctgctggca accggtttta tccgtgaaaa ccatccgggg
      181 caggtgatga ataaattgcg ccgtctgttt acccgtgcgc gcccggaaag ccaggaattg
      241 aatatcctgc gcgggattct ggcttctatt gagcagcaga ataaaggtaa caaggccgaa
      301 taacagtggt tgcctgatgc gacgcttaac gcgtcttatc aggcctacaa tgtacagaat
      361 cgtaggtcgg ataaggtgtt cacaccgcat ccgacaacag gtacaaacgc cacgataaaa
      421 aaatggcact gaaggttaaa tacccgacta aatcagtcaa gtaaatagtt gaccaattta
      481 ctcgggaatg tcagacttga ccctgctatg caataccccc acttttacaa taaaaaaccc
      541 cgggcagggg cgagtttgag gtgaagtaag acatgagact gacatctaaa gggcgctatg
      601 ccgtgaccgc aatgcttgac gttgcgctca actctgaagc gggcccggta ccgttggctg
      661 atatttccga acgtcaggga atttcccttt cttatctgga acaactgttt tcccgtctgc
      721 gtaaaaatgg tctggtttcc agcgtacgtg gaccaggcgg tggttatctg ttaggcaaag
      781 atgccagcag catcgccgtt ggcgaagtga ttagcgccgt tgacgaatct gtagatgcca
      841 cccgttgtca gggtaaaggc ggctgccagg gcggcgataa atgcctgacc cacgcgctgt
      901 ggcgtgattt gagcgaccgt ctcaccggtt ttctcaacaa cattacttta ggcgaactgg
      961 ttaataacca ggaagtgctg gatgtgtctg gtcgtcagca tactcacgac gcgccacgca
     1021 cccgcacaca agacgcgatc gacgttaagt tacgcgctta ataaaaagaa ttcagaatca
     1081 ggccggagtg ctaaatactc cgtaaacacg gtcgtacatc cagccggtag cctgattcct
     1141 tgcattgagt gatgtacgga gtttatagag caatgaaatt accgatttat ctcgactact
     1201 ccgcaaccac gccggtggac ccgcgtgttg ccgagaaaat gatgcagttc atgacgatgg
     1261 acggaacctt tggtaacccg gcctctcgtt ctcaccgttt cggctggcag gctgaagaag
     1321 cggtagatat cgcccgtaat cagattgccg atctggtcgg cgctgatccg cgtgaaatcg
     1381 tctttacctc tggtgcaacc gaatctgaca acctggcgat caaaggtgca gccaactttt
     1441 atcagaaaaa aggcaagcac atcatcacca gcaaaaccga acacaaagcg gtactggata
     1501 cttgccgtca gctggagcgc gaaggtttcg aagtcaccta cctggcaccg cagcgtaacg
     1561 gtattatcga cctgaaagaa ctcgaagcgg cgatgcgtga cgacaccatc cttgtttcca
     1621 tcatgcacgt aaataacgaa atcggcgtgg tgcaggatat cgcggctatc ggcgaaatgt
     1681 gccgtgctcg tggcattatc tatcacgttg atgcaaccca gagcgtgggc aaactgccaa
     1741 tcgacctgag ccagttgaaa gttgacctga tgtctttctc cggtcacaaa atctatggcc
     1801 cgaaaggtat cggtgcgctg tatgtgcgtc gtaaaccgcg tgtacgcatc gaagcgcaaa
     1861 tgcacggcgg cggtcacgag cgcggtatgc gttccggcac tctgcctgtt caccagatcg
     1921 tcggaatggg cgaagcctat cgcatcgcaa aagaagagat ggcgaccgag atggaacgtc
     1981 tgcgcggcct gcgtaaccgt ctgtggaacg gcatcaaaga tatcgaagaa gtttacctga
     2041 acggtgacct ggaacacggt gcgccgaaca ttctcaacgt cagcttcaac tacgttgaag
     2101 gtgagtcgct gattatggcg ctgaaagacc tcgcagtttc ttcaggttcc gcctgtacgt
     2161 cagcaagcct cgaaccgtcc tacgtgctgc gcgcgctggg gctgaacgac gagctggcac
     2221 atagctctat ccgtttctct ttaggtcgtt ttactactga agaagagatc gactacacca
     2281 tcgagttagt tcgtaaatcc atcggtcgtc tgcgtgacct ttctccgctg tgggaaatgt
     2341 acaagcaggg cgtggatctg aacagcatcg aatgggctca tcattaatcg gtatcggaat
     2401 caggagaatt tataatggct tacagcgaaa aagttatcga ccattacgag aatccgcgta
     2461 acgtgggttc ctttgataac aacgacgaga acgtcggcag cggcatggtg ggtgcaccgg
     2521 cctgtggcga cgtaatgaag ttgcagatta aagtcaacga tgaaggtatc attgaagacg
     2581 cgcgttttaa aacttacggc tgcggttccg ctatcgcttc cagctccctg gtcaccgaat
     2641 gggtgaaagg gaagtctctc gacgaagcgc aggcgatcaa aaacaccgat attgctgaag
     2701 aacttgaact gccgccggtg aaaattcact gttctattct ggcagaagac gcgatcaaag
     2761 ccgccattgc ggactataaa agcaaacgtg aagcaaaata agagttgagg tttggttatg
     2821 tcgattacac tgagcgacag tgcagcagcg cgagtaaata cctttctggc taaccgcggc
     2881 aaagggtttg gcctgcgtct gggcgtgaga acctccggat gttcaggtat ggcttatgta
     2941 ctggaatttg ttgacgaacc gacgccggaa gacatcgtgt ttgaagacaa aggcgtgaaa
     3001 gtcgtggtcg atggcaaaag cctgcaattt ctggacggta cgcagctgga cttcgtaaaa
     3061 gaaggcctga atgaagggtt taaattcacc aacccgaacg ttaaagatga gtgtggttgc
     3121 ggcgaaagct tccacgtttg atgcgcatac cgataacccc accgtggtcg cctgcgcgtg
     3181 gggtttgttt tacctgattc gccgttatcg cggcggatcg cagccctgag aatgttatgg
     3241 attacttcac cctctttggc ttgcctgccc gttatcaact cgatacccag gcactgagcc
     3301 tgcgttttca ggatctacaa cgtcagtatc atcctgataa attcgccagc ggaagcctgg
     3361 cggaacaact cgccgccgta cagcaatctg caaccattaa ccaggcctgg caaacgctgc
     3421 gtcatccgtt aatgcgcgcg gaatatttgc tttctttaca cggttttgat ctcgccagcg
     3481 agcagcatac tgtgcgcgac accgcgttcc tgatggaaca gttggagctg cgcgaagagc
     3541 tggacgagat cgaacaggcg aaagatgaag cgcggctgga aagctttatc aaacgtgtga
     3601 aaaagatgtt tgatacccgc catcagttga tggttgaaca gttagacaac gaggcgtggg
     3661 acgcggcggc ggatacggtg cgtaagctgc gttttctcga taaactgcgg agcagtgccg
     3721 aacaactcga agaaaaactg ctcgattttt aatttctgga agctaaacat ggccttatta
     3781 caaattagtg aacctggttt gagtgctgcg ccgcatcagc gtcgtctggc ggctggtatt
     3841 gacctgggca caaccaactc gctggtggcg acagtgcgca gcggtcaggc cgaaacgtta
     3901 gccgaccatg aaggccgtca cctgctgcca tctgttgttc actatcaaca gcaagggcat
     3961 tcggtgggtt atgacgcgcg tactaacgca gcgcttgaca ccgccaacac cattagttct
     4021 gttaaacgcc tgatggggcg ctcgctggct gatatccagc aacgctatcc gcatctgcct
     4081 tatcaattcc aggccagcga aaacggcctg ccgatgattg aaacggcggc ggggctactg
     4141 aacccggtgc gcgtttctgc ggacatcctc aaagcactgg cggcgcgggc aactgaagcc
     4201 ctggcaggcg agctggatgg tgtagttatc accgttccgg cgtactttga cgatgcccag
     4261 cgtcagggca ccaaagacgc ggcgcgtctg gcggggttgc atgttctgcg cttacttaac
     4321 gaaccgactg ctgcggctat cgcctacggg ctggattccg gtcaggaagg cgtgatcgct
     4381 gtttatgacc tcggtggcgg gacgtttgat atttccattc tgcgcttaag tcgcggcgtg
     4441 tttgaagtgc tggcaaccgg cggtgattcc gcgctcggcg gcgatgattt cgaccatctg
     4501 ctggcggatt acattcgcga gcaggcggac attcctgatc gtagcgataa ccgcgttcag
     4561 cgtgaactgc tggatgccac cattgcagcc aaaatcgcgc tgagcgatgc ggactccgtg
     4621 accgttaacg ttgcgggctg gcagggcgaa atcagccgtg aacaattcaa tgaactgatc
     4681 gcgccactgg taaaacgaac cttactggct tgtcgtcgcg cgctgaaaga cgcgggtgta
     4741 gaagctgacg aagtgctgga agtggtgatg gtgggcggtt ctactcgcgt gccgctggtg
     4801 cgtgaacggg taggcgaatt tttcggtcgt ccaccgctga cttccatcga cccggataaa
     4861 gtcgtcgcta ttggcgcggc gattcaggcg gatattctgg tgggtaacaa gccagacagc
     4921 gaaatgctgc tgcttgatgt gatcccactg tcgctgggcc tcgaaacgat gggcggcctg
     4981 gtggagaaag tgattccgcg taataccact attccggtgg cccgcgctca ggatttcacc
     5041 acctttaaag atggtcagac ggcgatgtct atccatgtaa tgcagggtga gcgcgaactg
     5101 gtgcaggact gccgctcact ggcgcgtttt gcgctgcgtg gtattccggc gctaccggct
     5161 ggcggtgcgc atattcgcgt gacgttccag gtcgatgccg acggtctttt gagcgtgacg
     5221 gcgatggaga aatccaccgg cgttgaggcg tctattcagg tcaaaccgtc ttacggtctg
     5281 accgatagcg aaatcgcttc gatgatcaaa gactcaatga gctatgccga gcaggacgta
     5341 aaagcccgta tgctggcaga acaaaaagta gaagcggcgc gtgtgctgga aagtctgcac
     5401 ggcgcgctgg ctgctgatgc cgcgctgtta agcgccgcag agcgtcaggt cattgacaat
     5461 gctgccgctc acctgagtga agtggcgcag ggcgatgatg ttgacgccat cgaacaagcg
     5521 attaaaaacg tagacaaaca aacccaggat ttcgccgctc gccgcatgga ccagtcggtt
     5581 cgtcgtgcgc tgaaaggcca ttccgtggac gaggtttaat atgccaaaga ttgttatttt
     5641 gcctcatcag gacctctgcc ctgatggcgc tgttctggaa gctaatagcg gtgaaaccat
     5701 tctcgacgca gcgctgcgta acggtattga gattgaacac gcctgtgaaa aatcctgtgc
     5761 ttgcaccacc tgccactgca tcgttcgtga aggttttgac tcactgccgg aaagctcaga
     5821 gcaggaagac gacatgctgg acaaagcctg gggactggag ccggaaagcc gtttaagctg
     5881 ccaggcgcgc gtcaccgacg aagatttagt ggtcgaaatc ccgcgttaca ctatcaacca
     5941 tgcgcgtgag cattaacaga ggttagtatg ggacttaagt ggaccgatag ccgcgaaatt
     6001 ggcgaagcac tgtacgatgc gtatcccgat cttgatccga aaacggttcg attcaccgat
     6061 atgcatcagt ggatttgcga tctggaagat ttcgacgacg acccgcaggc atccaacgag
     6121 aaaatcctcg aagcgatttt gttagtctgg ctggacgaag ccgagtgaat gaacgggctg
     6181 cctgggggta gcccgttttg ctgttaaacg aataaggata actaaaatga cagaagcgat
     6241 gaagattacc ctctctaccc aacctgccga cgcgcgctgg ggagaaaaag caacttacag
     6301 cattaataat gacggcatta ccctgcattt gaacggggca gacgatctgg ggctgatcca
     6361 gcgtgcggcc cgcaagattg acggtctggg catcaagcat gttcagttaa gcggtgaagg
     6421 ctgggatgcg gatcgctgct gggcattctg gcaaggttat aaaggcccga aaggcacgcg
     6481 taaagtggag tggccggatc tggacgatgc ccagcgccag gaactggata accgcctgat
     6541 gatcatcgac tgggtgcgtg acaccatcaa cgcaccggca gaagaattgg gaccatcgca
     6601 actggcacag cgtgctgttg atctgatcag caacgtcgcg ggcgatcgtg tgacttatcg
     6661 gatcaccaaa ggcgaagatc tgcgtgagca aggttatatg gggctgcaca ctgtcgggcg
     6721 cggttcagaa cgttctccgg tattgctggc gctggattac aacccaactg gcgataaaga
     6781 agcaccggtg tacgcgtgcc tggtaggtaa aggtatcact tttgactccg gcggctacag
     6841 catcaaacag actgcgttta tggactcgat gaagtcggac atgggcggcg cggcaacggt
     6901 taccggggcg ctggcatttg ccattatgcg cggactgaac aagcgcgtga agctgttcct
     6961 ctgctgtgcg gataacctga ttagcggcaa tgcgttcaag ctgggcgata tcatcaccta
     7021 tcgcaacggt aaaaaagttg aagtgatgaa cactgatgcc gaagggcgtc tggtgcttgc
     7081 cgatggtctg attgatgcca gtgcgcagaa accggaaatg atcattgatg cggcgaccct
     7141 caccggggcg gcgaaaactg cgctgggtaa tgattatcac gcgctgttca gttttgacga
     7201 tgcgcttgcc ggtcgtttac tggcgagtgc ctcacaagag aacgaaccat tctggcgtct
     7261 gccgctggcg gaattccacc gcagccagct gccgtctaac tttgccgaac tgaacaatac
     7321 cggaagcgcg gcgtatccgg caggcgcgag cacggcggcg ggcttcctgt cgcactttgt
     7381 tgagaactat cagcaaggct ggctgcatat cgactgctcg gcgacttacc gtaaagcgcc
     7441 ggttgaacag tggtctgcgg gcgctacggg acttggtgtg cgcacgatag ctaatctgtt
     7501 aacggcgtaa actaatatgc cggatgcggc gtaaacgcct tatccggcct acaaattctg
     7561 caaattcaac aaattgcgaa tcccttgtag gcctgataag cgtagcgcat caggcatttg
     7621 cgccccgaac gttgaatgaa ttgtggttct tatgtccgaa acaaaaaacg aacttgaaga
     7681 cctgctggaa aaagcagcaa ccgaaccggc gcaccgcccg gcctttttcc gtactctact
     7741 ggaatccacc gtctgggtgc ctggtacggc ggcgcagggc gaggctgtgg ttgaagatag
     7801 tgcgcttgat ttacagcact gggaaaaaga agacggcacc agcgtcattc cttttttcac
     7861 ctcgttagaa gcgcttcaac aggcggttga agacgaacag gcatttgtcg taatgcccgt
     7921 tcgcacattg tttgagatga cccttggcga aacgctcttc cttaatgcca aactgccgac
     7981 cggtaaagaa tttatgccgc gtgaaatcag tttgttgatt ggtgaagagg gaaatccgct
     8041 gagcagccag gaaatcctgg aaggcggtga atcgctgata ttatcggaag tcgcagagcc
     8101 gccagcacaa atgattgatt cactcaccac cttatttaaa accattaagc cggtgaagcg
     8161 tgcttttatt tgttcaatta aagagaacga agaggcacag cctaatttac ttattggcat
     8221 tgaagccgat ggtgatatcg aagaaattat tcaggcgacg ggaagtgtag cgaccgatac
     8281 attacctggc gatgaaccaa tcgatatttg tcaggtgaaa aaaggggaaa aaggaattag
     8341 ccactttatt accgaacata ttgcgccatt ctatgaacgt cgctggggtg gttttttgcg
     8401 tgactttaaa caaaaccgga taatctaaaa tatgccgccc taaagggcgg cattcgggct
     8461 tacttgctgc tctgaaggtt gttaaccacc agtaaattgt cgccggagag actccggtta
     8521 ttatttactt gggaaaacag cgcaccagaa acaataagta cgatacagaa gcaccgtatc
     8581 ttcattgtgc ggtccatgcg aagaccgcat cacgacagca ccactggcga tattgccgcg
     8641 atagcaagcg ctgagacgcg cttgctctaa cggtcgcgga acatcctttc cttaagccag
     8701 cgcccgcttg cgagacggcg gaaagggccg cagtggataa tacactaaca gaacaataat
     8761 aactctcttt tgcttgacaa aaagagagtt actggcgagt attgtctccg ctgagacgcg
     8821 cttgcattac tacggtaata caacctgctg ggaaggccgc gaaccggacc agcaacaaat
     8881 aaccgcctga ttttggcggt tttttgtttt ttaaaggaaa gtaaatttac atgtttaagt
     8941 aatatctttt accggttaga atttttaatt ttgagcagga tttgtttata tttgtctgaa
     9001 ttcggactaa taagccaggt tattttatta ccgaacacgg cggaaagggc cggtgtcgat
     9061 aatacgctaa ataaacaata attactctct tttgcttgac aaaaagagag ttactggtga
     9121 gtattgtttt gctgtgaagc acctgcgttg ctcatgtaac acaagtcaac ctgctggaaa
     9181 ggccgcgaac cagaccagca ataaaaaaac gccaaattcg gcggtttttt attgcctgta
     9241 atatacttat ttcactggct caaccggtaa atctgcccgc gcgccccatt cactccatgc
     9301 gccgtcgtac agtttcacgt ttggcacatc cagcgtcgcg agtgctaaca aaaccacggc
     9361 tgccgttaca ccagagccgc agctgacgat aatcggtttg tcatagctga cgccgcgacc
     9421 aaaaaatatc gcatccagtt catcggtcgt ttttagttcg ccttcgcgca ccagttccgt
     9481 ccacggaacg ttcaatgcac cgggaatatg tccgcgacgt aaacctgggc gaggttcatc
     9541 aacttctgcg ttaaaacgtg cagccgggcg ggcatcaata atttgcgccg tattttcatg
     9601 gcttgccaat aacacatcgg ttactttcac cactgcttca ggattaaacg cggcgttaaa
     9661 ctctccttcc ggcagctcta ctgcaccttc ttctaatagc aggtcatcgc gctgccagcc
     9721 tgcaagtccc cgttacttta ttcgtacccc ttataatggg gtgttagcca gccagacccg
     9781 gcatgattac tgcccccagt cgtccatgat ccgggggggg atgtcaccgg gtctggtggg
     9841 gcgctggtaa ccgctaatag gggtcaggtc aggcactttt gccgggaccg
//
LOCUS       DAJTSI010000143         9773 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000143 DAJTSI010000000
VERSION     DAJTSI010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9773
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.143"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            392..2023
                     /gene="oppA"
                     /locus_tag="OO327_003275"
     CDS             392..2023
                     /gene="oppA"
                     /locus_tag="OO327_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR6019703.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2109..3029
                     /gene="oppB"
                     /locus_tag="OO327_003276"
     CDS             2109..3029
                     /gene="oppB"
                     /locus_tag="OO327_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR6019704.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3044..3952
                     /gene="oppC"
                     /locus_tag="OO327_003277"
     CDS             3044..3952
                     /gene="oppC"
                     /locus_tag="OO327_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR6019705.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3964..4977
                     /gene="oppD"
                     /locus_tag="OO327_003278"
     CDS             3964..4977
                     /gene="oppD"
                     /locus_tag="OO327_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR6019706.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4974..5978
                     /gene="oppF"
                     /locus_tag="OO327_003279"
     CDS             4974..5978
                     /gene="oppF"
                     /locus_tag="OO327_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR6019707.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6031..6360)
                     /locus_tag="OO327_003280"
     CDS             complement(6031..6360)
                     /locus_tag="OO327_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR6019708.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEISDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6395..7855)
                     /gene="cls"
                     /locus_tag="OO327_003281"
     CDS             complement(6395..7855)
                     /gene="cls"
                     /locus_tag="OO327_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR6019709.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7998..8171
                     /locus_tag="OO327_003282"
     CDS             7998..8171
                     /locus_tag="OO327_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR6019710.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8226..9479)
                     /gene="kch"
                     /locus_tag="OO327_003283"
     CDS             complement(8226..9479)
                     /gene="kch"
                     /locus_tag="OO327_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR6019711.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2563 a         2286 c         2433 g         2491 t
ORIGIN      
        1 tgaaagatgt ttgttaaggc atggatgcaa gctatagatt ctgatacggt caataaaaga
       61 gaatcgctta acaattttgc aaaatgtatt ggcgagtaag aaccgcattt ggtactttcc
      121 gggcaaccgc cagacgattc tttattggta atgagaataa ttaacaatta aagagcgtcg
      181 cgaaagaata atgtgtctcg acaggggaga cacagtacga atcgacataa ggtgatcgtc
      241 tgaatcacca gaataaataa agtcggtgat agtaatacgt aacgataaag taacctgaca
      301 gcagaaagtc tccgagcttg tgcagggtcc caatccggga ttacacatgc tggttaatac
      361 cagtaattat aatgagggag tccaaaaaac aatgaccaac atcaccaaga gaagtttagt
      421 agcagctggc gttctggctg cgctaatggc agggaatgtc gcgctggcag ctgatgtacc
      481 cgcaggcgtc acactggcgg aaaaacaaac actggtacgt aacaatggtt cagaagttca
      541 gtcattagat ccgcacaaaa ttgaaggtgt tccggagtct aatatcagcc gagacctgtt
      601 tgaaggctta ctggtcagcg atcttgacgg tcatccagca cctggcgtcg ctgaatcctg
      661 ggataataaa gacgcgaaag tctggacctt ccatttgcgt aaagatgcga aatggtctga
      721 tggcacgcca gtcacagcac aagactttgt gtatagctgg caacgttctg ttgatccgaa
      781 cactgcttct ccgtatgcca gttatctgca atatgggcat atcgccggta ttgatgaaat
      841 tcttgaaggg aaaaaaccga ttaccgatct cggcgtgaaa gctattgatg atcacacatt
      901 agaagtcacc ttaagtgaac ccgttccgta cttctataaa ttacttgttc acccatcaac
      961 ttcaccggtg ccaaaagccg ctatcgagaa attcggcgaa aaatggaccc agcctggtaa
     1021 tatcgtcacc aacggtgcct ataccttaaa agattgggtc gtaaacgaac gaatcgttct
     1081 tgaacgcagc ccgacctact ggaacaacgc gaaaaccgtt attaaccagg taacctattt
     1141 gcctattgct tctgaagtta ccgatgtcaa ccgctaccgt agtggtgaaa tcgacatgac
     1201 gtataacaac atgccgatcg aattgttcca gaagctgaaa aaagagatcc cggacgaagt
     1261 tcacgttgat ccatacctgt gcacttacta ttacgaaatt aacaaccaga aaccgccatt
     1321 caacgatgtg cgtgtgcgta ccgcactgaa actaggtatg gaccgcgata tcattgttaa
     1381 taaagtgaaa gcgcagggcg acatgcccgc ctatggttat actccaccgt atactgatgg
     1441 cgcaaaattg actcagccag aatggtttgg ctggagccag gaaaaacgta acgaagaagc
     1501 gaaaaaactg ctggctgaag cgggttatac cgcagacaaa ccgttgacca tcaacctgcg
     1561 gtataacacc tccgatctgc ataaaaagct ggcgattgct gcctcttcat tgtggaagaa
     1621 aaacattggt gtaaacgtca aactggttaa ccaggagtgg aaaacgttcc tcgacacccg
     1681 tcaccagggc acttttgatg tggcccgtgc aggctggtgt gcagactaca acgaaccaac
     1741 ttccttcctg aacaccatgc tgtcgaacag ctcgatgaat accgcgcatt ataagagccc
     1801 ggcctttgac agcattatgg cggaaacgct gaaagtgact gacgaggcgc agcgcacagc
     1861 tctgtacact aaagcagaac aacagctgga taaggattcg gccattgttc ctgtttatta
     1921 ctacgtgaat gcgcgtctgg tgaaaccgtg ggttggtggc tataccggca aagatccgct
     1981 ggataatacc tatacccgga atatgtacat tgtgaagcac taatggcaat acgtggggca
     2041 ggagtgtcct gctccacggt gtctgatttt tatcgcatta cagaaggcac aggccagaag
     2101 gtagggcaat gttaaaattt attctacgtc gttgtctgga agcgattccg acgctattta
     2161 ttcttattac tatttcgttc tttatgatgc gcctcgcacc gggaagccct tttaccggcg
     2221 aacgtacttt accgccagaa gtgatggcca atatcgaagc gaaatatcat cttaatgatc
     2281 cgatcatgac acagtatttc agctacctga aacaactggc gcatggcgat ttcggtccat
     2341 cgtttaaata taaagattat tcggtcaatg acctggtggc atccagtttt cccgtttctg
     2401 ccaaactggg agccgccgca tttttccttg cggtaatact gggtgttagt gctggcgtta
     2461 ttgccgcatt aaaacaaaac accaaatggg actataccgt gatggggctg gcaatgaccg
     2521 gggttgttat ccccagtttt gtggttgcgc cattattagt catgatattt gcgatcattt
     2581 tgcattggct gccgggcggt ggctggaatg gtggggcgct taaattcatg atattgccga
     2641 tggtggcgtt gtcactcgcc tatatcgcca gtattgcgcg tattacccgt ggctctatga
     2701 ttgaagtatt acactccaac tttattcgta ctgcccgggc gaaagggtta cctatgcggc
     2761 ggatcatttt acgccatgca ttaaaacctg ctctgttacc cgtgctctcc tatatgggcc
     2821 ccgcatttgt cggcattatt accggttcta tggtcatcga aaccatttat ggtttgccgg
     2881 ggattgggca actgttcgtt aatggcgcat tgaaccgtga ctattcctta gtgttaagcc
     2941 tgaccatcct ggttggtgcg ttaaccattt tgtttaatgc cattgtcgat gtgctatatg
     3001 cggttatcga cccgaaaatc cgttactgat actggagctc gcgatgatgt taagtaagaa
     3061 aaacagcgag acgctggaaa atttcagtga aaagctggag gtcgaagggc gcagcttgtg
     3121 gcaggacgca cgtcgacgtt ttatgcataa ccgtgcggcg gttgccagtc tgatagtgct
     3181 ggtgctgatc gcgttatttg taatcctggc accgatgctt tcccagtttg cctatgacga
     3241 tactgactgg gcgatgatgt ccagcgcccc ggatatggag tccggtcact actttggtac
     3301 tgactcatcc ggtcgcgacc tgcttgtgcg cgttgcgatt ggcgggcgta tctcactcat
     3361 ggtcggtgtt gctgcggcac tggtggcagt ggtcgtgggg acactttatg gttcgctttc
     3421 cggttatctg ggcggtaaag tggattcggt aatgatgcgt ctgctggaaa tcctcaactc
     3481 cttcccattc atgttcttcg tcattttgct ggtgaccttt ttcggccaaa acatcctgct
     3541 gattttcgtg gcgattggca tggtttcctg gctggatatg gctcgtattg tgcgtgggca
     3601 aaccctgagt ctgaagcgca aagagtttat tgaggcggca caagttggcg gtgtatcgac
     3661 gccgggcatt gttattcgcc acattgtgcc gaacgtactc ggtgtggtgg tggtctacgc
     3721 atcgctactg gtgcccagca tgatcctctt tgaatctttc cttagcttcc tggggttggg
     3781 tacgcaagag ccgttaagca gctggggggc attgctgagt gatggcgcga actcgatgga
     3841 agtctctcca tggttactgt tgttcccagc gggattcctt gtggtgacac tgttttgttt
     3901 caactttatc ggcgatggct tgcgtgatgc cctcgacccg aaagatcgtt aaggagtgca
     3961 gccatgagcg taattgaaac tgcaactgtg ccgctcgcac aacaacaggc tgacgcactg
     4021 ctgaacgtga aagatttgcg tgtcaccttt agtaccccgg acggcgacgt cacggcggtc
     4081 aatgatttaa atttttccct acgtgccgga gaaacgctgg gcattgtagg tgagtctggt
     4141 tcgggtaaat cgcaaactgc atttgcgttg atgggcctgt tggctgccaa cggacgcatt
     4201 ggcggatcgg caaccttcaa tgggcgtgaa atcctcaatt tgccagagcg tgaactcaat
     4261 aaactgcgcg ctgaacaaat ctcaatgatt tttcaggacc caatgacttc gttgaatccc
     4321 tatatgcgcg tcggtgagca gttgatggaa gtgctgatgc tgcataagaa catgagcaaa
     4381 gctgaggcgt ttgaagagtc ggtgcggatg ctcgatgcgg taaaaatgcc ggaagcacgt
     4441 aaacgcatga aaatgtaccc gcacgaattt tctggcggca tgcgtcagcg agtcatgatt
     4501 gcgatggcat tgctatgtcg acctaagttg ctgattgcgg atgaacccac tacagcgctg
     4561 gacgtcaccg tacaggcgca gatcatgacg ctattgaatg aactgaagcg ggaatttaat
     4621 actgccatca ttatgatcac ccacgatctt ggcgtggtgg cggggatctg tgacaaagtg
     4681 ctggtaatgt acgccgggcg cacgatggaa tatggcaatg cgcgcgatgt cttttatcaa
     4741 cccgttcatc cttattctat cggtttgctc aacgcggtgc cgcgtcttga tgcggaaggt
     4801 gaaacaatgt tgaccatccc gggtaatccg ccaaacttgc tgcgattacc gaaaggttgc
     4861 ccgttccagc cacgttgtcc acatgcgatg gaaatttgta gtagcgcacc gccgctggaa
     4921 gagtttacgc ctggccgtct gcgtgcttgc tttaaaccgg tggaggaact gttatgaatg
     4981 ctgtaactga aggaagaaaa gtcctccttg aaatcgccga tcttaaagtg cactttgaaa
     5041 tcaaagatgg caaacagtgg ttctggcaac cgccgaaaac gctcaaagcc gtcgatggtg
     5101 tcactcttcg cctgtatgaa ggggaaacat taggtgtggt aggggaatcg ggatgcggta
     5161 agtccacctt tgctcgcgcc atcatcggtt tggtcaaggc gaccgacggt catgttgcct
     5221 ggttaggtaa agagttgctg ggcatgaagc ccgatgaatg gcgtgccgtt cgcagtgata
     5281 ttcagatgat tttccaggat ccgttggcat cgctaaaccc gcgtatgacc atcggcgaga
     5341 tcatcgctga accactgcgt acttatcatc cgaaaatgtc acgccaggaa gttcgcgagc
     5401 gcgtgaaggc gatgatgctg aaagtcgggt tattgcctaa cctgattaac cgctatccgc
     5461 atgagttctc cggtgggcag tgccagcgta tcgggattgc tcgtgctctt attcttgaac
     5521 cgaagctgat tatctgcgat gagccggttt cggcgctgga cgtgtcaatt caggcgcagg
     5581 tggttaacct gctccagcag ctgcaacgtg agatgggatt gtcattaatt tttattgctc
     5641 atgacctggc cgtggtaaaa cacatttccg atcgtgtgtt ggtgatgtat ctcggccatg
     5701 cagtagaact ggggacctat gatgaggtct accacaatcc actacatcct tacaccaggg
     5761 cattgatgtc ggcagtcccc atacctgatc cggatctgga gaagaacaaa accatccagt
     5821 tactggaagg ggaattaccg tcgccgatca acccgccttc cggttgtgtt ttccgtaccc
     5881 gttgcccgat tgccggtccg gagtgcgcca aaacacgtcc tgttctggag gggagtttca
     5941 gacacgccgt ttcttgcctg aaagtcgatc cgctttaaaa acaataaggg ctgacagttg
     6001 tcagcccttt ttcacgctaa aagcgattat ttattcccgc cagatgatat ggcaaagttt
     6061 gtgatctttt tcgcggcata acagaatgcg ggcgaaaaca tcgctgattt caccatcttc
     6121 actgtccgcc agaccaatca ccacttcggc aaaaaagtcc gggttcaaat cgaaatcaac
     6181 atgttcctgc cagtcttccg ccggatcgaa taactccgcg ccgccgcgct cttcaaactg
     6241 aagattgaac agcagaacat cagctggatc gagattgtcg gcagccagtt cgagaaaaat
     6301 atcgtaggcc tgctcaagcg tttcatcttc agtcaggcga ttgtttagat ccatatccat
     6361 agtcactacc tgtttaacct ctgttggcga cgttttacag caacgggctg aagaagtaaa
     6421 acagtcgctc ggcgacacgt tgccataatg gacgttttag ccataaacgg gcatcgagca
     6481 gacgtgaacg cgaaatataa tcgtcctgaa cggcggcaag gtctgcacca aaacctttat
     6541 cgtcgattgc caaggtaatc tcgaaattca gccacagact acgcatatca aggttaacgg
     6601 tgccaaccag acttagttcg ccatcgacca gcacgctctt ggtatgcagt aacccgcctt
     6661 caaactgata aattttaacc ccagcagcaa gcagttccgt aaagaatgcg cgactggccc
     6721 agccgaccag catcgagtca ttttttcgcg gaaggataat actgacatcc accccgcgct
     6781 gcgccgccgt gcaaatcgca tgaagtaaat catcgcttgg cacaaagtag ggcgtggtca
     6841 tgatcaaata ttcatgcgcc gaataagccg cagtcaataa tgcctggtga atgagatctt
     6901 ccggaaagcc ggggccagaa gcaattgtgt gaatggtgtg accgctggcc tgttcaaacg
     6961 gcataatatt gacatctggt ggtggcggca gaatacgttt tccggtttca atctcccagt
     7021 cgcaggaata aataatcccc atcgcggtgg cgatggggcc ttccatacgc gccatcagat
     7081 caatccattg ccctacgccc gcatcttgtt tgaagtagcg aggatcgacc atattcatgc
     7141 tgccggtgta cgcgatgtaa ttatcgatca tgatcatctt gcgatgttgg cgcaggtcca
     7201 tacggcgtaa aaacacacgc atcagattga cctttaaggc ttcgaccact tcaataccgg
     7261 cattacgcat tagctcgggc cacgggctgc ggaaaaaagc cacactcccg gcggagtcga
     7321 gcatcaatcg gcaatgaata ccacgccgtg cagccgccat taatgattca gccacctggt
     7381 ccgccatgcc gccgggctgc cagatataaa acaccatctc aatattatgg cgcgcgagct
     7441 ggatgtcgcg gattaacgcc tgcatcacat catctgactc ggtcatcagc tgtagctgat
     7501 tccctttgac cccagcgatc ccctgacgac gctcgcaaag cttgaataat ggcgcagcga
     7561 cactgctatt ttcttcggcg aagatatgct tacaggcttt aaggtcgtta agccattttg
     7621 cggtggaagg ccacatcgct ctggcgcgct cagcgcggcg tttgcctaaa tggagctcgc
     7681 caacggcaag ataggcaata attccgacta acggcagaat gtaaataatc aacagccagg
     7741 ccatcgcgga gggaactgcg cgtcgtttca ttagaatgcg taaagttacg cctgcaatga
     7801 gcaaccagta tcccagaatg gccaaccaac tcaccaacgt ataaacggtt gtcatagatt
     7861 agaaatcctt ttgaaagcgc attgttatga gtttacgcat atggattcat ctggcaaata
     7921 aaaacgcggg aaaagcactg gtcagcgtcg ggggcggggg tataatgacc attctgttat
     7981 tgcatagagt agttaacatg aagcggagta gaacggaagt ggggcgctgg cgcatgcagc
     8041 gtcaggctag ccgacgtaaa tcgcgttggc ttgaggggca atcgcgccga aatatgcgta
     8101 tccacagcat caggaagtgc attctaaaca aacagcgtaa ctcgttattg tttgcgatct
     8161 acaatatcta aatgtactgg gcaccattgc ggtgcccaat aagtttactc ttccgtcttt
     8221 aaactctatt tttgcgccga ttctttactg tcagctttcg tttcttgttc atcgttatcg
     8281 ctgaaaatac catgacctga gttcaacaac attgaaacga gcatatcatt attaatctct
     8341 tcaccattta aaactcgcgc cagaatttcg ctgccaaaca gttgcggtga gagaatgata
     8401 tccggatgta ccatcttaat cttattcagg tttttgctat cactgacggc gagaactgtt
     8461 ttgacatcac tgctcatatc ttttgccgag agtacaacaa acgcgttatc tgcatcgtta
     8521 tcactcagcg ccagaatggc ccggcaacga tcgattcccg ctttctttaa tactgaactg
     8581 tcattactgt caccggggat aacatcagcg ttatcgccta agcgttgctc aagttgcttg
     8641 atatcatctt caggcaagtt gctgataacc gttacgtttt gtccgcgttg attcagttgc
     8701 aggatcgtat tgatggcgag aatcgaatgt ccgcaaacaa taaaatgatc tttacgatgc
     8761 attgtatgat tgtttccttt taaaagtttg ttgaatcccc cgcggataag cggaccaaaa
     8821 attgaggtca tagatgtggc aaaaacggta atgccggaaa taatgaccga aatagtgaac
     8881 aatcgtgctg attcagaaac agggacaata tctccatagc cgacggttga catggtttct
     8941 atcgaaaaat agaacgcggt catcaaactt tctattcgcg gattaaaacc ttcgcttaaa
     9001 taaagcgcac cgtaggttga gtaaaacagt aatgtcgtga aactaataaa tgcaaaaatt
     9061 gtcccggctg cggcgctact gtgggagaag tctttgcgca gtatcagcaa aaacaccagc
     9121 gtaaaaatgc aaaatccaat actaaatttc agccagggat aaaaatgcag ggtgtaaatt
     9181 agcgctatca acaacaaaat gatactgatt gcccatgcga gctttgcccg gaacagtaag
     9241 ccaatggagt tcaggacaag aaagacgccg agcataaata gtggggcgtt tgccagcaag
     9301 gagagatcca actctgaaaa tgctttgata tggaaaatat caagcaaatt gaccgacata
     9361 ccatagattg ttttaaaaat aagcaatcca tttaaaaaga cggccagcgc gagaatgtcg
     9421 tgccgtaggg tcacccataa atttgttgct gtttgtttga atgtagccca gtgactcact
     9481 gatacctctg taaccacaat tttgtttaat tatcttagcg tgcacatttt ctgaacatac
     9541 atgcagcgcg tgaatgtgtt aaacaatagt agatgacttt atccgcagta atcaaaggtt
     9601 ttgagtgagc aactctgcgt caggaaaggg cttttttcct gaacatgctc acatcatata
     9661 aagaaataca gataaagtta ttatctgctt gtggtagtga atgcactgac cggctataag
     9721 gaaaggccaa acaagaacac ggttgcaaaa accgtgccct taaatattgg atc
//
LOCUS       DAJTSI010000144         9680 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000144 DAJTSI010000000
VERSION     DAJTSI010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9680
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.144"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(26..214)
                     /locus_tag="OO327_003284"
     CDS             complement(26..214)
                     /locus_tag="OO327_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6019712.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMVMTPTY"
     gene            complement(433..1410)
                     /gene="epmA"
                     /locus_tag="OO327_003285"
     CDS             complement(433..1410)
                     /gene="epmA"
                     /locus_tag="OO327_003285"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR6019713.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1735..3543
                     /gene="frdA"
                     /locus_tag="OO327_003286"
     CDS             1735..3543
                     /gene="frdA"
                     /locus_tag="OO327_003286"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR6019714.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3536..4270
                     /gene="frdB"
                     /locus_tag="OO327_003287"
     CDS             3536..4270
                     /gene="frdB"
                     /locus_tag="OO327_003287"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR6019715.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4281..4676
                     /gene="frdC"
                     /locus_tag="OO327_003288"
     CDS             4281..4676
                     /gene="frdC"
                     /locus_tag="OO327_003288"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR6019716.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4687..5046
                     /gene="frdD"
                     /locus_tag="OO327_003289"
     CDS             4687..5046
                     /gene="frdD"
                     /locus_tag="OO327_003289"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6019717.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5109..6242
                     /locus_tag="OO327_003290"
     CDS             5109..6242
                     /locus_tag="OO327_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR6019718.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6331..6864
                     /gene="blc"
                     /locus_tag="OO327_003291"
     CDS             6331..6864
                     /gene="blc"
                     /locus_tag="OO327_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6019719.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6861..7178)
                     /gene="sugE"
                     /locus_tag="OO327_003292"
     CDS             complement(6861..7178)
                     /gene="sugE"
                     /locus_tag="OO327_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR6019720.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7354..7500)
                     /gene="ecnB"
                     /locus_tag="OO327_003293"
     CDS             complement(7354..7500)
                     /gene="ecnB"
                     /locus_tag="OO327_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR6019721.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7611..7736)
                     /gene="ecnA"
                     /locus_tag="OO327_003294"
     CDS             complement(7611..7736)
                     /gene="ecnA"
                     /locus_tag="OO327_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR6019722.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7788..8354)
                     /gene="efp"
                     /locus_tag="OO327_003295"
     CDS             complement(7788..8354)
                     /gene="efp"
                     /locus_tag="OO327_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR6019723.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8396..9424
                     /gene="epmB"
                     /locus_tag="OO327_003296"
     CDS             8396..9424
                     /gene="epmB"
                     /locus_tag="OO327_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR6019724.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2465 a         2480 c         2466 g         2269 t
ORIGIN      
        1 ggcattatct gaacataaaa cactatcagt aagttggagt cattaccatt aaggcgaatg
       61 gaataaagaa taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt
      121 aataagccga tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc
      181 ctatcagact catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg
      241 tataacacgt gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat
      301 acagaattga tacttggatg atatcaaata tccagtctta aattgtggcc tttaacggaa
      361 aaatgaaaac atatgctgca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt
      421 aatttttcag aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc
      481 gcccagcgcc aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat
      541 accgactttc aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc
      601 cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc
      661 atggaaacca ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg
      721 atgatcttcg gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa
      781 ggtcggtttt tctttaccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa
      841 cgtgtcgcgg tcttcttcgg tatcagcaac attgctcaaa tccagtttcg ctgcgacttc
      901 ccgcagttgc gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg
      961 ttgataagaa aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac
     1021 ctcgttcatc aaccggtaca tatcatagtg cggtcgatac cactccagca tagtgaactc
     1081 agggttgtga taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg
     1141 cccacaacca gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag
     1201 attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat
     1261 atcggttacc gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc
     1321 aaagaaacga cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc
     1381 gctcggctgc caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac
     1441 gaagtctact cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg
     1501 cagtgactaa aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat
     1561 aatttagccc tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca
     1621 gactatactg ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca
     1681 ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa
     1741 acctttcaag ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt
     1801 gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg
     1861 cgtagccata ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc
     1921 ttcgaatatc actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc
     1981 gtggattatt tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtggggatgc
     2041 ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc
     2101 gagcgcacct ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag
     2161 acctctctgc aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg
     2221 gttgatgatg gtcatgttcg cggcctggta gcaataaaca tgatggaagg cacgttggtg
     2281 cagatccgtg ctaacgcggt cgttatggct accggcggtg cgggtcgcgt ttatcgttac
     2341 aacaccaacg gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt
     2401 ccgctgcgtg acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc
     2461 ctgatgaccg aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt
     2521 tatctgcaag attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat
     2581 atggaactgg gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc
     2641 aacaccatct ccacgccgcg tggtgatgtg gtttatctcg acctgcgtca cctcggcgag
     2701 aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc
     2761 gatccggtta aagaaccgat tccggtacgt ccgactgcac actacaccat gggcggtatc
     2821 gaaaccgatc agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc
     2881 tctgttggtc tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc
     2941 ttcggccgtc tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac
     3001 gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctagttaac
     3061 caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa
     3121 ggttgcggta tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag
     3181 ctgcaggaac gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac
     3241 ctgctctaca ccattgaact gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc
     3301 gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag
     3361 cgtgacgacg tcaactttct caaacacacc ctcgccttcc gcgatgctga tggcacgact
     3421 cgcctggagt acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt
     3481 ggcgaagcgg atgcagccga taaggcggaa gcagccaata ataaggagaa ggcgaatggc
     3541 tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc
     3601 gcatagcgca ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg
     3661 ctacatcaaa gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat
     3721 ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct
     3781 gcgtgattac accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga
     3841 tctggtggtc gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat
     3901 cggcaactcc cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa
     3961 gtatcaccag ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt
     4021 tggcctgaac ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga
     4081 agatagccgc gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggtgt
     4141 atggagctgt actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc
     4201 tgcggccatt cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa
     4261 accacgctaa ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt
     4321 ccacctggtg gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg
     4381 ttccggctgt gtggttcagc attgaactga ttttcgcgct gtttgccctg aaaaatggcc
     4441 cggaagcctg ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc
     4501 tgatcactct gacggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag
     4561 cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct
     4621 gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag
     4681 cctgagatga ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc
     4741 ggggccggtg gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt
     4801 ctgctgccac tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc
     4861 gcgcagagct tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt
     4921 ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa
     4981 tgggttttct acggtctggc tgctatcctg acagttgtca cgctgattgg tgtcgttaca
     5041 atctaacgca tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac
     5101 cagaccttat gttcaaaacg acgctctgcg ccttattaat taccgcctct tactccacat
     5161 ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc
     5221 aacaaaagat ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta
     5281 cctggggcta tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt
     5341 taggttcggt cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg
     5401 aaatcaagtt aagcgatccc gcaacaaaat actggcctga acttaccgct aaacagtgga
     5461 atgggatcac actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc
     5521 cggatgaagt gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat
     5581 gggctccagg aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg
     5641 ctgtgaagcc gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac
     5701 tcaaactcaa ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg
     5761 gatatcgcga aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg
     5821 gtgtgaagtc gaccattgaa gatatggccc gctgggtgca aagcaattta cccccccttg
     5881 atatcaatga gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa
     5941 ccggcgatat gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca
     6001 gcatcattaa cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta
     6061 agcccccaac tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat
     6121 ttggtagcta tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca
     6181 aaaactatcc caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt
     6241 aaaatttcat cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac
     6301 acttagaaaa aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg
     6361 acagctgcat ttctggtcgt tgcctgtagt tttcctacgc cgccgcgtgg cgtgaccgta
     6421 gtgaataatt tcgacgccaa acgttatctc ggtacctggt atgagattgc ccgttttgat
     6481 caccgttttg aacgtggact ggaaaaagtc accgcaacat acagcctgcg tgatgacggc
     6541 ggcctgaatg tcattaataa aggctataac cctgacagag gaatgtggca gcagagtgaa
     6601 gggaaagcgt actttaccgg cgcaccaact cgcgctgcgc tgaaagtgtc attctttggt
     6661 cctttctatg gcggttataa cgttattgca ctcgatcggg aataccgcca tgcgctggtt
     6721 tgcggcccgg accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa
     6781 gtgaaacagg agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt
     6841 tgggtacagc agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg
     6901 attaacgcca gactcgccag acgcatcggg ttagcggact caccgagcag cacaatgccg
     6961 gtgatggccg cgccgactgc gccaataccc gtccacacgg cataagccgt ccctaccggt
     7021 aacgatttca tcgcccaggc aagtagcgcc atactgacta tcatcgccgt cacggtaata
     7081 acactcggcg tcaaacggct aaagccgtgg gtatatttca ggccaacggc ccataccact
     7141 tccagcagac cagcaataac taagataatc caggacatat caggctccgg aaatttgggg
     7201 ccgtccccgg tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat
     7261 attttgccag gtggcagggg aatttcaact attaccttta tgacgatcgc agaaaaaaca
     7321 aacggcacga cactgctgtc gtaccgttat tgcttattgc tgcgctttcg ttgctgcgcc
     7381 agaaatcgcg ttaccgccat cagaaatgtc ttcaccaacg ccacgcgtgg tgttgcaggc
     7441 agttaatact gttgaaagca ccagaacaga aaagatcgct gcaattgtct tcttcaccat
     7501 aacgtcttcc ttttaggtaa tgttatttat gtttgcctat agcaaattaa taatagacaa
     7561 catcgccaaa aatgatggaa atctgatgat ttttcggaag agaagaaaaa ttagctggca
     7621 gcgcgagaga tggagttacc gagatgtttg atgtcttcgc cgaaaccgca agcggtgtta
     7681 cagcccgtga gcagcgtgct ggcaagcaaa acaagaacga taaggcgttt catcatctct
     7741 gacctgaata agtgatggtg cagcctgcag gccgcaccac aaccgcatta cttcacgcga
     7801 gagacgtatt caccagagcg ggtatccact ttgatgactt cgccgatttg tacgaacagc
     7861 ggaactttaa ccacagcgcc agtagacagg gtagccggtt tgccgccagt acctgcggta
     7921 tcacctttca ggcccggatc ggtatcaacg atttccagtt caacgaagtt cggcggagta
     7981 acggagatcg gctgaccatt ccacagagtt acgatacact ctgcctgatc cagcagccat
     8041 ttagcattgt cgccaattgc ttttgcatca gcagacagct gctcgaaagt ttcgttgttc
     8101 atgaagtgcc agaactcacc gtcgttgtac aggtaagtca ggttcatatc gacaacatca
     8161 gcgccttcag cggaatcagt agatttgaag gttttttcta cgcgagtacc ggtcagcaga
     8221 cgacgcagtt taacgcgagc aaatgcctgg cctttacccg gttttacgaa ttcactcgct
     8281 tcaaccgcgt aaggttcgcc gtctaacatg attttaagac cagcacgaaa atcgttgcta
     8341 tagtacgttg ccataaggcc ctctgaaatt tgttaattgg tagctaagcc acaaaatggc
     8401 gcatattgta accctaaata ccccatccag agaagattgg ttaacgcaac ttgccgatgt
     8461 tgtgaccgat cctgatgaac ttctgcgtct tttgaatata gacgcggacg aaaaactgtt
     8521 ggccggacgc agcgccaaaa agctgtttgc cctgcgtgtg ccccgctcat ttatcgatcg
     8581 catggagaaa ggcaatccgg acgatcctct tttgcgtcag gtacttacct cgcaagatga
     8641 gtttgtcgtc gcgtccggat tctccaccga cccactggaa gaacagcaca gcgtagtgcc
     8701 tggtttgttg cataaatacc acaaccgggc gcttttgctg gtcaaaggcg gctgcgcggt
     8761 aaattgccgc tattgcttcc gtcgccactt cccctatgcc gaaaatcagg gcaacaagcg
     8821 taactggcag actgcacttg agtatgttgc tgcgcatccg gaactggacg agatgatttt
     8881 ctccggcggc gatccgctga tggcgaaaga gcacgagctg gactggttgc tcacacaact
     8941 ggaagccatc ccgcatatca aacgtctgcg gattcacagc cgtctgccga ttgtgatccc
     9001 ggcacgtatc accgaggcgc tggttgaacg ctttgcccgt tctacgctgc aaatcttgct
     9061 ggtgaatcac atcaatcatg ccaatgaggt agatgaaacg ttccgtcagg cgatggctaa
     9121 attgcgccgt gtcggtgtca ccctgcttaa ccagagcgtt ctgttacgtg gtgtgaacga
     9181 taacgcacaa acgttggcaa acctgagtaa tgcgttgttc gatgccggcg tgatgccgta
     9241 ttacctgcat gtgctagata aagtacaggg cgcggcgcat tttatggtga gtgatgacga
     9301 agcacggcag attatgcgtg agttgctgac attggtgtcg ggatatctgg tgccgaaact
     9361 ggcgcgagaa attggcggcg aacccagcaa aacgccgctg gatctccagc tacgccagca
     9421 gtaactgatt aaacagggaa gttaaacatt aaaattgcct gatgcgctac gcttatcagg
     9481 cctacatcgt gcattgaaat tgaatggatt tgcaatgaac tgtaggccgg ataaggcgtt
     9541 tacgccgcat ccggcaagag caacaaacgc aagtacagca accactgaga aatttaatct
     9601 ttgcacgttg cataaccttt aattattcat atgcattgct gttgttactt tttatatagc
     9661 taacaccaat aattaacctt
//
LOCUS       DAJTSI010000145         9595 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000145 DAJTSI010000000
VERSION     DAJTSI010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9595
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.145"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(7..327)
                     /gene="psiF"
                     /locus_tag="OO327_003297"
     CDS             complement(7..327)
                     /gene="psiF"
                     /locus_tag="OO327_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR6019725.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(446..1861)
                     /gene="phoA"
                     /locus_tag="OO327_003298"
     CDS             complement(446..1861)
                     /gene="phoA"
                     /locus_tag="OO327_003298"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR6019726.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTDVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1962..2222)
                     /gene="iraP"
                     /locus_tag="OO327_003299"
     CDS             complement(1962..2222)
                     /gene="iraP"
                     /locus_tag="OO327_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR6019727.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2404..2607)
                     /locus_tag="OO327_003300"
     CDS             complement(2404..2607)
                     /locus_tag="OO327_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019728.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2685..3779
                     /gene="ddlA"
                     /locus_tag="OO327_003301"
     CDS             2685..3779
                     /gene="ddlA"
                     /locus_tag="OO327_003301"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6019729.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3803..4015)
                     /locus_tag="OO327_003302"
     CDS             complement(3803..4015)
                     /locus_tag="OO327_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR6019730.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4275..4583
                     /locus_tag="OO327_003303"
     CDS             4275..4583
                     /locus_tag="OO327_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR6019731.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4642..5736)
                     /gene="yaiW"
                     /locus_tag="OO327_003304"
     CDS             complement(4642..5736)
                     /gene="yaiW"
                     /locus_tag="OO327_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR6019732.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5749..6969)
                     /gene="sbmA"
                     /locus_tag="OO327_003305"
     CDS             complement(5749..6969)
                     /gene="sbmA"
                     /locus_tag="OO327_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR6019733.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7321..8478
                     /gene="ampH"
                     /locus_tag="OO327_003306"
     CDS             7321..8478
                     /gene="ampH"
                     /locus_tag="OO327_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR6019734.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8479..8982)
                     /locus_tag="OO327_003307"
     CDS             complement(8479..8982)
                     /locus_tag="OO327_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6019735.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(9070..>9595)
                     /locus_tag="OO327_003308"
     CDS             complement(9070..>9595)
                     /locus_tag="OO327_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6019736.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         2381 a         2448 c         2285 g         2481 t
ORIGIN      
        1 caggtatcag gcggctttct tgaggcaggc actcataaac ttattacgat catcaccttt
       61 cagagcttgt tgcgttgctt gattattaca ttcacgcatc ttttgctgct gtggcgtcaa
      121 acttttttca ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt aggtcttacg
      181 agcatcccct ttcaacgcct gcgccgtcgc ctgctgatta caggaggtca tacgctgttg
      241 ttgtggggtt aaggttctct cggcagcgcc gacggtggtt aaaaaaacca gaccaaaaag
      301 caaggtaacc agtaatgtta ttttcatagc accatccctc ttcatgtttt aaccatgagc
      361 gtttgcgccc gtgatctgcc attaagtctg gttgctaaca gcaaaaaaac cacccggcag
      421 cgaaaattca ctgccgggcg cagttttatt tcagccccag agcggctttc atggtgtaga
      481 agagatcggt ctggtcggtc agtccaacaa cattggcggc atgcgggcca tacgccgcaa
      541 tacgcaactg actgccggta tgttcttgtg aatcctcttc ggaattcccg taactcatca
      601 ccatcactgc gccatcttta gtatttagcg cctgagtgag gcccggcgct ttggtatccg
      661 gcgcaacaac ctggctggcg tgggcgtgat cagcggtgac tatgaccagc gtgttaccct
      721 cctttttagc gaattccagc gcccgttgta cggcttcatc gagatcgacc gtctcgccaa
      781 tttgcccaca agggttcgca gcgtgatcct gtttatcgat tgacgcacct tcaacttgca
      841 ggaaaaatcc tttctcattt ttactcaaca attcaatggc tttgtcggtc atctgtgcca
      901 gggttggtac actgtcatta cgttgcggat taggcgtata ggtgactgcg ggcttatcga
      961 tattgccgtg gtacgttgct ttcggcccta gccagcgcac tggcatattg ccgtcagcaa
     1021 acaggccaag caggggtttt tgctgattcg cttccgtcac cgaattcagt gaggcagcat
     1081 cgttcaccaa ctgataacca cgcgcctgtg cctgttcacg cagcgttttt ccctgccatt
     1141 caccagcggt tgccgtttca gcaaaggttt ttgcgccgcc gccaagcgta acgtcggcac
     1201 gagcgttaag cagctgttcg gtaatcgatc cttttccgcc tttttccaga gcgttacccg
     1261 gacatttttc actggtcgcg ctcggaccgt agcatttgcg cgaagtcaca tgtgccacca
     1321 gcgcagcggg cgtggcatcc tgcaactctg cggtagaaac gttaccggtc gccagacctg
     1381 cggcttttgc catttccaga atcgttgggt gatctttttc gtgaatatcg acgcccagcg
     1441 cgccgttata ggttttgaca tcggttgacc aggcggttgc tgatgcagcc gagtcggtga
     1501 cgtagtccgg tttgccagtt tttttattca gcgcatagtg agtgtattgc ccggtaagcg
     1561 gtaaggcatc gataccttta aaaaagccgc ccgcaccttc ggcataatta cgtgcggcag
     1621 taatttccga atcccccatc ccatcgccaa tcagcaaaat aatatttttt gcaggtttat
     1681 cgctaagaga atcacgcaga gcggcggtct gatcacccgt taagcggcga gcaccgccgg
     1741 gtgcagtaat atcgccctga gcagcccggt tttccagaac aggcatttct ggtgtccggg
     1801 cttttgtcac aggggtaaac agtaacggta agagtgccag tgcaatagtg ctttgtttca
     1861 ctttattttc tccatgtaca aatacattaa aaaataaaaa caaagcgact ataagtctcg
     1921 gccgtgacaa ctttatgaca gctgttgaaa agattaactt tttactgaca aggatgcttc
     1981 aataacttct ttacgtaatc gcgcagcagc tctgtatcgt cgtcaggaat gctggcatcg
     2041 ggctttacct cgtacagcgc cccctctacc tgatcaatca accgctgttg gtcattttgc
     2101 gccatattgc gaagcattgc agtgacgata atctccaaag cttctacctg cgcacacagt
     2161 tctttcgact cttcttcttt ttgggcaagc ttaaataaca actcagcaat gagatttttc
     2221 atgtctgtat ttccttatcc aaagtatgga gaagttaaca ttatgtttca caacttcata
     2281 gtgtctggaa gtattatttt tatgacaaag gaaatacttt gcgcaaatat tttttgtttg
     2341 attgccagcg aaacgtttct cttatttaaa taattaccca cacaaaatat aggcttttaa
     2401 ttatcaacct ttattataaa taaaagctat gaaatatatt ctcttcgtta acttataatt
     2461 aagagaaaaa aactcaccgc attgccattt aagccaggaa taaattagct tcccgttcat
     2521 catttttgtg atcttgcgca catctttgag ataacgcact ttcttcacca gtacgttgca
     2581 cgctttgtca gcagaagatt gcaacaatct ttcagaaggc cgcgaatccc ccgttttgtc
     2641 gtggtaatgt atgcactttg tgttagacgg gataggtttt taagatggaa aaactgcggg
     2701 taggaatcgt ttttggtggt aaatcagcgg aacatgaagt gtctctgcaa tcggcaaaaa
     2761 acattgtcga tgccattgat aaaagtcgct tcgacgttgt gctgctgggc attgataaac
     2821 aagggcaatg gcacgtcagc gatgccagca attatctgct aaatgcagac gatcctgccc
     2881 atattgcgtt gcgcccttcg gcgaccagcc ttgcgcaggt gccaggtaaa catgagcatc
     2941 agcttatcga cgcgcaaaac ggtcagccgt tgccgacggt ggatgtcatt ttcccgattg
     3001 tccacggtac gctgggcgaa gatggttcct tgcagggaat gctgcgggtc gccaatttac
     3061 cgtttgtagg ttctgatgtt ctggcttcag cagcctgtat ggataaagat gtcaccaaac
     3121 gtctgctacg cgatgccggg ctgaacattg cgccatttat taccctgacg cgcgctaatc
     3181 gtcacaacat cagttttgcc gaagtggagt ctaaactggg gttaccactg tttgtaaaac
     3241 cggctaatca gggctcttct gttggtgtca gcaaagtaac cagtgaagaa cagtacgcaa
     3301 ttgccgtcga tctggcgttc gagttcgatc ataaagtgat cgttgagcaa gggatcaaag
     3361 gtcgtgagat cgaatgcgca gttctgggca acgacaatcc gcaagccagc acctgtggcg
     3421 agatcgtact caccagcgat ttctatgcct acgacaccaa gtacattgac gaagatggcg
     3481 cgaaagtggt agttccggca gccattgcac cagagatcaa cgataagatc cgggcgattg
     3541 ccgttcaggc ttatcagacg ttgggatgcg caggcatggc gcgtgtagac gtgtttttaa
     3601 ccccagagaa cgaagtggtg atcaacgaga tcaacacact gcctggcttc accaacatca
     3661 gtatgtatcc gaagctgtgg caagccagcg gtctgggtta caccgatctg atcacacgtt
     3721 tgattgagct ggcgctggag cgtcacgctg cggataacgc attgaaaacc acaatgtaat
     3781 attttaacgc ccgataatat tcttattggg cgttttcagt ttcttcatcg cgacgacgaa
     3841 taatcaaccc cgccagccag aaactaatca cccacgtcac cagaccgacg gcataggtct
     3901 gccaaccttt tgcttcaaat cccagtaccc ccaccacgcc attaagaatg aatataaggc
     3961 caatggcgaa cgcatagtag tgccagtcac ggcggatttt tacaggcaga ttcatggcag
     4021 ctccgttaac gaaaaacaac atccttgcat attctgtaac aaccgtctgt ggtgtagaca
     4081 acattttcgt aattattaat aaatttaact taaggttgcc attttgtgac tcacaacgaa
     4141 gattcacgct tcgggaatat tcccaacatc aatttaccat gattctgata ttgacaatct
     4201 aaatgacctg tcagacttgc cataagccgc tggtatcaca ccagaagaga agcagatgac
     4261 ttccggaggt ttttatggct gatttcaccc tgtcaaaatc gctgtttagc ggaaaatatc
     4321 gcaatgcctc ttcaacgcct ggcaacattg cctatgcgtt gtttgtgctg ttttgctttt
     4381 gggctggggc gcaattgctg aacctgttag tgcatgcgcc cggcgtctat gagcgtttaa
     4441 tgcaggtcca ggaaacaggt cgcccacggg tggaaattgg tttaggtgtc ggcaccattt
     4501 tcgggctgat cccgttttta gcaggctgcc tcatttttgc agtggtggcg ctatggctgc
     4561 actggcgaca tcgccgccag taaaatttac gttgccagat gcggcctgaa acgccttatc
     4621 tggcctgaca ttcctgtttg atcactgttt catacaacgc gcccgccgtt cgtctacgcg
     4681 cttcgcaaac caggccgtag tcaggttgcg ggtgattttc gggctttcca gttgaatgcc
     4741 aggcaacatt tcgcggggta gtgatttacc cgttttcgct tcggcaagtt gataaacttt
     4801 cttgtacagc gccgtctcct caaaagagaa gctgtcacct ttctctaact gacggcgaat
     4861 ttcgctgtcg ctcatcccca gttttcccgc cagtttgcgc gttgccagtt cggttttccc
     4921 gggttcttta ctgtcgtaac gaattaaatc accgtccagt gccagcttca cgccgctggc
     4981 cttactgact gcgttctgaa acgcggcatt acggctggcg taccagccag cgttaaaatc
     5041 agcaaagcgg tatatcggtg cgctatagct ggcgggataa ttcagtaaat ggtaagtacc
     5101 aaaccacaac ccgccgcggc gactgaagac ttcctgacga actgtaccgt ccattttcca
     5161 cggataccct ttggtatgct gttcggcaaa agcaatgctg acctgcatcg gcccaccggt
     5221 gcgcaccgga ttaagcgaac caaacagcgt ctgccccatt ggtaccatgc tgatcagatc
     5281 gtcaaaaatc gcgctcaatt gcttttctgt acgcaccgaa tctaaccgtt cgctataact
     5341 tttgccgttt ggcgatttga tttttagcgc ggtatgaacc agaaaagcag gaatgtgcat
     5401 ccgttcagca cgacggtcaa tctcttgcca ggcgatcttg cttaagcccg gaacggccgg
     5461 atctgcctga taatttgact cctgctgcgc caccgccagc accgagtaga cgttttccag
     5521 cgtcggtgcc agcccctgac tttcaaaggt ggtggcgaga tctttcgccc aggcatcgcg
     5581 gtctttcacg ctcgcaggca tcttctgccg cacgacgctc gctacatcta tcgctttttc
     5641 gccctttttc aacggttgcg gagcctgact actacacccc gctaaaacca gaaccgccaa
     5701 caatgacagc gaagatagat gattgacgcg cgacattact tctccttttt agctcaaggt
     5761 atgggttact tcctgaattt tgtcaccatc cagctcatgt tcaaagctgc gcagacgttt
     5821 gtagatagac atcaactcaa ccagtgtggt ccatgagtta atcaggtact ggaaagcacc
     5881 gcgaacctga ccaaaaacgt tggtaatctg cgtcatcagg ccgagcgtaa tcgtaccggc
     5941 aacaattgac ggaaacagca agaacaaacc gaaaacgtta tcgacctgca aatagagaat
     6001 gcgggcgatg ttgaaataca tatagtgaaa atagaggcga aaatagtttt tccgtacggc
     6061 gctaaacagc tcgcgtaccg taggcggcgt cgcgcgcgtg gcatcgtctt caccataaac
     6121 cagctcttta cggtaggcgg cctctactcg ctggttttta aactccagcc ccggcagttt
     6181 gatccctacc actgccagca atccggtccc catcagcgac cagacgatag cggcaatcac
     6241 cagaccatac ggaatgtgcc cgataatcgg cagctccggc acatgcgcgg agagcgttac
     6301 cagcaccggc aggaaggcga tcaacgtcat gatggcgttg ataaaactga cgcccatatt
     6361 ctccagcgtt gaagcaaaac gcatggtgtc ttcctgcaca cgctgtgcgg caccttcgat
     6421 atgacgcagt tgttgccagt tcgccatgta atattcgttc atcgccgtac gccagcggaa
     6481 cacgtagtga ctgacaaaga agttgttcag tacactgatc accaccgcga tcagcgcaat
     6541 ccccagaaag acgcccactt cgcggtaaaa ttgttcgata gtgactttat gcggcgaact
     6601 tagcgcggtt tgaatcagat catagaacgg cgcataccag gcgttgacgg cgaccccgac
     6661 ttccaccaaa aaccaagtga cgaagataat cagcgcagta ccgagtattg accagtattg
     6721 ccaacggtgc gggctgtaga taaaccagaa aaatgcaaaa agtcctacgc aaacaatgta
     6781 gtaagcgtaa aaaatcagga aatccaacga ccagaaacgc gcggcgctaa tcgggatctg
     6841 cccggaagcg ccggtgatac gtgccaccca gtcaccccca ccggcttgcc agaagataac
     6901 ggcaatcaat gcccaaacaa aggccgagag aaaaaacgtt cccggctttg ggaaaaaaga
     6961 cttaaacata tgcactcctg ctaacttctt atcgttttaa caactgctgt gtattaaccg
     7021 catgaccgct acaaagcgtc agccaaatga aaccgcccat tgtgacccat agcaaggcgc
     7081 ttagttcgcc agcctgacca taagaattgt ttcgacggat ttcacaaacc aaagattgtt
     7141 tacataagcg gattaacacg cgcaggatca caaatgcagg cattggtgag caggaaaaag
     7201 atcgtgttta cgcgccgtta cgtttttaac actattattg cactaattcg ccctttgcaa
     7261 tccatcaatg agtagtataa atacgctcag ttaccttcat tcaatctatg gacaccaccg
     7321 ttgaaacgta gtctgctttt ttctgccgtg ctgtgtgcgg cgtcattgac ctccgtccat
     7381 gcggcgcagc cgatcactga accggagttt gcctctgata ttgtcgatcg ttatgccgat
     7441 catatttttt acggcagcgg tgccacgggg atggcgctgg ttgttatcga cggtaaccag
     7501 cgcgtctttc gtagttatgg tgaaacgcga cctggtaata acgttcgccc gcagctggat
     7561 tcggtcgtgc gtattgcttc cctcaccaaa ctaatgacca gtgaaatgct ggtgaaattg
     7621 ctcgaccagg ggaccgtaaa gttaaacgat ccgctaagta aatatgctcc gccaggcgca
     7681 cgcgtgccga cctacaatgg gacgcccatt accctagtga atctggcaac ccataccagc
     7741 gccctgcccc gtgaacagcc cggtggcgcg gcacatcgtc cggtgtttgt ctggccaacg
     7801 cgcgagcaac gctggaaata cctttctacg gcgaagctga aagccgcgcc aggcagtcag
     7861 gcggcgtact ctaaccttgc gttcgacctg ctggcagatg cactggcgaa tgcgtccggc
     7921 aagccttata cccagttgtt tgaagagcag attacccatc cattggggat gaaagacacc
     7981 acctacaccc cctcaccgga tcagtgccgc cgtttaatgg ttgctgagcg cggtgccagt
     8041 ccgtgcaata acacgctggc ggcaattggc agcggcggcg tctattccac gcctggcgat
     8101 atgatgcgct ggatgcagca gtatttgtca tctgattttt atcagcgcag taaccaggca
     8161 gatcgcatgc agacgttgat ttatcagcgc gcgcagttta cgaaagtgat tggcatggat
     8221 gtccccggca aagcggatgc gcttggttta ggctgggtat atatggcccc gaaagagggt
     8281 cgtccgagga ttattcagaa gacaggcggt ggtggcggtt tcattaccta tatggcgatg
     8341 atcccgcaga agaatattgg cgcgtttgtg gtggtcactc gttcgccgtt gacgcgcttt
     8401 aaaaatatga gcgatggcat caatgatctg gtgaccgagc taagcgggaa taaaccgttg
     8461 gttatccccg catcctgatt aatactctga aggcaaacgg ttgatagcga ccagtttgcc
     8521 tttattcatt tcgatatagc cgcctttacg caaagcagcg agaacttctg cgacgacaga
     8581 acgcgatatg cgtgtacgtt gatggatata attcatcaca ccaatacgtg atcgcaattc
     8641 ttcattccag tcaatcattg ataataatgt tgcgcgggtt tgtttggctg gtagatgata
     8701 tcccgtacaa tttccttctg atattaattt gtatggtata tcgtttttca ttaaaccatc
     8761 agccagcccc ataatataag gagcctgggt aataccgata agtacgtttt cttctctacg
     8821 cagagagata actccttcta aaataacgaa agtatcttcg ttattgacat cactggaaaa
     8881 tataacttgc ttttcattat taaactcgaa gcgcgtaccg tatctggaca aacatttatc
     8941 gagcttacca aattcctgaa gaggtttaac tacagataac atttgcgcgt cctttgcagt
     9001 aatgcccgtc aaatccttga cgggcattat ttagattaaa ttaccagtat ttcttcggag
     9061 tgaagaatat taccaagtat atttaacacc cacgttcgcg gaccagtctt gatctacgtc
     9121 accaccaccg aggtagttag catcggtata ggcgctgaag ttcttggtga agctgaactg
     9181 agtacccaga ccaacacgta ccgcagaccc ttcagtaccg ttatcgatgg aatcaccgtt
     9241 cacatcgtta tcgttgttag agtcgtcgta gacgtaagcc agtttgaagt acggagtcag
     9301 agcctgatct tcgctgtagg tgaaggtata acctgcatct acacccagtt cataacgcat
     9361 gctgtcgtaa ggctgaccgt caactttcat gtcgttgctc agctggtagt catcaccaga
     9421 ctggaacaga ccagaaatgc tgccgtaagg agtcacgtaa ccagcatcac ccagtttgaa
     9481 gtcgtaaccg gttttcaaac cgaagcccca agcgtcggag ttggtgctac cgtcaacgta
     9541 agtaccgttg ctcatggttg cagacaggtc gttgttgaag tgagagtagc tcaag
//
LOCUS       DAJTSI010000146         9527 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000146 DAJTSI010000000
VERSION     DAJTSI010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9527
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.146"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(54..533)
                     /gene="xdhC"
                     /locus_tag="OO327_003309"
     CDS             complement(54..533)
                     /gene="xdhC"
                     /locus_tag="OO327_003309"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCR6019737.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(530..1408)
                     /gene="xdhB"
                     /locus_tag="OO327_003310"
     CDS             complement(530..1408)
                     /gene="xdhB"
                     /locus_tag="OO327_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCR6019738.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1419..3716)
                     /gene="xdhA"
                     /locus_tag="OO327_003311"
                     /pseudo
     CDS             complement(1419..3716)
                     /gene="xdhA"
                     /locus_tag="OO327_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
     gene            4131..4886
                     /locus_tag="OO327_003312"
     CDS             4131..4886
                     /locus_tag="OO327_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR6019739.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4965..5038
                     /locus_tag="OO327_003313"
     tRNA            4965..5038
                     /locus_tag="OO327_003313"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4997..4999,aa:Gly,seq:ccc)
     gene            5173..6783
                     /locus_tag="OO327_003314"
     CDS             5173..6783
                     /locus_tag="OO327_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6019740.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLNGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEAGTVFIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFNGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            6815..7422
                     /locus_tag="OO327_003315"
                     /pseudo
     CDS             6815..7422
                     /locus_tag="OO327_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8353..9099
                     /locus_tag="OO327_003316"
     CDS             8353..9099
                     /locus_tag="OO327_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6019741.1"
                     /translation="MSQQEISIINLDQLVSMTSVEIAELTGKEHKHVLRDIRNMVEEL
                     NGAKTEHCSTLSSELNGSKFGLVGEEVYKDAKGESRTMYRLDRKHTFILVAGYSVHLR
                     AKCYDHIQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHR
                     GLFRKNPISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKM
                     RGILIAFTGTDQQKQEHLLKLAQSGKVEGI"
BASE COUNT         2431 a         2359 c         2419 g         2318 t
ORIGIN      
        1 tgttaaacac ataacctcag tgaatccggc ctgaattcag gccggatatc cttttacttc
       61 gttttctcgc aatccagaac tgtatttaca atcatctgat accccgtgca gcgacaaaga
      121 tttcccgcca gtccgcgacg aatttccgta atggttaatg gcttctcgcg tggtttcgcc
      181 agcattgccg tggtagccat aatcaggcca ggcgtacaaa acccgcactg cactgtgccg
      241 gatttcgcat aagcctgctg aacatgagaa agttttccgc ctttcgcttc accttccagc
      301 gtgcggatct cttttccttc agcccaggcg gcaaggtata agcaactgtc tattgctgtg
      361 ccgtcgacca acaccgtaca ggcaccacat tcacccacgc agcacccttg tttgacactt
      421 agcagtcctt gttcgcggag taattccgag agcggcgtgc ctggtgcggc gtgaagctga
      481 aaaggcatcc cgttaatggt gcattcggtg gtaattgttt cgctgtgatt cattgcaatt
      541 ttccccccgc cgcggcgacg gcttcgctaa tcactttttt ggtcatcgtc tggatgagat
      601 gcagacgaaa ctctttactg gcccgccatg aagaacgcgg ggcgacatct tgcaggacag
      661 attcgctgat agcttccagc gtttgcaggt ttaatggcgc attttgtgca gtctgttcgg
      721 catgttggca gcgaatcggc gttggcgcgg caacaccaaa tgccaggcgt aattcgctga
      781 aattgccgtt atccagtcgg caatgtgcgg cgcagccaat cgttgaaata tccattgcgt
      841 cgcgcatggc atatttaaaa tgcgcgctgc ccgcgtgttc tttcggctgt ggcggaaaat
      901 gaaaggcgac gaggatttcg tcatgctcaa gagacacttt gcccggcccg gtgtgaaagc
      961 cattaatcgg gacgaaacga acaccgcgcg gggagtggat ctccagtttc gcgtcataaa
     1021 ttagcgttgg cgtggcagaa tctgcgctgg tggcaccgtt gcaaatattt ccaccgtagg
     1081 tagcgacgtt acggatctgc ggtccagcaa tgaacgtggc cgcagcacat aacgccggga
     1141 gatgacgttg agttatagga tcttctatta gctgggtaaa tgtcgttgca gagccgatac
     1201 gtagcgagcc atcttccgcc agcgtaattc cccgcagctc cgccagatta tgaatatcaa
     1261 caatatggcg ataacggtca ttgtgatggt ggagctgaat cagtacgtca gtgccaccgg
     1321 cgagcagttt ggcctgcggg ttgtcagcca gcaggttgat ggcatcggca agggttgctg
     1381 cgcgatggta agaagcaaaa tcaaacatga tgttatcctc aaatcaatcc tgccagatgg
     1441 aactcttcat ataaccgttt tggcgtcagc ggcagtgtat tgattgcaac accggtagcc
     1501 atcttcaccg cgttacgaat agcagcggca acaggaatta tgggtggctc acccagtgac
     1561 ttatgtccgt atgcggattg cggctcattg atttcgacga acgcgctttc cagttgtggc
     1621 agatccggca tggtcggcat tttgtaatcc agcagattgg ggttacggac cacgccgctt
     1681 ttagcatcga tgatcatctc ttcaaatagc gcccagccaa tgcccattcc cattccgccg
     1741 tgtacctgac cttctgccag cagtggatta agaatatgcc ctgaatcatg aacgttgagg
     1801 atgcggttga tggtgacttt gcacagcgca atatcgaccg tcagatcaac aaaggtacag
     1861 ccaaacgccg gtgggttagt ggtggttttg atggagcttt cagcagagag ctgcccgccg
     1921 cgttcagggt ggtagaaagc gtccatcgcc aaatctttta acgacattaa cggctcttcc
     1981 ggtcgttcaa ccagcacgat atggcctttt atcagggtca aattcatcgc tgactgatgt
     2041 agcatgactg cggcgtgagc gatgattttc tcttttaata atagtgccgc actgcgcagc
     2101 gcaggcgcgg caacatagct ctggcgtgag gcaaatgcgc cgggatcgaa cggcgtaacg
     2161 tcggtatctt gtgttgaaat aacgcgaacg tcgctgaccg gaacccccac ggtttctgcc
     2221 accatttgcg agaagacggt gtcggcaccc tgaccgattt ccgtcgcgcc gctttgcacg
     2281 ttgatggttc catcctgatt catcagaagg cgcgcgcctg ctatttctac gccgacaggc
     2341 caggtgttag aggtgtagct aaaacaggcg acgccaacgc cgcggcgcaa attgccttgc
     2401 tggttctggc attctgcacg gcgtttttcc cattcaaaga ttttccggcc tttttcaaga
     2461 cactccggca accctgcgct gcaaatacgt ttgcccgtga gcggattagc atctccttcg
     2521 cgggcggcgt tgcgtaaacg aatttcaaca ggatcaatac ctaacgctgt cgcggcgtca
     2581 tcaagcatag actcaacggc aaatacgact tgtggcgcgc cataaccacg catcgcacca
     2641 gccgagggga ggttggtata gcaggtcttt gaactgtaag cgtaggcaca acgaggataa
     2701 aggtaagcga ctttattccc ccccgcagaa gcgatggagt gcccgtgaga tgcataagcg
     2761 ccggtgttag acagaacatc cagactataa cctttcaagg ttccgtcgcg gttcacgccc
     2821 atttgcccgt caatagtaaa agcgtggcgg gtacgggttg cgaggaaaca ctcttcacgg
     2881 ctaagggaaa ctttcaccgg aatgccgcca agcttgctgg tcaggaatgc cgccattggc
     2941 tcttccagta catcctgttt attaccaaaa ccgccaccga caaacggttt gatgactcgt
     3001 acgcatgacc agggaatatc cagcgcctga ccaaccacgc ggcgaacaat gtgcgggatc
     3061 tgggtgctgg aaacgatggt aattcgcgag tcatcctcca tccacgccag cgatgttacg
     3121 ctttccatgt gacaatgttg aataacggga gtctgatagt gcccctgtac ctggtagtcg
     3181 gcggcatcga ttgtttgttg gacattgccc gtcgacatcg tgctttgttt cagtaaattg
     3241 ccgccgttat ggattggtgc agcgtcttct gtcagcgccg cttctggcgt ggtgataacg
     3301 ggtaattctt gccactcaat gctgaccaat tgcgccgctt tttctgccgt gagttcatcg
     3361 cgggccacga cgatggcaac ggcgtcacca tgatgacgaa catggcgagt tagcagtgcg
     3421 cgatcggcgg tatcgcgctt gttttcgtca agtgtctagg catgcccagc tgtagcgaat
     3481 ggaatatcag gcacatcttc ccaagtaaaa atcgccagta cgcccggtaa acttctggct
     3541 tgttcatcat taatacttac ggcataacca tgtgcgatag ggctacgtac atatttcgcg
     3601 taacacatgc ccgccataac ataatcgtca gtatatcgtg cccgcccggt gaccttagca
     3661 atggcatcga cgcgcatgca tgattcaccc gtagcggttg cttcccgcgc ttccatagaa
     3721 atcccctcga tttgaacatt tggttcttat ggcaaagatt tagtctaagc aggcaagatt
     3781 tacgccagaa acgacgataa gcgttagggg aggaaggaag tgtgatgacg ttaaaaaaac
     3841 acagatatga tacgaaacag gactctgaat agggacagtg atacgtatca atatgagtgg
     3901 cgataaaatc aatgtgataa atcaggatta ttacgcatca ggatgggaaa tagggtaaaa
     3961 agtcaggaac ttcgatacct ctacagcgaa tattttgctg atgatacttc cctttgccaa
     4021 atgaaattaa cctctttctc tattaccacg tttttccaga agcaagagat tgggtatcaa
     4081 gaggctggct gctatgatat ggcgtcttgt tttttaagcg aggaaagatt ttgagtgcgg
     4141 gacgcctgaa taaaaaatct ctgggtatcg tgatgttttt atcggttgga ctgctattgg
     4201 cgggctgttc gggtagcaaa tcatccgata caggaacgta ttccggctcc gtttacaccg
     4261 tgaaacgggg ggatacgcta tatcgtattt cgcgcaccac gggaaccagc gtaaaagagc
     4321 tggcgcgact gaacggcatt tccccccctt acaccattga agttggtcag aaactaaaac
     4381 tgggtggggc gaaaagtagc agtagtacac gtaaatcaac cgccaaatca acgactaaaa
     4441 ccgcatcggt tacaccgtca tcagcggtac cgaaatcttc ctggccgcca gtagggcaac
     4501 gttgttggtt atggccaacg acagggaaag ttatcatgcc gtattcgaca gcagatggcg
     4561 gcaataaagg gattgatatc tcagctccac ggggtacacc tatttacgcc gcgggtgcag
     4621 gaaaggtggt gtatgtgggc aaccagctgc gtggctacgg taatctcatc atgattaaac
     4681 acagtgaaga ttacattacg gcttacgccc ataatgacac gatgctggta aataatgggc
     4741 aaagcgtgaa ggctgggcaa aaaatcgcca ctatggggag cacggatgcg gcatctgttc
     4801 gcctgcattt ccagattcgt taccgtgcaa cggcaattga tccgctacgt tacttgccgc
     4861 cgcagggcag caagccaaaa tgctgatggc gaattaatca gcagtcagca gcgcggcact
     4921 tgctaaggag agcgtaaggt ttataatgcc ttacgcatct cgaagcgggc gtagttcaat
     4981 ggtagaacga gagcttccca agctctatac gagggttcga ttcccttcgc ccgctccatg
     5041 acacttctta aagtctacta acttactgaa tcctaaggga tacggcttaa cgccttccga
     5101 tagttttact cactgtaacc aacactgtga ctaactttgt tgaagtagtg agcagaccta
     5161 aggtctcagt acatgctcaa gtcaagaacc tacctttatc agcgtaatgg tgtcttttac
     5221 atccgtctta ggatgaagac cactagtcgc ctgaccgctt cgcttccttc tcacaaccgt
     5281 tataaactag cgtcagtatc tttacggacc aaggatagac gtaccgctat ggctcactca
     5341 cggcacatca agtcagcact taaagcaatc catgcagaca accctaacgc ctcttatgag
     5401 gagctacggg agcacctgaa gaccattgtc gagtgggaac ttagtgtcag ccgtgatgac
     5461 ctgaacgacc cggagtccta ccagctttac gttgaccagt acgatgacat taagtccaac
     5521 cttcgggaag ctgtagcgac cgagcgtctg actgtagacc agcatcgcta tattaatgac
     5581 gttatcggtg tgcttaaggc ctgtcaggat agactcaatg gcgatagctc tggcctattg
     5641 tcttaccttg agcctgagac tggcagtcta agaccgtccg tctccctatc tgtattggcg
     5701 gaacctgaag tccctgaacc taaagcactg accctcgcgt ctctcattga gcagtacgag
     5761 caggagaacg cccagaactg gaagccagcg accctaagcg aaaaccgagc gtcacactct
     5821 acgctaatcg agatattcga ctatctggat attcaggatg tcggcaaggc gacccgtgca
     5881 gatatgctca gagttcgtga agtcctccag cagctaccga agaaccgcaa gcaacgcttt
     5941 aagtctatgc cgctgtctga cttgctgaac cgggagtcta agactgactg tctggacgtc
     6001 gttaccatta acaataaata tcttatcaag atggctgctg tgtttaagtg ggcggtacgc
     6061 aacgacttga tagctaagaa cctgaccgaa ggtcttgagc tgaaagtccc tcagcgtaaa
     6121 gcctccgacg ctcgtgatgc gttcagtcct gagcaggtag ggcaactact ggtcgcagct
     6181 aaagcgtact cgcagaagac ctcaggtaag ccataccatt attacgtcac cgctctggct
     6241 gcgattacag gtgcgagact taacgaggtg gcccaacttc aggttaaaga tgtcaggacc
     6301 actgaggctg ggaccgtttt tatccatatc aacgaagacg atagcagcct gccgggtaag
     6361 agtatcaaga acgcccacag tgaccgctgt gtcccgttgg tagatggtgc ttatggtttt
     6421 gtattggctg actttatgtc actcgtggaa gaccgtagaa agactgaggg tgataatgct
     6481 atggtgttca atggcctcaa gctaatgaag aacggctacg gtgagcaggt tagcaagtgg
     6541 tttaaccgaa cgttgctgcc caaagtcctc gctgaccgta gcggcttagc ttttcactcg
     6601 tttagacata cagttgcgac ccagttaaaa caacatgggg tcgagttagc gtatgctcag
     6661 gcgattatgg gtcatagctc agggtctata acttacgacc gttacgcgaa agaggtcgag
     6721 gtggaaacgc tgaaagaaaa attagcggag tcattgtcag taaaaaaaat agacggaaag
     6781 taatcgcata gggatagcgg aatgtgagga cgtaatggac aaagaatttt tagttagcaa
     6841 agaaaataag ccttcatatg aatgtatgaa tactgctgaa acttgtttgg ggctggctgg
     6901 aatggcttcg gaagattggc aacgtgagca tcattgccgc acaggtataa tatttactgc
     6961 ttttgctatt gaggcaatgt tcatcttcta tagaaaacag gttgaccccg gctacgataa
     7021 gacgcagaaa gagtgcagaa agacaatgca caagaatacg ttaaaattgt gcggtataaa
     7081 caattatatg gggacaaaac cttaccaaat tatcaaagag tgtcttgagg taagagacgc
     7141 tattgctcac ggcgattcgt atacttcgag ctttaatttc tcggcggatc atttggataa
     7201 ccaagacgat attgcaaaag tgtcctggcg gtaaactctg aacaatttcg gggattaact
     7261 gttgagcgcc ttgcaaaata cattgaaatg gcaaagcata ttgaccatga gatttctgat
     7321 aatggttaca ggccttctga aggttacctt ccagcagccg agcgcagaag tctgatgtta
     7381 gcttttggcg tcagtggtgt gtcaatgtgg cccagtgctt gagcgataat acacctcact
     7441 atagaggaat ggacccttaa ctatcactat aggggagaga ccctaagtcc ccaccttaat
     7501 agcagtcacg acccatacac acattcatta agtctcaggc ttccggtagc ggaccgattg
     7561 gaaaccatcc gcagacgcct agtgttaaat caccttggcc tgagaccatt ccttttaacg
     7621 taagctatct tcccttttga caggcgggga gcagtagacc acatccgact ttaagttggc
     7681 tctgtgataa tcgctgcgcg gataactgaa gaaccaagac accattactg aggtctgcca
     7741 tccatttgtg gacaggcgga aactcaaaga ggttagatca gtggtgtctg aggttggagc
     7801 tttgagtctt gaccagcagg ctggaggaga ccatgagttt atcttgttat ggattgtgtt
     7861 atcacaattc caataagaag aactttaagt ccagcctcca ctcccgccaa cccgaagtaa
     7921 aattatggtc gctatgatgt ggtctccatg cttcgggtct tactggaagt actgaacact
     7981 ttcgcgtcaa gttctccgaa gttcattcct gtttgactaa actatcacta tagggaccat
     8041 atctcgcctc tgacctcctg agagacggca agcgagaaac cgtggtccac gctagcaagc
     8101 tagccaccag cctaaggcaa gacagcctct ggtcagcctg tgtgttcttt ggtctcgtga
     8161 cttaagtcta tcaccacaca cgctcggaca tgctgtcgaa ccttcatctc tatggtcatc
     8221 gcagaccact aaccccaatt acctcaagtc attctgtttg cgtcctgttg cctgctggtc
     8281 tccctttacg tgggggcttg agtaggtcag tggggtgcga cttcagggtg caaacaaaag
     8341 gagtacacgt aaatgtccca acaagaaatc agcatcatta acctcgacca gcttgtgtcc
     8401 atgacctcag ttgaaattgc tgagctgaca ggaaaagaac ataaacacgt tttacgagat
     8461 attcgtaata tggtcgaaga gcttaacggg gccaaaactg agcactgctc aactttgagc
     8521 agcgagctta acgggtccaa atttgggctc gttggcgagg aagtatacaa ggacgcgaaa
     8581 ggtgagagcc gaacaatgta tcgtctcgac cgtaagcata ctttcattct ggtcgctggc
     8641 tattccgttc acctccgcgc caagtgttac gaccatattc agacgttaga gcgtcgagtc
     8701 cttcaactgg aagaccagaa gaagcgagca gccattcagt ccgctaaccg ccgaggcgtg
     8761 acttggggcg actactgcaa aacctatggt ctgcccgctc agaagctaat gaccgcactt
     8821 ctccagcatc gtggcctctt tcgtaagaac ccaatcagta acgagtggtc tgtaaatccg
     8881 aagtattccg actgctttcg tatcattaag ccgtcagacc agaagttctc agcaggtggg
     8941 tataacttcc ggttcaacgc taaggggctg gaggtcttcg gtaagcctga gatggttgac
     9001 aagatgcgag gcatccttat tgcattcact ggcaccgacc agcagaagca agagcacctc
     9061 ctgaagctgg cacaatccgg taaggtggag ggaatctaag atgatgattg acccgcgcac
     9121 acctgagggg cgcatgactc tccgttaccg tggctaccgg actgaagtcc tcctcagaga
     9181 gcttggtcta gacccggaag atgagacccg ccagcaccaa tcccgagacg agctaatagc
     9241 ccaactggtc gctatgaaac tgccgcttaa ccgttagtag ctcacctact caatacctaa
     9301 tttaaacacc ttttgagatg accgatgcgg ggagacccgt aggccttact gtgcctgcga
     9361 taccgatagt ctcaactctc aagaggagaa ttacctatga tgttcaacaa caacaactgg
     9421 aagctgtcag tcaccgacat caacctttat gaaaacactg tgagtctgga cggtcagtcc
     9481 tatccgttgt cgctcgctat caagaccctc attccgggct acctgtc
//
LOCUS       DAJTSI010000147         9522 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000147 DAJTSI010000000
VERSION     DAJTSI010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9522
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.147"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..593)
                     /locus_tag="OO327_003317"
     CDS             complement(<1..593)
                     /locus_tag="OO327_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HCR6019742.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQ"
     gene            complement(660..1385)
                     /gene="fimC"
                     /locus_tag="OO327_003318"
     CDS             complement(660..1385)
                     /gene="fimC"
                     /locus_tag="OO327_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR6019743.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(1422..1961)
                     /locus_tag="OO327_003319"
     CDS             complement(1422..1961)
                     /locus_tag="OO327_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6019744.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(2026..2574)
                     /gene="fimA"
                     /locus_tag="OO327_003320"
     CDS             complement(2026..2574)
                     /gene="fimA"
                     /locus_tag="OO327_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR6019745.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(3055..3651)
                     /gene="fimE"
                     /locus_tag="OO327_003321"
     CDS             complement(3055..3651)
                     /gene="fimE"
                     /locus_tag="OO327_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCR6019746.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(4129..4731)
                     /gene="fimB"
                     /locus_tag="OO327_003322"
                     /pseudo
     CDS             complement(4129..4731)
                     /gene="fimB"
                     /locus_tag="OO327_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            6185..6901
                     /gene="nanC"
                     /locus_tag="OO327_003323"
     CDS             6185..6901
                     /gene="nanC"
                     /locus_tag="OO327_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR6019747.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            6921..8027
                     /gene="nanM"
                     /locus_tag="OO327_003324"
     CDS             6921..8027
                     /gene="nanM"
                     /locus_tag="OO327_003324"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR6019748.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATTAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            8092..9072
                     /locus_tag="OO327_003325"
     CDS             8092..9072
                     /locus_tag="OO327_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR6019749.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         2788 a         2077 c         1996 g         2661 t
ORIGIN      
        1 ttctgtacac tatttccgct gaaattataa ttgagcaatc cggcattaat accgggatcc
       61 cataactcag gaggaatata accacgcgcg cgattactca taaatgcctg agggatcgtc
      121 aggttcagtc gctgctgacc aacatccaga tgcgcagtag cgtcctggac cattgtggtt
      181 aatggcacac aggcatcatc cgccagcaga ttcataccgg cgacagaagc cgtattcagc
      241 cccatactgg cgagttgcgc gcgtgtcagg cagggaacaa tcccttgttc actgtcgccc
      301 gtattaaatg tgacatcacg cgttgccata taaccattat tcaaatagat atcgacgcga
      361 tacgtccctg gcggtaattc ttgcccattt tcaaaacgcg ataaatcggc cacagcctgg
      421 ggatcatccg ctaaaaagcg cagattaaaa tagaggtcgg cagatgacaa aggtgcctgt
      481 gcggcaaaag cacaggcgac aacgagtcgg acaaaaaaac cagccaaacg atgcttacga
      541 atatgcaagc attgtgtgtt tcgctggtaa agtcttaaat tcagatatga cattattcct
      601 ccggtttaaa atcacccggc agtcaattct ttttattcag gcgaaaaatt ccccctgcgt
      661 tattccatta cgcccgtcat tttgggggta agtgcgccat aatcatttat tgttcggtaa
      721 gtaatattgc ttcctgcatc agaaggcaat ttaaccgtgc tttcgcccat tggaggcacc
      781 aatgcatttt caagaacccg ggttccggca ttcaactctg ttaccgtcag gtaatagggt
      841 gtcgggttaa tcagcgtcag agaattcgcg ctacgacgaa atcttaattt ttctgcggcc
      901 tgatcgggtg gcaacgctaa tttagccggg cgatagtaca gtttaatgcg gctgataatt
      961 gcgagctgta gcatattctc agtcaatttt gatttatcca ttgacggaat cgctttaacg
     1021 ttcatccaga ataaactttc ccggtcctgt ggcaattggt tatttgttgc atcaagaata
     1081 cgtaaggtat tctctttttt tcccttcatc gcaaacagag gaggcgtcac gataaaacga
     1141 ccatccttta caccatcggc attttccacc catgattgaa ttaaataggt actattttca
     1201 tcattatttg tcacggcaag ttgcacttgt ttttgccctg ccggataaat tacgcgagtc
     1261 gcacctaagg ccactcctgc ttcagcgcgt ccggcaacca tcattgccat gaacatcagg
     1321 atacctgcca gcaagcagaa tgttatttcc tgcgatttcc ttacattgac gtttttatta
     1381 ctcactgtcc tgttcctgtt atcacattat ctactgaaca attactgata ggttaaagag
     1441 aaccaggcct gggcattggc gatgccgcca gtaacccgac gcccggtagc acgatatttg
     1501 gcgatgaaat gtagcgaagt agagcctgag taaagccgtt tccagtttgc tggaggacga
     1561 ttaatcggta cgaggtttcc ttcatcatca aacaacgcta caccaatatt ggaggctatc
     1621 cctggcccct ctcccacgga gagcacatcc ggatttttac catccgcgac accgtgaaac
     1681 gccacaccca cacgttcact caccaccgtg ctacattccc gtaaatgaat aacaaaaggc
     1741 accggtgcgc tatcttcccc tgccgcatga aaccggttac tgctgatttg ccccatattg
     1801 accgtcattt gtttatcacc ggcttcaatc cggcaagttt ccgcaataat gacgccctga
     1861 aattgcatat ttccgccggg cagcgtggta ttccatttat ttccggccag agcaaacatt
     1921 ggcagcaacg acgctaacag aaataatctc ttccttttca tcgttatttt tatcgcacaa
     1981 gggtgggcat ccctgcccgt aatgacgtcc ttgaacctgg gtaggttatt gatactgaac
     2041 cttgaaggtt gcatccgcat tagctgcacc cggggttgcc tcgccgattg cataataacg
     2101 cgcctggaac ggaatggtgt tggtaccgtt attcagggtt gtttgtgcac taaatgtcgc
     2161 accgtccagc gtcagagcat tgcctgttct gtctaggatc tgcacgccaa cgtttgttgc
     2221 actacctgca gcagaactct gcagagccag tacatcagta cgcgtagcat caattgccgt
     2281 acctaagaag gcaacagcgg cttttgtggc aacagtggta tcgcaatcat tcagctgaat
     2341 gttaaaacca acggcagagc tggttgctcc agcctgcttc aggctagcgg tacgaacctg
     2401 tcccaactga acggtttgat caacagagcc tgcatcaact gcgcaagcgg cgttaacaac
     2461 ttccccttta aagtgaatgg tcccaccatt taccgtcgta gtatcggcca gagccgctgc
     2521 ggaactgagg gacagagccg acagaacaac gattgccaga gttttaattt tcatgctgct
     2581 ttcctttaaa aaaactattt ctaaatcgac atgggcagtc gttctgtaca ctttgttttg
     2641 tcaacgagtt tgactgccaa cactgcacag ttttccccca aaagatgaaa catttggggc
     2701 cattttgact catagaggaa agcatcgcgc acaaactttt tcagtttatt tgttggctta
     2761 atattctatt attatcttta tttatagatt tttatattgc atgaggtggt ttttggagag
     2821 aagaatgagg aagatgcgtc gagccacaga aacgttagct ttacatatag cggaggtgat
     2881 gtgaaattaa tttacaagag aaataattta catatcaaat agttagatgc tttttgtcgt
     2941 tttttaatat ttttatgctt gagaaaaaat acgtaactta tttatgatat ggacagtttg
     3001 gccccaattg tcttgtgttt atttgttttt taactttatt atcaataagt taaatcaaac
     3061 ctcttctctt tttaattttt cgtttatgag attatttctt tcccataatc cggcaaaacg
     3121 agcagcatta ctggcggtat aacgcacagt atggcgaata tttcgatgcc cgagataatc
     3181 ctgaattaaa cgggtatctg caccacgctc cgccagttca taaccgcaag catgccttaa
     3241 catatgagga tgagtctgcg ttacggttcc agcttcaata ccggcatcgc gaataatgcg
     3301 ataggcctgc tggcgagaaa gccgactccc gcggcgagaa ataaatatag cgtcggtccg
     3361 gtcagcgcct ttccagttag cacgttcctg ggtccagcgt tccacggctt cacgctcatc
     3421 aaaacgtaac gggtgaacgg tagaaaatcc gttcttcagt cggcgaatat ttattcgacc
     3481 ttcattaagg tcaaggtcct gataatgcag atcaagcagt tcactaatac gcatcccatg
     3541 ccgatatgcc aacagaataa gacaataatc tctggctccc gttgccccgt aacaaaccgc
     3601 ctgcatcatg gcctgaactt ctttaccggt aagataacga cgtttactca caatagtagt
     3661 actcctaact gagatatatt tgaatgaata cctataggaa acctcaatcg gtcaaaatta
     3721 gcccaataga taaaaagata accacacacg aagttatgtg gctatcagtc aattacatta
     3781 aacatcatac aaaaataaaa tatcactgat gtgacaacat ctattttcat tgatttgatt
     3841 caatataaca atagattaca gaggattaac ttccagttac ttaaaaaata aagaaaaaag
     3901 tgtggaaaca ggagaattta agtgatgtgt aaacttatga atgattttgt ttatttattt
     3961 tgaagaacat aaatcattag attttgtatg ggtatggtta acttaatgtt tattatgata
     4021 attaagaatc acacttacaa taacatcaat taataaatgg ttaatcattt ttgatatatc
     4081 gtaagaataa tgtagttttt aacaccatcc ctggtatctc aactatctct ataaaacagc
     4141 atgacgctgt cgtcctctgg ctctatccca gatgccgtaa aaacgccctg cattgctggc
     4201 ggtataccag acagtatgac gaatattgcg atgcccaaga taatcctaga taagtcgcgt
     4261 atctattccc atattcgcca aagcaaaacc acacgaatag cgtaacatgt gcggatgaat
     4321 ctccagtgac aacccggcat taccaccaga agtcgagata atatggtaaa actgttgccg
     4381 agaaagcgga ttccccttac gtgataaaaa tacccactcg ctctcagcat gcggataaga
     4441 agtacggata ctcaaccagt tttttaaagc ctgaacttct ttgttcaata gcgggtgcgt
     4501 tgttgaaaag ccttttttta atcgatggat atatatacac tttgctttaa gatcaatatc
     4561 cgaaatcctc aatcgacaaa tttcactcgc ccggaaacca tgaataaaac aaagcaaaat
     4621 cagacaataa ttacgtgttg catgaggccc ggtatttgct gctttaagga gtgactcgat
     4681 ttcactatgg gtaaggaagt tccttttttt gttatcagcc ttattcctca tcgttttccc
     4741 ttataattac agacgcgcac tagctgtgct gggttagcaa taatccaaca tttttatcct
     4801 gattatgttt ataaaagcga acgtttgctt aataacttac gtaagtggac cagttcttct
     4861 gagcgaactt aaatggagta gcgatagtta cttataataa ctttgccata caaacgccat
     4921 gctcttgcat gctatgtacc tttatatatt tatcaatctg atacgaatct aataataacc
     4981 ctcatcatta atgcaactaa tcttatctat atatcatgtg atatgttctg taacaagtaa
     5041 aatcaacata aaaaattcag caaaatttaa tatttgttaa actagccgtc tcaaatatga
     5101 cattattagt taactgcatt tgccacaaaa tggctatttc atggaacgca gtaatttctt
     5161 agtggctatt atcatgctaa ttcttagatc cctagcgatt attcctgcct ggttcgtcag
     5221 cttaggccat gagattgtta cattcctttt tatcctggag atatggggga acgcaggcga
     5281 cgctttttca tctacatccg ttggtaaata tagctttggt cactacgact taagccttcc
     5341 tcaatcacag ctattcgccg ggcgatacgg caaacaaact aagtgaaaca tcatcctgaa
     5401 aacaccaaca tcaacaagcc tctccagatc gacatcagga gtgaccagtt acaagccaca
     5461 aaccaagaaa catatttcat gatgagaatt atgctcagcc tgatggcgag taaaccctta
     5521 ttttcaccac tctcactgag ttagcttgca aagctttctt tgtttagatt ctaaataaat
     5581 tatttataaa acgtatagtt aaaagaaaat ctttagtttt tgaagaccac cgcaagtgtt
     5641 cgtctggctt cacatggcat cttcttctta gaaaaagatc gacatatttt gtgacgcgaa
     5701 ttgcaaatct ggttttgttg tatggattgc gtgatttttg atctggtata acaggtataa
     5761 agatgcacca cgatagtcaa tgagactggg catctcgtaa tctatggcaa acatcacttc
     5821 agttctttca catcgggtga tgaaaatgca cttcagtctg aaaggaatat gaaaatgaga
     5881 tcaacagaca ctctatttta tgactctggg taaaatgggt tgagtaagtg atataactta
     5941 cgaatattca aatcaattaa aaatcagaag agattttata ctcaggtatt taatctggat
     6001 ctctgtttat ttaaataatg ttaaaagaga attttcacag gagaccttat acaaaaaata
     6061 taaaatacag ttacccgttg ccaaagacac tataagcctg gcaaaaaaat attacacaac
     6121 ataaatgcta attgtttatg cgggctttgt attgctttct gtatcctaca aatgagtgaa
     6181 atttatgaaa aaggctaaaa tactttctgg cgtattatta ctgtgctttt cgtccccatt
     6241 aatttctcag gctgcgacac tggacgtacg tggtggatat cgtagtggaa gccacgccta
     6301 tgagactcga ctcaaagtca gtgagggatg gcaaaatgga tggtgggcaa gcatggaaag
     6361 taatacctgg aataccattc atgataataa aaaggaaaat gccgcactca atgatgttca
     6421 ggttgaagtt aattacgcaa ttaaacttga tgatcaatgg acggtgcgcc cgggaatgtt
     6481 aacgcatttt agcagcaacg gcacacgcta cggaccctac gtaaaactgt cctgggacgc
     6541 gacaaaagat cttaattttg gcattcgcta tcgttacgac tggaaagctt accgacaaca
     6601 agacttatcc ggtgatatgt ctcgtgataa cgttcatcgt tgggatggat atgtcactta
     6661 ccatattaat agtgatttca ccttcgcatg gcaaacgacg ctatacagca aacagaatga
     6721 ttatcgctat gcaaaccaca agaaatgggc gacggaaaat gcctttgttc tacaatacca
     6781 tatgacgccc gatattacgc catacataga atatgactac cttgaccgcc agggtgttta
     6841 caacggcaga gataatttat cggaaaacag ttatcgcatt ggtgtgtcat ttaaactgta
     6901 gtagacagga gacagtcaca atgaataaaa ctataatggc gcttgctatc atgatggctt
     6961 catttgccgc aaacgcgtct gtattaccgg aaactcctgt gccatttaaa agtggtaccg
     7021 gagcaattga taacgacact gtctacattg gtttaggtag cgcaggtaca gcatggtaca
     7081 agctagatac acaggccaaa gataaaaaat ggactgcgtt agctgcattc cctggtggac
     7141 cgagagagca agcaaccact gcatttattg atggcaatct gtatgtgttt ggcggcattg
     7201 gcaaaaacag cgagggattg actcaggtat ttaatgacgt acacaaatac aaccccaaaa
     7261 ccaatagctg ggttaaattg atgtcgcacg cgccgatggg catggcgggt catgtgactt
     7321 ttgtacacaa cggcaaggct tatgttactg gcggtgttaa ccagaatatc ttcaatggct
     7381 attttgaaga tctcaacgag gctggaaaag attcaaccgc tatagataaa atcaacgccc
     7441 actattttga caaaaaagca gaagattatt tcttcaataa gtttctgttg tcttttgatc
     7501 cctcaacaca gcaatggagt tacgctggcg aatcgccctg gtacggaacg gctggtgcgg
     7561 cggttgtgaa taaaggtgat aaaacctggc ttattaatgg cgaagccaaa ccaggattgc
     7621 gaacggatgc cgtatttgaa cttgatttca ccggtaataa tttaaaatgg aataagcttg
     7681 atcccgtctc atcaccagat ggcgtcgctg gcggttttgc ggggataagc aatgattctc
     7741 ttatatttgc tggaggggcc ggattcaaag gttcacgaga aaattaccag aacggtaaga
     7801 actatgcgca tgaaggcctg aaaaaatcat atagcactga tattcatctt tggcataacg
     7861 ggaaatggga taaatcgggt gaattatcgc aaggtcgggc ctacggagta tcattgccct
     7921 ggaataatag tttattaatt attggcggtg aaactgcagg cggcaaagcg gtgacggatt
     7981 cagttttaat ttctgtgaag gataataaag tcacagtaca aaactaacgc ttcagggccc
     8041 cggtaaaggg gccctgctat caatttgtca tttgtattac gcgaaaatta tatgaacgca
     8101 ataatatcac ccgattatta ctatgttctt accgttgctg gtcagtctaa tgccatggcg
     8161 tatggcgaag gactgccatt accggacagg gaagatgcgc ctcattccag aattaaacaa
     8221 ttagcgagat ttgcgcatac gcatcccgga ggcccgccat gtcactttaa cgacattatt
     8281 ccactgactc actgcccaca cgatgttcag gatatgcagg gttatcacca tcctctggca
     8341 acgaatcatc aaacacagta cggcaccgtt ggccaggcac tgcatattgc acggaaatta
     8401 ctgcccttca ttcctgataa tgcagggatt cttatcgttc cgtgttgccg tggcggatcg
     8461 gcttttaccg cgggcagcga agggacatat tcagaacggc acggagccag ccatgatgct
     8521 tgtcgttggg gaacggatac tccgctgtac caggatttag tcagcagaac gcgagccgca
     8581 ctggcaaaaa atccgcagaa caaattcctc ggcgtatgct ggatgcaagg cgaatttgac
     8641 ttaatgacca gtgactacgc gtcacaccct caacacttta atcatatggt tgaagccttt
     8701 cgtagggatc taaaacaata ccattctcag cttaataata ttactgacgc gccgtggttt
     8761 tgcggcgata ccacctggta ctggaaagaa aatttcccgc attcgtatga agctatttat
     8821 ggcaattatc aaaataatgt tttagccaat attattttcg tcgacttcca gcaacaaggt
     8881 gaaagaggac tgacgaacgc gcctgatgaa gacccggacg atttaagcac gggatattac
     8941 ggttcagcgt accggtcacc ggagaactgg acgacggcac tgcgaagcag tcatttcagc
     9001 acggcagccc gtcgggggat tatttctgac agatttgtag aagcaatttt gcagttttgg
     9061 cgcgaaaggt gagcggggag tttattcatg ggggatgtcc ttgttcgttc tcagagtaga
     9121 caacaaaaaa caaatgagat caaggtaata tctcttcact tgcgggctcg aacagcaggc
     9181 aattgccaat ataataatct ggcacaaggg tgaaaaacgc gcaccgcata aaccgttgtt
     9241 attgcttaca gattagccgg atgccttaat agacatccgc gccttttcga ttatggctcg
     9301 gaaatctacg aacatctaca cagtttactg gaacgttttg ggccacagcg ttcgcagcac
     9361 cgtcctgata tgccgttctg gcgattataa gtcgacggat tctggcaaat agacgatgat
     9421 gatatgctta atgatgttca aagaaattag gccattatta tattattgct tgcattttct
     9481 ttcattccta aaagcacccg ccattaatcc caggcgcatt at
//
LOCUS       DAJTSI010000148         9493 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000148 DAJTSI010000000
VERSION     DAJTSI010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.148"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..190)
                     /locus_tag="OO327_003326"
     CDS             complement(<1..190)
                     /locus_tag="OO327_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HCR6019750.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLGEKA"
     gene            710..1405
                     /gene="minC"
                     /locus_tag="OO327_003327"
     CDS             710..1405
                     /gene="minC"
                     /locus_tag="OO327_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR6019751.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1429..2241
                     /gene="minD"
                     /locus_tag="OO327_003328"
     CDS             1429..2241
                     /gene="minD"
                     /locus_tag="OO327_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR6019752.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2245..2511
                     /gene="minE"
                     /locus_tag="OO327_003329"
     CDS             2245..2511
                     /gene="minE"
                     /locus_tag="OO327_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR6019753.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2883..3227)
                     /locus_tag="OO327_003330"
                     /pseudo
     CDS             complement(2883..3227)
                     /locus_tag="OO327_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3262..3381)
                     /locus_tag="OO327_003331"
     CDS             complement(3262..3381)
                     /locus_tag="OO327_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR6019754.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3342..3527)
                     /locus_tag="OO327_003332"
     CDS             complement(3342..3527)
                     /locus_tag="OO327_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019755.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3628..3801
                     /locus_tag="OO327_003333"
     CDS             3628..3801
                     /locus_tag="OO327_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019756.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3803..4147
                     /locus_tag="OO327_003334"
     CDS             3803..4147
                     /locus_tag="OO327_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR6019757.1"
                     /translation="MKKKILAFGLISALFCSTPAIADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVSAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4151..4486
                     /locus_tag="OO327_003335"
     CDS             4151..4486
                     /locus_tag="OO327_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR6019758.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4543..6864)
                     /locus_tag="OO327_003336"
     CDS             complement(4543..6864)
                     /locus_tag="OO327_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6019759.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKDETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6981..7190)
                     /locus_tag="OO327_003337"
     CDS             complement(6981..7190)
                     /locus_tag="OO327_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019760.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7941..9464)
                     /locus_tag="OO327_003338"
     CDS             complement(7941..9464)
                     /locus_tag="OO327_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6019761.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTADVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIASAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT         2659 a         2206 c         2079 g         2549 t
ORIGIN      
        1 cggctttttc gccgagatat ttccttgtta actctgggga aatgccatct gagttcacac
       61 aaatatacgg gtcgcaaaca acgccgcacg tcggagattg aagtccgcca gcgtcatgtc
      121 cagacgccat tgatgagaac atcatcccca cacctgacaa tccgatataa ataatacgca
      181 tcatattcat agcacatcct gataatccaa ccgtacaaca cttatgctca tttcgtcatc
      241 aatccgtaat caaaaccgct gacctggacg agttacgatg acctgacaga agtatcagac
      301 gtcttctgcg gtttctatga tagagcaatt ctgtggcgga tattaagata acgggtatct
      361 gttctttaat gatgctgcaa aaaatggaaa gttaagaaag cctggcgtgg tgaaaaaata
      421 cacttgcctc aatactggat atttaaacag gtctggagat gctgtcacga ttagggtgcg
      481 gaaaaaggaa gcgtaaataa gaagaatcaa aagtacgacg gcaatgggtt gattgacaag
      541 ggtatttttt aagctatgaa tcagcgccat ttatcacaga atagactttt actctgaata
      601 aatgggaggg tgacttgcct caatataatc cagactataa catgccttat agtcttcgga
      661 acatcatcgc gcgctggcga tgattaatag ctaattgagt aaggccagga tgtcaaacac
      721 tccaatcgag cttaaaggca gtagcttcac tttatctgtg gttcatctgc atgaggcaga
      781 acctaaggtt atccatcagg cgctgaaaga caaaatcgct caggccccca catttttaaa
      841 acatgccccc gttgtactca acgtcagtgc actggaagac ccggtaaact ggtcagcgat
      901 gcataaggcg gtttcggcaa ccggtttgcg ggttattggc gtaagtggct gcaaagatgc
      961 gcaacttaaa gccgaaattg aaaaaatggg gctgcctatc ctgacggaag gaaaagaaaa
     1021 agcgccacgt ccagctcccg caccgcaggc tccagcgcaa aatacaatgc cggtcacaaa
     1081 aacgcgttta atagataccc cggtgcgttc cggtcagcgt atttatgctc cacaatgtga
     1141 tctgattgtt acaagccacg ttagcgctgg ggccgaattg attgccgatg ggaacattca
     1201 tgtctatggc atgatgcgcg gtcgtgcgct ggcaggggca agtggtgacc gggaaacgca
     1261 aatattttgt acgaacctga tggcggaatt ggtgtccatc gcaggtgaat actggctgag
     1321 tgatcaaatc ccagcagaat tttatggcaa agcggcgcga ctgcagttag tcgaaaacgc
     1381 tttgaccgtt caaccgttaa attgatccct ttttaacaag gaatttctat ggcacgcatt
     1441 attgttgtta cttcgggtaa agggggtgtt ggtaagacaa cctccagcgc ggccatcgcc
     1501 actggtttgg cccagaaggg aaagaaaact gtcgtgatag attttgatat cggcctgcgt
     1561 aatctcgacc tgattatggg ttgcgaacgc cgggtcgttt acgatttcgt caacgtcatt
     1621 cagggcgatg caacgctaaa tcaggcgtta attaaagata agcgtactga aaatctctat
     1681 attcttccgg catcgcaaac acgcgataaa gatgccctca cccgtgaagg ggtcgccaaa
     1741 gttcttgatg atctgaaagc gatggatttt gaatttatcg tttgtgactc cccggcaggg
     1801 attgaaaccg gtgcgttaat ggcactctat tttgcagacg aagccattat taccaccaac
     1861 ccggaagtct cctcagtacg cgactctgac cgtattttag gcattctggc gtcaaaatca
     1921 cgccgcgcag aaaatggcga agagcctatt aaagagcacc tgctgttaac gcgctataac
     1981 ccaggccgcg taagcagagg tgacatgctg agcatggaag atgtgctgga gatcctgcgc
     2041 atcaaactcg tcggcgtgat cccagaggat caatcagtat tgcgcgcctc taaccagggt
     2101 gaaccggtca ttctcgacat taacgccgat gcgggtaaag cctacgcaga taccgtagaa
     2161 cgtctgttgg gagaagaacg tcctttccgc ttcattgaag aagagaagaa aggcttcctc
     2221 aaacgcttgt tcggaggata agttatggca ttactcgatt tctttctctc gcggaagaag
     2281 aacacagcca acattgcaaa agaacggctg cagattattg ttgctgaacg ccgtcgcagc
     2341 gatgcagaac cgcattatct gccgcagttg cgtaaagata ttcttgaggt catttgtaaa
     2401 tacgtacaaa ttgatcctga gatggtaacc gtacagcttg agcaaaaaga tggcgatatt
     2461 tctattcttg agctgaacgt gaccttaccg gaagcagaag agctgaaata agcccgctgt
     2521 aaaaacgcat ttatcttcaa ggcagagtca tttctgcctt gagtttttca tccctctcat
     2581 ccacgttgtg gtaaagcggc gagtattctt gctgatactc ctcattgcta tttccacctc
     2641 cccctttaca ccttaaggct gttcccacgc acagaacaaa atgtttatgc ctggttgagt
     2701 aaataaccat gttattagtg tgtatatact gggagatgat aaccccgcaa tttcattatt
     2761 cattgttctc ccataggatg aggtttcccg ccgtaactgg cgggcttttt ttgccccaga
     2821 attttctctt tcaacatcct gtaaacgaaa actgcgccga agcgcagttt aatcgttgat
     2881 ggtcagaacg accagttcac accagccacc gcgttccacg gggactccac accggcacca
     2941 tggctatacc ccaccccgaa atgcccgctt aacgtactgc tgaatgaggc tttaatacct
     3001 gcctggtata ttccacgtct gcccgacaaa tcattgacaa aattaccgtc actattcact
     3061 ttcacccgat tatcatcgac aaattctttg cgcacagccg ccttcaggca gggttcaatt
     3121 tccataccgt tccccagacg catgttgtaa ctcagcgttg cacccagttc acgatatata
     3181 ctgcgggtat cgactgattt cgattccatg ccattggata aatgatagta gcgtctaagt
     3241 gacctaagct ctgctgcaac atcagcccgc aggcaccaga ccaggggatt catcctgaag
     3301 agacagcgca ggtgtattgt gttcaccgct caccaaagac atcatgatga aatgatgata
     3361 tcccgcataa gaaagaggca ttttttacac gcagtgcgct gaagtgtagt tggataaaaa
     3421 atcactcttt tcagataata ctgtcgtacg cttatcttta gacaatgagg ccgtcggaaa
     3481 aataaaatga ctaatgtaat cgttgctgat gaatatcttc tgctcacata aaaatcacac
     3541 aataatattg ataaatcaga ttatcttacc tttacaacat tgcgttaaca gtcaatgaca
     3601 cttcttgaat aaaaatattg tcaatcaatg agttattcct catttaagat tattctcatt
     3661 cacgtaaaaa acataattcc tatcatcaca gccacgctca ttttgaatta tctgaataat
     3721 agcgagcgtt ctttagttaa gcagatctta attgaagacg aaattattgt gtgtgcaact
     3781 tacttaatac cggacatcta agttgaagaa aaaaatactt gccttcgggc ttatctcagc
     3841 attgttctgc tccacgccag cgatagcgga tatgaaccgc accacgaaag gtgcattgct
     3901 aggcgcaggc gttggtttac ttactggcaa tggtgttaat ggcgtactga aaggtgcagc
     3961 tgttggcgct ggtgttagtg cagtaacaga aaaaggccgc gacggtaaaa atgcgcgtaa
     4021 aggtgcaaaa gtgggtgccg ccgttggtgc tgtcactggc gttctgaccg gcaatggcct
     4081 cgaaggggcg attaaaggtg ccgtgattgg cggtacgggt ggtgcaattc tgggtaaaat
     4141 gaaataagga atgatgatga aaaaatttgc tttactggcg ggcctgtttg ttttcgcacc
     4201 aatgacctgg gcgcaggact acaatattaa aaacggtttg ccgtcagaaa cctatattac
     4261 ttgcgcagaa gccaacgaaa tggcaaaaac tgacagcgca caggttgcag aaattgttgc
     4321 ggtaatgggt aatgccagcg ttgccagccg tgatttaaaa attgagcaat ctccggaact
     4381 gagcgccaaa gtggtagaaa aactgaatca ggtttgcgct aaagacccgc aaatgctgtt
     4441 gatcacggca attgatgaca ccatgcgtgc tatcggcaaa aaataatatt tcatatgtac
     4501 cgcgtcgatg aatacgacgc ggttttttag ctaacggcgg gatcagaact tatatttcag
     4561 gcccaccatc actgcggtgt cattgtagcc attatcaccc agctgcacgc ccacattccc
     4621 ccagacgctg gccgctggat tcaggttgcc atttacgcca agtttaattt cccccaaatt
     4681 actcacccca tcttgttcga ctttcacacc gttcattgag acagcaaaat ctttactgtt
     4741 atggatccag ttcatttcaa caaaaggttc aaagtcgtca ccgtgcggac cgctgttttt
     4801 ctcctgagta cgaataaagg tgcggaagcc gagtcgggtc tggatgttgt tatttgcgtc
     4861 attctcaata cgtgagccat tggcttccgt gtgcgcatta tgatcgacgc ccatccagat
     4921 agcctgtgcc tgtggctgga agatccacgt atatttcgcc gcttccagac caaaggattt
     4981 attcagggca aaaccgtaac ccgcttccag cgagacggtt gcacctttcg cgctatagga
     5041 ttcataggct aattcatccc ctttcacgga gtttttaaac cagctatatt gcgcccagga
     5101 gtcaatgtat gcgccttttt tactgatgtc atcggcaaac caagtggcat acagccctgc
     5161 gctatagcct ctgacactcc ctttcgaacg ataatccgac acgctggaat gagttaagtt
     5221 gtagtcgcgg gcataaccag ccatcactcc caaacgccaa ctgtcactat cggtaaaacc
     5281 accggttaac agatcgcccc ctaactgcga gacgtagcga tgcgaggtcg ttctcaactg
     5341 tccgttgctg tcacgccagg cattatgccc gccaatttga cgtagccaaa gtcggcttga
     5401 acgctcctgt tcagttacag gatcgatgta gcgcgtttca cccgcacggt cgttcagacg
     5461 cataacaaaa agagtgttag cggctgccag gttggcgacg taacttccag cttcggggcg
     5521 caggacgggg gtaggcgcaa cagtaggcgc tgaacttcct ccggagtttg tttcaccgcc
     5581 gctcgttgcc tggaagttag tcagatacca gtttttattg gtcgcttcgg tattacctcg
     5641 ggttaatgaa tattcgtagg caccggcgaa aatcctcgaa tccttaataa attccccatt
     5701 tgattccccc tcaacgctga taatctctat cccatttaac gtgtaagctc cgctgcctcc
     5761 tgcattggta actttaactc gggttgttcc agaggtattt ccattaataa cgactttatc
     5821 cgtagctgaa ttatcatcat ccaattcagt tctgagatgc aatacgccat tattaccaac
     5881 gtaattttca gttattgtta atcttgttgg ttcgacatcc cgtccatctg cccgggaaat
     5941 atagaccgta ctattatcaa ccgtgaggtt gctcaccgtt gagtcagctt taatatccca
     6001 ggtactatta tctgaaagcg acagataggt atgagtgtta tcatctgttg aaatattagc
     6061 tgaacccgtt atttttgaat tagttgcatc gacggtcaaa tgacctttaa tattattgat
     6121 aaaaacatct gcagccgatg atacctcact atccgtaatg gtcacgttac tcgtctcatc
     6181 tttatggtag aggatttcat ttgctgaccg tagtgagcta ccagataaaa tatctaccgt
     6241 tgcttcctga ccatttatgg aagactccag cacaattcca gacccctgat gcgcgactgc
     6301 agatgtattt ttcagcgtca ccgtgccgcc attataaatt ttagcaatcg cggcgtcagg
     6361 acccgttgta taaatttctc cctggttaaa tgaaactgtc ccctggcggg cagcgatggc
     6421 atgagcattg tccccatgcg tggtgatatc aacatcggtt aatgtaacgc tggcattggt
     6481 ataatcaagc gtgtcaccag ccttgacgac agtgccatta gcgtacgcgg cataactttc
     6541 tgttccgtgg gttgtgatct ccccttttgc catatccagt tttgctatgg cattattttc
     6601 attacttcct gtatcggcag tactttcttc atcaagagaa gatacacacg cacacgcgct
     6661 tccgggagat gcacacgcaa gaacgccgac tgcgtgatta cccgcagtaa caataaatgt
     6721 atcatctccg tcagaaaaag agggggtaac cgaaccgtca gagttgactc ttattatggt
     6781 gtcagtattc aggtttccac tcgtttgact tagcaaacca attgagtaat cccccgttgt
     6841 gacaatcgtg tttttaccct gcaaaataac agcaccgcca aattcactta cgattgcgct
     6901 agctgttaat gctgggctta gaggactatt gtcagaagga tataaaccgc ccgttgttac
     6961 aatgacgcca ttgttaatgg ttaacttact accctctcct gcaacggata cccgtaactt
     7021 ccattacccg ttgttataac tagcgcatta tcaatatctt ttacttttcc atcttttatt
     7081 gccacagcat gtttatattc atcgtgctga ataaactgtt cagcatatgc attacccacc
     7141 gccagagcaa taagtcccaa actaaacccg gggagttttt tcagtttcat agattaacta
     7201 tccattcatt ggattaacaa acattcctgg tcaaataacc tatgagctca gattaccaaa
     7261 acggagggta aagacaaaaa caaaagcaca aaaaatttaa atagataaac agcgacaata
     7321 tataaatgat aaataagtcg gcattatcat ttttcacctt attttcatga cattgatcac
     7381 tttggggtga ctattttgtt taacaaataa ctcttcgaca tcaaaattat taatagttgc
     7441 catggtaatt atttcggtga tatataaaaa caactgtgat aacaccaagg ttgacaacta
     7501 tttgcgcaat aattcctttc cttcaatgat ccaggcgttc actgccagca acccattcag
     7561 ccgcatactg acagtgagaa actgaaagat tattgctgat ccctgtggct aacgagtctt
     7621 tctcttaaat cgattaacca gattgatcgc cagccaaaca agggcattaa gcaaatttat
     7681 cgtcacaagc acttctccca gcccggtact gggttttgca ataaggatga ccaggagggt
     7741 gatcagcata aatgcaatgg aaaagatgat atagagagtg aaataatcca gattattact
     7801 attattcatg ctactaatgg caatcctgtc aaaactttca aacaattata gcaaacaaaa
     7861 taatgaacaa tagtaacaag aggcaacgag ctaattagct gtctataatt acaccgcact
     7921 atggccttac tgatcaattt tcactcaacc acaaccttca ccttcggttt agaaaggata
     7981 attttgacca ggtcgatgta tgtaacaggt ttatagaaat aataaccctg ctgaaatgtg
     8041 atattatttt ggttcagata gccaagttgt tctttcgttt cgacaccttc agcgacgata
     8101 ctgatcgaaa gtttacgcgc cagatccaat acacaatcca gaattcgggt tgattctggg
     8161 tctgcgttta cgcggccaac gaaactgtga tcaattttga tataatcaat atgcaggtca
     8221 tgaagataag aaagccctga gtagccagta ccgaaatcat ccagcgcgat gacaaaacca
     8281 ttttcatgca gaatgttcaa ccgctgaacc agactttcat caacattcaa tggctcacgc
     8341 tcggtgactt ccagaacaag gtttaaatcg cggcgggtaa aactgtcacg gaaatttaaa
     8401 cactcgtcga caaacgtcgg cgaaataata tgcgaagcgc taaaattaat tccaatatga
     8461 aaaccttccg gcagtttact cgcgatagcg ttcatctgtc tggcaacctg attcatcagg
     8521 ctttgtgtaa gcggaacgat taatccggat ttttcagcaa gtggaataaa tgccgcgggt
     8581 gaaatatatc caccgtgagg ttgtttccag cgggctaaca cctcaactcc ccgcaatgtc
     8641 ccttcccgac catttaccac aggttggtaa aaagggacga tctccccatt atttattgcc
     8701 cgtcgcagga tctcttcagg cgttgcgctt ttattgaaat atctatcaag caggaaggca
     8761 gcggcgctgg cgattaacag aataaatatt aaaatcccaa agcctttatc gataagtctg
     8821 tagaagctaa agagtggcgg cgggttaaag ataatagtga aatgatattt tgtggagtga
     8881 accttctcgg tatagggata atgtccatta agcgtcatca catcagccgt tggtccaata
     8941 atgtcatccg ccacgcgtaa tacatacctt acccctttca agggtacatt aagcgccccg
     9001 cgaatatgtt gatcacttat cgtaaccaaa atgcgcgtat ctgcaaattg gctctgatag
     9061 agcaggatag gtaatctatt aacggtgtcg attgccggag ccaacagtaa attactgtcc
     9121 gggacaacag gaatacgaga cagcaggacc cgattcccag gcagggatgt acaccagact
     9181 attccctgtt tgagaatgat gattgtgcgc aggtgaggtt tcagagctgc ttcagtgcca
     9241 agctgatatt gctcctcgag gtcgcattcc ttcccggcaa tgttaaccgc tgtttgagtc
     9301 gcctgtgacg cttcttcaag tatatgattg atattgttgg cggcatatct tgctcccgcc
     9361 aggtactctg cttttgcgga ataccagagc tggatgttca aaatagcgac accggtaata
     9421 aacaagcata tcgcaacgag gatgggtatg agtgtattgc gcatttgctt gtcccggcca
     9481 aagggtttaa tac
//
LOCUS       DAJTSI010000149         9440 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000149 DAJTSI010000000
VERSION     DAJTSI010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.149"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            39..1154
                     /locus_tag="OO327_003339"
     CDS             39..1154
                     /locus_tag="OO327_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019762.1"
                     /translation="MFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVGPDILAL
                     PVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFD
                     LRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRSVALAKT
                     VAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISVRNKYSP
                     AFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAKDRTISI
                     VLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKDLFDIRVLHVSNK
                     FNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            1833..2051
                     /locus_tag="OO327_003340"
     CDS             1833..2051
                     /locus_tag="OO327_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR6019763.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2269..2511
                     /locus_tag="OO327_003341"
     CDS             2269..2511
                     /locus_tag="OO327_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019764.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(2841..3770)
                     /gene="fdhE"
                     /locus_tag="OO327_003342"
     CDS             complement(2841..3770)
                     /gene="fdhE"
                     /locus_tag="OO327_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR6019765.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(3767..4402)
                     /gene="fdoI"
                     /locus_tag="OO327_003343"
     CDS             complement(3767..4402)
                     /gene="fdoI"
                     /locus_tag="OO327_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6019766.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4399..5301)
                     /gene="fdoH"
                     /locus_tag="OO327_003344"
     CDS             complement(4399..5301)
                     /gene="fdoH"
                     /locus_tag="OO327_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR6019767.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5314..8364)
                     /gene="fdnG"
                     /locus_tag="OO327_003345"
     CDS             complement(5314..8364)
                     /gene="fdnG"
                     /locus_tag="OO327_003345"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7777..7779),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6019768.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            8558..9391
                     /gene="fdhD"
                     /locus_tag="OO327_003346"
     CDS             8558..9391
                     /gene="fdhD"
                     /locus_tag="OO327_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR6019769.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2311 a         2315 c         2446 g         2368 t
ORIGIN      
        1 ttgaggatat gtgtgaaaga atgaaaggtg gcatctctat gtttaaccac accacaaaaa
       61 cagctgataa tatagagcat aatttgcagc taagttttct cgtgggttca cgtccgtcga
      121 tcaccaataa atactcagca aaagatgtag gtccagatat acttgcattg cccgtcaacg
      181 tatatcatgc ggattttaaa attaaaatca ataatgcagt ggagaaggct tcatcaattg
      241 tgttatgtgc tgtgcaaaaa aatctccatg cagcaaggaa ggttcttagc gggataatag
      301 gaaatgtgcc aaaaaataat aaaccaaaaa caattatttt tgatttgcgt tttcttagtg
      361 caattaagca gaaagttttt ttgggcagtg aaaaaccgtt gtttgtaaag cataccgcaa
      421 tgatgaggga ggcatgtaag gaattaccgc agtcagtgga atatgttcct ttagcctgtg
      481 aagcgcatag ccatcgcagc gtcgcccttg ccaaaaccgt ggctgcgccc atcatggcga
      541 caggtgtggg cttggcatta atgtatgcaa catcatcagg accgtattat gcgcaaagtc
      601 tttctggttc gccggatatc gagatgataa aaaatcgcat ggctgaattg tttaaccagt
      661 cggatatttc tgtgcgcaac aaatatagcc cggcttttga taaatggaat gaactggtag
      721 ataaattaaa tcacgaaaga aatacagttc cgtttgcgtg tttgaccacc attttagcga
      781 cggcgataaa tgaaactctt gagggggata cgaatgttgc cgttgtgatg gggtgtaaat
      841 ctgcgaaaga tcgcacgata tccattgtcc ttggcaactc gatgttgcaa acactccttg
      901 aaaaacgcct cgccgatagt ggagaaatag aaaatctttt tgatcaacag ggatatttta
      961 gttgcgattc attaacaccc gaagaattaa tgatgttaaa agaccttttt gatattcgag
     1021 ttcttcatgt ctctaacaaa ttcaatgttg gattgcaagg taatatcaac actgatgttt
     1081 tacaagattc gttttttaaa aatgtcgatt tcattcatga atataactcg tataccgtag
     1141 ggatgggagt ctgacgagta aaactcatta ggattatcag aattcggaca atgcattgca
     1201 aaaatgacat acaaagagag atgtgtgcag gtacttagcc gcgaggggca tggcgaagcc
     1261 atacagaaag taccagtatt cagaactcca gtgaaagcgg catagtggcc gcctgctcag
     1321 gagttttgct atgacccgta atcgtgctga acgtcgccac cctatgatgc gattaaaaaa
     1381 actcatcgtc gcgctgatga ttgacgtcaa tgctatcgga tgatgccggg ggtgttccgg
     1441 aaggaattcc ttgtttcccg aactgttacc aacaaaatta taacgatgag caagtaaaga
     1501 ccggttggtg gcagtactga ccggtctgtg atgaaagatt aatttcacct taaatcctgg
     1561 tgctgttagg atgctgacgt ttttcggcta caccattcag attatgtaaa taaatatccc
     1621 tgaatataat atattcatag cacccataac tcatcctggg cgataactaa accatttttg
     1681 agtaataaat gaaacaactg caacgctctt attatttctg gaaaacgcct cgcaaaaaca
     1741 aaatgacatg caaaaatccg cattttttta aatgcttttc cggcgtaatt ttcgcacata
     1801 cattaatgag taatatatgt aaggtggtgg taatggcgat gaatacggtt tttcttcatt
     1861 tatcagaaga ggcaattaaa cggctgaaca agcttcgggg ctggcgtaag gtttcacgat
     1921 ctgcaatttt acgcgaagcg gttgagcaat atctggagcg gcagcaattt ccggtgcgta
     1981 aagcaaaggg aggcaggcaa aagggggagg tagttggtgt tgatgatcag tgtaaggagc
     2041 ataaggaatg aaaaaaattt ttcattcttg aatataaaaa acagatgccc ttattctggt
     2101 attaatacaa ggctgtttta cttgaactta taataactgc aactgttaca tcgtatctgg
     2161 aaaacgcctc gcaaaaataa aaaatgatgc gtaaatgagc tttctttaaa gattcggcaa
     2221 gggtactttt ctctccatag attcaaaaat gatattggcg agatatttat gaactctctg
     2281 gcaggtatag atatgggcag aattttactc gatttatcga atgaggtgat taagcaactt
     2341 gatgatctcg aggtgcagcg taatcttcct cgtgcggacc tattaaggga agcggtagat
     2401 caatacctga taaatcaatc gcaaacagca agaaccagtg ttcctggcat ctggcaaggg
     2461 tgtgaggaag atggtgtcga atatcagcgt aagctgcgcg aggaatggta atgggaaaaa
     2521 atgatgtcaa tcagattgct gacaacgtgc gcgttgttca tgccggatgc ggcgtgaacg
     2581 ccttatccgg cctacaaagt catgcaaatt caatatattg aagtttattg gtaggcctga
     2641 taagcgcagc gcatcaggca atcatacgct tgtcatcagt gacataccca taaccttatc
     2701 gatgtgttgg agggaagaac gaggcaaaca gacaattgcg gcatgatctt gaatgggtaa
     2761 cgcggaatac gagagatggt gtcgtcacac cctatgagct gtaatccggg cgacctgcgc
     2821 cacccggaaa acgtcagaga ttactctcct tcacccggaa acaggaacgg gttaatggaa
     2881 ctacgggcgt agccttcttg ctccattcgc gcgtccagta ccagagaggc gaggtcatct
     2941 gccacggctt caactttcgg ttctttttcc tgataaagaa tcttcaggta agtgccgcag
     3001 tcatcgcagc tttcggcttt aatcgcggcc tgttcgtcat ccagcgacca gtaatgcagt
     3061 ttgccgctct gctcgcagtt gctgcatttt acgcgcacta cgtgccattc ggtttcacac
     3121 aggttgcagt gcaggtaacg cagaccctga gtggtgccaa tttgcaccat gctggacacc
     3181 ggcatagagc cacataccgg gcaatattga cgttgttcgc cgtattcagc gcgggctttg
     3241 ccggggatca gattggccat ctgcgcccag tagagcgaca gcgcagccca gataaacggc
     3301 gctttatcgc tgctgacgga cgagaaatca gaggcaaaca gcgcgctggc catatcttcc
     3361 agctcctgag tcgatgcctt ctccagattc tcaatcactg ccagcgccgg gccgctcatt
     3421 tcaggtttca gctcagcaat tagcgccatc agcagctttt gccagtgctt atcacgcggc
     3481 agaacgtgaa tatccagcgg gggcttgccc tgtgcgctgg cttctttaat gcgcgcggtc
     3541 agatccatct ccagcggatg gtcgtacagc accactttct gggcgtgggc gataagcgca
     3601 gcaaagcgca ggtaatcacc cagcggatta ttttctgcca gctcgcgcag acgctcggcg
     3661 cggcggttgt ataaattctt gagccgaggg aacaataacg gcggaatcat atccgccgta
     3721 cgtttctcgc tcgaacccag ctcatcttgc gggattatgc gaatactcat tcagctttct
     3781 tttccgttgt cttgcggacc tcacggtacc agcgcgggtg atgtttcttc gcccaggcgc
     3841 tggtaaccca tccttccacc atggcggtaa tcgtgccttt cacccaaagg gcggcgtaga
     3901 tatgcaccat gataaccaca attaacgcca ctgcggcaaa tgaatgcagc attaacgcga
     3961 atcggatcac cgggattgag aaagcaggcg caaaataagg acgccagata atcacgccgc
     4021 tcaccagcag cagaaccagg aaaataatcg ccgcccagaa aacgcatttc tgaccgaagt
     4081 tataacgccc ggtgtcacct acttcctcgt tgacgacgat cttacgaata ttcttcgccc
     4141 aaaagatatc atcccgattg attaggtttt gatgccagta acggaaaaac atgatgatga
     4201 acgaggcaaa cataaccacg ccgacaaacg ggtgcagaat tcgcgccagc tgcggtgtgc
     4261 ccatgatttg catcaaccag ttgaaggacg ggaacaaaaa gcccagcccg ctcaccgccg
     4321 ccaggatgaa gcagaaggcg gtgatccagt ggttgatacg ttccggcgcg gtgtagcgca
     4381 cgatggtgtc acgtcgtttc atttgcgctc ctcgtctttc tcttcgtgca gattattctc
     4441 ttcctcatcc gcacggttcg gaccgacacc cacgtagtgg aagatactgg ccgcaaaggt
     4501 agccgcaaag ccaacagccg cgagcggttt ccagatgcct ttccagaatt tcacggtttc
     4561 gctgatttcc gggttctccg gcaagccatg atacagattt ggcttgtcag catggtgcag
     4621 cacgtacatg acgtgtgtac caccgacgcc tgccggatcg tacagacccg cattgtcgta
     4681 accgcgggtt ttcagctcag caacgcgctc gctcgccagc gttttcatcg actctttcgt
     4741 gccgaagtga atcgcgcccg ttgggcaggt cttcacgcag gctggttctt gcccaaccac
     4801 cacgcggtca acgcacagcg tacatttgta gacgcggttg tcttccgggt tgaggcgcgg
     4861 aatgtcgaac ggacagcccg caatgcaata accgcagcca atgcactgct cggactggaa
     4921 gtcgacgata ccgttggcat actgaataat tgcaccttcc gccgggcacg ctttcaggca
     4981 gcctggatcg gaacagtgca tacagccgtc tttgcggatc agccattcca gtttgtcgtt
     5041 ctgctccact tccgagaagc gcattaccgt ccacgatttg gcgcttaaat cattggggtt
     5101 gtcgtacacc ccaatgttat tgccgacggt atcgcgaatg tcgttccact ctgaacacgc
     5161 cacctgacag gctttacagc cgatacaggt ggtaacgtcg atgagtttcg ccacttcttc
     5221 ctggaagtcc cgcgcctgag gcgcgggggt cagaccgtta gtcgcggaac gacgaatgat
     5281 atcttgagat tgataagcca tatgtcgtct ccgttacacc ttttccacat tcacaaggaa
     5341 ggacttaaac tccggcgtct gcgtgttcgc atcaccaacg aatggcgtta aagtattggc
     5401 aataaagcct tttttagcta cgccttcata gccccagtga atcggaatac cgatggtatc
     5461 gatatctttg ccgttcgctt tcagcgtgcg aatacgtttg gtcaccaccg ctttggcttt
     5521 gatatagcca cggttggagg agactttcac ggtatcgccc tgggcaatgc caagtttatt
     5581 cgccagcgac tccccgattt ccacaaactg ctctggctgc aaaatcgcgt tcaacagcgc
     5641 gtgtttggtc cagtagtgga agtgctcggt cagacgatag gtggttccga catacgggaa
     5701 cttatcggct ttacccaatg cttcggcgtc gtctttaaag atacgtgcag ctgggttcga
     5761 gataacgttt ggatgcagcg ggttagttcc cagcggcgtt tcaaacggct cgtagtgttc
     5821 cgggaacgga ccttccgcca tcttatcgag ggcaaacaga cgccccatgc cttcctgctg
     5881 catgataaac ggcccgacgc cgctacccgg aggcgctgcg ctgtagtccg gaatatccca
     5941 gccggtccac ttagtgccgt cccatttcag caactgacgc ttcggatccc acgggttacc
     6001 ctgcggatct gcggaggcgc ggttatacag aatgcggcgg ttaagcggcc atgcccatgc
     6061 ccagcccagc gtgttaccga ggccagacgg atcggcgtta tcacgacgcg ccatctggtt
     6121 gccttccggc gtccagctac cggcgaaaat ccagcagcca caggaggttg taccgtcatc
     6181 gcgcaattgg gcgaacgagc taagttgttg gcctttcttg acgataaccg caccggttgc
     6241 cggatcggta atatcggcca gcgccttacc gttgctctcc atcgctactt cttccgactt
     6301 cggctcatgc ggaatggcgt agttccaggt catgttcagc acctgatccg ggttcgcgcc
     6361 accctgttcg gcatacatct tgcgcaagcg caggaagata ccggagagga tctcaccgtc
     6421 agtcagcgca atccccgggg cgtccgcacc tttccagtgc cactgcaacc agcgacctga
     6481 gttaacgatt gaaccgttct cttctgcgaa gcaggtcgat ggcagacgga acacttcggt
     6541 ctggatcttc gacgagtcaa cttcgttcag ctcaccgtgg ttctgccaga agttagaggt
     6601 ttcagtgttc agtgggtcga tggtgacgag gaacttcagt ttcgacagac aaccgatcac
     6661 tttgtttttg ttcgggaatg aggcaacagg gttaaagccc tggcagatat agccattgac
     6721 cttgccctct ttcatcatct cgaagtattg caggacgtcg tagcctttat cccacttcgg
     6781 caaccagtca aagccccagc tattttccgc cgtcgcttta tcaccaaaga aggccttcat
     6841 catagagacg aagaatttcg ggtagttgcc ccagtagttt acctggcctt ccagcagcgg
     6901 ttttggcgtg ttggcggtaa ggtaggtttg cagatcggtc tgcttctcgc ttggcagcgt
     6961 catgtaacct ggcagactct gcgacagtag ccccaggtcc gtcagcccct gaatattgga
     7021 gtgaccgcgc agggcgttaa cgccgccgcc ggccatcccc atattgccga gcagcagctg
     7081 gatcatcgcc atcgtacgaa tgttttgcgc accaacggag tgttgcgtcc agccgagggc
     7141 atacaggaac gaggcagttt tatcgtgagc actggtttct gcgatgtatt cgcagacttt
     7201 caggaacgcg tcttttggcg taccacagat gttttcaacc acatctggcg tgtagcggga
     7261 aacgtgctgt ttcagcaagt tccacacgca gcgcggatgt tgcagcgtgg tatcgcgttt
     7321 ggcgaagccg ttttcgtcca gttcataagt ccaggaggat ttatcgtact tgcgtttttc
     7381 cgcgtcgtag ccggtgaaca ggccatcttc aaagccgtaa tcctcacgca cgatcaggct
     7441 ggcgttggta taggcttcgg tgtattcgtg gttgaatttt tcattgttca gcaggtacag
     7501 caatacgcct gacaggaaag caatgtcagt accggaacga ataggggcat agtagtcagc
     7561 taccgccgcc gtacgcgtaa agcgaggatc gatcacaatc agcttcgcgc cgttgtgaat
     7621 tttggcttcc atcgcccagc ggaacccgac cgggtgagct tcagcggcgt taccgcccat
     7681 caccacgacg aggttggcgt tcttgatgtc gacccagtgg ttggtcatcg caccgcgacc
     7741 aaatgttgga gcaagacttg ctaccgttgg tccgtgtcag acacgcgcct ggttgtcgac
     7801 cgcgagcata cccagcgcgc gggagaattt ttgcgttaaa tagccggttt cgttactcga
     7861 cgcggaagca cacagcatcc cggtggagag ccagcggtta acagtcacgc cttcggcgtt
     7921 ttgcgcaatg tagttagcat cgcggtcttc tttcatcagt ttggcgatgc gatcaaacgc
     7981 ctcttcccaa ctgatttgct gccatttatc agaacctggc gcacggtatt ccggaaactt
     8041 cagacggctt tcggagtgga tgaaatccac caggccagcg cctttcggac aaagtgcacc
     8101 gcggttgacc gggtgatccg gatcgccttc gatatggaag atagatgctt tggcgttttt
     8161 tgctccgtca ccgaggctgt acatcaacag cccacagcct acggaacaat aggtgcaggt
     8221 attacgggtt tcgcgggtgc gcagcagttt atactgccgg gtttccgcga gtgctacgct
     8281 gggtgcaaaa cccagtgccg ctgccgtggt gcctgccata ccgccagcgc agatcttaaa
     8341 gaactgcctt ctgctgacct gcatggattg ctccttgttt cgacattgtc acgtcccatt
     8401 tacattcgct tgctgcgtgt gcagggagtg ggagttattt ttctttgcgg aaggggccgc
     8461 aaaggtccag aattggctca atttccctcc atctaggaag ggtttgtaac agaataccat
     8521 aatgttggtg tgtatgttct tatctggtta agagaaagtg aaaaaaacac agcgaaaaga
     8581 aatcgaaaat gtgacaaata tcacaggtgt tcgtcaaatt gagttatggc ggcgtgatga
     8641 tttacaacac ccacggttag atgaggtcgc ggaagaagtt cccgttgcgc tggtctacaa
     8701 cggcatttcg catgtggtga tgatggcgtc gcccaaagac cttgagtact ttgcgctcgg
     8761 tttttcgctt tccgagggga taatagaaag tccgcgcgat atcttcggca tggatgtcgt
     8821 tccttcctgt aatggtcttg aagtacaaat tgagctttcc agccgccgct ttatggggtt
     8881 gaaggagcgc cgccgggcgc tggcgggacg tacgggctgt ggcgtatgcg gcgtggagca
     8941 acttaatgac atcggaaaac cggtgcagcc gctaccgttc acccagacgt ttgatctcaa
     9001 caaactggat gatgcattac gtcatctcaa cgattttcag ccagtggggc gactgactgg
     9061 ttgtactcac gccgctgcct ggatgttgcc atctggcgaa ctggtcggcg ggcatgaaga
     9121 cgtgggtcgc catgtggcgc tggacaaact gttaggccgc cggtcacaag aaggggaaag
     9181 ctggcagcaa ggtgcggtac tggtttccag tcgtgccagt tatgaaatgg tgcaaaagtc
     9241 ggcgatgtgc ggcgtagaga ttttgtttgc ggtgtctgcc gcgaccacgc ttgctgtaga
     9301 agtggctgag cgctgtaatc tgacactggt aggtttttgt aaaccgggtc gggcaacggt
     9361 ttatacccat ccgcagcgtt tgagcaatta attaaaataa gtcctgcgat atatattaaa
     9421 ttgcaggact tattcatttc
//
LOCUS       DAJTSI010000150         9242 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000150 DAJTSI010000000
VERSION     DAJTSI010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.150"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OO327_003347"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OO327_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR6019770.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISSGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLMTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OO327_003348"
     CDS             1828..3147
                     /locus_tag="OO327_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR6019771.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3445..3486
                     /locus_tag="OO327_003349"
     CDS             3445..3486
                     /locus_tag="OO327_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019772.1"
                     /translation="MVQLVDLARCVSF"
     gene            3524..4669
                     /gene="dosC"
                     /locus_tag="OO327_003350"
     CDS             3524..4669
                     /gene="dosC"
                     /locus_tag="OO327_003350"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCR6019773.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4594..5211
                     /locus_tag="OO327_003351"
                     /pseudo
     CDS             4594..5211
                     /locus_tag="OO327_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5469..5801
                     /gene="ddpX"
                     /locus_tag="OO327_003352"
                     /pseudo
     CDS             5469..5801
                     /gene="ddpX"
                     /locus_tag="OO327_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5815..6711
                     /locus_tag="OO327_003353"
     CDS             5815..6711
                     /locus_tag="OO327_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR6019774.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6708..7682
                     /locus_tag="OO327_003354"
     CDS             6708..7682
                     /locus_tag="OO327_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6019775.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCALTACGDNNQHCTCWYPQQEVISV"
     gene            7675..8601
                     /locus_tag="OO327_003355"
                     /pseudo
     CDS             7675..8601
                     /locus_tag="OO327_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="OO327_003356"
     CDS             complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="OO327_003356"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR6019776.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2261 a         2257 c         2316 g         2408 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tctggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gatgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gcagacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccaa ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtctta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg
     3481 ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa
     3541 aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa
     3601 agccgcagaa attgcccttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt
     3661 ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa
     3721 aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag
     3781 gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga
     3841 aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctcttc
     3901 ggattattcc gccgcagaaa aacttcaggt ctaccatttc tcgattaaca gtattgatat
     3961 cgcgatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct caaaggaaga
     4021 tgaaaactat cgtatcttct cgttactgga aaacgccgaa gaagaaaaag aacggcaaat
     4081 agcctcaata ctttcatggg aaatagatat tatctataaa gtcctgctgg attctgattt
     4141 aggcagcagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc ataaaggtcg
     4201 acattatttt agcggcattg ccgaagtggg ccatatctcc cgtctgattc aggatttcga
     4261 cggtattttc aatcaaacca tgcgtaacac caggattttg aataacagaa gtttgcgggt
     4321 gaaatttcta ttacagataa gaaataccgt atcgcagatt attaccttgc tgcgtgaatt
     4381 gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat tacttaaccg
     4441 ccgtttccta ccgactatct tcaaacgcga aattgcccat gccaaccgga ccggtacacc
     4501 gctgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata cgtggggcca
     4561 taacactggc gatgaaattc tgcgtaaagt ctcattcctc tcgcagaaga gattggtgaa
     4621 atcgaaaata ttgggcgctg ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt
     4681 agccagaata ttcatatccc ggcgttatcc gtgaacttgt cggcgctgca ctttcgcagt
     4741 aatcagctgc ctaatcaggt gtctgatgca atgcaagcct ggggtattga cggccaccag
     4801 ctgacggtgg aaatcacgga aagcatgatg atggaacacg ataccgaaat ctttaagcgc
     4861 attcagatcc tgcgtgatat gggcgtgggt ttatcggtag atgattttgg cacgggcttt
     4921 tccggattat cccgcttagt cagtcttccg gtaacggaaa tcaaaattga caaaagcttt
     4981 gtcgatcgtt gtctgacgga aaaacgcatc ctggccttac ttgaagccat taccagcatt
     5041 gggcaaagcc tcaatttaac cgtcgtggcg gaaggggtcg aaaccaaaga gcaatttgag
     5101 atgctacgta agatccactg tcgcgttatt cagggatatt tcttttcccg cccccttccc
     5161 gccgaagaaa ttccaggctg gatgagcagc gtgttaccgc tgaaaatctg acaaattcct
     5221 ctcgcccgca ctcgcgggtt ttcctttaag gtgacactgt cacttaaata ctgtgatttc
     5281 agctacgatt ccgggagatt ccttccttaa ctttccctct tgcaccaaaa ctgcactacg
     5341 ctgcacaaat gttgatcaaa agttaaaaaa tataacttta agccagcatt ctggcgctta
     5401 tcccggcatg gcatgagatc tgcataagcg gaaagcgcag caatttttgt cttatacagg
     5461 aaccgtttat gtcggatacc accgaactgg ttgatttagc cgtgatcttc cctgatctgg
     5521 agatcgaatt gaaatatgcc tgcgttgata acatcacagg taaagctatt tatcagcaag
     5581 cgcgttgtct gctacataaa gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc
     5641 agctgtcagg cttacaactg gtgatttacg atgcgtatcg cccacaacaa gcacaggcga
     5701 tgttgtggca agcctgccca gacccgcaat atgttgttga tgtgacggtc ggttctaatc
     5761 acagccgtgg cacggcgctc gacctgacgc ttcgtgatga cgtggaggtg gtgaatgatg
     5821 ctaagcgagg aaacgtccgc cgtacgccca caaaaacaaa cgcgatttaa cggtgcaaaa
     5881 ctggtatgga tgctgaaagg cagtccgctc accgtgaccg gcgcagtcat cattgtatta
     5941 atgcgactga tgatgatttt ttcaccgtgg ctggcgacgc atgatcccaa cgccattgat
     6001 ttaaccgccc gccttttgcc gccttctgcg gcgcactggt ttggcaccga tgaagtggga
     6061 cgcgatctgt ttagccgcgt actggtcggc agtcagcaat caattctcgc cggattagtg
     6121 gtggtcgcca ttgcgggtat gattggttcg ctactcggat gtctatccgg tgtgcttggc
     6181 ggacgcgcag acgccatcat catgcgcatc atggacatta tgctgtcgat tccttcgttg
     6241 gtactgacaa tggcactggc tgccgctctc gggccgagtt tatttaacgc catgctggcg
     6301 attgctattg tgcgaattcc cttttatgtg cgcttggcgc gggggcaagc attagttgta
     6361 cgccagtata cctatgttca ggcggcgaaa acctttggcg cgtctcgttg gcatctgatc
     6421 aactggcata ttttacgtaa ctccctaccg ccgctgatcg tgcaggcatc gctggatatc
     6481 ggtagcgcga ttttaatggc cgccacgttg ggatttattg gcctgggtgc tcaacaaccg
     6541 agtgctgaat ggggcgcgat ggtagcgaat ggtcgcaact atgtactcga tcagtggtgg
     6601 tattgcgcat ttccgggggc agcgattttg cttacctccg tcgggtttaa tctctttggt
     6661 gatggtattc gcgatctgct tgacccgaaa gcaggaggaa agcagtcatg acccaacccg
     6721 ttctggacat tcaacagctg catttgagtt tccccggttt taacggtgat gttcacgcgc
     6781 tcaacaatgt gtccttgcag attaaccgcg gcgaaattgt cggtctggtg ggagaatccg
     6841 gctcaggtaa atcagtcacc gcaatgctga ttatgcgtct gctaccgacg ggcagttatt
     6901 gcgtacatcg ggggcagatt tcactgctgg gagaagatgt tcttaacgct cgggaaaaac
     6961 agcttcgtca gtggcgcggc gcacgagtgg cgatgatctt tcaggaaccg atgaccgccc
     7021 ttaatccgac acgtcgaata ggtcttcaga tgatggacgt aatccgccat catcaaccaa
     7081 taagtcgtcg ggaagccaga gctaaagcga ttgcactgct ggaagagatg caaatcccgg
     7141 atgccgtgga agttatgtcg cgctatccgt ttgagctttc aggtggtatg cgccagcggg
     7201 taatgattgc gccggcattc tcctgcgagc cgcaattgat tattgccggc gaaccgacga
     7261 cggcgctgga cgtcacagta cagttgcagg tactgcgttt gcttaaacat aaagcacgcg
     7321 ccagtggaac tgcggtactg ttcatcagcc atgatatggc cgtggtgtcg caactgtgcg
     7381 atagcgttta cgtgatgtat gccggaagcg taattgaaag cggcgggacg gcagacgtta
     7441 tccatcatcc ccggcatccg tataccattg gtttgctgca atgcgcacca gaacatggag
     7501 taccacgcca gccattaccc gccattccag ggacggtacc aaacctcacc catttgcctg
     7561 acggctgcgc ttttcgcgat cgttgctatg cggcaggtgc acagtgtgcg ctgacagcgt
     7621 gtggtgacaa caaccagcac tgcacctgtt ggtatccgca gcaggaggtc ataagtgtct
     7681 gacacgttat taacgttacg cgacgtccat atcaattttc cggcccgtaa aaactggctt
     7741 ggcaaatcta cggaacatgt tcatgccatt aatggtattg atttacagat ccgccgtggt
     7801 gaaaccttag ggatcgtcgg cgagtcaggc tgcggcaaaa gcaccctcgc acagctttta
     7861 atgggcatgc tgcaaccgag ccacgggcag tacatccgtt caggctcaca acgcattatg
     7921 cagatggtgt ttcaggaccc gctctcttcg cttaatccgc gcttaccggt gtggcgcatc
     7981 atcacagaat cgctctggat agctaagcgt agtagtgaac aacagcggcg agcgttggca
     8041 gaggagttgg ctgtgcaggt gggtattcgt ccggagtatc tcgaccgcct gcctcatgcg
     8101 ttctccggcg ggcagcggca acgcatcgcc attgccagag cactctcttc gcagcctgac
     8161 gtgattgtgc ttgatgagcc aacctcggcg ctggatatct ccgtgcaggc gcagatcctc
     8221 aatttgctgg taacgctaca ggaaaatcac gggctgactt atgtgctgat ttcacacaat
     8281 gtctcggtga tacgtcatat gagcgatcgg gtggcggtga tgtatctcgg gcagattgta
     8341 gaactgggcg acgcgcaaca ggtgttgact gcttcggcac atccatacac ccgattattg
     8401 ctggattccc tccccgccat tgataaaccg ctggaggaag aatgagcatt acgtaaaacg
     8461 gatctgccgg gaaaccgcac gttgccgcaa ggctgttttt tctacgaacg ttgccctctg
     8521 gcaacccacg gatgtgaagt ccggcaatca ttagcaataa gggaggacgg acgtgacctc
     8581 cggtgctggc gggcgctgta gagatgctaa caacgcatca ggcattcacg cgcctgacgc
     8641 gtcatccggc aatgctttac gatttcaact ggtaatccag cgtaatttcc gctttcagta
     8701 cctgagagac cgggcatcct gcttttgctt tctggatgat gccgtcaaag gtagaggcat
     8761 caatacccgg caccgcaact tcactcttca gtgcgatttt cgtaatcgca aaaccggcat
     8821 ccactttatc cagcgacaca tcggcggtgg tatcaattga tgttggcgtg aatcccgctt
     8881 cccccagcat taatgaaagc gccattgaga aacatgcggc atgcgctgcg ccaatcagtt
     8941 cttcagggtt ggttcctttt tcgccttcaa aacgcgtgtt aaatccatac ggctgttggt
     9001 tcagcacgcc gctctcggtg gatactgttc ccttcccgcg tttgatatcg ccttcccagt
     9061 gtgcctgacc tttcttatgg attgtcattg ttgctctcct gtgggcttaa aaaggaaatt
     9121 atagactacg tgagaaacag cacgaggata agcaggatat tccgaataaa atacggcgaa
     9181 aatattactt ctgcacttaa attaaattcc cacaatagat aaccgggaat atatatctca
     9241 ag
//
LOCUS       DAJTSI010000151         9209 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000151 DAJTSI010000000
VERSION     DAJTSI010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.151"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..353)
                     /locus_tag="OO327_003357"
     CDS             complement(<1..353)
                     /locus_tag="OO327_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HCR6019777.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRV"
     gene            complement(350..1741)
                     /gene="selA"
                     /locus_tag="OO327_003358"
     CDS             complement(350..1741)
                     /gene="selA"
                     /locus_tag="OO327_003358"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR6019778.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(1839..2447)
                     /locus_tag="OO327_003359"
     CDS             complement(1839..2447)
                     /locus_tag="OO327_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR6019779.1"
                     /translation="MKLVGSYTSPFVRKLFILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(2518..3603)
                     /locus_tag="OO327_003360"
     CDS             complement(2518..3603)
                     /locus_tag="OO327_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6019780.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(3606..3968)
                     /locus_tag="OO327_003361"
     CDS             complement(3606..3968)
                     /locus_tag="OO327_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR6019781.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            4505..6418
                     /gene="mtlA"
                     /locus_tag="OO327_003362"
     CDS             4505..6418
                     /gene="mtlA"
                     /locus_tag="OO327_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR6019782.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            6513..7661
                     /gene="mtlD"
                     /locus_tag="OO327_003363"
     CDS             6513..7661
                     /gene="mtlD"
                     /locus_tag="OO327_003363"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6019783.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            7661..8248
                     /gene="mtlR"
                     /locus_tag="OO327_003364"
     CDS             7661..8248
                     /gene="mtlR"
                     /locus_tag="OO327_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR6019784.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(8260..8469)
                     /locus_tag="OO327_003365"
     CDS             complement(8260..8469)
                     /locus_tag="OO327_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019785.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            8753..9115
                     /locus_tag="OO327_003366"
     CDS             8753..9115
                     /locus_tag="OO327_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR6019786.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         2259 a         2444 c         2365 g         2141 t
ORIGIN      
        1 tccacgcgat cggctttggt cagcgccact gtcagcatcg ggttaccggt cagctgcaaa
       61 atcgccagat gctcacgggt ctgtgccatc acgccgtcat cgcacgccac caccaacagc
      121 gcgtgatcga taccaccaac gcccgccagc atgttggaaa gaaacttttc atgaccggga
      181 acgtcgataa aaccaggcac gcgaccatcc ggctgcggcc agtaggcata gccgagatcg
      241 atggtcatgc cgcgcttttt ttcttccggc agacggtcag catttacgcc agtaatcgcc
      301 tgcaataagg ttgttttgcc gtggtcaacg tgtccggcag tcgcaataat catttcaaca
      361 acatctccaa aaaccgttgc tcatcttcaa ggcagcgtaa atccagccac aatcgtccgt
      421 cataaatacg accaatcacc ggcactggca attcacgcca gcgggcggct aatgactcaa
      481 ggtggctacc gcgtccatca tggggtgtaa acgttaatgc cgcgctcggc aggcgatcaa
      541 ccggcagcga accactgcca atctgcgaaa gacatggcat aacctgtacc gcaaactccg
      601 cgccgtaatg tgcgacaagg ggagcctgta aacgttgtgc ctggatttga atgacctctg
      661 cgctgcgggt aagcaggcgc agggtcggta atttttcact cagagcttca gggtgtaaat
      721 aaagacgcaa cgtggcttcc agcgccgcga gggtcatttt atccgcgcgt aatgcacgct
      781 tcagcgggtg gctttgcagg cgggcgatca tctctttttt accaacgata atcccggcct
      841 gcggcccgcc taacaacttg tcgccggaga agctcaccag actgacgccc gccgcaatca
      901 actcctgcgg cattggctct ttcggcaaac cgtactggct aagatcgacc agcgagccac
      961 tgcctaaatc agtcaccact ggaacatcca gctctttgcc gagcgccacc agttcagctt
     1021 cgtctatcgc tttggtgaac ccctgaatgc tgtagttact ggtatgtact ttcatcaaca
     1081 gtgcggtatt ttcattcacc gcctgacgat aatcattcgc gtgcgtgcgg ttggtggtcc
     1141 ctacttcgtg tagggtgcag cctgcctgac gcataacatc gggaatacga aacgcgccgc
     1201 caatctccac cagttcgccg cgagatacca ccacctcttt cccgctggca gtggccgcca
     1261 acatcaataa caccgccgcc gcattgttat tgacgataca ggcatcttcc gcccccgtaa
     1321 tacggcacag cagctgcgcc agcgcccgat cgcgatgtcc gcgtccggcg tcgtccagat
     1381 cgtactcgag ggtcactggc gaacgcatag cctgcgcaac ggcttccacc gcggcttccg
     1441 cctgtaaagc tcgcccaagg ttggtatgca gcacggttcc cgtcaggttg atcaccggac
     1501 gcagcgcgct ctgcgcctct ttcgtcaacc gggcatcgac ttcttgcgcc cagttttcac
     1561 accacgcagg cagcgtctgg ctgccacgaa tcacttctcg cgcttcgtcg agcatctgac
     1621 gcaacaattc caccacgcgg gtgtgaccat aagtatcacg caaagaaagg aaggagctat
     1681 cgcgcaataa gcgatcaata gccggaagtt gactatagag ggaacgcgtt tcggttgtca
     1741 taggaaacct ggctgatcaa ggccctctca cacggagaag ggcgtttaac ataaccacgg
     1801 attgtaacgt gagatgggtc aggaggacat atcgcgcatc aagcctttgg cggttcggtg
     1861 cgagcaaaac tttcgcggct aaacaggttt tcgaccagtt tgactaagtg tgggcggtcg
     1921 acacaccaac ctggcgcaac gcggcggaaa ttgagatatc cgacagcaca ggcaatggcg
     1981 atagtcgcca gattgaccgt atcggttttg agtgtgccat cgaccagata tccttccagc
     2041 acatccagac tgcggttgat tttctcccgc tggcgtaaca attcatcttc agactgctgc
     2101 gccgccggac gcgcctgttc acgcaccgat accagcccgg catccataat gccatccgcc
     2161 agcgcctcaa ttttgcgcac ccgcaacgac tccagcggat cgcgcggcaa catcgccgga
     2221 gcgacattca ttaactcaat atattcagcg atgatcggcg aatcaaacca gcattcgccc
     2281 tcttcggtca ccagcaccgg cactttccct aacgggttaa attgcgccac gccgttatcc
     2341 gcgttatagg gcagttcatt aataaattcg aaagttatgc ccttttctaa caacagaata
     2401 aaaagtttgc gtacaaacgg gctggtgtag ctaccgacga gtttcatgcc gagtcctttg
     2461 tgcgaggaaa aatatcagta tggctcaggc tcacgcaaaa cacaggtgat ataagtctca
     2521 atgatccaaa taaagataat gcatccaact ggtcattctt agcagcactt tccgcatcac
     2581 cgaaacatgg ctgaaatggt cggagtaaac cgccacctgg gcgtagatgc cgtcgggtaa
     2641 ggcatcgata tcatcgttag ggtccagttc aatggttccc agcacaccgt ccgtgccggg
     2701 aacgaccgtt aatgattgca ataccccctg cgcctgataa gaaccgcctg gcacgacagg
     2761 taaaatacta gtcaatttgc cgtggaacac ctgcccaggt agcgcgttaa acaccacttc
     2821 cgcatcatcg ccaggtttca gacgtaacag cgagttttgc cgaaattggg cgacaatttg
     2881 ccgtttttgc tcggggatga agaccatcac cggacgcagc ggcaaggcag ctgcgtatgt
     2941 acctgggcgg atcagtacct gagtgacgta gccattgctt ggtgcgcgaa tgacagtctg
     3001 ctcaagatta tattttgctt cagtaagttg cgctcttaag ctcacaatct gcgattgctc
     3061 gccgttgacc atactgtcga gctgactctg gatctgcgcc tgctccgcca ccgagccttt
     3121 cactaacgca tcctgcgcga ggaaattttg ccgcgcatcg tcgatgtcac gttccgagaa
     3181 cggattcacc gccgcctggc tgcctttcag gtaacgttga taatttttaa acagacggtc
     3241 gcgctccgct gaaacctggg tggtgttggc ctgcgcttca gtgagctgcg cacgcagcgt
     3301 ctttatatta tgcgtcgccg tcatcaggtc agcctgaagt ctgtcaactc gcgcctggta
     3361 acgaaccggg tcgagcttaa aaagcacctc gcccttttga ataagctgat tattcttgtc
     3421 agtgacttca gtaacaattc ccgtcacctg tggcgtgata gggatcgcta tcactgcctt
     3481 ttgcgcggta aaagtgtaag ggtggttgta gttcatcaac aaaatcaaac cactcaccag
     3541 aaacacgcct cccagcgccg ccgtcgccag cgccacgtaa gttaaaacaa tcaatagatc
     3601 cataattaga tctccgggaa cgtggaatag tcagtctttt tctcggcggc gagttgatgc
     3661 tccagccggg aaattcggtc agaaagtgcg gcgatttccg gttcagtcgc tttctcctgc
     3721 gacgcaacat gtgactgcat accccagcca cgctccggtt gatagagcgt cgcccagatc
     3781 cacagaaacg gccagataac atgcagagta aacaggctca cccagcccgc cgtatgaata
     3841 gcgtcggcat gggggtgatt gcgctttttg gcaatcagat aagggatgtc atgtatcgcg
     3901 atgattccat aaaaaatcac cagaaagacg aagatcagca ctcccaacgc gaaatagttt
     3961 agaaacatat ctgcctcgga ttcacgttta tcagtgttgt tttttggctg gcagccagaa
     4021 gggagtcagg ctgatatttt gacaataatc cgggttcgcg attctcgcca taacaccaaa
     4081 gaataatttt tagaggtgat gagttgctta gttacataac gattgtatga cgaaggcata
     4141 acatgctgta gatcacatca ggtgaacgcc gtaagaaaat atcttgtgat tcagatcaca
     4201 aagattcaac aaaccatcaa aacaaaaatg tgacattact cacatttaaa tgccattttt
     4261 agcgaaaatc accgccttgt ttctttttta tacaggcgtt ttgtgatgag cgtcacgtca
     4321 attacctctc taccccctat atttatgtga ctgatatcac acaaaagccc gtcgactgga
     4381 cagttaaccg attcagtgcc agatttcgca gtatctacaa ggtccggcta cctctgccgc
     4441 cacattaaca aaaaacctcg ggcttccagc ctgcgcgaca gcaaacataa gaaggggtgt
     4501 ttttatgtca tccgatatta agatcaaagt gcaaagcttt ggtcgtttcc tcagcaacat
     4561 ggtgatgcca aatatcggcg cgtttatcgc gtggggtatc atcaccgcgt tatttattcc
     4621 aacagggtgg ttaccgaacg agacgctggc gaagctggtc gggccgatga tcacttatct
     4681 cctaccgctg ctgatcggtt ataccggtgg taagctggta ggcggcaaac gtggtggcgt
     4741 agtcggtgcc attaccacca tgggcgttat cgtcggcgca gacatgccga tgttcctcgg
     4801 ttctatgatt gcaggtccgc tgggcggctg gtgcattaag cacttcgacc gctgggtaga
     4861 cggtaagatc aaatccggtt ttgagatgct ggtgaataac ttctccgcag gcatcatcgg
     4921 gatgatcctc gctattctgg cattcctcgg cattggcccg attgttgaag ccctgtccaa
     4981 aatgctggct gcgggcgtta acttcatggt tgtccatgac atgctgccgc tggcgtctat
     5041 ctttgttgaa ccggcgaaaa tcctgttcct caacaacgcc attaaccacg gtatcttctc
     5101 gccgctgggt attcagcagt cccatgaact gggtaaatca atcttcttcc tgattgaagc
     5161 taacccaggt ccaggtatgg gcgtgctgct ggcgtacatg ttctttggtc gtggtagcgc
     5221 taaacagtct gcgggcggtg cggcaatcat ccacttcctg ggtggtatcc acgaaatcta
     5281 cttcccgtat gtgctgatga atccgcgtct gatcctcgca gtcatcctcg gcggtatgac
     5341 tggcgtgttc acgctgacta tcctgggcgg tggtctggtt tctccggcat ctccgggttc
     5401 tatccttgct gtactggcga tgacaccaaa aggtgcttat ttcgctaaca tcgcgggtgt
     5461 gtgtgcggcg atggctgtct ccttcgttgt ctctgctatt ttgctgaaaa ccagcaaagt
     5521 gaaagaagaa gatgatattg aagcagcaac tcgtcgtatg caggacatga aagctgagtc
     5581 taaaggcaca tctccgctat ctgcgggcga tgtgactaac gacctgagcc acgtacgtaa
     5641 aatcatcgtt gcctgtgacg ccggtatggg ttcaagtgcg atgggcgcag gcgtgctgcg
     5701 taagaaaatt caggatgcag gtctgtcgca gatttcggtc actaacagcg cgatcaacaa
     5761 cctgccgcca gatgtggacc tcgtcatcac tcaccgtgac ctgaccgaac gcgctatgcg
     5821 ccaggttccg caggcacagc atatttcgct gaccaacttc ctcgacagcg gcctgtacac
     5881 cagcctaacc gaacgtctcg ttgctgccca gcgccatact gaaaacgaag tgaaagtgaa
     5941 agacagtctg aaagacagct ttgacgattc cagtgctaac ctgttcaaac tgggcgcgga
     6001 gaacatcttc ctcggtcgca aagcagcaac caaagaagaa gcgattcgtt ttgctggcga
     6061 gcagctggtg aaaggcggtt acgttgagcc ggaatacgtt caggcgatgc tggatcgtga
     6121 aaaactgacc ccgacttatc tgggtgagtc tatcgcggtg ccacacggta cggttgaagc
     6181 gaaagatcgc gtactgaaaa cgggcgtggt gttctgccag tacccggaag gcgtgcgctt
     6241 cggtgaagaa gaagatgaca ttgcccgtct ggtgattggt attgcagccc gtaacaacga
     6301 gcacattcag gttatcacca gcctgaccaa tgcactggat gatgagtccg tcatcgagcg
     6361 tctggcacac accaccagcg tggatgaagt gctggaactg ctggcaggtc gtaagtaatc
     6421 caatcctacc ctctcctcac ggagagggtt tgggtgaggg aaaagcctca ccccagccct
     6481 ctcgggtaaa aacattgatg aaggttaata ctatgaaagc attacatttt ggcgcaggta
     6541 atatcggtcg tggctttatc ggtaaactgc tggcagacgc gggtatccaa ctgacgtttg
     6601 ccgatgtcaa tcaggtggta cttgatgccc tgaatgcccg tcatagctat caggttcatg
     6661 tggtcggtga aaccgagcag gtagataccg tttccggcgt caatgctgtc agcagcattg
     6721 gtgatgatgt cgttgatctg attgctcagg tcgatttagt cactaccgcc gttggcccgg
     6781 ttgtgctgga acgtattgct ccggctatcg ccaaagggct ggtgaaacgc aaagaacaag
     6841 gtaatgaatc cccgctgaac atcatcgcct gtgaaaacat ggtacgcggc accacgcagc
     6901 tgaaaggtca tgtgatgaac gccctgccgg aagacgccaa agcgtgggta gaagagcacg
     6961 ttggctttgt cgattccgcc gttgaccgca tcgtaccgcc ttcggcttcg gcaactaacg
     7021 atccgctgga agtgacggta gaaactttca gcgaatggat tgtcgataaa acgcagttca
     7081 aaggcgcact gccgaacatc ccaggcatgg agttaaccga caacctgatg gcatttgtcg
     7141 aacgtaaact cttcaccctg aacacgggtc atgctataac cgcgtacctc ggaaaactgg
     7201 ccggtcatca gaccattcgt gacgctattc tcgacgagaa aatccgcgcg gtggtaaaag
     7261 gtgcgatgga agaaagtggt gcggtactga tcaagcgcta cggctttgac gcagacaaac
     7321 atgcggcgta catccagaaa atcctcggtc gttttgagaa cccgtatctg aaagatgatg
     7381 tagagcgcgt aggccgtcag ccgctgcgta aactgagtgc tggcgaccgt ctgatcaagc
     7441 cactgctcgg tacgctggaa tacagccttc cgcacaagaa tctgattcag gggattgctg
     7501 gtgcaatgca cttccgcagt gaagatgatc cgcaggctca ggaactggca gcactgatcg
     7561 ctgacaaagg tccgcaggcg gcgctggcac agatttccgg tcttgatgcc aacagcgagg
     7621 ttgtatccga ggcggtaacc gcttataaag caatgcaata atggtggacc aggcgcagga
     7681 caccctgcgc ccgaataaca gattgtcaga tatgcaggca acaatggaac aaacccaggc
     7741 cgttgaaaac cgtgtgcttg agcgtctgaa tgctggcaaa accgtgcgaa gctttctgat
     7801 caccgccgtc gagcttttga ccgaggcggt aaatcttctg gtgcttcagg tattccgcaa
     7861 agacgattac gcggtgaagt atgctgtaga accgttactc gacggcgacg gtccgctggg
     7921 cgatctttct gtgcgtttaa aactcattta cgggttgggc gtcattaacc gccaggaata
     7981 cgaagatgcg gaactgctga tggcattgcg tgaagagcta aatcacgacg gcaacgagta
     8041 cgcctttacc gacgacgaaa tccttggacc ctttggtgaa ctgcactgcg tggcggcgtt
     8101 accaccgccg ccacagtttg aaccagcaga ctccagtttg tatgcaatgc aaattcagcg
     8161 ctatcaacag gctgtgcgat caacaatggt cctttcactg actgagctga tttccaaaat
     8221 cagcttaaaa aaagcctttc aaaagtaagc aacgtctgct tactgcccct ctacctgctt
     8281 cggccgataa agccgacgat aatactccag acgttgtaga tataacggca cgctttcatc
     8341 gggtattccg gacggaatcg cgttacgggg aggaagtttt ttcagatact cccggaacgc
     8401 ctggcttgat gccatgaaat ctacggcttt atcgataaga agcggaacgt tttcacctaa
     8461 tttgcccatg atgatatctc cgtattaccc cgcgccggga atgcgcggcc gccaatttta
     8521 gtttaggttc ccggtaaaga tgacattaag gaaaacgtgc tgaatcctca ataatcatcc
     8581 gctgctgttt attatctttt tctatagatt tcttaattaa tcaacgaatt gcgtagtttt
     8641 tgagcatgtt aatgattacg ttgcagaact attacaatgt catcagttgt gaacaaagca
     8701 cctggtcgcg catactaggg gctataaatt tatctttatc agaagccatc acatgaaaga
     8761 agtcgaaaaa aacgaaatca agcgtctcag cgatcgcctg gacgccatcc gccaccagca
     8821 ggccgatctg tcgctggttg aagccgcaga caaatatgcc gagctggaaa aagagaaagc
     8881 cacgctggaa gcagaaattg ctcgcctgcg tgaggttcat agccaaaaac tgagtaaaga
     8941 agcacagaag ctgatgaaga tgccgttcca gcgcgcaatt accaaaaaag agcaggctga
     9001 tatgggcaag ctgaagaaaa gtgttcgcgg actggtggtt gtgcacccaa tgaccgcgct
     9061 ggggcgcgaa atgggcctgg aagagatgac tggattttct aagactacgt tttaagcccc
     9121 tcagacgact ggttcgatcg tatcaacccg catctggctt gccttagctg gtcaatgcgg
     9181 ggactctcct gtctactcgc ctaattacc
//
LOCUS       DAJTSI010000152         9191 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000152 DAJTSI010000000
VERSION     DAJTSI010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9191
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.152"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..904)
                     /locus_tag="OO327_003367"
     CDS             complement(<1..904)
                     /locus_tag="OO327_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCR6019787.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNV"
     gene            964..1242
                     /locus_tag="OO327_003368"
     CDS             964..1242
                     /locus_tag="OO327_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019788.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="OO327_003369"
     CDS             complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="OO327_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR6019789.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="OO327_003370"
     CDS             complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="OO327_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR6019790.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3396..3728
                     /gene="hdeA"
                     /locus_tag="OO327_003371"
     CDS             3396..3728
                     /gene="hdeA"
                     /locus_tag="OO327_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR6019791.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3844..4170
                     /gene="hdeB"
                     /locus_tag="OO327_003372"
     CDS             3844..4170
                     /gene="hdeB"
                     /locus_tag="OO327_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR6019792.1"
                     /translation="MNILSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4234..4880
                     /locus_tag="OO327_003373"
                     /pseudo
     CDS             4234..4880
                     /locus_tag="OO327_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4922..5452)
                     /locus_tag="OO327_003374"
     CDS             complement(4922..5452)
                     /locus_tag="OO327_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6019793.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5608..6174)
                     /gene="slp"
                     /locus_tag="OO327_003375"
     CDS             complement(5608..6174)
                     /gene="slp"
                     /locus_tag="OO327_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR6019794.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(6421..7644)
                     /locus_tag="OO327_003376"
     CDS             complement(6421..7644)
                     /locus_tag="OO327_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019795.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIEFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            complement(8225..>9191)
                     /locus_tag="OO327_003377"
     CDS             complement(8225..>9191)
                     /locus_tag="OO327_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6019796.1"
                     /translation="EMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGRG
                     YLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTFG
                     WKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKV
                     SCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIY
                     GFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALI
                     IGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT         2681 a         1826 c         1922 g         2762 t
ORIGIN      
        1 atacattctg gcggctacct tcatccacta atgccgtgac atacatgcca ggcagcaagt
       61 caccatttgg gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatcaaccg
      121 tcgggtcgga gaatttcagc gtgccggtct ggctgtaacg tttaccattt tccagattga
      181 gctgtactgg cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca
      241 tgcgtaagaa atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta
      301 cggtaaccag cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt
      361 tcccgctgac gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct
      421 gttcaacagc cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg
      481 cggtgtcgta atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa
      541 aggtgatgcg ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt
      601 ttagctcggc ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt
      661 tatcgccttc gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg
      721 caacttcata aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccagggg
      781 agagtgtgac gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat
      841 cgcaggcggt gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt
      901 tcattttagt ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga
      961 gatatgaggg atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac
     1021 aggcacagtg atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca
     1081 gttcctgcca gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat
     1141 caagtcgttt tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc
     1201 cctcgtcatg ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat
     1261 acccagggtg acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt
     1321 ctgaatcttt aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt
     1381 atttttcaga atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt
     1441 cttatggggc aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg
     1501 gctaacaaaa taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac
     1561 aataattctc tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg
     1621 attgtgatag gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga
     1681 cagggaattg atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag
     1741 aaaagaatct ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa
     1801 catgtcatcc ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc
     1861 ctataagaac tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga
     1921 tttatccatc acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt
     1981 gtaaatagta aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata
     2041 accagtttgt gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc
     2101 gagtctcctt gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg
     2161 aaattacctg agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca
     2221 cggatattta tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt
     2281 taaccgccca tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta
     2341 aaacattaaa cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg
     2401 ccatggagtg aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat
     2461 tatttcaatg atagtgcgca attgatctac aacactgcgt agcggagaga gtattaatcg
     2521 gatcatagtc acatcaagtg actatgatcc gggtgacaac cggggtaatt attgctgctt
     2581 aacgaacaaa ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac
     2641 cagtgtggaa accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat
     2701 gacgatatcc agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg
     2761 ataccaactc atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc
     2821 aatggcaaaa atgcccagct ccggtgcacg gatgaagaaa tagccgatca ataaataggc
     2881 gactgcgacg aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa
     2941 cccgacaata agcgcaatac ccgagcagat taataatgca cccactactg tgcttaaaat
     3001 atcgccagag acgaacggga aactgataca cagcaacccg acgataaaca gcagcacggc
     3061 aataaactgg attgctctgc gatgcttttt cagcatctcc agatcaaact tcaaaattgt
     3121 tgccttatct atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta
     3181 tcaatctatg ccaaaaacgc gtctaagaat gcagtcgatt taataaaaat ttcctaattg
     3241 cagtatctga tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt
     3301 tccatcaaca tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt
     3361 gacggctctt tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc
     3421 ttggtggtct gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg
     3481 ataacaaaaa accggtcaac tcatggacct gtgaagattt cctggctgtg gacgaatcct
     3541 tccagccaac tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg
     3601 cggttttaga tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc
     3661 aggataaaca agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag
     3721 atatgtaatt ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata
     3781 tcttctttaa aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac
     3841 aaaatgaata ttttatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg
     3901 tcactggtga acgcacaatc tgcgttggca gccaatgaat ctgctaaaga tatgacctgc
     3961 caggaattta ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat
     4021 gaagaaacag tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa
     4081 attcctaaag tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa
     4141 aatcaagcat ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt
     4201 gagccatctt gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt
     4261 atactcgcgg caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga
     4321 gcagggttac gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg
     4381 aaatatggtt ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt
     4441 gctgctcagg tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac
     4501 caaaatattg taggtctgac gacggcagcg gatatctggg tgaccgccgc ataggtatgg
     4561 ttattggcag cggtatgtac gaactgggta tttatggttc agtgatgacc ttgttggtgc
     4621 tggaagtctt ccatcaatta accttccgcc tgatgaacaa aaattaccat ttacagctga
     4681 cattagtgaa cggtaatacg gtatcaatgc tcgactggtt taagcagcaa aaaataaaaa
     4741 ctgatttagt ttcgttgcag gaaaatgaag atcatgaagt ggtcgccatt gatatccagc
     4801 ttcatgccac aacgttgata gaagacctgt tgcgattatt aaaaggtatg gcaggggtga
     4861 aaggcgtttc aattagctaa tctgtgtggc agcgtagcca gactcaccgt aagcctgaaa
     4921 ttcacaccag ataatcaata tgctgatggc gtaccagctc attgatccgc ttaagattga
     4981 ggatcatcat gatattgtgt ttatggcagt agaacgtttt tagcgatatt ttcagtttct
     5041 ccagaatatc ttcagttgtc atttgattcg caatgcagct cagaacctga tgttgcttgc
     5101 gcgaaagtga cacattcgct tcaggcacag agcagtaact gccattgatg agtgcgacaa
     5161 aatcaatgat agtgatagtc cgcggaacaa aggtcaccgg tactttcccc agacagcgtt
     5221 taatattaaa gggggagaaa acaatgacat ggacaggttt taatcgttca atttgagtga
     5281 gaaaattaga atgaaataca ttattcatta atgtatcaat aatgacgaag gcattctgat
     5341 gcaacattac gtcgatctct tcttcgttct gtatatgaac gacattacct ttactcagaa
     5401 tgtttttcat cgcggtgaag aacatcgtat ccctggtaat tataagaaac ataaccgact
     5461 cctggtgcgg acattaactt tataatcagt ttgccaaagg taattaacag gacagggctt
     5521 ataataataa tagttagctt actgtatatt aattttatgt aatacatgat gcagatgctg
     5581 agaaagcatc tgcatctttc ggtggtgtta tttgaccagc tcaggtgtta cctgactcac
     5641 cgcattggtg tagtaaggcg caccccagcc cggttccggc cagaatgcac catagccgta
     5701 atcccacagg ttataggtgg tattaaccac ttctctcaaa tgccacacct ggatgccctg
     5761 catattcact tccaggaagt tatacgggac tttattgata aagcctggtt gttcaccctg
     5821 aatggtaccg aggatggtaa caaagtgatt acgatagttc actggatcaa ggaagccgct
     5881 ttgtctggcg agcagtcggc cctgatagtt ggcttcaata tcaggcttcg catagctatc
     5941 caacggtaat acagcgattt ctaacaacgt atccgttttg ccattgataa cgttgataac
     6001 cttcccacca aagcgcgctt gttgaccaac atataacccc ggctggttat gaacagcaac
     6061 aaaacttttt tgaatatcag gttggttatt gcctttgata ttttgcggaa ttgaactaca
     6121 tgcggcaagc aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt tcatgttact
     6181 atccttatca acaaatatta aaaccataat agatatttct ttatagtttt catctgattc
     6241 tgaggtgata tgcctgatag taacgtaaac ctgtctgatg tttatatccc ttctgtttag
     6301 ttcaattccg ttattttggg gcgtggtatg agtatccatc gcccaataat ataacccttt
     6361 gttttaaaat tacataaaat ctatttttat tcaatgatta taaagtgcta ataatcaata
     6421 ttatggtatt atattctgat aatgatttga taagcgaatt cttttgccag atggtatttc
     6481 tatttccacc ccataattta tatctttttc attaggcaga tctctcttta aataaaaggg
     6541 gtatgaaatc ttttcgggtt gaagtgtctc gtaatgaaat tctatgaatt catttttaga
     6601 cattgttgcc catttttgaa ttttattgta tagagagggt gttgtaaact cttgaagttg
     6661 tttataacat atttttttga actcttgctt atattttaat atgtcatcaa atgaaagatt
     6721 cactaccttt gcctctaaga aatcaattat tctcgaagca atttccgctt tcgcgcgtaa
     6781 agagttgtca ttaatagtta acaatgtaga aaacaaacca ttgatttgtt tgtcaagatg
     6841 ttgttcatat ttatgtagtt tggggatggg attttccttg tgtgaaaaaa tagtgttaaa
     6901 taaatttgcg tcagtgaaat ttatattaag tagatcttca aaataaaatt ttgataaggt
     6961 taggactagc ttggacatat cttgccctgt catgttgaaa tcaataaagt cgcatccagc
     7021 aagggcttcc atcctatttt catttttact gtgctcaata tacagcttcg ccatttttaa
     7081 gtacaagctt tgaatgtctc ttatatcagt tggtaagtca gctcgttgcc cagcaaaatt
     7141 tttattatcg gaagaaacgg tttggacact gaatctttcg ggtattcctt cttctacgag
     7201 aattttagtt atgattggat tgttgggttc tggattaacg acaacaatga tttgacatac
     7261 agaatcctta tccttttcgt atctaatgtt atgaatctga ttaagtattg attgtgtctg
     7321 ttgcgtaagt ggtgtcgaac ttatcgtttc aatgttatta ataagactta caagttgaac
     7381 cacaactttt gagtagtctg catttgtata tgtggtgttc gtgtggcatg cattaatttg
     7441 ttggagttgt tgttgcgcta aatcaaattg ttcagggttg tatccttctc cagagaatgt
     7501 ggaattaatg ggttgttgtt cttcccaatt atcacaaggc cacttcggtg tgtctggcgt
     7561 tttaacatta tcggtatttg ttgtgcagtt ataaatatcg ccgtgatatg tatttattat
     7621 acctggggta aagtcaattg acataacatt ccttattcat catctgtttt atgctaatgt
     7681 ttttaaattg gataattaga acaggaatgg attttcttct acacacaata aatataatgc
     7741 gttatatctt cctgatataa ttacctttaa attccctttc atttccaccc ggtaatattt
     7801 gtgtataaaa aaagggcagt gatagtatcg taaaggaaaa cgttaacaac tatcaaaaaa
     7861 cgctcatttt aaaaagttat tatgttctcc gatagtgtgt gaagtaatat ttttgtgatg
     7921 ttttagatgt gtaataatga accaggttaa agtgtattta aaaatgtgtt gtattagcga
     7981 ctgaaatctg ccttttaaaa aggaggaggg tactgagaca aagagtcatt gcttctccga
     8041 taaaatgtca gagaagcatg attacgtggt tattgtctgt tgatgacttg ttgaccgagc
     8101 cgtcatcatt ctcaatgcat tagttaaatc atttgtgatt tttaaggatt atcctgtcta
     8161 tgattttcta cttagaagga tatggcacta actatgcatc ttatagttta ctgctggcat
     8221 ctatttaaaa tagcaattga gtcaaatagc ccatcaagat agccatacct aatataaccg
     8281 tcaggaatgc agctatcatc ggcattctga acattgattt aagtaatatc acttccgtca
     8341 ggcttgcgcc ggcactgccg atgattaatg ccattaatgc tcccagaccc attccttttg
     8401 tcatcagaac agatgccaga gggataaccg cctcagcccg gatatacagc ggaataccga
     8461 caacggcgct taatgggatg gcgaagggat tatctgctcc tgcatgagcg gcaatccacg
     8521 ctgagggaat aaagccataa ataaaagatc ctattaatac tcctaacaaa agatatggca
     8581 gtacatcttt aaactgttgc aacgcatctt tggtggctag ttttatggga ttaatactta
     8641 ctacagcttt ggtactgcaa caggtaacta cggggttctc aatggcttta gccgctggac
     8701 tacaacaact cacttttatg ggcgtatatg tcgttcccgg cgaagttttt gctggagcac
     8761 aacaatttgc tgatgagctt ttactggcaa tgatatgacg ttcaaacccc agagaatcca
     8821 ggataatact ggcaagaacg gagacgccgg ctgcgataat cgcgtacaac aaggtgactt
     8881 tccaaccaaa ggtcatccac attagtccga cgataatggg attaagtaat ggtgaaacaa
     8941 ataaaaaagt gagagtcgga ccaaacccgg ctttcgctga caacaatcca cgtaacatgg
     9001 gtattgtcga gcaactacag aacggggtaa cggctcccaa cagagcagcc aggagatagc
     9061 ctctcccttt tctggccccc atcatctgct ggattttatg gtctggcacc ttttgtctta
     9121 tcaggctgac accggcacta atcactataa acagcaacga aagctcgacg gcgagaaaca
     9181 caaacatctc t
//
LOCUS       DAJTSI010000153         9143 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000153 DAJTSI010000000
VERSION     DAJTSI010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9143
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.153"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..923
                     /locus_tag="OO327_003378"
     CDS             <1..923
                     /locus_tag="OO327_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6019797.1"
                     /translation="GIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPP
                     ALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQ
                     LQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIP
                     FLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVA
                     FISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTT
                     HSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1175..2092
                     /gene="oxyR"
                     /locus_tag="OO327_003379"
     CDS             1175..2092
                     /gene="oxyR"
                     /locus_tag="OO327_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR6019798.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="OO327_003380"
     CDS             complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="OO327_003380"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR6019799.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3796..4446
                     /gene="fabR"
                     /locus_tag="OO327_003381"
     CDS             3796..4446
                     /gene="fabR"
                     /locus_tag="OO327_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR6019800.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4446..4805
                     /locus_tag="OO327_003382"
     CDS             4446..4805
                     /locus_tag="OO327_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR6019801.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="OO327_003383"
     CDS             complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="OO327_003383"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR6019802.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6059..6249
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6314..8158
                     /gene="btuB"
                     /locus_tag="OO327_003384"
     CDS             6314..8158
                     /gene="btuB"
                     /locus_tag="OO327_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCR6019803.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8103..8960
                     /gene="murI"
                     /locus_tag="OO327_003385"
     CDS             8103..8960
                     /gene="murI"
                     /locus_tag="OO327_003385"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR6019804.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2089 a         2210 c         2470 g         2374 t
ORIGIN      
        1 tgggtattgg cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg
       61 cacaaaaaga acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg
      121 ctatcgcgcc tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg
      181 ttatcatcgg cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc
      241 gggcgcagtt tgtcctgact gacgaagaac ggacgtatct ctccgcgtcg gttaaaccgc
      301 gtccacagct gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga
      361 tgattttagg tttctctggc gtcaattata ccggttggct ttacattgcg tggttacccg
      421 gttatttgca agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc
      481 ctttcctcgc ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga
      541 aaaaaggcta cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt
      601 cggcattagg cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta
      661 tctcaatggc gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg
      721 tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg
      781 tttttgcttc ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta
      841 atctggcgct ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta
      901 ttgtcaaaga tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga
      961 ataaaaaaaa gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt
     1021 tgagaaactc tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct
     1081 gtgagcaatt atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat
     1141 cgccatgaac tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct
     1201 ggtggcattg gctgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca
     1261 gccgacgctt agcggccaaa ttcgtaagct ggaagatgag ctgggcgtga tgttgctgga
     1321 gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg
     1381 taccgtgctg cgtgaggtga aagtccttaa agagatggca agccagcagg gtgagacgat
     1441 gtccggaccg ctgcatattg gtttgattcc tacggttgga ccatacctgc taccgcatat
     1501 tatcccgatg ctgcaccaga catttccaaa gctggaaatg tatctgcatg aagcacagac
     1561 ccaccagtta ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt
     1621 gaaagagagc gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat
     1681 ctatgaagat cacccgtggg cgaaccgcga atgcgtactg atggccgatc tggcagggga
     1741 aaaactgctg atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgctt
     1801 tgaagccggg gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa
     1861 catggtggcg gcaggtagcg ggatcacttt attgccagcg ctggctgtgc cgccggagcg
     1921 caaacgcgat ggggttgttt atctgccatg cattaagccg gaaccatgcc gcactattgg
     1981 cctggtttat cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat
     2041 ccgcgcaaga atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta
     2101 acgcagctac ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt
     2161 actcaatagt gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt
     2221 cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca
     2281 aaattttcag cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttgaact
     2341 gggcgcggcc cacttcatac ggcactttca tcgcggtcag ctgctgttcg gttttgccca
     2401 cagagctgat ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgg
     2461 tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg
     2521 ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat
     2581 acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg
     2641 ccagcgaatc ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt
     2701 ttttacccga cttcagatgc atgatcacac cgtcgtcaca gctttcgatc ttctcgtact
     2761 cttcgttgtg acgaatcact acgccactgt tccagaagtg gtaggagaga gaatctgaca
     2821 tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat
     2881 ccataccgcg gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta
     2941 catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg
     3001 tgaaatcaac atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag
     3061 cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa
     3121 agcgagcgtt cccctgcaat atttcacagt gattacgttc gtaaaatccc tgacgcatgc
     3181 gcgtttgttg attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga
     3241 gcagtcggga atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg
     3301 cgtgacggag agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt
     3361 gataacgctc gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt
     3421 cgccgccggg gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag
     3481 ggcttacctg ttcttataca taaaagcaac agaatggtaa cattctatcg cgggtaagcc
     3541 aattgatccc cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt
     3601 acttttgccc tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg
     3661 tttccgctgt acgtaaaaga accggccagt aaatatgttt tattgcgtta ccgttcattc
     3721 acaatactgg agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc
     3781 aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg
     3841 ctggtggaag ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg
     3901 cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac
     3961 gtagacgaac tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg
     4021 cgccaggcgc gtcagcgtat cgccaaaggc gggagtgtga tccgtacctc ggtctccaca
     4081 tttatggagt tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc
     4141 ggcacctccg ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt cattgcggaa
     4201 cttgcggact atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc
     4261 gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa
     4321 caacgtcggc aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct
     4381 tattactggt atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac
     4441 gagtaatgaa acaagcaaat caagatagag gtacgctgct gctggcgttg gttgctggtt
     4501 tatcgattaa tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc
     4561 cgattatttc cctggtgctg acggtttact gcctgcatca acgttatctt aatcgcacca
     4621 tgtcggtagg cttgccgggt ctggcagctg cctgttttat tctcggcgta ctgctgtaca
     4681 gcacggtagt tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactct
     4741 ccgtcattat ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg
     4801 agtaatcggt atcatgccgg atgctggcgc atccggcatg ggttttactt cgcggtcagt
     4861 aatacgccgc actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttcg
     4921 accttgtgcg tctggcttaa tgtttccaga tttttgcata acgtttccgg attgcaggag
     4981 atatacagaa tacgtggata cgcctgcacc attttctcgg tttcactgtc cagaccgctg
     5041 cgcggtgggt cgacaaaaat ggtttcgaac tgatagctct ttaagtcgat cccttgcagg
     5101 cggttaaact cgcgcacacc attcatcgcc tgagtaaatt cttctgccgc catacgaata
     5161 atttgtacgt tatcaatatg gttagcggca atgttgtatt gcgcggcagc gactgacggt
     5221 ttggcgattt cggtggctaa tacccgatca aaattgcgcg ccagcgctaa tgaaaagtta
     5281 ccgttgccgc agtacagctc cagtaaatcg ccttttgagc ctttggttac gtccagcgcc
     5341 cattccagca tctgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga
     5401 cgatagatca tctctttccc tgcgaccggc agacgctcgt cgatgtaatc ttgatccagc
     5461 gcgattttgg ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgcgcgcagt
     5521 gcatcgcgca gtgcttccgc ttcctgacgc cactcatcat ccagcttttt atggtacagc
     5581 agggaaacca ccgcctgatt actcagcgtg gtgaggtaat caatctggaa caacttgtgg
     5641 cgcagaacgg gattattacg cacacccgca atcatcgccg tcatcaactg gttgatgagt
     5701 tcactggcgg cggggaagct atccacgcgg atgcggcttt tggtttgttg atcgaaaatg
     5761 atgtgataca ggtcatcgcc atcgtgccag atgcggaact ccgcgcgcat ccggtaatga
     5821 ctgaccggcg agcgaaacac ttccggaacc aggtcagaaa acggtgccat catactttgc
     5881 aaacgtacca ctttttcggc taactgcgct tcatactgtt ctgttggaag gtgttcgggg
     5941 gtcatgatgt atcctgaaaa attaaagtac gcggcgattg tagggattgc tcatcagatg
     6001 tccagatctt gatgaattcc tatttgtgag ctacgtctgg acagtaactt gttacaacct
     6061 gtagcatcca cttgccggtc ctgtgagtta atagggaatc cagtgcgaat ctagagctga
     6121 cgcgcagcgg taaggaaagg tgcgatgatt gcgttatgcg gacactgcca tccggtggga
     6181 agtcatcatc tcttagtatc ttagataccc ctccaagccc gaagacctgc cggccaacgt
     6241 cgcatctggt tctcatcatc gcgtaatatt gatgaaacct gcggcatcct tcttctattg
     6301 tggatgcttt acaatgatta aaaaagcttc gctgctgacg gcgtgttccg tcacggcatt
     6361 ttccgcttgg gcacaggata ccagcccgga tactctcgtc gttactgcta tccgttttga
     6421 acagccgcgc agcactgtgc ttgcaccaac caccgttgtg acccgtcagg atatcgaccg
     6481 ctggcagtcg acctcggtca atgatgtgct gcgccgtctt ccgggcgtcg atatcaccca
     6541 aaacggcggt tcaggtcagc tctcatctat ttttattcgc ggtacaaatg ccagtcatgt
     6601 gctggtgtta attgatggcg tccgcctgaa tctggcgggg gtgagtggtt ctgccgacct
     6661 tagccagttc cctattgcgc ttgtccagcg tattgaatat atccgtggac cgcgctccgc
     6721 tgtttatggt tccgatgcaa taggcggggt ggtgaatatc atcacgacgc gcgatgaacc
     6781 cggaacggaa atttcagcag ggtggggaag caatagttat cagaactatg atgtctctac
     6841 gcagcaacaa ctgggggata aaacacgggt aacgctgttg ggcgattatg cccatactca
     6901 tggttatgat gttgttgcct atggtaatac cggaacgcaa gcgcagacag ataacgatgg
     6961 ttttttaagt aaaacgcttt atggggcgct ggagcataac tttactgatg cctggagcgg
     7021 ctttgttcgc ggctatggct atgataaccg taccaattat gacgcgtatt attctcccgg
     7081 ttcaccgttg ctcgataccc gtaaactcta tagccaaagt tgggacgccg ggctgcgcta
     7141 taacggcgaa ctgattaaat cacaactcat taccagctat agccatagca aagattacaa
     7201 ctacgatccc cattatggtc gttatgattc gtcggcgacg ctcgatgaga tgaagcaata
     7261 caccgtccag tgggcaaaca atgtcatcgt tggtcacggt agtattggtg cgggtgtcga
     7321 ctggcagaaa cagactacga cgccgggtac aggttatgtt gaggatggat atgatcaacg
     7381 taataccggc atctatctga ccgggctgca acaagtcggc gattttacct ttgaaggcgc
     7441 agcacgcagc gacgataact cacagtttgg tcgtcatgga acctggcaaa ccagcgccgg
     7501 ttgggaattc atcgaaggtt atcgcttcat tgcttcctac gggacatctt ataaggcact
     7561 aaatctgggg caactgtatg gcttctacgg aaatccgaat ctggacccgg agaaaagcaa
     7621 acagtgggaa ggcgcgtttg aaggcttaac cgctggggtg aactggcgta tttccggata
     7681 tcgtaacgat gtcagtgact tgatcgatta tgatgatcac accctgaaat attacaacga
     7741 agggaaagcg cggattaagg gtgtcgaggc gacagccaat tttgataccg gaccactgac
     7801 gcatactgtg agttatgatt atgtcgatgc gcgcaatgcg attaccgaca cgccgttgtt
     7861 acgccgtgct aaacagcagg tgaaatacca gctcgactgg cagttgtatg acttcgactg
     7921 gggtattact tatcagtatt taggcactcg ctatgataag gattactcat cttatcctta
     7981 tcaaaccgtt aaaatgggcg gtgtgagctt gtgggatctt gcggttgcgt atccggtcac
     8041 ctctcacctg acagttcgtg gtaaaatagc caacctgttc gacaaagatt atgagacagt
     8101 ctatggctac caaactgcag gacgggaata caccttgtct ggcagctaca ccttctgaac
     8161 cacgtcccac cgtgctggtg tttgactccg gcgtcggtgg gttgtcggtc tatgacgaga
     8221 tccggcatct cttaccggat ctccattaca tttatgcttt cgataacgtc gctttcccgt
     8281 atggcgaaaa aagcgaagcg tttattgttg agcgagtggt ggcaattgtc accgcggtgc
     8341 aagaacgtta tccccttgcg ctggctgtgg tcgcttgcaa caccgccagt accgtttcac
     8401 ttcctgcatt acgcgaaaag ttcgacttcc cggttgttgg tgtcgtgccg gcgattaaac
     8461 ctgctgcacg tctgacggca aatggcattg tcggattact ggcaacccgc ggaacagtta
     8521 aacgttctta tactcatgag ctgatcgcgc gtttcgctaa tgaatgccag atagaaatgc
     8581 tgggctcggc agagatggtt gagttggctg aagcgaagct gcatggcgaa gatgtttctc
     8641 tggatgcact aaaacgtatc ctacgcccgt ggttaagaat gaaagagccg ccagataccg
     8701 ttgtattggg ttgcacccat ttccctctac tacaagaaga actgttacaa gtgctgccag
     8761 agggaacccg gctggtggat tctggcgcag cgattgctcg ccgaacggcc tggttgttag
     8821 aacatgaagc cccggatgca aaatctgccg atgcgaatat tgccttttgt atggcaatga
     8881 cgccagaagc tgaacaatta ttgcccgttt tacagcgtta cggcttcgaa acgctcgaaa
     8941 aactggcagt tttaggctga tttggttgaa tgttgcgcgg tcggaaaatt attttaaatt
     9001 tcctcttgtc aggccggaat aactccctat aatgcgccac cactgacacg gaacaacggc
     9061 aaacacgccg acgggtcagc ggggttctcc tgagaattcc ggcagagaaa gcaaaaataa
     9121 atgcttgact ctgtagcggg aaa
//
LOCUS       DAJTSI010000154         9029 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000154 DAJTSI010000000
VERSION     DAJTSI010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.154"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="OO327_003386"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="OO327_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR6019805.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="OO327_003387"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="OO327_003387"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR6019806.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="OO327_003388"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="OO327_003388"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR6019807.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="OO327_003389"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="OO327_003389"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR6019808.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4466..5590
                     /gene="dprA"
                     /locus_tag="OO327_003390"
     CDS             4466..5590
                     /gene="dprA"
                     /locus_tag="OO327_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR6019809.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5562..6035
                     /locus_tag="OO327_003391"
     CDS             5562..6035
                     /locus_tag="OO327_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR6019810.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6064..6606
                     /locus_tag="OO327_003392"
     CDS             6064..6606
                     /locus_tag="OO327_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HCR6019811.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6611..7183
                     /gene="tsaC"
                     /locus_tag="OO327_003393"
     CDS             6611..7183
                     /gene="tsaC"
                     /locus_tag="OO327_003393"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR6019812.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7188..8006
                     /gene="aroE"
                     /locus_tag="OO327_003394"
     CDS             7188..8006
                     /gene="aroE"
                     /locus_tag="OO327_003394"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR6019813.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            8003..8260
                     /locus_tag="OO327_003395"
     CDS             8003..8260
                     /locus_tag="OO327_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR6019814.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8236..8790)
                     /locus_tag="OO327_003396"
     CDS             complement(8236..8790)
                     /locus_tag="OO327_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR6019815.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2259 a         2192 c         2252 g         2326 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 agggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaatg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacaacgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact tgattgacag catcactgac caatcgcaaa gattgctaag
     4441 gcttgcttat ggcagggaga taaggatggt cgatatagaa atttggctgc gtttaatgag
     4501 catcagcagc ttgtacggcg atgatatggt ccgtatcgct cactggctgg caaaacagtc
     4561 gcatattgat gcggttgtat tgcagcaaac agggcttaca ttgcggcagg cacaacgctt
     4621 tctttcattt ccacgaaaga gtatcgaaag ctcactttgt tggttggagc aacccaacca
     4681 tcatttaatc cctgcggaca gcgaatttta tcctcctcaa cttcaggcga cgacagatta
     4741 ccccggcgca ctgtttgttg aaggagaact gcacgcgctg cattcatttc agcttgccgt
     4801 agtggggagt cgggcgcatt catggtatgg cgagcgatgg gggcgattat tttgcgaaac
     4861 tctggcgacg tgtggagtga caattacgag tggactggcg cgtggaatcg atggtgtggt
     4921 gcataaagcg gccttacagg taaatggcgt cagcattgct gtattgggga atggacttaa
     4981 taccattcat ccgcgtcgcc atgcccgact ggctgccagt ctgtttgaac agggtggcgc
     5041 tctcgtctcg gaatttcccc tcgatgttcc accccttgct tacaatttcc cacgaagaaa
     5101 tcgcattatc agtggtctaa gtaaaggtgt actggtggtg gaagcggctt tgcgcagtgg
     5161 ttcgctggtg acagcacgtt gtgcgcttga gcaggggcgt gaagtttttg ccttgccagg
     5221 tccaataggg aatccgggaa gcgaagggcc tcactggtta ataaaacaag gtgcgattct
     5281 tgtgacggaa ccggaagaaa ttctggaaaa cttgcaattt ggattgcact ggttgccaga
     5341 cgcccctgaa aattcatttt attcaccaga tcagcaagac gtggcattgc catttcctga
     5401 gctcctggct aacgtaggag atgaggtaac acctgttgac gtcgtcgctg aacgtgccgg
     5461 ccaacctgtg ccagaggtag ttactcaact actcgaactg gagttagcag gatggatcgc
     5521 agctgtaccc ggcggctatg tccgattgag gagggcatgc catgttcgac gtactaatgt
     5581 atttgtttga aacctatatt cacactgaag ctgagttgcg tgtggatcaa gacaaacttg
     5641 aacaggatct taccgacgca ggttttgagc gagaagatat ctacaatgcc ctgctatggc
     5701 tggaaaaact tgctgattat caggaagggt tggcagaacc gatgcaactg gcctcggatc
     5761 ctctctccat gcgtatttat acaccggaag agtgtgaacg actggatgcc agctgccgtg
     5821 ggtttctgct tttccttgag cagattcagg tgctcaacct tgaaactcgt gaaatggtga
     5881 tagagcgagt gctcgcgctg gataacgcag agttcgaact ggatgatctg aaatgggtga
     5941 tcctgatggt gttgttcaac atcccgggct gcgaaaatgc gtaccagcaa atggaagaat
     6001 tactctttga agtgaatgaa ggtatgctgc attaatcatt tctttaattc agcataagtt
     6061 gttatggcaa aatcagcact gttcacggtg cgtaataatg agtcctgccc aaagtgcggg
     6121 gctgaactgg ttattcgatc cgggaaacac ggtccgtttc ttggatgctc acagtatccg
     6181 gcgtgtgact acgtccgtcc tctgaaatct tcagcggatg gacatattgt caaagttctg
     6241 gaggggcagg tttgccccgt atgtggcgca aatctggtat tacgccaggg acgctttggt
     6301 atgtttattg gttgcagtaa ctaccctgaa tgcgaacata ccgaacttat cgataaaccg
     6361 gacgaaacag caattacatg cccccaatgt cggacgggcc atctggtcca gcgccgctcc
     6421 cgttatggca aaacatttca ctcctgtgat cgctacccgg agtgtcaatt tgccattaac
     6481 ttcaaaccaa tagctggaga atgccctgag tgtcattatc cgctactcat cgaaaagaaa
     6541 accgcgcagg gtgtaaaaca cttttgtgcc agtaaacaat gtggaaagcc ggtttcggcg
     6601 gaataataac gtgaataata acctgcaagg agacgctatc gcagctgcga tagatgtcct
     6661 caatgaaaaa cgtgtcatcg cctatccaac ggaagccgtt ttcggtgtcg ggtgcgatcc
     6721 tgatagcgaa acagcagtga tgagactgtt ggagttaaaa cagcgtccgg ttgataaggg
     6781 gctgatttta atcgcggcaa attacgagca gcttaaaccc tatattgatg acaccatgtt
     6841 gactgacgtg cagcgtgaaa ccattttttc ctgctggcca ggtcctgtca cctttgtctt
     6901 tcccgcgcct gcgacaacac cgcgctggtt gacgggccgc tttgattcgc ttgctgtacg
     6961 agtcaccgac catccgttgg tggttgcttt gtgccaggct tatggtaaac cgctggtttc
     7021 taccagtgcc aacttgagtg gattgccacc ttgtcgaaca gtagacgaag ttcgcgcaca
     7081 atttggcgcg gcgttcccgg ttgtgcctgg tgaaacgggg gggcgtttaa atccttcaga
     7141 aatccgcgat gccctgacgg gtgaactgtt tcgacagggg taacataatg gaaacctatg
     7201 ctgtttttgg taatccgata gcccacagca aatcgccatt cattcatcag caatttgctc
     7261 agcaactgaa tattgaacat ccctatgggc gcgtgttggc acccatcaat gatttcatca
     7321 acacactgaa cgctttcttt cgtgctggtg gtaaaggtgc gaatgtgacg gtgcctttta
     7381 aagaagaggc ttttgccaga gcggatgagc ttactgaacg ggcagcgttg gctggtgcag
     7441 ttaataccct caagcggtta gaagatggac gcctgctggg tgacaatacc gatggtgtag
     7501 gcttgttaag cgatctggaa cgtctgtctt ttatccgccc tggtttacgt attctgctta
     7561 tcggcgctgg tggagcatct cgcggcgtac tactgccact tctttccctg gactgtgcgg
     7621 tgacaatcac taatcggacg gtatcccgcg cggaagagtt ggctaaattg tttgcgcaca
     7681 ctggcagtat tcaggcgttg ggtatggacg aactggaagg tcatgagttt gatctcatta
     7741 ttaatgcaac atccagtggc atcagtggtg atattccggc gatcccgtca tcgctcattc
     7801 atccaggcat ttattgctat gacatgttct atcagaaagg aaaaactcct tttctggcat
     7861 ggtgtgagca gcgaggctca aagcgtaatg ctgatggttt aggaatgctg gtggcacagg
     7921 cggctcatgc ctttcttctc tggcacggtg ttctgcctga cgtagaacca gttataaagc
     7981 aattgcagga ggaattgtcc gcgtgaatca ggccatccag tttccggaca gggaagagtg
     8041 ggacgagaat aaaaaatgtg tatgttttcc cgctctcgtg aatggtatgc aactgacatg
     8101 cgcgatctct ggcgagagtc tggcgtatcg ctttactgga gatacgccag aacagtggtt
     8161 agcgagtttt cgtcagcatc gctgggacct ggaagaagaa gcggaaaact taattcagga
     8221 acaaagtgaa gatgatcaag gctgggtctg gttaccctga tccagatatt cgtccttcca
     8281 tttcacgtaa ttattcgcgg aatagcgtaa cccagccttc tcttcatcac ttaacgggcg
     8341 gatctgtttg acggggctac cgagatacag atatccgctc tccagccgtt tattttgtgg
     8401 gaccagacta cccgcaccaa tcatcacatc atcttctact attgcgccat caagtaaaat
     8461 tgagcccatc ccaaccaaaa ctcgattgcc aatggtgcag ccgtggagca tcaccttgtg
     8521 accaacagtg acatcttcgc caatggttaa tgggttgcca tctgggttgt acgaggattt
     8581 atgagtgaca tgcaacatac tgccatcctg gatattggtg cgtgctccga tctgtacata
     8641 atgtacatct ccacgaatca caacgagcgg ccagatcccc acatcatcag ccagacgaac
     8701 gtcaccaatc acgacactgc tatcgtcgat cattacgcgc tgaccgattt gtggaaaaag
     8761 atcgcggtat gggcgtaaaa catcagacat acttacctca gcaataaatg atttactaat
     8821 gactttgggg gcattattgg ccttgtgcaa gtcttttagt atgcaaaaaa gcaccgtttt
     8881 gtgtgcgatt gcagcaaaaa gggtgaaaaa acaacaaaca gaaaagaaga tcaaaaaaat
     8941 acttgtgcaa aatattggga tccctataat gcgcctccgt tgagacgaca acgtgaaaca
     9001 cttcacagga tggtcggaac aacgaagag
//
LOCUS       DAJTSI010000155         8956 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000155 DAJTSI010000000
VERSION     DAJTSI010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8956
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.155"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..276
                     /locus_tag="OO327_003397"
     CDS             <1..276
                     /locus_tag="OO327_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCR6019816.1"
                     /translation="NNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSSE
                     LHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            286..876
                     /locus_tag="OO327_003398"
     CDS             286..876
                     /locus_tag="OO327_003398"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCR6019817.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            887..1381
                     /gene="rutF"
                     /locus_tag="OO327_003399"
     CDS             887..1381
                     /gene="rutF"
                     /locus_tag="OO327_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCR6019818.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            1402..2730
                     /gene="rutG"
                     /locus_tag="OO327_003400"
     CDS             1402..2730
                     /gene="rutG"
                     /locus_tag="OO327_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCR6019819.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(2812..2919)
                     /locus_tag="OO327_003401"
     CDS             complement(2812..2919)
                     /locus_tag="OO327_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019820.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            3292..3888
                     /gene="wrbA"
                     /locus_tag="OO327_003402"
     CDS             3292..3888
                     /gene="wrbA"
                     /locus_tag="OO327_003402"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR6019821.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            3909..4136
                     /locus_tag="OO327_003403"
     CDS             3909..4136
                     /locus_tag="OO327_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCR6019822.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(4174..5415)
                     /gene="agp"
                     /locus_tag="OO327_003404"
     CDS             complement(4174..5415)
                     /gene="agp"
                     /locus_tag="OO327_003404"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCR6019823.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(5708..6964)
                     /locus_tag="OO327_003405"
     CDS             complement(5708..6964)
                     /locus_tag="OO327_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HCR6019824.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     NENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            7225..8145
                     /gene="cbpA"
                     /locus_tag="OO327_003406"
     CDS             7225..8145
                     /gene="cbpA"
                     /locus_tag="OO327_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR6019825.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            8145..8450
                     /gene="cbpM"
                     /locus_tag="OO327_003407"
     CDS             8145..8450
                     /gene="cbpM"
                     /locus_tag="OO327_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR6019826.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(8543..>8956)
                     /gene="torD"
                     /locus_tag="OO327_003408"
     CDS             complement(8543..>8956)
                     /gene="torD"
                     /locus_tag="OO327_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR6019827.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
BASE COUNT         2187 a         2211 c         2204 g         2354 t
ORIGIN      
        1 aataatttac tacgtcgact taacgccctc aaacgcgctg actttagtca ccatgcggat
       61 cgcatccgct gcccggtgca aatcatctgc gccagtgatg atctgctggt gccaacagca
      121 tgttccagtg aacttcatgc cgccctgccc gatagccaga aaatggtgat gccctatggc
      181 ggacacgcct gcaacgtgac cgatcccgaa acgtttaatg ctctgttact caacgggctt
      241 gccagcctgt tacatcaccg tgaagccgcc ctgtaaggaa ttgctatgaa cgaagccgtt
      301 agcccaggtg cgcttagcac cctgttcacc gatgcccgca ctcacaacgg ctggcgggag
      361 acacccgtca gcgatgagac gttacaggag ctttatgccc tgatgaaatg ggggccgaca
      421 tcagctaact gttctccggc acggatcgtg tttatccgca ctgcagaagg aaaagaacgt
      481 ctgcgcccgg cgctttccag cggcaatctg caaaaaaccc tgaccgcgcc cgtcaccgct
      541 atcgtcgcct gggacagtga attttatgaa cggttaccac tactgtttcc ccacggtgat
      601 gcccgcagtt ggtttacctc cagcccacaa cttgccgaag aaacagcgtt tcgcaacagt
      661 tccatgcagg cggcttatct gatcgtcgct tgccgtgcgc tgggactgga taccggcccg
      721 atgtcgggtt ttgaccgtca acacgttgac gacgcctttt ttgcgggcag cacgctgaag
      781 agcaatctgc tgattaatat cggctatggc gatagcagca aactttttgc gcgcctgcca
      841 cgtctgtcct ttgaagaagc ctgcgggctg ttgtaaggag caaactatga acatagttga
      901 tcaacaaacc tttcgcgatg cgatgtcctg tatgggcgcg gcggtcaata tcatcaccac
      961 ggacggtcca gccgggcgcg ccgggttcac cgccagcgcc gtctgcagcg tgaccgacac
     1021 gccgcccaca ttactggtgt gcctgaatcg tggggcgtcc gtctggccgg tattcaatga
     1081 aaatcgaacg ctgtgtgtaa atacactgag cgccgggcag gagccgcttt caaatctttt
     1141 tggcggcaaa acgcccatgg aacaccgctt tgccgccgcc cgctggcaga cgggtgtgac
     1201 cggatgcccg caactggaag aggctctggt ttcgtttgac tgccgtatca gccaggtggt
     1261 gagcgtcggc acccacgaca ttctgttttg cgccatcgaa gcgattcatc gtcacaccac
     1321 accctacggg ctggtgtggt tcgatcgcag ttatcacgcg ctgatgcgcc ctgcttgtta
     1381 acctccttaa ggagacagct gatggcaatg ttcggttttc ctcactggca gttaaaatcg
     1441 acctctacag agagcggcgt ggtcgcgccg gatgaacgat tgccgtttgc tcagacggca
     1501 gtaatgggcg ttcaacacgc ggtggcgatg tttggcgcga cggtgttaat gccgattctg
     1561 atggggctgg atcccaatct ttccatttta atgtcgggga tcggcacgtt actgtttttc
     1621 tttattaccg gcgggcgcgt ccccagttat ctcggctcca gcgcggcttt tgtcggcgtg
     1681 gtgattgccg cgacgggctt taacggtcag ggcattaacc cgaatatcag catcgcgctg
     1741 ggcgggatta tcgcctgtgg gctggtttat accgtcattg gcctggtggt gatgaaaatt
     1801 ggtacacgct ggattgaacg actaatgccg ccggtggtga cgggtgcggt ggtgatggcg
     1861 attggcctga acttagcgcc tatcgcagtg aaaagtgtat cggcttcggc ctttgatagc
     1921 tggatggcgg taatgacggt gctgtgtatt gggctggtgg cggtgtttac ccgtggcatg
     1981 atccagcggt tattgattct cgtaggatta attgtcgcct gtctgctgta tggcgtgatg
     2041 accaacgttt tagggctggg aaaagccgtc gattttactc tcgtcagcta tgctgcctgg
     2101 ttcggtctgc cgcatttttc cactcctggt tttaatggcc aggcgatgat gttgattgcg
     2161 cccgtggcgg tgattctggt ggcggaaaac ttaggtcatc tcaaggcggt cgccgggatg
     2221 accggacgca atatggaccc gtatatgggg cgagcattcg tcggtgatgg tctggcaacg
     2281 atgctttccg gctctgtcgg cggtagcggc gtcaccacct atgctgaaaa tatcggtgtg
     2341 atggccgtga caaaagtcta ctcgacgctg gtgtttgttg cagctgccgt catcgctatg
     2401 ctgttgggct tttcgccgaa atttggcgcg ctgatccata ccattccggc cgcggtgatt
     2461 ggtggtgcat caattgttgt gttcgggctg attgccgtcg caggcgcaag gatatgggta
     2521 caaaaccgtg tcgatttaag ccagaacgct aatttgatta tggtcgcagt aaccttcgtg
     2581 ctgggcgcgg gtgattttgc tctcacgctt ggcggtttta cgttgggagg gatcggtaca
     2641 gcaacctttg gcgcgatttt actcaatgcg ttattaagca gaaggttggt tgacgttcca
     2701 ccgccagaag ttgttcatca ggagccataa acagaaaacc gccgggttac cggcggtgat
     2761 gactttttga cgagcttaag aaaaaaatgc attcgtcatt gattagcccg gctagttgtc
     2821 gcttttgccg tgactgctct tgccaccttt tttacctgct tctgatgctc tttcgcggtc
     2881 ttctgcaaaa ttgccggaac cgcctcgatg gtttgccatt actgacctcc gtctgggtat
     2941 agacatcata ttgcatagaa ttaacgcggc gtatggcggc tgccatattt tcgctacgtg
     3001 ggactaagct tagtgaactg acgcggacag acccgcgttt ggtaatatcc tgcaacagct
     3061 tcttagcgag aatagatatc atctccaata attatccata agccgctctt atgattaaaa
     3121 aaacgcgatg agataaaaat gtgtcatttt gcgacaaaat tacgtgcttg tgaaagttgt
     3181 tataaatcaa ataagtggtt gtgaaatttg cactctgaaa aggacgtctt atctttaaat
     3241 aagtggtagc gaatcgctac ggaatagaga taacacgagg agtggttaga aatggctaaa
     3301 gttctggtgc tttattattc catgtacgga catattgaaa cgatggcacg cgcagtcgct
     3361 gagggtgcaa gcaaagtgga tggcgcagaa gttgtcgtta agcgtgtacc ggaaaccatg
     3421 ccgccgcaat tatttgaaaa agcaggcggt aaaacgcaaa ctgcaccggt tgcaaccccg
     3481 caagaactgg ccgattacga cgccattatt tttggtacac ctacccgctt tggcaacatg
     3541 tccggtcaaa tgcgtacctt cctcgaccag acgggcggcc tgtgggcttc cggcgcacta
     3601 tacggaaaac tggcgagcgt ctttagttcc accggtactg gcggcggtca ggaacaaacc
     3661 atcacatcca cctggacgac ccttgcgcat cacggcatgg tgattgtccc cattggctac
     3721 gcagcgcagg aattatttga cgtttcacag gttcgcggcg gtacgccgta cggcgcaacc
     3781 accatcgcag gcggtgacgg ctcacgccag ccaagccagg aagaactgtc tattgctcgt
     3841 tatcaagggg aatatgtcgc aggtctggca gttaaactta acggctaatc ttcaacagga
     3901 ggatacgcat gccaactcaa gaagcgaaag ctcatcacgt cggtgaatgg gcatctctgc
     3961 gcaatacgtc gccggaaata gccgaggcca ttttcgaagt cgccgggtac gacgaaaaaa
     4021 tggcagaaaa gatttgggaa gaaggtagtg atgaagtgtt agtcaaagcc tttgctaaga
     4081 ccgataaaga ttcgcttttt tggggcgaac agaccatcga acgtaaaaac gtttaaccag
     4141 cgactccccc gcttctcgcg ggggagtttt ctgttatttc accgcctcat tcaacacgct
     4201 atcaaactta tccatcgggc agaaaccgtt ggcatctatc gggcaaccgc ttaattccag
     4261 cgtcacgcgc tgcgcaggtg cctgcagggt taacgcatcg gcattacgta actgttccgc
     4321 actctggtac acatattcaa ttttcatcaa atcgcgattg gctttgctgt catgccaacg
     4381 ctggaaaacg attttgccgc caatcggcgt gcgttcgttc tggtcatgca actgatacgg
     4441 tttgaaatcc agcgccgtta acagagaggc tatgttggag tcgtgcccaa ctaacaccgt
     4501 gattttcgga gcactggcgc gatcggtgac cagggctttg tcgatataac tgaccagcgg
     4561 tttcgcaaca ttgcgcgcca cttccggtga ggtaaacaga ctgtcctggt aaccgttttt
     4621 cagcttcgac aataccttcc actgctggtc agatttgatt tctccccagg ccacctgatc
     4681 catcggaaaa ccttcgtagt attgcaaagt aaacgcatct accagcgagt tgccgacttt
     4741 cagcggcccg gaaacacctg gttcttgttg atacttggcg ctaaaggtat ttttgccatc
     4801 caccagcgaa cactgttgtt tctctttaca ggcaggggaa tctttatagt taacgatttt
     4861 ttccagtagc tggtagctgt cggtaagctg gagtttgctg agctctttct ccattgccgc
     4921 caccgcctgt tcactgaatg cggcggaatc atcggtgatc atcgggttaa aggttgggtc
     4981 catagtgccc attttttcct ggtgatgcac aggaatatca cacccaggga atgcaccagt
     5041 aataaagaac tgggcggtag cgacggtacg ttgcagacta ttggcatagg cataaacggt
     5101 gtccggcggc gggcattccc ccgatttcac tatcccctgc tctgccagcc attcacgcat
     5161 gtaatggccc atatacactt cgagcacgcc acctttggtg gtgagttgcc caccggggac
     5221 gtcccattcg ggccatttat tcggcgtcga ctgctccagc acactgccat tgttcgccag
     5281 cggcgcacgt aagttatggc ggctcatcat gagcacttgc tgtagctgat agccttccgg
     5341 tacggtttgt gcctgagcat ttgaagcgag taaaactatc cctgccacag ttgcggcgat
     5401 tagcgttttg ttcattcctg gcacctcttt tgttatcaat cattaaagag tgtgacagaa
     5461 atatgacttt tttccgggaa caggttcgca aaatggcttg ttgaacgcct gtttttctat
     5521 atacatcttc aaaaacaggc aaggtttgac gcaaactatg cctgattagc ccctgcctcg
     5581 cgtacgccgc tgataattgc acctgtggct aaacagaata tctgcggcgt taccaataca
     5641 gagacaatat ttaccaataa aaaagtgtga gactaataac aatggttttt agcatatgcc
     5701 tttgccatta agttgcataa cgatttaaat agtcaagttg cgcattcatt tccttatttt
     5761 ttaacttata agcctgagaa aaaagttcag gagggaggta aacatcataa tgattgccag
     5821 tgtttgttaa atatccatac tcaagatctt catctgttga aaaggattca tttcccaagg
     5881 agattgatgc cacattacta ttaacaataa ataatgagca aatgctatct gaaagaacag
     5941 caagaaaatt cttcaccgct ttaatcctat ccgacgtgtc ggtatctaaa gcaagcaaat
     6001 tcttgttatc attaaatata aactcgggta aatgcagtat gcattttttg tcattcaagc
     6061 aactatcaat gaattgtttt ttaaaaaaca gaaagctcca tgacataaaa ctacccacac
     6121 cctgaatttt gttgtcagat tgcagatcaa cgccaaataa tgtctttaca tcttccctga
     6181 gattatagaa tgccgtatca acaagagcct cgatcgtttg tttatcaaca attttaataa
     6241 actcttcgta tacttgcttt tgcgtcttat ggctgcccag cgcccatgaa tactcatgtt
     6301 cttttaaaac cagtatggct cttaacagac aatatccatc gccatctact ggagcgtatt
     6361 ttaatccata tgttttatta atttcactct gaataaaaga tatgccagac aaatcaatac
     6421 cagtatctaa atcagatatt cccggcatat ttatctgttc tggctggata ctcatatttt
     6481 cattatctaa acaatactca gaaaaaacat catcacacgc attaatgttt tcaatatatt
     6541 ccataaagcc ataccaataa taacctttaa aatctgtgat agactctgaa cctctgcccg
     6601 gcaaggacac tctaacaata tcaacatctt gaacatattg tatagtatat gtaatacctc
     6661 ctgaaacccc tgaataaatt tgtgtatcat tagtacgcaa tgttctcata aataatgata
     6721 gatcattatc attctgcatt tcctcaaatt ctttttctag tgattcacat tccagacatg
     6781 gagtatcctc ttcatttact ttacgtctga gttcattgta caaccgcata aaacacacca
     6841 gtgtgttttc aaaaagcgtg attgaatatg tttgattttt ttcgtactca ttttttatat
     6901 cgttccaggc ctgtttcaaa taattatttg cagtgcaact aattccatgt atattactac
     6961 ccatatatag cgtctataaa atttaataaa taatgacgcc ctagttaaac ttaaagtgcc
     7021 tggttcaact atcaaaaatc gctcaccctt tttcacctgt ttaaaatatg ttcagcaacc
     7081 catcttgatg gcgacctcct ctccgcgatg atttcaataa catattctgt gttggcatat
     7141 gaaattttga ggattaccct acacttatag gagttacctt acaggggttc cttcaatttg
     7201 tgttgattta cgcgagataa cgctatggaa ttaaaggatt attacgccat catgggcgtg
     7261 aaaccgacgg acgatctcaa gacaatcaag accgcctatc gtcgactggc ccgtaaatac
     7321 catcctgatg tcagcaaaga accggatgcc gaagcccgct tcaaagaggt cgctgaagcc
     7381 tgggaagtgt taagtgatga acaacgtcgc gctgagtatg atcagatgtg gcaacatcgc
     7441 aacgatccgc aatttaaccg tcagttccat catggcgacg gtcagagttt taatgccgaa
     7501 gattttgacg atatcttctc gtcaattttc ggtcagcatg cccgccagag ccgtcaacgc
     7561 cccgccgcac gtggccacga tattgaaatc gaagtggcgg tattcctcga agaaacgctt
     7621 actgagcata agcgtaccat cagctataac ctgccggttt ataacgcctt tggcatgatc
     7681 gaacaggaaa tcccgaaaac gctgaatgtg aagatcccgg caggcgtcgg caatggtcaa
     7741 cgcatccgcc tgaaaggcca ggggacgccg ggcgaaaacg gcggtccaaa tggcgatttg
     7801 tggctggtga ttcatattgc gccacatccg ctgttcgata ttgtcggcca ggatctggaa
     7861 attgtggtgc cggttagccc gtgggaagcg gcgctgggta ctaaagtcac cgttccaaca
     7921 ctgaaagaaa gcattttgct gactatcccg ccaggcagcc aggccgggca acgattgcgc
     7981 gttaaaggca aaggtctggt gagcaaaaaa cagaccggcg atctgtatgc ggtactgaaa
     8041 atcgtgatgc cgccgaaacc ggatgaaaac actgccgcgc tgtggcagca actggcagac
     8101 gcccagtcgt cttttgatcc acgtaaagat tgggggaaag cataatggct aatgttacgg
     8161 tgacttttac tattaccgaa ttttgcctgc ataccggcat ctctgaagag gagttgaatg
     8221 aaattgtcgg tttgggggtg gttgaaccgc gtgagatcca ggaaacaacc tgggtatttg
     8281 acgaccatgc cgccattgtg gtgcaacgcg cggtacgcct gcgtcatgaa ctggctctgg
     8341 actggccggg gatcgcggtg gcgctgacgt taatggatga tattgcgcac ctgaagcagg
     8401 aaaaccgcct gctacgccag cggctttccc ggtttgtagc tcatccgtga tgtgtgtttg
     8461 ccggatgtga tgacgcatcc ggcaactgcg ccataatgcc tgatgcgacg ctaaacgcgt
     8521 cttttcaggc gcataaacga cgttatctgt tttggtggtc gcactccact aacaccagca
     8581 ataactggct tagtgccgcg taaaaaccaa agctgtcata ctgtcgacaa cgcgcagcaa
     8641 actctggtaa ccattgccgc agcgccgtca gtgttttttg ccgcaaactg tcgattcttc
     8701 gcgcaggaac ggtcccctct cccagcgaaa aatgcagata actcaacaac tcgagataga
     8761 tcgccagatg atctgccggt tcgttgaaat tgccactggt ttccatccct gcctcaacta
     8821 acaagcgttt aatctcttgc tcgtcctgtt tgtaggccga tgcatacggc agcgccgctt
     8881 gtttgtcggt catcagaaac aggccgcaaa agtcagcggc cagttccaga cgggcatcgt
     8941 cacgtactgt cagcgt
//
LOCUS       DAJTSI010000156         8783 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000156 DAJTSI010000000
VERSION     DAJTSI010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8783
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.156"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..671)
                     /gene="chbR"
                     /locus_tag="OO327_003409"
                     /pseudo
     CDS             complement(1..671)
                     /gene="chbR"
                     /locus_tag="OO327_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(679..1029)
                     /gene="chbA"
                     /locus_tag="OO327_003410"
     CDS             complement(679..1029)
                     /gene="chbA"
                     /locus_tag="OO327_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCR6019828.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(1088..2436)
                     /gene="chbC"
                     /locus_tag="OO327_003411"
                     /pseudo
     CDS             complement(1088..2436)
                     /gene="chbC"
                     /locus_tag="OO327_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2521..2841)
                     /gene="chbB"
                     /locus_tag="OO327_003412"
     CDS             complement(2521..2841)
                     /gene="chbB"
                     /locus_tag="OO327_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR6019829.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3140..3478)
                     /gene="osmE"
                     /locus_tag="OO327_003413"
     CDS             complement(3140..3478)
                     /gene="osmE"
                     /locus_tag="OO327_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR6019830.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3680..4507
                     /gene="nadE"
                     /locus_tag="OO327_003414"
     CDS             3680..4507
                     /gene="nadE"
                     /locus_tag="OO327_003414"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR6019831.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4737..5624
                     /gene="cho"
                     /locus_tag="OO327_003415"
     CDS             4737..5624
                     /gene="cho"
                     /locus_tag="OO327_003415"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR6019832.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5584..6159)
                     /gene="ves"
                     /locus_tag="OO327_003416"
     CDS             complement(5584..6159)
                     /gene="ves"
                     /locus_tag="OO327_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR6019833.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6362..6847)
                     /gene="spy"
                     /locus_tag="OO327_003417"
     CDS             complement(6362..6847)
                     /gene="spy"
                     /locus_tag="OO327_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR6019834.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(7177..8145)
                     /gene="astE"
                     /locus_tag="OO327_003418"
     CDS             complement(7177..8145)
                     /gene="astE"
                     /locus_tag="OO327_003418"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR6019835.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(8138..8783)
                     /locus_tag="OO327_003419"
                     /pseudo
     CDS             complement(8138..8783)
                     /locus_tag="OO327_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310478.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
BASE COUNT         2294 a         2238 c         2030 g         2221 t
ORIGIN      
        1 tcattaataa tctgcattgg tgttttgcca taatatcgtt gagtcgctcg cgtcaaatat
       61 tcctgtgatt tggctgacaa cgtcaccata ttctccagcg ccgattcact aaactgctct
      121 ttatcatgca tcttttctac cgtacttttc agccactacg gtacatcatc aatcacctgt
      181 tcttcgcggt aatgacgtaa acggttaatg acataaaaag taaccatctc aacaaactct
      241 tccagccctg tttcgcggaa atttaatgaa gaaatcactg tttccacata ggtgagaaaa
      301 gcgttattgg tccggtatac ctgcgaagca acaaagcaat aaggcaacaa cggcaggtaa
      361 tgctgctcaa aaaagcgttt actgatccca acgttcaata tgcgcgtggc accaaactca
      421 taaaaacttt gatggtgcga acctaacgga ataaaaacaa aatcgccccg ttccagtaac
      481 acgcgcttac cgttaatctc ctggaaataa cgcccggtta ataccagagt aaattcataa
      541 tagtcgtgct gatgcagtcc gctgatactc tcagttttgt tatagataaa cacatggaag
      601 tttttgccat taaacaactg ctgttctcgg gcagtggcaa tttccggcgc gttaatcact
      661 ggctgcatca tcgactcctt atgccttcag tttttcatga agctcaatta attcagtaat
      721 cagttcacgc gcaagcatgg acgtcattaa atgatcctga gcgtggacca gcaccagact
      781 cactttcatc ttaccttcgc ccgcatcgcc ttcaatcagt ttcgtctgta ccagatgcgc
      841 ttcattcaat gccatccgtg actgatccat catggctttc gctgcggcaa aatcgccctg
      901 cttcgcctgt ttcaatgcgg catacgccag gctacgcgct tgtccagagt tgatgataag
      961 ccccatcacc acttcttcca gctcttcagc ttctgtttgc gtatcaggaa tattatcgag
     1021 atccatcata catcgttcct ctctcttctt accggcaaga ttacccgtac cggcatcgat
     1081 taaaatttca ggggttagcg atatcttctt cgctctcttc tttatcaatc gcattttgtg
     1141 ctttgttagc caccacaaca aagggcagat aaattaacgt tgcgatgcca aggttgaaga
     1201 gtgcgaccag caatgcggcg acgctaccgt tggtgttaaa gaaggctccc agaccggttg
     1261 gcatggtcca cggtgcaata ttggtcaccg gaggaatgat gcccatgtag tacgctgcga
     1321 gggtgattgc cgccagaatc ggttgtacca gtacaaacgg gataaacatc accgggttca
     1381 tgataattgg cagaccaaac agaatcggtt cgttaatctg gaagatgccg gacggcagcg
     1441 ccagttttgc cacctgacga taatcagcac ggcgagaggc gataaagata gccaggatca
     1501 ggcctaaagt cgcaccactg cccccaagga aaataaagga gtccagcatc ggcttggccc
     1561 agatatggaa ggtcttaccg gctgccagcg ccgcttcgac ggaaccatat tgctgatagg
     1621 tcgcgatatt ttccagcgcc cacggcgtca taatgccgtt gtccagtgcg gtcagcgcca
     1681 gcgcgccatg aataccgaag aaccagagca gtggaacaaa gatcacatag gcccagccca
     1741 ccacgctacc caacgatgcc agtggggttg agatggtatc cataatgatc tgatggaagt
     1801 tggtgcccca ggtattcaac gcccaggcaa taatccccat cacggaaaga ataataaagc
     1861 cgggaattaa tgccgagaaa gaacgcgata ctgaagcagg tacgctgtcg ggcagtttaa
     1921 tgacccaatt gcggcggaca ataaaggtaa acatttctgc caccaccagg ccaataataa
     1981 tcccggagat gatattcgcc ccacctaacc agtttgcgcc aaccgcatag gcctcaccga
     2041 cactatatgg ggtgacggtc ataaatgctg caacggataa caacccagcc gccagcgcat
     2101 cgactttacg ctcttctgcc agcgccatgc caataaagaa cggtgccatt aaagacatta
     2161 ttcctaatgt tccgttatat acgttgccgc caataccttt cagaccatta agtgtttcaa
     2221 tggttgaggc gtcgaggcga atacctaagg aataaaaaac gacccctccc caaagcttag
     2281 aaaaacgttg ttaattaata caaacatggc ccccgcaagg gttaacggca ttaagcgaat
     2341 aaagccattt ttgattgcat taacgtgtgg ctgctttcct attttaactg caaaagggag
     2401 gagtaccttt tcaagcgatg caataacatt actcatagaa aaataccctt aaaaaccgca
     2461 atttaaatat tacggtattg atttatgaaa taactctttg acgggaaaat ttaaaataaa
     2521 ttaatttgct gcggcttttt taatcgctgc aaccgcagcc ttaagcacgc ctaaaccatc
     2581 gactttgcca taaagcagcg agtcaattac ttcaaccggt ttgttgggta acaaacgctg
     2641 gatttcgggc aacatataag caatctgcgg ccctaataac acgacatcgg cattctgacc
     2701 tttttcacca gccagtgttt ccggaaatgc ttcaataatg accggaactt catatttttc
     2761 tgcctgtgcg cgcatttttg ataccagtaa agagatagac atgcccgcag aacaaaacag
     2821 ataaatgtgt ttcttttcca taaaactgcc ctcatcgacg attatctgtc agccagacac
     2881 tccgcaagcc ttgacctgct tccatgctct gggtaacttg cgaaaccaac atgacttttt
     2941 ttgtggctga aaggagtata cggtatagac ctgagcaata gtatttcttt gaccttataa
     3001 attgtttctc cttgacctgt ggttatgacc ctcttcacat ttcgggcaaa taattcgcga
     3061 agataattaa gaactcaggc caaaaaaaac cggcgcaatg gccggtttcc gttgttactc
     3121 aagctttcag acgaattgat tacttcgcag cctgtggatc agtgtcgtat tcagcacagg
     3181 tctgataacc ggagttgatg acatgtccgg tatcatctaa cgcgacaaag taggtttctg
     3241 ctttaccatc acgttgaccc aggatgtagg tctggcaagt accgcgagca tggatcatgc
     3301 tcacttcaga cgaaggttta cccgcaattt gtgcaacctg cgcccggctc atgccttttt
     3361 tgacgtcttt caccacaggc tgtacaaact ggtctttggt acgatcataa gctgtacaac
     3421 ccgccagcat ggttaatacc gccgctgcac tcagaattcc tgccatattc ttgttcatat
     3481 tccgtcctct tgtttatcag cgtgttagat aagcctggaa tacattgggc gctttttcaa
     3541 gcccgtgaac gaaacggctc cgctttcaga ggattcctgt atgacgtttt aaccaccatt
     3601 cagcccgctg tcgcttgtcg tttcagtagc aacgggttag ctttaaggaa gttttgtctt
     3661 ttctgtctgg aggggttcaa tgacattgca acaacaaata ataaaggcgc tgggcgcaaa
     3721 accgcagatt aatgctgaag aggaaattcg tcgtagtgtc gattttctga aaagctacct
     3781 gcaaacttat ccgttcatta aatcactggt gctcgggatc agcggcggtc aggactccac
     3841 gcttgccgga aagctgtgcc agatggcgat taatgagctg cgccaggaaa ctcgcaacga
     3901 atcactgcaa tttattgccg tacgcctgcc ctatggtgtt caggctgacg aacaagattg
     3961 ccaggatgcc attgccttta ttcaaccgga tcgcgtatta accgttaata tcaagggcgc
     4021 ggtattggct agcgagcagg cattgcggga agcaggcatt gaactgagcg attttgtccg
     4081 tggcaatgaa aaagcgcgtg agcggatgaa agcacaatat agcattgcgg gtatgaccag
     4141 cggtgtcgtg gtgggcaccg atcatgcagc agaagccatt accggattct tcactaaata
     4201 tggtgacggc ggtacggata ttaatccgct gtatcgtctc aacaaacgcc agggtaaaca
     4261 gttactgacg gcattaggtt gcccggaaca cctttataag aaagccccaa ctgccgatct
     4321 tgaagatgat cgcccttctc tgccagatga agtggcactc ggcgtgacct atgacaatat
     4381 cgacgactat ctggaaggga aaaacctacc tgaacaggtc gccagaacaa tagagaactg
     4441 gtatctgaaa accgaacata aacgccgtcc gccaattaac gttttcgatg atttctggaa
     4501 aaagtaataa tttgcaccta ttggattgtg cgaaaaagag taatttgttc acgccggatg
     4561 cggcgtgaac gccttattct acctacaaaa ctatgcaaat tcaatacatt gcaggagtcg
     4621 aataggcctg acaggcgtag cacgtcagac ggtgtaacct ttgtcatcaa cccgcctctt
     4681 ttttaatcgc ctcccgcctg ttacactgga tagataacca gcattcggag tcaacagtgg
     4741 tacggcgttt aacttctccg cggctcgaat ttgaagctgc ggcaatttat gaatatcccg
     4801 aacatttaca ttcattcctt aatgacttac ccacccgacc aggggtgtat ctgtttcatg
     4861 gcgaaagtga caccatgccg ctctatatcg gcaaaagcgt taatatccgc agtcgggtgc
     4921 tttctcattt acgcaccccg gatgaagccg ccatgctacg gcaatcccgg cgaattagct
     4981 ggatctgcac cgcaggcgaa atcggcgctc tgctccttga agcgcgatta atcaaagaac
     5041 aacagccgct gtttaataaa cggttgcgcc gcaatcgcca gctctgtgcc ctgcaattaa
     5101 atgaaaagcg cgtcgatgtg gtgtatgcca aagaggtgga tttttcacga gcccccaacc
     5161 tgtttggcct gtttgccaat aggcgcgcag ctttgcaagc attgcagagc atcgctgatg
     5221 aacaaaaact ttgttatggc ctgttgggac tggaaccgtt aagtcgcggt cgtgcatgtt
     5281 ttcgttcagc gctaaaacgt tgcgccggag catgctgcgg taaagagagc catgaggaac
     5341 atgcgctacg cttgcgccag tctctggagc gtttgcgagt ggtgtgttgg ccttggcaag
     5401 gtgcggtggc actgaaagaa cagcacccgg aaatgactca atatcatatt attcaaaact
     5461 ggctgtggct gggggcggtt aattcgctgg aagaagcgac aacgttaatt cggacacccg
     5521 ccgggtttga tcacgacggt tataaaattc tttgtaagcc gctgctttcc ggtaactatg
     5581 aaattactga acttgatccg gcgaatgacc agcgagccag ttgatttcac tgaacaacag
     5641 tttaccttgc ggttgtaaaa gccgcagggt gtgcctgcca tcgaaccagc acacgccctg
     5701 gtcggtagtg agcagtttgt cgcccagttg ccacgccccg ttaatcacaa acaccacccc
     5761 gccgcgtgag ccaaaggtag taaacgtacg ttcggcaatt ctgactttcg ccttacagac
     5821 atccaggcgc gtcataatat tgaaatccat cgacatctgc cctgccgtca gtttcgcttt
     5881 taccacctgg tccgccgtaa aggcaaaagg ctgtaacggt tttaaggtat ggttaaagcg
     5941 gtctgcgctt tcaaggagca tctcgccgcc ttccagcaac gtcactatcc tttccatgcc
     6001 gggaaacaaa gaaaactccc cattcgccgc gatggatgca atgctggcac gccagtaaaa
     6061 atcacgtttt gccggtggga aagtgcaaat ttcgcgcgtt tcaccggcag cgtttcgcca
     6121 cagattcacc gacattttac gcatatcaaa gtattccata ctcgctcccg gcccgttctt
     6181 ttcattgtta tcgttcaggt aaccgtggat ggttatcggc aactcgggtg atttacgtta
     6241 gtggtgatca ggcttttttt acaactgttt gagaagagaa aagaaaaccg ccgatcctgt
     6301 ccaccgcatt actgcaaggt agtggacaag accggcggtc ttaagttttt tggctgaaag
     6361 attattcagc agttgcaggc attttacctt ttgccgctgg acgttctgtc agacgcttct
     6421 caaaattagc attaaattgc tttttctgtt ccggcgtcag gatgttgtaa attttgttct
     6481 gggtttccat gtgcgccagc atgttagctt tgcgctgttc ttccattttt gcgatctgcg
     6541 cttcagcttt tgctttatcg aaggtatcgc tggcaatgat gtcatgcatt gcgcggcgtt
     6601 cttccagcgg cggacgtttc atctggtcac gctggccttt catgatttcg tggatctgct
     6661 gtttctgcgc gtcggtcagg ttcaggtctt tgaacatcat gtcctgatgc ggaccgaact
     6721 tgcctttgtg gtgcatcatc ggcttcgcgt cagccggtgc tgcggtagtg gtgtctgcgg
     6781 catgggccag gttagccgcg ccaagagcca gggtagaggc aacaaacagt gcagttaatt
     6841 tacgcatatt ctatatcctt cctttcagtt atttattacg gctttcttaa gtagcgtgcc
     6901 gtgttgacga gattaacttt actgacttta gcgtcaatta atcagagcaa cggtaaaaca
     6961 atgaaagtgt aaaaaacact tttgcgccaa ttatggagaa aaaaagaaaa tttgatggag
     7021 agtgatgaga gaatattaca acacgatgat tttgcagaga ttatgaagaa ctataccgga
     7081 tgactggtga taaataaagc aaataaccag gattaatctg tattaattta taagaaagca
     7141 acttaatacc cgcagaatga tttctgcggg taagtattag cttatttttt cgagcattaa
     7201 tcccgcgcgt aatcccaacg ctaccaacgg attagggaat aacacatact ctacatcatg
     7261 ggttacggta aaacgttcct ctccgtcctg cgccagcaat gttcctttct caaacggcat
     7321 aaaattcagc gtgtcacttg ccatatgcat ttcgaaggac ggcgagtggc gagtaatttg
     7381 cgaaaccacc cgataacgga gcggcggtgt tctcacgata ccgacactct caccagatag
     7441 cagcgcagca attgcgctgg cagttactgc aaactggcga agatcgtttt gcccaaaggg
     7501 caacgctttg ccaagttcca acgtacaggc cagcgcgcca aaatgtctga cgctgaaatg
     7561 ggtaaacgta ccaccaggct cctgatggaa caccagcgcc tccagccccg ccgcacccag
     7621 ccacgtcaga aatttctcgt cccagggaat gtcgcgttgc ggtaatacac cgaactgcgg
     7681 atgcaaggag ccacgaattg cggtatgtag atcaaggtgc caacgcacag attctttgcc
     7741 ctggtcataa aaatcttcca ggcactgttc cagttcgcgc gcccgacagg tttctccgct
     7801 ttcagcaaat agctgccaac gaccgccaaa cattcggttc atatcgctat ggcaataacg
     7861 tttcccttgc ttcagcgcag gaggattccc gaggatcacc agcaaccgcc aacgtaacgg
     7921 gatctcgccg tgagatatcg cgccaagcaa cgcgtccagc atttccacag gtgccgtctc
     7981 attaccgtgg atccccgcag aaatcaccag cgcgccttgc ggtggcgtta atggcgtcag
     8041 ttccagcaca ccatcgccca gccagcgcca gcgaacgccg ttgatttctc gctcggtgat
     8101 aaccggtttt ttacccgtta aggtcagagc aagaaaatta tccattgccc cctccctcgc
     8161 gctggaacgg ataaaccgaa ccgagattca gtaattggct caatacatcc agtgcttccc
     8221 gcccttcgcg cagcaattgc gggtcggcca gatcggcagc agtaagacga tcgcggtagt
     8281 aacgatccac ccagtcattg agcgcattaa acaaggtatc gttcatcatc accgccggat
     8341 tcaccgcccg gcgttcttct tctgtcaata ccacccgcaa ccgcaggcac gccgggccgc
     8401 cgccattcgc catgctttca cgtaagtcaa agacttttag ttcgctaatc gggttgtcag
     8461 cggcaaggag ttcattgaga taaccccata ctccggcgtg ttcccgacac tcctgaggca
     8521 gcaccaacat catggaacca tcatcgcggc tcaacagttg gctgttaaac agataggtag
     8581 acaccgcatc cgacacggaa acctgagttg ccggaacttc tatcgccata aaaccgttca
     8641 cccgcgtgcc agtaactgtg actggcgagc gaacgcctgt tggtggcaaa acagcacctg
     8701 gcggttactc acggcaatca cgtcattatg aaaaacgctc tggtcgataa cgtccgggtt
     8761 ttgctgggcg aaaatcacct gtt
//
LOCUS       DAJTSI010000157         8760 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000157 DAJTSI010000000
VERSION     DAJTSI010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8760
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.157"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            8..439
                     /locus_tag="OO327_003420"
     CDS             8..439
                     /locus_tag="OO327_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR6019836.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(586..2748)
                     /gene="uvrD"
                     /locus_tag="OO327_003421"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(586..2748)
                     /gene="uvrD"
                     /locus_tag="OO327_003421"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR6019837.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2832..3548)
                     /gene="yigB"
                     /locus_tag="OO327_003422"
     CDS             complement(2832..3548)
                     /gene="yigB"
                     /locus_tag="OO327_003422"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR6019838.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3548..4444)
                     /gene="xerC"
                     /locus_tag="OO327_003423"
     CDS             complement(3548..4444)
                     /gene="xerC"
                     /locus_tag="OO327_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR6019839.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4441..5148)
                     /locus_tag="OO327_003424"
     CDS             complement(4441..5148)
                     /locus_tag="OO327_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR6019840.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5145..5969)
                     /gene="dapF"
                     /locus_tag="OO327_003425"
     CDS             complement(5145..5969)
                     /gene="dapF"
                     /locus_tag="OO327_003425"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR6019841.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6006..6209)
                     /locus_tag="OO327_003426"
     CDS             complement(6006..6209)
                     /locus_tag="OO327_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6019842.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6398..7876
                     /locus_tag="OO327_003427"
                     /pseudo
     CDS             6398..7876
                     /locus_tag="OO327_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7873..>8760
                     /locus_tag="OO327_003428"
     CDS             7873..>8760
                     /locus_tag="OO327_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR6019843.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEP"
BASE COUNT         2032 a         2388 c         2251 g         2089 t
ORIGIN      
        1 gcagaaggtg gcgttaaatc tcgcttcagg tgaagggaat ttctttatcc gtcctggcgg
       61 cgtgttttat gtcgcgggag ataaagtcgg catcgttcgt ctggatgctt ttaaagccag
      121 taaagatatt cagtttgcgg tgcagtcagg accaatgttg atggaaaacg gtgtaattaa
      181 tccgcgtatt catcccaacg tcgcctcacg taaaattcgt aacggtgttg ggattaataa
      241 acatgggaat gccgtgtttt tgttgagcca gcaggcgaca aatttttatg attttgcctg
      301 ttatgccaaa gcgaaactga acgttgagca gctgctttat cttggtggca cgatttcaca
      361 tatgtatatg aaaggcgggg caatcccgtg gcaacgctat ccttttgtca ccatgatttc
      421 cgtcgagcgg aaaggttaga atgatagctt tgtgtcagat actaaatatg ctgctgatgc
      481 cggatgcggc gtaaacgcct tatccggcct acatgacgtt gcaatttatt gaatctgaat
      541 gattttttag gccaaataag gtgcgcagca ccgcatccgg caacgttaca ccgactccag
      601 ccgggcgtaa gctgccacca gccatttgat tccctggccc tggaatgcca cctgcaaacg
      661 gctatgttca ccgctgcctt ccatattgac gatggtgcct tcaccaaact tagcgtggcg
      721 tacacgctgg ccgagcttgt agccgctgtc gttctcgacc atcggcgtac ccatccgctg
      781 atggctgacc gggcggctta ccgtggcgcg caggcgcacc tcttccacac actcttccgg
      841 caactcgcca ataaagcgcg acgggcgatg gtaaacctct ttgccataca gacggcgagt
      901 ttccgcgtag gtcagcgtca gtttctgcat cgcgcgggtt acgccaacgt aggccagacg
      961 gcgttcttct tccagacgcc cgccttcatc cagcgacatc tggcttggga acatgccctc
     1021 ttccataccg acgataaaca cctgcgggaa ctccagacct ttcgccgagt gcatggtcat
     1081 cagttgtacc gcatcctgcc aggtatccgc ctgcccttcg cctgcttcca gcgccgcatg
     1141 ggataggaac gcctgcagcg gcattaaatc ttcgtcttct tcgttgtagc tgaactggcg
     1201 cgttgccgtc accagttcct ctaagttttc gatacgcgtc tgaccttttt cgcccttctc
     1261 ctgctcgtac atggtacgca ggccggagtc tttaattacc cggtcagtct gtacatgcag
     1321 cggcatatcg gcagtttcct gcgctaaggc gtcgatcagt tccataaacc gctgtaaggc
     1381 gctggcagca cgtccggcga gggctttttc ctgcaacagt tcacgacatg cctgccagag
     1441 tgttaactgg cgatcgcgcg atgtctgacg taccacgtcc agcgtccggt caccaatacc
     1501 ccgcgttggc gtattcacca cacgctcaaa ggccgcgtcg tcgttacggt tggcaatcag
     1561 gcgcagatac gagagcgcat ctttgatttc ctggcgttcg aagaagcgca tcccgccgta
     1621 aatacggtac ggcatactcg cctgcaacag tgcttcttcc agcacacgcg actgggcgtt
     1681 actgcggtag agaatggcgc actcggcaag cgccccgccg ttgtcctgcc aggttttgat
     1741 gcggttaacc acaaaacgcg cttcatcgag ttcgttaaaa gcgcaataga gggaaatagg
     1801 ctcaccgtcc gcgccatcgg tccacagttt tttacccaga cgcccgttat tgttttcaat
     1861 cagggcgtta gcggcgctca gaatattgct ggtagagcgg tagttttgct ccagacgaat
     1921 aatttcggca ccggggaaat cattaaggaa acgctgaata ttctccacct gcgccccgcg
     1981 ccagccgtag attgactggt cgtcatcacc gacgatcatc actttgccgg tgtcgcccgc
     2041 cagcaggcgg atccacgcgt actgaatgtt gttggtatcc tggaattcgt ccaccaggat
     2101 attggtaaaa cgttcgcggt agtgttgtag gatatgcggc ttgttaagcc acagttcgtg
     2161 agcgcgcagc agcagctcgg cgaagtccac caggcccgcg cgatcgcacg cttcctgata
     2221 cgcctgatac accttctgcc aggtctgctc caccggatta ccgtagcttt gaatatgatg
     2281 cggacgcagg ccttcatctt tctggctgtt gatgtaccac attgcctggc gcggcggcca
     2341 ctgcttctcg tcgaggttca tggctttgat cagacgctta agcaggcgta gctggtcttc
     2401 gctatcgagg atctggaaat cctgcggcag attggcgtcc atatggtgcg cacgcagcag
     2461 gcggtgcgcc agcccgtgga aggtgcctac ccacatgccg ccctggctgg tgcccatcag
     2521 ttgtccgata cgatgacgca tctccgccgc cgctttgttg gtaaacgtca cagccataat
     2581 cgagtatggc gagcagtttt ccacgctcat cagccaggcg atacgatgca ccagtacgcg
     2641 cgtcttacca ctgcccgcgc ccgccagcac cagaaggttg ctgcgtggcg cggccaccgc
     2701 ttcgcgttgt ttgtcattaa ggctgtcgag caggtaagaa acgtccattg gcaccgccgc
     2761 gtaaaaatag gttgggcgga gaagcgcaac gccctccgcc aaaaagctgg gtatatatac
     2821 agatttgctg attatatcag cgaggtcaga gatgccaacc gggaaatttc cagatgtggc
     2881 agtaaacggc tgtcccaggt ttgcatcaga tcgccatttt ccggtctgat ccaacaagcc
     2941 tgcattccgc tgcgaattgc cccacccacg tcagtggtga gatcgtcccc aacatgtaag
     3001 atctcgccga tcggcacgtt gagtttttcc gcagccaaaa agtacatatc gctgaacggt
     3061 tttgagcgcc cgtgcgggcc agcgcgcagc acaaactcaa aataatcccc caaaccaaac
     3121 agttccggct gggcgttacc gttggtgatc gccaccagcg gccatttctt cgccagctgt
     3181 tttaaggtgt cgtgagtttg ctgcgggacg tcgattcggc tgcgccattt ggcaaagtta
     3241 atcattgctg cgtgtgcgcc tgcactggct tcttcggcac tcagcccggc gtcgagcatc
     3301 gcttgttcaa tcgaacgaaa acgccaacgt gtcacgtcgt gataaatctc gggttccgct
     3361 tcccgtaccg cctggcgcag gcgttgcaga tcttcattct ggaagctgcg cagcgccgga
     3421 tgataatttt gcacaaaggt aagcgcctct cgctcggtgc gcaaaatcac cggacggtta
     3481 tcgtaaaggg tatcatccag gtcaaaggtg agcgccgaga tgcgccccaa aggccggtaa
     3541 aaacgcatta tttcccccgt ttggcgcgtg gatgcgccgc atcgtacacc gaggcaaggt
     3601 gttgaaaatc aagatgagta tagatttgcg tggtggagag gttggcatga cccagtagct
     3661 cctgcacacc acgaagatcg ccgctcgact ccagcatatg cgtagcgaac gagtgacgta
     3721 atttatgtgg gtgaacgtga ttattcagcc cttgttttat gccccattcg gcaaagcgtt
     3781 tctgcacatt acgcgcggag atacgcttgc ccagtttcga cagaaaaagc gcgtcgtctt
     3841 cgctaccaaa caggtcgcgc aaatcaagcc agtgctcaat ccacgccaca gcgttgcgac
     3901 caatcggcag gcggcgctct ttgctgcctt tccccatcac ccacacttcg ccggactcca
     3961 ggtcgaggtg tttgatatcc agccccacca gctcggaaag acgcagaccc gcgccgtaca
     4021 tcacttccag cattgcacgg tcgcgtacag cgaggggatc gttgatatca atatccagca
     4081 gccgattcat atcgtcgacg tcgatatttt tcggcagatg acgcggcgct ttcggtgccg
     4141 aaactccttt cgccgggtta gctttgagtt cgttctggct gaccagccag tcaaaaaagc
     4201 tacgtagcgc agaaagccgt aacgccagac ttgctgctcc cagcccttta cggcgactgc
     4261 gtacagcaaa attgcgtacc atcgctgcat cgcattgttg ccagctttgc aggccgtttt
     4321 cgctggcaaa attgatgatc gcctcaagct gacgctggta gttaagtagg gttatcgggc
     4381 taagctgacg ctccacgctc agataacgta ggtagcgttc tacatcggtg tgtaaatcgg
     4441 tcatacgcgt tcaatccaac gctccagaag ctccggcaac atcagcgcaa tttcatgaag
     4501 taactgcgtt ccctgccctt gttgatagtg actggcatcg cgactggtaa acagcacaac
     4561 acccaaatca gcatcgcttc ccagcatcga catcgccacc gatcccaccg ctttcgcttc
     4621 cggtagcacc accagcagct ccggtccgtt aagcggccca agatagtgct gttcctgccc
     4681 caaacgctga atacgcagcg gttcgaaaga ctgacggctt aatgccagat gagtgtggtt
     4741 cgacggcgca cctaagcgcc agcgatctgg aaacaggcgc agactcgcac cagctaggcc
     4801 gagatcgcgc gcccagcggt gaaagcgcat cagcatatcg tcgagactgc tggcggcggt
     4861 gagactgcgc tgcaggtaga gtaggcgata aaacagccct tcgttggcga tagcctgctc
     4921 catcaacaac gccatgttct cttccagaac atgaatgtga ttacgtgcgc gggccatgtg
     4981 ccactcgacc aacgaaacgg tgccgcgtac cggatgcggc acacgtatcg cttctactgc
     5041 gcgcgcattg cggataaaaa actcaggatt tttaatcaga taatcgacaa ccgcccggtc
     5101 atcaagctcc gtgagtgttt cctgcagttc ttcccctggt tgcttcatag atgaataaat
     5161 ccatcgtaga catgtaccgc cgggccagtc atatataacg ggtgacccgg acctttccag
     5221 gcgatatcaa gacgaccgcc ggggagttcc acgcgtactt cttcgtccag caaaccttgc
     5281 tgaatcccta ccgccaccgc cgcacacgcg ccgctgccgc aggcctgggt ttctcctgcc
     5341 ccacgctcat aaacgcgtaa acgaatatgc tcgcgcttaa ccacttgcat aaaaccgata
     5401 ttggcgcgct ccggaaaacg ctcgtgactt tccagaacag gaccaagcgt ttctaccgcc
     5461 gcggtatcga catcatcgac ctgaatcacg caatgcggat ttcccatcga caccacgccg
     5521 cataagattg tctgctcggc ggcgcgcata atataggtct tttccgcttt gttagcgcga
     5581 aacggcacgg cggaaggttc gaagttgggt tcgcccatat ttacgcggac cagatcatca
     5641 tcggtgacgg tcagaaccat ccgcccgttg gcggtgctga cgcggatatc acgcttattg
     5701 gtcagtcctt tcagacgcac aaaacgggca aagcagcgcg caccgttacc gcactgcgcc
     5761 acttcactgc catcagcatt gaaaatgcga tagtgaaaat ccagttcagg atcatacggc
     5821 ggctcaacca ccagcagctg gtcaaaccct acccccaggt gccgatccgc caggcgacga
     5881 atcagctccg gtgaaaaaaa gacattctgc gttaccgcgt cgacgaccat aaaatcgttg
     5941 ccaaggccat gcattttcga gaactgcatc atttactcca atcacgcggg tacagaaact
     6001 gacttttagt aattcacctg ggatggacca tcgccagtgg cgcgatcgtt tttatccggc
     6061 accgtggatt gcgtttgcgt ctctaccggt ttggtcggcg gcggtgcgtt tttatctgca
     6121 ggcgggaaat agagcggacc tttcagaccg cagcccgtca ggctgaagag agtaagtaat
     6181 acagtgagtg ccttaaacac gtttttcatt atggattgcc tgtaaagttc atcgctttct
     6241 gctttctatc atcgcaggag aaggcgtaaa agcaagcgtt tagcgactct ttatgaagtc
     6301 tgaaaagtga ggaaatgctg cgagtataac gcttactcgt gaggatttat gttctgctgg
     6361 cagtttcgtg atcaacacct cgcgccagga tggctttatg cactatcgca atgtggtttg
     6421 tttgttgtct tgttcattgt ttttatcgtc ggcatgggga tgtcggcttt ccgcacctga
     6481 acataatatt taccagaaac agggaaaagg cgtggtgtac ctgcgccctt ataaagaaac
     6541 aaatctttct ctacctgacg tgaattacaa gagtttgcgt cggttaccca atttattaat
     6601 agacccgaca acactagacg aatgggataa agaaccgcct ctgactgatt tgacaaccga
     6661 ctacctctat gagggcgcac aggcctggta tcctcattat tcatggcata gcgatggtcg
     6721 caatatcctc tacgcgggtg aagttgtgca aaaccctccc ggcacacctc ccgtcgatgt
     6781 cgcctcgttc aaggcatggg gtgattttgc cgcagataaa cacagtctct attttgaagg
     6841 caagcgcacc gatgacaatg gtggtggaaa tagcttggat atcaaaacgc tgcatcaggt
     6901 ggaattccgg cctccctggg atcctgactt actggggctg atattacgtg acgctaattt
     6961 cttgtatatc aatgggcatc gcctcgctga ccccgagagt ttccgtgtgc tggcgcagaa
     7021 gtcctgggat cagcgaggaa agttttccac cacgtttaac ccctgcattg ccgtaccatt
     7081 tggcccctgg gataccctgg ctcgtacacg gaccaaaatc ctgctcaacg gcgaacagct
     7141 tgatgccgac ccggacacct tttccgtcgt gcgctggatg cccggctcac tcttgacctg
     7201 gcgtgataaa aacgggctgc agcgtaaagt cctcgacaag gaaaatctgg cgtgggatga
     7261 agatttaaca aagcactgtc tggatttttc tctgctggaa aagaaagtgt tctggcgtaa
     7321 aggacctgct tgtaaacagg aagaattacc cggactcgat ccggagcagt ttcaacccat
     7381 cagtgatgct gtcgcccagc atcaggactc gctttatacc atcatcgaaa cagagtctgg
     7441 taaccgtaag ctggagatcg tgaaacttga tgatcccaat cttattatca acaaacgttt
     7501 caacgccggg aaacgccacg gctatttact tacgcgtgcc gaagggtagc cataccattc
     7561 cggtttacac gtgtttgaat ctgacggacc gctgatctta ctggataacc gttctccgga
     7621 tgaacgcgaa gcccatctta atgaccatcc ctttttgcgc agatggtatg cccgcgataa
     7681 ccgctacatt tacagctttg atggcgcgca gctctggcga taccgcaccg ctgatccgaa
     7741 acaagttcgc ttaatctgga aggaacaaca ttcgggatat gtctatggcg taaattacaa
     7801 aacgggatat ctggacggaa aaattactga tgacggcgaa tttattcctg ccccgcgcaa
     7861 tgaggcgaca aaatgaaggg tcaaataatc acagttgcac tcatcctgtt gggacacaca
     7921 tttctgacgc ctgctgtaca ggccataggg ttcgactatt acaatgatca cggtgttatg
     7981 tcttacggta aaggttattg gggggataag aaaatcattg cccagtttcc ggagatgagc
     8041 agggccggtt tgcgccttgt gaaaaatatc tctgggaaaa cgggttatat accttccgat
     8101 gaaataagca tagaaaatga ggaataccac tgggtgacag acggccgcgt tattctctgg
     8161 cgtggcaaaa tcgttagcaa tccaccggga acactcactg tcgatattgc cagctttcag
     8221 gctatgggcc gcttcgcggt cgataaatat agcctctatt ttgacggaca gcgcaccgaa
     8281 agtaatagcg gcgcgtcacg tgttgagctg gcgacactaa aagctatcga aggtaactct
     8341 accacgctgg tggatagtaa aaatctctac ttgtccggtc gacgtcaggg gagcagcagt
     8401 gatgttactg tattagaaaa aagatggtgg ggtattaatc cacgtcttat gagtgtaaac
     8461 agaaattcat attccaatga tctgcttatc cgcagtgggc agaatattta tttaaatggc
     8521 gttcacctta cggcgaatgc agactcattt gagataattc gctggatacc tcattcactg
     8581 ctggtttttc gcgacaataa gggtctgcat cgttatccct ttggtcaatt atcaggcaaa
     8641 gcgatacccg tagatgatga cgtctctttt gaagtagggg aaagtcgcgt tcgctggcgt
     8701 aaacagctca cgcccgaccg tcagtggagc aagtggatag acctaccagg tattgaacct
//
LOCUS       DAJTSI010000158         8684 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000158 DAJTSI010000000
VERSION     DAJTSI010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8684
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.158"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(34..360)
                     /locus_tag="OO327_003429"
                     /pseudo
     CDS             complement(34..360)
                     /locus_tag="OO327_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(370..918)
                     /locus_tag="OO327_003430"
     CDS             complement(370..918)
                     /locus_tag="OO327_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR6019844.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(918..1625)
                     /locus_tag="OO327_003431"
     CDS             complement(918..1625)
                     /locus_tag="OO327_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6019845.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(1640..2317)
                     /gene="ydjY"
                     /locus_tag="OO327_003432"
     CDS             complement(1640..2317)
                     /gene="ydjY"
                     /locus_tag="OO327_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCR6019846.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(2322..3032)
                     /locus_tag="OO327_003433"
     CDS             complement(2322..3032)
                     /locus_tag="OO327_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6019847.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(3199..4005)
                     /gene="xthA"
                     /locus_tag="OO327_003434"
     CDS             complement(3199..4005)
                     /gene="xthA"
                     /locus_tag="OO327_003434"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR6019848.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            4451..5029
                     /locus_tag="OO327_003435"
     CDS             4451..5029
                     /locus_tag="OO327_003435"
                     /inference="COORDINATES: protein motif:HMM:NF012428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6019849.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDATCAVIVEPI"
     gene            5045..5671
                     /locus_tag="OO327_003436"
     CDS             5045..5671
                     /locus_tag="OO327_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6019850.1"
                     /translation="MPASNAFLQGLRELCDRHNALLIFDEVQTGVGRTGELYAYMHYG
                     VTPDLLTTAKALGGGFPVGALLATEECARVMTVGTHGTTYGGNPLASAVAGKVLELIN
                     TPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRGLGLLIGCVLNADYAGQAKQISLEA
                     AKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDRFAAACEHFVSRGSS"
     gene            5668..6702
                     /gene="astA"
                     /locus_tag="OO327_003437"
     CDS             5668..6702
                     /gene="astA"
                     /locus_tag="OO327_003437"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR6019851.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            6699..8177
                     /gene="astD"
                     /locus_tag="OO327_003438"
     CDS             6699..8177
                     /gene="astD"
                     /locus_tag="OO327_003438"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCR6019852.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            8174..>8684
                     /locus_tag="OO327_003439"
     CDS             8174..>8684
                     /locus_tag="OO327_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HCR6019853.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFKDEEKFS
                     VHSALPQVAL"
BASE COUNT         2025 a         2230 c         2391 g         2038 t
ORIGIN      
        1 aatgccagtc agttgcttaa ctgactggca ttactctttt aaggtgcgga agttttcgcc
       61 gttcacccag agcagatcca ccgagccgcc cgttttacga ccggcagcag cttcggtctg
      121 aatgcgcttc accgcgtctg cggcatccgc gagacgaaca atcttcaggt ttatagcgta
      181 atgggttttc atctcgccgc taacccagtc gagatagcgg ttaattgcgg tatcgccgcc
      241 ccaggcgtta aaccagacgg tttgcccttt ggcctcattt ttaatttcct gccagtcact
      301 ggcatgtgtt gccaaagaaa acagcgataa caatcccagc aaccacccac aatggcgcat
      361 cttccctcct taagaacgtt tttccggaat acggcacagc ccctgagcgg gaatgtccat
      421 ggcagaatta aaacgagccg acaggttttg caggccgatc aatgccgtca gttcggtgag
      481 cgcctgagcg tcaaaatgcg cagccagtcg ggtacgcagg gcatcatcga ctgttggcgg
      541 tgtgacgctt gcggcttcgg cgtactccag cgccagccgt tcttcatcgc taaagagcgg
      601 gctttgccgc cagtcagcca ctgccagcag tttatcggtg ctgccggtac gctcggcgac
      661 tttcatactg gtgatatcca cgcaaaactc acacaggcac atttgcgcaa tgcgcgcgct
      721 gacaagcgat cgcactaccg gatcgagcgg tgagcgtttg cgctccagcc acccaacaaa
      781 cattgacacc aaataaaaga taaacgggat ccgcccccac cagcgaatcg ggcttagcac
      841 ctcgccgtaa tggcgacgat gcagccacgc ctgcggacgt aaaatgaaag gaattttgct
      901 aagcggcggt aaacccatca gtcattcctc ttctggcgct caagccatat tttcttcgcc
      961 ataaaaatca ccacggtcag ggcaaacaga ataaacagtt cggtgacaaa ccagaaagta
     1021 ccgcctgtta acatgctgcc tgcccaggaa taaacaatag tcgccggtaa ttgaccaagc
     1081 ccggtggcga taaaaaacga gcgaaaacgt attgaagtca gaccggcagc atagctgatt
     1141 ggatcgaaag ggacaaaagg caataaccga cagaccagga tggtgtgttt gccgtagcga
     1201 gtgaaaaagc cgtccatact gtcaagcacg gttttgccgg ttaatttttc caccacttcg
     1261 cggcccatca ctctggcgat aaaaaagcac agcgccgcgc cggccatcga actggtccac
     1321 gacagcaagc cgccccagaa cgcgccaaac agcgacgcat tggcaaaggt gatcaaaaac
     1381 gcaggtagcg gtgcagcaat ggcctgtaaa atcatcaaca agaacgagac aactgccgcc
     1441 agtgcgccgt aagactgaat aaagcgttct ataccctgtt ggtccacggc ggcaaacgcc
     1501 gcaacgctgc gattgataaa ctcatggacg ccgggaagca gcgcccatgc gagcagcata
     1561 gcgaacaaca ggatgatgag ggttatacgg tagtaccaga ttttacgcga ttgtatcttc
     1621 atcatccttt atcttggctt tattcggtga ttttaaaggt aaccgttgcc agcgtgttat
     1681 ccgccgggag aactgaggca ttgcctttga atttcacttc gccgtgtttt tccaccgcac
     1741 cataagtgta tgttgcattg ctgacgatgc cgaccgggca gctatccaga cacaccaggc
     1801 aacccgtttt cttctcttct gctgccgtta aattaccgcc aaagcgcatg tccagtttct
     1861 tgccattact gtcaacaatc acttcatcga aggaatacgc ttttgccgcc ccttgccagt
     1921 tgaccgaaat atccagtttg ctgcctgtga catgagtcgt ttctttatta tccatcgtca
     1981 tgttttcgcc cggcgtacca cctgcctctt tcagggcttc ataaaatgct ttcgggggcg
     2041 cataagccat aaacagcgat ttatgtccgt tggagccatc tttaaacaca ataccgtgac
     2101 gagtgtcgtc ggtgaggaaa cgaccattaa tttgcaccag catggttacc gtatggttat
     2161 tggcatcgac gcgcagcggt tggctgttcg aaagtccgtc gtattccact tttgccgccg
     2221 cattctcttt ttgatcgcag ccgctaagcc cggcaacagc cagtaagcag agtgcagcca
     2281 gtcctttttt ccatgacact gaataatgtt gcgacatcgt tctatccttc attttttggg
     2341 tgagtaagtg ggctgcggcg cgaagcagac agatccacat gtttgtgtcg ggcgtagagt
     2401 tttgcgagct ggatgagaat aaacagcgcc aggcccgcca gacagagttg taaaataaag
     2461 cgcagcgtaa tgccttcatt ggcgagatcg cttgccatca cggtataaat aacaataccg
     2521 ggcagggtcg tgagtgccga aataagggta taaggccaga aggcgatggt ggtgaatccg
     2581 taagcgtaat tttgaatatt gtaaggaaac aacgggatta agcgggtcag aataagaaaa
     2641 tcaataccgt tacgcgcaat gcctttttca atggcctgga aggtatggct atggcccacg
     2701 tatttcagca gtaaatcacg tcccagccag cgcgccagca ggaatgagca cgaggaggcc
     2761 agcgttgcgg caattaatga gagttgtgtc cctaagagcg ggccaaaaac tattcctcca
     2821 gcgatcacca gtatgcttcc tggtaacaga aagagggggg cattgatgaa taacagaata
     2881 tagaggctat aaccgaacaa tccgctctgg cgaatgagtg tctgtaagtg gggtaaatcg
     2941 gtgagcagat cgaataaacc gaaagcgtgg atagcgtaaa tgaccagcgc aaaaataaga
     3001 caggcaaaca gaaagttacg ctcagcgttc atcgtggagt ttcctatatc aaacttgata
     3061 atagagaagt ggtcagacaa ctaaaaacgc agtgacggta taacaaagga cggcaggcaa
     3121 caaatcatta ctgacagttt gttgattttt ctcaaggtta attctcctga cccagtttga
     3181 gccaggagag ctgctaaatt agcggcggaa ggtcgcccag acgggggcgt gatcggacgg
     3241 tttttccatg ctgcgaattt catagtcgat gccggtttct acgcaacatt ctgccagcgg
     3301 ttggctggcg agcagcaggt cgatgcgcag accacggtta tcgtcaaaac cttttgagcg
     3361 gtaatcaaac catgagaaac gatctgctgt ttgcggatta gcatggcgga aggtatcgac
     3421 caccccccag ctcatcagcc tgtccatcca ttcgcgctct tccggcagga aagagcattt
     3481 accggtacgc agccagcgct tacggttttc ttcgccaatg ccgatatcca gatctgtagg
     3541 gctgatattc atatcgccca taatcagtac cggattatca cgtttgagtt cggtttccag
     3601 gtagttttgc agattctgat aaaactgcgc ttttgccggg aattttatcg gatggtcgcg
     3661 gctttcaccc tgcgggaagt aaccgttgat cacggtgaca ttacccagca gtgaggggat
     3721 ttccgccata ataatccgcc gctgcgcctc ttcgtcgtca ccgggaaagc cgcgacgcac
     3781 ggcaatcggc gtctctttgg tcagcagcgc cacgccataa tggcctttct gcccgtgata
     3841 aaacacgttg tagccgagct tcgccacctc ttcgagcgga aacatatcgt catgaacttt
     3901 tgtctcctgc aggccaatca catccggttg gtgcttttcg acgatggctt caagctggtg
     3961 aggtctggcg cgcaggccgt tgatattaaa agagacaaat ttcatagtcg ctgccattta
     4021 ttcagataaa gagtgcgtgg atggtagcag aatttcgcgt tgcttaccgc ctgttgttaa
     4081 gtgatttgat cgatggttgt taatgatgtc aacgatggcg caaaaaatgc ccgcttttgg
     4141 tgcgcgctgc gtcagaatgg cgcagtaatt tccagtaaat ttcgtcaatg atcacaattc
     4201 tagaattata ttttacttct gcataacatt gcgtttttta ttctttttat tcacccacat
     4261 cgcaaacgta ttcactttat atgcacttta aatgcatatg gtttggttat aactccttga
     4321 tttccagtta gcctccgccg tttatgcact tttatcactg gctggcacga accctgcaat
     4381 ctacatttac agcgcaaaca ttacttattg ttaacataca aataacgaat tatttactgt
     4441 agaggtcgct atgtctcagc caattacgcg tgaaaacttt gatgaatgga tgatacctgt
     4501 ttacgctccg gcacccttta taccggtacg tggcgaaggt tcgcgcttgt gggatcagca
     4561 ggggaaagag tatatcgact tcgcgggtgg cattgcggtg aacgcgctgg gccatgcgca
     4621 tccggaactg cgtgaagcgc tgaacgaaca ggcgagtaag ttctggcata ccggcaacgg
     4681 ttacaccaac gagccggtac tgcgactggc gaaaaaattg atcgacgcca cgtttgccga
     4741 tcgcgtcttc ttttgtaact ccggtgcgga agccaacgaa gcggcgctaa aactggcgcg
     4801 taaattcgct cacgaccgct acggcagcca taagagtggc atcgtggcgt tcaaaaatgc
     4861 gtttcatggt cgcacgctgt ttactgtcag tgcgggtggg cagccagcct attcacagga
     4921 ttttgcgcca ctgccggcgg atattcgtca tgctgcatat aacgatatta actctgccag
     4981 cgcgctgatt gacgacgcca cctgtgcggt gattgtcgag cccatctagg gcgaaggcgg
     5041 tgtggtgcca gccagcaacg cgtttttaca aggtctgcgt gaattgtgtg accgccacaa
     5101 tgccttgctg atttttgatg aggtacaaac cggcgtcggg cgcactggtg agctgtatgc
     5161 ctatatgcac tacggcgtga cgcccgatct gttaaccacc gccaaagcgc tgggcggcgg
     5221 tttcccggtc ggtgcgttgt tggcaaccga agagtgcgcc cgcgtgatga ccgttggcac
     5281 tcatggcacc acctatggcg gtaatccgct ggcctcggcg gtagcaggca aagtgctgga
     5341 gctcatcaac acaccagaga tgcttaatgg cgttaaacag cgtcacgact ggtttgttga
     5401 gcgtcttaat actattaatc accgctatgg tttgttcagt gaagttcgcg gcttaggttt
     5461 gttgattggc tgtgtactga atgccgatta cgccgggcaa gcgaaacaga tctctctgga
     5521 agcggcgaaa gcaggcgtga tggtactgat tgcgggtggc aacgtggtgc gttttgcgcc
     5581 tgcgctcaat gtcagcgaag aagaggtgac gaccggactg gatcgttttg cagctgcttg
     5641 cgaacacttt gttagccgag gttcatcatg atggtcatcc gtcccgttga gcgctcagat
     5701 gtctcggcgc tgatgcagct tgccagcaaa acgggcggcg gcctgacgtc gcttcccgcc
     5761 aatgaagcca cgctttcggc gcgtatcgaa agggcaatca aaacctggca aggtgaactg
     5821 cccaaaagcg agcagggcta tgtgttcgtg ctggaagata gcgagacagg caccgtggcg
     5881 gggatttgcg ctattgaggt ggcggttggg ctgaacgatc cctggtacaa ctatcgcgtc
     5941 ggcacgttgg ttcacgcctc aaaagagctg aatgtctata acgcattgcc gacgctgttt
     6001 cttagtaacg atcacaccgg cagcagcgag ctgtgcacgc tgtttctcga cccggactgg
     6061 cgcaaagagg gcaacggcta tttgctgtcg aaatcgcgct ttatgtttat ggcggctttt
     6121 cgcgacaagt ttaatgacaa agtggttgct gaaatgcgcg gggtgattga cgaacacggc
     6181 tattcaccgt tctggcaaag cctcggtaaa cgcttctttt cgatggattt tagccgcgcc
     6241 gattttctct gcggcaccgg gcaaaaggca tttattgcag aactgatgcc gaaacatccg
     6301 atctataccc actttttatc ccaggaagcc caggacgtca tcggtcaggt acatccgcaa
     6361 accgcgcctg cccgcgcggt gctggagaaa gaaggttttc gttaccgcaa ctatatcgac
     6421 atcttcgacg gcgggccaac gcttgagtgt gacatcgacc gcgtgcgcgc catccgtaaa
     6481 agtcggctgg tggaagtggc agaagggcag cctgcgcagg gcgattttcc ggcctgcctg
     6541 gtcgccaatg aaaattatca ccatttccgc gtggtgctgg cgcgtaccga tccggcaacc
     6601 gagcgtttga ttttaaccgc cgcacaactg gatgccctca aatgccacgc aggggatcgc
     6661 gttcgtctgg tgcgcctgtg cgcagaggag aaaacagcat gactttatgg attaacggtg
     6721 actggataac gggccagggc gcatcgcgtg tgaagcgtaa tccggtatcg ggcgaggtgt
     6781 tatggcaagg caatgatgcc gatgccgctc aggtcgaaca ggcttgtcgg gcagcccgtg
     6841 cggcgtttcc gcgctgggcg cggctctcat tggctgaacg tcaggtcgtt gtcgaacgct
     6901 ttgccggatt gctggaaagc aataaagccg aattaaccgc gattattgcc agagaaacgg
     6961 gtaagccgcg ctgggaagcg gcaaccgaag tgacggcgat gatcaataaa atcgcgatat
     7021 caattaaggc gtatcacgtt cgtaccggcg agcagcgtag tgaaatgccg gacggtgcgg
     7081 cgagcctgcg acatcgcccg cacggcgtgc tggcggtgtt tgggccgtat aatttccctg
     7141 gtcatttgcc gaacggacat atcgttccgg cattgctggc aggtaacacc attatcttta
     7201 aacccagcga actgacaccg tggagtggcg aagcggtaat gcgtttatgg cagcaggctg
     7261 gcttgccgcc gggcgtgctg aacctggtgc agggcgggcg tgaaacgggt caggcgctga
     7321 gtgcgctgga ggatctcgac ggtttgctgt ttaccggtag cgccaatacc ggctaccagc
     7381 tgcatcgcca gctctccggt cagccggaga aaattctcgc ccttgagatg ggcggtaata
     7441 atccgctaat tatcgatgag gtggcggata tcgacgcggc tgtccatctg accattcagt
     7501 cggcgtttgt cacagccggg caacgctgca cctgcgcccg ccgtttattg ctcaaaagcg
     7561 gagcgcaggg cgatgcgttt cttgctagtc tggttgccgt cagccagcga ttaacgccgg
     7621 gcaactggga tgacgaaccg cagccgttta ttggcgggct gatttctgaa caggccgcac
     7681 agcaggtggt tactgcctgg cagcaactgg aagcgatggg cggacgaacc ctgcttgcgc
     7741 cgcgcttatt acaagcaggg acatcgttgc tgacaccagg gatcattgaa atgacgggcg
     7801 ttggcggtgt gccggatgaa gaggtgttcg ggccgttttt gcgcgtctgg cgttatgata
     7861 cttttgatga ggcgattcgg atggcgaaca acactcgctt cggactcttt tgcggtctgg
     7921 tttcccccga gcgggaaaaa ttcgatcaac tgttgctgga ggcgcgggcg gggattgtta
     7981 actggaacaa accgcttact ggtgctgcca gtaccgcccc attcggcggc attggtgctt
     8041 ccggtaacca tcgccccagc gcctggtatg ccgcagatta ctgcgcatgg cctatggcga
     8101 gcctggagtc ggactcgtta acattgcccg ccacgcttaa ccccgggctg gatttttccg
     8161 atgaggtggt gcgatgaacg cctgggaagt taatttcgac gggctggtag ggctgacgca
     8221 tcattacgcg ggcctgtcgt ttggtaatga agcctctacc cgtcaccgtt ttcaggtgtc
     8281 taacccgcga ctggcggcaa aacagggctt actgaaaatg aaagcccttg ccgatgcggg
     8341 attcccccag gccgtgattc cgccgcacga gcgtccgttt attccggtgc tgcgtcagtt
     8401 gggattcagt ggtagcgatg agcaggtact ggaaaaagtt gcacgccagg caccgcactg
     8461 gctttccagc gtcagttccg cttcgccaat gtgggtagcc aatgcggcaa cgatcgcgcc
     8521 atctgccgat acgctggatg gcaaagtgca tctcaccgtt gccaacctga acaataaatt
     8581 tcaccgttcg ctggaagcgc ccgtcactga atcgctgtta aaagcgattt ttaaggacga
     8641 agagaaattt agcgtccatt cggcgttgcc gcaggtagcg ttgc
//
LOCUS       DAJTSI010000159         8665 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000159 DAJTSI010000000
VERSION     DAJTSI010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8665
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.159"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..237)
                     /locus_tag="OO327_003440"
     CDS             complement(<1..237)
                     /locus_tag="OO327_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6019854.1"
                     /translation="MVTFETVMEIKILHKQGMSSRTIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPAT"
     gene            853..990
                     /locus_tag="OO327_003441"
     CDS             853..990
                     /locus_tag="OO327_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019855.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            1590..2369
                     /gene="ipaJ"
                     /locus_tag="OO327_003442"
     CDS             1590..2369
                     /gene="ipaJ"
                     /locus_tag="OO327_003442"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCR6019856.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(3316..4245)
                     /gene="virB"
                     /locus_tag="OO327_003443"
     CDS             complement(3316..4245)
                     /gene="virB"
                     /locus_tag="OO327_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCR6019857.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4462..4698)
                     /locus_tag="OO327_003444"
     CDS             complement(4462..4698)
                     /locus_tag="OO327_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6019858.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESTLEHIITIGDLISVVKNSTKSI"
     gene            complement(4702..6603)
                     /gene="ipaA"
                     /locus_tag="OO327_003445"
     CDS             complement(4702..6603)
                     /gene="ipaA"
                     /locus_tag="OO327_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCR6019859.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKAFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6612..7610)
                     /gene="ipaD"
                     /locus_tag="OO327_003446"
     CDS             complement(6612..7610)
                     /gene="ipaD"
                     /locus_tag="OO327_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCR6019860.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRH
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7661..>8665)
                     /gene="ipaC"
                     /locus_tag="OO327_003447"
     CDS             complement(7661..>8665)
                     /gene="ipaC"
                     /locus_tag="OO327_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCR6019861.1"
                     /translation="SQNYQQIAAHIPLNVGKNPVLTTTLNDDQLLKLSEQVQHDSEII
                     ARLTDKKMKDLSEMSHTLTPENTLDISSLSSNAVSLIISVAVLLSALRTAETKLGSQL
                     SLIAFDATKSAAENIVRQGLAALSSSITGAVTQVGITGIGAKKTHSGISDQKGALRKN
                     LATAQSLEKELAGSKLGLNKQIDTNITSPQTNSSTKFLGKNKLAPDNISLSTEHKTSL
                     SSPDISLQDKIDTQRRTYELNTLSAQQKQNIGRATMETSAVAGNISTSGGRYASALEE
                     EEQLISQASSKQAEEASQVSKEASQATNQLIQKLLNIIDSINQSKNSTASQIAGNIRA
                     "
BASE COUNT         2405 a         1385 c         1824 g         3051 t
ORIGIN      
        1 cgttgccggg attttgtaag gatgagcatc ggcgatgcgt tgacgaatat aatcccggta
       61 ttcatccagg agtgaagcaa cagcaggtcg cggcgtatat tttggcggct cagattttgc
      121 ctgcaaataa cgtttaacgg tattgcggga gagccccagt tctctggcaa tcgtccggct
      181 actcattccc tgcttgtgca ggattttaat ttccataact gtctcaaaag tgaccataag
      241 ctctcctgaa tcaggagagc agattacccc ctggatctga tttcaggcgt tgggtgtgga
      301 tcactattgc accgttcgtg acacttgttg actacctgtg cggcatctgg tatcaaggtg
      361 gcgtgaagcc ggttcaggtt ctcatgcgga acgttggaac ctatatcccc ccgataccaa
      421 aggcgaaatg taagtgacaa aatgcaatga acacgttctc cgttaactga ggagcatggt
      481 gataaataaa ggtaaatgca gaaggtgtag agtctcgcag atttctgaca tcatagagcc
      541 taacattaga tgcataacgc tacagattgt agaatctcta caggtgaaat atacactggt
      601 tggcgtttat gtcgcaaaac atgtcattca ggttcatctc accaataagg atatgagtga
      661 agtggaggat aagtgagtct gctgtcagag ttttctggtg tatgtcagta actgcgaacc
      721 atgtctgctt gatatgggag cttatggagg ctctctgtac aaggttaggg agctaacaaa
      781 gcagggataa aaagtccgcc tgatgcaggc acggttcgtt aaatcttttg ccattggtaa
      841 caaaatggtg cgatgaatgt tcaggctatc tttatcttga tggtggttca gctgtttggt
      901 aaagaaatcg ctgtaacaat agaagaactg caggcagtac tggtcccaca tcgtttgctc
      961 agtcaactgg tgaagttccg tagcgcgtaa attaatgctt tgcatgaaac gttactggag
     1021 tggatgaaag aatacctgca tatatcatta ctgcgttgga ttaccagtac aacagattga
     1081 atgaattgga catgttgata gacggcattg aaaagcagct tataagcata gtgcggcaga
     1141 gttaattgga gagcacttcg ccttctgagt attaagtaag catggtggta cctgcggata
     1201 ctatctattc actgtgctcg gtcggcagcc ttactgacca aggagttggg ggagttgata
     1261 acaaagttga aaaaacagct ctcaaccaag gtggttattg taacaatagc aaataagctg
     1321 gtgtgacctg tatcacaata accgattatg gacgtaaata cgacaaaaat atgtcagtat
     1381 caggcggtat taattgctga tgctgatgga cattaactct taataaaagg gtggatgctg
     1441 aaaggttgtt atggtgacgg acgtgatgac gacggaaaag agtatgaggt tttggattta
     1501 aagttcgttc ggggaataag gacccggtca gctaattctc atagtagttc gcattgctgg
     1561 atgcaatact gtgctgcaat tactcgtcga tgtcggaaca acggaagcct tgcaaacggg
     1621 gatgtattca tacaggtgtt atgttatatg gggttttatt gcagggggct ataccaagag
     1681 aatatatgat atctcatcag actgatgtga gggtaaatga gaatagagta aacgaacaag
     1741 gttgtttttt ggcgaggaag cagatgtatg acaattcttg tggagctgcc agtttactct
     1801 gtgctgcaaa ggaattaggg gttgataaaa taccacaata caaagggtct atgtccgaaa
     1861 tgacaaggaa gagttctctt gatttggata atcgttgtga gcgggattta tacttaataa
     1921 ctagtggtaa ttataatccg agaatacaca aagataatat cgcggatgcc ggttattcta
     1981 tgcctgataa gatagtcatg gctacgaggc ttcttggatt gaatgcttat gtcgttgaag
     2041 aaagtaacat tttttcgcaa gtaattagtt ttatttatcc cgatgcaagg gacttattaa
     2101 ttggcatggg atgtaatata gtacatcaac gagatgtgtt atcttctaac caaagagttt
     2161 tggaagctgt tgctgtatcc tttattggag ttcctgtcgg cctccactgg gtgttgtgta
     2221 gacctgatgg aagctatatg gatccagctg taggagaaaa ttattcttgt ttttcaacta
     2281 tggaattagg tgcaagaaga agtaattcta actttatagg atatactaaa ataggtattt
     2341 ccatagttat aactaatgag gctttgtaaa ttgtcgtttt ttatctgtga aggagcttga
     2401 atattttggg gtggggggca taacaaatga tattactgag tacaaattta gctatattgg
     2461 gcggattagt tgtgtgggaa gacgaaatgt ttctcttgaa ataagtgaac tccctcaagg
     2521 aatgattgtg tgcttaaata tttaagtgct ggtaagtttg agaaggctga catattttgc
     2581 agagcaaaca gtgattgact tggatgatat ttttatttta gtcactcgtt gtaattggag
     2641 tatgctttat tttatttgaa aatagatgta ttttataccc gcaatttaaa ctgtaagccc
     2701 tatgaggggt atggatatta taagttactc tttgaccaag cataactttt ggggttagaa
     2761 aaataaatct cttgttaata attaatatga ttttaaaatc aaacttgttt ataattataa
     2821 aataatgtaa gtcggtcatt ttatgggaag attaggagaa taaaacagta attatatttg
     2881 agaaatctgc taatatattc ttaaagcttt tttgatactg tgaacgaagt ctgttaatgg
     2941 tggatttttt gtttatcacc atgtaaaaaa aattgagtag taaaagcgtt gactgctaat
     3001 cctcgctgtt tttattgaaa cgattaaaaa tacgctgaat attttcggcc agtcactcat
     3061 attcacataa aattttgacc atcatgccgc atcccttagt tgcacaattc attttcaatg
     3121 atatgaatat tactaatttc accagataat tttctcgtgg tcagcagggt atgagttttc
     3181 accgtaattt taaggaaaca cagtgcgtgg ggattgaata acagatagcc gacggaatta
     3241 ccccaccggc tgaaagtcaa gttcaacgaa gatttttatt atctgaattg ggcagtttac
     3301 atcagtgttc gatgtttatg aagacgatag atggcgagaa attatatccc gaatagcttc
     3361 atctatttct ctctgaactg ttttgtttat tttagataat gtaaatatta gagttctgtt
     3421 tgttttattc tcttttcttt ttatgtaagt ccgtttgtct ttagaaataa ataacgagtc
     3481 aacttttagc gaagggtttt gctttctgtt tttgcttttc tttatgatgt ttaaaatttc
     3541 ttttttatat atgtccgggg gcaaatttgt atcaaggtct tttatttctt cctttaatat
     3601 tggtagtgta gaactaagag attcattagc cttttcaagt cctttataat aattgaataa
     3661 tatcttgtag tcattaaagt taagttctga cgcgattgga aatagagata ttatttcctg
     3721 tggaacgctt gctgcctgaa aggcacgagt gactttcgcg cgagacagat tctctttttt
     3781 ggctatgtct ttataggaca tccctgatac tttcagaaaa ttaagaccaa taccaagttc
     3841 tcggatgcta tgctcttttg ctgtttgtat atcgtttgct agttttctgg catcaagagt
     3901 agatatatat tcttttgaat atagaacttc aagatctgct cctgcatata ttgcagatgc
     3961 tcttctacga gtgccatcca gaatttcaat tctaccatca atctcccttc ctattacagg
     4021 gaagaattgt tgtagcttta tagtttttat gatatcggct aatgattctt ctgttaggga
     4081 ttcttgatcg cgtacattcg ttttttggtt gacgaaggtt aaatcttgga tccttttatg
     4141 aggaatcttg gctttgataa aggaaacttt attaccagaa tggagtgtaa actctttctt
     4201 ttggccttcc tttatactta acaagtcgtt gcacaaatcc accatatcac accctgttta
     4261 ttcatattga tttatattga caaaagttaa atgcagtgtg agcaagattg tatgtaactg
     4321 ttgatgatag tcaacggtag tcttaaaaat tactatttta atagctggat tgatgcaatt
     4381 tatctttatt ggataaaaga ataatattct tgtggagctc tgatgtgaga gctccataga
     4441 tgaaaaaatg cttatcaatt actatattga ttttgtacta tttttgacta cagaaataag
     4501 atctccaata gtaataatgt gttctaatgt agattcatct atacgtagat tgaactcaaa
     4561 actaatcata tccagcattt caatcagaga aagagaatcg gcagaaagat cttcaataaa
     4621 tttagtttct tcagatagtt tgctgtacgc tataccatag caaaaggcca ctattgataa
     4681 tattttttct tttatcatat tttaatcctt attgatattc tttaatactt ttgataggga
     4741 agtggttaca tcttttgctg ctttatatat attattgttt tcagctgtaa tatcattgtt
     4801 attttttaaa tcagatatat catctgtaag taattctgca gaggtatcgt caatttttga
     4861 caataccttg ctgagggcgc tagatacttc ttttgccttt tcatagatcg catgattttt
     4921 atcaattgta tctgcgttgg tttctgttac gtagtttctt atatttgcat ctgtaacttt
     4981 taatttgttg tttattctat gttctgttgt ggattttgta atgtcctttg atttttcctg
     5041 taactctcgt tcttgtgttc ctgatttagt tccaatgaga gatataagat tacttaggga
     5101 gtttgttact ttttttgaag cttcaaatat cgtttcccgt gttaaatatg tgtctccatt
     5161 tcttgataaa aaatcagtct gaagtacttt tgaatcagtg tttgagttga atttattgat
     5221 ttgcgaagct actctatgag aaagagaagc gctactttta gttgtatctg atgagttatc
     5281 attactgctt gtcactcctt ccgggtggag agggggggtg gttgaagagt tctgtatgtt
     5341 cagaggcctg tgtccccgag aaagagagtt atttgcagat ctttcttgac tactcgtagt
     5401 taacaggtct gaacctattc catccgaatt atctggagta tggttgaaag ccttttctaa
     5461 agctgaatta tttgcaaatg tctggacagt atctgatata gagtgtggta gaacggaagt
     5521 tactttttga actagtgctc tttgtggatt tagtaatgag tttccgttac gggataatag
     5581 atcgtttgtc tgagaagtag gagactgtgc atcattttct gggttctcgt gatagctatt
     5641 gtcatatact ctgttgtcaa aaactctatt atcgtaagtt ctattatcat tatttatatg
     5701 atagtgaatt acaggttggg atgttttctc attggctccg ggggttggtc cgccatctgg
     5761 catatcttcg ggaggtgtag ggaggccatc ttggatagga tgcgaatctt ttatatcttc
     5821 taaaatatca ttaaggactt ttctcaacag ctcaggaaac acggctactg tatttaagtc
     5881 tcttgacagt ttacctatac caaatccttt actatcaata gtatttttac gttgattgcg
     5941 caaactatca aaggcaagtt ttgcctgtgc gtcaactaaa tcatctagtt tgagagatga
     6001 aacagtttca ttacttaact ttaattcagc aagaatatta ttcttgataa atgatgatac
     6061 ttcagtattg aggagttttt gtcccaatga taaagcttca ccagatgtgt gttgttgtgc
     6121 taatagtgca agtctgtgct caatgttttg tcgtaattga tggagtttct tattctttgc
     6181 cgaccatggt ccaaaggcag aatcttggga taatttatct tttataatta gttccgaata
     6241 ctgtgcgaca attttttgaa gtcccttttc gttttcaaaa ttaagaccat tttgatgtac
     6301 taaggagtac aaaacatttg aaaattcgag aatctcgctt ttggcatacg cattcattga
     6361 gttggaataa atgcttctta acacatcttc tttccctgag gaagaaaata aaaaatcaag
     6421 acatttctga ttaaaagacg ttgcaaagtt ttctttttca gacactgatg ctggtgtttt
     6481 tagagaagtt tgcgaactat ggatatcgga tattttgctt tttaactcgc tgtattctgt
     6541 tgatgatggt gaagttgcct tatataagaa tgttggcgct tgagtattat ttacattatg
     6601 catggcgcac ctcagaaatg gagaaaaagt ttatctgtat ctgtacatga gcttattgta
     6661 ctactcaaaa cctttactaa attatcaaaa atactattgg cattactgta tttttgaact
     6721 aaagtttgaa gattattttt cattgtttca tcttcggcag agaatccggc attccatgcc
     6781 tgatattttg cattatctag cacaacctcg ccatttccac ctagattatc taagcttttt
     6841 aacatattgt ctattggggt catgtttata ctgacaacat atcccccgtt tttttgagat
     6901 accttgccga ttgttccacc taattctgta agccatttat ttgcttgttc ctgactaaca
     6961 gtattatttg ctggatatag cggtttatct ttatattttt ccttgagttc ttccaatgcc
     7021 tttttaagcg agttgacttg taatttcacg gagtttccgt cgttacctcc gggagagatc
     7081 cagccggcaa gactggaaag aacagcgcta aaatcttgat acatttgagt atatgaacta
     7141 acggcatgtt catatacttt cagatactgt tcattaatat cattgatgga gtttgcaatt
     7201 ttagcccaca gttctctatg agatatcatt tgatctccat caagctcggc ttctttcgga
     7261 gctgaatgta ataattctct tgcgtcttta ttaattggat attcgtgcct ggaaagaata
     7321 tctaatagtt gagcggattt atttacatcc atgctaatct gagatgaatg taatgctatt
     7381 tcttctagcg atgttttagt caacgttttt tgtgaaagct ctttctttaa tgcctgattt
     7441 gttgttctga tattatgaag ggtgtcgttg agcatagtaa gggaacttac aggatgagaa
     7501 ctggtcgttg tttttatatc agaattaact gtttcggttg atgaaccgtt ggtattgttt
     7561 ggactgaatg atgaggtgga aatactatta gtcagagttg ttatattcat ggttatttcc
     7621 ttacaggaag agccatatat tattatggct cttggatata ttaagctcga atgttaccag
     7681 caatctgact ggctgtcgaa ttctttgatt ggttgatgct gtcaattata ttcaataatt
     7741 tttgtattaa ttgatttgtc gcttgggatg cttctttaga tacttgggat gcttcctctg
     7801 cttgtttact gctggcctga ctgattagtt gttcttcttc ttcaagagca gatgcataac
     7861 gccctcctga tgtggatata ttaccagcaa cggctgatgt ttccattgtt gcacggccaa
     7921 tgttttgttt ttgctgcgca gaaagggtat tgagctcgta agttcttctc tgggtgtcaa
     7981 ttttatcctg caaagaaata tcgggagaac taagagaagt tttatgttca gttgacaggg
     8041 atatattatc tggcgccagt ttatttttac ctaaaaattt tgtgctagag ttagtttgtg
     8101 gtgaggtgat atttgtatct atttgtttat ttaaccctaa tttagaacct gcaagctctt
     8161 tttcaagaga ttgagcagtg gcaaggttct ttcttaaggc tcctttttgg tcgctaatcc
     8221 ctgaatgcgt ttttttggca ccgatacccg ttatacctac ttgtgtgact gctccagtaa
     8281 tgcttgatga tagggctgcc aggccttgcc gaacaatgtt ctctgcagct gattttgtag
     8341 catcgaacgc aatcaatgac aattgagagc ccaatttagt ttctgcagtg cggagagcag
     8401 aaagtagaac ggctacacta ataattaaag aaacagcatt agaagaaaga ctggaaatat
     8461 ccagagtgtt ctctggagta agggtgtgac tcatctctga aagatctttc atctttttgt
     8521 cagtaaggcg agcaatgatt tctgaatcat gctgaacctg ctctgataac tttaaaagtt
     8581 gatcatcatt taatgtggtt gttaatacgg gatttttacc gacattaagt ggaatatgcg
     8641 ctgcaatctg ctgataattt tgtga
//
LOCUS       DAJTSI010000160         8648 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000160 DAJTSI010000000
VERSION     DAJTSI010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.160"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(770..1966)
                     /gene="hemY"
                     /locus_tag="OO327_003448"
     CDS             complement(770..1966)
                     /gene="hemY"
                     /locus_tag="OO327_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR6019862.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1969..3186)
                     /gene="hemX"
                     /locus_tag="OO327_003449"
     CDS             complement(1969..3186)
                     /gene="hemX"
                     /locus_tag="OO327_003449"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6019863.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PQGDTPAAAPQGE"
     gene            complement(3208..3948)
                     /gene="hemD"
                     /locus_tag="OO327_003450"
     CDS             complement(3208..3948)
                     /gene="hemD"
                     /locus_tag="OO327_003450"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR6019864.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3945..4886)
                     /gene="hemC"
                     /locus_tag="OO327_003451"
     CDS             complement(3945..4886)
                     /gene="hemC"
                     /locus_tag="OO327_003451"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR6019865.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5273..7819
                     /gene="cyaA"
                     /locus_tag="OO327_003452"
     CDS             5273..7819
                     /gene="cyaA"
                     /locus_tag="OO327_003452"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR6019866.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPRMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7859..8179)
                     /gene="cyaY"
                     /locus_tag="OO327_003453"
     CDS             complement(7859..8179)
                     /gene="cyaY"
                     /locus_tag="OO327_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR6019867.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8227..>8648)
                     /locus_tag="OO327_003454"
     CDS             complement(8227..>8648)
                     /locus_tag="OO327_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR6019868.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1968 a         2236 c         2226 g         2218 t
ORIGIN      
        1 cagttgacgg ggtatctgct gcgctggcca tgagaggaag agcggcggcg acagcaacaa
       61 caagacgttt gggtgaaaac gaaaattcca tgcaaaatgc tccggtttca tgtcatcaaa
      121 atgatgacgt aattaagcat tgataattga gatccctctc cctgacagga tgattacata
      181 aataatagtg acaaaaataa attatttatt tatccagaaa atgaattgga aaatcaggag
      241 agcgttttca atcctacctc tggcgcagtt gatatgtaag gcaggtttat tatatcgcgt
      301 tgattattga tgctgttttt agttttaacg gcaattaata tatgtgttat taattgaatg
      361 aattttatca ttcataataa gtatgtgtag gatcaagctc aggttaaata ttcactcagg
      421 aagttattac tcaggaagca aagaggatta cagaattatc tcataacaag tgttaaggga
      481 tgttatttcc cgattctctg tggcataata aacgagtaga tgctcattcc atctcttatg
      541 ttcgccttag tgcctcataa actccggaat gacgcagagc cgtttacggt gcttatcgtc
      601 cactgacaga tgtcgcttat gcctcatcag acaccatgga cacaacgttg agtgaagcac
      661 ccacttgttg tcatacagac ctgttttaac gcctgctccg taataagagc aggcgttttt
      721 ttatgcatca gaaaggtccc ggaggtgctt tcctccgggt gagaaggaac tactgtggcg
      781 ggttattctg caacgttaac atcaaaccgt cgcgacgcat cgctgcagct tcttccggct
      841 tgtgcagtct gtccagcgcg tcggcaagcc atgcgtaatc gtaggcgtcc ggacgttgtt
      901 tcagcgccgc gcggaaggcg agcgatgctt cctgccattc accgtgcttc atcagtgact
      961 gacccaatgt gctccacaac agcgggcgat caccgacgtt tttgatttgc tggcgtagca
     1021 ctttttccag ctgttccggg ttattggttt tcagacgcgg gatcggcagc agtaggcgat
     1081 cgtcatactg gcgtttcagg ccgtcgatga taatttgctg ggcagtatca tgatcgtcac
     1141 attcaataag atgttccgcc attgccacct gcaacgctac ctgatgacgc gttttccggc
     1201 tttggttttt ccaccagtta cgcaaacctt cactaccgtt atcggcacgc gcctgatcca
     1261 tcaggccaat ccatgcctgt tgttccagca ttgcacgatg ttcttcatca ccaacatggg
     1321 ctttcgccat tgatgggatg atatccagca gagaactcca tgcccctgtg cggatatacg
     1381 cctgttccgc cagacgtaat acttcaggat ggcgtggcgt aacttccagc agcttatcca
     1441 cgccgtggcg tgcagcatgg ttttcattac gggccagttg cagacgtaca cgggtgattt
     1501 ctaccggaat ggtgtcattg ccggccagct ccgctgcgcg ttccagatgt tggttggcgc
     1561 gtgcttcatc accacgttgt tgcgcggctt cggcagccag tagatagttc accaccggtt
     1621 gttcagcgtg atcggcattt ttcgccatca gcttttcaac ttgctgataa tcaccttccg
     1681 ccagtttcag cagcgcctgt tcggtctgct tacgcgcacg gcgacgctta cgtccgacaa
     1741 accatccacg ggtgtgcgcg ccagtgcgga agatccgccg cagtagccac tcaatggcaa
     1801 acagcactac catcgccaga atcaatatga tcgccaggcc cgtgacgctg gtttcgatat
     1861 tgtagttgtc ggtctggatc agcacataac cctgatgacc ggcaatcatc gggccaacca
     1921 ctatccccgc aatcagcaaa acaaagagta ataacacttt tagcatggtt attctccttg
     1981 cggcgcggct gccggagtat caccttgcgg tgcaggtgca ggtgcaggtg caggtgcagg
     2041 tgcaggtgca ggtgcaggtt tagcttccgt tgtccccgct gccggttgtg ccagcaggtt
     2101 acgcacgcga gtctgcatca gtttttccag catcgcctgg ctttgcaggg tttccggaag
     2161 atccatcgag atattttgct ggcttaactg gtccacctcg tcgaggaacg ctttggtggt
     2221 ggcatcatca gtatcgtagt aagcacgtac ccaggtggag acgttctcca gcgcctggcg
     2281 ataagtctct tcctgatgac gcggtacagc ttgtgctgcg accagcaggc gagagcgaat
     2341 attttcgcgc agatagatat cctgatttgg cgctaacagc ggtacggcgg tgtcatcacg
     2401 acggcgaatc gtaatgaagt tgtccataaa gttctgccag cttttttgca gattgatacg
     2461 ccattcgctg atggaactgg aaagctcttc accgtctgaa tccatcggcg aaccatcgct
     2521 gtcattatcg gccagacgca ggttatctac ctgatttgaa agctgattaa gcttaaggat
     2581 gatgccgtca taatccacct gcgatactgc agaaaggctg gcgatatcat cggtaattgc
     2641 ccgacgaacg gtaatcagac tcgggtcatt catatccgcc aggctggcgt ctgcactttt
     2701 cagcaacgct gcagaggtcg tgacgtcctg atcgctccac agcttccgtc cggcgagttt
     2761 caccagaaaa tcggcctgag ccagcagcca ggttttagca tcgctgccgg aaatggtggc
     2821 gatcttttgt tggacttcat ccaactgttt tgccagcgtt tcttgctgac gattcgcctg
     2881 cttaagctgc gcagcttgtt gcttaataat gccttccagc tcggcttttt ggctctcctg
     2941 ggctttttgc aatgccgtca gttggttagc cagggcatcg ctggtggcgg tctgattgac
     3001 ggcctgttgt ttaccccagc catacaaacc gacacccgcc gccagagcaa tagcgatagc
     3061 caccgcgctg agaatcaatg cagtattgtt cttactcttt ttttctgttg cgacaggttg
     3121 tgacgtggtg tccacggcct ccctggtctc ttcaaccacg gcggaggttt tttcttgttc
     3181 cgtcattatg gcttcctgtt atgagagtta ttgtaatgcc cgtaaaagcg catcgttgtc
     3241 agcgttatcg gcgaccttaa tgtcttgcca gcccagttcc cgggcgagtt tcgccaaacg
     3301 ctcactgacg accaatagtc gacagtgtag taaccagtgc tcacgatacc attgtgggat
     3361 cagcgaccag agttgctgca acatttcacc gctggtaaca acgaccatcg tcacctcgcg
     3421 ggattgccag cgcatcgctt cttctgcacc atcgtaatgg attgcgcatc gttgataaca
     3481 ttcacaaaaa gtgacctcag caccgcgcac cgtcagggta tccccaatta gctcacgacc
     3541 gccattgcca cgtaatatca gcgcacgttt gcccgcaata ttttgtaatt caggtaattg
     3601 tagcaagact tcgctgattt cccgatcctg tgggtagaga atcttctgtc cgcttacggt
     3661 atgtagcgcc agtgcggtgg tgcgtccaat ggcgaaataa gtaggtagtt ggggccattt
     3721 acgatcttgc tgatgcagct gtgattgggc aaaagcaacc gcgtgttgcg agagggcaaa
     3781 caacagatcg ctctccccca gcgctgccag ttgatcagca agttgcggta attgtcgacc
     3841 cggagaaaac tcaatcagcg gaaaatgcca ggccacctgc cccagtgtgc gcagacggct
     3901 cactaactct tctccagcgg gagacgggcg ggtgaccagg atactcatgc cggggcctct
     3961 ccgttataga cttcagcgag gatctcgcgc gcgccgttat tcagtagctc ttctgccagc
     4021 gaaatcccca tttgttcggc atcttgcggc gcaccgcggc gttcaccgcg aataatctgc
     4081 gaaccgtccg gcgcgccgac cagcgcacat agccagattt cgccatcaat aagctcggcg
     4141 tagctaccaa ttggcacctg acatccgcct tcgagacggg tattcatggc gcgttctgcg
     4201 gtaacgcgca gtgcagtttc gtggtgattc agcgcggcaa gcagctcgcg agtgcgtgta
     4261 tcatcaaggc ggcattcaat accgactgca ccttgtccta ccgccggaag agaaatctcg
     4321 ggtggtaacg cggcgcgaat gcgggactcc agacctaaac gttttagtcc ggctacggca
     4381 agaatgatgg catcgtattc gccgttatct agtttgctca ggcgagtgcc gacgttgccg
     4441 cgcagggagc ggataatcag atccgggcgg cgttcagcca gttggcactg gcgacgtaaa
     4501 ctggacgtcc cgacgatact gcctgctggt aacgcatcca gactgtcata gttattggac
     4561 acaaaggcat cgcgaggatc ttcacgctcg caaatagtga ccagccccag accttgcggg
     4621 aattcaaccg gcacatcttt cattgaatgt acggcgatat cagcgcgatt ctccaggagc
     4681 gcgacttcca gctcttttac aaataagcct tttccgccta ctttcgccag cggcgtatca
     4741 agaatcacat cgccgcgcgt caccatcggt accagttcaa cgaccaggcc cggatggctc
     4801 gccatcaact tgtctttgac atagtgtgcc tgccagagtg caagtgggct ttggcgtgtg
     4861 gcaattctta aaacattgtc taacatgctt gttaccgtca ttatcatccg tggtccatcc
     4921 taacatcctt gccagagtga tgtcagtgtt gtggtgaaac gtagacgcct gcgcaaaccg
     4981 taaaatgagg tctggcagtg gatcctgaca ggcgtttcac gccgtcgtaa taaggaattt
     5041 acagagaata aacggtgcta cacttgtatg tagcgcatct ttctttacgg tcaatcagca
     5101 aggtgttaaa ttgatcacgt tttagaccat tttttcgtcg tgaaactaaa aaaaccaggc
     5161 gcgaaaagtg gtaacggtta cctttgacat acgaaatatc ccgaatgccg cgtgttaccg
     5221 ttgatgttgg cggaatcaca gtcatgacgg gtagcaaatc aggcgatacg tcttgtacct
     5281 ctatattgag actctgaaac agagactgga tgccataaat caattgcgtg tggatcgcgc
     5341 gcttgctgct atggggcctg cattccaaca ggtctacagt ctactgccga cattgttgca
     5401 ctatcaccat ccgcgaatgc cgggttacct tgatggtaac gttcccaaag gcatttgcct
     5461 ttacacgcct gatgaaactc aacgccacta cctgaacgag cttgaactgt atcgtggaat
     5521 gtcagtacag gatccaccga aaggtgagct tccaattact ggtgtataca ccatgggcag
     5581 cacctcgtcc gtagggcaaa gttgttcctc tgacctggat atctgggtct gtcatcaatc
     5641 ctggctcgat agcgaagagc gccagttact acaacgtaaa tgcagcctgc tggaaagctg
     5701 ggccgcctcg ctgggtgtgg aagtcagctt cttcctgatt gatgaaaacc gcttccgtca
     5761 taatgaaagc ggcagcctgg ggggcgaaga ttgtggctcc acccagcata tactgctgct
     5821 tgacgaattt tatcgtactg ccgtgcgtct cgccggtaag cgtattctgt ggaatatggt
     5881 gccgtgcgac gaagaagagc attacgacga ctatgtgatg acgctttacg cgcagggcgt
     5941 gctgacgcca aatgaatggc tggatctcgg tggcttaagc tcgctttctg ctgaagagta
     6001 ctttggtgcc agcctttggc agctctacaa gagtatcgat tccccataca aagcggtgct
     6061 gaaaacactg ctgctggaag cctattcctg ggaatacccg aacccacgtc tgctggcgaa
     6121 agatatcaaa cagcgtttgc acgacggcga gattgtatcg tttggcctcg atccatactg
     6181 catgatgctg gagcgtgtta ctgaatacct gacggcgatt gaagatttca cccgtctgga
     6241 tttagtacgt cgctgcttct atttaaaagt gtgcgaaaaa ctcagccgtg aacgcgcctg
     6301 tgtaggctgg cgtcgcgcag tgttgagcca gttagtgagc gagtggggtt gggacgaagc
     6361 tcgtctggca atgctcgata accgtgctaa ctggaagatt gatcaggtgc gtgaggcgca
     6421 caacgagctg ctcgacgcga tgatgcagag ctaccgtaat ctgatccgct ttgcgcgtcg
     6481 caataacctt agcgtctccg ccagtccgca ggatatcggc gtactgacgc gtaaactgta
     6541 tgccgcgttt gaagcattgc caggtaaagt gacgctggta aacccgcaga tttcacccga
     6601 tctctcggaa ccgaatctga cctttattta tgtgccgccg ggccgggcta accgttcagg
     6661 ttggtatctg tataaccgcg cgccaaatat tgagtcgatc atcagccatc agccgctgga
     6721 atataaccgt tacctgaata aactggtggc gtgggcatgg tttaacggcc tgctgacctc
     6781 gcgcacccgt ttgtatatta aaggtaacgg cattgtcgat ttgcctaagt tgcaggagat
     6841 ggtcgccgac gtgtcgcacc atttcccgct gcgcttacct gcaccgacac cgaaggcgct
     6901 ctacagcccg tgtgagattc gccatctggc gattatcgtt aacctggaat atgacccgac
     6961 agctgcgttc cgcaatcagg tggtgcattt tgatttccgt aagctggatg tcttcagctt
     7021 tggcgagaat caaaattgcc tggtaggtag cgttgacctg ctgtaccgca actcgtggaa
     7081 cgaagtgcgt acgctgcact tcaacggcga gcaatcgatg atcgaagccc tgaaaactat
     7141 tctcggcaaa atgcatcagg acgccgcacc gccagatagc gtggaagtct tctgttatag
     7201 ccagcatctg cgcggcttaa tccgtactcg cgtgcagcaa ctggtttctg agtgtattga
     7261 actgcgcctt tccagcaccc gccaggaaac cgggcgtttc aaggcgctgc gcgtttctgg
     7321 tcaaacctgg ggcttgttct tcgaacgcct gaatgtatcg gtacagaagc tggagaacgc
     7381 catcgagttt tatggcgcta tttcgcataa caaactgcac ggcttgtcag tgcaggttga
     7441 aaccaatcac gtcaaattac cggcggtggt ggacggcttt gccagcgaag ggatcatcca
     7501 gttctttttc gaagaaacgc aagacgagaa tggctttaat atctacattc tcgacgaaag
     7561 caaccgggtt gaggtgtatc accactgcga aggcagcaaa gaggagctgg tacgtgacgt
     7621 cagtcgcttc tactcgtcat cgcacgaccg cttcacctac ggctcaagct tcatcaactt
     7681 caacctgccg cagttctatc agattgtgaa ggttgatggt cgcgaacagg tgattccgtt
     7741 ccgaacaaaa tcgattggca acctgccgcc cgccaatcag gatcacgata cgccgctatt
     7801 acagcagtat ttttcgtgat gagcgtgccg gaaagcgagg cttatccggc atgcaatctt
     7861 agcggaaact gactgtttca cccgcctgtt gcgtcgccgc ctgttccagc aaatcccaga
     7921 aggtttcgcc gctgcgatcg caaatccatt catcgccttt caggtcaaaa tggtagccgc
     7981 cctgtttggt tgccagccac acctggtgca gcggctcctg gcggttaata atgattttgc
     8041 tgccgttttc aaaggtaatg gtcagtacgc cgccgttgat ttcgcagtcg atatcgctgt
     8101 cgccatccct gtcgtccaga cgctcttcaa tggtcagcca cagttgatca gccaggcgat
     8161 gaaattcact gtcgttcatt gttgtatcct gtttttaagt gatggcggca gtatagcggc
     8221 atggggtcag ggcttcaaag tttgcacctc tgctgctgcg ttccggcatg attcatccgt
     8281 caccggaata atgatgtctc tgttttgaac ttcacgtacg ttctcctctc tgacacgagt
     8341 tttcgccggg cagggggagg gcgtaatcct tacctctgta tcggtccatg tataaacatt
     8401 gcgatcgtca taagcgaagc gatcgttttt agttagcgaa ccgttaactg gaataatttg
     8461 caagctcttt gaccgtaatt ggcggataaa gtaagcatgc tgtttgccgc tattaaatga
     8521 gcgatcaatg accaggtctg gcgtatccag cgggattatc tcaagctggt cttcaccaaa
     8581 tgtcgataat tttgttacat acagccgatc tttatattgc gcaatattgc cagtaatcag
     8641 atggaatt
//
LOCUS       DAJTSI010000161         8627 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000161 DAJTSI010000000
VERSION     DAJTSI010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8627
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.161"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..615
                     /gene="mxiE"
                     /locus_tag="OO327_003455"
     CDS             <1..615
                     /gene="mxiE"
                     /locus_tag="OO327_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCR6019869.1"
                     /translation="VRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALVEHSHAE
                     KCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLVLYLLRT
                     IEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVFLHNQTI
                     TSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            602..2302
                     /gene="mxiD"
                     /locus_tag="OO327_003456"
     CDS             602..2302
                     /gene="mxiD"
                     /locus_tag="OO327_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCR6019870.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDIAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIISIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            2321..3388
                     /gene="mxiC"
                     /locus_tag="OO327_003457"
     CDS             2321..3388
                     /gene="mxiC"
                     /locus_tag="OO327_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCR6019871.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            3401..5461
                     /gene="mxiA"
                     /locus_tag="OO327_003458"
                     /gene_synonym="virH"
     CDS             3401..5461
                     /gene="mxiA"
                     /locus_tag="OO327_003458"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCR6019872.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            5474..5875
                     /gene="spa15"
                     /locus_tag="OO327_003459"
                     /gene_synonym="spaK"
     CDS             5474..5875
                     /gene="spa15"
                     /locus_tag="OO327_003459"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCR6019873.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            5879..7171
                     /gene="spaL"
                     /locus_tag="OO327_003460"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             5879..7171
                     /gene="spaL"
                     /locus_tag="OO327_003460"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCR6019874.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            7264..7602
                     /gene="spa13"
                     /locus_tag="OO327_003461"
                     /gene_synonym="spaM"
     CDS             7264..7602
                     /gene="spa13"
                     /locus_tag="OO327_003461"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCR6019875.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            7589..8467
                     /gene="spa32"
                     /locus_tag="OO327_003462"
     CDS             7589..8467
                     /gene="spa32"
                     /locus_tag="OO327_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCR6019876.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
BASE COUNT         2874 a         1209 c         1738 g         2806 t
ORIGIN      
        1 gtccgaaatc aaaatataaa attcagtgat aatgtgaact atcattatcg ctttaacatt
       61 aatagttgtg caaaatttct tgctttttgg gactatttca gtggtgctct agtagagcat
      121 agccatgctg aaaaatgtat tcatttctat catgagaatg atcttcggga tagttgtaat
      181 accgaatcga tgcttgataa gttaatgtta agattcatct tttcatctga ccagaatgta
      241 agtaatgcac tggctatgat acgaatgact gagtcatatc atttggttct ttatcttctg
      301 agaactattg agaaagaaaa ggaggtaaga ataaagagtc tgacagagca ttatggtgtg
      361 tcggaggctt attttagatc tttatgtaga aaagctttag gggcaaaagt caaagaacaa
      421 ctgaacactt ggcggttagt aaatggttta ttagatgtat ttttgcacaa tcaaacaatc
      481 acttcagctg ccatgaataa tgggtatgca tcaacatccc atttttcaaa tgagataaaa
      541 acacgactag gcttttctgc aagagaatta tcgaatatta cttttttagt gaaaaaaata
      601 aatgaaaaaa tttaatatta aatctttgac tctcttgatt gtattgttac cattgatagt
      661 taatgctaat aatattgact ctcatttatt agaacaaaat gacatagcga aatatgttgc
      721 tcaaagtgat acggtaggat ctttttttga acgtttttct gcgttattaa attatcctat
      781 tgttgttagc aaacaagctg caaaaaagag aatatctggg gagtttgatt tatctaatcc
      841 agaagaaatg cttgaaaaac tgacattact tgtgggactc atatggtata aagatggaaa
      901 tgcattatac atatatgact ctggagaatt aataagcaaa gttattttgc tggagaatat
      961 atcacttaat tatcttattc agtatctgaa ggatgctaac ctttatgatc acagatatcc
     1021 tataagagga aacattagtg ataaaacatt ctatatatct ggtcctccgg cattggttga
     1081 actggtagct aatacagcaa ctttgcttga taagcaggtt tcttctattg ggacagataa
     1141 agttaatttt ggggttataa aattaaaaaa tacatttgtt agtgacagaa cttataatat
     1201 gagaggggag gatattgtca tcccaggggt ggctactgtt gtagaacgtt tattaaataa
     1261 tggtaaggct ctgagtaaca gacaagccca aaatgatccg atgccaccct ttaatattac
     1321 acagaaagta tctgaggata gtaatgactt ttcatttagt tcagttacca atagttctat
     1381 attagaggat gtatcactta ttgcatatcc agaaaccaat tctatattag tcaaaggtaa
     1441 tgatcagcaa atacagatta ttcgcgatat tatcactcag ttagatatag ccaaacgaca
     1501 tatagaatta tcattatgga taatcgatat tgataaatct gaattaaata accttggtgt
     1561 taattggcaa ggaacggctt catttggtga ttcgtttggt gcgtcattta atatgagctc
     1621 atcagcatcg ataagcactt tggacggcaa taagtttatt gcttcagtta tggcattaaa
     1681 tcagaaaaag aaagcaaatg tagtttcaag gccggtgatt ttgacacaag agaatattcc
     1741 ggctatattt gataataaca ggacttttta tgtgtcatta gttggtgagc gcaattcttc
     1801 gttggagcat gttacgtatg gaacattgat aaatgttata cctcgctttt catctcgggg
     1861 acaaattgag atgtctttaa cgatagagga tggaacagga aatagtcagt caaattacaa
     1921 ttataataat gaaaatacat ctgtccttcc agaagttgga aggaccaaaa tcagtacaat
     1981 agcgagagtt ccacaaggca aaagtttgtt gataggtggt tatacgcatg aaacgaactc
     2041 taacgaaata atatctattc cttttctctc ctctattcct gtaatcggta atgtgttcaa
     2101 gtataaaaca agtaatatca gtaatattgt aagggttttt ctgattcaac cccgtgagat
     2161 aaaggagtca agttattata atactgcaga atataaaagc ctcatatctg aaagagaaat
     2221 acaaaagact acacaaataa tcccatctga aactactttg ctggaagacg aaaaatcatt
     2281 ggtttcatac ttaaattact aactataaag taggtgatgt atgcttgatg ttaaaaatac
     2341 aggagttttt agctctgcat tcattgataa gttgaatgca atgacaaatt cagatgatgg
     2401 agatgagact gctgatgcag agcttgattc tggcttggct aatagcaagt atattgactc
     2461 atctgatgag atggcttccg ctctttcgtc atttataaac agaagagacc ttgagaaact
     2521 gaaaggaaca aatagtgata gtcaggaacg tattttagat ggggaagaag atgaaattaa
     2581 tcacaagatt tttgatttaa agagaacgtt aaaagataac cttcctctag atcgggattt
     2641 tatagacaga ctaaagagat attttaaaga tccaagtgat caagtcttag cattaaggga
     2701 acttttgaat gaaaaagatc ttactgctga acaagtcgaa ttattaacta aaattattaa
     2761 tgagataata tcaggtagtg aaaaaagtgt taatgctgga ataaattcag ctatacaggc
     2821 taaattattt ggcaacaaaa tgaaacttga accacagctt ttgcgtgcat gttatcgtgg
     2881 ttttatcatg gggaacatat caacaacaga tcagtatata gaatggcttg gtaattttgg
     2941 ttttaatcac agacatacaa ttgtgaattt tgtagagcag tcactgattg tagacatgga
     3001 ttctgagaaa ccgagctgta atgcttatga gtttggtttt gtgttatcta aattaattgc
     3061 aattaagatg attagaactt cagacgtaat ttttatgaag aaactggaat cctcaagctt
     3121 gctaaaagat ggcagtttaa gtgcagagca gctattgcta actttattat atatttttca
     3181 atatccaagt gaaagtgagc aaattcttac ttctgttata gaagtatcac gagccagtca
     3241 tgaggattct gtagtgtatc aaacatatct atcttctgtt aatgaaagtc ctcatgatat
     3301 atttaaaagt gaaagtgaaa gagaaattgc gatcaatatt ctacgagagc ttgtcacaag
     3361 tgcatacaag aaagagcttt ctagataaca ggagataaaa gtgatccagt cttttcttaa
     3421 gcaagtaagt actaagcctg aattaattat attagtgctt atggttatga tcattgctat
     3481 gttgatcata ccattaccta cataccttgt tgatttttta atagggctta atattgtact
     3541 tgctattctc gtttttatgg gatcatttta tattgaaaga atattgagtt tttctacatt
     3601 cccttcggtt ttgttgataa cgacattatt tcgtctcgct ttatcaataa gtactagtcg
     3661 actaatttta gttgatgcgg atgcgggtaa aattattact acatttggcc agtttgtcat
     3721 tggtgatagt ctggctgtag gttttgttat tttttcaata gtaactgttg tgcagttcat
     3781 tgttataaca aaaggatctg aaagggttgc ggaggtagct gctcgctttt ctcttgacgg
     3841 tatgccgggg aaacaaatga gcattgatgc ggatttaaag gctggaatta ttgatgccgc
     3901 aggagctaaa gaaagacgca gtattctgga acgtgagagt caattgtatg gttcatttga
     3961 tggagcaatg aagttcatta aaggtgatgc aattgctggc atcattatta tttttgtcaa
     4021 tttaatagga ggtatttctg ttggtatgag tcaacatgga atgtccctat ctggtgcatt
     4081 atctacttat accatactaa ctattggtga cggattggtc tctcagattc ctgctctgtt
     4141 aatttctata agtgcaggat ttatcgttac aagagttaat ggtgatagcg ataatatggg
     4201 acgtaacata atgtcccaga tatttgggaa tccttttgtt ctcattgtta catcagctct
     4261 tgccttggcg atagggatgt tgccaggttt tccgtttttt gtttttttcc tgatagcagt
     4321 tactttgacg gctttattct attataaaaa ggtcgtagaa aaagaaaaaa gtctgtctga
     4381 gtctgattct agtggctata ctggtacatt tgatattgat aatagccatg actcatcttt
     4441 ggcaatgata gaaaatctgg atgctattag ttcagaaacc gttcctttaa ttttattatt
     4501 tgccgaaaat aagataaatg caaatgatat ggaaggtctt attgaaagga taagaagtca
     4561 gttctttatt gattatggtg tcaggcttcc aactatttta tatagaacaa gcaatgagct
     4621 taaggtcgat gatattgttt tgttaataaa tgaggtgcgt gccgatagtt ttaatatata
     4681 ttttgacaaa gtgtgtatta cagatgaaaa tggagatata gatgctctag gtattcctgt
     4741 tgtttcaact tcatataatg aacgtgttat ttcttgggta gacgtttcat atacagaaaa
     4801 tctaactaat attgatgcta aaattaaaag tgctcaagat gaattttatc accagttgtc
     4861 acaagcttta ttaaacaaca taaatgagat ttttggtata caagaaacaa aaaatatgtt
     4921 agatcagttt gaaaatcggt atcctgatct attaaaggaa gtcttccgac atgtgactat
     4981 acagagaatt tctgaggtat tacaaagatt gcttggagaa aatatttctg ttcgcaattt
     5041 aaaacttatt atggagtctt tggcgctttg ggctcccaga gaaaaagatg tcataacatt
     5101 agttgaacat gtccgtgcat cactttctag gtatatttgt agtaaaatag ctgtttctgg
     5161 tgagattaaa gttgtgatgc tttccggata tattgaggat gcaataagaa aagggataag
     5221 gcaaacctct ggtggctctt tcttgaatat ggatatagag gtttcggatg aggtaatgga
     5281 aactttagca catgctttga gagaattgag aaatgcaaaa aaaaatttcg ttcttttggt
     5341 atcagtagat atacgtaggt ttgttaaaag acttatagat aacagattta agagtatact
     5401 cgttatatcg tatgctgaga ttgatgaagc atataccatt aatgtattaa agactattta
     5461 gtgaggttta aatatgagta acattaattt agttcaatta gttagagata gtcttttcac
     5521 gattggttgt cctccatcaa ttattacaga tttagacagc cattcagcga ttaccatttc
     5581 tttggatagt atgcctgcta ttaatatagc tcttgtcaat gagcaggtta tgttatgggc
     5641 taattttgat gcaccaagtg atgtaaaact tcagtcatct gcttataaca tattaaattt
     5701 aatgttaatg aactttagtt atagcattaa tgagctggta gagcttcatc gttctgatga
     5761 gtacttacag ttaagagttg ttattaaaga tgactacgtt cacgatggga ttgtttttgc
     5821 agagattctg catgagtttt atcagaggat ggaaatctta aatggggtct tataatcaat
     5881 gagctataca aaattgctca ctcaattatc ttttcctaat agaatctcgg ggccaatctt
     5941 ggaaacaagt cttagcgatg tttcgattgg tgagatttgt aacattcagg ctggaattga
     6001 aagtaatgaa attgttgcaa gagctcaggt tgtaggattt catgatgaaa aaacaatatt
     6061 aagcttgatt ggaaattctc gtggactttc acggcaaacg ttgattaagc ccactgccca
     6121 gtttcttcat acgcaagtcg gccgtggatt attgggagca gtagtcaatc ctttagggga
     6181 ggttactgat aagtttgctg ttacagataa cagtgaaatt ctttatcgac ctgtagataa
     6241 tgctcctccg ctatatagtg aaagggctgc aattgagaag ccttttttaa caggtattaa
     6301 ggttattgat tctttactca cgtgtggtga aggacagcga atggggattt ttgcgtcagc
     6361 tggttgtggc aaaacttttc tcatgaatat gctcattgaa catagtggtg ctgatatata
     6421 tgttattggg ttaattggtg agcgaggtcg agaggttact gaaacggttg attatttgaa
     6481 aaactctgag aaaaaaagca ggtgtgtttt agtatatgca acttcggatt actcttcggt
     6541 tgatcgttgt aatgctgcat atatagccac tgctatagcc gaatttttta ggactgaagg
     6601 acataaagta gcgcttttta ttgattcatt aacaaggtat gccagagcat tacgtgatgt
     6661 ggccttagcc gctggagaat cacctgccag aagaggctat ccggtttcgg tttttgatag
     6721 cttacccaga cttcttgaaa ggccaggaaa gttaaaggca ggtggctcta ttactgcatt
     6781 ttacactgtt cttttggagg atgatgattt tgctgatcca ttagctgaag aggtaagatc
     6841 cattttagat ggacatatat atttgagcag aaatctagcc caaaaaggac aatttcctgc
     6901 aattgattcc ttaaaaagta taagcagggt atttacacag gttgttgatg aaaaacatcg
     6961 tattatggcc gctgcatttc gggagctact ttctgaaata gaagagctaa ggacaattat
     7021 tgactttggt gaatacaaac cgggggagaa tgcctctcag gataaaatct acaacaaaat
     7081 atctgttgtt gagagttttc tgaagcaaga ttatcgactc ggttttactt atgagcagac
     7141 aatggagctt attggtgaaa caattagata aggttttaaa aatcaaagat aaatatcaac
     7201 ggtctgttaa attaattgaa gcacacatat taacactaaa aaaaaaactc tttgtatcga
     7261 gatgtggagg cattagataa aaggattata tacttcctgc aactggagaa tgacttagaa
     7321 cctgtaggcg ctcagagtgt tagtcagctt ttcaatacaa gacgtaagat cgctatagta
     7381 aaaaagcata ttatccaata tcagtctgaa cgaattcttc taaagggaag aattgaagaa
     7441 attcaaaaag atattgatga ggcaaatgct tctaaaagaa aactgcttca taaagagagt
     7501 aaaatatgta aacggattgg cttgattaaa agaaataact ttgccaaaca gttgatactt
     7561 gatgagttat cacaagagga tatgaagtat ggcattagat aatataaacc taaattttag
     7621 tagtgacaaa cagatagaaa aatgtgagaa actatcttca atagataata ttgatagtct
     7681 cgttttgaag aagaagagga aggttgaaat tcctgagtac tctttaattg catctaacta
     7741 ttttactatt gataagcact ttgaacataa gcatgataaa ggagaaattt atagtggcat
     7801 taaaaatgcg ttcgaactta gaaacgaacg agcgacatat tctgatattc cagaatcaat
     7861 ggccattaaa gaaaatattt tgataccaga tcaagatatc aaagcaaggg aaaaaataaa
     7921 tatcggcgat atgaggggga tcttttcata taataagagt ggaaatgcag acaagaactt
     7981 cgaaagaagt catacttctt ctgtaaaccc tgataatctg ctagaatctg ataatagaaa
     8041 tggtcaaatt ggtttaaaaa atcatagctt gtctattgat aagaatattg ctgacatcat
     8101 ttctttacta aatggaagtg ttgctaaatc atttgagctg cctgtaatga ataaaaatac
     8161 tgcagacata accccatcca tgtcattgca agaaaaatca atagttgaaa atgataaaaa
     8221 tgtttttcaa aaaaatagtg aaatgactta ccactttaaa cagtgggggg ctggacattc
     8281 tgttagtatt tcagtggagt ctggttcttt tgttctaaaa ccgtcagatc aatttgtagg
     8341 aaataaactt gacttaattt tgaaacaaga tgctgagggt aattacagat ttgatagcag
     8401 tcaacataat aaggggaata aaaataatag tacaggatat aatgaacaga gtgaagaaga
     8461 atgctaagaa ttaaacattt tgacgctaac gaaaaactac agattttata tgcaaagcaa
     8521 ctctgcgagc gtttttcgat tcagacattc aaaaataaat ttacaggcag tgaaagttta
     8581 gtcactctta cttccgtgtg tggggattgg gtaattcgta ttgatac
//
LOCUS       DAJTSI010000162         8456 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000162 DAJTSI010000000
VERSION     DAJTSI010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.162"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..352
                     /locus_tag="OO327_003463"
     CDS             <1..352
                     /locus_tag="OO327_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR6019877.1"
                     /translation="SGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKRL
                     DKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRPV
                     PELPEKYRPKKPRE"
     gene            complement(389..2467)
                     /gene="flhA"
                     /locus_tag="OO327_003464"
     CDS             complement(389..2467)
                     /gene="flhA"
                     /locus_tag="OO327_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCR6019878.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(2541..3551)
                     /gene="hypE"
                     /locus_tag="OO327_003465"
     CDS             complement(2541..3551)
                     /gene="hypE"
                     /locus_tag="OO327_003465"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR6019879.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(3548..4669)
                     /gene="hypD"
                     /locus_tag="OO327_003466"
     CDS             complement(3548..4669)
                     /gene="hypD"
                     /locus_tag="OO327_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR6019880.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(4669..4941)
                     /gene="hypC"
                     /locus_tag="OO327_003467"
     CDS             complement(4669..4941)
                     /gene="hypC"
                     /locus_tag="OO327_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR6019881.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(4932..5804)
                     /gene="hypB"
                     /locus_tag="OO327_003468"
     CDS             complement(4932..5804)
                     /gene="hypB"
                     /locus_tag="OO327_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR6019882.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(5808..6158)
                     /gene="hypA"
                     /locus_tag="OO327_003469"
     CDS             complement(5808..6158)
                     /gene="hypA"
                     /locus_tag="OO327_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6019883.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            6370..6831
                     /gene="hycA"
                     /locus_tag="OO327_003470"
     CDS             6370..6831
                     /gene="hycA"
                     /locus_tag="OO327_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR6019884.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            6956..7567
                     /gene="hycB"
                     /locus_tag="OO327_003471"
     CDS             6956..7567
                     /gene="hycB"
                     /locus_tag="OO327_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR6019885.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            7604..>8456
                     /locus_tag="OO327_003472"
     CDS             7604..>8456
                     /locus_tag="OO327_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005092744.1"
                     /note="catalyzes the oxidation of formate to carbon
                     dioxide and molecular hydrogen; formate hydrogenlyase
                     comprises of a formate dehydrogenase, unidentified
                     electron carriers and a hydrogenase (subunit 3); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR6019886.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALRRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAYH
                     TLENIGII"
BASE COUNT         1888 a         2305 c         2276 g         1987 t
ORIGIN      
        1 gtccggcaag cgtatctctc gtgaaaaact gacgattaaa aaaatgatcg atctttatca
       61 agcgaaatgc ccgcaggcgt cagcggagcc ggagcattac gaggcgttgt ttgtttacgc
      121 gcaaaagcgg ctggataaat gtgtgttcgg cgaagagaaa ccagcctgta agcagtgtcc
      181 ggtgcactgt taccagcctg caaagcgtga agagatgaaa cagattatgc gttgggccgg
      241 gccgagaatg ctgtggcgtc atccgatctt aaccgtgcgt catctcatcg atgacaagcg
      301 tccggtgcca gaactgccgg aaaaatatcg tccgaaaaaa cctcgtgaat agtatgccgg
      361 acaggcagat ctgtccggca atttgcagtt aaatcaatgc cgatttatca attcccagcc
      421 gcttcatccg tgacagcagg gtcgtgcgtt tcagccccag acgttgcgca gcgcctttag
      481 gcccggcaac cacgctgttg gtttctttca gcacgcgcac aatcaactga tattcatctt
      541 cgccctcctg ggcgacaacc gttgcggcag gcggcgtttc aggctccggt aaagcaatat
      601 ctggcaatga cagctgcagc acgttgccgc gtgttagcaa tactgcgcgc tcaatgacgt
      661 tttccagttc gcgtacgtta cccggccact ccatgttgct caaggtgcgc agcgtctcgg
      721 caggaatgct gtcgatattg cgccccagac gacgggcaat tttgaaggta aaggctttcg
      781 ccagcagcgg aatatcttcc ggacgctcgc gtagtggcgg caggtgaatc gggaatacgt
      841 tcaggcggta atagagatcg ctacggaact ctcggtcggc gaccattttt ttcagatcgc
      901 ggttagtcgc ggcaattaaa cgtacgtccg tctgaatgat tttgttgctg ccgagacgtt
      961 caaactcctg ttcctgcaat acacgcagca acttcggctg taactccagt ggcatatcgc
     1021 ccacttcgtc gaggaacaga gagcttttat ccgccagttc aaaacgacca atgcgctggg
     1081 cgttggcacc ggtaaaagcc ccacgctcat gaccaaacag atcgctttcc agcaatccgg
     1141 caggcatcgc cgcgcagttc attttgacca tgcggcgatt attacgccca ctgagattat
     1201 ggatcgcacg ggcaatcagc tctttacccg agccagtttc accgaggatc agcacggtac
     1261 tgtcactttg cgccaccatt tcaacttgtt taagcacgct gtacatggct tcgctgcggc
     1321 caataatctc gccaaattca ctatcaacat tgttgagctg ctcggtcagg gcgaggtttt
     1381 catcaaccag ccgttctttc agacgatgga tttcctgata ggcgagggcg ttatcgacag
     1441 cgattgccac acgttcggca atctggcgca gcaaattcag attggtagtg gtaaacactt
     1501 tctcttcgca ttgcgccagt ttcagcacgc ccagcatggt gtcgccagac atcagcggta
     1561 acaggcacaa ggtttgaatc tggttgcccc aggtgtcgaa caacatgcgt tcataggggg
     1621 ctaaatcgtc ccgctcgtag agattgatca gcagcatctc tttacttttg aacacgcgtt
     1681 cggtgagggt tccggcttca tcgacttcgc tctgttcgtg ggcgggatgt tgtttatcaa
     1741 gatagtgagt ggagtagatg ttgagtttgt ttttacggtg gctgcgtaag acaatgctga
     1801 tatcgtcaat gtcgaaatag taatggattt ctttggcgac ttcgctgacc agttcgtcca
     1861 tatccaggcg ggaaagcacc gcgttggtaa tggcaaccag gatgcggaag ttatcgcgct
     1921 cccggcataa caactcatag tcgacattgt tgacgacgcg gctctggatt tgttcggtga
     1981 cgacagaaac gatctgcgta aatgtttgca gacgattgaa ctctttttcg ctccagggac
     2041 gctcgtcata acgaataaat tcacagccac caaaaatatg ccctcccgcc gccagtggca
     2101 tcaggcaata gtgaccaaat ttggggtata gcccacctgc ggccagctgc ggccaggttt
     2161 cacaaaattc ttcgtaactg caatgcagcg tatcagggcg cgacaaaatg ctgcgtaccg
     2221 gaccgtgtgc cagaacagtt tcgtcttcat atttaatggg ggtgtctttt tcacgcgacg
     2281 cgtaataaga cgcacgttga gtctgcgctt gccacaacac aatagccgcg ttgtcggcga
     2341 gcgcagaacg ctttaccagt tgcgaaagag cctcacacag cgaggccaga tcgggctgct
     2401 gcaatagtgt ccgagtgatg tcgaacaacc cttgctgtcc gagatcactc atcggtgtat
     2461 atgacatttt gttcttccag gaaagcaccg caaagcggtg caaaaggtca ttgactaact
     2521 ataggagatt tagaatttta ttagcatata cgcggaagcg gttcggcgtg tggtaaatcg
     2581 agggttcgtt tcacgccata cagaccggca agacgaacac ctttacgttc caccacttca
     2641 ccaatcagcg ccgcgtcttt ccccagtgga tgggaatgta acgctgccag cacttgctct
     2701 gccgcgttgc gttcaacggc gatcaccagt ttgccttcgt tggcaaagtt cagggcgtcc
     2761 agtcccagca attcgcaaac gccgcgcacg gcaggtttaa ccggcagcgc tgattcagaa
     2821 atttcaatac cacaaccgca ggctgccgcg aactcatgaa ccaccgcgtt tacaccacca
     2881 cgggtggcat cacgcagcgc tttcacgccg ggaatgtcac gcagcgtctg aataagcggc
     2941 gtcagcaccg cgcagtcgct gaccagttcg ccatccagcc ccagctgctc acgcaggtta
     3001 aggatagtcg ccccgtggtc gccgagtgta ccgctaacca gcaatacatc gcctgcggtt
     3061 agcgtctgtg cgccccagtg aatattcgcc ggaattgcgc ccatgccagc ggtgttgata
     3121 aacagtttat ctgccgcgcc gcgctgcacc actttagtat cgccagtaac gatggcaatg
     3181 cctgccgtgc gggcggtttc tgccatgctg gtcactacgg ctttcagtgt ctccatcggc
     3241 aatccttctt cgaggataaa gccacaggag agatagcgcg gaatagcgcc actgcccgca
     3301 acgtcattcg cggtgccgca aatcgccagc ttgccgatat taccgccagg aaagaacagc
     3361 gggtcaatga cgtaactgtc ggtggagaac gccagacggt cgccttccgc taccagctgc
     3421 gccagatcaa gacgtgcctg atcttcctgc tctgccagcc acgggttggc aaaggcttcc
     3481 ataaacaggc tgttgattaa ttgctgcatc gcctggccgc cgctgccgtg ggcgagttgg
     3541 atattattca cgcttcactc tcctgctgac gatactgata ccacgcggcg cacgctcctt
     3601 cggaggagac catcagtgca ccaaatgcgg tttgaggatt acaggtgtta ccaaacagcg
     3661 ggcattgatg cggcttacat ttgcccgtta atacctcacc acaacgcgcg cgcgggtcat
     3721 cgcagacctg ctgcggtgcc gggcggaaat gtgcttcggc atcgaatcgt tgataatccg
     3781 gcgtcaggtg cacgccagaa gattcaatca cgcctaagcc gcgccattcg ctgtcgccgt
     3841 tgacacagaa cacatcggca atcgcctgtt gcgccagtag gttaccggca tccggtacca
     3901 cccgacgata ctgattctct accttgctgt gggccgctat tttctgctcc accagcatga
     3961 ccacgccttg cagtagatca aggggttcga aaccagcgac caccagcgga cgatgaaaat
     4021 cgctggcgat aaaattatag gcatcagtgc cgataaccat actaacgtgg cccggcgcga
     4081 ggaacgcgtc gataccgtta tccggctgtt ccagcaaact gcgcagcgtc gggataagcg
     4141 taatgtgctg gcagaagaag taaaaattct gcacatcacg cgctttcgcc tgttgcagag
     4201 tgatggcggt ggtcggcatg gtggtttcaa aacctaagcc gaagaacacc actttgcggg
     4261 ttggattctc ctgcgccagt ttcaacgcat ccatcggcga gtaaacgatg cgcacatcgg
     4321 caccgcgtgc ttttgcctgc aacagcgatc cctgtttccc tggtacgcgc atggcgtcgc
     4381 caaaggtaca gaagatgact tccggatggc tggcaatctc cacgcaggtg tcgattctac
     4441 ccatcggcag tacgcacacc gggcaacccg gaccgtggat aaactcaacg ttttcaggca
     4501 gtaactggtc gaggccgaat ttaaaaatgg cgtgggtatg accgccacac acttccataa
     4561 tccgcagagg gcgttcggcg gtgtaagaga gatgtgaagc acgttcgcgc agatgctcaa
     4621 ttaactgcat cacctgttcc ggcgcgcgat attcatcaac aaaacgcatt atttttcctc
     4681 gccatacaac agcgcgccga catccggctc aacgtcaaac atgttttgta aggcgtcgag
     4741 agtgtcgcgt gcttcggctt cattaattac gctcatggca aagccaacgt gaaccagtac
     4801 ccactggccc acgcgcggct gaccgttttc atcgcagctg ccgactaacg ttaaatcgac
     4861 atcgcgctga atgccgcaga cgtcgacttt cgcctggttg ccgtcaatgg tgcggatctg
     4921 gccgggaacg cctatgcaca tcgctgtgtc tccagccagt tcagccactg gtccatccct
     4981 tcgccgctgg tggcggaaat aaggatgatt tcaatttctg gattgacttc gcgggcgcag
     5041 gcgatgcact tctcaacgtc aaagttgaga tacggcaaca ggtcaacttt gttgagcagc
     5101 atcagcgagg cggcggcaaa catatgcgga tatttcagcg gtttgtcttc accttcggta
     5161 acggaaagca ccgctacttt gtgtttttca ccgagatcga agctggccgg gcatacgagg
     5221 ttgccaacgt tttcgataaa cagaataccg ttatcgtcca gtggcagacg cggtgcggcg
     5281 tcggcaatca tctgtgcgtc aagatggcag cctttaccgg tgttcacctg aatcgctggt
     5341 gtaccggtag cgcgaatgcg tgcggcatcg ttcacggttt gctggtcgcc ttcaataacc
     5401 gcgcacggaa cgctgtcttt caggcgcatt aaggtttccg tcagcagggt ggttttaccg
     5461 gaaccagggc tggaaaccag gttgagcacc agttgcttgc gggcagcaaa gcgcgcgcgg
     5521 ttgcgttcag ccagacggtt gtttttgtcc agcacgtcaa tttcgacttc cagcatccga
     5581 cgctggctca tgcccggtgt gtgagtgccc gcttcgccat gaccgtaatg caggtcgcct
     5641 tcttcagtct ggctgggggt aaattcaggc gctttaatgc cggtgatttt catctgcggg
     5701 cgtgccgccg gggcaaatgg cgcgctacga aacgcggaat gagggttatg ttcatcaccc
     5761 tcgatataca ggttgccttc accgcaaccg catgttgtac acatcgctca ctcctggtct
     5821 atttctatcc gccgaatctg taaaccgtcg tctgccacaa tctgtagcat gtcaccatga
     5881 cactgtggac agcggcggac gcgctgggtc agtagcgtca catactgttg gcatgtttca
     5941 caccagcatt cggcctcttg ttcttcgagg tgcagtttac aaccttccgc cacgctgccg
     6001 cggcaaacca gatcaaaaca aaaggcaaga gagctggttt cgacacaaga aaatgcgcca
     6061 attttgagcc agaccccagt tacgcgtttt gcgccgtgtt ttgcggcctg ctgttcgatc
     6121 aattccagtg cccgttggca gagggttatt tcgtgcatat cgcctcccat taactattgc
     6181 cagctacaag caataattgc gccagtgttg attatccctg cggtgaataa tgtcgatgat
     6241 gtcgaaatga cacgtcgaca cggcgacgaa attcatcttt agcttaaaaa tctctttaat
     6301 aacaataaat taaaagttgg cacaaaaaat gcttaaagct ggcatctctg ttaaacgagt
     6361 aacctgacaa tgactatttg ggaaataagc gagaaagccg attacatcgc acagcggcat
     6421 cgtcgcctac aggaccagtg gcacatctac tgcaattcgc tggttcaggg gatcacgtta
     6481 tcgaaagcgc gcctgcatca cgccatgagc tgcgcgccgg acaaagaact ctgtttcgtc
     6541 ctttttgaac attttcgtat ttacgtcacc ctggcggatg gctttaacag ccacaccatc
     6601 gagtattacg tcgaaacaaa agatggcgaa gacaaacagc ggattgcgca ggcgcaactg
     6661 agcattgacg gcatgattga tggcaaggtc aacatccgcg atcgcgaaca ggttctggaa
     6721 cactatctcg aaaaaatcgc tggcgtttac gacagcttat acaccgccat tgaaaacaat
     6781 gtgccggtga atttaagcca actggtaaag ggacaaagcc cggcagcatg agctgaggct
     6841 ttgcccgttt tgcaggcgtt acgcctgttt ggggatgggc gtgtcgatga gtgtcgaaaa
     6901 tgacatttca tcggcatgtt ttcgtcaaaa atgacaatca cctgaggaat gcctggtgaa
     6961 tcgttttgta attgccgact ccacgctctg tatcggctgc cacacctgtg aggccgcctg
     7021 ttcagagacg catcgccagc acggcctgca atcaatgccg cgcctgagag tgatgctcaa
     7081 tgaaaaagaa tctgcgccgc agctctgtca ccactgtgaa gatgcaccct gcgcggtggt
     7141 ctgcccggtt aacgccatca cccgcgttga tggggccgtg cagttgaatg aaagtctgtg
     7201 cgtaagctgc aaactgtgcg gcatcgcctg cccgtttggc gcaattgaat tttccggcag
     7261 ccgtccgctg gatattccgg caaacgccaa taccccgaaa gcgccaccgg caccgcctgc
     7321 tccggcgcgt gtcagcacat tgcttgactg ggtgccaggt attcgcgcga tcgccgtcaa
     7381 atgtgacctt tgtagctttg atgaacaagg tccggcctgt gtgcggatgt gcccgactaa
     7441 agccctgcat ctggtggata acaccgatat cgcccgcgtc agcaaacgta agcgtgagct
     7501 gacctttaac acggactttg gcgatctcac cttgtttcag caggctcaaa gtggagaggc
     7561 taaatgagcg caatttccct gatcaatagc ggcgtggcat ggtttgtcgc cgccgctgtt
     7621 ctggcatttc tcttttcaaa aagcgctaag tggctggata gccggaattg gcggcgcggt
     7681 tggtagtctg tatacggcag ccgcgggctt cactgtactg actggcgcgg ttggcgtgag
     7741 cggtgcgctg tcgctggtaa gctacgatgt gcaaatctcc ccgcttaacg cgatttggct
     7801 gattaccctc ggcctttgcg gtctgtttgt cagcctctat aacattgact ggcatcgcca
     7861 cgcgcaggtg aagtgcaacg gcttgcagat caatatgttg atggctgccg ccgtctgcgc
     7921 cgtcatcgcc agcaacctcg gcatgttcgt ggtaatggcc gaagtcatgg ccttgtgtgc
     7981 ggtgttcctc accagcaaca gcaaagaggg caaactgtgg tttgcgctga ggcgtcttgg
     8041 cactctgctg ctggtgattg cttgctggct gctgtggcag cgttatggca cgctggatct
     8101 gcgcctgctg gatatgcgta tgcaacagct gccgctcggt tccgatatct ggctgctcgg
     8161 tgtgattggc tttggcctgc tggctgggat tattccgctg cacggctggg tgccgcaggc
     8221 acatgctaac gcctctgcgc cagctgccgc gttgttttct acggtagtca tgaaaattgg
     8281 cctgctgggc atgttaaccc tgtcactgct gggcggtaat gcaccgctgt ggtgggggat
     8341 cgcgctgctg gtgctcggca tgatcaccgc gttcgtcggt ggtctgtatg cgttgatgga
     8401 acacaacatc cagcgcctgc tggcttacca caccctggaa aatatcggca ttatcc
//
LOCUS       DAJTSI010000163         8441 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000163 DAJTSI010000000
VERSION     DAJTSI010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.163"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..188
                     /gene="csgC"
                     /locus_tag="OO327_003473"
     CDS             <1..188
                     /gene="csgC"
                     /locus_tag="OO327_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR6019887.1"
                     /translation="SSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSD
                     GQALHLSQQWTPSSEKS"
     gene            309..620
                     /locus_tag="OO327_003474"
     CDS             309..620
                     /locus_tag="OO327_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6019888.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            715..1248
                     /gene="ymdB"
                     /locus_tag="OO327_003475"
     CDS             715..1248
                     /gene="ymdB"
                     /locus_tag="OO327_003475"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR6019889.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            1190..2671
                     /gene="clsC"
                     /locus_tag="OO327_003476"
     CDS             1190..2671
                     /gene="clsC"
                     /locus_tag="OO327_003476"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCR6019890.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2679..3836)
                     /gene="mdoC"
                     /locus_tag="OO327_003477"
     CDS             complement(2679..3836)
                     /gene="mdoC"
                     /locus_tag="OO327_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR6019891.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            4230..5765
                     /gene="mdoG"
                     /locus_tag="OO327_003478"
     CDS             4230..5765
                     /gene="mdoG"
                     /locus_tag="OO327_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR6019892.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5758..8301
                     /gene="mdoH"
                     /locus_tag="OO327_003479"
     CDS             5758..8301
                     /gene="mdoH"
                     /locus_tag="OO327_003479"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR6019893.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         2135 a         2001 c         2201 g         2104 t
ORIGIN      
        1 gcagttcagg gcaaagtcag acgaagcaag aaaagaccct ctcattgcct gctaatcaac
       61 ccattgcttt gacgaagttg agtttaaata tttccccgga agatcgggtg aaaatagttg
      121 ttactgtttc tgatggacag gcacttcatt tatcacaaca atggacgccc tcttcagaaa
      181 agtcttaatt tgttgaaata tcgagcataa gatgaatctg gagagaatgg tctgctgcga
      241 atcagccaac ctgaaagtat ggataacaca accttcaagg atgactaatc attgaggaaa
      301 tagaataaat gttcagacct tttttagact ctcttatgct cggcagtttg ttttttcctt
      361 ttattgccat tgcaggaagc accgcgcaag ggggcgtgat ccatttttat ggccaaattg
      421 tggaaccggc atgtgacgtc agcacccagt catcacccgt agaaatgaac tgcccacaga
      481 atggttctat tccgggcaaa acctactcca gcaaagcgtt aatgagcggc aatgtcaaaa
      541 acgcgcaaat agcctcagta aaagtgcagt atcttgataa acagaaaaag ctggctgtaa
      601 tgaacatcga atataactaa gtttctgaac aactcacgtt gctgagcaga aaaatgcgat
      661 ttaaccaaaa agcctgctgt acacttaaga aacaagaagg tgtaaggagg cgttatgaaa
      721 acgcgtattc atgttgtgca gggtgatatt accaaactgg ccgttgatgt gattgtgaat
      781 gcggctaatc cgtcattaat gggaggcggc ggcgtcgatg gggccattca tcgcgcagcg
      841 ggtccggccc tgctggatgc ctgtttaaaa gtcaggcaac agcagggcga ttgccctacg
      901 gggcatgccg ttattacgct tgcaggcgat cttcccgcta aagccgtagt acacaccgtc
      961 gggccagtct ggcgtggtgg ggaacaaaac gaagaccagc ttttgcagga tgcctatctg
     1021 aatagcctac gactggtggc ggcaaacagc tatacgtcag tggcttttcc tgcaatcagt
     1081 actggggttt atagttaccc tcgtgcggca gcggctgaaa tcgcagttaa aaccgtttca
     1141 gaatttatta cccgtcacgc tttacccgaa caggtatact ttgtctgtta tgatgaagaa
     1201 aacgcccacc tctacgaaag actccttacc caacaaggag atgaatgatt tgccccggct
     1261 ggcgagcgcg gtgctgccac tgtgttcgca acatcccggt cagtgtggtc ttttccctct
     1321 ggagaaaagt ctggatgcgt ttgccgcccg gtatcgtctg gccgaaatgg cagagcatac
     1381 gctggatgtt cagtattaca tctggcagga cgatatgtcg gggcggttac tgttttccgc
     1441 cctgttagcc gcagcaaagc gtggcgttcg cgtccgtttg ttgctggacg acaacaatac
     1501 gcccggactt gacgacattt tgcgcttgct tgacagtcat ccacgcattg aagtccggct
     1561 ttttaatcct ttctcgtttc gcttgctacg tccgcttgga tatatcaccg acttttcccg
     1621 tctcaatcgc cggatgcaca ataaaagttt cactgtcgat ggcgtggtta ccctagtggg
     1681 aggacgaaat attggtgatg cctattttgg tgcaggggaa gagccacttt tttcggattt
     1741 agatgtcatg gcaataggcc ccgtggtaga ggatgttgcc gatgatttcg cccgctactg
     1801 gtattgcaaa tcggtttcac ccttacagca ggtgctggat gtcccggaag gtgaaatggc
     1861 ggatcgcatc gagttacccg cctcctggca taacgatgcc atgacgcatc gttatttacg
     1921 caaaatggaa tccagtccat ttataaatca tctggttgat ggaacgttgc cgcttatctg
     1981 ggcgaagaca cgtttattaa gtgatgatcc ggcgaaaggg gagggcaagg caaaacggca
     2041 ttcactgtta ccgcagcgcc tgttcgatat catgggctca cccagtgaac gcatcgatat
     2101 tatctcttcc tattttgtac cgacacgcgc aggtgtggcg caactcttac ggatggtgag
     2161 aaaaggggta aagattgcga tcctaaccaa ttctcttgcc gctaacgatg ttgctgtcgt
     2221 ccatgccgga tacgcgcgct ggcgcaaaaa attgctccgc tatggcgtgg aattatatga
     2281 actcaagccg acgcgtgaac aaagcagtac gttacacgat cgcggcataa ccggtaattc
     2341 cggtgccagc ctgcatgcta aaacttttag catcgatggt aaaacggtgt ttatcggctc
     2401 tttcaatttc gatccgcgtt caacattgct caatactgaa atgggcttcg tgatagagag
     2461 cgaaacgctg gcacagttaa ttgataaacg ctttattcag agccagtacg atgcggcctg
     2521 gcagctccgt ctggacaggt ggggacggat caactgggtt gatcgtcatg caaagaaaga
     2581 ggttattctc aaaaaagaac ccgccaccag tttctggaag cgggttatgg tcagactggc
     2641 gtcgatattg cccgtggaat ggttattgta aatgtggctt aacgggctgg tgctttatcg
     2701 ttctcacgct taacaaccgg tttaccagag aacaaaaact tcagtaacgg gatgcgtaaa
     2761 tgaatttcat acagaattat cgcaatccct actacgaata tcaggccaca gagaaaacca
     2821 agccagttgg aggtgatgtg cggtgtaatg tatgcgccga aaaacagcgt taacgggtgg
     2881 tgaaccagat agataaacag cgatgcgtta acaaaataag tcacccgcgc tgactggaag
     2941 ttaagcaaac ggtggccaaa ggagaagacc acattcacca tccacaggcc gaggaccatg
     3001 gtgatcaccg actcggtttc gtacatccag gcatcgccac tgccatagcg ctggttgagt
     3061 aaataagcga caaacgccaa tgctgcagcg agggtacagc cacgagacgg cgtggtaaac
     3121 aaggctttaa gatgagggaa aatgaaagcc agtgcgccga ggataaagaa cggtaaataa
     3181 aatagcgttt gcatgacaat aaaattgaac atgccattac tcagaatggg tggatacaca
     3241 ataaaaatcg ttcttcttat taccgcatag ccgatgccga ggcataaaaa aatcaccgat
     3301 agttttacca tcgagaattt tttattcgtt ttatcagaat tttctaaatt atttctgatg
     3361 cgcttaaata tccatacaca cagcgtcgtc atgactacta acaccagtaa aaaccacagg
     3421 tgtgatatta attcccaggc caacgtatta tatttgtcat acaatgatag cccaggccaa
     3481 ctttccgctt tccctttgac atattgcagc ataataaatt gcggtaatgt cagtaggggg
     3541 atggctgtta acatcgggat acctacacgt tcgacacgta ctttccacca ttttttcaag
     3601 ggatagcgta aaaaaagcat gtaggaaaag tatccggata taacgaaaaa tacctgcatg
     3661 cggaacgagt ggatgaagtc attaaaaagg gttagccaca atgacggttc ggcgctattc
     3721 acatgccatg tatggctcga atagattaaa gaaatatgaa aagggatccc taacaacatc
     3781 agccaggcgc ggatggagtc gaggaaatat tcacgttgcg cgggtactgg attcatatat
     3841 ggttaactaa tctcggattt ttcgtcttat ccctgtcggg ttatgccttt aggcttgttg
     3901 ccatagcgac accgacctga ccgcgccagg cgcaggcttc aaggttttta tgcatagcat
     3961 catcgctacc actaaccaga atggaagcgt ctgtaagacg gttgataaat aaatttgctg
     4021 gcaaacccta cacgaagtcg atgcttctgt ctttaggaga agcacggaaa gtgaaaacgg
     4081 ttgcaatcag gtgcttaatc catgagccag cgtgctgaac gataccggga ttcagttgtc
     4141 ggaatggctg gttatccatt aaaatagatc ggatcgatat aagcacacaa agggggaagt
     4201 gcttactaat tatgaaacat aaactacaaa tgatgaaaat gcgttggttg agtgctgcag
     4261 taatgttaac cctgtataca tcttcaagct gggctttcag tattgatgat gtcgcaaagc
     4321 aagctcaatc cttagccggg aaaggctatg aggcgcctaa aagcaacttg ccctccgttt
     4381 tccgcgacat gaaatacgcg gactatcagc agatccagtt taatcatgac aaagcgtact
     4441 ggaacaatct gaagacccca ttcaaactcg agttctacca tcagggtatg tacttcgata
     4501 ccccggtcaa aataaatgaa gtgactgcca ccgcagtcaa acgaatcaaa tacagcccgg
     4561 attatttcac tttcggcgat gttcagcatg acaaagacac ggtaaaagac cttggttttg
     4621 ccggttttaa agtgctttac ccgatcaaca gcaaagataa aaacgatgaa atcgtcagca
     4681 tgctcggggc cagctatttc cgcgtgattg gtgcaggtca ggtttatggc ctttctgccc
     4741 gcggcctagc aattgatacc gctttgccat cgggtgaaga atttccacgc ttcaaagagt
     4801 tctggatcga gcgtccaaaa ccgactgata aacgtttaac catttatgca ttgcttgact
     4861 cgccgcgtgc gacaggtgct tacaaattcg tggttatgcc agggcgtgac acggttgtgg
     4921 atgtgcagtc gaaaatctat ctgcgcgata aagtcggcaa actgggggtt gcaccgttaa
     4981 ccagtatgtt cctgtttggg ccgaaccaac cgtcgcctgc aaataactat cgtccggagt
     5041 tgcacgactc taacggtctg tctatccatg ctggtaatgg cgaatggatc tggcgtccgt
     5101 tgaataaccc gaaacattta gcggtcagca gcttctccat ggaaaacccg cgaggctttg
     5161 gtctgttgca acgaggtcgt gatttctccc gctttgaaga tctcgatgat cgttacgatc
     5221 tccgtccaag cgcatgggtg actccgaaag gggagtgggg caaaggcagc gttgagctgg
     5281 tggaaattcc aaccaacgat gaaaccaacg ataacatcgt cgcttactgg acgccggatc
     5341 agctgccgga gccgggtaaa gagatgaact ttaaatacac catcaccttc agccgtgatg
     5401 aagacaaact gcatgcgcca gataacgcat gggtgcaaca aacgcgtcgt tcaacggggg
     5461 atgtgaagca gtcgaacctg attcgccagc ctgacggtac tatcgccttt gtggtcgatt
     5521 ttaccggcgc agagatgaaa aaactgccag aggatacccc ggtcacagcg caaaccagca
     5581 ttggtgataa tggtgagata gttgaaagca cggtgcgcta taacccggtt accaaaggct
     5641 ggcgtctggt gatgcgtgtg aaagtgaaag atgccaagaa aatcactgaa atgcgtgctg
     5701 cgctggtgaa tgccgatcag acgttgagtg aaacctggag ctaccagtta cctgccaatg
     5761 aataagacaa ctgagtacat tgacgcaatg cccatcgccg caagcgagaa agcagcattg
     5821 ccgaagactg atatccgcgc cgttcatcag gcgctggatg ccgaacaccg cacctgggcg
     5881 cgggaggatg attccccgca aggctcggta aaggcgcgtc tggaacaagc ctggccagat
     5941 tcacttgctg atggacagtt aattaaagac gacgaagggc gcgatcagct taaggcgatg
     6001 ccagaagcaa aacgctcctc gatgtttccc gacccgtggc gtaccaaccc ggtaggccgt
     6061 ttctgggatc gcctgcgtgg acgcgatgtc acgccgcgct atctggctcg tttgaccaaa
     6121 gaagagcagg agagcgagca aaagtggcgt accgttggta ccatccgccg ttacattctg
     6181 ttgatcctga cgctcgcgca aactgttgtc gcgacctggt atatgaagac cattcttcct
     6241 tatcagggtt gggcgctgat taatcctatg gatatggttg gtcaggattt gtgggtttcc
     6301 tttatgcagc ttctgcctta tatgctgcaa accggtatcc tgatcctttt tgcggtactg
     6361 ttctgttggg tgtccgccgg attctggacg gcgttgatgg gcttcctgca actgcttatt
     6421 ggtcgcgata aatacagtat atctgcgtca acagttggcg atgaaccatt aaacccggag
     6481 catcgcacgg cgttgatcat gcctatctgt aacgaagacg tgaaccgtgt ttttgctggc
     6541 ttgcgtgcaa cgtgggaatc agtaaaagcc accgggaatg ccaaacactt tgatgtctac
     6601 attctgagtg acagttataa cccggatatc tgcgtcgcag agcaaaaagc ctggatggag
     6661 cttatcgctg aagtcggtgg cgaaggtcag attttctatc gccgccgccg tcgccgcgtg
     6721 aagcgtaaaa gcggtaatat cgatgacttc tgccgtcgct ggggcagcca gtacagctac
     6781 atggtggtgc tggatgctga ctcggtaatg accggtgatt gtttatgcgg cctggtgcgc
     6841 ttgatggaag ccaacccgaa cgccgggatc attcagtcgt cgccgaaagc gtccggtatg
     6901 gatacgctgt atgcgcgctg tcagcagttc gcgacccgcg tgtatgggcc actgtttaca
     6961 gccggtttgc acttctggca acttggcgag tcgcactact ggggacataa cgcgattatc
     7021 cgcgtgaaac catttatcga gcactgcgca ctggctccgc tgccgggcga aggttccttt
     7081 gccggttcaa tcctgtcaca tgacttcgta gaagcggcgt tgatgcgccg tgcaggttgg
     7141 ggggtctgga ttgcttacga tctcccgggt tcttatgaag aattgccgcc taacttgctt
     7201 gatgagctaa aacgtgaccg ccgctggtgc cacggtaacc tgatgaactt ccgtctgttc
     7261 ctggttaagg gtatgcaccc ggttcaccgt gcggtgttcc tgacgggcgt gatgtcttat
     7321 ctctccgctc cgctgtggtt tatgttcctc gcgctctcta ctgcattgca ggtagtgcat
     7381 gcgttgaccg aaccgcaata cttcctgcaa ccacggcagt tgttcccggt gtggccgcag
     7441 tggcgtcctg agctggcgat tgcacttttt gcttcgacca tggtgctgtt gttcctgccg
     7501 aagttattga gcattttgct tatctggtgc aaaggaacga aagaatacgg cggcttctgg
     7561 cgcgttacat tatcgttgct gctggaagtg ctgttttccg tgctgctggc tccggtacgc
     7621 atgctgttcc atacggtctt cgttgtcagc gcgttccttg gctgggaagt ggtgtggaat
     7681 tcaccgcagc gtgatgatga ctccacttca tggggcgaag cgttcaaacg ccacggctca
     7741 cagctgctgt tggggttagt gtgggctgtt gggatggcgt ggctggatct gcgtttcctg
     7801 ttctggctgg caccgattgt cttctcgttg atcctgtcac cgtttgtttc ggtgatttcc
     7861 agccgtgcca ccgttggtct gcgcaccaaa cgctggaaac tgttcctgat cccggaagag
     7921 tattcaccgc cgcaggtgct ggttgatacc gatcggttcc ttgagatgaa tcgccaacgc
     7981 tcccttgacg atggctttat gcacgcagtg tttaacccgt catttaacgc tctggcaacc
     8041 gcaatggcga cagcgcgtca ccgcgccagt aaggtgctgg aaatcgcccg tgaccgccac
     8101 gttgaacagg cgcttaacga gacgccagag aagctgaatc gcgatcgtcg cctggtgctg
     8161 ctaagcgatc cggtgacgat ggcccgtctg catttccgcg tctggaattc ccctgaaaga
     8221 tattcttcat gggtgagtta ttacgaaggg ataaagctca atccactggc attgcgtaaa
     8281 ccggatgcgg cttcgcaata aaaacgtagt tgcctgatgc gctacgctta tcaggcctac
     8341 atcgttcctg caatttgttg attttgcaag attttgtagg tcggataagg cgttcacgcc
     8401 acatccggca aaaacaacga gccaataaaa ataccggcgt t
//
LOCUS       DAJTSI010000164         8435 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000164 DAJTSI010000000
VERSION     DAJTSI010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.164"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..305
                     /locus_tag="OO327_003480"
     CDS             <1..305
                     /locus_tag="OO327_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCR6019894.1"
                     /translation="LIARWELTAEQAVAQQLKNQVSKGNLIDTGFCIFALSKLAMALS
                     STLDSIPLSMQRQFPDLTPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
     gene            277..2187
                     /locus_tag="OO327_003481"
     CDS             277..2187
                     /locus_tag="OO327_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR6019895.1"
                     /translation="MNISEQQLSNMMMAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLQFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTCDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TRVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGIFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVQLHPARMQLHNR
                     PLKNWQRSCPEE"
     gene            2188..2391
                     /locus_tag="OO327_003482"
     CDS             2188..2391
                     /locus_tag="OO327_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6019896.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2388..3981
                     /locus_tag="OO327_003483"
                     /pseudo
     CDS             2388..3981
                     /locus_tag="OO327_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3971..5476
                     /locus_tag="OO327_003484"
     CDS             3971..5476
                     /locus_tag="OO327_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR6019897.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5513..5860
                     /locus_tag="OO327_003485"
     CDS             5513..5860
                     /locus_tag="OO327_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR6019898.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGCSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5918..6946
                     /locus_tag="OO327_003486"
     CDS             5918..6946
                     /locus_tag="OO327_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR6019899.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            6998..7384
                     /locus_tag="OO327_003487"
     CDS             6998..7384
                     /locus_tag="OO327_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR6019900.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7396..7773
                     /locus_tag="OO327_003488"
     CDS             7396..7773
                     /locus_tag="OO327_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6019901.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7760..8344
                     /locus_tag="OO327_003489"
     CDS             7760..8344
                     /locus_tag="OO327_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6019902.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPMSGPL
                     TQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            8341..>8435
                     /locus_tag="OO327_003490"
     CDS             8341..>8435
                     /locus_tag="OO327_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6019903.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPA"
BASE COUNT         1962 a         1988 c         2597 g         1888 t
ORIGIN      
        1 tactcattgc cagatgggaa ctgaccgcag aacaggctgt cgcacagcag ttaaaaaatc
       61 aggtgtcaaa aggaaatctt atcgataccg gattttgtat ttttgccctt agtaagctgg
      121 cgatggcttt atccagtacg cttgactcca ttcctttgtc catgcaacga cagttcccgg
      181 atttaacgcc gcgtcatatt gatcatctga aaatccttat tgctaaggga gcgaatcagt
      241 gcgcgcgtgc gggggataaa ttaccggatt tactcgatga atatatcaga acaacaactg
      301 agtaatatga tgatggcagt cactatcgca ctgcagccac tggtcagggt attgccggtg
      361 acggctgtag aatgggccga tcaaaattat tacctgccta aagaatcgtc atacggtgag
      421 ggggaatgga aaacgctgca gtttcaggtc gccatcatga actgcatggg caatgacgag
      481 attcgtacag ttaacctgat taaatctgcc cgtgttggtt atacaaaaat ggtgcttggt
      541 gtgatcgggt attttattga gcacaaatcc cgcaacactc ttctttttca gccgacggac
      601 tcagctgctg aagattttat gaagtctcac gtggaagcaa caattcgtga tgttccttgc
      661 ctgaaaaaac tttctccctg gctgggccgg aaacaccggg ataatacgct cacactgaag
      721 cgtttttcct ctggtgtggg gttctggtgt ttgggtggtg ctgctgccaa aaactaccgt
      781 gaaaaatctg tcgatgtggt ctgctatgac gaactctcct cgtttgaacc ggatgtggaa
      841 aaagaaggtt cgccaacact gcttggcgat aaacgtattg aaggttcggt atggccaaaa
      901 tccatacgcg gttcaacgcc aaaaattaaa ggctcctgtc agattgaaaa agctgcgaat
      961 gaatctgcgc atttcatgcg gttttatgtc ccttgccctc attgcgggga ggagcagtat
     1021 ctgaagtttg gcgatgatgc gacgtcgttt ggcctgaaat gggagaaggg taaaccggaa
     1081 acggtgtatt acctgtgtga acataatggc tgcgtgatcc gtcagtctga acttgatcag
     1141 accggggggc gctggatttg tgacaatacc gggatgtgga cgtgtgacgg tctgacgttt
     1201 ttcagtgctt cgggtaatga aattccgccg ccgcgctcca tcactttcca tatatggacg
     1261 gcgtacagtc cgttcaccac cagggtacag atagtctgtg actggctgga tgcactgaaa
     1321 gatcccaacg gcgtgaaaac ttttgtgaac accacgctgg gcgagacctg ggaagaggcc
     1381 gtgggcgaaa aactcgatca ccaggtactg atggataagg ttgtgcctta cacggcaacg
     1441 gtgcctgtcc gggtggttta tctgacggcg ggcattgact cgcagcgaaa ccgttttgag
     1501 atgtatgtct gggggtgggc tccgggagag gaagcctttc tggtggataa aatcatcatc
     1561 atgggacgtc ctgatgagga agagacgctg ttacgtgtgg atgcggcgat caataaaaaa
     1621 taccgccatg cagacggtac cgaaatgacc atttcccgtg tctgctggga tatcgggggg
     1681 atcgatggcg aaatcgtgta tcagaggtca aaaaaacacg gtattttccg ggtactgccg
     1741 gtaaaaggtg cctctgtcta tggcaagccg gtgatcacca tgccgaaaac ccgcaatcag
     1801 cggggtgtgt atctgtgtga ggtggggacg gataccgcaa aagaaattct ctatgcccga
     1861 atgaaggccg atcccacccc tgtgaatgag gccacatcgt atgccatccg ctttcctgat
     1921 aatccggaga ttttttcgca gacagaggca caacaactgg tggcggagga actggtggaa
     1981 aagtgggaaa aaggaaagat gcgtctgttg tgggataaca aaaagcggcg taacgaagcg
     2041 ctggactgcc tggtgtatgc ctatgcggca ttacgtgtgt ccgtgcaacg ctggcaactt
     2101 gatctggctg tgcagttgca tcccgcaagg atgcaactgc acaaccgacc cttgaagaac
     2161 tggcagcgaa gctgtccgga ggagtgaatg gttacagtcg ctgaactgca ggcgctgcgt
     2221 caggcgcgcc ttgatttatt aaccggtaaa cgggttgtgt cggtacagaa agacggtcgc
     2281 aggatcgaat atacggcagc gtccctgaat gagcttaacc gtgcgatcaa cgatgcggag
     2341 tcggttctgg gtacgacccg acgccgccga ccgctgggag tgaggttatg aaacgaacgc
     2401 ctgtcctgat tgatgtgaac ggcgttccgc ttcgtgagag tctcagctac aacggaggcg
     2461 gcgcaggatt tggcgggcaa atggcagagt ggttaccacc ggcgcagagt gccgatgcgg
     2521 ccctgttgcc tgcattgcgt ctggggaatg cccgggcaga tgatctggtg cgcaataacg
     2581 gtatcgcggc taatgcggtg gccctgcata aggatcatat cgtcgggcac atgtttctta
     2641 tcagctaccg tctgaactgg cgttggctgg ggatgcggga gaccacggca aaaagttttg
     2701 tcggggaggt ggaagcagcc tggtcggaat acgcagaagg aatgtttggc gagatcgacg
     2761 tggaagggaa acgcacgttt acggaattta tccgtgaagg ggtgggcgtg catgccttta
     2821 atggcgaaat ctttgtgcag ccggtctggg atacggaaac cacgcagtta ttccgtacgc
     2881 gttttaaagc cgtgagtccg aaacgggtgg acacgccagg acacggtatg gggaaccact
     2941 ttctgcgggc cggggttgag gtcgatcggt atgggcgggc gattgcgtac catatctgtg
     3001 aggatgattt tccgttctct ggtgccggac gctgggagcg gatcccgcgt gagcttccca
     3061 ccgggcgtcc ggccatgctg catatttttg agccggtgga ggacgggcag acccgtgggg
     3121 ctaatcagtt ttacagcgta atggagcgga tgaagatgct ggactccctg caggccacgc
     3181 agcttcagtc ggccattgtg aaggcgatgt atgcagccac aatcgaaagt gaacttgata
     3241 ccgaaaaggc atttgaatat atcgcggggg caccgcaagg tcagcaggat aatccgctta
     3301 ttaatattct ggagaagttc acccgctggt atgacacgaa taacgtgacg ctgggcggtg
     3361 ttaaaattcc gcaccttttt tcctggcgat gcgctgaacc tgcagacagc gcaggattca
     3421 gacaatggat tttcggcgct tgaacaggcg ctgctgcggt atattgcagc cggtcttggc
     3481 gtttcctacg aacagttgtc ccgtgattac tcgaaggtca gttactcaag tgcccgcgcc
     3541 tccgccaatg agtcgtggcg ctattttatg gggcggcgaa aatttattgc ggcccggctg
     3601 gccacgcaga tgttttcctg ctggctggaa gaggcgcttc ttcgggggat tattcgtccg
     3661 ccacgggcgc gttttgattt ttaccaggcg cgttcagcct ggtcacgggc agagtggatt
     3721 agctccggaa gaatggccat tgacgggctc aaggaggtcc aggaatcggt gatgcgcatt
     3781 gaggccggac tgagcacgta tgagaaagaa ctggcactga tgggcgaaga ttatcaggac
     3841 attttccgcc agcaggtcag ggaatccgca gagcgcgaaa aagccggact ctcacgtccg
     3901 gtgtggatag cccaggcgta tcagcagcag atagcggaga gtcgcaggcc ggaagaggag
     3961 acaacatcac gtgagacgta atctttcaca tattatcgct gcggcattca atgagccgct
     4021 gcttctggag cccgcctatg cgcgggtttt cttttgcgcg ctcgggcgtg agatgggcgc
     4081 agcaagcctt tcggtaccgc aacagcaggt acagcttgac gctcccggga tgctggctga
     4141 aacggacgag tacatggccg gaggcaaacg acctgcccgt gtttaccggg tggtgaacgg
     4201 tattgctgta ctgccggtga ccggcacgct ggtacaccgg ctgggtggca tgcggccatt
     4261 ttccggaatg actggctatg acggcattgt cgcctgtctt cagcaggcaa tggcggacac
     4321 tcaggtacgg ggcgtactgc tggacattga cagttctggc gggcaggcca ccggcgcgtt
     4381 tgactgcgct gacatgattt accgtctccg gcagctgaag cctgtctggg cactgtgcaa
     4441 tgacacggcc tgttcagccg ccatgttact ggcatcggcc tgctcccgac ggctggttac
     4501 ccagacatcc cgtatcggct ccattggggt gatgatgggc catgtcagct atgccggtca
     4561 tctggcacag gccggtgtgg atatcacgct gatttacgcc ggatcgcaca aggtggatgg
     4621 caatcagttt gaatccctgc cagcaaaggt tcgccaggac atgcagcagc gcattgatgc
     4681 ggcgcgccgg atgtttgctg aaaaagtggc gatgtatacc ggtttatctg tggatgcggt
     4741 tatgggaaca gaggccgccg tttttgaagg tcagtccggt attgaggccg aactggcgga
     4801 tgaattaatc aatgcgtcgg acgccatcag cgtgatggct gcgacgctga acacacacga
     4861 tacaggaggc actatgccgc aattaactgc aacggaggct gccgcgcagg agaaccagcg
     4921 agtgatgggg atcctgacgt gtcaggaagc gaaaggacgt gaacaactgg ccacgatgct
     4981 ggcagggcaa cagggtatga gcgttgagca ggcccgggcg attctggccg tggcggcacc
     5041 gcaacaatct ggtgtatctg cgcagagtga agccgatcgc attatggcat gtgaagaggc
     5101 gaaaggacgt gaacaactgg cggcaacgct ggcggcgatg ccggagatga cggtggaaaa
     5161 agcccgtccg attctggctg cttcaccgca ggcgaatgcc ggaccctcac ttcgtgatca
     5221 gattatggcc ctggatgagg caaaaggggc agaggcgcag gctgaaaaac tggcggcctg
     5281 tccggggatg accgtggaga atgcccgggc tgtactggcg gcagcctcag gcagggcaga
     5341 accggtttct gcatccacaa ccgccatgtt tgaacatttc atggcgaacc attcaccggc
     5401 agcggtacag ggtggcgtgc cacagacgtc agcagacggt gatgcggacg tgaaaatgct
     5461 catggccatg ccatgaagtc agtgctgacc atcaatatga ggtttttaca atatggtaac
     5521 gaaaaccatc actgaacagc gtgcggaagt acgtattttt gccggtaatg atccggccca
     5581 taccgccaca ggctgcagcg ggatttcctc ggcaacaccg gctctgacgc ccctgatgct
     5641 ggatgaggat accgggaaac tggtggtctg ggacggacag aaggcaggcc atgtggttgg
     5701 catactggta ctgccgcttg aaggtacaga gacggtgcta acgtattaca agtcggggac
     5761 ctttgcgacg gaggccatcc actggcctga gagagtggat acacacaaaa aggcgaatgc
     5821 ctttgccggc agtgccctga gtcacgcagc gctgccgtaa cattttatca ggtcactgcg
     5881 gtagcctgac tgatttctga atgaaaggaa ctgatttatg ggattgttta cgacccgcca
     5941 gttactcggt tataccgaac aaaaagttaa atttcgtgcg ctgtttctgg agctgttttt
     6001 ccgccgtacg gtgaatttcc ataccgaaga ggtaatgctg gacaaaatta ccggaaaaac
     6061 gccggtggcg gcctatgtct ccccggttgt tgaaggaaag gtgttgcgtc accgtggcgg
     6121 tgaaactcgt gtactgcgcc cgggctacgt taagccgaaa cacgaattta attaccagca
     6181 ggcggtggag cgtcttcccg gtgaagatcc ggcccagctg aacggcccgg cttaccgtcg
     6241 cctgcgtatc atcaccgata acctcaaaca ggaagagcac gccattgtcc aggtggaaga
     6301 gatgcaggcg gtgaatgccg tgctgtatgg caaatacacc atggaaggag agcagtttga
     6361 aactgttgag gtcgattttg gccggtcggt gggaaataac attacgcagg caggagggac
     6421 ggagtggagc gctcaggacc gcgatacctt tgatccgacg catgatctgg atgcgtactg
     6481 tgattttgct tccggcacca tcaatattgc cattatggat ggtaaggtct ggcgtctgct
     6541 gaatggcttt aagctgttcc gcgaaaaact ggatacccgt cgtggctcaa attcacagct
     6601 ggaaacggca gtgaaagacc tgggggcggt ggtgtccttc aaagggtatt atggtgatct
     6661 ggcaatcgtg gtggcaaaaa cgtcctatgt ggcagaggac ggtaccgaaa aacgttatct
     6721 gccggacggc acactggtac tgggaaatac ggccgcagag ggtattcgtt gctacggtgc
     6781 cattcaggat gcgcaggcat tgtctgaagg cgtggtggcg tcttcccgtt atccgaaaca
     6841 ctggctgacc gtgggtgatc cggcgcgtga attcaccatg acgcagtccg cgccgctgat
     6901 ggtgctgccg gatccggatg agtttgtggt ggtgcaggtg aaataatccg tgagcgggag
     6961 ctaaatgctc ccgtttttca ttcaatcagg agcgggtatg gctacgaaag aagaaaacct
     7021 gaaacgtctt tgtgagctgg ctgagaggct ggggcgtgaa ccggatgtct ccggtagtgc
     7081 ggcagatatt gcgcagcgag tggcggaact ggaggaagag cttggagatg cgggggaggt
     7141 cgcagagccg gatacgtact caccatcgaa agatgatccg accgcgcata aaggcgagtc
     7201 agttcctgaa cagccaggca ttgttaccgg agacgaaacg gggcaggtta cggtggtggc
     7261 actggcgaca ctgcatacaa aatgtaatgg ttcagtgata tttgtttcac cgggaacgtc
     7321 gtttcgtgtt tctgctgagg tggctgccag catggcggca ggtggtctgg caaaaaggca
     7381 gtaacaggag atgtcgtgga cggtttcgat aatctgtttg atgctgcgct tgccggtgtc
     7441 aatgaagtca ttctccgtga tatggggatc agcgccgtca ttacgtccgg agaacttgag
     7501 gggacacacc tgacaggggt ttttgatgat cccgaaagta tttcttttgt tgccgggggg
     7561 atccggcttg aggattcctc accgtgcctg tttgtgaaaa cggcagatat atcgcagtta
     7621 cgccgtcagg atacgttaac tatcggggat gattctttct ttgtggatcg tattactccg
     7681 gatgacgggg gatgctgtta tatccggtta aggcggggca gtcctgcccc acagaataat
     7741 gccagaatga ggcgatatga tgaagggact tgagaatgcc atccgcaatc tgaacagcct
     7801 cgatacccgc atggtgccac agaccagcgc atgggcggta aaccgtgtgg cggcaaagat
     7861 tgtttctgtc gccacacggc aggttgcgca gaataccgtt gccggggata accaggtgaa
     7921 aggtattccc ctgaaaacgg tcagggagcg tgtcaggctg cttaaagcca gcccctcagg
     7981 aaaaatgtat gcccgcatgc gtgttaaccg gggtaacctg cccgccatta agctgggtac
     8041 cgcacaggtc agactggccc gctcccggca tggcagcaac tcacgtcatc gtggcagcgt
     8101 actgaaagtg gggaaatacc ttttccggga tgcgtttatt cagcaactgg cgaatggtcg
     8161 ctggcatgtg atgcggcgta ttgacggcaa aaatcgttac cccattgatg tggtgaaaat
     8221 cccgatgtcc ggaccgctga cacaggcatt tgaagatgcc cgcgaccgca tcattgctgc
     8281 ggaaatgccg aaacagctgg ggtatgccct gaaacaacaa ctgaggttac atctgagtaa
     8341 atgaaccgac acacacaaat ccgtcagagc gtgctggcac gccttcggga acagtgtgga
     8401 gacagcgcca cgttttttga cgggcttccg gcatt
//
LOCUS       DAJTSI010000165         8377 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000165 DAJTSI010000000
VERSION     DAJTSI010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8377
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.165"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..956
                     /gene="espL1"
                     /locus_tag="OO327_003491"
                     /pseudo
     CDS             1..956
                     /gene="espL1"
                     /locus_tag="OO327_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1128..1235
                     /locus_tag="OO327_003492"
     CDS             1128..1235
                     /locus_tag="OO327_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019904.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1288..2046)
                     /locus_tag="OO327_003493"
     CDS             complement(1288..2046)
                     /locus_tag="OO327_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR6019905.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2333..3262
                     /gene="pfkB"
                     /locus_tag="OO327_003494"
     CDS             2333..3262
                     /gene="pfkB"
                     /locus_tag="OO327_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR6019906.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3363..3653
                     /gene="ghoS"
                     /locus_tag="OO327_003495"
     CDS             3363..3653
                     /gene="ghoS"
                     /locus_tag="OO327_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR6019907.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3759..4619
                     /locus_tag="OO327_003496"
     CDS             3759..4619
                     /locus_tag="OO327_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR6019908.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4660..5196)
                     /locus_tag="OO327_003497"
     CDS             complement(4660..5196)
                     /locus_tag="OO327_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR6019909.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5343..6011
                     /gene="hxpB"
                     /locus_tag="OO327_003498"
     CDS             5343..6011
                     /gene="hxpB"
                     /locus_tag="OO327_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCR6019910.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6174..6764
                     /locus_tag="OO327_003499"
     CDS             6174..6764
                     /locus_tag="OO327_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6019911.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6897..8287
                     /gene="tcyP"
                     /locus_tag="OO327_003500"
                     /pseudo
     CDS             6897..8287
                     /gene="tcyP"
                     /locus_tag="OO327_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
BASE COUNT         2172 a         2004 c         2008 g         2193 t
ORIGIN      
        1 tgctcagacc tgggctgata ggtatgttcg caactgtgca attgctattc tgcccctatt
       61 acctgtggga gttctagggg ctattttcgc gggtattgca tcacgaatgc catttgcccc
      121 tatacatcca tcggcaatgt tgttaataat ggctacaggc cagactcaac agcttattac
      181 attattcaaa cagttgccca tactccctga aaaagaaatc attgaaataa taactgcgca
      241 gaatagcgtt ggtacacctg ctttattttt ggctatgatg aacggacata ctgacaacgt
      301 gaaaatattt atgcaagaaa ttcagtcact ggtagataat cacatcattc atgaagataa
      361 tctggttaaa ttactgcaaa ctaaaagtgc taacgaaaca cctggacttt atatctccat
      421 gttgtatgga ttcgatgaaa taatcgatat ctttctgaat acattagcca ctccaatagc
      481 actaagagct tttaaacaaa aaactggtga tgagtatttt agccatgaaa atacatgatg
      541 gtgagccagg attatacgcc gcaatggaaa ataatcaccc tttgtgtgtc acacggttcc
      601 tctctaaaat taatggcatc gcctttaaat acaagttgag caaagctaac atcatggatt
      661 tattaaaagg agctacagca cagggaaccc ctgcattata catcgccatg agcaagggta
      721 atgaagacgt cgtgttatct tatatatcga cgctgggtgc ttttgcaaaa aaacattctt
      781 ttagtcaaca tcagttattt acactattgg ccgctaaaaa tcatgacaac atgtcagctg
      841 ttcatatagc cattcatcat aagcattata aaactgtaga aacatattat gctgctataa
      901 atgtcatcag ccaaagcctg agttttagtg ctgatgaaat aaagacgtat ttataaccag
      961 ctatttacga tatatatcga tacataattt tatttttttc ataaaacgca gaaacagtga
     1021 ccatgtgcac aacgccaccc gacggctgta ataacaaaaa atcccttgat gcctgtccct
     1081 tttgttacac tccgttatca cgcacaagag atatgcagga cactggtatg ccgactaaac
     1141 gctttgataa aaaacactgg aagatggtgg tggtgctact ggcaatctgt ggcgctatgt
     1201 tgttgctacg ttgggcagca atgatttggg gctgagcagt gacaaacgaa gacagccaga
     1261 ccgcataaca cggtccggca gataatttta catgctgtag cccagcgata aggttgtccg
     1321 acggtcggta tgttccggtg cagactctgg aggttcagag ttccatgtga cgttgtaagc
     1381 caccttcagt ccaaaatgtt cattaatagc aacatttaat gcgctttcgg agttcagcgt
     1441 tgtgtcttcc gcgccaaaga ccgaaacacc ctgcgtaaat ttagcgttgt cagtcaactg
     1501 ccatgcataa gccccggagg catagcccag cggctgggtt tcactggcat tgtcggtata
     1561 tttgtcgtaa cgcacacctg gaccgaattc aaagcggaaa ctgtgtaccg ggccattcaa
     1621 aaactgacga ccataacccg cggtcaacac atcgcgctct cgatagccgt tataacggtc
     1681 tgtcagccag cttgcctgac caaataaata gtcataatca gttaaattaa agcggctacg
     1741 cccgcccgcc gcatattttt ctgaagaacg ctcatcatta gaagaagtat tactggcgtt
     1801 cccccacagc gaccaggcag tggtttgtcc ataccaggtc atggtggtgt cagccgtaag
     1861 tgaggagctt ttcgtattgc ctgattgtgc aagatatcct gcgttcagat taccttcgaa
     1921 aggtttttta gcgctggcag gatcgtccat gacagtaaaa acggaatcat cggcagctgc
     1981 attcagtgac gcaaacatgc cccccgccaa cataacgatg gcaggaactg tcttcaaaag
     2041 cttcatttat caagagtccg tacaacaaaa aaagagacca tcgcggtccc ggaaactttc
     2101 ttaaggatca aagattagcg tccctggaaa ggtaacgaat tataaaaagg cgcgaataac
     2161 ttagcaatgt attcttattt cattttttga ataagcatgt ggcgaaaaca gatttttatt
     2221 tatatatatt tatctgcaaa attttaaata aagctctaat aaatcatatt gttaatttct
     2281 tcactttccg ctgattcggt gccagactga aatcagccta taggaggaaa tgatggtacg
     2341 tatctatacg ttgacacttg cgccctctct cgatagcgca acaattaccc cgcaaattta
     2401 tcccgaagga aaactgcgct gtaccgcacc ggtgttcgaa cccgggggcg gcggcatcaa
     2461 cgtcgcccgc gccattgccc atcttggagg cagtgccaca gcgatcttcc cggcgggtgg
     2521 cgcgaccggc gaacacctgg tttcactgtt ggcggatgaa aatgtccccg tcgctactgt
     2581 agaagccaaa gactggaccc ggcagaattt acacgtacat gtggaagcaa gcggtgaaca
     2641 gtatcgtttt gttatgccag gcgcggcatt aaatgaagat gagtttcgcc agcttgaaga
     2701 gcaagttctg gaaattgaat ccggggccat cctggtcata agcggaagcc tgccgccagg
     2761 tgtgaagctg gaaaaattaa cccaactgat ttccgctgcg caaaaccaag ggatccgctg
     2821 catcatcgac agttctggcg aagcgttaag tgcagcactg gcgattggta acatcgagtt
     2881 ggttaagcct aaccaaaaag aactcagtgc gctggtgaat cgcgaactca cccagccaga
     2941 cgatgtccgc aaagccgcgc aggaaatcgt taatagcggc aaggccaaac gggttgtcgt
     3001 ttcccttggt ccacaaggag cgctgggtgt cgatagtgaa aactgcattc aggtggtgcc
     3061 accaccggtg aaaagccaga gtaccgttgg cgctggtgac agcatggtcg gcgcgatgac
     3121 actgaaactg gcagaaaatg cctctcttga agagatggtc cgttttggcg tagctgcggg
     3181 gagtgcagcc acactcaatc agggaacacg tctgtgctcc catgacgata cgcaaaaaat
     3241 ttacgcttac ctttcccgct aacaaaaaca ttcccccagc attgggggaa tcaccaccaa
     3301 cctgtcggca acgcgtttct ccgactatgc tcaaaagtca tgtgataaca aaggggtgaa
     3361 ctatggccag tggcgatctt gtccattatg tcataaccgt catgttgcat gaggatacat
     3421 tgactgaaat taacgagttg aataattacc tgactcgcga cggttttttg ctcaccatga
     3481 cggatgatga gggaaatatc catgagctgg ggactaacac ttttggactt atcagtaccc
     3541 aaagtgaaga agaaattaga gaactggttt cggggcttac ccaaagtgca accggcaaag
     3601 atcctgaaat caccatcacg acctgggagg aatggaatag caacagaaaa taaatggttt
     3661 ttgggcaata atcagtctgt ggtgtgcgtt agctcgtgtt tttacaccgc attcttgcgc
     3721 taaccttatg atctggcaga caacatggga gagacatcat gtggcaggca atcagtcgtc
     3781 ttttgagcga gcagttaggt gaaggcgaaa tcgaactgcg taatgaactg cctggcggag
     3841 aagtccatgc cgcatggcat ttgcgctatg caggacatga ctttttcgtc aaatgtgatg
     3901 aaagggaact gcttcccggt tttaccgccg aagccgacca actggagtta ctgtcgcgta
     3961 gtaaaaccgt caccgtgcct aaggtttggg cagtaggcgc tgaccgtgac tacagttttc
     4021 tggtgatgga ttatctccca cctcgtccgc tggatgcgca tagcgcattt attcttggtc
     4081 agcaaattgc gcgtttacat caatggagtg accaaccaca atttggcctc gattttgata
     4141 actcgctctc cacaactcca cagcccaaca cctggcaacg tcgctggtca acgttttttg
     4201 ctgaacaacg gattggctgg cagttggaac tggcagcaga gaaagggatc gctttcggca
     4261 atatcgacgc catcgtcgag catattcagc agcgtctggc ctcgcatcaa ccgcagcctt
     4321 ctctgttgca cggcgattta tggtccggca actgtgcgct gggtccggat ggcccgtaca
     4381 ttttcgaccc tgcctgcgac tggggtgacc gagagtgcga cctagcgatg ttaccgctgc
     4441 atactgaaca gccgccacaa atctatgacg gctatcagtc agtatccccg ctacctgccg
     4501 atttccttga acgtcaaccg gtttaccaac tctacacgct gttaaatcgt gcaagattat
     4561 ttggcggtca gcatttggtt attgctcagc agtcattgga tagattatta gcagcatgat
     4621 atgggttgag gataatggcc gctccgtgcg gccttttgat taaataaacc caagcagaga
     4681 aaagaacaca taacctgcag caatacaaat aaccggcagg atatatagtg agaaaaactg
     4741 caggaaaata gtatggtgcg gaacaacaat acgggactca atttgttcac gcgttagccc
     4801 ctcttcccct ttggcttttt ccagaatgag ttgatcttca acaccttccc gcaagaagcg
     4861 cgcctggcga ctcatccggg caccggaatc ctgcaacgcc agtccgacaa aaatcaggat
     4921 gaaaatcacc cagaacataa cgttcacacc accattaaaa ttaggggtcg gggagttata
     4981 ccagaacaga ttcaaaaaag gcgtattcgc ctgcatcata tcgatcatga catgagcaaa
     5041 gtcgagcatg accgcattaa tgccttcctg gttttcctgc cgggtattca taaatttcag
     5101 caaagaaatc aatgtagaga tcaatgcagg tataaaaatc acccaaccca aaatcctttt
     5161 caaaacagca atgcgtccag cttgttgata cgtcatgagt tctccttgat taagacgcgt
     5221 cgtttcactt agtttacctg tagatatctg ttttcgccca ttctttaaag gcgatatgat
     5281 aggcgcttaa tcataagcac ggcttaatac cttacacata atgctctaaa ggagaggttg
     5341 taatgtcaac cccgcgtcag attcttgctg caatttttga tatggatgga ttacttatcg
     5401 actcagaacc tttatgggat cgagccgaac tggatgtgat ggcaagcctg ggggtagata
     5461 tctcccgtcg taacgagctg ccggacacct taggtttacg catcgatatg gtggtcgatc
     5521 tttggtacgc ccggcaaccg tggaatgggc caagccgtca ggaagtagta gaacgggtta
     5581 ttgcccgtgc catttcactg gttgaagaga cacgtccatt attaccaggc gtgcgcgaag
     5641 ctgttgcgtt atgcaaagaa caaggtttat tggtgggact ggcctccgcg tcaccactac
     5701 atatgctgga aaaagtgttg accatgtttg acttacgcga cagtttcgat gccctcgcct
     5761 cggctgaaaa actgccttac agcaagccgc atccgcaagt atatctcgac tgcgcagcaa
     5821 aactgggcgt tgacccactg acctgcgtgg cgctggaaga ctcggtaaat ggcatgatcg
     5881 cttctaaagc agcccgcatg cgttccatcg tcgttcctgc gccagaagcg caaaatgatc
     5941 cacgttttgt attagcaaac gtcaaactct catcgctgac agaactcacc gcaaaagacc
     6001 ttctcggtta atgaccaggg gcagtgatca cgctgcccct ggttctttat ctgaatttgc
     6061 attcaactga cggattaatc gtcaatttaa gagaaagagt tacaccgtca ccacttccgt
     6121 gcactgtata aaaatcctat actgtacgta tcgacagttt agtgagtttt atcatgacgg
     6181 cggaaggtca ccttctcttt tctattgctt gtgcggtatt tgccaaaaat gccgagctga
     6241 cgcccgtgct ggcacagggt gactggtggc atattgtccc ttccgcaatc ctgacgtgtt
     6301 tgttaccgga catcgatcac ccaaagtcgt ttcttgggca gcgattaaaa tggatatcaa
     6361 aaccgatcgc ccgcgctttt gggcatcgtg gttttaccca cagtctgctg gcggtatttg
     6421 cgctgctggc aaccttttac cttaaggttc cggaaggctg gttcattccg gctgatgcgc
     6481 tacaaggaat ggtgctgggt tatttgagcc acatacttgc cgatatgctg acacccgccg
     6541 gtgttcccct gctctggcca tgccgctggc gtttccgctt gcctatcctg gttccccaaa
     6601 agggcaacca actggaacgt tttatctgca tggcattatt tgtctggtcg gtatggatgc
     6661 cccattcatt acccgagaac agcgctgttc gttggtcatc gcaaatgatc aataccttgc
     6721 agatccagtt tcatcggctt attaagcatc aggttgaata ctaaaaaggc aaaaatcacc
     6781 tttctggaat aagcaattcc atttgaatat aagagccagc tcacagttct gttaatcttg
     6841 cgccaacact atgactgcta cgcagtgata gaaataataa gatcaggaga acggggatga
     6901 actttccatt aattgcgaac atcgtggtgt tcgttgtact gctgtttgcg ctggctcaga
     6961 cccgccataa acagtggagt ctggcgaaaa aagtgctggt gggtctggtg atgggtgtgg
     7021 tttttggcct tgccctgcat accatttatg gttctgacag ccaggtactt aaagattctg
     7081 tacagtggtt taacatcgtt ggtaacggct atgttcaact gctgcaaatg atcgttatgc
     7141 cgttagtctt cgcctctatt ctgagcgcgg ttgcccgtct gcataacgca tctcagttag
     7201 gcaaaatcag ttttctgacc atcggtacgc ttttgtttac cacgctgatt gcggcgctgg
     7261 tcggtgtgct ggtcaccaac ctgtttggtt tgacggctga aggtctggtt cagggtggtg
     7321 cagaaactgc acgtctgaac gccattgaaa gtaactatgt tggtaaagtc tctgatcaga
     7381 gcgttccgca gctggtcttg tcctttatcc cgaaaaaccc gtttgccgat cttaccggag
     7441 ccaatccgac gtcaattatc agcgtggtaa tttttgccgc attcctcggc gtagctgcat
     7501 tgaaactgct gaaggatgat gcgccgaaag gggaacgcgt cttaaccgct atcgataccc
     7561 tacaaagctg ggtgatgaaa ctggttcgcc ttgtcatgca gttgacccct tacggcgttc
     7621 tggctctaat gaccaaagtg gttgcaggtt ctaacttgca agacatcatc aaactgggaa
     7681 gtttcgttgt cgcgtcctac ctcggtctgc tgattatgtt tgctgtgcat ggcattctgc
     7741 tgggcattaa tggcgtgagt ccgctgaagt acttccgtaa ggtatggcct gtgctgacgt
     7801 ttgcctttac cagccgttcc agtgctgcgt ctatcccact gaatgtggaa gcacaaacgc
     7861 gtcgtctggg cgttcctgaa tccatcgcca gtttcgccgc ctctttcggt gcaaccattg
     7921 gtcagaacgg ctgcgccggt ttgtatccgg caatgctgcg gtgatggttg cgcctacggt
     7981 tggcattaac ccgctggacc cgatgtggat tgcgacgctg gtcggtattg ttaccgttag
     8041 ttccgcaggc gttgccggtg tcggtggtgg tgcaactttc gccgcactga ttgtgctgcc
     8101 tgcgatgggc ctgccagtaa ccctggtggc gctgttaatc tccgttgaac cgcttatcga
     8161 catgggccgt acggcgctaa acgttagtgg ctcgatgaca gctggcacgc tgaccagcca
     8221 gtggctgaag caaaccgata aagccattct ggatagcgaa gacgacgccg aactggcaca
     8281 ccgttaattc ttatgctggc aacggtccgt tttgtatagg ggccgttgct ttacttttta
     8341 aatgttccat cgcggcgatg catactgact catattt
//
LOCUS       DAJTSI010000166         8330 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000166 DAJTSI010000000
VERSION     DAJTSI010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.166"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..157
                     /locus_tag="OO327_003501"
     CDS             <1..157
                     /locus_tag="OO327_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019912.1"
                     /translation="SESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGED
                     VEPGDDF"
     gene            complement(166..564)
                     /gene="vapC"
                     /locus_tag="OO327_003502"
     CDS             complement(166..564)
                     /gene="vapC"
                     /locus_tag="OO327_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCR6019913.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(564..791)
                     /gene="vapB"
                     /locus_tag="OO327_003503"
     CDS             complement(564..791)
                     /gene="vapB"
                     /locus_tag="OO327_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCR6019914.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            873..1244
                     /locus_tag="OO327_003504"
     CDS             873..1244
                     /locus_tag="OO327_003504"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCR6019915.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEASAST"
     gene            1265..6126
                     /gene="traI"
                     /locus_tag="OO327_003505"
                     /pseudo
     CDS             1265..6126
                     /gene="traI"
                     /locus_tag="OO327_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6146..6892
                     /gene="traX"
                     /locus_tag="OO327_003506"
     CDS             6146..6892
                     /gene="traX"
                     /locus_tag="OO327_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCR6019916.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6947..7507
                     /locus_tag="OO327_003507"
     CDS             6947..7507
                     /locus_tag="OO327_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR6019917.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7638..7850
                     /locus_tag="OO327_003508"
     CDS             7638..7850
                     /locus_tag="OO327_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR6019918.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8094..>8330
                     /locus_tag="OO327_003509"
     CDS             8094..>8330
                     /locus_tag="OO327_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR6019919.1"
                     /translation="MRKYIPLALFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1912 a         2086 c         2671 g         1661 t
ORIGIN      
        1 cagtgaatcc ggtgaagtgg tggatatggc cgcttatgag gcatggcagc aggaaaatca
       61 tccggacacc tggcagcaga tgcagcgtcg tgaagaggtg aacatcaatg tgcaccggga
      121 acgcggggag gatgttgagc cgggagatga tttctgatga acaggtcagc tccagtcttc
      181 agttctcagg ccgcccacac gttcaaattc ccgggtgtta ttagtcacaa taatcagccc
      241 ccgactgcgg gcatgacctg cgatcatttg atcaaatggc ccgacagggc gtccctgacg
      301 ggcaagttct gctcttatct ggccggtgtg tgtggctgca gcagcgtcgt aatccagaac
      361 atcaatgcgg gaaacaaatc cctcgatcac agcgagatta cgttcaggca tctggctttt
      421 ttctgcacca tatatcagct ccatcagggt gaccgaactg atacacattt tcccctggtt
      481 caggttaaaa cgctccctga cgctggcggg tttgttcttt atcgtaaaaa tgcagatgtt
      541 ggtatcgagc ataaacttca gcatcagaat gactcccttt cttgcatgcc gggctgttcc
      601 ctgttatcca taaaatcggt gctgacgctg tgtccgtcga accattcgtc ccatgtctct
      661 ccggctggcg taatgattct ggttcgtccg acagcaatca cttcaacgcg ttttacgttt
      721 tccggcaatg caaccgcttt tggcagtctg accgcctggc tgcggttgct gagaaatacg
      781 gtggtttcca ttatttgcct ccttatgtgg atatgtcttg tgtatatcta ttgtgggatt
      841 gataccggga tatgtcaaag ggatatacgt ttatgatgag tatcgcgcag gtcagatcgg
      901 ccggaagtgc cggtaattat tataccgaca aggataatta ctatgtgctg ggcagcatgg
      961 gagaacgctg ggccggccag ggggctgaac aactggggct gcagggcagt gtcgataagg
     1021 atgtctttac ccgccttctg gagggcaggc tgccggacgg ggcggattta agccgcatgc
     1081 aggatggcag taacaggcat cgtcccggct acgacctgac cttctccgct cccaaaagta
     1141 tctccatgat ggccatgctc gggggggaca aacgtcttat tgaagcacat aaccaggccg
     1201 tggattttgc tgtccgtcag gtggaggcca gtgcctccac ctgacggacg gacagtcaga
     1261 aacggtgctg accggtaatc tggtgatggc actgtttaac cacgacacca gtcgcgatca
     1321 ggaaccacag ttacacacgc atgcggtggt gactaatgtt acgcagtata acggcgaatg
     1381 gaagacactg agcagtgaca aagtggggaa aacggggttc attgagaatg tgtacgctaa
     1441 tcagattgcc tttggcaggc tctaccggga aaaactgaaa gagcaggttg aggcgctggg
     1501 ctatgaaact gaagtggtcg gtaagcacgg tatgtgggaa atgccgggtg taccggtgga
     1561 ggccttttcc ggacgctcac agactatccg ggaggccgtc ggggaagacg cctcgctgaa
     1621 atcccgggat gtggcggcgc tggatacgcg taaatccaaa cagcacgtcg atcctgaagt
     1681 cagaatggcc gagtggatgc agacgctgaa ggaaaccggg ttcgacatcc gggcatatcg
     1741 tgacgcagcg gaacaacggg catatacccg cacgcagaca cccggacccg cttcacagga
     1801 cgggccggat gtgcagcagg cggtgacaca ggccattgcc ggattaagtg aacgcaaagt
     1861 gcagtttatg tatacggacc tgctggccag gacggtcggc atactgccgc cggaaaatgg
     1921 tgtgattgaa cgggcacgcg ccggtatcga tgaggccatc agccgtgagc agcttatccc
     1981 cctcgatcgt gagaaggggc tgttcacgtt cgggattcat atgctcgatg agctgtcagt
     2041 ccgggcactc agtcgtgaca tcatgaaaca gaaccgggtg accgtacatc cggagaaaag
     2101 tgtcccccgg acggccggtt acagcgatgc cgtcagtgtt ctggcacagg accgtccgtc
     2161 gctggccatt gtgtccgggc agggcggtgc agccgggcag cgtgagcggg tggctgaact
     2221 ggtcatgatg gcccgggagc aggggcggga ggtgcagatt atcgctgctg accgtcgctc
     2281 gcagatgaac ctgaagcagg atgaacggct gtccggtgaa ctgataaccg gacgtcgtca
     2341 gttgctggaa ggaatggcct tcacgccggg cagtaccgtt atcgtcgacc agggcgaaaa
     2401 actctccctg aaagagacgt taaccctgct ggatggtgcc gcacgtcata acgtacaggt
     2461 cctgataacc gacagcgggc agcgaaccgg tacaggcagt gcactgatgg ccatgaagga
     2521 tgccggggtg aacacatatc gctggcaggg gggagaacag cgaccggcca ccatcatcag
     2581 tgaaccggac cgtaatgtcg gctggccgga gattttgcgg ccagcgtgaa agccggagaa
     2641 gagagcgtgg cacaggtcag cggagtacgg gaacaggcca tactgacaca ggccattcgc
     2701 agtgagctga aaacacaggg cgtgctcgga cacccggagg taaccatgac cgccctgtca
     2761 ccggtctggc tggacagccg gagccgttat ctgcgggata tgtaccgccc gggaatggtg
     2821 atggagcagt ggaacccgga gacacgcagt catgaccgct atgtgacaga acgtgtgacg
     2881 gcgcagagtc acagcctgac cctgcggaat gcgcagggcg aaacgcaggt ggtgcgtatt
     2941 tcttccctgg acagcagctg gtcgctgttc cggccggaaa aaatgccggt ggcagacggt
     3001 gagcgtctga gggtgacagg gaaaattccc ggactccgcg tctccggcgg tgaccgcctg
     3061 caggtggcat ccgtcagtga agatgcgatg acggttgttg tgccgggacg ggctgaaccg
     3121 gccaccctgc ctgtgagcga ttcaccgttc actgcactga agctggagaa cggctgggtg
     3181 gaaacgcccg ggcattccgt cagcgacagt gcgacggttt ttgcctccgt cacacagatg
     3241 gcaatggaca atgccaccct gaatggtctg gcccgcagtg gtcgtgatgt ccggctgtat
     3301 tcctcactgg atgaaacccg tactgcggaa aaacttgccc gccatccctc ctttacggtg
     3361 gtttctgagc agataaaggc gcgtgccggt gagacattgc tggaaaccgc tatcagtctg
     3421 cagaaagccg ggcttcacac gccggcacag caggccattc atctggccct tcctgtgctg
     3481 gaaagtaaaa acctggcctt cagcatggtg gacctgctga cagaggcgaa gtcgtttgct
     3541 gcagaaggaa ccggttttgc tgacctggga ggggaaatca atgcgcagat aaaacggggt
     3601 gatttactgt atgtggatgt ggcaaaaggc tatggcacag gcctgctggt ttcccgtgcg
     3661 tcgtatgagg cagaaaagag cattcttcgc catattctcg aaggtaagga ggcggtcacg
     3721 ccgctgatgg agagagtacc tggcgaactc atggagaaac tgacatcagg acagcgcgcc
     3781 gccacccgta tgatactgga aacgtctgac cgtttcacgg tggtgcaggg ctatgccggt
     3841 gtgggtaaga ccacacagtt ccgggcggtg atgtcagccg tgaacatgct gccggagagt
     3901 gaacgtcccc gtgtcgtggg gctggggccc acacaccgtg cggtcgggga gatgcgcagc
     3961 gccggcgtgg atgcacagac actggcgtcc tttctgcatg acacgcagct gcagcagcgc
     4021 agcggagaaa ctccggattt cagcaacacg ctgttccttc tcgatgagag ctctatggtg
     4081 ggcaataccg acatggcacg ggcatacgcc ctgattgcgg ccggtggcgg tcgggctgtg
     4141 gccagcggtg acacggacca gctgcaggcc atcgcgcccg gtcagccttt ccgtctccag
     4201 cagacgcgca gtgctgccga tgtggtcatc atgaaggaga ttgtgcgtca gacgccggaa
     4261 ctgcgggagg cggtatacag cctgattaac cgggatgtgg aaagggcgct gtccgggctt
     4321 gagcgtgtga aaccgtctca ggtgccacgt ctggagggcg catgggcacc ggagcactcc
     4381 gtgacggagt tcagtcacag ccaggaagcg aaactggcag aagcgcagca gaaggcgatg
     4441 ctgaaaggcg aggcttttcc ggatgtcccc atgacactgt atgaagccat tgtccgcgac
     4501 tataccggca ggacaccgga agcacgggag cagacgctga ttgtcacgca cctgaatgag
     4561 gaccggcgcg tactgaacag catgattcat gatgcacggg aaaaggccgg agaactgggg
     4621 aaggtgcagg tcatggtgcc tgtcctgaac acagcgaata tacgcgacgg ggagctgcgt
     4681 cgtctctcca cctgggagaa taacccggat gcccttgccc tggtggataa tgtgtatcac
     4741 cggattgccg gtatcagtaa ggatgacggg ctgataaccc tgcaggatgc ggaaggtaac
     4801 acgcggctga tttcgccccg ggaggcggtg gctgaaggcg tcacgctgta caccccggac
     4861 accatccggg tggggaccgg tgacaggata cgcttcacga agagtgaccg ggagcgcggt
     4921 tatgtggcca acagcgtctg gacggtgaca gcggtttccg gtgacagtgt cacactttct
     4981 gacggtcagc agacccgtgt gattcgcccc ggtcaggagc gggcagagca acatattgac
     5041 ctggcctatg ccatcaccgc ccacggtgca cagggggcga gtgaaacctt tgccatcgcg
     5101 cttgaaggca cggaaggtaa ccggaaactg atggccggct ttgagtcagc ctacgtggcc
     5161 ctgtcgcgta tgaagcagca tgtgcaggtg tacaccgata accgtcaggg ctggacggat
     5221 gccatcaaca atgccgtaca gaaaggaact gcccacgatg tgtttgagcc gaaaccggac
     5281 cgggaggtca tgaatgcaga gcggctgttc agtacggcgc gggagctgcg ggacgtggcg
     5341 gcaggccgtg ccgttctccg tcaggcgggg ctggccgggg gagacagtcc tgcacggttt
     5401 attgctccgg gacgtaaata tccgcaaccg tatgtggcac tgccggcgtt tgaccgtaac
     5461 ggcaagtccg ccggtatctg gctgaacccg ctgaccacgg atgacggaaa cgggctgcgg
     5521 ggattcagcg gtgaaggtcg ggtaaaaggc agcggggatg cgcagttcgt ggccctgcag
     5581 ggcagccgta acggagaaag cctgctggct gataatatgc aggatggcgt ccggattgcc
     5641 cgtgataatc ctgacagtgg tgtggtggta agaatcgccg gtgaaggccg tccgtggaat
     5701 cccggtgcca tcaccggtgg ccgcgtgtgg ggggatatcc cggacaacag cgtccagccg
     5761 ggagccggaa atggtgaacc ggtcacggca gaggtactgg cacagcggca ggctgaagag
     5821 gccatccgcc gtgaaacgga acgtcgcgca gatgaaattg tccgtaaaat ggcagagaac
     5881 aaacctgacc tgccggacgg caaaacagag caggctgtca gggaaattgc cgggcaggag
     5941 cgtgaccggg ctgccataac tgaacgggaa gccgcgctgc cggagagtgt actgcgtgaa
     6001 ccacaacggg tgcgggaagc ggtccgggaa gttgcccggg aaaatttgct gcaggagcgt
     6061 ctgcagcaga tggagcggga tatggttcgt gacctgcaga aagagaaaac cccgggcgga
     6121 gactgataca ggaagacaaa cactgatgac aacggataac acgaacacga cacgtaacga
     6181 ttcactggct gcacggaccg atacctggtt gcagtcattt ctggtctggt cacccggaca
     6241 gcgggatatc atcaaaacgg tggcactggt gctgatggta ctggaccaca tcaatctgat
     6301 attccagctg aagcaggaat ggatgtttct ggccgggcga ggagcctttc cgctttttgc
     6361 cctggtgtgg gggctgaatc tgtcccgtca tgcgcatatc cggcaaccgg ccattaaccg
     6421 gctgtgggga tggggaatta ttgcccagtt cgcatattat ctggcaggct ttccttggta
     6481 tgaggggaat atcctgtttg cctttgcagt ggcggcacag gtgctgacgt ggtgtgaaac
     6541 gcgcagtggg tggcgtacag cagcggccat actcctgatg gccctgtggg ggcctttgtc
     6601 cggcaccagt tacggtattg ccgggttgct gatgctggca gtcagcaacc ggctgtaccg
     6661 ggcggaagac agagcggaac gtctggcgct ggtcgcctgc ctgctggctg ttattccggc
     6721 gcttaacctt gccaccagtg atgcagcggc ggtggccggt cttgtgatga cggtgctgac
     6781 cgttggtctg gtgttgtgtg ccggaaaatc attaccccgt ttctggcccg gagacttttt
     6841 cccgacgttc tatgcctgtc atctggctgt gctgggcgtt ctggcgctgt gacgggtgtg
     6901 gtatctttgg ccgcaagagg atgattcgtc agaggcagaa cacagcatga cagagcagaa
     6961 gcgaccggta ctgacactga agcggaagac agagggaaca gcgcctgtcc gcagccggaa
     7021 aaccatcatc aatgtcacca cgccaccaaa gtggaaggtg aaaaagcaga agctggccga
     7081 gaaagcggcc cgtgaagcag agctggcggc aaaaaaagcg caggccagac aggcgctgtc
     7141 catttatctg aacctgccca cgctggatga ggctgtgaat accctgaagc cctggtggcc
     7201 gggattattt gacggtgaca cgccccggct tctggcctgc ggtatccggg acgtgttact
     7261 ggaagacgtg gcgcagcgga atatcccgct ctcgcataaa aaactgcgca gggcgctgaa
     7321 ggccatcacc cgttcagaaa gctatctgtg tgccatgaaa gccggtgcct gccggtatga
     7381 cacggaaggg tatgtgacgg agcatatttc tcaggaggag gaagcgtatg cgggagcgcg
     7441 tctggcgaaa attcgtcgcc agaaccggat aaaggcagaa cttcaggccg tgcttgatga
     7501 gaaataaaaa aagcctcccc cagagagagg aggcagggaa ataaggctta aaaggaaata
     7561 acttccgctt ccgaaaggaa gccagtccac tgaattattt atcacaataa tactgacaac
     7621 taaaggggga caagaaaatg aatggattca gaaacagttc acggaacggc caggtctggc
     7681 gttaccagcg tgccggcggg cgggccgtta ttctggaagt cagtggacgc tggatggaag
     7741 cggcagaagc atggcgacgg gctgcctgtg ttgcgcctag gacagactgg cagcagtttg
     7801 cccggaaaag ggctgaacac tgccaccggc gctgccgggg aagggtgtaa cgaaaaaagc
     7861 agccttcatt actctaggag tcagaaggct gaaaggagtt tatgagattt gcttcttaat
     7921 atccggtcgg gcagtatgac cggacagttc tgagtagcac agagtctttt ccgcattaag
     7981 tcgatttttt atattttgtg attagtttca caaaataagg tgttattcaa tgtgtgctgc
     8041 actattcagg ggggctgaaa tacaggtggc atttatctga tatggagaat aacatgagga
     8101 aatatattcc actggcatta tttatctttt catggccggt attgtctgca gatattcatg
     8161 gacgggttgt tcgtgtactg gacggtgata ccatcgaggt catggactca ctgaaggcgg
     8221 tgcggatcag gctggtaaat attgatgcac cggaaaagaa acaggattat ggccggtggt
     8281 caacagacat gatgaaatca ctggtcgcag gaaaaacggt taccgtcaca
//
LOCUS       DAJTSI010000167         8279 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000167 DAJTSI010000000
VERSION     DAJTSI010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8279
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.167"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            7..657
                     /locus_tag="OO327_003510"
     CDS             7..657
                     /locus_tag="OO327_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019920.1"
                     /translation="MHRIDTKTAKKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFPM
                     TFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            629..1063
                     /locus_tag="OO327_003511"
     CDS             629..1063
                     /locus_tag="OO327_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6019921.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(1836..2885)
                     /locus_tag="OO327_003512"
     CDS             complement(1836..2885)
                     /locus_tag="OO327_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR6019922.1"
                     /translation="MYNNKIDSNRKIRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAGFFTIKKNDLSVFDYIKSKFYRLMIPYFTFAFSILIINTINSGETI
                     DYIYNHIYDIIYGVRNNQFVGTIWFINCLFVIIAIDAIFREIVKNNIVILIISLLSFM
                     LSQTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKIGFIVFSI
                     TAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIFIAKIICG
                     NDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKI
                     GHWLSRTVGGPFLIK"
     gene            3336..4526
                     /locus_tag="OO327_003513"
     CDS             3336..4526
                     /locus_tag="OO327_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl9 family transposase"
                     /protein_id="HCR6019923.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGPARVKDKIKRVDCLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLGTGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
     gene            complement(4916..6376)
                     /gene="gtrII"
                     /locus_tag="OO327_003514"
     CDS             complement(4916..6376)
                     /gene="gtrII"
                     /locus_tag="OO327_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
                     /protein_id="HCR6019924.1"
                     /translation="MIKINLFKNANLLAFISCFAISIYCYWGWLYDGTLNIDGEFTNN
                     FYQTITLGRWFHTFLRHYFLPEPFSLYITPLIALSFIIISAFIICRSLKLESYELLIG
                     MLVFITFPQISYQLEFLNQADTVGIAFLLAAISAIIFHSQKNRIVIFSGIVLSILSMA
                     IYQTFVTYIIAFVIGLQINSIIRNEKNIRESFYSSCLSLSLIALSTLIYLLLTKAIKH
                     YFSLESNEYISNYIQNASDIKWLVKSAIDNIYNFYNNPPTGLNLYKWLLIPLLILMFT
                     LTYKLKTRSIYLISSIIFIYILPVIFIVVVGSGAPPRLFVLMPIVAVILFSCLSNFRS
                     IKYLNCMFFLFVIFNGVSTSKNLFLNDTLARQKDISLAKEISYTSQTKGISLNGKKIY
                     IYGSNDSGNMLSMSADTFGKSFFWWDGGNYFRMVAFMNYYGICNCKPANKEQIEKIYP
                     IVKSLPSWPNPDSIAEINGLVIIKLSEKKGWLPFNI"
     gene            complement(6373..7302)
                     /locus_tag="OO327_003515"
     CDS             complement(6373..7302)
                     /locus_tag="OO327_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR6019925.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGPTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYIEVKNRPKYIIKKSHRGNP"
     gene            complement(7299..7661)
                     /locus_tag="OO327_003516"
     CDS             complement(7299..7661)
                     /locus_tag="OO327_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR6019926.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKALTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            complement(7923..>8279)
                     /locus_tag="OO327_003517"
     CDS             complement(7923..>8279)
                     /locus_tag="OO327_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6019927.1"
                     /translation="VFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRF
                     HDLRHTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRN
                     GTNTAQEENVVYLNAR"
BASE COUNT         2528 a         1598 c         1686 g         2467 t
ORIGIN      
        1 taatccatgc atcgtataga cacgaaaacc gcgaagaagg ataagttcgg cgcgggtaag
       61 aacggtttta cccgtggtaa cccccagact ggcacgcctg ccaccgatct ggatgatgac
      121 tactttgaca tgttgcagga ggagctttgc agcgtggtgg aggcatccgg tgccagcctg
      181 gagaaggggc ggcacgatca gttgcttacc gcacttcgcg cgctgctgtt aagccgcaag
      241 aatccgtttg gcgatatcaa atcggatggc acggtgaaaa cggctctcga aaaccttggt
      301 ttgggagaag cagctaaaag gaatgtaggt acaggggcga atcagatacc tgatatgagc
      361 ctgttcgcgt caattaatac cgtaacggct gctgcgcaaa aatttccgtc tggattaatt
      421 ttacagtgcg gtcagttgaa tggtgccccg aatgtatctt caacatacgg gatgaggttc
      481 ccgatgacgt tctcaagagt aattgctgtc gtagttacat tgaacgttac tggcgcggct
      541 gggcagccga ctgtatcggc gacaagtgtc cagaacactg gatttaatat tactgtgtcg
      601 cccggttcag gatacggttc atctgctgat gcgtattaca ttgcaatggg atattaacaa
      661 aatgtcatat ttttattctg catcgacaaa cggattttat tcgactgaat ttcacggcac
      721 caatattcct gatgatgcag tggaaatctc ggaatcagag tggaaaacac tgattaatgc
      781 acagagtgta acaaaaatga ttacctgtgg tgagaacggt catcctgtca ttgttgaccg
      841 tccttctcca acaccagaac aattagcctt aataaatgat gaaaagaaat ctgcactgat
      901 agcagaggca acgaatgtaa tagctccgct tcaggatgcg gttgatttag gtatggcaac
      961 agatgatgaa acgaaactgt tactggcatg gaaaaaatat cgggtgctgc ttatgcgtgt
     1021 taatgtagta aaacccgagt ggcctatgca tccaaataaa tagacttgtt aatctaggat
     1081 aattgttgga ataatactcg tttttcagta ggttcaggta gcctgggtat aatgctcact
     1141 tctacaatga tttcaactta ttttatttaa ttgatattac gaaacttaac tcatacataa
     1201 acaatatgac tcaatactgt cctaaatagc tgcgcagaaa agtgctccac tttcgctcag
     1261 cccttgctgg gcgaagcatc aggaaggcgt tctcagctag gttgtgttat atctggcttg
     1321 agttttgttg cccatacgca acacattacc catctgtaaa cgacaagtgt ggcagaatta
     1381 ggtgcagcta tttagactat atcgacgcag taacgataat agatatcgaa actgtactag
     1441 atattagcta cttatggtta ccggggggag tcttttgagc actggtgtta tcaattggct
     1501 tcaaataatt cgccaataaa ttatattgcg atagctcatc accttccggg ttgtttatag
     1561 ccagtttatc agcgtcacca cctactgttt atgcttttat ttctttgatt aataagtata
     1621 tgatatttta attcatctgg tgtatttctt tctcatgtgt tggtcgactg aatatctgtt
     1681 cagtgaaggg gtaattttat gtttgcactt cgaacattcc cttcagttgc taggaaagaa
     1741 aaccgtaaca tttgcgcatt aattttaaca gtataatctg agcataaata tgcgctatat
     1801 atctataaaa aggcgcaatt aattgcgcct ttttatcatt ttatcaaaaa tggcccaccg
     1861 actgtcctcg atagccaatg gccaatttta tctgatacat atacacaaat acaggtatat
     1921 atgattgcgc agataggatt tggaatagtc acgctgaagc ctataattgc taaagccata
     1981 gggataaata atttggtgat atactccatt ccacatatgt taagagtatt tcttccagct
     2041 cttacaataa agtcatttcc acaaattatt tttgctatga aaatattaaa gattatcaag
     2101 cctaccgtgg tgataatagt attaatagct tgaatataac tacttgatat tatgtctgca
     2161 ttgaatattg agataatctt aaaaagtaaa ggtttctggt taagtagttg atacgcactc
     2221 attatagcgg taattgagaa gacaatgaaa ccaattttag attttccaaa aaacctgtca
     2281 cgtgttaatt ctaaaaacat acatctaccg agaggcaata accaccagta tgccatagcg
     2341 gagtctatgt tccaaaacca ttgaggatct agtaatgggt tatgattgag cactgtttgt
     2401 gacaacatga acgataacag tgatatgatt aaaatcacta tattgttctt gactatttct
     2461 ctaaagattg catcaattgc tattataaca aaaaggcagt ttataaacca tattgttcct
     2521 acgaattggt tatttctaac accatatatt atatcataaa tgtggttgta tatataatca
     2581 atcgtctcac ctgagttaat tgtgtttatt attaatatag aaaatgcaaa agtgaaatat
     2641 ggaatcatta atctgtaaaa ctttgacttg atataatcga aaacagataa gtcatttttt
     2701 ttgatagtaa aaaaaccagc agcgaaaaag aatagtggga cgtgataact aaaaacaaaa
     2761 ggatacaatt tgccagcccc caacccaagg tgacctaaat atatagcaaa aataccgaga
     2821 aattttaaag catcaaccca atcatgtctt atttttctgt tgctgtcgat tttgttatta
     2881 tacatttaaa gtaggctctc cagttttgtg tgatttttaa acactcattg caaatatgaa
     2941 tgcctgaaag tttatgatta ttaatcaacg agttcaatat taatttatta tttttcaaag
     3001 tcgcactata taggcattta cttccgcagt accaccgata gtatcgatat caaatcagcc
     3061 tgcggccgcg ataactttcg taacattctg ccagcggctt tgttgaataa atcaggttca
     3121 agataatcgt ctctgcatca ggttagagtt tcaggcaata catcctcttt ctggcatacc
     3181 tgccactctc gcaccatggt catagccttt acgatctgca tactcaccag accagagccc
     3241 gctccctttt aggctgagga atccccagaa aagaagacag gcatagagtt ttgtgatcta
     3301 ctgattgcgc taacaaattc aatgagatca cctctatgcc tgcccgtaaa gtatgccaga
     3361 cttttttccg taatgcttta gcttccacgc atcagtatcg acaaaatgcc attattgatt
     3421 ctgctgctgc tttagtcggt ggcgcatctc tttctcttac cagcattggc cggcatttac
     3481 ccggtcctgc tcgcgtgaaa gataaaataa aacgcgttga ctgtctgttg ggtaataaac
     3541 gacttcataa tgatatccct ctgatattca aaaatattac atccatgatg acaaataaac
     3601 tgtcgtggtg tgttatcgct gttgactgga gcggttaccc ttttcaggaa tatcatgtcc
     3661 ttcgtgccag cctcctctgc gacggtcgct ctatccccct gatgagtcag gtcttcccgt
     3721 caaaaaaaaa taacgaggcg gtggaaatcg cttttctgga tgcgctttcc ggggccattt
     3781 ctccccggac tcgtgttgtt attgttactg atgctggttt tcaaagtgca tggttccgtc
     3841 atatcaaatc acagggatgg gattttattg gtcgtatcag gggggtagtg aagttcaggc
     3901 tggatagtga caaagataag tggctggaca taaaaatgtg cagggggagt tcagaggcaa
     3961 aatacctggg gacgggaacc ctggcccgga aaaaacgctc gcagtgtgaa ggacattttt
     4021 atctttataa acattcacct aaaggcagaa aaagcaggcg tgccagaggg aggccgggct
     4081 taccaacaac ggaaaaagaa caaaaagccg caggcagaga accatggtta atattcagca
     4141 atacagctga gtttaatgcc aaaaaaatca tgaaactgta cagtcgaaga atgcagatag
     4201 agcaaaactt tcgggatgaa aaaagtgaac gctttggttt tggccttcgg gcgagcagaa
     4261 gtcggagggg agagcgtttt ctggtattaa gtctactggt tacactggcc agtatcgtac
     4321 tctggctact tggttatcat tttgaaaata aaggatttca tttaaagtat caggctaata
     4381 gtctcaaaaa acgaagagta ttgtcttttc tgacgctggc tgaaaacgtc ctgagattta
     4441 accctgaact tctacgacga gcacaacctg aaaagatatt gtgccggttg gccagcactt
     4501 accgaagtat ggtgctggct tactgatgaa aattttctgg ggatccctca gcctttcggg
     4561 gaggaatgag ggctctgata ttcttttgcc gtagttcatc gtgaaaccgt cggatgtcat
     4621 ttggcctgag tgacccatgc ctcccaaaag cagagtcgct agtgttatgt aattaggcgg
     4681 ttaaacttca attctcagtg ttaagtttgg gtaaacgctt gtaaatggta ttgctaattt
     4741 tttaattgac taatattaga aacggttagt gtgattgtat atagatattt gagtgtaagg
     4801 tatttaaagg ggcattcagc ccctccgtct atttgtgatt gcatccattg atgtgtgtat
     4861 tattttgagc ttgaaaaagg gaggcgctct ttaataataa ataaatctat caaagctaaa
     4921 tattaaatgg aagccaccct tttttctctg atagttttat tatcaccaaa ccatttattt
     4981 cagctatcga atctggattc ggccaggaag gtaaactctt cacaattgga taaatcttct
     5041 ctatttgttc tttatttgct ggtttgcaat tacagattcc atagtaattc ataaatgcaa
     5101 ccatcctaaa atagttgcca ccatcccacc aaaaaaaaga ttttccaaaa gtgtctgcgc
     5161 tcatggagag catatttcct gagtcgtttg aaccatatat atatattttt tttccgttaa
     5221 gggaaatgcc ttttgtttga gatgtgtatg atatttcttt agctaaagag atatctttct
     5281 gtcttgcgag agtatcattc aaaaatagat ttttggatgt tgaaacgcca ttaaatataa
     5341 caaataaaaa aaacatgcag tttaggtatt ttatagagcg aaaattgctt aagcaagaaa
     5401 acaaaattac tgctactata ggcattaaaa caaacaggcg aggtggcgcc cctgagccaa
     5461 caacaacgat aaatataacc ggaagtatat aaatgaaaat gattgatgaa atcaaataaa
     5521 ttgatcttgt ttttaattta tatgttaggg taaacatcag aattaataaa ggaatcagta
     5581 accacttgta taggtttaaa ccagtgggag gattgttata gaagttatat atattatcta
     5641 tggctgattt aacaagccat ttaatatcgc ttgcattttg tatataattt gagatgtact
     5701 cgttcgattc aagcgaaaaa taatgcttga tagctttggt taatagcagg taaattaagg
     5761 tagataaagc tatgagggat agagataaac atgaactata aaaagattca cgaatatttt
     5821 tctcatttcg tattatcgaa tttatctgca acccaatgac gaatgcaata atatatgtta
     5881 cgaatgtttg gtagattgcc attgaaagaa tggacagtac tataccagaa aatatcacaa
     5941 tcctattttt ttgcgagtga aaaataattg ctgatatcgc tgccagtaga aaagcaattc
     6001 ccacagtatc agcttggtta agaaactcta attgatagga gatctgaggg aaggtaataa
     6061 atactaacat acctatcaat aattcataag attctagctt tagcgatctg cagattataa
     6121 atgctgaaat gatgataaaa gataaggcta ttaatggtgt tatataaagt gaaaaaggct
     6181 cagggagaaa gtaatgtcgc aaaaaagtgt gaaaccaccg cccaagcgtg attgtttgat
     6241 aaaaattatt tgtaaactcg ccatcaatat taagtgttcc atcgtacaac cacccccaat
     6301 aacaataaat tgatattgca aaacatgata tgaaggcaag gagatttgca tttttaaata
     6361 aattaatttt aatcatgggt tacctcgatg agattttttt atgatgtatt tgggtctatt
     6421 ttttacttca atatagattc tacctatata ttctccgaga acaccaatcc cgatcagttg
     6481 cactccaccc aagaaaagta ttgatacaag cagggaggga tacccgcgta ctgggttacc
     6541 aaagacaagg gtgtctataa tcatccatgc accatataaa aatgaaatgc ttgcaacaaa
     6601 caagcctata taagtccata cgcgaagagg aaaggttgaa aaacttgtga taccttccag
     6661 tgccagattc cataatttcc agccattaaa ttttgagatg ccagcaacac gctctgcgcg
     6721 tacatattca acgacatccg tcggaccacc cacccagctc agtatgcctt tcatgaaaag
     6781 attgcgctca ggcaacagtt taatgttctc cacaacctca cgagacatga gtcgaaaatc
     6841 tccgacattt tcctcgatct ttggggtgct tattttgttg tgtaatttat agaaccactc
     6901 agctgtctta cgtttcagtc gtccatcagt tgagcggtct gaacgtttag caagcaccat
     6961 gtcagcacct gcctgccact tttcaataag atgagggata acctcaattg ggtcttgcag
     7021 gtcgacatca ataggaatta cagcatcgcc gcttgcatgg tctaaccctg caaataaggc
     7081 tggttcttta ccaaagttgc gtgtaaatga cagcggaaca actagcgggt ctgaaacagc
     7141 cagcgcgtta ataattgact ctgtggcatc tttacttccg tcatttatga atacaatttc
     7201 tacttcatat ggcttcaact cttggaattc acgtaccgtt ttatagaaaa caggtatcgc
     7261 ttcttcttca ttgaagacag gaacgacaag agatattttc atttcgcatc cctaaagaca
     7321 atgaactttg aatagacgaa accgcacacc agactgatgg cggagaaggt gataagagtg
     7381 actattggag gaagtgagca tttatcagct gcccaaccaa caatcacact caatattccc
     7441 ataaatccca cgtataacat gtagcgcatc gctgtagtca atgctttgaa tgtgaatctt
     7501 gcattcgcga agaagctaaa gctcacagcc actacgaaac ctgtgaagtt tgccagagcc
     7561 tgactggtat gtgcggcata gatacataca ccaaaaacca cccagtgtat aagtgtgttc
     7621 agcacaccta tagatgtgta ctttacaaat aactttaaca tttatttaat caatgagctc
     7681 tgaaaggcat gaagtctatc atccaagtct caattgatcg atacttgcta tgtctgatga
     7741 gacaaaactg agacacataa ggcctcacaa tggcttgcaa ggctttacat gttttgatgt
     7801 ggtgggacgt gtgagcgcag tgttgatggg ataatccttt gaattacaag cggattctta
     7861 taattcgtaa tgcgaaggtc gtaggttcga ctcctattat cggcaccatt taaatcaata
     7921 agttacctcg catttaagta aactacgttc tcctcttgtg ccgtatttgt gccattgcga
     7981 cttataatcg catcgatttt gctcgcgtgc tcggtaagat gcccggctga aaggtgggcg
     8041 tatctttgaa ccatttcgag agtttcccat cctcccatct ctttaagcgc aagaagagag
     8101 acaccggact gaaccagcca gcttgcccag gtatgcctca ggtcatggaa gcggaagttg
     8161 ctaatgcctg cccgctttaa cgctcccttc catgccttgt tgctgtcggt tctcatcttc
     8221 cttaccgctg ctgtttttgt tccgtcgctt cggtaggcag gtttggtgtg gacaaacac
//
LOCUS       DAJTSI010000168         7909 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000168 DAJTSI010000000
VERSION     DAJTSI010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7909
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.168"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(123..953)
                     /locus_tag="OO327_003518"
     CDS             complement(123..953)
                     /locus_tag="OO327_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR6019928.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            1163..2176
                     /gene="intI1"
                     /locus_tag="OO327_003519"
     CDS             1163..2176
                     /gene="intI1"
                     /locus_tag="OO327_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR6019929.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2379..2729
                     /locus_tag="OO327_003520"
     CDS             2379..2729
                     /locus_tag="OO327_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR6019930.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            2855..3415
                     /locus_tag="OO327_003521"
     CDS             2855..3415
                     /locus_tag="OO327_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6019931.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            3418..6384
                     /locus_tag="OO327_003522"
     CDS             3418..6384
                     /locus_tag="OO327_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR6019932.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            6451..6828
                     /locus_tag="OO327_003523"
     CDS             6451..6828
                     /locus_tag="OO327_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6019933.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7029..7688)
                     /locus_tag="OO327_003524"
     CDS             complement(7029..7688)
                     /locus_tag="OO327_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR6019934.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1753 a         2241 c         2133 g         1782 t
ORIGIN      
        1 cttccctcat gatgtttaac gtttgaaata aggggcgccg agcgccagcg aggggagcca
       61 aaagcttgct tttggccgtc ccgacttgat tgaagggttg ggcgattttg ccattagatt
      121 ttttataaat ttagtgtgtt tagaatggtg atcgcatttt tcttggcttt tatgcttgat
      181 gttaaattcg accccaagtt tcctgtaagt gcggacacaa aaacatattt atgtcctgat
      241 ttgcttataa taaacccttc aaaccatccg ttttgtaagg ttctatttgc tgtgaatcct
      301 gcaccagttt tcccatacag ttttgtacta ttatccagat cttgtagata catgttctct
      361 atggtgtttt ctatggctga gtttttaact gggagattgt gattaataat tttacgcagg
      421 aattgaattt gttcttctgg tgaaattttt aagctacttt cgagccatgc ttctgttaat
      481 ccgttgtttc tttctttatc tccagagaag tcttgatttc cataatcaaa atctttgaga
      541 taattcttga ttttatttaa tccaattttt tgggttattt cttgcgaaac ccaaacaaca
      601 gaaaattgca tccacgtctt tggtgtatga ttgctgttcc agatctccat tcctttgggg
      661 gttttatccc atttgaatat ggttttctga tctattattt ccgcatcaaa tgccataagt
      721 gataatgcga tcttgaaagt tgaatctggt gccatttgcg ttgcacactt tgctttattg
      781 aattgagcaa tttcagcgtt tgtggatgca tcgtaaagta aaaaacaacc ttcagttcct
      841 tcaaataatg gagatgcaac agtagagata tctgttgatg cactggcgct gctgtagata
      901 atatttgcaa ttattaaaaa aatagcgaag ttgatatgta ttgtgttttt cataataagt
      961 attggtttgg taaagggctt aattttaacg gctaacaatt aatgaggctc cgggttcgcc
     1021 caactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa
     1081 acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacccc
     1141 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
     1201 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gcttacgaac
     1261 cgaacaggct tatgtccact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc
     1321 ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc tggctggcga acgagcgcaa
     1381 ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg ctgttcttct acggcaaggt
     1441 gctgtgcacg gatctgccct ggcttcagga gatcggaaga cctcggccgt cgcggcgctt
     1501 gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc ggttttctgg aaggcgagca
     1561 tcgtttgttc gcccagcttc tgtatggaac gggcatgcgg atcagtgagg gtttgcaact
     1621 gcgggtcaag gatctggatt tcgatcacgg cacgatcatc gtgcgggagg gcaagggctc
     1681 caaggatcgg gccttgatgt tacccgagag cttggcaccc agcctgcgcg agcagctgtc
     1741 gcgtgcacgg gcatggtggc tgaaggacca ggccgagggc cgcagcggcg ttgcgcttcc
     1801 cgacgccctt gagcggaagt atccgcgcgc cgggcattcc tggccgtggt tctgggtttt
     1861 tgcgcagcac acgcattcga ccgatccacg gagcggtgtc gtgcgtcgcc atcacatgta
     1921 tgaccagacc tttcagcgcg ccttcaaacg tgccgtagaa caagcaggca tcacgaagcc
     1981 cgccacaccg cacaccctcc gccactcgtt cgcgacggcc ttgctccgca gcggttacga
     2041 cattcgaacc gtgcaggatc tgctcggcca ttccgacgtc tctacgacga tgatttacac
     2101 gcatgtgctg aaagttggcg gtgccggagt gcgctcaccg cttgatgcgc tgccgcccct
     2161 cactagtgag aggtagggca gcgcaagtca atcctggcgg attcactacc cctgcgcgaa
     2221 ggccatcggt gccgcatcga acggccggtt gcggaaagtc ctccctgcgt ccgctgatgg
     2281 ccggcagcag cccgtcgttg cctgatggat ccaacccctc cgctgctata gtgcagtcgg
     2341 cttctgacgt tcagtgcagc cgtcttctga aaacgacaat ggaggtggta gccgagggtg
     2401 tggaaacacc cgactgcctt gcgtggttgc ggcaggcggg ttgcgacacg gtgcagggtt
     2461 tcctgttcgc caggccgatg ccggcggcgg ccttcgtcgg cttcgtcaac caatggagga
     2521 acaccaccat gaacgccaat gaaccgagca ccagttgctg cgtgtgctgc aaggaaatcc
     2581 cgctcgatgc cgccttcacg ccggaagggg ccgagtacgt ggagcatttc tgcgggctgg
     2641 agtgctatca gcgcttccag gcgcgggcca gcactgcgac cgaaaccagc gtcaaaccgg
     2701 acgcttgtga ttcgccgccg tcaggttgag gcatacccta acctgatgtc agatgccatg
     2761 tgtaaattgc gtcaggatag gattgaattt tgaatttatt gacatatctc gttgaaggtc
     2821 atagagtctt ccctgacatt ttgcagggaa ttccatgact ggacagcgca ttgggtatat
     2881 cagggtcagc accttcgacc agaacccgga acggcaactg gaaggcgtca aggttgatcg
     2941 cgcttttagc gacaaggcat ccggcaagga tgtcaagcgt ccgcaactgg aagcgctgat
     3001 aagcttcgcc cgcaccggcg acaccgtggt ggtgcatagc atggatcgcc tggcgcgcaa
     3061 tctcgatgat ttgcgccgga tcgtgcaaac gctgacacaa cgcggcgtgc atatcgaatt
     3121 cgtcaaggaa cacctcagtt ttactggcga agactctccg atggcgaacc tgatgctctc
     3181 ggtgatgggc gcgttcgccg agttcgagcg cgccctgatc cgcgagcgtc agcgcgaggg
     3241 tattgcgctc gccaagcaac gcggggctta ccgtggcagg aagaaatccc tgtcgtctga
     3301 gcgtattgcc gaactgcgcc aacgtgtcga ggctggcgag caaaagacca agcttgctcg
     3361 tgaattcgga atcagtcgcg aaaccctgta tcaatacttg agaacggatc agtaaatatg
     3421 ccacgtcgtt ccatcctgtc cgccgccgag cgggaaagcc tgctggcgtt gccggactcc
     3481 aaggacgacc tgatccgaca ttacacattc aacgataccg acctctcgat catccgacag
     3541 cggcgcgggc cagccaatcg gctgggcttc gcggtgcagc tctgttacct gcgctttccc
     3601 ggcgtcatcc tgggcgtcga tgaactaccg ttcccgccct tgttgaagct ggtcgccgac
     3661 cagctcaagg tcggcgtcga aagctggaac gagtacggcc agcgggagca gacccggcgc
     3721 gagcacctga gcgagctgca aaccgtgttc ggtttccggc ccttcaccat gagccattac
     3781 cggcaggccg tccagatgct gaccgagctg gcgatgcaaa ccgacaaagg catcgtgctg
     3841 gccagcgcct tgatcgggca cctgcggcgg cagtcggtca ttctgcccgc cctcaacgcc
     3901 gtcgagcggg cgagtgccga ggcgatcacc cgtgctaacc ggcgcatcta cgacgccttg
     3961 gccgaaccac tggcggacgc gcatcgccgc cgcctcgacg atctgctcaa gcgccgggac
     4021 aacggcaaga cgacctggtt ggcttggttg cgccagtctc cggccaagcc aaattcgcgg
     4081 catatgctgg aacacatcga acgcctcaag gcatggcagg cactcgatct gcctaccggc
     4141 atcgagcggc tggttcacca gaaccgcctg ctcaagattg cccgcgaggg cggccagatg
     4201 acacccgccg acctggccaa attcgagccg caacggcgct acgccactct cgtggcgctg
     4261 gccaccgagg gcatggccac cgtcaccgac gaaatcatcg acctgcacga ccgcatcctg
     4321 ggtaagctgt ttaacgctgc caagaataag catcagcagc agttccaggc gtcaggcaag
     4381 gccatcaacg ccaaggtacg tctgtacggg cgcatcggtc aggcgctgat cgacgccaag
     4441 caatcaggcc gcgatgcgtt tgccgccatc gaggccgtca tgtcctggga ttcctttgcc
     4501 gagagcgtca ccgaggcgca gaagctcgcg caacccgatg acttcgattt cctgcatcgc
     4561 atcggcgaga gctacgccac cctgcgccgc tatgcaccgg aattccttgc cgtgctcaag
     4621 ctgcgggccg cgcccgccgc caaaaacgtg cttgatgcca ttgaggtgct gcgcggcatg
     4681 aacaccgaca acgcccgcaa gctgccagcc gatgcaccga ccggcttcat caagccgcgc
     4741 tggcagaaac tggtgatgac cgacgccggc atcgaccggc gctactacga actgtgcgcg
     4801 ctgtccgagt tgaagaactc cctgcgctcg ggcgacatct gggtgcaggg ttcacgccag
     4861 ttcaaggact tcgaggacta cctggtaccg cccgagaagt tcaccagcct caagcagtcc
     4921 agcgaattgc cgctggccgt ggccaccgac tgcgaacaat atctgcatga gcggctgacg
     4981 ctgctggaag cacaacttgc caccgtcaac cgcatggcgg cagccaacga cctgccggat
     5041 gccatcatca ccgagtcggg cttgaagatc acgccgctgg atgcggcggt gcccgacacc
     5101 gcgcaggcgc tgatagacca gacagccatg gtcctgccgc acgtcaagat caccgaactg
     5161 ctgctcgaag tcgatgagtg gacgggcttc acccggcact tcacgcactt gaaatcgggc
     5221 gatctggcca aggacaagaa cctgttgttg accacgatcc tggccgacgc gatcaacctg
     5281 ggcctgacca agatggccga gtcctgcccc ggcacgacct acgcgaagct cgcttggctg
     5341 caagcctggc atacccgcga cgaaacgtac tcgacagcgt tggctgaact ggtcaacgct
     5401 cagtttcggc atccctttgc cgggcactgg ggcgatggca ccacatcatc atcggacgga
     5461 cagaatttcc gaaccgctag caaggcaaag agcacggggc acatcaaccc aaaatatggc
     5521 agcagcccag gacggacttt ctacacccac atctccgacc aatacgcgcc attccacacc
     5581 aaggtggtca atgtcggcct gcgcgactca acctacgtgc tcgacggcct gctgtaccac
     5641 gaatccgacc tgcggatcga ggagcactac accgacacgg cgggcttcac cgatcacgtc
     5701 ttcgccctga tgcacctctt gggcttccgc ttcgcgccgc gcatccgcga cctgggcgac
     5761 accaagctct acatcccgaa gggcgatgcc gcctatgacg cgctcaagcc gatgatcggc
     5821 ggcacgctca acatcaagca cgtccgcgcc cattgggacg aaatcctgcg gctggccacc
     5881 tcgatcaagc agggcacggt gacggcctcg ctgatgctca ggaaactcgg cagctacccg
     5941 cgccagaacg gcttggccgt cgcgctgcgc gagttgggcc gcatcgagcg cacgctgttc
     6001 atcctcgact ggctgcaaag cgtcgagcta cgccgccgcg tgcatgccgg gctgaacaag
     6061 ggcgaggcgc gcaatgcgct ggcccgtgcc gtgttcttca accgccttgg tgaaatccgt
     6121 gaccgcagtt tcgagcagca gcgctaccgg gccagcggcc tcaacctggt gacggcggcc
     6181 atcgtgctgt ggaacacggt ctacctggag cgtgcggcgc atgcgttgcg cggcaatggt
     6241 catgccgtcg atgactcgct attgcagtac ctgtcgccac tcggctggga gcacatcaac
     6301 ctgaccggtg attacctatg gcgcagcagc gccaagatcg gcgcggggaa gttcaggccg
     6361 ctacggcctc tgcaaccggc ttagcgtgct ttattttccg ttttctgaga cgacccctat
     6421 tatggcgaag gtatcattca ggaagcgttg atgaaggatt atctctgtaa aaagttattc
     6481 aaccggcttt ccggtaccct ggtgatcaga gcgcgctgcg gcaataacat tactggcctg
     6541 gcatgttgca atattcttta tccctcgccc cgatacagcg gtcagctgca tattaaagag
     6601 ctgtatgttt ctcagtgcga tcgaaataag ggcacgggta aagcgataat gcgctttata
     6661 gcccggcttg cgcttgaaca ggaatgcctt agccttagct ggaacgctga aaaatccaac
     6721 cccggcgcta accgttttta tcaggctctg ggaggcagaa taaatgatca tatcgtcaat
     6781 tattacctcc acggggagag cctgagcaaa ctggcctcag gcatttgaga agcacacggt
     6841 cacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta
     6901 acgaccctgc cctgaaccga cgaccgggtc gaatttgctt tcgaatttct gccattcatc
     6961 cgcttattat cacttattca ggcgtagcaa ccaggcgttt aagggcacca ataactgcct
     7021 taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
     7081 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     7141 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     7201 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
     7261 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     7321 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     7381 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     7441 accagctcac cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca
     7501 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     7561 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     7621 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     7681 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     7741 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat
     7801 tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat
     7861 tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgt
//
LOCUS       DAJTSI010000169         7895 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000169 DAJTSI010000000
VERSION     DAJTSI010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7895
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.169"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(150..511)
                     /locus_tag="OO327_003525"
                     /pseudo
     CDS             complement(150..511)
                     /locus_tag="OO327_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(850..1404)
                     /locus_tag="OO327_003526"
                     /pseudo
     CDS             complement(850..1404)
                     /locus_tag="OO327_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1547..1669
                     /locus_tag="OO327_003527"
                     /pseudo
     CDS             1547..1669
                     /locus_tag="OO327_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1931..2497)
                     /gene="ipgB2"
                     /locus_tag="OO327_003528"
     CDS             complement(1931..2497)
                     /gene="ipgB2"
                     /locus_tag="OO327_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCR6019935.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2779..3093)
                     /locus_tag="OO327_003529"
     CDS             complement(2779..3093)
                     /locus_tag="OO327_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019936.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3171..3281)
                     /locus_tag="OO327_003530"
                     /pseudo
     CDS             complement(3171..3281)
                     /locus_tag="OO327_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3605..4282)
                     /gene="ospD1"
                     /locus_tag="OO327_003531"
     CDS             complement(3605..4282)
                     /gene="ospD1"
                     /locus_tag="OO327_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCR6019937.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4654..4791)
                     /locus_tag="OO327_003532"
                     /pseudo
     CDS             complement(4654..4791)
                     /locus_tag="OO327_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(4873..5171)
                     /locus_tag="OO327_003533"
                     /pseudo
     CDS             complement(4873..5171)
                     /locus_tag="OO327_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5760..5919)
                     /locus_tag="OO327_003534"
                     /pseudo
     CDS             complement(5760..5919)
                     /locus_tag="OO327_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5923..7152)
                     /locus_tag="OO327_003535"
                     /pseudo
     CDS             complement(5923..7152)
                     /locus_tag="OO327_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(7172..7519)
                     /gene="tnpB"
                     /locus_tag="OO327_003536"
     CDS             complement(7172..7519)
                     /gene="tnpB"
                     /locus_tag="OO327_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6019938.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLVSKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(7519..>7895)
                     /locus_tag="OO327_003537"
     CDS             complement(7519..>7895)
                     /locus_tag="OO327_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6019939.1"
                     /translation="RRPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQ
                     YRKGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLR
                     LKGKLTPALLQILIREIKGSSH"
BASE COUNT         2025 a         1843 c         1722 g         2305 t
ORIGIN      
        1 ccggctactc attccctgct tgtgcaggat tttaatttcc ataactgtct caaaagtgac
       61 cataagctct cctgaatcag gagagcagat taccccctgg atctgatttc aggcgttggg
      121 tgtggatcac tattgcaccg ttcgtgacag tgttgttgcg ccttgctgcc aacgaagctc
      181 cactgcccat caagttcaca gataagggca acatcagcgt gagcgactgg cgcagatgtt
      241 atccgccggg gcgggagctt tttaatgtcc gtatgacggt attgatgccg attttcagcg
      301 tacggccagt atcgcgaact ccggcaccgt tgaaagccat ttcagtgatt tggtttttaa
      361 ctcccggttt acgggcctcg taacagtaag tgagctgaaa cacccggtga cagtcacggc
      421 aacggaatcg atcatgtcct tttgggttct gcccgtgacg gtaaacctga acggactggc
      481 aacgggggca atgaaagtta actgtgacca tgaaagaacc taaaagcggg tattataacc
      541 cctaattcaa ctaattggag gcattgtcat ttttaatcta tggatacact taaaaatcct
      601 ctcaccttac gttaaaaacc ttctcatgtt tagagttttt tattatgttt atcataatga
      661 tatgacgatc tgagttttta aaatcaagaa gaaaagataa gttttttaaa tgcacagtgc
      721 ctgtggataa ctttgcgtaa atccggagga cagatcactc tgaagcaaga caacagcatc
      781 tgaaaacaat atgctcacgt ttcaaccaca gggcgtaagt gatcctaccc acgtaatatg
      841 gacacaggcc taagcgaggt tctggttttc aaattgttcc ggactgaggc cgccacacca
      901 actgtgccgt cgccagcgat tgtaatcaca ttcgatataa ttaaacactg ttgccctcat
      961 tatttcccgg ctgataaagt gttctccgtg gagacattcc actttcagcg aatgaaagaa
     1021 gctttccacg caggcattat cgtagcagca gccctttgcg ctcatactgc cacgcagatt
     1081 atgccacttc agcagcgcct gataatctgc tgaacagtac tgacttcctc ggtccgagtg
     1141 aacgataacg ttccggggtc tcttacgccg ccacagcgcc atctgcaggg catcgcaggc
     1201 cagttgcgcc gtcaggcgtg gtgacatgga ccagccaata acggcacgtg accacaggtc
     1261 aatgaccaca gccagataca gccagccttc atctgtacgt aagtacgtga tgtctcccgc
     1321 ccacttctgg ttcggaccac tggcgtaaaa atcctgctcc agcagatttt ctgacacagg
     1381 caggccatgt gcgcggtaac tgaccgtatt gctgggtaag atcaatttgc tcactggtat
     1441 ttactacgtg catcgaatga gtgatcaaga gccgtatgac gcgagagtgt cacgtacggt
     1501 tccgtaagca gccggagagt gaaactctct ccggctgact cgactcgcag gttccgacag
     1561 cggtgacgaa catgcagcgg tgttgttctc gctgatcggc acatgccgtc tgaacaatgt
     1621 ggagccagaa aaatggctgc gttacgttat tgaacatatc caggactgac cggcaaaccg
     1681 ggtacgcgat ctgttgcctt ggaaagttga tctgacttct cagtaaatat caatatgatt
     1741 ctgacgagcc gcttaccttt gagcagagaa aggggagagg gatcccacat ccatgagcta
     1801 ggaaaataca aaaactggct atacaacaat tcaatatatt aggacttact tgcgaggaga
     1861 aatatagcac cgctcaggca attcctgggt atttactttt cttagtttct ttactcggga
     1921 tgttctgtca tcagaaaggc gattctaaat ttgtaatata gtcactcata ttcccacgta
     1981 atttctttag atcaatatat tgcacatttt tctcaaaaaa ctgatcggaa ataagatttg
     2041 caacattgct tgttgtatca tttttaacgc ttctattcat cccttcacaa agttcgtcca
     2101 ttttcttttt gaaataactg cttcctgata caagatgatt tatggaactt tgtgcagcat
     2161 tgatgtctag agaaatgcga taatctttct caacctgttt aaatatctgt ttacggagat
     2221 tatcgccgat ttttcggctg tgttctgcac aaaacttatc aatacattgg tttaccaccc
     2281 gggatacgta gacttttttt tcatcatttt tccaatctgt aatattttca ccgatttgtt
     2341 ctgaaacaga gctcaaaata ctagtattta ttttggttga tgatattttt actatccttt
     2401 tccccattgt acagcggata atctcatcca cctttcctga aaaatatctt ttgtgagata
     2461 aactgattcc aaaattatta aaagatgttc caagcattat aatcaccttg tcatgtaaaa
     2521 tagtaacatt gtcattattt tagctgattc aaacaattct gtaaatgctt tgagttcttt
     2581 actcccttgg ctcaatgatg gaccgtctgg aatcccagag actaaactct ctgagaaaga
     2641 gagtatgaaa aaacttgggg cagtccctaa atgaagggac caacagcggt aaaataaccg
     2701 gcatcattca taatgatgcc ctgttagttg tagttatgaa gatttacgtc attaaaattg
     2761 ctgttcgtag tgtcagtcct atggtctggc atcagctgag gatcgtagcc gatacatcac
     2821 tggcttcacg acattcgtgt tgaggctatt catgaaaact ccacgctgaa aacgccgttt
     2881 tgtataaacg gtcacggtat gcccggagcc actgttgctg ataaattcga taaaaccttg
     2941 gcgtttcttg aagctatcgt taatgcggat aatgaaacaa cgattggtga gattcgctct
     3001 tttgccgacg ccctggacgc tgtccggttc aatcgcaaca aaatcaaccg gcagctgagc
     3061 aaacccaacc ttgcctctct ggcgctggag catgaagtga tttggctggg acgtagccgc
     3121 tgacagcttc tataaaagta tgatcctacc cacgtaatat gaacacaggc ttaagtgagg
     3181 ttctggtttt caaattgttc aggactgaga cctccacacc aactgtgccg tcgtcagcga
     3241 ttgtaatcgc acttgatata attaaacact gtatatttca tttgtggagt ctccagtctg
     3301 agagggcttt atgcattccg tattgggcac tatgatgcca gaaatctgca aggctccaca
     3361 ccttcctgta tttcccttcc tctatcacag tgcactttac ttaatagggt gggatgtgtt
     3421 cattacattt attgtgtttt ttatgattgt ttttgcgtcc agtgtcgttc gtcatgggaa
     3481 actggttcta tgggtagtat cgctcagtct agttaactgt ttgtttcttt ttagaaatta
     3541 agcagatcac acaattcggg gagagatcct tctaagtgca attctttgca gctatttagc
     3601 acacttaaaa caactcttgt attttgtcag aaataatatt aaacatgtct tgttcattat
     3661 tgttaagtct gtctgctatt tctgtaagtt ttatcccact atgatggcat atatttatat
     3721 atgccatcat agcctcgata ttccctttcc ctgcggccag aaataatcct ggtaacccct
     3781 cattatctct tacatccaat agtttaatcg tttcttttgg tgataaatca aaagttttaa
     3841 taaaatctcc ataagcttga atcgcagcgg aatgaccttc ttgaagagct atgaacaaag
     3901 cagattcccc agcattattt cgtgctgcaa gaacttctag tttttcctct ttgggtattt
     3961 cggctgccaa cagcgattca ccgtatttac gcgctgcatc ggcatttcca tgccaaatca
     4021 atgaaaacaa attatggcgg ttatactcct tattattctt tgtcggaatg gatatgttct
     4081 gtattttggc caattttctg aaagaaacac cactatatcc cccccctgat ttttcttcat
     4141 tgatggcgtg ggatatagcg gtatttgtca cgttaatgat attatttttc attcctttat
     4201 tttcattagc agtattgctg cctattatta agtgcatatt tttgttgttt gctggatgta
     4261 atccatagtt atttattgac atttttgttc atcctttttg ctaaaaaaaa acaataacct
     4321 atttttgtaa tgtaatagat attagtctat tgctaatcta aaaaaaccat tgccacattg
     4381 aatggctata ttcaccaata aattctttcg atttcgtata ttgacatatc gtagatatat
     4441 cttatcccta agattcaata atcgagtccg tttaccattc tgtgcaagcg ccttcactca
     4501 gaagtgaccg tcaaggcgat caccaaactt gataataaag cagctcattg ccatacgcca
     4561 gtccttaagg ggcgttgtcc atttctgtga tgctgcctag atttaactgc cagctccggc
     4621 ttatctacat ctaaatatgt gagcctaata ccgctacact ttgtccagac tatgtttttc
     4681 tccgggaagt gggctaaccg tctccataaa gtgacgaact tttttcaaca gtggccatat
     4741 tgaacggcac tgatgattac gggttattgt ctcatgaaga gccggccaca tcactgacag
     4801 cagtgacttc aggccttcag caccagacag cctgaaccag ttaatccatc gcccgacaaa
     4861 tttagtaatc tagggctggt ttttcttcag ctgttgcggt tcgaaagtgc cattacgatc
     4921 acgcagagta cggatttcca gaggttcatc tccagtagtg ataatcttcg ttaagcaacc
     4981 attgcgggaa ttgctgccag atctaagctg atttttctcg cacccaagat gatgagtcat
     5041 attagcattg agtacggttt cattttcagc atccaatcaa actgactgag atcttcagga
     5101 tttttgagac ttttggctag ttcattagct aaagcccgca acttattaga tgtcaactgt
     5161 ttttcattca taaattcacc ttcatttgat atcaaattaa atagttcaac atcagagaat
     5221 tatacaaact tatgtacagg ctcctatgat cttcgaatcc tgaagattat agtgaaaata
     5281 agttttcacg ttgaaatccc acatagttag tatatgataa ataatcatgg atgacctgat
     5341 taacctcttg gggtagtgct tttatagaag aataaatatt ctactaattt aatacatcac
     5401 ctaaaatagt attgtttttt atggttggtt gaaggtcgtg gtgatgatgt tagtcctttt
     5461 gagaatattc cagtgccaga tcttacttat aataaagggg cttggcagtt tggataaact
     5521 attgttctta ttccactaat acaaactttt tgtaaagtat attaaatata cttcatttca
     5581 tactgaaatc cccggaagta ttcaatcaaa caattacacc ataaaaggaa gagggggccc
     5641 taattaagtt gtcaagcatg tttatgatac ctgcagggta taaattttct ggctgcaagc
     5701 agacacatat aaaacgtctt tattgatcct acccacataa tatggacaca gtcgtaagtg
     5761 aggctctggt tttcaaattg ttccggactg agaccaccac accaacggtg ccgtcgccag
     5821 cgattgtaat cgcactcgat ataactaaac actgttgccc gcactatttc ccgatggata
     5881 atagtgttct ccgtggagac attccatttt cagcgaatga attttcatct gttgctgtat
     5941 ccagtcatac agtgactgca tcagtggcgc ggctctggct tttcttgccg ccagacgctg
     6001 ttctgctgaa catccccgga cttctgcctc gatggcatac agttcaccga tacgctgcag
     6061 ggcttccgtg gtgatgtcgg ttggcactct tgcatgcaca tcgtggattt ttctccggac
     6121 atgagccata catgcggctt ccgttattct gccggattcg tacaacgccc ggtaaccacc
     6181 gtaagcatcg gcctgaagca ctccgctgta accggccaga tgattctgtg gatggatacc
     6241 tttccggtcc ggtgagtacg cgaaccagac cgccggaggc atttccgaac cggcgttacg
     6301 gtcatcacga acgtagaccc acagccgggc agtccttgtt ttaccgctgc ccggctcctg
     6361 gaccggaacg gggatatcat cggcatggac tttaccgggc atcagcacat actggcgcag
     6421 gatgtcatac agcggttcca gcagttcagc aacagcacct gtccagcgcc ccagtgtggc
     6481 acggctcagc tccacgccct gacgacggta tatttctgac tggcggtata acggcagata
     6541 gtctgcatat tttccagcaa caacatgggc cagaagcccc gctccggcat aactgcgtgc
     6601 aatgggtttt gaaggcaccg gtgcctgcac gatatggtca caccggcaac aggccagttt
     6661 cggacgttgt gtttcgataa ccttaaaggc gctgctgata agctccagct gctctgacac
     6721 atcacatccc agagaactga gttcgccacc acaggccggg cagcattcct cttccggacg
     6781 gatgacccgg gtttcacggg gaagtgaggc tggtaacggt ttacgggctg aagactggcg
     6841 caggggagat ggcagtaccg ggtcatattg ctcacccagc gtttccgcca tttcttcctg
     6901 aagtgcgctg attcgctcct gtgcttccag tatctgccgt tcggtttttg cgcgaagttt
     6961 ttctgagctt ttaccgaact gcatgcgctg cagtttcgca accagcgcct tcagccggtt
     7021 gatttcggaa gcataagccg ccacccgctg tgagagcagg cggttgtatt tcgccatctg
     7081 gcggatggtg tcctgttgcg tctgcaacag tgcccgcagg cgggcattct catgagcaag
     7141 tgaggtgtcc atatcctcac tttacaacgg gttatatgcg gatgccagcg cgttccgttc
     7201 gtttcgggtg tttccagtcg atgccctcaa gaagcatgga taactgggcc ggagtaaggt
     7261 gcaccttgcc gtcacgggtg accggccaga cgaagcggcc ccgctccagg cgtctggtaa
     7321 agaggcacag tccgtcactg tcagcccaca acacttttat ctggtcaccc cggcgtccgc
     7381 ggaagatgaa cagatgcccg gagaacgggt catccttcag gacgttctga acttttgaga
     7441 ccaggccgtt aaagccattt cgcatatcgg tgatacctgc aaccagccag atacgcgaac
     7501 ctgcagggaa agatatcatc agtggctgct cccttttatt tcgcggataa gtatctgtaa
     7561 taacgccggc gtcagtttac ctttaagcct gagagttccg gccggcagaa ccagctcaca
     7621 gcacagactg tcggacggtg tatttatctg ctctggttcc tgtgccggag ccgggatttt
     7681 attatccggc tccggcgtta acgtcacggg aagcagtgca ggcatatttt ttccggaagg
     7741 cagcaggcca cctttccggt attgatggcg ccagttgaag agcaggttat cgttgattcc
     7801 gttttcacgg gcgatctgcg ccacacaggc tccgggctgc agtgactgct ccactaaagc
     7861 gattttaaat tcataaggaa aattaggccg ccggt
//
LOCUS       DAJTSI010000170         7781 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000170 DAJTSI010000000
VERSION     DAJTSI010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7781
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.170"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1331
                     /gene="yjeM"
                     /locus_tag="OO327_003538"
                     /pseudo
     CDS             1..1331
                     /gene="yjeM"
                     /locus_tag="OO327_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="OO327_003539"
     CDS             complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="OO327_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR6019940.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNRQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4815..5783)
                     /gene="psd"
                     /locus_tag="OO327_003540"
     CDS             complement(4815..5783)
                     /gene="psd"
                     /locus_tag="OO327_003540"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR6019941.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="OO327_003541"
     CDS             complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="OO327_003541"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR6019942.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7027..7572
                     /gene="orn"
                     /locus_tag="OO327_003542"
     CDS             7027..7572
                     /gene="orn"
                     /locus_tag="OO327_003542"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR6019943.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         1762 a         2017 c         2104 g         1898 t
ORIGIN      
        1 ttaatgatgg ctgaaatggg agctgcttat cgcaaagaag aaggtggtat ctattcctgg
       61 atgaataata gtgtcggacc acgttttgcc ttcattggta cgtttatgtg gttttcctct
      121 tatatcatct ggatggtgag tacctccgcg aaagtttggg taccgttctc aacattcctc
      181 tatggtagtg acatgaccca gcactggcgt attgccggac tggagcctac gcaggtggtt
      241 ggtctgctgg cagtagcatg gatgattctg gtcacagtcg ttgcttcaaa ggggattaat
      301 aaaattgccc gcattactgc ggtgggcggt attgcagtaa tgtgtctgaa tttagtattg
      361 ctgttagtaa gcattactat tttgttatta aatggtgggc atttcgcgca ggatattaat
      421 ttccttgcat caccgaaccc gggttatcag tccggtctgg caatgctatc gtttgtggta
      481 tttgctattt ttgcctatgg cggaattgaa gcggttggtg gtctggtcga taaaacggaa
      541 aatccagaaa agaactttgc caaaggtatt gtttttgccg ctattgttat ttcaatcggt
      601 tattcgctgg caatattttt atggggcgtc agcacaaact ggcagcaggt attaagtaat
      661 ggttccgtta accttggcaa tattacctat gtgctgatga agagcctcgg ggtgatgctg
      721 ggtaacgcac tgcatttgtc acctgaagcg tcattgtcgc tgggcgtatg gtttgcgcgt
      781 attaccggac tttcgatgtt cctcgcttat accggtgcgt ttgctattca ccgctgaaag
      841 ccatcatcca ggggacgccg aaagcgttgt ggccggaacc gatgacgcgc ctgaatgcga
      901 tggggatgcc gtctatcgcc atgtggatgc agtgcgggtt ggttactgtc ttcattctgc
      961 tggtttcgtt tggtggcggt accgcatcgg cgttctttaa caagctgacg ctgatggcga
     1021 acgtgtctat gacgcttcct tacctgttcc tcgcgctggc tttcccgttt tttaaagcac
     1081 gtcaggatct cgacagacca tttgtgattt tcaaaacgcg tatgtcggca atgattgcga
     1141 cggtggttgt cgttctggtg gtgacatttg cgaacgtctt caccattatt cagcctgtgg
     1201 ttgaagccgg agactgggac agcacattgt ggatgattgg cggccctgtc ttcttctcgc
     1261 tgttagcgat ggcgatttac cagaactatt gcagtcgcat ggcgaataaa cctgagttag
     1321 ctctcgactg atcatatgaa atcctgcaac tcatcgtctc tccggcgatg agttgtcagg
     1381 aagtaatgcg tatggatgat actctccagg gccggtgata actgaagatg atgttaagct
     1441 gctaaatgag ccacgttgac atcatttggc agcttcatgc attacaaact tcccgcctga
     1501 cgacctttgc ccgcagacgt cagcgttctc cccgtttgtt taccgttgag gctttccaga
     1561 cgcatctgga agggcgggaa tggcatatcg ataccgtggg cgtggaagcc tgccagaatc
     1621 agctggtgga tctcatggcg tagcggcata cggtgaccca tctcagcggc gtaaatacgc
     1681 agctcgaaaa tctgaatccc ctgttgcaga tccaccagga agacttccgg tgccgggttg
     1741 tcgatcacca gcgagcagcg acgcgctgcg gtgagcagga tttccgtcac ttcttcactg
     1801 ttggcatcgg caggggccgg aatcgtcaac actacgcgcg tgaccgagtc agagagcgac
     1861 cagttgataa actgctcggt aataaacgcc ttgttcggca cgattatctc tttacggtcc
     1921 cagtcgctga tggtggtggc gcgggtgtta atttttgtca cgctaccagt aagatcgcgg
     1981 atcgttaccg tatcgccaat gcgaatcggt ttttcgaaca ggatgatcag gccagagata
     2041 aagttggcga aaatttcctg caaaccaaaa ccgagaccaa caccgagcgc ggcaaccagc
     2101 cactgcaatt tcgaccactc aataccaatc attgagaagc cgaccagccc gccaatcagc
     2161 atcagcagat attttgtgat ggtggtgatg gcgtaacccg tacccggcgt taaatccagg
     2221 tgctgcaaaa tcgccagttc cagcagcgcg ggcaggttgc gcaccagctg cgtggtgatg
     2281 ataaatacca gaatggcaat cagcaccgca ccgagggtaa ttggctccag actttctacg
     2341 ccctgtaccg tggaggtgac atcccacagc gaaatatttt cgaggaagcc gaaagcggaa
     2401 tggatttctg accacagcac aatgaccgaa agcagggcga tcaacatcaa aattgagcgc
     2461 accagccgca aggattgcgc actgatggca tcgagatcga cttcgctttc atcgacttca
     2521 attgctcctt ccgggctact gtgatgatgc gcttcctctt cgccacgcgc acgttgcgct
     2581 aacatctctg cccggcgatg cttcgcccgg tcaaacgcca gcctgcgacg ctggatcagc
     2641 atccagcggc ggataacgtg ataaaccacc agtagcagga accagatggc aaccgaggtt
     2701 tcaagcctcg ctaacagtgc ctgcgccgtt gccagataac ccaccgccga cgccagaatg
     2761 gcaaccagtg gtgcgccaat catcatgttc cacagcatat ggttggtaat gttgtcgccg
     2821 ctgccttctt tgttgagata caacggaata ccggcttttt tcaggcttag ggtgaccacc
     2881 gccagcgcac cgcaaatgag gataaagcaa agccgtccca gcgaaccgga aaattcgcgg
     2941 tcgtcgaggt tatcgaacat catcagcgcc ataatcagcc cgatgctcat caggtagtag
     3001 cgcatcccac gggaaacacg ttcgcgcggc cagccaaaat gagcgataaa caagccgttc
     3061 gggcgggcaa aggtggcgca aatcatcact acccacagca gcggcacggt ggccgttaca
     3121 ccatcgccaa tcgcgaccgc cagcggataa ggccacgcct cgcgcaagcc gtaacccagc
     3181 gtcatccaca gcaccggtaa cggtgacgcg acgagaatcg accagaaaag agtgcgcaac
     3241 gtcagccaga agtgatcctg agtcactttg ccgactttcg ccgccgaacg ttcaagaaaa
     3301 cgggtgaaat agcggcgcga gtaaatactg caaccgacca gaatcaacgc gccaaacagc
     3361 ggcaaaattg tctctttgct ggtcagcatc atcacactgg ctttgcccaa ctgactgaag
     3421 gtgtccagcg aaatgagacg acgcagatcc tgggcgattt ccagcggcca ggcgatggtc
     3481 atcgggcgca cgtcagaggc ccagaacaga tagcggtgcg ttgcttcgtt cacctctttc
     3541 agcgcatcct ccagttgcct gttggagact ttcagcttgg tcagttccaa tagtagcgtg
     3601 tcgccaccct gcaacaatga gttcagcaac tcacgctgag tgcgcagttg tgcttccaga
     3661 atacggtttt gctcggcggt cagcggctga ccgtcggcct gatggatttg ccgtagcagc
     3721 ggctgtttat taagtaaatc ctcataacgt aaccgttgca cacgcaactg cgccatttcg
     3781 gtatcaagct gttgtggttt tggcatttct ggtagccgtg ccacctgcgc ccgcagcgct
     3841 tcgccgagca ggttggacga tcccagccat tgcgactgtt cacgcagcgt attcaacgcc
     3901 tgccggacct gtaacgtctg gctggcagcc tgacgctgtt gcgaggcaac gagctccatc
     3961 cgctgcgcct gttgattcag cgctgccgat agttcgcggt taattttgaa ttgcgcgacg
     4021 atatctttcg gcaaatcggc actgttttct gccagtaatt cggtactttc cagcgcccgc
     4081 tccgcctcaa gctgacgttg gctattcaac tgattacgta aggcctgcaa atacgcatcc
     4141 agttgctggc tctctttttc cgccagctct gagcgtaagc gcgctaattc ctggcggtta
     4201 ttggcagaca gctgcgccag ctccagttca tcaacgagcg ccttaagacg tgcagagtca
     4261 gactgcaacg cgaaattttg tgcctgattg agcggagtat tgccggtaag cgttcccagg
     4321 cggcgctcga tctcatttaa ctgacggcgg gcgtcggttt gctgttgcgg cagttgattc
     4381 agcgaatcgg caatctcgcg ggcgcgctcc tgctcttgct gggcctgacg gcttttatcc
     4441 agcaactggc tgctgacctg gagaatttcc tgattcagcg cgtcggtaga cattcccggc
     4501 gacacgctgc gcggctcgtc acgaatgttg tttaattgtg cgcgcagagt agcggagagt
     4561 ttcggataat tatcgataac ttgctgatat tgtttgatgc gctcaaggga accttttcgt
     4621 tcctcaagcg catttaaggc agactggagc gcctctacga cttccggctg tgcgggtttc
     4681 gccgcttttg cctgctccag ttcctgagtg atttgtttgc tatcgggggc cgtcgcggcg
     4741 tacgcccccc aactgaggca ccaggccatc agaaaagtga taatcaggcg cacgtcagcg
     4801 tttcctttga tggattagac ctggtctttt ttatcgtcaa ccaatgggct ggcgtcgtgt
     4861 tctgcttcga tctcttcagc aggaagtggg gcgggttcag cgtctggcgt aacaaaggtt
     4921 tcggtagata ctgccagcgg ctggccaatt ttcgtgacag acaggctttc cagttgctca
     4981 accagattca ctttacccgg tgcaaacagg ttgataacgg tggaaccgag tttaaagcga
     5041 cccatttcct ggcctttcag cagtgccaca gaaccgtcgt tttccccggc aggccaggtc
     5101 cagcgcttga tgataccttc gcgcggcggc gtaatggtgc ccgcccagac cgtctcaatg
     5161 ctgccaacaa tcgtcgctcc gaccagaatc tgcgccattg ggccaaattc ggtatcgaaa
     5221 aggcaaatca cgcgttcgtt acgggcaaac agatttggca cgttctgagc ggtgagatgg
     5281 ttaacggaaa agagatcgcc cggcacgtag atcatctcac gcagaatacc gttgcacggc
     5341 atgtgtacgc gatggtagtc acgcggggag aggtaagtgg tcacaaacgt accgttgcgg
     5401 aacaggtccg ccatcagata gttgcctgcc agcagggctt cgaggctgta gttgtggcct
     5461 ttggcttgca ggattttatc ttcttcgatt ttacccaact ggctgataac gccatcggca
     5521 ggcatgacca gtacattcgg atcggtatcg attgggcgta cttcgtcacg cagcggacgg
     5581 acaaagaatt cgttaaaggt gcggtagctg gcggtgtccg gcttttgcgc ctctttcatg
     5641 tcgaccttgt agtatttaac gaacagatcg ataaccagtt ttgtcagcca tcctgcccgc
     5701 ttgcttgcgc cccaacccgc caggcgagta agccatagtt tcggcagaat gtactgtagc
     5761 gaaagtttaa atgaatttaa caaggtagcc tccaggccat tgttttgtcg ttcctgatcc
     5821 ggccaacatg ccggatcctg aaaaaaaggg gacgattcta acgacggtta gcttaattgt
     5881 cagtcatccg tatcagaaaa gtttttacgc gtttttacct gcgccatgct ttccagaata
     5941 cggtgatagt tttcgaaacg ggtttccgcg attttccctt cctcaaccgc ttcccggata
     6001 gcgcagcccg gatcggtatc gtgtttgcaa tcgcgatatt tacacagacc taaatagtca
     6061 tggaattcga caaagccctg agtgatttgt tccggctcca ggtgccagag gccgaactca
     6121 cgcacaccag gggaatcaat cacatcaccg ccgtgcggga agtgatacag ccgagcggcg
     6181 gtcgtggtgt gctgaccgag ccccgagttg tcagagacat cgttggtcag gatctctttt
     6241 tgcagcccca gcaacgcgtt cagcaggctg gatttaccga cgccagactg cccggcaaaa
     6301 atgctgatgc gcccggtcaa cgcctcttcc agcggtttca gcccatcctg agtatggctg
     6361 gaaaccatca atacgcgata accgatgttg cggtagatat ccatctgctc gttgacgaac
     6421 gccatgcctt cgtcgtccag caggtctatc ttgttgagca cgataatcgg ctcaatctgc
     6481 aaggtttcgc aggccaccag gtaacggtcg ataatattga gcgacagctc cggcaaaatg
     6541 gcggagacaa tgacaatctg gtcgatgttg gcggctatgg gtttcacgcc gtcgtagaaa
     6601 tccggacgcg ttaacaccga ggtgcgctca tgcaccgctt ccacgatccc tttgacattt
     6661 acgccttccg ccgccggttt acccggacgc cagactacgc ggtcgccggt taccagcgaa
     6721 cggatggtac ggcgaatatt gcagcggtga acgtcgccat cggcggattc cacatcagcg
     6781 tgcataccaa agcggctgat gacgatacct tcatcaggct cgccaaacag attgtcgtcg
     6841 tagtcgggct tctccttaga cgttttaaga cgacgctggt gattggcgtt cacgcggcgc
     6901 tgctggcctt tggagagttt atttttactc aatcgtactg actcctggtc gcccctgatg
     6961 ggcaaaacat ctatgataca cgcaattgtg gatcaatata gtcactgtga atgggtggaa
     7021 aatagcatga gtgccaatga aaacaacctg atttggatcg atcttgagat gaccggtctg
     7081 gatcccgagc gcgatcgcat tattgagatt gccacgctgg tgaccgatgc caatctgaat
     7141 attctggcag aagggccgac cattgcagta catcagtccg atgaacagct ggcgctgatg
     7201 gatgactgga acgtgcgcac ccataccgcc agcgggctgg tagagcgcgt gaaagcgagc
     7261 acgatgggcg atcgggaagc tgaactggca acgctcgaat tcttaaaaca gtgggtgcct
     7321 gcgggaaaat cgccgatttg cggtaacagc atcggacagg accgtcgttt cctgtttaaa
     7381 tacatgccgg agctggaagt ctacttccac taccgttatc tcgatgtcag caccctgaaa
     7441 gagctggcgc gccgctggaa gccggaaatt ctggatggtt ttaccaagca ggggacgcat
     7501 caggcgatgg atgatatccg tgaatcggtg gcggagctgg cttactaccg cgagcatttt
     7561 atcaagctgt aaaatttttc ctggtcacgt aagcgccgga atccacttgc ggcggcgtga
     7621 ttttgacgct aaatcgaacg tcttgctgat aatttcagca cttgagataa aaacgcaaaa
     7681 aaaacttttt tggggggttg cagagggaaa gatttctcgt ataatgcgcc tcccgtaacg
     7741 acgcagaaat gcgaaaatta cgaaagcaaa attaagtagt a
//
LOCUS       DAJTSI010000171         7764 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000171 DAJTSI010000000
VERSION     DAJTSI010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7764
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.171"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..282)
                     /gene="gltI"
                     /locus_tag="OO327_003543"
     CDS             complement(<1..282)
                     /gene="gltI"
                     /locus_tag="OO327_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR6019944.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPIT"
     gene            complement(679..2217)
                     /gene="lnt"
                     /locus_tag="OO327_003544"
     CDS             complement(679..2217)
                     /gene="lnt"
                     /locus_tag="OO327_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR6019945.1"
                     /translation="MDFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            complement(2242..3120)
                     /gene="corC"
                     /locus_tag="OO327_003545"
     CDS             complement(2242..3120)
                     /gene="corC"
                     /locus_tag="OO327_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR6019946.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(3210..3677)
                     /gene="ybeY"
                     /locus_tag="OO327_003546"
     CDS             complement(3210..3677)
                     /gene="ybeY"
                     /locus_tag="OO327_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR6019947.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(3674..4714)
                     /locus_tag="OO327_003547"
     CDS             complement(3674..4714)
                     /locus_tag="OO327_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR6019948.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            complement(4867..6291)
                     /gene="miaB"
                     /locus_tag="OO327_003548"
     CDS             complement(4867..6291)
                     /gene="miaB"
                     /locus_tag="OO327_003548"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR6019949.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            6437..7612
                     /gene="ubiF"
                     /locus_tag="OO327_003549"
     CDS             6437..7612
                     /gene="ubiF"
                     /locus_tag="OO327_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR6019950.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
BASE COUNT         1771 a         2037 c         2090 g         1866 t
ORIGIN      
        1 ggtaatcgga atcagtttta cctgcaagtc cggtttgttg agtttctttt tcactgcttc
       61 aacaatggcg ttggagtaat cctgcgagta acccaccact ttttgctgat tgtcgtaata
      121 agagaaaggt actgaagatt cacggtgacc gacgacaatc acaccgtttt tggcgatttt
      181 gtccagcgta ctgcctgctg ccggggcggc gtcatctgcc tgtgccagtc ctgcggaaag
      241 cgccagggcg aggattgctg tggcaggttt acgtaattgc atatccaaca cctttatctt
      301 ctgcgttaag aacgcatgga tacccgttgt gagtgtttgt gttgttatcg tctgcaactt
      361 tattgtgcag tgttgtgcct gttaggtaac atttagtttg gctaaatgta aagatattga
      421 tgttttattg tttgtttttg cgaggtgcac cgcaccattc tgaagcaaaa ttcttaaaat
      481 gcactctttt ggtgctaccg gtggattgct gtggtgcaac cgggttgtac tgatgctggt
      541 tcaagctttg ccttgtataa caaagcaata gatgtgccaa agttggatag gagaatattg
      601 ttatccggat aatgcactga tgccgcatcc ggtgagcgtg ccgaaatatg ggatgtattc
      661 cggcacgata agaagggatt atttacgtcg ctgacgcaga ctcatcaaca cagcagcaaa
      721 accaaacagt gccgtcagca cccacagcgg ccagttgccg gtacgtgcgt atggtgtgag
      781 tccggtggtc ggcgtcacgt tagtggttaa cacctcgcgg gcgaactgcg ggatcatcgc
      841 ctgaatctca ccctgcgggc caatcaccgc cgtaatgccg ttgttggtgc tgcgcaacag
      901 tgggcgcgcc agctccagcg cacgcattcg cgccatctgg aagtgttgcc atggaccaat
      961 agatttacca aaccacgcat cgttggagat agtcagcagg tagtcggtat ccgggcggaa
     1021 gttatcgcgc acttgctcgg cgagaataat ctcgtagcaa atagccgcag taagctcaat
     1081 accatttgcc gacagcggcg gctggatata tggcccacgg ctgaacgacg acatcggcag
     1141 atcaaagaac ggtgctaacg gacgcagaat cgactccagc gggacaaact cgccaaacgg
     1201 caccagatgg tttttgttat agcgatcggc tgattcgtag ctgtacggtg cacctttacc
     1261 cagcgtgatg atggtgttgt aggtatcgta gcggttctgc ttattgagac gcgcgtcgac
     1321 aatcccggtt accagcgagc tacctttatc acgcaactca ccgtccagtg ctttgaggaa
     1381 cggttgctga ttaatttcca gatcggttat cgccgactcc ggccagataa tcaacgatga
     1441 tttgcccatc agcggtgccg ttgcgttgta gtaaatcttt agcgtattaa gaagctggcc
     1501 ttcgtcccat ttcagcgatt gcggaatatc gccctgaacc atcgaaacct gaatggtttt
     1561 ctccggttgc ggggtaaacc attggatgta acgcagcggg aagggtaggg caaacagcac
     1621 gacggccacc accagcggac gccagttgcg tttgaccaac gccagtgcca gcaggccact
     1681 aaccatcatc agcaggaagt taatggcttc cacgcccatt agcggtgcca gcccttttaa
     1741 cgggccatca atctggctat agccgaactg taaccacggg aagccagtca gcacccaacc
     1801 gcgcagaaac tcggtcactt gccagagagc aggggtggca atcgccacgc gcagccaggt
     1861 ggttttcggc cacagacgcg acagcacgcc agcaaacagt ccggtataca gcgacaaata
     1921 cgccgccagc agcaccacca ggaagatgtt aaccgggcca ggcatcccgc caaaggtcgc
     1981 gatgctgaca tagacccagt taataccgct gccaaagagg ccaaatcccc agcaaaagcc
     2041 aatagcggca gactggagtg ggcgacggtt aaaggtcaac gcctgaagcc ccatcagcga
     2101 aataatcgcc gcaggccaga cgtcgtaagg agagaaggcc agcgttccgc aggcaccgaa
     2161 taataacgcc agcagcaggc gaatgcgctg gcgttcaatt aatgaggcaa aatccatgta
     2221 gttatctatc cagtttcggt tttattcatc cagctttggc tggggtgagt catccgggat
     2281 tttgacatga acctgaataa tacgccgact atcggccatc gccactttga actggtaacc
     2341 gtcgatgtcg atagtttcgc cacgcgccgg aagatgccca aacgcctgca tcaccagacc
     2401 accgatagtg tcgacttctt catcgctaaa gtgggtgccg aacgcttcgt tgaagtcttc
     2461 aatggaagcc agtgcgcgca cggtccaggt atgacgactc agctgacgga agtcgatatc
     2521 atcttcttcg tcatactcgt cttcaatctc accaacaatc agttccagga tgtcttcaat
     2581 ggtcaccaga ccggaaaccc caccgaattc gtcaataacg atcgccatgt ggtaacgctg
     2641 agagcgaaac tctttcagca tccggtctac gcgcttactt tcaggaacga caaccgcctg
     2701 acgtaacact ttgtccatgc tgaaggcttc agcatcgctg cgcataaacg gcagcaagtc
     2761 tttcgccatc agaatccctt caatgtgatc tttgtcttcg ctaatcaccg ggaaacgtga
     2821 gtgggcggac tcgatgatga catcaaggca ttcgtccagc gtctggttgc gtttcagggt
     2881 aatcatctgg gagcggggga tcatgatgtc gcgaacgcgt tggtctgcga tgtccatcac
     2941 cccttcgagc atatcgcgcg tatcttcgtc gataaggtcg ttctgcccgg aatcacggat
     3001 cagcgccagc agttcgtcac ggtttttcgg ttcaccgtgg aaaagttggc tgagtaacag
     3061 ggagaaaaat cccttcttgt tgcttatcgt gtcactactg tgtgaattgt cgtcgctcat
     3121 ggcgtcgtat gggttctcat gttagttaat caaaacgccg tcgttaatca ccaacggcgg
     3181 ggacgtctgc cagtcaaatg cctggcaaat tattctttct cggcaatgta cggatcctca
     3241 tagcccagag caagcataat ctctgtttcg agggcttcca tttcttctgc ttcgtcatct
     3301 tcaatgtgat cgtaacctaa caaatgcaga ctgccgtgca ccaccatatg cgcccagtgc
     3361 gcctccagtg gtttgccttg ctcctgagct tccttctcaa ccacctgacg gcagataacc
     3421 agatcgccca gtagcgacat ctccatgcca ggcggcactt caaacgggaa ggagagcacg
     3481 ttggtcggct tatccttacc gcgataggtc agattcagac tgtggctttc ggcggtatcg
     3541 accacgcgaa tagtcacttc cgactcttcc tgaaactgcg ggatcaccgc attcagccat
     3601 gtttgaaact ggctctcttc cggtaacccg gaattatctt cacatgccag ttgtaaatcg
     3661 aggatcacct gactcatttt tgttcctgtt cttcgcgctt gcgttctgct gccagcgccg
     3721 ctttacgttt ttgttctgct tcttcccagg cttcataagc gttaacgata cgcgccacca
     3781 ccgggtgacg aaccacgtct tcgctgtgga agaagttaaa gctgatctct tcgacatcgg
     3841 ccagcacttc aatggcatga cgtaagcctg atttagtatt acgcggcagg tcgatctgtg
     3901 tgacgtcgcc ggtgataacc gcttttgagt taaaaccgat acgggtcagg aacatcttca
     3961 tctgttcgat ggtagtgttc tggctctcat cgagaatgat aaacgcgtcg ttcagcgtac
     4021 gaccacgcat ataggccagc ggcgcgactt caataacgtt gcgctcaatc agtttctcga
     4081 ctttctcaaa gcccagcatt tcaaacagcg cgtcgtacag tgggcgcaga tacgggtcta
     4141 ctttctggct taaatcgcca ggcaggaagc ccagtttctc accggcttcg accgccggac
     4201 gagtcagcag aatacggcga atttcctgac gctccagggc atcaactgcc gcagccactg
     4261 ccaggtaggt tttacccgta cccgccgggc caacgccgaa ggtaatgtca tggtcgagaa
     4321 tattggcgat gtactgcgcc tggtttggcg tgcgcggctt aattacgccg cgtttagttt
     4381 tgatattgac cgctttgccg tactccggca cgctctccgc gctttgctcc agtacccgcg
     4441 cttctttaat cgcaaggtgg atctgttccg gttcgatatc ctgaatctga ccgcgcatcg
     4501 gggcagtatc gacatacagg ctacgcagaa tgtctgccgc agcggtgacg caaatcggac
     4561 ggccggtcag tttaaagtgg ttatcgcggc gattgatctc gatgccgaga cggcgttcga
     4621 gctgcttgat gttgtcatca aacgggccgc acaggctcaa cagacgcgca ttgtctgctg
     4681 gctccagggt gatttcgcga gtgtctatgt tcaaaccgtt cctcttatct gtatgccgcc
     4741 ggaagctgaa cattcaccgg cctataagga aattattcac gccacaggaa aaaggcgcaa
     4801 gcattgcgat aaagatgggg ataaagagag aaaaaacaag gcccaccgga acggcaggcc
     4861 tgagaattac ggctgataat aacccacgcc aaggtcgttt tctttgcggg tgcgggcaat
     4921 caccgactcc ggtgtttctg ccatgcgcag acccatttca tcttcagttc gcaccacttt
     4981 accgcgcaga gagttcgggt agacgtcggt aatttctaca tcgacgaatt taccgatcat
     5041 atccggtgtg ccctcgaagt tgaccacgcg gttattttcg gtacgcccgg aaagctccat
     5101 gatgctctta cgcgaagtcc cttctaccag aatacgctgg gtggtgccga gcatccgacg
     5161 gctccatgcc atcgcttgct gattgatacg ttcttgcaga atatacagac gctgcttctt
     5221 ctcttcttcc ggaacatcat caaccatatc ggcggcaggc gttcctggac gtgcagagaa
     5281 gataaagctg tagctcatgt cgaaattgac gtcggcaatc agcttcatcg ttttctcgaa
     5341 gtcttcggtg gtttcgccag ggaagccaac aatgaagtca gaactgatct gaatatccgg
     5401 acgcgccgca cgcagtttac ggatgatcgc tttgtactcc agcgccgtat gggtacgtcc
     5461 catcaggttc agaatgcgat cggaaccgct ctgtaccggc agatgcagga agctcaccag
     5521 ctcaggcgtg tcgcggtaca cttcgatgat atcgtcggtg aattcgatcg gatggctggt
     5581 ggtaaagcga atacgatcga tcccgtcgat cgcagcaacc agacgcaaca gatcggcaaa
     5641 cgatccggtg gtgccgtcgt agttctcacc acgccaggca ttcacattct gaccgagcag
     5701 gttgacttca cgcacgccct gagccgcaag ctgggcaatc tcaaacagaa tatcgtcaga
     5761 cggacggctt acctcttcac cacgggtgta aggcaccacg cagtaggtgc aatatttatt
     5821 gcagccttcc atgatggaga caaacgcggt cggcccttcg gcgcgcggtt ccggcagacg
     5881 gtcaaacttc tcgatttccg ggaagctgat atctacaacc gggctgcggt cgccgcgcac
     5941 ggagttgatc atctccggca gacggtgcag cgtttgcggc ccaaaaataa tatcgacata
     6001 gtgggcgcgc tggcgaatgt gctcgccttc ttgcgatgcc acgcagccac cgacgccgat
     6061 aatcaggtct ggattcttct cttttaacag tttccagcga cccaactgat ggaagacttt
     6121 ttcctgagcc ttctcgcgga ttgagcaggt gttcagcagt agcacatccg cttcttccgc
     6181 cacgtcggtc agttgatagc cgtgggtggc atccagcaga tcggccatct tcgatgaatc
     6241 gtactcgttc atctgacagc cccaggtttt aatatggagt tttttggtca tcgacttgct
     6301 cttgcgaaat agtagccagg aatgcagggc gcatagtgta atgctttgct gccgttgtga
     6361 ccagtatgag cgttatcagc ccttgggggt aaaaatcctg taaacttaaa gcatattgct
     6421 aacaggatga ttgaccatga caaatcaacc aacggaaatt gccattgtcg gcggaggaat
     6481 ggtcggcggc gcactggcgc tggggctggc acagcacgga ttttcggtaa cggtgatcga
     6541 gcacgcagaa cctgcaccgt ttgtcgctga cagccaaccg gacgtgcgga tctcggcgat
     6601 cagcgcggct tcggtatcat tgcttaaagg gttaggggtc tgggatgcag tacaggctat
     6661 gcgttgccat ccttaccgca gactggaaac ctgggagtgg gaaacggcac atgtggtatt
     6721 tgacgcagcc gaattaaagt tgccgttgct tggctacatg gtggaaaaca cagtcctgca
     6781 acaggcgttg tggcaggcgc tggaagcgca tccgaaagta acgttacgtg tgccaacctc
     6841 gctgattgca ttacatcgag ataatgatct tcaggagctg gaactgaagg gcggtgaaac
     6901 gatccgcgcg aaactggtga ttggtgccga cggcgcaaat tctcaggtgc ggcagatggc
     6961 gggaattggc gttcatgcct ggcagtatgc gcagtcgtgc atgttgatta gcgtacagtg
     7021 tgagaacgat cccggcgaca gcacctggca gcaatttacc ccggacggac cgcgtgcgtt
     7081 tctgccgttg tttgataact gggcatcgct ggtgtggtat gactctccga cgcgtattcg
     7141 ccagttgcag aatatgaata tggcgcaact ccaggcggaa atcgcgaagc atttcccgtc
     7201 gcgtctgggt tacgttacac cgcttgccgc tggtgcgttt ccgctgacgc gccgacatgc
     7261 gttgcagtat gtgcagccgg ggcttgcgct ggtgggcgat gccgcgcata ccatccatcc
     7321 gctggcgggg cagggggtga atcttggtta tcgtgatgtc gatgccctga ttgatgttct
     7381 ggtgaatgcc cgcagctacg gtgaagcgtg ggccagttat cctgtcctca agcgttacca
     7441 gatgcggcgc atggcggata acttcattat gcaaagtggt atggatctgt tttatgccgg
     7501 attcagtaat aatctgccgc cgctgcgttt tgtgcgtaat ctcgggttaa tggcggcaga
     7561 gcgtgctggc gtgttgaaac gtcaggcgct gaaatatgcg ttagggttgt agccttacaa
     7621 cattgccgga tgcgtgccag ccgtaggtcg gataagacgc ggcagcgtcg catccgacat
     7681 tgaaggataa gacgtgtcaa catcgcattc gacattgaat gaacgcagaa aagcaaaaag
     7741 ctcgccgaag cgagcttttt taat
//
LOCUS       DAJTSI010000172         7741 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000172 DAJTSI010000000
VERSION     DAJTSI010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7741
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.172"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1013
                     /gene="ydfJ"
                     /locus_tag="OO327_003550"
     CDS             <1..1013
                     /gene="ydfJ"
                     /locus_tag="OO327_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HCR6019951.1"
                     /translation="LLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMGT
                     NCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEKV
                     NDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIP
                     TDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPST
                     IMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAGI
                     FCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPSR
                     SL"
     gene            1102..2562
                     /locus_tag="OO327_003551"
     CDS             1102..2562
                     /locus_tag="OO327_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR6019952.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="OO327_003552"
     CDS             complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="OO327_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR6019953.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2979..3665)
                     /gene="ydfH"
                     /locus_tag="OO327_003553"
     CDS             complement(2979..3665)
                     /gene="ydfH"
                     /locus_tag="OO327_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR6019954.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="OO327_003554"
     CDS             complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="OO327_003554"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR6019955.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4637..6682
                     /gene="dcp"
                     /locus_tag="OO327_003555"
     CDS             4637..6682
                     /gene="dcp"
                     /locus_tag="OO327_003555"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR6019956.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6727..7245)
                     /locus_tag="OO327_003556"
     CDS             complement(6727..7245)
                     /locus_tag="OO327_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCR6019957.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7521..>7741
                     /gene="ydeI"
                     /locus_tag="OO327_003557"
     CDS             7521..>7741
                     /gene="ydeI"
                     /locus_tag="OO327_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HCR6019958.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISH"
BASE COUNT         1939 a         1812 c         1955 g         2035 t
ORIGIN      
        1 tcttgctggt gacgctgcgt attattcagg gattgggtgc aggtgcggaa atttccggtg
       61 ccggtacgat gctggcggaa tatgcgccaa aaggtaagcg cggaattatc tcctcatttg
      121 tggctatggg aactaactgc ggcaccttaa gcgcaacggc aatctgggcc tttatgttct
      181 tcattctcag taaagaggaa ctgctggcgt ggggatggcg tataccgttc ctggcgagcg
      241 ttgtcgtgat ggtctttgct atctggttgc gtatgaatct gaaagaaagc ccggtctttg
      301 agaaggttaa cgacagcaac caaccgacag caaaacctgc acctgctggt agcatgttcc
      361 agagcaaatc cttctggctg gcaacagggc tgcgttttgg tcaggcgggt aactccggtt
      421 taattcagac tttccttgca ggctatttag tacagacgtt attgtttaac aaagcaattc
      481 caacagatgc attgatgatc agttcgattc tcggctttat gaccattccg ttccttggtt
      541 ggttatccga taaaattggt cgccggatcc cgtatattat tatgaatacc tctgcgattg
      601 tgctggcgtg gccaatgctt tctatcatcg tagataaaag ctatgccccg agcaccatta
      661 tggttgcact gattgtgatt cataactgtg cggtgctggg attatttgct ctggaaaaca
      721 ttaccatggc agaaatgttc ggctgtaaaa accgcttcac ccggatggct atttcaaaag
      781 aaattggtgg tcttatcgct tccggttttg gtcctatcct ggcgggtatt ttctgcacca
      841 tgacggaatc ctggtatccg atcgccatta tgatcatggc atattcagtg attggtttaa
      901 tctctgcgct gaaaatgcca gaggtgaaag accgtgattt aagtgcgctg gaagacgctg
      961 cggaagatca accgcgtgtt gtaagagctg cgcaaccttc cagaagtctg taaaccctca
     1021 atcccttctc ttaccggaga ggggattttt attcatataa caaaacatat agcttgccat
     1081 atttaaattt aaggaattgt tatgggaaat aatttgttat cagcaaaagc gacactccct
     1141 gtttatgatc gtaataacct ggccccaaga attgttcatt taggctttgg tgcatttcac
     1201 cgtgcgcatc agggcgtgta tgccgatatt cttgctacag aacatttcag tgactgggga
     1261 tattatgagg tcaacttaat cggcggcgaa cagcaaattg ccgatttaca acagcaagat
     1321 aatctttata ccgttgcgga aatgtcggcc gatgcgtgga cggctcgcgt cgttggcgtc
     1381 gttaaaaaag ccttgcacgt acagatggat ggcttagaaa ccgtgttggc tgcgatgtgt
     1441 gaaccgcaaa tcgcgattgt ctctctgaca atcaccgaaa aagggtattt ccactctccg
     1501 gcgaccggac agttaatgct cgagcacccg atggtcgctg ccgacgtgca aaatccccac
     1561 cagccgaaaa cagcaacagg ggtgattgtt gaggcgctgg ctcgccgtaa agcggcagga
     1621 cttcccgcat ttaccgtcat gtcatgtgac aacatgccag aaaacggtca tgttatgcgt
     1681 gacgttgtca cttcctacgc gcaagccgtt gatgtaaaac tggcacaatg gatcgaagat
     1741 aacgtgactt tcccatcaac aatggtggac cgtattgtgc ccgcagtgac agaggatacg
     1801 ctggcgaaaa tcgaacaact taccggtgtg cgcgatcctg ctggcgttgc ctgtgaacct
     1861 ttccgccagt gggttatcga agataacttt gtagccggac gtccggaatg ggaaaaagcg
     1921 ggagccgaac tggttagtga tgtgctgcct tatgaagaga tgaagttgcg catgctcaac
     1981 ggcagtcatt cattcctggc atatctgggt tatcttgctg gatatcagca cattaatgac
     2041 tgtatggaag atgaacatta tcgtcatgcg gcgtatggca tgatgttgca ggaacaagcg
     2101 ccgacgctga aagtgcaggg cgttgatttg caagattacg ctaaccgatt aattgcacgc
     2161 tatagcaacc cggcgttacg tcatcgaacc tggcagattg cgatggatgg cagccagaaa
     2221 ttgccacagc ggatgttgga ttctgttcgc tggcatctgg cgcatgacag caagttcgat
     2281 ctgctggcgc tgggcgtcgc gggttggatg cgttatgtcg gtggtgttga tgaacaggga
     2341 aatccgatag aaatcagtga cccactgtta cctgttattc agaaggctgt acaaagtagt
     2401 gccgaaggga aagcgcgcgt ccagtcattg ctggcgatta aggcaatctt tggtgatgat
     2461 ttgccagaca atagcttgtt tactgcaaaa gtgacggaag cgtacttgtc tttattagcg
     2521 catggcgcga aagcgaccgt ggcgaaatat tccgtgaagt aaaaaaagca atgccgccat
     2581 cggcggcatt cacacactta gttcatgccg agacgaatca ggtccagagg tgccagtttc
     2641 tcttcacaac cttcaacaac tacggttttt ccgcggcgga tttgctcagc ggtcagacct
     2701 tcagtatcaa tcgacaggat cttgccagtg tgaccggtgc cgctaaccat aacacggctg
     2761 ccagtggtga ttgcgttacg gttacgatcg taggtcgtca taggtgtttt ctcctttctg
     2821 atttacagtc gaagcccacg taacgggcgc aatataaata ctcctgtcga gtgaaatatt
     2881 ttttgttttt gatcaagctc acatcttttt tgatgcatgc atgagccgta aggcgggggg
     2941 aaaagcctct catcccggat gggatgagag taaatgaatt actcttcgct aaaccagtcg
     3001 ctgttttcct ggcggatctg gcgcacggat tcgctgattt cctgcaaatg ttgcgtcatt
     3061 gcacgttcta ccgcatcgcc atcacgtttt tgcagggcag agaaaatatc aagatgctgg
     3121 cgtaacagca tttctggtgg agaaacatgg tcgaaactca tatagcgcac gcgatcaacg
     3181 gtcgctttca ggttctcaat ggtatcccac gccagttgac agtcggcaat ctgcgtcagg
     3241 agttgatgga agttgtcatc aagttcaaaa aaatcatcca gttgcttgcg ctcaatggca
     3301 atgcgttgct ggtgaagatt ttgttccagt tggtagcact ggctttcggt aatcatgctc
     3361 gccgcccgac gcgccaccgc gcactcaatg gcctgacgga taaaactgcc gttgcgcacc
     3421 tgggccatgg aaattttgtt gacgtagctg ccacgctgcg gacgaatttg aatcaggccg
     3481 ttttccgcca gtttaataaa ggcttcacga accggctggc gtgacacatt gaaacgaaca
     3541 gaaacttctt tttccgacaa cggtgtgcct ggtgcaatca ggcaatggac aatgtcgcga
     3601 cgaagaatac gataaatctg ctgattgaca ggctgtgtgg gattaagttg cgtttcgacg
     3661 gtcattcgtt cttgcttgtg agtgagttaa ctgcatgagt ctaccacttt tttgtgacaa
     3721 gcaataaccc ggcagttacg ccgggtataa aaattactga cggtggacat tcagtccggc
     3781 atagctttgg gtaaccggca tcatttccag ggtattgata ttgacgtgag caggcagcgt
     3841 tgacacccac cagacggctt cgctgacatc ttctggcgtc aatgcaacgg tattttgata
     3901 ggttttttcc gctttaccgt catcgccttt aaagcggaca ttggaaaact cggtgccacc
     3961 caccagaccc ggttcgatgt cggtgacgcg caccgccgta ccatgcagat ccgtacgcag
     4021 attcaggcta aactgacgaa caaacgcttt cgtcgcaccg taaacgttac caccggcata
     4081 cggccagctg cctgccgttg agccaatgtt aataatatga ccatgattac gttcaaccat
     4141 acccggtaag acggcgcgcg tcatatatac taggcctttg ttgttggtat caatcatcgt
     4201 ttcccagtct tcaacgctgg ctttatgcgc aggctccatg cccaacgcca ggccggcatt
     4261 atttaccagg atatcaatat tgctccactc ggcagggagc gatgccagca tctcttcaat
     4321 agcggcgcgg ttgcgaacgt ccagttgggc gatatacaga ttatctccca gttcgtctgt
     4381 taactcctgc aaccgctcct ggcgacggcc agtggcgata actttatgcc cttgttgaat
     4441 aaaacgacga gtaatgcatt caccaaaacc tgccgttgct ccagttacta aaacgatcat
     4501 ctcactgttc ctcaacgctt ttgtgtgtca ataacatagc acgtgatgct taagagcggg
     4561 taatactggt ttgcaagatt gcactccgtt atcagtaagc ctacgctatg gaacacctcg
     4621 cgttcaggag aagaaaatga caacaatgaa tcctttcctt gtgcaaagca cactgccgta
     4681 tctggctcct cattttgatc aaattgccaa tcatcactat cgcccggcat tcgatgaggg
     4741 aatgcaacaa aagcgggcag aaattgctgc catcgcgctt aacccgcaaa cgcctgattt
     4801 caacaatact attctggcac tggaacaaag cggagaatta attacccgcg ttaccagcgt
     4861 cttttttgcg atgactgcgg cgcataccaa tgatgaatta cagcgtcttg atgaacagtt
     4921 ttccgctgaa ctggcggaac tggctaatga tatctatctg aacggtgaat tattcgcgcg
     4981 ggtagatgct gtctggcagc gccgtgaatc cctggggctt gatagtgaat ccatccgcct
     5041 ggtggaggtt attcatcaac gttttgtcct tgccggagcc aaacttgcac aagctgataa
     5101 agcaaaatta aaagtactga atacagaagc tgcgaccctg accagccagt ttaaccagcg
     5161 gttacttgca gcaaataaat ccggcggtct ggttgtgaac gatatcgcgc agctggcagg
     5221 aatgagtgag caagagattg cgctggcggc agaggcggct cgcgagaaag gtctggataa
     5281 caaatggctg attccgctgc tgaataccac ccaacaaccg gcgcttgccg aaatgcgcga
     5341 tcgtgcgacg cgtgaaaaac tgtttattgc gggctggacg cgagcggaaa aaaatgatgc
     5401 caatgatacc cgcgctatca ttcaacgtct ggtagagatt cgcgcacagc aggcgaaact
     5461 gcttggtttt cctcattatg ccgcatggaa aatcgccgat cagatggcaa aaacgccaga
     5521 agcagcactc aactttatgc gggaaattgt tccagcggcg cgtcaacgtg cgagcgatga
     5581 attagcctcc atacaggcgg ttatcgataa gcagcagggc gggtttagcg cgcagccgtg
     5641 ggactgggca ttttatgcgg aacaggtacg gcgggagaaa tttgatcttg atgaggcgca
     5701 gctcaagcca tattttgaat taaacacggt gttaaatgaa ggtgtattct ggaccgcgaa
     5761 tcagctcttc ggtattaagt ttgtcgaacg ttttgatatt ccggtctacc atcctgacgt
     5821 tcgtgtgtgg gaaatttttg atcataatgg cgtgggactg gcgttatttt acggtgattt
     5881 cttcgcccgt gattcaaaaa gcggcggtgc atggatgggc aattttgttg agcaatcaac
     5941 gcttaatgaa acgcatccgg taatttataa cgtctgcaat tatcagaaac ccgctgccgg
     6001 tgagcctgcg ttgttactct gggatgatgt aataacctta ttccatgaat ttggtcatac
     6061 gctgcacggc ctttttgccc gccagcgtta tgccacgctt tccggcacca acacgccgcg
     6121 tgattttgtc gaatttccgt cgcaaatcaa cgaacactgg gcaacgcatc cgcaggtatt
     6181 cgctcgctac gcccggcatt atcagagcgg ggcagcaatg cctgacgaac tgcaacagaa
     6241 aatgcgtaat gccagcctgt tcaacaaagg gtatgagatg agcgaactgc ttagcgccgc
     6301 acttctcgat atgcgctggc attgcctgga agaaaacgaa gcaatgcagg atgtcgatga
     6361 ttttgaattg cgggcgctgg tggcggaaaa tatggatctt cctgctatac cgccacgcta
     6421 tcgcagcagt tatttcgccc atatttttgg tggcggatat gctgcaggtt attacgctta
     6481 tctgtggacg caaatgttgg ccgatgatgg ttaccagtgg tttgttgagc agggcggatt
     6541 aacgcgtgaa aatgggcagc gttttcgcga ggcgatcctt tccagaggta acagcgaaga
     6601 tctggaacgc ctgcatcgac aatggcgtgg taaggctcct cagattatgc cgatgctgca
     6661 acatcgtggc ttgaacatat aaacaccacc aatggctggc ctggagatat cgccaggcca
     6721 gcctggttat agcagtaatt gcagcagctg ggcgagggca aacctcaccg ctaaagctaa
     6781 taccgtttcg cagtcgccag caaaatgcat aaccgcagtg tagttctggc ctttaatatg
     6841 ccacgcgaac cagacggtac ccgctggcgt accatcttca ccgccctccg gtccgccgta
     6901 gccggtaatg gcaatactga catcagcatc cgcacgctct atggcacctg ttgccatttc
     6961 tgctgccact ttctcactca ccgcagaata tcgttcaaga gactgctggc ttacgctgag
     7021 gattttcatc tttgcctgat cggtgaaagt aacaaagcct gcaccgtaaa atttgggtgt
     7081 atcttcagct gcacacaggg cgctagccaa cttaccgccg gtgcatgatt cggcggtagt
     7141 taagcgtaat tgatccgcga taagtctttg actcaacgcg gatgtcaggt tttcaatagt
     7201 gtcggtatct gcgagttgta caattttatc tttattaatg ttcactgttt tttgtccacg
     7261 ctattatatg taaaggtgat ttgctttttc tgtatgctcg tccaatataa taattttcaa
     7321 acatataaat ccgattattc tgaaaacatg tttaagactc ttcaatcttc ttaactttag
     7381 tgagtcaaat tctctgaata tttgacagct agtgggctga ttttaaatgc cttataacaa
     7441 ttggcagtat tcttatttgg caattataaa aatagcaacc agacttaata agtcaaacca
     7501 aacgcaatta aggagttagt atgaaatttc agtccattgt attagcaagt tttcttgtca
     7561 tgccttatgc gttggcagat gaccagggcg gtttaaaaca ggatgcagcg ccaccgccgc
     7621 ctcatgcaat agaagatggc tatcgcggaa ccgatgatgc aaaaaaaatg accgttgatt
     7681 tcgcaaaaaa catgcacgat ggcgcttcgg tttcactacg cggtaatttg atttctcaca
     7741 a
//
LOCUS       DAJTSI010000173         7565 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000173 DAJTSI010000000
VERSION     DAJTSI010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7565
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.173"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(88..1715)
                     /gene="yeeO"
                     /locus_tag="OO327_003558"
                     /pseudo
     CDS             complement(88..1715)
                     /gene="yeeO"
                     /locus_tag="OO327_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            1720..1795
                     /locus_tag="OO327_003559"
     tRNA            1720..1795
                     /locus_tag="OO327_003559"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1753..1755,aa:Asn,seq:gtt)
     gene            complement(1833..2783)
                     /gene="cbl"
                     /locus_tag="OO327_003560"
     CDS             complement(1833..2783)
                     /gene="cbl"
                     /locus_tag="OO327_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR6019959.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(2885..3802)
                     /gene="nac"
                     /locus_tag="OO327_003561"
     CDS             complement(2885..3802)
                     /gene="nac"
                     /locus_tag="OO327_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR6019960.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4130..4205
                     /locus_tag="OO327_003562"
     tRNA            4130..4205
                     /locus_tag="OO327_003562"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4163..4165,aa:Asn,seq:gtt)
     gene            complement(4261..5196)
                     /gene="ldtA"
                     /locus_tag="OO327_003563"
     CDS             complement(4261..5196)
                     /gene="ldtA"
                     /locus_tag="OO327_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6019961.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(5258..6337)
                     /gene="cobT"
                     /locus_tag="OO327_003564"
     CDS             complement(5258..6337)
                     /gene="cobT"
                     /locus_tag="OO327_003564"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR6019962.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6349..7092)
                     /gene="cobS"
                     /locus_tag="OO327_003565"
     CDS             complement(6349..7092)
                     /gene="cobS"
                     /locus_tag="OO327_003565"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR6019963.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7089..>7565)
                     /gene="cobU"
                     /locus_tag="OO327_003566"
     CDS             complement(7089..>7565)
                     /gene="cobU"
                     /locus_tag="OO327_003566"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCR6019964.1"
                     /translation="SSQVLYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDEL
                     IHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQR
                     CPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         2086 a         2048 c         1639 g         1792 t
ORIGIN      
        1 ggcgaatatt agcgatgccc acccacaatg tcaaagcctg ttttttaaat ttgaaatcgt
       61 ttgctgaaat aatctgcatt ttgtcgttta ttccgacaca actggctttt tttcacactt
      121 ttgcggctcg gatcgagggt atttccacag ccaacgtcca gtaaccattc gccagtaaaa
      181 cagcacggcc cgcacagccc agtcggcaaa cattcccatc cagacaccaa ccacacccca
      241 gccaagcatg attccctgca cataaccgac tacaacccgg caaccccaca tgctcaacat
      301 agaaacccac atggcgtaac gggcatcacg agcaccctta aatccagcgg gtagcaccca
      361 tgaggcggac caaataggca taaataaagc atttagctaa atcagaatca caacgacgtg
      421 tttaacctgt ggatcctggg tgtaaaacga tgccataacc ccggcaaagg gagccgttag
      481 ccaggcgatg gccgttaatc caagagtgga cagccagaac acatgccgca actgaatctc
      541 tgcttgcgct atctgcccta cccccaacct tcggcctgta atgatcgtag aagcagaacc
      601 gagcgcactt ccgggtaagt tgataagagc cgcaattgag aacgcgataa aatttccggc
      661 aataacgctg gtccccatcc cggcaacgaa catttgggtt aataaccgac cactggtaaa
      721 taacactgat tcgacactcg ctggaatacc aatccccatg acttcccaga taatgctaaa
      781 attcagcggt ttaaaatagc tctttaacga aatccttagc gcaggattaa aaccaatcgc
      841 cagcacccac aaaattgcaa ctgcgccaat ataacgagaa atggttaaac ccaacccagc
      901 cccgaccagg agaaaaggcc gtaaatcaat atgccgctaa taataatatt aagaatattc
      961 aggctaccgt taatcagtag cggtattttc gtattccctg caccacgaag tgccccgcta
     1021 ccaataagag tgatggcagc ggctggataa ctgagtacgg tcagctccag ataagtcaac
     1081 gccagtgctt taacttctgt cgtggcatca cccgcgacga aatcaataat ttgttcgcca
     1141 aaatgatgaa ttagcgttgc caacagtacg gcaaacaacg tcatgatcac caatgactgc
     1201 cgcgtcgcca ccctcgctcg tcgtcgatcc cgcttaccga gactaaatgc tacaacgaca
     1261 gtagtaccaa gatcgatagc tgcaaaaaaa gccataatga ccatattgaa gctgtccgcc
     1321 aaccccacgc cggccatcgc atcttttccc agccaactga ccagaaaagt gctcaggacc
     1381 cccatcaaca ggacacaggc gttctccatg aagataggaa cagcaagcgg ggttatctcg
     1441 cgctagaaca acactttgta gctcttgcgt ttagagtgcc agcgagtgcc gtgaacaacc
     1501 tggcgtaaag cagaggagat attcaaagcc gaccttaatt gcagaaagtg aaaccacatt
     1561 tcaaatgatg agggagaatc agcaaagctg caaagatttt cgccaacaaa ttgtctgcaa
     1621 atgcaacaaa ctgttgatag aaacggcaaa cagttgggaa atttaaaaat cgggtttgac
     1681 aaaatatttt tcgccgttaa gatgtgcctc aacagcgatt cctctgtagt tcagtcggta
     1741 gaacggcgga ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattt
     1801 ctgaaaagcc cgcttttata gcgggctttt tgctatatct gataatcaat ttcctcttca
     1861 ctgttttcca tcacctggcg cttgatatcc tcaactgaca gtcctgcatt acaaagttcc
     1921 agaaagcgcc aaacatagtt acgctgaagt tgtcctcgct tcagtcccaa ccagacagta
     1981 ttagcatcga aaagatgccg cgtatccagg cggattaaat tctcttcctc ttgttcgccg
     2041 ctggattgtt cggcaactaa tccgatccca agcccaagag caacataggt tttaatgaca
     2101 tcagaatcct gtgcacttaa tacaatatct gccggcaaac ctttgcgggt aaatgcgtca
     2161 tcagtgcgtg agcgccccgt aattccctgt cggtaagtaa ttaacggcca cttcgctatt
     2221 gattccagcg tcaatggtgt aatttgcgtt aagggatgat cgagtggaac aagcaaacta
     2281 tggtgccaac aaaaccatgg gaaggcgacg agctgcgggt cattactcaa acgctcgctg
     2341 gcgataccaa tatcagcttc gccattttgc aacaacgtcg caatttcctg tggcgaaccc
     2401 tggattagct cgagccgaac ctccgggaaa agttcgcgaa aagctttaat gacctctggc
     2461 aagctataac gtgcctgagt atgcgtcgtt gcaatagtga gaacaccaga cgtatcgttg
     2521 gtaaacaggt ctgcaagccg acgaacatta ctggcttcat tcagaatacg ttctgcaatg
     2581 accagtaatg ctttgcccgg ttcagtcatc cccagcagtc gcttacctcg tcgaacaaat
     2641 atttcgatgc caaattcatc ctccagttcc cgaatatgac ggctaacgcc tgactgtgag
     2701 gtaaaaagca tattcgcaac ctctgtcagg ttgtaatcct gacgtgcagc ctcgcggatt
     2761 atctttagtt gttggaaatt cacggtaaac tccgggcagt tcagatatcc cgttattgtt
     2821 aaagtctaat gcccggcata acaaataata aaaacccgca tcttattcca tcccgatata
     2881 acgcttagct caccaattgc cactgccttt tttccatcac tggagaacta atcactgaca
     2941 ttaacaactc tttcactgcc tgtgcctgtg gtgacaagtt tgctctggcg ggtaaattta
     3001 aggacaaaga gagactcatt gaaggagtgg taatgcgtga catccaccca tttactgcgc
     3061 cacataacga acgcgcagcc gattcaggta atactgcaac gcccatgccg ctggcaatcg
     3121 ctgcggtaag cgtggcaata gactcaattt caccaatgac ttttgccgtg agccgccgta
     3181 gggaaaaagc ctcatcaaca cgaagtctaa cagcgctgta atcactgggg agaaaaaggt
     3241 tcatttgcgc aatagcatta acatcaacgt tttgccccgg gcaatcttga gttcctacca
     3301 ggaaaagatc ttctttcagc aaagcctgac tggatacccc agccacaggg gaatgctcat
     3361 aaatcaccgc catatcgagt tggtgattta tcagtttttc gttaagcact gcaccactat
     3421 tttcatgaag atagataacg acctccggga attcagcgcg aaccgcctgt aataagggca
     3481 tggtgatgga tgacgcagcg gttcctggcg caaagccaat cgagacttgc cccgataatg
     3541 actgaccaac gttatgcacc gccagttggg cctgctcaca ctgacgtaaa atggcccgtg
     3601 catgggtata gagaattttt ccggcgtctg ttggcgtaac gccccgtttt gtgcgaatca
     3661 aaagctgttg atttaactca ccttccagta tggcaacctg ttggctgagc gctggttgtg
     3721 cgatatgcaa tacttcagca gcctgggtca ggctaccaat atctacaatt tttacgaagt
     3781 atttcaggcg tctgaagttc atgttgcctc cggtttttaa gaatcggccc aagtgccgcc
     3841 attacttaca accagtatgc aagatgcctg ccagttctat tttggcgttg atgcgcaagc
     3901 taaccaactg tcaaataaga gattatgata gattcgtcat tggccctttt tatcagcagt
     3961 cgcattcacc tgccctataa aaggagggta tgcaccacga tggttcatta cccaataaga
     4021 ttgaaagctc accactttgt tgaaattggc agcaaacaaa caaaaaaaat gcatttcacc
     4081 ctttgacatc accatgcact gccattaata tgcgccccgt tcacacgatt cctctgtagt
     4141 tcagtcggta gaacggcgga ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg
     4201 agccaaattc aaaaaagcct gctttcgagc aggcttttta ctttctaatt accaacgctc
     4261 ttaaaacatc tgtcttgaac cagaactaat ttgcacaggc attcccgatc gatgttgcaa
     4321 cgcagcattt gcgcgattta catcaacttc ttgcccgttg ataaacgccc gcaaagatgg
     4381 ggttaccggc aatggcactt ttcggtcaga ctcatattct gcacgattgc gcgacagcgg
     4441 ctcatgaact tccagccact ttgaaccatc cggttccgtt gtgtatttca ctggctgatc
     4501 aataatttgt acacgagtgc ctacaggaac attatcaaac agatatttga tatcgtcatt
     4561 acgcagacga atacagccct gacttacacg gagcccaata ccaaaattgg cattggtacc
     4621 gtggatggca tacaacctgc caatataaat cgcgtacagc cccatggggt tatcgggccc
     4681 cgcaggaaca aatgcgggca aactctcccc tcgtttcgca tattcgcgcc ggatgttcgg
     4741 cgttggcgtc caggttggtg cttcttgttt acgttcaacg gtagtcaccc agttacgcgg
     4801 ggtttctcgc ccagcctggc cgataccaat aggaaagact tccactgtat tactgtctgg
     4861 cggatagtaa taaagacgca tttcagcgac gttaaccaca atccctttac gaacagtggc
     4921 gggcaaaatc agttgctgcg gaatggtgag ttgtgagcca ggcttcggca aaaaaacatc
     4981 agcgcccgga ttcgcttcca gcatattact taacccttgc ccgtattgtg cggcaaaagt
     5041 ctccagcggc tgagtattgt gatcaggaac agttacagta agcgactgcc ccactaaacg
     5101 gctaccctct ggaggtaatg gataagttac cgccaggcta ttttggctgg caaaaagcag
     5161 agcaaatgag caaagaatat ttacacgacg catcatgtcc ctttcctaag tcgcgaatac
     5221 tatccgataa gtatagcttt tatcagactt ttcgttttta gctgttcaaa tcagaagtcg
     5281 tattccccgg tagaacaata ttactggcag caagttcgcc catgttgttg tatatcgcac
     5341 aagcagcttc gatgatggac atcgccagag ctgcaccact cccctcacct aaacgcatat
     5401 ccatattgag ataaggctcc agccccaaat gcgagagcgc tatacgcgcg cctttttctg
     5461 ccgacaagtg agaaggaatg agatacggtt tgattgcagg agacatctgg caggctgcga
     5521 gcgcagcagc ataagaaaga aatccatcca gcaacacggg taaaccgcag gaagcagcac
     5581 ctaacatcac tccagctatt ccgaccaaat caaatccacc cacttttgcc aggacatcaa
     5641 caccatcctg aggatttggt tgattcaacg taatcgcccg acgcacaaca tcaattttat
     5701 tagccagttt atctgtcggc aggtttgcgc caatcccaac cacttcttca ggatcccggc
     5761 cagtgattgt gctgactatt gccgctgccg gtgtcgtgtt tgccatcccc agttcaccta
     5821 caccaaacag cgtgacaccg tttttcgcca gctcccgcgt ataacatatg acgtccaaaa
     5881 gcaacttttc agcctgacgg cgactcattg ccggagctga agcaatattg ccgctacctc
     5941 gtgcgacacg catgttgata agcccgggga taggctcagc agtatcaata ccaacatcaa
     6001 ttacgtggac gttagcgccc gcttgtgctg ccagcacaca cacgccggtt gttccacggg
     6061 tcatattttc agcctgtatg gctgtcactt cttttggaga aatagcgacc ccttcctccc
     6121 agacgccgtg atcggcacac ataaccagta ccgctttttt gcccacatgc ggtatgccat
     6181 tcacccccgg cattcctgcc agttgtatgg caagcgcctc cagctttcct aggctaccaa
     6241 caggtttgaa taacccgtca acatgccgtt gtgcacgcga catagcggca gaattgatgg
     6301 ctgggatcgt attcagtaaa tcggcaatta tttgcatgtc acgtttcttt atagcagtgc
     6361 cagcaggaag accagttcac caagctctat ggctgctccc agcgtatcgc ccgtttgccc
     6421 gcccagcgta cgtttgagaa gctgaccgag gatgaaaatc gccaccatcg tcaccaccat
     6481 agcagccaca ccatgcatac ccagcaataa tacggcagca aaaatagcgg ctaagccgag
     6541 tgtgacgcag gtttgtcgcc catcaatttt gccaatgaac acattgccaa gcccttcttc
     6601 ccgcgcataa cgatgacgat acatcaataa tgcggcagtc ccgcggctga ccgcacatgc
     6661 tgccgccagc gatgcaagga tcggttcgcc acgcagagcc aactcgctta acaccagaat
     6721 ctttgccagt accacaaaaa tcaatgccag accgccgtgg gtgcctaaac ggctatcacg
     6781 catgatttcc aacatgcgat cgcgactacg tgcagaaaat acgccatcac aggtatcagc
     6841 gaggccgtcc agatggaatt ccccggtcat cagcgccagc acaagtacgc taaacagtgc
     6901 cgccagtggt gcgccacacc atgcctgcag caccatgaag accagcccgc taatcgcgcc
     6961 aagcaataat ccaatcaaag gaaaagtaat aataccgcga gaataatgtt cgaaatccag
     7021 tccctgggac cagcgacgcg gtacaggcag acgcgtaata aatgagagca tcgcccaaaa
     7081 taatttgctc atttgatttt tactccaata cccgaaacca ccagccatac ttcatttgcc
     7141 gcagcggcca actgctgatt tacccgcccg gcaatatcac gaaaatgtcg tgccagacga
     7201 ctctccggca caatccccat tcccacttcg ttagtcacta atacaacctt tgcggggcaa
     7261 cgttggcagg cagcaatcaa cgactgaatc tcagcattaa tcgcctgttc catcgcctga
     7321 taatcccatt catcagggtc tttatcgccg ccataatcaa acaacagatt agtcaccatt
     7381 gtggtaacgc attccagcaa cacagcctca ttcgggttaa tgtctgcatg aattaattca
     7441 tcaagatgtt gccagcgctc cactgtgcgc cagtgctccg ggcggctttg ccgatgatgt
     7501 tctatccgtg cagccatctc atcatcaagg atttgcgagg tagcgatata cagaacctgt
     7561 gaaga
//
LOCUS       DAJTSI010000174         7473 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000174 DAJTSI010000000
VERSION     DAJTSI010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.174"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1158
                     /gene="rbsA"
                     /locus_tag="OO327_003567"
                     /pseudo
     CDS             1..1158
                     /gene="rbsA"
                     /locus_tag="OO327_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1163..2128
                     /gene="rbsC"
                     /locus_tag="OO327_003568"
     CDS             1163..2128
                     /gene="rbsC"
                     /locus_tag="OO327_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR6019965.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2153..3043
                     /gene="rbsB"
                     /locus_tag="OO327_003569"
                     /pseudo
     CDS             2153..3043
                     /gene="rbsB"
                     /locus_tag="OO327_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3169..4098
                     /gene="rbsK"
                     /locus_tag="OO327_003570"
     CDS             3169..4098
                     /gene="rbsK"
                     /locus_tag="OO327_003570"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR6019966.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4102..5094
                     /gene="rbsR"
                     /locus_tag="OO327_003571"
     CDS             4102..5094
                     /gene="rbsR"
                     /locus_tag="OO327_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR6019967.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="OO327_003572"
     CDS             complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="OO327_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR6019968.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYAREHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6510..7202)
                     /locus_tag="OO327_003573"
     CDS             complement(6510..7202)
                     /locus_tag="OO327_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR6019969.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1898 a         1810 c         1969 g         1796 t
ORIGIN      
        1 aaccatgtat gtcgaagcgg ataaattgct ggctaaactt aacctgcgct ttaaaagcga
       61 caagttagtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg ccaaagtgct
      121 gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga ccgataccga
      181 aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccact gtattgtcta
      241 tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg tttttcgtga
      301 tgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc tgattgagat
      361 gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc caggagatat
      421 ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt ttactttacg
      481 caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg aactgatgaa
      541 agtgctctac ggcgcactgc cgcgcaccag cggttacgtc accctggatg ggcatgaagt
      601 cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct ccgaagaccg
      661 taaacgtgac ggtttagtgc tgggcatgtc agtaaaagag aacatgtcgc tgacagcgct
      721 gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc aggctgtgag
      781 tgatttcatt cgtctgttta atgtgaaaac gccgtcgatg gaacaggcaa ttggtctgct
      841 ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac ccccaaagta
      901 ttgatccttg atgagcctac ccgtggcgta gatgtcggcg cgaaaaaaga gatctatcaa
      961 ctgattaacc agttcaaagc cgatggcttg agtatcattc tggtgtcatc ggagatgcca
     1021 gaagtattag gcatgagcga tcgcatcatc gtcatgcatg aagggcatct cagcggggaa
     1081 tttactcgtg agcaggccac ccaggaagtg ttaatggctg tcgctgtggg caagcttaat
     1141 cgcgtgaatc aggagtaaaa aaatgataac ccagactgtc tctggtcgcc gttatttcac
     1201 gaaagcgtgg ctgatggagc agaaatcgct tatcgctctg ctggtgctga tcgcgattgt
     1261 ctcgacgtta agcccgaact ttttcaccat caataactta ttcaatattc tccagcaaac
     1321 ttcggtgaac gccattatgg cggtcgggat gacgctggtg atcctgacgt cgggcataga
     1381 cttgtcggta ggttctctgt tggcgctaac cggcgcagtt gctgcatcta tcgtcggcat
     1441 tgaagtcaat gcgctggtgg ctgtcgctgc tgctctcacg ttaggtgccg caattggtgc
     1501 ggtaaccggg gtgattgtag cgaaaggtcg cgtccaggcg tttatcgcta cgctggttat
     1561 gatgctttta ctgcgcggcg tgaccatggt ttataccaac ggtagcccag tgaataccgg
     1621 ctttactgag aacgccgatc tgtttggctg gtttggtatt ggtcgtccgc tgggcgtacc
     1681 gacgccagtc tggatcatgg ggattgtctt cctcgcggcc tggtacatgc tgcatcacac
     1741 gcgtctgggg cgttacatct acgcgctggg cggcaacgaa gcggcaacgc gtctttctgg
     1801 tatcaacgtc aataaaatca aaatcatcgt ctattctctt tgtggtctgc tggcatcgct
     1861 ggccgggatc attgaagtgg cgcgtctctc ctctgcacaa cccacggcgg ggactggcta
     1921 tgagctggat gctattgctg cggtggttct gggcggtacg agtctggcgg gcggaaaagg
     1981 tcgcattgtt gggacgttga tcggcgcatt aattcttggc ttccttaata atggattgaa
     2041 tttgttaggt gtttcctcct attaccagat gatcgtcaaa gcggtggtga ttttgctggc
     2101 ggtgctggta gacaacaaaa agcagtaata acgactacag gacatcttga atatgaacat
     2161 gaaaaaactg gctaccctgg tttccgctgt tgcgctaagc gccaccgtca gtgcgaatgc
     2221 gatggcaaaa gacaccatcg cgctggtggt ctccacgctt aacaacccgt tctttgtatc
     2281 gctgaaagat ggcgcgcaga aagaggcgga taaacttggc tataacctgg tggtgctgga
     2341 ctcccagaac aacccggcga aagagctggc gaacgtgcag gacttaaccg ttcgcggcac
     2401 caaaattctg ctgattaacc cgaccgactc cgacgcagtg ggtaatgctg tgaagatggc
     2461 taactaggcg aacattccgg ttatcactct tgaccgccag gcaacgaaag gtgaagtggt
     2521 gagccacatt gcatctgata acgtactggg cggcaaaatc gccggtgatt acatcgcgaa
     2581 gaaagcgggt gaaggtgcca aagttatcga gctgcaaggc attgccggta catccgcagc
     2641 ccgtgaacgt ggcgaaggct tccagctggc cgttgctgct cacaagttta atgttcttgc
     2701 cagccagcca gcagattttg atcgcaccaa aggtttgaac gtaatgcaga acctgttgac
     2761 cgctcatccg gatgttcagg ctgtattcgc gcagaatgat gaaatggcgc tgggcgcgct
     2821 gcgcgcactg caaactgccg gtaaatcgga tgtgatggtc gtcggatttg acggtacacc
     2881 ggatggcgaa aaagcggtga atgatggcaa actagcagcg actatcgctc agctacccga
     2941 tcagattggc gcgaaaggcg ttgaaaccgc agataaagtg ctgaaaggcg agaaagttca
     3001 ggctaagtat ccggttgatc tgaaactggt tgttaagcag tagttttaat caggttgtat
     3061 gacctgatgg tgacataaat acgtcatcga cagatgaacg tgtaatataa agaaaagcag
     3121 ggcacgcgcc accctaacac ggtggcgcat tttatggaca tcccgaatat gcaaaacgca
     3181 ggcagcctcg ttgttcttgg cagcattaat gctgaccaca ttcttaatct tcaatctttt
     3241 cctactccag gcgaaaccgt aaccggtaac cactatcagg ttgcatttgg cggcaaaggc
     3301 gcgaatcagg ctgtggctgc tgggcgtagc ggtgcgaata tcgcgtttat tgcctgtact
     3361 ggcgatgaca gcattggcga gagcgttcgc cagcagctcg ccactgataa catcgatatt
     3421 actccggtca gcgtgatcaa aggcgaatca acaggtgtgg cgctgatttt tgttaatggc
     3481 gaaggtgaga atgtcatcgg tattcatgcc ggcgctaatg ctgctctttc cccatcgctg
     3541 gtggaagcgc aacgtgagcg tattgccaac gcgtcagcgt tattaatgca gctggaatca
     3601 ccactcgaaa gtgtgatggc agcggcgaaa atcgcccatc aaaataagac tatcgttgcg
     3661 cttaacccgg ctccggctcg cgaacttcct gacgaactgc tggcgctggt ggacattatt
     3721 acgccaaacg aaacggaagc agaaaagctc accggtattc gtgttgaaaa tgatgaagat
     3781 gcagcgaagg cggcgcaggt actgcatgaa aagggtatcc gtactgtact gattacttta
     3841 ggaagtcgtg gtgtatgggc tagcgtgaat ggtgaaggtc agcgcgttcc tggattccgg
     3901 gtgcaggctg tcgataccat tgctgccgga gataccttta acggcgcgtt aatcacggcg
     3961 ttgctggaag aaaaaccatt gccagaggcg attcggtttg cccatgctgc cgctgcgatt
     4021 gccgtaacac gtaaaggcgc acaaccttcc gtaccgtggc gtgaagagat cgacgcattt
     4081 ttagacaggc agaggtgacg cttggctaca atgaaagatg ttgcccgttt ggcgggcgtt
     4141 tctacctcaa cagtttctca cgttatcaat aaagatcgct tcgtcagtga agcgattacc
     4201 gccaaagttg aagcggcgat taaagaactc aattacgcgc catcagctct ggcgcgtagc
     4261 ctcaaactca atcaaacaca taccattggc atgttgatca ctgccagtac caatcctttc
     4321 tattcagaac tggtgcgtgg cgttgaacgc agctgcttcg aacgcggtta tagtctcgtc
     4381 ctttgcaata ccgaaggcga tgaacagcgg atgaatcgca atctggaaac gctgatgcaa
     4441 aaacgcgttg atggcttact gttactgtgt accgaaacgc atcagccttc gcgtgaaatc
     4501 atgcaacgtt atccgacagt gcctaccgtg atgatggact gggctccgtt cgatggcgac
     4561 agcgatctta ttcaggataa ctcgttgctg ggcggagact tagcaacgca atatctgatc
     4621 gataaaggtc atacccgtat cgcctgtatt accggtccgc tggataaaac tccggcgcgc
     4681 ctgcggttgg aaggttatcg ggcggcgatg aaacgtgcgg gtctcaacat tcctgatggc
     4741 tatgaagtca ctggtgattt tgaatttaac ggcgggtttg acgccatgcg ccaactgtta
     4801 tcacatccgc tgcgtcctca ggccgtcttt accggaaatg acgctatggc tgttggcgtt
     4861 taccaggcgt tatatcaggc agagttacag gttccgcagg atatcgcggt gattggctat
     4921 gacgatatcg aactggcaag ctttatgacg ccaccattaa ccactatcca ccaaccgaaa
     4981 gatgaactgg gggagctggc gattgatgta ctcatccatc ggataaccca gccgaccctt
     5041 cagcaacaac gattacaact tactccgatt ctgatggaac gcggttcggc atagatttac
     5101 gctgtctttt gatcaaatta ttaccatcgg ttgttttcag aagcatgaac attgctgctg
     5161 aagcaacagt aataatgccc atcgtgataa acgtatagtg gaattgttcg acagtcgttg
     5221 tgccttccat cccttcataa atgcgaagga cggccgcact tacagcaacg cctaaactaa
     5281 tcgacagttg ctgcgtgacc gccagaacac tgttaccgct gctggcgttg tcatcggtca
     5341 gatcggcaag tgtgatggta ttcatcgcgg taaactgcgt cgacatagcc atccctaata
     5401 taaacaacgg caagatcagc atccatatcg ccattgccgg tgattgcaaa gagaactgag
     5461 cgatcattag cccaataatc accgtgatcc ccactaatgt atggcgatag cccagacgac
     5521 gtaagacttg ggtaaccatc gattttgcaa taatggaacc taacgctgtc ggtgccatca
     5581 tacagccggc aataaacgcc tgataaccaa atcctacctg taacatcaat ggcataagga
     5641 acggtacaca gccggtcccc agacgggttg caatattgcc tacgataccg atcgagaaag
     5701 tgcgggtttt aaataaatct aatgaaatta atgggtttgg cgtacgtcgc gcatggagaa
     5761 tatagagaag cagtaacccg atgctggtga caattaccgt caaggcaatc cagctggcga
     5821 caatcttttc cccgaatagc tctattccgc ttgaaaagag aacaaggctg aggccaaaca
     5881 gcaaaaagcc agtgatatcg aatctgcgtc gtgcggtggt gaaattgggc atatgttcgc
     5941 gcgcgtaaag aaggcccgca atacctatgg ggatattaat taaaaatatc cagtgccagg
     6001 ttgcccaggt gaccagcacg ccgccaagaa cggggcctaa aattggcccc accagacccg
     6061 gcatggcgac aaaattcaat accggaagaa gttcattacg aggataagca cgcagtaagg
     6121 ccggccgagc aacaggcatc atcattgcgt cgcctatccc ttgaataacc cggaagacaa
     6181 ccagctgtgg tagcgaatta gaaagtgcgc aggccagaga acccaacgtg aacagactca
     6241 cggcaagggt aaaaatgcga cgcgtaccga agcgatcggc tagccatccg cttaccggaa
     6301 taagcatcgc caccgtcagc gtataactga tgatggctga ttgcatcgcg agaggagaac
     6361 gattaaggct atgagcgatt gcgggtaagg cggtattcag aatagtggca tcaagtgcct
     6421 gcatgaagaa ggccatcgcc gcgatccacg gcaaacccga catactacgc ttctttttat
     6481 cgctcattca atgtcctgtt atcgggttat cacttatcag gtgagcgtag cagcgcctga
     6541 caagctttaa atgccgcgtc gccatcgctt tggataatcg catcgacaat cgcctggtgc
     6601 agatccagct ttatcactgt gtcgctggta attgacgtga agtaagtgtg ataaaccgaa
     6661 tggaatagcg aggcgaatga ggtcaaaaac ggattggcgc tcatttcata gatatgctca
     6721 tgccaggcca tatcgacttc gatccagcgt tcacggcgaa agttctcttt taatgccgcc
     6781 atttcggcca ttaacgtatt gagatgcgcc ttctgttccg cggtgccaac cgttgctgcc
     6841 agtaggcagg cttgcggctc cagacagata cgcataacca gaaagtgatc gatgacctga
     6901 tgaaagttct cttctgttat ccaccaagta agcaattcct gatcaagaaa attccagttc
     6961 gattgtggca tgacccgagt accaattcgc ggtcgcggta aaaccatccc ttttgccgtt
     7021 aacgttttga ccgcttcgcg taccgctgta cgactcactc caaattgctc gcccagctca
     7081 atctcaccgg gcaaaatggt gccgggttca tattcacctt ttaagatccg ttgcgccagc
     7141 ttctcagcca gaacatacga aaggtttttc tgtgcagcta actgttgtgc gcttaaaggc
     7201 attacttatc ttcctttttc tttttattcc tccttagtat gccaccagga agtgtgatta
     7261 cggttgcaaa aacggcaaat tgcttgtttt atggcacatt aacggggtgt tttgctgaaa
     7321 aaatgcgcgg tcagaaaatt attttaaatt tcctcttgtc aggccgggat aactccctat
     7381 aatacgccac cactgacacg gaacaacggc aaatacgccg ccgggtcagc ggggttctcc
     7441 tgagaattcc gacagaaaaa gcaaaaataa atg
//
LOCUS       DAJTSI010000175         7182 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000175 DAJTSI010000000
VERSION     DAJTSI010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7182
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.175"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(296..1198)
                     /locus_tag="OO327_003574"
     CDS             complement(296..1198)
                     /locus_tag="OO327_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCR6019970.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTLVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     TDNKQCTLSEARHQLPITPFRMPS"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="OO327_003575"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="OO327_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HCR6019971.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4013
                     /gene="lsrA"
                     /locus_tag="OO327_003576"
                     /pseudo
     CDS             2479..4013
                     /gene="lsrA"
                     /locus_tag="OO327_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
     gene            4007..5035
                     /gene="lsrC"
                     /locus_tag="OO327_003577"
     CDS             4007..5035
                     /gene="lsrC"
                     /locus_tag="OO327_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HCR6019972.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTSGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5035..6027
                     /gene="lsrD"
                     /locus_tag="OO327_003578"
     CDS             5035..6027
                     /gene="lsrD"
                     /locus_tag="OO327_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HCR6019973.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6039..7062
                     /gene="lsrB"
                     /locus_tag="OO327_003579"
                     /pseudo
     CDS             6039..7062
                     /gene="lsrB"
                     /locus_tag="OO327_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            7089..>7182
                     /locus_tag="OO327_003580"
     CDS             7089..>7182
                     /locus_tag="OO327_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HCR6019974.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGA"
BASE COUNT         1677 a         1811 c         1875 g         1819 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cagagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcagcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatacccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgccttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcga aagataatgc caccctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tggaagcttc gccgcaagca ttgattgtcg atgagcgacg cgcggcgtgg
     3781 atgtctcggc ccgtaatgat atctaccagc tgttgcgcag catcgccgca cagaatgtgg
     3841 ctgtgctgtt tatctcctct gatatggaag agatcgaact gatggcagat cgtgtgtatg
     3901 tgatgcatca gggcgaaatt acccactctg cactgaccgg gcgcgatatt aatgtcgaga
     3961 ctattatgcg cgttgccttc ggcgatagtc agcgtcagga ggcatcatgc tgaagtttat
     4021 tcagaacaac cgtgaaatca cggcattgct ggcggtggtg ctgctgtttg tattacccgg
     4081 ttttctcgac cgccagtatt taagtgtgca aacgctgacc atggtttata gcagcgcgca
     4141 aatcctgatc ctgctggcaa tgggcgcgac gctggtaatg cttacgcgca atattgatgt
     4201 ttcagtgggt tcgattaccg gaatgtgcgc ggtgctgttg gggatgttac tgaacgcagg
     4261 atattcacta cctgttgcct gtgtcgcgac tttactgctt ggtttgctcg cgggattttt
     4321 caacggtgcc ctggtcgcgt ggctaaagat ccctgccatt gttgccaccc ttggcacgtt
     4381 agggttgtac agaggcatca tgttgctgtg gactagcggt aaatggattg aagggttacc
     4441 cgccgaactg aaacagctct ccgccccgct gctgcttggc atttcagcaa ttggttggtt
     4501 gacgataatt ctggtggcat ttatggcctg gctgctggcg aagacggcgt ttggacgcag
     4561 tttttatgcc acgggcgata atttacaggg cgctcgtcaa ctgggcgttc gtacggaagc
     4621 cattcgcatt gtggcatttt cgttgaacgg ctgcatggcg gcactggcgg gaattgtgtt
     4681 tgcttcgcag attggtttta tcctcaacca gaccggtacc gggctggaga tgaaagcaat
     4741 tgcagcctgc gtgctgggcg gcattagttt gctcggtggt tccggtgcga tcatcggtgc
     4801 ggtactcggc gcatggttcc tgacgcagat cgatagcgta ctggtgctgt tgcgcattcc
     4861 ggcatggtgg aatgatttta tcgcgggtct ggttctgctg gcggtgctgg tgtttgatgg
     4921 acgcctgcgt tgtgcgctgg aacgtaatct acggcggcaa aaatatgccc gctttatgac
     4981 gccaccgcca tccgttaaac ccgcttcgtc aggtaaaaaa cgggaggccg cataatgcgt
     5041 attcgctacg gttgggaact ggctcttgcc gcactgctcg ttattgagat tgtcgcattt
     5101 ggtgcaatta acccgcgaat gttagatctc aatatgttgc tgttcagcac cagtgacttt
     5161 atctgcattg gcattgtcgc tctaccgctg acgatggtga ttgtcagtgg cgggatcgat
     5221 atttcgtttg gttcgaccat cggcctctgc gccattgcat tgggcgtact gtttcaaagt
     5281 ggtgtgccga tgccgctggc gatactcctg accttactgc tcggcgcatt gtgcgggctg
     5341 atcaacgccg gattaattat ctataccaaa gttaacccgc tggtgattac gcttggcacg
     5401 ctgtatctgt ttgccggaag cgctctgctg ctttccggta tggccggagc gacggggtac
     5461 gaaggtattg gtggattccc gatggcgttt acagatttcg ctaacctgga tgtgctggga
     5521 ctccccgttc cgctgattat cttcctgata tgtctcctcg ttttctggct ctggctgcat
     5581 aaaacccatg ccggacgtaa tgtgtttttg attgggcaaa gcccgcgcgt ggcgctttat
     5641 agcgcgattc cagttaaccg caccttatgt gcgctctatg ccatgacggg gctggcgtct
     5701 gcggtcgccg ctgtgctgct ggtatcgtat tttggttcag cacgttccga tctcggtgcg
     5761 tcgtttctga tgcccgccat caccgccgtg gtgcttggcg gggccaatat ttatggtggt
     5821 tccggttcca ttatcggcac cgccattgcg gttttattag tgggatattt gcagcaaggt
     5881 ttgcaaatgg caggagtgcc aaatcaggtg tccagcgccc tttccggtgc gctgcttatc
     5941 gtcgttgtcg taggtcgttc cgttagcctg catcgccagc aaattaaaga gtggctggcg
     6001 cgtcgggcca ataacccatt gccataaagg atatcttcat gacacttcat cgctttaaga
     6061 aaatcgcctt acttagcgct cttggcattg ccgcaatctc tatgaatgtg caggccgcag
     6121 agcgtattgc atttattccc aaactggttg gcgtgggatt ttttaccagc ggtggcaacg
     6181 gcgcacaaca agcgggtaaa gagctgagcg ttgatgtgac ctacgacggg ccgacagaac
     6241 ccagtgtttc tggtcaggta cagttgatta ataacttcgt caatcaaggt tataacgcca
     6301 ttattcgttt ctgcggtttc gcctgatggc ttgtgtccgg cactgaaacg cgccatgcaa
     6361 cgtggtgtga gagtgctgac ctgggactct gatactaaac cggagtgccg ctcttactac
     6421 attaatcagg gaacgcccgc ccagttggga ggtatgttgg tggatatggc ggcgcgtcag
     6481 gtgaataaag acaaagccaa agtcgcgttt ttctactcaa gccccaccgt tacggaccaa
     6541 aaccagtggg tgaaagaagc gaaagcgaaa atcgccaaag agcatccagg ctgggaaatt
     6601 gtcactacgc agtttggcta taacgatgcc actaaatcgt tacaaaccgc agaaggaata
     6661 ttaaaagcgt atagcgatct cgacgccatt atcgcccccg atgccaacgc cctgcccgct
     6721 gccgcacaag ccgcagaaaa cctgaaaaat gacaaagtag cgattgtcgg attcagtacg
     6781 ccaaatgtga tgcgcccgta tgtagagcgc ggcacggtga aagaatttgg actgtgggat
     6841 gtggttcagc aaggcaaaat ttcagtgtat gtcgcggatg tattattgaa aaaaggatca
     6901 atgaaaacgg gcgacaagct ggatatccag ggcgtaggtc aggttgaagt ctcgccaaac
     6961 agcgttcagg gctatgacta cgaagcggat ggtaatggca tcgtactgtt accggagcgc
     7021 gtgatattca acaaagagaa tatcggcaaa tacgatttct gatgtgcatt acttaaccgg
     7081 agtaagttat ggcagattta gacgatatta aagatggtaa agattttcgt accgatcaac
     7141 cgcaacaaaa tatccctttt accctgaaag gttgcggtgc gc
//
LOCUS       DAJTSI010000176         7091 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000176 DAJTSI010000000
VERSION     DAJTSI010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7091
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.176"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1013)
                     /locus_tag="OO327_003581"
     CDS             complement(<1..1013)
                     /locus_tag="OO327_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HCR6019975.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VIN"
     gene            complement(1381..6043)
                     /locus_tag="OO327_003582"
                     /pseudo
     CDS             complement(1381..6043)
                     /locus_tag="OO327_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            complement(6087..6770)
                     /locus_tag="OO327_003583"
     CDS             complement(6087..6770)
                     /locus_tag="OO327_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR6019976.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
BASE COUNT         1701 a         1735 c         1715 g         1940 t
ORIGIN      
        1 atgttgatca cccactcata cagcgcacca cccgatgcga acagggcttt aaacggcggg
       61 atgctccaca gtgttacggt agcggtcagg aacaggaacg gtgtccaggc acgtaacacc
      121 tgacccgcgg tgtaaccggt gtgggccagc gtcatatcaa cctgtgacgc ccccaagtca
      181 ccgaagcgga atacgcgaac tggttgccag cgtttgagga acagcgtcag gcagagcagt
      241 gataccagcg aagagataat gtccggcagc tccggcccaa tgaagttaga gctaaggtac
      301 tgagcgatgg caaacgagcc gcctgcaacc acgaccgcag gccacgtctc tttgataccg
      361 cgccagccgt ccataatcgc catgatccag aacagcacga taatggtcat aaacggtagc
      421 tgacgcccca ccatctggcc aatctcaaag ctgtcgatgc ctgttacttg tccggcaacc
      481 aggattggaa tgcccatcgc gccaaatgcc actggcgcgg tgttaacaat caggcacagt
      541 ccggcggcgt acagcggttt aaaacccagg ccgaccagca atgcggcggt aattgctacc
      601 ggtgcgccaa agcctgcggc tccttcaagg aaagcgccga aacagaaacc gacgatcagc
      661 atttgcagac gctggtcagg ggttatcgaa agaatagacg agcgaatgat gtcaaactgc
      721 ccggttttca ccgagatctt atagacgaac actgctgcaa taatgatcca cgcgatgggc
      781 cacaaaccat agaagaagcc ataaaccacc gaggccagcg cgttagcgac cggcatttta
      841 tagaacagca aagccacggc aagggcgatt gctaccgtcc atgaggcggc gacgtatcct
      901 ttcagtttga gcttaatcag cgcaaagaag aaaaacagga tgggaagcga tgctatcaga
      961 ctggaaagcc agatattccc ggcgggatcg tagttttgtt gccagagatt cattgcaggt
     1021 ctcctagagt ccacgcacat cgtgtaatga gtctgttgct catctccttg tcacacgttg
     1081 tgtaaaagtg gcactgccaa ttgtgttgtg tagggataat gacaatgaat ggttaactaa
     1141 atgttatgta ttggcaatat tgatgtgagt tttaattggg gaattgtgaa ccagaggtca
     1201 gataagcgaa gaattggtag ggccaattct tgtgggggga gatgtctggt aaagaaaagg
     1261 gcaatcctca tcagagaatt gcccttagat tgttgacccg gagcgcctta tcttgcctga
     1321 tgcgcgtaac gcatcaggca atttggcgtt tgcccttagt cacactaact taatggatca
     1381 ttaccactga ataccggcac cgagtgcggc ggagtattca ccctggctat tggtactacc
     1441 ttgtaatttg tagacccaac gaccattggc gctcaccatc gatacaccta aagcaactgc
     1501 cgattcaccg ttgtaagtac cgccaccaat agaagccatg ctggcacccg gtgtataagc
     1561 ctgcggcaga ccggtcattg ccattgcaga agcgatacca ccgctcaact tgctttcagt
     1621 tttagacagt ttgttatcca tctcaaccat ccgctgatcg gtgtattgct tcgtttcctg
     1681 cgcgctttgc ttcaactgcg cgtagttcac cgcgtcgttg ttgttgacgc cagcggagac
     1741 gttgctgata cgagtcgtac tgccgttgcc accaccgagg gtgatattgc tatagtcgat
     1801 agaaccatca gctttggtgt cgtagcgcac tccgcctgct tcagaagatt tcagctgcga
     1861 cacgttaacc gcatcggtgg cattaacact ggcagctacg ttggtgatac ggcgttggtt
     1921 agtagaagaa cctacagaga ttgtattttc ttcctctgca acggaaccgg tacccagtgc
     1981 gacgctgttg tcagcggcag caatggaacc agaaccaata gcaacgctat ctttaccctg
     2041 ggcgttggca tctacgccat cggtgttggt cttgaagtac ttggtgctac cggtggtgac
     2101 aatgtcgcca ataccgtttt cgatattagt aacgcgagta tccagattag tgacctgatt
     2161 gttcaggtta gtaatgctct gggtattttg cgctacgcgc tcatcagtga ctttcaactg
     2221 acccacgtta accgcatcgg tatccgctga accagctgcg acgtttgtga tctgacgttg
     2281 accgtcttca ctaccgacag aaaactcacc tacagagttc tgcggtgcgt ccatgttgta
     2341 ggcggtgtag ccagtctgtg caccacgggt ggtctgagaa ccgttaccca ttgcgatact
     2401 gtttgcatgg tttgcgcgtg cgttactgcc gatagcaatg ccgttgatcg catcagccag
     2461 aaccagagtg ttcaaaccga taccaatacc tgcattacca ttaacgacag ttttcgcacc
     2521 cattgccagc gagtcttcac caacggccag tgagtcttct gccgttgagt tggcgtgaaa
     2581 gtacttggtt ggcgtagtgg cgaccgctcc aatagcgttc tgcaactggc gaaccgtaac
     2641 ggcgtcatgg gcttctgaac catcagctac gttgatgatt tggcggtatt taccgtcatc
     2701 accaattgac aatgcaccta gcagttcgcc gtcagtggtg tcataaccaa tcaccacgcc
     2761 attttccgtt acgctagtgt cacgagaacc tttggcaatc acacggctgg aaacggagct
     2821 gctacccagc gcaataccgg tatcaacggt gctgctgctg ttctgaccaa tggctacgct
     2881 gctggcacca gacgcgacag cgttataacc tacagctgta gcgccgacac cagaggcgct
     2941 tgcatcgtta aaggctaaat cgttgtcgtt agtacgaacg tagttgatac cagcgccatt
     3001 ttcttcgata taggttgcgt cggtgttacc agcgagctga ttgataattt gggtgttctg
     3061 ctcaatcatc atattcgtcg cattcaactg agaaccgttc actgcatcgg tactgtcctc
     3121 actaatactg ccattagcga cattagtgat gatgctggct ttaccgtcat ggctggcatt
     3181 gtaggctcca gcaccgccat tggcagtctt atcccacagc agggcgttat catcaagcgc
     3241 atccagggcg tcaccgacgt tgttgtactc ggcgttcgca atggtgtaag tcggtgcggt
     3301 gatggtgccg tcagcgttga cttctgcgtc accacccagc gcatcagcga tgtacttatt
     3361 ggtggtatag agttgtgagc cgttaatcgc gtcgctgctg gcagcagaga ttgcaccgtt
     3421 agcgacgtta gtgattacac tggcagtttt atcttttccg tgagcggcgc taaatgcacc
     3481 attttcaccg gcgtccgcat cccagagcag agcgtcgtca ggagtggtat cgatagcatt
     3541 cagggcatca ccgacattat cgtaatcagc attagcaatg gtgtacgtcg gcgcagtgat
     3601 ggtgccgtct gcattgactt cggcaccacc ccccagcgca tcaacaacat aactgctcac
     3661 gccgtggagt tgtgagccgt ttactgcgtc agaactggag tctgaaattt caccatctgc
     3721 gacatcagtg atcacgcttg cgtcaccatt agtaccgtgt ttggcactga atttacctgc
     3781 ggtggcatcc caaagcagag catcgccgag agacgtgcta aatgaggagt taatcgcagc
     3841 cagtgcatcg ccgacgttat aataatttgt ttcaccgatt ttgtaggttg gaccggtaaa
     3901 cacaccgtca gtattcacag aagcattgcc gccgaagtag gtggcgatat tgctgcttaa
     3961 gtcataaagc tggctaccgt taatggcatc ggaactggtt gcagtgactg tgccgtccag
     4021 gatattggtg attttgctgg cggtattgtt gccatgagct gcagtgaaga caccattgtc
     4081 cttatcccat ttcagcgcat cctcaccaag attagtcacc gtctcagtca gattagagat
     4141 gttggtggtg ttagtggcga tattggtggt attcgtcgcc acggcatcgt tggtggtttt
     4201 cagctgagag ccgttaacgg catcggtgct gccagtcgtc aggtcgccat ctttaacgtt
     4261 ggttattttg ctggtggtct cggtgccatg agctgccgtg aatacaccat tgtccttatc
     4321 ccatttcagc gcatcctcac caagattagt caccgtctca gtcaggttag agatgttggt
     4381 ggtgttagtg gcaatattgg aagtgttatt ggcgatattg gtggtattcg tcgccacagc
     4441 atcgttggtg gttttcagct gagagccgtt aacagcatca gtgctgtcag ccgtcaggtc
     4501 ggcatcttta acgttggtga ttttgctggt ggtatcctgg ccgtgagctg cactgaatgc
     4561 ctttttagtt tcgttccaga gcagggcatc agcctgaagg tcaccaacgg aatccgtcag
     4621 gttggtgata ttggtggtgt tggcttcgac atcgtcattg gtagctttca gttgggaacc
     4681 gttaaccgca tccttactgg aggctgaaat cgtgccatcc gcaacatcgg tgattacgct
     4741 ggcagttggg ctactagtac catgagcagc gctgtatttg cctttggttt ggtcccattg
     4801 cagggtgttt tcatcaagta cagcgagcgc agcccctacg ttatttttgc taccattttt
     4861 taaattgtag gttggtgctg taacagtacc gtcatcaaca tctactgcag cccctcctcc
     4921 aagccttttt tgctaccgag tcgctaatgg cataaagctg tgaaccatta atggcatcgt
     4981 aactgtcaga cttgatcgcg ccatttttca cattaacgat cttgcgtttt aatgaactat
     5041 tgccgacaga gacggtattg tctaaatccg caatggaatc tgcgcccagc gcgacagagt
     5101 taataccgtt ggcactgcta ttactaccaa gcgcaagact cttatcacca gttgcattac
     5161 tggctgtgcc taaggcaatg gtgtttaagc ctctcgactg gctaccatta cccagagcga
     5221 tggcatccac gccatcagct ttgttgccct ggcctatggc gattgcatta tctgccagag
     5281 cctggctgcc atttcctaat gcgatagtat tgactttatc ggcaaggctt ccgaacccca
     5341 ttgccatgct attagtccct tttgccttcg cattattgcc aatagcagtt gcattttcag
     5401 cggcttcggt ctcagcacct atcgcaatag cgttcgctgc gctggcaagt gattgtctgc
     5461 caaatgcgag gctgtcagta ccgctagcta cactgctacg ccccagggca atggcgtttg
     5521 ctccttccgc tttggaagag tcccccatag caatagacaa tttacccaat gcttttgaat
     5581 aacggccgag ggccattgag tattggccca atgaatacga agaggcaccc agtgctatac
     5641 ttctatcgcc ttctgatatt gcactaaccc caaaggccat tgatgcacca ccaatcgcat
     5701 gggtttttga cccgatggca gagctatatt ggccttcagc tatagcgtca taacccacag
     5761 cagtactgga accactggtg ttcataaaag tatttgcttt cgcccctttg cctatagcaa
     5821 cccagccgtc gccagctgat ccactaccgt aatcaacacc ctgaccgttg tcattcccgg
     5881 cattcgccaa tgccccaaac gacgacaaca ttccacccgc aaccagcgca gaaattaaca
     5941 gcttactgcg cccagatttc ttgccacggc tttttgccgt ttcgctggta acagtataat
     6001 tccctgtcgc agggttccag ataactttaa atattttgtt cattaatgat gctcgctatt
     6061 ccttgttata agcgtactaa ctgcatttat tttttgcttc ttttctgccg attttcagca
     6121 gagggaagat tgtcgaactg aatattttcc atgttgatgg ctaacttcag atatcccagg
     6181 ttattgggcg atacaccctt cagggtcaaa ttaatgtctt gccattgacc tggtaataat
     6241 ttcaatgtgg atttcattga tgtgttgaaa agtaattgtt gccaccgggc aaactctttg
     6301 gcatcgacag gtttttcacc accccatgtc gcattaaaac caatgtttga gataggtgta
     6361 cttaataagt tgaccagtga gatatgaata acactaccat tctctgtggc gtcaatggat
     6421 aacactcgac ccgctaaagc caccgaaccg ttgttcagga tgaaatactt cgtttttgct
     6481 gggttaaact cggtaaaaat aaaagtattt tgttggtctt taacggattt aattacgtga
     6541 gttaaagtct ttgtactgtt tattatgcca gtttgttgtt tttgaatagt ttcgagttga
     6601 ctctttagct gtgcttgttg tttttcaagt agcacaattt tattattatg ttcttcttgt
     6661 tgtttcagta aactactttc tggcttgcct tcttcaatgt agtcacatcc cgttaatgcg
     6721 cagagaactg aaacagtgac agcactgaaa aaaatcttct tcaagttcat gtaaaaatcc
     6781 ttatttcctc cgaccagcgc tcaatgccgg tagagttgtc cattgtatta ttggtgagct
     6841 atgttgcttt acattaatga cagtgatgtg tgtcgattaa atattgtttt aagcattcat
     6901 cataaaaatg tatcattcgt ttcaaaagat gtctgtgttt gtatgaatat attatttttc
     6961 gtggattcta atgatgatta ttaataaatg tcaaatatga aagaaaccat gtctgggaaa
     7021 aatttcacag ataattgatt ttgtgaatat gatgattaaa atttcacaat taaatccgaa
     7081 aaaaagagac g
//
LOCUS       DAJTSI010000177         6962 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000177 DAJTSI010000000
VERSION     DAJTSI010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6962
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.177"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(365..946)
                     /locus_tag="OO327_003584"
     CDS             complement(365..946)
                     /locus_tag="OO327_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019977.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(993..4850)
                     /gene="sigA"
                     /locus_tag="OO327_003585"
     CDS             complement(993..4850)
                     /gene="sigA"
                     /locus_tag="OO327_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCR6019978.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGVLTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(5211..6215)
                     /locus_tag="OO327_003586"
                     /pseudo
     CDS             complement(5211..6215)
                     /locus_tag="OO327_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            6200..6577
                     /locus_tag="OO327_003587"
                     /pseudo
     CDS             6200..6577
                     /locus_tag="OO327_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6616..>6962)
                     /locus_tag="OO327_003588"
     CDS             complement(6616..>6962)
                     /locus_tag="OO327_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6019979.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT         1734 a         1568 c         1572 g         2088 t
ORIGIN      
        1 catcacctgc tattaatccc cgagtcagta cttcgcggcc tgacgcgatc accctactca
       61 gtacttcaga gcccgcctcc ggtacctgcc tggcgatcgc ccctgtgcaa caaaataaat
      121 caaccgttat caaaccacga tcgttcgtat gcgccgccga tgacgcgcgc ctgaaaaacg
      181 cgtcaatatg agttccccgg aagcttacga taatacgctt atgaacatta caacacgctc
      241 atgcggaacc tggatggggg aaggaacggt gaagctgtta gcgaatgacc ggcgtttata
      301 caaacgccgg tcatgatgaa tgatatttca gaatatacct cgggctaaaa taccgggaca
      361 gagatcaggc gtgattaatc tgcagcattt ttaataacag aagtactgag cttactttac
      421 ctttgatgat gtaatagtta tttccgtcgg tactcatttt tccctgaaat aatgtcacgg
      481 atgtttcttc ctgctgatcc tgcaggcgca gtcgtaaggt catattttta atgagctgag
      541 atccgggtgg caaggggtat ttgctgtcag atactgggcg actgagtctg atgtggcgcg
      601 tatacagccc gttttcgtac agggtggcct ctgttatggt gtcagtgacc agcatgtggg
      661 agacaatgct gtattctttt ttaaagcacc caccagaaac ggtgattgta atgataatgg
      721 tcttctcgat cgagatgtaa atacccgttt cgttatactg tccgtatcgg aaccatgaga
      781 actcagttgc attccagcca tgctccagaa tatgtgcatt tttttgcctg atagtttctt
      841 tttttattcg ctcatgttta aggcataaca ggcagataaa taatgtcaga aaaagaaagc
      901 taataatgag agcaagagcg atatagtctg tcacagggta atacatattc tctccggtag
      961 aagaagggcc gcaaacgcgg cccgggctgt tatcagaaag agtaacggaa gttggcgttg
     1021 atcgcgttat ccacgttgta tttaccaaat gccgatttct caaactcaag accgaagcga
     1081 agattatcgc gaatttcggc gttgagacca acattcatga gaatacgacc gtctttttca
     1141 cctttgatac gtttctcacc ggacgcatca cgcagtacgg tttcaccgtt ggcaaacagg
     1201 tcaaactggt agccaaggcc ggcgcgggct gtgactttcc agtccttacc ggagaaggat
     1261 ttacccacat caacaccggt acgcccaatc agcggattaa agtccttatc cttcatggtc
     1321 acgctcattc cgcgatcctg ccagtcgaac cgtttacctg atacggcccc ataaaccagt
     1381 tctgcctgcg gctcaatcca ggagtcttct gttacatgat agcggtagcc tgcttcagca
     1441 cccacataca aggaatgaga actgtagtct tttgttccga gtccagcaaa ggtcgccgag
     1501 tactcattat catggtgaac atacttacta atcaggtcga tataggcacc agagtcaaat
     1561 atagcggaag catacagacc tgccccgacg gacttcgtct tgccactgaa tgcattactg
     1621 ctggcatgac tgtcggtata cgtcatagtc agaccagtga aaaggtcaag tccatccagc
     1681 tcatgttttt tatccacacc aatctgcaca tgggtgtagt tgtcactgta tccaccagaa
     1741 gctgaacctg ctccgctcat gatgcgggcc catgcaccgg cctcgccatt ggtgtcacgc
     1801 agatccccca ttcgtttatt caggttgttg acttctgtca ggaaggcttt gtaattaccg
     1861 gacatatacg attttgctgc accggaagat gctgtatttt cagccacctt atatccggtc
     1921 agggtccacg tggtatgttc ctgatcctct ttcgtgctga tcacaggaga tatgttgctg
     1981 aaacctatgg cctgagtact ggctttaaat atctctttat tgcttccttt tggggctgtt
     2041 atcaggtcaa cgtttaatcc tgagttatta tttcccactt tctgtatatc gaccgacaga
     2101 ctgttattgc ttccggttaa ctgattttta acaatgagtt tatccgattg atttacagac
     2161 tggctcagat cagcccgcat aatcacagca gtattagtgg catccagtgt gtttactgtc
     2221 acagtatgga aacccgaatt ctctatgtta ctaaacaaca aaagactgtc gcgggttttt
     2281 aatgattgca gggctgactg tccgtttact ctccataagg tgttatacat gctggctgag
     2341 gctgcaggag catcaatacc tccggtataa gaagtatcaa agccacccag caaacctgta
     2401 gccagcggtg tgtgtgtggt tttgtccagg ttatcttctt tatactggtc aacaccaaac
     2461 agaatagatg cttttttgtc tgattttata ttaccattga cccaggcata attttgagcc
     2521 tttaacgtgg caccatcttc tgtcatatta tatccatctg acatatagat tgccgggctg
     2581 tatttattat tttctgtagg ggtgcctgtt aatgtcaggg ttccggccag ttcaatagct
     2641 ttatctacat acatttttcc ctgggcggta acagtagctc ctttgttgat aatcaggtct
     2701 gtattaatta aggataatgc accagtcata gtaacagttg atttatcacc aacagttatt
     2761 ttgctgtttt gagcactcag actgccgttg aaactacttt cgcctgctga atccccggag
     2821 cgaagctgtt gtctgaatcc aaaaccttcg ccagtaatgt ttcttcctga gaacatgtca
     2881 atgtatgcag ttttatttcc cagaacaatt tgagagtttt tagcctctat gtgtcctttt
     2941 acattagcat cccggcctat actcagggtt gcgttttcta aatttaattt ccggaaatta
     3001 aatttacgac tttcccagtc gggctgggaa aaagaggcta tctgattgtt ggacttatat
     3061 tccgtattgt aacgtttatt tacagtgctt tcctggtcct ttatggcagc agaatagtct
     3121 ttttgacata aaaaaggaat tggacagttg ttattgcctt ctttaaatat ggcatgttct
     3181 gtcgcatgtc cttgtaatgt taactgtgca ttccggacag agatatcgtt tttgatatcg
     3241 actgagccat caaagatcag attggcatta ttgttatgtt gctgggtagt attgataata
     3301 tgatttatgc ttatattacc tgatacattc ccatggtaca tatatgcatc agtattagta
     3361 agtgataatg ttgattgctt cacgtttgag ttcgttacgg ttgttcccgc atctgttgct
     3421 gcgattttct gaaatgactg gtcatagccg tttagatcca gtgttccgcc atttctggta
     3481 aaatagattt cgccattacc gctttcgctt aaagcgtctt tggcatttat ttttaccgta
     3541 cctttacctc cggccatgta aattttattg aaggcttttt cagcactaag gatgacagtc
     3601 ccattaccta ttttaaggtt atttccctgt gctattttta catccagagt accactacct
     3661 attttatgca gattatctcc ggcaacgccc ttaacattcc attcaacggt ggtattatta
     3721 tcaatatcta tcccggcacc tttataagac ttatcttttc cgtacacttt atatgtctgg
     3781 ttcttatcga atataagccc gccaatgcca agatcggtat cctgcttgag cgttaatacc
     3841 cctcctcctg agacagacag gtccttatct tttataatcg atgataactc ttgtttttta
     3901 ccatccagct gcatagtgtt tgtgaaattc ttttttaaat tgtttaccag aatattatta
     3961 aataacgagt agtttgtcca ggctatattg gtactactga taaaaccaac tgtactggta
     4021 acaccatgga aaacccattt ttgttgttct ttatcatata gataagaacc agaaccacta
     4081 tcgccaaaaa ttggtgagtt tttaaaaggg gttctgttgg tataaatgta actcttgttt
     4141 ttccagtcca gattaaaaag acttgcgctt aacaagtcgg gattatagtt ttgtccaaac
     4201 agatattgtt tgccgtttaa tatcgttgag gttgttccgg cacctgctct gaagccaatt
     4261 atttgttcct taccgttgta gtttacgcca tatcttttca tcgcatcttc gggagaaagt
     4321 gagaagtcgg cactctccgt aacccctgtt gtttccacga cgaatttatt cagacgatga
     4381 accgaaaagt cgccagatga tactctgtta cttgctttat atgctgtctg atcccatgac
     4441 tgtgttgtta ttgcatgatg ctgtgtaccg ttatgagtcg cagtaacact gtacgcacct
     4501 ccaattgatg ttgttgcacc tttgttggaa acagcagaaa aatcaggtac aggtagttct
     4561 ggaaaatgaa atatctttcc atctttcatc attaattgaa catttgttgc acccggtctg
     4621 aactctcctc tgttttgagc aaggtcaaga taatcgcggg tccatatatt atttatatcc
     4681 agcattgctg caaatgctgt ttcgccataa cttccatata ttgaggataa aataataccg
     4741 aggataactt tctttcttga tgtaccgaca ctaactttcc gggtcagttc agaaacagca
     4801 actaatccac ctgtaatatg actatatttc agtgaataaa ttttattcat acgtgaactc
     4861 cgatatttct gagttgtggt tatgggatag catttaatta caacggggaa ctatcagtca
     4921 tggtacgtaa ctgataaggg tataatgata atcactgtca gttatatttg tatgacagat
     4981 gtaacaaaat gaaaaaaaag ataagcacaa gtgattgatt ggttagatat tgatcacttg
     5041 gtgtttgtat tttgaatttt gcgatcaaaa tttgatcttt gttgtttccg gtcaatcctt
     5101 tacaggtttt ctgtattatc cgggggagtt aagtctgtcg atgtccgaca gacttttgat
     5161 gatggaaaca acatgcagcg ataatgtgca tgctgttttg caggtggaac tcagaaggtg
     5221 aactggaact gtgcgaccgc accgtaagtg ttgtccgtgc cgatcaggcc tgcaacatca
     5281 aggtgaacca catcaaacgg tgagatcccc accccggccg tgaatgcact gccgtcgtta
     5341 ccggccagat tctgacggta accggcgcgc agctgtgccc atttccaggc attaaattct
     5401 gtgccaatgg cggcaaactg acggttgctg tcggaggtga aaccgctggc cggggtcaga
     5461 tccacatcaa atgcagtggt gaacatcgcg ttgtgccagg agacaccggc cgtcacctgc
     5521 gggcggatcc tgaaggtttc cgtgataccg ttcacttctt ttgtctcaat actacgggag
     5581 atcaggttct gtgcgaccag tcccagcgtc cagttgtcat ccaggtccgt actggcaccg
     5641 atgtcggcgt tgatgccatt tttcgtgttg tggtagcggt cgcctttgaa ggcgctgctg
     5701 tcataatttc tgaccagcac gttgtagttg aacaggtcga cgcgctggaa tttggggggg
     5761 aagcccagag accatttctg cccggccgtt tccagttccg tagccagagc aatacccaca
     5821 tcagtgatca gtgccgtgcg ggcacaggcc tgtgatgtca ggcggctttt gtccacctcc
     5881 tgcccggtgc tgtttgccac ctcttccagg taagtgaggt cagcatcaga gactttaccg
     5941 tcgacactga cggtgccgtg tgatttaacc atcagtgcgg cagagaggcg atcaccggga
     6001 agggccgcca cagcagaaac gcccagctgt gcggcggcat gtgtgttacg gaagtcccgg
     6061 aggtgttctt tcaggcgtgc ggcagcctca ggaacaccgt ggttactttc gatggcgcgg
     6121 tcgaaggcct tccactcatc actgatccgg tcggcgttgt cggtgatatt atccggattg
     6181 gagagctgcg cgccgacgga cggaagcagg atgctgggct catgcgcgac gcaaaatcca
     6241 cgatgtatat atcagtacta aaagcgcgac ggcggaagaa gctctgaaac taatcggtga
     6301 actgtacgcc atagagcacg atatacgcgg gttaccggtg tctgaacgcc tggcggtcag
     6361 gcaaatgcag agtagaccgc tcctgacttc cctgtataag ctgatgcagg agaaagaaca
     6421 cacgttatcg aagaaatgtc gtctgggaga tgcgttccgg tatatcagga agcactgggc
     6481 tgcgttgtgc aacttctgtg atgatggcct ggctgaggta gataataatg cagcagaaag
     6541 agcgcttcgt gcagtctgtc tcgggaagaa aactttttcg aggatcactc attccgtgtc
     6601 tgactgcttc agggtttaat tttgcttctg ctgactgaat gatgattggg cagcaagtgc
     6661 aatatcccac ataaatccag tcaactcacg agccaccgcc gtaatggcta cttgtgattt
     6721 tttccctttt gctgtcagag ttcggtaacg agaacacaat cgttgttgag ctttccatgc
     6781 aatatctttg gaatattgtg ttacagagtc cggtcggtaa tatataagcc atgctccaac
     6841 ttttgcaggt gtacgataag accaggcggc ttcgtaaagc agacgtctca gttcgctgtt
     6901 tccaactttt gttattcctc tgggacgaat actgtttcca ctggaatatt cacctggcac
     6961 ca
//
LOCUS       DAJTSI010000178         6958 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000178 DAJTSI010000000
VERSION     DAJTSI010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6958
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.178"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..995
                     /gene="galM"
                     /locus_tag="OO327_003589"
     CDS             <1..995
                     /gene="galM"
                     /locus_tag="OO327_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR6019980.1"
                     /translation="LLTLRNNAGMVVTLMDWGATLLSARIPLSDGSVREALLGCASPE
                     CYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQLHGGPEGFDKRRWQIV
                     NQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITYRATVDKPCPVNMTNHV
                     YFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGTSFDFRSAKIIASEFLA
                     DDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYTTAPALQFYSGNFLGGT
                     PSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEEYSSLTEYQFIAE"
     gene            1198..1950
                     /gene="gpmA"
                     /locus_tag="OO327_003590"
     CDS             1198..1950
                     /gene="gpmA"
                     /locus_tag="OO327_003590"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR6019981.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(2117..3169)
                     /gene="aroG"
                     /locus_tag="OO327_003591"
     CDS             complement(2117..3169)
                     /gene="aroG"
                     /locus_tag="OO327_003591"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR6019982.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            3485..3865
                     /locus_tag="OO327_003592"
     CDS             3485..3865
                     /locus_tag="OO327_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR6019983.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            3979..4920
                     /gene="zitB"
                     /locus_tag="OO327_003593"
     CDS             3979..4920
                     /gene="zitB"
                     /locus_tag="OO327_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR6019984.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(4917..5636)
                     /gene="pnuC"
                     /locus_tag="OO327_003594"
     CDS             complement(4917..5636)
                     /gene="pnuC"
                     /locus_tag="OO327_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR6019985.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(5674..6717)
                     /gene="nadA"
                     /locus_tag="OO327_003595"
     CDS             complement(5674..6717)
                     /gene="nadA"
                     /locus_tag="OO327_003595"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR6019986.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
BASE COUNT         1763 a         1781 c         1698 g         1716 t
ORIGIN      
        1 gactgttaac tttgcgtaac aacgcaggga tggtagtcac gctgatggac tggggtgcga
       61 ctttactttc cgcccgtatt ccgctttccg atggcagcgt ccgcgaggcg ctgctcggct
      121 gtgccagtcc ggaatgctat caggatcagg ctgcgtttct gggggcctct attggtcgtt
      181 atgccaaccg catcgccaat agtcgctata cctttgacgg tgaaaccgtg acgctttcgc
      241 caagccaggg cgttaaccag ctgcacggcg gcccggaagg gttcgacaaa cgtcgctggc
      301 agattgtgaa ccagaacgat cgtcaggtgc tgtttgccct gagttcagat gatggcgatc
      361 agggcttccc gggtaatctc ggcgcgacgg tgcaatatcg tctgaccgac gataaccgta
      421 tctccattac ttatcgcgcc acagttgata aaccttgccc ggtgaatatg actaatcacg
      481 tctatttcaa tcttgacggc gagcagtctg acgtgcgcaa tcacaagttg cagattctgg
      541 cggacgaata tcttccggtt gatgaaggcg gcattccgca cgacggcttg aaatctgtcg
      601 ccggaacgtc ttttgatttc cgcagcgcca aaatcatcgc cagtgagttt cttgccgacg
      661 acgatcagcg caaagtgaaa ggttacgatc acgcattctt gttacaggcc aaaggcgatg
      721 gcaagaaagt ggcggcgcat gtctggtcag cagatgaaaa attgcagctg aaggtctaca
      781 ccaccgctcc ggctcttcaa ttctactccg gcaacttcct cggcggcaca ccgtcgcggg
      841 gaaccgaacc ttacgccgac tggcaagggc tggcgttgga aagcgagttt ctgccggaca
      901 gcccgaacca ccctgaatgg ccgcaaccgg actgcttcct gcgtcctggc gaagagtatt
      961 ccagcctgac ggaatatcag tttattgctg agtaatgtta agccctcgtc atgagggctt
     1021 tatcttcata ttgttcaaat caccagcaaa caccgacata tttgcaactc aatattcaca
     1081 acaaccttac actgcgccac tattttcgct atggttatgc gtaagcattg ctgttgcttc
     1141 gtcacggcaa tataatgaga attattatca ttaaaagatg atttgaggag taagcatatg
     1201 gctgtaacta agctggttct ggttcgtcat ggcgaaagtc agtggaacaa agaaaaccgt
     1261 ttcaccggtt ggtacgacgt ggatctgtct gagaaaggcg taagcgaagc aaaagcagca
     1321 ggtaagctgc tgaaagagga aggttacagc tttgactttg cttacacttc tgtgctgaaa
     1381 cgcgctatcc ataccctgtg gaatgtgctg gacgaactgg atcaggcatg gctgcccgtt
     1441 gagaaatcct ggaaactgaa cgaacgtcac tacggtgcgt tgcagggtct gaacaaagcg
     1501 gaaactgctg aaaagtatgg cgacgagcag gtgaaacagt ggcgtcgtgg ttttgcagtg
     1561 actccgccgg aactgactaa agatgatgag cgttatccgg gtcacgatcc gcgttacgcg
     1621 aaactgagcg agaaagaatt gccgctgacg gaaagcctgg cgctgaccat tgaccgcgtg
     1681 atcccttact ggaatgaaac cattctgccg cgtatgaaga gcggtgagcg cgtaatcatc
     1741 gccgcacacg gtaactccct gcgtgcgctg gtgaaatatc ttgataacat gagcgaagaa
     1801 gagattcttg agctgaatat cccgactggc gtgccgctgg tgtatgagtt cgacgagaat
     1861 ttcaaaccgc tgaaacgcta ttatctgggt aatgctgacg agatcgcggc gaaagcagca
     1921 gccgttgcga accagggtaa agcgaagtaa acgtcattcg tttaaaaaat gagaaagccg
     1981 actgcaagtg agtcggcttt tttgtttgtc gttgctaaca atggagcaca ttgcctgatg
     2041 cgacgctgcg cgtcttatca ggcctgtggt gattcatcgg atacgccact ctgacggcgc
     2101 atccgacaat taaaccttac ccgcgacgcg cttttactgc attcaccagt tgacgtaaca
     2161 gagcatcggt atcttcccag ccaatgcagg catcggtgat gctcttaccg taggccagcg
     2221 gctccccgct atcgaggctc tgattgcctt ccaccagatg gctttccacc atcacgccaa
     2281 taatggcctt ttcgccaccg gcaatctgct ggcaaacgtc agcacaaaca tccatctgct
     2341 ttttgaattg tttggacgaa ttagcatggc tgaaatcgat catcacctga gctggcagac
     2401 ctgctttgtt cagcccttct ttcacttcag caacgtgctt cgcgctgtag ttaggctctt
     2461 taccgccgcg cagaatgata tggcaatcgc cgttaccact ggtattcaca atcgccgaat
     2521 gcccccattt agttacggac aggaagcagt gcggcgcacc ggcggcatta atggcatcga
     2581 tagccacttt aatcgtaccg tctgtgccat ttttaaaacc taccggacaa gaaagaccag
     2641 acgccagttc gcggtgcacc tgcgattccg tggtacgtgc accaattgcg ccccagctca
     2701 tcaggtcagc gagatactga ggagtgatca tatcgagaaa ctcacccgcc gctggcagac
     2761 cgctgtcgtt aatatcaagc agcaatttac gggctatacg cagaccgtcg ttgatctgga
     2821 agctgttatc catatgcgga tcgttaatca accctttcca gcccaccgtg gtacgcggct
     2881 tttcaaaata gacgcgcatt acgatttcca gctcatcttt cagctcttca cgcagcgcca
     2941 gcaagcgagt ggcatactct ttagccgcga caggatcatg aattgagcac gggccaatca
     3001 ccaccaacag gcgatcatca ttacctttca ggatcttatg gatcgctttt cgggcatggg
     3061 caaccgtatt cgcggcattt tcagttgcgg ggaatttttc cagcaatgcg acaggaggaa
     3121 gtaactcttt gatttctttg atgcgtaaat cgtcgttctg ataattcatg tctgttccag
     3181 tgttgccata cttatcttag tgaatgcaat acttccaatc tatatcttcc gtcagaatgt
     3241 gtaaacgggg ttttacacta tgaacggatt aatcctggaa ttagcaagaa aaacgccaga
     3301 ttgtcgcgaa aaacgagatc tctcctacaa tttctaactg taactccttt gtttgttaat
     3361 tatttcaaga ttctctgctg cgtttcataa actggctgaa atcttaaacc aatgccttat
     3421 attcacctgc aaatgcactg ttggaagagg ttatccgaca taacgaccat aacaggagca
     3481 tcctatgaaa atgacaaaac tggccacatt atttctgact gccactctaa gccttgccag
     3541 cggtgccgca ctggctgccg atagcggagc gcaaactaat aacggccagg caaacgccgc
     3601 agctgatgcg ggccaggtag cccctgacgc ccgtgaaaat gtcgcgccaa ataacgtcga
     3661 caataacggg gtaaataccg gttctggcgg cacaatgctg cattcggatg gttcttcaat
     3721 gaacaatgac ggaatgacca aagatgaaga gcacaagaac accatgtgca aagatggtcg
     3781 atgtccggat atcaataaaa aggtacaaac cggtgatggc atcaataatg atgtcgatac
     3841 caaaaccgac ggtaccacgc agtaaccctg caaaatagcc aataatggga gagcggagtc
     3901 tctccctttt aatttttcac ttctaaaagc agtatgatgt cataaagtta agtgagaata
     3961 attaagggat gactcgtcat ggcgcactca cactcacaca cgtcctcaca cctgccagaa
     4021 gataataatg ctcgtcgctt gttgtatgct ttcggcgtga ctgccgggtt tatgctggta
     4081 gaagtcgttg gtggttttct ttctggttct ctggcattgc tggccgatgc gggtcatatg
     4141 ttgaccgata ctgccgccct gcttttcgcc ctactcgccg tgcaattttc acgtcgtcct
     4201 cccaccattc gccacacttt cggttggcta agactgacca cgctcgcggc gtttgtgaat
     4261 gccatcgccc tcgtggtaat taccattttg attgtctggg aggcgataga acgcttccgc
     4321 acgccgcgtc cggtcgaggg aggcatgatg atggcaattg ccgtggctgg gttgctggca
     4381 aatatacttt ctttctggtt acttcatcac ggcagcgaag agaaaaacct caacgtacga
     4441 gcagcggcac tgcatgtgct cggggacctg ctgggttcgg ttggggctat tattgccgcg
     4501 ttgattatta tctggactgg ctggacacct gctgacccca ttctctcgat actggtgtcg
     4561 cttctggttc tgcgtagcgc gtggcgactg ttgaaagata gcgtgaatga attacttgaa
     4621 ggtgcaccgg tatcgctgga tatcgctgaa ctgaagcgtc gtatgtgccg ggaaatcccg
     4681 gaagtccgca atgtgcacca tgtacatgta tggatggtag gcgagaagcc ggtgatgacg
     4741 ttgcatgtgc aggtgatccc gccacacgat cacgatgcct tgttggatca gatccaacac
     4801 tacctgatgg atcactatca gattgagcac accaccattc agatggaata tcaaccttgt
     4861 cataggccag actgccacct gaatgaaggg gtgtctggcc attcacatca tcaccattaa
     4921 tgggacagcg cgcgtgagcc tctttcacgt gcgctgttga tccacatccg gctgccgttg
     4981 agcgcaataa aggtcaggat gatgtactcc agagacatgg cgtaaacgcc ctgaagtgca
     5041 aaaataacga cgctaatcac gttaataatc acccacaaca gccagttttc cacatactta
     5101 cgcgtcatca gaatcattgc cacgatagat aacaccatca tgcatgaatc ccagaacggg
     5161 aaagcgtccg gttgcagttc aggcatcaca acctgtaatc ctaatgcttg catgatcatg
     5221 actgccacgc gggtcaaaaa tgcaaacacc ggattgataa ataccgtcat cagaccaatc
     5281 gaaacaacgc aaaccgccaa ccagctgagt gccttcggca atggcaacca gcgaattttc
     5341 aactccgcct cgttctgact ggtttgtcgc gaccacgcat accagccgta aatattcgcg
     5401 gcaaagaaaa acacctgtaa tagcaggctg gcatacagct gaatctgaaa gaaaataatg
     5461 ccaaacaagg taacgttgat caggccaaag aagtagttgc tgatcttctc cagactggca
     5521 aggccaatac acagcaaccc ggcgatcgtg cctaccgctt cgatccatga gagatcataa
     5581 ccgcccgccc ctattggtat atgtaccagg atattctgca cactaaaaaa atccatcttt
     5641 cccccaaagc gtaacgttac gccttattat tcgttatcca cgtagtgtag ccgcaaaatc
     5701 cagcatacga ttgagcggca ccagcgccct ctctcgcagc ctctcatcga catgaacctc
     5761 gtgattactt ccttcctgtt ctaatgcctc tgcgatagcc tgaaggtcat tcatggccat
     5821 ccacggacaa tgcgcgcagc tgcggcaggt tgcgccctca cccgcggttg gtgcttccag
     5881 taactcttta tctggcaccg cctgctgcat tttgtagaaa ataccccgat cggttgccac
     5941 aataagcctc tgatgaggca atgcttttgc agcagcgatc agttgactgg tggaaccgac
     6001 cgcatccgcc atatcgacaa tagcttgtgg tgattctgga tgcaccagta tggcagcatc
     6061 cgggtattct tcttgcaagc gggttaacgc ctgagtctta aattcatcat gcacaatgca
     6121 ggcaccctgc cagcataaaa tgtctccacc cgtctgtttt tgcacgtaac gccccagatg
     6181 tttgtcgggt gcccagatga ttttttcacc caaactatca agatgatcaa taagttcgac
     6241 ggcaatgctt gaagttacca cccaatctgc gcgcgctttt accgcagcag aagtgttggc
     6301 gtagacgacg acagtacgat cgggatgggc atcgtaaaat gcgttaaatt cttcaacagg
     6361 gcagccgaga tccagtgaac actcaacctg aagtgtcggc atcagaattg ttttttccgg
     6421 actgagaatt ttggcggttt ctcccataaa tctcacccca gcgactaaca aagtagaagc
     6481 gggatgcttt gcaccgaagc gcgccatttc cagagaatca gaaatacagc caccggtttc
     6541 ttctgccagt tgttgaattt cgggatcggt atagtagtgg gcaaccatca ccgcattacg
     6601 ttcttttagc agacgtttta tcttctcgcg gtaatacgct ttttcatcaa tgcttaacgg
     6661 cgtcggcttc ggggggaaag gataaatcgc cgtgtctgga tcaaacatta cgctcatctt
     6721 accatctcgt tttacaggct taacgttaaa accgacatta gcgtaacatt cgctgttatc
     6781 ggtgtaattt gttttatatg ctaaacaaga tagtcgaatg gtggggaaaa gtcactcgaa
     6841 ttacgcctaa ttccaccgac gtagagttgc ttgcacagta agctctgacg atgtcatcag
     6901 gtttacaaag tgaaattgag gatgcctgaa tagcaaaaaa gcgcctttag ggcgcttt
//
LOCUS       DAJTSI010000179         6921 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000179 DAJTSI010000000
VERSION     DAJTSI010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6921
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.179"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            73..936
                     /gene="pdxY"
                     /locus_tag="OO327_003596"
     CDS             73..936
                     /gene="pdxY"
                     /locus_tag="OO327_003596"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR6019987.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(980..1585)
                     /gene="gstA"
                     /locus_tag="OO327_003597"
     CDS             complement(980..1585)
                     /gene="gstA"
                     /locus_tag="OO327_003597"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR6019988.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(1691..3193)
                     /gene="dtpA"
                     /locus_tag="OO327_003598"
     CDS             complement(1691..3193)
                     /gene="dtpA"
                     /locus_tag="OO327_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR6019989.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(3804..4439)
                     /gene="nth"
                     /locus_tag="OO327_003599"
     CDS             complement(3804..4439)
                     /gene="nth"
                     /locus_tag="OO327_003599"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR6019990.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(4439..5134)
                     /gene="rsxE"
                     /locus_tag="OO327_003600"
     CDS             complement(4439..5134)
                     /gene="rsxE"
                     /locus_tag="OO327_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR6019991.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(5138..5758)
                     /gene="rsxG"
                     /locus_tag="OO327_003601"
     CDS             complement(5138..5758)
                     /gene="rsxG"
                     /locus_tag="OO327_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR6019992.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(5762..6820)
                     /gene="rsxD"
                     /locus_tag="OO327_003602"
     CDS             complement(5762..6820)
                     /gene="rsxD"
                     /locus_tag="OO327_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR6019993.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLRFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
BASE COUNT         1787 a         1747 c         1732 g         1655 t
ORIGIN      
        1 tttgctggaa ataatgcatt aagtggaaag ggggagtgag aaatcactcc ccctggtttt
       61 tatacaggga acatgatgaa aaatattctc gctatccagt ctcacgttgt ttatggtcat
      121 gcgggtaaca gtgcggcaga gtttccgatg cgccgcctgg gcgcgaacgt ctggccgctg
      181 aacaccgttc aattttctaa tcacacccaa tacggcaaat ggactggctg cgtgatgccg
      241 cccagccatt taaccgaaat tgtgcaaggc attgccgcca ttgataaatt acacacctgt
      301 gatgccgtat taagtggcta tctgggatcg gcggagcagg gtgaacatat cctcggtatc
      361 gtccgtcagg tgaaagcagc gaatccgcag gcgaaatatt tttgcgatcc ggtaatgggt
      421 catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag agtttcatgt gcggcacggt
      481 ttgcctgcca gcgatatcat tgcgccgaat ctggttgagc tggaaatact ctgtgagcat
      541 gcggtaaata acgtcgaaga agcggttctg gcagcgcgcg aactcattga gcaagggcca
      601 caaattgtgt tggttaaaca cctggcgcga gctggctaca gccgtgaccg ttttgaaatg
      661 ctgctggtca ccgccgatga agtctggcat atcagccgtc cactggtgga ttttggtatg
      721 cgccagccgg taggtgttgg tgatgtgacg agcggtttac tgctggtgaa actgcttcag
      781 ggggcaacgc tgcaggaggc cctggaacat gtgaccgctg cagtctacga aatcatggtg
      841 accaccaaag caatgcagga atatgagctg caagtggtgg ctgctcagga tcgtattgcc
      901 aaaccagaac attacttcag cgcaacaaag ctctgaaata tctcatttcg gcccacaacg
      961 acctgtgggc cgatggctat tactttaagc cttccgctga cagcgcgtct tgtacttccg
     1021 gacgttcagc catacgttgc ataaatgccg caatgtgctc taacccttcc agattcagtt
     1081 tcaccgcgta tgcccagcgc agaaccgtaa acagataggc atcagcaatt gtaaatcttt
     1141 gcccgcagat ccagtgctca tccttcagtg cctcgttcac atattgcagc ttcttatcca
     1201 gctgcgcgcg aactgtcggt ttgtactctt ccggtgtatc tgggcgaaac agaggtgtga
     1261 aacctttatg cagctcggtg gcgatgtaat tcagccattc gatggtttta tagcgggaaa
     1321 tactgtttac cggtgccagc aactggcggt cggggacgct gtcggcaaga tactgcataa
     1381 tcgcaacgcc ttccgtcagc aaagtaccat cgtccagcag caatgcaggc acctgcccct
     1441 tagggttaac ggaaaagtaa tcgtcaccgt tttcgagacg ttttttcatt aaatccacac
     1501 tgacgagcgt aaaatccttt ccgctctcac gcagggtgat atgggaagcg agagagcagg
     1561 cgcccggttt gtagaacaat ttcatcggta actccttttg tctgtggttt aaagtatggt
     1621 agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt tagcggcttg taaaagagtt
     1681 tccctgaatt ttacgctaca gtagctttcg ccgctttgtc tgcagcgtca tcctgcgtca
     1741 tgcggtgcag tttaggcgcg gtcagcagca tcagtactgc aataacggca gtagcgacac
     1801 caatctgcaa gaatacgcga ccatagactt ccagtgacat cagcgggtcg gtaacgttat
     1861 ccggcacagc catcataccc gcaacataac caccaatcag gtttgcaccg gcagtggtca
     1921 ggaaccagct acccataatg aagcccatca gacgctgcgg aacgagttgt gcaaccattg
     1981 ccagacccag accagagatc atcagttccc cgatgctctg caggccatag cttgcgacca
     2041 gccagctgac agacacgata cctgcgtcag acgcgaattt cgcacccagc ggcagaatca
     2101 ggaacgcacc agaacacatc accatgccga ttgcaaactt ggttggcatc ggcagggtat
     2161 cacccatctt gttatagata gcggccagaa tcggactacc gatgatgatc cagaacgggt
     2221 tcagtgcctg atactgctca ggttctacgg ccagacccag aatggagtgc tcaacgttac
     2281 gaatcgcaaa gaagttcagt gacgttggca tctggctgta cagcacgaag aagatgattg
     2341 cttcgagcat caggatgaag gcaacgatca ttttacgacg cgcagcacct ttcatcgcga
     2401 aagcttcttt accgaagata accacaatac cgaaggcaac aacgcccagc gccatacgcg
     2461 caacttcctg attgtgcagt agccaggtgg caacagcgat cagcgccaca acaccaataa
     2521 tggtcagcaa caggttacgg tagttgactg gttcaaagtc tggttttgaa ccgtactgtt
     2581 taacccagcg ttggcagaag gcgaagttaa cgatagtgat cagcaggcct acaacgctca
     2641 acgcaaacgc aacactccag ccgtatttcg cggccagcca cggggtggct atcatagaga
     2701 agaaagagcc gatgttgacg gacatgtagt acatggtgaa tgcaccgtcc agacgtgggt
     2761 cgtttttctc atagcatgta gaaagcagag aagacgggtt agctttaaac aggccgttac
     2821 cgaccgcaat agccgccata cccatataaa caataccggc gtcgtgacca gaccaggcaa
     2881 ccagagcata accaatcgcc agcacaatag cgccgagcat aattacgcgt ttagtaccca
     2941 gtaccttgtc acctaaccag ccgccgatag cgaccagacc ataaaccagg gcactaaagg
     3001 aagagaaaag ggtgattgaa tccgcttcag acatacccag ttgtttaacc aggtaaacag
     3061 ccataattcc ttgtaggccg taataaccaa aacgttccca taactcaatc gagaagatga
     3121 gatagaacgc cttcggttgt ttgaaagcgt tcaaactgac gctttcagtt ggtttttggt
     3181 ttgcagtgga cacttttacc tcttttttta catcccatat taacgggggt gctttctcgc
     3241 gttatgtcta aaaaacatac gcgcaattgt tatagtggga ggggaaacgg caggtaatgt
     3301 tcactatctt gcactttcag acaagtcctt tgtaataatc tgttacatat aactgaggcg
     3361 gggaattcaa ccattccggg aaatgttatc cagcgttaaa tttttcaaaa aacgtaacgg
     3421 cagatttttt cactgcattg agagggggat gatggcttgg cggcgatatg ttctgctatt
     3481 tagcgttttt cctagtgaga tagtccagtt tctgaaaaat agccagtgta atgttttgca
     3541 ggtcaatatt tgttgcgtgt gtaaatttaa atcattttaa cctgattctt gcaatgtttt
     3601 tacattgcca cagcatcgtg atcttgatca cgaatgaata gaaaatttcg ctattaaaaa
     3661 aacaattaac ctgcattttt ggttatccgc aaacggatta cctgatgata acgctaagca
     3721 atggcatcat tgacgttaaa aagatgtttt aaaagaggat aaagaaaggt tatcaatggg
     3781 gtaatcggtg ttaccccttt tcttcagatg tcaactttct ctttgtattc acaaagatct
     3841 tcaataatac aagagccaca gcggggcttg cgggcaatgc aggtataacg cccgtgcagg
     3901 atcaaccaat ggtggcagtc gactttaaac tctgccggaa ccactttcag tagcttttct
     3961 tctacctgtt cgacgttttt ccccggcgca aattgagtac gattacaaac gcggaaaatg
     4021 tgcgtgtcga cagcaatagt cggccagccg aatgcagtgt ttaatacgac gttggctgtt
     4081 ttacgaccta cgccgggcag ggcttcaagc gcagcacgat cttccggaac ctcgccatta
     4141 tgttgttcca gtaatatacg gcaggttttg atgatatttt ctgctttgct gttataaagg
     4201 ccaatcgttt tgatataggt tttcacccct tcaacgccta gttcaagcat cgctgcaggc
     4261 gtattcgcca ccgggtagag tttcgccgtc gccttattaa cactgatatc ggtcgcctga
     4321 gcggaaagca gtacggcaat cagcaattca aaaggcgaac tgaaattaag ctcggtggtg
     4381 ggatgaggat tgttctcacg caggcgagtg aggatctcca gacgttttgc tttgttcatc
     4441 agacattccc tgtttcaccg tttggcaatg cacgttcggc agctgcttca gcacggcgct
     4501 ttttcattct ttcatcaatc aggtattttc ctgccagcat cagtcccaag ccaataaatg
     4561 cacctggtgg cagcatcgcc agcaggaaag gggagtcggt gtggaaaatc tccacgcgta
     4621 atacttttgc ccagctacct aacagcgcat ctgcaccgtc aaacaacgtg ccattgccga
     4681 taatttcgcg tagtgaaccc agcacgaaca tggcgcaggt tgcgcccgta ccaattgaaa
     4741 agccgtccag tgccgaaagc gccggacctt ttttggcggc gaaggcttca gcgcggccca
     4801 caacgataca gttagtgaca atcagcggaa taaaaatccc taatgattga tacaggccaa
     4861 aggcgtaggc gttgatcagc atctgtacag cgctgaccac cgaggcgatg atcatcacgt
     4921 aaatgggaat gcggatctcg gctggcgtcc agtgacgcag cgtcgaaatg gtcaggttgg
     4981 tcagcgtcag taccagcgta gtcgcaagtc ctaaacccag agcgttagtg gcagtggatg
     5041 tgaccgccaa tagcggacaa aggccgagca gttggaccag tgcagagttg tttttccaca
     5101 acccctgaac aataacgtct ttaatttcgc tcacgcttta ttctccacag gcaggaagtt
     5161 gagaaagttg tgccggtaac gtctgagcgt acaatccggc gcgttttacc gcattaacca
     5221 ccgcgcgggg agtaatcgtc gcgccggtga actggtcgaa atcaccacca tctttcttca
     5281 ccgcccagtg cgcatcatcc gcaccactga ttttttttcc cgcaaaatgg gtgagccagt
     5341 cagaaaggcg caattcgatt ttatcgccaa gccctggcgt ttcgtggtgc tctgtcacgc
     5401 gcgtgccaag taccgtgcca ttaaaatcgg ctcccaccag cagctgaatc gcaccggaat
     5461 agccatctgg cgcggttgct tccagaacgg cggctaccgg tttgtcatcc tgtttggcga
     5521 tgtaaacccg atgctcacct ttacctaact ctggcgcagt taccagatag caactctgtg
     5581 cgagcgcatt gttatagcgt tcggctggca gcacctgatc aaataacgtc ttttgttgca
     5641 gactggcctg ttcagcaatt gtcgttttgg tcatctggtt gatggccgca gttaaccctg
     5701 tagaacccgc tgcaaatagt gccagtgtaa tgccgtgttt tcggatagtt ttcagcatgg
     5761 tttacccttt gcgatggccg tagacgcgcg gacgcgtgta gtaatcgatc agaggaaccg
     5821 tgatgttcgc cagcaggacg gcaaaagcca cgccgtcagg atagccgccg aaactgcgga
     5881 tcaaccagac taataagccc gccagcgcgc cgaacatcag ccgaccacga ttggtcgtag
     5941 aagcagtaac tgggtcagtc aaaataaaga atgcgccgag catagtcgca ccagacagca
     6001 gatgaatttg cggtgctgcc agtgtttctg gtgagaacaa ccagcccaac gttgcgcata
     6061 acgccagcgt tactaagaag ctgaggggaa tatgccagcg aatcgctttc tgccatagca
     6121 accacacgcc accagccagc caggcgagat ttacccattg ccaaccagcg cccgccagaa
     6181 taccgctgta gatcggatat tgcataatct gttcaaccga atgacctgcg cggacagagg
     6241 ttttgaatgt atccagcggt gtcgcctgac taatgccatc aatacctaag cgtagcgtat
     6301 tcatatcacc accactggcg gtatgaccgc tgaaaataac ctgtatggcg tcgataaaac
     6361 cagggatgtt gaccgcaatt tcatgtggcg gtaaccagct ggtcatctgc accgggaagg
     6421 agatcagtaa gaccacataa ccaatcattg ccggattaaa cgggttttgc cccagaccgc
     6481 catacaactg tttagcgatg atcaccgcaa acaccgtacc cagtacgacc atccaccatg
     6541 gcgctagggg gggaatactt accgccagca ataagcctgt cagcaatgct gagttatctt
     6601 tcaacgttgc ggctaccgac tgcttgcgta gtttgagtac gagagcttcg gctaacagag
     6661 cactgaccga cgccaacagg atctgaacga gagtacccca accaaaaaac cgcagttgcg
     6721 ctgcgattcc tggcacggct gcgagcaaca ccaacagcat aatgcgcgat gtctggcgct
     6781 ggttatgggt ataaggggag ctagctattc tgaataccat ttagtcctcg tttacaacct
     6841 tttgctgggc ggcttttttg gcctgaacgc gggcaatagc cgcggcaact gcggctttgc
     6901 gcggatcgac ctgttcttct g
//
LOCUS       DAJTSI010000180         6911 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000180 DAJTSI010000000
VERSION     DAJTSI010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6911
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.180"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..695
                     /locus_tag="OO327_003603"
     CDS             <1..695
                     /locus_tag="OO327_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6019994.1"
                     /translation="DAEAICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKT
                     TNQIHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAE
                     LESELSQSINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQ
                     YSTGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVV
                     ACALANKLARIA"
     gene            complement(1034..1804)
                     /locus_tag="OO327_003604"
     CDS             complement(1034..1804)
                     /locus_tag="OO327_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019995.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1849..2283)
                     /locus_tag="OO327_003605"
                     /pseudo
     CDS             complement(1849..2283)
                     /locus_tag="OO327_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(2295..2516)
                     /locus_tag="OO327_003606"
     CDS             complement(2295..2516)
                     /locus_tag="OO327_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019996.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2517..3200)
                     /locus_tag="OO327_003607"
     CDS             complement(2517..3200)
                     /locus_tag="OO327_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR6019997.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3264..3575)
                     /locus_tag="OO327_003608"
     CDS             complement(3264..3575)
                     /locus_tag="OO327_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6019998.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLVSVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(3572..4474)
                     /locus_tag="OO327_003609"
     CDS             complement(3572..4474)
                     /locus_tag="OO327_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCR6019999.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVNVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4914..5873
                     /locus_tag="OO327_003610"
     CDS             4914..5873
                     /locus_tag="OO327_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HCR6020000.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5873..6268
                     /locus_tag="OO327_003611"
     CDS             5873..6268
                     /locus_tag="OO327_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCR6020001.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6451..6831
                     /locus_tag="OO327_003612"
     CDS             6451..6831
                     /locus_tag="OO327_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR6020002.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
BASE COUNT         1888 a         1786 c         1675 g         1562 t
ORIGIN      
        1 ttgatgcaga agccatatgt gaagcggctt cacgaccatc aatgcgtttt gtccagccaa
       61 gaactgagac ccagcaagca ataagggcat tacaccgggt cagagaatcg ctcatcagag
      121 ataaggtgaa aacaaccaac cagatccacg gtttcctgct ggaatttgga atcagcctgc
      181 cgacagggga tgccgtaata aaacggttgt ctctcgtatt ggctgaacac gaaatccccg
      241 aatatctgag ccgccttttg gtgaggttgc atacccacta cctttacctt gttgagcaaa
      301 tagcagagct ggaatcagaa ttaagtcagt caatcaacgc tgatgatacc gctcagcgca
      361 ttatgacaat tcctggtgtt gggccgatta ctgccagctt actatcgtcg caacttggtg
      421 acgggaaaca gttttcatgc agcagagatt ttgcggcttc cacagggctc gttccccggc
      481 agtatagcac tggaggaaaa tcgactctgt tgggtatcag taagcgaggt gataaaaatt
      541 tacgccggtt actggttcag tgtgcccgct cattcatgat gcagcttgaa cgccagcatg
      601 gaaaacttgc tgagtgggtt cgggagcaac tcaacaagaa gcattcgaat gtggttgcct
      661 gcgctctggc aaataaactg gcccgaattg cctaggcaat cactactcag caaaaagaat
      721 atcaggcctg atatatagat gctgacaaaa ctcaattagc ttattgcaaa caataagata
      781 caagttaccg tctggttttg cgaagattga ttattgatga catgaacggc aaatcgacct
      841 gattaaaaac ctaacagggg aaaatggctc actgaagccg aggggatttt taggattatc
      901 aggtgcggaa ctcatcgagg cgcgggcatc ccttgcccac agagacgccg gatagattta
      961 agcaagccca ccattaatca aaaatcagtg ttgcaaaaac gggggtgacc atagattttc
     1021 cccgccatcg tcatcatttc cggatttttg ccagggcatc cgccatgctg cggatttcgg
     1081 cccgggcttc ccggacaact tcccgcagaa caccacggtc aaaccaccgg cggggcggtt
     1141 catccggtat acgcagcaca tcgcaggcca gcccactgga caggatatgc tcaatacaat
     1201 cgctgaccag atacatttcc accgccgaaa aacgtgcgcc ccggttcagg atggcatcga
     1261 gcaccacggg caggttttca tcgtcttcat cgagccagca ggggtgatcg ctttccttca
     1321 cgaccaggcg aaagcggcgc gtctggcggg tcagcaacgt ctgaccgctt gtggtcagcg
     1381 ccgcccggat acgccgcgca tcttctgccc cccggcaact gacactgcat actgcatcgc
     1441 gcaccgcatc aagaactgcc ctgatttcgt cttcggtcag ccattcaccg gcatacggga
     1501 aagacacctg cgttccgtcc gccggaagta ccggcagcga ctgccactga ctgccggaca
     1561 gaacaagttc cgctccggtt ccggtcagtt tctgaatggc ggaggccagg tgcggcggca
     1621 gcacgccatc gtggcggtta acgagtaacg tcgccagccg gggcgcatcc agccgcacgt
     1681 aaatgctgat ccagcccgga tagttcgggt tacattggtc aacatccgtc tcttccggcc
     1741 agccctgcgc cagcagcagg ttacgggcgt gacgacaaat cagagcattc agcgtttcat
     1801 tcataattca tgaactccct gtatcgccgc ccacaggcgg cgataacatc agtcgttttc
     1861 gtgtgtgttg tttttcagca ggctgcgggc ctgatcaacc atcgcctgtc ggcgggtaac
     1921 gtattccggg agggggaaac cgtgatcccg ggcagcccat tcacgtttcc acagtgcctg
     1981 ttcagcgatc tgagtcaggg tctccagcag ttcagcaggg acggtcacct ggcggttttc
     2041 gcgcacgttg ctgataatct ccatcgccgc ggcggaagaa ataccggact caatccgcta
     2101 gtcttccggg atgtcctcca ggtgcgccag gacggtcctg atttcatgat cggacaggga
     2161 aatatccatt ccgtcagcga gggcctgaat ctcttccggg gaccagataa ccagcatcag
     2221 cggcgtgtct cccgaatact ttgctgccag cgcccggcat agcgtttcgc atgttccgta
     2281 catactctct cccctcaatc tttgaagcag gaatccagtt ccagaatttc ggtatatgcc
     2341 tccatcaaga ggcggtttgc gcgaaacaac gtctgacgca ccagacgttc cggcagggtg
     2401 tcagcattaa agctgtaccg ccgctccatc tcattcatgg ttgtacgcag gtcgcacatg
     2461 gcattggcca cctccgccac ttcggcgcga agtttttcgt gtccggcata gttcatttac
     2521 gcagcctccg gcataaacca gtcatcattc gcggccccct gctgcatggc ccgctgtacg
     2581 gcagccagtc gttgctgtcc ggcacggtga tactgctcaa gcagctcgat accgatatac
     2641 cggcgtccgg actggagggc ggcgacgcag gttgaaccgc tgcctgcaaa cgggtccagc
     2701 actatcgcat tcgggtgtgt gaagctctca atcagcggtt gcaggctggt aacgggcttt
     2761 tccgtcgggt gatggcggtt gcccgaatat ttccagccca acacgtccgg cagcgggttt
     2821 tgtggcagac gtggacggcc ttttgccagg atgtaggcgc attcgtggcg atagcccaca
     2881 tatgcggcct tcgatgtgta gtttttggtg aagaccaggt gaccaacaac gctgaatccc
     2941 gcatttttcc aggcagacat aaagcgatcg acgcggttcc agccatagaa gctcaccatc
     3001 agcgcgtctt ttttgagtac gcggtacatt tcattacagg ccggttgcag ccattcatca
     3061 gttttatcac cagcaatggt acgcccctga cggtcacgaa aaccgacgag atatggcgga
     3121 tcggtgagga tgaaatcaat ggcgttatca ggaatacggg ccataacatc gatgcagtta
     3181 ccgaggacaa aacgggacat aacagatcct tctcttacag agggcacccc tgtggtgccc
     3241 gttccccgct gcctgtgggc tgttcatcgc ttccggtaac gggtcaggcc gaccgtggaa
     3301 taccgcagcc gtcgtctttt tgacagcaag gttatgccgc gaaagcggct tgaagggcgt
     3361 tcatcagaaa agggcgacgg gctggcgtac actgtcaccg acaccagccg ggagcggtaa
     3421 aagcgcccgt cccgtgcccg ccggacgcgg gacactgacc acaggcagcg tttatgccgc
     3481 gccgcgtcag ccggcgcagt aaaaagagga gcacggcgac atcctttgtc ggaaaagaaa
     3541 cgggcggttg cccgcccgtc tccgtcatac ttcatccgcc ataatgcgcc acattgtcga
     3601 ctatccacgc aggtccggtg acttccggct gctcatcgtc atctgtcggg gaagaccatt
     3661 caagttcccc ccacagcaca ttgacgagct ctccacgctc atacaactgc cagccacaga
     3721 aatcacagcc ctgttcggca taccagtgct ccagcgtgca gctgaaacgg cggcttaatt
     3781 ctgcaataac gtcgctttcc ggctgacacc acggagtgtc gaaatccacc tgaatgaagt
     3841 tttctccctg actgctgata ttcaggtcat atccacaagg ccacttcacg ccataccgtt
     3901 ccgtatacca gtgatatgca gaaggaacac cgttcagaac accaccatta aagccgttca
     3961 cttcaacatc caggcgggtc ggcagaaccg tcagcaggtc aaacggcatg gttttttccg
     4021 gcactacgtt gtcacacaga cggttccacc acaccgatac atcctcgtta ccccatatgt
     4081 cacaccagtc acctcttttt gcggtgaaat gcacagtgat ggtttccctg gcatcatccg
     4141 gtaatccttc ccaacgacgc tggccagtac cgctctgtaa ccagagttca tgcagcatca
     4201 ggcagttttg ttcatccagc aacacaccgt tctgcaggtg tgtcagccag cgggtgaacg
     4261 cgatattttc cgtcgataca gcaccacgtc cggcagccgt cactccgggg cactgttcag
     4321 agcggatatt ttcagtgatc tgcagaagtc cggcacttcc ggccagaaac agctgaatac
     4381 cttcatttgt ggcgcggcga taaagcggtg tgactgcacc gctggccagt cgtttaatct
     4441 cagcgatctg tgccggctca ccaggaaaat acatacgatt cgagcaccag tttggcatgg
     4501 gaattctcct ctcacatgcg gcctccatca ggccgtgccg cacctgtaac cgggcgaccg
     4561 catacgaatg caggtgcggc cccgaagccg cagcgcgtgg aggaggaatg acgaaacgtg
     4621 accgggtgag gaggccggcg acagcgacac acttgcatcg gatgcagccc ggttaacgtg
     4681 ccggcacggc ctggttaaat agtatccgtc tcagttcgtg gcgcaaaatg agaaaccaat
     4741 aagaactcaa aatgaaccac aacaagaaca taaagagact ctaaaagagc aacaccgttt
     4801 gacatagact caaaatgagt cacaataaga aacaataaaa aactcaaaaa gatgcaccga
     4861 cataaaggat gctcaatttg agataaacac aaactcaaaa cgaggcaaaa agaatgctaa
     4921 aagtatcatg cgatgatggt tcaactaacg taaaactggc ctggcttgaa gatggcgagg
     4981 taagaaccag tctgtcagga aacagcttta aagaaggctg gaacccggga ctgtttaatg
     5041 ccggcaaagt ttacaattac gtcgtggatg aaaaaaaata cacctatgat ttgggaagca
     5101 ctgctgtcat aggtacaaca catgtcagtt atcaatacag caccaccaat ctgcttgcca
     5161 tacatcatgc actgctgaca tccggccttc agccacagga tgtagaactg acagtaacat
     5221 tacctgtgac cgaattcttt gataatgaca atcaaccaaa tgaagaaaga atagaaagaa
     5281 aaaaggcaaa tgttctccgt gagatatctc tgaataaagg ggaaacattt aaaattaaaa
     5341 aagttaatgt aatgcctgaa tctttgccag ctgcatttga atcactaaaa aaagataaag
     5401 taaacaaact tgaacgcagc ctgattatcg atcttggagg aaccactctg gattgtggtt
     5461 taattctcgg tgcatttgaa ggcatttctg aaatccgtgg ctattcagaa atcgggacat
     5521 cccgcatcac tcataccgtc atgaatgcgc tgacgaaagc ctccacacca tgtaattatt
     5581 ttattgctga tgagttaata aaaaacagac acgataatga atatctccag acattaataa
     5641 atgatgtagc tgaaataaaa aacatttctc atgtaataga cagagaagtg aaatctctgg
     5701 cagaaagcat caggcaggaa atttcaacat tctcaggaat gaaccgaatc tatctcactg
     5761 gcggaggagc cgagcttatt taccctcaca taaagcaata tttcccgaat ctgaaagtga
     5821 acaaggtgga tgaacctcag tttgcattgg tgaaagcaat ggtgcatgcg taatggaaag
     5881 cagtgatcca aaaaaaagaa agaaagttgt ggcatacctg cacccggcac tttatccgca
     5941 ggacaacctg acacaacaaa caatagactc tctcccagta caaatgcggg gagatttcta
     6001 cagacaatcc cttatttgcg gagcagctct ttattctgtt gccccccgac tgttaacgct
     6061 aatcagcgtt tttttcagtg agaaaatcac tgcagaaaat ctggtgaaac ttatagaaca
     6121 aacaacagga tacacatcca catcaattga tataagtgtg ttaaaaaaca tcatagaggc
     6181 atcttcagaa aacaaatcag agagcatcac ttcaaaagat gatttcgaag aacaaaccag
     6241 acgcaatctt tctatgttaa aaaaataata aaagaagagc aggttcagtc aattacctgc
     6301 tcttctttta tacatactaa aaaatcagat ttttacgaat attcagatta tcataaaaaa
     6361 tgcaacagac aggaatgcaa taattttgca ttatctcgtt gcttgtaatg caaatatgca
     6421 ttattgtcag tgtgtgacaa taacagagag atgacgatgg cgaccattaa cgcacgtatt
     6481 gatgatgata taaagaacca agcagatgaa gtgctgaagc tgatgaatat cagtcaaact
     6541 caggctattg cagcttttta tcagtatatt actgaacaaa agaaactgcc atttgtgatc
     6601 acctcaattg taaaaacgcc gcatgattta ttacgtgaaa gtacagacat gttagctgaa
     6661 gctcttgcag tgatttccaa tctacaggtg tggactgaac agcaggatgg gattggaaaa
     6721 gcaaagctaa tggagtatta ccgccgcctc gacgcactgt actgttgtgc aaaagagaaa
     6781 ataggtcttc tttcagataa ccgggatgct gaactaggct gtgtccctta atcatctgag
     6841 ttatagtaac tgtctgttta cacaacatat tgaactatgg ctcgctacga ccttcccgat
     6901 gaggcatgga c
//
LOCUS       DAJTSI010000181         6795 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000181 DAJTSI010000000
VERSION     DAJTSI010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.181"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            240..2672
                     /locus_tag="OO327_003613"
     CDS             240..2672
                     /locus_tag="OO327_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR6020003.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2739..4208
                     /locus_tag="OO327_003614"
     CDS             2739..4208
                     /locus_tag="OO327_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR6020004.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4208..5869
                     /locus_tag="OO327_003615"
     CDS             4208..5869
                     /locus_tag="OO327_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR6020005.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6090..6431
                     /gene="symE"
                     /locus_tag="OO327_003616"
     CDS             6090..6431
                     /gene="symE"
                     /locus_tag="OO327_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HCR6020006.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6478..>6795)
                     /locus_tag="OO327_003617"
     CDS             complement(6478..>6795)
                     /locus_tag="OO327_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020007.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
BASE COUNT         2003 a         1612 c         1554 g         1626 t
ORIGIN      
        1 tttcttgttc gaaagaaacg caactataag atttttatat attttgttag agttgatatg
       61 gttcctgtaa cttctgttta tctgtctctg atatgcccag attaattcaa gcaagatata
      121 acagagatat caatgtgatc tctgctacgt actttacgtt gaaacaccgc cctaaatcat
      181 agccaggcta ttgattttat gtacaatcag aatcgtcctg ataaaagcga gaacccccca
      241 tggcggaact gaacctaagt aacctgacgg aagcagacat cattaccaaa tgcgttatgc
      301 cagccattct caatgcaggc tgggacaaca caacgcaaat cagacaggag gtcaaactcc
      361 gggacggtaa agtcatcgta cgtggtaaag ttgcggcacg cagaacggta aaatctgctg
      421 acatcgtgct gtaccacaag ccaggcattc ccttagccgt gattgaagca aaagccaaca
      481 aacatgaaat tggcaaaggg atgcaacagg gcattgaata tgcgcgcctg ctggacgttc
      541 cttttgtttt tgccaccaac ggtgatggct ttatcttccg cgatgccacc gcagccgaag
      601 gtgaatgcct tgaaaagcaa ataacgctgg atgacttccc ctcccccgct gaactctggc
      661 aaaaattctg cctctggaaa ggatacacac aagctcagct tccggtgatt actcaggact
      721 attacgacga tggcagcggc aaatcgcctc gttattatca acttcaggca atcaacaaaa
      781 ccattgaagc cgtctccaac gggcaaaacc gcgttctgct ggtcatggcg accggaacag
      841 ggaaaaccta taccgcattc cagatcatct ggcgcctgtg gaaatcaaaa aataaaaaac
      901 gcattttgtt ccttgccgat cgcaacattc tggtcgacca aaccaaaaat aatgatttcc
      961 agccatttgg tacggcaatg accaaagtca gcggacgcac cattgatccc gcttatgaaa
     1021 ttcacctcgc gctctatcag gctataactg gcccggagga agaccaaaaa gcgtttaaac
     1081 aagtcgcacc agatttcttc gatctgatcg tgatcgacga atgccatcgc ggcagcgcat
     1141 ccgaagacag cgcctggcga gagatccttg attatttcag ttccgccacc caaattggct
     1201 taaccgccac gccaaaagag acgcatgaag tctccagcac ggattacttc ggcgatccgg
     1261 tttacgtcta ctcactaaaa gaagggatcg aagacggctt cctcgctcct tataaagttg
     1321 tccgtgttga tattgatgtc gacctgcaag gctggcgccc aaccaaaggg caaactgact
     1381 taaacggcga agtgattgac gatcgtatct ataaccagaa agatttcgat cgcacgatgg
     1441 tgatcgacga acgcacagaa ctggttgcca gaaccattac cgactacctc aagcgcacca
     1501 atccgatgga taaaaccatc gtcttctgta acgacatcga tcatgcagaa cgtatgcgcc
     1561 gcgccctggt taatctcaac ccgcagcagg tgaaaaagaa cgacaaatac gtcatgaaaa
     1621 tcaccggcga tgatgaaatt ggcaaagctc agttggataa cttcatcaac ccgaaaaaac
     1681 cgtacccggt tatcgcgacc acttcagagc tgatgaccac tggtgtggat gctaaaacct
     1741 gcaaactggt agtactagac cagaacatcc agtcgatgac caaattcaag caaattatcg
     1801 gtcgtggtac acgcatcgac gaacgttacg gcaaactctg gtttaccatc ctcgacttta
     1861 aaaaagccac cgaactgttt gccgatgagc gtttcgatgg cattcccgaa aaagtcatgg
     1921 ataccacacc agaggatatc gccgatccag aatctgattt tgaagagaaa ctcgaagaaa
     1981 tcagcgaaca tgacgacgaa caggtaacag gcgttgatga accgcctgcg ccaccatacc
     2041 aggttaccga taccgatgat gtcggcccat ttccggaaga agacgagaag aaaatccgca
     2101 agtttcacgt caacggtgta gcggtgggcg ttattgcaca gcgcgttcag tattacgacg
     2161 ccgacggtaa actggttacc gaatccttta aagattacac ccgcaaaaca ctgctcaaag
     2221 aatatgcctc gctggatgac tttacccgca agtggcagga cgccgatcgc aaagaagcga
     2281 tcattcacga gctggagcaa caggggatca tctgggaagt actggcagaa gaagtcggta
     2341 aagatctcga cccgttcgac atgctttgcc acgtagtgta tggtcagccg ccgttaaccc
     2401 gcaaagagcg cgccgagaac gtgcacaagc ggaactactt cacaaaatac tctgaagcag
     2461 cgcaagccgt gctcgataat ctgctggata aatacgccga tgcgggcgta caggagatcg
     2521 aaagtattca ggtgctgaaa cttaaaccat tcgacagcat gggcacctta ccggagatta
     2581 ttaaaaccgg atttggcgac cgtaacgggt ataatcaggc gctcagcgag ctggaaaacg
     2641 aaatctacca attaccgccc cgctctgctt aacaactctc ttttgcgcct tctgcaacca
     2701 tcatgttgca gaaggccttg tttttcatgg aatacaacat gtctatcagc tcagtaatca
     2761 aatcattaca agatattatg cgcaaagatg ccggtgtgga cggcgatgcg cagcgtctcg
     2821 gtcagctctc ctggctgctg tttttgaaaa tcttcgatgc ccaggaagag gcgctggaac
     2881 tggagcagga taactatcaa tatccgatcc cacagcgtta tttgtggcgc agttgggccg
     2941 ccaacgcgca gggcattacc ggtgattccc tgctggaatt cgttaatgat gatctgtttc
     3001 cggcgttgaa aaacctcact gcgcctatcg ataaaaaccc acgcggctac gtggtgaagc
     3061 aggcgttcag cgatgcctat aactatatga aaaacggtac gctactgcgc caggtgatca
     3121 acaagctgaa cgaaattgac tttaccagcg ccagcgaacg ccatctgttt ggtgatattt
     3181 acgaacagat ccttaaagat ctgcaatctg cgggcaatgc gggcgaattc tatactccac
     3241 gcgccgtcac tcgctttatg gtggatcgcg ttgatccgaa actcggcgaa tccattatgg
     3301 acccggcctg cggtacgggc ggttttcttg cctgcgcgtt cgatcatgta aagaacaaat
     3361 acgtgaagag cgtcgccgat catcagacac tgcaacaaca gatccacggt gttgagaaaa
     3421 aacagcttcc gcacctgctg gcgaccacca atatgctgct gcacggcatt gaagtaccgg
     3481 tacaaattcg tcacgacaac accctgaata aaccgctttc ctcctgggat gagcaactgg
     3541 atgtcattgt taccaacccg ccgtttggtg gcacggaaga agacggtatt gagaagaact
     3601 ttccggcaga gatgcaaacc cgcgaaacgg cggatctgtt tctgcaactg attgtggaag
     3661 tactggcgaa aaacggtcgt gcggcggtgg tattgccgga tggcacacta tttggcgaag
     3721 gtgttaaaac caaaatcaaa aagctactta ccgaagagtg caacctgcat accatcgtgc
     3781 gtttaccgaa tggtgtgttt aatccctata ccggcattaa aaccaacctg ttgttcttta
     3841 ccaaaggtca gccaaccaaa gagatttggt tctatgaaca tccgtatccg gcgggcgtga
     3901 aaaactacag caaaaccaag ccgatgaagt ttgaagagtt tcaggcggag atcgactggt
     3961 ggggtaacga ggccgatggt tttgccagcc gcgtcgagaa tgagcaagcg tggaaagtca
     4021 gcattgatga cgtgattgcg cgcaacttca atctggatat taaaaaccca catcaggcgg
     4081 aaaccgtcag ccatgatccg gacgaactgt tagcgcagta tgcaaaacag caggcggaga
     4141 tccagacgct gcgtaatcaa ctgcgcgata ttcttggcgc tgcgctgtct ggcaaggagg
     4201 ttaactaatg agtgtggaaa agctgatcgt tgatcatatg gaaacctgga cctcggcgtt
     4261 gcaaacccgt tccaccgccg ggcgcggcag ctccggtaag attgatttgt atggtattaa
     4321 gaaattgcgt gagctgattc tggaactggc ggtgcgcggt aaactggtgc cgcaggatcc
     4381 gaatgatgaa ccggcgtcgg acctgctgaa gcgtattgcg gcggaaaaag cagagctggt
     4441 gaagcaaggc aaaattaaaa agcaaaaacc actgccagaa attagtgagg aagagaagcc
     4501 gtttgaattg ccggagggat gggagtgggt gcacctccct gatatttatt gctcaatttc
     4561 tgagtcatct agaaaaataa aatcctccga aatactacct gagggaaaat atcctgttat
     4621 cgagcaaagc caagaattta tttctggtta ttgcaacaat gaatgtctgt taataaagtt
     4681 aaataatcca gttatagttt ttggtgatca cacaaggaat attaaattta ttgattttga
     4741 ttttgttgtc ggcgctgacg gtgtgaagat tttatcacct atattaattt gtgagcgttt
     4801 ctttttttgg cagttacgat catttaaatt agatgtaaga ggatacgcga gacattttaa
     4861 agtattaaat agctgcttat ttgcattacc ccccattgca gaacaagagc gaatagttga
     4921 aaaagttagc tctttgatgt ccctctgcga ccaactagaa cagcaatccc tgaccagtct
     4981 ggacgcacat cagcaactgg ttgaaaccct gttgggaacg cttacagaca gccaaaacac
     5041 cgcggaactg gctgaaaact gggcgcgtat tagcgagcat ttcgacaccc tatttaccac
     5101 cgaagccagc gtggatgcgt taaaacagac cattctgcaa ctggccgtaa tgggcaaact
     5161 tgtgccgcag gatccgaatg acgaacccgc ctctgaactg ctcaaacgta ttgcgcagga
     5221 aaaagctcaa ttggtgaaag aagggaaaat aaaaaaacaa aaaccactgc cgccaattag
     5281 cgatgaggaa aaaccgtttg aacttccgga agggtgggaa tggtgtcgta ttggtaatat
     5341 agttaatatt aaatctgaat tagtatcacc aaaagattat ttaaatttat atcaagtagc
     5401 acctgatatt attgaaaagg gcactgggag agtgatatca aagagaacag tgaaagaatc
     5461 aggagtcaaa gggccaaata gtcgctttta taaaggacaa attgtttatt ctaaaatccg
     5521 cccgtcatta tccaaagttt ttttagcaga gtataatggt ctttgcagtg ctgatatgta
     5581 tccattagac tgttacatta atccaaatta cttattgaaa tatatccttt ccattccatt
     5641 tcttatgcaa gtcaaaaaag cagagaacag aataaaaatg ccaaaactta attctgacag
     5701 tttttacaat attattgttg cgattccccc ctataatgaa caacaagcaa tatttgataa
     5761 gataaattct attgaagcag tatgcaatgg tcttatttct tatattggga tttatcataa
     5821 aacccaactg catcttgcag acgcactcac tgacgcagcg ataaactaat tccctcgccc
     5881 tcacaaaacc aataaagcgg ctaaatagcc gctttattca catcaccaaa atttatattt
     5941 cacgctaatt ttcatcttat tttcattaat ttttagctgg actttccctg tatttactga
     6001 tgatgtatcc aggtatttag cacggtgcgg atgtgcgcca acacactcgc accgctaatc
     6061 acaatcacta tttatagaga atagtaatca tgactgacac gcattctatt gcacaaccgt
     6121 tagaagcaga agtctccccg gcaaataatc gtcaattaac cgtgagttat gcgagtcgtt
     6181 acccggatta cagccgtatt cccgccatca ccctgaaagg tcagtggctg gaagccgccg
     6241 gttttgccac cggcacggcg gtagatgtca aagtgatgga aggctgtatt gtcctcaccg
     6301 cccaacccgc cgccgccgaa gagagcgaac tgatgcagtc actacgcaag gtgtgtaagc
     6361 tgtcggcgcg taaacaaagg caggtacagg aatttattgg ggtaattaca ggtaaacaga
     6421 aagtcgcctg atacattgac ccggtagcgc cagtgctacc gggtattatt cgccgcgtta
     6481 taccaacgcg ctctccatct gtttttcaaa tgcctgacga attaatccca taatgtaagg
     6541 aagttgtgcg aggtcgctga aaccaatttc tacatcgcca ttaccccagc gcccaacatt
     6601 tgtcacatct ttggcaatac ctttcggatc gaccagttca tgaaactgca tattaagcga
     6661 caatcgcagt cggctttttt gcggcacaac atcaacaaaa ttcgtttccg ctttaaacgc
     6721 aatatatagc ttcaatactt cctgcgtgat ccctgcgtcc aggcgcataa cttcatcgcg
     6781 aagatgatcg aataa
//
LOCUS       DAJTSI010000182         6559 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000182 DAJTSI010000000
VERSION     DAJTSI010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6559
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.182"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            154..504
                     /locus_tag="OO327_003618"
     CDS             154..504
                     /locus_tag="OO327_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR6020008.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            557..952
                     /locus_tag="OO327_003619"
     CDS             557..952
                     /locus_tag="OO327_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR6020009.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            993..1736
                     /gene="cmoA"
                     /locus_tag="OO327_003620"
     CDS             993..1736
                     /gene="cmoA"
                     /locus_tag="OO327_003620"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR6020010.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1733..2704
                     /gene="cmoB"
                     /locus_tag="OO327_003621"
     CDS             1733..2704
                     /gene="cmoB"
                     /locus_tag="OO327_003621"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR6020011.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="OO327_003622"
     CDS             complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="OO327_003622"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR6020012.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5323..6423)
                     /locus_tag="OO327_003623"
     CDS             complement(5323..6423)
                     /locus_tag="OO327_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR6020013.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
BASE COUNT         1505 a         1716 c         1554 g         1784 t
ORIGIN      
        1 taatgggctt ttaatcctat cgtttattgc ttgcatagta gaatctaacc atccatcact
       61 catagctaac cctcggaaat cttacatttc tgtctatcat aaccactagc atcaccttta
      121 ggaaccatct gaaacgggag gtgtatttag accttgctaa tcttagacag ccgcccggtt
      181 catgcatcac gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt
      241 ccggtacatg ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat
      301 atgacgcaaa accgggaatt atttcaggtc tggttacaaa aattagcgca gtggcatcag
      361 accactacgc cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta
      421 cataccctgt gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt
      481 ccacagcaat cttctctttt ctgaatttgc cacctatcat agacaggtgc catcggccat
      541 tttaaaggga gtttgtatgg taagcgcgct atatgccgtt ttaagtgcgt tgttattaat
      601 gaagttctct tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg
      661 tggttttagc gaactgcaaa gcgctatttg cattcatggt aacgcggtgg aatatattcc
      721 catcgcgatt gtgctgatgc tgtttatgga aatgaatggc gcagaaacct ggatggtgca
      781 tatttgcggc atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg
      841 tctgtttcgc tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt
      901 gctggcgaat ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat
      961 acgccacttt ctttttcccg gatttttacg ttatgtctca ccgcgacacg ctattttctg
     1021 cccctatcgc cagactgggc gactggacct ttgatgaacg ggtcgctgaa gtcttcccgg
     1081 atatgatcca gcgttccgtt cccggctatt ccaatattat ttccatgatt ggtatgttag
     1141 ccgagcgctt cgttcaacct ggtacgcagg tttacgatct gggttgttct ctgggcgcgg
     1201 cgacgctctc ggtgcgtcgc aacattcatc atgataattg caaaattatt gccatcgaca
     1261 actccccggc gatgattgaa cgctgccgtc gtcatattga cgcctataaa gcccctacgc
     1321 cagtagacgt cattgaaggt gatattcgcg atatcgccat tgaaaacgca tcgatggtgg
     1381 tgctgaattt taccctgcaa ttcctggaac cttccgagcg ccaggcatta ctggataaaa
     1441 tttatcaagg actgaacccg ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag
     1501 atgccaaagt tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt
     1561 acagcgaact ggagatcagc cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt
     1621 ccgtggaaac ccataaagca cgcctgcata aagccggttt tgagcatagc gagctgtggt
     1681 tccagtgctt taactttggt tcactggtgg cattaaaagc agaggacgct gcatgatcga
     1741 ctttggtaac ttttattctc tgattgccaa aaatcatctt tcacactggc tcgaaacgct
     1801 gcccgcgcag attgctaact ggcagcgcga gcagcagcac gggctgttta agcagtggtc
     1861 caacacggtg gaatttctgc ctgaaattaa accgtatcgt ctggatttat tgcatagcgt
     1921 aaccgccgaa agcgaagagc cactgagcac cgggcaaatt aaacgcattg aaacgcttat
     1981 gcggaacctg atgccatggc gcaaagggcc gttctcactg tatggcgtca acatcgatac
     2041 cgaatggcgt tccgactgga aatgggatcg tgttctgccc catctttctg atttaaccgg
     2101 acgcaccatt ctggatgtcg gctgcggcag cggttatcac atgtggcgca tgattggcgc
     2161 aggcgcgcac ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc
     2221 ggtgcgtaaa ctactgggta acgatcagcg cgcacatttg ttaccgttag gtattgaaca
     2281 gcttccggca ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt accatcgccg
     2341 ttcgccgctg gagcatctct ggcagttgaa agatcaactg gtgaatgagg gtgaactggt
     2401 gctggaaacg ctggttattg atggcgatga gaacacggtg ctggtaccag gcgatcgtta
     2461 cgcgcaaatg cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa actggctgaa
     2521 gaagtgtggt tttgtagata tccgcattgt ggatgtgtgc gttaccacta ctgaagagca
     2581 gcgacgcact gaatggatgg ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc
     2641 gagtaagaca gttgaaggtt atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc
     2701 gtaaaggtct ggtattactg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccct
     2761 tgctaattca atctattgca gggactatgt aggcctgata agcatagcgc atcaggcagt
     2821 tttacgtttg cataacctca gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac
     2881 agcaggctta tcaaatgccg ttaacttcgg tgcgttaccc gtatattttt cgatatacac
     2941 cagcgcagag ttaccggcac aggcatttgc ccgctgcgag ctggggatat ccgccgttaa
     3001 cacgttcgca ctgccgtttt tacacaagcc gttttccaga tctggccatg caccttcatg
     3061 cacgcatacc acgccttttt tgatcccgtc agtcaccacc gcgcctgtca aaatctgacc
     3121 acgtttgttc cagacacgca ccagatcgcc attcgcaata ccaaatcgag cagcatcttc
     3181 ggtgtgaata gtgatcggtt cacgatcggc aaccgcatat tttttacgca gttccgcata
     3241 gttaagctga ctgtgtaaac ggtgtgccgg atgtgcggtc agaagctgca actgcttctc
     3301 gtcggcggta cccttccact catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt
     3361 atagccaaat ttttccagcg ttttggagta aatctcaatt ttgccgcttg gcgtacccag
     3421 cgcatttttc accggatcgg cgcggaaatc accataacga acgtactgtt cgttcttctc
     3481 gctgcggcgc atttcgatca gtttattttg ctgccagaag gcattaaaca ttggcatagt
     3541 gacgcgttgc gcacgggccc ctttctgagc ggcatcatag aaaaatttca gccacgccat
     3601 ttcatcttta ccttcggtat agatctcttt tccgccaggt ttgagtaatt ccgcaagatc
     3661 ggcaaacacg tcaaaatcgt tacgcgcttc aaattgcgga gcaacagcct gcttcatcgg
     3721 cacaatatgc tggttgctgt aatcaccggt catcgtcaag tcattgcgct caaacgatgt
     3781 ggtgatcggt aatacgatat ctgcgtgttt ggctgctgcg gtccagtagc attcagaaac
     3841 gacgatcatc tccggtttct gccaggcttt gatcagacga ttagtatcct ggtgatgggt
     3901 aaagttaccg ccacccgccc accagatcat tttgatattc ggatacgttt gttctttacc
     3961 gttatgctga tatttgccgc caggattttc cagcgcatcg acgatacggg caacaggaat
     4021 cgccgtcatt ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat
     4081 ttccggcagc acgccgccaa cacgcgtcgg gttgccacca ttggagtaat gataagagaa
     4141 accaaaaccg ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat
     4201 ccagtgtttc tgttcaccat attgctggcg ctgaattccc cagcctgcca tcagcattgt
     4261 gcgattcgcc gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat
     4321 ttccgccgtc cagaccgcgc tcttcggcgt attatcgctc ttacctgtca gatactcttc
     4381 aaactgcgga tacccggtag tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt
     4441 catcagggta tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat
     4501 ccaggtggca ttatcgccaa agaattcgat ggtttcggag cggataggat caatggcaat
     4561 cactggtttg ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact
     4621 gctccaggca atttttaagg tattcagcgg gttcattccc cacagcacca caacctggct
     4681 gttttccaga atcagcggcc aggaggcctg ctgttcatac acctctacag aaccgaccac
     4741 atgcggcatg atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc
     4801 gcccgccagg ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga
     4861 gcgccagccg taagatccgg caaaaatggc cgatggtccg ttagctttac gaatacgctc
     4921 atgttgctca tgaatcagtt ttaatgcctg ttcccagctc acctgtacat aggtatcttc
     4981 gccacgacct ttcgccggtt gcagtggatt gtcgagatag ctttttctca ccatcggatg
     5041 ctgaatacgc gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat
     5101 ggttttcgcc agcgcgcctg tcgaagaaac aatcttgccg tccttcactt ctacgctcat
     5161 cgctccccaa cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcggggag
     5221 cggtgctgcc gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc
     5281 acgtcttgtt aatgccataa cttcctccct gatcaacgag gatcactgtt tctcggtaat
     5341 atctttggcg ttgtactgga aataccgcgt taaaatgtcc ggttcgtttt cgctcatgct
     5401 ggttcgtgcc cccattcctt tggcaatgga cggccacgca ttgacggtgt aatggtcggc
     5461 ggcaatagag gcatgacaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca
     5521 tagcggttta cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg
     5581 ccattgattg ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc
     5641 ttcttcactt aatgtggcta gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc
     5701 actgccctgc atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac
     5761 gacggtgaga tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa
     5821 aggataaata tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc
     5881 cgcgttgtca tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat
     5941 acaggtttcg ccgccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga
     6001 ggcaatatcc atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt
     6061 taattctttc catactgttt cggccatttc ctggcgatga gtttcgaact tatcgtcact
     6121 gtctattttg ccgctaacaa attcatgata aatatcttta gatgctttta atttagcaaa
     6181 taaataatcc atccctgact ttggaatatg gcaatcggca cattccgcac gtatcccttt
     6241 ctggttcgaa aagtggacag ttccctgata ttcctcaaaa ggtttactca tcgagtggca
     6301 ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc
     6361 gccgccacca accacaaccg ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg
     6421 catagattcc tctaaataaa ttccagaaca ccgaaatcat tgtataatta aatacacaac
     6481 gaattaaccg cttgcataac tcggcacaac actgcctgaa ataatcgata aagaatatga
     6541 tttattacaa tgtaatcat
//
LOCUS       DAJTSI010000183         6254 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000183 DAJTSI010000000
VERSION     DAJTSI010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6254
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.183"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..311
                     /locus_tag="OO327_003624"
     CDS             <1..311
                     /locus_tag="OO327_003624"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6020014.1"
                     /translation="AMIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTH
                     DLLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR
                     "
     gene            complement(387..851)
                     /locus_tag="OO327_003625"
     CDS             complement(387..851)
                     /locus_tag="OO327_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR6020015.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(848..1723)
                     /gene="nanK"
                     /locus_tag="OO327_003626"
     CDS             complement(848..1723)
                     /gene="nanK"
                     /locus_tag="OO327_003626"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR6020016.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1720..2409)
                     /locus_tag="OO327_003627"
     CDS             complement(1720..2409)
                     /locus_tag="OO327_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR6020017.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2457..3947)
                     /gene="nanT"
                     /locus_tag="OO327_003628"
     CDS             complement(2457..3947)
                     /gene="nanT"
                     /locus_tag="OO327_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR6020018.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4057..4950)
                     /gene="nanA"
                     /locus_tag="OO327_003629"
     CDS             complement(4057..4950)
                     /gene="nanA"
                     /locus_tag="OO327_003629"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR6020019.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5072..5863)
                     /gene="nanR"
                     /locus_tag="OO327_003630"
     CDS             complement(5072..5863)
                     /gene="nanR"
                     /locus_tag="OO327_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR6020020.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1585 a         1695 c         1551 g         1423 t
ORIGIN      
        1 ttgccatgat ttacattgaa cagcttgaat taatccataa aagtggtgat gtgttgtatc
       61 cagtaaaaat aaccagaaaa tcatcaggaa aaactgcctt ccaccttgtt ccattcggct
      121 taaataagac tcacgacctt ctagaggtgg aagatgcttc agaagcaatt cggctggtga
      181 ttgatgaacg tcattccatt cgctgcagta cacttacagc aactattacg aataaaaagg
      241 gcaagcgaat taagcggaca ggtatataca gtatcaaggg tgttaacata aaagaataca
      301 acgtcaggta atggcacaga gcggtccgtc agttcaggtt tggctctgtg ccaaagatgg
      361 gtcaggtcac acctgatcta ataaatttaa gccattaaat cagccttaac cttcaccacc
      421 acctttttaa tctcacccgg ctcgccaaca acgcatcccg gcttatgcgg ctctcctggc
      481 ataaacacgg cgaacattcc cggtttcaga atgatggttt gctcgttttc aatggcgcta
      541 caaagctggt aatcatcttc atggtggaac tcttcacatt ggcgcgcagt gcctgccatg
      601 ccaaacagaa tccgttcctc accgtttaat aacagctgga tatcaatgta ttgctcgtgc
      661 aattccgctt ttttctcgac gggcgattga gtgttaaacg tcatgacatt cataaaaata
      721 ttgtcgccct gtaattcgta acgacccggc gctttttctt gcggtctggc agctaaggcc
      781 agcgttaacg cgtcctgtaa cgcaggatgt aacccagcag acggtaatga ctgtacttca
      841 cccatcatca taatttttct ccctgggcca acagcgcagc cccaagtaaa cctgcatcat
      901 ggcggtaatg cgccgccagt aaatcaacat gaaatgccgc tggctcctgc gccagataca
      961 tttccaccaa cgccagatac ccttctgcca gaccaacgct gccaccaacc accacgcact
     1021 ggcaatcagt tgtggcttta atatcagcga tcagccttgc aagcgtacgt gcggagcgat
     1081 gaatcagttg ctgcgcctgc tcatcgccct gcccggcgcg tgtgaaaata gttctcgcat
     1141 ccgcgccagc cagctcgccc tgcgctgccg ccgcaatgcc gcgaccagaa gcaattgctt
     1201 ccacgcaacc tgtgcgtcca cagccgcaga ctgggccgtg tggatcggca agcgtatgcc
     1261 cgatgtgccc cgccagaccg ccagggccgg taagcagttt gccgccgctc actacaccgc
     1321 cgccgacgcc agtggaaacg gtgataaaga ccatatcggt tatatcgcct tccagcgcct
     1381 gatactctgc ccatgctgcg gcctgcgcgt cgttaatggc aatggtcggc aaattggtaa
     1441 gttgttccag cgttttgact aacggaaagt gtagcaatcc accgagatta tgcggattca
     1501 gcgccagcaa actgccgtcg cgaataattc cggttgaagc gatggcaacc cgctgcgcat
     1561 gagcttgcaa cggagagact aatgcggata aggcatcacg caaggcttct ggtgtctggc
     1621 tggctggcgt aggaagttca cgacgatcgc ggatctgccc gtcagcgcca atcagcgcgg
     1681 cggcaagttt agtaccgccg atatcaatcg ccagtatggt catagcaccg cctttttcat
     1741 cgctgtgttg taccactgac aaatgtgttc aagacgcgta atggcagaac cgaccgtcac
     1801 cgcccacgcg ccgtggcgca tcgcatctgc cgcctgagca ggcgtgttgt aacgcccttc
     1861 ggcaatcacc cgacatccgg cttcgctcaa cgttttcacc agcgccagat ccggctcttc
     1921 tggcgtttca ggcgtggtat agccagaaag cgtagtgcca ataatttcgg ctcccagctt
     1981 ttggcatgcc aggccgtctt ccggcgttga gcagtcggtc atcgccagta aaccgtgatg
     2041 atggatacgt gccagcagcg tttcaacagg caccggacgc gggcggtcgg tgccgtcaat
     2101 ggcgataatg tccgcgcccg cctgcgccag cgcatcgaca tcttcaatat aggccgtgat
     2161 gcgtaccgga gaatcctcca gatcgcgttt cacaattcca ataatcggca cgctcaccac
     2221 cgcacgcgtg gcttgcagat ttgccacacc ttcgatgcga atggcaaccg cgcccgcctg
     2281 ctctgccgct aatgccatgg ctgcgacgat ttcgggttta tcgagcgggc tgtcaggaac
     2341 cggctggcag gagacaatca ggccaccgtt agcagcgatt ttttgatcca gttgtgcaag
     2401 taacgacata catcttccct tagcgaaagg cccggtacat agaccgggca acaggattaa
     2461 cttttggttt tgactaaatc gtttttggcg ctgccaaatg gcacggcacc gctaaatggt
     2521 ttgccgtcga tagcgtcatg agtacgcaat gcttccgggc gcagccaacg ctgaacgcgg
     2581 gaaggcatat ccagaccaat cagcaggatc accacaaatg tcaggctgaa cgagagcgat
     2641 gccagcgcag tacccagatc cagacgttga gcgatcaacg cgccgatgat tggggccagt
     2701 gcgccaccca atgcgccaac gttgtaggta aagcccaggc ccgctgcacg ctggtcggta
     2761 tcgaaataac cgccaatcag tttcggtaag atcccggcga tcccttgtcc aagcatttgc
     2821 tggaagaaca gtaacagacc gagcacccag acgtttgcgc cgccaatcgc aaataccgga
     2881 ataatcagca gctgcgaggc cagcaggcta caaacgtacg ctttgcgggt tcccagccag
     2941 tcaccgagga agccacctac gcagcatccc acggccgcgc caaagccact aaagaacagc
     3001 acattggcga cagtatgcgg gttataagcc agatcggttt tcagatacgt tggcagcagc
     3061 gcctgaatcg gccatgagta gaggaaagca aacaacacga ccaccatcag cattacgccc
     3121 gttggccagc gtttacccgt actttgcacc ataaagctga taaagattgc ggcgcataac
     3181 agcccgagaa cagcgacgat tgcagcattt tgcaggttac cggcgaagca gaaccacagc
     3241 gcagtagccg ccgccagtgt cattacgata ttggcaatgc gatgttcacc acggtagaga
     3301 atatccacca ttgtgcgtac tggtgcttta cctgcgtgtt tctctttcca gtcttccgct
     3361 tccgggatgt ttttacgcag ccagagagca aagatgattg gcaaaatgcc gataaagaac
     3421 agcgcacgcc aaccccagac cggaaccacc aggctataga cctgagcggc aacgacggcc
     3481 cccacagaga agcctgaaat caaaaaacca ctggctttgt tacgcagatg ttttggccag
     3541 ctttcaatga cataggtggc gctggaaccg tattcacccg ccatccccat gccgatgacc
     3601 agacgagcga taaacatggt gatgtagcct ggcgcaaagc cgcaggccag cgtcccggcc
     3661 gagaagagaa cgatgctggt gaccattgcc agacgacgcc cgtagcggtc acccatagcg
     3721 ccgagcatca ggccgccgaa ccagcgagag ataaaggctg cggagatcag acttgccgcc
     3781 tgcaccgtcg tcagcccgaa ttcaccctgt acttcggtga gtaccagggc gattaaaacg
     3841 aaatcaaaac cgtcaagcag atatcccaac caggcagcgg aaaatgcgcg ccattgtgca
     3901 cggttgagat ggcgatacca cgggatattc tgggttgtag tactcattgt gagtctcccg
     3961 cggtgggcga tgcccacacg ctttggtatg aaaattgtag ggtacagatg cgtttatttc
     4021 ccctcacccg gtaggggcga gcgaggggaa aacaactcac ccgcgctctt gcatcaactg
     4081 ctgggccagc gccttcagtt ctggcagata tttttcatct accggtccaa acggtttgcg
     4141 gcacagcggc acagaaacga catccatata atgcaggaca gttttcaggc cgcggaatac
     4201 gcccgttttg atcagtaaat caatgacttt gttgcactca gtttgcagtt tctgcgcggt
     4261 ctggatatcg ccttctttca gcgccttcac gatcccctga tatcgccagc ccatgatgtt
     4321 gtaagtactg ccgataccac catcagcgcc tgccagcaag ccggaagcga agatttcgtc
     4381 gtaaccgtta tagagcacca gatcaggatg ttcacgacgg atctgttcca tctgatagag
     4441 atcgccagaa gtctgtttca gcgcaccgac gccaggcaac gtaaccaggg tgttgatctg
     4501 atccagggtc agttttaccc cactcagggc tggaatgttg tacaccacca tcggcaaacc
     4561 atccgctgaa tcaataattg cccgatagtg atcgcagtgt tcttcaaagc tgaaaggata
     4621 gtagaacggc gtgacggcgg agacggcatc gaagccataa cgtttagccg atgccgcaag
     4681 ttgttggctt tcggcggtgc tgacgcaacc gacgtgggcg atgagtttaa tcttaccttt
     4741 cgcctcttcg gcgacgattt ccagtacctg ttcacgctcg gaaaggcttt gtacaaaggc
     4801 ttcgccggtc gaaccaccca cgtataaacc gtcgatgccc tgctgaatat tgaactgaac
     4861 caggcggcgc agactcgctt tatccagtgc ttgttgttga tcaaaaggag tcaggagtgc
     4921 agccattacg ccacgtaaat tcgttgccat aaatacctct gaagtgatgc ttgtctgata
     4981 aacgatatac ctttatacct gttataccag atcaattaag caacacccca tacagaaagc
     5041 ttataatgcg atctgcttca ctaaagtggc attatttctt tttgttggtg gtctgaccga
     5101 aagcgtgcca ggtagcagag acgctgttga gatgcgattg caacgcacga tcggcttcgt
     5161 caggatcatg acggcggatc gcatcaacga tcgcaatatg ctgttgataa ctaacgttgt
     5221 tatgttcgtg cagtgcctga tcggtaaccg ttgggcgtgc ggcaataagc cagtcgagca
     5281 gggcaacgtg gatcgccatg aagattgggt taccggggat ctccgccagc acgcggtgga
     5341 aatcaacgtc tgaacgaatg aatgccgcgt tgttatccag cgactgactg ttgatttcca
     5401 gtgcttttgc cagcaaatcg atttgctcat cggtggcgtg ttcagccgca tagcgcacca
     5461 ggctggattc aaagaacaga cgtaattgtt cgaaatgggc aatcccacca ggatgagaaa
     5521 ggaaatcttt cgccatgccg gaaagctcgc cgatgatagt gtccgcagaa ggtcgagaga
     5581 cacgagcgcg ttcgccgttg tttatttgca ccagaccttt gcgttttaac gctgccagcg
     5641 cttcacgcac agaaggacgc ccgacgttaa agaacgccat cagttcgcgt tcagacggta
     5701 attgttcacc ttcgccaaat tcacgacggc ggatcatctg ttccagctct tcttccacca
     5761 tttcggagag ttttttacgc gccagcgggc ggctacgcaa gttgcgacca attgcaggtg
     5821 aagaatcttc ggtttgcgaa tcaaatgcgt tcataaggcc cattctgtaa ggtcagtgtg
     5881 attaacatca tcagtgacat cctatcacag gattgaaagt aggggaaaat ggcaggtttt
     5941 tctctttgtg cctcatcatt accataatta acggaataat taactattgc gaaaaattaa
     6001 tgtaacgcag ataaacacat cccgattgaa ttattcataa gactattcac aagcattatg
     6061 aatattatga atgtgttctt acaaaataat cataatcgca tattttttaa tgaaaaatca
     6121 cctcacctac aattaaaaac acgacatccg caccataaat agacttgcaa aaaatataac
     6181 atcattgttt tcaatctgcc atttatggga ttgaccgttt tcttttgaca cggagttcaa
     6241 caatgggtaa tgct
//
LOCUS       DAJTSI010000184         6239 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000184 DAJTSI010000000
VERSION     DAJTSI010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6239
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.184"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1464
                     /locus_tag="OO327_003631"
     CDS             <1..1464
                     /locus_tag="OO327_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HCR6020021.1"
                     /translation="LQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQ
                     IVTIHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERL
                     AEDLRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGL
                     RTCIEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQ
                     RGHGIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVD
                     PNWVQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPI
                     SEPLIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNW
                     LGEDSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFL
                     RGVDKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            1464..3290
                     /gene="recD"
                     /locus_tag="OO327_003632"
     CDS             1464..3290
                     /gene="recD"
                     /locus_tag="OO327_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR6020022.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(3352..4683)
                     /gene="argA"
                     /locus_tag="OO327_003633"
     CDS             complement(3352..4683)
                     /gene="argA"
                     /locus_tag="OO327_003633"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR6020023.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            4915..6168
                     /gene="amiC"
                     /locus_tag="OO327_003634"
     CDS             4915..6168
                     /gene="amiC"
                     /locus_tag="OO327_003634"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR6020024.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
BASE COUNT         1457 a         1589 c         1729 g         1464 t
ORIGIN      
        1 cttcaagaag ccggaacgca gctggaaagt gaacatgcgc tggtacgctg gttatcgcaa
       61 catatcctcg agccagacag taatgcctcc agccaacaaa tgcgcctcga aagtgataaa
      121 catctggtgc agattgtcac gatccataaa tcgaaagggc tggaatatcc gctggtctgg
      181 ttgccattta ttaccaattt ccgcgtccag gatcaggcgt tttatcacga tcgccactcg
      241 tttgaggcag ttttggatct taatgctgcg ccagaaagcg tcgacctcgc ggaggtcgaa
      301 cgtctggcgg aagatctgcg tttgctttac gtggcgctga cgcgttcggt ttggcattgc
      361 agtctcggcg ttgcaccgct ggtgcgccgt cgtggcgata aaaaaggtga caccgacgtc
      421 caccaaagtg cgctcgggcg tttgctgcaa aaaggggaac cgcaagatgc ggcagggctt
      481 cgcacctgta ttgaagcgtt atgcgatgat gatattgcct ggcaaacggc acaaactggt
      541 gataaccagc cctggcaggt taatgatgct ttaactgcag aactgaatgc gaggacacta
      601 caacgattgc ccggcgataa ctggcgtgtc accagctact ccggcttgca gcagcgtggt
      661 cacggtatcg ctcaggatct gatgccacgg ctggatgttg atgccgcagg cgtggtcagc
      721 gtcgttgaag aaccgacgtt aacaccgcat cagttcccgc gcggtgcgtc accggggaca
      781 ttcttgcaca gtttgtttga agacctcgat tttacccagc cggttgaccc gaactgggta
      841 caggaaaaac tggaactcgg cggctttgaa tcgcagtggg aaccggtgtt gaccgagtgg
      901 atcacggctg tcctccaggc acctctcaat gaaacgggtg ttagcctgag tcagctttcc
      961 gaccgcgata aacaggtgga gatggagttt tacctgccga ttagcgaacc gctcatcgcc
     1021 agccagcttg atgcactaat tcgccagttt gacccgctat ccgctggctg cccgccgctg
     1081 gagttcatgc aggtacgcgg catgttaaaa ggctttatcg acctggtatt ccgccacgaa
     1141 gggcgttatt acctgctcga ctataaatcc aactggctgg gtgaagacag ttcggcttac
     1201 acccaacagg ctatggcagc agcgatgcag gcacaccgct atgatctgca atatcagctt
     1261 tataccctgg cgctgcatcg ttatctgcgc catcgcattg ctgattacga ctatgatctt
     1321 cactttggcg gcgttatcta tctgttcctg cgtggcgttg ataaagaaca tccgcaacaa
     1381 gggatttaca caacccgacc caacgccggg ttgattgccc tgatggatga gatgtttgcc
     1441 ggtatgaccc tggaggaggc gtaatgaaat tgcaaaagca attactggaa gctgtggagc
     1501 acaaacagct acgcccgctg gatgtgcaat ttgccctgac cgtggcggga gatgaacatc
     1561 ctgccgtcac cctcgcggcg gcactgttaa gtcatgatgc cggagaggga cacgtctgtt
     1621 tgccgctttc acgactggaa aataacgagg cgtcgaatcc gctgttggcg acctgtgtca
     1681 gtgaaatcgg tgagctacaa aattgggaag aatgcttgct ggcttctcaa gcggtcagca
     1741 ggggagatga acccacgccg atgatcctct gtggcgatcg tctttatttg aatcgcatgt
     1801 ggtgtaacga gcggacggta gcgcgcttct tcaatgaggt gaatcatacc attgaggttg
     1861 atgaagctct actggcgcaa accctggaca aactttttcc agtaagcgat gaaattaact
     1921 ggcaaaaagt tgcagcggca gtggcgctga cgcggcggat ctcggtgatt tccggcggcc
     1981 caggcaccgg taaaacgacc accgtggcga agttgctggc agcgttaatt caaatggccg
     2041 acggcgaacg ctgccgtatc cgtctggctg caccaacggg taaagctgcc gcgcgcttaa
     2101 ccgaatctct cggcaaggct ttgcgacagt taccgctgac cgatgaacaa aagaaacgca
     2161 ttccggaaga tgccagcact ttgcaccgat tgctgggcgc gcagccgggt agccagcgtt
     2221 tacgtcatca tgccggtaac ccgctgcatc ttgatgtgct ggtggtagat gaagcgtcaa
     2281 tgatcgatct gcctatgatg tcgagactga tcgacgcctt gccagatcat gcgcgagtga
     2341 tctttctcgg cgatcgtgat caactggcct cggttgaggc tggggctgtg ctgggcgata
     2401 tctgcgctta tgccaacgcg ggctttaccg ccgagcgtgc caggcagcta agccgcctga
     2461 cggggactca cgttccggca ggaaccggca cagaagcggc atctttgcgc gacagtctct
     2521 gcctgctgca aaaaagctat cgtttcggca gcgattctgg cattggtcag ttagctgcgg
     2581 cgatcaaccg tggtgataaa acggcagtga aaaccgtttt tcagcaggat tttactgata
     2641 tcgaaaaacg acttttacag agcggcgaag attatattgc gatgcttgag gaagctcttg
     2701 cgggttacgg acgttatctg gatctgctgc aagcgcgtgc cgagccggat ttaatcattc
     2761 aggcgttcaa tgagtaccag cttttgtgcg ccctgcggga agggccgttt ggcgtggctg
     2821 gactgaatga gcgaattgaa cagtttatgc aacagaagcg caaaattcat cgtcatccgc
     2881 actctcgttg gtacgaaggc cgaccggtga tgattgcccg taatgacagc gcgcttgggt
     2941 tgtttaatgg cgatatcggt attgcgctgg atcgcgggca ggggacgcgc gtctggtttg
     3001 cgatgccgga cggcaatatt aagtctgtgc aaccgagtcg cttgccagag cacgaaacta
     3061 cgtgggcgat gacggtacat aaatcgcagg gatcggagtt cgaccatgcg gcgttgattt
     3121 taccgagcca acgcacgccg gtagtaacgc gagagctggt atacaccgcg gtgacccgcg
     3181 cgcgtcgtcg tctgtcgctg tatgccgatg agcgtatatt aagtgcggca atcgccactc
     3241 gtactgagcg gcgcagtggt ctggcggcgt tgtttagttc gcgggaataa acgtaattgc
     3301 cggatgcgac gcacgagtgt tacgcctgcc gcatccggtg attttcatcg cttaccctaa
     3361 atccgccatc aacactttgg atttacgctg gtagttgtac aactgctttt tgctctcggg
     3421 cagtaaatca atatccaccg gggtaaatcc acgttcctgg aaccagtgaa tactgcgcgt
     3481 ggtcagcaca aacaatttgc ttaagccgct ctgcttcgcc tgagcggcaa tgcgttccag
     3541 cagaacctcg ccccgtgatg aactgcggta atccgggtga actgccacac aggccatttc
     3601 cccaatcttc tcttccggga acggatagag cgcggcgcag gcaatagtcg tgttatcgcg
     3661 ctgaataatg gtgaatttgt cgatttccat ctccagctgc tcgcgagaac ggcgcaccag
     3721 aataccttgc tgctccagcg ggcgaatcaa ctccagaata ccgccaatat cgttgattgt
     3781 tgcgcgacga atctgctcgg cgctttccat cacaatctgc gtaccgatac cgtcgcgtga
     3841 gaacaactct tgcaacagcg cgccatcttc ctgataactg attaaatgac agcggcgcac
     3901 gccgctgcgg caggctttca ctgcgccacg caaaaagcgc accgtaccgg agttgtaatc
     3961 gcctttctct tcctgggctt ctacccgtgc ttgcgcttcg ttagggaaaa gttcggagac
     4021 aatatcaccg tcgtcattag taacgccctg ggaagagcaa aaaccaatca tcttttcagc
     4081 tttcagtttg atggctagtt gagtggcaat ctcttccgag gtcagattaa agctctcgcc
     4141 agtgactgaa acagcgaccg gccccattag cactattgca ccgctttcca gttgacgatg
     4201 gagcgcgtct tcatcaatcc gccggatacg cccgctatgg cagtaatcca cgccgtcatc
     4261 gacgcccagc ggctgggcaa taataaaatt gccactaacg acgttgatat gcgcgccctg
     4321 cagcggcgtg ttattgagac tcattgacag gcgagcagta atatccagtt gcaatgttcc
     4381 cgcagcctgc ttcaccagtt ccagtgtttt ggcgtcggtc acacgtatat tcttgtgata
     4441 cagcggttcg tggtgatgcg cagccagatt tgcgtcgatc tgcggacgtg cgccatagac
     4501 caccaccaga cggatgccga ggctgtgcaa caacccgata tcattaacga tactggagaa
     4561 attctcatgc tcaatggctt caccgccgag catgatgaca aacgtttttc cccggtgggt
     4621 attgatatag ggaaccgaat ggcggaatcc ctcgaccagc tcggttttac gttcctttac
     4681 cacggcacac ccctttgcat gattatttga aattattgta tttttattct gttttttcgc
     4741 agggtgcaag tgtaaatttt atgcgagagc gactttttta tcagtaatgc cgtgaatata
     4801 aaaagaatgt ttaccgttta ttagatgaca gattatgcgt ctttcgctaa agtttccggt
     4861 caaattagtc gtttacttgt tacacagctt agatttcttt tgctcgggag aggcatgtca
     4921 ggatccaaca ctgcaatcag ccgtcgtcgt ttactgcaag gcgcgggcgc tatgtggcta
     4981 ttgagcgtaa gtcaggtcag cctggctgcg gttagccagg tcgtggcggt gcgcgtctgg
     5041 cctgcgtcca gctacacccg cgtgacggta gaatcaaatc gtcagctgaa atataagcag
     5101 ttcgcgttga gtaaccctga acgcgtggtg gtggatatcg aagatgtaaa cctgaactcg
     5161 gtactcaagg ggatggcggc acaaattcgt gcagacgacc cgttcatcaa gtcggcgcgc
     5221 gtcgggcaat ttgacccgca aaccgtacgc atggtttttg aattaaagca aaacgtaaaa
     5281 ccgcagctgt ttgcccttgc gccggtcgcc gggtttaaag agcgtctggt gatggacctc
     5341 tatccggcca atgcacagga tatgcaggac ccgctgctgg cgctgctgga ggattacaac
     5401 aaaggcgacc tcgaaaagca ggtgccgcca gcgcaaagtg gtccacaacc gggtaaagca
     5461 gggcgcgatc gtccgattgt cattatgctt gaccctggcc acggtggcga agactccggt
     5521 gcggtgggga aatacaaaac gcgcgaaaaa gatgtggtat tgcaaatagc tcgccgtttg
     5581 cgctctctga tcgaggaaga gggcaatatg aaggtgtaca tgacgcgcaa tgaagacatc
     5641 ttcattccgt tgcaagtgcg cgtagcaaaa gcccagaaac agcgtgctga cctgtttgtc
     5701 tctatccatg ccgacgcctt taccagtcgt cagccgagcg gttcctcggt gtttgcgctc
     5761 tcaaccaaag gcgcaaccag tactgcggca aaatatctgg cacaaaccca gaacgcctcg
     5821 gacttgattg gtggcgtgag caaaagcggt gaccgctatg tcgaccacac catgttcgat
     5881 atggtgcagt cgctgactat tgctgacagc cttaagtttg gtaaagcggt actgaataag
     5941 ctcggaaaaa tcaacaagct gcataaaaat caagttgaac aggccgggtt tgccgtacta
     6001 aaggcaccag atattccctc cattctggtc gaaacggcgt ttatcagtaa cgttgaggaa
     6061 gagcgtaaac tgaaaacggc gactttccag caggaagttg cggagtctat tcttgcgggg
     6121 attaaagcgt attttgccga tggggcaacg ctggcgagaa ggggatgata aaaaggcgct
     6181 gaatggcgct ttttttatgg gagatcagaa acaaaaaaac acccgttagg gtgttcaat
//
LOCUS       DAJTSI010000185         6200 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000185 DAJTSI010000000
VERSION     DAJTSI010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.185"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO327_003635"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO327_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR6020025.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2089..2238
                     /locus_tag="OO327_003636"
     CDS             2089..2238
                     /locus_tag="OO327_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020026.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(2983..3423)
                     /locus_tag="OO327_003637"
     CDS             complement(2983..3423)
                     /locus_tag="OO327_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR6020027.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3641..4030
                     /locus_tag="OO327_003638"
     CDS             3641..4030
                     /locus_tag="OO327_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020028.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4074..4478
                     /locus_tag="OO327_003639"
                     /pseudo
     CDS             4074..4478
                     /locus_tag="OO327_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4585..5421)
                     /locus_tag="OO327_003640"
     CDS             complement(4585..5421)
                     /locus_tag="OO327_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR6020029.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(5421..>6200)
                     /locus_tag="OO327_003641"
                     /note="aph(3'')-Ib"
     CDS             complement(5421..>6200)
                     /locus_tag="OO327_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR6020030.1"
                     /translation="GESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGER
                     DRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLG
                     AVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELP
                     VRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEE
                     NWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
BASE COUNT         1434 a         1617 c         1640 g         1509 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aaaaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgttgcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattccattt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt atacatcact tttacatcat tccttcagct catacgtcac tttcatcctc
     2641 tgtgccattt gtacatcacc cccttcctga acgtgctgac agcgggtttt ccggctgcgc
     2701 cggcaacgct tcacggccac gcaataaatt gcgtccccgt tcaccgtttt cggatgggaa
     2761 gaacgccgga aacaggactt accccggctg aatggtgtga atttccgcgc tatgcacttg
     2821 tgcgcatact catgcatgcc gtaaaaacag agcctgcgcg tttctggcgg gttttcgggt
     2881 ggtttgttgc ctgttttacc ggtttcccgt cagaaacgcc ctgagggcct ctcaggcggt
     2941 gctgataaca gatgttatgg taaatcagag tcacctgcac tgttactctg aaccggagcg
     3001 aaacagccgc gaccagaatc cccgcttttt ttcctgtgcc agcgcgtcag ccagacggct
     3061 aatttcggcc tgtagttgtt ccctctcctc atttcgcagg cggttttctg cttcccgcgc
     3121 catcttgtct tcaagcatta gggtaacagt ctgctgcaga gcgtccagtt tctgctgaat
     3181 gacgtctagc tgagcatttt ccgcctggct aacgacagat ttggagtgtc cctccttgtg
     3241 cctttttggt gtctcagaag tgcgcagctc gccataggcc cgaatcagtt cactggtctc
     3301 gaactcccgg cgatcatttt ttcctgtgcg atagctgaca aggcctttag ccatgtcacg
     3361 atataattgc gagcgcgact ttcctgttaa tttcagtgtc tcttgaactg tgtgccatgc
     3421 cataaaagtg acagtgtctc ctgtgtgtac cgtattgtaa cagcagaaca tattactgtt
     3481 ttaacacaaa aggtctgagg ctgcccgtga ggcgtgaaaa atacacaaaa aacctgttga
     3541 cggtgtggtt ttgacgggcg tagtatttcg aatactgtat ataaacacat tgttataaat
     3601 acagtttttg ctgtttttaa aagtcaaaga ggataatttt atgagtacag agttaaatag
     3661 cggtactgtc ggtggttttg gtcacatggg accaaatgac ccctacaatg atggtcatag
     3721 cggctctgga agttcgggag gtagttttaa cggtggtggt aatggtggcc atgataatag
     3781 tagtggttca tcttatactg catcctttgg tctcatcact ggccgacacc cgataactgt
     3841 cccaggtacg aagaatatta attctgtcga tctttctgct gtcattaata acattataca
     3901 tcatgaaggt gcacgcaata acatgtacct agatactgcg aacaatgtaa gagtgggagt
     3961 gacgggcact ggctggcaat gtctagcaac ggcaggcatt tcggctgagg gtaaaagaac
     4021 tttccgctaa gcgatagact gtatgtaaac acagtattgc aaggacgcgg aacatgcctc
     4081 atgtggcggc caggacggcc agccgggatc gggatactgg tcgttaccag agccaccgac
     4141 ccgagcaaac ccttctctat cagatcgttg acgagtatta cccggcattc gctgcgctta
     4201 tggcagagca gggaaaggaa ttgccgggct atgtgcaacg ggaatttgaa gaatttctcc
     4261 aatgcgggcg gctggagcat ggctttctac gggttcgctg cgagtcttgc cacgccgagc
     4321 acctggtcgc tttcagctgt aagcgtcgcg gtttctgccc gagctgtggg gcgcggcgga
     4381 tggccgaaag tgccgccttg ctggttgatg aagtactgcc tgaacaaccc atgcgtcagt
     4441 gggtgttgag cttcccgttt cagctgcgtt tcctgtttgg ggtcgtttgc gggaaggggc
     4501 ggaatcctac gctaaggctt tggccagcga tattctccgg tgagattgat gtgttcccag
     4561 gggataggag aagtcgcttg atatctagta tgacgtctgt cgcacctgct tgatcgcggc
     4621 cgcgatagct agatcgcgtt gctcctcttc tccatccgca ttccaagctg cggaaaggca
     4681 cccataagcg tacgcctggt cgagcaggcg acgcggatcg acgtccagcg cacgagagaa
     4741 tgcgtccgcc atctgtgcaa tgcgtctagg atcgagacaa aggtcgtctc tgtcagccgg
     4801 atcgtagaac atattggcgg cgccaaagcc cacttcaccg accagaccga cgggatctat
     4861 caccagccag ccgcgactgg agaacatgat gttttcatga tgcagatcgc catgtagccc
     4921 acgcagttcc gaggcattgc tcatcatttg atcggctata atcgccgcgt ggacgtagtc
     4981 agtttgacaa cctgcgtttt gatcatcgcg cgcccgctga aacaaagctg caaagcgatc
     5041 ccggatcggg agaagggcag aaggcagggg ttcctcagat gcggcataca gcttcgccat
     5101 tagttccgct gcaatttcgg tcgcctggta gtcgccgtgc tcggcaacga tgtgagagag
     5161 cattcgctcc ccggcatatt cgagcaacat cagattgttc tcacgaccga gcaaccggac
     5221 tgctcccctc ccattgcgcc ataccagata gtcggccccg cgcagttcat cagcaatgtc
     5281 ttctataggt ttcaatccct tgacgattgc aggagtcccg tctggcaatg aaactttcca
     5341 aacgaggctg gaaaaggtgt ccgcaatgag aacaggttgc gaaacgtgcc aatgagcagg
     5401 aaaaacaggc ggcatgaaca tcaaccccaa gtcagagggt ccaatcgcag atagaaggca
     5461 aggcgttcgc ggtcgggggc ttcgatcccc aatacattga ataggacagc gaaggcgcgc
     5521 tctgcttcat ctggcgctgc ccagttctct tcggcgttag caatcatgag tgccaaatcg
     5581 gcatagcgat ctgctgttcc gagccgccca aggtcgatca gacccgtgca ttgaagagtt
     5641 ttagggtcca ccatgaagtt cggcatgcag ggatcaccat ggcaaacaac catatcggtg
     5701 cgctcttggt cgagccgcac cggtagctct cgttcgacac gagccaaaag atcgagctgc
     5761 ggcgtactct tgtcctcgtc cggtaagaag tcgggattga cggcattgcg ggacaccaca
     5821 tcaacggcgc gtccgaacat tcgcgacagc ctgcgctcaa acggacattg atcaaccgat
     5881 aggctgtgaa cagcgccaag ttgctgcccc attgacggcc acgctttgag caaatccgct
     5941 ccagacagat cagccgccgg tactcccgga attgccgtta tcaccaagca tgcaccctcc
     6001 tgttcctcct gccagttgat gacctcgggg caagccacac ctcgaccttt gagccaaatg
     6061 aggcggtcac gctctccagc gagctcaccg cggcgggaag caggtgcgat tttcgcgaag
     6121 gcatgcccgt caccacgtcg aaaaacaaaa tcaccagatt ctccgcctct gacaggcaac
     6181 cagtcagaat gcgattcacc
//
LOCUS       DAJTSI010000186         6136 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000186 DAJTSI010000000
VERSION     DAJTSI010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.186"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="OO327_003642"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="OO327_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR6020031.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="OO327_003643"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="OO327_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR6020032.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="OO327_003644"
                     /pseudo
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="OO327_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(5797..6054)
                     /locus_tag="OO327_003645"
                     /pseudo
     CDS             complement(5797..6054)
                     /locus_tag="OO327_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1618 a         1442 c         1450 g         1626 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt acaggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt ttaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgccta ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgtggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagataaat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcaacagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg ccccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacgacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atataaagcg
     6061 cgcaaaatta ttcaagatat ttttgaaaat cattcaagat aggctatcgc gtgtgatagc
     6121 aaaaacattc aagata
//
LOCUS       DAJTSI010000187         6058 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000187 DAJTSI010000000
VERSION     DAJTSI010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.188"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..143)
                     /locus_tag="OO327_003646"
     CDS             complement(<1..143)
                     /locus_tag="OO327_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HCR6020033.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQ"
     gene            379..651
                     /locus_tag="OO327_003647"
     CDS             379..651
                     /locus_tag="OO327_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR6020034.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(617..1024)
                     /gene="nudG"
                     /locus_tag="OO327_003648"
     CDS             complement(617..1024)
                     /gene="nudG"
                     /locus_tag="OO327_003648"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR6020035.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            1111..1731
                     /locus_tag="OO327_003649"
     CDS             1111..1731
                     /locus_tag="OO327_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR6020036.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1740..3047)
                     /gene="ynjE"
                     /locus_tag="OO327_003650"
                     /pseudo
     CDS             complement(1740..3047)
                     /gene="ynjE"
                     /locus_tag="OO327_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(3114..3767)
                     /locus_tag="OO327_003651"
     CDS             complement(3114..3767)
                     /locus_tag="OO327_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6020037.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3767..5238)
                     /locus_tag="OO327_003652"
                     /pseudo
     CDS             complement(3767..5238)
                     /locus_tag="OO327_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(5211..6058)
                     /locus_tag="OO327_003653"
                     /pseudo
     CDS             complement(5211..6058)
                     /locus_tag="OO327_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1427 a         1621 c         1599 g         1411 t
ORIGIN      
        1 atctggcgat attttggatt ttgttcaaga aaaggccaga gtgtggtcat tacttcacga
       61 acggcttgcg cgaactcggt ttgattcggg tcgcgctttt ggacatggtt gaggaatgac
      121 tccagagaat atgtctgatc catagatata aaaaccttat atattaatac gattgtgctt
      181 atgttgttta tgtcatgtgc ttttgcagtt ttcgactata ccatcaagaa tgtgacggta
      241 agcaagaaaa aaatgaccca ggaaagcaaa ttaataacga gagtaatctc ataaccaaat
      301 gcgataactc agacgctaaa gttttgccac ccggctaccg ttatcagtag cagagacaat
      361 aacgttaagg agttccctgt gagtcgagca ttgttcgccg tagtattagc atttccatta
      421 attgcactgg ctaacccgca ttatcgtccg gacgtggagg taaacgtacc gccagaagtg
      481 ttcagttcag gcgggcagtc agcgcagccc tgtactcagt gctgcgttta tcaggatcaa
      541 aattattctg aaggggcggt aattaaagcc gaaggaatac tgctgcaatg ccagcgtgat
      601 gacaaaaccc ttagcactaa tccgctgctc tggcggcgcg taaagccata aacgcctcta
      661 ataatggaat gtcagcaggg gccagcggat attgcagcgc ctcttcaggt gagcaccaga
      721 ccagcgcctg atgttcatgt gcctgtaacg tcccgtggaa gtcgggtacg tgccaggcat
      781 gaagatggat aatccggccc gaaacttctc gctgatggct ggcaacatac tcacccacag
      841 ttgcttcgat gtccaattct tcgtttaact cacgcaccag cgcctgctgc tggctttcat
      901 ccagctcgac tttacctccg gcaaactccc ataatcccgc ctgatcgctc tgggcggggc
      961 gttgcgcgag taaaattttg ccatcacgtt caatgatggc ggcaacaact tcaatcattt
     1021 tcataaatta cacacgggca gataagcgaa aaatgccata atatccgcac tgttgctcaa
     1081 tcagaaaggc tttatattta aggagtgcct gtgctagacc gccatcttca tccccggatt
     1141 aaaccgttgt tgcatcagtg cgtgcgggtt cttgataaac cgggcattac gccagatggt
     1201 ttaacattag tgggatttgc catcggcgtg ctggctctgc cgtttctggc gctgggctgg
     1261 tatctggcgg cgctagtcgt cattttgttg aacaggctgc ttgatggtct ggacggtgcg
     1321 ctggcgcggc gcagagggct taccgacgcg ggcggctttc ttgatatttc tctcgatttt
     1381 ctcttttacg cgctggtgcc gtttggcttt attcttgctg caccggagca aaatgcgctg
     1441 gcaggcggct ggttgttgtt tgcgtttatc ggcacgggca gcagttttct cgcctttgct
     1501 gcactggcag caaaacatca gattgataac cccggttatg cacataagtc gttttactat
     1561 ctggggggat taaccgaagg cacagaaacg atcttactgt ttgtgctggg atgtttattc
     1621 cctgcatggt ttgcgtggtt tgcgtggatc ttcggtgcgt tgtgctggat gacgaccttt
     1681 acgcgggtgt ggagtgggta tctgacgctg aagtcgctcc agcgtcagta attgcaatgt
     1741 tatttgctac tgcccgggcc gcgttcaccg gttgcaaccg gattttttgg atcgctgctc
     1801 cattcgtacc agccgccgtc atacacggaa acattcttcc aacccatggc gcgtgcgtac
     1861 ataaaggttt cggacgcgcg ccagccggtg ccgcagtaga atgaaacttg ctgatctggt
     1921 ttgatattcc atgctttcca catagcggta atatcatcgg cgctacgcat ggtgccatcc
     1981 gggttataga aatcttccat atgcgtcgag tcgctaccag cgtgtcccca acgtgctccg
     2041 gctatttcac cttttggttt aatatagctg taaccgctgg tcgtaccgat aaattctggc
     2101 cacgaacgaa tgctcaccag cgatgcatcc tggcgatgca gcagtccacg ggcctgttcc
     2161 atatcgagca tcaactgcgg ttgtgccggg atcttcacgc cgaaatccgg ttccgctttc
     2221 actttcggtg gcgttcctcg ctcaacaggc agtcccgcgt cggaccaggt ttgccagccg
     2281 ccatccagca gacgcacatc tttcacgcct gcgtagagca taatttgcgc cacacgcgct
     2341 ccagcgtata cgtcacgccc gtacagaatg accgtggtgt catggcgaat gccgtgtttt
     2401 gccagcatcg cttttagttg ttcatcagaa actttgttcc acagcggttc gctttccact
     2461 tcgttggtat cgatgtagtc agcgccagga atatggctga taaggtaaag cttaggagcg
     2521 ccctaggccg cttcaatgac tttccagtca ccgacaggtt tcgccgtaac ctctttacct
     2581 tgttgcaggt catgcagcca ttgcggataa accagctgct caaagtgcgg cagtttttgt
     2641 agacgggaag gttcgcttag cgcgtcactc agaatggaga tatgcgtaaa acctgctttt
     2701 tgcagtcgcg ttttgacggc gtcaacatct ttgccattac cgtacagcgc caccggggca
     2761 tcggctttca ggttatgttg cttgatccac gcgttgagct gttcggtgct cattttgtcc
     2821 agccagctag cagagaggtt taaggcggca ggttcatgac cagaagggcc atttaaggtt
     2881 tgtggccagc cgttataaaa cgcgctgggg cgagtatcta tcgctttgcc attttgttgc
     2941 tgaagttggt caagtgtaag aggcttcgcc agttcagcgg cccacgaaga agcgcaagcc
     3001 agccctaaag ccattgccag cgcggtcatt tgagaaacac gtttcatcga aaatcccgac
     3061 attgtagttt tgagtgcgcc attctgaact gacgcattga gaaaaacttt gcgttatcgc
     3121 agtttgttgt aattttctga ccactgcgcc atatccagaa cagaactatc agcaggaaca
     3181 tcctggagat cgtgcgttac ctgaacgacg gggatcgcca gggcgcgaac ttcgctgaac
     3241 acccactggc gaaaattatc gcgcagagcc acatcaagac ggctgaatgg ctcatccagg
     3301 agtaacgctt ttggctgggc gagaagggcg cgtagtagag caacgcgcgc tcgctgaccg
     3361 ccagacaaag tggcaggatc ctgatggaaa gctccgtcaa ggcctgaacg ctcaagtgca
     3421 tcattcacgg cattacgtcg ggcattcccc ttaagtgtcg ccggtagcgc cagcagtaaa
     3481 ttttgcccga cgctgaactg gtcaaataac agtgcatcct gaaaaagaat gccaatctga
     3541 cgctgtgcgg tgggtagcat gtcaatccgt tgctcattga gccatagctc acctgtacaa
     3601 gaaaactgtc cggccagtgc accaatcatc catgaaaaca gagtggattt tccacagcca
     3661 gacggcccca ttaacgtgac gatgtcacct ttatcaaccg taaagttaac gtttgtcagc
     3721 aagcggcttt ctggtaaacg tagcgaaaca tttttcacgc agagcattag cggattcctt
     3781 gtctgacata acctacccat tttgcgacta acgcggtcag ggcaaaaata ataagcggta
     3841 acaacagttg ccataacgcc tgggcggcga gaataccgtt gctgccgccg ctgcttaatg
     3901 ccaccgcctc agtggtgagc gtcggaaaac gccccgcgcc cagccacagc gtcggcatat
     3961 actgcgcaat actgactgaa aatcccaccg cgaaggcaat cagcacaggg cgcaacatga
     4021 gtgggcattt cacgtaaaag aagattttgg cccgcgacca gcccagtgtt tgcgcaatca
     4081 aaattaaccg tgaatcaatg cgctgccagg cgggttgcag gataaacagc atccacggca
     4141 ttacccacag cagatgcccc cagaccaccg ccgtccagct tccatccagg ttcagccata
     4201 gcgccagcgt gtactggccc gccaccagtg gcagagcggg caatagaatg ggcaaccata
     4261 gccacaactg gcgacgctgt gccccccatt ccagccacag cagtaacagg agcaaagcga
     4321 tgaatgttgc taccagcccc attgtcagac tgttgatgag tgcttcacta ttgatcgtcg
     4381 actgatccgc gaggatcgcc agcagaacca cacacagcac accggttaag ggtaaaaaac
     4441 tcgccagcgt agtgcctggt aataaaggtg tggcaggctt gcgaatgcca tctacgcggg
     4501 gaatagtgcg ccgccagctg cgccaaaacg taggcggcga gtaacagcat cagcagcaga
     4561 ctggcgagtg cgcctttcgt ttgttgatcc gcgtcgccct gagttaacca ctgccagcta
     4621 attactgcca gcgtcggtgg attacctggc ccaagaataa tcgccacatc cacgaccgaa
     4681 agcgaccagg caacaatcgc cagcatcgcc attgccagcg gctgtagccc agcgaatcca
     4741 gcacaatgac ctgctgcaac agccgttttt cgctcaatac tgccactaag atccacagca
     4801 gaaatgcgct ttctttcact gcaagggtga ggcccagacc gatccaaatc ggtccattgg
     4861 cggagtgaaa tacgggaaat agtcatatag cagccctccg tcagcaaaga gcagcagggc
     4921 gctggtggca aaagccacat ggggaatggc gagcagccac ggcagacggg cgcacatacg
     4981 ctgccatttc ggcccaggcc atagcgccac aatcaccagc agggcaatca acaatgcccc
     5041 gaccgccgcg atggttgtcg acaccagcgt tgccagtaat gcctgcggta actgtggatc
     5101 ggcaaataac gcctgccagc gtgtcaacga caacgcaggc gatatcagcg tgagagctgc
     5161 cgggatcagc ggtgcataaa tcaccgccac catcgcccac agcaaaaaga ttaatgcgta
     5221 ccgtaacggc gtaaccattc ttgttccagt gcattcaccc atcctgcgtg cggttcagcc
     5281 agtaccggcg gcagatcctg cggcattctt gattgcaata tttcgcgctg cccgtcaggc
     5341 agtttttgcg gatcgagaac agaaggatcg ccccagacaa cgggatctgc tttacgcagt
     5401 tgcgcatcgg gtgagagcag gaaattggca actaccttcg ccgcagcact ggcattcgcg
     5461 ttggctggaa tggtgacgaa atgcacgttg ccaatcatcc cctcgcgaaa gccaaaactg
     5521 taactgcttg ccggcaaatc gccgctggcg attttttgct gcgcatgtgc ggggttaaag
     5581 gtcagcgaca ggcgcaatgt gccggctttc agcagagcat ccatccgcgc gggcgaaggc
     5641 gggaaatcct ttccttcgcg ccacaaatac ggatgcagtg catcaagata ttgctacaag
     5701 ggcgcagtga cacgggcgaa agtcgcatcg tccggcgctt cttttaatgc ggcgggatct
     5761 ggcgtcagca taatcagcaa ctgttcaaga aacgccgtgc cggtaaagtc cggtgggcgc
     5821 ggataagtaa ccgtgccggg attggcttta gcaaactcca gtaaggcttg cggcgtttgt
     5881 ggtggctgtg gcgtaacatc gcggcgggcg ataaacgtca gttgtgcgcc gccccagggc
     5941 gattccgcac cttgtgtcgg cactgaaaaa tctccccgca ccggcagctg tgtgtcgaca
     6001 tagcgccagt tgggcagagt ctccgcccag cccgtttgta ataaattggc ctctttta
//
LOCUS       DAJTSI010000188         5986 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000188 DAJTSI010000000
VERSION     DAJTSI010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5986
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.189"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..456)
                     /locus_tag="OO327_003654"
     CDS             complement(<1..456)
                     /locus_tag="OO327_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR6020038.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAA"
     gene            699..1901
                     /gene="fes"
                     /locus_tag="OO327_003655"
     CDS             699..1901
                     /gene="fes"
                     /locus_tag="OO327_003655"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCR6020039.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            1904..2122
                     /gene="ybdZ"
                     /locus_tag="OO327_003656"
     CDS             1904..2122
                     /gene="ybdZ"
                     /locus_tag="OO327_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCR6020040.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            2119..5964
                     /gene="entF"
                     /locus_tag="OO327_003657"
     CDS             2119..5964
                     /gene="entF"
                     /locus_tag="OO327_003657"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCR6020041.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWIQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLV
                     SQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAP
                     GRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTP
                     GQVMVASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVL
                     SRYLDPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGIAARLRARGEQV
                     AFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGN
                     YADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAELDIYRQDCAHVDI
                     ISPGAFVKIGPIIRATLNR"
BASE COUNT         1307 a         1586 c         1715 g         1378 t
ORIGIN      
        1 agctgccgga ccacgcagaa cttcaatacg ttcaatcatt tcaggtggca cccaggaggt
       61 atcaccacgg gtatcgcgct cgccacgcca gccctgacgc accgagttac ggctgcttac
      121 cggcttgccg tcaatcaaaa tcagcgtgtt ttccggaccc ataccgcgaa tatcaatctg
      181 tcggttattc ccacgctgac cactggtgga gttaccggtc aggttaacgc ctggcatggt
      241 acggatgatc tccgacacat cgcgggcaac cgggtttttg cggatttcat ctgcggtgat
      301 ggtcgaaacg ccaggagcct gcaagttctg ctcggcggcg gtaacgacaa tggtatcgtc
      361 atgtgaaaca ggtgtatcgg tcggctcttg tgcctgcgct accccataaa tccccagatt
      421 gaccaacaag gccagggaat gaatcttctt gttcattgtt ttattcctgc atttttgcca
      481 cgaattgcaa ctgtcgggca tggtcgtcat caacacgacg catcccgtta ccgcgaaaac
      541 ctttgatcct gaaagacacg cagtgcagtt ggttaattaa tgtccgcgct tcccacggcg
      601 cgccattacg ctattgcaaa tgcaaatagt tattaataac attatcaata tatttctgca
      661 atcaatgaaa aattgcacag taaacatggg gttatggtgt gacggcgtta aaagtaggaa
      721 gtgagagctg gtggcagtcg aaacatggcc cggaatggca gcgtctgaat gacgaaatgt
      781 ttgaggtcac tttctggtgg cgtgatcccc aaggttctga agaatactcg acgataaagc
      841 gcgtatgggt ctacatcact ggtgtgaccg atcatcatca gaacagccag ccccagtcga
      901 tgcagcgaat tgcaggtact gatgtctggc agtggacgac acaactcaat gccaactggc
      961 gcggcagcta ctgctttatt cccaccgaac gcgatgacat tttttctgca ccatcccccg
     1021 atcgcctcga attacgtgaa ggctggcgaa aactattacc ccaggcaata gccgatccgc
     1081 tgaatccaca aagctggaaa ggcgggctag ggcacgctgt ttctgcactc gaaatgccgc
     1141 aagcgcctct gcaaccggga tgggattgtc cgcaagcgcc agaaatacca gccaaagaaa
     1201 ttatctggaa aagtgaacgg ttgaaaaatt cacgtcgtgt atggattttt accaccggcg
     1261 atgtaacagc agaagaacgc ccgctggcag ttttgctcga tggcgaattt tgggcgcaaa
     1321 gtatgcccgt ctggcctgtg ctgacttcgc tgacccatcg tcagcaactt cctcccgccg
     1381 tgtatgtgtt gatcgacgct atcgacacca cgcaccgcgc ccacgaactg ccgtgtaatg
     1441 cggatttctg gctggcagta cagcaagagt tattacccct ggtgaaagtt attgcccctt
     1501 ttagcgatcg cgccgatcgc accgtggtcg ccgggcagag ttttggtggg ctgtccgcgc
     1561 tgtatgccgg actgcactgg cctgaacgct ttggctgtgt attaagccag tcaggatcgt
     1621 actggtggcc gcatcggggc gggcagcaag agggcgtgtt acttgaaaag ctaaaagctg
     1681 gtgaagttag cgccgaaggt ctgcgcattg tgctggaagc gggtattcgc gagccgatga
     1741 tcatgcgggc caatcaggcg ctgtatgcgc aattacaccc tataaaagaa tccattttct
     1801 ggcgtcaggt tgacggcgga catgatgcgc tttgttggcg cggtggcttg atgcaggggt
     1861 taatcgacct ctggcaacca cttttccatg acaggagttg aatatggcat tcagtaatcc
     1921 cttcgatgat ccacagggag cgttttacat tttgcgcaat gcgcaggggc aattcagtct
     1981 gtggccgcaa caatgcgcct taccggcagg ctgggacatt gtgtgtcagc cgcagtcaca
     2041 agagtcctgc cagcagtggc ttgaagccca ctggcgtact ctgacaccgg cgaatttcac
     2101 ccagttgcag gaggcacaat gagccagcat ttacctttgg tcgccgcaca gcccggcatc
     2161 tggatggcag aaaaactgtc ggatttaccc tccgcctgga gcgtggcgca ttacgttgag
     2221 ttaaccggag aggttgatgc gccattactg gcccgcgcgg tggttgccgg attagcgcaa
     2281 gcagatactt tgcggatgcg ttttacggaa gataacggcg aagtctggca atgggtcgat
     2341 gatgcgctga tattcgaact gccagaaatt atcgacctgc gaaccaatat tgatccgcac
     2401 ggtactgcgc aggcattaat gctggcggat ttgcaacaag atttgcgcgt cgatagcggt
     2461 aaaccactgg tctttcatca actgatacag gtggcagata accgctggta ctggtatcag
     2521 cgttatcacc atttactggt cgatggcttc agttttccgg ccattacccg ccagatcgcc
     2581 aacatttact gcgcattgct gcgtggcgaa ccaacgcctg cttcgccgtt tacgcctttc
     2641 gctgatgtag tggaagagta ccagcaatac cgcgaaagcg aagcctggca gcgtgatgcg
     2701 gcattctggg tggaacagcg tcgccaactg ccaccgcccg cgtcactttc tccggcacct
     2761 ttagcggggc gcagcgcctc ggcagatatt ttgcgtctga aaatggaatt taccgacggg
     2821 gaattccgcc agctggctac gcaactttca ggtgtgcagc gtaccgattt agcccttgcg
     2881 ctgacggctt tgtggctggg gcgattgtgc aatcgcatgg actacgccgc cggatttatc
     2941 tttatgcgtc gactgggctc ggcggcgctg acggctaccg gacccgtgct caacgttttg
     3001 ccgctgggta ttcatattgc ggcgcaagaa acgctgccgg aactggcaac ccgactggca
     3061 acccgactgg cagcacaact gaaaaaaatg cgtcgtcatc aacgttacga tgccgaacaa
     3121 attgtccgtg acagcgggcg agcggcaggt gatgaaccgc tgtttggtcc ggtactcaat
     3181 attaaggtat ttgattacca actggatatt cctggtgttc aggcgcaaac ccataccctg
     3241 gcaaccggtc cggttaatga ccttgaactg gccctgttcc cggatgaaca cggtgatttg
     3301 agtattgaga tcctcgccaa taaacagcgt tacgatgagc caacgttaat ccagcatgct
     3361 gaacgcctga aaatgctgat tgcccagttc gccgcggatc cggcgctgtt gtgcggcgat
     3421 gtcgatatta tgctgccagg tgagtatgcg cagctggcgc agatcaacgc cactcaggtt
     3481 gagattccag aaaccacgct gagcgcgctg gtggcagaac aagcggcaaa aacaccggat
     3541 gctccggcgc tggcagatgc gcgttacctg ttcagctatc gggaaatgca cgagcaggtg
     3601 gtggcgctgg cgaatctgct gcgtgagcgc ggcgttaaac caggggacag cgtggcggtg
     3661 gcactaccgc gctcggtctt tttgaccctg gcactccatg caatagttga agctggcgcg
     3721 gcctggctac cgctggatac cggctatccg gacgatcgcc tgaaaatgat gctggaagat
     3781 gcgcgtccgt cgctgttaat taccaccgac gatcaactgc cgcgctttag cgatgttccc
     3841 aatttaacca acctttgcta taacgccccg cttacccccc agggcagtgc gccgctgcaa
     3901 ctttcacaac cgcaccacac ggcttatatc atctttacct ctggctccac cggcaggccg
     3961 aaaggggtaa tggtcgggca gacggctatc gtcaaccgcc tgttgtggat tcaaaatcat
     4021 tatccgctca caggcgaaga tgtcgttgcc caaaaaacgc cgtgcagttt tgatgtctcg
     4081 gtgtgggagt ttttctggcc gtttattgcc ggggctaaac tggtgatggc tgaaccggaa
     4141 gcgcaccgcg acccgctcgc tatgcagcaa ttctttgccg aatatggcgt aacgaccacg
     4201 cactttgtgc cgtcgatgct ggcagcattt gttgcctcgc tgactccgca aaccgctcgc
     4261 cagagttgcg tgacgttgaa acaggttttc tgtagtggtg aggccttacc ggctgattta
     4321 tgccgcgaat ggcaacagtt aaccggcgcg ccgttgcata acctgtatgg cccgacggaa
     4381 gcggcggtag atgtcagctg gtatccggct tttggcgagg aactggcaca ggtgcgcggc
     4441 agcagtgtgc cgattggtta tccggtatgg aatacgggtc tgcgtattct tgatgcgatg
     4501 atgcatccgg tgccgccggg tgtggcgggt gatctctatc tcaccggtat tcaactggcg
     4561 caggggtatc ttggacgacc cgatctgacc gccagccgct ttattgccga tccttttgct
     4621 cctggtgaac ggatgtatcg taccggagac gttgcccgct ggctggataa cggcgcggtg
     4681 gagtacctcg ggcgcagtga cgatcagcta aaaattcgcg ggcagcgtat cgaactgggc
     4741 gaaatcgatc gcgtgatgca ggcgctgccg gatgtcaaac aagccgttac ccacgcctgt
     4801 gtgattaacc aggcggcagc cactggtggt gatgcgcgtc agttggtggg ctatctggta
     4861 tcgcaatcgg gcctgccgtt ggataccagc gcattgcagg cgcagcttcg cgaaacattg
     4921 ccgccgcata tggtgccagt cgttctgctg caactgccgc agttgccact tagcgccaac
     4981 ggcaagctgg atcgcaaagc cttaccgttg cctgaactga aggcacaagc gccagggcgt
     5041 gcgccgaaag cgggcagtga aacgattatc gccgcggcat tcgcgtcgtt gctgggttgt
     5101 gacgtgcagg atgccgatgc tgatttcttt gcgctgggcg gtcattcgct actggcaatg
     5161 aaactggcag cgcagttaag tcgacagttt gctcgtcagg tgacgccggg gcaggtgatg
     5221 gtcgcgtcaa ccgtcgccaa actggcaacg attattgatg gtgaagagga cagctcccgg
     5281 cgcatgggat tcgaaaccat tctgccgttg cgtgaaggta atggcccgac gctgttttgt
     5341 ttccatccgg cgtccggttt tgcctggcag tttagcgtgc tctcgcgtta tctcgatcca
     5401 caatggtcga ttattggcat tcagtcgccg cgccctcatg gacccatgca gacagcgaca
     5461 aacctggatg aagtctgcga agcgcatctg gcaacgctac ttgagcaaca accgcacggc
     5521 attgcggcgc ggctgcgtgc ccgtggcgaa caggtggcat ttcttggttt gctggatacc
     5581 tggccgccag agacgcaaaa ctggcaggaa aaagaggcta atggtctgga cccggaagtg
     5641 ctggcggaga ttaaccgcga acgcgaggcc ttcctggcgg cacagcaggg aagtacttca
     5701 acggagttgt ttaccaccat tgaaggcaac tacgctgatg ctgtgcgcct gctgacgact
     5761 gctcatagcg taccgtttga tggtaaagcg acgctgtttg ttgctgaacg cacgcttcag
     5821 gaaggtatga gccccgaacg cgcctggtcg ccgtggattg cggagctgga tatctatcgt
     5881 caggattgtg cgcatgtgga tattatctct ccaggggcgt ttgtaaaaat tgggccgatt
     5941 attcgcgcaa cgctaaacag gtaaattaat attatctata aatcca
//
LOCUS       DAJTSI010000189         5972 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000189 DAJTSI010000000
VERSION     DAJTSI010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5972
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.190"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..756
                     /locus_tag="OO327_003658"
     CDS             <1..756
                     /locus_tag="OO327_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6020042.1"
                     /translation="AERILPPGSFASIGQALPPGEPLSTEERIRILVWNIYKQQRAEW
                     LSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFVLPQHPSGVMTLSAAH
                     PVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNFSLGVDVYSKQLLPIG
                     DQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTDDQRRRAFGRPLDFVF
                     YRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            834..1604
                     /locus_tag="OO327_003659"
     CDS             834..1604
                     /locus_tag="OO327_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6020043.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(1652..2998)
                     /gene="mltD"
                     /locus_tag="OO327_003660"
     CDS             complement(1652..2998)
                     /gene="mltD"
                     /locus_tag="OO327_003660"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR6020044.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(3070..3825)
                     /gene="gloB"
                     /locus_tag="OO327_003661"
     CDS             complement(3070..3825)
                     /gene="gloB"
                     /locus_tag="OO327_003661"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR6020045.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            3859..4581
                     /locus_tag="OO327_003662"
     CDS             3859..4581
                     /locus_tag="OO327_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6020046.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(4578..5045)
                     /gene="rnhA"
                     /locus_tag="OO327_003663"
     CDS             complement(4578..5045)
                     /gene="rnhA"
                     /locus_tag="OO327_003663"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR6020047.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            5110..5841
                     /gene="dnaQ"
                     /locus_tag="OO327_003664"
     CDS             5110..5841
                     /gene="dnaQ"
                     /locus_tag="OO327_003664"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR6020048.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
BASE COUNT         1462 a         1468 c         1462 g         1580 t
ORIGIN      
        1 gcggaaagga tcttaccgcc ggggtctttt gcgagcatcg gccaggcatt accacctgga
       61 gaaccgttaa gtaccgaaga gcgtattcgg atcctggtgt ggaacatata caaacagcaa
      121 cgcgctgaat ggttgtcggt attaaagaac tacggcaaag atgcacatct ggtgttattg
      181 caggaagcgc agacaacgcc agagttagta cagtttgcga ccgctaacta tcttgccgcc
      241 gatcaggtgc ccgctttcgt gctgccacaa catccttccg gtgtaatgac cctttcggcg
      301 gcgcatccag tgtattgctg cccgttacgt gaacgagaac ccattttgcg tctggcgaag
      361 tcggcactgg tgacggtcta tccattacct gacacccgcc tgttgatggt ggttaatata
      421 cacgccgtca acttcagtct gggcgtggat gtctatagta agcagttact tcctattggt
      481 gatcagatag ctcaccacag cggcccggtc attatggcgg gagatttcaa tgcctggagc
      541 cgtagaagga tgaacgcgtt atatcgcttt gcgcgggaaa tgtcgctgcg ccaggtgcgt
      601 tttaccgatg atcagcgccg tcgggcgttt ggccgcccgc tcgattttgt tttctaccgt
      661 ggtctgaacg tcagcgaagc ttctgtattg gttacgcgcg cttccgatca caatccgcta
      721 ctcgttgaat tcagtcccgg caagcctgat aaataaggta tgtcaggtct gccacagggc
      781 agaccaacgt ttggcgctgt ccttttatac tcaaacaaca aaaggacagc gcaatgacaa
      841 cacaatccca ccatgaccac gtagaaaagc agtttagctc tcaagcctgc gaatatctga
      901 ccagtaccgt acatgcatcc ggacgagatt tacagcgcct ggcggtgcgt ctggctgatt
      961 atcctggtgc aagtgcactt gatatgggct gcggagcagg gcatgccagc tttgttgctg
     1021 cgcaaaacgt gagcgcggtg gtggcgtatg acttatctgc ccaaatgctg gatgtcgtgg
     1081 cacaagctgc cgaagcccgg caactgaaaa atatcaccac ccgccaggga tatgccgaaa
     1141 gtctgccatt tgccgataac gcatttgata ttgttatcag ccgttattct gcccatcact
     1201 ggcatgatgt tggtgcagca ctgcgtgaag tgaaccggat attgaaacct ggcggtagga
     1261 tgattgtgat ggacgtaatg tctccgggtc atccagtgcg cgacatctgg ttacagacgg
     1321 tagaagcatt acgcgatacc tctcacgtac gaaactacgc cagcggtgag tggttgaggt
     1381 taatcaatga agccaatctg atagttgata atttaattac agataagtta ccgctggaat
     1441 tttgttcatg ggtcgcgaga atgcgtacgc cagaagcgtt agtagacgct attcgcattt
     1501 accaacagag cgcatcgaca gaggtgagaa cgtattttgc cttgcagaat gacggctctt
     1561 tcaccagtga tatcatcatg gtagaagcac ataaagcggc ataaataaaa aaggcaccgg
     1621 gggaatcggt acctttttat tatctggttt gtcaggaatc tggcatgttg ttgtttttca
     1681 caaacaacgt cagcttgtcg cctggttgca gattcgcagt gtcgctgttc cagcgcatca
     1741 catctttgat gttcacgccg tggcgtttag caatgcttga aagcgaatcg cctttgcgca
     1801 cacgatacgt aatgctatcg ctgttgtttg ccagtcgctg tgcaccaatc gtcaaacttt
     1861 ggcctggctt cagcttagat ccgcgcagtt tgttccactg ctgcaaatct ttggtgctta
     1921 cgccgagacg tgaagcgatg cttgaaagcg tgtcgccaga gcgtacggtg taaacacggc
     1981 tgttaagcgg cgtattgttg gcaaccagcg tcgactgtac agcagcaatt tcgcctgaag
     2041 cgagagattc acgcagttga tctgcatgct tctttggcac catcacgtac tgcggcccac
     2101 ttgcgcccag cgtggagcct ttcacgccag cgttgaatgt cttcagcttg ctgacggaaa
     2161 tccccgccat atctgcaacc ttcgccattt caaccgggct actcaggtgc acacgcgcca
     2221 gagcacggct ttcatcggtc gttggcagac gtacgccata acgcttgctg tttttgagaa
     2281 tatcactcaa tgccagcatt ttaggcacgt actgcttcgt ttcctgcggc aacggtaacg
     2341 accagaagtc cgtggatttc ccacgcgctt tgttcgtttt aattgccttc atgacccgac
     2401 cttcgccgct gttataagcc gctacggtca gaagccagtc gtcgtcaaac attttgttca
     2461 gacgctgcat catattcagc gcggcagttg ttgaagcaac aacatcgcga cgcgcgtcat
     2521 aattgcgggt ctgtttcaaa ccataattgc gccccgtgct cggaatgatc tgccagatgc
     2581 ctgcggcatt ggcgccagac gttgcgtgag gatcaaaagc gctctccact atgggtagta
     2641 gtaccagttc cataggcatg ttacgttttt taacttgccc tgctatccag tacatatacg
     2701 gctctgcccg taaagttaca tcgtggagat agctcttatt gcgtaaatat ttctgtttct
     2761 gttcgcgaat ccggtcattt tccggaattc ccatctttag ctcgtcgcca atgaaagccc
     2821 acaagtcacc atctggcgcg atagacgtcc catcgtccat ccatcgtgcc tgacttgtaa
     2881 actttgctgc ttccccttga ccagctgcag aaaggctctg tgcgtgctgt tgaacgttgc
     2941 cggtactctg gcaacccacg agcaggacag aggcgagtaa tatcgctttt gccttcatgt
     3001 gtgtgtcaat agttgcttaa aagacgaccg atcataacgg cgaacggagc cgatgacaag
     3061 aaagttttat cagaacctat ctttctttga ccttaaccat gcaaaacgct cttcaggttg
     3121 ttgcaataat gtttcttcat taattccatt aattaaatca atatcttccg ttcttaaaaa
     3181 aacattaatt tgccgctcat ttttcagaat tacgggtagt gttatttgat tttttgcccg
     3241 taactcctta actttacgat aataatcatt tatggacaaa tcgtgcggaa aaatactcaa
     3301 agcaaacttc atatttgata acgtatattc atgagcacaa cataccaatg tatcgtcagg
     3361 taacgcactt agctttttaa gtgattgata catttgtaaa gctgtccctt caaacaaccg
     3421 accacaccca ccagaaaaca gcgtgtcgcc gcaaaataga taaggtttac tgaagtaaca
     3481 gatatgtcct aaagtgtgac ccggtgtggt aattacacta aattcatgcc ctaaaacgaa
     3541 ggcagtttcg ccatctttga ctacctgtgt tgttccctta tcttgtgtct cttgtggacc
     3601 atacactaca atttgcggaa acttttccac cagttctttt acgccgccaa cgtgatcgtg
     3661 gtgatggtgg gttagaaata tggcctccgg ttgccagtta ttggcggtaa tggcgtttaa
     3721 tactggctct gcgtctccgg gatcgacaat caggcagcga cctgcctcat cattcaaaac
     3781 ccagatgtaa ttgtcatcaa aggcgggaat actgttaaga ttcatagatt acctctcagt
     3841 gtaaaacgga aggttgtgat gaaaccggca agagtccctc aaactgtcgt ggctcctgat
     3901 tgctggggcg atttgccctg gggagagctt tatcgcaagg cgctggagcg ccagctcaac
     3961 ccgtggttca ctaaaatgta tggttttcat ctgcttaaga ttggcaattt aagcgcagaa
     4021 atcaattgcg aagcgtgcgc ggtttctcat caagtgaatg tttctgcgca aggaatgccc
     4081 gtccaggtgc aggcggatcc acttcatctt ccttttgccg ataaatccgt tgatatttgt
     4141 ctactggcac ataccttgcc gtggtgcacc gatccgcatc gtttattacg tgaagccgat
     4201 cgggtactga ttgatgatgg ctggctggtg attagtggct tcaaccccat cagtttaatg
     4261 ggattacgca aacttgtgcc ggtattgcgc aaaacctcgc cctataatag ccggatgttt
     4321 actctgatgc ggcaactgga ctggctctct ttgttgaatt ttgaagtgct gcacgccagc
     4381 cgtttccacg ttctcccgtg gaacaaacac ggaggaaaac tattgaatgc gcatattcct
     4441 gcgcttggtt gcttacaact tattgttgcc cggaaacgga ctattccttt aacgctaaat
     4501 ccgatgaaac agagtaaaaa caagccacga attcgccagg cggttggagc cacccggcaa
     4561 tgtcgtaaac cacaggctta aacttcgact tggtagcccg tatcttccag tgtgggattc
     4621 atcgccgcgg cacgagccag ttcatcacag cgttcgtttt ccgggtgtcc ggcatggcct
     4681 ttaacccatt cccatttgat ttgatgctgc cccaatgcag catcaagacg ttgccagaga
     4741 tcgacatttt ttactggttt tttgtctgct gttttccagc cacgtttttt ccagttatgg
     4801 atccactggg tgataccctg gcggacatac tggctgtcgg tactcaaaat gacttcgcaa
     4861 tgttctttta acgcctccag cgcgacaata gcggccatca actccatacg gttgttggtg
     4921 gtgcgggtgt agcctgcgct aaaggttttc tcgcgtccgc gatagcgtaa aatagcgccg
     4981 taacccccag gtccaggatt acccagacac gaaccatcgg tgaaaatttc tacctgttta
     5041 agcatctctg gtagacttcc tgtaattgaa tcgaactgta aaacgacaag tctgacataa
     5101 atgaccgcta tgagcactgc aattacacgc cagatcgttc tcgataccga aaccaccggt
     5161 atgaaccaga ttggtgcaca ctatgaaggc cacaagatca ttgagattgg tgctgttgaa
     5221 gtagtgaacc gtcgcctgac gggcaataac ttccatgttt atctcaaacc cgatcggctg
     5281 gtggatccgg aagcctttgg cgtacatggt attgctgatg aatttttgct cgataagccc
     5341 acgtttgccg aagtagccga tgagtttatg gactatatcc gcggcgcgga gttggtgatc
     5401 cataacgcag cgttcgatat aggctttatg gactacgagt tttcgttgct taagcgcgat
     5461 attccgaaga ccaatacctt ctgtaaggtc accgatagcc ttgcggtggc gaggaaaatg
     5521 tttcccggta aacgcaacag cctcgatgcg ctatgtgccc gctacgaaat agataacagt
     5581 aaacgaacgc tgcacggggc attactcgat gcccagatcc ttgcggaagt ttatctggcg
     5641 atgaccggtg gtcaaacgtc gatggctttt gcgatggaag gagagacaca gcagcaacaa
     5701 ggtgaagcaa caattcagcg cattgtacgt caggcaagta agttacgcgt tgtttttgcg
     5761 acagatgaag agctggcagc acatgaagcc cgtctcgatc tggtgcagaa aaaaggcgga
     5821 agctgcctct ggcgggcata aatacatgta aaaggcgcta aaaatagcga cttgggcgat
     5881 ttttgcagca aacgattcaa aagatgagaa aaatcgttga cgaaggtcga ggcaatccgt
     5941 aatattcgcc tcgttcccaa cggaacacaa cg
//
LOCUS       DAJTSI010000190         5917 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000190 DAJTSI010000000
VERSION     DAJTSI010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5917
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.191"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1025)
                     /locus_tag="OO327_003665"
     CDS             complement(<1..1025)
                     /locus_tag="OO327_003665"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="catalyzes the formation of
                     2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine from
                     N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide and
                     L-glutamine in purine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR6020049.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLR"
     gene            1284..2840
                     /gene="mltF"
                     /locus_tag="OO327_003666"
     CDS             1284..2840
                     /gene="mltF"
                     /locus_tag="OO327_003666"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR6020050.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(2837..3373)
                     /gene="tadA"
                     /locus_tag="OO327_003667"
     CDS             complement(2837..3373)
                     /gene="tadA"
                     /locus_tag="OO327_003667"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR6020051.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(3398..4033)
                     /gene="yfhb"
                     /locus_tag="OO327_003668"
     CDS             complement(3398..4033)
                     /gene="yfhb"
                     /locus_tag="OO327_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR6020052.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            4242..5090
                     /locus_tag="OO327_003669"
     CDS             4242..5090
                     /locus_tag="OO327_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR6020053.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(5429..5917)
                     /locus_tag="OO327_003670"
                     /pseudo
     CDS             complement(5429..5917)
                     /locus_tag="OO327_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT         1497 a         1530 c         1473 g         1417 t
ORIGIN      
        1 attcgcaggt tggatacgga gaaaccaacc aaaccagctt tcggcttcgc gccgcgcccg
       61 gtggcacctt catcgcggat ttcaccgccg gaaccggtcg ccgcgcccgg ccacggagaa
      121 atcgccgtcg ggtggttgtg agtttcgact ttcatcagga tgtgcgccgg ttcctgatgg
      181 aaattgtagc ggcccgtttc gtggtcagca aagtagcggc ccacttcaga accttccatt
      241 acggcggcgt tatctttata agcagagaga acgtgatctg gcgtggtttc gaaagtattt
      301 ttgatcatcc tgaacagcga tttcggctgt tgttcaccgt cgataatcca gtcggcgtta
      361 aaaattttgt ggcggcagtg ctcagagttc gcctgagcga acatatacag ctcgatgtcg
      421 ttcgggttac gaccaagctt tgtgaaagca tcctgcagat agtcaatttc atcttccgcc
      481 agagccaagc caagacgcag gttagcgtcg atcagcgcct gacggccctg ccccagcaaa
      541 tcaacgcttg tgaccggagt cggttgatgg tgggcaaaca actgctctgc atcatctaaa
      601 tcaaaaaaga ccgtttccat catgcggtcg tgcagttcag cggtaacctg ctgccattgt
      661 tcattggtca gcgtaccggc ttctatatag taagcaacgc cgcgctcaag gcggtttacc
      721 tgttgtagcc cgcagttatg ggcaatatcg gtcgctttcg aagaccaggg agagatggtg
      781 ccaggacgcg gggtcaccag caggagtttg ccttgcgggg cgtggctggc gagtgccgga
      841 ccatatttca gcaggcgttc aagttgtgcg tgctcatcat cgtttaacgg cgcattgagg
      901 tcagcaaaat ggacatactc ggcgtaaata ttgtgaaccg ggagcctggc agcctgaaaa
      961 cgtgccagca gtttgttgat tcggaatgcc gacagtgcag gcgaaccacg cagaatttcc
     1021 atcataagtc tctcgtcttc taagctttcg gtgtacccaa ggggggggaa acgggcgtca
     1081 ttataaagaa tctgatgcgc tgacgaaacc gtttgcgtgg aaataaaatc accatcgtga
     1141 attagcaacg cgtgccgcca atggctgtaa taagttgcca tctggcgcag gtttacgcaa
     1201 aatgccgctc atttatgagt aaacctttca ctattattac gttttttcaa gctgggacgc
     1261 gcacgacaca gagaattaac taattgaaaa aattaaagat taattatctg ttcatcggca
     1321 ttctggcact gctgctcgcg gtcgctctct ggccatccat tccctggttt ggtaaagccg
     1381 acaaccgtat cgccgccatt caagcgcggg gagagttgcg tgtgagcacc attcatactc
     1441 ccctgactta taacgaaatc aacgggaaac cttttggcct ggattacgaa ctggcgaaac
     1501 agtttgccga ttacctcggc gtaaaactga aagtgaccgt gcggcagaat atcagccagc
     1561 tgtttgacga ccttgataat ggtaacgccg acctgctggc ggcaggactg gtctataaca
     1621 gtgagcgggt aaaaaattat cagcctggcc ctacctatta ttccgtgtca caacaactgg
     1681 tttataaagt gggtcagtat cgcccacgta cgctgggcaa cctgacggcg gagcaactca
     1741 ccgttgcacc gggtcatgtg gtagttaacg atctccagac cctgaaagaa acaaaattcc
     1801 cggaattaag ctggaaggta gacgacaaaa aaggctctgc ggaattaatg gaagatgtca
     1861 tcgaaggaaa actcgattac accattgctg attctgtcgc catcagcctg tttcagcgcg
     1921 ttcacccgga gctcgccgta gcgctcgata tcaccgatga acaaccggtg acttggttta
     1981 gcccgttaga tggcgataat accctttccg ccgccctgct cgacttcttc aacgaaatga
     2041 atgaagacgg tacgctggca cgcattgaag agaaatacct ggggcatggc gatgattttg
     2101 attacgtcga tacgcgcaca tttttacgcg ccgtcgatgc ggtactgccg cagttaaagc
     2161 ccctgtttga gaaatacgcc gaagaaattg actggcgttt gctggccgct attgcttatc
     2221 aggaatcgca ctgggatgca caggccactt caccgacggg tgtgcgcggc atgatgatgt
     2281 taaccaaaaa taccgcgcaa agcctcggca ttacggatcg taccgatgcc gaacagagca
     2341 tcagcggtgg cgtgcgttat ttgcaggata tgatgagtaa agtgccggaa agtgtgccgg
     2401 agaacgaacg gatctggttt gccctcgctg cgtacaatat gggctatgcg catatgctgg
     2461 atgcccgcgc cctgacggca aaaaccaaag ggaatcctga cagttgggct gacgtaaaac
     2521 agcgtctgcc tttacttagc cagaaaccct attacagcaa gctgacttac ggctacgctc
     2581 gtgggcatga agcctacgct tatgtcgaaa atattcgtaa gtatcagatt agcctggtgg
     2641 gttatctgca agagaaagag aagcaggcta cagaagcggc gatgcaactg gcgcaggatt
     2701 atccggcggt atcgcctacg gagttgggca aagagaaatt tccttttctc tcgtttcttt
     2761 cccagtcgtc atcaaactat ttgacccatt ctccctctct gctgttttcc aggaaaggga
     2821 gtgaagagaa acaaaattaa tccgtcgagg attgcgcttt tttctgcgct ttaatttcct
     2881 ggcggcgcat gcgaaagaag tcactgagca acgccgcgca ctcatccgcc agtattcctt
     2941 ccgtaatttc cactcggtga ttcatacccg gatgatgcag cacatccatt aaagatcccg
     3001 cagcgccagt tttcgcgtca cgcgcaccaa agaccacgcg accaatgcga ctgtggatca
     3061 tcgctccggc acacattaca catggttcaa gcgtgacata caacgtggcg tcgatcagac
     3121 gataattttg catcaccaga ccaccctgcc gcagggccat gatttctgca tgtgcggtgg
     3181 gatcatggcg accaatcggg cggttccagc cttcgccgat tacccgattg ttatgcacta
     3241 ataccgcgcc gaccggcact tcccgctcat cccaggcacg tttcgccagc gtcagcgcgt
     3301 gacgcatcca atattcgtgg ctaaattcga cttcagacaa aaagaaaact ccggttataa
     3361 aagcgcggcg cattatacac ggacgctatg ctttactcta ttccagttgc tggagttcac
     3421 cgcgcggggt tactcgccaa cgatgctgac agaaataaag cagcggattg tcctgattac
     3481 tgtcgctata gccactgtac agccgcagcg gcgtgccgat tttgcgctcc agttgtgcga
     3541 ccttttcatg tcccagacaa cgcatcgtca atacccaacc accatagcca cgctgaattt
     3601 ggctggcgat aagattaacc cgcggcagcc agggcgtatc gaaataaacc gcttcaacca
     3661 gcggctgcgg agagccggta atcaaccaga tatcagcatc ggaacttaac aggtaggtgg
     3721 ttaatcgctc ctgaaccagc ggaaaggcgg taacattgtc gcgaaaccag cgcacgaaat
     3781 cggcctgcaa cgtctgtaaa cgtgcttcgc tgtgaccaaa agtgcacccc cacagaagca
     3841 gactcatcgg ccagcgtgcc gcacgacctt ttatcaataa cgcaatggct ataatcggta
     3901 acaacggcag gacaagtaac gcattcagcg gttggcgacg cagtaaatag cgcagaaaac
     3961 tgccgaacat atcctgctga tgcaatgttc catctaagtc aaaaaacacc acacgacgct
     4021 cgtgagttgc caaaccatac tcctctggac tgaaacatcc tgattaattt tcgctactac
     4081 atagcctaac agatagatca tcacttttcc ggcaacaatc gaccgcatca tggctaactg
     4141 gaatttttaa ttcatggcaa ttagcggcaa tggaatataa aattcactcg cgtgtgtctc
     4201 atattgcctg ttgtcgccac tatttcatcg cgaggaaaga tatgaacggc ttacttcgta
     4261 tccgtcagcg ttaccaaggg cttgcccaga gtgataaaaa actggcggat tatctgctgc
     4321 tacaacctga tacggcgcgc catttaagct ctcagcaact ggccaacgaa gccggagtca
     4381 gtcagtccag cgtcgtgaag ttcgcgcaaa aactcggcta taaaggtttt ccggcgctta
     4441 agttggcgtt gagtgaagcg ctggcaagcc agccggaatc gccctccgtg cccattcata
     4501 accaaatccg cggtgatgat ccgttacggc tggtcggcga aaaactgatt aaagaaaata
     4561 ccgccgctat gtacgcaacg ctaaacgtta atagtgaaga gaaactgcat gaatgcgtaa
     4621 caatgttgcg ctctgcgcgg cggataattc tgaccggtat tggcgcttcg ggtctggtgg
     4681 cgcaaaactt tgcctggaag ctgatgaaga ttggcttcaa tgctgccgca ctgcgcgata
     4741 tgcatgcgct actcgcaaca gtacaggcgt cgtcccctga cgatctgtta ttagccattt
     4801 cctacaccgg tgtacgacgc gagttaaacc tggcggcaga tgagatgctg cgagtgggcg
     4861 gaaaagtgct ggcgattacc ggctttactc cgaatgccct gcaacagcgt gcttctcatt
     4921 gcctgtatac cattgccgaa gaacaagcga caaacagtgc ttcaatctct gcttgtcacg
     4981 ctcagggaat gttaacggat ttgctgttca ttgcgctgat tcagcaggat ctggaactgg
     5041 caccagaacg tattcgtcat agtgaagcgc tggtgaaaaa actggtctga gtaaagaatg
     5101 cgcgtataat gcccgcccgg tttgtgttgt tttgagagtt tccttatggc gctgttaatc
     5161 actaaaaaat gcatcaattg tgatatgtgt gaaatcaaat aaaaacacat ttattttcat
     5221 agtgatacag caatgtgtta ttgaatgtgt tgttggattt atggtgcaaa aaaattttgc
     5281 ataagttcag caacacaaag aacaccactt tctttaacgt cctccgcgcc ctaaaggacg
     5341 gggctttacg gcgcaaaagg cctgtgacat acaacattaa cttttcagtc aaccgtagca
     5401 cgttcttgta tacgccgtgt ctgcggtttt atataaccct ggctggaaac ctcttataca
     5461 aagtcgattc gccaacatca tagatgatcg ccaccttctg gcgagaaact cctgatgcaa
     5521 ttagtcgccc ggcctgcgcc cattattctg gtgaaagttt gggacggcga ccgccaattc
     5581 gtccctgtgc gcgagcagct tccagtcctg cttttgttcg ttcaacaatc agttcacgtt
     5641 ccatttcagc cagggcaccc atcacatgga agaaaaagcg tcccattggt gtgctggtat
     5701 caattgaatc agtcagacta cgaaagttga tgcctcgttc gcgcaacgcc tccaccagca
     5761 cgacaagatg ccgcatactg cgccccagcc gatccagctt ccagacaacc agtgtgtcgc
     5821 ctgccgataa tgtcttgagc agttttttca gccccggtct ttcggacttt gtaccactta
     5881 tcttgtcttc aaaaatcagc tcgcatcctg cacagtt
//
LOCUS       DAJTSI010000191         5889 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000191 DAJTSI010000000
VERSION     DAJTSI010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5889
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.192"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            50..244
                     /locus_tag="OO327_003671"
     CDS             50..244
                     /locus_tag="OO327_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HCR6020054.1"
                     /translation="MLARKGVETVLRLLDKETQELLQLEGRKRLAALMLVDMPDEQVR
                     EILEEIEASILSESVNAKAG"
     gene            511..1437
                     /gene="tauA"
                     /locus_tag="OO327_003672"
     CDS             511..1437
                     /gene="tauA"
                     /locus_tag="OO327_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6020055.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            1450..2217
                     /gene="tauB"
                     /locus_tag="OO327_003673"
     CDS             1450..2217
                     /gene="tauB"
                     /locus_tag="OO327_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR6020056.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            2214..3041
                     /gene="tauC"
                     /locus_tag="OO327_003674"
     CDS             2214..3041
                     /gene="tauC"
                     /locus_tag="OO327_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR6020057.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            3038..3889
                     /gene="tauD"
                     /locus_tag="OO327_003675"
     CDS             3038..3889
                     /gene="tauD"
                     /locus_tag="OO327_003675"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCR6020058.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRTVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="OO327_003676"
     CDS             complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="OO327_003676"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR6020059.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            5493..5861
                     /locus_tag="OO327_003677"
                     /pseudo
     CDS             5493..5861
                     /locus_tag="OO327_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         1401 a         1454 c         1597 g         1437 t
ORIGIN      
        1 agaaatgttg tcgtttctta ccagtgagga acgagaagcc cttacaaaca tgctcgcaag
       61 gaaaggtgtt gagactgtgc tacgcttgtt agataaggaa actcaagagc tactgcaact
      121 agagggccgt aaacgcctgg cagcgttgat gctcgttgat atgcccgacg agcaagtcag
      181 agagattttg gaagagattg aagctagtat tctctcagag agtgtaaacg cgaaggcggg
      241 ttaacttcat tgacctgcct ttagggtgcc tttaaaattc gtttttaaag gctacgatga
      301 catttttgaa gagtcttaaa agaattaaaa tacacggcgc gcctatcttg aatgttttat
      361 cgatatcgaa gcattgagca tttttaactc gttttatctt gaatgcaatc ggcgcataac
      421 ttattgaatc ccatcaaatc ccgtcttttt ttacccattc ccggttatct tgaataaatt
      481 acctagtcat ataacacact tcttgccgca ctggcattca tcgcttttca ggcacaggcg
      541 gtgaacgtca ccgtggcgta tcaaacctca gccgaaccgg cgaaagtggc tcaggccgac
      601 aacacctttg ctaaagagag cggggcgagc gtggactggc gtaagtttga cagcggagcc
      661 agcatcgtgc gggcgctggc ttctggcgac gtgcaaatcg gcaacctcgg ttccagccca
      721 ttagcggttg cagccagcca acaggtgccg attgaagtct tcttgctggc gtcaaaactg
      781 ggtaactccg aagcgctggt ggtaaagaaa actatcagca aacccgaaga tttgattggt
      841 aaacgcattg ccgtaccgtt tatctccacc acccactaca gcctgctggc ggcgctgaaa
      901 cactggggga ttaaaccagg gctagtggag attgtgaacc tgcagccgcc cgcgattatc
      961 gccgcatggc agcggggtga tattgatggt gcttatgtct gggcaccggc ggttaacgcc
     1021 ctggaaaaag acggcaaggt gctgaccgat tctgaacagg tcgggcagtg gggtgcgcca
     1081 acgctggacg tctgggtggt gcgcaaagat tttgccgaga aacatcctga ggtcgtgaaa
     1141 gcgttcgcca aaagcgccat cgatgcacag caaccgtaca ttgctaaccc tgacgcgtgg
     1201 ctgaaacagc cggaaaacat cagcaaactg gcgcgtttaa gcggcgtgcc tgaaggtgac
     1261 gttccggggc tggtgaaggg aaatacctat ctgacgccgc agcaacaaac ggcagaactg
     1321 accggaccgg tgaacaaagc aatcatcgac accgcgcagt ttttgaaaga gcagggcaag
     1381 gtcccggctg tagcgaatga ttacagccag tacgttacct cgcgcttcgt gcaataaaag
     1441 gaggcgcaga tgctgcaaat ctctcatctc tatgccgatt atggcggcaa accggcactg
     1501 gaagatatca acctgacgct ggaaagcggc gagctactgg tggtgctggg gccgtccggc
     1561 tgcggtaaaa ccaccctgct gaatctgatt gccggttttg tgccttatca gcatggcagc
     1621 attcaactgg cgggtaagcg tattgaggga ccgggagcag agcgtggcgt agtttttcag
     1681 aatgaagggc tgctaccgtg gcgcaatgta caggacaacg tggcgttcgg cctgcaattg
     1741 gcaggtatag agaaaatgca gcgactggaa atcgcgcacc agatgctgaa aaaagtgggg
     1801 ctggaaggcg cagaaaaacg ctatatctgg cagctttccg atggtcaacg tcagcgggtg
     1861 gggattgccc gcgcgctggc ggcgaatccc cagctgttat tactcgacga accgtttggt
     1921 gcgctggacg ccttcacccg cgaccagatg caaactctgc tgctgaaact ctggcaggag
     1981 acgggcaagc aggtgctgtt gattacccac gatatagaag aagcggtgtt tatggcgact
     2041 gaactggttc tgctttcatc cggccctggc cgtgtgctgg agcggctgcg gctcaacttt
     2101 gctcgccgct ttgttgcggg agagtcgagc cgcagcatca agtccgatcc acaattcatc
     2161 gccatgcgcg aatatgtttt aagccgcgta tttgagcaac gggaggcgtt ctcatgagtg
     2221 tgctcattaa tgaaaaactg cattcgcggc ggctgaaatg gcgctggccg ctctcgcgtc
     2281 aggtgacctt aagcattggc acgttagcgg ttttactcac tgtatggtgg acggtggcgg
     2341 cgctgcaact gattagcccg ctgtttttgc cgccgccgca acaagtactg gcaaaactgc
     2401 tcaccattgc cggaccgcag ggatttatgg acgccacgct gtggcagcat ctggcagcca
     2461 gcctgacgcg cattgtgctg gcgctactcg cggcggtggt gatcggtatt ccggtcggga
     2521 tcgccatggg gctaagcccg acggtgcgcg gcattctgga tccggtaatc gaactttatc
     2581 gtccggtgcc accgctggct tatttgccgc tgatggtgat ctggtttggt attggtgaaa
     2641 cctcgaagat cttactgatc tatttagcga tttttgcgcc ggtggcgatg tcggcgctgg
     2701 caggggtgaa aagcgcgcag caggttcgca ttcgtgccgc ccagtcgctg ggtgccagcc
     2761 gtgcgcaggt gctgtggttt gtcattttgc ccggcgcgct gccggaaatc ctcaccggat
     2821 tacgcattgg tctgggtgtg ggctggtcta cgctggtagc ggcggagctg attgccgcga
     2881 cacgcggttt aggatttatg gttcagtcag cgggtgaatt tctcgcgact gacgtggtac
     2941 tggcggggat cgcggtgatt gcgattatcg cctttctttt agaactgggt ctgcgcgcgt
     3001 tacagcgccg tctgacgccc tggcatggag aagtacaatg agtgaacgtc tgagcattac
     3061 cccgctgggg ccgtatatcg gcgcacaaat ttcgggtgcc gacctgacgc gcccgttaag
     3121 cgataatcag tttgaacagc tttaccatgc ggtgctgcgc catcaggtgg tatttctgcg
     3181 cgatcaagct attacgccgc agcagcaacg cgcgctggcc cagcgttttg gcgaactgca
     3241 tattcaccct gtttaccctc atgccgaagg ggttgacgag atcatcgtgc tggataccca
     3301 taacgataat ccgccggata acgacaactg gcataccgat gtgacattta ttgaaacgcc
     3361 acccgcaggg gcgattctgg cagctaaaga gttaccttcg accggcggtg atacgctctg
     3421 gaccagcggt attgcggcct atgaggcgct ctctgttccc ttccgccagc tgctgagctg
     3481 gctgcgtgcg gagcatgatt tccgtaaatc attcccggaa tacaaatacc gcaaaaccga
     3541 ggaggaacat caacgctggc gcgaggcggt cgcgaaaaat cctccgttgc tacatccggt
     3601 ggtgcgaacg catccggtga gcggtaaaca ggcgctgttt gtgaatgaag gctttactac
     3661 gcgaactgtc gatgtcagcg agaaagagag cgaagccttg ttaggctttt tattcgccca
     3721 tatcactaaa ccggagtttc aggtgcgttg gcgctggcaa ccgaatgata ttgcgatttg
     3781 ggataaccgc gtgactcagc actatgccaa tgccgattac ctgccacagc ggcggattat
     3841 gcatcgggcg acgatccttg gggataaacc gttttatcgg gcggggtgat acgagtgcgg
     3901 acggttccct cgcccccttg gggagagggt tagggtgagg gggcgttcac tgtactttca
     3961 acaggttaac tccccctttc tgagaggaaa aaaattaacg cagaatcttc ttctcagcca
     4021 aatccagcgc aaagtagctg aaaatcagat ccgcacccgc acgcttaatt gaacctaagc
     4081 tttcgagcac gactttctct tcatctatag cacccgccag cgcggcgaac ttaatcatcg
     4141 cgtactcacc gctcacctga tacgcgccaa tcggcaattc agtacgttca cgcagctcac
     4201 gcacgatgtc gaggtacgct ccagcaggtt taaccatcag gcagtctgcg ccctgggctt
     4261 catccagcag tgactcacga atcgcctcgc gacggttcat tgggttcatc tgatagcttt
     4321 tgcggtcgcc ttttaatgcg cttcctgcag cttcacggaa cgggccataa aaggaagagg
     4381 cgaacttggt cgaatacgac ataatcgccg tatctttaaa gcctgcagcg tccagcgcct
     4441 gacgaatcgc ctgtacctgg ccgtccatcg cggcggaagg ggcgatgaag tctgcgcctg
     4501 cagcagctgc aaccacggct tgcttgccta aattttccag agtcgcgtcg ttgtcgacgc
     4561 catgctcgca cagcacaccg cagtgaccgt gagaagtgta ttcacagaaa caagtgtctg
     4621 acatgacgat catttctggc acagtctgct tgcagatgcg cgacatacgc gccaccagtc
     4681 catcttcccg ccaggcgtcg ctgccggttt catcggtatg gtgagagatg ccaaaagtca
     4741 ttacggaacg gataccggcg ttggcgatgc gttcaatttc gcgtgccaga tgtttctctg
     4801 gaatgcgcat cacgcctggc atggcttcaa cggctttgta gtcgtcaatt tcttcttcaa
     4861 caaagatcgg caacaccagg tcgttaaggc taagtgttgt ctcttcaaac atagcgcgca
     4921 gcgcaggaga tttgcgcagg cgacgagggc gttggattaa gtctgtcatg gtctgcctga
     4981 tgtttgtgga attaaggtgc acagtatacc tgaagcgggg caggagtgtt ttacgaaagt
     5041 tgtcgcgatg ttgacaagaa gagaatgaaa gagagaccag gacatctttg taatagaaat
     5101 agcaataatt tcatattcac tgaaaatatt tttaatcttt atttatagct cttgattatt
     5161 attttttggc gtttggttgt gctgctataa attgaataat tcaaactttg ttgtatattg
     5221 ataagatatt tatctggttt gtatttttga tatttataac gcgtatgcat caaaaataac
     5281 attatctgca acatactgaa atatcgtcct tttatcaatt tgatgcgtgt ttttctccat
     5341 aaattatata tgtccacatt cggacttagg ggaaagaata attgaaccat tcgtctgtaa
     5401 cgcagcataa tcgttagcgc gaaacataat atgttttcta tgcattgata attgatggat
     5461 caacttatta cgtccctgag gagggatgac aaatgcactc ctggaaaaag aaacttgtag
     5521 tatcacaatt agcattggct tgcactctgg ctatcacctc tcaggctaat gcagcgacca
     5581 acgatatttc tggtcaaact tacaatactt tccatcacta caacgacgcc acctatgctg
     5641 atgacgttta ctatgatggt tatgtaggct ggaacaacta tgccgctgat agctattaca
     5701 acggcgatat ctacccggtc attaataacg ctaccgttaa cggagtgatt tctacctact
     5761 atctggacga cggtatttct accaatacca acgccaatag tctgacaatc aaaaacagca
     5821 ctattcacgg tatgatttat tccgagtgca tgacaactga ttagactggc cccctgaatc
     5881 tccagacaa
//
LOCUS       DAJTSI010000192         5870 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000192 DAJTSI010000000
VERSION     DAJTSI010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5870
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.193"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(130..1176)
                     /locus_tag="OO327_003678"
     CDS             complement(130..1176)
                     /locus_tag="OO327_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HCR6020060.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(1190..1749)
                     /gene="tssG"
                     /locus_tag="OO327_003679"
                     /pseudo
     CDS             complement(1190..1749)
                     /gene="tssG"
                     /locus_tag="OO327_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(1746..3629)
                     /gene="tssF"
                     /locus_tag="OO327_003680"
     CDS             complement(1746..3629)
                     /gene="tssF"
                     /locus_tag="OO327_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCR6020061.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPMAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(3645..4139)
                     /gene="tssE"
                     /locus_tag="OO327_003681"
     CDS             complement(3645..4139)
                     /gene="tssE"
                     /locus_tag="OO327_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCR6020062.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(4136..4966)
                     /locus_tag="OO327_003682"
                     /pseudo
     CDS             complement(4136..4966)
                     /locus_tag="OO327_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(4953..5867)
                     /gene="tagK"
                     /locus_tag="OO327_003683"
                     /pseudo
     CDS             complement(4953..5867)
                     /gene="tagK"
                     /locus_tag="OO327_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         1226 a         1615 c         1732 g         1297 t
ORIGIN      
        1 tattcagaaa gcccgaatca gtaatgattc gggctttcgt cgttttggtg aagtgagaat
       61 ttttcactcc gaagtaaaac ggatatgatt taaaaaatgg ccagacatta taaattctgg
      121 ccacttctgt tactccagcg catcccattt ctccagcagt tctactggaa tttcatttat
      181 tacctccgag acgcgttttc cgaccaggcg ttctgcctgg cgcagcaaag gaatggtcgg
      241 gctgctcggt tcgctgtgct caaaccagcg acggatgaca cgcaggcgtt ccagcgcgtc
      301 gtttcggtcg cggatctgcc ggggttctgc gccggtcatc ggtgatattt cctccgcgat
      361 atgcaccggt agcgcaatct gctctgcctg catctgttca gtatgaacga tggtggcgtt
      421 ttccggctgc tcagcctgtg gttgtggcag gatctccggc gtggtgctct gcactgctcc
      481 cggtaacagg gccagcagtt gcgtcagacg ggagaagtcc ggtgcctggt cattcagcgt
      541 ttcacgcgca tggcgttgca gccgctcagt ggtctctgct gcctggcgaa aggcatccag
      601 cggcagtgtg ccgcgcgctt ccaggtctgc cagttgctgg cgtaccgatt ctggtgccag
      661 cgcatccgcc gggcgtaatg cagatagcga acgctccaca tcccgcacct gaaggcgcat
      721 ggcagcatta tttgacagtg taatgccacg aatatccgcc attacacctt cgtggtccag
      781 caacgccgcc agcgcgttac tgcgtgccac cgcagcatct tcggcggtga tatcttcagt
      841 cgcgagcagt tgaggatgga tggcgtccgg gtacgtggag cacaacgttt ccagcagcgt
      901 gagcgcctcc gacagtcccc tggcaccggc ctgctggata cggcagcgca gcaggacaac
      961 cagaatgcgg atatctttgc tgcgcatcag cagacgatgg gcgtcccgct caagctccgc
     1021 ccagttaatg ttttccggcg tactgacaaa atcgccatat tgcgcttctt cacgcggtgc
     1081 ggcgcgggtg aacagcagca ggtattccgg atcgtactcc agatccgggc cgcaggggca
     1141 ttccgccgaa attgggtcag acaggaatat ttccatttaa ttatcctcag tgcgccagcg
     1201 tggtggccag accgtccgga ttgctggact cacgcaccag atgggccgat gccgccagac
     1261 gagcgtgatc tggcaacggg ctgtcagcgc tctcccgcaa atcctgcccg ctgaggctgg
     1321 cgacataaaa ggcaaagcgg tcatgttgcg gtttatccag accgccccca gcggactggg
     1381 cgctgcgcca tgcctggtaa aactgcgtca gccagcggtg atgaaaaaga tcagaaaaac
     1441 gggtcagcgt cggctttcag tccggttacg cgccagttcg gtgtagtgca gcggcagtgg
     1501 accgttcggc ccccacagca cgaggctgta cagactgatg tgcaggcgcc cttcctgcca
     1561 gcccacactg gcgatttctc gtggcgcaaa ggtcagcgcg ggcgtttgtc cgaggcgaaa
     1621 tttttccatc cgtggttgcc aggtgttgcc cgccggaacg gtacacaact gcacatccac
     1681 gcggcgcatc aggttcagaa aaccgtaacg ccaggggggg gcctgcgcct gagtgaaatc
     1741 gttgctcata cgcttccccg ctgtcccgcc ctgacaggcc aggtcataat tttgccgcgc
     1801 tgcatgctgt gcagcgtcat ctgcgagaag gtattaatgg aaacatggcg ggcgatatag
     1861 tgttccagca ccaggccgaa cagatacgga ctgatgccgg aaaaaccttc ttcatccacc
     1921 gtcagttcac aactgacgcc acgcccatag accagtagtc cggaaccggg cagacggcgg
     1981 gtgaccgggg tcgttttaca accaatcagg ctgcgcacct ggcgcgattg cggactgtca
     2041 tgagcaggaa taaacaggtt cagcagatct cgcaacgcct ggccgccggt acggtgatcc
     2101 agatcggcca gcggcagata attaaaagat aactggcgga tcaaccgcca tgccatctca
     2161 cgttcagcca tcgggggctg cggcgagcgt ggcgggcgga taagccctac gcccgccacc
     2221 gggatggctg catccacggt tagatcgtcc cggccattgc gggcaataag acagggcaga
     2281 tcgcggttag tgaccatggc cgtgatggtg atgtggcgca gattttccgg gtacggcgct
     2341 tcatactggt caaccagcga caggaacact tccgagcccg tatagggggt acgggtgccg
     2401 taacggcggg cgttttctga taagcggcgg ggctcccggc gcagggaaaa ataacgtccg
     2461 tgattgcctt catcattatt gcgggtgtgg tagagcgggc gaaaggccat cttccgtgtg
     2521 gtgtcggttt ccagcccttc cacttcctgg accgagaaca cctcataatc cagcggatgg
     2581 gttctgtcca ccaccagatg ctgttccgtc gtgctgtgcg tgacatcgat ccgggcggtg
     2641 acgcggggaa acaggttgat aaccggggta cagaacaggc tgaactgtgc cgcatttgtc
     2701 tggtggatca gccagtccgg cggcaggcgg ttcagcagga taacaatctc cgccacgcca
     2761 ccgtcaattt tctgtaatcc ggcagacagg cctgtcgggg tgaaaaaata gaagcgttcc
     2821 ggacaggcaa aatattcatg cagcaggtta tgaccgtgaa acacgttcca cgccagcggc
     2881 agcagcccct gatccggctc caggccttcg tacataacgg gctgttgcag attcacatcc
     2941 agcgcgccgt caaaatgccc cggaatgcca gccaggggcg cgatggcgct ggtatgtaga
     3001 agttctaaca ggtgtgaggc cgtacgctcc tcgccgcaga gataaaacgg cagcctgtcc
     3061 agtccggcaa gctgactgaa cgttagttca ccaaaggtgc gcagggtaat acgcagcgcc
     3121 ccggcgacgt gaatgttggc cggcaggtag cggtgcaggg caggcatatc cggtggcgcg
     3181 gcggtcagtc gcgcctcttc gatggcgagc ggccacagcg ttacatcctg gctgctgcga
     3241 aactggcagg cagtgctttc gccttccgga atggcggaga aaaaggctgt gtcgcgcggg
     3301 acggtaaatc ctttcgccag atcgccttct tccgtatcgg gatgcaactg cgccacgctc
     3361 attgagggcg tgggtgtgac gtaattgggg ctgaccactt ccagcaggcg ctgggtgaaa
     3421 cgggggaatt cagcgtcaat ttttagctgc gtgcgggcgg tcaggaagct gaaggcctcg
     3481 accattcgct ctacatacgg atcggcaata tccgttccct gcatgcccag ccgggcggcg
     3541 actttgggat gacgggcggc aaattcggct cccgtttccc gcagatagct cagttcgcgg
     3601 ttgtagtatt ccagtagccg tggatccatc ggtctctctc tgaattaaaa aaacgtaatg
     3661 cgactctgct ccatatccag cgcgctacgt acccggaatg cagtcggata cggctgcgtg
     3721 aggatctcgc cgcgaatttc aaactgtaat gtgttatggc cactctggcg gcgctcatcc
     3781 tgaagtggga tgatttgcag agtggacgct ttcagccggg gttcaaaccg ggtgatggtt
     3841 cgcaaaatgg ccctgcggat atcgtcccac ttgtgctcat acagaaagct gcccgccagt
     3901 ggcggcaggc cgtagttaag tacggacgct gccgcgtacg gataacgtgc agcatcaata
     3961 tcatcttcat ggctgacggt gttgagcaga aatgacaaat cccggcggat gatctcctta
     4021 atctgaaccg gactgacgct gataccgtgg tcgcgtttct ggtttggggc gtcgtcacac
     4081 aggcggtcaa acagcgtggg cagcaggtga ttgcctggac gctggcggga agcgctcacg
     4141 cgtcgcttcc ttcagcggtg ttaaaggtac aggtggacaa atccagcagg ctgatatcgc
     4201 cgtgaccggt gagccacacc ctctgaccca gcgcctgtac cagtgtctct cccggaccgt
     4261 cctgccagac cgtttcccgg cacagacgca gggagtcggc agccttttca gagccgctgt
     4321 agcggggaaa cagctaggca ctgtgtgtat cgccattgtt cagggtgacg ttgacgggtt
     4381 tccagatcaa atcgatcaga tgcgcaggcc gcggcgattt cagcgaacag atttgtgaaa
     4441 acggcagcca gatatatgct ccgcccgcga tcagttcgag taccggaccg gtacgggagt
     4501 cgctgtcgct aatccagtca aatgcgccgc cattccactg cccggaagtg tctgttatgg
     4561 cctcaagcgc gcggctgcgg tgagcatcag cttcctgcgc ttcaccacgg gcgttacagg
     4621 cgagggcggt cagcagatcc tccatccagg cgggcggcgg gagaataaca ccgggccgct
     4681 gttcgccctg aaagcaggcg tggcggtata tctcacagcg gatcagctcg ccaaatacct
     4741 gcgcttcgcg agtataattt gcgtccatcc gggcgcatag ctggatctgt tgcagtgccc
     4801 gcgaccagtc gcctgccacg cacaataact gaaacaggct gtggcggcgg gaggcctgcg
     4861 ctgggtgcat cctgacctgt tgctctgcca tctgaatgcc ctccaccacg gaataatccc
     4921 gcagcagcgc cgacaaggta atcggtagct cgttattgtt tgtcatgata tatctctcca
     4981 tgttgtgccg ggtttatccg gtaatggctg tcaatgccaa tgatgcgatg ctctttccgt
     5041 gtcagatctg gcagtacggt gttatgcgct gtttccgggg cgtattccgg ggaaagtaaa
     5101 tgcaggatat cagggggagt cttttcgaca ggccagatag tttcatcttc gccagtggca
     5161 tccagtacat tgagaatagc gtcaatgctt aacccaccag tgaccatatc ctgcaatgtc
     5221 tcggcttcgg tgaccaggtc acgcaatgaa tccagatcct gcgaggaggg ctcttcgtta
     5281 aaaggaaaag gatcagcatg gcgattaaga tcttgttcgg gagataacaa cgcctcgcgg
     5341 tattcctgat aaagtcgggt cagggcattt cctttttccg caccttctcc ggtggaggag
     5401 gctccatgaa caggaatgat atcgaatgga ttttgtgatg tcagatgctg ctgcctgaac
     5461 caatccagat ccagatattc ggttattgct tccgacgtga tggcagtctg cgatatcgtt
     5521 tttactgtgc tcaggttcgg agatgatgga ttgccatgta caaggcacta tgaagataac
     5581 ccccattcta tcaggtcgcc ttcattcaga cgcatacggt gatgaggttc cacaatctgg
     5641 tcgtttaccg tacagcaata ttcggtagac tgattgataa tccaccatgc ttcttcgtgg
     5701 cgggtcagag tgagggcaat ctttacatca tcattcacct taaaaacagg aatccaggga
     5761 gaggtaacgg tgaagataat ttgtgttcct tcagtcgcgt aaattgtgtt atcggcgatc
     5821 ccgtgcgcct gtattttttg gagtccccac gtaatatttg gtttcattgt
//
LOCUS       DAJTSI010000193         5821 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000193 DAJTSI010000000
VERSION     DAJTSI010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5821
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.194"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..392)
                     /locus_tag="OO327_003684"
                     /pseudo
     CDS             complement(1..392)
                     /locus_tag="OO327_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(578..3886)
                     /gene="icsA"
                     /locus_tag="OO327_003685"
                     /gene_synonym="virG"
     CDS             complement(578..3886)
                     /gene="icsA"
                     /locus_tag="OO327_003685"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCR6020063.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4415..5617
                     /gene="virA"
                     /locus_tag="OO327_003686"
     CDS             4415..5617
                     /gene="virA"
                     /locus_tag="OO327_003686"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCR6020064.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
BASE COUNT         1804 a         1321 c          964 g         1732 t
ORIGIN      
        1 caggctgtac gcttccccat gtgatttgcc tcgccacttc accgggctca ggaccccgta
       61 ggatcttcac cgaaaaaaat gcatcaccag ttgctttatt atttgtttta ggtagacctg
      121 ttactaattt atacctactc tgaccaagtc tggataagaa aggcacacct gcacatttct
      181 acttggaaat gttttctttt accattttgc tcaaaaattt caacatccaa tattcctcgt
      241 acagaactac tcaagtcctg aatagaaaaa gttccagctg gtacagtaga gtcatatatt
      301 actcgacctt gttgagatac aacaacacgt gcattagttt ctgcaatacc agtaatctga
      361 ggcgcataac cacgaatatt tggcggtagc attcgttcat cgctttctaa tgcaattcct
      421 gtgtaacgcc aagcaacagc gcattctggc gcatgatctg gtaaacacgt ttggcattga
      481 tcgcaggcat accatcatgt gcacaaaaac ccaccgagct tttacacggt gggtcccaga
      541 gaaatgcagg acatcaacac gccctgcatt tttattatca gaaggtatat ttcacaccca
      601 aaataccttg ggtgtctctg taactgttat tacccaattg ctgagatacg tttccccaga
      661 tgcttaggtt atctgcaaga cgccccccca caccagtttt gagttcaatc acattacggt
      721 tgcctatctg gtgatttgac atgccattca taattacacc atattgcttt gagctccatt
      781 tccaattcac ttccaaaaat ggttcgaact tacgaagagt ctccttatcg atacaactct
      841 gactaattaa ggatgtacgc aatcccaatt ttgcttgaat attattgccc ccagaggatg
      901 tgaccatagt gccattgtgt tcaatatagt cgtttgcttt taccccatgc ctagttatct
      961 ggacttgtgg attgagatac aatgcgttgt caatattatt atgagcagtc cagcgtatgg
     1021 ttgggtagat atagccagct tccaaagctc ctgttattcc tttgctggaa tatttctcac
     1081 cagttaaccc atctcctttg actgtattat taaaccaact gtactgcaac caagcatcca
     1141 tatatagacc tgttcgttct ttttcatcct gaaaccaaga actgtacaaa ccggcagtat
     1201 aaccgctcac agttcctcgt gaactataac tagtatactt attatgagtt ttgctatcct
     1261 gatatccata agctcccata atacccacat gcaagccatc atgatcagtg aagtttgtct
     1321 tcaaaatatc tccaccaatc tgataaacca tcctgttagc tgtggttttt aattgtctat
     1381 cagctaaatt atgctcatta tgtccaccaa cagtacgaat ccacatggtg gtttcgctga
     1441 gctgacctgt agtaggatca acaatagtgc taccgtgtcg gtcatagaga gaaggactaa
     1501 acatcgtgtt cattgctatc aggttagcaa tataactgcc agcttcaggg cgccatgtgt
     1561 gaatactacc ttcacccaaa ttaagtgaag atacaagctg tgaactagct cggcgactac
     1621 tcatttgagt agactcttga ttgctcatct gttttgattc ttgattatcc atctgactag
     1681 ttagatacca cttattggta ttcagtccag atacagtgcc ctgtttcagg cgatagtcat
     1741 aactaccagc aacaatacgg cctttctgaa taaaagcatt cttatcagaa gaatctgtcg
     1801 aaataatttg aacaccttca agagttttat ctccagagcc tccctcattt gtcactgcaa
     1861 cataggtaat accagacgca tggcctgcaa tttccagccg gtcagtttta ctgttgtcat
     1921 tgccgagaac cgcgcccaaa gataatattc cgccctttcc atgccaatta cctttctgaa
     1981 cataggtctg accgacattt gacgaactat tattgagaat aacatgaccg tttttctcca
     2041 gagtcatgtt acctgtgaca atgacggggt caggcaaaaa aggggcatta atattattga
     2101 ttagcacagt cccactattt aataaagtgt taacagtttg gttcatgcct gaaaaattca
     2161 aggtagcacc tttattaaca ataacaccag cggtacgtgt catagcttca actgtcccca
     2221 tttttagaat gcctgcattg atgttggtga aaccagagta ggtatttttt tccgccaaaa
     2281 tgagagttcc ctcccctgat tttgttaggg attttccgtc ccagctattt atattatgat
     2341 ggctgagatt atctgccaga atagtactaa cagtaagttc agcgttttct ttcagattga
     2401 actctccatg actgtcgcca tctgtatcat tccagctcaa accgtagatc aatttattct
     2461 ggtcactgat atagcttttt gtatagtcaa tagcaccagt agtgagtaac ggattgatga
     2521 tagatatatt agaaacactg aaacctgtta tccctttatc tgcacttagc agtttatagt
     2581 ccttctgcgc ccagttattt ataatagcct ccacttttac attgttgcca aatgacatat
     2641 ttccagaagc ttccagacta ctgattccag ctaaatttaa agaactgttc tcaccaaaag
     2701 aaacgtcacc tgaaacagtg aatttgttac ctgatagtga aacagttgta caatcaccag
     2761 cacataaatc accttcaata gttggtgtgg tcccagtgta atcattgttg ataattttca
     2821 gaatggatga attgttaacc tgtacatcac cagtaatgac agaaccttca tggagtatta
     2881 atgaattgtt tccaccagtg atgtggatag cattacctgc tacattttgt gttccatcat
     2941 cttctttacc tgaaattata tatccgctat tagcaatggt catattgcta ccatttatag
     3001 catcccccca atgtccacca ttaccgccta aaatagctcc attgttgatt acagacaggt
     3061 tatctcctgt gatagcatta ccaccgtagc catcataacc ataaccgtaa gcagcacctc
     3121 cgttgcctcc agcgaacgtg cccttattga ttacagagag gctgcttcct gtgatggcat
     3181 cacctccatt accgccatca ccctcaccat aattgttacc tccattacca ccggaaatag
     3241 tccctttgtt gattatgggt aaattgactc ctgtgacggc atcacctcca ttccccccat
     3301 cactatcact ataactatcg ccaccatgtc cacctgaaat aatttctcca tttattataa
     3361 acagattgtc tcctgtaaca gcctcaccac catcaccgtt atggtcagca ccgctaccgc
     3421 cgctaccacc aagaatcatg ccttgattga ttatagacaa gtcagatccg gtaatagagt
     3481 caccaccatt accgccacaa ctattaccat cattattatc accagcacca ccatgacctg
     3541 cgctaatcat aatatcagag ttaatccgta gcttagttct agatgcatga gaggggttgg
     3601 aagaagttat ataccacgca tccattccat cttcaccagc tcccaaggga gaaagtccat
     3661 caacaggtgt taataatttt tcataattgt cctctgaaaa atgaagttct tgagtacccg
     3721 aaagaggagt agcaaaagct attggccccc cgagcaacag ggatgcacca acaacaaaag
     3781 gagaaaaaga caatttgcat gttttttcct ttaccgtagg taattctccg gcccccccct
     3841 gtgaacattg ggtcatatta caaaaaaatt tgtgaatttg attcatgcac tatattatca
     3901 gtaagtggtt gataaacccc tgaaaagatt ccgttttatc cggaataaag ggacgatata
     3961 tgcaaaaaca tattaaacaa agcctcaacc aaaacatgag agctgtgtta tatatcaatg
     4021 caacgggaga aatttacctg tgtcttgctt tgaaaaaaag agggggaaag ctatatgttc
     4081 gataataaat taataatttt actatgatag tctgcttcag accgcaactt ttcagttctt
     4141 tctgtacgct tgcccaaagt ttaaccactt tgtttttcta tacacccaat ggttctatct
     4201 gccccttgat gttaatcccc aaagccaata acatgaatgt attcatatca caactttcct
     4261 accagactct ccaatgcaat aactgcataa ttgtttttag tggaaatgta tcgttttctt
     4321 aaagagaaga ataacattcc attttattat gttcccaatt taattatatt aatatatatt
     4381 ggcattaata ggaaaataca tcaggagaaa tcaaatgcag acatcaaaca taactaacca
     4441 tgaaagaaat gactcatcat ggatgtcaac tgtcaaatca actacagaag taagttggaa
     4501 taaattgagt ttttgtgaca ttttactgaa aataataact ttcggcatat attctccaca
     4561 cgaaactcta gccgaaaaac atagcgaaaa aaaactaatg gatagtttct caccatcact
     4621 atcacaagat aaaatggacg gagagtttgc ccacgcaaac atagatggca tttcaatacg
     4681 gctatgcctt aacaaaggca tatgttcggt tttttatctt gatggagata aaatacaatc
     4741 cactcaactt tcatcaaaag aatataataa cttgttaagt tcactccccc ccaaacaatt
     4801 caacttagga aaagtacata ctattacagc tccggtatca gggaatttta aaactcacaa
     4861 gcctgcacca gaagttattg aaacagccat taattgctgt acatcgataa taccaaacga
     4921 tgattacttc cacgttaaag acactgattt taatagtgtt tggcatgata tttacagaga
     4981 tattcgagca tctgacagca atagtacaaa aatatatttc aacaatattg aaataccact
     5041 aaaattaatt gcagatctaa ttaatgagtt aggaattaat gaattcattg atagcaaaaa
     5101 agagcttcaa atgctaagtt acaaccaagt aaataaaata atcaattcaa acttccccca
     5161 acaagaccta tgttttcaaa cagaaaaact tttgtttact tcactctttc aagaccctgc
     5221 atttatatct gcactaacat ctgcattctg gcaatctctt cacatcacgt cttcctctgt
     5281 ggaacacata tatgcccaaa ttatgtccga aaacatagaa aacaggctta atttcatgcc
     5341 tgaacaacga gttattaaca attgtggaca tattataaaa atcaacgcag ttgtccctaa
     5401 aaacgacact gcaatctctg cctctggggg gagggcttac gaagttagct catcaatatt
     5461 accatcgcac ataacatgta atggtgttgg tattaataaa atagaaacaa gctatctcgt
     5521 tcatgctgga acacttccaa gcagtgaagg gcttcgcaat gctatccctc cagaaagtcg
     5581 tcaagtatca tttgccatca tatctcctga tgtttaagcc aatattatat tggctgccag
     5641 actgtaaaaa aacgttcttc agaacagctc tataaactat taaaccaaag ctaaaaggat
     5701 ggggaccaat agaatctcag cccatccttt ttatatcatt atgaaccgcc ccggaaatcc
     5761 tggagactaa actccctgag aaagaggtaa acaggatgac taaaaataca cgtttttccc
     5821 c
//
LOCUS       DAJTSI010000194         5738 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000194 DAJTSI010000000
VERSION     DAJTSI010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.195"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..241
                     /locus_tag="OO327_003687"
     CDS             <1..241
                     /locus_tag="OO327_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020065.1"
                     /translation="FGIFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPR
                     TVGKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(238..1179)
                     /locus_tag="OO327_003688"
     CDS             complement(238..1179)
                     /locus_tag="OO327_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCR6020066.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRIVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1191..1680)
                     /locus_tag="OO327_003689"
                     /pseudo
     CDS             complement(1191..1680)
                     /locus_tag="OO327_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1684..2904)
                     /locus_tag="OO327_003690"
     CDS             complement(1684..2904)
                     /locus_tag="OO327_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCR6020067.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2908..3651)
                     /locus_tag="OO327_003691"
     CDS             complement(2908..3651)
                     /locus_tag="OO327_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCR6020068.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3542..5008)
                     /locus_tag="OO327_003692"
     CDS             complement(3542..5008)
                     /locus_tag="OO327_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HCR6020069.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(5008..5241)
                     /locus_tag="OO327_003693"
     CDS             complement(5008..5241)
                     /locus_tag="OO327_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR6020070.1"
                     /translation="MDELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAK
                     REIPSPNVADAFIMAFAPIDTSLDIWEQLGRQA"
     gene            complement(5229..5708)
                     /locus_tag="OO327_003694"
     CDS             complement(5229..5708)
                     /locus_tag="OO327_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020071.1"
                     /translation="MDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLTVSEIRLTPLTTENSILWMS"
BASE COUNT         1226 a         1618 c         1435 g         1459 t
ORIGIN      
        1 ctttggcatt ttttacctcg ttacgcgcgt ctgtggcttt gccaacggat acttccgcgt
       61 gcgcacgagt gaaccagtgc gtggcaacgt cttcctccac aacatgacgg cctttaacaa
      121 actcgcgccg agaaccgtcg ggaagcgtga gcacaaacgg ggtatgtacg tgtattactg
      181 catcattttt tgccatcggg tcatccttaa tggccccgcc agggggccat gtggctgtta
      241 aatgccatca acgtacgaaa tggtttcttt gtacactggc tcgactgcac ccagcttgcc
      301 gtagtaagtg acgatctgat acagaccgcg atactgcacc ggcacgctct gaagcggaac
      361 cagcgggtag cggacgtatt ttttatcgtt ggtgtacgca actatgcgat ccttattccc
      421 cacaccacgg cctttcagcc atttaaccgc gcggatattc agcggaacac cgttctggtg
      481 atagctgatg gtgttggtct gaaggttcgt caacagggac tggttacctg cagatgaaac
      541 gatgatgctg gacaacagag caaactgctc aggcgggatc agcaaatcac gcggaaccac
      601 agagtaacca gaagcggccc acgcatcaga cagcacctgg ttaatgcttg cgcggatttc
      661 gtccggtgtt gaggttgccc acgttttggc agcgttgttg acaggcacgc cgtccagggt
      721 aacaaggcct ttcaggttta atgcggaatc gccaacatat acctgttcat cgttatccat
      781 ctgccatttc agttgcatac cgtcatactt ctgcgtatca atcgggcggc cgacctgctg
      841 agcagcctgc aattctatga ccgtccagcc aagttccatc ccccacaggt tcagcgggtt
      901 accggatttg ccgatatcca cgttcacgcc agcaatagcg gttgagtctt tgcctaccca
      961 gttttttcca ttcggatttg cgccagtacc cgcagcggcg aagctggtat tcgtccagct
     1021 ggaaatgtca tctgcgatgg aggcgtcttc acgcagttga atatcgcggc tccaggtgta
     1081 ccccaccagt ggcaggttca gcgtctggtc gagtctctcc agctccccga tgagaaaggc
     1141 accagagctg tcaacggttg cctgatcaaa agtaatcatt cgtctgttcc ttaaatcttc
     1201 caggaaattt ctgcattgcc gtcagcatca ccggcacctg tgaattcagc gttggtcagc
     1261 accacgtttt tgccactgac tgacgtggac atgaatccac ccagaggcac tttgatggat
     1321 tcatcagtgg agatcgcctt ttttgatagt gctggcatca aaatcagaac cgagattaac
     1381 ggtcacgtag ccacgcttca tggcgtcgcc cgggaagttc ttgcctgttc ccacctggcg
     1441 aaccatgtcc ggctgcgacg tggtcggata agggcgcacg tagatcccct tcaccttgtc
     1501 tgcggtatca ccatctgcca gcggcacgaa aaaaccgtca tcatcgtatt taccagccag
     1561 gccataggca gcgaaggcgt tatcggattt aaggaccacc ggttcgacgg ttaagtcctg
     1621 cgggcgagag acagccccgg caatgccaac aggcatccgg tacagaaata cattattcat
     1681 tttttaccct ttacggtttg cccagaattc agcgttttgt ttgttcaggg aagcgatact
     1741 ggtcatgccc atgtttaggc gctgtgcatc gccggtggtg gcgcgggtgt ttcgcccttt
     1801 ggcaacctca gacacggcat taaacgccat gtcgaccgat tgtttcggta atttgcggat
     1861 atccgcatca ccgactatct ggcgaaccag cgttttgtca gcagaagcca gaacctcgcg
     1921 tttgaacgcg gtcggtttca tcttacggct cagatcgata cccggaacga taacttcggc
     1981 acgccaggct gagtcaccag taatcgtggt ttcctcttca tcgtcctcgc cgtcaccggt
     2041 cggattatcg tcaggcttat tatcgttatc gcccgtggca tttctttcca gcttagccag
     2101 cagggctttc agtaatgttt tgaggtcatc atcactgtcg ccggttggat ctccgcccat
     2161 ctctggtgct ttgtccggta gtggttgctg cggggacagg ttgatgttga gattaacgcc
     2221 ctgcggcaaa tccccctcat ctcctgtaac cgatgcggga gccgactcca ccagttcgtt
     2281 catggtgtcg gcatctcctg tcttgatggc cgcacgcatg cggttccacc agtttttctt
     2341 ttgatttgcc attgtgtctc tgtctccaat tgcacaacga tttccggctc tgcctttagg
     2401 gacaagagcc acatggtttc cggtaatatc gacctgctcg gctttacctg gctcggtctg
     2461 ctcgtactcc gcgtcatagc cgcacgacac ttcgcgcagg ccatcttcga taagctgaat
     2521 ggcgctttcg tctttgacga taaggtcagc cagcatcaaa tcagactgct cacccgtccc
     2581 gcgccggaca ttctggaggt gcccgacagc aagcactttc cagttctcgg gatttaccag
     2641 ccgcacattc ccgttttcat cttcaggatg caggatcgtg atgctcatcc cttcgaatga
     2701 ggcaagcgtg gccggatgga atacctgctc aggagaacgc gtgacgacta tttcaacgaa
     2761 cttatcgggt ttcagttttg gcaggtcatc agcaccatag agctgcttac ctgttcgtcc
     2821 tatcggcacg tctttgcaca gcaacgagcc gtcagccagg cggtatcggg tttccccgag
     2881 cctggtctca aaaaagtatt tcatgggtta tgctgccctt ttcattgcga atggaacgcg
     2941 aacaaacacg atctctttat agcagcggca gtttggcagc tcgcccgcgt ggccggtcat
     3001 gccgtccaaa gttggaggtg aatcccagcg gacgaattta cctttcatgt gatcgtggga
     3061 atctcggaca tcgccatcat cggctgtacg ccaaatataa ccatccgagc caatagctat
     3121 cgctcgggcc atatccagcg cgcccgttgc tcttcccagt tccgttcggg caatcagatc
     3181 ggccctagac ttagcaacat cgccggtaga tgcaatctct ttagcaaact gctccgcccg
     3241 accaccggtg acaacagcct ctatcgcttt gttctggatg tcgtacaccc tgtctgcggc
     3301 ctcgagaggc agagatttga tgtacttgac ctgttcggcg atgatggatt gcatcacctg
     3361 gcccacagga gcgctttcca caagattgcg gagctcgcga ctgatgttct tgctgtgttg
     3421 ccgccaaact ttctcgttct gccgggttag gtccgcagta aagttttccg cgacctttgt
     3481 cgcccagggg gtgatgattt cactgtaacg ctccagcgcc gcaataattt ccgtgatact
     3541 gtcatttgaa ccatcgtagc gaccatttac gatgtctccg accgccctcg ctatcctgcg
     3601 taggctggtt cgataccgga tttccgcctg acggctcctg cggttcgtca tcaggttcgc
     3661 cgatgccggg cggcgcttca tcttcggcat tctcgatgtc ctcgttggta atggatgccc
     3721 ctatgccggt tacgtcagaa ttttctcgca aatcagtcat agcggctttc agtgtcatca
     3781 gaccatcacc cagcgccgta ctgattgcgt tggtggtgtt taacgccacc gttgagcgat
     3841 cgacatcaga catttgccag agcgggttaa actcaaacgt gaaatcgtcc ggcagcggct
     3901 tgccaagttc cgaacgatgc atgatgtcca gtatccgccg caccggaaga cgtaaacgcc
     3961 tctcctgcaa cgaactgatg cgatcgtaat agttggcaag gtctgcatca ccggtagaaa
     4021 atcctttcgg ggactgtccg aacaaccgca ccagtgggat accaacagcg ccactaatct
     4081 gttctgcaaa ctgcgaaagg atgtcatcca gaccactgaa gctgtactga tgggtttcaa
     4141 acttatcccg cgagtccatg agcgtcatgc cttcattact ctggaactgt cgaatcaggt
     4201 cgatattctt cagcaacgct tcatacgcag gaccaccaag tgcgataagc tcgcgtagct
     4261 tctccacgct gtaggtacgc aaatgcgcct tgtagaccag ctgcgccgcg ccgacagtag
     4321 cgctgtcgaa cgcggtaaga cgatcccaga tacgctctac aaccgacatt ccccattcgt
     4381 tctcggtcat cttctgctga aatggcaacg tgacgccatc aaagcgaatc aggcgactgt
     4441 gatgaatgcg ccaggcagga attcccgttg ctgtggtcac cacatcgtaa aactcaggtt
     4501 tacccaggtc cggccccata tctttaatgc ggcgggtcag tgccgggtcg accatccagc
     4561 ggtcgagcgg gagaatcccc ttaaacttgc ccttaccgat ggtttcgggt cgcagcgggg
     4621 tcattggtgc ctgcccctca atcatgatga aacccaccgc gccgccgtag aggcgcgacc
     4681 atttcagcac gtcattcagc gcatcccaga tttgcaactc atccagttgt gattcgagaa
     4741 tgccacgatc ttttgcatca atttccgaag tgatgcgaat gcctttgcgg gtcatatcat
     4801 ccgggatagc atcgactgct tcgccgatga tccaggatga acgataggac cattccacca
     4861 gcatgcggtt acgactggtg aaattagccc ggtaggtgga tgctgagtgc tggttaggtg
     4921 tctgcatccc tactcgggca ataaaattct cataaccatc agctgtggcc tgcacagttc
     4981 gccgcagggc ttgtttgttt cgtgccatca ggcctgtctc cccagctgtt cccagatatc
     5041 cagcgatgta tcgattggcg cgaaggccat aatgaatgca tcagcaacgt ttggtgacgg
     5101 tatctcgcgt tttgcgaggt cttttttact ttcgaccatc acacgtccgt tacggtcgaa
     5161 atcacgatga ggtgttgtca gttccagttt cagcttttca agcaacggac aacgagaatc
     5221 tatgctgatc agctcatcca caggatactg ttctccgttg ttaatggcgt taaacgtatt
     5281 tctgaaacgg tcagccacca tgcctgagcc ttaagatttg cgaaaaagtc tttgttgggg
     5341 atgccgttgt actcgtcatc tggctcatgc acaccagcac cggcgttaaa cctctggtaa
     5401 ttcacacgtc gcgcgtatgc gttctcgctc ttccggtcag cgttaatttc agagaattta
     5461 gcaccggcag acgcaccaac accgatagag tcgtaaacaa tatctgcttc acgctccagc
     5521 gccgcctgat aagtacgctg gcagctcttc agtaattcat cttctttggc cttccattcg
     5581 tcggcccaga aaacaacgga tccgtgacgg taaacgttag cgcacttatc tgtaccactg
     5641 tcagccacgt caaagccaat acgctttctt ccactgggtt cgaaatttaa cgttttgtgc
     5701 gcatccactg cggcttctat ccaagacagt ttgatgat
//
LOCUS       DAJTSI010000195         5711 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000195 DAJTSI010000000
VERSION     DAJTSI010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5711
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.196"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..140
                     /locus_tag="OO327_003695"
                     /pseudo
     CDS             1..140
                     /locus_tag="OO327_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            665..1843
                     /gene="ltrA"
                     /locus_tag="OO327_003696"
     CDS             665..1843
                     /gene="ltrA"
                     /locus_tag="OO327_003696"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR6020072.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     NGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            1926..2105
                     /locus_tag="OO327_003697"
                     /pseudo
     CDS             1926..2105
                     /locus_tag="OO327_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2135..2617)
                     /locus_tag="OO327_003698"
                     /pseudo
     CDS             complement(2135..2617)
                     /locus_tag="OO327_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2873..3853)
                     /locus_tag="OO327_003699"
     CDS             complement(2873..3853)
                     /locus_tag="OO327_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR6020073.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3853..5052)
                     /locus_tag="OO327_003700"
     CDS             complement(3853..5052)
                     /locus_tag="OO327_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6020074.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5374..>5711)
                     /locus_tag="OO327_003701"
     CDS             complement(5374..>5711)
                     /locus_tag="OO327_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6020075.1"
                     /translation="HWLWYAYNTKTGGVLAYTFGPRNDETCRELLALLTPFCIGMVTS
                     DDWGSYAREVPKEKHLTGKIFTQRIARNNRTLRTRIKRLARKTICFSRSVEIHEKVIG
                     SFIEKHMFY"
BASE COUNT         1567 a         1308 c         1360 g         1476 t
ORIGIN      
        1 tgcgccagta tgtgctgatg cccggtaaag tccatgccga tgatatcccc gttccggtcc
       61 aggagccggg cagcggtaaa acaaggactg cccggctgtg ggtctacgtt cgtgatgacc
      121 gtaacgccgg ttcggaaatt gcagcataag ttatccatgc tctgaggtgg gtagaggtgg
      181 gccctccggt cagggtttac aggagcacag accaaacgac ccggtgctgc ttcattctaa
      241 ggaatgaggt ggtgagcgac tcgggaaaag gcagagatct actgtcacgt gggttcggga
      301 agctgaacga aagtaaactg tccgatgaag catcgaaatc ttagatgatg ccaaaatggg
      361 gccgtacagc cgtcccctga taagcgcaga agacacctgc ttactgactg cgcggcatcc
      421 ggtgtgaagg cagcaggatc ccgggccaga ccatctcagg gaacgtgaga acctgtttcc
      481 ggcacgtatc gggaaacagg ggcggaccca ttcgtagtag tgatgaaaga tctgtaatgg
      541 gtctggagcg aagggatggg gatcacattc attgcagaag acacaaccca aaaggggatg
      601 acgtaactga tgcaacgaaa atcatttgaa ataccaaaag cactggtctg ggcatcatat
      661 ctggatgtgc gtcgcaacaa gggagccccc cggatgcgac gggcagactt tgaaaatgtt
      721 cgaccagcaa cgtgatggca atctatacaa gatctggaac cggctatgtt cgggaacatg
      781 gtttccgcca ccagtgctgg aaaaacggat cccgaagtcg aatggcaagg aacgcattct
      841 ggggatccca acagtatccg atcgaattgc tcagggagcg ataaaacttt tcatggaaga
      901 aaagcttgac ccgattttcc atgctgattc atatggttac cgaccaggca aatcagctca
      961 tgacgctctg aaacaatgtg ccatccggtg ctggcgttat agctggatac tggaagtgga
     1021 catcagtgcg tttttcgacc atgtgagaca tgatctggtg ctcaaagctc tggaacacca
     1081 cgggatgcct aaatgggtca tcctgtactg tcgacgctgg atggaagcgc caatgcagag
     1141 ttgtgaaaat ggagaattaa taacccgaac acgaggcact ccgcagggcg gggtcattag
     1201 tccgctactt gctaacttgt tccttcacta tgcatttgat ttgtggatgg aaagagaata
     1261 tcggggggta ccgtttgaga ggtacgctga cgatattgta gtacattgtt cacgaatgag
     1321 tgatgcaaca aggctaaaga acaggttatc ggagcgcttc tcggaagtcg ggctggtcct
     1381 gaatgcaggg aaaacgaata tcgcctacat tgacacgttt aaaaggagaa acgtcgcaac
     1441 gagtttcacc tttctcggat atgacttcaa agtgcgtacg ctgaagaatt tcaacggcga
     1501 acggtaccga aaatgcatgc cgggtgcgtc aaatgcagca atgcgcaaaa taacagaaac
     1561 aatcaagaag tggcgtatac atcgctcaac agctgagagt ttgctggatt ttgcgaggcg
     1621 ctacaatgcg atagtgagag gctggatcga gtactacgga aagttctggt ccagaaattt
     1681 caactatcga ctgtggagtg caatgcagtc acgtctgctc aagtggatgc agtctaaata
     1741 cagactttcg aaccggaagg ctcagcgaaa gctgacgctg gtaaggaagg agtatccgaa
     1801 gctatttgtt cactggtatt tactacgtgc atcaaatgag tgatcaagag ccgtatgacg
     1861 cgagagtgtc acgtacggtt ccgtgagcag ccggggagtg aaattctctc cggctgactc
     1921 gactcgcggg ttccgacagc gatgacgaac atgcggcggt gttgtactcg ctgatcggca
     1981 catgccgtct gaacaatgtg gaaccagaaa aatggctgcg ttacgtcatt gagcatatcc
     2041 aggactggcc ggcaaaccgg gtacgtgatc tgttgccctg gaaagttgat ctgagctctc
     2101 agtaaatatc aatacggttc tgacgagccg cttacttttg gcgtaattct gctgcgcctt
     2161 tactgaacac aatgccgtat tcagcaagaa gtccacgaat gtgatttatc agttgtgtcc
     2221 tttccattga aaccaaccga cgcatattca aaaaagtctg gacgtcctgt tgctccagag
     2281 tttttattgg tacgggtcgt atagttccat gttggcaggc ctcagctatt gcctgtgcgt
     2341 cgttataatc atttttctgg caccgctgat aagctttaac atgttgtgcc ggaagcaata
     2401 ttacatcatg gtcgagtttc ctgatttccc gggcccagta atgagatgcc ccgccagcct
     2461 ccatcgcaac agtcatttta ggctgttgca ccagccagtt aagcagttga ttgcgagaaa
     2521 gtttggtctt tcctgcgggt ttgccatgag cattgatact gaaaagataa aagttcgttt
     2581 tagccagatc aatgccggtg attttctgtt cagacatggt atcccgctta cttttgttgt
     2641 taaagtttat ccatcatggc gcaattgaag gctgccgtga aagaggggga agactatctc
     2701 atcggttact gttaaacggc taagagaaac tatctttttc aagcaaagaa gatacggtga
     2761 aacaggcgat ttcaccgtga aatactgttc aaaaaaaacg ctaataaaag caaaatttct
     2821 tttaaaacag ccaattaaaa actggcgagt tgctggcgaa aaacttgaat tttcacagcg
     2881 aattcagatt atcttttagt acaagagcaa tcatacgatc taacttatcc tgaacttcca
     2941 acggtaatct gccgaattca tatgagaacg tacgtccctt tactcgtttt cgagcaaatt
     3001 tatctttact atcaaatttc cataacaaag ttacaaccgc tttatccttt actcctgaat
     3061 caagcaatga agacatttcc ttggttataa gccgcataat actgttttta acctcgtctt
     3121 cagaaagatt gtcatcagca gtaataagct caatactcgg cgagatattc ttcacaactt
     3181 catcaataga tatatcaccc agcctcagtc gttcacttaa ttcagccagt tgcctgtaat
     3241 cggcgtatgt cagctcagac tgaacaggaa aaagagacac aaaatcttta ggtacggacg
     3301 ctgcttggag ggcacgagtc accttagctg ccgacaatcc ctctctttcc gcaatttgct
     3361 tctggttcat accggcctct ttgagacgta ctaaacgcaa cccaatttca cgaatattat
     3421 gttcaagtga cgtctgcaaa tctttcgcca gatgctgagc ttcagaaaca gtaagctcat
     3481 catccgttac aagtacacgt aatccaacct tgcatagcaa agctgctgcc cgacgacggg
     3541 aaccatccaa aatctcaata agatcgccag tccgaatccc aatacatgga tagaattgtt
     3601 gtaaacgtat agttctggtg atgtctttta gtgagtccgg cgtcagggct gtctgttcgc
     3661 ggccattcac ctcttcaacc acaaaagttt gagtatccac tttgtcatga tcaaccttga
     3721 tctcagtaaa ttttgccttt cggccagtat tgagggtaaa tacatgaact ggagccgata
     3781 tttcttcagt attattcagg atatttgtat tcagcgtacg gccaatagtc ggtcggtgct
     3841 ttctgttttc catcagtagt tggcccttat aaattcaata cgatcaaaaa ctgctttagc
     3901 gaaatcttct gctgccatac gtgcattttt caaagcttca ccactgccaa cataagtaac
     3961 aggattcgca gatatcacgg tatcaaacga ctcgccgctt ctctcaaaac catcaagacg
     4021 tggcattgaa acatcaagca tgtcaccacc gaaaatctct tttgtcaggc tatggcagta
     4081 tttatgatct gacttattcg ccagtttcga catgaagcca atatttgcct gcaaacggca
     4141 tgaacatcca tcctgttcaa taatctgtac aagttcaggt aaacgagcca aatatttaag
     4201 cgtggaatgg aaatcaactt gtgctggcgg caccggtgta aacatgatat ctgcggcagc
     4261 aatggcattt ttcaaaaatg cgtccagatg cgggcctgta tcaataagga tgaagtcgaa
     4321 atcatgcttc agcttatcaa taatattctc acgcaatatc gcgtgtttgt tttgtcctaa
     4381 tagatgctcc tcgcacagag tatcccagtt tgatgcaata aaggcgtcgt caatagaagc
     4441 cggcataaca taaacgccag ggataactga cggaacgata aaatcttcca gtaattcctc
     4501 acgactcacg ttctgcaaca tagcctgcgg tgcggttgta tcaaccagcc ccaccgcatg
     4561 caagtagttc agaaacatag tggctgatga ctggggatct aaatcaagga gcagaatacg
     4621 taaatcttct gacagcaggt gaggatgagc acgcagggcg tgagctacgg aaacactact
     4681 cacggtttta gacacaccgc cttttagact accgatgaat atggaatatg cctcactata
     4741 acgatcgcga tatttaggaa tgccacgatg tgcatagata tcgataatgt tctggatggt
     4801 taatgcatat tttgtcgcgg ttcctgctgg gcgtttttca aactgatagc cctgagcctc
     4861 catctcaccg actgcaccat ccacgcttgc gcgcgttaat tttggaagct tagctacagc
     4921 agactttgaa tagacttgat aaaaaacatc agcctgaaac tcctgctttt gctcctgaat
     4981 ctgttttgtt agggcgagca acattttatc tgcacgctgc gcaaccttac ttaactgctc
     5041 aaaactagtc attccccctc cgtgctatgt aggattttaa cttattacaa aaaaaactgc
     5101 attagcaggg taatgcatgc aagtacatat tgtacatgct tatattaaaa atgcgcgcat
     5161 tgctgattta ttaatcatcg gaatgaatat tactgtaaga tagtatgcct ttccgggaga
     5221 aagacgctga tttcccccga ataatccagt tcaccaccac actccggaca acagctttct
     5281 tcaggctcca gatggtgtat ttcgcgggaa acatgttccg gtaacggacg gcgatgacgt
     5341 gactgtcgca acaggcgggg gtagtgcttc caatcagtag aacatgtgct tttcaatgaa
     5401 ggagccgatg actttttcgt ggatttccac ggaacgcgag aagcagattg ttttacgtgc
     5461 cagccgcttg atgcgggtgc ggagggtccg gttgttacgt gcaatacgtt gcgtgaagat
     5521 tttgccggtc aggtgtttct ccttcggcac ttctctggcg tagctgcccc agtcatcgct
     5581 ggtcaccatg ccgatacaga acggagtcaa cagggctaac agttcccgac aggtctcatc
     5641 attccgtggc ccgaaagtgt aagccaacac accgccagtt ttagtgttgt acgcatacca
     5701 gagccagtgt t
//
LOCUS       DAJTSI010000196         5702 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000196 DAJTSI010000000
VERSION     DAJTSI010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.197"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..170)
                     /locus_tag="OO327_003702"
     CDS             complement(<1..170)
                     /locus_tag="OO327_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020076.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
     gene            246..2807
                     /gene="mutS"
                     /locus_tag="OO327_003703"
     CDS             246..2807
                     /gene="mutS"
                     /locus_tag="OO327_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR6020077.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2913..3565
                     /gene="pphB"
                     /locus_tag="OO327_003704"
                     /pseudo
     CDS             2913..3565
                     /gene="pphB"
                     /locus_tag="OO327_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3616..4383)
                     /locus_tag="OO327_003705"
     CDS             complement(3616..4383)
                     /locus_tag="OO327_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6020078.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4579..5487
                     /locus_tag="OO327_003706"
                     /pseudo
     CDS             4579..5487
                     /locus_tag="OO327_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            5484..>5702
                     /locus_tag="OO327_003707"
     CDS             5484..>5702
                     /locus_tag="OO327_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCR6020079.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
BASE COUNT         1410 a         1469 c         1481 g         1342 t
ORIGIN      
        1 atcttaaagg gcactatgac tctaaagaag aagggttcgc caatcgatac aattttgcgt
       61 acttgcttca taagcatcac gcaaaagctg caaaacagca tctttcccgg aaccagcatc
      121 aagaactcgc cgttcgcttc ttcccctgaa atgattaact ccggtatcat gtgcgcctta
      181 tgtgattaca acgaaaataa aaaccatcac accccattta atatcaggga accggacata
      241 accccatgag tgcaatagaa aatttcgacg cccatacgcc catgatgcag cagtatctca
      301 agctgaaagc ccagcatccc gagatcctgc tgttttaccg gatgggtgat ttttatgaac
      361 tgttttatga cgacgcaaaa cgcgcgtcgc aactgctgga tatttcactg accaaacgcg
      421 gtgcttcggc gggagagccg atcccgatgg cggggattcc ctaccatgcg gtggaaaact
      481 atctcgccaa actggtgaat cagggagagt ccgttgccat ctgcgaacaa attggcgatc
      541 cggcgaccag caaaggtccg gttgagcgca aagttgtgcg tatcgttacg ccaggcacca
      601 tcagcgatga agccctgtta caggagcgtc aggacaacct gctggcggct atctggcagg
      661 acagcaaagg tttcggctac gcgacgctgg atatcagctc cgggcgtttt cgcctgagcg
      721 aaccggccga ccgcgaaacg atggcggcag agctgcaacg ctctaatccg gcggaactgc
      781 tgtatgcaga agatttcgcc gagatgtcgc tgattgaagg ccgtcgcggc ctgcgccgtc
      841 gcccgctgtg ggagtttgaa atcgacaccg cgcgccagca gttgaatctg caatttggca
      901 cccgcgatct ggtcggtttt ggcgtcgaga acgcgccgcg cggactttgt gctgccggtt
      961 gtctgttgca gtatgcgaaa gatacccaac gcacgaccct gccgcatatt cgttctatca
     1021 ccatggaacg tgagcaggac agcatcatta tggatgccgc gacacgtcgt aacctggaaa
     1081 ttactcagaa cctggcgggc ggtgcggaaa atacgctggc ttctgtgctc gactgcaccg
     1141 tcacgccgat gggtagtcgt atgctgaaac gctggctgca tatgccagtg cgcgataccc
     1201 gcgtgttgct tgagcgccag caaactattg gcgcattgca ggatttcacc gccgagttgc
     1261 agccggtact gcgtcaggtc ggcgacctgg aacgtattct ggcacgtctg gctttacgaa
     1321 ctgctcgccc acgcgatctg gcccgtatgc gccacgcttt ccagcaactg ccggagctgc
     1381 gtgcgcagtt agaaactgtc gatagtgcac cggtacaggc gctacgtgag aagatgggcg
     1441 agtttgccga gctgcgcgat ctgctggagc gagcaatcat cgacacaccg ccggtgctgg
     1501 tacgcgacgg tggtgttatc gcatcgggct ataacgaaga gctggatgag tggcgcgcgc
     1561 tggctgacgg cgcgaccgat tatctggagc gtctggaagt ccgcgagcgt gaacgtaccg
     1621 gcctggacac gctaaaagtt ggctttaatg cggtgcacgg ctactacatt caaatcagcc
     1681 gtgggcaaag ccatctggca cccatcaact acatgcgtcg ccagacgctg aaaaacgccg
     1741 agcgctacat cattccagag ctaaaagagt acgaagataa agttctcacc tcaaaaggca
     1801 aagcactggc actggaaaaa cagctttatg aagagctgtt cgacctgctg ttgccgcatc
     1861 tggaagcgtt gcaacagagc gcgagcgcgc tggcggaact cgacgtgctg gttaacctgg
     1921 cggaacgggc ctataccctg aactacacct gcccgacctt cattgataaa ccgggcattc
     1981 gcattaccga aggtcgccat ccggtagttg aacaagtact gaatgagcca tttatcgcta
     2041 acccgctgaa tctgtcgccg cagcgccgta tgttgattat taccggcccg aacatgggcg
     2101 gtaaaagtac ctatatgcgc cagaccgcac tgattgcgct gatggcctac atcggcagct
     2161 atgtaccggc acaaaaagtc gagattggac ctatcgatcg catctttacc cgcgtaggcg
     2221 cggcagatga cctggcttcc gggcgctcaa cctttatggt ggagatgact gaaaccgcca
     2281 atattttaca taacgccacc gaatacagtc tggtgttaat ggatgagatc gggcgtggaa
     2341 cgtccaccta cgatggtctg tcgctggcgt gggcgtgcgc agaaaatctg gcgaataaga
     2401 ttaaggcatt gacgctgttt gccacccact atttcgagct gacccagcta ccggagaaaa
     2461 tggaaggcgt cgccaacgta catctcgatg cgctggagca cggcgacact atcgccttta
     2521 tgcacagcgt gcaggacggc gcggcgagca aaagctacgg tctggcggtt gctgctctgg
     2581 cgggcgtacc gaaagaggtt attaagcgcg cacggcaaaa actgcgtgag ctggaaagca
     2641 tttcgccgaa cgccgccgcg acgcaagtgg atggtacgca gatgtctttg ctgtcagtgc
     2701 cagaagaaac ttcgcctgcg gtcgaagcac tggaaaatct cgacccggat tcactgactc
     2761 cgcgtcaggc gctggagtgg atttatcgct tgaagagtct ggtgtaataa caattcccga
     2821 tagtcttttg ctatcgggaa tattaacgac aactgacgaa tcaaataaaa ataccctgta
     2881 taataggaaa gcttatttta cagggtaaaa ccatgccatc tacacgctac caaaaaatca
     2941 atgcccatca ctatcgccat atatgggtcg ttggtgatat tcatggtgaa tatcagttat
     3001 tacaatcccg cttacatcaa ctctcttttt tccccgaaac cgacttactt atttctgtcg
     3061 gcgataatat tgatcgtggg ccggagagtc ttgacgtcct gcgcctgcta aaccagccct
     3121 ggtttatctc cgttaaaggc aaccacgaag caatggcgct ggatgcgttc gcgaccggcg
     3181 atggcaatat gtggcttgcc agcggtggtg actggttttt cgatttaaat gattgcaaca
     3241 agaggcaata gttctgttgc tgaaattcca tcaccttcca catattattg aaatcactaa
     3301 cgacataata aaatatgtca tcgcacatgc agattatccg ggtgatgaat atctctttgg
     3361 caaagaaata gcggagagcg aattactctg gcctgtttat cgtgtgcaga aatcgcttaa
     3421 tggcgagtta caacaaataa acggcgctga ttattttata tttggacata tgatgtttga
     3481 taacattcag acgttcgcta accagattta tattgatacc ggatcgccga aaagtgggcg
     3541 gctgtcattt tataaaataa ggtagtctca tgcttcttct gtgaagcatg agtaaccaga
     3601 tgttatttca ggccattatt catttttcgc taccagcaaa gagagatcct gcttcgccag
     3661 cgcgcgactg gcactctccg gcaaaccgtc gtctgtaata atctgatcaa actcgcttaa
     3721 tggtaacgcc agccatgtcg ccacctgacc atatttcgtc gcatcacaga ccaaaactcg
     3781 ctggcggctg gcactggcaa tcgcccgttt caccgtgact ttatcttccg ctggcgtaga
     3841 aatcccccgc acactccatg acgatgcaga aataaaagcc tgatcaatca tcaggctgca
     3901 cagcatggtc gcagcggctt ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc
     3961 agtgtgaata attgtgcaat tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt
     4021 tgtgaccaca gtcagtgact ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt
     4081 tcccgcatcc agatagatac aacttcccgg cagaacaaga cttgccgcca gcttgccaat
     4141 agccgctttt tgcgtcattg ccagcgcagt ttttacctga tgagaaggtt catgcgccac
     4201 gcgtcccgga gactggacgc ctccggacac cagcacaacg gctccctgct gctccagttt
     4261 ttgtaaatcc cgacgaatgg tcatatgtga cacattaatt ctgtccgtta gttcagcaat
     4321 actgacaatg cctttttcag ctaccatctc aaggatgatt tggcgacgct ctacgggtat
     4381 caacttttgc tccttccttt gtcctgctga cattctacgc tatttgcctg cgaaacgtgc
     4441 gcggcgcaac taacgcttag ttcacataaa ataacacaaa atgttaattt atgtgaatca
     4501 tctcaccata tcgttatctc ccagcgctta tgttcacaat atcaaacaaa atatcactta
     4561 aattaacaag gagagcagat gaaaacggga tctgagtttc atgtcggtat cgttggctta
     4621 gggtcaatgg gaatgggagc agcactgtca tgtgtccgcg caggtttttc tacctggggc
     4681 gcagacctga acagcaatgc ctgcgctacg ttgaaagagg caggtgcttg cggggtttct
     4741 gataacgccg cgacgtttgc cgaaaaactg gacgctctgc tggtgctggt ggtcaatgcg
     4801 gcctaggtta aacaggtgct gtttggtgaa acaggcgttg cacaacatct gaaacccggt
     4861 acggcagtaa tggtttcttc cactatcgct agtgctgatg cgcaagaaat tgctaccgct
     4921 ctggctggat tcgatctgga aatgctggat gcgccagttt ctggtggtgc agtaaaagcc
     4981 gctaacggtg aaatgactgt catggcctcc ggtagcgata ttgcctttga acgactggta
     5041 cccgtgctgg aagccgttgc cggaaaagtt tatcgcatag gtgcagaacc gggactaggt
     5101 tcgaccgtaa aaattattca ccagttgtta gcgggcgtac atattgctgc cggagccgaa
     5161 gcgatggcac ttgcagcccg tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc
     5221 aatgccgccg gaaattcctg gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat
     5281 tacaccccgc attcagccgt cgatattttt gttaaggatc ttggtctggt tgccgataca
     5341 gccaaagccc tgcacttccc gctgccattg gcctcaacag cattgaatat gttcaccagc
     5401 gccagtaacg cgggttacgg gaaagaagac gatagcgcag ttatcaagat tttctctggc
     5461 atcactctac cgggagcgaa atcatgatca agattggcgt tatcgccgat gattttaccg
     5521 gcgcgacgga tatcgccagt tttctggtgg aaaacggtct accaacggta caaattaacg
     5581 gtgttccaac aggtaaaatg ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc
     5641 gctcctgtcc ggtggttgaa gccacacagc aatcgctggc ggctctgagc tggttgcaac
     5701 ag
//
LOCUS       DAJTSI010000197         5644 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000197 DAJTSI010000000
VERSION     DAJTSI010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.198"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            86..862
                     /gene="exuR"
                     /locus_tag="OO327_003708"
     CDS             86..862
                     /gene="exuR"
                     /locus_tag="OO327_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR6020080.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1207..1869
                     /locus_tag="OO327_003709"
     CDS             1207..1869
                     /locus_tag="OO327_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6020081.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1873..2256
                     /gene="mzrA"
                     /locus_tag="OO327_003710"
     CDS             1873..2256
                     /gene="mzrA"
                     /locus_tag="OO327_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR6020082.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2403..2757
                     /locus_tag="OO327_003711"
                     /pseudo
     CDS             2403..2757
                     /locus_tag="OO327_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2795..3100
                     /locus_tag="OO327_003712"
     CDS             2795..3100
                     /locus_tag="OO327_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR6020083.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3103..3507
                     /locus_tag="OO327_003713"
     CDS             3103..3507
                     /locus_tag="OO327_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR6020084.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3497..3796
                     /locus_tag="OO327_003714"
     CDS             3497..3796
                     /locus_tag="OO327_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR6020085.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDISASSREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3982..4278
                     /locus_tag="OO327_003715"
     CDS             3982..4278
                     /locus_tag="OO327_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6020086.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            4348..5334
                     /gene="yqjG"
                     /locus_tag="OO327_003716"
     CDS             4348..5334
                     /gene="yqjG"
                     /locus_tag="OO327_003716"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR6020087.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
BASE COUNT         1441 a         1378 c         1415 g         1410 t
ORIGIN      
        1 tcatcaaatt tatatatgac caatggcata cccatgaata aagtggtatg ataacttcgt
       61 ccgcattcca ctccgggaga cgcatatgga aatcactgaa ccacgccgtt tgtatcaaca
      121 acttgctgct gacctgaaag agcgcattga acagggcgtc tatctggtgg gtgataaact
      181 gcctgccgaa cgctttattg ccgatgaaaa gaacgtcagc cgaacggtag ttcgtgaagc
      241 catcatcatg ctggaagttg aaggctatgt ggaggtgcgt aaaggttcag gtattcatgt
      301 ggtttccaac cagccgcgtc atcagcaggc ggctgacaat aatatggaat tcgccaatta
      361 cggtccgttt gagttgcttc aggctcgcca gctcatcgaa agtaatattg ccgagttcgc
      421 ggcaactcag gtaacgaaac aagacatcat gaaactgatg gccattcagg aacaggcgcg
      481 cggcgaacaa tgcttccgtg attccgagtg ggatttacag ttccacattc aggtagccct
      541 ggcgacgcag aactccgccc tggcggccat cgttgaaaaa atgtggaccc agcgtagcca
      601 taacccgtac tggaaaaaac tacacgaaca cattgattcc cgtaccgttg ataactggtg
      661 tgatgaccac gatcaaattc tcaaggcgtt aattcgcaaa gatcctcatg ctgctaagct
      721 ggcaatgtgg cagcatctgg aaaacaccaa aatcatgtta tttaacgaaa ccagcgacga
      781 tttcgagttc aatgccgacc gctatctgtt cgccgaaaac ccggtagtgc atctcgatac
      841 ggccaccagc ggcagcaaat gaaattcctg tccgacaggc gctgcaatgc gcctgtttgt
      901 ctcgtcggta agcgaaaagt ataaagtgtc agcctgtgta aatcctctcg ccaccctccc
      961 ctgcaatcag caaaatcaga ctccacggac atgtaatttt gataacgaac aacgttgacc
     1021 tttgttacaa ttagattcaa tttgaattta tgcttttgaa tgctttctta tctcacgttt
     1081 taacagggaa tagttcaggc tgtgttgatg tatcaaaccc gcagaacata ccaaaacagc
     1141 aataacattg cggtagtgca tcttttaaaa ccagcgtggc gttaaccgat tcaccaggaa
     1201 taatgaatgg aacttttgac ccaattgctg caagccctgt gggcgcagga ttttgaaacc
     1261 ctggccaatc catcgatgat tggcatgttg tattttgtct tgtttgtaat tttgttcctt
     1321 gaaaatggct tgcttccggc ggccttttta ccgggcgaca gtttactggt attggtcggc
     1381 gtgttgattg cgaaaggcgc gatgggttac ccgcaaacga ttctgctgct gaccgttgcc
     1441 gccagcctcg gttgctgggt cagctatatt caggggcgat ggctgggcaa tacccgcacc
     1501 gtacaaaact ggctatctca tttacccgcg cattatcatc aacgcgcaca ccatcttttt
     1561 cataaacacg gtttatcggc gctgttaatt ggtcgcttta ttgcatttgt cagaacactg
     1621 ctgccgacga ttgccgggtt atcagggctg aataacgcgc gctttcagtt cttcaactgg
     1681 atgagcggtc tgctgtgggt attgatcctg acaactctgg gttacatgct cggcaaaacg
     1741 ccggtatttt taaagtacga ggaccagctg atgtcatgcc tgatgctgct cccggtggtg
     1801 ctgctggtgt ttggcctggc aggttctctg gtcgtgttat ggaaaaagaa atatggaaat
     1861 cgggggtaag ggatgcaaat acctcgcatg tcgcttcgcc agctagcctg gtccggcact
     1921 gttttacttc tggtcggcac gctgttgctg gcctggtccg cggttcgcca gcaagagtct
     1981 acgctggcga ttcgtgccgt tcatcaaggc acaacgatgc cagacggttt ttcgatctgg
     2041 catcaccttg acgctcatgg cattcctttc aaaagtatca cccccaaaaa cgacactctg
     2101 ttaattacat ttgactccag cgaccagagc gccgccgcaa aagcggtcct cgacagaaca
     2161 ttgccccatg gctacatcat tgcgcagcag gacaataaca gtcaggctat gcagtggctg
     2221 acccggttac gggataactc tcatcgcttc ggataacttc ctgttatccg aaacatatca
     2281 ctcactttgg tgatttcacc gtaactgtct atgattaatg aggcggtggt taacactacc
     2341 gcttcgttgt ctggacaatc gttcctttgt aataggtccg aatcacaatg gaaggttcaa
     2401 gaatgaaata ccgcatcgct ttagctgttt ctctctttgc tcttagtgcc ggtagttatg
     2461 ccactaccct gtgtcaggaa aaggagcaaa atatccttaa ggagatcagc tatgccgaaa
     2521 aacaccaaaa ccagaatcgt attgacggtc tgaataaagc cctgagtgaa gtccgggcca
     2581 actgttcaga tagccagctg cgtgccgatc atcacaagaa aatcgcaaag cagaaagatg
     2641 aggtggcgga acgccagcaa gatttagccg aggcgaagca aaaaggcgat gccgatagaa
     2701 cggaaactgg cagaagcgca ggaagagctg aaaaagctgg aagcgcgcga ctactaactc
     2761 acaatagtca ctacttactc acctggagaa aactatgtcg aaagaacaca ctacggaaca
     2821 tctgcgtgct gagttgaaat ccctttccga tacgctggaa gaggtgctta gctcatctgg
     2881 cgagaagtcg aaagaagagt tgagtaagat tcgtagcaaa gcggagcagg cactgaaaca
     2941 gagccgttat cgcctgggtg aaaccggtga tgccatcgcc aaacaaaccc gtgtcgcggc
     3001 ggcgcgtgct gatgagtatg tgcgtgaaaa tccgtggacc ggcgtgggaa ttggcgctgc
     3061 aatcggtgta gtgctcggcg ttctgctgtc gcgtcgttaa ttatggcgga cactcatcac
     3121 gcgcaagggc ccggtaaaag cgttctgggc atcgggcagc gaattgtttc catcatggtt
     3181 gaaatggtgg aaacacgtct gcggctggcg gtggtggagc tggaagagga aaaagctaat
     3241 ctctttcaac ttttactgat gctgggcctg acgatgcttt tcgctgcatt tggtcttatg
     3301 agcctgatgg tgctaattat ttgggcggtt gatccgcaat atcgcctgaa tgcgatgatt
     3361 gccaccaccg tggtgttgct gctactggca ctgattggcg gtatctggac gttacgtaaa
     3421 tcgcgtaaat ccaccttgct gcgccataca cgccatgagc tggcaaacga tcggcaactg
     3481 ctcgaggagg agtcccgtga gcagtaaagt cgaacgtgaa cgacgtaagg cgcaactgct
     3541 tagccagatc cagcaacaac ggctggatat ttccgccagt agtcgtgaat ggctggaggc
     3601 aacaggcgct tacgatcgtc gctggaatat gctgctaagt ctgcgctcct gggcgctggt
     3661 tggcagtaga gtgatggcga tctggacgat tcgccatcct aatatgctgg tccgctgggc
     3721 cagacgcggt tttggcgtat ggagcgcctg gcgtctggtt aaaacgaccc tcaagcagca
     3781 acagcttcgc ggttaacttc cctctggccg gagcaattcc ggccttatcc atcaaatttt
     3841 ttgaatattt ttgacagttt tccttgctaa caatcatcat tcaccacgtt tatgattctc
     3901 tccatcgaca gcaacgacgc taatagcgcg cccttgcaca aaaaaacaat cagcagcctg
     3961 agtggcgcag tggagagtat gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcac
     4021 attttaatgc cgattctgtt tattaccgca ggctggggaa aaatcaccgc ttactctggt
     4081 acccaacaat atatggaagc aatgggtgtc ccgggtttta tgctgctgac ggcattttta
     4141 tttcacagca actttgctga aggcgtcaac tcgctgatgt tcatgaaaaa cctgacaatt
     4201 tctggcggat tcctgctgct ggcaattacc ggtccgggcg cgtatagcat cggccgcctg
     4261 ctgaataaaa agtggtaagc acgcgctata cttaacgata aaaaacgagg aggaagctcc
     4321 tcgtttttgc tattggagga gagaaaaatg ggtcaactga ttgacggcgt ctggcatgac
     4381 acctggtacg ataccaaatc taccggcggt aaatttcaac gttcagcttc cgcatttcgt
     4441 aactggctca ctgccgatgg cgcacctggc cccactggca caggcggttt tatcgcagag
     4501 aaagatcgtt atcatctcta tgtttcactc gcctgcccgt gggcgcaccg cacgctgatc
     4561 atgcgcaaac tcaaaggact ggaaccgttt atttccgttt ccgtagtgaa cccgctgatg
     4621 ctggaaaacg gctggacctt tgatgacagt tttccgggag caaccggaga tacgctctat
     4681 caacatgaat ttctgtatca gctttatctc cacgccgatc cacactacag cggacgagtt
     4741 actgttcccg tgctgtggga caaaaagaac cacaccatcg tcagcaacga atcagcagaa
     4801 atcatccgca tgttcaatac cgcgtttgat gcgctgggcg caaaagcggg tgattactac
     4861 ccaccagccc tgcaaacgaa aattgacgaa cttaacggct ggatttatga caccgttaac
     4921 aacggcgtgt ataaagctgg ttttgccacc agccagcaag cctacgacga ggcggtggcg
     4981 aaagtgtttg aatcgctggc gcgactggag cagattttag gccagcatcg ttacctgacc
     5041 ggcaaccagc taaccgaagc cgatattcgc ctgtggacta cgctggtgcg ttttgatcca
     5101 gtgtatgtga cccacttcaa gtgtgataag caccgcatca gcgattacct gaatctgtat
     5161 ggcttcctgc gcgatatcta ccagatgccg gggatcgccg aaactgtcaa tttcgatcac
     5221 atccgtaatc attacttccg cagccataag accatcaacc ctacggggat tatttcaatt
     5281 ggtccgtggc aggatctcga tgaaccgcat ggacgagatg ttcgcttcgg ttaaaaaaag
     5341 gcaccttacg aggtgcctct gataattagc taagtgcatg ttgttcatgt cggatgcggc
     5401 gtgaacgcct tatcaaacct acaaagcgtt tatattcaat atgttacaat gaccgcgtag
     5461 gccagataag cgtagcgcat ctggcgattt tgcgcttgcc atcagtctta gcgcgccttc
     5521 acggcacttt attaattcct tgaacgaata tttactgcca tttcattcac gtctattctt
     5581 aatttgctgc tttaaaaaca agtgattgag caaaattgag gcaaaaatgg actggtatct
     5641 gaaa
//
LOCUS       DAJTSI010000198         5599 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000198 DAJTSI010000000
VERSION     DAJTSI010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.199"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..410
                     /locus_tag="OO327_003717"
     CDS             <1..410
                     /locus_tag="OO327_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6020088.1"
                     /translation="VRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDAL
                     CYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGID
                     PEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            442..1056
                     /gene="istB"
                     /locus_tag="OO327_003718"
                     /pseudo
     CDS             442..1056
                     /gene="istB"
                     /locus_tag="OO327_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="OO327_003719"
     CDS             complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="OO327_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HCR6020089.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1378 a         1402 c         1331 g         1488 t
ORIGIN      
        1 ccgtcaggca gcagcgaagc aaagcgttac tgacgtcgct gaatgaatgg atggtggaga
       61 agaatggtac gctgtcgaaa aaatccagac tgggcgaagc gttcagctat gtactgaatc
      121 agtgggatgc cctctgttat tacagtgatg acggtctggc ggaggcggac aataacacag
      181 cagaaagagc gcttcgtacc gtctgtctcg ggaaaaagaa ttacatgttc ttcggcagcg
      241 atcatggagg cgatcgcgga gctctgctgt acggactaat cgggagctgt cggttgaacg
      301 gtatcgatcc agaagcgtat ctgcgccata tcctgagcgt actgccggaa tggccttcca
      361 accgagttga cgaactgctg ccatggaacg tagttctcac cgataaataa gcgtcaatac
      421 ggccttccgt tgacgcttac agaactcctg tggcgcgaga gtgaaaaact gcgtcgttat
      481 aaaaaggagg cccggttgcc agttgccaaa acgctaagcg aatacgactt cattcagttg
      541 ccggaactga atggagctca gttccagcaa ctctgtgaaa cgacagactg ggttgatgct
      601 ggagaaaacg ttctgctgtt cggggccagc gggttgggga aaagccatct ggcggcagcg
      661 atcgtggatg gcgtagtagg ccagggctac cgggcccggt tctacagcgc aggagagctg
      721 ttgcaggaac tacgtaaagc cagagctcag ttgaaactga atgagctgct actgaaactg
      781 gatcgctacc gggtgatagt ggtggatgat cttggctatg tcaaacgcga cagcgccgaa
      841 acgggtgtgc tgttcgagtt aatagcgcat cgctatgaac gtgggagcct ggtgataacc
      901 agtaaccatc cgttcagcat gtggggcagc atcttcgtgg atgagactat ggcggtggcg
      961 gcagcagacc gactgatcca tcacggatat atgttcgaac tgaaagggga aagctacagg
     1021 aaaaagacag cgaaggcagt aacaagcgtg acttgatgtc gcactgaagg ggcggccagt
     1081 atagttggcg ccagtcggca aaactagttg acgtctaata cctggacggt cacttctgag
     1141 aaaaggcatt tacacagaat cttaaacagg ctcacggagt tctttcagaa aaaggcctgc
     1201 cgaatggcag acctatccca ttcgtcagaa aacataacgg aagttggcgt ttatcgcatt
     1261 atccacatta tatttcccga acgctgattt ctccagctcc agtcccaaac gcatattatc
     1321 cttaatttcc gcattcatcc ctaccgtcat cagcatcctg ctatcttttt caccttcgaa
     1381 acgtttctct ccggaagcat cctgcagaac cgtttctccg ttcgccagca ggtcgaactg
     1441 ataacccagc ccggctcgag ctgtgatttt ccagtcgtct ccggagaagg ctcttcccac
     1501 gtcaacacca gtacggccaa tcagtgggtt ataatccttg tctttcatgc tcagagccat
     1561 tccccggtct tcccagctaa aagcttttcc tgatacagaa ccgtaaacca gctctatctg
     1621 tggctccacc caggactctt tcgtcaggtg gtaacgataa ccaacttcag caccggcata
     1681 ccaggaatga gagctgtagt cttttgttcc cagtgaggca aaattcgccg tgtgctgatt
     1741 atcatgatgg agatatttac caatcaggtc aaaataagct ccggaattaa agagtgcaga
     1801 ggcatacaga ccgccaccca cggatttgtt ttttccactg aatgcgtggc tgcttgcatt
     1861 gctgtccgta taggtcagca atgcccccgt aaataaatcc acaccgtcca gctcatgctt
     1921 tctgtcgaca ccaatctgaa cgtgagtgta gttatcgctg tagtcaccat ctgccgaacc
     1981 ggtaccattc attatgcgtg cccagacacc ggcatccccc tgagtatccc gcaggtcacc
     2041 catacgtttg ttcaggttat tgacctctgt aaggaaggac ttataacctg atgccataaa
     2101 gtcttttgcg gctttcgagg ctccggcatc agcggtagtc tgatatcctg tcagcaccca
     2161 ttttgtggca tcatccgttt tttccgtgct gattactggc gtaacgttac tgaaaccaat
     2221 ctgctgcgta ccggcagtga acgttttctc atcactccct ttcggtgcgg taattaacgt
     2281 cacgctcatt tcactggctg gtttgtttaa aaagtcaacc aggacgctat tattactgcc
     2341 tgacagtttg tctgtgacat tcaactggtc agcccggctg ttgttggtat gcatcaggaa
     2401 cgtggtatta tctgcagaaa gctcttttac ttgtaaagtg gcaaagtgtt tcccgtcccc
     2461 cagttcaatt cgcgagccct tagccttcag tgcaccggcc tctgattttc cgtcagagta
     2521 ccacagagca ttgatcatat ttaccgtact ctgtgccccc gtaatactgc ctctcagaac
     2581 agcgttatat cccttcatta aggagctgaa taacggagag tccgtcttcc ctgcatcagc
     2641 atcactgtct cccagattaa tggttgccgc agtggttcct gcatgaatat ccgaactcag
     2701 atagcccata tttttaacag aaaagctggc gttatcttcg aggttaatcc cttctgttgt
     2761 agaaattacg ggtgagtaat acccctgttt ctgcgcagaa ggcatccccg tcagggtcag
     2821 agaagcattt tcttttacat ccacagcccc ggtgctgaaa ataccgctct gagccgtaag
     2881 ttttgctcct tttccgaggg tcaggctgct gttgacaaag ctgccaacac ggtcaaaaac
     2941 agcattctga gaggtcacac tcacggtgct atcataagcc tcaataccac cggtgaatct
     3001 ctccatgatt tgcagggatg atttattttc cagcgtaaca tttccgctgt aatcactctg
     3061 gtcaccaact gtggttgcag tggcactccc ttcaacagag gagaccgcat taccggttcc
     3121 gtcactcctg tcagtaaaga cacggctact tcccagaatg actgtacttc cattggtggc
     3181 gttaatatct ccctgcatct tgccgtttct ggacagatgg aatgttgccg catcgagttt
     3241 cagtttttcc agcgtaaact gacgggtttc ccagtcgctc tggctggaag aagtcgtcgt
     3301 tccagcatga ataactggat gaccctggaa tatcagcgtg ccggacttct tggttatcga
     3361 accttctggc aggttaactg aaccatcaag agccagtaca tccttacctg agagctgcgg
     3421 aatattcaca tccatattgc cgtttagttg gccgtgatac atataaggct ggctgctggc
     3481 ctcaattttt tgttgcttca cagtatcaat ggctttatta cgatcttttc cgacattctg
     3541 ccagacactg ctgttgttca gatcggagaa gaacggggaa tatgaactcg ctttcaggat
     3601 gaaatattgc ttggtagaac tgtcataata taggtctccc ggcgctcctc tccccccaaa
     3661 aatactgaca gtattaaccg gtacattgat gtcactggct tttaatgtct gaaggtcgag
     3721 tgttaccgtt gattttttct cactgttatt ggtaatgatg gccccgtagt cagccgcctg
     3781 aagacgggta aaggtcagat tattaccatt cagttccaga cgtcctccac ggtatcccca
     3841 cgaaatatta tccggattaa cttgctgtga gtcagaaagc acaactgtcg gacgaccgct
     3901 ggcaatacca acagagctga aagcctgtac ctttccgtca gcatccgcct gctggttgag
     3961 aataacggta ccgtccccca ccttgagtcc gcctgcattc acaccggtcc cgtttactgt
     4021 cagggtacct tcgccagttt tatgcaggtt atcaccggca actccgttca cctgccacag
     4081 aacgtgggtt cctttatcgg tgataatccc gccccccatc catgtctggc cgttcagggc
     4141 ggacaccctg taatcgccgg caaactgcag atacccagct ccctgattaa cggaattctg
     4201 cagaacgacc tcgccattat tgccggtgaa caggaggttt tttcctgcgt tcagatcatt
     4261 tcctttcttt ccatgcatat cccaagtcgt gtttccctga gtcagtgtgc cggtgccatt
     4321 agccgcatca tatttccact gcagtactcc ttcaccagag gtgtaggcta tagttttgtc
     4381 aaaatcattt tgcggttgct gaccaaggaa atcctgagtg gtgacgaccc agttatttcc
     4441 atagttagtt cctgaggaaa gcacgccaat cagtacccat tttttttgca gggaatcata
     4501 agcaaacagg ggggagccgc tgtcccccat attcagataa ttagccagag gtccattcgc
     4561 gggattaaaa atgtcaccgg tctgagctgt aatcatctgt ccgttgttat aggagttcag
     4621 cgcgctgacg gttcccccaa caagaaatcc gccattggta tacacttggg tgcgattacc
     4681 atttttgtct ttgacgtact gcatcccccc cccaaggcga tagaattcag taaagcgtcc
     4741 gccggcctga taggcaccgc tcacagcccc gatgtcagac acttctgccg gcgccacctc
     4801 tgtcaccagt ttactcaggc ggcgggtttt aatatcaaga ccgctattat tatttgtacc
     4861 aaccgcagta taggtgtttt tggcgtatcc gaagctcatc gtgtcagaac cgctgacatg
     4921 tttggcggta accacatact gaggggagta aagtgtatgg tctcccggag gcaggctacc
     4981 ggtagtaata gtggcactac tgaaatcagc catcggggct ttatcaagtt taccgaccag
     5041 attgccctgc ttgtcgtaaa tggaaatatt cgtggtcccc ggcgtaaact gacctttatt
     5101 ctcggcaaag tcgcggaata tctgataagg gatctctgca ctgaccgttg ctgacagagc
     5161 tggccatgcc gaggaaagtg ataaagctgc aacagacgta agaataaccc gacgtgaaag
     5221 tctgcgatgg cttttgcagg taaccctgcg ggccagttcg gagacagcaa tcaggctttt
     5281 ggttatatgg caatacttaa gataatatat tttgttcata tagaaacatc aacctaattt
     5341 tgacaaagat taattacata ggatgtagat ggagcagtcc gagattctac taatatcaat
     5401 tattaagcat ctgtttttta gttggtgtaa tataattttt attaaggtaa gcggaaaaac
     5461 aaccaaaaaa aacgcaagat gttatttttt tatcattttg agccagtttg gctgggggga
     5521 tactcttctc ttaagcttct tgctcaactt taaaaatagt tatgccagtt cggtatcgat
     5581 aaggggcagt ttggatatc
//
LOCUS       DAJTSI010000199         5590 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000199 DAJTSI010000000
VERSION     DAJTSI010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.200"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            19..876
                     /locus_tag="OO327_003720"
     CDS             19..876
                     /locus_tag="OO327_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCR6020090.1"
                     /translation="MAIGVLAVVVLPFLSYYTVNEGERGILLRYGKIVKVAEPGLGFK
                     IPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFHIKPSEAGAVYTTYNTIE
                     ALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNAMRKAVVGPVVIDGVQIE
                     NIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVTQAQAEADSKLAAAKAEA
                     ETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVALTTAERWDGKLPDTMIPG
                     STVPFISTK"
     gene            complement(922..2913)
                     /gene="tkt"
                     /locus_tag="OO327_003721"
     CDS             complement(922..2913)
                     /gene="tkt"
                     /locus_tag="OO327_003721"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6020091.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3191..3949
                     /gene="loiP"
                     /locus_tag="OO327_003722"
                     /gene_synonym="yggG"
     CDS             3191..3949
                     /gene="loiP"
                     /locus_tag="OO327_003722"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR6020092.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4155..5075)
                     /gene="speB"
                     /locus_tag="OO327_003723"
     CDS             complement(4155..5075)
                     /gene="speB"
                     /locus_tag="OO327_003723"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR6020093.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5211..>5590)
                     /locus_tag="OO327_003724"
     CDS             complement(5211..>5590)
                     /locus_tag="OO327_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020094.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVN
                     AGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1343 a         1493 c         1496 g         1258 t
ORIGIN      
        1 ccacaaaaat ctcttgctat cgccattggc gtgcttgccg tggttgtgct gccatttctc
       61 tcgtattaca ccgttaatga aggtgaacgc ggcattttgc tgcgctacgg aaaaatcgta
      121 aaagttgccg aaccggggct gggttttaag atccctttta tggaaagcgt ggagaaaatt
      181 tccacgcgta atcaggctgt ggtctatcag ggattacagg cctacagccg cgaccagcaa
      241 ccggcacaaa tgacagtatc agtcagcttc cacattaaac cttcagaagc aggcgcggtt
      301 tataccacct acaacactat tgaagcatta aaagatcgtc taattgtccg tcagttgcca
      361 actcagctgg agaacatttt cggccaatat accgccatca gcgccgtaca ggatcgtaca
      421 aaattggttc aggatctgca aaatgccatg cgcaaagccg ttgttgggcc agtggtaatt
      481 gacggggtcc agattgaaaa tattgatttt tctgacgctt atgaaaagtc gattgaaaac
      541 cgaatgaaag cagaagtcgc cattgccacg cgtaagcaaa atctggaaac agagaaaatc
      601 caggcgcaaa tagcggtaac tcaggcccag gcagaagcag acagtaaact ggcggcagcc
      661 aaagcagaag cggaaactat tcgcgtaaga ggcgcggcag aggctgaaac tattcgttta
      721 aaaagtgcgg ctgaagcaga agcaatccgc ctgcgcggtg aagctctgcg ggataatccc
      781 ggacttgtag ccctcaccac tgccgaacgt tgggacggta aactgcccga taccatgatc
      841 cccggcagca cagttccgtt tatctctact aagtaaaaaa agggccgctt tcgcggtcct
      901 tctcacaaaa cgtcttactg attacagcag cgcttttgct ttcgcaacaa cgttatcaac
      961 agtgaagccg aactcttcaa acagcagctc tgccggagca gattcaccga aggtggtcat
     1021 gccgacgata gcgccgttca ggccaacata cttgtaccag tagtcagcaa tacccgcttc
     1081 taccgcaacg cgtgcagtaa ccgctttcgg cagtacggat tcacggtaag ccgcatcctg
     1141 cttgtcaaat gcgtcggtag acggcatgga caccacgcgc gctttcacgc cttcggcagt
     1201 cagtttttcg taggcagcaa ccgccagttc aacttcagaa ccggtggcga tgaagatcag
     1261 ttccggctga ccagcacagt ctttcagcac ataaccaccg cgcgcgatgt ttgccagctg
     1321 ctcttcagta cgttcctgct gcgccaggtt ctgacgggag aggataagcg cagtcgggcc
     1381 gtcctgacgc tcaacgccgt atttccacgc gaccgcggat tcaacctggt cacacggacg
     1441 ccatgtagac atgttcgggg tcacgcgcag agaagcgacc tgctctaccg gctggtgagt
     1501 cgggccatct tcgcccagac cgatggagtc gtgggtgtaa accatcacct ggcgctgttt
     1561 catcagcgca gccatacgta cggcgttacg tgcgtattcc acgaacatca ggaaggtgga
     1621 ggtgtacggc aggaagccac cgtgcaggga gataccgtta gcaatcgcgg tcataccgaa
     1681 ctcgcgaaca ccgtagtgga tgtagttacc tgcagcatct tcgttgattg ctttagaacc
     1741 agaccacagg gtcaggttag acggtgccag gtcagcagag ccgccgagga attcaggcaa
     1801 cagcgggccg aacgcttcga tagcattctg cgacgcttta cggctggcga ttttcgccgg
     1861 attagcctgc agtttagcga taaactcttt cgctttggcg tcgaagtcag acggcatttc
     1921 gcctttcata cggcgggtaa attcagccgc ttcctgcgga taagctttcg cgtaagccgc
     1981 aaacttctca ttccatgcag attctttcgc ctggcctgct tctttcgcat cccactgagc
     2041 atagatttca gacgggattt cgaacggtgc atatttccag cccagctgtt cgcgggtcag
     2101 ggcaatttca gcgtcgccca gcggcgcacc gtgggagtcg tgagtaccgg ctttgttcgg
     2161 ggaaccgaaa ccgatgatgg ttttgcacat cagcagggac ggtttgtcag tcactgcgcg
     2221 cgcttcttca actgcgcgtt tgatggatgc cgcgtcatga ccgtcgatgt cgcgaataac
     2281 gtgccagccg taagcttcga aacgcattgc tgtgtcgtcg gtgaaccagc cttcaacgtg
     2341 accatcgata gaaataccgt tgtcatcgta gaacgcaatc agtttaccca gcttcagcgt
     2401 acccgccaga gagcaaactt cgtgggagat gccttccatc atgcagccgt cgcccatgaa
     2461 ggcgtaggtg tagtggtcga caatgtcgtg gcccggacgg ttaaactgcg ccgccagcgt
     2521 tttttctgca atcgccatac cgactgcgtt ggcaataccc tgacccagcg gaccggtggt
     2581 ggtttccaca ccagcggtat aacctacttc cggatggccc ggagttttag agtgcagctg
     2641 acggaagttt ttcagttctt ccatcggcag atcgtaaccg gtgaggtgca gcaggctgta
     2701 gatcagcatg gagccgtggc cgttggacag cacgaagcgg tcacggtcag cccaggacgg
     2761 attctgcggg ttgtgtttca ggaaatcacg ccacaggact tcggcaatgt cagccatacc
     2821 cataggggca cccgggtgac cggatttggc tttctgtact gcgtccatgc tcagcgcacg
     2881 aatagcattg gcaagctctt tacgtgagga cattttgact ccagatcgga tgatgaaggg
     2941 cacgccctta acgacttgac gacagcgcgt tttgggctac gccggaaaat ttgccaacaa
     3001 tttaccgcaa gccgcgcgtc atgtacatgg aacatccttt tgccgcttca gaaatctctg
     3061 gatcatgctc gcatgttgcg caatctgctc gcccgtccgc tgcgcttttc cttatactga
     3121 gactgagcgt cgattcacct gcaaacggcg catttttaga ataatcctga ccttgtgcgg
     3181 aagagaaaac atgaaaattc gcgccttatt ggtagcaatg agcgtggcaa cggtactgac
     3241 cggttgccag aatatggact ccaacggact gctctcatca ggagcggaag cttttcaggc
     3301 ttacagtttg agtgatgcgc aggtgaaaac cctgagcgat caggcatgtc aggagatgga
     3361 cagcaaggcg acgattgcgc cagccaatag cgaatacgct aaacgtctga caactattgc
     3421 caatgcgcta ggcaacaata tcaacggtca gccggtgaat tacaaagtgt atatggcgaa
     3481 ggatgtgaac gcctttgcaa tggctaacgg ctgtatccgc gtctatagcg ggctgatgga
     3541 tatgatgaca gataacgaag tcgaagcggt gatcggtcac gagatggggc acgtggcgtt
     3601 aggccatgtg aagaaaggga tgcaggtggc gcttggtaca aatgccgtgc gagtagctgc
     3661 ggcctctgcg ggcgggattg tcggcagttt atctcaatca caacttggtg atctgggcga
     3721 gaaattagtc aattcgcaat tctcccagcg ccaggaagca gaagccgatg attattctta
     3781 cgatcttctg caccaacgcg gcatcagccc ggcaggtctt gccaccagct ttgaaaaact
     3841 ggcgaagctg gaagaaggtc gtcaaagctc aatgtttgac gaccatcctg catccgccga
     3901 acgtgcccag catattcgcg atcgcatcag cgcggatggg attaagtaaa gcctagtggt
     3961 gtcgaacgta ggtcagataa ggcgttcacg ccgcatctga cattaatggc acatttgtta
     4021 ccttgtgcgc attgccggat gcgatgctgg cgcatcttat ccggcctacg ggtgccgaac
     4081 gtaggtcgga taaggcgttc acgccgcatc cgacattaat ggcacgtttt acccgtgcgc
     4141 atcgcatctg gtgcttactc gccctttttc gccgcctgaa tatacagcat ctccagcgcc
     4201 agcgtcgccg cagccagagc ggtgatttcc gactgatcgt atgccggagc cacttccact
     4261 acgtccatcc caacgatgtt gagatctttc aggccgcgta ccagtttaat ggcgcgatcg
     4321 gaggtcaggc cgccaatcac tggcgtaccg gttcccggcg cgaaagcagg atccaggcag
     4381 tcgatatcaa aagtcaggta aaccggcata tcacccacaa tctgtttcac ctgggcgata
     4441 acgtcatcca cgctgcgatc gttcacctgg caggcgtcca gcacggtaaa gccgttgtct
     4501 ttatcaaact cggtacgaat accaatctgc acggaatgat tcgggtcgat cagaccttct
     4561 ttcggcgcgg tatagaacat agtgccgtgg tcaaattcac aaccgttcgc ataggtatcg
     4621 gtgtgggcgt caaagtgtac cagcgccatt ttgccgaaat gcttcgcatg agcacgcagc
     4681 agcggcagcg taacaaagtg gtcaccaccg aaggagagca tacgcttacc ggcagccagc
     4741 agcttctcgg cgtgcgcctg cagcttttcg ctcatctcac gggcatcgcc aaaggcatat
     4801 accagatcgc cgcagtccac gacgttcaga cgctcacgca tgtcgaaatt ccacgggaag
     4861 cggttgtgtt cccaggccag attcgtcgaa acctgacgga tcgctgccgg accgtggcga
     4921 ccacccgcac gaccagaagt ggccatatcg aacggcacgc cagtaatcac ccagtctgca
     4981 tcgctgtcat acggctggaa gttcatcggc aggcgtaaaa aaccaaaggc attggaaacc
     5041 agtgagttat cgtattgatg acctaaggtg ctcatggatg gactcctatt acaaagtcga
     5101 tataaaaaaa cccttccgcg tcgttaggcc cgacgaggaa gggttggatt tgcgaaacac
     5161 aagtttgtgc taattatcgc cttaaatggg cgaggcatca agcgaggttg ctatttccga
     5221 cacggtggcg gcgcatagcc attgttatct gccggaaagg cttgtccaga gcacggcggc
     5281 agcgcgtttt gcgccgggcc ttgtaattca aaggcggcaa agccgattac cccggtatta
     5341 cgcacatcgc cttgcgccga attggctgcg taggaatcag caacgttggc gaactgaaaa
     5401 gccgcttcag tatgtttatc tttgcggaat cctttaatca ctaacgaatc tccggcattc
     5461 acaatatagc cgttgttatt cagcgaacct tgcttgccgt tcagcacatc caggccatca
     5521 gccgtagcaa cgatctcgta gttggtgtta cgactgtaat tacggacata taattgataa
     5581 ttcattccga
//
LOCUS       DAJTSI010000200         5557 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000200 DAJTSI010000000
VERSION     DAJTSI010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.201"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..641
                     /locus_tag="OO327_003725"
     CDS             <1..641
                     /locus_tag="OO327_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HCR6020095.1"
                     /translation="VGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAITT
                     GKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNA
                     VLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCAL
                     GLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(695..1300)
                     /gene="azoR"
                     /locus_tag="OO327_003726"
     CDS             complement(695..1300)
                     /gene="azoR"
                     /locus_tag="OO327_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR6020096.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1501..5403
                     /gene="hrpA"
                     /locus_tag="OO327_003727"
     CDS             1501..5403
                     /gene="hrpA"
                     /locus_tag="OO327_003727"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR6020097.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQN
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLRYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1378 a         1359 c         1539 g         1281 t
ORIGIN      
        1 tcgtaggcgc gtgcattgtg ttgatggagc taatgatgat ggttcagtta tggtggtcag
       61 tctggttatg ttggccagta ttatcgctac tcattattgg ccgtgggtgc ggtgggcttg
      121 gcgcgataac aacagggaaa gatagtcagg ggaaactccc gcccgccgtt tactggctga
      181 cattgccctg gcgcatcggg atgtggctat ctatgcgttg gttttgtcgt cgcctggagc
      241 cggtgagcaa aatgactgct ggtgtttatt taagggcgtt tccacgacat attccggcac
      301 agaatgcggt tctggacgtc acctttgaat tccctcgtgg acgagcgaca aaagatcgac
      361 tctatttctg tgtaccgatg ttggatctgg tggttccgga agagggggag ctccgacagg
      421 ccgtggcgat gctggaaaca ttacgcgaag agcaaggcag cgttctggtc cattgcgcat
      481 tgggattatc gcgcagtgcg ctggtagtgg cggcatggct gttatgttac ggacactgta
      541 aaacagttga tgaagcgatt atctttattc gagccagacg ctcgcatatt gtgcttaagg
      601 aagagcacaa agcgatgttg aaattatggg aaaacaggta agtggattga gatgtggact
      661 gaatatctac agtccacatc aagaccgtgt ccggttatgc agcaacaatg ctgtcgatgg
      721 ctgcttttgc gtcagactgt gctttcgctg ccatttccgg accgtatgcg atcccttcgg
      781 cgaagacaaa tttcacatcg gtaatgccga taaagccgag gaacgtggac agatacggcg
      841 tcaccaggtc cgttggtcca tctttgtgga tcccaccgcg gctggtaata acgatggctt
      901 ttttacccgt taccagacct tccggaccgt tctcggtata gcggaaagta acgcctgcgc
      961 gtgcaaccag gtcaaaatag tttttcaact gagttgagat gttgaagtta tacatcggtg
     1021 ccgcaataac gataacgtcg tgggctttca gctcggcaat cagctcatcg gaaagcgcca
     1081 gagcttcctg ctgacgcgga gtcagcggcg catcgctcgg acgcagagcg ccaaccagtt
     1141 cgccatccag taccggaatc ggatttgcag ctaggtcgcg aacggtgatt tcatcagcgg
     1201 agtgcttttc gctccattgt tcaacaaaat aatcggacaa ctgattagac tgagagtacc
     1261 ctgccaggat gctggattta agaactaata ccttgctcat ggtgtttcct tatagatgtt
     1321 tgaatggacg atgccccgtt gcttgctgac actttattca caatcctgcc acagagatcg
     1381 cgcaataaat cgaagtctat gttcgaattt attgaacaac gcatagaaag ccgcgatgtg
     1441 gtactctata tctatcattt aaaagaaaat taatcaggca gactactgcc cactaacgtt
     1501 atgacagaac aacaaaaatt gacctttact gccttgcaac agcggctgga ttcgctgatg
     1561 ctgcgcgaca gactgcgttt ttctcgccgt ctgcattgtg ttaagaaggt taaaaatcct
     1621 gatgcacaac aggccatttt ccaggagatg gcgaaagaga ttgaccaggc ggcagggaaa
     1681 gtcctgctgc gtgaagcggc acgaccggaa atcacttatc ctgacaattt accggttagt
     1741 cagaaaaaac aggacatact cgaagcgatt cgtgatcacc aggtggtgat cgtcgccggg
     1801 gaaaccggct ccggtaaaac gactcagcta ccgaaaatct gtatggagct ggggcgcggt
     1861 attaaagggc tgatcggtca tacccagccg cgtcgcctgg cggcacgaac ggtggccaac
     1921 cgtattgcgg aagagctgaa aacggagccg ggcggttgca tcggttacaa agtgcgtttc
     1981 agcgatcacg taagtgataa cacgatggtc aagctgatga ccgacgggat cctgctggcg
     2041 gagatccagc aagaccgcct gctgatgcag tacgacacca tcattattga cgaagcgcac
     2101 gaacgcagcc tgaatatcga ttttctgctc ggctatttga aagagttgct gccgcggcgt
     2161 cctgacctaa aaatcattat cacttccgcg actatcgacc cggaacgctt ttcgcgccac
     2221 tttaataatg cgccgattat tgaagtctcc ggtcggacct atccggtgga agtgcgctat
     2281 cgcccgattg ttgaagaagc cgatgacacc gagcgcgatc agttgcaggc gatttttgac
     2341 gccgtagacg aactaagcca ggaaagcccc ggcgacattc tgatctttat gagcggcgag
     2401 cgggaaattc gcgataccgc cgatgcgctg aacaagctga acttacgcca taccgaaatc
     2461 ttgccgcttt atgcgcggct ttcgaacagc gaacagaacc gcgtgttcca gtcgcacagc
     2521 ggacggcgca ttgtgctggc gaccaacgtc gcggaaacct cgctgaccgt accggggatt
     2581 aaatacgtca tcgaccccgg tacagcgcgt atcagccgct acagctatcg caccaaagtg
     2641 cagcgtttgc cgattgaacc catttcgcag gcgtctgcca atcagcgtaa aggccgctgt
     2701 ggtcgtgtgt ccgaagggat ctgtattcgt ctctattccg aagacgattt cctctcgcgc
     2761 ccggagttta ccgatccgga gattctgcgt accaacctgg cctcggttat tttgcagatg
     2821 accgcgctgg ggctgggcga tatcgctgcg ttcccgtttg tcgaagcacc ggataaacgc
     2881 aatatccagg atggcgtgcg tctgctcgaa gaactgggcg cgatcaccac ggatgaacag
     2941 gccagcgcct ataaactgac gccgctcggt cgccagctct cgcagttgcc tgtcgaccca
     3001 cgtctggcgc gtatggtgct ggaagcgcaa aaacatggct gcgtgcgtga ggcgatgatt
     3061 atcacgtccg cgctctccat ccaggatccg cgcgaacgtc cgatggacaa acagcaggca
     3121 tcggacgaaa aacatcgtcg cttccacgac aaagaatccg acttcctcgc gtttgtaaat
     3181 ctgtggaatt atcttggcga gcagcaaaag gcgctttctt ccaacgcctt ccgtcgcctg
     3241 tgtcgtaccg attacctcaa ctatctgcgc gtgcgcgaat ggcaggatat ctacacccag
     3301 ttgcgccagg tggtgaaaga acttggcatt ccggtcaaca gcgaaccggc ggagtatcgt
     3361 gaaattcaca tcgccttact gaccggtttg ctttcccata tcggcatgaa agatgccgat
     3421 aaacaggaat ataccggtgc acgtaacgcg cgtttctcca tcttccccgg ttctggttta
     3481 ttcaaaaagc cgccgaaatg ggtaatggtg gcggaactgg tagaaaccag ccgcctgtgg
     3541 gggcgcattg ctgcgcgtat cgacccggaa tgggtagaac ccgttgctca gcatttgatt
     3601 aaacgcacct acagcgaacc gcactgggaa cgggcgcagg gcgcggtgat ggcaacggaa
     3661 aaagtcactg tttatggttt gccgattgtt gctgcgcgca aggtcaacta cagccagaac
     3721 gattcggcgt tatgtcgtga actctttatt cgccacgctc tggtggaagg tgactggcag
     3781 acgcgtcacg cattcttccg tgaaaacctg aaactgcggg ccgaagtgga agagctggaa
     3841 cacaaatcac gtcgccgcga tattctggtt gatgacgaaa cgctgtttga gttctacgac
     3901 cagcgcatca gccacgatgt aatctccgct cgtcacttcg atagctggtg gaaaaaagtc
     3961 agccgcgaaa cgcctgattt gctcaacttt gaaaagagca tgttgatcaa agaaggggcg
     4021 gaaaaaatca gcaagctgga ttacccgaac ttctggcatc agggcaatct caagctgcgt
     4081 ttgagatatc agtttgagcc aggcgcggac gcggacggtg tgaccgtaca tattccgctg
     4141 ccgttactta accaggttga ggaaagcggg tttgaatggc agatccccgg tctgcgccgc
     4201 gaattggtga ttgctctgat taaatcgttg ccgaaaccgg tacgccgtaa ttttgtgcct
     4261 gcaccaaact acgccgaagc gtttttaggc cgcgtcacac cgctggagtt accgttgctc
     4321 gacagccttg agcgcgagtt acggcggatg accggcgtta ccgttgaccg cgaagactgg
     4381 cactgggatc aggtgcccga tcacctgaaa atcaccttcc gcgtggtgga tgacaaaaac
     4441 aagaagctaa aagaagggcg ctcgctacaa gatctgaaag atgcgctgaa aggcaaagtg
     4501 caggaaacgc tgtccgcggt ggcggatgac ggtatcgagc agagcggctt acatatctgg
     4561 agttttggtc agctgccgga aagttacgaa cagaagcgtg gcaactataa agtgaaggcg
     4621 tggccggcgc tggtggatga acgcgacagc gtgacgatca aactgtttga taacccgctg
     4681 gagcaaaaac aggcaatgtg gaatggtctt cgccgtctac tgctgctaaa tatttcctcg
     4741 ccgatcaagt atttacatga gaagttacca aacaaagcca agctggggct gtactttaac
     4801 ccgtatggca aagtgctgga gctgatcgac gactgtatct cctgcggtgt ggatcaactg
     4861 atcgacgcca atggtggccc ggtctggacg gaagaaggct ttgctgcgct gcatgaaaaa
     4921 gtgcgcgccg aactgaatga cacggtggtg gatattgcga agcaggtcga gcaaatcctc
     4981 actgcggtgt tcaatatcaa caaacgtctg aaagggcggg tggatatgac catggcactg
     5041 gggctttctg atattaaagc gcagatgggc gggttggtat atcgcggttt tgtcactggt
     5101 aacggcttca aacggctggg cgacacgctg cgatatttgc aggcgattga aaaacgactg
     5161 gaaaaactgg cggttgatcc acatcgcgat cgtgcacaga tgctgaaagt cgaaaacgtc
     5221 cagcaggcgt ggcagcaatg gatcaacaaa ctgccgcctg cacgtcgtga ggatgaagac
     5281 gtgaaagaga tccgttggat gatagaagag ctgcgcgtta gttacttcgc tcaacaactt
     5341 ggtacgcctt atccgatttc agataagcgt attttgcagg cgatggagca gattagcggt
     5401 taaccctgct atttgcctaa taaatctaaa acccggtaag catttagcgc cgggtttttt
     5461 attaattcta aaacggtaag gacaaaaatt caggaattca gaaaaataca attctcagct
     5521 gcaagatgaa taatgtttat ctacagcatt tccttaa
//
LOCUS       DAJTSI010000201         5554 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000201 DAJTSI010000000
VERSION     DAJTSI010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5554
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.202"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..147
                     /locus_tag="OO327_003728"
     CDS             <1..147
                     /locus_tag="OO327_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706949.1"
                     /note="chaperone-like protein that participates in the
                     polymerization of curlin (CsgA) subunits into curli
                     (extracellular fibers from Escherichia and Salmonella spp.
                     that are involved in the colonization of inert surfaces
                     and biofilm formation); part of the curli secretion and
                     assembly protein complex; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgE"
                     /protein_id="HCR6020098.1"
                     /translation="IFQTFLFPLKRDSEKTVVFALIQTEEALNRRQINQALLSTDDLA
                     HDEF"
     gene            172..588
                     /gene="csgF"
                     /locus_tag="OO327_003729"
     CDS             172..588
                     /gene="csgF"
                     /locus_tag="OO327_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCR6020099.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            615..1321
                     /locus_tag="OO327_003730"
                     /pseudo
     CDS             615..1321
                     /locus_tag="OO327_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(1454..1945)
                     /locus_tag="OO327_003731"
     CDS             complement(1454..1945)
                     /locus_tag="OO327_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR6020100.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(2047..2601)
                     /gene="ycdY"
                     /locus_tag="OO327_003732"
     CDS             complement(2047..2601)
                     /gene="ycdY"
                     /locus_tag="OO327_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR6020101.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSEREMLL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(2625..3362)
                     /gene="ycdX"
                     /locus_tag="OO327_003733"
     CDS             complement(2625..3362)
                     /gene="ycdX"
                     /locus_tag="OO327_003733"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR6020102.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(3417..4355)
                     /gene="ghrA"
                     /locus_tag="OO327_003734"
                     /pseudo
     CDS             complement(3417..4355)
                     /gene="ghrA"
                     /locus_tag="OO327_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            4590..4677
                     /locus_tag="OO327_003735"
     tRNA            4590..4677
                     /locus_tag="OO327_003735"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4624..4626,aa:Ser,seq:gga)
     gene            complement(4827..5108)
                     /locus_tag="OO327_003736"
                     /pseudo
     CDS             complement(4827..5108)
                     /locus_tag="OO327_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(5390..>5554)
                     /locus_tag="OO327_003737"
     CDS             complement(5390..>5554)
                     /locus_tag="OO327_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6020103.1"
                     /translation="RSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWA
                     ITTQKKEYQA"
BASE COUNT         1464 a         1393 c         1268 g         1429 t
ORIGIN      
        1 attttccaga cttttttatt cccgttgaaa agagactccg agaaaactgt cgtctttgca
       61 ctgattcaaa ctgaagaagc actaaatcgt cgccagataa atcaggcgtt attaagtacg
      121 gacgatttgg cgcatgatga attctaaata aaaaattgtt cggaggctgc aatgcgtgtc
      181 aaacatgcag tagttctact catgcttatt tcgccattaa gttgggctgg aaccatgact
      241 ttccagttcc gtaatccaaa ctttggtggt aacccaaata atggcgcttt tttattaaat
      301 agcgctcagg cccaaaactc ttataaagat ccgagctata acgatgactt tggtattgaa
      361 acaccctcag cgttagataa ctttactcag gccatccagt cacaaatttt aggtgggcta
      421 ctgtcgaata ttaataccgg taaaccgggc cgcatggtga ccaacgatta tattgttgat
      481 attgctaacc gcgatggtca attgcagttg aacgtgacag atcgtaaaac cggacaaacc
      541 tcgaccatcc aggtttcggg tttacaaaat aactcaaccg atttttaagc cccagcttca
      601 taaggaaaat aatcatgcag cgcttatttc ttttggttgc cgtcatgtta ctgagcggat
      661 gcttaaccgc cccgcctaaa gaagccgcca gaccgacatt aatgcctcgt gctcagagct
      721 acaaagattt gacccatctg ccagcgccga cgggtaagat ctttgtttcg gtatacaaca
      781 ttcaggacga aaccgggcaa tttaaaccct acccggcaag taacttctcc actgctgttc
      841 cgcaaagcgc cagggcttac aaaacctgct taacgagcgc aaggttattc gtgcggcaca
      901 agaaaacggc acggttgcca ttaataaccg aatcccgctg caatctttaa cggcggcaaa
      961 tattatggtt gaaggttcga ttatcggtta tgaaagcaac gttaaatcgg gcggtgttgg
     1021 ggcaagatat tttgggatcg gtgcagacac gcaataccag ctcgatcaga ttgccgtgaa
     1081 cctgcgtgtc gtcaatgtga gtaccggtga gatcctctcc tcggtgaaca ccagtaagac
     1141 gatactttcc tatgaagttc aggccggggt tttccgcttt attgactacc agcgcttgct
     1201 ggaaggggaa gtgggttaca cctcaaacga acccgttatg ctgtgtctga tgtcggctat
     1261 cgaaacaggg gtcattttcc tgattaatga tggtatcgac cgtggtctgt gggatttgta
     1321 aaataaagca gaacggcaga atgacattct ggtgaaatac cgccatatgt cggttccacc
     1381 ggaatcctga cagataaaaa tgccggaatt aataatccgg catttactgt ttaagcgaaa
     1441 gcgtgagtgc ccctcacgct ttatttttga tttcctgctc acggtgagcc gtctttgcac
     1501 tacgcgccgc cagccacagg ccgctgtttt tcattgcgta accaaatacc agcccaagcg
     1561 ccagcgaagg taacaccagt ttccagtcac cctgcccggc aaacgtcgcg catgcgccta
     1621 taaacgtccc cggaacaaat gaaagcagca gctgtttggc ctgaatacac atcagaaacg
     1681 cgacaatacc ggttatgaca taaccgagaa tttccagatg tggtgccagt gcactaccgt
     1741 aaataatgac catcgcccac accacgccac taagcagggt tgcagcggag atcgccagcc
     1801 ctttcagccc accttgcggg caagcaaagt aggccgtaca gcccaggaag cccgcccagc
     1861 ttagtaagcc cagggaaaca gccacccatc cccagatacc ggagagaatg cctgttgtga
     1921 ttgcaataga gagaagtatg ttcatgccgc gcatgatagc agaaaacgcg gccatgaatg
     1981 agatcgagca cacattttat gcaacaacaa attatctgtt gcagttatga gtgacgatga
     2041 tcacatttac tcttcagaat cttcttccag ctcgtcccac attgcactaa tggcatcacg
     2101 ggttagcggt gccatggtgc gccagaaagg cgtggttgca tgggcctcca ctttgccgag
     2161 gaacgcacca caccagggta acagatactc actgaacagt gtttccagtg cttcgctctc
     2221 atcttccgtt gactgatctt ccagccagga agccgcaagc agcaatgtgc cgatgtgatc
     2281 ggctggcgta tccgccagta gcatctctcg ttcggaaaga aaagcgcgca cttccgcttc
     2341 cgttgcgtcc tcaacccatg cgctacgata tggcggcacg gcacattcat cgccgataaa
     2401 caacgcattg taatcggcag agacttgcgt catatcacag cttttctgta aacgcgtcag
     2461 taactcatcc tgctccagtg gccagttcgc agccagtttc ccctcacgaa tcagagtaaa
     2521 cagcggcacc agtaaaggat cttgcggttg gcggtaatag agcgaaccca gcacacgaca
     2581 gaggatagaa aactcgttca ttaatatatt tccattacta gtaattaaag gtctgcaaat
     2641 tccgcaatcg gtgccatacc gcgagattca aggaagttca gtaagcggcg cggagaaaca
     2701 ttcaaaatgc gctctagcgg aaaatctacc gcgtcgagga ttttaagaca ctcttcaaat
     2761 tcccccatgg taaacgcggt gtgagaatcc gagcctaatg ccacccaacc tccagcatca
     2821 cgtaccgctg cagctaccgc acgacagttg tcttcactgc ccttacgtga gtgtaaaaat
     2881 gaggaattat tgatttccag cgccacctga tgtttcgcgg ctgcttcagc aacggcttta
     2941 acatctattt catatttcgg atttccggga tggcttatta tatgcacatt gccgctggcg
     3001 atagtggaga tcatcgcttg tgtattggtc gctttgtcat gtggcgcaaa aaccggctca
     3061 tgaaaaccgg caataatcag atccagcgag tcaaacattt taccgctgca gtcaatttca
     3121 ccatcaacat ttttaatgtt agcttcaatg ccgcgcagga tccctacccc atcaaccact
     3181 cgcggccaga tacgcatgtt aatgaagtgc cagtgatgcg gcgcatcttc catatccggg
     3241 ccatgatcgg tgatcgcaaa aagtttaatg cccttttgtt tggcctgggc aatgtaatca
     3301 ctcaatgtgc tatatgcatg tgtgctggca acggtatgca tatgaaggtc gacgggatac
     3361 ataactctct cctgtattca attcccgcca aggatagcag gaatcctgat gctttattag
     3421 tagccacgtg cgcggtcgac ttgcccgcag accctctccc ctttttcgag ctgggcaata
     3481 gtgcgagaaa tgtactccac agcttcagcg ggacgggtaa tcgcggcgac atgtggtgtt
     3541 atcgtcacgc gtggatgttg ccagagcgga ctttcaggcg gtaagggttc acgattaaaa
     3601 acatccagca ttgcgccttt aactttcccg ctatccagcg ccgcgagcag gtcatcttcc
     3661 acaacatgaa caccacgcgc caggttgagg agatacgcgc catcaggtaa tttttcgagt
     3721 aattgttgat taataatgcc gacggtttca ggggtattcg gtaacaaatt aatcaatacc
     3781 cgacattggc tcagaaatgc agacagttct tcccgtccgg caaagctttg cacgccaggc
     3841 cacgatttac gggttcgact ccagcaacgc agcggaaagc gctaggtttg cagactctga
     3901 gcaactttac agcccagtac gcctgcgccc aaaatgccga tggtaaaatc ttcccgatga
     3961 tattcaggca gcggttgcca atgcgaacta ttttgctgga tgcgataatc gtcaaaacgt
     4021 cgaaaccaat gcagcacctg actgacagca tattcctgca tttgctcgcc cataccggta
     4081 tcttccaggc gaaaaagtgg aacagaaggg ttcagcattt cagggtgtgc ctgtagcttg
     4141 ctcaaaatag aatcaacacc ggccccgagt gcgaacaccg ctttaagatc gcgccctgcc
     4201 agcatttcaa caggaggatg ccagactaaa acataatcag cagagtcatt atctccgctt
     4261 ttccatgccc tgactcttgc ctgaggaata gctttgcgca gtgcctcaat ccaccattgg
     4321 gtatcgaacg ttgggtgata aaagatgata tccatactga ctcccgaaaa gcgttgtgcg
     4381 aaatttattc gcacttatcg ttatgatcta caaaggccac cagcataaca aatccgtggt
     4441 cggtggcaaa aaaagcagat ttcgcttatt aaaaccacac attgattgaa tgtttgaata
     4501 aacgcgcgat tttttcaaaa agtttgttga cctcaggtca tgatttccct aaattagcgc
     4561 ccgttccagc aagacaggaa cgacaatttg gtgaggtgtc cgagtggctg aaggagcacg
     4621 cctggaaagt gtgtatacgg caacgtatcg ggggttcgaa tccccccctc accgccagat
     4681 tataagtgca tgttattgtt taacatgcgc ttttttattt ttccttccag acagtatgcc
     4741 atcgggtatg ccatctgaaa ccggtttata tgccccgcga tcatatctta tcggacaatt
     4801 tccgatcttt gagcatactg gcaaagctat actgccagca tatcaggata gtgttttgcg
     4861 ataatatcat tcagtctgtc aaccagttct ggttttctga acgtgatatg cgcggagccc
     4921 ttcttaaagt atctgatgct gaacatctca tcttcatagc tctctttacc ctgaacagac
     4981 tgaatgtgat catccagacg aagggtgatg tttccccggt tgtcggggat tggtttaccg
     5041 ctgaacaggt gcaacattct ttccagatcg gcgagtcggt cagcctgttg tccggtgata
     5101 agatgaaatc tatggtcacc cccgtttttg caacactgat ttttgattaa tggttggctt
     5161 gcttaaatct atccggcgtc tctgtgggca aggatgcccg cgccccgatg agttccgcac
     5221 ctgataatcc taaaaatccc ctcggcttca gtgagccatt tcatctgtta ggtttttaat
     5281 caggtcgatt tgccgttcat gtcatcaata atcagtcttc gcaaaaccag aaaggtaact
     5341 tgtatcttat tgtttaaaat aaactaattg agttatatca gcatctatat caggcctgat
     5401 attctttttt ctgagtagtg attgcccagg caattcgagc cagtttattt gccagagcgc
     5461 aggtaaccac attcgaatgc ttcttgttga gttgctcccg aacccactca gcaagttttc
     5521 catgcttgcg ttcaagtcgc attatgaatg agcg
//
LOCUS       DAJTSI010000202         5525 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000202 DAJTSI010000000
VERSION     DAJTSI010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.203"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(74..1339)
                     /gene="uidC"
                     /locus_tag="OO327_003738"
     CDS             complement(74..1339)
                     /gene="uidC"
                     /locus_tag="OO327_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR6020104.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(1378..2403)
                     /gene="uidB"
                     /locus_tag="OO327_003739"
                     /pseudo
     CDS             complement(1378..2403)
                     /gene="uidB"
                     /locus_tag="OO327_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            complement(2409..4112)
                     /gene="uidA"
                     /locus_tag="OO327_003740"
     CDS             complement(2409..4112)
                     /gene="uidA"
                     /locus_tag="OO327_003740"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR6020105.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            complement(4500..5090)
                     /gene="uidR"
                     /locus_tag="OO327_003741"
     CDS             complement(4500..5090)
                     /gene="uidR"
                     /locus_tag="OO327_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR6020106.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(5319..>5525)
                     /locus_tag="OO327_003742"
     CDS             complement(5319..>5525)
                     /locus_tag="OO327_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6020107.1"
                     /translation="ALSEHKTLSVSWSHYPEIEQKMLQHTPIRRLGQPQDIANAALFL
                     CSPAASWVSGQILTVSGGGVQELN"
BASE COUNT         1442 a         1462 c         1234 g         1387 t
ORIGIN      
        1 gcatccggca taaaaaacgc gcactttgtc aacaatctga aacgccggag gtttttctct
       61 ccggcgttat ttcttacttc agcataaagt catactgaat actcattttc acatcccagt
      121 aattcaggcg atcgcccagc gtactgccat cgcgcttttc ccgcccttta tcggcagcaa
      181 gaaagaaatt catcccttgt aacggccctt cgctgacccg atagttcata ccacctaaga
      241 tacccgcgcc ttcaatccgc acatctcgtt tactggattc atagccgcga gtcacaatcg
      301 gtgcaaatgt cagcgtaaat tgcggagtta cacggtagtt ggcacctaat tgccaggact
      361 gcattccttc acggttacga tcaagactta atgaaaaggg atagccaatg tcggtatcca
      421 ccacggcacc attcattgca tggcgcaaat aaccaccact gccaaatacg cttccccacg
      481 gcgcattcca ggtcaattgt ccactaacca acgccgtttc attgggctgg ctggtattac
      541 gacttaaacc ttcgagctgc gcgtaaaagc cgagcaactc ccctttcaac gtataattcg
      601 ggtcgagcgg tatgcttttc tcgagaatac tttgtatctg attggaaaca tctttctgta
      661 ccgatgcccc cagagaatag cgactggtat catcatacca actggcggct aaaaaagaac
      721 gtgcgcgatg tttatcgtat tttcccgtct ggctgttata caccccttca tcacgaatac
      781 cggttgttga atcagtccat aaaaatactc gtgaacgcca cattccccac agatcaatat
      841 ttggcgttaa agcccaataa cctaatgctc cttcagaaac tgtcggtaat gtacgttgcg
      901 aactgccaac cattaacgga atacgataag gtaagctacc ataactataa tccgtcccga
      961 agcgacctaa tttaagcagg ctattttcac tgggttttat ttttactgcc cccagggcaa
     1021 agccaaaact tttatcacca tcaagatacc accgggtact catatcagca cgagcaccta
     1081 atttataaac ataatacgct ccgccttcaa cgccaacaat attggaataa taaccactat
     1141 tgaaatcgag caatcctccc tgtacccatg cgtaaatatc tcttcccgcg ccagcactgg
     1201 gcttatcagc cctgcgcaat tcgtttttca gtctgatgcg tagtcccgct tcatcgtcag
     1261 ccagttgcgc cgcgtaagca gaggtaaggc cagaggcagc cgtcaggcaa ataacggcca
     1321 tggccactat ttttctcata gttgcacctt tgatgttctg attattttta ttgaatatta
     1381 attagtgata tcgctgatta attgctgctg cactttttta cgattatcaa tttcaaccac
     1441 gatttctttg aattttttat ccgtgagcgg ataaaaccag ataataacga atgccagtag
     1501 cataaatccg caaggtacta aggcaattga tgtgcggatg cccataataa cttccggcgt
     1561 ttacacctga ttggcgatat atccgcttaa tcccaaaata aaggcaggaa ttgaacctcc
     1621 gattgcctga ccgcatttac gggtaaatga gaatagtgaa taggtgagcc cttcaattcg
     1681 cacgccggtc aggtattcac cgtattctac ggtatcagct tccagtgccc acatcacagt
     1741 catagtaacg ccctgaccaa ttgaagcgat ggccaacgca accaacgcca ccggcagtga
     1801 ccagacggaa acccagaaga atagcagata accgcaggtt cccagcaaag cgccaatcag
     1861 gaaggtattc tttttaccga tcctcgcgac catccccggc acaagcggtg ccgatgccac
     1921 agtaccaacc aggttttgca ccagtaccag cacagtgaac agcccggtat catttaacac
     1981 atagcgcacg tagaacaacg acgaggcgct gaccgcgaag gtcgaaatca gcacacacag
     2041 cgcaccgatg cacaacataa acagcgggcg attccgtttc agggtttgca aactgatctt
     2101 caatgacggc tgcgccacga tacgtaccac attttcacgc gtcgatttga agcagatgaa
     2161 gtaaagcacc attccggcaa tcaccagcac aatcgtccag aaatggtata ccgacaccat
     2221 ctcttccggg ctggagttct taatgctcgg tcctatcaga aatgccaggc atacaaaggt
     2281 caatgaagcg gcaatcccac gagccgcgcc cagacgggcg cgagattgtg gttgttgggt
     2341 catcgcggta gcaagtgaac cataaggaat attcaccagg ctgtagcaaa gcccgaggcc
     2401 catgtaggtc aattgttacc gccaacgcgc aatatgcctt gcgaggtcgc aaaatcggcg
     2461 aaattccata cctgttcacc gacgacggcg ctgacgcgat caaagacgcg gtgatacata
     2521 tccagccatg cacactgata ctcttcactc cacatgtcgg tgtacattga gtgcaggccg
     2581 gctaacgtat ccacgccgta ttcggtgatg ataatcggct gatgcagttt ctcctgccag
     2641 gccagcagtt ctttttccag taccttctct gccgtttcca aatcgccgct ttggacatac
     2701 caaccgtaat aacggttcag gcacagcaca tcaaagagat cgctgatggt atcggtgtga
     2761 gcgtcacaga acattacatt gacgcaggtg atcggacgcg tcgggtcgag tttacgcgtt
     2821 gcttccgcca gtggcgcgaa atattcccgt gcaccttgcg gacgggtatc cggttcgttg
     2881 gcaatactcc acatcaccac gcttgggtgg tttttgtcac gcgctatcag ctctttaatc
     2941 gcctgtaagt gcgcctgctg agtttccccg ttgacagcct cttcgctgta cagttctttc
     3001 ggcttgttgc ccgcttcgaa accaatgcct aaagagaggt taaagccgac agctgcagtt
     3061 tcatcaatca ccacgatgcc atgttcatct gcccagtcga gcatctcttc agcgtaaggg
     3121 taatgcgagg tacggtagga gttggcccca atccagtcca ttaatgcgtg atcgtgcacc
     3181 atcagcacgt tatcgaatcc tttgcctcgc aaatccgcat cttcatgacg accaaagcca
     3241 gtaaagtaga acggtttgtg gttaatcagg aactgttcgc ccttcactgc cactgaccgg
     3301 atgccgacgc gcagcgggta gatatcacac tctgtctggc ttttggctgt gacgcacagt
     3361 tcatagagat aaccttcacc cggttgccag aggtgcggat tcaccacttg caaagtcccg
     3421 ctggtgcctt gtccagttgc aaccacctgt tgatccgcat cacgcagttc aacgctgata
     3481 tcaccattgg ccaccacctg ccagtcaaca gacgcgtggt tacagtcttg cgcgacatgc
     3541 gtcaccacgg tgatatcgtc cacccaggtg ttcggcgtgg tgtagagcat tacgctgcga
     3601 tggatcccgg catagttaaa gaaatcatgg aagtaagact gctttttctt gccgttttcg
     3661 tcggtaatca ccattcccgg cgggatagtc tgccagttca gttcgttgtt cacacaaacg
     3721 gtgatacgta cacttttccc ggcaataaca tacggcgtga catcggcttc aaatggcgta
     3781 tagccgccct gatgctccat cacttcctga ttattgaccc acactttgcc gtaatgagtg
     3841 accgcatcga aacgcagcac gatacgctgg cctgcccaac ctttcggtat aaagacttcg
     3901 cgctgatacc agacgttgcc cacataatta cgaatatctg catcggcgaa ctgatcgtta
     3961 aaactgcctg gcactgcaat tgcccggctt tcttgtaacg cgctttcgca ccaacgctga
     4021 tcaattccac agttttcgcg atccagactg aatgcccaca ggccgtcgag ttttttgatt
     4081 tcacgggttg gggtttctac aggacgtaac ataagggact cctcattaag ataaaaatac
     4141 tggtcaacct ttaatctgat tagttaacca attctgtgtt gcacacagtt atctgtgaga
     4201 aacccatgga agagaaatgg aaataaaaag aactgacacc gcgatcaaaa aaccaaaaac
     4261 tgcaataccc acagcgtgat atggatcgca ttaatcttta aaacagcgtt gacagaagcc
     4321 ataaaataag caatcgaacg ttcgttcgag agtgttggga acgataaact ctcatgacat
     4381 atggctacgg tgaatatttt gggggggaat tctccgaaca cggggaggat aatttttgca
     4441 cagagaaatt acgatatgaa acgcgggcac agttatcacc agcccgcgaa atgagaggat
     4501 caggatgcgg ttaatatacc gccaatcatc gcgcgtaatc cctgagcaaa cgacgcttca
     4561 cgcgccaggt cttccgcttc gatatcggcc agtacaccgt aggtcagatc cagcagtaaa
     4621 cgtgaagtca ttgccgtgtt gatgtctggg cttatctcgc ctttttgctg ggcatcacgc
     4681 atccgctggg caacaaattc agtgatcgtc atatgcttat ttttaagcat ggcggcgacc
     4741 tgtgggttac gcatcccttc cgccataatt tcgaccacca gcgcccgttg cccaaaggct
     4801 tcatgggtga gagagacaat agactcgacc atatagtcaa caaaatgaat cccttcaatc
     4861 ggttcccgga aacgagccag cgccctctcc tggtcctgta agataatcgc ctgaatcaag
     4921 gcttctttgg agatgaaatg gtgatagagc gtcccaggac taatggcgca agatttacag
     4981 atggctttca tcgaggcact gtgaaatcca ttttctgaaa aaatctctct ggcagcattg
     5041 aggatccggg tccgtgtcgg ttgtgcttca gtctgcatgt tatccatcat taaaccatta
     5101 tagttagcgt gaataactag tttaccgcgt tgtcaggtga gcgcctatca ttgctgttga
     5161 atagtgcgaa ggtacactct attcgtatac ttttcaccca gcaattggtt aaccaatagc
     5221 cgtgatttgg ctgccagttg tttagcagag acgttgatga attgcacacg ctatcccggt
     5281 aaacaacaca tacgaccgtt taccggttcg ttagtgtatt aattgagctc ctgtacccca
     5341 ccaccggaga cggtgagaat ttgtccgctt acccagctgg ctgcaggcga gcaaaggaac
     5401 agcgccgcgt tagcaatatc ttgcggttgg cccagacgtc tgatcggcgt gtgctgtaac
     5461 attttttgtt caatttctgg gtaatgactc caacttactg atagtgtttt atgttcagat
     5521 aatgc
//
LOCUS       DAJTSI010000203         5485 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000203 DAJTSI010000000
VERSION     DAJTSI010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.204"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1630)
                     /locus_tag="OO327_003743"
     CDS             complement(<1..1630)
                     /locus_tag="OO327_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCR6020108.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPW"
     gene            complement(1646..2530)
                     /locus_tag="OO327_003744"
     CDS             complement(1646..2530)
                     /locus_tag="OO327_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR6020109.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(2541..3146)
                     /locus_tag="OO327_003745"
     CDS             complement(2541..3146)
                     /locus_tag="OO327_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR6020110.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(3317..>5485)
                     /locus_tag="OO327_003746"
     CDS             complement(3317..>5485)
                     /locus_tag="OO327_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HCR6020111.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1581 a         1364 c         1141 g         1399 t
ORIGIN      
        1 cccacggttt accatcttta tgactggtta acaccccggc aatcagctcc agttgtgggt
       61 gccacggctg cccggtgtag atgagaatgc cacccggttc gatggcattg gctagacctg
      121 cgagtgagtt ttttacctgc tcattttcgg gaaaaagctc atataggcca gagacaatcg
      181 ccagcgtagg ccgcggagtt aacgcgctga gttcctccgg gttaaacgca tcgccctgtt
      241 caaaacgcac ccgcccagac attccccgtt gggcaatcat ctcttgcccc tgtgcaacat
      301 ttaactcact gtaatcacgt aacaaaatat cgcttacggc aggctcgttt gccagcgcat
      361 ccagtacata gcgtccatgc cctgcggcaa tgtcaaccac gcggacggct aaacctttgg
      421 cgtggagatc ggcaacggct tgtttaatca atatttgcaa atgggttttg cgctggcgaa
      481 taccgcgcca tccaacactg ttcaggtaga ttttgtcgat taaacgcccg aatgcgttac
      541 tgccttgcgg ttgattttga tagacataat ccagggaact gcctgaatca aagccggtac
      601 tcattccgag atgcaggccc gcagagtgcg caccgaacaa tttcatcgcc tttcgcataa
      661 agcgatacgc taaatcaacc ggcgataatg gcacgggtcc accagaaagg agccgccatc
      721 gatccgctga tggtcctgtg cggtcttcat gctgataatc aaatttttgc gatttgttag
      781 catataagcg gcagataaag ctttgcattt tttcaaatgc ctgcgccctg ttctcttcac
      841 ccaatgtgtc gtgataaaag cctggcagca gatgcagctc tttcagaggg ctacgtaatc
      901 tctgataaaa atcaatctgc ggttggcgat gcaccacata gtcatcacct gatatcagaa
      961 gttgcgtggg gagcgtaatc gccgccgcat cgctaacaat acgttcagac gttttataga
     1021 gatcgagcaa gatattgacg gcaatcgccc gtgtgatcag cggatcatta ttgaaactcg
     1081 ccacccgttg ccgatcgtgg gtcaaatagc gccctttcac ataggaatta ataaaaaata
     1141 gaccacgcag acgatgccat aacgccagcg caggacgtgc cagcggcaca tacaatttaa
     1201 ccttaaaggc cggagaagcc agcaccagcc cacgaattgc aggtgcataa tcatgaaccc
     1261 atgtggcaac aagcactgcg ccgacgcttt gcgcgatcac taccacctct tccagtccga
     1321 cctggctgtc gctggcagca aaacggacaa attcatcgac atcctgcact gagcgcataa
     1381 gagacgggct ataaccacgc ggccccgaat tttttccatg cccccgggca tcccaggcat
     1441 aaaaagcagt atcaggcatc gccagttcat caacgagatg ttgtagacga ccagaatgtt
     1501 catgcccgcg atgaaataag acgatgacct ttttcgcccc tggctgtaaa gtgggccagt
     1561 gccgataata caatgctgta ttatcactgg tggtaaaaaa atgttcccca gggatgcgtg
     1621 aattttccat tttctctatt ttccttcagt aacagcagta gcgaataaaa taaaaaaata
     1681 ccggagcggt gaaaattaat gagtcaatcc tgtcaaggag tccgccatgt cctagtaata
     1741 gttttccact atctttaaca ccaatatctc gtttgatggc tgacatcact acatcgccgc
     1801 aaaaaccact aataccaatt aacaaacccg ctaataatgc ctgtaatgta tttagcggtg
     1861 tcagcaacgg tccaataatc agtgacgcaa tcatggtggt gattacgccc cccagcagac
     1921 cttccagcgt ttttcccggg ctgactttgg ggaccacttt tcttctgccg caggatttcc
     1981 cccataaata ctgtgcaata tcgtttgact cggttaacgc caaaagaaat aacaccagta
     2041 acgccccgcc ctggatattt atggttggca acattaatag ccaggcggca tgactcaagg
     2101 caaaaacggt cattatccat ccccaaaaaa tggcgctcac ggtatgctga aaaccggagg
     2161 ggtcacccac aaaaactcgc caggtggcta atatcagaaa cccagtcaaa ggtataaata
     2221 acagaaacag ctcaaagcaa ttaaaaccga taagcaaata gttaagagga ataacccaat
     2281 ataaccaacg cgaaaaatgt acggatataa gcgtacaata ttctttcaat gccagaaaac
     2341 ttatcagcgt aaaaaacgtc aacgtcatcc agcgtgggcc tgaaatcacc agtgaaaaac
     2401 aaatgacgat gccccaccag gtacgaatac gtaatgtaac ttcatcccct tttctctcag
     2461 gtaaccgcca tagcagaaaa cggtttatca gcttgctaaa attaacaacg cgaaaagtgt
     2521 tgccagagat ttttccagca ttatctttca gcgctcataa gaagcacact ccgacaacga
     2581 ttaatcgcag tacagataag cagaattgaa gcaatgctcc ataaaagatt attccattgc
     2641 atccactgtg gataaatggc aacggccaga ccccacagac caaatattaa cgcgcgatca
     2701 cttttgccaa atggaccggc ataactgcga acaccgttaa tcgtctgcgc gagtaaaccg
     2761 caaaactcgg tcaatatggt gcaaaataac atgagtatca cgagtgatgc gttactttcc
     2821 ggtaaaaata aaaacggtaa atagagcgca atatcggaaa taacatcgcc agtctcattc
     2881 aaaatcgccc ccagacgtgt ttgctgattg cactcacgcg ccaacatgcc atccagcgca
     2941 ttgagcgcca tacggataaa aagcacgatg ggcaatagca aaaagaggat gggttgtgcc
     3001 accaacacca acagcaatcc ggtaaaaaga gaaagcgcca gtgccgtaag agtgatgtga
     3061 ttcgctgtaa cgtggtgctt ataaagccaa aacatcgtcg gccttaacag cgactgaaag
     3121 agcggtttta tctggtagag cgtcattatc aatccttgat aattcggttt gcgctatggt
     3181 gcatcaattc ccctgacgga gatatcgcaa cacgccgctt cattaaaaat tcagaatata
     3241 tcaaggtatt gggggctcac tgtcaggaat gattatcccc aataaatcaa gtagttaaaa
     3301 attattacgc tgttattcaa tacaactcat caagtttcct catcgcttgt tcccgtattg
     3361 cgcgcgtctt accgttaagc gccagctggc ggactaaatc cagttcaggg ttaacaagct
     3421 ctaacgccag caaaggatca cgtttcagcc tcgccttcaa tacatcaact ggaaagcggg
     3481 gattattcat cgccacaatc caccactcgg gatcaatttc tgccgccagt gccgcagagg
     3541 gggtgtggcg gttgctaagt gcgccgaact tgaaaaatgt gagattgctt ttcgccagtg
     3601 tactccacca tatacgatcc ggttgttttg ccagtacatc cagttgtgct aacgcatcgg
     3661 ggtcgagtgc ataataaaca cgtaatgctt catcaacctg cgcacgggag ctattttcct
     3721 gcctcagaaa aaacttcgcc caaatatcat tgcgtggcgt gttttgctgt aattccttga
     3781 gaacaacagc gggtaaatta tcccaactaa ctaatgcact ctctaaaaag tcccagtctt
     3841 ccaaacgtat cagggcggcg gcatttaata gcatctggtc atcaacttcg ccgtggctaa
     3901 tcagctcaga caaaatcata tcagcgctgg tttcatatgc atctgaatct gctttcggat
     3961 tatttttccg atgcgataaa gcacgttgcc aaattttttg tttcatcgac gtgctcagat
     4021 actgattttt cgccagatta ctccacccct caggatcgtt gaaacgtgtc aataaccgtt
     4081 gtaaagcagc gtcagagtga attacctgca tatcccgaca gcaaaagtag gcaggggatt
     4141 ttttctccat ccttttaaca aaattaaact gtaagttatc tggcaatgct aaaaataaca
     4201 cacgtattag atcatcgtca ttctcgaatt tttggtataa ataggctgca actttgtcac
     4261 tctcttgtgg cgtcatccgc aataattgac catggtgttt catctctgcc agcttgaacg
     4321 ctaagcttgt gacaacattg tttgatgaat cgttcgccaa ctgcattgcc tgattgtaag
     4381 tgagttgtcc tccagcaccg acctgacgaa tagaggaatc atcactgcgg atatagccat
     4441 caataatgga ttcgggcaat aaccacgcat ctttataaag ctcctgaatg atcatatccc
     4501 aatctctttt cgccagagtc atgacgatat ccagagggag attttcacgt agcgcaagat
     4561 tcaacggacc gacaccatca ggtagacgac tcattcgcgt cattatttcg acagagagcg
     4621 gttcacggtc gagcaacccc agaacaacag gctggtaacc caattgctga tcggtcaact
     4681 ccagaatctc attgtcaggc atatatctcg ccatactttc aatcgtttgt tcatcattgc
     4741 tattaataag ttgtttgcgt aggttagctg aaattccttt aagccttggg agagttcgca
     4801 ttatggcaag atcaaatgtc gcaatatctc gtccttcaaa tgcagcaacg ttgccttcat
     4861 ccagcatttg caattcctgc actaactcag gagaaggctt agggcgaaac agcgcttctt
     4921 ttttcatctg attacagaca ttccctgcca tgcgaatatt gcttcgctta aaaatttctt
     4981 tcagagcatc ttcatcattt attagacccc aataatatgc atcaggattt attatcatca
     5041 gcgctttttt gacattttca gtgtcatttt tgtcactcgc acggaccatg aattcactat
     5101 ttgtcgaaga cgcaacaaca ggtgcaatta tgtcattgat aaatacccca cgcgcattaa
     5161 tactcccctt aatcgtagac ggattatcgt aatatgagtc gataaatacc agtccacttg
     5221 catttatcga accggtaatt aacagtggtg aatccgtaac tatattcgtt gccgtcacgt
     5281 taccgagaat aacccatgga ttagaaagag agaaagtgga aatattacct ttgactgtca
     5341 aattgccaag aacaatcaat ttttgattac tctctatatt accttctacc actaaatcgc
     5401 cacgatgcac tttccagtcc gatgcagata aaccgaaggg gacaagattc acgctactgt
     5461 tgatagcttg ttcaacttgt tgttt
//
LOCUS       DAJTSI010000204         5398 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000204 DAJTSI010000000
VERSION     DAJTSI010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.206"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..497
                     /locus_tag="OO327_003747"
     CDS             <1..497
                     /locus_tag="OO327_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HCR6020112.1"
                     /translation="EIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHL
                     IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEK
                     LAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHA
                     ESAH"
     gene            519..2510
                     /gene="cyoB"
                     /locus_tag="OO327_003748"
                     /pseudo
     CDS             519..2510
                     /gene="cyoB"
                     /locus_tag="OO327_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2500..3114
                     /locus_tag="OO327_003749"
     CDS             2500..3114
                     /locus_tag="OO327_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCR6020113.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3114..3443
                     /locus_tag="OO327_003750"
     CDS             3114..3443
                     /locus_tag="OO327_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCR6020114.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3455..4345
                     /gene="cyoE"
                     /locus_tag="OO327_003751"
     CDS             3455..4345
                     /gene="cyoE"
                     /locus_tag="OO327_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR6020115.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVCGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4529..4840
                     /locus_tag="OO327_003752"
     CDS             4529..4840
                     /locus_tag="OO327_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6020116.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4824..4982
                     /locus_tag="OO327_003753"
     CDS             4824..4982
                     /locus_tag="OO327_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR6020117.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAT
                     RIAKEKCA"
     gene            4975..5373
                     /locus_tag="OO327_003754"
     CDS             4975..5373
                     /locus_tag="OO327_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6020118.1"
                     /translation="MRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQP
                     SEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFY
                     ARLGFVQLSATPNAFGAVPMFLAIQHILAA"
BASE COUNT         1176 a         1344 c         1426 g         1452 t
ORIGIN      
        1 atgaaatcgc tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga
       61 tgaactcctt cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga
      121 ctcgcctgca tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca
      181 gcgggccggg cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat
      241 tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaacac catgtctgac atggctgcgt
      301 tcgaaaaact ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac
      361 cagacttgtt tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga
      421 cccagccaga aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg
      481 cggaatccgc ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg
      541 atgcagtccc gttccatgaa cctatcgtca tggttacgat cgctggcatt attttgggag
      601 gtctggcgct cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt
      661 ggctgacctc cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga
      721 tgttgctgcg tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg
      781 cgggtgaagc gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg
      841 tgattatgat cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc
      901 cgctgcagat cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt
      961 ttactgttgt tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga
     1021 ccggctggct ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg
     1081 attactggat atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact
     1141 tcttcgttac cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat
     1201 ttacctaggc atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg
     1261 ttaccgtcgc gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata
     1321 tgggtggcaa catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt
     1381 acatcctgat cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta
     1441 aacgtctgtt tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt
     1501 tcatcgtttg gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct
     1561 ttggtatcac cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt
     1621 tcaccatgta tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta
     1681 tcgtcacctt ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact
     1741 tcgttctgca taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg
     1801 tggtcttcgg ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac
     1861 tgaacgaaac ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct
     1921 ttatgccgct gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg
     1981 acccgcagtt ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg
     2041 gtattctctg cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg
     2101 acctgactgg cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc
     2161 cgttctataa ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga
     2221 aagagaaagg cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa
     2281 acagcggtgc aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct
     2341 ggcatatctg gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga
     2401 aaagcttcga cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa
     2461 accagcattt cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg
     2521 cacgcgactg cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc
     2581 ggattttgga tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat
     2641 gccgttctgg tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg
     2701 ttcgttctgg ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct
     2761 atcgccatgt acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg
     2821 tttggtgccg gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc
     2881 atggggccgg atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt
     2941 ctgcacgtca cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc
     3001 ggcctgacca gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg
     3061 gatgtggttt ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc
     3121 attctaccga tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta
     3181 tcctgtcgat cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc
     3241 cggccgtaat tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg
     3301 tgtgcttcct gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct
     3361 tcaccgtgct aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca
     3421 actacaacat gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt
     3481 aacgaaacca ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc
     3541 ctcaaagggc agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt
     3601 tgtggcgtcg ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga
     3661 aaggacgaag aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta
     3721 cgccaccttg ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc
     3781 ctgctggctg ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa
     3841 acgccactca gtctgcggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat
     3901 tggctactgt gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt
     3961 cagcctgtgg cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca
     4021 ggcggcaaac attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat
     4081 cacgctgtat atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc
     4141 tgggtataaa tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct
     4201 gcgcggttat aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat
     4261 catcgccatc actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca
     4321 tacgctgctg gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct
     4381 ttttattgtc tgagatcaga gatacggcta ataaccgtat ctcttgacag agtgagcaga
     4441 tgcacataaa ttaatcatac ggttgatagc cgtattatgt atgagatcga ctccctacga
     4501 tttcattcag ttaatcaaag aagtcataat gcctggaaaa acagccgctc tatacgatgt
     4561 cgataaaacg ctgaaaaatg cgcgggtcga actcaaaact agtcccgacg ccaaaaacaa
     4621 acttcgtgaa gctgcgcaag ccgttggggt agatttaagc gcttttattt tgagcgctgc
     4681 tatggaacgc gccgaaagtg tgcttgataa ccaacgtcgc cgtgagcttt cgaatcaaag
     4741 ctgggaactg atgaaccaac tcatcgctga acctgctcaa ccgacgctcg ccctcaaggc
     4801 gttaatgaaa aggaaaaaca gcgatggtcg acaagcatga agagattact ctgcccatag
     4861 tcctctcctg taattatcag tctgatatta cttatcctgg gcaaaaacag tttgattgcg
     4921 gtaaccctgt tatcgataaa tttgtacgcg ctacgcgcat cgctaaagaa aagtgtgcgt
     4981 aatagcgact gtgcggctaa agcacttatt gacagacaaa gtggtgaact gattggcatc
     5041 tgtactttta cggcatattc gctggaaaga caacgcgttt ctggcgtcct tcagggttca
     5101 caaccttcag aaattggtgt tgtcagatta gtcatgttgg ggggagcgcg gaagtatcaa
     5161 aagcggggct ttggtcagga cctactatgt gatttttttg aacatgtaaa aaaaattcac
     5221 ccggcattac caattaaagg ggtttatctt gatgctgacc ctgccgccat taacttttat
     5281 gctcgtctcg gctttgttca gctttcagcg acaccaaatg cttttggtgc tgtacctatg
     5341 tttttggcga ttcagcatat tctcgcggct tagattatta tcgccacccg ccagggca
//
LOCUS       DAJTSI010000205         5350 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000205 DAJTSI010000000
VERSION     DAJTSI010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.207"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(75..557)
                     /locus_tag="OO327_003755"
     CDS             complement(75..557)
                     /locus_tag="OO327_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020119.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNVKTSADNDVD
                     LDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIGVFDFANNASPATMTFLIPIYKD
                     DDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVNVNGESGVTYENCKLNMKDINDD
                     "
     gene            complement(625..2574)
                     /locus_tag="OO327_003756"
     CDS             complement(625..2574)
                     /locus_tag="OO327_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6020120.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2659..2994)
                     /locus_tag="OO327_003757"
     CDS             complement(2659..2994)
                     /locus_tag="OO327_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR6020121.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2994..3350)
                     /locus_tag="OO327_003758"
     CDS             complement(2994..3350)
                     /locus_tag="OO327_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR6020122.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(3350..4846)
                     /locus_tag="OO327_003759"
     CDS             complement(3350..4846)
                     /locus_tag="OO327_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR6020123.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVEKYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4843..5004)
                     /locus_tag="OO327_003760"
     CDS             complement(4843..5004)
                     /locus_tag="OO327_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR6020124.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(5014..>5350)
                     /locus_tag="OO327_003761"
     CDS             complement(5014..>5350)
                     /locus_tag="OO327_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020125.1"
                     /translation="RDEKGQCAAYDAVHDVRRELWKALLGWMPDPQGGEIVYVGGTLL
                     DLNRYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLSIDVDYIDPGTGPDGDIEH
                     HLEMRFPQN"
BASE COUNT         1277 a         1559 c         1150 g         1364 t
ORIGIN      
        1 aaagagaagg taaagaaggt aagtccatac tgtctccact ataaaaaaac cgccaatttc
       61 tggcggctta tatattaatc atcattaata tctttcatat tcaatttgca attttcgtag
      121 gtcactcccg actcgccatt aacattaact ccagctctga tattcccgtt ttcttttaca
      181 tctacaaaaa actggcctct tggcatcttg aacgcaaatg taaaaccaac agtatctgaa
      241 tcaaaatcat catctttata tattgggatc aaaaatgtca tcgtcgctgg tgatgcattg
      301 tttgcaaaat caaatacacc tatacttttg ccgaattcat aagaagcttt tctcaatata
      361 atcgcaccgc tggcagaagg ggatgggcac gatatcctca gttgtctgaa tccatctgta
      421 tccaagtcaa cgtcattatc tgcagatgtt ttaacgttac taataaatcg tgaaacaatc
      481 tcctcatttg ttagtatttt cgcctgagca cagcaagagg ttaatattgc aaccgtcatt
      541 aaaaatggct ttatcacaaa acaaactcca ttgttagcat gttacgcgat aattttgcct
      601 catctttcac cacaaaaaaa gaactcacct tttaaatgga gaatacccaa catcatgggt
      661 gattttcata aagggatcgc ctgttttcgg caaatcgata acgcgcattc caggcggcgc
      721 gttgtcaaac gtcaccttaa gttcgctgcg ggttgccggt gaagatgcaa ggcttaacag
      781 ctgattcctt tccagtgata cattcggggt gtagcgttta tcgggagata aatacccatc
      841 accacgtggc atctgccatc cggtcatgtc atagacaaaa tcatggaact gtttgcccca
      901 ctcgtcgaac ttatcattca gaccaaatcc actgttcagt gcatcagcca caaaattttt
      961 aacaagccag ggatgttctt tttcaaactc ctgcgccatc atgcccagtt caagcaatcc
     1021 accaatcaga ctgatctttc cgaggccctt taatttcagc cctgtttttc ctgccgccgt
     1081 tgtccattga ttctgggcga ctgttgccgc acgaatactt gccaccgcct tcacaccaat
     1141 atacagggca gaaaccgttg ctattgtttt tactgcctgt tcccatcccc ccattgctct
     1201 gacaacctga tcaacctcct gccagacttt tttcacaacc ggaccgaccg tttcccagtt
     1261 atcaattatg agatatgcac caccgacgag aagcgcaatt aatccctttg ccggcgtcat
     1321 attcatcacg ctacccatga tttttgttat gcgtgtcaat gtgccaatcg ccacgcccat
     1381 cgtcagtaat gccgcaccag ttttcgcgat ggtttttacg acctcaggat tttctctgac
     1441 aaacgtgcgc acttcttcca gaaaaggctt catctctttt atgccttcat tgagtgaagg
     1501 caggaaggtt tcccccagcg tggaagaaat cgcattgatc tggttctgca gcagcaacaa
     1561 ctgattttct gtcgtcgcgg cacgggcggc atattctttc tgcattgagc cgccatactg
     1621 ctgcgcatcc gccacacgat taaagttggt tcgcaacaaa tccagattcg tgagcagcgg
     1681 cgcaatggcc cccagggatt ccttcccgaa cagcgctttc agcacagcag cctgtttttc
     1741 tttcggcact tttgccagag aatccagtac gtgcagcatg gccccgcggg cgtctttctg
     1801 catatccgcc gccagttttt tcgggctaat gcgcaacgag cggagcactt ttttctgcga
     1861 tttggtggca gaatccctcg ctgtcagcga caacataaaa ttctttatcc ccgtcgcagc
     1921 aatttctgac tccaccccca ttccggcaat ggttgccccc attgcggcaa tctctccgga
     1981 ggccacaccc gcaacactgc ctaaagggcc aatacgggtc acaacatcag aaatcttttt
     2041 cgcacttgca ggaccggtat tgccaagata gttaatctta tccgcaagcc ctgccacctc
     2101 tccctgtgtc agtttaaagg cagtgcgcca ctgcgccatc atctgaccgg attcttctgc
     2161 cgtggtgtca aaggccacgc ccatcttcac ggcatcgtca gtaaactgca tcagctcgtc
     2221 acgggcgata ccagcctgac cacccgccgc cacaatttcg gcgatacctt ccgccgacat
     2281 gggcagttct gttgacaggt cgcgcacctg ctccgtcatt gccttaaacg cttccggcgt
     2341 atccagacca tccaccactt tccggacatc cgccattttt gattcaaggg caatggcaga
     2401 tttgaccggg agcgccagcg cccccagtac tgcggttccg gcacccgctg cgcccagtga
     2461 caggctggca aactcctttt taaaaccttt cagctggcgc tgcattccct tcagcggtgc
     2521 cgatacctta tccacggcag tgatgatcgc cttcagctga aaactgtcag ccatgcttca
     2581 tctcctcgtt aatgcggacg gcctcggctt ccagttctgt aaactgcgaa acagccaccc
     2641 gtcgcagctc cagtggattc agtttccaga accaggcaac attgtagagt cgtttccgga
     2701 ggtgcttccc gtctccgact gggtaaaaaa acgcaggatc tgcatgctgg tcttaaaaat
     2761 atccagtttt gccatctgcg ccgccgatga acacgggatc cccgccagca acgggatata
     2821 tttcagtgcc acctggctgt ccagtttaat actgccttca ccggaaataa tgaaaggaaa
     2881 acccagcgcc tcgatttcgt catacgtggg ctcgcgcaac tccagcacat gcagcatttc
     2941 gttatgcgct gtcaccggtt tttttaaaac tatttctgta acgttcattg ccattactga
     3001 taccctccct cttcgccgtg gaattcaaga tctgccgtgc cttcttcggc attatgatta
     3061 gcctctccgt gcagccatgc cgccgaaagc acatacacca tgccgtttgc cagctcagcg
     3121 gtaatcgtca tctggtctga ggtggtaatt ttgtcgaccg gaaagttttt gggcacttta
     3181 aacgtgccct taacgtaagg tgaccgccag gtctccttgt aatccacatc ccctgccatg
     3241 ccgacgatgt catcccggac attggtgttc atcggcactt caatgccacc tgtcagcgat
     3301 aactgctgac catccacttt aaaaaaacag gttccggcga tctttgccat tatgctgact
     3361 cctctgaata ctgaagacgg aactggttaa ccaccgcaaa gacacgtaac tgattaacat
     3421 aatccggcgg gaacagcgta ttcagtcggt tcgggttatc tgcatcacgc tcaactatca
     3481 gatactgttt aaacagatcg tatttttcca caataccggc acgctccatc tgacgatatg
     3541 tcgccagaag ttcccctttg ataacggcag gagtgacaat cgcctgcccc ggaccaaaac
     3601 gggtaccatc atttgccagc ttgtgacgtc cgtacttgct tgtgatgacc gatttcagtt
     3661 tgcgaagaac atatgcactg gtatgcagag tttcactgtc cagatagctg ttgtctgcca
     3721 cgccatacgc attctttttg taggtggtta cggaacgctg gatctgcaac gtgccgcctt
     3781 ccacataagc cgtcgccacg ccgtgagata aaagggtctg ctgctctgtc atgatgaatc
     3841 gcttaccttt cggtgccgga agcatcccca ccagctcccc cgtctgtgtc ggacgggcag
     3901 gatcattccg gataaatacc gcttcacggg caaggcgact ggcaaccagt tcatcgacag
     3961 gcgactgggt ttctttttcg taaccggcaa gcgtgatatg ttgctgatta tgcatatctc
     4021 cggcaccaac cagctctgac agcgttccca gttttgcggt atagacatgc ccgtataact
     4081 ggcgcgcata actccagcga ccgctgctgt cattcatttc ggtcatcatc atattgatgg
     4141 aggcggcatc gttgaacggc agaccgataa agtcgaatgc ctcatcgccc atagcagcaa
     4201 cagcggcggt aagatcaggc gctccgctac cggcagcacc gacttccgtc accacccgaa
     4261 gccctgctgg caaaatctca ccaccaccag aaccataata attcagacag accggtaact
     4321 cattaccata cagcccctta tgacgggcag tcagcgtcac cacgcctgca tcagatgacg
     4381 ccgtaaacgg cagggtgata accccgttta ccgcttcctt aatcgcggtg gcaaccgcag
     4441 tggcatcatc gccattcacc acaggcacct gtacacggga gcgcccgaca tacagactca
     4501 gggtgccgct ttcctctgct tctccggcaa ccgtcaccct gaccgtcgcc gccgcccctc
     4561 tggcttccgg tactgcaata acatacagtt caccgaaagg atctgtctga cggtagactt
     4621 ccaccatacg cgccagctgg ctccccgccc cacaaatctg gcgggcataa tctgccgatg
     4681 gcatcagcac caggctgtta acctcaatgg ctgcatcgtt gctggcatgc ccgatcagta
     4741 atgcaggcgc gctggtcacc gctgtatttg ccgcagaatt gttcatctcg gcgtagaaca
     4801 acggcaccag cgtattcgac ggaacagtat taaaacttat agtcatgatt ttttagcctt
     4861 atcctgttta acacgtacca catcacccgc cgctatacgg cggaaccagt agctgctttc
     4921 ttccacattt cgcccttcgg aaggcaaaag gtctccacgg gcagggtcag gaaccgaccg
     4981 ccctttcagg ggtttcacaa acatgaggga ctcttaattc tgagggaaac gcatttccag
     5041 atggtgctca atgtcaccat ccgggccagt accaggatcg atgtaatcca catcaatact
     5101 cagcagggat aaatccggca gggcattcac gtcctctgcc tgtcgcgtgt cttcttccgt
     5161 gatttcatac ttcgccgtaa aatcaaactg gtaatacagt tcgtaacggt tcagatccag
     5221 cagggtgcca ccgacataaa caatctcgcc cccttgcggg tccggcatcc atccaagcag
     5281 ggctttccag agttctctcc ggacatcatg aacggcgtca taggctgcac actgcccttt
     5341 ttcatcacgc
//
LOCUS       DAJTSI010000206         5338 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000206 DAJTSI010000000
VERSION     DAJTSI010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.208"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="OO327_003762"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="OO327_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR6020126.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="OO327_003763"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="OO327_003763"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR6020127.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="OO327_003764"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="OO327_003764"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR6020128.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="OO327_003765"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="OO327_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR6020129.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1310 a         1289 c         1458 g         1281 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agcggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctatg
      181 cggttaacga cggcaaccca tgcgccacgt tctgtatagt actcggccac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgcgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc acccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgaa taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggtaaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc cctctggata tcgtctatga agatgaagat atcatcgtca ttaataaacc
     1201 gcgcgaccta gtggtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattac tatccgccca ttgccgatgt accacgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc actggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctccggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc gctgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgtggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcaacag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat cgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc agcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga tgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgaa tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaatattc tgttgcaggt actggatgat gggcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tctgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggttagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcgggg
//
LOCUS       DAJTSI010000207         5181 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000207 DAJTSI010000000
VERSION     DAJTSI010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5181
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.209"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..746
                     /locus_tag="OO327_003766"
     CDS             <1..746
                     /locus_tag="OO327_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6020130.1"
                     /translation="VDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWN
                     NVTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIAT
                     LGSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKL
                     SFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPL
                     GLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1080..3359
                     /gene="ydeP"
                     /locus_tag="OO327_003767"
     CDS             1080..3359
                     /gene="ydeP"
                     /locus_tag="OO327_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR6020131.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKNSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3607..3810
                     /gene="safA"
                     /locus_tag="OO327_003768"
     CDS             3607..3810
                     /gene="safA"
                     /locus_tag="OO327_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR6020132.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3878..4639
                     /gene="ydeO"
                     /locus_tag="OO327_003769"
     CDS             3878..4639
                     /gene="ydeO"
                     /locus_tag="OO327_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR6020133.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            5041..>5181
                     /locus_tag="OO327_003770"
     CDS             5041..>5181
                     /locus_tag="OO327_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR6020134.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDF"
BASE COUNT         1480 a         1102 c         1156 g         1443 t
ORIGIN      
        1 ttgtagactt gtctcagcat ataagttgct ggaatgatta cggcggctgg tacgacactg
       61 atcatataaa cctggtacaa ggctcagctt tcgcaggctc cctacagtca tataaaggat
      121 cgctgtactg gaataatgtg acctacccct tcccgttgac gacaaatacc aacgtattgg
      181 atattggtga taagacaccg atgccattac cgttaaaact ctatattacc ccagttgggg
      241 cggccggtgg agttgtcata aaagcggggg aagtcattgc ccgtattcat atgtataaaa
      301 ttgcaacatt aggaagcgga aatccgcgta attttacgtg gaatattatt tcaaataaca
      361 gtgttgttat gccaacaggc ggctgcaccg ttgatagtcg taatgtcacc gtggatctcc
      421 cggactttcc tggaagcgca gaaatacccc ttggcgtata ttgctcaagc gaacaaaagc
      481 tctcatttta tctttcgggt gccaccactg acagtagccg acaagtgttt gctaacacag
      541 cacctgatgc aacaaaagca tcgggagtgg gtgtcactct aatgcgtaat ggaaaaatac
      601 tggctacggg agaaaatgtc tccctgggga cagtaaataa aagcaaagta cctctgggtt
      661 taagcgcaac ctatggacaa acaggaaaca aggtttcagc cggaaccgtc cagtcagtca
      721 ttggtgtcac ttttatctat gaataattgt gattttgacc ttagtctatg cgctccattc
      781 acgaaaattg cgcggataat tattttgtga aggctattat cctacacctg taagaaaatc
      841 cgcgcacaat tgaagccaga gtcttatttt ttatattgga aaatatgtta atttaaacac
      901 tatgagagat gatgcttaac ttatcgtatt taatctattg tttaacgata atagattaaa
      961 aatagaaaga gagtagtccc ttactcttat atatccatgt tggcgataat ccctttgtat
     1021 gtactgtgca tcatcgctat tacaaatcct aataattcat ttccacacag gataagtaga
     1081 tgaagaaaaa aattgaatcc taccagggtg ctgcaggtgg ttggggtgct gttaaatccg
     1141 tagcgaatgc agtacgtaag cagatggata tacgccatga tgttattgcc atgtttgaca
     1201 tgaataagcc agagggcttt gactgtccgg gttgtgcatg gccagatcct aagaacagtg
     1261 cgtcattcga catttgtgaa aacggcgcaa aagcaatcgc ttgggaagtc acggataagc
     1321 aggtaaatgc ctctttcttt gctgagaata cggttcaatc attacttacc tggggagatc
     1381 acgagcttga ggctgcgggg cgtctcactc agcctttgaa atatgatgcc gtcagcgact
     1441 gttacaagcc attaagctgg caacaagctt tcgacgaaat tggcgcacgc cttcaaagct
     1501 atagtgatcc caatcaggtt gaattctata cttcgggccg cacttccaat gaagctgcct
     1561 ttctttatca gctttttgcc cgtgaatacg ggagcaataa ctttcccgac tgctccaaca
     1621 tgtgccatga accgacaagc gtgggtttgg cagcgagtat cggtgtaggt aaagggaccg
     1681 tgttgctgga agactttgag aagtgcgatt tagtcatttg cattgggcat aaccctggta
     1741 caaaccaccc tcgcatgctg acttcgttgc gcgctttagt gaaacgggga gcgaaaatga
     1801 tcgccatcaa tcctctacag gaacgtggcc tggagcgatt taccgcaccg caaaacccgt
     1861 ttgaaatgct gacgaactct gagactcagt tggccagtgc ctactacaac gtgcgcattg
     1921 gtggcgatat ggcgttgctc aaggggatga tgcgcctgtt aattgagcgc gatgatgctg
     1981 caagtgccgc aggtcggccc tcattgcttg atgacgaatt tattcaaacg cataccgtcg
     2041 gctttgacga gctacgccgt gacgttctca attccgagtg gaaagatatc gaacgtattt
     2101 ctggactaag tcagacacaa atcgccgaac tggctgacgc atatgccgct gccgaacgca
     2161 ccattatctg ttacggaatg gggatcactc agcacgaaca tggtacccag aacgtgcagc
     2221 aactggtcaa tttgctgttg atgaaaggta acattggcaa gcctggtgcg ggtatctgcc
     2281 cactacgtgg tcactctaat gtacagggcg accgaaccgt cggtatcacc gagaaaccgt
     2341 ctgcagagtt tctggctcgc ctgggtgagc gctatggctt ccccccacct catgcacctg
     2401 gacatgctgc aattgccagc atgcaagcaa tatgtacggg gcaggctcga gcattgatct
     2461 gcatgggggg caactttgca ctggcaatgc cagatcggga agcgagcgct gtaccgttaa
     2521 cgcaattaga tttggcggta cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg
     2581 cacggcatag ctatattctg ccggttctgg ggcgtagcga gattgatatg caaaaaagcg
     2641 gtgcgcaggc ggtaaccgtt gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt
     2701 taaaacccgc cggtgtaatg ctgaaatcag agtgtgcagt ggtcgcggga atcgcgcagg
     2761 cagcactacc ccagagcgtg gtagcctggg agtatctggt ggaagattat gatcgcattc
     2821 gcaatgacat tgaagctgtg ctgccagagt tcgccgacta taaccagcgc atccgtcatc
     2881 ccggtggttt tcacctgata aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta
     2941 aggctaattt cattaccagc aaagggctgt tagaagatcc ctcttcagcg tttaacagta
     3001 agctggtcat ggcgacagta cgcagccacg atcagtacaa cacgacgatt tatggtatgg
     3061 atgatcgcta tcgaggggta ttcggtcaac gagatgtggt ctttatgagt gctaaacaag
     3121 ctaaaatttg ccgtgtaaaa aacggcgaaa gagttaatct tattgcgctt acgccagacg
     3181 gtaagcgcag ctcacgccgc atggatagat taaaagtggt catttaccct atggctgacc
     3241 gctcactggt gacctatttt ccagaatcga atcacatgct aacacttgat aaccacgatc
     3301 cattaagtgg tattcctggc tataaaagta ttccggttga attagaacca tcaaattaat
     3361 gcctcttctc atttcttctg ctgtcatccg cacagcagaa gaattcctca ttgactatta
     3421 ttgcgcaatt tgctcacatg gattaaatta aactacatac tataagatat aaacttctgc
     3481 ctacagctgt aagaaactcc gctcagtact gaagcaccag tcctatttct tcttttctcc
     3541 agcctgttat attaagcata ctgattaacg acttttaacg ttatccgcta aataaacata
     3601 tttgaaatgc atgcgaccac agtgaaaaac aaaatcacgc aaagagataa ctataaagaa
     3661 atcatgtctg taattgtggt tgtcttatta ctgacactta cgttgatagc cattttttcg
     3721 gcaattgatc agctgagtat ttcagaaatg ggtcgcattg caagagatct tacacatttc
     3781 attatcaata gtttgcaaga ctggaaataa acagcaaatt ataaatgtga aatgtttaaa
     3841 aggtatcgat aaaactttat tgttttaagg agataaaatg tcgctcgttt gttctgttat
     3901 atttattcat catgctttca acgctaacat tttagataaa gattacgcct tctccgacgg
     3961 cgagatcctg atggtagata acgctgttcg tacacatttt gaaccttatg agcggcattt
     4021 taaagaaatc ggatttaatg aaaataccat taaaaaatat ctacaatgca ctaacatcca
     4081 gacagtgacg gtgcctgttc ctgcgaagtt tttacgtgct tcaaatgtac cgactggatt
     4141 gcttaatgaa atgattgctt atctcaactc ggaagaacgc aatcatcata atttttcaga
     4201 acttttgctt ttttcttgcc tgtctatttt tgccgcatgc aaaggtttca ttacactatt
     4261 aactaacggt gtgctatccg tttccgggaa agtgagaaat attgtcaaca tgaagctggc
     4321 gcacccatgg aagctgaaag atatttgtga ctgcctgtac atcagtgaaa gcctgttgaa
     4381 gaaaaaacta aagcaagagc aaacgacatt ctcacagatt ctattagatg ccagaatgca
     4441 gcacgcaaaa aatttgatac gcgtagaagg ttcagtcaat aaaattgccg aacaatgtgg
     4501 ttatgccagt acatcttatt ttatttatgc gttccgcaaa catttcggca acagtccgaa
     4561 gagagtttct aaggagtacc gttgtcaaag gcacacgggt atgaatacgg gcaacacgat
     4621 gagtgcttta gctatttgat tatttgctaa cgagtagtca accacgcacg ctacgtaaga
     4681 attatatggg gcagccattc cctgccccgc gttgttttta ggcgatatat ttattgaaat
     4741 aaataagtga catccatcac atatttatgc acttgcataa cctgttgcat gattatttat
     4801 gatctcaatt cggcattttg tcagtaaaat gcaataattt attaaatatc aataaattag
     4861 ttgtttatcg gcgagaaatt acttaataga acagaaagta atgtcaacgc tttatggact
     4921 gttatttccc tttttttagc taaatctgct atctctttat gtgactaact tcacttacat
     4981 ccacttattt ctcttcgtaa aattacttcg gaattaagta caataagaag aggaacattt
     5041 atgaagtctg cattaaagaa aagtgtcgta agtacctcga tatctttgat actggcatct
     5101 ggtatggctg catttgctgc tcatgcggca gatgatgtaa agctgaaagc aaccaaaaca
     5161 aacgttgctt tctcagactt t
//
LOCUS       DAJTSI010000208         5163 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000208 DAJTSI010000000
VERSION     DAJTSI010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.210"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..539
                     /locus_tag="OO327_003771"
     CDS             <1..539
                     /locus_tag="OO327_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HCR6020135.1"
                     /translation="IIDFSVPPTAYGKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLG
                     NGHTSCIFQESCKDNLVVESNGDIYECDHFVYPQYKIGNINKSELKTMNSVQLTAQKK
                     RISAKCQQCAYKPICNGGCPKHRITKVNNETVSYFCEGYKILFSTMVPYMNAMVELAK
                     NRVPLYHIMDVAKQMENN"
     gene            complement(505..627)
                     /locus_tag="OO327_003772"
     CDS             complement(505..627)
                     /locus_tag="OO327_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020136.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            830..2515
                     /locus_tag="OO327_003773"
                     /pseudo
     CDS             830..2515
                     /locus_tag="OO327_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            2553..4913
                     /locus_tag="OO327_003774"
     CDS             2553..4913
                     /locus_tag="OO327_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR6020137.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            4970..>5163
                     /locus_tag="OO327_003775"
     CDS             4970..>5163
                     /locus_tag="OO327_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020138.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGS"
BASE COUNT         1486 a         1118 c         1116 g         1443 t
ORIGIN      
        1 gtatcattga tttttctgtg cctcccacgg cttatggcaa gtttatgtca accattttta
       61 tgcaatgggt taaaaacgat gtgggtgaaa ttttcattcg tcagtttgaa agctttgtca
      121 gccgtttttt ggggaatggg catacaagtt gtattttcca ggagtcctgc aaggataatc
      181 tggttgttga aagtaatgga gacatttacg aatgcgacca ttttgtctat ccacagtaca
      241 aaattggaaa cattaataaa tctgaactca aaacgatgaa cagtgtacaa ctgacagcgc
      301 aaaaaaaacg gatttcagcg aaatgtcagc aatgtgcata taaacctatc tgcaatggcg
      361 gttgtcctaa gcatcgtatt actaaagtaa acaatgagac tgtttcttat ttttgcgaag
      421 gttataaaat ccttttttca accatggtac cttatatgaa cgccatggtt gagttagcta
      481 agaacagagt accgctttac cacattatgg atgttgcaaa acaaatggag aataattaat
      541 aacgctctct tttaactcca ttcttagcct ccttcttatg accttaacaa ggcacccgac
      601 ataaattcct gtcgggtgtt tcgtcatcat catcttccct tgcgaaaatc ctggcttacg
      661 tggtatgcgt cagggcatta gcaataccct tgattatcaa tagcataacc ataaccactg
      721 aaaattaaaa atttctatat atttcatatt gttaaatgat gcttaaataa atttcatcag
      781 taaatcattg ccatagataa tcattggatg gcacaatcaa ttttcgctaa tgataaccat
      841 tcccattacg ttttgtatgt tgatagcaaa gtatctctgt ctgctgaagc ccttttggct
      901 gcgtaagaat aataaaactt cagtgttgtt aatcattatt atcctcgcaa tgatccttgg
      961 cgtggttaaa attcaggtct ggttaaacga ctggaataat gattttttca atgcgctaag
     1021 ccagaaagaa accgataagc tttggcagct cgttctttgg ttccctgccc tgctggggat
     1081 ctttgtatta atatccgcaa acaaaacatg gttaattaaa ctactgacta ttcgctggcg
     1141 cgaatggtta acagattact accttaatcg ctggtttgca gataaaaact actacttcac
     1201 gcagatctac ggcgagcata aaaatacaga taaccctgac caacgtatcg ctgaagacat
     1261 tctcttactg atcaacaaaa cgctcagtct ttcatttggc ttcatccagt cacttagcat
     1321 gctgatcacc tttaccgtta ttctttggca aagcgcgggt acgctctcat tcactgttgg
     1381 tggaacagaa tggaatatcc aggggtatat ggtctatacc gtcgtgctca ttgtgatcgg
     1441 tggaacttta tttacccata aggtgggtaa acgtattcgt ccgcttaatg tggaaaaaca
     1501 acgtagcgaa gcgacatttc gaactaatct tgtgcagcat aataagcagg cagaactgat
     1561 tgcgctaagc aatgctgagt ctctacaacg ccaggagttg cgcgacaatt ttcataccat
     1621 taaagagaac tggcatcgtt taatgaatcg tcagcggtgg cttgattact ggcaaaatat
     1681 ttattcgcgc tcgcttagcg ttcttcccta ctttctgtta ttgccgcagt ttattagcgg
     1741 gcagatcaat ctgggcggac tgatgaaatc gcgccaggca tttatgctag tatcgaacaa
     1801 tttaagctgg tttatttata aatatgacga acttgctgaa ctggctgcgg ttatcgatcg
     1861 cttgtatgag ttccatcaac tcactgaata gcgccctacg aataagccta aaaattgcca
     1921 acatgcggta caagtggcta atgcgagtat tcgtacgcct gataataaga tcatattaga
     1981 gaacttgaac tttcatgttt cgccaggcaa atggctatta ctgaaaggct actctggtgc
     2041 gggaaaaacc acactgctta aaacattatc ccactgctgg ccgtggttta aaggtgatat
     2101 ttcttctcct gctgacagtt ggtatgtgtc acaaacaccg ttaatcaaaa gcggcttact
     2161 gaaagagatt atttgtaaag cacttcccct gcccgtagac gataaatcgt tgagcgaagt
     2221 actgcatcag gttggtctgg gaaaattggc tgcgcgtatt catgaccacg atcgctgggg
     2281 agatattctt tccagcggcg aaaaacaacg tatcgccctg gcacgattaa ttttacgacg
     2341 tccgaaatgg atatttcttg acgaaactac ctctcatctt gaggaacaag aggctatacg
     2401 cttactgcgt ttagtgcgtg aaaaactacc cacaagcggc gtcattatgg ttacacatca
     2461 acccggtgtc tggaacctgg ctgatgatat ttgtgacatt agcacggttt tataacaaca
     2521 ataaaagccc tgctgtacgg agatataaat aaatgaagcg agttcttatt cctggcgtca
     2581 ttttatgtgg cgctgatgtg gcgcaggccg tcgatgacaa aaacatgtac atgtattttt
     2641 ttgaagagat gacggtctat gctcctgtcc ctgtacccgt aaacggcaac acgcattaca
     2701 ccagtgaaag catcgagcgt ttaccgaccg ggaatggcaa tatcagcgat ctgctgagaa
     2761 ccaaccctgc ggtacgcatg gattcaacgc aaagtacctc gttgaaccag ggagatattc
     2821 gccctgagaa aatctctatt cacggtgcgt cgccctacca gaatgcctat ttgattgacg
     2881 gtattagtgc aactaataac ctgaacccag cgaatgagtc cgatgccagt agtgcaacca
     2941 atattagcgg gatgtcacag gggtattatc ttgatgtcag cttactggac aatgtgacgc
     3001 tttatgacag ttttgtgccg gttgaatttg gtcgcttcaa tggcggggta attgatgcaa
     3061 agatcaaacg cttcaacgct gatgatagca aggtgaaatt gggttatcgc actacgcgtt
     3121 tggactggtt aacatcgcat atcgatgaga ataacaagag cgcatttaat caaggttctt
     3181 caggaagtac ctatttctct ccagatttta aaaagaactt ttataccttg tcgtttaatc
     3241 aggagctggc tgataacttt ggcgttaccg ccggtttatc gcgccgccag tctgatatca
     3301 cccgcgcgga ttatgtttcg aatgacggca ttgtcgccgg tcgggcacag tataaaaacg
     3361 ttatcgatac tgcattgagc aaatttacct ggtttgccag cgaccgcttt acccacgatt
     3421 taaccttaaa atataccggc tccagccgtg attataatac cagcaccttc ccgcagtctg
     3481 atcgcgaaat gggtaataaa tcctatggtc tggcatggga tatggatacg caactcgcat
     3541 gggccaaact acgtaccacc gttggttggg atcatattag tgattatacc cgtcacgatc
     3601 atgacatctg gtacaccgaa ctttcatgta catatggtga tattacaggg cgttgtaccc
     3661 gtggcggatt aggacacatt tcccaggctg tagataatta caccttcaaa acacgcctgg
     3721 actggcaaaa attcgccgtg ggtgatgttt cgcatcaacc ctacttcggc gcggaataca
     3781 tctattccga tgcatggact gaacgccata accagtctga atcctatgtg attaatgctg
     3841 caggaaagaa aactaaccat accatttacc ataaaggtaa aggcagcctg ggaattgaca
     3901 actacacgct gtatatggcg gatcacatta gctggcggaa tgtgtcgtta atgcccggtg
     3961 tgcgttatga ctatgacaac tatctgtcaa accacaatat ctccccgcgc tttatgacgg
     4021 aatgggatat ttttgctgat caaacctcaa tgattaccgc cggttataac cgttactatg
     4081 gcgggaatat tcttgatatg ggattacgtg atatccgcaa tagctggacg gaatcggtat
     4141 caggtaataa aaccctgacg cgttatcaga atttgaaaac gccttataac gatgaactgg
     4201 caatgggatt gcagcaaaaa atcgataaga acgttattgc acgcgcaagt gaagcgcatg
     4261 atcaaatcag caaaagcagt cgtaccgaca gcgcgactaa aaccaccatt actgaatata
     4321 acaacgatgg caaaaccaaa acgcattcgt ttaacctcag ttttgaactg gccgaacccc
     4381 tgcatatccg ccaggtagat attaacccgc aaattgtctt tagctatatc aagagcaagg
     4441 gcaacttgtc gttaaacaat ggttatgagg agagcaatac cggtgataac caggtggttt
     4501 ataacggtaa tctggtctct tacgatagcg ttccagtggc agattttaat aacccattaa
     4561 agatctcctt aaacatggat ttcacgcatc aaccgagcgg gttagtgtgg gcgaatacgc
     4621 tggcctggca agaagcgcgt aaagctcgca ttatccttgg taagacaaat gcgcaataca
     4681 tcagcgaata ttcagattac aagcagtatg ttgacgaaaa actggatagc agcctgacct
     4741 gggacacccg cttgtcctgg acgccacaat ttctgaaaca acaaaacctg acgatcagtg
     4801 ccgatattct caatgtactg gatagcaaaa ccgctgttga tacaacgaac accggtgtgg
     4861 cgacctacgc cagtggccgt actttctggc ttgatgtcag catgaaattt taatagttcg
     4921 gattttactt aaccgggtaa attcgcccgg ttttcgcatg gagataatta tgagaaacct
     4981 ctgtttctta ctgacgttag tggcaactct gttgctcccc gggcggctga ttgccgccgc
     5041 cttaccgcag gatgaaaagt taattaccgg gcaactggac aatgacttgc gatatatgat
     5101 ttatccgcat gctcatccaa aggatcaggt aaatttatgg ctgcaaattc ataccggttc
     5161 att
//
LOCUS       DAJTSI010000209         5162 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000209 DAJTSI010000000
VERSION     DAJTSI010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.211"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..220
                     /locus_tag="OO327_003776"
     CDS             <1..220
                     /locus_tag="OO327_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6020139.1"
                     /translation="LYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHW
                     GFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(392..721)
                     /locus_tag="OO327_003777"
     CDS             complement(392..721)
                     /locus_tag="OO327_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR6020140.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(726..911)
                     /locus_tag="OO327_003778"
     CDS             complement(726..911)
                     /locus_tag="OO327_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR6020141.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(908..3547)
                     /locus_tag="OO327_003779"
     CDS             complement(908..3547)
                     /locus_tag="OO327_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR6020142.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGLMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3755..4744
                     /gene="ldhA"
                     /locus_tag="OO327_003780"
     CDS             3755..4744
                     /gene="ldhA"
                     /locus_tag="OO327_003780"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6020143.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4855..>5162
                     /gene="hslJ"
                     /locus_tag="OO327_003781"
     CDS             4855..>5162
                     /gene="hslJ"
                     /locus_tag="OO327_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCR6020144.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     "
BASE COUNT         1307 a         1322 c         1203 g         1330 t
ORIGIN      
        1 tttgtaccga atgtttgccg ataaaggttt ggtagtcgcg caatatattc gtaaccgtcg
       61 tctcgatttt tgtgcagatg cgattcgcca tgccgcagat gatgaaaaac tggcaggcat
      121 cggctttcat tggggatttt ctgaccagag tcatttttca acggtattta agcaacgctt
      181 tgggatgacg ccaggcgagt atcgacgtaa attccgctaa ctaattggcg ttgcatgtac
      241 tgcaacgcca attagttaaa acagatattt gactatgcat ttttcagggc gaaaggcaat
      301 ttgacaggag tttttgaata ttcaccttgt ttcaggaaaa tagatcaatc gctttctcct
      361 gtagcgttat cccgatattg cggttttgct attaaaactt tcgcacccat ttaccgttta
      421 tcccttgtac atattccccc ggtttggccc gagctacgag cttttgcccg gcgagtttcg
      481 cgatatcatc tacagacaca ttattttgct ttgcccgttg ttggtaactc gctttacgtt
      541 cggcattaat ttcggttact aatttctctg tttcagcatc cgttttcagc gcaaccagat
      601 aaccataaaa tgtttcacct acccgcccct gagttctggc ttcatccaga gtcaatgcca
      661 ttacattgct gcttaccagc ccaacaagaa acgcacaaag aagtaatgtt ttcttcatca
      721 tcgcctcaga aaagatcgct acgagtttca agcagctctt cgacatcttt gtctgccttg
      781 atgatgatct catgctcaat tttaacgttc atattgatag tgatcggttc cttaggtgca
      841 gcgacttcaa tgcgaggcgt acagccaacc agcataaaag atgacgtcaa cgcagccagt
      901 aaaattttca ttgtttttcc tcacactctt ttccttgcgg acagtcattt cccggcagac
      961 gtgcgttctg ctccaaccat gcctggagat tatcgccaaa gcgtaaactg cgccacagcg
     1021 taaaaatatt ctcttcatga tggtaattaa ggtttaccgt accgctttta ccatcaacgc
     1081 gactggtacc tgtaatgttg gcctgcatgg ttaataaacc gagattatct aagttaattt
     1141 ttgtcgatga ccggctaatt tccatatagc gcaaccagtt aattgctgaa cccgcagtca
     1201 tattgtcttt caccaccgca tctgcggtat ctttatccag ccgcaaggtc attagcccgc
     1261 tatttgccag ccaaccatct ttcactatcc atttttcgtt gtttagccat aacggcaatg
     1321 caccactaaa tgccccggac atggcgaatt gtttcggatt gacggcgcta accagttcgc
     1381 tggatgagag attattcagc cgcaacagcg ccgggtcatg ttgcggcata cgtaattgtt
     1441 tcatcagcac gttaccgcct aacacatcga cgctaacatc agtcaacagc aagggttctt
     1501 cttcagtcca cggataacgc ccttgcaaat cagccgtaat atttttcgct gtcaccagat
     1561 taatcacttc ggcaattcgc aacgtaacgg ggccgcgagt ccccagatgc caggctccat
     1621 cggcaaaacg gaaaggcagg acaaaatcga caccgttaac ctgattatct ggcatccagg
     1681 cactaccgcc tttcaacacg ccgtgccctc ccgcgcggaa tccttgttca ggcgcagcag
     1741 aaaatgcaac ctgagcatac agttcaccat cgcgtaagtt catcttccag tcgggtggca
     1801 ccagcggctg gaatacagtc agtgattgtt ttggccacca ggcgttaccg cgcagacgaa
     1861 taccgtccca gcgaccattt acccgaaccg ggccaatctc accagcatgt aaatcgcctt
     1921 taaagaggaa ataggtcgga ttgcgcccat cgacgctaaa ttttaaggtt gatggcggta
     1981 acacactgcc gccagtgaac agcgtttgcc cggcgtccag tgacagcgcg ccgctgaagg
     2041 agggatgctg tgcgtcacgt acccagacga cgggcttgtc gagaattaat cgcggctttt
     2101 ctaccgtcat cgtaccgtat tgtaactggt cgaaaccggt ggagagatcg gtcagcgtaa
     2161 tcgtgctgtc atgccactca ccttttcctg cgacatccca ggtggcattc atcggtgtaa
     2221 aactcccttt tccccagtag cgccactgcc agcggccagc gtcagggaga aaatcattcg
     2281 ccagcccatc catatgcagc acgaaatcgc ccagttcatt ttcatgcgcc tgcaaaatgg
     2341 cctgcaaacg tccgtcaaca ccacgttggg tgacttttac acccgctaaa ggccagcgga
     2401 tttcatcgat gtccagcgaa tcgatgactc ttccctttga acgaagtaac gcgcctggct
     2461 caaaggtcag cgttgggtca gacagacttc cacttagctg cgcaggtaaa cgggcatata
     2521 aaatgaggtc cgcctgtttc gcttcaccgg tcagctgcag aggcagttga ctgttatcca
     2581 tgcttaattt tcctgggcca aaattaagca ccgcgttgcc cttacccgct tgcccctggg
     2641 tcagcacatt cagtcgtccg ctgaccagag cgttctcaag ccctgcctgc cagttgtcga
     2701 ctttgacacc cagtcggcca ctcaaaggaa aaccagcata cagccagctc cagcgaccat
     2761 cgcttacggt caattgttga cgagtaattt gccacggcaa atcgagcaac ggatcgccgt
     2821 tatcccgtgc cagcacgatc aattgcccgc tattttcctg ccagtccagc tcggcatcca
     2881 ccagtgacgg ttcctgcggc aagtttaacg tcgcagtagc atgcccactt acaggaagtc
     2941 catccggcac gagcggcata gtaaattccc ccaccagttt aaccggcggc tgattttcaa
     3001 acgcgacgac atccagttcg ctgactgtaa gttgttgccc tttcagctgg ccttgaaatt
     3061 taactttttc gccctgataa cgcagttgct ggatatcaga ggttaatgcg agagagagtt
     3121 ttccctgcca ttcctgccag ggagaaaaaa tcagtttatc gatattgatc caggtgttag
     3181 gcagcatgga ctgccactgc gcgagggttt ttggagcagc tggcgattgc tccgtctgcg
     3241 gcaatttcgc cagacaagca gaatcaagtt ctaccgtgcc gacgttcaat aaccagcggc
     3301 tgggatgtga aaggctggcg ttggtgatat gcgcaagctg acaatctccc accagataac
     3361 ggagatcggg aatgattaaa cctttacgcg taatgcgtgg gctttcatct aatgcaatac
     3421 gtgtcccgag cggtagccag atgtccgcca gcgtgggaac ccacagcccg agcgtcatca
     3481 gcagcgtcaa cggcacaaga ataatcagta ataacagcgc gagaacggct ttatatttac
     3541 ccagcatggg tagttaatat cctgatttag cgaaaaatta agcattcaat acgggtattg
     3601 tggcatgttt aaccgttcag ttgaaggttg cgcctacact aagcatagtt gttgatgaat
     3661 ttttcaatat cgccatggct ttcaattata tttgaaattt tgtaaaatat ttttagtagc
     3721 ttaaatgtga ttcaacatca ctggagaaag tcttatgaaa ctcgccgttt atagcacaaa
     3781 acagtacgac aagaagtacc tgcaacaggt gaacgagtcc tttggctttg agctggaatt
     3841 ttttgacttt ctgctgacgg aaaaaaccgc taaaactgcc aatggctgcg aagcggtatg
     3901 tattttcgta aacgatgacg gcagccaccc ggtgctggaa gagctgaaaa agcacggcgt
     3961 taaatatatc gccctgcgct gtgccggttt caataacgtc gaccttgatg cggcaaaaga
     4021 actggggctg aaagtagtcc gtgttccagc ctatgatcca gaggccgttg ctgaacacgc
     4081 catcggtatg atgatgacgc tgaaccgccg tattcaccgc gcgtatcagc gtacccgtga
     4141 cgctaacttc tctctggaag gtctgaccgg ctttactatg tatggcaaaa cggcaggcgt
     4201 tatcggtacc ggtaaaatcg gtgtggcgat gctgcgcatt ctgaaaggtt ttggtatgcg
     4261 tctgctggcg ttcgatccgt atccaagtgc agcggcgctg gaactcggtg tggagtatgt
     4321 cgatctgcca accctgttct ctgaatcaga cgttatctct ctgcactgcc cgctgacacc
     4381 ggaaaactac catctgttga acgaagccgc cttcgatcag atgaaaaatg gcgtgatgat
     4441 cgtcaatacc agtcgcggtg cattgattga ttctcaggcg gcaattgaag cgctgaaaaa
     4501 tcagaaaatt ggttcgttgg gtatggacgt ctatgagaac gaacgcgatc tgttctttga
     4561 agataaatcc aacgacgtga tccaggatga cgtattccgt cgcctgtctg cctgccacaa
     4621 cgtgctgttt accgggcacc aggcattcct gacagcagaa gctctgacca gtatttctca
     4681 gactacgctg caaaacttaa gcaatctgga aaaaggtgaa acctgcccga acgaactggt
     4741 ttaatcttgc agctcccctg caatccaggg gagctgattc agataatccc caatgacctt
     4801 tcattctcta ttcttaaaat agtcctgagt cagaaactgt aattgagaac cacaatgaag
     4861 aaagtagccg cgtttgttgc gctaagcctg ctgatggcgg gatgtgtaag taatgacaaa
     4921 attgctgtta cgccagaaca gctacagcat catcgctttg tgctggaaag cgtaaacggt
     4981 aagcccgtga ccagcgataa aaatccgcca gaaatcagct ttggtgaaaa aatgatgatt
     5041 tccggcagca tgtgtaaccg ctttagcggt gaaggcaaac tgtctaatgg tgaactgaca
     5101 gccaaagggc tggcaatgac ccgtatgatg tgcgctaacc cgcagcttaa tgaactcgat
     5161 aa
//
LOCUS       DAJTSI010000210         5151 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000210 DAJTSI010000000
VERSION     DAJTSI010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.212"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            190..447
                     /locus_tag="OO327_003782"
                     /pseudo
     CDS             190..447
                     /locus_tag="OO327_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(497..1447)
                     /gene="uspE"
                     /locus_tag="OO327_003783"
     CDS             complement(497..1447)
                     /gene="uspE"
                     /locus_tag="OO327_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR6020145.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="OO327_003784"
     CDS             complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="OO327_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR6020146.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="OO327_003785"
     CDS             complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="OO327_003785"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR6020147.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3072..3215)
                     /locus_tag="OO327_003786"
                     /pseudo
     CDS             complement(3072..3215)
                     /locus_tag="OO327_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3605..4420)
                     /locus_tag="OO327_003787"
     CDS             complement(3605..4420)
                     /locus_tag="OO327_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6020148.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4517..>5151
                     /locus_tag="OO327_003788"
     CDS             4517..>5151
                     /locus_tag="OO327_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6020149.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTL"
BASE COUNT         1374 a         1175 c         1226 g         1376 t
ORIGIN      
        1 gctattttac cgcgacaatt cattcagaac atcaatagtc agggaaggaa gtagcaacat
       61 tagctaacta atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag
      121 acaacggtga aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag
      181 gattacatca tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt
      241 ttgttggcgg ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt
      301 atagaacagt acaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca
      361 tcgatgattt ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt
      421 tatttccttg gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc
      481 agttattgaa gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact
      541 ggtcaggttt aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt
      601 tgccgaggaa tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg
      661 cctgtaaatg ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg
      721 tcatgttttc attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc
      781 cacgaatggc atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa
      841 ttggcgttac cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca
      901 gttcgatcgt ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg
      961 cgagattcac cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc
     1021 acaccgggct tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt
     1081 ccagacggtc atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt
     1141 cctgaatgat ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc
     1201 cagcattgag ataatatttt gcctgctcgt ggatccaggc tgtacgctgg ctgatgacgc
     1261 cctgacgcat agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt
     1321 catagatcgg caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc
     1381 gccgcaatgc tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca
     1441 tagccataca gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc
     1501 catatctggt gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt
     1561 aatgatatga cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatgg
     1621 ccagcaagct gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc
     1681 agcataccgc ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc
     1741 agatagttac caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg
     1801 cgttgggcaa aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt
     1861 ttcttcgaca acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc
     1921 atcatctgct gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga
     1981 atttcacata ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg
     2041 atggcgtcaa atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct
     2101 tgctcagtga ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt
     2161 tcatcaccag ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata
     2221 ttatcaagct gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg
     2281 ctgcaatcct ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt
     2341 tccgggatca taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta
     2401 gggagcaagt aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata
     2461 tatgtctatc tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa
     2521 caagctggca caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc
     2581 actcttttcg ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc
     2641 gatggcaagg tacgacgatg ctgatgggat tcgatccgtt tgccgcacca acggcacgcg
     2701 ccgcgccagg gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg
     2761 ggatagtgcg tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg
     2821 gaagcgtatc aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac
     2881 cacctgggtt ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca
     2941 gctgcaccat gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat
     3001 cgcaaatcac ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca
     3061 tccgttctct cttaagacaa acgtgggtaa ataccctgac ctatcggcag accgacaaga
     3121 taccacgcca gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact
     3181 aacgaatagt aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga
     3241 ttttttaatt tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga
     3301 taattccagt cagtgaaatt ttaccgtagg ctatctgtta tttgattctg tggcgtgata
     3361 gccgccagac accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt
     3421 tacagagcta aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt
     3481 acacacagca gtgcatcctc gtcttaaatc cggcctgggg tcctggtgac cggtgacagt
     3541 gttataacgg ttttacccat ccgttgccaa ttttcttcag tgacagattt cacgaaaata
     3601 taagttaaat catttgtctt gtgattgcat aattatgcat cgttaatcga cgagtaaatt
     3661 taccgggcga gtagcccgtt cgacgcttaa acatattgcc gaacgattca gctgaggcat
     3721 agcccagacg atgggcaatt ttggtcaacg aatatccatc atcaagaagc gtcactgcag
     3781 aaataacgtg catttgctga acccagtggc gaaatgttaa tcccgtctct ttaacaaata
     3841 agcgcatgag tgtacggcta ctgacgttaa ttttatccgc tagattatcc agtgttggca
     3901 gatacccttg gttgtttagc aaattctcac aaagctgctg tagccgctca tcatttggcc
     3961 aagggagttc aagtgacatt ttcaccccca tttttatttc ctgaagtatc agtgtctcga
     4021 gggctgaaaa acgtaactgt tgtttatctg tggtactttg cgacggtgga agttggttaa
     4081 ttgcaattag taattccctt aacaggggtg aaatacccgt catgaagcaa actgttccca
     4141 gcgtttcact ttccgcttct gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa
     4201 caaacttatg cggcatttgt gggggcagcc aaagcgcgct gctggcatgg actagccaga
     4261 cgctttctcc agcccataca cgaacgactc cttcagccgg gtaaagcaac tgggcataga
     4321 gatgtttatg ccagggaact gtacctccat tcagatgttc cgaatccagt ccgataatgt
     4381 gatgccctgg tgatgctttg ggttgcagtg taattgtcat ttacagtagg tattttgtca
     4441 gaaatcatca aataagccat gttaaatttt aaatatgacg tatgcaagtt tccggatata
     4501 tacggtgtaa agagtgatga taatttctaa aaaacagctt attggggtgg tagcgatcgg
     4561 tattcttttg actggcgttg tcttttttat ctggagggta agcaaaggaa gatttataca
     4621 gacgacggat gatgcttaca ttggtggtaa tatcaccacg gtggcaagta aagtttctgg
     4681 ttatatttct gctatagagg tgcgcgataa tcagagcgta aaaaaaggcg atattatcct
     4741 gcgtctcgat gatcgggatt atcgtgcaaa tgtcgccagg cttgaggcaa aaataaaaag
     4801 tagcaaagcc aatctcgaag gcattcaagc cactattacg atgcagcagt cgataattca
     4861 aagtgccagt gaaacatggc aggcagtaaa acatgaggag caaaagcgcc tgcgagatac
     4921 tgaacgttat gaaaaactcg cacaatcagc ggcaatatcg caacaaatta ttgataacgc
     4981 caggtttgac taccagcagg tcgcagcaaa ggagcgcaaa gccgcgaacg atttcctggt
     5041 ggagaagcaa cggctggcag ttttatctgc tcaggaagaa aacgtacgtg cgagtatcga
     5101 agaagttcag gccgctttga ctcaggcact actggatctg gaatataccc t
//
LOCUS       DAJTSI010000211         5117 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000211 DAJTSI010000000
VERSION     DAJTSI010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5117
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.213"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1397
                     /gene="selB"
                     /locus_tag="OO327_003789"
     CDS             <1..1397
                     /gene="selB"
                     /locus_tag="OO327_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR6020150.1"
                     /translation="GRGIDALREHLLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTG
                     TALSGEVKVGDSLWLTGVNKPMRVRALHAQNQPTETANAGQRIALDIAGDAEKEQINR
                     GDWLLADVPPEPFTRVIVELQTHAPLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFD
                     TPLWLADNDRLVLRDISARNTLAGARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEA
                     LAVHLQRGAVNLPDFAWARQLNGEGMRELLQQPGYIQAGYSLLNAPVAARWQRKILDT
                     LATYHEQHRDEPGPGRERLRRMALPMEDEALVLLLIEKMRESGDILSHHGWLHLPDHK
                     AGFSEEQQAIWQKAEPLFGDEPWWVRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKD
                     RYYRNDRIVEFANMIRDLDQECGSTCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRG
                     NDHLLRDALLFPEK"
     gene            1587..2738
                     /gene="yiaY"
                     /locus_tag="OO327_003790"
     CDS             1587..2738
                     /gene="yiaY"
                     /locus_tag="OO327_003790"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCR6020151.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            2903..4441
                     /gene="aldB"
                     /locus_tag="OO327_003791"
     CDS             2903..4441
                     /gene="aldB"
                     /locus_tag="OO327_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCR6020152.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(4660..4803)
                     /locus_tag="OO327_003792"
     CDS             complement(4660..4803)
                     /locus_tag="OO327_003792"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020153.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            4986..>5117
                     /locus_tag="OO327_003793"
     CDS             4986..>5117
                     /locus_tag="OO327_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR6020154.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     "
BASE COUNT         1267 a         1240 c         1367 g         1243 t
ORIGIN      
        1 aaggtcgggg aattgatgcc ctgcgcgagc atctgcttca gttgccggaa cgcgagcacg
       61 ccagccaaca tagtttccgc ctcgcgattg accgcgcatt taccgtaaaa ggtgccgggc
      121 tggtcgtcac cggtacggcg ttaagcgggg aagtgaaggt aggcgattca ctctggctga
      181 ctggtgtaaa taaaccgatg cgtgtacgtg cgctgcatgc gcaaaaccag ccaacagaaa
      241 ccgccaatgc cgggcagcgt atcgcgcttg acatcgcggg tgatgcggaa aaagagcaga
      301 ttaaccgtgg cgactggctg cttgccgatg tgccgccaga gccgttcaca cgggtgattg
      361 tcgagcttca aacccatgca ccgctgaccc agtggcagcc gctgcatatt caccacgccg
      421 ccagccacgt cacgggacgc gtttcactgc tggaagataa ccttgctgaa ctggtcttcg
      481 acaccccgtt atggctggca gataacgacc gcctggtatt gcgcgatatc tctgcccgca
      541 acacgctggc cggagcgcgc gtcgtgatgc ttaacccgcc gcgtcgcggt aaacgtaaac
      601 cagagtatct gcaatggctg gcttccctcg ccagggcaca gaatgatagc gaagcgttag
      661 ccgttcacct gcaacgcggc gcggttaatc ttcctgattt cgcttgggcg cgccagctca
      721 acggcgaagg gatgcgcgaa ctgctgcaac agcctggcta tattcaggct ggttatagct
      781 tgttgaatgc gccggttgcc gctcgctggc aacggaaaat tctcgacaca ttagcgactt
      841 atcatgagca acatcgcgat gaacctgggc ctgggcgcga acgtctgcga cgtatggcgt
      901 tgccaatgga agatgaagcg ctagtactgt tgctgattga aaagatgcgc gaaagcggcg
      961 acatcctcag tcatcacggt tggctgcatc tgccggatca caaagcgggc ttcagcgaag
     1021 agcagcaggc catctggcaa aaagcagagc cgctgtttgg tgacgagccg tggtgggtgc
     1081 gtgatctggc aaaagagacg ggtaccgacg agcaggcaat gcgcctgacg ctacgccagg
     1141 cggcgcagca aggaataatt accgcgatcg tcaaagatcg ttattaccgt aacgatcgga
     1201 ttgtcgaatt tgccaatatg atccgcgatc tcgatcagga gtgtggttca acctgcgcgg
     1261 cggatttccg cgatcgctta ggcgtaggcc gaaagctggc aattcagatt ctggaatatt
     1321 ttgaccgcat tggctttacg cgtcgtcgtg gaaatgatca tttattgcgc gacgcattat
     1381 tatttccgga aaaataagga aatgattaat taatttttaa aataaattaa tacaaatttc
     1441 ttatgatttt aaataaagca cattgtttaa tgaatacaat gtgcttttta ttagattaat
     1501 tttgataatt aataataatg atcatgtttt cgaatgaaaa tcctcagtaa gctgcccacc
     1561 cttttttaca ctttcaggag tgtgttatgg catcttcaac tttctttatt ccttctgtga
     1621 atgtcatcgg cgctgattca ttgactgatg caatgaatat gatggcagat tatggattta
     1681 cccgtacctt aattgtcact gacaatatgt taacgaaatt aggtatggcg ggtgatgtgc
     1741 aaaaagcact ggaagaacgc aatattttta gcgttattta tgatggcacc caacctaacc
     1801 ccaccacgga aaacgtcgcc gcaggtttga aattacttaa agaaaataat tgcgatagcg
     1861 tgatctcctt aggcggtggt tctccacacg actgcgcaaa aggtattgcg ctggtggcag
     1921 ccaatggcgg cgatattcgc gattacgaag gcgttgaccg ctctgcaaaa ccgcagctgc
     1981 cgatgatcgc catcaatacc acggcgggta cggcctctga aatgacccgt ttctgcatca
     2041 tcactgacga agcgcgtcat aacaaaatgg cgattgttga taagcacgtg actccgctgc
     2101 tttctgtcaa tgactcctct ctgatgattg gtatgccgaa gtcactgacc gccgcaacgg
     2161 gtatggatgc cttaacgcac gctatcgaag cgtatgtttc tattgccgcc acgccgatca
     2221 ctgacgcttg tgcactgaaa gccgtgacca tgattgccga aaacctgccg ttagccgttg
     2281 aagatggcag taatgcgaaa gcgcgtgaag caatggctta tgcccagttc cttgccggta
     2341 tggcgttcaa taatgcttct ctgggttatg ttcatgcgat ggcgcaccag ctgggcggtt
     2401 tctacaacct gccacacggt gtatgtaacg ccgttctgct gccgcatgtt caggtattca
     2461 acagcaaagt cgccgctgca cgtctgcgtg actgtgccgc tgcaatgggc gtgaacgtga
     2521 caggtaaaaa cgacgcggaa ggtgctgaag cctgcattaa cgccatccgt gaactggcga
     2581 agaaagtgga tatcccggca ggcctacgcg acctgaacgt gaaagaagaa gatttcgcgg
     2641 ttctggcgac taatgccctg aaagatgcct gtggctttac taacccgatc caggcaactc
     2701 acgaagaaat tgtggtgatt tatcgcgccg caatgtaatc atcattccaa caacggctgg
     2761 caaattgtca gccgcttttt caactatctc tgtaatcctt gcccgtaaat tcgtgatagc
     2821 tgtcgtaaag ctgttaccga ctggcgaaga tttcgccagt cacgtctacc cttgttatac
     2881 ctcacaccgc aaggagacga tcatgaccaa taatccccct tcagcacaga ttaagcccgg
     2941 cgagtatggt ttccccatca agttaaaagc ccgctacgac aactttattg gcggtgaatg
     3001 ggtagcccct gccgatggtg agtattacca gaatctgacg ccggtgaccg ggcagctgct
     3061 gtgcgaagtg gcgtcttcgg gcaaacgaga catcgatctg gcgctggatg ctgcgcacaa
     3121 agtgaaagat aaatgggcgc acacctcggt gcaggatcgc gcggcgattc tgtttaagat
     3181 tgccgatcga atggaacaaa acctcgagct gttagcgaca gctgaaacct gggataacgg
     3241 caaacccatt cgcgaaacca gtgctgcgga tgtaccgctg gcgattgacc atttccgcta
     3301 tttcgcctcg tgtattcggg cgcaggaagg tgggatcagt gaagttgata gcgaaaccgt
     3361 ggcctatcat ttccatgaac cgttaggcgt ggtggggcag attatcccgt ggaacttccc
     3421 gctgctgatg gcgagctgga aaatggctcc cgcgctggcg gcgggcaact gtgtggtgct
     3481 gaaacccgca cgtcttaccc cgctttctgt actgctgcta atggaaattg tcggtgattt
     3541 actgccgccg ggcgtggtga acgtggtcaa tggcgcaggt ggggaaattg gcgaatatct
     3601 ggcgacctcg aaacgcatcg ccaaagtggc gtttaccggc tcaacggaag tgggccaaca
     3661 aattatgcaa tacgcaacgc aaaacattat tccggtgacg ctggagttgg gcggtaagtc
     3721 gccaaatatc ttctttgctg atgtgatgga tgaagaagat gcctttttcg ataaagcgct
     3781 ggaaggcttt gcactgtttg cctttaacca gggcgaagtt tgcacctgtc cgagtcgtgc
     3841 cttagtgcag gagtcaatct acgaacgctt tatggaacgc gccatccgcc gtgtcgaaag
     3901 cattcgcagc ggtaacccgc tcgacagcgt gacgcaaatg ggcgcgcagg tttctcacgg
     3961 gcaactggaa accatcctca actacattga tatcggtaaa aaagagggcg ctgacgtgct
     4021 cacaggcggg cggcgcaagc tgctggaagg tgaactgaaa gacggctact acctcgaacc
     4081 gacgattctg tttggtcaga acaatatgcg ggtgttccag gaggagattt ttggcccggt
     4141 gctggcggtg accaccttca aaacgatgga agaagcgctg gagctggcga acgatacgca
     4201 atatggcctg ggcgcgggcg tctggagccg caacggtaat ctggcctata agatggggcg
     4261 cggcatacag gctgggcgcg tgtggaccaa ctgttatcac gcttacccgg cacatgctgc
     4321 gtttggtggc tacaaacaat caggtatcgg tcgcgaaacc cacaagatga tgctggagca
     4381 ttaccagcaa accaagtgcc tgctggtgag ctactcggat aaaccgttgg ggctgttctg
     4441 atataagaag ctggtcgcat tgggtattca ttgcctgatg cgacgcttac gcgtcttatc
     4501 atgcctacgg gaacctgacc gtaggccgga taaggcgtac acgccgcatc cggcataaac
     4561 aaagcgcacg ttgttacaat ttacgtttta aacctacgtg cgcaataacc cagactacgc
     4621 caacagaagt gcgtttctgg ggcgtgtttt gtcaggagtt taatctcgcg tttcgatggt
     4681 gagcgggcga accaggccgt aaatcaccat tggtgatccg aggccaatca gcaataaacg
     4741 gtagataatt gaaggtttaa tacgcttaat ctgtactgtg gatgttttac tggagttatt
     4801 cattagtttg gccaatagtt ccactatttc aatatgataa acctcgtcct gttgaaaaac
     4861 cagacagcac atttcagaaa gtatataaaa aacaatatgt cgttatataa ataaggaaat
     4921 tgcactgcat catggcattt cataatatgc gttaattgtt cttttttata ttctttgagg
     4981 gaaatatgtt cctggactat tttgcactgg gagtgcttat ttttgtattt ctggtgatat
     5041 tctatgggat tattatttta catgatattc cctacctgat tgctaaaaaa cgtaatcatc
     5101 ctcatgccga cgccatt
//
LOCUS       DAJTSI010000212         5024 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000212 DAJTSI010000000
VERSION     DAJTSI010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.214"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            48..122
                     /gene="tnaC"
                     /locus_tag="OO327_003794"
     CDS             48..122
                     /gene="tnaC"
                     /locus_tag="OO327_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCR6020155.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            343..1758
                     /gene="tnaA"
                     /locus_tag="OO327_003795"
     CDS             343..1758
                     /gene="tnaA"
                     /locus_tag="OO327_003795"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCR6020156.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1849..3096
                     /gene="tnaB"
                     /locus_tag="OO327_003796"
     CDS             1849..3096
                     /gene="tnaB"
                     /locus_tag="OO327_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCR6020157.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3228..4403
                     /gene="mdtL"
                     /locus_tag="OO327_003797"
     CDS             3228..4403
                     /gene="mdtL"
                     /locus_tag="OO327_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR6020158.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4378..>5024
                     /gene="yidZ"
                     /locus_tag="OO327_003798"
     CDS             4378..>5024
                     /gene="yidZ"
                     /locus_tag="OO327_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR6020159.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
BASE COUNT         1144 a         1208 c         1256 g         1416 t
ORIGIN      
        1 tctgagtgta ataatgtagc ggcgtgtctt tcgaggataa gtgcattatg aatatcttac
       61 atgtatgtgt gacctcaaaa tggttcaata tcgacaataa gattgtcgat caccgccctt
      121 aatttaccct tctgtagcca tcaccagagc caaaccgatt agattcaatg tgatcgatta
      181 gtttgctata tcttaatttt gccttttgca aaggccatct ctcgtttatt tacttatttt
      241 agtaaatgat ggtgcttgta tatatacatg gtgaattaat ggatattgca gatgtaatat
      301 tcacagggat cactgtaatt aaaataaatg aaggattatg taatggaaaa ctttaaacat
      361 ctccctgaac cgttccgcat tcgtgttatt gagccagtaa aacgtactac tcgcgcttat
      421 cgtgaagaag caattattaa atccggtatg aacccgttcc tgctggatag cgaagatgtg
      481 tttatcgatt tactgaccga cagcggcacc ggggcggtaa cccaaagtat gcaggcagcg
      541 atgatgcgcg gcgacgaagc ctacagcggc agccgcagct actatgcgtt agccgagtca
      601 gtgaaaaata tctttggtta tcaatatact attccgactc accagggccg tggcgcagag
      661 caaatctata ttccggtact gattaaaaaa cgcgagcagg aaaaaggcct ggatcgcagc
      721 aaaatggtgg cattctctaa ctatttcttt gataccacgc agggccatag ccagattaac
      781 ggctgtaccg tgcgtaacgt ctatatcaaa gaagccttcg atacgggcgt gcgttacgac
      841 tttaaaggca actttgacct cgaaggatta gaacgcggta ttgaagaagt tggcccgaat
      901 aacgtgccgt atatcgttgc aaccatcacc agtaactctg caggtggtca gccggtttca
      961 ctggcaaact taaaagcgat gtacagcatc gcgaagaaat acgatattcc ggtggtaatg
     1021 gactccgcac gctttgctga aaacgcctat ttcatcaagc agcgtgaagc agaatacaaa
     1081 gactggacca tcgagcagat cacccgcgaa acctacaaat atgccgatat gctggcgatg
     1141 tccgccaaga aagatgcgat ggtgccgatg ggcggcttgc tgtgcatgaa agacgacagc
     1201 ttctttgatg tgtacaccga gtgcagaacc ctttgcgtgg tgcaggaagg cttcccgaca
     1261 tatggcggct tggaaggcgg cgcgatggag cgtctggcgg taggtctgta tgacggcatg
     1321 aatctcgact ggctggctta tcgtatcgcg caggtacagt atctggtcga tggtctggaa
     1381 gagattggcg ttgtctgcca gcaggcgggc ggtcacgcgg cattcgttga tgccggtaaa
     1441 ctgctgccgc atatcccggc agaccagttc ccggcacagg cgctggcgtg cgagctgtat
     1501 aaagtcgccg gtatccgtgc ggtagaaatt ggctctttcc tgttaggccg cgatccgaaa
     1561 accggtaaac aactgccatg cccggctgaa ctgctgcgtt taaccattcc gcgcgcaaca
     1621 tatactcaaa cacatatgga cttcattatt gaagccttta aacatgtaaa agagaacgcg
     1681 gcgaatatta aaggattaac ctttacctac gaaccaaaag tattgcgtca cttcaccgca
     1741 aaactgaaag aagtttaatt aatactacag agtggctata aggatgttag ccactctctt
     1801 accctacatc ctcaataaca aaaatagcct tcctctaaag gtggcatcat gactgatcaa
     1861 gctgaaaaaa agcactctgc attttggggt gttatggtta tagcaggtac agtaattggt
     1921 ggaggtatgt ttgctttacc tgttgatctt gccggtgcct ggtttttctg gggtgccttt
     1981 atccttatca ttgcctggtt ttcaatgctt cattccgggt tattgttatt agaagcaaat
     2041 ttaaattatc ctgtcggctc cagttttaac accatcacca aagatttaat aggtaacacc
     2101 tggaacatta tcagcggtat taccgttgcc ttcgttctct atatcctcac ttatgcctat
     2161 atctctgcta atggtgcgat cattagtgaa acgatatcaa tgaatttggg ctatcacgct
     2221 aatccacgta ttgtcgggat ctgcacagcc attttcgttg ccagcgtatt gtggataagt
     2281 tcgttagccg ccagtcgcat tacctcattg ttcctcgggc tgaagattat ctcctttgtg
     2341 atcgtgtttg gttctttctt cttccaggtc gattactcca tcctgcgcga tgccaccagc
     2401 accactgcgg gaacgtctta cttcccgtat atctttatgg ctttgccggt gtgtctggcg
     2461 tcatttggtt tccacggcaa tattcccagc ctgattattt gctatggcaa acgcaaagat
     2521 aagttaatca aaagcgtggt atttggttcg ctgctggcgc tggtgattta tctcttctgg
     2581 ctctattgca cgatggggaa tattccgcgc gaaagcttta aggcgataat ctcctcaggc
     2641 ggcaacattg attcgctggt gaaatcgttc ctcggcacca aacagcacgg cattatcgag
     2701 ttttgcctgc tggtgttctc taacttagct gttgccagtt cgttctttgg tgtcacgctg
     2761 gggttgttcg attatctggc ggacctgttt aagattgata actcccacgg cgggcgtttc
     2821 aaaaccgtgc tgttaacctt cctgccacct gcgttgttgt atctgatctt cccgaacggc
     2881 tttatttacg ggatcggcgg tgccgggctg tgcgccacca tctgggcggt cattattccc
     2941 gcagtgcttg caatcaaagc tcgcaagaag tttcccaatc agatgttcac ggtctggggc
     3001 ggcaatctta ttccggcgat tgtcattctc tttggtataa ccgtgatttt gtgctggttc
     3061 ggcaacgtct ttaacgtgtt acctaaattt ggctaaatcc ttcaagaagc cagccattcg
     3121 ctggcttctt gcctctcagg aaatcactta tgtccaaatg gcaactcgcc ttatcctcct
     3181 tcaccacgta tgctttgcgt caccttacta tcaggacgct ttagcccatg tcccgctttt
     3241 tgatttgtag ttttgccctg gttttacttt atcccgccgg gattgatatg tacctcgttg
     3301 gtttaccgcg catcgccgcc gatctcaatg ccagcgaagc gcagttgcat attgcgttct
     3361 ccgtatatct ggcggggatg gcagctgcga tgctatttgc cggtaaagtg gccgatcgtt
     3421 cagggagaaa gccggtcgcc atacccggcg cggcgctatt tattattgcc tcggtgttct
     3481 gttcactggc tgaaaccagc acgttatttc ttgcaggccg atttctacag gggttgggcg
     3541 caggctgttg ttacgtggtg gcgttcgcca ttttgcgcga cacgctggat gatcgacgtc
     3601 gggctaaagt gctgtcatta ctcaacggta ttacctgcat cattccggtg ttagcgccag
     3661 tgctcggaca tctgattatg cttaaattcc cgtggcagag tctgttctgg gcgatggcaa
     3721 tgatgggcat cgcggtactg atgttgtctt tgtttatttt aaaagaaacg cgcccagcgt
     3781 cccccgctgc ttcggacaaa ccacgagaaa atagcgagtc gctgcttaat cgttttttcc
     3841 tcagccgtgt tgttatcacc accctcagcg tttcggtgat cctcactttc gtcaatacat
     3901 cgccggtatt gctgatggaa atcatggggt ttgagcgcgg agaatacgcc accattatgg
     3961 cgctgaccgc tggcgtcagc atgacctttt cattctcgac gccatttgcg ctgggaattt
     4021 ttaagccacg tacgttgatg atcacctcgc aggtgttatt ccttgcggcg gggatcaccc
     4081 ttgccgtttc accttcccat gcggtttctc tgtttggtat cacgctgatt tgcgccggtt
     4141 tctcggtagg ttttggcgta gcgatgagtc aggcgttagg accattttca ttacgcgcgg
     4201 gcgtagccag ctcgacctta ggtattgcgc aggtttgcgg ttcgtcactg tggatttggc
     4261 tggccgcggt ggttggtatc ggcgcatgga atatgctgat cgggattctg attgcctgta
     4321 gcatagtgag cctgttgctg attatgttcg tcgcgcctgg acgccccgtt gccgctcatg
     4381 aagaaatcca tcaccacgct tgatctcaat ctgctgctct gtctgcaact gctgatgcag
     4441 gagcgcagcg tgaccaaagc ggcgaagcgg atgaacgtga ccccttcagc ggtgagtaag
     4501 tcgctggcaa agttaagagc gtggtttgac gacccgctct ttgtgaactc accgctgggg
     4561 ctgtcgccca cacagctaat ggtcagcatg gagcaaaatc tggcggaatg gatgcaaatg
     4621 agcaatctgc tgctggataa accgcaccac cagacaccgc gcggcctgaa gtttgagttg
     4681 gcggcggaat cgccgctgat gatgatcatg cttaatgcgc tgtcgaaacg gatctaccaa
     4741 cgttacccgc aggcgaccat caaactacgt aactgggatt acgattcctt agatgccatt
     4801 actcgtggtg aagtggatat tggtttttcc ggtcgcgaaa gccatcctcg ctcgcgggag
     4861 ctgttaagct cgctaccgtt agccattgat tatgaagtgc tgtttagtga tgtaccctgc
     4921 gtctggttac gcaaagatca tccggcactg catgaaacgt ggaatctgga caccttttta
     4981 cgttatccgc atatcagcat ttgctgggaa cagagcgaca cctg
//
LOCUS       DAJTSI010000213         5018 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000213 DAJTSI010000000
VERSION     DAJTSI010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.215"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(241..486)
                     /locus_tag="OO327_003799"
     CDS             complement(241..486)
                     /locus_tag="OO327_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020160.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1263..2105)
                     /locus_tag="OO327_003800"
     CDS             complement(1263..2105)
                     /locus_tag="OO327_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HCR6020161.1"
                     /translation="MSEITASRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEVSAMTESIRGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDRELWRDLMQRS
                     GMLSLMDAQTRDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFKGLS
                     WDYKTNSPCKFGSKIIVNNLVRWDRWGFHLITGQQTDRLVDLERMLHLFSGKPIPDNR
                     ENITIRLDGHIQSVQGKERYEDEMFIIKYFKKGSAHITFKRLELIDRINDIIARYFPS
                     VLSA"
     gene            complement(2190..2387)
                     /locus_tag="OO327_003801"
     CDS             complement(2190..2387)
                     /locus_tag="OO327_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020162.1"
                     /translation="MKLLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            complement(2407..2895)
                     /locus_tag="OO327_003802"
     CDS             complement(2407..2895)
                     /locus_tag="OO327_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020163.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLSELINKVSENGYS
                     LRVVDKSDQHATSTPPPLTTLTCIRCSTAHITETDNAWLYSLSHQTNDDGETEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSRTFRRLVVTLTRRYGVSLIHLDAGAECLPGFPTF
                     DW"
     gene            complement(2892..3269)
                     /locus_tag="OO327_003803"
     CDS             complement(2892..3269)
                     /locus_tag="OO327_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR6020164.1"
                     /translation="MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRDNYRTVNNITRGKHPEAKQ"
     gene            complement(3316..3693)
                     /gene="cbtA"
                     /locus_tag="OO327_003804"
     CDS             complement(3316..3693)
                     /gene="cbtA"
                     /locus_tag="OO327_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HCR6020165.1"
                     /translation="MSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAAPATTV"
     gene            complement(3773..3994)
                     /locus_tag="OO327_003805"
     CDS             complement(3773..3994)
                     /locus_tag="OO327_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR6020166.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSTDTYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(4081..4557)
                     /locus_tag="OO327_003806"
     CDS             complement(4081..4557)
                     /locus_tag="OO327_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR6020167.1"
                     /translation="MQQLSFLPGEMTTRERSLILRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVPPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(4573..>5018)
                     /locus_tag="OO327_003807"
     CDS             complement(4573..>5018)
                     /locus_tag="OO327_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR6020168.1"
                     /translation="SACAPETDLQQLVATLVPDEQRISFWPQHFGLIPQWVTLEPRVF
                     GWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGNRAEMSPEAAG
                     IAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
BASE COUNT         1163 a         1221 c         1335 g         1299 t
ORIGIN      
        1 aagtatgtca ggcttttttc cgtaatgctc taacttccac gcattggttt ctgacatcaa
       61 ataaggctat gactttctct cccttttaat ctcattctga tctaaaacca tatcttaaaa
      121 aaatgcatgg gtattttaat tattttgatg ttgatctaac gcactcaagg accattttat
      181 taccgattat ccattctatt taactgttgc gatataaatg tttgttttag gtgctttaat
      241 tcagtacaag tcatcaaacc gtaccagacc ctgactgtca gtgacaccga caggtggggg
      301 cggcggtgtg gggtcaaact gagtgtgtct gcctctggcc cacagagcca gctctgccgc
      361 cattgcccgt ggatgatact ccagctgcac gttgagaccg gaaactgggt tgttgttctc
      421 atccacgaac tgcagcgcca gccagtgcat ccgctgctta ccgcaggcga cttctttgta
      481 cttcattttg tgactcaggc aggtgactgt cgtgttgcct gttgcgtcca ttttgcggtt
      541 aaaccctttt aattcaattt gttaaattgc tttccacctt ttgcggggcg cgacttaacc
      601 aaaatattca ctgttatcat aatatgcaat gggctggagg ttgcaggtca tttagggatt
      661 ttctgtaagc cccgttttgc agaacgttct atctccgatt gagatcctgt ctctcggtat
      721 taccggtagt ctggccataa tcccctatgg atcatattga tatagtaggc ataacaccca
      781 aaagttgact aaagctaaat caatcagagt gaaaaatgtt cggtttgatg ttggggggga
      841 ctgaggaggg gccaacgcag gtggtatgcg ttttacttca gaccggacaa agaccggatg
      901 tatccgtgca gtaatgaatc cgttagccag gtgctcatgt ggaaatagtg tctattgggg
      961 atttgaagtt tgacagaaaa ggccacggtg accagaaggg ggctacttag aagaaatgtt
     1021 caggctgggt ggaagaggta aagcagtgac atcagcattc aaagtgaccg gatatctacc
     1081 agaaccccag aaagcaaaaa accagcccgt aggctggttc ttctaaatag tggtgccgga
     1141 ctcggaatcg aaaatttctt ttatcatatt gatattaatg caatttgttt ggtttgatta
     1201 aaaatgtacc ctcaattata cccgcagtcc cttttgatat cgaaagggaa tcactctcgg
     1261 gatcaggctg agagcacaga aggaaagtac ctggctatta tatcgttaat tctgtcaatc
     1321 agttccagcc ttttgaaagt gatgtgtgca gatcccttct taaagtattt aatgatgaac
     1381 atctcgtctt catagcgctc tttaccctga acggactgga tgtgaccatc cagacgaata
     1441 gtgatgtttt cccggttgtc ggggatcggt ttgccgctga acagatgtag cattctttcc
     1501 aggtcgacga gtcggtcagt ctgttgcccg gtgataagat gaaaccccca ccggtcccac
     1561 ctcaccaggt tgttgacgat aattttactg ccaaatttac agggtgagtt ggttttgtaa
     1621 tcccagctca gccctttgaa gacgttgatc actccccgtt caaacacttc atctttgttc
     1681 tggtgcagtt gttcaaatgt gctcagaatg ttcgcttcgc tgatttccgg aaaattatca
     1741 tattccagtg acctgtacca tgtgtcccgg gtctgcgcat ccatcagact cagcatcccg
     1801 gaacgttgca tcagatcacg ccagagttcg cgatcaagat tgcgtgtgat ggctttcatt
     1861 gcggtttccg gtttctccat cagccagcaa ccgcagcgaa aatcctgttt catggcccag
     1921 tccagagccg ttttacctct aatactttct gtcattgctg atacttcagc cagttgtcgg
     1981 ataagtgtgt cgatttgcag caatgtactc tttcgtacag ccagaatgtg gcggatactg
     2041 gttgagcaga taacgtccgt atgcccgttg accacttccg gacgagaggc tgtgatttca
     2101 gacataatcg atattccttt ttgcagataa acgaaaacgc cagccgtatt caggctggcg
     2161 tcagtggaat gaagtctggt gagtaagact caccggttct gttgcagaaa ctgcagctga
     2221 cgcagggtac ggatatcttc cctggcctgt tcaatacagg gcagcagtgc tgccgaatcc
     2281 gttttatcct catcgttgtc gaagataatc cctgattcgc agtcaatatt gtcctgcagc
     2341 cacgcaatca gaatatccag cgccgtttct gtggttaata atttcatcca gtgtcttttg
     2401 tcctgattac cagtcgaaag tggggaaccc cggcagacat tctgcgccgg cgtccagatg
     2461 aatgagactg acgccataac gccgggtgag agtgacgacc agacgacgga acgttctgga
     2521 caggcccagg cgtttaagcc gcagaaccgg gtacgaccat gcatcggtac gtaacagata
     2581 gccgctgcct gtaaaatgaa tccattctgt ttcgccgtcg tcattagtct ggtgtgacag
     2641 cgagtacagc caggcgttgt ccgtttccgt gatatgtgcg gtgctgcaac gtatgcaggt
     2701 aagggttgtt aacggtggtg gtgtactggt tgcgtgttgg tcggatttat cgaccacgcg
     2761 gagtgaataa ccgttttcag agaccttgtt aatcagttca ctgagattaa caccatcgac
     2821 gtcaacgaca atgcgcccca tgttcagtgc ctgcacgtta acgctgtcgg cttccagcgt
     2881 cagggccagt ttcattgttt cgcttccgga tgcttccccc gggtaatgtt atttaccgtt
     2941 ctgtagttat cgcgggtcat caggccggtt gcccggcgtg cccggagaat atcaatgctg
     3001 ttgattaact gcgacggggc tcctgcgctg aatcccggct ggtcggtacg taccagcgcg
     3061 tatttttcaa cgagaaagtt caccgcgtca cacagtgaaa ttcctgcctc aatatgctgc
     3121 tcaatcacac gttcatcagc gaacggtgtg tcattcagcg tgaggccata gtgctggtcc
     3181 agcagtcggg tgagcagtgt ctgccagatg gtgatgggag acgggcagcc cgatgcctcc
     3241 cgtacgtgag tgtcgggtaa tgtattcatc ttaagttctc tgtaacaggt ggttataaag
     3301 gggagatatc cggtgttata cggtggttgc gggcgctgcc ggtgtcggat aaacagccag
     3361 ataaacgtaa ccacaggagc cgagggtgtc ggcttcgcag gtcagccctt tcgcatacag
     3421 tgtgacggta tgctgatggc ggggattcag ttcaccgcca gtgagcatga gttccagttg
     3481 tttcatcagc agcggaaagg cctggtccag gtggtacgca tctacgtcgc tgaacctgcc
     3541 tctgataccg gcgcggtctg caaggtaatg caggcggtta ccctcctgta ccagtcgggc
     3601 cccgaaacag ggcgtcacgg tgcagggcag cccccaccag gggcggtcgt gattgtcgtc
     3661 gggaggcgtt gtcccgggga gtgtgtctga cacgataaag tcctcacaga aaatcggtta
     3721 agaatggtcc ggtagtggcg ataattctgc tcatcagaat tcccttccag actcagttca
     3781 gggtgacgct catcagccgg acatacggac caaaactgtc cttacggcgt tctgcaaaca
     3841 cagccagtac accgggaata tcctgcactt cacggccggt atatgtatcc gtgctaccgt
     3901 gccagcggta tttaccggta cagaacggaa aaagacggga tgcaggatgc tgacggtgaa
     3961 tacgcatggc ttcaccacgg gtgatgattt tcatgagaat agtcctcctg aatgtgaaag
     4021 taagaaacag ctgagaaaga gaaactgaag tgatatgaag gtgattgtga cggtgacggg
     4081 ttaaagcaga ccgtgttccg caaaggagaa aacctggctg ccaccgacta tcagatggtc
     4141 cggtacccgg atatcaacca gggccagtgc ctgtaccaga cgctcagtga taaggcggtc
     4201 tgccttgctg ggggggactt caccggacgg gtgattgtgc gccagcacca cggcagcggc
     4261 attgtggtac agggcgcgtt taatcacttc ccggggatgg acttccgtac ggttgatggt
     4321 gccggtgaag agggtttcac cggcaatcag ctgattctgg ttgttcagat acagcacccg
     4381 gaactcttca cgctccagtc ccgccatgtt cagaatcagc cattcccgtg ccgcatgcgt
     4441 ggaggtgaag gccacgccgg gttcatgaag atggcggtcc agggttttca gggcccgcag
     4501 aatgaggctg cgctctctgg ttgtcatttc tccgggcaga aaggaaagct gctgcattgt
     4561 ccctgccacc attcagtcga tgatgcgcat aatggcgctg cattccggat gctgcagggc
     4621 gtaatcccgc agccggtaat aatggaccgt catggcataa cactccgtac gacaggcatg
     4681 atgactgtac gtcatcagac aggcagcaat gccggcagct tccggactca tttcagcgcg
     4741 gttaccgttc atggcattga acagtaccca tgtttcgtca tcatcgtcat ccggttcggg
     4801 tgccataaat gccccgccgt tgttcagggt gtacagattc cagataccac cgcagtagtc
     4861 ttcgcacaga cggtccatcc agccgaagac acggggctcc agggtcaccc actgtggaat
     4921 gaggccaaag tgctgcggcc agaagctgat gcgctgttca tcagggacga gggtggcaac
     4981 cagttgttgc aggtcagtct ccggtgcaca ggccgaag
//
LOCUS       DAJTSI010000214         5005 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000214 DAJTSI010000000
VERSION     DAJTSI010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5005
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.216"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(131..3043)
                     /locus_tag="OO327_003808"
     CDS             complement(131..3043)
                     /locus_tag="OO327_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020169.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     AKHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3046..3162
                     /locus_tag="OO327_003809"
     CDS             3046..3162
                     /locus_tag="OO327_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6020170.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3526..4368
                     /locus_tag="OO327_003810"
     CDS             3526..4368
                     /locus_tag="OO327_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR6020171.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4371..>5005
                     /gene="capU"
                     /locus_tag="OO327_003811"
     CDS             4371..>5005
                     /gene="capU"
                     /locus_tag="OO327_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR6020172.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLK"
BASE COUNT         1341 a         1179 c         1172 g         1313 t
ORIGIN      
        1 atggcctgcc cgtgtcagaa aatctgctgg agcaggattt ttacgccagt ggtccgaacc
       61 agaagtgggc gggagacatc acgtacttac gtacagatga agttcggtta catcctgtca
      121 gcacggaacc tcacgctttt tgagcggcac gacggatccc ccccacccag tatccgacag
      181 acttttcact gcgttcgtaa aaacccttct tccctcccag catatccact aattccggtg
      241 acatatcctg cagataagtc tgcaaatcat gccccagatc ttccggtgac tgcaactcat
      301 aaaccgcgcg ccacacatca cccttcctta ttttaagccc cgggttaaga atatactgtc
      361 cgtggttcag cctcagaaaa agcttttttc cataaggatt tttgctgtta acttcatgtt
      421 gagagagctg gctgctgata taactccttc gtttgcggcg ttccggcagc aacgcttcag
      481 gaagatgaac cacacactct gccagtcgtg ccgcactgaa ggcttccgaa aaaaacagag
      541 cacttcccag atgcgtgtgg aacagcgcct gcatcaggat caccagaaac attgccatct
      601 gccggttgtc cagtttgatg aggcgtcctt ccacctgcag tgacagactt tcaggtttca
      661 gcagggtata caactgcgca cttttctgtt gcgcataacg gggcgtactg accgccgctg
      721 acagaacttg atgataagcc gccagcccgt cattaccggt cagcaaggga tctgcaccac
      781 gctccagcag taactgaacc agtgcaatat tacctgcccg ggctgctgac atcagcggag
      841 taagattaaa cggcgaacgg tgatcaacac cataacgttc aatatcccgc aacacggcaa
      901 ccgggttatt cattgagtat ggccggaaat acttcagctc cagtaactgc agagtcttcg
      961 ttctgggctg ccttgtgggc ttaaaatctt cccggtacag tgcattatac agcgggaaca
     1021 gggaataaat cagactgtac tccagcgcct gcagttgtat ggttttatcg gctgtacctg
     1081 cgaggatatg ctgtttccac tgtcgtgcat cctctgcggt gataatctgc catggcatct
     1141 cagatgtctg caggatccgg ctacggatgg tgtccgcctg ctcctgcttc ccctgttttt
     1201 caagcagatg tgcggttttc tgccactcat cacggctgga aatctcttcc tgcagattca
     1261 gaggctcttc ctgatgcgtg agcgcaagca gactccacag aggatgctcc acctgctgtt
     1321 caacaagata cacattgtgt ttagcacggg tgagcgcaac atacagcgcg ttggtaaaaa
     1381 atttatagat ttccgctgaa cgatcctgcc tgtcccgcgg acgggaataa cgggcctcgc
     1441 cctcaagatc agccggtgta agaccctcgc aaatatcatc aaatgcctgc cgggcagcgc
     1501 ttactatatt gtaaaggatc accgtttcgt attccagccc cttaacctcc tgaaccgaaa
     1561 agaccagcgg cgtactgaac cagcatcgcg cctgtgcttt ctgttccgga tgcatcacaa
     1621 tgaccgctac acgattcgaa agtgaggttt tggcattcag ttcctgcttt gtctcttccc
     1681 tatcgtccag caaccggatg gtcccttctg cctgaccgca gcttctgaca aaatggtgac
     1741 tttcccgatc aatagcacta aagcggacct gtttgagtct cagcaaccgg ttagccagtg
     1801 cagtgacgtg gtgcccgtta cggtaattcg cctgcaggat attaacggtt gtgtcgtttc
     1861 cctgttgctg ccggaaaaag agacttttga gcgaactcca tgagaaaaag ctcggatgaa
     1921 ctatctgatt ggcatcacca cagagtaaaa actgaccggg atgacgtagc gttttcagta
     1981 ccagttgcag ttgaacaggc gtcatatcct gaacttcatc aacaaaaatg acatcataac
     2041 ggggagttgc caacgaaaga tactgatgac tgagcagatt actgtcatac tgatgcgact
     2101 gtttcagcca tgcgatataa cgttcaaaca ggacatagac cgtcgggcgg tcctccatgc
     2161 catagagcga ctgccgtatc cccagtgaca gatacgcctc ccgggacagc ggcccattcc
     2221 cggaggcaac cgcaccgatc acgcccctga actcctcata cagggtatgg gcagccccca
     2281 gtgcggcgat agccctgttg cggggcagcc agtcgctgaa cgcactgaaa gtcacctccc
     2341 tgccttccgg aatgcgcaga gtttccagaa attccgtcag cgaaagaaag tcaatattct
     2401 gtacctcact cccttcccct gaagcgtcat agagagtacg cgctttttcc accagaaatg
     2461 aggaaaggct gagatacagt atatcaccag cagcctgctt cattttttcc agtaccagcg
     2521 ccgtcttacc acttcccgcc gagccaacaa tcaccagcgg tagcgggtgt tcgtacagtg
     2581 cctgctgatc atcatcaaag gacagcattt tatccaggcg gtggaaacgc ccatgagacg
     2641 gattgatata cgtcagcgct tcagtcgcga tatcaacagg atccggcact ggttgttgtt
     2701 gtaagcgccc ttcatcaatc accacgtttc tgcgaagaaa acgtgaagtg ttataggcat
     2761 gattgcgaat atattccaga acaaggcaga tagtttcacc gtggtagcga tacagcgaaa
     2821 acagtaatcg gtcccggata ctgagccggg cgcggtaaag attggtgtca attttacgaa
     2881 catcagcctg aacaaagtta tcggtggcaa tagcctgtgc aaatttttta taacccggga
     2941 tcgtgtcagg attcagagta tgataaagac agattttcat ggctgccggt ttccgtcgtg
     3001 caaaaaacat gctgatttta cgaaggaatg ccttactgaa caaccttgcc cggaataccc
     3061 cccgaaatca gatggaacaa tcagaaaaat gctctgcagt tatggaataa agaaggtggg
     3121 gtggtacgcc gggagggcga tccgctggaa atggcgcagt agctgcaggc ctgtcatgat
     3181 gcaggaatag aagttcgtgt acaggttaat gaaagcgtca ccccgtaaat aaactctgcg
     3241 ctgaaattcc tttttttcat tggagtacct gtgttgttct gaggtgaaca tatcacctct
     3301 gttcaggtag ccaaattcag tgtgccacct caaggttctt agtgagtcgt ttttaaaaga
     3361 tatgataata tatcattgtt tatgaattgt tgatgtttaa tttagtaaat tatatcaaat
     3421 atgccatggg tttgctatgt tattcatcca gtatctggaa ataaaatgca ccagctttat
     3481 tgggttttcc ttttgtctct gcttgctagg tgccagaaac tttaaatgtt aaacgaaggg
     3541 ggcattttgt tcaaagccaa tcatgttccg gtattaatgt atcatcatgt cagtcattgc
     3601 cccggactgg ttacactttc tcccgtgaca ttccggaagc aaataaaatg gttggctgaa
     3661 aacaactgga aaacactgtc atcagatgaa ctggagtttt tttatcgggg cggaaaactc
     3721 ccccggaaaa gcgttatgtt gacatttgat gatggatacc ttgacaactg gtttcaggta
     3781 tatcctttat taaaagagtt taatctcaaa gcacatattt ttttaattac cggttttatt
     3841 gggaatggtc cggttcgtca ctctcctgga aaggaatatt ctcatcgcga ttgcgaacat
     3901 caaatagcga cagggaatgc tgataatgtt atgcttcgct ggtctgaagt aaacgaaatg
     3961 ttgcaatctg gtctggtcga atttcatgtt catacgcata ctcatacacg ctgggataaa
     4021 aagttcagtt cacgagaaga acagtgtaaa catcttcggc aggacttgtt gagcgggagg
     4081 gaatatttga aagaaatgac aggaaaatgc agtaaacatt tgtgttggcc ggaaggttat
     4141 tataacaagg attatattca ggtcgctgaa gaacttggat tctattattt atatacaaca
     4201 gaaagaagaa tgaatgctcc tgctaaagga actacacgga taggaagaat aagcaccaaa
     4261 gaacgggaaa gttgcgcatg gctgaaaaga cgactttttt attatacaac cccgtttttt
     4321 tcatcgttgc tggcctttca taaggggcca agattaccgg atgattaata atgaatatac
     4381 tatttacgga atcatcaccg aatataggtg gtcaggaatt acaggctgtt gctcaaatga
     4441 aggccctgaa gaaaatgggg cattcagttc tgcttgtctg cagggaaaac agcaaaattg
     4501 cttttgaagc cagtaaattg gggattgata tcacattcgc gttatttcga aacagtcttc
     4561 acatccctac tgcatggaga ttactcggaa tagttcatgg ttttcagccc aatgcaatcg
     4621 tttgtcacag tgggcatgat agcaatattg ttggtttagt acggttattc actaggaaac
     4681 atccattcag aattatcagg caaaagacat atttgacacg aaaaacaaaa gttttttcaa
     4741 taaatcattt ttgcgatgag gtgattgttc ccggaacaag tatgaagaca catctggagc
     4801 aggaaggatg tcgaacccgg gttactgttg tgcctccagg ctttgatttc cagaaattat
     4861 acgttgattc ccgaaacagt ttgcctccaa atgttctttc ttggctggcg tcccgaaggg
     4921 gatgccccgt tattgctcag gtaggaatgt tgcgcccgga aaaagggcac gaatttatgt
     4981 tgaatttact gttccattta aaaat
//
LOCUS       DAJTSI010000215         4973 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000215 DAJTSI010000000
VERSION     DAJTSI010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.217"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(186..770)
                     /gene="kptA"
                     /locus_tag="OO327_003812"
     CDS             complement(186..770)
                     /gene="kptA"
                     /locus_tag="OO327_003812"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCR6020173.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="OO327_003813"
     CDS             1020..1703
                     /locus_tag="OO327_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCR6020174.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="OO327_003814"
     CDS             1700..2161
                     /locus_tag="OO327_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCR6020175.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="OO327_003815"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="OO327_003815"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR6020176.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="OO327_003816"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="OO327_003816"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR6020177.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="OO327_003817"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="OO327_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCR6020178.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="OO327_003818"
     CDS             complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="OO327_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCR6020179.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         1176 a         1305 c         1239 g         1253 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagtc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tggctgataa ggtttttcag
      961 ttccaggaat tttcctggct caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct tttttcccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg cggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc cggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattcc
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcagcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggt gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagca
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtgag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaaa cgctagtata ttctgaatat tccgaatagt tac
//
LOCUS       DAJTSI010000216         4909 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000216 DAJTSI010000000
VERSION     DAJTSI010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4909
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.218"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            66..422
                     /locus_tag="OO327_003819"
     CDS             66..422
                     /locus_tag="OO327_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCR6020180.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            522..1727
                     /locus_tag="OO327_003820"
     CDS             522..1727
                     /locus_tag="OO327_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6020181.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            1910..3182
                     /locus_tag="OO327_003821"
                     /pseudo
     CDS             1910..3182
                     /locus_tag="OO327_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            3194..4060
                     /gene="ydiB"
                     /locus_tag="OO327_003822"
     CDS             3194..4060
                     /gene="ydiB"
                     /locus_tag="OO327_003822"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR6020182.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            4091..4849
                     /gene="aroD"
                     /locus_tag="OO327_003823"
     CDS             4091..4849
                     /gene="aroD"
                     /locus_tag="OO327_003823"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR6020183.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
BASE COUNT         1235 a         1107 c         1136 g         1431 t
ORIGIN      
        1 caagccgtca gcaggctgat aatgttattt atattttatt gtttatttca tgcagccatt
       61 acacgatgaa cgcttatgaa ctccaggcac tccgccatat ttttgccatg actattgatg
      121 aatgcgcgac ctggattgcc cagaccggtg acagtgaaag ctggcgtcag tgggaaaatg
      181 gcaaatgcgc cattcctgat tgtgtagtcg agcaactgtt ggctatgcgt cagcaacgaa
      241 aaaaacatct tcatgccatc atagagaaaa taaataaccg catcggcaac aacaccatgc
      301 gttttttccc cgacttaacc gcgtttcaac aagtctaccc tgatggcaat tttattgact
      361 ggaagatcta tcaatcggta gccgctgaat tatatgcaca cgatctggag cggctttgtt
      421 aatttttcca cagaaaggaa ttgtcgttgt tacaacaata atgaatggat actgacacaa
      481 catcgctaca ctttttaaag cacctttgct aagtagaacc tatgaaaaat ccctattacc
      541 ctaccgcact tgggttgtat tttaattacc tggtgcatgg tatgggcgtc attttgatga
      601 gcctgaatat ggcctcgctg gagacacttt ggcagactaa tgccgcgggt gtctcgatag
      661 ttatctcatc gctgggcatt ggtcgattaa gtgtcttgct ttttgcagga ttattatccg
      721 atcgctttgg tcgccgccct tttatcatgc tcgggatgtg ctgctatatg gccttctttt
      781 ttgacatcct gcagaccaat aacatcatta tcgcttatgt ttttggcttt ctggcgggaa
      841 tggcaaacag ttttctcgat gcaggcactt atcccagttt gatggaagct tttccacgct
      901 cacctgggac agccaatatt ttaattaaag catttgtttc cagcggacaa tttttattac
      961 cgctaatcat tagcctgtta gtgtgggctg aactgtggtt cggttggtcc tttatgattg
     1021 ctgcaggcat tatgtttatt aacgctctgt ttttataccg ttgtacgttc ccaccccatc
     1081 cgggtcgtcg cttacctgtc ataaagaaaa ccaccagctc tacggaacat cgctgttcaa
     1141 ttatcgattt agccagttat accttatatg gctatatctc aatggcaacg ttttatctgg
     1201 ttagccagtg gctggcacag tacggacaat ttgttgcagg catgtcatac actatgtcga
     1261 tcaaactact cagtatctac accgtgggtt cgctgctttg tgtatttatt accgctccac
     1321 tcattcgtaa taccgttcgc ccaacaacat tactgatgct gtacaccttt atctcattta
     1381 ttgctctgtt taccgtctgc ctgcatccca cattttatgt ggtgataata tttgcttttg
     1441 tcattggttt tacctctgct ggaggtgttg tgcaaattgg cctgacgtta atggctgaac
     1501 gtttccctta cgctaaaggt aaagctacag ggatctatta cagtacgggc agtattgcga
     1561 cctttactat tccgttgatt acggctcatc tgtcccaaag aagtattgcc gatattatgt
     1621 ggttcgatac tgccatcggt tttttactgg cactgtttat cggcttacgc agccgcaaaa
     1681 aaacgcggca tcactcgcta aaggaaaatg tcgctccggg tgggtaatgc aatattcttt
     1741 tcaggtcatg caagatctta cggataaata actctttctg cgctaactaa ggaaaatcgc
     1801 gatcaaaaac aaactatgac atgcaatatt cctggaaaca taaactttat gccatgtacc
     1861 cagggaaaat catcttcagt atagtaatta tgtaaaccgt cggagaacaa tacgtacggt
     1921 aacgaaatta tctttcagca cgccatttat cctggctgtt ctttgtattt acttcagcta
     1981 tttcctgcac ggcattagtg ttattacgct tgcccaaaat atgacatctc tgtcagaaaa
     2041 gttttccact gacaacgcgg gcattgccta tttaatttcc ggtatcggtt tggggcgatt
     2101 gatcagtatt ttattcttcg gtgtgatctc cgataagttt ggtcgtcggg cggtgatatt
     2161 aatggcagta ataatgtatc tgctattctt ctttggtatt cccgcttgcc cgaatttaac
     2221 tctcgcctac ggtctggcag tgtgcgtagg tatcgctaac tcagcgctgg atacgggtgg
     2281 ctaccccgcg ctcatggaat gctttccgaa agcctctggt tcggcggtca tactggttaa
     2341 agcgatggtg tcatttgggc aaatgttcta cccaatgctg gtgagctata tgttgctcaa
     2401 taatatctgg tacggctatg ggctgattat tccgggtatt ctgtttgtac tgatcacgct
     2461 gatgctgttg aaaagcaaat tccccagcca gttggtggac gccagcgtag ctaatgaatt
     2521 accgcaaatg aacagcaaaa cctttagtct ggctggaagg tgtttcatcg gtactgttcg
     2581 gtgtagccgc attctcgacc ttttatgtga ttgtggtgtg gatgcccaaa tatgcgatgg
     2641 cttttgctgg tatgtcagaa gctgaggcat taaaaaccat ctcttattac agtatgggct
     2701 cgttggtctg tgtctttatt tttgccgcac tactgaaaaa aatggtccgg cccatctggg
     2761 ctaatgtatt taactctgcg ctggcaacaa taacagctgc cattatctac ctgtaccctt
     2821 ctccactggt gtgtaatgcc ggcgcctttg ttatcggttt ctcagcagct ggcggcattt
     2881 tacagctcgg tgtttcggtc atgtcagagt ttttccccaa aagcaaagcc aaagtcacca
     2941 gtatttatat gatgatgggt ggactggcta actttgttat tccgctgatt accggttatc
     3001 tgtcgaacat cggcctgcaa tatatcattg ttctcgattt tactttcgcg ctgctggccc
     3061 tgattaccgc aattattgtt tttagccgct attaccgcgt ttttattatt cctgagaatg
     3121 atgtgcggtt tggcgaacgc aaatttagca cccggttaaa cacaattaag catagaggtt
     3181 aaaggagtta attatggatg ttaccgcaaa atacgaattg attggattga tggcttatcc
     3241 tatccgccac agtttatcgc ctgaaatgca gaataaagcc ttagaaaaag cgggattgcc
     3301 atttacctat atggccttcg aagtggataa cgatagcttt cccggagcaa ttgaaggatt
     3361 aaaagccctc aaaatgcgcg gaactggtgt gtcgatgccg aacaaacaac tggcgtgtga
     3421 atatgttgat gaattaacgc cagcagccaa actggtgggt gccatcaaca ccatcgttaa
     3481 tgatgatggc tatctgcgtg gctataacac cgacggcacg ggccatattc gcgccattaa
     3541 agagagcggt tttgatatca aaggcaaaac gatggtgctg ttaggggccg gaggtgcctc
     3601 aacggcgatt ggcgcgcagg gggcaattga aggtttaaaa gaaattaaac tctttaaccg
     3661 tcgggatgag ttcttcgata aagccctcgc cttcgcgcag cgggttaacg aaaacaccga
     3721 ttgtgtcgtc acggataccg atctcgccga tcagcaagcc tttgctgaag ccctggcttc
     3781 cgccgacatt ttaaccaatg gcacaaaagt aggtatgaaa ccccttgaga atgaatcatt
     3841 ggttaatgat atcagtctgt tacatccggg actgctggtc actgaatgcg tgtataaccc
     3901 gcatatgacg aagttattgc agcaggcaca acaagctggt tgcaaaacga ttgatggata
     3961 cggcatgttg ttgtggcaag gggctgaaca gttcacgtta tggactggca aagatttccc
     4021 tctggaatat gttaaacagg tcatggggtt cggtgcctga caggctgacc gcgtgcagaa
     4081 agggtaaaaa atgaaaaccg taactgtaaa agatctcgtc attggtgcgg gcgcacctaa
     4141 aatcatcgtc tcgctgatgg cgaaagatat cgcccgcgtg aaatccgaag ctctcgccta
     4201 tcgtgaagcg gactttgata ttctggaatg gcgtgtggac cactttgccg acctctccaa
     4261 tgtggagtct gtcatggcgg cggcaaaaat tctccgtgaa accatgccag aaaaaccgct
     4321 gctgtttacc ttccgcagtg ccaaagaagg cggcgagcag gcgatttcca ccgaggctta
     4381 tattgctctc aatcgtgcag ccatcgacag cggcctggtt gatatgatcg atctggagtt
     4441 atttaccggc gatgatcagg tcaaagaaac cgtcgcctac gcccacgcgc atgatgtgaa
     4501 agttgtcatg tccaaccatg acttccataa aacgccggaa gccgaagaaa tcattgcccg
     4561 tctgcgcaaa atgcagtcct tcgacgccga tattcctaag attgcgctga tgccgcaaag
     4621 taccagcgat gtgctgacgt tgcttgccgc gaccctggag atgcaggagc agtatgccga
     4681 tcgtccaatc atcacgatgt cgatggcaaa aactggcgta atttctcgtc tggttggtga
     4741 agtatttggc tcggcggcaa cttttggtgc ggtaaaaaaa gcctctgcgc cagggcaaat
     4801 ctcggtaaat gatttgcgca cggtattaac tattttacat caggcataag caataatatt
     4861 tcggcggaaa taccctcccc cgccgaaata aaaaaagtat ttttaaccg
//
LOCUS       DAJTSI010000217         4897 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000217 DAJTSI010000000
VERSION     DAJTSI010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.219"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(403..1992)
                     /locus_tag="OO327_003824"
     CDS             complement(403..1992)
                     /locus_tag="OO327_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6020184.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            2377..3408
                     /locus_tag="OO327_003825"
                     /pseudo
     CDS             2377..3408
                     /locus_tag="OO327_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(3495..3971)
                     /locus_tag="OO327_003826"
     CDS             complement(3495..3971)
                     /locus_tag="OO327_003826"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020185.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(3964..4728)
                     /locus_tag="OO327_003827"
     CDS             complement(3964..4728)
                     /locus_tag="OO327_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR6020186.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGIAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
BASE COUNT         1501 a          949 c          832 g         1615 t
ORIGIN      
        1 acttgatata tcatcaccta taccatttgc aatataccca aacagttctt tagtgaagtt
       61 ggtaacatgc tcttgcacca tatcatttgt tgcgattgtc gttgtactgc cattagtgcc
      121 tgccacagtt ccaattttca tcttttacct catattattc accgcgagga atacatagtt
      181 aacattttat cttatgacat acacctcata ttcgatgggg aaataattat attttgcaca
      241 aatcattcag ccttaaagca taatatgaaa tttcttatct catagcagag aaagtcccta
      301 aatattgtag aggcgtcatc agtacatacc tctacaatcg attaagaaaa cctatgaaat
      361 actggcaaaa ttaaaaagag ataccacagc actcatgaag ttttattgag gttgaaaaca
      421 actaagccct gggacacctt ttttatcacc acgttcgtta ggtgctatct tccaagaatt
      481 tagccccatg gtataattat caattttttg atgctggtct acccctaatc cataatggcc
      541 aaatttctgt ttagtttttt tgagcgtctg tccacatttg aagatatctt caggttttgg
      601 tctgaaagca gtagtgttat taaaataatt ttcatataca ggtgtgggtg ctatagcatt
      661 gcttctaatt agatggagaa gatgatcatc catgtgttgt ttatgttttt tattacatat
      721 atttgctaat tcctcaggat caattgggtc tttcatctga ttaaatggaa cctgcaaagc
      781 accgagacaa taacattgat tttcttcatt tcttattatt ccgtgatgac tggttaaaac
      841 tttagtttct gagttataat tacaaacatc gagcgtttta attaagttat acgtatcacc
      901 ggcagagagt ttataatttt ctagtcgtgc agtatagtta ccattaaaat tgatttcatg
      961 atttccagcc agaacgatga catttttatt aatacggcta tctttattac cctccatatt
     1021 tcgcatagaa tttagcaaag taaggatata tttatcacca aaaatagtac taaatctatc
     1081 gcctgtatga tccccaagga agatacaagg ggttatcgca tcgtccttta aaaaaacatg
     1141 ttgatgcaac tcattcaata tcatgtctct cgtatttaat aaattgtaga gcaatttatt
     1201 atttttatgt atgtatgcag atgaagccat gtcttcgtgt tttaataatt cacataataa
     1261 actccaaccc ttttccctga tgcctatatg ccctgaaacg agcaatgaat aaagtacagc
     1321 gcctactctt ccatctgtat cgccaaaata agccgtgttg gcattttgtg ggttaatcac
     1381 accacattgt ttataaaagt ctattttaga aaacacagat ttaacatagc tatcaaactt
     1441 atccttagga aacgtctctt tagagacatc ccaacgccag tcagctttaa aattaccgct
     1501 aagaagagta aagaaatttt caggtatgat agaaagccca ttaactttat aaattatgtc
     1561 tgatttatct ccggagaaat taataaaata ataaccatcc ttacactcaa tcttaactgt
     1621 aatgggagac gataatttta tcacatggtt attgtctggt ataattgtaa agataaacgt
     1681 gttagtagta ttatcggcaa aactattata ttcttgaata tctatagatg gctcactatt
     1741 gtaaaaccat attttctcca tctgaagact atgttcattt accctccagt gaccatgttg
     1801 caggaaatag atgatatgca tgccaaaatc gttatctatt tgcgaatcat tatccagctt
     1861 atcaatttca actatctttg aacgatgtgt tttatcaacc ttgctatggt gttgatgatt
     1921 attaacttta tctgaatgct tatctttatg gtttgaaggg atgtacttaa atatattgtc
     1981 aattgctttc ataaataacc cgcgatattt ttgacctgtt agtaaaaaat aaatgataca
     2041 tgtctgaaag acatatttac gacgatataa aacgataata cttatatgtt cgctgatttt
     2101 aagcaccgag actatccggt cttttactaa ataatggaaa acaatccatc cctggaagac
     2161 aattgtaaaa aaaaacaaca gaataagaat taatcttagg ataaatattt atttatcatg
     2221 gccttttgac cgctaaaaaa tggcactcgg cacagtaaac cccgctatag ttatgctatg
     2281 catttgtctt taatgatatc tcgtcgttat attaacaggc tgaaattatc attaatgcac
     2341 atttcaatat tagcagggat acctgaggag tttaaagtga acatatatat cgggtagctt
     2401 ttcaaattaa tccctttgat tataggctta atttgcatcg cgttaggtgg ctttgtgctg
     2461 gaaagttcag ggcaacgtca cgggtcatgt gctgatttct ctggcggcca tatgcctggc
     2521 attattcact accgcattta ttatcatttc gcaactcacg cgcggcgtta atacgtttta
     2581 caatacattg ttccccatta ttggctatgc ggggtcaatt atcaccatga tatggggttg
     2641 ggcactgtta gcaggtaatg atgtgatggc agacgagttt gttgccggcc atgttatttt
     2701 tggcgttggt atgattgccg catgtgtatc gacggtggca gcgtcgtccg gtcactttct
     2761 gctcattccc aaaaatgcag cggggagcaa gagcgacgga acaccggtac aggcttattc
     2821 ttcattaatc ggtaattgcc tcattgccgt tcccgtttta ctcaccctgc tcggtttcat
     2881 ttggtctatt acgctgttac atagtgccga cataactccg cattatgtcg ccttgggtta
     2941 accgcaatct gtgcctgtct aattggccta gtggccacaa tcgtccatca aacacgtaat
     3001 acgttctcat ccaaagagca ctggctgtgg tgttattggg ttatttttct cggctcagtc
     3061 acggtgctgc aggggatata cgtcttagtc agttccgatg caagcgcccg actggctccc
     3121 ggcattattc tcatttgcct cggaatgatc tgttacagca tattctcaaa agtctggcta
     3181 ctggcactgg tctggagacg tacctgttcg ttagccaaca gaataccgat gataccggta
     3241 tttacctgtc tgttttgctt gtttttgtca tcattcctcg cggaaatcgc acaaactgag
     3301 ctggactact ttattccatc tcgggttctg gttggattag gcgcagtatg ttttacattg
     3361 ttctctattg tttcaatatt agaggccggt tctgcgaaaa aaaattaaaa tttacataat
     3421 cccagcgtta ttcatatctt aacgctggtt ttttacgaac ttagtgaaat aatgtagaat
     3481 ataaaattat aattttatat tggagcacat gtgttaatga tcaaccccgg gatactaaaa
     3541 atatagacat tattaaactt gcttagcctc gcattgcctt tacctgttaa agatgcatcg
     3601 agaaaattga gtctgaagtt ctcatccttt atctgatttt ccagtcccga aactttggcc
     3661 ttagcattac ctgagatccg tccgttacta tctattttgt aggtaaaaaa atacgaatgg
     3721 gaaaatttcc tcggtgtatc ttcatcagtt aagccaacaa tcgtaaaact accgctacca
     3781 tcttttttta aaatgactga aaggttagca gagtagtata aagaatgatc tgttttagca
     3841 tttcttgtta acgtcgacgt acattcaaga gacgttgtgc taccatcacg agccttccag
     3901 agagcaaaaa aacacacctg tagcaatgtg aaaacaagta ttaatataaa atacattttc
     3961 ttattagtca tataatagca cctctgaaca aattgcataa gtttcgtcat tagaggattt
     4021 tatacatctt gataaatacc gctgcccttc cccaagcaaa taatgcatac tatttacatc
     4081 atatataaaa tttgcacctg gcacacaggg taaatgcttt ttcgaaattt cagcggcctc
     4141 tatttgagat ttagcgatgc caatctcgtt aagattctct gaaagatata aatcacaggt
     4201 gtcaatctta cctgatttaa ccaatggata atccgttata ctgtaggtat ctttaaaagc
     4261 cacggcaaac atttcaataa taacaattac taccacaagc gaaatcaaaa tgcgaaaaag
     4321 aggaatactc cttttgcgct ctactttttc aacaacatct tcttgtaaat gagaatcatc
     4381 ataagattgt ttacttattg ttatgttttc actgatgtaa aagcctactt tgggtatcgt
     4441 aagaatcaca ttttcttcaa caccaatatt tttaaaactt ttgcgtaaca gggatatata
     4501 ttggttcaat gtgttgtttg acagatcaaa tgaaaatctc tccaaaacgc tatcaatgat
     4561 tgagttcctg tcaaccgctc caccattact ttccagtaac actgccaaaa tacaccccgc
     4621 cgtagctgga aaatttatct catcctgggt atttacattc gagagcgagc atttgttcgg
     4681 atcaaaaata atcgcgtcat tgataatgaa aaaacatctc cgctccatgc aggcaacaac
     4741 acaaaataag attttgtcat tttagcctat tcctggtgga actgacacaa gaaactaaaa
     4801 tacaaatata accctgaaaa agtcatataa aacttatatc cacagaataa aaaatcaaac
     4861 atgtcagttt tatcaatata aagtatcgca aggcgaa
//
LOCUS       DAJTSI010000218         4844 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000218 DAJTSI010000000
VERSION     DAJTSI010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4844
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.220"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..309
                     /locus_tag="OO327_003828"
     CDS             <1..309
                     /locus_tag="OO327_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR6020187.1"
                     /translation="LLKLVNNRSTGSDVDRQKLQEKVRSSLNRLRRLGMVWFMGHDSS
                     KFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQLNDETEESQPDSGEEE
                     "
     gene            309..4769
                     /gene="mukB"
                     /locus_tag="OO327_003829"
     CDS             309..4769
                     /gene="mukB"
                     /locus_tag="OO327_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR6020188.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
BASE COUNT         1158 a         1241 c         1428 g         1017 t
ORIGIN      
        1 ctgctgaaac tggtgaacaa ccgttcaacc ggttcagacg ttgaccgtca gaagttgcag
       61 gagaaagtac gttcttcgct caaccgtctg cgtcgtttag gcatggtgtg gtttatgggc
      121 cacgacagca gcaagttccg cattaccgaa tcggtgttcc gcttcggagc cgatgtgcgt
      181 gctggcgacg atccccgtga agcacagcgt cgcctgattc gtgatggcga agcaatgccg
      241 attgaaaatc atctgcaact caacgatgaa accgaagaga gtcagccaga tagcggagag
      301 gaagaataat gattgaacgc ggtaaatttc gctcactgac gctgattaac tggaacggct
      361 tttttgcccg aacttttgac cttgacgagc tggtcacgac gctttccggc ggtaacgggg
      421 cgggtaaatc caccacgatg gcggcgttcg ttacggcgct gatccccgac ctgaccctgc
      481 tgcatttccg taacactacg gaagccgggg ccaccagcgg ttcgcgcgat aaaggtctgc
      541 acggtaagct gaaagcgggt gtctgttatt cgatgctcga caccattaac tcgcgccacc
      601 agcgcgtggt ggtcggtgtg cgtctgcaac aggttgccgg acgcgatcgt aaagtggata
      661 tcaaaccatt tgccattcag ggactgccga tgtcggtgca gccgacacag ctggtgaccg
      721 aaaccctgaa cgaacgccag gcgcgcgtgc tgccgcttaa cgagctgaaa gacaagctcg
      781 aggcgatgga aggcgtgcag tttaaacagt tcaactccat taccgattac cactcgctga
      841 tgttcgatct gggcatcatc gcgcgtcgtc tgcgctccgc atctgaccgt agcaaattct
      901 atcgtctgat cgaagcttcg ctgtatggcg ggatctccag tgccattacc cgttctctgc
      961 gcgactacct gttgccggaa aacagcggcg tgcgtaaagc gttccaggac atggaagcgg
     1021 cgctgcgtga aaaccgcatg acgctggaag cgattcgtgt cacccagtcg gaccgtgacc
     1081 tgtttaagca tctgatcagc gaagctacca actacgtggc ggcggactac atgcgtcacg
     1141 ccaacgagcg ccgtgtccat ctcgacaaag ccctggagtt tcgtcgcgag ctacatactt
     1201 cgcgtcagca actggcggct gagcagtaca aacacgtcga tatggcgcgt gagctggcag
     1261 agcacaacgg tgccgaaggt gatctggaag cggattatca ggcggccagc gatcacctga
     1321 acctggtgca aaccgcattg cgtcagcagg agaagatcga acgctacgaa gtggatctcg
     1381 atgagctgca gatccgtctg gaagagcaaa atgaagtggt ggcagaagcc atcgaacgcc
     1441 aggaagagaa tgaggctcgc gcggaagctg ccgaactgga agtggacgag ctgaaaagcc
     1501 agcttgctga ctaccagcag gcgctggacg tccagcaaac gcgcgcgatc cagtataacc
     1561 aggcgattgc tgcgcttaat cgtgccaaag aactgtgcca tctgccggac ttaaccgccg
     1621 acagcgccgc cgaatggctg gaaaccttcc aggcgaaaga gctggaagcg actgaaaaaa
     1681 tgctctctct tgagcagaaa atgagcatgg cgcaaaccgc gcacagccag tttgagcagg
     1741 cttatcagct ggtggtggca atcaacggcc cgctggcgcg taacgaggcg tgggatgtcg
     1801 ctcgcgaact attgcgcgaa ggggttgatc agcgtcacct ggcagagcag gttcagccgt
     1861 tgcggatgcg cttaagcgaa ctggaacagc gtctgcgtga gcagcaagaa gctgagcgtc
     1921 tgctggcaga tttctgcaaa cgtcagggca agaattttga tattgatgaa ctggaagctc
     1981 tgcatcagga gctggaagca cgcattgcct ctctttccga tagcgtatct aacgcccgtg
     2041 aagagcggat ggcactgcgg caggaacagg aacagctgca gtctcgcatt cagagtttga
     2101 tgcagcgtgc accggtttgg ctggcagcgc aaaacagtct caaccagttg agtgaacagt
     2161 gcggcgaaga gttctcctcc agccaggacg tcactgaata tctgcaacag ttgctggagc
     2221 gtgagcgcga ggcgattgtt gaacgcgatg aagtaggcgc gcgcaaaaac gccgtcgatg
     2281 aagagatcga acgtttaagc cagcctggcg gctctgaaga tcagcgtctg aacgcgctgg
     2341 cggagcgttt tggtggtgtg ctgctgtcag aaatttatga cgacgttagc ctggaagatg
     2401 cgccgtactt ctcggctctg tatggcccgt cacgccacgc catcgtggtg ccagatctgt
     2461 cacaggtaac cgaacacctg gaaggcttga ccgattgccc ggaagatctc tatttgatcg
     2521 aaggggatcc gcagtcattc gatgatagcg tgttcagcgt tgatgagctg gaaaaagcgg
     2581 tggtggtgaa aatcgccgat cgacagtggc gttattcacg tttcccggaa gtgccgctgt
     2641 ttggtcgtgc tgcgcgtgaa agccgtattg aaagcctcca tgccgagcgt gaagtgcttt
     2701 ccgaacgctt cgccacgctc tcctttgatg tacagaaaac ccagcgtctg catcaggcgt
     2761 tcagccgctt tatcggcagt catctggcgg tggcgtttga gtctgacccg gaagcagaaa
     2821 tccgtcaact gaacagccgt cgagtcgaac tggagcgggc gttgagtaat catgaaaatg
     2881 ataaccagca gcagcgtatt cagtttgagc aggcgaaaga aggcgttacg gcgctgaacc
     2941 gcattctgcc gcgtctcaac ctgttggctg atgacagcct ggcggatcgc gtcgatgaaa
     3001 tccgcgaacg tctggatgaa gcccaggaag ccgcgcgttt tgttcagcag tttggcaatc
     3061 aactggcgaa actggaaccg atcgtttcgg tattgcagag cgacccggaa cagttcgaac
     3121 agttaaaaga agattacgcg tactctcagc agatgcagcg cgatgcccgt cagcaagcgt
     3181 ttgccctgac ggaagtggtg cagcgtcgtg cgcactttag ctattctgac tcggcagaaa
     3241 tgcttagcgg taacagcgat ctcaacgaaa aactgcgtga acgtctggaa caggcggaag
     3301 cggagcgtac ccgcgctcgc gaagcgttgc gcggtcacgc agcgcagttg agtcagtaca
     3361 accaggtgct ggcttcgctg aaaagttctt acgacaccaa aaaagagcta ctcaacgatc
     3421 tgcaacgtga attgcaggat atcggcgtgc gtgctgatag cggggcagaa gagcgggcgc
     3481 gtattcgccg tgacgagctg catgcgcaac tgagcaataa ccgttcacgc cgcaatcaac
     3541 tggaaaaagc gcttaccttc tgcgaagcgg agatggacaa cctgacccgc aaactgcgca
     3601 agctggagcg ggattacttt gagatgcgcg agcaggtagt gaccgccaaa gcgggctggt
     3661 gtgcggtgat gcgcatggtg aaagataacg gcgttgagcg ccgcttacac cgtcgtgagc
     3721 tggcttatct ctccgctgat gatttgcgtt ccatgtcgga taaggcgtta ggtgcgctgc
     3781 gtctggcggt ggcggataac gaacatctgc gcgacgtgct gcgcatgtcg gaagatccga
     3841 aacgtccgga gcgtaaaatt cagttcttcg tggcggttta tcagcatctg cgtgaacgta
     3901 ttcgtcagga tattattcgt accgatgatc cggtggaagc tatcgaacag atggagattg
     3961 aacttagccg tctgaccgaa gaattaacct cccgtgaaca gaaactggcg atcagttccc
     4021 gcagcgtggc gaacatcatt cgcaaaacca ttcagcgcga gcagaaccgt atccgtatgc
     4081 tcaaccaggg gttgcagaac gtatcgtttg gtcaggtgaa cagcgtgcgt ctcaacgtga
     4141 acgtgcgtga aacgcacgcc atgctgctgg atgtgctctc tgaacagcac gagcagcatc
     4201 aggatctgtt taacagcaac cgtctgacct tctcggaagc gttggcgaaa ttgtatcagc
     4261 gtcttaatcc gcagattgat atggggcagc gcacgccgca gaccattggc gaagaactgc
     4321 tggattaccg caactatctg gaaatggaag ttgaggttaa ccgtggttcc gatggttggt
     4381 tgcgcgcaga gtctggtgca ttgtcgaccg gtgaggcgat tggtaccggt atgtcgattc
     4441 tggtgatggt ggtacaaagc tgggaagatg aatctcgccg cctgcgcggt aaagatatct
     4501 ctccttgccg cctgctgttc ctcgatgaag cagcgcgact ggatgctcgt tctatcgcca
     4561 cgctgtttga attgtgtgaa cgtttgcaaa tgcagctcat catcgcagcg ccggaaaata
     4621 tcagtccgga aaaaggcacc acctataaac tggtacgtaa agtcttccag aataccgaac
     4681 atgttcacgt cgtcggcctg cgaggatttg cgccgcaact ccctgaaacg cttctaggaa
     4741 gagacgaagc gccttcccag gcgagttaaa attaaggcgg cagcaatgcc gccttttctt
     4801 tttccgaaaa ctccgtttct gcactaaaaa aatggcacat aacg
//
LOCUS       DAJTSI010000219         4779 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000219 DAJTSI010000000
VERSION     DAJTSI010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4779
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.221"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(263..1120)
                     /gene="sitD"
                     /locus_tag="OO327_003830"
     CDS             complement(263..1120)
                     /gene="sitD"
                     /locus_tag="OO327_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR6020189.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1117..1974)
                     /gene="sitC"
                     /locus_tag="OO327_003831"
     CDS             complement(1117..1974)
                     /gene="sitC"
                     /locus_tag="OO327_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR6020190.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1971..2798)
                     /gene="sitB"
                     /locus_tag="OO327_003832"
     CDS             complement(1971..2798)
                     /gene="sitB"
                     /locus_tag="OO327_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR6020191.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2798..3712)
                     /gene="sitA"
                     /locus_tag="OO327_003833"
     CDS             complement(2798..3712)
                     /gene="sitA"
                     /locus_tag="OO327_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR6020192.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4144..>4779)
                     /locus_tag="OO327_003834"
     CDS             complement(4144..>4779)
                     /locus_tag="OO327_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6020193.1"
                     /translation="PLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVD
                     FEKGIVNVRRNLTILDMFGPPKTNAGIRTVALLQPALEALKEQYKLTGHHRKSEITFY
                     HREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHT
                     FACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT         1267 a         1168 c         1127 g         1217 t
ORIGIN      
        1 tgaaggaaga gctgaatagt ctgaaggatt tgaattcggt taggaatttt atcgtggaat
       61 aacagaagct gcggcacgtc gtatgcagga acgtgccgcg gttggctggt aaactttcga
      121 tagtgcgagt attgaatgat ttctaggcgt tatcgatttt acgtattttt tgcatgatag
      181 aattcgtacc tcctcccaac gaccttccat ggctttccgg acttctgtag gctggcggac
      241 gatgctgtcc tcggatgttg tgctaattgg gtgataacag atcctgtgta tgggcgtttc
      301 cctttgtgcg tgcgttgatg ctggtaacgg taaacgtcat gatgaacacg accgcgaata
      361 agacgacaat cgtcggagct ggtgcgctgt cgataaaaaa ggacagccag acaccagtca
      421 ttgacactat taccgatatg ccagtcgcca gtaggagcgc aatatgaaag cgttgtgtca
      481 ggagtactgc aatggcgcca ggcgcaatga gcagagaaat agagagaatg atacctactg
      541 ctttcagcgt cgccacaatg gtcagggaaa ccatgcacag cagcccataa tgcagccatc
      601 tcgtatgcaa accgcttacc tgcgcctgtt gataatcaaa gctgaacagc aaaaaatccc
      661 gccattttac gctaataaca agtgtaacca gcccggcaat aatcaccgtc tggattatgt
      721 cgccgatggt tatcccgagc atgtcgccga aaagaatatg gtcaagatgt acgtctggtt
      781 taattgcaat atacaagatc agacctgcgg caaacatgcc agaaaaaaca atccccataa
      841 ctgtatcttg cttgattcgg ctgttgtctt tcaggtatcc cgtcgccacc gcgcagaata
      901 ctccggcgac gaatgcccct gtagccagag gtaaccccag aatccaggta agcacgatgc
      961 caggaaaaac cgcatgactc atcgcgtcac ccatcagtgc ccaacctttc aggaccagaa
     1021 agaccgacag cagcgcacag ggtatggaaa ctaccagtga aatcagcagt gcgtgactca
     1081 taaaggtgaa ctgcagtggc tccagtaata atgccatcat gagcgatcct ccagcgcttt
     1141 atgcgcacgc cgacgatttg ccaaaagtcc atgggtgggc gcaaatacaa atgctaaaag
     1201 aaacagcagt gtctgcgcca ccacgatgat tccacctgtc gcgccgtcca gaaagtaact
     1261 gacccacgcg ccgaggaaac tggttacact gccaatagtt acagcaatta tgagcaggcg
     1321 gggaaaacga tcggttaaca accatgctgt tgcaccggga gtcaccacca gacagatcac
     1381 cagaaaggca ccaaccgttt gcagcgccgc aacggtcgaa accgacagca gggtgaagaa
     1441 gaggattttt aatctgcccg gatgtaatcc aatagcacgg gcgtgattct cgtcgaaaaa
     1501 ggtcaccata agatctttcc atttgaaaaa aagcacgatg atcgagagaa tgccaatgat
     1561 agtgagttgc aggatatccg ccggatcgat tgccagaata ttgcccagga cgatggtctg
     1621 aatgttcacg gatgtcgggt tcaatgacac cataaacagc cccaggccaa aaaaggacga
     1681 gaagatcagg ccgatgatgg catcttcttt taagcgggtg cgttggttaa gaaacagcat
     1741 gctgcccgct gccagtccgc cggaaaagaa ggctcccaga gaaaacggaa gccccagcat
     1801 atatgccccc gcaacacccg ggacaataga gtgcgacagc gcatcgccaa tcagcgacca
     1861 gccttttagc atcagatagc atgacaggaa agcgcagaga ccgccgacca ttgccgagac
     1921 ccacatcgca ttgagcatat attcatagct gaagggttcc agtagcacgt tcatcttgct
     1981 tccctctgca ccgctgacgg tcgatgtgcc acaaaagggc gttcatcgtc agtaataatg
     2041 ctttcttccg agccgttaag tgtgacatgg cgcagtacgc cgctaaaagc cagctccagg
     2101 ttctcggctg taaaagtggt gtctgtcggc ccgcttgcca ggacggtgcc tttgaccatg
     2161 accgtataat cgcaaaatgt cgtgactgag ccgagattgt gcgttgagac aagcatcgtt
     2221 ttgccttccg cgcgtaactc cctcaacagg ctgatgattt tcgcttcggt tttcacatca
     2281 acgccggtaa aaggttcatc cagcaggatc acatcaccct gctgtgcaat tgctctcgcc
     2341 agaaagacgc gtttcttttg tccaccggaa agctcgccga tttgacgatg gcgaaaatcc
     2401 accatatcga cacgttccag cgcatcggtg acaatctgac gatccctctt tttggctata
     2461 cgcaacatac ccatatgccc atagcggccc atcatcacca catcttccac cagtacagga
     2521 aatgaccagt caacctcttc tgactgaggg acgtaggcaa ccaggttttt ctgtagcgcc
     2581 tgtcgcgtgg gaatacccag aacagatatc tttccgctgg tcagacgcac aaatcccata
     2641 atcgctttaa acaacgtcga ttttccggaa ccgtttaccc caaccagagc ggcaatagat
     2701 ccacttggta ctgtgaagga tgcatcgcgc agtgctgtgt gcccgttacg ccaggtgaca
     2761 gtaacatcat taacgacaat gcctgcagat tgcatcatta tttctccctt ttcccggctt
     2821 taattccctg taccagcgta ctggtagtaa ccttaagaag gtcgatatac gtaggtaccg
     2881 ggccgttttc tgtgctcagg gaatcgacat agagcacacc gccgtagtgt gcgccggttt
     2941 cacgcgcaac ctgacgcgct ggtttatcgg aaatcgtact ctcgctaaag actgccggga
     3001 tatgattttt cttaactata tcaacaacct tacgtacctg ctgcggtgtt ccttgttgat
     3061 cggcattaat cggccacaga taaagctctt ttagccccaa atcgcgtgcg agataagaaa
     3121 aagccccttc actggtgacc atccatcgct gattctcagg gagttccgta atctgcttac
     3181 gcaggggggc aagggtttgg gtaatcttgg ctttataagt atcggcatta cgttggtagg
     3241 tttgtgcatt tgccggatcg tattttatca acgcatcacg aatattatcg acgtaaatca
     3301 gagcattatc tggcgacatc catgcatggg gattaggttt gccctcatag ggtccttcgg
     3361 tgatccctac tggcgtcaca cccgaagaga caattacttc tggaacccca ttgagatgct
     3421 ggtaaaagcg ttggaaccac aattccagat tcataccatt ggcgagaatc agttgtgccc
     3481 cctgcgcacg tttaatatcg ccaggggtag gctgatactc atgaatttct gcaccaggct
     3541 tggttatgga tgagacttct gcagcatctc cagccacgtt tttggccata tccgcgatga
     3601 tggtaaatgt tgtaatgacc ttgaattttt cagttgcact tgcctgaaat gcgatcgagc
     3661 aggtgagggc gaggcacccc aagagcatgg ttactttttt tattgagtgc ataatttagt
     3721 acctatagtg agcaaaatta gaacaaacat agcaacggct atgttttata gcataaccta
     3781 tacaaaatga aaataattat cattaacagc tttgaggcct aaaacaggga ttaggttgtt
     3841 tcctgctggc gacatgatat tgaggaaaaa agtagagcag ctattaggct ttgaaatgac
     3901 tttatgagga aaattgttga tggcaaaaat ggggcaaaaa agagtgggtt atcgtagctt
     3961 attgttgtcg ctgataatgt ttaacatatt gaaaaataaa taaaatactt atgagtcaga
     4021 tagttgtgat ttttgccctt acttgttcag gttgtattgt tctttctttg taatttgttg
     4081 attttcttgc attatttcag ttctctggta ctaaatgggg caaattgggg gcaaactttg
     4141 caactacgat aaccgcgcat tcaacatggc tatctgttcg tcgttcatgt catcaatcca
     4201 cataccgtaa atttcataca ccatttgcgc agtttcatgc cccatctggc tggctataaa
     4261 tgccgggttc gctcctgccg tcaacagcca gcaggcaaaa gtatgtcgcg tatggtacgg
     4321 attacggcgg cgaataccag cacgttttac tgctgcattc catcttgtac ccaaactgct
     4381 taccgagtaa taaggtttct gtttttcgtt acacaccctg ggcatgaaaa caaaatgcag
     4441 tttttgtttt tcagttctgc cgtactcccg atgataaaaa gtgatttcgc ttttgcgatg
     4501 atgcccggtc agtttgtatt gctccttcag tgcttcaaga gccggctgca gtaatgctac
     4561 cgtccggatc ccggcatttg tttttggggg accgaacata tcaagtatcg tcaggtttct
     4621 tctgacattc acaattccct tctcgaaatc cacatcctcc cacgccagag ctgccagttc
     4681 cccgtgacga agcccggagt aaacggcaaa tttccacaag ttttggctct gtcctttttc
     4741 actttccatt aatgcattga attctgtttt agataacgg
//
LOCUS       DAJTSI010000220         4732 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000220 DAJTSI010000000
VERSION     DAJTSI010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4732
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.222"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            76..260
                     /locus_tag="OO327_003835"
                     /pseudo
     CDS             76..260
                     /locus_tag="OO327_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            403..624
                     /locus_tag="OO327_003836"
                     /pseudo
     CDS             403..624
                     /locus_tag="OO327_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(645..707)
                     /locus_tag="OO327_003837"
                     /pseudo
     CDS             complement(645..707)
                     /locus_tag="OO327_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1157..2569
                     /gene="ospC1"
                     /locus_tag="OO327_003838"
     CDS             1157..2569
                     /gene="ospC1"
                     /locus_tag="OO327_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCR6020194.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKSFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2673..2822)
                     /locus_tag="OO327_003839"
                     /pseudo
     CDS             complement(2673..2822)
                     /locus_tag="OO327_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2898..4595
                     /gene="ospD3"
                     /locus_tag="OO327_003840"
     CDS             2898..4595
                     /gene="ospD3"
                     /locus_tag="OO327_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCR6020195.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMEIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         1648 a          852 c          879 g         1353 t
ORIGIN      
        1 acaatgacat tgcctgtttt tataacggta attgcagacc atgacaaacc gcagccgtca
       61 ggctgcctac tcgagtcaca aggctctttg tgtccgatat gccggcagcg cattacccat
      121 gaaactggct ggaacgtaca ccataaggtg aagaaagtta tgggggcggt gaagaattat
      181 ctaaccttgt cttgttacat ccaaattgcc atcgacagtt acacagttgt gaagccggct
      241 ctctcaaaaa gggcttataa aggcttgagc ggtgtaccgg gaaaccggta tgcaccgttc
      301 ttaggggagg gcagtccagc aatgaactgt ccttacccga caaacatcca aaacgaacgg
      361 aacgtgctgg aatccgcata taacccgttg taaagtgagg aaatggacac ctcacttgct
      421 catgagaacg cccgcctgcg ggcactgttg cagacgcaac aggacactat ccaccagatg
      481 gctgaataca accgcctgct ctcacagcgg atggcggctt atgcttccga aatcaaccga
      541 ctgaaggcgc tggttgcgaa actgcagcgt atgcagttcg gtaaaagctc agaaaaactt
      601 cgcgcaaaaa ccgaacggca gattccattt tcacgggcga tctacgccac acaggctctg
      661 ggttgcagtg actgctccat taaggcgatt ttgaactcat aagggaagta acaaaaaaac
      721 tgcaaaagta caataattat atatggccaa actaacctct attaaaaaac atcagttttt
      781 cacacttagt tgagcttcat gaaaaaatca tcgaggattt cattgcagaa tacacattcc
      841 actaactgga agcactccat aagaggaatc agagaaagta tcgttttttt atagtaaaat
      901 tcattgctgt tcaataaaat tgatatagtt attaaattat gaaaataatt tcacaaaaaa
      961 gattaacttg agttcttaag catcggattt tagatatttt gaaatatctg gcatcacata
     1021 acatcaaaag tatataatac tcttatctaa ttacatgcag tatattgtat atatactcaa
     1081 aaattgtaat tcataccctg ctatatcttg tagggaaaga tattaattaa aactgttttc
     1141 atataaggtt cattttatga atatatcaga aacactgaac tcagcaaata cccaatgcaa
     1201 tatagattct atggataaca gattacatac attgtttcca aaagtgacat cagtgcgaaa
     1261 cgctgcacaa caaactatgc cagatgaaaa aaatttaaaa gatagtgcaa atattattaa
     1321 aagtttcttt aggaaaacta tagcagcaca gagttatagt agaatgttct ctcaaggctc
     1381 taactttaaa tctttaaata tagcaattga tgcaccatca gacgctaaag cctcatttaa
     1441 ggctattgag caccttgaca gattatcgaa gcattatata tctgaaataa gggaaaaact
     1501 tcatcctctt tctgcagagg aactcaattt gctttcgcta attattaatt ctgatttaat
     1561 cttcagacat caaagtaatt ctgatttgtc tgataaaatt ttaaacatta agtcattcaa
     1621 taaaattcag tctgaaggaa tatgcacaaa acgaaacaca tacgctgatg atataaaaaa
     1681 aatagctaat catgactttg tgttttttgg cgttgaaatc tctaaccatc agaaaaaaca
     1741 ccccctgaat acaaaacatc acactgttga ttttggtgca aatgcgtata tcattgatca
     1801 tgactctcca tatggatata tgacattaac cgatcacttt gataatgcta ttccacctgt
     1861 tttttaccat gagcaccaat catttttaga taaattttca gaggttaata aagaagttag
     1921 tcgatacgta catggaagta aaggaattat agatgtacca atattcaata ctaaagatat
     1981 gaagttaggg ctcggattat acctgattga ctttattaga aaaagtgaag accaaagctt
     2041 caaggagttt tgctatggaa aaaatcttgc ccctgtggat ctggatagaa tcataaactt
     2101 tgtttttcag ccagagtacc atatacctag gatggtaagt acagaaaact tcaaaaaagt
     2161 taagattaga gaaatatcct tagaggaggc tgttacagca tctaattacg aagaaattaa
     2221 caagcaggtc actaacaaaa aaattgctct ccaggctctt tttctttcga ttactaatca
     2281 aaaagaggat gtcgccttat atatattatc taattttgag ataactagac aagatgttat
     2341 ttccataaag catgagttgt atgatattga gtatctactt agcgctcata attcaagctg
     2401 taaagtactt gagtatttta tcaataaggg attggttgat gtaaacacaa agttcaaaaa
     2461 aactaatagt ggggattgta tgttggataa cgcaataaaa tatgagaatg cagaaatgat
     2521 aaaactatta ttgaaatatg gtgcaacatc tgacaataaa tatatttaat caaaattgaa
     2581 tatcgtttag caaaaggcag tatttgcaca aaatatcgat atattgttta ttgtcagtat
     2641 ggctcaatgt gataatagtt ggaaagtttg atgggtttcg ccccgttgta gcggtagtcg
     2701 aactggaagg tcttcaggca ctgcttacag cgatagagca gcccccctga actggaatgg
     2761 ccgttccgga tacccccctg agtttcagag taacggtgac aaaccacatc aatctttgcc
     2821 atcaatcatc caaagggcaa agagtacaac aacactaagt ctgcgtcaca acccatcaat
     2881 gaaaggaata tatacatatg ccatcagtaa atttaatccc atcaaggaaa atatgtttgc
     2941 aaaatatgat aaataaagac aacgtctctg ttgagacaat ccagtctcta ttgcactcaa
     3001 aacaattgcc atatttttct gacaagagga gttttttatt aaatctaaat tgccaagtta
     3061 ccgatcactc tggaagactt attgtctgtc gacatttagc ttcctactgg atagcacagt
     3121 ttaacaaaag tagtggtcac gtggattatc atcactttgc ttttccggat gaaattaaaa
     3181 attatgtttc agtgagtgaa gaagaaaagg ctattaatgt gcctgctatt atttattttg
     3241 ttgaaaacgg ttcatgggga gatattattt tttatatttt caatgaaatg atttttcatt
     3301 ccgaaaaaag cagagcacta gaaataagta catcaaatca caatatggca ttaggcttga
     3361 agattaaaga aactaaaaat gggggggatt ttgtcattca gctttatgat cccaaccata
     3421 cagcaactca tttacgagca gagtttaaca aatttaactt agctaaaata aaaaaactga
     3481 ctgtagataa ttttcttgat gaaaaacatc agaaatgtta tggtcttata tccgacggta
     3541 tgtctatatt tgtggacaga catactccaa caagcatgtc ctccataatc agatggcctg
     3601 ataatttact tcaccccaaa gttatttatc acgcgatgcg tatgggattg actgagctaa
     3661 tccaaaaagt aacaagagtc gtacaactat ctgacctttc agacaatacg ttagaattac
     3721 ttttggcagc caaaaatgac gatggtttgt caggattgct tttagcttta caaaatgggc
     3781 attcagatac aatcttagca tacggagaac tcttggaaac ttctggactt aaccttgata
     3841 aaacggtaga actactaact gcggaaggaa tgggaggacg aatatcgggt ttatcccaag
     3901 cacttcaaaa tgggcatgca gaaactatca aaacatacgg aaggcttctc aagaagagag
     3961 caataaatat cgaatacaat aagctgaaaa atttgctgac cgcttattat tatgatgaag
     4021 tacacagaca gatacccgga ctaatgtttg ctcttcaaaa tggacatgca gatgctatac
     4081 gcgcatacgg tgagctcatt cttagccccc ctctcctcaa ctcagaggat attgtaaatt
     4141 tgctggcctc aaggagatat gacaatgttc ccggacttct gttagcattg aataatggac
     4201 aggctgatgc aatcttagct tatggtgata tcttgaatga ggcaaaactt aacttggata
     4261 aaaaagcaga gctgttagaa gcgaaagatt ctaatggttt atctggattg tttgtagcct
     4321 tgcataatgg atgtgtagaa acaattattg cttatgggaa aatacttcac actgcagacc
     4381 ttactccaca tcaggcatca aaattactgg cagcagaagg cccaaatggg gtatctggat
     4441 taattatagc ttttcaaaat aggaattttg aggcaataaa aacttatatg gaaataataa
     4501 aaaatgaaaa tattacacct gaagaaatag cagaacactt ggacaaaaaa aatggaagtg
     4561 attttctaga aattatgaag aatataaaaa gctgaatatt atctcgtggt gatacactga
     4621 atttgaggta accggctcat ttaaaccgtc tggtctgtta cctccggttc cacaaaaata
     4681 atgtccatca ttttgaagtg gacactatcg tatggaatac cggacctgga tc
//
LOCUS       DAJTSI010000221         4731 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000221 DAJTSI010000000
VERSION     DAJTSI010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.223"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1100)
                     /locus_tag="OO327_003841"
     CDS             complement(<1..1100)
                     /locus_tag="OO327_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HCR6020196.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGA"
     gene            complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="OO327_003842"
     CDS             complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="OO327_003842"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR6020197.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="OO327_003843"
     CDS             complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="OO327_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR6020198.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="OO327_003844"
     CDS             complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="OO327_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR6020199.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="OO327_003845"
     CDS             complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="OO327_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR6020200.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="OO327_003846"
     CDS             complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="OO327_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR6020201.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3404..4381
                     /gene="pspF"
                     /locus_tag="OO327_003847"
     CDS             3404..4381
                     /gene="pspF"
                     /locus_tag="OO327_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR6020202.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4501..>4731)
                     /locus_tag="OO327_003848"
     CDS             complement(4501..>4731)
                     /locus_tag="OO327_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6020203.1"
                     /translation="AVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCG
                     AYGNVIRFLYPLTIPDAQFDAAMKILQDALSD"
BASE COUNT         1179 a         1194 c         1120 g         1238 t
ORIGIN      
        1 aatgcacctt cctgctgtaa tgcctcgacc agcgctaata tttcgctttc tggaagcaag
       61 ccacgcagtg ggttcaaccc gatgccatcg tgagaggcga ggaagttaaa ccaggtcgtt
      121 ttactggagg gcaagctcag actttgcgcc cactggcaca gcgcctcgac gttctgtttt
      181 tgcaccgcat gtaacaccag cggcggcagc gagaactggt acaccatatg cgcttcgtca
      241 tcaccgttgc caaagtaagc aatgttgtct ttgtgcggaa cattggtttc ggtaataatc
      301 actgtacctg gtgctacgtc atcaataatc gaccgtaaca gcttgataat cagatgtgtt
      361 ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac ggcatccagg
      421 cggacatact cagcaccttt ctcaaggtaa cagagtaaaa catccaccat cgccagcaac
      481 acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt ccataaatgg
      541 cgcgtggaat tatcatccat ttgaaacggc gttaatagcg gtaacgcacg cggaagggtg
      601 acggcactga gatcggtttt cggatcaacc gcaataaaga aatcttcaaa acccggaagc
      661 tgttgtaaat agtttttaaa ccattcactt tttgcagaca tatggttgca gacaaaatca
      721 aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc cgcttcactg
      781 gcgacctgat gataatcaat taccgaaaat ccatcatcag atgaccatgg gtaaaaaggt
      841 aataaatgaa catgtgaaaa aatgctttgc agccattgat ggtagaactg attaaatatg
      901 ggtagtggtt ttaaatcatt actgtgaaat tgatcggcat aggtaattaa cacgacatca
      961 ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact ctcaagacgc
     1021 gttacaagtt tctgtaaatg agttgcggta aatgttccac cgtagatttc gtccatgtaa
     1081 tccgtaattt tctgtttcat gttgcctttc cgtggtagcg gtcccatgag gcgagagact
     1141 actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca ggaaatcatt
     1201 ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatagg gatgtaaata
     1261 aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct tttaaccttt
     1321 gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg tatatcccat
     1381 ctcgctaagg agctcttttg cctgcccgga ctggcgtccg gcattgcaat acactttcac
     1441 ggtgtcgttt ttatccggaa cggcggtggc aatgcgctct ttcacttcct tcaggggaat
     1501 attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa catcgatcca
     1561 gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta agcctttttt
     1621 taaacatgta agcgtccttc tatggatgaa aattgccatc cattatacgc ctgagattta
     1681 acaatttttg cgaaactccc ctcacctttt ataacgctgt gccagtttat tagcagcccg
     1741 acttaacagc ggttccagcg ccacagccag caacattttc agcggtcggc gagcaactga
     1801 ttttatcgcc cagcccgcca cacccgccgg gccatagcgc agtgcggtaa gaagtaccag
     1861 cttgcctgct aatttgaaac caagcttcac cttttgcccg gcctgttgcc agcgagtatt
     1921 cataactttc ctcacagttg acggaaacgg ctacgtaacg tgaaagtatc ggaagtgaca
     1981 taacgttcca tctcgcgtaa acgcgtttca cttgccgcca gttcacggtc gacttcatcc
     2041 agcaattcgc tgctggaagg tagctgctca ccaaaggcca tgttgtccgg cattggatca
     2101 agcgcaaatg acaaaatgat gtaagcaacc agggtaaaca gcgccagacc gaaaaaaatc
     2161 gacagcacca ccaggatacg caccagtttt accggtacat caaaatagtt ggcaatcccg
     2221 gcgcagacgc cgcggaccat gccctgctgt ggaatacgcc ataacttttt attgagatta
     2281 atgcccgcca ttagcgatcc ctccagttcg gatgttctgc atcaagaata gattccagcg
     2341 cctgaatacg ttcgcgcatc cgttttgctt catcaaccag ttgcgctaat cgctgctgct
     2401 cactttgcga caattcactg cgaccagaac gattgctgta atgcagccat aaccagatcg
     2461 gtaaaacaaa cagcacaaaa atggttaacg gaatagccag aaatagcgcg ctcataagta
     2521 ctccttagac gatgagcggc ggcgcgttcg gacgccgcct ggatgttatt attgattgtc
     2581 ttgcttcatt ttggctttta attgtgccag ttgttcgctg attgcatcat cggctttcag
     2641 ttcggcaaac tgatcgtcca gcgatttttg tttaccgaag ctgtggcttt ctgcttccgc
     2701 ttccatctgg tcaatacgac gttcgaaaga ttcgaaacga gccattgctt catccagttt
     2761 gccactgtcc agctgacgac gcacatcgcg cgacgagttt gccgcctgat gacgtaacat
     2821 caatgcctgc tggcgagcgc gtgtttcgct caatttgttt tccagctcgc caatctcttt
     2881 cttcatgcgt gccagcgtat cgtccaccag cgtcacttca tgttccaggg acttaatcag
     2941 atcggtcagt ttctgttttt caattaacgc tgcacgtgcc agatcctctc tctctttcag
     3001 cagcgccagt tcggcttttt cctgccattc aacttcacgc gccgacgctt gttcaatacg
     3061 gcgagtcagc tgtttctttt ctgccaacgc acgcgccgaa gtagaacgta cttcaaccag
     3121 tgtatcttcc atctcctgga tcatcagacg caccagtttc tgtggatctt ccgctttctc
     3181 taacagagcg ttgatgttgg cattcacgat gtcggcaaag cgagaaaaaa tacccataat
     3241 gttgtcctct tgatttctgc gttcaggatt gtcctgctga actgttaata caatttgcgt
     3301 gccaattttt tatctttttg atttataaag atctgattga agaatcaaca gcaacatgcc
     3361 aggatgaatt agcgaattac actaacaagt ggcgaatttc atcatggccg aatacaaaga
     3421 taatttactt ggtgaggcga acagctttct cgaagtgctg gaacaggttt cgcatctcgc
     3481 accgctggac aaaccggtgc tcatcatcgg cgaacgcggc accggtaaag agttgattgc
     3541 cagccgcctg cattatctct cctcccgttg gcaagggccg tttatttccc ttaactgcgc
     3601 ggcgttaaat gaaaatctgc tggattccga actgtttggt cacgaagcgg gggcgtttac
     3661 cggtgcgcaa aaacgtcatc ctgggagatt tgaacgtgcc gacggcggta cgctatttct
     3721 tgatgaactc gctacggcac cgatgatggt gcaggaaaaa ttattgcgtg tgattgagta
     3781 cggtgaactg gagcgcgttg gcggtagcca gccgttgcag gtgaatgtgc ggttggtatg
     3841 cgcgacgaat gccgatctcc cggcgatggt caatgaaggt acttttcgcg ctgacctgct
     3901 cgaccggctg gctttcgatg tagtgcaact gccgcccctg cgcgagcgcg aaagcgacat
     3961 tatgctgatg gcagaacact ttgccattca gatgtgtcgg gaaatcaagc tgcctctgtt
     4021 cccggggttt acggagcgcg ccagagaaac attgctgaat tatcgttggc cgggaaatat
     4081 tcgtgaattg aaaaacgtgg tggaacgttc agtgtatcgc cacggcacca gcgattatcc
     4141 gcttgatgac atcattattg atccctttaa acggcgtcca cctgaagaag ccatcgccgt
     4201 ttcagaaaat acctcgcttc cgacgctgcc gctggattta cgcgagtttc agatgcagca
     4261 ggaaaaagag ttgctgcaac tcagtttgca acaggggaaa tataaccaga agcgcgcggc
     4321 tgaattactg gggttaactt atcatcagtt ccgcgcgttg ttgaaaaagc accagattta
     4381 gcgaagttga gcaatacaac ttgccggatg cggcgtgaat gctttattcg gcatacataa
     4441 tcaatacgtc gtttgcaggc ctgataagcg cagcgcatca gacattattg cgttgggcta
     4501 ttaatcgctc agcgcatcct gcaaaatttt cattgccgca tcgaattgcg catccgggat
     4561 ggtcagcgga tacaggaagc gaatcacgtt gccgtatgcg ccacaggtca gcaggagcag
     4621 cccctgcgcc agcgcgcgtt gctggatttt ctgtgcaatc gccgctgacg gctcgcccgt
     4681 ttgcggatcg ttaaactctg ccgcaatcat cgaccccagg ccgcgtaccg c
//
LOCUS       DAJTSI010000222         4724 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000222 DAJTSI010000000
VERSION     DAJTSI010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.224"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..150
                     /locus_tag="OO327_003849"
     CDS             <1..150
                     /locus_tag="OO327_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uL15 family ribosomal protein"
                     /protein_id="HCR6020204.1"
                     /translation="KAANIIGIQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAG
                     GKIEE"
     gene            158..1489
                     /gene="secY"
                     /locus_tag="OO327_003850"
     CDS             158..1489
                     /gene="secY"
                     /locus_tag="OO327_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR6020205.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            1521..1637
                     /gene="rpmJ"
                     /locus_tag="OO327_003851"
     CDS             1521..1637
                     /gene="rpmJ"
                     /locus_tag="OO327_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR6020206.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            1784..2140
                     /gene="rpsM"
                     /locus_tag="OO327_003852"
     CDS             1784..2140
                     /gene="rpsM"
                     /locus_tag="OO327_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR6020207.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            2157..2546
                     /gene="rpsK"
                     /locus_tag="OO327_003853"
     CDS             2157..2546
                     /gene="rpsK"
                     /locus_tag="OO327_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR6020208.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            2580..3200
                     /gene="rpsD"
                     /locus_tag="OO327_003854"
     CDS             2580..3200
                     /gene="rpsD"
                     /locus_tag="OO327_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR6020209.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            3226..4215
                     /gene="rpoA"
                     /locus_tag="OO327_003855"
     CDS             3226..4215
                     /gene="rpoA"
                     /locus_tag="OO327_003855"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR6020210.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            4256..4639
                     /gene="rplQ"
                     /locus_tag="OO327_003856"
     CDS             4256..4639
                     /gene="rplQ"
                     /locus_tag="OO327_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR6020211.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         1136 a         1111 c         1267 g         1210 t
ORIGIN      
        1 aaagcggcta acattatcgg tatccagatc gagttcgcga aagtgatcct ggctggcgaa
       61 gtaacgactc cggtaactgt tcgtggcctg cgtgttacta aaggcgctcg tgctgctatc
      121 gaagctgctg gcggtaaaat cgaggaataa gtagcagatg gctaaacaac cgggattaga
      181 ttttcaaagt gccaaaggtg gcttaggcga gctgaaacgc agactgctgt ttgttatcgg
      241 tgcgctgatt gtgttccgta ttggctcttt tattccgatc cctggtattg atgccgctgt
      301 acttgccaaa ctgcttgagc aacagcgagg caccatcatt gagatgttta acatgttctc
      361 tggtggtgct ctcagccgtg cttctatctt tgctctgggg atcatgccgt atatttcggc
      421 gtcgatcatt atccagctgc tgacggtggt tcacccaacg ttggcagaaa ttaagaaaga
      481 aggggagtct ggtcgtcgta agatcagcca gtacacccgt tacggtactc tggtgctggc
      541 aatattccag tcgatcggta ttgctaccgg tctgccgaat atgcctggta tgcaaggcct
      601 ggtgattaac ccgggctttg cattctactt caccgctgtt gtaagtctgg tcacaggaac
      661 gatgttcctg atgtggttgg gcgaacagat cactgaacga ggtatcggca acggtatttc
      721 aatcattatc ttcgccggta ttgtcgcggg actcccgcca gccattgccc atactatcga
      781 gcaagcgcgt caaggcgacc tgcacttcct cgtgttgctg ttggttgcag tattagtatt
      841 tgcagtgacg ttctttgttg tgtttgttga gcgtggtcaa cgccgcattg tggtaaacta
      901 cgcgaaacgt cagcaaggtc gtcgtgtcta tgctgcacag agcacacatt taccgctgaa
      961 agtgaatatg gcgggggtaa tcccggcaat cttcgcttcc agtattattc tgttcccggc
     1021 gaccatcgcg tcatggttcg ggggcggtac tggttggaac tggctgacaa caatttcgct
     1081 gtatttgcag cctgggcaac cgctttatgt gttactctat gcgtctgcaa tcatcttctt
     1141 ctgtttcttc tacacggcgt tggttttcaa cccgcgtgaa acagcagata acctgaagaa
     1201 gtccggtgca tttgtaccag gaattcgtcc gggagagcaa acggcgaagt atatcgataa
     1261 agtaatgacc cgcctgacct tggttggtgc gctgtatatt acctttatct gcctgatccc
     1321 ggagttcatg cgtgatgcaa tgaaagtacc gttctacttc ggtgggacct cactgcttat
     1381 cgttgttgtc gtgattatgg actttatggc tcaagtgcaa actctgatga tgtccagtca
     1441 gtatgagtct gcattgaaga aggcgaacct gaaaggctac ggccgataat tggtcgcccg
     1501 agaagttacg gagagtaaaa atgaaagttc gtgcttccgt caagaaatta tgccgtaact
     1561 gcaaaatcgt taagcgtgat ggtgtcatcc gtgtgatttg cagtgccgag ccgaagcata
     1621 aacagcgcca aggctgattt tttcgcatat ttttcttgca aagttgggtt gagctggcta
     1681 gattagccag ccaatctttt gtatgtctgt acgtttccat ttgagtatcc tgaaaacggg
     1741 cttttcagca tggaacgtac atattaaata gtaggagtgc atagtggccc gtatagcagg
     1801 cattaacatt cctgatcata agcatgccgt aatcgcatta acttcgattt atggcgtcgg
     1861 caagacccgt tctaaagcca tcctggctgc agcgggtatc gctgaagatg ttaagatcag
     1921 tgagctgtct gaaggacaaa tcgacacgct gcgtgacgaa gttgccaaat ttgtcgttga
     1981 aggtgatctg cgccgtgaaa tcagcatgag catcaagcgc ctgatggatc ttggttgcta
     2041 tcgcggtttg cgtcatcgtc gtggtctccc ggttcgcggt cagcgtacca agaccaacgc
     2101 acgtacccgt aagggtccgc gcaaaccgat caagaaataa tcggggtgat tgaataatgg
     2161 caaaggcacc aattcgtgca cgtaaacgtg taagaaaaca agtctctgac ggcgtggctc
     2221 atatccatgc ttctttcaac aacaccatcg tgactatcac tgatcgtcag ggtaacgcgt
     2281 tgggttgggc aacagccggt ggttccggtt tccgtggttc tcgcaaatcc actccgtttg
     2341 cagctcaggt tgcagcagag cgttgcgctg acgccgtgaa agaatacggc atcaagaatc
     2401 tggaagttat ggttaaaggt ccgggtccag gccgcgaatc tactattcgt gctctgaacg
     2461 ccgcaggttt ccgcatcact aacattactg atgtgactcc gatccctcat aacggttgtc
     2521 gtccgccgaa aaaacgtcgc gtataacgcc tcgttttcca ggtttgttgg agaaagaaaa
     2581 tggcaagata tttgggtcct aagctcaagc tgagccgtcg tgagggcacc gacttattcc
     2641 ttaagtctgg cgttcgcgcg atcgatacca agtgtaaaat tgaacaagct cctggccagc
     2701 acggtgcgcg taaaccgcgt ctgtctgact atggtgtgca gttgcgtgaa aagcaaaaag
     2761 ttcgccgtat ctatggtgtg ctggagcgtc agttccgtaa ctactacaaa gaagcagcac
     2821 gtctgaaagg caacaccggt gaaaacctgt tggctctgct ggaaggtcgt ctggacaacg
     2881 ttgtataccg tatgggcttc ggtgccactc gtgcagaagc acgtcagctg gttagccata
     2941 aagcaattat ggtaaacggt cgtgttgtta acatcgcttc ttatcaggtt agtccgaatg
     3001 acgttgtaag cattcgtgag aaagcgaaga agcagtctcg cgtgaaagcc gctctggagc
     3061 tggctgagca gcgtgaaaag ccaacttggc tggaagttga tgctggcaag atggaaggta
     3121 cgtttaagcg taagccggag cgttctgatc tgtctgcgga cattaacgaa cacctgatcg
     3181 tcgagcttta ctccaagtaa agcttagtac caaagagagg acacaatgca gggttctgtg
     3241 acagagtttc taaaaccgcg cctggttgat atcgagcaag tgagttcgac gcacgccaag
     3301 gtgacccttg agcctttaga gcgtggcttt ggccatactc tgggtaacgc actgcgccgt
     3361 attctgctct catcgatgcc gggttgcgcg gtgaccgagg ttgagattga tggtgtacta
     3421 catgagtaca gcaccaaaga aggcgttcag gaagatatcc tggaaatcct gctcaacctg
     3481 aaagggctgg cggtgagagt tcagggcaaa gatgaagtta ttcttacctt gaataaatct
     3541 ggcattggcc ctgtgactgc agccgatatc acccacgacg gtgatgttga aatcgtcaag
     3601 ccgcagcacg tgatctgcca cctgaccgat gagaacgcgt ctattagcat gcgtatcaaa
     3661 gttcagcgcg gtcgtggtta tgtgccggct tctacccgaa ttcattcgga agaagatgag
     3721 cgcccaatcg gccgtctgct ggtcgacgca tgctacagcc ctgtggagcg tattgcctac
     3781 aatgttgaag cagcgcgtgt agaacagcgt accgacctgg acaagctggt catcgaaatg
     3841 gaaaccaacg gcacaatcga tcctgaagag gcgattcgtc gtgcggcaac cattctggct
     3901 gaacaactgg aagctttcgt tgacttacgt gatgtacgtc agcctgaagt gaaagaagag
     3961 aaaccagagt tcgatccgat cctgctgcgc cctgttgacg atctggaatt gactgtccgc
     4021 tctgctaact gccttaaagc agaagctatc cactatatcg gtgatctggt acagcgtacc
     4081 gaggttgagc tccttaaaac gcctaacctt ggtaaaaaat ctcttactga gattaaagac
     4141 gtgctggctt cccgtggact gtctctgggc atgcgcttgg aaaactggcc accggcaagc
     4201 atcgctgacg agtaaccgga tcacaggtta aggttttact gagaaggata aggtcatgcg
     4261 ccatcgtaag agtggtcgtc aactgaaccg caacagcagc catcgccagg ctatgttccg
     4321 caatatggca ggttcactgg ttcgtcatga aatcatcaag acgactctgc ctaaagcgaa
     4381 agagctgcgc cgcgtagttg agccgctgat tactcttgcc aagactgata gcgttgctaa
     4441 tcgtcgtctg gcattcgccc gtactcgtga taacgagatc gtggcaaaac tgtttaacga
     4501 actgggcccg cgtttcgcga gccgtgccgg tggttacact cgtattctga agtgtggctt
     4561 ccgtgcaggc gacaacgcgc cgatggctta catcgagctg gttgatcgtt cagagaaagc
     4621 agaagctgct gcagagtaat ctgaagcaat gtaaaaaaac ccgccccggc gggttttttt
     4681 atacccgtag tatccccact tatctacaat agctgtactc tttt
//
LOCUS       DAJTSI010000223         4643 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000223 DAJTSI010000000
VERSION     DAJTSI010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.225"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..835)
                     /locus_tag="OO327_003857"
     CDS             complement(<1..835)
                     /locus_tag="OO327_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HCR6020212.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     EQ"
     gene            1017..2441
                     /gene="sbcB"
                     /locus_tag="OO327_003858"
     CDS             1017..2441
                     /gene="sbcB"
                     /locus_tag="OO327_003858"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR6020213.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(2484..2711)
                     /locus_tag="OO327_003859"
     CDS             complement(2484..2711)
                     /locus_tag="OO327_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR6020214.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(2725..3783)
                     /gene="yeeE"
                     /locus_tag="OO327_003860"
     CDS             complement(2725..3783)
                     /gene="yeeE"
                     /locus_tag="OO327_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR6020215.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3962..>4643)
                     /gene="plaP"
                     /locus_tag="OO327_003861"
     CDS             complement(3962..>4643)
                     /gene="plaP"
                     /locus_tag="OO327_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR6020216.1"
                     /translation="TKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPD
                     ASQPEIMLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGY
                     VHPKWRTPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREK
                     RNKTLKDHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNP
                     VPQYEDVA"
BASE COUNT         1256 a         1205 c         1099 g         1083 t
ORIGIN      
        1 tttgttcgaa gattggacgg cggataacat tacccatctt gctatgctct ttcggtacgg
       61 tccatacgtt gtccagcagg tcaaattctg tctttgttgc cagcctaagc tctgagagcc
      121 tcgcccccca cagcataagc atctgatgaa ggagcttatt tgacgtagac gcacggcttc
      181 tttcaatagc aagccaaatc ttagccagtt cgtgatacga cagtacccga tcccctacct
      241 cagcgcggga gccgaagtcc cttggctgga tgctcataat tgcgcaacta tctatcaact
      301 gacgccgcat gcaccaacta attgctgatc ttagttgact tagcacctgc cttgctcggc
      361 gtggattatc tttttcttct tcggtaagca ggtcaaccca ttgcttaacc gtgatagaag
      421 atgccggacg attagggaag gcgtcatgca tgcgcttcat aaccgttgat cgataaagtg
      481 cctgggtctt ttctctgaga gttgtagaga cgtagttgtc gaaccagtag tcgagacact
      541 gggcgaccgt catggagttc tccaccttct cttcaaaata ggtgcgtgga tccgttcctg
      601 agaaatagag ctttcgcaag tcagcagtga tctgtctggc atccttcaaa gacagggatg
      661 ggtatcgacc aagcccaagt cgattaggct tgccatgcca gcgatagcgg tactggaact
      721 ggatgacccc cttcggtgaa attcgtacgc tgaggccatc ggcatcagcc acttcttgtg
      781 ggcccgaata tggtttacca taaatagtac gcagttttgt gtcgcttata gccataaata
      841 atatttggta cgctcagaaa taatagtttg gcacacatct ggtacacaat atcacatgga
      901 caacagcgca cagtaaacaa ctatatttga cgttcgtaga catactcagg tgaaaaaagg
      961 gttgcttttc gagttataac agacagttaa ttaactacag tggacaatgc tagacaatga
     1021 cagacacaga taaacaacct accttcctct ttcacgatta cgaaaccttt ggcacgcacc
     1081 ccgcgttaga tcgccctgca cagttcgcag ccattcgcac cgatgacgaa ttcaatgtca
     1141 tcggcgaacc cgaagtcttt tactgcaagc ccgcggatga ctatttaccc cggcctggag
     1201 cagtattaat taccggtatt accccgcagg aggcacgggc gaaaggagaa aacgaagccg
     1261 cgtttgccgc ccgtattcac tcgcttttta ccgtaccgaa gacctgtatt ctgggctaca
     1321 acaatgtgcg tttcgacgac gaagtcacac gcaacgtttt ttatcgtaat ttctacgatc
     1381 cttacgcctg gagctggcag catgataact cgcgctggga tttactggat gttatgcgtg
     1441 cctgttatgc cctgcgcccg gaaggaataa actggcctga aaatgatgac ggtctaccga
     1501 gctttcgcct tgagcattta accaaagcga atggtattga acatagcaac gcccacgatg
     1561 cgatggctga tgtgtacgcc actattgcga tggcgaaact ggtaaaaacg cgtcagccac
     1621 gcctgtttga ttatctcttt acccatcgta ataaacacaa actgatggcg ttgattgatg
     1681 ttccgcagat gaaacccctg gctcacgttt ccggaatgtt tggggcatgg cgtggcaata
     1741 ccagctgggt ggcaccgctg gcgtggcatc ctgaaaatcg caatgccgta attatggcgg
     1801 atttggcagg agacatttcg ccattactgg agctggatat cgacacattg cgcgagcgtt
     1861 tatataccgc aaaagccgat cttggcgata acgccgccgt tccggttaag ctggtgcata
     1921 tcaataaatg tccggtactg gcccaggcga atacgctacg cccggaagat gccgaccgac
     1981 tgggaattaa tcgtcagcat tgcctcgata acctaaaaat tctgcgtgaa aatccgcaag
     2041 tgcgtgaaaa agtggtagcg atattcgcgg aagccgaacc gtttacacct tcagataatg
     2101 tggatgcaca gctttataat ggctttttca gtgacgccga tcgtgcagca atgaaaattg
     2161 tgctggaaac cgagccgcgt aatttaccgg cactggatat cacttttgtt gataaacgaa
     2221 ttgaaaagtt gttgttcaat tatcgggcac gcaacttccc ggggacgctg gattatgccg
     2281 agcagcaacg ctggctggag caccgccacc aggtcttcac gccagagttt ttgcagggtt
     2341 atgctgatga attgcagatg ctggtacaac aatatgccga tgacaaagag aaagtggcgc
     2401 tgttaaaagc actttggcag tacgcggaag agatcgtcta atttgaagcc gccgccgctg
     2461 gtacggcgga ggacaatgtt gagtcaggct ttttgaacgg tgatgctcca cgctgcatcg
     2521 ccaatctgct gataatcggt gatggcatgt ccttcttctg ccgcccactg tgggattgct
     2581 tcagttgcct gggtgcaatc aaactcaatt accaactcat cgccgctggc catttctgcc
     2641 agtgctgctt ttgcttctat gagtggaaac ggacagacct gcgtcaccac atccagcttt
     2701 ttaatcgcca tctttacacc ttacttaatt tgccgcagca gttgccagtc gcgccttacg
     2761 ctgcggtcga acatacacaa gccaggacgc agtccagact ccgagaatca taaatacaag
     2821 gccaatccag ccctgccagg tcatcattgc agtcataacc agtccattac cgatagagca
     2881 accacctgca atactggcac cgaagcccat cagtacacca cctaacccgc tacgtaatgt
     2941 tgtttgtgca tcagctgcgc gaacgcggaa ctcacggctc gctttggctg caataaatgc
     3001 ctccacgaag atccctaaca ccaggaaaac gccccagtta atgtatttca cgtcacccgc
     3061 gaccagaaat tgaagaatat tggccgttgg ggaagtgact cctaacccaa acatgcgtcc
     3121 ggttgcttca ctcaggggcc aggctaaaag cgcaatcaaa ccgatgagta cagcagtgac
     3181 aaagggatgc cagcgttttt caaacagaat atgagcgatc ccggttcggc gcggcggtaa
     3241 ggtcgcgact ttgagttttg gcttcttcaa ctctttcatc accacccaga gcgttatcac
     3301 cagcaaaacg gtaaccaacg gccacacaga caaattaaaa gtctcagcaa tagagttatg
     3361 ttcagtagtg tagtgctgca aggtttgatt taaaccactg gcgtgtggag aacgcatcac
     3421 cgcactcatc accatataag tgaaaagcgc gatccaactg ccgatcaatc cctcacccgc
     3481 gcgataccag gtcccggtgg cacatccacc cgccagaaca attcccagcc cgaaaagata
     3541 cccacctata acagtaccaa gccacgggaa cgccccggct tcgtaagtca gtaggcccgc
     3601 ctgaattaac gcaaaaaccc ccacgctttg tgccgaaatt gcaatcagca aggcgtaaaa
     3661 catgcgatta tttttcgcga tatacatatc gcgaaaacca ccagtcaggc aaaaacgccc
     3721 acgctgcatg acaaatccca gcagagcacc acaaattagc ccgcttaata tcattgaaaa
     3781 cataaattaa ttaaccagat gaatgttaat ggggaaattt ttcatgactg gtggaatgca
     3841 gaccaataac caaattctct aaattagaac aaatggttat taatgaggat ttcgataaaa
     3901 aagaataaaa aaaccggagc caaatgttca ttcgactccg gtttattatt agaaaagatg
     3961 attatgctac gtcttcgtac tgcggaaccg gattacggaa gctcttggtg acgcaagcca
     4021 ggtaaatcag accgatagca gcccagatca gacccagaac cattgagctt tcttccaggt
     4081 taacccacag cgcaccaacg gtcagcgcac cacacatcgg caggaacaga tactggaagt
     4141 gatctttcag cgtcttgtta cgcttctcac ggatccagaa ctgcgagatg accgacaggt
     4201 taacaaaggt gaacgccacc agcgcaccaa agttaatcag cgccgttgcc attaccaggt
     4261 cgaagttgat tgccagcagg gcaatcgcgc caaccaggat gatgttcatc gccggagtac
     4321 gccatttcgg gtgtacataa ccgaagaagc ttttcgggaa cacgccgtca cgacccatta
     4381 cgtacatcag acgcgctacg cctgcgtgcg ccgccatacc ggacgccagt acggtaatgg
     4441 tggagaagat cagcgcgcca acctggaacg ctttacccgc aacgtacagc atgatttcag
     4501 gctgtgacgc atccggatct ttaaagcgag agatatccgg gaagtacagc tgcaggaagt
     4561 aagttgcaaa gatgaagatc atgccgccaa tcagcgcggt caggaaaatc gcacgcggga
     4621 tcacgcgctc tgcatctttg gtt
//
LOCUS       DAJTSI010000224         4630 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000224 DAJTSI010000000
VERSION     DAJTSI010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.226"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            119..4237
                     /gene="pic"
                     /locus_tag="OO327_003862"
     CDS             119..4237
                     /gene="pic"
                     /locus_tag="OO327_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HCR6020217.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDKQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLGPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIILKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGIVVLNQ
                     QADTAGNIQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSQLLLDLKAQDTNVSEPTIGNISPFGGTGTPGNLYSMILNSQT
                     RFYILKSASYGNTLWGNSLNDPAQWEFVGMNKNKAVQTVKDRILAGRAKQPVIFHGQL
                     TGNMDVAIPQVPGGRKVIFDGSVNLPEGTLSQDSGTLIFQGHPVIHASISGSAPVSLN
                     QKDWENRQFTMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRAFVDKNDGTGNY
                     VIPEEGTSVPDTVNDRSQYEGNITLNHNSALDIGSRFTGGIDAYDSAVSITSPDVLLT
                     APGAFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNGKITLSGTPVKDTANQYAPAVYL
                     TDGYDLTGDNAALEITRGAHASGDIHASAASTVTIGSDTPAELASAETAASAFAGSLL
                     EGYNAAFNGAITGGRADVSMHNALWTLGGDSAIHSLTVRNSRISSEGDRTFRTLTVNK
                     LDATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMNNPAQGQALNIPLVTAPAGT
                     SAEMFKAGTRVTGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYK
                     NFMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELD
                     GVDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDY
                     TGNFASLGTKHYNTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGDM
                     DLSMKNRDFSPLVGRTGVELGKTFSGKDWSVTARAGTSWQFDLLNNGETVLRDASGEK
                     RIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
     gene            complement(4388..>4630)
                     /locus_tag="OO327_003863"
     CDS             complement(4388..>4630)
                     /locus_tag="OO327_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR6020218.1"
                     /translation="AYPTDIRKVIYTTNAIESLNSVIRHAIKKRKVFPTDDSVKKVVW
                     LAIQSASQKWTMPLKDWRMAMSRFIIEFGDRLDGHF"
BASE COUNT         1240 a         1050 c         1226 g         1114 t
ORIGIN      
        1 acaaaaacac attaatgcag taactatatt ttcctttctg gtgataacgt cgggttatca
       61 ttagcttctt cagctatttt acttttatat cccttgtaaa catcatggag aatccatagt
      121 gaataaagtt tattctctta aatattgccc cgtcaccggg gggcttattg ctgtctctga
      181 acttgcccgc agggtaataa aaaagacatg ccgaagatta acgcatattc ttctggctgg
      241 cattccagca atctgtctgt gttactctca gatatcccag gcgggtattg tccgttccga
      301 tattgcctat cagatttatc gtgatttcgc cgaaaacaaa gggctttttg tacctggtgc
      361 caatgatatt ccggtatatg ataaggacgg aaaacttgtg ggaagactgg gtaaagcccc
      421 aatggccgat ttcagcagtg tgagctcaaa tggcgttgct acgcttgtat cgcctcagta
      481 tatcgtcagc gtaaagcata acggaggata tcggagtgtg agctttggta atgggaaaaa
      541 tacatattcc cttgttgacc gtaataacca cccttctatt gacttccatg ctccacgtct
      601 gaataaactg gttacagaag ttattccctc agcggtaaca tcagaaggaa ccaaagccaa
      661 tgcttataaa tacactgaac gttacaccgc tttttatcgg gtgggtagtg gtacgcagta
      721 cactaaggac aaggacggaa atttagttaa ggttgccggt ggatatgctt ttaaaacagg
      781 aggaaccaca ggagttcctc tgatatctga tgcaacaata gtctctaatc ccgggcaaac
      841 ttataatcct gtaaacggcc ctttacctga ctatggagcc cctggggata gtggttctcc
      901 tttgtttgct tatgataaac aacaaaaaaa atgggttatt gttgctgtat taagagcata
      961 tgcaggtatt aatggtgcta cgaactggtg gaatgtcata ccaacagatt atctgaacca
     1021 ggttatgcag gacgatttcg atgcccccgt agactttgtt tccggactgg gccccctgaa
     1081 ctggacatac gacaaaacat caggcacagg taccctgagc cagggcagta aaaactggac
     1141 catgcacggg cagaaagaca atgacctcaa tgccggtaaa aatctggtat tcagcgggca
     1201 gaatggtgca attatcctga aagacagtgt gactcagggt gccggttatc tcgaatttaa
     1261 agacagttac accgtatctg ctgaatccgg aaaaacatgg acgggtgccg gcattattac
     1321 tgacaagggg acgaatgtaa cctggaaggt caacggcgtt gccggtgaca acttgcataa
     1381 gctgggggaa ggaaccctga ccataaacgg aacaggtgta aacccgggag gactgaaaac
     1441 gggagacggt atcgttgtac ttaaccagca ggcagacact gcaggtaata tccaggcctt
     1501 cagttcagtg aacctcgcca gcggacgtcc gaccgtggtg ctcggggatg cccgtcaggt
     1561 caatccggat aacatttcat ggggataccg gggaggtaag cttgacctta atggtaatgc
     1621 cgttaccttc acccgactgc aggctgctga ttacggggcg gtgattacaa ataatgcaca
     1681 gcaaaaatcc cagcttttac tggatcttaa ggctcaggat acaaatgtca gtgaaccgac
     1741 gattggaaat atatccccct ttggtggtac cggcactcca ggaaacctgt acagcatgat
     1801 actcaacagc cagacccgct tctatattct gaaatctgcc agctatggta acactctgtg
     1861 ggggaacagc ctgaatgatc cggctcagtg ggagtttgtt ggcatgaaca aaaacaaagc
     1921 agttcagaca gtaaaagata ggatcctggc cgggcgggca aaacaacccg ttatctttca
     1981 tggtcagctg accgggaata tggatgtcgc cattccacag gtgccggggg gaagaaaggt
     2041 aatctttgat ggtagcgtga acctgccgga aggtaccctg agtcaggaca gtggcaccct
     2101 gatattccag ggacatccgg ttatccatgc ctccatcagt ggcagtgcac cggtcagcct
     2161 gaaccagaaa gactgggaaa accgtcagtt tacaatgaaa acactgtcgc tgaaagacgc
     2221 tgacttccat ctttcacgta acgcctcgct gaacagtgac attaagtcgg ataacagcca
     2281 tatcacactg ggaagtgaca gggcatttgt ggataaaaat gacggaacag gaaattatgt
     2341 cattccggag gaaggtacct ctgtcccgga caccgtgaat gacaggagcc agtatgaagg
     2401 gaatattacg ctgaaccata actcagccct ggatatcggc agcaggttca ccggggggat
     2461 tgacgcttat gacagtgccg tcagcatcac ctctccggac gtcctgttga cagccccggg
     2521 tgcttttgcc ggcagttcac tgacagtgca tgatggcggt catcttacag cactgaacgg
     2581 tcttttcagc gacgggcata ttcaggccgg taagaacggc aaaatcaccc tgagcggtac
     2641 accggttaaa gatacggcta atcagtatgc ccctgctgta tatctgacgg acggatatga
     2701 cctgaccggc gataacgcag cactggaaat tacccgtgga gcacatgctt ccggtgatat
     2761 tcatgcctct gcggcatcaa cagttaccat cgggtctgac acgccggcag aactggcttc
     2821 tgcggaaacg gctgcatcgg cgtttgccgg cagtcttctt gagggctata acgcagcatt
     2881 caatggtgcc ataaccggtg gcagggctga tgtcagtatg cataatgcac tgtggactct
     2941 gggtggggac tctgccatcc acagtcttac cgtcagaaac agccgtatta gttctgaagg
     3001 agaccgtaca ttccgtaccc tgacggtgaa taaactggat gcaacaggca gtgattttgt
     3061 tttgcgtacg gacctgaaaa atgccgataa aattaatgtg actgaaaaag ccactggttc
     3121 agataacagc ctgaacgtca gctttatgaa taatcctgct cagggacagg ccctgaatat
     3181 tcctctggtc acggcaccgg cgggaacttc agcagagatg tttaaggccg gcacccgggt
     3241 gacaggtttc agtcgggtga ccccaaccct gcatgttgat accagtggtg gcaatacgaa
     3301 gtggatactg gatggtttta aagcggaggc tgataaagcc gctgccgcga aggctgacag
     3361 tttcatgaat gccgggtata aaaacttcat gacggaagtt aacaatctga acaaacgtat
     3421 gggtgacctg cgtgacacaa acggtgatgc cggtgcctgg gcgcgcatca tgagtggtgc
     3481 cggttctgca gacggtggtt acagtgataa ttacacccat gttcaggtcg gctttgacaa
     3541 aaaacatgaa ctggacggtg tggacctgtt taccggtgtc acgatgacct ataccgacag
     3601 cagtgcagac agccatgcat tcagcggaaa gacgaaatcg gtggggggcg gtctgtatgc
     3661 ttcagcattg tttgagtccg gtgcctatat cgatttgatt ggtaaatata ttcaccatga
     3721 caatgattac acaggtaact ttgctagcct gggaacgaaa cactacaaca cccattcctg
     3781 gtatgccggt gctgaaacgg gttaccgcta tcacctgaca gaggacacgt tcattgagcc
     3841 gcaggctgaa ctggtttacg gcgccgtgtc cgggaaaaca ttccgctgga aagacggtga
     3901 tatggacctg agcatgaaga acagggactt cagtccgctg gttggaagaa caggggttga
     3961 actgggcaag accttcagtg gtaaggactg gagtgtgacg gcccgtgccg gaaccagctg
     4021 gcagtttgac ctgctgaata atggagagac cgtactgcgt gatgcgtccg gggagaaacg
     4081 gataaaagga gagaaggaca gccggatgct gtttaatgtt ggtatgaatg cgcagataaa
     4141 ggacaatatg cgctttggtc tggagtttga gaagtcagcc tttggtaaat ataacgtgga
     4201 taatgcggta aacgcgaatt tccggtatat gttctgatgt gggggtaaag ggctgccgga
     4261 aggcagcctt tctcagagac tcttaaaata taaaactgaa ggtcttcggt aagggggagt
     4321 gtattagacg tcaactagtt ttgccgactg gcgcgagcct gtttaagatt ctgtgtaaat
     4381 gcctttctca gaagtgaccg tccaggcggt caccgaactc gataataaag cggctcattg
     4441 ccattcgcca gtccttcaac ggcatcgtcc atttctggga cgcagactgg attgccagcc
     4501 acaccacttt tttcaccgag tcgtctgtcg ggaacacttt acgctttttg atcgcatggc
     4561 ggatcacgct gtttagcgac tcgatggcat tcgtcgtata gatcactttg cggatgtccg
     4621 ttggataagc
//
LOCUS       DAJTSI010000225         4602 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000225 DAJTSI010000000
VERSION     DAJTSI010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.227"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(78..866)
                     /gene="phoH"
                     /locus_tag="OO327_003864"
     CDS             complement(78..866)
                     /gene="phoH"
                     /locus_tag="OO327_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCR6020219.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1473..2738)
                     /gene="efeB"
                     /locus_tag="OO327_003865"
     CDS             complement(1473..2738)
                     /gene="efeB"
                     /locus_tag="OO327_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCR6020220.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(2744..3871)
                     /gene="efeO"
                     /locus_tag="OO327_003866"
     CDS             complement(2744..3871)
                     /gene="efeO"
                     /locus_tag="OO327_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCR6020221.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(3929..>4602)
                     /locus_tag="OO327_003867"
     CDS             complement(3929..>4602)
                     /locus_tag="OO327_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCR6020222.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1081 a         1296 c         1109 g         1116 t
ORIGIN      
        1 ttcagaaaaa gcgctagtcc gggcataaac acccaaaaca aagcccggtt cgcccgggct
       61 ctgcaccgat aatacactta actgtatgca tgcagcgtac gttggcaaag tgccgaacgt
      121 acgcaatcct cttttccgaa ccggacgatc ccaaccattt catcttcttc gaaacgttcc
      181 agcgcgtcac ttaatccgga gcacacgccg cgaggcaaat cgcattgcgt gatatcaccg
      241 ttgacgataa ccgtcacgtt ctccccgagg cgggttaaaa acattttcat ttgcgcggca
      301 gtcacattct gcgcctcgtc aagaatgaca actgcatttt caaaggtacg tccacgcata
      361 taggcgaacg gcgcggtttc caccttccct atttccggtc gcaggcagta ctgcataaag
      421 gaagccccta agcgccggac cagcaagtcg tagaccgggc gaaaataggg agcaaacttt
      481 tctgcgatat caccaggtaa gaagccaaga tcttcatcgg cttgcagaac tggacgggtg
      541 acgataatcc tgtcgacatc cttatgtatc agggcctctg ccgcttttgc tgcgctgatc
      601 caggtttttc cgcacccggc ttcgcccgtg gcgaatatca gctgcttact ctcaatagcc
      661 tgcagatagt gcaattgcgc ttcatttcgc gcaaggatgg gcgaggtatc gcgactgtcg
      721 cgggccatac caatggcttc tacgccgctc atctgcacaa gcgaggtgac cgattcttct
      781 tcacgctgct tatggctgcg cgaatcccgt ctcagcacac gttttgcctc gcgacgagct
      841 ttgatcactg ctttttgtct tcccatggag agcaccttga gttgtttgta ttcatcacac
      901 gcgccgttgg cagcgcgatt atgcgcacga acatcagagg gttggcttcc ttgtaagcca
      961 tagtttgctt ttggataaaa tgccgaaaaa cggctacgca caccgtttgc ggcgtcggta
     1021 acacgagtaa agaaaggaag tgagaaaaat acagagtgac gaagttgcta ccggaggaga
     1081 aatctccgcg agtggtggag cgttgcttaa caaaacattg tccagaacag gacgttacca
     1141 tccgcgatct ccatagtgac tgactatcac tgccgggaac ttccgctgct acttaataag
     1201 tacaacagaa ctcgcattca ttgcaacaat atattcactt atatttaacg ataacaccca
     1261 tttcggtgac attaatttct actggaaaga tgacagagtg atgacagtga tggaaaaagc
     1321 tgtgtgcttt ccgcaggatt cgcagggctg gtaaaatgtg cctcgtgttt gccggattcg
     1381 gcgcgaacgc cttacccgac ctacaagtga ttaataattt gtgcacaaaa ctcatgcccg
     1441 ataaacctgc gcatcgggca tatttgcgtc gttcaaacct gcaatagcgc ccttcccaga
     1501 taatcgttcg cgtccttcac tcccggcagc gaaaaaaaat aaccgccgcc gataggttta
     1561 acgtattcct ccagcgcttc gccattgagc cttttttgta ctgtcaggaa gcctttttcc
     1621 agatcgtgtt ggtagcagac aaacagcaac cccatatcca gttgtccgga gttggtgacc
     1681 cccaacgaat aactgtagcc gcgacgcagc atcaggctgg actcactctc cgccgtgcgg
     1741 ggattcgcca gccggatatg gctgtccagc gcgatcacct tcccttccgg gtcgctggcg
     1801 taatcaggca catcatgctc atgctgcatt cccagcggcg caccggtttg cttatcacgg
     1861 ccaaaaatcg tctgctgttc tttcagcggc gttctgtccc aaaattccac tcgaaactga
     1921 atcaagcgta ctgcctgata gctgccaccg attgtccacg caggctcctg ctgatctgcc
     1981 gttacccaca ccactttttg catcaactta tcattctggc tatcgggatt ggcagtgccg
     2041 tctttgaaac ccagcaaatt aatcggcgtc tctttgcctt tactacgcgc cgcgtgatcg
     2101 gaaataaacc cttcccgctt ccagcgcaca ctgagcaaat ccggcgtgtg tttgatgata
     2161 tcgcgcagcg catggttcac cgtgtcctgg gtgttggcgc aaatctgtag caacacatca
     2221 ccatgacata acgccgcatc cagcgagtcg ttggggaaac gcgtcatctt ctgcagcttt
     2281 tttggcatct gtggcgcaag gccaaagcgc tcatcaaaca atgagtgacc caccgataac
     2341 gtgatggtga gattatcggg cgcaatgtag ccgccaagaa tgccggaatc gagtggtggc
     2401 aggcgcggat ttggcgtttc tggtgctgct ccgccctgac tcagaaaagc aaaacgctga
     2461 gtcaacaagc gaaacaaccg ctcaagatcg gctttatcgc tggcaagcac atcaaacgcc
     2521 accagcatca ttgcggcctg ttgtggcgtc aggatccctg cctgatgctc accataaaac
     2581 ggctgtttct cattgcgtgc atccggtgaa agcgtgcccg gtgcactttg cgttttttgt
     2641 gcatgagcga ccggacaact tcccgccagc gcaccaatcc ctttcagtaa acgtcggcgt
     2701 gacggttcat tcacgccgtt ttcatcttta tactgcataa cgcttaatcc agtcccagca
     2761 caccgcgaag ttgcgccaga tcttccgcca gcgcagtaat cggccctttc agcgcattcc
     2821 ggtcggcatc ggtcaatttg tcgtaggttt caaaaccgtc tttagtacgg tatttcgcca
     2881 gaatggtatc gaccttttta aagttggcat cgacttttgc caacagttcc gggttggctt
     2941 tttgcagttg tggacgcagc agatcgacaa ttttctgcga gccttcaacg ttagcctgga
     3001 aatcccacag atcggtgtgg ctgtagcgat cttcttcacc gctgattttg ctggctgcca
     3061 cttcctcaat cagtccggct gcgccgccga ccacttttga aggtgggaaa gccagttcac
     3121 tgatgcgttt ttgcaaatcg accacatcgg tataaagctg ctcagcgtac tgatccatcc
     3181 ctttggtggt gttgtcgcca aacaatgctt tttccagacg gtggaaacca gtgaattttg
     3241 ggtcggcggc tttttgctcg taatcatctt cacgggcgtc aatgctgcca tccagatcgg
     3301 agaacagttc agcaatcggt tcaatacgct catagtgctg gcgcgtcggc gcatacagtg
     3361 ctttcgcttt ttcgatatcg cctgctttaa tcgcgtcggt aaaggctttg gtgtcggtca
     3421 ccagctgcgt ggtttccgcc atgacatacg ctttatatgc agtaattgca ccaccaagac
     3481 ttaacagcgc atcactttgc gccgcatccg ccgttgcctc acctttgacg atcaacttcc
     3541 ctttcgggtt agtcagcaga ccgcaggtca tatcgtattc gccaggctgt aaattcgccg
     3601 tcattttctg gctaaaacca ggggcgatat tttcccgctc ttccaccacc atcacgcctt
     3661 tgaggatctc ccactccagc gccttctggc tatggttctg aataatgaac tgtgttttcc
     3721 cggcgttaac cgtaatggtc atcggttcgc actgcttatc cgtcacggtc actttgacct
     3781 gcggcacatc agcggcgtta gccataaaag cagaagaaaa cagcgcagcc acgctcaact
     3841 gcaatgcgtt acggcggaag ttaatggtca tgacgagact atccctttaa agtatgttgt
     3901 aactaagcaa gagtttgcgt cgtatttgtt acgcggagcg agacgctgtc gcccctgcgc
     3961 gtggtggcag agcaaatgcc accagcgccg ggatgagata aataaaccag acggcgactt
     4021 cgctgacgct cggtgcttcc tgatagccaa aaatcccttc catcagcgtg ccaaacagcg
     4081 agtgagttga gaggaccgcg ctcatatcga aggcgatttc ctgaaagtgg ttccacaatc
     4141 cggcttcatg aaatgcgcga atggcaccag ccgccagccc tgcggcgacg aagagaataa
     4201 acaggctggt ccatttaaaa aatgcaccaa gattgaggcg aataccgccc cagtagagca
     4261 ggaagccgag caccacggca gtagcaagac cgagcattgc acccagcggc ggccagatcc
     4321 cgacatcttg ttgaaatgcc gccagcagga aaaagaccga ctccagccct tcccttgcaa
     4381 cggcaaaaaa gaccatcatc accagcgccc agccatgatg atttccacgc tgcaatgcgc
     4441 tatcgactgc ctgttccagt tgcactttga cgttgcgcga cactttgcgc atccagaaaa
     4501 ccatccaggt aaggatcacc acggcgatca ccgccacgat accttcaaac agttcctgct
     4561 ctttttgcgg aaattcgccg gtggtttcgt taatgaagat cc
//
LOCUS       DAJTSI010000226         4530 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000226 DAJTSI010000000
VERSION     DAJTSI010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.228"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1222
                     /locus_tag="OO327_003868"
     CDS             <1..1222
                     /locus_tag="OO327_003868"
                     /inference="COORDINATES: protein motif:HMM:NF027197.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR6020223.1"
                     /translation="PYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLYP
                     LSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGISN
                     YAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGVT
                     NIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEKW
                     FIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILPV
                     IVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHLL
                     IANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRAA
                     RVFVNDERFTRGGN"
     gene            1222..1956
                     /locus_tag="OO327_003869"
     CDS             1222..1956
                     /locus_tag="OO327_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6020224.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
     gene            2423..3184
                     /locus_tag="OO327_003870"
     CDS             2423..3184
                     /locus_tag="OO327_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6020225.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3228..4307
                     /locus_tag="OO327_003871"
     CDS             3228..4307
                     /locus_tag="OO327_003871"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6020226.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
     gene            4304..>4530
                     /locus_tag="OO327_003872"
     CDS             4304..>4530
                     /locus_tag="OO327_003872"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6020227.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQNATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSI"
BASE COUNT         1243 a          862 c         1117 g         1308 t
ORIGIN      
        1 tccgtatggc attttccagc agcgaaatgt tcttgcgagc tggctggcaa caggttgcgg
       61 cgtagcgttg tatctggctt taacggcgcg tactcgttct ctgacgttga tatgtttgtt
      121 gtcgttatac cctctgtcta cagctttagt ccttacacaa agccgtgtcg gccttcttgg
      181 tttattttgc gtcatcgtgc tggccgctct cgcggacaga ccccgctggc gcggacgtcc
      241 ccttagcggt ctgttgcgct tgatcttcgt cgcctccttg ctgtgctggt gtgtcggtat
      301 ctcaaactat gccatgccag gggagacaac ggctgacttt acgcattcat catcgaatga
      361 ccagcgtata cgtattctga aaggtgccat aagtcttatt caacagcgcc ccttaaccgg
      421 ttgggggtta ggcagctttg aagcgacctt cccgcaagcc ttagaggatg tgggcgtgac
      481 aaacatagag agtgatacgg ctacgcatcc ccataacgaa ctgctgttcg taacggctga
      541 aggtggcatc gtcgccttgt ttggtttttt gctgctatca ggcgtttggc tgtggccgtg
      601 tatatgccgc ctgaaaatga caaacaataa cgcgcaccat caatgtgaaa agtggtttat
      661 tgccttaatg gggttgcctt tagtcattca tatgatgacc gaatatcctc tctatacgtc
      721 tgttccccat ttgatgctgt tgctgctcct tttccgcatc ggaatgccgg aaaacgtgat
      781 gaatcaggta cgtataccta aaacagtccg gttgggtatt ctgcctgtca ttgtgctcat
      841 tatgaccgcc gcgctggtca tcctggtagc gggatttgat gcacagcggg aactgaccag
      901 agctgaagct gatatgaacg aaggatttat ccctcgcctt ccggaacata gctggaaata
      961 cctgacgcag gcagaacgtc ttgagcgcga tcagcatctg cttattgcga ataaaccggg
     1021 gttcgttcgc gatacggaaa gcatgtctct tttcactgtg tggggaaata aatggttggc
     1081 aatccacaat gatgcagacg taagcgctgc aatgatattc attgcgcaca ggcgcggtga
     1141 ttatctgaca gctgaaaaaa taagaaaacg cgcagctcgg gtatttgtaa atgacgaacg
     1201 ttttactcgc gggggaaact aatggttaac gatctgacgt ataagaaatt agaaagcaca
     1261 aacatcaact gtaatattct ctcgccagaa aataccacat ggacaaatgt cacggtgatt
     1321 ggcctggatc tttttgccat ttgcggcgtg tgcagtttgc tcaagcgtgt tgggggagtg
     1381 aaacttcagg ttttcacttc cttacccact gatatgaatg ttctgactga cagtagcatc
     1441 gtgatatggg taaaaatgcg tcacgatggt ttgcctgaat tagccggaca tattgtgaag
     1501 gtttgtcgtc gataccgtct gatgcggcaa ctcgttatta gcgatgctat tcctgaagga
     1561 atgacgaatg atatcgggcc tctttcccga gtatggctta ctcatgggag tgcaaaagtg
     1621 gatattttat tatcttcatt acggaaggtg ttaaatgctc attgtttgcc tggtcctttg
     1681 tttataaaac gcctgggaag agtgcaatgg agggttcttt tactccgcgc gaaggggatt
     1741 agcacacgta aaattgcaga aatctgtggt attggcctca agaccgttag cgcccatgag
     1801 tccgcaattc gagaacgatt aggcaccagg aataaaagtg agtacgtctg gttagtacgt
     1861 aatgctcaac ttatacaaca ggcgataccc gcgttgggaa gagatgcgtc aaggtatgaa
     1921 agatgtaaca atatgcgaac aaagcaaaaa gtataattga tgcgtcatag ataactctct
     1981 ttttcgatga cgaaatcgct gagcatgcgt ggtgaggtta gtaatagatg ggtgctatgt
     2041 tttgccacgg gtttgtagct gtgaaacctg gaaaaaaata agaattaaaa atatttaaag
     2101 agagttaata aggttctggt tgtaattgtc ttggtcaaaa aaaagatatt gtcaatgttg
     2161 ttttttaaat tttcaggttt tggttaaata tttgcttgca aatataggtg attttttatt
     2221 gtaggctttt gaaggctatt ataactgtgc gctaaattaa tgcgttgaat aaaagaaata
     2281 acaaaattaa gaaaagccat atttacgata tgggcaacat aaatataatc taaaaaagag
     2341 taaggcgttg taggttggtt ccgcgttcat taccttgtta gtgaacgcgg atttatcagt
     2401 tgtcgaaatg gtttttataa taatgaaaaa ttttaatgta ctaaccaaag tcgtattttg
     2461 cttgtcgttt ttatcaagcg catttcccac ctatgctgcg gatgatgtca aagtgactca
     2521 aaacacttca gtttttaaac tgaagcttag tgcaacgcga ttaatctatg aacaagggtc
     2581 ggatggcgca gcgataactg tgacaaatcc gcaggattgg ccgatgcttg tgcagagtaa
     2641 agtctttgaa gaagataaag taactgcagc gccctttatt gtcactccgc cactgttcag
     2701 gcttaatcca ggccagcaaa ataaaatgtg ggttgtgcag actgcgccag ctttacaaga
     2761 cagggaaaaa ttacactggc tatgcgccac cggcattacg cctaaaaaga gtgatgtttg
     2821 ggccgagaaa gataaagaaa aaattgctga aaatgtgaac ctgaacatcc aggttaacgt
     2881 acatacctgc ataaaactta ttaccaggcc atctggtatt gaaggcaaga tggttaatgc
     2941 gagtgagaag ttatcgtgga aaaagcaggg tgataaaatt gtcgcggtca atcagtcgcc
     3001 gttctacatc aacctttctt ctttacaggt tggcggtagc aagattgaga aactagatta
     3061 tattgcgccg ttttcgcaac atgagtttgt tatgccaaaa actagcggcc aggatattaa
     3121 atggtctgtg attactgatg aaggtggtga gagtcgtttg tttatatctc atttgcaatc
     3181 ataatgtatt acatttttat tgaatatttt tatgccatgc atattccatg ttttatcgac
     3241 tgtcattaat tttgataatg gccttacttg cgggtcagtt aagtgcccag gagtggtcat
     3301 ttgattcttc acagttggaa ggaaacgtta gtgcggacac tgtggcaatg tttaatcagg
     3361 gagagcaact ccccggaaat tatcgtgtgg aaatttatct taacggtgaa aaagttgatg
     3421 ttggggagtt tccctttcat cgtccggaat cccctgaaga aaaagagctt gttccatgtt
     3481 taaccgttga tgaccttatc cactatggca ttaaaattga taaaagcagc agtgatactg
     3541 acaataaaaa gaatcagtgt tttaaatgga actctattga aggtttgaaa gtaaattatg
     3601 attttgatag ccagcgcgta cagataaccg ttccacaact gtatctgcaa gataaaaaaa
     3661 gtagtttagc accagtgtcg ttatggaatg agggtgtagc tgcattccgc atggtgtatc
     3721 agacaaatat tgatatttcc aaacaaaatg ataatcaatc taccacgcgt aattctcgtt
     3781 atggcaggtt cactccaggg tttaatcttg gtgcctggag gtttcgtagt tcagtgacat
     3841 ggagcaagga acttggccag tcagaaagat ggcaaagggg ttacatgtgg tttgagcgag
     3901 gcataaatgc gattaaaagc cgattaacgc ttggagaatc ttatacgtcg tctgaggtgt
     3961 ttgacagcat accttttcgc ggcgggatgt tagcgaccga cgatgcgatg actccgccgg
     4021 aggacagcta ctacacacct gtggtgcatg gaatagcaca aagtgaggcg caggttatta
     4081 ttaaacaaaa tgggcaaatt atttttaccc gttctgtgcc gccagggcct tttgcgctgg
     4141 ataatttacc aacgctggcc gttggcgggg agcttgatgt tacagtgaga gagagtaatg
     4201 gcgaggagca gtatttttcc gtgccatttc aaacgccggc catcgctctg catgaaggat
     4261 atttcaaata tagtgtgatg ggggggaata taaagaagaa ggtataaaaa gctcccctgt
     4321 ctccgtgggc gaaatcacgg cgatgtacgg gcttccttat ggcgtcacgg tgtatggagg
     4381 gattcaaaac gccactcatt ttaatgcaat atctacgggt attggcattt ctctggggct
     4441 gttagggagt ttatcgacag atattacgcg ttcgattgcg aatttatatt atgggaataa
     4501 atatcgtatt cgttatagta aatccattag
//
LOCUS       DAJTSI010000227         4434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000227 DAJTSI010000000
VERSION     DAJTSI010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.229"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..221
                     /locus_tag="OO327_003873"
     CDS             <1..221
                     /locus_tag="OO327_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR6020228.1"
                     /translation="QRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLPV
                     LQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            267..476
                     /gene="hha"
                     /locus_tag="OO327_003874"
     CDS             267..476
                     /gene="hha"
                     /locus_tag="OO327_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR6020229.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            515..1105
                     /locus_tag="OO327_003875"
     CDS             515..1105
                     /locus_tag="OO327_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR6020230.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1345..1605
                     /gene="repA"
                     /locus_tag="OO327_003876"
     CDS             1345..1605
                     /gene="repA"
                     /locus_tag="OO327_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCR6020231.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1829..1903
                     /gene="tap"
                     /locus_tag="OO327_003877"
     CDS             1829..1903
                     /gene="tap"
                     /locus_tag="OO327_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCR6020232.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1896..2753
                     /gene="repA"
                     /locus_tag="OO327_003878"
     CDS             1896..2753
                     /gene="repA"
                     /locus_tag="OO327_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCR6020233.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3454..3645
                     /locus_tag="OO327_003879"
     CDS             3454..3645
                     /locus_tag="OO327_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020234.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
     gene            complement(3875..>4434)
                     /locus_tag="OO327_003880"
     CDS             complement(3875..>4434)
                     /locus_tag="OO327_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS91-like element ISEc37 family transposase"
                     /protein_id="HCR6020235.1"
                     /translation="AAEGSSKREWNRFLDTHYRRGWNVNVSRVMDNATHVAVYFGSYL
                     KKPPVPMSRLEHYAGQDEIGLRYNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVR
                     YYGFLSPAKRRLLEEVVYIITETVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFT
                     GLKRGYRLAEQVLMHEPLARMRWCG"
BASE COUNT         1175 a         1137 c         1078 g         1044 t
ORIGIN      
        1 ttcagcgaga tcgttatggt cgtattctcg ggcaggttta tgcaccagac ggaatgaata
       61 ttaatcagtt tatggtccgg gccggggctg cctgggtata tgagcagtac aacacagatc
      121 ccgtgctacc tgtccttcag aacgaagcca gacaacaaaa acgtgggctc tggtcagatg
      181 ctgatccggt tcccccctgg atatggaggc accgtaaata aagcgatggc aatcctatca
      241 ttcatctgtt ctcagcggga ataaaaatgg caaaaacaaa acaggaatgg ctgtatcagt
      301 tacggcgatg ttcatcagtc aatacgctgg aaaaaattat tcataaaaac agagacagtt
      361 tatcaaacag tgaacgtgaa tcatttaatt cagcagcaga ccaccggcta gcggaattaa
      421 tcaccggaaa gttatatgac aggataccaa aagagatatg gaaatatgta aggtaataat
      481 tcagcaataa ttaattgcca ggagcagata aataatgaag ttgattattt ttattctcat
      541 tgttctgatt attgcagcgc tacttatccg gattatcctc agaagtgtaa atcagcattc
      601 tcctttgctg atgcaattgc atgcggcagg tatacggact ggtgatgcag agcgaatact
      661 gtccagcggt gaatactggc aacgtcagaa gacgctgctg acagaaaggg aagtcagttt
      721 tatgaaagga ctgttcagaa ttgtggatat gaagcggtgg tatctgtgtc cgcaggtacg
      781 ggtagcggat atcgtccagc tgaacgggaa tatccggcca cgatcacgcc agtggtggca
      841 gttattcagg atggtgtctc agtggcatgt tgatgtggtt atcgttgagc tgcgttcgtt
      901 cagtattgtt gcagcagtag agctggatga tgccagccat ttacgaccgg aacgcagacg
      961 ccgggatatt cttctggaag aggttctgag gcaggctggt attccgttgc tcagaagcca
     1021 cgatgccaga aaactgctgc agatgaccgg agaatggctg aatacaacag gggctgatca
     1081 gcagtcaccg gaacatcgta gctgacgcct tcgcgttgct cagttgtcca accccggaaa
     1141 cgggaaaaag caagttttcc tcgctcccgg cgtttccata actgaaaacc atactatttc
     1201 acagtttaaa tcacattaaa cgacagtaat ccccgttgat ttgtgcgcca acacagatct
     1261 tcgccacaat tctcaagtcg ctgatttcaa aaaactgtag tatcctctgc gaaacgatcc
     1321 ctgtttgagt attgaggagg cgagatgtcg cagacagaaa atgcagtgac ttcctcattg
     1381 agtcaaaagc ggtttgtgcg cagaggtaag cctatgactg actctgagaa acaaatggcc
     1441 gctgttgcaa gaaaacgtct tacacacaaa gagataaaag tttttgtcaa aaatcctctg
     1501 aaagatctca tggttgagta ctgcgagaga gaggggataa cacaggctca gttcgttgag
     1561 aaaatcatca aagatgaact gcagagactg gatatactaa agtaaagact ttactttgtg
     1621 gcgtagcatg ctagattact gatcgtttaa ggaattttat ggctggccac gccataaggt
     1681 ggcagggaac tggttctgat gtggatgtac aggagccaga aaagcaaaaa ccccgataat
     1741 cttctttaac tttggcgagt cagaaagatt accggggccc acttaaaccg tatagccaac
     1801 aatcaagcta tgcggggagt atagttatat gcccggaaaa gttcaagact tctttctgtg
     1861 ctcgctcctt ctgcgcattg taagtgcagg atggtgtgac tgatcttcaa caaacttatt
     1921 accgccaggt aaagaacccg aatccggtgt tcacaccccg tgaaggtgcc cggacgcttc
     1981 cgttctgcgg gaaactgatg gaaaaggcgg tgggcttcac ctcccgtttt gatttcgcca
     2041 ttcatgtggc acatgcccgt tcgcttggtc tgcgtcggcg catgccaccg gtactacgtc
     2101 gccgggccat tgatgcgctg ctgcaggggc tgtgttttca ctatgaccca ctggccaacc
     2161 gcgtccagtg ttccatcacc acgctggcca ttgagtgcgg actggcgacg gagtctgctg
     2221 ccggaaaact ctccatcacc cgggccaccc gggccctgac gttccttgca gagctgggac
     2281 tgattaccta ccagacggaa tatgacccgc ttatcgggtg ctacattccg accgatatca
     2341 cgttcacatc tgcactgttt gctgccctcg atgtatcaga ggaggcagtg gccgccgcgc
     2401 gccgcagtcg tgtggaatgg gaaaacagac agcgcaaaaa gcaggggctg gataccctgg
     2461 gtatggatga actgatggcg aaagcctggc gttttgttcg tgagcgtttc cgcagttacc
     2521 agacagagct taagtcccgt ggaatgaagc gtgcccgtgc gcgtcgtgat gcggacaggc
     2581 agcgtcagga tattgtcacc ctggtgaaac ggcaactgac gcgtgaaatc tcggaagggc
     2641 gcttcactgc cagtcgtgag gcggtaaaac gtgaagtgga gcgtcgtgtg aaggagcgca
     2701 tgattctgtc acgtaaccgt aattacagcc ggctggccac agcttccccc tgaaagtgac
     2761 ctcctcagaa taatccggca cgcgccggag gcattcgcac gcctgaagcc cgccagtgca
     2821 caaaaaatca gcgctacata caaaaaacaa cctcatcatc caccttcagg tgcatccggt
     2881 cccccctgtt tttgatacaa aacacgcctc acagacgggg aattttgctt atccacatta
     2941 aactgcaagg gacttcacca taaggttaca accgttcacg tcataaagcg ccagacgtca
     3001 accctacagg gtgcaatgta tcttttaaac acctgtttat atctccttta aactacttaa
     3061 ttacattcat ttaaaaagaa aacctgttca ctgcctgtcc tgtggacaga caaatatgca
     3121 cctcccaccg caagcggcgg gcccccaacc gaagccggtt tagttacaac atgcaaacat
     3181 aaacccctaa aaaacccaag tccaacgcaa aactgaaacc acaacgcccc cctcataact
     3241 gaaaagcgcc gccgcccccg cccaaagggc gggaacagag tcgcttttaa ttatgaatgt
     3301 tgtaactaca tcatcatcgc tgtcagtctt ctgcctggac gtatcgaata cacgctcgta
     3361 agcggccctc acggcccgct aacgcggaga tacgccccga cttcgggtaa aaccctcgtc
     3421 gggaccactc cgaccgcgca cagaagctct atcatggctg aaaacgggta tggcttggca
     3481 gggctgggga tgggtaaggt gaaatctgtc aatcagtacc ggctgacgcc gggcttcggc
     3541 ggttttactc cggtatcaca tgtaaccaca gcgtgccgcc ttccatgccg ctggcgcggc
     3601 atcagaataa tacaagcagc atttaatgca tttgcaaaag tgtgagtaat attaagtggt
     3661 cgtttcaggg atatcgttaa gacttgagcg ataggaatta aaaccccaaa aagattaaaa
     3721 aaacaccaca aaacggatgt ttcttcaaca ccacttttgc tccatatgaa cggaaccgac
     3781 gattaaactg gatggctctg attgattcag ggtatgaatg gcggtttttt gctccgtttc
     3841 cctcaaaatg gacgcaactt cccctctgcg gctctcagcc gcaccaccgc atccgggcca
     3901 gcggctcatg catcaggacc tgctctgcca gacggtagcc ccgcttcagc cccgtaaaac
     3961 gcatctgact cccgcacagc acgcacttca gcgggtcaac cttcagtaac ctctgataca
     4021 tccctctcca ggtgatttgc atcgccgttt ttctcactgt ctccgttatg atgtacacca
     4081 cttcttccag taaccgccgt ttcgccggac tcaaaaaacc gtagtacctc accatacgga
     4141 accccttatc cgccacatgc caggagaacc tttccatgaa ctcatctcca ctcatcaaca
     4201 ggtattcttc ccgttttgtt cggtgactgt tgtaacgcag accgatttca tcctgaccgg
     4261 cataatgctc cagacgactc atcggcaccg gtggcttttt caggtaagag ccaaagtaca
     4321 ccgccacatg ggtggcatta tccattaccc gggatacgtt gacattccag ccacggcggt
     4381 aatgcgtgtc caggaagcga ttccattccc gtttactgct tccttctgct gcca
//
LOCUS       DAJTSI010000228         4239 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000228 DAJTSI010000000
VERSION     DAJTSI010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.232"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..446
                     /locus_tag="OO327_003881"
     CDS             <1..446
                     /locus_tag="OO327_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR6020236.1"
                     /translation="SACAPETGLQQLVATPVPDEQRISFWPQHFGLIPQWVTLEPRVF
                     GWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGNRAEMSPEAAG
                     IAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            462..938
                     /locus_tag="OO327_003882"
     CDS             462..938
                     /locus_tag="OO327_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR6020237.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            1001..1222
                     /locus_tag="OO327_003883"
     CDS             1001..1222
                     /locus_tag="OO327_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR6020238.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            1385..1759
                     /locus_tag="OO327_003884"
     CDS             1385..1759
                     /locus_tag="OO327_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCR6020239.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            1806..2180
                     /locus_tag="OO327_003885"
     CDS             1806..2180
                     /locus_tag="OO327_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR6020240.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            2177..2668
                     /locus_tag="OO327_003886"
     CDS             2177..2668
                     /locus_tag="OO327_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020241.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            2680..2877
                     /locus_tag="OO327_003887"
     CDS             2680..2877
                     /locus_tag="OO327_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020242.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            2962..3522
                     /locus_tag="OO327_003888"
                     /pseudo
     CDS             2962..3522
                     /locus_tag="OO327_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            3594..>4239
                     /locus_tag="OO327_003889"
     CDS             3594..>4239
                     /locus_tag="OO327_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HCR6020243.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTI"
BASE COUNT         1032 a         1152 c         1059 g          996 t
ORIGIN      
        1 cttcggcctg tgcaccggag actggcctgc aacaactggt tgccactccc gtccctgatg
       61 aacagcgcat cagcttctgg ccgcagcatt ttggcctcat tccacagtgg gtcaccctgg
      121 aaccccgtgt cttcggctgg atggaccgtc tgtgtgaaga ctactgcggt ggtatctgga
      181 atctgtacac cctgaacaac ggcggggcat ttatggcacc cgaaccggat gacgatgatg
      241 acgaaacatg ggtactgttc aatgccatga acggtaaccg cgctgaaatg agcccggaag
      301 ccgccggcat tgctgcctgt ctgatgacgt acagtcatca tgcctgtcgt acggagtgtt
      361 atgccatgac ggtccattat taccggctgc gggattacgc cctgcagcat ccggaatgca
      421 gcgccattat gcgcatcatc gactgaatgg aggcagggac aatgcagcag ctttcctttc
      481 tgcccggaaa catgacgccc ggcgagcgca gtctcattct gcgggccctg caaaccctgg
      541 accgccatct tcatgaaccc ggcgtggcct tcacctccac ccgtgcggca cgggaatggc
      601 tgattctgaa catggcggga ctggagcggg aagagttccg ggtgctgtat ctgaacaacc
      661 agaatcagct gattgccggt gaaaccctct tcaccggcac catcaaccgt acggaagttc
      721 atccccggga agtgattaaa cgcgccctgt accacaatgc cgctgccgtg gtgctggcgc
      781 acaatcaccc gtccggtgaa gtcacaccca gtaaggcaga ccggcttatc accgaacgtc
      841 tggtacaggc actgggcctg gtggatatcc gggtgccgga ccatctgata gtcggtggca
      901 accaggtttt ctcctttgcc gaacatggtc tgctttaacc cgtcacaacc acatcacacc
      961 tgttttcact tttatcttct gtcttcagag gtatcacatc atgagaatca tcacccgtgg
     1021 tgaagccatg cgtattcacc aacagcatcc tgcatcccgt ctttttccgt tctgtaccgg
     1081 taagtatcgc tggcacggca gtgctgaagc gtatactggt cgtgaagtgc aggatattcc
     1141 cggcgttctt gccgtgtttg cagaacgccg taaggacagt tttggcccgt atgtccggct
     1201 gatgagtgtc accctgaact gatgttccgt cacccgggaa acgagtacga ccagatgacc
     1261 tcagacgccg ctgtggatga ggccattctc atcaatgcat acatcttcac cgaaaccggt
     1321 cagcgcgccg gctgacctgt catcgttaaa ccattcttaa ccacttttca gagaggattt
     1381 tatcgtgtca gacaaactct ccgggataac tcatcccgat gacaatcacg accgcccctg
     1441 gtgggggctt ccctgcacag tgaggccctg ttttggcgct cgtctggtgc aagagggtaa
     1501 ccgcctgtat taccttgccg accgcgccgg tatcagaggc cgcttcagcg acgcagatgc
     1561 ataccatctg gaccaggcct ttccgctgct gatgaaacaa ctggaactca tgctcaccag
     1621 cggtgaactg aatccccgat atcagcatac cgtcacgctg tatgcaaaag ggctgacctg
     1681 cgaagccgac accctcggct cctgtggtta cgtttatctg gctgtttatc cgacaccagc
     1741 acccgcaacc acctcataac cccgaagctc tcacctttag cactaccagt tacagagaac
     1801 ttaagatgaa aacattatcc gacacacatg tacgggaggt atctcgctgc ccgtctcccg
     1861 tcaccatctg gcagacactg ctcatccgac tgctggacca gcattatggc ctcacactga
     1921 atgacacacc gtttgtcgat gaacgtgtga ttgagcagca tattgaggca ggcatttcac
     1981 tgtgtgatgc agttaatttt ctcgtggaaa aatacgcgct ggtgcgtacc gaccagccgg
     2041 gattcagcac ctgtccccgc tctcagttaa taaacagtat tgatatcctc cgggctcgcc
     2101 gggcgaccgg cctgatgacc cgcgacaatt acagaacggt aaataacatt accctgggta
     2161 agtatccgga ggcgaaatga tgaaactggc cctgacactg gaagccgaca gcgttaacgt
     2221 acaggcactg aacatagggc gcattgtcgt tgacgtcgat ggtattgagc tcgctgaact
     2281 gattaacatg gtctgcgata acggctactc ccttcgtgtt gttgatgaat ctgaccagac
     2341 ctcagcagaa tgcacgccac catttgctac ccttaccggc atacgctgca gtaccgcaca
     2401 tatcacggaa acggacaacg cctggttgta ctcgctgtca caccagacca gtgacttcgg
     2461 tgaatcagaa tggattcatt ttacaggtag cggatatctg ttacgtaccg atgcgtggtc
     2521 atacccggtt ctgcggctta aacgcctggg gctgtcaaaa acgttccgtc gtctggtcgt
     2581 cacactcatc cggcgttatg gcgtcagtct cattcatctg gatgccagcg ctgaatgcct
     2641 gccgggttta cccactttcg actggtaacc aggaacaaca tgaaatcatt aaccacggaa
     2701 acagcgctgg atattctgat tgcgtggctg caggacaata tcgactgcgg atcgggaatt
     2761 atctttgaca acgatgagga taaaacggat tcagcagcac tgttgccctg tatcgaacag
     2821 gcccgggagg atgtccgtac cctgcgtcat ctgcagcttc tgcaccagaa ccggtgaagc
     2881 tcactcacca gacttcattc ctctgacgcc agcctgcaca gggctggcgt tttcatttat
     2941 ctgcaaaaag gaatatcgat tatgtcagat atcacaatct cccgtccgga agtggtcaac
     3001 gggcatacgg acgttatctg ctcaaccagc attcgccaca ttctggctgt acgaaagagt
     3061 acactgttgc aaatcgacac acttatccga caactggctg aaatctcggt gctgacggaa
     3121 agcatcggag gtaaaactgc cccggactgg gccatgaaac aggattttcg ctgcggctgc
     3181 tggctgatgg agaaacccga aacagcaatg aaagccatta cccggaatct tgatcgcgaa
     3241 atctggcgtg acctgatgca acgttccggg atgctgtcat taatggatac gcaggcccgt
     3301 gatacatggt accggtcact ggagtacgat aattttccgg aaatcagcga agcgaacatt
     3361 ctgagcacat ttgaacaact gcaccagaat aaggatgagg tgtttgagcg aggggtgatc
     3421 aacgtcttca gagggctgaa ctggaattac aaaaccaatt gcccctgtaa atttggcagt
     3481 aaaattatcg tcaacaatct ggtgagatgg gaccggtggg gataatgata tggtcacccc
     3541 caccttaaca gcagtacgct gttaaggtaa atccctttaa cggagagacc atcatgcaga
     3601 acatcacact tattggtatc gatctcggaa agcactcctt ccacattcat tgtcaagaca
     3661 agtctggtaa ggctcttctg cgtaaaaaat ttacccgtac taaactgatg cagtttctgg
     3721 cgacatgtcc gccctcagtt gttgtaatgg aagcctgtgc gggtgcacac tttatggctc
     3781 gctgtatctc tgattttggg catgaaacta agctgatttc tccacagttc gttcgcccgt
     3841 ttgtcaaaag taataagaat gattttgttg atgcagaagc catatgcgaa gcggcttcac
     3901 gaccatcaat gcgttttgtc cagccaagaa ctgagaccca gcaggccatg agggcgttac
     3961 accgggtcag agaatcgctc atcagagata aggtgaaaac aaccaaccag atccacggtt
     4021 tcttgctgga atttggaatc agcctgccga taggtgatgc tgtaataaaa cggttgtctc
     4081 tcgtgttggc tgaacacgaa atccccgaat atctgagccg ccttttggtg aggttgcata
     4141 cccactactt ttaccttgtt gagcaaatag cagagctgga atcagaatta agtcagtcaa
     4201 tcaacgctga tgataccgct cagcgcatta tgacaattc
//
LOCUS       DAJTSI010000229         4200 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000229 DAJTSI010000000
VERSION     DAJTSI010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.233"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            85..753
                     /locus_tag="OO327_003890"
     CDS             85..753
                     /locus_tag="OO327_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020244.1"
                     /translation="MSEQIKQDIDLIEILFHLKKKIRVILFIMAICMAMVLLFLYINK
                     DNTKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDIKNREIR
                     LEWSGDKRDLPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(996..1691)
                     /locus_tag="OO327_003891"
     CDS             complement(996..1691)
                     /locus_tag="OO327_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCR6020245.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1684..3111)
                     /locus_tag="OO327_003892"
     CDS             complement(1684..3111)
                     /locus_tag="OO327_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCR6020246.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3122..3841)
                     /locus_tag="OO327_003893"
     CDS             complement(3122..3841)
                     /locus_tag="OO327_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCR6020247.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1120 a         1085 c          869 g         1126 t
ORIGIN      
        1 tttgctgata tttaacccga acattcctga tacagactat taatacaaca ttatctgcga
       61 cagattttat tttctggata tattatgagt gaacaaatca aacaggatat cgatctgatt
      121 gagattttat ttcatctgaa gaaaaagatt cgtgttatcc tttttattat ggctatctgt
      181 atggcaatgg tgctgttgtt tctgtatatc aataaagaca atacaaaagt gatttacagc
      241 cttaaaataa accagacaac gccaggtata cttgttagct gtgatagcaa taataatttt
      301 gcctgtcaga ccacaatgac tgaagatgtt attcagcgaa ttactacatt ttttcagacc
      361 agcccagata tcaagaacag agaaataagg ctggaatggt caggagataa gagagattta
      421 ccaactgctg aagaggaaat atctcgcgtg caggcgtcta ttatcaaatg gtatgcgtca
      481 gaataccata atggcaggca agttctcgat gagatacaaa cgccttcagc aattaacagt
      541 gagctttata caaaaatgat atacctgacc aggaactggt cattgtatcc gaacggtgat
      601 ggctgtgtaa ctataagctc accagaaata aaaaataaat accctgctgc catttgcctg
      661 gctctgggat tttttctaag cattgtaatt tctgtaatgt tttgccttgt caaaaaaatg
      721 gtagatgaat accaacaaaa ctctgggcag taataaacag cattctgata gagagtgcct
      781 gtataaagct gaagtgacaa tgaccgggaa gggaacccgt tttcaggaat ggatgcctgt
      841 ctttttcact gtaaatatcc tggcattact aatgcctgga tatttacaga agagcagcat
      901 aaccatgacg gaacggggtt aagtcaggtt gtccttaacg ctattttcaa cctcgtatgc
      961 cttcttcagg tttatgttca gatctcatat ctctctcagc aatcctcaat aatcagatac
     1021 accgctttca ccggaccatg aaccccgacg actttgataa gctcaatatc cgccgttgag
     1081 ctgggaccac tgatgatgtt aatacatgac ggcattcgtt caccggcctg cgctttctga
     1141 tgcaattttt ctgcgagttg cgctacacgc ggcaggattg tgcttttacg caggataaaa
     1201 agagaagatt ccgggagcag gctcaatgaa cgcccgcgct cggcggcgga aaaaagaacc
     1261 acgcctcccg attcggttaa accatattca gcatacacga cacccacttt agcctgctct
     1321 gcctgcgaga tattctcggc acctttcgcc ggatcccaaa caacggcatt gcattcctgc
     1381 tgcaaacgtt cactaatccc caattcctcc agcctcgtgt caccgctaat cacgaccgac
     1441 tgatctccca gctctttaca cagacgtatt gcagcttctg ccgccttcgc ctcgctggtc
     1501 agctcacagc gcgtcaacat aacatcgctg gcaaactgaa taaacgcgtc acagcgctgc
     1561 tgttggttaa gttgggtaag ccgctcgtta gcatagttgt taagcggcgc atcttctgct
     1621 tgcggttcaa gtcgcagcgg gcgacccagt gcctgagcaa cgttattcaa aaattcgctc
     1681 cgattatcca ttctttttct cctgcgcctg atgtttctta aaccaactac ggaaactctc
     1741 tccgtcagct tcaggaagat cgcgtgcttc catccagtcg ctaatcgcgc caaatttgag
     1801 tggtgttttg ccgccattga taaaccagct tgccgcatga gcgccggcca tcatcccgac
     1861 tttccacaat cctggatgac tattggcata agcgaacatt tttatcgccc gttgctctgc
     1921 ttttgcggtg atcccttttt cagccatcac ccgacgatga cgcaaaatca gttttgacag
     1981 cggaatacgc accggacaca cgctgtcaca agcagtgcat aaagagcagg cgtagggtaa
     2041 atctttaaaa tctttatagc cgccaagtag cggagaaatc accgcaccaa ttggccctgg
     2101 ataaatagag ccatatccat gaccgccaat atggcgatat gccggacaag tattcataca
     2161 agccccgcag cgaatacagc gcagcacatc ccgaaattca gaggccagca cctcagaacg
     2221 cccgttatcg acaataacca gatgaaactc ttcaggacca tcaacgtgcc ccgcttcgcg
     2281 cggccctgtc agccaggtgt tgtatcccgt caaacgtgca ccaacggcac tgcgcgccag
     2341 catggtgatc aatacatcta cctcggcaaa cgtgggggca atacgctcca ttcccatcac
     2401 tgcaatatgc gttttaggca gcgtggtaca cattcgcgca ttaccttcat tggtcaccag
     2461 gcataccgaa ccggtctctg ccaccgcgaa attacagccg gtaataccta tttcagcact
     2521 gaggaaatct tcacggattt tttgccggat gaataaggtc atcgcttcag gcgtttccgg
     2581 cccctcatag cccagacgtt cgtgtagcac tcgacggatc tgatggcgat ctttatgaat
     2641 tgccgggacc acaacatgag atggcggatc ttgatccagc tggagaatat attcacccag
     2701 atcggtttca atcacctgaa tgccagcatc ctgcaacaca tgattgacac caatctcttc
     2761 ggtcaccatc gatttagatt tcaccacctt ccgggcattt ttgcgttggg caacctgtaa
     2821 aatgtagcgg gtagcgtctt ctttggtttt tgcaaaatag acgtgaccgc cgttttgcgt
     2881 cactttttct gagagctggt acagataagc gtcgagatta ctcagaacat gatcacgtat
     2941 ctgggcggcc cgatcgcgcc actcctccca gtgccccaat tcatcgacca ttttttgccg
     3001 atttgcccca atacgctgct gcgcgcttgc caccgctttg cgcatgatcg gatcttcaat
     3061 ttgctgacgg atgcgtgtct taaaatctgt attactggtt ttgatcgaca tctttatatc
     3121 ctcagcggct catcaacact tcagcaatat gcatcacttt gactttctgc ccttcccgtt
     3181 gtaatcgccc actgatgttc agcaggcaac tcacgtcagc accaattaaa tactcagggc
     3241 ggacttccat caggtgcaca accttttctt tcaccatctc gccggatatt tcggccattt
     3301 tgaccgagaa cgtgccgcca aatccgcagc aggtatcctg ttcagcaaag gtcaacagct
     3361 ccagtccacg cacatttttc agcagcgtaa gtggctcgtc cttcactccc agcttacggg
     3421 ccaggctaca agatgggtga tacacagctc tcccttgcaa actggcacct acatcgacta
     3481 cccctaattt attaacaata aaagaggtga gatcctgcat acgcgcggca accttttcgg
     3541 cacgtgatgc ccattcaggt tcatccgcca gatacgtcgg gtaacttttt atggcatagg
     3601 tgcaagagcc agccggtgaa ataatgggat cgtcgttatc ctccagtgcg gcgatcagat
     3661 ttttcatccc tggaatcgct tctttgatat aaccgctatt gatcgcaggc tgaccgcagc
     3721 atccctgttt ctccgggaaa tttacgcgac agccgagttt ttccagtagc agcacggagt
     3781 ctcgtgccat tcttgatttc agggcgtcac caatacaggt gacaaagaaa ttgacattca
     3841 caactaatac tccattactt catgcccatt tatgcccgat gtttcccccc caccggggga
     3901 tcaatcatca ataaatatgg cgttcttttt taattggcta gcaacatacc acccctcatt
     3961 tgtatgacaa atgataatct ttcatccaaa cttcaaatgc aataactaat tttatttctt
     4021 tcggcatcgc tcacatttta tggggaaatc gacgatcatt tttggcataa gaaatccatt
     4081 taaaaatggc tacatccctt atacctattc agagctactt attcaggggt tattgagata
     4141 tacactgatg gtgtacaaaa aaatcaccga caataatcac gtatcaagta atagtcggaa
//
LOCUS       DAJTSI010000230         4199 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000230 DAJTSI010000000
VERSION     DAJTSI010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.234"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..199)
                     /gene="tuf"
                     /locus_tag="OO327_003894"
     CDS             complement(<1..199)
                     /gene="tuf"
                     /locus_tag="OO327_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6020248.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTS"
     gene            complement(314..389)
                     /locus_tag="OO327_003895"
     tRNA            complement(314..389)
                     /locus_tag="OO327_003895"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(354..356),aa:Thr,seq:ggt)
     gene            complement(396..470)
                     /locus_tag="OO327_003896"
     tRNA            complement(396..470)
                     /locus_tag="OO327_003896"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(435..437),aa:Gly,seq:tcc)
     gene            complement(587..671)
                     /locus_tag="OO327_003897"
     tRNA            complement(587..671)
                     /locus_tag="OO327_003897"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(635..637),aa:Tyr,seq:gta)
     gene            complement(680..755)
                     /locus_tag="OO327_003898"
     tRNA            complement(680..755)
                     /locus_tag="OO327_003898"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(720..722),aa:Thr,seq:tgt)
     gene            1117..2067
                     /gene="coaA"
                     /locus_tag="OO327_003899"
                     /gene_synonym="panK"
     CDS             1117..2067
                     /gene="coaA"
                     /locus_tag="OO327_003899"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR6020249.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2096..3061)
                     /gene="birA"
                     /locus_tag="OO327_003900"
     CDS             complement(2096..3061)
                     /gene="birA"
                     /locus_tag="OO327_003900"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR6020250.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3058..4086)
                     /gene="murB"
                     /locus_tag="OO327_003901"
     CDS             complement(3058..4086)
                     /gene="murB"
                     /locus_tag="OO327_003901"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR6020251.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1086 a         1044 c          936 g         1133 t
ORIGIN      
        1 gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg atctggtcga
       61 atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca gcggtcagcg
      121 ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt tttgtacgtt
      181 caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg tgatatcacc
      241 acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga aaaaacaggg
      301 aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc cttaccaagg
      361 gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg gaatcgaacc
      421 cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc atcctagaac
      481 tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt attcgagccg
      541 gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt ggtgggggaa
      601 ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt tggccgctcg
      661 ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac ctactgatta
      721 caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca ttctatggag
      781 acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca cattttatgc
      841 tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca acatcattgt
      901 gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat atctgtcaca
      961 ttgcttttca acgatagctt cctggcagag attttttctt attattcctc cccatctggt
     1021 gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg caaatatcgc
     1081 tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga gcaaacgtta
     1141 atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga ttccgtacct
     1201 atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga tctctcgtta
     1261 gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta tataagctcg
     1321 aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca acgcattcct
     1381 tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc ccgtgtattg
     1441 caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac tacagatggc
     1501 ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa aggcttcccg
     1561 gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc cggcgtgcca
     1621 aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga tggagataaa
     1681 actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca gagcgggatg
     1741 gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt ttcgatatat
     1801 gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct gaaattccgc
     1861 gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt aactaaagaa
     1921 gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa cttaaagcaa
     1981 aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc taatcatgtg
     2041 gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc ctttcttatt
     2101 tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt ccatcctgct
     2161 caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt tctttatcac
     2221 caatgataag tttcactggg cgattaataa aattatccag cttttcccag cgcgacagat
     2281 aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca cgtattagca
     2341 tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg atccacccct
     2401 gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct ccaatgacta
     2461 tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc agcttgcgat
     2521 cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc agctttcgta
     2581 atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc gccgggcctt
     2641 gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa aaccatttcc
     2701 gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg cccgatttaa
     2761 gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc agcacggcta
     2821 cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg atgggctcag
     2881 gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca cgcagtgtct
     2941 gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac tgctcgccag
     3001 agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg ttatccttca
     3061 tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga cttcaggctc
     3121 aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca gttgcaccac
     3181 atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac ggtgcaccgc
     3241 agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac ctgctgccag
     3301 ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg ccagtaatgc
     3361 tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat tgccattcac
     3421 ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata cttgctgtgg
     3481 cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag gttgccactc
     3541 ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt catgtttaaa
     3601 aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt gcttgcctgt
     3661 cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc cataagcacc
     3721 aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat tttccagacc
     3781 aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc cggctcctac
     3841 atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc ggttgatgat
     3901 caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca gaataagaac
     3961 gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt cgtcttcggc
     4021 acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt ttaaggagtg
     4081 gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg tggggggatg
     4141 gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggcccg actgggctt
//
LOCUS       DAJTSI010000231         4183 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000231 DAJTSI010000000
VERSION     DAJTSI010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.235"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(348..995)
                     /gene="ychE"
                     /locus_tag="OO327_003902"
     CDS             complement(348..995)
                     /gene="ychE"
                     /locus_tag="OO327_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR6020252.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1472..4147
                     /gene="adhE"
                     /locus_tag="OO327_003903"
                     /gene_synonym="adhC"
     CDS             1472..4147
                     /gene="adhE"
                     /locus_tag="OO327_003903"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR6020253.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEDETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         1168 a         1021 c          960 g         1034 t
ORIGIN      
        1 aattcaatca aatagagatt ggctaagcat gtctatcttt tctgattaca gttccagtag
       61 tgaaatgcac aataatttaa caattgatta ttatttagca ctttcctcta ccaaaggcag
      121 tggaattact aatatcattt caattattct tcagcaagcc caggattatg atgtggcgaa
      181 aataacatga tggtgaggag aagcagttaa ggaaatcctt ataactaact gtaatttgag
      241 aagaatattt ttacctattc cttttgcttt tgataactgc gcgggaacaa agtaaaaata
      301 atgacgaagt aaacggagca tggcagctcc gtttcatttg aaaggaatta attaagcagg
      361 ccggggaaaa tccccttaat accagtaacg ataaattcaa tccccaatgc catcagcaat
      421 agccccataa tacgcgtaat cacgttgatg ccggtctggc gtaaaacccg taccagccac
      481 ggtgccatgc ggaacaatcc ccagcaacat aaagcgaaca atgcaatagc cacaaagaaa
      541 ccaaacagat agctaatgct gtgataacgc gtaccccaga cgatggtaga actgatcgcc
      601 cctggccccg ccatcaacgg caacgccaat ggcaccacac caatactttc acgtaccgcg
      661 gtttctgatt tttcttgctt gttctgttta tcctcgccaa gcttgccgct gatcatcgac
      721 atcgctatcg tcaccaccag gatgcccccg gcgatacgga acgaatcaat tgatatacca
      781 aaaagttgta gaatcgtgtc gccgagaaaa agcgagatcc acaagataat ggccacagac
      841 aggttggctg taaggttagt tttgtttcgc gctgccgctg tctgataact ggtcatgctg
      901 ataaagacgg gaataatccc taccgggttg accagcgcaa ataacccgat gaaaaatttg
      961 aagtaaacgg gaaaatcaaa aagggtctga atcacggttc gctccgaagc aaaagccgga
     1021 taatgttagc cataaataag gttgaaaaga cgcgctgaca atacgccttt tgacagcatt
     1081 tttcacctcc taactactta aaattgctat cattcgttat tgttatctag ttgtgcaaaa
     1141 catgctaatg tagccaccaa atcatactac aatttattaa ctgttagcta taatggcgaa
     1201 aagcgatgct gaaaggtgtc agctttgcaa aaatttgatt tggatcacgt aatcagtacc
     1261 cagaagtgag taatcttgct tacgccacct ggaagtgacg cattagagat aataactcta
     1321 atgtttaaac tcttttagta aatcacagtg agtgtgagcg cgagtaagct tttgattttc
     1381 atcggttaag caaatcatca ccgcactgac tatactctcg tattcgagca gatgatttac
     1441 taaaaaagtt taacattatc aggagagcat tatggctgtt actaatgtcg ctgaacttaa
     1501 cgcactcgta gagcgtgtaa aaaaagccca gcgtgaatat gccagtttca ctcaagagca
     1561 agtagacaaa atcttccgcg ccgccgctct ggctgctgca gatgctcgaa tcccactcgc
     1621 gaaaatggcc gttgccgaat ccggcatggg tatcgtcgaa gataaagtga tcaaaaacca
     1681 ctttgcttct gaatatatct acaacgccta taaagatgaa aaaacctgtg gtgttctgtc
     1741 tgaagacgac acttttggta ccatcactat cgctgaacca atcggtatta tttgcggtat
     1801 cgttccgacc actaacccga cttcaactgc tatcttcaaa tcgctgatca gcctgaagac
     1861 ccgtaacgcc attatcttct ccccgcaccc gcgtgcaaaa gatgcaacca acaaagcggc
     1921 tgatatcgtt ctgcaggctg ctatcgctgc cggtgctccg aaagatctga tcggctggat
     1981 cgatcaacct tctgttgaac tgtctaacgc actgatgcac cacccagaca tcaacctgat
     2041 cctcgcgact ggtggtccgg gcatggttaa agccgcatac agctccggta aaccagctat
     2101 cggtgtaggc gcgggtaaca ctccagttgt tattgatgaa actgctgata tcaaacgtgc
     2161 agttgcatct gtactgatgt ccaaaacctt cgacaacggc gtaatctgtg cttctgaaca
     2221 gtctgttgtt gttgttgact ctgtttatga cgcagtacgt gaacgtttcg caacccacgg
     2281 cggctatctg ttgcagggta aagagctgaa agctgttcag gatgttatcc tgaaaaatgg
     2341 tgcgctgaac gcggctatcg ttggtcagcc agcctataaa attgctgaac tggcaggctt
     2401 ctctgtacca gaaaacacca agattctgat cggtgaagtg accgttgttg atgaaagcga
     2461 accgttcgca catgaaaaac tgtccccgac tctggcaatg taccgcgcta aagatttcga
     2521 agacgcggta gaaaaagcag agaaactggt tgctatgggc ggtatcggtc atacctcttg
     2581 cctgtacact gaccaggata accaaccggc tcgcgtttct tacttcggtc agaaaatgaa
     2641 aacagctcgt atcctgatta acaccccggc ttctcagggt ggtatcggtg acctgtataa
     2701 cttcaaactc gcaccttccc tgactctggg ttgtggttcc tggggtggta actccatctc
     2761 tgaaaacgtt ggtccgaaac acctgatcaa caagaaaacc gttgctaagc gagctgaaaa
     2821 catgttgtgg cacaaacttc cgaaatctat ctacttccgc cgtggctccc tgccaatcgc
     2881 gctggatgaa gtgattactg atggccacaa acgtgcgctc atcgtgactg accgcttcct
     2941 gttcaacaat ggttatgctg atcagatcac ttccgtactg aaagcagcag gcgttgaaac
     3001 tgaagtcttc ttcgaagtag aagcggaccc gaccctgagc atcgttcgta aaggtgcaga
     3061 actggcaaac tccttcaaac cagacgtgat tatcgcgctg ggtggtggtt ccccgatgga
     3121 cgccgcgaag atcatgtggg ttatgtacga acatccggaa actcacttcg aagagctggc
     3181 gctgcgcttt atggatatcc gtaaacgtat ctacaagttc ccgaaaatgg gcgtgaaagc
     3241 gaaaatgatc gctgtcacca ccacttctgg tacaggttct gaagtcactc cgtttgcggt
     3301 tgtaactgac gacgctactg gtcagaaata cccgctggcg gactatgcgc tgaccccgga
     3361 tatggcgatt gtcgacgcca acctggttat ggacatgccg aagtccctgt gtgctttcgg
     3421 tggtctggac gcagtaactc acgccatgga agcttatgtt tctgtactgg catctgagtt
     3481 ctctgatggt caggctctgc aggcactgaa actgctgaaa gaatatctgc cagcgtccta
     3541 ccacgaaggg tctaaaaatc cggtagcgcg tgaacgtgtt cacagtgcag caactatcgc
     3601 gggtatcgca tttgcgaacg ccttcctggg tgtatgtcac tcaatggcgc acaaactggg
     3661 ttcccagttc catattccgc acggtctggc aaacgccctg ctgatttgta acgttattcg
     3721 ctacaatgcg aacgacaacc cgaccaagca gactgcattc agccagtatg accgtccgca
     3781 ggctcgccgt cgttatgctg aaattgccga ccacctgggt ctgagcgcac cgggcgaccg
     3841 tactgctgct aagatcgaga aactgctggc atggctggaa acgctgaaag ctgaactggg
     3901 tattccgaaa tctatccgtg aagctggcgt tcaggaagca gacttcctgg cgaacgtgga
     3961 taaactgtct gaagatgcat tcgatgacca gtgcaccggc gctaacccgc gttacccgct
     4021 gatctccgag ctgaaacaga ttctgctgga tacctactac ggtcgtgatt atgtagaaga
     4081 tgaaactgca gcgaaaaaag aagccgctcc ggctaaagct gagaaaaaag cgaaaaaatc
     4141 cgcttaatca gtagcgctgt ctggcaatat aaacggcccc ttc
//
LOCUS       DAJTSI010000232         4182 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000232 DAJTSI010000000
VERSION     DAJTSI010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.236"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..186)
                     /locus_tag="OO327_003904"
     CDS             complement(<1..186)
                     /locus_tag="OO327_003904"
                     /inference="COORDINATES: protein motif:HMM:NF017243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HCR6020254.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKK"
     gene            complement(327..2360)
                     /gene="metG"
                     /locus_tag="OO327_003905"
     CDS             complement(327..2360)
                     /gene="metG"
                     /locus_tag="OO327_003905"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR6020255.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2492..3601
                     /gene="apbC"
                     /locus_tag="OO327_003906"
     CDS             2492..3601
                     /gene="apbC"
                     /locus_tag="OO327_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR6020256.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3863..4144
                     /locus_tag="OO327_003907"
     CDS             3863..4144
                     /locus_tag="OO327_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCR6020257.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1016 a         1043 c         1062 g         1061 t
ORIGIN      
        1 cttcttcacc ttctccgcaa tcagcttcag ttccgctttt tccgccgccg cagcatctga
       61 aaaacttttt atctgacttt gccctttggt gccaactttt ccccaactga tatgcaactc
      121 gtttccctgt tgctccaccg tccagaattt atgtgatttt tcgtcctgat agataaagtg
      181 tctcattctg catccctata attttatcca gttgaatgca gatgctacca gtatttatac
      241 gggttagaga gagacaaaat cgcaagaaaa caactttagg aatttataaa gaaaaggcgc
      301 tgtcgatgca gcgccttgaa gggggattat ttcacctgat gacccggttt agcaccggca
      361 tccgggctta gcaggaaaat atctttcccg ccaggaccgg cagccatcac catgccttca
      421 gagataccga agcgcatttt acgtggtgcc aggttagcca ccataatggt gtgacgacca
      481 atcagtgcct gcggatccgg gtaagcagaa cgaataccgg agaagacatt gcgtttttca
      541 ccgccgagat ccagcgtcag gcgcagcagt ttgtcagaac cttcaacaaa ctctgcgttt
      601 tcgatcagcg ccacgcgcag gtcaactttg gcgaagtcgt caaaggtgat ggtttcctga
      661 atcggatcat ctgccagtgg gccagtaact ggcgcggtag tggctttcac ttcttcttta
      721 gaggcttcca ccagagcttc aacctgtttc atatcgatgc ggttatacag cgccttgaac
      781 ggattcactt tgtggcccag cagcggttgc tggataccat cccaggtcag ttccgtattg
      841 aggaaggctt ctgcacgctc ggtcagtttc ggcagtacag gcttcaggta agtcatcagt
      901 acgcggaaca ggttgatgcc cattgagcaa atcgcctgca ggtcagcatc gcgaccttcc
      961 tgttttgcga ctacccacgg agcctgctcg tcgacatagc ggttagccag gtcagccagt
     1021 gccatgattt cgcgcactgc tttaccaaat tcacggcttt cccacgcttc accaatcact
     1081 tcagcggcat cggtaaaggt tttgtacagc tcagggtcag ccagctcgct tgccagcaca
     1141 ccgtcaaaac gcttgttgat aaagcccgca ttacgggagg ccaggttaac cactttgttc
     1201 acgatatcgg cattcacacg ctgaacgaaa tcttccaggt tgagatcgat atcatcaatg
     1261 cgcgaagaga gtttcgcagt gtagtagtaa cgcaggctgt cagcgtcaaa atgattcagc
     1321 caggtgctgg ctttaataaa ggtgccgcga gacttggaca tcttcgcgcc gttcaccgtc
     1381 acatagccat gaacaaacag gttggtcggc ttgcggaagt tgctgccttc cagcatggca
     1441 ggccagaaca ggctgtggaa gtaaacgata tctttaccga tgaagtggta cagctcggcg
     1501 gtggagtctt tcttccagta ttcatcgaag cttacgctgt cgccgcgctt gtcgcacaga
     1561 ttcttgaaag aacccatgta gccaatcggt gcgtccagcc agacgtagaa atatttgccc
     1621 ggtgcgttcg gaatttcaaa accaaagtat ggcgcgtcgc gggagatatc ccactgttgc
     1681 aggccggatt caaaccactc ctgcattttg tttgccacct gctcctgcaa cgcaccgctg
     1741 cgggtccatg cctgcagcat ttcgctgaaa gagggcagat caaagaagaa gtgttcagaa
     1801 tcacgcatca ctggtgtagc accagaaacc accgatttcg gctcgatcaa ttcagtcggg
     1861 ctgtaggtcg cgccgcagac ttcgcagtta tcgccgtatt gatccgggga tttacatttc
     1921 gggcaggtgc ctttcacaaa acggtccggc aggaacatgc ctttttccgg atcgtacagc
     1981 tgagagatgg tgcggttttt aataaaaccg ttttctttca ggcgagagta gataagttct
     2041 gacaactggc ggttctcttc gctgtgcgtc gagtgatagt tgtcatagct gatgttaaag
     2101 cctgcgaaat cagtctgatg ctcctgactc atttcgccaa tcatctgctc cggggtgata
     2161 ccgagctgct gagctttcag catgatcggt gtaccgtggg catcgtcggc gcagatgaag
     2221 ttgacctcgt ggccgcgcat tcgctggtaa cggacccaga catcagcctg gatgtgctcc
     2281 agcatatggc cgaggtggat tgagccgtta gcgtacggca gtgcgcacgt caccagaatt
     2341 ttcttcgcga cttgagtcat agtaggcatt acttcttaat agtgaaaagg ggttagatag
     2401 taccaaatgg gaaaatgtta agtaagtact tctctatatt cttcaggtaa actatcagct
     2461 gatggtttat ctatacaaaa aaggagtcgg gatgaacgaa caatcccagg ccaaatcacc
     2521 agaagccttg cgcgcaatgg tcgccgggac actggccaat tttcagcacc caaccctgaa
     2581 gcataacctc accacgctga aagcgttgca ccatgtcgcc tggatggacg acacgttgca
     2641 tgtggaactg gttatgccgt ttgtctggca tagcgcgttc gaagagttaa aagagcaatg
     2701 tagcgccgaa ctgctgcgca tcaccggggc gaaagctatc gactggaaac tgtcgcataa
     2761 catagccact ctgaaacgcg tgaagaatca gccgggaatt aacggtgtga agaacattat
     2821 cgcggtgagt tccggtaaag gcggcgtggg taaatcgtcc acggcggtaa acctggcgct
     2881 ggcactggcg gcggaagggg cgaaagtcgg cattctggac gccgatatct acggcccatc
     2941 aattcccacc atgctgggcg cagaaaatca gcgtccaact tcgccggacg gtacccacat
     3001 ggcaccgatt atgtctcatg gcctggcaac caattcaatt ggttatctgg tcactgacga
     3061 caatgcgatg gtgtggcgtg gaccgatggc gggcaaggcg ttaatgcaga tgttgcagga
     3121 aaccttgtgg ccggatctcg actatctggt gcttgatatg ccgccaggca ccggtgacat
     3181 tcaattgacg ctggcgcaaa acattccagt aactggtgca gtcgtggtca ccaccccgca
     3241 ggacatcgcg ttgatcgatg cgaaaaaagg cattgtgatg ttcgagaaag tcgaggttcc
     3301 ggtgctcggt attgtcgaga acatgagtgt gcatatttgc agtaactgtg gtcaccatga
     3361 accgattttc ggtaccggtg gtgcagagaa actggctgaa aaataccata cacagctgct
     3421 ggggcagatg ccgttacaca tctccttacg tgaggatctc gataaaggca cgccaacggt
     3481 tatcagccgt ccggagagcg aatttaccgc tatctaccgc cagctggctg accgcgttgc
     3541 agctcagctc tactggcagg gtgaagtcat tcctggcgag atttccttcc gcgcggtcta
     3601 acgcctatta accattctgg ttattcttca tacaccgcag agatgttata ttgatgcggt
     3661 gtatttgttt ttgccgcatt aatgaacact tatttaatac tgcgaatatt ctgcggacct
     3721 tcgtccgaaa ctctacaaag tcgagtgatc gtacaggcaa tgcttcatta tcatccttcg
     3781 tgctgtgtag tggatgtaca gctatagttt gttatgcact cattctatcc aggcataacc
     3841 tttactacag ggataacgtt caatgaataa gtattggctg tcaggaatta tttttcttgc
     3901 ctatggattg gcttctcccg cagtctcttc agagactgcc acattaacga ttaatggcag
     3961 gatcagtccc ccaacatgca gtatggctat ggttaatagt caaccacaac agcattgtgg
     4021 tcagttaacg tataatgttg acacccgtca tcaggtttct tcgcccgtca aaggtgttac
     4081 gacagaagtg gttgttgcag gtagcgatag caaacgtcga atcgtgttaa atcgctacga
     4141 ttaatttggc aacctgattt taattgcatt tagaaagacg aa
//
LOCUS       DAJTSI010000233         4169 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000233 DAJTSI010000000
VERSION     DAJTSI010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.237"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..3298
                     /gene="narZ"
                     /locus_tag="OO327_003908"
                     /pseudo
     CDS             1..3298
                     /gene="narZ"
                     /locus_tag="OO327_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            3295..>4169
                     /gene="narH"
                     /locus_tag="OO327_003909"
     CDS             3295..>4169
                     /gene="narH"
                     /locus_tag="OO327_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR6020258.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDL"
BASE COUNT         1016 a         1143 c         1143 g          867 t
ORIGIN      
        1 ctacaaacaa gtgcgtgggc gcggcgggtt tatccgctcc aactggcagg aactaaacca
       61 gctgattgcc gccgctaacg tctggaccat caaaacctac ggcccggatc gcgttgccgg
      121 tttctcgccg atcccggcga tgtcgatggt ttcttacgcc gccggaacgc gttatctgtc
      181 gctgcttggc ggcacctgtt aaagtttcta cgactggtat tgcgacctgc cgcccgcctc
      241 gccgatgacc tggggcgagc aaaccgacgt accggaatct gccgactggt ataactccag
      301 ctacatcatc gcttgggggt ctaacgtacc gcagacacgt acgccggacg cccacttctt
      361 taccgaagta cgctacaaag gcactaaaac catcgccatt acccctgact actctgaagt
      421 ggccaaattg tgcgaccagt ggctggcacc gaaacaaggc actgatagcg ccctggcgat
      481 ggcaatgggg catgtgattt taaaagagtt tcatctcgat aatcccagcg actactttat
      541 caactactgc cgccgctaca gcgacatgcc gatgctggta atgctggagc ctcgcgacga
      601 tggtagctac gttcccgggc gcatgatccg cgcatctgac ctggtggatg gactgggcga
      661 aagcaacaat ccgcagtgga aaaccgtagc agttaatacc gcaggtgaat tggtagtgcc
      721 gaacggttcg attggtttcc gctggggaga aaaaggcaaa tggaatctgg aatccattgc
      781 cgccggtacg gaaaccgaat tgtcgttaac cctgctcggt caacatgacg ctgttgcagg
      841 cgtggccttc ccctactttg gcggcattga aaatccgcat tttcgcagcg taaaacacaa
      901 tccggtgctg gtgcgccaat tgcccgttaa aaacctgacg ttagtcgatg gcaacacctg
      961 tccggtggtc agcgtttatg atttggtact ggcgaattac ggcctcgatc gcgggctgga
     1021 agatgaaaac agcgcgaaag attacgctga aatcaaaccg tacaccccag cctggggtga
     1081 gcaaattacc ggcgtaccgc gccagtatat tgaaaccatc gcccgcgaat ttgccgatac
     1141 cgcccataaa acgcatgggc gctcaatgat tatcctcggc gcaggtgtta accactggta
     1201 tcacatggac atgaactacc gcgggatgat caatatgctg atcttctgcg gttgtgtcgg
     1261 gcaaagcggt ggcggctggg cacactatgt cggccaggaa aaactgcgcc cacaaaccgg
     1321 ctggttgcca ctggcctttg cgctcgactg gaaccgacca ccgcgccaga tgaacagcac
     1381 ctcgtttttc tacaatcatt ccagccaatg gcgctatgaa aaagtctctg ctcaggagtt
     1441 actttcaccg ctcgccgatg ccagtaagta cagcggtcat ctgattgatt tcaacgttcg
     1501 cgccgaacgt atgggctggc taccttctgc gccgcagctg gggcgtaacc cgctcgggat
     1561 taaagctgaa gccgacaagg ccggattatc ccccacagaa tttaccgccc aggcgctgaa
     1621 atcgggcgat ttacgtatgg cctgcgaaca accagatagc ggcagcaatc atccgcgtaa
     1681 tttgtttgtc tggcgctcta acctgcttga ctcctccggc aaaggccacg agtatataca
     1741 gaagtatctg ctggggaccg aaagcgggat tcagggcgag gaactcggtg ccagcgacgg
     1801 gatcaaaccg gaagaagtcg agtggcaaac tgcggcgatt gaaggcaagc tcgacctgct
     1861 ggtgacgctc gacttccgca tgtccagtac ctgcctgttc tccgatatcg ttctgcccac
     1921 cgccacctgg tatgaaaaag acgatatgaa cacctcggat atgcatccat ttattcatcc
     1981 gctttctgcg gcggtcgatc cggcctggga atcacgcagc gactgggaaa tctacaaagg
     2041 tattgccaaa gcattttcgc aagtgtgcgt gggccatctt ggcaaagaaa ccgacgtggt
     2101 attacaacca ctgctgcatg actctccggc agagctctca cagccgtgtg aagtgctcga
     2161 ctggcgcaaa ggcgaatgcg atctgatccc gggtaaaacc gcgccgaata ttgtggcggt
     2221 ggagcgcgac taccctgcta cgtatgaacg ctttacctcg ctcgggccat tgatggacaa
     2281 acttggcaat ggcggtaaag ggatttcgtg gaatacccag gatgaaatcg atttcctcgg
     2341 caaactcaat tacaccaagc gtgatggccc ggcacagggg cgtccgctga ttgacaccgc
     2401 cattgacgct tcagaagtga ttctggcact ggcaccggaa accaacggtc atgttgcagt
     2461 caaagcgtgg caggcgctgg gcgagatcac cgggcgcgaa catacccatc tggcgctgca
     2521 caaagaggac gagaagattc gctttcgcga tattcaggcg cagccgcgta aaattatctc
     2581 cagccccaca tggtccggtc tggaaagcga tcacgtatcc tataacgcgg gatacaccaa
     2641 cgttcatgag ttaattccgt ggcgcacgct gtcgggacgc cagcagctct atcaggatca
     2701 tccgtggatg cgtgcttttg gtgaaagcct ggtggcttat cgcccgccta tcgacacccg
     2761 tagcgtcagt gagatgcgcc agataccgcc aaacggcttc caggaaaaag cacttaactt
     2821 cctgacgccg caccagaaat ggggcattca ctcaacctac agtgaaaacc tgctaatgct
     2881 gacgctctct cgcggtggac cgattgtctg gatcagcgaa acagatgccc gagaactgac
     2941 cattgtcgat aacgactggg tggaagtatt taacgccaac ggcgcgctga ctgcccgcgc
     3001 ggtggtcagc caacgtgtac cgccgggcat gaccatgatg tatcacgccc aggaacgcat
     3061 tatgaatatt cctggttcgg aagtaactgg catgcgcggc ggcattcata actcggtcac
     3121 ccgcgtttgc ccgaaaccga cgcatatgat tggcggttac gcgcagctgg cctggggctt
     3181 taactactac ggcaccgtcg gttcgaaccg cgacgaattc atcatgatcc gcaagatgaa
     3241 gaacgttaac tggctggatg atgaaggtcg cgatcaggta caggaggcga aaaaatgaaa
     3301 atacgttcgc aagtcggcat ggtgcttaac ctcgataaat gtatcggctg ccatacctgt
     3361 tcggtgacct gtaacaacgt ctggaccgga cgcgaaggca tggagtacgc atggtttaac
     3421 aacgtcgaaa ccaaaccggg cattggttat ccgaaaaact gggaagatca ggaagagtgg
     3481 caaggcggct gggtccgcga tgtgaatggc aagatccgtc cgcgtctggg cagcaagatg
     3541 ggcgtaataa ccaaaatctt cgccaacccg gtggtgccgc agcagattga tgattactac
     3601 gaacctttca ccttcgacta cgaacatttg catagcacac cggaaggcaa acatatccct
     3661 actgctcgcc cgcgttcact gattgacggc aaacggatgg acaaagtgat ctggggccca
     3721 aactgggaag aactgctggg cggcgagttt gaaaaacgtg cccgcgaccg caacttcgag
     3781 gccatgcaaa aggagatgta cgggcagttt gaaaacacct tcatgatgta cctgccgcgc
     3841 ctgtgcgaac actgcctcaa tcccagctgc gtggcgacct gcccaagcgg cgctatctac
     3901 aaacgcgaag aagacggcat tgtgctgatt gatcaggata aatgccgtgg ctggcgtttg
     3961 tgcattagcg gttgtccgta caaaaaaatc tacttcaact ggaaaagcgg caagtcagaa
     4021 aaatgtatct tctgttatcc gcgaattgag tcaggccaac ccaccgtgtg ctcagaaacc
     4081 tgcgtgggtc gcatccggta tctgggcgtg ctgctttacg acgccgaccg catcgaggaa
     4141 gcgtcgagca ccgagcgcga agttgacct
//
LOCUS       DAJTSI010000234         4166 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000234 DAJTSI010000000
VERSION     DAJTSI010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.238"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..332)
                     /gene="flhe"
                     /locus_tag="OO327_003910"
     CDS             complement(<1..332)
                     /gene="flhe"
                     /locus_tag="OO327_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCR6020259.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGG"
     gene            complement(332..2409)
                     /gene="flhA"
                     /locus_tag="OO327_003911"
                     /pseudo
     CDS             complement(332..2409)
                     /gene="flhA"
                     /locus_tag="OO327_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2402..3549)
                     /gene="flhB"
                     /locus_tag="OO327_003912"
                     /pseudo
     CDS             complement(2402..3549)
                     /gene="flhB"
                     /locus_tag="OO327_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
     gene            3785..>4166
                     /locus_tag="OO327_003913"
     CDS             3785..>4166
                     /locus_tag="OO327_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR6020260.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNPPFG"
BASE COUNT         1037 a         1207 c         1021 g          901 t
ORIGIN      
        1 ccgcctggca cttcccagat aaatcgcaac ggttctgctg ccgctatacc ggaaaaggcc
       61 acggtggtac cgctctgccc ctctaattcg acacaacgag attgcgaaca caagcgaacc
      121 cgcagtcctg caggtgtcgg gccgataagc tgataacgcc acgccaccag cgtcatcaat
      181 cctgaagcag gttgtcgcgc agaaagaggt gcagacgaca tcgactcacc gcgatgattt
      241 agcgtaattc cgacactact tgcctgccac atcccctccc cggcggcttg caccagcagc
      301 ggaaacaata atatcgctaa taaggttctc atcatttacc gccaattgtc gccgtcatgc
      361 ggatatgtcg gttatcagac agttccagat tcgacaggac cactaactgc ggcaagctgc
      421 ggcgcaggaa gcgagacaat aatggtcgca gcgcgtggtt caccaacaat actggcggcg
      481 cacccagcat ctcctgacgg gatagagctt cctgagtttg cgccagtaaa cgatccgcca
      541 gccctggctc cagtcctccc ccgccctgca gcgcctgtag taacaaacgt tccagcggtg
      601 tatcgaggcc aataacatgg acttcatctt tgccaggaaa ccactgctgg gtaatcgccc
      661 gtcccaacgc cacgcgcacg acggcgctta attcatgtgg atcgctttgg atgggcgcat
      721 gttccgccag cgtttcgaga atggtgcgca tatcgcgaat cggtactttt tcatcgagga
      781 gattttgcag cactttatgc agtgtggtga gcgtgacgac gccaggaacg agatcttccg
      841 tcagctttgg catctcctgg gcgacgcgat ccaacagctg ttgcgcctcc tgacgaccaa
      901 acagctctgc ggcatgctgg ctaatgaggt ggttaagatg cgttgctacc accgtgctgg
      961 cctcaaccac tgtgtacccc tgaatctgcg cctgttcttt tagcgcactt tcaatccaga
     1021 tagcattcag gccaaatgcc ggatcgacgg tcgcctcacc aggtaacgtc ccggcagcgg
     1081 ttccagggtt aatcgccagc cagcgccccg gataagcatc accactgcca atctccacgc
     1141 ctttcatcaa aatgcgatag cgggcaggtt gcagatccat attgtcgcga atgtgcacca
     1201 ctggcggcag aaatcccatc tcctgggcaa atttcttgcg gatactgcgt atacggccca
     1261 acaactcacc atcctgctgg aaatcgacca tcgggatcag tcgataaccc acttccattc
     1321 ccagagaatc ttccagttgt acatcgttcc acgtcgcttc gacaacggta ttattctctg
     1381 ccatttttac cggtttgggt tcggcaggcg ctttttgttc gcgtccgcgt atccaccagg
     1441 ccagccccag caatccggca gtgaacagca aaaataccag gttcggcatt ccaggcacca
     1501 ggccgagtaa accgagcacg gcggcgctta acaacataac gcttgggtta ctgaaaagct
     1561 gattcaccat ctgctcgcca acatcctgat cggtgctgac acgcgtaacg atgccccccg
     1621 cggcggtaga aatcaccagc gccggaattt gtgccaccag accgtcgcca atggtcaata
     1681 gcgtataact ttccgccgcg tgtcccatgc tcatgccagt tgcagcacgc cgaccagcaa
     1741 cccgccgaca acgttaatga ccatgatgag gatcccggca atggcatcgc cgcgaacaaa
     1801 cttacttgcc ccgtccattg agccgtaaaa atcggcttcc tgagtcactt cggagcggcg
     1861 ttttttcgcc tcatcttcac caatcaatcc agcgttaagg tcggcgtcaa tcgccatctg
     1921 cttacccggc ataccatcga gaacaaagcg cgcacccact tctgctatac gcccggcacc
     1981 tttggtaatg accataaagt tgatgatcac gagaatgaca aacaccacga taccgatagc
     2041 gaaattgcca ccaacgagga agtgaccgaa cgcttcgacc accttcccag ccgccgccgc
     2101 gccggtatgc ccttccatca aaatgatacg ggttgaagcc acgttaagtg ccagacgcaa
     2161 caacatagta aacaacagaa tggtcggaaa cgcagcaaac tcaagcgtgc gctgggtaaa
     2221 catcgccacc agcaacacca tgatcgacaa ggcaatattg aaggtaaaca acaggtcgag
     2281 tatgaatgcg ggcagtggca gcaccatcat cgacaagatc aacaggatca aaatcggtcc
     2341 ggcaaggatc tgccattatg tcgatttcag gtttgcgggc aggcgcagca tcgcggccag
     2401 attactcatg ggtcggtttc tcgttaataa aatccagggc tgccggcacc ggaagatgag
     2461 taggtgtaca gggcgctgtc caccagccag acgccagcgt ttcagttgcc agacccaggc
     2521 cagcacttcc gccaccgcgg cgtacagttg acccgggatt tgttgaccaa tctccgcatg
     2581 tcgatacagc gctcgcgcca gcggcggcgc ttcaagcgtc gggacgttat tttcagcgcc
     2641 aatttcacga atgcgcagcg cgaccagccc tgcaccttta gcgaccactt tcggtgcgct
     2701 cattttgttt tcgtcatact gcaacgctac cgaatagtgg gtcgggttat tgacaatgac
     2761 atccgctttc ggcacatcgg ccatcatccg acgccgtgcg gcagctcgct gcatctgaca
     2821 gatccgtcct ttaacatggg ggtcgccttc gctttgtttg aactcatcac gaatatcctg
     2881 ccgcgacatt cgcagctttt tcaggtggct gaagatttgg aaaaagacgt caaatcccac
     2941 cattggaatg acaccaagca ccaccagcag tgcgcatagc cctaccaaat ccatcgcatt
     3001 acccatggcg gtaatcggtg actcggccat taagcgcatc atctgcggcc agtgatgcca
     3061 gagaaaaaac cccgtcacgc tgccaaccag gatggttttc aaaattgcct taagcaactc
     3121 cgcgctagtc tgtgccgaga atatccgttt aatgcccggt agcgggttga gtttggaaaa
     3181 cttcggctgc aaggatttgc cgctaaatac cagccccccc agcataaccg gagaaatgag
     3241 cgccaccagc accacgccgc taatcagcgg tagcagcgcc agcatggctt ctctgatcag
     3301 cagaataatt tgcccgagga tcagattcgg gtctttgata atactgtgat caaaatgcag
     3361 cccagcggag agcatgcccg acaatcgacg ggccagcgac acaccgccaa accagataac
     3421 actaacgccc accagcaaaa tcagcagtga ggtcagttca cgggaacgcg ggatttgccc
     3481 ctcttcccgc gctttttcta gtcggtgagg tgtgggggct tctgttttgt cgtcgctctc
     3541 gtcagacacg tcgccaatcc tggaaagagt attaagcgta aatgatgcca gagcgcaaag
     3601 cgttcaatgg tttaagtaag gggcaaaaca ggcgggattt agggcgttta tgcaaaaata
     3661 ggttcagttg cagaacgttt tgcgaaccat tttccagttc tgatatttag ccatttagcc
     3721 tatagaatcg cactccgcct ctctgttagt ttgctaataa ttaaattatt acaaaggtga
     3781 tcaaatggaa tggcaacaat tgcttaactg caaccgtcgg aaagataatc taaaaaaaac
     3841 caacatagca tttttacatc cccttgctag cggaagacaa gagatcgagc gggattacga
     3901 ccgattactt tttgctgcac caactcgtcg gcttgcggat aaaacacaag ttttcccgtt
     3961 agatcaaaat gacagcatac ggacgaggct gactcattct cacgaagtag caaactttgc
     4021 tcgcgggata ggaatgaggc tcgctttcga gatgaaggag cacatctttg gcgaaatacc
     4081 cacggggatt gtcgttgaac gcgacgttcc tgctctgtta gcagcgatcg gactggcaca
     4141 cgatctcggt aatccaccat ttgggc
//
LOCUS       DAJTSI010000235         4155 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000235 DAJTSI010000000
VERSION     DAJTSI010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.239"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(483..725)
                     /locus_tag="OO327_003914"
     CDS             complement(483..725)
                     /locus_tag="OO327_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020261.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
     gene            complement(738..1766)
                     /gene="spa40"
                     /locus_tag="OO327_003915"
                     /gene_synonym="spaS"
     CDS             complement(738..1766)
                     /gene="spa40"
                     /locus_tag="OO327_003915"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCR6020262.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSILVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            complement(1773..2543)
                     /gene="spa29"
                     /locus_tag="OO327_003916"
                     /gene_synonym="spaR"
     CDS             complement(1773..2543)
                     /gene="spa29"
                     /locus_tag="OO327_003916"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCR6020263.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            complement(2543..2803)
                     /gene="spaQ"
                     /locus_tag="OO327_003917"
     CDS             complement(2543..2803)
                     /gene="spaQ"
                     /locus_tag="OO327_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCR6020264.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            complement(2818..3468)
                     /gene="spa24"
                     /locus_tag="OO327_003918"
                     /gene_synonym="spaP"
     CDS             complement(2818..3468)
                     /gene="spa24"
                     /locus_tag="OO327_003918"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCR6020265.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            complement(3473..>4155)
                     /gene="spa33"
                     /locus_tag="OO327_003919"
                     /gene_synonym="spaO"
     CDS             complement(3473..>4155)
                     /gene="spa33"
                     /locus_tag="OO327_003919"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCR6020266.1"
                     /translation="SGFSTQESLLHLSKCVFIESSSVFSIPELSDKITFRITNEIQYA
                     TTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLATLKRIRTGD
                     IIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLALFNYDDINVK
                     VDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELVSIEDGYGIE
                     ISSWMVKE"
BASE COUNT         1583 a          751 c          593 g         1228 t
ORIGIN      
        1 aacatatcaa aaacaggcaa ttacacaatt taaattacag tctcaaaaca aacccaacgg
       61 tcaatatagt ttaccagcaa gcttactagc aaagtgctaa tcatttggta tctttcgtgc
      121 tattctcctt ttacgtaagt acttaagggg aacgtattta ctacttttaa agaaagctcc
      181 gttactatct gaaatgcaat gagataataa acgattactc tcggttagta tctcatgatc
      241 atttacagac tttgcataat cgcgaatact atataacaga gttttaacat tacaggatga
      301 atagtctctc ttatcaactc ctaataaaag aaggattatg gtcagtcgtt tttgctgtct
      361 tatcatatat tatttagtgc agttcctaac aaaaacataa caattttcta caattgatcc
      421 tgtgactaca taaatatctt caacaaaaat atttatatca gcaaaagagt tattatgaat
      481 gattataaat catcaggtgt tttttcaacg accaactcca gagcacaacg ataaatgtct
      541 tttctaacca ggtcacattc agatgaaatc tttctgagta aaacatttat tatatcctta
      601 ttgctaagga aagatattgg gttcaatacc tcattagcaa tggagttaat gatggaagat
      661 tctatttcaa attcagactg atcaatacct attttattac agaaattaac tcccatataa
      721 cacatctcct ttacttatta atgagtgttt tcaacctgct caagccaaac aataagacgt
      781 aggacttcat ccaagtgttc aaaatcaaca aaactatatt ttgtatgtgt tttatatagt
      841 tttctagcta atttcacatc acgcacagtc ggtataccaa cttcatttgc atattttctg
      901 acagccaagg cacactggtt agtttcaatg agagaaataa aaggtgcagg cgctatttct
      961 ggattaaaat aaataccaat tgcaatatga gtcgggttca ttaccactaa ttttgaatta
     1021 cgtatatcag atttagtctg ctctgaaaga atctcgatat gcaactcacg ccttctcgac
     1081 tttgtctcaa agtgtccctc ttgctctata tattctcttt ttatctcctg tttatccatc
     1141 atcatatctt tcatgtataa aatgaactca atcacaaagt caagaataat aacaagaata
     1201 gaaaatgcca agaaaaataa tattatatcc ttaaacagcc tcccccaaat aagatataag
     1261 ccatcaacac tagaaaacac ctgagaaaaa attatttttc ggtcattaat ccaaaagaaa
     1321 taggttgtta atgctagaat aataagaagc agaatgcttt tgaaaaattc ttttattgtc
     1381 tttatactaa atattttttt taacccttta acagggttta atactgaaaa atcaatcttg
     1441 atagctttag tcgcaagaac aaactttgtt tgaaccaatg ttggcaacac agccgaaaga
     1501 acacagaaaa aaagtgggaa gccaattatc ttaaaaaaga caataaccac tgcaaaaaag
     1561 tatttaccct cattaatttc gaagtcatta attattacat atctgtataa gagcattaca
     1621 tcacttaagg aaaagaatga tattatagta aatgtcccta ccagaataat aacaacagtc
     1681 gttaaatcct taaatttaaa tgactgtcct ttttttgcgg catcctttag ttttttaggt
     1741 gtcggctttt ctgttttatt tgccataata ttttatctaa caaatagatt tgtgaaaaat
     1801 ttatgttcac cgaggaaaaa ttgaacttta gaaaagtaaa tagtagaaga acagataaaa
     1861 ataataaata ttgcaagtaa acttttaata gttaatgata cggaaaaagc attcatctgc
     1921 ggagcaaatc tcgataatac accaagtaat acttctgata gtaacaatac tatcataaca
     1981 ggactggcta aaataacagc ctgacttgcc aataaagtca gaaatgttaa gatatttgag
     2041 acacggaaag aacattgaga aaataacggg catatattat aagacaattg tatggattct
     2101 aaaataaaga ccataccacc actgtataga aatacaactg cagaaaaaag attgaaaaat
     2161 tttgccaact cagacgtatc aacaccattg gcaggatcaa ttgaactact aagcgttgcc
     2221 ccacgctggt tgtcaataat gctaccaaca gcatgaaata tccaaaaggg aagagacaag
     2281 caaaaagaga gaaggaggcc aacaattatt tccttgaaca taaggaaata aacatgttca
     2341 aaaacagaag aacctatgtc taccttacca gaagtaatta atcctgatgc aacaagaaaa
     2401 atcacaggta ttctaataga tggagaaatt ataccgttat ttaaaaatgg aagaaagaaa
     2461 aataatggag ccaatctaaa aaaaacgccg ttcaggagta ttaaaaacac atgaatactt
     2521 tcgaaccagc ttgaaatgtc catcaaaccc cactcttaat taaaaacatt atttcatgac
     2581 aaaaagacaa taaaacctca ccataccatc cagaaagaag tagcaaagat attgagacac
     2641 ctataagctt tataccaaaa ggaagtgtct gctcttggag ttgagtcact gtctgtaaca
     2701 aaccaataga taatccaata accgtagcta tacctactgg ccataaggaa aagataagga
     2761 ttaaataaag agccttatta cccatataaa ctatatcaga cacattacct ccatcatcta
     2821 agcaggaata ttgatatatt gttcaatcaa ggccttagac aggatacccc acccatcaag
     2881 cgcaacaaat aatacaagtt ttattggtac tgatattgtg attggactca tcatcatcat
     2941 accaagtgca agtaaaatgc ttgaaataac aagatcaaca acaacaaatg gcaaatataa
     3001 ataaaaacct attttgaacg catctttgat ttcacttagc gcatatgcag gcaagaggga
     3061 aaacaatgaa taatcattat tctctgcact ctttaaatca gcattttctt cttcagacct
     3121 ttggaaaaaa cgtgctaact caagatctgt atgctttttc aagtattgct tatattccat
     3181 caagccagaa tcagaaaaac ggacaatatc agatattgta tcaaattttt gagggccatt
     3241 tagataattt tcataaccag cttcaataat gggtttcatc acgaacaaag ccataatcaa
     3301 tgcaatacca ttgagagtca tatttgatgg aacctgctgc aaaccaagtg catttcgtac
     3361 cattacaaaa acaatagaaa actttatata acaggtacca gcagcaacta aaaatggtaa
     3421 tagcgtaaaa aaagaaagtg tagcgatgag ggacatgtca ctcagcatga gattactcct
     3481 ttaccatcca agaactaatc tcgataccat aaccatcctc aatagaaaca agttccccat
     3541 gcccaaccaa agaaccattt actttaatat ttacatgttt aactatgtca tcgggaaact
     3601 tgaataattc gttttctaca tacattttta gttcattgat tgtcatattt ttttctaaaa
     3661 gaataaagtc cacttttaca ttgatatcat cataattgaa taatgccaaa gaaacttctg
     3721 tgtgttctga ctcaccatta ctttctgaca gattaatttt tgcctcatta ttatcattca
     3781 caatataatc cccaataata acttgattac acaataataa attataaagt ttctgaacta
     3841 tgattatatc accagtgcga attcttttta aagtagccag ccttacatta gatgaaccta
     3901 agcaaaactc taaaagatga tgcaatgagt caagagaaac ttgattacgt aataccggca
     3961 tcttgtcaaa ataaataata cctaaagaag aggaaaaaca gcataaatga ctcccagtag
     4021 ttgcatactg gatttcattc gtgatccgga aagtaatctt atcagacagt tctggaatcg
     4081 aaaatacaga tgacgactct ataaagacac attttgataa atgcagtaaa gattcttgtg
     4141 tagaaaatcc tgaaa
//
LOCUS       DAJTSI010000236         4119 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000236 DAJTSI010000000
VERSION     DAJTSI010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.240"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="OO327_003920"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="OO327_003920"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR6020267.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="OO327_003921"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="OO327_003921"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR6020268.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="OO327_003922"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="OO327_003922"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR6020269.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..>4119
                     /gene="argE"
                     /locus_tag="OO327_003923"
     CDS             3489..>4119
                     /gene="argE"
                     /locus_tag="OO327_003923"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR6020270.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMH"
BASE COUNT          950 a         1206 c         1002 g          961 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccagcacg cggaattaac gctatcgaac taatgcacg
//
LOCUS       DAJTSI010000237         4114 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000237 DAJTSI010000000
VERSION     DAJTSI010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.241"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..158)
                     /locus_tag="OO327_003924"
     CDS             complement(<1..158)
                     /locus_tag="OO327_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR6020271.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYL"
     gene            complement(308..469)
                     /locus_tag="OO327_003925"
     CDS             complement(308..469)
                     /locus_tag="OO327_003925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020272.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(466..654)
                     /locus_tag="OO327_003926"
     CDS             complement(466..654)
                     /locus_tag="OO327_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR6020273.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            914..1249
                     /gene="iraM"
                     /locus_tag="OO327_003927"
     CDS             914..1249
                     /gene="iraM"
                     /locus_tag="OO327_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR6020274.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1530..1638)
                     /locus_tag="OO327_003928"
                     /pseudo
     CDS             complement(1530..1638)
                     /locus_tag="OO327_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(2161..2237)
                     /locus_tag="OO327_003929"
     tRNA            complement(2161..2237)
                     /locus_tag="OO327_003929"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2201..2203),aa:Arg,seq:tct)
     gene            complement(2339..2414)
                     /locus_tag="OO327_003930"
     tRNA            complement(2339..2414)
                     /locus_tag="OO327_003930"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2379..2381),aa:Ile,seq:cat)
     gene            complement(2559..3380)
                     /locus_tag="OO327_003931"
     CDS             complement(2559..3380)
                     /locus_tag="OO327_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6020275.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3395..3751)
                     /locus_tag="OO327_003932"
     CDS             complement(3395..3751)
                     /locus_tag="OO327_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6020276.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3764..>4114)
                     /locus_tag="OO327_003933"
     CDS             complement(3764..>4114)
                     /locus_tag="OO327_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6020277.1"
                     /translation="PQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
BASE COUNT         1155 a         1118 c          793 g         1048 t
ORIGIN      
        1 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
       61 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      121 tatgacacac ctgttgtgat tttttccatc tggtccatac cccgtctccc gttatccgga
      181 agctgacaac aataaaaaag ccaccagtta actactgatg gctctgataa ctcatgcaag
      241 cgtctcagac gacccactga cactaccggt gagtttaacg ataccttcca tttgactggc
      301 tcacttttta tgatgatgcc ggtgcattta tctccagcac cagactttct atctcaacgc
      361 catacgttgc atttttggta atatccgtca gcgtcagtgc atttagtccc actgccagac
      421 tgtcttttat ggcctggaat gccgggccag tacgatgacg tagtatcact ccggctcagt
      481 tgcaccactg accaccacat caccttctgc tgcaatcgcc tgcatcaggg tataaggggt
      541 tatggccacc ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc
      601 cgcaagatct gacggcggcg ccgccctgac aacatcatag tgtttaaatg ccatggttct
      661 ttccaacatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa
      721 aaccatacct taaataaaaa cctgattatc aagcagatat gcatggataa actacaagac
      781 gagatataaa ccaccctgca tttaaataaa caataaacaa catcagaaaa ataattctgt
      841 tctatggttt aattcaaaaa tatcatttat acttttcaga acatcaccag caaggcataa
      901 acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat catgcggaat
      961 atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat cggatgcttt
     1021 cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca ataacaaagt
     1081 gctacctgta accatttaca acgtagtacc attcaataaa acattctgga atttaatcaa
     1141 aaacagccag gaatgcccta caaatacaga taacgtattg aatgaatgct ttaataaccg
     1201 ttgcactctg caaatatgcc cttatggact aaaacaacaa agtccataag gagtttactc
     1261 acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag tggcgctatg
     1321 tgccattcca tggtgccggg tgcctcccgg tgaattcagt accagcacct gaatccgcga
     1381 ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac catgccagtt
     1441 tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg tgaagtattt
     1501 aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt tttcagcaac
     1561 gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccggcac tgacattatc
     1621 cggctgctgc aatgccatta acaaaaacag taagcaatca ctttttaccg taacaggtga
     1681 taatccagat atgtatctac cccagatgag taatccgaag ttcatccata ccacaggtcc
     1741 tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca accatgaact
     1801 catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt cccgaaagac
     1861 gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt gctgcattga
     1921 ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata ttcactctac
     1981 ccacaccata tgaatgataa atacttaatg ctgaaaaaac cattagaccg tataacaaat
     2041 actcaatcaa tacttaacag aacttttatt tttgacaaat atataatatt ttcaacaata
     2101 tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga agaatgaata
     2161 tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc
     2221 ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc gccccccgca
     2281 ccaacaaccc tgttatcgtg tcgtctgctc ttcctgataa gctaatggcg gtttgtgatg
     2341 gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg ctctcaccac
     2401 tgagctaaag ggccgggagc agaataataa cggtccgtaa ttaatttcgc aataaaaaaa
     2461 cccgctcagc ggcgggttgt agaaactctt ctaacgccag gcataaaacg cccatcatta
     2521 tgacgaattt accacagatt ccggaaaaat caaccttgtt acctagttac cttttttaac
     2581 tgccgctcag cccatgcttc ttcaatatca aaccgggtca ccagcgcatc ataaaatttc
     2641 ttaactgttt tttcccatga cgcgcgtgtt atctggtttg tcacctcgca tatagcatta
     2701 aatgcctccg ttgatggtag tctttcatag ccacgaccac cacaacgctg gcagtctctg
     2761 ataacaggca taccacgttt taccgactct tcacgatgaa tggcgacacc acgcccacgg
     2821 caatccttac aggcggtgga aacctcaccc tttccgccac actccggaca ggcaactttt
     2881 accacctccc tgactttttt ccattcttcc cagtaaaacg gatacacacc tttcgtacac
     2941 tttgcccata ccggcggctt accatccgga tactggatct tgtttgtaaa aactatgctt
     3001 tcaataaatt tttccccata gcaacaaggg cactgctttt tactcgctgc gctgcgggca
     3061 taatcctcaa aagcgtacga agccataatg cgcatcacta ccggttttat ttctgccgga
     3121 agttttctca acgccgccac acgatcgcac cgactgagtg cataatctgc cagtaattct
     3181 gttgcccgcg ccctgtcatt catactgatg cccattttcc ccaggaacgc agaaaaaccc
     3241 atctcagccc gattctgtgt catgccctgc gcggccatca catcagtgat actcagcgca
     3301 tcttttgacg ttgaggccga tgcatcggtc aggccagggg attttgggga gtagtatttc
     3361 ggtaaatctt ccagtttcat tttttgacct gctcttaatg cattatgggg taaatcttca
     3421 cccccagacg tccaccagat actggctgac cacgaacgat attgatttca tcaaactgct
     3481 catcgtccat taacactccc gcatgcgtca gcgcatccag cggtgctttc aggatattgt
     3541 ccaggtcgcg acgacgctta tccggtggct ctgcaatcac ctttatcgcc agccttccgg
     3601 acaggcttaa tttcagccgc tgctggcgaa caataagcgc cacagcccgg cgataacgct
     3661 ttccctcctc cgagataaaa tatgtgctgc cacggcgtcg ccagtaagtg ttcaccgtcg
     3721 gcgggtaagg taaaaccaaa tctatgagca tcagtcacct cttttaccca agcacgccag
     3781 ttgcaaaggc gtgatcaaga aaacgaaaaa ttaaatcaac ctgagaacca tgcttttctt
     3841 cgaacgccag aggatccgca tgaagctcgt tgtgatgctc ccgacacagc ggtagcgtga
     3901 aaatatcgtg agatttggtc cccattccgc cctgaccatg accaatcagg tgatggggat
     3961 cgtcggctgg cttaccacaa cacgcacacg gctgtgtctt cacccagcgt ttgtatttct
     4021 cgttaaccca gcggcgacgt ttaggtcgtt tcatgaaaga ttccggagac tcaggatcaa
     4081 cggcaatgct gaccaccgtc ttttcctgtt gtgg
//
LOCUS       DAJTSI010000238         4042 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000238 DAJTSI010000000
VERSION     DAJTSI010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.243"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(886..1077)
                     /locus_tag="OO327_003934"
     CDS             complement(886..1077)
                     /locus_tag="OO327_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR6020278.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENL"
     gene            1142..1465
                     /locus_tag="OO327_003935"
     CDS             1142..1465
                     /locus_tag="OO327_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR6020279.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1407..2165
                     /locus_tag="OO327_003936"
                     /pseudo
     CDS             1407..2165
                     /locus_tag="OO327_003936"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2143..2673
                     /locus_tag="OO327_003937"
     CDS             2143..2673
                     /locus_tag="OO327_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR6020280.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2680..2913
                     /locus_tag="OO327_003938"
     CDS             2680..2913
                     /locus_tag="OO327_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR6020281.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
     gene            complement(3459..3851)
                     /locus_tag="OO327_003939"
     CDS             complement(3459..3851)
                     /locus_tag="OO327_003939"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020282.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          965 a          917 c         1208 g          952 t
ORIGIN      
        1 aaaaaaacca ccgctaccga cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgttcctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaacac ccgctgacaa gcccgtcaga ggttttcacc
      901 gtcatccccg aagcgagcga ggcaactgcg gtaaagctca tcggcgtggt cgtaaagcga
      961 ttcacagatg tctgcctgtt catccgcgtc cagttcgttg agcttctcca gcagcgttaa
     1021 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga
     1081 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgcgagag
     1141 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gctgtgaggg
     1201 taaacagctg gcggtatgga tgcggcgggt ctgcctgggt gagccggttg cccgttccgg
     1261 gaggctgccg acactggcac cgccgttact gcgtcagctg gccgccatcg ggaataacct
     1321 gaatcaggca gcgaggaagg tgaacagcgg gcagtggtct tccggtgacc gggttcaggt
     1381 ggtggccgca ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg ccgtcaggga
     1441 acaggggacg cgggatgata gttaagtttc atgccagggg aaaaggcggt ggcagtggtc
     1501 cggttgatta cctgctggga agggagcgta accgggaagg ggcaacggtg cttcagggta
     1561 atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa tacacgtccg
     1621 gtgttctctc gttcgcggag aaggagctgc cgccgggagg gcgtgaaaaa gtgatggcga
     1681 gctttgagcg tgtactgatg cccggtctcg aaaaaaatca gtacagcatt ctgtgggtgg
     1741 agcaccagga caaggggcgg cttgagctga attttgtcat tccgaacatg gagctacaga
     1801 ccggaaaacg cctccagccg tactacgacc gcgcagacag gccgagaatt gatgcctggc
     1861 agacgctggt gaatcaccat tacgggctgc atgacccgaa cgccccggag aaccgccgga
     1921 cgctgactct ccctgataac ctgccggaaa cgaaacaggc gcttgctgag agcattacac
     1981 ggtggataga cgctctttat catgtcgggg agataaaagg ccgtcaggat gtgattcagg
     2041 cgctcacaga ggcggggctt gaagtggtca gggtgacgcg aagcagtatc agcattgcag
     2101 acccgaacgg cgggaagaat atcagactga aaggagcatt ctatgagcaa tcttttacag
     2161 acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga gaaaatgctg
     2221 agcgacgagt tcaggaagct cgaagaatct gtaagcaagg ctgtgacatc aaacgagacg
     2281 aaaatcaaag acgctatagc cctgttcaca gcttcgacag aggaatcact gaaaaaacat
     2341 cgggaagggg tgaaagaggc gatgatgcag cacaggaagg acgtgttaaa gctggcaggg
     2401 aatacgggca tgatgttact ggggatggtc attttcctgt ttaccgtgag tggcgggacg
     2461 ctctggtatc ttggcgggat gatacaggcg aacctggaag aaatcaggaa gcagaacgag
     2521 agtctggaga agctgaacgc gaagacatgg ggcgtgcgtt atcacgaggg gagcaacgga
     2581 cgttttctgg tgttgcccga ggggatgaaa gccgaaacga actggacgat ggaaaacggg
     2641 aaacagaatg ccgtaaggct ggtacaggag tgacagagca tgacggagct ggaaagccat
     2701 ttgctgaacg cattagagca gctgcaacag gactatatgc agcggctgaa cgaatgggag
     2761 agcgccttcg tggaattgca gaaaatgttt tcgcttacgc aacgggacaa cgcgatgctg
     2821 aacgagcggg tcatgcagtt gagtcagcag gtgctgcact tgagcgagca gacagaacgc
     2881 ttgagccagt tatacagcga gaactggaga taagagagga acggttgata caggagcgcg
     2941 aacatgcgtt attcctggaa cgggaacgcc agccggaaat acaggaacgg acactggatg
     3001 gcccttcgct gggatggtaa tcagagacag gctaaaatgt tgtcagcgcg tttccggtgc
     3061 attttttggg ataatggttg gttcgggggc accgggaacg cgcttcagtc agaccttgct
     3121 gaaaacacag gaacgagtta aaacgcatac gggcgcgatt ctggtgtgtc ttttgtcgct
     3181 gtgttttgcc tctattgcca gatcgcgctc attcccctga acttctgttt tcccgctcag
     3241 gtccccgtct tcttcgcttc cgaaagcatg aataaaatcc gtttcagctg tctctggctg
     3301 gcaattcctg cgtgattcac atggctgcat agctatgcat gtggttaaaa tttaccgggt
     3361 gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     3421 gcgccgaacg cgtttcagcg gtgcgtacaa ttaagatatt acgttaaatt gtgagggaat
     3481 ggtccccata tactgacttt atcataggga tagggttggt agtgggtcga ttgggtttcg
     3541 gcattgtttc cgttaatgcc aattgcattt ttgtatttga tgcaacagaa ggcgtgagat
     3601 tctcctgggc ctggaaaggc taagtgttta gaattgatgg caaaatatct tccgtccgat
     3661 ttttgaagta gttgttgttt ggctgtctcc caaagggtgc tgttacaaac ttcttgaatt
     3721 tgtagttttt gcaggactct tattactgcg ttgtcatgtt ccaattcttt aggggagtgt
     3781 attattctct cttttaataa gctgtttacc acagggttta gttgttgtct aaagcacgca
     3841 gataatgcca ttgctacaca attttgttcc cgaggaagca tgtttggcgg ggtatgccgt
     3901 gtttgatgtg atatgtctat tctcatagaa ggtctctggc ttatagcatc aacaaatacg
     3961 gctataaaac aaaattttca tagagtgatt caagatcttc ttgagatcct tttttgcggc
     4021 gcgtaatctg ctgcctgcaa ac
//
LOCUS       DAJTSI010000239         4031 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000239 DAJTSI010000000
VERSION     DAJTSI010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.244"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1224
                     /locus_tag="OO327_003940"
     CDS             <1..1224
                     /locus_tag="OO327_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6020283.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1221..2186
                     /gene="yggF"
                     /locus_tag="OO327_003941"
     CDS             1221..2186
                     /gene="yggF"
                     /locus_tag="OO327_003941"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR6020284.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2208..2717
                     /gene="fumE"
                     /locus_tag="OO327_003942"
     CDS             2208..2717
                     /gene="fumE"
                     /locus_tag="OO327_003942"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCR6020285.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2714..3427
                     /locus_tag="OO327_003943"
     CDS             2714..3427
                     /locus_tag="OO327_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR6020286.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3399..>4031)
                     /locus_tag="OO327_003944"
     CDS             complement(3399..>4031)
                     /locus_tag="OO327_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6020287.1"
                     /translation="TDCLCDISLQLKQGEWLALTGDNGAGKSTLLRVMAGLLTPTAGT
                     VMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFGLKLQKCPADEITQRT
                     HAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLLDEPSRDFDENWLSVF
                     ESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVNPPDDIHP"
BASE COUNT          941 a          953 c         1180 g          957 t
ORIGIN      
        1 tttgaactgc cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc
       61 tgtttatcga cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat
      121 ttagaaaatc atccggtcat taccgggcat gaatgtgccg gggttattgt cgaagtgggt
      181 aaaaatctca ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg
      241 ttaccaagcg gatattcagc gggctacagc tacgaatatt ttggcggcaa tgccacttat
      301 atgattattc ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat
      361 tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat
      421 tatcacacca cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt
      481 gcactgctgg cgtgtgcggg tccgatgggc attggcgcta tcgattacgc cattaacggt
      541 ggcatacaac cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg
      601 cagaagctgc tgccggtgga tctggcggcc agtaaaggca ttgagctggt gtatgtgaat
      661 accaaaggga tgagcgatcc tatccagatg ctgcgggcgc tgacaggaga tgtcgggttc
      721 gatgacattt ttgtttatgc ggcggtgcct gctgtcgttg agatggctga cgaattactg
      781 gcggaagatg gctgtctgaa cttctttgcc gggctgacgg ataaaaactt caaagtgccg
      841 tttaatttct acaacgtcca ttacaacagc acgcacgtag tcggtacatc cggcggttca
      901 acggacgaca tgaaagaggc gattgccctt agcgccacag gacagttaca gccgtcgttt
      961 atggtgaccc atattggtgg cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat
     1021 atccctggcg gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc
     1081 gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag
     1141 gaaacgcacg gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt
     1201 gatatcgggg aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac
     1261 aggcagcgtt ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc
     1321 tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga cgagtggtta
     1381 tcggcgaagg agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag
     1441 gcgatgggcc agaagtcgat atcgcggttg atcccattga aggcacgcgg atggtggcga
     1501 tggggcagag caatgcgctg gcggtcatgg ctttcgctcc tcgagatagc ctgttacatg
     1561 cgcccgatat gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc
     1621 tgtctttgcc tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg
     1681 acaagctgcg catggttacg ctggataaac cgcgtctgag tgccgcgatt gaagaagcaa
     1741 cacagctggg cgtgaaggtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga
     1801 cctgctggca ggataatcct tacgatgtga tgtacaccat cggcggtgcg ccagaaggcg
     1861 tgatttctgc ctgtgccgtt aaagcgttag gcggggatat gcaggccgag ctgattgact
     1921 tttgtcaggc taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc
     1981 gttgtaaggc aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg
     2041 ggaacgatat cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc
     2101 aacagacggc gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt
     2161 gtaatataat agactccctg cattgatggt gatctatcgg aagaccaatg gcgacgctga
     2221 cagaagatga tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa
     2281 ctgcgcattc tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata
     2341 acgatgaaga gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg
     2401 acgatattga tgttgcgctg cgtttgattt atgcgctggg gaaaatggat aaatggctgt
     2461 atgccgatat cacgcacttt tcccagtact ggcattacct gaacgaacag gatgaaacgc
     2521 ccggatttgc cgatgatata acctgggatt ttatcagcaa tgtcaacagt ataactcgca
     2581 atgcgacgct ctacgatgcg ttaaaagcga tgaagttcgc cgatttcgcc gtctggtcag
     2641 aggcgcgttt tagcggaatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa
     2701 aggaattaac gccgtgaaaa ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta
     2761 ccatgatgaa gagatagaac gcgtgcataa actgttgctg cgtatgctgg cggccttaca
     2821 gacggtaaat ccgcagcggc gtacggtggt ttttctttgc gctccgccgg ggacgggtaa
     2881 atctacccta accaccttct gggaatatct cgcgcaacaa gacccggagc tgcccgctat
     2941 tcaggcgctc ccgatggatg gttttcacca ttacaatagc tggctggacg cgcatcaatt
     3001 gcgccccttc aaaggcgcac cagagacatt tgacgttgcg aaactggcgg aaaatctgcg
     3061 ccaggtggtg gaaggggatt gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt
     3121 tgaagatgcg ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct
     3181 ggacgacgag aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaacgc
     3241 gccagctgct gcgttgcggg agcgtctggt ggggcgcaag ctggcgggag gggtatcgct
     3301 ggcagatgca gaagcctttt atgaccgtac cgacgggccg aatgttcgcc gggggctgga
     3361 agagagtctg cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctggt
     3421 ggattaacgt tcctgattac accatcttcc agccgcacca cacgagagaa atgacgccgg
     3481 gtaaatgcgg cgtcatgact gattgctacc acgctcgtgc ctcgctggcg gcacttctcc
     3541 agccagcttt caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc
     3601 agtaatagtg gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca
     3661 ttatgcagat ccaacgggtg cgagttggca gtgtctgcta actgacaaca ttgcagggca
     3721 gcatgggtac gttgagtgat ttcatccgcc gggcattttt gcagtttcaa accaaatgcg
     3781 atctcgtcag cgacggtgct gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca
     3841 acctttgccg cgcgctggcg gtttttcagg tttttcatcg cctgttgttg cagcataacc
     3901 gtaccggctg tgggcgtaag aagacctgcc attactcgta acagcgtcga tttccctgcg
     3961 ccattgtcac cggtcagcgc caaccattca ccttgtttga gttgcagaga aatgtcgcaa
     4021 agacaatccg t
//
LOCUS       DAJTSI010000240         3800 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000240 DAJTSI010000000
VERSION     DAJTSI010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.245"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..911)
                     /locus_tag="OO327_003945"
     CDS             complement(<1..911)
                     /locus_tag="OO327_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6020288.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGII"
     gene            complement(935..1132)
                     /locus_tag="OO327_003946"
     CDS             complement(935..1132)
                     /locus_tag="OO327_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCR6020289.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            complement(1304..2224)
                     /gene="lpxL"
                     /locus_tag="OO327_003947"
     CDS             complement(1304..2224)
                     /gene="lpxL"
                     /locus_tag="OO327_003947"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR6020290.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            2449..3501
                     /locus_tag="OO327_003948"
     CDS             2449..3501
                     /locus_tag="OO327_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR6020291.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            complement(3543..>3800)
                     /locus_tag="OO327_003949"
     CDS             complement(3543..>3800)
                     /locus_tag="OO327_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR6020292.1"
                     /translation="FTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGG
                     KRAGFEAEGKIKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
BASE COUNT          974 a          936 c          903 g          987 t
ORIGIN      
        1 ttgatgatac ccctgaacaa gttgactgat agcggattac ttgatacaga agccctacca
       61 tattgccctg tgttcagttg ctgtactcga tagaatagtg actcactgat tattgcgggg
      121 tagtaaccct gaacttcttc acgtccagca ggtgtgaaac atccaatcac agcacgattt
      181 ctgagcagtt tctttacagc agtatcagac cacccctttt gtttgggtgt gtattgattc
      241 aatgacaagt gaccttcatc gtttaaagtt ctggtgatag cccaaaagct taaaccctgt
      301 aatctcaggt caaatgctcg tttaacaact tccactttat caggaatgat tgtgaatgtt
      361 cgacggtcat cattcagcct taaccatgca ggacaacgcc tagatacctt gataccttcg
      421 gagatgagtt cttttttacg gttccatgca gcttggacac gctcagactt tctcaaactt
      481 tcctcatgcg cccgctgcat tatcagaatg cttttaatca gggctaacgg gtcttttaaa
      541 ctttctcgtg tgtaatgttc tccatcgctc agtgtgacta catctacacc agcccttaag
      601 atacggcgta atagctcaga agcaatatca atatcctgac gtgacagacg gtctaaactc
      661 tcgattagta gcgtatcacc agctttaaca agccctttct cgatagcggt taaaaagtca
      721 cctagaccac ctttcaggtg cttaccacta aaagcacttt tccccaaatc tttaaagctc
      781 aaggatgaat ccaagtaaaa ctcagggttc ttatcaagcc agtcagtaac cagcttagat
      841 tgtcggcgta cagagtcccc tttcagttgt cgttcacttg aaaatctgat gtagctgatt
      901 gcccgtctca tattcaatac ctaaaatgat gtgtttagga acccattata ctgaagacaa
      961 taccggacca catattcagg gcggagtggc cggtaacacc aagctgctca acgtagaggg
     1021 gtaagaaggg cattaccaga ctgaaggcgg ccccggtaag aaaacagcct agccaggcga
     1081 cgatcaggtt tcgtttccag tttatagggg tgtcattttc acagggtgac atagcaatcc
     1141 gctgttggtg cgccaggcgc ggtgaacata agaagaaaag ataaacacac taattatgcg
     1201 cccgacttcc agggggcgca atccagagag cttttatcgc taaatcaggg ggatttgctg
     1261 tggtaatgcc ggatgccatt ctgaagcatc cggcatggga gatttaatag cgtgaaggaa
     1321 cgccttccgg gcgtgtttta aagcgacggt gtaaccacat atactgctct ggtgccatca
     1381 tgatgcattt ttcgaccact ttgttcatcc acgcggcggt agtttcggca tcatccagtg
     1441 gcggagaaca ctctggtggc agcataatca attgataccc tttgccatct ggcttacggc
     1501 gtggaacgaa gggcaccaga catgcgccgg acatccgtgc cagcatccag gttccggtcg
     1561 tggtcgcagc ctgctcaacg gcaaataacg ggacgaaaac gcttgagcgc gggccgtaat
     1621 catgatctgg tgcgtaccag accacttcgc cttttttcag ggctttaatc atgcctttta
     1681 aatctttgcg gtcgagcatc gatttatttg agcgcaaacg gccccaggtt tgtagccagt
     1741 caatcagtgg attatcgttc gggcggtaaa cgccaatccc cggttcctgc ataccaaact
     1801 gccgcgcgcc cagctccagt gtcagaaaat ggatgccaac taacaggatg ccgcgttttt
     1861 gcgcctgcac gtcacgaatg tgttccatgc cgatcacttc cgtccagcgg gcgattcggc
     1921 ggtccggcca gaaccacgcc ataccggttt ccatcaggcc catgccaacg gattcgaaat
     1981 tcttcaccac cattttacgg cgttcttgtt cgctcatttc cgggaagcac agttccaggt
     2041 tgcgatgcac aatttttgcg cgtcgtttca taaaacgtaa cgccagtttt cctaatccac
     2101 aaccgaggcg gtagataatc gggtagggca attgcacgac caaccaaagt acgccaatac
     2161 ccaaccaggt taaccaataa cgcggatgaa gcagtgcggt ggagaacttg ggtagattcg
     2221 tcatatcaat cctgtttttc aacctatacg ggcaattgta tgtattgtcg cattttttcg
     2281 cccgcaacca aaatttgtgg ctgaagactg ggcgaaattg ccgcgcttgt aaataacaaa
     2341 taatttttaa tgcgcaaatg tagcgtaaaa tgtgtggatg ttaattatcg ataattgcta
     2401 tatcatgccg cggattttta ctttcccatc tcgcaggaac cgtacaccat gccagtgtta
     2461 cacaaccgca tttccaacga cgcgttaaaa gccaaaatgt tggctgagag cgaaccgcga
     2521 accaccattt cgttttacaa gtatttccac atcgccgatc ctaaggcgac ccgtgacgct
     2581 ttatatcagc tgtttaccgc gctgaatgtt tttgggcgag tgtatctggc gcatgagggc
     2641 attaacgcgc aaatcagcgt acctgcgagc aatgttgaaa catttcgcgc gcagctctat
     2701 gccttcgacc cggctttaga gggtttacgc ctgaatatcg cgttggatga tgacgggaaa
     2761 tccttctggg tactgcgcat gaaggtacgc gatcgcattg ttgccgacgg tattgacgat
     2821 cctcactttg atgccagcaa tgttggtgag tatctgcaag cggcggaagt gaacgccatg
     2881 cttgacgatc ccgatgcact atttatcgac atgcgtaacc actatgagta tgaagtgggg
     2941 cactttgaaa acgcgctcga aattccggca gataccttcc gtgagcagct gccaaaagca
     3001 gtcgagatga tgcaggcaca taaagataaa aaaatcgtca tgtactgcac cggcggcatt
     3061 cgttgtgaaa aggccagtgc ctggatgaaa cataacggat tcaacaaagt ctggcatatc
     3121 gagggcggaa ttattgaata cgcccgtaag gcgcgcgagc agggcttgcc ggtgcgtttt
     3181 attggcaaaa attttgtttt tgacgagcgg atgggcgaac gtatatcgga tgagattatc
     3241 gcgcattgcc accagtgcgg cgcgccgtgc gacagccata ccaactgtaa aaatgatggc
     3301 tgccacctgc tgtttattca gtgtccagta tgcgcggaaa aatacaaagg ttgttgtagt
     3361 gagatttgct gcgaagaaag cgcgttaccg ccagaagaac agcgacgccg tcgggcagga
     3421 cgtgaaaatg gcaataagat ctttaataag tctcgtggac gtctgaatac aacactgggt
     3481 attcctgatc caacagaata aatatcattg ccggatgcgt gccatccggc aacatttcac
     3541 tcttacttct gctgtacgcc ttccactgaa ataatcagat ccacttcctg agaagctgga
     3601 cctaaatctg tcttgatatt gaagtctttg agcttaattt tgccttcggc ctcaaagcct
     3661 gcacgtttac cgccccatgg gtcgtcgccc tgaccaatta atttcgcttc cagcgtgacg
     3721 ggtttggtta cgccattcag cgtcagatcg ccggtaatat ccagttcgtc accgtctttc
     3781 ttcacgctgg tggaggtgaa
//
LOCUS       DAJTSI010000241         3784 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000241 DAJTSI010000000
VERSION     DAJTSI010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.246"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(69..2330)
                     /gene="katE"
                     /locus_tag="OO327_003950"
     CDS             complement(69..2330)
                     /gene="katE"
                     /locus_tag="OO327_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCR6020293.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            2513..2776
                     /gene="cedA"
                     /locus_tag="OO327_003951"
     CDS             2513..2776
                     /gene="cedA"
                     /locus_tag="OO327_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCR6020294.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            3053..3784
                     /locus_tag="OO327_003952"
                     /pseudo
     CDS             3053..3784
                     /locus_tag="OO327_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          969 a          830 c          925 g         1060 t
ORIGIN      
        1 gggtagttat cttgatgtgt aagtaaggtg gtaaggtcac tccccagggg acgtgacctt
       61 atccatattt acgccggaat tttgtcaatc ttaggaatgc gtgaccacac gcggtgttct
      121 gccatcagcg ttaacagttc atccataaaa ctaccgtcgg tgctgtcagc ttccacaatc
      181 ccttcttcac cctggtcagc taccttgatt gttgctttaa acttgcgcgc gtctcccgcc
      241 agcgcaatcg gtttaaggtg tttgtaggct tccatcagat agtagttggc atcgccgttg
      301 tcagcgatat ccgcgatatt gccgcaaggg acaatgaccg catcgaccgt cagcgaaggt
      361 gcaccggcaa aggtagcggc tataggcaac accgtaccgt catccgcagt cacttcaccc
      421 attcgggagt agagcagttt ggcatgaacg cctttggcct tcatggcctt gagaatggcc
      481 agaaggtctg ccgatctcac ttcatcatta agtaaaatcg ctaccacgcg acctttcaca
      541 tcaccgtcag gaatggcgta caaacttaag gatggatcct ttttcagacc gttgacgtcc
      601 ggaggtgggg tgatattcag ctggtcgtca gtcagttcga taccgagatt tttcgccacc
      661 gcctgggcca gagtgagatc aatatgcgcc agctggtcaa caacgcgctc acgaatatac
      721 ggacgaacga ctttgcttaa ctcaaaactg aaaccatcga caatatggcg ctgctcgaat
      781 ggcgtctgac ttagccagaa cagacgcgga tgggaataat attcgccaaa cgatgggctg
      841 cgctcgcgaa ctttattgcc ttccacgcgc tcttggtatg attcaaaacc gccgcgtttc
      901 ggccccggtg gtgtttcgcg cggccagtta tcgttgatcg agttcggttc ataattcgcc
      961 gggttagtgt cgatccccat acgatgcatg ccgtcacgct ggaaattatg gtaagggcag
     1021 gtcgggcggt taatcggaat ctcatggaaa ttcggcccac caagacgact gatttgtgta
     1081 tcggtatagg agaacaaacg tccctgcaac aggggatcgt tggtgaagtc cagaccagga
     1141 acaatatgac ctggatggaa tgctgcttgt tcgttttcgg caaagaagtt atccggattg
     1201 cgattgagca ccattttgcc gacacgctga acgggcacca gttcttccgg gatcagtttg
     1261 gttggatcga gaagatcgaa gtcgaacttg aattcatctt cttcaggaat caactggaag
     1321 cccagttcgt attccggaaa atcgcctgct tcaatggctt cccacaactc gcggcggtgg
     1381 aagtccgggt cacgtccggt gagtttttgt gcttcatccc aaacgagtga ggctttacct
     1441 gccagtggtt tccagtggaa acgtacaaac gttgccttcc cttcggcatt aatcaggcgg
     1501 aaggtgtgaa taccgaagcc ttccatggtg cggtaactgc gggggatgcc gcgatccgac
     1561 atcgcccaca tcacgttgtg cagagtttca ggttgcagag aaacataatc ccagaaagta
     1621 tcgtgggcac tttgcccttg tggaattgcc cagtgcggtt ctggttttac cgcatgaaca
     1681 aaatcgggga atttatgcgc atcctggata aagaagattg gcgtgttatt gccaacgagg
     1741 tcaaaaatac cctcttcagt atagaacttg gtggcaaaac cacggatatc acgcacggta
     1801 tcggcagagc cagcgccacc ctgaacggta gagaacctaa caaatactgg ggtgattttg
     1861 ttcggatctg agaggaaatc cgctttggtg atatcgctta agcttttata tggctggaaa
     1921 taaccgtgag ctgctgagcc gcgtgcatga acaatacgtt ccgggatgcg ctcatggtca
     1981 aagtgggtga ttttctcgcg cagaataaaa tcttccagca gcgttggacc acggttaccg
     2041 gcacgcagtg agttttgatc atcggcgatg cgcacgccct gattagtggt cagcgcataa
     2101 ttttcactgc ctttgcgtac gtcttccaga gaattaagtt tttcgttacg tgtatcaggg
     2161 gctttcaggc tcccaggggc ggtaggttgt gcacctggcg gtgttggttc agccgctgga
     2221 cgatgagatc cgtcctcagg tgccagtgag tccatccccg gtttcgcttc gctggaatcg
     2281 tgtagtggtg actggtgctg atgtgggttc ttttcgttat gttgcgacat tgaactcgtc
     2341 tccttaattt attactgaaa gggccgctgt tttggtcaaa aaactctcta actatagacc
     2401 accagccaga tcccgcttcg gagactattc tggaaacggt gttatgaaat acacgcaaag
     2461 cgggacgtac aggggctaaa tcggctaaac tacagttttt ctaattttta atatgcgttt
     2521 agtgaagcca gtaatgaaga aaccgctccg ccagcaaaac cgccagatta ttagctatgt
     2581 cccacgcacg gaacccgcgc cgccagaaca tgcgataaag atggattcgt ttcgtgatgt
     2641 ctggatgctg cgtggcaaat atgttgcgtt tgtactgatg ggagagtcat ttctgcgctc
     2701 accggcgttt actgtgcctg aatctgccca acgttgggca aatcagatcc gccaggaagg
     2761 ggaagtgact gagtaaggat tacatacctc ccggttttgc cgggagttac aggacctgaa
     2821 attctgcatt tcgttgctac attcattcaa accacctgta acaaaaagct gcctttgacg
     2881 agctattgct ataaataaga gatctttttt ctttaattat ttatttattt taaaatggtt
     2941 aagttttgct aatcatgatg agttattttt gatagttcat cctgtggcgt cgctcttaaa
     3001 cttatattgt aaatgactta gatttaaata agataataat tttgttaaca atatgaggcg
     3061 ggagatgttt tctacaaact taatacagag taattatggt gatttaaata ttaaaagcct
     3121 ggctttcgac tcttttaaag aaaggcttga gtctaccatg gcggcactca ctttctttat
     3181 atcaacgggt caatgtgact gcgatgaaat agcagagtcg aacttcaatc gtatgattgc
     3241 ctatatgtct aacattaact atgatgcctc aaagctagga gcgccagcac ttagttttga
     3301 tacgtatctg caggacaatg ttaaatatag agtgataatt aataatcttt atggcagtga
     3361 gtttcggata agagatatta atgagaattt tgttgggatg gattttacct gtgaattccg
     3421 acccgaaaaa atgacgaatt tgatcaatat aaaaaatagg ttggttattc attatttaag
     3481 gactatatac tatgaaaaaa attatataca tcctgccggg agcatttttg ctgcgattca
     3541 aaaaaaatga gagtctccta aagtttcctt cgataatatc aatgcttaat atcaatcttc
     3601 tttttaatcc actaaatctt ccgggaatgg gaagtggtgt gcttgaagat attatgagta
     3661 ttccagatag ttcacttaga aaacgtctcg ggtatgaggt tttaagcttc tcactacagg
     3721 cacattcatt gagccaggaa tgtattgata agttggacat ttttttcgct gacgatctgt
     3781 ttaa
//
LOCUS       DAJTSI010000242         3767 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000242 DAJTSI010000000
VERSION     DAJTSI010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.247"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(452..824)
                     /locus_tag="OO327_003953"
                     /pseudo
     CDS             complement(452..824)
                     /locus_tag="OO327_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1068..1187
                     /locus_tag="OO327_003954"
                     /pseudo
     CDS             1068..1187
                     /locus_tag="OO327_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            1437..2303
                     /gene="ospB"
                     /locus_tag="OO327_003955"
     CDS             1437..2303
                     /gene="ospB"
                     /locus_tag="OO327_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCR6020295.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            2632..3372
                     /gene="phoN2"
                     /locus_tag="OO327_003956"
     CDS             2632..3372
                     /gene="phoN2"
                     /locus_tag="OO327_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCR6020296.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAENSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
BASE COUNT         1150 a          744 c          759 g         1114 t
ORIGIN      
        1 ggggaaaaac gtgtattttt agtcattctg tttacctctt tctcagggcg tttagtctcc
       61 aggatttccg gggcggttca aaaaactctt cgttgattac tgtggactta ctgttggcat
      121 taccgaccct gagaccggag aaataagaac cgcttagatc atcgtggctg ttctcggggt
      181 atcaagttac acatgggcag aggctaccca aggtctacta ttatcgcctg aaagcgctga
      241 tggaacagtg ctatcaggga catgctgatg aaaataccta aataatcagc cagcactcta
      301 tctttccaaa tccacagcat agcaaagaga gcaaaagagc ctgtaaattc agaaattaag
      361 gaaacgcctc ttggtgtaca taatctatct tatgttaaat gaaatctaat ttcttgatta
      421 tatgctggat cgttcgtggg tggctttgcc cctatatttc cagacacctg ttatcattgg
      481 cccgctgccg cagatattcc cgtggcgagc gataatccag cgcactatgc ggatgccatt
      541 cgttataatg ctcgaatgcc tctgcaaggt tctttgctgc cgttaacccg tcaggtttgg
      601 gcatgatact gatgtagtca cactttattg ttttcacgaa gctctttgct attctgttac
      661 tctccggact ccgcaccgcc gtgttcttcg gttcaacaaa gtcccaacat ccgggtgaac
      721 tggcgtgttt cattagctcg gtagcatgaa ccattatccg tcagtcactt cactggagac
      781 gccggaagct cgttgccgaa gcggtgttcc accgctccca gcatgtcgcc aacgggagag
      841 catctacggt ctgattgaac tgtttattca aattgtccac cgtctgtcag tacgctctga
      901 acgtcggctg gttaaaacac tgctcgctga ttttcagagg gttcacggta aaacagcact
      961 tttattccgg attgcggaag cggctctgaa taatccggat gggctggtta aagaggttgt
     1021 gtaccatctg catattgttg aaccagatcg cagcggtaag agaagttcat catacttggc
     1081 gcagttacgc gatgtttctg ctagaggtga tgccgtaaag aacggcagaa cattaccgga
     1141 acaagatagc ggtctacctg cactggtatc agacccgggg cttccccgga tgatctccac
     1201 ggtactttag gtactgtgtc tatattatgt aggtaggatc aattaacttt gtagttgaaa
     1261 ttctttcatt gaaataatga ataaaaaaga caaaccgcat taaacaatgc cagaatataa
     1321 ttgcattttt gttccgtttt tttaaatcat agtatacaaa gaagcttgca tactatatga
     1381 aaatataaca ccctttacta tgatgtttat aaaacaatat atggagtcat gtaggtatga
     1441 atttagatgg tgttagacca tactgtagaa tagtcaataa aaagaatgaa agcatatcag
     1501 atattgcatt tgcacatata ataaaaaggg taaaaaattc atcatgtact cacccaaaag
     1561 cagcattggt ttttttagga gagaaaggtt tttgtgatag caatgatgtt ctatctatta
     1621 tgggacaaca aataccaaga gtatttaaga acaagatgtt atatgattat gtttttaaaa
     1681 atgaaaaaag taaaaatgat tttctaaaaa tggctgaatc atggctacca cagagtgaac
     1741 caatagtaat aaataatgat gatgacgcat tgaatgctgc tgcttatttt tctgtaaaaa
     1801 aagcgaaaat aaaaacagta aacgatactg attttaaaga gtataataag gtttatattc
     1861 ttgggcacgg tagtcctggt tctcatcaat taggccttgg ttcggaactt attgatgtac
     1921 aaacaatcat ttcaagaatg aaagactgtg gtattctaaa tgtgaaagat atccgtttta
     1981 cttcatgcgg ctccgctgat aaagtggctc ctaaaaattt taacaatgcc cctgctgaaa
     2041 gtctttcttg tatccttaac tctctgcctt tttttaagga aaaagaatct ttgctagagc
     2101 agataaaaaa acaccttgaa aacgatgagt cattgagtga tggtctaaaa atatccggct
     2161 atcatggata tggagttcac tatggtcaag agctttttcc ctactcacat tatcgttcaa
     2221 cttcaattcc tgctgatccg gagcatacag taaaaagaag ctctcagaaa aagactttta
     2281 ttattaataa agaactggat tagtataaaa tttttaacct atagttaatt gtttgcattt
     2341 aagatgaagt tattttatct ttagtagaaa agtgcttccc tatgagggaa aatcacaaat
     2401 catcataata cagagacaaa acgatacgaa aaaatagata aaaaacatca ttcttttacc
     2461 acattatttt ccgtgaatat gaaaaataat gttattactt taatataaga ctattttttg
     2521 tttttccatc actctgttca aatttttccg catgacttgt gttttttgta atacagctcg
     2581 ttttttacag ctgaccaaaa tcatcaatta attatgctaa ggaaataaat tatgaaaacc
     2641 aaaaactttc ttcttttttg tattgctaca aatatgattt ttatcccctc agcaaatgct
     2701 ctgaaggcag aaggttttct cactcaacaa acttcaccag acagtttgtc aatacttccg
     2761 ccgcctccgg cagagaattc agtagtattt caggctgaca aagctcatta tgaattcggc
     2821 cgctcgctcc gggatgctaa tcgtgtacgt ctcgctagcg aagatgcata ctacgagaat
     2881 tttggtcttg cattttcaga tgcttatggc atggatattt caagggaaaa taccccaatc
     2941 ttatatcagt tgttaacaca agtactacag gatagccatg attacgccgt gcgtaacgcc
     3001 aaagaatatt ataaaagagt tcgtccattc gttatttata aagacgcaac ctgtacacct
     3061 gataaagatg agaaaatggc tatcactggc tcttatccct ctggtcatgc atcctttggt
     3121 tgggcagtag cactgatact tgcggagatt aatcctcaac gtaaagcgga aatacttcga
     3181 cgtggatatg agtttggaga aagtcgggtc atctgcggtg cgcattggca aagcgatgta
     3241 gaggctgggc gtttaatggg agcatcggtt gttgcagtac ttcataatac acctgaattt
     3301 accaaaagcc ttagcgaagc caaaaaagag tttgaagaat taaatactcc taccaatgaa
     3361 ctgaccccat aaagctggac agcctgtatc aggctatgga gggcccatag acaaatctac
     3421 cctatatgag cataggagga gtctatgggc acaccacgtt ttacccctga atttaaggga
     3481 ttactggaaa ggctgggaca tatccctccg gcagaagcag aaaaagctta ttatgctgcc
     3541 atcggaaacg atgatctggc aacctgagtt cacagataaa acattctcta ggaaactcgg
     3601 ggcggttccg ttcaccacat gcaatgtggt gttgcagggg aacggtctgc accatcccct
     3661 atgtcgatca atataacaga aatgacaact tcagattcag ggcacaacct aaatatattt
     3721 taggtcacct ctcaaatcgt ttgcctgata ccgctccatt ttttaat
//
LOCUS       DAJTSI010000243         3738 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000243 DAJTSI010000000
VERSION     DAJTSI010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.248"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            108..2105
                     /gene="betT"
                     /locus_tag="OO327_003957"
     CDS             108..2105
                     /gene="betT"
                     /locus_tag="OO327_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HCR6020297.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2168..3445
                     /locus_tag="OO327_003958"
     CDS             2168..3445
                     /locus_tag="OO327_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HCR6020298.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATRADHIDSDRYLIKSTYYNLFSGEYSNKKT"
BASE COUNT          905 a          852 c          940 g         1041 t
ORIGIN      
        1 ttccaccatg cattacgatt aacgccgcat agccagttga actttgctac tttgtgagag
       61 gtagtacctt ctatccagtg cgaatttaat taatggaata aatgattatg agtgaaaatg
      121 atacaatccc aaagaagtct acaagtcaga ttaacaaagc ggtattcttt acatctgctt
      181 tgctaatttt ccttcttgtc gcctttgccg ccgtattccc ggatgtcgcc gacaaaaatt
      241 ttaaactact tcagcaacaa atcttcacga atgccagctg gttctacatc cttgctgtgg
      301 ccctgatttt actgagtgtc acgttccttg gactctcacg ctacggtgat atcaagctgg
      361 gcccggacca tgcgcagcct gatttcagct accactcctg gtttgcgatg cttttttcgg
      421 cagggatggg gatcggcctg atgttctttg gcgttgccga acctgtaatg cattatcttt
      481 cgccacccgt tggcactcca gaaaccgttg cggcagctaa ggaagcaatg cgtctgacct
      541 ttttccactg gggactgcac gcatgggcaa tttatgccat tgtggcgctg attctggcgt
      601 tcttcagtta ccgtcacggt ctgcctttaa ctctgcgctc cgcactctat cccattattg
      661 gcgatcgcat atacggacct gtaggacatg cggttgatat tttcgctgtt ataggcacgg
      721 tctttggcgt tgcgacatca ctgggttacg gtgttttgca ggtgaatgcc ggtttgaacc
      781 atcttttcgg ggtgcccatc aatgaaacgg tgcaggtaat tctgatcgtg gtcatcacgg
      841 ggttagcgac gatttcagtg gtgtccggtc tggataaggg aatacgtatc ctgtctgaac
      901 tcaatctggg tctggctttg ttgctcctgg cgctggtcct gtgtctggga ccaaccgtgc
      961 ttctgctgaa gtcatttgtg gaaaatacgg gcggttatct ttcggaactg gtgagtaaaa
     1021 cgttcaacct ttacgcgtat gagcccaagt cgagcaactg gctggggggc tggacattac
     1081 tgtactgggg atggtggctt tcatggtcgc cgtttgtggg gatgttcatc gcacgggtct
     1141 cccgcgggcg aaccattcgc gagtttgtca ccggcgtgct gtttgttccc gcgggtttta
     1201 cgctaatgtg gatgacggtg tttggtaaca gcgcgatcta tctcattatg aaccaggggg
     1261 ccacagacct cgccaatact gttcagcagg atgtggcgct ggccctgttt aatttcctgg
     1321 agcatttccc gttctcttct gtgctgtcat tcattgcaat ggcgatggtc atcgtcttct
     1381 ttgtaacgtc tgctgattcg ggggcaatgg ttgtggatac tctggcatca ggtggagtgg
     1441 caaacacacc cgtctggcag cgaatattct gggcctcgct catgggcatt gttgcaattg
     1501 cgcttctcct tgccggaggg ctaagtgcgc tgcaaacggt gacaatagcg agtgcattgc
     1561 ccttctcagt gatcttacta atatctatat acggactttt aaaagctttg cgccgggatt
     1621 tgaccaagcg tgaaagcctg agcatggcga caattgctcc tacggctgca cgtaacccaa
     1681 ttccttggca gagaaggtta cgcaatatcg cgtatctgcc gaagcgatct cttgtgaaac
     1741 gttttatgga cgacgttatc cagcctgcca tgacgctggt tcaggaggaa ctgaacaagc
     1801 aggggacgat aagccacatt agtgatgcag tcgacgatcg tattcgtctt gaagtcgatt
     1861 tgggcaacga gctgaatttt atatatgaag tgaggcttcg cgggtatatc tcaccgacct
     1921 tcgcgctcgc cgcaatggat aatgatgagc agcagactga acaacatcga tattatcgcg
     1981 ctgaggttta tctcaaagaa ggcggtcaaa attatgatgt gatgggctgg aaccaggaac
     2041 agctgattaa tgacatactg gaccagtacg aaaaacacct gcacttcctg cacctggttc
     2101 gttaatagca acatgccgtc ctgggggcag caattattat cccggccgca atatgaggga
     2161 atgcagaatg atttcacgct ggaaatggat gctgaagcag acaattaaaa aactatggtt
     2221 cagggcaacg ttattcgcaa ttgtcgcgat aataacggcc cttttatcaa ttctttttaa
     2281 atcaatgata cctgagtcgg tttccgtgaa ggttggtgcg gaagcagtcg ataacattct
     2341 gaacatactg gcatcgagta tgctggcagt gaccacattt tcgctgagta tcatggtcac
     2401 agcctacggt tcagccacta ctaatgtgac tcccagagct acgcgtttag ttgttgaaga
     2461 cgtcaccaca caaaatgtac tggccacctt catcggttct tttctcttca gtctggtagg
     2521 gattattgcc ctcaatatgg gagcttatgg agaaaggggg agagtcattt tattcattgt
     2581 cacactggtt gtcattgcct taatcctcat cacattgctt cgctggatac agcatttgac
     2641 ctctctgggg agggttggtg agacaacggc acaagtagaa caggcggcca tcgaaacatt
     2701 tattgcgaga gcaagaaatc cctgtctcgg tggatatcca tggcttgaga acaatgaaca
     2761 gccgaaagga acggttgcag tttatccgaa gaagattggc tatgttgaat atattgatat
     2821 ggtgaaactt agcaagctgc tgaccaatga tccccgtcat gtatacctcg tggcgcagcc
     2881 aggcagtttc atacatccgt ccatgccagt tttgtacctg agtcagggcc aggagtcgtc
     2941 aatcaacacc gatttacttg agacgattat tgtctcggat gcacgttcat ttgctcagga
     3001 tcctcgattt tgtcttagcg tcatggccga aatagcctgc cgggcccttt cccctgcagt
     3061 gaacgatcct ggaaccgcca ttgatgtcat tggcagaggt gttcgtatac tttccactta
     3121 cgcgcagaat aaatctgatg aaatagaagt gaaatatcct tcagtacatg ttgcaccact
     3181 tcagaataac gatctactgg aagacttttt ctcacctgtc gcgcgcgatg gtgccagtat
     3241 gagggagatt cagataagag tccttaaagg actgtcgatg ctgagtaaag gttggcctga
     3301 gatatttgct gaagccgcac ataccctggc atttgaaaca ttagagcatg caactcgtgc
     3361 tgaccatata gattctgata gatatctaat aaaatcaact tattataatt tatttagtgg
     3421 cgaatattct aataaaaaaa catagctgca gagcaagcaa ttcggttgtt ggcaagtcaa
     3481 gggcaaagca ttcgcgtgag aatgggcgta tgctttgtgc caaaagcaaa cgttgctaat
     3541 tctgcactgt tcagcgatgg cgcagtagag acaggcactg ccactgtcca gtcggcaaaa
     3601 gcgaacactt tgccactgcg gggataaact gattgaagct ctcgcgcaaa atcacttatt
     3661 cttttgcgtt aataaaatgt aggagatggg tcattgatcc tacccacgta atatggacac
     3721 aggcctaagc gaggttct
//
LOCUS       DAJTSI010000244         3737 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000244 DAJTSI010000000
VERSION     DAJTSI010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.249"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            368..2020
                     /locus_tag="OO327_003959"
     CDS             368..2020
                     /locus_tag="OO327_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR6020299.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2031..2201)
                     /locus_tag="OO327_003960"
                     /pseudo
     CDS             complement(2031..2201)
                     /locus_tag="OO327_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2334..2453)
                     /locus_tag="OO327_003961"
                     /pseudo
     CDS             complement(2334..2453)
                     /locus_tag="OO327_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2527..3457)
                     /locus_tag="OO327_003962"
                     /pseudo
     CDS             complement(2527..3457)
                     /locus_tag="OO327_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766279.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1032 a          861 c          857 g          987 t
ORIGIN      
        1 atcggaaacg atgatctggc agcctgagtt cacagataaa acattctcca ggaaacttgg
       61 ggcggttcac gtcagtatca gaccatatta attgctgaaa ctgataaaca tcaaacctta
      121 tagaagggtg aatgctgaaa gggggctatg gtgatcgacg tgatgacaac gacaggtaag
      181 accgtgacct gataaacctg aattgtgtcc tagacataaa gtccgtgaag ggaataaggc
      241 tcaggtcagc gaatcttata gtggcccgca gctctgactg caataaggcc ggatacagag
      301 cagcaactac tcgtcgatgt cagaacaacg gaagccttgc aaacggggat gcgttcatac
      361 aggaacaatg ataccgttaa aaaaaaacat tacgctaata atgttcaccc tgtcattact
      421 gacaggaaat cctgctatag cgtatgaaac agataaagta tataaaataa ccgttctgca
      481 tacaaatgac caccatggac acttttggcg taacaatcat ggtgagtacg ggttatcctc
      541 ccaaaaaact ctggtggaca atatccggca gaaagtaata aataatggcg gaagtgttct
      601 attattatct ggtggtgata ttaacaccgg tgtacctgaa tcagaccttc agaaggctga
      661 acccgatatt cgtggaatga atctaattgg ttatgatgca atggccgtcg gtaatcatga
      721 atttgataat cctctaaata tattacgtca gcaggaaaaa tgggccacat ttcctttcct
      781 ttctgctaac atatatcaaa aaagtacagg gaggcgttta ttcagtccat ggaaaatttt
      841 catccgtcag aatttgaaaa ttgcagtcat tggcttgacg acagatgata ctgcgaaaac
      901 aggaaattct gaatatttta ctgatattga gttccgtcaa cctgctgctg aggcgaggag
      961 tgtcattgat gaacttaatc agcaggaaaa gccagatatt attattgcgg caacacatat
     1021 gggtcactat gacaatggcg agtcaggttc gaatgctcct ggtgatgttg aaatggctcg
     1081 cagtttgccg actggctcat tagcaatgat tgtgggagga cattcacaag ctcctgtctg
     1141 catggccagc gataataaaa aacagtggaa ttatattccg gggacaacct gtgtaccaga
     1201 taaacaaaat ggcatctgga ttgtacaagc gcatgagtgg ggaaaatatg tcggtcaggc
     1261 tgattttgaa ttctgtaacg gtacaatgaa actggttaat tatcagttac acccagttaa
     1321 tctcaaaatg agaattacca gagaagatgg aaaaacggaa ttttcttttt acacccccga
     1381 aattactgaa gatccacaga tgttatcctt actgacacca ttccagaata aaggcaaagc
     1441 acaactggat gtgaaggttg gggttgtgaa cggacgtctg gaaggggaca gaagtaaagt
     1501 tcgttttgtt cagaccagta tgggccatct gattctgagc gctctgacag aacgtattga
     1561 cgcagatttt gctgttgtca gtggtgggga aattcgagat tccatagaga gtggtaacat
     1621 cacatacaaa gatatactga aagttcagcc atttggtaat acggtagtaa gtattgattt
     1681 gacggggaaa gaagtagctg actacctagc tacagtggca cagatgaaac cggattccgg
     1741 agcctaccca caatttttga ataccagctt tgtagtcaaa aaaggaaaaa tcgaaatgct
     1801 aaaaataaaa ggtaaatcgg ttgaccttaa caaaaaatat cgtatgacaa catttagctt
     1861 taatgctact ggtggggatg gctatccacg tattgataat agacccggat acataaatac
     1921 cggatttatt gatgctgaag tacttatcga atatatccgg aaacattcac cgctggatgc
     1981 tgcttcatat gaaccaaaag gtgaagtcag ttggcagtga taacgtaatt gacgatcatc
     2041 acgtgttttt tgctgacagt cgctgtgact ttgtgaaagt agtctctgcg tatgtttgcg
     2101 atatgggaat gcagttgctg tattttgtgt ttctgcttct gccagttgtt gctgaattta
     2161 accttgcggc ttaactgatg ttggagcgct gccagcttct tcgtcaattt cgctgattaa
     2221 agtttattca ttatggcatg atctgcgtaa gcggctcgtc agaaccatat tgatatttac
     2281 taagagctca gatcaacttt ccagggcaac agatcgcgta cccggtttgc cggtcagtcc
     2341 tggatatgct caatgacgta acgcagccat ttttctggct ccacattgtt cagacggcat
     2401 gtgccgatca gcgagtacaa caccgccgca tgttcgccac cgctatcgga accccgccgg
     2461 agagagtttc actctccggc tgctcacgga accgtacgtg acactctcgc gtcatacggc
     2521 tcttgatcac tcattcgatg cacgtagtaa ataccagtgg gcaaatagct tcggatactg
     2581 tttccttacc agcgccagct ttcgctgagc cttccggttc gaaagtctgt atttagactg
     2641 catccactta agcaaacgtg actgcattgc actccacagt cgatagctga agtttctgga
     2701 ccagaacttt ccatagtact caatccagcc tctcactatc gcattgtagc gcctcgcaaa
     2761 atccagcaaa ctctcagctg ttgagcgatg tatacgccac ttcttgattg tttctgttat
     2821 tttgcacatt gctgcatttg atgcacccgg catacatttt ctgtacaatt cgcctttgaa
     2881 attcttcaga gtacgcactt tgaagtcata tccgagagtg gcatacaaca tcatcggcgt
     2941 acctttcgaa gggaacgccg ggaaactgac gttgcatcca catatcaaag gcatagtgca
     3001 ggaacagatt tgagagcagg ggggagatta cgccgccctg cggcgttcct cgaccccgtg
     3061 ccagcattgc tccatccggc atttgcaccg gtgccttcag cctgcgctca atgtacatca
     3121 ccacccagcg tgcctgagta tgatgctgca ctgtcttcag tagcaggtca tgatcgatat
     3181 catcaacgaa gcctttaata tcgacttcca ccacccagtc aaacttccag cagcgtttcc
     3241 tcgcctgcgc aatcgcctgg tgtgcggatt tacccggtcg ataaccatac gagtctgcat
     3301 ggaacagggg ttccagtacc ggctcgagta tctgcttaac gaccatctgg gcgatccggt
     3361 ccgacacggc cggaataccg agcggacgta caccaccgtc agctttggga atgtccacac
     3421 gccttaccgc ctgcggcata taactgccgg aagccattct gttccacagt ttgtagagat
     3481 tgtctgtaac attctcgtca aacccggcca gtgtctgccc gtcaatgcct gccgccccac
     3541 ggttggcttt tactcgttta taagcttccc atacctgtcg tttacttaca ggaaatggct
     3601 ttgccgattt tacagcagtc ctcccatcgg cattgtcgcc cgtggttgcc gccgcaccat
     3661 cagcacaatg gggcaagccc tttgctccgg acccattacg gtccttcacc gctactacgg
     3721 tttactccgt ccctgta
//
LOCUS       DAJTSI010000245         3724 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000245 DAJTSI010000000
VERSION     DAJTSI010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.250"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..503)
                     /gene="rutD"
                     /locus_tag="OO327_003963"
     CDS             complement(<1..503)
                     /gene="rutD"
                     /locus_tag="OO327_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCR6020300.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALA"
     gene            complement(511..897)
                     /gene="rutC"
                     /locus_tag="OO327_003964"
     CDS             complement(511..897)
                     /gene="rutC"
                     /locus_tag="OO327_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR6020301.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            complement(909..1601)
                     /gene="rutB"
                     /locus_tag="OO327_003965"
     CDS             complement(909..1601)
                     /gene="rutB"
                     /locus_tag="OO327_003965"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCR6020302.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(1601..2692)
                     /gene="rutA"
                     /locus_tag="OO327_003966"
     CDS             complement(1601..2692)
                     /gene="rutA"
                     /locus_tag="OO327_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCR6020303.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2980..3618
                     /gene="rutR"
                     /locus_tag="OO327_003967"
     CDS             2980..3618
                     /gene="rutR"
                     /locus_tag="OO327_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR6020304.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
BASE COUNT          867 a          991 c         1026 g          840 t
ORIGIN      
        1 gccagcgcgt cttctgcctc caggcgaggt gcgcgggccg ccatccagtc ggcgggatag
       61 aggaacaacg gctgcgcttc cacccatgct tgcgcgccgc cgctatacag taatcgttcg
      121 cgaacctgaa aacagcggcg cgtatgggcg tttattcgta gccagccgtt aacgctgacc
      181 agcatagtta ccgacgcggg ataatccagc gccagctgca tccccaccag cgcaccgagc
      241 gcatggccga ccaccgcgta acgctcaatc cctgcggcta ccagcgcctg atgcagttcc
      301 gctgccatct gggcgatact gtaatcttct gccagtgtgt cgggattatt gccggtgccg
      361 cgctggtcgt aacagactac ctgatactcc tgctccagca ccgccagttg cggtaaccag
      421 taactgccgc taccgccaag acccgaaatc aacaccacta cgggtgcatc agcataaggg
      481 ggagatgaga gtgaaagttt catcgcggcc tcacttggcg atatgcgcaa ttgtggcgat
      541 ttccaccagc gcgtcaggtt ttaccagtcc gcactgaatg tagaatcgcg ccggtttatc
      601 acccggaaaa aactcggcgt agatttcgtt aatcgcggcg taatttttcc agtcggtaat
      661 aaagatgctg ttgaaggtca catccgccat cgtgccaccc gccgtctcga tcaccttgcg
      721 gatagtttcc agaacgtggc gggtttgcgc ctttgggtca tcggcaaaca gcacgttatt
      781 atgttgatca aaagccagcg taccggagac atacaccacg ccatcagcca gcgtgccggg
      841 aacgaagggg gccagcggtg cgctgctgcc agcgggaata attacggatt ttggcatcgc
      901 taaactcctt aagcgatacg agcaaaggac gtgggagaaa gcgcgtcgca gaacgtttcg
      961 acgtcgctga cccagccaaa aaaggtttcg atattgaaca acgcggcttt ctgtgcaaat
     1021 tccggccccg cctggtgtgt tgcgtcttcc agcaccacgc cgaaatgctc cagaaaaaag
     1081 ccatcgcgta gcgtcgattc gacgcagacg ttggtagcga taccggtgaa aaccagatgg
     1141 cgtattccac ggctgcgtaa aatgctgtcc agcggcgtat tgaagaaacc gctgtagcgc
     1201 ggcttcggca gcacaatatc gccaggctgc ggcaccagtt catccaccag ttgataatcc
     1261 caggagcctt tcgccagcaa tttcccctgc agctgcggct gcttacgcat ggttttcagg
     1321 gcgttcgatt tatgaaaatt cggtgagccg gggccgccag cctcgacata ctgttcatcc
     1381 cagccatttt gaaaccagat gatcaacatc cctgccgctc gcgctgcggt cacagcggtt
     1441 tgaatgttgg caatgaccgg gcgagtggtt gagacatcaa acccggcgag atctaagtag
     1501 ccgcctggcg tggcataagc gttttgcata tccaccacga tctgcgcact ttgctgcgga
     1561 tcgaaggtaa tggcttccgg tcgagcggtt aaggttgtca tcatgccacc tcctgagtca
     1621 gcgcagagag atgggcgcgg cactgcatca gtggttgaat gcgctcgccg aaggtttcga
     1681 ttcccgacag aaaatcatcg aaggtcaaca gcacgccttc tgcaccaggc acgcttgcga
     1741 cttcatctaa catgcgcgcg acactggcgt aagaaccgac taacgtcccc atattgatgt
     1801 ttaccgccga agtgggatcg gccatctgac gaacgttggt gtcagtaccg gagcgggtat
     1861 ctttctgact ttgttcggtt agccagctta acgcctcttc atccgcgccc gctttgtagt
     1921 gttcccattt ggcgcgagtg gcatcgtcgg tttcatcggc aatcaccata aacaacacat
     1981 aagagccaac gtcgcgtccg gtttgctctg tggcctgttt catccgcgca gcggtcgggg
     2041 cgaaagccgt gggtgtattt acgcctttgc cgaaacagaa gttgaaatcg gcatactggg
     2101 cggagaacgc catgccagcg tcgctttgcc cggcgcagat cactttcatg gggatactcg
     2161 gttgcggact gacgcgacaa tcattcatgg tgaaaaaatc gcctttaaaa tcgcttttcc
     2221 ccgtgcccca caggtcgcgc agcacctgaa catattcggt gagatagtcg taacgacggg
     2281 agaaatagtc atcgccaggc cagataccca tctgctcata ctcgggcttt tgccagccag
     2341 tcacgaggtt gacgccaaaa cgcccgccag agatggagtc gatggttgcg gccatacggg
     2401 cgacgattgc cggaggtaac gttaaggtgg cagcagtggc gtaaatctga atgcgcgagg
     2461 tcacggctgc cagacccgcc atcaaggtga acgactcaag gttgtgatcc cagaactcag
     2521 ttttgccgcc aaagccacgc agtttgatca tcgacagggc gaaatcgaaa tggtaatgct
     2581 ccgctttttg cgcaatggct ttattcagtt caaaggtcgg catgtactgc ggcgcgtggg
     2641 tcgaaatgag ccagccgttg ttgccaatag gtacgaatac gccaattttc atcatcaacc
     2701 tctcttcgtc tcgtaaagtg aaagtcagac ggggcgctgc atcctgcata tccttttcag
     2761 ccgcgtattg gcttgttcgc aaagcggatg ccagttttta aaaagtgaat attattaatg
     2821 tgttagcatt gtgttatcta aaatatctgg taaaaagtgg actaaacggt caaaacagtt
     2881 gcacataaaa catgcatccg tgcgcgatga gagtgcagaa ggtcgaggcc gggcgggggt
     2941 tttgctatcc tgttaccaat ctacaagagg ggagagcgca tgacgcaagg cgcagtgaaa
     3001 acaacgggta aacgttcgcg cgcagtaagc gcgaagaaaa aagcgattct tagcgcagca
     3061 ctggacactt tttcacaatt cggttttcac ggcacaaggc tggagcagat cgcagagttg
     3121 gcgggtgttt caaaaaccaa tctgctgtat tacttcccgt caaaagaggc gctgtatatt
     3181 gccgtgctgc ggcagattct cgatatctgg ctggcaccgt taaaagcgtt tcgtgaagat
     3241 ttcgccccgc tggcggcgat aaaagagtac atccgtctga agctggaagt ctcacgcgat
     3301 tatccgcagg cttcgcgcct gttctgtatg gagatgctgg caggcgcgcc gttgttaatg
     3361 gatgaactga cgggcgattt gaaggcatta attgatgaga aatcggcgct gattgccggt
     3421 tgggtcaaaa gcggcaaact cgcgccgatt gatccgcagc atttgatttt tatgatttgg
     3481 gcttccactc aacattacgc cgatttcgcc cctcaggtgg aggcggtgac aggcgcgacg
     3541 ttgcgcgatg aggtattttt caatcaaacg gttgaaaacg tgcagcggat tattattgag
     3601 gggattcgac cacgttaaag atgacggagg aggttgtaac atcctccggc tacctgttta
     3661 accgatcgtc attaaactgg cgttaccgcc agcggcagcg gtattcacac tcagcgaacg
     3721 ttca
//
LOCUS       DAJTSI010000246         3586 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000246 DAJTSI010000000
VERSION     DAJTSI010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.251"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..641
                     /gene="tolA"
                     /locus_tag="OO327_003968"
     CDS             <1..641
                     /gene="tolA"
                     /locus_tag="OO327_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR6020305.1"
                     /translation="KAATEAAEKAKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKA
                     AADKAAADKKAAAEKAAADKKAAAAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTG
                     GGAKGNNASPAGSGNTKNNGASGADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKL
                     APDGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            774..2066
                     /gene="tolB"
                     /locus_tag="OO327_003969"
     CDS             774..2066
                     /gene="tolB"
                     /locus_tag="OO327_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR6020306.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            2101..2622
                     /gene="pal"
                     /locus_tag="OO327_003970"
     CDS             2101..2622
                     /gene="pal"
                     /locus_tag="OO327_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR6020307.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            2632..3423
                     /gene="cpoB"
                     /locus_tag="OO327_003971"
     CDS             2632..3423
                     /gene="cpoB"
                     /locus_tag="OO327_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR6020308.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
BASE COUNT          965 a          858 c          957 g          806 t
ORIGIN      
        1 agaaagcggc aactgaagct gctgaaaaag ccaaggcaga agctgagaag aaagcggctg
       61 ctgaaaaggc tgcagctgat aagaaagcgg cagcagagaa agctgcagcc gacaaaaaag
      121 cagcagaaaa agcggctgct gataaggcag cagctgataa gaaagcagcg gcagaaaaag
      181 ccgccgcaga caaaaaagcg gcagcggcaa aagcagcagc tgaaaaagcc gctgcagcaa
      241 aagctgccgc agaggcagat gatattttcg gtgagctaag ctctggtaag aatgcaccga
      301 aaacgggggg aggggcgaaa gggaacaatg cttcgcctgc cgggagtggt aatactaaaa
      361 acaatggcgc atcaggggcc gatatcaata actatgccgg gcagattaaa tctgctatcg
      421 aaagtaagtt ctatgacgca tcgtcctatg caggcaaaac ctgtacgctg cgcataaaac
      481 tggcacccga tggtatgtta ctggatatca aacctgaagg tggcgatccc gcactttgtc
      541 aggctgcgtt ggcagcagct aaacttgcga agatcccgaa accaccaagc caggcagtat
      601 atgaagtgtt caaaaacgcg ccattggact tcaaaccgta atcgcgatgt tgactgttcg
      661 gacggtcaac atcaggcacc ggttgccacg gggttctggt agttttgtgt attttagttt
      721 gttaacattc tgctaaatta tcgtgggcca tcggtccaga taagggagat atgatgaagc
      781 aggcattacg agtagcattt ggttttctca tactgtgggc atcagttctg catgctgaag
      841 tccgcattgt gatcgacagc ggtgtagatt ccggtcgtcc tattggtgtt gttcctttcc
      901 agtgggcggg gcctggtgcg gcacctgaag atattggcgg catcgttgct gctgacttgc
      961 gtaacagcgg taaatttaat ccgttagatc gcgctcgtct gccacagcag ccgggtagtg
     1021 cgcaggaagt acaaccagct gcatggtccg cactgggcat tgacgctgta gttgtcggtc
     1081 aagtcactcc gaatccggat ggttcttaca atgttgctta tcaacttgtt gacactggcg
     1141 gcgcaccggg tactgtactt gctcagaact cgtacaaagt gaacaagcag tggctgcgtt
     1201 atgctggtca taccgccagt gatgaagtgt ttgaaaaact gaccggcatt aaaggtgcgt
     1261 tccgtacccg tattgcctac gttgttcaga ccaacggcgg tcagttcccg tatgaactgc
     1321 gcgtatctga ctatgacggt tacaaccagt ttgtcgttca ccgttcaccg cagccgctga
     1381 tgtctccggc gtggtcacca gacggttcta aactggctta tgtgaccttc gaaagcggtc
     1441 gttccgcgct ggttattcag acgctggcaa atggcgctgt acgtcaggtg gcttcattcc
     1501 cgcgtcacaa cggtgcacct gcattctcgc cagacggcag caaactggca ttcgccttgt
     1561 cgaaaaccgg tagtctgaac ctgtacgtaa tggatttggc ttctggtcag atccgccagg
     1621 tgactgatgg tcgcagtaac aataccgaac cgacctggtt cccggatagc cagaacctgg
     1681 catttacttc tgaccaggcc ggtcgtccgc aggtttataa agtgaatatc aacggcggtg
     1741 cgccacaacg tattacctgg gaaggttcgc agaaccagga tgcggatgtc agcagcgacg
     1801 gtaaatttat ggtaatggtc agctccaatg gtgggcagca gcacattgcc aaacaagatc
     1861 tggcaacggg aggcgtacaa gttctgtcgt ccacgttcct ggatgaaacg ccaagtctgg
     1921 cacctaacgg cactatggta atctacagct cttctcaggg gatgggatcc gtgctgaatt
     1981 tggtttctac agatgggcgt ttcaaagcgc gtcttccggc aactgatgga caggtcaaat
     2041 tccctgcctg gtcgccgtat ctgtgataat aattaattga atagtaaagg aatcattgaa
     2101 atgcaactga acaaagtgct gaaagggctg atgattgctc tgcctgttat ggcaattgcg
     2161 gcatgttctt ccaacaagaa cgccagcaat gacggcagcg aaggcatgct gggtgccggc
     2221 actggtatgg atgcgaacgg cggcaacggc aacatgtctt ccgaagagca ggctcgtctg
     2281 caaatgcaac agctgcagca gaacaacatc gtttacttcg atctggacaa gtacgatatc
     2341 cgttctgact tcgctcaaat gctggatgca catgcaaact tcctgcgtag caacccgtct
     2401 tacaaagtca ccgtagaagg tcacgcggac gaacgtggta ctccggaata caacatctcc
     2461 ctgggtgaac gtcgtgcgaa cgccgttaag atgtacctgc agggtaaagg cgtttctgca
     2521 gaccagatct ccatcgtttc ttacggtaaa gaaaaacctg cagtactggg tcatgacgaa
     2581 gcggcatact ccaaaaaccg tcgtgcggta ctggtttact aagagaattg catgagcagt
     2641 aacttcagac atcaactatt gagtctgtcg ttactggttg gtatagcggc cccctgggcc
     2701 gcttttgctc aggcaccaat cagtagtgtc agctcaggct cggtcgaaga ccgcgtcact
     2761 caacttgagc gtatttctaa tgctcacagc cagcttttaa cccaactcca gcaacaactt
     2821 tctgataatc aatccgatat tgattccctg cgtggtcaga ttcaggaaaa tcagtatcaa
     2881 ctgaatcagg tcgtggagcg gcaaaagcag atcctgttgc agatcgacag cctcagcagc
     2941 ggtggtgcag cggcgcaatc aaccagcagc gatcaaagcg gtgcgacggc atcaacgacg
     3001 ccgacagctg atgctggtac tgcgaatgct ggcgcgccgg tgaaaagcgg cgatgcaaac
     3061 acggattaca atgcagctat tgcgctggtg caggataaat cccgccagga tgacgcaatg
     3121 gtggcatttc agaatttcat caaaaattac cctgattcaa cttacctgcc aaacgccaat
     3181 tattggctgg gtcagttaaa ctacaacaag ggtaaaaaag atgatgcggc gtactatttt
     3241 gcttcggtag tgaaaaacta tccgaagtca ccaaaggctg ctgatgcgat gtttaaagtc
     3301 ggcgtcatca tgcaggacaa aggtgacacc gcaaaagcga aagccgtata ccagcaggtt
     3361 atcagtaaat accctggtac tgatggcgct aaacaggcac aaaaacgtct gaacgcgatg
     3421 taatgcataa cacacgacca gaagtcgcat tatttctggt cgtgtcgtgc gaatcataag
     3481 cagttgagtg atctgcatcg aaatttttgt tgcgctcaag tctgaaatca gtaatatatg
     3541 ccgccgttgc cacgggatat caaacaaacc gaaagcaacg aaaaag
//
LOCUS       DAJTSI010000247         3549 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000247 DAJTSI010000000
VERSION     DAJTSI010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.252"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..465
                     /gene="pgrR"
                     /locus_tag="OO327_003972"
     CDS             <1..465
                     /gene="pgrR"
                     /locus_tag="OO327_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR6020309.1"
                     /translation="RLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLID
                     HQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYD
                     QVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            802..2415
                     /gene="mppA"
                     /locus_tag="OO327_003973"
     CDS             802..2415
                     /gene="mppA"
                     /locus_tag="OO327_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR6020310.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="OO327_003974"
     CDS             complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="OO327_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR6020311.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1006 a          773 c          820 g          950 t
ORIGIN      
        1 cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca
       61 atggctattg ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca
      121 caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc
      181 tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg
      241 aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct
      301 tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc
      361 actccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca
      421 ttctcattat ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata
      481 cattaattta attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt
      541 tattcttcgt acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc
      601 gaagcccgaa cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt
      661 tacactaaat ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt
      721 ttttgatgga agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta
      781 atgtcggtta gagggaaact tatgaagcac tctgtttcag ttacgtgttg tgcgctgttg
      841 gtcagcagca tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag
      901 aagcaggagc tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc
      961 gtgggcctgc cagagattca ggtcattcgc gatctgtttg aaggtctggt gaatcagaac
     1021 gaaaaagggg agattgtccc cggcgtcgcg actcaatgga aaagtaatga caaccgtatc
     1081 tggactttta ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa
     1141 gattttgtct acagctggca acgtctggtg gacccaaaaa cattgtcgcc atttgcatgg
     1201 tttgccgcgc tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct
     1261 gatcagcttg gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg
     1321 ttgccgtggt ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac
     1381 gtagaaagcg gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt
     1441 cttaaagagc gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat
     1501 aacgccaaaa cggtgctgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc
     1561 actaagcgtt acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat
     1621 cagaagctgt tgaaggatat tccggggcag gtttatacgc caccgcagct cgggacctat
     1681 tattatgcct ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta
     1741 agtatgacga tagatcgccg cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca
     1801 gcgtggcatt ttataccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa
     1861 caaatgagtc aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat
     1921 ggtccgcaaa aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa
     1981 attgcgattg ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa
     2041 aatcaggaat ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc
     2101 gcctcatggg tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaatg
     2161 cattcaggaa atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca
     2221 tcgacggaaa ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc
     2281 atggagcaag caccgattgc accaatttat caatatacca atggacgatt aatcaaaccg
     2341 tggctgaaag gttatcccat taataaccct gaagatgtag cgtacagtcg gactatgtat
     2401 attgtgaagc attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt
     2461 ctgatttagc gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg
     2521 aaggcaaagt ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc
     2581 tgtgcagcaa gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc
     2641 aatgacgagt cagcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc
     2701 gggtgatttt tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc
     2761 tcataacgta aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct
     2821 acgctgatcg acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta
     2881 attttggtaa ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa
     2941 cggatccagt cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa
     3001 ttactcagaa tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc
     3061 agcaagccag aaaggctcat gccgaaatgt tcgccataaa gcagaacaag cactacaata
     3121 atggtgattt tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta
     3181 atctgctgtt tttcaaactg attaatcagc agaaatagct taatcgtcag aataaccgca
     3241 atcagggacg tacagataaa atcgataacg cctggggtga taaatttgag tttatagttt
     3301 tctataacat aattactgat gctaccaaca gcactgataa ttatggtgta gattaaaaat
     3361 tgcaccgcat ggaataaaaa tccttttctt ttacgatttc cacggcgaaa ccaaaagctc
     3421 atcagaatca atgctgcgca gctaccgaaa ataatgacca gattaagcgc attatttgta
     3481 aacagttcag cgatcattgt tttatcaggc tcctccagat aattgtcgtc atgccggaaa
     3541 cccctggcg
//
LOCUS       DAJTSI010000248         3501 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000248 DAJTSI010000000
VERSION     DAJTSI010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.253"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..513
                     /locus_tag="OO327_003975"
     CDS             <1..513
                     /locus_tag="OO327_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HCR6020312.1"
                     /translation="KLARLIEIKASRDGRAADYAKEFGLVYLEGQQPWSVPVDIALPC
                     ATQNELDVDAAHQLIANGVKAVAEGAYMPTTIEATELFQQAGVLFAPGKAANAGGVAT
                     SGLEMAQNAARLGWKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKV
                     ADAMLAQGVI"
     gene            complement(630..1670)
                     /locus_tag="OO327_003976"
     CDS             complement(630..1670)
                     /locus_tag="OO327_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6020313.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(1797..>3501)
                     /gene="topB"
                     /locus_tag="OO327_003977"
     CDS             complement(1797..>3501)
                     /gene="topB"
                     /locus_tag="OO327_003977"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR6020314.1"
                     /translation="VIKRFLHEASEIVHAGDPDREGQLLVDEVLDYLQLAPEKRQQVQ
                     RCLINDLNPQAVERAIDRLRSNSEFVPLCVSALARARADWLYGINMTRAYTILGRNAG
                     YQGVLSVGRVQTPVLGLVVRRDEEIENFVAKDFFEVKAHIVTPADERFTAIWQPSEAC
                     EPYQDEEGRLLHRPLAEHVVNRISGQPAIVTSYNDKRESESAPLPFSLSALQIEAAKR
                     FGLSAQNVLDICQKLYETHKLITYPRSDCRYLPEEHFAGRHAVMNAISVHAPDLLPQP
                     VVDPDIRNRCWDDKKVDAHHAIIPTARSSAINLTENEAKVYNLIARQYLMQFCPDAVF
                     RKCVIELDIAKGKFVAKARFLAEAGWRTLLGSKERDEENDGTPLPVVAKGDELLCEKG
                     EVVERQTQPPRHFTDATLLSAMTGIARFVQDKDLKKVLRATDGLGTEATRAGIIELLF
                     KRGFLTKKGRYIHSTDAGKALFHSLPEMATRPDMTAHWESVLTQISEKQCRYQDFMQP
                     LVGTLYQLIDQAKRTPVRQFRGIVAPGSGGSADKKKAAPRKRSAKKSPPADEAGSGAI
                     A"
BASE COUNT          844 a          934 c          834 g          889 t
ORIGIN      
        1 aaactggcac gtcttatcga aatcaaagcc agccgcgatg gtcgagcggc agattacgcc
       61 aaagaatttg gtctggtcta tctcgaaggc caacagccgt ggtctgtacc ggttgatatc
      121 gccctgcctt gcgccaccca gaacgaactg gatgttgacg ccgcgcatca gcttatcgcc
      181 aatggcgtta aagccgtcgc tgaaggggca tatatgccga ccaccatcga agcgactgaa
      241 ctgttccagc aggcaggcgt actgtttgca ccgggtaaag cggctaatgc tggcggtgta
      301 gcaacgtcgg gcctggaaat ggcacaaaac gctgcgcgcc tgggctggaa agccgagaaa
      361 gttgacgcac gtttgcatca catcatgctg gatatccacc atgcctgtgt tgagcatggt
      421 ggtgaaggtg agcaaaccaa ctacgtgcag ggcgcgaaca ttgccggttt tgtgaaggtt
      481 gccgatgcga tgctggcgca gggtgtgatt taagttgtaa atgcctgatg gcgctacgct
      541 tatcaggcct acagatgggc acaattcatt gcagttacgc tctaatgtag gccggtcaag
      601 cgcagcgccc ccggcaaaat ttcaggcgtt tatgagtatt taacggatga tgctccccag
      661 ggaacatttc ttatgggcca acggcatttc ttactgtagt gctcccaaaa ctgcttgtcg
      721 taacgataac acgcttcaag ttcagcatcc gttaactttc tgcgatagca gcagatatgc
      781 cagtaaagaa atcccattag actatttttt tgataatctt cttcgctttc gaacaactcg
      841 tgcgcctttc gagaagcaag cattatataa tgccaggcca gttcttcttc aattgtcccg
      901 ttttgaaaag ctgtgcttga tatcgagatc atccatgata attccgccgc ccatattagc
      961 ttcgccgagg atttaccgga gcgattatta gcgcaatcag agatatagtc tgagggaaaa
     1021 acagcaaatt tattcaacaa ggcgataacc tgctctgggg cttcctccat gtttgcttta
     1081 aaggtattgg ctccatggtc gccagaaaga aaatgctcca ttaaggcaca ataactttcg
     1141 ctatcttcga taccccattg attctctaaa gactcgcgtc ttttactgat gatatcgatc
     1201 gagtcaaaag gaagcacatg atattggaag gtatctttgc caggttcagg ctttcgcggc
     1261 cagaactcta gcgtttcaga ccattgctta tgatagaatc gataaggtgc gatcaattgt
     1321 agcgcctgta acttctcgat actgagcggc tcaatacctt tagcctgata ataatgcagt
     1381 tgttcttttt ttgctttaaa accggcccga acaataagcc ccatcataat taatagataa
     1441 agaaaagagc atcccgcggt aatcaggcct ctttcattca aaccgttgga tgttatcgct
     1501 gcgaatacaa acattacagc gacaacacat gttaaataaa acccccactt acaaagcagc
     1561 atggccttat tttctttaat cacccgttca aaattactat taaatattcc ccagccatta
     1621 aaagaatact tctcgctccc aggatggttt tgcaataaaa cttttttcat cacattccct
     1681 gttattacat actttagagg cgataatttt atccatgcaa aaaaatatcc aacgaacttt
     1741 cttgatccgc tggatatttc agaatatgac tcgaatagca cgaaagattc gctcgcttac
     1801 gctatcgccc cgcttccggc ttcatctgct ggcggacttt ttttcgcact acgtttacgc
     1861 ggtgcagcct ttttcttatc agcactgcca ccactgcccg gagccacaat gccgcgaaac
     1921 tgccgcaccg gcgtacgttt ggcttgatca ataagctgat atagcgtccc caccagcggc
     1981 tgcataaagt cctgatagcg acactgcttt tcgctgattt gcgtcagcac cgattcccag
     2041 tgcgcggtca tgtccggtcg cgtcgccatt tctggcagcg aatggaataa cgcttttccg
     2101 gcgtcggtgg agtggatata gcgccctttt ttggtcagaa aaccacgctt gaacagcagt
     2161 tcaataatcc cggcacgggt tgcttctgtc cccagaccat cggtcgcacg aaggaccttt
     2221 ttcagatctt tgtcctgcac aaagcgcgcg atcccggtca tcgccgaaag cagtgttgca
     2281 tcggtaaaat ggcgcggcgg ctgggtttgc cgctctacca cttcaccttt ttcacacagc
     2341 aactcatcgc ctttcgccac cacaggcaat ggcgtgccgt cgttttcttc atcgcgctct
     2401 ttgctgccta acagcgtgcg ccagcctgct tcagcaagaa aacgggcttt agcgacaaat
     2461 ttgcctttgg caatgtccag ttcgataaca cacttgcgga acaccgcatc cgggcagaac
     2521 tgcatcaggt attgacgggc aatcaggtta tagaccttcg cttcgttctc cgtcaggttg
     2581 atcgcagaac tccgtgcggt cgggatgatg gcgtggtgcg catcgacctt tttgtcatcc
     2641 caacagcggt tgcgtatatc tggatctacc actggctgcg gcaacagatc cggtgcatga
     2701 acgctgatgg cattcatcac cgcgtggcgt ccggcaaaat gttcttctgg caaatagcga
     2761 caatcggaac gcggataagt gattagcttg tgcgtttcgt acagcttctg gcagatatca
     2821 agcacgttct gcgcgctcag accgaagcgt tttgccgctt caatctgcaa cgccgaaagc
     2881 gaaaacggca gcggcgcgga ttctgattcc cgtttatcgt tatagctggt gacaatagcc
     2941 ggttgaccac taatgcggtt aaccacatgc tccgccagtg gacgatgtaa caagcgccct
     3001 tcttcatcct ggtacggttc acacgcttcg ctcggttgcc agatagcggt aaaccgctca
     3061 tcggcaggtg tcacgatatg tgctttgact tcaaagaagt ctttcgccac gaagttttca
     3121 atctcttcat cgcggcgcac caccagcccg agcacgggtg tctgcacgcg tcccacggaa
     3181 agtacgccct gataaccggc attgcgaccg agaatggtat acgcacgggt catattgatg
     3241 ccgtacagcc agtcggcacg cgctcgcgcc agcgcagaaa cgcacaatgg cacaaactca
     3301 ctgttggaac gaagacggtc gatcgcccgc tcaaccgcct gcgggttcag gtcgtttatc
     3361 aagcaacgct gcacctgctg gcgcttttcc ggtgccagtt gcagatagtc cagcacttca
     3421 tccaccagca attgcccttc acgatccggg tccccggcgt gaacgatttc gctggcttca
     3481 tgcaggaagc gtttgatgac g
//
LOCUS       DAJTSI010000249         3493 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000249 DAJTSI010000000
VERSION     DAJTSI010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.254"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..311
                     /locus_tag="OO327_003978"
     CDS             <1..311
                     /locus_tag="OO327_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR6020315.1"
                     /translation="LDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQ
                     YYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA
                     "
     gene            468..1217
                     /locus_tag="OO327_003979"
     CDS             468..1217
                     /locus_tag="OO327_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR6020316.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1239..1805
                     /gene="yieF"
                     /locus_tag="OO327_003980"
                     /gene_synonym="chrR"
     CDS             1239..1805
                     /gene="yieF"
                     /locus_tag="OO327_003980"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR6020317.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="OO327_003981"
     CDS             complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="OO327_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR6020318.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPDYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3361..>3493
                     /locus_tag="OO327_003982"
     CDS             3361..>3493
                     /locus_tag="OO327_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="YieH; catalyzes the dephosphorylation of
                     phosphoenolpyruvate, AMP and p-nitrophenyl phosphate;
                     purine and pyrimidine nucleotides are secondary
                     substrates; member of the haloacid dehalogenase-like
                     hydrolases superfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCR6020319.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     "
BASE COUNT          961 a          908 c          863 g          761 t
ORIGIN      
        1 cactggacaa tgtgttacag gagctggggc gcgaacgcac gattgctatg agcctgccgg
       61 aattcgagca gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc
      121 cgcgctactg tcagtactac aatcaactcc atcaactgcc gttggttgct cttcctctcc
      181 cgtttgacga aagccagcaa aaaaagctgg aagttccttt taccctgctg tggcataaac
      241 ggaacagcca taatccgaag atcgtctggt tacgggaaac cattaaaaac ctttacgcgt
      301 cgatggcata accgaatcgt atgaaacgcg acccaatttc acaataaaat gtaaaaaagt
      361 tgtaataagc ttgtctgaat cgaactttta gccgctttag tctgtccatc attccagtaa
      421 atgattactc ttgtattcat aatggaccat taagcatgga gcgaaaaatg gcgactcact
      481 ttgcccgagg gattttaacg gaaggacatc tgatttctgt tcgtctcccc tcccagtgtc
      541 atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg ttttctggcg tccagaggtt
      601 tactcgcaga actgatgttc atgctgtatg gcattggcga attgccggaa atcgtcaccc
      661 tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc ttcgttttct atttcctatg
      721 ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga atgtggcctc gatatggaac
      781 tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc cgataaccac accttttcca
      841 gcaatgaatc gctatggatc agtaaacaaa acgatcctaa cgaagcgcgg gcgcagctca
      901 ttacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt tttgaatgac gatccgcgcg
      961 atctgcagct gctgcccatt gccggacgcc tgaaatgtgc tcatgtaaat catgtagaag
     1021 cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc ggtcacgccc gcgattgaaa
     1081 agctcagtgt ctgggagtta gatggcaaac acagctggaa aagcctgccg gatattcaca
     1141 gccgcgccaa caatcctacc agccggatga tgcgttttgc ccagctctct accgtgaagt
     1201 ctttttcgcc aaattgatag acaaccacag gagtcattat gtctgaaaaa ttgcaggtgg
     1261 ttacgttact ggggagcctg cgcaaaggct catttaatgg catggttgca cgtaccctgc
     1321 cgaaaattgc tccggcgagc atggaagtca atgcgttacc ttccattgcc gacattcccc
     1381 tgtatgacgc tgacgtacag caggaagaag gttttccagc aacggttgaa gctctggcgg
     1441 aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc ggaatataac tactcggtac
     1501 cgggtgggct gaaaaatgcc atcgactggc tttcccgcct gccggatcaa ccgctggccg
     1561 gtaaaccggt gttgattcag accagctcaa tgggcgtgat tggcggcgcg cgctgtcagt
     1621 atcacctgcg ccagattctg gttttcctcg atgcaatggt gatgaacaag ccggaattta
     1681 tgggcggcgt gattcagaac aaagttgatc cgcaaaccgg agaagtgatt gatcagggta
     1741 cgctggacca cctgaccggg caattgaccg catttggtga gtttattcag cgagttaaga
     1801 tctaaataaa aaacccgcca gtaatcatgc atggcgggtt tttaatgcgc tatcgatttt
     1861 aatgagcgtc gataaataca atcttcagga taaacagcag cgcaacgatg attacgcacg
     1921 ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac gcagtaggag ataaagccca
     1981 gcgcaatacc ttcggtaatc gaaaagctga acggcatcat cacggcggta ataaacgccg
     2041 gaacagattc agtaagatcc tgccagttca cgcgagcaag acttgaggtc atcaacacgc
     2101 caacgtagat caacgcgcca gctgcagcgt agtctggcac cattcccgcc agcggcgaca
     2161 gaaagataac cagcaggaac agcagaccaa caaccactgc cgtcagaccg gtacgaccgc
     2221 caaccgatac gccggaagag gactcaatat aagccgtaac ggaagaagta ccgataaacg
     2281 aaccggtcac ggaagagata ctgtcgacat acagcgcctg cttcatgcgc gggaatttcc
     2341 ccttctcatc cgccagacct gctttatcgg tcacgccaat cagcgtaccg gaggagtcaa
     2401 acaagttgac caacatgaaa gagaaaatca cccctgccag cccgaggtta aacgacccgg
     2461 ctaaatctac atgacccaca actgtcatta cgctcggcgg cgcagaaacg atgccattgt
     2521 agtgcacatc acccagcatc cagcccagca gcgtcgtcac cacgatagaa accagcaccg
     2581 ctgcgtgaat gttgcgcgag gccagaatag caatgatgaa aaagccgagg atacccagaa
     2641 gtacgctgtg agaagtcaga ttaccgatgc tcaccagcgt ttccgagtta gcgacaatca
     2701 cacctgcgtt tttcagcccc atcatgccga taaacaggcc gataccgctg gtaataccca
     2761 cacgcagact taccggaatg ttagcaatca tccagtaacg aacgcggaaa atggtcaaaa
     2821 gaagcagtcc gacagcgccc cagaagattg cgcccatacc gatctgccac ggcagcccca
     2881 ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc catcgcagga gccagcgcca
     2941 ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa agcagcaatc agacaggttg
     3001 ttacgaagac ggcactggtg tccatgccag caacgcccag aatttgtggg ttaacaaata
     3061 cgatgtagac catcgtcagg aaggtggtga acccggcaat cacttcagtt cgtgccgtag
     3121 taccatgttc gcgcagttta aaaacacgtt ccagcatcct ctgaccagaa gtctgggtgg
     3181 tatgttgatg actcattatc tatttccgaa caaggaggga aaatccgtcg ctatcgtata
     3241 ccaaaatgcg acaataggcg cgtttgtgag agactttttt attggattta cttatacggc
     3301 aacgattgcg ttgcgcaaat cggctctacg aaaacgttaa actgattaaa aaggaaaggc
     3361 atgtcccgga tagaagcggt atttttcgac tgcgacggta cgctggtcga cagtgaagtc
     3421 atttgctccc gcgcatatgt aacgatgttt caggaatttg gtattacgct cgatcctgaa
     3481 gaggtattca aac
//
LOCUS       DAJTSI010000250         3488 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000250 DAJTSI010000000
VERSION     DAJTSI010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.255"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..389
                     /locus_tag="OO327_003983"
     CDS             <1..389
                     /locus_tag="OO327_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR6020320.1"
                     /translation="HGVHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITEN
                     GSMITLSGIQYFHEMGIDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGW
                     LTRHLGYREVTITEKGYAAFKTHFHI"
     gene            complement(367..990)
                     /gene="tetR(B)"
                     /locus_tag="OO327_003984"
     CDS             complement(367..990)
                     /gene="tetR(B)"
                     /locus_tag="OO327_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR6020321.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1072..2277
                     /gene="tet(B)"
                     /locus_tag="OO327_003985"
     CDS             1072..2277
                     /gene="tet(B)"
                     /locus_tag="OO327_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR6020322.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2390..2983)
                     /gene="tetC"
                     /locus_tag="OO327_003986"
     CDS             complement(2390..2983)
                     /gene="tetC"
                     /locus_tag="OO327_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR6020323.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            3116..3487
                     /locus_tag="OO327_003987"
     CDS             3116..3487
                     /locus_tag="OO327_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6020324.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
BASE COUNT          948 a          641 c          716 g         1183 t
ORIGIN      
        1 accatggcgt acatgccaaa gtttccacgc cagtgcattt acgaaaagca cgtacttgct
       61 atgatcattt agctggcgaa gttgccgtta agatctatga ttccctttgt caacagcaat
      121 ggatcactga aaatggttca atgatcacat taagtggtat tcaatatttt catgaaatgg
      181 gaattgacgt tccttccaaa cattcacgta aaatctgttg tgcgtgttta gattggagtg
      241 aacgccgttt ccatttaggt gggtacgttg gagccgcatt attttcgctt tatgaatcta
      301 aagggtggtt aactcgacat cttggttacc gtgaagttac catcacggaa aaaggttatg
      361 ctgcttttaa gacccacttt cacatttaag ttgtttttct aatccgcata tgatcaattc
      421 aaggccgaat aagaaggctg gctctgcacc ttggtgatca aataattcga tagcttgtcg
      481 taataatggc ggcatactat cagtagtagg tgtttccctt tcttctttag cgacttgatg
      541 ctcttgatct tccaatacgc aacctaaagt aaaatgcccc acagcgctga gtgcatataa
      601 tgcattctct agtgaaaaac cttgttggca taaaaaggct aattgatttt cgagagtttc
      661 atactgtttt tctgtaggcc gtgtacctaa atgtactttt gctccatcgc gatgacttag
      721 taaagcacat ctaaaacttt tagcgttatt acgtaaaaaa tcttgccagc tttccccttc
      781 taaagggcaa aagtgagtat ggtgcctatc taacatctca atggctaagg cgtcgagcaa
      841 agcccgctta ttttttacat gccaatacaa tgtaggctgc tctacaccta gcttctgggc
      901 gagtttacgg gttgttaaac cttcgattcc gacctcatta agcagctcta atgcgctgtt
      961 aatcacttta cttttatcta atctagacat cattaattcc taatttttgt tgacactcta
     1021 tcattgatag agttatttta ccactcccta tcagtgatag agaaaagtga aatgaatagt
     1081 tcgacaaaga tcgcattggt aattacgtta ctcgatgcca tggggattgg ccttatcatg
     1141 ccagtcttgc caacgttatt acgtgaattt attgcttcgg aagatatcgc taaccacttt
     1201 ggcgtattgc ttgcacttta tgcgttaatg caggttatct ttgctccttg gcttggaaaa
     1261 atgtctgacc gatttggtcg gcgcccagtg ctgttgttgt cattaatagg cgcatcgctg
     1321 gattacttat tgctggcttt ttcaagtgcg ctttggatgc tgtatttagg ccgtttgctt
     1381 tcagggatca caggagctac tggggctgtc gcggcatcgg tcattgccga taccacctca
     1441 gcttctcaac gcgtgaagtg gttcggttgg ttaggggcaa gttttgggct tggtttaata
     1501 gcggggccta ttattggtgg ttttgcagga gagatttcac cgcatagtcc cttttttatc
     1561 gctgcgttgc taaatattgt cgctttcctt gtggttatgt tttggttccg tgaaaccaaa
     1621 aatacacgtg ataatacaga taccgaagta ggggttgaga cgcaatcgaa ttcggtatac
     1681 atcactttat ttaaaacgat gcccattttg ttgattattt atttttcagc gcaattgata
     1741 ggccaaattc ccgcaacggt gtgggtgcta tttaccgaaa atcgttttgg atggaatagc
     1801 atgatggttg gcttttcatt agcgggtctt ggtcttttac actcagtatt ccaagccttt
     1861 gtggcaggaa gaatagccac taaatggggc gaaaaaacgg cagtactgct cggatttatt
     1921 gcagatagta gtgcatttgc ctttttagcg tttatatctg aaggttggtt agttttccct
     1981 gttttaattt tattggctgg tggtgggatc gctttacctg cattacaggg agtgatgtct
     2041 atccaaacaa agagtcatca gcaaggtgct ttacagggat tattggtgag ccttaccaat
     2101 gcaaccggtg ttattggccc attactgttt gctgttattt ataatcattc actaccaatt
     2161 tgggatggct ggatttggat tattggttta gcgttttact gtattattat cctgctatcg
     2221 atgaccttca tgttaacccc tcaagctcag gggagtaaac aggagacaag tgcttagtta
     2281 tttcgtcacc aaatgatgtt attccgcgaa atataatgac cctcttgata acccaagagg
     2341 gcatttttta cgataaagaa gatttagctt caaataaaac ctatctattt tatttatctt
     2401 tcaagctcaa taaaaagccg cggtaaatag caataaattg gcctttttta tcggcaagct
     2461 cttttaggtt tttcgcatgt attgcgatat gcataaacca gccattgagt aagtttttaa
     2521 gcacatcatc atcataagct ttaagttggt tctcttggat caatttgctg acaatggcgt
     2581 ttaccttacc agtaatgtat tcaaggctaa ttttttcaag ttcattccaa ccaatgatag
     2641 gcatcacttc ttggataggg ataaggtttt tattattatc aataatataa tcaagataat
     2701 gttcaaatat actttctaag gcagaccaac catttgttaa atcagttttt gttgtgatgt
     2761 aggcatcaat cataattaat tgctgcttat aacaggcact gagtaattgt tttttatttt
     2821 taaagtgatg ataaaaggca cctttggtca ccaacgcttt tcccgagatc tcatctattg
     2881 aaacagcttg atagcctttt tcaacaaaca atattcgtgc tgagttaacc agtgattgat
     2941 aggtactctt aaaattttct tgttgatgat ttttattttc catgatagat ttaaaataac
     3001 ataccgtcag tatgtttatg gtatcatgat gatgtggtcg tgacaatctt aagaacattt
     3061 aggttatttt atgtatattg aacagcattc tcgctatcaa aataaagcta ataacatcca
     3121 attaagatat gatgataagc agtttcatac aacggttatc aaagatgttc tattatggat
     3181 tgaacataat ttagatcagt ctttactgct tgatgatgtg gcgaataaag cgggttatac
     3241 caagtggtat tttcagcggc tgttcaaaaa agtaacaggg gtcacactgg ctagctatat
     3301 tcgtgctcgt cgtttgacga aagcggctgt tgagttgagg ttgacgaaaa aaactatcct
     3361 tgagatcgca ttaaaatatc aatttgattc ccaacaatct tttacacgtc gatttaagta
     3421 catttttaag gttacaccaa gttattatcg gcgtaataaa ttatgggaat tggaggcaat
     3481 gcactgag
//
LOCUS       DAJTSI010000251         3456 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000251 DAJTSI010000000
VERSION     DAJTSI010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.256"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(226..429)
                     /locus_tag="OO327_003988"
     CDS             complement(226..429)
                     /locus_tag="OO327_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020325.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(431..586)
                     /locus_tag="OO327_003989"
     CDS             complement(431..586)
                     /locus_tag="OO327_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCR6020326.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(789..1196)
                     /locus_tag="OO327_003990"
     CDS             complement(789..1196)
                     /locus_tag="OO327_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6020327.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1273..1500
                     /locus_tag="OO327_003991"
     CDS             1273..1500
                     /locus_tag="OO327_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6020328.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1484..1906
                     /locus_tag="OO327_003992"
     CDS             1484..1906
                     /locus_tag="OO327_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020329.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1984..2761
                     /locus_tag="OO327_003993"
                     /pseudo
     CDS             1984..2761
                     /locus_tag="OO327_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2768..3456
                     /locus_tag="OO327_003994"
                     /pseudo
     CDS             2768..3456
                     /locus_tag="OO327_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          885 a          764 c          940 g          867 t
ORIGIN      
        1 ccagaggatt acgaagggct tcataaatat atgctctcac taggattcag gagaacgatc
       61 ccccatggag atggttcgta taatcaactc ccggacggaa cttatgtttc cgaaaagggc
      121 ggtgatattt atcaaattcg cagccagata tctgactatg cagaccgact atccgggtat
      181 cgcgcgtctg tttttgtttg cgaattcagt caatgcgcat ggtatttata ccccgccaag
      241 acccggtgaa tatcctgcac ggccttctgc tttgtagaag gcttcttcgc tatcataatc
      301 gccagattcc agcgcttctt tagccagcca gatgcgtgcc ccagtgcctg catttggctc
      361 cagttgctgg aggcgttttg catcttctag gagtagagcg ataacgtgtt ttaattctgt
      421 ctcgttcatt ttactcacct gaatgtcttc ccaaccaacg acgtgcgcca gcttcggttt
      481 taaacgtttt gcttttggta tacgttatgg cggtgaatgt gccgtcctgg ttggggaaca
      541 cgccgtacac cggagattcg ttgttgccaa gatcgatagt attcatgttg accccatttc
      601 cccttaacgc cggggtagcg gaactgtttg ctgagaacac cgtgcggtgt cttgatgaat
      661 aaaatttaga ataacctaag gagggtgatc aagatttttg tgtagaaaaa cctaagtttt
      721 ttgatgtaaa aaacacaagt gtttgaaagt ttgtgctttt tattacaggg tgtggagaaa
      781 aaaggggatt atttgtttgc gcttcttttg cgagctttga gtagttcttc aaaaagtttg
      841 ttgaaatttt caactcgagc acgcatctct gacaacaggg ccttttgctc tgactcaggc
      901 agtgcgtcga acagttgaag taactctttt tgatcttctg tcagaatggc tggctgatta
      961 tccgggatcg gttcgcctgg ttgtttatct tcatccccaa aaagaagcca agtcggtgag
     1021 cactgaagcg cttggctcag tgcgaataat cttttccccg ccggctgtgt ttcatctctt
     1081 tcccattgag aaattgttac gtgagccact ttgaccagct tacctaatgc ggcctgagac
     1141 agttttaatt ttttacgcct atgtaagagg cgagcaccga aggtttcgtt tttcatatta
     1201 gggaattcta atttttcttg acttaggttt ctctacggcc tggtttcctt aggaaaatct
     1261 aaggagctcg atatgttgaa aattgatgct atagcgtttt ttggcagcaa aacaaagctt
     1321 gccaatgccg caggagttag gctggcaagc attgctgcat ggggggaact ggttcctgaa
     1381 ggtcgcgcga tgcgcctgca agaggcatct ggcggggaac ttcagtacga ccccaaagtt
     1441 tatgacgaat atcgtaaggc aaaacgacct gggaaggtga ttcatgaaaa tcaggcatga
     1501 gcacatcgaa tcagtgctgt tagccctggc agccgaaaaa gggcaggcgt gggtcgctaa
     1561 cgcaattact gaagaatatc tgcggcaggg gggcggcgaa ttgtctctgg taccaggcaa
     1621 ggactggaac aatcagcaga atatctatca ccgttggttg aaaggtgaaa cgaaagcgca
     1681 aagggaaaaa attcagaaac tgatccctgc ggttctggca attcttccgc gtgagctgcg
     1741 tcaccgactc tgtatcttcg ataccctgga acgccgtgca ttactggcgg cgcaggaagc
     1801 gttgagtacg gcaattgatg cgcatgatga tgctgtccag gccgtttacc ggaaagcaca
     1861 tttcagcggt ggtgggtcgc ccggcgattc tgtcgtagtg cattgattga aattaatcgt
     1921 gccggattgt tttgttcggt atcagttaaa tgtaacgctg cgagcgttac aaggtgaaaa
     1981 caaatggctt caaactggat aaagctcgag gttattacgc cggataagcc ggaaatattc
     2041 aggcttgctg agattctgaa tattgatcca gatgccgcat tagggaaggt tattcgcttc
     2101 tgggcatggg cggatcaaca aatgatagac ggtaatgcag attgtaacgc tcgcggcgtt
     2161 acaaaaagtg caatagatcg catcactttt atgtctggtt ttgctgatgc gttaattcag
     2221 gttggatggc tggtcgaaaa tgacggtggg ctttctctac ctaactttga acgtcataac
     2281 ggaaaaagct ctaaaaaacg ggcggttaca aacgagcgag taacaaaaat acgcgaactg
     2341 aaacgaaaag gtaacgctgc cagcgttaca caaacggatc aaaaagcgtt accagtggaa
     2401 gaggaagagg aagatctaaa tactgatctc cccctaaatc cccctcgcca aaaacgagcg
     2461 tctaaaaaat tcgagccgga ggctattgag ctgcccgatt ggttgccgga aacactctgg
     2521 catgagtggg tccggttcag acaggcattg cgaaaaccga ttcgaacgga gcagggcgat
     2581 acgggaactg gaaaaattcc gtcagcaggg ttttacacct gagcaggtga ttcgacacag
     2641 catcgccaat gaataccagg gcctgttcgc gccgaaaggt gttcggcctg agacgttgct
     2701 ccgacaggtt aacaccgtct cgttgccgga cagtgcgatc ccgccaggct tcagggggta
     2761 acggaccatg aaaaatattg cgacaggcgg cgttctggaa cgcatccgcc gactgacccc
     2821 gcaacatgta accgccccat tcagaacggt agcggagtgg cgcgagtggc aacttgctga
     2881 aggccagaaa cgtagcgagg agatcaaccg cctgaatcgc cagttgcggg tggaaaaaat
     2941 tctgaatcgc tcaggcatcc agccgttgca ccgtaaatgc tcgtttgcga attaccaggt
     3001 gcagaacgac ggccagcgat acgcgttaag ccaggcgaaa tccatcgccg atgaactgat
     3061 gaccgggtgt acaaattttg cgttcagcgg aaaacctgat accgggtaga atcacttagc
     3121 ggcagctatc gggaatcgcc tgctgaaaga cggtcagaca gtgattgtgg ttaccgtggc
     3181 tgatgttatg agcgccctgc acgccagcta tgacgacggg cagtcaggcg aaaaattttt
     3241 gcgggagctg tgcgaagtgg atctgctggt tcttgatgaa attggcattc agcgcgagac
     3301 gaaaaacgag caggtggtgc tgcaccagat tgttgatcgc cggacagcgt cgatgcgcag
     3361 cgtggggatg ctgacaaacc tgaactatga ggtgatgaaa acattgctcg gcgagcgggt
     3421 gatggatcgc atggtcatga acggcgggcg ctgggt
//
LOCUS       DAJTSI010000252         3450 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000252 DAJTSI010000000
VERSION     DAJTSI010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.257"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            19..897
                     /locus_tag="OO327_003995"
     CDS             19..897
                     /locus_tag="OO327_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6020330.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            894..2285
                     /locus_tag="OO327_003996"
     CDS             894..2285
                     /locus_tag="OO327_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6020331.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            2282..2539
                     /locus_tag="OO327_003997"
     CDS             2282..2539
                     /locus_tag="OO327_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020332.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            2634..2834
                     /locus_tag="OO327_003998"
     CDS             2634..2834
                     /locus_tag="OO327_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HCR6020333.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            2827..3207
                     /locus_tag="OO327_003999"
     CDS             2827..3207
                     /locus_tag="OO327_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6020334.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
BASE COUNT          906 a          749 c          997 g          798 t
ORIGIN      
        1 cattctgagg tgacagcgat gatgacgttt aacctgcgtg aacaacaaaa aagactacag
       61 gcgcgaatgg atgagttacg ggcagagatt gcatttgctc agaagggcga aaagccatgg
      121 ccttatcgtt cctgcctgat gcgtgaaggt cgcggatatt gcgaaaaaca tggcgaatat
      181 cacacgcata tactggtgtg gagcgatcgt aatggcgagg acagagaaga aatttcatgc
      241 tgccctgact gcttaatcgc tgaggccaac gatttgacca tggagctgtc gtccatcaag
      301 gcggaagagc tgactgataa cgccggaatt gccctgcgtt ttcgggactg cgagtttgat
      361 aattatctgg aggttaatcc tggcgcagcc agaaatcttg cggcctgtcg ccgctatgcg
      421 gagaactggc cagatatgct ggagaacggt accagtcttg ttatgaccgg cagttgtggt
      481 accggaaaaa atcatctggc tgtggctatg gcaaaacaca tcatccgtaa ctatctggcc
      541 agtgtggaga tcaccgacgt gatgcgcctt acccgtgctg tgaaaaattg ctggcggaat
      601 gacagcgaaa aaacagcgga tgaagttatt gagcgttatg cgtcaatgga tttgctgatc
      661 atcgacgaag ttggcgttca gtttggcagc gcggctgaaa tggctatttt gcaggaaatt
      721 atcaacgcca ggtacgaaag tatcttgccc accattctga tcagtaacct ctcaccggaa
      781 gagttgtggg cgttcatcag tccccgaatt gccgacagga tcacagacgg gggacgcaac
      841 tggttgtcgt ttaactggcc cagttaccgt tctcgtatca gaggtgtggc tgcatgacaa
      901 ctccagtatg gcgtaacgat gaccttgaag gcgctgtcat cggcgcgttc tttctgcgcg
      961 gagcagatcc tgaagtgatg gatattctgg ccacactgcc agcggacgtt ttttctgtac
     1021 gagcgtatca ggatatctat acaggcatct gcagacaggc ccgtgtttca ggagtgattg
     1081 accccgtgct gttgtgtaat gagatgccgg aacttgcccc ggtgattact gataccgggc
     1141 gtaaaacctg ggtgaagtct tcactggagc actatgttgc agcgttgcgg cgcaatgccg
     1201 cactgcgcga tgcagaaaaa acactgaatg aggcgctgca gaaattacgt gatgcgcata
     1261 cctgtgaagc agctgaagat gccctgaagg atgcgcagaa catgatggtc acactgtcga
     1321 caggaaaggg cgtcattcag ccggtacata ttgatgatgt gcttccggaa gtggttgagc
     1381 gtgttgaatg ccggaatcag gggctggaga aatccaggac gttgatgacc ggtattgatg
     1441 aactggacgc aaaaacaggc ggtatggagc ccggagacct ggtattcatt gccgcccgtc
     1501 cttcgatggg gaaaaccgaa cttgcgctgg acatcatcga caaggtgact gagcaggggc
     1561 atggtgtgct tctgttcacc atggagatgg cgaacatcca gattggtgaa cgtatggtgt
     1621 ctgcggctgg agggatgcca gtatcacgcc tgaaatctgt ggctcacttt gaagatgaag
     1681 actggacgcg tttctcacaa ggggtggggc ggatgaccgg gcgcaatatc tggatggtgg
     1741 accaggcgaa cctggccatt gatgagatat gcgcaacaac gaaacaccac ctgattaaat
     1801 atccggaaac ggcgctggtg gtggttgatt atctcgggct gataaaaacc cgaaccacgg
     1861 ggcgtcatga ccttgccgtg ggtgaaatct caaaggggct taaaggcctg gcaaaatccg
     1921 gtggttttcc gttgattgcg ctgagccagc tctcccgcgg tgtggagtcc agacccaata
     1981 aacgtcccat gaactcagac ctgaaaaatt ccggagaaat agaggcggat gcagacatca
     2041 ttctgatgct ttacagggat gaagtgtaca acccggatac gcaggccagg ggcatcgcag
     2101 aaatcaatat cacgaaacaa cgtaacggtt ctctgggaac gatttaccgg cgtttttata
     2161 acggacattt tctgcctgtg gaccaggaaa gtgcacgggt gctttccact cccatgaagc
     2221 ccggcaatcc gcgcagatac agtaataaac gaactgacag cagtaagatg gagcgtttct
     2281 tttgaacaac cagacaatga cttttacccc tgaacaattg cgtaaacacg cgcaggaaat
     2341 gctgcgtcat gctgaacaac tcgaaaaaac gggcataaca aaagatgcta tccgtaaaga
     2401 tatggtaccc gcgcttcggg aactgatgca ggcgaagcat cgcgcacaga aagcagtaga
     2461 tgagctggtg gactgtgtgg cagagctgga aaccagagtc ggaaagtttg aaaaactggt
     2521 gcaggaggcg ctgcgctgat gcgccatgag tttgttttgc cctggcttgt gcgcgatcat
     2581 tatgtccctg gtggaaagcc cgggataaaa atcaccgaac cggagagtat ttgatgagcg
     2641 tcaaaattca aacgataccg gaactgctta tccagacacg aggtaatatg accgaagttt
     2701 cacgaatgct gaactgtaat cgcgccacgg taagaaaata tgctgaagat aaagagggca
     2761 aagggcacgc cattgttgat ggtgttctga ttgttcatcg tggatgggac aggggtaaag
     2821 acagtgatgc gtgatattca gcaggttctt gagcgctggg gcgcgtgggt ggctaataat
     2881 catgaggatg tgacctggtc gtccattgct gccggtttta agggattaat cccttcaaaa
     2941 gtaaaatccc gcccgcaatg ctgtgacgat gacgcgatgg ttatatgtgg gtgtatggcc
     3001 cgccttaacc ggaacaacaa cgatctacat gacttgctgg ttgactacta cgtgtttggg
     3061 atgacattca tgacgctggc gcggaagcat ggatgctcag atggttatat aggaaagaag
     3121 ctacaaaaag ccgagggggt ggtggaagga atgttgatga tgttgggtgt ccggttagaa
     3181 atggacaaat atattgaaca tccctgatgg taaggggcga ctttctagaa aatattttac
     3241 gtacgtaaaa aacgggatat tctgttaaga gtggttgctt cgccacacag cttaaacccg
     3301 cctccgggcg ggttttttat gcctgaaaaa cggtacagga cgttaaacac gctggtggtc
     3361 agatgagttt gcagatgtga tgacatatgg ttattattct gcctccggcc ctttagctca
     3421 gttggtcaga gcgagcgact cataatcgcc
//
LOCUS       DAJTSI010000253         3413 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000253 DAJTSI010000000
VERSION     DAJTSI010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.258"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..237
                     /locus_tag="OO327_004000"
     CDS             <1..237
                     /locus_tag="OO327_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCR6020335.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            247..885
                     /locus_tag="OO327_004001"
     CDS             247..885
                     /locus_tag="OO327_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR6020336.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            888..2051
                     /locus_tag="OO327_004002"
     CDS             888..2051
                     /locus_tag="OO327_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6020337.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2135..>3413
                     /locus_tag="OO327_004003"
     CDS             2135..>3413
                     /locus_tag="OO327_004003"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR6020338.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPVTMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEG"
BASE COUNT          759 a          803 c          987 g          864 t
ORIGIN      
        1 ttgttaggtt acgttatccc attatccagt gcggcaatca atgcggtgag tattccagac
       61 tatttcgcct ggggcgggat tgcactggtg attcagttac tcgtttttgc tggtgtcaga
      121 ctttatatgc ccgcattaag cgaaaaaatt attaatcaca ataccgcagc aggaatgttt
      181 atgggaaccg ccgcgctggc tggcggtatt tttaacgcag cttgtatgac atggtaatgg
      241 acgatcatgg ccagaaaacg caaatcaaga aataacagta aaattggtca cggagcgatc
      301 agtcgcattg gtagaccgaa taatcctttt gaaccgcgtc gcaatcgcta cgcacaaaaa
      361 tacttaacgt tggcgctaat gggcggtgcc gcttttttcg tattgaaagg ttgtagcgat
      421 agtagcgatg tcgataacga cggcgacgga acattttacg cgacagtgca ggattgtatt
      481 gatgacggta ataatgccga catttgcgcg cgtggctgga acaacgccaa agcggcattt
      541 tatgccgatg ttccaaagaa tatgactcag cagaactgtc agtctaagta cgaaaattgc
      601 tactacgaca atgttgaaca gagttggatc ccggtcgttt ctggattttt gttaagccgg
      661 gttattcgta aagatcgcga tgagccgttc gtctataaca gcggcggatc ctcatttgct
      721 tcccgccccg tctggcgcaa cacttccggt gattactcct ggcgcttcgg ttctggcaaa
      781 aaagagtctt actcttcagg cggctttacc accagaaaag cgtcgaccgt ttcgcgcggc
      841 ggctatggtc gttcttccag cgcccgtggg cattggggag gctaatcatg ctgagacaca
      901 acgttcctgt gcggcgggat ctggaccaga tcgccgctaa caacggtttc gactttcata
      961 tcatcgacaa tgaaatctat tgggacgaga gtcgggctta ccgttttact ctgcgccaga
     1021 ttgaagagca gatcgaaaaa ccgactgcgg aactgcatca gatgtgcctt gaggtggtgg
     1081 atcgcgcggt taaagacgaa gagatcctga cgcaactggc gatcccgccg ttgtactggg
     1141 atgtgatcgc cgaaagctgg cgcgcacgcg atccttcgct gtatggccgg atggattttg
     1201 cctggtgtgg caatgcgccg gtgaagctgc tggagtacaa cgccgatacg ccgacctcgt
     1261 tgtatgagtc ggcttatttc cagtggttgt ggctggagga tgcccggcgc agcggcatta
     1321 ttccgcgtga tgccgatcag tacaacgcca ttcaggaacg cctgatttcg cgctttagtg
     1381 agctttacag tcgggaactg ttttattttt gctgctgtca ggacaccgat gaagacagga
     1441 gtaccgtgct gtacttgcag gactgcgccc agcaggcagg gcaggagtcg cggtttatct
     1501 acattgaaga tctcggtttg ggcgtcggcg gcgtactgac cgatcttgat gataatgtca
     1561 tccagcgtgc atttaagctg tatccgctgg agtggatgat gcgtgacgat aacggtccgc
     1621 tgctgcgcaa gcgtcgcgag caatgggtgg agccgttatg gaaaagtatc ttgagtaata
     1681 aagggctaat gccgctgctt tggcgcttct tccctggtca tcctaacctt cttgcatcct
     1741 ggtttgaggg tgaaaaatcg cagattgccg ctggcgaaag ctatgtgcgt aaaccgatct
     1801 actcgcgcga aggcggaaac gtcaccattt tcgacggtca gaataacgtc gttgaccacg
     1861 ctgatggtga ttacgccgat gaaccgatga tctaccaggc gtttcaacct ctgccgcgat
     1921 ttggcgatag ctacacactc atcggtagct ggattgtcga tgatgaagcg tgcggaatgg
     1981 ggatccgtga agataacaca ctgatcacca aagacacctc acgtttcgtt ccgcattaca
     2041 ttgctggata agaatgtttt agcaatctct ttctgtcatg aatccatggc agtgaccata
     2101 ctaatggtga ctgccattga tggagggaga cacagtgcac tggcaaactc acaccatttt
     2161 taatcaacct ataccattaa ataacagcaa cttatacctg tctgatggcg cgctttgcga
     2221 agcggtaacg cgtgaaggtg ctggctggga tagcgatttt ctagccagta ttggtcagca
     2281 gttaggaacg gctgaatccc ttgaactggg gcggctggcg aatgtaaacc cgcctgaact
     2341 gttgcgctac gatgcccagg ggcggcgtct ggacgatgtt cgtttccatc ccgcatggca
     2401 cctgctgatg caggcgttat gtaccaatcg ggtgcacaat cttgcctggg aagaagacgc
     2461 tcgctccggc gcatttgtgg cgcgcgcggc gcgttttatg ttacatgcgc aggttgaggc
     2521 tgggtcgtta tgtccagtaa cgatgacctt tgccgccacg ccattgttgt tacagatgtt
     2581 acccacgccg tttcaggact ggaccacgcc gctgctgagc gatcgctacg attctcactt
     2641 attgccaggt gggcaaaaac gcggtttgtt gattggcatg ggaatgacgg aaaagcaggg
     2701 cggttccgat gtcatgagca acaccacccg tgcagagcgt ttggaagatg gctcttatcg
     2761 gctggtgggg cataaatggt ttttctcggt gccgcaaagc gatgcgcatc tggtgctggc
     2821 gcagaccgcg ggcggtctgt cctgcttttt tgtgccgcgc tttttgcctg acgggcaacg
     2881 caacgcgatt cgcctcgagc ggctgaaaga taagctgggt aatcgctcta acgccagttg
     2941 cgaagtggag tttcaggatg ccattggctg gttattgggg caggaagggg aaggaattcg
     3001 tctgatcctg aaaatggggg ggatgacgcg ttttgattgc gccctgggta gccatgccat
     3061 gatgcgccgt gcattttcgc tggcgattta tcatgcacat caacgccatg tttttggtaa
     3121 tccattgatc caacagcccc ttatgcgtca tgtcttaagt cgcatggcac ttcagcttga
     3181 agggcaaacg gcgttgctgt ttcgtcttgc gcgagcgtgg gaccggcgtg ccgatgccaa
     3241 agaagccctg tgggcgcgtt tatttacgcc tgcggcaaaa tttgtgatct gcaaacgagg
     3301 tatgccgttt gtggccgaag cgatggaggt gctgggcggc attggttatt gcgaggagag
     3361 cgagctgccg cggctttacc gggagatgcc ggtaaacagt atttgggaag gtt
//
LOCUS       DAJTSI010000254         3412 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000254 DAJTSI010000000
VERSION     DAJTSI010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.259"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(68..592)
                     /gene="sat2"
                     /locus_tag="OO327_004004"
     CDS             complement(68..592)
                     /gene="sat2"
                     /locus_tag="OO327_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR6020339.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(687..1160)
                     /locus_tag="OO327_004005"
     CDS             complement(687..1160)
                     /locus_tag="OO327_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR6020340.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1492..2028
                     /locus_tag="OO327_004006"
     CDS             1492..2028
                     /locus_tag="OO327_004006"
                     /inference="COORDINATES: protein motif:HMM:NF024886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HCR6020341.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIK"
     gene            2143..2469
                     /locus_tag="OO327_004007"
     CDS             2143..2469
                     /locus_tag="OO327_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6020342.1"
                     /translation="MFVFPSSTLCNHPYNGKLCRHHLHDSVARKALKAAVQKAGIVSK
                     RVTCHTFRHSFATHLLQAGRDIRTVQELLGHNDVKTTQIYTHVLGQHFAGTTSPADGL
                     MLLINQ"
     gene            complement(2657..2896)
                     /locus_tag="OO327_004008"
     CDS             complement(2657..2896)
                     /locus_tag="OO327_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCR6020343.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT          943 a          756 c          720 g          993 t
ORIGIN      
        1 cttccctcat gatgtttaac tcctgaatta agccgcgccg cgaagcggtg tcggcttgaa
       61 tgaattgtta ggcgtcatcc tgtgctcccg agaaccagta ccagtacatc gctgtttcgt
      121 tcgagacttg aggtctagtt ttatacgtga acaggtcaat gccgccgaga gtaaagccac
      181 attttgcgta caaattgcag gcaggtacat tgttcgtttg tgtctctaat cgtatgccaa
      241 ggagctgtct gcttagtgcc cactttttcg caaattcgat gagactgtgc gcgactcctt
      301 tgcctcggtg cgtgtgcgac acaacaatgt gttcgataga ggctagatcg ttccatgttg
      361 agttgagttc aatcttcccg acaagctctt ggtcgatgaa tgcgccatag caagcagagt
      421 cttcatcaga gtcatcatcc gagatgtaat ccttccggta ggggctcaca cttctggtag
      481 atagttcaaa gccttggtcg gataggtgca catcgaacac ttcacgaaca atgaaatggt
      541 tctcagcatc caatgtttcc gccacctgct cagggatcac cgaaatcttc atatgacgcc
      601 taacgcctgg cacagcggat cgcaaacctg gcgcggcttt tggtacaaaa ggcgtgacag
      661 gtttgcgaat ccgttgctgc cacttgttaa cccttttgcc agatttggta actataattt
      721 atgttagagg cgaagtcttg ggtaaaaact ggcctaaaat tgctggggat ttcaggaaag
      781 taaacatcac cttccggctc gatgtctatt gtagatatat gtagtgtatc tacttgatcg
      841 atcaggcttt tgtatatctc cccaccacct gaaacaatga catgatccgt tattttcttt
      901 aggttggtta aagcatcttt aattgatgga aagatcaata cgttctcatt gtcagatgta
      961 aaacttgaac gtgttacgac cgcatacttt cggttgggta atgctcccat tgattcaaaa
     1021 gtcttgcgtc caaccaacag ccattggtta taggtaatag ctttaaacag gagctgttca
     1081 cctttggcac tccatggaat atcagggcca ttcccgataa ctccattctt cgatatagct
     1141 accattagtg atagtttcac aattcttcct cagaggttaa cattttatta gtaagcatgc
     1201 tcgttttgtc acccgctgat gcttaccgtt aattaatata atcacttgta ttaaatagac
     1261 tttctttaaa aatacaagac actctgttat tacaaatcgt gcatgccgtc tatcctgctt
     1321 gcacgatgca cattatctca cccaaagaac tcataacatt ttaatttatt tagcattaaa
     1381 ttaaaactct accggtaaac aagcatctct aggcgtaaaa aacaggcact tttttaaatc
     1441 ccagctagaa taggctgtat aggcagacag ttgcaagaca aggagcgttt tatgtctaac
     1501 agtccatttt taaattctat acgcacggat atgcgacaaa aaggttatgc gctgaaaact
     1561 gaaaaaactt acctgcactg gattaagcgt tttattctgt ttcacaaaaa acgtcatcct
     1621 cagaccatgg gcagtgaaga ggtcaggctg tttttatcca gcttagcaaa cagcagacat
     1681 gtagccataa acacgcagaa aatcgcttta aatgccctag cttttttgta caacaggttt
     1741 ttacaacagc cgttgggcga tattgattat atccctgcaa gcaagcctag acggctaccc
     1801 tctgttatct ctgcaaatga agtgcaacgc attttgcagg ttatggatac tcgcaaccaa
     1861 gttattttta cgctgctgta tggtgcaggt ttgcgcatta atgaatgctt gcgtttgcgg
     1921 gttaaagatt ttgattttga taatggctgc atcactgtgc atgacggtaa gggtgggaaa
     1981 agcagaaaca gcctactgcc cacgcgccta atcccagcaa taaaataact cattgagcaa
     2041 gcgcggctta ttcagcaaga cgacaactta caaggcgtag ggccatcgct gccttttgct
     2101 ttagatcaca aatacccttc tgcttatcga caagcggcgt ggatgtttgt ctttccctcc
     2161 agcacgctct gcaaccaccc gtataacggc aaattatgcc gccatcatct gcatgactcc
     2221 gttgcgcgaa aggcattgaa ggcagccgta caaaaagcag gcatcgttag caagcgtgtc
     2281 acttgtcata catttcgtca ctcgtttgct acgcatctat tacaagcggg gcgtgatatt
     2341 cgcactgtgc aagaactctt agggcataac gatgttaaga ccacgcaaat ctatacgcat
     2401 gtgttgggtc agcattttgc cggcaccacc agtcctgcgg atggactgat gctacttatc
     2461 aatcagtaaa agtagataaa tatctttatg tagccctgag tagcccctga tactttttat
     2521 ggtgaaaaag ataaacaatc atagagggga taaatatgag ccagttctac cattgttcag
     2581 ataagtttaa gcaagatact gtcaatcaca gtgaccattc atggtcatta cacttcctcc
     2641 agttgccaga caatgcctaa cataaaaacg caaaagccca aaagtcagag cttaggtgtt
     2701 ctaggcgttt tctaagcgct gaagaaatac gtcttaccgt agattgcgtg cgtttctgaa
     2761 gcacattttc cttatacatg gctttatccc aggcagcttc atcaagctct tggagtaaca
     2821 agccagtaat aatgcgacac tctcgggttt gtagcgagcc gctggtgaga tcggagttat
     2881 agcggaaagg ggtcatttgt aattatttgt gggtggagga tagttaactt attattgtag
     2941 ttggaagagg ctgctttcca aacattagca cctcgtaatg cttgttactg tatattaatt
     3001 tatgctctta gcgcatgtta atgggctatt acaatgcata atgtccacat caagataact
     3061 gtggcttaac acctgtacaa gcgaaaaaaa tcaatcacct gtccggaaat acgggatcac
     3121 tgtaaagtgt tggtgaggca gtctaggagg gggatttaat ttgggatact gatgaaagga
     3181 ttatgatcag ttggatatag gatcgtacta agctgagcgg caggttagct agattagtta
     3241 accagataag tgaaatctag ttccaaacta ttttgtcatt tttaattttc gtattagctt
     3301 acgacgctac acccagttcc catctatttt gtcactcttc cctaaataat ccttaaaaac
     3361 tccatttcca cccctcccag ttcccaacta ttttgtccgc ccacagctgc cg
//
LOCUS       DAJTSI010000255         3383 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000255 DAJTSI010000000
VERSION     DAJTSI010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.260"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1742
                     /gene="fepA"
                     /locus_tag="OO327_004009"
     CDS             <1..1742
                     /gene="fepA"
                     /locus_tag="OO327_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCR6020344.1"
                     /translation="TKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSF
                     RLYGNLDKTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSL
                     ELEAGYSRQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSN
                     WVQYEHTRNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTL
                     TLGTEWNQQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELT
                     DSTIVTPGLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYI
                     LYSKGQGCYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIE
                     AGYVAVGQNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTT
                     GDRLSIIPEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISP
                     YSIVGLSATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNE
                     PGRTWYMSINTHF"
     gene            1917..2537
                     /gene="entD"
                     /locus_tag="OO327_004010"
     CDS             1917..2537
                     /gene="entD"
                     /locus_tag="OO327_004010"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCR6020345.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(2659..2811)
                     /gene="hokE"
                     /locus_tag="OO327_004011"
     CDS             complement(2659..2811)
                     /gene="hokE"
                     /locus_tag="OO327_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR6020346.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(3162..3314)
                     /gene="hokE"
                     /locus_tag="OO327_004012"
     CDS             complement(3162..3314)
                     /gene="hokE"
                     /locus_tag="OO327_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR6020347.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLAGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
BASE COUNT          904 a          872 c          872 g          735 t
ORIGIN      
        1 ttaccaaaaa aggcagcggc gagtggcacg gctcctggga cgcatatttc aatgcgccag
       61 aacataaaga ggaaggtgcc accaaacgca ctaactttag cctgaccggt ccgctgggcg
      121 acgaattcag cttccgcttg tatggcaacc tcgacaaaac tcaggctgac gcgtgggata
      181 tcaaccaggg ccatcagtcc gcgcgtgccg gaacgtatgc cacgacgtta ccagccgggc
      241 gcgaaggggt gatcaataaa gatattaacg gcgtggtgcg ctgggacttc gcaccattgc
      301 aatcgctgga actggaagcg ggttacagcc gccagggtaa cctgtatgcg ggcgataccc
      361 agaacaccaa ctctgacgct tacactcgct cgaaatatgg cgatgaaacc aaccgcctgt
      421 atcgccagaa ctacgcgctg acctggaacg gtggctggga taacggcgtg accaccagca
      481 actgggtgca gtatgaacac acccgtaact cgcgtattcc ggaaggtctg gcgggcggta
      541 ccgaagggaa atttaacgaa aaagcgacac aggatttcgt cgatatcgat ctcgatgacg
      601 tgatgctgca cagcgaagtt aacctgccga ttgatttcct cgttaaccag acgctgacgc
      661 tgggtacgga gtggaatcag caacggatga aggacttaag ttccaacacc caagcactga
      721 ccggaacgaa taccggtggc gctattgatg gcgtgagtgc caccgaccgt agcccgtatt
      781 caaaagcaga aattttctcg ctgtttgccg aaaacaacat ggagctgact gacagcacca
      841 tcgtaacgcc ggggctgcgt ttcgatcatc acagtattgt cggcaataac tggagcccgg
      901 cgctgaacat atcgcaaggt ttaggcgatg acttcacgct gaaaataggc attgcccgcg
      961 cctataaagc gccgagccta taccagacta acccgaacta cattctctac agtaaaggtc
     1021 agggctgcta tgccagcgcg ggcggctgct atctgcaagg taacgatgac ctgaaagtag
     1081 aaaccagcat caacaaagag attggtctgg agttcaaacg cgacggctgg ctggcgggcg
     1141 tcacctggtt ccgtaacgat tatcgcaata agattgaagc gggctatgtg gctgtagggc
     1201 aaaacgcagt cggcactgat ctctatcagt gggataacgt gccgaaagca gtggttgaag
     1261 gtctggaagg atcgttaaac gtaccagtta gcgaaacggt gatgtggacc aataacatca
     1321 cttatatgct gaagagtgaa aacaaaacca cgggcgaccg tttgtcgatc atcccggagt
     1381 acacgttgaa ctcaacgctg agctggcagg cacgggaaga tttgtcgatg caaacgacct
     1441 tcacctggta cggcaagcag cagccgaaga agtacaacta taaaggtcag ccagcggttg
     1501 gcccggaaac caaagaaatc agtccttaca gcattgtggg tctgagcgcg acctgggatg
     1561 tgacgaagaa tgtcagtctg accggcggcg tggacaacct gttcgacaaa cgtttgtggc
     1621 gtgcaggtaa tgcccagacc acgggcgatc tggcaggagc caactatatc gccggtgccg
     1681 gcgcgtatac ctataacgag ccgggacgta cgtggtatat gagcattaat actcacttct
     1741 gatgctaacg tcagattgtt gacaaagtgc gcgtcgttca cgccggatgc ggcgtgaacg
     1801 ccttatccgg cctacaaaga tcttgccaat tcaatatatt gcaggatcac gtaggcctga
     1861 taagcgtagc gcatcaggca gttttgcgtt tgtcatcagt ctcgaatatg gtcgatatga
     1921 aaactacgca tacctccctc ccctttgccg gacatacgct gcattttgtt gagttcgatc
     1981 cggcgaattt ttgtgagcag gatttactct ggctgccgca ctacgcacaa ctgcaacacg
     2041 ctggacgtaa acgtaaaaca gagcatttag ccggacggat cgctgctgtt tatgccttgc
     2101 gggaatatgg ctataaatgt gtgcccgcaa tcggcgagct acgccaaccg gtgtggcctg
     2161 cggaggtata cggcagtatt agccactgcg gggctacggc attggccgtg gtttcccgtc
     2221 aaccgattgg cgttgatatc gaagaaattt tttctgcgca aacagcgacc gaattgacag
     2281 acaacattat tacaccagca gaacacgagc gactcgcaga ctgcggttta gccttttctc
     2341 tggcgctgac acttgcattt tccgccaaag agagcgcatt taaggcaagt gagatccaaa
     2401 ctgatgcagg ttttctggac tatcagataa ttagctggaa taaacagcag gtcatcattc
     2461 atcgtgagaa tgagatgttt gctgtgcact ggcagataaa agaaaaaata gtcataactc
     2521 tgtgccaaca cgattaattg acaacatctg gtacgattcg cccgcagcca tcactgactg
     2581 cgggcgaaag tgtaaagcag gtgccttacc actctgacct gacaaccgga tatgcgggga
     2641 ttactccccg cacaacggct acttcttcgg ttcgtaagca agaacagcct taaactcaat
     2701 attacgttcc tttacggtga actcacacag cgagtctccg accagaagcg taaatcccag
     2761 caccgtcaaa cacagcacta tgactgccac aagggcatat ttcgtcagca tgtttatatt
     2821 gcctccatgc gaagaaggga ctaccatccg agttgttgag gttcggagtg gcagccccag
     2881 gattgataga aatatctctt ggggcttcat ctttccaaac ctcagagtta acctgaaacc
     2941 ggaaagcttc aggcacccgc cgttatctta cccttcctta gcaaaatagc tgtttattta
     3001 tacagtgagt cctgtttcgg acttctgtcg gcaaaactca tcattgctct taaaacgaaa
     3061 tcgcccgcag ccatcactga ccgcgggcga aagtgtaaag caggtgcctt accatcctga
     3121 cctgacaacc ggatatgcgg ggattactcc ccgcacaacg gctacttctt cggttcgtaa
     3181 gcgagaacag ccctgaactc aatattacgt tccttcaccg taaactcaca cagcgagtct
     3241 ccggccagaa gcgtaaatcc cagtaccgtc aaacacagca ctatgactgc cacaagggca
     3301 tatttcgtca gcatgtttat attacctcca tgcgaagaag ggactaccat ccaaattgtc
     3361 tggtttggag tggcagcccc agg
//
LOCUS       DAJTSI010000256         3374 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000256 DAJTSI010000000
VERSION     DAJTSI010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.261"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..861
                     /gene="msrP"
                     /locus_tag="OO327_004013"
                     /pseudo
     CDS             1..861
                     /gene="msrP"
                     /locus_tag="OO327_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            862..1497
                     /gene="msrQ"
                     /locus_tag="OO327_004014"
     CDS             862..1497
                     /gene="msrQ"
                     /locus_tag="OO327_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCR6020348.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            1755..2405
                     /gene="zinT"
                     /locus_tag="OO327_004015"
     CDS             1755..2405
                     /gene="zinT"
                     /locus_tag="OO327_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR6020349.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT          940 a          760 c          740 g          934 t
ORIGIN      
        1 tggtttaaag ggaacgatcg tccgcccgcc cccgccggaa aaccgctgga gttcagcaag
       61 cctgccgcct agcaaaataa cctgccactg acgccagcag ataaagtctc cggttataac
      121 aacttctatg aattcgggct ggataaagac gatcccgcgg ctaatgctgg tagcctgaaa
      181 accgatccat ggacactgaa aatcagcggc gaagtggcaa aaccattgac cctcgatcat
      241 gatgatttaa cccgtcgctt cccgctggaa gagcgtattt atcgtatgcg ctgcgtggaa
      301 gcgtggtcga tggtggtgcc gtggattggt tttccgctac acaaattgct ggcgcttgcc
      361 gaacccacca gcaatgcgaa gtatgtcgct ttcgaaacga tttatgcgcc ggaacagatg
      421 cctggccagc aggaccgctt tatcggcggc gggctgaaat atccttatgt cgaaggattg
      481 cgtctcgacg aagcaatgca tccgctcaca ctgatgaccg taggtgttta tggcaaggcg
      541 ttaccaccgc aaaatggcgc gccggtgcga ctgattgtgc cgtggaaata tggctttaaa
      601 gggattaaat cgatcgtcag tattaagctg acccgcgagc gtccgccaac cacctggaat
      661 ctggcagcgc ctgacgaata cggtttttac gccaacgtta atccgcatgt tgatcacccg
      721 cgctggtcac aggctaccga acgatttatt ggttcaggcg gcattctcga tgttcagcgc
      781 cagccaacac tactgtttaa tggttacgcc gaccaggtgg catcgctgta tcgtggcctg
      841 gatttgcggg agaatttcta aatgcgtctg acagcaaaac aagtgatatg gctgaaagtt
      901 tgcctgcatc ttgccggatt gttgccgttt ctctggctgg tctgggcgat caatcacggt
      961 ggactgggtg ccgatccggt gaaagatatt cagcatttta ccggtcgcac tgcactgaaa
     1021 tttttgcttg cggccttgtt aatcacacct ctggcacgct acgcaaaaca gccgttattg
     1081 atacgcactc gccgcctgtt aggcttatgg tgctttgcct gggcgacact gcatttaacc
     1141 agttacgcat tgctggagtt gggcgtgaat aatctggcgt tattaggtaa ggagttaatt
     1201 acccggcctt atttaacgtt aggaattatc agctgggtta ttttgcttgc tttagcgttc
     1261 acttcaaccc agtcgatgca gcgaaaactc ggcaaacact ggcaacagtt gcataacttc
     1321 gtctatcttg tcgcgatcct ggccccaata cattatctgt ggtcagtgaa gattatttca
     1381 ccgcagccac tcatctacgc cgggctggct gtactgcttt tagccttacg gtataagaag
     1441 ttgctttccc ttttcaaccg gttacgcaag caggcccaca acaaattatc tctataactt
     1501 catattgccg acaaacttcg cgttatcccg gccggatgcg gcgcgagcgc cctatccggc
     1561 ccacacagat cgcatcaagt tatgtttcgt ttgtcatcaa cctttcatca ccagaataat
     1621 ttattttatt tcaataagat agttcaatac acctgagaat accatgctct catttcctaa
     1681 tgagttgttg cattttttca tatgttacaa tataacatta cacataatgt acattaactc
     1741 tggaggaaat tgttttggcg attcgtcttc ataaactggc tgttgcttta ggtgtcttta
     1801 ttgttagcgc tcctgccttt tcgcatggtc atcattctca tggcaaaccc ttaacagagg
     1861 tcgaacaaaa agctgctaat ggtgtttttg atgataccaa tgtacaaaac cgaacgctca
     1921 gtgactggga tggagtctgg caatccgttt accctttact gcaaagtggc aaacttgatc
     1981 ccgtctttca gaagaaagcg gatgcagata aaaccaaaac atttgctgaa attaaagatt
     2041 attatcgcaa aggttatccg acagatatcg aaatgattgg cattgaggac ggcattgtcg
     2101 aattccatag aaataatgaa acaacatcct gtaaatatga ttacgatgga tacaaaatac
     2161 tcacctataa atcaggcaag aaaggcgttc gttacttatt cgaatgtaaa gatcctgaaa
     2221 gcaaagcccc taaatatata caatttagcg atcatataat tgcaccacga aaatcatctc
     2281 attttcacat ttttatgggt aatgattccc agcaatcttt attgaatgaa atggaaaact
     2341 ggccaacgta ttatccctat cagttgagta gcgaagaagt ggtcgaggag atgatgtctc
     2401 attgattttt ttgcgctgac ttcagttatt taacagaaca gtgcctggta gtaacaacaa
     2461 taatttacca agacaaataa gtaagacaaa ctcagacacg ctcatacttg agtcgtgtct
     2521 tttttattat gtctgtagaa aaaaactgag aaaaaggcta acaataaata cacaactcag
     2581 ccatacagac tggcagcgca aatgtttcca ggagcaagtc atctgtcatg gtaaagctaa
     2641 ccagaaatat aggttaattt tggtgtttaa acctttttta cttgttagta tgatacaaag
     2701 gctttcaaaa aagctgggaa attcaacaac tcttggaaat tatggacttt gtcccgcggc
     2761 tttaatctga cggccgcgtt ccttttttgg ctcaattatt gaccccttct ctacgcaact
     2821 tcagttcccg ccagcaactt tgcggcagtt tcataggatc aatgtctaaa agaataatgg
     2881 tgaccgataa gaaaacgact gaataactgt agattttcgc tcgaaacctt cctgtcagct
     2941 ccatagcgaa tcaagcgctg aatgtcacag tatcgaacag aaaacagtga cgatctaacc
     3001 cttcaagaat attctacgat tgttctgttt aggaaaagca aggcgggaag tcgggagata
     3061 agtcattgat aaaatggcgg agagaggggg atttgaaccc ccggtagagt tgcccctact
     3121 ccggttttcg agaccgatga tacagagtcg aaaaatcaaa gagttaaaaa tttttcttgg
     3181 aaaaaatgtc ttaaaatcgt acacgaaaaa tcaatgaatt acataacatt aaagaagaat
     3241 attccaagcc ttcttgagtc caccacctgt tttcaggtgt cattcagacg tgatttgccc
     3301 cagattggga aagcacgctt ttttctcagc ttattccgtt ttgcctgtgt gttgaataat
     3361 tccactgcag ttat
//
LOCUS       DAJTSI010000257         3361 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000257 DAJTSI010000000
VERSION     DAJTSI010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.262"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(627..1043)
                     /locus_tag="OO327_004016"
     CDS             complement(627..1043)
                     /locus_tag="OO327_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020350.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1070..1210
                     /locus_tag="OO327_004017"
                     /pseudo
     CDS             1070..1210
                     /locus_tag="OO327_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1210..2235
                     /locus_tag="OO327_004018"
     CDS             1210..2235
                     /locus_tag="OO327_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6020351.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(2288..2377)
                     /locus_tag="OO327_004019"
     tRNA            complement(2288..2377)
                     /locus_tag="OO327_004019"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2341..2343),aa:Ser,seq:cga)
     gene            2471..3268
                     /gene="mtfA"
                     /locus_tag="OO327_004020"
     CDS             2471..3268
                     /gene="mtfA"
                     /locus_tag="OO327_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR6020352.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
BASE COUNT          967 a          747 c          719 g          928 t
ORIGIN      
        1 acgtaagagg ctaacctaca tgtgttcagc atggattgag cctcagatta atgttaagcg
       61 tcttgcagga cgcttaatgt taactggggc ttttctctat ctgccttttg gtgttcatgc
      121 ctgagacaga tagcctcaag cacccgcagt cattctactt aactaagatt tccctgcaaa
      181 ccgtttttgt ccggcacagt aaatatccaa ctaaaccaat agcgttcgct gtatttaccg
      241 ccagtattca atgcacatga ccgccatgaa cacccctaaa aaaagggcat ttatatgtcc
      301 aaacattaat atcaaaacat caattttttc catatacctt gctgtgaaga tgatgggcat
      361 acatgatgcg aacaaccaga acgcaacaaa caaaaactgc aatgcgtttt tcattattcc
      421 ccctacaatc aatgtgcaat aacatttaaa cacacctcaa tttggccgga catataaata
      481 tctaaaccag aaaaaatcac ttacatagcg ttacaaactc tttagtctaa atactcatcg
      541 taaaacattc cccatactta tcagtccgtt ccgcgccagg tagcttattg ccttatctgg
      601 caacctataa tcaggtttcc gctttttcag ttggctggtc gtttaaccga catagttaac
      661 ccattaatct ggttgccgga tgctggtgga ttttcgcgtt ttaattgttc ataaaagtgc
      721 acagctttaa ccagttcttc tgatgtaacc gggactggtg gggcagtgaa taaggcctga
      781 atttcatagt tcggcctgtc gttacaatcc tcttttttcg gtacatattt ccagtcacca
      841 acccacaact ccccctgaga gtccataaca ccttttttca cgtagcgata tcgccacgct
      901 atcggctctg cttccagcga tgcccgtgca attttgaata actcgccctc tactcgcgcc
      961 atccctgaat tggggtggca tttcgtaatc gctattttta atttggcttc ttcgattaat
     1021 tgttcttttg ttaattcagt cattttcatt accgccctgg taatgctgcc accagcgaaa
     1081 atggttaatg gaaaacgggc ttccgttcta taccaatcgt agtggcaaac cgattgtcag
     1141 ccgggatcta tttacctgca ataaaacttt accaccacgc gaggtagagc cgaattttgg
     1201 tgcgatctga tgggaagacg aaggaaaaat cctgaacacg aaaaattacc tccaaatgta
     1261 tacccaaata aatatagtta tgtatggaaa ccaacatcca gagaatctgt aacactaacc
     1321 gccatcaagg atggtttagc tgctttatgg aaaaagtatg aggaaactgt aaataatcgc
     1381 gatcgtgcaa tgacattcgg tcgcttgtgg gaaaaattcc tcgccagcgc ctattacagt
     1441 gacctcagtc caagaacaca aaaagattat ctgcaacatc aaaaaaagtt gcttgccgta
     1501 ttcggtaagg tgccggcaga ttccataaaa ccagaacaca tccgtcgata catggacaag
     1561 agaggggaac agagtaaaac gcaagccaac catgaaaaaa gcagtatgtc ccgcgtttac
     1621 agttgggggt atgagcgagg gtacgtgaag gctaacccat gtgcaggtgt aagtaaattc
     1681 aaggccaaaa accgcgaacg atatgtaacc gacaaagaat accaggcagt attaagcgtt
     1741 gcacctcttc ctgtttttat cgcaatggaa attgcctatc tgtgtgcagc gagggtttcc
     1801 gatgtgttat cgctgaaatg ggagcagatt ggaaacgacg ggatctttat ccagcaaggg
     1861 aaaacaggaa aaaaacagat aaaagcatgg agtccacgat tacaggcggc gatcgaaaaa
     1921 gcaaaacagt taccaacatc cgcctatgta atcagcaatc aatacggcaa ccgatatatg
     1981 tacaaaggct ttaacgaaat gtgggtagaa gcaagaaatc gcgcaggcaa aatttcaggt
     2041 attttaaccg acttcacctt tcatgatctg aaggcgaaag gaatttcaga ctatgaagga
     2101 agcagtcggg ataagcaact tttctctggt cacaaaaccg aggggcaagt gctaatctat
     2161 gacaggaagg ttaaagtttc accgacactt gatgtcccgt tacctgaaaa tattccaaga
     2221 aaatattcca agtaattcca agtgtgattt ttgtcactga cttaatgatg tataagtgat
     2281 tgaattttgg cggagagagg gggatttgaa cccccggtgg agttgccccc actccggttt
     2341 tcgagaccgg tccgttcagc cgctccggca tctctccgtt cagatggttg ccatgatgcc
     2401 aggaaatttg gcattttaac agtccctgtc cgtgcaattt tgttcaagtg acgagtttgc
     2461 gagcaaaacg atgattaagt ggccctggaa agtacaagaa tcagcacatc aaactgccct
     2521 tccctggcag gaagcactat cgatccccct tttaacgtgt ctgacagaac aggaacaaag
     2581 caaattggtc gctcttgccg aacgtttttt acagcaaaaa cggcttgttc ctttacaggg
     2641 ctttgagctg aattcattaa gaagctgccg gatagcactt ctattttgcc tgcccgttct
     2701 ggagttagga ctggaatggc tggatggttt tcatgaagtc ttaatttatc ctgcgccatt
     2761 tgtggtcgat gatgaatggg aagacgatat cggtctggtg cataaccaac gtattgttca
     2821 gtcaggtcag agctggcagc aagggcctat cgttttgaac tggttggata tacaagattc
     2881 ttttgatgct tctggtttta acctgattat tcatgaagtc gctcataagc tggacacccg
     2941 taacggcgat cgcgccagcg gagttccctt tatttcgttg cgtgaggttg ctggctggga
     3001 acacgatctt catgctgcaa tgaacaacat tcaggaagaa atcgaattag ttggtgagaa
     3061 tgcggcgagc attgatgctt atgctgccag tgatcctgct gaatgttttg ccgtactttc
     3121 tgaatatttc tttagcgccc cagaactttt tgctcctcgt ttcccttcat tgtggcaacg
     3181 tttctgtcaa ttttatcaac aagatccttt gcagagactg catcacgcta atgatacaga
     3241 ctcgttttca gcgacgaatg ttcattaatt aacaactttg aagattaatt aaccaattga
     3301 aatggcttat gaaatttagt gttgacagac aaggtaccgc taagtaatat gcgccccgtt
     3361 c
//
LOCUS       DAJTSI010000258         3324 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000258 DAJTSI010000000
VERSION     DAJTSI010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.263"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            454..1281
                     /locus_tag="OO327_004021"
     CDS             454..1281
                     /locus_tag="OO327_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR6020353.1"
                     /translation="MKKVIPALALVLLTTGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            1325..2074
                     /locus_tag="OO327_004022"
     CDS             1325..2074
                     /locus_tag="OO327_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR6020354.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            2071..2640
                     /locus_tag="OO327_004023"
     CDS             2071..2640
                     /locus_tag="OO327_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020355.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            2764..3006
                     /locus_tag="OO327_004024"
     CDS             2764..3006
                     /locus_tag="OO327_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR6020356.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLFEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            3010..3156
                     /locus_tag="OO327_004025"
     CDS             3010..3156
                     /locus_tag="OO327_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020357.1"
                     /translation="MNKAFELWVHQRYGNHYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LSVV"
     gene            3165..>3324
                     /gene="xisR"
                     /locus_tag="OO327_004026"
     CDS             3165..>3324
                     /gene="xisR"
                     /locus_tag="OO327_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HCR6020358.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     HVSPDGNPK"
BASE COUNT          928 a          796 c          854 g          746 t
ORIGIN      
        1 acgccatcct gacaagcgtt ttatcttcaa cgggaatgag atatggaaac gtggagaatc
       61 aaccacagag tatacccgcg cacaccagcg aacaatcaga cgactgcgca gactcatcgc
      121 caggaaacag tatacagaaa aatggttcag aaaatatgac acatggagcg ccggatatta
      181 cgaactgatg gcaacaaaac cattctgacg taaacgaaat taaccatgac gcaattaaat
      241 aaggcaagcc gaatacatca ggaggaccat gaacatttat ttcagaatag ttatatcact
      301 ggcaattatc gcatgtattt acggattaat cgttccggcc ctcatatcaa tgaaggatac
      361 ggtagcagtg atttctggct ttgctctggc gtgtctgacc ccgccctgca tttatgccat
      421 ttataagggt ctttcttttt ctaaggataa aagatgaaaa aagttattcc tgctttagcc
      481 cttgttctgc tgaccaccgg acttgtcggt tgcgatcgcg ttgagcctgg taatgttggc
      541 atcaaggtaa acaaactggg cgacgataaa ggcgtcggag aagtggtcgg tgttgggcgc
      601 tactggacgg gatggaacac tgaggtttac atcttcccga ccttcaaaca aatgaagacc
      661 tacgatgagc cattcagctt ccagatgagt gacggtacaa ccatcggcta tcacatcggc
      721 gtggcctaca aagttgatcc atccaaagtt accacagtct ttcagaccta ccgcaaaggc
      781 gtggacgaca ttaccgacac cgacctgcgc cagaagatag ccgacgcact caaccgactg
      841 gccagcaaaa tgaccaccga caaatttatc gacggcggca agtctgagct gctggatgca
      901 gctcttaaag acattcagga agaaatgacg cccatcggta ttcaggtaat gagcctctca
      961 tatgtgggta agccggagta cccgcctact gttatcgaca gcattaatgc caaagtcacg
     1021 gcgaaccaga aaaccctgca atgcgagcag gaagtaaaac agcgcgaagc ggaagccaac
     1081 atgttgcgcg cggaagctgc cggacaggca gatgcgattc gcacaaaagc ccaggccgaa
     1141 gccgatgcta ttcgtttacg cggtgaagct ctgcgccaga acccaaacgt tatggagctg
     1201 gaagccatca acaagtggaa cggtacactg ccgcaataca tgaccagcgg tgccaacaca
     1261 ccatttattc aggttaagta atacatatgc ccggtattac acgccgggct gtctggagat
     1321 aaaaatgaat actgtaacca tcaacaacaa acagtttccg gtaatcgaat atcgcggtca
     1381 gcgtgttgtg acgctggcga tgattgatga agtccaccag cgaccggaag gaaccgcgcg
     1441 tgcagcattc aaccgaaacc gtgagcattt catcagcggt gtggattatg ccgaattagg
     1501 tgcggacgta atacgtacgg acctcccgga aggaacattc tctaaatttg caccatcggg
     1561 gattgtactt tttgaatcag gttacctgat gttgacgaag ccattcaatg acgcccttgc
     1621 ctggcaggtt cagcgcgaac tggttaacag ctacttccgc actcgcacac cgctaacgga
     1681 aatcgagatg atcgctgcga tagccgccga cgctgttcgt caacagaagc gcctgaatca
     1741 tgtggaagaa cagctcgaaa cagtcacaga agcggtggaa accatcaaac gcggaaatat
     1801 gcgcgccgga tatgtcggtt accgccaggt ggtagccaaa agtggaatga gtgacaccaa
     1861 gtgtcggaat ctggtgaacg catatcaaat tcctacagac acccacgaat tcatgactcc
     1921 ggacggtctg ttgtctcgca gggctatcgt cgagcttgag ccatttatgg cggcgttcca
     1981 ccaaatgatg tcagaggctg acccacgcgg cacacgctgg tatcacccga agatggggct
     2041 tttccaggtg atcggatggg aggataaagc atgatcatcc agtcaaaact tattcgcgcc
     2101 gctctggtgt gcgctgctaa aaacgacgtt cgttattacc tgaacggtct tcacatcacg
     2161 ccgaaacata ttgaggcaac caatggttcc gtggcactgc gcatggctca cggcatccgg
     2221 acgaagaaaa acatcattgt ccagttcgaa gggggcgtcc cggccaaagc cgaaacgaca
     2281 gagctgattt ttagtaaaga gccgatcgct gttcatcgcg accagtttca gcgacgactg
     2341 tccattaccg gcattaaact ggtggacggt tgttttccgg atttggatcg catcattccg
     2401 aaaaagtttg accgctgtac tcacccggtg ctccaggcgg gttacctgag ttatccagag
     2461 aagatgtttg gtcgtgagcg taaatttatt cccgtccagt tacgtccctc cggtgacggg
     2521 caagcggtca gaattcagtt tgattccatc atcaactcaa tgtatggcaa tcctgaattt
     2581 gttgtgatgc cttgtcgtga tcatggcgat ttcaacgtgg ctcaggagca tccggaatga
     2641 aaatcgaata ccaggacgcc gcaggaggtg aatcaatgag ctggcctgat gcaatcgtaa
     2701 ctctggggat ggtattcgca gtagcgtttg ttgtgtactc gatttgtcga tggggataac
     2761 cacatgttcg ctttgattca acgcgggcag atatacacag acagagccgg atacccagta
     2821 gaaattactc gcagtactga gcactcagtg ttctttcgga ggatggacgg acgtaccggg
     2881 cgagtacgca tcggtgaatt cagtagcctg ttcgagcata ttgaccacct ggaatatcac
     2941 aagattctgg ctgaaacaga acaagaaaag cacctgaaga aattacgcgc aatgcaaagg
     3001 aagtaaaaaa tgaataaagc gtttgagcta tgggtccacc agcgttacgg caaccactac
     3061 gacctgacgc gagatgttga cggtttctac tgtcgtgaaa ttgtgaaacg aatgtttgag
     3121 gtctggtgcc actgccgtgg gctgagtgtt gtgtgaggta atgcatgggc aatgtgattc
     3181 aactggctcc caatgaatgg gtttgtgaaa gcgttctaat cgcaattacc gggctcaaac
     3241 caggcacaat tcttcggacc cggaaagaat gctggatggt tggaagagag tatattcacg
     3301 tatcaccaga cggtaatcca aagc
//
LOCUS       DAJTSI010000259         3313 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000259 DAJTSI010000000
VERSION     DAJTSI010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.264"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..98
                     /locus_tag="OO327_004027"
     CDS             <1..98
                     /locus_tag="OO327_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR6020359.1"
                     /translation="RLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            156..512
                     /locus_tag="OO327_004028"
     CDS             156..512
                     /locus_tag="OO327_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020360.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            605..823
                     /locus_tag="OO327_004029"
     CDS             605..823
                     /locus_tag="OO327_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR6020361.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            825..1190
                     /locus_tag="OO327_004030"
     CDS             825..1190
                     /locus_tag="OO327_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6020362.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEIYQEGATA"
     gene            1187..1852
                     /locus_tag="OO327_004031"
     CDS             1187..1852
                     /locus_tag="OO327_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020363.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1852..2217
                     /locus_tag="OO327_004032"
     CDS             1852..2217
                     /locus_tag="OO327_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR6020364.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2672..2827
                     /gene="hokD"
                     /locus_tag="OO327_004033"
     CDS             2672..2827
                     /gene="hokD"
                     /locus_tag="OO327_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR6020365.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
BASE COUNT          959 a          673 c          855 g          826 t
ORIGIN      
        1 ggcgcctgaa tataatctgt cgcgagtgcc ggaagagcga agctatgcag cgtgtactgg
       61 ctttctatca gggtaatttt caggaggcga tactgtgaat gaaattagct atcaggcttc
      121 aattaccgct ggcattcgca tcaaaggaga ggagcatgga aataaaacca gaagatgagt
      181 taagcaatat cgttttattt ccgataaaag aggatgaccc tcgtaatcag gttaattttc
      241 tttataagcc atcggaaaga ccatattgcc atcacgcctc tgtacgggtt gacgaaaaag
      301 agcgtcaggt ccgctgtaaa atctgcggtg cggttgtgga gccgtttgac tggatgctct
      361 ctgtggcaaa aagagaaacc agactggcag atgatgtaag gctattgcgc caggaggaac
      421 aggaaaggcg gagaaatata gaaaagctga tacagattga gcgtaacgcg aaagcgcgga
      481 tacgcagggc gacaaaatcc agaactgaat aattaaattt agcactgtta aaaatttaat
      541 ccttaaccgg agggatttct gcaccctcaa atcatcagga gaccgcccga aagggcggta
      601 atgaatggtt acattattta gaaaaaaata tccgcgaaag agtagaacaa cagaattcct
      661 gtttctcatt ctgtttatcg tgttgatgac accgatatcc ccgcaaattt ttgtctggat
      721 aatcggaaaa ataattgagc cagttattga attgtataac gacgtggtat gggcgtcatt
      781 caacacactg cacaataaaa ttaatccgta taaggaaagc tgatatggca ctgacgaaaa
      841 aacaacgtgc agaactgcgc atgaagttcg gtggtcgctg cgcttattgc ggctgcgaac
      901 ttggcgaaaa gtggcatgca gaccatgtaa aaccggtcat tcgttttgat ggaaatatgc
      961 ttcaccagga acgtgacgat atatccaaca tggtaccaga atgccaccca tgcaatctgc
     1021 acaagcattg cagtagcctg gaagattatc gacgaattat cagtgatggt cgtcgtgaat
     1081 tccttgcgtc cgggaaaggc aaagcgctgg ttcgtatggg attggttgaa atgaaatctg
     1141 acccggttgt gttctggttt gaaatatatc aagaaggggc tacggcatga ctactattac
     1201 caaagagcga ctgcaatggc tggctaacat ttctggccgc gatgatattg acgatataga
     1261 cggcggtgaa attcgtgaac tggcgcttat cgctctggca tcactggaag cagagcctgt
     1321 agcggaatgc attgttgaag atgggggcat gtgtattgat gggttcggtg agtatgtggg
     1381 tcactcgctg cctgatggaa tgcatgagct ttatgctgcc ccgcgaatgc gacaacctga
     1441 tggatggaaa gccgtagctg tagcgtggaa ggtgacgttt actcaagttg accaggaatc
     1501 taatacgttc actgctatat atttttacaa agcggaagtt gagcgttggg tacgactgca
     1561 taaagcatgt gattttcggg cagatataac accgctttac gcagcgccgg cagtgccggt
     1621 tgcagtaaac gacgacatgg cttacgcatt ccatcacgca ctgtcagatt catcgcttgg
     1681 ctctgatgaa atcgaagaaa ttaaaaccgg tttgcgtgct gcctttgcca acgtcactat
     1741 ccaaccagag ccgatagtgc cggatgaaat cgggccaaac gatagtaata cgtttgatta
     1801 tgttgatggc tggaacgcct gccgcgctgc catgcttaag ggagataaat aatgattaat
     1861 cgaaccaaac tggagcacat tctcgaatat gccaggcagc agaaatgtat tgggcaactt
     1921 tgtaaaattc caccaggaga tatggttgaa atcgtggaaa tggccatgcg taaggctggc
     1981 aactctccgg taactctggc tggctggata agctgtagtg atgcagttcc tgctgaatat
     2041 tgcgatgtga ttcttctcga tgatctcggg aatgtattcc ccggttcctg ggataaggtt
     2101 ttttgcccca ctcgtggcgg gaataagatg gcttttgtgg acaaagacgg cgttgaagta
     2161 gagagctcaa ctcactggat gccgctaccg gaaccaccgc aggaggtgaa tcaatgacct
     2221 ggcctgaggc attcaccacg gtaggaattg cgatggcggt ggcgctggtg gtgtattcga
     2281 tttgccgttg gggttaacaa acaaaaaccc cggattgatg gtccggggtt tttgaaggaa
     2341 acaaaacaga aacagcaatt gccgttacct gttgttacca tggcaagtaa acgtatctca
     2401 ggcgagcgca ttgcgccgtt ctgacgcaga taaattagcc tggatagaag gtgctggcaa
     2461 taaaaaatag cgttttctta tcggtatcgg taagattgct gcgggtgctt gaggctgtct
     2521 gcctcgggca tgccactgta aggcagacag agaaaagccc cagttaacat tacgcgtcct
     2581 gcaagacgct taacattaat ctgaggccca atctatgtct cacaaatgta ggttagcctc
     2641 ttacgtgccg aaaggcaagg agaagcaggc tatgaagcag caaaaggcga tgttaatcgc
     2701 cctgatcgtc atctgtttaa ccgtcatagt gacggcactg gtaacgagga aagacctctg
     2761 cgaggtacga atccgaaccg gccagacgga ggtcgctgtc ttcacagctt acgaacctga
     2821 ggagtaagag accaggcggg ggagaaatcc ctcgccacct ctcatgtgtc aggcatcctc
     2881 aacgcacccg cactaaaccc gcttcggcgg gttttttgtt gcgtgctgaa tacgcagggt
     2941 gaaaaataac catatatttg attatataca caacaaaaaa taaaagtcat tgtacttgca
     3001 cattaaacaa tcaaatatac ggcgtgaaat aaatatttat cagattaata tttttgtctc
     3061 tatgtggata taaccgtttg tacttataaa cctgggggca tcgtggaaaa aataaagaaa
     3121 ctatttagta gcaaatacgc agtcatacgt cgtgatgacc tgtcagttat agtcgaaatg
     3181 gattacttcc ctgaaacccc aaaatcaata atgtatcgta atggtcgaaa ggcaattttt
     3241 ttaccgatga gggtaagtga cattatggga aatgataaac tgctggatga attgcgagtc
     3301 agagcatcct gtt
//
LOCUS       DAJTSI010000260         3258 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000260 DAJTSI010000000
VERSION     DAJTSI010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.265"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..442
                     /locus_tag="OO327_004034"
                     /pseudo
     CDS             1..442
                     /locus_tag="OO327_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1026..1742
                     /locus_tag="OO327_004035"
     CDS             1026..1742
                     /locus_tag="OO327_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020366.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSFIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWFFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2294..2698
                     /locus_tag="OO327_004036"
     CDS             2294..2698
                     /locus_tag="OO327_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020367.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2761..3258
                     /locus_tag="OO327_004037"
                     /pseudo
     CDS             2761..3258
                     /locus_tag="OO327_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          909 a          675 c          749 g          925 t
ORIGIN      
        1 gcgttctaag ccttcccacc atgatcgggg aagccccaga tatgaacagg cacattcaga
       61 gttactttaa agagcttact gaacgtctga ggtgttagag ccatgactgc cgcagcattg
      121 ctggcggaaa tgcctgaatc gagcagcctc tcgagacgtg aaatcagcgc acttgtcggt
      181 gttgctcagg taaaccggga ctcgggaact ctgcgcggtc gacgaaccat tttcggtgat
      241 tgtgctgggg aagaacagct ctgtacatgg cggcgcttga ggcccccccc ggtttaatct
      301 ggtgataaag gcgttttaca tgcgcctact tgcagccgga aacgccaaaa aagtggcact
      361 ggttgcctgt atgcgtaagt tgctgaccat tatgaacgca atgctcagaa aaaacgaaga
      421 atggaatgaa tcgtatttat gaaaactgga ttgtgggcca taaatattcc aaaaagatag
      481 cccgctccgt ttattaccgg aacgggcgag agcatgacat tgtctgttat tataatgaga
      541 ctgctgattc tacttatttt taatttcaag aaaagcgttt tctacaaaga attatgagat
      601 ttaatgtttt gtttagacaa tattatcatt ctgttcaata atgaaagtct tcatcaacaa
      661 caagtaaaaa caacgtccct ctcatcgaac cggacttgca gatttcccgc atccggctct
      721 cttgcaacac atcagacctt cacgttcggg cggttatggg gaaggtccag caagctgtat
      781 gagccctaac tgtttgaata ccagtagatt accagaaact tctatcgatt gaagacatta
      841 ccaactagcg ttactaaatg ttgaccaagt tttgttcact tgtctggttt tgatagtagc
      901 attctgcaga gtattttgat atgataaata tgcattaaca atgatattat tgttaatttc
      961 ctagcatgaa aattaaaaga tcaactttta tctcgaatat tttttacatt atcagttagt
     1021 ttcttatgaa tgacaattca ttgcttagaa attcttcatt attcatcgcc tatatgggat
     1081 gtgtagggtg ggtcagtgct tattcttatg gctggggaac ctctttttac tatggatttc
     1141 catggtgggt tgttggtgca ggtttggatg atgttgccag aagtttactc tatgctatca
     1201 tagtaatggg tatacttttt accggttggg ggattggtat tttatttttt cttcttataa
     1261 agaaaagaag taaaatacaa gacttgagtt tttttagact attttttgct attaccctac
     1321 tgtttttccc tgtaatattc gaacttttaa ttctcaaaca atatttcata cttccattat
     1381 cactttcatt cattattagt tcattggtta tttcaataat aattcgtata tatggacgga
     1441 tcttttctgt atcatgtttt tcagatatcc cttttgttag agaacaccgc attaagttaa
     1501 ttatggctgg ttttttggtt tatttttggt ttttttcctt tcttgttggc tggtataaac
     1561 ctcaattaaa aaaagaatat cagatgcttt gttataataa tagttggtat tatgttcttg
     1621 ctcgttatga ttcacggctc gttttatcta gttcatttaa agatgacagt aacagattct
     1681 taatatttaa tacagaacag tcgggatttt atgaaatcaa tgatgtttat gtcagaaagt
     1741 aataatcctc tcccacaaag tgaattaaat gaatatataa tgcgttcgaa gtatatgtat
     1801 tgagctgtca tactgagaaa caaaccttag ggcagcacaa ttaaagccag aaacaactat
     1861 gcaagaaagt tactaaataa gtgctcggaa cagtaaaaca ctcagggggg actcagtccg
     1921 gattatgtaa tcagataaat tgccaaacat cacaaatcac cagccggact gagtgatgcc
     1981 gagcattgca ccaatacccc gtgatgaacg atgcctgatg cagaaagctc cctataaaac
     2041 aaagaacaaa aactacgacc ggcgattcac cgtcatactg atgctgcatc gtggtgacac
     2101 tgtctgtcac tttcatgttg actgaacaac tctgctgcga agaaggtgta ccgccggcgg
     2161 gatttgattg tccggcgtct agcttccact tccctgatgg aactgaaagt tgttatggcc
     2221 ggtcagcttg ccctgtccgc aggacatgag gtgctgtgac ggacagcacc agtaacggaa
     2281 agtgacaatg aaaatgaata ctgcgctgat agttgcacta atgtgtatgt ggtatgcggt
     2341 acccgctgct gcaaaagaga ctttactggc tatgccacgt aattcaacag aacattgcta
     2401 tgcggagatt aatgtgcacg gaccatatgg ggtgtatttc cgagtggtcc cgcatccccc
     2461 cgggggaaag agctgggtcg aatgtaacag tgactactat tactctgata aaccaccggg
     2521 agtacagata ctcggaaccc gtgcagggtg tcgggtatac ggaatctgcg ggaccacctc
     2581 gacactgcat gtggctggaa gaggggttgt gtgtattaaa aatatatgca gtccacgtgg
     2641 gatgataatt cacaggatac ggaaacgtcc tgtagtggct gtaagcgatg agatgtgatc
     2701 ttacccagca atagtagaca cgcggctaag tgagtaaact ctcagtcaga ggtgactcac
     2761 atgacaaaaa cagtatcaac cagcaaaaaa acacgtaaac agcattcgcc tgaattttgc
     2821 agtgaagccc tgaagcttgc tgaacgcatc ggtgttgctg ccgcagcccg tgaactcagc
     2881 ctgtatgaat ctcagctcta tgcgtggcgc agcaaactgc agcaacagat gacctcgtct
     2941 gagcgtgaaa gtgaactgcc cgcctgaagc ggcaactggc ggagcaggca gaggaactgg
     3001 ctatcctcca aaaggccgcg acatactttg cgcagcggct ggtatacgtg gtgtcagcgt
     3061 cggacaggaa taagccctcg tcagcagttc cgtcagcact gcgacagcgt tgtcctcgcg
     3121 gcggccttta cccggtcaaa acagcgttac ggtgcaccac gcctgacgga tgaactgcgt
     3181 gctcagggtt accactttaa cgtaaaaacc gtggcggcaa gcctgcgccg tcaggggctg
     3241 agggcaaagg cctcccgg
//
LOCUS       DAJTSI010000261         3195 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000261 DAJTSI010000000
VERSION     DAJTSI010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.266"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(36..1391)
                     /locus_tag="OO327_004038"
     CDS             complement(36..1391)
                     /locus_tag="OO327_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR6020368.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
     gene            complement(1395..1679)
                     /gene="gatB"
                     /locus_tag="OO327_004039"
     CDS             complement(1395..1679)
                     /gene="gatB"
                     /locus_tag="OO327_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR6020369.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(1710..2162)
                     /gene="gatA"
                     /locus_tag="OO327_004040"
     CDS             complement(1710..2162)
                     /gene="gatA"
                     /locus_tag="OO327_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR6020370.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(2172..>3195)
                     /gene="gatZ"
                     /locus_tag="OO327_004041"
     CDS             complement(2172..>3195)
                     /gene="gatZ"
                     /locus_tag="OO327_004041"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCR6020371.1"
                     /translation="RIILGGDHLGPNCWQQENADAAMEKSVELVKAYVRAGFSKIHLD
                     ASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQREQLSYVIGTEVPVPGGEASAI
                     QSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIAIVVQPGVEFDHSNIIHYQPQEA
                     QALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFAILKVGPALTFALREAIFALAQI
                     EQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGFNDSLLDIRYSLSDRIRYYWPHS
                     RIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQSGELSAMPHQLIMNKIYDVLRAY
                     RYGCAE"
BASE COUNT          887 a          847 c          693 g          768 t
ORIGIN      
        1 ttgttaaggg ggataacctc cccctcaggt aaaaattatt ctgcgagaac ggctttctct
       61 tgtttagtaa aaccacgcgc tctacgccag gtcatgaaaa taccggtcag ataaattgcg
      121 ccgataataa tgaaaccggg aatattttgc ggggagaaaa cctgaatcag taaccaggta
      181 atgggagaac cgccctgatc cattgaagcc accatacccc cggcttttaa cgccccagca
      241 ttagccgccg gttgggtgtg caaaccaata gtttgcgtcg cgatccacag ggtgatgctc
      301 ataatgatga cacccgagat taaggtgcgg aacagatttc cacgatgcac cgcgaccgcc
      361 atcgccacga agaagccgat agtggcaaga tcgccaaacg gcagtacctg attacccgga
      421 acacaaacag caattaaaat ggtgagcgga ataaaaatca ggcttgccga taccaccgcc
      481 gtatgcccca gcagtaatgc gggatccagg ccaatcagga actcctgacc gccgaacttc
      541 gcctgtaaac gactacgggc ctgcttagcg atgggcgtta aaccatccat gatgggttta
      601 atcatccgtg gcatcagcag cataactgcc gccgttttta ccgccagctg caatacacct
      661 ttgacatcgt aacccgcgag gatgccgata atcagcccca tcacaaaacc cacggtgaca
      721 ggttcgccaa aagggccaaa tttgcgctga atatcgtcgg cgctaaattt aattcggtta
      781 acgcctggga ttttctcgat gatagcatcg accagcaccg caatcggccc catatacgcc
      841 gacgtaccgt gcggaatagc aataccttcc agctcaaaga aatttcgggt atcgcgggca
      901 aaccagtcgc cgagcttata aacaaacgcc gcgtgaatta ccacacccgc catccctatc
      961 atccatgaac cggttgccag gtgcagcaac gcgccggtga aggtcatatg ccagatattc
     1021 cagatatcaa catttaccac ccgcgtcata cgggtcagta acatcgccac gttaaccaat
     1081 atcgcaatcg gaatcgccac cagcgcaatt tgcgacgccc aggtcattgg tgaagagccc
     1141 ggccagccga catcgaccac atgcagattc aggtcgaaat tttccgccat cgctttcgcc
     1201 gccggaccaa tggaatccag cattaagcca atcacaaggc caatgccaac aaagccaatc
     1261 ccgatatgca gacccgcttt aaagcaatcg cctgccttca tgcctaatat tttagaaaaa
     1321 ataatgatga caaccggcag catcactgta gggccgaggt cgagaatata acgcatgact
     1381 tctgaaaaca taggtcatcc ctgtaagata gtcagaattt tattttgtaa tgcttcgata
     1441 ccgacaccag aaacaaaagg catgccgtga actaacggaa tatcgccaaa actacgatcc
     1501 accctggcag tggtacatat caaatgcaca ccatccatat aggtttctat ttcattaacc
     1561 cgacactgga ttaattcaac aggaatatta tgactctgac acaactcttt aatttcttcc
     1621 gccgccatcg tagaggtcgc aaccgcgcct ccgcaagcga caataatctt gcgtttcata
     1681 aatagtcctt tttattgtta cagagggatt tattcgtctg aatctaaaac atactttgtg
     1741 aagtattcct ttaactgagt ttcaggaaga gtaattagtg tctcgacgat atcgggctgt
     1801 tgtaacttgc caaataaaca gcgtaaaagt ttcaattgct gctgcggatt ttccacaatc
     1861 aacgcaataa ccaacgatac cgccacgtcg ttatcatcat ccgcttgctg aaaatgaact
     1921 ttatttgttg gccttaacag ataaatggct gacgacttag catgaatcgc ctcacaatgc
     1981 ggtattgcaa tggcgtgctg ctcaagcatt atcccggtag ggaattctgc ttctctggca
     2041 attaatgcct gtggccaggt atcatgaacc acacctttcg cgagcatctc attaccgata
     2101 taggttaaaa cttcgctacg atcgacaaaa gaaattccgc tacgaacaaa caggttagtc
     2161 atataccgtc cttattccgc acagccgtag cgataggcgc gcaaaacatc ataaatttta
     2221 ttcataataa gctgatgcgg cattgctgat aattccccgg actgaatgcg ttcaaattgt
     2281 ttgggaagat actgactaat catgcccagt gggatgtcca cgccttgcag attcaccatc
     2341 atcgtttcaa cgctattttt aatccgacta tgcggccagt aataacgaat acgatccgac
     2401 aggctgtaac gaatatccag taatgaatcg ttaaaacccg tgcgataata ttttttccag
     2461 tattgcggtt cgtcgagcat cacttcttca attaccgcca ggcaaccgct gcgattttcg
     2521 ggggcgataa gttcctgctc aatttgtgcc agtgcaaata tcgcctcgcg taaagcaaag
     2581 gttaatgcgg gaccgacttt caatattgca aagtgatcgc ggactaattc ccaataagcc
     2641 gtccgggtct ggtaatcggt agaatgtgct tcataaacca ttcgggtgtt ttctatccat
     2701 tgcgccagtg cctgcgcttc ctgcggctga taatggataa tattgctgtg atcaaattcc
     2761 acacccggct gcaccacgat ggcaatcaca cgtgttaacg cctctgtcag cccacgggca
     2821 ataaaggcct tttgatgcgt acgtaaagta ttggcggcat cttcaacatg ggtgatgtgt
     2881 actgactgaa tggcgctggc ctcaccgccc ggaaccggaa cttcagtgcc aatgacatag
     2941 ctcagttgct cacgctggca atctgtcgcc acactttccg cagcaaagca aagcacagca
     3001 gctcgttccg caaccgtttc tggtgctaac ggtatggtat cccccgcgca ggacattgac
     3061 gcatcaagat gaattttact gaagccagca cgaacatatg cttttaccag ctcgacggat
     3121 ttttccatcg ccgcatccgc attttcttgc tgccagcagt ttggtcccag atgatcgccg
     3181 ccgagaataa tgcgt
//
LOCUS       DAJTSI010000262         3177 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000262 DAJTSI010000000
VERSION     DAJTSI010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.267"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            168..1271
                     /gene="proB"
                     /locus_tag="OO327_004042"
     CDS             168..1271
                     /gene="proB"
                     /locus_tag="OO327_004042"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR6020372.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1283..2536
                     /gene="proA"
                     /locus_tag="OO327_004043"
     CDS             1283..2536
                     /gene="proA"
                     /locus_tag="OO327_004043"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR6020373.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2651..2726
                     /locus_tag="OO327_004044"
     tRNA            2651..2726
                     /locus_tag="OO327_004044"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2684..2686,aa:Thr,seq:cgt)
     gene            complement(2741..>3177)
                     /locus_tag="OO327_004045"
     CDS             complement(2741..>3177)
                     /locus_tag="OO327_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6020374.1"
                     /translation="NRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGTKAPT
                     VRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWESIEM
                     VRRYAHLAPNHLTEHARQIDLILNPSVPNLSQSRNKEGTNDV"
BASE COUNT          750 a          804 c          877 g          746 t
ORIGIN      
        1 tggtaattta ttaaatctgt aataaaagcg taaacaactg ccgctacgct tgctgatccc
       61 gcgcaacaaa acgccatgct ttgctcgcag atggttggca accgacgaca gtcctgctaa
      121 aacgttcgtt tgatatcatt tttcctaaaa ttgaatggca gagaatcatg agtgacagcc
      181 agacgctggt ggtaaaactc ggcaccagtg tgctaacagg cggatcgcgc cgtctgaacc
      241 gtgcccatat cgttgaactt gttcgccagt gcgcgcagtt acatgccgcc gggcatcgga
      301 ttgttattgt gacgtcgggc gcgatcgccg ccggacgtga gcacctgggt tacccggaac
      361 tgccagcgac tatcgcctcg aaacaactgc tggcggcggt agggcagagt cgactgattc
      421 aactgtggga acagctgttt tcgatttatg gcattcacgt cgggcaaatg ctgctgaccc
      481 gtgctgatat ggaagaccgt gaacgcttcc tgaacgcccg cgacaccctg cgtgcgttgc
      541 tcgataacaa tatcgttccg gtaatcaatg agaacgatgc tgtcgctacg gcagagatta
      601 aggtcggcga taacgataac ctttctgcgc tggcggcgat tctggcgggt gccgataaac
      661 tgttgctgct gaccgatcaa aaaggtttgt ataccgctga tccgcgcaga aatccgcagg
      721 cagaactgat taaagatgtt tacggcattg atgacgcact gcgcgcgatt gccggtgaca
      781 gcgtttcagg cctcggaact ggcggcatga gtactaaatt gcaggccgct gacgtggctt
      841 gccgtgcggg tatcgacacc attattgccg cgggcagcaa gccgggcgtt attggtgatg
      901 tgatggaagg catttccgtc ggtacgctgt tccatgccca ggcgactccg cttgaaaacc
      961 gtaaacgctg gattttcggt gcgccgccgg cgggtgaaat cacggtagat gaaggggcaa
     1021 ctgccgccat tctggaacgc ggcagctccc tgttgccgaa aggcattaaa agcgtgactg
     1081 gcaatttctc gcgtggtgaa gtcatccgca tttgcaacct cgaaggtcgc gatatcgccc
     1141 acggtgtcag tcgttacaac agcgatgcat tacgccgtat tgccggacac cactcgcaag
     1201 aaattgatgc aatactggga tatgaatacg gcccggttgc cgttcaccgt gatgacatga
     1261 ttacccgtta aggagctggc cgatgctgga acaaatgggc attgccgcga agcaagcctc
     1321 gtataaatta gcgcaactct ccagccgcga aaaaaatcgc gtgctggaaa aaatcgccga
     1381 tgaactggaa gcacaaagcg aaatcatcct caacgctaac gcccaggatg ttgctgacgc
     1441 gcgagccaat ggccttagcg aagcgatgct tgaccgtctg gcactgacgc ccgcacggct
     1501 gaaaggcatt gccgacgatg tacgtcaggt gtgcaacctc gccgatccgg tggggcaggt
     1561 aatcgatggc ggcgtactgg acagcggcct gcgtcttgag cgtcgtcgcg taccgctggg
     1621 ggttattggc gtgatttatg aagcgcgccc gaacgtgacg gttgatgtcg cttcgctgtg
     1681 cctgaaaacc ggtaatgcgg tgatcctgcg tggtggtaaa gaaacctgtc gcactaacgc
     1741 ggcaacggtg gcggtgattc aggacgccct gaaatcctgt ggcttaccgg cgggtgccgt
     1801 gcaggcgatt gataatcctg accgtgcgct ggtcagtgaa atgctgcgta tggataaata
     1861 catcgacatg ctgatcccgc gtggtggcgc tggtttgcat aaactgtgcc gtgaacagtc
     1921 gacaatcccg gtgatcacag gtggtatagg cgtatgccat atttacgttg atgaaagtgc
     1981 agagatcgct gaagcattaa aagtgatcgt caacgcgaaa actcagcgtc cgagcacatg
     2041 taatacggtt gaaacgttgc tggtgaataa aaacatcgcc gatagcttcc tgcccgcatt
     2101 aagcaaacaa atggcggaaa gcggcgtgac attacacgca gatgcagctg cgctgacaca
     2161 gttgcaggca ggccctgcga aggtggtggc ggttaaagcc gaagagtatg acgatgagtt
     2221 tctgtcatta gatttgaacg tcaaaatcgt cagcgatctt gacgatgcca tcgcccatat
     2281 tcgtgaacac ggcacacaac actccgatgc gatcctgacc cgcgatatgc gcaacgccca
     2341 gcgttttgtt aacgaagtgg attcgtccgc tgtttacgtt aacgcctcta cgcgttttac
     2401 cgacggcggc cagtttggtc tgggtgcgga agtggcggta agcacacaaa aactccacgc
     2461 gcgtggccca atggggctgg aagcactgac cacttacaag tggatcggca ttggtgatta
     2521 caccattcgt gcgtaaataa aaccgggtga tgcaaaagta gccatttgat tcacaaggcc
     2581 attgacgcat cgcccggtta gttttaacct tgtccaccgt gattcacgtt cgtgaacatg
     2641 tcctttcagg gccgatatag ctcagttggt agagcagcgc attcgtaatg cgaaggtcgt
     2701 aggttcgact cctattatcg gcaccattta aatcaataag ttacacatca ttagtacctt
     2761 ccttatttct tgactgggac aaatttggga ccgatgggtt cagaatcaag tctatttgcc
     2821 gtgcgtgttc ggtaaggtga ttaggtgcga ggtgagcata tcgacgaacc atttcgatag
     2881 actcccagcc tcccatttcc tgtaacactg ataacgggac tccggcttga accagccaac
     2941 ttgcccaggt gtgtctcaag tcgtgaaatc tgaaatcatc aataccagcc cgtctcagcg
     3001 ccgctttcca ggctgtgttt gcgtcatacc gcatcttcct tactgttggc gctttcgttc
     3061 cgtctggttt ggtacagctt tccttgtaca caaataccca acggtgatga tttccgattt
     3121 gttttttcaa tacgcgacat gcagtatcat tcagcgcaac gccaattgcg cggtttg
//
LOCUS       DAJTSI010000263         3149 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000263 DAJTSI010000000
VERSION     DAJTSI010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.268"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            801..1910
                     /gene="fepE"
                     /locus_tag="OO327_004046"
     CDS             801..1910
                     /gene="fepE"
                     /locus_tag="OO327_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR6020375.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2935..3129)
                     /locus_tag="OO327_004047"
                     /pseudo
     CDS             complement(2935..3129)
                     /locus_tag="OO327_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          798 a          652 c          759 g          940 t
ORIGIN      
        1 gtggtaattg taagacgttg cgggcgacgg tattacaatt gcacatcctg tcctgtttct
       61 ttaggacgta tcatggcaca gcatatcaac tgataaataa tccctgtgca gtgcagccgc
      121 tgttgcataa aatttcgctc tgcgtcgagt gtttcttaat caataattcc gctgatactc
      181 cggaaaaacg cgagtctgtt ctcatgcctg aaatgcccac accccacgca aaaacaagtt
      241 tttgctgatt tttctttata aatagagagt tatgacaaat tagttcttct tgctctcttt
      301 gtgatattta aaaaagcggt gtcggcgcgg tgttgtagct gcgccaacac cgctttttag
      361 gggtggtact gactattttc ataaaaaaaa acattatctt atattagggg tgctgctagc
      421 ggcgcggtgt gtttttttac aggacgcccc tgggggcgct gctaggggtg tctgttcaga
      481 tatgtagcaa tgagtcaggg attcacgaac tgaaaatgtc cgaaaatgtg tctcatgggc
      541 actgtatgcg cccaaaccga tcgtcattca ctttcatgca tagctatgca gtgagctgaa
      601 agcgatcctg acgcattttt ccggtttacc ccggggaaaa catctctttt tgcggtgttt
      661 gcggcagaat cgcgttcagc gcgttttagc ggtgcgcgca ataaaatgtt atgttaaatg
      721 tctgctgaat ggtttttagg ttttttccta acatatgata gaataaaaaa accatatgtt
      781 aatatattgt gtttttttca atgaaaatct caaaggtctc tgacgaacat ttttctaatt
      841 acaacatgtt gccgaatggt aatgaagaaa ttgacctttt tggcttgatt agcgttttat
      901 gggcagcaaa aataaaaatt gttggggtga ttctattttt tgcttgtatt ggctttcttg
      961 tttcttttgt tttgccacaa aaatggacta gttctgctgt ggttactcct gctgagtctg
     1021 ttcaatggag tgaattaaat aaggagcttt ccaagcttca tgttctggga gtggattttg
     1081 atatcaatcg tgatagcgca tttgctctgt ttattaagaa atttcagtcc gttaattcgt
     1141 tgaatgagta tatgagatca tctccttatg ttatggagat tattaaaaag aataacatta
     1201 gcgctctcga actgcatcgt gcaattgttg agttgagtaa aaatatgaag tcgattgatg
     1261 ataatgccag taagaaaaac gacaagtcat cattgtatgt atcatggact ctgagtttta
     1321 ctgctccaac aagtaaagaa gctcacgatg tgttgtctgg gtatattaat tatgtttctt
     1381 cccttgttgt aagggatttg atggaagata taagaaataa actagaagtt aaaactaatg
     1441 ttgaaaaaga aattcttgca ctggatgaga taaaaattag aaaccagctg aatgcagata
     1501 ttcgacgcct caattattca ctggaggttg ctaatgcggc tggaataaaa aaacctgtat
     1561 acagcaatgg tcagattatg aaggatgacc cagattttcc tgtggctctc ggttctgatg
     1621 gtatagcaac taaattgaac atcaaaaaat caatcaagga tgtttcggaa ttgagtgggg
     1681 agttgcgaaa tcgtcaatat gttgtgaatc aattggttgt ggcgaaagtg ggggatgttg
     1741 attttatgcc atttcagtat caattgagcc caacgttacc ggtcagaaag gatgggccaa
     1801 gtaatgccat aattgtaatt ctttcttctg tcattggagg gatgcttgca tgtggctata
     1861 tacttttacg tcatgccatg gaggccagaa agatgagtgt gattgagtaa aagttgtgct
     1921 gatgcatcat tttataagca acttttataa aaagttcaaa ggatcttctt gagatcattc
     1981 ccttacagcc gtagtcttct actctagaaa acgaaaaaac cacctgggga ggtggttttt
     2041 cgagggttca gtaagttggg gaacttctga accgtggtaa cagtcttgtg cgagacatgt
     2101 caccaaatct gtccttctag tgtagcctca gtaaggctgc cacttcaaga actctcaata
     2161 catctctcgc acatcctgtt taccagtggc cgctgccaat ggcgttaagt cgtgtcttac
     2221 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     2281 ttcgtgcaca cagcccagct tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg
     2341 aatgagacga acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag
     2401 gagagcgcac gagggagcca ccagggggaa acgcctggta tctttaagtc ctgtcgggtt
     2461 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     2521 gaaaaacgcc accggcgcgg ccttttttcg gggcctcttt tttgctcaca tgttttttct
     2581 ggtgttatcc cctgattctg tggataaccg tatatccgtc tttgcgtgag cagacaccgc
     2641 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccg
     2701 gaagctgcat tttctcctta cgtatctgtg cagcatttca cacctcatat gtcgctgttc
     2761 tctcaccatc tgctctgccg cctcataatt aagccagtat acactccgtt atcgctacgt
     2821 gactgggtca tggctgcgcc ccgacacccg ctaaaacccc ctgacgcgcc ctgacgggct
     2881 tgtctgctga cgaaaacgcc cacgggaaag ccgtgaccgc ctccgtcagc ttcgttatgt
     2941 tcctgtttca ctttcggcct caaagcgaat ctggatgctg ttctggagtt cttctgcgag
     3001 ttcgtgcagt cgtttgcaca tggccgcctg ttcgtcggca tccagagcat ccagtttttc
     3061 aagcagtgtc aggctctggc ttttgataca tcgcgccata ttcagcgcag tttgttgtta
     3121 tttgttcacc ttccgttgtt ctccgtcag
//
LOCUS       DAJTSI010000264         3097 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000264 DAJTSI010000000
VERSION     DAJTSI010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.269"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(104..1813)
                     /locus_tag="OO327_004048"
     CDS             complement(104..1813)
                     /locus_tag="OO327_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCR6020376.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2245..2964)
                     /locus_tag="OO327_004049"
     CDS             complement(2245..2964)
                     /locus_tag="OO327_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCR6020377.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT          953 a          588 c          519 g         1037 t
ORIGIN      
        1 gccaattgag gtggttgcct gaaagttggc agttacccgg tcactacacc ttgttggagt
       61 gaacatatca aaaacaggta attacacaat ttaaattaca gtctcataaa aaatgattaa
      121 atctatttat tatttcaata aacgtttttt tcatcttctt tgaaaattgt tctgctatag
      181 tatccggttc cagattgttc tcttttatta ttctacaata ctctaatatt gtttctttgt
      241 ctctatttac tacagataca aatacaccag gaataccatc tttattagtt gcggacagca
      301 tttcgaccaa tcgtcttgtg gtaaaactat agttttttag aatattacaa aaggcacgta
      361 ttgtttcggt atggccatta taaaatgcca taaaaagtcc tgatgtaccg ttgggatttt
      421 ttatccataa taattcatct atatattcct ggtgaggggg gagtattttt agtatatctc
      481 catacgccat aatggccttt gtgtgcccat tttgaagtgc cataaacaac ccatgcttta
      541 ctttcaataa ttctttaatt tgttcactac taagcatttt cttattaagc agacttccga
      601 acatacgaat actatctgca tgtccattct gcaatgcaaa aaataaccca ggtaaatcat
      661 ggctgtcttt cgcagctaat agatataatc tcttatcgga agttaacggg gttttaaaga
      721 ctatttcccc ataggctttt attgtgtcta catgtccgtt cataaaagac atgcataaac
      781 ccggcacatt gtttgcccct accgcagcaa gcaatgtaag tattgtttct tgactaagat
      841 tgcacatatt taatatctta ccatatactt gaataatgtc tgaatatccg ttttgaagtg
      901 ctatataaaa tgcaggagta ccatcagagc ttctcgtggc cagaaggttc accagtttag
      961 attgagaaat ttcaccattt ttgtattttt ccatcagtat aaataatact tctgtcagat
     1021 tctttgttat cgcatgatat ataactctgg aattgatcaa ttcatctggt aaaagtttca
     1081 ctgtgtttgt tagaggggaa ttatgcctat cttcaaatat tacctgcccg ggttgctcca
     1141 gaccataaat tttccaataa aaatcatcaa aaaaatcctt agcggataat tgactcagtt
     1201 cgaaatggct atcacaagta aatacagtac gtttatgagt aacagttcga ttaggatcga
     1261 aaaactgaac aacccatttt tcactttctt gtacgcgctt tattttcaac cctaacgcca
     1321 tttggtggtt taatgtgcat gctgataatg ttctgacaaa gtctccattt tttttcatgt
     1381 tataaaaaaa attagtaata actgttcccc actttttatt ttccacaaaa taaatataag
     1441 ctggtgattt tgaaaaatct tccgtttttt ccaaacatac attttttgca atagcatcag
     1501 gggtgctaag aagctgatag tttaccttcc catttgtttc cataaaattc atttcccaat
     1561 aggaagctag gtgacggcaa actatttgtc ttccatatat atcttttgcc actccattta
     1621 tatttacctc cacatttctc ttttctgaaa aataaggtgc tgttttattc ttaaactgaa
     1681 ttagttcact ggagtttgag accctcataa tagaacttgt tattgttcta ttatctctgt
     1741 taacagacat atctgttgat aacgaatttt ttgttgaaaa tatactagat gaaaatgttt
     1801 tatttaacgg catagaagac aaccatagag atgttataat actattagat tgcagttact
     1861 aatagtaaaa gcctccttac aaggaggtag cttattcaga atgctcctat aagatagcga
     1921 gctcgtcaac gatacagatg atctgattat attgtgcaaa aggagaacag gaaaataggc
     1981 actggaaaag atgaaggcct gatgggagca ttaactaatg caaaatccga caggttaata
     2041 tcccgaaaag caatcaaacg actcaaacag gagatgcacg ctgaaatggt ggacaactgt
     2101 tcggatttca gatgagtagc tcagcagttt atgagccgaa tgctctgtca cgggcaggaa
     2161 tcaggctgca tacgcagtgc caaatctaat cgtagttatt ctaaccagtt ttcaagctac
     2221 aaggtggtgt agctggcatc ttctctactc tatcatcaaa cgataaaatg gttcctctcg
     2281 taacatttgc tcttgccttt cacttccatc tcgatcgctt cgtagttcat tacgatagct
     2341 gacatatttc cagtcttcag gtctaacatc tgacgccgga tactccccgg gagcgatttt
     2401 gcttcttaat agattagact cgagacacat tataaattgt cgtattttat gaagtaataa
     2461 agcactatat tgcgagcatt cctgatctga ttttacatat agcgtaaact gagctcctat
     2521 ccccacacga gattgctgag aaactcgatt catatcagtt atcttccatt tatctatagg
     2581 actgtcctct gaaaataata aaccagacaa aatttggaat gccagaggaa cttgctccct
     2641 agctatacta atatgaaact tgtccccaac aaaatccccc ttactctgag gattttcacg
     2701 acatgcatga ataaacacat ctccactatg gttcacaatc tggaatcctt ggtacatgac
     2761 agaaaaagat tgacatatat cagagtaagt tggcacgttc gatgctactt tgaaatagta
     2821 cgctgggtat tgccgtattt ggttgtacaa tatattgaag ttatttttca atctttcatt
     2881 cgcagaaagc atctgcggta tttcagacct cacaacattc aaagaatcta aatttagttt
     2941 tagacagggc ttttttatgg gcatagaaaa cgtcctctat aaaatagata tattatctta
     3001 tattgattgt ttatcatctt ttataaaacg atacattata tgtaatgtat gtaagcgtaa
     3061 gggtcctctc cgaacatggt gggaaggctt agaacgc
//
LOCUS       DAJTSI010000265         3080 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000265 DAJTSI010000000
VERSION     DAJTSI010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.270"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            2..256
                     /gene="yoeB"
                     /locus_tag="OO327_004050"
     CDS             2..256
                     /gene="yoeB"
                     /locus_tag="OO327_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCR6020378.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            339..1163
                     /locus_tag="OO327_004051"
     CDS             339..1163
                     /locus_tag="OO327_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6020379.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            1209..2192
                     /locus_tag="OO327_004052"
     CDS             1209..2192
                     /locus_tag="OO327_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6020380.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            2353..2415
                     /gene="yoeI"
                     /locus_tag="OO327_004053"
     CDS             2353..2415
                     /gene="yoeI"
                     /locus_tag="OO327_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR6020381.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            2405..>3080
                     /gene="plaP"
                     /locus_tag="OO327_004054"
     CDS             2405..>3080
                     /gene="plaP"
                     /locus_tag="OO327_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR6020382.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSE"
BASE COUNT          749 a          713 c          807 g          811 t
ORIGIN      
        1 agtgaaacta atctggtctg aggaatcatg ggatgattat ctgtactggc aggaaacaga
       61 taagcgaatt gttaaaaaga tcaatgaact tatcaaagat acccgcagaa caccatttga
      121 aggtaagggg aaaccagaac ccctgaaaca taatttgtca ggtttctggt cccgacgcat
      181 tacagaggag caccgtctgg tatacgcggt tactgatgat tcactgctca ttgcagcatg
      241 tcgttatcat tattgaacag attcattcat agaatgaatt gtcagttttg atacgctagc
      301 gtagccttat agatctaagg tgtatcagga gataacggat gaaaaaggtc gcaattgtcg
      361 ggttagggtg gttaggtatg ccgctggcga tgtcactttc agcgcgaggc tggcaagtca
      421 ccgggagtaa aaccacacaa gatggtgtcg aagcggcccg aatgagtggc attgatagct
      481 atctgcttcg catggagcct gagttagttt gcgattctga cgatctggat gcccttatgg
      541 atgccgatgc gctggtcatt acgcttccgg cacgtcgtag cggccccggc gatgagttct
      601 atttacaagc ggtacaagag ttagtggata gcgcgctggc ccatcgtatt ccccgaatta
      661 tttttaccag ctcaacatct gtctatggcg acgcgcaagg cacggttaaa gaaactaccc
      721 cgcgtaatcc ggtaaccaac agtggacgag tattagaaga actcgaagac tggctgcaca
      781 atttacccgg tacttcggtc gatattctgc gtcttgcggg cctggtcgga ccgggacgtc
      841 atccgggacg cttctttgcc ggaaaaaccg cccctgatgg tgaacatggt gttaatttag
      901 tccatttaga agatgttatt ggcgctatca ctctgttgtt acaggcacct aaaggcggac
      961 acatctataa tatatgtgcg ccagctcacc ctgcgcgtaa tgttttctat ccgcagatgg
     1021 cccgtttact ggggctgcaa cctccgcagt tcagaaatag tctggacagc ggcaaaggta
     1081 agattattga tggcagtcgg atttgtaatg aactgggatt tgaataccag tatcccgatc
     1141 cgctggtaat gccgttggag taaacgatca cgccagcagg acgccgcatg cactgcaagg
     1201 gggcgtgtat gaaaccactg ctggatgtgt tgatgatcct cgatgccata gaaaaagagg
     1261 gcagttttgc ggcggcgtca gccaaactct acaagacgcc gtccgctctg agttacaccg
     1321 ttcacaagct ggaaagcgac ctaaatattc aattgcttga tcgtagcgga caccgcgcta
     1381 aattcacccg caccggaaaa atgttattag agaaagggcg ggaagttctg cataccgtgc
     1441 gagaactgga aaagcaggcg atcaaactgc atgaaggctg ggaaaacgag ctggtgattg
     1501 gcgtggacga cacgtttcct ttttctctgc ttgcgcctct cattgaagct ttttatcaac
     1561 atcatagcgt gacgcgcctg aagtttatca atggcgtact cggtggttcc tgggatgccc
     1621 tgactcaggg gcgagcggat attatcgttg gcgcgatgca tgagccacca tcttccagtg
     1681 agtttggttt ctcgcggttg ggcgatcttg aacaagtctt tgcggtcgcg cctcatcatc
     1741 cgttagccca ggaagaagaa ccgttaaacc gtcgtatcat taaacgatat cgggcgattg
     1801 tggtggggga tactgcgcag gcaggcgctt ctacggcctc acaacttctc gacgaacaag
     1861 aagccattac cgttttcgat tttaaaacca aactggagct gcaaattagc ggccttggct
     1921 gcggctattt accacggtat ctggcgcaac gttttctcga tagtggcgcg ttaatcgaga
     1981 agaaagtggt cgcccaaact ctctttgaac ctgtctggat tggctggaat gaacagaccg
     2041 cagggcttgc cagcggctgg tggcgagatg aaattttagc aaatagtgcg atcgccggtg
     2101 tttatgcaaa atctgatgac ggaaaatcag ccattcaaag aaaaattatt atgacaagcc
     2161 tctcattctc ttgtcatttc ccccccattt aggcacaatg cgccgctgtc aaaaaatgat
     2221 taaaaaccga cgtttaatta gcgtcggtta ttttttgctt caaaccaatc attcatacca
     2281 agaggccggg cttcgtaccg gatagatatt tactaaaaat cgacagttgt tgtcgctgag
     2341 gaatccaaaa aaatggggca attttttgct tacgcgacgg ttatcaccgt aaaggagaat
     2401 aaccatgtcg cataacgtta ctccaaacac ctctcgcgtg gaattgcgta aaacgcttac
     2461 gttagttccg gttgtaatga tgggtctagc ctatatgcag ccgatgacgc tgtttgatac
     2521 atttggtatc gtttcaggcc tcacggatgg tcatgtgccg acagcctatg cgttcgcatt
     2581 gattgcgatc ctgtttacgg ctctgagcta cgggaagctg gttcgccgct atccttctgc
     2641 tggctctgca tacacttacg cccagaaatc cattagcccg actgtcggct ttatggtggg
     2701 ttggtcttct ctgctcgact atctgttcgc gccgatgatc aacattctgc tggcgaaaat
     2761 ctattttgaa gctctggtgc cttccattcc atcgtggatg tttgtggtgg cgctggtggc
     2821 ctttatgacc gcctttaacc tgcgtagtct gaaatccgta gcgaacgtca acactgtaat
     2881 cgtcgtgttg caggtagtgc tgatcgcggt gattctgggc atggttgttt atggcgtatt
     2941 tgaaggtgaa ggcgctggta cgctggcgag cacgcgtccg ttctggtctg gcgatgcgca
     3001 tgttatcccg atgattaccg gggcgacaat cctgtgcttc tcctttaccg gctttgacgg
     3061 catcagcaat ctgtcggaag
//
LOCUS       DAJTSI010000266         3069 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000266 DAJTSI010000000
VERSION     DAJTSI010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.271"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..498
                     /locus_tag="OO327_004055"
     CDS             <1..498
                     /locus_tag="OO327_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6020383.1"
                     /translation="DGEILKSACEINDSDKKIEVALGHYNAEQFRSVGDRSPKIPFTI
                     PLVNCPVTGWEHDNGNVEASFRLWLETRDNGTVPNFPNLAKVGSFAGTATTGVGIRID
                     DAESGNLMPLNAMGNDNTIYQIPADSNGIVNVDLIAYYVSTVEASEITPGEADAVVNV
                     TLDYR"
     gene            578..1273
                     /locus_tag="OO327_004056"
     CDS             578..1273
                     /locus_tag="OO327_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6020384.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEASDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1302..>3069
                     /locus_tag="OO327_004057"
     CDS             1302..>3069
                     /locus_tag="OO327_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6020385.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLLSLN"
BASE COUNT          888 a          718 c          684 g          779 t
ORIGIN      
        1 gatggcgaga ttttaaaatc cgcttgtgaa atcaatgact ctgataagaa aattgaagtt
       61 gctcttggtc actataatgc cgaacagttt cgttctgttg gcgatcgtag cccaaaaatt
      121 ccattcacta ttcctttagt taactgccca gtgactgggt gggagcacga taacggcaac
      181 gttgaagcgt ctttccgttt atggctggaa acgcgggata atggcaccgt ccctaacttc
      241 cctaatctgg caaaagtggg ctcttttgct ggcacagcga caacaggtgt gggtatccgt
      301 attgacgatg cggaaagcgg aaaccttatg ccactgaatg ctatggggaa tgataacacg
      361 atttatcaga tcccagcgga ctccaatggt attgtcaatg ttgacctcat cgcttactac
      421 gtatcaaccg ttgaagcatc agaaatcacc ccaggggaag cagacgctgt cgttaacgta
      481 acgctggatt accgttaata cgttacggcg ttatctgacc tttcagataa cgcccttttc
      541 cttcctcttt ctcgttgtat caggttgaaa aataactatg tcaaaacgaa cattcgcggt
      601 gatattaacc ttgttgtgta gcttctgtat cggccaggcg cttgcagggg gtatcgtttt
      661 acagcgaacg cgagtgatct atgatgccag ccgcaaagag gctgcgttac ctgtcgcaaa
      721 caaaggcgca gaaacgcctt atttactgca atcatgggta gataatatag atggtaaaag
      781 ccgtgcccca tttattataa ccccaccgct atttcgtctt gaggctagcg atgactcatc
      841 actgcgaatt attaaaacag ctgataacct gcctgaaaat aaagagtcgc tgttctacat
      901 taatgttcgt gccattccag caaagaaaaa atcagatgat gttaatgcta acgagttgac
      961 gctggtattt aaaacgcggg tcaaaatgtt ttatcgcccc gcacacctga agggacgggt
     1021 aaacgatgcg tggaaatcac tggaatttaa acgtagtgac cattcactca atatatataa
     1081 cccaactgaa tattacgtcg tatttgccgg actggcagtc gataaaaccg atctcacaag
     1141 caaaattgaa tatatcgcgc ccggagaaca taaacagtta ccacttccag catctggcgg
     1201 aaagaacgtg aaatgggctg cgatcaacga ttatggcggc agttccggga cagaaactcg
     1261 tccactgcaa taaaaaatat aaaaacacag gccatcaggg aatgccacaa cgacaccacc
     1321 agggacataa acgcacaccg aaacagttgg cgctcattat caaacgttgt ttgccgatgg
     1381 tgctcactgg cagcggcatg ctttgcacta ccgctaacgc cgaagagtat tatttcgacc
     1441 ccattatgct ggaaactaca aaaagtggta tgcaaacaac cgatctgtca cgtttttcaa
     1501 aaaaatacgc acaactacca ggaacttatc aggttgatat ctggctgaat aaaaagaagg
     1561 tttcacagaa aaaaattaca tttaccgcca atgcagagca acttctgcag ccacagttta
     1621 cggtagaaca actacgtgag ctgggtatta aggtggatga aatcccggcg ctggctgaaa
     1681 aagatgacga tagcgtgatc aactcgcttg aacaaatcat tcccggtaca gctgctgaat
     1741 ttgatttcaa tcatcagcga cttaatttga gcattcccca aattgcactg taccgtgatg
     1801 caagaggtta cgtctcccct tctcgttggg acgatggtat accaacgctg tttaccaact
     1861 actcgtttac aggttctgat aaccgttacc gccagggcaa tcgtagccaa cgacagtacc
     1921 tgaatatgca aaatggtgct aattttggcc cctggcgatt acgtaactat tctacgtgga
     1981 cacgcaacga tcaggcgtca agctggaaca ctatcagtag ttatttacaa cgtgatatca
     2041 aggcgttgaa gtctcagttg cttctgggag aaagcgccac cagcggcagt attttttcca
     2101 gctacgcctt tactggcgtg caactcgctt ccgacgataa tatgttgcca aacagccagc
     2161 gcggatttgc cccaacggta cgcggtatcg caaacagtag tgcaatcgtg actatcaggc
     2221 aaaatggtta tgtgatctat caaagcaacg tgccagcggg tgcctttgaa attaacgatc
     2281 tctacccctc ttccaacagc ggcgatttag aagtcacgat tgaagaaagt gacggtacac
     2341 aacgtcgctt tatccagcct tattcttcat tacccatgat gcagcgacct gggcatctaa
     2401 aatatagcgc gaccgctgga cgctatcgcg ctgatgcaaa cagtgatagc aaggaacccg
     2461 aatttgctga agccacggca atatatggtt tgaataatac ttttacgctg tatggcggcc
     2521 tgctcggttc tgaagattat tatgcgctgg ggatcggtat cggcggcaca cttggcgcac
     2581 tgggcgcgtt gtcgatggat atcaacagag ctgacaccca attcgataac cagcactctt
     2641 ttcatggcta tcaatggcgt actcagtaca tcaaagatat cccggaaacc aacaccaata
     2701 tcgctgttag ctactatcgc tataccaacg atggctattt tagttttgat gaagccaata
     2761 cccgcaattg gggctataac agtcgccaaa aaagtgaaat tcaattcaac atcagccaga
     2821 caatatttga tggggtaagt ctgtatgcct ccggttcaca gcaagactat tggggcaata
     2881 acgagaaaaa cagtaatatc tctgttgggg tttccggcca gcaatgggga attggttaca
     2941 gcctgaatta tcaatacagc cgctacactg atgaaaataa tgaccgtgca ctctctttga
     3001 atctcagtat tccgttagaa cgctggtgga tttgccccta tatttccaga catctgttat
     3061 cacttaacc
//
LOCUS       DAJTSI010000267         3065 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000267 DAJTSI010000000
VERSION     DAJTSI010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.272"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            112..1167
                     /gene="phoE"
                     /locus_tag="OO327_004058"
     CDS             112..1167
                     /gene="phoE"
                     /locus_tag="OO327_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR6020386.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            complement(1206..1607)
                     /gene="crl"
                     /locus_tag="OO327_004059"
     CDS             complement(1206..1607)
                     /gene="crl"
                     /locus_tag="OO327_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR6020387.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(1665..2909)
                     /gene="frsA"
                     /locus_tag="OO327_004060"
     CDS             complement(1665..2909)
                     /gene="frsA"
                     /locus_tag="OO327_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR6020388.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(3001..>3065)
                     /locus_tag="OO327_004061"
     CDS             complement(3001..>3065)
                     /locus_tag="OO327_004061"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="catalyzes the conversion of guanine, xanthine and,
                     to a lesser extent, hypoxanthine to GMP, XMP and IMP,
                     respectively; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6020389.1"
                     /translation="TWIEQPWDMGVVFVPPISGR"
BASE COUNT          819 a          747 c          746 g          753 t
ORIGIN      
        1 aatatcatta atctgtaata tatctttaac aatctcaggt taaaaacttt cctgttttca
       61 acggggctct cccgctgaat attcgcgcgt taattaaaat cagaaatgaa aatgaaaaag
      121 agcactctgg cattagtggt gatgggcatt gtggcatctg catctgtaca ggctgcagaa
      181 atatataata aagacggtaa taaactggat gtctatggca aagttaaagc catgcattat
      241 atgagtgata acgccagtaa agatggcgac cagagttata tccgttttgg ttttaaaggt
      301 gaaactcaaa ttaacgatca actgactggt tatggtcggt gggaagcgga gtttgccgga
      361 aataaagcgg agagtgatac tgcacagcaa aaaacgcgtc tcgcttttgc cgggttgaaa
      421 tataaagatt tgggttcttt cgattatggt cgtaacctgg gcgcgttgta tgacgtggaa
      481 gcctggaccg atatgttccc ggaatttggt ggcgattcct cggcgcagac cgacaacttt
      541 atgaccaaac gcgccagcgg tctggcgacg tatcggaaca ccgacttctt cggcgttatc
      601 gatggcctga acttaaccct gcaatatcaa gggaaaaacg aaaaccgcga cgttaaaaag
      661 caaaacggcg atggcttcgg cacgtcattg acatatgact ttggcggcag cgatttcgcc
      721 attagtgggg cctataccaa ctcagatcgc accaacgagc agaacctgca aagccgtggc
      781 acaggcaagc gtgcagaagc atgggcaaca ggtctgaaat acgatgccaa taatatttat
      841 ctggcaactt tctattctga aacacgcaaa atgacgccaa taactggcgg ctttgccaat
      901 aagacacaga actttgaagc ggtcgctcaa taccagtttg actttggtct gcgtccatcg
      961 ctgggttatg tcttatcgaa agggaaagat attgagggca tcggtgatga agatctggtc
     1021 aattatatcg acgtcggtgc tacgtattat ttcaacaaaa atatgtcagc gtttgttgat
     1081 tataaaatca accaactgga tagcgataat aagctgaata ttaataatga tgatactgtc
     1141 gcggttggga tgacgtatca gttttaatga atattgccgg atgtgatgca tccggcacat
     1201 ttcactcacg ccgttaactt caccggttca tcacgaaaat cattcgccgg ttccagcttc
     1261 agattcagcg tcgtcagcag ttcacgcagc ttctcgtgaa actcacgcag ggtgtgctcc
     1321 agtcgttcaa ccacttcagt atcttttacc ggaacactct tccagtcgcc tgctttatcg
     1381 aacagaccaa actggtagct gtaggtaaaa cgggattcct gcgcttccag ctccatccac
     1441 cagccccaga attcacgcac ttccggtgcc ggtttcacgt tgacgcatac agccagacaa
     1501 tcgaaaaaga atcgattatc ttcgcactta ccttcacgaa tatacgggcc tagtgcggta
     1561 aattttttga tcaatctgct cttcgggtgt ccactcggta acgtcattgc tatctcctgt
     1621 tgtgatgcaa ctgttttacc aaattggcaa aatttagcaa atttttaaca caagcgtttt
     1681 tcgatccagc cggtgatttc ctgaagacct ttgtcaaaat tccgatacac cgggttaaat
     1741 gggatctcta ataatttacc gtcagcagat gatgaggtga ttaagcgtga gtcctcttcc
     1801 gggctgaacg gatcgttctt ccagtagcct gataacattg gcgttgggca gcgacgcccc
     1861 agcaatcctt gcacttttaa tgaatagcga ttcagctcca cgcgtaacgc ttcgtcggaa
     1921 gcatcatgca tccccaaacg actcgccaga acgtcaagat acatttccgg cacctgttgc
     1981 tggcacttaa aatcactcaa cagggtatga actaccggac caagacaggc aaccgctttc
     2041 agacgcggcg attcaaggta tgccagacgc acggcaacgt tagcgccgaa acggaaacca
     2101 aaggccgcga cgcgagtgtg atccacccac ggtacgttag gcagcgcctt taagacgtgc
     2161 tgatgcaaca ggctggagtc ctgggtgagc ttccattttg aagaaaagcc caccgacggc
     2221 atatcaatag tcagcatcgc aatgccgcgc ggcgcaaaat aacgttcata caggctgtaa
     2281 tagtccgtct gcatcgcatc cagaccacca cacattaata ctgtcgggaa cgggccatcg
     2341 cctttcggca tatgcaaaaa gccggtgatg ggcgcaccgc cgggtacggt aaactccatc
     2401 tgccgcatcg tgcccggcag acgctgggcg gcctcttcat aggcgcggtt tgataaagcc
     2461 tgcgcttgct cggccaggtc atctcctttc agatgaggat aggcggcaat gttgtacaac
     2521 gtagccgcat gcagccagtg tcgaccactg agttgcggat cttcttccgt acaggctttt
     2581 tgttgccaca ccatcgcctg ggtggcccac tcataaatcc agttgccacc acggtagcca
     2641 atcacggtat cgtataaatc atcgtcggta cgttcagcat cgctcatcac aatacgtgcc
     2701 tggacgtcga ggatttcacg tgggtcaata ccgcgccaga tccacatcag acggttaatc
     2761 atgcgatacc agtgagggat ggttttacca tcaagagccg actgcacagg cggttgtgcg
     2821 ccgtgattaa agcggcggac tagcgtcgag gtttcaggat gtttaaagcg gggtttgaac
     2881 agggtttcgc tcaggtttgc ctgtgtcatg gatgcagcct ccagaatact tactggaaac
     2941 tattgtaacc tgcctgaagt taaaaagaac aacgcccggc aatgccaggc gttgaaaaga
     3001 ttagcgaccg gagattggcg ggacgaatac gacgcccata tcccacggct gttcgatcca
     3061 ggtat
//
LOCUS       DAJTSI010000268         3065 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000268 DAJTSI010000000
VERSION     DAJTSI010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.273"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..197
                     /locus_tag="OO327_004062"
     CDS             <1..197
                     /locus_tag="OO327_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR6020390.1"
                     /translation="LVEGGIDLKDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQ
                     RGVNVFIQDVPGDQKEQIPD"
     gene            236..1039
                     /gene="agaC"
                     /locus_tag="OO327_004063"
     CDS             236..1039
                     /gene="agaC"
                     /locus_tag="OO327_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HCR6020391.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1029..1820
                     /gene="agaD"
                     /locus_tag="OO327_004064"
     CDS             1029..1820
                     /gene="agaD"
                     /locus_tag="OO327_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HCR6020392.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1821..2576
                     /locus_tag="OO327_004065"
     CDS             1821..2576
                     /locus_tag="OO327_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HCR6020393.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
     gene            2977..>3065
                     /locus_tag="OO327_004066"
     CDS             2977..>3065
                     /locus_tag="OO327_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6020394.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVF"
BASE COUNT          797 a          683 c          726 g          859 t
ORIGIN      
        1 aattggtaga aggtggcatt gacctgaaag atgtcaacgt cggcaatatg catttctcgg
       61 aagggaaaaa gcaaatcagc agtaaagttt atgtcgatga ccaggatctc gcggacttac
      121 gttttattaa acaacgtggc gtgaatgttt tcattcagga cgtccctggc gatcaaaaag
      181 aacaaatccc tgactaaatc taaaatcgcc ttaatattgg tttgaggtaa taaaaatgca
      241 tgaaataacc ctacttcagg gattatccct agcggcgtta gttttcgttc tggggattga
      301 tttttggctg gaagccttat ttttattccg cccgataatc gtttgtaccc taactggcgc
      361 tattctcggt gatattcaga ctggcttaat taccggtggt ctgacagagt tggctttcgc
      421 cggattaacc cctgcaggtg gtgttcagcc gcccaacccg attatggcgg gtctgatgac
      481 caccgtcatt gcatggtcta cgggcgttga tgccaaaaca gcaattggtc ttggcctgcc
      541 gtttagtttg ttaatgcagt acgtcattct gttcttctat tccgctttct cattatttat
      601 gaccaaagcc gataaatgcg cgaaagaggc ggatacggca gcgttttccc ggcttaactg
      661 gacaacgatg ctcatcgtcg cttcagcgta tgcggtgatt gctttcctct gtacttacct
      721 ggcacaaggg gcgatgcagg cgctggtgaa agcgatgccc gcctggttga cccacggctt
      781 tgaagtggct ggcggtattc tgcctgccgt tggttttggc ttgctgctgc gcgtaatgtt
      841 caaagcgcaa tatatccctt acctgatcgc cggtttcctg tttgtttgct acatccgggt
      901 cagcaacctg ttgccggttg ccgtactggg cgcaggcttt gcggtgtatg agtttttcaa
      961 tgcgaaatcc cggcagcaag cgcaaccgca gcccgttgcc agtaaaaatg aagaagagga
     1021 ctacagcaat gggatctgaa atcagtaaaa aagatatcac ccgtctgggc tttcgttcgt
     1081 cgctgctgca agcgagcttt aactacgaaa ggatgcaggc gggcggtttt acctgggcga
     1141 tgttgccgat cctgaaaaag atttataagg acgacaaacc gggcttaagc gcggcgatga
     1201 aagataacct cgaatttatt aatacccacc cgaatctggt cggattcctg atggggttat
     1261 taatttcgat ggaagaaaaa ggagaaaacc gcgacaccat taaaggcctc aaagtggcac
     1321 tgtttggccc aatcgccggg attggcgatg cgattttctg gtttactttg ttgccgatta
     1381 tggcgggaat ttgctcatca tttgccagcc agggaaacct gctggggccg attctatttt
     1441 tcgccgttta cctgcttatc tttttcctgc gcgtcggctg gacccacgtc ggttattcag
     1501 tcggcgtgaa ggcgatcgat aaagtgcgag agaactcgca gatgattgct cgctcggcaa
     1561 ccatcctcgg gatcacggta atcggcgggc tgatcgcttc gtatgtgcat attaacgtgg
     1621 tgacatcgtt tgccatcgac agtacccaca gcgtcgcgct gcaacaggat ttcttcgata
     1681 aagtcttccc gaacatttta ccgatggcct acaccctgct gatgtattac ttcctgcggg
     1741 tgaaaaaagc gcatccggtg ctgttaatcg gcgtgacttt tgttctctct attgtttgtt
     1801 ccgcattcgg cattttgtaa atggaacgag gcactgcttc tggcggtgcc tctttactta
     1861 aggaatttca tcctgtgcaa acccttcagc aagttgaaaa ctatacggcg ttaagtgaac
     1921 gagccagcga atatttattg gctgtgatcc gtagcaaacc ggatgccgtg atttgcctgg
     1981 cgaccggagc cacgccatta ctgacgtatc attatctggt agaaaaaatc caccagcagc
     2041 aggttgatat cagccagctc accttcgtga agctcgacga atgggtggat ctgccattaa
     2101 cgatgcctgg cacctgcgaa actttcctac aacagcatat cgtgcagccg ctggggctac
     2161 gtgaagacca gctcatcagc tttcgctccg aagagataaa tgagacagag tgcgaacggg
     2221 taacgaacct gattgcgcgc aaaggcggtc tggatttatg cgttctcgga ttggggaaaa
     2281 acggtcatct tgggctgaac gaaccgggag aaagcctaca accggcctgc catatcagtc
     2341 aacttgatgc cagaacacag caacatgaga tgttaaaaac cacgggtcgc cccgtgactc
     2401 gtgggatcac cttaggcctg aaggatattc tcaatgcccg cgaggttttg ttattggtga
     2461 ctggcgaagg aaagcaggat gcgacagaac gttttctcac ggctaaagtc tccaccgcta
     2521 tcccggcttc atttttatgg ctgcacagta actttatttg tttaattaat acctaaccag
     2581 atgaaaaatt tgctcaaaat ttttaaatta acttattcaa cagtcacgca ttatattaaa
     2641 taacatttga ctgggttgaa cataacgcca atagcaaaag gagttatgtt caacaattta
     2701 attttagata tacatataac ttgttctatt aataaatcac ctcgtattct gactcggccc
     2761 ctggcgcagc aaagtcaggc atttatactc tttttaacac atagaaaatt catatcaata
     2821 taataaaaac ataaaatatt tgcatataca tataatctta aagtttagtc tatttaatgt
     2881 tcaatgaaat atttctacct gtataatctt taaagatgtt gaacatatat tcacattaaa
     2941 tatgattatg tacttgttac aaggataagg ttataaatga ataaagttac aaaaacagct
     3001 attgtgggct tacttgcgct tttcgcaggt aatgccgctg caaccgatgg tgaaatagtt
     3061 tttga
//
LOCUS       DAJTSI010000269         3060 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000269 DAJTSI010000000
VERSION     DAJTSI010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.274"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..657)
                     /gene="ipaH9.8"
                     /locus_tag="OO327_004067"
     CDS             complement(<1..657)
                     /gene="ipaH9.8"
                     /locus_tag="OO327_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR6020395.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     H"
     gene            complement(919..1014)
                     /locus_tag="OO327_004068"
                     /pseudo
     CDS             complement(919..1014)
                     /locus_tag="OO327_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1050..1355)
                     /gene="ccdB"
                     /locus_tag="OO327_004069"
     CDS             complement(1050..1355)
                     /gene="ccdB"
                     /locus_tag="OO327_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCR6020396.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1357..1575)
                     /gene="ccdA"
                     /locus_tag="OO327_004070"
     CDS             complement(1357..1575)
                     /gene="ccdA"
                     /locus_tag="OO327_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCR6020397.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2111..3060)
                     /locus_tag="OO327_004071"
                     /pseudo
     CDS             complement(2111..3060)
                     /locus_tag="OO327_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          764 a          743 c          736 g          817 t
ORIGIN      
        1 atgggatgat aatgggttat cactaatatg tattgaacat tcattcctca gattaagaat
       61 gctttccggg atatgactta tctgatttct atcaaaaaaa tactctctga caacgggatt
      121 atttccctca gaaaatgctg gtagctcagt gaggaaatta cgggtcgctc tgagattttt
      181 aagagcctgt gggagcgatg gtaaggagac tatttcgtta tagctgatat tcatagtcaa
      241 taacgaatcg ggtaaagctg gcaggttttc cagctcattg tgttgtacct gaagtgactc
      301 cagcgcagga ggtagcacgg gcaattctga taatttattg ctggcggaat ataatttttt
      361 tagcgtaaca ggcagttcag gtaggttagt taattgatta tatgatacat tgagcagcgt
      421 tatctgagct ggtaagttgt caggtagcga ggacagattt aaacggtcca gtcgaagttc
      481 atcggaatta ttgataagac attctttaag tcgggagaca gcctcatcac gctcttcacc
      541 ggggagcgct tgtttttccc atttatccca tgctgaaaag taatcagcat atgtaccgga
      601 aatagtgtta taaaaagaat tttggggcaa tgaaaagtta ttatttatcg gtaacatgat
      661 gggcatccag ttaaagccgg tggacaaatg gctttgttaa aagaaataag taggagtttt
      721 tatgaagttc attccatgaa atgaacttca gcactttttt attataataa ggataaacaa
      781 aattggctgt aaaaaaacga tactgtttca gttaaccgtg aatggcctgt ttgcaccgta
      841 ttgttcttgt ttttcggtta gttaaacatc gtacttcata tttgaacggt aagcggctcg
      901 tcagaaccgt attgatattt actgagaggt cagatcaact ttccaaggca acagatcgcg
      961 tacccggttt gccggccagt cctggatatg ctcaatgacg taacgcagcc attttgtcaa
     1021 cgccacgatt tttgacccta gcctgacatt tatattcccc agaacatcag gttaatggcg
     1081 tttttgatgt cattttcgcg gtggctgaga tcagccactt cttccccgat aacgaagatc
     1141 ggcacactgg ccatatcagt ggtcatcatg cgccagcttt catccccgac atgcaccacc
     1201 gggtaaagct cacgggagac tttatctgac agcaggcggg cactggccag ggggatcacc
     1261 atccgtcgtc cgggcgtgtc aataatgtca ctctgtacat ccacaaacag acgataacga
     1321 ctctctcttt tataggcgta aaccttaaac tgcatttcac cagtccctgt tctcatcggc
     1381 aaaagagccg ttcatttcaa taaaacgggc gatctcagcc atcccctgct gattttcagc
     1441 ctgccagcgt tccgcacgca gacggcgggc ctctttttgc atggccgtat ttaccaggcc
     1501 tgagatattc acgtttgcgg atttaagtaa ctgatagctg tcactgtcta tagtgacagt
     1561 aatacgctgc ttcatgataa gccctcaaaa acatacttat gatatgtata tgagtatata
     1621 ttaatggtac aaaccatcaa tatccataaa aagtacacca ttaattcgct ataagttttt
     1681 aatacaattt tttctgctgc gattttttta tactataatt agcatactta taacaccgta
     1741 agtaaattac atgaaagcag gcacagcgtg atccgatttt tctgcgtata acgattttcc
     1801 ggaagtgtaa tacggttgcg ccccgggccg ggcacccttt tctgttgttg tgtacgggcc
     1861 cacagagaat ggtaccggag gcgggctcag aagtactgag tcgggtgatc gcgtcaggcc
     1921 gcgaagtact gacacgggta ttggtgactg gtgatgaagt actgccccgg gtatgatttc
     1981 aggctgattc aggttctgat tctctgtcag tccgcgtggt tgctggcctg gctgatttcc
     2041 tgttgtcagg cgttcaacaa ccgactgcag gttatagccc ggttcacgaa cacctgacgg
     2101 caggtatcac tcacccggtg aaggtaaatc cctcaagagg cagcaactct gccagtcgct
     2161 cctccggcca ttccggcaga cgcgtcagga cgtccgtcag ccaggcatgt ggctccagac
     2221 cattacgttt tgctgtttcc agaaggctca ttatctgtgc cgcccggttt ccggccatca
     2281 gtgaaccggc gaacagccac gctttccgtc ccataacaac cgggcgaatg gcacgctcgc
     2341 agatattgtt gtccaggggc acagctccgt cacccaggaa gcagctcagt tcatcgcgcc
     2401 ggttcagaat atagttaatc gccttattta atggcccgtc gggcggacag cacggctcct
     2461 gctcttcaag ccaggcaaac aggtcattca ctatcggccg ggagtaccgt tatcgccact
     2521 gccgtatttt ttccacaggg cgctcacgga tgagtttctc aatgcggtaa agacccgcga
     2581 tttttctcag ctccacgcct gcacggggct ccctgctggc cttatacaga tttgcgaagc
     2641 cgcgccctgc gtgactccag caacaggctg atgtgatgcc ggggtgatta tccgccagag
     2701 atttgtacac gctgtatccg tcactcacca gcgtactgcc acaccagcac tgcagccagg
     2761 tctcagggta tctgagggcc cgtccggtct gcgagtcaaa acagacgacc ggcggaccgc
     2821 ttctttcacc gctgacgtaa gcacacagcc agccggagca ggatttaccg cctttccggg
     2881 tatcaaggag cctcagggac gtttcatctg catgaataac atcccgtgtc agaagctcat
     2941 ggcgtagcag tttcgccagc ggggccagtg cagctccggc aacaccgacc atatccgcca
     3001 ttgtactgac ggggagctcc acacccattc gggagaaaat gtgctgctgg cgatacaggg
//
LOCUS       DAJTSI010000270         3059 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000270 DAJTSI010000000
VERSION     DAJTSI010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.275"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            2..2905
                     /locus_tag="OO327_004072"
     rRNA            2..2905
                     /locus_tag="OO327_004072"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          811 a          673 c          954 g          621 t
ORIGIN      
        1 aggttaagcg actaagcgta cacggtggat gccctggcag tcagaggcga tgaaggacgt
       61 gctaatctgc gataagcgtc ggtaaggtga tatgaaccgt tataaccggc gatttccgaa
      121 tggggaaacc cagtgtgatt cgtcacacta tcattaactg aatccatagg ttaatgaggc
      181 gaaccggggg aactgaaaca tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc
      241 cagtagcggc gagcgaacgg ggagcagccc agagcctgaa tcagtgtgtg tgttagtgga
      301 agcgtctgga aaggcgtgcg atacagggtg acagccccgt acacaaaaat gcacatactg
      361 tgagctcgat gagtagggcg ggacacgtgg tatcctgtct gaatatgggg ggaccatcct
      421 ccaaggctaa atactcctga ctgaccgata gtgaaccagt accgtgaggg aaaggcgaaa
      481 agaaccccgg cgaggggagt gaaaaagaac ctgaaaccgt gtacgtacaa gcagtgggag
      541 cacgcttagg cgtgtgactg cgtacctttt gtataatggg tcagcgactt atattctgta
      601 gcaaggttaa ccgaataggg gagccgaagg gaaaccgagt cttaactggg cgttaagttg
      661 cagggtatag acccgaaacc cggtgatcta gccatgggca ggttgaaggt tgggtaacac
      721 taactggagg accgaaccga ctaatgttga aaaattagcg gatgacttgt ggctgggggt
      781 gaaaggccaa tcaaaccggg agatagctgg ttctccccga aagctattta ggtagcgcct
      841 cgtgaattca tctccggggg tagagcactg tttcggcaag ggggtcatcc cgacttacca
      901 acccgatgca aactgcgaat accggagaat gttatcacgg gagacacacg gcgggtgcta
      961 acgtccgtcg tgaagaggga aacaacccag accgccagct aaggtcccaa agtcatggtt
     1021 aagtgggaaa cgatgtggga aggcccagac agccaggatg ttggcttaga agcagccatc
     1081 atttaaagaa agcgtaatag ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg
     1141 ctaaaccatg caccgaagct gcggcagcga cgcttatgcg ttgttgggta ggggagcgtt
     1201 ctgtaagcct gtgaaggtgt gctgtgaggt atgctggagg tatcagaagt gcgaatgctg
     1261 acataagtaa cgataaagcg ggtgaaaagc ccgctcgccg gaagaccaag ggttcctgtc
     1321 caacgttaat cggggcaggg tgagtcgacc cctaaggcga ggccgaaagg cgtagtcgat
     1381 gggaaacagg ttaatattcc tgtacttggt gttactgcga aggggggacg gagaaggcta
     1441 tgttggccgg gcgacggttg tcccggttta agcgtgtagg ctggttttcc aggcaaatcc
     1501 ggaaaatcaa ggccgaggcg tgatgacgag gcactacggt gctgaagcaa caaatgccct
     1561 gcttccagga aaagcctcta agcatcaggt aacatcaaat cgtaccccaa accgacacag
     1621 gtggtcaggt agagaatacc aaggcgcttg agagaactcg ggtgaaggaa ctaggcaaaa
     1681 tggtgccgta acttcgggag aaggcacgct gatacgtagg tgaagcgact tgctcgtgga
     1741 gctgaaatca gtcgaagata ccagctggct gcaactgttt attaaaaaca cagcactgtg
     1801 caaacacgaa agtggacgta tacggtgtga cgcctgcccg gtgccggaag gttaattgat
     1861 ggggttagcg caagcgaagc tcttgatcga agccccggta aacggcggcc gtaactataa
     1921 cggtcctaag gtagcgaaat tccttgtcgg gtaagttccg acctgcacga atggcgtaat
     1981 gatggccagg ctgtctccac ccgagactca gtgaaattga actcgctgtg aagatgcagt
     2041 gtacccgcgg caagacggaa agaccccgtg aacctttact atagcttgac actgaacatt
     2101 gagccttgat gtgtaggata ggtgggaggc tttgaagtgt ggacgccagt ctgcatggag
     2161 ccgaccttga aataccaccc tttaatgttt gatgttctaa cgtggacccg tgatccgggt
     2221 tgcggacagt gtctggtggg tagtttgact ggggcggtct cctcctaaag agtaacggag
     2281 gagcacgaag gttggctaat cctggtcgga catcaggagg ttagtgcaat ggcataagcc
     2341 agcttgactg cgagcgtgac ggcgcgagca ggtgcgaaag caggtcatag tgatccggtg
     2401 gttctgaatg gaagggccat cgctcaacgg ataaaaggta ctccggggat aacaggctga
     2461 taccgcccaa gagttcatat cgacggcggt gtttggcacc tcgatgtcgg ctcatcacat
     2521 cctggggctg aagtaggtcc caagggtatg gctgttcgcc atttaaagtg gtacgcgagc
     2581 tgggtttaga acgtcgtgag acagttcggt ccctatctgc cgtgggcgct ggagaactga
     2641 ggggggctgc tcctagtacg agaggaccgg agtggacgca tcactggtgt tcgggttgtc
     2701 atgccaatgg cactgcccgg tagctaaatg cggaagagat aagtgctgaa agcatctaag
     2761 cacgaaactt gccccgagat gagttctccc tgacccttta agggtcctga aggaacgttg
     2821 aagacgacga cgttgatagg ccgggtgtgt aagcgcagcg atgcgttgag ctaaccggta
     2881 ctaatgaacc gtgaggctta accttacaac gccgaagatg ttttggcgga ttgagagaag
     2941 attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaattag
     3001 cctggcggcc gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacg
//
LOCUS       DAJTSI010000271         2951 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000271 DAJTSI010000000
VERSION     DAJTSI010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.276"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            141..2009
                     /gene="kup"
                     /locus_tag="OO327_004073"
     CDS             141..2009
                     /gene="kup"
                     /locus_tag="OO327_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR6020398.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFSIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2176..2595
                     /gene="rbsD"
                     /locus_tag="OO327_004074"
     CDS             2176..2595
                     /gene="rbsD"
                     /locus_tag="OO327_004074"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR6020399.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2603..>2951
                     /locus_tag="OO327_004075"
     CDS             2603..>2951
                     /locus_tag="OO327_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6020400.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          658 a          701 c          779 g          813 t
ORIGIN      
        1 aagggggggc tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      421 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      481 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      541 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      601 tatttatgat tcaaaagcat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      661 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      721 tgctgcatgc gctgaatccg atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      781 cttttattgc attaggggca gtggtgctgt cgattacggg gggcgaggtg ctgtatgctg
      841 atatggggca ctttggtaag ttctctattc gcctggcgtg gttcaccgtc gtattacctt
      901 ccttgaccct taattacttc ggtcagggag cgttgttgtt aaagaacccg gaagcgatta
      961 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccactgctg atcatcgccg
     1021 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
     1081 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
     1141 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1201 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1261 ccatggtact gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1321 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1381 ctaacctcga taaactgctc tccggcggct ggttgccatt gagcctcggt actgtgatgt
     1441 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1501 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1561 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1621 accttaaaca taacaaggta ttgcatgagc gggtgattct gttaactctg cgcaccgaag
     1681 acgcgccata tgtccataac gtccgtcggg tacagattga acaactgtcg cccactttct
     1741 ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1801 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1861 agtcgttgat cctcggcaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc
     1921 tgctgcaacg taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta
     1981 tcgaactggg tactcaggtc gaaatctaac gccagacgcc tcctttcttc ataagggggc
     2041 gtttttgttt tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta
     2101 acgtcacgat ggttttccca actcagtcag gattaagctg cggatcagcg aaacgtttcg
     2161 ctgatggaga aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc
     2221 tcccgtctgg gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa
     2281 agtacaacgc gtatcgatat ggcattaacc caaggtgtac cttcttttat gcaggtgctg
     2341 ggcgtcgtca caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa
     2401 cataatccgc aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag
     2461 ggaaatacca ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa
     2521 agtcaggcgg taattcgcag cggagaatgt tctccgtatg cgaatatcat tctctgtgct
     2581 ggcgtgacgt tctgaggccg tcatggaagc attacttcag cttaaaggca tcgataaagc
     2641 cttcccgggc gtaaaagccc tctcgggcgc agcgttaaat gtctatccgg gccgtgtgat
     2701 ggcgctggtg ggcgaaaacg gcgcgggtaa atccaccatg atgaaagtgc ttactggtat
     2761 ctatactcgc gatgccggca cgcttttatg gctggggaaa gaaacgacat ttaccgggcc
     2821 gaaatcttcc caggaagccg ggattgggat tatccatcag gaactgaacc tgatcccgca
     2881 gttgaccatt gccgaaaaca ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat
     2941 tgactggaaa a
//
LOCUS       DAJTSI010000272         2932 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000272 DAJTSI010000000
VERSION     DAJTSI010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.277"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            75..650
                     /gene="ssuE"
                     /locus_tag="OO327_004076"
     CDS             75..650
                     /gene="ssuE"
                     /locus_tag="OO327_004076"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCR6020401.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQTH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            643..1602
                     /gene="ssuA"
                     /locus_tag="OO327_004077"
     CDS             643..1602
                     /gene="ssuA"
                     /locus_tag="OO327_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR6020402.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1599..2744
                     /gene="ssuD"
                     /locus_tag="OO327_004078"
     CDS             1599..2744
                     /gene="ssuD"
                     /locus_tag="OO327_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HCR6020403.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2753..>2932
                     /locus_tag="OO327_004079"
     CDS             2753..>2932
                     /locus_tag="OO327_004079"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter"
                     /protein_id="HCR6020404.1"
                     /translation="MMATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPE
                     GVVTAFWTLSASGELW"
BASE COUNT          659 a          800 c          806 g          667 t
ORIGIN      
        1 tgtctctccg acagactgac cgaattgtta tcaatgttaa caaaaaaaag aacaattggt
       61 tataaggaga gagtatgcgt gtcatcaccc tggcgggtag tcctcgcttt ccttctcgct
      121 ccagctcctt gctggaatat gcgcgggaaa aactaaatgg cctagatgta gaggtttatc
      181 actggaatct gcaaaacttc gccccggaag atctgcttta tgctcgtttc gatagtccgg
      241 cactcaagac cttcaccgaa cagctgcaac aggctgatgg gctgattgtc gccacgcctg
      301 tgtataaagc cgcctattcc ggcgcgctga aaaccctgct cgacctgctg ccagagcgcg
      361 ctttgcaagg caaagtggtg ctaccgctgg cgacgggcgg taccgtggcc catctgctgg
      421 cggtcgatta tgcccttaaa ccagttttaa gcgcactgaa agctcaggag atcctgcacg
      481 gcgtgtttgc cgatgactca caattaattg attaccatca cagaccccag ttcacgccaa
      541 atctgcaaac ccatcttgat accgcgctag aaactttctg gcaggcattg caccgccgcg
      601 atgttcaggt tcctgacctt ctgtctctgc gaggtaatgc ccatgcgtaa catcattaaa
      661 ctggcgctgg cgggattgct tagcgtctct acttttgcgg ttgctgcaga atcctcgcct
      721 gaagcgttac gtataggcta tcagaaaggc agtattggta tggtgctggc aaaaagccac
      781 cagttactgg aaaaacgcta tccgcaatca aaaatctcct gggtggagtt ccccgcgggt
      841 ccgcaaatgt tggaagcgtt aaacgttggc agtattgatc tcggcagtac cggggatatt
      901 ccgccaatct ttgcccaggc tgccggggct gatttggtat atgtgggcgt cgagccaccg
      961 aagcccaaag ccgaagtgat tctggtggca gaaaacagcc cgatcaaaac cgtagccgat
     1021 cttaaaggtc acaaagttgc ctttcagaaa ggttccagtt cacacaacct tttactgcgt
     1081 gcactgcgtc aggccggact taaattcacc gatatccagc ccacttacct gacgcctgcc
     1141 gatgcccgcg ccgcgttcca gcaaggtaac gttgacgcct gggctatctg ggatccctac
     1201 tactccgctg cgttattaca gggcggcgtg cgggtgctga aagacggcac cgatctcaat
     1261 caaactggat cgttttatct ggcagctcgt ccctatgcag aaaaaaacgg cgcttttatt
     1321 cagggcgtac tggcaacctt tagtgaggcc gatgcgttaa cccgcagcca gcgcgaacaa
     1381 agcatcgctt tactggcaaa aacgatgggc ttaccggcac cggtgattgc ctcttactta
     1441 gatcatcgcc ctcctaccac catcaaaccg gttaacgccg aggttgccgc cttacagcag
     1501 caaacggcag atctgtttta tgaaaaccgt ctggtgccga aaaaagtcga tattcgccag
     1561 cgcatctggc agcccactca actggaagga aaacaattat gagtctgaat atgttctggt
     1621 ttttaccgac ccacggtgac gggcactatc tgggaacgga agaaggttca cgcccggttg
     1681 atcacggtta tctgcaacaa attgcgcaag cggcggatcg tctgggctat accggtgtgc
     1741 tgatcccaac ggggcgatcg tgtgaagatg cgtggctggt tgcagcgtcg atgatcccgg
     1801 tgacgcagcg gctgaagttt cttgtcgccc tgcgtcccag cgtaacctca cctactgttg
     1861 ccgcccgcca ggccgccacg cttgaccgtc tctcaaatgg tcgtgcgttg tttaatctgg
     1921 tcacaggcag cgatccacaa gagctggcag gcgacggcgt gttccttgat catagcgagc
     1981 gctacgaagc ctcggcggaa tttacccagg tctggcggcg cttattgctg ggggaaaccg
     2041 tcaatttcaa cggtaaacat attcatgtgc gcggagcaaa actgctcttc ccgccgattc
     2101 aacagcctta tcctccgctt tactttggcg gatcgtcaga tgtcgcccag gagctggcgg
     2161 cagaacaagt tgatctctac ctcacctggg gcgaaccgcc ggaacttgtt aaagagaaaa
     2221 tcgaacaagt gcgggcgaaa gctgccgcgt atgggcgcaa aattcgtttc ggtattcgtc
     2281 tgcatgtgat tgttcgtgaa actaacgacg aagcgtggca ggccgccgag cggttaatct
     2341 cgcatcttga tgatgagact atcgccaaag cacaagccgc attcgcccgg acggattccg
     2401 taggacaaca gcgaatggcg gcgctacata acggcaagcg cgacaatctg gagatcagcc
     2461 ccaatttatg ggcgggcgtt ggcttagtgc gcggcggtgc cggaacggcg ctggtgggcg
     2521 atggtcctac ggtcgccgcg cgaatcaacg aatacgccgc gcttggcatc gacagctttg
     2581 tgctttcggg ctacccgcat ctggaagaag cgtatcgggt tggcgagttg ctgttcccac
     2641 atctggatgt cgccattccg gaaattcccc agccgcagcc gctgaatccg caaggcgaag
     2701 cggtggcaaa tgattttatc ccccgtaaag tcgcgcaaag ctaaggaaca cgatgatggc
     2761 aacgccagtg aagaagtggt tattgcgcgt tgccccctgg tttttaccgg tgggcatcgt
     2821 ggcggtgtgg caactggcct cctcggttgg ctggctgtcg acgcgtattt tgccttcacc
     2881 ggaaggagtg gtgacggcgt tctggacgct ctccgccagc ggcgaactgt gg
//
LOCUS       DAJTSI010000273         2894 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000273 DAJTSI010000000
VERSION     DAJTSI010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.278"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..308)
                     /locus_tag="OO327_004080"
     CDS             complement(<1..308)
                     /locus_tag="OO327_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR6020405.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     "
     gene            complement(355..1074)
                     /gene="yehT"
                     /locus_tag="OO327_004081"
     CDS             complement(355..1074)
                     /gene="yehT"
                     /locus_tag="OO327_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR6020406.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1071..2756)
                     /gene="btsS"
                     /locus_tag="OO327_004082"
     CDS             complement(1071..2756)
                     /gene="btsS"
                     /locus_tag="OO327_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR6020407.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          748 a          774 c          657 g          715 t
ORIGIN      
        1 tcatcggttt tcagcgaaaa cgcgatgcgt aattttttca gcacgatgtt gttattaatg
       61 cgacgttccg gctccagtgc cggagcagac tcatccttgc cgcgtttttc ataaatcagg
      121 ccattgagga atgacgacaa aacaatgtcc ggacaacgct gaaaaccctc ttcgtcttct
      181 ttacgtagcc agacggcgat ctgttccgcg gtggcttcga cattatccag cgccagaata
      241 cgcaccaggt cattattatt ggctttcaaa atgtagcgca cgctgcgcag aatatcgtta
      301 cttatcatga ggccttcagg tgttgatgag gcaaaaagcc attttagcag tcttttacag
      361 gccaatcgcc tcttttaagc ttttcagata acggcggctg accggcacgg ttaagccatt
      421 acgcaaaatc aactcggcct ggccgttatc ttccagacga atctcctgta aatgcgcgag
      481 gttaaccaga tactgacgat ggcagcgcag tagtggtgta cgactttcca aggtacgtaa
      541 tgtcaattcg gtaaagccct ctttcccttc gtggctggta acgtagacac cgctcatccg
      601 actgctgaca aatgccacat ctttcatttg cagcaaataa atccgactat gccccgtaca
      661 agggataaat ttcagcgcct gttgattttc cggtaacagc gaaacatcct gcttgctgcg
      721 ctcctgacgc aatcgcgcca gcgttttctc cagtcgcgct tcatcaattg gcttcagcag
      781 ataatcaaag gcatgttctt caaaggcttt aatagcgtat tcgtcaaacg cagtgagaaa
      841 aacaatatac gggcgatgtt ccgggtcaag catccccacc atttccagac cactgatgcg
      901 cggcatctgg atatcgagaa acagcacatc cgggcgcagt ttatgcaccg cgccgatccc
      961 ttccacggcg tttgaacact ctccaacgat ttcaatatcg ctctgctcct gcaaaaatat
     1021 acgcaggttc tcccgtgcta acggttcatc atcgacaatt aagactttaa tcatgcctcg
     1081 tccctccatg gtagtcgtaa cgttattcgg gtgtaactat caggctcaca ggcgacgctt
     1141 attccatagt catcgccaaa ccgttcacgt aaacgcttat ccaccagatt catccccagc
     1201 ccactggcat tggttaccgg ttgatacaaa ccggcattgt cttcgatctc cagcatcaaa
     1261 tgttgcccct cacgtcgggc gctgattgcc actcgccctg tatccagcag ttgtgatgtc
     1321 ccatgtttaa tggcgttttc cactatcggt tgcagggtaa acgcgggcaa ttgctgctgg
     1381 gataattctt gcggaatagc aatgttgacc tgcaaccgcg actggaagcg cgccttttca
     1441 atttgcagat aagcgttcac atgttcaatt tcgtcggcga gagtaacaaa ctccgaaggc
     1501 cgctttaagt ttttgcggaa aaaagtggaa agatactgca ccagctggct ggcctgttcg
     1561 ctgtcgcggc ggatcaccgc tttaatggtg ttaagcgcat taaacaaaaa atgaggattc
     1621 acctgggcgt gaagcagttt gatttctgac tgggtgagca tcgctttttg ccgctcatat
     1681 tgccctgcaa gaatctgcgc cgaaagcagt tgcgcaatcc cctcgcccag cgtgcggttt
     1741 attgaactga ataaccggtt tttggcttca tacaatttga tggtgcccat cacgcgctga
     1801 ttttcgccac gcaacggaat taccagcgtc gaccccagtt tgcattgcgg atgcaaagag
     1861 caacgataag gtacttcgtt gccatcagcg tagaccactt caccggtttc aatcgctttt
     1921 aaggtgtaag tcgaagaaat cggtttgccg ggtaaatggt ggtcgtcacc aattccggta
     1981 aaggccagca atttctctcg atcggtaatc gcgactgcac caatatccag ctcctgatac
     2041 agtacctgag ccactttcat gctgttcact tcgttaaacc cctgtcgcaa aatgccttcc
     2101 gtcgaggctg ccactttcag cgcagtggca gaaaaagccg aagtgtattt ttcaaacatc
     2161 gcgcgtttat cgagcaatat acgcataaac agcgccgcgc cgacggtatt ggtgaccatc
     2221 attggcgcag caatattact caccagacgc accgcatctt cataaggtcg ggcgatcgca
     2281 aggatgatca gcatttgcac catttcagcg acgaacgtga cggcaccggc ggtaatgggg
     2341 ttaaagactt tatcagtgcg cccgcgacgg atcaggatgc tgtgtaccag gccgccgagc
     2401 aatccttcaa cgatggttga gatcatgcaa cttagcgcgg tcatgccccc catcgaatat
     2461 cgatgtaagc cgccggtcag accaaccagc ccaccgacga ccggaccgcc gagtaagccg
     2521 cccattaccg cgcctatcgc acgggtattg gcaatagaat cgtcaatgtg caacccaaac
     2581 caggtgccca tgatgcagaa gatggaaaag acgatgtagc agagaaattt atgcggcaga
     2641 cgaaccgtga cctgcattaa cggtatgaat aatggcgttt tactcattaa ccacgcaatg
     2701 actaaaaaaa cgcacatctg ctgaagcagc agcaacacca gattaaaatc gtacataccc
     2761 gcaaaccaca cttcccttta aaacgcgtaa catacattgc ctgcgtttaa ctttctttga
     2821 actcttgcag aaaaatgaga attcgtgagt acgatcactc aaaatcgcct ggcaaaaata
     2881 aaatcaccct atag
//
LOCUS       DAJTSI010000274         2888 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000274 DAJTSI010000000
VERSION     DAJTSI010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.279"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..779
                     /gene="lsrF"
                     /locus_tag="OO327_004083"
     CDS             <1..779
                     /gene="lsrF"
                     /locus_tag="OO327_004083"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HCR6020408.1"
                     /translation="WGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTIGLERIDINIAP
                     LFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLNS
                     CAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRI
                     AAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGVD
                     MGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            803..1093
                     /gene="lsrG"
                     /locus_tag="OO327_004084"
     CDS             803..1093
                     /gene="lsrG"
                     /locus_tag="OO327_004084"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCR6020409.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1133..1891
                     /gene="tam"
                     /locus_tag="OO327_004085"
     CDS             1133..1891
                     /gene="tam"
                     /locus_tag="OO327_004085"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCR6020410.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1895..2809)
                     /locus_tag="OO327_004086"
     CDS             complement(1895..2809)
                     /locus_tag="OO327_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HCR6020411.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          760 a          667 c          771 g          690 t
ORIGIN      
        1 attggggaat gcagtcacgc ttatcgtgga tatttaatcc gaaaacgggt aaaactgtga
       61 tgctggcctt tgaccatggt tattttcagg ggccgactat cggacttgaa cgcattgata
      121 taaatatcgc cccgctgttt gaacatgccg atgtattaat gtgtacgcgc ggcattttgc
      181 gcagcgtagt tcccccggcg accaataagc cggtggtact gcgggcgtca ggtgcgaact
      241 ctattctggc ggaattaagt aatgaagccg tggcgttatc gatggatgat gccgtgcgcc
      301 tgaacagttg cgcggtggcg gcgcaggttt atatcggcag cgaatatgaa catcagtcga
      361 tcaaaaatat tattcagctg gtcgatgccg gaatgaaagt gggaatgccg accatggccg
      421 tgactggcgt gggcaaagat atggtgcgcg atcagcgtta tttctcgctc gcgactcgaa
      481 tcgccgctga aatgggggcg caaattatca aaacctatta tgtcgaaaaa ggttttgaac
      541 ggattgttgc cggatgtccg gtacccattg ttattgctgg cggtaaaaaa ttaccggagc
      601 gcgagacgct ggaaatgtgc tggcaggcta tcgatcaggg cgcttctggt gtggatatgg
      661 ggcgtaatat tttccagtct gaccatccgg tggcgatgat gaaagccgta caggcggtgg
      721 ttcaccataa cgaaacggct gatcgggcat atgaactcta tctgagtgaa aaacagtaac
      781 tgcggattta aggagaagaa ttatgcacgt cacactggtt gaaattaacg ttcatgaaga
      841 caaggttgac gagtttatcg aagtttttcg ccagaaccac ctgggctctg tacaggaaga
      901 aggtaatttg cgcttcgatg tcttacagga cccggaagtg aattcgcgct tttatattta
      961 cgaagcctat aaagatgaag acacagtggc attccataaa accacgcctc actacaaaac
     1021 ctgtgtcgcg aaactggaat ctttaatgac cgggccgcgt aaaaaacgtc tgttcaatgg
     1081 gttgatgccg tgaggctaca attggggtaa cgcgctgacg ggagtaaaaa aaatgtctga
     1141 ctggaacccc tctttatatc tgcacttttc cgctgaacga tcgcgtccgg cggtggagct
     1201 gcttgccaga gtgccgctgg agaatgtcga atatgttgcc gatcttggct gtggcccagg
     1261 taacagcacc gccctgctgc atcaacgttg gcctgcggcc aggataacag gcatcgactc
     1321 gtctccggcg atgattgctg aagcgcgcag tgctttgcca gactgccagt tcgtggaagc
     1381 agacatccgc aactggcaac cggaacaggc actcgatctg atttttgcta atgcctcact
     1441 gcaatggctg cccgaccact acgaattgtt tcctcacttg gtttctttac ttaatccgca
     1501 gggcgtgctg gcagtacaga tgccagataa ctggctggag ccgacccatg tgctcatgcg
     1561 cgaagttgct tgggaacaaa actacccgga tcgcgggcgt gagccgttgg ctggcgttca
     1621 tgcttactac gatattttga gcgaagccgg atgtgaggtc gatatctggc gaaccactta
     1681 ctatcaccag atgccgtcgc accaggcgat tatcgattgg gtgaccgcca ctggattacg
     1741 tccgtggtta caggatctga ccgagagcga acagcagctt tttcttacgc gctaccatca
     1801 gatgctggaa gagcagtatc cactgcaaga gaacggacag atactgctgg catttccgcg
     1861 tctgtttatt gttgcccgcc gtacggagta attatcacgt cagctggtaa tgacgatcgg
     1921 gaagaatttt tgctggaatt tctgcttcat cgttcatctg taacaggtca atttcaatag
     1981 cgttgcagat ggcatccagc ggtaaatcat tgttttcagt accgaacgga tcttccagtt
     2041 cttccgccag acaatccagc gaaataaaag tgtaggaaat cagcacagag ataaaaggcg
     2101 tcatgtaatg caggtccacg accagcgcga acggcagcat gatacaaaac agataaacgg
     2161 tacgatgcaa aatcagggtg taggcaaagg gaattggcgt gtaggcaatg cgctcacagc
     2221 ctgccagaac cgctgaaata tcattaagcc gatcgttgag gctaataaac aggatatctg
     2281 aaagctgtcc attgcggcgc tgaaccgcca accattctcc cattattaac aagatacggt
     2341 tagctggaga gttcgaagcc agtacacgct gaagatctcc agtcttgaga taatgagcca
     2401 gcacttccgc ctgtggctgt ttgcgtaatg tcatgcgtaa acagtgggcg aaggcgattt
     2461 gcagcctggc aaactccctt acacttgccg aatccggcaa tgtcgttttt acctctcgca
     2521 gtaacgaccg tgaggcaatc atcaactgcc cccaaagttt tcgcgcttca acgtaacggg
     2581 cgtacccggc attattacga aaaccaagaa aaatggcgat ggcgacaccg agaatgctga
     2641 acggtgcgag ggtgaatttg atgcccagat gcgtgtacca gggcagcatg aatataacag
     2701 caatagaaaa gagaaaattg agtagtaagc gcgaggatat cttggataat actgagccgt
     2761 gccagacaaa aatacggcgc agccagtgtt gttgtggacg aacaatcatg gttatcttca
     2821 ggcgtggaaa agtcgcccta ttaaacgtga ttacgatcac attctcaaga cgctacttac
     2881 aaattacc
//
LOCUS       DAJTSI010000275         2810 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000275 DAJTSI010000000
VERSION     DAJTSI010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.280"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            73..834
                     /locus_tag="OO327_004087"
                     /pseudo
     CDS             73..834
                     /locus_tag="OO327_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(941..1441)
                     /locus_tag="OO327_004088"
     CDS             complement(941..1441)
                     /locus_tag="OO327_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020412.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
     gene            complement(1592..2740)
                     /locus_tag="OO327_004089"
     CDS             complement(1592..2740)
                     /locus_tag="OO327_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6020413.1"
                     /translation="MSITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQP
                     LGNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDR
                     KLSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAH
                     QSQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDG
                     TLALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTS
                     QLVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPIPFGAVISYSKDTENMAGIVGEDG
                     IAYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT          816 a          636 c          516 g          842 t
ORIGIN      
        1 gatgcaagaa tactcctaag taaatgaaag cgcaccacat gaaattatca acacatgtaa
       61 tttcatcatt aggtgctatc gaaaataact caggagctac ttaagatgaa taatgtctta
      121 ctcatgagac ccgatgactc agaatttatg atacctgatg actggtcagg gctaaacgca
      181 aaaggcatta catacgaaca agataataga gtgacagagt cttttctgcc ttttaaatct
      241 ttttattgtt caccggtgtg cacgacagtt gaactcatct cataaaataa ttgttttgct
      301 atgggtatcc gctaccttct ggaaaagaaa agagacttaa ggctaaatca ctttaaagcc
      361 ttctctcata aatgcgagac aaaacctgac gtgttaatta tgaccgggtg caccgtcgct
      421 gatacttata gtatattctc acgcattgtt gaagaaaata taatgaaaac gatgacacct
      481 atcgtcatca ttactgggca tcactcagaa gatctttgtt ctctggcatc gaaactggga
      541 ggagttaaaa taatatcacc gacagaaaag attgcaaaaa tagaaaacgc tatcaatgat
      601 gcttttaaca gcaagcctaa aaaaataaga tttttaacag atatactctc tcctcaacaa
      661 attgagacca tgaaaatggt tgcgcagggt agatccgtta atgagatttc agccgcaatg
      721 aatattagca tcaagaccgt gtatgcacat aaagttcgtg ctatcaataa gctgggaatt
      781 aagaaaaaag tttatgaagc gcttttttat aaagcgctaa ttcagttcaa ctgatgtaaa
      841 aatcaccctc gcttaataaa taatatattc tatgtctttt tttataagtt aaaaaaggcg
      901 ggatatcaaa tcccgccatg aaattaacta atcatctgca ttattctgcg tagatataag
      961 cctgggtacg gatggtgtaa gagtctggct caacaacctg ttctttcata tgggaaacga
     1021 cgcgataggc accacctgtt ccagtatttt tcagcatcat ataacgctta ccgccaatct
     1081 cttcggaggc agctgaagga ggatcctgct caagcgttgt cagggaaact ttcagtttat
     1141 ggtccgggtt attgtcacca gaaattactg catccgctga agtactgctc tccgcataaa
     1201 tatcatccgg gaaagttaca gcatagtcgt ggctctgccc atcagatgcg ccaacaatac
     1261 ctgttgccaa aggcgtccat gcagttaacg taccgctata tacccctttc actggttgaa
     1321 tggtattatt aacaacgata ggcttggtca aagtaagatt agctgacgct tcagtaccct
     1381 ggcttactaa agttcccgcc tgagaagcaa aagctgtccc cgccagcatg attgctaaca
     1441 atgttttctt cattatattt tctccatgat tttcatttac actgcaaaat gcagtttctt
     1501 tctttgcatt tacgtgctat cgcgaaaatg cttccaccaa ataatggaaa catcttcaca
     1561 aaaataaatt atgagtttct taatttagaa actataaaca tgtcgccgct atctggtata
     1621 accctgatgc acttttcttc gcaggaagtt ggtattttgc gatacaggat tgatctttag
     1681 aataacccca tttcacatta aactcgcctt ccgcactcaa gccagaaaca taagcaatac
     1741 catcttcacc tacaatgccg gccatatttt cagtgtcttt gctataacta atcacagcgc
     1801 cgaaggggat gggtttaccc tctttctgtt ttaaggttac taatccttta tccccgctct
     1861 tcactgccaa tgtcgcaggc acaacggctc cagctgtcgg tacaaccaat tgactagttt
     1921 gtggtaagtc gtaattctta cctatcgttt tttcatccag aataacatcg tctcttcggt
     1981 atggctgcac tggcagtatt gcatacccac gaaaatcagt actcagtccc ggccagccat
     2041 taacgttaac ccccgttgct ccgggtgcct ctataagggc taatgtccca tccacatttt
     2101 gaccaaatgt aagtccatcc cttgtaataa gtatcccacc gcttaaattt gcacctagct
     2161 gtttcatgtt ttcgctggag ctgtaatggc tactaaactg accatatgat ccatttttgc
     2221 caacttgcac aatgttcatc gtgctcttcg gttgtgactg atgagcgtta gttatatttg
     2281 tcgctacatc ccaccataca ccaccactat tctgacctga taatgaaacc tgatagttat
     2341 taccctgctc tcccgaacga ctcatctgtg aagacgcata aatttgattt tcgtcaccca
     2401 gcgataatgg cactgaaata cccacgctta atttccgatc tactttccat ttatgcgatg
     2461 tcagcaagtc gttttttgcc cagttaatgg agaatgttat gcctctccat accgtactat
     2521 aactcaggct ggtagaccgc gtgtttcctg tgccccggga agaattatac ctcgtggtat
     2581 tgaagttcaa attcccgata ttaccaaggg gctgggataa ggatatcgta gagccatcgt
     2641 gactattctt atctcttttt ctgttccgtt catcctgtgc acttactgct tcagtcagtg
     2701 tggcataacc tgatgcataa cggttcgtgg ttatagacaa tagactggcc ccctgaatct
     2761 ccagacaacc agtatcactt atttaagtga tagtcttaat actagttttt
//
LOCUS       DAJTSI010000276         2769 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000276 DAJTSI010000000
VERSION     DAJTSI010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.281"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..203
                     /locus_tag="OO327_004090"
     CDS             <1..203
                     /locus_tag="OO327_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020414.1"
                     /translation="GASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIAI
                     YLLAGCLISAMTALLMKDNQRS"
     gene            305..1759
                     /gene="amn"
                     /locus_tag="OO327_004091"
     CDS             305..1759
                     /gene="amn"
                     /locus_tag="OO327_004091"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR6020415.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2102..>2769
                     /locus_tag="OO327_004092"
     CDS             2102..>2769
                     /locus_tag="OO327_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6020416.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDAL"
BASE COUNT          760 a          615 c          621 g          773 t
ORIGIN      
        1 ttggtgccag ttatcgctac agtggtgctg gagtcggtta tcaggttgcc agtgtggttg
       61 gcggtggatt tacacctttt attgccgctg cactcatcac ttactttgcc gggaactggc
      121 atagcatcgc catttatttg ctggctggat gcctgatttc tgcaatgacc gctttattga
      181 tgaaagacaa tcaacgctcg tgatagcctg gcgaagatca tccgatcttc gccttatact
      241 tttgtttcac attcctgtga catactatcg gatgtgcggt aattgtatgg aacaggagac
      301 acacatgaat aataagggct ccggtctgac cccagctcag gcactggata aactcgacgc
      361 gctgtatgag caatctgtag ttgctttacg caacgccatt ggcaaatata ttacaattgg
      421 cgaattacct gatgaaaacg cccgtaaaca aggtcttttt gtctatccat cactgaccgt
      481 aacctgggac ggtagcacaa caaatccccc caaaacgcgc gcatttggtc gttttactca
      541 cgcaggcagc tacaccacca cgattactcg ccctactctc tttcgttcgt atctgaatga
      601 gcaactaacg ttgctttatc aggattatgg tgcgcatatc tctgtgcaac cctcgcaaca
      661 tgaaatccct tatccttatg tcatcgatgg ctctgaattg acacttgatc gctcaatgag
      721 cgctgattta actcgctact tcccgacaac agaactggcg caaatcggcg atgaaactgc
      781 agacggcatt tatcatccaa cggaattctc cccgctatcg cattttgatg cgcgccgcgt
      841 cgatttttcc ctcgcacggt tgcgccatta taccggtacg ccagtagaac attttcaacc
      901 gttcgtcttg tttaccaact acacacgtta tgtggatgaa ttcgttcgtt ggggatgcag
      961 ccagatcctc gatcctgata gtccctacat tgccctttct tgtgctggcg ggaactggat
     1021 cactgccgaa accgaagcgc cagaagaagc catttccgac cttgcatgga aaaaacacca
     1081 gatgccagca tggcatttaa ttaccgctga tggtcagggt attactctgg tgaatattgg
     1141 cgtgggaccg tccaatgcta aaaccatctg cgatcatctg gcagtgctac gcccggatat
     1201 ctggttgatg attggtcact gtggcggatt acgtgaaagc caggccattg gagattatgt
     1261 ccttgcacac gcttatttac gcgatgacca cgttcttgat gcggttctgc cgcccgatat
     1321 acctattccg agcattgctg aagtgcaacg tgcgctttat gacgctacca agttagtcag
     1381 cggcaggcct ggtgaggaag tcaaacagcg gctgcgtaca ggtactgtgg taaccacaga
     1441 tgacaggaac tgggagttac gttactcagc ttctgcactt cgttttaact taagccgggc
     1501 cgtagcaatt gatatggaaa gtgcaaccat tgccgcgcaa ggatatcgtt tccgcgtgcc
     1561 atacgggaca ctactgtgtg tttcagataa accgttacat ggtgagatta agcttcccgg
     1621 ccaggctaac cgtttttatg aaggcgctat ttccgaacac ctgcaaattg gcattcgggc
     1681 gatcgatttg ctgcgcgcag aaggcgaccg actgcattca cgtaaattac gaacctttaa
     1741 tgagccgccg ttccgataat aagttatgaa ccttcttatc agaaatagat gtgaaggatt
     1801 aagtaagaca tctggcaaat agcctgcaat aaacgcgggc tgcttgcttc cttgggcaga
     1861 tgcgagtttt attatcgtct taatgatttc cacatattaa aagcaagtat gctttcaaaa
     1921 cacaattata aaaaatcccg ccaacaacat tagtttttat aaaattaaat ataagattat
     1981 ggctttagaa tatttttatt tctaatagac gagatttttc ctgttatgat ataatatgcc
     2041 gaattaacac atgttaacga tttaccagta atgtaaataa attttcgagg agatcattcc
     2101 agtgggacgt aaatgggcca atattgttgc taaaaaaacg gctaaagacg gtgcaacgtc
     2161 taaaatttat gcaaaattcg gtgtagaaat ctatgctgct gccaaacaag gtgaacccga
     2221 tccagaatta aacacatctt taaaattcgt tattgaacgt gcaaagcagg cacaagttcc
     2281 aaagcacgtt attgataaag caattgataa agccaaaggc ggcggagatg aaacgttcgt
     2341 acagggacgt tatgaaggct ttggtcctaa tggctcaatg attattgctg agacattgac
     2401 ttcaaatgtt aaccgtacga ttgctaacgt tcgcacaatt ttcaataaaa aaggcggcaa
     2461 tatcggagcg gcaggttctg tcagctatat gtttgacaat acgggtgtga ttgtatttaa
     2521 aggaacagac cctgaccata tttttgaaat tttgcttgaa gctgaagttg atgttcgtga
     2581 tgtaactgaa gaagaaggta atattgttat ttatactgaa cctgctgacc ttcataaagg
     2641 aatcgcggcg ctaaaagcag ctggaatcac tgagttctca acaacagaat tagaaatgat
     2701 tgctcaatct gaagttgagc tttccccaga agatttagaa atctttgaag ggcttgttga
     2761 tgcccttga
//
LOCUS       DAJTSI010000277         2711 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000277 DAJTSI010000000
VERSION     DAJTSI010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.282"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            50..2572
                     /locus_tag="OO327_004093"
     CDS             50..2572
                     /locus_tag="OO327_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6020417.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEINHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIHQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAARLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSFSTPESESGLIEQGVTCH"
     gene            2583..>2711
                     /locus_tag="OO327_004094"
     CDS             2583..>2711
                     /locus_tag="OO327_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6020418.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFS"
BASE COUNT          726 a          585 c          677 g          723 t
ORIGIN      
        1 tgacccgata aaaatgacgc tgtaagcaag ggataaggat aggcgtatta tgatgacgac
       61 cagaatagtg gttggcctca cggcagggac gtgtctgatt ttctcgcaaa acctgatggc
      121 cgaggtcagt gtattcaatc cggcgcttct ggaaatcaac catcaatccg gagtcgatat
      181 tcgccagttt aatcgggcaa acctgatgcc cccaggtgtt tatagcgttg atatttttat
      241 caacggtaaa atgtttgaac gtcaggatgt gacatttgtt caggataatc cagatgctga
      301 tctgcacgct tgctttattg ccattaaaaa aacactgtcc tcctttggca taaaagttga
      361 tgcgctcaaa tcgttcaatg atgtggatga gacggtttgc ctcgatcctg ctccacgtat
      421 tgaaggctca tcctggcagt ttgacagtga taaattgcag ctgaatatat ccattcacca
      481 aatctacatg gacgcgatgg cttatgatta catcagcccc acgcgttggg atgaggggat
      541 taatgcgctc accatcaact acgatttttc tggttcacat acactacgtt cagattatgg
      601 ttcacaagag acagatacca gttatctcaa tctgcgcaat ggactgaata ttggaccgtg
      661 gcggctacgt aattacagta ctttaaacac cagcgatggc cgtgcggaat acaactccat
      721 tagtacctgg atacagcgcg atattgccgc gttaagaagc cagattatga ttggtgatac
      781 gtggacggcg agcgatattt tcgacagtac gcaaattcgc ggcgcgcgtt tgtatactga
      841 taacgatatg ctacccgcca gccagaatgg ctttgctcct gtggttcgtg ggattgcaaa
      901 gtccaacgcc accgtcatca ttcggcagaa tggctacgtg atttatcagt cagccgttcc
      961 acaaggtgct tttgagatca ccgatctcaa caccgcaagt acaggtggcg atttggacgt
     1021 aaccatcaaa gaagaagacg gtagcgaaca acgattcacc caaccttatg cttcattggc
     1081 gattcttaaa cgtgaaggtc tgacagatgt tgatgtcagc gtgggtgaat tgcgcgatga
     1141 agacggattt acaccggacg tccttcaggc gcaaatactt catggttttt cccacgggat
     1201 cactttatat ggaggtatgc aggctgctga aaattatggt tctgcagctc tgggtgtcgg
     1261 taaagatctt ggcgctttgg gcgcaatttc tttcgatgtg acacatgctc gtgcgaattt
     1321 tagccatgat gatacagaaa cgggtcagtc atatcgcttt ctctattcaa aactatttga
     1381 cgacacagac actagcttgc gcctggttgg ctatcgttac tccaccgagg gctactatac
     1441 cctcaatgag tgggcatcgc ggcgcaacag ccctgaagac ttttgggaaa caggtaaccg
     1501 acgtagtcgc gtggagggaa cgctaacgca gtcgttgggg agagattatg gcaatttata
     1561 cctgacatta agccggcaac aatactggca taccgatgat gtcgaacgat taatgcaatt
     1621 tggctacagc agtagctgga agcgtctctc gtggaacgtc tcctggagtt attccaatac
     1681 tgccagacag gggacgggga acaaccatgc cagtgataac accagtgagc agatctacat
     1741 gctctcttta tctgttcctt tatcgggctg gtggggtaat agttacgcca cctattctgt
     1801 ttcgcaaaac gataattccg gtagctcaca tcaactcgga ctcagcggta cggcgctgga
     1861 aagaaataac ctttcatgga atttaatgca gtcctataac agtcatgatg atgaggttgg
     1921 cggtaatatg tccctgacct atgatggctc ttatggcacg gtgaacggca gctataacta
     1981 cagccaaaat tcccagaggc tgaattatgg tatcagaggg ggaattctgg cacacagcga
     2041 aggggtaacg ttaagtcagg agttaggtga aactattgct cttgttaaag cacctggggc
     2101 cgccaggtta gaaatagata atatgcgcgg tgctgcgacg gactggcgcg gctatacggt
     2161 caagacacag ctaaaccctt atgatgaaaa tcgggtagca atcagcgata actatttctc
     2221 gaagtcgaat atagaacttg ataataccgt cgttacgatg gttcccacgc gtggtgcagt
     2281 ggttaaagcg gagtttgtga ctcatgtggg ttatcgcgtt ctcttcagag tgttaaatgc
     2341 aaatggtaaa ccggtacctt ttggagccat tgctgcgata caagatgcaa gtttggcaga
     2401 ttcaggaatt gtcggtgacc gtggcgaact ttatctttct ggtctaccag aaaaaggaca
     2461 ggttacgtta tcctggggag aaaacgcctc aacaaaatgc atcttcaatt attcattttc
     2521 gacaccagaa agtgagagcg gattaattga acagggtgtg acatgtcatt aaaaggattt
     2581 ttatgaacaa gtatatcaaa cagtggtgct ttgctgtgtt tatgctctcg ttaagtagtg
     2641 tagcacttgc agctcctaaa ggtatctgta cccctgataa tggcgtattt cacagtacgc
     2701 ttgatttctc g
//
LOCUS       DAJTSI010000278         2628 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000278 DAJTSI010000000
VERSION     DAJTSI010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.284"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..545)
                     /locus_tag="OO327_004095"
     CDS             complement(<1..545)
                     /locus_tag="OO327_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020419.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDETQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDG"
     gene            complement(1207..2196)
                     /locus_tag="OO327_004096"
     CDS             complement(1207..2196)
                     /locus_tag="OO327_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020420.1"
                     /translation="MNMTKNAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
     gene            complement(2280..>2628)
                     /locus_tag="OO327_004097"
     CDS             complement(2280..>2628)
                     /locus_tag="OO327_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020421.1"
                     /translation="TSREAMELLEALAEAGVTIGNFFSNDLMTAYQRRQMNKRAEAER
                     IAKELASQKERTREMFMTEDEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIV
                     ADLESGGNWMDSL"
BASE COUNT          682 a          613 c          632 g          701 t
ORIGIN      
        1 attccatcca gtcgttttgg gttgctaatg atgtcctgca tccgcttaat ctggtcgggc
       61 gtagacatat aaccccacag ggtgagcaga accttttttg cggctacact atttccttgg
      121 tctgatacta atgccatata gccccataaa ctatccctca gccagacagc atcatagtgg
      181 gtttctgccg ctgttttgtc atcttcgaaa gtagaagcaa gaataaacgt tccccagggt
      241 ttctccgtca acgttaactt tcgtgtgttt tctgttaatg ttagcaatcc ttgagtttcg
      301 tctttggtat agtgactctt tatttcattt tttaatttgg ctgagtgaat cagaagacga
      361 taattaggat acttatctgg atttaacgat aaataacttt tatctgttgc tgcccccctg
      421 gaattaagaa tgttaaatac agattcgctt gtcacttctg cctctgtcgc ctggcatgtg
      481 ccgcacgaaa atataccagc gagcagaaga gttaatgcag atttcccttt cataaaataa
      541 tccatcaatc gtttgttaaa agcgtagata accacttatt caacaacttg aaaataaggt
      601 attttacctg agttgttaca aaaggattgc attgcgtaaa cgctttttat ttacaaaaaa
      661 atggggaagt gttacagcga ttgcgaaaag cctgaagtga ttgaattaag ataatataga
      721 aaatcagata cagcaaaaat ggcagattga tcaggtgaag ataaattgga gcgggcgaag
      781 ggagcctacg acaaatcgaa gacaaccagc taacctattg tttcttaaag acaggtgaat
      841 ccagttggtt gtagtgatag tcacacattt agagatagaa ggtcaagact tgaagcacgt
      901 aatattccct ctaaaggaat agaactcacg gtctgactac ccggcccttt tggtctggct
      961 caggtcaggc tatgggggct tttggctcgc tctttatatt agatgaggtc tcacattttt
     1021 cagtcaaaac tcatcgtcta atagcccata gccagaccac cagtcccagc tttagcagga
     1081 agaccacgag atagactttg tgtaggtcca tttggccctc ctactttctg tattcacaaa
     1141 ctagattact agtacaaaat aaaggccaga catccagttc ggaagtctga ccaatagtcg
     1201 ttattgttag ttagcttctt tagtgaagct caggcgtttg actacaggca ggacagcagt
     1261 agccgagcca gtacccatct tgatggtcgt agcagcttca gctacaacaa acaccttgcc
     1321 gagaatctca aggtcgtgag actgggagtc tccatcacgt gaaaggacca cagtaatgct
     1381 ctcgccatcg tgacggaagc aaccgaagct cagagccgta aaacgcttag ccatcatgaa
     1441 cacgccacgg tcttcaccgt tgtaataggc gacgtcagtc cccagcatat cgacgatagc
     1501 gtcttcacca ccgaagccct gcttagcagc cagacgggag agagccttga ggtgtgactc
     1561 atgggcgata atgccgtagt cagaaacctc agaaccgaaa gaagactccg tgttctcgta
     1621 cagggcatcc atgatagcgt cagccttagc ggtcaggcta ccagtcaggg ccggaagttc
     1681 tacggtcttc agcagctttg aggactccag cacgtcaacc atcttagagt taatggtacg
     1741 aatcacgtta tcgcagatgt tcagtgcgtc ctgctggagc gtcccctgag tgaaaccagt
     1801 ggtcgacttc tggtcccaaa gctttgtttg gtgagacacg aggatcgcct gttcaatcgg
     1861 ctcaacggtg atttcagtgc cagacggagc ggtcacaact tcaggtcgct taccaccacg
     1921 gacagggacc agcacagagc ggtcagccag agtgtgagca atgattggag tcggtaagct
     1981 gttacgggtc ggcaggactt ctacagggaa cgggatgtta tcttgagcgt ctttaagtac
     2041 accgttcagc atagccttaa cagtcttagc taactggagg gcatagacct gagggtcggc
     2101 gttagtcggc agaccttcat tgacctgaat gttgtcggta tcggtgatgc gtactggctc
     2161 aatgccaaac tggaaagcgt ttttagtcat attcattata attaatctcc ttgagtcaaa
     2221 tacttgaagt ccctaacagg cagtcaggga ccgtgtgttc tcagatgagg gacgcttgct
     2281 tacaagctgt ccatccagtt accgcctgac tcaaggtcag cgacgatagc ggcacgtgag
     2341 cgaccagcgg aatgagtagc gctgcggagg ttttcaccat aggtccggcg ttcagctttg
     2401 acctgttcgc ggcgctgtag ctctttttgc cattcgtctt cggtcatgaa catttcgcgg
     2461 gtacgctctt tctgagacgc tagctcttta gcgatacgct cagcttcagc tcgtttgttc
     2521 atctggcggc gttggtaggc ggtcatcagg tcattactga agaagttacc aatggtcacg
     2581 ccagcttcag ccagagcttc cagtaactcc atcgcctcgc ggctggtg
//
LOCUS       DAJTSI010000279         2610 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000279 DAJTSI010000000
VERSION     DAJTSI010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.285"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..672)
                     /locus_tag="OO327_004098"
     CDS             complement(<1..672)
                     /locus_tag="OO327_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR6020422.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPL"
     gene            complement(660..1043)
                     /locus_tag="OO327_004099"
     CDS             complement(660..1043)
                     /locus_tag="OO327_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR6020423.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(1059..1367)
                     /locus_tag="OO327_004100"
     CDS             complement(1059..1367)
                     /locus_tag="OO327_004100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020424.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(1368..1946)
                     /locus_tag="OO327_004101"
     CDS             complement(1368..1946)
                     /locus_tag="OO327_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR6020425.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(1969..2400)
                     /locus_tag="OO327_004102"
                     /pseudo
     CDS             complement(1969..2400)
                     /locus_tag="OO327_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT          662 a          686 c          732 g          530 t
ORIGIN      
        1 tagtggctgg agcgctccgt tttgcaattg ccacgcttgc tggcagtagt tcaggaaagg
       61 tgtgcgatga cgttcaaaaa gaataatcag cgaaccacgt tcaaacgccc agtgttgtaa
      121 gcgctgcaac agcatttcgc tggcctgcgg tgtcaggcgg ctaaaggctt catcaagaat
      181 taagagcttt ggctgcatcg cgatggcgca ggcgatgatc acgcgctgtg tctctccacc
      241 ggagagtgtt gccgggtgac gatggcgcaa cggctggcac tcggtcagag tcagggcggc
      301 atcaatccgc gccataatct ctgcttcagc aagacacaga ttttctggcc caaaggcaac
      361 ttcttcttcc acgctaaagg tacagccgga gagctgcaaa tagggcgatt gctggacgag
      421 ctggatggtg gcggattgtt cattaagcgg gagatgccca atgggcgtac cgaggagcgt
      481 ccccgtgcct gttatctctc ttggcagaaa gtccggatac cagcccgcca tcagctgtgc
      541 cagcgtactt ttgccactgc cgttgtcacc aaagatggcg accatccccg gtttgacata
      601 gtgaaagtca taacagaacg gaggatgcgt tgagtgtgtc gggcaataac ggaactgttc
      661 taacgtaacc atatccacgc tccgaattct gtcagcatca gcaagatcat ggtgtaacgc
      721 gctacgcgtt gtagggtgct atcggctggt gcccacaacg ttgttcgtcg gttgtggatc
      781 cgaaatgccc gcatatccag cgccgcacca cggacagcca gatcgttaag cgcattgtgt
      841 gttagcggaa taattaacgc gggcatagca cgtaaacgct gataccagcc ctcatccagc
      901 ggcacgccgc gggcacgctg cgcttcatga ataatcgcta attgccgttt taactgctca
      961 acgaccaata acggcccggc aaacagataa gcgacgcctg gcggcaggcg agaagcaaac
     1021 agagcgcgaa taaaacattg cacggggacg tactgcattc acaactgtga ggttgaaaca
     1081 atcgccagaa tcagcaacca gagcgtaata gcatgcgacc accgttctgg cgagcgtggc
     1141 gtgccgctta gccattctgg tagccagcca ccgtgtacca gccacagccc ggctcccagc
     1201 gagaacatca gccagacgac atattttgcc cgccgccgcg tggcctttag cgcaataagg
     1261 cagaaaaagg tcgccgcgct atagatcggc aaaatggtct gcgcgggcag gataagcgtg
     1321 gtgcaggccg ctaatgccca cagtgttaat gaggtaaatg gatgcattta gcgcacggca
     1381 gccattgccg gaaaatggcg cgtggtgcga atcggcagct ggcgcagtaa aagccagaca
     1441 atcaccgctg taagaatttt atcgaccagg ttcgcgccta tcaccgtaat cgccaccgac
     1501 tccaccaggt tttgccccat cgagtgcatc caggcaacga acaaatcggc tccgctaccg
     1561 gtgacaccgc cgaacagcgc agtacgcagc ggtacagcga cgaccgttac cgcaagcgta
     1621 ataatcacgc cgctgacaac gacttttggc agggtgcgaa accaacccgc tcgcgccagc
     1681 caaccggaga ccaggccgat gaccatcgcg acgggtgcaa atgcggcggc gatcgggtcg
     1741 gtcagcaatc cccagagtaa attggtcagt aagccggtca gcattccgat caccggacca
     1801 agcagtaccg cgctaataag tgtgccgatg gagtcgagaa atataggtaa tttgaccatg
     1861 ctggccagtt gcccgccgat catattgatg gcgatagata taacgatcaa caccagtgcc
     1921 tgactggaaa agtggctacg cgccataaga aaacctcttt gaaagtagtt atttaacggg
     1981 ggaaatatgc aactcgtcgt aaccagaaag cgcgcaaggt tggcggctaa acgccagttc
     2041 ctcaaggcta ccgatcacca gttcaagcgt ggtccgcacg gtacagccgg gcatcatatg
     2101 accgccaagc atcgtgccgt gtggatcgga aacgcagaga tgaagatgct caccactctg
     2161 ttccagcgtg ccgtttagtg agatcacttc gaatgtaccg ttcaggagcg tggtgccttc
     2221 ttgcccggca tagcgcaagg cgacgtcagt caggctaccg gtacagcctg caatctaggc
     2281 ggcatgaagt tgatgttgct gtgcaaaagc gtgtagctgg gaaaaaactt cctgcccggg
     2341 gaggaggcgc agtgcgtaaa agcgtgcgga ggaagaatat gagtagggca atgtcatcat
     2401 tggagtactc cgaaactcgg acgtattgcg gataatatcg acgctgcatc agtcatattc
     2461 tgatataagt cagggtttgt gacggcacaa cgaaaagatt ttgctacgct tattcgtgat
     2521 tctgcaaagt ggaataaatt ccacaggggc ggggctgaaa acgtgacgta agtcagataa
     2581 ttgctttctt tgactggaca atcattcctt
//
LOCUS       DAJTSI010000280         2572 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000280 DAJTSI010000000
VERSION     DAJTSI010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.286"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(64..822)
                     /locus_tag="OO327_004103"
     CDS             complement(64..822)
                     /locus_tag="OO327_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR6020426.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="OO327_004104"
     CDS             complement(830..1933)
                     /locus_tag="OO327_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR6020427.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..>2572)
                     /locus_tag="OO327_004105"
     CDS             complement(1943..>2572)
                     /locus_tag="OO327_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR6020428.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAVIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          711 a          683 c          601 g          577 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatgctg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagtccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagca
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat aacggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc attccgccgc ggaagttaaa gccttgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggag cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2521 agcggaatag cccaacagaa aggactatag ccataacaac agccaggata aa
//
LOCUS       DAJTSI010000281         2569 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000281 DAJTSI010000000
VERSION     DAJTSI010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.287"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..289
                     /locus_tag="OO327_004106"
     CDS             <1..289
                     /locus_tag="OO327_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6020429.1"
                     /translation="YDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQE
                     MGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNSSQSKNKEGTNDV"
     gene            638..1810
                     /locus_tag="OO327_004107"
     CDS             638..1810
                     /locus_tag="OO327_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6020430.1"
                     /translation="MHMIEINSLLLITSVILMSLLAVGLFDKISPINLVEHGRNNQID
                     GMRGFLAIFVLIHHAAIWNGYLSSGVWEAPSSNLLANLGQVGVSFFFMITGYLFFSKI
                     ISGDQDWTRLYVSRLLRLTPMFIVSLCLIFIIVGFKSGWRMQVSTEELFVSIMKWLPF
                     TALGMPNINDVKDSFTINAAVTWTLVYEWFFYFSLPVISALIKRKVSIYMVMISAISL
                     FVFILFFSKIHIASFLFGLLAFLLNKSKIVNGIAKAKVTPVIITAIMVFEMTYFKTTY
                     APLPLILCGITFIIIASGCDLYGILRLNITRKLGETTYSVYLLHGIFLYCLMTWIIPN
                     NYTENTFIILVSTTAFLITFTSCLTFKLIETPFIKLTKQTTTLVKELIPTLTNNNQ"
     gene            complement(1864..2487)
                     /locus_tag="OO327_004108"
     CDS             complement(1864..2487)
                     /locus_tag="OO327_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6020431.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSARY
                     THTNIQVTSSGGGKVTVILNGTTFQSAGDYQPSAERPYWITGANCEVVDIGCVFTETT
                     SKVTSASALSVTRSGKINANGSIDVATGVSSVNVVSTGVYDVSFSHPLAAVASGYIVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLI"
BASE COUNT          787 a          533 c          483 g          766 t
ORIGIN      
        1 gtatgacgca aacacagcct ggaaagcggc gctgagacga gcaggtattg atgatttcag
       61 atttcacgac ttgagacaca cctgggcaag ttggctggtt caagccggag tcccgttgtc
      121 agtgttacag gaaatgggag gctgggagtc tatcgaaatg gttcgtcgat atgctcacct
      181 tgcacctaat caccttaccg aacacgcacg gcaaatagac tcgatcctga acccatcggt
      241 cccaaattcg tcccagtcaa aaaataagga aggtactaat gatgtgtaac ttattgattt
      301 aaatggtgcc gataatagga gtcgaaccta cgaccttcgc attatgaatc tgtagcacca
      361 atcataacta tctgttttag caagcattaa ccgcattcac taagctatag ttgatgacac
      421 aaacagaaag ttgatgcatg atgttgccat atatatgtca caaatacggc acaacgatct
      481 tcaaacatat agccacacat tcacagaaga gcacaaagcc ttgcaatcca gtgcaaagct
      541 tggtgtgtct ccgttttgtt tcatcagtta cacataacta tatgatcatt aactaaaagt
      601 cagtatactt ggagaaattt taattgcgta atagagcatg catatgattg aaattaacag
      661 tttattatta ataacatccg tgatattgat gtcgttatta gctgtggggt tatttgataa
      721 aatttcccca ataaatcttg ttgagcacgg caggaataat caaatagatg gaatgcgggg
      781 gttcttagca attttcgtgc ttattcatca cgcagcaatt tggaatggat acttgtcatc
      841 tggagtatgg gaagcacctt catcaaatct gttagcaaat ttaggccaag ttggtgtgtc
      901 attctttttt atgattactg gttatctgtt cttttcaaag attatctctg gagatcagga
      961 ctggacaagg ctttatgtat caagattact acgattaacc ccaatgttca tagttagcct
     1021 atgtctcatt tttatcattg taggttttaa gtctggatgg agaatgcagg tatccacaga
     1081 agagcttttt gtgtcaataa tgaagtggtt gccattcact gcgctaggta tgccgaacat
     1141 taatgacgta aaagattcat ttactatcaa tgccgctgta acatggacac ttgtatatga
     1201 atggttcttt tatttttctc ttccggtaat ttccgcgctc ataaaaagaa aagtcagtat
     1261 ttacatggtt atgattagcg caatatcgct atttgttttc attttatttt tcagtaaaat
     1321 acacatagca tcatttcttt ttgggctcct ggcatttttg ctaaataaat caaagatagt
     1381 caatggaatt gccaaagcaa aagtaacacc agttataata actgcgataa tggtttttga
     1441 aatgacgtat ttcaaaacaa cttacgcacc gctaccactg attctttgtg gaataacatt
     1501 tatcattatc gcatcaggtt gtgatttgta tggaatatta agattaaata taaccagaaa
     1561 gctaggagaa actacctaca gcgtttacct tctgcatggg atattccttt attgcctaat
     1621 gacgtggatt attcctaata attacactga aaatacattt atcatactgg taagcacaac
     1681 tgcatttctt attacgttca catcatgcct aacttttaaa ttaatagaga cgccattcat
     1741 caaattaaca aaacaaacca caactttagt aaaagaatta atacccacat taacaaacaa
     1801 caatcaataa acaataaaca taagccgccc tacatatatg tccaaatggg gcggcgaatg
     1861 tatttatatt agtctggtaa tgctaaatgc aaacgaactg gagattcctg ccccgctaag
     1921 cgtattgcgc agggtcaccc taaatgtatc tacgccaata taagtaacat cacaactaac
     1981 tgaatcaggc gcagatatag gtgttatctg cacaatataa ccgctagcaa ctgctgcgag
     2041 cggatgagaa aagctaacat catacacacc tgtagaaacc acgttaaccg atgaaacacc
     2101 agtagcaacg tcgatactac cattggcgtt aatttttccc gagcgtgtga ctgacaatgc
     2161 agaagccgat gttaccttac tggttgtttc tgtaaaaaca cacccaatgt caactacctc
     2221 gcaattagct cctgttatcc agtatggtcg ttctgcactt ggctgataat cccctgcgct
     2281 ctggaacgtt gtgccgttga gtatgactgt aacttttccc ccacctgagc ttgttacttg
     2341 aatattcgtg tgcgtgtatc ttgcactgct cacgcgatga aaatttccac cgtttataac
     2401 cacagttaca gggcgcgtcc cagtattatc tatagcaagg tctgcccccc cggcattagc
     2461 ctcaaagtat gggttgttta ataccaaagc gcacgaaccg ttaaggccat ttatagctaa
     2521 ttgcgcacca cctgtactgg aatccccctg agtaccattt ccttcacaa
//
LOCUS       DAJTSI010000282         2528 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000282 DAJTSI010000000
VERSION     DAJTSI010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.288"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..258)
                     /locus_tag="OO327_004109"
     CDS             complement(<1..258)
                     /locus_tag="OO327_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020432.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMS"
     gene            283..522
                     /gene="relB"
                     /locus_tag="OO327_004110"
     CDS             283..522
                     /gene="relB"
                     /locus_tag="OO327_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HCR6020433.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            522..809
                     /gene="relE"
                     /locus_tag="OO327_004111"
     CDS             522..809
                     /gene="relE"
                     /locus_tag="OO327_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HCR6020434.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            881..1036
                     /gene="hokD"
                     /locus_tag="OO327_004112"
     CDS             881..1036
                     /gene="hokD"
                     /locus_tag="OO327_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR6020435.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1254..1505
                     /locus_tag="OO327_004113"
     CDS             1254..1505
                     /locus_tag="OO327_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020436.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            1572..1850
                     /locus_tag="OO327_004114"
                     /pseudo
     CDS             1572..1850
                     /locus_tag="OO327_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1852..>2528
                     /locus_tag="OO327_004115"
     CDS             1852..>2528
                     /locus_tag="OO327_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6020437.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLP"
BASE COUNT          654 a          595 c          662 g          617 t
ORIGIN      
        1 tgacatgcta aagcaaaaaa tagatgaata agttgagttg tgcatatgta gcctgaccgt
       61 cacaaagtat atggtgtctg taccagtaag atgatggccg gactctttaa aaaagagctg
      121 acctgcacaa tacaggatgg acttagcaat ggctgctcct ggcacaaagc ggacagtgat
      181 caccgttctt acgactactt tctgacttcc ttcgtgactt gccctaagca tgttgtagtg
      241 cgatacttgt aatgacattt gtaattacaa gaggtgtaag acatgggtag cattaacctg
      301 cgtattgacg atgaacttaa agcgcgttct tacgccgcgc ttgaaaaaat gggtgtaact
      361 ccttctgaag cgcttcgtct catgctcgag tatatcgctg acaatgaacg cttgccgttc
      421 aaacagacac tcctgagtga tgaagatgct gaacttgtgg agatagtgaa agaacggctt
      481 cgtaatccta agccagtacg tgtgacgctg gatgaactct gatggcgtat tttctggatt
      541 ttgacgagcg ggcactaaag gaatggcgaa agctgggctc gacggtacgt gaacagttga
      601 aaaagaagct ggttgaagta cttgagtcac cccggattga agcaaacaag ctccgtggta
      661 tgcctgattg ttacaagatt aagctccggt cttcaggcta tcgccttgta taccaggtta
      721 tagacgagaa agttgtcgtt ttcgtgattt ctgttgggaa aagagaacgc tcggaagtat
      781 atagcgaggc ggtcaaacgc attctctgaa ccaaagcatg acatctctgt ttcgcaccga
      841 aggtgacact tctgctttgc gttgacagga gaagcaggct atgaagcagc aaaaggcgat
      901 gttaatcgcc ctgatcgtca tctgtttaac cgtcatagtg acggcactgg taacgaggaa
      961 agacctctgc gaggtacgaa tccgaaccgg ccagacggag gtcgctgtct tcacagctta
     1021 cgaacctgag gagtaagaga cccggcgagt gagaaatccc tcgccacctc tgatgtggca
     1081 ggcatcctca acgcacccgc acttaacccg cttcggcggt tttttgtttt tattttcaac
     1141 gcatttgaag ttctggatgg tgccggaata gaatcaaaaa tacttaagta gcgcgcaggg
     1201 ataaaaggga tggaccccga acaggggagt gctacttatc tggaaggatt ctgatgatga
     1261 acatcgaaga actgcgtaaa attttttgtg aagatggcct ctatgctgtg tgcgttgaaa
     1321 atggaaatat tgttagtcat taccgcattg tgtgtttgca aaaaaatggg gctgcgttaa
     1381 ttaattttgt ggatgcccga gtgacggacg gatttatctt gcgcgacggt gagtttgtca
     1441 cttcattaca ggcattgaaa gagatcggaa taaaagctgg cttttctgct ttttcagaag
     1501 aataaactca tctacaatct tgcgcggggc tgaactcccg ctgagtaaca ccgtgccacc
     1561 ggagaaaacc gatggcacgc aacgcaaaat attacaatca tgataattcg accgttcttg
     1621 cccacacgca cgagcggtat tctcacgcat ttaagtcaga ctggtaccag catccctcat
     1681 gcactgaaga acaggccgaa tggcttattc agtgttaccg caggcgcgga tgcgaggtta
     1741 aaaaagccct tagcctcgac taccgtcact agataatctc cgtcaggctc ccttactccg
     1801 aacagccacc gcgtccgtcc cgcacattcc agcaacggat ctggaggtaa cgtgcgggta
     1861 ttacttcgac ctgttctggt tccggaactc gggctggtgg tccttaagcc cggtcgtgaa
     1921 tccatgccgg tattccacaa tacccgggta ctggtggagc cggaaccgaa aagcatgcgt
     1981 aatctgccgt ccggggtcgt tcctgccgtt cgccagccgc tagtggaaga caaaacattg
     2041 ctgccgtttt tcagtaacgc acgggtgatt cgtgctgctg gtggtgctgg cgcattgtct
     2101 gactggctgt tgcgccatat taaatcctgc cagtggccac acggcgatta tcatcacagc
     2161 gaaaccgtca ttcaccgtta tggtaccggc gcaatggtat tgtgctggca ctgcgacaac
     2221 cagctgcgtg accagacatc cgaatcactc gagcaacttg ctcatcaaaa cctgtcagca
     2281 tggatgattg acgtcatcgg tcacgcaata agcggtacgc aggagcgtga attatctctg
     2341 gctgaattat cctggtgggc ggtccgcaat caggtggcgg acgcgctacc ggaagcggta
     2401 ttacgtcgtt cgctggggtt gcgtgcggaa aaaatccgct caatgtaccg tgaaagcgac
     2461 atcgtgccgg gagagcagac cgccaccagc atactgaagc agcgcacaaa aaatcttgcg
     2521 ccgctgcc
//
LOCUS       DAJTSI010000283         2516 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000283 DAJTSI010000000
VERSION     DAJTSI010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.289"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..91)
                     /locus_tag="OO327_004116"
     CDS             complement(<1..91)
                     /locus_tag="OO327_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal protein"
                     /protein_id="HCR6020438.1"
                     /translation="MKKKQFLKESDVTAESVFFMKRRQVLKALG"
     gene            complement(200..613)
                     /gene="hiuH"
                     /locus_tag="OO327_004117"
     CDS             complement(200..613)
                     /gene="hiuH"
                     /locus_tag="OO327_004117"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR6020439.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            746..1417
                     /gene="hprR"
                     /locus_tag="OO327_004118"
     CDS             746..1417
                     /gene="hprR"
                     /locus_tag="OO327_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR6020440.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            1417..>2516
                     /gene="hprS"
                     /locus_tag="OO327_004119"
     CDS             1417..>2516
                     /gene="hprS"
                     /locus_tag="OO327_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR6020441.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPE"
BASE COUNT          745 a          470 c          556 g          745 t
ORIGIN      
        1 tgcccagtgc ttttaacacc tgccgacgct tcataaagaa taccgactcg gccgtaacat
       61 ctgattcttt taaaaattgc ttctttttca taagccatca cacttttttg tcgttaacca
      121 acaagagctg tttactgtta atctgtcagg gagtcacctc cctaacagta gcagagatca
      181 gcttttacat atttacgcat taactgccac gataggttga atacccatat tggctgagta
      241 acaaaggcac atgataatgc tcgttcactt tattaatatg aaattcaacg ggaatctcag
      301 ggaagaaact ttcaagattt tgtttcttga aatagtcccc ggttttaaat acgacacggt
      361 aatcgccagt agtcgcagtt tgctcgggcc acagtgcctt aattcgtcca tccttatctg
      421 ttttggcggt attaagttgt aaccagccgt tatccgcttt cttttcaaga gtgactgtca
      481 cgtcggcagc gggttttcct gtttgctggt tcaaaatgtg cacgctaaga atgttttgtt
      541 gtgcggcata aaccaaagaa ggtaatgaaa aggctgccgt tgctaccgag agtactaaat
      601 aacgctttaa catgtttata tccttgtcat gtgaatgagt tttgaggata gcgaaaaaac
      661 gtccagggta accttacagc agcattacaa ttttgtaatg aaccaggttg tttctataac
      721 atatgattta tggcatatta ttttcatgaa gattctactt attgaagata atcaaaggac
      781 ccaggaatgg gtaacgcagg ggctttccga agcgggttat gtcattgatg ccgtttctga
      841 tggcagagat gggctttatc ttgcgctgaa ggatgattat gcattgatca ttctggatat
      901 tatgcttccg ggtatggatg gctggcagat cttacaaacg ttaagaacgg caaagcaaac
      961 ccctgttatt tgccttactg caagggattc tgtcgatgac agagtcagag ggttggacag
     1021 tggggcaaat gattatctgg taaaaccttt ttcattttct gagttgctag caagggttcg
     1081 ggcacaatta aggcaacatc acgctctgaa ttcaacatta gaaatcagtg gcttaaaaat
     1141 ggactctgtc agtcaaagtg tcagcaggga caatatcagt attacactga cgcgcaagga
     1201 gtttcagtta ctttggctac tggcctccag agctggcgag attataccca gaacggttat
     1261 tgcgagtgaa atttggggaa tcaactttga tagtgatacc aatacggtgg atgtcgccat
     1321 tcgcaggctc cgcgcaaaag ttgatgatcc ttttcctgaa aagctaatcg ccacaatccg
     1381 ggggatgggc tattcattcg tagcggtaaa aaaataatga aaagattatc tataaccgtc
     1441 cgtttaacct tgctttttat atttctgcag tctgttgctg gcgctggaat tgtatggact
     1501 ctctataatg gcctggcaag tgagttgaaa tggcgcgatg atacaacact cattaaccgg
     1561 acagcgcaga tcaagcagtt gttaattgat ggggtaaatc cagatacgtt acctgtgtac
     1621 tttaaccgga tgatggatgt tagtcaggat attttgatca ttcatagtga tggcatcaat
     1681 aaaattgtta accggacaaa tgtcagtgat gacatgttaa ataacatacc tgctagtgag
     1741 acaatcagcg cagcaggcat ttacagaagc attattaatg atacagagat agatgcttta
     1801 cgaattaaca ttgatgaagt ttcgccatca ttaacggtta ctgtggctaa attggcttca
     1861 gccagacata atatgcttga acagtataaa attaatagca ttataatttg cattgtcgcc
     1921 attatactgt gctcagtatt aagtccgttg ttaatcagga cggggttgcg agagatcaaa
     1981 aagttgagtg gcgtaacgga agcgctgaat tataacgata gccgagtgcc ggttgaggtt
     2041 aacgcattac cgagagaact aaaacctctt gggcaggcgt tgaataaaat gcatcatgct
     2101 ttagtcaaag attttgagcg ccttagtcaa tttgctgacg atctcgctca tgaacttaga
     2161 acgccaatta atgcattact gggtcagaat caggttacgc tcagtcaaat cagaagtatc
     2221 gctgaatatc aaaaaacaat tgccggaaac attgaagagc tggaaaatat ttcgcggtta
     2281 acagagaata tactgtttct tgccagggca gacaaaaaca atgttttggt gaaactggat
     2341 tcgctttctc tcaataagga agttgaaaat cttttggact atcttgaata tctttcagac
     2401 gagaaagaga tttgctttaa ggtcaagtgc aatcaacaaa tctttgcgga taaaatttta
     2461 ctgcaacgaa tgttatcgaa tcttattgtt aacgccattc gatattcacc agaaaa
//
LOCUS       DAJTSI010000284         2439 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000284 DAJTSI010000000
VERSION     DAJTSI010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.290"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..565
                     /locus_tag="OO327_004120"
     CDS             <1..565
                     /locus_tag="OO327_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR6020442.1"
                     /translation="VIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAV
                     ELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTL
                     SERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGA
                     RELNLPSVYGDAAEWNTAELRREMSHS"
     gene            523..1200
                     /gene="bglJ"
                     /locus_tag="OO327_004121"
     CDS             523..1200
                     /gene="bglJ"
                     /locus_tag="OO327_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCR6020443.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAEDILLCMRHCEA
                     SNVLHPY"
     gene            complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="OO327_004122"
     CDS             complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="OO327_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR6020444.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2167..2403
                     /locus_tag="OO327_004123"
     CDS             2167..2403
                     /locus_tag="OO327_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR6020445.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          628 a          534 c          627 g          650 t
ORIGIN      
        1 agtcattgct gatttagccg gtgaaagtga agatccacgt tctgtttgtg aacattatta
       61 ttctttaatc tcacaatatc gggaaattca ctgggttttc atggtgtcac gctcctggta
      121 ttcccaggct gtagaactgc tcatgtgccc tacggcgacg ttattgtctg atgttgaacc
      181 cattgagaat ctggtcaaga ccgtacgttc cggcaatacg cacgcagagc gtatcagcgc
      241 catgctgacc tctccggcaa tgactgaaac tcatgatttt agctatcgct ccgtcattct
      301 cactctttca gagcgcaagg tactgcggct attaggtaaa ggatggggca tcaaccagat
      361 agcttcattg cttaagaaaa gtaataaaac tatcagcgcc caaaaaaaca gtgcgatgcg
      421 tcgactggca attcacagca acgctgaaat gtatgcatgg ataaatagcg cgcagggtgc
      481 aagagaactt aacttgcctt ctgtttatgg agatgccgca gaatggaaca cagccgaatt
      541 aagaagagaa atgtcgcact catagaaaaa tgcgtcatga gtagtatcgg tattgagagt
      601 ttattcagaa agtttgcggg caacccttat aagctccata cctataccag tcaggagtca
      661 tttcaggatg ccatgtcgcg gatctcgttt gcggcggtca ttttttcttt ttctgccatg
      721 agaagtgagc gcagagaggg attatcttgc ctgactgagc tggcgattaa gtttccgcgt
      781 acccggcgtt tagttattgt ggatgatgat attgaagctc ggctgattgg ttcattgtcg
      841 ccatcaccgc tggacggtgt attaagtaaa gcgtcaacgc tggagatttt tcatcaggaa
      901 ctctttttgt cattaaatgg tgtacgtcag gcgaccgacc gactgaacaa tcagtggtac
      961 attaaccaaa gccggacgtt aagcccgacg gagagagaaa tattgcgctt tatgtcgcgt
     1021 ggctactcaa tgacacaaat tgccgagcag cttaaacgta atatcaaaac gatccgagcg
     1081 cataaattta atgtgatgtc gaaactgggc gtcagttctg atgcagggtt gttggaggcc
     1141 gaagatattc tgttatgtat gcggcattgc gaagcaagta atgtgttgca tccctattaa
     1201 tccgcatgat gccgggttta cttccccggc agtgctttca tttcagcgta caatagccac
     1261 attgctgcac atccggtaag cgataacgct ggcagcaagt gcggcgcacc agcaggccgt
     1321 cgcgcagtac cacggtacgc cagagtggat tatcttcacc gttcgtgagc gttttctcaa
     1381 aaaagagggc atggcgcagc gattcaacag tagcctcgcc gagcagttgc ttcatctcag
     1441 tgagatacca gttgatcaaa taaccggtat tactccagat aagtttgccg ttgatctctc
     1501 cggtcgcttc tagtgcttgc acaaccggaa ccagcgcctg gctgattaac gtttccattc
     1561 gctgctgcgg cgaatgtggt gttgcgtttt tatcttcaca cacatcgacc cagaaacagg
     1621 cgacgcgtcc ggtttcgtga aactcagcat ggaaatgttc cggcgacaca tctaatgctt
     1681 tttcctgcgt cagtagcgcc agcattaatg gtggcaccat caggccgata taccattgtg
     1741 cccatagtga gatcagcggt ttgttctcgc ggatcatcgt tggttggttg cgatagatat
     1801 gatcggaata gaccgccagt agagaactta gcgcattcgg tgatgaccat tgcgccagcg
     1861 tcatggcgtt aagcggagcg ggttcatcca ggcggataaa ctccagcaaa tgttcacgat
     1921 gtttggcgat catcgcccgt acggcttgtg caagcccagc atcctgcggc tggagatgcg
     1981 ttcgccagat gacatcttca tagagcggtg cggaacgata ggccataatc gtgatagtaa
     2041 tctaaatgat aatgattgct aatcctagcg ataggtttac tcgatagcaa gggatttctc
     2101 tggcttgcaa atgataaaaa ttatcatatg atattggttt tcattatcaa tgaaagagat
     2161 gaaaccatgt tgcaacgtac gctgggcagt ggctggggag tgttgctgcc gggattgctg
     2221 attgcagggc tgatgtatgc ggatttatcg ccggatcagt ggcggattgt cattctgatg
     2281 ggattagtat tgacgccggt gatgctgtat cacaaacagt tgcggcatta cattttgcta
     2341 ccatcgtgcc tggcgcttat tgctggcatc atgctgatga taatgaattt gaatcaggga
     2401 tgacaaaaca gggaagaaac aagaaaggaa gtaaagata
//
LOCUS       DAJTSI010000285         2433 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000285 DAJTSI010000000
VERSION     DAJTSI010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.291"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..140)
                     /locus_tag="OO327_004124"
     CDS             complement(<1..140)
                     /locus_tag="OO327_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCR6020446.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TK"
     gene            complement(1521..1673)
                     /locus_tag="OO327_004125"
                     /pseudo
     CDS             complement(1521..1673)
                     /locus_tag="OO327_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1729..2422
                     /gene="ospZ"
                     /locus_tag="OO327_004126"
                     /pseudo
     CDS             1729..2422
                     /gene="ospZ"
                     /locus_tag="OO327_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
BASE COUNT          793 a          423 c          437 g          780 t
ORIGIN      
        1 tcttttgttt tgccactcag agatcctaga ctaatatccg tactgatgtt gtccggtatg
       61 aaaagtggat agttagtggt agcatgtgta gtaaagatcg caggtacaca aagtgcaagt
      121 acaaagaatt ttaatttcat cgagaactct acttttttgg ttgaaatgtc catgatcttc
      181 ttactttata agataaacgt caatgccaag cattatttta attaaatatt tgtttatgtt
      241 accaagaaca tatcttttat tataatattt attgaacttt tgtaaaaata aaaaaatacc
      301 tcattgtgcg aataaagtaa cgggggccat agatttctct acctaccttt tggtgttcat
      361 acctgaggct gatagtttca ggattccgcg acaattctac tcaccttctt gttctctcgc
      421 caatatgaaa tcaatcagaa aagcgttcca taagaacaac gataagacaa taaattgtca
      481 tcacggctgc aatagccaac gtgcctttca gtaccaacat gacaacctcc tgtatttgac
      541 gtaacgtgtg ctgataaata tgtatttgta tctcaactga ctcaatacaa cacttaagta
      601 tagtttttca gattttgttt ttcccgtgaa atattatgga aatggcagaa gcaatacatt
      661 gaattccgat tcgagtgaat acggctagcg ggcacctcac tttagcactg aagccatcaa
      721 cttttatttg acttatttca atggaaagct ttctcactat cagtacaggt aattcccatc
      781 cttttaaagt cgagagtcag actgcgacta atacagttaa agtgaccaaa ctcagttatc
      841 ctctacacat tttatctcat gtttacttgg acaattcatt ggtgctttta taaaagaata
      901 aatattctac taatttaatg catcacctaa aatagtattt tttataatag aactgtatct
      961 atagtatatt attaaagctt aattatttat tatgctttaa tgtggttgtc cgattaagga
     1021 ctaatccttg aaaacgccgt atagggcgag agttcactct attgcgataa cgtaaccgta
     1081 caatattgtg cgtttcccaa attgaccact actgaactat tctgggattt aaatgcaata
     1141 aaatggttgg ttgaaggtcg tggtgatgat gttagtcctt ttgagaatat tccagtgcca
     1201 gatcttactt ataataaaga ggcttggcag tttggataag ctattgtcct tattccacta
     1261 atacaaactt tttgtaaagt atattaaata tacttcattt catactgaaa tccccggaag
     1321 tattcaatca aacaattaca ccataaaagg aagagggagc cccaattaag ttatcaagca
     1381 tgtttatgat acctgcaggg tataaatttt ctggctgcaa gcagacacat ataaaacgtc
     1441 tttattgatc ctacccacat aatatggacg cagtcgtaag tgaggctctg tgagcctacc
     1501 cacgtaatat ggacacaggc ctaagcgagg ttctggtttt caaattgttc cggactgagg
     1561 ccgccacacc aacggtgccg tcgccagcga ttgtaattgc actcgatata attaaacact
     1621 gttgcccgca ctatttcccg atagataaag tgttctccgt ggagacattc cacttaggaa
     1681 caattatatt gttcatggta atgtttatat ttgagtatag agattaaaat gattagtccc
     1741 atcaagaata ttaagaacgt atttccaata aatactgcaa atactgaata tatagtgaga
     1801 aatatttatc ctagagttga acatggttac tttaatgaat caccaaacat atatgacaag
     1861 aagtatatct ctggtataac cagaagtatg gcacaattaa aaatagagga gtttattaat
     1921 gagaaatcca gacgacttaa ttatatgaag acaatgtatt ctccatgtcc ggaagatttt
     1981 cagcctattt ccagagccga agccagtaca ccggaaggaa gctggttgac agttatatct
     2041 ggaaaacgtc ctatggggca attttctgtg gatagtttgt accatcctga cttacatgca
     2101 ttatgtgagc tcccagagat tagttgtaag atcttctcta aagaaaatag tgattttttg
     2161 tatataattg ttgtattcag aaatgattct tcccaaggag aactacgggc aaatcggttt
     2221 atagaactat atgatataaa aagagagatt atgcaagtgt taagagatga gtcgccagag
     2281 attaaagtct attaaatctg agattattat tgctcgtgaa atgggagaac tttttagtta
     2341 tgcatcagag gaaatagata gctacataaa acaaatgaat gacaggcttt ctcaaatcaa
     2401 ggctcgaatg cctgttactt aatgtatatt aat
//
LOCUS       DAJTSI010000286         2421 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000286 DAJTSI010000000
VERSION     DAJTSI010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.292"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..836)
                     /gene="gap"
                     /locus_tag="OO327_004127"
                     /pseudo
     CDS             complement(1..836)
                     /gene="gap"
                     /locus_tag="OO327_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            1025..1555
                     /gene="cybB"
                     /locus_tag="OO327_004128"
     CDS             1025..1555
                     /gene="cybB"
                     /locus_tag="OO327_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR6020447.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            1800..1973
                     /locus_tag="OO327_004129"
     CDS             1800..1973
                     /locus_tag="OO327_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020448.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(2045..2194)
                     /gene="hokB"
                     /locus_tag="OO327_004130"
     CDS             complement(2045..2194)
                     /gene="hokB"
                     /locus_tag="OO327_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCR6020449.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(2295..>2421)
                     /locus_tag="OO327_004131"
     CDS             complement(2295..>2421)
                     /locus_tag="OO327_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6020450.1"
                     /translation="ISELKDAVTEYIEYYNSRRISLKLKGLTPNEYRNQTYMPRV"
BASE COUNT          623 a          551 c          566 g          681 t
ORIGIN      
        1 agagactatt tcttcatcgg tataaccaaa tgactcgtta ttggtggttg cttgtttaag
       61 tgcgttattc acctcttcgg cagtcacttt tttccgagaa tggacaccag ctcagtgacc
      121 gaacctgttt tcaccggcac gcgttgcgca tgacctttca gtttgccgct cagttccggg
      181 atcaccagac caatggcttt tgccgccccc gtagtgtggg gaatgatatt ttctgccgct
      241 gcgcgtgaag cacgtaaatc tttaccacgc gggccatcca ccagtgactg ggtgccagta
      301 taggcatgaa tggtcgtcat cgtgccgact tctatcccga aactgtcatg caaggctttg
      361 gccatcggcg caaggcagtt agtggtgcat gacgccacgg aaacaatggt gtcgttgcca
      421 tccagagtgt cgtcattaac gttataaacg atggttttca tttcaccggc aggagcggaa
      481 atcaacacct tcttcgcacc agcatcaaga tgcgcctgcg atttatcggc ggaggtataa
      541 aagccagtac attcgacaat gatttctgcc cctttcgctt tccacggaat atttttagcc
      601 tctttttcgg cgtaaaccgc gatacttttc ccatcaacga taagtgaatc ttccgtaaaa
      661 tcaacgctcc aggggaacgg accatagttt gaatcatgtt tcagcagata ggcgagaatt
      721 tttggggaag taagatcatt aatagcgaca acgtctatgt tgcttttgac ttcaagtaaa
      781 cgacgcaaca ccagtcgacc gatacgacca aaaccgttaa taccaacttt actcatggtt
      841 ttctcctgtc aggaacgttc ggatgaaaat tgatcctttc caggcttaga ccaggattgt
      901 gggatgggca atctccattc ttacagtgaa acgtaacgta ctgaaaacgg gtgaacaata
      961 tttaatgaaa ttttgagaaa agcccgttat gttaacggaa aattatgtta aagcaggaaa
     1021 tgttatggaa aataaatact caaggttaca aatcagcatt cactggctgg tctttttgct
     1081 ggttatcgca gcgtattgcg caatggagtt tcgtggtttc ttcccacgta gcgatcggcc
     1141 gctcatcaac atgattcatg tttcctgtgg catctcaatt ctcgtgctga tggtggttcg
     1201 tcttttgtta agactgaaat acccaacccc gccgattata cctaagccga aaccgatgat
     1261 gacgggactg gcgcatttgg gacatttggt gatttatctc ctgtttattg cgctgccagt
     1321 gattggtttg gtgatgatgt ataaccgggg caacccgtgg tttgcgtttg gtttgacgat
     1381 gccttacgct tcagaggcca atttcgaacg ggttgatagc ttaaagtcgt ggcatgagac
     1441 gctggcgaat ctgggatatt ttgtcatcgg gttgcacgct gcggctgcac tggcacacca
     1501 ctatttctgg aaggacaaca cacttctacg catgatgccg cgtaaacgtt cctgaaggat
     1561 atttaaagaa aacgcctgta ctaaaaccga cccgtggtac aggcgaagaa tacgggtcta
     1621 catcggaagc gcctattata tttatttgta tgacaaataa aaccataatc cttgcccata
     1681 cgtccatctg gcttattttt aatcaattca cccgatcttt gatctcatca acggtatcaa
     1741 aataaaccag cgtataacgt tcaaatcgca cataagattc gataaaccat ggaggttata
     1801 tgaaaaaact ggcacttatt ttgtttatgg gaacgcttgt ttccttttat gccgatgccg
     1861 ggcgcaaacc ctgttctggt tcgaaagggg ggatctcaca ctgtacggca ggcggcaaat
     1921 ttgtctgtaa tgatggttct attagtgcat cgaaaaaaac atgcactaac tgaagtgtaa
     1981 aaggggtgcc atgagaaaat gaatctgcta gtcagatgcg cggggaaaat ccccgcgctt
     2041 gcccttacct ggacgtgcag gccatgagcg cagcaacctc cttatcaccg tcccggaacc
     2101 gcagttcgta gagcgtttgt cgggtcagga gtgtgaatgc cagaatcgta atgcagataa
     2161 tgagcagaca caccaccaga gggttgcgct tcatagcctt tctccttgcc ggatggcggg
     2221 taagaggcta acaatctgaa ttgcttggtt cattcgttgg cctcggtttg tactgacccc
     2281 aaaaagttgg acagttaaac acgaggcata taggtctgat tccgatattc atttggagtc
     2341 agacctttta atttcaggct aattcttctg ctgttgtagt attcaatata ttccgtaaca
     2401 gcatccttca gttcgcttat a
//
LOCUS       DAJTSI010000287         2397 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000287 DAJTSI010000000
VERSION     DAJTSI010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.293"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..178
                     /locus_tag="OO327_004132"
     CDS             <1..178
                     /locus_tag="OO327_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020451.1"
                     /translation="ASLIMVVGCSSKENALCHPQPKPPAPPAWAMMPPSNSLQLLDET
                     FSVSGTESSATRQH"
     gene            complement(311..823)
                     /locus_tag="OO327_004133"
     CDS             complement(311..823)
                     /locus_tag="OO327_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCR6020452.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1018..1782
                     /locus_tag="OO327_004134"
     CDS             1018..1782
                     /locus_tag="OO327_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020453.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1733..>2397
                     /locus_tag="OO327_004135"
     CDS             1733..>2397
                     /locus_tag="OO327_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCR6020454.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          676 a          535 c          608 g          578 t
ORIGIN      
        1 cgcaagcttg ataatggtgg tcgggtgctc gtcaaaggaa aatgccctgt gccatcctca
       61 gccgaaacct ccagcgcctc cggcatgggc aatgatgcca ccgtcgaact ctctccagtt
      121 gctggacgaa acgttctcgg tatccgggac ggaatcatca gcgaccagac agcattgaga
      181 atgcttcagg agtacatcag gatccaatgc cttggggggt agcggtaatt ttactcatta
      241 tccttcaaat cagattctgt tgtcagagga atgggggaag ctggattcgc aagagctttt
      301 tatgggcagt ttagctaaaa aaaggcaaca cgaagctaaa actaccaaca taaagtgctg
      361 accagctagc agctgaaatc agactcactg tatacacttt cactggatga agttgcaaca
      421 ttcctgccac caaaggagca atgtaacgca gcacggcgat aaagcgcgaa gtgaaaagta
      481 ttgacacaga gttattttgc aactgaaggc gaacccgttc tattgtattg gaacggctcg
      541 atattataca tgcaagttga gggatatgcc caattattat accaagatgg taattaacga
      601 tagtaccgca ccatgcgcca ctcatgaccg tgattccagc ttcccatggt gaaagagtcg
      661 tcttgctgac tgtaattaca gtggccatca taattgatgc tggtggcaat atcgccgaaa
      721 tgagtaaagt ggattttgtg aacgctatta taaaaagaag cccccatagg cgatatggat
      781 gaagtacaaa atagttcatc aaagcattta tccactccat caatgtcttt ccttctgtga
      841 atttttcttt gctatttaac cgtgatacga cttaatttag gcttaaacac cttacattta
      901 caaggtactc ctgtgggggg ccctgccacg gagcgtcgca ggagtgagtg atgaggaaga
      961 attaaagcaa cctattgtga caggcagcga taacagtaaa tcacaggaag cataatcatg
     1021 gcaaaaccgg actgggaggc catcgaatcg gcataccggg ccggagtcct tagcctccgt
     1081 gatataggcg agaaatacgg cgttacagaa ggggctatca ggaagagggc caaaaagctt
     1141 gattgggcac gcagtggcgg tacgcaggtt tgcaaaaatg gtacgcaaaa aaggaaagtg
     1201 cgtaccagca gaaagcctgc cattactggc cttacacaaa aaagtacgca actaaaaaca
     1261 gaatctacac cggatacgaa accgatacgc ggaatgcgta ccgatccccc gactaaccca
     1321 ttccaacccg gtaaccagca ggcattaaaa cacggtggtt atgcccgtcg ccttctgctc
     1381 aaagatgagg tgatagagga cgctaaagcg ttgacgctcg aggacgaatt atttcgcctt
     1441 cgtgctaaca accttgtcgc tgcagagaat attggtcggt ggttggtgtc gctggaagat
     1501 gctaatgggg accaggaaag gaagatgctg attgaaaata tcagcgccgc cgagaaagca
     1561 atgatgcgca atacagttcg tattgagtcc atcgttggca cgcttgcgac ggtaggaaaa
     1621 atatttgctg atacagccta ccgcaaggcc gccactgata aggtgtctct ggaggctgat
     1681 cgtcttcgcc gtgatgcagg tattgatgat ggcaatggag agcgtgacct caatgacttc
     1741 tactctgaca tccaaaccga cgctgaatcc ggctttacgt agtttctgga ctatgcgggc
     1801 acgtaacaaa gtgctttatg gtggtcggtc atcgtcaaaa tcatgggatg ccgctggcat
     1861 tgccatattt ctgtcgaata aatacaccct gcgtttttgt tgtgcccgtc agatccagaa
     1921 taaaatcgaa gagtcggtgt ataccctgct caaaattcag atagacaggt ttggtctgcg
     1981 gcaccgtttc cgtattctga acaacaaaat cattaaccgg gttactggct cggaatttgt
     2041 tttttatgga ttatggcgca acatcgaaga aattaagtca ctggagggga tcgatatgtt
     2101 gtggttggaa gaagcccacg cactgacgga ataccagtgg aaaattctgg agccaacgat
     2161 ccgtaaagag ggttcggaat gctggttcat attcaacccc ggacttgtta ctgatttcgt
     2221 ctggcgcaac ttcgttgttg atccgcccga aggcactctc atccgcaaaa ttaactatga
     2281 cgaaaatccg tttctgtctg acaccatgct taaggttatc gacgcggcgc gacgccgtga
     2341 tccggatggt tttaaacatg tgtatgaggg cgttccggag tctgatgatg atgcggc
//
LOCUS       DAJTSI010000288         2363 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000288 DAJTSI010000000
VERSION     DAJTSI010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.294"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..813)
                     /locus_tag="OO327_004136"
     CDS             complement(<1..813)
                     /locus_tag="OO327_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6020455.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDY"
     gene            complement(1414..1662)
                     /locus_tag="OO327_004137"
     CDS             complement(1414..1662)
                     /locus_tag="OO327_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR6020456.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2017..>2363)
                     /locus_tag="OO327_004138"
     CDS             complement(2017..>2363)
                     /locus_tag="OO327_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6020457.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          611 a          434 c          570 g          748 t
ORIGIN      
        1 gtagtccggc gaagatgtta atctttgcag ggattgcaga gcatgggatg acaatgggtt
       61 atcactaata tctattgaac attcattcct cagattaaga atgctttccg ggatatgact
      121 tatctgattt atgtcaaaat aaccgttcct taaattttcc ggcaggtatg gaatatcggt
      181 taacatattg tcgattgccc atatggagtg aatgttgtga ggtagagggg ggagttcact
      241 aagaagattc cctattgcgc taatctcttg taaagaaaat ggtaaaggtg gtaaaactgc
      301 cagcccatta catgatacat ctaatgtttc cagtagttct ggtaaaacag gaagagaaca
      361 taattgatta tttgagacat gaagctcctt caaagatatg gggagtgtgg gtagtgtgat
      421 tagttgattg tgggacgcat tcaataattt aagtccttga ggcaaagcag gcagttcaat
      481 aagtctgtta tagctgacat taagttgtgt aagggacgca ggcaatgggg agattaagct
      541 taaattattt ttacttatat taattgattt aattcccggg gggatttcag gtaatgttgt
      601 caggcctaat tcagacaagt ccaggttcgt ctcttggttt tgtagacatg atactagtcg
      661 ctgaaaagcg atgtctcgtt gttcttcttg tatgcggtta tttttccatt cagtccaatg
      721 ggttagataa ttttcatatg cgcggctagt gtcgattgaa taagtggaga acgaatttga
      781 aattaatctg tgattgttat ttgtcgggag cataaatatc agggtgtatt ctttatgttg
      841 agaggtgttg gattcttttt tatctgcttt gcaatgttat tggttccctt cagcagtaga
      901 caggaacttc tggaggaggc ggatatatag tttgtgtaag catattaact ccatatgtta
      961 tatattgaag aacttctgct ttacactatc ctaaactgga tcggtttaat gtaaaaaaac
     1021 gatacttttt gggggggggg gcaatttttc cttaaggtat gaaaaaagga gctgaagcta
     1081 tataatagct attaatgcca ctggtatcag tgaatcatgt atataaaaaa ctgtgttaag
     1141 gaggctgtcc attagcggtt ttttattctt ctggaaccgc ggcatctttg cgtatctttt
     1201 tgtcttctca ccgtctggtc ggtgttttgc tgagactgct aatttcctgt ctttgctagt
     1261 gctgtactgg tactgcccaa tcatgattgg cgggggcggg cggagttgag accgcagaca
     1321 cgtcgtatgc aagaacgtgc tgcggctggc tggtgaactt ccgatagtgc gagtattgaa
     1381 tgatttccag ccgttactga ttttacgtgc taattagtga acaaaccact cgtcagcaga
     1441 ctcccaggta tctttcagag tttcctgaac aaaagtttta gctgaatctt tatcggcggt
     1501 gcgcgtaaca gaaaggccat cgttgctggt ggcttttacg atcacctcta catcgtcata
     1561 acgtttactg atgcgtcggg ttaattcttc ctttaacgca tccacagcac cggttggcat
     1621 tttagtcatt ttctctttgg ctatgcagat ttcaaaacgc ataaaaatcc ctctgcactg
     1681 tgtttttata cagtattatt tttaactgta tggataaaca gtgtcaagag tgagggaaag
     1741 tgattttttg gggcatgcat ggggcaaaaa tatttgttgt ggggcatttt ggggcatgat
     1801 tgggacactt tgttacatat gaactttgcc gattttcata tgacatggca atccaacgct
     1861 ttgattattc taaagaaaac acatgctctt gggcgttctt tagtgatttt taaaaatccc
     1921 gtatcacgca gttaaagtgg cgggcatact cttcaaggct ggtgatcccc aaccgcaccc
     1981 atttaggatg tgaccattgc ggtagaccga tgtaaatcat aacgcgtgaa ggatctcttc
     2041 cacgctacgc acacgggcga tgcgcgggta gatatgatta atgctgttat tgtgttgctc
     2101 ggcgctggcg gcactacagg cgtcttcggc aatcaccaga ttaaagccaa gttcccaggc
     2161 attgcgggcg gtggattcaa caccgatatt ggtcgagatc ccacataaca ctattgtatc
     2221 gatacctcgg cggcgcagtt gcaactccag atccgtaccg taaaacgcac cccactgacg
     2281 tttgatgatt tcgatatcac tgtcagttgc acctaatgca gctggatgtt gccaccagtt
     2341 ttcgggcaac actttagcgg ggg
//
LOCUS       DAJTSI010000289         2334 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000289 DAJTSI010000000
VERSION     DAJTSI010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.295"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..171
                     /locus_tag="OO327_004139"
     CDS             <1..171
                     /locus_tag="OO327_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6020458.1"
                     /translation="IRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTP
                     KRYRKKLTEFNQ"
     gene            276..1628
                     /gene="celF"
                     /locus_tag="OO327_004140"
                     /gene_synonym="chbF"
     CDS             276..1628
                     /gene="celF"
                     /locus_tag="OO327_004140"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR6020459.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1641..>2334
                     /gene="chbG"
                     /locus_tag="OO327_004141"
     CDS             1641..>2334
                     /gene="chbG"
                     /locus_tag="OO327_004141"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCR6020460.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLT"
BASE COUNT          601 a          474 c          620 g          639 t
ORIGIN      
        1 atccgtatta attttgcaaa aaaacaactg gaaatgacca actattccgt gacggatatt
       61 gcgtttgagg ccggttatag tagcccgagt ttgtttatta aaacgtttaa gaaattaacg
      121 tcctttacgc ctaagagata tcgtaagaaa ttgactgaat ttaatcagta agtcgttata
      181 cctgacaatt cacatatctg tcctgttgct ggattattta tgtccggggg ggcagatatg
      241 ccagatatca gtattctgta ctgaagggag aaattatgag ccagaaatta aaagtcgtca
      301 ctattggtgg cgggagcagc tataccccgg agttactgga aggatttatt aagcgttatc
      361 acgaattgcc ggtcagcgaa ttatggctgg tggatgtcga gggtggcaaa gctaaactgg
      421 atattatttt cgatctctgc caacggatga ttgataacgc tggcgtcccg atgaagcttt
      481 ataaaacgct ggattgccgc gaagcattga aagatgctga tttcgttact acccaactgc
      541 gcgttggcca attaccggcg cgtgaactgg atgaacgtat tccattaagt catggttatc
      601 ttggtcagga aaccaacggc gcgggcggtt tgtttaaagg tctgcgtacc attccggtga
      661 tttttgacat cgtaaaagat gtcgaagaac tttgtccgaa tgcatgggtg attaacttca
      721 ctaacccggc gggaatggtt actgaagccg tttatcgtca taccggattt aaacgcttta
      781 tcggcgtgtg taatattccg atcggcatga agatgtttat tcacgatgtt ctgatgctga
      841 aagacagcga tgatttatct atcgatttgt ttggcctcaa ccatatggtg ttcattaagg
      901 atgtgctggt aaatggcaag tcgcgctttg ccgaattgct tgatggtgtg gcatccgggc
      961 agttaaaagt atctggcgtt aaaaatattt tcgatctgcc atttagcgaa ggcttaattc
     1021 gttctctgaa tctgttgccg tgttcttatt tgctttatta cttcaagcaa aaagagatgc
     1081 tggctattga aatgggcgaa tactacaaag gcggcgcacg agcgcaggta gtacagaaag
     1141 tcgagaaaca actttttgag ctgtataaaa acccggagtt gaaagttaag ccgaaagaac
     1201 tggaacagcg cggtggggct tattactctg atgcagcgtg cgaagtgatc aacgctatct
     1261 acaacgacaa gcaagctgaa cattacgtta atatcccgca tcatgggcat attgataata
     1321 ttccggcaga ctgggcggta gaaatgacct gtaagctggg gcgcgatggc gcgacgccac
     1381 atccgcgcat tacgcatttc gatgataaag tgatggggct gattcacacc attaaaggct
     1441 tcgagattgc tgccagcaac gccgcactta gcggagaatt taacgatgtg ttactggcgc
     1501 taaaccttag tccgttggtg cattccgatc gcgatgctga gctgctggca cgcgagatga
     1561 ttctggcgca cgagaaatgg ctgccaaatt ttgccgactg catcgcagag cttaaaaaag
     1621 cacattaacc gaggctgatt atggaacgct tactgattgt taatgccgat gattttggct
     1681 taagcaaagg tcagaactac ggcattatcg aggcctgtcg caatgggatt gtcacatcga
     1741 cgacggcgct ggtgaatggg caggctattg accatgcggt gcagttgagt cgtgatgaac
     1801 cgagtctggc catagggatg cactttgtcc ttactatggg caagccactg acagctatgc
     1861 cggggttaac ccgcgatggt gtgctgggaa aatggatctg gcagttggca gaagaagatg
     1921 ctttaccgct ggaagaaatt actcaggagc ttgccagtca gtatttgcgt ttcattgagc
     1981 tatttggacg caaacctacg catcttgata gccatcatca tgtgcatatg ttcccgcaga
     2041 ttttcccgat tgttgccagg tttgcggctg aagaggggat tgcgttgcgc atcgaccgtc
     2101 agccgctatc taacgctggt gatttaccgg ctaatctgcg cagttcgcag ggattcagta
     2161 gcgcgtttta tggtgaagag attagcgagg cactgttcct gcaagtgctg gatgacgcaa
     2221 gccatcgggg tgatccttcg ctggaggtga tgtgtcatcc ggcgtttatc gataatacta
     2281 tccgtcagag tgcttactgt ttcccacggt taacggagct ggaagtgctg acat
//
LOCUS       DAJTSI010000290         2327 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000290 DAJTSI010000000
VERSION     DAJTSI010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.296"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            63..284
                     /gene="kil"
                     /locus_tag="OO327_004142"
     CDS             63..284
                     /gene="kil"
                     /locus_tag="OO327_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR6020461.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYILHEGRTYIASANNIKKRKLYIR
                     SLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            278..454
                     /locus_tag="OO327_004143"
     CDS             278..454
                     /locus_tag="OO327_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020462.1"
                     /translation="MVMTKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYC
                     SEICAEKDQMAHEL"
     gene            529..804
                     /locus_tag="OO327_004144"
     CDS             529..804
                     /locus_tag="OO327_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020463.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            904..>2327
                     /locus_tag="OO327_004145"
     CDS             904..>2327
                     /locus_tag="OO327_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCR6020464.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYMDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDKFITMPGGMDYSRAI
                     VVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEE
                     GKQDDEEKTQPSGAMADEQATAETMEPDATEHHQNTQPLDAQSQVNSVDEKYQELRAE
                     LHEARKNIPPKNPVDADKLLAALR"
BASE COUNT          720 a          532 c          529 g          546 t
ORIGIN      
        1 gctaagtatt aaataacttt cctgagattt cctttgtaag actgtttatt aaggaggctt
       61 ccatgattgc tcatcacttc ggaactgatg aaataccacg tcagtgtgtg actcctggcg
      121 attatattct tcatgaaggt cggacatata tcgcctcggc aaacaatatt aaaaagcgaa
      181 aactttatat tcgtagcctg actacaaaaa catgcatttc tgactgcatg attaaagtct
      241 tcctcggtcg tgatggttta cctgtaaagg cggagtcatg gtaatgacta agaaaataaa
      301 atgtgcttat cacctttgca ataaagaaat tgaagaaagc aaaatcatta caagaccact
      361 tcatttcatg cgtggagtta taccaacgac ggaaatgaaa aaatattgta gtgaaatctg
      421 tgccgaaaaa gaccagatgg cacacgaact ttaattaact gactatccga aactgaattt
      481 atgccagcaa tggcagggat tcgctcaacc ttaattaagg agaaaaacat gattaccagt
      541 tatgaagcca ctgttgtaac tactgatgac attgttcacg aggttaatct ggaaggaaag
      601 cgtattggct acgtgattaa gacagaaaat aaagaaaccc cattcactgt ggttgatatc
      661 gacggtccat caggcaacgt taaaacactt aacgatggtg taaaaaaaat gtgtctggtg
      721 cacataggaa agaatctgcc cgcagaaaaa aaagccgaat ttctggcaac tctgattgca
      781 atgaaattaa aaggtgaaat ctgaaaaaaa gaaagcctgc acactgtgca ggcctgaatg
      841 aagaacctgg gacatttatt catcactcgc agtaatttta atctgagttg aggttaaaaa
      901 acaatgaaca ccgataaaca agtttaccca ctgtattacg aagcaaaaaa tgacaaagta
      961 agaaaacgtc tcggtattaa aggcggtttt tactgggctg aagcgaaaaa attatccatt
     1021 gccatctccc gtggtgctgt tgcgattgac gatgcaggct acgatgaaga tgactttaaa
     1081 aaacctgttc gcgtcaattt gcccgttgtt gatgacctcc cgccagaagg cgtatttgat
     1141 acggaattct gcaaccgtta cgaaaaaggc ggggaagatg gcatcacaat ggtatttatc
     1201 gcatcttctc cctctgttca ggacaaaccg gccagcactg acaataccaa cgtcaacggc
     1261 gaagacatga cagaaattga ggagaacatg ctcctgccag tttccggtca ggaactgccc
     1321 attcgctggc ttgcgcaaca cggcagcgaa aaaccagtaa cgcacgtttc acgcgacgaa
     1381 ctccaggcat tacacattgc tcgggatgaa gaactaccag ctgttactgc cctggctgtt
     1441 tcccacaaaa ccagcctgct cgatccgctg gaaattcgcg atctccacaa actggtgcgt
     1501 gatactgaca gagttttccc taatccaggc aattcaagcc tggggctgat gactgctttt
     1561 ttcgaagcat acatggacgc agactacacc gatcgcggtc tgctgacaaa agagtggatg
     1621 aaaggaaatc gtgtttcacg catcacgcgc acggcttccg gcgctaatgc tggcggcggg
     1681 aacctcaccg atcgcggcga aggtttcgtt cacgatctga cgtcactggc gcgcgacgta
     1741 gccactggcg tactggctcg ttcaatggac gtggacattt ataaccttca tccggcacac
     1801 gctaaacgtg tcgaggaaat tatcgctgaa aataaaccgc ccttttctgt tttccgcgac
     1861 aaattcatca ccatgcctgg cgggatggat tattcccgcg ccatcgtggt tgcgtcagtg
     1921 aaagaagcac cgattggtat cgaggtcatc cccgctcacg tcactgaata tctgaacaag
     1981 gtactgactg aaaccgatca tgccaaccct gatccggaaa tcgtggatat tgcctgcggt
     2041 cgctcctctg ccccgatgcc gcaacgtgta acagaagaag gaaaacagga tgatgaagaa
     2101 aaaacgcagc catctggcgc aatggcagat gaacaggcaa cggctgaaac aatggaaccg
     2161 gatgcaactg aacatcatca gaacacgcag ccgctggatg ctcagtcaca ggtaaattct
     2221 gttgatgaga aatatcagga actacgggca gaactccatg aagcccggaa aaacattccg
     2281 cccaaaaatc ctgtcgatgc agacaaatta ctggctgcct tgaggta
//
LOCUS       DAJTSI010000291         2326 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000291 DAJTSI010000000
VERSION     DAJTSI010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.297"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..122
                     /locus_tag="OO327_004146"
     CDS             <1..122
                     /locus_tag="OO327_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCR6020465.1"
                     /translation="QTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(164..1603)
                     /gene="aldA"
                     /locus_tag="OO327_004147"
     CDS             complement(164..1603)
                     /gene="aldA"
                     /locus_tag="OO327_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCR6020466.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(1800..>2326)
                     /locus_tag="OO327_004148"
     CDS             complement(1800..>2326)
                     /locus_tag="OO327_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR6020467.1"
                     /translation="TTGRAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITL
                     LNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVI
                     FINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKH
                     DAVLIEAMESRSLR"
BASE COUNT          580 a          621 c          540 g          585 t
ORIGIN      
        1 cgcaaacgga aattaccgcc gtgggcgatt tacaactggt gaaaacggtc gcctggtacg
       61 ataacgaata tggcttcgtc acgcagctta ttcgcaccct cgaaaaattc gctaaactct
      121 gacgcgcaca ggcggaggaa aaaacctccg cctctttcac tcattaagac tgtaaataaa
      181 ccacctgggt ctgcagatat tcatgcaggc catgtttacc atctgcgccg ccaataccgg
      241 atttacgcca tccggcgtgg aaaccttgca tagcttcgaa gttttcacgg ttgatgtaag
      301 tttcaccaaa cttcagccct ttaatggctt tcatcgcgac gttcagattt tgggtataga
      361 ttgatgaggt caggccgtaa tcactgtcat tagccattga gatagcatct tccagcgtgt
      421 caaatgcgac aactggcagc accgggccaa aggtttcctc atgcataatc gacatttcct
      481 ggcgaacatc cagcagcaat gtcggcggat aataatatcc tttcccctct accgctttgc
      541 cacccaacgc cactctcgcc ccttcttcta ctgcgcgcgc cactttttgc tcaacccttt
      601 ccagcgccgc ggcgttaatc aacggcccca tcgcaatgtc gttgcgttca gcggggttac
      661 caaattgaac cgcctgcaac gcttcaccca gcagattgac gaactgatca taaatgcctt
      721 tctgtacata aacacgttct gcacagttac acacttgccc actattaatg acgcgtgaat
      781 caacgatggc tttgactgcc agttcaagat cggcatcgtc cattacgata gccggtgctt
      841 taccacccaa ttccagacac actttggtga tgtttttcgc cgcagtcgcc atgatcttct
      901 cacctgcaga gacgctgcct gtcatactga ccattgcgac ctttgggtta cccgccagtt
      961 cttgcccaac ggtttcacca cgccccagta caaggttaaa cacgccgcgc ggaaggccta
     1021 tttcatcgac gattttggcg aatgcaatcg cattgtttgg cgtaaattca ctgggtttaa
     1081 tgacgatggt attaccggtc aaaagagcgg gagccatttt gcgggcaatg aggaagaacg
     1141 gaaagttcca cggcagaatg ccggtagtca caccaagcgc acgtttaaac agaagaatat
     1201 tttctcccgg acgatcgctt tgaataatct cgccctcgta acgccgtgcc cactccgcca
     1261 tgtaatcgat atagtcggca gtaaaagcca cttcgacttc agctagctgc tggatcttgc
     1321 ccccttcttc aacaatcagc gcactgattt cactggcgcg ttcgcggatc ccggcggaga
     1381 ttttgcacaa ccaactggcg cgttcaatag caggcaacgc ttcccattct ggttgtgcac
     1441 gttctgctgc atcgattgcc ttacgggcat cctcggcctg accatcgggt atgcgggaaa
     1501 tgacagcctc tgtagcaggg tttaccacat caatccatgc gtctccacgc caggtaacaa
     1561 actgtccatc gatatacata ggatgttgaa cgggtactga catgggcgac tcttgtgatt
     1621 tatatgtttg ttttcggtga atatattgtt aattaacatc aatcatcaga gagacggcat
     1681 cgggaaagga caagtctgtg gagggcttca taaaaacaag tgtaaaagga ataaaaggca
     1741 gaaataaaag tcaggagtca tgcccgtttg caaacaagtg gttcacaaac gggcaatttt
     1801 taacgtaacg agcgactttc catcgcctcg atgagcaccg catcatgttt cagcgtttgc
     1861 catgcatgga tgacttctgc cggaaaatca acgtgaacga taaaatctcg cccgcgggga
     1921 ccgtagccat cgctatcatc gtgtaaacgc ggcagctcgc cagtgagtag tgagagataa
     1981 cgctcaaccg gccataatcc ttgtaactga ttaataaaga ttacactgtc tgcgttattt
     2041 cctaactgag gaacgaatcc gggataactt aaccagcgtg gtgcatcggg attgtccccg
     2101 gtcatacggc ggaacgttgc catcgtgcgc cgctgcatgg tggggtcctg tacaacgata
     2161 gccgtatgaa ctcgttctac ggcctgattc aatagcgtga tgctaaagcg tgcgttttca
     2221 ccgcagtttg ttgactggtc ttcaatccag attttttcat gcggaatatg ccagaactga
     2281 tgagcgatat ccaccaggat ggtcgcttct gctctgccag tggtgc
//
LOCUS       DAJTSI010000292         2317 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000292 DAJTSI010000000
VERSION     DAJTSI010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.298"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..256
                     /gene="mliC"
                     /locus_tag="OO327_004149"
     CDS             <1..256
                     /gene="mliC"
                     /locus_tag="OO327_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCR6020468.1"
                     /translation="ERMQTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQLLHLKQGLS
                     ASGARYSDGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
     gene            315..971
                     /gene="pdxH"
                     /locus_tag="OO327_004150"
     CDS             315..971
                     /gene="pdxH"
                     /locus_tag="OO327_004150"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR6020469.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1100..>2317
                     /gene="tyrS"
                     /locus_tag="OO327_004151"
     CDS             1100..>2317
                     /gene="tyrS"
                     /locus_tag="OO327_004151"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR6020470.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFG"
BASE COUNT          585 a          566 c          614 g          552 t
ORIGIN      
        1 tgagcgaatg caaaccgata cgctggaata ccagtgtgat gaaaaaccgt tgacggtcaa
       61 actgaataat ccgcgccagg aagtcagttt tgtttacgat aatcaactac tgcatctcaa
      121 acagggcctt tcagcctctg gcgcgcgtta ctccgacggg atctatgttt tctggtcgaa
      181 aggtgaggaa gcgactgtct ataaacgcga ccgcatcgtc ttgaataact gtcagttaca
      241 aaatccacag cgttgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat
      301 ccgtaaagaa aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg
      361 aatacaccaa aggcgggtta cgccgccgcg atcttcccgc cgatccatta accctttttg
      421 aacgttggct ctctcaggct tgtgaagcca aactggcgga ccctaccgcg atggtggtcg
      481 ctaccgtgga tgaacatggt cagccttatc agcgcatcgt tttactcaaa cattacgacg
      541 aaaaaggcat ggtgttttac accaacctcg gtagccgtaa agcacatcaa attgaaaata
      601 atccgcgcgt tagcctgctg ttcccgtggc atacccttga acgccaggtg atggtgatcg
      661 gtaaagcaga acgactttcg actctcgaag tgatgaaata ttttcatagc cgcccgcgtg
      721 atagccagat tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc
      781 ttgaaagtaa attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccga
      841 gcttttgggg cggttttcgc gtcagccttg aacagattga gttctggcag ggcggtgagc
      901 atcgcctgca tgaccgcttt ttgtaccagc gtgaaaatga tgcgtggaag attgatcgtc
      961 ttgcaccctg aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac
     1021 tttattctat gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt
     1081 tatatacatg gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg
     1141 gctggtagcc caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat
     1201 cgcgctctat tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc
     1261 attgttatgc ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg
     1321 cgcgacgggt ctgattggcg acccgagctt caaagctgcc gagcgtaagc tgaacaccga
     1381 agaaactgtt caggagtggg tggacaaaat ccgtaagcag gttgccccgt tcctcgattt
     1441 cgactgtgga gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa
     1501 tgtgctgacc ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa
     1561 agaagcggtt aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc
     1621 ctacaacctg ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg atgtggtgct
     1681 gcaaattggt ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg
     1741 tctgcatcag aatcaggtgt ttggcctgac cgttccgctg atcaccaaag cagacggcac
     1801 caaatttggt aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta
     1861 caaattctac cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt
     1921 cttcaccttt atgagcattg aagagatcaa cgccctagaa gaagaagata aaaacagcgg
     1981 taaagcaccg cgcgcccagt atgtactggc ggagcaggtg actcgtctgg ttcacggtga
     2041 agaaggttta caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ctttgagtgc
     2101 gctgagtgaa gcggacttcg aacagctggc gcaggacggc gtaccgatgg ttgagatgga
     2161 aaagggcgca gacctgatgc aggcgctggt cgattctgaa ctgcaacctt cccgtggtca
     2221 ggcgcgtaaa actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc
     2281 tgaatacttc tttaaagaag aagatcgtct gtttggt
//
LOCUS       DAJTSI010000293         2308 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000293 DAJTSI010000000
VERSION     DAJTSI010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.299"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..400)
                     /gene="narZ"
                     /locus_tag="OO327_004152"
     CDS             complement(<1..400)
                     /gene="narZ"
                     /locus_tag="OO327_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="with NarYV catalyzes the reduction of nitrate; the
                     beta subunit is an iron sulfur cluster containing electron
                     transfer subunit; one of 3 nitrate reductases in E. coli;
                     expression of nitrate reductase Z is not dependent on
                     nitrate levels; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR6020471.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPV"
     gene            complement(482..1870)
                     /gene="narU"
                     /locus_tag="OO327_004153"
     CDS             complement(482..1870)
                     /gene="narU"
                     /locus_tag="OO327_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCR6020472.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
BASE COUNT          682 a          533 c          562 g          531 t
ORIGIN      
        1 gtaccggatc gctgtgttgt ttgagggctt cgcgccacag ttcaatcagt cgtttacgaa
       61 tgagcgggta tttcaggcgg ttagcgctgt aaagatacca ggagtaactt gcgccacgcg
      121 ggcagccgcg aggttcatga ttgggcaggt cagggcgagt gcgcgggtag tcggtctgtt
      181 ggatttccca ggtcaccaga ccatttttaa cgtagatttt ccagctacag gagcctgtac
      241 agttaacgcc gtgggtggaa cgcacgattt tgtcgaactg ccaacgctgg cgatagctgt
      301 cctcccagtc gcggttgcta tgcatcacct gtccgtgacc atcggcaaag gtttcgccct
      361 tttgtttgaa gtagcgaaag cgatccaaaa gtttactcat gacatgactc ctgctccagg
      421 aatgaattgg gatctgatgc cttgtcggat gcgacatggt tgtacatcag gcatcgatct
      481 cttatttttg gctgaacttc cgccgaccat aaaccagcca ggtcagcagc acacaaacga
      541 tgtagaagat taaaaacact ttcatcgcgc cgaccggaga gccggtcata ttgagcgaca
      601 tgccaaatgc ctgcggaata aaaaagccgc ccactgcgcc aatggctgag ataaagccca
      661 gcgttgccgc cgtttcggtg acggcttctc tctgagcttg ctcatcacta ccgcctttca
      721 tcttcacccg ataaatggtt atctggcgaa agatgacggc gatcatctgg aaagtagaac
      781 cacttcccag acccgcggtc agaaacagcc ccataaatac ggcgtaaaag gcgatgaaat
      841 taccggagcc tgtgcccggt aaggtaagga acagcagggc actgaaaatc gccataaaaa
      901 taaagttgat caacgtcacc cgcacgccgc cgaatttatc ggaaatagca ccaccaaccg
      961 accgcgcgat ggcaccaata aatgggccaa agaacgccag gcgcagaata ttcacatccg
     1021 ggaactgggt ttttgccagc atggcaaaac ccgcagaaaa accgataaac gaaccgaagg
     1081 tggcaaggta aagcaggctc agcagccaga gatgcaggcg ttgtaagaca gggagctggt
     1141 cggcaattga ggcgcgtgaa ctggcgatat cattcatccc tgaccaggcg gcgatcgtgg
     1201 caatcgccag tagcggtacc caaatccatg cggcattcgc cagcgacatc accgaaccgt
     1261 cggcctgcgg tacgccattg acgccgagaa aggcaaatac aggtacaaaa atgaccagcg
     1321 gtgcaaccag ctgcattaca cttacaccta agtttcctaa tccgccatta atcccaagag
     1381 cgctcccttg tttggctttt ggaaagaaga aactgatatt gcccatgctc gaagcaaagt
     1441 ttgcacctgc aaaaccgcat agcaaagcga taacgataaa tatcccaaaa ggagtattcg
     1501 gattttgcac ggcaattccg agccagacgc aaggaataat caggattgca gtactaaaaa
     1561 ccggccatcg gcgtccaccg aatataggca ccataaagga gtagggaaca cgcaataatg
     1621 cgccagaaac ggagggtaat gcggttaata aaaagagttg atcggtagta aaattaaaac
     1681 cgattttatt gagattaacg gtaactgcgc taaatagcat ccagacacag aaggcaagaa
     1741 gtagacaact gactgatatc cagaggtttc ttcgagcaat atgttttcct ttattttccc
     1801 agaaggccgg attttctggt ttccagtcgc gtaaaagata acgactattt ttctcatttt
     1861 gcagtgccat attgttcctc atatgcacac attggtaatg aaaaaagaca aaacaggagg
     1921 taaggcgcaa tagccagtta ttagaattaa ggatgaattg ggtgaagtgc tgattgaaag
     1981 aatagataag aaagcgtaac cgcgggggca gaatggggat taagtagcca gatatgtgtt
     2041 actaaatgta actaacaagc taaccttcaa acgggggtta atctttgaac gtatgtcatg
     2101 tttcaaccct tcagatcgtg aatctaaagg gttacatatt aactatattc tcaacaagac
     2161 cagacactta tagaattagt gatgatttat tcactaaaaa ggccatgaat tggaggaaaa
     2221 cgatctgtcc agaaagtcta tcggttcctc tcttattgcc tgattgtaat aatcgttatt
     2281 atagtaagta ttgtttattt catctaag
//
LOCUS       DAJTSI010000294         2219 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000294 DAJTSI010000000
VERSION     DAJTSI010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.300"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(100..525)
                     /gene="arsC"
                     /locus_tag="OO327_004154"
     CDS             complement(100..525)
                     /gene="arsC"
                     /locus_tag="OO327_004154"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCR6020473.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(538..1827)
                     /gene="arsB"
                     /locus_tag="OO327_004155"
     CDS             complement(538..1827)
                     /gene="arsB"
                     /locus_tag="OO327_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR6020474.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1881..>2219)
                     /gene="arsR"
                     /locus_tag="OO327_004156"
     CDS             complement(1881..>2219)
                     /gene="arsR"
                     /locus_tag="OO327_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR6020475.1"
                     /translation="PIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQPKISR
                     HLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRNLARQN
                     CSGDSKNICS"
BASE COUNT          589 a          611 c          556 g          463 t
ORIGIN      
        1 gaatttgcac ggttttgtag gtcggataag acgcgttagc gtcgcatccg acataaacaa
       61 agcgcacttt accaactatc ggcagtggat atcgccgctt tatttcaggc gcttacccgc
      121 ttcatcaacc actttctcgc catcttcctt ggagaatgcg cctttttgcg catctggcag
      181 aatttccagc accacttctg aagggcggca caggcgagtt cccagcggcg tcaccacaat
      241 cgggcgatta atcagaatcg ggtgctgaag cataaagtcg attaaccgat cgtcagtaaa
      301 tttatcttcc gcaaggccca gctcctcata cggttcgacg tttttacgca gcagcgcgcg
      361 tacggaaatc cccatatcgg caatgagttt gaccagttca tcgcgcgttg gcggagtttc
      421 cagataatgg ataatggtcg gttctgtgcc gctgttgcgg atcatctcca gcgtattacg
      481 cgacgtgccg caggccgggt tgtgataaat ggtaatgttg ctcatatcag tatctcatta
      541 caaagtgaaa gagagacgta gcgccagcgc agccagcgtc acaaacagca caggcagggt
      601 catgataatc cctgtacgga agtaatatcc ccagctgata gtcatattct tctgcgaaag
      661 tacgtgcagc cagagcagcg ttgccaggct accaatcggg gtaattttcg gccccaaatc
      721 gcagccaatc acgttggcat aaaccatcgc ttctttgata acgccagatg ccgtgctgcc
      781 atcaatggac aacgcgccaa ccagtaccgt cggcatattg ttcataatag aagagaggaa
      841 ggcggtgagg aatccggtgc cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt
      901 gagtacgcca gaaagatatt ccgttaatcc ggcattgcgc aggccataaa ccaccagata
      961 catgccgagc gagaagatga caatctgcca gggggcaccg cgcaggactt tacccgtatt
     1021 aatcgcatga ccgcgtttag cgacgacaaa taatatcagt gcgcccacag ctgcaatggc
     1081 gctcaccgga atgccgagcg gttccaggac gaaaaatccc accagcagaa gcagtaaaac
     1141 aacccagcca gttttgaacg tagcaggatc tttgatcgct tctgcgggag atttcagcag
     1201 cgccatatcg tagttctgcg gaatatcttt gcgaaaatag agatgtagca tcaccagcgt
     1261 ggcaacaatc gcggcgatat ccaccggcac catcaccgag gcgtattcgc gaaagccgag
     1321 gccaaagaaa tcagcggaaa cgatattcac caggttggag acaataagcg gcaggctggc
     1381 ggtatcggca atgaatccgg ccgccatcac gaacgccagc gtcgtgcctt tactgaatcc
     1441 taaagccagc agcatggcga tgacaatcgg cgtcaaaata agcgccgcgc catcattggc
     1501 aaacagggcc gcaacggcgg caccgagcag gacaatccag gtaaacagca agcgaccgcg
     1561 tccattgccc cagcgtgaaa cgtgcagcgc tgcccattca aaaaagccgg actcatccag
     1621 cagcaggctg atgataatga cggcaataaa cgccgccgtc gcgttccaga cgatattcca
     1681 caccaccgga atatcacccg gatgaaccac gcccgtaacc aacgccagta ccgcgccgag
     1741 cgttgcactc cagccgatgc ctaaaccttt cggctgccag ataaccaata cgatggtcag
     1801 gacaaagata gcgcctgcca gtaacataat gcctcccgga taaaacacat gtgcaaatcc
     1861 atatgtgttt agctaaattt ttaactgcaa atgttcttac tgtctccgga acagttttgt
     1921 ctggccaggt tgcggacaat tgcctgaatc ttttcctgtt cacatcgcca ggcttcatca
     1981 ataattttcg ccgcccatgc tggaatatgc ggcgataagc ggtaatgaac ccacttacct
     2041 tgcttgcggt ccagcaatag cccgctttca cgcagcaatg ccagatggcg ggatatcttg
     2101 ggctgcgact gatcgagggc agtgcagaga tcgcagacgc ataactctcc cagttcgcta
     2161 agcagtaaaa cgatgcccag acgggtttca tcagcaagaa ttttgaacaa ttggatggg
//
LOCUS       DAJTSI010000295         2209 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000295 DAJTSI010000000
VERSION     DAJTSI010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.301"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..104)
                     /locus_tag="OO327_004157"
     CDS             complement(<1..104)
                     /locus_tag="OO327_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HCR6020476.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
     gene            complement(145..600)
                     /gene="csgB"
                     /locus_tag="OO327_004158"
     CDS             complement(145..600)
                     /gene="csgB"
                     /locus_tag="OO327_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR6020477.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAVVVQRQSQMAIRVTQR"
     gene            1354..2004
                     /gene="csgD"
                     /locus_tag="OO327_004159"
     CDS             1354..2004
                     /gene="csgD"
                     /locus_tag="OO327_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR6020478.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            2009..>2209
                     /locus_tag="OO327_004160"
     CDS             2009..>2209
                     /locus_tag="OO327_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="chaperone-like protein that participates in the
                     polymerization of curlin (CsgA) subunits into curli
                     (extracellular fibers from Escherichia and Salmonella spp.
                     that are involved in the colonization of inert surfaces
                     and biofilm formation); part of the curli secretion and
                     assembly protein complex; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgE"
                     /protein_id="HCR6020479.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSA"
BASE COUNT          643 a          468 c          413 g          685 t
ORIGIN      
        1 ccaccaccgt ggttaccgcc gccgccgtac tgaggaacaa cacctgccag agcgctaccg
       61 gagaatacga ttgctgcaat tgctgctact tttaaaagtt tcatgtaaaa cccccatcgg
      121 attgatttaa aagtcgaatg gtaattaacg ttgtgtcacg cgaattgcca tttgcgactg
      181 tctctgcact acaactgccg ttttttgagt accatactgt gtaatatttg ctttattacc
      241 agaacctttc tggataatca tcgcagtatt accataagca ccttgcgaaa tactggcatc
      301 gttggcactg cccgcctgat caatatatgc aaggttataa tctcctgtct ggtcaatctt
      361 tgcccgattg ctactacctt cttgcgcaac aaccgccaaa agttttgagc ctccctgccg
      421 taactgagca ctattattag tcccagcctg accaattatg gctgcctgat taaatgaaga
      481 cttactcaat tcatttaccg cgaagttata ttctgaatta gctaaatcat aacctgctgc
      541 ggctgcaatc ccaggcgcac ccagtattgt taacatcata aataacaatt tgtttttcat
      601 gttgtcaccc tggacctggt cgtacattta agaaattaaa tcatttcaac ttggttgtta
      661 acgcaacctg tattttgtta acgctgcgtt acgatggaaa gtatgtctgc ggaaatattt
      721 ttaataactc acccgcgcgt tgtatttttc tttttttgtt cataccaaag tattaaaacc
      781 cgcgtaaata cgctgttatc tacgcaaaaa atattttgtt ttctcttaaa tctccgtttt
      841 ccgctatcaa aaatcaccag acagtcattc ttcttgcccg tcgctgattg ctgcgatatg
      901 tcttgcgcac aagccgtgac aaacctcgtc tttttgcagt aaaaaaattg tccacggagg
      961 tgtggagaaa aaacaaaaac gttttacatg acgaaaggac tacaccgaaa tattttttat
     1021 atgtattatt agtaagttag caccatttgt atgatttttt aaaattgtgc aataaaaacc
     1081 aaatgtacaa cttttctatc atttctaaac ttaataaaac cttaatgtta acattttaat
     1141 ataatgagtt acattttgtt acatgtttaa cacttgattt aagatttgta atggctagat
     1201 tgaaatcaga tgtaatccat tagttttata ttttacccat ttagggctga tttattacta
     1261 cacacagcag tgcaacatct gtcagtactt ctggtgcttc tattttagag gcagctgtca
     1321 ggtgtgcgat caataaaaaa gtggggtttc atcatgttta atgaagtcca tagtattcat
     1381 ggtcatacat tattgttgat cactaaacct tctttgcagg cgacagctct cttgcagcac
     1441 cttaaacaat cgctagcaat tacaggaaaa ttacataata ttcaacgttc tctggacgat
     1501 atctcttcag gctctattat tcttctggat atgatggaag cggataaaaa gcttatccat
     1561 tattggcagg atactttgag caggaaaaac aacaatatca aaatattgtt gctaaatacg
     1621 cctgaagatt acccgtaccg cgacattgaa aactggcctc atatcaacgg cgttttttat
     1681 gccatggagg atcaagaacg tgttgtcaat gggttgcaag gcgtcctgcg tggcgaatgc
     1741 tactttacgc aaaagcttgc cagttacctg attacacatt ctggtaacta tcgttataac
     1801 agcacggaat cagccctcct tactcatcgg gaaaaagaga tcctgaataa gctgcgtatc
     1861 ggcgcgtcta ataacgagat cgctcgttcg ttgttcatca gcgaaaatac ggttaaaacg
     1921 catctttata atcttttcaa gaagatagcc gtaaaaaacc ggacacaagc ggtttcctgg
     1981 gcaaacgata acctcaggcg ataaagccat gaaacgttat ttacgctgga ttgtggcggc
     2041 agaatttctg ttcgccgcag ggaatcttca cgccgttgag gtagaagtcc cgggattgtt
     2101 aactgaccat actgtttcat ctattggcca tgatttttac cgagccttta gtgataaatg
     2161 ggaaagtgac tatacgggta acttaacgat taatgaaagg cccagtgca
//
LOCUS       DAJTSI010000296         2183 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000296 DAJTSI010000000
VERSION     DAJTSI010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.302"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..694
                     /locus_tag="OO327_004161"
                     /pseudo
     CDS             1..694
                     /locus_tag="OO327_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            696..1301
                     /locus_tag="OO327_004162"
     CDS             696..1301
                     /locus_tag="OO327_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCR6020480.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1288..>2183
                     /locus_tag="OO327_004163"
     CDS             1288..>2183
                     /locus_tag="OO327_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6020481.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRV"
BASE COUNT          531 a          507 c          609 g          536 t
ORIGIN      
        1 cccgtgccgg tgtgccgggc agcgatgagg aaacccgtgc ggactttgaa aaccgccgac
       61 gtaattcagt tgcccgtaat gcccggaata ttctggaagc catccggggt gaaatactct
      121 ccacggtaga aaacgtggtg gatgtttacg tcacccataa tccgaaaaaa acagaacaaa
      181 aagccggagt cagtatccat taacacccgg ttcgctttat gttggcgtgt acggcggcag
      241 cccggcagat atcgcggcgg ccatctggcg taaggctccg ccgggtattg atatgaatgg
      301 caacacaacg ttcactgttg cagataagga gtacgatccg ccgtatcctg aatacgtgat
      361 cagctggcag acactcaaac ccgtcagtct gcatgtcagt gtgacgctga aaaaaagtga
      421 ctacctgcct tcagatatta cccgacaggt acagcaatcc gtgctggacg catttaatgg
      481 tacagatggt ggtctgcggg caagagttgc ctctgttgtc tctgccgggc gctactatgc
      541 cggcatttac aaaaccgatc cggaacatat tgatattctg ggccttactg tgagccgtga
      601 tggttcgtca tggacaacgg ctgtcacttt cgggatagat gagattccgg ttctggatgt
      661 gtcggatatc agtgtgaaac tccaggaggc ataacgtgca gaatgtggct gccactgtgc
      721 ttgcgcagta tgctgccagc ccccgactca atgccctcat taacagcttt aacgcagcgc
      781 tttcccccga cagttttatc agtgattttt atggtcttat ctggaacatc gataccgcag
      841 aaaagtatgg tcttgatgtc tggggaaaga ttgtgggtgt cagtcgctgg ctgacggtaa
      901 aggacgattt taattacctg ggcttcagcg agtccaggat ggacaccccg gtaatggatg
      961 atccctgtcc gtttaatcag gcaccgtttt acaacggaaa atcggatacc cggactgttg
     1021 acctgtctga tgctgtatac cggcggctga tactgatgaa agccatgtcg aacattactg
     1081 actgttccgt tccggatatt aacctgatgc tgagatttat gttcggaaaa aaacgccggg
     1141 cttatgttct gaataatggc gggctgagga tgagttacgt ctttgagtcc gcgctctcgt
     1201 tggcagaact ggcgattatc cagtcgtcgg gtgcactgcc atccccgccg ggtgtttatg
     1261 tttcagtagt tttaaaggag tcccgtaatg aaggccagtg ataaaccccg ccagctggcg
     1321 gtcccctttg cgagtaccgg agataaaaac cgtatcccgg acaaggcgac acagcagacc
     1381 agagagagcg gtaatgctgc gtatgattca ggttttcctc cggtgaccat gacagcggtc
     1441 tcagcgggag gtataccgcc acacggcaag gattttaacg gtctgatgta cgatattacc
     1501 gcagcaatac ggttcgccca ggctggcggt ttgtacacgt ataatgccgg ttttgcgggg
     1561 gccattggtg gatatgcaaa aggagccatt ctcgccggag tcgcaacaac agcggtctgg
     1621 ctgaatacca cggacgataa cctgaccgat cctgaaggct ccgacagtgc gggctgggta
     1681 aatcttcttg aggatccgaa aaggatattc ctgcggcaga agaacaatct gtcagacctt
     1741 cagaataaag ggacggcacg ggataatctt caggtttaca gtaaagagca gtcagatcaa
     1801 cgctatgttc atcgggaagg cgataaaata accggagagc tgaaaatccg tggtgttaat
     1861 gcgctgagga ttttcaacga agcttttggc ctgatttttc gtcgttcgga agagtgcctg
     1921 caccttattc ccaccagtga aggtcagggg gaaaatggcg atattggtcc cctgcgcccg
     1981 ttcaccatta atttgcggac gggtgaaata tccatgtcgc ataaagtgtc tgttggcggt
     2041 ggttcgcagg tcaatggtgc gctgggtatc ggcgttcaga acgccctggg ggggaattca
     2101 attgttcttg gtgataatga caccggattt aaacagaatg gagacggtat tctggatgtt
     2161 tatgctaata gtcagcgtgt att
//
LOCUS       DAJTSI010000297         2182 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000297 DAJTSI010000000
VERSION     DAJTSI010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.303"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(28..676)
                     /locus_tag="OO327_004164"
                     /pseudo
     CDS             complement(28..676)
                     /locus_tag="OO327_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            741..1892
                     /locus_tag="OO327_004165"
                     /pseudo
     CDS             741..1892
                     /locus_tag="OO327_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1922..2182)
                     /locus_tag="OO327_004166"
                     /pseudo
     CDS             complement(1922..2182)
                     /locus_tag="OO327_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          604 a          482 c          547 g          549 t
ORIGIN      
        1 gtagatacat acgcagtcag agtacactta ctttttatta tttcctgcaa tggcgattac
       61 ggctattgac agtaatgaag ttattagtga accaagacct aaagtagttc cgggaccaac
      121 accgttgtcg cttacggtta ctgacaggaa actgattgta ccagcgctta ttgtttgagt
      181 aaaacctgta atagatgcgg caaatcctgt ttttgaacca tttatttgta atgcgataaa
      241 attggctatt gggtaataaa aaccataggt tggataattt ttcaatcttt gataattgat
      301 ttccgattat gaaaaataaa actgagaagg agaaaataat tgagtaacta aaagtactca
      361 ggccataagt atgcatgaaa attgatggtg atattgatat gtagcaaaat agagaactga
      421 gtattaatga gacaagtata gtgccgcaga taaactcttt ggtcacaaag gatgcactgt
      481 tttgacatat tactttctca tttgcatgag ttatctttgg tgataataac attattataa
      541 tactgaacag catcataaca agaaatataa cattccagtt ataatattca ataatcaatg
      601 cgcgaggtgc tactaatggt attactgcgc ttacagatga tatatcagaa agcgcccgtt
      661 ttagttttaa tggagggtga tcttacccac caatagtgga cacaggacta agtgagtaaa
      721 ctctcaacca gaggtgactc atgacaaaac cagtatcaat cagcaagaag ccccgtaaac
      781 aacatacgcc tgaatttcgt aacgaagccc tgaaactcgc tgaacgcatc ggtgtggccg
      841 ccgcagcccg tgaactcagc ctgtatgaat ctcagcttta tgcctggcgc agtaaacagc
      901 agcaacaaat gagttcgtca gagcgcgaaa gcgaactggc cgctgaaaat gtccgcctta
      961 aacgacaact ggcggagcag gctgaggaac tgtccatcct ccaaaaggcc gcgacatact
     1021 tcgcgaagcg cctgaaatga agtatgtctt tatcgaaaat catcgggcag agttcagcat
     1081 caaagcgatg tgtcgtgtac ttcgggttgc ccgcagcggc tggtatgtct ggctcaggcg
     1141 tcgtcaccag atgagcctgc gccaacagtt tcggctcacc tgcgatgccg ctgtccataa
     1201 ggcattcttt gaggcaaagc agcgatacgg tgctccccgc cttgctgacg aactgccgga
     1261 gttcaatatt aaaaccattg ccgccagcct gcgtcgtcag gggctgcggg cgaaagccag
     1321 ccggaagttc agcccggtca gctaccgtgc acatggcctg cccgtattgg agaatctgct
     1381 ggagcaggac ttcagcgcca gtggcccgaa accagaagtg ggcgggtgac atcacgtact
     1441 tgcgtaccga tgagggctgg ttgtatctcg cagtagtcat cgacctgtgg tcacgcgccg
     1501 tcattggctg gtcgatgtca ccgcgaatga cagcacaact ggcctgtgat gcactgcaaa
     1561 tggcgttgtg gcggagaaga cgcccggaaa gcgtcattgt tcatacggac cgtggtggtc
     1621 aatactgttc aggggattat caggcaatgc tgaagcgaca caacctgcgt ggcagtatga
     1681 gtgcgaaagg ctactgttat gacaatgcct gtgtggaaag cttctttcat tcgctgaagg
     1741 tggaatgtat ccacggggaa cgctttagca gccgggaaat aatgcgggca acggtgttta
     1801 attatatcga gtgtgattac aatcgctggc gtcgtcacag tgcctgtggc ggtctcagcc
     1861 cggaacaatt tgaaaaccat aatctcgctt agggccgtgt ccacattacg tgggtaggat
     1921 cagtatgggt cgtcttgtat ttgtcatact gcccaatctc accaatcgaa aatgctcctt
     1981 ttggcaaagg ttcaagcagg tctctgggaa tggaggtcgc tggcgtcgga tataaaaaac
     2041 cctcacggct tagcaaggct ggcgtcacta tcggtgtaac aatatcaggc gtgcctgcgg
     2101 aacggtcctt accatgctgc agtaaccgga aataaacttt tgggcgtaag atgacattga
     2161 cagtttcgac atcgtctttt tc
//
LOCUS       DAJTSI010000298         2168 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000298 DAJTSI010000000
VERSION     DAJTSI010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.304"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..576)
                     /locus_tag="OO327_004167"
     CDS             complement(<1..576)
                     /locus_tag="OO327_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR6020482.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETG"
     gene            complement(628..2118)
                     /locus_tag="OO327_004168"
     CDS             complement(628..2118)
                     /locus_tag="OO327_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR6020483.1"
                     /translation="MDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKS
                     TPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETF
                     LPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDY
                     FMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAY
                     NAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTII
                     LFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDF
                     YPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWD
                     NYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDN
                     LAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
BASE COUNT          574 a          442 c          488 g          664 t
ORIGIN      
        1 cccggtttct agcaattcga taaattgcat atgtttactg ccgatagatt ttaaaaaatg
       61 ataaacctca agaggataat ggacattaac gttattaatg acggttaacg tattaaactc
      121 tacttgatat gatttcagac gctcgatggc tgctatcact tttgcaaaag taccgttacc
      181 tgaattactg cgtctgtaac ggtcatgtaa ctcctggggg ccatcgatcg agataccaac
      241 cagaaattca tgttctttga gaaaggcaca ccattcatta ttcaataaaa tgccattcgt
      301 ttgtaatgta ttaaaaatac gtttttggcc tgcatagcgt tgttgatagt gaataacttt
      361 acggaaaaaa tccaggccag ccagagtggg ttcaccgcct tgccaggtaa aatagacctg
      421 attgccagac gcttcgatat attgtttgat gaactctttc agagtgctgt catccatcca
      481 tttttcatga gtaaactgcg actctttttc aaggtaaaaa cagtaatcac atttgagatt
      541 acattgaaaa ctggagggct tggctgtaac gtgcatcgct atctcgctca ataaggcggc
      601 ggaaaaatcc gccgcatgaa ggtttagtta tttcgcttcg cttagtgctt tcttgatatt
      661 gttaaacttc tcctgattta cctcgctaag cggtggctgg ctgctgtcga taaactctct
      721 taccacgcct tgcatctctt taacgacctg cggattggcg gcggcaaggt tatctttttg
      781 ctgtagatcc gtcagtttgt agagacctaa ctgattgttt tctactgtat agacaagcga
      841 ataatcgtta tttctcaccg tataagagaa ttggcttaag tcctcagtgt tggggttatg
      901 cgggtaatcg tctgactgat ggcgaacaaa tttgtggtaa ttatcccaga atggaatatt
      961 ttcctcatca aaccagtgag aataagaggt tatccaggtc agatttttat gtggctcgcc
     1021 ttgtttctta tcttgcaacc agggcagcaa gggaacacca tccagcttaa ggtcttttgg
     1081 aatgctgata tcggctgcat caagagctgt tgggtagaaa tccattgcgg aaatcagctt
     1141 gtcataatta ccgggttgaa gttttccttt ccaccacata aacattgggg tgtgagtacc
     1201 gccaggatag gtctgactct tatagccttt ttgcgccccg ttcagcggca gaggaccatc
     1261 gataaccgca ccattatcgg aggtaaagag aataattgta ttgtcatact gtccgttttt
     1321 cttcagttgt tcgagaatgc gttttacacc ctgatcaaca gaataaacgg aagcgtagta
     1381 gttatctgct gtttgactac cggtattaaa ttgcttctga tattgatccg gtgcaggatt
     1441 atcatttggt aggtgcggag cattataagc caggtaaagc ataaaaggct ggtcaagtgt
     1501 tttggcacga tcaacaacgc caattgcctc atcggttaac tgatcgctga tataaccttt
     1561 tgcggggaca cgttcacgat ttttgaacag tgaaggggag ttgtaatatg ccgttcctgc
     1621 agcgtggaat cccataaagt aatcaaagcc acggttttga ggttgccatt cttccgcaga
     1681 aaatgtggtg aagttgtcat gatagtcacg cgtttgttta tcttccggta ccggcacatt
     1741 actgattttt gacaagtgcc atttacctac tgctgcagtg taataaccat gattctggaa
     1801 taattcaggc aagaaagttt ctgttagcgg aataccatcc tgagcatcgg tattggaata
     1861 gacaccaaag cgggcgggag ctcgaccggt cattattgcg gcacgggagg ggccggaaac
     1921 accgtgtgcc acatagccgt tagtaaaacg tacgccttca tccattaatg aaaggagcgt
     1981 cggcgttgat ttttgtgcag cttcaatggc tttatctatc cctattttgt aggtatcgac
     2041 aacttcacga ttttccattg tttttgggtc aaaagatccc ttatcaaaag gaagttgtcc
     2101 ataaccaaga tcatccatgg ttagtacgat aatatttggc tttcctttgg tactgtattc
     2161 agtcggcg
//
LOCUS       DAJTSI010000299         2162 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000299 DAJTSI010000000
VERSION     DAJTSI010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.305"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            80..259
                     /locus_tag="OO327_004169"
     CDS             80..259
                     /locus_tag="OO327_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020484.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            311..505
                     /locus_tag="OO327_004170"
     CDS             311..505
                     /locus_tag="OO327_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020485.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(1304..1640)
                     /locus_tag="OO327_004171"
                     /pseudo
     CDS             complement(1304..1640)
                     /locus_tag="OO327_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          688 a          444 c          450 g          580 t
ORIGIN      
        1 atactggttg tctggagatt cagggggcca gtctaggtag cgtaaggcac tacagcgcat
       61 tgctttacga gtaataacaa tggatttatc accagaagac gcaaagtttt tacgagaact
      121 gcaacaccgt atgctcagag aagccattat cgaacagcaa acgggcatga gcgccataga
      181 cccattgcga tacaaacggg cctcatctcc tttagttgac gacaattcca atgcggataa
      241 aacacgtcca ccacactgat tacatttttc attagcacag tggtgtgatg cagcatacga
      301 ggaaaaccgg atgaatgaga acaaaataaa aaggctggag caactgttac aggcacgcca
      361 acgggagttt gccactaaat ggggacgtgc cgcgctgcca catgaacgcc taacctattt
      421 tccacttgaa gacctcatta aaaataatgg caaaaaaatc aggtctgata agaaacactt
      481 cttagaatgc aatgaagaca aataacaaca atctgcatat tgccaccaac cacagatgtg
      541 gcatcgttat cagcgcactt gattttcttt atattcactg acgagcttgc cggggcacct
      601 ttgtttgccg gtcattctgg tatcatgcag atatttacgg cgtacaagcc cactagcatg
      661 acattgtaat aaggcctgag cctaacgctt taatgtgtgt catacactca gtggcgtaat
      721 cgttgacaat atggtgcatg taatcgtaag cgtccagcga aatccatctg gtcataatct
      781 gaagcatccg acaaagtggt gtccaccaaa taagtgatct gcgcccaata tcctgagccg
      841 gaactttgac aaaggccaac gaaaaatggg agcggatgtt acagagttct cggtacaggg
      901 gaaaagtggt acctgtcgcc agttctccga tctgtttaac cgggaaatta tctcctacag
      961 cttgtcagaa aggtcggtga tggagcatcg ttaatacgat gctatacgat gcattcacag
     1021 tgcccggcca gaggatgccc cgtcgctgca tatggatcag ggttggcaat atcgaatcgc
     1081 aggctatagg caaagttaaa gccccatgga gtagcacaaa atatgccgca caaaggaaac
     1141 ggtctgaata acgcagtgat gaagaacttc ttcagcacac tgctaaaacg catagtgatc
     1201 gagcgctgat tctggcgaac aactgaacta atacatcaga atctgcatta tgttaaataa
     1261 atatgaaaag atggtttaaa taccccgtta cttttgactt acactatacg gtatcgcatc
     1321 gtttaatatt cgcaccggcc agatttttat ttctattagt tgtcacaata ctgaatgcgt
     1381 acgaccacag tattctggct cctgtgtggt tatgctttaa ttctgcgttc cgggcagata
     1441 agcagttgct tgcaggaatc cttcttgtgt taatgtcagt tccccttttt accagtgctg
     1501 atttccacat tccgtccaac agagcttata gcctttccct ggattatagc attgtccggc
     1561 tgaagttctt tttgaataat aatagaagca ctgctggcag atccagtccg tttttcataa
     1621 cccactgtac tgataaccat aatctaatca gtagaaattg agtcgaaaat aagcactact
     1681 ctatacagga taattagagg tcagtttgat tattcacaat tcatcatcag cattttctat
     1741 ttctgacgaa atcaacatga aaataaccat atatgataat tattataata acggctttaa
     1801 ttggaataca tatattacaa cgtattatat ataattggta ttctgggaac tatattctca
     1861 aaatacagta gaaacgaggt atgtttctgg tggaaaggac agtgggatta aaaagtaaga
     1921 attgataaaa aaaacgccag caacacatag tgccggcgga gggaataccc catggagaaa
     1981 atgtgatgcc tagagcatca tgaatatata attaaaaata gttagcgttg tcaatacttt
     2041 cacaaaaata attttcgtag tatagaaaga tatttttatg catgacctac ctgaatttgc
     2101 tccgggtaga ggttataaat aaaaattgaa tcacgacaaa cacaatattc atagtatggc
     2161 ga
//
LOCUS       DAJTSI010000300         2153 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000300 DAJTSI010000000
VERSION     DAJTSI010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.306"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1313
                     /gene="argS"
                     /locus_tag="OO327_004172"
     CDS             <1..1313
                     /gene="argS"
                     /locus_tag="OO327_004172"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR6020486.1"
                     /translation="AVRTLEFLGHKVIRANHVGDWGTQFGMLIAWLEKQQQENAGEME
                     LADLEGFYRDAKKHYDEDEEFAERARNYVVKLQSGDEYFREMWRKLVDITMTQNQITY
                     DRLNVTLTRDDVMGESLYNPMLPGIVADLKAKGLAVESEGATVVFLDVFKNKEGEPMG
                     VIIQKKDGGYLYTTTDIACAKYRYETLHADRVLYYIDSRQHQHLMQAWAIVRKAGYVP
                     ESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLADLLDEALERARRLVAEKNPDMPADEL
                     EKLANAVGIGAVKYADLSKNRTTDYIFDWDNMLAFEGNTAPYMQYAYTRVLSVFRKAE
                     INEEQLAAAPVIIREDREAQLAARLLQFEETLTVVAREGTPHVMCAYLYDLAGLFSGF
                     YEHCPILSAENEEVRNSRLKLAQLTAKTLKLGLDTLGIETVERM"
     gene            1490..1978
                     /locus_tag="OO327_004173"
     CDS             1490..1978
                     /locus_tag="OO327_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCR6020487.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
BASE COUNT          611 a          492 c          535 g          515 t
ORIGIN      
        1 cagcagtgcg tactctggag ttcctcggtc acaaagtgat tcgcgcaaac cacgtcggcg
       61 actggggcac tcagttcggt atgctgattg catggctgga aaagcagcag caggaaaacg
      121 ccggtgaaat ggagctggct gaccttgaag gtttctaccg cgatgcgaaa aagcattacg
      181 acgaagatga agagtttgcc gagcgtgcac gtaactacgt ggtaaaactg caaagcggtg
      241 acgaatattt ccgcgagatg tggcgcaaac tggtcgacat caccatgacg cagaaccaga
      301 tcacttacga tcgtctcaac gtgacgctga cccgtgatga cgtgatgggc gaaagcctct
      361 acaacccaat gctgccagga attgtggcgg atctcaaagc caaaggtctg gcagtagaaa
      421 gcgaaggggc gaccgtcgta ttccttgatg tgtttaaaaa caaggaaggc gaaccgatgg
      481 gcgtgatcat tcagaagaaa gatggcggct atctctacac caccactgat atcgcctgtg
      541 cgaaatatcg ttatgaaaca ctgcatgccg atcgcgtgct gtattacatc gactcccgtc
      601 agcatcaaca cctgatgcag gcatgggcga tcgtccgtaa agcaggctat gtaccggaat
      661 ccgtaccgct ggaacaccac atgttcggca tgatgctggg taaagacggc aaaccgttca
      721 aaacccgcgc gggtggtacc gtgaaactgg cagacctgct ggatgaagcc ctggagcgtg
      781 cacgtcgtct ggtggcagaa aagaacccgg atatgccagc tgacgagctg gaaaaactgg
      841 ctaacgcggt tggtattggt gcggtgaaat atgcggatct ctccaaaaac cgcaccacgg
      901 actacatctt cgactgggac aacatgctgg cgtttgaggg taataccgcg ccatacatgc
      961 agtatgctta cacgcgtgta ttgtccgtgt tccgtaaagc agaaattaac gaagagcaac
     1021 tggctgcagc tccggtaatc atccgtgaag atcgtgaagc gcaactggca gctcgcctgc
     1081 tgcagtttga agaaaccctc accgtggttg cccgtgaagg cacgccgcat gtaatgtgtg
     1141 cttacctgta cgatctggcc ggtctgttct ctggcttcta cgagcactgc ccgatcctca
     1201 gcgcagaaaa cgaagaagtg cgtaacagcc gtctgaaact ggcacaactg acagcgaaga
     1261 cgctgaagct gggtctggat acgctgggta ttgagactgt agagcgtatg taatcgattt
     1321 ttcgtgagag tgaagcctga tcagggttag ccgatcaggc ttttttattg ccatctaaat
     1381 gtattctgaa aacggacatg ccattgtttt ctcactgttg agtaagaggc cagaagcgta
     1441 atatccaggc ccaggaaaac gataacggtt caatttaagg aataccgaag tgtttaaatt
     1501 tcttgtatta acattaggca ttatctcttg ccaggcttac gcagaagata cggttatagt
     1561 aaacgaccat gacacttcag ccatcaaaga ttgttggcaa aaaaattcag atgatgatac
     1621 tgacattaac gtgatcaaat catgcctgcg acaagaatac aatctcgtcg atgcgcaatt
     1681 aaataaagcc tatggtgaag cttatcgtta tatagaacaa gtgccacgca caggtgcaaa
     1741 aaaacttgat actgaacaac ttaacttgct taaaaaatca cagcgagcct ggctggattt
     1801 tagggacaaa gaatgtgaat taatcctttc aaatgaggat gttcaggatt taagtgaccc
     1861 ctattctgaa tcagaatggc tatcatgtat gatcatacag accaatacgc gaactcgcca
     1921 gttgcagcta taccgtaact ctgaagattt ttatccaagc cctttgacaa gaggataatt
     1981 cacatctttt tggcatgttt tgttgcaagc tattcctgat aaatgattgc aacaaaacat
     2041 ccagcctttt gactgagtta ttaaacatac tcgcgagcgc gtaatttttt gtctttcagc
     2101 gataattcac aatcacttca ttccgttgta cctttagcgg tggaattaac cgc
//
LOCUS       DAJTSI010000301         2151 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000301 DAJTSI010000000
VERSION     DAJTSI010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.307"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="OO327_004174"
     CDS             complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="OO327_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCR6020488.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
     gene            1096..1275
                     /locus_tag="OO327_004175"
                     /pseudo
     CDS             1096..1275
                     /locus_tag="OO327_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1292..1982)
                     /locus_tag="OO327_004176"
                     /pseudo
     CDS             complement(1292..1982)
                     /locus_tag="OO327_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          630 a          398 c          493 g          630 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gcgagtcgac
       61 aatggattcc ctgaaatatt aatcctgtaa cttgaatcta attgtgaaaa actctccgga
      121 agagtggtta tctgattaaa atgaaaataa tattcctttt gtctgatatt gtttctatca
      181 gaaaattgag gaaaagtaac cagattattg cgctgagcac agagaatttc cagtttatcc
      241 ggtaagtcgg gtaatgttgt cagcctgtta taataaacat gtaaaaattt caatttttcg
      301 ggaagagccg gtaagttata tagcctgtta ttttcaacaa ataatgatgt taaatggttt
      361 ggcaaaacag gaaggttttc cagttgatta gaacgaacac ttaaagttgt taatgactca
      421 ggaagttctg gtatcacaga aagcctgtta ttatcagcat taagttcttt aagaaatgca
      481 ggcagctttg gcaatgatgt taatttatta ttttctacaa tcaaagcaga aatatgcttt
      541 ggtaattcgg gaagagacgt caaatttagg tctgataaat ttaatactgc ttcttgattc
      601 tgaaagcact gggagagtct gttaaaagca atgcctcgct cttccccggg agaagagttc
      661 ttttcccatt cagtcaggat tctcagataa tgttcattac tggttgagtt tgagttaata
      721 gagggggagc aagaaactga tgagtgtgta ttatttacag agaacataat gctaggtcaa
      781 ccttatattt gtgagaataa atatttaaaa ttgtatattt atacaaattt cacttaccat
      841 ttccgatatt aagttttaat agtttccatt ttacacataa cagaagttag ttgtaattta
      901 tttatggagg ttttctatgg aattcttttt ttattgtggc tgcagtcaat aataacactg
      961 aattcaacat tccagtgagc aaggaaatgt aagccgagta agtatatttg ttttacatca
     1021 tattttagtc aagctcattt attcctatta tagaatgaca aattaaaatt actgtaaaaa
     1081 aacgatacac atttaatacg agggaggccg gcagaaaaac ggcttgcggt caggcaggag
     1141 aaatgtgtgc cgcgaatgaa gtccttgtat gactggataa agctccagag gaaaactttg
     1201 tcaaaccatg ctgaaatggt aaaggcgttc aactatatcc tgaatcactg gaatgcactg
     1261 aatgagtttt gccgggtagt acttccaatc attagaatat gtgcttttca atgaaggaac
     1321 cgatgacttt ttcgtggatt tccacggaac gcgagaagca gattgtttta cgtgccagcc
     1381 gcttgatgcg ggggcggagg gtcaggttgt tacgttcata cgttgcgtga agattttgcc
     1441 ggtcaggtgt ttctcctccg gcacttctct ggcgtagctg ccccagtcat cgctggtcac
     1501 catgccgata cagaacggag tcaacagggc taacagttcc cgacaggtct catcattccg
     1561 tggcccgcaa gtgtaagcca acacaccgcc agttttagtg ttgtacgcat accagggcca
     1621 gtgttgttgc gccttgctgc caacgaagcg ccactgctca tcaagttcac agataagggc
     1681 aacatcagcg tgagcgactg gcgcagatgt tatccgccgg ggcgagagct ttttaatgtc
     1741 cgtatgacgg tattgatgcc gattttcagt gtcctggcgg tatcacggcc ccctgcgccg
     1801 ttatgtgcca tttcaacgat ctgctcctta acgccgagtt ttctggcttc ataagagtaa
     1861 gtgagctgga aaacacgttt acatgagcga caacggaaac gctcatgccg ggaacaacta
     1921 cggccatgtc gataaacttc atcggaatta cagcgcggac aatgaacagt aacggtagtc
     1981 atggaaaacc tcaaatacca gtagattacc agaaacttct atcgattgaa gacattacct
     2041 tttgccgcaa taggtggggt aacattggtg aaaacgcatt acgccgggtg gcagttggaa
     2101 aaaaattatc ttttttcggc tctgatcgtg gtggagaaaa tgcggcaata a
//
LOCUS       DAJTSI010000302         2111 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000302 DAJTSI010000000
VERSION     DAJTSI010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.309"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..157)
                     /locus_tag="OO327_004177"
     CDS             complement(<1..157)
                     /locus_tag="OO327_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020489.1"
                     /translation="MRKLKMMLFGASLIMVVGCSSKENALCHPQPKPPAPPAWAMMPP
                     SNSLQLLD"
     gene            complement(374..907)
                     /locus_tag="OO327_004178"
     CDS             complement(374..907)
                     /locus_tag="OO327_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR6020490.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(973..1602)
                     /locus_tag="OO327_004179"
     CDS             complement(973..1602)
                     /locus_tag="OO327_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020491.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(1606..1821)
                     /locus_tag="OO327_004180"
     CDS             complement(1606..1821)
                     /locus_tag="OO327_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR6020492.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          540 a          561 c          465 g          545 t
ORIGIN      
        1 cgtccagcaa ctggagagag ttcgacggtg gcatcattgc ccatgccgga ggcgctggag
       61 gtttcggctg aggatggcac agggcatttt cctttgacga gcacccgacc accattatca
      121 agcttgcgcc gaagagcatc attttcagct ttcgcatcag ctaactcctt cgtgtattta
      181 gcatcgagtg catcagcatc acgctggcgc tgctgcatgt cagtaatggt tgcgttcgcc
      241 agcttcagtt ctccggcgtt tttgtcgcgc tgctctttgt aggtaatggc gttatcacgg
      301 taatgattaa cagcccatga caggcagacg atgatgcaga taaccagagc ggagataatc
      361 gcggtgactc tgctcattga tctatccccc aacaggctaa tgcgctttcc tggtcacgac
      421 gaataacctg tccatagcag ttatttgaac gtatgcggca atcgcgccca acatctttta
      481 tccaccagcg aatcgcctcg catgcgccct tacgatcacc ggcattcagc cgcttataaa
      541 acgtcgacgg gaaacactta ccggggccaa tgttataggg gcaaaatgac gctatacccg
      601 ctttctgggg ttcggtcaga ggtactttaa tattgcgctc cacccatgcc agcgccttat
      661 cacgttcaat agcgttaacc tggtcacatt tttccttcga cagcttcatt cccggtatga
      721 caggcttacc atccaccatt gtggcaccac gacagatggt ccatatgcca gaaccatcac
      781 ggtatgccgt tgtgtgatta ccctcttttt cgttcagaaa ctggtcaagt atctggggag
      841 cagacgcgcc tgcggcaatc agcgccagaa cagcagccga caggccgtat ttgatttttg
      901 cgctcatgga tatttatcag gatttatcgg caacaaataa cgagccaact tatatacgcc
      961 ttttaagata agtcagcccc ggctggatcc agtcatcagg ctctttctta aagggggagt
     1021 attgaaatca cgaagaagag cctcccgcac cgcagcatcc atgtctgcac cactggcaag
     1081 tgcttcaatc tctgccgcca cctgcaaata ccccatgcaa cggccaatgc gtttcataac
     1141 tctggtgttt tcgtctttca ggcatctgat agcgagatta ataattttca ggcagtgatg
     1201 atgcgatgcc cggacaggtt ttccgttctc ataaacgacg aactcaccct cttcacagga
     1261 aaccctgaaa ggccgatggg gttcctggtc tttatccggt gcagacgcaa taaaatcact
     1321 tttatgctct tcctctccgg cactgttaac cgtaatgacg tactcaggcc gcaggtaagc
     1381 cgcctctttt tctgacaatg cggccggtaa aaacttttcc tgcaaaaact tcataaaagt
     1441 ctcgtgagcg actttgtggc agtaatcgta aatcgccgca taatgctcat cgcggcgttt
     1501 gttttcatct tcagaactca ccaacgccga caatttttta ttaagctcag tgatttcatt
     1561 ttccaggtga ttgaatcgct gattcatttc ttcatgcttc attatttact ctccccgtgc
     1621 agccttacgc ttgtcttctc ttattttgaa atacagattc gtcagataag tcagaagccc
     1681 cagaagcaga cttcccagca caccaatcgc agcccactgt gatggcctga cctgatccaa
     1741 ccactgcaaa aaccagtagc cggcactgcc ggcggaggtg ccgtaggcaa tgcccgttga
     1801 aattttgtcc atggatttca tagcctcacc tccgcaaata acggatggcg tagttttaca
     1861 ctgagaaatg aaagggattt gaaaagaaaa aaacgcaaaa gcgggcgaaa cgatatatac
     1921 agtaaggaaa gcactctatc caacaaacca cccacagtta atcggaataa aagcagagtg
     1981 cttatgaatg atcgcacgcc cgaaggttag tatttctgca cagcaatttt gcaaaaaaag
     2041 gcgatcattc ataacttaaa cgtctttcag tcactccggg atttcccatc atcgcagact
     2101 gaaagactct g
//
LOCUS       DAJTSI010000303         2084 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000303 DAJTSI010000000
VERSION     DAJTSI010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.310"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..796
                     /locus_tag="OO327_004181"
                     /pseudo
     CDS             1..796
                     /locus_tag="OO327_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            911..1252
                     /locus_tag="OO327_004182"
                     /pseudo
     CDS             911..1252
                     /locus_tag="OO327_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1235..2072
                     /locus_tag="OO327_004183"
                     /pseudo
     CDS             1235..2072
                     /locus_tag="OO327_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          514 a          504 c          632 g          434 t
ORIGIN      
        1 gggccttgcc cgtagcagtt atttttatca ccgggctgca ctgaaagccg gtgataaata
       61 cgccacgata cgtaagatgc tgacagatat ctttaacagt aattaccagt gttatggcta
      121 tcgtcgcctg catgcgatgc tcaggcatga gggggggcgg ctatcagaaa aggttgtacg
      181 cagacttatg gtggaggaac agcttgtcgt cagtcgtaac cgtcgtcgtc gctacagctc
      241 atattgcgga gaaatcggac cggctccgga taaccttatc gccagagatt ttaaggcgga
      301 gcaacctaat cagaaatagc tgacagatat cacggagttc cagctccctg caggtaaagt
      361 ctggctgtca ccagtggtgg actgcttcga tggaaaagtt gtgagctggt ctctcagtac
      421 acgccctgat gctgaactgg tcaacactat gctggatagc gctgtcgaaa cgttaaatgc
      481 tggcgaacga ccggtgatac acagtgacag aggtgggcat tatcgctggc caggctggct
      541 ggaaagagtg aatgcagcag gtcttattcg ctcaatgtcc cgtaaaggat gttcacctga
      601 taatgctgca tgcgaaggct ttttcggcag actgaaaacg gaaatgtatt atgggcgtaa
      661 atggtcgggc atcacgccag aaaagttcat gcagcaagtg gatgcttaca tcagatggta
      721 taacgagcgg cgtataaaat tatcgctggg tgccgtcagc cccaaaatgt accgccaaca
      781 atgcgggctg gaatgataaa gcagtccagg aaatcgtccg catcccccct tcaccatttg
      841 gattgtccgc tccacccaac atgttgtttc cttaaggttc ctacaccaca aaggaaactc
      901 tcatgctgcg cagtggatgg ctgacgttgc cgacaggcgg gaacttcgcc agttcaggca
      961 gacaccggaa cagcgcttca cgcaggaaca ggagcatctg cagccgctgc tgggtacgga
     1021 cttcgatatc cgtcatgtct cctgggatgg ctatatcgag gttggcggga accgttacag
     1081 tgtgccggaa agcctgtgtg gtcagctggt atcaatacgg atctcgctgg atgatgaact
     1141 gcggatctac agtaatgagc aacaggtagc atcgcaccgt ctctgttcgg ctgcatacgg
     1201 ctggcagacc gtgccggagc atcacgctcc actctggcag caggcttcgg agcgcatggc
     1261 cgaatggctg ggggagatac agaaacgggt aatcactgtc tgtgaccggg aagcggatat
     1321 ctggcattac ctgtattata aagtcagcca cggacagcgt ggtgcgtgct gcacagaatc
     1381 gccggctgga agaggcaccc ggcaagctct tcgaactgcc ggaagtcctg gcaaccgcag
     1441 gaagccacac gctgaatgtg atgcaaaaag gcgggcgggc agcccgtcag gcccggatgt
     1501 tcatcagtta cagcgaagtc agcataaaaa atcccgacaa cagcggccag gcgctcccgc
     1561 tcacgtatgt ctgttgccgg gagcaggcag aggacggtgc ctgctggcat ctgctgacgt
     1621 caggaaaagc ggcgagtgcc gccgatgcac gacgtattgt cagccattac gagcgacgct
     1681 ggctgacaga ggaataccac aaggcgtgga aaagtggtgg tacatggaat cgctgcgaat
     1741 gcagacccgg gataaccttg agcgcatggt ggttatcctg gcgtttatag cggtacgggt
     1801 gctgggcctg cggcagggag gtgtcagtga agaaacacaa aatgacagct gtgagaaaat
     1861 actgacgccg acagagtgga aactactgtg ggttaagctg ggaggaaagc cgcttccggt
     1921 gcaggcaccg accctgaaat gggcctgggg cgatggcatg acagcaaacg cacaggtcgt
     1981 cccggttggg gcgtcatgtg ggatggctgg tcagacttca ggatatggtt gagggttacc
     2041 tggtgatgaa gtctcttgat caggagctct gatcaagaga cagc
//
LOCUS       DAJTSI010000304         2033 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000304 DAJTSI010000000
VERSION     DAJTSI010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.311"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(211..375)
                     /locus_tag="OO327_004184"
     CDS             complement(211..375)
                     /locus_tag="OO327_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020493.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1277..1561
                     /locus_tag="OO327_004185"
     CDS             1277..1561
                     /locus_tag="OO327_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6020494.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1549..>2033
                     /locus_tag="OO327_004186"
     CDS             1549..>2033
                     /locus_tag="OO327_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6020495.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          523 a          427 c          582 g          501 t
ORIGIN      
        1 tggacattat ttttgtggaa ccggaggtaa cagaccagac ggtttaaatg agccggttac
       61 tttaaacggg tgatggacag cacctcaaaa gttcagatgg attatatttg tctgcaatac
      121 cccgggtatt ttcgatttgc gaaatggatg gaacgacttg ctcaggggat agctgatgga
      181 gttactgaag taccgaagga ccactaaaaa tcacaggccg ggaatttcgg cgtttccggc
      241 gtaggtgtgc cacgaatgca tccagtggcg tgatgttgga tgcaatgctg tatgagagat
      301 gaggggcggc gtccagacgc agccttcaaa ccgccttgag ctgctgccgt taagagcggt
      361 ggtggtgagc gtcaaggtta aaaggcgcag aacatgtgtc aggtaggtgt tgcggagatg
      421 aacgaaagtg aactgtcgat gaagtgtcga aaggaacctc taggcgatgt cgaaaatgtg
      481 ctatctacgc agcgcatgac gagtccagca gatatctggt actggctggg cggcatccgg
      541 catataggca gcatgaacgc aacacaggct ctcgtacgga acgtgggaac ctgtcgtctc
      601 gatgctaagg gagcggcgcg gagcagaggc actgcaagcg cctgagtacc gatgcgagac
      661 acaggggcag atggcttcgt agtagtgagg aagtgcctgt aatgggcatg gagcgaaggg
      721 ggtcagtcgt tggggctggt ttatcggtca accggatagc cgggaggagc ccatgagtca
      781 gccaaagccg ttcgaggtct ctaagtatgc ggtttggaaa gcataccagc gggtcaaagc
      841 taatcgaggt gcggcaggtg tcgatgggca gtcagtcgag gcgtttgagg ttaccaatcg
      901 aagctatacc cggtaatggc gcaggttgac ctgcacttgg ccaagtggat tgcccgcaaa
      961 cacaagcgag cgcgaggtag tctggtgcgt gcattcgagt ggttactgcg aattcgtcac
     1021 gactgcccga cgctgtttgc acactggtgc ttagcttacg atacttgaat ggttcaacga
     1081 caagagccgt atgacgcgag agtgtcacgt acggctccgt gggagcgtag gggggcggtg
     1141 cccctgcgcc acccgatatc ccctttgagt gcggttacgt ccacagaggt aaatgccgct
     1201 gacgcggcat ctgtaatctg ttactttgtg tatatcattt gctatatatt gtcaatatgc
     1261 tattgttgta aagcaaatga tatacggagg ctttatgaaa tcaggtgttc agctcaatct
     1321 cagagcaaga gaatcacagc ggatactcat tgatgctgct gcagaaattc ttcataagtc
     1381 gcgtacagat ttcattctgg aaatggcctg taaagctgct gaagatgtta ttctcgaccg
     1441 gcgggtgttt aattttaatg acaggcaata tgaagagttc attgaaatgc ttgatgctcc
     1501 ggttgcagat gatcctgcca tcgaaaaatt gctggcaagg aaacctcaat gggatgtgta
     1561 actgcaccag aacctttatc ctcatttcat caggtcgctg aatttgtcag cagtgaagct
     1621 gtacttgatg actggttaaa gcagaaggag ctaaaaaatc aggcaatcgg agccacccgg
     1681 acttttgttg tatgcagaaa aggtacgcaa caaatagtcg gtttttactc actggccact
     1741 ggtagtgtca atcatacaga agctacaggt aaccttcggc gcaatatgcc agaccccatt
     1801 cctgtaatta tacttgcacg cctggctgta gatgtgtcat ttcgaggaaa agggcttggc
     1861 gcggatttac ttcatgatgc agttcgtcgt tgttacaggg ttgccgaaaa tatcggtgtg
     1921 cgtgcaatta tggttcatgc gcttacggaa aacgcaaaac agttttatat tcatcatggt
     1981 tttaagccat caaaaactca ggtgcaaaca ttgtttctta aactccctca ata
//
LOCUS       DAJTSI010000305         2032 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000305 DAJTSI010000000
VERSION     DAJTSI010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.312"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..362)
                     /locus_tag="OO327_004187"
     CDS             complement(<1..362)
                     /locus_tag="OO327_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6020496.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(429..1643)
                     /locus_tag="OO327_004188"
     CDS             complement(429..1643)
                     /locus_tag="OO327_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HCR6020497.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          633 a          492 c          413 g          494 t
ORIGIN      
        1 agaccgatac gcattaaaat aaagtggcta aaagaattaa ccattcctgt taatgcctgc
       61 attaatgacc agaatattaa gcctgcgcct aaaactattc gtgggccaaa gcggtctagc
      121 aaaataccgg aaggtagttg tgaaaaacca taagacagag aaaatgcaga aagcaaaaca
      181 ccaaactctg tcgccgataa tcctaactcg ccacgaatcg cttcgcctgc cacgctcaat
      241 gacgaacggt cgagaaaatt aacgatcccc gccataaata ataaaaccag ggtcactgtc
      301 tgaatacgac gaatccgttg cggtttatta cccgcaacac tggttgacac atcaacgtcc
      361 atttttagct ccttgagcgg aattaatcga tttgatgact attcagaaaa taattaagcg
      421 aaccgttatt accagttcaa caatgtgcca tcttccagtc gtgcgacggg gagatactcc
      481 gggtcatacg gatatttcgc cgccagtttc tcatcgaaag aaatacccag ccccggttca
      541 tcgcctgggt gcatataacc ttcctcgaag cgccagctgt acgggaaaac ttcaagcatt
      601 tgttcggaat agcccatgta ctcctgaacg ccaaaatttg gtacccatag gtcaaaatgc
      661 agcgccgccg catgacaaac aggcgataaa tctgaagggc catgcgaacc ggtacgtacc
      721 tgataaagcg atgcaaaatc ggcaatccgc cgcatcccgg taatgccgcc tgcatgggtg
      781 atcgtcgtgc gaatatagcc aatcagctgc tcttcgatta actgcttgca gtcccagata
      841 ctgttaaaca cttcaccgac cgcaatcggc gtcacggtat gctggcgaat cagacgaaaa
      901 cattcctggt tctcggcagg cgtcggatct tccatccaga acaggcggta atcctcaatg
      961 ctcttcccaa agcgcgccgc ttcgatgggg gtcaggcgat ggtgcatgtc atgcaacaga
     1021 tgttcgttaa aaccgaactt attacgcacc gcttcgaata acttcggcgt aaaatcgaga
     1081 tacttttctg aagaccacaa ctgctcttct ggccacaggc ctttggttgc cggttcatac
     1141 gccagtcctt tgcccttcgc caggccataa gtggtttgca tacccggtac accgcactgc
     1201 acacgaatcg ccttaaagcc catctgctgg tgctttgcat agtcttcaag gacttcatca
     1261 atcgtacgcc cggtggtgtg gcaataaacc atcacgcctt cacgagaagc accaccaagc
     1321 agttgataca atggcatatt tgccgcttta gctttgatat cccacaacgc catatcaatg
     1381 gcagaaatgg ctgacatcgt gacggggcca cgtcgccagt aagcaccttt ataaaagaat
     1441 tgccagatat cttcgatacg gctggcatcg cggccaatta gttgtggaca aagatgatct
     1501 ttcagataag aagcgaccga caattcgcga ccatttaacg tggcatctcc taatccacaa
     1561 agtccgcttt cagtggttat tttaagcgta acgaaattac gccctgggca agtaacaaaa
     1621 acatctgcag caataatttt cattattgca atctcctgtg ttgtaaatag acgtttcaaa
     1681 cctaatttta cgcctactac catacaagag agaaaaagcg attattcaac cgtgatcaca
     1741 ttaaaaacaa aaatttctac cgaggagaaa aaataccctc aaaaaccacc tgaataatta
     1801 accattattg ttaaaacaac acgttgaata atcacataac aaactccatt aatcattgaa
     1861 aatgagcaca gaaatagaaa ttaattatat tcaattaatt aaaatgtgta ttgcttgatg
     1921 tatttaaaaa tattattttc atggtgagag tggaggtaaa gaagacacaa taataaaaag
     1981 gaatgtgaca aataatggtt ctggttgggc gttaaattta aggtcaccag aa
//
LOCUS       DAJTSI010000306         2030 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000306 DAJTSI010000000
VERSION     DAJTSI010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.313"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..2030
                     /gene="sat"
                     /locus_tag="OO327_004189"
                     /pseudo
     CDS             1..2030
                     /gene="sat"
                     /locus_tag="OO327_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
BASE COUNT          746 a          356 c          398 g          530 t
ORIGIN      
        1 tacggcatga tgatttataa aaatgataaa acatttcgta atcttgaaat attcggagac
       61 agcggctctg gagcatactt atatgataac aaactagaaa aatgggtatt agtcggaaca
      121 acccatggta ttgccagcgt taatggtgac caactgacat ggataacaaa atacaatgat
      181 aaactggtta gtgagttaaa agatacctat agtcataaaa taaatctgaa tggcaataat
      241 gtaaccatta aaaacacaga tataacatta caccaaaaca atgcagatac cactggtact
      301 caagaaaaaa taactaaaga caaagatatt gtgttcacaa atgggggaaa tgttctgttt
      361 aaggataatt tggattttgg ttgcggtggt attatctttg acgaaggcca tgaatataac
      421 ataaacggtc agagatttac atttaaagga gcaggaattg atatcggaaa agaaagcatt
      481 gtaaactgga atgcattgta ttccagtgat gatgttttac acaaaatagg ccccggtact
      541 ctgaatgttc aaaaaaaaca gggggcaaat ataaagatag gtgaaggaaa tgttattctt
      601 aatgaagaag gaacatttaa caatatatac cttgcaagcg gaaatggtaa ggtaatacta
      661 aataaagata attcccttgg caatgatcaa tatgcgggga tattttttac taaacgtggt
      721 ggcacgctag atttaaatgg acacaatcag acttttacta gaattgccgc cactgacgat
      781 ggaacaacaa taactaactc agatacaaag aaagaagccg ttctggcaat caataacgaa
      841 gactcctaca tatatcatgg gaacataaat ggcaatataa aactaacgca caatattaat
      901 tctcaggata agaaaactaa tgcaaaatta attctggatg gtagtgtcaa cacaaaaaat
      961 gatgttgaag tcagtaatgc cagtcttacc atgtaaggcc atgcaacaga gcatgcaata
     1021 ttcagaagca cagcgagtca ttgctccctg gtatttcttt gtggaacgga ctgggtcacc
     1081 gttttgaaag aaacagagag ttcatataat aaaaagttca attctgatta caaaagtaat
     1141 aatcagcaga cctcatttga tcagcctgac tggaaaaccg gggtgtttaa atttgataca
     1201 ttacacctga acaatgctga cttttcaata tcacgcaatg ccaatgttga aggaaatata
     1261 tcagcaaata aatcagctat cacaatcggc gataaaaatg cttacattga taatcttgca
     1321 gggaaaaata ttactaataa tggttttgac ttcaaacaaa ctatcagtac taatctatcc
     1381 ataggaaaaa ctaaatttac aggtggcatc actgcacata acagccaaat agccataggt
     1441 gatcaagctg tagttacact taatggtgca acctttctga ataatactcc tataagtata
     1501 gataaaggag caaaagttat agcacaaaat tccatgttca caacaaaagg tattgatatc
     1561 tccggtgaac tgactatgat gggaatccct gaacagaata gtaaaactgt aacgccgggt
     1621 ctccactacg ctgctgatgg attcaggctg agtggtggaa atgcaaattt cattgccaga
     1681 aatatggcat ctgtcaccgg aaatatttat gctgatgatg cagcaaccat tactctggga
     1741 cagcctgaaa ctgaaacacc gactatatcg tctgcttatc aggcatgggc agagactctt
     1801 ttgtatggct ttgataccgc ctatcgaggc gcaataacag cccccaaagc tacagttagc
     1861 atgaataatg cgatctggca tctaaatagc cagtcatcaa ttaatcgtct agaaacaaaa
     1921 gacagtatgg tgcgttttac tggtgacaat gggaagttta caacccttac agtggacaac
     1981 cttactatag atgacagtgc atttgtgctg cgtgcaaatc tggcccaagc
//
LOCUS       DAJTSI010000307         2028 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000307 DAJTSI010000000
VERSION     DAJTSI010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.314"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1823)
                     /locus_tag="OO327_004190"
     CDS             complement(<1..1823)
                     /locus_tag="OO327_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR6020498.1"
                     /translation="MRPSELSDLLWAQVDRVAPHLLPNGKIEGHEWVAGNVNGDKGNS
                     LKVNLIGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDARR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEVVKRYEVVSGKVWNGERELDALVL
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRVVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIVSR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKQQYLFNPPWESLADKFQFREAELTLVNGVHGHGKACPLNEPILLADGTWTTHGNV
                     KIGDQVASVDGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEK
                     RRVIDTFGLKRLSETKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKF
                     SNVEPYMIERMKAELPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFI
                     PRVFFSANKSTRIGMLCGLLETDGYVEKDGTLVFSSASEELR"
     gene            complement(1931..>2028)
                     /locus_tag="OO327_004191"
     CDS             complement(1931..>2028)
                     /locus_tag="OO327_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6020499.1"
                     /translation="WNDEKPETIQPQSRPSSAITVSKTGYVFFDR"
BASE COUNT          485 a          579 c          429 g          535 t
ORIGIN      
        1 cgcagttctt cacttgctga ggaaaaaaca agcgttccat ccttctcgac atacccatcc
       61 gtttcaagca gaccacacag catgcctata cgcgttgatt tatttgcgga aaaaaacaca
      121 cgagggatga atttgttttt tgctgtgcac cccattagtc cataacctcg cagggtctcc
      181 atgagtggat ttacctgacc acgcgccgtt gatatcagcc agtccttacc atctccagag
      241 aagttgtaat caggcagttc agccttcata cgctcgatca tgtatggctc gacgtttgaa
      301 aacttcacgc tcccattgct aagactacca tccccgagaa gggagccgat aacccatgct
      361 aatggctctg agtggtcgcc aaagtcacca gttatttcag gaatcctaac gccatttttg
      421 tgcctcttcg tttcactcaa ccgcttcaac ccgaaggtat ctatcacgcg gcgtttctcg
      481 cctttcgtga atccacgact agtgacctcc cataggtgat cgcctgcgca atcaacataa
      541 cgaccatctt caaatgtgac tcggtaaaca tctctaacac cctgcgggaa tatcccagtg
      601 acagttgacg gattgccgtc tactgacgcc acctgatcgc caatttttac attcccgtga
      661 gtagtccatg tcccatcagc taaaagaata ggctcattca gtgggcatgc ctttccgtga
      721 ccgtgcacac cattgaccag cgtcaactct gcctcacgga actggaattt atctgccaga
      781 gattcccacg gtggattaaa cagatactgc tgcttgccgt agaaagcgtt gatagtgtcc
      841 tggtaaaact ctcgcgcact gtagagttct tcaggatcga agtaggatgc cgtgccgatg
      901 tactgccaga tttcatcctc ggtaacaccg ttcatcaggc attcgttgat gtctttgtac
      961 ggcagagtaa caagacggca acgatgttca ccgagtcggc ttacgatttc ccttgcggct
     1021 tcacgaccaa catcatcaac gtccatcgag atgaatattt cctcaaacct gtcgaggttg
     1081 tgatactcaa actcaatcca ctgttgctta gcgcctttcc cgccaccaaa cggcacggat
     1141 aacgccgaga tgccgtattg cgcatagctc atacaatcaa tttcgccttc gcaaagcaca
     1201 accaccctca cgccagcgtc cagagcctgc catccgaaca ggcaaggttc gcaatcacct
     1261 tctgccataa tgactttctt cccgtccggg cgctcagtgc tgattcgctt gacctgcaac
     1321 aactcaccat cgcgtttgta cggaagcacc agagcatcca gttctcgttc tccattccac
     1381 accttgccgc tgacaacctc gtagcgcttt acgacttctg gcgatatgcc acgcgattgc
     1441 aggtactcaa gatgggattc tgttctggta acgtagcggg cgattttctt acgatcaggt
     1501 ctggagaatt tcttctcacg tctggcatcg aaatggtgat cgtcatcctt gataccgaga
     1561 aatgctttcg cttcctgcat agcctgatgc aggttaattc cacgacatgc catccacaaa
     1621 tcaagcatgt caccgccgtc tccctcagcg aaatcagccc attttttctt gccgataagg
     1681 ttgaccttaa ggctgtttcc cttgtcaccg ttgacgttac cggcaaccca ctcatgcccc
     1741 tctatcttgc cgtttggcaa caggtgcgga gccaccctgt caacctgcgc ccaaagcagg
     1801 tcgctaagtt cacttggcct catgattccc tcagattgag atttttaaac cagaaatcga
     1861 caaacgaaat acttaaccag ccgtggttat aaccagcgac cagtagcgat ttgatttttg
     1921 atttcatggt tcacctgtcg aaaaacacat agccagtttt cgatacggtg attgcggatg
     1981 atggtctgga ttgtggttga atggtttctg gcttctcgtc gttccagc
//
LOCUS       DAJTSI010000308         1983 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000308 DAJTSI010000000
VERSION     DAJTSI010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.315"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..774)
                     /gene="gshA"
                     /locus_tag="OO327_004192"
     CDS             complement(<1..774)
                     /gene="gshA"
                     /locus_tag="OO327_004192"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR6020500.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYE"
     gene            complement(847..1275)
                     /locus_tag="OO327_004193"
     CDS             complement(847..1275)
                     /locus_tag="OO327_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6020501.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(1272..1838)
                     /gene="yqaB"
                     /locus_tag="OO327_004194"
     CDS             complement(1272..1838)
                     /gene="yqaB"
                     /locus_tag="OO327_004194"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR6020502.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT          546 a          518 c          457 g          462 t
ORIGIN      
        1 ctcgtaaaga tcgttgaagg taataccaag attgctttgc gatttattgg tatagccgag
       61 atcgctcaaa cgaagagagg tcgcatacgg caggtaatac ataccgcact cggttttctc
      121 aaacggcagc gacgttggtt ttccttgcag gaaagaagaa caaatcgccg gagatgcacc
      181 aaacagataa ggaataaccc aaccgaaacg atagtaattg cggataacgc ggaaatagcc
      241 cgcagaaatt ttctctttgg catcagcgcc cgagatatca ccgcacttcg cttgccagaa
      301 tgccattggc aaagagaaat tgtagtgcac gccggaaatg gtttgcatca gcgcgccgta
      361 gcgatttttc agcccttcac gatacagcgt tttaaagcgt ccggtgttag aagtgccgta
      421 ctgtgccagt tcgatgtcct gaccttctgc gatgtagcat ggcatactta acggccacat
      481 ccgctcatcg cccatattgc gcgccgtata acgatgcaga tcgcgcataa aggtcagcat
      541 atgttcaata tcaccatcca ctggtgtaat gaattccagc aatgcttccg caaaatcggt
      601 agtaatccat ttgtgcgtca gtgcggaacc taatgcttca ggatgacctg ttgttgccag
      661 tgtgccatca gcattaacac gcaaagtttc gcgctccagc ccacgctgta tcccctttaa
      721 cgcctgagga tgtttttcca gccaggccag cgcctgtgat acgtccggga tcaaattgac
      781 ctcccgcctg tcaaaatcgt tttaattagc ataactgtaa tggtgaccat atgtgcaggc
      841 ctacaattag tgccaccaca tcatgccctg aacggtcgct gctgcaactg caacatagcg
      901 taacgcttta ccaaggcaca aaaaaaagat tactggcccc caggagatgc gcatccatcc
      961 cgctaacaga cacagtaaat cgccaaccac gggcatccag cttaataata gcgtgactgc
     1021 accatagcgt ttcagccagc cggtagcttt ctcttgccag cgcgatgttt tacgcaatgg
     1081 aaagaaacgc ccaaggataa cgttagttaa ccctccaagg ctattaccca ttgttgctgt
     1141 taaaactaaa acccagggat gactgatccc ggaaagcaac attgccacca gcacgacttc
     1201 ggagttgccg ggtaatagtg tagcgctgag aaaactactg gcaaacaacg agaaaagcga
     1261 taacgcttca ctcacagcaa gcgaacatcc acagcgtcca tgcctgctgc acgggccgcc
     1321 tgaataccga aatcggcatc ttcaaagacc acacactgcg tcggttgcac gcccatacgc
     1381 tgcgcgcaca acaaaaatgt gtctggcgcg ggtttatggt gtttgacgtg atcagcagcg
     1441 acgacggcgt caaaataacg gcgtaatccc aggtgcgcca gcaatgcctc agcgatggcg
     1501 ctttcactcc ccgttcctac agccattggg cgacgaccat gccaactttt caccacttca
     1561 acaagaggaa gcggctcgac actatccagc agcatacttc ttactgcttc tgttttttca
     1621 cgcgctaacg catgcgggtc gagatcggcc tgattcagct caataattgc ctgagcaata
     1681 cgccaggtgg gcgatccatt aagcgcaatc atcgcctgaa tatcgtactg aagaccgtag
     1741 tgccctaata cttcgcgcca cgctttacgg tgcgtaggcg ccgtatccag gattgtgcca
     1801 tccatatcaa aaattaaacc tgcataacgc tcgtacatgg tcttctcgca agtcgaaaaa
     1861 tatgacgcta ctttagcgta attgcttgat tttgtcgctg atggcgaagg agaagaaaat
     1921 tcaggaaatg gataaagtag ttatttagaa atgagatatt tttgaaggaa attttttgga
     1981 gaa
//
LOCUS       DAJTSI010000309         1951 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000309 DAJTSI010000000
VERSION     DAJTSI010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.316"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            39..1436
                     /gene="ccp"
                     /locus_tag="OO327_004195"
     CDS             39..1436
                     /gene="ccp"
                     /locus_tag="OO327_004195"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR6020503.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1647..>1951
                     /locus_tag="OO327_004196"
     CDS             1647..>1951
                     /locus_tag="OO327_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6020504.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCV"
BASE COUNT          539 a          403 c          493 g          516 t
ORIGIN      
        1 tctgggtcac aataactcac caccgtgagg gaagaataat gaaaatggtc tcacgtatta
       61 ccgcgatcgg cctggctggc gtcgcgattt gctatttagg gttatctggt tatgtgtggt
      121 accacgataa taaacgcagt aaacaggccg atgttcaggc atctgctgtc agtgaaaata
      181 ataaggtttt aggctttctc cgcgaaaaag gatgcgacta ttgccacacg ccttcggcag
      241 aattacccgc ctattattat attcctggcg cgaaacagtt gatggattac gacattaagc
      301 ttggatataa atcttttaac ctcgaggccg tgcgtgcggc actgctggct gataaacccg
      361 tttcgcaaag cgatctgaat aagattgaat gggtgatgca gtatgaaact atgccaccaa
      421 cgcgttatac cgcgctacac tgggcgggta aggtgagtga tgaagagcgg gcggaaatac
      481 tggcctggat tgcaaaacag cgcgcggaat attacgccag caatgatact gctccggagc
      541 atcgcaatga accggtgcag cccatcccgc aaaaactgcc taccgatgcg caaaaagtgg
      601 cgttgggctt tgcgctgtat cacgatcccc gtttatcggc tgatagcacc atttcatgcg
      661 ctcattgcca tacgttgaat gcggggggcg tcgatggcag aaaaacatcg attggtgttg
      721 gtggcgcagt tgggccgatt aatgcgccga cggtatttaa ctcagtattt aacgttgagc
      781 agttctggga tggtcgtgcg gcaacattgc aggatcaggc aggtggaccg ccgttgaacc
      841 cgattgaaat ggcgtcgaaa tcctgggacg aaattattgc taagctggaa aaagatccgc
      901 agcttaaagc gcagttcctc gaagtttatc cgcaaggttt cagtggcgaa aatattactg
      961 atgccattgc tgaatttgag aaaacattaa ttacgccgga ttccccattt gataaatggt
     1021 tgcgtggaga tgaaaatgcg ctgacggcgc aacagaaaaa aggctatcaa ttatttaaag
     1081 ataataaatg tgcaacttgt catggtggta ttattctcgg cggacgttcc tttgaaccgt
     1141 tggggctgaa aaaagacttt aactttgggg aaattacggc ggcggatatt ggtcgtatga
     1201 atgtgactaa agaagagcgt gataaattgc gtcagaaagt acccggttta cgtaacgttg
     1261 ctttaacggc accgtacttc catcgcggtg acgtgccgac gctggacggg gcggtgaaac
     1321 tgatgctgcg ctatcaggta ggcaaagagc tgccgcagga ggatgtggat gatatcgtag
     1381 ctttcctgca cagtctgaac ggggtgtaca cgccgtatat gcaggataaa caataattaa
     1441 tttgatcgcc cgaacaggaa tgtttgggcg atttttatta cgataataaa gtctgttttt
     1501 aatattatca tgttaaatgt ttatattata aaaagtcgtt tttctgctta ggattttgtt
     1561 atttaaatta agcctgtaat gccttgcttc cattgcggat aaatcctact tttttattgc
     1621 cttcaaataa atttaaggag ttcgaaatgg accagaagct gttaacggat ttccgctcag
     1681 aactactcga ttcacgtttt ggcgcaaagg ccatttctac tatcgcggag tcaaaacgat
     1741 ttccgctgca cgaaatgcgc gatgatgtcg cattccagat tatcaatgat gaattatatc
     1801 ttgatggcaa cgctcgtcag aacctagcca ctttctgcca gacctgggac gacgaaaacg
     1861 tccataaatt gatggatttg tcgatcaata aaaactggat cgacaaagaa gaatatccgc
     1921 aatccgcagc catcgacctg cgttgcgtaa a
//
LOCUS       DAJTSI010000310         1913 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000310 DAJTSI010000000
VERSION     DAJTSI010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.317"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(53..1702)
                     /locus_tag="OO327_004197"
     CDS             complement(53..1702)
                     /locus_tag="OO327_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR6020505.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          452 a          506 c          491 g          464 t
ORIGIN      
        1 gaagaggata attttacagc cagcaggaag ttagcggctg gctgtaaaaa tattatggtt
       61 cgccgtacaa accaattaaa cggcgtacca caccgttagt ccagccaaac ccatcctgca
      121 acggatactc gccaccgccg ccttcgcggg gaacaccatc ggcaatatgg tatttctcga
      181 tcattttgtg ctgttccaga tagaactgat tcaccgtttt cagccagctg cgcgcgattt
      241 catcacccag aaggtcatcg ccatacattt taaatccctg aattgccatc cattgtaacg
      301 gtgcccagcc gttgggttta tcccactgtt caccggtttc gtactcgctt gccaaaatcc
      361 cgccaggtgt cagtaaccgg ctgcgtacgg cgtttgccag acgatcggcc tgttcatggt
      421 tcgccatgcc gacatagagc ggcacaatgg cggcagcgga aaacagcgcc agttgttcgc
      481 gtcgccagtc gtaatcgcgg tagatgccgt tttcatcatc ccagaggtaa cggtttaccg
      541 catcgcgacg ggcactggcc ttctggcgga acagcgcttc tgtctctttc tcgcctttca
      601 gcgccgagat gttggcgatg gcgctctcca gtttaaacag gaaggcattc agatcgatgg
      661 ggatgaactg ggtggtacga atgctcgcca gacgaccagt atcacgcagc caacgggaag
      721 agtaatccca accggaggcc gctcccgcgc gtaaatcgcg gtacacctcg ttgggcgggc
      781 gaccagaatg tttcgcggtt tcaacgtcct caagccagga ttcgtcacgc ggcgtgtcgc
      841 gatcgtccca gtaacggttg agcagcgatc cgtccggcat ccgcacaaca tggcgatagg
      901 cctgattagg gatcaacgat tctgcaccgt ccatccagaa ggcatattcc attttaaggt
      961 ggtcgagata gcggcgcgca ccgcgtacac catcttcttc aaacaactcc accatcagcg
     1021 caaaaaccgg tggttgcgag cggctcaaat aataggtgcg gttgccgttg gggatgtgac
     1081 cgtagttttc gatcatccag gcgaagttat cggccatgca tttcagcaaa tcttcccgac
     1141 cactttccgc cagtcccagc atggtgaaat aggaatccca atagtaggtt tcgctaaaac
     1201 ggccgcccgg gacaatatat gactgcggta gcgccagcag agaagaccac ggaatgtgat
     1261 cctgtggttc gcgggttagc accggccaca gctggtcgat atgctctttc agggaatttt
     1321 gcgggtccga tacatactcg ctggagtaga cctccggcag ccagaagtga ttttcaacaa
     1381 acttgcgcaa gtcaaaatca cgatgacggc gcactttacg gtagcggatt aaaatatcca
     1441 gcgggtccat tttgggtgcg cagtcgggaa aggttttact gtcggggaaa attttcgccg
     1501 actgaacatg ctcgaacagt tcgagatagc gatcggcagg cgtcagcgca tcagaggcgg
     1561 gcagcccttc aatcatctcg ggttccggtt ctgcctcgat catctcatcc agttttaatt
     1621 catacgggtc cagttcatag cagagatcga cctcgatcat cagttcgtct ggattagggt
     1681 tttgaatttt ctgattgagc ataaggatcc aacctccgaa attcgtcgct aattatcggg
     1741 cttttcttta agggtagtca ttcgctccgc ctggtgggcg acaaagcgtg cggtggatca
     1801 cgtttattaa cttgtcaaaa aatataacgc actaatatca atggaattta taaaaatacg
     1861 gatttggtgt tgtttttcat tttcagaaca ttcaatcctg ctctcgccgc aat
//
LOCUS       DAJTSI010000311         1913 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000311 DAJTSI010000000
VERSION     DAJTSI010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.318"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..836)
                     /locus_tag="OO327_004198"
                     /pseudo
     CDS             complement(1..836)
                     /locus_tag="OO327_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
BASE COUNT          512 a          451 c          443 g          507 t
ORIGIN      
        1 gctgctctac ggtcatattt ttaattgcgc tccggtttac aagagcccac ccctgttttt
       61 ccagataaaa ctggaaagta tccagggtac agaccagtgc accgtcagga acagtttcgg
      121 tgaatctgat attgccgtgt tcgtcgaagt gcacaaccag agtgcgaccg ttacccggaa
      181 tcatcttgtc agcggacggg gtattattct ggtgcagttc tgcctccatg cggtcgaaca
      241 tcagcgatgt aggcctcttt gaatgcagcg gcttttttcc cagtgaagcc catcaccagg
      301 aaaacgaagc cgtttttggt gatttggtac attgggcgtt tttcgccttt ggtgtcggtg
      361 taggtgacgt cctcaaaatt gagggcgtta aattcaggtg agcattcagt atttgcgatg
      421 gcacgtaaca cattgtcgtg tcgtttgtgg aagaactctg caaccgcaac agacgtagtg
      481 acagcgcgac cgttttcgat ggttacgtca gggtgagaaa gggcagggat agtagccatg
      541 atggcatcct cgagtgataa gttaattaac tcaccaccga ggttttccac gaccataagg
      601 gtggtgagac gtacaggggt ggaaataccg gtcactcgag aacccggcca gtcttgcgac
      661 tgccctgcac atcccaccat aatttgaatg gagctgtgca ttacgcataa aaaaaccgct
      721 tcagcgcggc tatgcgctcg agtaacgttc gggtttccac gcccggcccc cgttttatga
      781 ggtgcagatg cactataatt ccacccgttc tggttttcaa tagctacatt caacattttc
      841 tcttaccttt catcaccgaa gtgaactttg ttgatgcggt gcctggtgcc tccaggtgac
      901 gttaaccagt taacaattaa cgccggatac agggaaaccc ataaccaccc gatacgtggg
      961 aactaatcgc tttttaactg ttccgcgtgc gctcagccgc attcaccgca tcacaaaatt
     1021 cactttaaaa agggcggaca tcagccagca atgaaactga tgccgccaaa ggtaccaatc
     1081 aacatggagt gttgtggcgt ggttgtcact taagcgtatg gtcaacctga caacccggtg
     1141 tcctcaacgg ggaaggaata actccgccat acttaccgcc gcgtcatttc gcgttgtgtg
     1201 cctgctttta actacgtcag gcgaggtggt tcctgttatt ccccaacaac aagaaatttg
     1261 tataatctga ataccccaac aataagaaga gtgagcaagt gatgtattag cgatgtcagc
     1321 tctcaaagag actgcatcaa tgttaaaatt attcaatgaa gcaaaaactc aggctgaggt
     1381 tgatcgcgct gtgtctgaaa taaactctaa gttgtcaagc attcagcttg aatatttgtc
     1441 atctcattga tgagctgaca atctctaaaa gcaatgaggc tgcgctcaaa gcaaaactca
     1501 cggagataga aaagtttaag ttccaaagtg aagactgcga acctcgctaa ttgattactg
     1561 gaacgattgt gtatgccaga cagtgcaata ctagctctgg caagattacc atgtattttt
     1621 gtccagaatg ttttgcgaag agagcaatat ctatattgca acctttccct atcgaggaag
     1681 gcgataaata tacgaaaact caatgcccct cctgtaaaaa tgagtatctc atggactaca
     1741 atttcaataa tgatttttga gtcacggtat tcagattttt aaagaacatg ccggatactc
     1801 acccgtgtcc ggcgcacgca ctccacctca cccgtggaga actccttaat taccaacctt
     1861 ggcttcgttg gttagccatt aacgctggta tgtaatcatt ctggcaatgc tta
//
LOCUS       DAJTSI010000312         1912 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000312 DAJTSI010000000
VERSION     DAJTSI010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.319"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            193..462
                     /locus_tag="OO327_004199"
                     /pseudo
     CDS             193..462
                     /locus_tag="OO327_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            459..685
                     /locus_tag="OO327_004200"
                     /pseudo
     CDS             459..685
                     /locus_tag="OO327_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            714..1304
                     /gene="ospG"
                     /locus_tag="OO327_004201"
     CDS             714..1304
                     /gene="ospG"
                     /locus_tag="OO327_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HCR6020506.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     VYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
     gene            1643..1912
                     /locus_tag="OO327_004202"
                     /pseudo
     CDS             1643..1912
                     /locus_tag="OO327_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          540 a          412 c          454 g          506 t
ORIGIN      
        1 ctgtcagcgc cagtgatagg acaggtgaaa cctgtcaata cgtaccggct tacgccgggc
       61 ttcggcggtt ttactcctct gccatatgca acaacggagt gccgccttac atgccgctgg
      121 cgcggcatat cctggtgacg atatctgaac agttatatac tgtgtatata cgtggtaatg
      181 acgaggtggc aaatggcaca ggttaatatg agtgtaagaa tcgacgcaga actgaaggat
      241 gcttttatgg ccgctgcaaa aagcatggac cgtaatggct ctcagttaat ccgggatttt
      301 atgcgccaga ccgttgaacg gcagcacaat tcttggttcc gtgactaggt tgcggcaggc
      361 cgtcagcaac tcgaatgcgg tgatgtgctt ccccatgaca tggtcgaatc ctctgctgct
      421 gcatggcggg atgaaatgag caggaagatt gctgataaat gatggagatg ttctggacca
      481 tgctggccat ccaggacagg aagcgcattc gtagtacatt gcggagcaga acctgatggc
      541 cgccattgaa ccggacgaac ggattggtta ttcagccagc agcctggcag ggcagccgta
      601 taaaggccgc aacggtcgtg tggaaggaac atctggacca cacaaggtag cgtgtaacgt
      661 tattttgtgt gaaaatctct tgtgattttt aaatatcgta acaaaggtat gacatgaaaa
      721 taacatctac cattattcaa acaccttttc catttgagaa taataattct catgctggca
      781 tagtaacgga gcccattctc ggtaagttaa taggtcaggg gtcgacagca gaaatctttg
      841 aagatgtgaa tgattcatct gctttgtata aaaagtatga tcttattggc aaccagtaca
      901 atgagattct ggaaatggct tggcaagaat ctgagctttt taatgctttt tatggcgatg
      961 aagcatccgt tgttatacag tatggcggag atgtgtacct ccgaatgctg cgcgtgcctg
     1021 ggactcccct tagtgacatt gatacagctg atatccctga taatatagag agcctttatc
     1081 tacagttgat atgtaaattg aatgagttga gtataatcca ttacgatctt aatacaggta
     1141 atatgctgta tgataaagaa agtgaaagtt tattcccaat agattttcgc aatgtttatg
     1201 ctgaatatta cgctgcaacc aaaaaagata aagagattat cgaccgacga ttacaaatgc
     1261 gtacaaatga tttttattcg ttattaaaca ggaaatattt atagacgtat ttgttgatgc
     1321 tataagccag aggctcttct atgagaatat ggggaccatt ccctcgcgat attaacgtaa
     1381 tatcttaatt gtatgcaccg ccagaatgcg ttcagtgcga tcacggcagc agacaggtaa
     1441 aaatagcaac aaaccacccg aaaaaccgcc gcgatcgcgt atgataaatt ttaaccacat
     1501 gcatacctgt gcaacaaggc gaatcacgca cggatttcca gccagagaca cctgaaacgt
     1561 aaccggctca tttaaaccgt ctggtctgtt acctccggtt ccacaaaaat aatgtccatc
     1621 attttgaagt ggacactatc gtatggaata ccggacctga atcactgaag ctttacgcct
     1681 tcatttcgaa gaacatttac cacgggttgt ggccggatgt cgcctgggcg taccaaaatc
     1741 aactgtctgt ggtatgttcg tgcgcttccg caacgctggc ctttcatggc ctctgcccgc
     1801 aggtatgtcg gagcaggaac ttgatgccct tctttacgga agtgcctcca cagtacctgt
     1861 cgtacttact gaaagcaccg ttatgcccaa actcccggtg gtaaaaaaac gt
//
LOCUS       DAJTSI010000313         1909 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000313 DAJTSI010000000
VERSION     DAJTSI010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.320"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(120..1574)
                     /gene="ospC2"
                     /locus_tag="OO327_004203"
     CDS             complement(120..1574)
                     /gene="ospC2"
                     /locus_tag="OO327_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR6020507.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDIAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFISNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          608 a          326 c          297 g          678 t
ORIGIN      
        1 gggcgttcac cacatgcaat gtgttgttgc aggggaacgg tctgcaccat cccctatatc
       61 gatcaatata acaaaaatga caacgtcaga ttcagggcac aacctaaata tattttaggt
      121 cacctcccaa atcgtttgcc tgataccgct ccatttttta ataaaaaatc aattgttttt
      181 gaatctttgc ttttcattgc attgtcaagc atagtgtccc cactatttgc tttttgaaat
      241 cttttgttta catcaaccaa tccattacta ataaaatatt ctagcacttt atagttggca
      301 ctatcttcac taagtaaata ctcaacatca tacagctcac aatacatatt gttgttcatt
      361 ttctccatct ctgcgatatc ttgttttgta aaattaaatt tagatagtaa ataaagtgcc
      421 atatcagatt ttgcattgcc tagagaataa gcaagagctt gatgagccat ttttttatca
      481 gtcaccttat tgttaatttc ttcataattg gatgctttaa tggcatcctc caaagatata
      541 tctctgagtc ttatcttttt gaagttatct gtgcttagca ttcttggtat atgatactca
      601 agctgaaaca caaagtttat aattctatcc agactaacag gatcaatgtt tttattataa
      661 caaaactctc ggaatctttg atctttactc tttcttataa aatcaataag atgcaaccca
      721 attccaagcc tcatatcttt agtattaaat attggcacat cagtttttcc ctgattacca
      781 tgcacatacc tgctaacttc atccacaacc tctttaaagt tatctaagaa aaatgattgg
      841 tgctcatggt aaaaaacagg tggaatagca ttatcaaagt ggtcggttaa tgtcatatat
      901 ccatatggag agtcatgatc aatgatatac gcattagcac caaaatccac agtatgatgt
      961 gttttattta gtgggagtgt ttcttgatga ttggaaattt caaccccaaa aaaaacaaaa
     1021 tcgtggttag ctatttcttc aatatcctca gagaatgtgt ttttatatgt ttgtatattc
     1081 tcagacttaa ttttatcaaa ggacttaata ttaagagtat tatttgacag gttagcgtta
     1141 ctttgatgtc tgaagttaaa atcagaatta ataataatag agagcaagtg acgttcatcc
     1201 gaagaaagag gatgagtttt ttgtattatt tcagaaagat agtgtctgga caccttatcg
     1261 aggtgctcaa gtgaccgaaa tgatgaccgt gctccagatg gcgcttcaat agaaagattt
     1321 aatgatttga aactggtccc ttgagagaac attttactat aactttgcgc tgctatagac
     1381 ttccttaaaa agcttttcac agtatcagca cagtgatcta aatgtttttc acttatgcca
     1441 gaagaggatg agtttgttac gcatgatatg tttttttgta atgcttttgt atctttgttc
     1501 ggatttattt ttccatcaac aagatcaata ttgttctgaa cattaatatg atttactgct
     1561 tcaggtattt tcataaaaca tcccccatta tgtgcgtttt tatctgcagt tatttagaat
     1621 aatattatct aacttaatat tttcttgtat gtattataac gaacatattt taatatgttg
     1681 tacgattttc actctgaaat cccctgagtt taatcacact tcctttactg aaaggaatta
     1741 ttgcaacagt attatttgtt tttggatata tatcttgcgc cttgttatgt agttaatgct
     1801 cagaatatga tagataaaat agagacgcaa aacagggcat gctcataaca tgatgataac
     1861 ataaaaaatt agtaacaata aatgaaagaa tcatgggcgc tcaacaaaa
//
LOCUS       DAJTSI010000314         1860 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000314 DAJTSI010000000
VERSION     DAJTSI010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.322"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..344)
                     /locus_tag="OO327_004204"
                     /pseudo
     CDS             complement(1..344)
                     /locus_tag="OO327_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(344..1264)
                     /locus_tag="OO327_004205"
     CDS             complement(344..1264)
                     /locus_tag="OO327_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6020508.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1402..>1860
                     /gene="pgrR"
                     /locus_tag="OO327_004206"
     CDS             1402..>1860
                     /gene="pgrR"
                     /locus_tag="OO327_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR6020509.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVLRCTGNSG"
BASE COUNT          465 a          451 c          433 g          511 t
ORIGIN      
        1 cacacaccca gcacgccaat gcgttcagca tcaacatatg gcagagttac tgcataatcg
       61 ataacacggc taatatcctc tacgcgctgt gtcggatctt ctatccaacg aggctaccca
      121 ccagattctc cctggaaact ggcgtcataa gcgagaacga catagccttt ttcagccaat
      181 gccttgccat agatattgcc agacgtctgc tccttacaac tgccaaaagg atggacgctg
      241 ataatcgccg gatactgttt tgactcatca aaatttggtg gaaactgaat attggcagca
      301 atatgccagg ccatatcact attgcggatt tgaatatgtt tcattacaga gtttcctcaa
      361 agaacggagc taatactgaa attgcttcag ccacataggc tttgccgtca taaagatcca
      421 tatggtttgc gccttcaaca atgtgatagc gtttatcctg gcttgatgcc cgatcgtaca
      481 ggtcgtcact catccatttg ctccctgcct ggctgcccgc cacaatctgc atcggctgag
      541 tcaggtacac ttccgccata tggtaagcat cataggtaat aatctggtta aggctgcgca
      601 aagtagcgta acccggtgct gttggatact gcgcacgagg tgtgtggtaa tactcccagg
      661 cctgacgcag ttcttcgttc ggcgcatcgg cctccttcat tggagccagt ggcataatgg
      721 cgtattcttc gctgctaata tcgctggttc gggcgtttga gccagcttca atgtgtggca
      781 gagcatcaat agatttcaca ttgttttccc aaccattgcg gaacatcgaa ccaatattga
      841 ccgcactaac ggtaccgatg gccttgatgc ggcgatcctg aattgcagca ttggctgtat
      901 atcctgcacc ggcacaaatt cccatcgcac caattcgggt attgtcgaca taagaaagcg
      961 ttgtcaggta atcaatcacg gcactgacgt cttcagtacg aatgtatggg ttttctaact
     1021 gacgcggctc gccgccactt tcaccttgat aagatgcgtc ataagcaata gtgacaaatc
     1081 ccttttccgc cagcttttcg gcataggttc cggccgtttg ctctttaacg cccccacctg
     1141 ggtgagatac cacaattgcc ggatactgac gggtttcatc aaattttgga gggaaataga
     1201 tcactgcaga caaagagatg gttggattat tgctgttagt gaagctgact ttattattca
     1261 tcattcagtt cctcatggag ttgtcggttc gttttgacgg ttggtgatat cactatagat
     1321 attgatcatt aagttgatta gaccctaaat catgattaga cctatcattt aaatgattaa
     1381 tagattgtat ggagatactg aatgaagcgt gaagaaatcg ctgatctgat ggcgtttgtc
     1441 gtcgttgcag aggagcgtag cttcactcgc gcagcagccc gcctgagcat ggtgcagtca
     1501 gctttaagcc agatagtgcg ccgtatagaa gaacgattgg gattgcggct tctgacgcga
     1561 accacgcgca gcgttgtacc cactgaagcg ggcgagcatc ttttgtctgt tcttggccct
     1621 atattgcatg acatagattc tgccctgaca tccctgagcg atctgcagaa ccgcccatcc
     1681 gggacaatac gtattactac tgtagaacat gcagcaaaaa cgatattgtt accagccatg
     1741 cgcacattcc tgaaatcgca tcctgaaatt gatattcagc ttaccattga ttatggtttg
     1801 accgatgtcg tttctgaacg ttttgatgca ggcgtcctga ggtgtactgg caatagcgga
//
LOCUS       DAJTSI010000315         1831 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000315 DAJTSI010000000
VERSION     DAJTSI010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.323"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..179)
                     /locus_tag="OO327_004207"
     CDS             complement(<1..179)
                     /locus_tag="OO327_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR6020510.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGDNFQMYGVIV
                     MTTQISVETLSPITH"
     gene            complement(320..874)
                     /locus_tag="OO327_004208"
     CDS             complement(320..874)
                     /locus_tag="OO327_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR6020511.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(871..1161)
                     /locus_tag="OO327_004209"
     CDS             complement(871..1161)
                     /locus_tag="OO327_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR6020512.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1161..1760)
                     /locus_tag="OO327_004210"
     CDS             complement(1161..1760)
                     /locus_tag="OO327_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR6020513.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          445 a          496 c          420 g          470 t
ORIGIN      
        1 ttatgggtga tcggggagag agtttcaaca gagatttgag tggtcataac gataactccg
       61 tacatttgga aattatcgcc accgacgacg ccaatcgaac tggtggcgaa ctgtgcaggg
      121 ttggcgtaac cgggtacgga aaccggcgag cctttcggct cccccacaca gcccgccata
      181 aatcgcgaat gtgactgtgc aaacgatatg aaaaaagacg cgggcgcgtc tcatatcgct
      241 ccgtaaacat ccgggacgcc aatcccgacg ccagattttg ctggcgcgtg aggaatatag
      301 ccccggataa cagattgagt caacaaacgg tttttagatc cccggaagag aatgcatcac
      361 gcatcggtag atagagcata tattcagcaa gatttagcca tacatctacc cgactgcagc
      421 acgtccggaa acaccactca gggtgtgcgt cattcagcaa ttcagccatt ttacgcttag
      481 tcatcccccg cccttcatac cgttgccgga ggacactaat caatccagga tgctctgcca
      541 gcacctcact tatgactcta tcaatacata acgcctctgc atcagtacaa tgcgccagcc
      601 aggtcttttg cttgccattg atcatctctc gcaaaaacgc ttccagctca ggtttctcta
      661 ttcccgcttt tttcatcctg cgcagggctt cattgacggc tgttttcgtc aattttttgg
      721 atgccagcaa ctggttaaac atatttcccg tcttaccgcc gccaatatac gaccagcgcc
      781 cccacatgcg cagttttccc tgaatccaga cactttccag cgtggtgaga cgaaggtgtt
      841 ccccgctttt gcctgtattt gttgggtaaa tcataaataa ccttcctttc tccagatttc
      901 ttgtgtgcga aaaacacctt ctgcatgcat caggcgtaat tcttctttgg tgtaatcgct
      961 ggtttttacc cgcccgtcga ttaaatcgtg gcatgagcta caggcaatcg ctgcctgcat
     1021 atcgtgtggt tttgtcgctg ttccgcacgt ccccgccagc ctgtaatgcg ccagcacaga
     1081 agtttcggga ttgtgattgc agtagccagg aattctgacg gtgcacatct gcccccgcgc
     1141 cgctttacgt aaatccacca ttacgcaaac tccagtaact gcgcggccac attttcgact
     1201 tcctccggag aggaaaattt acggaacagg atccagttcc acagcacatt cagtacagat
     1261 ttataaacct gctgaaactc ggtttcgtcc atgttcgcaa atgcgataga ttttgccctg
     1321 cgcccacgac taccatcagg ataaatatgc tcggtgtaaa atccggcctg aatggttacc
     1381 cactcgcgga aagcgtcaaa cgactttagc aatgccgtat cccgggttct gcatgtcgca
     1441 actgtattaa ggtattgctc tgcggcatca ctcagggctg gagtgtgttc ccgacctact
     1501 gattcgcaca ggtaatcaac gaagcctgat accagttttc gttcgcgagg cgtgatcgcc
     1561 ccaccgaccg gagtccagta atcaaatccc agttgcagga gtttgaaaaa acgcttgtgg
     1621 aatgcgtagt tacgcacacg cttaaagtct gcgtgtatcc actcacctat tttgatttga
     1681 tgcaaaaaat cgcaactctc cggcgtcgcc gggagaagta atccggaaga ggtttgtttg
     1741 accagttgta tatgcgccat cgtagttctc cgctggcgca gtagaatggg tgttcagccc
     1801 gttatgtagt ataccagaat taatgccaat a
//
LOCUS       DAJTSI010000316         1829 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000316 DAJTSI010000000
VERSION     DAJTSI010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.324"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            174..478
                     /locus_tag="OO327_004211"
                     /pseudo
     CDS             174..478
                     /locus_tag="OO327_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004952983.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
     gene            647..>1829
                     /locus_tag="OO327_004212"
     CDS             647..>1829
                     /locus_tag="OO327_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6020514.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGGSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AA"
BASE COUNT          471 a          365 c          418 g          575 t
ORIGIN      
        1 ccttttttac gatagccaaa ttacctatac ccatcccggc cctgccaagc catacccgct
       61 atcggcgatg aaaatgcttt tgtgcgcggt cggagtggtc ccgacgaggg ttttacccga
      121 agtcggggcg tatctccgcg ttagcgggcc gtgagggccg cttacgagcg tgtatgagag
      181 acgtacagtg gttttgtccg ctcacaccag tacatacagg tacggaaata aaaccagtcg
      241 aaatgccggt aacgacgatt actcctgtct ctgatgccgg tggactacaa gattttattt
      301 actggcgtcc tgatgcggac agggctggta tggaacctgt ttatgtgata cttaatgatc
      361 ctctggattc cgggcgattt acgcgtaaac aactaaataa aaatatctta aacatgcaag
      421 taattttggt attctcgata caagaataaa tagtagtaac tactatagct gcaaataatg
      481 ttaaaaacat ttctatgatc atctttacat ttttcatgat tgacaattgt ttttggttaa
      541 aaaaagacaa ctaatgagtg tgataatgca atactttgag tgattatata atgataagta
      601 aacaggtttg cgacaattgg ttatcagtat tacagaagga ttaaatatgt cattattcac
      661 agaacctaag gaaattgaaa gattaccctc tgaagagatt gaacgattat atcccgtcct
      721 gcgatacagg gtctttatca gcattttcct cggatatatg gggtattatt ttgtccgaaa
      781 caccaccagt gtgttatccg gtgtgctgca catgtctgca accgagatcg gcattatctc
      841 ctgtgccggt tttttatcct atggtatatc aaagtttgtt tccggactta tttctgacag
      901 aagtaattca aaagtatttt tgtctcttgg tttattcctg tccggactgg taaattttct
      961 gataggatat attcccggga tcatcacatc cgttacgtta ttttcaacaa tgtatttact
     1021 taacggctgg atccaaggta tgggatatcc tcctggtgcc aaaacactgg tgttctggta
     1081 tgaacatcgg gaaaggatca cctgggccac actgtggaat ttatcgcaca atgtcggggg
     1141 agctctggcc cccgttctga ttggtttcag ttttggtttt ttcggcgaca gtgctctgga
     1201 tcatgcccgg gccgcattta tcttcccagg agtactgtgt atggcaatga gtgtgctgat
     1261 ttactttatt caggtggaca gacctgtatc agtggggctt cctcccattg aagaatggaa
     1321 aggtaacgtc gtttcacacc cggctaaagg ccgtgaacaa ggtccgagac tctccattcc
     1381 tgatatcatc agaaaacata tcatccgaaa taataaactc atttactgct gtatctatgg
     1441 ttcgtttgtt tatattctga gatacggtat tgtttcctgg gctcctaagt ttttaagtga
     1501 cagtctggat gtcggtggta aggatatggg aaaactggca tcaatggggg ggggttctgt
     1561 ttttgaaatc ggtggtgtgg ctggtatgct tcttgccgga tacctgtctg tcagactgtt
     1621 cagaaactca aaaccattaa caaatacttt atttctggca cttaccatta ttttattaat
     1681 cgcgtactgg tacgtgccat caggcaatga atatctctgg ctaaactata caatccttat
     1741 tctgttgggg ctggcagtgt atggtccggt gatgtttatt ggtctttatt caatggagct
     1801 tgttcctaaa gaagctgccg gagccgcat
//
LOCUS       DAJTSI010000317         1826 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000317 DAJTSI010000000
VERSION     DAJTSI010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.325"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(208..609)
                     /locus_tag="OO327_004213"
                     /pseudo
     CDS             complement(208..609)
                     /locus_tag="OO327_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1038..1826
                     /gene="virF"
                     /locus_tag="OO327_004214"
     CDS             1038..1826
                     /gene="virF"
                     /locus_tag="OO327_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCR6020515.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          570 a          330 c          340 g          586 t
ORIGIN      
        1 caagggaata tagtttgtgt aagcatattg cttccttatg ttatatttta gtaaagaatt
       61 tattttttac attatagtcg gctaaatgaa gtttactgta aaaaaacgat acttttttcg
      121 taagcgatga gatgtggagc cagaaaaatg gctgcgttac gtcattgaac tgtccttgac
      181 ccacgatatt cagacagtcg ttgtatctta agttgacatt acccgctgac gtcgatattc
      241 ccttggtgag agataaccca acgcactatg cggatggttt tcattgtaat gctcgatcgc
      301 cactgcaagg ttatgcaatg ccgttcttgg tttcggcatg aacgcgatgc agtcttcctt
      361 catcgttttc atgaaccgtt ctgctattcc attactctga gggctactga ctgccgtcgt
      421 gcagggctcc agattcaact ctctggcgaa ctgccgtgtt tcatgagccg tataagccga
      481 accgttatcg gccactggat tggccgttcc ggtcctttat cgccatatcg ctcctgacat
      541 gacatcctgc accgttgctt tgtcataatc tcccgtgcct gctgcccagt ctatggcttc
      601 acgatcgcaa tatggaacgt aacgctgtca tcgggagaat cgtcaatgcg gcattcggtt
      661 aacgcttacc aatatcaaca ccaaccggat ttgtggagcc tccagtctga aggggcttta
      721 tgcgttccgt ataggatatt atgatgctgg agttttgcga agctccactc ctttccgaac
      781 atttacacag atattgccta agaaaagtga aaccataata tggttatagt ccctttcagt
      841 gcaaatactt agcttgttgc acagagaaat agaagctgca taagctcttt cttcaaaaaa
      901 tgtaaataaa gttaaatata ggaaaaatta cttaatctat cttaataacg gaaagatttt
      961 gtatacaatc actgttacag aaatttctta gttactctgt aaacactaaa tatagtttgg
     1021 tatattctgt tgaatttatg atggatatgg gacataaaaa caaaatagat ataaaggttc
     1081 gcttgcataa ctatattatt ttatatgcaa aaaggtgttc aatgacggtt agctcaggca
     1141 atgaaacttt gactatcgat gaagggcaaa ttgcttttat agagcgaaat atacaaataa
     1201 acgtctccat aaaaaaatct gatagcatta atccatttga gattataagc cttgacagaa
     1261 atttattatt aagcattatt agaataatgg aaccaattta ttcatttcaa cactcctatt
     1321 ctgaggagaa aagggggtta aacaaaaaaa tattcctcct ctctgaggag gaggtttcta
     1381 tcgatttgtt caaatctata aaagagatgc ctttcggcaa aagaaagatc tatagtttag
     1441 cttgcctttt atcagctgtt tctgatgagg aagctttata tacttcgata tcgatagctt
     1501 cttctcttag tttttctgat cagataagga agattgttga aaaaaacatc gagaagagat
     1561 ggcgtctttc tgatatttca aataacttga atttatcaga aatagctgtt agaaaacgat
     1621 tggagagtga aaaattaaca tttcaacaaa tccttcttga tattcgcatg catcatgcag
     1681 caaagctttt attgaatagt caaagctata ttaatgatgt atcaagactt atcggaatat
     1741 caagcccatc ttattttata aggaaattta atgaatatta tggtataact ccaaagaaat
     1801 tttacttata tcataaaaaa ttttaa
//
LOCUS       DAJTSI010000318         1815 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000318 DAJTSI010000000
VERSION     DAJTSI010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.326"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..217)
                     /locus_tag="OO327_004215"
                     /pseudo
     CDS             complement(1..217)
                     /locus_tag="OO327_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            201..459
                     /locus_tag="OO327_004216"
                     /pseudo
     CDS             201..459
                     /locus_tag="OO327_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(568..1146)
                     /locus_tag="OO327_004217"
     CDS             complement(568..1146)
                     /locus_tag="OO327_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020516.1"
                     /translation="MKVSFKSLGYIFHDIYNKKYTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1567..1815)
                     /locus_tag="OO327_004218"
                     /pseudo
     CDS             complement(1567..1815)
                     /locus_tag="OO327_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          481 a          420 c          321 g          593 t
ORIGIN      
        1 atatcagctt ttcctgcctg gattcggtca tccatttatc gctgtgctgc cacaggaagc
       61 gagttccctt tgcctgacga cgactttcaa caggaagatg cggatgttca ttctggcggg
      121 ttttatccac cacctcaccc ggttgtttcg ccacatgaaa gcgatcgaag gctattttct
      181 caaccgcatt gggtaagtgg atgcgcgctg ctcttagttt gctgagccag tttagcattg
      241 ccttctacat gaccgatgcc tgagccggta taccgaactt tattgtcctc aaccagtcat
      301 gttataagca agaaatgcac tcagcggata gagcgacata atctgaacct gcggacacat
      361 cttaagcggc ttacccgcaa aaccatatgc ttctcaaagt ctgatgatat gcactacaag
      421 atcatcggct ggtatctgac aatcaaccac caccactaaa tctgcgtcac gacctgcaac
      481 tgagtctggt tgaacaggct gaggccctgc tgaagcaacg cgaacctctc gactcttgtt
      541 ggattttacc aaatagtttt tctaaaatta aacacattta tataagttct ttgctgaaca
      601 aaacgcagcc cgccatcctt tttcattcat aaattcagca atggcagata aaactttttc
      661 atcaagcata taaaaaataa tgccagtaga ctcaatatgc ctcatacaaa taaggccgca
      721 catatccgta atatatttgt caatgctgct accatttgca tggtaatatg tcatgctatt
      781 atctgatgat tttacatatg cagtatgaca tatttttcct tcacgattaa caaaactgac
      841 aaaaccactt tcaccaggag taatatataa agatgaattt aacgtgcgct cactaatatt
      901 cattaattgt tgaaattcaa tggaatagtt ttcagttaat gtatctatta tcttggcttt
      961 gtctttcttt gttatctcat tgtctttttc ggcaagaaaa attgccactt tatgacatgc
     1021 attcagctcg ttaattttac ctgataaaac tgcttttctt accacatcgt tgaactcatc
     1081 gatagtgtat tttttattat atatatcatg aaaaatatag cctaaagact taaacgagac
     1141 tttcatttta tattctcctt aaatattaac aggatatata tatcacattt tcctctatat
     1201 tttcagaaac cagctatcat tttccccatt actggtacgt cgcttcagat atttccgcag
     1261 caagcgataa ccctgcgcac tatgaggatg ccattcatta taatgtttga acaccttcgc
     1321 gagactactt tactgctgtt aacacgtcag gttcgaacat aacactgatt taatcatatt
     1381 tcatcgtttt gacgaagttt tctgccattc cattttgcac cgccgtgtgt ttgggggcaa
     1441 ggccgaccat ctgtgtttca ttcgccagat ataagtgatg agatggcatt ctccttcctt
     1501 cacggcagaa gcattttgac cgtttagggg atgcggacga ttttcatgcc tgctaagata
     1561 ctttcgttac tgatgctgtc cgttttccac catttcatca tcactgattg gcgttgtgtt
     1621 tttggtcagc actccagctt tacgtttctc ttttaaccgg taactctctc ctttgatatt
     1681 cagcgtgtag cagccgatct aggatcgctg ttgccagcac gtgatctccg aacatttctc
     1741 cccagtctgc gacccctttg tttgacgtca gtatgatgct cgctttttca tatcgacagt
     1801 tcatcgatag gaatt
//
LOCUS       DAJTSI010000319         1809 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000319 DAJTSI010000000
VERSION     DAJTSI010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.327"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            173..1198
                     /locus_tag="OO327_004219"
     CDS             173..1198
                     /locus_tag="OO327_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6020517.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVSNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1266..1809
                     /locus_tag="OO327_004220"
                     /pseudo
     CDS             1266..1809
                     /locus_tag="OO327_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          421 a          421 c          462 g          505 t
ORIGIN      
        1 caccattgcg gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga
       61 aatcgtgcgc aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa
      121 agacgtttcc caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa
      181 gatgatgata gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca
      241 tgctggcgcg acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga
      301 tggattacct aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga
      361 tgtttgtcgt ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc
      421 actcacagca aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg
      481 taatacgacc tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac
      541 ttattacgac ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga
      601 actggatggc gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga
      661 ctatttcaag gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc
      721 agcgaaggca ctggaatctg gtcgctgcga tacgctggct tcggatcagt cgcaactgta
      781 tgccttgcgc atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc
      841 taaagaaccg cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg
      901 ctggacgctt ttcgccatgc taaatgctga agagatgggc atcaattccc agaacgtcga
      961 tgaaaaagcg gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga
     1021 ttacggcaag gatctgaagt tggataataa atgggcctat aacatcatca aacaggtgag
     1081 taactactcg gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg
     1141 tgggcaaaat aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt
     1201 cggtgtgagg taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg
     1261 tttctatgcc tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg
     1321 ttcgtgcctg gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt
     1381 tatttcaaaa cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc
     1441 tggatcgcgg cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca
     1501 cctacggacg cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta
     1561 ttgtgttcgc ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc
     1621 tgctacgaaa gctttccaca atttatattg agatcttccg taatattgca aatcttcttc
     1681 tggtactttg ccgtgttgcg caatttgccc ggaccacgcc aagctgtgag cgcgtttgat
     1741 ctggcctttt tgagcaatcg tgggctttat attccgtcac cgcagctggg agacggattt
     1801 attgcgttt
//
LOCUS       DAJTSI010000320         1801 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000320 DAJTSI010000000
VERSION     DAJTSI010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.328"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(84..1688)
                     /locus_tag="OO327_004221"
     CDS             complement(84..1688)
                     /locus_tag="OO327_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6020518.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSISLRT
                     KDRRTAMAYSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDL
                     FNDIDRQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLRVRDVLQQLPRNRKQRFKDVPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVAAQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          446 a          457 c          410 g          488 t
ORIGIN      
        1 cccttgacaa gcagagaaaa ctatgagtca aaaacatgac cacacaggtc tgtagtaagt
       61 gtcatcctag actatggtct cctctaggtc tccttataaa catcagccat cacattgacc
      121 agcctatcaa cctcgacctc tttagcataa cggtcgtacg taatagaccc tgaactatgc
      181 cccatgatcg cctgtgcata cgccaactcg accccatgtt gttttaactg ggccgcaact
      241 gtatgtctaa acgagtgaaa cgctaagccg ctacggtcta cgaggacttt aggcaacaac
      301 gtccgattaa accatttact cacctgctca ccgtaaccgt tcttcatgag acgcagacca
      361 tcgaacacca tagcgtcatc accgtcagcg cctctacggg tctctacaag cgccataaag
      421 tcagccaata taaaaccata ggccccatcg accagaggca cacagcggtc actatgagcg
      481 ttcttgatgc tcttaccggg tagactacta tcgtcttcat tgatatgaat ataaacggtt
      541 cccgcctcag tggtcctgac gtctttgacc tgtagttggg ctatttcatt gagtctcgcc
      601 ccagtgatag cagccaaggc ggtaacataa taatgatacg gcttaccaga ggtcttctga
      661 gagtatgcct tagctgcgac cagtagttgc cccacctgct ccgtagagaa ggcgttacgg
      721 gcccctgagg ctttacgctg agggacttta agctccagac cctcagtcat gttcttttta
      781 atcaagtcat tgcgcacagc ccacctaaag acagcagcca tcttaataag atacttgttg
      841 ttgatggtaa cgacatccag acaatctgtc ttatcttcac ggctcaacag gtcagcaagt
      901 gggacatcct tgaagcgctg cttacggttc ctaggtaact gctggagcac atcacggacc
      961 ctaagcatgt cagccctgtt agcgtctgca ttgagaccca gatggtcgaa tatttcgatt
     1021 aacgcagcgt gtgacgcctt gttttccctt aaggttgcag gcttccagtt ctgatagttc
     1081 tccgcttcgt attgttcaaa gaggctcgct atggtaatgc taggcgtaac ttctggtttt
     1141 acttctggag cattaacaga tagggacaca gagtctgctt ggttatcttg cctatcaata
     1201 tcattaaaaa gatcgacgta atctatcaga ggctgactgt ctcccgcttc gatacgcctc
     1261 atacaggctt ttagcacctt cagggcctca ttgatgtaac gatgctggtc aatgcttagc
     1321 ggctcactag ccagagcatc ggtaaggctc tcaccaagtt ctccgtattg gtcccggtag
     1381 atatccctca tgtcaggctc aaagagatct gaacggccca tagacagttc acactcagcg
     1441 atgtccttaa ggtgttccct catttcctca taggtagcgt tgggtctgtc tgcgtgtatt
     1501 gccttgagtg ctgcctttat gtgccttgag taagccatag cggtgcgtct atccttggtc
     1561 cgtaatgata ttgaggtcat tctatcattg tttgattgtc tgaccctaag acggaagtag
     1621 tagacgccgt tacgctggta gagatagtag actgctggaa gtttgtgact actggatagc
     1681 tgaatcatat gtctgcatgc cctgagaggg ttacacagtg ggttacagta agtaactctg
     1741 caaggaggct taaagccgta tcccttagga ttcagtaggt tggagtactt taagaaaggt
     1801 c
//
LOCUS       DAJTSI010000321         1733 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000321 DAJTSI010000000
VERSION     DAJTSI010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.329"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(9..218)
                     /locus_tag="OO327_004222"
                     /pseudo
     CDS             complement(9..218)
                     /locus_tag="OO327_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            complement(268..447)
                     /locus_tag="OO327_004223"
                     /pseudo
     CDS             complement(268..447)
                     /locus_tag="OO327_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            971..1255
                     /locus_tag="OO327_004224"
     CDS             971..1255
                     /locus_tag="OO327_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HCR6020519.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHLLPVPR"
     gene            1255..1530
                     /locus_tag="OO327_004225"
     CDS             1255..1530
                     /locus_tag="OO327_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR6020520.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          476 a          435 c          407 g          415 t
ORIGIN      
        1 ctcactcctt atttacccgg gaaatacttc tccagcaatg catacaggat ggcagcggtt
       61 tcagcatcca ttatgccgtc ataatttttc tgccggaaat gaagctgaaa agcgcggaca
      121 agcgccttat atccgtcatc cgtctccaca ccagaaatac tgtatccata ggtcttcagc
      181 cgtgacacga tatcaacctt atccggaatg ccctgcgatt caaaaaataa atatttagtc
      241 agtaatttta tgtttttcct gaaaaaatta ctctttttcg aactccagtg agcgttcacg
      301 actcatcagt gtgaaaatga tggcaaaaac agcagacagt atcagtacga taaatgtggc
      361 cccccatccc agataatcga caacaagtcc cattcccagt gtcgcaacaa ttgagccgaa
      421 gatataggaa aatgtgcctg acagccctaa cagaataagt ttacgcgtaa atcgccctga
      481 atccagaaga gcattaagta tcacataaac aggttccata ccagtcctga ccgcatcagg
      541 acgccagtaa ataaaatctc gtagtccacc ggcatcagag acaagagtaa tcgtcgttac
      601 cggtatttcg actggtttgt actggtgtga gcggacaaaa ctactgtacg tctctcatac
      661 acgctcgtaa gcggccctca cggcccgcta acgcggagat acgccccgac tgcgggtaaa
      721 cccttgtcgg gaccactccg accgcgcaca gaggcatttt catcgctgac aggccgcatg
      781 gcctcgcaga gccggagggg gactggtgat attcggacga ccgtaccggc ttgcgccggg
      841 cttcggcggt tttacacctg tgctacatgt aaccaccgta tgccgcctta tatgcgctga
      901 ctcgcattat ttgccttgtt ccgtctgaaa gtaatcacta tgattaaata tgattaacaa
      961 ctaatcgggt atgcaaatga aaaacaatac cgcacaagca acaaaagtaa ttaccgcgca
     1021 tgtgccatta cctatggctg ataaagtcga ccagatggcc gccagactgg aacgctcccg
     1081 gggctgggtt atcaaacagg cgctttctgc atggcttgcc caagaggagg agcgtaatcg
     1141 cctgacgctg gaagccctgg acgatgtgac atccggacag gttatcgacc atcaggctgt
     1201 acagtcctgg gcggacagcc tcagtactga ccatctgtta ccggtgccac gctgatggaa
     1261 ctgaagtgga ccagtaaggc actttctgat ttggcgcggt tatatgattt tctggtgctg
     1321 gccagtaaac ctgctgccgc cagaacggtg cagtccctga cacaggcgcc ggtcattctg
     1381 ttaactcatc cacgtatggg agaacagttg tttcagtttg aacccaggga ggtcagacgg
     1441 atttttactg gcgagtacga aatccgttac gaacttactg gccagactat ttatgtattg
     1501 cgcctgtggc acacacgaga gaacaggtag attgggtgtg gaggcaactt taacatagcc
     1561 tgcaggtatt aattctggag cattgttatc aggccagata ttttgcattc cattaaataa
     1621 tcttgtagga gataaaataa attacgatga aataacgaaa attaccgctc gtgactggcg
     1681 gcaatatcgc gctccgggct ggcaaataac ccatcagaaa cgttattgcc aga
//
LOCUS       DAJTSI010000322         1731 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000322 DAJTSI010000000
VERSION     DAJTSI010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.330"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..259)
                     /locus_tag="OO327_004226"
     CDS             complement(<1..259)
                     /locus_tag="OO327_004226"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020521.1"
                     /translation="MARSAKPRKRKPAPQRSKLLRYVVKLHEDDFFDEEEAEVLRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARC"
     gene            complement(1012..1485)
                     /locus_tag="OO327_004227"
                     /pseudo
     CDS             complement(1012..1485)
                     /locus_tag="OO327_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
     gene            complement(1413..1731)
                     /locus_tag="OO327_004228"
                     /pseudo
     CDS             complement(1413..1731)
                     /locus_tag="OO327_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          403 a          478 c          401 g          449 t
ORIGIN      
        1 tgcagcgggc aatttccgga tacccttcgt catcaacacg tacaaaccag aagaccagtt
       61 ttttgtttcc ggcatccaca aagaacggaa tattcaggtc tgcgcaacac tcaacggcat
      121 cgtcaaaatt atcaaagcgc agaacctcag cttcttcttc gtcaaaaaaa tcatcttcgt
      181 gaagttttac gacatagcgg aggagtttgc ttctttgagg tgcaggtttt cgtttgcgtg
      241 gtttagctga acgggccata taactacctg aaagacaatg atattgtctg tttttataac
      301 ggtaatcaca ggccatgaca agccgcagcc gtcaggccgc ctactcgggg cggtttagaa
      361 cgcttattta gtaccagatc ttacttataa taaaggggct tggcagtttg gataaactat
      421 tgtccttatt ccactaatac aaactttttg taaagtatat taaatatact tcatttcata
      481 ctgaaatccc cggaagtatt caatcaaaca attacaccat aaaaggaaga gggggcccca
      541 attaagttgt caagcatgtt tactgcgcaa tcgcttggtg ctccgtccct gtaccccgca
      601 ttggtactct ggcgctcatg gggcttccac ttgcgcttct cccttcacat cggggtgaca
      661 ggtccacgtt ccccacaaga gcctgaatcg acttcacgcc gcttttatgc cggatgccgc
      721 gcaggcagta aacatatccc acaggaacga ctccaccatt gttttgacat catctatatg
      781 ctttcgacac atcatcagcg gttcactttc gttcgtctct tcgaaccata cctgatcggc
      841 tcacgtccga ccttttccca tctcgctcac caccacatct cttaaatgca gcagcagtgg
      901 atggtttggt gcctgcgcct gcacgccgac accaaggggc cttccctcat ctcttgtgct
      961 gcatggctgc acagggactc acgtccccag gacgcgcctt cgtggcacac agtcatcggc
     1021 atagcaacag taagcaacgg caggtttcca ttgccagttt tctttgacag cagtacttcg
     1081 gcctcgctgg atactgtgat tccagtacca gcgatccttt ctggcttttc cactcaggta
     1141 gcgtttattc agatactggt cgaactcatt cagtatgatg ttcgacagca acggcgagat
     1201 cacaccgccc tgtggcatac tttcactgac cgcccggaag agaccaacat caacacggcg
     1261 ttttccacag cagtctcata aaacgtgtat cactgatcct gcggcagacg gctttcatca
     1321 gcagccggtg atgtactgtg tcgaagtagc tggacaggtc accttcaata acccggcgcc
     1381 ccgtgtttcc ccgcagtcgg tgagctgtaa tagtacgtga tgtctcccgc ccacttctgg
     1441 ttcgggccac tggcgtaaaa atcctgcgtc agcagatttt ctgatacagg caggccgtgt
     1501 gcacggtaac tgatcgggct gaacttctgg gaggcttttg cgctcagttc ctgacggcgc
     1561 agactggctg ccacggtttt tacgttaaag tggtaaccag agcacacagt tcatccgtca
     1621 ggcgtggggc accgtaacgc tggactgggt aaaagccttg cggacaacgc tgtcgcagtg
     1681 ctgacggaac tgcggacgtg tgtttatcct tgtccgccgc tgacaccacg t
//
LOCUS       DAJTSI010000323         1719 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000323 DAJTSI010000000
VERSION     DAJTSI010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.331"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            671..>1719
                     /locus_tag="OO327_004229"
     CDS             671..>1719
                     /locus_tag="OO327_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR6020522.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIP"
BASE COUNT          429 a          431 c          513 g          346 t
ORIGIN      
        1 tgtgcccgga gttcagggcg ggcatggatg cttaaatgaa ccgcgagtct gtctggaata
       61 ttgaaccggt aactcacgat gagaaaccca acaatcccac cgggtgtgac ggtggagaac
      121 ctgagcggca gtgacctgcg gcatgcccgc agggtgatgt aacccgctga caacggggat
      181 tgaggcgaga tcactaagcc gagatgatcc tcaaggttaa gtactgaaag gctgaagaac
      241 atgaacccgt taatccgcct ctgtgggttg aaaacgtcac cacggcctac gtgatctgac
      301 aggccgtgca ggaggaactg gcagtgatac gtaagcactg ccggtcgaag gtgttttgac
      361 atgtatgcga aacaccgggg cagcagcgtc tatcacactt gcgttgctga cttctgccaa
      421 cttgcggcaa gcaaggataa agagtgcgac gggcagcctc ctcagtatgc ctgagtccag
      481 gcaggtaaac cggggaaggt cagcgacgga tgttaagggg gcatggctcc gatgacgcgc
      541 tggctggcgg agcttccgta gtagtccgcg atggggaaag cccattacat ggcgaaggga
      601 agcagtttga atgtgtttgc gacgtgaatt aactgaccta acgaggtgaa gacctttgat
      661 aatcagcgaa atgcaacgca agcttgccac atgggcagcc accgatccgt ccctacggat
      721 tcaacggctg ctgcgtctga taacacaacc agaatggctg gctgaagcgg cgcggatcac
      781 gctttcatca aagggggccc atacccccgg cgttgatggc gtgaacaaaa caatgctaca
      841 ggccagactg gctgttgagc tgcaaatcct cagggatgaa ttactctcag gccactacca
      901 gcccttgccc gccagacggg tttacatccc taaaagcaac ggcaaactgc gaccactggg
      961 tatccccgcg ttgcgggatc gtattgttca gcgggccatg ctgatggcga tggagccgat
     1021 atgggagagt gattttcata cgctctcata tggcttccgg cctgagcgca gtgtccacca
     1081 cgcgatccgc acggtgaaat tacagctcac agactgtggt gaaacccggg gacgctgggt
     1141 gattgaaggc gacctgtcca gttacttcga caccgtacat catcgactgc tgatgaaggc
     1201 tgtacgccgc aggatcagtg acgcacgttt catgactctg ctgtggaaaa ccatcaaggc
     1261 gggacatatc gatgtcggtc tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt
     1321 tatatcgccg ctattatcga acatcatgct gaatgagttc gatcaatacc tgcatgagcg
     1381 ctacctgagc gggaaagcca gaaaagatcg gtggtactgg aataacagta tccaacgggg
     1441 ccgaagtacg gcggtcagag aaaactggca gtggaaaccc gcggtggcgt actgccgcta
     1501 tgccgatgat tttgtcctca tcgtcaaagg caccaaagca caggcggaag ccatcaggga
     1561 ggagtgtcgg ggtgtgctcg aaggcagtct gaaactcagg ctgaacatgg ataagactaa
     1621 aatcacccat gttaatgacg gctttatctt tctggggcac aggatcattc gcaaacgcag
     1681 tcgttatggc gagatgcgag tggtctcaac gatcccgca
//
LOCUS       DAJTSI010000324         1717 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000324 DAJTSI010000000
VERSION     DAJTSI010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.332"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(110..577)
                     /locus_tag="OO327_004230"
     CDS             complement(110..577)
                     /locus_tag="OO327_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR6020523.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(643..882)
                     /locus_tag="OO327_004231"
     CDS             complement(643..882)
                     /locus_tag="OO327_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HCR6020524.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1017..1622)
                     /locus_tag="OO327_004232"
     CDS             complement(1017..1622)
                     /locus_tag="OO327_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6020525.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKVVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          550 a          377 c          305 g          485 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta
       61 ctgagagaaa aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca
      121 atgggaaaac aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac
      181 cgttttcacg aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc
      241 aaaaggtcat tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa
      301 cagttaacag cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca
      361 cgagcaaaac gctcccggct gcaaccagat tgggtttttg atgctgtaaa atgagtgcca
      421 aaaaaataag cacggttaac accagtaaat aacgatggct aataattctg agtaatatat
      481 ccccccatga aataaaaata agaaagagcg caacaaataa aagatcgact cttagtaacc
      541 atattgctgc cattgaaaga ctccatgtac catacaaggt tgctgccaga catattcctt
      601 tacgtctgac agcctctaga gagttttttt attttttata agttacggat tactcgttcc
      661 ggaaatcgtt acctgttgaa ttgctgcagc gatagcgtcg aatgcatctt taattgcgcc
      721 caggaagcct gagttctgat cgccaaagat gaaagcaagc agagttgcca tagcaacacc
      781 aatcaatgca tattcgattg cagtaacacc acgttcatcc ttcgcgaaac gcgcaaagca
      841 ctgtttagtt ttaccatagt acttagtaat aaaagtattc atgttttcct cgtatattat
      901 tatttaaatt caaaccacca gcttcatctg cgtgtcaccg gtatcccata tttttgggat
      961 gtggctataa ttatctattc ccgctattta gtcttcaaaa aaaattacat ttaatatcat
     1021 ttatcttgat taagaaatag taagactaaa aaaacaagcg tcgtcagcca gcacactgct
     1081 aacacggtaa gaacgctaat aaaaacactc cactgcttcg ttctatacca ttcaatattt
     1141 ttgtcagtat gttttattgg gagcagctct tcagtagaga attcctggct gttatcattc
     1201 tcaggaatgt ttgcatcagt gtttacttcc ctggcctcgc tatcgttggt atagctacta
     1261 ctgtcttcat cactgtcacg ctcattctca cagacatgtt tctcgacctt tcccagatat
     1321 ctgacgactt ttcgcggaat ggttttaatt agagaactcg acaaacctgc gacgtctaat
     1381 gcctttctaa gcatgtaaat ctgcccataa taactgctgt cgcttaccga acctttctgc
     1441 tcccgccaaa cctcattgat aatatgttgc ttatcggatt ctccctgtag taaaaggagt
     1501 aaaaatcgat agccgttttc agacagattg attacattgc ctgtactggt ggtgatacgt
     1561 cgagacgctg ggtaaaactt aactccctgc cctaaagaaa aataaaaacg gtcatcgctc
     1621 ataagtaccg acccctgttt tcttcttcac atgactactc attacaactt tgtatgaagt
     1681 aagatgcaaa atcatattgt ttacattaag ttaagca
//
LOCUS       DAJTSI010000325         1663 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000325 DAJTSI010000000
VERSION     DAJTSI010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.333"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            339..1397
                     /gene="rfaQ"
                     /locus_tag="OO327_004233"
     CDS             339..1397
                     /gene="rfaQ"
                     /locus_tag="OO327_004233"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR6020526.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            1394..>1663
                     /locus_tag="OO327_004234"
     CDS             1394..>1663
                     /locus_tag="OO327_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR6020527.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKM"
BASE COUNT          431 a          355 c          389 g          488 t
ORIGIN      
        1 tttgacttaa atagcaattt acccgccaat tcttgttgaa catctatact ttttttgtat
       61 ttttatgcga ttcattgatt tctgctgtac gtgcaggaag gaatcgcaga gcaacagtga
      121 aagaatatgg ttgctaatga ttttatttga ttgatttctt tattgaaaat caacaatgtg
      181 cttaaaaatc attcgggtcg gattgccgcg aaagaagtga tacactagcg cgtcaatatc
      241 agtgcggtat cagtattcag gtagctgttg agcctggggc ggtagcgtgc tttttctgct
      301 tcaccttaat cggataatct caacaaaaag agttacttgt ggataagcca tttcgaagaa
      361 ttttgctcat taagatgcgt tttcatgggg atatgttatt aactactccc gtcattagtt
      421 ctctgaaaaa aaattaccct gacgcaaaaa tcgatgtgct gctttatcag gacaccatcc
      481 cgatcctgtc tgaaaatcca gagattaacg cgctctacgg cataaaaaat aaaaaagcaa
      541 aagcctcaga aaaaattgcc aacttttttc atctcatcaa ggtattacgt gccaataagt
      601 atgaccttat cgtcaatctt accgatcaat ggatggttgc tatactggtt cgcttattaa
      661 atgctcgtgt gaaaatttcc caggattatc atcatcggca gtctgctttt tggcgtaaaa
      721 gtttcaccca tttggtgccg ttgcagggtg gaaatgtggt ggaaagtaac ttatccgtgc
      781 tgacaccatt gggacttgat tcgttggtga agcagacaac catgagttac ccgcctgcaa
      841 gctggaaacg tatgcgtcgc gaacttgatc acgctggtgt tggacaaaat tatgtggtta
      901 tccaacctac ggcgcggcaa atcttcaaat gctgggacaa cgccaagttt tccgctgtga
      961 ttgatgcctt acatgctcgt ggttatgaag tcgttctgac gtccggccca gataaagacg
     1021 atctggcctg cgtcaatgaa attgcgcagg gatgccagac gccaccagta acggcgctgg
     1081 ctggaaaggt gaccttcccg gaacttggtg cgttaatcga tcatgcgcag ctgtttattg
     1141 gcgttgattc cgcaccggcg catattgccg ctgcagttaa tacgccgctg atatcgctgt
     1201 ttggcgcaac agatcatatt ttctggcgtc cctggtcaaa taacatgatt caattctggg
     1261 cgggagatta ccgggaaatg ccaacgcgcg atcagcgtga ccgaaatgag atgtatcttt
     1321 cggttattcc ggcggcagat gtcattgctg ctgtcgataa attactgccc tcctccacga
     1381 caggtacgtc gttatgatcg ttgctttttg tttatataaa tattttccct ttggcggttt
     1441 gcagcgcgat tttatgcgta ttgctcagac agtcgccgcc cgaggtcatc atgttcgggt
     1501 ttatacccag tcgtgggaag gcgaatgccc tgatgtattt gaagtgatca aagtgccggt
     1561 taaatcgcat accaatcacg ggcgcaatgc ggagtatttt gcctgggtgc aaaaacattt
     1621 acgcgaacat cccgtcgata aagtcgttgg attcaacaaa atg
//
LOCUS       DAJTSI010000326         1653 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000326 DAJTSI010000000
VERSION     DAJTSI010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.334"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            454..1302
                     /locus_tag="OO327_004235"
     CDS             454..1302
                     /locus_tag="OO327_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6020528.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPTMTTAHNRAQAVFLC
                     VKHSHIQIMVGRAGQPQGWPVSVVTGCSNPVRLTTHEIATSGGESFKLTIEAAIMATI
                     LTLSHPDATIENGRAVTTSVAVAEFFRKMHKNVIQKIETLECSPEFNRLNFKPVTYTD
                     AKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMI
                     PGDGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVE
                     QLLSLK"
     gene            1319..1600
                     /locus_tag="OO327_004236"
                     /pseudo
     CDS             1319..1600
                     /locus_tag="OO327_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          463 a          380 c          393 g          417 t
ORIGIN      
        1 agcattatat agttctactc tggcgagata cgttggcata ttcatttcct tactggttgt
       61 gtgagaactt cagtaaagat accaccaaag cccggaagtg gtgaaataaa accgggcaca
      121 acacgaaggc gcatttccgg tatccataaa gagtcggtct tgtctgtgaa atttaaatgg
      181 tgggagtgcg cttccggttg taaataacga cattgctgtg tgaagtacca gttggcggca
      241 tcggtttaat tgctggctga tgtccgccct ttttaaagtg aattttgtga tgcggtgaat
      301 gcggctatgc gcacgcggca cagttaaaag tcatgttagt ccttattggt ttgggtggga
      361 aagccaactg taattgttaa ctggttaacg tcacctggag gcaccaggca ccgcatcaac
      421 aaagttcact tcggtgatga aaggaaagag aaaatgttga atgtagctat tgaaaaccag
      481 aacgggtgga attatagtgc acctgcacct cataaaacgg gtgccgggat tgctaccccg
      541 acgatgacta ctgcgcataa ccgcgctcag gcggtttttt tatgcgtcaa gcacagccac
      601 attcagatta tggtggggcg tgcagggcag ccgcaaggct ggccggtttc ggtagtcacc
      661 ggttgtagca accctgtgcg tctcaccacc catgagattg ctacctctgg tggtgagtca
      721 tttaaactga ctatcgaggc tgccatcatg gctactatcc ttaccctttc tcaccctgac
      781 gcaaccatcg aaaatggtcg cgctgtcact acgtctgttg cggttgcgga gttcttccgg
      841 aaaatgcaca aaaacgttat tcagaagatc gaaactctcg aatgctcgcc ggagtttaac
      901 cggcttaatt ttaagcccgt cacctatacc gacgccaaag gcgaaaaacg cccaatgtac
      961 caaatcacca aaaacggctt cgttttcctg gtgatgggct tcactggcaa aaaagccgcc
     1021 gcattcaagg aagcctacat tgctgagttc gaccgcatgg aggccgaact gcgccagaat
     1081 aacaccccac ctgcagacaa gatgattccg ggtgatggtc gcactctggt tgttcacttc
     1141 gacaaattcg gcaatgtcga attcaccgaa accgttcctg atggcgcact ggtctgtacc
     1201 ctggagactt tccggtttta tctggaaaaa cagggatgga ctcttgttaa ccggggcgca
     1261 attaaaaata tgactgtgga acaattactt tctttaaaat aaaaatggag ctacggatat
     1321 gaaccctatg cttgattatt gttttacctc tgcacctcag cagaccataa cacacaccgg
     1381 aatcaaaaat ttgttatgga taatcaaaaa aaacctccag tagaaaggtg atcccgattt
     1441 aattccctgt gcttatggcc tgtgtgacag acaagtaagc cctgataagg cttacactgc
     1501 tgaacttatt tacatgcacg gaaccatgat gaggaaaaag aaacgcaaat attgtagtga
     1561 aatctgcgcc gaaaaagacc agatggcaca cgaactttaa ttaactgact attcgaaact
     1621 gaatttatgc cagcaatggc agggattcgc tca
//
LOCUS       DAJTSI010000327         1644 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000327 DAJTSI010000000
VERSION     DAJTSI010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.335"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..430
                     /locus_tag="OO327_004237"
     CDS             <1..430
                     /locus_tag="OO327_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020529.1"
                     /translation="SPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAI
                     DKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNP
                     KTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
     gene            550..723
                     /locus_tag="OO327_004238"
     CDS             550..723
                     /locus_tag="OO327_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCR6020530.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            823..1644
                     /locus_tag="OO327_004239"
     CDS             823..1644
                     /locus_tag="OO327_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR6020531.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
BASE COUNT          427 a          416 c          420 g          381 t
ORIGIN      
        1 aagtcctgac acaaacgccg ccactgtaaa tcagacactt aaatgcgact caaaagcccg
       61 ggctgaccag cctggtgcat gtccaacaac gtacgagtta tacgaaggtg acgctaccta
      121 cagagctgct atcgacaaag cattaaaccc tgttgggtta aacggaatgt ttggggaaga
      181 cggctatatg gatggtcctg atggtggtgc gtatcctgta aacattaacg gaacgacctg
      241 ggtagaaggt ggcggttgta aagcccacgc ctgcggctgg gactatatcg taacactcta
      301 taacccaaaa acgcataaag tcgttggtta ctattacaac atcgaccctg gttacctgat
      361 ttggtttggt gaaactggcg ttcacgaatt cgcgtacctg gtcagggact acgtaaacaa
      421 aacaaattaa aggcgtaagt tatgcggttc atcttcgcgt tattaattta ttgatatcaa
      481 aagtaaatac ggctctgcca tgatgacagg gacttatatt ttcagggaac caaatacagg
      541 tctggttgta tgtcacgttt attgcccgaa gtccctttct cacgcgagca ggctgttgtc
      601 atcacaacag cttaccgcaa tgtgtttatc gacgatgacc agggaactca tttccgtcgg
      661 gttatccgca acgcagagac tgacgccgga aggttcgcca gtgacggtct tctcagacaa
      721 taaacctcat taatttcgtc catcaggccg cgtcttctcc gggagacgtg gctttttcat
      781 ttattacacc agtaaatctt aaccaccgat aaggagcaaa ttatgcgact agccagtcgt
      841 tttggttatg ctgcaaacca gatacgccgt gaccgtccgc tgacacacga agaactgatg
      901 cactatgttc ccagtatttt cggtgaagac cggcatactt cccgcagtaa acgctatgcg
      961 tacactccca ccatcaccgt actggaaagc ctgcagcggg aaggctttca gccattcttc
     1021 gcctgccaga cacgtgtgcg tgacccgggt cgccgggaat acacaaaaca catgctgcgt
     1081 ctgcggcggg ccggacagat aaccggtcag catgtccctg aaattattct gctcaactct
     1141 catgacggtt catccagcta ccagatgtta cccggatatt ttcgtgccat ttgtaccaat
     1201 ggactggtct gcggtcagtc tctgggagaa ttgcgtgttc cacaccgggg aaatgtggtg
     1261 gagaaagtta ttgaaggggc ttacgaggtg gtgggcgtgt ttgaccggat agaggagaag
     1321 cgtgatgcca tgcagtcgct ggtcctgccg ccaccggcac gccaggcgct ggcacaggcg
     1381 gcactgactt accgttatgg tgacgaacat cagccagtca ccaccgctga cattctgaca
     1441 ccacgacgca gggaggatta cggtaaagac ctgtggagcg catatcagac catccaggag
     1501 aatatgctga aaggcgggat ttccggccgc agtgcaaaag gaaaacgtat ccacacccgt
     1561 gccattcaca acatcgacac cgatattaag ctcaaccgcg cattgtgggt aatggcagaa
     1621 acgctgctgg agagcctgcg ctga
//
LOCUS       DAJTSI010000328         1620 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000328 DAJTSI010000000
VERSION     DAJTSI010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.336"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..464)
                     /gene="cutC"
                     /locus_tag="OO327_004240"
     CDS             complement(<1..464)
                     /gene="cutC"
                     /locus_tag="OO327_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR6020532.1"
                     /translation="MALLEICCYSMECALTVQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDAL"
     gene            complement(478..1044)
                     /locus_tag="OO327_004241"
     CDS             complement(478..1044)
                     /locus_tag="OO327_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6020533.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1260..>1620
                     /locus_tag="OO327_004242"
     CDS             1260..>1620
                     /locus_tag="OO327_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR6020534.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYS"
BASE COUNT          391 a          439 c          386 g          404 t
ORIGIN      
        1 agtgcgtctg atttttgccc tgatgtcagt acccgggcaa tgcccaattc cgcaagatta
       61 ttgagtgtat ataaagggtt agcgcacata tcgaaggcgc gatgaaaagt cactgccagc
      121 ggaccggcag cagccattat tttttccatt cgtggcatat cgacattccc gtcaacatcg
      181 agaacgcccg tcaccagtcc aggaaaacct aattcgcgga ccgtgcgcac atcctcaaga
      241 atggcggcaa actcaccgtc gctgtagcaa aaatcaccac cgcgtgggcg aattatcgga
      301 tgcacaggga tcgtcacccg ctggcgcacg gatttcagta cccccaacga cggcgttaag
      361 cccccctctt ttggggctgc gcataattca actctgtctg cgccgttttg ctgcaccgtt
      421 agtgcacatt ccatgctgta acagcaaatt tccagtaatg ccatttttgc tccttaatta
      481 cgctgactgc tcgctggcaa cgatctcttc aatggaccac ggatgaaact taatagttgt
      541 tttgccatcg gtgaccgcca gcgtcggatt cggtaatcgc tcatgctcac cttttggtga
      601 actggtttta actgagatcc ccggtaggct gagtccttca tcagaaagca atgccagcgc
      661 acgggcattc agcgtttctg gatcacccgg cagaacgatt tcaatatgtt cccaaccttc
      721 gtgtgggtaa cgtttttccc cgggccacgg tagctccaca ttggaaaact gccagtgcgc
      781 aacctgtacc ggttcatgca atttaaacag acaaatcggt ctgccattga tcattttttc
      841 tgacaaaagc tcgccacact gttcaaaacc gcgacgccag cgctcagcgg tgacgttttg
      901 atggcaacgc aaagaaatgt gatcggcagt cagcggagtg atatccaacc ccagacggcg
      961 ggaaagttca tctaatgcgt gggtaaatcg cggtaaatcc gatgcaatat cctgcagctc
     1021 gtcgatagat tgccagttcg ccataatcac tcttcgtctt tcagtaaaag cgttaattta
     1081 ccctgttgcc ctgtgccaac caaccgctga tttcacgccg cttctgatgc aatagtgaaa
     1141 acggcaatac gccacgcgca cgttgctgac gaaaacagcc atttgcagta tactcccgcc
     1201 ctaatttctt taactggtgc gggcaatttt tgctcgcttc atcaatgtaa ggtattccgg
     1261 tgaatattca ggctcttctc tcagaaaaag tccgtcaggc catgattgcg gcaggcgcgc
     1321 ctgcggattg cgaaccgcag attcgtcagt cagcaaaagt tcagttcggc gactatcagg
     1381 ctaacggcat gatggcagtt gctaaaaaac tgggtatggc accgcgacaa ttagcagagc
     1441 aggtgctgac tcatctggat cttaacggta tcgccagcaa agttgagatc gccggtccag
     1501 gctttatcaa cattttcctt gatccggcat tcctggctga acatgttcag caggcgctgg
     1561 cgtccgatcg tctcggtgtt gctacgccag aaaaacagac cattgtggtt gactactctg
//
LOCUS       DAJTSI010000329         1601 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000329 DAJTSI010000000
VERSION     DAJTSI010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.337"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(333..479)
                     /locus_tag="OO327_004243"
                     /pseudo
     CDS             complement(333..479)
                     /locus_tag="OO327_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            533..715
                     /locus_tag="OO327_004244"
     CDS             533..715
                     /locus_tag="OO327_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HCR6020535.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(816..1145)
                     /locus_tag="OO327_004245"
     CDS             complement(816..1145)
                     /locus_tag="OO327_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCR6020536.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1317..>1601)
                     /locus_tag="OO327_004246"
     CDS             complement(1317..>1601)
                     /locus_tag="OO327_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020537.1"
                     /translation="HFVLLNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLN
                     DAVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          435 a          368 c          377 g          421 t
ORIGIN      
        1 agcaatgtct gtcgcacaac aaccacaatg tcacaaataa aaaatgagaa taaccagttc
       61 cctgaaggcg acgtagcccg gtacaagagt aacccacgtt agcgcttggc gacggcggaa
      121 caaagctgat tcgcgacgta agaaagtggc aggctcatat gacggacctc tgtaatgcgg
      181 tatccaaccc gcgaatatca gcatgattca ccgtcggatt tactgctacg tcgccctcag
      241 ggaattaaac ctaaaaagaa aatcgtcaaa attagagatt ccggtttgac acggaaagtc
      301 atatcagcgt caacggagca ccgtattgac gcttatttat tggtgagaac tacgttccat
      361 ggcaggagtt cgtcaacacg gttggagggc cattcaggca gtatgctcag aatatggcgc
      421 agatacgctt ccggatcgat accgttcaga cggcaggtgc ctgcttttcc ggcaacaagc
      481 aggaatctca gcgctgaagc aggaattaga ggtaaaaacg ccctaccggg ccatgaatca
      541 cccggtcatt ggagtagtaa ccaaagcaga tttagccagc atggaacaaa tatctttggt
      601 gaaaagttgg ttacgggaag ctggagcgca caacgtgtta gtaaccagtg cagttaataa
      661 caatggagtt acagaacttt ttgccttgct gcatacagaa gaaggctgtt gttaatttcg
      721 attggttctc agatgagaat gatgacggtg atacacagat aagaatcaat gagcaatgag
      781 ggttgccggg caaccctcat tgaataaaac gggaattact tcgcttcgcc gtttttcatt
      841 tcgcccatag ctttcagctt tttggagata tcgcggcgtt ctttggagag ctcggcattt
      901 ttgatgatgt aatcgtcaac gcgatcttca tagtcacctt tcatgctggc gatgatgccc
      961 tggattgctt caacgctcat ccctggcttg atgtaatcgc tcaggttgtc cagcaacagg
     1021 acgcgcttct ggttgtcacg gatctttttc tcaacgtcct gaatttcacg ttgcagtttg
     1081 ttcttacgac ggaacagacg aacaaattcc agtacgtcct ggaatgaagg cttggtagtt
     1141 tccattttta tacccctgat aatgtgagag tcggattcgt ttaagcaacc gctattcgtt
     1201 agggccaaca ttactcatag ctgccgcaga tgacaatgct tttatccctt ttcactatca
     1261 taccctttat ccttgctgaa tcgaagcagc agcaagatga ttctgaagtt caggaattat
     1321 ttgcgcaagg cccggcaaat cagattcgat agcaactcaa gctgatgtgc cgtttccatg
     1381 ttcagccaca cataaccata gattggtgtt tccacgactt tcaggtcatg gtggttatcg
     1441 agcaactgac gcagctcttc cacagcatcg ttcaattttt ccgtattggc aaaaaccggc
     1501 tgcggattac cttcgtacag cgcatgaaca aggctcagca gtatttgctg catcatgtgc
     1561 tgggtatcac aaagtttttg cgcgttcaat aacacgaaat g
//
LOCUS       DAJTSI010000330         1600 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000330 DAJTSI010000000
VERSION     DAJTSI010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.338"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..360
                     /locus_tag="OO327_004247"
     CDS             <1..360
                     /locus_tag="OO327_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6020538.1"
                     /translation="PPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            361..726
                     /locus_tag="OO327_004248"
     CDS             361..726
                     /locus_tag="OO327_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6020539.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            723..1406
                     /locus_tag="OO327_004249"
     CDS             723..1406
                     /locus_tag="OO327_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR6020540.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGKGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKKRR
                     HFQSKVLQK"
BASE COUNT          453 a          324 c          437 g          386 t
ORIGIN      
        1 ccaccacagg aagagacggt ggtcagcatt gccgttgatc cggaatctcc ggctcagtat
       61 ctccagcgcc agaaaccaca acgggaagag atgcctgtat acacgcgctg ggtaaaaacg
      121 cagaaatgca tgacgtgtgg caatcaggca gatgatccgc accacatcat tggtcatgga
      181 ctgggaggga tgggaacaaa ggctgacgat ttgtttgtta ttccgctgtg tcgtaaatgc
      241 cataacgaac tacacgccgg ggtaaaagat tttgaagaaa aacacggtag tcagttgttg
      301 ttgctgattc gttttttaat gcacgcgaga aattcgggtg ttctgaagtg gaaagcataa
      361 atgaccgaac gcatagaatt tgttttgcct tacccgccaa cggtgaacac ctactggcga
      421 cgtcgtggca gcacatattt tgtatcaaaa gccggtgagc gttatcgccg tgatgtggcg
      481 ctaattgttc gccagcagcg actgaaatta aacctgtccg gaaggctggc gataaagatt
      541 attgcagagc caccggataa gcgccgtcgt gacctggaca atatcctgaa ggcaccgctg
      601 gatgcgctga cgcatgctgg actacttata gacgacgagc agtttgatga aatcaatatt
      661 gtgcgcggtc agctcgttcc tggtgggcgg ctggggataa aaatcacaga actggggtgc
      721 gcatgaataa ccagtattta cagtttgtgc gtgagcagct cattatcgcc accgctgatt
      781 tgagtggggc aacaaaaggt cagcttgaag cctggcaaga gaatgccatg ttcgatacag
      841 ggcgttacag gcgtaaaaaa atccggtacc gcgatgaagt gactggaaaa atgataacgc
      901 gggataatcc accaatcccg ggaaagcaat cgctggcgaa ggggacgtca attcctctgg
      961 tcagtccggt tgagttttcg acatcatcgt ggcggcgggc tgttctgtct cttgaagagc
     1021 actataaagc ctggttgttg tggtgttaca gcgggagtat ttgttgggaa tatcagatcg
     1081 cgataacaca gtgggcgtgg aatgaattta atgctcaatc cggtaccaga aaaattgcag
     1141 ggaaaacgca ggaacgcctg aaaaaattaa tctggctggc ggcgcaggca gtaaaagcag
     1201 aactttttgg tgggaaaggt tatgaatacc aggagctggc attactggcg ggagtgacaa
     1261 ctaaaaactg gtccaaaaca tttactcgtc actgggttgc aatgaaacac atttttcacc
     1321 gactggatag tgaggcttta ttgtttgtaa tgagaacacg ttcaaaaaaa agacggcatt
     1381 ttcaaagcaa agtgttgcaa aagtagattg aaaggcatat attcatgcaa atctgatact
     1441 ttgccgattt tgtacgtgat ggcaaaagca aacaaaaccc gcccacaagc gggttttttg
     1501 tgccacttat ctcggataga catggtgaat gcgctggtgg aggagataag ggtgattttt
     1561 gaatgcttgc aacattgatt tcgtaacgtt attatcctgc
//
LOCUS       DAJTSI010000331         1597 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000331 DAJTSI010000000
VERSION     DAJTSI010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.339"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..636)
                     /locus_tag="OO327_004250"
     CDS             complement(<1..636)
                     /locus_tag="OO327_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR6020541.1"
                     /translation="MILSVFLGQPALTYAGVVIGSTRVVYLSNNADKSISVFSKEEKI
                     PYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEKILRIIHTKGVSLPDDRESVFWL
                     NIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAEN
                     PNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPANSAGGQMTWRFINDYGA"
     gene            complement(750..1322)
                     /locus_tag="OO327_004251"
     CDS             complement(750..1322)
                     /locus_tag="OO327_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HCR6020542.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          474 a          337 c          319 g          467 t
ORIGIN      
        1 tgcgccgtaa tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa
       61 aggtaacgtg agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac
      121 gatgacatcc atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc
      181 ttcccggcgc catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg
      241 tcgccagaac agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt
      301 gctcgctgat ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg
      361 caatgacaca cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg
      421 tgatactggc ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc
      481 tacccaagcc tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt
      541 atcagcatta ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt
      601 taatgctggt tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac
      661 tgttgtttta aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa
      721 gccaactgaa taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa
      781 tcagcgttcg ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac
      841 ttcatttcag ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta
      901 acctgcgaat tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct
      961 tgtcctgtac cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg
     1021 tcgccgtcgc cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt
     1081 gccgggcaat tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct
     1141 gaaaatgcat ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta
     1201 acttcacaag tagtgtcgac aatttcaccg acgaagttaa caacaccatc ttctgccagt
     1261 gcatgcccgg ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc
     1321 ataaaaactc cattgatata atatatcctt taccatgaat catcacttat attaataagt
     1381 gtgtttttga ttcaaaggaa tagtacacca aagagacggc tttatctaaa aataatatta
     1441 attacgcaaa aataaaatat agatttattg tttaaatatt atttaaacag taaaaaatca
     1501 agtttgttat cagacattaa tgataattca gaatatattt atatagaaag atagatgaat
     1561 ttattgatgt cagttataaa aaagggccac cggtcgg
//
LOCUS       DAJTSI010000332         1581 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000332 DAJTSI010000000
VERSION     DAJTSI010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.340"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="OO327_004252"
     CDS             complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="OO327_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HCR6020543.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLLKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGIMMGATLMLTWWRQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLK"
     gene            complement(1269..>1581)
                     /locus_tag="OO327_004253"
     CDS             complement(1269..>1581)
                     /locus_tag="OO327_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HCR6020544.1"
                     /translation="VAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWG
                     ATLKTIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQG
                     A"
BASE COUNT          482 a          400 c          406 g          293 t
ORIGIN      
        1 tttcaaaggg aggatgatga gatcgggaat aaaaacaaat acgactaata atgaatataa
       61 aaccaggacg taagggaaaa cgcctctgac tgcatcatcg aatttgagtt ttgccacccc
      121 tgaaataacg ttaagtacat taccgatagg cggtgtaatt aagccgattg agcagttaat
      181 gataaacatg acaccgaaat aaatcggatc gattcctgcc tctttaacta aaggcattaa
      241 taccggggta agaattaata ccgtcggcgt taaatccatg accatgccga caatcaggat
      301 agccaccata atgacgataa acagcagacg cggtgaatcg accaacggtt gcagtaaatc
      361 agaaaccatc atcggcagtt cagcaatggt aatcagccag gcggaaactt gtgccgaggc
      421 caccagaaac ataaccactg aggtggtttt cgccgcgcca atgagtacat gccaaagtgt
      481 ggcgaaggtc atttcacggt aaataactgt ggcgacaaac agcgcataga aagcagcaac
      541 ggccccggct tcggtagggg taaacagacc ggagcgaaac ccgccaataa tgatgactgg
      601 aagaaacagc gcccagatac cggagacaaa agagtgccag atttcctgca tcgttgcttt
      661 ttgctggcgg ggtaaattaa ggcgactggc ctgccgccac caggtaagca tcagcgttgc
      721 gcccatcatt atgccagggg caatgcccgc cataaacagc ttactgatgg ataatccgct
      781 ggaaacaccg aagataataa acggaatgga agggggaata attggcgcga taatgccgcc
      841 agaggcaatc agccccgccg cccggttgac cgggtagttg gcgctgcgca tcatcggcac
      901 cagtagagcg gcaacggcgg cggtatccgc aacggcagaa ccagaaaggc tcgccataat
      961 catagctgcc agcacgccga cgtagcccag cccgcctggt ttatgcccca ccagtttcat
     1021 cggcaggtca acaatgcgtt ttaacaagcc gcccgcattc atgatttcac ccgccagcac
     1081 aaagaacgga atcgccagga gggagaagct atcggctccg ttcaccagcg tttgtgccat
     1141 gatctggaca tcaaacatgt ccagccagaa cattaacgcc gccccgcaca acaacagtgc
     1201 ccaggcaata ggcaaaccga tagcaatacc acccaacaga cagcccagaa aaatcagcac
     1261 agccatgatt aagctccttg cggtggcgac gttagagaat tgctacgcgt gatgagttga
     1321 tataaatgac gcagttcaaa gaatgcgata acgaggctgg tgggcagaca agcggcatac
     1381 atcaggccga tgggcaaacc gaggatcggt gaataatcgc tccagtcctg aattgttttt
     1441 agcgttgcgc cccaggccag tgcgccacaa ataaataaga ttaaggaatg ggtaaccaga
     1501 gcgactcgtc gttgccatgc gggggagagt ttctccacca gaaaggtgac ctgaacgtgg
     1561 gcgttatcca taaaagccac a
//
LOCUS       DAJTSI010000333         1556 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000333 DAJTSI010000000
VERSION     DAJTSI010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.341"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            623..1222
                     /locus_tag="OO327_004254"
     CDS             623..1222
                     /locus_tag="OO327_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR6020545.1"
                     /translation="MQTYNTNPAYEMAPQLLEHFNQHLDSLFGVYSKLLPFRMDFAYR
                     KNTLSYRCACRYAMCAEMLQLINEVGEKLVGYAWVMEYTERKGLHIHFVGYLNGQSHR
                     SSYLVSRLMGDIWRRVTEGNGYYHWCRFNKNYPVNINHVIHYSDHKAVNDLRYAISYL
                     AKREQKECGIILKCSGLPEKSNRGRPRLDSPLPGICALV"
BASE COUNT          408 a          373 c          354 g          421 t
ORIGIN      
        1 ggcttcaccc gtgttaatat cctggataac tgtgtttgaa ggatttctgt taataatctg
       61 ctcctgcaac tcacctgatg agtattagtg gtaatacata atcttactcg taaaagagta
      121 taccttatgt cattaaacag tcttccggcg tgttgttcaa tcctgtacct ctgttactta
      181 tctggtacgt tgatatgtgt aatgctacgt taattgcaat atctggttat ggctttatac
      241 cttcagcatt taaaatattt tattaccgga gaaacaggga agtggaaaga ctttcgggat
      301 aatgtcaggc cattctgacg ttaatcacag taccaaataa cgagagggta atggaagtta
      361 aaacctgcgt aagttcctct gttgttcact ggaaaaagtg attgcccaca cgcgttataa
      421 aggcctttcc gctgcagcct ggttaacaga gcttcaggcg ttatatgcgc tgtatggctg
      481 ttacgtatgc tgttctttgc ctggttctga tacccgtgga agtcgttacc ggaggaaaca
      541 cgttcaccct actacctgag aacatcttct tcaaaaatta cggcaacgct tcttcctgtg
      601 ttacagaaca cgaggaagaa acatgcaaac ttataacacc aatcccgctt acgaaatggc
      661 tccccagctc ctggagcact ttaaccagca tctggattca ttattcgggg tctattcgaa
      721 actgttaccg ttcagaatgg attttgctta ccgaaaaaac accctcagct acagatgtgc
      781 ctgcaggtac gccatgtgcg cggagatgct gcaactgatt aacgaggtgg gagagaaact
      841 ggtcgggtat gcctgggtga tggaatatac agaacgtaaa gggcttcaca ttcattttgt
      901 gggctatctg aacggccaga gccacaggag ttcgtatctg gtatccagat tgatgggaga
      961 tatctggagg cgggttaccg agggtaatgg ttactaccac tggtgccggt ttaataaaaa
     1021 ttatccggta aacattaacc acgtcattca ctattcagat cacaaagcgg tgaatgattt
     1081 acgctatgcc atcagctacc tggccaaacg ggaacagaaa gaatgtggca tcatcctgaa
     1141 atgcagcggg cttcctgaaa aaagtaaccg gggtcgacca cgactggaca gccccctgcc
     1201 ggggatatgt gctctggtgt aaacccctga acgacaagta cgggctggcg ctaatctttg
     1261 ctgtcggttc cggcggccac ttcagcggca ctgcgcattt tttacggcgt ggtcattccg
     1321 tatgaaccat acggagattt aaccatgaca cattctttac cattccttcc ggcaaccgat
     1381 acagcacatc ccccaatctc catctctgct tactgactgt tttttacgtc agtgggccat
     1441 tgtgtgacaa ccacagtgct gtaacagcaa ccagcctgct ttacccaaac tccagtccag
     1501 attttaactg aaccgccatt cacaccctga ccacgctgcc cgtaccggga cggctg
//
LOCUS       DAJTSI010000334         1527 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000334 DAJTSI010000000
VERSION     DAJTSI010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.344"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..354
                     /locus_tag="OO327_004255"
     CDS             <1..354
                     /locus_tag="OO327_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR6020546.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
     gene            complement(392..1291)
                     /gene="nhaR"
                     /locus_tag="OO327_004256"
     CDS             complement(392..1291)
                     /gene="nhaR"
                     /locus_tag="OO327_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR6020547.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1357..>1527)
                     /locus_tag="OO327_004257"
     CDS             complement(1357..>1527)
                     /locus_tag="OO327_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR6020548.1"
                     /translation="ILCGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIG
                     YSWLRVRLRPSV"
BASE COUNT          442 a          367 c          338 g          380 t
ORIGIN      
        1 tatcagtttc atcatataag atttgactgc atccagggcg agcaaggtga attagttttt
       61 gaacccagtg acgttgaata ttactttgaa ccagtgacga tacaggagtc aggaaccagc
      121 atattaaaaa atgagttaga aaacagtgag gacggtgcgg gtcaaatagg ctttcaactc
      181 tccaatgatg gtacgaatga aatccagtat ggtaaaagca attactactc ttttcagcat
      241 ccccatgaag gaagtaatca gattcctcta tttattcgcc cgcgtacata tggaaacaat
      301 gtctcaagcg ggcaaataat gagccgagtc aaaatcgtgg taatgtacaa ctaatctatc
      361 tcccccacct taactttggg ggagctgccg attaacgcac cgctggacta aaaagcgcag
      421 aataatccgt attgcagatt cgctgtaccg ccgggtgctg aatcatccgc tcagcaaaaa
      481 tagcatggta ctcttccatc acattctcga cgcgaccaat ttctacgaca gttttatcgg
      541 cataaaagtc atatgcataa agcgttgggg caacgaagat tgcattgtgc atcgcaccaa
      601 aagctttcat caaagcggca tcatcaaact cgccgaggat ttctacgttt aatccctggg
      661 agttaaacca gttaagcaat ttgcgcccta acattgaacg tcgcccagga atcaaaagtc
      721 gccgttcttc cagacaagcc gggaacggtt tttctggtgg tggatttgta caccagaaac
      781 tcacgccaca ttcgccaatt ctcacggaga acaggccttc ctgctgcgta gagtctatcg
      841 gacagtcaga aatgatcata tccagtttat gctgacttaa ttgctccagc agcatttcgt
      901 gggtggattc gaagcagcga agatgaatgg gctcgccttc taccactgcg gcgttaagta
      961 cgctactgac caggcgtttg gaaagtgcat cagccacgcc aacgtcaaac aataaattgg
     1021 attctttgcg atagttcaca atatccagca tttcctggct taaggtgaac attttatcgg
     1081 catagcgata gaccagttct cccagctcgc tgggttcgag accacgtccc ttgcgtttaa
     1141 ataatttgcc ttgcaggcgc tcttccagcg ctcgaatctg tccggtaatg gtttgtggag
     1201 ttaaataaag cgcctccgct gcgccaacca cggaaccttc tttatagaca tgccagaagt
     1261 aatacaagtg gttgtaattg atatgagaca tgctcatttc tctccctgat aacaatgaaa
     1321 agggagccgt ttatggctcc ccggtaaacc gtcctgtcaa actgatggac gcaaacgaac
     1381 gcgtaaccag ctgtatccaa ttaccgccga agagatagaa ccgaccagga taccgagttt
     1441 cgcccagtta atcagttctg gatctacgct accaaaggcc aggctggcaa taaagataga
     1501 catagtaaaa ccgataccgc acaggat
//
LOCUS       DAJTSI010000335         1517 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000335 DAJTSI010000000
VERSION     DAJTSI010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.345"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            160..1422
                     /locus_tag="OO327_004258"
                     /pseudo
     CDS             160..1422
                     /locus_tag="OO327_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          394 a          398 c          369 g          356 t
ORIGIN      
        1 tttctgtttt catgcatcct tgcgaatctt tatctgatgt tgattcaaca ggttagtgtg
       61 aaaactcttc tcggtgcatt ttgattttac cctacgcatc gggaaaaatt ggtggtcaaa
      121 tctgggatca ggttagttcg ataatggagt gacccccata tatcccttac cgacgcaaaa
      181 atccgcaccc tcaagccttc tgataaaccc tttaaagtct ccgattctca cggtctgtat
      241 ctgctggtca agccgggtgg ctcccgccac tggtatctca aataccgtat tagcggtaaa
      301 gaatcccgca ttgcgctggg tgcctatcca gccatctccc tgtctgatgc gcgacagcaa
      361 cgtgaaggta tccgtaaaat gctggcgctg aatatcaacc cggtacagca gcgggctgct
      421 gaacgtggct cacgaacacc ggagaaagtt tttaaaaaca tggcgctggc gtgacataaa
      481 agtaacagga aatggtcgca gaacaccgcc gaccgtctgc ttgccagcct gaacaatcac
      541 atctttccgg tcatcgggaa cctacctgta tcagaactta aaccccgtca tttcattgac
      601 ctgctgaaag ggatcgagga aaaaggtctg ctggaggttg cgtcccgcac acggcagcac
      661 ctgagtaaca taatgcgcca tgcggtccat caggagttaa tcgatacgaa ccctgcagca
      721 aaccttggcg gcgtgaccac acctcctgtc agacggcact atcctgccct gccgctggag
      781 cggctgcctg aactgcttga acgtattggg gcatatcatc agggccgtga actgacccgg
      841 catgccgttc tgctgatgct gcatgtgttc attcgctcca gtgaactgcg tttcgcccgc
      901 tggtcagaga ttgatttcac aaaccgagtc tggacgatac ccgcgacgcg agaacccatt
      961 attggcgtgc gttattccgg ccgcggggca aaaatgcgaa tgccgcatat cgtccccctc
     1021 tcagaacagt ccatcgccat tctgaaacag attaaggata tcaccggtaa taatgaactg
     1081 atcttccccg gcgaccataa cccgtataag ccaatgtgtg aaaacacggt caataaggca
     1141 ctgcgggtga tgggttacga cacgaaaaag gatatctgcg gtcacggctt ccgggcaatg
     1201 gcatgcagtg cgctgatgga atcgggttta tgggcaaagg acgcagtaga acgccagatg
     1261 agtcatcagg agcgcaatac cgtgcgcatg gcttatattc ataaggcaga gcacctggaa
     1321 gcccgcaaag cgatgatgca gtggtggtcg gattatctgg aagcatgccg agaatcttat
     1381 gcaccgcctt atacaattgg taaaaataag tttatcccat agtatactaa cgaatcccac
     1441 actctggcaa attaccacca cctctacagg aggtggttct atatagactt ttatcaactt
     1501 catgacgaaa aacacct
//
LOCUS       DAJTSI010000336         1477 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000336 DAJTSI010000000
VERSION     DAJTSI010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.346"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..263)
                     /locus_tag="OO327_004259"
                     /pseudo
     CDS             complement(1..263)
                     /locus_tag="OO327_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(378..1016)
                     /gene="ospI"
                     /locus_tag="OO327_004260"
     CDS             complement(378..1016)
                     /gene="ospI"
                     /locus_tag="OO327_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCR6020549.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1175..1477)
                     /locus_tag="OO327_004261"
                     /pseudo
     CDS             complement(1175..1477)
                     /locus_tag="OO327_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          415 a          369 c          258 g          435 t
ORIGIN      
        1 agcaagcgct gtaactcttt tatttgtttt atagcggcag ccagttctga ggcaggaacg
       61 acatcctctc cggcagcaac agcagtgaga cttccttcca gatattattt tttccactta
      121 agcaacaggc tgggctgaat accatgcaaa cgcgccacat gggacaccgt catgccaggc
      181 tccattgtct gctgaataat ggcaattttt tcctgaggag ttttacgttt acgaagctcc
      241 agccccagca aagttccagt catctcaaaa ttagaactag tgttagacat atatccaagc
      301 ctatctctta ttttaagata catccactgt ctggcgtttc aaggggccgg ctcaaatcta
      361 tgtacaggct ctcagcatca gcaaagcctc ttacttttcc aactactact gttagcatag
      421 tgatcaaatt cctgatcaga tacatgaatt acctgaaccg aactattttc atctacaaat
      481 gcatctggcg atttttctac aggatcataa agattaaatc cctttcctgt ttgaacatcc
      541 aaaaacataa ttttttcacc atcgttaaat ccagctatcc aatgagtggc ctgttcaata
      601 gaaatcatta ctacacttcc ttttgaagta ttttgtaaaa tattttctgc aatacgctca
      661 cgaaatttac tataagtttc attaggatgt gacactatgc cttcactatc aatttgctct
      721 aaatgcctat gaaaaaatcc ggtcatatac tctgatattt cattaggtac gggagcctct
      781 cttactccat accctaacat agccatacaa gcatgaagcg cacaccctga acagttacca
      841 tccccaccag acggatttat cattttctgt aaagtcgctg ccaattcaga atctttatcc
      901 atatcaccaa gacttttaga gtttaaagat tccagcaatt ttttaacatt tacatttccc
      961 tcctctgtat gtgcctcata tccagcagtt ccctgtaacg acaccccatt aatcatattg
     1021 tatcctgaca gtatatatac tcactatatt ttcttcataa caaatatccc tctgatatga
     1081 caacaaaaaa cggagccgga ttcctgtttt tgtgaactgc agctattcgt gtctaaaaag
     1141 tgatccactt accaccaacg gcctaatata tacagaacgg agtaaacagg actaacagtt
     1201 cccgacaggt ctcatcattc cgtggcccga aagtgtaagc caacacaccg ccagttttag
     1261 tgttgtacga ataccagagc cagtgttgtt gcagccttgc tgccaacgaa gcgccactgc
     1321 tcatcaagtt cacagataag ggcaacatca gcgtgagcga ctggcgcaga tgttatccgc
     1381 cggggcggga gctttttaat gtccgtatga cggtattgat gccgattttc agtgtcctgg
     1441 cggtatcacg gccccctgcg ccgttatgtg ccatttc
//
LOCUS       DAJTSI010000337         1420 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000337 DAJTSI010000000
VERSION     DAJTSI010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.347"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..327)
                     /locus_tag="OO327_004262"
     CDS             complement(<1..327)
                     /locus_tag="OO327_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HCR6020550.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLS"
     gene            complement(336..605)
                     /gene="fliQ"
                     /locus_tag="OO327_004263"
     CDS             complement(336..605)
                     /gene="fliQ"
                     /locus_tag="OO327_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR6020551.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            complement(615..1352)
                     /gene="fliP"
                     /locus_tag="OO327_004264"
     CDS             complement(615..1352)
                     /gene="fliP"
                     /locus_tag="OO327_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCR6020552.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            complement(1352..>1420)
                     /locus_tag="OO327_004265"
     CDS             complement(1352..>1420)
                     /locus_tag="OO327_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="with FlhA, FlhB, FliP, FliQ and FliR is one of the
                     membrane components of the flagellar export apparatus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliO"
                     /protein_id="HCR6020553.1"
                     /translation="EEIPQTDFQSVMKNLLKRGGRS"
BASE COUNT          389 a          381 c          369 g          281 t
ORIGIN      
        1 tgacagcccc atttgcagac cgataatttc gccagcggtt cgcacagcgg caaaggcaaa
       61 ttgcatggta aaaccaagcg caatgccgat caggatctgc tgtacggcca gccacagagc
      121 aaagaacgaa aaaacaggaa catcgttggc aggtaatgat ggggcaatgg cgaacgtgat
      181 catcattgcc agacccagtt ttacccgttt cggcacgctg cgttcgctca gaattggcgc
      241 ggtagaaatg agcgccagca cgcgcagtaa cggccagaag tacaggctta accaggaaag
      301 ccattgatcg cttgtctcct gcatcattgt acgggctacc cgatgatata cggcaggtta
      361 gtgaacaagg tgcggacgta atccagcaac agattgagca tccacggtcc ggcaataatg
      421 atggcgataa atacggcgat gattttcgga ataaacgaca gcgtcatttc gttaatctgc
      481 gtggctgcct gcaaaatact gatgataagg cccgtgacca gcgctaccag caacagcggg
      541 gctgccagtg ccagcgcgac tttcatcgct tcagttccca tcatcatgac cgattcaggt
      601 gtcattttgc ctctctagct gtaaaagctc tgcgccagcg aaccgaccag caattgccag
      661 ccatccacca gtacaaacag catcagttta aagggcagag caatggtggc ggggggaacc
      721 atcatcatcc ccaatgccat caacacgctg gcaatcacca ggtcgataat caaaaaaggg
      781 atgaaaatcg tgaagcctat ctgaaatgcg gttttcaact cgctggtcac gtaggccggg
      841 agcaaaatgc gcatcggcac agcttcaggc ccctgcaacg ggccggtatt cgccagtctg
      901 gcaaacaacc ctaaatctgc ctcgcgggtc tgacgcagca taaactcacg cagcggctgc
      961 gccccttttt ccagcgcctc ctgcattgag attttctctt cgctgaatgg ctggtacgca
     1021 tcgacataaa ttttgtcgat cactggcgac ataataaaaa aggtcaaaaa cagtgccagc
     1081 cccagcaata cctggttagg tggcgcggag ggcgttccca gcgcgttacg caataaacca
     1141 aaaacaataa tgatgcgggt gaaactggtc atcatcagta aaatcgctgg aatgaacgtc
     1201 aacgaggtga tgaacaccag cgtctgcacc gggagcgacc agctttgtcc accgccaggt
     1261 agcggctggc tggtgatacc cggcagttgc gcgaaggcga ggggcgtaat cagccagaga
     1321 aggaccggtg cgacagaaaa caaacgacgc atcaggatct cccgccacgc ttaagcaaat
     1381 ttttcatgac cgactgaaaa tcggtctgcg gtatctcttc
//
LOCUS       DAJTSI010000338         1413 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000338 DAJTSI010000000
VERSION     DAJTSI010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.348"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..181)
                     /locus_tag="OO327_004266"
     CDS             complement(<1..181)
                     /locus_tag="OO327_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6020554.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKA"
     gene            complement(246..1196)
                     /gene="virK"
                     /locus_tag="OO327_004267"
     CDS             complement(246..1196)
                     /gene="virK"
                     /locus_tag="OO327_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR6020555.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSGYRKRYALLDYIHETTIRSLDAYPVHSEHQNLN"
     gene            complement(1201..>1413)
                     /locus_tag="OO327_004268"
     CDS             complement(1201..>1413)
                     /locus_tag="OO327_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR6020556.1"
                     /translation="IGGIPEVIQNNQTGTLLPASNKHAWMCALNDFFNDPGRFYQMAR
                     LAKQDIEERFDINKTALKILTLAKQK"
BASE COUNT          434 a          304 c          265 g          410 t
ORIGIN      
        1 gagctttttt gctcaatctg cccgcccaac gccctatttt tgccaagaca ggatctctga
       61 atgaaggggg aaacattgaa atacctgcaa tcattcccaa aaggaaccat gtagaccagt
      121 aaacagggga aaggtaattt ttcttaaatt caggaataaa ctcagattta tattttttca
      181 ttatttagtg cgaatgcctc taccaaaatt atcattactg ttttattcag agaatacatc
      241 aaaccttaat ttaaattctg atgttctgag tgtacaggat atgcatccaa agagcggata
      301 gtagtttcat ggatataatc cagcaaagcg tatcgttttc ggtacccaga gcgtttttta
      361 ctggctatat cgcttagcgg ctttcgaatc acctgggttg gtaatctgta ccagtcacca
      421 caggtttttc ctgctacaga ctcccagaaa tcactataga ccgcaacaaa ggttttgcgt
      481 ttctgatacc agtatctcaa ttgccggaaa acatgactgt gctcactgac tgccagacac
      541 tcagagatat tgcaagcttt catcaacgca caaaatgctt caaaaataat ccgttttggg
      601 aaaatcccat aacaggcccg ggttgcgcac cgaataacat caggtccggt atttttaggg
      661 gctccctgta atccgccaat aaaagcaatg tgtccatttt gagtcagaat gactgaaaat
      721 gttaaccggg caataactgt tttattgtaa aacaatgaca acatcaactc tccttcccgg
      781 tcaaaaccac tgggagaaca ctggatatca aaattctctc catctttccc gatgaattgg
      841 actaatgata ccgatttttg tggaaacaaa aatttagaat atttttctgg caaattttgc
      901 acaaagcgat aatgctcaag aatatattgt ccacgggtcc aggcgcgccc ccctttatgc
      961 aaataagggc gatgaattct tgctggcaac gtggggtgta ttgccagaat atcctcaatg
     1021 cacggcaagg aacgcagttc ataataatat cgaatactga caaccggatt tatagaacat
     1081 cgcaaaatgt acatgaaccg aaattttctg tgttcccact tccccggtag tgaatctgag
     1141 gaaaaaataa tccttttaag gaaaccgata aatgataagt tacttataga aaacatattg
     1201 ttacttttgc ttcgctaatg tgagtatttt taacgcagtt ttattaatat caaaccgctc
     1261 ttctatatcc tgttttgcca ggcgagccat ctgataaaaa cgcccagggt cattaaaaaa
     1321 atcattcagg gcgcacatcc atgcgtgctt attacttgct ggtaacaatg tcccggtctg
     1381 gttgttctga ataacctcag ggattccacc aat
//
LOCUS       DAJTSI010000339         1411 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000339 DAJTSI010000000
VERSION     DAJTSI010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.349"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            31..291
                     /locus_tag="OO327_004269"
     CDS             31..291
                     /locus_tag="OO327_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HCR6020557.1"
                     /translation="MAWARHYQQIVREEKETELADDLEKGLPQHLFESLCIDHLQRHG
                     ANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
     gene            297..1394
                     /locus_tag="OO327_004270"
     CDS             297..1394
                     /locus_tag="OO327_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6020558.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAG
                     YEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSIRIQMEKQMNIETGQVWRTARILKD
                     RSTRIDGQVFSNPTFKNFLPHIESLNLGGEHPGIDTSRDNSELFANDGTPTWLKEKRA
                     KEIALDEIIELLNKHHGGTSNDAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNAL
                     WIELEGVEYAFPDPQTQNETQQAGYDENIPV"
BASE COUNT          437 a          319 c          346 g          309 t
ORIGIN      
        1 gcttatccga tttttgatcg tattgaagaa atggcatggg ctcgtcacta ccagcagatc
       61 gttcgtgaag aaaaagaaac ggaactggcg gacgacctgg aaaaaggtct gcctcagcac
      121 ctgtttgaat cgctctgcat cgaccatttg caacgccacg gtgccaataa acaggcaatc
      181 agccacgcat ttgatgatga tgttgagttt caggagcgca tggcagaaca catccggtac
      241 atggctgaaa ccattgccca ccaccaggtt gatattgatt tggaggacta aaatctatgg
      301 cgctttttca acgagcaata aacacacagg cctatctaaa agcaggaatt atggggtttg
      361 ctggcgacgg caaaacctac acagcaagcg aactggctat tggtctggta atgcttatgc
      421 ggcaacgggg aattgaagct ggtaaccgcc cggtaatgtt tctggacaca gaaacaggct
      481 ctgactgggt taaaccccgc tttgatgcgg aaaatatcga attatttacg gcaaaaaccc
      541 gctcgtttgt tgatctgctt gaagccatta atgaagcgga aagcagcggt tccgtaatga
      601 tcatcgactc catcagtcac ttctggaccg gattatgcga cgagtatgcc agacgccgaa
      661 accgtaaacg aggactggaa ttttcagact gggcgtggct caaacaggag tggcgacgct
      721 ttaccgatcg atttgttaac agtcaggcgc acattattat gtgcgggcgt gcaggatatg
      781 agtatgactt tttcgaaggt gatgatggca aacgccagtt ggcaaaaacc ggtataaaaa
      841 tgaaagcaga aacagaaacc ggttatgaac cctccatccg gatccagatg gagaagcaaa
      901 tgaacataga aaccggtcag gtgtggcgca ccgccagaat actaaaagat cgctccaccc
      961 gtattgatgg acaggtattt tcaaacccaa catttaaaaa ctttcttcct catatcgagt
     1021 ctcttaacct gggaggtgaa caccccggca tcgatacctc acgggataac agtgaattat
     1081 ttgccaacga cggtacaccg acatggctaa aagaaaagcg tgcaaaagaa atcgcacttg
     1141 atgaaattat cgagttgctc aacaagcacc atgggggcac cagtaacgac gccaaacgcg
     1201 caaaagctga tttgctggaa aaaacattca cctcccgctc atgggaaaga atcaaaggta
     1261 tggactggcc gacaatcaaa gctggtcgta atgcactatg gattgaactg gaaggcgttg
     1321 aatatgcatt cccggatcca caaacacaaa acgaaacgca acaagcagga tacgatgaga
     1381 atattccggt gtaaaaccaa gtgcattttg g
//
LOCUS       DAJTSI010000340         1404 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000340 DAJTSI010000000
VERSION     DAJTSI010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.350"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..167
                     /locus_tag="OO327_004271"
     CDS             <1..167
                     /locus_tag="OO327_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HCR6020559.1"
                     /translation="AIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQRM
                     KERRKEWGTL"
     gene            152..790
                     /locus_tag="OO327_004272"
     CDS             152..790
                     /locus_tag="OO327_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCR6020560.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1018..>1404
                     /locus_tag="OO327_004273"
     CDS             1018..>1404
                     /locus_tag="OO327_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020561.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSS"
BASE COUNT          407 a          257 c          347 g          393 t
ORIGIN      
        1 ccgctattgc cagtacacct cacccgtcct ggcgacgtat ctgtaagaca ctcataaaga
       61 atgatttctg gtgccggact ctctccttca gtccgaacaa accccggcac tatgaacgtt
      121 acctgcagcg tatgaaggaa aggaggaagg aatgggggac tctgtgacac cggagatact
      181 tggcaacatg attcgtcagt atttcagtca ggtacgaact gaaaaagaga gcatacaggc
      241 tctgaatcat ctgcgcagag tgcttcatga ggtcagtcct tttgcacagg agcctgtgga
      301 ctgtgtgttg tgggtgaaag cggatgaggt ggtggccaac gattacaacc ctaatgtcat
      361 ggcactaggt gagaaaaaac tactgaaaca gtctctggag aaagatggtt tcacacaacc
      421 ggtggtggtg tcagaggaga agaatcatta tctggtggtg gatggttttc atcgtcagtt
      481 actggggcgg gaagcagatg caggaaaacg cctgaggggc tggttgcctg tagcctgcat
      541 taatccggag agaaagggac aggccgcacg tattgccgcg accattcgac ataaccgggc
      601 cagggggaaa caccagatca ctttaatgtc tgatattgtc agggatcttt cacgtctggg
      661 ctggacgaat gaacgtattg gtacagagct gggaatggac caggatgaag tgctgaggct
      721 gaaacagatt agcgggctga cagagttgtt tcaggaagag gatttcagct cggcttggac
      781 ggttcgttga tgttgttaaa tatgggcggt atttctgaaa gttgatactt tgggtagaaa
      841 aaatgatacc gaaaactatg gaggatgtgc ttgtcaggtg actggacacc ataggtctta
      901 ggctttgata acgggataag atgagcggct tttctcatac catcaggtgt gatgatggcg
      961 cagatttaca aaatctgatt gattctaaag atgtataagt aaaaatcaag ctttgatatg
     1021 aaaaaacaga tatttattaa taataaacct cctgtagtcc cttactcagg gacgcacgcg
     1081 aaaatattta aatacattga aatccctctg ccgtttttct actttatcta tacatcaggc
     1141 gaaccattcc acatatctgt ccaaaatact gttatttatg tatcaaaata caatggtata
     1201 tttattaata aattagttcc tttttctctt ttatttgaca gggatatatc tgttcttcaa
     1261 agaagagata tttgtgttgt gcgatttacg tcagaggaaa taagtgaaca taacgtttta
     1321 tttgatcatg acattgaacg attaaaaaaa atttccaaag ctcagcttat atctcccgat
     1381 tatgttttga ttgatttttc tagt
//
LOCUS       DAJTSI010000341         1367 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000341 DAJTSI010000000
VERSION     DAJTSI010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.351"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(143..1093)
                     /gene="virK"
                     /locus_tag="OO327_004274"
     CDS             complement(143..1093)
                     /gene="virK"
                     /locus_tag="OO327_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR6020562.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            complement(1098..>1367)
                     /locus_tag="OO327_004275"
     CDS             complement(1098..>1367)
                     /locus_tag="OO327_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR6020563.1"
                     /translation="FGMVLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAW
                     MCALNDFFNDPGRFYQMARQAKQDIEERFDINKTALKILTLAKHK"
BASE COUNT          421 a          303 c          256 g          387 t
ORIGIN      
        1 gaaccatgta aaccagtaaa caggggaaag ataatttttc ttaaattcag gaataaactc
       61 ggatttgtat tttttcatta tttagtgcga atgcccctac caaaatcatc attaatattt
      121 tattcagaga atacatcaaa ccttaattta agtcctgatg ttctgagttt acaggatatg
      181 catccaaaga gcggatagca gtttcatgga tataatccag caaagcgtat cgttttcgat
      241 actcagagcg ttttttacta gctatattgc ttagcggctt tcgaaccacc tgtgttggta
      301 atttgtacca gtcaccacag gttttccctg ctacagactc ccagaaatca ctgtagacag
      361 caacaaaggt tttgcgtttc tgataccagt atctcaattg ccggaaaaca tgactgtgct
      421 cactgactgc cagacactca gagacattac aagctttcat caacgcacaa aatgcttcaa
      481 aaataatccg ttttgggaaa agcccataac aggcccgggt tgcgcaccga ataatatcag
      541 gtccggtatt tttgggggct ccctgtaatc cgccaataaa agcggtgtgt ccattttgag
      601 tcagaatgac tgaaaatgtt aaccgggcaa tcactatttt attgaaaaac aatgacaaca
      661 tcaactctcc ttcccggtca aaaccactgg gagaacactg gatatcaaaa tcctctccat
      721 ctttcccgat gaattggact aatgataccg atttttgtgg aaacaaaaat tcagaatatt
      781 tttctggcaa attttgcaca aagcgataat gctcaagaat atattgcccc cgggaccagg
      841 ctcgcccccc tttatgcaaa taaggtcgat gaattcttgc tggcaacgtg ggttgtattg
      901 ccagaatatc ctcaatgcac tgcaaggaac gcagttcgta ataatatcga atactggcaa
      961 ccggatttat agcacatcgc aaaatgtaca tgaaccggaa ttttctgtgt tcccacttcc
     1021 ccgggagtga atctgaggaa aaaacaatcc ttttaaggaa accgataaat gataagttac
     1081 ttacagaaaa catattgtta cttgtgcttc gctaatgtga gtatttttaa cgcagtttta
     1141 ttaatatcaa accgctcttc tatatcctgt tttgcctggc gagccatctg ataaaaacgc
     1201 ccagggtcat taaaaaaatc attcagggcg cacatccatg cgtgcttatt acctgctggt
     1261 aacaatgtcc cggtctggtt attctgaata acatcaggga ttccaccaat ctgactggcc
     1321 agtacaggca cagaaaatgc cgatgcttct gccagtacca taccaaa
//
LOCUS       DAJTSI010000342         1360 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000342 DAJTSI010000000
VERSION     DAJTSI010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.352"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(40..115)
                     /locus_tag="OO327_004276"
     tRNA            complement(40..115)
                     /locus_tag="OO327_004276"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(80..82),aa:Ile,seq:cat)
     gene            complement(292..981)
                     /locus_tag="OO327_004277"
     CDS             complement(292..981)
                     /locus_tag="OO327_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR6020564.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQKA
                     AFSKQSIAKVD"
     gene            complement(978..1337)
                     /locus_tag="OO327_004278"
     CDS             complement(978..1337)
                     /locus_tag="OO327_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6020565.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDETNIVRGL
                     PVPGGRLGIKITELECA"
BASE COUNT          331 a          375 c          286 g          368 t
ORIGIN      
        1 tagaaggttg atgctctatc cggatgagct agtggtggtt ggtggccctt gctggacttg
       61 aaccagcgac ctggcgatta tgagtcgctc gctctcacca ctgagctaaa gggccggagg
      121 cagaataata accatatgtc atcacatctg caaactcatc tgaccaccag cacgtttaac
      181 gtcctgtgcc atttttcagg cataaaaaaa ccgctcggcg gcgggttttg cttactttac
      241 catcgcgtac aaaatcggca aagtatcaga tttgcatgag atatatgcct tttaatctac
      301 ttttgcaata ctttgctttg aaaatgccgc tttttgttct gaacgtgttc tcatcacaaa
      361 caataaagcc tcactatcca gtcggtgaaa aatgtgtttc attacaaccc agtgaccagt
      421 aaatgttttg gaccagtttt tggttgttac tcccgccagt aatgccagct cctgatattc
      481 ataaccttcc ccaccaaaaa gttctgcttt taccgcctgc gccgccagcc agattaattt
      541 tttcaggcgt tcctgtgttt tctctgcaat tttcctagtg ccagaatgag ccttaaactc
      601 ttcccacgcc cagtgggtta tggtgatctg atactcccag caaatacttc cgctgtaaca
      661 ccacaacaac caggctttat agtgctcttc aagagacaga acagcccgcc gccacgatga
      721 tgtcgaaaac gcaacctgac tgaccagagg aattgacgcc cccttcgcca gtgattgttt
      781 tcccgggatt ggtggattat cccgcgttat catttttccg gtcacctcat cgcggtaccg
      841 gattttttta cgcctgtaac gccctgtatc gaacatggca ttctcctgcc aggcttcaag
      901 ctgacctttt gtcgccccac tcaaatcagc ggtggcgata atgagctgct cacgaacaaa
      961 ctgtaaatac tggttattca tgcacactcc agctctgtga tttttatccc caaccgacca
     1021 ccaggaacag gcagtccgcg cacaatattg gtttcatcaa actgctcgtc gtcgataagc
     1081 aaccccgcat gtgtcagtgc atccagtggt gctttcagga tattgtccag gtcacgacgc
     1141 cgcttatccg gtggctctgc aataatcttt atcgccagtc ttccggacag gcttaatttc
     1201 agtcgctgct gacggacaat aagcgccaca tcccggcgat aacgctcacc ggcttttgat
     1261 acaaaatatg tgctgccacg acgtcgccag taagtattca ccgtcggcgg ataaggtaaa
     1321 ataaactcat ggcgcatcag cgcagcacct cctgcaccag
//
LOCUS       DAJTSI010000343         1356 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000343 DAJTSI010000000
VERSION     DAJTSI010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.353"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            141..470
                     /gene="bsmA"
                     /locus_tag="OO327_004279"
     CDS             141..470
                     /gene="bsmA"
                     /locus_tag="OO327_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR6020566.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            619..894
                     /gene="yjfN"
                     /locus_tag="OO327_004280"
     CDS             619..894
                     /gene="yjfN"
                     /locus_tag="OO327_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR6020567.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(1011..>1356)
                     /locus_tag="OO327_004281"
     CDS             complement(1011..>1356)
                     /locus_tag="OO327_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR6020568.1"
                     /translation="GNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRLQ
                     QQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQN
                     DLLLRATGGVCV"
BASE COUNT          363 a          348 c          321 g          324 t
ORIGIN      
        1 agcacaagtt tctgctggcg gtattagttc ataatgttga gatgtgggtt acgctttcgt
       61 cacgttttca ttcgaaaatc gcgtaccagg taacaattcg ggaacattcc cccaaagtta
      121 aattaataag gcactacact atggttagca ggaaacgtaa tagcgttatt taccggtttg
      181 ccagtttatt attggtgttg atgttaagtg cctgtagcgc actgcaaggt acgccacagc
      241 cagcaccgcc agtaacggac catccgcaag agattcgccg cgaccagacg caagggttac
      301 agcgaatagg tagcgtaagc acgatggttc ggggttctcc ggatgacgca ttagcagaaa
      361 tcagagcaaa agctgtcgct gcaaaagctg attattacgt tgtcgtcatg gtggacgaaa
      421 ccatcgtgac aggacagtgg tattcacaag ccattttgta ccgtaaataa atcagacaac
      481 ttacatagtc agctttacat tgctttgcgt ccctatgcgt ttacctgtgc acaataaatt
      541 accggctgga agcttgggcg tttattaccg cgatggaatg ccctgcaaca tgtggggaaa
      601 cgaaaaatgg agctgacgat gaaacaatta cttgcctcac cctcgctgca attagtgact
      661 tatcctgcga gcgccacggc acagtctgca gaattcgcca gtgctgactg cgtgacgggc
      721 ctgaatgaaa taggtcagat ctcagttagc aatatttcag gggatccgca ggacgtggaa
      781 cgtatcgtgg cgttaaaggc cgatgaacaa ggtgcgtcat ggtaccgcat tattaccatg
      841 tacgaagacc agcagcctga taactggcgc gtacaggcga tactgtatgc atgagtgtca
      901 tctacagcaa atgaacaatg ccggatgcgg cgcgagcgcc ttgtccggcc tacaattttc
      961 acatatttca tttagttaat cgaaaccagc gtcgcatcag tcgtatacgc ttacacacac
     1021 actccccccg tcgcccgcag caataaatca ttctggatct gctctgacaa ccgtacgccg
     1081 ccgcgcgtat ctaacatcac ctgacaccac gcctgcgcca ttggcggaga agcatatttc
     1141 aacatttgcg caccgcagcc cagcaggaat agctgatgag taatctctcg ccccagttct
     1201 tcagctggct tacgcagctg ctgctgtaaa cgacgaaccg cgcgatcaaa atagcgatcc
     1261 tgccctttca cttccacaaa tgcttccgac aataagtcgt atacgcccgc ttgcttattg
     1321 agaacgcgca acacatccag gcacataata ttgccg
//
LOCUS       DAJTSI010000344         1353 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000344 DAJTSI010000000
VERSION     DAJTSI010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.354"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..482)
                     /locus_tag="OO327_004282"
     CDS             complement(<1..482)
                     /locus_tag="OO327_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6020569.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(473..775)
                     /locus_tag="OO327_004283"
     CDS             complement(473..775)
                     /locus_tag="OO327_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6020570.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1047..1319
                     /locus_tag="OO327_004284"
     CDS             1047..1319
                     /locus_tag="OO327_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020571.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          398 a          341 c          287 g          327 t
ORIGIN      
        1 cagactttat aaaacaaggt attaggtgcc acaactgact gaacaaaacc aaaacgctca
       61 taaaatgctc tggcgtcatc atcaattgca tgtaccataa cagccttaat gccaacctga
      121 tcagccagat tgacaacccg gtgaatggca tcacttagta gccatttacc gaaaccatgc
      181 ccctgggtac aaacatcaac agccagacgc cctaacagaa caacagggac aggattgggc
      241 atgttatgcc gcaaactgcg accaagctcg gcatgagtga cggaaccagt agctaatgag
      301 tagtatccca caatacgtaa agtatcctcc aagcaaataa cgaacgtgcg agaagcattg
      361 agcatctggt ttttcatagc ccgaccacgt aaccagttac ttagcacctc atttccacag
      421 tcaaatggct gaagtatatg ctcatccgtc aacaatgctg gcgcggtgac atttatttcc
      481 attcaggctt taccgccatc agacgttcac gcccttctgc attctgcaca ggagcttcca
      541 gttgcgtaat aaacgaatca taacgttcgt tatccagcac aaataaacgt tgatccagga
      601 taatgttttt agcttcctgg tacgccagtt ccatgatgaa atcggtacgg ttcttattga
      661 ccaagctcgc agcataatca ataacggctc gttcctcatc agttgcccgg atgttaatct
      721 gatgttctcg cggtgtttta cggacgcttg cagctgtaga cataaaaacc tccattggta
      781 acaatttgct atgcaattaa tgttatcgca aatggtaaca gaacgctata cagaatcact
      841 tacgggaaaa gcgatcccct gataaaaaca atggcagaga cctgaacatt gtgacgatga
      901 tctgtgtcgg cgcacaaatc atgtctgatt acggccgttt aatgtgattt tgataatgaa
      961 atgcctgaac cacggcagcc tggcggctgc aaatcggggt gtggcagaat atgagctttc
     1021 agagtaacca tccagaatga gttcccatgt cacacaacct ggagcaccag aaagtccaca
     1081 ccagaatggt gaaagaggtt ctgaaagccg tcgccagagc caataatcac ccttataagt
     1141 ccgtatttgc agactttatc accggtcatc cctcctgcac ggtctgtttc tgggaaacct
     1201 tccacaaaat gtacccagat tcaccgtacg aatacgtcac gttctgccat acctgccgtc
     1261 gttttgattt atacgaaaca gaagcggaga tgaaggcgga tgacccgaag tggtggtgat
     1321 tttcagcccc gaccgctgaa acgcctgttc aca
//
LOCUS       DAJTSI010000345         1344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.355, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000345 DAJTSI010000000
VERSION     DAJTSI010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.355"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            378..1127
                     /locus_tag="OO327_004285"
     CDS             378..1127
                     /locus_tag="OO327_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR6020572.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
BASE COUNT          381 a          271 c          271 g          421 t
ORIGIN      
        1 aaatattggc tccttctgat gccattctat ttcaggaagg agtgtccgtt aaactcaggc
       61 tacctcaaaa agttatcggt gcgtttatcg aaaaacatat gttctactaa ttggagtcat
      121 tgtctgtaaa agcaactcaa aaatacaagt tgcttattcc ccttgttggt aagaacttaa
      181 ttctaactgt ttgttctggg tgttttaaac gtcctccttt ttatgcttgt tcataacgga
      241 tatttaatag gtaacctttg ttttagcatc ttctgattta tttaaattat gtgatagtac
      301 acacaatttt attatccagt actttatgct cttaataaaa taattataaa tatgtatcct
      361 ctataaggag atgttatatg aaaagacagc tttttactct tagtattgtc ggggtatttt
      421 ctttaaatac ctttgcatca ttccctccgg gaaatgatgt gacaacaaaa cctgaccttt
      481 actacctgac aaacgataat gctattgaca gtctggcatt attaccgcct ccaccacaaa
      541 tcggaagtat tgccttcctg aatgaccagg ccatgtatga aaaggggcgt ttattgcgaa
      601 atactgaaag agggaagctg gcagctgagg atgccaatct cagtagtggt ggcgtggcaa
      661 atgtattttc tgctgctttt ggttctccga taacggctaa agattcacca gaattgcata
      721 aattactgac aaatatgatt gaggatgccg gagacctggc aacgcgttca gcaaaagaat
      781 attatatgcg tattcgacct tttgcttttt atggtgtttc tacgtgtaat acaaaagaac
      841 aggatacatt atccagaaat ggctcttatc catcaggtca tacatcgatt ggttgggcaa
      901 cagcacttgt attgtccgaa ataaatccgg cacgccagga taccattctg aaacggggtt
      961 atgaactggg ggacagcagg gttatttgcg gttatcactg gcaaagtgat gttgacgcag
     1021 cacgtattgt tggctctgct attgtagcaa cactgcactc aaatcctgtg ttccaggcac
     1081 aattacaaaa agcgaaagat gaattcgcta acaatcagaa aaaataatgc ttttagccag
     1141 tacgccttat aaccccgacc ttcagggcta atgccagtca gttaagcaac tgactggctc
     1201 tttttcaggg ctgtggggta tttccagtgc ctctccttta ccactctcgg aaaggccctt
     1261 tcccttcttg tcggtaattt cactaggctg tgtcccttaa ttacgtaagc gttgataaaa
     1321 gagtcggatg cagcccagtt tcac
//
LOCUS       DAJTSI010000346         1340 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.356, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000346 DAJTSI010000000
VERSION     DAJTSI010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.356"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..872)
                     /gene="ipaH4.5"
                     /locus_tag="OO327_004286"
     CDS             complement(<1..872)
                     /gene="ipaH4.5"
                     /locus_tag="OO327_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCR6020573.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GPQIYFSMSDGQQNT"
BASE COUNT          373 a          243 c          301 g          423 t
ORIGIN      
        1 gtattctgtt gtccgtcact catggagaag taaatctgcg ggccgtggta gtccggcgaa
       61 gaggttaatc tttgcaggga ttgcagaacg tgagtcgaca atggattccc tgaaatgtta
      121 acattcgtac aaatgggcaa tgtgattata ttttcgggta atgaagttag taggttgttg
      181 gaaacatcca gtgccaatag ttcttgcggt aatctggata atctgcgtag ttgattttca
      241 cctaccttca aatattttaa gctctgagga aattctggta gttcctgtag tctgttgccg
      301 gaaacaaaga gttcttgtag tctatgaggc aattcaggca agacagtaag gcgattacct
      361 tcaatacgta atattgttag cgttgatgga agctctggta acgtctccag actattaaaa
      421 cgtgccgaga gtgattgtaa atatggtggt aatgatggca gacatgacaa atttcgattg
      481 aatgaaatat taagcttaat cagttgagat ggaagtgtcg ggagtatatt caagttgtta
      541 ccatttacat gaagttctgt caaaagcgga gaattttcag gtagggagat taactcattg
      601 ttggaaatat taagttcacg tatatgcaaa gggagtggtg gtaaagaacg tagtttcagt
      661 aagcttaaat tgagaacagg ctcccgggta tccagacata ttttcaataa tcgaaccgcc
      721 tggatgcgat tttctgttgt tgttccttcc ctagcccagt catcccagat gcggtggtaa
      781 tctctggtac actgttcttc taccgataaa cgagatatac atgataagcc acaaagggaa
      841 cgaaaaaaag aatgattgtt gatcggtttc atttattaac ccctcactat tcatatcttg
      901 gtctattaat gcaaataaaa cgagaaagtc tttaaaaaaa caatcctaga gctttatcgg
      961 cgttttcatt ccggctggag gaatatgttc ctgtctttcc agtaaccgtc gattgttata
     1021 tcagtctacc catgttaatg tgaccagttc tactttcatc cagtttggat tgatctttat
     1081 cccctatatt ttcaaacaat ttttatcatt taactcatta ctgtctcgat attcccatag
     1141 caaacgatct ggttggttat cccctcaaaa aagtcagttg cacgccgtaa atccctgccg
     1201 gtattgagcg aggatttacg ggttgttgtt aaggtccgta atcgggtcac agtttttccg
     1261 gagtcaatcc ggtctgcggt ttatgcttat gcgacgtgat tatgaatggt gcagtcgtga
     1321 gccgttttca gacaatcgca
//
LOCUS       DAJTSI010000347         1317 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000347 DAJTSI010000000
VERSION     DAJTSI010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.357"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>1317
                     /locus_tag="OO327_004287"
     CDS             <1..>1317
                     /locus_tag="OO327_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6020574.1"
                     /translation="QMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTV
                     DADPQAEISSKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQ
                     KSLIALANKAVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPG
                     FQLINQRITHSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQ
                     ENQIAQFTAADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLA
                     AGKPLTRATDNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLR
                     VNIREQASGAYSVSSRLSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGI
                     SEQELNEYQQNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQE"
BASE COUNT          387 a          314 c          310 g          306 t
ORIGIN      
        1 caaatggtaa acaatgagct tgccgccaaa aaagcattgt ctcctgcggc tatcctggcg
       61 ctggaaaaag agtacgccaa caaaaagctg gcggcttaca tcttcccagg cagaaattta
      121 tcgttaacag tagacgctga cccacaggcg gaaattagca gcaaagaaac gctggctgag
      181 aatctgacat cattaacact ttccaatggt gccaaggtta ttctggcaaa atccgcgggt
      241 gaagagcaaa agctacaaat tattgccgta tcgaataaag gcgatttaag tttccctgcg
      301 cagcaaaaat cacttatcgc gctggcaaat aaagcagtta gcggaagcgg cgttggcgaa
      361 ctctcctctt ccagcctgaa acgctggagt gcggaaaact cggtaaccat gagcagtaaa
      421 gtcagtggca tgaatacgtt gctctccgtt agcgcgcgga ctaataaccc tgaacctggt
      481 ttccagttga ttaaccagcg aatcacccac agcacgatta acgacaatat ttgggcatcg
      541 ctacaaaatg ctcaaattca ggcgttgaaa acgctcgacc agcgtccagc ggagaaattc
      601 gcccagctga tgtatgagac gcgctatgct gatgaccgca cgaaattact gcaagaaaat
      661 cagattgcac agtttactgc cgcagatgcg ctggctgccg atcgccagtt gttttcatct
      721 ccagcggata tcacgtttgt cattgtcggt aatgtcgcag aagacaaact cgtggcgtta
      781 attacgcgtt acttaggctc aatcaaacac tctgattcgc cattagccgc aggtaaacca
      841 ttaactcgcg cgacggacaa cgcatcggtt actgtaaaag aacaaaatga acctgtggca
      901 caggtttcac agtggaagcg ttatgattcc cggacacctg ttaatctggc gacgcgtatg
      961 gcgctcgatg cttttaacgt cgcactggca aaagatctac gcgttaatat tcgtgaacag
     1021 gcatctgggg catacagcgt ttcttctcgc ctctcggttg atcctcaggc taaagatatc
     1081 agtcatttgc tggcttttac ttgtcaacca gaacgacatg atgaacagtt aacgttagcg
     1141 aatgaagtga tggttaagcg tttgactaag gggatcagtg agcaagaact gaacgaatac
     1201 cagcaaaacg ttcagcgcag cctcgatatc caacagcgta gcgttcaaca attagcgaac
     1261 actattataa atagtcttat tcaatatgac gatcctgcag catggactga gcaggag
//
LOCUS       DAJTSI010000348         1291 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.358, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000348 DAJTSI010000000
VERSION     DAJTSI010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.358"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..412)
                     /locus_tag="OO327_004288"
                     /pseudo
     CDS             complement(1..412)
                     /locus_tag="OO327_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(463..1038)
                     /locus_tag="OO327_004289"
                     /pseudo
     CDS             complement(463..1038)
                     /locus_tag="OO327_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1042..1291
                     /locus_tag="OO327_004290"
                     /pseudo
     CDS             1042..1291
                     /locus_tag="OO327_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          288 a          370 c          323 g          310 t
ORIGIN      
        1 catgggccat acacgcagct tccgttattc tgccggattc gtataacgcc cggtaaccac
       61 cgtaagcatc tgcctgaagc acaccgctgt aaccggctag gtgattctgt ggatggatac
      121 ctttccggtc cgggctgtac gcgaaccaga ccgccggggg catctgtgaa ccggcgttac
      181 ggtcatcacg gacatagacc cacagccggg cagttcttgt tttaccactg cccggctcct
      241 ggaccggaac ggggatatca tccgcatgaa ctttaccggg catcagtaca tactggcgca
      301 ggacgtcata cagcggctcc agcagttcag caacggcacc ggtccagcga cccagtgtgg
      361 cacggctcag ctccacgccc tgacgacggt atatttctga ctggcggtat aactgtcaac
      421 gacggatgaa aagtggctct tccgcaacct gggtggagat tttcatgcca gtggactcat
      481 taccctctgc ctgagtagtt caaatcctgc tcgaccatac atctggcgtt tcagcatctt
      541 cttcaggcga tttacatgac cttcgacaac accattactc cagcgactac tgattgcctc
      601 acatatagct gcagcatcag cttccatccc cgccgccacg cgccgaaatt ctgctgaacc
      661 gctttcgtgg actcgagtga accaactgct tagctgtgat ttattttggg ttttcagaat
      721 acggtagaac tcgagtgcca gttgctgcgc tattttcagc tcaggttctt tttcacacat
      781 cagactaata aatcgggaag cataattttc ttcatccctg gttattctcc agggcatcaa
      841 ccagcggctt acccgggaga ctgaaggaag tctgacggca gtagtaaccg gtgggttcca
      901 gcctttacgc catttggcaa ccgcatccct gacgatggtt tcactgcctg taaatccccg
      961 ggccaccatt tcccgccata tccggctggc gttataatta ccactttctc gttgctcttt
     1021 taaccactcc ctccatggtg tcagcgaaaa acgaaaccgc tgttgaaatc cctggaaagc
     1081 tggttgcgga aaagatgaag acccagtcgc gacactcaga gttggcgaag gcgttcgcgt
     1141 acgcacttaa ccagtggccg gcactgacgt actatgcgaa cgatggctgg gtggaaatcg
     1201 acaacaacat cgctgaaaat gccttgcggg cggtcagtct gggtcgtaaa aacttcctgt
     1261 tcttcggctc tgaccatggt ggtgagcggg g
//
LOCUS       DAJTSI010000349         1277 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.359, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000349 DAJTSI010000000
VERSION     DAJTSI010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.359"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..235)
                     /locus_tag="OO327_004291"
     CDS             complement(<1..235)
                     /locus_tag="OO327_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCR6020575.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLF"
     gene            complement(235..324)
                     /gene="kdpF"
                     /locus_tag="OO327_004292"
     CDS             complement(235..324)
                     /gene="kdpF"
                     /locus_tag="OO327_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR6020576.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            637..843
                     /locus_tag="OO327_004293"
     CDS             637..843
                     /locus_tag="OO327_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR6020577.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
BASE COUNT          397 a          283 c          265 g          332 t
ORIGIN      
        1 aaaacagcac cgccagcccc agcatgttca ggccgagaat ggcacaaaga tattgcttcc
       61 agttcatctc atggtcagag acgccaggta aacgaaagag tatacgttca acgcccgctg
      121 tacctggaag aggaatgtca ttaatcagcc gcgccagccc gctgcctaaa ggacgcgcca
      181 gcaccattaa taccagtaaa aacgtggcaa tcagtaagaa cccttgcgca gccatcagaa
      241 cgcctccgca ttgatcaggg cataaaccaa atagcccagt aataaaaaca ccagcaacac
      301 gccggctatc acgcctgcac tcacagtgca cctccagtgg cctaaaagtg ataccggaag
      361 agtagaattc tggctgcaaa gatttcgcaa aaatctgcgg acggggtgta aaaaaagtat
      421 aaaaatggca aaggccatga tttaactgat ggttagtatt aatttaactt ttgtgtaatt
      481 taattacagt gtgaatgtaa ataaaccatc aataagcaaa aataagtggt cggatgagta
      541 gtaaaattac acaaaaggcg gtactatttt catcagataa acaaattcat ttttccggtg
      601 ctattcaccg ggtagataca aaagggggag aaaagtatgg aactctacag agaatatcct
      661 gcatggctta tctttttacg ccgtacatat gcggttgcag cgggcgttct ggcgctgcct
      721 ttcatgcttt tctggaaaga ccgcgcccgc ttttacagct acctgcaccg cgtctggtcg
      781 aaaaccagcg ataaaccggt gtggatggat caggccgaaa aggcaaccgg tgatttttac
      841 tgattgcctg cgcgcttaag ccagcctcgt atcagggggc tggtttaggt gttcaacatc
      901 aacgtcttcc atgataatta acatggaaga cgtttccatc gtattattca tttcatcact
      961 gttttagcat cacgtttcaa tagtatcctt tcatagggct acccaggatg cggtcacctt
     1021 cattgtcgct ctaagcgact agatcatcag aaatctccag ttagattgca tacttcctta
     1081 cacactttcg tgaaacttca gggtaaggtg agaaagccgt catccctttt tctaattcca
     1141 gcatctaaat aaattccgaa ttttgttatt agtatcgcta ccgcaataaa aacagatagt
     1201 aaagagagta atatatactt tattgatata ttaagaataa ttaattgtta acatatctaa
     1261 tccaataaga tgttatg
//
LOCUS       DAJTSI010000350         1275 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.360, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000350 DAJTSI010000000
VERSION     DAJTSI010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.360"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            34..435
                     /locus_tag="OO327_004294"
     CDS             34..435
                     /locus_tag="OO327_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR6020578.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="OO327_004295"
     CDS             447..818
                     /locus_tag="OO327_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6020579.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..>1275
                     /locus_tag="OO327_004296"
     CDS             805..>1275
                     /locus_tag="OO327_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6020580.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNPPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIP"
BASE COUNT          303 a          286 c          396 g          290 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgccca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctttgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatctcgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccgggg tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atccgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtggag
     1261 gtagtgaaaa tcccg
//
LOCUS       DAJTSI010000351         1232 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000351 DAJTSI010000000
VERSION     DAJTSI010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.361"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="OO327_004297"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="OO327_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HCR6020581.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          365 a          206 c          294 g          367 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 cgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtgaatag cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact
      961 tcatatttga acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat
     1021 cgtaaaggaa accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt
     1081 cgatagcgcg agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat
     1141 tattcaatac aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt
     1201 ctctaaaaaa ctcgaaaaaa atggtaactg gt
//
LOCUS       DAJTSI010000352         1205 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.362, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000352 DAJTSI010000000
VERSION     DAJTSI010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.362"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            75..227
                     /gene="hokA"
                     /locus_tag="OO327_004298"
     CDS             75..227
                     /gene="hokA"
                     /locus_tag="OO327_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR6020582.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(414..626)
                     /gene="cspA"
                     /locus_tag="OO327_004299"
     CDS             complement(414..626)
                     /gene="cspA"
                     /locus_tag="OO327_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR6020583.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(907..1197)
                     /locus_tag="OO327_004300"
                     /pseudo
     CDS             complement(907..1197)
                     /locus_tag="OO327_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
BASE COUNT          328 a          250 c          252 g          375 t
ORIGIN      
        1 agtgaggcta ccctatgcct gaacaccata aggttagcct cttactcgtt ggaaatcaac
       61 acagggggct gggaatgccg cagaaatata gattactttc tttaatagtg atttgtttca
      121 cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat attaaacagg
      181 agagttatga gctggcggcg tttttagcct acaaattgaa agagtaagag tcttcgacgg
      241 gaaattattc ccgccttact tacggcgttg cgcattctca ttgcacccat atttattctt
      301 cacaaaaata ataatagatt ttattacgcg atcgattatt tatttcctga aaaaaaataa
      361 aaaaatcccc gccaaatggc agggatctta gattctgtgc ttttaagcag agattacagg
      421 ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa ggacactttc
      481 tgaccttcgt ccagagattt gtaaccatcg ttctggatag cagagaagtg tacgaacaca
      541 tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt gaaccatttt
      601 acgataccag tcattttacc ggacatagtg tattaccttt aataattaag tgtgcctttc
      661 ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact acgaggggta
      721 tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc tgtacgtcaa
      781 accgttgatg tgcattaagc cactcattgg cgggtgatgc aacaattatt tttcatattt
      841 atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa taaagtggat
      901 aaaagtctat ttcatcaact gcttacttaa tgccgggttg gcttgaatca aacgcatcaa
      961 ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta ccatggcgac
     1021 tgatacgccc aaaacccggg cgaaatcatc gatttttaat cctgtccctt ttcataactg
     1081 ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg tttcatcttt
     1141 aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat attccattga
     1201 aaact
//
LOCUS       DAJTSI010000353         1204 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.363, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000353 DAJTSI010000000
VERSION     DAJTSI010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.363"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..218
                     /locus_tag="OO327_004301"
     CDS             <1..218
                     /locus_tag="OO327_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HCR6020584.1"
                     /translation="LNIDIASPGTKINEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAI
                     AELHGGSATYHYLSKHNVFRITLPQRN"
     gene            complement(326..1177)
                     /gene="hchA"
                     /locus_tag="OO327_004302"
     CDS             complement(326..1177)
                     /gene="hchA"
                     /locus_tag="OO327_004302"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCR6020585.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
BASE COUNT          315 a          275 c          288 g          326 t
ORIGIN      
        1 ctcttaatat tgatatcgcc agtcctggaa cgaaaattaa tgagcctgaa aaactcttcc
       61 gtagattttg gcggggagat aattcgcgtc attccgtagg tcagggactt ggcctttctt
      121 tagtcaaagc gattgccgaa ttacatggag gaagtgctac gtatcactat ctcagtaagc
      181 ataacgtgtt ccgaattacg ttaccgcaaa gaaattaaca tcgggcttca gtacgccata
      241 agttaaccgt gaaaaaacgc agccacgtcg tgagtattaa cgtggctgcg attgattatg
      301 cgcttacatc acaatgtaac agggattaac tcgcgtaagc tgccagcatt tcctgcgccg
      361 ccagtttacc caacgcattc gctgcaaaag gactgtcgcc ggtgagacgt ttacggtcct
      421 tatgtactcg cccggtgatg tcgtcattaa tgatgttcat gcccattttc ttcaactctt
      481 cgccgaagta ccaggtgaga tgacccggca tatagccaat atctggcgtt tgtttgtctg
      541 cggcgtctgg gaatgcgcaa atagaataac cattcagtgg gttatcgctg tggcgaagtg
      601 ccagaaaagc cgccggaccg tggcaaaggg agataacaaa acggtcattt ttgattgccc
      661 actgtaaagc ggcagccacg tcctggcttt ctggtaaacc aataagtgcg ccatgcccac
      721 caggaacaaa gattgctgca tattcgctat cagcgttgag gctggcgaca acatccgcga
      781 gtttcttcgg attacggaat aacgatttat gctgttcaaa gaatggcatt actttttcat
      841 ctttgtgcgg catagcccag tattcaaact tggtcatcag accggaaatg gtcgccactt
      901 cgaattcgaa acctgcagca tggagatgat acaacggcag caacgtttca atcggatggt
      961 taccggtcga gaacagtttt ccgttatcgg tcggcaaata acgttcgtcc gcggcgatca
     1021 ccagaatttt atgtttaccg cgatacggtt ttggatagtc cacgccatca agatcagaga
     1081 cgggactcgt atattggcta agcgaatatt ctgaagggaa gaacgcatta tcttcagcaa
     1141 tatcgacctg cgggttttta cttgtttgaa cagtcatagt gtattcctta ttgttgctta
     1201 gtta
//
LOCUS       DAJTSI010000354         1189 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.364, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000354 DAJTSI010000000
VERSION     DAJTSI010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.364"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(267..1175)
                     /locus_tag="OO327_004303"
     CDS             complement(267..1175)
                     /locus_tag="OO327_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020586.1"
                     /translation="MTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRILAERQIQE
                     TEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEARANL
                     ALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQA
                     AAIVELAEATHKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEP
                     MKSIDGIKIIQVDGLNSGSAAGDANTGNVGGGNLAEQALSAALSYRTQAPLIDSLLNE
                     IGVSGGSLAALTSPLISTTPVAENVK"
BASE COUNT          228 a          324 c          289 g          348 t
ORIGIN      
        1 caatcagtat gatgacgagc ttcatcataa ccctttcctt ctgtaaggcc ccttcctcgg
       61 gaggggcttt cccgtttcag ccctttgctg actccccgat tgttgatgag cgccggacct
      121 cgcgcaacgc tttcccagag gcagttttgc cttccccgag cggtcacgca aaacgttgca
      181 acgacctgat catttgcagt ttttagcgga acgcagatat aaaaaatccc cggctttttg
      241 tgccagggac gttcatagct gaaaatttat tttacgtttt cggcgacggg agttgttgag
      301 attaagggtg aagtcaatgc cgccagtgaa ccgcctgaaa cgccaatttc attgagcaag
      361 gagtcaatca gcggagcctg tgtgcggtaa gagagagcgg ctgataatgc ttgttccgct
      421 aggtttccgc cccccacatt acccgtgttt gcatcacccg cagcgctgcc actattcaga
      481 ccatcgacct gaataatctt gataccgtca atagatttca tcggctcaac ggatttttct
      541 attaccgcag gtagcgcctg caacaaggcc agtttgaatt taaggctggt ttgttcatca
      601 gaaagtacgt tgatagcatc gttcagcgca cgttgtgctt ctgcttccgc cagacccttt
      661 ttatgtgtag cttcggctaa ctcaacgata gccgccgcct gcatctctgc ggcttctttt
      721 tctgctttcg cccgcacggt cagttcaacc gctttggttt ctgcatcctg cgcggcagcg
      781 attagggcaa cttgtttagc acgatcggct tctgcggtct ggcgagtggt ttctacgttt
      841 tgttgcgcac ttactgcttc tgcgagtgca aggttagcac gcgcttctgc ctgggactgt
      901 tgttccgatt tggcggcaat agcgatcgat ttcgtctggt tggcgatttc ggtgacctgc
      961 tgctgttcga tctctttaat acgaacttca cgttcagcct caacctttct tgagcggacg
     1021 gcctgctcgc gctcgatctc cgtttcctga atttgtcgtt cagccagaat tcgcgtctgc
     1081 tctgcttcac gacgacgttc agcttcaaaa gccgcaatac gtgcattctg ttcggcagta
     1141 cgggttttaa cctgctgctc ctgctcaagc gtcataaacg cttcttgtt
//
LOCUS       DAJTSI010000355         1159 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.365, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000355 DAJTSI010000000
VERSION     DAJTSI010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.365"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            115..>1159
                     /gene="cadB"
                     /locus_tag="OO327_004304"
     CDS             115..>1159
                     /gene="cadB"
                     /locus_tag="OO327_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HCR6020587.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKAS"
BASE COUNT          246 a          255 c          298 g          360 t
ORIGIN      
        1 tttaggatta atcctttttt cgtgagtaat cttatcgcca gtttggtctg gtcaggaaat
       61 agttatacat catgacccgg actccaaatt caaaaatgaa attaggagaa gagcatgagt
      121 tctgccaaga agatcgggct atttgcctgt accggtgttg ttgccggtaa tatgatgggg
      181 agcggtattg cattattacc tgcgaaccta gcaagtatcg gtggtattgc tatctggggt
      241 tggattatct ctattattgg tgcaatgtcg ctggcgtatg tatatgcccg actggcaaca
      301 aaaaacccgc aacaaggtgg cccaattgct tatgccggag aaatttcccc tgcatttggt
      361 tttcagacag gtgttcttta ttaccatgct aactggattg gtaacctggc gattggtatt
      421 accgctgtat cttatctttc caccttcttc ccagtattaa atgatcctgt tccggcgggt
      481 atcgcctgta ttgctatcgt ctgggtattt acctttgtaa atatgctcgg cggtacctgg
      541 gtaagccgtt taaccactat tggtctggtg ctggttctta ttcctgtggt gatgactgct
      601 attgttggct ggcattggtt tgatgcggca acttatgcag ctaactggaa tactgcggat
      661 accactgatg gtcatgcgat cattaaaagt attctgctct gcctgtgggc cttcgtgggt
      721 gttgaatccg cagcagtaag tactggtatg gttaaaaacc cgaaacgtac cgttccgctg
      781 gcaaccatgc tgggtactgg tttagcaggt attgtttata tcgctgcgac tcaggtgctt
      841 tccggtatgt atccgtcttc tgtaatggcg gcttccggtg ctccgtttgc aatcagtgct
      901 tcaactatcc tcggtaactg ggctgcgccg ctggtttctg cattcaccgc ctttgcgtgc
      961 ctgacttctc tgggctcctg gatgatgttg gtaggccagg caggtgtacg tgccgctaac
     1021 gacggtaact tcccgaaagt ttatggtgaa gtcgacagca acggtattcc gaaaaaaggt
     1081 ctgctgctgg ctgcagtgaa aatgactgcc ctgatgatcc tcatcactct gatgaactct
     1141 gccggtggta aagcatctg
//
LOCUS       DAJTSI010000356         1146 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.366, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000356 DAJTSI010000000
VERSION     DAJTSI010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.366"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            251..>1146
                     /locus_tag="OO327_004305"
     CDS             251..>1146
                     /locus_tag="OO327_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6020588.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGG"
BASE COUNT          312 a          256 c          279 g          299 t
ORIGIN      
        1 tttttcgcac ttttgacttt gaaacctcta ttacgtcaac ccttccgcac tcgggagggt
       61 tctactgtta tagggatttt agctcactct ttcaacgagc aattgtatat tgttatgtaa
      121 gcaagtgctt acacctgtca gaaatttgct cattattgag aacattaaat cggatgacat
      181 atcacgcatc ctgatggttt aatgacaaat aaattcaaaa tatctcatct attttgcaac
      241 aggagcaaaa atgaaagcca cgtcggaaga actcgccatt tttgtttcgg tcgtcgaaag
      301 cggcagcttt agccgggcag cggaacaatt agggcaagca aactcagcgg taagccgggc
      361 ggtgaaaaag ctggaaatga aacttggcgt tagcctgctt aatcggacca cgcggcaact
      421 tagcctgacg gaagaaggcg agcgttattt tcgtcgcgta cagtcaattt tgcaggagat
      481 ggcagcggca gaatcagaaa ttatggagac gcgtaataca ccgcgtggac tgttacggat
      541 cgatgccgca actccagtgg tgctgcactt tctgatgccg ttaattaagc ctttccgtga
      601 acgctatccg gaagtcactt tatcgctagt ctcctccgaa acgattatta atttgatcga
      661 aagaaaagtg gatgtcgcga tacgcgctgg tacgttaacg gactccagct tacgtgccag
      721 gccgttattt aacagttatc gaaaaattat cgcctccccc gattatattt cccgctacgg
      781 gaagccagaa acgatcgacg atttaaagca acatgtttgc ctgggattca ctgaacccgc
      841 ttccctcaat acctggccga tagcctgtag tgatggacaa ttacatgagg tgaagtacgg
      901 tttgtcatcc aatagtgggg aaacactgaa acagctttgc ctgagtggca acgggattgc
      961 gtgtttgtcc gactacatga tcgacaaaga aatcgctcgc ggagaattgg tggagttaat
     1021 ggcagataaa gtgttgccag tggaaatgcc attcagtgcc gtctattaca gcgaccgtgc
     1081 ggtaagtacg cgcatccggg cttttatcga tttccttagc gagcatgtaa aaacagctcc
     1141 cggagg
//
LOCUS       DAJTSI010000357         1140 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.367, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000357 DAJTSI010000000
VERSION     DAJTSI010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.367"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..730
                     /locus_tag="OO327_004306"
     CDS             <1..730
                     /locus_tag="OO327_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCR6020589.1"
                     /translation="TDRIHLKDAARMLEVSVMTIRRDLHQEDEPLPLTLLGGYIVMVN
                     KPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDNGQEIPLVISMIPDAITFT
                     GICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNSSPLDSLNPRKIFISASGV
                     HNHFGVSWFNPEDLATKRKVMNRGLRKILLARHALFDEVASASLAPISAFDVLISDRP
                     LPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(732..958)
                     /locus_tag="OO327_004307"
                     /pseudo
     CDS             complement(732..958)
                     /locus_tag="OO327_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229544.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT          298 a          283 c          247 g          312 t
ORIGIN      
        1 aaccgacaga atccatttga aagacgcagc gcgaatgctg gaagtttctg taatgactat
       61 tcgtcgcgat ctccatcagg aagatgaacc tctgccactg accctactgg gtggctatat
      121 tgtaatggtg aataaacccg cgccatccat gccagtaatc catgacgttc caaaaaatca
      181 tcgtgatgac ttacctattg caattctggc tgccggaatg gttaatgaaa atgatctgat
      241 cttctttgat aatggccagg agataccact cgttataagc atgatcccgg atgcaatcac
      301 cttcaccggc atctgttact cacatcgcgt ctttgttgcg ttgaatgaaa agcctaatgt
      361 aacagcaata ctttgtggtg gtacgtatcg tgccagaagt gatgcttttt acgatgccag
      421 taactcttcg ccattagact ctctcaatcc gcgaaaaata tttatttccg ccagcggtgt
      481 gcataatcac tttggcgtca gctggtttaa ccctgaagat cttgccacta agcgtaaagt
      541 gatgaaccgt ggactacgga aaattttgct cgcccgccac gcgttgttcg atgaagtggc
      601 ctctgccagc ctcgcaccga tctctgcatt tgacgttctg attagcgatc gtccgttacc
      661 ggcagattat gttacgcact gccagaatgg ttctgtaaag atcattacac ctgattcaga
      721 agacgaatga cttactgaaa aaacaccaca atcttgttaa acatcgtcgg gttggactga
      781 ttacgttgca ctttcaccac atattccagc ttatctattt ggcttatcac ctactccaga
      841 cgctggtcat ccttgaccag tagccagata tggcttttgt cgcagtcctg aatcggcagg
      901 cagagaatgt cttcaacgtt aagagcgcga cgggcaaaag cccacaaagg tgagtcatta
      961 cacccggatg gtaaacaggc acttccttgt tgtagaagcc gttatattgt ccataatgtc
     1021 tgggttttta atgcatactg ggtaacaacc ggacgaatct tcgaatatca ctggcgctgc
     1081 cagccggttt gaattcatct cacaaccctg catagagcga atctcctgcc tgcacgtcac
//
LOCUS       DAJTSI010000358         1139 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.368, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000358 DAJTSI010000000
VERSION     DAJTSI010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.368"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            79..351
                     /locus_tag="OO327_004308"
     CDS             79..351
                     /locus_tag="OO327_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020590.1"
                     /translation="MVRKFDELEAKCAAQENKVIPVSTELPPANESVLLFDANGEGWL
                     IGWRSLWYTWGQKETGEWQWTFQVGDLENVNITHWAVMPKAPEAGA"
     gene            351..656
                     /locus_tag="OO327_004309"
     CDS             351..656
                     /locus_tag="OO327_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020591.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            656..1006
                     /locus_tag="OO327_004310"
     CDS             656..1006
                     /locus_tag="OO327_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6020592.1"
                     /translation="MEPYSLTLDEACHFLKISRPTAINWIRTGRLQATRKDPTKNKSP
                     YLTTRQACIAALQSPLHTVQVSAGDGITEERKCHSSAEVKYGTPVSHCRTVKDLNSLL
                     EQRTKGRRQNSMTS"
BASE COUNT          367 a          247 c          285 g          240 t
ORIGIN      
        1 cagcctgagt tcacagataa aacactctcc aggaaacccg gggcggttca gaaagtaaat
       61 gaaacagatg ctgaatacct ggtgcgtaaa ttcgatgagc ttgaagctaa atgtgcagca
      121 caggaaaaca aagtaatacc agtgtcaact gaactgccac cagcaaatga aagtgttttg
      181 ttattcgatg ctaacggaga aggctggcta attggctggc gttctctctg gtacacctgg
      241 ggacaaaaag aaaccggaga atggcagtgg acatttcagg tcggggacct tgaaaacgtc
      301 aatatcactc actgggcagt aatgccaaaa gcaccggagg ctggagcata atgaccactt
      361 ttaccgacaa agaactgatt aaagaaatta aagagcgtat cagcagcctt gacgtgcgag
      421 acgatattga gcgccgtgct tatgaaatcg cactcctatc tctggaagta gaaccagatg
      481 aacgcgaagc ttatgaatta ttcatggaaa agcgttttgg tgacttagta gatcgtcgga
      541 gagcaaaaaa cggcgataac gaatacatgg catgggatat gactctcggt tggatcgtct
      601 ggcagcaacg agctggtatc catttctcaa caatgtcaca gcaagaggtg aaataatgga
      661 gccatacagc ctcacactcg atgaggcctg tcattttctc aagatatcca gaccgactgc
      721 cattaactgg atacgcacag ggcgtcttca ggcaacacgc aaagatccca ctaagaataa
      781 atctccttac ctcacaacac gacaagcctg cattgcggct cttcagtctc cgctgcatac
      841 tgtccaggtg agcgcgggtg atggcataac agaggaaaga aaatgtcact cttccgcaga
      901 ggtgaaatat ggtacgccag tttcacattg ccgaacggta aaagatttaa acagtctctt
      961 ggaacaaagg acaaaaggca ggcgacagaa ctccatgaca agctaaaggc tgaagcatgg
     1021 cgggtcagca aacttggtga aatacctgat ataacgttcg aggaagcgtg tgtcaggtgg
     1081 cttgaagaga aagcacataa aaaatcactg gacgatgaca aaagccggat cggattctg
//
LOCUS       DAJTSI010000359         1109 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.369, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000359 DAJTSI010000000
VERSION     DAJTSI010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.369"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1019
                     /locus_tag="OO327_004311"
                     /pseudo
     CDS             1..1019
                     /locus_tag="OO327_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          312 a          271 c          258 g          268 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc atcaggtta
//
LOCUS       DAJTSI010000360         1100 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.370, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000360 DAJTSI010000000
VERSION     DAJTSI010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.370"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            55..>1100
                     /locus_tag="OO327_004312"
     CDS             55..>1100
                     /locus_tag="OO327_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR6020593.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
BASE COUNT          344 a          255 c          178 g          323 t
ORIGIN      
        1 cattaggaga gatacattaa atacaattta ttcacaatct aattccatat ccatatgcga
       61 gaaattaata tgctcagaaa catttcatcc tgtttatttc cacatatcag cacaattaca
      121 tcccccaacc attatttgtc cgaatgggat gattgggaga aacaggggtt accggaagaa
      181 cagcgtactg aggcggtaag aagacttcgt gcatgtctta cctctaaggg gcataaactg
      241 gacctgcgag ccttggcgct ttcctcgcta cctgtactcc ctgcttgcat taaaaagctt
      301 gatgtgagct gtaataaatt aaccatcctt actgatttac ctgaaaatat taaagaactt
      361 attgcaagag ataatttctt aacacatata tctgcattac cacattatct aataactttg
      421 gatgtgtccg aaaatcaatt agagaatctg ccgttattac cagacaccat caaatcacta
      481 agcgcagagt ataataggtt atccacactg ccttcattac ccttgaattt aaaaaaactt
      541 gaggttagga acaacgaact gcaaactctt ccatctctgc cttctaatct taagatactt
      601 aaggttgcgc acaaccatct tactgaactg ccccctttac ctaggagact gcaacttctt
      661 tttgcatata gcaatagatt aagcaactta ccaaacatcc aagaaaatat tatcatgaga
      721 agattttttt attttgaaaa caaccaaata actacaatcc cgacaaatct ttttcgttta
      781 gatcctcata taactattga gattgcaaat aaccccttat cagatcaaac tctgctattc
      841 ttaatacagc aaacttcggt tccaaatttt aacgggcctc agtttcgtat ttccctgtca
      901 gaccaaaaca gactgttttt acgccagatg ttgccgcaaa atttacattc gcgccatatc
      961 agagtcatca ctgaaggggg gcagaacttt cagatccccc ctcttcccga aactgtggca
     1021 gcctggtttc ctgaagcaga tcgtcgggag gtttctacac aatggacttc tttttccacc
     1081 gaggagaatt cccgggcatt
//
LOCUS       DAJTSI010000361         1095 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.371, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000361 DAJTSI010000000
VERSION     DAJTSI010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.371"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(57..326)
                     /locus_tag="OO327_004313"
     CDS             complement(57..326)
                     /locus_tag="OO327_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6020594.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            383..1051
                     /locus_tag="OO327_004314"
     CDS             383..1051
                     /locus_tag="OO327_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6020595.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
BASE COUNT          306 a          250 c          223 g          316 t
ORIGIN      
        1 accagccaac cgcagcacgt cctgcatacg tcgtgtctgc ggtttttctt tttcgcttac
       61 attgtgtctg gttcttccgg ccactcaata tcaggtgcag ttgatgtatc aacacggttc
      121 agcaacaccc gatacttctt ccaggcttcc agcaacgagt tttcttcctc cgttgcgatc
      181 tccagatcta cagcatcctg aagtggcgca atatgctcac tgaatccctg gatgtagaac
      241 tgtgtggtga cggtcttcca gccattcggc tcctgctgta tcgaagcata ccaggctatt
      301 tcaatatcgc tatgctgcgg cagcatttaa ccccttgtaa ttcatcgcca taattgattt
      361 aattcacaaa taaaactata atatggtgaa attaatgaaa aaaaacacag atgatggggc
      421 taaaatttac acaccactta ccctaaagct ttatgactgg tgggttttgg gagtatcaaa
      481 tcggcttgca tggggatgtc ctacaaagga acaccttctt ccacactttc tggaacattt
      541 aggtaacaac catctggata ttggtgttgg aactgggttt taccttactc acgtacctga
      601 gagtagtctg atatctttaa tggatttgaa cgaagctagc ctgaacgcgg catctacaag
      661 ggctggggaa tcaaaaatta aacataaaat tagccatgat gtttttgaac cttatcctgc
      721 ggcgttacat ggtcaatttg attccatttc catgttttac cttcttcact gcctgcctgg
      781 aaatatatct acaaaaagct gtgtaatacg caatgcggcg caggccttaa ctgatgatgg
      841 aactctatac ggagccacaa ttcttggcga tggagttgtg cacaatagct tcggtcaaaa
      901 actgatgcgc atttacaatc agaaaggcat cttttcaaac acaaaagatt ccgaagaagg
      961 cttaacacat atactctcag agcatttcga gaatgttaaa accaaggttc aaggtactgt
     1021 agtaatgttt tccgcttcag ggaaaaaata gcatccaacc gcagcacgtt cttgcttaag
     1081 acgtgctacg gtttc
//
LOCUS       DAJTSI010000362         1095 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.372, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000362 DAJTSI010000000
VERSION     DAJTSI010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.372"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>1095
                     /locus_tag="OO327_004315"
     CDS             <1..>1095
                     /locus_tag="OO327_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6020596.1"
                     /translation="QEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMGLRFGRDV
                     NAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVITEEWRAH
                     QDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIV
                     VGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYY
                     RLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPDYQS
                     LFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERD
                     LRMLTSRLASSSLNNTPFLSPEETYQLSKRL"
BASE COUNT          315 a          250 c          269 g          261 t
ORIGIN      
        1 caggaagaag acaatgagcg cggcgtggct cattttgtag aacatatgat gtttaacggc
       61 acaaaaacat ggccgggtaa taaagtcatc gaaacatttg agtcaatggg cctgcgtttt
      121 ggtcgcgatg ttaatgccta taccagctat gacgaaacgg tgtatcaggt gagtttgccg
      181 actacgcaga aacaaaatct gcaacaagtg atggcaatct tcagtgaatg gagtaatgcc
      241 gcaacctttg aaaaactcga agtagacgct gaacgtggcg taattactga ggaatggcgt
      301 gcccatcagg atgcgaaatg gcgcacctct caggcgcgcc gccctttcct gctggctaat
      361 acccgtaatt tagaccgtga acctatcggc ctgatggata ctgtcgccac ggtcacgccg
      421 gcacaattgc gccaatttta tcaacgctgg tatcaaccaa ataatatgac ctttatcgtg
      481 gtcggcgata tcgacagtaa agaagcgctg gcgctgataa aggataattt aagtaagctt
      541 ccggctaaca aagcagctga aaatcgcgtc tggccgacaa aagccgaaaa ccacctgcgc
      601 tttaatatca ttaatgataa agaaaaccgg gtgaacggca tcgcactcta ttatcgcctg
      661 ccaatggtac aagtgaacga tgagcaaagc tttgtcgaac aagctgaatg gagcatgtta
      721 gttcagctgt tcaatcaacg tctgcaggaa cgcatacagt cgggcgagtt gaagactatt
      781 tctggcggca ctgcgcgcag cgttaaaatt gcacccgatt atcagtcgct gtttttccgt
      841 gtaaatgcac gagacgataa tatgcaggat gctgcgaatg cattaatggc agagttggca
      901 accattgatc agcatggttt ttctgctgaa gaactcgatg atgtcaaatc tacccgcctc
      961 acctggctga aaaatgcggt tgatcagcaa gctgaacgtg atttacgtat gctgaccagt
     1021 cgcctggcat ccagctcatt aaataatacg ccgttcttgt cgccggaaga gacatatcaa
     1081 ctttcgaaac gtctg
//
LOCUS       DAJTSI010000363         1080 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.373, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000363 DAJTSI010000000
VERSION     DAJTSI010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.373"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            41..817
                     /locus_tag="OO327_004316"
     CDS             41..817
                     /locus_tag="OO327_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR6020597.1"
                     /translation="MTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGK
                     TDVPIFNTKDMRLGIGLHLIDFIRKSKDQGFREFCYNKNIDPVSLDRIINFVFQLEYH
                     IPRMLSTDNFKKIKLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNKKADIA
                     LYLLSKFNFTKQDVAEMEKMKNNRYCNLYDVEYLLSKDGANYKVLEYFINNGLVDVNK
                     KFQKVNSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          373 a          167 c          210 g          330 t
ORIGIN      
        1 caatgcgtat atcattgatc atgactctcc atatggatat atgacattaa ccgatcactt
       61 tgataatgct attccacctg ttttttacca tgagcaccaa tcatttttct tagataactt
      121 taaagaggtt gtggatgaag ttagcaggta tgtgcatggt aatcagggga aaactgatgt
      181 tccaatattt aacactaaag atatgaggct tggaattggg ttgcatctta ttgattttat
      241 aagaaagagt aaagatcaag gattcagaga gttttgttat aacaaaaata ttgatcctgt
      301 tagtctggat agaattataa actttgtgtt tcagcttgag tatcatatac caagaatgct
      361 aagtacagat aacttcaaaa aaataaaact cagagacata tctttggagg atgcgattaa
      421 agcatctaat tatgaagaga ttaacaataa agtgactgat aaaaaaatgg ctcatcaggc
      481 ccttgcttat tctttaggca ataaaaaagc tgatatagca ctttatttac tatctaaatt
      541 taattttaca aaacaagatg tcgcagagat ggagaaaatg aaaaacaata gatattgtaa
      601 tctgtatgat gttgaatact tacttagtaa agatggtgcc aactataaag tgctagaata
      661 ttttatcaat aatggattgg ttgatgtaaa caaaaaattt caaaaagtaa atagtgggga
      721 cactatgctt gacaatgcaa tgaaaagcaa agattcaaaa atgattgatt ttttattaaa
      781 aaatggagca atattaggca aacgatttga gatatgacct aaaagctcgg ccaaataata
      841 atgtcgtcga ttatcgaagt catcatgagt gattctgtcc ccacagtgca gcatcagcat
      901 gacagtgaat tgtctggcgt ggtccattta atgagatgga gatggtccct ctttcctaaa
      961 tagctgtgcc taataccgct acacttttgc cagcccatgt ttgcctccgg ggaatgggct
     1021 gacggttcct tcaaaatcca ttgcacgcag ttatgtcgga gcgggaactt gactcctgtc
//
LOCUS       DAJTSI010000364         1069 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.374, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000364 DAJTSI010000000
VERSION     DAJTSI010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.374"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(348..1043)
                     /gene="aqpZ"
                     /locus_tag="OO327_004317"
     CDS             complement(348..1043)
                     /gene="aqpZ"
                     /locus_tag="OO327_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HCR6020598.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT          300 a          321 c          250 g          198 t
ORIGIN      
        1 gatttttaat cctatctgag tgtttagcat tgttgcgctc ctttcatgtg caatgccata
       61 taaagccact ggcggaggga gcgcgcaggg ggcagccagt cgccgccgcc ccctgcaccc
      121 ccgggctctg gcgaacaaaa tcgccgctgc gcggtacctt cagcttatcc ccttcggcaa
      181 ccgggtcggg cgcgaggtaa cgtcctgtaa gacgcgccct cagcccacat ccatgtgggc
      241 tgccccggcc ttcggggaac gcttcagcga ttttgacgcc accaatacct gtgcggtttg
      301 taatgtcgaa gataaaacaa ccagggcaga taaaatgcca atgctaatta atcacgcttt
      361 tccagcagag tccggtaaat cagaccaccg ataatgccgc cgacaattgg caccacccag
      421 aagaaccaca gttgttctaa tgcccagccg ccctggaaga tagcaaccgc ggtgctgcgc
      481 gccgggttaa cagaagtgtt agtcaccgga atactgatta agtgaatcag ggttaaggcc
      541 agaccaatag cgatcggcgc aaaacctgcc ggagcgaatt tgtcggttgc accgtggatc
      601 actaacagga agcccgcact caataccagt tcaactacca gcgcggaaag catggaataa
      661 ccgcctggtg aatgctcgcc ataaccgtta gaagcaaaac cgctggctgc cgcgtcaaaa
      721 cccgttttac cactggcaat taaatacagc agcgccgctg caacaatacc gccgacaacc
      781 tgggcaatta cgtagccaac gacttctttt gccggaaaac gtccgccagc ccataaacca
      841 atagtgaccg ccgggttaaa atgaccacca gaaatatgac caacagcgaa ggccatcgtc
      901 agaacggtca aaccaaatgc cagcgccacg ccggcaaaac caatgcctaa ttccgggaag
      961 cctgcggcca gtacagcact accacagcca ccaaaaacaa gccagaaagt accaaaacat
     1021 tcagctgcta attttctgaa catatccacc tcaattaaaa attgaccct
//
LOCUS       DAJTSI010000365         1067 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.375, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000365 DAJTSI010000000
VERSION     DAJTSI010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.375"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(226..651)
                     /locus_tag="OO327_004318"
     CDS             complement(226..651)
                     /locus_tag="OO327_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020599.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
     gene            complement(648..1031)
                     /locus_tag="OO327_004319"
     CDS             complement(648..1031)
                     /locus_tag="OO327_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020600.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDTLPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
BASE COUNT          252 a          307 c          265 g          243 t
ORIGIN      
        1 tcgcgtatgg ttgattgaca ccacagtgcg aattcggcga atgcgagatc acggtacgat
       61 tccgcaccac actaccaatc aataccccgg gttttattca ggtaagcagg actgcggata
      121 tccggtgtcg cgcctttgtg tcacgaacgg ggtgggtgcg aaacaccaga taaaatacag
      181 gttggcaata cctctgaacg ccctgagcag gtcgggcatt tcggattaag tactcgcacc
      241 tccgcagtcc tcaaacaagt ctggctggta gctgtaaaca gacttcgtac ataccgctct
      301 gcaataaatc cccgtaccac aggcctcaca gaacttcttc ccatgaaaaa actgctcaca
      361 catcacacaa tgccactcca gcacgaccgg taatggcgat aaaaacatcg ccatatcctc
      421 aatgtaaggg tgggacttct ccggattcag caccacacag tccgccttct gttgctcact
      481 cagggcatgt aaatcatgct caaaccacgc cccctgagca tctgtctgca gaatcaaccg
      541 gccatggcag gaaaggcaga aacactgccg gatatttctg ctgagcgcag agtcggcact
      601 gataatgtgc tcacccggcg tgatccccag ccccgttttt ataccgttca ttctgctccc
      661 ccctcctcac tgaagcgccc ggtctggcag tgtgtgcagt actgctcccc ataataatcg
      721 tggtgacatt gcctgcagtg ccagctggct ttacgtacta cgggtaaggt atccgggacg
      781 aattgctgca gacgtttaat caaccgtatt tctctgcgtt ccggcctgac ataagggcac
      841 tcatgaccgt gctccgtcag cccttcgtca gcgtgttcaa accagggccg ttcagtgtcg
      901 tattgcgggt ggtatctgag tgcactgccg caaaggtggc aggtgtagct atcgtaaggt
      961 gcagtctgtg cggtacgggc accggtcaga cgtccgttgc catcaagtgc gagaaaagat
     1021 tttgcgtaca tagtatatgt tcctcagccg ccagacgaca cgcaggc
//
LOCUS       DAJTSI010000366         1023 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.376, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000366 DAJTSI010000000
VERSION     DAJTSI010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.376"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..553
                     /locus_tag="OO327_004320"
                     /pseudo
     CDS             1..553
                     /locus_tag="OO327_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          313 a          224 c          217 g          269 t
ORIGIN      
        1 caggtctggg tcgcagattc agttgtccag gccttccgaa atcaaccgac tgaaggcgct
       61 ggtcgcgaaa ctgcagcgta tgcagttcgg taaaagctca gaaaaacttc gcgcaaaaac
      121 cgaacggtgg atacaggaag cacaggagcg aatcagcgca cttcaggagg aaatggcgga
      181 aacactgggt gagcaatatg acccggtact gccatcttcc ctgcgccagt cttcagcccg
      241 taaaccgtta ccagcctcac ttccccgtgc cccccgggtc atccggccgg aagaggaatg
      301 ctgtccggcc tgtggtggtg aactcagtcc actgggatgt gatgtgtcag agcagctgga
      361 gcttatcagc agcgccttta aggttatcga aaaacaacgc cctaaactgg cctgtcgccg
      421 gtgcgaccat atcgtgcagg caccggtgcc ttcaaaaccc attgcacgca gttatgccgg
      481 agcggggctt ctggcccatg ttgttaccgg aaagtatgca gaccatctgc cattataccg
      541 ccaaagtgat ctactattcc acacagctat ttagctctct ttttgttgag ctcccatgat
      601 tctttcattt attgttacta attattttat gttatcatga tgttagaaca cgctctgctc
      661 tgtgtctcta ttttatctat catattctga ggattaacta cataacaagg cgcaagatat
      721 atatacaaaa acaaataata ccgttgcaat aattcctttt agtaaaggag gtgtgattaa
      781 tttcagggga tttcagagta aaaattgtac aacatattaa aatatgttca tttcgttata
      841 atacacacaa ggaaatatta agttagataa tatattctaa ataaccatag ataaaaacgc
      901 acataatggg gaatatttta tgaaaatacc tgaagcagta aatcatatta atgttcagaa
      961 taatattgat cttgttgatg gaaaaacgaa tccgaataaa gcaacaaaag cattacaaaa
     1021 aat
//
LOCUS       DAJTSI010000367         1019 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.377, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000367 DAJTSI010000000
VERSION     DAJTSI010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.377"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(50..190)
                     /locus_tag="OO327_004321"
     CDS             complement(50..190)
                     /locus_tag="OO327_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020601.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            329..1000
                     /gene="queE"
                     /locus_tag="OO327_004322"
     CDS             329..1000
                     /gene="queE"
                     /locus_tag="OO327_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCR6020602.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          295 a          205 c          237 g          282 t
ORIGIN      
        1 acatacgaaa gtgaacatta gtatgaaata gcgcattata aatttctcat taaatctgga
       61 taaataaatt gacggcggca tcggtaattt tagattcgtt atcattaaaa actaatacag
      121 aaaaaataaa tataattttg gtgaatgttt tgacatgatt aaatccattt tctttatttt
      181 cttctgacat aatacgttac tccctttaat tattaacgat atgtttcgta tttttcttta
      241 aatctgcatg taacagaatg ttaataagac atgattgttg cggcaggatc tgcaataatt
      301 aacaattatc cacttcacag agaatgctat gcagtacccg attaacgaga tgttccagac
      361 cctgcaaggt gagggttact ttaccggcgt tcccgccatt tttattcgtt tacagggatg
      421 cccggttggc tgtgcctggt gcgacaccaa acacacctgg gaaaagcttg aggatcggga
      481 agtctccctt ttcagcattc tggcgaagac caaagagagt gataagtggg gggcggcgag
      541 cagtgaagac ttgctggctg tcattggtcg tcagggatat accgcgcggc atgtggtgat
      601 tacgggtggt gagccttgca ttcatgattt gctgccactg actgacctgc tcgaaaagaa
      661 cggttttagc tgccagatcg aaaccagtgg aactcatgaa gtacgctgca caccgaatac
      721 ctgggttacc gtatcgccaa agctgaacat gcgcggcggc tatgaagtgt tgtcacaggc
      781 actggagcga gccaacgaaa tcaagcatcc agtggggcgc gtacgcgata ttgaagcact
      841 ggatgaacta ctggcaacgc tgaccgatga taaaccgcga gtcattgcac tgcagccgat
      901 tagccaaaag gatgatgcca cacgattgtg cattgaaacc tgtattgcgc gtaattggcg
      961 tttgtcgatg caaacacata aatatttaaa tattgcctga ttacacattt ataagcgtt
//
LOCUS       DAJTSI010000368          990 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.378, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000368 DAJTSI010000000
VERSION     DAJTSI010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.378"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            82..>990
                     /locus_tag="OO327_004323"
     CDS             82..>990
                     /locus_tag="OO327_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HCR6020603.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGF"
BASE COUNT          301 a          206 c          228 g          255 t
ORIGIN      
        1 tgaggaatcc ccagaaaacg agacaaacat agcattatgt gatctactga tttgtgccaa
       61 caattcaatg agatcactgc tatgcctgtt cataaagtat gtcagaattt cttccgggat
      121 gccttagcac catttcatca gtaccgacaa aatgccctga tggatgccac aatggcgtta
      181 atcaacggag catctctgac gcagaccagt atcggacgtt ttttgccggg taatgcacaa
      241 gttaagaata agatcaaacg tattgatcgc ctcatgggta atgaggcgct ccatcgcgat
      301 attccaatga ttttcaggaa tatcacttca atgctgacac ggcagttatc tttatgtgta
      361 atagccgtag actggagtgg ctatccctcc caggagcatc atgttcttcg tgccagcctt
      421 ctgtgtgacg ggcgttccat tccgttattg agtaaggttg ttccttctga aaaacaaaat
      481 aatccattga tacaacatga tttccttgat tcccttgctc aatccctgcc ccccgatgcc
      541 agggttatta ttgtcacgga tgctggattt cagagcgcat ggtttcacca tattacttct
      601 ctgggctggg attttattgg tcggatcaga aataacgttc agtattgcct cgataacgcg
      661 cctgaaagat ggttaaaagt gtcggatagc cctgaatgta aaacaccgga atatatgggg
      721 gctggaaggc tggttaaaga aaggaaaaaa agcatccggg ggcattttta cacctataag
      781 aaatcggcaa aagggcgaaa aaagaaacgt tcgaaaggtc agagtggttt aaacaaaaca
      841 gacaaagaac agagcaaatc agcaaaagaa gcctggttga tcttcagcag tacaaatgac
      901 ttcagagcgc gagaaatcat caagttatac agtcggcgaa tgcagataga gcaaaacttt
      961 cgtgatgaaa agaacgggcg cttcggattt
//
LOCUS       DAJTSI010000369          947 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.379, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000369 DAJTSI010000000
VERSION     DAJTSI010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.379"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(201..866)
                     /locus_tag="OO327_004324"
     CDS             complement(201..866)
                     /locus_tag="OO327_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR6020604.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASEIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          245 a          232 c          215 g          255 t
ORIGIN      
        1 gtaagaggct aacctacatg tgcaaagcat gaaattggcc tcagattaat gttaagcgtc
       61 ttgccggacg cgtaatgtta actggggctt ttctctatct gccttttggt gttcatgcct
      121 gaagcagata gcctcaagca cccgcagtca ttctacttac ctcgccaata tgaaatcaat
      181 cagaaaggtg ccccataaaa tcactccttc tcttctttac cgtagtggag ttgaccaatt
      241 ttgataagag ggcgtccctg agatttgcgg tgtagattgg tatcgcgcag agaatacaca
      301 cagccacaat attcctgctg atagaatttt tcgcgcttgc tgatttcaat catacgggac
      361 gagccgccct gcttgcgcca gttataatcc cagtacacca tgcccggata atgcgcgacg
      421 gctcgccgcc cacagtcgtt aacctgctgc atatttttcc agcgtgaaat gcccagtgaa
      481 ctgctgatca cactgaaacc attttcagcg gcgtacagcg ctgtccgctc aaaacgcatg
      541 tcaaaacaca tggtgcaacg gatccctcgt tcgggctccc attccattcc tttggctcgt
      601 tcaaaccagt tgtctgtgtc gtaatcagca tcaataaacg gcacgccgtg ttgttcagca
      661 aagcgaatat tctcatcctt acgaattaaa tactctttct gaggatgaat gttcgggttg
      721 tagaaaaaga tggtgtagtc gatttccgag gcctgaagcg cctccatcac ctcaccggaa
      781 catggagcac agcaagagtg cagtagtagt ttgtttgccc cgtttgggag ctccaatttg
      841 ggccgtttga aatcagcaac tgtcataaat atgtttattg aggtcataaa aatatcaaag
      901 agtgtagcat tagaacgggg ctatcggaaa cagatgtgtg actgctc
//
LOCUS       DAJTSI010000370          942 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.380, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000370 DAJTSI010000000
VERSION     DAJTSI010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.380"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..280
                     /locus_tag="OO327_004325"
     CDS             <1..280
                     /locus_tag="OO327_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6020605.1"
                     /translation="FKWKSQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRN
                     KHDERPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            277..627
                     /gene="tnpB"
                     /locus_tag="OO327_004326"
     CDS             277..627
                     /gene="tnpB"
                     /locus_tag="OO327_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6020606.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFTKRLAGDPGRESAPDASSVIHATGGDRVATSQTDR
                     TAWHPDITRDKTRE"
     gene            624..>942
                     /locus_tag="OO327_004327"
     CDS             624..>942
                     /locus_tag="OO327_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR6020607.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSEN"
BASE COUNT          257 a          238 c          261 g          186 t
ORIGIN      
        1 gtttaaatgg aaaagccagt acgaggacgg cttactgagc gatgatgaca tacaggaatg
       61 catgcctgtc ccggtggcac tgactgatac gccggagccg accagaccag ttacaaatcc
      121 cttctggcgt aacaagcatg atgagcgccc tgagggggct cccggaaacg tcccacggtg
      181 cgagctgcat cttaaatcag gtgtggtaaa actgtttgac cctctcactc cggaactgtt
      241 acgggcgcta atccgcgaaa tgaaaggggg tatccgatga taacgctgcc gaccggtacc
      301 aaaatctgga tcatcgctgg catcacagat atgcgttgtg gcttcaatgg cctggcttcg
      361 aaggtgcaga acacgctgaa agatgacccg ttctccgggc atatcttcgt cttccggggc
      421 cgcagtggca aaatggtgaa aatactgtgg gccgatcgtg acgggttatg cctgttcacc
      481 aaacgtctgg ccggtgaccc gggaagggaa agtgcacctg acgccagctc agttatccat
      541 gctactggag gggatcgcgt ggcaacatcc caaacggaca gaacggcctg gcatccggat
      601 ataacccgtg ataaaacaag ggaatgaaca atgaactccc cgatgatatt gagctgctta
      661 aagccatgtt gcgtaagcaa cagagtcggc ttcgacagta tgcctgtcag gtcgcgggct
      721 atgagcagga aattgaacgg ctgaaagcgc aactcgacag gttgcgtcgt atgttgttcg
      781 gccagagttc agagaaaaag cgtcataagc ttgaaaatca gatccgacag gcagaaaaac
      841 gactgtcgga actggaaaac cggctgaaca cagccagaaa tcttctggaa gatgcatcgt
      901 cagtcacaga ttcacctgac accagtcccc cgtcagaaaa cc
//
LOCUS       DAJTSI010000371          933 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.381, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000371 DAJTSI010000000
VERSION     DAJTSI010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.381"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..804
                     /gene="lpxM"
                     /locus_tag="OO327_004328"
                     /gene_synonym="msbB"
     CDS             <1..804
                     /gene="lpxM"
                     /locus_tag="OO327_004328"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6020608.1"
                     /translation="KIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATA
                     LQSIVMMAELAIRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLA
                     AQGEKMAAMFHQQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPD
                     QDHGPEYSEFADFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMD
                     ACIASADNKMIARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT          270 a          181 c          229 g          253 t
ORIGIN      
        1 aaaatagggc gttgggtggg cagattgagc agaaaagctc gtcgcagggc gacgattaat
       61 ttatcgcttt gtttcccgga aaagagtgat acagaacggg aaataattgt cgacaatatg
      121 tttgccacag cattgcaatc catagtgatg atggcagaac tggcaattcg tggtccggaa
      181 aagttccaga aacgagtgtt ctggaaaggc ctcgaaattc ttgaggaaat tcggcacaat
      241 aacagaaatg tgatttttct ggttccccat ggctggagcg tggatattcc tgcaatgttg
      301 ctggcagctc agggggaaaa aatggccgcc atgtttcatc agcaacgaaa tccagtgatt
      361 gattatgtct ggaattctgt acggcgtaaa ttcggggggc gcttacattc ccgggaggat
      421 gggataaaac catttattca gtcagtacgc cagggatact gggggtatta ccttccagat
      481 caggatcatg gtcctgaata cagtgaattt gctgattttt ttgcgaccta taaagcgaca
      541 ttaccaatta ttggacgtct gatgaacatc agtcaggcta tgattatacc gcttttcccg
      601 gtttatgatg aaaaaaaaca tttcctgact attgaagttc ggccaccaat ggatgcatgc
      661 attgccagcg cggacaataa aatgattgcc cgacaaatga acaaaacagt ggaaattttg
      721 gtggggtcac atccggaaca gtatatctgg gttttaaaat tgttaaaaac gcgcaaatca
      781 aacgaagcgg acccgtaccc ttgaaattat aaagtacagg tatttccact agttgtttct
      841 tacaggttac caatcgaaac acatccccct tccgtgtgtt gcccgtttgg cacggaggac
      901 tgtgtagatg cagttcgtta aatttaattt atg
//
LOCUS       DAJTSI010000372          932 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.382, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000372 DAJTSI010000000
VERSION     DAJTSI010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.382"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(55..243)
                     /locus_tag="OO327_004329"
     CDS             complement(55..243)
                     /locus_tag="OO327_004329"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020609.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
BASE COUNT          245 a          181 c          216 g          290 t
ORIGIN      
        1 ccgcgctgat ggttcttatc cgttttattg aatcggagaa caaactgtga gctatcagcc
       61 tggtggtctg gttttgcctg atttaagccg ccgtgggtat ttgtccaaga atgcttcaag
      121 ctccaatcgt tttggcctgg tgaatggaga tgggttaagc aaagactctc gttgctggtg
      181 tactttttcg cgctggcgct caagcatgga taatgcgatt gcctgtagtt cttcgttagt
      241 cattgtctgc acctggaaag caatttaccg gagtacctca cactacggca aaaatgtttt
      301 attgttgcaa cttgcgtaat tattcaggtc agtttggaac gcactgtata aacataaata
      361 acctgatact gttgtgtgtt ttcaaagaaa acacaggtat ttttcacata aaccttcccc
      421 ttttcatatt cgaccactta atcaaaaaac ggttttatac ttgttgcccc cactggggtt
      481 aaatgtgaga catgtcgcac tttgtgccat gtgtcctcgc tgattttagg cgcggttcat
      541 tcattctttc tgttggcata aagtatggta tcggttgggc gaatgaaagg gactcgggtc
      601 atggtcagac aatacctgag gcgttaattc gttgaaagag attgtgaatg ggggaataac
      661 tgagtcttaa ctgctgtgcg atagcccgga cagatagttg ctcgtcgaat ctgagacgta
      721 atgattctgg caaataagtg cgaaagccgg tgatatctag tgttgtttga gagttcatcg
      781 ttcgcgtcca ttaatacaga tggacgcgat tgttgccaga caggataatt ttcacaagac
      841 atcgcagata gagtacttac gataattttt tattttatac aacagatgcc cacgaaaaga
      901 attattttca atagacacct gcattacaca aa
//
LOCUS       DAJTSI010000373          875 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.383, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000373 DAJTSI010000000
VERSION     DAJTSI010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.383"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>875
                     /locus_tag="OO327_004330"
     CDS             <1..>875
                     /locus_tag="OO327_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6020610.1"
                     /translation="SLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLA
                     MRRIADLHAGEAKTDARNAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAA
                     QTTQASNRIRGLLTQIHPAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQL
                     CKLAPRLGKRLAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVE
                     QRVLAHPLYPVLTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSI
                     RGEHPSRRGNKALKR"
BASE COUNT          178 a          291 c          230 g          176 t
ORIGIN      
        1 ggtcgctaat atctgacctg aaacaacatg gtcagatact gctggttgtt gatcagccag
       61 ctaccatcgg tgcgttacct gtcgccgttg cccgctcaga aggagtcctt gtcggatacc
      121 tccctggact ggccatgcgc cgcatagccg acttacacgc cggtgaagct aaaactgatg
      181 ctcgtaacgc tgccatcatt gccgaagctg cccgtaccct gcctcacgcg ctacgcacgc
      241 tgaaactggc tgacgagcaa atcgccgaac tctccatgct ctgcggcttc gatgatgatc
      301 ttgccgcaca gacaacgcag gccagcaacc gtatccgcgg ccttctgacc cagatacatc
      361 cggcaccgga gcgcgttctc ggtccgagac ttgagcaccc ggcggtactc gatcttctcc
      421 agcgatatcc ctcaccagaa aaactcgctt cgctgggtga gaagaagctg gcagcccagc
      481 tctgcaaact tgcgcctcgt ctgggtaaac gccttgcagc agacatagct caggcactgg
      541 ccgaacaaac cgtcgtcgtt cccggcacga atgccgctgc cgtagtactg ccacgtctgg
      601 cactccagct catcacgctg cgtaagcaaa gagacgaggt ggcgcttgag gtagaacagc
      661 gagttcttgc tcaccctctt tacccggtcc tgaccagtat gcccggagtc ggtgtcagga
      721 ccgcagccag actcctcacc gaggtcgcct gccgcgcctt cgcctctgcc gcacatctcg
      781 ctgcttatgc tggccttgcg ccggtaactc ggcgatccgg ctcgtcaata cgcggtgagc
      841 atccctcgcg acggggtaat aaagctctca aacgg
//
LOCUS       DAJTSI010000374          872 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.384, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000374 DAJTSI010000000
VERSION     DAJTSI010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.384"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..88)
                     /locus_tag="OO327_004331"
     CDS             complement(<1..88)
                     /locus_tag="OO327_004331"
                     /inference="COORDINATES: protein motif:HMM:NF025073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6020611.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
     gene            300..866
                     /locus_tag="OO327_004332"
     CDS             300..866
                     /locus_tag="OO327_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6020612.1"
                     /translation="MPPEIMRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTY
                     PSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELL
                     EDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRK
                     KILSKYGIYEQEGLVAPSQEPQDVKKAV"
BASE COUNT          285 a          176 c          179 g          232 t
ORIGIN      
        1 ctctggataa ttgagatagt gtcttcccct tcatacgaac agcagccttt atctgctctg
       61 cgtgccagtc ttggtttttt gctgacattc tgcgatcctt actctcttaa cgttttcggt
      121 aactgatacc ggctaccgct atcgggaaaa acgatagcgc agaaaaatgc tcaattcaaa
      181 agctatgcgc atttttattt taaccagtgc aaatcaaaga attctgtaaa attctttata
      241 taacagagag attgggcatg acaggtgaaa aagaaagcaa aaatgcagag cgcattttta
      301 tgcccccgga aataatgcgt ttcaaggaac gccttgaaga ggcaatgaat ggagaatcaa
      361 gccgggcgtt tgcagctaag tgcggacttt ctgatggggt tatcagaaac tacctgtctg
      421 gcaagacata cccgtcttta gatcgattag cacaaatagc ctacgcaaca gggcgtccaa
      481 tagagtggtt tatccaaagt cagagcatgc caatggttga acataaaaat aatcaagaga
      541 ataaaaattc tcaacctgac cgcagcacat tgaaaactaa actgatcgca attgtcgaat
      601 tgttagagga caaggaactg cattctgcga ttgagttgtt cagaaagaaa ggatttgaag
      661 ctcttatgcc tgagattttt tcttccgatg attccatcca ggctgatttg caaggtattt
      721 cgcagcaaac cttacaaaca gccaaaatgc ttgaatcatt accaacagat actcgtaaaa
      781 agattttgtc aaagtatggt atttatgaac aggaaggtct tgtagctcct tcacaagaac
      841 cacaagatgt aaaaaaagcc gtataacaac ca
//
LOCUS       DAJTSI010000375          870 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.385, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000375 DAJTSI010000000
VERSION     DAJTSI010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.385"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>870
                     /locus_tag="OO327_004333"
     CDS             <1..>870
                     /locus_tag="OO327_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR6020613.1"
                     /translation="LSSAAVKTAGVLISFQAVLSAYQRVMEVGLQRASAERSIDFVFS
                     KDANQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNMIGYSRLM
                     GRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVFAKATGKTEQELFDAM
                     KKGALKSADTLQKVTQELNKQITAKGGWKAISESTQAQLGNLKNSWNTTLDSIFRGSE
                     NGLQDFTRSLTNLLNALGGSGKSLGESLGSLMTSMSHGVDSLTDISYRVRAFFDEVTL
                     AYRPLNDTQRKIV"
BASE COUNT          263 a          153 c          209 g          245 t
ORIGIN      
        1 tttaagttca gcagcagtta aaacagcagg tgtacttatc agctttcagg ctgtactatc
       61 cgcttatcag cgagtaatgg aagttggcct tcaacgtgca agtgctgaac gttcaattga
      121 tttcgtattt tctaaagatg caaatcaagt aaaagatttc acaaagtcat tagctcaaac
      181 aacgggttta gatatcgctg aactgcaatc acagtttgct ggtttcggtg cttcagctag
      241 agagtctcta ggcattcaag gttctgaaga actttttaga aatatgattg gctattctcg
      301 tctgatgggg cgaagtgaag aagagattaa gcgagcactg acagcattat cacagatggc
      361 tggtaagggt cagattatgg ctgaagagct taaggggcaa ctagctgaag ctgttcctgg
      421 tatggttcag gtattcgcta aagcaaccgg aaagactgaa caggaattat ttgatgctat
      481 gaagaagggg gctttaaaat ctgccgatac tcttcagaag gtcactcagg aactgaataa
      541 acaaatcacc gctaaaggtg gctggaaagc tatttcagaa agtacacagg cacaactagg
      601 gaacttgaaa aactcttgga ataccacgct tgatagtatc ttcagaggtt ctgaaaatgg
      661 tctgcaagat tttacaagaa gcttaaccaa tcttttaaac gctcttggtg gaagtggtaa
      721 gagtcttggt gaatctcttg gtagtctcat gacttctatg tcacatggtg tcgattcatt
      781 gaccgatatt agttacaggg taagggcgtt ctttgatgag gtgacattag cttaccgtcc
      841 actaaatgac actcagagga aaattgttga
//
LOCUS       DAJTSI010000376          852 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.386, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000376 DAJTSI010000000
VERSION     DAJTSI010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.386"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..750)
                     /locus_tag="OO327_004334"
     CDS             complement(<1..750)
                     /locus_tag="OO327_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6020614.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFFLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHT"
BASE COUNT          248 a          149 c          164 g          291 t
ORIGIN      
        1 ggtatggcca gacaatggat tacctgcaac atttacgaag gcattctgag ctaatctcag
       61 gacactttcc ggaagtgttg tcagattatt attcattagc acagccctgt tcatactaaa
      121 aggtaattcc ggtagttgtt ctataaaatt attagccaga gccagccctt caagcttatc
      181 tggaaagggg gggatgctgt ctaatctgtt tccagaaact atcagtaatt ttagctccgg
      241 tggcaatagt ggcagaatat gcaatcggtt attatggatc cgtaaatgag ttagagtttc
      301 aggaagatat ggtaattctt ttatcttgtt atcacccaca ttaagctctt ctaacgtctc
      361 gggtaaggca ggtaagcttt ccagttgatt aaattgagca tgtattacct taagcattgg
      421 tgggaaatca gggagatatg ttaataggtt ttttcttatt tccagtgttg ttatctgagg
      481 cggtaaatca ggtaatgttt ttaaatctaa ctcagataga tttaattccg ccccttgatt
      541 ttgcaaacat atttttagcc tattgaaggc gatattgcgt tgctcgcctg gggtaccgtt
      601 cttttcccat tcactccaga cttttaaata atattcatta caagatggac tcaaactcac
      661 aggcaaaaaa aatagagtta acgagtgtat gttgtttatt tgatgcataa taccagaacc
      721 gattacctgt atattcgttg atttaatcat tataaccaga atttcttatc aacgataaga
      781 actctagtga aatacaatat aacttattaa gtttaataca gtttgctttg tactctttct
      841 ccaggtattg ct
//
LOCUS       DAJTSI010000377          849 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.387, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000377 DAJTSI010000000
VERSION     DAJTSI010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.387"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..561
                     /gene="istA"
                     /locus_tag="OO327_004335"
                     /pseudo
     CDS             1..561
                     /gene="istA"
                     /locus_tag="OO327_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(587..>849)
                     /locus_tag="OO327_004336"
     CDS             complement(587..>849)
                     /locus_tag="OO327_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
                     /protein_id="HCR6020615.1"
                     /translation="STGRILVLSLLATLSTIVMWLLGYHAENKGLHLKYQANSIKSRR
                     VISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRNMVLVY"
BASE COUNT          202 a          217 c          208 g          222 t
ORIGIN      
        1 gcaaaatctg agccgccaaa atatacgccg cgacctgctg ttgcttcact cctggatgaa
       61 taccgggatt atattcgtca acgcatcgcc gatgctcatc cttacaaaat cccggcaacg
      121 gtaatcgctc gcgagatcag agaccaggga tatcgtggcg gaatgaccat tctcagggca
      181 ttcattcgtt ctctctcggt tcctcaggag caggagcctg ccgttcggtt cgaaactgaa
      241 cccggacgac agatgcaggt tgactggggc actatgcgta atggtcgctc accgcttcac
      301 gtgttcgttg ctgttcccgg atacagccga atgttgtaca tcgaattcac tgacaatatg
      361 cgttatgaca cgctggagac ctgccatcgt aatgcgttcc gcttctttgg tggtgtgtcg
      421 cgcgaagtgt tgtatgacaa tatgaaaact gtggttctgc aacgtgacgc atatcagacc
      481 ggtcagcacc ggttccatcc ttcgctgtgg cagttcggca aggagatggg cttctctccc
      541 cgactgtgtc gccccttcag gctgagggat ccccacaaaa ttactgctaa taaaccagca
      601 ccatatttcg gtaggttctg gacagatggt ttagaactgt gctcagtact gttcgtctta
      661 aaataagtgg cgagtgtcga agaacattct tcgctaacgt cagatatgag ataacccgcc
      721 gggatttaat gctgttagcc tgatatttca ggtgtaaccc tttattttca gcgtgataac
      781 cgagtaacca cattacgata gtgctcaacg ttgccagcag actcagaacc agaattcttc
      841 ctgttgaac
//
LOCUS       DAJTSI010000378          840 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.388, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000378 DAJTSI010000000
VERSION     DAJTSI010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.388"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..654)
                     /gene="dkgB"
                     /locus_tag="OO327_004337"
     CDS             complement(<1..654)
                     /gene="dkgB"
                     /locus_tag="OO327_004337"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCR6020616.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     "
BASE COUNT          226 a          190 c          190 g          234 t
ORIGIN      
        1 tactgagtaa ccttccccca tagcccacgc cagaatcact tgtgccggaa tcgcattgtg
       61 tttagctgca atacgagcaa taacctcatc tttcagtgcc ttaccataag ccagcgtcat
      121 ataggaagtg atatgaatac cgtgctgttt agcccaggca accactttac ggttttgcag
      181 ataaggagag agttcaatct ggttggtagc gatgttttca gcgccaacag cagcaatcac
      241 tttttccatc aatgggatcg tgaagttgga aataccgatc tcacgcgtca gcccttgttt
      301 tttggcttcc agcagcgcct gcataaactc ttcaacagag acttcatcgt ttggtgacgg
      361 ccagtggatt agcgtcagat caacataatc ggtacgcaat ttttgcaggc tctctttcag
      421 actcgggatc aatttgtctt tgctgagatt ttcaatccag attttagtgg tgatgtagag
      481 ttcatgacgt ggcacgccac tttctgcaat cgcctgacct actgcggctt cgttatcata
      541 gatttgtgcg gtatcaattg cgcgataacc aagttcaagc gccgttttca cagatgaaat
      601 aacaacgtcg tctttcagac ggaaagtacc taaaccaaat gcagggatag ccataatatg
      661 cctcttttaa tgctattttc gttaactgag gtgattatgg gcttcagact gttgcgggaa
      721 aagagcgtaa aacgcagagg atttttgcga ttctagcaat aatagatatc cagacgcaaa
      781 aaaacatcga gcgttaactc gaggcttctt aataagtggc ggaacggacg ggactcgaac
//
LOCUS       DAJTSI010000379          831 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.389, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000379 DAJTSI010000000
VERSION     DAJTSI010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.389"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..326
                     /locus_tag="OO327_004338"
     CDS             <1..326
                     /locus_tag="OO327_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR6020617.1"
                     /translation="SETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGL
                     LLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVIL
                     KITYN"
     gene            complement(369..569)
                     /gene="glgS"
                     /locus_tag="OO327_004339"
     CDS             complement(369..569)
                     /gene="glgS"
                     /locus_tag="OO327_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HCR6020618.1"
                     /translation="MEHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          253 a          184 c          153 g          241 t
ORIGIN      
        1 ttagtgagac gtttagtgta gtcatgacaa aagatcctgg tgcggcctgt actgagcaat
       61 tcaatatttt agggagcttt ttcactacgg atattttgag tgattatagc catctggata
      121 tgggtaatgg actgctattg aagatatttc ataaagatgg aacagcaacg gaatttaacc
      181 gcttctcaca atttgcttct ttttcatcgt ctagtgcacc ttcggtcacc gcaccattca
      241 gggcagaact tagtgcgaac ccggcagaga ctgttgttga gggaccgttt agtaaagacg
      301 taatcctcaa aatcacctat aactagcatc taatacaaac actaaaacgg gccatcaggc
      361 ccgttatatc agtgctctaa ctccagctct cttgcctgca tcatctgttg gcaataccaa
      421 gcataacgtg cgcaaaacca agttctatgt tcttcatcca tgttaccagt aacagccaga
      481 atatcgaccg ggcgaccttc tgcgtgcatt ctggcaaaac tacgcgccag gaaatcgaaa
      541 ttattcaaag aattaagact atgctccata agcacatcct ccggttatcg catacctttt
      601 aaggccatat gctttttctg atttcagtat tccccaatca gtgtagtgcc tttaaacata
      661 acgtgcgtaa atatattgtc cccgatcaca ctttttagtg aaaaataaaa aaccatatta
      721 aaatactttc tcttccagaa cttctctaac atgtgttatt attaacaaag acaaatgtaa
      781 gcagggaaac tattccaagg taaatattgc gtcactaaat ggacattgga g
//
LOCUS       DAJTSI010000380          829 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.390, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000380 DAJTSI010000000
VERSION     DAJTSI010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.390"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..786
                     /gene="icsP"
                     /locus_tag="OO327_004340"
     CDS             <1..786
                     /gene="icsP"
                     /locus_tag="OO327_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCR6020619.1"
                     /translation="GGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQK
                     ASMVDKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQE
                     SRYSFNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEF
                     GAELKYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRN
                     YISNKKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
BASE COUNT          279 a          133 c          178 g          239 t
ORIGIN      
        1 ggggggagaa aaattagtca actggactgg aaatacagta atgcgactat tgttagaggt
       61 ggcatcgatt ggaagctaat tccaaaagtg tctttcggag tttccggttg gactacttta
      121 ggtaaccaga aagcaagcat ggttgataaa gactggaaca attccaatac ccctcaggta
      181 tggaccgacc agagctggca tcccaatacg catctccgtg atgctaacga attcgagctg
      241 aatcttaaag gttggttatt aaataatttg gattatcgac ttggactaat agcaggttac
      301 caagagagtc gttacagttt taatgcaatg ggagggagtt atatttatag tgaaaacggg
      361 gggagcagga ataaaaaagg ggcacatcct agtggtgaaa gaacaatagg ttacaaacag
      421 ctcttcaaaa taccttatat tggattaact gctaattacc gccatgagaa ttttgagttt
      481 ggagcagaac tgaaatatag tggttgggtt ctttcatctg atacagataa acactatcag
      541 actgagacaa tttttaaaga tgaaataaaa aaccaaaatt actgctctgt tgctgcgaat
      601 attggatact atgtcacccc cagtgcaaaa ttttatatag aaggctccag aaattacatt
      661 tctaataaaa aaggtgatac atctctttat gagcaaagta ccaatatatc tggcaccatt
      721 aaaaatagtg caagtattga atatattggt tttctcactt ccgcaggtat aaagtatatt
      781 ttttgaattg gttcaccgag ttgattacgt tttcatggct aaaaattgc
//
LOCUS       DAJTSI010000381          782 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.391, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000381 DAJTSI010000000
VERSION     DAJTSI010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.391"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(570..>782)
                     /locus_tag="OO327_004341"
     CDS             complement(570..>782)
                     /locus_tag="OO327_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR6020620.1"
                     /translation="WLSGDSGGQSTGYKDSDRLNYIEIGTWYYFNKNMNIYTAYQINL
                     LDKSDYVLAHGLNTDDQLAVGIVYQF"
BASE COUNT          213 a          131 c          201 g          237 t
ORIGIN      
        1 ttagggtagt cactatattt gcgactgagc tggtcgctat ttttaaccag gctgaaaaat
       61 tgagtcaata tatcgaaagc ggtagttggc gggcataagt atgtaatgat gatgctgcac
      121 tggtgttgaa tattattcac gccaaacttt cctcattgag caatgtcgtc gcaagtggcc
      181 tcaatgattt tagaccatta tcagaggtcc ggagcggcgg gaagagattt attgctgtgg
      241 aataattgtg gtactgatga ttatttatat cccggattgg tagggaaaag cttttttcat
      301 gcactatttt tgcaaatatt tctatatggg aagtggtttt gttacagatt aaatttataa
      361 agaaatgcat ttggggtgtt gggttgaaga gtagctggta gatgttattg tgccagcagg
      421 ggcaggggaa tgtgccgtat cgatatgctt tggcaattgc gtgtgccgca gatggagatg
      481 aaatgccaat aaaaaagcct gagttcgatg agggacgaac tcaggctggt gttatgtttt
      541 acagtaacac gcctcggcgc gttggctgat tagaactggt agacgatacc aactgccagt
      601 tggtcgtcgg tgttcagtcc atgagcgaga acataatcac tcttatcaag caggttgatc
      661 tgataagcgg tatatatgtt catattctta ttgaagtaat accaggtacc gatttcaata
      721 tagttcagac gatcagaatc tttgtaacct gtagactggc cccctgaatc tccagacaac
      781 ca
//
LOCUS       DAJTSI010000382          765 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.392, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000382 DAJTSI010000000
VERSION     DAJTSI010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.392"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            8..765
                     /locus_tag="OO327_004342"
                     /pseudo
     CDS             8..765
                     /locus_tag="OO327_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          223 a          162 c          189 g          191 t
ORIGIN      
        1 ccctgatatg tcaaagccaa aatacccttt tgaaaagcgc cttgaagtcg tgaatcacta
       61 cttcactact gatgatggtt acaggatcat ctcggcacgt tttggtgtcc cccgaaccca
      121 ggtcaggaca tgggttgccc tctatgaaaa acatggagaa aaaggcttaa ttcccaaacc
      181 taaaggtgtt agtgctgatc cagagttgcg tattaaggtc gtgaaagctg tgattgagca
      241 gcacatgtcc cttaatcagg ctgctgctca ctttatgctt gctggtagtg gttctgtagc
      301 caggtggctg aaggtttatg aagagcgcgg agaagctggt ttacgcgcgc tcaagattgg
      361 caccaaaaga aacattgcaa tatcagttga tccagaaaaa gcggcatcag cattggagct
      421 gtcaaaagac cgacgcattg aggatcttga aaggcaagtt cgatttcttg aaacgcggct
      481 tgtgtatcta aaaaagctga aagccttagc tcatcccacg aaaaagtgaa agtactcaac
      541 gagctaaggc agttttatcc tcttgatgag cttctcaggg ctgcggagat accgcgcagt
      601 acgttttatt accatctaaa ggctctcagc aagcctgaca agtatgcgga cgttaaaaag
      661 cgtattggtg agatttatca tgagaataga ggccgatacg gataccgtag ggtaacgctg
      721 tctcttcatc gagaagggaa acagattaac cataaagctg ttcac
//
LOCUS       DAJTSI010000383          763 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.393, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000383 DAJTSI010000000
VERSION     DAJTSI010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.393"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            459..>763
                     /locus_tag="OO327_004343"
     CDS             459..>763
                     /locus_tag="OO327_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6020621.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCV"
BASE COUNT          259 a          152 c          127 g          225 t
ORIGIN      
        1 tgttgaaaca aatgacggta gagaatgtta acactgccgt taaacaatat ctttctcatc
       61 cggtaaatac ttataccgga gtattattgc caaaataata acagccccgt caacacatcg
      121 ttggcgggga ttttagcaat attcgctatt tttatgtaat aattttataa atgcgttcaa
      181 aataataacc aagtactaat agtgatattt taatacctga tttttacgtg ataattcagg
      241 aggcacagaa agcgcataaa aataacaaca taaaacacct tgccaccacc caagaattct
      301 ctattacatt gtttttaatg aaaaaatatc attccaacaa tatctcacga taaataacat
      361 taggattttg ttatttaaac acgagtcctt tgcacttgct tactttatcg ataaatccta
      421 cttttttaat gcgatccaat cattttaagg agtttaaaat ggataagaag caagtaacgg
      481 atttaaggtc ggaactactc gattcacgtt ttggtgcgaa gtctatttcc actatcgcag
      541 aatcaaaacg ttttccgctg cacgaaatgc gcgacgatgt cgcatttcag attatcaatg
      601 atgaattata tcttgatggc aacgctcgtc agaacctggc cactttctgc cagacctggg
      661 acgacgaaaa cgtccacaag ttgatggatt tatccattaa caaaaactgg atcgacaaag
      721 aagaatatcc gcaatccgca gccatcgacc tgcgttgcgt aaa
//
LOCUS       DAJTSI010000384          760 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.394, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000384 DAJTSI010000000
VERSION     DAJTSI010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.394"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>760
                     /locus_tag="OO327_004344"
     CDS             <1..>760
                     /locus_tag="OO327_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR6020622.1"
                     /translation="ERILTDRENGEKGDNSQDTSYARVGVKGETQINPEMTGYGQFEL
                     DLEASNRHNPDQTRLAYAGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGGDSWAGT
                     DLFMTNRTNGVATYRNTDFFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTID
                     GFSFAGAYANSDRTDWQSGDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGD
                     VVNKTQNFEAVLQYQFDFGLRPSIGYSYSKALDVA"
BASE COUNT          211 a          155 c          184 g          210 t
ORIGIN      
        1 tgagcgcatt ctgaccgacc gtgaaaatgg tgaaaaaggc gataattctc aagatacctc
       61 ttatgcccgt gtaggcgtga aaggtgaaac tcagatcaac cctgaaatga ctggttacgg
      121 tcagtttgaa ctagacctgg aagcatcaaa cagacacaac cctgaccaaa ctcgtttggc
      181 atatgctggt ttgagttaca aagattttgg ttcattcgac tacagtcgta atgtcggtgt
      241 cgcgtatgat gcagaagcat ttactgatat gtttgttgag tggggcggtg actcatgggc
      301 tggtactgac ctgttcatga ccaaccgtac caacggtgtt gcaacctatc gtaacacaga
      361 tttctttggc atggttgaag gtctaaattt tgctctgcaa tatcagggta aaaatgaagg
      421 tacaggcaac tacaaagcaa atggcgatgg ccatggttta tctgcaactt acactattga
      481 cggtttctca tttgctggtg cttatgcaaa ctctgaccgt actgattggc agtctggtga
      541 tggtaaaggc gaaagagccg aagtttgggc gttgtcaaca aaatatgacg ctaacaatgt
      601 ttatgctgct gtgatgtacg gtgaatctca caacatgaat tccgacgacg gtgatgttgt
      661 gaacaaaaca caaaacttcg aagctgttct gcaatatcag ttcgactttg gtctgcgtcc
      721 atccatcggt tactcctatt ctaaagcact ggacgtagca
//
LOCUS       DAJTSI010000385          751 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.395, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000385 DAJTSI010000000
VERSION     DAJTSI010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.395"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            426..>751
                     /locus_tag="OO327_004345"
     CDS             426..>751
                     /locus_tag="OO327_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6020623.1"
                     /translation="MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPD"
BASE COUNT          274 a          139 c          120 g          218 t
ORIGIN      
        1 gatgaaaaac aaagccaacg gtattctgat ttctgaagtt aatttcagac tcgtttaagc
       61 tccattttca aaaaaaatat atcagggttt gttactaata acttctgaaa acagaaaaaa
      121 atagagcagt aattctattt cagagaggag taactccatc tatcctcgat acgtactgaa
      181 cgttatcgtg atatattcat catgctttct tcaccattat tcgagtatag ggagagacgc
      241 aatgacctta aatacctttt tatggctgaa ttaccattct caggaataac agcagactcc
      301 ttcccataga cggcttaggg ggaaagcaat acccggagaa agggtacaaa gcaaactgtt
      361 ttaaaattaa taagccatat tctatttaac tagaattctt atcgttgata agaattctag
      421 ttataatgat taaatcaacc aatatacagg caatcggttc tggcattatg catcaaataa
      481 acaatgtata ctcgttaact ccattatctt tacctatgga actgactcca tcttgtaatg
      541 aattttattt aaaaacctgg agcgaatggg aaaagaacgg taccccaggc gagcaacgca
      601 atatcgcctt caataggctg aaaatatgtt tacaaaatca agaggcagaa ttaaatttat
      661 ctgagttaga tttaaaaaca ttaccagatt taccgcctca gataacaaca ctggaaataa
      721 gaaaaaacct attaacacat ctccctgatt t
//
LOCUS       DAJTSI010000386          727 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.396, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000386 DAJTSI010000000
VERSION     DAJTSI010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.396"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..617
                     /locus_tag="OO327_004346"
                     /pseudo
     CDS             1..617
                     /locus_tag="OO327_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          191 a          161 c          248 g          127 t
ORIGIN      
        1 atgcggcaac ggcttctgaa agcgtggtca gaggggctgg cgatgccgga gtcgttgtca
       61 catatcacga cggaatcaca gaggagaagc ctggtgctga aagccggagg aaaatactgg
      121 catgtgtaca tgtcgaaaaa aacggccggc gggaggaata cggcgcgcta cctgggtcgt
      181 tatctgaaga agccgccgat agcggcctcc cggctggcac attacaacgg agggggagcc
      241 tgagcttccg ttacctggac cacaaaacgg gagaaacggc gacggaaacg ctgacacagc
      301 gtgagctggt cgcgaggctg aaacagcaca tcccggagaa gttttttaag atggtgaggt
      361 acttcgggtt ccttgccaac cgggtgtgtg gagagaagct gccgcaggtg taccgtgctc
      421 tggggatgga taaaccggaa ccagtggcga aagtgtgcta tgcacaaatg gtgaaacagt
      481 tcctgagtcg tgacccgttc gaatgcgtgc tgtgtggcgg ccggatggta taccgccggg
      541 ccatcgcggg actgaatgtg gaggggctga agaaaaacgc gcgggatatc agtctgctga
      601 ggtatatgcc ggcctgacac aggctaagtg tgcccgggaa tcggtgcaag tataaaaaac
      661 agcctgttgc ggtataagaa tcgaccatgc agagagagaa aacaccggag tggagggaaa
      721 agcagaa
//
LOCUS       DAJTSI010000387          717 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.397, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000387 DAJTSI010000000
VERSION     DAJTSI010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.397"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(54..717)
                     /locus_tag="OO327_004347"
                     /pseudo
     CDS             complement(54..717)
                     /locus_tag="OO327_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
BASE COUNT          207 a          147 c          138 g          225 t
ORIGIN      
        1 cagtgggtat atctgttcag aatatttcac gtagcacacg gatatataag aaatcattct
       61 tcctctggat agcttgaact taagagcata tcctgcgtga attggtcatt agctgattga
      121 ttgaagctaa agaaagatgg tgcatccggt ctaacagttg cttcaccagt gatattaact
      181 gataaaggct tcttagtttc tgctgttaaa gcgtctacct ttcttcctaa ctctgataca
      241 gccatagcta attgagcatt gctattaacc atgtcacctg tgatagctga tggagttgat
      301 tgtgtgttat ttacgaagtt attcatcggt tcttggaagt tagcaatttt attagttgca
      361 ccattaaacc agtcagcgat agaactattg gcttagatga atcacctttg tattcagggt
      421 tgaagtttgt ttctgtgcca atacctgatt gatgtaagtt cggattctta gctgcttcag
      481 ggtttaaatt atcaagagca aaatttacca actcagcata aaggacaact ctaccgactt
      541 tatttagcgc accacctgaa gaaccagcct tgccagcttt agctttatta acagcgtcac
      601 cgaatgaagt aacagcgttt gcaaggttta acaagcctcc tgctacattt gcagcctttt
      661 gagcagtaac agccacagca agcatcttca gaccatcaat taacccatca gataaac
//
LOCUS       DAJTSI010000388          704 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.398, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000388 DAJTSI010000000
VERSION     DAJTSI010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.398"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..443)
                     /locus_tag="OO327_004348"
     CDS             complement(<1..443)
                     /locus_tag="OO327_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR6020624.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPD"
BASE COUNT          214 a          172 c          131 g          187 t
ORIGIN      
        1 tcatctggat aatcggcatg agcaataaca tgcttgccgg tgcgactgtg tacttcaaga
       61 ataaagggca aatgctgaca tttttccagc gccgttttcg cttgtttctg ttgattatct
      121 gccagcgcaa taaaccagtc gccgccattc atcaaccaca aagacatctg ctggaatgcc
      181 agcgcatcca tcgccatctg ttcatgattg cctcttaccg cacgaaccca atgttgttcc
      241 agtaactgca gacaacgtaa actttgcggc ccacgatcga taacgtctcc cactgagata
      301 agtaaatctc gccacggatc aaaacgacaa tgccataatt tgcggcgcaa ctgctcaaga
      361 caaccgtgta tatcgccaga aagccagata tgtcgccatt gatgacccgc aattctctga
      421 taaacgggcg caggctgttt catcaatatt ttcctcccgc gctaaagatc acataatctt
      481 aacaagaatg ttaaaaaacg ctggactcag acagtagagt gtgtgttatg gttgactata
      541 aagtcagcga aggaaatgct tctggctttt aacagataaa aagagaccga acatgattcc
      601 tgttttcgtc aacaaacaat aaaacccttt aaaattaatg cgttgaataa atttacgttc
      661 atcttttaat ccctgacacg taccattgca tattaataca ttca
//
LOCUS       DAJTSI010000389          703 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.399, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000389 DAJTSI010000000
VERSION     DAJTSI010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.399"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            119..>703
                     /locus_tag="OO327_004349"
     CDS             119..>703
                     /locus_tag="OO327_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCR6020625.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPYCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITR"
BASE COUNT          145 a          192 c          210 g          156 t
ORIGIN      
        1 tcgcccgctg cacggagcgg gaatttgcga ccattcttgc cggtatcagc gccggcggta
       61 tgtactgccc ggagtgtggc acggttcact ggccggacgg agtcgccccg cccttctgat
      121 gctcccccgt tttgccgata tttttcagca gggaaaccgc tggcttaact ggctggagaa
      181 acaaccggaa ggggcagtcc gtccggtggt aattgaatca gtgacaaaaa tcatggcctg
      241 cgggaccaca ctgatgggct acacgcagtg gtgttgttca tcaccggact gttgccatac
      301 aaaaaaggtc tgcttccggt gtaaaagccg ctcctgcccg cactgcgggg tgaaggctgg
      361 cgcacagtgg atacagtatc tgctgagcct ggtcccctac tgcccgtggc agcatattgt
      421 gttcacactt ccctgccagt actggtccct ggtgttccac aaccggtggt tactggcaga
      481 gatgagccgc attgcagcgg atgtgatact ggaaatctgc catcaggcag atgtggagcc
      541 ggggatattc acggtgatcc acacatgggg gcgtgaccag cagtggcatc cgcatatcca
      601 tttatcgaca actgccggtg gtgtgacgtc gggccacacc tggaaaaatc ttcattttta
      661 cgcccgtaag gtgatgagca tgtggcgcta ccggataaca cgg
//
LOCUS       DAJTSI010000390          694 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.400, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000390 DAJTSI010000000
VERSION     DAJTSI010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.400"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            32..493
                     /locus_tag="OO327_004350"
     CDS             32..493
                     /locus_tag="OO327_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCR6020626.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(534..>694)
                     /locus_tag="OO327_004351"
     CDS             complement(534..>694)
                     /locus_tag="OO327_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR6020627.1"
                     /translation="LAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLA
                     ARQHVKKS"
BASE COUNT          204 a          152 c          159 g          179 t
ORIGIN      
        1 agtttcttga ttaaataaaa tggagtttta catgaaggct ttcaataagc tgttttccct
       61 cgttgttgca tctgttctgg ttttctctct tgctggctgc ggtgacaaag aagaatcgaa
      121 gaaattcagc gccaatctga acggcactga aattgccatt acctatgtct acaaaggtga
      181 caaggtgctt aagcaatctt ctgaaaccaa aattcaattt gcctccattg gtgcaaccac
      241 caaagaagat gctgccaaga cacttgagcc gttaagcgcc aaatacaaaa acatcgcggg
      301 tgttgaagaa aaatcaacct ataccgatac ctacgcgcag gaaaacgtga ctatcgatat
      361 ggaaaaagtg gattttaaag ccctgcaggg tatttcagga atcaacgttt ctgctgaaga
      421 tgccaaaaaa ggtatcacta tggcgcaaat ggaactggtg atgaaagccg ctggttttaa
      481 agaagtgaaa taatctgtcg gcggccatgt ttcgcatggc cgccataccc gtcttagctt
      541 ttcttcacat gctggcgcgc tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg
      601 cattcgcgga agtttttatg atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa
      661 acgcggaact gctgttcggt gaggatagcc agaa
//
LOCUS       DAJTSI010000391          689 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.401, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000391 DAJTSI010000000
VERSION     DAJTSI010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.401"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            346..639
                     /locus_tag="OO327_004352"
     CDS             346..639
                     /locus_tag="OO327_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR6020628.1"
                     /translation="MFCAIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
BASE COUNT          186 a          144 c          169 g          190 t
ORIGIN      
        1 tggcctttgc tgaactcaac attgacattc ttacccgcag caaagctcgt ggaggttagc
       61 agtgcaagca gcaatacagc cttactgatg ctcttgattt tcattggtag tctcctgttc
      121 agaatgagta cgatgaactg ttataatata acaatccctt acgggagaat tccagtggct
      181 tttctgcgtt cggtaaaatc ccggcgcggc attttttgcc ggggggatta cttataaagg
      241 ggcatggact caacaaaccg cagaaatcta cagctggctg gcttgtggcg cgggtttcat
      301 tgatgttagc ttatgccaaa acctggcatc ttaaagagta aaagtatgtt ttgtgcgatt
      361 tatcgaagca gcaagcgtga ccagacctat ttatatgtcg aaaaaaaaga cgatttttcg
      421 cgtgttcctg aggaactgat gaaaggtttt ggtcagcctc agttagcgat gattctgccg
      481 ttggatgggc ggaagaaact ggtcaatgct gatattgaaa aggttaaact ggcattaacc
      541 gagcaaggtt actatttgca gttaccgcca ccacccgaag atttgctgaa gcaacatctt
      601 tccgtcatgg ggcagaaaac agacgacact aacaaataac cgatatccgg cggtggcatt
      661 atctttgtcg gcgcgggttt tcatatcca
//
LOCUS       DAJTSI010000392          671 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.402, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000392 DAJTSI010000000
VERSION     DAJTSI010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.402"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..525
                     /locus_tag="OO327_004353"
                     /pseudo
     CDS             1..525
                     /locus_tag="OO327_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            589..>671
                     /locus_tag="OO327_004354"
     CDS             589..>671
                     /locus_tag="OO327_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6020629.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASV"
BASE COUNT          187 a          135 c          163 g          186 t
ORIGIN      
        1 tgtgctgatc gtgcggatga ttactaccat gaccgtctgg cgctgactgt tgataattca
       61 acgatcgacg acaactacga acactacaca tataacggca cttacaataa tgccgctgat
      121 actcatgttg tagacgttta caacattggt acggctatta ctctggatca ggaagttgat
      181 ctgtccatca ctaataactc tcacgttgcg ggtattacgt tgactcaggg ttatgagtgg
      241 gaagatattg acgacaacac tgtcagcact ggcgtaaaca gcagcgaagt gtttaataac
      301 accatcactg ttaaagattc taccgtaacc tctggttcat ggactgatga aggtactact
      361 ggttggttcg gtaatacagg taatgccagc gattatagtg gtaaatctaa ctttgtcacc
      421 gtagatactg acggagatgg tgttgctgat agcactattg caagctggga tgatgttgca
      481 ttagctgtag ttgcacatcc gaatgttgat aatgctatgc agacctgaat cgccacgggt
      541 ttaacagaca cctcagagtc atttaagatg gcttaaagag aggtgcccat gagcggtaag
      601 cgttatcccg aagagtttaa aactgaagca gtcaaacagg ttgttgatcg cggttattct
      661 gttgccagcg t
//
LOCUS       DAJTSI010000393          661 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.403, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000393 DAJTSI010000000
VERSION     DAJTSI010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.403"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>661
                     /locus_tag="OO327_004355"
     rRNA            <1..>661
                     /locus_tag="OO327_004355"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          169 a          153 c          215 g          124 t
ORIGIN      
        1 actactggaa acggtagcta ataccgcata acgtcgcaag accaaagagg gggaccttcg
       61 ggcctcttgc catcggatgt gcccagatgg gattagctag taggtggggt aacggctcac
      121 ctaggcgacg atccctagct ggtctgagag gatgaccagc cacactggaa ctgagacacg
      181 gtccagactc ctacgggagg cagcagtggg gaatattgca caatgggcgc aagcctgatg
      241 cagccatgcc gcgtgtatga agaaggcctt cgggttgtaa agtactttca gcggggagga
      301 agggagtaaa gttaatacct ttgctcattg acgttacccg cagaagaagc accggctaac
      361 tccgtgccag cagccgcggt aatacggagg gtgcaagcgt taatcggaat tactgggcgt
      421 aaagcgcacg caggcggttt gttaagtcag atgtgaaatc cccgggctca acctgggaac
      481 tgcatctgat actggcaagc ttgagtctcg tagagggggg tagaattcca ggtgtagcgg
      541 tgaaatgcgt agagatctgg aggaataccg gtggcgaagg cggccccctg gacgaagact
      601 gacgctcagg tgcgaaagcg tggggagcaa acaggattag ataccctggt agtccacgcc
      661 g
//
LOCUS       DAJTSI010000394          660 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.404, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000394 DAJTSI010000000
VERSION     DAJTSI010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.404"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(35..>660)
                     /locus_tag="OO327_004356"
     rRNA            complement(35..>660)
                     /locus_tag="OO327_004356"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          144 a          195 c          157 g          164 t
ORIGIN      
        1 ctgtgtgagc actacaaaga acgcttcttt aaggtaagga ggtgatccaa ccgcaggttc
       61 ccctacggtt accttgttac gacttcaccc cagtcatgaa tcacaaagtg gtaagcgccc
      121 tcccgaaggt taagctacct acttcttttg caacccactc ccatggtgtg acgggcggtg
      181 tgtacaaggc ccgggaacgt attcaccgtg gcattctgat ccacgattac tagcgattcc
      241 gacttcatgg agtcgagttg cagactccaa tccggactac gacgcacttt atgaggtccg
      301 cttgctctcg cgaggtcgct tctctttgta tgcgccattg tagcacgtgt gtagccctgg
      361 tcgtaagggc catgatgact tgacgtcatc cccaccttcc tccagtttat cactggcagt
      421 ctcctttgag ttcccggccg gaccgctggc aacaaaggat aagggttgcg ctcgttgcgg
      481 gacttaaccc aacatttcac aacacgagct gacgacagcc atgcagcacc tgtctcacgg
      541 ttcccgaagg cacattctca tctctgaaaa cttccgtgga tgtcaagacc aggtaaggtt
      601 cttcgcgttg catcgaatta aaccacatgc tccaccgctt gtgcgggccc ccgtcaattc
//
LOCUS       DAJTSI010000395          656 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.405, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000395 DAJTSI010000000
VERSION     DAJTSI010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.405"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(151..648)
                     /locus_tag="OO327_004357"
     CDS             complement(151..648)
                     /locus_tag="OO327_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR6020630.1"
                     /translation="MTTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGNDLKE
                     LRVALNYSQNPVSPKARSLILWTERGAARHGKMLETDQAWDVFEKLEDCYFSQRDPST
                     PVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDKLVE
                     RLMQL"
BASE COUNT          173 a          160 c          144 g          179 t
ORIGIN      
        1 gtttaacgcc ctgtgccatt tttcaggcac aaaaaaaccc gcataaagcg ggttctttca
       61 agtgtccatg tctgctattc gcctcgcgat acagctttgc gaagcttacc gtaattgaag
      121 cagtttatgt gcaaaaatgc aagaactttt ttaaagctgc atcaaccttt ccaccagttt
      181 atctctgcga acaacaaacc aaccattggc tctcgccagt tccagccatg actcaaggga
      241 aataacaata tcatcatccc gtaactgaat tgtggaaaca gtgacaccgc ctctctgata
      301 acagagaact cgcgtgtcgt aacttttctg gcatgaaact ggcgttgacg gatccctttg
      361 actgaaatag cagtcttcca gtttttcgaa cacatcccac gcctgatcgg tttcgagcat
      421 tttgccgtgg cgtgctgcgc ctcgttctgt ccagaggatg agggagcggg ctttgggtga
      481 aactggattt tgtgagtagt ttaaagcgac ccgcaattct ttaaggtcat taccaacaac
      541 tttgaaaaag tgtttccctt caacgaagcg tactttgttc tcatgatgat tctggcgaat
      601 acgcaccggc tcagtgccgt aaagctgcgc caaaagttcg gtggtaataa caggaa
//
LOCUS       DAJTSI010000396          654 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.406, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000396 DAJTSI010000000
VERSION     DAJTSI010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.406"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..219
                     /locus_tag="OO327_004358"
     CDS             1..219
                     /locus_tag="OO327_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR6020631.1"
                     /translation="MTGENRGRGRPRKTESTYADTRNDLIRSGLELLTQNGFLATGVD
                     AIVKNANVPKGSFYYYLKSKEDYAVNDG"
     gene            complement(270..>654)
                     /gene="istB"
                     /locus_tag="OO327_004359"
     CDS             complement(270..>654)
                     /gene="istB"
                     /locus_tag="OO327_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HCR6020632.1"
                     /translation="CSVDAYADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYL
                     PFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLDRILHHSHI
                     VQIKGESYRLRQKRKAGVIAEANPE"
BASE COUNT          166 a          153 c          163 g          172 t
ORIGIN      
        1 atgacaggcg aaaatcgtgg ccggggaagg ccgcgaaaga cagaaagtac ctatgcagat
       61 actcgtaatg atctgatccg cagtggcctt gaactgctga cgcagaatgg ttttcttgcg
      121 acgggagtcg atgccattgt taaaaatgcc aatgtcccta aaggctcatt ttattactac
      181 cttaagagta aagaggatta tgctgtcaac gacggatgaa aagtgatcca cttatatctc
      241 caccaacggc ccaatattga tccaccgttt tactcaggat tagcttctgc tataaccccg
      301 gcctttcgtt tctgtctgag tcgatagctt tctcctttga tttgaacgat atgtgagtgg
      361 tgtaagatac ggtccagcat cgctgaggtc agtgctgcat caccggcgaa cgtttgatcc
      421 cactgcccga acggcagatt ggatgtcagg atcattgcgc tcttttcgta acgtttagcg
      481 atgacctgga agaacagctt tgcttcttcc tgactgaacg gcagatagcc tatttcatca
      541 atgatgagca ggcggggggc cattactcca cgctgaagcg tcgttttata acggccctga
      601 cgttgtgccg tagataactg aagtaacaga tctgcgtaag cgtcaacgga gcac
//
LOCUS       DAJTSI010000397          650 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.407, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000397 DAJTSI010000000
VERSION     DAJTSI010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.407"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>650)
                     /locus_tag="OO327_004360"
     CDS             complement(<1..>650)
                     /locus_tag="OO327_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR6020633.1"
                     /translation="LERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGG
                     MRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLMT
                     ELYRHLGADTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEA
                     TGYGLVYFTEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSG"
BASE COUNT          160 a          187 c          161 g          142 t
ORIGIN      
        1 gccgctggag tctgacgcag tgatcacacg agcaccaaat tccatcgctt tttcgatagc
       61 gtactgggcg acgttgccgg agccagaaac ggaaacgctc atcccttcaa aacccatacc
      121 gtggcgtttt aacattgctt ctgtgaaata aaccagaccg tagccggtag cttccgggcg
      181 aataagacta ccgccaaatg aaaggccctt accggtgaag acgcaggcgg tattgttgga
      241 gagctttttc atcatccccg ccataaagcc gacttcacga ccaccaacgc cgatatcacc
      301 tgccggaacg tcggtatccg cgcccaggtg gcgatacagt tcagtcatca gcgtctggca
      361 aaaacgcatc actttacctt cgctttttcc tttcggatcg aaatcgctgc cgcctttacc
      421 accgcccatc ggcagggtag tcagggcatt tttgaaggtt tgttcaaagc cgaggaattt
      481 gagaatggaa aggttaactg acggatggaa gcgcataccg cctttgtacg ggccgatggc
      541 agagctgaac tgcacacgcc atgcacggtt gacctgtatc tggttgcgat catcaaccca
      601 taccacgcga aactggatca cgcgctccgg ttcaaccaga cgctccagta
//
LOCUS       DAJTSI010000398          641 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.408, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000398 DAJTSI010000000
VERSION     DAJTSI010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.408"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>641
                     /locus_tag="OO327_004361"
     CDS             <1..>641
                     /locus_tag="OO327_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6020634.1"
                     /translation="VSFYRTTSIDNVHNNYLSEWVEWTKNSISGENRETAFTRLQLCL
                     ENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALKELNASSNQLSALP
                     ELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMSLELLDAARNALEV
                     IPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQLSPRIMQTL"
BASE COUNT          205 a          132 c          118 g          186 t
ORIGIN      
        1 gtctcttttt acagaactac atcaatcgac aatgttcaca ataattatct ctccgaatgg
       61 gttgaatgga ctaaaaacag catttccgga gaaaacaggg aaactgcttt tacccggctc
      121 caattatgtc tggagaacag tgaaacatcg ttggacttat cttgtttagg tctcagatct
      181 ctaccacgat tgcctgacaa tcttgatgaa attaatgtaa gcaataacca actatcaatg
      241 ctccccgagc taccaagggc attgaaagag ctgaatgcaa gcagtaatca attatctgca
      301 cttcctgaat taccagtgtc gctggaatat ataaatgtga gtgataacca tttgttcgca
      361 cttcctgaat tacctgcgtc actagaatat attaatgtaa gtgacaatca cctgtctgta
      421 cttccgaggt taccaatgtc attggaatta cttgatgcag ccagaaatgc tttggaagta
      481 ataccagatt ttccagaaag agatgatcat attataagaa tattctggct taatcagaac
      541 cggatcacgg caattccgga aagcatactt ggcctcagtt ctgatagcgt tgtcaatctt
      601 agagaaaatc aactatctcc cagaataatg caaactttgt t
//
LOCUS       DAJTSI010000399          636 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.409, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000399 DAJTSI010000000
VERSION     DAJTSI010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.409"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            88..153
                     /locus_tag="OO327_004362"
                     /pseudo
     CDS             88..153
                     /locus_tag="OO327_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            381..629
                     /locus_tag="OO327_004363"
     CDS             381..629
                     /locus_tag="OO327_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR6020635.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          202 a          131 c          147 g          156 t
ORIGIN      
        1 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
       61 tgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
      121 ctttgtgata ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
      181 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
      241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
      301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
      361 tacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
      421 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
      481 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
      541 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
      601 agtctgctga cgagtggttt gttcactaat aaacac
//
LOCUS       DAJTSI010000400          635 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.410, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000400 DAJTSI010000000
VERSION     DAJTSI010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.410"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            18..491
                     /locus_tag="OO327_004364"
     CDS             18..491
                     /locus_tag="OO327_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6020636.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          210 a          113 c          127 g          185 t
ORIGIN      
        1 gagatgaacc ctatgcaatg tcctggttaa tatctcagtg tgctcatcag tgcactgata
       61 ataaaaagac ggagacagat gccatttacg ataaagttcg gagttcttat ttgctttcat
      121 gtattctgaa aaaaaacaaa aatgttgggc ttattcttca tgcaccatca tttgtttctg
      181 tgtctgaaaa aatcgccaga attgtgatgg caaattactc ccgtaactgg agtaattccg
      241 agttggccag tgcggtttta atgagtgaat cttctttaaa aagaagaatg tacaaagagg
      301 tcggttctat aagtacattt gtacataaaa taaagcttac agaagctatc aggaaattac
      361 gaaggacaaa tactcccatt tctgtcatat catctgagtt aggttacagt tcaccatcgt
      421 atttttctaa agtcttcttt aaatatttaa agacataccc tcaaaacata aggaagaaaa
      481 atggtcggta atgtcaccaa ttaggaggac ttgcagagcc gcttttttac gatactgaga
      541 tcaaacagac ataatctatg cggagattta cagcagggca tggttcacaa atctgacagc
      601 gatgaattat cggcactgcg ggcagaaaat gctcg
//
LOCUS       DAJTSI010000401          632 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.411, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000401 DAJTSI010000000
VERSION     DAJTSI010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.411"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(76..206)
                     /locus_tag="OO327_004365"
                     /pseudo
     CDS             complement(76..206)
                     /locus_tag="OO327_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612303.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(204..299)
                     /locus_tag="OO327_004366"
                     /pseudo
     CDS             complement(204..299)
                     /locus_tag="OO327_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365624.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          180 a          120 c          156 g          176 t
ORIGIN      
        1 ttattttttt acattatagt cggctaaatg aagtttactg taaaaaaacg atactttttc
       61 gggtggcgct tccaatcagt agaatatgtg tttttcaatg aaggagccga tgacttttcg
      121 tggatttcca cggaacgcga gaagcagatt gttttacgtg ccagccgctt gatgcgggtg
      181 cggagggtca ggttgttacg ttcaagatat agcatgtgct ggatgaagat cccttttctg
      241 gtcacctgtt tatctttcgt ggccgacggg gtgacactgt taaaatgtaa tgaacgcatc
      301 ccacacaatt aaacacggag tagtatgatg gttaaaaggg tgaaactggc aggtgtggag
      361 cctcgcagct ttccggcatc atactgccca atacgggatg cataaagcgt cctcagactg
      421 gagactccac aaatgaaata tacaccggtt ggcgttgata tggcaaaaca tgtcattcag
      481 attcacttca tcaatgagca catgtcagcg ccagtgatat aagacggtaa tggggatgcg
      541 gacgatttcc tggactctca gaagatgaaa catgtttact gaagaacaaa aaattcgcgc
      601 tattgagctt tatttcaaat atggtaagaa gc
//
LOCUS       DAJTSI010000402          631 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.412, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000402 DAJTSI010000000
VERSION     DAJTSI010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.412"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>631)
                     /locus_tag="OO327_004367"
     CDS             complement(<1..>631)
                     /locus_tag="OO327_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inositol phosphorylceramide synthase"
                     /protein_id="HCR6020637.1"
                     /translation="AGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDIPSQVFGWETAIP
                     FLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCGFFLYPLKFSFIR
                     PEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVRWRKVCGGWFLLI
                     AISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKLDQRRIKIALP"
BASE COUNT          210 a          153 c          150 g          118 t
ORIGIN      
        1 tggcagtgct attttgattc gacgttgatc aagtttctga taattccaac gacggtcgac
       61 gggcaccatc cagtcaatca acatacctac cgccagccct gtgatgacat caataaaatg
      121 atgctgccat gtcgtcagcg tcgaaatggc gatgagtaaa aaccatccgc cacagacttt
      181 acgccacctc acagccagat gctgacgaaa gtgacgccaa agtagccagc agagaataat
      241 atgcagcgaa ggagactggt tataaggcag atcaaacagt tcaagttgcg aaaatagcca
      301 tcctgtcacc ccactcactt caggacggat aaaactaaac ttcagcgggt agagaaaaaa
      361 accgcagcag gccattaccg ttgccagaat aagccggtgg acaagtcggc gttgttcgaa
      421 tgtcgagcta caaacgaaca gcgaaaatcc atataaaaga tccagactcc agtaaggaac
      481 aatagtccag ggaagaaaag ggatcgccgt ttcccaaccg aatacctgac tggggatatc
      541 atggctgtta aggtcctgaa ccgcggtgaa ctgattaaga gatccatagg tgaagaagaa
      601 aaacggggcc agcaacaata accagccagc g
//
LOCUS       DAJTSI010000403          630 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.413, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000403 DAJTSI010000000
VERSION     DAJTSI010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.413"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>630
                     /locus_tag="OO327_004368"
     CDS             <1..>630
                     /locus_tag="OO327_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
                     /protein_id="HCR6020638.1"
                     /translation="QLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVT
                     VRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQ
                     RNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLT
                     LWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQY"
BASE COUNT          147 a          160 c          178 g          145 t
ORIGIN      
        1 tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt
       61 acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct
      121 ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt
      181 ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt
      241 tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga
      301 atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct
      361 ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga
      421 ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac
      481 gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc
      541 ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg
      601 ggcaatggag cgcctgccgg cccagtatca
//
LOCUS       DAJTSI010000404          626 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.414, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000404 DAJTSI010000000
VERSION     DAJTSI010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.414"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..614
                     /gene="rspA"
                     /locus_tag="OO327_004369"
     CDS             <1..614
                     /gene="rspA"
                     /locus_tag="OO327_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCR6020639.1"
                     /translation="KFGFNEHLLHDMHHRLTPIEAARFGKSIEDYRMFWMEDPTPAEN
                     QECFRLIRQHTVTPIAVGEVFNSIWDCKQLIEEQLIDYIRTTLTHAGGITGMRRIADF
                     ASLYQVRTGSHGPSDLSPVCMAAALHFDLWVPNFGVQEYMGYSEQMLEVFPHNWTFDN
                     GYMHPGDKPGLGIEFDEKLAAKYPYEPAYLPVARLEDGTLWNR"
BASE COUNT          160 a          159 c          154 g          153 t
ORIGIN      
        1 acaagtttgg ttttaatgaa catttgctgc atgacatgca ccatcgctta acgcctattg
       61 aagcggcgcg ctttggtaaa agcattgaag attatcgcat gttctggatg gaagacccga
      121 cgccagcgga aaaccaagaa tgcttccgtc tcattcgcca acataccgtc acacccatcg
      181 cagtgggtga agtcttcaac agcatctggg actgcaaaca actgattgaa gagcaactca
      241 tcgattatat ccgcaccacg ctgacccatg caggcggaat taccggtatg cgccggattg
      301 ccgattttgc ttcgctgtat caggtacgta ctggctcaca cggtccttcc gatttgtcac
      361 cagtctgcat ggctgcggcg ctgcactttg atctgtgggt ccccaatttc ggtgtccagg
      421 aatacatggg ttattccgaa caaatgctcg aagtcttccc gcacaactgg actttcgata
      481 acggctatat gcatccggga gacaaaccgg gtcttggtat cgaattcgat gaaaagctgg
      541 cggcgaaata tccctatgaa cctgcttatc taccagtcgc acgtctggaa gatggcacgc
      601 tgtggaacag gtaaggagta agataa
//
LOCUS       DAJTSI010000405          624 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.415, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000405 DAJTSI010000000
VERSION     DAJTSI010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.415"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>624)
                     /locus_tag="OO327_004370"
     CDS             complement(<1..>624)
                     /locus_tag="OO327_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6020640.1"
                     /translation="VWRLMKRVGIKAQVGYRSPRARKGEASIVSPNRLQRQFNPDAPD
                     ERWVTDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLNALLMAVWRRNPE
                     KQVLVHSDQGSQYTSHEWQSFLKSHGLEGSMSRRGNCHDNAVAESFFQLLKRERIKKK
                     IYGTREEARSDIFDYIEMFYNSKRRHGSSEQMSPTEYENQYYQRLGSV"
BASE COUNT          140 a          173 c          140 g          171 t
ORIGIN      
        1 gacacttccg agccgttgat aatactggtt ttcatattct gtcggtgaca tctgttcgct
       61 agaaccatgc cgacgcttac tgttataaaa catttcgatg taatcaaaaa tatcactgcg
      121 ggcttcttcc cgcgttccgt agatcttttt ctttatccgt tcacgtttca acaactggaa
      181 aaaactttct gcaaccgcat tatcatggca gttaccgcga cggctcatgc taccctccag
      241 gccgtgtgat ttcaggaacg actgccactc atggcttgtg tactgactgc cctgatccga
      301 atgaaccagc acctgttttt cgggattacg ccgccataca gccatcagca gtgcgttcag
      361 gacaatgtcc tttgtcatcc gggattgcat ggaccagccg ataattttgc gtgagaacag
      421 atcaacaaca acggcaagat acagccagcc ttcgtgggtc ctgatgtagg ttatgtccgt
      481 tacccaacgc tcatcaggag catccggatt gaactgtcgc tggagcctgt tgggtgacac
      541 gatactggcc tcgcctttac gtgcccgcgg gcttcggtat ccgacctgag cctttattcc
      601 gacacgtttc atcagtctcc agac
//
LOCUS       DAJTSI010000406          622 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.416, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000406 DAJTSI010000000
VERSION     DAJTSI010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.416"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..251)
                     /locus_tag="OO327_004371"
     CDS             complement(<1..251)
                     /locus_tag="OO327_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR6020641.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKILLSNENVDVQNVWRDQQETLLTYLQ"
     gene            complement(272..>622)
                     /locus_tag="OO327_004372"
     CDS             complement(272..>622)
                     /locus_tag="OO327_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6020642.1"
                     /translation="TVHKCTFVFSPQVIKQRQFSGPHYKVDSIRESWTSILKRAGLRH
                     RKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLNRR
                     LSESVPCMPHKKAG"
BASE COUNT          140 a          144 c          141 g          197 t
ORIGIN      
        1 ctttgcaggt aagtcagtaa tgtttcttgc tgatcgcgcc agacgttctg cacatcaaca
       61 ttttcattac tgagcagtat tttaactttg ctgacctgca cgccgttgtc gatccagcgt
      121 ttgatctcgc ggatccggtc aatatcggca tcgttgaaca gccgatgacc gccgtctgtc
      181 cgttgcggtt tcagcaatcc gtaacgcctc tgccacgcgc gtaacgtgac agggattaaa
      241 ggtaaaccaa ttgattttgc aagtttttat tttaccccgc tttcttatgg ggcatgcatg
      301 ggacactttc agatagtctt ctgttaagaa gttctatctg ttcgtgattg ttgtctttca
      361 tccatgctcc gtaaacattg aataccattt gtgcgtttgt gtggcccatc tggcttgcga
      421 taaaactagg attagctcct gcggcaagtg accagcatgc ataggtatgc ctggattgat
      481 atgattttct gtgtctcaga cctgcgcgtt ttaagatact tgtccatgac tccctgatgg
      541 agtcaacctt atagtgcggt ccggaaaact gccgctgttt tattacctga ggactaaaaa
      601 caaaagtgca tttatgcaca gt
//
LOCUS       DAJTSI010000407          619 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.417, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000407 DAJTSI010000000
VERSION     DAJTSI010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.417"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>619
                     /locus_tag="OO327_004373"
     CDS             <1..>619
                     /locus_tag="OO327_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HCR6020643.1"
                     /translation="TKPLFLLRKAKKSSGEPDVVLWASDDFESTSTTLDYLLVKSGKK
                     LSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHHQDNA
                     QETKELAGGQEENAQADAHEDCQDCEVSVATLRFTQRLLHIFTYAAGDLKYLHHATRE
                     QRKHITALEMDQENSYVQNLLLAIRGMAEPTTLDNAALLRLTDAIK"
BASE COUNT          178 a          165 c          144 g          132 t
ORIGIN      
        1 cacaaaacca ctcttcctgt tacggaaagc gaaaaaatca tccggtgaac ctgacgtcgt
       61 cctgtgggca agcgacgatt ttgaatcgac cagcaccact ctggactatc tgctcgttaa
      121 gtcaggtaaa aaactgagca actattttaa agctgttgcc acaaattttc ctgtcgttaa
      181 tgaccttccc cctgaaggtg agatcgattt tacctggagt gaacgctatc aactcagcaa
      241 agactccatg acatgggaac taaaaccggg agcagcgcca gacgacgttc accaccagga
      301 taatgctcaa gaaaccaaag aactggcggg aggccaggaa gaaaacgcgc aggcagacgc
      361 ccacgaggat tgccaggatt gcgaagtctc tgtagccact ttgcggttca cacagcgtct
      421 tctgcacatt tttacgtatg ctgccgggga tctgaaatac ctgcatcatg ccacccgtga
      481 acaacgcaaa cacattactg ctcttgagat ggatcaggaa aacagctatg tccagaatct
      541 gctgttggcc atacgcggca tggcagaacc gacaactctg gataatgccg ccctgctccg
      601 cctgactgat gcaattaag
//
LOCUS       DAJTSI010000408          614 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.418, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000408 DAJTSI010000000
VERSION     DAJTSI010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.418"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..614
                     /locus_tag="OO327_004374"
                     /pseudo
     CDS             1..614
                     /locus_tag="OO327_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
BASE COUNT          146 a          153 c          184 g          131 t
ORIGIN      
        1 accaggaagg aagtcttact gctgtggccg ccggagaaca ggttgttcct gcctctgaac
       61 ttgctgccgc catgaagcag attaaagaac tccagcgcct gctcggcaag aaaacgatgg
      121 aaaatgaact cctcaaagaa gccgttgaat atggacgggc aaaaaagtgg atagcgcacg
      181 cgcccttatt gcccggggat ggggagtaag cttagtcagc cgttgtctcc gggtgtcgcg
      241 tgcgcagttg cacgtcattc tcagacgaac cgatgactgg atggatggcc gccgcagtcg
      301 tcacactgat gatacggatg tgcttctccg tatacaccat gttatcggag agctgcccac
      361 gtatggttat cgtcgggtat gggcgctgct tcgcagacag gcagaacttg atggtatgcc
      421 tgcgatcaat gccaaacgtg tttaccggct catgcgccag aatgcgctgt tgcttgagcg
      481 aaaacctgct gtaccgccat cgaaacgggc acatacaggc agagtggccg tgaaagaaag
      541 caatcagcga tggtgctctg acgggttcga gttctgctgt gataacggag agagactgcg
      601 tgtcacgttc gcgc
//
LOCUS       DAJTSI010000409          601 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.419, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000409 DAJTSI010000000
VERSION     DAJTSI010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.419"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..108
                     /locus_tag="OO327_004375"
     CDS             <1..108
                     /locus_tag="OO327_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'') family aminoglycoside
                     nucleotidyltransferase"
                     /protein_id="HCR6020644.1"
                     /translation="QAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            244..601
                     /locus_tag="OO327_004376"
                     /pseudo
     CDS             244..601
                     /locus_tag="OO327_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          144 a          157 c          159 g          141 t
ORIGIN      
        1 caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa
       61 tttgttcact acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg tctaacaatt
      121 cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg cactaagcac
      181 ataattgcgg tgatgctgcc aacttactga tttagtgtat gatggtgttt ttgaggtgct
      241 ccagtggctt ctgtttctat cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt
      301 aacggcaaaa gcaccgccgg acatcagcgc tatctctgct ctcactgccg taaaacatgg
      361 caactgcagt tcacttacac cgcttctcaa cccggtacgc accagaaaat cattgatatg
      421 gccatgaatg gcgttggatg ccgggcaacc gcccgcatta tgggcgttgg cctcaacacg
      481 attttccgcc atttaaaaaa ctcaggccgc agtcggtaac ctcgcgcata cagccgggca
      541 gtgacgtcat cgtctgcgcg gaaatggacg aacagtgggg atacgtcggg gctaaatcgc
      601 g
//
LOCUS       DAJTSI010000410          587 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.420, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000410 DAJTSI010000000
VERSION     DAJTSI010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.420"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            57..554
                     /locus_tag="OO327_004377"
                     /pseudo
     CDS             57..554
                     /locus_tag="OO327_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          142 a          144 c          181 g          120 t
ORIGIN      
        1 gccagccccg tcccgcccga tagagcgcgg catagcgggc cccggattac ttgcgtgcga
       61 cggcagaaga agccctgaag cgaatcagtg aactgtacgc catcgaggat gaaatacggg
      121 gattaccaga gtcagagtgt cttgcagtca ggcagcagcg aagcaaagcg ttactgacgt
      181 cgctgcatga atggatggtg gagaagaatg gcacgctgtc gaaaaaatcc agactgggcg
      241 aagcattcag ctatgtactg aatcagtggg acgccctctg ttattacagt gatgacggtc
      301 tggcggaggc ggacaataac acagcggaaa gagcgcttcg tgcagtctgt ctcggaaaga
      361 aaaattacgt gttcttcggt agcgatcacg gcggcgagcg tggtgcactg ctgtacgggc
      421 tgatcggcac ctgccgtctg aacggtatcg atccggaagc gtatctgcgc catattctga
      481 gcgtactgcc ggaatggcct tccaaccgag ttgacgacct cctgccatgg aaggtggtgc
      541 taccatcagg ataatcgtca atacggtgtt cggtgaacgt ttacttg
//
LOCUS       DAJTSI010000411          581 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.421, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000411 DAJTSI010000000
VERSION     DAJTSI010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.421"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(141..>581)
                     /locus_tag="OO327_004378"
     CDS             complement(141..>581)
                     /locus_tag="OO327_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR6020645.1"
                     /translation="GGKTGNMFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLR
                     DMINGKQKSWLAHCTDAEALCIDRVISEVLAEHPGLISVLRQRYEGRGMTKCKMAELL
                     NDAHPKWSLRTCERRIEHWLKVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          147 a          163 c           99 g          172 t
ORIGIN      
        1 caccagcacg tttaacgtcc tgtgccgttt ttcaggtata aaaaaaaccg cataaagcgg
       61 gctctttcaa atgtccatgt ctgctattcg cctcgcggta cagctttgcg aagcgtacaa
      121 gaattgaagc agtttttacg tcaaaaagca ataacttttt tctctatacc aaaagccata
      181 accattggtt tgtacaaaat aaattctgcc acctttagcc aatgctcaat gcgtctttca
      241 caggttctta aactccattt cgggtgtgca tcattcagca gttcagccat tttgcatttg
      301 gtcatccccc gcccctcata ccgttgccga aggacgctaa tcaatcctgg atgctctgcc
      361 agcacctcac ttatgacccg atcaatacat aacgcctctg catcagtaca atgcgccagc
      421 cagctctttt gcttaccgtt aatcatatcc cgcaaaaaag cctcaagttc agacttgttc
      481 agacctgctt ttttcatcct ccggagcgcc tcgttaattg ccgtttttgt cagcttttta
      541 gaggtcaaca tcaggttaaa catattcccc gtcttaccgc c
//
LOCUS       DAJTSI010000412          554 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.422, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000412 DAJTSI010000000
VERSION     DAJTSI010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.422"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            98..>554
                     /locus_tag="OO327_004379"
     CDS             98..>554
                     /locus_tag="OO327_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005062103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020646.1"
                     /translation="MAGVLEGRLSGGTAAEFCNSAVVALQAAGLDVCREYPVPERGDG
                     CGGRIDIVVTDRNGVRCGIELDRNSPRQKSLLKIGAVETGICVLRRSDIARHTEQGIL
                     VIGGAVRQKKFDPLSVDLPDWLSETLWHEWVQFRQALRKPIRTELGANGA"
BASE COUNT          131 a          119 c          174 g          130 t
ORIGIN      
        1 caacacaaag gcaactaata aagatacaga taaagatcta aaaacagatc taacccatcc
       61 caaacccttc ccttccggaa gggagtttcg ggattttgtg gctggagtgc ttgaggggag
      121 attatctggc ggtactgcag cggaattttg taattctgcg gtggttgcgt tgcaggctgc
      181 tggcctggat gtctgtcgtg agtatccggt gccagagcgt ggtgacggtt gcggagggcg
      241 gattgatatc gtcgtgactg acaggaacgg tgtccggtgt gggatcgagc ttgaccgaaa
      301 ttctccgcga cagaaatcac tgctcaaaat cggtgctgtt gaaaccggga tatgtgtctt
      361 gcggcgcagt gatatcgcaa ggcacaccga gcagggaatt ctggttatcg gtggggctgt
      421 tcgccagaaa aaatttgacc cgttgtcagt tgatctgccc gactggttgt cagaaacact
      481 ctggcatgag tgggtccagt tcaggcaggc attgcgaaaa ccgattcgaa cggagctggg
      541 cgctaacggg gcga
//
LOCUS       DAJTSI010000413          551 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.423, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000413 DAJTSI010000000
VERSION     DAJTSI010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.423"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>551)
                     /locus_tag="OO327_004380"
     CDS             complement(<1..>551)
                     /locus_tag="OO327_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MolR family transcriptional regulator"
                     /protein_id="HCR6020647.1"
                     /translation="VEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAETRAADKN
                     TDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVSQRDNKT
                     ATFDFSTCSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVREDVMDQ
                     IVQEGSLEYATDVIIHLQQIDIK"
BASE COUNT          134 a          117 c          135 g          165 t
ORIGIN      
        1 atttaatatc aatctgttga aggtgtatta ttacatcagt agcatattcc agactgcctt
       61 cctggacgat ctgatccata acatcctcac gcactttata tcttgtgcat ttcatttcaa
      121 gtgccgtgag tacagccatt actggtgatg gtagctgtgt tgttttcagg ccgtcgatct
      181 gactgatcgc ttgggcgacg gtgttttgcc attctaaaga acaggtgctg aagtcaaatg
      241 tggctgtttt attatcccgt tgcgacacgc tacaggctaa gcgacgtaat tgggtcaatt
      301 tgtctgcttg ctgaacagga actccgggaa agcggcgatg acttaacgtg gtgtgtacaa
      361 cttcgggcgg aaaaacgaca gcaatatctt tcgccagcca aggtaaaaca gcatctgtat
      421 ttttgtcagc cgcacgggtt tctgctacga aagagggttg ttcctgaata atggtggata
      481 agtccgctac cttgagagag tccgttaccg tttgggaagg ttgcaaagaa ttatctttcg
      541 cttgctccac a
//
LOCUS       DAJTSI010000414          551 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.424, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000414 DAJTSI010000000
VERSION     DAJTSI010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.424"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>551
                     /locus_tag="OO327_004381"
     CDS             <1..>551
                     /locus_tag="OO327_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCR6020648.1"
                     /translation="APTLRIRDPHKDEKMAAIHKALDECSAEHPVFYEDEVDIHLHPK
                     IGADWQLRGQQKRVVTPGQNEKYYLAGALHSGTGKVSYVGGNSKSSALFISLLKRLKA
                     TYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRGIYQPVYSPWVNHVERLWQALHDT
                     ITRNHQCRSMWQLLKKVRHFMET"
BASE COUNT          163 a          134 c          147 g          107 t
ORIGIN      
        1 tgcgccaact ctgcgtatcc gtgacccgca taaagatgaa aagatggcag caatccataa
       61 agcactggac gaatgcagcg cagagcatcc ggtcttttat gaagatgaag tggatatcca
      121 tcttcatccc aaaatcggtg cggactggca actgcgcgga cagcaaaaac gggtggtcac
      181 gccgggacag aatgaaaaat attatctggc cggagcgctg cacagcggga caggtaaagt
      241 cagctatgtg ggcggcaaca gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct
      301 taaagcgaca taccgtcgtg cgaaaaccat cacgctgatc gtggacaact acattatcca
      361 caaaagccgg gaaacacaga gctggctgaa ggagaacccg aagttcaggg gcatttatca
      421 gccggtttac tcgccatggg tgaatcatgt tgaacggcta tggcaggcac ttcacgacac
      481 aataacgcgt aatcatcagt gccgctcaat gtggcaactg ttgaaaaaag ttcgccattt
      541 tatggaaacc g
//
LOCUS       DAJTSI010000415          549 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.425, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000415 DAJTSI010000000
VERSION     DAJTSI010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.425"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..142)
                     /gene="csgC"
                     /locus_tag="OO327_004382"
     CDS             complement(<1..142)
                     /gene="csgC"
                     /locus_tag="OO327_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR6020649.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLR"
     gene            complement(201..>549)
                     /gene="csgA"
                     /locus_tag="OO327_004383"
     CDS             complement(201..>549)
                     /gene="csgA"
                     /locus_tag="OO327_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HCR6020650.1"
                     /translation="NNSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHGGGNGAD
                     VGQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVT
                     QVGFGNNATAHQY"
BASE COUNT          146 a          127 c          132 g          144 t
ORIGIN      
        1 cgcgcaggga caatatttgt actctgcaca gacaagattg agtaagagta acttcaggaa
       61 taatggtgta catatcccct tgctgggtcg tattaaaggt tatctggctg gaaagtgccg
      121 caaggagtaa taacgcattc atattcttct cccgaaaaaa aacagggctt gcgccctgtt
      181 tctgtaatac aaatgatgta ttaatactga tgagcggtcg cgttgttacc aaagccaacc
      241 tgagtcacgt tgacggagga gttcgatgca gtctggtcaa ctgcagcacc gttgccacca
      301 ccgaactgtt taacagtcat ttcagaattt ttgccgttcc actgatcaag agtggcgctg
      361 ttaccgaagc cacgttgggt cagatcgatt gagctgtcat ctgagccctg accaacatct
      421 gcaccattac caccgccatg ctgggtaata gtcaagtcag agttacgggc atcagtttgc
      481 agagcaagtg cagagttacc gccaccgtac tggtaaatgt tcagctctga atttgggccg
      541 ctattatta
//
LOCUS       DAJTSI010000416          533 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.426, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000416 DAJTSI010000000
VERSION     DAJTSI010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.426"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
BASE COUNT          173 a           78 c          107 g          175 t
ORIGIN      
        1 ttatatgaat atttcagagt tggagttcga atgttgacac ctgagtattt cctgctaaaa
       61 atagccctgc tttacggatg gttgtattgc aataacacga aagtagtaag agattaaagg
      121 tatctgtcag cgataactct gcaagtgatg acattttgta aaccaaataa tgtaaatcag
      181 caatgtatct tttcacttaa ttataacaaa tgtggtattc actaagaatc tgatacgagc
      241 ttacatcacg actattttag tctcatcgcg ggatacaggg atgcaccgat ggatctcagc
      301 acaccgtaat gcaatacctg aacaaataga acagtaaagt ggttcagtat taatgaaagc
      361 tcttattttc cgttgggaaa acttaaaata gatgagaagg ctttttgatt tcttatgtga
      421 tctcatttcg agtattgaga aatttgtgat ttttaaattt ttcaataagc cctgtaaggg
      481 attggggttt tatgagaaat aaagagacgt attgtctttg tttaaacggt acg
//
LOCUS       DAJTSI010000417          519 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.427, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000417 DAJTSI010000000
VERSION     DAJTSI010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.427"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            368..>519
                     /locus_tag="OO327_004384"
     CDS             368..>519
                     /locus_tag="OO327_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6020651.1"
                     /translation="MSQQPQQPQQEISIINLDQLVSMTSVEIAELTGKEHRNVLRDIR
                     KMLEEL"
BASE COUNT          129 a          137 c          113 g          140 t
ORIGIN      
        1 tagggtctct ctataggtct tctctatggt ctctctatag gtctctctag ggtctcctct
       61 aggcattacc cccagaagct aactctatgg ccctctatgg tcctctatgg ccctgtacgt
      121 cattctttag gatgccctgt agtttcgtat agcctgatgc ctagagagac cctagagaga
      181 ccctagagtg gctcctagag agaccctccc agtagctccg cacagctaca tcgtcatcat
      241 catagtattc aatctcaaag tcattctgtt tgcatcctgt tgtcctgctg gtctcccttc
      301 gcttggggac tttgagtagg tcagtggggg tgcgacttca gggtgcaaac aaaaaggaaa
      361 tcaagcgatg tctcaacaac cacaacaacc acaacaagaa atcagcatca ttaacctcga
      421 ccagcttgtg tccatgacct cagttgaaat tgctgagctg acaggcaagg agcaccgaaa
      481 tgtccttcgt gatattcgta agatgctcga agaacttaa
//
LOCUS       DAJTSI010000418          511 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.428, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000418 DAJTSI010000000
VERSION     DAJTSI010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.428"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..511
                     /locus_tag="OO327_004385"
                     /pseudo
     CDS             1..511
                     /locus_tag="OO327_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          127 a          128 c          141 g          115 t
ORIGIN      
        1 acaccacggg ccggacgccc atgggctgag caccgtaaaa tcatcaatgg catgttctgg
       61 gtgttatgtt ccagtgcacc atggcgcgat ttacccgaac gatatggggc atggaaaact
      121 gtttataacc gctttaaccg atggtcaaag tcaggcgtga ttaatattat tttcaacagg
      181 ttgctttcgc tacttgatgc aaacggcttt attgactggt ctgccaccgc gctggatggc
      241 agtaatatcc gggcgctgaa atgtgccgcc ggtgctcaaa aaaacatccc gatatcgacg
      301 gagataatgg gccgggtcgc tctcgcggcg gttttggcac caaaatccat ctggcaacag
      361 acggaagtgg cctcccgtta aatatcgtgc taagccccgg acaagctcat gaaagccagt
      421 tcgcgcaacg acttctggac gggattggcg ttcagcgtca gaacggcagc atgaaacgcc
      481 gtggacatgc ggtactggct gataaagcgt a
//
LOCUS       DAJTSI010000419          488 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.429, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000419 DAJTSI010000000
VERSION     DAJTSI010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.429"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(249..>488)
                     /locus_tag="OO327_004386"
     CDS             complement(249..>488)
                     /locus_tag="OO327_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR6020652.1"
                     /translation="APIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQG
                     LSMSSDEHADEYSRYIVDGAAVAEMKGIIERHQAK"
BASE COUNT          132 a          116 c          106 g          134 t
ORIGIN      
        1 ttgctaagga ataacccagt tgctatttag aatatatatt tatcagtttt agcaatttaa
       61 atcccataat caatgtgaat ataaaaaata tttttagcac aacgctatgc gcactgtcac
      121 ccgctcaaca gatcacggtt atcgttcgtt ttttatactg ctcagggata aaaaaaggcc
      181 cctgttgaaa ttgcaggggc ctggtacgag caagcatcat attgggcgac atgatgcaac
      241 ggtaaaaatc atttggcctg atggcgttca atgattcctt tcatttcagc aaccgccgcc
      301 ccgtctacga tataacgcga atactcgtcc gcgtgttcat ctgatgacat ggacaatcct
      361 tgattacgat aacgcatcgg tgaggcttgc cacgctcccg cggagctatg gacttccagc
      421 actccggcat cgaggaagtg gtgcaggttt tctgcacgga ctcctgctcc agccataatg
      481 attggagc
//
LOCUS       DAJTSI010000420          485 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.430, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000420 DAJTSI010000000
VERSION     DAJTSI010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.430"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..485
                     /locus_tag="OO327_004387"
                     /pseudo
     CDS             1..485
                     /locus_tag="OO327_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          140 a          126 c          124 g           95 t
ORIGIN      
        1 tcagggcaaa acaccaaaag cctcccccat taccccggaa caaattgaaa tccgtgagct
       61 caggaaaaag ctacaacgca ttgaaatgga gaatgaaata ttaaaaaagg ctaccgcgct
      121 cttgatgtca gactccctga acagttctcg ataatcggga aactcagagc gcattatcct
      181 gtggtcacac tctgccatgt gttcggggtt catcgcagca gctacagata ctggaaaaac
      241 cgtcctgaaa aaccagacgg cagacgggct gtattacgca gtcaggtact tgagctacat
      301 ggcatcagcc acggttcggc cggagcaaga agcatcgcca caatggcaac ccggagaggc
      361 taccagatgg gacgctggct tgctggcagg ctcatgaaag agctggggct ggtcagctgt
      421 cagcagccga ctcaccggta taaacgtggt ggtcatgaac atgttgctat ccctaactac
      481 cttga
//
LOCUS       DAJTSI010000421          483 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.431, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000421 DAJTSI010000000
VERSION     DAJTSI010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.431"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..483
                     /locus_tag="OO327_004388"
                     /pseudo
     CDS             1..483
                     /locus_tag="OO327_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          177 a           81 c           95 g          130 t
ORIGIN      
        1 tcgagtaata atgaatatga cctgtttaaa aatagagaaa cctcactgtg gaaacgcaaa
       61 gtaccagaaa gcgcagcgct ggcaatgacg ggaacagtat ataaattatt tggtaatcgc
      121 ttactcaacg cccgcaatca gttagataaa aatctcctcg aaatggctat gcaaatggtt
      181 tatggcaaag atatggtgac aaagtccggg gatatattga ttgatataca attaaataag
      241 gatggcagtc tccagagtaa gcactacaca cttgatgtag gtgaagttat aaacagttat
      301 aatattgacc ctctttgtga atgcttcaag tattaatcaa acttacttac atgataaaaa
      361 tcctggtgac gttattagcc tgtatggcac tttagataac attccccttg tagttaagga
      421 atgcacgggt aaaaatcatc aaattaatgt taaagccaaa ttgccacaag aacgaagtga
      481 aaa
//
LOCUS       DAJTSI010000422          480 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.432, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000422 DAJTSI010000000
VERSION     DAJTSI010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.432"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>480
                     /locus_tag="OO327_004389"
     CDS             <1..>480
                     /locus_tag="OO327_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6020653.1"
                     /translation="CSFANYRAQNDGQRHALSQAKSIADELMTGCTNFVFSGKPGTGK
                     NHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQELCGVDLLVLDEI
                     GMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERVMDRMVMNGGRWV
                     "
BASE COUNT          117 a          103 c          154 g          106 t
ORIGIN      
        1 gtgttcgttt gcgaattacc gggcgcagaa cgacggtcag cgacatgcac tgagtcaggc
       61 gaaatccata gctgacgaac tgatgacagg ctgtacgaat tttgtgttca gcggtaagcc
      121 cggcaccgga aaaaatcatc ttgcagccgc gattggcaac cggctgatgg cgaaggggcg
      181 tagcgtgatt atcgtcaccg tgtccgatgt catgagcgtg ttgcatgaga gctacgacaa
      241 cggcaaatcc ggtgaaaaat ttttacagga gctttgtggt gttgacctgc tggtcctgga
      301 tgaaattggc atgcagcggg atacgaaaaa cgagcaggtg gtactgaacc agattgttga
      361 tcgccggacg gcatcgttac gcggtgtggg gatgctgaca aatattaacc atgcagcgat
      421 gaatacactt ctcggcgagc gggtgatgga tcgcatggtc atgaacggcg ggcgctgggt
//
LOCUS       DAJTSI010000423          472 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.433, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000423 DAJTSI010000000
VERSION     DAJTSI010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.433"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>472
                     /locus_tag="OO327_004390"
     CDS             <1..>472
                     /locus_tag="OO327_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCR6020654.1"
                     /translation="LDAGGLRDIEVEAVTKMLACGTRILGVKEYICDKPECPHVRYVT
                     NSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTQWPVFESNRWLLNDVC
                     RLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKL"
BASE COUNT          101 a          130 c          144 g           97 t
ORIGIN      
        1 tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct
       61 gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag tgcccccacg
      121 tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga aaaaaggcca
      181 cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtacacctgg
      241 tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg ctgctgaatg
      301 acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg gggcaggaac
      361 ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac
      421 atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag ct
//
LOCUS       DAJTSI010000424          468 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.434, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000424 DAJTSI010000000
VERSION     DAJTSI010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.434"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(7..402)
                     /locus_tag="OO327_004391"
     CDS             complement(7..402)
                     /locus_tag="OO327_004391"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020655.1"
                     /translation="MNVVSNTQLLEQRIADFFTLSDEHKKARVLLDTLACSCPARIFG
                     GMVRDLGLYGVDGFSSDLDIVIGRSREELFQTLAELPVKQLRFNKFGGIRFRYHDFEF
                     DIWNLNETWAFQEKLIFCEDESSLLNEVA"
BASE COUNT          141 a          112 c           98 g          117 t
ORIGIN      
        1 ttaaactcag gctacctcat ttaacagact tgattcatcc tcacaaaata taagtttttc
       61 ctggaatgcc catgtctcat tgaggttcca gatatcaaat tcgaagtcgt gataacgaaa
      121 acggatccca ccaaacttat taaaacgcag ttgtttgacg ggcagttcag ccaatgtctg
      181 gaagagttcc tcacgggagc gaccgatgac gatatcaagg tctgaagaga atccatcgac
      241 gccatacaag cctaagtcac gcaccatccc gccaaaaatc ctggccggac aggaacaggc
      301 cagagtatca agtaatactc tggctttctt atgctcatca ctaagcgtaa aaaaatcggc
      361 gatacgctgt tcaagcagtt gggtgtttga gaccacattc atacagtgat tttagctcat
      421 cgtgaaggcg cattaaataa ctatccgtca tgcctgagat tgcgtcgg
//
LOCUS       DAJTSI010000425          467 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.435, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000425 DAJTSI010000000
VERSION     DAJTSI010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.435"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>467
                     /locus_tag="OO327_004392"
     CDS             <1..>467
                     /locus_tag="OO327_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020656.1"
                     /translation="SGSTLISRDNKLVENFIDTLENWRDQKQIISVICKDGIKIDDSI
                     ITSFKASKDVGISNGLRIQLTFQEINFKAIVGQTDVSAATGRTSTTNDGGATSKKNTG
                     NTTTSLGSPMLTCKELFSYSASELSDEALKARVTCSKSVSVKNGESTFTASAN"
BASE COUNT          148 a           96 c          102 g          121 t
ORIGIN      
        1 aatccggtag tacccttatc tcacgagata ataagctggt ggagaacttc atcgacactc
       61 ttgaaaactg gcgtgaccag aagcagatta tttcagttat ctgtaaagat ggtattaaga
      121 ttgacgactc cattattaca agctttaaag cctctaaaga tgttggaatt tcaaacggac
      181 tgagaatcca gctaactttt caggaaatta acttcaaagc tattgtaggg caaactgatg
      241 tttcagcagc cactggacgg acttctacca cgaatgatgg cggtgctacc agtaaaaaga
      301 acactggcaa cactacgaca agtcttggaa gccctatgct gacctgtaaa gagcttttta
      361 gctattcggc aagtgagtta tcagatgaag cgttaaaagc ccgtgtaacg tgctctaaga
      421 gcgttagcgt taaaaatggt gagagcacct tcacagcctc agctaat
//
LOCUS       DAJTSI010000426          461 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.436, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000426 DAJTSI010000000
VERSION     DAJTSI010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.436"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
BASE COUNT          164 a           78 c           78 g          141 t
ORIGIN      
        1 ggtcatgttt ttgactcatg gttttctcta cttgtcaagg ataattaata cgactcacta
       61 tagggagaag ggacacaaaa agttttctct tctaggacaa ctaaccgtaa cttatcgcat
      121 cctttcaggg gcgggatgac caattacaga caccaagcgt tctacttaaa gtgaatgctt
      181 cctgtgtgct ctgggtcagt cgataagtga atagcaataa gacacaatca tcttaattat
      241 ggtctctagt ccctacaggg gcttaactta aagttaactt aaagacacaa ttaaagatta
      301 acttatagta ttacttaaag ttaacttaaa gacacaatta gagattaact ttaagtatta
      361 aataaaagat ttaattaaag accaattaaa gattaactct aagggaatac cttaaggggt
      421 gatgatattt ataataatca atcaccaaac ctataggtct t
//
LOCUS       DAJTSI010000427          459 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.437, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000427 DAJTSI010000000
VERSION     DAJTSI010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.437"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>459)
                     /locus_tag="OO327_004393"
     CDS             complement(<1..>459)
                     /locus_tag="OO327_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HCR6020657.1"
                     /translation="LSNRVASYGAIMAALPYMQLYIADYLADTMHLSAEEHGAYLLLM
                     FNYWQTGKPIPKNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVRE
                     KLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVGVVFEHDAGFA"
BASE COUNT          106 a          117 c           90 g          146 t
ORIGIN      
        1 gggcaaatcc agcatcatgt tcgaacacca ctccaacatc tgtttgaaca cctgttaaat
       61 ttctctcttg ttttgtttga acatctgctt cctttctgct tcttctggcc tgaacagatg
      121 cttttcctgc ggctgatttt ttggttaatt tttctctgac agatgccaga tcttcctcaa
      181 tccgaagatg catccattcg tcgccgttat cacaaaaaaa ctcccgcaag gatggttcaa
      241 catcaaccca tcgctcgtta gtcagacggg caatttttgc caacctgttt ttgggtattg
      301 gctttcctgt ttgccagtaa ttgaacatca gcaacaaata cgcgccgtgc tcctctgctg
      361 acaaatgcat ggtgtcagcc aggtaatcag ctatgtacag ttgcatgtat ggtaatgcgg
      421 ccataattgc cccgtatgat gctacccggt tgcttagaa
//
LOCUS       DAJTSI010000428          454 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.438, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000428 DAJTSI010000000
VERSION     DAJTSI010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.438"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
BASE COUNT          144 a           83 c           76 g          151 t
ORIGIN      
        1 tctaattata ttttaaagaa atctgtcttt atggcagatt tctttaatct catataattc
       61 ttataaaaaa tataatattt aactcgtcat attgcttata aaaaatcccc gtccccagag
      121 aaataatatt tattaaaatg gcaggtcttc tttttctgat taccgaagac aaagtggcaa
      181 ttacgtatag tttcccgata aagacgcgac agctacgtcc cgcataaggc atttttctct
      241 ttatctttgt acggtacttc atggaacaga atttttgacc ttgtgaatcg tgatatctgt
      301 tggggagggg caatttgctc actgaagcgt gagactcgat taagcgcacg aaacacagaa
      361 atcaaaaaac ccggccactt ttttacaaag tggccgggta aaaataattt ttatttttta
      421 actgtcctga gtctcataga gatgtctcaa aact
//
LOCUS       DAJTSI010000429          443 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.439, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000429 DAJTSI010000000
VERSION     DAJTSI010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.439"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            213..>443
                     /locus_tag="OO327_004394"
     CDS             213..>443
                     /locus_tag="OO327_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR6020658.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDSRRTVEIHKLDLSDWQAFNKLATKCNAYDG"
BASE COUNT          141 a          105 c           61 g          136 t
ORIGIN      
        1 tatcgaacct cgcttaccaa cgcgttgtca gtcatctccc ccctctctgg tgcatcgtta
       61 tatccgtgat gagcgatttt tgatattaat atctttcacc atccgctatt ttcaccactt
      121 cagaaaacaa acttattttt attaaaatca attctttttc tttgaacatt caactgcgat
      181 atttgtaaaa acattaaaca ggcaacaata aaatgacatt accaacaact atttattcat
      241 tccccgcata tttaccccgt ttttcgtcca ccgacaaacc agcaaaatta aaatttcatc
      301 aatacgcacg cgctacatta ctgtcaaatc gtggaagaga tcataattgc gatagcagac
      361 gtactgttga gatccacaaa ctagatctct ctgactggca agcattcaat aagttagcaa
      421 cgaagtgtaa tgcttacgat gga
//
LOCUS       DAJTSI010000430          434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.440, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000430 DAJTSI010000000
VERSION     DAJTSI010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.440"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            35..434
                     /locus_tag="OO327_004395"
                     /pseudo
     CDS             35..434
                     /locus_tag="OO327_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          100 a          112 c          114 g          108 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggac
//
LOCUS       DAJTSI010000431          430 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.441, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000431 DAJTSI010000000
VERSION     DAJTSI010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.441"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>430
                     /locus_tag="OO327_004396"
     CDS             <1..>430
                     /locus_tag="OO327_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR6020659.1"
                     /translation="YASAGLLARILVSKYMEHIPLYRQSEIYARQGVELSRNTMVRWV
                     SEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVRDDRNAGS
                     SLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVL"
BASE COUNT          109 a          101 c          127 g           93 t
ORIGIN      
        1 tatgccagtg cagggttact tgcacggatc ctggtcagca aatatatgga acatatccct
       61 ttatatcgcc agtcagaaat atacgcgcga cagggcgtgg agctgagccg taataccatg
      121 gtgcgctggg tatcagaaat ggcagacaaa ctccgtcctc tgtatatagc gctgaatgac
      181 tatgttctgg aggcaggaaa ggtgcacgca gatgacactc cggtgaaagt actggccccg
      241 gggaacggaa agacgaaaac gggtcgtctg tgggtatacg tcagggatga tcgtaatgcg
      301 ggttcatccc tgccggcagc cgtctggttc gcgtattcgg cagatcgcaa aggagaacat
      361 ccgcagctcc acctggcaaa gtatcagggc gtactgcagg ctgatgccta tgcaggttat
      421 aacgtactgt
//
LOCUS       DAJTSI010000432          424 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.442, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000432 DAJTSI010000000
VERSION     DAJTSI010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.442"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..269)
                     /locus_tag="OO327_004397"
     CDS             complement(<1..269)
                     /locus_tag="OO327_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020660.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQR"
BASE COUNT           93 a           99 c          133 g           99 t
ORIGIN      
        1 atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg gagcctgacg
       61 aagattatcc agtgacgata atcgagactg agggctttct taatctcgta tccgtgtctg
      121 cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg ctgatacccg
      181 tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc agagttatcc
      241 gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag caggtgccag
      301 ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa aacccgccga
      361 agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc gagggatttc
      421 tccc
//
LOCUS       DAJTSI010000433          424 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.443, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000433 DAJTSI010000000
VERSION     DAJTSI010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.443"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>424)
                     /locus_tag="OO327_004398"
     CDS             complement(<1..>424)
                     /locus_tag="OO327_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6020661.1"
                     /translation="DKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSIS
                     ASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYY
                     EFLRLGREGYTKVQNASYQVAAYLADEIAKQGPYEFICT"
BASE COUNT          101 a          119 c          119 g           85 t
ORIGIN      
        1 gtacagatga actcatacgg cccctgtttg gcgatttcat ccgccagata agcggcaacc
       61 tggtaagagg cgttctgtac tttggtatag ccttcacgac cgaggcgcag gaattcatag
      121 tactgtgcaa ttacctgacc cgccgggcgg gagaagttga tggcaaaagt accaatttga
      181 ccacccaggt agtcaacgtt gaacaccagt tcctgcggca gcgcttcttc gtcacgccag
      241 ataacccagc cgcagcccag cggagccaga ccgaatttat ggcctgaagc actgatcgat
      301 ttcacacgcg gcaggcggaa gtcccagacg atatccgggg cgacgaacgg tgccaggaag
      361 ccaccgctgg cagcgtcgat gtgcatgtcg atgtcgatac cggtgtcggc ctggaattta
      421 tcca
//
LOCUS       DAJTSI010000434          406 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.444, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000434 DAJTSI010000000
VERSION     DAJTSI010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.444"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>406
                     /locus_tag="OO327_004399"
     CDS             <1..>406
                     /locus_tag="OO327_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6020662.1"
                     /translation="SKIYSAMQKMTNRRHEENWKLRAESCRKKGKPVPEYTPKPASVA
                     TKATHLSFIKALLRAAEREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPL
                     KSVVEFALATGLRRSNIINLEWQQINMQRRVAW"
BASE COUNT          142 a           89 c           96 g           79 t
ORIGIN      
        1 tcaaaaattt attcagcaat gcagaaaatg acgaaccggc gtcatgagga aaactggaaa
       61 ctcagggcag aatcatgcag aaaaaaaggg aaacctgttc cagaatacac gccaaaacca
      121 gcgtccgttg caacgaaggc tacgcatctt tcatttataa aggccctact aagagccgca
      181 gagcgtgaat ggaaaatgct ggataaggca ccaattatta aagtgcctca accaaagaat
      241 aaacggatcc gctggctgga gccccatgaa gcacaaaggc tgattgatga atgtccggag
      301 ccattaaagt ctgttgttga atttgcactg gcaacaggct taagacgctc gaacatcatc
      361 aaccttgaat ggcaacaaat aaatatgcag cgccgggtgg catgga
//
LOCUS       DAJTSI010000435          393 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.445, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000435 DAJTSI010000000
VERSION     DAJTSI010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.445"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(232..>393)
                     /locus_tag="OO327_004400"
     CDS             complement(232..>393)
                     /locus_tag="OO327_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020663.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          132 a           96 c           67 g           98 t
ORIGIN      
        1 cgatagtcac ggacaatact cactccctgc ttctggcaaa tatgagctgc gagtgctgca
       61 aactcgaaac gacgcccgta aaaacaaaac taaaaaatat aacgtcgata ttcaaattaa
      121 gtaaatgtca accgacgggg actatccccg tcatagacgt taacgcccca ctcctctctg
      181 tcgctcaaga ggttaatgac gaatagcaga gaacataatg taaacaaggc atcactcacg
      241 acacataaat aacatctttg tggtggtttg attgattttt ccactccgct taccatctac
      301 accaaaataa cgatcatcac gacataattt ttctttaaca tcgcaaaata caccgttagc
      361 gaatgtaaat tcgctgggat cgtagccttg taa
//
LOCUS       DAJTSI010000436          388 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.446, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000436 DAJTSI010000000
VERSION     DAJTSI010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.446"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(40..>388)
                     /locus_tag="OO327_004401"
     CDS             complement(40..>388)
                     /locus_tag="OO327_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6020664.1"
                     /translation="QIKPFLEKAMTMYNDVLFPGEDINKPISIAAANRFVNRIRGGMD
                     LGYWRTHDFRRTLVTRLSEMNVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELH
                     ADKLFWHIRSISD"
BASE COUNT           92 a           88 c           87 g          121 t
ORIGIN      
        1 ttcgtgaagc tgttgaaggg tggattctta acggtggcgt taatcagaaa tgctcctgat
       61 gtgccagaac aatttatcag cgtgaagctc atacgctttg cgctgagctt ctatccagtc
      121 gtgtttattg tatacggaca ttatcccgcc aagttcatga ccgagcattc gctcagtaac
      181 atgaggctcg acattcatct cggacagccg tgtaacaagc gttcttctga aatcatgtgt
      241 tcgccagtaa cctagatcca tccctcccct tattctattt acgaatcggt tggctgcagc
      301 gatgctgatc ggtttgttta tgtcttctcc agggaaaaga acatcattgt acattgtcat
      361 ggccttttcg agaaaaggct taatttgt
//
LOCUS       DAJTSI010000437          384 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.447, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000437 DAJTSI010000000
VERSION     DAJTSI010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.447"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..252)
                     /locus_tag="OO327_004402"
                     /pseudo
     CDS             complement(1..252)
                     /locus_tag="OO327_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           88 a          123 c           86 g           87 t
ORIGIN      
        1 ctcactgtct ccgttatggc gtacaccaca tcttccagta atcgccgctt caccggactc
       61 aggaccccgt atcaccatcc ggaacccctt atccgccaca tgccaggaga acctttccat
      121 aaactcgtcg ccactcatca caaggttttc ttctcgtttt gtccggtggc tgttgtaacg
      181 caggccaatt tcatcctgac cggcataatg ttccaggcgg ctcatctgca ccggctgctt
      241 ctccagccag ttaagccatc ggtttccctg ctgaaaaata tcggcaaaac ggggaagcat
      301 cagaaaggcg gaataacgcc atccggccag tgaaccgtac cgcattcagg gcaatacatg
      361 ccgtcggcgt tgataccggc agga
//
LOCUS       DAJTSI010000438          375 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.448, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000438 DAJTSI010000000
VERSION     DAJTSI010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.448"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>375
                     /locus_tag="OO327_004403"
     CDS             <1..>375
                     /locus_tag="OO327_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6020665.1"
                     /translation="DGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDG
                     MPAINAKRIYRLMRQNALLLERKLAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNG
                     EKLRVTFALDCCDRKALHWAVTT"
BASE COUNT           86 a           96 c          110 g           83 t
ORIGIN      
        1 agatggtcgc cgcagccgtc actcagatga tacggatgtg cttctccgta tacaccatgt
       61 tatcggagag ctgcccacat atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
      121 cgaacttgat ggtatgcctg cgatcaatgc caaacgtatt taccggctca tgcgccagaa
      181 tgcgctgttg cttgagcgaa aactcgctgt accgccatcg aaacgggcac ataccggcag
      241 agtggctgtg aaagaaagta atcagcgatg gtgctctgac gggttcgagt tccgctgtga
      301 taacggagaa aaactgcgag tcacgttcgc gctggactgc tgtgaccgta aggcactgca
      361 ctgggcggtc actac
//
LOCUS       DAJTSI010000439          375 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.449, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000439 DAJTSI010000000
VERSION     DAJTSI010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.449"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
BASE COUNT           93 a           85 c           76 g          121 t
ORIGIN      
        1 atggttttca ctctgaaatc ccctgaattt aatcacgttt cctttactga aggcaatttt
       61 aaccgtagta tcaattatta acccttgggc ttgatatccc tctcctcttc tgggcacggg
      121 ggatgtcaga ttacttatta cagaaaccaa aagaattgta agcgtcatct cagtgtcgtc
      181 tatttcaatg tctgatgatt ggcgatgata gagtcctctt ataacgttaa tggactctat
      241 caatgtcaga ttctcttcgc ccccgcagga ttcgtgcctc ttactctatg actctatgga
      301 gtttaagctt gatcttattg aaaaatctta ccagctaggg gcctgcgtgg cccaactggc
      361 cagagagtac gggat
//
LOCUS       DAJTSI010000440          373 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.450, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000440 DAJTSI010000000
VERSION     DAJTSI010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.450"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>373)
                     /locus_tag="OO327_004404"
     CDS             complement(<1..>373)
                     /locus_tag="OO327_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6020666.1"
                     /translation="ASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVI
                     WRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNAS
                     YQVAAYLADEIAKLGPYEFICT"
BASE COUNT           94 a          109 c          104 g           66 t
ORIGIN      
        1 gtacagatga actcatacgg ccccagtttg gcgatctcat ccgccagata ggcagcgacc
       61 tggtaggaag cattctgtac tttggtatag ccttcacgac caaggcgcag gaattcatag
      121 tactgtgcaa tcacctgacc cgccgggcgg gagaagttga tggcaaaagt accaatctga
      181 ccaccgaggt agtcaacgtt gaacaccagt tcctgcggca gcgcttcttc gtcacgccag
      241 ataacccagc cgcagcccag cggagccaga ccgaatttat ggcctgaagc actgatcgat
      301 ttcacacgcg gcaggcggaa gtcccagacg atatccgggg cgacgaacgg tgccagaaag
      361 ccaccgctgg cgg
//
LOCUS       DAJTSI010000441          372 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.451, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000441 DAJTSI010000000
VERSION     DAJTSI010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.451"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..311)
                     /locus_tag="OO327_004405"
     CDS             complement(<1..311)
                     /locus_tag="OO327_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6020667.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT           99 a           74 c           96 g          103 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa ga
//
LOCUS       DAJTSI010000442          368 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.452, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000442 DAJTSI010000000
VERSION     DAJTSI010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.452"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..255
                     /locus_tag="OO327_004406"
     CDS             <1..255
                     /locus_tag="OO327_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6020668.1"
                     /translation="IGRLMNISQAMIIPLFPVYDEKKHLLTIEIRPPMDACIASADNK
                     TIARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP"
BASE COUNT          123 a           74 c           79 g           92 t
ORIGIN      
        1 attggacgtc tgatgaacat cagtcaggct atgattatac cacttttccc ggtttatgat
       61 gaaaaaaaac atctactgac tattgaaatt cggccaccaa tggatgcttg cattgccagc
      121 gcggacaata aaacgattgc ccgacaaatg aacaaaacag tggaaatttt ggtggggcca
      181 catccggaac agtatgtctg ggttttaaaa ttgttaaaaa cacgcaaatc aaacgaagcg
      241 gacccgtacc cttgaaatta taaagtacaa gtatttccac tagttgtttg taagcgtaca
      301 gcctgaaccg tctggtcaga atctgacgaa ttagacaaag tggtgtccac caaataagta
      361 gtgggaac
//
LOCUS       DAJTSI010000443          363 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.453, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000443 DAJTSI010000000
VERSION     DAJTSI010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.453"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>363)
                     /locus_tag="OO327_004407"
     CDS             complement(<1..>363)
                     /locus_tag="OO327_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6020669.1"
                     /translation="QKSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPE
                     DVIRFDTKSDFLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLSIRWTDARPETTEPRY
                     RAKSLTFYGINGPIYHTR"
BASE COUNT           81 a           98 c           98 g           86 t
ORIGIN      
        1 agcgggtgtg ataaatgggg ccgttaatgc cgtaaaaagt aagggatttg gctctgtacc
       61 gcggttctgt tgtctcaggg cgtgcatcag tccaccggat gctgagcacc ccttcaaacc
      121 gtgtgtcggg tatgatgatg ccgtaggggc cagcaacgga atattcacct ggcagcgcat
      181 tccttaccca ggccaggaaa tcactcttag tgtcaaaacg gataacatct tcaggcagaa
      241 aagcacaccc aaagccgaat gcgccgggta tcgccagacg gccttttgtc cggtcgtaaa
      301 tgtcgctctg tgcttccatc gtggccgcac ttttcagccc cagattatcc cgggacttct
      361 gtt
//
LOCUS       DAJTSI010000444          353 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.454, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000444 DAJTSI010000000
VERSION     DAJTSI010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.454"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..264
                     /locus_tag="OO327_004408"
     CDS             <1..264
                     /locus_tag="OO327_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6020670.1"
                     /translation="FYRNDEFVFDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISD
                     EDKEKLKACRIYAKSLQAMDFSTITDKSSYNAIEWPASPEGSS"
     gene            288..>353
                     /locus_tag="OO327_004409"
     CDS             288..>353
                     /locus_tag="OO327_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6020671.1"
                     /translation="MSVVISGALTDGAGIPMSGYHI"
BASE COUNT           96 a           80 c           90 g           87 t
ORIGIN      
        1 ttttaccgca atgatgaatt tgtctttgac tacagactca aagcggaaga tgagcgtgat
       61 gcccttctgg ctcaggtcag tgcccggaca ggggaatggg aagaagacct gctgctgggg
      121 ttaatcagcg acgaagataa agaaaagctg aaagcctgtc gtatttacgc gaaatcgctg
      181 caggcgatgg atttcagcac catcactgat aaatcctcat acaacgccat tgaatggccc
      241 gcctctccgg aaggttcttc ctgatttaat ttatcgcgag aaaaacaatg tctgtagtga
      301 tatcaggtgc gctgactgat ggcgcaggta tccccatgtc cggataccat att
//
LOCUS       DAJTSI010000445          350 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.455, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000445 DAJTSI010000000
VERSION     DAJTSI010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.455"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            163..>350
                     /locus_tag="OO327_004410"
     CDS             163..>350
                     /locus_tag="OO327_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6020672.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWIN"
BASE COUNT           97 a           97 c           82 g           74 t
ORIGIN      
        1 gtgaaactgg gctgcatccg actcttttat caacgcttac gtaattaagg gacacagcct
       61 aaatagctgc gcggaatagt agatcccttt gagggaactt agcccggatt gtgcgatctg
      121 atcaatcgcc aaatcaaaac aaatcaccaa ccggactgag caatgccgat catagcacca
      181 atttcccgtg acgaacgacg cctgatgcag aaagccatcc ataaaacaca cgataaaaat
      241 tatgcccgca gactgactgc catgctgatg ctgcaccggg gcgaccgtgt cagcgacgtt
      301 gccagaacgc tctgctgcgc ccgttcctct gttggacgct ggattaactg
//
LOCUS       DAJTSI010000446          339 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.456, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000446 DAJTSI010000000
VERSION     DAJTSI010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.456"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            3..170
                     /gene="xisR"
                     /locus_tag="OO327_004411"
     CDS             3..170
                     /gene="xisR"
                     /locus_tag="OO327_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014884030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HCR6020673.1"
                     /translation="MLRTGLGARQIEAYRQNCWVEGFHFKRVSPLGKPDSKRGIIWYN
                     YPKINQFIKDS"
     gene            172..>339
                     /locus_tag="OO327_004412"
     CDS             172..>339
                     /locus_tag="OO327_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HCR6020674.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHQISS"
BASE COUNT          108 a           63 c           82 g           86 t
ORIGIN      
        1 tgatgcttcg cactggtctg ggtgccagac aaattgaagc ataccgccag aactgttggg
       61 tggaaggctt ccacttcaaa cgagtatctc ctttagggaa gccagacagt aagcgaggga
      121 ttatctggta caactatccg aagataaatc agtttatcaa agactcatga tatgtctaaa
      181 ttaccaacag gtgtcgagat tcgaggtaaa tacattcgca tctggttcat gtttcgagga
      241 aaacgatgtc gggaaacatt gaaaggctgg gagattacta acagtaacat taaaaaggcc
      301 gggaatttaa gagcgttgat agttcatcaa atcagttcc
//
LOCUS       DAJTSI010000447          332 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.457, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000447 DAJTSI010000000
VERSION     DAJTSI010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.457"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            51..>332
                     /locus_tag="OO327_004413"
     CDS             51..>332
                     /locus_tag="OO327_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR6020675.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPD"
BASE COUNT           83 a           70 c           98 g           81 t
ORIGIN      
        1 cgtccgaatg ttagccattc gagggttgtt aagtagtttc aggaggattt atggcgaaac
       61 cttttactcc cgaacagcgg gaagaactga agacgcgaat tgtggaactc gtgcatcagg
      121 acggtcgggt cacgattcgg cagttgtccg atgaaacagg tatcagtcgt gcgtctgtcg
      181 gtcgcttatg catagaactg gtcgcaagtg gtgatgtata taattctggc tacggcttat
      241 tcccgtctga acaggctcgc aaggactggc aaagcgcccg caaaaaactc tcgagagtaa
      301 aggtgaggaa accggttgtt gttgatccgg ac
//
LOCUS       DAJTSI010000448          331 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.458, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000448 DAJTSI010000000
VERSION     DAJTSI010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.458"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(43..330)
                     /locus_tag="OO327_004414"
     CDS             complement(43..330)
                     /locus_tag="OO327_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020676.1"
                     /translation="MDEEYRKDLQLWFGLTHASFCVMPRVFMEAMPPEWQEKMAQLLF
                     EYSDTIKTDVCGVHSCFVTAKDGNNRFMRMPEDILNYRHPRREFIESFLKK"
BASE COUNT           91 a           85 c           66 g           89 t
ORIGIN      
        1 taagccatta tcaaacccac ccgtagatag gctttgtaat ggctacttct tcagaaatga
       61 ttcgatgaat tcacgtcgag gatgacgata gttcagaata tcttctggca tcctcataaa
      121 gcggttgttg ccgtctttgg cagtaacaaa acagctgtga actccgcaga catccgtttt
      181 gatcgtgtcg ctatactcaa aaagcaactg agccatcttc tcttgccatt ctggaggcat
      241 agcctccatg aatactcgcg gcatcacgca gaacgacgca tgcgtaagac caaaccacag
      301 ttgcaggtct ttacgatatt cttcatccat c
//
LOCUS       DAJTSI010000449          331 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.459, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000449 DAJTSI010000000
VERSION     DAJTSI010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.459"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>331
                     /locus_tag="OO327_004415"
     CDS             <1..>331
                     /locus_tag="OO327_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6020677.1"
                     /translation="GKVIAPACTLVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPI
                     ALEGCDVTVTKNATFTFSGTADGVQPTAFANQATTDAATNVALQMYLPDGSTSVTPGT
                     ETSNIQLA"
BASE COUNT           90 a           79 c           77 g           85 t
ORIGIN      
        1 tggtaaagtt attgctccag cttgtaccct ggtagctgcg acgaaagatt cagtagtcac
       61 attgccaaat gtcagcgcca ccaagttgca aactaatgga gcagtctctg gcgttaaaac
      121 tgatgtacca attgccttag aaggctgtga tgttactgta actaaaaatg cgacgtttac
      181 ctttagtggt acggctgacg gcgtgcaacc tacagcattt gccaaccagg ctaccaccga
      241 tgcagcgacg aatgttgctc tacaaatgta tctgccagat ggttctacat cggtaactcc
      301 cggtaccgaa acgtcgaata ttcagttagc g
//
LOCUS       DAJTSI010000450          328 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.460, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000450 DAJTSI010000000
VERSION     DAJTSI010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.460"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..83)
                     /locus_tag="OO327_004416"
     CDS             complement(<1..83)
                     /locus_tag="OO327_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HCR6020678.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFLQW"
BASE COUNT           96 a           84 c           62 g           86 t
ORIGIN      
        1 aaccactgca aaaaccagta gccggcactg ccggcggagg tgccgtaggc aatgcctgtt
       61 gatatttttt ccatctgata catatcccgc ccccccgaca gacctgtgct atcggaaaga
      121 aaaaaggcca tcagcaaaac tctgatggcc tgaatcacct ttaccaatat tgtatgaaaa
      181 aacacgcacg actaattgac aataattttc atttccatta aaaaagatcg cgtagcattc
      241 ttaattcatg aggaacttta cccgccagca atctgagtag cgaaagctgt tcatccccaa
      301 tgtttttgct ggcgggtcct tttttcta
//
LOCUS       DAJTSI010000451          325 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.461, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000451 DAJTSI010000000
VERSION     DAJTSI010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.461"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
BASE COUNT          109 a           56 c           62 g           98 t
ORIGIN      
        1 aatttacgta gctcacgctt aacagagaaa atacttatat atcaaaggct tggttaaaaa
       61 tgattaaaat gcgctaagtc tttggaatca aaagagaatt taatgattta ctctaatcag
      121 cttcgttaca ctggcaaaga tggtgtttac ttccatctac aaggccaacc agataatttc
      181 ttgtcactat cagcaactga aaacatggaa tttgatagcc ctatgatggt taccactcaa
      241 aatatgcaat cagggcagac tgtaacagat aatgtaatgc gttcacctag aactatttca
      301 atcagtggtg tggttgtggt tggtg
//
LOCUS       DAJTSI010000452          325 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.462, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000452 DAJTSI010000000
VERSION     DAJTSI010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.462"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
BASE COUNT           70 a           92 c           79 g           84 t
ORIGIN      
        1 gattcaggca ccagaatcac ccgacgcccc ggaggcgctg ccggggtgtc gttccatttc
       61 tgatgatgac ctgtgcgcct ggtgtacccg tctgctttac cgtcccggag aaatcagtct
      121 gtgcagactg agtatacagg caatggcatc tggccatccg tctgtgaccg gaacggttac
      181 gcgtacggct gtccggaatt tcagccgaat atcattcatc cctgatatta ccgggtcatc
      241 acggtcagtg atgaacgtga tgttgccagc tcccgacctt tactccctct catttattca
      301 cattacaagg atttcataca tgaaa
//
LOCUS       DAJTSI010000453          319 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.463, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000453 DAJTSI010000000
VERSION     DAJTSI010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.463"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>319
                     /locus_tag="OO327_004417"
     CDS             <1..>319
                     /locus_tag="OO327_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR6020679.1"
                     /translation="PFVAPSAVIQARQRLGSEAVRRVFTKTAQLWHNATPHPHWCGLT
                     LLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQMELTSHLLTAAAFGTMK
                     NSEN"
BASE COUNT           66 a          110 c           90 g           53 t
ORIGIN      
        1 cccttcgttg cccccagtgc cgttattcag gcccgccagc gcctgggaag tgaggctgtc
       61 cgccgcgtgt tcacgaaaac agcgcagctc tggcataacg ccacgccgca tccgcactgg
      121 tgcggcctga ccctgctggc catcgatggt gtgttctggc gcacaccgga tacaccagag
      181 aacgatgcag ccttcccccg ccagacacat gccgggaacc cggcgctcta cccgcaggtc
      241 aaaatggtct gccagatgga actgaccagc catctgctga cggctgcagc cttcggcacg
      301 atgaagaaca gcgaaaatg
//
LOCUS       DAJTSI010000454          319 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.464, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000454 DAJTSI010000000
VERSION     DAJTSI010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.464"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(31..89)
                     /locus_tag="OO327_004418"
                     /pseudo
     CDS             complement(31..89)
                     /locus_tag="OO327_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
BASE COUNT           70 a           95 c           72 g           82 t
ORIGIN      
        1 gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta cgagtaacaa
       61 ccaaccagta tgatgacgaa cttcatcata accctttcct tctataaggc cccttcctcg
      121 ggaggggctt tcccgtttca gccccttgct gactccccga ttgttgatga gcgccggacc
      181 ccgcgcaacg ctttccctga agcagttttg ccttccccga gcggtcacgc aaaacgctgc
      241 aacgtcctga gcatttgcac cttttagcgg aacgcagata taaaaaatcc ccggcttttt
      301 gggccaggga tatgtgtta
//
LOCUS       DAJTSI010000455          304 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.465, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000455 DAJTSI010000000
VERSION     DAJTSI010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.465"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>304
                     /locus_tag="OO327_004419"
     CDS             <1..>304
                     /locus_tag="OO327_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6020680.1"
                     /translation="IEAEVRGCSAEQRLAARKARAAPLMQSLYDRIQQQMKTLSRHSD
                     TAKAFAYLLKQWKALNVYCSNGWVEIDNNIAENALRAVSLGRKNFLFFGSDHGGERG"
BASE COUNT           82 a           75 c           91 g           56 t
ORIGIN      
        1 ccatcgaggc agaagtccgg ggatgttcag cagaacagcg tctggcggca agaaaagcaa
       61 gagccgcgcc actgatgcag tcactgtatg accggataca gcaacagatg aaaacactgt
      121 cgcgtcactc ggatacggca aaagcgttcg catacctact gaaacagtgg aaggccctga
      181 acgtgtactg cagtaatggc tgggtggaaa tcgacaacaa catcgctgaa aatgccctgc
      241 gggcggtcag tctgggtcgt aaaaacttcc tgttcttcgg ctctgaccat ggtggtgagc
      301 gggg
//
LOCUS       DAJTSI010000456          301 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.denovo.466, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000456 DAJTSI010000000
VERSION     DAJTSI010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.denovo.466"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            61..>301
                     /locus_tag="OO327_004420"
     CDS             61..>301
                     /locus_tag="OO327_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HCR6020681.1"
                     /translation="MAELAIELDDMTIITEKLRDMKNALSKLNHDIEEDTKRIKAITT
                     GSLKDIDRTTKEGMVETQLLIKGVLKEIVIDAAKRR"
BASE COUNT          117 a           55 c           64 g           65 t
ORIGIN      
        1 actgacacta ttgatagcct taaattacag cgtgaagatt tgcagcgtaa aatctcaaaa
       61 atggcagaac ttgctattga actcgatgat atgacaatta tcacagagaa attacgcgac
      121 atgaagaatg cgctttcaaa actcaatcat gatatcgaag aggatacaaa gcggataaag
      181 gccatcacaa ctggaagcct caaagatatt gacaggacaa caaaggaagg gatggtagag
      241 acacagctac ttatcaaggg cgtactcaaa gaaatagtta tagatgccgc taaacgtcga
      301 t
//
LOCUS       DAJTSI010000457         6119 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.guided.187, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000457 DAJTSI010000000
VERSION     DAJTSI010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6119
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.guided.187"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            263..922
                     /locus_tag="OO327_004421"
     CDS             263..922
                     /locus_tag="OO327_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR6020682.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1123..1500)
                     /locus_tag="OO327_004422"
     CDS             complement(1123..1500)
                     /locus_tag="OO327_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6020683.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1567..4533)
                     /locus_tag="OO327_004423"
     CDS             complement(1567..4533)
                     /locus_tag="OO327_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR6020684.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            complement(4536..5096)
                     /locus_tag="OO327_004424"
     CDS             complement(4536..5096)
                     /locus_tag="OO327_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6020685.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(5222..5572)
                     /locus_tag="OO327_004425"
     CDS             complement(5222..5572)
                     /locus_tag="OO327_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR6020686.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(5775..>6119)
                     /gene="intI1"
                     /locus_tag="OO327_004426"
     CDS             complement(5775..>6119)
                     /gene="intI1"
                     /locus_tag="OO327_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR6020687.1"
                     /translation="GHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQ
                     AGITKPATPHTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRS
                     PLDALPPLTSER"
BASE COUNT         1238 a         1696 c         1837 g         1348 t
ORIGIN      
        1 caccatcata cactaaatca gtaagttggc agcatcaccc gacgcacttt gcgccgaata
       61 aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac
      121 cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc
      181 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
      241 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
      301 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
      361 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
      421 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
      481 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
      541 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
      601 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
      661 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
      721 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
      781 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
      841 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
      901 tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg
      961 ttgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcga aagcaaattc
     1021 gacccggtcg tcggttcagg gcagggtcgt taaatagccg cttatgtcta ttgctggttt
     1081 accggtttat tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc ctgaggccag
     1141 tttgctcagg ctctccccgt ggaggtaata attgacgata tgatcattta ttctgcctcc
     1201 cagagcctga taaaaacggt tagcgccggg gttggatttt tcagcgttcc agctaaggct
     1261 aaggcattcc tgttcaagcg caagccgggc tataaagcgc attatcgctt tacccgtgcc
     1321 cttatttcga tcgcactgag aaacatacag ctctttaata tgcagctgac cgctgtatcg
     1381 gggcgaggga taaagaatat tgcaacatgc caggccagta atgttattgc cgcagcgcgc
     1441 tctgatcacc agggtaccgg aaagccggtt gaataacttt ttacagagat aatccttcat
     1501 caacgcttcc tgaatgatac cttcgccata ataggggtcg tctcagaaaa cggaaaataa
     1561 agcacgctaa gccggttgca gaggccgtag cggcctgaac ttccccgcgc cgatcttggc
     1621 gctgctgcgc cataggtaat caccggtcag gttgatgtgc tcccagccga gtggcgacag
     1681 gtactgcaat agcgagtcat cgacggcatg accattgccg cgcaacgcat gcgccgcacg
     1741 ctccaggtag accgtgttcc acagcacgat ggccgccgtc accaggttga ggccgctggc
     1801 ccggtagcgc tgctgctcga aactgcggtc acggatttca ccaaggcggt tgaagaacac
     1861 ggcacgggcc agcgcattgc gcgcctcgcc cttgttcagc ccggcatgca cgcggcggcg
     1921 tagctcgacg ctttgcagcc agtcgaggat gaacagcgtg cgctcgatgc ggcccaactc
     1981 gcgcagcgcg acggccaagc cgttctggcg cgggtagctg ccgagtttcc tgagcatcag
     2041 cgaggccgtc accgtgccct gcttgatcga ggtggccagc cgcaggattt cgtcccaatg
     2101 ggcgcggacg tgcttgatgt tgagcgtgcc gccgatcatc ggcttgagcg cgtcataggc
     2161 ggcatcgccc ttcgggatgt agagcttggt gtcgcccagg tcgcggatgc gcggcgcgaa
     2221 gcggaagccc aagaggtgca tcagggcgaa gacgtgatcg gtgaagcccg ccgtgtcggt
     2281 gtagtgctcc tcgatccgca ggtcggattc gtggtacagc aggccgtcga gcacgtaggt
     2341 tgagtcgcgc aggccgacat tgaccacctt ggtgtggaat ggcgcgtatt ggtcggagat
     2401 gtgggtgtag aaagtccgtc ctgggctgct gccatatttt gggttgatgt gccccgtgct
     2461 ctttgccttg ctagcggttc ggaaattctg tccgtccgat gatgatgtgg tgccatcgcc
     2521 ccagtgcccg gcaaagggat gccgaaactg agcgttgacc agttcagcca acgctgtcga
     2581 gtacgtttcg tcgcgggtat gccaggcttg cagccaagcg agcttcgcgt aggtcgtgcc
     2641 ggggcaggac tcggccatct tggtcaggcc caggttgatc gcgtcggcca ggatcgtggt
     2701 caacaacagg ttcttgtcct tggccagatc gcccgatttc aagtgcgtga agtgccgggt
     2761 gaagcccgtc cactcatcga cttcgagcag cagttcggtg atcttgacgt gcggcaggac
     2821 catggctgtc tggtctatca gcgcctgcgc ggtgtcgggc accgccgcat ccagcggcgt
     2881 gatcttcaag cccgactcgg tgatgatggc atccggcagg tcgttggctg ccgccatgcg
     2941 gttgacggtg gcaagttgtg cttccagcag cgtcagccgc tcatgcagat attgttcgca
     3001 gtcggtggcc acggccagcg gcaattcgct ggactgcttg aggctggtga acttctcggg
     3061 cggtaccagg tagtcctcga agtccttgaa ctggcgtgaa ccctgcaccc agatgtcgcc
     3121 cgagcgcagg gagttcttca actcggacag cgcgcacagt tcgtagtagc gccggtcgat
     3181 gccggcgtcg gtcatcacca gtttctgcca gcgcggcttg atgaagccgg tcggtgcatc
     3241 ggctggcagc ttgcgggcgt tgtcggtgtt catgccgcgc agcacctcaa tggcatcaag
     3301 cacgtttttg gcggcgggcg cggcccgcag cttgagcacg gcaaggaatt ccggtgcata
     3361 gcggcgcagg gtggcgtagc tctcgccgat gcgatgcagg aaatcgaagt catcgggttg
     3421 cgcgagcttc tgcgcctcgg tgacgctctc ggcaaaggaa tcccaggaca tgacggcctc
     3481 gatggcggca aacgcatcgc ggcctgattg cttggcgtcg atcagcgcct gaccgatgcg
     3541 cccgtacaga cgtaccttgg cgttgatggc cttgcctgac gcctggaact gctgctgatg
     3601 cttattcttg gcagcgttaa acagcttacc caggatgcgg tcgtgcaggt cgatgatttc
     3661 gtcggtgacg gtggccatgc cctcggtggc cagcgccacg agagtggcgt agcgccgttg
     3721 cggctcgaat ttggccaggt cggcgggtgt catctggccg ccctcgcggg caatcttgag
     3781 caggcggttc tggtgaacca gccgctcgat gccggtaggc agatcgagtg cctgccatgc
     3841 cttgaggcgt tcgatgtgtt ccagcatatg ccgcgaattt ggcttggccg gagactggcg
     3901 caaccaagcc aaccaggtcg tcttgccgtt gtcccggcgc ttgagcagat cgtcgaggcg
     3961 gcggcgatgc gcgtccgcca gtggttcggc caaggcgtcg tagatgcgcc ggttagcacg
     4021 ggtgatcgcc tcggcactcg cccgctcgac ggcgttgagg gcgggcagaa tgaccgactg
     4081 ccgccgcagg tgcccgatca aggcgctggc cagcacgatg cctttgtcgg tttgcatcgc
     4141 cagctcggtc agcatctgga cggcctgccg gtaatggctc atggtgaagg gccggaaacc
     4201 gaacacggtt tgcagctcgc tcaggtgctc gcgccgggtc tgctcccgct ggccgtactc
     4261 gttccagctt tcgacgccga ccttgagctg gtcggcgacc agcttcaaca agggcgggaa
     4321 cggtagttca tcgacgccca ggatgacgcc gggaaagcgc aggtaacaga gctgcaccgc
     4381 gaagcccagc cgattggctg gcccgcgccg ctgtcggatg atcgagaggt cggtatcgtt
     4441 gaatgtgtaa tgtcggatca ggtcgtcctt ggagtccggc aacgccagca ggctttcccg
     4501 ctcggcggcg gacaggatgg aacgacgtgg catatttact gatccgttct caagtattga
     4561 tacagggttt cgcgactgat tccgaattca cgagcaagct tggtcttttg ctcgccagcc
     4621 tcgacacgtt ggcgcagttc ggcaatacgc tcagacgaca gggatttctt cctgccacgg
     4681 taagccccgc gttgcttggc gagcgcaata ccctcgcgct gacgctcgcg gatcagggcg
     4741 cgctcgaact cggcgaacgc gcccatcacc gagagcatca ggttcgccat cggagagtct
     4801 tcgccagtaa aactgaggtg ttccttgacg aattcgatat gcacgccgcg ttgtgtcagc
     4861 gtttgcacga tccggcgcaa atcatcgaga ttgcgcgcca ggcgatccat gctatgcacc
     4921 accacggtgt cgccggtgcg ggcgaagctt atcagcgctt ccagttgcgg acgcttgaca
     4981 tccttgccgg atgccttgtc gctaaaagcg cgatcaacct tgacgccttc cagttgccgt
     5041 tccgggttct ggtcgaaggt gctgaccctg atatacccaa tgcgctgtcc agtcatggaa
     5101 ttccctgcaa aatgtcaggg aagactctat gaccttcaac gagatatgtc aataaattca
     5161 aaattcaatc ctatcctgac gcaatttaca catggcatct gacatcaggt tagggtatgc
     5221 ctcaacctga cggcggcgaa tcacaagcgt ccggtttgac gctggtttcg gtcgcagtgc
     5281 tggcccgcgc ctggaagcgc tgatagcact ccagcccgca gaaatgctcc acgtactcgg
     5341 ccccttccgg cgtgaaggcg gcatcgagcg ggatttcctt gcagcacacg cagcaactgg
     5401 tgctcggttc attggcgttc atggtggtgt tcctccattg gttgacgaag ccgacgaagg
     5461 ccgccgccgg catcggcctg gcgaacagga aaccctgcac cgtgtcgcaa cccgcctgcc
     5521 gcaaccacgc aaggcagtcg ggtgtttcca caccctcggc taccacctcc attgtcgttt
     5581 tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg gaggggttgg
     5641 atccatcagg caacgacggg ctgctgccgg ccatcagcgg acgcagggag gactttccgc
     5701 aaccggccgt tcgatgcggc accgatggcc ttcgcgcagg ggtagtgaat ccgccaggat
     5761 tgacttgcgc tgccctacct ctcactagtg aggggcggca gcgcatcaag cggtgagcgc
     5821 actccggcac cgccaacttt cagcacatgc gtgtaaatca tcgtcgtaga gacgtcggaa
     5881 tggccgagca gatcctgcac ggttcgaatg tcgtaaccgc tgcggagcaa ggccgtcgcg
     5941 aacgagtggc ggagggtgtg cggtgtggcg ggcttcgtga tgcctgcttg ttctacggca
     6001 cgtttgaagg cgcgctgaaa ggtctggtca tacatgtgat ggcgacgcac gacaccgctc
     6061 cgtggatcgg tcgaatgcgt gtgctgcgca aaaacccaga accacggcca ggaatgccc
//
LOCUS       DAJTSI010000458         5430 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.guided.205, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000458 DAJTSI010000000
VERSION     DAJTSI010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.guided.205"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            720..1535
                     /gene="sul2"
                     /locus_tag="OO327_004427"
     CDS             720..1535
                     /gene="sul2"
                     /locus_tag="OO327_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR6020688.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1596..2399
                     /locus_tag="OO327_004428"
                     /note="aph(3'')-Ib"
     CDS             1596..2399
                     /locus_tag="OO327_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR6020689.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2399..3235
                     /locus_tag="OO327_004429"
     CDS             2399..3235
                     /locus_tag="OO327_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR6020690.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3342..3746)
                     /locus_tag="OO327_004430"
                     /pseudo
     CDS             complement(3342..3746)
                     /locus_tag="OO327_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(3790..4179)
                     /locus_tag="OO327_004431"
     CDS             complement(3790..4179)
                     /locus_tag="OO327_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6020691.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4397..4837
                     /locus_tag="OO327_004432"
     CDS             4397..4837
                     /locus_tag="OO327_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR6020692.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT         1291 a         1452 c         1420 g         1267 t
ORIGIN      
        1 cctttcggtt taccggtgtc attccgctgt tatggccgcg gtttctcatt ccacgcctga
       61 cactcagttc cgtgaaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc
      121 agtccgaccg ctgcgccttg tccggtaact atcaacttga gtccaacccg gaaagacacg
      181 acaaaacgcc actggcagca gccattagta acaggagtgc acgagaagat agattaagag
      241 gtcttgaagt agaggcctga ctgcggctac actgaaagaa cagttttggt aatggcggtc
      301 tcgtgaaccc tgttaccggg ttaagcagtt ccccaactga cttaaccttc gaacaaaccg
      361 cctccccagg cggttttttc gtttacagag caagagatta cgattcattg aaaaggatct
      421 caagaagatc ctttgtagat tactatcaat taatcttccg aacatgacac cagtcactgt
      481 tgattacggt taaaccgctc cgtaacaggt ttccctcgta ttggtgactt gcagcaccaa
      541 agaagcaaaa aagataaata tatctactta ttgagagatg atatatttat ttccaaaatg
      601 ctacagatac atcatcatga aacggatcac cgcaaacagg ttactcgcct ggggattccc
      661 tttcgacccg agcatccgta tgagactcat gctcgattat tattattata gaagccccca
      721 tgaataaatc gctcatcatt ttcggcatcg tcaacataac ctcggacagt ttctccgatg
      781 gaggccggta tctggcgcca gacgcagcca ttgcgcaggc gcgtaagctg atggccgagg
      841 gggcagatgt gatcgacctc ggtccggcat ccagcaatcc cgacgccgcg cctgtttcgt
      901 ccgacacaga aatcgcgcgt atcgcgccgg tgctggacgc gctcaaggca gatggcattc
      961 ccgtctcgct cgacagttat caacccgcga cgcaagccta tgccttgtcg cgtggtgtgg
     1021 cctatctcaa tgatattcgc ggttttccag acgctgcgtt ctatccgcaa ttggcgaaat
     1081 catctgccaa actcgtcgtt atgcattcgg tgcaagacgg gcaggcagat cggcgcgagg
     1141 cacccgctgg cgacatcatg gatcacattg cggcgttctt tgacgcgcgc atcgcggcgc
     1201 tgacgggtgc cggtatcaaa cgcaaccgcc ttgtccttga tcccggcatg gggttttttc
     1261 tgggggctgc tcccgaaacc tcgctctcgg tgctggcgcg gttcgatgaa ttgcggctgc
     1321 gcttcgattt gccggtgctt ctgtctgttt cgcgcaaatc ctttctgcgc gcgctcacag
     1381 gccgtggtcc gggggatgtc ggggccgcga cactcgctgc agagcttgcc gccgccgcag
     1441 gtggagctga cttcatccgc acacacgagc cgcgcccctt gcgcgacggg ctggcggtat
     1501 tggcggcgct gaaagaaacc gcaagaattc gttaactgca cattcgggat atttctctat
     1561 attcgcgctt catcagaaaa ctgaaggaac ctccattgaa tcgaactaat attttttttg
     1621 gtgaatcgca ttctgactgg ttgcctgtca gaggcggaga atctggtgat tttgtttttc
     1681 gacgtggtga cgggcatgcc ttcgcgaaaa tcgcacctgc ttcccgccgc ggtgagctcg
     1741 ctggagagcg tgaccgcctc atttggctca aaggtcgagg tgtggcttgc cccgaggtca
     1801 tcaactggca ggaggaacag gagggtgcat gcttggtgat aacggcaatt ccgggagtac
     1861 cggcggctga tctgtctgga gcggatttgc tcaaagcgtg gccgtcaatg gggcagcaac
     1921 ttggcgctgt tcacagccta tcggttgatc aatgtccgtt tgagcgcagg ctgtcgcgaa
     1981 tgttcggacg cgccgttgat gtggtgtccc gcaatgccgt caatcccgac ttcttaccgg
     2041 acgaggacaa gagtacgccg cagctcgatc ttttggctcg tgtcgaacga gagctaccgg
     2101 tgcggctcga ccaagagcgc accgatatgg ttgtttgcca tggtgatccc tgcatgccga
     2161 acttcatggt ggaccctaaa actcttcaat gcacgggtct gatcgacctt gggcggctcg
     2221 gaacagcaga tcgctatgcc gatttggcac tcatgattgc taacgccgaa gagaactggg
     2281 cagcgccaga tgaagcagag cgcgccttcg ctgtcctatt caatgtattg gggatcgaag
     2341 cccccgaccg cgaacgcctt gccttctatc tgcgattgga ccctctgact tggggttgat
     2401 gttcatgccg cctgtttttc ctgctcattg gcacgtttcg caacctgttc tcattgcgga
     2461 caccttttcc agcctcgttt ggaaagtttc attgccagac gggactcctg caatcgtcaa
     2521 gggattgaaa cctatagaag acattgctga tgaactgcgc ggggccgact atctggtatg
     2581 gcgcaatggg aggggagcag tccggttgct cggtcgtgag aacaatctga tgttgctcga
     2641 atatgccggg gagcgaatgc tctctcacat cgttgccgag cacggcgact accaggcgac
     2701 cgaaattgca gcggaactaa tggcgaagct gtatgccgca tctgaggaac ccctgccttc
     2761 tgcccttctc ccgatccggg atcgctttgc agctttgttt cagcgggcgc gcgatgatca
     2821 aaacgcaggt tgtcaaactg actacgtcca cgcggcgatt atagccgatc aaatgatgag
     2881 caatgcctcg gaactgcgtg ggctacatgg cgatctgcat catgaaaaca tcatgttctc
     2941 cagtcgcggc tggctggtga tagatcccgt cggtctggtc ggtgaagtgg gctttggcgc
     3001 cgccaatatg ttctacgatc cggctgacag agacgacctt tgtctcgatc ctagacgcat
     3061 tgcacagatg gcggacgcat tctctcgtgc gctggacgtc gatccgcgtc gcctgctcga
     3121 ccaggcgtac gcttatgggt gcctttccgc agcttggaat gcggatggag aagaggagca
     3181 acgcgatcta gctatcgcgg ccgcgatcaa gcaggtgcga cagacgtcat actagatatc
     3241 aagcgacttc tcctatcccc tgggaacaca tcaatctcac cggagaatat cgctggccaa
     3301 agccttagcg taggattccg ccccttcccg caaacgaccc caaacaggaa acgcagctga
     3361 aacgggaagc tcaacaccca ctgacgcatg ggttgttcag gcagtacttc atcaaccagc
     3421 aaggcggcac tttcggccat ccgccgcgcc ccacagctcg ggcagaaacc gcgacgctta
     3481 cagctgaaag cgaccaggtg ctcggcgtgg caagactcgc agcgaacccg tagaaagcca
     3541 tgctccagcc gcccgcattg gagaaattct tcaaattccc gttgcacata gcccggcaat
     3601 tcctttccct gctctgccat aagcgcagcg aatgccgggt aatactcgtc aacgatctga
     3661 tagagaaggg tttgctcggg tcggtggctc tggtaacgac cagtatcccg atcccggctg
     3721 gccgtcctgg ccgccacatg aggcatgttc cgcgtccttg caatactgtg tttacataca
     3781 gtctatcgct tagcggaaag ttcttttacc ctcagccgaa atgcctgccg ttgctagaca
     3841 ttgccagcca gtgcccgtca ctcccactct tacattgttc gcagtatcta ggtacatgtt
     3901 attgcgtgca ccttcatgat gtataatgtt attaatgaca gcagaaagat cgacagaatt
     3961 aatattcttc gtacctggga cagttatcgg gtgtcggcca gtgatgagac caaaggatgc
     4021 agtataagat gaaccactac tattatcatg gccaccatta ccaccaccgt taaaactacc
     4081 tcccgaactt ccagagccgc tatgaccatc attgtagggg tcatttggtc ccatgtgacc
     4141 aaaaccaccg acagtaccgc tatttaactc tgtactcata aaattatcct ctttgacttt
     4201 taaaaacagc aaaaactgta tttataacaa tgtgtttata tacagtattc gaaatactac
     4261 gcccgtcaaa accacaccgt caacaggttt tttgtgtatt tttcacgcct cacgggcagc
     4321 ctcagacctt ttgtgttaaa acagtaatat gttctgctgt tacaatacgg tacacacagg
     4381 agacactgtc acttttatgg catggcacac agttcaagag acactgaaat taacaggaaa
     4441 gtcgcgctcg caattatatc gtgacatggc taaaggcctt gtcagctatc gcacaggaaa
     4501 aaatgatcgc cgggagttcg agaccagtga actgattcgg gcctatggcg agctgcgcac
     4561 ttctgagaca ccaaaaaggc acaaggaggg acactccaaa tctgtcgtta gccaggcgga
     4621 aaatgctcag ctagacgtca ttcagcagaa actggacgct ctgcagcaga ctgttaccct
     4681 aatgcttgaa gacaagatgg cgcgggaagc agaaaaccgc ctgcgaaatg aggagaggga
     4741 acaactacag gccgaaatta gccgtctggc tgacgcgctg gcacaggaaa aaaagcgggg
     4801 attctggtcg cggctgtttc gctccggttc agagtaacag tgcaggtgac tctgatttac
     4861 cataacatct gttatcagca ccgcctgaga ggccctcagg gcgtttctga cgggaaaccg
     4921 gtaaaacagg caacaaacca cccgaaaacc cgccagaaac gcgcaggctc tgtttttacg
     4981 gcatgcatga gtatgcgcac aagtgcatag cgcggaaatt cacaccattc agccggggta
     5041 agtcctgttt ccggcgttct tcccatccga aaacggtgaa cggggacgca atttattgcg
     5101 tggccgtgaa gcgttgccgg cgcagccgga aaacccgctg tcagcacgtt caggaagggg
     5161 gtgatgtaca aatggcacag aggatgaaag tgacgtatga gctgaaggaa tgatgtaaaa
     5221 gtgatgtata agtgatgtaa aagtgatgta tgaactgaag gagcgatgta attctgtagt
     5281 caggaagaat aacccgttca tgcaggcatg aacgatacag tgaggaacgc tttttttgtc
     5341 acgatctccc cttaacctgc gtaaatcacc cgccatggtt gattattttt caacctcaaa
     5401 atggtcatac acctaacgca gccgcgattt
//
LOCUS       DAJTSI010000459         4295 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.guided.230, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000459 DAJTSI010000000
VERSION     DAJTSI010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.guided.230"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            558..797
                     /locus_tag="OO327_004433"
     CDS             558..797
                     /locus_tag="OO327_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCR6020693.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(985..1962)
                     /gene="intI2"
                     /locus_tag="OO327_004434"
                     /pseudo
     CDS             complement(985..1962)
                     /gene="intI2"
                     /locus_tag="OO327_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2294..2767
                     /locus_tag="OO327_004435"
     CDS             2294..2767
                     /locus_tag="OO327_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR6020694.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2862..3386
                     /gene="sat2"
                     /locus_tag="OO327_004436"
     CDS             2862..3386
                     /gene="sat2"
                     /locus_tag="OO327_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR6020695.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3444..4235
                     /gene="aadA1"
                     /locus_tag="OO327_004437"
     CDS             3444..4235
                     /gene="aadA1"
                     /locus_tag="OO327_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6020696.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         1207 a          943 c         1000 g         1145 t
ORIGIN      
        1 gatcggtcaa ctgatcctta actgatcggc attacaccgg tcggcagctg tgggcggaca
       61 aaatagttgg gaactgggag gggtggaaat ggagttttta aggattattt agggaagagt
      121 gacaaaatag atgggaactg ggtgtagcgt cgtaagctaa tacgaaaatt aaaaatgaca
      181 aaatagtttg gaactagatt tcacttatct ggttaactaa tctagctaac ctgccgctca
      241 gcttagtacg atcctatatc caactgatca taatcctttc atcagtatcc caaattaaat
      301 ccccctccta gactgcctca ccaacacttt acagtgatcc cgtatttccg gacaggtgat
      361 tgattttttt cgcttgtaca ggtgttaagc cacagttatc ttgatgtgga cattatgcat
      421 tgtaatagcc cattaacatg cgctaagagc ataaattaat atacagtaac aagcattacg
      481 aggtgctaat gtttggaaag cagcctcttc caactacaat aataagttaa ctatcctcca
      541 cccacaaata attacaaatg acccctttcc gctataactc cgatctcacc agcggctcgc
      601 tacaaacccg agagtgtcgc attattactg gcttgttact ccaagagctt gatgaagctg
      661 cctgggataa agccatgtat aaggaaaatg tgcttcagaa acgcacgcaa tctacggtaa
      721 gacgtatttc ttcagcgctt agaaaacgcc tagaacacct aagctctgac ttttgggctt
      781 ttgcgttttt atgttaggca ttgtctggca actggaggaa gtgtaatgac catgaatggt
      841 cactgtgatt gacagtatct tgcttaaact tatctgaaca atggtagaac tggctcatat
      901 ttatcccctc tatgattgtt tatctttttc accataaaaa gtatcagggg ctactcaggg
      961 ctacataaag atatttatct acttttactg attgataagt agcatcagtc catccgcagg
     1021 actggtggtg ccggcaaaat gctgacccaa cacatgcgta tagatttgcg tggtcttaac
     1081 atcgttatgc cctaagagtt cttgcacagt gcgaatatca cgccccgctt gtaatagatg
     1141 cgtagcaaac gagtgacgaa atgtatgaca agtgacacgc ttgctaacga tgcctgcttt
     1201 ttgtacggct gccttcaatg cctttcgcgc aacggagtca tgcagatgat ggcggcataa
     1261 tttgccgtta tacgggtggt tgcagagcgt gctggaggga aagacaaaca tccacgccgc
     1321 ttgtcgataa gcagaagggt atttgtgatc taaagcaaaa ggcagcgatg gccctacgcc
     1381 ttgtaagttg tcgtcttgct gaataagccg cgcttgctca atgagttatt ttattgctgg
     1441 gattaggcgc gtgggcagta ggctgtttct gcttttccca cccttaccgt catgcacagt
     1501 gatgcagcca ttatcaaaat caaaatcttt aacccgcaaa cgcaagcatt cattaatgcg
     1561 caaacctgca ccatacagca gcgtaaaaat aacttggttg cgagtatcca taacctgcaa
     1621 aatgcgttgc acttcatttg cagagataac agagggtagc cgtctaggct tgcttgcagg
     1681 gatataatca atatcgccca acggctgttg taaaaacctg ttgtacaaaa aagctagggc
     1741 atttaaagcg attttctgcg tgtttatggc tacatgtctg ctgtttgcta agctggataa
     1801 aaacagcctg acctcttcac tgcccatggt ctgaggatga cgttttttgt gaaacagaat
     1861 aaaacgctta atccagtgca ggtaagtttt ttcagttttc agcgcataac ctttttgtcg
     1921 catatccgtg cgtatagaat ttaaaaatgg actgttagac ataaaacgct ccttgtcttg
     1981 caactgtctg cctatacagc ctattctagc tgggatttaa aaaagtgcct gttttttacg
     2041 cctagagatg cttgtttacc ggtagagttt taatttaatg ctaaataaat taaaatgtta
     2101 tgagttcttt gggtgagata atgtgcatcg tgcaagcagg atagacggca tgcacgattt
     2161 gtaataacag agtgtcttgt atttttaaag aaagtctatt taatacaagt gattatatta
     2221 attaacggta agcatcagcg ggtgacaaaa cgagcatgct tactaataaa atgttaacct
     2281 ctgaggaaga attgtgaaac tatcactaat ggtagctata tcgaagaatg gagttatcgg
     2341 gaatggccct gatattccat ggagtgccaa aggtgaacag ctcctgttta aagctattac
     2401 ctataaccaa tggctgttgg ttggacgcaa gacttttgaa tcaatgggag cattacccaa
     2461 ccgaaagtat gcggtcgtaa cacgttcaag ttttacatct gacaatgaga acgtattgat
     2521 ctttccatca attaaagatg ctttaaccaa cctaaagaaa ataacggatc atgtcattgt
     2581 ttcaggtggt ggggagatat acaaaagcct gatcgatcaa gtagatacac tacatatatc
     2641 tacaatagac atcgagccgg aaggtgatgt ttactttcct gaaatcccca gcaattttag
     2701 gccagttttt acccaagact tcgcctctaa cataaattat agttaccaaa tctggcaaaa
     2761 gggttaacaa gtggcagcaa cggattcgca aacctgtcac gccttttgta ccaaaagccg
     2821 cgccaggttt gcgatccgct gtgccaggcg ttaggcgtca tatgaagatt tcggtgatcc
     2881 ctgagcaggt ggcggaaaca ttggatgctg agaaccattt cattgttcgt gaagtgttcg
     2941 atgtgcacct atccgaccaa ggctttgaac tatctaccag aagtgtgagc ccctaccgga
     3001 aggattacat ctcggatgat gactctgatg aagactctgc ttgctatggc gcattcatcg
     3061 accaagagct tgtcgggaag attgaactca actcaacatg gaacgatcta gcctctatcg
     3121 aacacattgt tgtgtcgcac acgcaccgag gcaaaggagt cgcgcacagt ctcatcgaat
     3181 ttgcgaaaaa gtgggcacta agcagacagc tccttggcat acgattagag acacaaacga
     3241 acaatgtacc tgcctgcaat ttgtacgcaa aatgtggctt tactctcggc ggcattgacc
     3301 tgttcacgta taaaactaga cctcaagtct cgaacgaaac agcgatgtac tggtactggt
     3361 tctcgggagc acaggatgac gcctaacaat tcattcaagc cgacaccgct tcgcggcgcg
     3421 gcttaattca ggagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac
     3481 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     3541 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     3601 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     3661 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     3721 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     3781 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     3841 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     3901 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     3961 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     4021 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     4081 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     4141 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gttcactacg
     4201 tgaaaggcga gatcaccaag gtagtcggca aataatgtct aacaattcgt tcaagccgac
     4261 gccgcttcgc ggcgcggctt aactcaagcg ttaga
//
LOCUS       DAJTSI010000460         4292 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.guided.231, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000460 DAJTSI010000000
VERSION     DAJTSI010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.guided.231"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            558..797
                     /locus_tag="OO327_004438"
     CDS             558..797
                     /locus_tag="OO327_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCR6020697.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(985..1962)
                     /gene="intI2"
                     /locus_tag="OO327_004439"
                     /pseudo
     CDS             complement(985..1962)
                     /gene="intI2"
                     /locus_tag="OO327_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2294..2767
                     /locus_tag="OO327_004440"
     CDS             2294..2767
                     /locus_tag="OO327_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR6020698.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2862..3386
                     /gene="sat2"
                     /locus_tag="OO327_004441"
     CDS             2862..3386
                     /gene="sat2"
                     /locus_tag="OO327_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR6020699.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3444..4232
                     /gene="aadA1"
                     /locus_tag="OO327_004442"
     CDS             3444..4232
                     /gene="aadA1"
                     /locus_tag="OO327_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6020700.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         1204 a          943 c         1000 g         1145 t
ORIGIN      
        1 gatcggtcaa ctgatcctta actgatcggc attacaccgg tcggcagctg tgggcggaca
       61 aaatagttgg gaactgggag gggtggaaat ggagttttta aggattattt agggaagagt
      121 gacaaaatag atgggaactg ggtgtagcgt cgtaagctaa tacgaaaatt aaaaatgaca
      181 aaatagtttg gaactagatt tcacttatct ggttaactaa tctagctaac ctgccgctca
      241 gcttagtacg atcctatatc caactgatca taatcctttc atcagtatcc caaattaaat
      301 ccccctccta gactgcctca ccaacacttt acagtgatcc cgtatttccg gacaggtgat
      361 tgattttttt cgcttgtaca ggtgttaagc cacagttatc ttgatgtgga cattatgcat
      421 tgtaatagcc cattaacatg cgctaagagc ataaattaat atacagtaac aagcattacg
      481 aggtgctaat gtttggaaag cagcctcttc caactacaat aataagttaa ctatcctcca
      541 cccacaaata attacaaatg acccctttcc gctataactc cgatctcacc agcggctcgc
      601 tacaaacccg agagtgtcgc attattactg gcttgttact ccaagagctt gatgaagctg
      661 cctgggataa agccatgtat aaggaaaatg tgcttcagaa acgcacgcaa tctacggtaa
      721 gacgtatttc ttcagcgctt agaaaacgcc tagaacacct aagctctgac ttttgggctt
      781 ttgcgttttt atgttaggca ttgtctggca actggaggaa gtgtaatgac catgaatggt
      841 cactgtgatt gacagtatct tgcttaaact tatctgaaca atggtagaac tggctcatat
      901 ttatcccctc tatgattgtt tatctttttc accataaaaa gtatcagggg ctactcaggg
      961 ctacataaag atatttatct acttttactg attgataagt agcatcagtc catccgcagg
     1021 actggtggtg ccggcaaaat gctgacccaa cacatgcgta tagatttgcg tggtcttaac
     1081 atcgttatgc cctaagagtt cttgcacagt gcgaatatca cgccccgctt gtaatagatg
     1141 cgtagcaaac gagtgacgaa atgtatgaca agtgacacgc ttgctaacga tgcctgcttt
     1201 ttgtacggct gccttcaatg cctttcgcgc aacggagtca tgcagatgat ggcggcataa
     1261 tttgccgtta tacgggtggt tgcagagcgt gctggaggga aagacaaaca tccacgccgc
     1321 ttgtcgataa gcagaagggt atttgtgatc taaagcaaaa ggcagcgatg gccctacgcc
     1381 ttgtaagttg tcgtcttgct gaataagccg cgcttgctca atgagttatt ttattgctgg
     1441 gattaggcgc gtgggcagta ggctgtttct gcttttccca cccttaccgt catgcacagt
     1501 gatgcagcca ttatcaaaat caaaatcttt aacccgcaaa cgcaagcatt cattaatgcg
     1561 caaacctgca ccatacagca gcgtaaaaat aacttggttg cgagtatcca taacctgcaa
     1621 aatgcgttgc acttcatttg cagagataac agagggtagc cgtctaggct tgcttgcagg
     1681 gatataatca atatcgccca acggctgttg taaaaacctg ttgtacaaaa aagctagggc
     1741 atttaaagcg attttctgcg tgtttatggc tacatgtctg ctgtttgcta agctggataa
     1801 aaacagcctg acctcttcac tgcccatggt ctgaggatga cgttttttgt gaaacagaat
     1861 aaaacgctta atccagtgca ggtaagtttt ttcagttttc agcgcataac ctttttgtcg
     1921 catatccgtg cgtatagaat ttaaaaatgg actgttagac ataaaacgct ccttgtcttg
     1981 caactgtctg cctatacagc ctattctagc tgggatttaa aaaagtgcct gttttttacg
     2041 cctagagatg cttgtttacc ggtagagttt taatttaatg ctaaataaat taaaatgtta
     2101 tgagttcttt gggtgagata atgtgcatcg tgcaagcagg atagacggca tgcacgattt
     2161 gtaataacag agtgtcttgt atttttaaag aaagtctatt taatacaagt gattatatta
     2221 attaacggta agcatcagcg ggtgacaaaa cgagcatgct tactaataaa atgttaacct
     2281 ctgaggaaga attgtgaaac tatcactaat ggtagctata tcgaagaatg gagttatcgg
     2341 gaatggccct gatattccat ggagtgccaa aggtgaacag ctcctgttta aagctattac
     2401 ctataaccaa tggctgttgg ttggacgcaa gacttttgaa tcaatgggag cattacccaa
     2461 ccgaaagtat gcggtcgtaa cacgttcaag ttttacatct gacaatgaga acgtattgat
     2521 ctttccatca attaaagatg ctttaaccaa cctaaagaaa ataacggatc atgtcattgt
     2581 ttcaggtggt ggggagatat acaaaagcct gatcgatcaa gtagatacac tacatatatc
     2641 tacaatagac atcgagccgg aaggtgatgt ttactttcct gaaatcccca gcaattttag
     2701 gccagttttt acccaagact tcgcctctaa cataaattat agttaccaaa tctggcaaaa
     2761 gggttaacaa gtggcagcaa cggattcgca aacctgtcac gccttttgta ccaaaagccg
     2821 cgccaggttt gcgatccgct gtgccaggcg ttaggcgtca tatgaagatt tcggtgatcc
     2881 ctgagcaggt ggcggaaaca ttggatgctg agaaccattt cattgttcgt gaagtgttcg
     2941 atgtgcacct atccgaccaa ggctttgaac tatctaccag aagtgtgagc ccctaccgga
     3001 aggattacat ctcggatgat gactctgatg aagactctgc ttgctatggc gcattcatcg
     3061 accaagagct tgtcgggaag attgaactca actcaacatg gaacgatcta gcctctatcg
     3121 aacacattgt tgtgtcgcac acgcaccgag gcaaaggagt cgcgcacagt ctcatcgaat
     3181 ttgcgaaaaa gtgggcacta agcagacagc tccttggcat acgattagag acacaaacga
     3241 acaatgtacc tgcctgcaat ttgtacgcaa aatgtggctt tactctcggc ggcattgacc
     3301 tgttcacgta taaaactaga cctcaagtct cgaacgaaac agcgatgtac tggtactggt
     3361 tctcgggagc acaggatgac gcctaacaat tcattcaagc cgacaccgct tcgcggcgcg
     3421 gcttaattca ggagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac
     3481 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     3541 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     3601 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     3661 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     3721 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     3781 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     3841 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     3901 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     3961 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     4021 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     4081 tggagcgcct gccggcccag tatcagcccg tcatacttga agctaggcag gcttatcttg
     4141 gacaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtt cactacgtga
     4201 aaggcgagat caccaaggta gtcggcaaat aatgtctaac aattcgttca agccgacgcc
     4261 gcttcgcggc gcggcttaac tcaagcgtta ga
//
LOCUS       DAJTSI010000461         4107 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.guided.242, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000461 DAJTSI010000000
VERSION     DAJTSI010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.guided.242"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..307
                     /gene="frdD"
                     /locus_tag="OO327_004443"
     CDS             <1..307
                     /gene="frdD"
                     /locus_tag="OO327_004443"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6020701.1"
                     /translation="GAGGMWSAIIAPVMILLVGILLPLGLFPGDALSYERVLAFAQSF
                     IGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFYGLAAILTVVTLIGVVTI"
     gene            370..1503
                     /locus_tag="OO327_004444"
     CDS             370..1503
                     /locus_tag="OO327_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR6020702.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            1592..2125
                     /gene="blc"
                     /locus_tag="OO327_004445"
     CDS             1592..2125
                     /gene="blc"
                     /locus_tag="OO327_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6020703.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(2122..2439)
                     /gene="sugE"
                     /locus_tag="OO327_004446"
     CDS             complement(2122..2439)
                     /gene="sugE"
                     /locus_tag="OO327_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR6020704.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2615..2761)
                     /gene="ecnB"
                     /locus_tag="OO327_004447"
     CDS             complement(2615..2761)
                     /gene="ecnB"
                     /locus_tag="OO327_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR6020705.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2872..2997)
                     /gene="ecnA"
                     /locus_tag="OO327_004448"
     CDS             complement(2872..2997)
                     /gene="ecnA"
                     /locus_tag="OO327_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR6020706.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(3049..3615)
                     /gene="efp"
                     /locus_tag="OO327_004449"
     CDS             complement(3049..3615)
                     /gene="efp"
                     /locus_tag="OO327_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR6020707.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3657..>4107
                     /locus_tag="OO327_004450"
     CDS             3657..>4107
                     /locus_tag="OO327_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HCR6020708.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEY"
BASE COUNT         1022 a         1059 c         1033 g          993 t
ORIGIN      
        1 cggggccggt ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc tggtgggtat
       61 tctgctgcca ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg ttctggcgtt
      121 cgcgcagagc ttcattggtc gcgtattcct gttcctgatg atcgttctgc cgctgtggtg
      181 tggtttacac cgtatgcacc acgcgatgca cgatctgaaa atccacgtgc ctgcgggcaa
      241 atgggttttc tacggtctgg ctgctatcct gacagttgtc acgctgattg gtgtcgttac
      301 aatctaacgc atcgccaatg taaatccggc ccgcctatgg cgggccgttt tgtatggaaa
      361 ccagacctta tgttcaaaac gacgctctgc gccttattaa ttaccgcctc ttactccaca
      421 tttgctgccc ctcaacaaat caacgatatt gtgcatcgca caattacccc gcttatagag
      481 caacaaaaga tccccggtat ggcggtggcg gtaatttatc agggtaaacc ttattacttt
      541 acctggggct atgcggacat cgccaaaaag cagcccgtca cacagcaaac gttgtttgag
      601 ttaggttcgg tcagcaaaac atttacgggc gtgcttggtg gcgacgctat tgctcgaggg
      661 gaaatcaagt taagcgatcc cgcaacaaaa tactggcctg aacttaccgc taaacagtgg
      721 aatgggatca cactattaca tcttgcgacc tacaccgctg gcggcctgcc attgcaggtg
      781 ccggatgaag tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg gcagcctgca
      841 tgggctccag gaacacaacg tctgtatgcc aactccagta tcggtttgtt cggcgcactg
      901 gctgtgaagc cgtctggttt gagttttgag caggcgatgc aaactcgtgt cttccagcca
      961 ctcaaactca accatacgtg gattaatgta cctcccccag aagaaaagaa ttacgcctgg
     1021 ggatatcgcg aaggtaaggc agtgcatgtt tcgccagggg cgttagatgc tgaagcttat
     1081 ggtgtgaagt cgaccattga agatatggcc cgctgggtgc aaagcaattt accccccctt
     1141 gatatcaatg agaaaacgct tcaacaaggg atacaactgg cacaatctcg ctactggcaa
     1201 accggcgata tgtatcaggg cctgggctgg gaaatgctgg actggccggt caatcctgac
     1261 agcatcatta acggcagtga caataaaatt gcactggcag cacgcccggt aaaagcgatt
     1321 aagcccccaa ctcctgcagt acgcgcatca tgggtacata aaacgggggc gaccggcgga
     1381 tttggtagct atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat gctggctaac
     1441 aaaaactatc ccaatccagc gagagtcgcc gccgcctgtc agattctcaa cgctctacag
     1501 taaaatttca tcgggtccga attttcggac cttttctgcg cttttccttg ctgtcatcta
     1561 cacttagaaa aaaaccagta aggaaacatt tatgcgcctg ctccctctcg ttgccgcagc
     1621 gacagctgca tttctggtcg ttgcctgtag ttttcctacg ccgccgcgtg gcgtgaccgt
     1681 agtgaataat ttcgacgcca aacgttatct cggtacctgg tatgagattg cccgttttga
     1741 tcaccgtttt gaacgtggac tggaaaaagt caccgcaaca tacagcctgc gtgatgacgg
     1801 cggcctgaat gtcattaata aaggctataa ccctgacaga ggaatgtggc agcagagtga
     1861 agggaaagcg tactttaccg gcgcaccaac tcgcgctgcg ctgaaagtgt cattctttgg
     1921 tcctttctat ggcggttata acgttattgc actcgatcgg gaataccgcc atgcgctggt
     1981 ttgcggcccg gaccgcgact acctgtggat actctcccgc acgccaacca tttctgacga
     2041 agtgaaacag gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca gtaagtttat
     2101 ttgggtacag cagcccggta gttagtgagt gctgagtttc agaccaataa tccccaatac
     2161 gattaacgcc agactcgcca gacgcatcgg gttagcggac tcaccgagca gcacaatgcc
     2221 ggtgatggcc gcgccgactg cgccaatacc cgtccacacg gcataagccg tccctaccgg
     2281 taacgatttc atcgcccagg caagtagcgc catactgact atcatcgccg tcacggtaat
     2341 aacactcggc gtcaaacggc taaagccgtg ggtatatttc aggccaacgg cccataccac
     2401 ttccagcaga ccagcaataa ctaagataat ccaggacata tcaggctccg gaaatttggg
     2461 gccgtccccg gtgaaaagag gcgtttgcag gtcgtcctgc aaagctgatg gttgaacgga
     2521 tattttgcca ggtggcaggg gaatttcaac tattaccttt atgacgatcg cagaaaaaac
     2581 aaacggcacg acactgctgt cgtaccgtta ttgcttattg ctgcgctttc gttgctgcgc
     2641 cagaaatcgc gttaccgcca tcagaaatgt cttcaccaac gccacgcgtg gtgttgcagg
     2701 cagttaatac tgttgaaagc accagaacag aaaagatcgc tgcaattgtc ttcttcacca
     2761 taacgtcttc cttttaggta atgttattta tgtttgccta tagcaaatta ataatagaca
     2821 acatcgccaa aaatgatgga aatctgatga tttttcggaa gagaagaaaa attagctggc
     2881 agcgcgagag atggagttac cgagatgttt gatgtcttcg ccgaaaccgc aagcggtgtt
     2941 acagcccgtg agcagcgtgc tggcaagcaa aacaagaacg ataaggcgtt tcatcatctc
     3001 tgacctgaat aagtgatggt gcagcctgca ggccgcacca caaccgcatt acttcacgcg
     3061 agagacgtat tcaccagagc gggtatccac tttgatgact tcgccgattt gtacgaacag
     3121 cggaacttta accacagcgc cagtagacag ggtagccggt ttgccgccag tacctgcggt
     3181 atcacctttc aggcccggat cggtatcaac gatttccagt tcaacgaagt tcggcggagt
     3241 aacggagatc ggctgaccat tccacagagt tacgatacac tctgcctgat ccagcagcca
     3301 tttagcattg tcgccaattg cttttgcatc agcagacagc tgctcgaaag tttcgttgtt
     3361 catgaagtgc cagaactcac cgtcgttgta caggtaagtc aggttcatat cgacaacatc
     3421 agcgccttca gcggaatcag tagatttgaa ggttttttct acgcgagtac cggtcagcag
     3481 acgacgcagt ttaacgcgag caaatgcctg gcctttaccc ggttttacga attcactcgc
     3541 ttcaaccgcg taaggttcgc cgtctaacat gattttaaga ccagcacgaa aatcgttgct
     3601 atagtacgtt gccataaggc cctctgaaat ttgttaattg gtagctaagc cacaaaatgg
     3661 cgcatattgt aaccctaaat accccatcca gagaagattg gttaacgcaa cttgccgatg
     3721 ttgtgaccga tcctgatgaa cttctgcgtc ttttgaatat agacgcggac gaaaaactgt
     3781 tggccggacg cagcgccaaa aagctgtttg ccctgcgtgt gccccgctca tttatcgatc
     3841 gcatggagaa aggcaatccg gacgatcctc ttttgcgtca ggtacttacc tcgcaagatg
     3901 agtttgtcgt cgcgtccgga ttctccaccg acccactgga agaacagcac agcgtagtgc
     3961 ctggtttgtt gcataaatac cacaaccggg cgcttttgct ggtcaaaggc ggctgcgcgg
     4021 taaattgccg ctattgcttc cgtcgccact tcccctatgc cgaaaatcag ggcaacaagc
     4081 gtaactggca gactgcactt gagtatg
//
LOCUS       DAJTSI010000462         2668 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.guided.283, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000462 DAJTSI010000000
VERSION     DAJTSI010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.guided.283"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..513
                     /locus_tag="OO327_004451"
                     /pseudo
     CDS             1..513
                     /locus_tag="OO327_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(570..2027)
                     /gene="pepD"
                     /locus_tag="OO327_004452"
     CDS             complement(570..2027)
                     /gene="pepD"
                     /locus_tag="OO327_004452"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR6020709.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2289..>2668
                     /gene="gpt"
                     /locus_tag="OO327_004453"
     CDS             2289..>2668
                     /gene="gpt"
                     /locus_tag="OO327_004453"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6020710.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVV"
BASE COUNT          643 a          674 c          702 g          649 t
ORIGIN      
        1 gtcgcggtaa cggtgctgga aacagaaaca ggccgctact ctgacacgct gcgttcggcg
       61 ctgatttctc tggatggcga caacgcatgg gcgttaagcg aaagctggtg tggcactatt
      121 cagtggattt gcccgagccc gtatcgccca catcacgggc gtaaaaactg gtttctgggc
      181 attgggcgtt ttaccgctga tgagcaggag caatccgatg cgatccggta tgagacgctg
      241 cgttcgtcgg ggccgggcgg tcaacatgtc aataaaaccg actcggcggt acgcgccacg
      301 catttggcat ccggtattag cgtgaaggtt cagtcagagc gcagccagca tgctaacaaa
      361 cggctggcgc gattgctgat tgcctggaag ctggatcagc agcaatagga aaatagcgcg
      421 gtgctgaaat cacagcggcg aatgttccat caccagattg aacgtggcaa cccgcgacga
      481 acgtttacag ggatggcttt tatcgaagga taatgaagga tgaaactgcc ggaaggcgat
      541 taaacgccat ccggcagcaa atcaaataat tacttcgccg gaatttcttt cagcagttca
      601 gtcagcagtg tccagtaatg acctacgctt tcgatgtgaa cttgctcatc cggagagtgt
      661 ggaccggtga tagttggccc gatagaaacc atgtccattt ccggatacgg ttttttgaac
      721 agaccacatt ccaggcccgc gtggataatc tggatgttcg gcgtcttgtt gaacaggcgc
      781 tgataggttt cacgtaccag atgcatcacc ggagaattag cgtccggctg ccagccagga
      841 tatgcgcctt tcgcttcggt tttcgcgcca gccagtttac ccagcgaatc cagcatgctc
      901 accacgtagt ctttaccgct gtcgattagt gaacggatca ggcagtgaat ttctacgtta
      961 ttgtcagtca tggtcaccac accgacgttc agggaggttt cgaccacacc ttttgccacg
     1021 tcggaattgc ggatcacacc gttcggggtg gcgttcagca gacgaataaa ggtatcgcga
     1081 gatttcgcaa tcagggcagc tttatcgttc gctacagagt ccagcaacaa ggccagattt
     1141 ttctctttct ctgccagctc gtttttcagg atctcctgat aggtattcac cagagatttc
     1201 agtgcgtcga ctttatcagc tgcgacagca atggtcgcaa aggcttcacg cgggatggcg
     1261 ttacgcagtg tgccgccgtt gaaatcgata aggcgcaggt ccagttcttc tgcatgaccc
     1321 gccaggaagc gcaccagcag tttgttggca ttacccaggc caacgtggat ttcaccgccg
     1381 gagtgaccgc ctttcagacc ttttaaggtt aacttgaagg tttcaaaacc agctggaacc
     1441 gcttcacgat ctaaatgcag gttggaggtg aagtcgatac cccccgcaca acccatgtag
     1501 atttcacctt cttcttcgga gtcggtgtta atcagaatat cagcctgcaa ccagttgctc
     1561 tgtaagccga acgcaccgtc cataccggct tcttcggtca tggtcagcag cacttccagc
     1621 gggccgtgaa ccacgttttc gtcagccaga accgccagtg cagaggccat accaatgccg
     1681 ttatccgcac ccagcgtggt gccgcgcgct ttaacccatt cgccatcaat ataaggctgg
     1741 ataggatctt tcgtgaagtc atgcacggtg tcgttatttt tctgcggcac catatcgagg
     1801 tgggcctgta agacgaccgg tttacgattt tccatacctg cggtagcagg ttgacgaatc
     1861 aggatattac ctacctgatc gcgttcgaca tggaaacctt tctcttttgc ccaaccaaca
     1921 atgtattcag cgagttgctc ttcatgatag gacgggtgag gaatagaaca gattttggca
     1981 aaaatatccc acagcggctg tggagataat tgagacagtt cagacacgtt aagtctcctt
     2041 gtcgatcacc cgcaaaacag tattgcaggt cacagggtta gcagaaaatg ttgtcaacac
     2101 aagacaggct tgcgagatat gtattgagaa taccacttta tcccgcgtca gggagaggca
     2161 gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata
     2221 atctcgcgca acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga
     2281 cacttcacat gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta
     2341 aactcgcaag ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg
     2401 gcggtctggt accgggggcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg
     2461 tttgtatttc cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag
     2521 aaggcgatgg cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgctg
     2581 ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg
     2641 gtcgtccgct ggttgatgac tatgttgt
//
LOCUS       DAJTSI010000463         2116 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.guided.308, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000463 DAJTSI010000000
VERSION     DAJTSI010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.guided.308"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..135)
                     /locus_tag="OO327_004454"
     CDS             complement(<1..135)
                     /locus_tag="OO327_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCR6020711.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     V"
     gene            217..1422
                     /gene="tet(B)"
                     /locus_tag="OO327_004455"
     CDS             217..1422
                     /gene="tet(B)"
                     /locus_tag="OO327_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR6020712.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(1535..>2116)
                     /gene="tetC"
                     /locus_tag="OO327_004456"
     CDS             complement(1535..>2116)
                     /gene="tetC"
                     /locus_tag="OO327_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR6020713.1"
                     /translation="NHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKALVTKG
                     AFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIIDNNKN
                     LIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLNGWFM
                     HIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
BASE COUNT          546 a          390 c          442 g          738 t
ORIGIN      
        1 tacatgccaa tacaatgtag gctgctctac acctagcttc tgggcgagtt tacgggttgt
       61 taaaccttcg attccgacct cattaagcag ctctaatgcg ctgttaatca ctttactttt
      121 atctaatcta gacatcatta attcctaatt tttgttgaca ctctatcatt gatagagtta
      181 ttttaccact ccctatcagt gatagagaaa agtgaaatga atagttcgac aaagatcgca
      241 ttggtaatta cgttactcga tgccatgggg attggcctta tcatgccagt cttgccaacg
      301 ttattacgtg aatttattgc ttcggaagat atcgctaacc actttggcgt attgcttgca
      361 ctttatgcgt taatgcaggt tatctttgct ccttggcttg gaaaaatgtc tgaccgattt
      421 ggtcggcgcc cagtgctgtt gttgtcatta ataggcgcat cgctggatta cttattgctg
      481 gctttttcaa gtgcgctttg gatgctgtat ttaggccgtt tgctttcagg gatcacagga
      541 gctactgggg ctgtcgcggc atcggtcatt gccgatacca cctcagcttc tcaacgcgtg
      601 aagtggttcg gttggttagg ggcaagtttt gggcttggtt taatagcggg gcctattatt
      661 ggtggttttg caggagagat ttcaccgcat agtccctttt ttatcgctgc gttgctaaat
      721 attgtcgctt tccttgtggt tatgttttgg ttccgtgaaa ccaaaaatac acgtgataat
      781 acagataccg aagtaggggt tgagacgcaa tcgaattcgg tatacatcac tttatttaaa
      841 acgatgccca ttttgttgat tatttatttt tcagcgcaat tgataggcca aattcccgca
      901 acggtgtggg tgctatttac cgaaaatcgt tttggatgga atagcatgat ggttggcttt
      961 tcattagcgg gtcttggtct tttacactca gtattccaag cctttgtggc aggaagaata
     1021 gccactaaat ggggcgaaaa aacggcagta ctgctcggat ttattgcaga tagtagtgca
     1081 tttgcctttt tagcgtttat atctgaaggt tggttagttt tccctgtttt aattttattg
     1141 gctggtggtg ggatcgcttt acctgcatta cagggagtga tgtctatcca aacaaagagt
     1201 catcagcaag gtgctttaca gggattattg gtgagcctta ccaatgcaac cggtgttatt
     1261 ggcccattac tgtttgctgt tatttataat cattcactac caatttggga tggctggatt
     1321 tggattattg gtttagcgtt ttactgtatt attatcctgc tatcgatgac cttcatgtta
     1381 acccctcaag ctcaggggag taaacaggag acaagtgctt agttatttcg tcaccaaatg
     1441 atgttattcc gcgaaatata atgaccctct tgataaccca agagggcatt ttttacgata
     1501 aagaagattt agcttcaaat aaaacctatc tattttattt atctttcaag ctcaataaaa
     1561 agccgcggta aatagcaata aattggcctt ttttatcggc aagctctttt aggtttttcg
     1621 catgtattgc gatatgcata aaccagccat tgagtaagtt tttaagcaca tcatcatcat
     1681 aagctttaag ttggttctct tggatcaatt tgctgacaat ggcgtttacc ttaccagtaa
     1741 tgtattcaag gctaattttt tcaagttcat tccaaccaat gataggcatc acttcttgga
     1801 tagggataag gtttttatta ttatcaataa tataatcaag ataatgttca aatatacttt
     1861 ctaaggcaga ccaaccattt gttaaatcag tttttgttgt gatgtaggca tcaatcataa
     1921 ttaattgctg cttataacag gcactgagta attgtttttt atttttaaag tgatgataaa
     1981 aggcaccttt ggtcaccaac gcttttcccg agatctcatc tattgaaaca gcttgatagc
     2041 ctttttcaac aaacaatatt cgtgctgagt taaccagtga ttgataggta ctcttaaaat
     2101 tttcttgttg atgatt
//
LOCUS       DAJTSI010000464         1886 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.guided.321, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000464 DAJTSI010000000
VERSION     DAJTSI010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.guided.321"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..683)
                     /gene="intI1"
                     /locus_tag="OO327_004457"
     CDS             complement(<1..683)
                     /gene="intI1"
                     /locus_tag="OO327_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR6020714.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWP"
     gene            893..1723
                     /locus_tag="OO327_004458"
     CDS             893..1723
                     /locus_tag="OO327_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR6020715.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
BASE COUNT          572 a          463 c          426 g          425 t
ORIGIN      
        1 ggccaggaat gcccggcgcg cggatacttc cgctcaaggg cgtcgggaag cgcaacgccg
       61 ctgcggccct cggcctggtc cttcagccac catgcccgtg cacgcgacag ctgctcgcgc
      121 aggctgggtg ccaagctctc gggtaacatc aaggcccgat ccttggagcc cttgccctcc
      181 cgcacgatga tcgtgccgtg atcgaaatcc agatccttga cccgcagttg caaaccctca
      241 ctgatccgca tgcccgttcc atacagaagc tgggcgaaca aacgatgctc gccttccaga
      301 aaaccgagga tgcgaaccac ttcatccggg gtcagcacca ccggcaagcg ccgcgacggc
      361 cgaggtcttc cgatctcctg aagccagggc agatccgtgc acagcacctt gccgtagaag
      421 aacagcaagg ccgccaatgc ctgacgatgc gtggagaccg aaaccttgcg ctcgttcgcc
      481 agccaggaca gaaatgcctc gacttcgctg ctgcccaagg ttgccgggtg acgcacaccg
      541 tggaaacgga tgaaggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa
      601 tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt
      661 aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg
      721 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
      781 agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttgggcga acccggagcc
      841 tcattaattg ttagccgtta aaattaagcc ctttaccaaa ccaatactta ttatgaaaaa
      901 cacaatacat atcaacttcg ctattttttt aataattgca aatattatct acagcagcgc
      961 cagtgcatca acagatatct ctactgttgc atctccatta tttgaaggaa ctgaaggttg
     1021 ttttttactt tacgatgcat ccacaaacgc tgaaattgct caattcaata aagcaaagtg
     1081 tgcaacgcaa atggcaccag attcaacttt caagatcgca ttatcactta tggcatttga
     1141 tgcggaaata atagatcaga aaaccatatt caaatgggat aaaaccccca aaggaatgga
     1201 gatctggaac agcaatcata caccaaagac gtggatgcaa ttttctgttg tttgggtttc
     1261 gcaagaaata acccaaaaaa ttggattaaa taaaatcaag aattatctca aagattttga
     1321 ttatggaaat caagacttct ctggagataa agaaagaaac aacggattaa cagaagcatg
     1381 gctcgaaagt agcttaaaaa tttcaccaga agaacaaatt caattcctgc gtaaaattat
     1441 taatcacaat ctcccagtta aaaactcagc catagaaaac accatagaga acatgtatct
     1501 acaagatctg gataatagta caaaactgta tgggaaaact ggtgcaggat tcacagcaaa
     1561 tagaacctta caaaacggat ggtttgaagg gtttattata agcaaatcag gacataaata
     1621 tgtttttgtg tccgcactta caggaaactt ggggtcgaat ttaacatcaa gcataaaagc
     1681 caagaaaaat gcgatcacca ttctaaacac actaaattta taaaaaatct aatggcaaaa
     1741 tcgcccaacc cttcaatcaa gtcgggacgg ccaaaagcaa gcttttggct cccctcgctg
     1801 gcgctcggcg ccccttattt caaacgttaa acatcatgag ggaagtggtg atcgccgaag
     1861 tatcgactca actatcagag gtagtt
//
LOCUS       DAJTSI010000465         1554 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.guided.342, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000465 DAJTSI010000000
VERSION     DAJTSI010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.guided.342"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..590
                     /locus_tag="OO327_004459"
     CDS             <1..590
                     /locus_tag="OO327_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HCR6020716.1"
                     /translation="FDAEIIDQKTIFKWDKTPKGMEIWNSNHTPKTWMQFSVVWVSQE
                     ITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLTEAWLESSLKISPEEQIQFLRKII
                     NHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGAGFTANRTLQNGWFEGFIISKSGH
                     KYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL"
     gene            703..1494
                     /gene="aadA1"
                     /locus_tag="OO327_004460"
     CDS             703..1494
                     /gene="aadA1"
                     /locus_tag="OO327_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6020717.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          483 a          346 c          364 g          361 t
ORIGIN      
        1 catttgatgc ggaaataata gatcagaaaa ccatattcaa atgggataaa acccccaaag
       61 gaatggagat ctggaacagc aatcatacac caaagacgtg gatgcaattt tctgttgttt
      121 gggtttcgca agaaataacc caaaaaattg gattaaataa aatcaagaat tatctcaaag
      181 attttgatta tggaaatcaa gacttctctg gagataaaga aagaaacaac ggattaacag
      241 aagcatggct cgaaagtagc ttaaaaattt caccagaaga acaaattcaa ttcctgcgta
      301 aaattattaa tcacaatctc ccagttaaaa actcagccat agaaaacacc atagagaaca
      361 tgtatctaca agatctggat aatagtacaa aactgtatgg gaaaactggt gcaggattca
      421 cagcaaatag aaccttacaa aacggatggt ttgaagggtt tattataagc aaatcaggac
      481 ataaatatgt ttttgtgtcc gcacttacag gaaacttggg gtcgaattta acatcaagca
      541 taaaagccaa gaaaaatgcg atcaccattc taaacacact aaatttataa aaaatctaat
      601 ggcaaaatcg cccaaccctt caatcaagtc gggacggcca aaagcaagct tttggctccc
      661 ctcgctggcg ctcggcgccc cttatttcaa acgttaaaca tcatgaggga agtggtgatc
      721 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
      781 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt
      841 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
      901 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
      961 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
     1021 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
     1081 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
     1141 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
     1201 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
     1261 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
     1321 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
     1381 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
     1441 gaagaatttg ttcactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta
     1501 acaattcgtt caagccgacg ccgcttcgcg gcgcggctta actcaagcgt taga
//
LOCUS       DAJTSI010000466         1551 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466862-rid15819463.guided.343, whole genome shotgun
            sequence.
ACCESSION   DAJTSI010000466 DAJTSI010000000
VERSION     DAJTSI010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466862
            Sequence Read Archive: ERR10074728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477394.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477394.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:08:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,462
            CDSs (total)                      :: 4,413
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 182 of 386
            Pseudo Genes (incomplete)         :: 95 of 386
            Pseudo Genes (internal stop)      :: 149 of 386
            Pseudo Genes (multiple problems)  :: 39 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466862-rid15819463.guided.343"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-05-05"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..590
                     /locus_tag="OO327_004461"
     CDS             <1..590
                     /locus_tag="OO327_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HCR6020718.1"
                     /translation="FDAEIIDQKTIFKWDKTPKGMEIWNSNHTPKTWMQFSVVWVSQE
                     ITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLTEAWLESSLKISPEEQIQFLRKII
                     NHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGAGFTANRTLQNGWFEGFIISKSGH
                     KYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL"
     gene            703..1491
                     /gene="aadA1"
                     /locus_tag="OO327_004462"
     CDS             703..1491
                     /gene="aadA1"
                     /locus_tag="OO327_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6020719.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          480 a          346 c          364 g          361 t
ORIGIN      
        1 catttgatgc ggaaataata gatcagaaaa ccatattcaa atgggataaa acccccaaag
       61 gaatggagat ctggaacagc aatcatacac caaagacgtg gatgcaattt tctgttgttt
      121 gggtttcgca agaaataacc caaaaaattg gattaaataa aatcaagaat tatctcaaag
      181 attttgatta tggaaatcaa gacttctctg gagataaaga aagaaacaac ggattaacag
      241 aagcatggct cgaaagtagc ttaaaaattt caccagaaga acaaattcaa ttcctgcgta
      301 aaattattaa tcacaatctc ccagttaaaa actcagccat agaaaacacc atagagaaca
      361 tgtatctaca agatctggat aatagtacaa aactgtatgg gaaaactggt gcaggattca
      421 cagcaaatag aaccttacaa aacggatggt ttgaagggtt tattataagc aaatcaggac
      481 ataaatatgt ttttgtgtcc gcacttacag gaaacttggg gtcgaattta acatcaagca
      541 taaaagccaa gaaaaatgcg atcaccattc taaacacact aaatttataa aaaatctaat
      601 ggcaaaatcg cccaaccctt caatcaagtc gggacggcca aaagcaagct tttggctccc
      661 ctcgctggcg ctcggcgccc cttatttcaa acgttaaaca tcatgaggga agtggtgatc
      721 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
      781 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt
      841 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
      901 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
      961 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
     1021 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
     1081 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
     1141 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
     1201 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
     1261 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
     1321 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
     1381 gctaggcagg cttatcttgg acaagaagat cgcttggcct cgcgcgcaga tcagttggaa
     1441 gaatttgttc actacgtgaa aggcgagatc accaaggtag tcggcaaata atgtctaaca
     1501 attcgttcaa gccgacgccg cttcgcggcg cggcttaact caagcgttag a
//
